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Sample records for broiler chicks fed

  1. Weight Gain Of Broiler Chicks Fed A Maize-Soya bean Diet ...

    African Journals Online (AJOL)

    An experiment was carried out to investigate the effect of substituting synthetic methionine with sodium sulphate and sodium sulphite in a maize-soyabean diet fed to broiler chicks. Two hundred and forty (240) unsexed Hubbard day old broiler chicks were fed iso-caloric and iso-trogenous maize-soyabean ...

  2. Evaluation of growth performance of broiler chicks fed with raw and processed leucaena eucociphala seed

    International Nuclear Information System (INIS)

    Minari, J.B.; Odutuga, A.A.

    2014-01-01

    Fourty-eight broiler chicks (day-old) were used in a 4 weeks feeding experiment to assess the growth response and the performance of broiler chicks fed raw Leucaena leucociphala seed meal (RLSM), roasted L. leucociphala seed meal (RoLSM) and steamed L. leucociphala seed meal (SLSM). The L. leucociphala seed, which serve as a source of protein were subjected to two treatments (roasting and steaming). Soybean based diet served as the control. There were four (4) experimental groups, each made up of four birds in three replicates allocated to the experimental diets. The results showed that the average final live weight, average weekly weight gain and average feed intake of birds fed with RoLSM performed better than birds fed with RLSM and SLSM. It was observed that the percentage organ to body weight of birds fed with the processed LSM were significantly (P < 0.05), higher as compared to the RLSM. A significant (P < 0.05) increase in the activity of aspartate transferase in the organs (heart, kidney and liver) of broilers fed with RoLSM was observed as compared to those fed with RLSM. The results showed a significant (P < 0.05), reduction in the activity of alanine transferase of organs of broilers chicks fed with processed L. leucociphala compared to the control. It is therefore, concluded that the processing techniques applied were able to improve the nutritional quality of L. leucociphala seed meal but relatively lower to the soybean based meal. (author)

  3. Responses of broiler chicks to radiation processed full-fat rapeseed

    International Nuclear Information System (INIS)

    Farag, El-Din Diaa.M.; Abd El-Hakeim, N.F.; Ali, Y.

    1999-01-01

    Studies were undertaken to determine a safe inclusion for full-fat rapeseed processed through radiation treatment, as a step towards detoxification, in broiler chick's diet. Raw and processed full-fat seeds (10 and 20 KGy) were fed to arbor acres broiler chicks from 7 d of 49 d of age. Body weight of chicks fed the control diet were heaviest followed in order against those fed seed irradiated at 20 and 10 KGy weight depression relative to birds fed, over the experimental duration, of chicks fed diets containing raw and irradiated rapeseed at 10 and 20 KGy were-11.2, - 7.2 and - 0.14%, respectively. In general, the study indicates that processed seeds at 20 KGy fed to broilers resulted in body weights (7 wk) similar to the control birds. leg abnormalities were seen in birds fed raw and irradiated rapeseed at 10 and 20 KGy to be 31.1, 17.8 and 8.9%, respectively. Mortality rate of birds fed raw seeds was more pronounced than those fed the irradiated seeds. Birds fed raw seeds showed thyroid and liver enlargement. Processed seeds at 10 and 20 KGy reduced the effect on these organs. Feeding chicks irradiated seed at 10 and 20 KGy did not normalize the weight of thyroid and liver. Meanwhile, there is no significant difference in relative weight of gizzard, pancreas, heart and kidney of chicks fed control diet and those fed irradiated seeds

  4. GROWTH PERFORMANCE OF BROILER CHICKS FED DIETS ...

    African Journals Online (AJOL)

    Dr. A.O. Ani

    2012-06-15

    Jun 15, 2012 ... ISSN 1684–5315 ©2012 Academic Journals ... Increasing raw bambara nut waste levels also depressed (P < 0.05) nutrient absorption ... Key words: Raw bambara nut waste, enzyme, diets, broiler chicks, growth performance.

  5. Effect of phytase supplementation on apparent phosphorus digestibility and phosphorus output in broiler chicks fed low-phosphorus diets

    Directory of Open Access Journals (Sweden)

    Xian-Ren Jiang

    2015-04-01

    Full Text Available This study was conducted to evaluate the effect of supplemental phytase in broiler chicks fed different low levels of total phosphorus (P on the apparent phosphorus digestibility (APD and phosphorus output (PO in the faeces and ileal digesta. After fed a standard broiler starter diet from day 0 to 14 post-hatch, a total of 144 male broiler chicks were allocated to 6 groups for a 7-d experiment with a 2 × 3 factorial design comparing phytase (supplemented without (CTR or with 400 FTU/kg phytase (PHY and total P levels (2.0, 2.5 and 3.0 g/kg. The faecal samples were collected from day 17 to 21 post-hatch. At 22 days of age, all the chicks were slaughtered and collected the ileal digesta. Phytase supplementation significantly (P < 0.01 increased APD and decreased PO in the faeces and ileal digesta in comparison with the CTR group. In addition, PO in the faeces expressed as g/kg DM diets and faeces (Diet × P level, P = 0.047 and < 0.01, respectively as well as PO in the ileal digesta expressed as g/kg DM digesta (Diet × P level, P = 0.04 were affected by diet and P level, which were due to the significant reduction (P < 0.01 by PHY supplementation to the diets with 3.0 g/kg total P. The results evidenced that supplemental phytase improved the APD and PO when chicks was fed 3.0 g/kg total P diet, while lower total P levels may limit exogenous phytase efficacy.

  6. Performance characteristics of broiler chicks fed kidney bean as ...

    African Journals Online (AJOL)

    An experiment was conducted to investigate the effect of replacing soybean meal and groundnut cake meal with cooked and decorticated kidney bean seed meals on the performance characteristics of broilers. One hundred and eighty day old broiler chicks of Anak strain were raised on six experimental diets.

  7. Performance of Broiler Chicks Fed Irradiated Sorghum Grains

    International Nuclear Information System (INIS)

    Farag, M.D.D.; Farag, M.F. S. El-D.; Afify, A.S.

    2003-01-01

    Substitution of yellow corn with raw sorghum grains in chick diets resulted in decreases in live body weight, accumulative feed consumption and efficiency of feed utilization as compared with reference diet. Relative to raw sorghum diet, inclusion of sorghum grains irradiated at 60 and 100 kGy and/or supplemented with PEG in chick diets resulted in increases in accumulative feed consumption an efficiency feed utilization. The study suggested that irradiation treatment up to 100 kGy up grade broiler chicks performance and the combinations between radiation and PEG treatments sustain the effect of each other

  8. Haematology and serum chemistry of finisher broiler chickens fed ...

    African Journals Online (AJOL)

    This study was conducted to assess the haematology and serum chemistry of broiler chickens fed maize-cassava diets supplemented with methionine and inorganic sulphur. A total of 270 day-old broiler chicks were randomly assigned to nine treatment groups of 30 birds making 10 birds per replicate group. Starter and ...

  9. Lesions in Broiler Chicks Following Experimental Contamination ...

    African Journals Online (AJOL)

    SH

    Average daily food intake (ADFI), average daily gain (ADG), and ... that contamination of feed for broilers chicks beyond 0.5% is detrimental to the performance of broiler chicks. Introduction ... nearly all manufacturing industries. Disposal of ...

  10. Performance of broiler chicks fed on irradiated full-fat soybeans

    International Nuclear Information System (INIS)

    Diaa El-Din, M.; Farag, H.; Abd El-Hakeim, N.F.; Ali, S.Y.

    1999-01-01

    A N experiment was conducted to evaluate physiological and biochemical responses of arbor chicks from 7 to 49 days fed on diets containing raw and full-fat soybeans processed at 20 and 50 KGy. The results demonstrate that feeding chicks on raw soybeans significantly depressed growth rate and increased feed intake. A significant thyroid, liver, pancreas and gizzard enlargement were also noticed, in addition a significant reduction was found in total plasma protein and albumin during the experimental period. These responses were not as pronounced as when soybeans irradiated at 20 KGy were fad. Soybeans processed at 50 KGy markedly reduced trypsin inhibitors and haemagglutinating agent (71% and 87%, respectively) and resulted in improved chick performance and normalized thyroid, liver, pancreas and gizzard weight and total plasma protein and albumin concentrations. Plasma transaminase activities were similar in all chicks during the experimental period. The improvement in performance of groups fed processed soybeans was the correlated with reduction of trypsin inhibitors and haemagglutinating agent. It is concluded that the radiation processing of soybeans up to 50 KGy is sufficiently suitable to have potential in the feed industry without any deleterious effect on chicks' performance as evidenced by the physiological and biochemical responses

  11. Effect of Enzyme Supplementation and Irradiation of Barley on Broiler Chicks Performance

    International Nuclear Information System (INIS)

    Farag, D.H.M.; Abd El-Hakeim, N.F.

    1999-01-01

    The experiments were conducted to study the influence of irradiation treatment at dose levels of 0.20 and 60 kGy on barley beta-glucan and the effect of enzyme supplementation and irradiation of barley on broiler chicks performance. The amount of total and water-soluble beta-glucan in raw barley was 36 kg -1 , respectively. The effect of irradiation treatment on total beta-glucan was insignificant while the level of soluble beta-glucan was increased with increasing the dose levels of irradiation. The effect of irradiation treatment and enzyme supplementation of barley diets on growth and conversion performance of broiler chicks indicated that birds fed raw barley diet had lower body weight, body weight gain and feed conversion than those fed control diet throughout the experimental period. Irradiation of barley at dose of 20 kGy did not affect the chick performance (feed consumption, weight gain feed-gain ratio) that received the B 20 diet from 7 to 21 days of age, but when bird maintained on B 20 diet from 7 28 days of age, only feed-gain ratio was improved by 14.4%. The results indicate that there was a significant effect of irradiation of barley at 60 kGy (B 60) on feed -gain ratio of chicks when were fed B 60 diet from 7 to 21 days of age. The corresponding improvement in feed-gain ratio was 16.4%. When birds were fed B 60 diet from 7-28 days of age, the improvement in body weight and feed-gain ratio was 25.5 and 19.6%, respectively

  12. Influence of age of broiler chicks and duration of feeding of their ...

    African Journals Online (AJOL)

    22% CP, 3.0 Meal ME/kg) containing either raw or heat-treated full fat soyabeans were fed to 120 Anak broiler Chicks at both starter (10-45 days of age) and finisher (46-66 day of age) phase in a completely randomized design experiment to ...

  13. Lesions in Broiler Chicks Following Experimental Contamination ...

    African Journals Online (AJOL)

    The objective of the present study was to investigate the effect of feed contamination with battery waste on the performance, organs weights as well as the histology of some internal organs of broiler chicks. A total of 120 1- d old broiler chicks were allotted to four dietary treatments in a completely randomized design.

  14. Growth performance and nutrient digestibility of broiler chickens fed ...

    African Journals Online (AJOL)

    Two hundred and seventy (270), day old chicks were used in a completely randomized design experimental layout to test the growth performance and nutrient digestibility of broiler chickens fed single phase diets containing natuzyme™ treated groundnut shell at 0, 5, 10, 15, 20 and 25 % inclusion levels. The treatments ...

  15. Productive performance of broiler chicks fed rations containing irradiated meat-bone meal

    International Nuclear Information System (INIS)

    Al-Masri, M. R.

    2001-12-01

    The changes in the feed efficiency (FE) (feed intake/body weight gain), energy efficiency (EE) (metabolizable energy intake/body weight gain), total protein efficiency (TPE) (body weight gain/protein intake), metabolized energy (ME) and body weight gain and in the biological aspects of the digestive organs by broiler chicks during different periods of age (14-21 days: 3 weeks, 21-28 days: 4 weeks, 28-35 days: 5 weeks, 35-42 days: 6 weeks) were evaluated in 5 groups fed on experimental diets containing irradiated meat (0, 5, 10, 25, 50 kGy). the results showed that no significant (p 0.05) with age (comparing ages 5 and 6 weeks with 3 and 4 weeks) No significant (p<0.05) differences were noticed in FE, EE and TPE between the ages 3 and 4 weeks. There was a significant increase (p.0.05) in ME values for the age 6 weeks compared to 3, 4 and 5 weeks. No significant differences were observed in the ME values between the ages 4 and 5 weeks. (author)

  16. Nutritive value and biochemical changes in broiler chickens fed ...

    African Journals Online (AJOL)

    Castor bean (Ricinus communis Linn.) is an important oilseed rich in protein but rarely used as livestock feed due to antinutritional factors. Castor seed was detoxified using combined processing techniques of moist heating and fermentation (5 and 7- day) or lye treatment and fed to150 day-old Anak 2000 broiler chicks at ...

  17. Sensory evaluation and tibia bone retention of broiler chicken fed ...

    African Journals Online (AJOL)

    An experiment was conducted for 56 days to assess the sensory evaluation of breast meat sample and tibia bone mineralization of broiler chicken fed graded level of toasted sesame seed meal. One hundred and eighty arbor acre chicks were divided into five dietary treatments. Each treatment was replicated thrice with ...

  18. EFFECT OF DIETARY SUPPLEMENTATION OF NON-STARCH POLYSACCHARIDE DEGRADING ENZYMES ON GROWTH PERFORMANCE OF BROILER CHICKS

    Directory of Open Access Journals (Sweden)

    M. A. Nadeem, M. I. Anjum, A. G. Khan and A. Azim

    2005-10-01

    Full Text Available An experiment was conducted to study the performance and carcass parameters of broilers chicks fed diets with and without supplementing non-starch polysaccharide degrading enzymes (NSPDE at the rate of 0.5 g/kg diet. A total of 300 day-old broiler chicks were randomly divided into 12 sets (replicates each comprising 25 chicks and three sets per treatment group, reared on deep litter from 1-42 days post-hatch. Group A was fed diets without NSPDE supplementation, while group B was fed diets supplemented with NSPDE (0.5 g/kg. Group C was fed diets containing 50 kcal/kg less metabolizable energy (ME without NSPDE and group D was fed diets having 50 kcal/kg less ME with NSPDE (0.5 g/kg supplementation. Feed and water were provided ad libitum. Feed intake and feed conversion ratio (FCR from 1-28 days and 1-42 days was significantly (p<0.05 improved in chicks fed NSPDE supplemented diets (groups B and D compared to non-supplemented diets (groups A and C. However, during 29-42 days of growing period enzymes supplementation did not influence feed intake and FCR. Body weight gain, dressing percentage and relative weights of heart, gizzard and shank at 42 days of age was found to be non-significantly different among all groups. However, liver weight reduced significantly (p<0.05 in NSPDE supplemented groups. The study suggested that NSPDE supplementation was beneficial in enhancing feed utilization during the starter phase, while its effects on weight gain, dressing percentage and weights of organs, except liver weight, were found to be non-significant.

  19. Effects of Dietary Octacosanol on Growth Performance, Carcass Characteristics and Meat Quality of Broiler Chicks

    Directory of Open Access Journals (Sweden)

    L. Long

    2016-10-01

    Full Text Available Octacosanol, which has prominent physiological activities and functions, has been recognized as a potential growth promoter in animals. A total of 392 1-d-old male Arbor Acres broiler chicks with similar body weight were randomly distributed into four dietary groups of seven replicates with 14 birds each supplemented with 0, 12, 24, or 36 mg octacosanol (extracted from rice bran, purity >92%/kg feed. The feeding trial lasted for six weeks and was divided into the starter (day 1 to 21 and the grower (day 22 to 42 phases. The results showed that the feed conversion ratio (FCR was significantly improved in broilers fed a diet containing 24 mg/kg octacosanol compared with those fed the control diet in the overall phase (day 1 to 42, p = 0.042. The average daily gain and FCR both showed linear effects in response to dietary supplementation of octacosanol during the overall phase (p = 0.031 and 0.018, respectively. Broilers fed with 24 or 36 mg/kg octacosanol diet showed a higher eviscerated yield, which increased by 5.88% and 4.26% respectively, than those fed the control diet (p = 0.030. The breast muscle yield of broilers fed with 24 mg/kg octacosanol diet increased significantly by 12.15% compared with those fed the control diet (p = 0.047. Eviscerated and breast muscle yield increased linearly with the increase in dietary octacosanol supplementation (p = 0.013 and 0.021, respectively. Broilers fed with 24 or 36 mg/kg octacosanol diet had a greater (p = 0.021 pH45min value in the breast muscle, which was maintained linearly in response to dietary octacosanol supplementation (p = 0.003. There was a significant decrease (p = 0.007 in drip loss value between the octacosanol-added and the control groups. The drip loss showed linear (p = 0.004 and quadratic (p = 0.041 responses with dietary supplementation of octacosanol. These studies indicate that octacosanol is a potentially effective and safe feed additive which may improve feed efficiency and meat

  20. Use of a post-production fractionation process improves the nutritional value of wheat distillers grains with solubles for young broiler chicks

    OpenAIRE

    Thacker, Philip; Deep, Aman; Beltranena, Eduardo

    2013-01-01

    Background Post-production fractionation of wheat distillers grains with solubles (DDGS) increases their crude protein content and reduces their fiber content. This experiment was conducted to determine the effects of fractionation of wheat DDGS on apparent total tract digestibility (ATTD) and performance when fed to broiler chicks (0?21 d). Methods A total of 150, day-old, male broiler chicks (Ross-308 line; Lilydale Hatchery, Wynyard, Saskatchewan) weighing an average of 49.6 ? 0.8 g were a...

  1. Influence of Dietary Fat Source on Growth Performance Responses and Carcass Traits of Broiler Chicks

    Directory of Open Access Journals (Sweden)

    Mohammadreza Poorghasemi

    2013-05-01

    Full Text Available This study was conducted to determine the effects of three different fat sources and their combination on growth performance, carcass traits and intestinal measurements of broiler chickens reared to 42 d of age. Two hundred day-old male broiler chicks (Ross 308 were randomly assigned to one of five treatments with four replicates of 10 chicks based on a completely randomized design. The dietary treatments consisted of 4% added fat from three different sources and their combination as follows: T, diet containing 4% tallow; CO, diet containing 4% canola oil; SFO, diet containing 4% sunflower oil; TCO, diet containing 2% tallow+2% canola oil; TSFO, diet containing 2% tallow+2% sunflower oil. Dietary fat type affected significantly BW and gain as well as feed efficiency in birds fed the TCO diets compared with those fed the other diets. Dietary fat type also modified meat yield, resulting in a higher breast and drumstick yields in the birds fed TCO and TSFO diets, respectively. Most of internal organ relative weights and small intestine measurements were not influenced by dietary treatments, except for the abdominal fat pad weight that was lower in birds fed SFO and for small intestinal length that was influenced by fat source. Results from the current study suggested that the supplementation with a combination of vegetable and animal fat sources in broiler diet supported positively growth performance and carcass parameters.

  2. Water intake and digestive metabolism of broilers fed all-vegetable diets containing acidulated soybean soapstock

    Directory of Open Access Journals (Sweden)

    SL Vieira

    2006-09-01

    Full Text Available A study was conducted to compare live performance and digestive metabolism of broiler chickens fed all-vegetable diets (All-Veg compared to a regular diet including animal by-products. Three feeds were formulated and provided to broilers according to the feeding program: pre-starter from 1 to 10 days, starter from 11 to 21 days, and grower from 21 to 35 days. All feeds had corn and soybean meal as major ingredients; however, two of them were all-vegetable diets having either Degummed Soybean Oil (DSO or Acidulated Soybean Soapstock (ASS as fat sources. The third diet included poultry by-product and poultry fat. A total number of 360 day-old broiler chicks were allocated to 1m² battery cages, 10 chicks in each, and 12 replicates per treatment. Live performance was similar between groups of birds receiving the different diets with the exception of weight gain, which was increased for birds fed the All-Veg diet with ASS. Birds fed All-Veg diets had increased water intake and produced more excreta with a concurrent reduced feed metabolizability at both ages, regardless of fat source. Metabolizable Energy was not different for the three diets.

  3. Bone densitometry as an indicator of percentage tibia ash in broiler chicks fed varying dietary calcium and phosphorus levels.

    Science.gov (United States)

    Onyango, E M; Hester, P Y; Stroshine, R; Adeola, O

    2003-11-01

    The relative sensitivity of tibia bone mineral content (BMC) and density (BMD), percentage ash (ash), and shear force as indicators for dietary Ca and P was compared in 3-wk-old broiler chicks. One hundred eight 7-d-old chicks were grouped by weight into 6 blocks of 3 cages each with 6 birds per cage. Three corn-soybean meal-based diets were randomly assigned to cages within each block. The diets were low P, medium P, and adequate P and were formulated to contain 4.0, 5.1, and 7.8 g of total P/kg feed, respectively; and 5.1, 6.7, and 10.0 g of Ca/kg feed, respectively. The chicks were fed the experimental diets for 14 d. On d 22, chicks were killed, and tibiae were removed from 3 birds/cage. Weight gain, feed intake, feed efficiency, BMC, BMD, shear force, and ash were determined. The BMC and BMD were determined using dual energy x-ray absorptiometry. Correlations among the various bone status variables and dietary Ca and P were determined. Growth performance criteria and ash increased linearly, and BMC and BMD increased linearly and quadratically as dietary concentrations of Ca and P increased. The correlation coefficient between dietary Ca and BMC, BMD, shear force, or ash was 0.89, 0.91, 0.50, or 0.89, respectively; and between dietary P and BMC, BMD, shear force, or ash was 0.88, 0.91, 0.48, or 0.89, respectively. The correlation coefficient between ash and BMC, BMD, or shear force was 0.92, 0.93, or 0.67, respectively. The correlation coefficients for linear regression between shear force and BMC or BMD was 0.56. The regression model for predicting percentage ash using BMD was as follows: percentage ash = 24 + (240 x BMD) with an r2 of 86%. It is concluded that in broiler chicks, tibia ash, BMC, and BMD may be more sensitive than shear force as indicators of dietary Ca and P concentrations and that BMD as measured by dual energy x-ray absorptiometry may be used to predict percentage of tibia ash.

  4. Insoluble granite-grit allows broiler chicks to have better growth performance and gut health

    Directory of Open Access Journals (Sweden)

    Güray Erener

    Full Text Available ABSTRACT The objective of this study was to investigate the effects of insoluble granite-grit on growth performance and gut health status of broiler chicks. One hundred and sixty 14-day-old broiler chicks were allocated to experimental groups control and grit feeding. Each group included 80 birds divided by four replicates, each consisting of 20 birds, 10 males and 10 females. Feed intake, body weight gain, feed conversion ratio, carcass weight, dressing percentage, gut length, abdominal fat pad, and liver, heart, and gizzard weights were determined. Starter diet included 230 g crude protein (CP kg−1 and 3.15 Mcal metabolizable energy (ME kg−1 during the days 14 to 21; grower diet included 212 g CP kg−1 and 3.20 Mcal ME kg−1; and finisher diet included 190 g CP kg−1 and 3.20 Mcal ME kg-1. Control birds were fed broiler diets, while grit-fed birds were allowed to consume broiler diets, including 3% insoluble granite-grit. Grit feeding improved the final body weight (2268 vs. 2336 g with better feed efficiency (2.03 vs. 1.95 and less feed intake (3780 vs. 3764 g. Grit feeding increased gizzard weight (31.63 vs. 48.00 g without affecting carcass weight (1643 vs. 1708 g, dressing percentage (73.50 vs. 73.60%, liver weight (42.69 vs. 40.69 g, heart weight (12.00 vs. 11.63 g, abdominal fat pad (34.50 vs. 35.38 g, and gut length (229.3 vs. 220.7 cm of broilers. Grit feeding affects daily gain, feed intake, and feed conversion ratio positively by increasing gizzard development and health.

  5. Genotoxic effects of deoxynivalenol in broiler chickens fed low-protein feeds.

    Science.gov (United States)

    Awad, W A; Ghareeb, K; Dadak, A; Gille, L; Staniek, K; Hess, M; Böhm, J

    2012-03-01

    Deoxynivalenol (DON) is one of the most abundant and important trichothecenes in food and feed, and it is a significant contaminant due to its frequent occurrence at toxicologically relevant concentrations worldwide. Deoxynivalenol has negative influences on the health and performance of chicks. However, there is little information available regarding the effect of DON on DNA fragmentation in blood lymphocytes. In addition, the effects of Mycofix select (Biomin GmbH, Herzogenburg, Austria) supplementation to DON-contaminated broiler diets on lymphocyte DNA have not yet been demonstrated. Therefore, the aim of the present study was to establish the effect of DON on lipid peroxidation and lymphocyte DNA fragmentation in broilers and to evaluate the potential of Mycofix select in the prevention of toxin-mediated changes. Thirty-two 1-d-old (Ross 308 male) broiler chicks were randomly divided into 4 groups. The control group was fed a noncontaminated diet, and a second group was fed the same diet but supplemented with Mycofix select (0.25%). A third group of broilers was fed a diet artificially contaminated with 10 mg of feed-grade DON/kg of diet, and a fourth group was fed a DON-contaminated diet supplemented with Mycofix select. At the end of the feeding trial, blood was collected and the degree of lymphocyte DNA damage was measured in the plasma by comet assay. Deoxynivalenol increased (P = 0.016) the amount of DNA damage in chicken lymphocytes by 46.8%. Mycofix select protected lymphocyte DNA from the DON effects. To our knowledge, these are the first data on genotoxic effects of a moderate dose of DON on chicken lymphocytes. However, the thiobarbituric acid reactive substances level in liver and liver enzyme activity did not differ among the groups. In conclusion, the present study demonstrated that the diets contaminated with the mycotoxin DON at moderate levels in combination with low-protein feed are able to induce lymphocyte DNA damage in chickens

  6. Perinatal broiler physiology between hatching and chick collection in 2 hatching systems

    NARCIS (Netherlands)

    Ven, van de L.J.F.; Wagenberg, van A.V.; Decuypere, E.; Kemp, B.; Brand, van den H.

    2013-01-01

    Little is known about physiological responses of early- versus late-hatching chicks to early posthatch conditions in broiler practice. We investigated effects of hatching time on perinatal broiler physiology in 2 hatching systems, differing in conditions: a conventional hatcher, where chicks are

  7. Estimation of Abdominal Fat by Plasma Triglycerides and Carcass Dry Matter in Broiler Chicks

    Directory of Open Access Journals (Sweden)

    Javad Pour-Reza

    1997-04-01

    Full Text Available This experiment was carried out to determine the relationship of plasma triglycerides and carcass dry matter with abdominal fat. One of the problems of broiler is carcass fatness, especially abdominal fat due to rapid growth of broilers which is not the consumer preference. Six hundred one-day-old commercial broiler chicks (Lohman were divided into 40 groups, 15 chicks per group. Each one of the 10 experimental diets, in which barley was substituted for corn at levels of 0, 5, 10, 20 and 40%, was fed to 4 groups of chicks for 56 days. All diets were isoenergetic and isonitrogenous. Rice hulls was used to make diets isoenergetic. At days 42, 49 and 56, one male and one female were selected from each pen and after blood sampling, the birds were killed, eviscerated and percentages of carcass and amount of abdominal fats were measured. Blood samples were used to determine plasma triglycerides, cholesterol and total lipids. The results showed that increasing abdominal fat reduced carcass moisture content. Correlation between abdominal fat and plasma triglycerides was positive and significant (p<0.05. Correlation between abdominal fat and carcass moisture was negative and significant (P<0.01. The regression equations indicated that abdominal fat can be estimated from plasma triglycerides and carcass moisture content. The equations also indicated that using several parameters for estimating abdominal fat is better than single parameter estimation.

  8. Growth performance of broilers fed on sprouted-roasted guar bean (Cyamopsis tetragonoloba) based diets.

    Science.gov (United States)

    Madzimure, James; Muchapa, Lorraine; Gwiriri, Lovemore; Bakare, Archibold G; Masaka, Lawrence

    2017-06-01

    In a completely randomized block design with 96 Cobb-500 broilers, a study was conducted to evaluate the potential of dietary inclusion of sprouted then roasted guar bean in broiler diets. The 96 male day-old broiler chicks, blocked by pen into equal weight groups of six chicks replicated four times per treatment, were randomly allocated to treatment diets containing graded levels of sprouted then roasted guar bean meal (GBM) at 0, 50, 100 and 150 g kg -1 inclusion level. The guar bean was sprouted and roasted to reduce guar gum effect. Total feed intake decreased significantly as the guar bean meal content increased in the starter phase (P  0.05) were observed. Diets containing 0 and 50 g kg -1 GBM recorded significantly higher total feed intake compared to the diet containing 150 g kg -1 GBM. Although average weight gain was not significantly different in birds fed 0 and 50 g kg -1 GBM diets, it was significantly higher than in birds fed on 100 and 150 g kg -1 GBM diets. Feed conversion ratio was not significantly different among treatment groups (P > 0.05) but showed a general decreasing trend with increasing guar bean meal inclusion level, the effect being more pronounced during the starter phase. In conclusion, the optimum inclusion level of sprouted then roasted guar bean meal in broiler diets is 50 g kg -1 .

  9. Ameliorative effect of a microbial feed additive on infectious bronchitis virus antibody titer and stress index in broiler chicks fed deoxynivalenol.

    Science.gov (United States)

    Ghareeb, K; Awad, W A; Böhm, J

    2012-04-01

    Although acute mycotoxicoses are rare in poultry production, chronic exposure to low levels of mycotoxins is responsible for reduced productivity and increased susceptibility to infectious diseases. Deoxynivalenol (DON) is known to modulate immune function, but only a few studies have investigated the effect of DON on the vaccinal immune response. In addition, the effects of Mycofix select (Biomin GmbH, Herzogenburg, Austria) supplementation to DON-contaminated broiler diets have not yet been demonstrated. Therefore, an experiment with 1-d-old male broilers (Ross 308) was carried out to examine the effects of feeding DON-contaminated low-protein grower diets on performance, serum biochemical parameters, lymphoid organ weight, and antibody titers to infectious bronchitis vaccination in serum and to evaluate the effects of Mycofix select dietary supplementation in either the presence or absence of DON in broilers. In total, thirty-two 1-d-old broiler chicks were randomly assigned to 1 of the 4 dietary treatments for 5 wk. The dietary treatments were 1) control; 2) artificially contaminated diets with 10 mg of DON/kg of diet; 3) DON-contaminated diets supplemented with Mycofix select; and 4) control diet supplemented with Mycofix select. Feeding of contaminated diets decreased (P = 0.000) the feed intake, BW (P = 0.001), BW gain (P = 0.044), and feed efficiency during the grower phase. Deoxynivalenol affected the blood biochemistry, whereas plasma total protein and uric acid concentrations in birds fed contaminated grains were decreased compared with those of the controls. Moreover, in birds fed contaminated feeds, there was a tendency to reduce triglycerides in the plasma (P = 0.090), suggesting that DON in the diets affected protein and lipid metabolism in broiler chickens. The feeding of contaminated diets altered the immune response in broilers by reducing the total lymphocyte count. Similarly, the antibody response against infectious bronchitis vaccination

  10. Determination of endogenous manganese excretion in broiler chicks by an isotope-dilution method

    International Nuclear Information System (INIS)

    Weigand, E.; Kirchgessner, M.; Kilic, A.

    1988-01-01

    A tracer study with broiler chicks was conducted to determine endogenous Mn excretion in response to graded levels of dietary Mn supply by the isotope-dilution method. Starting with 46 day-old chicks, low-Mn diets were fed for 12 days before 8 chicks were slaughtered for assessing the intial Mn status and 38 chicks each were given an intramuscular injection of 555 kBq 54 Mn. Four groups of six birds each were fed, one bird per cage, diets containing 2.8, 9.1, 25.8, and 59.7 ppm Mn for 16 days before slaughter and carcass analyses. Two groups of 7 birds were fed the diets with 9.1 and 25.8 ppm Mn for 8 days. Mn supply significantly affected 54 Mn excretion activity of distribution among various body organs, especially the liver. The specific activity of Mn in excreta and in whole-body declined exponentially with time and differed between groups. Endogenous Mn in excreta was estimated on the basis that the specific activity of excreta Mn from day 9 to 12 and day 13 to 16 was reflected, on the average, by that of liver Mn on day 10 and 14, respectively. It averaged in the order of increasing Mn supply 12.5 vs. 12.0; 7.7 vs. 8.5; 3.9 vs. 4.3, and 2.0 vs. 2.3% of total excreta Mn. Total Mn excretion from day 9 to 16 averaged 88.9, 95.0, 97.9. and 98.9% of Mn intake. (author)

  11. CARCASS YIELD OF BROILER CHICKENS FED BANANA (Musa paradisiaca LEAVES FERMENTED WITH Trichoderma viride

    Directory of Open Access Journals (Sweden)

    J. S. Mandey

    2016-03-01

    Full Text Available The study was conducted to evaluate the effect of level of banana (Musa paradisiaca leaves fermented with Trichoderma viride at different days on the carcass yield of broiler chickens. A hundred and eighty 3-weeks-old broiler chicks were used in this present experiment based on factorial design (3×4. The birds were randomly allocated into three experimental diets containing of 5, 10 and 15% of banana leaves fermented within 0, 5, 10 and 15 days. Each treatment was divided into three replicates of five chicks in each. The experiment was terminated after 4 weeks or when the birds were 7-weeks-old. Feed intake, body weight gain, feed efficiency and carcass yield were measured during the study. The data were subjected to the analysis of variance test followed by least significant difference (LSD test. Results showed that daily feed intake was significantly affected (P˂0.01 by the dietary treatments, in which feed intake was highest in broilers fed diet containing 10% banana leaves fermented for 10 days. The daily weight gain, feed efficiency and carcass yield were significantly affected (P˂0.01 by the treatments, in which the highest values of daily weight gain, feed efficiency, and carcass yield were observed in birds fed diet containing 10% banana leaves fermented for 10 days. It can be concluded that diet containing 10% banana leaves fermented for 10 days can be included in broiler ration without detrimental effects on the performance and carcass yield. 

  12. AFCF and clinoptilolite use in reduction of (137)Cs deposition in several days' contaminated broiler chicks.

    Science.gov (United States)

    Mitrovic, B; Vitorovic, G; Vitorovic, D; Dakovic, A; Stojanovic, M

    2007-01-01

    The objective of this study was to investigate the binding efficiency of AFCF and clinoptilolite, mixed to the feed and administered orally using gastric tube to chronically (137)Cs alimentary contaminated broiler chicks. Seventy-five male Hybro broiler chicks, between 35 and 47 days of age were divided into five groups (15 birds per group) reared in cages (five birds in a cage) and fed a standard diet. Every day during 13 days of the experimental period all chicks received orally 1 ml CsCl water solution with activity of 1310 Bq ml(-1)(137)Cs (gastric tube). Group 1 was the control group and received no binders. The experimental groups received the binders. Group 2 received 0.2 g of AFCF in the form of water solution (gastric tube); group 3 received 0.2% AFCF in the feed; group 4 received 2g clinoptilolite in the form of water suspension (gastric tube) and group 5 received 2% clinoptilolite in the feed. Five chicks from each group were sacrificed on days 4, 10 and 13 of the experimental period. Using gamma spectrometric methods specific activity of (137)Cs was determined in the samples of breast meat, liver and gizzard. The results obtained showed that administering binders to the chronically contaminated broiler chicks significantly (pmeat, liver and gizzard. Decreasing deposition of (137)Cs in breast meat and internal organs increased with time of contamination and binders' administration. With AFCF as a cesium binder, on day 13 of measuring the (137)Cs activity in breast meat was 80-83% lower than that in the control group, 89% in liver and 83-84% in gizzard. Natural clinoptilolite demonstrated lower binding efficiency. On day 13 of measuring the (137)Cs activity in breast meat was 53-69% lower than that in the control group, 67-60% in liver and 59-71% in gizzard.

  13. Effect of Pigeon pea and Cow pea on the performance and gut immunity of broiler chicks

    International Nuclear Information System (INIS)

    Yagoub, Yagoub Magboul

    1998-03-01

    two experiments were conducted to examine the effect of pigeon pea and cow pea on the performance and gut immunity of broiler chicks. In experiment 1, 3 experimental diets were formulated containing graded levels of cow pea were maintained. Diets were prepared containing 18.21, 18.25 and 18.25% crude protein and 3076.41, 3062 Kel/Kg metabolizable energy for experiment 1, while diets of experiment 11 were prepared containing 18.21, 18.22, and 18.22% crude protein and 3076.41, 3080.5 and 3055.89 KEl/Kg metabolized energy. 120 Loghmann broiler chicks were equally allocated into 15 pens (8 chicks/pen). Then the experimental diets were randomly assigned to the pens. feed and water were provided ad libitum in both experiments. In experiment 1, the results showed no significant difference were found in chick performance at day 45. The feed conversation ratio increased with the level of pigeon pea used. The pancreas mass was increased as the level of pigeon pea increase. In experiment 2 the results showed significant decrease in the body weight and feed intake at day 45, while the pancreas mass tend to increase with increasing level of cow pea in the diet. Histological examination of small intestine slides showed no histopathological differences between the control and chicks fed cow pea and/or pigeon pea. Immunological test of the serum and mucous samples using ELISA techniques revealed no significant difference between the control and chicks given cow pea and / or pigeon pea

  14. Performance of broiler chicken fed multicarbohydrases supplemented low energy diet

    Directory of Open Access Journals (Sweden)

    Kumar Govil

    2017-07-01

    Full Text Available Aim: Objective of this study was to investigate the effect of multicarbohydrases supplementation on performance of broilers fed low energy diet. Materials and Methods: A total of 75 days old chicks were selected and randomly divided into three treatments groups (T1, T2, and T3; each group contained 25 chicks distributed in five replicates of five chicks each. T1 group (positive control was offered control ration formulated as per Bureau of Indian Standards recommendations. In T2 group (negative control ration, metabolizable energy (ME was reduced by 100 kcal/kg diet. T3 group ration was same as that of T2 except that it was supplemented with multicarbohydrases (xylanase at 50 g/ton+mannanase at 50 g/ton+amylase at 40 g/ton. Feed intake and body weight of all experimental birds were recorded weekly. Metabolic trial was conducted for 3 days at the end of experiment to know the retention of nutrients. Results: Significant improvement (p<0.01 was observed in total weight gain, feed conversion efficiency, and performance index in broilers under supplementary group T3 as compared to T1 and T2 groups. Retention of crude protein and ether extract was significantly increased (p<0.05 in T3 group supplemented with multicarbohydrases as compared to other groups. Retention of dry matter, crude fiber, and nitrogen-free extract was comparable in all the three groups. Significantly highest dressed weight, eviscerated weight, and drawn weight (% of live body weight were observed in multicarbohydrases supplemented T3 group, however it was comparable in T1 and T2 groups. Conclusion: It was concluded that the supplementation of multicarbohydrases (xylanase at 50 g/ton+mannanase at 50 g/ton+amylase at 40 g/ton in low energy diet improved overall performance of broilers.

  15. Physiological responses of newly hatched broiler chicks to increasing journey distance during road transportation

    Directory of Open Access Journals (Sweden)

    Heshmatollah Khosravinia

    2015-09-01

    Full Text Available This study was conducted to examine the effects on weight loss (WL, residual yolk (RY utilization and serum biochemical parameters of transport distance (TD in newly hatched broiler chicks. Just after hatching, 200 Ross 308 broiler chicks were traveled over 1000 km. At hatchery and after 200, 400, 600, 800 and 1000 km journey, a random sample of 20 birds were killed for blood collection, WL and yolk weight assessment. Live weight decreased linearly by 0.42 and 0.48 g per 100 km of journey in males and female chicks, respectively. Yolk sac residual utilized by 0.071 and 0.069 g per 100 km of travel in males and females chicks, respectively, over journey distances up to 800 km. Extended journey from 800 to 1000 km caused a great decline of 0.51 and 0.58 g per 100 km in yolk weight in male and female chicks, respectively. Serum Ca concentration reduced in TDs up to 400 km, then increased for longer journeys (P<0.05. Over 1000 km journey, serum GLU level of 196.24 mg/dL at hatchery was decreased to 117.31 mg/dL at the end of journey. Serum urea, uric acid and cholesterol concentrations were increased with the extended TDs (P<0.05. In conclusion increasing transportation distance causes intensified physiological stress in broiler chicks with varying effect on different stress indicators. Stress indicators in newly hatched chicks in transit may differ from those proposed for replaced chicks and the broiler chicken at later ages.

  16. Production traits of broiler chicken strains fed Ad Libitum and raised ...

    African Journals Online (AJOL)

    ... of commercial broiler chickens reared on full-feeding, and under the same housing, feeding regime, agro-climatic zone and management practices. A total number of 150 broiler dayold chicks, that, is 50 chicks each of Arbor Acres (strain A), Hubbard (strain B) and Marshall (strain C) were purchased from local hatcheries, ...

  17. In Ovo and dietary administration of oligosaccharides extracted from palm kernel cake influence general health of pre- and neonatal broiler chicks.

    Science.gov (United States)

    Faseleh Jahromi, Mohammad; Shokryazdan, Parisa; Idrus, Zulkifli; Ebrahimi, Rohollah; Liang, Juan Boo

    2017-01-01

    Palm kernel cake (PKC) is the main byproduct from the palm oil industry in several tropical countries that contains considerable amounts of oligosaccharide. We earlier demonstrated beneficial prebiotic effects of oligosaccharides extract of PKC (OligoPKC) in starter and finisher broiler birds. This study was envisaged to elucidate the effects of in ovo and/or oral administration of the OligoPKC on prenatal and post-hatched broiler chicks. A total of 140 broiler (Cobb500) eggs were randomly divided into two groups (n = 70 each), and on day 12 of incubation, eggs in one group received in ovo injection of 0.1 mL (containing 20 mg) of OligoPKC, while those in the other group received 0.1 mL of saline (placebo) solution. Of these in ovo placebo or OligoPKC injected eggs, after hatching, six chicks from each group were sampled for day-one analysis, while 48 chicks from each group were randomly allocated to two dietary regimes involving either no feeding or feeding of OligoPKC through basal diet for a 14 days experiment forming the experimental groups as: (i) saline-injected (Control, C), (ii) OligoPKC-injected (PREBovo), (iii) saline-injected, but fed 1% OligoPKC (PREBd), and (iv) OligoPKC-injected and also 1% OligoPKC (PREBovo+d). In ovo injection of prebiotic OligoPKC had no effect on body weight and serum immunoglobulins concentrations of day old chicks, except for IgG, which was increased significantly (P C and PREBovo), but lesser influenced by in ovo OligoPKC injection. Irrespective of its prior in ovo exposure, chicks fed OligoPKC diets had lower population of pathogenic bacteria. Overall serum immunoglobulin status of birds was improved by feeding of OligoPKC but in ovo OligoPKC injection had minor effect on that. In most cases, in ovo OligoPKC injection and feeding of OligoPKC reduced the expression of nutrient transporters in the intestine and improved antioxidant capacity of liver and serum. It is concluded that in ovo injection of OligoPKC increased Ig

  18. Effect of Milk Thistle (Silybum marianum L. on Biochemical Parameters and Immunity of Broiler Chicks Fed Aflatoxin B1 after Three Weeks

    Directory of Open Access Journals (Sweden)

    Maliheh Amiri Dumari

    2014-09-01

    Full Text Available Background: This study was conducted to determine the efficacy of milk thistle seeds (MTSs in counteracting the toxic effects of aflatoxin B1 (AFB1 in a contaminated diet fed to broilers. Methods: Two dietary inclusion rates of AFB1 (0, 0.250 and 500 ppb and MTS (0, 0.5 and 1% were tested in a 3×3 factorial manner. The effect of nine experimental treatments was assessed using 216 one-d-old Ross 308 male broiler chicks in a randomized complete design with 4 replicates of 6 birds each from one to 21 days of age. The effects of dietary AFB1 and MTS on serum biochemistry factors, antibody titer against Newcastle disease (ND and influenza disease (ID in broilers were evaluated at the end of this period. Results: Statistical analysis of the main effects of diets indicated no significant changes in uric acid, cholesterol, triglycerides, low density lipoprotein (LDL, ID, and phosphorus compared to the control (P>0.01. Also, addition of 500 ppb of dietary AFB1 into the diet was associated with significant decreases in serum glucose, calcium, high density lipoprotein (HDL, and ND compared to the control group (P<0.01. The contaminated diet significantly increased the activities of aspartate aminotransferase (AST and alanine aminotransferase (ALT (P<0.05. Conclusion: Milk thistle showed protective effects and resulted in some serum enzyme activities and serum biochemical changes associated with aflatoxin toxicity.

  19. AFCF and clinoptilolite use in reduction of 137Cs deposition in several days' contaminated broiler chicks

    International Nuclear Information System (INIS)

    Mitrovic, B.; Vitorovic, G.; Vitorovic, D.; Dakovic, A.; Stojanovic, M.

    2007-01-01

    The objective of this study was to investigate the binding efficiency of AFCF and clinoptilolite, mixed to the feed and administered orally using gastric tube to chronically 137 Cs alimentary contaminated broiler chicks. Seventy-five male Hybro broiler chicks, between 35 and 47 days of age were divided into five groups (15 birds per group) reared in cages (five birds in a cage) and fed a standard diet. Every day during 13 days of the experimental period all chicks received orally 1 ml CsCl water solution with activity of 1310 Bq ml -1 137 Cs (gastric tube). Group 1 was the control group and received no binders. The experimental groups received the binders. Group 2 received 0.2 g of AFCF in the form of water solution (gastric tube); group 3 received 0.2% AFCF in the feed; group 4 received 2 g clinoptilolite in the form of water suspension (gastric tube) and group 5 received 2% clinoptilolite in the feed. Five chicks from each group were sacrificed on days 4, 10 and 13 of the experimental period. Using gamma spectrometric methods specific activity of 137 Cs was determined in the samples of breast meat, liver and gizzard. The results obtained showed that administering binders to the chronically contaminated broiler chicks significantly (p 137 Cs transfer and deposition in breast meat, liver and gizzard. Decreasing deposition of 137 Cs in breast meat and internal organs increased with time of contamination and binders' administration. With AFCF as a cesium binder, on day 13 of measuring the 137 Cs activity in breast meat was 80-83% lower than that in the control group, 89% in liver and 83-84% in gizzard. Natural clinoptilolite demonstrated lower binding efficiency. On day 13 of measuring the 137 Cs activity in breast meat was 53-69% lower than that in the control group, 67-60% in liver and 59-71% in gizzard

  20. Efficiency of Tribulus terrestris L. as an antibiotic growth promoter substitute on performance and immune responses in broiler chicks

    Directory of Open Access Journals (Sweden)

    Faramarz Fekri Yazdi

    2014-09-01

    Full Text Available Objective: To evaluate the effect of Tribulus terrestris L. (puncture vine as an antibiotic growth promoter substitute on growth performance, carcass traits, and immune responses in broiler chickens. Methods: A total of 1 92 one-day-old as hatched broiler chicks (Ross 308 were randomly allocated to four treatment groups, with 4 replicates of 12 birds. The following treatments were applied: basal diet (control, control+4.5 mg flavophospholipol/kg, or control+1 or 5 g puncture vine powder/kg. Vaccines against newcastle, influenza disease, and sheep red blood cells were administered to immunological stimuli. Results: Daily feed intake, internal organ weights, and carcass traits were not influenced by the dietary treatments except for carcass yield that increased in broilers fed diet supplemented with 1 g puncture vine powder/kg at 42nd day. Broilers receiving 1 g puncture vine/kg tended to have a lower feed conversion ratio compared to other groups during starter, finisher and entire experimental period (P>0.05. Broilers receiving 1 or 5 g puncture vine/kg had higher antibody titer against Newcastle disease virus compared to other groups (P<0.05. Broilers receiving 1 g puncture vine/kg had the highest antibody titer against avian influenza virus and sheep red blood cells at 28 and 31 days of age, respectively (P<0.05. Conclusions: In conclusion, the overall results of the current study showed that puncture vine powder seems to have the potential to positively influence growth performance and immune responses of broiler chicks.

  1. Comparative study on the effect of boiling, autoclaving and irradiation treatments of artichoke by-product on growth perfor mance and digestibility of broiler chicks

    International Nuclear Information System (INIS)

    Mekkawy, S.H.; Zakaria, S.M.; El-Faramawy, A.A.

    2003-01-01

    Sixty broiler chicks (21 days old) were assigned into six equal groups. artichoke by-product (ABP) was subjected to different treatments (Boiling, autoclaving and gamma irradiation at 75 and 100 kGy). The objective of the present study was to evaluate the effects of using ABP with different treatments on growth performance and their nutrient digestibility. Results revealed that, chicks fed diets containing raw ABP or irradiated with 100 kGy showed the highest values of live body weight, body weight gain, feed intake and dressing percentages compared with those fed a control diet. The lowest values of those parameters resulted by the chicks fed the diets of boiling or autoclaving ABP. Digestibility coefficients of crude protein, nitrogen free extract, crude fiber and organic matter increased by increased by increasing dose level of gamma irradiation. It could be recommended to use ABP in raw state up to 10% replacement of yellow corn without any adverse effects on chick performance. The use of irradiation treatment of ABP at 100 kGy improved remarkable feed utilization and growth performance

  2. Late metabolic changes in broiler chicks after irradiation of setting eggs

    International Nuclear Information System (INIS)

    Falis, M.; Striskova, K.; Dvorak, P.

    2006-01-01

    In our experiment setting eggs of broiler chicks were exposed to single dose of gamma radiation (0.25 Gy) before putting into hatcher. Analyses were performed in chicks, 51-day old, after hatching. Statistically significant decrease in glucose concentration and ALT activity and increase in serum total proteins in chicks that were exposed to irradiation during embryonal stage of development were recorded. Irradiation of chicks in this stage with low doses of ionising radiation may significantly influence the metabolism of individuals in subsequent developmental periods. (authors)

  3. Performance and ileal characteristics of finishing broilers fed diets ...

    African Journals Online (AJOL)

    An experiment was conducted to evaluate the effect of prebiotics supplemented diets on performance characteristics and gut morphology of broiler chickens. The study involved 320 day-old Anak broiler chicks, used to assess the utilization of prebiotics [Mannose oligosaccharides (MOS) and Lactose oligosaccharides ...

  4. Effects of multi-carbohydrase and phytase on standardized ileal digestibility of amino acids and apparent metabolizable energy in canola meal fed to broiler chicks.

    Science.gov (United States)

    Gallardo, C; Dadalt, J C; Kiarie, E; Trindade Neto, M A

    2017-09-01

    Two assays were conducted to evaluate nutritive value of canola meal (CM) fed to broiler chicks without or with a multi-carbohydrase (MC) preparation (700 U α-galactosidase, 2,200 U galactomannanase, 30,000 U xylanase, and 22,000 U β-glucanase per kg of diet) and phytase (Phy, 500 FTU per kg of diet). Assay 1 determined apparent total tract digestibility (ATTD) of nutrients and metabolizable energy (AME) by the difference method. Assay 2 determined apparent ileal digestibility (AID) and standardized ileal digestibility (SID) of amino acids by the index method. Two reference diets (RD) - an 85% corn based and a 5% casein-cornstarch diet fortified with vitamins and minerals - were made for assays 1 and 2, respectively. For each assay, the test diets were made by mixing RD and CM 7:3 wt/wt basis and fed without or with MC or Phy or combination. A total of 245 day-old male broilers (Cobb 500) was allocated to 5 treatments to give 7 replicates (7 birds/cage). The birds were fed a commercial diet from day zero to 10 followed by assay 1 fed from d 11 to 18 and assay 2 fed from d 19 to 21. Excreta samples were collected on d 15 to18, and all birds were slaughtered on d 21 for ileal digesta. There was an interaction (P  0.05) between MC and Phy on AMEn; however, MC and Phy individually improved AMEn retention. Enzymes interacted (P < 0.05) on SID of Arg, His, Leu, Met, Thr, Ala, Asp, Gln, and Gly. In this context, feeding a combination of MC and Phy resulted in higher (P < 0.05) SID of Arg, His, Met, and Thr relative to single activity or control. Both enzymes improved (P < 0.05) SID of Lys independently. The combination of carbohydrase and Phy may be an effective strategy to improve amino acid utilization in CM for poultry. © 2017 Poultry Science Association Inc.

  5. Effects of Different Levels of Coriander (Coriandrum sativum Seed Powder and Extract on Serum Biochemical Parameters, Microbiota, and Immunity in Broiler Chicks

    Directory of Open Access Journals (Sweden)

    Hesam Hosseinzadeh

    2014-01-01

    Full Text Available The use of herbs and spices has gained increasing interest as feed additives and possible alternative to antibiotics in poultry production. The effects of using different levels of coriander seed powder or extract on selected blood parameters, intestinal microflora, and immune response of broiler chickens were investigated in this study. A total of 420-day-old broiler chicks were randomly assigned to 7 treatments with 4 replicates and fed for 42 days. Results showed that inclusion of 2.0% coriander powder in broiler diets lowered total cholesterol while blood urea was significantly higher in birds on T4 compared to T1 and T2. Furthermore, there were no treatment effects on Lactobacillus bacteria; however, the population of E. coli was significantly higher in the ileum of chickens fed T0. Noticeable significant improvements of antibody titer against Newcastle, infectious bronchitis, and infectious bursal disease were observed in birds receiving coriander extract in water. Immunoglobulin G antibody against sheep red blood cells showed significant improvement in birds fed T3; likewise, immunoglobulin M was significantly higher in birds on T2 and T3 at 28 d of age. These results revealed that coriander extract or powder can be used as antibiotic alternative in broiler feeds.

  6. AFCF and clinoptilolite use in reduction of {sup 137}Cs deposition in several days' contaminated broiler chicks

    Energy Technology Data Exchange (ETDEWEB)

    Mitrovic, B. [Faculty of Veterinary Medicine, Department of Radiology, 18 Bulevar JA, 11 000 Belgrade (Serbia)]. E-mail: radijacija@vet.bg.ac.yu; Vitorovic, G. [Faculty of Veterinary Medicine, Department of Radiology, 18 Bulevar JA, 11 000 Belgrade (Serbia); Vitorovic, D. [Faculty of Agriculture, Department of Animal Science, 6 Nemanjina, 11 080 Belgrade (Serbia); Dakovic, A. [Institute for Technology of Nuclear and Other Raw Materials, 86 Franche D' Epere, 11 000 Belgrade (Serbia); Stojanovic, M. [Institute for Technology of Nuclear and Other Raw Materials, 86 Franche D' Epere, 11 000 Belgrade (Serbia)

    2007-06-15

    The objective of this study was to investigate the binding efficiency of AFCF and clinoptilolite, mixed to the feed and administered orally using gastric tube to chronically {sup 137}Cs alimentary contaminated broiler chicks. Seventy-five male Hybro broiler chicks, between 35 and 47 days of age were divided into five groups (15 birds per group) reared in cages (five birds in a cage) and fed a standard diet. Every day during 13 days of the experimental period all chicks received orally 1 ml CsCl water solution with activity of 1310 Bq ml{sup -1} {sup 137}Cs (gastric tube). Group 1 was the control group and received no binders. The experimental groups received the binders. Group 2 received 0.2 g of AFCF in the form of water solution (gastric tube); group 3 received 0.2% AFCF in the feed; group 4 received 2 g clinoptilolite in the form of water suspension (gastric tube) and group 5 received 2% clinoptilolite in the feed. Five chicks from each group were sacrificed on days 4, 10 and 13 of the experimental period. Using gamma spectrometric methods specific activity of {sup 137}Cs was determined in the samples of breast meat, liver and gizzard. The results obtained showed that administering binders to the chronically contaminated broiler chicks significantly (p < 0.01) reduced {sup 137}Cs transfer and deposition in breast meat, liver and gizzard. Decreasing deposition of {sup 137}Cs in breast meat and internal organs increased with time of contamination and binders' administration. With AFCF as a cesium binder, on day 13 of measuring the {sup 137}Cs activity in breast meat was 80-83% lower than that in the control group, 89% in liver and 83-84% in gizzard. Natural clinoptilolite demonstrated lower binding efficiency. On day 13 of measuring the {sup 137}Cs activity in breast meat was 53-69% lower than that in the control group, 67-60% in liver and 59-71% in gizzard.

  7. Performance, meat quality, and pectoral myopathies of broilers fed either corn or sorghum based diets supplemented with guanidinoacetic acid.

    Science.gov (United States)

    Córdova-Noboa, H A; Oviedo-Rondón, E O; Sarsour, A H; Barnes, J; Ferzola, P; Rademacher-Heilshorn, M; Braun, U

    2018-04-13

    One experiment was conducted to evaluate the effects of guanidinoacetic acid (GAA) supplementation in broilers fed corn or sorghum-based diets on live performance, carcass and cut up yields, meat quality, and pectoral myopathies. The treatments consisted of corn or sorghum-based diets with or without the addition of GAA (600 g/ton). A total of 800 one-d-old male Ross 708 broiler chicks were randomly placed in 40 floor pens with 10 replicates (20 birds per pen) per each of the four treatments. At hatch, 14, 35, and 50 d, BW and feed intake were recorded. BW gain and FCR were calculated at the end of each phase. Four broilers per pen were selected and slaughtered at 51d and 55d of age to determine carcass and cut up yields, meat quality and myopathies (spaghetti muscle, white striping, and wooden breast) severity in the Pectoralis major. Data were analyzed as a randomized complete block design in a 2 × 2 factorial arrangement with grain type and GAA supplementation as main effects. At 50 d, diets containing GAA improved (P broilers fed corn diets with GAA had higher breast meat yield (P 0.05) by GAA supplementation at any slaughter ages. However, GAA decreased (P broilers supplemented with GAA had double (P broilers fed non-supplemented diets, therefore reducing the severity of this myopathy. In conclusion, GAA supplementation improved broiler live performance in broilers raised up to 50 d independently of grain source, increased breast meat yield in corn-based diets and reduced the severity of wooden breast myopathy.

