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Sample records for bronchial diseases

  1. Imaging diagnosis of bronchial asthma and related diseases

    International Nuclear Information System (INIS)

    We describe imaging features of bronchial asthma and related diseases. The practical roles of imaging diagnosis are the evaluation of severity and complications of bronchial asthma and differential diagnosis of diseases showing asthmatic symptoms other than bronchial asthma. (author)

  2. Clinical, radiographic, and bronchial cytologic features of cats with bronchial disease: 65 cases (1980-1986)

    International Nuclear Information System (INIS)

    Medical records, radiographs, and bronchial cytologic abnormalities of 65 cats with bronchial disease were reviewed. Bronchial disease was defined as abnormality of the lower airways to the exclusion of disease originating or mainly involving the alveoli, interstitium, vasculature, or pleura. Cats with bronchial disease were more likely to be female and older. Siamese cats were over represented and had more chronic disease. In order of frequency, the following clinical signs were reported: coughing, dyspnea, occasional sneezing, wheezing, and vomiting. Radiography revealed prominent bronchial markings, with some cats having collapse of the middle lobe of the right lung (n = 7), overinflation of the lungs (n = 9), or aerophagia (n = 13). Of 65 bronchial washes, 58 were considered exudative, with the predominant cell type being eosinophil in 24%, neutrophil in 33%, macrophage in 22%, and mixed population of cells in 21%. Cultures for bacteria were considered positive in 24% of the cats. Circulating eosinophilia was not helpful in predicting the predominant cell type in bronchial cytologic exudates. Hyperproteinemia without dehydration was present in a third of the cats, indicating an immunologic response. Half the cats had resolution of clinical signs, whereas half the cats required continuing medication with bronchodilators, antimicrobial agents, or corticosteroids

  3. Upper respiratory tract disease and bronchial asthma

    Institute of Scientific and Technical Information of China (English)

    1993-01-01

    930904 A clinical study on determination ofplasma vasoactive intestinal peptide and its rela-tionship with bronchial responsiveness in asth-matics.LIU Ao(刘翱),et al.Dept Respir Med,Kunming General Hosp,Kunming Command,Kunming,650032.Chin J Intern Med 1993;32(3):165-166.Vasoactive intestinal peptide(VIP),which islocalized in normal human lung,may play an im-portant role in regulating bronchial tone,pul-monary blood flow and mucus secretion.Thelevel of plasma VIP and bronchial responsivenesswere studied in patients with asthma,and chronicbronchitis and in the healthy subjects.The re-sults showed that the level of plasma VIP in

  4. Upper respiratory tract disease and bronchial asthma

    Institute of Scientific and Technical Information of China (English)

    1995-01-01

    950302 A synthetical evaluation of occupational asth-ma.LIU Jingyu(刘镜愉),et al.Occup Dis Res Cen-ter,3rd Hosp,Beijing Med Univ,Beijing,100083.ChinJ Industr Hyg & Occouat Dis 1994;12(6)322-325.The occupational exposure and medical history,physical examination,allergen bronchial provocationtest(A-BPT),skin test (ST),specific IgE(S-IgE) andIgG4(S-IgG4) were examined in 43 asthmatic patientswho were suspected to be related to their occupationa

  5. Bronchial asthma and chronic obstructive pulmonary disease: research activity in Arab countries

    OpenAIRE

    Sweileh, Waleed M; Al-Jabi, Samah W.; Zyoud, Sa’ed H; Ansam F Sawalha

    2014-01-01

    Background Chronic respiratory diseases, like bronchial asthma and chronic obstructive pulmonary disease (COPD), are a worldwide health problem. Quantitative and qualitative assessment of asthma and COPD-related research from Arab countries has not been explored and there are few internationally published reports on such field. The main objectives of this study were to analyze research output originating from Arab countries in the field of bronchial asthma and COPD. Methods Original scientifi...

  6. Mechanical ventilation in patients with chronic obstructive pulmonary disease and bronchial asthma

    OpenAIRE

    Ahmed, Syed Moied; Athar, Manazir

    2015-01-01

    Chronic obstructive pulmonary disease (COPD) and bronchial asthma often complicate the surgical patients, leading to post-operative morbidity and mortality. Many authors have tried to predict post-operative pulmonary complications but not specifically in COPD. The aim of this review is to provide recent evidence-based guidelines regarding predictors and ventilatory strategies for mechanical ventilation in COPD and bronchial asthma patients. Using Google search for indexing databases, a search...

  7. The Effectiveness of Practicing Pranayama Yoga on Some Respiratory Indicators in Patients Suffering from Bronchial Disease

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    Khue Ai Thi HOANG

    2015-06-01

    Full Text Available Bronchial asthma is considered as a large burden of disease cross the world wasting billions of dollars each year. Using drug for treatment is not only expensive but also causes many adverse health affect. The study aim s to assess the effects of pranayama yoga practice on lung functions in patients with bronchial diseases. This is a controlled trial study. After three months of yoga practice, breath indicators such as FVC, FEV 1, FEV1/FVC, PEFR of intervention group sign ificantly improved with p value < .01 to .001 in comparison wi th the previous three months of the control group. Practicing pranayama yoga is beneficial to patients with bronchial asthma.

  8. Severity-related changes of bronchial microbiome in chronic obstructive pulmonary disease.

    Science.gov (United States)

    Garcia-Nuñez, Marian; Millares, Laura; Pomares, Xavier; Ferrari, Rafaela; Pérez-Brocal, Vicente; Gallego, Miguel; Espasa, Mateu; Moya, Andrés; Monsó, Eduard

    2014-12-01

    Bronchial colonization by potentially pathogenic microorganisms (PPMs) is often demonstrated in chronic obstructive pulmonary disease (COPD), but culture-based techniques identify only a portion of the bacteria in mucosal surfaces. The aim of the study was to determine changes in the bronchial microbiome of COPD associated with the severity of the disease. The bronchial microbiome of COPD patients was analyzed by 16S rRNA gene amplification and pyrosequencing in sputum samples obtained during stable disease. Seventeen COPD patients were studied (forced expiratory volume in the first second expressed as a percentage of the forced vital capacity [FEV1%] median, 35.0%; interquartile range [IQR], 31.5 to 52.0), providing a mean of 4,493 (standard deviation [SD], 2,598) sequences corresponding to 47 operational taxonomic units (OTUs) (SD, 17) at a 97% identity level. Patients were dichotomized according to their lung function as moderate to severe when their FEV1% values were over the median and as advanced when FEV1% values were lower. The most prevalent phyla in sputum were Proteobacteria (44%) and Firmicutes (16%), followed by Actinobacteria (13%). A greater microbial diversity was found in patients with moderate-to-severe disease, and alpha diversity showed a statistically significant decrease in patients with advanced disease when assessed by Shannon (ρ = 0.528; P = 0.029, Spearman correlation coefficient) and Chao1 (ρ = 0.53; P = 0.028, Spearman correlation coefficient) alpha-diversity indexes. The higher severity that characterizes advanced COPD is paralleled by a decrease in the diversity of the bronchial microbiome, with a loss of part of the resident flora that is replaced by a more restricted microbiota that includes PPMs.

  9. Metagenomic Sequencing of the Chronic Obstructive Pulmonary Disease Upper Bronchial Tract Microbiome Reveals Functional Changes Associated with Disease Severity

    OpenAIRE

    Cameron, Simon J S; Keir E Lewis; Huws, Sharon A.; Wanchang Lin; Hegarty, Matthew J.; Lewis, Paul D.; Mur, Luis A. J.; Pachebat, Justin A

    2016-01-01

    Chronic Obstructive Pulmonary Disease (COPD) is a major source of mortality and morbidity worldwide. The microbiome associated with this disease may be an important component of the disease, though studies to date have been based on sequencing of the 16S rRNA gene, and have revealed unequivocal results. Here, we employed metagenomic sequencing of the upper bronchial tract (UBT) microbiome to allow for greater elucidation of its taxonomic composition, and revealing functional changes associate...

  10. Advances in mechanical ventilation onrespiratoryfailure as a result of chronic obstructive pulmonary disease and bronchial asthma

    Directory of Open Access Journals (Sweden)

    Zhuang MA

    2011-08-01

    Full Text Available Over years,great strides have been made in theories and practices of mechanical ventilation.The indications of non-invasive ventilation have been increasing,while invasive ventilation has been playing a vital role in patients who are critically ill with inadequate drainage of sputum and fail to benefit from non-invasive ventilation.Chronic diseases,including chronic obstructive pulmonary disease(COPD,one of the most common chronic diseases,and bronchial asthma,the incidence of which is ascending recent years,are main causes for respiratory failure.Accordingly,we focus in the present paper on discussion and elaboration of respiratory failure as a result of COPD and bronchial asthma.Type II respiratory failure is the major type of respiratory failure induced by COPD,both in acute exacerbation and stable stage,and mechanical ventilation is one of the most common treatments.Severe acute bronchial asthma complicated with respiratory failure is an emergency.In this situation,primary goal of mechanical ventilation is to make an efficient oxygenation and ventilation of patients,and to avoid hyperinflation to the utmost.For patients who are able to cooperate,non-invasive ventilation in early stage may be beneficial,avoiding tracheal intubation and application of invasive ventilation.But in those who can not achieve remission in a short term,an artificial airway should be established for invasive ventilation as soon as possible.

  11. Advances in mechanical ventilation onrespiratoryfailure as a result of chronic obstructive pulmonary disease and bronchial asthma

    OpenAIRE

    Ma, Zhuang

    2011-01-01

    Over years,great strides have been made in theories and practices of mechanical ventilation.The indications of non-invasive ventilation have been increasing,while invasive ventilation has been playing a vital role in patients who are critically ill with inadequate drainage of sputum and fail to benefit from non-invasive ventilation.Chronic diseases,including chronic obstructive pulmonary disease(COPD),one of the most common chronic diseases,and bronchial asthma,the incidence of which is ascen...

  12. ASSESSMENT OF KNOWLEDGE AND ATTITUDE OF BRONCHIAL ASTHMA PATIENTS TOWARDS THEIR DISEASE

    Directory of Open Access Journals (Sweden)

    Gaude

    2015-11-01

    Full Text Available Asthma is chronic inflammatory, airway hyper-responsiveness, reversible disorder which occurs at any age, and requires special attention towards management of drug therapy. There is lack of patients awareness in having the complete knowledge about the disease, attitude towards disease management, medication adherence behavior and treatment outcomes. The objective of the present study was to assess the knowledge and attitude of the patients towards their disease in improving the outcome in asthma patients. MATERIALS AND METHODS: This was a prospective, observational, questionnaire and hospital based study in a tertiary care teaching hospital at two different centers for a period of 12 months. The data was collected by face to face interview of outpatients and inpatients of Pulmonary Medicine and Internal Medicine departments by administering the questionnaires for the assessment of knowledge, attitude and adherence towards the bronchial asthma disease. RESULTS: A total of 160 patients with confirmed diagnosis of bronchial asthma were included in this study, out of which male patients were 87(54.5% and female patients 73(45.5%. On an average, each patient had already visited 3 doctors prior to coming to us. Out of 160 patients, 68% patients were ignorant regarding disease etiology, and another 54% patients were reluctant to accept the diagnosis of asthma. About 88% had the knowledge that lungs are affected by this disease. Another 44% opined that the medicines used for asthma can cause airways narrowing on long turn. Attitude of the patients varied greatly among the patients in this study. Regarding precipitating factors, 46% parents attributed the disease exacerbations due to multiple causes. The compliance rate in using inhalers for asthma was low (60%, and many patients stopped medications in spite of proper advice regarding inhalational therapy. CONCLUSION: This study concludes that the assessment of knowledge and attitude towards the disease

  13. Bronchial anthracofibrosis with interstitial lung disease: an association yet to be highlighted.

    Science.gov (United States)

    Kunal, Shekhar; Pilaniya, Vikas; Shah, Ashok

    2016-01-01

    Bronchial anthracofibrosis (BAF), an emerging pulmonary disease due to long-standing exposure to biomass fuel smoke, is predominantly seen in females from developing nations. BAF is known to be associated with tuberculosis, pneumonia, chronic obstructive pulmonary disease and lung cancer, but the association of BAF with interstitial lung disease (ILD) is rare and yet to be highlighted. A 72-year-old woman with a 30-year history of exposure to biomass fuel smoke presented with dry cough and exertional dyspnoea. Imaging demonstrated interlobular, intralobular and peribronchovascular interstitial thickening and honeycombing adjoining the subpleural regions, suggestive of the usual interstitial pneumonia pattern. A restrictive pattern with diffusion defect was noted. Fibrebronchoscopy revealed a bluish-black anthracotic pigmentation with a narrowed and distorted left upper lobe, and apical segment of left lower lobe bronchus, confirming BAF. A diagnosis of BAF with ILD was made. To the best of our knowledge, this is the first detailed description of this association. PMID:26759407

  14. Bronchial hyperresponsiveness and analysis of induced sputum cells in Crohn's disease

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    R.M. Bartholo

    2005-02-01

    Full Text Available With the aim of investigating the presence of latent inflammatory process in the lungs of patients with Crohn's disease, 15 patients with Crohn's disease were evaluated by spirometry, the methacholine challenge test, induced sputum, and skin tests for inhaled antigens. Serum IgE, erythrocyte sedimentation rate and hematocrit were also determined. The patients were compared with 20 healthy controls by the Mann-Whitney and Fisher exact tests. Their respiratory physical examination was normal. None had a personal or family history of clinical atopy. None had a previous history of pulmonary disease, smoking or toxic bronchopulmonary exposure. None had sinusitis, migraine, diabetes mellitus, or cardiac failure. Four (26.6% of the patients with Crohn's disease had a positive methacholine challenge test whereas none of the 20 controls had a positive methacholine test (P = 0.026, Fisher exact test. Patients with Crohn's disease had a higher level of lymphocytes in induced sputum than controls (mean 14.59%, range 3.2-50 vs 5.46%, 0-26.92%, respectively; P = 0.011, Mann-Whitney test. Patients with Crohn's disease and a positive methacholine challenge test had an even higher percentage of lymphocytes in induced sputum compared with patients with Crohn's disease and a negative methacholine test (mean 24.88%, range 12.87-50 vs 10.48%, 3.2-21.69%; P = 0.047, Mann-Whitney test. The simultaneous findings of bronchopulmonary lymphocytosis and bronchial hyperresponsiveness in patients with Crohn's disease were not reported up to now. These results suggest that patients with Crohn's disease present a subclinical inflammatory process despite the absence of pulmonary symptoms.

  15. Effect of pre-cardiac and adult stages of Dirofilaria immitis in pulmonary disease of cats: CBC, bronchial lavage cytology, serology, radiographs, CT images, bronchial reactivity, and histopathology.

    Science.gov (United States)

    Ray Dillon, A; Tillson, D M; Wooldridge, A; Cattley, R; Hathcock, J; Brawner, W R; Cole, R; Welles, B; Christopherson, P W; Lee-Fowler, T; Bordelon, S; Barney, S; Sermersheim, M; Garbarino, R; Wells, S Z; Diffie, E B; Schachner, E R

    2014-11-15

    A controlled, blind study was conducted to define the initial inflammatory response and lung damage associated with the death of precardiac stages of Dirofilaria immitis in cats as compared to adult heartworm infections and normal cats. Three groups of six cats each were used: UU: uninfected untreated controls; PreS I: infected with 100 D. immitis L3 by subcutaneous injection and treated topically with selamectin 32 and 2 days pre-infection and once monthly for 8 months); IU: infected with 100 D. immitis L3 and left untreated. Peripheral blood, serum, bronchial lavage, and thoracic radiographic images were collected from all cats on Days 0, 70, 110, 168, and 240. CT images were acquired on Days 0, 110, and 240. Cats were euthanized, and necropsies were conducted on Day 240 to determine the presence of heartworms. Bronchial rings were collected for in vitro reactivity. Lung, heart, brain, kidney, and liver tissues were collected for histopathology. Results were compared for changes within each group. Pearson and Spearman correlations were performed for association between histologic, radiographic, serologic, hematologic and bronchoalveolar lavage (BAL) results. Infected cats treated with selamectin did not develop radiographically evident changes throughout the study, were heartworm antibody negative, and were free of adult heartworms and worm fragments at necropsy. Histologic lung scores and CT analysis were not significantly different between PreS I cats and UU controls. Subtle alveolar myofibrosis was noted in isolated areas of several PreS I cats and an eosinophilic BAL cytology was noted on Days 75 and 120. Bronchial ring reactivity was blunted in IU cats but was normal in PreS I and UU cats. The IU cats became antibody positive, and five cats developed adult heartworms. All cats with heartworms were antigen positive at one time point; but one cat was antibody positive, antigen negative, with viable adult females at necropsy. The CT revealed early involvement

  16. Airway malacia in chronic obstructive pulmonary disease: prevalence, morphology and relationship with emphysema, bronchiectasis and bronchial wall thickening

    Energy Technology Data Exchange (ETDEWEB)

    Sverzellati, Nicola; Rastelli, Andrea; Schembri, Valentina; Filippo, Massimo de [University of Parma, Department of Clinical Sciences, Section of Radiology, Parma (Italy); Chetta, Alfredo [University of Parma, Department of Clinical Sciences, Section of Respiratory Diseases, Parma (Italy); Fasano, Luca; Pacilli, Angela Maria [Policlinico Sant' Orsola-Malpighi, Unita Operativa di Fisiopatologia Respiratoria, Bologna (Italy); Di Scioscio, Valerio; Bartalena, Tommaso; Zompatori, Maurizio [University of Bologna, Department of Radiology, Cardiothoracic Institute, Policlinico S.Orsola-Malpighi, Bologna (Italy)

    2009-07-15

    The aim of this study was to determine the prevalence of airway malacia and its relationship with ancillary morphologic features in patients with chronic obstructive pulmonary disease (COPD). A retrospective review was performed of a consecutive series of patients with COPD who were imaged with inspiratory and dynamic expiratory multidetector computed tomography (MDCT). Airway malacia was defined as {>=}50% expiratory reduction of the airway lumen. Both distribution and morphology of airway malacia were assessed. The extent of emphysema, extent of bronchiectasis and severity of bronchial wall thickness were quantified. The final study cohort was comprised of 71 patients. Airway malacia was seen in 38 of 71 patients (53%), and such proportion was roughly maintained in each stage of COPD severity. Almost all tracheomalacia cases (23/25, 92%) were characterised by an expiratory anterior bowing of the posterior membranous wall. Both emphysema and bronchiectasis extent did not differ between patients with and without airway malacia (p > 0.05). Bronchial wall thickness severity was significantly higher in patients with airway malacia and correlated with the degree of maximal bronchial collapse (p < 0.05). In conclusion, we demonstrated a strong association between airway malacia and COPD, disclosing a significant relationship with bronchial wall thickening. (orig.)

  17. Airway malacia in chronic obstructive pulmonary disease: prevalence, morphology and relationship with emphysema, bronchiectasis and bronchial wall thickening

    International Nuclear Information System (INIS)

    The aim of this study was to determine the prevalence of airway malacia and its relationship with ancillary morphologic features in patients with chronic obstructive pulmonary disease (COPD). A retrospective review was performed of a consecutive series of patients with COPD who were imaged with inspiratory and dynamic expiratory multidetector computed tomography (MDCT). Airway malacia was defined as ≥50% expiratory reduction of the airway lumen. Both distribution and morphology of airway malacia were assessed. The extent of emphysema, extent of bronchiectasis and severity of bronchial wall thickness were quantified. The final study cohort was comprised of 71 patients. Airway malacia was seen in 38 of 71 patients (53%), and such proportion was roughly maintained in each stage of COPD severity. Almost all tracheomalacia cases (23/25, 92%) were characterised by an expiratory anterior bowing of the posterior membranous wall. Both emphysema and bronchiectasis extent did not differ between patients with and without airway malacia (p > 0.05). Bronchial wall thickness severity was significantly higher in patients with airway malacia and correlated with the degree of maximal bronchial collapse (p < 0.05). In conclusion, we demonstrated a strong association between airway malacia and COPD, disclosing a significant relationship with bronchial wall thickening. (orig.)

  18. Metagenomic Sequencing of the Chronic Obstructive Pulmonary Disease Upper Bronchial Tract Microbiome Reveals Functional Changes Associated with Disease Severity.

    Science.gov (United States)

    Cameron, Simon J S; Lewis, Keir E; Huws, Sharon A; Lin, Wanchang; Hegarty, Matthew J; Lewis, Paul D; Mur, Luis A J; Pachebat, Justin A

    2016-01-01

    Chronic Obstructive Pulmonary Disease (COPD) is a major source of mortality and morbidity worldwide. The microbiome associated with this disease may be an important component of the disease, though studies to date have been based on sequencing of the 16S rRNA gene, and have revealed unequivocal results. Here, we employed metagenomic sequencing of the upper bronchial tract (UBT) microbiome to allow for greater elucidation of its taxonomic composition, and revealing functional changes associated with the disease. The bacterial metagenomes within sputum samples from eight COPD patients and ten 'healthy' smokers (Controls) were sequenced, and suggested significant changes in the abundance of bacterial species, particularly within the Streptococcus genus. The functional capacity of the COPD UBT microbiome indicated an increased capacity for bacterial growth, which could be an important feature in bacterial-associated acute exacerbations. Regression analyses correlated COPD severity (FEV1% of predicted) with differences in the abundance of Streptococcus pneumoniae and functional classifications related to a reduced capacity for bacterial sialic acid metabolism. This study suggests that the COPD UBT microbiome could be used in patient risk stratification and in identifying novel monitoring and treatment methods, but study of a longitudinal cohort will be required to unequivocally relate these features of the microbiome with COPD severity.

  19. CYTOKINE PROFILE AND ADHESION MOLECULES IN THE PATIENTS WITH CHRONIC OBSTRUCTIVE LUNG DISEASE COMBINED WITH BRONCHIAL ASTHMA

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    T. G. Shapovalova

    2010-01-01

    Full Text Available The study involved 56 patients with chronic obstructive pulmonary disease (COPD, stage III-IV, 38 patients with severe bronchial asthma (BA, and 45 patients with a combination of COPD stage III-IV and bronchial asthma at exacerbation period. In all the patients, we have measured blood levels of pro-inflammatory (IL-6, IL -8, TNFα and anti-inflammatory (IL-4 cytokines, amounts of cell adhesion molecules (ICAM- 1, VCAM-1, along with assessment of contractile myocardial functional disorders by means of Doppler echocardiography. Highest rates of pro-inflammatory cytokines and adhesion molecules were found in the group of patients with combined pathology, along with most significant signs of myocardial hypertrophy, both in right and left heart compartments, accompanied by a decrease in contractile myocardial function.

  20. Anxiety and depression among patients with Bronchial asthma, chronic obstructive pulmonary disease and diffuse parenchymatous lung diseases

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    Taghreed S. Farag*, Manal R. Hafez*, Taghreed Elshafie**and Omaima I. Abo-Elkheir

    2012-10-01

    Full Text Available Background: Psychosocial stressors, such as death of a spouse or divorce are closely related to relapses and aggravations of respiratory disease pointing to a link between psychological factors and chronic pulmonary disease. Patients with chronic obstructive pulmonary disease (COPD cannot cope adequately with everyday needs. This inadequacy may lead to heightened anxiety and depression, which in turn may worsen the everyday inadequacy. It has been reported that this is probably a factor that leads bronchial asthma (BA and COPD patients to frequent hospital admissions, lower treatment adherence and even intensive care unit hospitalizations.Study objectives: to assess anxiety and depressive symptoms among a sample of Egyptian patients with chronic obstructive pulmonary disease (COPD, bronchial asthma (BA, and diffuse parenchymatous lung diseases (DPLD.Subjects & methods: A cross-sectional study conducted over a one year period on a sample of 258 Egyptian patients with chronic breathing disorders who attending the outpatient chest clinic at Al-Zahraa University Hospital. All patients diagnosed as having COPD, BA or DPLD were enrolled into the study. Anxiety and depression were assessed by using the Hospital anxiety and depression scale (HADS and the Beck Depression Inventory (BDI.Results: Psychiatric disorders were most prevalent among DPLD cases (80.0%, followed by COPD patients (74.0%, while BA group showed the least reported cases (38.8% with psychiatric disorders, with a statistically significant difference among the studied groups (P-value < 0.05.Depression was prevalent among 60.1% (155/258 of the studied cases, of them 23.2% had severe depression, followed by 38.7 % had moderate depression and 38.1% had mild depression. DPLD cases had the highest proportion of severe depression (31.8, COPD cases had the highest proportion of moderate depression (52.4 and BA group had the highest proportion of mild depression (68.4%, with a statistically

  1. Elastic Deformation Properties of Implanted Endobronchial Wire Stents in Benign and Malignant Bronchial Disease: A Radiographic In Vivo Evaluation

    International Nuclear Information System (INIS)

    Purpose: To evaluate the long-term mechanical behavior in vivo of expandable endobronchial wire stents, we imaged three different prostheses in the treatment of tracheobronchial disease. Methods: Six patients with bronchial stenoses (three benign, three malignant) underwent insertion of metallic stents. Two self-expandable Wallstents, two balloon-expandable tantalum Strecker stents and two self-expandable nitinol Accuflex stents were used. Measurements of deformation properties were performed during voluntary cough by means of fluoroscopy, at 1 month and 7-10 months after implantation. The procedures were videotaped, their images digitized and the narrowing of stent diameters calculated at intervals of 20 msec. Results: After stent implantation all patients improved with respect to ventilatory function. Radial stent narrowing during cough reached 53% (Wallstent), 59% (tantalum Strecker stent), and 52% (nitinol Accuflex stent) of the relaxed post-implantation diameter. Stent compression was more marked in benign compared with malignant stenoses. In the long term permanent deformation occurred with the tantalum Strecker stents; the other stents were unchanged. Conclusion: Endobronchial wire stents can be helpful in the treatment of major airway collapse and obstructing bronchial lesions. However, evidence of material fatigue as a possible effect of exposure to recurrent mechanical stress on the flexible mesh tube may limit their long-term use. This seems to be predominantly important in benign bronchial collapse

  2. Bronchi, Bronchial Tree, & Lungs

    Science.gov (United States)

    ... specific Modules Resources Archived Modules Updates Bronchi, Bronchial Tree, & Lungs Bronchi and Bronchial Tree In the mediastinum , at the level of the ... trachea. As the branching continues through the bronchial tree, the amount of hyaline cartilage in the walls ...

  3. Bronchial stents

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    Ibrahim Emad

    2006-01-01

    Full Text Available Bronchial stents are mostly used as a Palliative relief of symptoms often caused by airway obstruction, It is also used for sealing of stump fistulas after pneumonectomy and dehiscence after bronchoplastic operations. Advances in airway prosthetics have provided a variety of silicone stents, expandable metal stents, and pneumatic dilators, enabling the correction of increasingly complex anatomical problems. Several series have been published describing the application and results of these techniques. This manuscript reviews the historical development of stents, types, indication, outcome, and complications. Alternative therapies for tracheobronchial stenting were also reviewed

  4. Symptoms, physical findings and bronchial hypersensitivity in patients with bronchial asthma and normal spirometry

    OpenAIRE

    Aćimović Slobodan; Plavec Goran; Tomić Ilija; Karličić Vukoica; Aćimović Svetlana; Vuković Jelena; Lončarević Olivera

    2009-01-01

    Background/Aim. The diagnosis of bronchial asthma, a chronic inflammatory disease of the respiratory tract, is made on the basis of anamnesis, pathologic auscultatory findings of the lungs, lung function disturbances, skin tests, as well as the basic indices of immunologic condition in bronchial trunk. The aim of the study was to find out correlation of objective indices of the disease and than relation with the symptoms in the patients with bronchial asthma. Methods. The study included 60 yo...

  5. Perennial atopic rhinitis as an early stage of bronchial asthma.

    Science.gov (United States)

    Gniazdowski, R

    1979-01-01

    Etiologic factors and incidence of bronchial hyperreactivity as a 'stigma' of bronchial asthma were studied in 237 patients suffering from perennial atopic rhinitis. All pateints underwent detailed laryngologic and allergologic examiniation and pulmonary function tests at rest, after exercise, and after histamine inhalation. Most often the patients were sensitized tungal allergens. Bronchial hyperreactivity, typical of bronchial asthma, was observed in 48.52% of patients. Results were analysed statistically. It was concluded that early institution of causal therapy can cure the symptoms of rhinitis and prevent evolution of the disease into atopic bronchial asthma in patients already suffering from bronchial hyperreactivity. PMID:495074

  6. ASSESSMENT OF KNOWLEDGE AND ATTITUDE OF BRONCHIAL ASTHMA PATIENTS TOWARDS THEIR DISEASE

    OpenAIRE

    Gaude; Nicasia; Sindhury; Jyothi

    2015-01-01

    Asthma is chronic inflammatory, airway hyper-responsiveness, reversible disorder which occurs at any age, and requires special attention towards management of drug therapy. There is lack of patients awareness in having the complete knowledge about the disease, attitude towards disease management, medication adherence behavior and treatment outcomes. The objective of the present study was to assess the knowledge and attitude of the patients towards their disease in improving the ...

  7. Specific IgA and metalloproteinase activity in bronchial secretions from stable chronic obstructive pulmonary disease patients colonized by Haemophilus influenzae

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    Millares Laura

    2012-12-01

    Full Text Available Abstract Background Haemophilus influenzae is the most common colonizing bacteria of the bronchial tree in chronic obstructive pulmonary disease (COPD, and positive cultures for this potentially pathogenic microorganism (PPM has been associated with local inflammation changes that may influence the relationships between H. influenzae and the bronchial mucosa. Methods A cross-sectional analysis of stable COPD patients enrolled in the Phenotype and Course of Chronic Obstructive Pulmonary Disease (PAC-COPD Study, focusing on bronchial colonization by H. influenzae, was performed. Specific IgA against the PPM was measured by optical density, and metalloproteinase-9 (MMP-9 and tissue inhibitor of metalloproteinase-1 (TIMP-1 using ELISA in sputum samples. Levels in patients colonized by H. influenzae and non-colonized patients were compared. Results Sputum supernatant for the measurement of specific IgA against H. influenzae was available from 54 stable COPD patients, who showed levels of specific IgA significantly lower in colonized (n=21 than in non-colonized patients (n=33 (15 [4-37] versus 31 [10-75], p=0.033, Mann-Whitney U test. Proenzyme MMP-9 was measured in 44 patients, and it was higher in colonized (n=12, 1903 [1488-6699] ng/ml than in non-colonized patients (n=32, 639 [373-972] ng/ml (p Conclusions Clinically stable COPD patients colonized by H. influenzae had lower levels of specific IgA against the microorganism and higher values of the active form of MMP-9 in their sputum supernatant than non-colonized patients. Bronchial colonization by H. influenzae may cause structural changes in the extracellular matrix through a defective defense and the production of active metalloproteinases.

  8. Stepwise health surveillance for bronchial irritability syndrome in workers at risk of occupational respiratory disease

    NARCIS (Netherlands)

    W.K. Post (Wendel); K.M. Venables (Katherine); D. Ross (David); P. Cullinan (Paul); D. Heederik (Dick); A. Burdorf (Alex)

    1998-01-01

    textabstractOBJECTIVES: Questionnaires, lung function tests, and peak flow measurements are widely used in occupational health care to screen for subjects with respiratory disease. However, the diagnostic performance of these tests is often poor. Application of these te

  9. A Dynamic Bronchial Airway Gene Expression Signature of Chronic Obstructive Pulmonary Disease and Lung Function Impairment

    NARCIS (Netherlands)

    Steiling, Katrina; van den Berge, Maarten; Hijazi, Kahkeshan; Florido, Roberta; Campbell, Joshua; Liu, Gang; Xiao, Ji; Zhang, Xiaohui; Duclos, Grant; Drizik, Eduard; Si, Huiqing; Perdomo, Catalina; Dumont, Charles; Coxson, Harvey O.; Alekseyev, Yuriy O.; Sin, Don; Pare, Peter; Hogg, James C.; McWilliams, Annette; Hiemstra, Pieter S.; Sterk, Peter J.; Timens, Wim; Chang, Jeffrey T.; Sebastiani, Paola; O'Connor, George T.; Bild, Andrea H.; Postma, Dirkje S.; Lam, Stephen; Spira, Avrum; Lenburg, Marc E.

    2013-01-01

    Rationale Molecular phenotyping of chronic obstructive pulmonary disease (COPD) has been impeded in part by the difficulty in obtaining lung tissue samples from individuals with impaired lung function. Objectives: We sought to determine whether COPD-associated processes are reflected in gene express

  10. Relationship between the thickness of bronchial wall layers, emphysema score, and markers of remodeling in bronchoalveolar lavage fluid in patients with chronic obstructive pulmonary disease.

    Science.gov (United States)

    Górka, Karolina; Soja, Jerzy; Jakieła, Bogdan; Plutecka, Hanna; Gross-Sondej, Iwona; Ćmiel, Adam; Mikrut, Sławomir; Łoboda, Piotr; Andrychiewicz, Anna; Jurek, Paulina; Sładek, Krzysztof

    2016-06-30

    INTRODUCTION    Airway remodeling plays an important role in the development of chronic obstructive pulmonary disease (COPD). Imaging methods, such as computed tomography (CT) and endobronchial ultrasound (EBUS), may be useful in the assessment of structural alterations in the lungs. OBJECTIVES    The aim of this study was to evaluate a relationship between the severity of emphysema assessed by chest CT, the thickness of bronchial wall layers measured by EBUS, and the markers of remodeling in bronchoalveolar lavage fluid (BALF) in patients with COPD. PATIENTS AND METHODS    The study included 33 patients with COPD who underwent pulmonary function tests, emphysema score assessment by chest CT, as well as bronchofiberoscopy with EBUS in order to measure the total bronchial wall thickness and, separately, layers L1, L2, and L3-5. Selected remodeling (matrix metalloproteinase 9 [MMP-9], tissue inhibitor of metalloproteinase 1, transforming growth factor β1 [TGF-β1]) and inflammatory markers (neutrophil elastase, eosinophil cationic protein) were measured in BALF samples using an enzyme-linked immunosorbent assay. RESULTS    MMP-9 levels in BALF were significantly higher in patients with very severe bronchial obstruction than in those with moderate and mild bronchial obstruction (P = 0.02), and showed a negative correlation with forced expiratory volume in 1 second (r = -0.538, P = 0.002). The thickness of L1 and L2, which histologically correspond to the mucosa, submucosa, and smooth muscle, demonstrated a positive correlation with TGF-β1 levels in BALF (r = 0.366, P = 0.046 and r = 0.425, P = 0.02) and the thickness of L1 showed a negative association with neutrophil elastase levels (r = -0.508, P = 0.004). There was no significant correlation between the analyzed markers in BALF and the emphysema score. CONCLUSIONS    Significant correlations of TGF-β1 and elastase with the thickness of bronchial wall layers, and of MMP-9 with the severity of

  11. Physiotherapy and bronchial mucus transport

    NARCIS (Netherlands)

    van der Schans, CP; Postma, DS; Koeter, GH; Rubin, BK

    1999-01-01

    Cough and expectoration of mucus are the best-known symptoms in patients with pulmonary disease, The most applied intervention for these symptoms is the use of chest physiotherapy to increase bronchial mucus transport and reduce retention of mucus in the airways, Chest physiotherapy interventions ca

  12. CFD modeling and image analysis of exhaled aerosols due to a growing bronchial tumor: Towards non-invasive diagnosis and treatment of respiratory obstructive diseases

    International Nuclear Information System (INIS)

    Diagnosis and prognosis of tumorigenesis are generally performed with CT, PET, or biopsy. Such methods are accurate, but have the limitations of high cost and posing additional health risks to patients. In this study, we introduce an alternative computer aided diagnostic tool that can locate malignant sites caused by tumorigenesis in a non-invasive and low-cost way. Our hypothesis is that exhaled aerosol distribution is unique to lung structure and is sensitive to airway structure variations. With appropriate approaches, it is possible to locate the disease site, determine the disease severity, and subsequently formulate a targeted drug delivery plan to treat the disease. This study numerically evaluated the feasibility of the proposed breath test in an image-based lung model with varying pathological stages of a bronchial squamous tumor. Large eddy simulations and a Lagrangian tracking approach were used to model respiratory airflows and aerosol dynamics. Respirations of tracer aerosols of 1 μm at a flow rate of 20 L/min were simulated, with the distributions of exhaled aerosols recorded on a filter at the mouth exit. Aerosol patterns were quantified with multiple analytical techniques such as concentration disparity, spatial scanning and fractal analysis. We demonstrated that a growing bronchial tumor induced notable variations in both the airflow and exhaled aerosol distribution. These variations became more apparent with increasing tumor severity. The exhaled aerosols exhibited distinctive pattern parameters such as spatial probability, fractal dimension, and multifractal spectrum. Results of this study show that morphometric measures of the exhaled aerosol pattern can be used to detect and monitor the pathological states of respiratory diseases in the upper airway. The proposed breath test also has the potential to locate the site of the disease, which is critical in developing a personalized, site-specific drug delivery protocol

  13. CFD Modeling and Image Analysis of Exhaled Aerosols due to a Growing Bronchial Tumor: towards Non-Invasive Diagnosis and Treatment of Respiratory Obstructive Diseases

    Energy Technology Data Exchange (ETDEWEB)

    Xi, Jinxiang; Kim, JongWon; Si, Xiuhua A.; Corley, Richard A.; Kabilan, Senthil; Wang, Shengyu

    2015-02-06

    Diagnosis and prognosis of tumorigenesis are generally performed with CT, PET, or biopsy. Such methods are accurate, but have the limitations of high cost and posing additional health risks to patients. In this study, we introduce an alternative computer aided diagnostic tool that can locate malignant sites caused by tumorigenesis in a non-invasive and low-cost way. Our hypothesis is that exhaled aerosol distribution is unique to lung structure and is sensitive to airway structure vari-ations. With appropriate approaches, it is possible to locate the disease site, determine the disease severity, and subsequently formulate a targeted drug delivery plan to treat the disease. This study numerically evaluated the feasibility of the proposed breath test in an image-based lung model with varying pathological stages of a bronchial squamous tumor. Large eddy simulations and a Lagran-gian tracking approach were used to model respiratory airflows and aerosol dynamics. Respira-tions of tracer aerosols of 1 µm at a flow rate of 20 L/min were simulated, with the distributions of exhaled aerosols recorded on a filter at the mouth exit. Aerosol patterns were quantified with multiple analytical techniques such as concentration disparity, spatial scanning and fractal analysis. We demonstrated that a growing bronchial tumor induced notable variations in both the airflow and exhaled aerosol distribution. These variations became more apparent with increasing tumor severity. The exhaled aerosols exhibited distinctive pattern parameters such as spatial probability, fractal dimension, and multifractal spectrum. Results of this study show that morphometric measures of the exhaled aerosol pattern can be used to detect and monitor the pathological states of respiratory diseases in the upper airway. The proposed breath test also has the potential to locate the site of the disease, which is critical in developing a personalized, site-specific drug de-livery protocol.

  14. Estudo da hiper-responsividade brônquica em pacientes portadores de refluxo gastroesofágico Bronchial hyperreactivity in patients with gastroesophageal reflux disease

    Directory of Open Access Journals (Sweden)

    Mônica Silveira Lapa

    2005-08-01

    Full Text Available OBJETIVO: O intuito deste trabalho foi evidenciar a existência desse reflexo vagal, através de uma broncoprovocação, em pacientes portadores de refluxo gastroesofágico. MÉTODOS: Onze pacientes com endoscopia sem evidências de refluxo gastroesofágico ou hérnia hiatal (grupo controle e dez pacientes com hérnia hiatal ou refluxo gastroesofágico foram submetidos à broncoprovocação com carbacol. RESULTADOS: O teste foi positivo em 5 dos pacientes com hérnia hiatal ou refluxo gastroesofágico (50%, e em 3 do grupo controle (27% (p = 0,64. CONCLUSÃO: A hipótese de que as vias aéreas de pacientes com refluxo gastroesofágico sem sintomas asmatiformes anteriores possam ser mais responsivas do que as de pacientes sem refluxo gastroesofágico permanece não comprovada.OBJECTIVE: To identify this vagal reflex using bronchial provocation tests in patients with gastroesophageal reflux disease. METHODS: The study group was composed of 10 patients presenting endoscopic evidence of hiatal hernia or gastroesophageal reflux disease, and the control group consisted of 11 patients presenting no evidence of either condition. All subjects were submitted to bronchial provocation with carbachol. RESULTS: The provocation test was positive in 5 (50% of the study group patients and 3 (27% of the control group patients (p = 0.64. CONCLUSION: The hypothesis that the airways of patients with gastroesophageal reflux disease (and no history of asthma-like respiratory symptoms might be more responsive than those of individuals without the disease remains unproven.

  15. Recent developments regarding periostin in bronchial asthma

    OpenAIRE

    Kenji Izuhara; Hisako Matsumoto; Shoichiro Ohta; Junya Ono; Kazuhiko Arima; Masahiro Ogawa

    2015-01-01

    Although it is currently recognized that bronchial asthma is not a single disease but a syndrome, we have not yet made use of our new understanding of this heterogeneity as we treat asthma patients. To increase the efficacy of anti-asthma drugs and to decrease costs, it is important to stratify asthma patients into subgroups and to develop therapeutic strategies for each subgroup. Periostin has recently emerged as a biomarker for bronchial asthma, unique in that it is useful not in diagnosis ...

  16. The predictive value of bronchial histamine challenge in the diagnosis of bronchial asthma

    DEFF Research Database (Denmark)

    Madsen, F; Holstein-Rathlou, N H; Mosbech, H;

    1985-01-01

    A prospective survey aiming to study the predictive value of bronchial histamine challenge was performed on 151 patients with a forced expiratory volume1 (FEV1) above 60% of predicted. According to variations in peak expiratory flow rate (PEFR) and medical history the patients were classified...... was below 0.125 mg/ml the predictive value of a positive test was 1.00, but an increase in PC20 in the range from 4.00 to 16 mg/ml did not increase the predictive value of a negative test. In this study the prevalence of asthma was about 0.6. We therefore conclude that bronchial histamine challenge...... is a valuable test for detection and exclusion of bronchial asthma, when the prevalence of the disease is high. In populations with a lower frequency of bronchial asthma the diagnostic value of a positive bronchial challenge will be negligible....

  17. Primary Human Bronchial Epithelial Cells Grown from Explants

    OpenAIRE

    Yaghi, Asma; Zaman, Aisha; Dolovich, Myrna

    2010-01-01

    Human bronchial epithelial cells are needed for cell models of disease and to investigate the effect of excipients and pharmacologic agents on the function and structure of human epithelial cells. Here we describe in detail the method of growing bronchial epithelial cells from bronchial airway tissue that is harvested by the surgeon at the times of lung surgery (e.g. lung cancer or lung volume reduction surgery). With ethics approval and informed consent, the surgeon takes what is needed for ...

  18. Recent developments regarding periostin in bronchial asthma.

    Science.gov (United States)

    Izuhara, Kenji; Matsumoto, Hisako; Ohta, Shoichiro; Ono, Junya; Arima, Kazuhiko; Ogawa, Masahiro

    2015-09-01

    Although it is currently recognized that bronchial asthma is not a single disease but a syndrome, we have not yet made use of our new understanding of this heterogeneity as we treat asthma patients. To increase the efficacy of anti-asthma drugs and to decrease costs, it is important to stratify asthma patients into subgroups and to develop therapeutic strategies for each subgroup. Periostin has recently emerged as a biomarker for bronchial asthma, unique in that it is useful not in diagnosis but in categorizing asthma patients. We first found that periostin is a novel component of subepithelial fibrosis in bronchial asthma downstream of IL-13 signals. Thereafter, it was shown that periostin can be a surrogate biomarker of type 2 immune responses, the basis of the notion that a detection system of serum periostin is potentially a companion diagnostic for type 2 antagonists. Furthermore, we have recently shown that serum periostin can predict resistance or hyporesponsiveness to inhaled corticosteroids, based on its contribution to tissue remodeling or fibrosis in bronchial asthma. Thus, serum periostin has two characteristics as a biomarker for bronchial asthma: it is both a surrogate biomarker of type 2 immune responses and a biomarker reflecting tissue remodeling or fibrosis. We can take advantage of these characteristics to develop stratified medicine in bronchial asthma. PMID:26344077

  19. Recent developments regarding periostin in bronchial asthma

    Directory of Open Access Journals (Sweden)

    Kenji Izuhara

    2015-09-01

    Full Text Available Although it is currently recognized that bronchial asthma is not a single disease but a syndrome, we have not yet made use of our new understanding of this heterogeneity as we treat asthma patients. To increase the efficacy of anti-asthma drugs and to decrease costs, it is important to stratify asthma patients into subgroups and to develop therapeutic strategies for each subgroup. Periostin has recently emerged as a biomarker for bronchial asthma, unique in that it is useful not in diagnosis but in categorizing asthma patients. We first found that periostin is a novel component of subepithelial fibrosis in bronchial asthma downstream of IL-13 signals. Thereafter, it was shown that periostin can be a surrogate biomarker of type 2 immune responses, the basis of the notion that a detection system of serum periostin is potentially a companion diagnostic for type 2 antagonists. Furthermore, we have recently shown that serum periostin can predict resistance or hyporesponsiveness to inhaled corticosteroids, based on its contribution to tissue remodeling or fibrosis in bronchial asthma. Thus, serum periostin has two characteristics as a biomarker for bronchial asthma: it is both a surrogate biomarker of type 2 immune responses and a biomarker reflecting tissue remodeling or fibrosis. We can take advantage of these characteristics to develop stratified medicine in bronchial asthma.

  20. ETIOLOGICAL STRUCTURE OF VIRAL RESPIRATORY DISEASES IN ADULT PATIENTS WITH BRONCHIAL ASTHMA EXACERBATION AND CHRONICAL BRONCHIOLITIS OBLITERANS

    Directory of Open Access Journals (Sweden)

    V. Z. Krivitskaya

    2015-01-01

    Full Text Available Exacerbation of bronchial asthma (BA and common deterioration of health during chronic bronchiolitis obliterans (ChBO are associated with viral infections in adults in 64 and 83% respectively. Mixed virus-viral associations were shown in 21–25% of cases. Respiratory syncytial infections were diagnosed with the highest frequency (50% in patients with BA. Influenza A(H1N1pdm09 and adenoviral infections dominated in persons with ChBO in 50 and 42% of cases, respectively. Response of virus-specific IgG in patients with BA and ChBO indicates the acute course of influenza A(H1N1pdm09 (63% of seroconversions. There were no reactions of IgG which is specific to respiratory syncytial virus in 75% of cases and to adenovirus in 83% of cases, that is the risk factor for occurrence of latent/persistent infection. Presence of structural components of respiratory syncytial virus in the upper respiratory tract had been revealed in three patients with asthma within at least 21–28 days. Respiratory syncytial viral infections and pandemic influenza A(H1N1 pdm09 in patients with BA and ChBO are characterized by the presence of an allergic component, which is indicated by the high levels of virus-specific IgE in blood. An adenoviral infection, in contrast, has no such peculiarity. 

  1. [Bronchial asthma pathogenesis and genetic prognosis development].

    Science.gov (United States)

    Balmasova, I P; Sepiashvili, R I; Sepiashvili, Ia R; Malova, E S

    2014-01-01

    The review is dedicated to an actual problem--genetic prognosis of risk of bronchial asthma development that is quite a complex aspect of studies from a methodological viewpoint. Bronchial asthma--heterogeneous disease by both etiology and clinical characteristics. At the same time genetic prognosis is based on the unity of pathogenetic mechanisms of development, though in immunological reactions that are the base of this disease, alternative variants are possible. The aim of this review is carrying out parallels between modern achievements in the field of deciphering trigger mechanisms of bronchial asthma pathogenesis and object of genetic studies based on these mechanisms. Among the examined conceptions--role of epithelial tissue in trigger mechanisms of bronchial asthma, variants of key role of immune system cells, first of all, T-helpers of various types for further development of inflammatory-effector reactions with damage characteristic for this disease. Compliance of contemporary approaches of genetic studies and novel concepts of bronchial asthma pathogenesis is shown.

  2. [Value of Fractional Exhaled Nitric Oxide after Using a Beta-2 Bronchodilator in the Differential Diagnosis of Bronchial Asthma and Chronic Obstructive Pulmonary Disease].

    Science.gov (United States)

    Ura, Midori; Tanaka, Hitomi; Takahashi, Kaori; Yamazaki, Haruna; Fujimoto, Keisaku

    2016-02-01

    It has been established that an increase in fractional exhaled nitric oxide (FeNO) is one of the indicators of bronchial asthma (BA) in clinical settings. However, the differential diagnosis of BA and chronic obstructive pulmonary disease (COPD) is difficult due to pathological similarities. Therefore, to determine if FeNO may be utilized in the differential diagnosis of BA and COPD, we compared FeNO values before and after inhalation of a short-acting beta-2 agonist (SABA). There were 3 groups of subjects recruited to this study: (1) 23 normal healthy controls, (2) 36 patients with BA, and (3) 13 patients with COPD. We measured FeNO, forced vital capacity, forced expiratory volume in 1 second (FEV1), and FEV1%, calculated using spirometry. Then, after the subjects inhaled the SABA, we measured these data after 10 and 30 minutes. Here we found that after inhalation of a SABA, 8 cases in the BA group who showed reversibility of airway obstruction demonstrated significantly increased FeNO values compared to the BA patients with non-reversible airway obstruction, those with COPD, and healthy subjects. This finding may be because the obstructed pulmonary peripheral airway was expanded by inhaling a SABA, and nitric oxide, which had been produced in the peripheral airway, was then exhaled. These results suggest the possibility that FeNO may be utilized in the differential diagnosis of BA and COPD. PMID:27311275

  3. Bronchial and pulmonary scintigraphy with radioactively marked aerosols

    International Nuclear Information System (INIS)

    In 97 patients with bronchitis, bronchial asthma, tuberculosis, sarcoidosis, pneumoconiosis, or tumors the mucociliary clearance and/or deposit pattern after inhalation of radioactively marked aerosols (1 mCi 99m Tc sulfur colloid) was studied. Normal values of the mucociliary 30 min. clearance for the central bronchial/lung periphery are 21%/15%. There was a decreased clearance with bronchitis (11/8%), bronchial asthma, emphysema, tuberculosis, sarcoidosis, trachiobronchial amyloidosis, pleural scarring or interstitial pneumona. Increased clearance (29/19%) was shown with pneumoconiosis. The correlation of deposit pattern and disease, for example, bronchitis, bronchial asthma, bullous emphysema, pleural scarring, partial lung resection, bronchopneumonia, or bronchial restriction, is described. In comparison of aerosol scintigraphy to perfusion scintigraphy and ventilation with gaseous xenon, the aerosol scintigraphy is superior to xenon for certain indications. The aerosol particles, which are larger in comparison to xenon, settle easier by obstructions or flow variations and thereby give better clinical indications of regional differences. (orig.)

  4. Scoliosis and Bronchial Obstruction

    Directory of Open Access Journals (Sweden)

    Mehdi Qiabi

    2015-01-01

    Full Text Available Severe scoliosis may have a significant effect on respiratory function. The effect is most often restrictive due to severe anatomical distortion of the chest, leading to reduced lung volumes, limited diaphragmatic excursion and chest wall muscle inefficiency. Bronchial compression by the deformed spine may also occur but is more unusual. Management options include a conservative approach using bracing and physiotherapy in mild cases, as well as surgical correction of the scoliosis in more severe cases. Bronchial stenting has also been used successfully as an alternative to surgical correction, and in cases in which spinal surgery was either unsuccessful or not feasible. The authors present a case involving a 52-year-old woman who exhibited symptomatic compression of the bronchus intermedius by severe residual scoliosis despite previous corrective surgery. She was treated with an indwelling bronchial stent.

  5. Bronchial secretion concentrations of tobramycin.

    Science.gov (United States)

    Alexander, M R; Schoell, J; Hicklin, G; Kasik, J E; Coleman, D

    1982-02-01

    The mean concentrations of tobramycin in bronchial secretions from patients with pneumonia were almost two times greater than secretions from patients free of lung infection. Mean tobramycin bronchial secretion to serum concentration ratios also were higher when obtained from infected lungs (0.66 versus 0.17) These data suggest that lung infection enhances the concentrations of tobramycin in bronchial secretions. PMID:7065524

  6. Rehabilitace u pacientů s astma bronchiale

    OpenAIRE

    Novák, Lukáš

    2011-01-01

    The topic of my thesis is the recovery of patients suffering from bronchial asthma. Asthma Bronchial (next asthma) is one of the most frequented civilisation diseases the prevalence of which has been increasing dramatically in the last 20 years. It is a chronic inflammatory disease of respiratory tract, which may appear at any age. The four major symptoms of asthma are whistling breath, shortness of breath, cough and tightness in the chest. Physiotherapy enables considerably a complex treatme...

  7. Iterative reconstruction technique vs filter back projection: utility for quantitative bronchial assessment on low-dose thin-section MDCT in patients with/without chronic obstructive pulmonary disease

    Energy Technology Data Exchange (ETDEWEB)

    Koyama, Hisanobu; Seki, Shinichiro; Sugimura, Kazuro [Kobe University Graduate School of Medicine, Division of Radiology, Department of Radiology, Kobe, Hyogo (Japan); Ohno, Yoshiharu; Nishio, Mizuho; Matsumoto, Sumiaki; Yoshikawa, Takeshi [Kobe University Graduate School of Medicine, Advanced Biomedical Imaging Research Centre, Kobe (Japan); Kobe University Graduate School of Medicine, Division of Functional and Diagnostic Imaging Research, Department of Radiology, Kobe (Japan); Sugihara, Naoki [Toshiba Medical Systems Corporation, Ohtawara, Tochigi (Japan)

    2014-08-15

    The aim of this study was to evaluate the utility of the iterative reconstruction (IR) technique for quantitative bronchial assessment during low-dose computed tomography (CT) as a substitute for standard-dose CT in patients with/without chronic obstructive pulmonary disease. Fifty patients (mean age, 69.2; mean % predicted FEV1, 79.4) underwent standard-dose CT (150mAs) and low-dose CT (25mAs). Except for tube current, the imaging parameters were identical for both protocols. Standard-dose CT was reconstructed using filtered back-projection (FBP), and low-dose CT was reconstructed using IR and FBP. For quantitative bronchial assessment, the wall area percentage (WA%) of the sub-segmental bronchi and the airway luminal volume percentage (LV%) from the main bronchus to the peripheral bronchi were acquired in each dataset. The correlation and agreement of WA% and LV% between standard-dose CT and both low-dose CTs were statistically evaluated. WA% and LV% between standard-dose CT and both low-dose CTs were significant correlated (r > 0.77, p < 0.00001); however, only the LV% agreement between SD-CT and low-dose CT reconstructed with IR was moderate (concordance correlation coefficient = 0.93); the other agreement was poor (concordance correlation coefficient <0.90). Quantitative bronchial assessment via low-dose CT has potential as a substitute for standard-dose CT by using IR and airway luminal volumetry techniques. circle Quantitative bronchial assessment of COPD using low-dose CT is possible. (orig.)

  8. Iterative reconstruction technique vs filter back projection: utility for quantitative bronchial assessment on low-dose thin-section MDCT in patients with/without chronic obstructive pulmonary disease

    International Nuclear Information System (INIS)

    The aim of this study was to evaluate the utility of the iterative reconstruction (IR) technique for quantitative bronchial assessment during low-dose computed tomography (CT) as a substitute for standard-dose CT in patients with/without chronic obstructive pulmonary disease. Fifty patients (mean age, 69.2; mean % predicted FEV1, 79.4) underwent standard-dose CT (150mAs) and low-dose CT (25mAs). Except for tube current, the imaging parameters were identical for both protocols. Standard-dose CT was reconstructed using filtered back-projection (FBP), and low-dose CT was reconstructed using IR and FBP. For quantitative bronchial assessment, the wall area percentage (WA%) of the sub-segmental bronchi and the airway luminal volume percentage (LV%) from the main bronchus to the peripheral bronchi were acquired in each dataset. The correlation and agreement of WA% and LV% between standard-dose CT and both low-dose CTs were statistically evaluated. WA% and LV% between standard-dose CT and both low-dose CTs were significant correlated (r > 0.77, p < 0.00001); however, only the LV% agreement between SD-CT and low-dose CT reconstructed with IR was moderate (concordance correlation coefficient = 0.93); the other agreement was poor (concordance correlation coefficient <0.90). Quantitative bronchial assessment via low-dose CT has potential as a substitute for standard-dose CT by using IR and airway luminal volumetry techniques. circle Quantitative bronchial assessment of COPD using low-dose CT is possible. (orig.)

  9. 'Heavier the interval than the consummation': bronchial disease in Seán Ó Ríordáin's diaries.

    Science.gov (United States)

    Breathnach, Ciara

    2014-06-01

    Narratives of the experience of pulmonary tuberculosis (TB) are relatively rare in the Irish context. A scourge of the early twentieth century, TB was as much a social as a physically debilitating disease that rendered sufferers silent about their experience. Thus, the personal diaries and letters of Irish poet, Seán Ó Ríordáin, (1916-1977) are rare. This article presents translations of his personal papers in a historico-medical context to chronicle Ó Ríordáin's experience of a life marred by respiratory disease. Familiar to generations of schoolchildren are his imaginative poems, whose lively metre punctuated the Irish language curriculum from primary through to secondary schooling; for most they leave an indelible mark. Such buoyant poems however belie the reality of his existence, lived in the shadow of chronic illness, and punctuated with despair over his condition and anxiety about the periods of extended sick leave his illness necessitated. Although despair dominated his diaries and he routinely begged God, Mary, the Saints and the devil for death, they were also the locus where his creativity developed. In his diaries, caricatures of friends and sketches of everyday things nestle among the first lines of some of his most influential poems and quotes from distinguished philosophers and writers. Evocative and tragic, his diaries offer a unique prism to the experience of respiratory disease in Ireland. PMID:24002457

  10. Scoliosis and bronchial obstruction

    OpenAIRE

    Mehdi Qiabi; Karine Chagnon; Alain Beaupré; Julian Hercun; George Rakovich

    2015-01-01

    Severe scoliosis may have a significant effect on respiratory function. The effect is most often restrictive due to severe anatomical distortion of the chest, leading to reduced lung volumes, limited diaphragmatic excursion and chest wall muscle inefficiency. Bronchial compression by the deformed spine may also occur but is more unusual. Management options include a conservative approach using bracing and physiotherapy in mild cases, as well as surgical correction of the scoliosis in more sev...

  11. In vitro bioelectric properties of bronchial epithelium from transplanted lungs in recipients with cystic fibrosis.

    OpenAIRE

    Tsang, V. T.; Alton, E. W.; Hodson, M E; Yacoub, M

    1993-01-01

    BACKGROUND--Bronchial epithelial function after heart-lung transplantation (HLT) for cystic fibrosis (CF) may be affected by the original disease as well as other factors such as prolonged organ ischaemic time, the interruption of bronchial arterial and lymphatic supply, infection, rejection, and cyclosporin. In vitro measurement of the bioelectric properties of the bronchial mucosal lining may be an effective means of characterising the mucosal function of the lung allografts in response to ...

  12. [Bronchial mucoepidermoid carcinoma].

    Science.gov (United States)

    Bregante, J I; Rituerto, B; Font de Mora, F; Alonso, F; Andreu, M J; Figuerola, J; Mulet, J F

    1998-07-01

    We submit the case of a child afflicted with a mucoepidermoid bronchial tumor. The patient is a boy, aged seven, who after undergoing antibiotic treatment for six weeks because of a fever and atelectasia-condensation in the right lower lobe showed no signs of clinical improvement and was sent to our department to undergo further study and treatment. A bronchoscopy performed shows a polypoid mass that partially blocks the main bronchial tube a few milimiters under the access to the right upper lobe. A biopsy is carried out and the anatomopathological test shows there is a low degree epidermoid carcinoma. We decide to perform a lobectomy which for the tumor location and the lung condition has to be medium and lower right. We proceed to remove the adenopaty of hilium not affected by the tumor. The postoperative period develops without incidents. A check-up bronchoscopy performed three months later shows two polypoid masses in the right bronchial tube which, once a biopsy is performed, proved to be granulation tissue. Twelve months after undergoing surgery, the patient's condition is good, there is no evidence of tumor relapse and the breathing capacity is adequate, though there is an obstructive restrictive pattern in the espirometry. Even taking into consideration that lung tumors are extremely unusual, the epidermoid carcinoma is the one which most frequently occurs. The tumor's low malignancy is a sign that points to a good prognosis. Performing conservative surgery by means of bronchoplasty should be taken into account so as to keep the sequelae on the lung condition to a minimum, even though in this case the tumor location made it impossible. PMID:12602035

  13. Symptoms, physical findings and bronchial hypersensitivity in patients with bronchial asthma and normal spirometry

    Directory of Open Access Journals (Sweden)

    Aćimović Slobodan

    2009-01-01

    Full Text Available Background/Aim. The diagnosis of bronchial asthma, a chronic inflammatory disease of the respiratory tract, is made on the basis of anamnesis, pathologic auscultatory findings of the lungs, lung function disturbances, skin tests, as well as the basic indices of immunologic condition in bronchial trunk. The aim of the study was to find out correlation of objective indices of the disease and than relation with the symptoms in the patients with bronchial asthma. Methods. The study included 60 young male non smokers with long lasting symptoms of bronchial asthma including shortness of breath, wheezing, hard breathing, nonproductive or productive cough, weakness and night hard breathing. There were no symptoms of respiratory infection over the past two months and lung radiography and spirometry were normal. Based on the results of nonspecific bronchoprovocative test two groups of the patients were formed, group I (n = 30 with positive histamine test (average value of the inhaled histamine concentration with FEV1 drop by 20% in regard with the initial value (PC20 = 2.99 ± 0.51 mg/ml of histamine and group II (n = 30 with negative histamine test (PC20(a = 14.58 ± 6.34 mg/ml of histamine. Results. The obtained spirometry results revealed a statistically significant difference in values of FEV1 between groups: I group - FEV1 = 93.2%; II group - FEV1 = 101.8%; (p < 0.05, Wilcoxon test, although all the FEV1 values were normal. Regarding the presence of the most common symptoms there was not statistically significant difference between the groups (p > 0. 05, chisquare test. Pathologic auscultatory lung findings were found in 73.4% of the patients in the group I and 27.5% of the patients in the group II. There was statistically significant difference (p < 0.05, chi-squared test. A positive correlation between the degree of hypersensitivity and lung physical findings was confirmed (p < 0.05 Spearman's rho, but there was no correlation with FEV1 values

  14. Reflexology and bronchial asthma

    DEFF Research Database (Denmark)

    Brygge, T; Heinig, J H; Collins, P;

    2001-01-01

    Many asthma patients seek alternative or adjunctive therapies. One such modality is reflexology, whereby finger pressure is applied to certain parts of the body. The aim of the study was to examine the popular claim that reflexology treatment benefits bronchial asthma. Ten weeks of active...... or simulated (placebo) reflexology given by an experienced reflexologist, were compared in an otherwise blind, controlled trial of 20+20 outpatients with asthma. Objective lung function tests (peak flow morning and evening, and weekly spirometry at the clinic) did not change. Subjective scores (describing...... diaries was carried out. It was accompanied by a significant pattern compatible with subconscious unblinding, in that patients tended to guess which treatment they had been receiving. No evidence was found that reflexology has a specific effect on asthma beyond placebo influence....

  15. Bronchial reactivity in patients with recent pulmonary sarcoidosis.

    OpenAIRE

    Olafsson, M; Simonsson, B. G.; Hansson, S B

    1985-01-01

    Non-specific bronchial reactivity was assessed in 17 consecutive non-smoking and non-steroid treated patients with recently diagnosed pulmonary sarcoidosis, 11 with stage I disease and six with stage II disease. Bronchial reactivity was measured by recording the FEV1 after increasing doses of methacholine. Three subjects with asthma were hyperreactive. The 14 subjects with no asthma had a mean FEV1 of 96% predicted. Only one was hyperreactive, with a fall in FEV1 of over 15% after 0.1% methac...

  16. Neurotrophins in bronchial asthma

    Directory of Open Access Journals (Sweden)

    Renz Harald

    2001-07-01

    Full Text Available Abstract Allergic bronchial asthma (BA is characterized by chronic airway inflammation, development of airway hyperreactivity and recurrent reversible airway obstruction. T-helper 2 cells and their products have been shown to play an important role in this process. In contrast, the mechanisms by which immune cells interact with the cells residing in lung and airways, such as neurons, epithelial or smooth muscle cells, still remains uncertain. Sensory and motor neurons innervating the lung exhibit a great degree of functional plasticity in BA defined as 'neuronal plasticity'. These neurons control development of airway hyperresponsiveness and acute inflammatory responses, resulting in the concept of 'neurogenic inflammation'. Such quantitative and/or qualitative changes in neuronal functions are mediated to a great extent by a family of cytokines, the neurotrophins, which in turn are produced by activated immune cells, among others in BA. We have therefore developed the concept that neurotrophins such as nerve growth factor and brain-derived neurotrophic factor link pathogenic events in BA to dysfunctions of the immune and nervous system.

  17. Connective Tissue Growth Factor Expression in Human Bronchial Epithelial Cells

    Institute of Scientific and Technical Information of China (English)

    Amrita DOSANJH

    2006-01-01

    Connective tissue growth factor (CTGF) is a cysteine-rich protein that promotes extracellular matrix deposition. CTGF is selectively induced by transforming growth factor β and des-Arg kallidin in lung fibroblasts and increases steady-state mRNA levels of α type I collagen, 5α-integrin and fibronectin in fibroblasts. Bronchial epithelial cells have been proposed to functionally interact with lung fibroblasts. We therefore investigated if bronchial epithelial cells are able to synthesize CTGF. Human bronchial epithelial cells were grown to subconfluence in standard growth media. Proliferating cells grown in small airway growth media were harvested following starvation for up to 24 h. Expression of CTGF transcripts was measured by PCR. Immunocytochemistry was also completed using a commercially available antibody.The cells expressed readily detectable CTGF transcripts. Starvation of these cells resulted in a quantitative decline of CTGF transcripts. Direct sequencing of the PCR product identified human CTGF. Immunocytochemistry confirmed intracellular CTGF in the cells and none in negative control cells. We conclude that bronchial epithelial cells could be a novel source of CTGF. Bronchial epithelial cell-derived CTGF could thus directly influence the deposition of collagen in certain fibrotic lung diseases.

  18. Lithium Attenuates TGF-β 1-Induced Fibroblasts to Myofibroblasts Transition in Bronchial Fibroblasts Derived from Asthmatic Patients

    OpenAIRE

    Marta Michalik; Katarzyna Anna Wójcik; Bogdan Jakieła; Katarzyna Szpak; Małgorzata Pierzchalska; Marek Sanak; Zbigniew Madeja; Jarosław Czyż

    2012-01-01

    Bronchial asthma is a chronic disorder accompanied by phenotypic transitions of bronchial epithelial cells, smooth muscle cells, and fibroblasts. Human bronchial fibroblasts (HBFs) derived from patients with diagnosed asthma display predestination towards TGF- β -induced phenotypic switches. Since the interference between TGF- β and GSK-3 β signaling contributes to pathophysiology of chronic lung diseases, we investigated the effect of lithium, a nonspecific GSK-3 β inhibitor, on TGF- β 1-ind...

  19. Duodenal-bronchial fistula: an unusual cause of shortness of breath and a productive cough

    Directory of Open Access Journals (Sweden)

    Cynthia Wong, BMBS

    2016-09-01

    Full Text Available Duodenal-bronchial fistulas are very uncommon, even among the already rare subgroup of abdominal-bronchial fistulas. We describe a case of a woman with Crohn's disease who presented with shortness of breath and a productive cough who was found to have a duodeanl bronchial fistula on computed tomography scan. We demonstrate with this case how these rare cases can lead to chronic lung aspirations and require multidisciplinary involvement.

  20. Duodenal-bronchial fistula: an unusual cause of shortness of breath and a productive cough.

    Science.gov (United States)

    Wong, Cynthia; Khan, Kalim; Byass, Oliver

    2016-09-01

    Duodenal-bronchial fistulas are very uncommon, even among the already rare subgroup of abdominal-bronchial fistulas. We describe a case of a woman with Crohn's disease who presented with shortness of breath and a productive cough who was found to have a duodeanl bronchial fistula on computed tomography scan. We demonstrate with this case how these rare cases can lead to chronic lung aspirations and require multidisciplinary involvement. PMID:27594936

  1. Information for the diagnosis of broncho-pulmonary diseases obtained by the combined use of perfusion lung scintigraphy and selective bronchial arteriography

    International Nuclear Information System (INIS)

    Our study is limited to perfusion lung scintigraphy and deals with the following in turn: - radioisotopes and their conditioning, - detection and recording equipment, - results and limits of the method. The tracers used here were radioiodine-labelled human albumin macro-aggregates and more especially technetium-labelled microspheres. Scintigraphy, which visualizes or charts the local distribution of radioactive indicators, is carried out with two kinds of apparatus, scanners and scintillation cameras. The information acquired may be accurately analysed at the same time owing to modern data processing systems. The interpretation of radiotracer lung transit curves made by several authors is not very useful for our work, consequently we have kept to the analysis of images obtained by the Anger camera or similar instruments. The Anger scintillation camera seems to be well suited to isotopic lung exploration providing morphological and functional data simultaneously. Perfusion lung scintigraphy, which reflects the quality of lung circulation at the capillary level, appears to be a valuable asset in the diagnostic approach to certain chest X-ray images. The technique is fast, painless, neat, harmless and almost never contra-indicated. However like other techniques it cannot solve every problem on its own. Under these conditions, we must admit, on weighing up the results of this study, that perfusion lung scintigraphy had to be followed in most cases by selective bronchial arteriography

  2. The frequency and importance of bronchial hyperreactivity in patients with allergic and non-allergic rhinitis.

    Science.gov (United States)

    Dziedziczko, A; Gniazdowski, R

    1977-01-01

    A trial was undertaken to evaluate the occurrence of bronchial hyperreactivity, typical of bronchial asthma, in 50 patients with hay fever (rhinitis allergica) and 45 patients with rhinitis vasomotorica nonallergica, as opposed to a group of healthy subjects and patients with bronchial asthma. All the patients were subjected to spirographic examinations at rest (VC, FEV1, ETT, SI), after exercise (PWC170), and after histamine inhalation. The authors believe that it is expedient to study bronchial hyperreactivity in patients with hay fever and rhinitis vasomotorica nonallergica in that it affords possibilities for the prevision of the conceivable unfavourable evolution of the disease towards the atopic or non-atopic bronchial asthma, as well as for the taking of adequate preventive and therapeutic measures. PMID:920144

  3. CHALLENGES IN BRONCHIAL CHALLENGE TESTING

    NARCIS (Netherlands)

    Lexmond, A. J.; Hagedoorn, P.; Frijlink, H. W.; de Boer, A. H.

    2013-01-01

    Background: In the adenosine 5'-monophosphate (AMP) bronchial challenge test, AMP is usually administered according to dosing protocols developed for histamine/methacholine. It has never been thoroughly investigated whether these protocols are suitable for AMP as well. Methods: The setup of the two-

  4. The difference of the regional pulmonary function after treatment between bronchial tuberculosis and bronchogenic carcinoma using positron emission tomography, N-13 gas and Tc-MAA

    International Nuclear Information System (INIS)

    The authors studied regional pulmonary function after treatment with bronchial plastic surgery for bronchial lesion from bronchial tuberculosis and with irradiation for bronchial lesion from carcinoma using positron emission tomography and Tc-MAA. Six patients with bronchial tuberculosis and 6 with bronchogenic carcinoma were examined. Two of the 6 bronchial tuberculosis patients were examined before and after surgery. In all 6 patients with bronchial tuberculosis, ventilation and alveolar volume were recovered with improvement of bronchial lesion. In 2 patients examined before and after surgery, lung function of the normal disease-free side became worse after surgery. This phenomenon was explained by the fact that the compensated pulmonary function of contra lateral lung due to decreased function of the diseased side returned to normal function with improvement of the diseased lung. On the other hand, the regional pulmonary function did not improve in 2 patients with main bronchial lesion from bronchogenic carcinoma, even if atelectasis of the diseased lung was improved by irradiation. However, the regional pulmonary function was improved with the recovery of bronchial obstruction in lobar atelectasis by carcinoma. The difference of the results depended on whether pulmonary blood flow disturbance existed or not. If pulmonary blood flow disturbance was severe, ventilatory function was not recovered due to the mechanism of maintaining the ventilation-perfusion equilibrium. (author)

  5. Primary human bronchial epithelial cells grown from explants.

    Science.gov (United States)

    Yaghi, Asma; Zaman, Aisha; Dolovich, Myrna

    2010-01-01

    Human bronchial epithelial cells are needed for cell models of disease and to investigate the effect of excipients and pharmacologic agents on the function and structure of human epithelial cells. Here we describe in detail the method of growing bronchial epithelial cells from bronchial airway tissue that is harvested by the surgeon at the times of lung surgery (e.g. lung cancer or lung volume reduction surgery). With ethics approval and informed consent, the surgeon takes what is needed for pathology and provides us with a bronchial portion that is remote from the diseased areas. The tissue is then used as a source of explants that can be used for growing primary bronchial epithelial cells in culture. Bronchial segments about 0.5-1cm long and open and minced into 2-3mm(3) pieces of tissue. The pieces are used as a source of primary cells. After coating 100mm culture plates for 1-2 hr with a combination of collagen (30 microg/ml), fibronectin (10 microg/ml), and BSA (10 microg/ml), the plates are scratched in 4-5 areas and tissue pieces are placed in the scratched areas, then culture medium (DMEM/Ham F-12 with additives) suitable for epithelial cell growth is added and plates are placed in an incubator at 37 degrees C in 5% CO(2) humidified air. The culture medium is changed every 3-4 days. The epithelial cells grow from the pieces forming about 1.5 cm diameter rings in 3-4 weeks. Explants can be re-used up to 6 times by moving them into new pre-coated plates. Cells are lifted using trypsin/EDTA, pooled, counted, and re-plated in T75 Cell Bind flasks to increase their numbers. T75 flasks seeded with 2-3 million cells grow to 80% confluence in 4 weeks. Expanded primary human epithelial cells can be cultured and allowed to differentiate on air-liquid interface. Methods described here provide an abundant source of human bronchial epithelial cells from freshly isolated tissues and allow for studying these cells as models of disease and for pharmacology and toxicology

  6. RATIONALE FOR A SPECIFIC THERAPY OF CYTOMEGALOVIRUS INFECTION IN CHILDREN WITH BRONCHIAL ASTHMA

    Directory of Open Access Journals (Sweden)

    E. N. Suprun

    2013-01-01

    Full Text Available Abstract. We propose a protocol of treatment in cases of bronchial asthma with cytomegalovirus (CMV persistence. This basic therapy is administered depending on the disease severity, according to the National Programme 2009. The treatment includes administration of human immunoglobulin, with dosage according on CMV antibodies titers. The study has revealed that such regimen of antibody administration based on the content of anti-CMV antibodies in bronchial asthma treatment stops active CMV replication in bronchial mucous membrane, alleviates clinical course of the disease, diminishes changes of immune system typical to children suffering from bronchial asthma and CMV reactivation, thus allowing to reduce the volume of basic therapy, along with maintaining control of asthma control.

  7. Accurate 3D quantification of the bronchial parameters in MDCT

    Science.gov (United States)

    Saragaglia, A.; Fetita, C.; Preteux, F.; Brillet, P. Y.; Grenier, P. A.

    2005-08-01

    The assessment of bronchial reactivity and wall remodeling in asthma plays a crucial role in better understanding such a disease and evaluating therapeutic responses. Today, multi-detector computed tomography (MDCT) makes it possible to perform an accurate estimation of bronchial parameters (lumen and wall areas) by allowing a quantitative analysis in a cross-section plane orthogonal to the bronchus axis. This paper provides the tools for such an analysis by developing a 3D investigation method which relies on 3D reconstruction of bronchial lumen and central axis computation. Cross-section images at bronchial locations interactively selected along the central axis are generated at appropriate spatial resolution. An automated approach is then developed for accurately segmenting the inner and outer bronchi contours on the cross-section images. It combines mathematical morphology operators, such as "connection cost", and energy-controlled propagation in order to overcome the difficulties raised by vessel adjacencies and wall irregularities. The segmentation accuracy was validated with respect to a 3D mathematically-modeled phantom of a pair bronchus-vessel which mimics the characteristics of real data in terms of gray-level distribution, caliber and orientation. When applying the developed quantification approach to such a model with calibers ranging from 3 to 10 mm diameter, the lumen area relative errors varied from 3.7% to 0.15%, while the bronchus area was estimated with a relative error less than 5.1%.

  8. Bronchial morphometry in smokers: comparison with healthy subjects by using 3D CT

    Energy Technology Data Exchange (ETDEWEB)

    Montaudon, Michel [Unite d' Imagerie Thoracique, CHU de Bordeaux, Pessac (France); Universite Bordeaux 2, Laboratoire de Physiologie Cellulaire Respiratoire, Bordeaux (France); INSERM, Bordeaux (France); Hopital Cardiologique, Unite d' Imagerie Thoracique, Pessac (France); Berger, Patrick; Marthan, Roger [Universite Bordeaux 2, Laboratoire de Physiologie Cellulaire Respiratoire, Bordeaux (France); INSERM, Bordeaux (France); Service d' Exploration Fonctionnelle Respiratoire, CHU de Bordeaux, Pessac (France); Lederlin, Mathieu [Unite d' Imagerie Thoracique, CHU de Bordeaux, Pessac (France); Tunon-de-Lara, Jose Manuel [Universite Bordeaux 2, Laboratoire de Physiologie Cellulaire Respiratoire, Bordeaux (France); INSERM, Bordeaux (France); Service des Maladies Respiratoires, CHU de Bordeaux, Pessac (France); Laurent, Francois [Unite d' Imagerie Thoracique, CHU de Bordeaux, Pessac (France); Universite Bordeaux 2, Laboratoire de Physiologie Cellulaire Respiratoire, Bordeaux (France); INSERM, Bordeaux (France)

    2009-06-15

    The assessment of airway dimensions in patients with airway disease by using computed tomography (CT) has been limited by the obliquity of bronchi, the ability to identify the bronchial generation, and the limited number of bronchial measurements. The aims of the present study were (i) to analyze cross-sectional bronchial dimensions after automatic orthogonal reconstruction of all visible bronchi on CT images, and (ii) to compare bronchial morphometry between smokers and nonsmokers. CT and pulmonary function tests were performed in 18 males separated into two groups: 9 nonsmokers and 9 smokers. Bronchial wall area (WA) and lumen area (LA) were assessed using dedicated 3D software able to provide accurate cross-sectional measurements of all visible bronchi on CT. WA/LA and WA/(WA+LA) ratios were computed and all parameters were compared between both groups. Smokers demonstrated greater WA, smaller LA, and consequently greater LA/WA and LA/(WA+LA) ratios than nonsmokers. These differences occurred downward starting at the fourth bronchial generation. 3D quantitative CT method is able to demonstrate significant changes in bronchial morphometry related to tobacco consumption. (orig.)

  9. Effects of hypothyroidism on bronchial reactivity in non-asthmatic subjects.

    OpenAIRE

    Wieshammer, S; Keck, F S; Schäuffelen, A C; von Beauvais, H; Seibold, H.; Hombach, V.

    1990-01-01

    The effect of hypothyroidism on non-specific bronchial reactivity was studied in 11 patients without pulmonary disease (mean age 40 (SD 13) years) who had had a total thyroidectomy and radioiodine treatment for thyroid cancer 41 (36) months before the study. All patients when mildly hyperthyroid while having long term thyroxine replacement treatment and once when hypothyroid two weeks after stopping triiodothyronine for the purpose of screening for metastases. Bronchial reactivity was assesse...

  10. Bronchial Anthracotic Change in South Khorasan Province (Iran), Emphasizing its Association with Tuberculosis

    OpenAIRE

    Sayyed Gholamreza Mortazavi-Moghaddam; Sayyed Alireza Saadatjoo

    2014-01-01

    Background: There are many reports on the association between anthracosis and tuberculosis. This study focuses on bronchial anthracosis and associated diseases in the province of South Khorasan-Iran. Methods: This case-series study is performed on patients referred to the Vali-e-Asre Hospital (South Khorasan-Iran) for bronchoscopic evaluations during the period of 2009-2012. Written informed consents were obtained prior to bronchoscopic evaluations. The criterion for diagnosis of bronchial...

  11. Oilseed rape and bronchial reactivity.

    OpenAIRE

    Soutar, A; Harker, C; Seaton, A.; Packe, G

    1995-01-01

    OBJECTIVES--To investigate atopy and changes in symptoms, peak flow rate, and bronchial reactivity in people complaining of symptoms during the oilseed rape flowering season. METHODS--37 people who had given positive answers to questions about the presence of symptoms in relation to the flowering season of oilseed rape and 24 controls with no such symptoms were studied, although not all took part in all parts of the study. All had been previously identified in a cross sectional survey of a ra...

  12. Treatment of bronchial ruptures by delayed surgery

    Institute of Scientific and Technical Information of China (English)

    1999-01-01

    Objective:To study the causes that resulted in delayed surgery for bronchial ruptures and the results.Methods:The cases with the bronchial ruptures by the delayed surgery last decade were retrospectively reviewed.The causes and unsatisfactory results were analysed.Results:The severe complications usually occurred after the delayed surgery and the results were not as satisfactory as those by early surgery.Conclusion:The bronchial ruptures ought to be operated in the early stage after being wounded.

  13. Main bronchial diverticula in the subcarinal region: Their relation to airflow limitations

    Energy Technology Data Exchange (ETDEWEB)

    Higuchi, Takeshi; Takahashi, Naoya; Shiotani, Motoi; Sato, Suguru; Ohta, Atsushi; Maeda, Haruo; Nakajima, Haruhiko; Itoh, Kazuhiko; Tsukada, Hiroki (Department of Radiology, Respiratory Medicine, Niigata City General Hospital, Niigata-city, Niigata-ken (Japan)), Email: higuchi@hosp.niigata.niigata.jp

    2012-02-15

    Background. To date, bronchial diverticula have generally been treated as a pathological condition associated with chronic obstructive pulmonary disease (COPD), although only a limited amount of published information is available on the relationship between bronchial diverticula as depicted by multidetector computed tomography (MDCT) and airflow limitations. Purpose. To evaluate the relationship between airflow limitations and main bronchial diverticula in the subcarinal region using spirometry and thin-section MDCT. Material and Methods. A total of 189 consecutive adult patients were retrospectively evaluated based on spirometry and thin-section MDCT of the chest. All examinations were performed at our institution between June and October 2008. The study group included 70 women and 119 men with a mean age of 65 years (range 19-86 years). The relationship between the FEV1% and bronchial diverticula in the subcarinal region was analyzed (Student's t-test). Results. The indications for conducting the examinations were pulmonary diseases (82 patients), cardiovascular diseases (22), extrapulmonary malignancies (74), and other conditions (11). A total of 84/189 (44.4%) patients showed bronchial diverticula, and the FEV{sub 1}% of 70/84 (83.3%) patients was above 70. The FEV{sub 1}% of patients with lesions ranged from 26.0 to 97.8 (mean 76.8), whereas the range was 28.1-94.4 (mean 73.7) in those without lesions. There was no significant association between the FEV{sub 1}% and the presence of subcarinal bronchial diverticula (P > 0.05). Conclusion. Our data demonstrate that thin-section chest CT commonly demonstrates main bronchial diverticula in the subcarinal region in patients without airflow limitations. We propose that the presence of a small number of tiny bronchial diverticula under the carina may not be a criterion for the diagnosis of COPD

  14. Main bronchial diverticula in the subcarinal region: Their relation to airflow limitations

    International Nuclear Information System (INIS)

    Background. To date, bronchial diverticula have generally been treated as a pathological condition associated with chronic obstructive pulmonary disease (COPD), although only a limited amount of published information is available on the relationship between bronchial diverticula as depicted by multidetector computed tomography (MDCT) and airflow limitations. Purpose. To evaluate the relationship between airflow limitations and main bronchial diverticula in the subcarinal region using spirometry and thin-section MDCT. Material and Methods. A total of 189 consecutive adult patients were retrospectively evaluated based on spirometry and thin-section MDCT of the chest. All examinations were performed at our institution between June and October 2008. The study group included 70 women and 119 men with a mean age of 65 years (range 19-86 years). The relationship between the FEV1% and bronchial diverticula in the subcarinal region was analyzed (Student's t-test). Results. The indications for conducting the examinations were pulmonary diseases (82 patients), cardiovascular diseases (22), extrapulmonary malignancies (74), and other conditions (11). A total of 84/189 (44.4%) patients showed bronchial diverticula, and the FEV1% of 70/84 (83.3%) patients was above 70. The FEV1% of patients with lesions ranged from 26.0 to 97.8 (mean 76.8), whereas the range was 28.1-94.4 (mean 73.7) in those without lesions. There was no significant association between the FEV1% and the presence of subcarinal bronchial diverticula (P > 0.05). Conclusion. Our data demonstrate that thin-section chest CT commonly demonstrates main bronchial diverticula in the subcarinal region in patients without airflow limitations. We propose that the presence of a small number of tiny bronchial diverticula under the carina may not be a criterion for the diagnosis of COPD

  15. Distal bronchial tubes damage in patients with rheumatoid arthritis

    Directory of Open Access Journals (Sweden)

    M V Sheyanov

    2009-12-01

    Full Text Available Objective. To study prevalence and clinical picture of distal parts of bronchial tree(bronchiolitis in pts with rheumatoid arthritis (RA. Material and methods. 104 nonsmoking pts with RA and 100 pts without RA and chronic diseases of respiratory apparatus were included. General clinical examination, spirometry, bodyplethysmography, examination of lung diffusion capacity (LDC and multispiralcomputed tomography (MSCT of lungs were performed. Results. Direct and indirect bronchiolitis signs were revealed with MSCT in 36 (35% ptswith RA and 1 pt of control group (p<0,01. Pts with signs of bronchiolitis complained of shortness of breath (69% of pts, cough (56%, phlegm discharge (56%, heavy breathing (25%. Obstructive lung ventilation disturbances were revealed in 19 (53% RA pts with bronchiolitis. Restrictive disturbances and LDC decrease were present in 3 (8% pts. High frequency of oligosymptomatic bronchiolitis course was found in RA pts. Bronchiolitis symptoms in RA pts coincided with signs of proximal bronchial tubes damage forming picture of diffuse damage of bronchial tree. Conclusion. Bronchiolitis is a prevalent variant of respiratory apparatus damage in pts with RA. Pts with MSCT signs of bronchiolitis often have cough, phlegm discharge, shortness of breath, heavy breathing. Lung ventilation disturbances of obstructive type are common but part of pts has normal lung functional measures or restriction. Oligosymptomatic forms of distal bronchial tubes damage are prevalent in RA pts. Lung MSCT is the main method of bronchiolitis diagnostics because bronchiolitis induces nonspecific clinical signs and RA pts have multilevel respiratory apparatus damage. MSCT reveals signs of distal bronchial tubes damage in 35% of RA pts.

  16. Effect of TRPV1 gene mutation on bronchial asthma in children before and after treatment.

    Science.gov (United States)

    Chen, Chuan-Liang; Li, Hong; Xing, Xiao-Hong; Guan, Hai-Shan; Zhang, Jian-Hua; Zhao, Jun-Wu

    2015-01-01

    Bronchial asthma is a worldwide disease with high incidence. It not only harms children's physical and mental health, but it also brings a heavy burden to their families as well as the society. However, the trigger and pathogenesis of the disease remain unclear. This study aimed to analyze TRPV1 gene mutation and expression of cytokines in children with acute bronchial asthma before and after treatment, thus providing theoretical guidance for the diagnosis and treatment of bronchial asthma in children. Real-time quantitative polymerase chain reaction was adopted to detect TRPV1 mRNA expression level and enzyme-linked immuno sorbent assay was used to detect the serum total IgE level, eosinophil (EOS) number, IL-4, IL-5, and interferon (IFN) gamma levels in peripheral venous blood of children in the healthy control group and asthma group before and after treatment. Logistic regression analysis was applied to analyze the most essential factor inducing bronchial asthma in children. TRPV1 mRNA level of peripheral blood in the asthma group was higher than that in the control group before treatment (p asthma in children. TRPV1 gene mutation was closely related to bronchial asthma in children, which provided a theoretical basis for the treatment and prognosis of children with bronchial asthma.

  17. Bronchial brush biopsies for studies of epithelial inflammation in stable asthma and nonobstructive chronic bronchitis.

    Science.gov (United States)

    Riise, G C; Andersson, B; Ahlstedt, S; Enander, I; Söderberg, M; Löwhagen, O; Larsson, S

    1996-08-01

    Recently, bronchial brush biopsy (BBB) has been introduced as a complimentary method to bronchial forceps biopsy for the study of bronchial epithelial cells. We wanted to determine whether epithelial inflammatory cells in bronchial brush biopsies can reflect mucosal inflammation assessed indirectly by levels of cellular activation markers in bronchial lavage fluid. We studied 15 healthy controls, 11 asthmatics with regular steroid inhalation therapy, 13 asthmatics without steroids, and 10 smokers with nonobstructive chronic bronchitis. Differential counts of epithelial and inflammatory cells were made from the BBB material. Bronchial lavage levels of eosinophil cationic protein (ECP), myeloperoxidase (MPO), tryptase, hyaluronan and interleukin-8 (IL-8) were measured as indirect markers for inflammatory cell activation. We found an increased percentage of eosinophil granulocytes in the BBB from the steroid-untreated asthmatic patients (1.16%) in comparison to the other groups (0.11%, 0.09% and 0.02%, respectively; pairways disease. These changes appear to relate to the degree of inflammatory activity and disease severity in asthma. PMID:8866592

  18. Bronchial asthma in elderlies: an approach to this issue in Cuba

    Directory of Open Access Journals (Sweden)

    Marisela Pérez Pacaréu

    2011-03-01

    Full Text Available Clinical and epidemiological behaviour of bronchial asthma in elderlies is almost an unknown topic in the investigations carried out in the field, because studies conducted in adult population approach a single age group from 15 years old on. Bronchial asthma is considered a health problem given its high prevalence: about 10% of the Cuban population suffers from it. In elderlies it is an underdiagnosed and undertreated disease often confused with chronic lung disease, so it is very important to able to evaluate the specific characteristics it presents in elderlies in order to attack the disease effectively, and promote the life quality of asthmatic elderlies.

  19. Stimulation of mucin secretion from human bronchial epithelial cells by mast cell chymase

    Institute of Scientific and Technical Information of China (English)

    Shao-heng HE; Jian ZHENG

    2004-01-01

    AIM: To investigate the effect ofchymase on the mucin secretion from human bronchial epithelial cells. METHODS:Primarily-cultured human bronchial epithelial (PCHBE) cells and normal human bronchial epithelial (NHBE) cells were cultured with chymase or other stimulus in a mixture of bronchial epithelial growth medium (BEGM) and Dulbecco's modified Eagle's medium (DMEM), and the quantities of stimulatory mucin release were recorded.MUC5AC mucin was measured with an ELISA and dolichos biflorus agglutinin (DBA) mucin was determined with an enzyme linked DBA assay. RESULTS: A dose-dependent secretion of DBA mucin from PCHBE cells was observed with chymase with a maximum secretion of 98 % above baseline being achieved following 3 h incubation.The action of chymase started from 1 h, peaked at 3 h and dramatically decreased at 20 h following incubation.Chymase was able to also stimulate approximately 38 % increase in MUC5AC mucin release from PCHBE cells, and about 121% increase in DBA mucin release from NHBE cells. A chymase inhibitor soybean trypsin inhibitor (SBTI)was able to inhibit up to 85 % chymase induced mucin release, indicating that the enzymatic activity was essential for the actions of chymase on bronchial epithelial cells. CONCLUSION: Chymase is a potent stimulus of mucin secretion from human bronchial epithelial cells. It can contribute to mucus hypersecretion process in the patients with chronic obstructive pulmonary disease or asthma.

  20. Environmental risk factors and allergic bronchial asthma.

    Science.gov (United States)

    D'Amato, G; Liccardi, G; D'Amato, M; Holgate, S

    2005-09-01

    The prevalence of allergic respiratory diseases such as bronchial asthma has increased in recent years, especially in industrialized countries. A change in the genetic predisposition is an unlikely cause of the increase in allergic diseases because genetic changes in a population require several generations. Consequently, this increase may be explained by changes in environmental factors, including indoor and outdoor air pollution. Over the past two decades, there has been increasing interest in studies of air pollution and its effects on human health. Although the role played by outdoor pollutants in allergic sensitization of the airways has yet to be clarified, a body of evidence suggests that urbanization, with its high levels of vehicle emissions, and a westernized lifestyle are linked to the rising frequency of respiratory allergic diseases observed in most industrialized countries, and there is considerable evidence that asthmatic persons are at increased risk of developing asthma exacerbations with exposure to ozone, nitrogen dioxide, sulphur dioxide and inhalable particulate matter. However, it is not easy to evaluate the impact of air pollution on the timing of asthma exacerbations and on the prevalence of asthma in general. As concentrations of airborne allergens and air pollutants are frequently increased contemporaneously, an enhanced IgE-mediated response to aeroallergens and enhanced airway inflammation could account for the increasing frequency of allergic respiratory allergy and bronchial asthma. Pollinosis is frequently used to study the interrelationship between air pollution and respiratory allergy. Climatic factors (temperature, wind speed, humidity, thunderstorms, etc) can affect both components (biological and chemical) of this interaction. By attaching to the surface of pollen grains and of plant-derived particles of paucimicronic size, pollutants could modify not only the morphology of these antigen-carrying agents but also their allergenic

  1. Classification, staging and radiotherapy of bronchial carcinoma

    International Nuclear Information System (INIS)

    This thesis reports a study performed to evaluate the stage classification of bronchial carcinoma published by Thomas in 1963. The study was done in the radiotherapy department of a teaching hospital, and had three parts: a comparative analysis of the classifications and stage divisions described in the literature on bronchial carcinoma; an evaluation of the theoretical basis of the classification system introduced by Thomas as well as of the practical applicability of the division into stages, with respect to the assessment of the prognosis and the choice of therapy; and an analysis of various aspects of irradiation as well as of a number of prognostic factors in bronchial carcinoma. (Auth.)

  2. Distribution of immunocompetent cells in the bronchial wall of clinically healthy subjects showing bronchial hyperresponsiveness.

    OpenAIRE

    Power, C; Sreenan, S; Hurson, B.; Burke, C; Poulter, L W

    1993-01-01

    BACKGROUND--Nearly all asthmatic subjects show bronchial hyperresponsiveness, in that the provocative concentration of histamine reducing forced expiratory volume in one second (FEV1) by 20% (PC20FEV1) is < or = 8 mg/ml histamine, and have underlying chronic inflammation of the bronchial wall mediated by T cells. The possible cause and effect relationship between these phenomena remains an enigma. As a proportion of clinically healthy subjects show bronchial hyperresponsiveness, this study wa...

  3. Bronchial asthma: correlation of high resolution computerized tomography findings with clinical data

    International Nuclear Information System (INIS)

    In this work we did a sectional study of 31 asthmatic patients with several levels of disease severity, which were submitted to high resolution computed tomography of the thorax and spirometry, between the months of July, 1995 and August, 1997. The tomographic findings were correlated with the clinical classification of the patients and the most frequent tomographic findings were bronchial wall thickening, bronchial dilatation, air trapping, centrilobular opacities, cicatricial linear shadows, mucoid impaction, emphysema and atelectasis. In asthmatic patients of long duration we observed small airway disease and irreversible lesions as the predominant findings. In smoking patients there was no high frequency of emphysema. (author)

  4. Upper respiratory tract disease and bronchial asthma

    Institute of Scientific and Technical Information of China (English)

    2005-01-01

    2005201 Role of dynamic changes of blood eosinophilia, eosinophil cationic protein and IgE in children with asthma. LOU Jin-tu (楼金吐)?,et al. Children’s Hosp.Med Coll .Zhejiang Univ.Hangzhou 310003. Chin J Emerg Med,2005;14(1) :68-70. Objective: To explore the roles and clinical significance of eosinophilia (EOS) ,eosinophil cationic protein (ECP),IgE and dust mite-skin test in children with asthma. Methods: Peripheral blood amount of EOS,serum concentration of ECP and IgE were de-

  5. Upper respiratory tract disease and bronchial asthma

    Institute of Scientific and Technical Information of China (English)

    1997-01-01

    970320 The role of Interleukin-4 and interferon-y inthe pathogenesis of asthma. WANG Huaqiang(王华强), et al. General Hosp, Jinan Command, Jinan250031. Med J Chin PLA 1997; 22(1): 57-59 Objective: To study the role of interleukin-4(IL-4)and interferon-γ(IFN-7)in the pathogenesis of asth-

  6. Upper respiratory tract disease and bronchial asthma

    Institute of Scientific and Technical Information of China (English)

    1993-01-01

    930119 Lung sound analysis of upper airwayobstruction.SHI Yi(施毅),et al.Dept RespirMed,General Hosp,Nanjing Command,210002.Chin J Tuberc & Respir Dis 1992;15(4):228-230.The frequency spectrum of stridor was stud-ied by the computer program in the patientswith upper airway obstruction.The resultsshowed that the peak frequency of respiratorysound increased significantly,the frequencyspectrum got wider and moved to the high fre-quency area above 200Hz and the E ratio wassmaller than 1.These changes were more ap-

  7. Permanent Cortical Blindness After Bronchial Artery Embolization

    Energy Technology Data Exchange (ETDEWEB)

    Doorn, Colette S. van, E-mail: cvandoorn@gmail.com; De Boo, Diederick W., E-mail: d.w.deboo@amc.uva.nl [Academic Medical Centre, Department of Radiology (Netherlands); Weersink, Els J. M., E-mail: e.j.m.weersink@amc.uva.nl [Academic Medical Centre, Department of Pulmonology (Netherlands); Delden, Otto M. van, E-mail: o.m.vandelden@amc.uva.nl; Reekers, Jim A., E-mail: j.a.reekers@amc.uva.nl; Lienden, Krijn P. van, E-mail: k.p.vanlienden@amc.uva.nl [Academic Medical Centre, Department of Radiology (Netherlands)

    2013-12-15

    A 35-year-old female with a known medical history of cystic fibrosis was admitted to our institution for massive hemoptysis. CTA depicted a hypertrophied bronchial artery to the right upper lobe and showed signs of recent bleeding at that location. Bronchial artery embolization (BAE) was performed with gelfoam slurry, because pronounced shunting to the pulmonary artery was present. Immediately after BAE, the patient developed bilateral cortical blindness. Control angiography showed an initially not opacified anastomosis between the embolized bronchial artery and the right subclavian artery, near to the origin of the right vertebral artery. Cessation of outflow in the bronchial circulation reversed the flow through the anastomosis and allowed for spill of embolization material into the posterior circulation. Unfortunately the cortical blindness presented was permanent.

  8. Selective bronchial intubation for pulmonary emphysema.

    OpenAIRE

    Campbell, A N; Zarfin, Y; Perlman, M

    1984-01-01

    Two neonates with respiratory distress syndrome developed unilateral pulmonary interstitial emphysema causing mediastinal shift and compressive atelectasis. Treatment with contralateral bronchial intubation for five days proved to be life saving.

  9. Permanent Cortical Blindness After Bronchial Artery Embolization

    International Nuclear Information System (INIS)

    A 35-year-old female with a known medical history of cystic fibrosis was admitted to our institution for massive hemoptysis. CTA depicted a hypertrophied bronchial artery to the right upper lobe and showed signs of recent bleeding at that location. Bronchial artery embolization (BAE) was performed with gelfoam slurry, because pronounced shunting to the pulmonary artery was present. Immediately after BAE, the patient developed bilateral cortical blindness. Control angiography showed an initially not opacified anastomosis between the embolized bronchial artery and the right subclavian artery, near to the origin of the right vertebral artery. Cessation of outflow in the bronchial circulation reversed the flow through the anastomosis and allowed for spill of embolization material into the posterior circulation. Unfortunately the cortical blindness presented was permanent

  10. Dynamic Properties of Human Bronchial Airway Tissues

    OpenAIRE

    Wang, Jau-Yi; Mesquida, Patrick; Pallai, Prathap; Corrigan, Chris J; Lee, Tak H

    2011-01-01

    Young's Modulus and dynamic force moduli were measured on human bronchial airway tissues by compression. A simple and low-cost system for measuring the tensile-strengh of soft bio-materials has been built for this study. The force-distance measurements were undertaken on the dissected bronchial airway walls, cartilages and mucosa from the surgery-removed lungs donated by lung cancer patients with COPD. Young's modulus is estimated from the initial slope of unloading force-displacement curve a...

  11. Reducing asthma attacks in patients with severe asthma: The role of bronchial thermoplasty.

    Science.gov (United States)

    Dunn, Ryan; Wechsler, Michael E

    2015-01-01

    Asthma remains one of the most common diseases worldwide and results in significant societal health care costs and in morbidity and mortality to those afflicted. Despite currently available medications, 5-10% of patients with asthma have severe disease with debilitating symptoms and/or life-threatening exacerbations. Bronchial thermoplasty is a device-based therapy with proven efficacy in this subgroup of patients. Thus far, bronchial thermoplasty has been shown to reduce exacerbations and to improve important measures of asthma control. The purpose of this article is to review the pathophysiology of severe asthma, including the role of airway smooth muscle cells and the procedural aspects of bronchial thermoplasty, and to review the evidence behind this important therapy. PMID:26108080

  12. VATS right upper lobe bronchial sleeve resection

    Science.gov (United States)

    Ma, Qianli

    2016-01-01

    Background The aim of this study is to discuss video-assisted thoracic surgery (VATS) sleeve bronchial lobectomy when handling the locally advanced central lung cancer (involving the trachea and/or main bronchus). Methods A 2.5 cm × 1.0 cm mass was found in the right upper lobe. Bronchoscopy demonstrated the tumor obstructing the right upper lobe bronchus and involved the right main bronchus and bronchus intermedius. Interrupted sutures were chosen for bronchial anastomosis. Bronchial membrane was sutured first, and then circumference end-to-end anastomoses were carried out using 3-0 absorbable sutures. Results There were no complications and the patient was discharged 8 days postoperatively. Conclusions The third intercostal space of the anterior axillary line was suggested for right upper lobe bronchial sleeve resection. This incision can reduce the distance and angle between the anastomosis to the incision, and facilitate anastomosis. This approach can also prevent operator from fatigue for keeping one posture for a long time. Clearance of the mediastinal lymph nodes before cutting the bronchus was helpful for exposing the right main bronchus, the upper lobe bronchus and bronchus intermedius satisfied. And this option would avoid pulling bronchial anastomosis during mediastinal lymph nodes clearance. Interrupted suture was safe and effective for VATS bronchial anastomosis. PMID:27621889

  13. Clinical and pathological features and imaging manifestations of bronchial anthracofibrosis: the findings in 15 patients

    Institute of Scientific and Technical Information of China (English)

    HAN Feng-feng; YANG Tian-yun; SONG Lin; ZHANG Yue; LI Hui-min; GUAN Wen-bin; LIU Qian

    2013-01-01

    Background Bronchial anthracofibrosis (BAF) has been defined as a luminal narrowing associated with anthracotic pigmentation on bronchoscopy without a relevant history of pneumoconiosis or smoking.The aim of the study is to study the clinical features and imaging manifestations of BAF,and to promote the awareness of this disease.Method Between October 2006 and January 2012,15 patients were diagnosed at our department as BAF that showed a narrowing or obliteration of lobar or segmental bronchi with anthracotic pigmentation in the overlying mucosa on bronchoscopy.The medical records including clinical features,imaging manifestations,electronic bronchoscopic findings,and pathological features were analysed,and the literature was reviewed.Results A total of 15 patients were analyzed; 13 were female (86.7%) and two were male (13.3%) and the age range was from 62 to 86 years with a mean age of 74.5 years.Three cases (20.0%) had a history of tuberculosis.The most common clinical symptoms of BAF were cough (100%),expectoration (73.3%),dyspnea (60.0%),and fever (46.7%).Twelve cases displayed mild to moderate obstructive ventilatory dysfunction.In the electronic bronchoscopic evaluation,the most common findings were black bronchial mucosal pigmentation,bronchial stenosis,bronchial occlusion,and bronchial mucosal folds.Pathological evaluation revealed chronic inflammation of the bronchial mucosa,submucosal carbon particle deposition,and mucosal or submucosal fibrosis.Chest CT scans showed that 15 patients had bronchial stenosis or obstruction (direct signs) with the right middle lobe being the most common site (11 cases,73.3%).The indirect sign was mainly the presence of bronchial obstructive diseases (including secondary infection),represented by 11 cases of pulmonary consolidation (73.3%),seven cases of atelectasis (46.7%),and five cases of nodules (33.3%).The CT mediastinal window showed bronchial lymph node lesions,mediastinal lymph node

  14. Airway vascular reactivity and vascularisation in human chronic airway disease

    NARCIS (Netherlands)

    Bailey, Simon R; Boustany, Sarah; Burgess, Janette K; Hirst, Stuart J; Sharma, Hari S; Simcock, David E; Suravaram, Padmini R; Weckmann, Markus

    2009-01-01

    Altered bronchial vascular reactivity and remodelling including angiogenesis are documented features of asthma and other chronic inflammatory airway diseases. Expansion of the bronchial vasculature under these conditions involves both functional (vasodilation, hyperperfusion, increased microvascular

  15. [Non-specific bronchial hyper-responsiveness and polymorphysm of xenobiotics biotransformation GSTM1 and GSTT1 genes under neutrophilic bronchial asthma in children].

    Science.gov (United States)

    Ivanova, L A; Mykaliuk, L V; Hryhola, O H

    2014-01-01

    With a view to study the effect of genes GSTT1 and GSTM1 deletion on the non-specific bronchial hyperresponsiveness in children with neutrophilic bronchial asthma (BA) 46 school age children having neutrophilic BA (1st clinical group) and their 48 coevals with eosinophilic phenotype of the disease (2nd clinical group) were subjected to a complex examination at the pulmo-allergologic department of the regional child clinical hospital of Chernivtsi. The study proved that genotype T1+M1del was more frequently registered in patients with the neutrophilic phenotype of the disease, and genotype T1delM1del was equifrequent in patients with different types of the inflammation of the respiratory ways. In patients with neutrophilic BA and deletion polymorphism of genes GSTT1 and GSTM1, there was a tendency to decreasing of the bronchial lability index through the decrease of bronchodilation, and bronchial response to histamine occurred to be higher than in children with the absence of polymorphism of the referred genes of the xenobiotics biotransformation system. PMID:24908960

  16. Endobronchial amyloidosis mimicking bronchial asthma: a case report and review of the literature

    Directory of Open Access Journals (Sweden)

    Kang Hyun-Wook

    2016-06-01

    Full Text Available Among two tracheobronchial forms (local and diffuse and two parenchymal forms (nodular and alveolar septal that were reported in previous literature, localized endobronchial amyloidosis is an uncommon disease of unknown cause. Bronchial amyloid deposits can occur as focal nodules or multifocal infiltration of the submucosa. We report the case of a 47-year-old man who had complained of dyspnea and wheezing for 1 month and who had been treated for severe asthma at another hospital. Endobronchial amyloidosis was confirmed by histological examination of the bronchial biopsies.

  17. Clinical significance of determination of serum TNF, IL-8 and GM-CSF levels in pediatric patients with bronchial asthma

    International Nuclear Information System (INIS)

    Objective: To investigate the clinical significance of changes of serum TNF, IL-8 and GM-CSF in pediatric patients with bronchial asthma. Methods: Serum TNF, IL-8 and GM-CSF levels were measured with RIA in 32 pediatric patients with bronchial asthma and 30 controls. Results: Serum levels of TNF, IL-8 and GM-CSF were very significantly higher in pediatric patients with bronchial asthma than those in controls (P<0.01). After one week treatment, the levels dropped considerably but still remained significantly higher than those in controls (P<0.05). Conclusion: These cytokines participated in the pathogenesis of bronchial asthma. Monitoring the changes of their serum levels was helpful for the management of the diseases. (authors)

  18. CHARACTERIZATION OF VEGETATIVE CHANGES IN BRONCHIAL ASTHMA OF VARIOUS SEVERITIES IN CHILDREN

    Directory of Open Access Journals (Sweden)

    Hurmatoy Khankeldieva

    2015-06-01

    Full Text Available This article presents a study of the state of vegetative balance and prognostic possibilities of quantitative parameters of functional activity of VNS to estimate a state of bodily adaptive reserves in children with severe BA. Bronchial asthma, being one of the most common chronic diseases in childhood, requires further research into predisposing and causally-significant factors, mechanisms of pathogenesis and peculiarities of a clinical course, improvement of methods of diagnostics, treatment, and prevention. Great importance in the development of bronchial asthma in children is given to genetically-fixed functional insufficiency of barrier tissues of the respiratory system, increased ability to synthesize allergic antibodies and produce certain cytokines, high bronchopulmonary sensitivity to mediators of allergic inflammation, lowered sensitivity of 3g-adrenoreceptors to endogenous catecholamins, changes in immunologic responsiveness, and disorder of neuroendocrinal regulation of immune responses. Implementation of genetic predisposition to bronchial asthma development is promoted by influence of adverse environmental factors.

  19. Eosinophilic Pneumonia in a Patient with Bronchial Myiasis: Case report and literature review.

    Science.gov (United States)

    Aich, Arindom; Al-Ismaili, Suad; Ramadhan, Fatma A; Al-Wardi, Talal H M; Al-Salmi, Quasem; Al-Hashami, Hilal

    2015-11-01

    Pulmonary myiasis is an unusual form of myiasis in humans and has been recently identified as a cause of eosinophilic pneumonia. We report the case of a 13-year-old Omani boy who presented to the Royal Hospital, Muscat, Oman, in October 2014 with respiratory distress. Bronchial aspirates revealed features of eosinophilic pneumonia. Possible larvae identified in the cytology report, a high immunoglobulin E level and the patient history all indicated bronchial myiasis. The patient was treated with steroids and ventilation and has since been disease-free with no long-term side-effects. To the best of the authors' knowledge, this is the first case of bronchial myiasis in Oman. PMID:26629385

  20. Determination of blood ET-1 and related cytokines in pediatric patients with bronchial asthma

    International Nuclear Information System (INIS)

    Objective: To explore the role of endothelin and related cytokines (IL-5, IL-6, IL-8) in the development of bronchial asthma in pediatric patients. Methods: Plasma ET-1 (with RIA) and serum IL-5 (with ELISA), IL-6, IL-8 (with RIA) levels were measured in 42 pediatric patients with bronchial asthma and 30 controls. Results: The plasma ET-1 levels in the patients during acute attack (n=22) were significantly higher than those in patients during remission (n=20) as well as in controls (P0.05). Levels of ET-1 were positively correlated with those of IL-5 (during attack, r=0.560, P<0.01; during remission, r=0.435, P<0.01). Conclusion: ET and the cytokines IL-5, IL-6, IL-8 participated in the pathogenesis of bronchial asthma through different mechanisms and dynamic determination of which could reflect the progression of the disease. (authors)

  1. The response of human nasal and bronchial organotypic tissue cultures to repeated whole cigarette smoke exposure.

    Science.gov (United States)

    Talikka, Marja; Kostadinova, Radina; Xiang, Yang; Mathis, Carole; Sewer, Alain; Majeed, Shoaib; Kuehn, Diana; Frentzel, Stefan; Merg, Celine; Geertz, Marcel; Martin, Florian; Ivanov, Nikolai V; Peitsch, Manuel C; Hoeng, Julia

    2014-01-01

    Exposure to cigarette smoke (CS) is linked to the development of respiratory diseases, and there is a need to understand the mechanisms whereby CS causes damage. Although animal models have provided valuable insights into smoking-related respiratory tract damage, modern toxicity testing calls for reliable in vitro models as alternatives for animal experimentation. We report on a repeated whole mainstream CS exposure of nasal and bronchial organotypic tissue cultures that mimic the morphological, physiological, and molecular attributes of the human respiratory tract. Despite the similar cellular staining and cytokine secretion in both tissue types, the transcriptomic analyses in the context of biological network models identified similar and diverse biological processes that were impacted by CS-exposed nasal and bronchial cultures. Our results demonstrate that nasal and bronchial tissue cultures are appropriate in vitro models for the assessment of CS-induced adverse effects in the respiratory system and promising alternative to animal experimentation. PMID:25297719

  2. Relation between duration of smoking cessation and bronchial inflammation in COPD

    NARCIS (Netherlands)

    Lapperre, TS; Postma, DS; Gosman, M.M.E.; Snoeck-Stroband, JB; ten Hacken, NHT; Hiemstra, PS; Timens, W; Sterk, PJ; Mauad, T

    2006-01-01

    Background: Chronic obstructive pulmonary disease (COPD) is associated with airway inflammation. Although smoking cessation improves symptoms and the decline in lung function in COPD, it is unknown whether bronchial inflammation in patients with established COPD varies with the duration of smoking c

  3. Pulmonary interstitial emphysema: selective bronchial occlusion with a Swan-Ganz catheter.

    OpenAIRE

    Lewis, S; Pelausa, E; Ojah, C; Paes, B.

    1988-01-01

    A 26 week preterm infant ventilated for hyaline membrane disease developed severe pulmonary interstitial emphysema with extensive right sided bullous formation, mediastinal shift, and subsequent left sided atelectasis. A paediatric Swan-Ganz catheter was used for selective bronchial occlusion with dramatic improvement in the infant's clinical condition and radiographic findings.

  4. [Cytomorphological analysis of remodeling of the bronchial wall in different types of bronchial asthma].

    Science.gov (United States)

    Gereng, E A; Sukhodolo, I V; Pleshko, R I; Ogorodova, L M; Selivanova, P A; Dziuman, A N

    2012-01-01

    The objective of the present work was to search for the tissue and cellular markers of remodeling of bronchial mucosa in the patients with different clinical forms of bronchial asthma (BA). The use of up-to-date morphometric techniques has demonstrated that mild and moderately severe forms of bronchial asthma are accompanied by the development of Th2-immune response associated with increased production of interleukin-4 and marked degranulation of eosinophilic granulocytes resulting in desquamation of epithelium and goblet cell hyperplasia. The severe BA phenotype of "chronic asthma with fixed obstruction" is associated with the development of non-atopic inflammation in the bronchial mucous membrane that manifests itself as the increased concentration of interleukin-8 in bronchial mucosa and its neutrophilic infiltration leading to the development of pronounced subepithelial fibrosis, thickening of the basal membrane, and atrophy of epithelium. Specific structural changes in bronchial mucosa of the patients presenting with BA underlie functional disturbances that cause severe bronchial obstructive syndrome. PMID:22645957

  5. Chinese and Uighur medicine diagnostic criteria of the evaluation of the Modern drug treatment side-effects in bronchial asthma patients

    Institute of Scientific and Technical Information of China (English)

    Dubrovin Denis; Igor Gogol; Mikhail Baranov; Nurmuhammat Amat; Halmurat Upur

    2013-01-01

    We studied Chinese and Uighur medicines and create an automated computer diagnostics system according to principals of Uighur medicine for evaluation of bronchial asthma patient′s state. 498 patients with bronchial asthma were enrolled the automated computer diagnostic program. 304 patients were evaluated in the process of drug and non-drug treatment. Savda asthma type of Uighur medicine do not corresponds with any of the defined clinico-pathogenetic variants of the disease. Thus, prevailing of atopic bronchial asthma with probability of 0.7 is defined with Savda categories with deficiency' (P<0.01) variants in Chinese Medicine. The combination of infectious-dependent variant of bronchial asthma Abnormal Savda syndrome with Abnormal Savda syndrome bronchial asthma in 71% of cases have prevailing harder case of Abnormal Savda in 74% of cases belongs to the categories of an aspirin component in the pathogenesis of mixed asthma, in 83% of cases

  6. Chronic Pulmonary Aspergillosis Complicating Bronchial Atresia

    Directory of Open Access Journals (Sweden)

    Mazen O. Al-Qadi

    2014-01-01

    Full Text Available Bronchial atresia is a rare pulmonary developmental anomaly characterized by the presence of a focal obliteration of a segmental or lobar bronchial lumen. The lung distal to the atretic bronchus is typically emphysematous along with the presence of mucus filled ectatic bronchi (mucoceles. BA is usually asymptomatic but pulmonary infections can rarely develop in the emphysematous lung distal to the atretic bronchus. We present a unique case of chronic pulmonary aspergillosis (CPA in a patient with BA with no evidence of immune dysfunction. The patient was treated initially with voriconazole and subsequently underwent surgical excision of the involved area. On follow-up, she has done extremely well with no evidence for recurrence. In summary, we describe the first case of chronic pulmonary aspergillosis in an immunocompetent patient with bronchial atresia.

  7. Bronchial diverticula in smokers on thin-section CT

    Energy Technology Data Exchange (ETDEWEB)

    Sverzellati, Nicola; Ingegnoli, Anna [University of Parma, Department of Clinical Sciences, Division of Radiology, Parma (Italy); Calabro, Elisa; Pastorino, Ugo [National Cancer Institute, Division of Thoracic Surgery, Milan (Italy); Randi, Giorgia; La Vecchia, Carlo [Mario Negri Institute, Department of Epidemiology, Milan (Italy); University of Milano, Institute of Medical Statistics and Biometry ' ' G. A. Maccacaro' ' , Milan (Italy); Marchiano, Alfonso [National Cancer Institute, Division of Radiology, Milan (Italy); Kuhnigk, Jan-Martin [Fraunhofer MEVIS - Institute for Medical Image Computing, Bremen (Germany); Hansell, David M. [Royal Brompton Hospital, Department of Radiology, London (United Kingdom); Zompatori, Maurizio [S. Orsola-Malpighi Hospital, Department of Radiology, Bologna (Italy)

    2010-01-15

    The objective was to determine the prevalence of bronchial diverticula in smokers on thin-section CT and the relationship to clinical and other morphological features on CT. Thin-section CT images of 503 cigarette smokers were assessed for the profusion and location of diverticula in the major airways. The extent of the bronchial diverticula was recorded as follows: grade 0, none; grade 1, one to three diverticula; grade 2, more than three diverticula. The extent of emphysema, bronchial wall thickness, clinical features, and pulmonary function were compared in the sub-groups stratified according to the extent of bronchial diverticula. A total of 229/503 (45.5%) smokers had bronchial diverticula, with 168/503 (33.3%) and 61/503 (12.2%) having grade 1 and 2 bronchial diverticula respectively. Subjects with grade 2 bronchial diverticula were heavier smokers, reported a history of coughing more frequently, and showed more severe functional impairment, greater extent of emphysema and more severe bronchial wall thickening compared with subjects with grade 1 and those individuals without bronchial diverticula (P<0.05). Multivariate regression analysis revealed that only bronchial wall thickness predicted the extent of the bronchial diverticula (P<0.0001). Bronchial diverticula are a frequent finding in the major airways of smokers, and they are associated with other markers of smoking-related damage. (orig.)

  8. Radiologic management of haemoptysis. Diagnostic and interventional bronchial arterial embolisation

    Energy Technology Data Exchange (ETDEWEB)

    Ittrich, H.; Adam, G. [Univ. Medical Center Hamburg, Hamburg (Germany). Diagnostic and Interventional Radiology Dept. and Clinic; Klose, H. [Univ. Medical Center Hamburg, Hamburg (Germany). Section Pneumology

    2015-04-15

    Hemoptysis can be a life-threatening pulmonary emergency with high mortality, is symptomatic of an underlying severe pulmonary disease and requires immediate diagnosis and treatment. Diagnostically, bronchoscopy, conventional chest x-ray and contrast-enhanced multislice computed tomography (MSCT) with CT angiography (CTA) provide information regarding the underlying pulmonary disease, bleeding site, the vascular anatomy of the bronchial arteries (BA) and extrabronchial branches, as well a basis for planning of endovascular intervention. Therapeutically, bronchial artery embolization (BAE) is a safe and effective technique in the hands of an experienced interventionist with profound knowledge of the BA anatomy and possible pitfalls as well as experience with first-line therapy of recurrent and massive hemoptysis or as an intervention prior to elective surgery. Recurrent episodes of hemoptysis are not uncommon and require a prompt repeat BAE after exclusion of extrabronchial systemic and pulmonary artery bleeding sources. This review article should give an overview of the history, anatomical and pathophysiological basics and the clinical context of hemoptysis and diagnosis, as well as a survey of management, treatment and results of BAE.

  9. Epidemiological & Risk Factors In Childhood Bronchial Asthma

    Directory of Open Access Journals (Sweden)

    Singh Harmesh

    1998-01-01

    Full Text Available Research question: What are the epidemiological and risk factors associated with asthma in children. Objective: To determine epidemiological and risk factors in childhood bronchial asthma. Study design: Cross-sectional. Setting Hospital based. Participants: Children suffering from bronchial asthma and their parents/ attendants. Sample size: 235 children. Study variables: Age, sex place of residence, socio-economic status, age of onset of asthma, no of siblings, fuel used for cooking, smoking, asthma in the family seasonal variation etc. Statistical analysis: percentages

  10. Bronchial carcinoid tumors: clinical and radiological findings in 21 patients

    International Nuclear Information System (INIS)

    Bronchial carcinoid tumors are neoplasms that range from typical carcinoid s to the more aggressive atypical ones with similar imaging features. Considering the low prevalence of the tumor, we reviewed 21 patients. Patients and methods: in this retrospective observational study, we reviewed the clinical, pathological and imaging findings in 21 patients diagnosed with bronchial carcinoid tumors who were admitted to National Research Institute of Tuberculosis and Lung Disease in a 6 years period. Plain radiography and CT scan of patients were reviewed. Results: 52.4% of the patients were male and 47.6 % female. The mean age of patients was 39.1 years. The most frequent clinical symptoms were cough (n=15; 71.4%), hemoptysis (n.13; 61.9%). dyspnea (n=12; 57.1%). and pleuritic chest pain (n.7; 19%). Most of the lesions (68%) were in the right lung, 24% in the left lung. 8% in the trachea, and 19% were bilateral. Right upper lobe and right intermediate bronchus (24% and 32% respectively) were the most frequent sites of tumor. In radiologic examination, two cases (9.5%) had normal CXRs. Pulmonary collapse (52.4%) and mass (23.8%) were the most frequent findings. Reviewing CT scans showed endobronchial lesion in three (15%) and mass in 11 (55%) patients. The mass was well defined in 8 cases (72%) and ill-defined in 3 (28%). Other findings were: mediastinal invasion (45%), atelectasis (55%). post obstructive pneumonitis (2 0%) and eccentric calcification (15%). Pathological examination revealed typical (n=18: 86% ) and atypical (n.3: 14%) carcinoid tumors. Conclusion: although rare, bronchial carcinoid tumors should be considered a differential diagnosis in adult patients with cough, hemoptysis, dyspnea, and pleuritic chest pain. Chest xray and CT scan can help with diagnosis. Major imaging findings are central, round or ovoid well-defined mass, and obstruction signs like at electasis. Diagnosis and classification should be confirmed by pathological examination on sample

  11. 支气管哮喘患儿不同治疗方法内皮素和降钙素基因相关肽结果比较%Study of endothelin and calcitonin gene-related peptide levels in children with bronchial asthma by different therapies

    Institute of Scientific and Technical Information of China (English)

    吕汉文; 卢远达; 徐淑兰; 叶彩丽; 林灿华; 周翔

    2002-01-01

    Background: Bronchial asthma is an allergic pulmonary disease. The attack is mostly induced by the inhalation of or contact with allergens, such as pollens, dusts, insects (such as mite), germs, etc. During the attack, patients have severe dyspnea which is due to spasm of bronchial smooth muscles, swelling of the bronchial mucosa, and hypersecretion of the mucus, leading to bronchial obstruction. To observe the endothelin(ET) and calcitonin gene related peptide(CGRP) changes in treating children with bronchial asthma by dopamine( DP), dobutamine( DOB),and regitine.

  12. Cigarette Smoke and the Induction of Urokinase Plasminogen Activator Receptor In Vivo: Selective Contribution of Isoforms to Bronchial Epithelial Phenotype.

    Science.gov (United States)

    Portelli, Michael A; Stewart, Ceri E; Hall, Ian P; Brightling, Christopher E; Sayers, Ian

    2015-08-01

    The urokinase plasminogen activator receptor (uPAR) gene (PLAUR) has been identified as an asthma susceptibility gene, with polymorphisms within that gene being associated with baseline lung function, lung function decline, and lung function in a smoking population. Soluble cleaved uPAR (scuPAR), a molecule identified as a marker of increased morbidity and mortality in a number of diseases, has been shown to be elevated in the airways of patients with asthma and in patients with chronic obstructive pulmonary disease. However, the functionality of soluble receptor isoforms and their relationship with an important initiator for obstructive lung disease, cigarette smoke, remains undefined. In this study, we set out to determine the effect of cigarette smoke on soluble uPAR isoforms, its regulatory pathway and the resultant effect on bronchial epithelial cell function. We identified a positive association between cigarette pack-years and uPAR expression in the airway bronchial epithelium of biopsies from patients with asthma (n = 27; P = 0.0485). In vitro, cigarette smoke promoted cleavage of uPAR from the surface of bronchial epithelial cells (1.5× induction; P bronchial epithelial cells. This suggests that cigarette smoke elevates soluble receptor isoforms in bronchial epithelial cells through direct (cleavage) and indirect (messenger RNA expression) means. These findings provide further insight into how cigarette smoke may influence changes in the airways of importance to airway remodeling and obstructive lung disease progression. PMID:25490122

  13. Infection by Ascaris lumbricoides and bronchial hyper reactivity: an outstanding association in Venezuelan school children from endemic areas.

    Science.gov (United States)

    Hagel, I; Cabrera, M; Hurtado, M A; Sanchez, P; Puccio, F; Di Prisco, M C; Palenque, M

    2007-09-01

    Asthma and other respiratory diseases have increased in the last years among Venezuelan children from helminthic endemic areas where the infection by Ascaris lumbricoides has been associated to bronchial airway inflammation in parasitized individuals. The aim of this work was to investigate the possible associations between the development of bronchial hyper reactivity and the immune response against A. lumbricoides in urban and rural children. We evaluated 470 school children from rural and urban communities. Pulmonary function tests were performed and >or=20% PC(20) changes were considered as a positive diagnostic of bronchial hyper reactivity. The prevalence and intensity of A. lumbricoides infection was determined by faecal examination. Specific serum IgE levels using a modified ELISA and skin prick tests against A. lumbricoides and the common allergen Dermatophagoides pteronyssinus were done. The number of circulating lymphocyte sub populations was determined by flow cytometry analysis. In rural children, bronchial hyper reactivity was associated with increased specific levels of anti-A. lumbricoides IgE (plumbricoides (plumbricoides IgE levels. Elevated numbers of circulating CD3+CD4+ and CD20+CD23+ cells were found in rural children with bronchial hyper reactivity compared to their asymptomatic counterparts. They correlated positively with anti-A. lumbricoides IgE levels (plumbricoides infection may be involved in the development of bronchial hyper reactivity among rural children from endemic areas and also that improved hygienic conditions in the urban environment is associated with increased responses to airborne allergens.

  14. Relation between the bronchial obstructive response to inhaled lipopolysaccharide and bronchial responsiveness to histamine.

    OpenAIRE

    Michel, O; Ginanni, R; Sergysels, R

    1992-01-01

    BACKGROUND: Bronchoconstriction has developed after inhalation of lipopolysaccharide in a dose of 20 micrograms in asthmatic patients and of 200 micrograms in normal subjects. This study set out to determine whether the bronchial response to lipopolysaccharide was related to non-specific bronchial responsiveness and atopy. METHODS: Sixteen subjects with a fall in specific airway conductance of 40% (PD40sGaw) after inhaling up to 900 micrograms histamine inhaled 20 micrograms lipopolysaccharid...

  15. DNA repair in human bronchial epithelial cells

    International Nuclear Information System (INIS)

    The purpose of this investigation was to compare the response of human cell types (bronchial epithelial cells and fibroblasts and skin fibroblasts) to various DNA damaging agents. Repair of DNA single strand breaks (SSB) induced by 5 krads of X-ray was similar for all cell types; approximately 90% of the DNA SSB were rejoined within one hour. During excision repair of DNA damage from u.v.-radiation, the frequencies of DNA SSB as estimated by the alkaline elution technique, were similar in all cell types. Repair replication as measured by BND cellulose chromatography was also similar in epithelial and fibroblastic cells after u.v.-irradiation. Similar levels of SSB were also observed in epithelial and fibroblastic cells after exposure to chemical carcinogens: 7,12-dimethylbenz[a]anthracene; benzo[a]pyrene diol epoxide (BPDE); or N-methyl-N-nitro-N-nitrosoguanidine. Significant repair replication of BPDE-induced DNA damage was detected in both bronchial epithelial and fibroblastic cells, although the level in fibroblasts was approximately 40% of that in epithelial cells. The pulmonary carcinogen asbestos did not damage DNA. DNA-protein crosslinks induced by formaldehyde were rapidly removed in bronchial cells. Further, epithelial and fibroblastic cells, which were incubated with formaldehyde and the polymerase inhibitor combination of cytosine arabinoside and hydroxyurea, accumulated DNA SSB at approximately equal frequencies. These results should provide a useful background for further investigations of the response of human bronchial cells to various DNA damaging agents

  16. Clavicular osteoma associated with bronchial osteomas

    Energy Technology Data Exchange (ETDEWEB)

    Saglik, Yener; Yiliz, H. Yusuf; Erakar, Aziz [Department of Orthopaedic Surgery, Ankara University School of Medicine, 06100, Ankara (Turkey); Kendi, Tuba Karaguelle [Integra MR Imaging Center, Ankara (Turkey); CMRR, University of Minnesota, 2021 6th Street SE, MN 55455, Minneapolis (United States); Guengoer, Adem [Department of Chest Surgery, Ankara University School of Medicine, 06100, Ankara (Turkey); Erekul, Selim [Department of Pathology, Ankara University School of Medicine, 06100, Ankara (Turkey)

    2004-04-01

    Osteoma is a rare benign tumor, composed of bony tissues. It predominantly involves the skull but rarely the long bones. In this report we present a case of clavicular osteoma associated with bronchial osteomas. This association has not previously been reported. There was no evidence of Gardner's syndrome. (orig.)

  17. Anti-apoptotic effects of Z alpha1-antitrypsin in human bronchial epithelial cells.

    LENUS (Irish Health Repository)

    Greene, C M

    2010-05-01

    alpha(1)-antitrypsin (alpha(1)-AT) deficiency is a genetic disease which manifests as early-onset emphysema or liver disease. Although the majority of alpha(1)-AT is produced by the liver, it is also produced by bronchial epithelial cells, amongst others, in the lung. Herein, we investigate the effects of mutant Z alpha(1)-AT (ZAAT) expression on apoptosis in a human bronchial epithelial cell line (16HBE14o-) and delineate the mechanisms involved. Control, M variant alpha(1)-AT (MAAT)- or ZAAT-expressing cells were assessed for apoptosis, caspase-3 activity, cell viability, phosphorylation of Bad, nuclear factor (NF)-kappaB activation and induced expression of a selection of pro- and anti-apoptotic genes. Expression of ZAAT in 16HBE14o- cells, like MAAT, inhibited basal and agonist-induced apoptosis. ZAAT expression also inhibited caspase-3 activity by 57% compared with control cells (p = 0.05) and was a more potent inhibitor than MAAT. Whilst ZAAT had no effect on the activity of Bad, its expression activated NF-kappaB-dependent gene expression above control or MAAT-expressing cells. In 16HBE14o- cells but not HEK293 cells, ZAAT upregulated expression of cIAP-1, an upstream regulator of NF-kappaB. cIAP1 expression was increased in ZAAT versus MAAT bronchial biopsies. The data suggest a novel mechanism by which ZAAT may promote human bronchial epithelial cell survival.

  18. Assessment of quality of life in bronchial asthma patients

    Directory of Open Access Journals (Sweden)

    N Nalina

    2015-01-01

    Full Text Available Introduction: Asthma is a common chronic disease that affects persons of all ages. People with asthma report impact on the physical, psychological and social domains of quality of life. Health-related quality of life (HRQoL measures have been developed to complement traditional health measures such as prevalence, mortality and hospitalization as indicators of the impact of disease. Objective and Study Design: The objective of this study was to assess HRQoL in Bronchial asthma patients and to relate the severity of asthma with their quality of life. About 85 asthma patients were evaluated for HRQoL and their pulmonary function tests values were correlated with HRQoL scores. Results and Conclusion: It was found that asthma patients had poor quality of life. There was greater impairment in quality of life in females, obese and middle age patients indicating that sex, body mass index and age are determinants of HRQoL in asthma patients.

  19. Lung function and bronchial reactivity in farmers.

    Science.gov (United States)

    Iversen, M; Dahl, R; Jensen, E J; Korsgaard, J; Hallas, T

    1989-01-01

    The purpose of this study was to evaluate the prevalence and type of lung function disorders in Danish farmers. Three samples of farmers were drawn from a group of unselected farmers who had participated in an epidemiological study. Group I (47 persons) was a sample of the 8% of all farmers who had reported that they had asthma; group II (63 persons) was a sample of the 28% of farmers who had had wheezing, shortness of breath, or cough without phlegm; and group III (34 persons) a sample of the farmers (64% of the total) who had no asthma and no respiratory symptoms. The farmers with symptoms (groups I and II) had low mean levels of FEV1 and high values for residual volume, whereas the symptomless farmers had normal lung function and no airways obstruction. The proportion of farmers with an FEV1 below the 95% confidence limit for predicted values was 43% in group I and 23% in group II; there were none in group III. Bronchial hyperreactivity to histamine occurred in 96% of asthmatic farmers, 67% of farmers with wheezing or shortness of breath, and 59% of symptomless farmers. A low level of FEV1 was associated with the number of years in pig farming and bronchial hyperreactivity in group II but not group I or III. Most of the bronchial hyperreactivity was explained in the multiple regression analysis by a low FEV1, though this was significant only for farmers in group II. Thus farmers who reported asthma, wheezing, shortness of breath, or a dry cough in general had airways obstruction with an increased residual volume, whereas symptomless farmers had normal lung function. Severe bronchial hyperreactivity was mostly explained by a diagnosis of asthma and poor lung function, though some farmers with normal lung function and no respiratory symptoms had increased bronchial reactivity. PMID:2799744

  20. Endoscopic bronchial occlusion with silicone spigots under virtual bronchoscopic navigation

    Science.gov (United States)

    Sato, Shingo; Shiroyama, Takayuki; Nishida, Takuji; Nishihara, Takashi; Okamoto, Norio

    2016-01-01

    Abstract A 68‐year‐old woman with interstitial lung disease related to dermatomyositis and systemic scleroderma was admitted to our hospital with fever and dyspnoea. Although the fever was reduced after antibiotic therapy, a left pneumothorax suddenly occurred on day 27 after admission. A continuous air leak persisted despite chest drainage with three tubes and repeated pleurodesis. Chest computed tomography (CT) images showed a cavitary lesion with a pinhole in the left upper division, which was suspected to be the affected lesion with the air leak. Virtual bronchoscopic navigation images were constructed from CT data. Bronchial occlusion with Endobronchial Watanabe Spigots (EWSs) was performed on day 52. Two medium‐sized EWSs were inserted into the left B1 + 2a and B1 + 2b, and the air leak stopped immediately. No procedure‐related adverse events occurred. All three chest tubes were successfully removed by day 60. This case demonstrates that virtual bronchoscopic navigation can improve bronchial occlusion procedures using EWSs. PMID:27512560

  1. 支气管哮喘患者自我管理能力与病情控制水平相关性研究%Study for the correlation between bronchial asthma self-management skills and disease control level

    Institute of Scientific and Technical Information of China (English)

    韦春; 戴霞; 王自秀; 刘剑梅; 王昕云

    2015-01-01

    Objective To study the relationship between self‐management capability and disease control level for patients with bronchial asthma for providing theoretical foundation for self‐management education and prevent and control bronchial asthma effectively .Method 170 patients with bronchial asthma during non‐acute attack period were investigated by general information questionnaire and asthma self‐management ability questionnaire from June 15 , 2014 to November 15 ,2014 with the purpose of obtaining patients’ medical history and symptom ,and then graded the level of asthma control according to patients’ daytime symptom ,physical limitation ,night symptom ,use of abi‐rritant every week ,forced expiratory volume 1 seconds (FEV1) and number of acute onset .Result The self‐manage‐ment ability score of bronchial asthma patients is (77 .070 ± 13 .891) .The full control number of bronchial asthma patients was 68(40 .0% ) ,the partial control number is 39(22 .9% ) ,and the uncontrolled number is 63(37 .1% ) . The self‐management capability and disease control level have positive correlation (R=0 .843 ,Pconsult(R=0 .660)>self‐monitoring(R=0 .640)>avoiding triggers(R=0 .601)>the identification and treatment of symptoms (R=0 .404) .Conclusion With the improvement of self‐management a‐bility for patients with bronchial asthma ,the disease control level is increased correspondingly .%目的:探讨支气管哮喘患者自我管理能力与病情控制水平的关系,为支气管哮喘患者自我管理教育及有效预防、控制支气管哮喘提供理论依据。方法采用一般资料调查表、哮喘自我管理能力调查问卷于2014年6月15日-2014年11月15日对170例非急性发作期的支气管哮喘患者进行问卷调查,采集病史、症状,并由医生根据患者的日间症状、活动受限情况、夜间症状、每周使用缓解药次数、第1秒用力呼气量(FEV1)、急性发作次数对哮喘控制水平进

  2. Low power laser therapy in treatment of bronchial asthma

    Directory of Open Access Journals (Sweden)

    Milojević Momir

    2003-01-01

    Full Text Available Introduction Modern concept of acupuncture is based on the fact there are designated locations on the surface of human body, which are related to integrative systems of an organism by means of sensory nerves, correlating and synchronizing organ functioning, depending on external and internal conditions, by means of nervous and neurohumoral regulation of metabolic and regenerative processes, including also mobilization of immunological, protective and antistress reactions. Apart from standard needle acupuncture, other methods of stimulating acupuncture points are also applied. Due to invention of low power lasers, irradiation laser acupuncture has been introduced into routine medical practice, characterized by painless and aseptic technique and outstanding clinical results. Material and methods The investigation was aimed at defining therapeutic effects of low power laser irradiation by stimulating acupuncture points or local treatment of asthma. A prospective analysis included 50 patients treated at the Institute of Pulmonary Diseases in Sremska Kamenica during 2000, 2001 and 2002. Together with conservative treatment of present disease, these patients were treated with laser stimulation of acupuncture points in duration of ten days. During treatment changes of functional respiratory parameters were recorded. Results were compared with those in the control group. The control group consisted of the same number of patients and differed from the examination group only by not using laser stimulation. Results Patients with bronchial asthma presented with significant improvement (p<0,0005 of all estimated lung function parameters just 30 minutes after laser stimulation. Improvements achieved on the third and the tenth day of treatment were significantly higher (p<0,001 to p<0,00005 in the examination group in comparison with the control group. Further investigation confirmed that improvement of measured lung function parameters was significantly

  3. Deposition of aerosols and bronchial clearance measurements

    International Nuclear Information System (INIS)

    A special inhalative device is described for reproducible deposition patterns of radioactive aerosols to measure mucociliary and tussive clearance and to evaluate the effect of drugs on the bronchial tree is described. Additive actions on mucus transport exist between β2-agonists and theophylline, but not incombination with inhalative quarternary ammonium compounds (ipatropium and oxitropium bromide). Mucolytics are generally less effective on mucociliary clearance than β2-agonists and theophylline, positive, negative and nonresponders are ofter seen due to the different viscoelastic properties of the mucus. Mucus transport is more than mucociliary clearance. Two-phase gas/liquid movement and coughing are also important transport mechanisms for bronchial mucus. Therefore, bronchodilators enhance mucus transport by increasing airway patency, which increases total and regional air flow and improves cough clearance. (orig.)

  4. Bronchial thermoplasty in asthma: current perspectives

    Directory of Open Access Journals (Sweden)

    Laxmanan B

    2015-05-01

    Full Text Available Balaji Laxmanan, D Kyle Hogarth Section of Pulmonary and Critical Care Medicine, University of Chicago Medicine, Chicago, IL, USA Abstract: Bronchial thermoplasty (BT is a novel therapy for patients with severe asthma. Using radio frequency thermal energy, it aims to reduce the airway smooth muscle mass. Several clinical trials have demonstrated improvements in asthma-related quality of life and a reduction in the number of exacerbations following treatment with BT. In addition, recent data has demonstrated the long-term safety of the procedure as well as sustained improvements in rates of asthma exacerbations, reduction in health care utilization, and improved quality of life. Further study is needed to elucidate the underlying mechanisms that result in these improvements. In addition, improved characterization of the asthma subphenotypes likely to exhibit the largest clinical benefit is a critical step in determining the precise role of BT in the management of severe asthma. Keywords: bronchial thermoplasty, severe asthma, airway smooth muscle

  5. Relationship Between Atopy and Bronchial Hyperresponsiveness

    OpenAIRE

    Suh, Dong In; Koh, Young Yull

    2013-01-01

    Both atopy and bronchial hyperresponsiveness (BHR) are characteristic features of asthma. They are also found among non-asthmatic subjects, including allergic rhinitis patients and the general population. Atopy and BHR in asthma are closely related. Atopy induces airway inflammation as an IgE response to a specific allergen, which causes or amplifies BHR. Moreover, significant evidence of the close relationship between atopy and BHR has been found in non-asthmatic subjects. In this article, w...

  6. Bronchial reactions to exposure to welding fumes.

    OpenAIRE

    Contreras, G R; Chan-Yeung, M.

    1997-01-01

    OBJECTIVES: To study the airway response and its mechanism to welding fumes in six welders with respiratory symptoms. METHODS: Methacholine and welding challenge tests were carried out. The concentration of welding fumes during the exposure test was measured. On two subjects who developed bronchoconstricition to welding challenge, additional tests were carried out including prick, patch, and inhalation challenges with metal salt solutions. RESULTS: Three subjects developed immediate bronchial...

  7. Bronchial stenosis after tuberculosis - a case report

    International Nuclear Information System (INIS)

    The authors report a case of bronchial stenosis after pulmonary tuberculosis infection in a 46 year-old man presenting symptoms related to upper left lobe collapse, that was identified by chest roentgenograms. The evaluation by computed tomography showed narrowing of the left main bronchus and obliteration of the upper left bronchus. The patient had a postero-lateral thoracotomy, lower left bronchus and left main bronchus bronchoplasty, with upper left lobectomy. (author)

  8. Bronchiolitis obliterans organising pneumonia simulating bronchial carcinoma

    Energy Technology Data Exchange (ETDEWEB)

    Murphy, J.; Flower, C. [Department of Radiology, Addenbrooke`s Hospital, University of Cambridge Teaching Hospital (United Kingdom); Schnyder, P. [Department of Radiology, University Hospital, Lausanne (Switzerland); Herold, C. [Department of Radiology, University Hospital of Vienna (Austria)

    1998-09-01

    Idiopathic bronchiolitis obliterans organising pneumonia (BOOP) is an uncommon but well-recognised condition that usually presents radiologically as bilateral multifocal patchy areas of consolidation on the chest radiograph and on computed tomography (CT). Five cases are described in which the presenting feature was that of a solitary pulmonary nodule. Four of these nodules showed evidence of cavitation and three patients presented with haemoptysis. In all cases the appearances closely resembled bronchial carcinoma. (orig.) With 5 figs., 21 refs.

  9. Dynamic Properties of Human Bronchial Airway Tissues

    CERN Document Server

    Wang, Jau-Yi; Pallai, Prathap; Corrigan, Chris J; Lee, Tak H

    2011-01-01

    Young's Modulus and dynamic force moduli were measured on human bronchial airway tissues by compression. A simple and low-cost system for measuring the tensile-strengh of soft bio-materials has been built for this study. The force-distance measurements were undertaken on the dissected bronchial airway walls, cartilages and mucosa from the surgery-removed lungs donated by lung cancer patients with COPD. Young's modulus is estimated from the initial slope of unloading force-displacement curve and the dynamic force moduli (storage and loss) are measured at low frequency (from 3 to 45 Hz). All the samples were preserved in the PBS solution at room temperature and the measurements were perfomed within 4 hours after surgery. Young's modulus of the human bronchial airway walls are fond ranged between 0.17 and 1.65 MPa, ranged between 0.25 to 1.96 MPa for cartilages, and between 0.02 to 0.28 MPa for mucosa. The storage modulus are found varying 0.10 MPa with frequency while the loss modulus are found increasing from ...

  10. Eosinophils promote epithelial to mesenchymal transition of bronchial epithelial cells.

    Directory of Open Access Journals (Sweden)

    Atsushi Yasukawa

    Full Text Available Eosinophilic inflammation and remodeling of the airways including subepithelial fibrosis and myofibroblast hyperplasia are characteristic pathological findings of bronchial asthma. Epithelial to mesenchymal transition (EMT plays a critical role in airway remodelling. In this study, we hypothesized that infiltrating eosinophils promote airway remodelling in bronchial asthma. To demonstrate this hypothesis we evaluated the effect of eosinophils on EMT by in vitro and in vivo studies. EMT was assessed in mice that received intra-tracheal instillation of mouse bone marrow derived eosinophils and in human bronchial epithelial cells co-cultured with eosinophils freshly purified from healthy individuals or with eosinophilic leukemia cell lines. Intra-tracheal instillation of eosinophils was associated with enhanced bronchial inflammation and fibrosis and increased lung concentration of growth factors. Mice instilled with eosinophils pre-treated with transforming growth factor(TGF-β1 siRNA had decreased bronchial wall fibrosis compared to controls. EMT was induced in bronchial epithelial cells co-cultured with human eosinophils and it was associated with increased expression of TGF-β1 and Smad3 phosphorylation in the bronchial epithelial cells. Treatment with anti-TGF-β1 antibody blocked EMT in bronchial epithelial cells. Eosinophils induced EMT in bronchial epithelial cells, suggesting their contribution to the pathogenesis of airway remodelling.

  11. PROSPECTIVE STUDY OF VITAMIN D DEFICIENCY IN CHILDREN WITH BRONCHIAL ASTHMA AND ITS SEVERITY

    OpenAIRE

    Senthil Kumar; Sasikumar; Theenathayalan

    2015-01-01

    BACKGROUND Bronchial asthma is one of the most common chronic respiratory diseases in children worldwide. Though the cause is multifactorial due to various genetic and environmental factors, the hypothesis that link between the rising asthma prevalence and low Vitamin D levels has evolved. There are conflicting reports between studies on Vitamin D deficiency and prevalence of asthma in children. Due to the conflicting results and high prevalence of both asthma and Vitamin D de...