  8. Efficacy of aniseed extract as immune stimulant and growth promoter in broiler chicks.

    Science.gov (United States)

    Durrani, F R; Sultan, A; Ahmed, Sajjad; Chand, N; Khattak, F M; Durrani, Z

    2007-10-15

    Present research was undertaken to investigate the effect of different level of 6% concentrated (w/v) aniseed extract in broiler chicks at NWFP Agricultural University Peshawar Pakistan. One hundred and sixty, day old broiler chicks were randomly assigned to four treatments, as A, B, C, receiving 20, 30 and 40 mL of 6% (w/v) concentrated aniseed infusion and D was kept as control group. Each treatment was replicated four times with ten chicks per replicate. Chicks were reared in cages in an open sided house. Vaccination was done against ND and IBD. Data were recorded for growth performance, immunity and economics. The data were subjected to statistical analysis, using Completely Randomized Design and MSTATC programme. Mean feed and water intake was nonsignificant (p > 0.05). Mean weight gain, FCR and dressing percentage was found better (p 0.05) in all groups. Mean antibody titer against IBD was higher (p 0.05), while the gross return was found better (p poultry production under different environmental conditions.

  9. Roasted sesame hulls improve broiler performance without affecting carcass characteristics

    Directory of Open Access Journals (Sweden)

    Kamel Z. Mahmoud

    2015-09-01

    Full Text Available An experiment was conducted to evaluate the effect of using graded levels of roasted sesame hulls (RSH on growth performance and meat quality characteristics in broiler chickens. A total of 360 day-old Lohmann chicks were randomly allocated into 24 floor pens and raised over 42 days. One of four dietary treatments was assigned to each group of six pens in a completely randomized fashion. The chicks in the control group were fed a corn-soybean based diet (RSH-0, while the chicks in treatments two, three, and four were fed graded levels of RSH at 4% (RSH-4, 8% (RSH-8, and 12% (RSH-12, respectively. Diets were formulated to meet broiler chicks’ requirements according to the National Research Council for both starter and finisher rations. The results showed that RSH inclusion increased (P<0.05 feed intake and final body weight without adversely affecting the feed conversion ratio. Broiler chicks fed RSH-12 had heavier (P<0.05 breast and leg cuts compared to the control-fed group with no change to their chemical composition. Water holding capacity (WHC, cooking loss (CL, and shear force (SF reported similar results in all dietary groups. The chemical composition of both thigh and breast cuts was not affected by the RSH. After one day of thawing, colour coordinates of breast cuts behaved similarly in all dietary groups. The results of this study suggest that the addition of RSH to broiler diets up to 12% improves their growth performance; nevertheless, carcass characteristics and meat quality showed no alterations compared to the control-fed group.

  10. Influence of Dietary Fat Source on Growth Performance Responses and Carcass Traits of Broiler Chicks

    OpenAIRE

    Mohammadreza Poorghasemi; Alireza Seidavi; Ali Ahmad Alaw Qotbi; Vito Laudadio; Vincenzo Tufarelli

    2013-01-01

    This study was conducted to determine the effects of three different fat sources and their combination on growth performance, carcass traits and intestinal measurements of broiler chickens reared to 42 d of age. Two hundred day-old male broiler chicks (Ross 308) were randomly assigned to one of five treatments with four replicates of 10 chicks based on a completely randomized design. The dietary treatments consisted of 4% added fat from three different sources and their combination as follows...

  11. Haematological and biochemical responses of starter broiler ...

    African Journals Online (AJOL)

    A study was conducted to investigate the haematological and biochemical responses of starter broiler chickens fed copper and probiotics supplemented diets. A total of 180-day old Marshal broiler chicks were randomly allotted to six treatment groups of 30 birds each. The treatments were divided into three replicates of ten ...

  12. Growth indices and cost implications of hybro broiler chicks fed with graded levels of fermented wild cocoyam Colocasia esculenta (L.) Schott corm meal as a replacement for maize.

    Science.gov (United States)

    Olajide, R

    2014-05-01

    Corms such as wild cocoyam [Colocasia esculenta] have potential to replace maize as a cheaper energy source in poultry rations. A feeding trial was conducted to evaluate the effects of graded levels of fermented wild cocoyam [Colocasia esculenta (L.) Schott] corm (FWCC), as substitutes for maize in the diets of broilers at the starter phase. One hundred and twenty unsexed day-old Hybro broiler chicks were randomly distributed to four dietary treatments in a Completely Randomized Design (CRD). There were 3 replicates per dietary treatment with 10 birds per replicate. Diet 1 without FWCC served as the control. Diets 2, 3 and 4 contained 10, 20 and 30% FWCC. Each of the diets represented a treatment. The experimental diets and clean drinking water were supplied ad libitum for 4 weeks (28 days) representing the starter phase of the broiler production. Result of the performance revealed significant (p<0.05) differences in feed intake, weight gain and feed conversion ratio. The economic analysis also showed that cost (Naira58.52) of a kilogram feed was highest (p<0.05) for the control and least (Naira53.10) for 30% FWCC. The least cost (Naira101.24) of feed per kilogram weight gain (p<0.05) was obtained for birds fed 30% FWCC compared to (Naira105.53) for the control. It was concluded that maize can economically be substituted with 30% FWCC in broiler starter diets.

  13. Toxicity of dietary Heliotropium dolosum seed to broiler chickens.

    Science.gov (United States)

    Eröksüz, Y; Eröksüz, H; Ozer, H; Canatan, H; Yaman, I; Cevik, A

    2001-12-01

    Five groups of 20 female broiler chicks were fed different levels of dehulled Heliotropium dolosum seed (w/w%; 0.0, 1.0, 3.0, 5.0 or 10.0%) from 10 to 52 d of age. In all doses the seed caused decreases in daily feed intake, weight gain, and feed efficiency, and biochemical findings, severity of pathologic changes, and mortality rate increased in a dose-dependent manner. Acute toxicity was observed in livers of chicks fed 10% seed. Other test groups had chronic changes. Livers had massive to submassive necrosis, hepatic megalocytosis, bile duct proliferation, fatty change, and periportal fibrosis. Biochemical evaluations revealed hypoalbuminemia, hypoprotienemia and increased ALP activity and billuribin. The seed of Heliotropium dolosum produced biochemical and specific pathologic changes in broiler chicks, as well as decreased food intake and feed efficiency. Higher seed levels induced more pronounced changes.

  14. A STUDY ON THE PATHOGENESIS OF YOLK RETENTION IN BROILER CHICKS

    Directory of Open Access Journals (Sweden)

    Kashif Aziz Khan. Shakeel Akhtar Khan, Shahnaz Hamid2. Asim Aslam and Masood

    2002-04-01

    Full Text Available The present project was designed to identify the factors responsible for yolk retention in broiler chicks. Four different experiments were conducted to find out the effect of four factors (experimental yolk sac infection with Echerichia coli, off-feed period between hatching and housing. first feeding time in the poultry house and type of initial feed on yolk absorption. Results of these experiments showed that yolk sac infection with E. coli led to decrease in rate of yolk absorption. Moisture and protein percentage was increased in infected yolk but fat percentage was not affected by yolk sac infection. On the other hand, post hatch starvation due to delay in housing and delay in first feeding did not affect rate of yolk absorption. Moisture percentage was not affected while fat absorption increased and protein absorption decreased due to fasting. It was also observed that feeding ground corn in early life of chick led to slow absorption of yolk contents as compared to feeding commercial starter ration and 2% sugar solution. Yolk composition was not affected by feeding commercial starter ration, ground corn and 2% sugar solution in early life of chick. It is concluded that experimental infection with E. coli and ground corn as initial feed leads to slow absorption of yolk sac in young broiler chicks.

  15. Effect of Administration of Withania somnifera on Some Hematological and Immunological Profile of Broiler Chicks

    OpenAIRE

    Muhammad Mushtaq*, F. R. Durrani, N. Imtiaz, Umer Sadique1, A. Hafeez, S. Akhtar2 and S. Ahmad3

    2012-01-01

    This study was conducted to assess the performance of broiler chickens based on some hematological and immunological profile, upon inclusion of aqueous extract of Withania somnifera (W. somnifera) in their drinking water. For this purpose, 240 a-day-old broiler chicks were purchased from a local hatchery and divided into four groups i.e. WST-0, WST-I, WST-II and WST-III. Group WST-0 was kept as control, while chicks in group WST-I, WST-II and WST-III were offered in their drinking water with ...

  16. Upgrading the nutritive value of full-fat soyabeans meal for broiler ...

    African Journals Online (AJOL)

    An experiment was conducted using Anak Red broiler chicks to evaluate the effect of grading the nutritive value of full fat soyabean meal diet based with either fishmeal or Blaick soldier fly larvae meal for broiler production. The average live weight gains of broilers fed an all vegetable protein, fishmeal and larvae meal diets ...

  17. Direct fed microbial supplementation repartitions host energy to the immune system.

    Science.gov (United States)

    Qiu, R; Croom, J; Ali, R A; Ballou, A L; Smith, C D; Ashwell, C M; Hassan, H M; Chiang, C-C; Koci, M D

    2012-08-01

    Direct fed microbials and probiotics are used to promote health in livestock and poultry; however, their mechanism of action is still poorly understood. We previously reported that direct fed microbial supplementation in young broilers reduced ileal respiration without changing whole-body energy expenditure. The current studies were conducted to further investigate the effects of a direct fed microbial on energy metabolism in different tissues of broilers. One hundred ninety-two 1-d-old broiler chicks (16 chicks/pen) were randomly assigned to 2 dietary groups: standard control starter diet (CSD) and CSD plus direct fed microbial (DFMD; 0.3%) with 6 pens/treatment. Body weight, feed consumption, whole-body energy expenditure, organ mass, tissue respiration rates, and peripheral blood mononuclear cell (PBMC) ATP concentrations were measured to estimate changes in energy metabolism. No differences in whole body energy expenditure or BW gain were observed; however, decreased ileal O(2) respiration (P energy consumption by PBMC corresponded with an altered immune response, broilers were immunized with sheep red blood cells (SRBC) and assayed for differences in their humoral response. The DFMD-fed broilers had a faster rate of antigen specific IgG production (P direct fed microbial used in this study resulted in energy re-partitioning to the immune system and an increase in antibody production independent of changes in whole body metabolism or growth performance.

  18. Methionine in Velvet Bean ( Mucuna pruriens ) Based Broiler Starter ...

    African Journals Online (AJOL)

    The performance of broiler chicks fed starter diets containing 30% raw or heat treated, and 20% heat treated velvet beans with varying levels of methionine was determined. The influence of varying levels of heat treated velvet beans on growth and carcass characteristics of finishing broilers was also investigated. There was ...

  19. Use of multivitamin, acidifier and Azolla in the diet of broiler chickens.

    Science.gov (United States)

    Islam, M A; Nishibori, M

    2017-05-01

    The experiments were carried out to measure the effect of multivitamin, acidifier and Azolla on growth performance, profitability and lipid profiles of blood of broiler chickens to produce safe and cost effective broilers. In experiment 1, 240 day-old Cobb-500 broiler chicks were fed diets; D 1 (control), D 2 (D 1 with 1 mL multivitamin/liter water), D 3 (D 1 with 1 mL acidifier/liter water), D 4 (D 1 with 1 mL multivitamin and 2 mL acidifier/liter water) having 3 replications in each, and 20 chicks/replication. In experiment 2, 150 day-old Cobb-500 broiler chicks were fed diets; T 1 (control), T 2 (5% Azolla in the diet), T 3 (7% Azolla in the diet) and T 4 (T 1 with 1 mL multivitamin and 1 mL acidifier/liter water) having 3 replications in each, and 20 chicks/replication in control, and 10 chicks/replication in the remaining dietary treatment groups for 35 days. In experiment 1, the highest live weight was observed in D 4 (p0.05). The lowest feed conversion ratio (FCR) (p0.05). However, evidently but not significantly, the highest net profit was obtained in D 2 followed by D 4 , D 1 , and D 3 , respectively. In experiment 2, the highest live weight (p0.05). In conclusion, Azolla and acidifier reduced lipid profiles of broiler chickens. Considering net profit and lipid profiles, 5% Azolla may be the suitable dietary group for producing safe and profitable broilers. However, more studies are needed to confirm this study prior to suggesting using Azolla in the poultry industry.

  20. Influence of housing system, grain type, and particle size on Salmonella colonization and shedding of broilers fed triticale or corn-soybean meal diets.

    Science.gov (United States)

    Santos, F B O; Sheldon, B W; Santos, A A; Ferket, P R

    2008-03-01

    Salmonella colonization in poultry may be influenced by grain type and particle size. Broilers reared either in nonlitter cage-based housing or in a conventionally floored litter house from 0 to 42 d were assigned to 1 of 4 dietary treatments: 1) ground corn-soybean meal (C, 560 microm), 2) coarsely ground corn-soybean meal (CC, >1,700 microm), 3) ground triticale-soybean meal (T, 560 microm), or 4) whole triticale-soybean meal (WT). A 4-strain cocktail of Salmonella enterica was orally gavaged into each chick at placement. Growth performance, cecal and fecal Salmonella populations, gizzard and proventriculus pH, intestinal size, jejunum histomorphometry, and carcass yields were measured. Broilers responded differently to the dietary treatments according to the housing system used. At 42 d, birds reared on litter and fed ground grain had greater BW than those fed coarse grain (2.87 vs. 2.71 kg), whereas cage-reared broilers fed ground triticale were heavier than those fed corn (2.75 vs. 2.64 kg). Broilers raised on litter had a better feed conversion ratio than those raised in cages (1.71 vs. 1.81 g/g). Independent of the housing system, relative eviscerated carcass weights of birds fed T and C were heavier than those of CC- and WT-fed broilers (762 vs. 752 g/kg). Generally, the jejunum villus area and mucosal depth were larger, whereas the small intestine was lighter and shorter in broilers raised on litter. Relative gizzard weights of broilers raised on litter and fed the coarser diets were heavier than those of broilers reared in cages and fed finely ground diets. Feeding whole or coarsely ground grains decreased cecal Salmonella populations in 42-d-old broilers (3.8, 3.9, 4.4, and 4.4 log most probable number/g for CC, WT, C, and T, respectively). Additionally, 42-d-old broilers reared on litter had lower cecal Salmonella populations than those in cages (3.8 vs. 4.4 log most probable number/g). In conclusion, as a feed ingredient, triticale is a good

  1. The occurrence of CTX-M-25-producing Enterobacteriaceae in day-old broiler chicks in Japan.

    Science.gov (United States)

    Yossapol, Montira; Sugiyama, Michiyo; Asai, Tetsuo

    2017-10-07

    Day-old chicks from 3 hatcheries were placed on bedding paper and brought to a commercial broiler farm between January and July 2016. Sixty-six samples of the paper, which were stained with meconium droppings of the chicks, were collected and examined for isolation of cephalosporin-resistant Enterobacteriaceae. Cefotaxime (CTX)-resistant Klebsiella pneumoniae (1 isolate) and Enterobacter cloacae (4 isolates) were isolated from 5 (7.58%) of the 66 samples. Conjugation experiments revealed that the bla CTX-M-25 gene conferring CTX resistance was transferred from the K. pneumoniae isolate and 2 of the 4 E. cloacae isolates to Escherichia coli DH5α via IncA/C plasmids carrying the gene. Our results suggested that the bla CTX-M-25 gene originating from chicks may be spread among commercial broiler farms.

  2. Growth performance, nutrient utilization, and feed efficiency in broilers fed Tithonia diversifolia leaf meal as substitute of conventional feed ingredients in Mizoram.

    Science.gov (United States)

    Buragohain, Rajat

    2016-05-01

    The study was for assessment of growth performance, nutrient utilization, and feed efficiency in broilers fed rations with varying levels of Tithonia diversifolia leaf meal (TDLM) as a substitute of conventional feed ingredients in Mizoram. A total of 180, 1-day-old broiler chicks were randomly divided into six homogeneous groups and fed rations incorporated with TDLM (TDLM at 0% [TDLM-0], 2% [TDLM-2], 4% [TDLM-4], 6% [TDLM-6], 8% [TDLM-8], and 10% [TDLM-10] level as substitute of conventional feed ingredients) for 6 weeks. The chicks were reared in battery brooders for the first 2 weeks, and thereafter, in well-ventilated deep litter house following standard management protocols. Feed and drinking water were provided ad libitum to all the groups throughout the experiment. The daily feed intake and weekly body weight gain were recorded, and a metabolic trial for 3 days was conducted at the end of the 6(th) week. Feed consumption decreased for inclusion of TDLM but without any significant differences, except during the 3(rd) week where it reduced significantly (pbroilers reared under deep litter system of management in Mizoram.

  3. Protection of non-immunized broiler chicks housed with immunized cohorts against infection with Eimeria maxima and E. acervulina

    Science.gov (United States)

    The use of live oocyst vaccines is becoming increasingly important in the control of avian coccidosis in broiler chicks. Knowledge of the mechanisms of how chicks uptake oocysts and become immune is important for optimizing delivery of live vaccines. The current study tests the hypothesis that chick...

  4. Performance and haematological indices of broiler chickens fed ...

    African Journals Online (AJOL)

    The effects of Azadirachta indica (neem), Spondias mombin and Chromolaena odorata leaf meals as natural feed supplements on performance and haematology of broilers were evaluated. Two hundred and sixty-four 1 day-old Arbor acre chicks were divided randomly into six treatments and four replicates. Treatment 1 ...

  5. Arginine and vitamin E improve the immune response after a Salmonella challenge in broiler chicks.

    Science.gov (United States)

    Liu, X; Byrd, J A; Farnell, M; Ruiz-Feria, C A

    2014-04-01

    Two experiments were conducted to evaluate the effects of Arg, vitamin E (VE), and mannanoligosaccharide (MOS) on the immune response and clearance of Salmonella in broiler chickens. In each experiment, 1-d-old chicks (n = 160) were randomly distributed into 4 groups: antibiotic-free diet (negative control, CTL-), antibiotic-supplemented diet (positive control, CTL+), antibiotic free-diet plus Arg and VE (AVE), or antibiotic-free diet plus Arg, VE, and MOS (AVM). Birds were orally challenged with 10(6) cfu of a novobiocyn and nalidixic acid-resistant Salmonella enterica serovar Typhimurium strain at d 7 (experiment 1) or at d 3 (experiment 2). Heterophil- (HOB) and monocyte- (MOB) oxidative burst and lymphocyte proliferation (LPR), antibody titers, and Salmonella content in the ceca were measured at several intervals postinfection (PI). In experiment 1, both AVM and AVE decreased HOB compared with the controls 5 and 9 d PI, but increased LPR 9 d PI. In the same experiment, birds fed the AVE diet had higher MOB than birds fed CTL+ or the AVM diet at 7 d PI, whereas 9 d PI birds fed the AVM diet had the highest MOB. In experiment 2, birds fed the AVE diet had higher MOB, HOB, and LPR than birds in the other treatments 7 and 14 d PI, except at 7 d PI, when MOB was not different among treatments. Birds fed the AVM diet had the highest IgA antibody titer, and a higher IgM antibody titer than the CTL+ birds. In experiment 1, Salmonella Typhimurium content in the ceca was lower in birds fed the AVM diet compared with birds fed the CTL- diet 3 d PI, but later on (10 and 17 d PI), and in experiment 2 (7, 14, and 21 d PI), Salmonella Typhimurium concentrations were not different among treatments. Thus, Arg and VE improved immune response after a Salmonella Typhimurium challenge in young chicks, and although they did not reduce Salmonella Typhimurium concentrations in the ceca, they may improve bacterial resistance against other pathogens in commercial growing conditions.

  6. Blood Parameters, Digestive Organ Size and Intestinal Microflora of Broiler Chicks Fed Sorghum as Partial Substitute of Corn

    Directory of Open Access Journals (Sweden)

    Anca GHEORGHE

    2017-11-01

    Full Text Available A study was conducted to evaluate the effects of dietary white sorghum (WS, as partial substitute of corn on blood parameters, digestive organ size and intestinal microflora of broilers at 35 d. Cobb 500 broilers (n=400, assigned to 2 groups, were fed with isocaloric and isonitrogenous corn-soybean meal control diets (C or corn-WS-soybean meal diets (WS, where corn was partially replaced (50% with WS. At 35 d, sixteen broilers per group were selected for blood sampling, gastrointestinal (GIT measurements, digesta pH and ileum microflora analysis. There was no effect (P>0.05 of the dietary WS inclusion on blood biochemistry parameters, GIT development and digesta pH of broilers. In our study, dietary WS significantly reduced the ileal population of Enterobacteriaceae (-1.38%; P<0.0001 and E. coli (-1.16%; P=0.020, and increased the Lactobacillus spp. (+1.07%; P=0.014 compared with the C diet. In conclusion, feeding white sorghum, as partial substitute of corn in broiler diets, did not affect blood parameters, digestive organ size and digesta pH, that support the obtained performance. In addition, sorghum had a positive effect of the ileal microflora increasing the beneficial bacterial Lactobacillus spp.

  7. Effects of different levels of hatchery wastes on the performance, carcass and tibia ash and some blood parameters in broiler chicks.

    Science.gov (United States)

    Mehdipour, M; Shargh, M Shams; Dastar, B; Hassani, S

    2009-09-15

    This experiment was conducted to evaluate the effects of different levels of Hatchery Wastes (HW) on performance, tibia ash, blood calcium and phosphorus concentrations in broiler chickens. Birds were fed a corn-soybean meal diet for 7 days. The experimental treatments included a corn soybean meal diet and 3 other treatments containing 1.5, 3.0 and 4.5% HW. five replicate groups of 15 Ross 308 broiler chicks were allocated to each dietary treatment. Data were analyzed in a completely randomized design. Results indicated that there were no significant differences in weight gains among different dietary treatments. Feed intake in the 4.5% treatment was significantly higher (p 0.05). Results of carcass analysis showed no significant differences between treatments. Also, there were no significant differences between blood calcium and phosphorous among treatments. The 4.5 and 3.0% hatchery wastes treatments had the highest tibia ash (p hatchery wastes as much as 3% can increase tibia strength without having adverse effect on broilers performance.

  8. Use of multivitamin, acidifier and Azolla in the diet of broiler chickens

    Directory of Open Access Journals (Sweden)

    M. A. Islam

    2017-05-01

    Full Text Available Objective The experiments were carried out to measure the effect of multivitamin, acidifier and Azolla on growth performance, profitability and lipid profiles of blood of broiler chickens to produce safe and cost effective broilers. Methods In experiment 1, 240 day-old Cobb-500 broiler chicks were fed diets; D1 (control, D2 (D1 with 1 mL multivitamin/liter water, D3 (D1 with 1 mL acidifier/liter water, D4 (D1 with 1 mL multivitamin and 2 mL acidifier/liter water having 3 replications in each, and 20 chicks/replication. In experiment 2, 150 day-old Cobb-500 broiler chicks were fed diets; T1 (control, T2 (5% Azolla in the diet, T3 (7% Azolla in the diet and T4 (T1 with 1 mL multivitamin and 1 mL acidifier/liter water having 3 replications in each, and 20 chicks/replication in control, and 10 chicks/replication in the remaining dietary treatment groups for 35 days. Results In experiment 1, the highest live weight was observed in D4 (p0.05. The lowest feed conversion ratio (FCR (p0.05. However, evidently but not significantly, the highest net profit was obtained in D2 followed by D4, D1, and D3, respectively. In experiment 2, the highest live weight (p0.05. Conclusion In conclusion, Azolla and acidifier reduced lipid profiles of broiler chickens. Considering net profit and lipid profiles, 5% Azolla may be the suitable dietary group for producing safe and profitable broilers. However, more studies are needed to confirm this study prior to suggesting using Azolla in the poultry industry.

  9. Nutritional evaluation of low-phytate peas (Pisum sativum L.) for young broiler chicks.

    Science.gov (United States)

    Thacker, Philip; Deep, Aman; Petri, Daniel; Warkentin, Thomas

    2013-02-01

    This experiment determined the effects of including normal and low-phytate peas in diets fed to young broiler chickens on performance, phosphorus availability and bone strength. A total of 180, day-old, male broilers (Ross-308 line) were assigned to six treatments. The control was based on corn and soybean meal while two additional corn-based diets were formulated containing 30% of either normal or low-phytate pea providing 0.45% available phosphorus. For each of these three diets, a similar diet was formulated by reducing the amount of dicalcium phosphate to produce a diet with 0.3% available phosphorus. The total tract apparent availability (TTAA) of phosphorus was higher (p = 0.02) for broilers fed the low-phytate pea than for birds fed the normal pea diets. Birds fed diets containing the lower level of phosphorus had a higher TTAA of phosphorus (50.64 vs. 46.68%) than broilers fed diets adequate in phosphorus. Protein source had no effect on weight gain, feed intake or feed conversion. Broilers fed the low phosphorus diets had lower weight gain (p = 0.04) and feed intake (p pea than for those fed diets based on normal pea or soybean meal. Increasing the availability of the phosphorus in peas could mean that less inorganic phosphorus would be required in order to meet the nutritional requirements of broilers. Since inorganic phosphorus sources tend to be expensive, a reduction in their use would lower ration costs. In addition, increased availability of phosphorus would reduce the amount of phosphorus excreted thus reducing the amount of phosphorus that can potentially pollute the environment.

  10. Effect of dietary nucleotide supplementation on performance and development of the gastrointestinal tract of broilers.

    Science.gov (United States)

    Jung, B; Batal, A B

    2012-01-01

    1. Two experiments were conducted to determine the effects of dietary nucleotide supplementation on broiler performance, and physical and morphological development of the gastrointestinal tract. 2. Experiment 1: A total of 180 one-d-old male chicks were placed in battery brooders in 3 × 6 replicate pens containing 10 chicks each. Chicks were randomly assigned to one of the three dietary treatments; a maize-soyabean meal based diet supplemented with 0, 0·25, and 0·50% Torula yeast RNA (as a source of nucleotides) from 0 to 16 d of age. 3. Experiment 2: A total of 1344 one-d-old male chicks were placed in floor pens and reared on recycled wood shavings (two flocks) under a high stocking density (0·068 m(2)/bird). Chicks were randomly assigned to one of the 4 dietary treatments (0, 0·25% Torula yeast RNA, 2% and 6% Nupro®) for the starter period (0 to 14 d of age) with 6 replicate pens containing 56 chicks each. All the birds were fed on the same common grower diet with no supplementation of nucleotides from 15 to 32 d of age. 4. Experiment 1: Supplementing the diets with up to 0·50% Torula yeast RNA did not affect broiler performance, or relative intestinal tract weight and length of broilers at any periods measured. 5. Experiment 2: From 0 to 14 d of age, broilers fed on the diets supplemented with 0·25% Torula yeast RNA and 2 and 6% Nupro® were significantly heavier and had improved feed conversion (feed:gain) ratios as compared with the birds fed on the control diet. Supplementing the starter diet only with 2% Nupro® supplementation significantly improved body weight (BW) gain as compared with the control diet over the entire experiment (0 to 32 d of age). Broilers fed on the diets supplemented with 2 and 6% Nupro® from 0 to 14 d of age had better feed conversion (feed:gain) ratios over the entire experiment (0 to 32 d of age) as compared with the birds fed on the control diet, even though the birds were only fed on the diets

  11. Effects of Dietary Supplementation with Astaxanthin on Histamine Induced Lesions in the Gizzard and Proventriculus of Broiler Chicks

    Directory of Open Access Journals (Sweden)

    Mi-hyang Ohh

    2016-06-01

    Full Text Available Astaxanthin (ASX is a xanthophyll pigment isolated from crustaceans and salmonids. Owing to its powerful antioxidant activity, ASX has been reported to have the potential to protect against gastric ulcers and a variety of other illnesses. Histamine (His is a dietary factor that causes gastric erosion and ulceration in young chicks. In this study, we examined whether ASX had protective effects on dietary histamine-induced lesions in the gizzard and proventriculus of broiler chickens. Four experimental treatment groups were planned: basal diet (BD, BD+His, BD+ASX, and BD+ASX+His, with four chicks (5 days old in each group and three replications (i.e., a total of 12 chicks per group. The BD was supplemented with either 0.4% His or 100 ppm ASX. The birds were fed ad libitum for 3 weeks, and diets contained no antimicrobial compounds. Supplementing the diet with His significantly decreased body weight gain, but increased the weights of the gizzard and proventriculus of the chicks as compared with those of chicks in the BD group (p<0.05. ASX did not affect His-dependent changes in chick body weight or weights of the gizzard and proventriculus. The loss of gastric glands in the proventriculus, which was observed in His-treated chicks, was not prevented by ASX administration. The frequency of proventricular ulceration, however, was lowered by treatment with ASX, without significant differences between the two supplementation levels. In conclusion, our data showed that ASX might be helpful for alleviating structural damage to the digestive system in poultry under certain stressful conditions.

  12. Effects of Maize Source and Complex Enzymes on Performance and Nutrient Utilization of Broilers

    Directory of Open Access Journals (Sweden)

    Defu Tang

    2014-12-01

    Full Text Available The objective of this study was to investigate the effect of maize source and complex enzymes containing amylase, xylanase and protease on performance and nutrient utilization of broilers. The experiment was a 4×3 factorial design with diets containing four source maize samples (M1, M2, M3, and M4 and without or with two kinds of complex enzyme A (Axtra XAP and B (Avizyme 1502. Nine hundred and sixty day old Arbor Acres broiler chicks were used in the trial (12 treatments with 8 replicate pens of 10 chicks. Birds fed M1 diet had better body weight gain (BWG and lower feed/gain ratio compared with those fed M3 diet and M4 diet (p0.05, respectively. The fresh feces output was significantly decreased by the addition of enzyme B (p<0.05. Maize source affects the nutrients digestibility and performance of broilers, and a combination of amylase, xylanase and protease is effective in improving the growth profiles of broilers fed maize-soybean-rapeseed-cotton mixed diets.

  13. Single and combined effects of vitamin C and oregano essential oil in diet, on growth performance, and blood parameters of broiler chicks reared under heat stress condition

    Science.gov (United States)

    Ghazi, Shahab; Amjadian, Tahere; Norouzi, Shokufeh

    2015-08-01

    This study was conducted to evaluate the effects of adding vitamin C (VC), oregano essential oil (OR), or their combination in diet, on growth performance, and blood parameters of broiler chicks reared under heat stress (HS) condition (38 °C). One-day-old 240 male broilers were randomly assigned to four treatment groups, six replicates of ten birds each. The birds were fed with either a basal diet or a basal diet supplemented with either 200 mg L-ascorbic acid/kg of diet, 250 mg of oregano essential oil/kg of diet, or 200 mg L-ascorbic acid plus 250 mg of oregano essential oil/kg of diet. Average daily feed intake (ADFI), average daily gain (ADG), and feed conversion ratio (FCR) were obtained for 42 days of age and at the end of the experiment (day 42); birds were bled to determine some blood parameters and weighted for final body weight (BW). Feeding birds with diets supplemented with oregano essential oil and vitamin C in a single or combined form increased ADG ( P > 0.05). Also BW increased and feed efficiency decreased ( P vitamin C ( P > 0.05). Supplemental oregano essential oil and vitamin C in a combined form decreased the serum concentration of corticosterone, triglycerides, glucose, and MDA ( P vitamin C were seen in broiler chicks supplemented with vitamin C. From the results of the present experiment, it can be concluded that diet supplementation by combined oregano essential oil and vitamin C could have beneficial effects on some blood parameters of broiler chicks reared under heat stress condition.

  14. Effects of the free fatty acid content in yellow grease on performance, carcass characteristics, and serum lipids in broilers.

    Science.gov (United States)

    Wu, H; Gong, L M; Guo, L; Zhang, L Y; Li, J T

    2011-09-01

    This study was conducted to investigate whether the free fatty acid (FFA) content of yellow grease would influence the performance and carcass characteristics of broiler chicks. A total of 432 one-day-old, male Arbor Acres broilers were randomly allotted to 1 of 4 treatments, with each treatment being applied to 6 pens of 18 chicks. The dietary treatments were based on corn and soybean meal and were supplemented with either soybean oil (2.86% FFA) or yellow grease with a low (2.74%), medium (12.59%), or high (19.05%) FFA content. The fat sources were supplemented at 1.5% of the diet during the starter phase (0 to 21 d) and at 3.0% of the diet during the grower phase (22 to 42 d). From d 0 to 42, the BW gains of chicks fed diets containing soybean oil and yellow grease with 2.74% FFA were similar. As the FFA level in the yellow grease increased, the BW gain of chicks decreased (P dressing percentage and the percentage of breast muscle in the carcass were significantly (P < 0.01) lower for broilers fed any yellow grease diet compared with birds fed soybean oil. In contrast, abdominal fat was significantly increased in diets containing yellow grease. These results demonstrate that the performance of birds fed yellow grease with a low content of FFA was essentially equal to that of birds fed soybean oil. However, because yellow grease samples containing FFA levels greater than 2.74% negatively affected bird performance, producers should exercise caution with regard to feeding broilers yellow grease with an elevated FFA content. In 42-d-old broilers, serum total cholesterol and low-density lipoprotein cholesterol levels were elevated in birds fed yellow grease, regardless of the dietary level. In contrast, serum high-density lipoprotein cholesterol and triglyceride levels were unaffected by dietary treatment. Although dietary FFA may influence triglyceride-rich lipoprotein metabolism in broilers, an explanation for the observed effects remains elusive.

  15. Effect of citric acid, avilamycin, and their combination on the performance, tibia ash, and immune status of broilers.

    Science.gov (United States)

    Chowdhury, R; Islam, K M S; Khan, M J; Karim, M R; Haque, M N; Khatun, M; Pesti, G M

    2009-08-01

    The aim of this study was to determine the effect of the supplementation of an organic acid (citric acid), antibiotic growth promoter (avilamycin), and their combination for a period of 35 d on the growth, feed efficiency, carcass yield, tibia ash, and immune status of broilers. One hundred sixty 1-d-old broiler chicks (Hubbard Classic) were randomly distributed into 4 groups with 4 replicate cages having 10 birds in each. A corn-soybean-based diet was used as the basal diet (control). The basal diet was supplemented with an organic acid (citric acid, 0.5%), an antibiotic growth promoter (avilamycin, 0.001%), and their combination in other groups. The highest BW was attained in citric acid-fed chicks (1,318 g), which was significantly (P 0.05). Total feed intake was higher in citric acid-fed chicks compared with antibiotic-supplemented chicks. The addition of citric acid improved feed conversion efficiency (g of weight gain/ kg of feed intake) significantly (P ash percentage significantly (P ash, and immune status of broilers. Therefore, citric acid might be a useful additive instead of antibiotic growth promoters such as avilamycin, considering performance and health status of broilers.

  16. Irradiated eggs and chicks in broilers: 3- carcass and blood parameters

    International Nuclear Information System (INIS)

    Shebaita, M.K.; Abdelaal, A.E.; Fahmy, M.O.; Ezzat, I.E.; EL-Barkouky, E.E.; Salem, M.A.I.

    1991-01-01

    This study was carried out on 200 broiler chicks (Hcbbard) representing 3 groups of non-irradiated, irradiated eggs and 4-day-old irradiated chicks (exposed to gamma rays of Co-60). The doses used were 500 and 1000 rad.Half the chickens within each group and within each dose level were treated with vitamin C (50 mg/chick/day) in the drinking water. Carcass analysis, giblets weight and some blood parameters were measured individually in five chicks from each group at 2,4,5 and 6 weeks of age. The data revealed that the exposure of either eggs or chicks to gamma-rays induced a highly significant decrease in liver, heart and gizzard weights. The effect was more pronounced in the irradiated chicks than that in the chicks of irradiated eggs particularly at the dose level of 1000 rad. Except few sporadic cases noticed at different age intervals, no appreciable changes in either carcass chemical analysis (water, fat, protein and ash contents) or blood parameters (RBCs, Hb, PCV, Ca, P, cholesterol, total protein, albumin and globulin) were observed. It seems that the giblets were more sensitive to and reflect the hazardous effects of ionizing radiation than carcass chemical analysis and/or Blood parameters

  17. Effect of Administration of Withania somnifera on Some Hematological and Immunological Profile of Broiler Chicks

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    Muhammad Mushtaq*, F. R. Durrani, N. Imtiaz, Umer Sadique1, A. Hafeez, S. Akhtar2 and S. Ahmad3

    2012-01-01

    Full Text Available This study was conducted to assess the performance of broiler chickens based on some hematological and immunological profile, upon inclusion of aqueous extract of Withania somnifera (W. somnifera in their drinking water. For this purpose, 240 a-day-old broiler chicks were purchased from a local hatchery and divided into four groups i.e. WST-0, WST-I, WST-II and WST-III. Group WST-0 was kept as control, while chicks in group WST-I, WST-II and WST-III were offered in their drinking water with an extract of W. somnifera at 10, 20 and 30g/L for 35 days, starting from day 1 of age. Feed intake and body weight were recorded on regular basis and hematological and immunological profile of the birds was analyzed at the end of experiment. Administration of W. somnifera extract resulted in significantly higher feed intake and body weight of birds in all treated groups. An increase in the values of Hb, PCV and TLC was noted in the treatment groups. A non significant difference was noted in the counts of neutrophils, lymphocytes, eosinophils and monocytes in all treatment groups as compared to control. Antibodies titers against IB and IBD were significantly higher in all treatment groups as compare to the values shown by the chicks in control group. Findings of this study indicated that administration of W. somnifera extract to broiler chicks improve their feed intake, body weight gain, hematological profile and immunological status.

  18. Performance characteristics of broilers fed graded levels of Moringa oleifera leaf meal

    OpenAIRE

    Ayo-Ajasa, O.Y.; Abiona, J.A.; Fafiolu, A.O.; Egbeyale, L.T.; Njoku1, C.P.; Omotayo1, I.G.; Odeyemi1, A.Y.; Abel, F.A.S.

    2016-01-01

    Cost of conventional protein sources is on the increase recently; hence, there is the need for cheaper alternative sources that will not compromise the performance characteristics of broiler birds taking into consideration the cost of production. Moringa leaf meal has been reported to increase the performance of broiler birds due to its rich protein content. Two hundred day-old broiler chicks were used to assess the effects of partial replacement of soya bean meal with Moringa (Moringa oleife...

  19. Beneficial effects of adding lipase enzyme to broiler diet

    International Nuclear Information System (INIS)

    Elbarkouky, E.M.A.

    2005-01-01

    A total number of 300 Ross broiler chicks were obtained from commercial hatchery at one day of age. The chicks were divided into three groups (50 males and 50 females in each). The first and second groups were supplemented with 3000 and 2000 lU/kg diet of lipase enzyme, respectively, while the third group served as control and fed on basal diet. Birds fed on diets that supplemented with lipase enzyme showed significant increase in body weight and dry matter intake, as well as fats and protein content dry matters. The serum lipase activity showed significant increase in treated groups compared to the control. Non-significant changes were determined in serum total lipids, T3, T4 and ash content. Birds supplemented with lipase showed significant decrease in cholesterol concentration. It could be concluded that birds fed diets containing 2000 or 3000 lU/kg diet of lipase enzyme exhibited improvement in broiler performance

  20. Evaluation of camel rumen content as a feed for broiler chickens

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    O. J. Makinde

    2017-06-01

    Full Text Available This study was carried out to evaluate the growth performance, carcass characteristics and haematological parameters of broiler chickens fed diets containing graded levels of Camel Rumen Content (CRC as a replacement for maize and groundnut cake. CRC was included in the diets of broilers at 0, 5, 10, 15, and 20% and designated as TI, T2, T3, T4 and T5 respectively. Five dietary treatments were formulated. One hundred and fifty day-old broiler chicks (Anak breed were randomly allotted to five treatments replicated thrice with 10 chicks per replicate in a completely randomized design. Feed and water were supplied ad libitum for 28 days. Means of body weight gain, feed intake and feed conversion ratio of broilers fed the control diet, 5%, 10% and 15 % CRC diets were significantly (P 0.05 differences in the haematological parameters measured except haemoglobin and mean corpuscular haemoglobin concentration. Also, basophils and monocytes were not significantly (P>0.05 different among the differential counts measured. It was concluded that up to 15% CRC can be included in broilers diet to replace maize and groundnut cake without adverse effect on the performance of birds.

  1. EFFICACY OF GENTAMICIN AFTER INTRAYOLK ADMINISTRATION IN EXPERIMENT ALLY INDUCED OMPHALITIS IN BROILER CHICKS

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    Muhammad Ashraf, Qamar Arif and Kashif Aziz Khan1

    2002-04-01

    Full Text Available A study was conducted to observe the effect of intrayolk injection of gentamicin on experimentally induced omphalitis in broiler chicks. Escherichia coli was injected into yolk sac to induce omphalitis and treatment was done by intrayolk injection of gentamicin. Results showed that mortality decreased and feed intake increased after treatment, Intrayolk injection of antibiotics is suggested as an alternative to oral administration for the treatment of omphalitis in chicks.

  2. Interleukin-10 neutralizing antibody for detection of intestinal luminal levels and as a dietary additive in Eimeria challenged broiler chicks.

    Science.gov (United States)

    Arendt, Maria K; Sand, Jordan M; Marcone, Taylor M; Cook, Mark E

    2016-02-01

    Interleukin-10 (IL-10) mRNA levels are increased within intestinal mucosa after Eimeria infection. IL-10 apical receptor presence on enterocytes suggests IL-10 is secreted into the intestinal lumen. Increased IL-10 has been shown to be central to the pathogenesis of numerous intracellular pathogens; we hypothesize luminal secretion of IL-10 enables Eimeria spp. infection in chickens. This study examines intestine luminal IL-10 levels and performance in broilers challenged with Eimeria when fed an anti-IL-10 antibody. Chicks were fed a diet (1 to 21 d) with control or anti-IL-10 antibody (0.34 g egg yolk antibody powder/Kg diet) with a saline or 10× dose of Advent coccidiosis vaccine on d 3. One chick per pen was euthanized on days 2, 4, 7, 10, 13, 16, and 19 post-challenge, bled, and intestines were collected for luminal fluid IL-10 concentrations. Body weight and feed intake were measured on d 21, and oocyst shedding was assessed on d 7 post-challenge. A significant Eimeria × antibody interaction on d 21 body weight (P < 0.05) showed chicks fed control antibody, but not anti-IL-10, had significant reductions in body weight when challenged with Eimeria spp. Oocyst shedding was increased with Eimeria challenge, but dietary antibody had no effect. Plasma carotenoid levels were reduced in Eimeria challenged chicks 4, 7, 10, and 16 days post-challenge compared to unchallenged chicks. Lack of an Eimeria × antibody interaction showed anti-IL-10 was not protective against Eimeria-induced decreases in plasma carotenoids. Eimeria challenge increased intestine luminal IL-10 on days 4 and 7 post-challenge in the cecum and jejunum, respectively, compared to unchallenged. Dietary anti-IL-10 decreased luminal IL-10 in the ileum on day 2 post-challenge when compared to control antibody fed chicks. No interaction between Eimeria challenge and antibody was observed on intestine luminal contents of IL-10, suggesting anti-IL-10 was ineffective at preventing increased Eimeria

  3. Effects of Protease, Phytase and a Bacillus sp. Direct-Fed Microbial on Nutrient and Energy Digestibility, Ileal Brush Border Digestive Enzyme Activity and Cecal Short-Chain Fatty Acid Concentration in Broiler Chickens

    Science.gov (United States)

    Murugesan, Ganapathi R.; Romero, Luis F.; Persia, Michael E.

    2014-01-01

    Two experiments were conducted to determine the effects of protease and phytase (PP) and a Bacillus sp. direct-fed microbial (DFM) on dietary energy and nutrient utilization in broiler chickens. In the first experiment, Ross 308 broiler chicks were fed diets supplemented with PP and DFM in a 2×2 factorial arrangement. The 4 diets (control (CON), CON + PP, CON + DFM, and CON + PP + DFM) were fed from 15–21 days of age. In Experiment 1, significant interaction (P≤0.01) between PP and DFM on the apparent ileal digestibility coefficient for starch, crude protein, and amino acid indicated that both additives increased the digestibility. Both additives increased the nitrogen retention coefficient with a significant interaction (P≤0.01). Although no interaction was observed, significant main effects (P≤0.01) for nitrogen-corrected apparent ME (AMEn) for PP or DFM indicated an additive response. In a follow-up experiment, Ross 308 broiler chicks were fed the same experimental diets from 1–21 days of age. Activities of ileal brush border maltase, sucrase, and L-alanine aminopeptidase were increased (P≤0.01) by PP addition, while a trend (P = 0.07) for increased sucrase activity was observed in chickens fed DFM, in Experiment 2. The proportion of cecal butyrate was increased (P≤0.01) by DFM addition. Increased nutrient utilization and nitrogen retention appear to involve separate but complementary mechanisms for PP and DFM, however AMEn responses appear to have separate and additive mechanisms. PMID:25013936

  4. The effects of particle size and origin of calcium carbonate on performance and ossification characteristics in broiler chicks.

    Science.gov (United States)

    Guinotte, F; Nys, Y; de Monredon, F

    1991-09-01

    The following physico-chemical characteristics of various calcium sources, differing in origin and particle size were determined: mineral composition, sieve and image analysis, apparent solubility (AS), surface area (SA), porous volume, specific gravity, and compressibility (C). The AS, SA, and C values were related more to the calcium particle size than to its origin and were higher in ground calcium sources. Calcium retention of seashells treated with phosphoric acid, oyster shells, and limestone using two particle sizes, ground or particulate, was assayed in 98 broiler chicks. Particulate marble was also tested in this experiment. Calcium retention expressed as a percentage of calcium ingestion was decreased when coarse particles of calcium were supplied in lieu of pulverized sources (40 versus 49%). An experiment with a 3 x 3 x 3 factorial arrangement of treatments were tested using 576 broiler chicks. Treatments included three calcium sources (phosphorus-treated shell, oyster shell, and marble), three particle sizes [ground (less than .15 mm), medium (.6 to 1.18 mm) and coarse (greater than 1.18 mm)] and three levels of calcium (.5,.7, and .9%) with ground limestone as a reference. Performance, tibial morphometry, breaking strength variables, and ash content were measured at 4 wk of age. Weight gain and feed conversion were ameliorated with ground particles of calcium. Cortical thickness, length of the tibia, stiffness, stress, and tibia ash were diminished when coarse particles of calcium were incorporated in the diets. Conversely, the origin of the calcium source hardly affected these criteria. Additionally, the incorporation of phosphorus-treated shells was assayed in 112 broiler chicks. Coarse particles decreased calcium retention. Consequently, ultimate stress, the modulus of elasticity, and stress were impaired. It is concluded that differences in utilization of calcium carbonate sources by the broiler chick is primarily a result of particle size rather

  5. Effect of feed texture on growth performance, dressing percentage and organ weight of broilers

    International Nuclear Information System (INIS)

    Mahmood, S.; Altaf, H.; Hassan, M.M.U.

    2013-01-01

    Comparative efficacy of two important forms of feed, mash and crumbles, fed alone or in combination (mash-crumbs), was studied on growth performance, dressing percentage and organ weight of broilers. One hundred twenty broiler chicks were used in the present study and were fed mash, crumbles and mash-crumbs feed for 0-6 weeks of age. Four treatments, designated as A, B, C and D were used in this experiment. Chicks in group A were kept on mash feeding serving as control and those in group B were offered crumbles. Group C was fed mash from day old to two weeks and crumbles from three to six weeks of age while group D was offered mash from day old to four weeks and then crumbles were fed for next two weeks of age. The results of the experiment showed that different forms of feed exhibited significant (P<0.05) effect on overall weight gain and feed conversion ratio (FCR) of the broilers. Whereas, feed consumption, dressing percentage and relative weights of liver, heart, gizzard, spleen, pancreas, intestine and abdominal fat pad of the birds remained unaffected due to different forms of feed. The broilers maintained upon crumbles throughout the experimental period, fetched maximum profit than other treatment groups. (author)

  6. Effects of Dietary Additives and Early Feeding on Performance, Gut Development and Immune Status of Broiler Chickens Challenged with

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    Z. Ao

    2012-04-01

    Full Text Available The effects of dietary additives and holding time on resistance and resilience of broiler chickens to Clostridium perfringens challenge were investigated by offering four dietary treatments. These were a negative control (basal, a positive control (Zn-bacitracin and two dietary additives, mannanoligosaccharides (MOS, and acidifier. Two holding times included (a immediate access to feed and water post hatch (FED and (b access to both feed and water 48 h post hatch (HELD. Chicks fed Zn-bacitracin had no intestinal lesions attributed to necrotic enteritis (NE, whereas chicks fed both MOS or acidifier showed signs of NE related lesions. All dietary treatments were effective in reducing the numbers of C. perfringens in the ileum post challenge. The FED chicks had heavier body weight and numerically lower mortality. The FED chicks also showed stronger immune responses to NE challenge, showing enhanced (p<0.05 proliferation of T-cells. Early feeding of the MOS supplemented diet increased (p<0.05 IL-6 production. The relative bursa weight of the FED chicks was heavier at d 21 (p<0.05. All the additives increased the relative spleen weight of the HELD chicks at d 14 (p<0.05. The FED chicks had increased villus height and reduced crypt depth, and hence an increased villus/crypt ratio, especially in the jejunum at d 14 (p<0.05. The same was true for the HELD chicks given dietary additives (p<0.05. It may be concluded that the chicks with early access to dietary additives showed enhanced immune response and gut development, under C. perfringens challenge. The findings of this study shed light on managerial and nutritional strategies that could be used to prevent NE in the broiler industry without the use of in-feed antibiotics.

  7. Effects of a combined hatching and brooding system on hatchability, chick weight, and mortality in broilers.

    Science.gov (United States)

    van de Ven, L J F; van Wagenberg, A V; Groot Koerkamp, P W G; Kemp, B; van den Brand, H

    2009-11-01

    Chicks hatch over a time window of approximately 36 to 48 h and are removed from the hatchers only when the majority of the chicks has hatched. Consequently, chicks are exposed to prolonged posthatch holding periods and delays in feed and water access, leading to dehydration and impaired posthatch performance. It is questionable whether the physiological requirements of the hatchlings can be met with current hatching systems. An alternative system that may better match the requirements of the hatchlings is a system that combines the hatching and brooding phase, so that feed and water can be provided immediately after hatch. Such a system, named Patio, was developed in the Netherlands and tested from 2006 to 2008, to evaluate effects on hatchability and early performance of broilers. This paper describes the Patio system and the results from these tests. A total of 21 broiler production trials (780,686 eggs) in the Patio system were evaluated at 3 locations and compared with control hatches of eggs of the same parental flock in the hatchery. Hatchability in the Patio was on average 1.45, 1.83, and 1.86% higher at location 1, 2, and 3, respectively. However, in the calculation of the hatchability in the Patio, possible second grade chicks were included, whereas these were excluded in the calculation of hatchability in the hatchery. Additionally, in the hatchery, the hatching process was interrupted earlier than in the Patio, meaning that possible late hatching chicks remained in the flock in the Patio, but not in the hatchery. In 3 trials, the Patio chicks were 11.6 to 16.3% heavier at d 0, when the hatchery chicks were placed in the broiler house. Mean cumulative 7-d mortality was only assessed in the Patio and was 1.27, 1.09, and 1.43% at location 1, 2, and 3, respectively. The Patio system appears to function as an alternative to current hatching and brooding systems. Further studies are required to determine to what extent the higher hatchability is due to second

  8. Fatty acids profile and quality characteristics of broiler chicken meat fed different dietary oil sources with some additives

    Directory of Open Access Journals (Sweden)

    Engy Fayz Zaki

    2018-04-01

    Full Text Available The study was carried out to investigate the effect of feeding broiler chicken on different vegetable oils with feed additives on the quality characteristics of chicken meat. A total of 216 one-day-old chicks of (Hubbard strain were randomly assigned to six dietary treatments as (2×3 factorial designs where two sources of dietary oil with three levels of commercial multi-enzyme feed additives. Treatments were: soybean oil only (T1, soybean oil+ ZAD (T2, soybean oil+ AmPhi-BACT (T3, palm oil only (T4, palm oil + ZAD (T5 and palm oil + AmPhi- BACT (T6.  Results showed that feeding broiler chicken on different types of dietary oils had significant effect on the fatty acid profile of broiler chicken meat. UFA/SFA ration of broiler chicken groups (T4, T5adT6 were significantly lower compared with (T1, T2 and T3 groups. Broiler fed on soybean oil had significantly higher n-6: n-3 ration compared with broiler fed on palm oil. Regardless of the source of dietary oil, significant differences were observed in the most of fatty acid profile in the chicken meat among levels of commercial multi- enzyme feed additives. Meat of T5and T6 had the higher pH value, followed by meat of T1and T3 groups, while the lowest pH value found in meat of T2 and T4. The higher cooking loss was found in meat of T4 while, meat of T5had the lowest value. Data of chilling loss indicated that the differences between dietary treatments were not significantly different except for meat of T6 which had the higher chilling loss. No significant differences were found in color measurements between dietary treatments.

  9. Efficacy of pine leaves as an alternative bedding material for broiler chicks during summer season

    Directory of Open Access Journals (Sweden)

    Gourav Sharma

    2015-10-01

    Full Text Available Aim: The aim was to assess the efficacy of pine leaves as an alternative bedding material on the performance of broiler chicks. Materials and Methods: The present study was conducted in summer. Total 120, day old Vencobb straight run chicks were procured, and after 5 days of brooding, chicks were randomly distributed into four treatment groups viz. paddy husk (Group I, paddy straw (Group II, pine leaves (Group III, and combination of paddy straw and pine leaves (Group IV, each having 30 chicks with 3 replicates of 10 chicks each. Chicks were reared under intensive conditions in houses that have a semi-controlled environment, with optimum temperature and adequate ventilation. Food and water were provided as per NRC (1994 requirement. Results: The average body weight after 6 weeks of the experiment was 2018.83±31.11, 1983.80±33.27, 2007.36±35.73, and 1938.43±36.35 g. The bedding type had no significant effect on the carcass characteristics viz. evisceration rate and proportion of cut-up parts of the carcass except giblet yield. The experiment suggested that performance of broiler chicks reared on paddy straw and pine leaves as litter material, had improved body weight and feed conversion ratio as compared to rearing on paddy husk as bedding material. Bacterial count, parasitic load and the N, P, K value of manure of different bedding material shows no significant difference. Conclusion: Pine leaves have a potential to be used as an alternative source of litter material to economize poultry production in a sustainable way, so as to make poultry farming as a profitable entrepreneur.

  10. Efficacy of pine leaves as an alternative bedding material for broiler chicks during summer season.

    Science.gov (United States)

    Sharma, Gourav; Khan, Asma; Singh, Surender; Anand, Ashok Kumar

    2015-10-01

    The aim was to assess the efficacy of pine leaves as an alternative bedding material on the performance of broiler chicks. The present study was conducted in summer. Total 120, day old Vencobb straight run chicks were procured, and after 5 days of brooding, chicks were randomly distributed into four treatment groups viz. paddy husk (Group I), paddy straw (Group II), pine leaves (Group III), and combination of paddy straw and pine leaves (Group IV), each having 30 chicks with 3 replicates of 10 chicks each. Chicks were reared under intensive conditions in houses that have a semi-controlled environment, with optimum temperature and adequate ventilation. Food and water were provided as per NRC (1994) requirement. The average body weight after 6 weeks of the experiment was 2018.83±31.11, 1983.80±33.27, 2007.36±35.73, and 1938.43±36.35 g. The bedding type had no significant effect on the carcass characteristics viz. evisceration rate and proportion of cut-up parts of the carcass except giblet yield. The experiment suggested that performance of broiler chicks reared on paddy straw and pine leaves as litter material, had improved body weight and feed conversion ratio as compared to rearing on paddy husk as bedding material. Bacterial count, parasitic load and the N, P, K value of manure of different bedding material shows no significant difference. Pine leaves have a potential to be used as an alternative source of litter material to economize poultry production in a sustainable way, so as to make poultry farming as a profitable entrepreneur.

  11. Graded levels of sugar syrup in broiler rations and its effect on growth performance and blood biochemical parameters

    Directory of Open Access Journals (Sweden)

    Ahmed S. Hussein

    2016-09-01

    Full Text Available Dietary energy for chickens normally includes cereal grains and fat. This innovative study investigated the effect of replacing part of the corn and fat in broiler chicken rations with graded levels of sugar syrup on growth performance and biochemical parameters. Experimental treatments consisted of feeding a corn-soy basal diet alone, or with graded levels of sugar syrup in increments of 5%, 10% and 15%. All starter diets were isonitrogenous and isocaloric. Body weight gain and efficiency of feed utilization of chicks fed the control diet alone were not significantly (P < 0.05 different from chicks fed diets supplemented with either 5% or 15% sugar syrup. Supplementation of sugar syrup to broiler diets had no significant effect on blood glucose, creatinine, total protein, or liver enzymes. Adding 5% sugar syrup to broiler rations significantly decreased blood cholesterol and triglycerides in chickens fed the sugar syrup diet compared with birds fed the control diet. In conclusion, the results shows sugar syrup can be used in poultry ration to replace part of the corn as a source of energy. These results allowed the authors to recommend the safe usage of sugar syrup in broiler rations.

  12. Monitoring phthalates in the tissues of broiler chicks with feedstuffs different phthalate contents

    Directory of Open Access Journals (Sweden)

    Alžbeta Jarošová

    2010-01-01

    Full Text Available For the monitoring of distribution and accumulation of phthalic acid esters (PAE in animal tissues, samples of muscle, mesenteric fat (fat, skin and liver from broiler chicks ROSS 308 were used. The chicks were divided into 4 groups (50 chicks each. All the chicks were given commercial diets (complete feed, KKS for broiler chicks (starter – BR1; grower – BR2 and finisher – BR3. The experimental diets were supplemented with vegetable oil (RO with low (group N or high (group V phthalate contents, or animal fat with a high phthalate content (group Z. Neither the control diets (K, nor the grower (BR1 diets contained vegetable oils or animal fat. The N chicks were given the grower (BR2 and finisher (BR3 diets supplemented with 5% and 3% vegetable oil, respectively. The V chicks were given BR2 and BR3 diets with 5% and 3% vegetable oil, respectively. The Z chicks were given BR2 and BR3 diets with 5% and 3% animal fat, respectively. Di-n-butyl phthalate (DBP and di-(2-ethylhexyl phthalate (DEHP were found in the tissues of chicks in all the experimental groups. The DBP content in the muscle ranged from 0.03 to 0.55 mg . kg−1, in the adipose tissue from < 0.20 to 2.56 mg . kg−1, in the skin from < 0.20 to 1.49 mg . kg−1, and in the liver from 0.03 to 0.13 mg . kg−1. The content of DEHP in the muscle ranged from 0.03 to 1.15 mg . kg−1, in the adipose tissue from 0.25 to 9.85 mg . kg−1, in the skin from < 0.20 to 4.68 mg . kg−1, and in the liver from 0.16 to 0.24 mg . kg−1. The highest concentrations of DBP of 1.28 ± 1.00 mg . kg−1 of fresh sample (an average value from 8 chicks was determined in the adipose tissue of V chicks. The highest concentration of DEHP of 3.27 ± 2.87 mg . kg−1 of fresh sample (mean of 8 chicks was also determined in the V group. The accumulation of DEHP was 3.2; 2.6 and 2.9 times higher than that of DBP in the muscle, adipose tissue and skin

  13. Date Fiber as a Constituent of Broiler Starter Diets

    Directory of Open Access Journals (Sweden)

    W. AI-Marzooki

    2000-06-01

    Full Text Available In Oman, efforts are underway to find alternative sources of fwd to support domestic livestock. The date industry thrives in Oman and one of its by-products is date fiber, a by-product of date syrup production. Although the product contains some free sugars, an evaluation of its potential in broiler diets has never been undertaken. Therefore, we conducted an experiment to investigate the value of date fiber in broiler chick diets. The experimental diets consisted of four graded levels of date fiber: 0, 15, 30 and 45 % in a broiler-starter ration replacing corn as an energy source. The diets were isonitrogenous. There were five replicates for each of the four diets and each replicate contained six broiler chicks. As date fiber percent increased in the dietary treatment, there were linear reductions in body weight gain, calculated apparent metabolizable energy (AMEn content, and apparent protein digestibility (P < 0.05. Poor feed utilization (i .e., reduced feed efficiency was observed in birds fed date fiber diets compared to the control group (P < 0.05. The poor performance of birds fed date fiber may be attributed to protein and energy limitations. We conclude that date fiber has no value in growing broiler diets. However, it may have utility in diets where a stable body weight is desirable.

  14. The effect of refined functional carbohydrates from enzymatically hydrolyzed yeast on the transmission of environmental Salmonella Senftenberg among broilers and proliferation in broiler housing.

    Science.gov (United States)

    Walker, G K; Jalukar, S; Brake, J

    2018-04-01

    Hatching eggs collected from resident broiler breeders at 48 wk of age were used to produce male and female chicks that were assigned sex separately to 96 new litter pens and fed either a 0 or 50 g/MT RFC (refined functional carbohydrate feed additive derived from yeast) diet. There were 24 replicate pens of 12 broilers each per diet per sex. Feed intake and BW were determined at 14, 28, and 42 d of age. Litter was sampled by pen using sterile socks at 35 d and tested for Salmonella spp. using an enzyme linked fluorescence assay method. Salmonella spp. was isolated from 7 of 48 control-fed broiler pens but no RFC-fed pens (P ≤ 0.05). Thereafter, 48 males and 48 females were selected based on litter Salmonella presence and RFC treatment. The cecas of these broilers were aseptically excised after feed withdrawal and lairage and tested for presence of Salmonella spp. There were 18 of the 48 control-fed broilers confirmed positive from litter-positive pens but none from litter-negative pens fed RFC. The serovar of litter and cecal Salmonella isolates was Salmonella enterica subsp. enterica serovar Senftenberg (S. Senftenberg). Female broilers that were fed RFC exhibited greater BW at 28 d (P ≤ 0.05) and 42 d (P ≤ 0.05) while RFC-fed males exhibited improved feed efficiency during the 15-28 d period (P = 0.06). These data demonstrated that dietary RFC reduced the prevalence of Salmonella in the litter and ceca of broilers when fed continuously while not being detrimental to broiler live performance.

  15. Effect of dietary supplementation of Shatavari (Asparagus racemosus wild On heamatobiochemical parameters of broilers

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    Rekhate

    2010-12-01

    Full Text Available Eighty, day old straight run commercial broilers chicks were randomly distributed into four groups (T1, T2, T3 and T4 of two replications having ten chicks in each, reared on deep litter system upto 8 weeks of age. The chicks in all the treatment groups were fed basal diet T1 control and T2, T3 and T4 groups were fed basal diet + 0.5, 1.00 and 1.5 per cent Shatavari root powder (SRP respectively. Significant (P<0.01 improvement in live body weight of broilers was observed between the treatments. Better FCE was observed with the increase in SRP. The digestibility of protein found significantly (P< 0.01 better with higher inclusion level of SRP. Hb, total serum protein, albumin and globulin revealed significant (P< 0.01 variation between the treatments. It was concluded that supplementation of Shatavari root powder at 0.5, 1.00 and 1.5 per cent level improves growth performance and general health status of broilers however, 1.0 per cent level could be more economical. [Vet. World 2010; 3(6.000: 280-281

  16. LEVAMISOLE TOXICOSIS IN BROILER CHICKS SUFFERING FROM SUBACUTE TOXICOSIS OF LEAD, SELENIUM OR MONENSIN

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    Jozef Szarek, Muhammad Zargham Khan1 and Jerzy Szenfeld2

    2001-02-01

    Full Text Available Broiler chicks of 2 weeks of age were grouped and fed lead (1200 mg/kg feed, selenium (15 mg/kg feed, selenium plus vitamin E (15 + 200 mg/kg feed and monensin (240 mg/kg feed to induce subacute toxicosis. One group was kept on basal feed. After four weeks the first subgroup from each group was perorally given 250 mg levamisole/kg body mass, the second subgroup was subcutaneously administered 100 mg levamisole/kg body mass and the third subgroup was given no treatment. The oral administration of levamisole did not produce any clinical signs. The subcutaneous administration of levamisole resulted in shivering, partial or complete paralysis and death in different groups. The higher number of death and severe clinical signs following levamisole subcutaneous administration were observed in birds subacutely intoxicated with lead, selenium and monensin compared with control group. This observation suggests that subacute toxicosis of these substances may alter the clinical pattern of levamisole toxicosis.

  17. Immunological evaluation of the intestinal mucosa of broiler chicks treated with Lactobacillus Spp. and challenged with Salmonella Enteritidis

    Directory of Open Access Journals (Sweden)

    AS Okamoto

    2007-12-01

    Full Text Available This study aimed at the antibody production by intestinal mucosa of broilers chicks were orally inoculated with Lactobacillus spp. at one and/or 21 days of age, and subsequently challenged with Salmonella enterica, subspecies enterica, serotype Enteritidis (S. Enteritidis. A total number of 288 drug-free broiler chicks was divided into 6 groups (groups A, B, C, D, E, and F, according to age at Lactobacillus spp. inoculation and SE challenge. The intestinal mucosa immune response was determined as the production of immunoglobulin A against S. Enteritidis, and evaluated by the Enzyme-Linked Immunosorbent Assay (ELISA technique in intestinal washing fluid. Groups treated with Lactobacillus spp. presented higher IgA production only when the chicks were challenged with S. Enteritidis at 21 days of age. Nevertheless, the expected stimulus for intestinal mucosa antibody production induced by Lactobacillus spp was observed in only some of the treated groups, demonstrating that the protocol utilized in the present experiment resulted in few beneficial effects for chicks, particularly during the first days of life.

  18. Evaluation of camel rumen content as a feed for broiler chickens

    OpenAIRE

    O. J. Makinde; A. M. Abdullahi; G. Mohammed

    2017-01-01

    This study was carried out to evaluate the growth performance, carcass characteristics and haematological parameters of broiler chickens fed diets containing graded levels of Camel Rumen Content (CRC) as a replacement for maize and groundnut cake. CRC was included in the diets of broilers at 0, 5, 10, 15, and 20% and designated as TI, T2, T3, T4 and T5 respectively. Five dietary treatments were formulated. One hundred and fifty day-old broiler chicks (Anak breed) were randomly allotted to fiv...

  19. Evaluation of kefir as a potential probiotic on growth performance, serum biochemistry and immune responses in broiler chicks.

    Science.gov (United States)

    Toghyani, Majid; Mosavi, Seyed Kazem; Modaresi, Mehrdad; Landy, Nasir

    2015-12-01

    This experiment was conducted to evaluate the effect of milk or molasses kefir as a probiotic on growth performance, carcass traits, serum biochemistry and humoral immune responses in broiler chickens. A total of 192 one-d-old as hatched broiler chicks (Ross 308) were randomly allotted to 4 treatments, each with 4 replicate pens of 12 chicks. The following treatments were applied: 1) a basal diet (C) and normal drinking water, 2) 2% milk kefir in drinking water, 3) 2% molasses kefir in drinking water, and 4) the diet C supplemented with commercial probiotic. At d 42, eight birds per treatment were killed for determination of carcass traits. Broilers at 28 days of age were bled for measuring antibody titers against Newcastle disease virus (NDV) and avian influenza virus (AIV), at 30 days of age for antibody titers against sheep red blood cell (SRBC), and at 42 days of age for biochemical analysis. Supplementing 2% milk kefir increased body weight of broilers at 28 and 42 days of age ( P   0.05) influenced. Broilers supplemented with molasses kefir, had a significantly lower concentration of serum total cholesterol, low density lipoprotein cholesterol and elevated high density lipoprotein cholesterol at 42 days of age ( P  < 0.05). In conclusion, the results indicated that inclusion of 2% milk kefir in drinking water would improve growth performance of broiler chickens.

  20. Evaluation of kefir as a potential probiotic on growth performance, serum biochemistry and immune responses in broiler chicks

    Directory of Open Access Journals (Sweden)

    Majid Toghyani

    2015-12-01

    Full Text Available This experiment was conducted to evaluate the effect of milk or molasses kefir as a probiotic on growth performance, carcass traits, serum biochemistry and humoral immune responses in broiler chickens. A total of 192 one-d-old as hatched broiler chicks (Ross 308 were randomly allotted to 4 treatments, each with 4 replicate pens of 12 chicks. The following treatments were applied: 1 a basal diet (C and normal drinking water, 2 2% milk kefir in drinking water, 3 2% molasses kefir in drinking water, and 4 the diet C supplemented with commercial probiotic. At d 42, eight birds per treatment were killed for determination of carcass traits. Broilers at 28 days of age were bled for measuring antibody titers against Newcastle disease virus (NDV and avian influenza virus (AIV, at 30 days of age for antibody titers against sheep red blood cell (SRBC, and at 42 days of age for biochemical analysis. Supplementing 2% milk kefir increased body weight of broilers at 28 and 42 days of age (P  0.05 influenced. Broilers supplemented with molasses kefir, had a significantly lower concentration of serum total cholesterol, low density lipoprotein cholesterol and elevated high density lipoprotein cholesterol at 42 days of age (P < 0.05. In conclusion, the results indicated that inclusion of 2% milk kefir in drinking water would improve growth performance of broiler chickens.

  1. Arginine and vitamin E improve the immune response after a Salmonella challenge in broiler chicks

    Science.gov (United States)

    Two experiments were conducted to evaluate the effects of arginine (ARG), vitamin E (VE), and mannanoligosaccharide (MOS) on the immune response and clearance of Salmonella in broiler chickens. In each experiment, chicks were randomly distributed into 4 groups: antibiotic-free diet (negative contro...

  2. Efficacy of adsorbents (bentonite and diatomaceous earth) and turmeric (Curcuma longa) in alleviating the toxic effects of aflatoxin in chicks.

    Science.gov (United States)

    Dos Anjos, F R; Ledoux, D R; Rottinghaus, G E; Chimonyo, M

    2015-01-01

    A study was conducted to determine the efficacy of bentonite clay (BC), diatomaceous earth (DE) and turmeric powder (TUM) in alleviating the toxic effects of aflatoxin B1 (AFB1). A total of 250 Ross-308 d-old male broiler chicks were assigned to 10 dietary treatments (5 replicates of 5 chicks) from hatch to d 21. Dietary treatments were: basal diet; basal diet plus AFB1 (2 mg) or BC (0.75%), or DE (0.75%), or TUM (200 mg/kg curcuminoids) and different combinations of AFB1, BC, DE and TUM. Feed intake (FI), body weight gain (BWG) and feed gain (FG) of the birds fed on BC or DE separately were not different from control birds. Birds fed on TUM only had similar FI and FG but lower BWG than control chicks. Aflatoxin B1 reduced FI, BWG and serum concentrations of glucose, albumin, total protein calcium, but increased FG and relative liver and kidney weights. Chicks fed on the combination of AFB1 and BC had similar FI and FG to control chicks. Chicks fed on the combination of DE and AFB1 had lower FI (23.1%) and BWG (28.6%) compared with control chicks. Chicks fed on the combination of TUM and AFB1 also had decreased FI (26.2 %) and BWG (31%) compared with control chicks. Chicks fed on the combination of AFB1, BC and TUM consumed significantly higher amounts of feed compared with chicks fed on only AF, but gained less when compared with control diet chicks. Chicks fed on the combination of AFB1, DE and TUM diet had poorer growth performance than those fed on AFB1 alone. None of the combination diets reduced the severity of liver lesions.

  3. Effects of various stocking density on productive performance and some physiological traits of broiler chicks

    OpenAIRE

    Ihsan T. Tayeb,; Siamand Nizar Hassan,; Merkhan M. Mustafa,; Shawkat Abdulrazaq M. Sadeq,; Gulizar Issa Ameen,; Asia Mohamed Hassan

    2011-01-01

    This experiment was carried out as a survey at the commercial poultry farm of broiler chickens in Duhok region namely Amedy, Akry and Sumail in order to study the effect of stocking density on the chick performance and some physiological traits. Chicks were divided into three stocking densities namely 8.66, 10.41 and 13.36 birds/m². The results obtained are summarized as follows: Live body weights and feed conversion ratio at 7 weeks of age were non significant differences between different s...

  4. Influence of gamma radiation on productivity parameters of chicken fed mycotoxin-contaminated corn

    International Nuclear Information System (INIS)

    Simas, Monica M.S.; Albuquerque, Ricardo; Oliveira, Carlos A.; Rottinghaus, George E.; Correa, Benedito

    2010-01-01

    The aim of this study was to evaluate productivity parameters and carcass yield of broiler chickens fed irradiated corn contaminated with mycotoxins. For this purpose, 180 one-day-old male chicks were divided into nine treatments and fed for 42 days. The results indicated that irradiation of corn with 5 kGy improved the productivity parameters studied. Therefore, gamma radiation may become an alternative for the control of the deleterious effects of mycotoxins on broiler chickens, which cause marked economic losses for rural producers.

  5. Influence of gamma radiation on productivity parameters of chicken fed mycotoxin-contaminated corn

    Energy Technology Data Exchange (ETDEWEB)

    Simas, Monica M.S., E-mail: monicamssimas@yahoo.com.b [Microbiology Department, Biomedical Sciences Institute, University of Sao Paulo, Av. Prof. Lineu Prestes, 1374, Sao Paulo 05508-900 (Brazil); Albuquerque, Ricardo, E-mail: ricalbuq@usp.b [Nutrition and Animal Production Department, College of Veterinary Medicine, University of Sao Paulo, Av. Duque de Caxias Norte, 225 Pirassununga, Sao Paulo 13695-900 (Brazil); Oliveira, Carlos A., E-mail: carlosaf@usp.b [Food Science Department, College of Food Science, University of Sao Paulo, Av. Duque de Caxias Norte, 225, Pirassununga, Sao Paulo 13695-900 (Brazil); Rottinghaus, George E., E-mail: rottinghausg@missouri.ed [College of Veterinary Medicine, University of Missouri, 1600 East Rollins, Columbia, MO 65211 (United States); Correa, Benedito, E-mail: correabe@usp.b [Microbiology Department, Biomedical Sciences Institute, University of Sao Paulo, Av. Prof. Lineu Prestes, 1374, Sao Paulo 05508-900 (Brazil)

    2010-10-15

    The aim of this study was to evaluate productivity parameters and carcass yield of broiler chickens fed irradiated corn contaminated with mycotoxins. For this purpose, 180 one-day-old male chicks were divided into nine treatments and fed for 42 days. The results indicated that irradiation of corn with 5 kGy improved the productivity parameters studied. Therefore, gamma radiation may become an alternative for the control of the deleterious effects of mycotoxins on broiler chickens, which cause marked economic losses for rural producers.

  6. Effects of egg storage on hatchability, chick quality, performance and immunocompetence parameters of broiler chickens.

    Science.gov (United States)

    Goliomytis, Michael; Tsipouzian, Theofania; Hager-Theodorides, Ariadne L

    2015-09-01

    Pre-incubation egg storage is a necessity for the poultry industry. This study evaluated the effects of pre-incubation storage length of broiler eggs on hatchability, 1-day-old chick quality, subsequent performance, and immunocompetence. To this end, a total of 360 hatching eggs were stored for 4, 12, or 16 d prior to incubation. Hatchability and chick quality were assessed at hatch, and growth performance and immunocompetence parameters were assessed during a 35 d rearing period. Hatchability of set and fertile eggs, and embryonic mortality, were not affected by egg storage. On the contrary, 1-day-old chick BW and length were linearly negatively correlated with egg storage length (P-linearegg weight prior to setting was unaffected, and corrected chick length was positively affected by storage length. One-day-old chick Tona score, navel quality, and post-hatch growth performance (BW at 7 and 35 d, cumulative feed intake, and feed conversion ratio at 35 d) were unaffected by egg storage (P, P-linear>0.05). Lymphoid organ weights at 2 and 35 d, the titre of maternal anti-NDV antibodies, most of the thymocyte subpopulations defined by CD3, CD4, and CD8 cell surface expression in the thymus of 2-d-old chicks, cellular responses to the PHA skin test, humoral responses to primary SRBC, and NDV immunizations were also not influenced by length of storage (P, P-linear>0.05). On the contrary, the length of egg storage was found to negatively influence the abundance of CD3+CD4-CD8- thymocytes that represent the majority of γδ-T cells in the thymus of 2-day-old chicks, as well as the humoral response to booster NDV immunization of the birds. In brief, pre-incubation storage of broiler hatching eggs for up to 16 d did not affect most developmental and growth parameters investigated, except for BW and length at hatch. Egg storage was found to suppress some aspects of the immunocompetence of the birds, particularly aspects of acquired immunity. © 2015 Poultry Science

  7. Influence of a direct-fed microbial and xylanase enzyme on the dietary energy uptake efficiency and performance of broiler chickens.

    Science.gov (United States)

    Murugesan, Ganapathi Raj; Persia, Michael E

    2015-09-01

    Efficacy of a multi-strain direct-fed microbial product (PoultryStar(®) ME; PS) and a xylanase enzyme product on the dietary energy utilization efficiency and resulting performance in broiler chickens was evaluated. Apart from performance parameters, cecal and serum metabolites and activities of hepatic enzymes involved in energy metabolism were also determined. Ross 308 chicks were fed one of four experimental diets [control (CON), CON + PS, CON + xylanase and CON + PS + xylanase] using a 2 × 2 factorial arrangement from 1-21 days of age. Cecal proportions of propionate and butyrate, as well as total short-chain fatty acid concentration were increased (P energy uptake and hepatic energy retention. The combination additively increased the FCR, suggesting involvement of synergistic modes of actions. © 2014 Society of Chemical Industry.

  8. Behavioral changes and feathering score in heat stressed broiler chickens fed diets containing different levels of propolis

    Science.gov (United States)

    This experiment was conducted to evaluate the effects of dietary supplementation of green Brazilian propolis on behavioral patterns and feather condition of heat stressed broiler chickens. Five hundred and four (504) male Ross 708 broiler chicks at 15-day old were randomly allotted to six dietary tr...

  9. The Impact of in ovo Injection of Silver Nanoparticles, Thyme and Savory Extracts in Broiler Breeder eggs on Growth Performance, Lymphoid-Organ Weights, and Blood and Immune Parameters of Broiler Chicks

    Directory of Open Access Journals (Sweden)

    Saki AA

    2015-12-01

    Full Text Available This study was conducted to evaluate the long-term effects of in ovo injection of nano-silver, thyme and savory extracts in broiler breeder eggs on growth performance, lymphoid organ weights, immune and blood parameters and bone mineral concentration of broiler chicks. A total of 560 fertilized broiler breeder eggs were divided into 7 groups. On d 5 of incubation, eggs were treated with the following groups. Group 1 (control, no injection; group 2 (placebo, 1 mL of 0.9% NaCl; group 3 and 4 (placebo + 30 and 45 mg of nano-silver, respectively; group 5 and 6 (placebo + 75 and 100 mg of thyme, respectively and group 7 (placebo + 75 mg of savory. After hatch, chickens were fed a corn-soybean meal diet under the controlled conditions and slaughtered at 14 and 21 d of age for sample collection and analysis. The lymphoid-organ weights and growth rate were not affected by dietary treatments at 14 and 21 d of age. The results also showed that nano-silver injected into broiler breeder eggs during incubation improved the bone mineral concentration and cell-mediated immunity at 14 and 21 d of age, respectively. Humoral immunity was improved by thyme and savory extracts (P < 0.05. Overall, the effect of in ovo injection of nano-silver, thyme and savory extracts during embryonic development is a potential means to improve immune activities of broiler chickens, while does not have any detrimental effect on embryo hatchability.

  10. Effects of Onion Extracts on Growth Performance, Carcass Characteristics and Blood Profiles of White Mini Broilers

    Directory of Open Access Journals (Sweden)

    B. K. An

    2015-02-01

    Full Text Available This experiment was carried out to investigate effects of onion extract on growth performance, meat quality and blood profiles of White mini broilers. Total of 600 one-d-old male White mini broiler chicks were divided into four groups and fed control diets (non-medicated commercial diet or antibiotics medicated or experimental diets (non-medicated diets containing 0.3% or 0.5% onion extract for 5 wks. The final body weight (BW and weight gain of the group fed non-medicated control diet were lower than those of medicated control group (p<0.01. The chicks fed diet with 0.3% or 0.5% onion extract showed a similar BW to that of medicated control group. The relative weight of various organs, such as liver, spleen, bursa of Fabricius, abdominal fat, and the activities of serum enzymes were not affected by dietary treatments. There were no significant differences in meat color among groups. Whereas, groups fed diets containing onion extract had slightly lower cooking loss and higher shear force value, but not significantly. The concentrations of serum free cholesterol and triacylglycerol in groups fed diet containing onion extract were significantly decreased compared with those of controls (p<0.01. In conclusion, the onion extracts exerted a growth-promoting effect when added in White mini broiler diets, reflecting potential alternative substances to replace antibiotics.

  11. Effect of selenium deficiency on 75Se and 45Ca metabolism in chicks

    International Nuclear Information System (INIS)

    Edwardly, J.S.

    1981-01-01

    Two hundred, 1 day old broiler chicks were used to study the effect of selenium deficiency on 75 Se and 45 Ca metabolism in chicks. The chicks were randomly divided into five groups. One group was fed a purified basal diet low in selenium ( 75 Se or 45 Ca. Levels of radioisotopes in blood and tissue, as well as total body excretion of radioactivity were determined. Whole body retention of 75 Se was significantly higher (p 45 Ca was significantly higher in selenium deficient chicks than in supplemented animals. Total Ca concentration in muscle was also higher in this group. Studies of red cell uptake of 75 Se demonstrated a clear difference between Se repleted and selenium deficient groups. (author)

  12. Comparative effects of using black seed (Nigella sativa), cumin seed (Cuminum cyminum), probiotic or prebiotic on growth performance, blood haematology and serum biochemistry of broiler chicks.

    Science.gov (United States)

    Alimohamadi, K; Taherpour, K; Ghasemi, H A; Fatahnia, F

    2014-06-01

    A 42-day trial was conducted to compare the effects of the following seven experimental diets, which varied in black seed, cumin seed, probiotic or prebiotic concentrations, on the broiler chicks: control (no additives), diet BS1 (4 g/kg black seed), diet BS2 (8 g/kg black seed), diet CS1 (4 g/kg cumin seed), diet CS2 (8 g/kg cumin seed), diet Pro (1 g/kg probiotic Primalac(®)) and diet Pre (2 g/kg prebiotic Fermacto(®)). A total of 420 1-day-old male broiler chicks, initially weighing an average of 43 g, were distributed into 28 floor pens at a stocking density of 15 birds per pen. At 28 day of age, the body weight in the birds fed diets BS2, CS2 and Pro was significantly higher than in the control group, but final body weight was not affected. Additionally, the birds fed diets BS2, Pro and Pre exhibited better feed conversion ratio than control birds from 0 to 42 day of age. Diets BS2, CS2 and Pro also statistically increased the relative weight of thymus and bursa of Fabricius, whereas only diet Pro decreased the abdominal fat percentage compared with control diet. Regarding the haematological parameters, feeding diet BS2 yielded a significant increase in red blood cell count, haemoglobin concentration and haematocrit percentage compared with control diet. Serum total cholesterol and low-density lipoprotein cholesterol levels in the birds fed diets BS2, Pro and Pre were also significantly lower than in the birds fed the control diet. Without exception, no diets affected feed intake, internal organs weights, carcass characteristics, antibody titres against Newcastle and influenza viruses and leucocyte subsets. In general, current study showed promising results regarding the use of spice additives as growth and health promoters, especially at higher levels of their incorporation in the diets, which were comparable to the probiotic- or prebiotic-containing diets. Journal of Animal Physiology and Animal Nutrition © 2013 Blackwell Verlag GmbH.

  13. Broiler performance, hatching egg, and age relationships of progeny from standard and dwarf broiler dams.

    Science.gov (United States)

    Tahir, M; Cervantes, H; Farmer, C W; Shim, M Y; Pesti, G M

    2011-06-01

    The relationship of egg and chick weights to the performance of broiler chickens from two 42-wk-old flocks (standard and dwarf dams) having male parents from the same genetic stock was investigated in this study. Fertility (91.7 vs. 94.7%) and hatchability (95.2 vs. 96.3%) were not significantly (P > 0.10) different for eggs from standard and dwarf dams, respectively. Egg weight contributed significantly to the variation in BW [BW = β(0) + β(i) (egg weight) + β(i) (dam) + β(i) (sex)]. Body weight as a function of chick weight was not significant. However, chick weight was significant when included in a model with egg weight, suggesting that significant differences in BW at 50 d could be attributed to both egg and chick weights. The negative coefficient for chick weight indicated that between the 2 broilers of the same egg weight, the one with the greater chick weight would have the smaller 50-d BW. Chick weight was a linear function of egg weight. Similarly, the effect of egg or chick weight on broiler BW at 35 or 50 d was best represented by a single linear function. Dam genotype did not contribute significantly to variation in 50-d BW after variation attributable to egg weight was removed from the model. Differences in BW attributable to egg weight increased with broiler age. The coefficients of egg weight and chick weight showed that the differences in BW per gram of egg were 1.43, 3.06, 6.24, and 7.61 g and those per gram of chick were 1.87, 3.99, 8.14, and 9.93 g, respectively, at 7, 21, 35, and 50 d. Body weight increased by 0.1563 times egg weight (and 0.2092 times chick weight) with each additional day of age for both sexes and genotypes. Clearly, both egg and chick weights are important for modeling or predicting market-age broiler BW and economic returns. The relatively small relationship between BW and egg weight demonstrates that genetic selection over the past 3 decades has decreased the influence of egg weight on broiler growth. The present dwarf

  14. Effects of estrogen on very low-density lipoprotein triglyceride metabolism in fed and fasted chicks

    International Nuclear Information System (INIS)

    Park, J.R.

    1988-01-01

    A single injection of estrogen into growing chicks resulted in a marked elevation in plasma triglyceride (TG) followed by phospholipid (PL) and cholesterol (CH) in both fed and fasted chicks. Estrogen caused a development of massive fatty liver in fed chicks. Hepatic malic enzyme and glucose-6-phosphate dehydrogenase activities also increased significantly in fed chicks and, to a small extent, in fasted chicks. Very low density lipoproteins (VLDL) were barely detectable in the fasted control plasma. However, the VLDL concentration increased markedly upon estrogen injection, becoming the most prevalent lipoprotein in the plasma. The administration of estrogen resulted in an increase in oleic acid and a decrease in linoleic acid content except in the cholesteryl ester of VLDL and LDL. VLDL of estrogenized birds had β-mobility on agarose gel electrophoresis, and they eluted in two peaks on agarose gel filtration chromatography. Both peaks on gel filtration exhibited the same β-mobility on agarose gel electrophoresis. Nevertheless, the apoprotein composition of these two peaks were substantially different from each other; apo B was not present in the first peak VLDL. VLDL-TG kinetic studies conducted in vivo, using 14 C-TG-VLDL prepared endogenously from control and estrogenized chicks revealed that VLDL-TG produced from the former had a higher fractional catabolic rate (FCR) than VLDL-TG from the latter

  15. Growth Performance, Carcass Characteristics and Serum Biochemistry of Broiler Chicken Fed Graded Levels of Sun-Dried Irish Potato Peel Meal

    Directory of Open Access Journals (Sweden)

    Wafar James Raphael

    2017-06-01

    Full Text Available An experiment was conducted to evaluate the effect of substituting different levels of sun-dried Irish potato peel meal (SPPM for maize in diets of broiler chickens. Five diets in which SPPM was replaced with 0 (0SPPM, 25 (25SPPM, 50 (50SPPM, 75 (75SPPM and 100% (100SPPM of maize in starter and finisher diets for a 8 week period using broiler chickens were examined. A total of 300 two weeks old broilers chicks Anak 2000 were randomly allotted to five experimental diets with six pen per treatment and 10 birds chicks per pen. Body weight and feed intake of broilers, and feed conversion ratio were determined at the beginning and 56th day of the experiment respectively. On day 56, four broilers from each pen were selected and slaughtered to determine some carcass characteristics and serum biochemistry. The result of growth performance showed that 100SPPM decreased daily weight gain of chickens when compared to other SPPM levels (P

  16. Effects of Defatting Combined or not to Heating of Jatropha curcas Kernel Meal on Feed Intake and Growth Performance in Broiler Chickens and Chicks in Senegal

    Directory of Open Access Journals (Sweden)

    Nesseim, TDT.

    2017-01-01

    Full Text Available Jatropha curcas is a tropical plant belonging to the Euphorbiaceae family whose cultivation has been largely promoted in recent years for the production of biofuels. The kernel of the seed contains approximately 55% lipid in dry matter and the meal obtained could be an exceptional source of proteins for family poultry farming, after treatments to remove toxic and anti-nutritional compounds. The ingestion and the growth performance of J. curcas kernel meal (JKM, obtained after partial physico-chemical de-oiling combined or not with heating was evaluated in broiler chickens and chicks. Sixty unsexed broiler chickens, 30 day-old, divided into three groups as well as twenty broiler chicks, 1 day-old, divided into two groups were used in two experiments. In experiment 1, jatropha kernel was de-oiled and incorporated into a control fattening feed at 40 and 80g/kg (diets 4JKM1 and 8JM1. In experiment 2, jatropha kernel meal obtained in experiment 1 was heat treated and incorporated into a growing diet at 80g/kg (diet 8JKM2. Daily dietary intakes as well as weight gain of the animals were affected by the incorporation of jatropha kernel meal in the ration. In experiment 1, average daily feed intake (ADFI1 of 139.2, 55.2 and 23.4g/day/animal and also average daily weight gain (ADWG1 of 61.9, 18.5 and -7.7g/animal were obtained respectively for the groups fed with diets 0JKM1, 4JKM1 and 8JKM1. In experiment 2, Average daily feed intake (ADFI2 of 18.7 and 3.1g/day/animal and also average daily weight gain (ADWG2 of 7.1 and 1.9g/animal were obtained respectively for the groups fed with diets 0JKM2 and 8JKM2. In both experiment, feed conversion ratio (FCR was also affected by the dietary treatments and the overall mortality rate showed an increase according to levels of jatropha kernel meal in diet.

  17. Effects of Xylanase Supplementation on Growth Performance, Nutrient Digestibility and Non-starch Polysaccharide Degradation in Different Sections of the Gastrointestinal Tract of Broilers Fed Wheat-based Diets

    Directory of Open Access Journals (Sweden)

    L. Zhang

    2014-06-01

    Full Text Available This experiment was performed to investigate the effects of exogenous xylanase supplementation on performance, nutrient digestibility and the degradation of non-starch polysaccharides (NSP in different sections of the gastrointestinal tract (GIT of broilers fed wheat-based diets. A total of 120 7-day-old Arbor Acres broiler chicks were randomly allotted to two wheat-based experimental diets supplemented with 0 or 1.0 g/kg xylanase. Each treatment was composed of 6 replicates with 10 birds each. Diets were given to the birds from 7 to 21 days of age. The results showed that xylanase supplementation did not affect feed intake, but increased body weight gain of broiler at 21 day of age by 5.8% (pjejunum>duodenum>>gizzard> caecum. The supplementation of xylanse increased ileal isomaltriose concentration (p<0.05, but did not affect the concentrations of isomaltose, panose and 1-kestose in the digesta of all GIT sections. These results suggest that supplementation of xylanase to wheat-based diets cuts the arabinoxylan backbone into small fragments (mainly arabinose and xylose in the ileum, jejunum and duodenum, and enhances digestibilites of nutrients by decreasing digesta viscosity. The release of arabinose and xylose in the small intestine may also be the important contributors to the growth-promoting effect of xylanase in broilers fed wheat-based diets.

  18. Immunomodulation in gut-associated lymphoid tissue of neonatal chicks by immunobiotic diets.

    Science.gov (United States)

    Sato, K; Takahashi, K; Tohno, M; Miura, Y; Kamada, T; Ikegami, S; Kitazawa, H

    2009-12-01

    Developmental changes in immunocompetent cells of the gut during the first week posthatch were determined in broiler chicks fed immunobiotic lactic acid bacteria in the form of Lactobacillus jensenii TL2937-, Lactobacillus gasseri JCM1131(T)-, Lactobacillus delbrueckii ssp. bulgaricus NIAIB6-, or L. gasseri TL2919-supplemented diets. The relative weights of spleen and bursa of Fabricius in chicks fed the immunobiotic diets were slightly higher than the control valued at 1 and 3 d of age, with the exception of spleen weight in the L. gasseri JCM1131(T) at 3 d of age, the bursa of Fabricius weight in the L. gasseri JCM1131(T) at 1 and 3 d of age, and bursa of Fabricius weight in the L. gasseri TL2919 group at 1 d of age. There were no significant differences in body and liver weights among the treatments. When chicks were fed the L. jensenii TL2937- or L. gasseri TL2919-supplemented diets, expression of T cell-related mRNA [cluster of differentiation 3 (CD3), interleukin-2 (IL-2), and interferon-gamma (IFN-gamma)] in the foregut was significantly higher than that of control chicks at 3 or 7 d of age. Expression levels of toll-like receptor (TLR) mRNA tended to increase in the foregut of chicks fed the immunobiotic diets, except for the L. delbrueckii ssp. bulgaricus NIAIB6, compared with expression levels in control chicks. The Bu-1 mRNA expression levels in the bursa of Fabricius were not affected by the supplementations with immunobiotic lactic acid bacteria. These results show that immunobiotics, particularly L. gasseri TL2919, might be useful as immunomodulators to stimulate the gut-associated immune system in neonatal chicks, and thereby protect them from disease without decreasing growth performance as a possible substitution of antibiotics.

  19. Some blood indices in finisher broiler chickens fed cocoa pod husk ...

    African Journals Online (AJOL)

    The dietary effects of pre-treated cocoa pod husk (CPH) on some blood indices of 21-day old COBB-500 finisher broiler chicks were evaluated in a 35-day experiment with a completely randomized design. The birds were allotted to seven treatments (diets) having 0-g kg-1, 100- g kg-1, 200-g kg-1 and 300-g kg-1 of either ...

  20. Performance of chicken broilers fed with diets substituted with ...

    African Journals Online (AJOL)

    Performance of chicken broilers fed with diets substituted with mulberry leaf powder. Carlina Freddie Simol, Andrew Alek Tuen, Humrawali Hazid Ahmad Khan, John Keen Chubo, Patricia Jie Hung King, Kian Huat Ong ...

  1. A preliminary study on growth response of broiler finishers fed ...

    African Journals Online (AJOL)

    A preliminary study on growth response of broiler finishers fed processed mottle Mucuna beans ( Mucuna pruriens var. utilis ) ... They were fed diets (20% CP, 13 MJME/kg) incorporating 0%, 5% and 10% processed mottle “Mucuna” beans. A completely randomized design was used. Feed and water were supplied and ...

  2. Effect of Different Levels of Molasses Fed Through Drinking Water ...

    African Journals Online (AJOL)

    An experiment was conducted to evaluate the effect of molasses fed through drinking water on growth and economic performance of broiler chickens. One hundred and sixty unsexed day old chicks of Anak strain were used. They were divided into four treatment groups with each group having four replicates of ten birds per ...

  3. Effect of dietary vitamin A and Nigella sativa on the performance of broiler chicks

    International Nuclear Information System (INIS)

    Sideeg, Rasha Mohamed

    2000-02-01

    A study was conducted to assess the effect of feeding different added levels of vitamin A and Nigella sativa seeds on broiler performance, blood chemistry and carcass characteristics. One- hundred forty four, one-day old unsexed (Lohman) broiler chicks were divided randomly into eight groups, each represented a treatment (18 birds/treatment), with 2 replicates for each treatment 2x4 factorial arrangement in a completely randomised design was used. The experimental basal rations were formulated to meet requirement for essential nutrients for broiler chicks according to NCR (1984) recommendation. Four graded levels of added vitamin A (0,3000,4500 and 9000 IU/kg) and two levels of Nigella sativa (0, 0.25%) were used. The experiment lasted for eight weeks. Body weight, weight gain, feed intake and feed conversion ratio were determined. In addition blood chemistry, absolute weight of internal organs and carcass characteristics were measured. The added level 3000 IU of vitamin A significantly increased weight gain (P≤ 0.01) decreased feed conversion ratio (P≤ 0.01) and increased muscle: bone ratio (P≤ 0.05). The level 0.25% of Nigella sativa with no added vitamin A significantly (P ≤ 0.01) increased body weight and weight gain. High level of added vitamin A decreased absolute weight of liver (P≤ 0.01), and increased fat content of muscles (P≤ 0.01) and abdominal fat (P≤ 0.01). Vitamin A and Nigella sativa significantly affect serum Ca (which was determined according to the calorimetric method), P, Zn , and alkaline phosphates(alk.ase) and cholesterol (P≤ 0.05) which was determined according to the Enzymatic Calometric Test . Addition of 0.25% Nigella sativa significantly affect serum cholestrol (P ≤0.01). The treatments had no significant affect on serum glucose and ash content of meat. Significant interaction between vitamin A and Nigella sativa were observed on body weight gain, feed conversion ratio, feed intake (P≤ 0.01, P≤0.05, and P≤0

  4. Effect of dietary vitamin A and Nigella sativa on the performance of broiler chicks

    Energy Technology Data Exchange (ETDEWEB)

    Sideeg, Rasha Mohamed [Faculty of Animal Production, University of Khartoum, Khartoum (Sudan)

    2000-02-01

    A study was conducted to assess the effect of feeding different added levels of vitamin A and Nigella sativa seeds on broiler performance, blood chemistry and carcass characteristics. One- hundred forty four, one-day old unsexed (Lohman) broiler chicks were divided randomly into eight groups, each represented a treatment (18 birds/treatment), with 2 replicates for each treatment 2x4 factorial arrangement in a completely randomised design was used. The experimental basal rations were formulated to meet requirement for essential nutrients for broiler chicks according to NCR (1984) recommendation. Four graded levels of added vitamin A (0,3000,4500 and 9000 IU/kg) and two levels of Nigella sativa (0, 0.25%) were used. The experiment lasted for eight weeks. Body weight, weight gain, feed intake and feed conversion ratio were determined. In addition blood chemistry, absolute weight of internal organs and carcass characteristics were measured. The added level 3000 IU of vitamin A significantly increased weight gain (P{<=} 0.01) decreased feed conversion ratio (P{<=} 0.01) and increased muscle: bone ratio (P{<=} 0.05). The level 0.25% of Nigella sativa with no added vitamin A significantly (P {<=} 0.01) increased body weight and weight gain. High level of added vitamin A decreased absolute weight of liver (P{<=} 0.01), and increased fat content of muscles (P{<=} 0.01) and abdominal fat (P{<=} 0.01). Vitamin A and Nigella sativa significantly affect serum Ca (which was determined according to the calorimetric method), P, Zn , and alkaline phosphates(alk.ase) and cholesterol (P{<=} 0.05) which was determined according to the Enzymatic Calometric Test . Addition of 0.25% Nigella sativa significantly affect serum cholestrol (P {<=}0.01). The treatments had no significant affect on serum glucose and ash content of meat. Significant interaction between vitamin A and Nigella sativa were observed on body weight gain, feed conversion ratio, feed intake (P{<=} 0.01, P{<=}0.05, and P

  5. Protection of Broiler Chicks Housed with Immunized Cohorts Against Infection with Eimeria maxima and E. acervulina.

    Science.gov (United States)

    Fetterer, Raymond H; Barfield, Ruth C; Jenkins, Mark C

    2015-03-01

    The use of live oocyst vaccines is becoming increasingly important in the control of avian coccidiosis in broilers. Knowledge of the mechanisms employed when chicks uptake oocysts and become immune is important for optimizing delivery of live vaccines. The current study tests the hypothesis that chicks not initially immunized may ingest oocysts by contact with litter containing oocysts shed by immunized cohorts. In Experiment 1, day-old broiler chicks were housed in pens containing clean litter. In Trial 1, 100% of chicks in some pens were immunized with 2.5 X 10(3) Eimeria acervulina oocysts while in other pens only 75% of chicks were immunized and remaining cohorts within the pens were not immunized. Other pens contained chicks that served as nonimmunized nonchallenged controls or nonimmunized challenged controls (NIC). On day 21, birds were given a homologous challenge of 6 X 10(5) oocysts. A second identical trial was conducted, except birds were immunized with 500 Eimeria maxima oocysts and were challenged with 3 X 10(3) E. maxima oocysts. In Experiment 2, 100% of chicks in some pens were immunized with 500 E. acervulina oocysts while in other pens either 75% or 50% of the birds were immunized. On day 14, birds were challenged with 1 X 10(6) oocysts. Trial 2 was identical to Trial 1 except that birds were immunized with 100 E. maxima oocysts and challenged with 1 X 10(6) oocysts. For all experiments weight gain, feed conversion ratio (FCR), plasma carotenoids, and litter oocyst counts were measured. In Experiment 1, the level of protection in groups containing 25% nonimmunized cohorts, as measured by weight gain, carotenoid level, FCR, and oocyst litter counts, was identical to groups containing 100% immunized chicks. In Experiment 2, pens where 50% or 75% of birds were immunized with either E. maxima or E. acervulina were not well protected from decreases in weight gain and plasma carotenoids nor from increases in litter oocyst counts following a challenge

  6. Body weight and carcass characteristics of broilers fed different ...

    African Journals Online (AJOL)

    Body weight and carcass characteristics of broilers fed different mixtures of ... Journal of Agriculture, Forestry and the Social Sciences ... The experimental diets (starter and finisher diets) were offered to the respective bids with water ad libitum.

  7. Performance characteristics of broilers fed graded levels of Moringa oleifera leaf meal

    Directory of Open Access Journals (Sweden)

    Ayo-Ajasa, O.Y.

    2016-06-01

    Full Text Available Cost of conventional protein sources is on the increase recently; hence, there is the need for cheaper alternative sources that will not compromise the performance characteristics of broiler birds taking into consideration the cost of production. Moringa leaf meal has been reported to increase the performance of broiler birds due to its rich protein content. Two hundred day-old broiler chicks were used to assess the effects of partial replacement of soya bean meal with Moringa (Moringa oleifera leaf meal on broiler chickens in an 8-wk feeding trial. The birds were randomly assigned in equal numbers into five dietary treatments: 0, 5, 10, 15, and 20 % Moringa leaf meal (MOLM. Each treatment was replicated four times with 10 birds per replicate. The results showed that final weight, weight gain, daily weight gain, total feed intake, daily feed intake and feed conversion ratio were significantly (p0.05 effect on primal cut parts and relative organ weight. From the results of this study, replacement of soybean meal with MOLM up to 20% did not have any adverse effect on growth performance and carcass traits of broiler chickens.

  8. MSTN, mTOR and FoxO4 are involved in the enhancement of breast muscle growth by methionine in broilers with lower hatching weight.

    Directory of Open Access Journals (Sweden)

    Chao Wen

    Full Text Available Broilers with lower hatching weight (HW present poorer performance than those with high HW, but there is limited research on the growth regulation of broilers with lower HW. The objective of this study was to investigate the effect of dietary methionine (Met levels on the growth performance and breast muscle yield of broilers with different HW and underlying mechanisms. A total of 192 one-day-old Arbor Acres broiler chicks with different HW (heavy: 48.3±0.1 g, and light: 41.7±0.1 g were allocated to a 2×2 factorial arrangement with 6 replicates of 8 chicks per replicate cage. Control starter (1-21 d and finisher (22-42 d diets were formulated to contain 0.50% and 0.43% Met, respectively. Corresponding values for a high Met treatment were 0.60% and 0.53%. Light chicks had lower body weight gain (BWG and breast muscle yield than heavy chicks when the broilers were fed the control diets. High Met diets improved BWG, gain to feed ratio and breast muscle yield in light but not heavy chicks. Decreased DNA content and increased RNA/DNA and protein/DNA ratios in breast muscle were induced by high Met diets only in light chicks. MSTN mRNA level was decreased by high Met diets only in light chicks, and this decrease was accompanied by a significant increase in MSTN gene exon 1 methylation. In addition, high Met diets increased mTOR phosphorylation, but decreased FoxO4 phosphorylation in breast muscle of light chicks. In conclusion, the BWG and breast muscle yield of light chicks were improved by increasing dietary Met levels probably through alterations of MSTN transcription and phosphorylation of mTOR and FoxO4.

  9. By-product of Tropical Vermicelli Waste as a Novel Alternative Feedstuff in Broiler Diets

    Directory of Open Access Journals (Sweden)

    P. Rungcharoen

    2013-12-01

    Full Text Available Two experiments were conducted to determine physical and chemical properties of vermicelli waste (VW and effect of VW inclusion levels on growth performance of broilers. In experiment 1, VW samples were randomly collected from vermicelli industry in Thailand to analyze nutritional composition. Vermicelli waste contained 9.96% moisture, 12.06% CP, 32.30% crude fiber (CF, and 0.57% ether extract (EE, as DM basis. The ratio of insoluble:soluble non-starch polysaccharide (NSP was 43.4:8.9. A total of 120 chicks (6 pens per treatment and 10 chicks per pen were fed a corn-soybean meal-based diet or 20% VW substituted diet to determine the apparent metabolizable energy corrected for nitrogen retention (AMEn of VW. The AMEn of VW was 1,844.7±130.71 kcal/kg. In experiment 2, a total of 1,200 chicks were randomly allotted to 1 of 4 dietary treatments for 42-d growth assay. There were 300 chicks with 6 pens per treatment and 50 chicks per pen. The dietary treatments contained 0%, 5%, 10%, or 15% VW, respectively. All diets were formulated to be isocaloric and isonitrogenous. From 0 to 18 d of age chicks fed VW diets had higher (p<0.001 feed conversion ratio (FCR compared with those fed the control diet. No difference was observed during grower and finisher phase (19 to 42 d. Chicks fed VW diets had lower relative weight of abdominal fat (p<0.001 but higher relative weight of gizzard (p<0.05 than those of chicks fed the control diet. Increasing VW inclusion levels increased ileal digesta viscosity (p<0.05 and intestinal villus height of chicks (p< 0.001. For apparent total tract digestibility assay, there were 4 metabolic cages of 6 chicks that were fed experimental treatment diets (the same as in the growth assay in a 10-d total excreta collection. Increasing VW inclusion levels linearly decreased (p<0.05 apparent total tract digestibility of DM and CF.

  10. Effects of polymannuronate on performance, antioxidant capacity, immune status, cecal microflora, and volatile fatty acids in broiler chickens.

    Science.gov (United States)

    Zhu, Wenhui; Li, Defa; Wang, Jianhong; Wu, Hui; Xia, Xuan; Bi, Wanghua; Guan, Huashi; Zhang, Liying

    2015-03-01

    The aim of this study was to assess the effects of purified polymannuronate (PM) obtained from marine brown algae on the performance, antioxidant capacity, immune status, and cecal fermentation profile of broiler chickens. In a 42 d experiment, 540 (average BW 43.77±1.29 g) 1-d-old Arbor Acres male broilers were randomly divided into 5 treatments with 6 replicates of 18 chicks and fed a corn and soybean meal (SBM)-based diet supplemented with 0, 1, 2, 3, or 4 g/kg polymannuronate. Adding polymannuronate to the broiler chickens' diets resulted in a significantly increased ADG and improved feed conversion compared with the control treatment. From d 1 to 42, the ADG of broilers fed 1, 2, 3, or 4 g/kg of polymannuronate was increased by 2.58, 4.33, 4.20, and 3.47%, respectively. Furthermore, parameters related to immune status, antioxidant capacity, and composition of the cecal microflora in broiler chickens fed the polymannuronate-containing diets were altered compared with broiler chickens fed a diet without polymannuronate. Supplementation with polymannuronate significantly increased the concentrations of lactic acid and acetic acid in the cecum compared with the control group. The results indicate that polymannuronate has the potential to improve broiler chicken immune status, antioxidant capacity, and performance. © 2015 Poultry Science Association Inc.

  11. Performance and economic analysis of broilers fed diets containing acerola meal in replacement of corn

    Directory of Open Access Journals (Sweden)

    Leonardo Henrique Zanetti

    2014-12-01

    Full Text Available It was aimed with this research to evaluate the performance of broiler chickens fed diets containing increasing levels of acerola meal in replacement of corn and analyze the economic viability of that production system. A total of 980 day-old male Cobb chicks were used, allotted in a completely randomized design, with four treatments - inclusion levels (0%, 5%, 10% and 15% of acerola meal in replacement of corn – and seven replications with 35 birds per experimental unit. The birds were weighed at 21 and 42 days of age to measure the performance data. In the period between 1 and 21 days of age, differences were observed in the feed intake and feed:gain only, in which the inclusion of acerola meal linearly decreased feed intake and improved feed:gain up to a level of 10.25%. For the total rearing period, the control treatment showed higher body weight. The feed:gain showed linear effect, worsening the results with increasing amounts of acerola meal in the diets. In the same period, there was no significant difference in feed intake and productive efficiency index. With regard to the economic analysis, it was found that the lowest final cost per broiler was observed in diets with 10% of acerola meal inclusion in replacement of corn.

  12. Performance of broiler chickens fed on Moringa oleifera leaf meal ...

    African Journals Online (AJOL)

    Performance of broiler chickens fed on Moringa oleifera leaf meal ... This exploratory study was conducted to investigate the effect of Moringa oleifera leaf meal ... ratio were evaluated for the individual replicate of each dietary treatment.

  13. Dietary inclusion of chromium to improve growth performance and immune-competence of broilers under heat stress

    Directory of Open Access Journals (Sweden)

    Tagwa M. Norain

    2013-11-01

    Full Text Available This study was conducted to investigate the effects of dietary supplementation with chromium chloride, CrCl3.6H2O (2mg kg–1 basal diet on the performance and immune response of broiler chickens under heat stress condition (25-43°C. A total of 80 one-day-old broiler chicks (Ross-308 were assigned to two treatment groups according to a completely randomized design. Each treatment consisted of four equal replicates, each contained ten chicks. Chicks were fed on basal diets supplemented with different concentrations of chromium (0 and 2 mg kg–1 CrCl3 from 1 to 35 days of age. Chromium supplementation as feed additives resulted in a slightly lower rectal temperature, and significantly (P<0.05 lower respiration rate for the broiler chickens received diet supplemented with chromium compared to the control (0 mg kg–1 CrCl3. Dietary chromium supplementation increased final body weight (BW at the end of the production period (5 weeks. Average weight gain was significantly (P<0.05 higher in chickens fed on chromium supplemented diet. Feed intake was not influenced by dietary chromium supplementation, however, the efficiency of feed conversion was improved (P<0.05 in chromium supplemented chickens. Furthermore, dressing percentage was significantly (P<0.05 higher in Cr-treated chickens compared to control chickens. Chromium supplementation significantly (P<0.05 improved the immune response to Newcastle Disease Virus vaccine (NDV. The present results suggest that dietary chromium supplementation provides a good nutritional management approach to ameliorate heat stress induced depression in production performance and immune response of broiler chickens. 

  14. Effects of feeding blends of grains naturally contaminated with Fusarium mycotoxins on production and metabolism in broilers.

    Science.gov (United States)

    Swamy, H V L N; Smith, T K; Cotter, P F; Boermans, H J; Sefton, A E

    2002-07-01

    Three hundred sixty, 1-d-old male broiler chicks were fed diets containing grains naturally contaminated with Fusarium mycotoxins for 56 d. The four diets included control (0.14 mg/kg deoxynivalenol, 18 mg/ kg fusaric acid, effect on serum albumin and y-glutamyltransferase activity. Blood hemoglobin and biliary IgA concentrations, however, responded in significant linear and quadratic fashions. Supplementation of E-GM counteracted most of the blood parameter alterations caused by the Fusarium mycotoxin-contaminated grains and reduced breast muscle redness. It was concluded that broiler chickens may be susceptible to Fusarium mycotoxicoses when naturally contaminated grains are fed containing a combination of mycotoxins.