  12. Nasal and bronchial airway reactivity in allergic and non allergic airway inflammation

    OpenAIRE

    Kölbeck, Karl-Gustav

    2003-01-01

    In allergic or asthmatic airways disease, upper and lower airways show a uniform eosinophilic inflammation of the mucosa, and bronchial hyperreactivity is a common finding. To study the co- variation of mucosal reactivity in upper and lower airways, histamine challenges of both sites were performed in a group of patients with allergic rhinitis during non-season. Upper airways were monitored during challenge by the use of rhinostereometry, an optical technique that non-invasi...

  13. [Ground-based speleo-climatic wards and the experience with their use in bronchial asthma].

    Science.gov (United States)

    Krasnoshteĭn, A E; Barannikov, V G; Shchekotov, V V; Lebedeva, T M; Bitinskaia, L N; Khasanova, R B; Surovtseva, M V; Mezentseva, E V; Isaevich, A G; Lysenko, D I

    1999-01-01

    Ambient air in ground wards made to contain natural potassium-sodium salt is close in characteristics to the atmosphere of underground wards but has a number of specificities: daily fluctuations of concentrations of light aeroions and aerosol particles, elevated concentration of light aeroions concentration, low radiation background, minimal bacterial contamination. The stay in ground wards brings great benefit for patients with bronchial asthma. Ground speleo-climatic wards are perspective in nonpharmacological treatment of patients with allergic respiratory diseases. PMID:10429568

  14. Pulmonary vascular-bronchial interactions: acute reduction in pulmonary blood flow alters lung mechanics

    OpenAIRE

    Schulze-Neick, I; Penny, D; Derrick, G; Dhillon, R; Rigby, M.; Kelleher, A.; Bush, A; Redington, A

    2000-01-01

    BACKGROUND—Postoperative pulmonary hypertension in children after congenital heart surgery is a risk factor for death and is associated with severe acute changes in both pulmonary vascular resistance and lung mechanics.
OBJECTIVE—To examine the impact of changes in pulmonary blood flow on lung mechanics in preoperative children with congenital heart disease, in order to assess the cause-effect relation of pulmonary vascular-bronchial interactions.
DESIGN—Prospective, cross sectional study.
SE...

  15. Bronchial smooth muscle remodeling involves calcium-dependent enhanced mitochondrial biogenesis in asthma

    OpenAIRE

    Trian, Thomas; Benard, Giovanni; Begueret, Hugues; Rossignol, Rodrigue; Girodet, Pierre-Olivier; Ghosh, Debajyoti; Ousova, Olga; Vernejoux, Jean-Marc; Marthan, Roger; Tunon-de-Lara, José-Manuel; Berger, Patrick

    2007-01-01

    Asthma and chronic obstructive pulmonary disease (COPD) are characterized by different patterns of airway remodeling, which all include an increased mass of bronchial smooth muscle (BSM). A remaining major question concerns the mechanisms underlying such a remodeling of BSM. Because mitochondria play a major role in both cell proliferation and apoptosis, we hypothesized that mitochondrial activation in BSM could play a role in this remodeling. We describe that both the mitochondrial mass and ...

  16. COMPARATIVE EFFICACY OF INHALED DRUGS FORMOTEROL / BECLOMETHASONE AND FORMOTEROL / BUDESONIDE AND BRONCHIAL ASTHMA

    OpenAIRE

    Nikitin, V. A.; O. A. Lepuchova; L. V. Vasilieva; E. P. Karpuchina

    2016-01-01

    There were examined 86 patients aged 20 to 65 years with a diagnosis of bronchial asthma (BA). The objective is to evaluate the level of disease control, to study the effectiveness, safety and usability when used Foster (beclomethasone dipropionate and formoterol fumarate fixed combination) Combination and Foradil (formoterol fumarate and budesonide in free combination). Patients were divided into 2 groups randomly. The first group received Foster (beclomethasone dipropionate and formoterol f...

  17. Content of the Nitrogen Oxide Metabolites in a Condensate of Exhaling Air in Children with a Bronchial Asthma Control Different Level

    Directory of Open Access Journals (Sweden)

    Eliseeva T.I.

    2010-12-01

    Full Text Available Aim of investigation is establishment of regularity of the nitrogen oxide metabolite content change in the exhaling air condensate (EAC in children with a bronchial asthma control different level, assessed with a use of the ACQ day and week scales (Asthma Control Questionnaire. Materials and Methods. A summarized content of nitrites and nitrates in the exhaling air condensate in 61 infants with a bronchial asthma control different level is analyzed; it is analyzed in a disease dynamics in a part of them. Results. A summarized content of the nitrogen oxide metabolites in the EAC in patients with an absolute and full control of a bronchial asthma, not receiving the glucocorticosteroid therapy, was trustworthy higher than in patients, receiving that therapy. A trustworthy dependence between a summarized content of the nitrogen oxide metabolites in the EAC and a level of a bronchial asthma control in patients, receiving a similar therapy (with the glucocorticosteroid inclusion, is established.

  18. Method of diagnosis of chronic non-specific diseases of lungs in children

    International Nuclear Information System (INIS)

    Method of diagnosis of chronic nonspecific diseases of lungs in children using bronchography and arteriography is suggested to improve diagnosis accuracy. The method lies in simultaneous contrasting of all bronchial arteries of both lungs. The suggested method of diagnosis enabled to obtain data on pathology of bronchial arteries and bronchial structurs, to reveal additional information about propogation and character of pathologic process

  19. Functional Metagenomics of the Bronchial Microbiome in COPD.

    Directory of Open Access Journals (Sweden)

    Laura Millares

    Full Text Available The course of chronic obstructive pulmonary disease (COPD is frequently aggravated by exacerbations, and changes in the composition and activity of the microbiome may be implicated in their appearance. The aim of this study was to analyse the composition and the gene content of the microbial community in bronchial secretions of COPD patients in both stability and exacerbation. Taxonomic data were obtained by 16S rRNA gene amplification and pyrosequencing, and metabolic information through shotgun metagenomics, using the Metagenomics RAST server (MG-RAST, and the PICRUSt (Phylogenetic Investigation of Communities by Reconstruction of Unobserved States programme, which predict metagenomes from 16S data. Eight severe COPD patients provided good quality sputum samples, and no significant differences in the relative abundance of any phyla and genera were found between stability and exacerbation. Bacterial biodiversity (Chao1 and Shannon indexes did not show statistical differences and beta-diversity analysis (Bray-Curtis dissimilarity index showed a similar microbial composition in the two clinical situations. Four functional categories showed statistically significant differences with MG-RAST at KEGG level 2: in exacerbation, Cell growth and Death and Transport and Catabolism decreased in abundance [1.6 (0.2-2.3 vs 3.6 (3.3-6.9, p = 0.012; and 1.8 (0-3.3 vs 3.6 (1.8-5.1, p = 0.025 respectively], while Cancer and Carbohydrate Metabolism increased [0.8 (0-1.5 vs 0 (0-0.5, p = 0.043; and 7 (6.4-9 vs 5.9 (6.3-6.1, p = 0.012 respectively]. In conclusion, the bronchial microbiome as a whole is not significantly modified when exacerbation symptoms appear in severe COPD patients, but its functional metabolic capabilities show significant changes in several pathways.

  20. Functional Metagenomics of the Bronchial Microbiome in COPD.

    Science.gov (United States)

    Millares, Laura; Pérez-Brocal, Vicente; Ferrari, Rafaela; Gallego, Miguel; Pomares, Xavier; García-Núñez, Marian; Montón, Concepción; Capilla, Silvia; Monsó, Eduard; Moya, Andrés

    2015-01-01

    The course of chronic obstructive pulmonary disease (COPD) is frequently aggravated by exacerbations, and changes in the composition and activity of the microbiome may be implicated in their appearance. The aim of this study was to analyse the composition and the gene content of the microbial community in bronchial secretions of COPD patients in both stability and exacerbation. Taxonomic data were obtained by 16S rRNA gene amplification and pyrosequencing, and metabolic information through shotgun metagenomics, using the Metagenomics RAST server (MG-RAST), and the PICRUSt (Phylogenetic Investigation of Communities by Reconstruction of Unobserved States) programme, which predict metagenomes from 16S data. Eight severe COPD patients provided good quality sputum samples, and no significant differences in the relative abundance of any phyla and genera were found between stability and exacerbation. Bacterial biodiversity (Chao1 and Shannon indexes) did not show statistical differences and beta-diversity analysis (Bray-Curtis dissimilarity index) showed a similar microbial composition in the two clinical situations. Four functional categories showed statistically significant differences with MG-RAST at KEGG level 2: in exacerbation, Cell growth and Death and Transport and Catabolism decreased in abundance [1.6 (0.2-2.3) vs 3.6 (3.3-6.9), p = 0.012; and 1.8 (0-3.3) vs 3.6 (1.8-5.1), p = 0.025 respectively], while Cancer and Carbohydrate Metabolism increased [0.8 (0-1.5) vs 0 (0-0.5), p = 0.043; and 7 (6.4-9) vs 5.9 (6.3-6.1), p = 0.012 respectively]. In conclusion, the bronchial microbiome as a whole is not significantly modified when exacerbation symptoms appear in severe COPD patients, but its functional metabolic capabilities show significant changes in several pathways.

  1. Radioaerosol inhalation lung scintigraphy in bronchial asthma

    International Nuclear Information System (INIS)

    A study on obstructive changes in airways and mucociliary clearance in children and youth with bronchial asthma was performed. Radioaerosol inhalation lung scintigraphies using 99Tc-human serum albumin (HSA) were applied to 50 children and youth with bronchial asthma. The deposition patterns of the radioaerosol and aerosol clearance curves were evaluated. Abnormal deposition patterns, which consisted of non-homogeneous distribution and/or hot spot formation, were likely to be seen in patients with asthmatic attacks at the time of measurements. However, a few asymptomatic patients also revealed abnormal deposition patterns. The deposition patterns were related to FEV1.0%, MMF, V50 and V25, but especially to FEV1.0%. As an index of mucociliary clearance, β, the rate constant of the 99mTc-HSA aerosol clearance curve, was introduced. β was significantly lower in patients with abnormal aerosol deposition patterns than in normal persons. β was also significantly lower in patients undergoing asthmatic attack at the time of the measurements than in asymptomatic patients. β correlated negatively with FEV1.0%, MMF, V50 and V25, but especially with FEV1.0%. Although patients with long term affection or moderate-to-severe asthma tended to reveal abnormal deposition patterns and had low β values, these differences were not statistically significant. Radioaerosol inhalation lung scintigraphy with 99mTc-HSA is useful for evaluating not only obstructive changes in the airways but also for evaluating mucociliary clearance in children with bronchial asthma. (author)

  2. BRONCHOSCOPIC THERAPY IN PATIENTS WITH INTRALUMINAL TYPICAL BRONCHIAL CARCINOID

    NARCIS (Netherlands)

    SUTEDJA, TG; SCHREURS, AJ; VANDERSCHUEREN, RG; KWA, B; VANDERWERF, TS; POSTMUS, PE

    1995-01-01

    Objective: To study the efficacy of bronchoscopic therapy in patients with intraluminal typical bronchial carcinoid. Design: Retrospective analysis of the data of patients with bronchial carcinoid, treated primarily with bronchoscopic techniques such as Nd-YAG laser in various hospitals in the Nethe

  3. Increased resting bronchial tone in normal subjects acclimatised to altitude

    OpenAIRE

    Wilson, C.; Bakewell, S; M. Miller; Hart, N; McMorrow, R; BARRY, P.; Collier, D; Watt, S; Pollard, A.

    2002-01-01

    Background: Normal subjects frequently experience troublesome respiratory symptoms when acclimatised to altitude. Bronchial hyperresponsiveness (BHR) and full and partial flow-volume loops were measured before and after ascent to 5000 m altitude to determine if there are changes in resting bronchial tone and BHR that might explain the symptoms.

  4. [Physical exercise and bronchial asthma].

    Science.gov (United States)

    Endre, László

    2016-06-26

    An article was published in the Lancet in 1935 about the therapy of asthmatic patients, using a special breathing exercise (the authors used a control group, too). Swimming, as a complementary therapy for asthmatic children, was first recommended in 1968, by authors from the United States. In Hungary, regular swimming training for asthmatic children is in use since August, 1981. As the result of this exercise, the physical fitness of asthmatic children (using this method regularly for years) increased dramatically, and it is much better compared to that found in the non asthmatic, non swimming children of the same age group. The requirement for asthma medication decreased, and the severity of their disease significantly decreased, also. On the other hand, asthma is not a rarity even among elite athletes. It is most frequent in the endurance sports (for example in Northern Europe among cross-country skiers its prevalence is between 14-54%, among long distance runners 15-24%, and among swimmers 13-44%). The possible reason is related to the fact that elite athletes inspire 200 liter air/minute (mostly through the mouth). Air pollution and allergens can penetrate in the lower respiratory tract. The air causes cooling and drying of the mucosa of the airways and, as a consequence, mediators are liberated which produce oedema of the mucosa, and bronchoconstriction. Beta-2-receptor agonists inhalation can prevent (or decrease significantly) this phenomenon. These agents are used regularly by elite athletes, too. The non-medical possibilities for prevention include wearing a special mask, frequent ventilation of the swimming pool's air, consumption of omega-3-fatty acid, and inhalation of dry salt (very small, and very clear sodiumchloride particles).

  5. [Physical exercise and bronchial asthma].

    Science.gov (United States)

    Endre, László

    2016-06-26

    An article was published in the Lancet in 1935 about the therapy of asthmatic patients, using a special breathing exercise (the authors used a control group, too). Swimming, as a complementary therapy for asthmatic children, was first recommended in 1968, by authors from the United States. In Hungary, regular swimming training for asthmatic children is in use since August, 1981. As the result of this exercise, the physical fitness of asthmatic children (using this method regularly for years) increased dramatically, and it is much better compared to that found in the non asthmatic, non swimming children of the same age group. The requirement for asthma medication decreased, and the severity of their disease significantly decreased, also. On the other hand, asthma is not a rarity even among elite athletes. It is most frequent in the endurance sports (for example in Northern Europe among cross-country skiers its prevalence is between 14-54%, among long distance runners 15-24%, and among swimmers 13-44%). The possible reason is related to the fact that elite athletes inspire 200 liter air/minute (mostly through the mouth). Air pollution and allergens can penetrate in the lower respiratory tract. The air causes cooling and drying of the mucosa of the airways and, as a consequence, mediators are liberated which produce oedema of the mucosa, and bronchoconstriction. Beta-2-receptor agonists inhalation can prevent (or decrease significantly) this phenomenon. These agents are used regularly by elite athletes, too. The non-medical possibilities for prevention include wearing a special mask, frequent ventilation of the swimming pool's air, consumption of omega-3-fatty acid, and inhalation of dry salt (very small, and very clear sodiumchloride particles). PMID:27319382

  6. Radioaerosol Inhalation Imaging in Bronchial Asthma

    Energy Technology Data Exchange (ETDEWEB)

    Kim, Bum Soo; Park, Young Ha; Park, Jeong Mi; Chung, Myung Hee; Chung, Soo Kyo; Shinn, Kyung Sub; Bahk, Yong Whee [Catholic University College of Medicine, Seoul (Korea, Republic of)

    1991-03-15

    Radioaerosol inhalation imaging (RII) has been used in radionuclide pulmonary studies for the past 20 years. The method is well accepted for assessing regional ventilation because of its usefulness, easy fabrication and simple application system. To evaluate its clinical utility in the study of impaired regional ventilation in bronchial asthma, we obtained and analysed RIIs in 31 patients (16 women and 15 men; age ranging 21-76 years) with typical bronchial asthma at the Department of Radiology, Kangnam St. Mary's Hospital, Catholic University Medical college, from January, 1988 to August, 1989. Scintiscans were obtained with radioaerosol produced by a HARC(Bhabha Atomic Research Center, India) nebulizer with 15 mCi of {sup 99m}Tc-phytate. The scanning was performed in anterior, posterior and lateral projections following 5-minute inhalation of radioaerosol on sitting position. The scans were analysed and correlated with the results of pulmonary function study and the findings of chest radiography. Fifteen patients had concomitant lung perfusion image with {sup 99m}Tc-MAA. Follow-up scans were obtained in 5 patients after bronchodilator therapy. 1 he patients were divided into (1) attack type (4 patients), (2) resistant type (5 patients), (3) remittent type (10 patients) and (4) bronchitic type (12 patients). Chest radiography showed hyperinflation, altered pulmonary vascularity, thickening of the bronchial wall and accentuation of hasal interstitial markings in 26 of the 31 patients. Chest radiographs were normal in the remaining 5 patients. Regardless of type, the findings of RII were basically the same, and characterized by the deposition of radioaerosol in the central parts or in the main respiratory air ways along with mottled nonsegmental ventilation defects in the periphery. Peripheral parenchymal defects were more extensive than that of expected findings from clinical symptoms, pulmonary function test and chest radiograph. Broomstick sign was present

  7. Radioaerosol Inhalation Imaging in Bronchial Asthma

    International Nuclear Information System (INIS)

    Radioaerosol inhalation imaging (RII) has been used in radionuclide pulmonary studies for the past 20 years. The method is well accepted for assessing regional ventilation because of its usefulness, easy fabrication and simple application system. To evaluate its clinical utility in the study of impaired regional ventilation in bronchial asthma, we obtained and analysed RIIs in 31 patients (16 women and 15 men; age ranging 21-76 years) with typical bronchial asthma at the Department of Radiology, Kangnam St. Mary's Hospital, Catholic University Medical college, from January, 1988 to August, 1989. Scintiscans were obtained with radioaerosol produced by a HARC(Bhabha Atomic Research Center, India) nebulizer with 15 mCi of 99mTc-phytate. The scanning was performed in anterior, posterior and lateral projections following 5-minute inhalation of radioaerosol on sitting position. The scans were analysed and correlated with the results of pulmonary function study and the findings of chest radiography. Fifteen patients had concomitant lung perfusion image with 99mTc-MAA. Follow-up scans were obtained in 5 patients after bronchodilator therapy. 1 he patients were divided into (1) attack type (4 patients), (2) resistant type (5 patients), (3) remittent type (10 patients) and (4) bronchitic type (12 patients). Chest radiography showed hyperinflation, altered pulmonary vascularity, thickening of the bronchial wall and accentuation of hasal interstitial markings in 26 of the 31 patients. Chest radiographs were normal in the remaining 5 patients. Regardless of type, the findings of RII were basically the same, and characterized by the deposition of radioaerosol in the central parts or in the main respiratory air ways along with mottled nonsegmental ventilation defects in the periphery. Peripheral parenchymal defects were more extensive than that of expected findings from clinical symptoms, pulmonary function test and chest radiograph. Broomstick sign was present in 1.7 patients

  8. Radiological Management of Hemoptysis: A Comprehensive Review of Diagnostic Imaging and Bronchial Arterial Embolization

    International Nuclear Information System (INIS)

    Hemoptysis can be a life-threatening respiratory emergency and indicates potentially serious underlying intrathoracic disease. Large-volume hemoptysis carries significant mortality and warrants urgent investigation and intervention. Initial assessment by chest radiography, bronchoscopy, and computed tomography (CT) is useful in localizing the bleeding site and identifying the underlying cause. Multidetector CT angiography is a relatively new imaging technique that allows delineation of abnormal bronchial and nonbronchial arteries using reformatted images in multiple projections, which can be used to guide therapeutic arterial embolization procedures. Bronchial artery embolization (BAE) is now considered to be the most effective procedure for the management of massive and recurrent hemoptysis, either as a first-line therapy or as an adjunct to elective surgery. It is a safe technique in the hands of an experienced operator with knowledge of bronchial artery anatomy and the potential pitfalls of the procedure. Recurrent bleeding is not uncommon, especially if there is progression of the underlying disease process. Prompt repeat embolization is advised in patients with recurrent hemoptysis in order to identify nonbronchial systemic and pulmonary arterial sources of bleeding. This article reviews the pathophysiology and causes of hemoptysis, diagnostic imaging and therapeutic options, and technique and outcomes of BAE.

  9. Alternative spliced CD1d transcripts in human bronchial epithelial cells.

    Directory of Open Access Journals (Sweden)

    Kambez Hajipouran Benam

    Full Text Available CD1d is a MHC I like molecule which presents glycolipid to natural killer T (NKT cells, a group of cells with diverse but critical immune regulatory functions in the immune system. These cells are required for optimal defence against bacterial, viral, protozoan, and fungal infections, and control of immune-pathology and autoimmune diseases. CD1d is expressed on antigen presenting cells but also found on some non-haematopoietic cells. However, it has not been observed on bronchial epithelium, a site of active host defence in the lungs. Here, we identify for the first time, CD1D mRNA variants and CD1d protein expression on human bronchial epithelial cells, describe six alternatively spliced transcripts of this gene in these cells; and show that these variants are specific to epithelial cells. These findings provide the basis for investigations into a role for CD1d in lung mucosal immunity.

  10. Serum levels of total IgE and soluble CD23 in bronchial asthma

    Directory of Open Access Journals (Sweden)

    G. Di Lorenzo

    1996-01-01

    Full Text Available The aim of the present study was to compare, during the pollen season, serum levels of total IgE and soluble CD23 (sCD23 from patients with allergic bronchial asthma, with those from healthy subjects. Significantly higher levels of total IgE and sCD23 were found in patients with asthma compared to the control group. Both in normal controls and in asthmatic patients, a significant correlation was shown between the levels of these two molecules. In asthmatic patients, significant correlations were found for both total IgE and sCD23, with lung function measured as bronchial responsiveness to inhaled methacholine. These results suggest that in asthmatic patients, in addition to the study of total serum IgE levels, the assessment of sCD23 serum levels may be helpful in the evaluation of disease activity.

  11. Usefulness of antioxidant drugs in bronchial asthma

    International Nuclear Information System (INIS)

    Bronchial asthma is a clinical syndrome with possible correlation to oxidative stress, therefore the effectiveness of some antioxidant drugs has been studied in management of chronic bronchial asthma. Methods: This study was carried out in the Al- Kadhimia Teaching Hospital between December 2008 to May 2009 on 56 patients of both sexes who were randomly allocated to 7 groups, plus 10 healthy volunteers as control group. Each group was given one of the following drugs: vitamin E, vitamin C, combination of vitamin E and C, selenium, zinc, allopurinol and garlic oil, in addition to their classical treatment of asthma and their pulmonary function tests were conducted as well as measuring the levels of serum zinc, calcium, and malondialdehyde (MDA) before and after treatment. Results: All asthmatic patients were suffering from oxidative stress and this was detected by measuring the level of serum MDA which was 2-3 folds more than the control group, and all antioxidants except allopurinol showed a beneficial effect of different degrees in the pulmonary function tests accompanied with clinical improvement of patients' condition and marked decrease in the number of daily attacks. Antioxidants can compensate the oxidative stress that correlates with asthma, can reduce the symptoms of asthma, and improve pulmonary functions. (author)

  12. Detection of trisomy 7 in bronchial cells from uranium miners

    Energy Technology Data Exchange (ETDEWEB)

    Lechner, J.F.; Neft, R.E.; Belinsky, S.A. [and others

    1995-12-01

    New Mexico was the largest producer of uranium in the western world during 1960s and 1970s. Investigators at the University of New Mexico School of Medicine`s Epidemiology and Cancer Control Program have been conducting epidemiological studies on uranium miners over the past 2 decades. Currently, this cohort includes more than 3600 men who had completed at least 1 y of underground work experience in New Mexico by December 31, 1976. These miners, who are now in their 5th through 7th decades, the age when lung cancer incidence is highest, are at high risk for developing this disease because they were exposed to high levels of radon progeny in the mines, and they also smoked tobacco. However, not all people comparably exposed develop lung cancer; in fact, the lifetime risk of lung cancer for the smoking uranium miners has been projected by epidemiological analyses to be no higher than 50%. Therefore, the identification of gene alterations in bronchial epithelium would be a valuable tool to ascertain which miners are at greatest risk for lung cancer. The underlying significance of the current effort confirms the hypothesis that chronic exposure to high concentrations of {alpha}-particles and tobacco smoke produces genetically altered lung epithelial cells throughout the respiratory tract of some susceptible individuals before they develop clinical disease.

  13. CT diagnosis of traumatic bronchial rupture in children

    International Nuclear Information System (INIS)

    Bronchial rupture is a rare and serious complication of blunt chest trauma in children. The diagnosis of this injury is challenging and requires a high degree of clinical suspicion. It is frequently associated with other severe injuries that may draw the focus of attention away from this potentially catastrophic but treatable injury. The radiographic findings of bronchial rupture have been reported in very few series. We report the findings in two children with bronchial rupture diagnosed by CT, in whom CT resulted in a significant change in patient management. (orig.)

  14. Low molecular weight components of pollen alter bronchial epithelial barrier functions.

    Science.gov (United States)

    Blume, Cornelia; Swindle, Emily J; Gilles, Stefanie; Traidl-Hoffmann, Claudia; Davies, Donna E

    2015-01-01

    The bronchial epithelium plays a key role in providing a protective barrier against many environmental substances of anthropogenic or natural origin which enter the lungs during breathing. Appropriate responses to these agents are critical for regulation of tissue homeostasis, while inappropriate responses may contribute to disease pathogenesis. Here, we compared epithelial barrier responses to different pollen species, characterized the active pollen components and the signaling pathways leading to epithelial activation. Polarized bronchial cells were exposed to extracts of timothy grass (Phleum pratense), ragweed (Ambrosia artemisifolia), mugwort (Artemisia vulgaris), birch (Betula alba) and pine (Pinus sylvestris) pollens. All pollen species caused a decrease in ionic permeability as monitored trans-epithelial electrical resistance (TER) and induced polarized release of mediators analyzed by ELISA, with grass pollen showing the highest activity. Ultrafiltration showed that the responses were due to components isorhamnetin present in grass pollen contributed to the overall effect on airway epithelial barrier responses. In conclusion, bronchial epithelial barrier functions are differentially affected by several low molecular weight components released by pollen. Furthermore, ionic permeability and innate cytokine production are differentially regulated. PMID:26451347

  15. Treatment of bronchial asthma with low-level laser in attack-free period at children

    Science.gov (United States)

    Ailioaie, C.; Ailioaie, Laura

    2000-06-01

    Bronchial asthma is a common disease in both the pediatric and adult populations, characterized by wide variations over short periods of time in resistance to airflow in intrapulmonary airways. A primary goal in the use of low- level laser therapy (LLLT) was the safe, effective and rapid palliation of symptoms owing to tracheal or bronchial obstruction. We have investigated the effects of LLLT comparatively with other modality trials in children's asthma. In the study were included 98 patients aged 10-18 years diagnosed with moderate or severe asthma, in attack- free period. The patients were divided into 3 groups. Group 1 received only laser therapy using extra meridian acupuncture points and scanning technique. Group 2 was treated only with inhaled Serevent 2 X 25 micrometers , two times daily, 3 months. Group 3 was tread with Theophylline retard in dosage of 15-mg/kg/12 h, 3 months. At the end of treatment we remarked a noticeable improvement of the clinical, functional and immunological characteristics at 83 percent of patients in group 1, comparatively with only 70 percent (group 2) and 53 percent (group 3). The LLLT had a very good action on bronchial patency , displayed an immunocorrecting action and is recommended in attack-free periods at children.

  16. Epidemiology of bronchial asthma and diabetes mellitus in children and teenagers of Ozyorsk town situated in the area of mayak nuclear enter-prise supervision zone

    International Nuclear Information System (INIS)

    The epidemiology of bronchial asthma and diabetes mellitus in children and teenagers living in Ozyorsk town, which is situated in Mayak nuclear enterprise supervision zone, was studied. By the medical and statistical data in Ozyorsk for the studying forms of multifactorial diseases the trends to the increase both for primary incidence and prevalence were marked. By using the genetic and epidemiological analysis we marked the higher ratio of accumulated incidence as a probability to fall ill with multifactorial diseases (bronchial asthma and diabetes mellitus) in population of Ozyorsk city till 20 years old in comparison with Moscow population

  17. Bronchial asthma: correlation of high resolution computerized tomography findings with clinical data; Asma bronquica: correlacao de achados em tomografia computadorizada de alta resolucao com dados clinicos

    Energy Technology Data Exchange (ETDEWEB)

    Mogami, Roberto [Universidade do Estado, Rio de Janeiro, RJ (Brazil). Servico de Radiologia; Marchiori, Edson [Universidade Federal Fluminense, Niteroi, RJ (Brazil). Dept. de Radiologia; Kirk, Kennedy; Capone, Domenico [Universidade do Estado, Rio de Janeiro, RJ (Brazil). Servico de Pneumologia; Daltro, Pedro [Instituto Fernandes Figueira, Rio de Janeiro, RJ (Brazil). Servico de Radiologia

    1999-04-01

    In this work we did a sectional study of 31 asthmatic patients with several levels of disease severity, which were submitted to high resolution computed tomography of the thorax and spirometry, between the months of July, 1995 and August, 1997. The tomographic findings were correlated with the clinical classification of the patients and the most frequent tomographic findings were bronchial wall thickening, bronchial dilatation, air trapping, centrilobular opacities, cicatricial linear shadows, mucoid impaction, emphysema and atelectasis. In asthmatic patients of long duration we observed small airway disease and irreversible lesions as the predominant findings. In smoking patients there was no high frequency of emphysema. (author)

  18. Effect of Air Pollution, Contamination and High Altitude on Bronchial Asthma

    Directory of Open Access Journals (Sweden)

    Nesriene El margoushy*, Mohamad El Nashar**, Hatem Khairy*, Nihad El Nashar*, Hala Mohamad

    2013-01-01

    , associated with a high rate of rhinitis symptoms and hay fever. In addition to bronchial asthma, prevalence of allergic diseases in a sample of Taif citizens assessed by an original Arabic questionnaire (phase I evidenced a high prevalence of allergic diseases as Urticaria, allergic rhinitis with or without other co-morbidities, and atopic dermatitis. Effect of high altitude on bronchial asthma is controversial; at high altitudes, the concentrations of the allergens are reduced due to the reduced amounts of vegetation, animal populations and human influences, high UV light exposure and low humidity could be contributing factors to the benefits of high altitude other than allergen avoidance. On the contrary, Lower altitudes have significant beneficial effects for bronchial asthma patients but lessen with increasing altitudes; the mountain climate can modify respiratory function and bronchial responsiveness of asthmatic subjects. Hypoxia, hyperventilation of cold and dry air and physical exertion may worsen asthma or enhance bronchial hyper-responsiveness while a reduction in pollen and pollution may play an important role in reducing bronchial inflammation. Increasing attention has to be paid to the potential of urban air toxics to exacerbate asthma. Continued emphasis on the identification of strategies for reducing levels of urban air pollutants is warranted to reduce respiratory diseases and other diseases related to pollution. Efforts for reducing the asthma burden must focus on primary prevention to reduce the level of exposure of individuals and populations to common risk factors, particularly tobacco smoke, frequent lower respiratory infections during childhood, and environmental air pollution (indoor, outdoor, and occupational.

  19. Imaging findings of bronchial atresia in fetuses, neonates and infants

    Energy Technology Data Exchange (ETDEWEB)

    Alamo, Leonor; Meuli, Reto [University Hospital of Lausanne (CHUV) and University of Lausanne (UNIL), Department of Diagnostic and Interventional Radiology, Lausanne (Switzerland); Vial, Yvan [University Hospital of Lausanne (CHUV) and University of Lausanne (UNIL), Department of Obstetrics and Gynecology, Lausanne (Switzerland); Gengler, Carole [University Hospital of Lausanne (CHUV) and University of Lausanne (UNIL), Department of Pathology, Lausanne (Switzerland)

    2016-03-15

    Congenital lung malformations are increasingly detected before birth. However, bronchial atresia is rarely identified in utero and not always recognized in neonates. There are two types of atresia: (1) proximal, located at the level of the mainstem or the proximal lobar bronchi, which is extremely rare and usually lethal during pregnancy, causing a tremendous volume increase of the distal involved lung with secondary hypoplasia of the normal lung, and (2) peripheral, located at the segmental/subsegmental bronchial level, which may present as an isolated lesion or as part of a complex congenital malformation. Prenatal findings are mostly nonspecific. Postnatal exams show overinflated lung areas and focal bronchial dilations. The typical fluid-filled bronchoceles are not always observed in neonates but develop progressively in the first months of life. This pictorial essay describes the spectrum of imaging findings of bronchial atresia in fetuses, neonates and infants. (orig.)

  20. Imaging findings of bronchial atresia in fetuses, neonates and infants

    International Nuclear Information System (INIS)

    Congenital lung malformations are increasingly detected before birth. However, bronchial atresia is rarely identified in utero and not always recognized in neonates. There are two types of atresia: (1) proximal, located at the level of the mainstem or the proximal lobar bronchi, which is extremely rare and usually lethal during pregnancy, causing a tremendous volume increase of the distal involved lung with secondary hypoplasia of the normal lung, and (2) peripheral, located at the segmental/subsegmental bronchial level, which may present as an isolated lesion or as part of a complex congenital malformation. Prenatal findings are mostly nonspecific. Postnatal exams show overinflated lung areas and focal bronchial dilations. The typical fluid-filled bronchoceles are not always observed in neonates but develop progressively in the first months of life. This pictorial essay describes the spectrum of imaging findings of bronchial atresia in fetuses, neonates and infants. (orig.)

  1. Bronchial artery embolization in the treatment of massive hemoptysis

    International Nuclear Information System (INIS)

    Objective was to evaluate the efficacy of bronchial arteriography and bronchial artery embolization (BAE) in the management of massive hemoptysis in a developing Asian country. A retrospective review was carried out from March 2000 to March 2005 to evaluate the demographics, clinical presentation, radiographic studies, bronchoscopy results, and complications of bronchial arteriography and BAE at a tertiary care hospital in Pakistan. Fourteen patients (9males, 5 females) with a mean age of 49 years underwent bronchial arteriography and BAE for massive hemoptysis. Hemoptysis was caused by bronchiectasis (10 patients), active pulmonary tuberculosis (3 patients), and lung malignancy (one patient). A CT scan of the chest was carried out in 11 patients, which revealed bronchiectasis (8 patients), cavity with infiltrates (3 patients), and mass lesion (one patient). Bronchoscopy was performed in all patients. Bleeding lobe or segment was identified in 12 patients. Bronchial arteriography revealed hypervascularity (13 patients), bronchial artery hypertrophy (5 patients), hypervascularity with shunting (one patient), dense soft tissue staining (7 patients), extravasation of contrast (one patient) pseudoaneurysm (one patient). Bronchial artery embolization was carried out in all patients. Rebleeding occurred within 24 hours in 2 patients who underwent surgery and within one week another 2 patients who were managed with repeat BAE. The complication of embolization occurred in one patient (transverse myelitis). Thirteen patients improved and were discharged home. One patient with terminal lung carcinoma died due to cardiogenic shock secondary to acute myocardial infarction. Bronchial artery embolization is an effective method for management of massive hemoptysis in developing countries and has a low complication rate. (author)

  2. Eosinophils Promote Epithelial to Mesenchymal Transition of Bronchial Epithelial Cells

    OpenAIRE

    Yasukawa, Atsushi; Hosoki, Koa; Toda, Masaaki; Miyake, Yasushi; Matsushima, Yuki; Matsumoto, Takahiro; Boveda-Ruiz, Daniel; Gil-Bernabe, Paloma; Nagao, Mizuho; Sugimoto, Mayumi; Hiraguchi, Yukiko; Tokuda, Reiko; Naito, Masahiro; Takagi, Takehiro; D'Alessandro-Gabazza, Corina N.

    2013-01-01

    Eosinophilic inflammation and remodeling of the airways including subepithelial fibrosis and myofibroblast hyperplasia are characteristic pathological findings of bronchial asthma. Epithelial to mesenchymal transition (EMT) plays a critical role in airway remodelling. In this study, we hypothesized that infiltrating eosinophils promote airway remodelling in bronchial asthma. To demonstrate this hypothesis we evaluated the effect of eosinophils on EMT by in vitro and in vivo studies. EMT was a...

  3. Therapeutic pulmonary artery stenting for metastatic bronchial carcinoid

    OpenAIRE

    Vawdrey, Daniel B F; Fitzsimmons, Samantha; Veldtman, Gruschen R; Carpenter, John-Paul

    2013-01-01

    We present a case of a middle-aged man with a 3-month history of progressive shortness of breath and peripheral oedema. Ten years prior to this, he had undergone a left pneumonectomy for metastatic bronchial carcinoid. Clinical examination revealed significant right heart failure, supported by transthoracic echocardiography. CT pulmonary angiogram revealed the cause to be marked progression of the bronchial carcinoid causing severe external compression of right pulmonary artery (RPA). In view...

  4. Clinical significance of changes of serum IGF-II, IL-2 and SOD levels after treatment in pediatric patients with bronchial pneumonia

    International Nuclear Information System (INIS)

    Objective: To investigate the clinical significance of changes of serum IGF-II, IL-2 and SOD levels after treatment in pediatric patients with bronchial pneumonia. Methods: Serum IGF-II, IL-2 and SOD (with RIA) levels were measured in 33 pediatric patients with bronchial pneumonia both before and after treatment as well as in 35 controls. Results: Before treatment, serum IGF-II levels in the patients were significantly higher than those in controls (P0.05). Conclusion: Changes of serum IGF-II, IL-2 and SOD levels both before and after treatment could reflect the diseases status of the patients as well as the progress of diseases, and might be of prognostic importance in pediatric patients with bronchial pneumonia. (authors)

  5. Bronchial Artery Embolization for Massive Hemoptysis: a Retrospective Study

    Directory of Open Access Journals (Sweden)

    Ali Fani

    2013-05-01

    Full Text Available   Introduction: To assess the efficacy and safety of bronchial artery embolization in the treatment of massive hemoptysis.   Materials and Methods: A retrospective study on 46 patients (26 males and 20 females who were referred to the Razavi Hospital from April 2009 to May 2012 with massive hemoptysis and had bronchial artery embolization procedures. General characteristics of the patients including age, gender, etiology, and thorax computed tomograms, findings of bronchial angiographic, results of the embolization, complications related to bronchial artery embolization and clinical outcome during follow-up were reviewed. Results: The etiology included previous pulmonary tuberculosis in 20 cases, previous tuberculosis with bronchiectasis in 16 cases, bronchiectasis in 6 cases, and active pulmonary tuberculosis in one case. No identifiable causes could be detected in three patients. Moreover, massive hemoptysis was successfully and immediately controlled following the embolization procedure in all patients. One patient developed recurrent hemoptysis during one month following the procedure and was treated by re-embolization. No major procedure–related complication such as bronchial infarction was identified However none of the patientsexperienced neurological complications. Conclusion: Bronchial artery embolization is a safe and effective means of controlling massive hemoptysis and should be regarded as the first-line treatment for this condition.

  6. Indications for the use of bronchial thermoplasty in severe asthma.

    Science.gov (United States)

    Dheda, Keertan; Koegelenberg, Coenraad F N; Esmail, Aliasgar; Irusen, Elvis; Wechsler, Michael E; Niven, Rob M; Chung, Kian Fan; Bateman, Eric D

    2015-09-18

    Approximately 5% of the ~3 million asthmatics in South Africa have severe asthma that is associated with substantial morbidity, cost, absenteeism, preventable mortality, and the requirement for costly chronic medication that may be associated with significant adverse events. There is an unmet need for alternative safer and more effective interventions for severe asthma. A recently introduced option, bronchial thermoplasty (BT), imparts radiofrequency-generated heat energy to the airways to cause regression of airway smooth muscle. The effectiveness of this technique has been confirmed in randomised control trials and is now endorsed by several international guidelines, including the Global Initiative for Asthma (GINA) guideline, the British Asthma Guideline, and the UK National Institute of Clinical Excellence (NICE) guideline. We recommend BT as a potential therapeutic intervention for severe uncontrolled asthma, provided that it is performed by an experienced pulmonologist at an accredited centre and done within the broader context of appropriate management of the disease by doctors experienced in treating difficult-to-control asthma.

  7. Multipotent capacity of immortalized human bronchial epithelial cells.

    Directory of Open Access Journals (Sweden)

    Oliver Delgado

    Full Text Available While the adult murine lung utilizes multiple compartmentally restricted progenitor cells during homeostasis and repair, much less is known about the progenitor cells from the human lung. Translating the murine stem cell model to humans is hindered by anatomical differences between species. Here we show that human bronchial epithelial cells (HBECs display characteristics of multipotent stem cells of the lung. These HBECs express markers indicative of several epithelial types of the adult lung when experimentally tested in cell culture. When cultured in three different three-dimensional (3D systems, subtle changes in the microenvironment result in unique responses including the ability of HBECs to differentiate into multiple central and peripheral lung cell types. These new findings indicate that the adult human lung contains a multipotent progenitor cell whose differentiation potential is primarily dictated by the microenvironment. The HBEC system is not only important in understanding mechanisms for specific cell lineage differentiation, but also for examining changes that correlate with human lung diseases including lung cancer.

  8. Interventional therapy of refractory hemoptysis complicated with bronchial artery to pulmonary circulation shunt

    International Nuclear Information System (INIS)

    Objective: The presence of bronchial artery to pulmonary circulation shunt (BPS) is the pathologic reason for refractory hemoptysis. Those who have accepted traditional bronchial arterial embolization (BAE) treatment usually carry high risks of recurrence of hemoptysis. At the same time,because of the limits of BAE treatment, safety can not be ignored when we pay full attention to its therapeutic effect. This research aims to find out an effective method to treat the refractory hemoptysis with BPS. Methods: During the period from Sep. 1996 to Feb. 2010, two hundred and twelve patients of hemoptysis were treated with BAE. Of the total 212 patients, BPS was confirmed by DSA angiography in 99, including 72 males and 27 females with a mean age of 47.6 years. The primary diseases included bronchiectasis (n = 25), tuberculosis (n = 64) and inflammatory disorders(n = 10). All patients were treated with BAE. According to the embolization agent used in the treatment the patients were divided into pure Gelfoam group (n = 52) and permanent embolization group (n = 47, using PVA particles, coils, etc.). All patients were followed up regularly at one day, two weeks, four weeks, six months, one year, two years after the treatment. The data were analyzed by using chi square test and rank sum test. Results: Of 52 cases in permanent embolization group, complete cure was achieved in 49, excellent results in 2 and effective response in one. For patients in pure Gelfoam group, complete cure was obtained in 37, excellent results in 16, effective response in 2 and ineffectiveness in 2. Statistic analysis showed that a significant difference in therapeutic effectiveness existed between two groups (P < 0.05). Conclusion: This study clearly indicates that selective bronchial arterial permanent dual embolization is an effective technique for the treatment of refractory hemoptysis, which is definitely superior to the pure Gelfoam emboliztion in obtaining excellent therapeutic results. Besides

  9. Achievement of control of bronchial asthma at the stage of medical rehabilitation

    Directory of Open Access Journals (Sweden)

    Grygus I.M.

    2011-02-01

    Full Text Available An inspection is conducted 70 patients on intermittent bronchial asthma at the stage of intensifying. The special program of medical rehabilitation, which includes the modified methods of medical physical culture, physical therapy facilities, is offered in permanent establishment. Application of this program brought to the height of size of Asthma Control Test from 17,41±0,35 to 24,03±0,32 points over. Control of flow of disease which did not come at treatment of patients only by medicinal preparations was arrived at in all cases of application of the program of medical rehabilitation.

  10. [Pulmonary autoantibodies in bronchial asthma patients undergoing cave and climate therapy in Bystrá].

    Science.gov (United States)

    Pasková, S; Kolesár, J; Siposová, E

    1976-01-01

    Pulmonary autoantibodies were determined by us in the sera of 37 patients who underwent a 6-week speleo- and climatotherapy in the cave of Bystrá. The titres found in both groups of patients showed no difference. Global evaluation revealed a high incidence of pulmonary autoantibodies. We saw a direct relationship between the titre and the degree of severity of the disease or the clinical condition. The demonstration of circulating pulmonary autoantibodies in bronchial asthma is possibly not only of diagnostic value but also of prognostic importance. PMID:135497

  11. [The effect of diet therapy on the hormonal spectrum of patients with bronchial asthma].

    Science.gov (United States)

    Chernyshova, T E; Vakhrushev, Ia M; Bydanov, V A; Petrova, R I; Morozova, L E

    1990-01-01

    Results are reported of an investigation of the levels of cortisol, pancreatic and thyroid hormones in 38 patients with the infectious-allergic form of bronchial asthma. Unloading dietotherapy was accompanied by an improvement of the clinical course of the disease, reduction of necessity in broncholytic and hormonal agents. During the unloading dietotherapy period some variants of hormonal response to hunger were revealed. Patients with a severe course of the acidotic crisis revealed a reduction of the insulin level, changes in the cortisol dynamics, T4, a tendency to an increase of T3 in the blood. PMID:2330711

  12. Assessment of the adequacy of bronchial stenting by flow-volume loops

    Energy Technology Data Exchange (ETDEWEB)

    McLaren, Clare A.; Roebuck, Derek J. [Great Ormond Street Hospital for Children, Department of Radiology, London (United Kingdom); Pigott, Nick; Elliott, Martin J. [Great Ormond Street Hospital for Children, Cardiothoracic Unit, London (United Kingdom); Dunne, Catherine [Great Ormond Street Hospital for Children, Department of Physiotherapy, London (United Kingdom)

    2006-08-15

    Airway compression is a common problem in children with certain forms of congenital heart disease. Although various surgical approaches are available to overcome this form of airway obstruction, internal stenting is necessary in a minority of patients. It can be difficult to assess the success of stenting at the time of the procedure, and the interval to successful extubation is usually used as an outcome measure. Measurement of relevant parameters of respiratory physiology with flow-volume and volume-pressure loops permits immediate quantitative assessment of the adequacy of stenting. A 3-month-old infant who underwent bronchial stenting and physiological assessment at the time of the procedure is described. (orig.)

  13. Virus Infection-Induced Bronchial Asthma Exacerbation

    Directory of Open Access Journals (Sweden)

    Mutsuo Yamaya

    2012-01-01

    Full Text Available Infection with respiratory viruses, including rhinoviruses, influenza virus, and respiratory syncytial virus, exacerbates asthma, which is associated with processes such as airway inflammation, airway hyperresponsiveness, and mucus hypersecretion. In patients with viral infections and with infection-induced asthma exacerbation, inflammatory mediators and substances, including interleukins (ILs, leukotrienes and histamine, have been identified in the airway secretions, serum, plasma, and urine. Viral infections induce an accumulation of inflammatory cells in the airway mucosa and submucosa, including neutrophils, lymphocytes and eosinophils. Viral infections also enhance the production of inflammatory mediators and substances in airway epithelial cells, mast cells, and other inflammatory cells, such as IL-1, IL-6, IL-8, GM-CSF, RANTES, histamine, and intercellular adhesion molecule-1. Viral infections affect the barrier function of the airway epithelial cells and vascular endothelial cells. Recent reports have demonstrated augmented viral production mediated by an impaired interferon response in the airway epithelial cells of asthma patients. Several drugs used for the treatment of bronchial asthma reduce viral and pro-inflammatory cytokine release from airway epithelial cells infected with viruses. Here, I review the literature on the pathogenesis of the viral infection-induced exacerbation of asthma and on the modulation of viral infection-induced airway inflammation.

  14. [Bronchial rupture in blunt thoracic trauma].

    Science.gov (United States)

    López Espadas, F; Zabalo, M; Encinas, M; Díaz Regañón, G; Pagola, M A; González Fernández, C

    2000-12-01

    In closed chest trauma, bronchial rupture is an unusual but potentially serious complication, with an associated mortality rate of 30%. Recent decades have seen an increase in incidence parallel to greater use of transport. Eighty percent of injuries are located 2.5 cm from the carina. Diagnosis is based on clinical signs, imaging and bronchoscopy. Subcutaneous emphysema and respiratory insufficiency are the most common findings. Images show the presence of pneumothorax, pneumomediastinum or both. Bronchoscopy is the diagnostic method of choice and must be performed early. Treatment consists of reestablishing anatomical continuity of the tracheobronchial tree by surgical repair if the lesion affects more than a third of the circumference and/or pneumothorax is not resolved after two chest drainages. This type of injury should be recognized and treated early, both to restore lung function and to prevent associated complications caused by delay. However, initial findings are seldom specific, requiring the physician to display a high degree of suspicion and explaining why diagnosis often comes late. PMID:11171438

  15. Bronchial Artery Aneurysm Embolization with NBCA

    International Nuclear Information System (INIS)

    We present a case of asymptomatic bronchial artery aneurysm that formed a fistula with part of the pulmonary artery (there was no definite fistula with the pulmonary vein). We were able to catheterize the feeding vessel but could not reach the aneurysm. We therefore injected a mixture of N-butyl-2-cyanoacrylate (NBCA; Histoacryl, B. Braun, Melsungen, Germany) and iodized oil (Lipiodol; Guerbet, Aulnay-sous-Bois, France) from the feeding vessel. The fistula, aneurysm, and feeding vessel were almost totally occluded. After embolization, the patient coughed a little; there were no other definite side effects or complications. One and 3 months later, on chest CT, the aneurysm was almost completely occupied with hyperattenuating NBCA-Lipiodol embolization. NBCA is a liquid embolization material whose time to coagulation after injection can be controlled by diluting it with Lipiodol. It is therefore possible to embolize an aneurysm, feeding vessels, and efferent vessels (in our case, it was a fistula) by using an NBCA-Lipiodol mixture of an appropriate concentration, regardless of whether the catheter can reach the aneurysm or not

  16. Knowledge and Attitudes of GPs in Saxony-Anhalt concerning the Psychological Aspects of Bronchial Asthma: A Questionnaire Study

    OpenAIRE

    Werwick Katrin; Adolf Daniela; Reed Mark G; Herrmann Marcus

    2010-01-01

    Abstract Bronchial Asthma is a worldwide condition with particularly high prevalence in first world countries. The reasons are multifactorial but a neglected area is the psychological domain. It is well known that heavy emotions can trigger attacks and that depression negatively affects treatment outcomes. It is also known that personality type has a greater effect on disease prevalence than in many other conditions. However, many potential psychological treatments are hardly considered, neit...

  17. Differentiation of human bronchial epithelial cells: role of hydrocortisone in development of ion transport pathways involved in mucociliary clearance.

    Science.gov (United States)

    Zaidman, Nathan A; Panoskaltsis-Mortari, Angela; O'Grady, Scott M

    2016-08-01

    Glucocorticoids strongly influence the mucosal-defense functions performed by the bronchial epithelium, and inhaled corticosteroids are critical in the treatment of patients with inflammatory airway diseases such as asthma, chronic obstructive pulmonary disease, and cystic fibrosis. A common pathology associated with these diseases is reduced mucociliary clearance, a defense mechanism involving the coordinated transport of salt, water, and mucus by the bronchial epithelium, ultimately leading to retention of pathogens and particles in the airways and to further disease progression. In the present study we investigated the role of hydrocortisone (HC) in differentiation and development of the ion transport phenotype of normal human bronchial epithelial cells under air-liquid interface conditions. Normal human bronchial epithelial cells differentiated in the absence of HC (HC0) showed significantly less benzamil-sensitive short-circuit current than controls, as well as a reduced response after stimulation with the selective β2-adrenergic receptor agonist salbutamol. Apical membrane localization of epithelial Na(+) channel α-subunits was similarly reduced in HC0 cells compared with controls, supporting a role of HC in the trafficking and density of Na(+) channels in the plasma membrane. Additionally, glucocorticoid exposure during differentiation regulated the transcription of cystic fibrosis transmembrane conductance regulator and β2-adrenergic receptor mRNAs and appeared to be necessary for the expression of cystic fibrosis transmembrane conductance regulator-dependent anion secretion in response to β2-agonists. HC had no significant effect on surface cell differentiation but did modulate the expression of mucin mRNAs. These findings indicate that glucocorticoids support mucosal defense by regulating critical transport pathways essential for effective mucociliary clearance. PMID:27306366

  18. Imaging of bronchial carcinoid tumors associated to Cushing syndrome with 111In-Octreoscan scintigraphy and immunoscintigraphy with anti-chromogranin monoclonal antibodies. Report of two cases.

    Science.gov (United States)

    Carretta, A; Chiesa, G; Magnani, P; Songini, C; Melloni, G; Zannini, P; Grossi, A

    1997-04-01

    Bronchial carcinoid tumors are neuroendocrine neoplasms capable of expressing somatostatin receptors and of secreting neuromediators such as ACTH and chromogranins. Radiologic appearance is usually non-specific and has to be distinguished from benign pulmonary nodules and other malignant diseases. Standard radiological techniques have limited accuracy in the evaluation of such lesions. Radioisotopic imaging techniques may increase the specificity of diagnostic assessment. The role of immunoscintigraphy with anti-chromogranin A and B monoclonal antibodies (MoAbs) and of 111In-Octreoscan scintigraphy is evaluated in two cases of bronchial carcinoid tumors associated to Cushing syndrome. PMID:9201136

  19. Bronchial Anthracotic Change in South Khorasan Province (Iran, Emphasizing its Association with Tuberculosis

    Directory of Open Access Journals (Sweden)

    Sayyed Gholamreza Mortazavi-Moghaddam

    2014-09-01

    Full Text Available Background: There are many reports on the association between anthracosis and tuberculosis. This study focuses on bronchial anthracosis and associated diseases in the province of South Khorasan-Iran. Methods: This case-series study is performed on patients referred to the Vali-e-Asre Hospital (South Khorasan-Iran for bronchoscopic evaluations during the period of 2009-2012. Written informed consents were obtained prior to bronchoscopic evaluations. The criterion for diagnosis of bronchial anthracosis was black pigmentation on direct observation of bronchus. Bronchial anthracosis was classified into simple (without deformity or complicated (with deformity. Pulmonary tuberculosis (TB was diagnosed either by acid fast staining and culture of liquid samples, or histopathology examination of biopsy. Spirometry was performed to determine the obstructive or restrictive pattern. Results: Among 279 patients who underwent bronchoscopic evaluations, 89 patients, including 34 males (38.2% and 55 (61.79% females, were diagnosed with anthracosis. Simple and complicated anthracosis were observed in 42 (48.2% and 47 (52.8% cases respectively. Mean age of patients was 72.23±9.65 years. There were 43 (48.3% cases of tuberculosis (28 cases with complicated and 15 cases with simple anthracosis (P=0.021. Chest X-ray showed consolidation/infiltration, reticular/fibrotic, and mass/nodule/hilar prominence in 57 (64%, 26 (29.21% and 6 (6.74% cases, respectively. Bronchitis was reported in 42 (%59.15 out of 79 patients whose biopsy samples were taken. Spirometric patterns were obstructive, restrictive, upper airway obstruction, and normal in 45 (50.56%, 32 (35.95%, 2 (2.24%, and 10 (11.23% patients respectively. Conclusion: Tuberculosis is the most frequent disease associated with anthracosis in South Khorasan province. Consequently, patients with anthracosis must be carefully evaluated for tuberculosis.

  20. Recurrent pneumothorax associated with bronchial atresia: report of a case.

    Science.gov (United States)

    Tanaka, Kazuhisa; Suzuki, Hidemi; Nakajima, Takahiro; Tagawa, Tetsuzo; Iwata, Takekazu; Mizobuchi, Teruaki; Yoshida, Shigetoshi; Yoshino, Ichiro

    2015-10-01

    We herein report a case of recurrent pneumothorax associated with congenital bronchial atresia. A 26-year-old male presented with chest pain. Chest roentgenograms showed left pneumothorax, a left apical bulla and an area of hyperlucency in the left upper lung field, and chest computed tomography revealed a discontinuation of the left superior bronchus. Additionally, both ventilation and perfusion scintigraphy showed a defect in the left superior segment. A thoracoscopy-assisted left superior segmentectomy was performed, and a pathological examination indicated left superior segmental bronchial atresia, which might have predisposed the peripheral lung to emphysematous conditions. No relapse was observed 6 months after the operation. Although this entity is rare, congenital bronchial atresia should be considered in the differential diagnosis when a patient has suffered from a recurrent spontaneous pneumothorax.

  1. Photodynamic therapy (PDT) in advanced inoperable bronchial carcinoma

    Science.gov (United States)

    Moghissi, Keyvan; Dixon, Kate; Stringer, Mark R.; Brown, Stanley B.

    1996-12-01

    Objective: To assess the efficacy of PDT to: Palliate symptoms, control disease and extend survival in patients with advanced inoperable cancer. Subject and Method: 55 Males and 23 females aged between 45-81 years (mean 66 years) with inoperable and advanced lung cancer with > 5O. obstructive lesions of the main, lobar or segmental bronchi. Patients had pre-treatment routine clinical radiological, functional and endoscopic assessment with proven histological diagnosis. Protocol of PDT was; Intravenous injection of 2 mg/Kg bodyweight Polyhaematoporphyrin (equivalent to Photofrin) or Photofrin followed 24-72 hours later by illumination of tumour using 630 nm light (Oxford Laser) delivered via an optical fibre with end diffuser. Treatments were carried out under general anaesthesia as a day case procedure. Patients were rebronchoscoped for debridement/retreatment 4-7 days later. Results: There was no treatment related mortality. Two patients developed mild photosensitivity reaction. All patients showed symptomatic improvement with good initial functional and radiological amelioration. Every patient responded to treatment. Seven patients had complete response and negative histology for 3-12 months. After the first treatment average Forced Vital Capacity (FVC) and Forced Expiratory Volume in one second (FEV1) improvement was 0.5 litres and 0.4 litres respectively. Twenty five percent of patients (nr 19) survived more than 2 years, 10'. (nr=8) between 1-2 years and the remaining 51 patients less than a year. Conclusion: PDT should be considered as a therapeutic modality for all stages of lung cancer and is an excellent treatment modality for palliation in advanced bronchial malignancies.

  2. Bronchial Sparganosis mansoni accompanied by abnormal hyperplasia diagnosed by bronchoscopy

    Institute of Scientific and Technical Information of China (English)

    BAI Jing; HE Zhi-yi; LIU Guang-nan; ZHANG Jian-quan; DENG Jing-min; LI Mei-hua; ZHONG Xiao-ning

    2012-01-01

    Pulmonary sparganosis mansoni is rare in humans and bronchial sparganosis mansoni has not been reported.We reported a patient with a soft-tissue mass in the right hilum area on a chest computed tomography (CT) scan that was suspected of being lung cancer.Bronchoscopy identified sparganum larvae.Bronchial sparganosis mansoni accompanied by abnormal hyperplasia was diagnosed by histopathology.We introduced our experience and reviewed the clinical characteristics of three pulmonary sparganosis mansoni cases and three pleural cavity sparganosis mansoni cases that have been reoorted.

  3. Lithium Attenuates TGF-β1-Induced Fibroblasts to Myofibroblasts Transition in Bronchial Fibroblasts Derived from Asthmatic Patients

    Directory of Open Access Journals (Sweden)

    Marta Michalik

    2012-01-01

    Full Text Available Bronchial asthma is a chronic disorder accompanied by phenotypic transitions of bronchial epithelial cells, smooth muscle cells, and fibroblasts. Human bronchial fibroblasts (HBFs derived from patients with diagnosed asthma display predestination towards TGF-β-induced phenotypic switches. Since the interference between TGF-β and GSK-3β signaling contributes to pathophysiology of chronic lung diseases, we investigated the effect of lithium, a nonspecific GSK-3β inhibitor, on TGF-β1-induced fibroblast to myofibroblast transition (FMT in HBF and found that the inhibition of GSK-3β attenuates TGF-β1-induced FMT in HBF populations derived from asthmatic but not healthy donors. Cytoplasmically sequestrated β-catenin, abundant in TGF-β1/LiCl-stimulated asthmatic HBFs, most likely interacts with and inhibits the nuclear accumulation and signal transduction of Smad proteins. These data indicate that the specific cellular context determines FMT-related responses of HBFs to factors interfering with the TGF-β signaling pathway. They may also provide a mechanistic explanation for epidemiological data revealing coincidental remission of asthmatic syndromes and their recurrence upon the discontinuation of lithium therapy in certain psychiatric diseases.

  4. RELATIONSHIP BETWEEN PSYCHOPATHOLOGY & SOCIO-DEMOGRAPHIC AND CLINICAL VARIABLES IN COPD AND BRONCHIAL ASTHMA: A COMPARATIVE STUDY

    Directory of Open Access Journals (Sweden)

    Mayank

    2014-11-01

    Full Text Available This is a cross sectional comparative case control study assessing the Relationship between psychopathology and socio demographic and clinical variables in chronic obstructive pulmonary disease (COPD and Bronchial Asthma in comparison to healthy individuals. The investigation reveals that there is psychopathology in all the three groups. The psychopathology is significantly more in patients with COPD. Psychopathology in COPD patients is related to age, duration, severity of illness and steroid medication. It is found that psychopathology in bronchial asthma patients are more than those of healthy controls but there is no significant difference. The psychopathology is significantly related to relate to age, marital status, occupational status, and smoking. Duration severity of illness and steroid medication.

  5. 11.3.Upper respiratory tract disease and bronchial asthma

    Institute of Scientific and Technical Information of China (English)

    1993-01-01

    930295 Factors influencing carboxyhemog-lobin kinetics in inhalation lung injury.WU Wenxi (吴文溪).ist Affili Hosp,Nanjing MedCoil,210029.Chin J Intern Med 1992;31 (11):689—691.Anesthetized dogs were ventilated with 1%carbon monoxide (CO) in air for 10 minutes toproduce CO poisoning and then with room air (n=5) or pure oxygen (n=5) for 3 hours as con-trol.Acute lung injury was produced by intratra-cheal injection of 0.1 N HC1 (2 ml/kg) 30 min-utes before CO poisoning in another 10 experi-mental dogs.Arterial blood gas and earboxyhe-moglobin (COHb) were monitored before andafter CO poisoning.Pharmacokinetic analysis

  6. 11.3.Upper respiratory tract disease and bronchial asthma

    Institute of Scientific and Technical Information of China (English)

    1992-01-01

    920096 A clinical trial of treating asthma ofmoderate severity with beclomethason dip-ropionate aerosol.SHEN Xun (沈勋),et al.DeptPulmon Med,Zhongshan Hosp,Shanghai MedUniv,200032.Chin J Intern Med 1991; 30 (9):536-538.In order to investigate the efficacy of steroidinhalation in treating asthma of moderate sever-

  7. Smoking-induced gene expression changes in the bronchial airway are reflected in nasal and buccal epithelium

    Directory of Open Access Journals (Sweden)

    Zhang Xiaohui

    2008-05-01

    Full Text Available Abstract Background Cigarette smoking is a leading cause of preventable death and a significant cause of lung cancer and chronic obstructive pulmonary disease. Prior studies have demonstrated that smoking creates a field of molecular injury throughout the airway epithelium exposed to cigarette smoke. We have previously characterized gene expression in the bronchial epithelium of never smokers and identified the gene expression changes that occur in the mainstem bronchus in response to smoking. In this study, we explored relationships in whole-genome gene expression between extrathorcic (buccal and nasal and intrathoracic (bronchial epithelium in healthy current and never smokers. Results Using genes that have been previously defined as being expressed in the bronchial airway of never smokers (the "normal airway transcriptome", we found that bronchial and nasal epithelium from non-smokers were most similar in gene expression when compared to other epithelial and nonepithelial tissues, with several antioxidant, detoxification, and structural genes being highly expressed in both the bronchus and nose. Principle component analysis of previously defined smoking-induced genes from the bronchus suggested that smoking had a similar effect on gene expression in nasal epithelium. Gene set enrichment analysis demonstrated that this set of genes was also highly enriched among the genes most altered by smoking in both nasal and buccal epithelial samples. The expression of several detoxification genes was commonly altered by smoking in all three respiratory epithelial tissues, suggesting a common airway-wide response to tobacco exposure. Conclusion Our findings support a relationship between gene expression in extra- and intrathoracic airway epithelial cells and extend the concept of a smoking-induced field of injury to epithelial cells that line the mouth and nose. This relationship could potentially be utilized to develop a non-invasive biomarker for

  8. Allergic disease as an association of steroid sulphatase deficiency.

    Science.gov (United States)

    Sakura, N; Nishimura, S; Matsumoto, T; Ohsaki, M; Ogata, T

    1997-11-01

    Ten of 31 patients with steroid sulphatase (STS) deficiency were found to have an allergic disease (bronchial asthma, allergic rhinitis, or atopic dermatitis). STS deficiency may predispose patients to allergic disease.

  9. Comparison of bronchial brushing and sputum in detection of pediatric pulmonary tuberculosis.

    Science.gov (United States)

    Chen, Qiao-Pei; Ren, Shi-Feng; Wang, Xin-Feng; Wang, Mao-Shui

    2016-01-27

    The retrospective study aimed to evaluate the diagnostic value of bronchial brushing and sputum using acid fast bacilli smear, mycobacterial culture and real-time PCR in detection of pediatric pulmonary tuberculosis, sensitivity and specificity of bronchial brushing and sputum examined by the three methods were calculated and compared to each other. Data showed there were no significant difference in sensitivity between bronchial brushing and matched sputum using each method. But the specificity of real-time PCR on bronchial brushing was lower than on sputum. Compared with bronchial brushing, sputum was better specimen in detection of pediatric pulmonary tuberculosis.

  10. Airway Inflammation and Bronchial Hyperresponsiveness in Elite Cross-Country Skiers and in Patients with Newly Diagnosed Asthma: A Bronchial Biopsy Study

    OpenAIRE

    Karjalainen, Eeva-Maija

    2008-01-01

    The objective of these studies was to evaluate possible airway inflammation and remodeling at the bronchial level in cross-country skiers without a prior diagnosis of asthma, and relate the findings to patients with mild chronic asthma and patients with newly diagnosed asthma. We also studied the association of airway inflammatory changes and bronchial hyperresponsivess (BHR), and treatment effects in cross-country skiers and in patients with newly diagnosed asthma. Bronchial biopsies we...

  11. PSYCHIATRIC MORBIDITIES IN PATIENTS OF COPD AND BRONCHIAL ASTHMA ATTENDING OPD OF TERTIARY CARE HOSPITAL

    Directory of Open Access Journals (Sweden)

    Ab. Majid

    2016-03-01

    Full Text Available BACKGROUND Chronic pulmonary diseases are a global health problem and the number of patients being treated in primary care settings is increasing. The prevalence of a life-time diagnosis of asthma has increased in all age groups. There has been a dramatic shift in the conceptualization and treatment of asthma and COPD in the last 50 years. Psychiatric disorders, especially anxiety disorders, are very common in patients with asthma and COPD. Anxiety is also common in COPD and is related to some of the same factors described for asthma including the psychological response to the experience of breathlessness as well as side effects of beta-agonists. Since there is paucity of relevant data from Kashmir, this study was designed to find the co morbidity in patients of COPD and Asthma in a tertiary care teaching hospital in Srinagar, Kashmir. METHODS Sixty successive patients presents with history of thirty each of asthma and COPD who fulfilled inclusion and exclusion criteria were taken up for the study and administered the Hamilton depression rating scale (HAM-D and Hamilton Anxiety rating scale (HAM-A scales for evaluation of depressive disorder and anxiety disorder. Each patient was informed about the purpose of interview; his/her consent was obtained and strict confidentiality was ensured. General description, demographic data and psychiatric history were recorded using semi structured Proforma and HAM-A and HAM-D. RESULTS Out of sixty cases of COPD and Bronchial Asthma, 50% of the patients were in the age group of 66-80 followed by 35% in age group of 81-85 years. This could suggest that COPD and Bronchial Asthma is a problem of old age. There were predominantly more males (65% than females (35% in our study. Representations of gender, religion, family type and marital status have been found to be in accordance with socio-demographic profile of our country. Out of sixty cases of COPD and Bronchial Asthma, 35% of the patients were educated up to

  12. Study of Therapeutic Mechanism of Acupuncture in Treating Bronchial Asthma

    Institute of Scientific and Technical Information of China (English)

    2001-01-01

    @@  It has been considered by modern medicine that bronchial asthma is a chronic airway allergic inflammation (AAI) which is the major factor that induces reversible airway ventilating disturbance and bronchial hyper-responsiveness (BHR) in asthmatic patients, as well as a correlation between the delayed-phase onset of asthma and AAI is more significant than immediate-phase onset of asthma in its pathogenesis. Anti-inflammatory therapy as a fundamental principle of treatment for bronchial asthma has been brought forward during the interval phase so that a large number of anti-AAI drugs to significantly enhance the curative effect such as inhalant corticoid, disodium cromoglycate, and leukotriene receptors antagonist, etc., has been rapidly developed. Additionally, traditional Chinese medicine (TCM) and integration of TCM and western medicine (WM) therapies, including acupuncture, are gradually taken into account by the medical circle as a characteristic in our country for treatment of bronchial asthma. It is worthy of being pointed out that the curative effect of acupuncture and moxibustion in treating asthma is evident, and that its therapeutic mechanism has to a certain extent, been found along with the recent deep-going acupunctural research.

  13. Study of Therapeutic Mechanism of Acupuncture in Treating Bronchial Asthma

    Institute of Scientific and Technical Information of China (English)

    LI; Ji

    2001-01-01

    It has been considered by modern medicine that bronchial asthma is a chronic airway allergic inflammation (AAI) which is the major factor that induces reversible airway ventilating disturbance and bronchial hyper-responsiveness (BHR) in asthmatic patients, as well as a correlation between the delayed-phase onset of asthma and AAI is more significant than immediate-phase onset of asthma in its pathogenesis. Anti-inflammatory therapy as a fundamental principle of treatment for bronchial asthma has been brought forward during the interval phase so that a large number of anti-AAI drugs to significantly enhance the curative effect such as inhalant corticoid, disodium cromoglycate, and leukotriene receptors antagonist, etc., has been rapidly developed. Additionally, traditional Chinese medicine (TCM) and integration of TCM and western medicine (WM) therapies, including acupuncture, are gradually taken into account by the medical circle as a characteristic in our country for treatment of bronchial asthma. It is worthy of being pointed out that the curative effect of acupuncture and moxibustion in treating asthma is evident, and that its therapeutic mechanism has to a certain extent, been found along with the recent deep-going acupunctural research.……

  14. Lung function, bronchial hyperresponsiveness, and atopy among firefighters

    NARCIS (Netherlands)

    Greven, Frans; Krop, Esmeralda; Spithoven, Jack; Rooyackers, Jos; Kerstjens, Huib; Heederik, Dick

    2011-01-01

    Objectives The aim of this study was to determine associations between lung function, bronchial hyperresponsiveness (BHR), and atopy with exposure to fire smoke among firefighters. Methods The study was comprised of 402 firefighters, a randomly chosen subset of a previous survey among firefighters i

  15. Morgagni’s hernia in a woman with bronchial asthma

    Directory of Open Access Journals (Sweden)

    Gowrinath K

    2014-07-01

    Full Text Available A 59-year-old, other wise asymptomatic lady known to have bronchial asthma was found to have an anterior mediastinal mass on a chest radiograph incidentally obtained as a part of pre-operative work-up for cataract surgery. Computed tomography of the chest confirmed the diagnosis of Morgagni’s hernia.

  16. Dry powder formulation in the twincertm for bronchial challenge testing

    NARCIS (Netherlands)

    Lexmond, A.J.; Hagedoorn, P.; Frijlink, H.W.; Ten Hacken, N.H.T.; Steckel, H.; De Boer, A.H.

    2013-01-01

    Summary Background: In bronchial challenge testing lung deposition of the stimulus may be poorly controlled due to incorrect use of nebulisers. Furthermore, the need for freshly prepared solutions burdens personnel and budget. In this study we aim to develop a dry powder alternative with higher repr

  17. Mechanical compression attenuates normal human bronchial epithelial wound healing

    Directory of Open Access Journals (Sweden)

    Malavia Nikita

    2009-02-01

    Full Text Available Abstract Background Airway narrowing associated with chronic asthma results in the transmission of injurious compressive forces to the bronchial epithelium and promotes the release of pro-inflammatory mediators and the denudation of the bronchial epithelium. While the individual effects of compression or denudation are well characterized, there is no data to elucidate how these cells respond to the application of mechanical compression in the presence of a compromised epithelial layer. Methods Accordingly, differentiated normal human bronchial epithelial cells were exposed to one of four conditions: 1 unperturbed control cells, 2 single scrape wound only, 3 static compression (6 hours of 30 cmH2O, and 4 6 hours of static compression after a scrape wound. Following treatment, wound closure rate was recorded, media was assayed for mediator content and the cytoskeletal network was fluorescently labeled. Results We found that mechanical compression and scrape injury increase TGF-β2 and endothelin-1 secretion, while EGF content in the media is attenuated with both injury modes. The application of compression after a pre-existing scrape wound augmented these observations, and also decreased PGE2 media content. Compression stimulated depolymerization of the actin cytoskeleton and significantly attenuated wound healing. Closure rate was partially restored with the addition of exogenous PGE2, but not EGF. Conclusion Our results suggest that mechanical compression reduces the capacity of the bronchial epithelium to close wounds, and is, in part, mediated by PGE2 and a compromised cytoskeleton.

  18. Lipocalin2 protects against airway inflammation and hyperresponsiveness in a murine model of allergic airway disease

    DEFF Research Database (Denmark)

    Dittrich, A M; Krokowski, M; Meyer, H-A;

    2010-01-01

    Allergen-induced bronchial asthma is a chronic airway disease that involves the interplay of various genes with environmental factors triggering different inflammatory pathways.......Allergen-induced bronchial asthma is a chronic airway disease that involves the interplay of various genes with environmental factors triggering different inflammatory pathways....

  19. [Right tracheal bronchus with anomalous ramification of the bronchial artery disclosed during an episode of hemoptysis].

    Science.gov (United States)

    Kyo, S; Maeda, H; Yahata, T; Kawashima, T; Takada, T; Ohnishi, K; Adachi, K

    2000-01-01

    A 63-year-old woman was referred to our hospital on June 18th, 1998 during an episode of hemoptysis that had lasted for 6 days. She had no hemorrhagic diathesis and no history of pulmonary disease. Chest X-ray films disclosed a ground-glass opacity in the right upper lung field. Bronchoscopic examination revealed bleeding from an anomalous ectopic orifice on the right lateral trachea, about 1 cm above the carina. Chest computed tomographic examinations by conventional and spiral methods readily disclosed an ectopic bronchus. Bronchial arteriography showed that the tracheal bronchus was fed by a branched vessel of the thyrocervical artery arising from the brachiocephalic artery. Atypical mycobacterium was detected in bronchoalveolar lavage fluid from the ectopic bronchus. A shunt had formed with the pulmonary artery and peripheral parts of the bronchial artery that fed the tracheal bronchus. It was speculated that the hemoptysis in this case might be due to the combined phenomena of infection and abnormal vessel formation in the tracheal bronchus. In our patient, the system of blood supply to the tracheal bronchus may have been a manifestation of atavism because it closely resembled the circulatory structure of the tracheal bronchi normally observed in sheep and giraffes. The tracheal bronchus should be taken into consideration as a potential cause of hemoptysis, inflammatory changes, and atelectasis during intubation. PMID:10723948

  20. [Macro-and microelements imbalance in etiology and progression of bronchial asthma in children].

    Science.gov (United States)

    Lytvynets', L Ia

    2013-06-01

    The aim of our work was the study of macro-and microelemensts characteristics of the state of the blood serum of children with asthma, depending on the severity. A total of 107 children aged 10 to 18 years with asthma in the acute stage. The results of the proposed asthma control test (GINA, 2011) to the level of asthma control the children were distributed as follows: 34 (31.8%)--with controlled, 47 (43.9%)--partly controlled and 26--with uncontrolled bronchial asthma. Investigation of serum trace elements were determined by atomic absobtsionnoy spectrophotometry. Found that with varying degrees of control of asthma changes were reduction of magnesium, zinc, when excessive amounts of copper and calcium. We show some dizelements in patients with nontrol bronhial asthma. The most pronounced manifestation dyzelements documented in patients with uncontrolled asthma. It is with same with uncontrolled asthma, there are marked hypoxic change, energy shortage, bronchial hyperreactivity, as a result of increasing stress level of response to an antigen, reduce antioxidant enzyme systems, immune system dysfunction. This changes in the macro-and microelement composition of the blood significantly reduce the reserves of the immune system, chronic inflammatory processes in the bronches, which exacerbates the severity of the disease, which in practice requires a differential diagnosis, comprehensive treatment based on identified dyzelements states.

  1. Uptake of 12-HETE by human bronchial epithelial cells (HBEC): effects on HBEC cytokine production.

    Science.gov (United States)

    Gormand, F; Chabannes, B; Moliere, P; Perrin-Fayolle, M; Lagarde, M; Pacheco, Y

    1996-04-01

    12-HETE, the major lipoxygenase end-product of platelets and macrophages, may be released in contact of bronchial epithelium in inflammatory diseases of the lung. We have studied the outcome of 12-HETE in presence of human bronchial epithelial cells (HBEC). When HBEC were incubated with [3H]12-HETE for 30 minutes, 27.5% of total radioactivity was found in HBEC and 72.5% in supernatants. Unesterified 12-HETE accounted for 22.4% of total radioactivity, 4.5% being recovered in phospholipids, preferentially in phosphatidylcholine and phosphatidylethanolamine. No incorporation in neutral lipids was detected. 72.9% of the incubated radioactivity was recovered in un identified metabolites. As 12-HETE has been shown to modulate the expression and production of various proteins, the consequence of the 12-HETE uptake on the release of GM-CSF and IL8 by HBEC was assessed. HBEC from control subjects were cultured for 24 hours with 12-HETE (10(-9) to 10(-7)M) in the presence or absence of TNF alpha. Detectable amounts of both cytokines were released in the supernatant in basal conditions at 24hr, and TNF alpha increased significantly the release of GM-CSF. 12-HETE at 10(-7)M weakly but significantly decreased the TNF-induced release of GM-CSF from HBEC. Thus the uptake of 12-HETE could affect the epithelial cell function in some situations.

  2. The usage of sustained-action theophyline in the intercrisis treatment of bronchial asthma.

    Directory of Open Access Journals (Sweden)

    Yaquelín Martínez Chavez.

    2004-04-01

    Full Text Available Fundaments: Bronchial Asthma is a chronic inflammatory disease of the air ways. Theophylline is recommended in its treatment because of its probable anti-inflamatory effect. Due to this reason, the clinical effect of sustained action theophylline (TEOCEN 200mg is assessed in the intercrisis of Bronchial Asthma. Method : 40 moderate and severe asthmatic patients received treatment with a 9 mg/kg/day dose every 12 hours (q.12, at the outpatient consultation of the University Hospital Celestino Hdez¨ of Villa Clara, from September 2002 to June 2003. Treatment lasted a month, period in which had 5 visits to the doctor. The variables under study in each visit were: attendance to the emergency department, use of salbutamol spray and objective measurement of lung function as well as adverse effects and response to treament. Wilconxon, Cochron Q and Mc. Nemor´s non-parametric tests were used in this study. Significant difference was considered as p< 0, 05, highly significant difference as p< 0,01. Results : The use of salbutamol spray and the need to go to the emergency department diminished significantly meanwhile the peak expiratory flux, the forced expiratory volume in one second, the maximum expiratory medium flux and the forced expiratory volume in one second post salbutamol application increased significantly. There was a low incidence of adverse effects. Conclusion : The formula was useful and is recommended in the control of the symptoms of moderate and severe asthmatic patients in the intercrisis period.

  3. Prevalence and risk factors for bronchial asthma in adults in Jaipur district of Rajasthan (India

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    Gupta P

    2006-01-01

    Full Text Available No information is available on epidemiology of bronchial asthma in Rajasthan. A field study was therefore, undertaken to estimate prevalence of bronchial asthma and to define the risk factors influencing the disease prevalence in Jaipur district of the state using a previously validated questionnaire. Besides demographic data, information on smoking habits, domestic cooking fuel used, atopic symptoms, and family history suggestive of asthma was also collected. Multivariate logistic regression analysis was performed to calculate odds ratio of various potential risk factors. Data from 8863 respondents (5010 men, 3853 women were analyzed after excluding children below 15 years of age. One or more respiratory symptoms were present in 5.3% of the subjects. Asthma was diagnosed in 1.86%, 1.44%, 0.51 and 0.38% of the rural male, rural female, urban male and urban female respondents respectively, with an overall prevalence of 0.96%. History of atopy in self and/or history of atopy/asthma in the first-degree relative were found to be the most important risk factor. Advancing age, usual residence in rural area and tobacco smoking, especially hookah smoking, were also associated with significantly higher odds of having asthma.

  4. Chemiluminescence of neutrophiles stimulated by opsonized Zymosan in children with bronchial asthma and pneumonia

    Science.gov (United States)

    Lewandowicz-Uszynska, A.; Jankowski, A.

    2004-08-01

    Oxygen metabolism of neutrophils after stimulation with opsonized zymosan was examined using chemiluminescence test (in the presence of the patient serum or pooled serum). Into the study 37 children aged from 2 to 12 years were enrolled (20 girls and 17 boys). 10 healthy volunteers comprised the control group (group III). Two groups of patients were established: group I -- children with bronchial asthma (without infection), group II -- children with pneumonia. The examination in both groups was performed twice -- in acute phase and in remission period. The group I in acute phase comprised 16 children and in remission phase 9 children, group II - 21 children in acute phase and 9 children in remission phase, respectively. The following parameters of CL were estimated average value of so called spontaneous CL, maximal excitation of neutrophils after stimulation by zymogen (CLmax), time of zymosan opsonization. The following results were obtained: increased spontaneous CL and CLmax (at the presence of both sera) in acute phase of bronchial asthma and pneumonia in comparison to the control group. In the period of remission both these parameters were insignificantly decreased. The longest time of zymosan opsonization in acute period of disease was observed in children with pneumonia (18 min.). This time did not change during remission phase. Only slightly longer time of opsonization was observed in the patients from group I (in exacerbation) (15 min) than in the control group (13,1 min). This time was prolonged in the clinical remission (20 min).

  5. The Expression of microRNA-1 and microRNA-126 in Peripheral Blood of Asthmatic Children with Acute Episode of Bronchial Asthma and its Correlation with the Onset of the Disease%microRNA-1与microRNA-126在支气管哮喘急性发作期患儿外周血中的表达及诊断价值

    Institute of Scientific and Technical Information of China (English)

    颜世军; 潘秋莎; 周炳文; 孙雨

    2016-01-01

    目的::探讨microRNA-1与microRNA-126在支气管哮喘急性发作期患儿外周血中的表达及其与患儿发病的相关性。方法:临床纳入2013年1月至2015年1月我院收治的支气管哮喘急性发作期患儿48例,设为哮喘组,同时纳入同时期内52例健康体检儿童作为对照组。分别采集两组外周血,并进行microRNA-1与microRNA-126水平检测,同时检测IL-4、INF-γ水平。结果:哮喘组患儿IL-4、INF-γ水平分别为(109.65±74.31)ng/L、(70.47±11.96)ng/L,对照组患儿IL-4、INF-γ水平分别为(78.47±75.78)ng/L、(77.05±17.21)ng/L,差异有显著性(P<0.05);哮喘组患儿microRNA-1与microR-NA-126水平分别为(2.15±0.97)、(7.34±1.26),对照组患儿microRNA-1与microRNA-126水平分别为(5.81±1.29)、(3.66±0.91),差异有显著性(P<0.05);microRNA-126、microRNA-1在支气管哮喘急性发作期患儿外周血中的灵敏度与在对照组的特异度分别为85.42%、79.17%、78.85%、73.08%;ROC曲线下面积分别为0.917、0.865。结论:支气管哮喘急性发作期患儿外周血中 microRNA-126水平升高, mi-croRNA-1水平降低,两者可以作为临床诊断儿童支气管哮喘急性发作的客观指标。%Objective:To investigate the expression of microRNA-1 and microRNA-126 in peripheral blood in children with acute episode of bronchial asthma and its correlation with the onset of the disease. Methods:48 patients with acute exacerbation of bronchial asthma in our hospital from Jan. 2013 to Jan. 2015 were included in the asthma group, while the control group was included 52 cases of healthy children. The pe-ripheral blood of the two groups were collected, and the level of microRNA-126 and microRNA-1 was detec-ted, and the levels of IL-4 and INF-γwere detected as the same time. Results:The levels of IL-4,INF-γin asthma group were (109.65±74.31)ng/L and (70.47±11.96)ng/L, while those in control group were (78.47 ±75

  6. The effect of various breathing exercises (pranayama in patients with bronchial asthma of mild to moderate severity

    Directory of Open Access Journals (Sweden)

    Saxena Tarun

    2009-01-01

    Full Text Available Background/Aim: The incidence of bronchial asthma is on increase. Chemotherapy is helpful during early course of the disease, but later on morbidity and mortality increases. The efficacy of yoga therapy though appreciated is yet to be defined and modified. Aim: To study the effect of breathing exercises ( pranayama in patients with bronchial asthma of mild to moderate severity. Materials and Methods: Fifty cases of bronchial asthma (Forced Expiratory Volume in one second (FEV1 > 70% were studied for 12 weeks. Patients were allocated to two groups: group A and group B (control group. Patients in group A were treated with breathing exercises (deep breathing, Brahmari , and Omkara , etc. for 20 minutes twice daily for a period of 12 weeks. Patients were trained to perform Omkara at high pitch (forceful with prolonged exhalation as compared to normal Omkara . Group B was treated with meditation for 20 minutes twice daily for a period of 12 weeks. Subjective assessment, FEV1%, and Peak Expiratory Flow Rate (PEFR were done in each case initially and after 12 weeks. Results: After 12 weeks, group A subjects had significant improvement in symptoms, FEV1, and PEFR as compared to group B subjects. Conclusion: Breathing exercises ( pranayama , mainly expiratory exercises, improved lung function subjectively and objectively and should be regular part of therapy.

  7. Expression of polycomb protein BMI-1 maintains the plasticity of basal bronchial epithelial cells.

    Science.gov (United States)

    Torr, Elizabeth; Heath, Meg; Mee, Maureen; Shaw, Dominick; Sharp, Tyson V; Sayers, Ian

    2016-08-01

    The airway epithelium is altered in respiratory disease and is thought to contribute to disease etiology. A caveat to disease research is that the technique of isolation of bronchial epithelial cells from patients is invasive and cells have a limited lifespan. The aim of this study was to extensively characterize the plasticity of primary human bronchial epithelial cells that have been engineered to delay cell senescence including the ability of these cells to differentiate. Cells were engineered to express BMI-1 or hTERT using viral vector systems. Cells were characterized at passage (p) early (p5), mid (p10), and late (p15) stage for: BMI-1, p16, and CK14 protein expression, viability and the ability to differentiate at air-liquid interface (ALI), using a range of techniques including immunohistochemistry (IHC), immunofluorescence (IF), transepithelial electrical resistance (TEER), scanning electron microscopy (SEM), MUC5AC and beta tubulin (BTUB) staining. BMI-1-expressing cells maintained elevated levels of the BMI-1 protein and the epithelial marker CK14 and showed a suppression of p16. BMI-1-expressing cells had a viability advantage, differentiated at ALI, and had a normal karyotype. In contrast, hTERT-expressing cells had a reduced viability, showed limited differentiation, and had an abnormal karyotype. We therefore provide extensive characterization of the plasticity of BMI-1 expressing cells in the context of the ALI model. These cells retain properties of wild-type cells and may be useful to characterize respiratory disease mechanisms in vitro over sustained periods. PMID:27558999

  8. Role of serum eosinophil cationic protein as a biological marker to assess the severity of bronchial asthma

    International Nuclear Information System (INIS)

    Objective: The study was carried out to evaluate the role of serum eosinophil cationic protein (ECP) as a biological marker for the diagnosis and to assess the severity of bronchial asthma. Methodology: This observational cross-sectional study was conducted among 70 bronchial asthma patients and 45 disease controls (tuberculosis-15, chronic obstructive pulmonary disease-15, interstitial lung disease-15) enrolled from patients attending the outpatient department of the National Institute of Disease of the Chest and Hospital (NIDCH), Dhaka, Bangladesh during July 2010 to June 2011. Global Initiative of Asthma Management and Prevention (GINA) criteria were followed for selection of both atopic and non-atopic patients with intermittent or persistent (mild, moderate and severe) asthma. Serum level of eosinophil cationic protein (ECP), IgE, forced expiratory volume in 1 second (FEV 1% predicted) and circulatory eosinophil (CE) count were estimated. Results: Mean serum ECP level (28.8 +- 42.9 vs. 6.82 +- 3.5 ng/mL; P<0.001), IgE level (383.59 - 225.3 vs. 135 +- 131.8 IU/mL; P<0.001) and percent circulatory eosinophil count (9.95 +- 3.7 vs. 5.95 +- 1.4; P<0.024) were all found significantly raised among asthma patients than disease controls but % FEV1 was equivocal. All grades of persistent asthma patients had significantly (P<0.025 and P<0.002) higher mean ECP level than intermittent cases but serum IgE level and CE count did not differ significantly. FEV1 % predicted correlated well among moderate and severe persistent asthma but was equivocal for intermittent and mild persistent cases. Conclusion: This study has reinforced that serum eosinophil cationic protein is a dependable biological marker with more discriminatory power over other indicators for bronchial asthma and to assess its severity. (author)

  9. Respiratory function and bronchial responsiveness among industrial workers exposed to different classes of occupational agents: a study from Algeria

    Directory of Open Access Journals (Sweden)

    Hoet Peter H

    2007-10-01

    Full Text Available Abstract Occupational exposures play a role in the onset of several chronic airway diseases. We investigated, in a cross-sectional study, lung function parameters and bronchial hyper-responsiveness to histamine in workers exposed to different airborne compounds. The study group totalled 546 male subjects of whom 114 were exposed to welding fumes, 106 to solvents, 107 to mineral dust, 97 to organic dust and 123 without known exposure to airway irritants. A questionnaire was administered and spirometry and bronchial responsiveness to histamine were assessed by one observer, in the morning before work to prevent effects of acute exposure. The mean (SD age of the participants was 39.3 (7.8 years, with a mean duration of employment of 13.8 (6.6 years. Both before and after adjustment for smoking status, forced expiratory volume in 1 second (FEV1, expressed as % predicted was lower in welders -4.0% (95% confidence interval [CI], -6.3 to -1.8; p = 0.01 and workers exposed to solvents -5.6% (CI: -7.9 to -3.3; p = 0.0009 than in control subjects. Furthermore, solvent workers had an odds ratio of 3.43 (95% CI: 1.09–11.6; p = 0.037 for bronchial hyperresponsiveness compared with the reference group. The higher prevalence of bronchial hyperresponsiveness in solvent workers adds to the growing body of evidence of adverse respiratory effects of occupational solvent exposure. These results point to the necessity of preventive measures in solvent workers to avoid these adverse respiratory effects.

  10. Sixty cases of bronchial asthma treated with electroacupuncture and cupping%电针配合拔罐治疗支气管哮喘60例

    Institute of Scientific and Technical Information of China (English)

    胡善家

    2011-01-01

    Bronchial asthma is a kind of common chronic intractable disease of repeated attacks.In recent years,the morbidity and mortality of asthma are increasing in the world.At present,about 150 to 200 million people suffer from this disease globally and about 180 000 people are died of asthma annually.Hence,the prevention and treatment of asthma have drawn the global attentions[1].From 2008 to 2010,the writer was under the mission of China's foreign medical aid in Vanuatus,one of the South Pacific Island Nations.Based on the theory of "the medical measurefor the prevention of disease" in Chinese medicine,acupuncture and cupping therapy were applied to treat 60 cases of bronchial asthma.The report is as follows.

  11. Effect of COPD treatments on MRP1-mediated transport in bronchial epithelial cells

    Directory of Open Access Journals (Sweden)

    Margaretha van der Deen

    2008-10-01

    Full Text Available Margaretha van der Deen1, Sandra Homan1, Hetty Timmer-Bosscha1, Rik J Scheper2, Wim Timens3, Dirkje S Postma4, Elisabeth G de Vries1Departments of 1Medical Oncology, 3Pathology, 4Pulmonary Diseases, University Medical Center Groningen and University of Groningen, The Netherlands; 2Department of Pathology, VU University Medical Center, Amsterdam, The NetherlandsBackground: Smoking is the principle risk factor for development of chronic obstructive pulmonary disease (COPD. Multidrug resistance-associated protein 1 (MRP1 is known to protect against toxic compounds and oxidative stress, and might play a role in protection against smoke-induced disease progression. We questioned whether MRP1-mediated transport is influenced by pulmonary drugs that are commonly prescribed in COPD.Methods: The immortalized human bronchial epithelial cell line 16HBE14o- was used to analyze direct in vitro effects of budesonide, formoterol, ipratropium bromide and N-acetylcysteine (NAC on MRP1-mediated transport. Carboxyfluorescein (CF was used as a model MRP1 substrate and was measured with functional flow cytometry.Results: Formoterol had a minor effect, whereas budesonide concentration-dependently decreased CF transport by MRP1. Remarkably, addition of formoterol to the highest concentration of budesonide increased CF transport. Ipratropium bromide inhibited CF transport at low concentrations and tended to increase CF transport at higher levels. NAC increased CF transport by MRP1 in a concentration-dependent manner.Conclusions: Our data suggest that, besides their positive effects on respiratory symptoms, budesonide, formoterol, ipratropium bromide, and NAC modulate MRP1 activity in bronchial epithelial cells. Further studies are required to assess whether stimulation of MRP1 activity is beneficial for long-term treatment of COPD.Keywords: bronchus epithelium, COPD, drugs, MRP1, multidrug resistance, oxidative stress

  12. Chinese and Uighur medicine diagnostic criteria of the evaluation of the Modern drug treatment side-effects in bronchial asthma patients

    Institute of Scientific and Technical Information of China (English)

    Dubrovin Denis; Igor Gogol; Mikhail Baranov; Nurmuhammat Amat; Halmurat Upur

    2014-01-01

    We studied Chinese and Uighur medicines and create an automated computer diagnostics system according to principals of Uighur medicine for evaluation of bronchial asthma patient′s state .498 patients with bronchial asthma were enrolled the automated computer diagnostic program .304 patients were evalu-ated in the process of drug and non-drug treatment .Savda asthma type of Uighur medicine do not corre-sponds with any of the defined clinico-pathogenetic variants of the disease .Thus ,prevailing of atopic bron-chial asthma with probability of 0 .7 is defined with Savda categories with such as ‘Lungs energy deficien-cy’ ,‘Spleen energy deficiency’ and ‘Kidneys Yin deficiency’ (P <0 .01) variants in Chinese Medicine . The combination of infectious-dependent variant of bronchial asthma Abnormal Savda syndrome with atopy with a probability of 0 .8 is accompanied by the categories of ‘Hot and Full’ asthma with such variants as‘Lungs energy deficiency’ ‘Spleen energy deficiency’ ,‘Kidneys Yin deficiency’ (P < 0 .01) .Patients with an easier case of Abnormal Savda syndrome bronchial asthma in 71% of cases have prevailing diagnos-tic categories of ‘External ,Full and Cold’ asthma .Patients with a harder case of Abnormal Savda in 74%of cases belongs to the categories of ‘Internal ,Empty and Hot’ asthma .So ,hormone dependency of the disease in 69% of cases is accompanied by the ‘Kidneys Yin deficiency’ variant and ,if there is an aspirin component in the pathogenesis of mixed asthma ,in 83% of cases ‘Kidneys Yin deficiency’ and ‘Kidneys Yang deficiency’ variants are defined .

  13. Physical conditioning programme for children with bronchial asthma.

    Science.gov (United States)

    Olivia, C K

    1990-04-01

    Exercise-induced bronchospasm limits physical activity in most asthmatic children. Twelve children with stable chronic asthma were enrolled in a physical conditioning program to improve ventilation mechanics and to promote physical activity. The program consisted of eight weekly sessions of one-and-a-half hours each, followed by three months of weekly swimming lessons. The activities were preceded by a warm-up period and interspersed with rest. Premedication with an aerosol bronchodilator or sodium cromoglycate was allowed before training. Cardiorespiratory status was studied before and after the program. The program was well received by the children with no bronchospasm. Five showed improvement in lung volumes and/or flow rates. Bronchial liability remained the same. The basal heart rate slowed in four children. No significant arrhythmia was detected. With continued practice, cardiorespiratory function might improve further. The program should be incorporated as part of the overall management of bronchial asthma. PMID:2116067

  14. Pulmonary ventilation and perfusion scintigraphy in patients with bronchial asthma

    International Nuclear Information System (INIS)

    Pulmonary ventilation and perfusion scan using Xe-133 gas and Tc-99m MAA were performed in 18 patients with bronchial asthma to evaluate the regional pulmonary function. The scintigraphic findings were compared with the results of the auscultation and the conventional pulmonary functioning examination (%FVC, %FEV1.0). Ventilation image showed abnormality in 12 (70.6%) out of the asymptomatic 17 patients and perfusion image showed abnormality in 7 (41.2%) out of 17 patients. These 7 patients with abnormality on perfusion image all showed abnormality on ventilation image. The grade of abnormality in scintigraphic findings was compatible with the values of %FVC and %FEV1.0. In conclusion Xe-133 ventilation and Tc-99m MAA perfusion scan were useful procedures to estimate the pulmonary function of patients with bronchial asthma. (author)

  15. Reviewing bronchial asthma and its pharmacotherapy.

    Science.gov (United States)

    Bardana, E J; Andrasch, R H

    1983-08-01

    Aspirin idiosyncrasy must be watched for, particularly in the elderly, where nonsteroidal anti-inflammatory drugs (NSAIDs) are frequently used for osteoarthritis, bursitis, and related musculoskeletal disorders. An electrocardiogram is advised to exclude or define underlying cardiac disease in elderly asthmatics. They may suffer cardiopulmonary reverses that invariably are perceived as acute asthma by the patient. PMID:6873636

  16. STAT6 EXPRESSION BY PERIPHERAL BLOOD LYMPHOCYTES IN BRONCHIAL ASTHMA

    OpenAIRE

    Mineev, V.N.; L. N. Sorokina

    2014-01-01

    Abstract. The aim of present study was to determine the features of STAT6 and phospho-STAT6 (pSTAT6) expression in bronchial asthma (BA). Patients and methods. Eleven patients with allergic (atopic) steroidfree were examined, five healthy controls served as a control. Expression of proteins (STAT6 and pSTAT6) in peripheral blood lymphocytes was studied by Western blot analysis after cell lysis. Preparation of cell lysates and Western blotting were performed using a standard procedure (Amersha...

  17. Use motion games in exercise with children with bronchial asthma

    Directory of Open Access Journals (Sweden)

    Viktoriya Polkovnyk-Markova

    2016-02-01

    Full Text Available Purpose: to analyze the possibility of using moving games in the rehabilitation of children with bronchial asthma. Material & Methods: the modern scientific literature on integrated prevention and treatment of children with asthma. Results: A high frequency of morphological and functional deviations at children with asthma. Classification and examples of mobile games, which can be used for this group of children. Conclusions: the results of modern research that show the effectiveness the use of physical rehabilitation, including moving games.

  18. Aeroallergen sensitivity among patients suffering from bronchial asthma in Bangalore

    Directory of Open Access Journals (Sweden)

    Giriyanna Gowda

    2013-01-01

    Full Text Available Background: Asthma is a serious public health problem throughout the world and its prevalence has increased in last 2-3 decades. Allergens are one of the many factors which trigger an attack of asthma. Skin prick test is useful in identifying the offending allergen in bronchial asthma. Aim: To identify the possible offending allergens in patients of bronchial asthma. Materials and Methods: The study was a descriptive study conducted at allergy center, Kempegowda Institute of Medical Sciences Hospital and Research Center, Bangalore from January to December 2011. Skin prick test was done in 139 patients suffering from bronchial asthma using 49 allergens extracts. Statistical Analysis: Frequency, proportions, Chi-square test, odds ratio, and 95% confidence interval was used. Results: Out of 139 patients who underwent the skin prick tests, 40% (56 were males and 60% (83 were females. Majority, that is, 60% were in the age group of 21-40 years. Forty-three percent (60 had family history of asthma/atopy, 80% (111 had allergic rhinitis, 24% (34 had chronic urticaria, and 24% (33 had allergic conjunctivitis. Out of 139 patients, 100 (71.94% were sensitive for one or more allergens. The common offending allergens found in the study were dust mites (Dermatophagoides farinae and D. pteronyssinus (DF and DP - 49.28%, dusts - 7.2%, pollens - 6.77%, insects - 6.62%, fungi - 4.53%, and epithelia - 1.92%. Conclusion: The most common allergens in bronchial asthma were dust mites followed by dusts and pollens. Identifying possible allergens in asthma patients help in allergen avoidance and immunotherapy in these patients.

  19. Histamine bronchial challenge: effect on regional ventilation and aerosol deposition.

    OpenAIRE

    Clague, H.; Ahmad, D.; Chamberlain, M. J.; Morgan, W K; Vinitski, S

    1983-01-01

    We studied regional changes in ventilation and aerosol deposition after histamine challenge in six patients with asthma and two with rhinitis and a history of wheezing. All were known to have bronchial hyperreactivity and all showed an increased response to histamine. Ventilation and aerosol deposition studies, using xenon-133 and an aerosol of sulphur colloid tagged with technetium 99m, were performed while they were sitting. Before administration of histamine radioaerosol scintiscans were a...

  20. Bronchial Hyperreactivity in Non-Cystic Fibrosis Bronchiectasis

    OpenAIRE

    Benan Müsellim

    2013-01-01

    Aim: Bronchiectasis is an abnormal dilatation of bronchi and bronchioles due to repeated cycles of airway infection and inflammation. There is a limited data that support the existence of bronchial hyperreactivity (BHR) in bronchiectasis. In the present study we investigated the existence of BHR and possible factors that could affect the BHR in bronchiectasis patients. Matherial and Method: Study performed in bronchiectasis outpatient clinic. We included 69 patients in which the diagnosis of ...

  1. Measurement of the thickness of the bronchial epithelium

    International Nuclear Information System (INIS)

    Cancer of the lung in uranium miners is thought to be related to the inhalation of gaseous radon daughters which become attached to molecules of water vapour or to dust particles. Since, the depth of tissue penetration by alpha particles is short, the thickness of the epithelium that lines the bronchial tree may be a critical factor in the development of cancers at specific sites in the lung. The objectives of the present study were: 1) to measure the thickness of human bronchial epithelium; 2) to determine the distribution and depth of the nuclei of basal cells in the bronchial epithelium; and 3) to compare these parameters in groups of smokers and non-smokers. Twenty-nine surgically removed specimens of the lung were examined (26 smokers, 3 non-smokers). The specimens were fixed and prepared for examination by light and electron microscopy. Blocks of tissue were oriented so that the maximum number of bronchi were cut in cross-section; measurements included bronchi of all sizes from bronchial generations (1≥ 9.01 mm) diameter to the smallest bronchioles, generations 7 - 16 (0.26 - 2.0 mm). Comparison of measurements in smokers and non-smokers show no significant differences, so that the 29 cases are considered to represent a homogeneous group. With progressive divisions of the bronchi, the epithelium decreases in thickness. Of more importance are the figures relating to the distance from the cell surface to the underlying nucleus. Here too, with the exception of goblet cells, the measurements are significantly smaller in generations 7 - 16 than in generation 1

  2. Sleeve resection for delayed presentation of traumatic bronchial transection.

    LENUS (Irish Health Repository)

    Mohamed, H Y

    2010-02-01

    Tracheobronchial disruption is uncommon in blunt chest trauma. Many of these patients die before reaching the hospital. In the majority of survivors diagnosis is occasionally delayed resulting in complications like airway stenosis and lung collapse. Thus it is important to have radiological follow up after severe thoracic trauma. Sleeve resection can be an excellent option to conserve lung tissue in delayed presentation of bronchial transection.

  3. Plasma catecholamines during exercise-induced bronchoconstriction in bronchial asthma.

    OpenAIRE

    Zieliński, J; Chodosowska, E; Radomyski, A; Araszkiewicz, Z; Kozlowski, S

    1980-01-01

    Plasma levels of adrenaline and noradrenaline during and after submaximal exercise in patients with bronchial asthma were investigated. Three groups were studied comprising 10 patients with exercise-induced bronchoconstriction (EIB), 10 asthmatic patients without EIB and four normal control subjects. Plasma catecholamines were measured at rest, at the end of exercise, and five and 15 minutes after exercise. Changes in airway resistance were assessed by measuring peak expiratory flow rate. Sig...

  4. Multipotent Capacity of Immortalized Human Bronchial Epithelial Cells

    OpenAIRE

    Delgado, Oliver; Kaisani, Aadil A.; Spinola, Monica; Xie, Xian-Jin; Batten, Kimberly G.; Minna, John D.; Wright, Woodring E; Shay, Jerry W.

    2011-01-01

    While the adult murine lung utilizes multiple compartmentally restricted progenitor cells during homeostasis and repair, much less is known about the progenitor cells from the human lung. Translating the murine stem cell model to humans is hindered by anatomical differences between species. Here we show that human bronchial epithelial cells (HBECs) display characteristics of multipotent stem cells of the lung. These HBECs express markers indicative of several epithelial types of the adult lun...

  5. SURGICAL TREATMENT AND PROGNOSIS OF BRONCHIAL CARCINOID TUMOR

    Institute of Scientific and Technical Information of China (English)

    张志庸; 李单青; 戈烽; 李泽坚; 孙成孚; 徐乐天; 张士农

    1996-01-01

    In order to understand the effect of surgery in the treatment of the bronchial carcinoid tumor and thefactors affecting prognosis, 18 cases of bronchial carcinoid tumor are presented, including 5 cases withCushing's syndiome. There were Iobectoray in 10, lung wedge resection in 3, excision of intraluminal tumor of bronchus in 3, exploratory thoracotomy in 2 cases.No operation death.Pathological examinstion revealed 14 cases were typical carcinoid tumor and 4 cases were atypical carcinoid tumor.By 2-13 years fol-low-up,3,5 and 10 years survival rate were 82%,78% and 70% respectively.Bronchial carcinoid tumor is often confused microscopic and immunohistochemistry studies.Those patients accompanied with ectopicACTH secretion always have Cushing''s syndrome,resection of tumor can produce good result.Proper operation method depnds on the location of the tumor and patient''s extent of cardiac and pulmonary peserve.Atypical carcinoid tumor had high malignancy and poor prognosis.The size of tumor,lymph node involve ment and adjuvant therapy seem no definite effect on the patients'' survival rate.

  6. Aerosol lung inhalation scintigraphy in children with bronchial asthma

    International Nuclear Information System (INIS)

    Aerosol lung inhalation scintigraphies performed on 37 children with bronchial asthma during asymptomatic periods were evaluated. The findings of their aerosol lung inhalation scintigrams were classified into 4 patterns, as type I: homogeneous distribution without hot spot formation, type II: peripheral homogeneity with central hot spot formation, type IIID (-): inhomogeneous distribution with hot spot formation, but without defect, and type IIID (+): with defect. These aerosol patterns were compared with those of previously reported adult cases and with the severity of bronchial asthma. Normal pattern of type I was found in 5 cases (12%) of our infantile asthmatics in contrast to previously reported adult cases, in which none of normal pattern was found. There were differences between type II and type III in both distribution and disappearance time of hot spot, which indicated that the two types differed from each other in radioaerosol deposition mechanism. There was no significant correlation between type I and type II in the severity of asthma and the frequency of asthmatic attack. Type II may be clinically considered to be the same type as type I. There is the statistically significant difference between type I, II and type III in the frequency of asthmatic attack, but not in the severity of asthma, although most of serious cases showed type III. Aerosol lung inhalation scintigraphy is a useful examination for children with bronchial asthma in which lung function tests may be difficult to perform. (author)

  7. 支气管哮喘的免疫疗法%Immunologic therapy for bronchial asthma

    Institute of Scientific and Technical Information of China (English)

    康军英; 王红

    2008-01-01

    支气管哮喘(简称哮喘)是常见的呼吸系统疾病.随着对哮喘发病机制的了解,哮喘的治疗已取得很大进展,针对免疫机制的治疗是哮喘治疗的重要方面.本文重点阐述特异性免疫治疗、免疫球蛋白E单克隆抗体、细胞因子调节剂在哮喘方面的治疗作用.%Bronchial asthma(asthma)is a normal disease of respiratory system.With understanding of asthmatic pathogenesis,asthmatic therapy has tremendous progress,to aim directly at immunologic mechanism therapy is an important respect.This text mainly explains the treatment of specific immunity therapy,IgE monoclonal antibody,cytokine moderator therapeutic on asthma.

  8. [THE GENETIC EXAMINATION OF BRONCHIAL LAVAGE ENABLES THE PROMPT DIAGNOSIS OF PULMONARY MYCOBACTERIUM KANSASII--A CASE REPORT].

    Science.gov (United States)

    Mori, Masahide; Ageshio, Fumitaka; Kagawa, Hiroyuki; Oshitani, Yohei; Fujikawa, Takeya; Saito, Haruko; Sako, Hajime; Yano, Yukihiro; Kitada, Seigo; Maekura, Ryoji

    2015-08-01

    A 59-year-old man with chronic obstructive pulmonary disease and bronchial asthma presented at our hospital with an abnormal shadow on the chest radiograph, which was obtained as part of a routine medical examination. Computed tomography of the chest revealed two nodules in the right upper lung with the longest diameter measuring 29 mm and 10 mm, respectively. A granulomatous disease was strongly suspected based on the histological features of the transbronchial lung biopsy specimen. Results of smear examination for mycobacteria and genetic examination of the bronchial lavage aspirate by the transcription reverse transcription concerted (TRC) reaction method for Mycobacterium tuberculosis and M. avium complex (MAC), were both negative. However, three days after the bronchoscopic examination, an additional genetic examination by the TRC method confirmed the diagnosis of M. kansasii infection. About two weeks later, the culture results were positive and M. kansasii infection was re-confirmed with the DNA probe method. The patient responded well to treatment with a combination of isoniazid, rifampicin, and ethambutol. In Japan, among the nontuberculous mycobacterial infections, the prevalence of pulmonary M.kansasii disease is second only to infection with MAC. However, it is often difficult to distinguish this disease from pulmonary tuberculosis. In this patient, a genetic examination with the TRC method enabled a prompt diagnosis of M. kansasii infection. The TRC method appears to be a useful tool for diagnosing nontubercular mycobacterial infections. PMID:26665518

  9. Using the capnograph to confirm lung isolation when using a bronchial blocker.

    Science.gov (United States)

    Fisicaro, Marc D; Maguire, David P; Armstead, Valerie E

    2010-11-01

    The endotracheal tube and bronchial blocker combination is an accepted lung isolation technique used during thoracic surgery. A reliable and inexpensive method of confirming lung isolation that uses capnographic monitoring of the bronchial blocker central lumen is presented. As the bronchial blocker balloon is inflated, lung isolation is confirmed when the normal respiratory variation of carbon dioxide (CO(2)) is replaced by a persistent plateau CO(2) waveform. PMID:21056815

  10. Bronchial asthma control after argon plasma coagulation turbinectomy in patients with chronic rhinitis

    OpenAIRE

    Jura-Szołtys, Edyta; Ficek, Rafał; Ficek, Joanna; Markowski, Jarosław; Chudek, Jerzy

    2013-01-01

    Bronchial asthma is frequently accompanied by chronic rhinitis. It has been observed that effective treatment of rhinitis may reduce asthma symptoms. The aim of the study was the evaluation of the control of bronchial asthma symptoms in patients with chronic rhinitis after argon plasma coagulation turbinectomy (APCt). The effect of APCt was assessed in 47 adults with drug-resistant chronic rhinitis and bronchial asthma 3-month post-procedure. Changes of asthma symptoms were scored using Asthm...

  11. The clinical significance of the substance P in bronchoalveolar lavage fluid in patients with bronchial asthma

    International Nuclear Information System (INIS)

    Using radioimmunoassay to measure the substance P (SP) in bronchoalveolar lavage fluid in thirty patients with bronchial asthma and thirty healthy persons. Compered with healthy group (33.4±24.5 pmol/L), the SP in bronchial asthma group (240.2±18.7 pmol/L) increased significantly (p < 0.01). SP may play a role in the development of bronchial asthma

  12. Bronchial Responsiveness in Patients with Restrictive Spirometry

    OpenAIRE

    Keddissi, Jean I.; Elya, Marwan K.; Farooq, Saif U.; Youness, Houssein A.; Kellie R. Jones; Ahmed Awab; Kinasewitz, Gary T.

    2013-01-01

    Background. Improvement in PFT after bronchodilators is characteristic of obstructive airway diseases such as COPD. However, improvement in patients with restrictive pattern is occasionally seen. We aim to determine the clinical significance of a bronchodilator responsive restrictive defect. Methods. Patients with restrictive spirometry and a bronchodilator study were identified at the University of Oklahoma and Oklahoma City VAMC between September 2003 and December 2009. Restriction was defi...

  13. Acute toxicity of silver and carbon nanoaerosols to normal and cystic fibrosis human bronchial epithelial cells.

    Science.gov (United States)

    Jeannet, Natalie; Fierz, Martin; Schneider, Sarah; Künzi, Lisa; Baumlin, Nathalie; Salathe, Matthias; Burtscher, Heinz; Geiser, Marianne

    2016-01-01

    Inhalation of engineered nanoparticles (NP) poses a still unknown risk. Individuals with chronic lung diseases are expected to be more vulnerable to adverse effects of NP than normal subjects, due to altered respiratory structures and functions. Realistic and dose-controlled aerosol exposures were performed using the deposition chamber NACIVT. Well-differentiated normal and cystic fibrosis (CF) human bronchial epithelia (HBE) with established air-liquid interface and the human bronchial epithelial cell line BEAS-2B were exposed to spark-generated silver and carbon nanoaerosols (20 nm diameter) at three different doses. Necrotic and apoptotic cell death, pro-inflammatory response, epithelial function and morphology were assessed within 24 h after aerosol exposure. NP exposure resulted in significantly higher necrosis in CF than normal HBE and BEAS-2B cells. Before and after NP treatment, CF HBE had higher caspase-3 activity and secreted more IL-6 and MCP-1 than normal HBE. Differentiated HBE had higher baseline secretion of IL-8 and less caspase-3 activity and MCP-1 secretion compared to BEAS-2B cells. These biomarkers increased moderately in response to NP exposure, except for MCP-1, which was reduced in HBE after AgNP treatment. No functional and structural alterations of the epithelia were observed in response to NP exposure. Significant differences between cell models suggest that more than one and fully differentiated HBE should be used in future toxicity studies of NP in vitro. Our findings support epidemiologic evidence that subjects with chronic airway diseases are more vulnerable to adverse effects of particulate air pollution. Thus, this sub-population needs to be included in nano-toxicity studies.

  14. Peculiarities of cardiac performance in children with bronchial asthma against chronic tonsillitis

    Directory of Open Access Journals (Sweden)

    Kondratiev V.А.

    2015-11-01

    Full Text Available A comparative assessment of cardiac performance by the data of clinical-instrumental examination of 50 patients aged 5-15 years with persisting form of partially-controlled atopic bronchial asthma in the period between the attacks of the disease was made. Children were divided into two groups depending on presence of concomitant chronic tonsillitis (18 patients or absence of the latter (32 patients. In all cases by the data of spirography in children against chronic tonsillitis in the period between the attacks of the disease there were revealed ventillation disorders in the lungs by obstruction type, this promoted elevation of pressure in the pulmonary artery. Only in this group of patients in 27,8% of cases pulmonary arterial hypertension of moderate severity was revealed. By echocardiography data in children with asthma against chronic tonsillitis reliably more often (р<0,01 there was revealed decrease of contractile function of myocardium of the left cardiac ventricle; this was caused by the presence of metabolic disturbances in the myocardium in the majority of cases (83,3%. These changes were registered on the electrocardiogram in the form of repolarisation changes of the ventricular complex. Presence of concomitant chronic tonsillitis in children with bronchial asthma favored formation of pulmonary hypertension on the one side and worsening of contractile ability of myocardium of the left cardiac ventricle on the other side; this required performing therapeutic-preventive measures for sanation of chronic infectious focus in the nasopharynx to prevent formation of chronic cardiac insufficiency during asthma course.

  15. Perception of bronchial obstruction in asthmatic patients. Relationship with bronchial eosinophilic inflammation and epithelial damage and effect of corticosteroid treatment.

    OpenAIRE

    Roisman, G L; Peiffer, C; Lacronique, J. G.; Le Cae, A; Dusser, D J

    1995-01-01

    We studied the perception of bronchoconstriction in asthmatic subjects who were randomly treated with inhaled beta 2 agonist given either alone (n = 9) or associated with inhaled corticosteroids (n = 9). Methacholine and bradykinin challenges, bronchoalveolar lavage, and bronchial biopsies were performed in all subjects. After each dose of agonist, breathlessness was assessed using a visual analog scale (VAS) and the forced expiratory volume in 1 s (FEV1) was measured. The relationship betwee...

  16. Analysis of the dynamic states of pulmonary ventilation and perfusion in bronchial asthmatics using 133Xe gas ventilation scintigraphy and 99mTc-MAA lung perfusion scintigraphy

    International Nuclear Information System (INIS)

    To clarify the organic changes and pathophysiology of the lungs in intractable asthmatics, the dynamic states of pulmonary ventilation and perfusion were analyzed in 15 bronchial asthmatics in the stable state using 133Xe gas ventilation scintigraphy and 99mTc-MAA lung perfusion scintigraphy, respectively. Dysfunction of pulmonary ventilation and impairment of pulmonary perfusion were significantly severer in intractable asthmatics than in non-intractable asthmatics (p<0.01, p<0.05, respectively) and those of asthma caused by aging. Late onset asthmatics with a long duration of disease tended to show severe impairment of pulmonary perfusion. Above all, the longer the duration of the disease, the severer became the impairment of pulmonary perfusion in late onset intractable asthmatics (LOIA). These findings suggest that the progression of irreversible organic changes of lungs in LOIA is involved in the severity of bronchial asthma. (author)

  17. Control Method and the Disease Factors Influencing Compliance of Inhaled Corticosteroids in Patients with Bronchial Asthma%支气管哮喘患者吸入糖皮质激素依从性影响因素及病情控制对策

    Institute of Scientific and Technical Information of China (English)

    孙彩

    2015-01-01

    Objective To analyze patients with bronchial asthma inhaled corticosteroids compliance factors,provide the basis for clinical ef ective control of the il ness.Methods Select our hospital during 2013~2014 treated 120 cases of bronchial asthma and proves the patients treated with inhaled corticosteroids as the research object,to inhaled corticosteroid treatment adherence influence factors were analyzed.Results The study of 120 cases with 24 patients adhere to inhaled corticosteroids,the compliance rate was 20%,the factors influencing patients adhere to inhaled corticosteroid therapy are mainly:hormone fear,expensive,and asthma is longer than the lack of knowledge of prevention and cure,treatment is dif icult to insist to use,etc.Conclusion Ef ect of inhaled corticosteroids in patients with bronchial asthma treatment factors varied,improve patients medication compliance is the key to strengthen health education,psychological nursing,inhaled corticosteroids behavior of specific interventions,and improve the family support,etc.%目的分析支气管哮喘患者吸入糖皮质激素依从性影响因素,为临床有效控制病情提供依据。方法选择我院在2013年~2014年收治的120例支气管哮喘并具备吸入糖皮质激素治疗指证的患者为研究对象,对吸入激素治疗依从性影响因素进行统计分析。结果本次研究的120例患者中有24例坚持吸入糖皮质激素,依从率为20%,影响患者坚持吸入激素治疗的因素主要为:激素恐惧、价格昂贵、哮喘防治知识缺乏、疗程较长难以坚持使用等。结论影响支气管哮喘患者吸入糖皮质激素治疗的因素多种多样,提高患者用药依从性的关键在于加强健康教育、心理护理、吸入激素行为的具体干预以及提高家庭支持等。

  18. Allergen-induced bronchial hyperreactivity and eosinophilic inflammation occur in the absence of IgE in a mouse model of asthma

    OpenAIRE

    Mehlhop, Paul D.; van de Rijn, Matthijs; Goldberg, Alison B.; Brewer, Joanne P.; Kurup, Viswanath P.; Martin, Thomas R.; Oettgen, Hans C.

    1997-01-01

    In patients with asthma, elevations of IgE correlate both with allergic inflammation of the airways and with bronchial hyperreactivity (BHR). Several investigations, using mouse models of this disease, have indicated a central role for IgE in the pathogenesis of the eosinophilic inflammation as well as in the obstructive airway physiology of BHR. Some diagnostic studies and therapeutic strategies for asthma are based on the putative role of IgE in asthma pathogenesis. Here, we use mice with a...

  19. 支气管哮喘患者的健康指导%Health education of bronchial asthma patients

    Institute of Scientific and Technical Information of China (English)

    孙秀玲; 王勤颖; 赵晓梅; 董书宇

    2012-01-01

    Objective: Improve the patients with bronchial asthma disease cognition level and self management ability, improve the therapeutic effect and life quality. Methods: In 56 cases with bronchial asthma patients in routine nursing was carried out on the basis of a health education intervention, including psychological guidance, diet guidance, drug guidance, self care knowledge guidance, predisposing factors prevention. Results: Patient health education knowledge level and the quality of nursing satisfaction improved significantly, hospitalization time was obviously shortened. Conclusion: Strengthen health education to enhance the awareness of self health care of bronchial asthma patients, shorten the course of disease, relapse prevention, improve pan'ents'quality of life and satisfaction with nursing care has important significance.%目的:提高支气管哮喘患者对疾病的认知程度和自我管理能力,提高治疗效果和生活质量.方法:对住院确诊的56例支气管哮喘患者进行健康教育,包括心理指导、饮食指导、药物指导,自我护理知识指导、诱发因素预防等.结果:患者健康教育知识掌握程度和护理质量满意度明显提高,住院时间显著缩短.结论:加强健康教育,对于增强支气管哮喘患者的自我保健意识,缩短病程,预防复发,提高患者生活质量具有重要意义.

  20. Role of Lyn kinase and endoplasmic reticulum stress in allergic bronchial asthma%Lyn激酶和内质网应激在过敏性支气管哮喘中的作用

    Institute of Scientific and Technical Information of China (English)

    伍娟; 杨小琼

    2016-01-01

    Bronchial asthma is an airway inflammatory disease which many kinds of cells and cellular compo-nents are involved in. It is characterized by airway inflammation, airway remodeling and airway hyperresponsiveness. However, the pathogenesis of bronchial asthma is very complex. Therefore in this paper, the important roles of Lyn ki-nase and endoplasmic reticulum stress in the pathogenesis of bronchial asthma are reviewed.%支气管哮喘是多种细胞及细胞组分参与的气道炎症性疾病,主要以气道炎症和气道重塑、气道高反应性为特征;然而其发病机制十分复杂,本文对Lyn激酶和内质网应激在支气管哮喘的发病机制中的重要作用进行综述.

  1. Long-term outcomes of the bronchial artery embolization are diagnosis dependent

    Directory of Open Access Journals (Sweden)

    Vikas Pathak

    2016-01-01

    Full Text Available Background: Bronchial artery embolization (BAE is an established, safe, and effective procedure for the treatment of hemoptysis but long-term outcomes of the BAE have never been investigated before. Objectives: To retrospectively analyze long-term outcomes of the BAE. Materials and Methods: A retrospective chart analysis was done from the hospital central database for all patients undergoing the BAE over a consecutive 14-year period (January 2000-February 2014. A total of 58 patients were identified from the database. Eight patients were excluded due to the lack of follow-up. Data such as patient demographics, reason for hemoptysis, medical imaging results, bronchoscopy findings, recurrence rates, and morbidity/mortality rates after the BAE were collected. Results: Eighty three embolizations were performed in 50 patients. The median follow-up was of 2.2 years. Cystic fibrosis (CF bronchiectasis was the most common etiology (21/50, followed by non-CF bronchiectasis (9/50. Cavitary lung disease occurred in 12/50 patients, an additional 4/50 had cancer (primary lung and metastatic, and one patient had antineutrophil cytoplasmic antibody (ANCA vasculitis. In three patients the etiology was unknown. Postprocedural complications occurred in 5/83 (6% patients, two patients with two major complications - stroke (one and paraplegia (one - and three patients with minor complications - chest pain (two and bronchial artery dissection (one. A total of 15/50 patients died during the follow-up. Three patients died of hemoptysis, and the remaining deaths were unrelated to the procedure or hemoptysis. Twenty four patients had recurrent hemoptysis. A Kaplan-Meier analysis revealed an excellent long-term survival that was 85% at 10 years. Conclusions: The BAE is a safe and effective procedure with excellent overall long-term survival.

  2. Chronic respiratory symptoms, bronchial responsiveness and dietary sodium and potassium: a population-based study.

    Science.gov (United States)

    Zoia, M C; Fanfulla, F; Bruschi, C; Basso, O; De Marco, R; Casali, L; Cerveri, I

    1995-04-01

    A possible relationship between Na+ intake and increased prevalence and mortality from asthma and chronic obstructive pulmonary disease (COPD) has been suggested but not clearly proven for several reasons (difficulty in assessing Na+ and K+ both by 24 h excretion and dietary recall, too small an effect of these ions on the pathology, and the role of potential confounders). We wanted to determine the relationship of Na+ and K+ intake, assessed by means of a 7 day recall, with chronic respiratory symptoms and bronchial responsiveness in a sample of the general population. Two hundred and five subjects were studied, with complete dietary and respiratory questionnaires, and baseline respiratory function tests, together with a subsample of 146 subjects who underwent histamine challenge. The 7 day recall consisted of two parts: the first assessed discretionary Na+; and the second assessed Na+ and K+ contained in food. The whole sample was split into two groups based on the levels of consumption, and the statistical analysis was performed contrasting the three lower quartiles vs the highest. Smoking habit, social economic status, age and body mass index (BMI) were not confounders for Na+ and K+ intake. The prevalence of symptomatic subjects and baseline respiratory function values were not significantly different in the two groups of quartiles for Na+ and K+. Baseline respiratory values and dose-response slope of the subsample were also not significantly different. We did not prove a relationship between these dietary factors and either bronchial responsiveness or chronic respiratory symptoms. Although we consider that our questionnaire is more reliable than other methods for Na+ and K+ assessment, several potential biases still remain.(ABSTRACT TRUNCATED AT 250 WORDS) PMID:7613539

  3. Ciliary genes are down-regulated in bronchial tissue of primary ciliary dyskinesia patients.

    Directory of Open Access Journals (Sweden)

    Maciej Geremek

    Full Text Available Primary ciliary dyskinesia (PCD is a rare, genetically heterogeneous disease characterized by recurrent respiratory tract infections, sinusitis, bronchiectasis and male infertility. The pulmonary phenotype in PCD is caused by the impaired motility of cilia in the respiratory epithelium, due to ultrastructural defects of these organelles. We hypothesized that defects of multi-protein ciliary complexes should be reflected by gene expression changes in the respiratory epithelium. We have previously found that large group of genes functionally related to cilia share highly correlated expression pattern in PCD bronchial tissue. Here we performed an explorative analysis of differential gene expression in the bronchial tissue from six PCD patients and nine non-PCD controls, using Illumina HumanRef-12 Whole Genome BeadChips. We observed 1323 genes with at least 2-fold difference in the mean expression level between the two groups (t-test p-value <0.05. Annotation analysis showed that the genes down-regulated in PCD biopsies (602 were significantly enriched for terms related to cilia, whereas the up-regulated genes (721 were significantly enriched for terms related to cell cycle and mitosis. We assembled a list of human genes predicted to encode ciliary proteins, components of outer dynein arms, inner dynein arms, radial spokes, and intraflagellar transport proteins. A significant down-regulation of the expression of genes from all the four groups was observed in PCD, compared to non-PCD biopsies. Our data suggest that a coordinated down-regulation of the ciliome genes plays an important role in the molecular pathomechanism of PCD.

  4. Serial change of atelectasis in acute bronchial obstruction

    International Nuclear Information System (INIS)

    This study evaluated MR imaging (MRI) of atelectatic lungs caused by acute bronchial obstruction. In 12 Japanese white rabbits, sudden obstruction of one main bronchus was induced with compressed polyvinylalcohol sponge plugs, and three rabbits each were sacrificed at 3, 7, 14, and 21 days. Unilateral complete airway obstruction persisted in 8 of the 12 rabbits. After T1: [500/20/2 (TR/TE/excitations), 5 mm thickness, 12 cm FOV] and T2: [2500/80/4, 5 mm thickness, 12 cm FOV] weighted imaging, both lungs and heart were inflated, fixed (Heitzman's method) and embedded en bloc in collodion. MR images of the atelectatic portion were evaluated, and MRI findings of the atelectatic portion were correlated with pathologic findings. MRI, especially T2 weighted images showed some characteristic signal patterns in each period. Correlations of these MRI features were performed with the pathologic findings. The peripheral pulmonary parenchyma, on T2 weighted images showed signal increase with time which was attributed to edematous fluid retention in pulmonary alveoli. The difference between inhomogeneous and homogeneous signal intensities on T2 weighted images on the 7th day was attributed to the patchy and even distribution of inflammatory process in pulmonary parenchyma. On the 21th day, hypointensity bands were observed on T1 and T2 weighted images. These hypointensity bands were attributed to fibrotic changes in bronchial walls and nearby alveolar walls. MR images well demonstrated pathological changes in atelectatic lungs caused by acute bronchial obstruction with time, suggesting the clinical usefulness of this approach. (author)

  5. Lack of Dystrophin Affects Bronchial Epithelium in mdx Mice.

    Science.gov (United States)

    Morici, Giuseppe; Rappa, Francesca; Cappello, Francesco; Pace, Elisabetta; Pace, Andrea; Mudò, Giuseppa; Crescimanno, Grazia; Belluardo, Natale; Bonsignore, Maria R

    2016-10-01

    Mild exercise training may positively affect the course of Duchenne Muscular Dystrophy (DMD). Training causes mild bronchial epithelial injury in both humans and mice, but no study assessed the effects of exercise in mdx mice, a well known model of DMD. The airway epithelium was examined in mdx (C57BL/10ScSn-Dmdmdx) mice, and in wild type (WT, C57BL/10ScSc) mice either under sedentary conditions (mdx-SD, WT-SD) or during mild exercise training (mdx-EX, WT-EX). At baseline, and after 30 and 45 days of training (5 d/wk for 6 weeks), epithelial morphology and markers of regeneration, apoptosis, and cellular stress were assessed. The number of goblet cells in bronchial epithelium was much lower in mdx than in WT mice under all conditions. At 30 days, epithelial regeneration (PCNA positive cells) was higher in EX than SD animals in both groups; however, at 45 days, epithelial regeneration decreased in mdx mice irrespective of training, and the percentage of apoptotic (TUNEL positive) cells was higher in mdx-EX than in WT-EX mice. Epithelial expression of HSP60 (marker of stress) progressively decreased, and inversely correlated with epithelial apoptosis (r = -0.66, P = 0.01) only in mdx mice. Lack of dystrophin in mdx mice appears associated with defective epithelial differentiation, and transient epithelial regeneration during mild exercise training. Hence, lack of dystrophin might impair repair in bronchial epithelium, with potential clinical consequences in DMD patients. J. Cell. Physiol. 231: 2218-2223, 2016. © 2016 Wiley Periodicals, Inc. PMID:26868633

  6. Polymorphisms and Haplotypes of Acid Mammalian Chitinase Are Associated with Bronchial Asthma

    OpenAIRE

    Bierbaum, Sibylle; Nickel, Renate; Koch, Anja; Lau, Susanne; Deichmann, Klaus A.; Wahn, Ulrich; Superti-Furga, Andrea; Heinzmann, Andrea

    2005-01-01

    Rationale: Chitinases are enzymes that cleave chitin, a polysaccharide contained in many parasites of humans. Recent studies in mouse models of bronchial asthma have shown that acid mammalian chitinase (AMCase) is involved in the pathophysiology of asthma. It acts downstream of interleukin-13; inhibition of AMCase leads to an abrogated T-helper cell 2 inflammation, less bronchial hyperreactivity, and fewer eosinophils.

  7. Use of tracheal auscultation for the assessment of bronchial responsiveness in asthmatic children

    NARCIS (Netherlands)

    Sprikkelman, AB; Grol, MH; Lourens, MS; Gerritsen, J; Heymans, HSA; vanAalderen, WMC

    1996-01-01

    Background - It can be difficult to assess bronchial responsiveness in children because of their inability to perform spirometric tests reliably. In bronchial challenges lung sounds could be used to detect the required 20% fall in the forced expiratory volume in one second (FEV(1)). A study was unde

  8. An increase in bronchial responsiveness is associated with continuing or restarting smoking

    NARCIS (Netherlands)

    Chinn, S; Jarvis, D; Luczynska, CM; Ackermann-Liebrich, U; Anto, JM; Cerveri, [No Value; de Marco, R; Gislason, T; Heinrich, J; Janson, C; Kunzli, N; Leynaert, N; Neukirch, FO; Schouten, JP; Sunyer, J; Svanes, C; Wjst, M; Burney, PG

    2005-01-01

    Rationale: Bronchial responsiveness (BHR) has been found to be associated with smoking, atopy, and lower lung function in cross-sectional studies, but there is little information on determinants of change in adults. Objectives: To analyze change in bronchial responsiveness in an international longit

  9. Transverse myelitis: a reversible complication of bronchial artery embolisation in cystic fibrosis

    OpenAIRE

    Fraser, K. L.; Grosman, H.; Hyland, R H; Tullis, D. E.

    1997-01-01

    The case history is presented of a young woman with cystic fibrosis and life threatening haemoptysis. Angiography revealed enlarged bronchial vessels, one of which supplied the contralateral lung. Transverse myelitis developed following bronchial artery embolisation but recovery was rapid and nearly complete. Haemoptysis did not recur during four years of follow up. 




  10. Pulmonary artery--bronchial fistula: a new complication of Swan-Ganz catheterization.

    Science.gov (United States)

    Rubin, S A; Puckett, R P

    1979-04-01

    A patient with a Swan-Ganz catheter developed massive hemoptysis. Injection of radiographic contrast media through the catheter revealed rapid filling of the tracheo-bronchial tree, consistent with direct pulmonary artery-bronchial communication. Development of hemoptysis in a patient with a Swan-Ganz catheter should alert the clinician to this possibility. PMID:446146

  11. Moxifloxacin Penetration in Bronchial Secretions of Mechanically Ventilated Patients with Pneumonia

    OpenAIRE

    Leone, Marc; Albanèse, Jacques; Sampol-Manos, Emmanuelle; Simon, Nicolas; Lacarelle, Bruno; Bruguerolle, Bernard; Martin, Claude

    2004-01-01

    The pharmacokinetics of moxifloxacin was studied in 17 mechanically ventilated patients with pneumonia. Patients were given 400 mg of moxifloxacin intravenously. Blood samples and bronchial secretions were taken on days 1 and 4. A dose of 400 mg of moxifloxacin allows one to achieve efficient concentrations in bronchial secretions and plasma.

  12. Indirect bronchial provocation tests in childhood asthma : Monitoring short-term treatment changes

    NARCIS (Netherlands)

    Kersten, Elin

    2015-01-01

    In this thesis, we used indirect bronchial provocation tests to monitor treatment changes in asthmatic children. Indirect bronchial provocation tests assess the response of the airways to stimuli that act on inflammatory cells present in the airways. The response to indirect stimuli is greater in ch

  13. Bronchial atresia in a neonate with congenital cytomegalovirus infection

    Directory of Open Access Journals (Sweden)

    Abdullah A Yousef

    2013-01-01

    Full Text Available Bronchial atresia (BA is characterized by a mucus-filled bronchocele in a blind-ending segmental or lobar bronchus with hyperinflation of the obstructed segment of the lung. We describe a neonate who presented on his 9 th day of life with respiratory distress. Chest computed tomography showed a soft tissue density involving the right middle lobe (RML. RML lobectomy confirmed the diagnosis of BA. Cytomegalovirus was detected by polymerase chain reaction in blood, urine, and tracheal aspirates which may provide further insight into the pathogenesis of BA.

  14. Alveolar epithelial permeability in bronchial asthma in children

    International Nuclear Information System (INIS)

    To evaluate alveolar epithelial permeability (kep) in children with bronchial asthma, 99mTc-DTPA (diethylene triamine penta acetate) aerosol lung inhalation scintigraphies were performed. There was no correlation between the kep value and the severity of asthma. On the other hand, out of 10 cases which had no aerosol deposition defect in the lung field, 4 showed high kep values on the whole lung field and 7 had high kep value areas, particularly apparent in the upper lung field. These results suggest that even when the central airway lesions are mild, severe damage exists in the alveolar region of the peripheral airway. (author)

  15. Scale dependence of branching in arterial and bronchial trees

    CERN Document Server

    Restrepo, J G; Hunt, B R; Restrepo, Juan G.; Ott, Edward; Hunt, Brian R.

    2005-01-01

    Although models of branching in arterial and bronchial trees often predict a dependence of bifurcation parameters on the scale of the bifurcating vessels, direct verifications of this dependence with data are uncommon. We compare measurements of bifurcation parameters in airways and arterial trees of different mammals as a function of scale to general features predicted by theoretical models. We find that the size dependence is more complex than existing theories based solely on energy minimization explain, and suggest additional factors that may govern the branching at different scales.

  16. The inhibitory mechanism of Cordyceps sinensis on cigarette smoke extract-induced senescence in human bronchial epithelial cells

    Directory of Open Access Journals (Sweden)

    Liu AL

    2016-07-01

    Full Text Available Ailing Liu,1,2,* Jinxiang Wu,1,* Aijun Li,2 Wenxiang Bi,3 Tian Liu,1 Liuzhao Cao,1 Yahui Liu,1 Liang Dong1 1Department of Pulmonary Diseases, Qilu Hospital, Shandong University, Jinan, Shandong, People’s Republic of China; 2Department of Pulmonary Diseases, Weihai Municipal Hospital, Weihai, Shandong, People’s Republic of China; 3Institute of Biochemistry and Molecular Biology, School of Medicine, Shandong University, Jinan, Shandong, People’s Republic of China *These authors contributed equally to this work Objectives: Cellular senescence is a state of irreversible growth arrest induced either by telomere shortening (replicative senescence or stress. The bronchial epithelial cell is often injured by inhaled toxic substances, such as cigarette smoke. In the present study, we investigated whether exposure to cigarette smoke extract (CSE induces senescence of bronchial epithelial cells; and Cordyceps sinensis mechanism of inhibition of CSE-induced cellular senescence.Methods: Human bronchial epithelial cells (16HBE cells cultured in vitro were treated with CSE and/or C. sinensis. p16, p21, and senescence-associated-galactosidase activity were used to detect cellular senescence with immunofluorescence, quantitative polymerase chain reaction, and Western blotting. Reactive oxygen species (ROS, PI3K/AKT/mTOR and their phosphorylated proteins were examined to testify the activation of signaling pathway by ROS fluorescent staining and Western blotting. Then, inhibitors of ROS and PI3K were used to further confirm the function of this pathway.Results: Cellular senescence was upregulated by CSE treatment, and C. sinensis can decrease CSE-induced cellular senescence. Activation of ROS/PI3K/AKT/mTOR signaling pathway was enhanced by CSE treatment, and decreased when C. sinensis was added. Blocking ROS/PI3K/AKT/mTOR signaling pathway can attenuate CSE-induced cellular senescence.Conclusion: CSE can induce cellular senescence in human bronchial

  17. Remission of bronchial asthma after viral clearance in chronic hepatitis C

    Institute of Scientific and Technical Information of China (English)

    Norihiko Yamamoto; Kazumoto Murata; Takeshi Nakano

    2005-01-01

    A 53-year-old man with a history of blood transfusion at the age of 20 was admitted to our hospital because of liver dysfunction. He had bronchial asthma when he was 18 years old, which naturally resolved within 2 years. However, his bronchial asthma recurred at the age of 45 and was treated with oral theophylline. He was diagnosed as having chronic hepatitis C based on the histological and clinical findings, and then interferon (IFN) therapy was administered. The frequency of bronchial asthma attack was gradually decreasing after IFN therapy with marked improvement of hypereosinophilia. He achieved sustained viral response (SVR) and his bronchial asthma did not worsen even after the cessation of IFN. Hepatitis C virus (HCV) infection and IFN therapy were considered in the remission of asthma in this case. HCV infection could be the cause of bronchial asthma, especially in patients with late appearance of asthma.

  18. Adamtsl2 deletion results in bronchial fibrillin microfibril accumulation and bronchial epithelial dysplasia – a novel mouse model providing insights into geleophysic dysplasia

    Directory of Open Access Journals (Sweden)

    Dirk Hubmacher

    2015-05-01

    Full Text Available Mutations in the secreted glycoprotein ADAMTSL2 cause recessive geleophysic dysplasia (GD in humans and Musladin–Lueke syndrome (MLS in dogs. GD is a severe, often lethal, condition presenting with short stature, brachydactyly, stiff skin, joint contractures, tracheal-bronchial stenosis and cardiac valve anomalies, whereas MLS is non-lethal and characterized by short stature and severe skin fibrosis. Although most mutations in fibrillin-1 (FBN1 cause Marfan syndrome (MFS, a microfibril disorder leading to transforming growth factor-β (TGFβ dysregulation, domain-specific FBN1 mutations result in dominant GD. ADAMTSL2 has been previously shown to bind FBN1 and latent TGFβ-binding protein-1 (LTBP1. Here, we investigated mice with targeted Adamtsl2 inactivation as a new model for GD (Adamtsl2−/− mice. An intragenic lacZ reporter in these mice showed that ADAMTSL2 was produced exclusively by bronchial smooth muscle cells during embryonic lung development. Adamtsl2−/− mice, which died at birth, had severe bronchial epithelial dysplasia with abnormal glycogen-rich inclusions in bronchial epithelium resembling the cellular anomalies described previously in GD. An increase in microfibrils in the bronchial wall was associated with increased FBN2 and microfibril-associated glycoprotein-1 (MAGP1 staining, whereas LTBP1 staining was increased in bronchial epithelium. ADAMTSL2 was shown to bind directly to FBN2 with an affinity comparable to FBN1. The observed extracellular matrix (ECM alterations were associated with increased bronchial epithelial TGFβ signaling at 17.5 days of gestation; however, treatment with TGFβ-neutralizing antibody did not correct the epithelial dysplasia. These investigations reveal a new function of ADAMTSL2 in modulating microfibril formation, and a previously unsuspected association with FBN2. Our studies suggest that the bronchial epithelial dysplasia accompanying microfibril dysregulation in Adamtsl2−/− mice

  19. [Some modern views of the role of the eosinophils in allergic reactions and bronchial asthma and a new method of detecting eosinophils in the bronchial secretion].

    Science.gov (United States)

    Denchev, K; Lipcheva, N; Kis'ova, K

    1976-01-01

    A review of certain contemporary opinions of eosinophil function in allergic reactions and bronchial asthma is presented in this report. Phagocytosis and processing of the complexes antigenantibody, histamine inhibition and a histamine elimination elimination by a specific inhibitor, isolated from eosinophilis (EDI), stimulation of prostaglandines E release, which also inhibit histamine and have a bronchial dilataion effect. The new method is recommended for eosinophil detection in sputa based on the fluorescent principle as faster and more efficient.

  20. Quantitative bronchial luminal volumetric assessment of pulmonary function loss by thin-section MDCT in pulmonary emphysema patients

    International Nuclear Information System (INIS)

    Objectives: To determine the capability of quantitative bronchial luminal volume to assess pulmonary function loss and disease severity in pulmonary emphysema patients. Methods: Thirty-seven smokers (mean age, 68.1 years) underwent CT examinations and pulmonary function tests. For the quantitative assessment, luminal voxels of trachea and bronchi were computationally counted and the ratio of the following luminal voxels to all luminal voxels was obtained: (1) the lobe bronchi and the peripheral bronchi (Ratiolobe), and (2) the main bronchi and the peripheral bronchi (Ratiomain). To determine the capability of these assessments to predict pulmonary function loss, these ratios were correlated with pulmonary function tests. To determine the capability for predicting disease severity, these ratios were compared between clinical groups. Results: These ratios were no significant correlated with vital capacity and forced vital capacity (FVC) (p > 0.05), however significantly correlated with forced expiratory volume in 1 s (FEV1) (Ratiolobe: r = 0.61, p main: r = 0.58, p lobe: r = 0.36, p main: r = 0.33, p lobe of smokers without COPD was significantly different from those of moderate COPD and severe or very severe COPD (p main of severe or very severe COPD patients was significantly different from those of other groups (p < 0.05). Conclusions: Quantitative bronchial luminal volumes were reflected the airflow limitation parameters and was corresponded to clinical groups in emphysema patients.

  1. Justification of advisability of using physical factors in the treatment of patients with allergic rhinitis and associated bronchial asthma

    Directory of Open Access Journals (Sweden)

    T. N. Zaripova

    2012-01-01

    Full Text Available The efficiency of treatment of patients with allergic rhinitis and associated bronchial asthma by physical factors and advisability of two-stage treatment are justified. Sixty six patients were observed. The conclusion on the efficiency of treatment was based on the data about the dynamics clinical state of the primary and associated diseases, intensity of inflammation activity in respiratory tract (rhinocytograms, biochemical indices of nasal lavages, level of nitrogen oxide in expired air were studied, patency of airways (manometry, spirography. We compared two versions of treatment: two- and one-stage. Patients of the first group (30 patients received elimination therapy for 3 to 5 days with the following peaty mud application, current of supersonic frequency, massage, inhalations, physical therapy. Patients of the second group (36 patients did not receive elimination therapy. We have found a decrease in intensity of clinical implications of the primary and associated diseases, decrease in inflammation activity, better nasal and bronchial носовой patency, improvement of the mucociliary function in patients of the first group. In the second group, the recovery of the surface epithelium and nasal patency was not observed against the background of improvement of the clinical state.

  2. 间充质干细胞与支气管哮喘%Mesenchymal stem cells and bronchial asthma

    Institute of Scientific and Technical Information of China (English)

    毛雨红; 莫碧文

    2012-01-01

    支气管哮喘是一种气道慢性炎症性疾患,同时也是一种免疫紊乱的变态反应性疾病,其发病机制复杂.间充质干细胞(mesenchymal stem cells,MSCs)是来源于中胚层的具有高度自我更新能力和多向分化潜能的多能干细胞,并且具有强大的免疫调节功能.MSCs这些独特性能将赋予它们在支气管哮喘冶疗中的潜在价值.%Bronchial asthma is a chronic inflammatory airway disease,but also an immune disorder of allergic disease.The pathogenesis of asthma is complex.Mesenchymal stem cells are pluripotent stem cells,derived from the mesoderm,with a highly self-renewal capacity and differentiation potential.These cells also show a great ability to display immunomodulatory capacity.These unique properties of MSCs will be given their potential value in the treatment of bronchial asthma.

  3. 38 CFR 3.380 - Diseases of allergic etiology.

    Science.gov (United States)

    2010-07-01

    ... 38 Pensions, Bonuses, and Veterans' Relief 1 2010-07-01 2010-07-01 false Diseases of allergic... Specific Diseases § 3.380 Diseases of allergic etiology. Diseases of allergic etiology, including bronchial... progress nor as due to the inherent nature of the disease. Seasonal and other acute allergic...