  15. Evaluation of Immunodulatory effect of Stresroak Premix in Broiler Chick

    Directory of Open Access Journals (Sweden)

    M.M.Gatne

    2010-06-01

    Full Text Available The present study was aimed to evaluate the immunomodulatory efficacy of polyherbal formulation Stresroak along with effect on performance in broiler chicken. Four hundred and fifty healthy days old chicks were randomly divided into three groups (each group consisting of 150 chicks. Group A served as a negative control while group B served as positive control supplemented with levamisole, a known immunomodulator @ 15 mg/kg body weight through water for four consecutive days from third day onwards. Group C birds supplemented with Stresroak were administered @ 1 kg /tone of feed for consecutively for 11 days i.e. 5 days before and 5 days after vaccination. Parameters under study were performance traits viz, body weight gain, feed consumption, FCR, blood samples were collected at weekly interval for evaluation of various haematological, biochemical immunological and histopathological examination parameters. Results revealed that the antibody titre, total proteins and globulins were increased significantly in the birds receiving Stresroak as compared to untreated control group. Performance parameters were improved non-significantly as compared to untreated control (Group A. Histopathologically varying degrees of lympho-proliferative changes noted which reveals increase number of lymphocytes. It was concluded that Stresroak exhibits hematinic, hepato-protective and Immunomodulator properties [Vet. World 2010; 3(3.000: 122-125

  16. Performance, carcass yield, and meat quality of free-range broilers fed wet grain corn silage

    Directory of Open Access Journals (Sweden)

    ESPB Saldanha

    2006-06-01

    Full Text Available This study aimed at evaluating the effect of total replacement of dry corn by wet grain corn silage (WGCS in the feed of label broilers older than 28 days of age on performance, mortality, carcass, parts, breast meat and thighs meat yields, and meat quality. A mixed-sex flock of 448 ISA S 757-N (naked-neck ISA JA Label day-old chicks was randomly distributed in to randomized block experimental design with four treatments (T1 - with no WGCS; T2 - WGCS between 28 and 83 days; T3 - WGCS between 42 and 83 days; and T4 - WGCS between 63 and 83 days and four replicates of 28 birds each. Birds were raised under the same management and feeding conditions until 28 days of age, when they started to have free access to paddock with pasture (at least 3m²/bird and to be fed the experimental diets. Feed and water were offered ad libitum throughout the rearing period, which was divided in three stages: starter (1 to 28 days, grower (29 to 63 days, and finisher (64 to 83 days according to the feeding schedule. During the short periods of WGCS use (group T2 during grower stage and T4 during the finisher stage, performance and mortality results were similar as to those of the control group (T1. At the end of the experiment, it was observed that the extended use of WGCS (T2 and T3 determined a negative effect on feed conversion ratio. However, the best results of breast meat yield were observed with birds fed WGCS since 28 days (T2. It was concluded that WGCS can replace dry corn grain for short periods during the grower and finisher stages with no impairment of meat quality and yield in slow growth broilers.

  17. Effect of various fiber types and choice feeding of fiber on performance, gut development, humoral immunity, and fiber preference in broiler chicks.

    Science.gov (United States)

    Sadeghi, Amin; Toghyani, Majid; Gheisari, Abasali

    2015-11-01

    Two experiments were conducted to investigate the effect of fibrous materials with one single diet or by choice feeding on performance, intestinal morphology, immunity, and fiber preference in broiler chicks. In experiment 1, 240-day-old chicks (Ross 308) were assigned to one of 4 treatments, comprising 5 replicates per treatment in a completely randomized design. Dietary treatments included: a basal diet (control) or 30 g/kg sugar beet pulp (SBP), 30 g/kg rice hull (RH), or 30 g/kg equal combination of them (SBP/RH) added to the basal diet. Results showed SBP and SBP/RH impaired daily weight gain (DWG) in the growing period compared with control (P immunity. In addition, broilers had a tendency to use separate sources of fiber. © 2015 Poultry Science Association Inc.

  18. Multiple organ histopathological changes in broiler chickens fed on genetically modified organism.

    Science.gov (United States)

    Cîrnatu, Daniela; Jompan, A; Sin, Anca Ileana; Zugravu, Cornelia Aurelia

    2011-01-01

    Diet can influence the structural characteristics of internal organs. An experiment involving 130 meat broilers was conducted during 42 days (life term for a meat broiler) to study the effect of feed with protein from genetically modified soy. The 1-day-old birds were randomly allocated to five study groups, fed with soy, sunflower, wheat, fish flour, PC starter. In the diet of each group, an amount of protein from soy was replaced with genetically modified soy (I - 0%, II - 25%, III - 50%, IV - 75%, V - 100% protein from genetically modified soy). The level of protein in soy, either modified, or non-modified, was the same. Organs and carcass weights were measured at about 42 days of age of the birds and histopathology exams were performed during May-June 2009. No statistically significant differences were observed in mortality, growth performance variables or carcass and organ yields between broilers consuming diets produced with genetically modified soybean fractions and those consuming diets produced with near-isoline control soybean fractions. Inflammatory and degenerative liver lesions, muscle hypertrophy, hemorrhagic necrosis of bursa, kidney focal tubular necrosis, necrosis and superficial ulceration of bowel and pancreatic dystrophies were found in tissues from broilers fed on protein from genetically modified soy. Different types of lesions found in our study might be due to other causes (parasites, viral) superimposed but their presence exclusively in groups fed with modified soy raises some serious questions about the consequences of use of this type of feed.

  19. Advanced Oxidation Process sanitization of hatching eggs reduces Salmonella in broiler chicks.

    Science.gov (United States)

    Rehkopf, A C; Byrd, J A; Coufal, C D; Duong, T

    2017-10-01

    The microbial quality of eggs entering the hatchery is an important critical control point for biosecurity, pathogen reduction, and food safety programs in poultry production. Developing interventions to reduce Salmonella contamination of eggs is important to improving the microbial food safety of poultry and poultry products. The hydrogen peroxide (H2O2) and ultraviolet light (UV) Advanced Oxidation Process (AOP) has been previously demonstrated to be effective in reducing Salmonella on the surface of experimentally contaminated eggs. The objective of this study was to evaluate the effect of treating eggs with an egg-sanitizing apparatus using the H2O2/UV AOP on Salmonella contamination during incubation, hatching, and in broiler chicks during grow-out. Experimentally contaminated eggs were treated using the automated H2O2/UV AOP egg sanitizer and incubated for 21 d. AOP sanitization reduced Salmonella up to 7 log10 cfu egg-1 (P Salmonella positive eggs by up to 75% (P Salmonella-positive eggs during incubation. Additionally, Salmonella was recovered from more chicks hatched from untreated eggs than from eggs treated using the H2O2/UV AOP egg sanitizer (P Salmonella contamination on the surface of eggs using the H2O2/UV AOP egg sanitizer prior to incubation may reduce the gastrointestinal colonization of chicks by Salmonella. © 2017 Poultry Science Association Inc.

  20. An alternative to antibiotic-based drugs in feed for enhancing performance of broilers grown on Eimeria spp.-infected litter.

    Science.gov (United States)

    Stanley, V G; Gray, C; Daley, M; Krueger, W F; Sefton, A E

    2004-01-01

    Three trials were conducted to evaluate the effects of lasalocid, an anticoccidial feed additive (90.7 kg/ton); bacitracin, a growth-promoter (50 g/ton); and yeast culture residue (YCR) (1 kg/ton) on the performance of broiler chicks reared to 42 d of age on recycled litter. Recycled litter consisted of pine wood shavings containing droppings from chicks infected with 3 select strains of coccidia (Eimeria tenella, Eimeria maxima, and Eimeria acervulina). Response variables (BW, intestinal tract and litter coliform counts, cecal and liver relative weights, and litter moisture content) were recorded biweekly. Mean BW of chicks fed the diet supplemented with YCR was higher than that of the controls (P litter aged and moisture content increased. The mean intestinal coliform population from YCR-treated chicks was lower (P litter coliform counts increased with increased use of the litter. Cecal and liver relative weights calculated from the chicks in trial 3 showed that only the liver was significantly affected by treatments. YCR appeared to be a viable alternative to bacitracin and lasalocid medication in enhancing growth of broiler chicks reared on recycled litter.

  1. Effects of feeding grains naturally contaminated with Fusarium mycotoxins on performance and metabolism of broiler breeders.

    Science.gov (United States)

    Yegani, M; Smith, T K; Leeson, S; Boermans, H J

    2006-09-01

    A study was conducted to investigate the effects of feeding grains naturally contaminated with Fusarium mycotoxins on performance and metabolism of broiler breeders. Forty-two 26-wk-old broiler breeder hens and nine 26-wk-old roosters were fed the following diets: (1) control, (2) contaminated grains, and (3) contaminated grains + 0.2% polymeric glucomannan mycotoxin adsorbent (GMA) for 12 wk. The major contaminant was deoxynivalenol (12.6 mg/kg of feed), with lesser amounts of zearalenone and 15-acetyl-deoxynivalenol. Feed consumption and BW were not affected by diet. The feeding of contaminated grains did not significantly affect egg production. Decreased eggshell thickness was seen, however, at the end of wk 4, and dietary supplementation with GMA prevented this effect. There was no effect of diet on other egg parameters measured. There was a significant increase in early (1 to 7 d) embryonic mortality in eggs from birds fed contaminated grains at wk 4, but mid- (8 to 14 d) and late- (15 to 21 d) embryonic mortalities were not affected by diet. There were no differences in newly hatched chick weights or viability. The ratio of chick weight to egg weight was not affected by the feeding of contaminated grains. Weight gains of chicks fed a standard broiler starter diet at 7, 14, and 21 d of age were not significantly affected by previous dietary treatments for the dam. It was found that rooster semen volume and sperm concentration, viability, and motility were not affected by the feeding of contaminated diets. There was no effect of diet on the relative weights of liver, spleen, kidney, and testes. The feeding of contaminated grains decreased antibody titers against infectious bronchitis virus at the end of wk 12, and this was prevented by dietary supplementation with GMA. There was no effect of the diet on serum antibody titers against Newcastle disease virus. It was concluded that the feeding of blends of grains contaminated with Fusarium mycotoxins could affect

  2. Enzyme Supplementation of Broiler Feeds with Reduced Mineral and Energy Levels

    Directory of Open Access Journals (Sweden)

    JO Nunes

    2015-12-01

    Full Text Available ABSTRACT An experiment was conducted with the purpose of evaluating enzyme blends on the performance, carcass traits, and bone mineralization of broilers. In total, 928 one-day-old Cobb 500 male chicks of were used. A completely randomized design with four treatments with eight replicates of 29 birds each was adopted. The evaluated treatments were: 1- Positive Control (PC, feed containing the nutritional recommendations of the genetic company's manual; 2- Negative Control (NC, feed with reductions of 75 kcal/kg AME and 0.10 and 0.12 percent points of phosphorus and calcium, respectively; 3 - NC + enzyme blend (amylase + b-glucanase, xylanase, and phytase; 250 g/t of feed and 4 - NC + enzyme complex (phytase, amylase, xylanase, glucanase, pectinase, cellulase, and protease; 200 g/t of feed. Birds fed the diet with reduced nutrient levels (NC presented the worst performance (p0.05 carcass or parts yields. The broilers fed the reduced-nutrient and energy diet presented lower (p<0.05 tibial ash, calcium, and phosphorus contents that the other treatments. The use of enzyme combinations improved the performance of broilers fed diets with reduced nutrient and energy levels.

  3. Effects of turmeric (Curcuma longa) and vitamin E on histopathological lesions induced in bursa of Fabricius of broiler chicks by salinomycin.

    Science.gov (United States)

    Sayrafi, Reza; Mirzakhani, Navideh; Mobaseri, Reza

    2017-01-01

    The aim of this study was to evaluate the protective effects of the turmeric in comparison to vitamin E on bursal damages induced by salinomycin in broiler chickens. In this study, forty one day-old broiler chicks were randomly divided into four treatment groups: 1- basal diet as control, 2- basal diet plus salinomycin, 3- basal diet plus salinomycin (SLM) and vitamin E (Vit. E) and 4- basal diet plus salinomycin and turmeric powder. The chicks were treated for two weeks. At the end of the experiment, the bursal tissues were removed and fixed in 10% formalin solution. Tissue sections were stained with hematoxylin and eosin stain for histopathological studies. Light microscopic observations showed that, SLM diminished cortex thickness of bursal tissue, enhanced its medulla zone and caused severe lymphocytic necrosis. In addition, SLM led to fibrosis of interstitium along with sever edema of medulla zone in the bursal tissue of the chicken. Administration of Vit. E and TP significantly inhibited the SLM-induced derangements and comparing the Vit. E and TP showed no significant differences. The results of this study indicated that the turmeric may protect bursa of Fabricius against toxicity induced by salinomycin in chicks.

  4. Effects of feeding bentonite clay upon ochratoxin A-induced immunosuppression in broiler chicks.

    Science.gov (United States)

    Khatoon, Aisha; Khan, Muhammad Zargham; Abidin, Zain Ul; Bhatti, Sheraz Ahmed

    2018-03-01

    A presence of mycotoxins in feed is one of the most alarming issues in the poultry feed industry. Ochratoxins, produced by several Aspergillus and Penicillium species, are important mycotoxin regarding the health status of poultry birds. Ochratoxins are further classified into to several subtypes (A, B, C, etc) depending on their chemical structures, but ochratoxin A (OTA) is considered the most important and toxic. Bentonite clay, belonging to phyllosilicates and formed from weathering of volcanic ashes, has adsorbent ability for several mycotoxins. The present study was designed to study the effects of bentonite clay upon OTA-induced immunosuppression in broiler chicks. For this, 480 day-old broiler chicks were procured from a local hatchery and then different combinations of OTA (0.15, 0.3, or 1.0 mg/kg) and bentonite clay (5, 10, and 20 g/kg) were incorporated into their feed. At 13, 30, and 42 days of age, parameters such as antibody responses to sheep red blood cells, in situ lymphoproliferative responses to mitogen (PHA-P), and in situ phagocytic activity (i.e., via carbon clearance) were determined respectively. The results indicated there was a significant reduction of total antibody and immunoglobulin titres, lymphoproliferative responses, and phagocytic potential in OTA-treated birds, suggesting clear immunosuppression by OTA in birds in a dose-dependent manner. These results were also significantly lower in all combination groups (OTA with bentonite clay), suggesting few to no effects of feeding bentonite clay upon OTA- induced alterations in different immune parameters.

  5. Oxidative Stability and Physico-chemical Properties of Meat from Broilers Fed with Dietary Neem Leaf Powder, Spirulina and their Combination

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    Ravi KILLI

    2015-12-01

    Full Text Available This 6 week study was conducted to evaluate the antioxidant potential, maintaining quality and sensory properties of broiler meat from birds fed on dietary neem leaf powder (NLP. A total of 90 Vencobb broiler chicks were randomly allotted to 6 groups of 15 birds in each. Dietary treatments consisted of normal diet (control Group I, feed containing terramycin-200 (TM-200* at the concentration of 0.05% (Group II, feed containing NLP of 0.2% (Group III, feed containing NLP of 0.2% and spirulina of 1% (Group IV, feed containing TM-200 at 0.05% and spirulina of 1% (Group V and feed containing spirulina of 1% (Group VI. At the end of the experiment liver, kidney and muscle samples were collected to evaluate the tissue peroxidation (TBARS and protein carbonyls and antioxidant markers (SOD. Physico-chemical quality determinants of both fresh and preserved meat viz. extract release volume (ERV, water holding capacity (WHC and pH were also studied. TBARS protein carbonyls indicated a significant (P < 0.05 decrease in all the treated groups when compared to control. Superoxide dismutase levels were found to be significantly increased in all the treated groups, in all the tissues collected. Compared to control group, favorable physico-chemical quality determinants were recorded in all the treated groups. The sensory attributes did not show significant (P < 0.05 differences for color, flavor, juiciness, tenderness and overall acceptability. This study indicates enhanced stress tolerance levels, improved meat quality with unaffected consumer acceptance levels of the meat observed in the study, from broilers fed with neem and spirulina either alone or in combinationsș this points out that neem at 0.2% level can be used in poultry diets instead of antibiotic growth promoters (AGP.

  6. Evaluation of a new form of vitamin D (Hy. D) in improving growth performance and mineral utilization in broiler Chickens

    International Nuclear Information System (INIS)

    Ibrahim, S. B.; Blair, R.

    2007-01-01

    Two experiments were conducted to examine the effects of feeding diets containing 69μg/ kg of vitamin D 3 or 25-hydroxycholecalciferol (Hy.D), 0.45% (P100) or 0.35% (P50) National Research Council's available phosphorus (P) requirement with or without natuphos (phytase) on broiler chickens performance. The results of the first experiment (balance study) indicated no significant effects (P>0.05) in body weight, feed consumption and feed conversion ratio of broilers at 21 days of age. Tibia bone ash percentage was not effected by the dietary treatments. Chicks fed the P100 diet had a significantly (P<0.05) higher apparent P retention than those fed the P50 diet. The pH of different segments of intestine was not affected by dietary treatments. The phytase activities in the duodenal contents and tissues were significantly (P<0.05) higher in the chicks receiving diets with Hy.D in the absence of phytase. There were no differences in the growth performance of broilers under commercial conditions due to sources of vitamin D in the diets at 39 days of age.(Author)

  7. Effect of β-hydroxy-β-methylbutyrate calcium on growth, blood parameters, and carcass qualities of broiler chickens.

    Science.gov (United States)

    Qiao, X; Zhang, H J; Wu, S G; Yue, H Y; Zuo, J J; Feng, D Y; Qi, G H

    2013-03-01

    Beta-hydroxy-β-methylbutyrate (HMB), the metabolite of leucine, plays an important role in muscle protein metabolism. To investigate the effect of dietary HMB calcium (HMB-Ca) on growth performance, breast muscle development, and serum parameters in broiler chickens, a total of two hundred seventy 1-d-old Arbor Acres male broiler chicks were randomly allotted into 3 dietary treatments supplemented with 0, 0.05%, or 0.1% HMB-Ca during the starter (1 to 21 d) and grower (22 to 42 d) period. The results showed that broilers fed 0.1% HMB-Ca diet had higher ADG during the starter or the whole period, and gain 148 g more BW than the chicks fed the control diet at 42 d of age (P dressing percentage than birds fed the control or 0.05% HMB-Ca diet (P < 0.05). At 42 d of age, 0.1% HMB-Ca increased breast muscle yield than the control and decreased abdominal fat compared with the control or 0.05% HMB-Ca group (P < 0.05). In comparison with the control, feeding 0.1% HMB-Ca increased the triiodothyronine, thyroxine, triiodothyronine/thyroxine ratio and decreased the serum uric acid level at d 21 (P < 0.05). At 42 d of age, serum thyroxine level was elevated in the 0.05% HMB-Ca treatment, and the uric acid concentration was significantly decreased by the 0.1% HMB-Ca-supplemented diet (P < 0.05). Dietary HMB-Ca did not affect the growth hormone or insulin content. This study suggested that dietary supplementation of HMB-Ca improved growth performance, stimulated the breast muscle development, and decreased the abdominal fat deposition in broiler chickens, and the favorable effects were more pronounced in the starter phase. The growth promotion effect of HMB-Ca may be partly related to the increased serum thyroid hormones in broiler chickens.

  8. New possibilities for the utilization of slaughter-house by-products as protein sources in the nutrition of broiler chicks. [In Serbo-Crotian

    Energy Technology Data Exchange (ETDEWEB)

    Cuperlovic, M.; Jovanovic, M.; Bezbradica, L.

    1977-01-01

    Blood, rumen contents, and intestinal offal were dried after mixing with an organic carrier (bran or maize meal). Wheat bran was a good carrier for blood, simplifying its drying, and the resulting material had a high concentration of protein with good amino acid composition. The adsorptive qualities of maize meal were not as good as those of bran, but due to its higher level of energy, and lower crude fiber it was more convenient as a carrier for some by-products (i.e. rumen contents). The produced premixtures containing blood (dried with maize or bran), rumen content, and intestinal offal (combined with maize), were included in the diets for broilers, replacing fish meal and part of the soybean meal from the standard control diets. Diets containing 30% of blood-bran combination (supplying approx. 33% of total dietary protein) supported good final body weights of chicks (1.62 kg at 8 weeks of age), providing the diet contained sufficient protein and energy. The blood-maize premixtures, at 50% of the diet and supplemented with soybean meal and sunflower meal, also gave good results (1.68 kg finabody weight). Feeding diets containing 30 to 60% maize-rumen content premixtures (7 to 16% dry rumen content) gave lower final body weights of broiler chicks ( approx. 1.5 kg). Thus slaughter house by-products when dried as proposed could be successfully utilized in the nutrition of broiler chicks.

  9. Performance of broilers fed on diets containing different amounts of chaya (Cnidoscolus aconitifolius) leaf meal.

    Science.gov (United States)

    Sarmiento-Franco, L; McNab, J M; Pearson, R A; Belmar-Casso, R

    2002-05-01

    The performance and gut measurements of broilers fed on diets containing different amounts of chaya (Cnidoscolus aconitifolius) leaf meal (CLM) were examined in two experiments. In the first experiment, 60 Hubbard chickens (30 males and 30 females; 2 weeks old) were fed on five maize diets; these were formulated using 0, 150 (CLM150), 250 (CLM250) or 350 (CLM350) g CLM/kg, and the fifth diet contained soyabean. In the second experiment, 148 Ross male chicks, 1 day old, were fed on three isonitrogenous and isoenergetic maize-soyabean-based diets, which included 0 (control), 150 (C150) or 250 (C250) g CLM/kg. The diets were offered ad libitum for 2 or 3 weeks in the first and second experiments, respectively. Food intake, weight gain and the food:weight gain ratio were recorded. The weight of the gizzard and intestine and the weight and length of the caeca were also determined in the second experiment. In experiment 1, the birds fed on the maize-soyabean diet had a higher (p < 0.05) weight gain and final weight than birds fed on maize only or on the CLM150 diets. There were no differences for any of the variables studied between the birds fed on the maize-soyabean diet and those fed on the CLM250, nor between males and females. In the second experiment, weight gain, food intake and the food:weight gain ratio for birds fed on C250 were lower (p < 0.05) than those in birds fed on either the control or C150 diets. The weights of the gizzard and intestine were the lowest and the highest, respectively, in birds fed on C250 (p < 0.05). The length and weight of the caecum from birds fed on the control diet were lower (p < 0.05) than those of birds fed on either the C150 or C250 diets. The results from this study suggest that CLM may be included up to 150 g/kg in commercial diets without having an adverse effect on poultry performance, and may also be mixed with maize up to 250 g/kg to improve the performance of chickens fed on low-protein diets.

  10. Arsenic Induced Toxicity in Broiler Chicks and Its Amelioration with Ascorbic Acid: Clinical, Hematological and Pathological Study

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    Rabia Sharaf, Ahrar Khan*, Muhammad Zargham Khan, Iftikhar Hussain, Rao Zahid Abbas, S. T. Gul, Fazal Mahmood and Muhammad Kashif Saleemi

    2013-07-01

    Full Text Available This study was conducted to observe the arsenic (As toxicity lesions in birds and to know either Vit C ameliorates these toxic effects or not. One-day-old broilers chicks (n=72 procured from a local hatchery were randomly divided into four equal groups. First group was kept as control and second group was given As (50 mg/kg BW via crop tubing. Third group received in addition to As, Vit C (250 mg/kg BW whereas fourth group received only Vit C. Killing by neck dislocation of randomly selected six birds from each group was carried out on experimental days 0, 16 and 32 for collection of blood and tissues specimens. Arsenic treated birds showed clinical signs of toxicity throughout the experiment than all other groups. These clinical signs included decreased body weight and feed intake, dullness, open mouth breathing, increased thirst, ruffled feathers, pale comb, skin irritation and watery diarrhea which were not significant in any other group. As treated group showed a significant (P<0.05 decrease in hematological parameters. Severe gross and histopathological changes were observed in intestines, spleen and lungs of birds fed with As than all other groups. Decreased height of villi of middle portion of small intestines was also observed in As treated birds. Villi height in Vit C treated group increased as compared to control group. It was concluded that As induces severe toxic effects in broiler birds; however, these toxic effects can be partially ameliorated by Vit C.

  11. Evaluation of the effect of different wheats and xylanase supplementation on performance, nutrient and energy utilisation in broiler chicks

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    Gemma González-Ortiz

    2016-09-01

    Full Text Available The aim of this study was to evaluate the performance, nutrient utilisation and energy metabolism of broiler chicks fed 8 different wheat samples, supplemented or not with xylanase. Seven-hundred sixty eight male broilers (1-day-old were distributed to 16 experimental treatments (6 replicates per treatment. The treatments were in a factorial arrangement with 8 different wheats and 2 levels of xylanase (0 or 16,000 BXU/kg. The predicted apparent metabolisable energy (AME of the wheat samples ranged from 13.0 to 13.9 MJ/kg and all diets were formulated to contain the same amount of wheat. Body weight gain (BWG and feed intake (FI were measured at 21 d, as was jejunal digesta viscosity, and feed conversion ratio (FCR calculated. On day 24, one representative bird per pen was selected to calculate whole body energetics. At 21 d, 3 chicks per replicate were randomly allocated to metabolism cages for energy and nutrient utilisation determinations, and were continued on the experimental diets until 24-d-old. No interactions were observed for any performance response variables, ileal nutrient utilisation or digesta viscosity. Xylanase improved BWG and reduced FCR and digesta viscosity (P < 0.05. Wheat influenced dry matter (DM utilisation and xylanase increased ileal digestible energy (P = 0.04. Xylanase also improved (P < 0.05 DM and nitrogen retention. Apparent metabolisable energy and AME corrected for nitrogen (AMEn were subject to an interaction whereby wheats 2 and 6, which returned the lowest AME and AMEn values, responded to xylanase supplementation and the remainder did not. Net energy for production and the efficiency of energy use for production were not influenced by xylanase, but were affected by wheat (P < 0.05. Despite the significant differences between wheats with regards to their nutrient utilisation and energy metabolism in birds, xylanase removed this variance and resulted in more homogeneous performance.

  12. The Degree of Fat Unsaturation in the Tissue and Potential Immune Response of Broiler Fed Chlorella sp.

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    Sugiharto

    2010-05-01

    Full Text Available This study was carried out to investigate the effect of Chlorella sp. administered in the diet of broilers on the degree of fat unsaturation, ratio of EPA to AA in the tissue and potential immune response of broiler. A total of 90 heads of one-day-old Ross chicks were assigned in completely randomized design by 3 dietary treatments with 6 repetitions and 5 chicks in each pen. The diets were T1: control (basal diet without enrichment with Chlorella sp.; T2: basal diet enriched with 5-g of Chlorella sp./kg feed; T3: basal diet enriched with 10-g of Chlorella sp./kg feed. Skinless breast meat was sampled for FA determination at d-36. Chickens were vaccinated at d-6 and d-17 with live vaccine against ND to activate antibodies production, and then 2 ml of blood was collected at d-24 for IgG and IgM quantification. Administration of Chlorella sp. in broiler’s diet had no significant effect on the degree of fat unsaturation, the ratio between EPA and AA contained in the breast muscle and the concentration of IgG and IgM of broiler. In conclusion administration of Chlorella sp. from tropical marine origin in the diet of broiler has no significant effect on the degree of fat unsaturation, ratio of EPA to AA in the tissue, and potential immune response of broiler. The culture temperature in which the Chlorella sp. was cultivated may affect the FA composition of Chlorella sp. (Animal Production 12(2: 96-99 (2010Abbreviations: AA: arachidonic acid, ALA: α-linolenic acid, EPA: eicosapentaenoic acid, FA: fatty acids, LA: linoleic acid, PGE2: Prostaglandin E2, PUFA: polyunsaturated fatty acids, SFA: saturated fatty acidKey Words: antibody, broiler, Chlorella sp., fatty acid, PUFA

  13. A study of nutrient digestibility and growth performance of broiler chicks fed hairy and hairless canary seed (Phalaris canariensis L.) products.

    Science.gov (United States)

    Newkirk, R W; Ram, J I; Hucl, P; Patterson, C A; Classen, H L

    2011-12-01

    A nutrient retention study and a growth study were conducted with broiler chickens to evaluate the nutritive value and potential toxicity of 2 hairless canary seed products-hulled seed and groats (cultivar CDC Maria), and one hairy hulled canary seed (cultivar Keet). Each treatment was replicated 6 times (6 groups of 4 birds each). The hairless canary seed groat, hairless hulled canary seed, and the hairy hulled canary seed contained 24.5, 21.8, and 16.3% CP; 7.1, 5.8, and 6.6% ether extract; 1.5, 14.2, and 12.3% acid detergent fiber, and 3,867, 3,205 and 3,292 kcal/kg of AME(n), on a DM basis, respectively. The hairless canary seed groat, hairless hulled canary seed, and the hairy hulled canary seed protein comprised, respectively, 0.49, 0.33, and 0.33% lysine (DM basis), which was 79, 78, and 67% digestible (apparent ileal); 0.65, 0.53, and 0.60% cysteine (DM basis), which was 86, 87, and 85% apparent ileal digestible; and 0.40, 0.30, and 0.25% methionine (DM basis), which was 89, 90, and 86% apparent ileal digestible. In the second study, a 35-d feeding study with male broiler chickens was conducted. The canary seed products were compared with a Canadian Western Red Spring wheat control. Each treatment was replicated 6 times (6 groups of 4 birds each). The test ingredients comprised 50% of the corn/soybean diets. The birds fed the hulled canary seed (hairy or hairless) had similar weight gain, feed intake, and G:F to those fed wheat. There were no statistically significant (P = 0.05) differences in the weights of the bursa, heart, kidneys, liver, spleen, or the pancreas, nor was there any effect on serum lactate dehydrogenase or creatine kinase. The data indicated that feeding hulled canary seed increased the number of gizzard ulcers (P < 0.01). It was concluded that canary seed does not contain anti-nutritional components that negatively affect broiler performance or bird health. However, the canary seed hulls may damage the gizzard lining.

  14. Broiler incubation. 1. Effect of elevated temperature during late incubation on body weight and organs of chicks.

    Science.gov (United States)

    Leksrisompong, N; Romero-Sanchez, H; Plumstead, P W; Brannan, K E; Brake, J

    2007-12-01

    Three experiments were conducted to investigate the effect of increased egg temperature during the final third of incubation on BW, yolk sac, heart, and digestive organs of broiler chicks at hatching. Egg temperatures were found to be approximately 1.0 to 1.5 degrees C higher than incubator air temperature. Elevated egg temperature (39.5 degrees C) after embryonic day 14 generally accelerated hatching time but decreased the relative weight of the heart in all 3 experiments, whereas BW and relative weights of the gizzard, proventriculus, and small intestines were significantly smaller in 2 of 3 experiments as compared with the control (approximately 38.2 degrees C). Relative weights of the yolk sac or liver were significantly larger due to elevated egg temperature in single experiments only. A striking feature of the chicks that developed at an elevated egg temperature was their white color as compared with the yellow color of chicks from eggs incubated at more normal temperatures.

  15. The effects of direct-fed microbial supplementation, as alternative to antibiotics, on growth performance, intestinal immune status and epithelial barrier protein expression in broiler chickens

    Science.gov (United States)

    This study was conducted to investigate the effects of Bacillus subtilis supplementation in broiler chicken diets on growth performance, feed efficiency, intestinal cytokine and tight junction (TJ) protein mRNA expression. Day-old broiler chicks (n = 140) were assigned five dietary treatments: basal...

  16. The influence of different phosphorus supply on phosphorus turnover in growing broiler chicks by means of 32P isotope

    International Nuclear Information System (INIS)

    AL-Masri, M.R.; Guenther, K.D.

    1988-01-01

    In two experiments four diets were fed to male broiler chicks from day 10 to 29 of age ad libitum. The basal diet contained 0,32% P from inorganic origin (group IV). All other diets were supplemented up to 0,65% P with Monocalciumphosphate (MCP, group I), MCP + Na-phytate (group II) and Na-phytate (group III). By addition of CaCO 3 an equal Ca/P ratio of 1,6:1 was adjusted in the diets. At 14 days of age the chicks were injected with 32 P-NaPO 4 in the right leg (1,480 KBq). P retention was calculated following the comparative slaughter technique. Isotope dilution technique was used to determine endogenous fecal and renal P excretion. From retention and endogenous excretion P turnover within the animal was derived. The influence of feed P source on endogenous P excretion is higher than the influence of total P in the diet. Elevated inorganic P content of the diet effects increased P turnover and endogenous excretion. A comparison of relative P excretion and availability of feed P (group I = 100) in the four treatments resulted in: 100, 67, 35, 15 or 100, 72, 36, 59, respectively. 29%, 34%, 35% or 64% of available P were retained, respectively. The ratio of relative retention to relative endogenous excretion of absorbed P was 0,41, 0,52, 0,54, 1,78 in the four treatments. 20 refs., 3 figs., 8 tabs. (author)

  17. IMMUNO-MODULATORY EFFECT OF INACTIVATED EIMERIA TENELLA VACCINE AND LIVE IMPPORTED COCCIDIAL VACCINE ON NEWCASTLE DISEASE VIRUS VACCINA TED BROILER CHICKS

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    Muhammad Akram Muneer, Haji Ahmad Hashmi, Masood Rabbani, Zahid Munir Chaudhry and Ali M. Bahrami

    2001-01-01

    Full Text Available A total of 160 one-day-old broiler chicks were used to evaluate the immunomodulatory effects of an inactivated Eimeria tenella vaccine and a live polyvalent imported antiococcidial vaccine (Coccivac. This study indicated that both of these vaccines did not adversely affect the development of serum antibody against Newcastle disease virus (NDV and the chicks vaccinated with either of the anticoccidial vaccines resisted the virulent NDV challenge. A study of the lymphoid organs such as bursa of fabricuis: thymus and spleen from the experimental chicks indicated that those organs were comparable with those from the chicks not vaccinated with these coccidial vaccines. The overall findings of this study indicate that anticoccidial vaccines do not have any effects on the immune functions of the vaccinates. In fact these vaccines prevented the occurrence of clinical coccidiosis in the vaccinates.

  18. Effect of High Phytase Inclusion Rates on Performance of Broilers Fed Diets Not Severely Limited in Available Phosphorus

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    T. T. dos Santos

    2013-02-01

    Full Text Available Phytate is not only an unavailable source of phosphorus (P for broilers but it also acts as an anti-nutrient, reducing protein and mineral absorption, increasing endogenous losses and reducing broiler performance. The objective of this study was to investigate the anti-nutritional effects of phytate by including high levels of phytase in diets not severely limited in available P. A total of 768 male Arbor Acres broilers were distributed in six treatments of eight replicate pens of 16 birds each consisting of a positive control diet (PC, positive control with 500 FTU/kg phytase, negative control (NC diet with lower available P and calcium (Ca levels and the same NC diet with 500, 1,000 or 1,500 FTU/kg phytase. Body weight gain (BWG, feed intake (FI, feed conversion ratio (FCR and mortality were determined at 21 and 35 d of age while foot ash was determined in four birds per pen at 21 d of age. FI, FCR and foot ash where not affected by the lower mineral diets at 21 d of age nor by the enzyme inclusion but broilers fed lower Ca and available P diets had lower BWG. At 35 d of age no difference was observed between broilers fed the positive or NC diets but broilers fed 500, 1,000 and 1,500 FTU/kg on top of the NC diet had better FCR than broilers fed the positive control diet. When compared to birds fed a diet adequate in P, birds fed the same diet included with 500, 1,000 and 1,500 FTU/kg of phytase in marginally deficient available P and Ca diets had an improvement of performance. These results support the concept that hydrolysing phytate and reducing the anti-nutritional effects of phytate improves bird performance on marginally deficient diets that were not covering the P requirement of birds.

  19. Litter characteristics and pododermatitis incidence in broilers fed a sorghum-based diet

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    CMC Carvalho

    2014-09-01

    Full Text Available The aim of this study was to evaluate the incidence of footpad dermatitis and quality of broilers litter fed with sorghum grain and diets based in corn. It was used 544 male and female chicks, distributed in a completely randomized design with two treatments and eight replications. The chicken feed was formulated and produced from corn, soybean meal and sorghum grain. In the formulation were kept constant levels of energy and protein in accordance with the following treatments: A. Control (diet based on corn and soybean meal; B. Grain sorghum (Whole Sorghum + soybean meal. At 35 and 42 days were evaluated mineral matter (A %, calcium (Ca%, phosphorus (P%, pH and dry matter (DM % of the poultry litter. To evaluate the footpad dermatitis were evaluated eight feet per treatment by visual analysis. At 35 and 42 days of age DM % MM (%, Ca (% P (% and pH of poultry litter no difference (p>0.05 was found between the treatments and the type of ingredient in the birds' diet is not related (p>0.05 with the incidence of footpad dermatitis. The incidence of footpad dermatitis and the quality of the litter weren't influenced by the type of the ingredient used in diet.

  20. Experimental rickets in broilers: gross, microscopic, and radiographic lesions. III. Vitamin D deficiency

    International Nuclear Information System (INIS)

    Long, P.H.; Lee, S.R.; Rowland, G.N.; Britton, W.M.

    1984-01-01

    Day-old broiler chicks were fed a vitamin D-deficient diet for 30 days. Gross, microscopic, and radiographic examinations of the proximal tibiotarsus were done at 2, 3, and 4 weeks of age. In 2-week-old chicks, there was variable lengthening and disorganization of the proliferating-prehypertrophied zone (P-PHZ), resorption of cartilage spicules in the degenerating hypertrophied zone and primary spongiosa, and lengthening of bone spicules in the secondary spongiosa; many bone surfaces were lined with increased osteoid. In 3-week-old chicks, there was a tendency for the P-PHZ to decrease in relative length and for cartilage spicules to increase in length. In 4-week-old chicks, however, there was marked lengthening of the P-PHZ, resorption of cartilage spicules, and replacement of the metaphysis with irregularly oriented islands of woven bone, osteoid, and loose fibrous connective tissue

  1. Reduced Nutrient Excretion and Environmental Microbial Load with the Addition of a Combination of Enzymes and Direct-Fed Microbials to the Diet of Broiler Chickens

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    MFFM Praes

    2016-03-01

    Full Text Available Abstract This study evaluated the effects of the dietary inclusion of an enzyme blend and a direct-fed microbials in broiler diets on litter production and quality. In total, 900 Cobb 500(r broiler chicks were distributed according to a completely randomized design into 4 treatments and 9 replicates of 25 birds each. Broilers were reared from 1 to 42 days of age. The treatments consisted of the following diets: NC: negative control; DFM: NC + 500 ppm of direct-fed microbials product (DFM, containing Bacillus subtilis and Bacillus licheniformis; ENZ: diet formulated with an enzyme blend (20 ppm phytase, 200 ppm protease and 200 ppm of xylanase; DFM+E: ENZ + DFM. Birds and litter were weighed at the start and end of the rearing period, for litter production and waste ratio (Rw determination. Litter samples were analyzed for dry matter (DM content, total and thermotolerant coliform counts, nutrient composition (nitrogen (N, phosphorous (P and potassium (K, and fiber fraction (neutral detergent fiber (NDF, acid detergent fiber (ADF and lignin. The dietary inclusion of the evaluated additivesdid not influence litter production or Rw; however, ADF (%, NDF (kg and kg/kg DM litter, and total and thermotolerant coliform counts were reduced, and N content increased in the litter. The diets containing enzymes (ENZ and DFM+E reduced litter P content. The addition of exogenous enzymes and their combination with a DFM based on Bacillus spp .Did not affect waste production, and reduced litter microbial load, and the contents of P and insoluble fiber in the litter.

  2. The increase of Lactobacillus species in the gut flora of newborn broiler chicks and ducks is associated with weight gain.

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    Emmanouil Angelakis

    Full Text Available BACKGROUND: A bacterial role in the obesity pandemic has been suspected based on the ingestion of probiotics that can modify the gut flora. The objective of our study was to determine if increased Lactobacillus sp. in the gut flora of newborn broiler chicks and ducks could result in weight gain increase. METHODOLOGY: Female broiler chicks (Gallus gallus domesticus and ducks (Anas platyrhynchos domestica were separated into one control and two experimental groups, and inoculated once or twice with 4x10(10Lactobacillus spp. per animal in PBS, or with PBS alone. Fecal samples were collected before and at 24 hours, 2, 4, 8, 16 and 30 days after the inoculation. DNA was extracted from the stools, and qPCR assays were performed on a MX3000 system for the detection and quantification of Lactobacillus sp., Bacteroidetes and Firmicutes, using a quantification plasmid. Animals were measured and sacrificed 60 days after the beginning of the experiment, and livers were collected and measured. PRINCIPAL FINDINGS: Chicks inoculated once and twice with Lactobacillus weighed 10.2% (p = 0.0162 and 13.5% (p = 0.0064 more than the control group animals, respectively. Similarly, ducks inoculated once and twice weighed 7.7% (p = 0.05 and 14% (p = 0.035 more than those in the control group, respectively. Liver mass was also significantly higher in inoculated animals compared to the control group. Inoculation with Lactobacillus sp. increased the DNA copies of Lactobacillus spp. and Firmicutes in the stools. Bacteroidetes remained stable, and only the second Lactobacillus sp. inoculation significantly decreased its population in chicks. The ratio of DNA copies of Firmicutes to those of Bacteroidetes increased to as much as 6,4 in chicks and 8,3 in ducks. CONCLUSIONS: Differences in the intestinal microbiota may precede weight increase, as we found that an increase of Lactobacillus sp. in newborn ducks and chicks preceded the development of weight gain.

  3. The influence of increasing dietary fat or protein on the efficiency of broiler chickens exposed to low levels of gamma-rays

    International Nuclear Information System (INIS)

    Ezzat, I.E.; Ghazalah, A.A.

    2002-01-01

    A total of 400 one-day-old chicks, from hubbard line of meat production type, were used in this study. The chicks were divided into three nutritional groups. The second group was fed on a high protein feed containing 3% protein higher than that of the normal diet (group one). The fat level was increased by 4% in the feed of the third group over the value of the normal diet. Each group was divided again into four subgroup treatments (To, T1, T2, and T3) according to the exposure to gamma rays in a single or multi doses. The aim of this study was to compare between the nutritional types and its combinations with the applied doses of gamma rays on broiler performance. The results can be summarized as follows: The broiler chicks fed on the high fat ration and exposed to 0.25 Gy of gamma-rays four times (group 111, T3) recorded the highest body weight gain along the duration of the experiment. in general, the stimulation effect of the gamma-ray doses applied herein was obvious during the period of rapid growth (0-4 weeks of age) then it was followed by retardation in growth rate. The group of chicks fed on a diet containing high fat (group 111), achieved the crude fiber and nitrogen extract digestibility's. Increase of dietary protein was accompanied by a significant increase in carcass protein, water and ash content. Carcass fat was increased while carcass water was decreased with low level of gamma rays

  4. Effect of dietary supplementation with onion (Allium cepa L. on performance, carcass traits and intestinal microflora composition in broiler chickens

    Directory of Open Access Journals (Sweden)

    Majid Goodarzi

    2014-02-01

    Full Text Available Objective: To examine the effect of onion (Allium cepa L. as an antibiotic growth promoter substitute on growth performance, carcass traits, and microflora composition in broiler chickens. Methods: A total of 192 one-day old mixed sex broiler chicks (Ross 308 were weighed and randomly allocated to four treatment groups, each with 4 replicate pens of 12 chicks. The dietary treatments consisted of the basal diet (control, antibiotic (15 mg virginiamycin/kg, and control+10 or 30 g fresh onions bulb/kg diet. Body weights of broilers were determined at Day 1, 21, and 42, feed intake was determined at the same periods, and feed conversion ratio was calculated accordingly. At Day 42, two birds per replicate were slaughtered for determination of carcass and organ weights. The populations of Lactobacilli spp. and Escherichia coli were enumerated in ileum by conventional microbiological techniques using selective agar media. Results: Dietary supplementation of 30 g/kg onion increased final body weight of broilers at 42 d of age compared to that of the other treatments (P<0.05. Birds fed 30 g onion/kg in the diet had the highest feed intake than other treatments at different growth periods (P<0.05. Feed conversion ratio, carcass yield and internal organ weights were not affected by the dietary treatments at Day 42. The Lactobacilli spp. population in birds supplemented with onion at the level of 30 g/ kg significantly was higher than other groups at 42 d of age (P<0.05. The lowest Escherichia coli loads were detected in broilers fed diets containing 15 mg virginiamycin/kg. The Escherichia coli loads significantly decreased in broilers fed diets containing 10 or 30 g onion/kg (P<0.05. Conclusions: In conclusion, the results of the current study indicated that supplementing broiler diet with 30 g onion/kg could induce favorable influences on performance and ileum microflora composition.

  5. Growth performance and carcass and meat quality of broiler chickens fed diets containing micronized-dehulled peas (Pisum sativum cv. Spirale) as a substitute of soybean meal.

    Science.gov (United States)

    Laudadio, V; Tufarelli, V

    2010-07-01

    An experiment was carried out to evaluate the effects of diets containing peas on productive traits, carcass yields, and fatty acid profiles (breast and drumstick meat) of broiler chickens. Hubbard strain broiler chicks, divided into 2 groups, received from 14 d to slaughtering age (49 d) a wheat middlings-based diet containing soybean (190 g/kg) or micronized-dehulled peas (400 g/kg) as the main protein source. The inclusion of peas did not significantly change the growth performance of birds. The pea level had no effect on the dressing percentage, the percentage of breast or drumstick muscles, and abdominal fat. The muscles of birds fed the pea diet had significant (P < 0.05) lower L* (lightness) and b* (yellowness, drumstick muscle) values and fat content. Instead, total collagen and water-holding capacity values were higher in the pea treatment. The polyunsaturated fatty acid concentration in breast and drumstick muscles was significantly increased with the alternative protein source inclusion, whereas the saturated fatty acid was similar among treatments. The n-6/n-3 polyunsaturated fatty acid ratio of the broiler drumstick meat decreased significantly in the pea group. Dietary pea inclusion improved the saturation index of meat without altering atherogenic and thrombogenic indexes. It can be concluded that the pea treatment tested had a positive effect on the performance and meat quality of broiler chickens.

  6. Bacillus Coagulans Enhance the Immune Function of the Intestinal Mucosa of Yellow Broilers

    Directory of Open Access Journals (Sweden)

    L Xu

    Full Text Available ABSTRACT This experiment was conducted to investigate the effects of Bacillus coagulans on the growth performance and immune functions of the intestinal mucosa of yellow broilers. Three hundred and sixty one-day-old yellow chicks were randomly allocated to four treatments groups with six replicates of 15 chicks each. The broilers were randomly subjected to one of the following treatments for 28 days: control group (group1, fed a basal diet and three treatments (group 2, 3, 4 fed the basal diet supplemented with 100, 200, or 300 mg/kg Bacillus coagulans , respectively. The results showed that for 28 days, compared with the control diet, the dietary addition of 200 mg/kg Bacillus coagulans significantly decreased the feed/gain ratio (F/G (p<0.05, improved the thymus index, spleen index and bursa index (p<0.05, increased the villus height to crypt depth ratio (V/C in the duodenum (p<0.05, increased the number of secretory immunoglobulin (sIgA positive cells ( p<0.05. The dietary addition of 200 mg/kg Bacillus coagulans promoted a significant increase in Lactobacillus spp. populations and suppressed Escherichia coli replication in cecum, compared with the control (p<0.05. Moreover, the dietary addition of 200 mg/kg Bacillus coagulans also significantly enhanced the levels of interferon alpha (IFNα, toll-like receptor (TLR3, and melanoma differentiation-associated protein 5(MDA5 in the duodenum (p<0.05. In conclusion, the dietary addition of Bacillus coagulans significantly improved broiler performance, and enhanced the intestinal mucosal barrier and immune function. The optimal dosage of Bacillus coagulans for yellow broilers was determined as 2×108 cfu/kg.

  7. Effect of feeding different levels of corn snack waste on broiler ...

    African Journals Online (AJOL)

    This study was conducted to investigate the effect of using corn snack waste on growth performance, carcass traits and economical efficiency of broiler chicks. Five hundred Ross 308 day-old broiler chicks were divided randomly into 20 pens. There were 25 chicks in each pen which consists of 4 treatments (4 levels of waste ...

  8. Responses of broiler chickens under hot humid tropical climate as ...

    African Journals Online (AJOL)

    NLP) on the growth, haematology and serum biochemistry parameters of broiler chicks. Two hundred and forty day-old broiler chicks were randomly assigned to four treatments which contained 0, 5, 10 and 15g/Kg diets in a Completely ...

  9. The effect of direct-fed microbial supplementation, as an alternative to antibiotics, on growth performance, intestinal immune status and epithelial barrier protein expression in broiler chickens

    Science.gov (United States)

    The objective of this study was to investigate the effects of Bacillus subtilis-based probiotic supplementation in broiler chicken diets on growth performance, feed efficiency, intestinal cytokine and tight junction (TJ) protein mRNA expression. Day-old broiler chicks (n = 140) were randomly assigne...

  10. Effects of Lipotropic Products on Productive Performance, Liver Lipid and Enzymes Activity in Broiler Chickens

    Directory of Open Access Journals (Sweden)

    Khosravinia H

    2015-12-01

    Full Text Available In a 42-d experiment, 576 one-day-old Vencobb 308 broiler chicks were used to investigate the effects of lecithin extract (0.5 g/kg, choline chloride 60% (1 g/kg and Bio choline (1 g/kg in diets of moderate and high energy in a 4 × 2 factorial arrangement on performance and certain physiological traits in broiler chickens. The inclusion of Bio choline and lecithin extract in the diet significantly increased average daily gain and improved feed conversion ratio  in overall (1 to 42 d period (P < 0.05. Performance efficiency index was improved in the birds fed with Bio choline compared to those fed control diet. Broilers fed diets containing Bio choline and lecithin extract had less abdominal fat percentage than those fed choline chloride or control diet. Regardless of dietary energy level, supplementation of diet with Bio choline, choline chloride and lecithin extract significantly decreased liver lipid concentration (P < 0.05. Aspartate aminotransferase activity increased in the serum of broilers fed high energy diets while it was decreased in the birds received diets containing choline chloride. Lipotropic compounds decreased serum aspartate aminotransferase activity in the birds fed on high energy diets. The addition of Bio choline and lecithin extract to diet significantly decreased serum γ–glutamyltransferase activity (P < 0.05. Results of the present study revealed that dietary supplementation of commercial lipotropic compounds could remove potential detrimental effects from high energy diets through reducing liver fat and maintaining liver health.

  11. Deleterious effects of magnesium intoxication upon the domestic broiler chick

    Energy Technology Data Exchange (ETDEWEB)

    Lee, S.R.

    1984-01-01

    Dietary levels of 0.6 to 0.8% magnesium in a corn-soy basal were rachitogenic. These rickets appeared most like phosphorus deficiency. Bone Ca/P ratios were numerically quite low implying a lack of transformation from amorphous calcium phosphate to hydroxyapatite. Bone alkaline phosphatase activity was elevated. Additional dietary phosphorus ameliorated, but could not overcome the rachitogenic effects of magnesium. Tibial dyschondroplasia (TD), induced by elevated dietary P, was decreased by high levels of dietary Mg, but with no decrease in plasma phosphorus. Anticoccidial ionophores fed in conjunction with a moderate dietary challenge of Mg (0.48%) produced no clear changes in plasma calcium or Mg, but did interact to reduce body weight. The diarrhea caused by magnesium intoxication is not due to hyperosmotic loads of Mg per se. Rather, Cl was observed to be the major ionic constituent of the gut osmotic load implying different gut ionic fluxes in control versus magnesium intoxicated chicks. These data imply that the cathartic action of Mg is due to hypersecretion of the gut. Effects mediated or modified by the CNS changed in magnesium intoxicated chicks. Such chicks appeared cold and stayed near the heat. When startled, they exhibited extreme avoidance behavior and seizures similar to epilepsy and/or Cl deficiency. Brain tissue Mg content did not concomitantly increase. Whether these effects are due to central signals is unclear.

  12. Water-soluble phosphorus in fresh broiler litter is dependent upon phosphorus concentration fed but not on fungal phytase supplementation.

    Science.gov (United States)

    Applegate, T J; Joern, B C; Nussbaum-Wagler, D L; Angel, R

    2003-06-01

    This experiment determined the effects of different phosphorus (P) feeding programs on total and water-soluble P excretion by broilers. Ross 308, male broilers were fed an industry (IND) diet (0.48, 0.35, 0.31, and 0.30% nonphytate P; NPP), an industry diet with reduced NPP and supplemental phytase [IND + PT; 600 phytase units (FTU)/kg; 0.36, 0.26, 0.20, and 0.19% NPP], a diet to more closely meet the birds' NPP requirements in which NPP was reduced further with supplemental phytase (REQ + P; 600 FTU/kg; 0.36, 0.26, 0.19, and 0.09% NPP), or a diet with low-phytate(lpa 1-1) corn with supplemental phytase (LPA + P; 600 FTU/kg; 0.37, 0.29, 0.19, 0.19% NPP). These diets were fed from hatch to 17 d, 17 to 31 d, 31 to 42 d, and 42 to 49 d of age, respectively. Fungal phytase was analyzed prior to diet formulation. Diets were fed to six replicate pens of 39 birds per pen. Litter samples were collected at 49 d of age and frozen prior to analyses. Diet did not significantly affect broiler performance (average BW at 49 d = 3.03 kg), tibia, or toe ash throughout the study (P > 0.05). Litter from broilers fed the IND diet was significantly higher (P litter from broilers fed IND + PT (0.84 and 0.14% of DM, respectively), REQ + P (0.78 and 0.11% of DM, respectively), or LPA + PT (0.64 and 0.12% of DM, respectively). Litter total and water-soluble P were not significantly different among broilers fed IND + PT, REQ + PT, or LPA + PT. In conclusion, phytase supplementation did not affect the solubility of P in the litter regardless of P feeding program.

  13. Prediction of ingredient quality and the effect of a combination of xylanase, amylase, protease and phytase in the diets of broiler chicks. 2. Energy and nutrient utilisation.

    Science.gov (United States)

    Cowieson, A J; Singh, D N; Adeola, O

    2006-08-01

    1. In order to investigate the effects of xylanase, amylase, protease and phytase in the diets of broiler chickens containing graded concentrations of metabolisable energy (ME), two 42-d experiments were conducted using a total of 2208 broiler chicks (8 treatments with 12 replicate pens in each experiment). 2. Four diets including one positive and three negative control diets were used. Three maize/soybean meal-based negative control (NC) diets were formulated to be identical in available phosphorus (P), calcium (Ca) and amino acids but NC1 contained approximately 0.17 MJ/kg less ME than NC2 and approximately 0.34 MJ/kg less ME than NC3. A positive control (PC) was fed for comparison and was formulated to be adequate in all nutrients, providing approximately 0.63 MJ/kg ME, 0.13% available P, 0.12% Ca and 1 to 2% amino acids more than NC1. 3. The reduction in nutrient density between NC1 and PC was determined using ingredient quality models Avichecktrade mark Corn and Phychecktrade mark that can predict the response to exogenous enzymes in maize/soybean meal-based broiler diets. Supplementation of each diet with or without a cocktail of xylanase, amylase, protease and phytase gave a total of 8 dietary treatments in a 4 x 2 factorial arrangement. The same treatments and diet designs were used in both experiments but conducted in different locations using different batches of maize, soybean meal and minor ingredients. 4. In both experiments, digestibility was improved by the addition of exogenous enzymes, particularly those for P, Ca and certain amino acids. In addition, the supplementation of the PC with enzymes elicited a positive response indicating that over-the-top addition of xylanase, amylase, protease and phytase may offer a nutritionally and economically viable alternative to feed cost reduction. 5. It can be concluded that the digestibility of nutrients by broilers fed on maize/soybean meal-based diets can be improved by the use of a combination of xylanase

  14. Influence of post hatch dietary supplementation of fat on performance, carcass cuts and biochemical profile in Ven Cobb broiler

    Directory of Open Access Journals (Sweden)

    Komal Prasad Rai

    2015-02-01

    Full Text Available Aim: The present experiment was conducted to study the effect of post hatch dietary fat supplementation on performance of broiler chicken. Materials and Methods: A total of 120 day-old Ven Cobb broiler chicks were randomly assigned to 4 treatment groups of 30 chicks in each (three replicates of 10 birds/treatment. The trial lasted for 35 days. The experimental design was a completely randomized design. Four types of diet were formulated for 1st week: T1, T2, T3 and T4 contained control diet with no added fat, 2.5, 5 and 7.5% fat, respectively. After 1st week post-hatch period chicks were fed ad libitum with the normal basal diet as per Bureau of Indian Standard recommendations till completion of the experiment (8-35 days. Results: Significantly higher (p<0.05 body weight and improved feed conversion ratio (FCR was recorded in birds fed 5% dietary fat at the end of the experiment whereas, feed intake was not significantly affected. Significantly (p<0.05 higher dressed weight was observed due to 5% fat supplementation than other groups whereas, it was not significant for other carcass cuts. No significant differences were observed in moisture, protein and lipid content of breast and thigh muscle of broiler due to supplemented fat whereas, 2.5% dietary fat significantly (p<0.05 increase the serum HI titer on day 28th. In biochemical profile, higher serum albumin (g/dl was recorded due to 5% fat supplementation whereas other biochemical components did not show any significance difference among treatments. Conclusion: It may be concluded that supplementation of fat in broilers diet improves the overall FCR, dressing percentage and gain more body weight.

  15. Effects of different limestone particle sizes in the diet of broiler breeders post molting on their performance, egg quality, incubation results, and pre-starter performance of their progeny.