  4. Social networks: a new source of psychological stress or a way to enhance self-esteem? Negative and positive implications in bronchial asthma.

    Science.gov (United States)

    D'Amato, G; Cecchi, L; Liccardi, G; Pellegrino, F; D'Amato, M; Sofia, M

    2012-01-01

    The Internet and, in particular, social networks are an increasingly important part of daily life for both adolescents and adults who maintain a virtual relationship with others sharing interests and goals. Very often, they disclose more about themselves online than they do in person. However, cyberbullying and cyberostracism can be problematic for adolescents and sensitive individuals, who might be negatively affected by social networks. Some studies have shown an increased risk of depression, whereas others suggest beneficial effects through enhanced communication, social connection, and self-esteem. Bronchial asthma is an increasingly frequent disease in the industrialized world, and psychological implications play a role in increasing or in reducing its severity. One year after the case report of an asthma exacerbation that may have been triggered by Facebook, it seems reasonable to analyze the effects of social networks on bronchial asthma.

  5. Social networks: a new source of psychological stress or a way to enhance self-esteem? Negative and positive implications in bronchial asthma.

    Science.gov (United States)

    D'Amato, G; Cecchi, L; Liccardi, G; Pellegrino, F; D'Amato, M; Sofia, M

    2012-01-01

    The Internet and, in particular, social networks are an increasingly important part of daily life for both adolescents and adults who maintain a virtual relationship with others sharing interests and goals. Very often, they disclose more about themselves online than they do in person. However, cyberbullying and cyberostracism can be problematic for adolescents and sensitive individuals, who might be negatively affected by social networks. Some studies have shown an increased risk of depression, whereas others suggest beneficial effects through enhanced communication, social connection, and self-esteem. Bronchial asthma is an increasingly frequent disease in the industrialized world, and psychological implications play a role in increasing or in reducing its severity. One year after the case report of an asthma exacerbation that may have been triggered by Facebook, it seems reasonable to analyze the effects of social networks on bronchial asthma. PMID:23101183

  6. Isolation of elafin and elastase-specific inhibitor (ESI) from bronchial secretions. Evidence of sequence homology and immunological cross-reactivity.

    Science.gov (United States)

    Sallenave, J M; Marsden, M D; Ryle, A P

    1992-01-01

    Evidence is presented that the elastase-specific inhibitor of Mr 2500 (Sallenave, J.-M. & Ryle, A.P. (1991) Biol. Chem. Hoppe-Seyler 372, 13-21) is a biologically active fragment of a larger molecule described in the skin of patients with psoriasis (Wiedow, O., Shroder, J.-M., Gregory, H., Young, J.A. & Christophers, E. (1990) J. Biol. Chem. 265, 14791-14795) which the authors called elafin. We also describe the purification of the complete elafin molecule from bronchial secretions from a patient suffering from bronchial carcinoma, thus showing that the elafin, like the mucus proteinase inhibitor (MPI), is not of single origin but is probably a marker of inflammation (chronic obstructive pulmonary diseases, psoriasis...) present in different tissues. PMID:1536690

  7. Clinical significance of determination of serum IL-2, IL-6 and GM-CSF levels after treatment in pediatric patients with bronchial asthma

    International Nuclear Information System (INIS)

    Objective: To explore the clinical significance of changes of serum IL-2, IL-6 and GM-CSF levels after treatment in pediatric patients with bronchial asthma. Methods: Serum levels of IL-2, IL-6 and GM-CSF were measured with RIA in 36 pediatric patients with bronchiol asthma and 30 controls. Results: Before treatment, the serum levels of IL-6, GM-CSF were significantly higher in the patients than those in controls (P0.05), Serum IL-2 levels were negatively correlated with the IL-6 and GM-CSF levels (r=-0.5846, -0.6018, P<0.01). Conclusion: These cytokines participated in the pathogenesis of bronchial asthma in pediatric patients. Mornitoring the changes of their serum levels was helpful for the management of the diseases. (authors)

  8. Bronchial artery embolization in 'sandwich' pattern for the management of hemoptysis: analysis of clinical effectiveness

    International Nuclear Information System (INIS)

    Objective: To investigate the clinical effectiveness of bronchial artery embolization (BAE) in 'sandwich' pattern for the management of hemoptysis. Methods: During the period from March 2010 to March 2012, a total of 30 patients with hemoptysis were admitted to authors' hospital. BAE in 'sandwich' pattern was carried out in all patients. All patients were followed up for 1-3 years. The recurrence rate and long-term disease control rate were estimated with SPSS version 17.0 software. The BAE method, causes of recurrent hemoptysis and the therapeutic measures were discussed. Results: The immediate hemostasia rate of 'sandwich' pattern BAE was 100% in all patients. Efficiency rate, cure rate and recurrence rate was 30.0% (9/30), 63.3% (19/30) and 6.7% (2/30) respectively. The control rate of hemoptysis was 76% and 68% for 1 year and 2 years respectively. Hemoptysis recurred in two patients during one year following-up period. Angiography showed that in one case an offending vessel remained patent without being embolized (8 days after initial operation), and that in another case the initially embolized left bronchial artery opened again (7 months after initial operation). Hemoptysis did not recur after the two patients underwent the second BAE. Conclusion: For the treatment of massive hemoptysis, the 'sandwich' pattern bronchial artery embolization is safe and effective. Recurrence of hemoptysis may attribute to the missing embolization of the feeding vessels or the formation of collateral circulations. (authors)

  9. Elevated levels of the complement activation product C4d in bronchial fluids for the diagnosis of lung cancer.

    Directory of Open Access Journals (Sweden)

    Daniel Ajona

    Full Text Available Molecular markers in bronchial fluids may contribute to the diagnosis of lung cancer. We previously observed a significant increase of C4d-containing complement degradation fragments in bronchoalveolar lavage (BAL supernatants from lung cancer patients in a cohort of 50 cases and 22 controls (CUN cohort. The present study was designed to determine the diagnostic performance of these complement fragments (hereinafter jointly referred as C4d in bronchial fluids. C4d levels were determined in BAL supernatants from two independent cohorts: the CU cohort (25 cases and 26 controls and the HUVR cohort (60 cases and 98 controls. A series of spontaneous sputum samples from 68 patients with lung cancer and 10 controls was also used (LCCCIO cohort. Total protein content, complement C4, complement C5a, and CYFRA 21-1 were also measured in all cohorts. C4d levels were significantly increased in BAL samples from lung cancer patients. The area under the ROC curve was 0.82 (95%CI = 0.71-0.94 and 0.67 (95%CI = 0.58-0.76 for the CU and HUVR cohorts, respectively. In addition, unlike the other markers, C4d levels in BAL samples were highly consistent across the CUN, CU and HUVR cohorts. Interestingly, C4d test markedly increased the sensitivity of bronchoscopy in the two cohorts in which cytological data were available (CUN and HUVR cohorts. Finally, in the LCCCIO cohort, C4d levels were higher in sputum supernatants from patients with lung cancer (area under the ROC curve: 0.7; 95%CI = 0.56-0.83. In conclusion, C4d is consistently elevated in bronchial fluids from lung cancer patients and may be used to improve the diagnosis of the disease.

  10. Elevated levels of the complement activation product C4d in bronchial fluids for the diagnosis of lung cancer.

    Science.gov (United States)

    Ajona, Daniel; Razquin, Cristina; Pastor, Maria Dolores; Pajares, Maria Jose; Garcia, Javier; Cardenal, Felipe; Fleischhacker, Michael; Lozano, Maria Dolores; Zulueta, Javier J; Schmidt, Bernd; Nadal, Ernest; Paz-Ares, Luis; Montuenga, Luis M; Pio, Ruben

    2015-01-01

    Molecular markers in bronchial fluids may contribute to the diagnosis of lung cancer. We previously observed a significant increase of C4d-containing complement degradation fragments in bronchoalveolar lavage (BAL) supernatants from lung cancer patients in a cohort of 50 cases and 22 controls (CUN cohort). The present study was designed to determine the diagnostic performance of these complement fragments (hereinafter jointly referred as C4d) in bronchial fluids. C4d levels were determined in BAL supernatants from two independent cohorts: the CU cohort (25 cases and 26 controls) and the HUVR cohort (60 cases and 98 controls). A series of spontaneous sputum samples from 68 patients with lung cancer and 10 controls was also used (LCCCIO cohort). Total protein content, complement C4, complement C5a, and CYFRA 21-1 were also measured in all cohorts. C4d levels were significantly increased in BAL samples from lung cancer patients. The area under the ROC curve was 0.82 (95%CI = 0.71-0.94) and 0.67 (95%CI = 0.58-0.76) for the CU and HUVR cohorts, respectively. In addition, unlike the other markers, C4d levels in BAL samples were highly consistent across the CUN, CU and HUVR cohorts. Interestingly, C4d test markedly increased the sensitivity of bronchoscopy in the two cohorts in which cytological data were available (CUN and HUVR cohorts). Finally, in the LCCCIO cohort, C4d levels were higher in sputum supernatants from patients with lung cancer (area under the ROC curve: 0.7; 95%CI = 0.56-0.83). In conclusion, C4d is consistently elevated in bronchial fluids from lung cancer patients and may be used to improve the diagnosis of the disease. PMID:25799154

  11. Gastro-bronchial fistula after laparoscopic nissen fundoplication.

    Science.gov (United States)

    Faraj, Walid; Khalifeh, Mohamad; Soweid, Asaad; Habli, Nader; Selmo, Francesca; Priest, Oliver; Jamali, Faek R

    2007-10-01

    Gastro-bronchial fistula (GBF) is an unusual complication of Nissen fundoplication, particularly when performed via a transabdominal approach. The mechanism of such fistula is thought to be related to a previously untreated ulcer in the mucosa of the wall of the gastric wrap or to a subclinical injury to the gastric wall during the process of division of the short gastric vessels and gastric mobilization. This process is greatly aided by herniation of the wrap into the chest in the postoperative period, placing the stomach in intimate contact with the bronchial tree. The diagnosis of GBF is often difficult to establish and requires a high index of suspicion. Most investigative studies tend to be unrevealing. Historically, an upper gastrointestinal series was the recommended study of choice in the literature. Newer reports, however, highlight the value and importance of upper endoscopy as a diagnostic tool in this condition. The majority of GBF were reported in the era of transthoracic Nissen fundoplication. The incidence of this complication seems to have markedly decreased after the widespread adoption of the transabdominal approach to the treatment of GERD. We are reporting the second case in the literature of a GBF developing after a laparoscopic Nissen fundoplication in a 28-year-old male patient. This case report should serve to increase awareness of this uncommon complication that should be considered in the differential diagnosis of patients with recurrent pneumonia or refractory hemoptysis after major upper gastrointestinal surgical procedures.

  12. Preoperative Assessment of Different Treatment Modalities in Bronchial Asthma Patients

    Directory of Open Access Journals (Sweden)

    Kawther A. Azzam M.D. and **Sahar S. Khattab MD

    2003-06-01

    Full Text Available This study was performed to assess the effectiveness of acupuncture and medical therapies of bronchial asthma preoperatively. Sixty patients suffered from mild to moderate bronchial asthma and coming for elective operations were chozen from the outpatient clinic of Al-Zahraa University Hospital. Patients were randomly divided into three equal groups (n=20 each. Group I patients (drug group received oral theophylline and Salbutamol (ventolin inhaler according to the needs. Group II patients (drug +ear acupuncture group received same medical treatment as in group I and added ear acupuncture. Group III patients (drug + ear and body acupuncture group received same medical treatment as in group I and II and added ear and body acupuncture. Ventilatory function tests through spirometer and interleukin-13 estimation were performed before treatment and after two weeks of treatment. Improvement of subjective and objective parameters had occurred with significant decrease in the mean serum level of interleukin-13 and decrease in the mean number of using b-agonist puffs after two weeks of treatment in the three groups, with the best results being in group III than in group II and then in group I. Conclusion: Interleukin-13 estimation togheter with ventilatory function tests is a useful parameter for pre-operative assessment and evaluation of asthmatic patients. Also medication was significantly reduced when combined with acupuncture.

  13. Estradiol increases mucus synthesis in bronchial epithelial cells.

    Directory of Open Access Journals (Sweden)

    Anthony Tam

    Full Text Available Airway epithelial mucus hypersecretion and mucus plugging are prominent pathologic features of chronic inflammatory conditions of the airway (e.g. asthma and cystic fibrosis and in most of these conditions, women have worse prognosis compared with male patients. We thus investigated the effects of estradiol on mucus expression in primary normal human bronchial epithelial cells from female donors grown at an air liquid interface (ALI. Treatment with estradiol in physiological ranges for 2 weeks caused a concentration-dependent increase in the number of PAS-positive cells (confirmed to be goblet cells by MUC5AC immunostaining in ALI cultures, and this action was attenuated by estrogen receptor beta (ER-β antagonist. Protein microarray data showed that nuclear factor of activated T-cell (NFAT in the nuclear fraction of NHBE cells was increased with estradiol treatment. Estradiol increased NFATc1 mRNA and protein in ALI cultures. In a human airway epithelial (1HAE0 cell line, NFATc1 was required for the regulation of MUC5AC mRNA and protein. Estradiol also induced post-translational modification of mucins by increasing total fucose residues and fucosyltransferase (FUT-4, -5, -6 mRNA expression. Together, these data indicate a novel mechanism by which estradiol increases mucus synthesis in the human bronchial epithelium.

  14. Chinese and Uighur medicine diagnostic criteria of the evaluation of the Modern drug treatment side-effects in bronchial asthma patients

    Institute of Scientific and Technical Information of China (English)

    Dubrovin Denis; Igor Gogol; Mikhail Baranov; Nurmuhammat Amat; Halmurat Upur

    2014-01-01

    We studied Chinese and Uighur medicines and create an automated computer diagnostics system according to principals of Uighur medicine for evaluation of bronchial asthma patient′s state .498 patients with bronchial asthma were enrolled the automated computer diagnostic program .304 patients were evalu‐ated in the process of drug and non‐drug treatment .Savda asthma type of Uighur medicine do not corre‐sponds with any of the defined clinico‐pathogenetic variants of the disease .Thus ,prevailing of atopic bron‐chial asthma with probability of 0 .7 is defined with Savda categories with such as ‘Lungs energy deficien‐cy’ ,‘Spleen energy deficiency’ and ‘Kidneys Yin deficiency’ (P <0 .01) variants in Chinese Medicine . The combination of infectious‐dependent variant of bronchial asthma Abnormal Savda syndrome with atopy with a probability of 0 .8 is accompanied by the categories of ‘Hot and Full’ asthma with such variants as‘Lungs energy deficiency’ ‘Spleen energy deficiency’ ,‘Kidneys Yin deficiency’ (P < 0 .01) .Patients with an easier case of Abnormal Savda syndrome bronchial asthma in 71% of cases have prevailing diagnos‐tic categories of ‘External ,Full and Cold’ asthma .Patients with a harder case of Abnormal Savda in 74%of cases belongs to the categories of ‘Internal ,Empty and Hot’ asthma .So ,hormone dependency of the disease in 69% of cases is accompanied by the ‘Kidneys Yin deficiency’ variant and ,if there is an aspirin component in the pathogenesis of mixed asthma ,in 83% of cases ‘Kidneys Yin deficiency’ and ‘Kidneys Yang deficiency’ variants are defined .

  15. 小儿支气管哮喘的护理%Nursing Experience of Bronchial Asthma in Children

    Institute of Scientific and Technical Information of China (English)

    邹琳

    2014-01-01

    Objective To investigate the ef ect of nursing intervention on therapeutic ef ect in children with bronchial asthma and the ef ect of the implementation of health education. Methods In our hospital from 2008 July to 2009 June were treated 84 cases of bronchial, properly care;and 84 cases of children with asthma in children and their parents on the knowledge base of bronchial asthma, targeted to the guide. Results 98.81% children improvement of clinical manifestations, parents can bet er with treatment and prevention, reduce asthma at acks, to improve the quality of life. Conclusion The nursing and health education of good, can make the bronchial asthma children get bet er treatment, shorten the course of disease, accelerate rehabilitation.%目的探讨护理干预在支气管哮喘患儿和健康教育的实施效果的孩子对疗效的影响。方法在我们医院从2013年1月~2014年1月收治的84例支气管哮喘的治疗是合理的,适当的照顾;与孩子们和他们的父母哮喘支气管哮喘患儿84例的知识基础,有针对性的指导。结果98.81%患儿临床表现均有明显改善,肺部哮鸣音消失或减轻,咳嗽,胸闷,呼吸急促的显著降低;通过健康教育,儿童,家长可以有更好的治疗与预防,减少哮喘发作,改善生活质量。结论好的护理和健康教育,可使支气管哮喘儿童得到更好的治疗,缩短病程,加快康复。

  16. PKC activation induces inflammatory response and cell death in human bronchial epithelial cells.

    Directory of Open Access Journals (Sweden)

    Hyunhee Kim

    Full Text Available A variety of airborne pathogens can induce inflammatory responses in airway epithelial cells, which is a crucial component of host defence. However, excessive inflammatory responses and chronic inflammation also contribute to different diseases of the respiratory system. We hypothesized that the activation of protein kinase C (PKC is one of the essential mechanisms of inflammatory response in airway epithelial cells. In the present study, we stimulated human bronchial lung epithelial (BEAS-2B cells with the phorbol ester Phorbol 12, 13-dibutyrate (PDBu, and examined gene expression profile using microarrays. Microarray analysis suggests that PKC activation induced dramatic changes in gene expression related to multiple cellular functions. The top two interaction networks generated from these changes were centered on NFκB and TNF-α, which are two commonly known pathways for cell death and inflammation. Subsequent tests confirmed the decrease in cell viability and an increase in the production of various cytokines. Interestingly, each of the increased cytokines was differentially regulated at mRNA and/or protein levels by different sub-classes of PKC isozymes. We conclude that pathological cell death and cytokine production in airway epithelial cells in various situations may be mediated through PKC related signaling pathways. These findings suggest that PKCs can be new targets for treatment of lung diseases.

  17. Secondhand smoke inhibits both Cl- and K+ conductances in normal human bronchial epithelial cells

    Directory of Open Access Journals (Sweden)

    Cohen Noam A

    2009-11-01

    Full Text Available Abstract Secondhand smoke (SHS exposure is an independent risk factor for asthma, rhinosinusitis, and more severe respiratory tract infections in children and adults. Impaired mucociliary clearance with subsequent mucus retention contributes to the pathophysiology of each of these diseases, suggesting that altered epithelial salt and water transport may play an etiological role. To test the hypothesis that SHS would alter epithelial ion transport, we designed a system for in vitro exposure of mature, well-differentiated human bronchial epithelial cells to SHS. We show that SHS exposure inhibits cAMP-stimulated, bumetanide-sensitive anion secretion by 25 to 40% in a time-dependent fashion in these cells. Increasing the amount of carbon monoxide to 100 ppm from 5 ppm did not increase the amount of inhibition, and filtering SHS reduced inhibition significantly. It was determined that SHS inhibited cAMP-dependent apical membrane chloride conductance by 25% and Ba2+-sensitive basolateral membrane potassium conductance by 50%. These data confirm previous findings that cigarette smoke inhibits chloride secretion in a novel model of smoke exposure designed to mimic SHS exposure. They also extend previous findings to demonstrate an effect on basolateral K+ conductance. Therefore, pharmacological agents that increase either apical membrane chloride conductance or basolateral membrane potassium conductance might be of therapeutic benefit in patients with diseases related to SHS exposure.

  18. COMPARATIVE EFFICACY OF INHALED DRUGS FORMOTEROL / BECLOMETHASONE AND FORMOTEROL / BUDESONIDE AND BRONCHIAL ASTHMA

    Directory of Open Access Journals (Sweden)

    V. A. Nikitin

    2016-01-01

    Full Text Available There were examined 86 patients aged 20 to 65 years with a diagnosis of bronchial asthma (BA. The objective is to evaluate the level of disease control, to study the effectiveness, safety and usability when used Foster (beclomethasone dipropionate and formoterol fumarate fixed combination Combination and Foradil (formoterol fumarate and budesonide in free combination. Patients were divided into 2 groups randomly. The first group received Foster (beclomethasone dipropionate and formoterol fumarate in a fixed combination, and the second group — Foradil Combi (budesonide and formoterol fumarate in a free combination. Results of the study were assessed for 11-13 days and 3 months. The treatment showed a significant reduction in the severity of clinical symptoms in groups 1 and 2. Respiratory function after 3 months in group 1 improved to 17,4 ± 3,2% (p <0.05, and the second at 6,0 ± 3,3% (p> 0.1. Full control of the disease was achieved in one group, 48.83% of patients, and the second at 44.19% of the patients.

  19. Type I IL-1 Receptor (IL-1RI as Potential New Therapeutic Target for Bronchial Asthma

    Directory of Open Access Journals (Sweden)

    Jyh-Hong Lee

    2010-01-01

    Full Text Available The IL-1R/TLR family has been receiving considerable attention as potential regulators of inflammation through their ability to act as either activators or suppressors of inflammation. Asthma is a chronic inflammatory disease characterized by airway hyperresponsiveness, allergic inflammation, elevated serum total, allergen-specific IgE levels, and increased Th2 cytokine production. The discovery that the IL-1RI–IL-1 and ST2–IL-33 pathways are crucial for allergic inflammation has raised interest in these receptors as potential targets for developing new therapeutic strategies for bronchial asthma. This paper discusses the current use of neutralizing mAb or soluble receptor constructs to deplete cytokines, the use of neutralizing mAb or recombinant receptor antagonists to block cytokine receptors, and gene therapy from experimental studies in asthma. Targeting IL-1RI–IL-1 as well as ST2–IL-33 pathways may promise a disease-modifying approach in the future.

  20. A CROSS SECTIONAL STUDY OF BRONCHIAL ASTHMA IN ALLERGIC RHINITIS PATIENTS: A COMMUNITY BASED ASSESSMENTS AMONG ADULTS IN BANGALORE

    Directory of Open Access Journals (Sweden)

    Giriraja

    2015-12-01

    Full Text Available BACKGROUND Allergic rhinitis is rather erroneously viewed as a trivial disease, it is important to note that it can significantly affect the quality of life. There is significant overlap between bronchial asthma and allergic rhinitis. AIMS This study was done to assess the prevalence of asthmatics in allergic rhinitis patients. MATERIALS AND METHODS A community based cross sectional study was conducted in Bangalore, among 1000 adults aged 30 yrs and above. Allergic rhinitis was diagnosed as per ARIA guidelines. Sperometery was done to diagnose asthma among them. Multivariate logistic regression analysis has been used to find the association of risk factors with disease. RESULTS Among subjects with allergic rhinitis, 40(33% were also diagnosed to be suffering from concomitant asthma showing a considerable overlap between rhinitis and asthma. CONCLUSION Burden of allergic rhinitis is high with a considerable overlap with asthma. This highlights the importance of early and regular treatment

  1. PEF variability, bronchial responsiveness and their relation to allergy markers in a random population (20-70 yr).

    Science.gov (United States)

    Boezen, H M; Postma, D S; Schouten, J P; Kerstjens, H A; Rijcken, B

    1996-07-01

    We investigated the coherence of bronchial hyperresponsiveness (BHR) and peak expiratory flow (PEF) variability in their relation to allergy markers and respiratory symptoms in 399 subjects (20-70 yr). Bronchial hyperresponsiveness to methacholine was defined by both the provocative dose causing a fall in FEV1 of 20%, and the dose-response slope. PEF variability was determined as diurnal PEF variation (amplitude percent mean) and between-day PEF variation. Skin tests positivity, serum total IgE, and specific IgE (RAST) for house-dust mite (HDM), cat, timothy grass, and birch ("pollen") were determined, as well as the number of peripheral blood eosinophils. Wheeze and nocturnal dyspnea were defined as asthma-like symptoms; dyspnea > or = grade 3, cough and phlegm as chronic obstructive pulmonary disease (COPD)-like symptoms. The reciprocal of the dose-response slope and PEF variability were significantly correlated (r = -0.39). Subjects with a positive skin test for HDM (odds ratio [OR] = 3.9), cat (OR = 8.3), or pollen (OR = 3.6), or specific IgE for HDM (OR = 2.3), cat (OR = 3.4), or pollen (OR = 1.9) had increased risk of BHR compared with the reference group (all p values blood eosinophils. There are different associations of BHR and PEF variability with allergy markers. Although BHR and PEF variability are significantly correlated, they cannot be used interchangeably in epidemiologic settings. PMID:8680695

  2. Transarterial microcatheter glue embolization of the bronchial artery for life-threatening hemoptysis: Technical and clinical results

    Energy Technology Data Exchange (ETDEWEB)

    Baltacioglu, Feyyaz [Marmara University School of Medicine, Department of Radiology, Istanbul (Turkey)], E-mail: fbaltaci@yahoo.com; Cimsit, Nuri Cagatay [Marmara University School of Medicine, Department of Radiology, Istanbul (Turkey)], E-mail: cagataycimsit@gmail.com; Bostanci, Korkut [Marmara University School of Medicine, Department of Thoracic Surgery, Istanbul (Turkey)], E-mail: kbostanci@marmara.edu.tr; Yueksel, Mustafa [Marmara University School of Medicine, Department of Thoracic Surgery, Istanbul (Turkey)], E-mail: myuksel@marmara.edu.tr; Kodalli, Nihat [Marmara University School of Medicine, Department of Radiology, Istanbul (Turkey)], E-mail: nihatkodalli@yahoo.com

    2010-02-15

    Objectives: To report our experience with transarterial glue embolization of the bronchial artery for life-threatening hemoptysis. Materials and methods: Twenty-five patients underwent bronchial artery embolization, using coaxial microcatheter technique, with a liquid agent, n-butyl-2-cyanoacrylate (NBCA), named glue, for life-threatening hemoptysis. The technical and clinical outcomes were followed in terms of immediate control of bleeding, recurrence of hemoptysis and complications of the procedure. Results: Four patients had acute hemoptysis when they were evaluated. The average number of arteries embolized per patient was 2.9. BAEs were successful in controlling hemoptysis immediately in all 25 patients (100%) and in 24 patients (96%) at 1 month follow-ups. One patient had recurrent hemoptysis on the tenth day after embolization. The follow-up time ranged from 2 to 63 months (mean 14 months). Six patients (25%) died all as a result of their disease process. Bleeding recurred in 3 patients after 30 days (7th, 11th, 12th months). One patient had vomitting attacks with dysphagia after the procedure that lasted 24 h. Three patients had transient thoracic pain lasting 3-5 days. There were no procedure related spinal or vascular complications. Conclusions: Glue embolization with microcatheter technique is a safe and effective treatment in cases of life-threatening hemoptysis with a very high rate of success and low rate of complications.

  3. Comparison of stayers, dropouts, and newcomers in a longitudinal population study of asthma and bronchial hyperresponsiveness: introduction of bias?

    Science.gov (United States)

    Ulrik, C S

    1995-01-01

    A random sample of children and adolescents from the general population in Copenhagen, Denmark, has been examined twice (6 years apart) with respect to asthma, allergy, and nonspecific bronchial hyperresponsiveness. To investigate potential bias resulting from loss of baseline subjects at follow-up (dropout bias) and bias resulting from subjects entering the study at the follow-up stage (newcomer bias), stayers (subjects who participated in both examinations) were compared to dropouts and newcomers, respectively. The sample consisted of 983 subjects (aged 7-17 years at the time of the baseline study), of whom 408 (199 boys) participated in both examinations (stayers), 119 (62 boys) in the baseline study only (dropouts), and 257 (124 boys in the follow-up study only (newcomers). Thus, a total of 784 subjects (80% of the sample) were examined either once or twice. At baseline dropouts did not differ from stayers with respect to anthropometric data, smoking habits, pulmonary function, or prevalence of positive skin prick tests, bronchial hyperresponsiveness, asthma, and allergic diseases. Likewise, these variables for newcomers were not different from those of the stayers apart from a significantly higher smoking rate in newcomers (45% vs. 32%, p = 0.003). Based on these findings and the high overall response rate, it seems reasonable to assume that the group of stayers is representative of the whole sample, apart from an underestimation of the number of smokers and, therefore, an underestimation of the risks associated with smoking.(ABSTRACT TRUNCATED AT 250 WORDS)

  4. A normal and biotransforming model of the human bronchial epithelium for the toxicity testing of aerosols and solubilised substances.

    Science.gov (United States)

    Prytherch, Zoë C; BéruBé, Kelly A

    2014-12-01

    In this article, we provide an overview of the experimental workflow by the Lung and Particle Research Group at Cardiff University, that led to the development of the two in vitro lung models - the normal human bronchial epithelium (NHBE) model and the lung-liver model, Metabo-Lung™. This work was jointly awarded the 2013 Lush Science Prize. The NHBE model is a three-dimensional, in vitro, human tissue-based model of the normal human bronchial epithelium, and Metabo-Lung involves the co-culture of the NHBE model with primary human hepatocytes, thus permitting the biotransformation of inhaled toxicants in an in vivo-like manner. Both models can be used as alternative test systems that could replace the use of animals in research and development for safety and toxicity testing in a variety of industries (e.g. the pharmaceutical, environmental, cosmetics, and food industries). Metabo-Lung itself is a unique tool for the in vitro detection of toxins produced by reactive metabolites. This 21st century animal replacement model could yield representative in vitro predictions for in vivo toxicity. This advancement in in vitro toxicology relies on filter-well technology that will enable a wide-spectrum of researchers to create viable and economic alternatives for respiratory safety assessment and disease-focused research. PMID:25635646

  5. To Explore the Treatment of Bronchial Asthma in Children%浅析小儿支气管哮喘的治疗

    Institute of Scientific and Technical Information of China (English)

    杨秀珍

    2015-01-01

    Objective To treat children with bronchial asthma and discuss the effect.MethodsSelected 30 cases of child patients treated in our hospital from January 2012 to December 2014 were researched and analyzed. Results22 cases were cured, four patients improved , and the remaining 2 patients invalid, so, improvement of this disease was 93.3 %. Conclusion If getting timely and effective treatment , children with bronchial asthma are generally able to get better results , while the treatment of bronchial asthma has an important role in adults.%目的:对小儿支气管哮喘病的治疗及效果进行探讨。方法随机选取我院2012年1月~2014年12月间收治的本病患儿30例进行研究分析。结果22例患者痊愈,有5例患者有所好转,其余3例患者治疗无效,统计得知,本病的好转率为93.3%。结论小儿支气管哮喘若能进行及时有效的治疗,一般都能够得到较好的疗效,同时对成人支气管哮喘的治疗有重要作用。

  6. Randomized controlled study of CBT in bronchial asthma

    Directory of Open Access Journals (Sweden)

    Grover Naveen

    2007-01-01

    Full Text Available The aim of the present study was to find out efficacy of cognitive behavior therapy, as an adjunct to standard pharmacotherapy, in bronchial asthma. In a random-ized two-group design with pre-and post assessments, forty asthma patients were randomly allotted to two groups: self management group and cognitive behavior therapy group. Both groups were exposed to 6-8 weeks of intervention, asthma self management program and cognitive behavior therapy. Assessment measures used were-Semi structured interview schedule, Asthma Symptom Checklist, Asthma di-ary, Asthma Bother Profile, Hospital Anxiety & Depression Scale, AQLQ and Peak Expiratory Flow Rate. Within group comparison showed significant improvement in both groups at the post assessment. Between group comparisons showed that CBT group reported significantly greater change than that of SM group. Cognitive behavior therapy helps in improving the managment of asthma.

  7. [Efficiency of kinesi- and hydrokinesitherapy in children with bronchial asthma].

    Science.gov (United States)

    Surovenko, T N; Iashchuk, A V; Iansons, T Ia; Ezhov, S N

    2003-01-01

    The authors review efficiency of various programs of kinesi and hydrokinesitherapy of children with atopic bronchial asthma (BA). Efficiency of the treatment was assessed by quality of life using the questionnaire by A. West, D. French "Childhood asthma questionnaire" (adapted for Russia by V. I. Petrov et al). Monitoring of the activity of allergic inflammation of the upper respiratory tracts was performed by examination of the nasal lavage fluid for nitric oxide metabolites, of the lower respiratory tracts--by the metabolites in the condensate of the expired air. It is shown that hydrokinesitherapy raises BA children's quality of life and declines inflammation activity leading to reduction of the number of BA exacerbations and hospitalizations. The above criteria of the treatment efficacy proved sensitive.

  8. Contribution of CT for the diagnosis of bronchial carcinoids

    Energy Technology Data Exchange (ETDEWEB)

    Koenig, R.; Kaick, G. van; Toomes, H.

    1984-03-01

    The CT findings in 10 patients with carcinoid tumors of the lung are reported. The tumors were located in the hilar region, in the perihilar region and in the periphery of the lung. Dystelectasis and atelectasis of the lung with poststenotic inflammation were found in 4 patients. Infiltrating tumor growth with lymph node metastases were detected only once. This tumor was not able to be differentiated from other malignant space occupying lesions. There were no reliable CT criteria for bronchial carcinoids. Compared to conventional radiography the CT examination has the following advantages: better demonstration of size and location of the tumor, and the exlusion of infiltrating tumor growth, enlarged lymph nodes and calcified lung nodules.

  9. Ventilation and perfusion scintiscanning in unresectable bronchial carcinomas under radiotherapy

    International Nuclear Information System (INIS)

    Ventilation and perfusion scintiscanning using Kr-81m-gas and Tc-99m microspheres in addition to X-ray thorax control examination were performed in patients with inoperable bronchoscopically confirmed bronchial carcinoma prior to radiotherapy, during irradiation after 30 Gy and at the scheduled end of treatment. Compared with the static-morphological X-ray picture, lung scintigraphy provides additional information about regional lung functions. Perfusion scintiscanning shows to be more sensitive and more accurate a method than ventilation scintiscanning. In follow-up under radiotherapy, valuable suggestions concerning determination of target volume and final dose are supplied when aiming at a palliative objective. Early detection and confirmation of pulmonary metastases or of a lymphangiosis carcinomatosa allow to revise an originally curative objective. (orig.)

  10. Recurrent acute kidney injury associated with metastatic bronchial carcinoid.

    Science.gov (United States)

    Barton, James C; Barton, J Clayborn; Bertoli, Luigi F

    2012-01-01

    Acute kidney injury (AKI) is a rare complication of carcinoid syndrome. A 61-year-old man developed carcinoid syndrome 51 months after pneumonectomy for bronchial carcinoid, and 8 episodes of AKI 101 to 118 months after pneumonectomy. Serum chromogranin A and urine 5-hydroxyindoleacetic acid levels were elevated for more than 1 year before AKI occurred. Each episode was characterized by flushing, facial edema, mild diarrhea, necrosis of hepatic metastatic nodules, mild oliguria, hyponatremia, acidosis, hypokalemia, hypomagnesemia and hyperphosphatemia. He did not have elevated urine sodium levels or osmolality, hypotension or hypertension. Plasma levels of dopamine, epinephrine and norepinephrine, measured during a single episode, were markedly elevated. Serum creatinine levels returned to normal after most episodes. Hyponatremia persisted but was more severe during AKI. Elevated plasma levels of vasoactive substances other than 5-hydroxytryptamine, perhaps dopamine or other catecholamines, could explain recurrent AKI. The natriuretic effect of elevated plasma dopamine levels could explain chronic hyponatremia. PMID:22008780

  11. Bronchial thermoplasty: reappraising the evidence (or lack thereof).

    Science.gov (United States)

    Iyer, Vivek N; Lim, Kaiser G

    2014-07-01

    Bronchial thermoplasty (BT) involves the application of radiofrequency energy to visible proximal airways to selectively ablate airway smooth muscle. BT is the first nonpharmacologic interventional therapy approved by the US Food and Drug Administration (FDA) for severe asthma. This approval was based on the results of the pivotal Asthma Intervention Research (AIR)-2 trial, which is the only randomized, double-blind, sham-controlled trial of BT. The primary end point of the AIR-2 trial was improvement in the Asthma Quality of Life Questionnaire (AQLQ). The results of the AIR-2 trial have generated enormous interest, controversy, and confusion regarding the true efficacy of BT for severe asthma. Current marketing of BT highlights its use for patients with "severe" asthma, which is interpreted by most practicing clinicians as meaning oral corticosteroid dependence, frequent exacerbations, or a significantly reduced FEV1 with a poor quality of life. Did the AIR-2 trial include patients with a low FEV1, oral steroid dependence, or frequent exacerbations? Did the trial show efficacy for any of the primary or secondary end points? The FDA approved the device based on the reduction in severe asthma exacerbations. However, were the rates of asthma exacerbations, ED visits, or hospitalizations truly different between the two groups, and was this type of analysis even justified given the original study design? This commentary is designed to specifically answer these questions and help the practicing clinician navigate the thermoplasty literature with confidence and clarity. We carefully dissect the design, conduct, and results of the AIR-2 trial and raise serious questions about the efficacy of bronchial thermoplasty.

  12. Bronchial stump closure with amniotic membrane in animal model

    Directory of Open Access Journals (Sweden)

    Gholamreza Mohajeri

    2014-01-01

    Full Text Available Background: Coverage of the bronchial stumps (BSs with adjacent tissues can improve healing and reduce bronchial complications in complex thoracic surgery. There is no evidence for the application of human amnion allograft for prevention of air leak from the BS. The comparison of the amniotic membrane (AM and pleural patch for BS healing after lobectomy in dogs was our aim in this study. Materials and Methods: A total of eight males and females 12-24-month-old dogs between 17 and 22 kg body-weight were used in this study in 2010, Isfahan University of Medical Sciences. Animals were separated into two groups: group A (n = 4; amniotic membrane and group P (n = 4; pleural patch according to the BS closure technique performed. After lobectomy of the right middle lobe, the BS was closed, while a small bronchopleural fistula (BPF was created by inserting a catheter via edges of closed stump. Then, it was covered with a piece of AM3 × 3 cm in group A and with a pedicle graft of pleura in group P. Rethoracotomy was performed after 15 days of observation, and the BS was removed for histological examination. Histological healing was classified as complete or incomplete healing. Neoangiogenesis was measured by Von Willebrand expression using immunohistochemistry (IHC. Data were analyzed by SPSS version 15 using Fisher′s exact test, Mann-Whitney test, and T tests. Results: BPF complications were not seen during observation period. There was no significant difference in histological healing between two groups. Similarly, no significant difference was observed between the groups in terms of neoangiogenesis based on IHC examination (P value = 0.69. Conclusion: Human amnion allograft could be as effective as pleural patch for BS wrapping following pulmonary resections.

  13. Pranlukast hydrate in the treatment of pediatric bronchial asthma

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    Yoshihara S

    2013-07-01

    Full Text Available Shigemi Yoshihara Department of Pediatrics, Dokkyo Medical University, Tochigi, Japan Abstract: Pranlukast hydrate is a potent, selective, orally active, cysteinyl leukotriene antagonist that binds at the type 1 receptor. It is used as a 10% dry syrup to treat asthma and allergic rhinitis in pediatric patients. In a 4-week, dose-finding study, a dose-dependent improvement in lung function was observed in pediatric patients with asthma at an optimal dose of 5.1–10 mg/kg/day. In a comparative, randomized, double-blind, 4-week multicenter trial, pranlukast dry syrup 7 mg/kg/day achieved significantly better final overall improvement (71.4% versus oxatomide 1 mg/kg/day (37.2% in pediatric patients older than one year with asthma. In two 12-week, open-label trials and in a long-term open-label trial of treatment for up to 24 months, pranlukast dry syrup improved asthma control over baseline values. In a prospective post-marketing surveillance study and a long-term follow-up study, the safety and efficacy of pranlukast dry syrup was confirmed in infants younger than one year with bronchial asthma. In a 4-week, open-label trial, pranlukast dry syrup improved overall health-related quality of life and physical and emotional domain scores over baseline in pediatric patients with asthma. In a randomized, multicenter, double-blind, placebo-controlled, two-period crossover trial, pranlukast dry syrup significantly inhibited exercise-induced bronchospasm in children with asthma compared with placebo. In a modified Childhood Asthma Control Test, pranlukast dry syrup was significantly more effective in controlling asthma in patients younger than 4 years with the common cold. These findings show that pranlukast is useful and beneficial for treating pediatric patients with bronchial asthma. Keywords: pranlukast, leukotriene receptor antagonist, pediatric asthma

  14. Peak expiratory flow variability, bronchial responsiveness, and susceptibility to ambient air pollution in adults

    NARCIS (Netherlands)

    Boezen, M; Schouten, Jan; Rijcken, B; Vonk, J; Gerritsen, J; Hoek, G; Brunekreef, B; Postma, D

    1998-01-01

    Bronchial hyperresponsiveness (BHR) and peak expiratory flow (PEF) variability are associated expressions of airway lability, yet probably reflect different underlying pathophysiologic mechanisms. We investigated whether both measures can be used interchangeably to identify subjects who are suscepti

  15. Influence of the tobacco smoking on the bronchial asthma in teenagers

    Directory of Open Access Journals (Sweden)

    B. Ts. Batozhargalova

    2012-01-01

    Full Text Available Comparative epidemiological research on studying prevalence tobacco smoking and respiratory symptoms among rural teenagers with bronchial asthma. Control group have made non-smoking (n = 59, and skilled — smoking teenagers (n = 72. Prevalence of smoking among adolescents with bronchial asthma has made 55% against 44,3% on the average at adolescents (p < 0.05. At smokers sick of a bronchial asthma the presence of respiratory complaints, level of СO in exhaled air and urinary cotinine has been raised. Authentic communication of smoking with a series of symptoms bronchial asthma and chronic bronchitis in teenagers, shown is taped by a high and average parity of chances.

  16. Origin of bronchial and pulmonary elastic fibers and their role in some pathological events.

    Science.gov (United States)

    Gabbrielli, S; Stanflin, N; Di Preta, F

    1994-06-01

    The purpose of this contribution is to place a higher value on the existence of a turn-over of bronchial and pulmonary elastic fibers: the former would derive from the smooth muscle cells of the bronchial wall, the latter from the interstitial myofibroblasts of the alveolar septa. The reduction of the number of the elastic fibers in the bronchial wall during chronic atrophic bronchitis depend on the atrophy of the bronchial smooth muscle cells; in the alveolar septa the hyperplasia of the elastic fibers during pulmonary fibroses depend on the myofibroblastic proliferation, while the reduction of the elastic fibers number during pulmonary enphysema depend on the reduction in the number of myofibroblasts. PMID:7808795

  17. Spectral Karyotyping Detects Chromosome Damage in Bronchial Cells of Smokers and Patients with Cancer

    OpenAIRE

    Varella-Garcia, Marileila; Chen, Lin; Powell, Roger L.; Hirsch, Fred R.; Kennedy, Timothy C.; Keith, Robert; Miller, York E.; Mitchell, John D; Franklin, Wilbur A.

    2007-01-01

    Rationale: Lung cancer is a multistep process that is preceded and often accompanied by molecular cytogenetic lesions in benign bronchial epithelium, the precise character, extent and timing of which are not well defined.

  18. [Incidentally detected bronchial artery aneurysm with combined operation for mitral regurgitation;report of a case].

    Science.gov (United States)

    Sato, Hisashi; Oteki, Hitoshi; Naito, Kozo; Yunoki, Junji

    2015-02-01

    A 77-year-old woman was admitted to the hospital for heart failure with orthopnea. Echocardiography revealed massive mitral regurgitation. During preoperative cardiac catheterization, an aneurysm was indentified incidentally just below the tracheal carina. Three dimensional computed tomography showed three bronchial artery aneurysms behind the pulmonary artery and the left atrium. The proximal aneurysm was the largest and was 22 mm in diameter. It was resected by retracting the ascending aorta to the left, the superior vana cava to the right and right pulmonary artery cranially under cardiopulmonary bypass, and mitral valve plasty was performed. We believed that resection of the proximal aneurysm would cause thrombotic occlusion of the other 2 aneurysms. Bronchial artery aneurysm is a rare entity that is observed in fewer than 1% of those who undergo selective bronchial arteriography. In addition, because bronchial artery aneurysm is a potentially life-threatening lesion, it should be treated promptly when diagnosed.

  19. “Peripheral Neuropathy Crippling Bronchial Asthma”: Two Rare Case Reports of Churg-Strauss Syndrome

    Directory of Open Access Journals (Sweden)

    Kamal Kishore Pandita

    2014-01-01

    Full Text Available Churg-Strauss syndrome (CSS is a rare cause of vasculitic neuropathy. Although rare and potentially fatal, Churg-Strauss syndrome (CSS is easily diagnosable and treatable. The presence of bronchial asthma with peripheral neuropathy in a patient alerts a physician to this diagnosis. This is vividly illustrated by the presented two cases who had neuropathy associated with bronchial asthma, eosinophilia, sinusitis, and positive perinuclear antineutrophil cytoplasmic antibodies (p-ANCA test, which improved with administration of steroids.

  20. The function and significance of SELENBP1 downregulation in human bronchial epithelial carcinogenic process.

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    Gu-Qing Zeng

    Full Text Available BACKGROUND: Our quantitative proteomic study showed that selenium-binding protein 1 (SELENBP1 was progressively decreased in human bronchial epithelial carcinogenic process. However, there is little information on expression and function of SELENBP1 during human lung squamous cell cancer (LSCC carcinogenesis. METHODS: iTRAQ-tagging combined with 2D LC-MS/MS analysis was used to identify differentially expressed proteins in the human bronchial epithelial carcinogenic process. SELENBP1, member of selenoproteins family and progressively downregulated in this process, was selected to further study. Both Western blotting and immunohistochemistry were performed to detect SELENBP1 expression in independent sets of tissues of bronchial epithelial carcinogenesis, and ability of SELENBP1 for discriminating NBE (normal bronchial epithelium from preneoplastic lesions from invasive LSCC was evaluated. The effects of SELENBP1 downregulation on the susceptibility of benzo(apyrene (B[a]P-induced human bronchial epithelial cell transformation were determined. RESULTS: 102 differentially expressed proteins were identified by quantitative proteomics, and SELENBP1 was found and confirmed being progressively decreased in the human bronchial epithelial carcinogenic process. The sensitivity and specificity of SELENBP1 were 80% and 79% in discriminating NBE from preneoplastic lesions, 79% and 82% in discriminating NBE from invasive LSCC, and 77% and 71% in discriminating preneoplastic lesions from invasive LSCC, respectively. Furthermore, knockdown of SELENBP1 in immortalized human bronchial epithelial cell line 16HBE cells significantly increased the efficiency of B[a]P-induced cell transformation. CONCLUSIONS: The present data shows for the first time that decreased SELENBP1 is an early event in LSCC, increases B[a]P-induced human bronchial epithelial cell transformation, and might serve as a novel potential biomarker for early detection of LSCC.

  1. DNA Extraction from Bronchial Aspirates for Molecular Cytology: Which Method to Take?

    OpenAIRE

    Grote, Hans Jürgen; Schmiemann, Viola; Sarbia, Mario; Böcking, Alfred

    2003-01-01

    Objective: To date, there are only few systematic reports on the quality of DNA extracted from routine diagnostic cytologic specimens. It was the aim of the present study to evaluate the ability of 50% ethanol/2% carbowax (Saccomanno fixative) to preserve bronchial secretions with high quality genomic DNA as well as to compare different DNA extraction methods. Methods: DNA was extracted from 45 bronchial aspirates by four different extraction protocols. Beside DNA yield, DNA quality with rega...

  2. Rhinovirus infection induces cytotoxicity and delays wound healing in bronchial epithelial cells

    OpenAIRE

    Constantopoulos Andreas G; Skevaki Chrysanthi L; Gourgiotis Dimitrios; Psarras Stelios; Bossios Apostolos; Saxoni-Papageorgiou Photini; Papadopoulos Nikolaos G

    2005-01-01

    Abstract Background Human rhinoviruses (RV), the most common triggers of acute asthma exacerbations, are considered not cytotoxic to the bronchial epithelium. Recent observations, however, have questioned this knowledge. The aim of this study was to evaluate the ability of RV to induce epithelial cytotoxicity and affect epithelial repair in-vitro. Methods Monolayers of BEAS-2B bronchial epithelial cells, seeded at different densities were exposed to RV serotypes 1b, 5, 7, 9, 14, 16. Cytotoxic...

  3. Incremental yield of bronchial washing for diagnosing smear-negative pulmonary tuberculosis

    OpenAIRE

    2013-01-01

    OBJECTIVE: To assess the increased diagnostic yield for pulmonary tuberculosis using bronchial washing cultures compared with sputum cultures. METHODS: Study conducted with 61 adults in Lima, Peru, from January 2006 to December 2007. The yield of sputum cultures was compared with the yield of acid-fast bacilli smears and cultures of bronchial washing for diagnosing pulmonary tuberculosis in suspected cases of clinical tuberculosis with negative acid fast bacilli sputum smears. RESULTS: ...

  4. Evaluation of Tracheal and Main Bronchial Diverticula Using Thin-Section MDCT

    Energy Technology Data Exchange (ETDEWEB)

    Jou, Sung Shick; Kim, Young Tong; Bae, Won Kyung; Kim, Il Yung; Kim, Hyung Hwan; Han, Jong Kyu [Soonchunhyang University Cheonan Hospital, Cheonan (Korea, Republic of)

    2010-02-15

    To evaluate the characteristics of tracheal and main bronchial diverticula in relation with emphysema. A total of 967 CT images were reconstructed with 1.25 mm axial images over 2 months. The incidence, size, number, and location of the tracheal and main bronchial diverticula were analyzed using 3D medical software (Seoul, Korea). The incidence of emphysema and the relationship between emphysema and the size of the diverticula were analyzed. In total, 50 patients (5.1%) showed tracheal diverticula in the right posterolateral wall. In addition, 51 patients (5.2%) showed 89 (9.4%) main bronchial diverticula in the inferior wall, while 68 (72%) showed diverticula in the left posterolateral wall. Tracheal diverticula (6.4 {+-} 5.0 mm, 1.0 {+-} 0.2) were larger and fewer than the main bronchial diverticula (2.1 {+-} 2.0 mm, 1.8 {+-} 1.6) (p<0.05). Moreover, tracheal diverticula (10.3 {+-} 7.4 mm) with emphysema in 13 patients (26%), were larger than those without emphysema (5.1 {+-} 3.0 mm) (p<0.05). On thin-section MDCT, the rates of incidence for tracheal and main bronchial diverticula are about 5%, respectively. Tracheal diverticula in the right posterolateral wall are smaller and fewer than the main bronchial diverticula, which are located primarily in the inferior wall of the left bronchus. Tracheal diverticula with emphysema are larger than those without emphysema.

  5. GATA-3 EXPRESSION IN PERIPHERAL BLOOD LYMPHOCYTES OF PATIENTS WITH BRONCHIAL ASTHMA

    Directory of Open Access Journals (Sweden)

    V. N. Mineev

    2010-01-01

    Full Text Available The aim of the study is to establish the features of expression of GATA-3 in peripheral lymphocytes from bronchial asthma patients (BA. Material and methods. 10 healthy controls, 15 patients with allergic (atopic and 15 persons with non-allergic BA were examined. A transcription factor GATA-3 expressed in peripheral lymphocytes was analyzed by Western blot after the lymphocytes were lysed. Preparation of cell lysates, and Western blotting were performed by means of a standard procedure (Amersham. An antibody against GATA-3 (Abcam, UK was used. Levels of the protein were analyzed versus β-actin levels using anti-actin antibody (Sigma Aldrich, USA. Results. Expression of GATA-3 was significantly increased in lymphocytes of patients with allergic BA as compared to healthy persons and non-allergic BA patients. The level of GATA-3 negatively correlated with the degree of airflow obstruction and positively correlated with dosage of parenteral steroids administered. Conclusion. GATA-3 may play a key role in the pathophysiology of BA. One may suggest that increased expression of GATA-3 transcription factor in atopic BA underlie high levels of Th2-cytokines production in allergic disease

  6. Airway Smooth Muscle as a Target in Asthma and the Beneficial Effects of Bronchial Thermoplasty

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    Luke J. Janssen

    2012-01-01

    Full Text Available Airflow within the airways is determined directly by the lumenal area of that airway. In this paper, we consider several factors which can reduce airway lumenal area, including thickening and/or active constriction of the airway smooth muscle (ASM. The latter cell type can also contribute in part to inflammation, another feature of asthma, through its ability to take on a synthetic/secretory phenotype. The ASM therefore becomes a strategically important target in the treatment of asthma, given these key contributions to the pathophysiology of that disease. Pharmacological approaches have been developed to elicit relaxation of the ASM, but these are not always effective in all patients, nor do they address the long-term structural changes which impinge on the airway lumen. The recent discovery that thermal energy can be used to ablate smooth muscle has led to the development of a novel physical intervention—bronchial thermoplasty—in the treatment of asthma. Here, we review the evolution of this novel approach, consider some of the possible mechanisms that account for its salutary effects, and pose new questions which may lead to even better therapies for asthma.

  7. Endovascular treatment of ectopic bronchial artery aneurysm with brachiocephalic artery stent placement and coil embolization

    Science.gov (United States)

    Di, Xiao; Ji, Dong-Hua; Chen, Yu; Liu, Chang-Wei; Liu, Bao; Yang, Juan

    2016-01-01

    Abstract Background: Bronchial artery aneurysm (BAA) is an uncommon but potentially life-threatening disease, and multiple BAAs are even rarer. Clinically, the tortuous and short neck of a BAA may present significant challenges for invasive intervention. Methods: This report describes the detailed process of diagnosis and treatment and includes a literature review of the etiology, clinical presentation, and therapeutic management of BAA. Results: A rare case of multiple BAAs, with one having an inflow artery arising from the brachiocephalic trunk, was referred to our hospital. The patient was successfully treated with coil embolization and brachiocephalic artery stent placement. In addition, we conducted a literature review involving 63 cases of BAA. BAA was most commonly associated with bronchiectasis and was located predominantly in the mediastinum. There was no significant difference between the diameters of the ruptured aneurysms and those of the nonruptured aneurysms (P = 0.115). Transcatheter arterial embolization was the most commonly adopted technique to treat BAA, while thoracic aortic endovascular repair was selected if the neck between the aneurysm and the aorta was short. Subgroup analysis suggested that patients with > 1 BAA were significantly more likely to be female than male (χ2 test, P = 0.034). Conclusion: Transcatheter coil embolization combined with stent placement could be a reasonable treatment option for BAAs with a tortuous and short neck. According to our literature review, patients with multiple BAAs display distinctive clinical characteristics compared with patients with a single BAA. PMID:27583854

  8. One-dimensional computational model of pulse wave propagation in the human bronchial tree.

    Science.gov (United States)

    Clavica, Francesco; Alastruey, Jordi; Borlotti, Alessandra; Sherwin, Spencer J; Khir, Ashraf W

    2010-01-01

    Airflow in the respiratory system has been predominantly studied in rigid ducts. Three-dimensional simulations are computationally expensive. One-dimensional (1-D) modelling offers a good compromise between accuracy and computational cost. In this work we described the propagation of air pulse in a model of human airways using the 1-D equations of flow in compliant vessels. Seven generations of bifurcations, starting from the trachea, were studied. Peripheral airways (from the 8(th) to 23(rd) generation) were modelled using lumped parameter models. Peripheral resistance values for normal and emphysematous lungs were taken from the literature. An acceleration pulse, very short in time, was enforced at the inlet of trachea. The results suggest that compression (positive pressure peaks) and expansion (negative pressure peaks) waves are generated according to the reflection coefficients of the corresponding reflection sites (bifurcations and terminal reflections). Different values for peripheral bronchial resistance generate three different terminal reflections, all negative with different wave amplitudes. The sensitivity of the code to different peripheral resistances suggests that the 1-D formulation is a promising tool for a better understanding of the impact of disease on the velocity and pressure waveforms in the first generations of airway vessels. PMID:21096163

  9. The Secretome of Human Bronchial Epithelial Cells Exposed to Fine Atmospheric Particles Induces Fibroblast Proliferation

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    Laurent Martinon

    2013-08-01

    Full Text Available Chronic exposure to particulate pollution is suspected to exacerbate inflammatory respiratory diseases such as asthma characterized by an airway remodelling involving fibrosis. Our study aims to investigate whether the secretome from human bronchial epithelial (HBE cells exposed to fine particulate matter (PM induces fibroblast proliferation. Primary HBE cells grown on air liquid interface were repeatedly exposed to fine PM at 5 and 10 µg/cm² (four treatments, 48 hours apart and maintained in culture for five weeks. Collected basolateral culture medium was used as a conditioned medium for the subsequent treatment of fibroblasts. We observed that the conditioned medium collected from HBE cells treated with fine PM increased the growth rate of fibroblasts compared to the conditioned medium collected from control HBE cells. Fibroblast phenotype assessed by the observation of the vimentin network was well preserved. The mitogenic effect of conditioned medium was reduced in the presence of anti-epidermal growth factor receptor (EGFR, anti-amphiregulin or anti-TGFa, underlining the role of EGFR ligands in fibroblast proliferation. When fibroblasts were co-cultured with HBE cells treated once with fine PM, they exhibited a higher growth rate than fibroblasts co-cultured with non-treated HBE cells. Altogether these data show that the exposure of HBE cells to fine PM induced the production of EGFR ligands in sufficient amount to stimulate fibroblast proliferation providing insight into the role of PM in airway remodelling.

  10. Anti-inflammatory effects of antibacterials on human bronchial epithelial cells

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    Hatz Rudolf

    2009-09-01

    Full Text Available Abstract Background Human Bronchial epithelial cells (hu-BEC have been claimed to play a significant role in the pathogenesis of chronic inflammatory airway diseases like COPD. In this context IL-8 and GM-CSF have been shown to be key cytokines. Some antibiotics which are routinely used to treat lower respiratory tract infections have been shown to exert additional immunomodulatory or anti-inflammatory effects. We investigated whether these effects can also be detected in hu-BEC. Methods Hu-BEC obtained from patients undergoing lung resections were transferred to air-liquid-interface (ALI culture. These cultures were incubated with cefuroxime (CXM, 10-62.5 mg/l, azithromycin (AZM, 0.1-1.5 mg/l, levofloxacin (LVX, 1-8 mg/l and moxifloxacin (MXF, 1-16 mg/l. The spontaneous and TNF-α (10 ng/ml induced expression and release of IL-8 and GM-CSF were measured using PCR and ELISA in the absence or presence of these antibiotics. Results The spontaneous IL-8 and GM-CSF release was significantly reduced with MXF (8 mg/l by 37 ± 20% and 45 ± 31%, respectively (both p Conclusion Using ALI cultures of hu-BEC we observed differential effects of antibiotics on spontaneous and TNF-α induced cytokine release. Our data suggest that MXF and AZM, beyond bactericidal effects, may attenuate the inflammatory process mediated by hu-BEC.

  11. Primary mechanism of the role of dual oxidase-1 causing airway allergic diseases in human bronchial epithelium%双重氧化酶1引起变应性疾病发生机制的初步研究

    Institute of Scientific and Technical Information of China (English)

    王丽芬; 黄志纯; 吴修法; 王海飞

    2013-01-01

    目的 探讨双重氧化酶-1 (dual oxidase-1,DUOX-1)在人气道上皮细胞(human bronchial epithelial cell)中导致气道高反应性的机制.方法 选择正常培养的人气道上皮细胞分为健康对照组、肿瘤坏死因子α(tumor necrosis factor-α,TNF-α)刺激组、甲基-β-环糊精(methyl-β-cyclodextrin,M-β-CD)+ TNF-α处理组、地昔帕明(desipramine,DES)+TNF-α处理组、二亚苯基碘(diphenylene iodonium,DPI)+TNF-α组、夹竹桃麻素(apocynin,APO)+TNF-α组.利用TNF-α作为刺激因素,采用蔗糖梯度离心的方法提取脂筏并应用免疫蛋白印记方法分析各组细胞膜上DUOX-1的含量;利用激光共聚焦显微镜观察细胞膜上DUOX-1的表达,同时观察其与脂筏标记蛋白霍乱毒素B亚单位(cholera toxin B subunit,CTXB)和神经酰胺(ceramide)的共定位现象;用活性氧试剂盒检测细胞内活性氧的生成.采用Sigmastat 3.02软件进行统计学处理.结果 ①活性氧的生成,对照组:l.00±0.00;TNF-α组:1.95±0.16;M-β-CD+TNF-α组:0.91±0.16; DES+ TNF-0组:1.49 ±0.20;DPI+TNF-α组:1.03±0.16;APO+ TNF-α组:1.47±0.26;六组差异有统计学意义(F=3.83,P<0.05).②DUOX-1蛋白的含量,对照组:0.21 ±0.02;TNF-α组:0.49±0.04;M-β-CD+TNF-α组:0.08±0.02;DES+TNF-α组:0.09±0.03;差异有统计学意义(F=3.96,P<0.05).③DUOX-1蛋白荧光值,对照组:1.72±0.21;TNF-α组:8.11±1.23;M-β-CD+ TNF-α组:1.51±0.32,DES+TNF-α组:1.43±0.11;差异有统计学意义(F =4.87,P<0.05).④DUOX-1蛋白基因检测,对照组:1.00±0.00;小胞浆RNA(small cytosol RNA,ScrRNA)+TNF-α组:1.75±0.04;DUOX-1ScrRNA+TNF-α组:1.15±0.02;差异有统计学意义(F=4.19,P<0.05).结论 TNF-α可以引起气道上皮细胞内DUOX-1在脂筏中的含量增加,并导致该酶的激活,从而致气道上皮细胞活性氧产生增加,引起气道高反应性,可能导致变应性疾病的发生;酸性鞘磷脂酶抑制剂DES可以抑制上述过程,说明这一过程

  12. Interleukin-17A and Toll-Like Receptor 3 Ligand Poly(I:C Synergistically Induced Neutrophil Chemoattractant Production by Bronchial Epithelial Cells.

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    Hirotaka Matsuzaki

    Full Text Available Chronic inflammatory airway diseases, such as bronchial asthma and chronic obstructive pulmonary disease, are common respiratory disorders worldwide. Exacerbations of these diseases are frequent and worsen patients' respiratory condition and overall health. However, the mechanisms of exacerbation have not been fully elucidated. Recently, it was reported that interleukin (IL-17A might play an important role in neutrophilic inflammation, which is characteristic of such exacerbations, through increased production of neutrophil chemoattractants. Therefore, we hypothesized that IL-17A was involved in the pathogenesis of acute exacerbation, due to viral infection in chronic inflammatory airway diseases. In this study, we assessed chemokine production by bronchial epithelial cells and investigated the underlying mechanisms. Comprehensive chemokine analysis showed that, compared with poly(I:C alone, co-stimulation of BEAS-2B cells with IL-17A and poly(I:C strongly induced production of such neutrophil chemoattractants as CXC chemokine ligand (CXCL8, growth-related oncogene (GRO, and CXCL1. Co-stimulation synergistically induced CXCL8 and CXCL1 mRNA and protein production by BEAS-2B cells and normal human bronchial epithelial cells. Poly(I:C induced chemokine expression by BEAS-2B cells mainly via Toll-like receptor 3/TIR-domain-containing adapter-inducing interferon-β-mediated signals. The co-stimulation with IL-17A and poly(I:C markedly activated the p38 and extracellular-signal-regulated kinase 1/2 pathway, compared with poly(I:C, although there was little change in nuclear factor-κB translocation into the nucleus or the transcriptional activities of nuclear factor-κB and activator protein 1. IL-17A promoted stabilization of CXCL8 mRNA in BEAS-2B cells treated with poly(I:C. In conclusion, IL-17A appears to be involved in the pathogenesis of chronic inflammatory airway disease exacerbation, due to viral infection by promoting release of neutrophil

  13. Prevention of Antigen-Induced Bronchial Hyperreactivity and Airway Inflammation in Sensitized Guinea-Pigs by Tacrolimus

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    J. R. Lapa e Silva

    1999-01-01

    Full Text Available We examined the effect of the immunosuppressive agent, tacrolimus (FK506, on antigen-induced bronchial hyperreactivity to acetylcholine and leukocyte infiltration into the airways of ovalbumin-challenged guinea-pigs. Subcutaneous injection of 0.5 mg/kg of FK506, 1 h before and 5 h after intra-nasal antigen challenge prevented bronchial hyperreactivity to aerosolized acetylcholine, eosinophilia in bronchoalveolar lavage (BAL fluid and bronchial tissue and the invasion of the bronchial wall by CD4+ T-lymphocytes. FK506 also suppressed ovalbumininduced increase in the number of leukocytes adhering to the pulmonary vascular endothelium and expressing α4-integrins. Inhibition by FK506 of antigen-induced bronchial hyperreactivity in sensitized guinea-pigs may thus relate to its ability to prevent the emergence of important inflammatory components of airway inflammation, such as eosinophil accumulation, as well as CD4+ T-lymphocyte infiltration into the bronchial tissue.

  14. [THE RELATIONSHIP BETWEEN DISORDERS-OF EXTERNAL RESPIRATION AND RIGHT HEART REMODELING IN PATIENTS WITH ATOPIC BRONCHIAL ASTHMA].

    Science.gov (United States)

    Solov'eva, I A; Sobko, E A; Ryazanova, N G; Krapohsina, A Yu; Gorgeeva, N V; Demko, I V

    2015-01-01

    This study aimed at the evaluation of the state of the respiratory system and its possible influence on the structural and functional characteristics of the right heart in patients with atopic bronchial asthma (BA) with a view to optimizing diagnostics and prevention of cardiovascular complications. The study included 189 subjects of whom 148 with BA were divided into 3 groups depending on the severity of the disease. Forty practically healthy volunteers comprised the control group. The external respiration function and right ventricle functional parameters were the main variables measured in all the participants of the study. It was shown that disorders of external respiration and pulmonary hyperinflation progressed with severity of BA and thereby promoted right ventricular myocardium remodeling and dysfunction that in turn led to chronic cardiac insufficiency. It is concluded that functional changes in the right heart in of patients with BA of different severity are associated with remodeling of the respiratory tract. PMID:26964462

  15. 支气管哮喘遗传学研究进展%Advancement of bronchial asthma in genetics studies

    Institute of Scientific and Technical Information of China (English)

    王春燕; 郭雪君

    2008-01-01

    过敏性支气管哮喘(简称哮喘)是儿童最常见的疾病之一,具有强烈的遗传异质性.哮喘及其表现型的很多遗传连锁性位点已被发现,这些位点可能是影响哮喘发病的主要原因.%Allergic bronchial asthma(asthma) is one of the most common diseases of childhood and is strongly genetic in origin. Many genome-wide screens for asthma and its associated traits have now been carried out,and genetic linkage has been consistently identified in several regions. It is probable that these loci contain major genes influencing atopy and asthma.

  16. Neutrophil migration and bronchial asthma%中性粒细胞迁移与支气管哮喘

    Institute of Scientific and Technical Information of China (English)

    李海银; 朱黎明

    2012-01-01

    Bronchial asthma (asthma) is a chronic airway inflammatory disease in which a variety of cells and cellular components participate.The etiology and pathogenesis of asthma is very complex.Recent studies found that neutrophils played an important role in the pathogenesis of asthma.Now the molecular mechanisms of neutrophil migration in asthma are summarized as follows.%支气管哮喘(简称哮喘)是由多种细胞和细胞组分参与的气道慢性炎症性疾病,其病因及发病机制十分复杂.近年来研究发现中性粒细胞在其发病机制中起重要作用,现就其迁移的分子机制在哮喘中的作用进行综述如下.

  17. [Dynamics of functional parameters in different schemes for bronchial asthma therapy: results of the STRELA-ACT multicentre study].

    Science.gov (United States)

    Ogorodova, L M; Kulikov, E S; Deev, I A; Cherniak, B A; Fassakhov, R S

    2011-01-01

    Different strategies for disease control in real clinical practice are compared in terms of dynamics of functional parameters in patients with persistent bronchial asthma. This prospective multicentre surveillance study was carried out in 19 Russian clinics using the common protocol. The patients were divided in 3 groups in accordance with the changes of basal antiinflammatory therapy during the study period. Group A--stepwise increase in the extent of combined salmoterol/fluticason therapy, group B--long-term stable-dose salmoterol/fluticason therapy, group C--salmoterol/fluticason therapy with gradual decrease of the dose and/or transition to an alternative variant. Statistical analysis using Statistica 6.0 program included data from 543 patients. The results suggest that the two first modalities increased the level of control (ACT test) and improved characteristics of external respiration throughout the study period. Strategy 3 was associated with a decrease in the external respiration function and the level of control.

  18. Undifferentiated Bronchial Fibroblasts Derived from Asthmatic Patients Display Higher Elastic Modulus than Their Non-Asthmatic Counterparts

    OpenAIRE

    Sarna, Michal; Wojcik, Katarzyna A.; Hermanowicz, Pawel; Wnuk, Dawid; Burda, Kvetoslava; Sanak, Marek; Czyż, Jarosław; Michalik, Marta

    2015-01-01

    During asthma development, differentiation of epithelial cells and fibroblasts towards the contractile phenotype is associated with bronchial wall remodeling and airway constriction. Pathological fibroblast-to-myofibroblast transition (FMT) can be triggered by local inflammation of bronchial walls. Recently, we have demonstrated that human bronchial fibroblasts (HBFs) derived from asthmatic patients display some inherent features which facilitate their FMT in vitro. In spite of intensive rese...

  19. Segmental bronchoprovocation in allergic rhinitis patients affects mast cell and basophil numbers in nasal and bronchial mucosa

    OpenAIRE

    Braunstahl, Gert-Jan; Overbeek, Shelley; Fokkens, Wytske; KleinJan, Alex; McEuen, A.R.; Walls, A F; Hoogsteden, Henk; Prins, Jan-Bas

    2001-01-01

    textabstractMast cells and basophils are cells that play an important role in the initiation and control of allergic inflammation in asthma and rhinitis. This study was undertaken to determine the presence and dynamics of mast cells and basophils in the nasal and bronchial mucosa of allergic rhinitis patients after segmental bronchial provocation (SBP). Eight nonasthmatic, grass pollen-allergic rhinitis patients and eight healthy controls were included. Bronchial and nasal biopsies, as well a...

  20. Analysis of the dynamic states of pulmonary ventilation and perfusion in bronchial asthmatics using [sup 133]Xe gas ventilation scintigraphy and [sup 99m]Tc-MAA lung perfusion scintigraphy

    Energy Technology Data Exchange (ETDEWEB)

    Ishihama, Hidenobu (Okayama Univ. (Japan). School of Medicine)

    1994-04-01

    To clarify the organic changes and pathophysiology of the lungs in intractable asthmatics, the dynamic states of pulmonary ventilation and perfusion were analyzed in 15 bronchial asthmatics in the stable state using [sup 133]Xe gas ventilation scintigraphy and [sup 99m]Tc-MAA lung perfusion scintigraphy, respectively. Dysfunction of pulmonary ventilation and impairment of pulmonary perfusion were significantly severer in intractable asthmatics than in non-intractable asthmatics (p<0.01, p<0.05, respectively) and those of asthma caused by aging. Late onset asthmatics with a long duration of disease tended to show severe impairment of pulmonary perfusion. Above all, the longer the duration of the disease, the severer became the impairment of pulmonary perfusion in late onset intractable asthmatics (LOIA). These findings suggest that the progression of irreversible organic changes of lungs in LOIA is involved in the severity of bronchial asthma. (author).

  1. [Efficacy of combined relaxation exercises for children with bronchial asthma].

    Science.gov (United States)

    Gröller, B

    1991-05-01

    In the framework of a pilot study, 15 children having bronchial asthma (4 female, 11 male; age 5-11;6) participated, over a period of 8 weeks, in two weekly sessions of combined relaxation, respiratory and sports exercises. The present article in particular focuses on the relaxation exercises, made up of Progressive Muscle Relaxation and Autogenic Training elements as well as of phantasy travels, mantras, and periodic music. Ongoing observation of the children during training, the findings of subsequent semi-structured interviews with them, topical instances of coping with impending asthma attacks by using the techniques learned, as well as the results of a catamnestic inquiry some three years later--all indicate a positive impact of the relaxation exercises. Statistical analysis of the data at hand revealed significant improvements in a number of pulmonary function parameters (airway resistance, forced expiratory volume for 1 s, forced vital capacity, peak expiratory flow rate). Interpretation of these findings must however take into account the entirety of the training provided.

  2. [Effectiveness of combined relaxation exercises for children with bronchial asthma].

    Science.gov (United States)

    Gröller, B

    1992-02-01

    In the framework of a pilot study, 15 children having bronchial asthma (4 female, 11 male; age 5-11; 6) participated, over a period of 8 weeks, in two weekly sessions of combined relaxation, respiratory and sports exercises. The present article in particular focuses on the relaxation exercises, made up of Progressive Muscle Relaxation and Autogenic Training elements as well as of phantasy travels, mantras, and periodic music. Ongoing observation of the children during training, the findings of subsequent semi-structured interviews with them, topical instances of coping with impending asthma attacks by using the techniques learnt, as well as the result of a catamnestic inquiry some three years later--all indicate a positive impact of the relaxation exercises. Statistical analysis of the data at hand revealed significant improvements in a number of pulmonary function parameters (airway resistance, forced expiratory volume for 1 s, forced vital capacity, peak expiratory flow rate). Interpretation of these findings must however take into account the entirety of the training provided.

  3. STAT6 EXPRESSION BY PERIPHERAL BLOOD LYMPHOCYTES IN BRONCHIAL ASTHMA

    Directory of Open Access Journals (Sweden)

    V. N. Mineev

    2007-01-01

    Full Text Available Abstract. The aim of present study was to determine the features of STAT6 and phospho-STAT6 (pSTAT6 expression in bronchial asthma (BA. Patients and methods. Eleven patients with allergic (atopic steroidfree were examined, five healthy controls served as a control. Expression of proteins (STAT6 and pSTAT6 in peripheral blood lymphocytes was studied by Western blot analysis after cell lysis. Preparation of cell lysates and Western blotting were performed using a standard procedure (Amersham. Antibodies against pSTAT6 and STAT6 (manufactured by Cell Signaling were used. Relative levels of specific proteins were analyzed using actin as a reference, by means of anti-actin antibody. Results. STAT6 phosphorylation was significantly increased in lymphocytes of patients with BA exacerbation, as compared to patients in remission and healthy group. The level of STAT6 was significantly higher compared to healthy persons and showed negative correlation with grade of air flow obstruction. Conclusion. STAT6 and their active form pSTAT6 may play a key role in BA pathophysiology. This study suggests atopic, steroid-free BA (in particular, on exacerbation to be associated with active cellular inflammatory process, involving activation of STAT6, along with increased level of their active form (pSTAT6. The work was supported by Saint-Petersburg government grants: PD04-4.0-102 (Certificate N ASP604079.

  4. Endoscopic bronchial valve treatment: patient selection and special considerations

    Directory of Open Access Journals (Sweden)

    Eberhardt R

    2015-10-01

    Full Text Available Ralf Eberhardt,1,2 Daniela Gompelmann,1,2 Felix JF Herth,1,2 Maren Schuhmann1 1Pneumology and Critical Care Medicine, Thoraxklinik at the University of Heidelberg, 2Translational Lung Research Center, Member of the German Center for Lung Research, Heidelberg, Germany Abstract: As well as lung volume reduction surgery, different minimally invasive endoscopic techniques are available to achieve lung volume reduction in patients with severe emphysema and significant hyperinflation. Lung function parameters and comorbidities of the patient, as well as the extent and distribution of the emphysema are factors to be considered when choosing the patient and the intervention. Endoscopic bronchial valve placement with complete occlusion of one lobe in patients with heterogeneous emphysema is the preferred technique because of its reversibility. The presence of high interlobar collateral ventilation will hinder successful treatment; therefore, endoscopic coil placement, polymeric lung volume reduction, or bronchoscopic thermal vapor ablation as well as lung volume reduction surgery can be used for treating patients with incomplete fissures. The effect of endoscopic lung volume reduction in patients with a homogeneous distribution of emphysema is still unclear and this subgroup should be treated only in clinical trials. Precise patient selection is necessary for interventions and to improve the outcome and reduce the risk and possible complications. Therefore, the patients should be discussed in a multidisciplinary approach prior to determining the most appropriate treatment for lung volume reduction. Keywords: lung emphysema, valve treatment, collateral ventilation, patient selection, outcome

  5. Endoscopic bronchial valve treatment: patient selection and special considerations.

    Science.gov (United States)

    Eberhardt, Ralf; Gompelmann, Daniela; Herth, Felix J F; Schuhmann, Maren

    2015-01-01

    As well as lung volume reduction surgery, different minimally invasive endoscopic techniques are available to achieve lung volume reduction in patients with severe emphysema and significant hyperinflation. Lung function parameters and comorbidities of the patient, as well as the extent and distribution of the emphysema are factors to be considered when choosing the patient and the intervention. Endoscopic bronchial valve placement with complete occlusion of one lobe in patients with heterogeneous emphysema is the preferred technique because of its reversibility. The presence of high interlobar collateral ventilation will hinder successful treatment; therefore, endoscopic coil placement, polymeric lung volume reduction, or bronchoscopic thermal vapor ablation as well as lung volume reduction surgery can be used for treating patients with incomplete fissures. The effect of endoscopic lung volume reduction in patients with a homogeneous distribution of emphysema is still unclear and this subgroup should be treated only in clinical trials. Precise patient selection is necessary for interventions and to improve the outcome and reduce the risk and possible complications. Therefore, the patients should be discussed in a multidisciplinary approach prior to determining the most appropriate treatment for lung volume reduction. PMID:26504379

  6. Effect of 4% lidocaine inhalation in bronchial asthma.

    Science.gov (United States)

    Prakash, G S; Sharma, S K; Pande, J N

    1990-01-01

    The effect of 4% lidocaine inhalation was studied in a single-blind fashion in 18 patients with chronic stable asthma. Inhalation of normal saline solution was used as placebo. None of the parameters except flow rate at 50% of vital capacity (V50) showed any statistically significant change from baseline values. V50 at 15 min was significantly lower (p less than 0.05) after 4% lidocaine inhalation. Considering more than 10% change from the baseline value as significant, 8 of 15 patients showed decrease in airway resistance (Raw) and 7 of the 15 patients showed an increase in specific airway conductance (SGaw) after 15 min of inhalation. However, V50 (8/18 patients), flow rate at 25% vital capacity [V25 (6/15 patients], and forced expiratory flow rate at 25-75% of the vital capacity (FEF25-75) (5/15 patients) showed a decrease after 15 min of 4% lidocaine inhalation. No change in pulmonary function was noted after 30 min of lidocaine inhalation. It is concluded from this study that lidocaine produces a small bronchodilatory effect on the large airways and a bronchoconstrictor effect on the small airways after 15 min of inhalation, but this effect is not statistically significant. It can be safely used as topical agent for bronchoscopy in patients with bronchial asthma.

  7. Prognostic factors in bronchial arterial embolization for hemoptysis

    Energy Technology Data Exchange (ETDEWEB)

    Kim, Eui Jong; Yoon, Yup; Oh, Joo Hyeong; Lim, Joo Won; Sung, Dong Wook [Kyung Hee University Hospital, Seoul (Korea, Republic of)

    1994-07-15

    To find the rebleeding factors in bronchial arterial embolization for treatment of hemoptysis, a retrospective study was performed. Medical records, angiographic findings and embolic materials of 35 patients who had undertaken arterial embolization for control of hemoptysis were reviewed. The period of follow-up for rebleeding was from 3 to 32 months after arterial embolization. We investigated the angiographic findings of extravasation, neovascularity, intervascular shunt, aneurysm and periarterial diffusion. Neovascularity was classified as mild(numerable neovascularity) and severe(innumerable). Rebleeding occurred in 15(43%) among 35 cases. Only two of 11 cases with no past episode of hemoptysis showed recurrence, while 9 of 15 cases who had more than three episodes did. Severe neovascularity were seen in 11 of 15 recurred cases, but seven of 20 non- recurred cases showed severe neovascularity. More than three angiographic findings representing hemoptysis were seen on 11(73%) among recurred 15 cases and seven(35%) among non- recurred 20 cases. The lesion was supplied by more than two different arteries on 8(54%) of the recurred cases, but only three(15%) of the non- recurred cases. Six of seven cases persistent neovascularity after arterial embolization were recurred. The history of repeated hemoptysis, severe neovascularity, variable angiographic findings, and post-embolization persistency of neovascularity were the factors related with the rebleeding after arterial embolization for hemoptysis. Careful and active arterial embolization are required on these conditions.

  8. Intestinal ischemia/reperfusion induces bronchial hyperreactivity and increases serum TNF-alpha in rats

    Directory of Open Access Journals (Sweden)

    Arruda Marcio Jose Cristiano de

    2006-01-01

    Full Text Available INTRODUCTION: Intestinal or hepatic ischemia/reperfusion induces acute lung injury in animal models of multiple organ failure. Tumor necrosis factor (TNF- alpha is involved in the underlying inflammatory mechanism of acute respiratory distress syndrome. Although the inflammatory cascade leading to acute respiratory distress syndrome has been extensively investigated, the mechanical components of acute respiratory distress syndrome are not fully understood. Our hypothesis is that splanchnic ischemia/reperfusion increases airway reactivity and serum TNF-alpha levels. OBJECTIVE: To assess bronchial smooth muscle reactivity under methacholine stimulation, and to measure serum TNF-alpha levels following intestinal and/or hepatic ischemia/reperfusion in rats. METHOD: Rats were subjected to 45 minutes of intestinal ischemia, or 20 minutes of hepatic ischemia, or to both (double ischemia, or sham procedures (control, followed by 120 minutes of reperfusion. The animals were then sacrificed, and the bronchial response to increasing methacholine molar concentrations (10-7 to 3 x 10-4 was evaluated in an ex-vivo bronchial muscle preparation. Serum TNF-alpha was determined by the L929-cell bioassay. RESULTS: Bronchial response (g/100 mg tissue showed increased reactivity to increasing methacholine concentrations in the intestinal ischemia and double ischemia groups, but not in the hepatic ischemia group. Similarly, serum TNF-alpha (pg/mL concentration was increased in the intestinal ischemia and double ischemia groups, but not in the hepatic ischemia group. CONCLUSION: Intestinal ischemia, either isolated or associated with hepatic ischemia, increased bronchial smooth muscle reactivity, suggesting a possible role for bronchial constriction in respiratory dysfunction following splanchnic ischemia/reperfusion. This increase occurred in concomitance with serum TNF-alpha increase, but whether the increase in TNF-alpha caused this bronchial contractility remains

  9. DNA methylation and bronchial asthma%DNA甲基化与支气管哮喘

    Institute of Scientific and Technical Information of China (English)

    徐礼; 向旭东

    2015-01-01

    Bronchial asthma (asthma) is a chronic inflammatory disease of the airways.In the pathogenesis of asthma,epigenetics links the environmental factors and genetic susceptibility,interrupting the differentiation and function of the immune system,thus contributing to the occurrence of asthma.DNA methylation is known as the most common form of epigenetics.Food and environmental factors affect the differentiation of CD4+ T cells among the cellular components of asthma by DNA methylation.Maternal prenatal consumption of diet rich in methyl donors,and inhalation of risk factors in the environment can affect DNA methylation in CD4+ T cells in vivo,resulting in "Th2 polarization" and promoting the disease of asthma.%支气管哮喘(简称哮喘)是一种气道慢性炎症性疾病,在哮喘的发病机制中,表观遗传学是联系环境因素与遗传易感因素之间的纽带,导致机体免疫系统的发育及功能紊乱,从而促进哮喘的发生.DNA甲基化作为最常见的表观遗传修饰形式,食物与环境因素通过DNA甲基化产生对哮喘细胞学组分CD4+T细胞分化的影响.母体产前食用富含甲基供体饮食,吸入环境中的危险因子均可影响CD4+T细胞在体内的DNA甲基化,导致“Th2极化”,促进哮喘的发生.

  10. The low PLC-δ1 expression in cystic fibrosis bronchial epithelial cells induces upregulation of TRPV6 channel activity.

    Science.gov (United States)

    Vachel, Laura; Norez, Caroline; Jayle, Christophe; Becq, Frédéric; Vandebrouck, Clarisse

    2015-01-01

    Increase of Ca(2+) influx in Cystic Fibrosis (CF) cells has been reported to be related to Transient Receptor Potential Canonical (TRPC6) channel, which is implicated in a functional coupling with Cystic Fibrosis Transmembrane conductance Regulator (CFTR). Several members of the Transient Receptor Potential Vanilloid (TRPV) channels family have already been described as emerging target for respiratory diseases. Two specific isoforms, TRPV5 and TRPV6 are of particular interest in the context of CF Ca(2+) homeostasis as they are highly selective toward Ca(2+) and constitutively activated. Thus, we investigated the involvement of these channels in Ca(2+) influx in CF and non-CF human bronchial epithelial cell lines. 16HBE14o-, CFBE41o- cell lines, primary human airway epithelial cells (hAEC) and freshly isolated human airway epithelial cells from CF and non-CF individuals were used. We showed that both channels are expressed in CF and non-CF cells and constitutive Ca(2+) influx was significantly higher (85%) in cells from CF individuals compared to cells from non-CF ones. Using the selective inhibitor of TRPV6 channel SOR-C27 and a siRNA strategy, our results revealed that TRPV6 was mostly involved in the increase of Ca(2+) influx. TRPV6 channel is negatively regulated by the PLC-PIP2 pathway. We measured the Ca(2+) influx in the presence of the non-specific PLC inhibitor, U73122, in non-CF human bronchial epithelial cells. Ca(2+) influx was increased by 33% with U73122 and this increase was largely reduced in the presence of SOR-C27. PLC inhibition in CF cells by U73122 had no effect on Ca(2+) influx. These results showed that PLC-PIP2 pathway is dysregulated in CF cells and leads to the increase of TRPV6 activity. The regulation of TRPV6 by PLC-PIP2 pathway implicates the specific PLC isoform, PLC-δ1. Immunoblot experiments revealed that expression of PLC-δ1 was decreased by 70% in CF cells. TRPV6 activity was normalized but not the level of expression of PLC-δ1

  11. The measurement of tracheo-bronchial mucociliary clearance with 99mTc-DTPA aerosol scintigraphy and its preliminary application in COPD patients

    International Nuclear Information System (INIS)

    Mucocoiliary clearance of respiratory channels is one of the important mechanisms guarding against retention of foreign particles within the lungs. Disorders of the mucociliary transport system play a major role among non-respiratory function in causing congenital and acquired bronchial disease. Thus, objective assay of the system is essential to recognizing and understanding abnormalities. In the present paper, a simple, noninvasive, and reliable in vivo method of monitoring mucociliary clearance function is reported. 18 healthy subjects and 32 COPD patients were studied with 99mTc-DTPA aerosol scintigraphy. Monitoring was performed by visual inspection (cinescintigraphy, to observe the distribution of 99mTc-DTPA particles and the movement of radiomucous 'hot bolus') and quantitative analysis (two indexes were utilized: the first is airway clearance ratio (ACR); the second is mucociliary clearance rate (MCC), i.e. speed of advances of mucous bolus, calculated reported by Zwas). Siemens 3700 SPECT, 64 X 64 byte mode, zoom 2. Sequential images (frame/60s) were obtained for 120 minutes. The deposition pattern of 99mTc-DTPA particles in normal subjects was uniform. The deposition pattern in COPD patients demonstrated in general a centrally located distribution with major retention in the proximal airways. Four abnormal mucous transport patterns were regionally observed: stasis, regurgitation, straying and spiral or zigzag transport. Statistical analysis showed there was a significant difference of ACR between healthy subjects and COPD patients at different time points (P<0.05). The MCC in healthy subjects and COPD patients was 3.89±0.92 mm/min and 1.32±0.59 mm/min respectively. The method of assaying tracheo-bronchial mucociliary clearance reported here is simple and objective. It has not only the advantage of visual inspection and quantitative analysis, but also has a potential usefulness in studying other bronchial diseases and evaluating of therapeutic

  12. Application of adjusted subpixel method (ASM) in HRCT measurements of the bronchi in bronchial asthma patients and healthy individuals

    International Nuclear Information System (INIS)

    Background: Recently, we described a model system which included corrections of high-resolution computed tomography (HRCT) bronchial measurements based on the adjusted subpixel method (ASM). Objective: To verify the clinical application of ASM by comparing bronchial measurements obtained by means of the traditional eye-driven method, subpixel method alone and ASM in a group comprised of bronchial asthma patients and healthy individuals. Methods: The study included 30 bronchial asthma patients and the control group comprised of 20 volunteers with no symptoms of asthma. The lowest internal and external diameters of the bronchial cross-sections (ID and ED) and their derivative parameters were determined in HRCT scans using: (1) traditional eye-driven method, (2) subpixel technique, and (3) ASM. Results: In the case of the eye-driven method, lower ID values along with lower bronchial lumen area and its percentage ratio to total bronchial area were basic parameters that differed between asthma patients and healthy controls. In the case of the subpixel method and ASM, both groups were not significantly different in terms of ID. Significant differences were observed in values of ED and total bronchial area with both parameters being significantly higher in asthma patients. Compared to ASM, the eye-driven method overstated the values of ID and ED by about 30% and 10% respectively, while understating bronchial wall thickness by about 18%. Conclusions: Results obtained in this study suggest that the traditional eye-driven method of HRCT-based measurement of bronchial tree components probably overstates the degree of bronchial patency in asthma patients.

  13. Captopril augments acetylcholine-induced bronchial smooth muscle contractions in vitro via kinin-dependent mechanisms.

    Science.gov (United States)

    Agrawal, Naman; Akella, Aparna; Deshpande, Shripad B

    2016-06-01

    Angiotensin converting enzyme (ACE) inhibitors therapy is aassociated with bothersome dry cough as an adverse effect. The mechanisms underlying this adverse effect are not clear. Therefore, influence of captopril (an ACE inhibitor) on acetylcholine (ACh)-induced bronchial smooth muscle contractions was investigated. Further, the mechanisms underlying the captopril-induced changes were also explored. In vitro contractions of rat bronchial smooth muscle to cumulative concentrations of ACh were recorded before and after exposure to captopril. Further, the involvement of kinin and inositol triphosphate (IP₃) pathways for captopril-induced alterations were explored. ACh produced concentration-dependent (5-500 µM) increase in bronchial smooth muscle contractions. Pre-treatment with captopril augmented the ACh-induced contractions at each concentration significantly. Pre-treatment with aprotinin (kinin synthesis inhibitor) or heparin (inositol triphosphate, IP₃-inhibitor), blocked the captopril-induced augmentation of bronchial smooth muscle contractions evoked by ACh. Further, captopril-induced augmentation was absent in calcium-free medium. These results suggest that captopril sensitizes bronchial smooth muscles to ACh-induced contractions. This sensitization may be responsible for dry cough associated with captopril therapy. PMID:27468462

  14. Heart, tracheo-bronchial and thoracic spine trauma. Succesful multidisciplinary management: a challenging thoracic politrauma

    Directory of Open Access Journals (Sweden)

    Sergio Nicola Forti Parri

    2014-01-01

    Full Text Available We reported the case of a 36 years old woman involved in a car accident and admitted to the Emergency Room with critical conditions. A CT scan showed hemopericardium, pneumomediastinum and D2 unstable vertebral fracture; then a sternotomy was promptly performed. After admittance to Intensive Care Unit a bronchoscopy showed a tear of the posterior wall of the trachea and the complete disruption of the left main bronchus with a 2 cm gap beetwen two consecutive cartilage rings. D2 fracture would have required stabilization, but pronation of the patient was contraindicated by the bronchial rupture. On the nineth day the vertebral fracture was stabilized, thus allowing a lateral decubitus and a left thoracotomy. The bronchial laceration was wrapped all around with a pedicled pericardial flap and a bronchial stent was placed inside the gap with a pediatric bronchoscope. Postoperative course was uneventful and the patient was transferred to the Physical Rehabilitation Unit after 23 d. The successful outcome of this case is the result of multidisciplinary management where every decision was shared by each specialist. From the surgical point of view survival is uncommon in such severe association of lesions. The use of pericardium wrap together with a bronchial stent represents an innovative solution to treat a complicated bronchial disruption.

  15. Heart, tracheo-bronchial and thoracic spine trauma. Succesful multidisciplinary management:a challenging thoracic politrauma

    Institute of Scientific and Technical Information of China (English)

    Sergio Nicola Forti Parri; Gian Marco Guiducci; Kenji Kawamukai; Carlo Coniglio; Giovanni Gordini; Federico De Iure; Rocco Trisolini; Salomone Di Saverio; Gregorio Tugnoli

    2014-01-01

    We reported the case of a36 years old woman involved in a car accident and admitted to theEmergencyRoom with critical conditions.ACT scan showed hemopericardium, pneumomediastinum andD2 unstable vertebral fracture; then a sternotomy was promptly performed.After admittance toIntensiveCareUnit a bronchoscopy showed a tear of the posterior wall of the trachea and the complete disruption ofthe left main bronchus with a2 cm gap beetwen two consecutive cartilage rings.D2 fracture would have required stabilization, but pronation of the patient was contraindicated by the bronchial rupture.On the nineth day the vertebral fracture was stabilized, thus allowing a lateral decubitus and a left thoracotomy.The bronchial laceration was wrapped all around with a pedicled pericardial flap and a bronchial stent was placed inside the gap with a pediatric bronchoscope.Postoperative course was uneventful and the patient was transferred to thePhysicalRehabilitationUnit after23 d.The successful outcome of this case is the result of multidisciplinary management where every decision was shared by each specialist. From the surgical point of view survival is uncommon in such severe association of lesions.The use of pericardium wrap together with a bronchial stent represents an innovative solution to treat a complicated bronchial disruption.

  16. Bronchial artery embolization for therapy of pulmonary bleeding in patients with cystic fibrosis

    International Nuclear Information System (INIS)

    Introduction: Acute pulmonary emergencies in patient with cystic fibrosis (CF) can be found in cases of pneumothorax as well as hemoptysis. If the bleeding cannot be stopped by conservative methods, an embolization of the bronchial arteries should be done. Materials and Method: 11 patients were embolized using a combination of PVA particles and microcoils. Results: From January 1996 to June 2001 17 bronchial arteries in 11 patients were embolized. 7 patients suffered from chronical hemoptysis, 4 patients had an acute hemoptysis. In 4 patients both sides were embolized, in 3 patients only one side. The remaining 4 patients needed a second intervention, embolizing the other side. The primary embolizated bronchial artery was still closed in all 4 patients. In 1 patient the selective catheterization of a bronchial artery was not successful, thus the embolization could not be carried out. 1 patient died 5 days after the intervention due to a fulminant pneumonia (Pseudomonas aeruginosa) without recurrent bleeding. In two patients atypical branches from intercostal arteries feeding the bronchial arteries were detected and successfully embolized. All patients profited from the therapy, as bleeding could be stopped or at least be reduced. 3 patients suffered from back pain during or after intervention. There were no severe complications like neurological deficiencies or necroses. (orig.)

  17. Distribution of radioactive aerosol in the airways of children and adolescents with bronchial hyper-responsiveness

    International Nuclear Information System (INIS)

    The purpose of this study was to examine the relationship between the pulmonary distribution of inhaled radioaerosol, bronchial responsiveness, and lung function in children and adolescents. The participating subjects were divided into three groups: (1) 14 asthmatics with bronchial hyper-responsiveness (BHR), (2) five non-asthmatic subjects with BHR, and (3) 20 controls without BHR. Pulmonary distribution of [99Tcm] albumin radioaerosol, maximal expiratory flow when 25% of forced vital capacity remain to be exhaled (MEF25), and bronchial responsiveness to inhaled histamine were measured. Twenty subjects (52%) has irregular central distribution and 19 subjects (48%) had regular distribution of radioaerosol in their lungs. No difference in distribution of radioaerosol was found between the three groups of children. The median MEF25 among non-asthmatic subjects (80% predicted) was lower than that found in controls (92% predicted) but higher than that found in asthmatic subjects (55% predicted). A relationship was found between reduced flow at the peripheral airways, as indicated by MEF25 and the degree of central distribution of radioaerosol. Furthermore, subjects with irregular central distribution of radioaerosol had an increase degree of bronchial responsiveness. In conclusion, children and adolescents who have flow rates in the peripheral airways or increased degree of bronchial responsiveness tend to have abnormal distribution of radioaerosols. (author)

  18. Cardiopulmonary disease in the geriatric dog and cat

    International Nuclear Information System (INIS)

    The incidence of cardiopulmonary disease increases with age. Degenerative valvular disease, chronic obstructive pulmonary disease, and arrhythmias are common in the geriatric dog. Chronic bronchial disease, pulmonary neoplasia, and arrhythmias occur in the geriatric cat. Systemic diseases in both species often show cardiopulmonary manifestations. Medical management to treat the underlying disease and to control clinical signs is complicated by altered absorption, metabolism, and elimination of drugs

  19. Endoscopic anatomy and map of the equine bronchial tree.

    Science.gov (United States)

    Smith, B L; Aguilera-Tejero, E; Tyler, W S; Jones, J H; Hornof, W J; Pascoe, J R

    1994-07-01

    To develop a bronchoscopic map of the equine respiratory tree, the major airways of the lungs of 6 healthy Thoroughbred horses were systematically explored with a flexible fibreoptic endoscope through a tracheostomy while the horses were sedated in stocks. With the carina as the reference point, measurements were made of distances to the branches of the major airways using markers on the shaft of the endoscope. All branches were explored until the narrowing of their diameters prevented further advancement of the endoscope. Positions of origins of branches from the parent bronchus were recorded in relation to a 12 h clock. Branching patterns of the right and left lungs were similar. Seventeen branches of the principal and caudal lobar bronchi of the left lung, and 18 branches of the principal and caudal lobar bronchi of the right lung were identified. Mean explorable distances from the carina to the ends of the right and left caudal lobar bronchi were 34.0 +/- 3.5 (sd) and 34.5 +/- 3.0 cm, respectively. Generally, smaller horses had shorter explorable bronchial lengths. Branching patterns of the parent bronchi were fairly consistent among horses, particularly the branches closest to the carina. After endoscopy and euthanasia, the lungs were removed, and dried with pressurised air flowing through them for 7-10 days. Attempts to explore the airways of the dried lungs endoscopically were relatively unsuccessful, because airways were much smaller in the dried lungs, and many of the branches were distorted when compared with their antemortem appearances. However, having a dried lung specimen as a reference during the bronchoscopic procedure was useful for maintaining orientation in the lungs. Radiographs were used to estimate the location of the origin and destination of each airway branch in relation to the nearest intercostal space. This makes the airway map useful when lesions identified radiographically are to be lavaged. PMID:8575395

  20. Differential deposition of fibronectin by asthmatic bronchial epithelial cells.

    Science.gov (United States)

    Ge, Qi; Zeng, Qingxiang; Tjin, Gavin; Lau, Edmund; Black, Judith L; Oliver, Brian G G; Burgess, Janette K

    2015-11-15

    Altered ECM protein deposition is a feature in asthmatic airways. Fibronectin (Fn), an ECM protein produced by human bronchial epithelial cells (HBECs), is increased in asthmatic airways. This study investigated the regulation of Fn production in asthmatic or nonasthmatic HBECs and whether Fn modulated HBEC proliferation and inflammatory mediator secretion. The signaling pathways underlying transforming growth factor (TGF)-β1-regulated Fn production were examined using specific inhibitors for ERK, JNK, p38 MAPK, phosphatidylinositol 3 kinase, and activin-like kinase 5 (ALK5). Asthmatic HBECs deposited higher levels of Fn in the ECM than nonasthmatic cells under basal conditions, whereas cells from the two groups had similar levels of Fn mRNA and soluble Fn. TGF-β1 increased mRNA levels and ECM and soluble forms of Fn but decreased cell proliferation in both cells. The rate of increase in Fn mRNA was higher in nonasthmatic cells. However, the excessive amounts of ECM Fn deposited by asthmatic cells after TGF-β1 stimulation persisted compared with nonasthmatic cells. Inhibition of ALK5 completely prevented TGF-β1-induced Fn deposition. Importantly, ECM Fn increased HBEC proliferation and IL-6 release, decreased PGE2 secretion, but had no effect on VEGF release. Soluble Fn had no effect on cell proliferation and inflammatory mediator release. Asthmatic HBECs are intrinsically primed to produce more ECM Fn, which when deposited into the ECM, is capable of driving remodeling and inflammation. The increased airway Fn may be one of the key driving factors in the persistence of asthma and represents a novel, therapeutic target. PMID:26342086

  1. Virodhamine and CP55,940 modulate cAMP production and IL-8 release in human bronchial epithelial cells

    NARCIS (Netherlands)

    Gkoumassi, E.; Dekkers, B. G. J.; Droege, M. J.; Elzinga, C. R. S.; Schmidt, M.; Meurs, H.; Zaagsma, J.; Nelemans, S. A.

    2007-01-01

    Background and purpose: We investigated expression of cannabinoid receptors and the effects of the endogenous cannabinoid virodhamine and the synthetic agonist CP55,940 on cAMP accumulation and interleukin-8 (IL-8) release in human bronchial epithelial cells. Experimental approach: Human bronchial e

  2. Bronchial responsiveness to adenosine 5 '-monophosphate (AMP) and methacholine differ in their relationship with airway allergy and baseline FEV

    NARCIS (Netherlands)

    De Meer, G; Heederik, D; Postma, DS

    2002-01-01

    Bronchial hyperresponsiveness (BHR) and inflammation are central hallmarks of asthma. Studies in patients with asthma suggest that BHR to adenosine 5'-monophosphate (AMP) is a better marker of bronchial inflammation than BHR to methacholine. The association between markers of airway inflammation and

  3. [A case of pulmonary sarcoidosis complicated with bronchial asthma that proved eosinophilic invasion].

    Science.gov (United States)

    Harada, Tomoya; Kawasaki, Yuji; Touge, Hirokazu; Tokuyasu, Hirokazu; Yamasaki, Akira; Shimizu, Eiji

    2012-12-01

    A 33-year-old man complaining of cough admitted our hospital for examination of bilateral hilar and mediastinal lymphadenopathy. He diagnosed pulmonary sarcoidosis, because of elevation of serum angiotensin converting enzyme (ACE), epitheloid granuloma with noncaseating necrosis from transbronchial lung biopsy (TBLB) specimen, increasing of lymphocyte and elevation of the CD4/CD8 ratio in bronchoalveolar lavage fluid (BALF). Furthermore, eosinophil ratio in BALF was 3%, hyperplasia of goblet cell, eosinophilic invasion to bronchial epithelium, and thickened basal membrane were found in same biopsy specimen. He had mild reversible airway obstruction. He was diagnosed pulmonary sarcoidosis complicated with bronchial asthma. Sarcoidosis is characteristic of the T helper type 1 (Th1) mediated immune response, and bronchial asthma is characteristic of the Th2. This case histopathologically revealed that both Th1 mediated immune response and Th2 could be coexisted. PMID:23466616

  4. Pulmonary nodules: optimal slice thickness of CT in revealing bronchial imageology

    Institute of Scientific and Technical Information of China (English)

    Shenjiang Li; Yuanyuan Wang; Changcheng Li; Xing Wang; Debin Liu; Wenjie Liang; Feng Zhu; Yan Zhu; Xuefeng Cui; Fangang Hu

    2011-01-01

    Objective: The aim of our study was to determine an optimal slice thickness that was efficient in revealing bronchial imageology of pulmonary nodules (PNs) on multi-slice spiral computed tomography (MSCT) images preliminarily. Methods: Fifty-four patients with 62 PNs (diameter ≤ 3 cm) underwent multidetector-row computed tomography of the chest in a single-breath-hold technique. The raw data were acquired with a collimation of 0.625 mm. Three sets of contiguous im-ages were reconstructed with 1-, 2-, and 5-mm slice thickness, respectively. Bronchial imageology of SPNs on the CT images presented in 1-, 2-, and 5-mm slice thickness was compared. Using the 1-mm sections as the gold standard, an optimal slice thickness in revealing bronchial imageology of PNs was determined. Results: Bronchial imageology of PNs on the CT im-ages presented in 1 mm slice thickness involved 85 bronchi (35 second-fourth generation bronchi; 50 fifth-eighth generation bronchi). Bronchial imageology on 2-mm-thick sections was as same as that on 1-mm-thick sections in 34 second- fourth generation bronchi. No statistically significant difference in number of second- fourth generation bronchi with same bronchial imageology was found between that on 2-mm-thick images and 1-mm-thick images (P = 0.836 > 0.05). Bronchial imageology on 5-mm-thick sections was as same as that on 1-mm-thick sections in 24 second-fourth generation bronchi. There was statistically significant difference in number of second-fourth generation bronchi with same bronchial imageology between that on 5-mm-thick images and 1-mm-thick images (P = 0.026 < 0.05). Bronchial imageology on 2-mm-thick sections was as same as that on 1-mm-thick sections in 38 fifth-eighth generation bronchi. There was statistically significant difference in number of fifth-eighth generation bronchi with same bronchial imageology between that on 2-mm-thick images and 1-mm-thick images (P = 0.029 < 0.05). Bronchial imageology on 5-mm-thick images was

  5. Secondhand smoke exposure causes bronchial hyperreactivity via transcriptionally upregulated endothelin and 5-hydroxytryptamine 2A receptors

    DEFF Research Database (Denmark)

    Cao, Lei; Zhang, Yaping; Cao, Yong-Xiao;

    2012-01-01

    with a sensitive myograph system. The mRNA transcription and protein translation of the target receptors and the kinases in Raf/ERK/MAPK pathway were investigated by real-time PCR, Western blotting and immunofluorescence, respectively. Compared with exposure to fresh air, SHS induced enhanced bronchial contractile......BACKGROUND: Cigarette smoke exposure is strongly associated with airway hyperreactivity (AHR) which is the main characteristic seen in asthma. The intracellular MAPK signaling pathways are suggested to be associated with the airway damage to the AHR. In the present study, we hypothesize...... that secondhand cigarette smoke (SHS) exposure upregulates the bronchial contractile receptors via activation of the Raf/ERK/MAPK pathway. METHODOLOGY/PRINCIPAL FINDINGS: Rats were exposed to SHS for 3 h daily for up to 8 weeks. The receptor agonists-induced bronchial contractile reactivity was analyzed...

  6. Eosinophilic Pneumonia in a Patient with Bronchial Myiasis; Case report and literature review

    Directory of Open Access Journals (Sweden)

    Arindom Aich

    2015-11-01

    Full Text Available Pulmonary myiasis is an unusual form of myiasis in humans and has been recently identified as a cause of eosinophilic pneumonia. We report the case of a 13-year-old Omani boy who presented to the Royal Hospital, Muscat, Oman, in October 2014 with respiratory distress. Bronchial aspirates revealed features of eosinophilic pneumonia. Possible larvae identified in the cytology report, a high immunoglobulin E level and the patient history all indicated bronchial myiasis. The patient was treated with steroids and ventilation and has since been diseasefree with no long-term side-effects. To the best of the authors’ knowledge, this is the first case of bronchial myiasis in Oman.

  7. [Suggesting the Significance of Pericardial Fat Pad in Bronchial Stump Fistula].

    Science.gov (United States)

    Fukuoka, Tomoki; Sano, Masaaki; Tominaga, Nasa; Sanada, Shotaro; Uno, Yasuo; Oya, Hisaharu; Nishi, Tetsuo; Koshikawa, Katsumi

    2016-05-01

    Bronchial stump fistula is a post-operative complication with poor outcome after pulmonary lobectomy. In order to prevent this complication, the bronchial stump is covered with pericardial fat tissue in our hospital. The case was 58 year old male who received adjuvant chemotherapy after sigmoidectomy for sigmoid colon cancer. As he developed multiple pulmonary metastases, 48 courses of chemotherapy were performed. The lesions had been localized at the right lower lobe, and neither increase in the size of these lesions nor development of other lesions were observed. Hence, an operation was performed. After right lower lobectomy, the bronchial stump was covered with the pericardial fat tissue. Three months after the operation, he developed pneumothorax, and bubbles were detected inside the fat. The pneumothorax was cured conservatively, and the bubbles disappeared spontaneously after 10 months. It is rare that the patient with bubbles in the covering tissue observed for a long time is cured conservatively, suggesting the significance of the stump pad.

  8. Pulmonary artery stent for bronchial adenoid cystic carcinoma causing pulmonary artery stenosis

    DEFF Research Database (Denmark)

    Smith, Corey Allister; Kotlyar, Eugene; Mellemkjaer, Soren;

    2014-01-01

    A 46-year-old woman presented with a 6-month history of dyspnea and weight loss on a background of previous pneumonectomy for bronchial adenoid cystic carcinoma 14 years beforehand. Several years prior to this presentation, she had developed left vocal cord palsy and a metastatic lesion to the ri......A 46-year-old woman presented with a 6-month history of dyspnea and weight loss on a background of previous pneumonectomy for bronchial adenoid cystic carcinoma 14 years beforehand. Several years prior to this presentation, she had developed left vocal cord palsy and a metastatic lesion...... improved both pulmonary artery pressures and the patient's symptoms. The diagnosis of pulmonary artery stenosis due to mediastinal infiltration by metastatic bronchial adenoid cystic carcinoma was based on these findings as well as the presence of the pulmonary nodules and the previous mediastinal...

  9. Influenza H5N1 and H1N1 virus replication and innate immune responses in bronchial epithelial cells are influenced by the state of differentiation.

    Directory of Open Access Journals (Sweden)

    Renee W Y Chan

    Full Text Available Influenza H5N1 virus continues to be enzootic in poultry and transmits zoonotically to humans. Although a swine-origin H1N1 virus has emerged to become pandemic, its virulence for humans remains modest in comparison to that seen in zoonotic H5N1 disease. As human respiratory epithelium is the primary target cells for influenza viruses, elucidating the viral tropism and host innate immune responses of influenza H5N1 virus in human bronchial epithelium may help to understand the pathogenesis. Here we established primary culture of undifferentiated and well differentiated normal human bronchial epithelial (NHBE cells and infected with highly pathogenic influenza H5N1 virus (A/Vietnam/3046/2004 and a seasonal influenza H1N1 virus (A/Hong Kong/54/1998, the viral replication kinetics and cytokine and chemokine responses were compared by qPCR and ELISA. We found that the in vitro culture of the well differentiated NHBE cells acquired the physiological properties of normal human bronchi tissue which express high level of alpha2-6-linked sialic acid receptors and human airway trypsin-like (HAT protease, in contrast to the low expression in the non-differentiated NHBE cells. When compared to H1N1 virus, the H5N1 virus replicated more efficiently and induced a stronger type I interferon response in the undifferentiated NHBE cells. In contrast, in well differentiated cultures, H5N1 virus replication was less efficient and elicited a lower interferon-beta response in comparison with H1N1 virus. Our data suggest that the differentiation of bronchial epithelial cells has a major influence in cells' permissiveness to human H1N1 and avian H5N1 viruses and the host innate immune responses. The reduced virus replication efficiency partially accounts for the lower interferon-beta responses in influenza H5N1 virus infected well differentiated NHBE cells. Since influenza infection in the bronchial epithelium will lead to tissue damage and associate with the

  10. Mechanism of cigarette smoke condensate-induced acute inflammatory response in human bronchial epithelial cells

    Directory of Open Access Journals (Sweden)

    Mohapatra Shyam S

    2002-07-01

    Full Text Available Abstract Background To demonstrate the involvement of tobacco smoking in the pathophysiology of lung disease, the responses of pulmonary epithelial cells to cigarette smoke condensate (CSC — the particulate fraction of tobacco smoke — were examined. Methods The human alveolar epithelial cell line A549 and normal human bronchial epithelial cells (NHBEs were exposed to 0.4 μg/ml CSC, a concentration that resulted in >90% cell survival and Results NHBEs exposed to CSC showed increased expression of the inflammatory mediators sICAM-1, IL-1β, IL-8 and GM-CSF, as determined by RT-PCR. CSC-induced IL-1β expression was reduced by PD98059, a blocker of mitogen-actived protein kinase (MAPK kinase (MEK, and by PDTC, a NFκB inhibitor. Analysis of intracellular signaling pathways, using antibodies specific for phosphorylated MAPKs (extracellular signal-regulated kinase [ERK]-1/2, demonstrated an increased level of phosphorylated ERK1/2 with increasing CSC concentration. Nuclear localization of phosphorylated ERK1/2 was seen within 30 min of CSC exposure and was inhibited by PD98059. Increased phosphorylation and nuclear translocation of IκB was also seen after CSC exposure. A549 cells transfected with a luciferase reporter plasmid containing a NFκB-inducible promoter sequence and exposed to CSC (0.4 μg/ml or TNF-α (50 ng/ml had an increased reporter activity of approximately 2-fold for CSC and 3.5-fold for TNF-α relative to untreated controls. Conclusion The acute phase response of NHBEs to cigarette smoke involves activation of both MAPK and NFκB.

  11. Untargeted Proteomics and Systems-Based Mechanistic Investigation of Artesunate in Human Bronchial Epithelial Cells.

    Science.gov (United States)

    Ravindra, Kodihalli C; Ho, Wanxing Eugene; Cheng, Chang; Godoy, Luiz C; Wishnok, John S; Ong, Choon Nam; Wong, W S Fred; Wogan, Gerald N; Tannenbaum, Steven R

    2015-10-19

    The antimalarial drug artesunate is a semisynthetic derivative of artemisinin, the principal active component of a medicinal plant Artemisia annua. It is hypothesized to attenuate allergic asthma via inhibition of multiple signaling pathways. We used a comprehensive approach to elucidate the mechanism of action of artesunate by designing a novel biotinylated dihydroartemisinin (BDHA) to identify cellular protein targets of this anti-inflammatory drug. By adopting an untargeted proteomics approach, we demonstrated that artesunate may exert its protective anti-inflammatory effects via direct interaction with multiple proteins, most importantly with a number of mitochondrial enzymes related to glucose and energy metabolism, along with mRNA and gene expression, ribosomal regulation, stress responses, and structural proteins. In addition, the modulatory effects of artesunate on various cellular transcription factors were investigated using a transcription factor array, which revealed that artesunate can simultaneously modulate multiple nuclear transcription factors related to several major pro- and anti-inflammatory signaling cascades in human bronchial epithelial cells. Artesunate significantly enhanced nuclear levels of nuclear factor erythroid-2-related factor 2 (Nrf2), a key promoter of antioxidant mechanisms, which is inhibited by the Kelch-like ECH-associated protein 1 (Keap1). Our results demonstrate that, like other electrophilic Nrf2 regulators, artesunate activates this system via direct molecular interaction/modification of Keap1, freeing Nrf2 for transcriptional activity. Altogether, the molecular interactions and modulation of nuclear transcription factors provide invaluable insights into the broad pharmacological actions of artesunate in inflammatory lung diseases and related inflammatory disorders. PMID:26340163

  12. Evaluations of thyme extract effects in human normal bronchial and tracheal epithelial cell lines and in human lung cancer cell line.

    Science.gov (United States)

    Oliviero, Marinelli; Romilde, Iannarelli; Beatrice, Morelli Maria; Matteo, Valisi; Giovanna, Nicotra; Consuelo, Amantini; Claudio, Cardinali; Giorgio, Santoni; Filippo, Maggi; Massimo, Nabissi

    2016-08-25

    Thyme (Thymus vulgaris) is used traditionally to prepare herbal remedies possessing expectorant, mucolytic, antitussive and antispasmodic properties. The aim of the present study was to investigate the effects of a standardized hydroalcoholic extract of thyme on primary human airway (bronchial/tracheal) epithelial cell lines in a model of lung inflammation induced by LPS. In addition, the effects of thyme extract on human lung cancer cell line (H460) were analysed. Thyme extract showed significant anti-inflammatory properties by reducing the NF-κB p65 and NF-κB p52 transcription factors protein levels followed by the decrease of pro-inflammatory cytokines (IL-1 beta and IL-8), and Muc5ac secretion in human normal bronchial and tracheal epithelial cells. Moreover, the extract showed cytotoxic effects on H460 cancer cells, modulated the release of IL-1 beta, IL-8 and down-regulated NF-κB p65 and NF-κB p52 proteins. Taken together, these results substantiated the traditional uses of thyme in the treatment of respiratory diseases. Thyme extract might be an effective treatment of chronic diseases based on inflammatory processes when hypersecretion of mucus overwhelms the ciliary clearance and obstructs airways, causing morbidity and mortality. Moreover thyme extract, evaluated in H460 lung cancer cell line, demonstrated to induce cell cytotoxicity in addition to reduce inflammatory cell signals. PMID:27369807

  13. Left bronchial artery arising from a replaced left hepatic artery in a patient with massive hemoptysis

    Energy Technology Data Exchange (ETDEWEB)

    Khil, Eun Kyung; Lee, Jae Myung [Dept. of Radiology, Soonchunhyang University College of Medicine, Bucheon Hospital, Bucheon (Korea, Republic of)

    2015-09-15

    A 70-year-old man with a 3-year history of bronchiectasis presented with massive hemoptysis that had lasted for 3 days. In our attempt to perform bronchial artery embolization, upper abdominal angiography was required to locate the left bronchial artery, which in this case was of anomalous origin, arising from a replaced left hepatic artery, which arose from the left gastric artery-a very unusual anatomical variant. We performed embolization with polyvinyl alcohol particles, and the patient's symptoms resolved completely, with no additional complications after conservative treatment.

  14. Cushing's like syndrome in typical bronchial carcinoid a case report and review of the literature.

    Science.gov (United States)

    Pedicelli, Ilaria; Patriciello, Giuseppina; Scala, Giovanni; Sorrentino, Antonietta; Gravino, Gennaro; Patriciello, Pasquale; Zeppa, Pio; Di Crescenzo, Vincenzo; Vatrella, Alessandro

    2016-01-01

    Cushing's syndrome occurred in 1-5% of cases of bronchial carcinoids. In this paper we describe a case of typical bronchial carcinoid in a nonsmoker young male with clinical manifestations mimicking a Cushing's syndrome. The patient performed chest radiograph and computed tomography. Fiberoptic bronchoscopy revealed the presence of an endobronchial mass occluding the bronchus intermedius. A rigid bronchoscopy was necessary for the conclusive diagnosis and for partial resection of the intraluminal tumor. Despite of the presence of Cushingoid features, the normal blood levels of ACTH and cortisol excluded the coexistence of a Cushing's syndrome.

  15. Congenital bronchial atresia coexistent with intralobar pulmonary sequestration: a case report

    Energy Technology Data Exchange (ETDEWEB)

    Han, Young Min; Ku, Ja Hong; Lee, Dong Keun; Chung, Kyung Ho; Kim, Chong Soo; Sohn, Myung Hee; Choi, Ki Chul [Chonbuk National University Medical School, Jeonju (Korea, Republic of)

    1995-02-15

    Bronchial atresia coexistent with intralobar pulmonary sequestration is so rare that only two cases have been reported in the literature. We report a case of congential bronchial atresia coexistent with intralobar pulmonary sequestation in a 51-year-old woman. Computed tomography showed the branching mass with hyperinflation of adjacent pulmonary parenchyma in the medial segment of the right middle lobe and a large thin-walled cystic mass with air-fluid levels in the medial basal segment of the right lower lobe. Selective inferior phrenic arteriography showed two aberrant arteries supplying the large cystic mass in the right lower lobe. The venous drainage was through the right pulmonary vein.

  16. Exercise-induced bronchoconstriction and non-specific airway hyperreactivity in patients suffering from bronchial asthma

    Directory of Open Access Journals (Sweden)

    Novković Dobrivoje

    2014-01-01

    Full Text Available Background/Aim. Physical activity is a common stimulus of asthmatic symptoms manifestation. Airway hyperreactivity is a predisposing cause of exercise induced bronchial obstruction, diagnosed by histamine inhalation. The aim of this study was to determine the relation between the amounts of histamine needed to induce non-specific airway hyperreactivity and exercise-induced bronchial obstruction. Methods. This randomized cross-over study included 160 male patients (age 19-27 years suffering from bronchial asthma who showed positive results as the reaction after the histamine bronchial provocation test. Histamine concentrations were in a range of 0.03 to 4 mg/mL. Each patient participated in the exercise stress test conducted on a conveyor belt. The results of the exercise stress test were considered positive if the FEV1 level dropped by at least 15% from its initial value, 5-10 minutes after the test. Results. All the patients showed positive results as the reaction after the histamine bronchial provocation test, while 50 of them showed positive results after the exercise-induced stress test. There was a statistically highly significant difference in administrated histamine concentrations between the group of patients that had positive results on exercise stress test and those who did not (1mg/mL vs 0.5mg/mL; U = 1678; p < 0.01. Also, there was a statistically significant difference concerning the frequency of the positive results regarding histamine concentration after induced stress test (χ2 = 10.885; p = 0.001. Among the patients with positive results, there was a statistically highly significant number of patients with bronchial obstruction induced by less than 2 mg/mL of histamine (p < 0.01. A statistically significant relation between the amount of histamine needed to induce bronchial obstruction and the results of the exercise stress test (p < 0.01 was also observed after the testing. Conclusion. In the group of patients with positive

  17. Proinflammatory cytokine responses induced by influenza A (H5N1 viruses in primary human alveolar and bronchial epithelial cells

    Directory of Open Access Journals (Sweden)

    Poon LLM

    2005-11-01

    Full Text Available Abstract Background Fatal human respiratory disease associated with influenza A subtype H5N1 has been documented in Hong Kong, and more recently in Vietnam, Thailand and Cambodia. We previously demonstrated that patients with H5N1 disease had unusually high serum levels of IP-10 (interferon-gamma-inducible protein-10. Furthermore, when compared with human influenza virus subtype H1N1, the H5N1 viruses in 1997 (A/Hong Kong/483/97 (H5N1/97 were more potent inducers of pro-inflammatory cytokines (e.g. tumor necrosis factor-a and chemokines (e.g. IP-10 from primary human macrophages in vitro, which suggests that cytokines dysregulation may play a role in pathogenesis of H5N1 disease. Since respiratory epithelial cells are the primary target cell for replication of influenza viruses, it is pertinent to investigate the cytokine induction profile of H5N1 viruses in these cells. Methods We used quantitative RT-PCR and ELISA to compare the profile of cytokine and chemokine gene expression induced by H5N1 viruses A/HK/483/97 (H5N1/97, A/Vietnam/1194/04 and A/Vietnam/3046/04 (both H5N1/04 with that of human H1N1 virus in human primary alveolar and bronchial epithelial cells in vitro. Results We demonstrated that in comparison to human H1N1 viruses, H5N1/97 and H5N1/04 viruses were more potent inducers of IP-10, interferon beta, RANTES (regulated on activation, normal T cell expressed and secreted and interleukin 6 (IL-6 in primary human alveolar and bronchial epithelial cells in vitro. Recent H5N1 viruses from Vietnam (H5N1/04 appeared to be even more potent at inducing IP-10 than H5N1/97 virus. Conclusion The H5N1/97 and H5N1/04 subtype influenza A viruses are more potent inducers of proinflammatory cytokines and chemokines in primary human respiratory epithelial cells than subtype H1N1 virus. We suggest that this hyper-induction of cytokines may be relevant to the pathogenesis of human H5N1 disease.

  18. 热休克蛋白与支气管哮喘%Heat shock proteins and bronchial asthma

    Institute of Scientific and Technical Information of China (English)

    马礼兵; 向旭东

    2011-01-01

    Heat shock proteins are a series of widespread proteins due to heat stress from bacteria to human, which are broadly participate in the process of immune regulation. Bronchial asthma (asthma)is a chronic airway inflammatory disease, which is also considered an autoimmune disease. With asthmatic incidence increased year by year, it causes a global common concern and become a common disease that is seriously danger to public health. Nowadays, the cellular immune dysfunction is considered important to the pathogenesis of asthma. There is growing evidence that heat shock proteins are likely involved in the pathogenesis of asthma.%热休克蛋白是从细菌到人类均广泛存在的一类热应急蛋白质,并且广泛参与了机体的免疫调节过程.支气管哮喘(简称哮喘)是一种气道慢性炎症性疾病,亦被认为是一种自身免疫性疾病,其发病率逐年上升,已成为全球普遍关注、严重危害公众健康的常见病.目前,细胞免疫功能紊乱被视为哮喘的重要发病机制.越来越多的证据表明,热休克蛋白极可能参与哮喘的发病过程.

  19. Value of past clinical history in differentiating bronchial asthma from COPD in male smokers presenting with SOB and fixed airway obstruction

    Directory of Open Access Journals (Sweden)

    Prahlad Rai Gupta

    2015-01-01

    Full Text Available Objective: Differentiating asthma from chronic obstructive pulmonary disease (COPD is difficult. Steroid trial may be of help but has several pitfalls. The present study aims to assess the value of past clinical profile of asthma and its differential diagnosis from COPD in male smokers and thereby to formulate clinical parameters to diagnose bronchial asthma in such patients. Patients and Methods: Male smokers who reported at the Respiratory Medicine Department of the National Institute of Medical Sciences (NIMS Hospital, Jaipur, (India, with shortness of breath (SOB and showing less than 12% postbronchodilator bronchial reversibility (BR on spirometry were recruited. These patients were given oral prednisolone 1 mg/kg for two weeks. Post steroid (PS spirometry was performed to ascertain BR. The past clinical history was recorded and analyzed to determine if it is of any use in differentiating asthma from COPD. Result: Out of 104 patients, four were lost to follow up, 52 were diagnosed as bronchial asthma, and the remaining 48 as COPD. It was revealed that past history of (H/O seasonal variation, wheezing, eye allergy, nasal allergy, dust allergy, skin allergy, and family H/O asthma/allergy were positive in 50, 40, 34, 30, 18, 14, and 12 asthma patients as compared to 10, 8, 2, 4, 6, 0, and 0 in 48 COPD patients (P < 0.001. The odds ratio (OR for diagnosing asthma was highest for the presence of any other two symptoms/variables, besides SOB, in the past (OR = 275, P < 0.0001. Conclusion: Past clinical history is of immense value in differentiating asthma from COPD in male smokers presenting with SOB and fixed airway obstruction.

  20. Retrospective Cohort Study of Bronchial Doses and Radiation-Induced Atelectasis After Stereotactic Body Radiation Therapy of Lung Tumors Located Close to the Bronchial Tree

    Energy Technology Data Exchange (ETDEWEB)

    Karlsson, Kristin, E-mail: kristin.karlsson@karolinska.se [Department of Medical Physics, Karolinska University Hospital, Stockholm (Sweden); Department of Oncology-Pathology, Karolinska Institute, Stockholm (Sweden); Nyman, Jan [Department of Oncology, Sahlgrenska University Hospital, Gothenburg (Sweden); Baumann, Pia; Wersäll, Peter [Department of Oncology, Radiumhemmet, Karolinska University Hospital, Stockholm (Sweden); Department of Oncology-Pathology, Karolinska Institute, Stockholm (Sweden); Drugge, Ninni [Department of Radiation Physics, Sahlgrenska University Hospital, Gothenburg (Sweden); Gagliardi, Giovanna [Department of Medical Physics, Karolinska University Hospital, Stockholm (Sweden); Department of Oncology-Pathology, Karolinska Institute, Stockholm (Sweden); Johansson, Karl-Axel [Department of Radiation Physics, Sahlgrenska University Hospital, Gothenburg (Sweden); Persson, Jan-Olov [Statistical Research Group, Mathematical Statistics, Stockholm University, Stockholm (Sweden); Rutkowska, Eva [Physics Department, Clatterbridge Cancer Centre, Wirral (United Kingdom); Tullgren, Owe [Department of Oncology, Radiumhemmet, Karolinska University Hospital, Stockholm (Sweden); Department of Oncology-Pathology, Karolinska Institute, Stockholm (Sweden); Lax, Ingmar [Department of Medical Physics, Karolinska University Hospital, Stockholm (Sweden); Department of Oncology-Pathology, Karolinska Institute, Stockholm (Sweden)

    2013-11-01

    Purpose: To evaluate the dose–response relationship between radiation-induced atelectasis after stereotactic body radiation therapy (SBRT) and bronchial dose. Methods and Materials: Seventy-four patients treated with SBRT for tumors close to main, lobar, or segmental bronchi were selected. The association between incidence of atelectasis and bronchial dose parameters (maximum point-dose and minimum dose to the high-dose bronchial volume [ranging from 0.1 cm{sup 3} up to 2.0 cm{sup 3}]) was statistically evaluated with survival analysis models. Results: Prescribed doses varied between 4 and 20 Gy per fraction in 2-5 fractions. Eighteen patients (24.3%) developed atelectasis considered to be radiation-induced. Statistical analysis showed a significant correlation between the incidence of radiation-induced atelectasis and minimum dose to the high-dose bronchial volumes, of which 0.1 cm{sup 3} (D{sub 0.1cm3}) was used for further analysis. The median value of D{sub 0.1cm3} (α/β = 3 Gy) was EQD{sub 2,LQ} = 147 Gy{sub 3} (range, 20-293 Gy{sub 3}). For patients who developed atelectasis the median value was EQD{sub 2,LQ} = 210 Gy{sub 3}, and for patients who did not develop atelectasis, EQD{sub 2,LQ} = 105 Gy{sub 3}. Median time from treatment to development of atelectasis was 8.0 months (range, 1.1-30.1 months). Conclusion: In this retrospective study a significant dose–response relationship between the incidence of atelectasis and the dose to the high-dose volume of the bronchi is shown.

  1. HDR intralumenal brachytherapy in bronchial cancer: review of our experience

    International Nuclear Information System (INIS)

    The main indications for brachytherapy in the treatment of endobronchial cancer are dyspnea. postobstructive pneumonia and atelectasis, cough and hemoptysis resulting from broncus obstruction by exophytic intralumenal tumor growth. High Dose Rate intralumenal brachytherapy (HDRBT) may be combined with external beam radiotherapy (EBRT), in particular as almost all tumors are too large for HDRBT alone. From January 1992 to September 1995 we treated 268 patients affected by bronchial cancer, with EBRT combined with HDRBT. All patients were staged as IIIa-IIIb-IV but KPS was >60 and expectancy of life > than 3 months. After bronchoscopy and Tc simulation we found that almost 10% of patients were downstaged. Treatment was always realized delivering 60 Gy to the tumour volume and 50 Gy to the mediastinal structures with EBRT. Brachytherapy was performed during the radiotherapy course. In 38 patients HDRBT was realized just one time, at the beginning of EBRT, with a dose of 10 Gy calculated at 1cm from the central axis of the catheter. In 47 HDRBT was performed twice (at the beginning and at the end of EBRT) with a dose of 7 Gy calculated at 1 cm from the central axis. From 1994 we started a 3 fractions protocol (Timing: days 1.15.30) with a dose of 5 Gy calculated at 0.5 cm from the axis. Of the 183 patients introduced in the protocol 170 received the three fractions of HDRBT and 13 were excluded from the study for personal or clinical reasons. In 97% of cases the application did not need general anesthesia; local anesthesia has been sufficient supplemented by some drug for sedation and coughing. Anyway both bronchoscopy and HDRBT (with anterior-posterior and lateral chest X-ray) are performed in the same shielded room without the necessity of displacing the patient. In almost 60% of treatments we used just one endobronchial applicator. In case of tumor involvement of the carina, two applicators were introduced. By this a larger tumor volume can be treated with adequate

  2. Analysis of related factors of bronchial asthma after bronchiolitis%毛细支气管炎后支气管哮喘发生的相关因素分析

    Institute of Scientific and Technical Information of China (English)

    廖先辉

    2015-01-01

    Objective To explore the related factors of bronchial asthma after bronchiolitis.MethodsClinical data of 66 children with bronchiolitis who were admitted to our hospital from January,2007 to January,2008 were analyzed retrospectively and the conditions of respiratory tract infection and bronchial asthma of these children after discharge were analyzed.Factors that may cause bronchial asthma and correlations between these factors and bronchial asthma attacks were analyzed in Logistic regression analysis method according to conditions of bronchial asthma of these children.Children were given 5-year follow-ups after discharge,those with bronchial asthma were allocated as the disease group while those without bronchial asthma were allocated to the normal group. Results Children were given 5-year follow-ups after discharge,among whom 25 were with bronchial asthma and the rate was 37.88%.The family history of asthma,lower respiratory tract infection,age and condition of bronchiolitis,etc.all of children in two groups were found to have significant differences after various factors in two groups were compared.These differences had statistically significance(P<0.05).ConclusionSevere conditions,the family history of asthma, accompanied with lower respiratory tract infection etc.all of children with bronchiolitis were all main factors of bronchial asthma caused by bronchiolitis.%目的:探讨毛细支气管炎后与支气管哮喘发生的相关因素。方法对我院2007年1月~2008年1月期间收治的66例毛细支气管炎患儿的临床资料进行回顾性分析,在患儿出院后对其呼吸道感染与气管哮喘发作情况进行分析,并根据患儿的支气管哮喘发生情况采用Logistic回归分析法对可能引发支气管哮喘的因素及这些因素和支气管哮喘发病之前的相关性进行分析。患儿出院给予5年随访,发生支气管哮喘者归为发病组,而未发病者归为正常组。结果患儿出院给予5

  3. Repeatability of and relationship between potential COPD biomarkers in bronchoalveolar lavage, bronchial biopsies, serum, and induced sputum.

    Directory of Open Access Journals (Sweden)

    Stefan Röpcke

    Full Text Available Chronic Obstructive Pulmonary Disease (COPD is a chronic inflammatory disease, primarily affecting the airways. Stable biomarkers characterizing the inflammatory phenotype of the disease, relevant for disease activity and suited to predict disease progression are needed to monitor the efficacy and safety of drug interventions. We therefore analyzed a large panel of markers in bronchoalveolar lavage, bronchial biopsies, serum and induced sputum of 23 healthy smokers and 24 smoking COPD patients (GOLD II matched for age and gender. Sample collection was performed twice within a period of 6 weeks. Assays for over 100 different markers were validated for the respective matrices prior to analysis. In our study, we found 51 markers with a sufficient repeatability (intraclass correlation coefficient >0.6, most of these in serum. Differences between groups were observed for markers from all compartments, which extends (von-Willebrand-factor and confirms (e.g. C-reactive-protein, interleukin-6 previous findings. No correlations between lung and serum markers were observed, including A1AT. Airway inflammation defined by sputum neutrophils showed only a moderate repeatability. This could be improved, when a combination of neutrophils and four sputum fluid phase markers was used to define the inflammatory phenotype.In summary, our study provides comprehensive information on the repeatability and interrelationship of pulmonary and systemic COPD-related markers. These results are relevant for ongoing large clinical trials and future COPD research. While serum markers can discriminate between smokers with and without COPD, they do not seem to sufficiently reflect the disease-associated inflammatory processes within the airways.

  4. HIV gp120 induces mucus formation in human bronchial epithelial cells through CXCR4/α7-nicotinic acetylcholine receptors.

    Directory of Open Access Journals (Sweden)

    Sravanthi Gundavarapu

    Full Text Available Lung diseases such as chronic obstructive pulmonary disease (COPD, asthma, and lung infections are major causes of morbidity and mortality among HIV-infected patients even in the era of antiretroviral therapy (ART. Many of these diseases are strongly associated with smoking and smoking is more common among HIV-infected than uninfected people; however, HIV is an independent risk factor for chronic bronchitis, COPD, and asthma. The mechanism by which HIV promotes these diseases is unclear. Excessive airway mucus formation is a characteristic of these diseases and contributes to airway obstruction and lung infections. HIV gp120 plays a critical role in several HIV-related pathologies and we investigated whether HIV gp120 promoted airway mucus formation in normal human bronchial epithelial (NHBE cells. We found that NHBE cells expressed the HIV-coreceptor CXCR4 but not CCR5 and produced mucus in response to CXCR4-tropic gp120. The gp120-induced mucus formation was blocked by the inhibitors of CXCR4, α7-nicotinic acetylcholine receptor (α7-nAChR, and γ-aminobutyric acid (GABAAR but not the antagonists of CCR5 and epithelial growth factor receptor (EGFR. These results identify two distinct pathways (α7-nAChR-GABAAR and EGFR for airway mucus formation and demonstrate for the first time that HIV-gp120 induces and regulates mucus formation in the airway epithelial cells through the CXCR4-α7-nAChR-GABAAR pathway. Interestingly, lung sections from HIV ± ART and simian immunodeficiency virus (SIV ± ART have significantly more mucus and gp120-immunoreactivity than control lung sections from humans and macaques, respectively. Thus, even after ART, lungs from HIV-infected patients contain significant amounts of gp120 and mucus that may contribute to the higher incidence of obstructive pulmonary diseases in this population.

  5. (Endo)cannabinoid signaling in human bronchial epithelial and smooth muscle cells

    NARCIS (Netherlands)

    Gkoumassi, Effimia

    2007-01-01

    We investigated the pathways used by various (endo)cannabinoids in regulating intracellular calcium homeostasis, adenylyl cyclase and ERK signaling, in bronchial epithelial cells as well as smooth muscle cells. In DDT1 MF2 smooth muscle cells the synthetic cannabinoid CP55,940 increases [Ca2+]i by a

  6. Cavitary lung cancer lined with normal bronchial epithelium and cancer cells.

    Science.gov (United States)

    Goto, Taichiro; Maeshima, Arafumi; Oyamada, Yoshitaka; Kato, Ryoichi

    2011-01-01

    Reports of cavitary lung cancer are not uncommon, and the cavity generally contains either dilated bronchi or cancer cells. Recently, we encountered a surgical case of cavitary lung cancer whose cavity tended to enlarge during long-term follow-up, and was found to be lined with normal bronchial epithelium and adenocarcinoma cells. PMID:21980325

  7. Cavitary Lung Cancer Lined with Normal Bronchial Epithelium and Cancer Cells

    OpenAIRE

    Goto, Taichiro; Maeshima, Arafumi; Oyamada, Yoshitaka; Kato, Ryoichi

    2011-01-01

    Reports of cavitary lung cancer are not uncommon, and the cavity generally contains either dilated bronchi or cancer cells. Recently, we encountered a surgical case of cavitary lung cancer whose cavity tended to enlarge during long-term follow-up, and was found to be lined with normal bronchial epithelium and adenocarcinoma cells.

  8. Cavitary Lung Cancer Lined with Normal Bronchial Epithelium and Cancer Cells

    Directory of Open Access Journals (Sweden)

    Taichiro Goto, Arafumi Maeshima, Yoshitaka Oyamada, Ryoichi Kato

    2011-01-01

    Full Text Available Reports of cavitary lung cancer are not uncommon, and the cavity generally contains either dilated bronchi or cancer cells. Recently, we encountered a surgical case of cavitary lung cancer whose cavity tended to enlarge during long-term follow-up, and was found to be lined with normal bronchial epithelium and adenocarcinoma cells.

  9. [Circadian organization of the indices of external respiration in bronchial asthma patients].

    Science.gov (United States)

    Fedoseev, G B; Degtiareva, Z Ia; Savich, A A; Alekseev, M Iu; Bolodon, S A

    1985-01-01

    Circadian rhythms of external respiration function (ERF) and body function (BF) were studied in 20 patients with mild bronchial asthma, 20 patients with bronchial asthma of medium gravity and in 14 healthy subjects. Circadian rhythms of the medium-group parameters of ERF were shown to be statistically insignificant, whereas individual circadian chronograms demonstrated circadian rhythms for all the patients with BA and healthy subjects. Essential differences were found in the organization of the temporary structure of circadian rhythms of ERF in BA patients and healthy subjects. It was discovered that in BA patients, the maximal pathological alterations develop within the period from the midnight to 8 a. m., whereas the minimal disorders of ERF are recorded within the period from the noon to 8 p. m. It is suggested that deterioration of bronchial patency which brings about the development of obstruction in BA patients is the result of hypersynchronization and increased amplitude of the circadian rhythms of those parameters of ERF which determine the bronchial patency. PMID:4002163

  10. Clinical Observation on Prevention of Bronchial Asthma with Plaster on Acupoints

    Institute of Scientific and Technical Information of China (English)

    YU Jin-long; ZHU Han-ting

    2008-01-01

    @@ Bronchial asthma is a most common and chronicillness which often relapses and has no cure at present.Acupuncture has its specific advantage in treatingasthma. The author prevented 29 cases of asthma innon-acute stage of attack with plaster on acupoints.Now it is reported as follows.

  11. Bronchial thermoplasty: a new therapeutic option for the treatment of severe, uncontrolled asthma in adults.

    Science.gov (United States)

    Dombret, Marie-Christine; Alagha, Khuder; Boulet, Louis Philippe; Brillet, Pierre Yves; Joos, Guy; Laviolette, Michel; Louis, Renaud; Rochat, Thierry; Soccal, Paola; Aubier, Michel; Chanez, Pascal

    2014-12-01

    Bronchial thermoplasty is a young yet promising treatment for severe asthma whose benefit for long-term asthma control outweighs the short-term risk of deterioration and hospitalisation in the days following the treatment. It is an innovative treatment whose clinical efficacy and safety are beginning to be better understood. Since this is a device-based therapy, the overall evaluation of risk-benefit is unlike that of pharmaceutical products; safety aspects, regulatory requirements, study design and effect size assessment may be unfamiliar. The mechanisms of action and optimal patient selection need to be addressed in further rigorous clinical and scientific studies. Bronchial thermoplasty fits in perfectly with the movement to expand personalised medicine in the field of chronic airway disorders. This is a device-based complimentary asthma treatment that must be supported and developed in order to meet the unmet needs of modern severe asthma management. The mechanisms of action and the type of patients that benefit from bronchial thermoplasty are the most important challenges for bronchial thermoplasty in the future. PMID:25445950

  12. Bronchial thermoplasty: a new therapeutic option for the treatment of severe, uncontrolled asthma in adults

    Directory of Open Access Journals (Sweden)

    Marie-Christine Dombret

    2014-12-01

    Full Text Available Bronchial thermoplasty is a young yet promising treatment for severe asthma whose benefit for long-term asthma control outweighs the short-term risk of deterioration and hospitalisation in the days following the treatment. It is an innovative treatment whose clinical efficacy and safety are beginning to be better understood. Since this is a device-based therapy, the overall evaluation of risk–benefit is unlike that of pharmaceutical products; safety aspects, regulatory requirements, study design and effect size assessment may be unfamiliar. The mechanisms of action and optimal patient selection need to be addressed in further rigorous clinical and scientific studies. Bronchial thermoplasty fits in perfectly with the movement to expand personalised medicine in the field of chronic airway disorders. This is a device-based complimentary asthma treatment that must be supported and developed in order to meet the unmet needs of modern severe asthma management. The mechanisms of action and the type of patients that benefit from bronchial thermoplasty are the most important challenges for bronchial thermoplasty in the future.

  13. Cellular response of mucociliary differentiated primary bronchial epithelial cells to diesel exhaust

    NARCIS (Netherlands)

    Zarcone, M.C.; Duistermaat, E.; Schadewijk, A. van; Jedynksa, A.D.; Hiemstra, P.S.; Kooter, I.M.

    2016-01-01

    Cellular response of mucociliary differentiated primary bronchial epithelial cells to diesel exhaust. Am J Physiol Lung Cell Mol Physiol 311: L111–L123, 2016. First published May 17, 2016; doi:10.1152/ajplung.00064.2016.—Diesel emissions are the main source of air pollution in urban areas, and diese

  14. Dynamics of eosinophil infiltration in the bronchial mucosa before and after the late asthmatic reaction.

    Science.gov (United States)

    Aalbers, R; de Monchy, J G; Kauffman, H F; Smith, M; Hoekstra, Y; Vrugt, B; Timens, W

    1993-06-01

    We wanted to determine whether changes in bronchial hyperresponsiveness (BHR) following allergen challenge show a time relationship with inflammatory events in the airways of allergic asthmatic subjects. Lavage was performed and endobronchial biopsies were taken via the fiberoptic bronchoscope, before, and 3 and 24 h after, allergen challenge, on separate occasions, in nine dual asthmatic responders. The numbers of activated eosinophils, identified by immunohistochemistry, using the monoclonal anti-eosinophil cationic protein antibody, EG2, were significantly increased both at 3 h and at 24 h in the submucosa and bronchial lavage. A significant negative correlation was found between the number of EG2+ cells in the submucosa and in the bronchial lavage 24 h after the allergen challenge (r = -0.70). At 24 h, the amount of eosinophil cationic protein (ECP) was increased in the bronchial lavage. A significant correlation was observed between the amount of ECP at 3 h and the log provocative dose of house dust mite producing a 20% fall in forced expiratory volume in one second (PD20 HDM) (r = -0.63). The results suggest a recruitment of activated eosinophils to the submucosa and, further, to the epithelial lining, followed by degranulation. This process has already started 3 h after allergen challenge, and lasts for at least 24 h, which may result in mucosal damage and subsequent allergen-induced increase in BHR, before and after the late asthmatic reaction. PMID:8339804

  15. Bronchial thermoplasty : Long-term safety and effectiveness in patients with severe persistent asthma

    NARCIS (Netherlands)

    Wechsler, Michael E.; Laviolette, Michel; Rubin, Adalberto S.; Fiterman, Jussara; Silva, Jose R. Lapa e; Shah, Pallav L.; Fiss, Elie; Olivenstein, Ronald; Thomson, Neil C.; Niven, Robert M.; Pavord, Ian D.; Simoff, Michael; Hales, Jeff B.; McEvoy, Charlene; Slebos, Dirk-Jan; Holmes, Mark; Phillips, Martin J.; Erzurum, Serpil C.; Hanania, Nicola A.; Sumino, Kaharu; Kraft, Monica; Cox, Gerard; Sterman, Daniel H.; Hogarth, Kyle; Kline, Joel N.; Mansur, Adel H.; Louie, Brian E.; Leeds, William M.; Barbers, Richard G.; Austin, John H. M.; Shargill, Narinder S.; Quiring, John; Armstrong, Brian; Castro, Mario

    2013-01-01

    Background: Bronchial thermoplasty (BT) has previously been shown to improve asthma control out to 2 years in patients with severe persistent asthma. Objective: We sought to assess the effectiveness and safety of BT in asthmatic patients 5 years after therapy. Methods: BT-treated subjects from the A

  16. Epidemiologic approach to determining the REM dose to bronchial epithelium from radon daughter exposures in man

    International Nuclear Information System (INIS)

    Dosimetric evaluation of exposures to the bronchial epithelial stem cells from inhalation of alpha emitters is complex and the number of assumptions required leads to substantial uncertainty. Moreover, it is evident that many factors such as variable breathing patterns will modify the results. An empirical approach to determining the rem dose biologically effective in producing bronchial cancer by inhalation of radon daughters is possible by comparison of cumulative exposure in terms of Working Level Months with the dose of penetrating low-LET radiation to produce the same bronchial cancer risk in other groups investigated. This method is possible when the dosimetry of the comparison groups is well known and lung cancer risks are reasonably well-defined. A number of epidemiologic considerations must be taken into account in such comparisons, however. These include age at exposure, length of following, age at expression of or death from cancer, and for underground miners exposed to radon daughters the lung cancer-latent period model applied to long-term exposures. Use of these methods gives results consistent with dosimetry derived from models of bronchial deposition and clearance, as well as reasonable values for the quality factor for alpha radiation. This comparison gives an average equivalent dose for a working population exposed to short-lived isotopes

  17. Bronchial histamine challenge. A combined interrupter-dosimeter method compared with a standard method

    DEFF Research Database (Denmark)

    Pavlovic, M; Holstein-Rathlou, N H; Madsen, F;

    1985-01-01

    We compared the provocative concentration (PC) values obtained by two different methods of performing bronchial histamine challenge. One test was done on an APTA, an apparatus which allows simultaneous provocation with histamine and measurement of airway resistance (Rtot) by the interrupter metho...