    Science.gov (United States)

    Bueno, I J M; Surek, D; Rocha, C; Schramm, V G; Muramatsu, K; Dahlke, F; Maiorka, A

    2016-04-01

    An experiment was conducted to test the hypothesis that a coarse limestone diet improves productivity, reproductive performance and the calcium utilization of molted broiler breeders. In total, 640 broiler breeder females, 73-week-old and sixty-four 27-week-old cockerels, Cobb 500, were evaluated during 10 weeks, according to a randomized block design composed of 4 treatments with 8 replicates each. Treatments consisted of diets with the inclusion of 100% fine limestone-fine PS (0.2 mm GMD-geometric mean diameter); PS1: 30% fine limestone+70% limestone with 1.0 mm GMD; PS2: 30% fine limestone+70% limestone with 2.0 mm GMD; and PS3: 30% fine limestone+70% limestone with 3.0 mm GMD. Calcium retention in the gizzard of the breeders, bone characteristics, and breeder performance, egg characteristics, eggshell quality, incubation performance, chick quality and yield, chick pre-starter live performance, and chick bone characteristics were determined. There was no significant difference (P>0.05) in the rate of lay, percentage of non-settable eggs, egg weight, egg shape index, egg specific gravity, eggshell weight, thickness, and percentage hatchability and egg weight loss of broiler breeders fed with diets with different limestone particle sizes. The chick quality and yield, chick pre-starter live performance, and chick bone characteristics were not affected (P>0.05) by any of the limestone particle sizes. It was concluded that live and reproductive performance parameters of broiler breeders post molting is not affected by limestone particle size in the feed. © 2016 Poultry Science Association Inc.

  16. Effect of super dosing of phytase on growth performance, ileal digestibility and bone characteristics in broilers fed corn-soya-based diets.

    Science.gov (United States)

    Manobhavan, M; Elangovan, A V; Sridhar, M; Shet, D; Ajith, S; Pal, D T; Gowda, N K S

    2016-02-01

    A feeding trial was designed to assess the effect of super dosing of phytase in corn-soya-based diets of broiler chicken. One hundred and sixty-eight day-old broilers were selected and randomly allocated to four dietary treatment groups, with 6 replicates having 7 chicks per treatment group. Two-phased diets were used. The starter and finisher diet was fed from 0 to 3 weeks and 4 to 5 weeks of age respectively. The dietary treatments were consisted of normal phosphorus (NP) group without any phytase enzyme (4.5 g/kg available/non-phytin phosphorus (P) during starter and 4.0 g/kg during finisher phase), three low-phosphorus (LP) groups (3.2 g/kg available/non-phytin P during starter and 2.8 g/kg during finisher phase) supplemented with phytase at 500, 2500, 5000 FTU/kg diet, respectively, to full fill their phosphorus requirements. The results showed that super doses of phytase (at 2500 FTU and 5000 FTU/kg) on low-phosphorus diet improved feed intake, body weight gain, ileal digestibility (serine, aspartic acid, calcium, phosphorus), blood P levels and bone minerals such as calcium (Ca), P, magnesium (Mg) and zinc (Zn) content. It could be concluded that super doses of phytase in low-phosphorus diet were beneficial than the normal standard dose (at 500 FTU/kg) of phytase in diet of broiler chicken. Journal of Animal Physiology and Animal Nutrition © 2015 Blackwell Verlag GmbH.

  17. Ameliorative effect of mycofix plus 3.0 in reducing intensity of Eimeria tenella infection during aflatoxicosis in broiler chicks

    Directory of Open Access Journals (Sweden)

    A. M. Shareef

    2008-01-01

    Full Text Available One hundred and sixty male broiler chicks were fed at one day of age aflatoxin (AF at a rate of 3.5 mg/kg alone, or with groups injected with Eimeria tenella sporulated oocysts (40000 at 14 days of age. Adsorbent (Mycofix® plus 3.0 was incorporated at a rate of 0.25% in the above mentioned groups from one day of age till the end of the experiment. The study was conducted to reveal the effect of a aforementioned different diets and treatments on live body weight, feed consumption, feed conversion ratio, blood parameters (total red blood cells, hemoglobin, packed cell volume, biochemical profile of serum (alkaline phosphatase and β-carotin, liver weights, bursal and thymus indexes, caecal lesion scores and mortalities. The results indicated that AF was responsible for a significant (P<0.05 reduction. in body weigh gain (BWG, feed consumption, and an increase in feed conversion ratio. Afllatoxin was also responsible for reduction in blood parameters, β-Carotin, bursal and thymus indexes. While relative liver weight and alkaline phosphatase level were significantly (P<0.05 increased. Groups that fed AF at a rate of 3.5 mg/kg feed and exposed to sporulated oocysts of Eimeria tenella show a high significant (P<0.05 reduction in BWG, feed consumption and an increase in feed conversion ratio. Aflatoxin was also responsible for significant blood parameter, β-carotin, and also a significant (P<0.05 increase in caecal lesion scores, mortality, alkaline phosphatase level and relative liver weight, while they showed significant (P<0.05 decrease in bursal and thymus indexes in comparison with injected groups with E-tenella sporulated oocysts alone. The study approved that the groups maintained on mycofix plus 3.0 (0.25% and contaminated with aflatoxin 3.5 mg/kg, revealed a positive noticeable effects in amelioration on BWG, feed consumption and feed conversion, blood parameter, β-carotin, alkaline phosphatase level, relative liver weight, bursal and

  18. Changes in selected serum parameters of broiler chicken fed supplemental chromium.

    Science.gov (United States)

    Króliczewska, B; Zawadzki, W; Dobrzanski, Z; Kaczmarek-Oliwa, A

    2004-12-01

    The present study was conducted to evaluate the effect of chromium (Cr) from Cr yeast on the growth performance and total cholesterol, high-density lipoprotein (HDL) and low-density lipoprotein (LDL) cholesterol, triglycerides, glucose, total protein and Cr concentration in the serum of broiler chicken. The birds were fed a control diet or a control diet supplemented with Cr at a level of 300, 500 microg/kg Cr. The supplementation of 500 mug/kg Cr increased body weight, weight gain and feed efficiency (p broiler chicken and can be used as additives in animal diet but it still needs more investigations.

  19. EFFECT OF DIETARY TRYPTOPHAN LEVELS ON GROWTH PERFORMANCE OF BROILER CHICKENS REARED IN THE HOT SEASON UNDER TROPICAL ENVIRONMENT

    Directory of Open Access Journals (Sweden)

    Emmanuel Opoola

    2017-12-01

    Full Text Available This study was conducted to evaluate the effect of dietary levels of tryptophan on growth performance of broiler chickens reared under tropical environment. At the starter phase, a total of two hundred and eighty five day old mixed sex Arbor Acres broiler chicks were randomly allotted to five dietary treatments with three replicates each having nineteen (19 chicks. At the finisher phase, two hundred and seventy broilers were also allotted to five dietary treatments with three replicates each having 18 broilers per replicate. The dietary tryptophan levels at the starter phase were 0.15, 0.19, 0.23, 0.27 and 0.31% respectively while the diets for the finisher phase contained 0.13, 0.17, 0.21, 0.25 and 0.29% dietary tryptophan respectively. All other nutrient levels were constant. The experiment was conducted at 0 to 28d (starter phase and 33 to 56d (finisher phase. Growth performance traits including weight gain, feed intake and feed conversion ratio were recorded at the end of each week. The results for the starter phase showed that chicks fed diet containing 0.23%, 0.27% and 0.31% dietary tryptophan had similar results in term of the weight gain, average daily weight gain, feed intake and average daily feed intake. For the finisher phase, the birds fed 0.21%, 0.25% and 0.29% tryptophan diets also had similar results in terms of final weight, weight gain, feed intake and average daily feed intake. Our results suggest that supplemental tryptophan was sufficient to have significant (P<0.05 effect on broiler performance. However, polynomial regression analysis reveals that the optimum performances were reached at 0.24% and 0.21% dietary tryptophan for the starter and finisher phases respectively. Therefore, it can be concluded that dietary tryptophan requirements during the hot season for the starter and finisher phases were 0.24% and 0.21%, respectively.

  20. Experimental rickets in broilers: gross, microscopic, and radiographic lesions. II. Calcium deficiency

    International Nuclear Information System (INIS)

    Long, P.H.; Lee, S.R.; Rowland, G.N.; Britton, W.M.

    1984-01-01

    Day-old broiler chicks were fed a calcium-deficient diet for 30 days. Gross, microscopic, and radiographic examinations of the proximal tibiotarsus were done at 2, 3, and 4 weeks of age. In 2-week-old chicks, the growth-plate proliferating-prehypertrophied zone (P-PHZ) was variably lengthened and disorganized, cartilage columns of the degenerating hypertrophied zone and metaphyseal primary spongiosa were shortened, and bone spicules of the secondary spongiosa were bordered by increased osteoid. In 3-week-old chicks, lesions were either similar or more pronounced, with the additional finding of metaphyseal peritrabecular fibrosis. In 4-week-old chicks, the P-PHZ decreased in relative length, osteoid seams and fibrous connective tissue were less prominent, and tibial dyschondroplasia-like lesions were present. Results demonstrated that dietary calcium deficiency produced rachitic lesions, that the lesions were very different from those of phosphorus deficiency or calcium excess, and that the lesions were variable over time. (author)

  1. Effects of different levels of dietary crude protein and threonine on performance, humoral immune responses and intestinal morphology of broiler chicks

    Directory of Open Access Journals (Sweden)

    MA Abbasi

    2014-03-01

    Full Text Available The present study aimed at investigating the effects of different dietary crude protein (CP and threonine (Thr levels on the performance, immune responses and jejunal morphology of broiler chicks. A total of 432 broiler chicks were randomly assigned to a 3×3 factorial arrangement of treatments including three different CP dietary levels (90, 95, and 100% of Ross 308 recommendations and Thr (100, 110, and 120% of Ross specifications dietary levels. Performance parameters were recorded for the starter (1-12 days, grower (13-24 days and finisher (25-42 days periods. Birds were subjected to different antigen inoculations to evaluate antibody responses. At day 42 of age, two randomly-selected birds per replicate were slaughtered to measure carcass traits. Although Thr dietary supplementation had no marked effect on Newcastle antibody titers, particularly the supplementation of Thr up to 110% of Ross specifications improved (p<0.05 antibody titers against sheep red blood cells during both primary and secondary responses. Reduction of dietary CP level resulted in significant decrease in villus height (p<0.05 and crypt depth (p<0.01 in jejunal epithelial cells, but the supplementation of low-CP diets with Thr up to 110 and 120% of the recommended values allowed overcoming these changes. Except for the starter period, reducing dietary CP level to 90% of Ross recommendations had no harmful effects on performance parameters; however, the best values were obtained with diets containing 110% Thr. The present results indicate that it is possible to reduce dietary CP level up to 10% after the starter period without any detrimental impact on growth performance, and dietary Thr supplementation up to 110% of Ross values may compensate for low CP-induced growth delay in broiler chicks.

  2. Performance of broilers fed increased levels energy in the pre-starter diet and on subsequent feeding programs having with acidulated soybean soapstock supplementation

    Directory of Open Access Journals (Sweden)

    SL Vieira

    2006-03-01

    Full Text Available This study aimed to evaluate broiler responses to increases in feed energy (2,870, 3,000 and 3,100 kcal ME/kg and the inclusion of Acidulated Soybean Soapstock (ASS when compared to Degummed Soybean Oil (DSO in feeds from placement to 7 days of age. From 7 to 42 days ASS or DSO were included in diets that contained similar energy and nutrient levels. Metabolizable energy values used to formulate the diets for ASS and DSO were 8,351 and 7,701 kcal ME/kg in the first week and 9,314 and 8,559 kcal ME/kg afterwards, respectively. Diets were based on corn and soybean meal and were fed to 1,600 one-d-old male broiler chicks randomly placed in 40 floor pens. No differences in performance due to fat source were seen at 7 days. However, the increase in energy levels to 3,100 kcal ME/kg reduced feed intake, whereas feed conversion was improved with energy at 3,000 kcal ME/kg. Live performance, and the yields of carcass and commercial cuts were not affected by the type of fat included in the feeds from 7 to 42 days, except for increased body weight at 21 and 35 days with ASS supplementation. Litter moisture at 7, 21, 35 and 42 days was not affected by any of the factors and there were no residual effects of treatments at 21, 35 and 42 days of age. On the other hand, body weight at 35 days was affected by the interaction of diets fed in the first week with those provided afterwards. The results showed that ME values used for DSO and ASS are adequate and that ASS may be used as fat source in broiler feeds from placement to 42 days of age.

  3. Residues of dioxins and PCBs in fat of growing pigs and broilers fed contaminated feed

    NARCIS (Netherlands)

    Hoogenboom, L.A.P.; Kan, C.A.; Bovee, T.F.H.; Weg, van der G.; Onstenk, C.G.M.; Traag, W.A.

    2004-01-01

    To investigate the kinetics of PCBs and dioxins, 3 week old broilers and 3 month old pigs were fed with a 10-fold diluted feed from the Belgium crisis for one week, followed by a period on clean feed. In the case of broilers this resulted in levels for dioxins, non-ortho and mono-ortho PCBs in fat

  4. Influence of different yeast cell wall preparations and their components on performance and immune and metabolic pathways in Clostridium perfringens-challenged broiler chicks

    Science.gov (United States)

    A study was conducted to evaluate the influence of purification of yeast cell wall (YCW) preparations on broiler performance, and immunogenic and metabolic pathways under microbial challenge. A total of 240 day-of-hatch chicks were distributed among two battery brooder units (48 pens; 5 birds/pen; ...

  5. Performance and organ morphology of broilers fed microbial or antimicrobial additives and raised in batteries or floor pens

    Directory of Open Access Journals (Sweden)

    Pedroso AA

    2003-01-01

    Full Text Available This study was conducted to determine the effect of microbial or antimicrobial additives on the performance and organ morphology of broilers raised in batteries or in floor pens. The effect of microbial additives on the presence of oocysts in the litter was also studied. Experiments 1 and 2 consisted of four treatments (non-supplemented control diet or diet supplemented with avilamycin, bacitracin methylene disalicylate or enramycin and six repetitions in a randomized block design. In Experiment 1, 288 day-old chicks were housed in heated batteries in a environmentally controlled room, 12 chicks per cage; in Experiment 2, 1,200 day-old chicks were housed in a curtain-sided experimental house, with concrete floor and rice hulls as litter material, 50 chicks per pen. Experiments 3 and 4 were carried out similarly to Experiments 1 and 2, respectively, but the treatments consisted of microbial additives (non-supplemented control diet or Bacillus subtilis added to the feed plus Lactobacillus reuteri and Lactobacillus johnsonii added to the water, undefined microflora added to the water or live yeast added to the feed. The antibiotics did not affect the performance of birds raised in batteries, but improved feed conversion, weight gain and live weight when chickens were kept on the floor pens. Microbial additives did not affect bird performance in any environment; however, treatments affected liver weight. Microbial agents increased intestinal weight in floor-raised broilers. No relationship was seen between the use of microbial additives and the presence of oocysts in the litter.

  6. Effect of Andrographis paniculata and Psidium guajava leaves on growth performance and carcass of broiler chicken

    Directory of Open Access Journals (Sweden)

    Watanasit, S.

    2005-08-01

    Full Text Available A six-week experiment was conducted to study the effects of Andrographis paniculata (AP and Psidium guajava (PG on the growth performance and carcass quality of broiler chickens. Six hundred one-day-old (mixed sex broiler chicks were used in ten dietary treatments, in a completely randomized design experiment. There were three replications in each treatment with 20 chicks per pen. The dietary treatments were 1 basal diet (control, 2 basal diet + antibiotic, 3 basal diet + 0.2% AP, 4 basal diet + 0.4% AP, 5 basal diet + 0.2% PG, 6 basal diet + 0.4% PG, 7 basal diet + 0.2% AP + 0.2% PG, 8 basal diet + 0.2% AP + 0.4% PG, 9 basal diet + 0.4% AP + 0.2% PG and 10 basal diet + 0.4% AP + 0.4% PG From 0-3, 3-6 and 0-6 weeks, feed intake, body weight gain, feed conversion ratio and mortality rate were not significantly different (P>0.05 among treatments. There was no significant difference (P>0.05 in percentages of eviscerated carcass, breast and leg among chicken fed different diets However, percentage of abdominal fat of male chicken fed basal diet + 0.2% AP + 0.2% PG were lower (P<0.05 than other treatments while female chickens fed the same diet were not statistically different.

  7. The performance of broiler finisher birds fed varying levels of feather ...

    African Journals Online (AJOL)

    The performance of broiler finisher birds fed varying levels of feather meal as replacement for soya bean meal. ... meal increased, feed cost/ kg weight gain increased and both differed significantly (P<0.05) between treatment means, while the birds tolerated feather meal up to 7.5% inclusion level, 2.5% was the optimal.

  8. Effect of corn replacement with graded levels of wheat screening and enzyme supplementation on performance, blood lipids, viscosity and jejunal histomorphology of finisher broilers

    Energy Technology Data Exchange (ETDEWEB)

    Mazhari, M.; Golian, A.; Kermanshahi, H.

    2015-07-01

    An experiment was carried out to study the effect of corn replacement with five levels of wheat screening (0, 150, 300, 450 and 600 g/kg of diet) with (0.5 g/kg of diet) or without xylanase-glucanase enzyme on performance, blood lipids, viscosity and jejunal histomorphology of finisher broilers (25-42 days of age). Five hundred day-old Ross-308 male broiler chicks were fed by a standard commercial diet up to 24 days of age, then randomly assigned to 10 diets. Each diet was fed to five groups of ten chicks each. There was not significant differences in body weight gain (BWG), feed intake, and feed conversion ratio of birds fed with different levels of wheat screening (WS), whereas enzyme increased (p<0.05) BWG. Different levels of WS and enzyme did not have a significant effect on relative weights of carcass, breast, thigh, and abdominal fat of broilers. Relative weights of gizzard, pancreas, small and large intestine, and relative length of jejunum and jejunal and ileal viscosity were increased (p<0.05) by WS, while were decreased (p<0.05) by enzyme. The serum cholesterol level decreased (p<0.05) by increasing levels of WS. Jejunal histomorphological observations showed (p<0.05) shorter and thicker villus and lower crypt depth by increasing levels of WS, while addition of enzyme to the diets, affected (p<0.05) reversely to these parameters. The results showed that the addition of wheat screening up to an inclusion level of 600 g/kg of diet had no adverse effect on broiler performance in the finisher (25-42 d) phases whereas decreased serum cholesterol levels, increased viscosity and villus atrophy. The dietary administration of exogenous enzyme improved performance parameters and decreased viscosity and villus atrophy of broiler jejunum. (Author)

  9. Different Levels of Digestible Methionine on Performance of Broiler Starter

    Directory of Open Access Journals (Sweden)

    WL Bryden

    2010-01-01

    Full Text Available Dietary protein and amino acid supply is the most expensive component of poultry diets. Therefore several efforts made by the industry to minimize the cost of the protein portion of the diet. Accordingly, there has been a recent move to use digestible amino acid values in the formulation of poultry diets. The efficiency of protein utilization depends to a large extent on the amino acid composition of the diet. The study was conducted to determine the digestible methionine requirement of broilers during the starter periods. One hundred and seventy five (175 chicks were allocated to 5 treatments with five replicates of seven chicks per replicate in a completely randomized design. Chicks were fed experimental diets from one day old to 21 days of age. Dietary treatments included 5 titrated levels each of digestible methionine (3.0, 4.5, 6.0, 7.5, and 9.0 g/kg diet added to a basal diet. The allowance of digestible methionine, rather than digestible sulphur amino acids was used in formulating the diets. Supplemental synthetic DL-Methionine which were considered to be 100% digestible were added to diets to obtain the concentration of the digestible amino acid. Each week until the conclusion of the trial, birds were individually weighed, feed intake per pen was measured, and feed conversion ratio (FCR was computed. This study suggested that the digestible methionine requirement for broiler starter is 4.7 g/kg for optimal body weight gain and 4.6 g/kg for optimal feed conversion ratio. (Animal Production 12(1: 6-11 (2010Key Words: amino acid, broiler, digestible, methionine, starter

  10. Influence of Phytase Transgenic Corn on the Intestinal Microflora and the Fate of Transgenic DNA and Protein in Digesta and Tissues of Broilers

    Science.gov (United States)

    Li, Sufen; Li, Ang; Zhang, Liyang; Liu, Zhenhua; Luo, Xugang

    2015-01-01

    An experiment was conducted to investigate the effect of phytase transgenic corn (PTC) on intestinal microflora, and the fate of transgenic DNA and protein in the digesta and tissues of broilers. A total of 160 1-day-old Arbor Acres commercial male broilers were randomly assigned to 20 cages (8 chicks per cage) with 10 cages (replicates) for each treatment. Birds were fed with a diet containing either PTC (54.0% during 1–21 days and 61.0% during 22–42 days) or non-transgenic isogenic control corn (CC) for a duration of 42 days. There were no significant differences (P>0.05) between birds fed with the PTC diets and those fed with the CC diets in the quantities of aerobic bacteria, anaerobic bacteria, colibacillus and lactobacilli, or microbial diversities in the contents of ileum and cecum. Transgenic phyA2 DNA was not detected, but phyA2 protein was detected in the digesta of duodenum and jejunum of broilers fed with the PTC diets. Both transgenic phyA2 DNA and protein fragments were not found in the digesta of the ileum and rectum, heart, liver, kidney, and breast or thigh muscles of broilers fed with the PTC diets. It was concluded that PTC had no adverse effect on the quantity and diversity of gut microorganisms; Transgenic phyA2 DNA or protein was rapidly degraded in the intestinal tract and was not transferred to the tissues of broilers. PMID:26599444

  11. Comparative Study of Ashwagandha and Commercial Synthetic Compound on Performance of Broilers during Hot Weather

    Directory of Open Access Journals (Sweden)

    J. M. Shisodiya

    Full Text Available The trial was conducted for a period of six weeks on 225 day old broiler chicks, uniformly distributed into four groups of 75 chicks in each T1, T2 and T3 group. The chicks were fed with standard starter mash which contained crude protein 22.84% and metabolizable energy 2852.5 Kcal / kg (calculated value up to three weeks of age. For next 3 weeks i.e. from 4 to 6 weeks of age with finisher mash which contained crude protein 20% and metabolizable energy 2966 Kcal / kg (calculated value. Group T1 received standard broiler diet. Group T2 and T3 received standard broiler diet supplemented with Ashwagandha and commercial synthetic compound @ 0.05 % of feed respectively. The experimental birds were reared on deep litter system and rice husk was used as litter material.The supplementation of Aswagandha and commercial synthetic compound recorded significant improvement in all studied growth parameter i.e. live body weights, weekly gain in body weights and feed conversion ratio was observed in all the supplemented groups over the control group. However, feed consumption in control group was significantly higher than supplemented group. The economic returns of supplemented groups are more than the unsupplemented group. The net profit per bird was maximum in the commercial synthetic compound supplemented group followed by Ashwagandha supplemented group and lowest was recorded in control group (T1. [Veterinary World 2008; 1(10.000: 310-311

  12. Effect of Incubator Type and Broiler Breeder Age on Hatchability and Chick Quality

    Directory of Open Access Journals (Sweden)

    ICS Araújo

    Full Text Available ABSTRACT Two experiments were conducted to evaluate the effect of broiler breeder age and incubator type on hatching parameters, hatch window, embryo diagnosis results, and hatchling physical quality. The treatments consisted of a combination of three broiler breeder ages (29, 35 and 59 weeks of age and two incubator types (single stage, SS; or and multiple stage, MS. A completely randomized design in a 3x2 factorial arrangement was applied. In Experiment I, 1,896 eggs were used and 360 eggs in Experiment II. There was an interaction between breeder age and incubator type only for hatchling physical quality score. Independently of incubator type, hatchability rate, late embryo mortality, and egg contamination were higher in the eggs laid by older breeders (59-wk-old. Early mortality (0-4 days was higher in the embryos from young breeders (29-wk-old. A shorter hatch window birth was obtained in the SS incubator, resulting in higher hatchling body weight relative to egg weight, and better hatchling physical quality score. Both types of incubators provide good conditions for embryo development; however, the physical quality of chicks derived from eggs from intermediate-aged breeders (35-wk-old is better when eggs are incubated in SS incubators.

  13. Vaccination of commercial broiler chicks against avian metapneumovirus infection: a comparison of drinking-water, spray and oculo-oral delivery methods.

    Science.gov (United States)

    Ganapathy, Kannan; Bufton, Andrew; Pearson, Andrew; Lemiere, Stephane; Jones, Richard C

    2010-05-21

    Avian metapneumovirus (aMPV) has become an important cause of viral respiratory infections in turkey and chickens. Live and inactivated vaccinations are available worldwide for prevention of disease and economic losses caused by this pathogen. The efficacy of these vaccines is vigorously tested under laboratory conditions prior to use in the field. In this study, a live subtype B aMPV vaccine was administered by spray, drinking water or oculo-oral methods to separate groups of broiler chicks under field conditions. Following this, the chicks were immediately transferred to separate rooms in an experimental isolation house, monitored and challenged with virulent subtype B aMPV. No clinical signs were recorded following the vaccination methods. In the oculo-oral vaccinated chicks, 40-60% of the birds were vaccine virus positive by RT-PCR. In addition, in comparison to other groups, statistically higher levels of aMPV ELISA antibodies were detected. After spray vaccination, the number of chicks positive for the vaccine virus increased gradually from 10% at one week to 30% by 3 weeks post vaccination. Following drinking water vaccination, 30% of chicks were aMPV positive at 1 week but negative by 3 weeks post vaccination. In both, spray and drinking water vaccinated groups, no ELISA antibodies were detected, but when challenged all chicks were protected against disease. At 5 days post challenge, 100% of chicks in the unvaccinated and those vaccinated by spray or drinking water routes but only 20% of the oculo-oral-vaccinated chicks were aMPV positive by RT-PCR. At 10 days post challenge, 10% of chicks in each group were aMPV RT-PCR positive. On challenge, all vaccinated chicks were protected against disease. It appears that when aMPV vaccine is accurately applied to chicks by spray or drinking water routes, both are capable of giving protection against clinical disease equal to that induced in those chicks vaccinated individually by the oculo-oral route. Copyright 2010

  14. Bacillus subtilis-based direct-fed microbials augment macrophage function in broiler chickens

    Science.gov (United States)

    The present study was conducted to evaluate the function of Bacillus subtilis-based direct-fed microbials (DFMs) on macrophage functions, i.e., nitric oxide (NO) production and phagocytosis in broiler chickens. DFMs used in this study were eight single strains designated as Bs2084, LSSAO1, 3AP4, Bs1...

  15. Dietary oregano essential oil alleviates experimentally induced coccidiosis in broilers.

    Science.gov (United States)

    Mohiti-Asli, M; Ghanaatparast-Rashti, M

    2015-06-15

    An experiment was conducted to determine the effects of oregano essential oil on growth performance and coccidiosis prevention in mild challenged broilers. A total of 250 1-d-old chicks were used in a completely randomized design with 5 treatments and 5 replicates with 10 birds in each replication. Experimental treatments included: (1) negative control (NC; unchallenged), (2) positive control (PC; challenged with sporulated oocysts of Eimeria), (3) PC fed 200 ppm Diclazuril in diet, (4) PC fed 300 ppm oregano oil in diet, and (5) PC fed 500 ppm oregano oil in diet. At 22 d of age, all the experimental groups except for NC were challenged with 50-fold dose of Livacox T as a trivalent live attenuated coccidiosis vaccine. On d 28, two birds were slaughtered and intestinal coccidiosis lesions were scored 0-4. Moreover, dropping was scored in the scale of 0-3, and oocysts per gram feces (OPG) were measured. Oregano oil at either supplementation rate increased body weight gain (P=0.039) and improved feed conversion ratio (P=0.010) from d 22 to 28, when compared with PC group. Using 500 ppm oregano oil in challenged broilers diet increased European efficiency factor than PC group (P=0.020). Moreover, challenged broilers fed 500 ppm oregano oil or Diclazuril in diets displayed lower coccidiosis lesions scores in upper (P=0.003) and middle (P=0.018) regions of intestine than PC group, with the effect being similar to unchallenged birds. In general, challenged birds fed 500 ppm oregano oil or Diclazuril in diets had lower OPG (P=0.001), dropping scores (P=0.001), litter scores (P=0.001), and pH of litter (P=0.001) than PC group. It could be concluded that supplementation of oregano oil at the dose of 500 ppm in diet may have beneficial effect on prevention of coccidiosis in broilers. Copyright © 2015 Elsevier B.V. All rights reserved.

  16. Infectious bursal disease virus: case report and experimental studies in vaccinated and unvaccinated SPF chickens and commercial broiler chicks

    Directory of Open Access Journals (Sweden)

    H Scanavini Neto

    2004-03-01

    Full Text Available IBDV Gm 11 (Simbios eleven-molecular group has been detected since 1997 in many farms of commercial broilers and layers causing high mortality (2 to 15% and severe macro and microscopic damage in cloacal bursae, spleen, thymus, kidney and liver. Five serial passages of 2050/97-Gm 11 IBDV sample by CAM route in SPF chicken's embryonated eggs did not elicit increased embryo mortality. High mortality (100% of 21 day-old SPF leghorn chickens and severe bursal and splenic lesions were seen from 24 up to 48 hours after eye-drop inoculation of 2050/97 strain (50 mL of 10-2 dilution of 10% bursae homogenate. Mortality was not detected when vaccinated SPF and broiler chickens were inoculated. One dead bird was found among ten challenged unvaccinated broilers. Variations in the intensity of cloacal bursae injury and spleen response were found between unvaccinated and vaccinated broiler chickens. IBDV antibodies were detected by ELISA test in almost all vaccinated SPF chickens before challenge while low number of commercial vaccinated and unvaccinated broilers were serologically positive (0 to 3 birds in 18. Increasing IBDV antibody titers were detected after challenge with 2050/97 strain and highest GMTs were found in broilers. It was concluded that 2050/97 strain is a highly virulent IBDV and SPF leghorn chickens immunized with BV8 intermediate vaccine strain were resistant to the challenge. Increasing susceptibility was found from experimental groups of unvaccinated broilers to vaccinated broilers and to unvaccinated SPF birds. It is discussed that passive immunity was involved in the rate of protection of challenged unvaccinated broiler and in the immune response impairment after vaccination of broilers chicks. The use of a constant virus suspension with known potency to challenge the experimental birds was suitable to evaluate vaccination efficacy. Evaluation of bursal and splenic responses at early and delayed time after challenge were useful to

  17. Energy utilisation of broiler chickens in response to guanidinoacetic acid supplementation in diets with various energy contents.

    Science.gov (United States)

    Ale Saheb Fosoul, Sayed Sadra; Azarfar, Arash; Gheisari, Abbasali; Khosravinia, Heshmatollah

    2018-07-01

    This experiment was conducted to investigate the effects of guanidinoacetic acid (GAA) on productive performance, intestinal morphometric features, blood parameters and energy utilisation in broiler chickens. A total of 390 male broiler chicks (Ross 308) were assigned to six dietary treatments based on a factorial arrangement (2×3) across 1-15 and 15-35-d periods. Experimental treatments consisted of two basal diets with standard (STD; starter: 12·56 MJ/kg and grower: 12·97 MJ/kg) and reduction (LME; starter: 11·93 MJ/kg and grower: 12·33 MJ/kg) of apparent metabolisable energy (AME) requirement of broiler chickens each supplemented with 0, 0·6 and 1·2 g/kg GAA. Supplemental 1·2 g/kg GAA decreased the negative effects of feed energy reduction on weight gain across starter, growing and the entire production phases (PEnergy retention as fat and total energy retention were increased when birds received LME diets supplemented with 1·2 g/kg GAA (Penergy for production (NEp) and total heat production increased in birds fed LME diets containing 1·2 g/kg GAA (P<0·05). A significant correlation was observed between dietary NEp and weight gain of broilers (r 0·493; P=0·0055), whereas this relationship was not seen with AME. Jejunal villus height and crypt depth were lower in birds fed LME diets (P<0·05). Serum concentration of creatinine increased in broilers fed LME diets either supplemented with 1·2 g/kg GAA or without GAA supplementation (P<0·05). Supplemental GAA improved performance of chickens fed LME diet possibly through enhanced dietary NEp. The NEp could be preferred over the AME to assess response of broiler chickens to dietary GAA supplementation.

  18. Studies on the efficacy of vitamin c and saccharomyces cerevisiae in providing partial protection against ochratoxicosis in broiler chickens

    International Nuclear Information System (INIS)

    Hatab, M.H.M.

    2011-01-01

    This study was carried out at the experimental animal house and laboratory belonging to poultry physiology unit, Biological Application Department, Atomic Energy Authority, Egypt from November to December(2008). The aim of this work was to investigate the effects of supplementing broiler diets with active dried yeast; Saccharomyces cerevisiae (Sc), vitamin C (Vit.C) or their combination to alleviate the deleterious effect of ochratoxin A (OA) on productive performance, weights, functional and histological profile of internal organ and physiological and immunological parameters. A total number of 368 sexed male Ross broiler chicks were used in this study. The chicks were randomly distributed according to diet supplementation into eight groups (46 chicks each) from 1 day old to 5 weeks of age. The results obtained showed that presence OA at 200 ppb in broiler diets caused significant decrease in body weight, feed consumption and worse feed conversion ratio, hemoglobin, packed cell volume of blood, red and white blood cell counts, blood proteins, thyroid hormones, immune response and weights of thymus and bursa and alteration in histological picture of kidney, liver and bursa. At the same time, a significant increase in relative weights of kidney, liver, gizzard, proventriculus, AST, ALT enzymes, uric acid, creatinine in addition to high mortality percentage. Addition Sc either alone or in combination with vit.C to OA- fed groups alleviated the toxic effects of OA on productive performance, immunological, hematological, biochemical and histological parameters. On the other hand, the addition of vit.C to OA- contaminated broiler diet did not prevent the negative effects of OA observed in chicks, except for some measurements as body weight, mortality percentage and blood proteins, although less extent was shown compared to the Sc or Sc + vit.C supplementation.

  19. Multiple-enzyme supplementation on digestive traits, carcass characteristics, blood lipid parameters and growth performance of broilers fed a wheat-based diet

    Directory of Open Access Journals (Sweden)

    Hamid Reza Taheri

    2017-09-01

    Full Text Available Objective A trial was conducted from 11 to 42 d post-hatch to investigate the effectiveness of the supplementation of a multiple-enzyme preparation (Natuzyme Plus in a wheat-based diet on digesta viscosity, pH and microbial population, villus morphology, feed passage time, nutrient retention, carcass characteristics, blood lipid parameters and growth performance of broiler chickens. Methods Three hundreds 10-d-old male Ross 308 chicks were allocated to three diets with five replicates of 20 birds per replicate. Dietary treatments were i a wheat-based diet (W, ii W+Natuzyme Plus (WN; 500 mg/kg of the diet, and iii a corn-based diet (C. Results Birds fed on the C diet had higher average daily gain (ADG, p0.05 difference compared to those of the C diet. Compared to those of the W diet, the WN diet showed the higher count of Lactobacilli and lower count of coliforms (p<0.01 and digesta viscosity (p<0.01. Conclusion In general, the results of this study showed that Natuzyme Plus supplementation in a wheat-based diet can be appropriate to achieve a comparable growth performance in broiler chickens to those given the C diet probably through improving digesta viscosity, VH, ET, TTAR of NT and EE, AMEn, count of Lactobacilli and coliforms.

  20. MIGRATION OF PHTHALATES FROM PLASTIC TANK TO VEGETABLE OIL AS A PART OF FEEDING MIXTURES USED FOR CHICKEN BROILERS FATTENING

    Directory of Open Access Journals (Sweden)

    Pavel Suchý

    2010-05-01

    Full Text Available The concentrations of phthalic acid esters (PAEs as di-n-butyl phthalate (DBP and di-2-ethylhexyl phthalate (DEHP were measured in samples of rapeseed oil, which was used as a feed. First samples were collected during the production process and second after the storage in plastic tank (21 days. The results of measurements are that there is 2.93-10.10 mg PAEs.kg-1 in the oil before storage and 22.73-61.55 mg PAEs.kg-1 after storage. For the monitoring of distribution and accumulation of PAEs in animal tissues and organs (muscles, adipose tissue, skin and liver broiler chicks ROSS 308 were used. The chicks were divided into 4 groups (50 chicks each. All the chicks were fed by commercial diets (complete feed, KKS for broiler chicks (starter – BR1; grower – BR2 and finisher – BR3. The experimental diets were supplemented with vegetable oil (RO with low (group N or high (group V phthalate content, or animal fat with high phthalate content (group Z. Neither the control diets (K nor the grower (BR1 diets contained vegetable oil or animal fat. DBP and DEHP were found in all tissues of all chicks. The highest concentration of DBP of 1.28 1.00 mg.kg-1 of fresh sample (an average value from 8 chicks was determined in the adipose tissue of V chicks. The highest concentration of DEHP of 3.27 2.87 mg.kg-1 of fresh sample (average of 8 chicks was also determined in the V group. doi:10.5219/49

  1. Addition of Amylase from Aspergillus Awamori to the Diet of Broiler Chickens

    Directory of Open Access Journals (Sweden)

    HS Morgado

    Full Text Available ABSTRACT Two experiments were performed to evaluate the hematological and blood biochemistry parameters, biometry of digestive organs, enzyme activities, protein content and absolute weight of the pancreas of broilers fed pre-starter and pre-starter diets supplemented or not with amylase from Aspergillus awamori. In total, 120 male Cobb chicks were housed in heated cages in each experiment. A completely randomized experimental design, with two treatments (feed with and without amylase and six replicates per treatment of 10 birds each was applied. The data were subjected to analysis of variance using the F-test at 5% probability level. The dietary amylase addition did not affect hematological and blood biochemistry parameters and the biometry of the gastrointestinal tract of 7- and 21-d-old broilers, nor the absolute weight, enzyme activities or protein concentration of the pancreas of 7-d-old broilers. However, the inclusion of amylase in the diet reduced amylase activity and pancreatic protein concentration in 21-d-old broilers. The application of amylase to broiler chicken pre-starter and starter feeds is not justified given the pancreatic amylase activity and protein concentrations.

  2. Vom work Book Journal 2011, 2010 4th Edition PDF

    African Journals Online (AJOL)

    USER

    twenty four, 2-day old, broiler chicks in three replicates of 8 chicks and feeding lasted ... Performance and Nutrient Retention of Broilers fed Treated. Pumpkin Kernel Diets in .... that the two diets were higher in caloric density than the groundnut ...

  3. Hematological alterations in broiler chicks during different seasons supplemented with herbal formulations

    Directory of Open Access Journals (Sweden)

    Nidhi Singh1

    2008-08-01

    Full Text Available The study was conducted on Vencobb broiler chicks to ascertain the antistress affects of Zist, Zeetress and a combination of Amla and Turmeric during summer, rainy and winter seasons and thereby the haematological changes of birds revealed that during all the three seasons a marked improvement in Haemoglobin was encountered in all the three groups where herbal preparations were supplemented. A marked increase in the lymphocyte count occurred during summer and winter seasons in all the experimental groups of bird as compared to the control group. The heterophil count was decreased with the supplementation of herbal formulations in the feed irrespective of the seasons encountered in the season. This decrease in heterophil count was highly significant in seasons like summer, winter and rainy. This observation proves the hepato-stimulatory, hepato protective and immuno modulating effects of herbal preparations. [Veterinary World 2008; 1(4.000: 110-112

  4. Effects of ground thyme and probiotic supplements in diets on broiler performance, blood biochemistry and immunological response to sheep red blood cells

    Directory of Open Access Journals (Sweden)

    Seyed A. Hosseini

    2013-03-01

    Full Text Available A trial was conducted to study the effects of the aromatic plant thyme, a commercial probiotic (Protexin and avilamycin on broiler performance, blood biochemical parameters and also the antibody response to sheep red blood cells. A total of 750 broilers were assigned into five replicate groups for each of five dietary treatments, namely; control (C, 2.5 mg/kg avilamycin (AB, 0.1 g/kg commercial probiotic (P, 5 g/kg ground thyme (T1, and 7.5 g/kg ground thyme (T2. In general, body weight, feed consumption and feed conversion ratio were not affected by dietary treatments compared to the control birds (P>0.05. Birds fed the P supplemented treatment had the greatest serum protein levels (P<0.001 and highest albumin levels (P<0.001 when compared with control birds, while the birds fed T2 had the lowest (P<0.001. Dietary supplementation reduced (P<0.001 cholesterol and triglyceride concentration in serum of broilers, with the effect were more noticeable by P supplements. Moreover, blood calcium and phosphorus concentrations were higher (P<0.001 in birds fed the P-supplemented diet compared to the birds fed the control diets. This study suggests that probiotic supplementation in particular, and to an intermediate extent ground thyme supplementation in diets of broiler, resulted in chicks with favorably improved blood biochemical parameters and mineral utilization, compared to the birds fed diets supplemented with avilamycin or without any supplementation.

  5. Dietary Effect of Selenium-enriched Radish Sprouts, Vitamin E, and Rhodobacter capsulatus on Hypocholesterolemia and Immunity of Broiler

    Directory of Open Access Journals (Sweden)

    Tsujii H

    2017-06-01

    Full Text Available The study was designed to investigate the effects of dietary Selenium-enriched radish sprouts (Se-RS, Vitamin E (Vit E, and Rhodobacter capsulatus (RC on immunity, cholesterol concentration, and fatty acid composition in broiler meat. A total of 100 two-week-old male broiler chicks were randomly assigned into five dietary groups: I Control; II Se-RS (5 μg/kg Se-RS; III Se-RS+RC (5 μg/kg Se-RS + 0.2 g/kg RC; IV Se-RS+Vit E (5 μg/kg Se-RS + 50 mg/kg Vit E and V Se-RS+RC+Vit E (5 μg/kg Se-RS + 0.2 g/kg RC + 50 mg/kg Vit E. Diets and clean drinking water were offered ad libitum. After the end of 3-wk of feeding period, serum cholesterol and triglycerides concentrations were lower (P < 0.05 in broilers fed Se-RS + RC + Vit E supplemented diet compared to the control diet. At the end of the 6-wk feeding period, birds fed the Se-RS+RC+Vit E diet significantly (P < 0.05 reduced cholesterol and triglycerides concentrations and improved the ratio of unsaturated fatty acids to saturated fatty acids in broiler meat. The highest (P < 0.05 number of leukocytes was observed in broilers fed Se-RS+RC+Vit E supplemented diet. Foot web index and weights of spleen, bursa, and thymus were significantly (P < 0.05 higher in birds fed Se-RS+RC+Vit E compared to the control diet. Our findings suggest that there are dual benefits of supplementing broiler diets with Se-RS+RC+Vit E because of improvements in the bird’s immunity and meat quality that is important for health conscious consumers.

  6. Skeletal response to diet with soya bean seeds used as primary source of protein in growing broiler chickens.

    Science.gov (United States)

    Olkowski, B; Charuta, A; Radzki, R; Bieńko, M; Toczko, R

    2016-08-01

    The study was conducted using 120 commercial broiler chicks (Ross 308) randomly allocated to two experimental groups. The experimental diets, differing only in protein source, either solvent-extracted soya bean meal (SBM) or traditional (non-genetically modified) full-fat soya bean seeds (FFS), were prepared using practical corn-based formulation designed to meet nutritional requirements of broilers. Performance parameters were monitored weekly. Also, the subjects were evaluated daily for overt changes in skeletal anatomy and gait physiology. Randomly selected chickens from each group (seven males and seven females) were euthanized at 2, 3, 4 and 6 weeks of age, and bone specimens were collected for further study. Bone mineral density (BMD) and bone mineral content (BMC) were determined in tibiotarsal bones. Broilers fed FFS diet showed retarded growth rate and decreased feed intake (both p chickens from the FFS group in comparison with the SBM group. The chickens fed the FFS diet showed higher incidence of skeletal pathology including angular deformities and torticollis (both p chickens. Journal of Animal Physiology and Animal Nutrition © 2016 Blackwell Verlag GmbH.

  7. The utilization of niacin in broilers

    International Nuclear Information System (INIS)

    Wen Jie; Wang Hemin

    1993-01-01

    The experiment was conducted to investigate the distribution, storage and utilization of niacin in broilers. 125 Arbor Acre broilers were used for two treatments. For Treatment 1, 80 chicks was injected with 20 μCi of 3 H-niacin when they were 15 days old. For treatment 2, 45 chicks were subdivided into 3 groups with injections of 15, 30 and 60 mg/kg niacin diet labeled with 5 μCi 3 H-niacin. Feces were collected 5 ∼ 7 days after injection. The results showed that for three-week-old chicks, the contents of 3 H-niacin in liver and blood dropping to 1/2 of peak value took 19 and 21 hours, and dropping to 1/5 of peak value took 78 and 169 hours, respectively. The supplementation of niacin at the level of 15 ∼ 60 mg/kg diet had no significant effect on niacin availability. The distribution of niacin in chick body was uniform. No true storage organ was found

  8. Effect of feeding soybean meal and differently processed peas on intestinal morphology and functional glucose transport in the small intestine of broilers.

    Science.gov (United States)

    Röhe, I; Boroojeni, F Goodarzi; Zentek, J

    2017-09-01

    Peas are locally grown legumes being rich in protein and starch. However, the broad usage of peas as a feed component in poultry nutrition is limited to anti-nutritional factors, which might impair gut morphology and function. This study investigated the effect of feeding raw or differently processed peas compared with feeding a soybean meal-based control diet (C) on intestinal morphology and nutrient transport in broilers. A total of 360 day-old broiler chicks were fed with one of the following diets: The C diet, and 3 diets containing raw peas (RP), fermented peas (FP) and enzymatically pre-digested peas (EP), each supplying 30% of dietary crude protein. After 35 d, jejunal samples of broilers were taken for analyzing histomorphological parameters, active glucose transport in Ussing chambers and the expression of genes related to glucose absorption, intestinal permeability and cell maturation. Villus length (P = 0.017) and crypt depth (P = 0.009) of EP-fed broilers were shorter compared to birds received C. The villus surface area was larger in broilers fed C compared to those fed with the pea-containing feed (P = 0.005). Glucose transport was higher for broilers fed C in comparison to birds fed with the EP diet (P = 0.044). The sodium-dependent glucose co-transporter 1 (SGLT-1) expression was down-regulated in RP (P = 0.028) and FP (P = 0.015) fed broilers. Correlation analyses show that jejunal villus length negatively correlates with the previously published number of jejunal intraepithelial T cells (P = 0.014) and that jejunal glucose transport was negatively correlated with the occurrence of jejunal intraepithelial leukocytes (P = 0.041). To conclude, the feeding of raw and processed pea containing diets compared to a soybean based diet reduced the jejunal mucosal surface area of broilers, which on average was accompanied by lower glucose transport capacities. These morphological and functional alterations were associated with observed mucosal immune

  9. A Comparative Assessment of the Broiler:Corn Ratio and Its Impact on Broiler Processors' Profitability

    OpenAIRE

    Hamm, Sandra J.; Goodwin, Harold L., Jr.; McKenzie, Andrew M.

    2008-01-01

    Input prices for broiler production, particularly corn, are becoming increasingly volatile due to increasing competition for corn from ethanol and biofuels production suggesting volatility in poultry profits will follow indicator of profits relating feed input prices and broiler meat output prices, such as a Broiler:corn ratios. Total chicken exports, total chicken ready-to-cook production, number of eggs set, number of chicks placed, and cold storage chicken inventory are used to estimate. U...

  10. Immune modulation by Bacillus subtilus-based direct-fed microbials in commercial broiler chickens

    Science.gov (United States)

    Direct-fed microbials (DFMs), also known as probiotics, have been successfully used to improve the balance of gut microbiota. Spores of Bacillus subtilis, have been used as DFMs for food animals and humans and our previous studies showed that dietary supplementation of broiler chickens with a B. su...

  11. Comparison of antibody titer against the infectious bursal disease virus following the disease with that obtained from live intermediate vaccines using indirect hemagglutination (IHA test in broiler chicks

    Directory of Open Access Journals (Sweden)

    A Feizi

    2009-02-01

    Full Text Available In this study, antibody titer obtained from the outbreak of the infection bursal disease (IBD was compared with the titer obtained from live intermediate vaccines by indirect haemagglutination (IHA test in broiler chicks. A total of 450 one day old Cobb chicks were divided into 3 groups each containing 150 chicks and were kept for 42 days in the same rearing conditions. Chicks in groups 1 and 2 received Bursin-2 and D-78 vaccines respectively via drinking water n days 14 and 21. The rest of the chicks were kept as the controls (group 4 and did not receive any vaccine against the IBD. Serum samples were collected from all birds 2 weeks after the second IBD vaccination. Additional 150 serum samples were also collected from 3 broiler flocks that were affected by IBD and had a history of vaccination by the previously mentioned method, two weeks after the last clinical sings were observed (group 3. Antibody titer of the samples against the IBD virus were determined by the IHA test and the results were evaluated using ANOVA and SPSS software. The mean antibody titer obtained from Bursin-2 and D-78 vaccines were 3.19 and 3.21 respectively which is less than the titer of 6 needed for protection against the disease. The antibody titer in affected flocks was 7.19. comparison of the mean titer of the two vaccines did not show any significant difference but there was significant difference between the titer obtained from each vaccine and that of the effected flock (p

  12. Effect of immobilized fungal phytase on growth performance and bone traits of broilers fed with low dietary calcium and phosphorus

    Directory of Open Access Journals (Sweden)

    Sreeja Ajith

    2018-06-01

    Full Text Available Aim: The aim of this study was to investigate the effects of phytase which was laboratory produced by Aspergillus foetidus on the growth performance, mineral retention, and bone traits of broilers fed with low dietary calcium and phosphorus. Materials and Methods: The extracellular phytase enzyme secreted into the crude filtrate was concentrated by ammonium sulfate precipitation to obtain an activity of 500 phytase units (FTU. A total of 90 1-day-old chicks (Cobb 500 were randomly divided into three treatment groups with five replicates having six birds each. Dietary treatment, T1, was with 0.45% non-phytate P (NPP during starter and 0.40% during finisher phase with 1% Ca. Dietary treatment, T2, had 0.37% NPP during starter and 0.32% in finisher phase with 1% Ca and supplemental lab phytase at 500 FTU/kg. Dietary treatment, T3, was similar to T2 with a lower Ca of 0.8%. Results: There was no significant difference among the dietary treatments with regard to body weight gain, feed intake, feed conversion ratio, and Ca retention (p>0.05. However, a significant improvement in retention of P by birds was observed in phytase supplemental groups T2 and T3 (p<0.05. Dry weight of tibia (2.58-2.78 g/kg live weight and ash content (39.7- 41.8% was comparable among treatments. A similar trend was observed for bone Ca, P, and Mn content. Conclusion: The study indicated that 500 FTU/kg phytase can be effectively supplemented in a broiler diet with low phosphorus (0.37% in starter and 0.32% NPP in finisher diet and low calcium (0.8% in diet for better growth performance and with successful replacement of dietary P by 0.08 % and reduced P excretion into the environment in broiler chicken.

  13. Effect of macronutrient ratio of the pre-starter diet on broiler performance and intermediary metabolism

    OpenAIRE

    SWENNEN, Quirine; Everaert, N.; Debonne, M.; Verbaeys, I.; Careghi, C.; Tona, K.; Janssens, G. P. J.; Decuypere, E.; Bruggeman, V.; Buyse, J.

    2010-01-01

    P>The aim of this study was to investigate the influence of isoenergetic substitution between the three energy delivering macronutrients in pre-starter diets on performance and intermediary nutrient metabolism in broiler chickens. From hatch until 5 days of age, 600 chicks, collected during peak of hatch, were fed one of the three experimental pre-starter diets with isoenergetic (13 MJ metabolisable energy/kg) substitutions between fat (43 vs. 108 g/kg), protein (126 vs. 240 g/kg) and carbohy...

  14. Evaluation of The Economic Efficacy of Some Antimycotoxicosis Compounds on Production and Humoral Immunity in Broiler Chickens.

    Directory of Open Access Journals (Sweden)

    Amal H.T. Abdelnaser

    2017-10-01

    Full Text Available Mycotoxicosis is an important problem in poultry industry causing global economic losses constituting a great threat. The present study was designed to investigate the ameliorative effects of four different antimycotoxicosis compounds (AMCs against production, economic performance and humoral immunity induced by intoxication of dietary aflatoxin (AF and/or ochratoxin (OT in broiler chicks. Experiment (I, AF (23 ppb and OT (17 ppb were fed alone or mixed and in association with antimycotoxin feed additives, product A or B (1 and 0.5 g/kg feed, respectively. In experiment (II, the intoxicated chicks treated with antimycotoxin drugs, product C or D in drinking water (1ml/liter/3 successive days and 0.5 ml/liter/one day, respectively. Results revealed that chicks intoxicated with AF and/or OT showed significant (P0.05 change in FCR and ND antibody titers.

  15. Economic Impacts of White Chick Syndrome in Ontario, Canada.

    Science.gov (United States)

    Long, Kathleen E; Hastie, Gordon M; Ojkić, Davor; Brash, Marina L

    2017-09-01

    A cluster of 12 cases of White Chick Syndrome (WCS) in broiler breeder flocks producing affected progeny occurred from June to November 2015 in two broiler chicken hatcheries owned by a single company in Ontario, Canada. Cases were identified by the presence of typical chicks in the hatchery characterized by pale to white down, enlarged abdomens, and occasionally brown wiry fluff on the dorsum of the neck that were generally weak. Affected broiler breeder flocks experienced egg production drops of 0% to 15% and hatchability drops of 1.8% to 49.1%. Some flocks experienced increased feed clean-up duration and/or reduced hatching egg weight. The financial impacts of WCS to affected hatching egg producers averaged $5,912 CAD (US$4,417) per 10 000 hens and were as great as $16,788 CAD (US$12,544) per 10 000 hens. The financial impacts of WCS to the affected hatcheries averaged $1,723 CAD (US$1,287) per 10 000 broiler breeder hens and were as great as $4,096 (US$3,060) per 10 000 hens.

  16. Performance and carcass quality of broiler chickens fed diet containing pineapple waste meal fermented by “ragi tape”

    Science.gov (United States)

    Mandey, J. S.; Tulung, B.; Leke, J. R.; Sondakh, B. F. J.

    2018-01-01

    The study was conducted to determine the effect of pineapple waste meal fermented by “ragi tape” (FPW) in diets on the performance and carcass quality of broilers. The “ragi tape” was a traditional commercial product of yeast. Five dietary treatments containing 0, 5, 10, 15 and 20% levels of FPW with four replicates were fed to 250 broiler chickens for 42 days in a completely randomized design. Feed and water were provided ad libitum. The variables were performance parameters and carcass quality. Results showed that the performance in finisher, carcass percentage and abdominal fat percentage were significantly affected by dietary treatments. Carcass percentage and abdominal fat percentage were significantly decrease in the proportion of 20% of FPW. However, the carcass percentage in treatments R0 - R4 were still in a good category. The higher the levels of FPW the lower the abdominal fat percentage signed that FPW treatments up to 20% resulted good category of broiler carcass. Income over feed cost and broiler cost analysis in 20% FPW treatment obtained the highest income and the lowest was 0% FPW treatment. It can be concluded that FPW can be fed to broiler chickens at up to 20% level.

  17. Live performance and processing yields of broilers fed diets with tiamulin and salinomycin combinations

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    SL Vieira

    2010-03-01

    Full Text Available A study was conducted with the objective of evaluating the combined application of tiamulin (TIA and salinomycin (SAL in broiler diets fed from 1 to 42 d of age. One thousand and two hundred Cobb x Cobb 500 male broilers were housed in 48 floor pens and fed corn-soybean meal diets containing 66 ppm of SAL combined or not with TIA at 30 or 20 and 20 or 15 ppm, respectively, in the starter (1-21 d and grower feeds (22-42 d; however, TIA was withdrawn from the feeds 7 days before slaughter. The experimental design was completely randomized with 3 treatments and 16 replicates of 25 birds each. Broilers were weekly evaluated for live performance whereas carcass yield, abdominal fat and commercial cuts were assessed at 42 d using 6 birds randomly taken from each pen. Results obtained at the end of the study demonstrated that body weight gain was not affected (P > 0.05 by the treatments, whereas feed intake was reduced (P 0.05 by the treatments. Live performance and post-slaughter yields data obtained in this study did not indicate that combinations of TIA with SAL could be detrimental. In fact, an improvement in feed conversion was observed at the lowest dose of TIA.

  18. Infrared thermography applied to the evaluation of metabolic heat loss of chicks fed with different energy densities

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    VMOS Ferreira

    2011-06-01

    Full Text Available Brazil must comply with international quality standards and animal welfare requirements in order to maintain its position as world's largest exporter of poultry meat. With the scenario of global climate change there is the forecast of occurrence of extreme events with characteristics of both excess cold and heat for several regions of the country. This study aimed to evaluate the effectiveness of using images of infrared thermography to evaluate the loss of sensible heat in young broilers fed different dietary energy levels. Twenty birds were reared in a house with appropriate brooding using infrared lamps. Birds were distributed in a completely randomized experimental into two treatments: T1 (control diet with 2950 kcal ME/kg-1, and T2 (high-energy diet with 3950 kcal ME/kg-1. Infrared thermographic images of the birds were recorded for four consecutive days. One bird was randomly chosen per treatment, and had special images taken and analyzed. Average surface temperature of the body area was calculated using the surface temperature recorded at 100 spots (50 at the front and 50 at the lateral side of the bird's body. Mean surface temperature of the flock was calculated recording 100 spots on the group of birds. Total radiant heat loss was calculated based on the average data of surface temperature. The results indicated that the young broilers fed the high-energy diet presented a metabolic energy loss equivalent to 0.64 kcal h-1, while the birds fed with the control diet lost 2.18 kcal h-1. This finding confirms that oil supplementation to the diet reduces bird heat loss. The infrared camera was able to record young broilers' surface temperature variation when birds were fed diets with different energy contents.