  18. Bronchography in patients with the infections-allergic form of bronchial asthma

    International Nuclear Information System (INIS)

    Bronchography has been performed on 154 patients, having the infectious-allergic form of bronchial asthma. Pathologic changes on the bronchograms have been detected in 99 (64.3 %) patients. Deforming bronchitis, characterized by the different degree of manifestness and stretch, has been more frequent (49.3%), while bronchoectases (14.3%) and cirrhosis (0.7%) have been rarer findings

  19. On the purported discovery of the bronchial circulation by Leonardo da Vinci.

    Science.gov (United States)

    Mitzner, W; Wagner, E

    1992-09-01

    Among modern physiologists and anatomists, there has been a nearly universal acceptance that Leonardo da Vinci was the first to identify the anatomy of the bronchial circulation. However, because of certain ambiguities in both his anatomic drawing that was supposed to have shown this circulation and the accompanying descriptive text, we questioned whether he really could have been the first to discover this small but important vasculature. To address this question, we set out to repeat Leonardo's dissections in the ox. We reasoned that perhaps the normally tiny bronchial vessels would be considerably more noticeable in this very large species. Our dissections, however, failed to provide any evidence that Leonardo's drawing was that of the bronchial circulation. Furthermore we observed a set of distinct small pulmonary veins to the left upper and right middle lobes that Leonardo, given his lack of understanding of the function of the lung and its circulation, could have easily mistaken for a separate circulation. We thus conclude that Leonardo da Vinci did not describe the anatomy of the bronchial circulation. We believe that the first person to clearly and unequivocally describe the anatomy of this circulation was the Dutch Professor of Anatomy and Botany, Frederich Ruysch.

  20. Posterior Circulation Stroke After Bronchial Artery Embolization. A Rare but Serious Complication

    Energy Technology Data Exchange (ETDEWEB)

    Laborda, Alicia [Universidad de Zaragoza, Departamento de Pediatria, Radiologia y Medicina Fisica, Grupo de Investigacion en Tecnicas Minimamente Invasivas, GITMI (Spain); Tejero, Carlos [Hospital Clinico Universitario Lozano Blesa, Servicio de Neurologia (Spain); Fredes, Arturo, E-mail: fredesarturo@gmail.com [Universidad de Zaragoza, Hospital Quiron, Departamento de Pediatria, Radiologia y Medicina Fisica, Grupo de Investigacion en Tecnicas Minimamente Invasivas, GITMI (Spain); Cebrian, Luis; Guelbenzu, Santiago; Gregorio, Miguel Angel de, E-mail: mgregori@unizar.es [Universidad de Zaragoza, Departamento de Pediatria, Radiologia y Medicina Fisica, Grupo de Investigacion en Tecnicas Minimamente Invasivas, GITMI (Spain)

    2013-06-15

    Bronchial artery embolization (BAE) is the treatment of choice for massive hemoptysis with rare complications that generally are mild and transient. There are few references in the medical literature with acute cerebral embolization as a complication of BAE. We report a case of intracranial posterior territory infarctions as a complication BAE in a patient with hemoptysis due to bronchiectasis.

  1. Bronchial wall measurements in patients after lung transplantation: evaluation of the diagnostic value for the diagnosis of bronchiolitis obliterans syndrome.

    Directory of Open Access Journals (Sweden)

    Sabine Dettmer

    Full Text Available OBJECTIVES: To prospectively evaluate quantitative airway wall measurements of thin-section CT for the diagnosis of Bronchiolitis Obliterans Syndrome (BOS following lung transplantation. MATERIALS AND METHODS: In 141 CT examinations, bronchial wall thickness (WT, the wall area percentage (WA% calculated as the ratio of the bronchial wall area and the total area (sum of bronchial wall area and bronchial lumen area and the difference of the WT on inspiration and expiration (WTdiff were automatically measured in different bronchial generations. The measurements were correlated with the lung function parameters. WT and WA% in CT examinations of patients with (n = 25 and without (n = 116 BOS, were compared using the unpaired t-test and univariate analysis of variance, while also considering the differing lung volumes. RESULTS: Measurements could be performed in 2,978 bronchial generations. WT, WA%, and WTdiff did not correlate with the lung function parameters (r<0.5. The WA% on inspiration was significantly greater in patients with BOS than in patients without BOS, even when considering the dependency of the lung volume on the measurements. WT on inspiration and expiration and WA% on expiration did not show significant differences between the groups. CONCLUSION: WA% on inspiration was significantly greater in patients with than in those without BOS. However, WA% measurements were significantly dependent on lung volume and showed a high variability, thus not allowing the sole use of bronchial wall measurements to differentiate patients with from those without BOS.

  2. DISEASES

    DEFF Research Database (Denmark)

    Pletscher-Frankild, Sune; Pallejà, Albert; Tsafou, Kalliopi;

    2015-01-01

    Text mining is a flexible technology that can be applied to numerous different tasks in biology and medicine. We present a system for extracting disease-gene associations from biomedical abstracts. The system consists of a highly efficient dictionary-based tagger for named entity recognition...... of human genes and diseases, which we combine with a scoring scheme that takes into account co-occurrences both within and between sentences. We show that this approach is able to extract half of all manually curated associations with a false positive rate of only 0.16%. Nonetheless, text mining should...... not stand alone, but be combined with other types of evidence. For this reason, we have developed the DISEASES resource, which integrates the results from text mining with manually curated disease-gene associations, cancer mutation data, and genome-wide association studies from existing databases...

  3. Autonomic control of bronchial circulation in awake sheep during rest and behaviour.

    Science.gov (United States)

    McIlveen, S; White, S; Parsons, G

    1997-12-01

    1. We tested the hypothesis that the pattern and the intensity of autonomic mechanisms causing vasoconstriction in the resting bronchial circulation of awake dogs also exists in awake sheep. It was also postulated that sighing behaviour and the associated bronchovascular dilatation induced by non-adrenergic, non-cholinergic (NANC) mechanisms observed in the dog exist in sheep. 2. Bronchial arterial blood flow to lower airways of both lungs of awake sheep was measured continuously using pulsed Doppler flow probes mounted on the bronchial artery at prior thoracotomy. 3. Cumulative and factorial analysis of responses to randomized combinations of autonomic alpha 1-, alpha 2-, beta 1- and beta 2-adrenoceptors and cholinoceptor autonomic blockade suggests that resting vasoconstrictor activity is less in sheep than in dogs. At normal aortic pressure, the autonomic activity of these receptor groups in the sheep lowers bronchial blood flow and conductance by 30%, whereas in the awake dog, the corresponding autonomic effect is 50%. 4. Tonic autonomic control of bronchial conductance can be partitioned in sheep to show significant and separate alpha- and beta-adrenoceptor vasoconstrictor activity at a ratio of 1.8:1, an effect normally offset by a weaker vasodilator alpha-/beta-adrenoceptor interaction. In contrast to the situation in awake dogs, cholinoceptors do not play a role in awake sheep. 5. Nitric oxide (NO) synthase inhibition in sheep using NG-nitro-L-arginine following blockade of alpha- and beta-adrenoceptors and cholinoceptors causes hypertension, but minor changes, if any, in pulmonary pressures or heart rate. Bronchial flow and conductance, however, fall from a higher resting conductance by approximately 50%, suggesting that, normally, resting bronchial flow conductance is dominated by strong tonic NO vasodilator effects that interact with weaker tonic autonomic vasoconstrictor effects. 6. Superimposed (respiratory) behaviours of sighing, sneezing and coughing

  4. Rates and predictors of uncontrolled bronchial asthma in elderly patients from western Romania

    Directory of Open Access Journals (Sweden)

    Marincu I

    2015-06-01

    Full Text Available Iosif Marincu,1 Stefan Frent,1 Mirela Cleopatra Tomescu,2 Stefan Mihaicuta1 1Department of Infectious Diseases, Pulmonology, Epidemiology and Parasitology, 2Department of Internal Medicine I, Victor Babes University of Medicine and Pharmacy, Timisoara, Romania Purpose: Bronchial asthma (BA is a chronic inflammatory disorder of the airways, featuring variable and often reversible airflow limitations. An accurate assessment of BA control is difficult in practice, especially in the elderly, requiring the assessment of several clinical and paraclinical parameters that are influenced not only by asthma, but also by comorbidities. The purpose of this study was to evaluate the predictors of uncontrolled BA in a group of elderly patients from western Romania.Patients and methods: We retrospectively evaluated 126 elderly patients (aged ≥ 65 years, who were consecutively evaluated in the Pulmonology Department of Victor Babes Hospital, Timisoara, Romania, between March 2009 and July 2012. We collected demographic data, performed pulmonary function testing and an asthma control test (ACT, and evaluated the level of BA control based on the 2012 Global Initiative for Asthma guidelines. Statistical processing of the data was done using the Epi Info and STATA programs.Results: In our study group, 36 (29% patients were men and 90 (71% were women; their mean age was 74.42±8.32 years (range: 65–85 years. A total of 14.28% of patients were smokers. About 30.15% of patients had an ACT score <19, 54.76% had an ACT score 20–24, and 15.09% had an ACT of 25. Moreover, 59.52% had normal spirometry results. Infectious exacerbations were found in 58.73% of patients. A history of allergies was demonstrated in 48.41% of patients, 34.12% had occupational exposure, and 82.53% of patients were treated with inhaled corticosteroids. Our results showed that 30.15% of patients had uncontrolled BA. We found six predictive factors for uncontrolled BA: infectious exacerbation

  5. Clinical advances on Bronchial Asthma. Changing perceptions. Avances clínicos en asma bronquial

    Directory of Open Access Journals (Sweden)

    Beatriz Home López Viera

    2007-06-01

    Full Text Available Bronchial Asthma brepresents an important health problem worlwide due to the increment of its incidence in the last years. The general mortality is low in regard to morbidity but it could be potencially lethal. It is consider a frequent disease in our country when it is found in patients older than 15 years old and female sex is more prevalent than the male one. It has influence upon not only the ill person but also in the family ad society. The sanitary education is an important aspect for the correct control of sick patients. Within the following paper it is our intention to update the knowledge about this disease through a bibliographic revision. Epidemiology, physiopathology, classification, complementary explorations, as well as the factors that must be paid attention on its treatment are found among the processed aspects, all which will serve for consultation not only for students but also for professionals of the medical science branch.
    El asma bronquial representa un importante problema de salud en todo el mundo, pues su incidencia se ha incrementado en los últimos tiempos. La mortalidad general es baja en relación a la morbilidad, pero puede ser potencialmente mortal. En nuestro país se considera una enfermedad frecuente, posterior a los 15 años y hay predominio del sexo femenino. Repercute no sólo en el enfermo sino tambien en la familia y en la sociedad. La educación sanitaria es un aspecto importante para el correcto control de los enfermos. Con el presente trabajo se pretende actualizar los conocimientos acerca de esta enfermedad, a través de una revisión bibliográfica. Entre los aspectos tratados se encuentran: la epidemiología, la fisiopatología, la clasificación, las exploraciones complementarias, así como los factores que deben ser atendidos en su tratamiento, todo lo cual servirá para consulta de estudiantes y profesionales de las ciencias médicas.

  6. Bronchial Brushing Increases the Diagnostic Yield of Fiberoptic Bronchoscopy in Bronchogenic Carcinoma

    Directory of Open Access Journals (Sweden)

    Recep Bedir

    2014-07-01

    Full Text Available Background: The importance of rapid and accurate cytopathological diagnosis in bronchial cancers is increasing due to advances in treatment modalities.Aims: We evaluated the diagnostic methods and cytologic subtypes of bronchial cancers to determine the diagnostic reliability of different bronchoscopic techniques.Material and methods: Retrospective data were obtained from the hospital files and pathological specimens of the patients with diagnosis of primary lung cancer from a period of 36 months. Cytological tumor typing was determined using histopathology of bronchoscopic forceps biopsy (FB, bronchial-bronchoalveolar lavage (BL, bronchial brushing (BB, transbronchial fine-needle biopsy. Computed tomography or ultrasonography guided transthoracic biopsy and surgical biopsies were used where the other interventional methods were inadequate for diagnosis.Results: A total of 124 patients were diagnosed during study period. 119 (96% of them were male. The median age was 68, ranging between 36 and 88 years. Histopathologic subtypes were determined as non-small cell carcinoma (NSCC in 104 (83.9%, squamous cell carcinoma in 64 (51.6%, adenocarcinoma in 16 (12.9%, NSCC not otherwise specified in 24 (19.3% and small cell carcinoma in 20 (16.1% patients. The combination of FB, BL and BB established the diagnosis of bronchogenic carcinoma in most of the cases (92.6%.Conclusions: Lung cancer is seen commonly in elderly male patients with smoking history and squamous cell carcinoma is the most common cytologic type. High diagnostic accuracy can be achieved by a combination of bronchoscopic FB, BB and BL procedures. Keywords: Bronchoscopy; Lung cancer; Bronchial brushing

  7. Preliminary study on lung volume reduction by bronchial occlusion with pingyangmycin-lipiodol emulsion

    International Nuclear Information System (INIS)

    Objective: To evaluate the feasibility and safety of lung volume reduction by trans-catheter bronchial occlusion with Pingyangmycin-lipiodol emulsion (PLE) followed by occlusion of the selective proximal segmental bronchial lumen with bone cement. Methods: After selective segmental bronchial catheterization in 12 adult rabbits, occlusion with PLE was performed. The animals were divided into 3 treatment groups (n=4 in each group) and 4 mg, 8 mg and 16 mg of Pingyangmycin mixed with 1 ml lipiodol were used respectively. For control , occlusion with pure lipiodol 1 ml was carried out in another 2 rabbits. After all bone cement 0.5 ml was added to occlude the selective proximal segmental bronchial lumen in all animals. Followed up procedures were carried out with imaging examination at 1, 2, 4 week after the occlusion procedures in all animals, and finally the whole lungs were harvested for histological examination. Results: The findings in pure lipiodol group were similar with those in PLE group. Significant atelectasis was observed at occluded segments, with filling of lipiodol and contraction of lung gradually on chest X-films and CT images. Pathologically, the lesions of target areas showed acute alveolitis in the initial stage, mild pulmonary fibrosis surrounded by collapsing pulmonary alveoli with interstitial imfiltration of eosinophile granulocytes and lymphocytes along with a lot of aggregating pulmonary macrophages together with small necrosis or abscess formation in partial target areas(6/14) after 4 weeks of the procedure. Macroscopically, visceral pleura of target areas, remained intact without pleural effusion or adhesion. Pneumonia was not happened in the non-target areas. Conclusions: Transbronchial occlusion with PLE or pure lipiodol followed by occlusion of the selective proximal segmental bronchial lumen with bone cement is safe and effective. No significant difference was shown between the extents of pulmonary fibrosis between the two groups

  8. Disorders of cardiac hemodynamic in attack period of bronchial asthma in children

    Directory of Open Access Journals (Sweden)

    Kondratiev V.А.

    2016-05-01

    Full Text Available By dopplerechocardiography method there was studied functional state of cardiac ventricles and character of hemodynamic disorders in 48 patients aged 5-17 years in attack period of moderately-severe and severe bronchial asthma. Group of comparison included 40 healthy peers. Disorders of central and peripheral hemodynamic in attack period of bronchial asthma in children were accompanied both by systolic and diastolic dysfunction of the left and right heart ventricles, herewith right ventricle was functioning in the mode of hyperdynamic, and left one – in the mode of hypodynamic. Combined systolic-diastolic variant of dysfunction both of right and left ventricles was developing in 58,3% of patients with moderately-severe and in 91,6% of patients with severe bronchial asthma. In the attack period of bronchial asthma in children equal directionality of systolic and diastolic dysfunction of heart ventricles was developing; this was characterized by synchronization of their function. Assessment of functional interaction of the ventricles under conditions of severe asthma attack showed direct and high (r=0,67 correlative interaction between finding of Tei index of the left and right ventricles, which characterize their systolic function; this, under conditions of increased hemodynamic pre-loading testified to compensatory increase of systolic interaction of ventricles. Direct and high (r=0,69 correlative interaction between time indices of isovolumic relaxation of the left and right ventricles, characterizing their diastolic function, testified to compensatory increase of diastolic interaction of ventricles under conditions of increase of hemodynamic post-loading. Imbalance of central and peripheral link of hemodynamic in attack period of bronchial asthma in children testified to development of cardiac insufficiency, which was compensated predominantly at the expense of increase of heart contractions rate.

  9. Toxocara canis y asma bronquial Toxocara canis and bronchial asthma

    Directory of Open Access Journals (Sweden)

    María de los Ángeles López

    2010-02-01

    symptoms of grade I there was a 37.5% (p = 0.0470. The infection with T. canis could act as a co-factor increasing the severity of the symptoms of bronchial asthma.

  10. Clinical significance of changes of serum TNF-α and IL-6 levels in elderly patients with chronic bronchial asthma

    International Nuclear Information System (INIS)

    Objective: To explore the clinical significance of changes of serum TNF-α and IL-6 levels in elderly patients with chronic bronchial asthma. Methods: Serum TNF-α and IL-6 levels were determined with RIA in 55 elderly patients with chronic bronchial asthma and 35 controls. Results: Serum TNF-α and IL-6 levels were significantly higher in the patients than those in controls (P0.05). Conclusion: Abnormal high serum TNF-α and IL-6 levels were important pathophysiologic features in chronic bronchial asthma. (authors)

  11. Prognosis of R1-resection at the bronchial stump in patients with non-small cell lung cancer

    Institute of Scientific and Technical Information of China (English)

    Lyu Jima; Hao Xuezhi; Hui Zhouguang; Liang Jun; Zhou Zongmei; Feng Qinfu; Xiao Zefen

    2014-01-01

    Background The prognosis of R1-resection at the bronchial stump in patients with non-small cell lung cancer (NSCLC) remains unclear.This study intends to identify the prognostic factors and to optimize treatments for these patients under update conditions.Methods The data of 124 NSCLC patients who underwent R1-resection at the bronchial stump was reviewed.There were 41 patients in the surgery group (S),21 in the postoperative radiotherapy (PORT) group (S+R),30 in the postoperative chemotherapy (POCT) group (S+C),and 32 in the PORT plus POCT group (S+R+C).The constitute proportion in different groups was tested using the X2 method,univariate analysis was performed using the Kaplan-Meier and log-rank method,and multivariate analysis was done using the Cox hazard regression with entry factors including age,sex,pathological type and stage,classification of the residual disease,and treatment procedure.The process was performed stepwise backward with a maximum iteration of 20 and an entry possibility of 0.05 as well as an excluded possibility of 0.10 at each step.Results In univariate analysis,survival was more favorable for patients with squamous cell carcinoma,early pathological T or N stage,and chemotherapy or radiotherapy.There was no significant difference in the survival for patients with different types of the residual disease,except for the difference between patients with carcinoma in situ and lymphangiosis carcinomatosa (P=0.030).The survival for patients receiving chemoradiotherapy was superior to that for those undergoing surgery alone (P=0.016).In multivariate analysis,the pathological type (HR 2.51,95% CI 1.59 to 3.96,P=0.000),pathological T (HR 1.29,95% CI 1.04 to 1.60,P=-0.021) or N stage (HR 2.04,95% CI 1.40 to 2.98,P=0.000),and chemotherapy (HR 0.24,95% CI 0.13 to 0.43,P=0.000) were independent prognostic factors.Conclusion Patients with squamous cell carcinoma,early pathological T or N stage,or receiving chemotherapy had a more favorable

  12. Investigation on the quality of life of patients with bronchial asthma and its influencing factors%支气管哮喘患者生命质量及其影响因素的调查报告

    Institute of Scientific and Technical Information of China (English)

    周文君; 周鹰; 姜永前; 张学艳; 陈金磊; 顾娟

    2012-01-01

    目的 了解支气管哮喘患者的生命质量及其影响因素.方法 采用问卷调查方法 对支气管哮喘患者的生命质量及其影响因素进行横断面研究.结果 支气管哮喘患者各领域的生命质量得分与认知水平、用药依从性、哮喘控制测试表(ACT)得分呈正相关;用药依从性、教育程度是影响患者生命质量总分的保护因素,病程是影响患者生命质量总分的危险因素.结论 可采取用药指导、监督等方式对哮喘患者的用药依从性进行人为干预,从而达到提高患者生命质量的目的.%Objective To know the life quality of patients with bronchial asthma and its influencing factors.Methods By using questionnaire,the life quality of patients with bronchial asthma and its influencing factors were investigated.Results The scores of quality of life of patients with bronchial asthma were positive correlated with cognitive levels,medication compliance,the scores of asthma control test(ACT).Furthermore,the medication compliance and the education levels were the protective factors,and the diseases course was the risk factors for scores of quality of life.Conclusion To improve the quality of life of patients with bronchial asthma,medication guidance,supervision and some other measurements should be used in intervention of medication compliance.

  13. Oxidant-induced corticosteroid unresponsiveness in human bronchial epithelial cells

    NARCIS (Netherlands)

    Heijink, Irene; van Oosterhout, Antoon; Kliphuis, Nathalie; Jonker, Marnix; Hoffmann, Roland; Telenga, Eef; Klooster, Karin; Slebos, Dirk-Jan; ten Hacken, Nick; Postma, Dirkje; van den Berge, Maarten

    2014-01-01

    Background We hypothesised that increased oxidative stress, as present in the airways of asthma and chronic obstructive pulmonary disease (COPD) patients, induces epithelial damage and reduces epithelial responsiveness to suppressive effects of corticosteroids on proinflammatory cytokine production

  14. FeNO and Bronchial Responsiveness are Associated and Continuous traits in Young Children Independent of Asthma

    DEFF Research Database (Denmark)

    Schoos, Ann-Marie Malby; Chawes, Bo Lund Krogsgaard; Bønnelykke, Klaus;

    2012-01-01

    with and in children without a history of asthma symptoms. METHODS 196 six-year-old children comprising asymptomatic children, children with intermittent asthmatic symptoms and children with persistent asthma were randomly included from the Copenhagen Prospective Study on Asthma in Childhood (COPSAC2000) high......ABSTRACT BACKGROUND Elevated fraction of exhaled nitric oxide (FeNO) and bronchial hyperresponsiveness are used as surrogate markers of asthma. These traits may be continuous in the population. OBJECTIVE To investigate whether FeNO and bronchial responsiveness are associated both in children......-risk birth cohort born of mothers with asthma. Bronchial responsiveness was assessed as the relative change in specific airway resistance after cold-dry-air hyperventilation. FeNO measurements were performed prior to the hyperventilation test. The association between FeNO and bronchial responsiveness...

  15. Bronchial neuroendocrine elements in late post-radiation stage in humans after total body irradiation

    International Nuclear Information System (INIS)

    It is not known how long-term total body irradiation affects the neuroendocrine cells (Nc) and peptidergic innervation in the bronchial wall. This study examined, by immunohistochemical and radioimmunoassay (RIA) techniques, the distribution of NC and neuropeptide-containing nerve fibres in the large bronchi of Chernobyl nuclear accident cleanup workers displaying pulmonary fibrosis and metaplastic epithelium. Bronchial mucous and submucous layers from 16 Chernobyl patients and 6 control subjects were examined by conventional light microscopy and immunohistochemical techniques for determination of protein gene product 9.5 (PGP), chromogranin A, chromogranin A and B (CAB), calcitonin gene-related peptide (CGRP), calcitonin, vasoactive intestinal peptide (VIP), gastrin-releasing peptide (GRP), helospectin I, neuropeptide Y (NPY), pituitary adenylate cyclase activating peptide (PACAP), serotonin (5-hydroxyltryptamine, 5-HT), and substance P (SP). Additionally, bronchial biopsies from 6 Chernobyl cleanup workers and 3 control patients were examined by RIA for VIP and NPY/peptideYY-Ievels. The Chernobyl patients were examined 10 years after exposure during the cleanup works in the Chernobyl Atomic Electric Power Station. PGP immunoreactive nerve fibres appeared to be more frequent in the bronchial wall after long term irradiation as compared with controls. However, no specific alterations in the amounts of NPY-, PACAP-, helospectin-, SP- and CGRP-immunoreactive nerve fibres were seen in bronchi of control and Chernobyl patients. 5-HT -immunoreactive NC appeared to be more numerous in normal bronchial epithelium adjacent to metaplastic epithelium, in which numerous CAB- immunoreactive NC were seen in Chernobyl patients. RIA for VIP and NPY/PYY showed individual variations in the levels of these peptides in the bronchial tissue. In two cases (one Chernobyl patient and one control patient) there was a high concentration of VIP in parallel with a high concentration of NPY

  16. Ferritin, finger clubbing, and lung disease.

    OpenAIRE

    Shneerson, J M; Jones, B M

    1981-01-01

    The serum ferritin concentration has been determined by an immunoradiometric assay in 90 subjects with a variety of pulmonary diseases. No association between ferritin concentrations and finger clubbing has been found in any of the diseases studied. Ferritin levels were significantly raised in the subjects with bronchial carcinoma, but were not useful in monitoring recurrence of the tumour. Pulmonary artery and pulmonary vein ferritin concentrations were similar to systemic venous concentrati...

  17. Oxidative stress induced Interleukin-32 mRNA expression in human bronchial epithelial cells

    Directory of Open Access Journals (Sweden)

    Kudo Megumi

    2012-03-01

    Full Text Available Abstract Background Chronic obstructive pulmonary disease (COPD is characterized by airflow obstruction and persistent inflammation in the airways and lung parenchyma. Oxidative stress contributes to the pathogenesis of COPD. Interleukin (IL-32 expression has been reported to increase in the lung tissue of patients with COPD. Here, we show that IFNγ upregulated IL-32 expression and that oxidative stress augmented IFNγ-induced-IL-32 expression in airway epithelial cells. We further investigated transcriptional regulation responsible for IFNγ induced IL-32 expression in human airway epithelial cells. Methods Human bronchial epithelial (HBE cells were stimulated with H2O2 and IFNγ, and IL-32 expression was evaluated. The cell viability was confirmed by MTT assay. The intracellular signaling pathways regulating IL-32 expression were investigated by examining the regulatory effects of MAPK inhibitors and JAK inhibitor after treatment with H2O2 and IFNγ, and by using a ChIP assay to identify transcription factors (i.e. c-Jun, CREB binding to the IL-32 promoter. Promoter activity assays were conducted after mutations were introduced into binding sites of c-Jun and CREB in the IL-32 promoter. IL-32 expression was also examined in HBE cells in which the expression of either c-Jun or CREB was knocked out by siRNA of indicated transcription factors. Results There were no significant differences of cell viability among groups. After stimulation with H2O2 or IFNγ for 48 hours, IL-32 expression in HBE cells was increased by IFNγ and synergistically upregulated by the addition of H2O2. The H2O2 augmented IFNγ induced IL-32 mRNA expression was suppressed by a JNK inhibitor, but not by MEK inhibitor, p38 inhibitor, and JAK inhibitor I. Significant binding of c-Jun and CREB to the IL-32 promoter was observed in the IFNγ + H2O2 stimulated HBE cells. Introducing mutations into the c-Jun/CREB binding sites in the IL-32 promoter prominently suppressed its

  18. 呼吸道上皮细胞钠/氯离子通道与支气管哮喘%Epithelial sodium and chloride channels and bronchial asthma

    Institute of Scientific and Technical Information of China (English)

    王雯; 吉宏龙

    2015-01-01

    支气管哮喘(简称哮喘)是一种慢性气道疾病,表现为气道高反应性和气道炎症导致的可逆性气道阻塞.研究显示,呼吸道上皮细胞钠/氯离子通道(ENaC/CFTR)调节黏液纤毛系统从而参与了慢性气道疾病的发病机制.ENaC及CFTR共同调节黏液的水质层,从而影响气道纤毛清除能力.调节上皮通道蛋白的特异性拮抗剂或激活剂将为哮喘和其他慢性气道疾病的预防和治疗开拓新的研究前景.%Bronchial asthma (asthma) is a chronic respiratory disease characterized by reversible airway obstruction with bronchial hyper-responsiveness and inflammation.Airway cilia system is implicated in the pathogenesis of chronic airway diseases.Epithelial sodium channels (ENaC) and cystic fibrosis transmembrane conductance regulator (CFTR) are closely related to the mucociliary clearance.ENaC and CFTR jointly adjust the water layer of mucus, which affects the airway cilia clearance ability.Specific antagonists or activating agents of ENaC and CFTR could be novel pharmaceutical interventions for the prevention and treatment of asthma as well as other chronic airway diseases.

  19. Human Bronchial Epithelial Cell Response to Heavy Particle Exposure

    Science.gov (United States)

    Story, Michael; Ding, Liang-Hao; Minna, John; Park, Seong-mi; Peyton, Michael; Larsen, Jill

    2012-07-01

    A battery of non-oncogenically immortalized human bronchial epithelial cells (HBECs) are being used to examine the molecular changes that lead to lung carcinogenesis after exposure to heavy particles found in the free space environment. The goal is to ultimately identify biomarkers of radioresponse that can be used for prediction of carcinogenic risk for fatal lung cancer. Our initial studies have focused on the cell line HBEC3 KT and the isogenic variant HBEC3 KTR53, which overexpresses the RASv12 mutant and where p53 has been knocked down by shRNA, and is considered to be a more oncogenically progressed variant. We have previously described the response of HBEC3 KT at the cellular and molecular level, however, the focus here is on the rate of cellular transformation after HZE radiation exposure and the molecular changes in transformed cells. When comparing the two cell lines we find that there is a maximum rate of cellular transformation at 0.25 Gy when cells are exposed to 1 GeV Fe particles, and, for the HBEC3 KTR53 there are multiple pathways upregulated that promote anchorage independent growth including the mTOR pathway, the TGF-1 pathway, RhoA signaling and the ERK/MAPK pathway as early as 2 weeks after radiation. This does not occur in the HBEC3 KT cell line. Transformed HBEC3 KT cells do not show any morphologic or phenotypic changes when grown as cell cultures. HBEC3 KTR53 cells on the other hand show substantial changes in morphology from a cobblestone epithelial appearance to a mesenchymal appearance with a lack of contact inhibition. This epithelial to mesenchymal change in morphology is accompanied by the expression of vimentin and a reduction in the expression of E-cadherin, which are hallmarks of epithelial to mesenchymal transition. Interestingly, for HBEC3 KT transformed cells there are no mutations in the p53 gene, 2 of 15 clones were found to be heterozygous for the RASV12 mutation, and 3 of 15 clones expressed high levels of BigH3, a TGFB

  20. Trigger of bronchial hyperresponsiveness development may not always need eosinophilic airway inflammation in very early stage of asthma

    OpenAIRE

    Obase, Yasushi; Shimoda, Terufumi; Kishikawa, Reiko; Kohno, Shigeru; Iwanaga, Tomoaki

    2016-01-01

    Background: Cough variant asthma (CVA), a suggested precursor of standard bronchial asthma (SBA), is characterized by positive bronchial hyperresponsiveness (BHR) and a chronic cough response to bronchodilator that persists for >8 weeks. Objective: Airway inflammation, BHR, and airway obstructive damage were analyzed to assess whether CVA represents early or mild-stage SBA. Methods: Patients with newly diagnosed CVA (n = 72) and SBA (n = 84) naive to oral or inhaled corticosteroids and withou...

  1. Changes in bronchial responsiveness to inhaled histamine over four years in middle aged male smokers and ex-smokers.

    OpenAIRE

    Lim, T K; Taylor, R. G.; WATSON, A.; Joyce, H; Pride, N. B.

    1988-01-01

    Bronchial hyperresponsiveness to inhaled histamine in smokers is associated with an accelerated annual decline in FEV1 and low baseline FEV1 values. The evolution of bronchial hyperresponsiveness and whether it precedes or follows the accelerated decline in FEV1 and reduction in FEV1 is unknown. Measurements of the provocative concentration of inhaled histamine required to reduce FEV1 by 20% (PC20) were repeated after a four year interval in 27 male smokers (mean age 59 years, smoking on aver...

  2. Anti-IL-5 treatment reduces deposition of ECM proteins in the bronchial subepithelial basement membrane of mild atopic asthmatics

    OpenAIRE

    Flood-Page, Patrick; Menzies-Gow, Andrew; Phipps, Simon; Ying, Sun; Wangoo, Arun; Ludwig, Mara S.; Barnes, Neil; Robinson, Douglas; Kay, A. Barry

    2003-01-01

    Eosinophil-derived TGF-β has been implicated in remodeling events in asthma. We hypothesized that reduction of bronchial mucosal eosinophils with anti–IL-5 would reduce markers of airway remodeling. Bronchial biopsies were obtained before and after three infusions of a humanized, anti–IL-5 monoclonal antibody (mepolizumab) in 24 atopic asthmatics in a randomized, double-blind, placebo-controlled study. The thickness and density of tenascin, lumican, and procollagen III in the reticular baseme...

  3. Role of poly(ADP-ribose) polymerase 1 in DNA methylation changes induced by hydroquinone in human bronchial epithelial cell

    Institute of Scientific and Technical Information of China (English)

    沙炎

    2014-01-01

    Objective To investigate the DNA methylation changes induced by hydroquinone(HQ)in human bronchial epithelial cells and to explore the role of poly(ADP-ribose)polymerase-1(PARP-1)in this process.Methods Human bronchial epithelial 16HBE cells and PARP-1-deficient 16HBE cells(16HBE-shPARP-1 cells)were exposed to HQ(10,20,40,60,and 80μmol/L)for 48

  4. Efficacy of Vasa Avaleha and its granules on Tamaka Shwasa (bronchial asthma): Open-label randomized clinical study

    OpenAIRE

    Ankit M Paneliya; Patgiri, Biswajyoti; Galib, R.; Prajapati, Pradeep Kumar

    2015-01-01

    Introduction: Bronchial asthma is one of the chronic inflammatory disorders of the respiratory tract causing a huge number of deaths annually. Increased industrialization and pollution are the exacerbating factors for this situation. In Ayurveda, this miserable condition is comparable with Tamaka Shwasa. Synthetic drugs provide instant symptomatic relief in cases of bronchial asthma but are known to develop certain adverse drug reactions. Considering this, the current suffering population is ...

  5. Effect of formoterol on clinical parameters and lung functions in patients with bronchial asthma: a randomised controlled trial

    OpenAIRE

    Akpinarli, A.; Tuncer, A; Saraclar, Y.; Sekerel, B.; Kalayci, O.

    1999-01-01

    AIMS—To determine the role of formoterol in the treatment of children with bronchial asthma who are symptomatic despite regular use of inhaled corticosteroids.
METHODS—A randomised, double blind, parallel group, placebo controlled study to investigate the effects of inhaled formoterol (12 µg twice a day) in 32 children with moderate to severe bronchial asthma. The study consisted of two week run in periods and six week treatment periods, during both of which the patients ...

  6. 维生素D3与支气管哮喘%Vitamin D3 and bronchial asthma

    Institute of Scientific and Technical Information of China (English)

    丁冬胜

    2011-01-01

    支气管哮喘在世界各国都有逐年上升的趋势,大量研究显示其发生与免疫调节有密切关系,而维生素D3在支气管哮喘有其特定免疫调节机制,可通过调节细胞因子、调节性T细胞、基质金属蛋白酶-9等对支气管哮喘起到一定作用.维生素D3对支气管哮喘免疫调节可为临床有效治疗哮喘提供一定的理论依据,并最终为支气管哮喘患儿提供新的免疫治疗方法.%The morbility of bronchial asthma is gradually increasing year by year all over the world. Numerous studies have demonstrated that bronchial asthma is close related with immunoregulation. Vitamin D3 is involved to immune regulatory mechanisms in bronchial asthma, including regulating cytokine, Treg, matrix metalloproteinases-9. Considering the influence of vitamin D3 on the immune regulatory of bronchial asthma, it is provided a theory evidence that vitamin D can be used in the treatment of bronchial asthma effectivly and a novel method of immune treatment for children with bronchial asthma eventually.

  7. EXPRESSION OF SOCS3 AND SOCS5 MRNAS IN PERIPHERAL BLOOD MONONUCLEARS FROM THE PATIENTS WITH BRONCHIAL ASTHMA

    Directory of Open Access Journals (Sweden)

    V. V. Lim

    2014-01-01

    Full Text Available We observed sixty patients with allergic bronchial asthma (ABA and 54 with non-allergic bronchial asthma (NABA. Quantitative SOCS3 and SOCS5 mRNA expression was evaluated by means of real-time PCR. Eighteen healthy persons served as a control group. In patients with bronchial asthma (irrespectively of pathogenetic form, a significant increase of SOCS3 transcription factor expression was detected in peripheral blood mononuclears, as compared with control group. This increase was more pronounced in NABA group. The mRNA SOCS5 level was significantly decreased in bronchial asthma patients, as compared to control group, especially, in ABA subgroup rather than in NABA patients. Thus, an increased expression of SOCS3 mRNA in BA patients could be regarded as a protective antiinflammatory response Decrease of SOCS5 mRNA expression in patients with bronchial asthma (being more pronounced in ABA, may be indicative for a deficiency in negative feedback regulation of gene transcription in allergic bronchial asthma.

  8. Knowledge and Attitudes of GPs in Saxony-Anhalt concerning the Psychological Aspects of Bronchial Asthma: A Questionnaire Study

    Directory of Open Access Journals (Sweden)

    Werwick Katrin

    2010-12-01

    Full Text Available Abstract Bronchial Asthma is a worldwide condition with particularly high prevalence in first world countries. The reasons are multifactorial but a neglected area is the psychological domain. It is well known that heavy emotions can trigger attacks and that depression negatively affects treatment outcomes. It is also known that personality type has a greater effect on disease prevalence than in many other conditions. However, many potential psychological treatments are hardly considered, neither in treatment guidelines nor in reviews by asthma specialists. Moreover, there is very little research concerning the beliefs and practices of doctors regarding psychological treatments. Using a questionnaire survey we ascertained that local GPs in Saxony-Anhalt have reasonably good knowledge about the psychological elements of asthma; a third consider it to be some of the influence (20-40% aetiology and a further third consider it to be even more important than that (at least 40% total aetiology. Our GPs use psychosomatic counseling sometimes or usually in the areas of sport and smoking (circa 85% GPs, although less so regarding breathing techniques and relaxation (c40% usually or sometimes do this However despite this knowledge they refer to the relevant clinicians very rarely (98% sometimes, usually or always refer to a respiratory physician compared with only 11% referring for psychological help.

  9. Involvement of HIF-2α-mediated inflammation in arsenite-induced transformation of human bronchial epithelial cells

    Energy Technology Data Exchange (ETDEWEB)

    Xu, Yuan; Zhao, Yue; Xu, Wenchao; Luo, Fei; Wang, Bairu; Li, Yuan; Pang, Ying; Liu, Qizhan, E-mail: drqzliu@hotmail.com

    2013-10-15

    Arsenic is a well established human carcinogen that causes diseases of the lung. Some studies have suggested a link between inflammation and lung cancer; however, it is unknown if arsenite-induced inflammation causally contributes to arsenite-caused malignant transformation of cells. In this study, we investigated the molecular mechanisms underlying inflammation during neoplastic transformation induced in human bronchial epithelial (HBE) cells by chronic exposure to arsenite. The results showed that, on acute or chronic exposure to arsenite, HBE cells over-expressed the pro-inflammatory cytokines, interleukin-6 (IL-6), interleukin-8 (IL-8), and interleukin-1β (IL-1β). The data also indicated that HIF-2α was involved in arsenite-induced inflammation. Moreover, IL-6 and IL-8 were essential for the malignant progression of arsenite-transformed HBE cells. Thus, these experiments show that HIF-2α mediates arsenite-induced inflammation and that such inflammation is involved in arsenite-induced malignant transformation of HBE cells. The results provide a link between the inflammatory response and the acquisition of a malignant transformed phenotype by cells chronically exposed to arsenite and thus establish a previously unknown mechanism for arsenite-induced carcinogenesis. - Highlights: • Arsenite induces inflammation. • Arsenite-induced the increases of IL-6 and IL-8 via HIF-2α. • Inflammation is involved in arsenite-induced carcinogenesis.

  10. Imbalance of Peripheral Th17 and Regulatory T Cells in Children with Allergic Rhinitis and Bronchial Asthma.

    Science.gov (United States)

    Tao, Baohong; Ruan, Guiying; Wang, Dongguo; Li, Yong; Wang, Zhuping; Yin, Genquan

    2015-06-01

    The purpose of the present study is to investigate the prevalence of Th17 and regulatory T (Treg) cells in children with allergic rhinitis (AR) accompanying with bronchial asthma (BA). 24 children with AR, 22 children with BA, 18 children with AR accompanying with BA, and 20 healthy controls were recruited. The prevalence of peripheral blood Th17 and Treg cells were determined by flow cytometry. mRNA expression of retinoid-acid receptor-related orphan receptor (ROR)-γt and forkhead box P3 (Foxp3) were determined by realtime polymerase chain reaction. Cytokine expressions in plasma were determined by enzyme linked immunosorbent assay. The frequency of Th17 cells, ROR-γt mRNA expression, and the plasma levels of IL-17 were significantly higher, while Treg cells and Transforming growth factor (TGF)-β1 were significantly lower in children with AR accompanying with BA compared with those in children with AR or BA alone or control subjects. In children with allergic airway disease, total IgE levels were positively correlated to the frequency of Th17 cells (r=0.607, pTreg cells (r=-0.429, pTreg cells r=0.504, pTreg cells plays an important role in the pathogenesis of AR accompanying with BA. PMID:26546895

  11. TRANSCRIPTIONAL FA CTORS FOXP3, GATA-3, PAX-5 AND THEIR COOPERAT IVE INTERACTIONS IN BRONCHIAL ASTHMA

    Directory of Open Access Journals (Sweden)

    V. N. Mineev

    2013-01-01

    Full Text Available Abstract. The review article summarizes current information about transcriptional factors Fохр3, GATA- 3, РАХ-5 and their cooperative interactions. Predomination of one of these factors leads to production of corresponding cytokines and appropriate changes in T and B cell functions, thus causing development of different inflammatory events and typical symptoms. Focusing the discussion on Foxp3, GATA-3 and PAX- 5 is essential for understanding pathogenesis of inflammatory lung diseases, in particular, bronchial asthma. The data obtained will be helpful in development of novel therapeutic strategies. Mechanisms of cooperative interactions of Fохр3, GATA-3 and РАХ-5, generally, remain poorly understood. In this article, we present modern, sometimes, controversial views on this issue. In particular, we are discussing regulation of Foxp3 expression, molecular interactions affecting production of PAX-5 factor, as well as addressing possible indirect interactions between these transcription factors via implementing GATA-3 and STAT6 functions.

  12. Lung function and bronchial responsiveness after Mycoplasma pneumoniae infection in early childhood

    DEFF Research Database (Denmark)

    Boysen, Birgitte Kjær; Jensen, Jørgen S; Nielsen, Kim G;

    2008-01-01

    follow-up examination including lung function testing (28 PCR-positive and 37 PCR-negative). In addition to the PCR-test for M. pneumoniae all respiratory tract specimens were additionally tested for other atypical bacteria and for viruses by PCR. Lung function was measured as specific airway resistance...... on lung function and bronchial responsiveness. In a retrospective, clinical cohort-study children younger than 5 years-of-age when PCR-tested for M. pneumoniae were enrolled. Sixty-five children with clinical symptoms suggesting infection with M. pneumoniae during an epidemic season completed a clinical...... 1.21 (kPa sec), P = 0.45; and mean change in specific resistance was 13% versus 9%, P = 0.42. In conclusion, M. pneumoniae infection in early childhood was not associated with long-term effects on lung function and bronchial hyperresponsiveness 2 years after infection....

  13. "Bronchial Artery Delivery of Viral Vectors for Gene delivery in Cystic Fibrosis; Superior to Airway Delivery?"

    Directory of Open Access Journals (Sweden)

    Coutelle Charles C

    2002-04-01

    Full Text Available Abstract Background Attempts at gene therapy for the pulmonary manifestations of Cystic Fibrosis have relied mainly on airway delivery. However the efficiency of gene transfer and expression in the airway epithelia has not reached therapeutic levels. Access to epithelial cells is not homogenous for a number of reasons and the submucosal glands cannot be reached via the airways. Presentation We propose to inject gene delivery vectors directly into bronchial arteries combined with pre-delivery of vascular endothelial growth factor to increase vascular endothelial permeability and post-delivery flow reduction by balloon occlusion. Thus it may be possible to reach mucous secreting cells of the bronchial luminal epithelium and the submucosal glands in an increased and homogenous fashion. Testing This combination of techniques to the best of our knowledge has not previously been investigated, and may enable us to overcome some of the current limitations to gene therapy for Cystic Fibrosis.

  14. Effect of parenteral magnesium sulfate on pulmonary functions in bronchial asthma.

    Science.gov (United States)

    Sharma, S K; Bhargava, A; Pande, J N

    1994-01-01

    Eighteen patients with bronchial asthma were studied in a single-blind fashion. Each patient received an infusion of magnesium sulfate (MgSO4.7H2O 50% W/V) in a dose equivalent to 10 mmol of elemental magnesium. The airways resistance showed a significant decrease (p significantly (p V50 (p statistically significant increase (p Significant changes in Raw (p significant side effects were observed with MgSO4 infusion. It is concluded that modest improvement in airways resistance and specific conductance and only small improvement in maximal expiratory flow rates is consistent with a predominant effect of MgSO4 on large airways in patients with bronchial asthma.

  15. Bronchial effects of leukotriene D4 inhalation in normal human lung

    DEFF Research Database (Denmark)

    Bisgaard, H; Groth, S

    1987-01-01

    The aim of the study was to investigate whether inhaled leukotriene (LT) D4 could mimic the characteristics of asthmatic patients after allergen-induced attack, i.e. a prolonged subclinical bronchial obstruction, an increased reactivity of the airways and a late reaction. The effects of LTD4 were...... airways in asthmatic patients out of attack. LTD4 caused a dose-dependent obstruction of the airways as measured by partial flow-volume curves and volume of trapped gas, yet only minor changes in forced expiratory volume in 1 s (FEV1) and peak expiratory flow rate. LTD4 was 1900-7000 times more potent...... than histamine. LTD4 inhalations were almost symptomless as opposed to the irritative and dyspnoeic symptoms seen after inhalation of histamine. The time duration for the induced change in partial flow-volume curves was the same for the two drugs. Approximately 30 min elapsed until the bronchial...

  16. Blood lipid levels associate with childhood asthma, airway obstruction, bronchial hyperresponsiveness, and aeroallergen sensitization

    DEFF Research Database (Denmark)

    Vinding, Rebecca K; Stokholm, Jakob; Chawes, Bo Lund Krogsgaard;

    2016-01-01

    with assessments of lung function, bronchial responsiveness, fraction of exhaled nitric oxide (Feno), and allergic sensitization. Associations between lipid levels and clinical outcomes were adjusted for sex, passive smoking, and body mass index. RESULTS: High levels of low-density lipoprotein cholesterol were......BACKGROUND: Studies of children's blood lipid profiles in relation to asthma are few, and the results are ambiguous. OBJECTIVE: We sought to examine whether the lipid profile is associated with concurrent asthma, altered lung function, and allergic sensitization in children. METHODS: High...... of increased Feno levels (aβ coefficient, 0.14 log-ppb; 95% CI, -0.02 to 0.30 log-ppb; P = .08). CONCLUSION: The blood lipid profile is associated with asthma, airway obstruction, bronchial responsiveness, and aeroallergen sensitization in 7-year-old children. These findings suggest that asthma and allergy...

  17. Respiratory syncytial virus inhibits ciliagenesis in differentiated normal human bronchial epithelial cells: effectiveness of N-acetylcysteine.

    Science.gov (United States)

    Mata, Manuel; Sarrion, Irene; Armengot, Miguel; Carda, Carmen; Martinez, Isidoro; Melero, Jose A; Cortijo, Julio

    2012-01-01

    Persistent respiratory syncytial virus (RSV) infections have been associated with the exacerbation of chronic inflammatory diseases, including chronic obstructive pulmonary disease (COPD). This virus infects the respiratory epithelium, leading to chronic inflammation, and induces the release of mucins and the loss of cilia activity, two factors that determine mucus clearance and the increase in sputum volume. These alterations involve reactive oxygen species-dependent mechanisms. The antioxidant N-acetylcysteine (NAC) has proven useful in the management of COPD, reducing symptoms, exacerbations, and accelerated lung function decline. NAC inhibits RSV infection and mucin release in human A549 cells. The main objective of this study was to analyze the effects of NAC in modulating ciliary activity, ciliagenesis, and metaplasia in primary normal human bronchial epithelial cell (NHBEC) cultures infected with RSV. Our results indicated that RSV induced ultrastructural abnormalities in axonemal basal bodies and decreased the expression of β-tubulin as well as two genes involved in ciliagenesis, FOXJ1 and DNAI2. These alterations led to a decrease in ciliary activity. Furthermore, RSV induced metaplastic changes to the epithelium and increased the number of goblet cells and the expression of MUC5AC and GOB5. NAC restored the normal functions of the epithelium, inhibiting ICAM1 expression, subsequent RSV infection through mechanisms involving nuclear receptor factor 2, and the expression of heme oxygenase 1, which correlated with the restoration of the antioxidant capacity, the intracellular H(2)O(2) levels and glutathione content of NHBECs. The results presented in this study support the therapeutic use of NAC for the management of chronic respiratory diseases, including COPD.

  18. Respiratory syncytial virus inhibits ciliagenesis in differentiated normal human bronchial epithelial cells: effectiveness of N-acetylcysteine.

    Directory of Open Access Journals (Sweden)

    Manuel Mata

    Full Text Available Persistent respiratory syncytial virus (RSV infections have been associated with the exacerbation of chronic inflammatory diseases, including chronic obstructive pulmonary disease (COPD. This virus infects the respiratory epithelium, leading to chronic inflammation, and induces the release of mucins and the loss of cilia activity, two factors that determine mucus clearance and the increase in sputum volume. These alterations involve reactive oxygen species-dependent mechanisms. The antioxidant N-acetylcysteine (NAC has proven useful in the management of COPD, reducing symptoms, exacerbations, and accelerated lung function decline. NAC inhibits RSV infection and mucin release in human A549 cells. The main objective of this study was to analyze the effects of NAC in modulating ciliary activity, ciliagenesis, and metaplasia in primary normal human bronchial epithelial cell (NHBEC cultures infected with RSV. Our results indicated that RSV induced ultrastructural abnormalities in axonemal basal bodies and decreased the expression of β-tubulin as well as two genes involved in ciliagenesis, FOXJ1 and DNAI2. These alterations led to a decrease in ciliary activity. Furthermore, RSV induced metaplastic changes to the epithelium and increased the number of goblet cells and the expression of MUC5AC and GOB5. NAC restored the normal functions of the epithelium, inhibiting ICAM1 expression, subsequent RSV infection through mechanisms involving nuclear receptor factor 2, and the expression of heme oxygenase 1, which correlated with the restoration of the antioxidant capacity, the intracellular H(2O(2 levels and glutathione content of NHBECs. The results presented in this study support the therapeutic use of NAC for the management of chronic respiratory diseases, including COPD.

  19. Respiratory syncytial virus inhibits ciliagenesis in differentiated normal human bronchial epithelial cells: effectiveness of N-acetylcysteine.

    Science.gov (United States)

    Mata, Manuel; Sarrion, Irene; Armengot, Miguel; Carda, Carmen; Martinez, Isidoro; Melero, Jose A; Cortijo, Julio

    2012-01-01

    Persistent respiratory syncytial virus (RSV) infections have been associated with the exacerbation of chronic inflammatory diseases, including chronic obstructive pulmonary disease (COPD). This virus infects the respiratory epithelium, leading to chronic inflammation, and induces the release of mucins and the loss of cilia activity, two factors that determine mucus clearance and the increase in sputum volume. These alterations involve reactive oxygen species-dependent mechanisms. The antioxidant N-acetylcysteine (NAC) has proven useful in the management of COPD, reducing symptoms, exacerbations, and accelerated lung function decline. NAC inhibits RSV infection and mucin release in human A549 cells. The main objective of this study was to analyze the effects of NAC in modulating ciliary activity, ciliagenesis, and metaplasia in primary normal human bronchial epithelial cell (NHBEC) cultures infected with RSV. Our results indicated that RSV induced ultrastructural abnormalities in axonemal basal bodies and decreased the expression of β-tubulin as well as two genes involved in ciliagenesis, FOXJ1 and DNAI2. These alterations led to a decrease in ciliary activity. Furthermore, RSV induced metaplastic changes to the epithelium and increased the number of goblet cells and the expression of MUC5AC and GOB5. NAC restored the normal functions of the epithelium, inhibiting ICAM1 expression, subsequent RSV infection through mechanisms involving nuclear receptor factor 2, and the expression of heme oxygenase 1, which correlated with the restoration of the antioxidant capacity, the intracellular H(2)O(2) levels and glutathione content of NHBECs. The results presented in this study support the therapeutic use of NAC for the management of chronic respiratory diseases, including COPD. PMID:23118923

  20. 150例小儿过敏性支气管哮喘发病因素分析%Analysis of 150 cases of Chidren allergic bronchial asthma

    Institute of Scientific and Technical Information of China (English)

    杨丽萍

    2016-01-01

    Objective To investigate the relative factors of the incidence of allergic bronchial asthma in children with 150 cases of allergic bronchial asthma. Methods A total of 150 cases of allergic bronchial asthma children as the object of study in the observation group, February 2015 to 2016 February by the hospital treatment of children, in addition to select February 2015 to 2016 February by the hospital treatment of 150 cases of non allergic bronchial asthma children as the research object in the control group, by the self-made questionnaire on the incidence of the two groups of children related infection factors, immune factors, environmental factors and other factors were analyzed. Results an observation group and a control group of children with recurrent respiratory tract infection history, food allergy, drug allergy, paint, a history of exposure to fumes, 2 years old in passive smoking history, family history of asthma, history of pet keeping, breastfeeding history data and control group compared the difference, with significant. Conclusion There are many factors of allergic bronchial asthma should be early prevention, early detection and early treatment, to reduce the incidence of the disease and promote the health of children and growth. The research in clinical has significant value.%目的:通过对150例过敏性支气管哮喘的患儿进行调查研究,探究与小儿过敏性支气管哮喘发病的相关因素。方法选取150例过敏性支气管哮喘患儿作为本次的研究对象中的观察组,均为2015年2月-2016年2月经由该院治疗的患儿,另外选取2015年2月-2016年2月经由该院治疗的150例非过敏性支气管哮喘患儿作为该次研究对象中的对照组,通过该院自制的调查问卷对两组患儿发病有关的感染因素、免疫因素、环境因素与其他因素进行分析。结果观察组与对照组患儿的反复呼吸道感染史、食物过敏、药物过敏、油漆、油烟接触史,2岁内被动

  1. Effects of sex hormones on bronchial reactivity during the menstrual cycle

    OpenAIRE

    Matteis, Maria; Polverino, Francesca; Spaziano, Giuseppe; Roviezzo, Fiorentina; Santoriello, Carlo; Sullo, Nikol; Bucci, Maria Rosaria; Rossi, Francesco; Polverino, Mario; Owen, Caroline A.; D’Agostino, Bruno

    2014-01-01

    Background: Many asthmatic women complain of symptom exacerbations in particular periods, i.e. during pregnancy and menstrual cycles (perimenstrual asthma: PMA)". The goal of this study was to study the effect of the luteal and follicular phases of the menstrual cycle on bronchial reactivity (BR) in a group of asthmatic women. Methods: For this purpose, 36 pre-menopausal women were enrolled and underwent testing for resting pulmonary function, measurement of the diffusing capacity of the lung...

  2. Effects of sex hormones on bronchial reactivity during the menstrual cycle

    OpenAIRE

    Matteis, Maria; Polverino, Francesca; Spaziano, Giuseppe; Roviezzo, Fiorentina; Santoriello, Carlo; Sullo, Nikol; Bucci, Maria Rosaria; Rossi, Francesco; Polverino, Mario; Owen, Caroline A.; D’Agostino, Bruno

    2014-01-01

    Background Many asthmatic women complain of symptom exacerbations in particular periods, i.e. during pregnancy and menstrual cycles (perimenstrual asthma: PMA)". The goal of this study was to study the effect of the luteal and follicular phases of the menstrual cycle on bronchial reactivity (BR) in a group of asthmatic women. Methods For this purpose, 36 pre-menopausal women were enrolled and underwent testing for resting pulmonary function, measurement of the diffusing capacity of the lung f...

  3. GATA-3 EXPRESSION IN PERIPHERAL BLOOD LYMPHOCYTES OF PATIENTS WITH BRONCHIAL ASTHMA

    OpenAIRE

    Mineev, V.N.; L. N. Sorokina; M. A. Nyoma; V. I. Trofimov

    2014-01-01

    The aim of the study is to establish the features of expression of GATA-3 in peripheral lymphocytes from bronchial asthma patients (BA). Material and methods. 10 healthy controls, 15 patients with allergic (atopic) and 15 persons with non-allergic BA were examined. A transcription factor GATA-3 expressed in peripheral lymphocytes was analyzed by Western blot after the lymphocytes were lysed. Preparation of cell lysates, and Western blotting were performed by means of a standard procedure (Ame...

  4. Quality of life and activity of children suffering from bronchial asthma

    OpenAIRE

    Trzcieniecka-Green A; Bargiel-Matusiewicz K; Wilczynska-Kwiatek A

    2009-01-01

    Abstract Objective The aim of this article was to analyze the quality of life of children suffering from bronchial asthma and to analyze their everyday activity. Methods The research was conducted in 137 children. The following questionnaires were used in the study: Pediatric Asthma Quality of Life Questionnaire (PAQLQ) and a questionnaire designed by the authors specifically for the purpose of the study. Results The findings of the study show that illness duration (r = -0.2; P = 0.22) and ge...

  5. Bronchial stenosis after tuberculosis - a case report; Estenose bronquica apos tuberculose - relato de um caso

    Energy Technology Data Exchange (ETDEWEB)

    Martins, Renata Romano; Nogueira, Aline Silva; Almeida, Fabiola Assuncao de; Santos, Teresa Cristina C.R.S. dos; Marchiori, Edson; Sales, Anderson Ribeiro [Universidade Federal Fluminense, Niteroi, RJ (Brazil). Dept. de Radiologia; Lima, Carlos Eduardo T. [Universidade Federal Fluminense, Niteroi, RJ (Brazil). Servico de Cirurgia Toracica

    1999-09-01

    The authors report a case of bronchial stenosis after pulmonary tuberculosis infection in a 46 year-old man presenting symptoms related to upper left lobe collapse, that was identified by chest roentgenograms. The evaluation by computed tomography showed narrowing of the left main bronchus and obliteration of the upper left bronchus. The patient had a postero-lateral thoracotomy, lower left bronchus and left main bronchus bronchoplasty, with upper left lobectomy. (author)

  6. Carbon black and titanium dioxide nanoparticles elicit distinct apoptotic pathways in bronchial epithelial cells

    OpenAIRE

    Baeza-Squiban Armelle; Fleury Jocelyne; Martens Johan A; Andreau Karine; Borot Marie-Caroline; Ferecatu Ioana; Thomassen Leen CJ; Hussain Salik; Marano Francelyne; Boland Sonja

    2010-01-01

    Abstract Background Increasing environmental and occupational exposures to nanoparticles (NPs) warrant deeper insight into the toxicological mechanisms induced by these materials. The present study was designed to characterize the cell death induced by carbon black (CB) and titanium dioxide (TiO2) NPs in bronchial epithelial cells (16HBE14o- cell line and primary cells) and to investigate the implicated molecular pathways. Results Detailed time course studies revealed that both CB (13 nm) and...

  7. Bronchial hyperresponsiveness in an adult population in Helsinki: decreased FEV1, the main determinant

    OpenAIRE

    Juusela, Maria; Pallasaho, Paula; Sarna, Seppo; Piirilä, Päivi; Lundbäck, Bo; Sovijärvi, Anssi

    2013-01-01

    Introduction Bronchial hyperresponsiveness (BHR) elevates the risk for development of respiratory symptoms and accelerates the decline in forced expiratory volume in the first second (FEV1). We thus aimed to assess the prevalence, determinants and quantity of BHR in Helsinki. Objectives This study involved 292 randomly selected subjects age 26–66 years, women comprising 58%. Methods Following a structured interview, a spirometry, a bronchodilation test, and a skin-prick test, we assessed a br...

  8. Bronchial thermoplasty: a new therapeutic option for the treatment of severe, uncontrolled asthma in adults.

    OpenAIRE

    Marie-Christine Dombret; Khuder Alagha; Louis Philippe Boulet; Pierre Yves Brillet; Guy Joos; Michel Laviolette; Renaud Louis; Thierry Rochat; Paola Soccal; Michel Aubier; Pascal Chanez

    2014-01-01

    Bronchial thermoplasty is a young yet promising treatment for severe asthma whose benefit for long-term asthma control outweighs the short-term risk of deterioration and hospitalisation in the days following the treatment. It is an innovative treatment whose clinical efficacy and safety are beginning to be better understood. Since this is a device-based therapy, the overall evaluation of risk-benefit is unlike that of pharmaceutical products; safety aspects, regulatory requirements, study des...

  9. Long-term (5 year) safety of bronchial thermoplasty: Asthma Intervention Research (AIR) trial.

    OpenAIRE

    Thomson, NC; Rubin, AS; Niven, RM; Corris, PA; Siersted, HC; Olivenstein, R.; Pavord, ID; McCormack, D.; Laviolette, M.; Shargill, NS; Cox, G.

    2011-01-01

    BACKGROUND: Bronchial thermoplasty (BT) is a bronchoscopic procedure that improves asthma control by reducing excess airway smooth muscle. Treated patients have been followed out to 5 years to evaluate long-term safety of this procedure. METHODS: Patients enrolled in the Asthma Intervention Research Trial were on inhaled corticosteroids ≥200 μg beclomethasone or equivalent + long-acting-beta2-agonists and demonstrated worsening of asthma on long-acting-β2-agonist withdrawal. Following initial...

  10. Long term (5 Year) safety of bronchial thermoplasty: Asthma Intervention Research (AIR) trial

    OpenAIRE

    Thomson, N C; Rubin, A.S.; Niven, R. M.; Corris, P A; Siersted, H. C.; Olivenstein, R.; Pavord, I.D.; McCormick, D.; Laviolette, M.; Shargill, N.S.; Cox, G.

    2011-01-01

    Background: Bronchial thermoplasty (BT) is a bronchoscopic procedure that improves asthma control by reducing excess airway smooth muscle. Treated patients have been followed out to 5 years to evaluate long-term safety of this procedure. Methods: Patients enrolled in the Asthma Intervention Research Trial were on inhaled corticosteroids ≥200 μg beclomethasone or equivalent + long-acting-beta2-agonists and demonstrated worsening of asthma on long-acting-β2-agonist withdrawal. ...

  11. Bronchial Artery Embolization in the Management of Pulmonary Parenchymal Endometriosis with Hemoptysis

    International Nuclear Information System (INIS)

    Pulmonary parenchymal endometriosis is extremely rare and usually manifests itself with a recurrent hemoptysis associated with the menstrual cycle. The therapies proposed for women with endometriosis consist of medical treatments and surgery. Bronchial artery embolization has become a well-established and minimally invasive treatment modality for hemoptysis, and to the best of our knowledge, it has not been reported in pulmonary endometriosis. We report a case of pulmonary parenchymal endometriosis treated with embolotheraphy for hemoptysis.

  12. 320 Development of a Questionnaire for the Assessment of Bronchial Hyperresponsiveness in Korea

    OpenAIRE

    Lim, Seong Yong; Yoo, Kwang Ha; Shim, Jae-Jeong; Kim, Kwan Hyung

    2012-01-01

    Background Bronchial hyperresponsiveness (BHR) is an important pathophysiological feature of asthma. In addition to the diagnostic significance, BHR is associated with the severity of airway inflammation and BHR- based treatment approaches has been shown to be effective. Nevertheless, challenge tests are time consuming, inconvenient to patients, and are not accessible in every primary care physicians. We aimed to develop a questionnaire for the assessment of BHR in Korean subjects. Methods Fr...

  13. Action mechanisms of complex spa therapy on bronchial asthma. 1. Relationship to evaluation of spa therapy

    OpenAIRE

    Mitsunobu, Fumihiro; Mifune, Takashi; Hosaki, Yasuhiro; Ashida, Kozo; Yokota, Satoshi; Tsugeno, Hirofumi; Takeuchi, Kazuaki; Nawa, Yuichiro; Tanizaki,Yoshiro; Ochi, Koji; Harada, Hideo

    1996-01-01

    Action mechanisms of complex spa therapy (swimming training in a hot spring pool, inhalation with iodine salt solution, and fango therapy) were studied in relation to patient age, clinical asthma type, and airway inflammation. Actions of spa therapy were observed by two clinical effects : direct and indirect effects. Improvement of subjective symptoms, ventilatory function, and bronchial hyperresponsiveness was observed as direct effects of spa therapy, and improvement of suppressed function ...

  14. Effects of swimming training on aerobic capacity and exercise induced bronchoconstriction in children with bronchial asthma

    OpenAIRE

    Matsumoto, I.; Araki, H; Tsuda, K; Odajima, H; Nishima, S; Higaki, Y.; Tanaka, H.; Tanaka, M.; Shindo, M

    1999-01-01

    BACKGROUND—A study was undertaken to determine whether swimming training improved aerobic capacity, exercise induced bronchoconstriction (EIB), and bronchial responsiveness to inhaled histamine in children with asthma.
METHODS—Eight children with mild or moderate asthma participated in swimming training every day for six weeks. The intensity of training was individually determined and set at 125% of the child's lactate threshold (LT), measured using a swimming ergometer. ...

  15. Role of lung scanning in assessing the resectability of bronchial carcinoma.

    OpenAIRE

    Ellis, D A; Hawkins, T.; Gibson, G. J.; Nariman, S

    1983-01-01

    Ventilation and perfusion lung scans were performed in 58 patients before operation for bronchial carcinoma to determine in which patients the lung scan was most useful for assessing mediastinal spread and resectability of the tumour. Perfusion of the affected lung was less with larger and more centrally situated tumours. Perfusion was also less for left-sided than for right-sided tumours but this is explained by the normal differential perfusion of the right and left lungs. The lung scan was...

  16. The Impact of Allergic Rhinitis and Asthma on Human Nasal and Bronchial Epithelial Gene Expression

    OpenAIRE

    Ariane H. Wagener; Aeilko H Zwinderman; Luiten, Silvia; Fokkens, Wytske J; Bel, Elisabeth H; Sterk, Peter J.; van Drunen, Cornelis M

    2013-01-01

    Background The link between upper and lower airways in patients with both asthma and allergic rhinitis is still poorly understood. As the biological complexity of these disorders can be captured by gene expression profiling we hypothesized that the clinical expression of rhinitis and/or asthma is related to differential gene expression between upper and lower airways epithelium. Objective Defining gene expression profiles of primary nasal and bronchial epithelial cells from the same individua...

  17. Rhinovirus-induced modulation of gene expression in bronchial epithelial cells from subjects with asthma

    OpenAIRE

    Bochkov, YA; Hanson, KM; Keles, S.; Brockman-Schneider, RA; Jarjour, NN; Gern, JE

    2009-01-01

    Rhinovirus (RV) infections trigger asthma exacerbations. Genome-wide expression analysis of RV1A-infected primary bronchial epithelial cells from normal and asthmatic donors was performed to determine whether asthma is associated with a unique pattern of RV-induced gene expression. Virus replication rates were similar in cells from normal and asthmatic donors. Overall, RV downregulated 975 and upregulated 69 genes. Comparisons of transcriptional profiles generated from microarrays and confirm...

  18. Testing bronchial hyper-responsiveness: provocation or peak expiratory flow variability?

    OpenAIRE

    Otter, J.J. den; Reijnen, G.M.W.; Bosch, W.J.H.M. van den; van Schayck, C. P.; Molema, J.; van Weel, C

    1997-01-01

    BACKGROUND: Assessing bronchial hyper-responsiveness (BHR) is a main diagnostic criterion of asthma. Provocation testing is not readily available in general practice, but peak expiratory flow (PEF) is. Several guidelines promote the use of PEF variability as a diagnostic tool for BHR. This study tested the agreement between histamine challenge testing and PEF variability, and the consequences for diagnosing asthma. AIM: To investigate the possibility of assessing BHR by PEF variability, using...

  19. Asymptomatic bronchial hyperreactivity and the development of asthma and other respiratory tract illnesses in children.

    OpenAIRE

    Jones, A.

    1994-01-01

    BACKGROUND--It is not clear whether asymptomatic bronchial hyperresponsiveness (BHR) in children is a risk factor for the subsequent development of asthma. A longitudinal study was conducted to determine the predictive value of BHR for the development of asthma in a primary care patient population. METHODS--A standard free running asthma screening test (FRAST) was applied to 956 schoolchildren aged between 4 and 11 years in 1985. Peak expiratory flow (PEF) rates were measured before hard runn...

  20. Fibroblast-myofibroblast transition is differentially regulated by bronchial epithelial cells from asthmatic children

    OpenAIRE

    Reeves, Stephen R; Kolstad, Tessa; Lien, Tin-Yu; Herrington-Shaner, Sarah; Debley, Jason S.

    2015-01-01

    Background Airway remodeling is a proposed mechanism that underlies the persistent loss of lung function associated with childhood asthma. Previous studies have demonstrated that human lung fibroblasts (HLFs) co-cultured with primary human bronchial epithelial cells (BECs) from asthmatic children exhibit greater expression of extracellular matrix (ECM) components compared to co-culture with BECs derived from healthy children. Myofibroblasts represent a population of differentiated fibroblasts...

  1. Epidemiology of bronchial asthma and asthma control assessment in Henan Province, China

    OpenAIRE

    Zhang, Wenping; Chen, Xianliang; Ma, Lijun; Wu, Jizhen; Zhao, Limin; Kuang, Hongyan; Huang, Taibo; Cheng, Jianjian; Zhang, Luoxian; Qi, Yong; Sun, Beibei; Niu, Hongyan

    2014-01-01

    Background Prevalence of bronchial asthma, asthma treatment assessment, and estimation of the control level among asthma patients in Henan Province, China are reported in this paper. Methods We selected 10 among the 109 cities and districts in Henan province using a multistage stratified cluster random sampling method. A total of 500 households from each city and district were chosen. Approximately 20,000 residents from a total of 5,000 households were randomly selected to answer a questionna...

  2. Hemoptysis: a rare cause can be related to a bronchial varix due to pulmonary venous obstruction

    Energy Technology Data Exchange (ETDEWEB)

    Wiebe, Sheldon; Maclusky, Ian; Manson, David; Holowka, Stephanie; Yoo, Shi-Joon [The Hospital for Sick Children, 555 University Avenue, M5G 1X8, Toronto, Ontario (Canada)

    2003-12-01

    Bronchial varices, which have rarely been described in the radiology literature, can be the result of pulmonary venous obstruction and may present with hemoptysis. This case is an illustration of this rare condition, which correlates CT findings with bronchoscopic findings. We also describe the findings on phase-contrast MR that demonstrated reversed diastolic flow in the branch pulmonary artery supplying the affected lung. (orig.)

  3. FDG uptake at the bronchial stump after curative lobectomy for non-small cell lung cancer

    Energy Technology Data Exchange (ETDEWEB)

    Keyzer, Caroline; Corbusier, Florence; Kyratzi, Eirini; Gevenois, Pierre Alain [Universite libre de Bruxelles, Department of Radiology, Hopital Erasme, Brussels (Belgium); Sokolow, Youri [Universite libre de Bruxelles, Department of Thoracic Surgery, Hopital Erasme, Brussels (Belgium); Goldman, Serge [Universite libre de Bruxelles, Department of Nuclear Medicine, Hopital Erasme, Brussels (Belgium)

    2016-05-15

    Focal areas of FDG uptake may occur at the bronchial stump following curative lobectomy for non-small-cell lung carcinoma (NSCLC), even in the absence of suspicious CT changes. The purpose of our study was to investigate the significance of such PET/CT findings. FDG-PET/CT scans performed in 54 patients after lobectomy for NSCLC were reviewed. The presence of focal areas of FDG uptake at the bronchial stump, associated CT abnormalities, SUVmax, and normalized SUV (SUVnorm = SUVmax/mean liver SUV) were recorded. Final diagnosis was based on biopsy or imaging follow-up. Focal areas of FDG uptake at the bronchial stump were detected in 30 patients (56 %). Mean SUVmax was 4.0 ± 1.9 (range; 2.2-12.1) and mean SUVnorm was 1.8 ± 0.8 (range; 0.9-4.5). Biopsy showed recurrence in two patients. In these patients, SUVnorm was respectively 4.4 and 4.5 (with SUVmax of 8.8 and 12.1), whereas SUVnorm was lower than 4.0 in those without recurrence, with mean SUVnorm of 1.6 ± 0.5 (range; 0.9-3.4) and mean SUVmax of 3.6 ± 0.9 (range; 2.2-5.8). The CT component of the PET/CT revealed ill-defined peribronchial soft tissue opacity only in both patients with recurrence. FDG uptake at the bronchial stump is a frequent finding after pulmonary lobectomy. Moderate levels of FDG uptake (i.e., SUVnorm < 4.0) without corresponding abnormal CT findings might be a dual criterion for diagnosing benign post-surgical changes. (orig.)