  19. Growth, immune, antioxidant, and bone responses of heat stress-exposed broilers fed diets supplemented with tomato pomace

    Science.gov (United States)

    Hosseini-Vashan, S. J.; Golian, A.; Yaghobfar, A.

    2016-08-01

    A study was conducted to investigate the effects of supplementation of dried tomato pomace (DTP) on growth performance, relative weights of viscera, serum biological parameters, antioxidant status, immune response, and bone composition of broilers exposed to a high ambient temperature. A total of 352 one-day-old male broiler chickens were randomly divided into four groups consisting of four replicates with 22 birds each. One group was reared under the thermoneutral zone and fed a corn-soybean meal basal diet. The other three groups were subjected to a cyclic heat stress from 29 to 42 days of age (34 ± 1 °C, 55 % RH, 5 h/day). These birds were fed corn-soybean meal basal diet or the same diet supplemented with 3 % DTP (420 mg lycopene/kg diet) or 5 % (708 mg lycopene/kg diet) of DTP. Blood samples were collected on days 28 and 42, and the birds were slaughtered at the same times. Supplementation of 5 % of DTP increased body weight and production index and decreased feed conversion ratio during 1-28 days of age. On day 28, the broilers supplemented with 5 % DTP had lower serum triglycerides and higher high-density lipoprotein (HDL) cholesterol concentration than those on the other dietary treatments. The activities of glutathione peroxidase (GPx) and superoxide dismutase (SOD) were higher and the concentration of malondialdehyde (MDA) was lower in the broilers fed 5 % TP than those of the broilers fed other diets at 28 days of age. The effects of heat stress (HS) were impaired body weight, enhanced serum activities of alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, lipase, and MDA concentration while reducing the activities of GPx and SOD. Dried tomato pomace supplementation did not influence growth performance under HS but ameliorated the negative effects of HS on the serum enzyme activities, GPx activity, and lipid peroxidation. Heat stress did not change the relative weights of the lymphoid organs but reduced the total and IgG titers

  20. Influence of Feed Supplementation with Cannabis Sativa on Quality of Broilers Carcass

    Directory of Open Access Journals (Sweden)

    Rifat Ullah Khan1*, F. R. Durrani1, Naila Chand1 and Haseeb Anwar

    2010-01-01

    Full Text Available A project was planned to study the effect of feeding powdered seeds of Cannabis sativa on the carcass quality of broiler chicks. A total of 160 day-old broiler chicks of equal weight were randomly divided into four equal groups A, B, C and D. Each group was further divided into four replicates with 10 chicks in each replicate. Dried crushed Cannabis sativa seeds were added to the feed of groups B, C and D at the rate of 5, 10 and 20% of offered feed respectively, while group A served as a control. The studied parameters were body weight, feed intake, feed conversion ratio (FCR, dressing percentage, mortality and economics. After an experimental period of 42 days, the data were analyzed statistically. It was revealed from the results that body weight gain was significantly higher (P<0.05, while feed intake was significantly lower (P<0.05, in group D compared to the control. FCR was significantly better in birds of group D compared to controls. Differences in dressing percentage and mortality were non significant between the treated and control groups. Return per chick (in rupees was significantly higher in group D compared to groups A and B (P<0.05. It was concluded from these results that seeds of Cannabis sativa have remarkable impact on growth of broiler chicks and can help in alleviating feed expenditure incurred on raising broiler chicks.

  1. Peripheral Insulin Doesn’t Alter Appetite of Broiler Chicks

    Directory of Open Access Journals (Sweden)

    Lei Liu

    2016-09-01

    Full Text Available An experiment was conducted to investigate the effect of peripheral insulin treatment on appetite in chicks. Six-d-age chicks with ad libitum feeding or fasting for 3 h before injection received a subcutaneous injection of 0, 1, 3, 5, 10, or 20 IU of insulin or vehicle (saline. The results showed peripheral insulin treatment (1 to 20 IU did not alter significantly the feed intake in chicks under either ad libitum feeding or fasting conditions within 4 h (p>0.05. Compared with the control, plasma glucose concentration was significantly decreased after insulin treatment of 3, 5, 10, and 20 IU for 4 h in chicks with ad libitum feeding (p0.05. All results suggest peripheral administration of insulin has no effect on appetite in chicks.

  2. Nutritional Evaluation of Distillery Sludge and Its Effect as a Substitute of Canola Meal on Performance of Broiler Chickens

    Science.gov (United States)

    Sharif, M.; Shahzad, M. A.; Rehman, S.; Khan, S.; Ali, R.; Khan, M. L.; Khan, K.

    2012-01-01

    The study was conducted to investigate the chemical composition of distillery yeast sludge and its inclusion in broiler diets to replace canola meal. Raw distillery yeast sludge was washed with water using water and sludge in the ratio 6:1, respectively. Proximate analysis of raw distillery yeast sludge and washed distillery sludge was carried out for crude protein (CP), true protein (TP), ether extract (EE), ash, acid insoluble ash and nitrogen free extract (NFE) determination. Mineral contents and amino acid profile of raw distillery yeast sludge and washed distillery sludge were also determined. After chemical evaluation, four iso-caloric and iso-nitrogenous broiler starter and finisher diets were prepared in mash form using 0 (control), 4, 8 and 12% levels of washed distillery sludge replacing canola meal. One hundred and twenty day-old broiler chicks were randomly distributed into 12 experimental units in such a way that each diet was offered to three experimental units, each comprising of 10 chicks. It was observed that washing affected the nutrients either by decreasing or increasing their concentration. It decreased the total mineral contents whereas CP, TP, EE and NFE contents increased. Washing also increased amino acid profile. Average feed intake and weight gain were higher in birds fed diet containing 8% washed distillery sludge and lower in birds fed diet containing 0% washed distillery sludge. Feed cost per kg live weight gain decreased significantly as the level of washed distillery sludge was increased in the diet. Average heart, liver and pancreas weights decreased with increased level of washed distillery sludge in the diet. The study revealed that after washing, distillery yeast sludge can be used successfully in broiler diets up to the level of 8% without any adverse effect on broiler’s performance. PMID:25049579

  3. Nutritional Evaluation of Distillery Sludge and Its Effect as a Substitute of Canola Meal on Performance of Broiler Chickens

    Directory of Open Access Journals (Sweden)

    M. Sharif

    2012-03-01

    Full Text Available The study was conducted to investigate the chemical composition of distillery yeast sludge and its inclusion in broiler diets to replace canola meal. Raw distillery yeast sludge was washed with water using water and sludge in the ratio 6:1, respectively. Proximate analysis of raw distillery yeast sludge and washed distillery sludge was carried out for crude protein (CP, true protein (TP, ether extract (EE, ash, acid insoluble ash and nitrogen free extract (NFE determination. Mineral contents and amino acid profile of raw distillery yeast sludge and washed distillery sludge were also determined. After chemical evaluation, four iso-caloric and iso-nitrogenous broiler starter and finisher diets were prepared in mash form using 0 (control, 4, 8 and 12% levels of washed distillery sludge replacing canola meal. One hundred and twenty day-old broiler chicks were randomly distributed into 12 experimental units in such a way that each diet was offered to three experimental units, each comprising of 10 chicks. It was observed that washing affected the nutrients either by decreasing or increasing their concentration. It decreased the total mineral contents whereas CP, TP, EE and NFE contents increased. Washing also increased amino acid profile. Average feed intake and weight gain were higher in birds fed diet containing 8% washed distillery sludge and lower in birds fed diet containing 0% washed distillery sludge. Feed cost per kg live weight gain decreased significantly as the level of washed distillery sludge was increased in the diet. Average heart, liver and pancreas weights decreased with increased level of washed distillery sludge in the diet. The study revealed that after washing, distillery yeast sludge can be used successfully in broiler diets up to the level of 8% without any adverse effect on broiler’s performance.

  4. Effect of citric acid and microbial phytase on serum enzyme activities ...

    African Journals Online (AJOL)

    Effect of citric acid and microbial phytase on serum enzyme activities and plasma minerals retention in broiler chicks. ... African Journal of Biotechnology ... An experiment was conducted to study the effect of microbial phytase supplementation and citric acid in broiler chicks fed corn-soybean meal base diets on enzyme ...

  5. Effects of lactic acid bacteria and smectite after aflatoxin B1 challenge on the growth performance, nutrient digestibility and blood parameters of broilers.

    Science.gov (United States)

    Liu, N; Ding, K; Wang, J; Deng, Q; Gu, K; Wang, J

    2018-04-11

    This study aimed to investigate the effect of lactic acid bacteria (LAB) and smectite on the growth performance, nutrient digestibility and blood parameters of broilers that were fed diets contaminated with aflatoxin B 1 (AFB 1 ). A total of 480 newly hatched male Arbor Acres broilers were randomly allocated into four groups with six replicates of 20 chicks each. The broilers were fed diets with the AFB 1 (40 μg/kg) challenge or without (control) it and supplemented with smectite (3.0 g/kg) or LAB (4.0 × 10 10  CFU/kg) based on the AFB 1 diet. The trial lasted for 42 days. The results showed that during days 1-42 of AFB 1 challenge, the feed intake (FI) and body weight gain (BWG) were depressed (p smectite increased (p smectite. LAB and smectite also increased (p smectite affected (p smectite have similar effects on the growth and health of the broilers, suggesting that LAB could be an alternative against AFB 1 in commercial animal feeds. © 2018 Blackwell Verlag GmbH.

  6. Efficacy of New 6-Phytase from Buttiauxella spp. on Growth Performance and Nutrient Retention in Broiler Chickens Fed Corn Soybean Meal-based Diets

    Science.gov (United States)

    Kiarie, E.; Woyengo, T.; Nyachoti, C. M.

    2015-01-01

    A total of 420 day-old male Ross chicks were weighed at d 1 of life and assigned to test diets to assess the efficacy of a new Buttiauxella spp. phytase expressed in Trichoderma reesei. Diets were: positive control (PC) adequate in nutrients and negative control (NC) diet (40% and 17% less available phosphorous (P) and calcium (Ca), respectively) supplemented with 6 levels of phytase 0, 250, 500, 750, 1,000, and 2,000 phytase units (FTU)/kg of diet. All diets had titanium dioxide as digestibility marker and each diet was allocated to ten cages (6 birds/cage). Diets were fed for 3 wk to measure growth performance, apparent retention (AR) on d 17 to 21 and bone ash and ileal digestibility (AID) on d 22. Growth performance and nutrient utilization was lower (pPhytase response in NC birds was linear (pphytase at ≥750 FTU resulted in AID of total AA commensurate to that of PC fed birds and at ≥1,000 FTU improved (pphytase and PC diet. In conclusion, the result from this study showed that in addition to increased P and Ca utilization, the new Buttiauxella phytase enhanced growth performance and utilization of other nutrients in broiler chickens in a dose-dependent manner. PMID:26323404

  7. Dietary l-threonine supplementation attenuates lipopolysaccharide-induced inflammatory responses and intestinal barrier damage of broiler chickens at an early age.

    Science.gov (United States)

    Chen, Yueping; Zhang, Hao; Cheng, Yefei; Li, Yue; Wen, Chao; Zhou, Yanmin

    2018-06-01

    This study was conducted to investigate the protective effects of l-threonine (l-Thr) supplementation on growth performance, inflammatory responses and intestinal barrier function of young broilers challenged with lipopolysaccharide (LPS). A total of 144 1-d-old male chicks were allocated to one of three treatments: non-challenged broilers fed a basal diet (control group), LPS-challenged broilers fed a basal diet without l-Thr supplementation and LPS-challenged broilers fed a basal diet supplemented with 3·0 g/kg l-Thr. LPS challenge was performed intraperitoneally at 17, 19 and 21 d of age, whereas the control group received physiological saline injection. Compared with the control group, LPS challenge impaired growth performance of broilers, and l-Thr administration reversed LPS-induced increase in feed/gain ratio. LPS challenge elevated blood cell counts related to inflammation, and pro-inflammatory cytokine concentrations in serum (IL-1β and TNF-α), spleen (IL-1β and TNF-α) and intestinal mucosa (jejunal interferon-γ (IFN-γ) and ileal IL-1β). The concentrations of intestinal cytokines in LPS-challenged broilers were reduced by l-Thr supplementation. LPS administration increased circulating d-lactic acid concentration, whereas it reduced villus height, the ratio between villus height and crypt depth and goblet density in both jejunum and ileum. LPS-induced decreases in jejunal villus height, intestinal villus height:crypt depth ratio and ileal goblet cell density were reversed with l-Thr supplementation. Similarly, LPS-induced alterations in the intestinal mRNA abundances of genes related to intestinal inflammation and barrier function (jejunal toll-like receptor 4, IFN- γ and claudin-3, and ileal IL-1 β and zonula occludens-1) were normalised with l-Thr administration. It can be concluded that l-Thr supplementation could attenuate LPS-induced inflammatory responses and intestinal barrier damage of young broilers.

  8. Anticoccidial effect of Piper sarmentosum extracts in experimental coccidiosis in broiler chickens.

    Science.gov (United States)

    Wang, Dingfa; Zhou, Luli; Li, Wei; Zhou, Hanlin; Hou, Guanyu

    2016-06-01

    To study the anticoccidial effect of Piper sarmentosum extracts (PSE) in experimental broiler coccidiosis, 270 one-day-old Wenchang broiler chickens were randomly assigned to six groups, each with three replicates (n = 15). The six groups were blank control group (BC), negative control group (NC), positive control group (PC), and another three PSE addition groups. Chickens in three control groups were fed a basal diet without PSE supplementation. Chickens in the three PSE addition groups were fed a basal diet supplemented with PSE at 100 (T100), 200 (T200), and 300 (T300) mg/kg of feed, respectively. At 15 days of age, chickens in group NC, PC, and three PSE addition groups were challenged with an oral dose of 1 × 10(5) Eimeria tenella oocysts each chick. Chickens in group PC were fed with diclazuril solution in water for 5 days after 48 h with oocysts inoculation. The results showed that PSE and diclazuril improved growth performance and significantly (P < 0.05) decreased oocysts per gram in inoculated broiler chickens. PSE and diclazuril significantly (P < 0.05) decreased nitric oxide at 6 and 9 days post-inoculation relative to the NC group, respectively. At 6 and 9 days post-inoculation, PSE supplementation at 200 mg/kg in the diet increased concentration of interleukin 2 (IL-2) and interferon gamma (IFN-γ) (P < 0.05). PSE supplementation at 200 mg/kg in the diet significantly (P < 0.05) increased mRNA expressions of IFN-γ and IL-2 in the cecum of chickens at 9 days post-inoculation relative to the BC and NC group. The current results showed the anticoccidial properties, and beneficial effect on intestinal mucosa damage of PSE in broiler chickens that had been challenged by coccidiosis.

  9. Influence of combinations of fenugreek, garlic, and black pepper powder on production traits of the broilers

    Science.gov (United States)

    Kirubakaran, A.; Moorthy, M.; Chitra, R.; Prabakar, G.

    2016-01-01

    Aim: To study the effects of combinations of fenugreek (Trigonella foenum-graecum L.), garlic (Allium sativum), and black pepper (Piper nigrum) powder supplementation on production traits of broiler chickens. Materials and Methods: A total of 288 commercial broiler chicks were randomly assigned to 1-9 groups with 4 replicates each. An experiment was conducted in broilers with different feed formulations; control feed, with no added fenugreek, garlic, and black pepper powder; and 8 treatment groups receiving feed supplemented with different combinations of fenugreek, garlic, and black pepper powder. The individual broilers’ body weight and feed consumption were recorded and calculate the body weight gain and feed conversion ratio (FCR). Results: Broiler’s weight gain and FCR were significantly higher in groups receiving feed supplemented with garlic and black pepper powder combinations (ppepper powder combinations (ppepper powder supplemented broiler feed fed groups showed higher production performance. The 5 g/kg garlic powder+1 g/kg black pepper powder and 10 g/kg garlic powder+2 g/kg black pepper powder significantly improved the weight gain and FCR. PMID:27284222

  10. Bioaccumulation of Heavy Metals in Broilers Fed “Trinidad de Guedes” Phosphorite

    Directory of Open Access Journals (Sweden)

    Valera M

    2017-06-01

    Full Text Available In order to use the “Trinidad de Guedes" phosphorite (TGP as a supplement of calcium and phosphorus in animals, its concentrations of heavy metals were determined and their deposition was analyzed in broilers. Toxic elements were determined using atomic absorption spectrophotometry. The mean levels of heavy metals found in TGP were 82.5 mg/kg lead (Pb, 7.2 mg/kg cadmium (Cd, 1056.9 mg/kg manganese (Mn, 1.3 mg/kg mercury (Hg, 10.4 mg/kg  arsenic (As, 210.6 mg/kg zinc (Zn, and 121.3 mg/kg copper (Cu. All concentrations of heavy metals were below harmful levels, with the exception of Pb, which was above its maximum tolerable value. The levels of heavy metals accumulated in bones and feathers of broilers fed TGP were determined in a feeding trial. An amount of 300 day-old broilers from commercial hybrid crossing (HE21 were allocated into three treatments in a completely randomized design, with four replicates of 25 broilers each. The three treatments were a control containing dicalcium phosphate as calcium and phosphorus source, and two experimental treatments with TGP replacing dicalcium phosphate at levels of 50 and 100%. There were no differences between treatments for levels of toxic elements accumulated in feathers, femurs and tarsus of broilers. The amounts of heavy metals accumulated in the analyzed organs showed that TGP can replace dicalcium phosphate (DCP, the traditional source of calcium and phosphate without causing harm to animal health nor bioaccumulating heavy metals.

  11. Effects of aqua agar as water replacement for posthatch chicks during transportation on residual yolk-sac and growth performance of young broiler chickens

    Directory of Open Access Journals (Sweden)

    Tossaporn Incharoen

    2015-12-01

    Full Text Available Water is one of the most essential nutrients for the maintenance of chicks' function, and delayed access to feed and water post hatch has been reported to dehydrate chicks. Aqua agar (AA was formulated to contain more than 95% water and an experiment was conducted to evaluate the effects of AA as water replacement for posthatch chicks during transportation. During the simulated transport, chicks were held for 24 h with (AA group or without (NO-AA group aqua agar in chick boxes. During the holding period, chicks in the AA group lost less body weight, compared with the NO-AA group. However, the weight of residual yolk tended to be lower in the AA-treated birds. There were no significant differences in the weight of gizzard, proventriculus, and liver, nor in the weights and lengths of the duodenum, jejunum and ileum. A higher body weight was also observed in the AA group at 7 days of age. At 21 days of age, weight gain and feed intake were higher (P < 0.05 in the AA group, when compared to that of the NO-AA group. No significant differences were observed in the feed conversion rate (FCR between the two groups. In conclusion, the data suggests that the use of AA as a water replacement could reduce the negative impact of water deficiency in posthatch period during transportation, resulting in greatly improved growth performance of young broilers at 21 days of age.

  12. Digestible Threonine Levels in the Starter Diet of Broilers Derived from Breeders of Different Ages

    Directory of Open Access Journals (Sweden)

    CBGS Tanure

    2015-12-01

    Full Text Available ABSTRACT The aim of this study was to evaluate the effect of digestible threonine supplementation in the starter diet on the performance, intestinal parameters, and nutrient metabolism of broilers derived from breeders of different ages. In total, 480 one-day-old Cobb chicks, derived from 38-or 49-week-oldbreeders, were housed in experimental battery cages until 21 days of age and fed four different threonine levels (800, 900, 1,000, or 1,100 mg/kg in the starter feed. A completely randomized experimental design in a 2x4 factorial arrangement (breeder age x threonine levels was applied, totaling eight treatments with five replicates of 12 birds each. Broilers from older breeders fed 800 mg digestible threonine/kg of diet presented higher weight gain, with a positive linear effect. There was also an interaction between breeder age and threonine levels for the weight gain of 21-d-old broilers supplemented at maximum level of 1,003 mg Thr/kg diet during the starter phase. There was no effect of breeder age or threonine levels on nutrient metabolism during the period of 17-21 days. There was no influence of breeder age or threonine levels in the starter diet on intestinal morphometric measurements, absorption area, or percentage of goblet cells.

  13. STUDY ON EFFICACY OF DIATOMACEOUS EARTH TO AMELIORATE AFLATOXIN - INDUCED PATHO-MORPHOLOGICAL CHANGES IN LYMPHOID ORGANS OF BROILER CHICKEN

    Directory of Open Access Journals (Sweden)

    A.W. Lakkawar

    2017-12-01

    Full Text Available The efficacy of Diatomaceous earth (DAE in reducing the detrimental effects of aflatoxin (AF in broiler diet was evaluated. DAE was supplemented 2000 mg/kg of feed along with 0.5 and 1 ppm of AF in feed. A total of 240 healthy day old broiler chicks were divided into 6 groups comprising of control and treatment groups. Feeding of AF resulted in reduction in size of the thymus, spleen and bursa of Fabricius. In addition, petechial haemorrhages were observed on the surface of the thymus. Histopathology revealed varying degree of lymphocytolysis and depletion of lymphoid cells in thymus, spleen and bursa of Fabricius. In addition, the ceacal tonsils also revealed a mild to moderate degree of lymphoid depletion. The supplementation of DAE to aflatoxin-mixed feed revealed significant improvement characterised by decreased severity of lesions in lymphoid organs. The macroscopic and microscopic changes in the birds fed DAE in combination with AF included those that were observed in AF- alone fed birds, but of reduced magnitude and severity. The study concluded that 2% DAE in feed can be effectively used to reduce the the histotoxic effects of aflatoxin on lymphoid organs in broiler chicken.

  14. Effects of Replacing Soybean Meal with Fermented Rapeseed Meal on Performance, Serum Biochemical Variables and Intestinal Morphology of Broilers

    Directory of Open Access Journals (Sweden)

    F. Z. Xu

    2012-12-01

    Full Text Available This trial was performed to study the effects of replacing soybean meal (SBM with fermented rapeseed meal (RSM on growth performance, serum biochemistry variable and intestinal morphology of broilers. A total of 640 d-old Arbor Acres broiler chicks were randomly allocated to 4 dietary treatments, 4 pens per treatment and 40 birds per pen for a 6-wk feeding trial. In the four treatment groups, fermented RSM replaced soybean meal at 0, 5, 10, and 15%, respectively. On 21 d and 42 d, two birds from each pen were randomly selected and slaughtered. Blood samples and sections of duodenum, jejunum, and ileum were collected for measurement of serum biochemical variables and intestinal morphology, respectively. Results showed that body weight gain (BWG and feed conversion (FC were significantly (p<0.01 poorer for birds fed the 15% fermented RSM diet than those fed with 0, 5 and 10% fermented RSM diets during all periods. Compared with 0 and 5% fermented RSM groups, IgG content in the serum of birds in 10 and 15% fermented RSM groups was improved (p<0.01 urea nitrogen content of serum was reduced (p<0.01 during both growing and finishing periods. However, IgM, phosphorus and calcium levels increased (p<0.05 only during the growing period. Increased (p<0.05 villus height was observed in the duodenum and jejunum of broilers fed the diet with 10% fermented RSM. In addition, villus height to crypt depth ratio in the jejunum was significantly higher (p<0.01 for birds fed the diet with 10% fermented RSM than for those fed diets with 0, 5 and 15% fermented RSM. The present results suggest that RSM fermented with Lactobacillus fermentum and Bacillus subtilis is a promising alternative protein source and that it could be safely used replace up to 10% SBM in broiler diets.

  15. Effect of Zinc on Humoral and Cell-Mediated Immunity of Broilers Vaccinated Against Coccidiosis

    Directory of Open Access Journals (Sweden)

    Milad Moazeni

    2013-09-01

    Full Text Available Background: The aim of the present study was the comparison of humoral and cell-mediated immunity in ‎broilers fed with different levels of zinc during a coccidiosis challenge.‎Methods: One hundred and forty-‎four one-day-old broiler chicks were used with three ‎dietary zinc ‎(40, 120 and 200 mg/kg. At 14 d of age, all birds were inoculated orally with 5×103 sporulated oocysts of E. Tenella. ‎At ‎2, 22, 32, 42 ‎days of age, the blood serums were tested for ‎antibody titer against‎ Newcas­tle disease vaccine, using ‎the standard HI test. On day 42 the sum of nitrite ‎and nitrate based on the reduction of nitrate ‎to nitrite by cadmium ‎and white blood cell count (WBC using a hemocytometer were measured.Results: At 42 d, levels of ‎120 and 200 mg significantly (P< 0.05 increased the antibody titer in compare with the control. The peak response of CBH was observed at the level of 200 mg Zn/kg diet. Also both level of 120 and 200 mg Zn/kg diet increased WBC count and sum of nitrite and nitrate‎ in serum compared with the control.Conclusion: The levels of 120 and 200 mg Zn/kg diet could be considered as a non-pharmacologic booster of immunity in broilers chicks infected with E. Tenella.

  16. Effects of dietary fructooligosaccharide on digestive enzyme activities, intestinal microflora and morphology of male broilers.

    Science.gov (United States)

    Xu, Z R; Hu, C H; Xia, M S; Zhan, X A; Wang, M Q

    2003-06-01

    Two hundred forty male Avian Farms broiler chicks, 1 d of age, were randomly allocated to four treatments, each of which had five pens of 12 chicks per pen. The chicks were used to investigate the effects of fructooligosaccharide (FOS) on digestive enzyme activities and intestinal microflora and morphology. The chicks received the same basal diet based on corn-soybean meal, and FOS was added to the basal diet at 0, 2.0, 4.0, and 8.0 g/kg diet at the expense of corn. Addition of 4.0 g/kg FOS to the basal diet significantly increased average daily gain of broilers. The feed-to-gain ratios were significantly decreased for the birds fed diets with 2.0 and 4.0 g/kg FOS versus the control. Addition of 4.0 g/kg FOS enhanced the growth of Bifidobacterium and Lactobacillus, but inhibited Escherichia coli in the small intestinal and cecal digesta. Supplementation of 2.0 or 4.0 g/kg FOS to chicks significantly improved the activities of amylase compared to the control (12.80 or 14.75 vs. 8.42 Somogyi units). A significant increase in the activities of total protease was observed in 4.0 g/kg FOS-treated birds versus controls (83.91 vs. 65.97 units). Morphology data for the duodenum, jejunum, and ileum showed no significant differences for villus height, crypt depth, or microvillus height at the duodenum. By contrast, addition of 4.0 g/kg FOS significantly increased ileal villus height, jejunal and ileal microvillus height, and villus-height-to-crypt-depth ratios at the jejunum and ileum and decreased crypt depth at the jejunum and ileum. However, addition of 8.0 g/kg FOS had no significant effect on growth performance, digestive enzyme activities, intestinal microflora, or morphology.

  17. Effects of Heat-oxidized Soy Protein Isolate on Growth Performance and Digestive Function of Broiler Chickens at Early Age

    Directory of Open Access Journals (Sweden)

    X. Chen

    2015-04-01

    Full Text Available This study was conducted to investigate effects of heat-oxidized soy protein isolate (HSPI on growth performance, serum biochemical indices, apparent nutrient digestibility and digestive function of broiler chickens. A total of 320 1-day-old Arbor Acres chicks were randomly divided into 4 groups with 8 replicates of 10 birds, receiving diets containing soy protein isolate (SPI, control group or the same amount of SPI heated in an oven at 100°C for 1, 4, or 8 h, for 21 days, respectively. The results indicated that compared with the control group, body weight gain and feed intake of birds fed diet containing SPI heated for 8 h were significantly lower (p<0.05. Serum urea nitrogen concentration was higher in the broilers fed diet containing SPI heated for 4 or 8 h at d 21 (p<0.05. In contrast, serum glucose content was decreased by HSPI substitution at d 21 (p<0.05. The relative pancreas weight in HSPI groups was higher than that in the control group at d 21 (p<0.05. Meanwhile, the opposite effect was observed for relative weight of anterior intestine and ileum in broilers fed a diet containing SPI heated for 8 h (p<0.05. Birds fed diets containing SPI heated for 4 or 8 h had a decreased lipase activity in anterior intestinal content at d 14 and 21 (p<0.05, respectively. In addition, the same effect was also noted in broilers given diets containing SPI heated for 1 h at d 21 (p<0.05. Similarly, amylase, protease and trypsin activity in anterior intestinal content were lower in broilers fed diets containing SPI heated for 8 h at d 21 (p<0.05. The apparent digestibility of dry matter (DM from d 8 to 10 and DM, crude protein (CP, and ether extract from d 15 to 17 were lower in broilers fed diets containing SPI heated for 8 h (p<0.05. Besides, birds given diets containing SPI heated for 4 h also exhibited lower CP apparent digestibility from d 15 to 17 (p<0.05. It was concluded that HSPI inclusion can exert a negative influence on the growth

  18. Effect of effective microorganisms on broiler chicken performance ...

    African Journals Online (AJOL)

    A study was conducted between January and March 2001 to assess the effects of Effective Microorganisms (EM) as feed additive in broiler chicken production on growth performance. The experiment involved 210 day-old broiler chicks which were randomly allocated to 14 pens of 15 birds each. There were seven ...

  19. Two necrotic enteritis predisposing factors, dietary fishmeal and Eimeria infection, induce large changes in the caecal microbiota of broiler chickens.

    Science.gov (United States)

    Wu, Shu-Biao; Stanley, Dragana; Rodgers, Nicholas; Swick, Robert A; Moore, Robert J

    2014-03-14

    It is widely established that a high-protein fishmeal supplemented starter diet and Eimeria infection can predispose birds to the development of clinical necrotic enteritis symptoms following Clostridium perfringens infection. However, it has not been clearly established what changes these treatments cause to predispose birds to succumb to necrotic enteritis. We analysed caecal microbiota of 4 groups of broilers (n=12) using deep pyrosequencing of 16S rDNA amplicons: (1) control chicks fed a control diet, (2) Eimeria infected chicks fed control diet, (3) chicks fed fishmeal supplemented diet and lastly (4) both fishmeal fed and Eimeria infected chicks. We found that the high-protein fishmeal diet had a strong effect on the intestinal microbiota similar to the previously reported effect of C. perfringens infection. We noted major changes in the prevalence of various lactobacilli while the total culturable Lactobacillus counts remained stable. The Ruminococcaceae, Lachnospiraceae, unknown Clostridiales and Lactobacillaceae families were most affected by fishmeal with increases in a number of operational taxonomic units (OTUs) that had previously been linked to Crohn's disease and reductions in OTUs known to be butyrate producers. Eimeria induced very different changes in microbiota; Ruminococcaceae groups were reduced in number and three unknown Clostridium species were increased in abundance. Additionally, Eimeria did not significantly influence changes in pH, formic, propionic or isobutyric acid while fishmeal induced dramatic changes in all these measures. Both fishmeal feeding and Eimeria infection induced significant changes in the gut microbiota; these changes may play an important role in predisposing birds to necrotic enteritis. Copyright © 2014 Elsevier B.V. All rights reserved.

  20. Effects of peach palm oil on performance, serum lipoproteins and haemostasis in broilers.

    Science.gov (United States)

    Baldizán, G; Oviedo, M; Michelangeli, C; Vargas, R E

    2010-12-01

    1. An experiment was conducted to study the comparative effects of peach palm oil (PPO, Bactris gasipaes H.B.K), crude palm oil (CPO, Elaeis guinenesis), maize oil (MO) and beef tallow (BT) on serum total and lipoprotein cholesterol levels and haemostatic factors in broiler chickens. 2. Four experimental diets were formulated to be isocaloric (14·2 MJ AME(N)/kg) and isonitrogenous (230 g CP/kg). PPO was extracted from the whole dry fruit with hexane. Each fat was added to the diet in an amount equivalent to 25% of total dietary calories. Six replicate groups of eight male broiler chicks were assigned randomly to each dietary treatment. Diets were fed on ad libitum basis. The experiment lasted 42 d. 3. At 42 d, birds were fasted overnight and three chickens/dietary treatment were utilised to draw blood for lipoprotein separation. Various haemostatic factors were determined in thrombocyte-poor plasma. Thrombocyte aggregation was assayed in whole blood. 4. No significant differences were detected in body-weight gain or feed efficiency between the chickens fed on the PPO diet and those receiving the CPO, MO or BT diets. Total serum cholesterol (TC), very low density lipoprotein cholesterol and low density lipoprotein cholesterol (LDL(C)) were not significantly affected after consuming the PPO, CPO and MO diets. Serum high density lipoprotein cholesterol (HDL(C)) was reduced only by the MO diet. Birds fed on the PPO diet had a significantly lower [corrected] LDLC/HDLC ratio compared with other dietary treatments 5. Thrombocyte count and thrombin time were not significantly affected by the experimental diets. Dietary oils significantly affected prothrombin time, fibrinogen concentration and thrombocyte aggregation. PPO and MO diets elicited the lowest fibrinogen levels compared to the CPO and BT diets. Thrombocyte aggregation in broilers fed on the PPO diet was similar to that of the CPO, MO and BT diets. 6. The results suggest that PPO might efficiently provide up to

  1. Cupric citrate as growth promoter for broiler chickens in different rearing stages

    Energy Technology Data Exchange (ETDEWEB)

    Brainer, Monica Maria de Almeida [EAFCe, Ceres, GO (Brazil). Dept. de Zootecnia; Menten, Jose Fernando Machado; Vale, Marcos Martinez do; Morais, Sonia Cristina Daroz de [Universidade de Sao Paulo (USP), Piracicaba, SP (Brazil). Escola Superior de Agricultura Luiz de Queiroz (ESALQ). Dept. de Zootecnia]. E-mail: jfmmente@esalq.usp.br

    2003-07-01

    Feeding cupric citrate as alternative to cupric sulfate to broilers has been suggested in the literature. Day-old male broiler chicks (1,200) were used in an experiment to evaluate the efficacy of cupric citrate supplementation (75 mg Cu kg-1) during the 1-21, 22-42 or 1-42 d periods in comparison to an unsupplemented diet and a diet supplemented with cupric sulfate (200 mg Cu kg-1, 1-42 d). A randomized block design was used, with five treatments, six replicates and 40 birds per pen. The diets, based on corn and soybean meal, and water were offered ad libitum during the 42-day experimental period. Over the entire period, there was no effect of copper supplementation (P > 0.05) on bird live weight, weight gain, feed intake, feed conversion and mortality. Cupric citrate supplementation on the 22-42 d period resulted in worse feed conversion as compared to broilers receiving cupric sulfate (2.014 vs. 1.967, P < 0.05). Copper residues in the litter were reduced when broilers were fed cupric citrate, as compared to cupric sulfate (P < 0.01). The absence of response to copper supplementation can be attributed to the environmental and sanitary rearing conditions.

  2. Cupric citrate as growth promoter for broiler chickens in different rearing stages

    International Nuclear Information System (INIS)

    Brainer, Monica Maria de Almeida; Menten, Jose Fernando Machado; Vale, Marcos Martinez do; Morais, Sonia Cristina Daroz de

    2003-01-01

    Feeding cupric citrate as alternative to cupric sulfate to broilers has been suggested in the literature. Day-old male broiler chicks (1,200) were used in an experiment to evaluate the efficacy of cupric citrate supplementation (75 mg Cu kg-1) during the 1-21, 22-42 or 1-42 d periods in comparison to an unsupplemented diet and a diet supplemented with cupric sulfate (200 mg Cu kg-1, 1-42 d). A randomized block design was used, with five treatments, six replicates and 40 birds per pen. The diets, based on corn and soybean meal, and water were offered ad libitum during the 42-day experimental period. Over the entire period, there was no effect of copper supplementation (P > 0.05) on bird live weight, weight gain, feed intake, feed conversion and mortality. Cupric citrate supplementation on the 22-42 d period resulted in worse feed conversion as compared to broilers receiving cupric sulfate (2.014 vs. 1.967, P < 0.05). Copper residues in the litter were reduced when broilers were fed cupric citrate, as compared to cupric sulfate (P < 0.01). The absence of response to copper supplementation can be attributed to the environmental and sanitary rearing conditions

  3. Validating growth and development of a seabird as an indicator of food availability: captive-reared Caspian Tern chicks fed ad libitum and restricted diets

    Science.gov (United States)

    Lyons, Donald E.; Roby, Daniel D.

    2011-01-01

    For seabirds raising young under conditions of limited food availability, reducing chick provisioning and chick growth rates are the primary means available to avoid abandonment of a breeding effort. For most seabirds, however, baseline data characterizing chick growth and development under known feeding conditions are unavailable, so it is difficult to evaluate chick nutritional status as it relates to foraging conditions near breeding colonies. To address this need, we examined the growth and development of young Caspian Terns (Hydroprogne caspia), a cosmopolitan, generalist piscivore, reared in captivity and fed ad libitum and restricted (ca. one-third lower caloric intake) diets. Ad libitum-fed chicks grew at similar rates and achieved a similar size at fledging as previously documented for chicks in the wild and had energetic demands that closely matched allometric predictions. We identified three general characteristics of food-restricted Caspian Tern chicks compared to ad libitum chicks: (1) lower age-specific body mass, (2) lower age-specific skeletal and feather size, such as wing chord length, and (3) heightened levels of corticosterone in blood, both for baseline levels and in response to acute stress. Effects of diet restriction on feather growth (10-11% slower growth in diet-restricted chicks) were less pronounced than effects on structural growth (37-52% slower growth) and body mass (24% lower at fledging age), apparently due to preferential allocation of food resources to maintain plumage growth. Our results suggest that measurements of chick body mass and feather development (e.g., wing chord or primary length) or measurement of corticosterone levels in the blood would allow useful evaluation of the nutritional status of chicks reared in the wild and of food availability in the foraging range of adults. Such evaluations could also inform demography studies (e.g., predict future recruitment) and assist in evaluating designated piscivorous waterbird

  4. Dietary effects of chitosan and buckwheat ( Fagopyrum esculentum ...

    African Journals Online (AJOL)

    Fagopyrum esculentum) alone or in combination with chitosan, in broiler diets, on the growth, feed intake and serum lipid profile of broilers. Starting as 10-day olds, 144 Cobb 500 broiler chicks were fed six manually prepared diets containing ...

  5. Effect of egg storage duration and brooding temperatures on chick growth, intestine morphology and nutrient transporters.

    Science.gov (United States)

    Yalcin, S; Gursel, I; Bilgen, G; Horuluoglu, B H; Gucluer, G; Izzetoglu, G T

    2017-10-01

    The effects of egg storage duration (ESD) and brooding temperature (BT) on BW, intestine development and nutrient transporters of broiler chicks were investigated. A total of 396 chicks obtained from eggs stored at 18°C for 3 days (ESD3-18°C) or at 14°C for 14 days (ESD14-14°C) before incubation were exposed to three BTs. Temperatures were initially set at 32°C, 34°C and 30°C for control (BT-Cont), high (BT-High) and low (BT-Low) BTs, respectively. Brooding temperatures were decreased by 2°C each at days 2, 7, 14 and 21. Body weight was measured at the day of hatch, 2, 7, 14, 21, 28 and 42. Cloacal temperatures of broilers were recorded from 1 to 14 days. Intestinal morphology and gene expression levels of H+-dependent peptide transporter (PepT1) and Na-dependent glucose (SGLT1) were evaluated on the day of hatch and 14. Cloacal temperatures of chicks were affected by BTs from days 1 to 8, being the lowest for BT-Low chicks. BT-High resulted in the heaviest BWs at 7 days, especially for ESD14-14°C chicks. This result was consistent with longer villus and larger villus area of ESD14-14°C chicks at BT-High conditions. From 14 days to slaughter age, BT had no effect on broiler weight. ESD3-18°C chicks were heavier than ESD14-14°C chicks up to 28 days. The PepT1 and SGLT1 expression levels were significantly higher in ESD3-18°C chicks than ESD14-14°C on the day of hatch. There was significant egg storage by BT interaction for PepT1 and SGLT1 transporters at day 14. ESD14-14°C chicks had significantly higher expression of PepT1 and SGLT1 at BT-Low than those at BT-Cont. ESD14-14°C chicks upregulated PepT1 gene expression 1.15 and 1.57-fold at BT-High and BT-Low, respectively, compared with BT-Cont, whereas PepT1 expression was downregulated 0.67 and 0.62-fold in ESD3-18°C chicks at BT-High and BT-Low. These results indicated that pre-incubation egg storage conditions and BTs affected intestine morphology and PepT1 and SGLT1 nutrient transporters

  6. Responses of commercial broiler chicks to 60Co gamma-rays

    International Nuclear Information System (INIS)

    Shebaita, M.K.; Kamar, G.A.R.; El-Barkouky, E.E.M.

    1981-01-01

    A total of 360 chicks of Nichols line at one-day old were used in this study. Within each sex, the chicks were divided into three exposure treatments (non-irradiated, 400 rads and 800 rads). The source of irradiation was 60 Co gamma-rays. The results can be summarized as follows: Of the 360 chicks used in this study, only 10 chicks died during the experimental period. Radiation induced a reduction in body weight in both sexes during the growth period. Gamma-rays reduce the feed intake in both sexes. Moreover, the feed conversion ratio for the irradiated chicks was higher than that the non-irradiated birds. While red blood cells and hemoglobin content were higher in the irradiated chicks, white blood cells count and especially lymphocytes did not affect by 60 Co gamma-rays. Gamma-rays had no effect on carcass, bone, meat and giblets as fractions of body weight in both sexes. Moreover, meat water content decreased and meat fat content increased in irradiated chicks. The total length and the total weight of the GI tract decreased due to 60 Co gamma-rays. The response of the different regions of the GI tract to this rays is different. Moreover, the effect of this radiation on the GI tract is dependent upon the body weight irrespective of the sex. (orig./MG) [de

  7. In ovo exposure to omega-3 fatty acids does not enhance omega-3 long-chain polyunsaturated fatty acid metabolism in broiler chickens.

    Science.gov (United States)

    Kanakri, K; Carragher, J; Muhlhausler, B; Hughes, R; Gibson, R

    2017-10-01

    The content of omega-3 long-chain polyunsaturated fatty acids (n-3 LCPUFA) in chicken meat can be boosted by feeding broilers a diet containing α-linolenic acid (ALA, from flaxseed oil), some of which is converted by hepatic enzymes to n-3 LCPUFA. However, most of the accumulated n-3 polyunsaturated fatty acid (PUFA) in meat tissues is still in the form of ALA. Despite this, the levels of chicken diets are being enhanced by the inclusion of vegetable and marine sources of omega-3 fats. This study investigated whether the capacity of chicken for n-3 LCPUFA accumulation could be enhanced or inhibited by exposure to an increased supply of ALA or n-3 LCPUFA in ovo. Breeder hens were fed either flaxseed oil (High-ALA), fish oil (high n-3 LCPUFA) or tallow- (low n-3 PUFA, Control) based diets. The newly hatched chicks in each group were fed either the High-ALA or the Control diets until harvest at 42 days' post-hatch. The n-3 PUFA content of egg yolk and day-old chick meat closely matched the n-3 PUFA composition of the maternal diet. In contrast, the n-3 PUFA composition of breast and leg meat tissues of the 42-day-old offspring closely matched the diet fed post-hatch, with no significant effect of maternal diet. Indeed, there was an inhibition of n-3 LCPUFA accumulation in meat of the broilers from the maternal Fish-Oil diet group when fed the post-hatch High-ALA diet. Therefore, this approach is not valid to elevate n-3 LCPUFA in chicken meat.

  8. Haematological, biochemical and organ changes in broiler chickens fed varying levels of Morinda lucida (brimstone) leaf meal supplementation in the diets.

    Science.gov (United States)

    Lala, A O; Ajayi, O L; Okwelum, N; Oso, A O; Fakorede, T V; Adebayo, T A; Jagbojo, J E

    2018-06-01

    The aim of this study was to evaluate the effects of dietary supplementation of Morinda lucida leaf meal (MLLM) on the haematology, biochemical and organ changes of broiler chickens. One hundred and ninety-eight day-old Marshall broiler chicks were completely randomised into 6 treatments in a 3 × 2 factorial arrangement of three levels of M. lucida leaf meal supplementation (0, 0.1 and 0.2 g/kg) with or without medication. The treatment consisted of both negative (without MLLM and routine medication) and positive (containing no MLLM but with routine medication) control groups while each treatment was replicated thrice. MLLM-supplemented diets and routine medication decreased (p  0.05) on the liver, kidney, heart and gizzard. M. lucida leaf meal can be compared to routine medication for improved health status of broiler chickens. Dietary inclusion with 0.1 g/kg MLML combined with routine medication could be used in producing healthy and safe chickens.

  9. Effect of adding crushed Pimpinella anisum, Nigella sativa seeds and Thymus vulgaris mixture to antibiotics-free rations of vaccinated and non-vaccinated male broilers on growth performance, antibody titer and haematological profile

    Directory of Open Access Journals (Sweden)

    Mamoun Z. Athamneh

    2010-04-01

    Full Text Available This research explores an experimental study conducted to investigate the effect of crushed Pimpinella anisum (PA, Nigella sativa (NS seeds and Thymus vulgaris (TV mixture as a feed additive on growth performance and mortality rate (MR, selected antibodies titer (Ab’s and blood hematological profile of vaccinated and non-vaccinated Lohman male broiler chicks fed free-antibiotics ration. A total of 400 one-day old chicks were distributed into 16 groups (4 treatment x 4 replicates x 25chicks. The experiment lasted from one to 42 days of age. The statistical findings of this experiment prove that the use of medicinal plants mixture improves live body weight, body weight gain, feed conversion ratio and MR of vaccinated male broilers at 21 and 42 days of age. antibodies titer against infectious bronchitis and infectious bursal disease of non-vaccinated and vaccinated male broilers were significantly improved at 21 and 42 days as a result of the addition of medicinal plant mixture to the basal ration. Concerning Newcastle disease, the use of PA, NS and TV mixture did not reflect in any additional improvement of Ab's than vaccines did. The addition of medicinal plants mixture increases WBC's, RBC's, thrombocytes count and Hb concentration of vaccinated and non-vaccinated male broilers at 21 days of age. Meanwhile, heterophils, lymphocytes and monocytes of vaccinated male broilers (VMB were significantly improved by adding medicinal plant mixture to their basal diet. Moreover, at 42 days of age the use of PA, NS seeds and TV mixture indicate significant increase in total WBC’s, lymphocytes and monocytes and monocytes count of VMB and non-vaccinated male broiler (NVMB. No significant differences were noticed in RBC’s and Hct as a result of feeding crushed medicinal plants mixture.

  10. EFFECT OF SOAKED AND FERMENTED AFRICAN LOCUST BEAN (Parkia biglobosa SEED MEAL ON GROWTH PERFORMANCE, HAEMATOLOGICAL PROFILE AND NUTRIENT DIGESTIBILITY OF BROILER CHICKENS

    Directory of Open Access Journals (Sweden)

    M.S. Tamburawa

    2017-04-01

    Full Text Available African locust bean (Parkia biglobosa seed is rich in protein and has recently found its way into the feed industry. This research was conducted to determine the growth performance, haematological profile and nutrient digestibility by broiler chickens fed diets containing soaked and fermented African locust bean (Parkia biglobosa seed meal (SFALBSM. Five diets were formulated in which SFALSBM was included at graded levels of 0, 7.5, 15, 22.5 and 30% designated as T1, T2, T3, T4 and T5 respectively. Two hundred and twenty five (225 day old broiler chickens (Marshall Strain were fed these diets in a completely randomized design and each treatment was replicated three times with 15 birds per replicate. The experiment lasted 8 weeks (4week starter phase and 4week finisher phase. The results of performance of broiler chicks at starter phase showed there were differences (P0.05 on digestibility of dry matter, crude protein and ash. The crude fibre, ether extract and nitrogen free extract digestibility were affected by treatments (P<0.05. Birds fed T3 had highest crude fibre digestibility value (P<0.05. It was concluded that soaked and fermented African locust bean seed meal can be included in broiler chickens diets up to 15% dietary level at the starter phase and 22.5% at the finisher phase without any adverse effect on performance, haematological profile and nutrient digestibility.

  11. Application of genomic densitometry for calculating the relative population of Escherichia Coli in the intestine of broiler chicks

    Directory of Open Access Journals (Sweden)

    A.R Seidavi

    2009-05-01

    Full Text Available In this study, the densitometry technique for calculating of the relative population of Escherichia coli in various segments of the intestine of broiler chicks was evaluated. Following preparation of the intestinal contents, the process of extraction and purification of DNA from the contents of duodenum, jejunum, ileum and cecum was undertaken. A specific polymerase chain reaction (PCR using two pairs of primers was employed to detect Escherichia coli and total bacteria present in the gastrointestinal tract of the chicks. Specific bands of E.coli were obtained using densitometry and Gel Proc Analyzer software based on linear regression with extrapolation. E.coli populations at different ages were also determined in various segments of the gastrointestinal tract of the chicks. The Results of this experiment indicated that 0.000004%, 0.07%, 0.64% and 2.51% of total bacteria present in the duodenum, jejunum, ileum and cecum respectively consisted of E.coli. Also, E.coli constitutes 1.76, 0.01 and 0.80% of the total intestinal bacteria of chicks at 4, 14 and 30 days of age respectively. Furthermore, it was shown that at 4 days of age, 0.30, 2.05 and 3.97% of the total bacteria present in the jejunum, ileum and cecum respectively were from E.coli species and this bacteria was absent in the duodenum. At 14 days of age these figures were 0.000009%, 0.00011% and 0.08% respectively while at 30 days of age 0.00011%, 0.009% and 2.40% of all bacteria in the duodenum, ileum and cecum were E.coli species and this bacteria was absent in the jejunum. In conclusion, the densitometry method based on PCR results can be regarded as a useful tool for densitometry the relative population E.coli in the gastrointestinal tract of poultry.

  12. Broiler performance fed jatropha curcas seed meal detoxified by fermentation, physic and chemical treatments

    Directory of Open Access Journals (Sweden)

    Elizabeth Wina

    2010-10-01

    Full Text Available Utilization of jatropha seed meal as a feed ingredient is limited by the presence of several anti nutritive and toxic compounds in the seed meal. The aim of this research is to evaluate feeding of jatropha seed meal detoxified using fermentation by two fungi and rumen microbes (as biological detoxification and using a combination of chemical and physical treatments on broiler performance. One hundred seventy five chicks (7 days old were used and were divided into 5 treatments in 7 replications and each replication in one cage consisted of 5 chicks. R1 was control feed (K without jatropha seed meal., 2 R2 was feed with 4% of Jatropha seed meal fermented by Neurosphora sitophila (FNS, R3 was feed with 4% of Jatropha seed meal fermented by Aspergillus oryzae (FAS, R4 was feed with 4% of Jatropha seed meal fermented by rumen microbes and R5 with 4% of Jatropha seed meal treated by autoclaved, refluxed by hexane and soaked in methanol (OEHM. Treated feed was given for 14 days at the end of the feeding treatment, two chickens from each replication were slaughtered and organ weights were recorded. Body weight of chicken and feed conversion ratio were calculated. The rest of the chicken was fed commercial feed for the next 7 days (recovery periode. Chicken mortality was almost 0% but 1 chicken from FAS treatment died at the recovery period. Feed consumption was lower at fermented jatropha seed meal than control (K or OEHM, resulted in lower body weight of chicken. The abdomen fat weight and organ weights especially pancreas or spleen resulted from treatment with jatropha were much lower than that of K. In the recovery period, body weight of chicken in the OEHM treatment was almost similar from that of control chicken. In conclusion, biological detoxification on BBJ was able to reduce chicken mortality but could not improve the daily gain higher than control treatment. The best method to detoxify jatropha seed meal was the combination of physical and

  13. The effect of drying method temperature, collection method, and marker type on apparent ileal amino acid digestibility in 21-day-old broilers fed corn-soybean meal-barley based diet.

    Science.gov (United States)

    Olojede, O C; Ford, M J; Jacob, J P; Ao, T; Pescatore, A J; Adedokun, S A

    2018-06-01

    For accurate estimation of nutrient digestibility, an ideal drying and sampling method is required to preserve the quality of the digesta. A standard corn-soybean meal (corn-SBM) broiler starter diet was fed from d 0 to 10 before birds were placed on the experimental diets until d 21. One hundred and sixty-eight male Cobb 500 broiler chicks were used to evaluate the effect of two drying methods (freeze-dryer vs. forced air-oven) and two drying temperatures (40 vs. 55°C) (Exp 1), while ninety-six chicks were used to evaluate the effect of flushing and squeezing as well as marker types (titanium vs. chromium) on apparent ileal DM, N, Ca, P, and AA digestibility (Exp 2). There were seven (Exp 1) or eight (Exp 2) replicate cages per treatment with 6 birds/cage. Digesta from the distal two thirds of the ileum was obtained from birds following euthanasia on d 21 by squeezing (Exp 1) and squeezing or flushing (Exp 2). Samples collected were stored in the freezer at -20°C until they were either freeze-dried (FD) or oven-dried (OD) at 40 or 55°C. There were no interactions between the drying methods and drying temperatures (Exp 1) on apparent ileal DM, N, and AA digestibility. Met had the highest (92.3%) while Cys had the lowest (73.8%) digestibility value. In Exp 2, no interaction between sampling methods and marker types was observed. The effect of sampling methods was not significant except for Arg and Met where squeezing resulted in higher (P digestibility values. Furthermore, apparent ileal His, Ile, Cys, Ser, and Tyr digestibility tended to be higher (P digestibility. Likewise, marker type did not influence apparent ileal AA digestibility values.

  14. Effect of Poultry Litter Treatment (PLT) on death due to ascites in broilers.

    Science.gov (United States)

    Terzich, M; Quarles, C; Goodwin, M A; Brown, J

    1998-01-01

    The purposes of this study were to determine the effect of Poultry Litter Treatment (PLT) on levels of litter moisture, litter nitrogen, atmospheric ammonia, and death due to ascites. Data were collected from chicks raised in containment conditions that resembled commercial settings. The ascites death rate (5.9%) in broiler chicks on PLT-treated litter was significantly (chi 2 = 15.5, df = 1, P = 0.0001) lower than that (31.5%) in broiler chicks raised on untreated litter. Likewise, atmospheric ammonia levels in pens that had been treated with PLT were significantly (P litter moisture and litter nitrogen levels were not different (P > 0.05) among treatments at any sample interval.

  15. Response of broiler chickens to different dietary crude protein and feeding regimens

    Directory of Open Access Journals (Sweden)

    JO Oyedeji

    2005-09-01

    Full Text Available Five isocaloric (3200kcal/kg diets were used in an experiment designed to investigate the effects of dietary crude protein (CP and feeding regimens on broiler performance. Day-old broilers were randomly distributed into four groups using a completely randomized design. Each group was replicated three times with ten broiler chicks per replicate. The experiment lasted for eight weeks. Broilers in group 1 received 23% CP from 0 to 3 weeks, 20% CP from 3 to 6 weeks and 18% CP from 6 to 8 weeks, while broilers in group 2 received 23% CP between 0 and 6 weeks and 18% CP between 6 and 8 weeks. Besides, broilers in group 3 were fed 23% CP from 0 to 4 weeks and 16% CP from 4 to 8 weeks, whereas group 4 was given 18% CP from 0 to weeks. Water was supplied ad libitum for broilers in the different dietary groups. A metabolic trial was carried out on the third week of the experiment using a total collection method. Proximate analyses of diets and faecal samples were performed according to the methods outlined by the Association Of the Official Analytical Chemists. Results at market age showed that broiler performance with respect to feed intake, weight gain, feed to gain ratio and water intake were not significantly influenced by CP regimens (p>0.05. Furthermore, CP regimens did not significantly influence broilers liveability (p>0.05. Protein retention, fat utilization and available fiber were not significantly influenced among treatments (p> 0.05. Economic data showed that cost to benefit ratio of producing broilers was comparable among broilers for all CP regimens used in this trial (p>0.05. It was concluded that a single diet of 18% CP and 3200kcal/kg metabolizable energy would be most suitable and convenient for farmers who are engaged in on-farm feed production for broilers as compared with the standard feeding regimens of broiler starter and broiler finisher diets.

  16. Growth performance and immunity status of starter broiler birds ...

    African Journals Online (AJOL)

    This study was conducted to investigate the effects of feeding diets containing Neem Leaf Meal (NLM), Garlic Meal (GM) and their combinations (NLM + GM) on growth performance and serum parameters of starter broiler birds. A total of 180 day-old Cobb broiler chickens were divided into twelve groups of fifteen chicks with ...

  17. Browse Title Index

    African Journals Online (AJOL)

    Items 201 - 250 of 350 ... Vol 5, No 3 (2009), Lactic Acid Probiotic Influence on Salmonella Enteritidis ... Vol 6, No 2 (2010), Microbiological Quality Assessment of Ready-To-Eat Kilishi Sold in ... of Broiler Finisher Birds Fed Varying Dietary Levels of Pineapple ... carcass characteristics of starter broiler chicks fed high fiber rice ...

  18. Effects of dietary physical form and dietary inclusion of probiotic and ...

    African Journals Online (AJOL)

    Hosseini

    2017-09-27

    Sep 27, 2017 ... The WG of the broiler chicks fed the pelleted diets containing the enzyme ..... growth performance of broilers synergistically by increasing muscle protein .... galactooligosaccharides on TNF-α mediated inflammation in human ...

  19. Effects of glycerol on the metabolism of broilers fed increasing glycerine levels

    OpenAIRE

    Romano,GG; Menten,JFM; Freitas,LW; Lima,MB; Pereira,R; Zavarize,KC; Dias,CTS

    2014-01-01

    This study evaluated the metabolic response of broilers fed diets containing increasing crude glycerine levels in two bioassays. Birds were house in metabolic cages, and were distributed according to a completely randomized experimental design with five treatments of 4 replicates each (1st assay: 5 birds/ cage; 2nd assay: 1-20 days = 8 birds/ cage, and 21-42 days = 4 birds/cage). Treatments consisted of a control diet based on corn and soybean meal, and four other diets containing 2.5%, 5.0%,...

  20. Effect of dietary supplementation with Lactobacillus acidophilus D2/CSL (CECT 4529) on caecum microbioma and productive performance in broiler chickens

    OpenAIRE

    De Cesare, Alessandra; Sirri, Federico; Manfreda, Gerardo; Moniaci, Paola; Giardini, Alberto; Zampiga, Marco; Meluzzi, Adele

    2017-01-01

    This study examines the effects of the dietary supplementation with Lactobacillus acidophilus D2/CSL (CECT 4529) (LA) on productive performances, incidence of foot pad dermatitis and caecum microbioma in broiler chickens. A total of 1,100 one-day old male Ross 308 chicks were divided into 2 groups of 16 replicates with 25 birds each and reared from 1-41 d. One group was fed a basal diet (CON) and the other group the same diet supplemented with LA. Caecum contents were collected from 4 selecte...

  1. Effect of -based Direct-fed Microbial on Performance, Nutrient Utilization, Intestinal Morphology and Cecal Microflora in Broiler Chickens

    Directory of Open Access Journals (Sweden)

    Xinjian Lei

    2015-02-01

    Full Text Available The present study was conducted to evaluate the effect of the dietary supplementation of Bacillus amyloliquefaciens-based direct-fed microbial (DFM on growth performance, nutrient utilization, intestinal morphology and cecal microflora in broiler chickens. A total of two hundred and eighty eight 1-d-old Arbor Acres male broilers were randomly allocated to one of four experimental treatments in a completely randomized design. Each treatment was fed to eight replicate cages, with nine birds per cage. Dietary treatments were composed of an antibiotic-free basal diet (control, and the basal diet supplemented with either 15 mg/kg of virginiamycin as antibiotic growth promoter (AGP, 30 mg/kg of Bacillus amyloliquefaciens-based DFM (DFM 30 or 60 mg/kg of Bacillus amyloliquefaciens-based DFM (DFM 60. Experimental diets were fed in two phases: starter (d 1 to 21 and finisher (d 22 to 42. Growth performance, nutrient utilization, morphological parameters of the small intestine and cecal microbial populations were measured at the end of the starter (d 21 and finisher (d 42 phases. During the starter phase, DFM and virginiamycin supplementation improved the feed conversion ratio (FCR; p<0.01 compared with the control group. For the finisher phase and the overall experiment (d 1 to 42 broilers fed diets with the DFM had better body weight gain (BWG and FCR than that of control (p<0.05. Supplementation of virginiamycin and DFM significantly increased the total tract apparent digestibility of crude protein (CP, dry matter (DM and gross energy during both starter and finisher phases (p<0.05 compared with the control group. On d 21, villus height, crypt depth and villus height to crypt depth ratio of duodenum, jejunum, and ileum were significantly increased for the birds fed with the DFM diets as compared with the control group (p<0.05. The DFM 30, DFM 60, and AGP groups decreased the Escherichia coli population in cecum at d 21 and d 42 compared with control

  2. Effect of different types of litter material for rearing broilers.

    Science.gov (United States)

    Swain, B K; Sundaram, R N

    2000-07-01

    1. Coir dust was evaluated as broiler litter in comparison with sawdust and rice husk using 135 commercial broilers. Forty-five broiler chicks were reared to 42 d on a 50 mm layer of each of these litters. 2. Birds reared on coir dust showed no difference in food consumption, body weight gain, food conversion efficiency production number and survivability in comparison to those reared on saw dust and rice husk. 3. It was concluded that coir dust is suitable as broiler litter when cheaply available.

  3. The pecking, resting and feeding behaviour of four broiler strains in ...

    African Journals Online (AJOL)

    The pecking, resting and feeding behaviour of four broiler strains in a humid tropical environment. ... Nigerian Journal of Animal Production ... An experiment to determine the pecking, resting and feeding behaviour of four strains of broilers (Anak, Hubbard, Lohman, and Hybro) was conducted with 120 day old chicks.

  4. Effect of two microbial phytase preparations on phosphorus utilisation in broilers fed maize-soybean meal based diets

    Directory of Open Access Journals (Sweden)

    J. VALAJA

    2008-12-01

    Full Text Available The present study was carried out to determine the effect of two microbial phytases, Aspergillus niger (FINASEâ FP-500, 291 PU (phytase units/g and Trichoderma reesei phytase (FINASEâ P, 5880 PU/g on phosphorus (P and calcium (Ca utilisation and ileal P and Ca digestibility in broiler chickens fed diets based on maize and soybean meal. A total of 96 Ross broiler chickens housed four birds to a cage were used. Four dietary treatments consisted of a positive control supplemented with dicalcium phosphate (17 g/kg, a negative control without inorganic P, basal diet without inorganic P supplemented with Aspergillus niger phytase (2.6 g/kg and basal diet without inorganic P supplemented with Trichoderma reesei phytase (0.13 g/kg. Both phytases provided 750 PU/kg feed. P retention per unit intake was lowest and P excretion highest in birds fed the positive control diet with inorganic P (P

  5. Sodium butyrate improved performance while modulating the cecal microbiota and regulating the expression of intestinal immune-related genes of broiler chickens.

    Science.gov (United States)

    Bortoluzzi, C; Pedroso, A A; Mallo, J J; Puyalto, M; Kim, W K; Applegate, T J

    2017-09-01

    This study evaluated the effect of sodium butyrate (SB) on performance, expression of immune-related genes in the cecal tonsils, and cecal microbiota of broiler chickens when dietary energy and amino acids concentrations were reduced. Day-old male Ross 708 broiler chicks were fed dietary treatments in a 3 × 2 factorial design (8 pens per treatment) with 3 dietary formulations (control diet; reduction of 2.3% of amino acids and 60 kcal/kg; and reduction of 4.6% of amino acids and 120 kcal/kg) with or without the inclusion of 0.1% of SB. Feed intake (FI), body weight gain (BW gain), and feed conversion ratio (FCR) were recorded until 28 d of age. From 14 to 28 d, there was an interaction of nutrient density by SB (P = 0.003) wherein BW gain of birds fed SB was impaired less by the energy/amino acids reduction than unsupplemented birds. A similar result was obtained from 1 to 28 d (P = 0.004). No interaction (P density by SB was observed for FCR. Nutritional density of the diets and SB modified the structure, composition, and predicted function of the cecal microbiota. The nutritionally reduced diet altered the imputed function performed by the microbiota and the SB supplementation reduced these variations, keeping the microbial function similar to that observed in chickens fed a control diet. The frequency of bacterial species presenting the butyryl-CoA: acetate CoA-transferase gene increased in the microbiota of chickens fed a nutritionally reduced diet without SB supplementation, and was not changed by nutrient density of the diet when supplemented with SB (interaction; P = 0.01). SB modulated the expression of immune related genes in the cecal tonsils; wherein SB upregulated the expression of A20 in broilers fed control diets (P broilers fed nutritionally reduced diets, partially by modulating the cecal microbiota and exerting immune-modulatory effects. © 2017 Poultry Science Association Inc.

  6. Response of broiler chickens to feed supplemented with claybased ...

    African Journals Online (AJOL)

    An eight week study was carried out to evaluate the effects of MFeed®, a nanotechnology growth promoter as feed additive in the diets of broiler chickens. Two hundred, day old Marshal broiler chicks were allotted in a completely randomised design to five dietary treatments supplemented with MFeed® at 0, 1, 2, 3, 4 and 5 ...

  7. The addition of charcoals to broiler diets did not alter the recovery of Salmonella Typhimurium during grow-out.

    Science.gov (United States)

    Wilson, K M; Bourassa, D V; Davis, A J; Freeman, M E; Buhr, R J

    2016-03-01

    Two experiments evaluated prebiotics added to feed on the recovery of Salmonella in broilers during grow-out and processing. In Experiment 1, "seeder" chicks were inoculated with Salmonella Typhimurium and placed with penmates. Treatments were: basal control diet, added 0.3% bamboo charcoal, 0.6% bamboo charcoal, or 0.12% Aromabiotic (medium chain fatty acids). The ceca from seeders and penmates were sampled to confirm Salmonella colonization at 3, 4, and 6 wk, and pen litter was sampled weekly. At 3 wk, charcoal fed chicks had significantly lower cecal recovery (37% lower) of Salmonella via direct plating but no differences at wk 4 or 6. At 6 wk, broilers fed Aromabiotic had no recovery of Salmonella from ceca with direct plating and significantly, 18%, lower recovery with enrichment. In Experiment 2, the treatments were: basal control diet, added 0.3% bamboo charcoal, 0.3% activated bamboo charcoal, or 0.3% pine charcoal. At placement, 2 seeders were challenged with Salmonella and commingled with penmates and ceca sampled at 1 and 2 wk, and ceca from 5 penmates/pen at 3 to 6 wk. Weekly, the pH of the crop and duodenum was measured from 1 penmate/pen and the litter surface sampled. At the end of grow-out broilers were processed. Results showed that penmates had colonized at 1 and 2 wk. Cecal Salmonella showed no differences except at 4 wk, when activated bamboo charcoal had a 18% lower recovery of Salmonella (enrichment) compared to the control (88%). Similar to Experiment 1, the recovery of Salmonella from the litter was not significantly different among treatments, however an overall decrease in recovery by 4 wk with direct plating reoccurred. The pH of the duodenum and the crop were not different among treatments. Crop pH (6.0) for all treatments were significantly higher at wk 1 compared to wk 2 to 6. Charcoals had minimal effect on Salmonella recovery in the ceca, but following defeathering, broilers fed charcoals had significantly lower Salmonella

  8. Effects of prebiotics, probiotics, and their combination on growth performance, small intestine morphology, and resident Lactobacillus of male broilers.

    Science.gov (United States)

    Wang, X; Farnell, Y Z; Peebles, E D; Kiess, A S; Wamsley, K G S; Zhai, W

    2016-06-01

    Effects of commercial antimicrobials and the individual and combinational use of commercial prebiotics and probiotics in feed from d zero to 41 on the growth performance, small intestine size, jejunal morphology, and ileal resident bacteria population of broiler chickens were determined. A total of 1,040 one-day-old male Ross × Ross 708 broilers were randomly distributed to 80 floor pens (5 treatments, 16 replications per treatment, 13 chicks per pen). Five dietary treatments were employed: 1) a corn soybean-meal basal diet (served as a negative control diet, NC); 2) a basal diet supplemented with a commercial prebiotic product (Pre); 3) a basal diet supplemented with a probiotic product containing Bacillus subtilis spores (Pro); 4) a basal diet supplemented with both prebiotic and probiotic products (Pre + Pro); and 5) a basal diet supplemented with commercial antimicrobials (served as a positive control diet, PC). At d 14, Pre diets improved the relative level of Lactobacillus in ileal mucosa as compared to NC, Pro, or PC diets (P = 0.045) without improving broiler BW. Broilers fed PC diets exhibited the highest BW gain from d 15 to 27, the lowest duodenum, jejunum, and ileum relative weights as percentage of BW at d 27, and the highest breast weight at d 42 (P = 0.026, 0.035, 0.002, 0.025, and 0.035, respectively). Broilers fed Pro or Pre + Pro diets exhibited higher BW gain from d 28 to 41 (P = 0.005) and higher overall BW gain from d zero to 41 (P = 0.039) than those fed other diets. Dietary treatments did not affect jejunal morphology or ileal resident Escherichia coli level at any age. From our results, including spores of Bacillus subtilis in feed may stimulate growth at a later age and may facilitate broilers in reaching their target weight sooner. Therefore, probiotics are recommended as potential alternatives to antimicrobials in chicken diets, especially in grower and finisher feed. © 2016 Poultry Science Association Inc.

  9. Effects of folic acid supplementation on the performance of broiler ...

    African Journals Online (AJOL)

    Sixty (60) day- old broiler chicks were used to investigate the effects of graded levels of folic acid supplementation on the performance of broiler chickens. The study was carried out using complete randomized design. Commercial starter and finisher diets were used for the experiment which lasted for eight weeks. The folic ...

  10. Transmission of Salmonella to broilers by contaminated larval and adult lesser mealworms, Alphitobius diaperinus (Coleoptera: Tenebrionidae).

    Science.gov (United States)

    Roche, A J; Cox, N A; Richardson, L J; Buhr, R J; Cason, J A; Fairchild, B D; Hinkle, N C

    2009-01-01

    The ability of the lesser mealworm, Alphitobius diaperinus (Panzer), commonly known as the darkling beetle, to transmit marker Salmonella Typhimurium to day-of-hatch broiler chicks was evaluated, as well as the spread to nonchallenged pen mates. In trial 1, day-of-hatch chicks were orally gavaged with 4 larval or 4 adult beetles that had been exposed to marker Salmonella-inoculated feed for 72 h. In addition, chicks were gavaged with the marker Salmonella in saline solution. These chicks were then placed into pens to serve as challenged broilers. In trial 2, all pens received 2 challenged chicks that were gavaged with larvae or beetles that had been exposed to marker Salmonella-inoculated feed for 24 h and then removed from the inoculated feed for a period of 7 d. At 3 wk of age, cecal samples from the marker Salmonella-challenged broilers and from 5 pen mates in trial 1, or 10 pen mates in trial 2, were evaluated for the presence of the marker Salmonella in their ceca, and at 6 wk of age, all remaining pen mates were sampled. To monitor the presence of the marker Salmonella within pens, stepped-on drag swab litter samples were taken weekly. For the Salmonella-saline pens, 29 to 33% of the broilers that had been challenged and 10 to 55% of the pen mates were positive at 3 wk of age, and only 2 to 6% had positive ceca at 6 wk. For the pens challenged with adult beetles, 0 to 57% of the challenged broilers and 20 to 40% of the pen mates had positive ceca at 3 wk, and 4 to 7% were positive at 6 wk. The pens challenged with larvae had the greatest percentage of marker Salmonella-positive broilers; 25 to 33% of the challenged broilers and 45 to 58% of pen mates were positive at 3 wk, and 11 to 27% were positive at 6 wk. These results demonstrated that ingestion of larval or adult beetles contaminated with a marker Salmonella could be a significant vector for transmission to broilers.

  11. Responses of commercial broiler chicks to /sup 60/Co gamma-rays

    Energy Technology Data Exchange (ETDEWEB)

    Shebaita, M.K.; Kamar, G.A.R.; El-Barkouky, E.E.M.

    1981-12-01

    A total of 360 chicks of Nichols line at one-day old were used in this study. Within each sex, the chicks were divided into three exposure treatments (non-irradiated, 400 rads and 800 rads). The source of irradiation was /sup 60/Co gamma rays. The results can be summarized as follows: Of the 360 chicks used in this study, only 10 chicks died during the experimental period. Radiation induced a reduction in body weight in both sexes during the growth period. Gamma rays reduce the feed intake in both sexes. Moreover, the feed conversion ratio for the irradiated chicks was higher than that the non-irradiated birds. While red blood cells and hemoglobin content were higher in the irradiated chicks, white blood cells count and especially lymphocytes were not affected by /sup 60/Co gamma rays. Gamma rays had no effect on carcass, bone, meat and giblets as fractions of body weight in both sexes. Moreover, meat water content decreased and meat fat content increased in irradiated chicks. The total length and the total weight of the GI tract decreased due to /sup 60/Co gamma rays. The response of the different regions of the GI tract to these rays is different. Moreover, the effect of this radiation on the GI tract is dependent upon the body weight irrespective of the sex.

  12. Performance and economy of production of broilers fed Siam weed (Chromolaena odorata) leaf meal (SWLM).

    Science.gov (United States)

    Jiwuba, P C; Ogbuewu, I P; Nwachukwuguru, K

    2018-03-06

    A 49-day feeding trial was conducted to determine the effect of Siam weed leaf meal (SWLM)-based diets on growth, organ, and carcass weight characteristics and economics of production of broilers. Proximate biochemical composition of SWLM and the appropriate inclusion level of SWLM for optimum productivity were also determined. Ninety-six-day-old Chi broiler chicks were randomly divided into 4 groups of 24 birds, and each group replicated three times. Each group was assigned to one experimental diet in a completely randomized design marked T1 (0%), T2 (4%), T3 (8%), and T4 (12%). Proximate biochemical results revealed that SWLM is rich in protein (20.52%) and minerals (9.29%). Birds on diets T1 and T2 had similar (p > 0.05) final live weights (FLW), which were higher (p  0.05) differences in the dressing percentage, but there were significant differences in the carcass weight between birds on diet T4 and the other three diets. Similarly, pancreas and spleen had similar (p > 0.05) weights across the treatments. There was significant difference (p < 0.05) between birds on diets T1 and T4 in gizzard weight. Cost-benefit ratio was influenced (p < 0.05) with T4 birds having better income of US$1 per US$10.18 invested. It is concluded that SWLM is rich in essential nutrients and therefore suitable for inclusion in broiler diets at level not beyond 2% for best final live weight, daily weight gain, and thigh weight.

  13. Broilers fed dietary vitamins harbor higher diversity of cecal bacteria and higher ratio of Clostridium, Faecalibacterium, and Lactobacillus than broilers with no dietary vitamins revealed by 16S rRNA gene clone libraries.

    Science.gov (United States)

    Luo, Yu-heng; Peng, Huan-wei; Wright, André-Denis G; Bai, Shi-ping; Ding, Xue-mei; Zeng, Qiu-feng; Li, Hua; Zheng, Ping; Su, Zhuo-wei; Cui, Ren-yong; Zhang, Ke-ying

    2013-09-01

    Research on the interaction between dietary vitamins and intestinal bacteria is poorly understood. To investigate the effect of dietary vitamins on the cecal bacterial communities, 2 bacterial 16S rRNA gene clone libraries were constructed from pooled PCR products obtained from the cecal digesta of 28-d broilers fed diets with vitamins (V) at the NRC level or with no vitamins (NV). The results showed that BW gain and average feed intake of V broilers was significantly higher (P vitamins can increase the ratio of facultative pathogenic bacteria and decrease the diversity of bacteria in the cecum of broilers. Our results provide new leads for further investigations on the interaction between dietary vitamin additives and the gut health of broilers.

  14. Effects of L-thyroxine on plasma biochemistry in broiler chicks (Gallus gallus)

    International Nuclear Information System (INIS)

    Chaudhry, M.A.; Siddiqui, Z.H.; Imran, M.; Ahsan, M.

    2009-01-01

    The effect of a single intraperitoneal injection of a low dose (1 mu g/g) and a high dose (4 mu /g) of thyroxine (T 4) were investigated with time on plasma metabolites in broiler (Hubbard) chicks. Plasma total proteins, albumin fraction, glucose and triglyceride levels were decreased significantly in both experimental groups. The plasma cholesterol values remained almost comparable with controls during the early stages of the experiment but after 72 hours of the experiment higher dose of T 4 resulted in a significant (p<0.01) decrease followed by significant (p<0.05) increase in the same experimental group. The plasma albumin fraction decreased significantly in animals injected with higher dose of T/sub 4/ after 24 hours and the same trend continue even after 72 hour of the experiment. Plasma uric acid values were significantly decreased in group administered with lower dose of T/sub 4/ after 12 hours of the post injection time. However in animals administered with higher dose of thyroid hormone reflected a significant increase in plasma uric acid levels after 12 hours of the experiment and its levels were significantly decreased after 48 hours. The results are discussed in the light of previous reports of thyroid hormones and their physiological implications in different vertebrate groups. (author)

  15. Skeletal development and performance of broilers

    International Nuclear Information System (INIS)

    El-Deeb, M.A.

    1989-01-01

    The effect of high dietary copper supplementation has been shown to promote growth rate. The results of the experiments reported herein indicated that copper supplementation of 100 ppm with zinc at the requirement level had no effect on growth rate or bone mineralization. However, when various levels of copper and zinc were added to the diet, significant changes in bone mineralization and liver zinc and copper concentrations occurred. In further studies, the effect of dietary supplementation with vitamin D 3 or its metabolite 1,25(OH) 2 D 3 on the incidence of tibial dyschondroplasia and related bone abnormalities in broiler chicks was investigated. The results indicated that feeding 1,25(OH) 2 D 3 at 5ug/Kg diet as the sole source of vitamin D 3 steroid was sufficient to maintain a calcium homeostasis in the chicks. The effect of growth hormone (GH) injections on the metabolism of tibial epiphyseal growth plant (EGP) in broiler chicks either genetically susceptible or resistant to tibial dyschondroplasia (HTD and LTD respectively) was investigated. GH injection increased 3 H-thymidine uptake, indicating a direct effect of GH on cell replication in EGP

  16. Effects of time of change from broiler starter to broiler finisher diet on ...

    African Journals Online (AJOL)

    A study was conducted to determine the effect of time of change from broiler starter ration (BSR) to broiler finisher ration (BFR) on growth performance and economy of gain of meat- type chickens. Six hundred Abor Acres broiler chickens were used in an 8-week study in which same starter and finisher diets were fed but at ...

  17. Rape seed glucosinolate: radiation inactivation and physiological performance of broiler fed irradiated rapeseed meal

    International Nuclear Information System (INIS)

    Farag, M.Diaa El-Din H.

    1994-01-01

    Rape seeds meal (RSM) is a high quality protein supplement suitable for all classes of livestock. The major area of concern in animal nutrition has been glucosinolates and their derivative products which cause depressed performance in poultry or may be even toxic. Therefore, these substances must be removed or inactivated before the meal can be used as potential protein source for food or feed. I the current study, RSM has been used to test whether gamma radiation processing can inactivate glucosinolates as a step towards detoxication. Samples were exposed to gamma rays of 10, 50, 100 and 250 kGy. Approximated analysis showed that RSM was not affected by irradiation processing up to 250 kGy. However, the crude fiber content decreased at the highest dose while at doses of 10, 50 100 and 250 kGy the available lysine decreased by 6.76%, 9.46%, 17.84% and 22.43%, respectively. Radiation processing at 250 kGy significantly inactivated glucosinolate by 85% from its initial value. In a 8-week chick-feeding study, raw and irradiated RSM were applied at 30%. The diets containing raw and irradiated (at 10, 50 and 100 kGy) RSM had somewhat low growth and thyroid, liver and kidney enlargement compared to the basal control group. No significant difference was observed between chicks fed on RSM irradiated at 250 kGy and those fed on basal diet. No significant differences were observed in the serum protein, albumin, GPT, uric acid, creatine and basal diet groups. Those kept on raw and irradiated at 10, 50 and 100 kGy RSM had higher GOT than those kept on irradiated at 250 kGy RSM and basal diet. Radiation treatment of RSM up to 250 kGy improved its nutritional quality by decreasing the glucosinolate and consequently maintained the chicks in a better health condition. (author)

  18. ( Rosa damascena Mill.) dreg: an alternative litter material in broiler ...

    African Journals Online (AJOL)

    The present study was carried out to determine the effects of using dried rose dreg (DRD) as an alternative litter material for broiler performance and microbiological characteristics of litter. A total of 225 day-old broiler chicks was raised on pine wood shavings (PS), DRD and PS+DRD until 42 days. The effects of litter ...

  19. EFFECTS OF HIGHER LEVELS OF CHROMIUM AND COPPER ON SOME HAEMATOLOGICAL PARAMETERS AND SERUM PROTEINS IN BROILERS

    Directory of Open Access Journals (Sweden)

    M. Tariq Javed, F, Ahmad. N, Z, Rafique1 and M, Bashir

    2003-01-01

    Full Text Available Effects of higher levels of chromium alone and in combination with copper were investigated in broiler chicks divided into seven equal groups viz. A, B, C, D, E, F and G. Group G served as control receiving no treatment. Groups A, B and F received chromium chloride at the rate of 2 g/kg and nicotinic acid 150 mg/kg feed while C, D and F received chromium chloride 8 g/kg and nicotinic acid 150mg/kg. Broilers of groups A and C received copper sulfate at the rate of 200 mg/kg while groups Band D 400 mg/kg feed. Haematological parameters studied revealed non-significant difference between treatment groups and control in haemoglobin concentration and total erythrocyte counts. However, only at 4th week, lower PCV was observed in birds fed higher levels of chromium chloride alone. Increase in TLC was observed in birds fed low chromium alone or' with low levels of copper. Results of serum proteins including total protein, albumin and globulin during first three weeks showed significantly or relatively lower values in treatment groups than control. Serum globulins generally revealed non-significant difference between treatment groups and control.

  20. Effects of Dietary Supplemental Vitamins and Periods of Administration on Growth Performance and Antibody Titre of Broiler Chickens Vaccinated against Newcastle Disease

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    Odutayo, O. J.

    2016-12-01

    Full Text Available This study investigated the effects of supplemental vitamins and varying administration periods on growth performance and antibody titre of broiler chickens vaccinated against Newcastle Disease (ND. A total of 300 unvaccinated against ND Arbor Acre day-old chicks were used for the study for 8 wk. Birds were brooded together on day 1 of age, and 30 chicks were selected randomly for evaluating the maternally derived antibody titre against ND. At 2 days of age, the remaining 270 chicks were divided based on weight equalization into 9 treatment groups and replicated thrice. The 9 treatments consisted of a factorial arrangement of 4 supplemental vitamins (A, C, E and combination of A, C, E and 2 periods of administration (3 days pre- and post-ND vaccinations with a control. The birds were managed intensively throughout the experimental period, ND vaccines were administered on the 5th (i/o and 24th (Lasota day of age, respectively. Supplemental combined vitamins A, C and E at 0.15, 16.67 and 3.03 mg/kg, respectively, resulted in higher (P < 0.05 final body weight of 1785.00 g/bird and better feed conversion ratio (FCR of 2.89. Also, birds fed vitamin A supplemented diet 3 d pre-i/o vaccine had higher (p<0.05 serum antibody titre (75.20 against ND while higher (p<0.05 serum antibody titre (741.33 was also obtained in birds fed diet supplemented with vitamin E 3 d post-Lasota vaccination. Conclusively, broiler chickens diets can be supplemented with combined vitamins A, C, and E for better growth performance measured as final body weight and FCR, in addition, vitamins A (0.45mg/kg and E (9.1mg/kg dietary supplementation at 3 d pre-i/o and 3 d post-Lasota vaccines, respectively, can be adopted for improved antibody production.

  1. The use of refused tea as litter material for broiler chickens.

    Science.gov (United States)

    Atapattu, N S B M; Wickramasinghe, K P

    2007-05-01

    A completely randomized design experiment was conducted to determine the suitability of refused tea (RT) as a litter material for broiler chickens. Physiochemical properties of RT were compared with paddy husk (PH). Subsequently, broilers were raised on RT- or PH-based litter to compare the performances and litter qualities. Twenty-day-old broiler chicks (n = 150) were randomly allocated into 6 deep litter pens so that each treatment had 3 replicates. Chicks received 0.8 ft(2) of floor spacing until d 28 and 1.3 ft(2) thereafter. Each cage had a feeder and a drinker. Litter materials and litter samples taken on 28, 35, and 39 d were analyzed for bulk density, moisture, ash, and N. Chick mortality was low (1.3%) and similar on 2 types of litters. Live weights on d 28, 35, 39, and weight gains, feed intakes, dressing percentages, and feed conversion ratios were not affected by the type of litter material. The bulk density, moisture level, and pH of the RT were comparable with PH. Even though the water-holding capacity of PH (213%) was significantly higher (P litter had around 10% units higher moisture level than PH litter. By d 39, the moisture content of the RT litter was (48%) significantly higher (P = 0.05) than PH litter (37%). The N contents of RT litter were higher (P litter material for broilers. A higher N content in RT-based spent broiler litter would make it be a better organic fertilizer and ruminant feed compared with PH-based litter.

  2. Effects of methionine deficiencies on plasma levels of thyroid hormones, insulin-like growth factors-I and -II, liver and body weights, and feed intake in growing chickens.

    Science.gov (United States)

    Carew, L B; McMurtry, J P; Alster, F A

    2003-12-01

    We showed previously that Met deficiency at 0.25% of the diet causes elevations in plasma triiodothyronine (T3) in broilers. In the present study, plasma levels of thyroid hormones as well as insulin-like growth factors (IGF)-I and -II were measured in chicks fed 3 deficient levels of total Met. Control (0.5%) and Met-deficient diets (0.4, 0.3, and 0.2%) were fed to male broilers from 8 to 22 d of age. Additional groups of control chicks were pair-fed with the Met-deficient ones. Chicks receiving 0.4% Met increased feed intake by 10% with no significant change in body weight. The more severe Met deficiencies of 0.3 and 0.2% caused graded reductions in feed intake and weight gain. However, corresponding pair-fed control chicks were significantly heavier. These changes suggest more marked alterations in metabolic processes with 0.3 and 0.2% Met than with 0.4% Met. Liver weights were heavier in chicks fed 0.3 and 0.2% Met but not 0.4%. Plasma T3 was higher in all deficient chicks compared with the free-fed control, which was significant only with 0.3% Met. However, with 0.3 and 0.2% Met, plasma T3 was significantly elevated compared to pair-fed controls. Plasma thyroxine (T4) was lower in all deficient groups, which was significant only with 0.2% Met, whereas no significant differences occurred between deficient chicks and their pair-fed controls. Plasma IGF-I levels were not significantly different, but they were consistently lower in deficient chicks and deserve further study. Plasma IGF-II was significantly less in chicks fed 0.2% Met compared to pair-fed controls suggesting that Met deficiency interferes with IGF-II metabolism. We concluded that a deficit of dietary Met altered plasma T3 and IGF-II levels, but the effect was dependent on the degree of deficiency.

  3. Direct-fed microbials in broiler chickens challenged with Eimeria maxima or raised on Clostridium spp.-contaminated used litter

    Science.gov (United States)

    Dietary direct-fed microbials (DFMs) influence the composition of gut microbiota and enhance gut health in broiler chickens. Increasing scientific data have been gathered to show that gut microbiota plays an important role in the development of the immune system and the maintenance of homeostasis wi...

  4. Roasted full-fat kidney bean (Phaseolus vulgaris L.) and soyabeans (Glycine max) meals in broiler chicken diet.

    Science.gov (United States)

    Poné, D K; Fomunyam, R T

    2004-07-01

    An 8-week experiment was carried out to evaluate the performance of broiler chickens fed on diets containing either roasted full-fat soyabean meal (RSBM), roasted full-fat kidney bean meal (RKBM), cottonseed cake meal (CSM) or soyabean meal (SBM). A total of 240 unsexed, day-old-chicks were randomly assigned to four treatments so that there were 10 chicks per experimental unit in a randomized complete block design with three blocks and two replicates per block. Cumulative feed intake was depressed (p 0.05) by treatment effects. Feed cost efficiency (CFA Franc (Fcfa) per kilogram live chicken) showed that the SBM-based diet (494 Fcfa) and RKBM-based diet (493 Fcfa) were more expensive (pRoasting soyabeans in place of imported SBM allowed farmers to save about 19% for each CFA franc spent on a kilogram of live bird at 8 weeks of age.

  5. Influence of combinations of fenugreek, garlic, and black pepper powder on production traits of the broilers

    Directory of Open Access Journals (Sweden)

    A. Kirubakaran

    2016-05-01

    Full Text Available Aim: To study the effects of combinations of fenugreek (Trigonella foenum-graecum L., garlic (Allium sativum, and black pepper (Piper nigrum powder supplementation on production traits of broiler chickens. Materials and Methods: A total of 288 commercial broiler chicks were randomly assigned to 1-9 groups with 4 replicates each. An experiment was conducted in broilers with different feed formulations; control feed, with no added fenugreek, garlic, and black pepper powder; and 8 treatment groups receiving feed supplemented with different combinations of fenugreek, garlic, and black pepper powder. The individual broilers’ body weight and feed consumption were recorded and calculate the body weight gain and feed conversion ratio (FCR. Results: Broiler’s weight gain and FCR were significantly higher in groups receiving feed supplemented with garlic and black pepper powder combinations (p<0.01. Cumulative feed consumption was significantly higher in groups receiving feed supplemented with garlic and black pepper powder combinations (p<0.01. Conclusion: The combination of garlic and black pepper powder supplemented broiler feed fed groups showed higher production performance. The 5 g/kg garlic powder+1 g/kg black pepper powder and 10 g/kg garlic powder+2 g/kg black pepper powder significantly improved the weight gain and FCR.

  6. Replacement Value of Untreated or Fungal Treated Carrot Leaves for Corn in Broiler Diet

    International Nuclear Information System (INIS)

    El-Faramawy, A.A.

    2006-01-01

    Three hundred (21 days old) Arbor Acre chicks were used to evaluate the replacement value of untreated or Aspergillus niger treated carrot leaves for corn in broiler diets. Birds were fed a control diet or diets in which 10% untreated or treated carrot leaves was quantitatively substituted for corn in the control diet. Replacement of yellow corn with 10% untreated and treated carrot leaves caused insignificant (P 0.01) in both experimental groups compared to control. These results denoted that although there were negligible changes in body weight and mortality rate in broiler chicken fed untreated and treated carrot leaves the amino acid profile of carcasses lessen their nutritive value which is in consequence reflected negatively on human amino acids intake. Poultry production represents one of the quickest means of correcting the anomaly of protein inadequacy, yet the rising cost of feed which represents 70-80% of the cost of production among other costs, is a major setback (Opera. 1996). The price of most conventional feed ingredients such as yellow corn, soybean meal and fish meal is so high in recent time that it is becoming uneconomical to use them in poultry feeding (Esonu et al., 2001). Consequently poultry feed researchers have been forced to seek alternative and cheaper feed resources

  7. Serum cation profile of broilers at various stages of exposure to deoxynivalenol.

    Science.gov (United States)

    Yunus, Agha Waqar; Böhm, Josef

    2013-05-01

    The present experiment was carried out to investigate if levels of serum cations in broilers are modulated differently at various stages of exposure to deoxynivalenol (DON). Male broiler chicks at 7 days of age were fed a basal diet (0.27 mg of DON; 0.01 mg of zearalenone/kg), or either a low DON diet (1.68 mg of DON; 0.15 mg of zearalenone/kg) or a high DON diet (12.21 mg of DON; 1.09 mg of zearalenone/kg) produced using extracts from Fusarium graminearum cultures. Blood samples from the birds were collected during weeks 2, 4, and 5 of exposure. The high DON diet resulted in lower serum calcium levels compared to the basal diet at all the 3 sampling stages, while the low DON diet resulted in lower serum calcium levels only during weeks 2 and 5. Serum potassium levels were reduced under both the DON diets during weeks 2 and 5, while no diet-associated changes were found for serum levels of magnesium, sodium, and zinc. Under the present experimental conditions, the serum levels of calcium were consistently modulated in the broilers exposed to the DON-contaminated diets. The modulation of serum levels of potassium was, however, dependent upon the stage of exposure to DON.

  8. Dietary vitamin E (α-tocopheryl acetate) and organic selenium ...

    African Journals Online (AJOL)

    enriched yeast) and vitamin E (α-tocopheryl acetate) supplementation, alone or together, on the performance and antioxidant status of broilers fed diets enriched in n-3 PUFAs using fish oil. Day-old Hubbard-JV strain broiler chicks allocated to one ...

  9. Intestinal Development and Function of Broiler Chickens on Diets Supplemented with Clinoptilolite

    Directory of Open Access Journals (Sweden)

    Q. J. Wu

    2013-07-01

    Full Text Available The purpose of this study was to evaluate the effect of natural clinoptilolite (NCLI and modified clinoptilolite (MCLI on broiler performance, gut morphology, intestinal length and weight, and gut digestive enzyme activity. A total of 240 d-old male chicks were randomly assigned to 3 treatments, each of which comprised 8 pens of 10 chicks per pen. Birds in the control group were fed the basal diet, while those in the experimental groups were fed diets supplemented with NCLI at 2% (NCLI group, or MCLI at 2% (MCLI group, respectively, for 42 d. Compared with the control, supplementation with NCLI or MCLI had no significant (p>0.05 effects on productive parameters from d 1 to 42. Supplementation with NCLI or MCLI had no influence on the relative length and weight of small intestine at d 1 to 21. But supplementation with NCLI or MCLI significantly reduced the relative weight of duodenum. Supplementation with MCLI and NCLI was associated with greater (p0.05 influence on the crypt depth in the jejunal and ileal mucosa compared with those in the controls. The addition of either NCLI or MCLI to the diet improved the activities of total protease, and amylase in the small intestinal contents. In conclusion, supplementation with NCLI or MCLI in diets improved intestinal morphology, increased the intestinal length and weigh and gut digestive enzyme activity.

  10. Performance of broilers fed enzyme-supplemented tigernut ...

    African Journals Online (AJOL)

    A feeding trial was set up to study the effects of replacing maize with tigernut meal (TGN) at 0, 33.33, 66.67 and 100 per cent levels, with 0.10 per cent enzyme supplementation of all levels, on performance characteristics and carcass yield in broiler chicken for 8 weeks (56 days). A total of 200 Anak-2000 breed of broilers ...

  11. Decay of maternal antibodies in broiler chickens.

    Science.gov (United States)

    Gharaibeh, Saad; Mahmoud, Kamel

    2013-09-01

    The objective of this study was to determine the decay rate of maternal antibodies against major broiler chicken pathogens. A total of 30 one-day-old broiler chicks were obtained from a commercial hatchery and reared in isolation. These chicks were retrieved from a parent flock that received a routine vaccination program. Chicks were bled at hatch and sequentially thereafter every 5 d through 30 d of age. Maternal antibody titers were measured by ELISA for avian encephalomyelitis (AEV), avian influenza virus (AIV), chicken anemia virus (CAV), infectious bursal disease virus (IBDV), infectious bronchitis virus (IBV), infectious laryngotracheitis virus (ILTV), Mycoplasma gallisepticum (MG), Mycoplasma synoviae (MS), and reovirus (Reo). Maternal antibody titers for Newcastle disease virus (NDV) were measured using a hemagglutination inhibition test. Half-life estimates of maternal antibody titers were 5.3, 4.2, 7, 5.1, 3.9, 3.8, 4.9, 4.1, 6.3, and 4.7 d for AEV, AIV, CAV, IBDV, IBV, ILTV, MG, MS, NDV, and Reo, respectively. The statistical analysis revealed significant differences among half-lives of maternal antibody titers against certain pathogens. Furthermore, all maternal antibody titers were depleted by 10 d of age except for IBDV.

  12. Effects of dietary on antioxidant status, lipid profile, immune response and performance characteristics of broiler chickens reared under high ambient temperature

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    Sara Mirzaie

    2018-04-01

    Full Text Available Objective Spirulina has been recognized formerly as a filamentous spiral-shaped blue-green algae but more recently as a genus of photosynthetic bacteria (Arthrospira. This microorganism is considered as a rich source of essential nutrients for human and animals. The present study was conducted to determine potential application of Spirulina for heat-exposed broilers. Methods Two hundred and fifty Cobb 500 chicks with male to female in equal ratio with average initial weight of 615.6 g at 17 days of age were divided into 5 treatments with 5 replicates of 10 chicks. Treatment groups were as follows: positive and negative controls with 0% Spirulina supplement and three Spirulina receiving groups with 5 g/kg (0.5%, 10 g/kg (1%, and 20 g/kg (2% supplementation. Spirulina receiving groups as well as positive control were exposed to high ambient temperature at 36°C for 6 h/d from 38 to 44 days of age. Biochemical variables were measured in serum samples at 35, 38, 42, and 45 days of broiler chickens age. Results The results showed that supplementation of the diet with Spirulina decreased concentration of stress hormone and some serum lipid parameters while enhanced humoral immunity response and elevated antioxidant status whereas it didn’t meaningfully affect performance characteristics. Nevertheless, feed conversion ratio was improved numerically but not statistically in broilers fed with 1% Spirulina under high ambient temperature. Conclusion Overall, the present study suggests that alleviation of adverse impacts due to high ambient temperature at biochemical level including impaired enzymatic antioxidant system, elevated stress hormone and lipid profile can be approached in broiler chickens through supplementation of the diet with Spirulina platensis.

  13. Efisiensi Penggunaan Energi Metabolis Ransum Berbasis Onggok yang Difermentasi Bacillus amyloliquefaciens pada Ayam Broiler

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    Wizna

    2008-12-01

    Full Text Available An experiment was conducted to determine the effects of using Bacillus amyloliquefaciens-fermented tapioca by-products (onggok on the metabolism energy differences in the diets to the performance of broilers. Eighty unsexed three day old broiler chicks were randomly allocated into 20 pens (four chicks/pen. This experiment was arranged in a completely randomized design with four dietary treatments (3000, 2900, 2800 and 2700 kkal/kg for broilers of energy metabolism in diets and five replications. Measured variables were feed consumption, average body weight gain, feed conversion, carcass percentage, abdominal fat percentage and income over feed cost (IOFC. Results of the experiment indicated that feed consumption, average body weight gain, feed conversion, carcass percentage, abdominal fat percentage and income over feed cost (IOFC were not affected (P>0.05 by levels of metabolism energy in the diets for broilers. In conclusion, Bacillus amyloliquefaciens-fermented tapioca by-products (onggok can be included up to 40% with energy metabolism 2700 kkal/kg in broilers diets and improved the efficiency of metabolizable energy.

  14. Comparative evaluation of different soybean meal and the replacement using peanut, rapeseed and fish meal for broilers .

    Directory of Open Access Journals (Sweden)

    Budi Tangendjaja

    1996-06-01

    Full Text Available Two feeding experiments have been conducted to test the different sources of soybean meal (local, India, USA and the partial substitution with peanut meal (10%, rapeseed meal (5% and fish meal (5% in the diet for broiler. The first feeding experiment was carried out on broiler starter for 4 weeks in wire cages . Twelve rations in factorial design (3 x 4 were conducted using 6 birds/cage and replicated 6 times . Birds fed India soybean meal had significantly less body weight (1,000 g than those fed local or USA soybean meals (1,037 and 1,023 g, respectively . Partial substitution with peanut, rapeseed or fish meal did not affect body weight, feed consumption or feed/gain ratio . The average consumption and feed/gain were 1,535 g and 1,569, respectively . In second experiment, one thousand five hundred broiler chicks were allocated in 6 dietary treatments in factorial design (3 x 2 . Factor one was different sources of soybean meal (local, India and USA and factor two was the inclusion of peanut meal at 0 and 10%. Birds were kept on litter system for 6 weeks . Each treatment used 5 replicates and 50 birds/replicate . The results show no effect of the treatment to any parameter measured . Body weight of bird fed local, India and USA soybean meal were 1,662, 1,641 and 1,669 g and feed/gain were 2 .043, 2 .051 and 2.035, respectively . Cost analysis indicates that ration uses local soybean meal gives a higher cost than those of India and USA soybean meals.

  15. Small intestinum morphology of broiler chickens on diets ...

    African Journals Online (AJOL)

    Zikic

    2011-07-04

    Jul 4, 2011 ... Influence of dietary mannanoligosaccharides on histological ... The trial involved 480 Hubbard Classic broiler chicks which were from either .... Stocking density was 16 ... ratio was also noticed at day 28 in BM/BM group as.

  16. Evaluation of a Bacillus direct-fed microbial candidate on digesta viscosity, bacterial translocation, microbiota composition and bone mineralisation in broiler chickens fed on a rye-based diet.

    Science.gov (United States)

    Latorre, J D; Hernandez-Velasco, X; Bielke, L R; Vicente, J L; Wolfenden, R; Menconi, A; Hargis, B M; Tellez, G

    2015-01-01

    1. The effects of the dietary inclusion of a Bacillus-based direct-fed microbial (DFM) candidate on digesta viscosity, bacterial translocation, microbiota composition and bone mineralisation were evaluated in broilers consuming rye-based diets. 2. In the present study, control mash rye-based diets (CON) or Bacillus-DFM supplemented diets (TRT) were administered ad libitum to male broilers in three independent experiments. 3. In Experiments 1 and 2 (n = 25/group), liver samples were taken to evaluate bacterial translocation, digesta samples were used for viscosity measurements and the intestinal microbial flora was evaluated from different intestinal sections to enumerate total recovered gram-negative bacteria (TGB), lactic acid bacteria (LAB) and anaerobic bacteria (TAB). Additionally, both tibias were removed for assessment of bone quality. 4. In Experiment 3, each experimental group had 8 replicates of 20 chickens (n = 160/group). Weekly, body weight (BW), feed intake (FI) and feed conversion ratio (FCR) were evaluated. At d 28-of-age, samples were taken to determine bacterial translocation, digesta viscosity and bone quality characteristics. 5. In all experiments, consumption of Bacillus-DFM reduced bacterial translocation to the liver and digesta viscosity. Additionally, DFM supplementation improved BW, bone quality measurements and FCR. Moreover, chickens fed on the Bacillus-DFM diet in Experiments 1 and 2 showed a significant reduction in the number of gram-negative and anaerobic bacteria in the duodenal content compared to control. 6. In summary, chickens fed on a rye-based diet without DFM inclusion showed an increase in bacterial translocation and digesta viscosity, accompanied by reduced performance and bone quality variables relative to the Bacillus-DFM candidate group. Hence, incorporation into the feed of a selected DFM ameliorated the adverse anti-nutritional effects related to utilisation of rye-based diets in broilers chickens.

  17. Desempenho, eficiência de utilização dos nutrientes e estrutura do trato digestório de pintos de corte alimentados na fase pré-inicial com rações de diferentes formas físicas Performance, efficiency of nutrient utilization and gastrointestinal structures of broiler chick fed in prestarter phase with ratios with different physical form

    Directory of Open Access Journals (Sweden)

    Ednardo Rodrigues Freitas

    2008-01-01

    Full Text Available Este estudo foi realizado com o objetivo de avaliar o efeito da forma física da ração sobre o desempenho, a utilização dos nutrientes das rações e o desenvolvimento do trato disgestório de pintos de corte na primeira semana de vida. Foram utilizados 150 pintos machos Cobb distribuídos ao acaso em três tratamentos com cinco repetições de dez aves. Os tratamentos consistiram das formas físicas farelada, triturada e peletizada de uma ração pré-inicial comercial. Com exceção da moela, os órgãos do trato digestório e a estrutura morfométrica do intestino não foram influenciados pela forma física da ração. Maior digestibilidade do N foi obtida com a ração peletizada, enquanto a ração triturada possibilitou maior digestibilidade do EE. A energia metabolizável determinada para a ração farelada foi menor que a obtida nas demais formas físicas da ração. As aves alimentadas com raç��o farelada tiveram menor consumo de ração e ganho de peso e pior conversão alimentar. Ingeriram ainda menos energia, apresentaram menores retenções de proteína, gordura e energia corporal e utilizaram maior proporção da energia ingerida para produção de calor. As rações pré-iniciais na forma peletizada ou triturada possibilitam maior aproveitamento dos nutrientes da ração pelos pintos e melhor desempenho.This experiment was conducted to evaluate the effect of different physical form of pre-starter diet on performance, nutrient utilization and development of gastrointestinal tract of broiler chicks during the first week of age. A total of 150-day-old male broiler chicks was allotted to a completely randomized design with three treatments and five replicates of 10 birds each. Treatments consisted of three physical forms of a commercial pre-starter diet: mashed, crumbled and pressed into pellets. Except for gizzard, the other organs of the digestive tract and morphologic structures of the small intestine were not affected by

  18. Influence of non starch polysaccharide-degrading enzymes on the ...

    African Journals Online (AJOL)

    enzymes on the performance, meat yield, water intake, litter moisture and jejunal digesta viscosity of chicks fed wheat/barley based diet. A total of 195 1-d-old male broiler chicks (Ross 308) were allocated to 5 treatment groups, with 3 replicates per ...

  19. Studies on the feeding of cupric sulfate pentahydrate, cupric citrate, and copper oxychloride to broiler chickens.

    Science.gov (United States)

    Ewing, H P; Pesti, G M; Bakalli, R I; Menten, J F

    1998-03-01

    Male commercial broiler strain chickens were fed either a control diet (based on corn and soybean meal) or the control diet supplemented with cupric sulfate pentahydrate, copper oxychloride, or cupric citrate in two experiments conducted in floor pens. In Experiment 1, feeding copper at 125 mg/kg diet for 42 d significantly increased broiler growth; and the response from cupric citrate was significantly better than either cupric sulfate or copper oxychloride. In Experiment 2, the inclusion of copper from cupric citrate was reduced to 63 mg/kg and the length of the experiment was increased to 56 d. Cupric sulfate pentahydrate and copper oxychloride treatments increased weight gain by 4.9% and cupric citrate increased weight gain by 9.1%. The feed conversion ratios (grams of feed:grams of gain of live birds) in the birds fed copper were not significantly different from those fed the basal diet (P > 0.05) unless corrections were made for the weights of the dead birds; the adjusted feed conversion ratios (grams of feed:grams of gain of live birds + grams of gain of mortalities) for the copper-treated birds in Experiments 1 and 2 were 5.2 and 7.6% lower, respectively, than the ratios of birds fed the basal diets. Plasma copper levels increased in supplemented chicks by 35% in Experiment 1 and 24% in Experiment 2. Liver copper levels in both experiments were increased by 26% with copper supplementation. Mortality was not affected by dietary treatment in either experiment (P > 0.05).

  20. Exploratory transcriptomic analysis in muscle tissue of broilers fed a phytase-supplemented diet.

    Science.gov (United States)

    Schmeisser, J; Séon, A-A; Aureli, R; Friedel, A; Guggenbuhl, P; Duval, S; Cowieson, A J; Fru-Nji, F

    2017-06-01

    The effect of phytase on phosphorus retention, broiler (Gallus gallus) performance and bone mineralization in diets with reduced inorganic phosphate concentration is well documented. Furthermore, so-called 'extra-phosphoric' effects of phytase have been described in the literature that may be associated with changes in mineral and amino acid partitioning and requirements per se. In particular, the role of myo-inositol in phytase responses is implied but not well elucidated. It was the purpose of the experiment reported herein to explore the effect of phytase on broiler growth, nutrient digestibility, blood biochemistry and gene expression. A 5-week broiler floor pen trial was conducted to evaluate the effect of supplementation of a moderately phosphorus-deficient diet with 1000 U/kg of a 6-microbial phytase. Parameters measured were growth performance, phosphorus (P), calcium (Ca) and myo-inositol plasma concentrations, apparent ileal P digestibility, bone mineralization, breast meat weight and Pectoralis major muscle transcriptome. Supplementation of the diet with phytase improved weight gain during the starter period (18%) and the whole period (24%) compared with animals that received the control diet (p phytase. The transcriptomic analysis revealed that some differentially expressed genes (DEG) in broilers, receiving phytase in comparison with animals fed reduced phosphorus diet without phytase, were part of pathways involved in muscle development, via calmodulin/calcineurin and insulin-like growth factor. Microarray data confirmation was performed on six genes by quantitative PCR (qPCR): PI3K regulatory and catalytic subunit, Phospholipase C beta, Myocyte Enhancer Factors 2A and 2C, and calcineurin A. The results suggested that dietary supplementation with this phytase could generate low molecular weight phytate esters and indirectly myo-inositol, and could help us to understand how muscle metabolism may be affected at a gene level. Journal of Animal

  1. Increased iron level in phytase-supplemented diets reduces performance and nutrient utilisation in broiler chickens.

    Science.gov (United States)

    Akter, Marjina; Iji, P A; Graham, H

    2017-08-01

    1. The effect of different levels of dietary iron on phytase activity and its subsequent effect on broiler performance were investigated in a 3 × 2 factorial arrangement. A total of 360 day-old Ross 308 male broiler chicks were distributed to 6 experimental diets, formulated with three levels of Fe (60, 80 and 100 mg/kg) and two levels of phytase (0 and 500 FTU/kg). 2. Phytase supplemented to mid-Fe diets increased feed consumption more than the non-supplemented diet at d 24. From hatch to d 35, Fe × phytase interaction significantly influenced the feed intake (FI), body weight gain (BWG) and feed conversion ratio (FCR). The high-Fe diet supplemented with phytase significantly reduced FI and BWG of broilers than those supplemented with low- or mid-Fe diets. The overall FCR was significantly better in birds fed on the mid-Fe diets with phytase supplementation. 3. A significant improvement in ileal digestibility of N, P, Mg and Fe was observed in birds feed diets containing 60 mg Fe/kg, with significant interaction between Fe and phytase. 4. Phytase improved the bone breaking strength when supplemented to low- or mid-Fe diets, compared to the non-supplemented diets. There was a significant Fe × phytase interaction effect. Tibia Fe content was higher in birds fed on phytase-free diets with high Fe but the reverse was the case when phytase was added and their interaction was significant. High dietary Fe significantly increased the accumulation of Fe in liver. 5. Phytase improved Ca-Mg-ATPase, Ca-ATPase and Mg-ATPase activities in jejunum when supplemented to the diet containing 80 mg Fe/kg. 6. This study indicates that high (100 mg/kg) dietary Fe inhibited phytase efficacy and subsequently reduced the overall performance and nutrient utilisation of broilers.

  2. Intestinal mucosa development in broiler chickens fed natural growth promoters

    Directory of Open Access Journals (Sweden)

    ERL Pelicano

    2005-12-01

    Full Text Available This study evaluated the use of probiotics and prebiotics on the histological and morphological indexes of the intestinal mucosa of broilers at 21 days of age. Thirty-six birds were randomly distributed in a 3 x 3 factorial arrangement, considering 3 probiotics and prebiotics sources in the diet. There were 9 treatments with 4 repetitions. Diet treatments were: 1 - Control (without growth promoters; 2 - Bacillus subtilis-based probiotic (Pro 1; 3 - Probiotic (Pool based on Lactobacillus acidophilus and casei, Streptococcus lactis and faecium, Bifidobacterium bifidum and Aspergillus oryzae (Pro 2; 4 - Prebiotic based on Phosphorylated Mannanoligosaccharide (MOS and Organic Acidifier (OA (Pre 1; 5 - MOS-based prebiotic (Pre 2; 6 - Pro 1 + Pre 1; 7 - Pro 1 + Pre 2; 8 - Pro 2 + Pre 1; 9 - Pro 2 + Pre 2. Higher villus height (VH (p<0.01 were seen in the duodenum of birds fed diets without prebiotics, whereas birds fed Bacillus subtilis-based probiotic and birds fed prebiotic based on MOS and OA showed higher VH (p<0.01 in jejunum and ileum. Greater crypt depths (CD (p<0.01 were observed in the duodenum, jejunum and ileum of birds receiving B. subtilis, and in the duodenum and jejunum of birds fed diets without prebiotics. Significant interaction (p<0.01 between the evaluated factors was seen for both, VH and CD, in the three intestinal portions. Greater VH was obtained in duodenum, jejunum and ileum with the use of probiotics and prebiotics and greater CD with the use of probiotics, in relation to the control group. There was no difference in villus density (VD between birds fed diets without additives or diets containing probiotics and prebiotics. Nevertheless, there was a significant interaction (p<0.05 between the evaluated factors for VD in the duodenum. Concluding, beneficial effects were seen in histological indexes of the intestinal mucosa with the use of probiotics and prebiotics at 21 days of age.

  3. Effect of garlic and neem leaf powder supplementation on growth performance and carcass traits in broilers

    Directory of Open Access Journals (Sweden)

    Karan Rajendra Kharde

    2014-10-01

    Full Text Available Aim: Present experiment was designed to study the effect of supplementation of garlic and neem leaf powder (NLP on growth performance and carcass yields in broilers. Materials and Methods: A total of 210-day-old commercial male broiler chicks (Ven Cobb were procured and randomly distributed in to seven treatments, each treatment had six replicates with five chicks per each replicate. Growth trial was conducted in a randomized block design comprising seven dietary treatments. T1 was served as control. T2 and T3 were fed with garlic powder (GP 0.5, 1 g/kg feed respectively. T4 and T5 were given with NLP 1, 2 g/kg feed respectively. T6 was fed with 0.5 g/kg GP and 1.0 g/kg NLP. T7 was treated with 1.0 g/kg GP and 2.0 g/kg NLP. The body weight, feed consumption of individual bird was recorded at weekly interval and the body weight gain, feed conversion ratio (FCR were calculated. At the end of 42 day, six birds from each dietary group were sacrificed to estimate the dressing yield and relative weights of giblet (liver, heart and gizzard. Results: Supplementation of garlic and NLP alone and their combination significantly (p0.05 influence was observed in carcass parameters like dressing yield and giblet yield (heart, gizzard and liver in all treatment groups. Conclusions: Supplementation of GP alone showed poor performance, but when it was supplemented in combination with NLP there was improvement in performance parameters. These results indicated that the combination of GP and NLP can be used as alternates to coccidiostats for improving the performance. Such meat can be used as a designer meat as fetch higher price if marketed as branded item.