  4. Objective quantitative analysis of eosinophils and bronchial epithelial cells in induced sputum by laser scanning cytometry

    OpenAIRE

    Woltmann, G; Ward, R.; Symon, F; Rew, D.; Pavord, I.; Wardlaw, A

    1999-01-01

    BACKGROUND—Sputum induction is an important non-invasive technique for measuring airway inflammation in asthma. Cell numbers are often too low for flow cytometric analysis. Laser scanning cytometry (LSC) is a novel technique that allows objective multicolour fluorescence analysis of cells on a microscope slide.
METHODS—LSC was used to determine sputum eosinophil and bronchial epithelial cell counts. We first confirmed that we could measure eosinophil counts accurately in ...

  5. A PATHOGENETIC ROLE OF TRANSCRIPTION COREPRESSOR Id2 IN BRONCHIAL ASTHMA

    Directory of Open Access Journals (Sweden)

    V. N. Mineev

    2016-01-01

    Full Text Available The aim of present study was to evaluate a possible role of Id2 (inhibitor of DNA-binding 2 in pathogenesis of bronchial asthma (BA. We have examined six healthy control persons, nine patients with allergic bronchial asthma (ABA and twenty-four patients with non-allergic bronchial asthma (NABA. The Id2 mRNA expression was evaluated by means of real-time PCR.No significant differences in Id2 mRNA expression levels were revealed between the control and asthma groups. Correlation analysis of Id2 mRNA expression has revealed significant positive correlation with AID (activation-induced cytidine deaminase mRNA levels and peripheric blood eosinophil contents in ABA. Healthy persons demonstrate strong positive correlation between Id2 mRNA expression and serum IgE levels, like as with ε-chain mRNA contents.It was suggested that the Id2 dysfunction may take place in the BA patients. These changes affect negative control over the AID expression, which is important for switching B-cells to IgE production.

  6. Possible Role of DNA Polymerase beta in Protecting Human Bronchial Epithelial Cells Against Cytotoxicity of Hydroquinone

    Institute of Scientific and Technical Information of China (English)

    DA-LIN HU; JIAN-PING YANG; DAO-KUI FANG; YAN SHA; XIAO-ZHI TU; ZHI-XIONG ZHUANG; HUAN-WEN TANG; HAI-RONG LIANG; DONG-SHENG TANG; YI-MING LIU; WEI-DONG JI; JIAN-HUI YUAN; YUN HE; ZHENG-YU ZHU

    2007-01-01

    Objective To explore the toxicological mechanism of hydroquinone in human bronchial epithelial cells and to investigate whether DNA polymerase beta is involved in protecting cells from damage caused by hydroquinone. Methods DNA polymerase beta knock-down cell line was established via RNA interference as an experimental group. Normal human bronchial epithelial cells and cells transfected with the empty vector of pEGFP-Cl were used as controls. Cells were treated with different concentrations of hydroquinone (ranged from 10 μmol/L to 120 μmol/L) for 4 hours. MTT assay and Comet assay [single-cell gel electrophoresis (SCGE)] were performed respectively to detect the toxicity of hydroquinone. Results MTT assay showed that DNA polymerase beta knock-down cells treated with different concentrations of hydroquinone had a lower absorbance value at 490 nm than the control cells in a dose-dependant manner. Comet assay revealed that different concentrations of hydroquinone caused more severe DNA damage in DNA polymerase beta knock-down cell line than in control cells and there was no significant difference in the two control groups. Conclusions Hydroquinone has significant toxicity to human bronchial epithelial cells and causes DNA damage. DNA polymerase beta knock-down cell line appears more sensitive to hydroquinone than the control cells. The results suggest that DNA polymerase beta is involved in protecting cells from damage caused by hydroquinone.

  7. CT-based geometry analysis and finite element models of the human and ovine bronchial tree.

    Science.gov (United States)

    Tawhai, Merryn H; Hunter, Peter; Tschirren, Juerg; Reinhardt, Joseph; McLennan, Geoffrey; Hoffman, Eric A

    2004-12-01

    The interpretation of experimental results from functional medical imaging is complicated by intersubject and interspecies differences in airway geometry. The application of computational models in understanding the significance of these differences requires methods for generation of subject-specific geometric models of the bronchial airway tree. In the current study, curvilinear airway centerline and diameter models have been fitted to human and ovine bronchial trees using detailed data segmented from multidetector row X-ray-computed tomography scans. The trees have been extended to model the entire conducting airway system by using a volume-filling algorithm to generate airway centerline locations within detailed volume descriptions of the lungs or lobes. Analysis of the geometry of the scan-based and model-based airways has verified their consistency with measures from previous anatomic studies and has provided new anatomic data for the ovine bronchial tree. With the use of an identical parameter set, the volume-filling algorithm has produced airway trees with branching asymmetry appropriate for the human and ovine lung, demonstrating the dependence of the method on the shape of the lung or lobe volume. The modeling approach that has been developed can be applied to any level of detail of the airway tree and into any volume shape for the lung; hence it can be used directly for different individuals or animals and for any number of scan-based airways. The resulting models are subject-specific computational meshes with anatomically consistent geometry, suitable for application in simulation studies.

  8. Rhinovirus infection induces cytotoxicity and delays wound healing in bronchial epithelial cells

    Directory of Open Access Journals (Sweden)

    Constantopoulos Andreas G

    2005-10-01

    Full Text Available Abstract Background Human rhinoviruses (RV, the most common triggers of acute asthma exacerbations, are considered not cytotoxic to the bronchial epithelium. Recent observations, however, have questioned this knowledge. The aim of this study was to evaluate the ability of RV to induce epithelial cytotoxicity and affect epithelial repair in-vitro. Methods Monolayers of BEAS-2B bronchial epithelial cells, seeded at different densities were exposed to RV serotypes 1b, 5, 7, 9, 14, 16. Cytotoxicity was assessed chromatometrically. Epithelial monolayers were mechanically wounded, exposed or not to RV and the repopulation of the damaged area was assessed by image analysis. Finally epithelial cell proliferation was assessed by quantitation of proliferating cell nuclear antigen (PCNA by flow cytometry. Results RV1b, RV5, RV7, RV14 and RV16 were able to induce considerable epithelial cytotoxicity, more pronounced in less dense cultures, in a cell-density and dose-dependent manner. RV9 was not cytotoxic. Furthermore, RV infection diminished the self-repair capacity of bronchial epithelial cells and reduced cell proliferation. Conclusion RV-induced epithelial cytotoxicity may become considerable in already compromised epithelium, such as in the case of asthma. The RV-induced impairment on epithelial proliferation and self-repair capacity may contribute to the development of airway remodeling.

  9. Bronchial stump fistula :treatment with covered retrievable hinged metallic stents-preliminary clinical experience

    Institute of Scientific and Technical Information of China (English)

    LI Yong-dong; HAN Xin-wei; WU Gang; LI Ming-hua

    2007-01-01

    Objective To evaluate the preliminaily clinical efficacy and retrievability of a retrievable hinged covered metallic stent in the treatment of the bronchial stump fistula (BSF). Methods Between April 2003 and March 2005, 8 patients with bronchial stump fistula after pneumonectomy or lobectomy were treated with two types (A and B) of retrievable hinged covered metallic stents. Type A stent was placed in 6 patients and type B in 2 under fluoroscopic guidance. The stent was removed with a retrieval set when BSF was healed or complications occurred. Results Stent placement in the bronchial tree was technically successful in all patients, without procedure-related complications. Immediate closure of the BSF was achieved in all patients after the procedure. Stents were removed from all patients but one. Removal of the stents was difficult in two patients due to tissue hyperplasia. Patients were followed up for 6 - 21 months. Placement of the stents remained stable in all patients except one due to severe cough. Permanent closure of BSF was achieved in 7(87.5%) of 8 patients. Conclusion Use of a retrievable hinged covered expandable metallic stent is a simple,safe, and effective procedure for closure of the BSF. Retrieval of the stent seems to be feasible. (J Intervent Radiol, 2007, 16: 253-257)[ Key words ] Fistula, pulmonary; Bronchialpleural fistula; Stents and prostheses; Computed tomography

  10. Correlation between the severity of critically ill patients and clinical predictors of bronchial aspiration

    Science.gov (United States)

    de Medeiros, Gisele Chagas; Sassi, Fernanda Chiarion; Zambom, Lucas Santos; de Andrade, Claudia Regina Furquim

    2016-01-01

    Objective: To determine whether the severity of non-neurological critically ill patients correlates with clinical predictors of bronchial aspiration. Methods: We evaluated adults undergoing prolonged orotracheal intubation (> 48 h) and bedside swallowing assessment within the first 48 h after extubation. We collected data regarding the risk of bronchial aspiration performed by a speech-language pathologist, whereas data regarding the functional level of swallowing were collected with the American Speech-Language-Hearing Association National Outcome Measurement System (ASHA NOMS) scale and those regarding health status were collected with the Sequential Organ Failure Assessment (SOFA). Results: The study sample comprised 150 patients. For statistical analyses, the patients were grouped by ASHA NOMS score: ASHA1 (levels 1 and 2), ASHA2 (levels 3 to 5); and ASHA3 (levels 6 and 7). In comparison with the other patients, those in the ASHA3 group were significantly younger, remained intubated for fewer days, and less severe overall clinical health status (SOFA score). The clinical predictors of bronchial aspiration that best characterized the groups were abnormal cervical auscultation findings and cough after swallowing. None of the patients in the ASHA 3 group presented with either of those signs. Conclusions: Critically ill patients 55 years of age or older who undergo prolonged orotracheal intubation (≥ 6 days), have a SOFA score ≥ 5, have a Glasgow Coma Scale score ≤ 14, and present with abnormal cervical auscultation findings or cough after swallowing should be prioritized for a full speech pathology assessment. PMID:27167432

  11. The Efficacy of Bronchial Thermoplasty for Severe Persistent Asthma– the First National Experience

    LENUS (Irish Health Repository)

    Watchorn, DC

    2016-05-01

    There is an unmet need for new therapies in severe persistent asthma. Bronchial thermoplasty is a bronchoscopic procedure which employs radiofrequency energy to reduce airway smooth muscle and has been demonstrated to improve symptomatic control in severe persistent asthma in other populations. Seven patients have completed bronchial thermoplasty at a tertiary referral centre in Ireland. Asthma Control Test scores and data on hospitalisations, exacerbations, maintenance corticosteroid requirements, rescue bronchodilator use and peak expiratory flow rate (PEFR) were compared one year before and one year post treatment. Significant improvements were demonstrated in mean Asthma Control Test scores, from 8.9 to 14.7 (p = 0.036). Trends towards improvement were seen in mean hospitalisations (respective values for total in 12 month period 5.0, 0.9; p = 0.059) and PEFR (181.4 l\\/min, 280 l\\/min respectively; p = 0.059). These data support the use of bronchial thermoplasty in severe persistent asthma in the Irish population.

  12. Bronchial hyperreactivity and spirometric impairment in polysensitized patients with allergic rhinitis

    Directory of Open Access Journals (Sweden)

    Tosca Maria A

    2004-03-01

    Full Text Available Abstract Background We previously demonstrated in a group of patients with perennial allergic rhinitis alone impairment of spirometric parameters and high percentage of subjects with bronchial hyperreactivity (BHR. The present study aimed at evaluating a group of polysensitized subjects suffering from allergic rhinitis alone to investigate the presence of spirometric impairment and BHR during the pollen season. Methods One hundred rhinitics sensitized both to pollen and perennial allergens were evaluated during the pollen season. Spirometry and methacholine bronchial challenge were performed. Results Six rhinitics showed impaired values of FEV1 without referred symptoms of asthma. FEF 25–75 values were impaired in 28 rhinitics. Sixty-six patients showed positive methacholine bronchial challenge. FEF 25–75 values were impaired only in BHR positive patients (p Conclusions This study evidences that an impairment of spirometric parameters may be observed in polysensitized patients with allergic rhinitis alone during the pollen season. A high percentage of these patients had BHR. A close relationship between upper and lower airways is confirmed.

  13. Perfusion CT in patients with advanced bronchial carcinomas: a novel chance for characterization and treatment monitoring?

    International Nuclear Information System (INIS)

    Advanced bronchial carcinomas by means of perfusion and peak enhancement using dynamic contrast-enhanced multislice CT are characterized. Twenty-four patients with advanced bronchial carcinoma were examined. During breathhold, after injection of a contrast-medium (CM), 25 scans were performed (1 scan/s) at a fixed table position. Density-time curves were evaluated from regions of interest of the whole tumor and high- and low-enhancing tumor areas. Perfusion and peak enhancement were calculated using the maximum-slope method of Miles and compared with size, localization (central or peripheral) and histology. Perfusion of large tumors (>50 cm3) averaged over both the whole tumor (P=0.001) and the highest enhancing area (P=0.003) was significantly lower than that of smaller ones. Independent of size, central carcinomas had a significantly (P=0.04) lower perfusion (mean 27.9 ml/min/100 g) than peripheral ones (mean 66.5 ml/min/100 g). In contrast, peak enhancement of central and peripheral carcinomas was not significantly different. Between non-small-cell lung cancers and small-cell lung cancers, no significant differences were observed in both parameters. In seven tumors, density increase after CM administration started earlier than in the aorta, indicating considerable blood supply from pulmonary vessels. Tumor perfusion was dependent on tumor size and localization, but not on histology. Furthermore, perfusion CT disclosed blood supply from both pulmonary and/or bronchial vessels in some tumors. (orig.)

  14. Increased polysomy of chromosome 7 in bronchial epithelium from patients at high risk for lung cancer

    Energy Technology Data Exchange (ETDEWEB)

    Belinsky, S.A.; Neft, R.E.; Lechner, J.F. [and others

    1995-12-01

    Current models of carcinogenesis suggest that tissues progress through multiple genetic and epigenetic changes which ultimately lead to development of invasive cancer. Epidemiologic studies of Peto, R.R. and J.A. Doll indicate that the accumulation of these genetic changes over time, rather than any single unique genetic change, is probably responsible for development of the malignant phenotype. The bronchial epithelium of cigarette smokers is diffusely exposed to a broad spectrum of carcinogens, toxicants, and tumor promoters contained in tobacco smoke. This exposure increases the risk of developing multiple, independent premalignant foci throughout the lower respiratory tract that may contain independent gene aberrations. This {open_quotes}field cancerization{close_quotes} theory is supported by studies that have demonstrated progressive histologic changes distributed throughout the lower respiratory tract of smokers. A series of autopsy studies demonstrated that cigarette smokers exhibit premalignant histologic changes ranging from hyperplasia and metaplasia to severe dysplasia and carcinoma in situ diffusely throughout the bronchial mucosa. The proximal bronchi appear to exhibit the greatest number of changes, particularly at bifurcations. The results described are the first to quantitate the frequency for a chromosome aberration in {open_quotes}normal{close_quotes} bronchial epithelial cells.

  15. Translocation of SiO2-NPs across in vitro human bronchial epithelial monolayer

    Science.gov (United States)

    George, I.; Vranic, S.; Boland, S.; Borot, M. C.; Marano, F.; Baeza-Squiban, A.

    2013-04-01

    Safe development and application of nanotechnologies in many fields require better knowledge about their potential adverse effects on human health. Evidence of abilities of nanoparticles (NPs) to cross epithelial barriers and reach secondary organs via the bloodstream led us to investigate the translocation of SiO2 NPs of 50 nm (50 nm-SiO2-NPs) across human bronchial epithelial cells that are primary targets after exposure to inhaled NPs. We quantified the translocation of fluorescently labelled SiO2 NPs at non-cytotoxic concentrations (5 and 10 μg/cm2) across Calu-3 epithelial monolayer. After 14 days in culture Calu-3 cells seeded onto 3 μm-polycarbonate Transwell membranes formed an efficient bronchial barrier assessed by measurement of the transepithelial electric resistance and quantification of the permeability of the monolayer. After 24 hours of exposure, we observed a significant translocation of NPs that was more important when the initial NP concentration decreased. Confocal microscopy observations revealed NP uptake by cells and an important NP retention inside the porous membrane. In conclusion, 50 nm-SiO2-NPs can cross the human bronchial epithelial barrier without affecting the integrity of the epithelial cell monolayer.

  16. Increased polysomy of chromosome 7 in bronchial epithelium from patients at high risk for lung cancer

    International Nuclear Information System (INIS)

    Current models of carcinogenesis suggest that tissues progress through multiple genetic and epigenetic changes which ultimately lead to development of invasive cancer. Epidemiologic studies of Peto, R.R. and J.A. Doll indicate that the accumulation of these genetic changes over time, rather than any single unique genetic change, is probably responsible for development of the malignant phenotype. The bronchial epithelium of cigarette smokers is diffusely exposed to a broad spectrum of carcinogens, toxicants, and tumor promoters contained in tobacco smoke. This exposure increases the risk of developing multiple, independent premalignant foci throughout the lower respiratory tract that may contain independent gene aberrations. This open-quotes field cancerizationclose quotes theory is supported by studies that have demonstrated progressive histologic changes distributed throughout the lower respiratory tract of smokers. A series of autopsy studies demonstrated that cigarette smokers exhibit premalignant histologic changes ranging from hyperplasia and metaplasia to severe dysplasia and carcinoma in situ diffusely throughout the bronchial mucosa. The proximal bronchi appear to exhibit the greatest number of changes, particularly at bifurcations. The results described are the first to quantitate the frequency for a chromosome aberration in open-quotes normalclose quotes bronchial epithelial cells

  17. Effects of budesonide on serum inflammatory factors and immune function in patients with bronchial asthma

    Institute of Scientific and Technical Information of China (English)

    Mi nawa•abasi; Zumulaiti•Tuerxun; Yang Xiao-Hong

    2015-01-01

    Objective:To explore the effects of budesonide on serum inflammatory factors and immune function in patients with bronchial asthma.Methods:A total of 98 cases of bronchial asthma patients were randomly divided into the observation group and the control group according to the order of treatment, each of 49 cases. Patients in control group were treated with conventional treatment. On the basis of this, patients in the observation group were treated with budesonide inhalation. The clinical symptoms and signs, serum level of inflammatory factors and immune function of two groups were observed before and after treatment. Results:After treatment, the serum IL-6, IL-8, IL-17, TNF-alpha levels of observation group were significantly lower than the control group, with significantly difference(P<0.05). After treatment, IgA, IgG, IgM, CD4+, CD8+, and CD4+/CD8+ levels were significantly higher than the control group, with significantly difference (P<0.05).Conclusion:The effect of budesonide on bronchial asthma is good, can effectively improve the symptoms and signs, reduce serum inflammatory factor levels, enhance immune function in patients. It is worthy of promotion and application.

  18. HRCT and bronchial asthma: visualization of the pathophysiologic changes of the pulmonary parenchyma after inhalation provocation; HRCT und Asthma bronchiale: Visualisierung pathophysiologischer Veraenderungen des Lungenparenchyms nach inhalativer Provokation

    Energy Technology Data Exchange (ETDEWEB)

    Schueller, G.; Neumann, K.; Helbich, T.; Herold, C.J. [Univ. Klinik fuer Radiodiagnostik, Abt. fuer konservative Faecher, Vienna (Austria); Riemer, H. [Univ. Klinik fuer Innere Medizin IV, Abt. fuer Pulmologie, Vienna (Austria); Backfrieder, W. [Allgemeines Krankenhaus, Vienna (Austria). Inst. fuer Biomedizinische Technik und Physik; Sertl, K. [Krankenhaus der Stadt Wien-Floridsdorf (Austria). Abt. fuer Innere Medizin; Pittner, B. [Vienna Univ. (Austria). Inst. fuer Medizinische Statistik

    2004-03-01

    Purpose: To characterize parenchymal lung affections morphologically in patients with asthma and healthy subjects by high resolution computed tomography (HRCT) subsequent to histamine-triggered inhalation bronchoprovocation and salbutamolinduced broncholysis, and to compare the results with pulmonary function tests. Materials and Methods: Fifteen asthmatics with bronchial hyperreactivity, with a>20% decrease in FEV1 and a>10 mmHg decrease in PaO{sub 2} after bronchoprovocation (PC20%+), twelve asthmatics with a<20% decrease in FEV1 and a>10 mmHg decrease in PaO{sub 2} after bronchoprovocation (PC20%-), and eight healthy persons without bronchial hyperreactivity underwent inhalation bronchoprovocation and broncholysis. Spirometer-triggered HRCT at high lung volumes was performed, and total and peripheral lung densities and the amount of solid lung structures, representing predominantly vessels, were measured. Results: After bronchoprovocation, we observed significant decreases in total and peripheral lung densities in all groups (p<0.0005), and a significant increase in lung densities subsequent to bronchodilation (p<0.0002). The morphological alterations in solid lung structure were not significantly different after bronchoprovocation or broncholysis (p>0.05), as compared to the baseline measurements. In hyperreactive patients, PaO{sub 2} significantly decreased after provocation and significantly increased after lysis (p<0.05). In PC20%+ asthmatics, a mean reduction of 27.8% in FEV1 was observed, which was <20% in the other groups. No significant correlations were observed between radiological data and the results of pulmonary function tests. In healthy persons, we demonstrated highly significant parenchymal response to bronchoprovocation and broncholysis, which was not otherwise documented by pulmonary function tests. Conclusion: In both PC20%+ and PC20%- patients as well as in healthy individuals, HRCT was efficient in the evaluation of pathoanatomical

  19. Respiratory Diseases in Iron Ore Miners and Millers

    OpenAIRE

    Edstrom, Harry W.

    1989-01-01

    Workers in iron mines are at risk of developing interstitial lung disease if the dust levels are above the threshold limit value. However, they more commonly develop the usual diseases that affect all workers. Some illnesses, such as chronic bronchitis, bronchial asthma, and the collagen vascular diseases that affect the lung, may be more severe because of the inhalation of dust. The most difficult problem is to differentiate asymptomatic sarcoidosis from pneumoconiosis. The family doctor who...

  20. Gene expression profiling and pathway analysis of human bronchial epithelial cells exposed to airborne particulate matter collected from Saudi Arabia

    Energy Technology Data Exchange (ETDEWEB)

    Sun, Hong [Department of Environmental Medicine, NYU School of Medicine, Tuxedo, NY (United States); Shamy, Magdy [Department of Environmental Sciences, Faculty of Meteorology, Environment and Arid Land Agriculture, King Abdulaziz University, Jeddah (Saudi Arabia); Kluz, Thomas; Muñoz, Alexandra B.; Zhong, Mianhua; Laulicht, Freda [Department of Environmental Medicine, NYU School of Medicine, Tuxedo, NY (United States); Alghamdi, Mansour A.; Khoder, Mamdouh I. [Department of Environmental Sciences, Faculty of Meteorology, Environment and Arid Land Agriculture, King Abdulaziz University, Jeddah (Saudi Arabia); Chen, Lung-Chi [Department of Environmental Medicine, NYU School of Medicine, Tuxedo, NY (United States); Costa, Max, E-mail: Max.Costa@nyumc.org [Department of Environmental Medicine, NYU School of Medicine, Tuxedo, NY (United States)

    2012-12-01

    Epidemiological studies have established a positive correlation between human mortality and increased concentration of airborne particulate matters (PM). However, the mechanisms underlying PM related human diseases, as well as the molecules and pathways mediating the cellular response to PM, are not fully understood. This study aims to investigate the global gene expression changes in human cells exposed to PM{sub 10} and to identify genes and pathways that may contribute to PM related adverse health effects. Human bronchial epithelial cells were exposed to PM{sub 10} collected from Saudi Arabia for 1 or 4 days, and whole transcript expression was profiled using the GeneChip human gene 1.0 ST array. A total of 140 and 230 genes were identified that significantly changed more than 1.5 fold after PM{sub 10} exposure for 1 or 4 days, respectively. Ingenuity Pathway Analysis revealed that different exposure durations triggered distinct pathways. Genes involved in NRF2-mediated response to oxidative stress were up-regulated after 1 day exposure. In contrast, cells exposed for 4 days exhibited significant changes in genes related to cholesterol and lipid synthesis pathways. These observed changes in cellular oxidative stress and lipid synthesis might contribute to PM related respiratory and cardiovascular disease. -- Highlights: ► PM exposure modulated gene expression and associated pathways in BEAS-2B cells. ► One-day exposure to PM induced genes involved in responding to oxidative stress. ► 4-day exposure to PM changed genes associated to cholesterol and lipid synthesis.

  1. 支气管哮喘动物模型的研究进展%Research Progress in Animal Model of Bronchial Asthma

    Institute of Scientific and Technical Information of China (English)

    吕洪臻; 黄诚

    2012-01-01

    建立支气管哮喘动物模型有助于对哮喘的研究.针对Th2型细胞驱动的变态反应动物模型研究较多,并已应用于相关药物的研究.对支气管哮喘动物模型有一定疗效的药物对人类患者疗效欠佳,这可能与选择实验的动物和诱导哮喘表型的方法有关.啮齿动物和豚鼠模型是最常使用的支气管哮喘动物模型.目前动物模型仍存在许多问题,如在免疫学和解剖学上动物与人类存在差异,大多数模型在致敏时需要佐剂,成年动物应用较多.因此,明确每种模型的相对优点和局限性十分必要.%Animal models of bronchial asthma are beneficial to the study of asthma. These models have highlighted the importance of T-helper type 2 driven allergic responses in the progression of asthma and have been useful in the identification of interrelated drugs. Many drugs that have been shown to have some efficacy in animal models of bronchial asthma have shown little clinical benefit in human asthmatics, which may be due to a number of factors including the species of animal chosen and the methods used to induce an asthmatic phenotype in animals. Rodents and guinea-pigs are the most popular bronchial asthma models. At present, there are a lot of problems in animal models of asthma, for example the difference in immunology and anatomy between these species and human, the requirement for adjuvant during sensitization in most models, adult animals as the primary disease model. Therefore understanding of the relative merits and limitations of each model is necessary.

  2. Anaesthesia for children with bronchial asthma and respiratory infections

    Directory of Open Access Journals (Sweden)

    M C Rajesh

    2015-01-01

    Full Text Available Asthma represents one of the most common chronic diseases in children with an increasing incidence reported worldwide. The key to successful anaesthetic outcome involves thorough pre-operative assessment and optimisation of the child's pulmonary status. Judicious application of proper anti-inflammatory and bronchodilatory regimes should be instituted as part of pre-operative preparation. Bronchospasm triggering agents should be carefully probed and meticulously avoided. A calm and properly sedated child at the time of induction is ideal, so also is extubation in a deep plane with an unobstructed airway. Wherever possible, regional anaesthesia should be employed. This will avoid airway manipulations, with additional benefit of excellent peri-operative analgesia. Agents with a potential for histamine release and techniques that can increase airway resistance should be diligently avoided. Emphasis must be given to proper post-operative care including respiratory monitoring, analgesia and breathing exercises.

  3. [The application of "preventive treatment theory" in chronic airway inflammatory disease].

    Science.gov (United States)

    Dong, Jing-Cheng; Liu, Bao-Jun; Zhang, Hong-Ying

    2013-07-01

    Bronchial asthma and chronic obstructive pulmonary disease (COPD), as chronic airway inflammatory diseases, seriously threaten the health of human beings. Chinese medicine has obvious advantages in prevention and treatment of them. "Preventive treatment theory" is a sort summarization of preventive medicine in Chinese medicine. The theory is not only reflected at the disease prevention levels, also embodied in the active treatment and the rehabilitation process. It was especially deep and colorfully embodied in the prevention and treatment of chronic airway inflammatory diseases such as asthma and COPD. In this paper,clarified were the prevention and treatment targets, ways of thinking and methods in different stages of asthma and COPD from various viewpoints including prevention before disease occurrence, treating disease at disease onset, preventing the aggravation once disease occurs, and consolidation after disease occurs. We hope to improve ways of thinking and prevention and treatment levels of bronchial asthma and COPD by Chinese medicine. PMID:24063226

  4. Plasma Adiponectin Concentrations and Adiponectin Gene Polymorphisms Are Associated with Bronchial Asthma in the Chinese Li Population

    Directory of Open Access Journals (Sweden)

    Yipeng Ding

    2015-10-01

    Full Text Available The purpose of this study was to determine the clinical significance of changes in the plasma  adiponectin  concentration  in  patients  with  bronchial  asthma  and  to  test  the association between the single nucleotide polymorphisms (SNPs rs2241766 and rs1501299 in the ADIPOQ gene and bronchial asthma in the Chinese Li population.We selected 120 cases and 120 controls, and plasma adiponectin, interleukin (IL-6, and tumor  necrosis  factor-alpha  (TNF-α  levels  were  measured  by  enzyme-linked immunosorbent assay (ELISA. In addition, we genotyped two tag single nucleotide polymorphisms (tSNPs and evaluated their association with bronchial asthma using the χ2 test and genetic model analysis.Compared to controls, patients with acute exacerbation of bronchial asthma showedsignificantly lower adiponectin and significantly higher IL-6 and TNF-α levels (p<0.01. Apositive association was found between the rs1501299 SNP and acute exacerbation (OR =1.62; 95% CI= 1.08-2.43; p= 0.019.The inverse correlation between the plasma adiponectin concentration and asthma exacerbation indicates that adiponectin may play a protective role in the pathogenesis of asthma. Meanwhile, our findings suggest that ADIPOQ polymorphisms influence the risk of developing bronchial asthma in Chinese Li population.

  5. Efectividad de la ozonoterapia en un modelo experimental de hipersensibilidad bronquial en cobayo - Effectiveness of ozonetherapy in an experimental model of bronchial hyprereactivity in guinea pig

    Directory of Open Access Journals (Sweden)

    Zayas, Kenia

    2009-12-01

    Full Text Available Resumenestudio de un gran número de especialistas en todo el mundo por laalta incidencia de muertes que ocasiona y los elevados costoshospitalarios que su atención demanda. Se conoce que el ozonoaplicado por vías y dosis adecuadas, produce acciones biológicascon efectos terapéuticos sin causar reacciones adversas. Esto haceposible su empleo en una gran variedad de procesos patológicoscomo en el asma. En este trabajo se estudió la presiónintrabronquial (PIB, IgG y la actividad de la mieloperoxidasa (MPOen 5 grupos experimentales tratados con la mezcla ozono/oxigeno(MOO (0,2 y 1,2 mg/kg y oxigeno (O2 (7 mL, donde un grupofue utilizado en el control de las variables estudiadas y al resto se le aplicó un esquema de hipersensibilidad bronquial con ovoalbúmina.Transcurridas 24 h de la ultima aplicación de la MOO, sedeterminaron los parámetros siguientes: Presión intrabronquial(PIB, niveles de IgG en el suero y la actividad de lamieloperoxidasa (MPO en tejido pulmonar y el análisishistopatológico del tejido. Los resultados mostraron unadisminución significativa de la PIB, en los niveles de IgG en suero yde la actividad de la MPO en el tejido pulmonar de los animalestratados con la MOO. Histopatológicamente se confirmó una disminución en el infiltrado de neutrófilos en el tejido bronquial de animales tratados con la MOO.SummaryBronchial asthma is a disease which is currently studied by manyspecialists in medicine because is causative of a high morbidity andmortality accompanied of high cost of hospitalization and requiredmedical assistance. It is well known that administration of lowconcentration of ozone in mixture with oxygen by adequate routesproduces biological actions with therapeutic effects and withoutconcomitant adverse reactions. It allows the use of ozone/oxygenmixture (OOM in various diseases such as bronchial asthma. In thisresearch male guinea pigs were used, which were divided in fiveexperimental groups. The animals

  6. Assessment of life quality in patients with bronchial asthma residing in Krakow in the areas of varying concentrations of particulate matter (PM10

    Directory of Open Access Journals (Sweden)

    Monika Ścibor

    2015-03-01

    Full Text Available Introduction. Asthma is a chronic disease, from which more and more people in the world suffer. It is connected with many bothersome symptoms and limitations, which result in decreased quality of life for the patient. Environmental and individual aspects do not necessarily affect individuals in the same way, so it is necessary to determine which factors have predominantly impacted on an individual, in order to minimize their impact and to take better control over treatment of asthma. The aim of this research was to compare the quality of life among patients with bronchial asthma living in Krakow in the areas where they get exposed to varying concentrations of particulate matter (PM10. Material and methods. The study included 98 adults diagnosed with bronchial asthma. The research was conducted using the AQLQ poll. PM10 concentration was measured in several Malopolska Air Pollution Monitoring Stations located throughout the city. Results. Analyzing the quality of life in the view of symptoms, activity limitations and emotional well being, there was a substantial statistical difference observed in people occupying the areas with different PM10 concentrations. No significant statistical difference was observed in the frequency of asthma symptoms caused by the environmental stimuli between the 2 discussed groups. One group of patients who came to the allergy clinic for control of asthma symptoms and the second group who live in the vicinity of the monitoring stations measuring PM10 concentrations. Conclusions. For many of the cases, the quality of life was not worse for patients with asthma living in an area with slightly elevated concentrations of PM10, and sometimes paradoxically the quality of life was improved. These results show that PM10 concentrations do not correlate with quality of life of asthma patients.

  7. Obstructive pulmonary disease

    International Nuclear Information System (INIS)

    Although /sup 133/xenon ventilation studies and aerosol inhalation images have been shown to be more sensitive than other measurements of pulmonary function in detecting early airway disease, they fall short as ideal screening tests. In addition they do not distinguish between the different diseases of air flow obstruction. The diagnosis of the conditions discussed in this paper does not require studies of regional lung function. It is doubtful if further refinements in instrumentation or the introduction of other radioisotopes would really help in this matter. Chronic bronchitis, emphysema, and bronchial asthma are the major obstructive pulmonary disease. Cystic fibrosis and bronchiectasis, which are less common, are also accompanied by airway obstruction. Local obstruction of a bronchus may also occur as a result of a tumor or foreign body or a mucous plug

  8. Cystic fibrosis bronchial epithelial cells are lipointoxicated by membrane palmitate accumulation.

    Directory of Open Access Journals (Sweden)

    Laurie-Anne Payet

    Full Text Available The F508del-CFTR mutation, responsible for Cystic Fibrosis (CF, leads to the retention of the protein in the endoplasmic reticulum (ER. The mistrafficking of this mutant form can be corrected by pharmacological chaperones, but these molecules showed limitations in clinical trials. We therefore hypothesized that important factors in CF patients may have not been considered in the in vitro assays. CF has also been associated with an altered lipid homeostasis, i. e. a decrease in polyunsaturated fatty acid levels in plasma and tissues. However, the precise fatty acyl content of membrane phospholipids from human CF bronchial epithelial cells had not been studied to date. Since the saturation level of phospholipids can modulate crucial membrane properties, with potential impacts on membrane protein folding/trafficking, we analyzed this parameter for freshly isolated bronchial epithelial cells from CF patients. Interestingly, we could show that Palmitate, a saturated fatty acid, accumulates within Phosphatidylcholine (PC in CF freshly isolated cells, in a process that could result from hypoxia. The observed PC pattern can be recapitulated in the CFBE41o(- cell line by incubation with 100 µM Palmitate. At this concentration, Palmitate induces an ER stress, impacts calcium homeostasis and leads to a decrease in the activity of the corrected F508del-CFTR. Overall, these data suggest that bronchial epithelial cells are lipointoxicated by hypoxia-related Palmitate accumulation in CF patients. We propose that this phenomenon could be an important bottleneck for F508del-CFTR trafficking correction by pharmacological agents in clinical trials.

  9. MECHANISMS OF COUNTERACTING FLAP-VALVE BRONCHIAL OBSTRUCTION IN CASE OF OBSTRUCTIVE PULMONARY EMPHYSEMA

    Directory of Open Access Journals (Sweden)

    K. F. Tetenev

    2015-01-01

    Full Text Available The research goal was to formulate and substantiate the hypothesis explaining support for an expiratory air flow in case of pulmonary emphysema. The research method consisted in comparing the mechanical properties of lungs in practically healthy individuals (37 individuals, mean age – (30.4 ± 1.7 y.o. and COPD patients with pronounced lung emphysema (30 patients, mean age – (52.1 ± 2.3 y.o. as well as those of isolated normal lungs (n = 14 and isolated lungs of patients who died of COPD (n = 5. Pulmo-nary mechanics was studied via the simultaneous measurement of transpulmonary pressure and lung ven-tilation volume. General lung hysteresis and elastic lung hysteresis were calculated. The mechanical properties of isolated lungs were studied using passive ventilation under the Donders bell. The air flow was interrupted in order to measure alveolar pressure and develop an elastic lung hysteresis curve. Pres-sure in the Donders bell was changed by means of a special pump in automatic and manual modes. The research has not revealed any fundamental differences between the mechanical properties of the normal and emphysematous lungs. A minimum increase in the pressure inside the Donders bell over atmospheric pressure used to stop air ejection in both normal and the emphysematous lungs as the result of flap-valve bronchial obstruction. In living beings, air is ejected from lungs with an increase in pressure under the conditions of forced expiration. Pressure increases up to (38.6 ± 2.71 cm H2O in healthy individuals and up to (20.5 ± 1.86 cm H2O in COPD patients. Probably, an expiratory air flow is supported by active expiratory bronchial dilatation that counteracts flap-valve bronchial obstruction. The hypothesis is based on the confirmed ability of the lungs to perform inspiratory actions (in addition to the action of respiratory muscles and the theory of mechanical lung activity.

  10. Local C-Reactive Protein Expression in Obliterative Lesions and the Bronchial Wall in Posttransplant Obliterative Bronchiolitis

    Directory of Open Access Journals (Sweden)

    Outi E. Päiväniemi

    2009-01-01

    Full Text Available The local immunoreactivity of C-reactive protein (CRP was studied in a heterotopic porcine model of posttranplant obliterative bronchiolitis (OB. Bronchial allografts and control autografts were examined serially 2–28 days after subcutaneous transplantation. The autografts stayed patent. In the allografts, proliferation of inflammatory cells (P<.0001 and fibroblasts (P=.02 resulted in occlusion of the bronchial lumens (P<.01. Influx of CD4+ (P<.001 and CD8+ (P<.0001 cells demonstrated allograft immune response. CRP positivity simultaneously increased in the bronchial walls (P<.01, in macrophages, myofibroblasts, and endothelial cells. Local CRP was predictive of features characteristic of OB (R=0.456–0.879, P< .05−P<.0001. Early obliterative lesions also showed CRP positivity, but not mature, collagen-rich obliterative plugs (P<.05. During OB development, CRP is localized in inflammatory cells, myofibroblasts and endothelial cells probably as a part of the local inflammatory response.

  11. Application of the autoblood treated by preliminary extracorporeal X-ray irradiation in the therapy of bronchial asthma patients

    International Nuclear Information System (INIS)

    A therapeutic method including extracorporeal x-ray irradiation of the autoblood of patients with bronchial asthma complicated by cortisone dependence and polyvalent drug intolerance, has been elaborated and clinically tested. The use of this method brings about good short-term results in 90% of cases and good long-term results in 40% of cases (14-38 months). It provides an opportunity to give up corticosteroids in more than 60% of patients and to decrease the hormone dose in the rest of cortisone-dependent patients with bronchial asthma. The above therapeutic method can be used as an independent type of treatment, especially in the presence of polyvalent drug allergy and as an element of multiple modality therapy of bronchial asthma patients. The method was used under in-patient conditions only. No side effects were marked in the course of the clinical trial. To carry out this type of therapy, patients should be thoroughly screened

  12. Effect of oral preparation of astragalus membranaceous on serum SOD levels in aged patients with chronic bronchial asthma

    International Nuclear Information System (INIS)

    Objective: To investigate the therapeutic effect of oral liquid preparation of astragalus membranaceous in aged patients with chronic bronchial asthma with special reference on the serum SOD levels. Methods: Serum SOD levels were measured with RIA in 42 aged patients with chronic bronchial asthma both before and after a course of treatment with oral liquid preparation of astragalus membranaceous (10ml b. i. d for 3 months) as well as in 35 controls. Results: The patients general condition was greatly improved after the treatment. Before treatment, the serum SOD levels in the patients were significantly lower than those in controls (P 0.05). Conclusion: Oral liquid preparation of astragalus membranaceous was therapeutically useful for chronic bronchial asthma in aged patients with correction of the serum SOD levels. (authors)

  13. Clinical application of radiofrequency ablation combined with bronchial artery infusion of docetaxel in treating non-small cell lung cancer

    International Nuclear Information System (INIS)

    Objective: To discuss the clinical application of radiofrequency ablation combined with bronchial artery infusion of docetaxel in treating non-small cell lung cancer and to summarize the experience of using this therapy in clinical practice. Methods: Radiofrequency ablation was performed in twenty-one patients with lung cancer. The diagnosis was confirmed by CT-guided percutaneous needle biopsy or bronchoscopic biopsy in all patients. One week after radiofrequency ablation treatment, bronchial artery infusion of docetaxel was conducted. The therapeutic results were observed and evaluated. Results: After the treatment, the lesion's size was markedly reduced and the clinical symptoms were dramatically improved in all patients. Conclusion: Radiofrequency ablation combined with bronchial artery infusion of docetaxel is a safe, effective and simple technique with excellent therapeutic results for the treatment of non-small cell lung cancer. It is really worth popularizing this technique in clinical practice. (authors)

  14. Y90-Radioembolization of Lung Metastases via the Bronchial Artery: A Report of 2 Cases

    Energy Technology Data Exchange (ETDEWEB)

    Ricke, Jens, E-mail: jens.ricke@med.ovgu.de; Grosser, Oliver; Amthauer, Holger [Universitaetsklinikum Magdeburg AoeR, Klinik fuer Radiologie und Nuklearmedizin (Germany)

    2013-12-15

    We report successful Y90-radioembolization of the lung applying Y90-resin microspheres via the bronchial artery in two patients with diffuse colorectal and renal cancer metastases, respectively. The deposition of radioactive Y90-resin-microspheres was technically feasible and resulted in a reasonable absorbed dose in the tumors. However, open questions remain regarding dosimetry and safe dose exposure to the lung. The technique warrants thorough investigation before clinical use. A Phase I trial to evaluate safety and preliminary efficacy is under preparation.

  15. [Oxidative stress in pathogenesis of bronchial asthma: a method of correction by inhalation of phospholipid nanoparticles].

    Science.gov (United States)

    Lisitsa, A V; Soodaeva, S K; Klimanov, I A; Aver'ianov, A V

    2014-01-01

    The authors present the results of a prospective simple blind randomized placebo-controlled study for the evaluation of dynamics of biomarkers of oxidative stress (total concentration of nitrate- and nitrite-anions in condensed exhaled breath and plasma, pH of exhaled breath, total antioxidative activity of plasma in patients with bronchial asthma inhaling phospholipid nanoparticles. The results suggest significant positive effect of proposed therapy on dynamics of the main parameters of oxidative stress including reduced concentration of nitric oxide metabolites and increased total antioxidative activity of plasma. No clinically significant reactions were documented.

  16. MicroRNA-221 modulates RSV replication in human bronchial epithelium by targeting NGF expression.

    Directory of Open Access Journals (Sweden)

    Sreekumar Othumpangat

    Full Text Available BACKGROUND: Early-life infection by respiratory syncytial virus (RSV is associated with aberrant expression of the prototypical neurotrophin nerve growth factor (NGF and its cognate receptors in human bronchial epithelium. However, the chain of events leading to this outcome, and its functional implications for the progression of the viral infection, has not been elucidated. This study sought to test the hypothesis that RSV infection modulates neurotrophic pathways in human airways by silencing the expression of specific microRNAs (miRNAs, and that this effect favors viral growth by interfering with programmed death of infected cells. METHODOLOGY: Human bronchial epithelial cells infected with green fluorescent protein-expressing RSV (rgRSV were screened with multiplex qPCR arrays, and miRNAs significantly affected by the virus were analyzed for homology with mRNAs encoding neurotrophic factors or receptors. Mimic sequences of selected miRNAs were transfected into non-infected bronchial cells to confirm the role of each of them in regulating neurotrophins expression at the gene and protein level, and to study their influence on cell cycle and viral replication. PRINCIPAL FINDINGS: RSV caused downregulation of 24 miRNAs and upregulation of 2 (p<0.01. Homology analysis of microarray data revealed that 6 of those miRNAs exhibited a high degree of complementarity to NGF and/or one of its cognate receptors TrKA and p75(NTR. Among the selected miRNAs, miR-221 was significantly downregulated by RSV and its transfection in bronchial epithelial cells maximally inhibited gene and protein expression of NGF and TrKA, increased apoptotic cell death, and reduced viral replication and infectivity. CONCLUSIONS/SIGNIFICANCE: Our data suggest that RSV upregulates the NGF-TrKA axis in human airways by silencing miR-221 expression, and this favors viral replication by interfering with the apoptotic death of infected cells. Consequently, the targeted delivery of

  17. Recurrent Massive Hemoptysis due to Postbronchotomy Bronchial Artery Aneurysm: A Case Report

    Directory of Open Access Journals (Sweden)

    Achilleas Lioulias

    2008-01-01

    Full Text Available Bronchial artery aneurysm (BAA is a rare clinical entity. A case of intrapulmonary BAA associated with previous bronchotomy at the same site is described. A 22-year-old woman, who had undergone bronchotomy of the intermediate bronchus for the removal of a foreign body four years previously, presented with recurrent hemoptysis. Because of an increased risk for spinal cord ischemia, she immediately underwent lung resection rather than therapeutic embolization. The incidental finding of a BAA of any cause cannot be assumed to be stable, and immediate management should be undertaken regardless of the presence or absence of symptoms.

  18. Allyl Isothiocyanate Increases MRP1 Function and Expression in a Human Bronchial Epithelial Cell Line

    OpenAIRE

    Dian-lei Wang; Chen-yin Wang; Yin Cao; Xian Zhang; Xiu-hua Tao; Li-li Yang; Jin-pei Chen; Shan-shan Wang; Ze-geng Li

    2014-01-01

    Multidrug resistance-associated protein 1 (MRP1), a member of the ATP-binding cassette (ABC) superfamily of transporters, plays an important role in normal lung physiology by protecting cells against oxidative stress and toxic xenobiotics. The present study investigates the effects of allyl isothiocyanate (AITC) on MRP1 mRNA and MRP1 protein expression and transporter activity in the immortalised human bronchial epithelial cell line 16HBE14o-. MRP1 mRNA and MRP1 protein expression in 16HBE14o...

  19. Adrenal function in children with bronchial asthma treated with beclomethasone dipropionate or budesonide

    DEFF Research Database (Denmark)

    Bisgaard, H; Damkjaer Nielsen, M; Andersen, B;

    1988-01-01

    The effect of inhaled beclomethasone dipropionate and budesonide on the adrenal function was studied in 30 children (aged 7 to 15 years) with mild bronchial asthma. The trial was designed as a prospective double-blind parallel study of the effect of stepwise increase of either beclomethasone...... difference was found between the two topical steroids (probability value 5.3%), and yet the suppression was apparent in the group of children treated with beclomethasone dipropionate but not in the group of children treated with budesonide. Further studies are desirable in order to ascertain whether...

  20. Emotional aspects of bronchial asthma in Indian patients: Evaluation of an interventional strategy

    Directory of Open Access Journals (Sweden)

    Behera D

    2006-01-01

    Full Text Available In order to improve the self care abilities of the patients with bronchial asthma a ′Self Care Manual′ (a Booklet of 26 pages on bronchial asthma was prepared. An interview schedule was developed to evaluate the usefulness of the manual. The validity and reliability of both these instruments were established. Five hundred and twenty three diagnosed patients of bronchial asthma (260 in the study group to whom the self care manual was given and 263 controls to whom no specific instructions were given were included in the study. Emotions like ′tension′, ′excessive laughter′, ′sadness′, ′happiness′ and ′anger′ etc. that triggered the symptoms were studied. The effect of self care manual on other emotions (FNx01a total of 8 items like ′anxiety due to the disease′, ′tension of taking medicine′ and ′fear of death′ etc. was also studied. Both groups were followed up at 2 weeks, 6 months and 1 year while the same interview schedule was administered on each visit. It was observed that the emotion scores decreased significantly in both the groups (8.08±5.5, 5.89±4.88, 1.44±4.63 and 1.19±4.01 in the study patients, whereas 8.50±6.30, 7.88±6.21, 7.35±6.03 and 5.97±5.81 in the controls at 0 weeks, 2 weeks, 6 months and 1 year respectively. The emotion scores were significantly less in the study group as compared to controls (unpaired ′t′ = 3.57, 8.52 and 7.67 at 2 weeks, 6 months and 1 year respectively (p< 0.001 Multiple logistic regression analysis showed that the odds ratio of study group patients showing significant improvement in emotion scores was 3.34 (95% CI, 1.78-6.25 and 4.26 (95% CI, 2.34-7.63 at 6 months and 1 year respectively as compared to controls. We concluded that patient education (self care manual in the form of a booklet made significant improvement in the emotions in patients with bronchial asthma.

  1. Naturally Occurring Variants of Human A9 Nicotinic Receptor Differentially Affect Bronchial Cell Proliferation and Transformation

    OpenAIRE

    Chikova, Anna; Grando, Sergei A.

    2011-01-01

    Isolation of polyadenilated mRNA from human immortalized bronchial epithelial cell line BEP2D revealed the presence of multiple isoforms of RNA coded by the CHRNA9 gene for α9 nicotinic acetylcholine receptor (nAChR). BEP2D cells were homozygous for the rs10009228 polymorphism encoding for N442S amino acid substitution, and also contained mRNA coding for several truncated isoforms of α9 protein. To elucidate the biologic significance of the naturally occurring variants of α9 nAChR, we compare...

  2. Uncertainties associated with bronchial brachytherapy; Incertidumbres asociadas a la brquiterapia de bronquio

    Energy Technology Data Exchange (ETDEWEB)

    Richarte, J. M.; Lupiani Castellanos, J.; Quinones Rodriguez, L. A.; Ramos Caballero, L. J.; Angulo Pain, E.; Iborra Oquendo, M.; Ramirez, I.; Urena Llinares, A.

    2011-07-01

    Bronchial brachytherapy treatments are affected by movement (breathing, coughing, patient discomfort, etc ...) of the probe lodged in the trachea. From the CT images, this movement results in an uncertain trajectory of the probe according to the reconstruction of the catheter is performed. To establish whether this influence has important implications for the location and distance of the section of bronchus treated, is the goal of this work. Other circumstances, such as inaccuracy in the determination of the probe or the delimitation of volumes, increase the uncertainty in the record that the treatment should be pursued.

  3. 青少年哮喘患者生活质量与焦虑、抑郁的关系%Correlation between quality of life, anxiety and depression in juveniles with bronchial asthma

    Institute of Scientific and Technical Information of China (English)

    王玉; 赵明; 姜丹丹; 郑丽新

    2012-01-01

    目的 探讨青少年哮喘患者生活质量与其焦虑、抑郁的相关性.方法 调查在我院诊治过的120 例青少年哮喘患者及110 例健康对照者,采用自制一般资料调查表、哮喘生活质量调查问卷、焦虑自评量表、抑郁自评量表进行数据收集.结果 哮喘患者生活质量总体情况较差,影响因素依次为病程、性别、过去6 个月有无发生过重度哮喘;焦虑与生活质量(P<0.05)、情感(P<0.01) 及总分(P<0.05) 存在相关性.结论 青少年哮喘患者生活质量较差,病程长、女性及过去6 个月发生重度哮喘是影响生活质量的危险因素,焦虑、抑郁等负性情绪与生活质量相互影响.%Objective To study the correlation between quality of life, anxiety and depression in juveniles with bronchial asthma. Methods Clinical data about 118 juveniles with bronchial asthma admitted to our hospital and 110 healthy controls were collected with self-designed questionnaires of general data and quality of life, self-rating anxiety scale, and self-rating depression scale. Results The quality of life in juveniles with bronchial asthma was rather poor. Its influencing factors were disease course, gender, and no occurrence of severe asthma in the past 6 months. Anxiety was correlated with the quality of life, emotion and total score(P<0.05). Conclusion The quality of life in juveniles with bronchial asthma is rather poor. Long disease course, female, and occurrence of severe asthma in the past 6 months are the risk factors for the quality of life. Anxiety, depression and quality of life may influence each other.

  4. Bronchial stump aspergillosis after lobectomy for lung cancer as an unusual cause of false positive fluorodeoxyglucose positron emission tomography and computed tomography: a case report

    Directory of Open Access Journals (Sweden)

    Font Albert

    2011-02-01

    Full Text Available Abstract Introduction Bronchial stump aspergillosis is a rare entity characterized by cough and hemoptysis. Case presentation We report the case of a 58-year-old Caucasian woman who developed bronchial stump aspergillosis two years after a left upper lobe resection for lung cancer. Bronchial stump aspergillosis was diagnosed as a result of a focus of increased fluorodeoxyglucose activity in a follow-up positron emission tomography and computed tomography scan. She was treated with oral antifungal therapy and presented with good evolution after three months of treatment. Conclusion Bronchial stump aspergillosis is an unusual complication after pulmonary resection. Clinicians should be aware of it when a local recurrence of cancer around the bronchial stump is suspected based on a positive positron emission tomography and computed tomography finding.

  5. Multislice helical CT analysis of small-sized airway wall thickness in smokers and patients with bronchial asthma

    International Nuclear Information System (INIS)

    There is accumulating evidence that airway remodeling, which contributes to airway narrowing, plays a role in the pathogenesis of bronchial asthma (BA) and chronic obstructive pulmonary disease (COPD). Development of the multislice helical CT (MSCT) with improved spatial resolution has made it possible to obtain more precise imaging of small-sized airways. Small-sized airway wall-thickness was measured using the MSCT scan to analyze small-sized airways of smokers and BA patients, and examine the effects of a β2 agonists on small-sized airway wall-thickness of BA patients. Thirty-six non-asthmatics who participated in the Health Check Program of Iwate Medical University and 25 patients with asthma were recruited. Amongst the 36 non-asthmatics were 20 healthy never-smokers and 15 smokers. The other 25 asthmatics were recruited from the outpatient clinic at Iwate Medical University. MSCT was performed and the right B10 bronchus was chosen for dimensional analysis. Airway wall thickness was expressed as a percentage of wall area (WA%). WA% of the 7 asthmatics before and 30 mim after procaterol (20μg) inspiration were compared. Small-sized airway wall thickness was significantly increased in smokers and patients with asthma compared to healthy never-smokers, when determined by MSCT. Both %V50 and %V25 had significant negative correlations with WA% among the healthy never-smokers and smoker population. Procaterol inspiration reduced WA% in the small airway of patients with asthma. Increase of small-sized airway thickness measured by MSCT scan may reflect peripheral obstructive lesions of smokers and BA patients. (author)

  6. Bronchial reactivity to inhaled histamine and annual rate of decline in FEV1 in male smokers and ex-smokers

    OpenAIRE

    Taylor, R. G.; Joyce, H; E. Gross; Holland, F.; Pride, N. B.

    1985-01-01

    We examined the relations between bronchial reactivity, baseline FEV1, and annual decline of height corrected FEV1 (delta FEV1/ht3) over 7.5 years in 227 men (117 smokers, 71 ex-smokers, and 39 non-smokers). Men with a clinical diagnosis of asthma or receiving bronchodilator treatment were excluded. Bronchial reactivity was determined as the provocation concentration (PC20) of inhaled histamine sufficient to reduce FEV1 by 20%; subjects were divided into reactors (PC20 less than or equal to 1...

  7. Cushing’s like syndrome in typical bronchial carcinoid a case report and review of the literature

    Directory of Open Access Journals (Sweden)

    Ilaria Pedicelli

    2016-01-01

    Full Text Available Cushing’s syndrome occurred in 1–5% of cases of bronchial carcinoids. In this paper we describe a case of typical bronchial carcinoid in a nonsmoker young male with clinical manifestations mimicking a Cushing’s syndrome. The patient performed chest radiograph and computed tomography. Fiberoptic bronchoscopy revealed the presence of an endobronchial mass occluding the bronchus intermedius. A rigid bronchoscopy was necessary for the conclusive diagnosis and for partial resection of the intraluminal tumor. Despite of the presence of Cushingoid features, the normal blood levels of ACTH and cortisol excluded the coexistence of a Cushing’s syndrome.

  8. [Comparative analysis of PD25-V50 and PD20-FEV1 as a judgement of bronchial provocation test].

    Science.gov (United States)

    Yao, W Z; Zhoa, M W; Han, R X

    1994-08-01

    PD25-V50 as a judgement of bronchial provocation test was compared with PD20-FEV1. Both PD25-V50 and PD20-FEV1 showed linear correlation, but PD25-V50 was more sensitive than PD20-FEV1 for the diagnosis of bronchial asthma, and had higher specificity and sensitivity, and lower false-negative and false-positiverate. Moreover PD25-V50 provided the advantages of shorter period of histamine provocation and lower dose of inhaled histamine, so that avoided the side effects of higher dose of histamine.

  9. 支气管哮喘的遗传多因素分析%Analysis of Genetic Factors of Bronchial Asthma

    Institute of Scientific and Technical Information of China (English)

    刘源; 徐捷; 高福生; 刘长云

    2015-01-01

    [ ABSTRACT] Objective To investigate the genetic pattern and screen its relative risk factors of bronchial asthma,and to evaluate the relative importance of the major risk factors for the disease.Methods One hundred cases of bronchial asthma were selected from the out-patient and inpatient departments of pediatrics from the affiliated hospital of weifang medical university.Falconer regression method and Li-Mantel-Cart method were used to estimate the degree of inheritance and separation of bronchial asthma.The survey data were transformed into hierarchical grouping data in order to facilitate the information input,and all information was entered into the SPSS 21 data window for statisti-cal estimation.All the factors were tested by chi-square test,the factors affecting the incidence of asthma were selected,and the main risk fac-tors were screened out by Logistic regression analysis.Results The degree of inheritance of the first degree relatives of bronchial asthma was 84.05%,and the degree of inheritance of the two relatives was 68.84%,and the average weight of one or two degree relatives was 78.87%±6.99%.The separation ratio is less than 0.25.During our tests,we found that the incidence of asthma and these factors has statistical sig-nificance,including family asthma,allergies,eczema,and recurrent respiratory tract infections.Conclusion Bronchial asthma is a multi gene genetic disease,the separation ratio is 0.163.To avoid allergies,eczema,mycoplasma infection can prevent the occurrence of bronchial asth-ma.%目的:探讨支气管哮喘的遗传方式及筛选有关其危险因素,评估各主要危险因素对发病的相对重要性,为哮喘的三级预防提供理论依据。方法选取潍坊医学院附属医院儿科门诊及住院的100例支气管哮喘患儿。通过Falconer回归法和Li-Mantel-Cart法估计支气管哮喘的遗传度和分离比。将调查资料转化为等级分组资料以便于信息录入,将以上信息输入SPSS 21数

  10. The role of autophagy in the pathogenesis of bronchial asthma%自噬在支气管哮喘发病机制中的作用

    Institute of Scientific and Technical Information of China (English)

    曹足; 谭洪毅; 潘频华

    2015-01-01

    自噬是一种防御和应激调控机制.细胞可以通过自噬调节其生物活性及功能.近年来研究发现,自噬参与代谢性疾病、神经退行性病变、感染、免疫和肿瘤等疾病的发生发展.支气管哮喘是一种具有明显家族聚集倾向的多基因遗传性疾病,它的发生受遗传因素和环境因素的双重影响.自噬主要在固有免疫及获得性免疫、病原体感染、遗传因素等支气管哮喘发病机制中起重要作用.%Autophagy is a defense and stress regulatory mechanism.Cells can adjust its biological activity and function trough autophagy.Recently people found that autophagy has an important role in the development of metabolic disease,neurological degenerative diseases,infections,immunity and tumor and so on.Bronchial asthma (asthma) is a kind of familial and polygenic hereditary disease,affected by genetic and environmental factors.Autophagy play an important role in the pathogenesis of asthma mainly in terms of innate and acquired immunity,pathogen infection,genetic.

  11. Lung cancer perfusion: can we measure pulmonary and bronchial circulation simultaneously?

    Energy Technology Data Exchange (ETDEWEB)

    Yuan, Xiaodong; Ao, Guokun; Quan, Changbin; Tian, Yuan; Li, Hong [Hospital of Chinese People' s Liberation Army, Department of Radiology, Beijing (China); Zhang, Jing [Tongji Hospital of Tongji University, Department of Radiology, Shanghai (China)

    2012-08-15

    To describe a new CT perfusion technique for assessing the dual blood supply in lung cancer and present the initial results. This study was approved by the institutional review board. A CT protocol was developed, and a dual-input CT perfusion (DI-CTP) analysis model was applied and evaluated regarding the blood flow fractions in lung tumours. The pulmonary trunk and the descending aorta were selected as the input arteries for the pulmonary circulation and the bronchial circulation respectively. Pulmonary flow (PF), bronchial flow (BF), and a perfusion index (PI, = PF/ (PF + BF)) were calculated using the maximum slope method. After written informed consent was obtained, 13 consecutive subjects with primary lung cancer underwent DI-CTP. Perfusion results are as follows: PF, 13.45 {+-} 10.97 ml/min/100 ml; BF, 48.67 {+-} 28.87 ml/min/100 ml; PI, 21 % {+-} 11 %. BF is significantly larger than PF, P < 0.001. There is a negative correlation between the tumour volume and perfusion index (r = 0.671, P = 0.012). The dual-input CT perfusion analysis method can be applied successfully to lung tumours. Initial results demonstrate a dual blood supply in primary lung cancer, in which the systemic circulation is dominant, and that the proportion of the two circulation systems is moderately dependent on tumour size. (orig.)

  12. Morphology of the bronchial tree of coati lungs (Nasua nasua, Linnaeus, 1966

    Directory of Open Access Journals (Sweden)

    Amilton Cesar dos Santos

    2011-06-01

    Full Text Available The coati (Nasua nasua is a diurnal, terrestrial and arboricola procionidae. It feeds extensively on the ground and uses trees to procriate, to sleep overnight and to take refuge. Its diet is omnivorous and it feeds on fruits, small vertebrates and invertebrates, nectar, eggs and vegetables. The aim of this work was to characterize microscopically the bronchial tree of the coati. Four coatis (two males and two females, fixed in formaldehyde solution 10%, were used from previous research conducted at CECRIMPAS – UNIFEOB (IBAMA-02027.003731/04-76. For this work, the tissue samples were embedded in paraffin by routine technique and processed for light microscopy in HE staining for analysis under a light microscope. It was found that microscopically, the bronchial tree of the coati is similar to that of other mammals described in the literature, i.e. it has great variations in its architecture, such as a reduced height of the epithelium which changes from ciliated pseudostratified in the larger bronchi to being cubic in the smaller bronchioles, an absence of cilia and glands in the smaller bronchioles, and a decrease in diameter and thickness of its walls.

  13. Comparison of respiratory inductance plethysmography with thoracoabdominal compression in bronchial challenges in infants and young children.

    Science.gov (United States)

    Springer, C; Godfrey, S; Vilozni, D; Bar-Yishay, E; Noviski, N; Avital, A

    1996-09-01

    Respiratory inductance plethysmography measuring thoracoabdominal asynchrony (TAA) has been claimed to be a useful tool for measuring changes in airway resistance in infants. In this study we evaluated the response to methacholine by thoracoabdominal compression and respiratory inductance plethysmography. Seventeen infants (mean age, 13.1 +/- 4.7 mo) with recurrent episodes of cough or wheeze underwent bronchial challenge with inhaled methacholine. Lung function was evaluated by measuring maximal expiratory flow at resting lung volume (VmaxFRC), and the degree of TAA was measured by phase angle (theta). Methacholine was inhaled for 1 min during tidal breathing using increasing doubling concentrations until a fall of at least 40% in VmaxFRC was achieved (final concentration). All infants responded to the final concentration of methacholine by a significant fall in VmaxFRC (from 31 +/- 10 to 12 +/- 5 ml/s/kg, p inductance plethysmography is a sensitive method to measure bronchial reactivity to methacholine in most of the infants studied (14 of 17, 82%). A concentration of methacholine causing an increase in theta of at least 2.0 standard deviations above baseline is equivalent to the concentration causing a 40% fall in VmaxFRC.

  14. Critical role of constitutive type I interferon response in bronchial epithelial cell to influenza infection.

    Directory of Open Access Journals (Sweden)

    Alan C-Y Hsu

    Full Text Available Innate antiviral responses in bronchial epithelial cells (BECs provide the first line of defense against respiratory viral infection and the effectiveness of this response is critically dependent on the type I interferons (IFNs. However the importance of the antiviral responses in BECs during influenza infection is not well understood. We profiled the innate immune response to infection with H3N2 and H5N1 virus using Calu-3 cells and primary BECs to model proximal airway cells. The susceptibility of BECs to influenza infection was not solely dependent on the sialic acid-bearing glycoprotein, and antiviral responses that occurred after viral endocytosis was more important in limiting viral replication. The early antiviral response and apoptosis correlated with the ability to limit viral replication. Both viruses reduced RIG-I associated antiviral responses and subsequent induction of IFN-β. However it was found that there was constitutive release of IFN-β by BECs and this was critical in inducing late antiviral signaling via type I IFN receptors, and was crucial in limiting viral infection. This study characterizes anti-influenza virus responses in airway epithelial cells and shows that constitutive IFN-β release plays a more important role in initiating protective late IFN-stimulated responses during human influenza infection in bronchial epithelial cells.

  15. Leukocyte peroxidase and leptin: an associated link of glycemic tolerance and bronchial asthma?

    Directory of Open Access Journals (Sweden)

    Sergio Parco

    2010-05-01

    Full Text Available Sergio ParcoImmunopathology Unit, Laboratory of the Department of Medicine, Children’s Hospital, IRCCS Burlo Garofolo, Trieste, ItalyAbstract: Recent observations suggest the presence of an interaction between leptin and the inflammatory system during bronchial asthma. Although there is evidence of a positive association between asthma and obesity in adults and children, little is yet known about the role of serum leptin, as a potential mediator for bronchial epithelial homeostasis, and intraleukocyte myeloperoxidase (MPO, a hemoprotein with a molecular weight of 140 kDa, expression of the inflammatory system, in asthmatic children. Glycemic tolerance is an important pathogenetic element in developing type 2 mellitus diabetes and a confirmed predictor of incident asthma-like symptoms in adults. This work is aimed at assessing a possible correlation between basal leukocyte myeloperoxidase levels, basal leptin and insulin-glycemic tolerance in obese children. Thirty obese children aged between 7 and 15 years were examined. The analyzed data showed a normal response to the insulinemic stimulus in children of both sexes whose basal leptin and MPO values, expressed as MPO intracellular index, werewithin the normal range.Keywords: leptin, myeloperoxidase, glycemic tolerance, asthma

  16. Dietary antioxidants and ozone-induced bronchial hyperresponsiveness in adults with asthma.

    Science.gov (United States)

    Trenga, C A; Koenig, J Q; Williams, P V

    2001-01-01

    Ozone exposure aggravates asthma, as has been demonstrated in both controlled exposures and epidemiologic studies. In the current double-blind crossover study, the authors evaluated the effects of dietary antioxidants (i.e., 400 IU vitamin E/500 mg vitamin C) on ozone-induced bronchial hyperresponsiveness in adult subjects with asthma. Seventeen subjects were exposed to 0.12 ppm of ozone or to air for 45 min during intermittent moderate exercise. Bronchial hyperresponsiveness was assessed with 10-min sulfur dioxide (i.e., 0.10 ppm and 0.25 ppm) inhalation challenges. Subjects who were given dietary antioxidants responded less severely to sulfur dioxide challenge than subjects given a placebo (i.e., forced expiratory volume in the 1st sec: -1.2% vs. 4.4%, respectively; peak flow: +2.2% vs. -3.0%, respectively; and mid-forced expiratory flow: +2.0% vs. -4.3%, respectively). Effects were more pronounced when subjects were grouped by response to sulfur dioxide at the screening visit. The results suggest that dietary supplementation with vitamins E and C benefits asthmatic adults who are exposed to air pollutants. PMID:11480500

  17. Multiple metastases to the small bowel from large cell bronchial carcinomas

    Institute of Scientific and Technical Information of China (English)

    Davor Tomas; Mario Ledinsky; Mladen Belicza; Bozo Kru(s)lin

    2005-01-01

    AIM: Metastases from lung cancer to gastrointestinal tract are not rare atpostmortem studies but the development of clinically significant symptoms from the gastrointestinal metastases is very unusual.METHODS: Formalin-fixed, paraffin-embedded tissues were cut into 5 μm thick sections and routinely stained with hematoxylin and eosin. Some slides were also stained with Alcian-PAS. Antibodies used were primary antibodies to pancytokeratin, cytokeratin 7, cytokeratin 20, epithelial membrane antigen, vimentin, smooth muscle actin and CD-117.RESULTS: We observed three patients who presented with multiple metastases from large cell bronchial carcinoma to small intestine. Two of them had abdominal symptoms (sudden onset of abdominal pain, constipation and vomiting) and in one case the tumor was incidentally found during autopsy. Microscopically, all tumors showed a same histological pattern and consisted almost exclusively of strands and sheets of poorly cohesive, polymorphic giant cells with scanty, delicate stromas. Few smaller polygonal anaplastic cells dispersed between polymorphic giant cells,were also observed. Immunohistochemistry showed positive staining of the tumor cells with cytokeratin and vimentin. Microscopically and immunohistochemically all metastases had a similar pattern to primary anaplastic carcinoma of the small intestine.CONCLUSION: In patients with small intestine tumors showing anaplastic features, especially with multiple tumors, metastases from large cell bronchial carcinoma should be first excluded, because it seems that they are more common than expected.

  18. Modified method for bronchial suture by Ramirez Gama compared to separate stitches suture: experimental study

    Directory of Open Access Journals (Sweden)

    Vitor Mayer de Moura

    2014-06-01

    Full Text Available OBJECTIVE: To experimentally compare two classic techniques described for manual suture of the bronchial stump. METHODS: We used organs of pigs, with isolated trachea and lungs, preserved by refrigeration. We dissected 30 bronchi, which were divided into three groups of ten bronchi each, of 3mm, 5mm, and 7mm, respectively. In each, we performed the suture with simple, separated, extramucosal stitches in five other bronchi, and the technique proposed by Ramirez and modified by Santos et al in the other five. Once the sutures were finished, the anastomoses were tested using compressed air ventilation, applying an endotracheal pressure of 20mmHg. RESULTS: the Ramirez Gama suture was more effective in the bronchi of 3, 5 and 7 mm, and there was no air leak even after subjecting them to a tracheal pressure of 20mmHg. The simple interrupted sutures were less effective, with extravasation in six of the 15 tested bronchi, especially in the angles of the sutures. These figures were not significant (p = 0.08. CONCLUSION: manual sutures of the bronchial stumps were more effective when the modified Ramirez Gama suture was used in the caliber bronchi arms when tested with increased endotracheal pressure.

  19. [Survey of clinical and experimental researches on mechanisms of acupuncture treatment of bronchial asthma].

    Science.gov (United States)

    Tan, Cheng; Zhao, Ji-ping; Zhang, Chang

    2011-08-01

    In the present paper, the authors review the development of experimental and clinical studies on acupuncture treatment of bronchial asthma in recent 10 years. Regarding clinical studies, results showed that acupuncture could (1) regulate cardiac-pulmonary function; and (2) adjust immune state and relieve inflammatory reactions in bronchial asthma patients. Animal experiments showed that acupuncture could function in (1) improving pulmonary function; (2) reducing accumulation of the peripheral eosinophile granulocytes (EOS), relieving the infiltration of inflammatory cells in the air-passage mucosa and promoting the apoptosis of EOS in the lung and air-passages; (3) down-regulating the expression of air-passage remodeling-related protein insulin growth factor-1; (4) suppressing the secretion of tumor necrosis factor and endothelin; (5) attenuating allergic reaction; (6) regulating neuroendocrine activity; and (7) modulating intracellular second messenger activities. However, rigorous clinical study design is not enough, so that the reliability of the results is limited. In spite of many indicators of animal experiments have been selected, but their correlations are not in close association, resulting in poor complementation and mutual identification of the acquired findings. For this reason, its clinical efficacies need to be researched further according to principles of evidence-based medicine. PMID:21942186

  20. Transfection of normal human bronchial epithelial cells with the bcl-2 oncogene

    Energy Technology Data Exchange (ETDEWEB)

    Kennedy, C.H.; Kenyon, K.D.; Tesfaigzi, J. [and others

    1995-12-01

    In vitro, studies examining the transformation of virus-immortalized human bronchial epithelial (HBE) cells after exposure to chemical and physical carcinogens have contributed to our understanding of the mechanisms that underlie the development of lung cancer. Virus-immortalized HBE cells have been used because of both the limited life span of normal human bronchial epithelial (NHBE) cells in culture (approximately 30-35 population doublins) and their resistance to in vitro malignant transformation. For example, human papillomavirus (HPV)-immortalized HBE cells have been used to study the genetic changes that occur after exposure to {alpha}-particles in vitro. Although this model may prove to be useful for studying the 18% or less of bronchogenic carcinomas found to contain HPV sequences, it is not an appropriate model for studying the majority of lung epithelial malignancies in which HPV DNA is not detected. This view is supported by the fact that HPV-immortalized cell lines commonly exhibit aneuploidy. This results of this study suggest that: (1) NHBE cells can be transiently transfected with the pCMV{Beta} vector; and (2) the antibiotic hygromycin-resistant transfected cells.