  4. Effects of rye inclusion in broilers

    NARCIS (Netherlands)

    Schokker, D.; Krimpen, van M.M.; Vastenhouw, S.A.

    2017-01-01

    An experiment was conducted to investigate the effects of dietary inclusion of rye, a model ingredient to increase gut viscosity, between 14 and 28 days of age on immune competence related parameters and performance of broiler. A total number of 960 one-day-old male Ross 308 chicks were weighed and

  5. Hepatic glutathione and glutathione S-transferase in selenium deficiency and toxicity in the chick

    International Nuclear Information System (INIS)

    Kim, Y. S.

    1989-01-01

    First, the hepatic activity of GSH-T CDNB was increased only under conditions of severe oxidative stress produced by combined Se- and vitamin E (VE)-deficiency, indicating that VE also affects GSH metabolism. Second, the incorporation of 35 S-methionine into GSH and protein was about 4- and 2-fold higher, respectively, in Se- and VE-deficient chick hepatocytes as compared to controls. Third, chicks injected with the glutathione peroxidase (SeGSHpx) inhibitor, aurothioglucose (AuTG), showed increase hepatic GSH-T CDNB activity and plasma GSH concentration regardless of their Se status. Fourth, the effect of ascorbic acid (AA), on GSH metabolism was studied. Chicks fed 1000 ppm AA showed decreased hepatic GSH concentration compared to chicks fed no AA in a Se- and VE-deficient diet. Fifth, chicks fed excess Se showed increase hepatic activity of GSH-T CDNB and GSH concentration regardless of VE status

  6. Ojediran, TK

    African Journals Online (AJOL)

    Ojediran, TK. Vol 9, No 1-2 (2013) - Articles Biochemical and Heamatological Indices of Broiler Chickens fed Differently Processed Legume Seed Meals Abstract PDF · Vol 9, No 1-2 (2013) - Articles Lesions in Broiler Chicks Following Experimental Contamination with Battery Waste Abstract PDF. ISSN: 0331-5428.

  7. Effect of Litter Moisture on the Development of Footpad Dermatitis in Broiler Chickens

    OpenAIRE

    TAIRA, Kazuyo; NAGAI, Toshimune; OBI, Takeshi; TAKASE, Kozo

    2013-01-01

    ABSTRACT Broiler chicks were reared on either wet litter or dry litter to compare the development of footpad dermatitis (FPD). Broilers reared on wet litter first developed FPD at 14 days of age. Their FPD scores increased sharply after 21 days of age, reaching 2.92 at 42 days. In broilers reared on dry litter, FPD was first observed at 28 days of age, and the FPD score was only 0.70 at 42 days. When 21- or 28-day-old broilers that had been reared on wet litter and had developed FPD were move...

  8. Growth performance of broilers in experimental Reovirus infections

    Directory of Open Access Journals (Sweden)

    Sudhakar P. Awandkar

    Full Text Available Background: The avian reoviruses have emerged to induce various manifestations in chickens. They are associated with disease conditions including malabsorption syndrome, tenosynovitis etc. Reoviruses are an important cause of suboptimum performance in broilers, resulting in poor growth performance. Poultry industry in India is facing a catastrophe due to such infections which go unnoticed in field due to masking of the symptoms by secondary infections and commonly observed nutritional disorders. Aim: To investigate the effect of reovirus infection on overall performance of broiler birds. Material and Methods: The broiler birds were challenged with homologous strains of malabsorption syndrome and tenosynovitis syndrome of reovirus. The growth performance was recorded. Results and conclusion: The growth performance and immune response to NDV did not differ in the birds challenged with tenosynovitis syndrome strain of reo virus as compared to un challenged birds. However, poor live body weight, feed intake, FCR, PE and BPEI and better serum NDV titres were found in chicks challenged with malabsorption syndrome strain of reovirus as compared to the chicks from control group. [Vet World 2012; 5(11.000: 685-689

  9. Effect of ethanolic extract of propolis as an alternative to antibiotics as a growth promoter on broiler performance, serum biochemistry, and immune responses

    Directory of Open Access Journals (Sweden)

    Abbasali Gheisari

    2017-02-01

    Full Text Available Aim: An in vivo experiment was conducted to investigate the effect of different levels of ethanolic extract of propolis, on growth performance, carcass traits, serum biochemistry, and humoral immune responses of chickens, as compared with the antibiotic flavophospholipol. Materials and Methods: 312 1-day-old as-hatched broiler chicks (Ross 308 were randomly allotted to 6 treatments with 4 replicate pens per treatment. The 6 dietary treatments fed for 42 days consisted of a corn-soybean meal basal diet (control; control plus 4.5 mg/kg flavophospholipol, and control plus 50, 100, 200, and 300 mg/kg ethanol extracts of propolis, respectively. Results: Neither propolis nor antibiotic affected the performance criteria; however, dietary treatments tended to enhance to enhance body weight and daily feed intake of broiler chickens compared with control group (p>0.05. None of the dietary treatments significantly altered feed: Gain though; broilers fed diet supplemented with 200 mg/kg propolis had better feed: gain values compared with other groups in starter, and grower phases as well as the whole experimental period (p>0.05. Carcass yield and internal organ relative weights were not affected by treatments on day 42, except for abdominal fat pad weight that decreased in broilers supplemented with antibiotic. None of the treatments significantly affected humoral immune function. Dietary treatments failed to induce any significant effect on serum biochemistry (p>0.05; though broilers receiving 100 mg/kg propolis had greater high-density lipoprotein-cholesterol and lower triglyceride concentrations compared with other groups. Conclusion: In conclusion, the results indicated that addition of ethanolic extract of propolis to routine dietary components of broilers, such as corn and soybean, seems not to have a positive influence on performance criteria.

  10. Comparative study on immuno-modulatory effect of anticoccidial vaccines and a coccidiostat on nd vaccinated broiler chicks

    International Nuclear Information System (INIS)

    Hazeen, M.K.; Mustafa, M.Y.; But, T.M.

    2007-01-01

    105 day old broiler chicks were divided into 5 equal groups with 21 chicks in each i.e. A (non vaccinated, non medicated control), B (ND vaccinated control), C (administered with ND vaccine Lasota and Eimeria vaccine EV), D (NDV and immucox) and E (administered with NDV vaccine and coccidiostat). The comparative immunomodulatory effects of EV, immucox and Sacox were worked out on the basis of the GMT levels in Newcastle disease vaccinated birds along with the non-vaccinated and non-medicated control birds. These titres were evaluated by using HA and H1 tests on the sera of these experimental chicks. Other parameters i.e. morbidity, mortality, OPG, weight gain, FCR, Postmortem findings, Bursal/body weight ratio, weight, size and texture of spleen, thymus and liver were also assessed. The birds that were kept as NDV vaccinated control (group B) had the highest body weight and showed best FCR. The birds of unmedicated, unvaccinated control group (A) secured 2nd position regarding weight gain and FCR. Among the three experimental groups (C, D and E), the birds from group C (NDV + EV) had higher body weight than group D and E. Feed conversion ratio (FCR) of the birds of group C was also found to be better than the birds belonging to group D and E. The number of oocysts per gm of faeces showed gradual decrease and became nil on the observation of day 33 of the experiment, as the birds became solidly immune against Eimeria infection at this age. The birds belonging to group B (NDV vaccinated control) had shown the highest antibody titers while the birds of group A (unvaccinated unmedicated control) had the lowest antibody titres. Among the three experimental groups (C, D and E) the birds from group C had the higher antibody titres as compared to other two groups (D and E) on day 49. (author)

  11. Qualidade da carne de frangos de corte submetidos a dietas suplementadas com crômio, criados em diferentes temperaturas ambientais = Meat quality of broiler chicks submeted the supplemented diets with chromium and reared in differents enviromental temperatures

    Directory of Open Access Journals (Sweden)

    Alexandre Oba

    2007-04-01

    Full Text Available Foram realizados dois experimentos. O experimento um objetivou avaliar a composição da carne do peito de frangos alimentados com dietas suplementadas com crômio (0, 400, 800 e 1.200 μg Cr kg-1 complexado a levedura, criados sob diferentes condições de temperatura (quente, termoneutra e fria. O experimento dois objetivou avaliar a qualidade da carne do peito de frangos alimentados com dietas suplementadas com crômio (0 e 400 μg Cr kg-1, criados nas mesmas condições do experimento um e desossados em diferentes períodos (0, 5 e 24 horas. Os resultados mostram que as aves criadas em ambiente frio apresentaram a carne com menor porcentagem de matéria mineral e crômio e maior intensidade de vermelho, enquanto as aves criadas em temperatura termoneutra apresentaram carne com menor porcentagem de umidade, e as aves criadas em temperatura quente, carne com maior luminosidade. Já a suplementação de crômio não proporcionou qualquer efeito na composição e na qualidade de carne, enquanto que o aumento no período de desossa proporcionou um menor comprimento de sarcômero, força de cisalhamento e intensidade de amarelo, porém uma maior luminosidade.Two experiments were conducted being the objective of the first experiment to evaluate the the composition of breast meat of broiler chicks fed with diets supplemented with chromium yeast (0; 400; 800and 1,200 μg Cr kg-1 reared in different environmental temperatures (hot, thermoneutral and cold. The second experiment aimed to evaluate the quality of breast meat of broiler chicks fed with diets supplemented with chromium yeast (0 and 400 μg Cr kg-1, reared in different environmental temperatures (hot, thermoneutral and cold and deboned in different periods (0, 5 and 24 hours. The results showed that birds reared in cold environmental temperature showed meat with lower mineral matter percentage and chromium and higher values of redness, whereas the birds reared in thermoneutral environment

  12. Use of Vitamin D and Its Metabolites in Broiler Chicken Feed on Performance, Bone Parameters and Meat Quality

    Directory of Open Access Journals (Sweden)

    Ana Flávia Quiles Marques Garcia

    2013-03-01

    Full Text Available The objective of this experiment was to assess the use of different vitamin D metabolites in the feed of broiler chickens and the effects of the metabolites on performance, bone parameters and meat quality. A total of 952 one-day-old male broiler chicks were distributed in a completely randomised design, with four treatments, seven replicates and 34 birds per experimental unit. The treatments consisted of four different sources of vitamin D included in the diet, D3, 25(OHD3, 1,25(OH2D3, and 1α(OHD3, providing 2000 and 1600 IU of vitamin D in the starter (1 to 21 d and growth phases (22 to 42 d, respectively. Mean weight, feed:gain and weight gain throughout the rearing period were less in animals fed 1α(OHD3 when compared with the other treatments (p0.05 for various bone parameters. Meat colour differed among the treatments (p>0.05. All of the metabolites used in the diets, with the exception of 1α(OHD3, can be used for broiler chickens without problems for performance and bone quality, however, some aspects of meat quality were affected.

  13. Efficacy of New 6-Phytase from spp. on Growth Performance and Nutrient Retention in Broiler Chickens Fed Corn Soybean Meal-based Diets

    Directory of Open Access Journals (Sweden)

    E. Kiarie

    2015-10-01

    Full Text Available A total of 420 day-old male Ross chicks were weighed at d 1 of life and assigned to test diets to assess the efficacy of a new Buttiauxella spp. phytase expressed in Trichoderma reesei. Diets were: positive control (PC adequate in nutrients and negative control (NC diet (40% and 17% less available phosphorous (P and calcium (Ca, respectively supplemented with 6 levels of phytase 0, 250, 500, 750, 1,000, and 2,000 phytase units (FTU/kg of diet. All diets had titanium dioxide as digestibility marker and each diet was allocated to ten cages (6 birds/cage. Diets were fed for 3 wk to measure growth performance, apparent retention (AR on d 17 to 21 and bone ash and ileal digestibility (AID on d 22. Growth performance and nutrient utilization was lower (p<0.05 for NC vs PC birds. Phytase response in NC birds was linear (p<0.05 with 2,000 FTU showing the greatest improvement on body weight gain (20%, feed conversion (7.4%, tibia ash (18%, AR of Ca (38%, AR of P (51% and apparent metabolizable energy corrected for nitrogen (5.1% relative to NC. Furthermore, phytase at ≥750 FTU resulted in AID of total AA commensurate to that of PC fed birds and at ≥1,000 FTU improved (p<0.05 AR of P, dry matter, and N beyond that of the lower doses of phytase and PC diet. In conclusion, the result from this study showed that in addition to increased P and Ca utilization, the new Buttiauxella phytase enhanced growth performance and utilization of other nutrients in broiler chickens in a dose-dependent manner.

  14. Efficacy of beer fermentation residue containing Saccharomyces cerevisiae cells for ameliorating aflatoxicosis in broilers.

    Science.gov (United States)

    Bovo, F; Franco, L T; Kobashigawa, E; Rottinghaus, G E; Ledoux, D R; Oliveira, C A F

    2015-05-01

    This study aimed to determine the aflatoxin B1 (AFB1) binding capacity of a beer fermentation residue (BFR) containing Saccharomyces cerevisiae cells, and the efficacy of BFR to ameliorate the toxic effects of AFB1 on performance, serum biochemistry, and histology of broilers. The BFR was collected from a microbrewery, and the yeast cells were counted, dried, and milled before it was used in the study. In vitro evaluation of the BFR was conducted using different concentrations of AFB1 (2.0, 4.0, 8.0, 16.0, and 32.0 μg AFB1/mL) and 100 mg/10 mL of BFR at pH 3.0 or 6.0. Two hundred 1-day-old male broilers (Ross 308) were assigned to chick batteries and allowed ad libitum access to feed and water. A completely randomized design was used with 5 replicate pens of 5 chicks assigned to each of 4 dietary treatments from hatch to 21 d, which included: 1) basal diet (BD), with no BFR or AFB1; 2) BD supplemented with 1% BFR; 3) BD supplemented with 2 mg AFB1/kg of feed; and 4) BD supplemented with 2 mg AFB1/kg feed and 1% BFR. Performance variables were determined weekly, while serum analyses were performed on d 14 and 21. At the end of the study, chicks were anesthetized with carbon dioxide, euthanized by cervical dislocation, and the kidney, liver, and bursa of Fabricius were removed for determination of relative weights, and for histological evaluation. In vitro assays showed that the higher the initial AFB1 concentration in solution, the greater the AFB1 amount adsorbed by BFR at both pHs tested. Feed intake, BW gain, and concentrations of albumin, total protein, and globulin increased (P < 0.05) in broilers fed BFR+AFB1 (Diet 4), when compared to the birds receiving only AFB1 (Diet 2). Although BFR was not able to reduce or prevent the effects of AFB1 on relative weights of kidneys and liver, it reduced the severity of histological changes in the liver and kidney caused by AFB1. © 2015 Poultry Science Association Inc.

  15. Mineral profiling, carcass quality and sensory evaluation of broiler ...

    African Journals Online (AJOL)

    An investigation to determine the mineral composition, carcass quality and organoleptic properties of the meat of broilers fed basal broiler feeds supplemented with different leaf meals was conducted. Four treatments: basal proprietary broiler feed only (T1 - PBF) as control, basal proprietary broiler feeds supplemented with ...

  16. EFFECT OF TURMERIC (CURCUMA LONGA SUPPLEMENTATION ON GROWTH AND BLOOD CHEMISTRY OF BROILERS

    Directory of Open Access Journals (Sweden)

    Kamdev Sethy

    2016-06-01

    Full Text Available To access the effect of turmeric (Curcuma longa supplementation on growth and blood chemistry of broiler chickens, seventy five day old straight run coloured synthetic broiler chicks were randomly divided into 3 groups (25 chicks per group. Group I served as control (without any supplementation, where as birds in groups II and III were supplemented with 0.5% and 1.0% Curcuma longa powder respectively and the trail was lasted for 7 weeks, during which weekly body weight changes were recorded. Blood samples were collected at the end of the experiment to study the blood profile of birds. The results indicated that addition of Curcuma longa powder caused significant (P0.05 effect on blood biochemical parameters of bird. The present results confirmed the beneficial effects of dietary Curcuma longa powder to improve body weight and Hb concentration of broiler chickens.

  17. EFFECTS OF ARGININE AND VITAMIN E SUPPLEMENTED DIETS ON THE IMMUNOLOGICAL RESPONSE OF BROILERS CHICKENS

    Directory of Open Access Journals (Sweden)

    David Jesús Chan Diaz

    2012-08-01

    Full Text Available In order to evaluate the effect of arginine and vitamin E supplementation in broiler chicken diets on the immune response during post-vaccine stress, a trial was conducted with 700 chicks (1 day-old which were distributed into 28 floor-pens and fed one of four dietary treatments (with 7 replicates randomly assigned: T1 = control diet (1.31 % of arginine and 10 IU of vitamin E/kg of feed; T2 = T1 + 0.3 % of arginine; T3 = T1 + 70 IU of vitamin E; T4 = T1 + 0.3 % of arginine + 70 IU of vitamin E. At 12 days of age, all birds were vaccinated against Newcastle disease virus (ND, infectious bronchitis, avian influenza (AI and fowl pox. The traits evaluated were: post-vaccine reaction at days 14, 16, 18, 21 and 23; antibody titers against ND and AI, and relative lymphoid organs weight at days 11, 19 and 26; and the performance were recorded weekly. Chickens fed T2, T4 (at day 16, and T3 (at day 21 had lesser (p≤0.05 post-vaccine reaction than birds fed T1. The antibody titers against ND (at day 11 was higher (p≤0.05 in chickens fed T4 (3.1, T3 (2.7 and T2 (2.7 compared to T1 (1.6; meanwhile, for AI titers no differences were found. There were no differences, neither for immune organs weight, nor for performance. In conclusion, arginine and vitamin E supplementation in broiler chicken diets reduced the post-vaccine stress and improved the immune response without affecting the performance.

  18. The adrenocortical response of tufted puffin chicks to nutritional deficits

    Science.gov (United States)

    Kitaysky, A.S.; Romano, Marc D.; Piatt, John F.; Wingfield, J.C.; Kikuchi, M.

    2005-01-01

    In several seabirds, nutritional state of a nest-bound chick is negatively correlated with the activity of its hypothalamus-pituitary-adrenal (HPA) axis. Increased corticosterone (cort) secretion has been shown to facilitate changes in behavior that allow hungry chicks to obtain more food from parents. However, if parents are not willing/able to buffer their young from temporary food shortages, increased cort secretion could be detrimental to undernourished chicks. In a system where parents are insensitive to chick demands, low benefits and high costs of activation of the HPA-axis in hungry chicks should lead to a disassociation of the nutritional state of the young and the activity of its HPA-axis. We tested this novel hypothesis for the tufted puffin (Fratercula cirrhata), a seabird with intermittent provisioning of a nest-bound semi-precocial chick. We examined the HPA-axis activity of captive chicks exposed to the following: (1) a short-term (24 h) food deprivation; and (2) an array of prolonged (3 weeks) restrictions in feeding regimens. We found that in response to a short-term food deprivation chicks decreased baseline levels of cort and thyroid hormones. In response to prolonged restrictions, food-limited chicks exhibited signs of nutritional deficit: they had lower body mass, endogenous lipid reserves, and thyroid hormone titers compared to chicks fed ad libitum. However, baseline and maximum acute stress-induced levels of cort were also lower in food-restricted chicks compared to those of chicks fed ad libitum. These results support a major prediction of the study hypothesis that puffin chicks suppress HPA-axis activity in response to short- and long-term nutritional deficits. This physiological adaptation may allow a chick to extend its development in the nest, while eluding detrimental effects of chronic cort elevation. 

  19. Role of batch depletion of broiler houses on the occurrence of Campylobacter spp. in chicken flocks

    DEFF Research Database (Denmark)

    Hald, Birthe; Rattenborg, Erik; Madsen, Mogens

    2001-01-01

    Aims: The effect of batch depletion of broiler houses for campylobacter occurrence in broiler flocks was estimated in 10 flocks, each comprising a separate female and male batch. Methods and Results: The chicks were sampled first bq; cloacal swabs in the broiler houses before the start...... that batch depletion of broiler houses increased the prevalence of Campylobacter spp.-infected broilers in the flocks, that the introduction occurred a hen catching the first batch, and that campylobacter spreads through the entire flock within a week. Significance and Impact of the Study: The results from...

  20. Replacement of soybean meal with babassu meal in rations for broilers from 22 to 42 days old

    Directory of Open Access Journals (Sweden)

    Mônica Calixto da Silva

    2015-04-01

    Full Text Available The objective this work was to evaluate the technical and economic viability of the substitution level of soybean meal by babassu meal in rations to broiler from 22 to 42 days old. It was used 80 male broiler chicks at one day of age, distributed into complete random designs with four treatments (0, 10, 20 e 30% substituition of soybean meal by babassu meal and five repetitions of four broilers each. Were evaluated the performance (weight dain, feed intake and feed conversion, carcass and cuts, organ biometry, feed cost per kilogram body weight and gross margin. To verify the relationship of cost of replacing soybean meal with pie babassu, inequalities were established. The substitution level of soybean meal by babassu meal had no influence (P>0,05 any of the performance characteristics, wich showed the technical viability of substituting up to 30%. Similarly, there was no affect on carcass yield, cuts weight and organ biometry. The cust less with feed per kg for chicken produced and the higher gross margin were obtained from chickens fed diets with 0% babassu meal. The increased of substitution level soybean meal by babassu meal in ration for broilers from 22 to 42 days old proved unviable economically, however, the inequalities produced can be useful in practical situations.

  1. The effects of thermal manipulations during embryogenesis of broiler chicks on growth of embryo and skeletal traits

    Science.gov (United States)

    Aygün, Ali; Narinç, Doǧan

    2016-04-01

    Incubation temperature is one of the important environmental factors that can induce epigenetic thermal adaptation of different physiological control systems. Thus, post hatch thermo tolerance ability of birds may be gained using these manipulations during different incubation periods. The current study was carried out to reveal the effects of temperature manipulations during early and late embryogenesis on weight of embryo and size of skeletal bilateral traits (face, wings, metatarsus, tibia, and femur) in broiler chicken embryos. One thousand commercial broiler eggs from 46 week old breeder flock were used in study. Treatments consisted of eggs incubated at 37.8°C and 55% relative humidity throughout (control; DG1), heated to 36.9°C and supplied 60% relative humidity for 6 hours daily from day 0 to 8 (DG2), heated to 36.9°C and supplied 60% relative humidity for 6 hours daily from day 10 to 18 (DG3), heated to 41°C and supplied 65% relative humidity for 3 hours daily from day 8 to 10 (DG4), and heated to 41°C and supplied 65% relative humidity for 3 hours daily from day 16 to 18 (DG5). Measurements of embryo weight and bilateral traits were obtained at 20 day of incubation and at hatch (at day 21). It was determined that the live weights of embryo and chick were affected significantly by treatment; DG3 group has shown higher mean values than the other treatment groups (Pmetabolic shifts realized by the embryos.

  2. Effect of heating system using a geothermal heat pump on the production performance and housing environment of broiler chickens.

    Science.gov (United States)

    Choi, H C; Salim, H M; Akter, N; Na, J C; Kang, H K; Kim, M J; Kim, D W; Bang, H T; Chae, H S; Suh, O S

    2012-02-01

    A geothermal heat pump (GHP) is a potential heat source for the economic heating of broiler houses with optimum production performance. An investigation was conducted to evaluate the effect of a heating system using a GHP on production performance and housing environment of broiler chickens. A comparative analysis was also performed between the GHP system and a conventional heating system that used diesel for fuel. In total, 34,000 one-day-old straight run broiler chicks were assigned to 2 broiler houses with 5 replicates in each (3,400 birds/replicate pen) for 35 d. Oxygen(,) CO(2), and NH(3) concentrations in the broiler house, energy consumption and cost of heating, and production performance of broilers were evaluated. Results showed that the final BW gain significantly (P heating system did not affect the mortality of chicks during the first 4 wk of the experimental period, but the mortality markedly increased in the conventional broiler house during the last wk of the experiment. Oxygen content in the broiler house during the experimental period was not affected by the heating system, but the CO(2) and NH(3) contents significantly increased (P heating the GHP house was significantly lower (P heating system for broiler chickens.

  3. Evaluation of the addition of organic acids in the feed and/or water for broilers and the subsequent recovery of Salmonella Typhimurium from litter and ceca.

    Science.gov (United States)

    Bourassa, D V; Wilson, K M; Ritz, C R; Kiepper, B K; Buhr, R J

    2018-01-01

    Three separate broiler Salmonella Typhimurium challenge experiments were conducted evaluating efficacy of formic and propionic acid feed supplements to suppress environmental and cecal Salmonella Typhimurium prevalence. In experiment 1, broilers were provided feed with 1 kg/ton formic acid or 5 kg/ton propionic acid feed additives or a basal control diet. At the day of placement, half of the pens were inoculated with seeder chicks orally challenged with a marker strain of Salmonella Typhimurium and to yield challenged and adjacent nonchallenged pens. No differences in weekly litter samples or cecal Salmonella prevalence at 3 or 6 wk among feeding treatments were detected. In experiment 2, treatments were: 2 kg/ton propionic acid in feed, 1.0 mL/L formic acid in water, both propionic acid in feed and formic acid in water, and a basal control. Every pen was challenged with seeder chicks inoculated with Salmonella Typhimurium. By 6 wk all pens maintained detectable litter Salmonella, and broilers provided both propionic acid in feed and formic acid in water had the lowest cecal recovery (35%), compared to the control (60%). In experiment 3, treatments were: formic acid at 4 or 6 kg/ton from wk 0 to 6 or for only the last wk, propionic acid at 5 or 10 kg/ton for only the last wk, and a basal control. Each pen was challenged with Salmonella Typhimurium inoculated seeder chicks. By 6 wk, broilers fed formic acid (4 kg/ton) for the entire growout had no Salmonella-positive ceca (0/30). All treatments that provided acid supplemented feed for only the last wk had 3-13% Salmonella-positive ceca. These experiments indicate that adding formic acid to broiler feed appears to prevent Salmonella colonization from challenge pens entering into the adjacent nonchallenge pens. Feeding formic acid (4 kg/ton) for 6 wk resulted in no recovery of Salmonella from ceca compared to the control prevalence of 17%. Published by Oxford University Press on behalf of Poultry Science

  4. Rearing of germfree chicks in a vinyl isolator

    International Nuclear Information System (INIS)

    Ishibashi, Teru; Kametaka, Masao; Ozaki, Akira; Yamamoto, Tetsuzo; Kaneuchi, Choji.

    1977-01-01

    A method of rearing germfree chicks in a vinyl isolator was developed, Hatchability of eggs was higher than 95% when eggs were sterilized in a 1.5% mercuric chloride solution on the 2nd day before hatching. Sterility was 93% when 5 to 10 chicks were reared in a vinyl isolator for 2 weeks. Depression of body weight gain was observed in chicks fed diet irradiated at higher than 4 Mrad. However, no effects of irradiation on chick growth were observed when fat and the other components were sterilized separately and mixed before feeding. The growth rates of germfree chicks were higher than those of the conventional chicks which were supplied a diluted solution of fresh cecum feces of young hens with a normal intestinal flora pattern at the first feeding. (auth.)

  5. Performance of broiler chicken fed varied nutrient density diets supplemented with direct fed microbial

    Directory of Open Access Journals (Sweden)

    Katoch S.

    2013-01-01

    Full Text Available Comparative dietary response of different isolated strains (Direct fed microbial- DFM of lactobacilli, streptococci and yeast isolated from leopard feces (Panthera leo was studied as probiotic in poultry broiler birds reared as per varied mineral densities viz. calcium and phosphorus. Various treatments consisted of T0 (Control, culture medium, T1 (Lactobacillus casei + Streptococcus fecalis + Saccharomyces cerevisiae offered standard formulated diet D1, T2 - Control (Culture medium offered ten percent lower calcium and phosphorus diet D2, T3 (Lactobacillus casei + Streptococcus fecalis + Saccharomyces cerevisiae offered ten percent lower calcium and phosphorus diet D2. Growth results obtained during the starter phase (1st to 3rd week exhibited higher gain in live body weight and lower feed conversion ratio, both by DFM supplemented treatment T1 offered standard formulated diet D1 and treatment T3 offered experimentally formulated mineral (Ca and P deficient (10% diet D2 compared to respective control T0 and T2. The obtained results revealed a significantly higher growth performance in treatment T1 compared to its control T0 where a higher growth in treatment T3 offered experimentally formulated mineral (Ca and P deficient (10% diet D2 supplemented with isolated DFM compared to its control T2 was exhibited. Treatment groups T1 and T3 supplemented with isolated DFM exhibited a better intestinal micro flora balance, effective colonization and higher count in the intestinal tract with higher calcium and phosphorus retention in the digestibility studies. Thus, it was found that supplementation of isolated DFM has the potential to improve biological growth performance of poultry broiler birds offered both standard formulated diet D1, as well as mineral deficient diet D2. [Projekat Ministarstva nauke Republike Srbije, br. III 46002

  6. Performance of broilers fed during 21 days on mash or pellet diets containing whole or ground pearl millet grain

    Directory of Open Access Journals (Sweden)

    TR Torres

    2013-12-01

    Full Text Available An experiment was carried out to evaluate the effect of the inclusion of 20% whole-grain or ground pearl millet (PM in mash and pelleted diets on the performance, carcass traits, and organ weights of broilers reared until 21 days of age. A randomized block experimental design in a 3 x 2 factorial arrangement (diets containing corn and soybean meal, whole-grain PM, or ground PM x mash or pelleted diets, with five replicates per treatment and 10 birds per experimental unit, was applied. Diets were analyzed for mean geometric diameter, geometric standard deviation, pellet hardness, and density. Broiler performance, carcass yield, and organ weights were evaluated. On day 21, one bird with the average weight of each experimental unit was sacrificed for carcass evaluation. It was concluded that both as whole-grain and ground PM can be added to the diet of broilers up to 21 days of age. The dietary inclusion of PM results in higher abdominal fat deposition. Broilers fed the pelleted diets presented lower feed intake, better feed conversion ratio, lower gizzard and heart percentages, and higher carcass weight.

  7. Effect of refined functional carbohydrates from enzymatically hydrolyzed yeast on the presence of Salmonella spp. in the ceca of broiler breeder females.

    Science.gov (United States)

    Walker, G K; Jalukar, S; Brake, J

    2017-08-01

    Broiler breeders hatched from Salmo-nella negative grandparents received either zero or 50 g/MT of refined functional carbohydrates (RFC) in their diets from d of placement to end of lay. There were no other treatments used. Pullets and cockerels were reared separately in an enclosed litter-floor house to 21 wk of age when 28 randomly selected pullets from each diet were transferred to individual cages for an additional 14 d before they were killed, and their ceca were excised aseptically and tested for Salmonella spp. The remaining birds were transferred to a two-thirds slat and one-third litter curtain-sided laying house. There were 8 pens of 60 to 65 females and 8 to 18 males, depending upon flock age and housing type, fed each diet, and there was no effort made to isolate pens from typical daily foot traffic between pens. At 51 wk of age, male progeny broiler chicks were hatched and received either zero or 50 g/MT of RFC to complete a 2 × 2 design with 4 replicate pens of 12 males per interaction. All broilers were tested for cecal Salmonella spp. at 34 d of age. Ceca were collected from 30 breeder hens from each treatment at 64 wk of age and tested for Salmonella spp. Of the ceca sampled at 23 wk from the control pullets, 71.4% were found to contain Salmonella spp., while none of the ceca from the RFC pullets tested positive. Of the ceca sampled from the control hens at 64 wk, 40% were found to contain Salmonella spp., while none of the ceca from the RFC hens tested positive. Salmonella spp. was isolated from broilers in one pen of the control broilers that were also progeny of control breeders out of 4 replicates but not from any pens in which the breeders had been fed RFC. These data demonstrated that RFC reduced natural Salmonella spp. colonization of broiler breeder hen and broiler progeny ceca during a complete production cycle. © 2017 Poultry Science Association Inc.

  8. Effect of probiotics on broiler meat quality | Kabir | African Journal of ...

    African Journals Online (AJOL)

    The study was carried out on “Hubbard Isa Starbo” broilers to evaluate the raw meat quality of both probiotics and conventional fed broilers in pre-freezing condition and ... The groups C and D were taken as control birds and were fed with commercial ration and the groups A and B as experimental birds which were fed with ...

  9. Dietary supplementation of a mixture of Lactobacillus strains enhances performance of broiler chickens raised under heat stress conditions

    Science.gov (United States)

    Faseleh Jahromi, Mohammad; Wesam Altaher, Yassir; Shokryazdan, Parisa; Ebrahimi, Roohollah; Ebrahimi, Mahdi; Idrus, Zulkifli; Tufarelli, Vincenzo; Liang, Juan Boo

    2016-07-01

    High ambient temperature is a major problem in commercial broiler production in the humid tropics because high producing broiler birds consume more feed, have higher metabolic activity, and thus higher body heat production. To evaluate the effects of two previously isolated potential probiotic strains ( Lactobacillus pentosus ITA23 and Lactobacillus acidophilus ITA44) on broilers growing under heat stress condition, a total of 192 chicks were randomly allocated into four treatment groups of 48 chickens each as follows: CL, birds fed with basal diet raised in 24 °C; PL, birds fed with basal diet plus 0.1 % probiotic mixture raised in 24 °C; CH, birds fed with basal diet raised in 35 °C; and PH, birds fed with basal diet plus 0.1 % probiotic mixture raised in 35 °C. The effects of probiotic mixture on the performance, expression of nutrient absorption genes of the small intestine, volatile fatty acids (VFA) and microbial population of cecal contents, antioxidant capacity of liver, and fatty acid composition of breast muscle were investigated. Results showed that probiotic positively affected the final body weight under both temperature conditions (PL and PH groups) compared to their respective control groups (CL and CH). Probiotic supplementation numerically improved the average daily gain (ADG) under lower temperature, but significantly improved ADG under the higher temperature ( P < 0.05) by sustaining high feed intake. Under the lower temperature environment, supplementation of the two Lactobacillus strains significantly increased the expression of the four sugar transporter genes tested (GLUT2, GLUT5, SGLT1, and SGLT4) indicating probiotic enhances the absorption of this nutrient. Similar but less pronounced effect was also observed under higher temperature (35 °C) condition. In addition, the probiotic mixture improved bacterial population of the cecal contents, by increasing beneficial bacteria and decreasing Escherichia coli population, which could be

  10. Effects of dietary acidifer-based diet on haematology and serum ...

    African Journals Online (AJOL)

    A trial was conducted to determine the effect of dietary Biotronics SE® supplementation on haematological and serum parameters of broilers. A total of 120 1-day old Marshall broilers were randomly distributed to four dietary treatments using completely randomized design. Chicks in treatment 1 were fed basal diet (control) ...

  11. Effects of high grade bentonite on performance, organ weights and serum biochemistry during aflatoxicosis in broilers

    Directory of Open Access Journals (Sweden)

    H. C. Indresh

    2013-12-01

    Full Text Available Aim: To evaluate the effect of different levels of High Bentonite on growth performance, organ weight and serum biochemistry in broiler fed on diets containing aflatoxin. Materials and Methods: A total of 360 day-old commercial broiler chicks were divided at random into 8 dietary treatment groups of 42 chicks each having 3 replicates. Dietary levels of aflatoxin (0.5 ppm and High-grade bentonite (0.5, 0.75 and 1.00% were tested in a completely randomized design manner, forming a total of 8 dietary treatments each with three replicates. Body weight and feed intake were recorded weekly. At 5 wk, six birds from each treatment were sacrificed and liver, kidney, gizzard, pancreas, spleen, bursa of Fabricius and thymus were extracted and weighed. The serum samples were analyzed for total proteins, uric acid, serum albumin, serum globulin and the activities of gamma glutamyl transferase (GGT, alanine amino transferase (ALT and for antibody titers against Newcastle disease (ND and infectious bursal disease (IBD using ELISA technique. Results: A significant (P<0.05 decrease in body weight, feed consumption, relative weights of bursa, thymus, serum protein, anti body titers against NDV and IBDV, and increase in FCR, mortality, relative weight of liver, kidney, and the activity of Gamma Glutamyl Transferase (GGT was observed. However, the relative weights of gizzard, pancreas, spleen, serum albumin, uric acid and the activity of Alanine Amino Transferase (ALT were not influenced by inclusion of AF or HGB. Conclusion: The addition of HGB restored the harmful effects of AF on body weight, feed consumption, FCR, mortality, relative weight of liver, kidney, serum protein, IBDV and NDV. Supplementation of high grade bentonite at 1.0 per cent level was found to be beneficial in ameliorating the adverse effects of aflatoxin (AF in broiler chickens. [Vet World 2013; 6(6.000: 313-317

  12. Growth performance and gastrointestinal responses of broiler chickens fed corn-soybean meal diet without or with exogenous epidermal growth factor upon challenge with Eimeria.

    Science.gov (United States)

    Kim, E; Leung, H; Akhtar, N; Li, J; Barta, J R; Wang, Y; Yang, C; Kiarie, E

    2017-10-01

    Epidermal growth factor (EGF), a protein known for its mitogenic and anti-apoptotic effects was fed to broiler chickens to evaluate growth performance, gastrointestinal measurements, and apparent retention (AR) of components upon challenge with Eimeria. A total of 216, d old male broiler chicks (Ross 708) were placed in cages (6 birds/cage) and allocated to treatments. The treatments were: 1) control (Lactotobacilli lactis fermentation supernatant without EGF), 2) 80 μg of EGF/kg BW/d, and 3) 160 μg of EGF/kg BW/d. A basal antibiotic-free corn-soybean diet containing TiO2 was used. Birds were offered fresh feed with respective treatments on daily basis and had free access to drinking water for 14 d. On d 5, birds (6 replicates per treatment) were challenged with 1 mL of E. acervulina and E. maxima mixture via oral gavage and the other 6 replicates were given sham. Growth performance was measured in pre- (d 0 to 5) and post- (d 6 to 14) challenge periods. Two birds per cage were necropsied on d 10 for intestinal lesion scores and tissue samples for histomorphology and expression of select intestinal genes. Excreta samples for AR of components and oocyst shedding were taken d 10 to 13 and all birds were necropsied on d 14 for gastrointestinal weight. The EGF linearly (P Eimeria interaction (P > 0.05) on growth performance, AR of GE, and intestinal histomorphology; the main effects were such that Eimeria depressed (P Eimeria (P Eimeria challenged birds whilst no effect in non-challenged control. In conclusion, Eimeria challenge reduced growth performance and impaired gut function; EGF showed beneficial effects on growth pre-challenge and improved indices of gut function upon Eimeria challenge. © The Author 2017. Published by Oxford University Press on behalf of Poultry Science Association.

  13. Impact of extruded flaxseed meal supplemented diet on growth performance, oxidative stability and quality of broiler meat and meat products

    Directory of Open Access Journals (Sweden)

    Anjum Faqir Muhammad

    2013-02-01

    Full Text Available Abstract This study was intended to explore the effect of extruded flaxseed meal supplemented diet on broiler growth performance, oxidative stability and organoleptic characteristics of broiler meat and meat products. 120 (day old broiler chicks were randomly allotted to 12 experimental groups and fed on diets containing extruded flaxseed meal at 0, 5, 10 and 15%. The supplementation of extruded flaxseed in the diet decreases the body weight gain, feed intake and increased feed conversion ratio (FCR values of broilers. The antioxidant enzymes were strongly influenced by different levels of extruded flaxseed supplementation among treatments. The TBARS assay revealed that maximum malondialdehyde were produced in T3 containing highest extruded flaxseed level (15% and minimum malondialdehyde were produced in T0 treatment having no extruded flaxseed. The TBARS values ranged from 0.850-2.106 and 0.460-1.052 in leg and breast met respectively. The Free radical scavenging activity varied significantly and DPPH values of breast meat ranged from 20.70% to 39.09% and in leg meat 23.53% to 43.09% respectively. The sensory acceptability of broiler meat nuggets was decreased with the increase in the level of flaxseeds due to the lipid peroxidation of polyunsaturated fatty acids (PUFA which generated off flavors and bad odors. Feeding extruded flaxseed to chicken through feed strongly inflated the quality and functional properties, fatty acid contents and reduced the oxidative stability of broiler meat and meat products. The present study concludes that up to 10% of flaxseed meal may be used in broiler diet to enhance the omega 3 fatty acids content in the broiler meat.

  14. Impact of extruded flaxseed meal supplemented diet on growth performance, oxidative stability and quality of broiler meat and meat products.

    Science.gov (United States)

    Anjum, Faqir Muhammad; Haider, Muhammad Faizan; Khan, Muhammad Issa; Sohaib, Muhammad; Arshad, Muhammad Sajid

    2013-02-08

    This study was intended to explore the effect of extruded flaxseed meal supplemented diet on broiler growth performance, oxidative stability and organoleptic characteristics of broiler meat and meat products. 120 (day old) broiler chicks were randomly allotted to 12 experimental groups and fed on diets containing extruded flaxseed meal at 0, 5, 10 and 15%. The supplementation of extruded flaxseed in the diet decreases the body weight gain, feed intake and increased feed conversion ratio (FCR) values of broilers. The antioxidant enzymes were strongly influenced by different levels of extruded flaxseed supplementation among treatments. The TBARS assay revealed that maximum malondialdehyde were produced in T3 containing highest extruded flaxseed level (15%) and minimum malondialdehyde were produced in T0 treatment having no extruded flaxseed. The TBARS values ranged from 0.850-2.106 and 0.460-1.052 in leg and breast met respectively. The Free radical scavenging activity varied significantly and DPPH values of breast meat ranged from 20.70% to 39.09% and in leg meat 23.53% to 43.09% respectively. The sensory acceptability of broiler meat nuggets was decreased with the increase in the level of flaxseeds due to the lipid peroxidation of polyunsaturated fatty acids (PUFA) which generated off flavors and bad odors. Feeding extruded flaxseed to chicken through feed strongly inflated the quality and functional properties, fatty acid contents and reduced the oxidative stability of broiler meat and meat products. The present study concludes that up to 10% of flaxseed meal may be used in broiler diet to enhance the omega 3 fatty acids content in the broiler meat.

  15. Effects of Dietary Zinc Pectin Oligosaccharides Chelate Supplementation on Growth Performance, Nutrient Digestibility and Tissue Zinc Concentrations of Broilers.

    Science.gov (United States)

    Wang, Zhongcheng; Yu, Huimin; Wu, Xuezhuang; Zhang, Tietao; Cui, Hu; Wan, Chunmeng; Gao, Xiuhua

    2016-10-01

    The experiment was conducted to investigate the effects of zinc pectin oligosaccharides (Zn-POS) chelate on growth performance, nutrient digestibility, and tissue zinc concentrations of Arbor Acre broilers aged from 1 to 42 days. A total of 576 1-day-old broilers were randomly assigned into 4 groups with 9 replicates per group and 16 chicks per replicate. Chicks were fed either a basal diet (control) or basal diet supplemented with Zn-POS at 300 (Zn-POS-300), 600 (Zn-POS-600), or 900 mg/kg (Zn-POS-900), respectively, for 42 days. A 3-day metabolism trial was conducted during the last week of the experiment feeding. The average daily gain and the average daily feed intake of Zn-POS-600 were significantly higher (P digestibility of dry matter, crude protein, and metabolic energy among all groups. The control group had the lowest apparent digestibility of dry matter (P digestibility of dry matter in Zn-POS-600 was higher (P digestibility of crude protein in Zn-POS-600 or Zn-POS-900 was higher (P digestibility of metabolic energy in Zn-POS-600 or Zn-POS-900 was higher (P < 0.05) than that of Zn-POS-300. Zn-POS-600 had the highest liver zinc concentrations (P < 0.05), while Zn-POS-900 had the highest pancreatic zinc concentrations (P < 0.05). Our data suggest that the supplementation of 600 mg/kg Zn-POS is optimal in improving the average daily gain and the average daily feed intake, utilization of dietary dry matter and crude protein, and increasing tissue zinc concentrations in liver and pancreas of broilers.

  16. Pro biotic as Alternative to Antibiotic for Broiler Chicken fed Food Industrial Residual Oil

    International Nuclear Information System (INIS)

    EL-Faramawy, A.A.; El-Maghraby, A.F.; El-Danasoury, M.M.; Hussien, H.A.; Hegazy, E.S.

    2016-01-01

    This study aimed to evaluate the effect of pro biotic (some lactic acid bacteria) with different levels of food industrial residual oil in broiler commercial diets on growth performance, meat yield, internal organs, economical efficiency and performance index. One hundred and eighty one day old Cobb chicks (45 ± 0.4 g) were equally and randomly divided into 6 groups namely; the antibiotic with fresh oil (FO), the antibiotic with mixed oil (MO) [FO+RO ( 1:1 w/w)], the antibiotic with food industrial residual oil (RO), the pro biotic with FO, the pro biotic with MO and the pro biotic with RO. Virginiamycin, Phibro, USA (15 ppm), was the antibiotic, while a mixture of lactic acid bacteria is chosen as pro biotic. Both were added to the water. During the experiment which lasted for 42 days, the body weight, the feed intake and the mortality rate were recorded at 2, 4 and 6 weeks of age then the body weight gain, feed conversion ratio, economical efficiency and performance index were calculated. The results revealed that the average body weight, body weight gain and feed consumption significantly (P 0.05) while liver increased significantly (P<0.05) in pro biotic FO and gizzard in all pro biotic group and antibiotic MO. The highest performance index was observed in groups of birds treated with pro biotic with MO followed by birds treated with pro biotic FO without significant difference. It could be concluded that supplementation of pro biotic in broiler diet containing different levels of RO was economically more beneficial than antibiotic

  17. Chelating effect of silver nitrate by chitosan on its toxicity and growth performance in broiler chickens

    Directory of Open Access Journals (Sweden)

    Yemdjie Mane Divine Doriane

    2017-06-01

    Full Text Available Objective: This study was conducted to investigate the chelating effect of silver nitrate (AgNO3 by chitosan on growth performances, hematological and biochemical parameters, and the histopathological structure of the liver and the kidney in broiler chicken. Materials and methods: A total of 192 day-old Cobb 500 strain chicks were randomly assigned to 3 treatments of 64 chicks each. Control group was fed on basal diet without supplement (R0 and the two others groups were fed on rations supplemented with 10 mg of unchelated (RAg or chelated (RCs-Ag AgNO3 per Kg of feed, respectively. Parameters that have been studied consisted of feed intake, weight gain, blood and serum biochemical, and histopathological analyses of liver and kidney. Results: Results revealed that chelation of AgNO3 by chitosan did not have any effect on growth performances and hematological parameters in chicken. However, chelated and unchelated AgNO3 increased the serum content in triglyceride, and cholesterol and decreased the serum content in creatinin, albumin and alanine aminotransferase (ALAT. Chelating AgNO3 with chitosan prevented and corrected the toxicity induced on the histological structure of liver and kidney. Conclusion: Chitosan can be used as a chelating agent to alleviate the harmful effects of AgNO3 as silver ion for poultry. [J Adv Vet Anim Res 2017; 4(2.000: 187-193

  18. Performance of broiler chickens fed diets supplemented with feed ...

    African Journals Online (AJOL)

    Avizyme 1500TM) on the performance and nutrient digestibility by broiler finishers was investigated. A total of 120 d-old broiler chickens was assigned to four treatments each having two replicates of 15 birds in a 2 x 2 factorial block design.

  19. Arsenic induced clinico-hemato-pathological alterations in broilers and its attenuation by vitamin e and selenium

    International Nuclear Information System (INIS)

    Mashkoor, J.; Khan, A.; Khan, M.Z.; Saleemi, M.K.; Mahmood, F.

    2012-01-01

    Present study was carried out to know the arsenic (As) induced toxico-pathological alterations in broiler chicks and their attenuation with vitamin E (Vit E) and selenium (Se). A total of 90 day-old broiler chicks were equally distributed into 5 groups. Groups 1-4 were administered As at 50 mg/kg BW daily through feed for 30 days. In addition to A , groups 2 to 4 received Vitamin E at 150 mg/kg BW, selenium at 0.25 mg/kg BW and Vitamin E plus selenium, respectively. Group 5 (Control) received normal drinking water for 30 days. Dullness, depression, open mouth breathing, increased thirst; ruffled feathers, pale comb, skin irritation and watery diarrhea were the most striking clinical signs. The body weight and feed intake was significantly decreased in treated birds. The erythrocyte counts, hemoglobin concentration and packed cell volume decreased (P<0.05) in treated broilers with As or As with Se and Vit E. Grossly pale and hemorrhagic liver and swollen kidneys were observed in As treated birds. Arsenic treated groups showed significant decrease in serum. Histopathologically, liver exhibited congestion and cytoplasmic vacuolation. In kidneys, condensation of tubular epithelium nuclei, epithelial cell necrosis, increased urinary spaces, sloughing of tubules from basement membrane and cast deposition were observed. In conclusion As induced toxico-pathological alterations and vitamin E and selenium partially ameliorate the toxic effects in broilers chicks. (author)

  20. Prebiotic, probiotic and symbiotic as alternative to Antibiotics on the Performance and Immune Response of Broiler Chickens

    Directory of Open Access Journals (Sweden)

    VDA Murarolli

    2014-09-01

    Full Text Available This study aimed to evaluate diets supplemented with prebiotic, probiotic and symbiotic as an alternative to antibiotics on the performance and immune response against the virus of Newcastle disease in broiler chickens. 1,400 one-day old male Cobb 500 chicks were raised until 42 days old in a completely randomized design with 2x2+1 factorial scheme with seven replications. The treatments were: diet without supplementation (base diet - BD, BD + prebiotic, BD + probiotic, BD + symbiotic (prebiotic + probiotic, and BD + antibiotic. The parameters evaluated were performance and antibody serum titers against Newcastle disease. No antibiotic effect was observed on performance. The symbiotic provided better results for weight gain and feed:gain ratio until 21 days old than isolated additives. At 28 days old, the broilers fed diets with prebiotic presented better feed: gain ratio. In the same period (28 d-old, there was an antibody production increase against the Newcastle disease virus in the group supplemented with prebiotic. It can be concluded that the utilization of symbiotic in broiler chickens' diets can substitute performance enhancing antibiotics. The inclusion of prebiotic in the diet improves feed: gain ratio at 1-28 days old. The chickens' immune response increases at 28 days against the Newcastle disease virus in the group supplemented with prebiotic.

  1. Effects of feeding blends of grains naturally contaminated with Fusarium mycotoxins on growth and immunological parameters of broiler chickens.

    Science.gov (United States)

    Swamy, H V L N; Smith, T K; Karrow, N A; Boermans, H J

    2004-04-01

    An experiment was conducted to investigate the effects of feeding grains naturally contaminated with Fusarium mycotoxins on growth and immunological parameters of broiler chickens. Three hundred sixty, 1-d-old male broiler chicks were fed 1 of 4 diets containing grains naturally contaminated with Fusarium mycotoxins for 56 d. The diets included (1) control; (2) low level of contaminated grains (5.9 mg/kg deoxynivalenol (DON), 19.1 mg/kg fusaric acid (FA), 0.4 mg/kg zearalenone, and 0.3 mg/kg 15-acetyldeoxynivalenol; (3) high level of contaminated grains (9.5 mg/kg DON, 21.4 mg/kg FA, 0.7 mg/kg zearalenone, and 0.5 mg/kg 15-acetyldeoxynivalenol); and (4) high level of contaminated grains + 0.2% polymeric glucomannan mycotoxin adsorbent (GM polymer). Body weight gains and feed consumption of chickens fed contaminated grains decreased linearly with the inclusion of contaminated grains during the grower phase (d 21 to 42). Efficiency of feed utilization, however, was not affected by diet. Production parameters were not significantly affected by the supplementation of GM polymer to the contaminated grains. Peripheral blood monocytes decreased linearly in birds fed contaminated grains. The feeding of contaminated diets linearly reduced the B-cell count at the end of the experiment, whereas the T-cell count on d 28 responded quadratically to the contaminated diets. The feeding of contaminated diets did not significantly alter serum or bile immunoglobulin concentrations, contact hypersensitivity to dinitrochlorobenzene, or antibody response to SRBC. Supplementation with GM polymer in the contaminated diet nonspecifically increased white blood cell count and lymphocyte count, while preventing mycotoxin-induced decreases in B-cell counts. It was concluded that broiler chickens are susceptible during extended feeding of grains naturally contaminated with Fusarium mycotoxins.

  2. Growth performance and gastrointestinal responses of broiler chickens fed corn-soybean meal diet without or with exogenous epidermal growth factor upon challenge with Eimeria1

    Science.gov (United States)

    Kim, E.; Leung, H.; Akhtar, N.; Li, J.; Barta, J. R.; Wang, Y.; Yang, C.; Kiarie, E.

    2017-01-01

    Abstract Epidermal growth factor (EGF), a protein known for its mitogenic and anti-apoptotic effects was fed to broiler chickens to evaluate growth performance, gastrointestinal measurements, and apparent retention (AR) of components upon challenge with Eimeria. A total of 216, d old male broiler chicks (Ross 708) were placed in cages (6 birds/cage) and allocated to treatments. The treatments were: 1) control (Lactotobacilli lactis fermentation supernatant without EGF), 2) 80 μg of EGF/kg BW/d, and 3) 160 μg of EGF/kg BW/d. A basal antibiotic-free corn-soybean diet containing TiO2 was used. Birds were offered fresh feed with respective treatments on daily basis and had free access to drinking water for 14 d. On d 5, birds (6 replicates per treatment) were challenged with 1 mL of E. acervulina and E. maxima mixture via oral gavage and the other 6 replicates were given sham. Growth performance was measured in pre- (d 0 to 5) and post- (d 6 to 14) challenge periods. Two birds per cage were necropsied on d 10 for intestinal lesion scores and tissue samples for histomorphology and expression of select intestinal genes. Excreta samples for AR of components and oocyst shedding were taken d 10 to 13 and all birds were necropsied on d 14 for gastrointestinal weight. The EGF linearly (P Eimeria interaction (P > 0.05) on growth performance, AR of GE, and intestinal histomorphology; the main effects were such that Eimeria depressed (P Eimeria (P Eimeria challenged birds whilst no effect in non-challenged control. In conclusion, Eimeria challenge reduced growth performance and impaired gut function; EGF showed beneficial effects on growth pre-challenge and improved indices of gut function upon Eimeria challenge. PMID:28938785

  3. Effect of commercial diets quality on bio-economic performances of broilers in Benin

    DEFF Research Database (Denmark)

    Houndonougbo, F M; Chwalibog, André; Chrysostome, C A A M

    2009-01-01

    The objective of this study was to assess the quality of commercial poultry feeds in Benin. The performances of 396 unsexed broilers chickens Ross 308 fed with a control diet (R1) and five commercial diets (R2 to R6) were evaluated. Broilers fed commercial diets showed significantly low (P ....001) body weight gain (BWG) and economic feed efficiency (EFE) and significantly high (P cost (FC). At 42 days of age, the body weight of broilers fed control diet was 1662 g versus 838 to 1041 g for broilers fed commercial diets. In R1 diet, overall FC...... commercial diets. These results suggest the necessity to organize the market of poultry feed in Benin in the perspective to reduce the production cost by using more efficient and cheap commercial diets....

  4. The efficacy of a commercial competitive exclusion product on Campylobacter colonization in broiler chickens in a 5-week pilot-scale study.

    Science.gov (United States)

    Schneitz, C; Hakkinen, M

    2016-05-01

    The efficacy of the commercial competitive exclusion product Broilact against Campylobacter jejuni was evaluated in broiler chickens in a 5-week pilot-scale study. Newly-hatched broiler chicks were brought from a commercial hatchery. After arrival 50 seeder chicks were challenged orally with approximately 10(3) cfu of C. jejuni, wing marked, and placed back in a delivery box and moved to a separate room. The rest of the chicks (contact chicks) were placed in floor pens, 100 chicks per pen. Birds in two pens were treated orally on the day of hatch with the commercial competitive exclusion (CE) product Broilact, and three pens were left untreated. The following day 10 seeder chicks were introduced into the Broilact treated and untreated control pens. One pen was left both untreated and unchallenged (0-control). Each week the ceca of 10 contact chicks and one seeder chick were examined quantitatively for Campylobacter The treatment prevented or significantly reduced the colonization of the challenge organism in the ceca during the two first weeks; the percentage of colonized birds being 0% after the first week and 30% after the second week in the Broilact treated groups but was 100% in the control groups the entire 5-week rearing period. During the third rearing week the proportion of Campylobacter positive birds started to increase in the treated pens, being 80% after the third week and 95 and 90% after the fourth and fifth rearing weeks, respectively. Similarly the average count of Campylobacter in the cecal contents of the Broilact treated chicks started to increase, the difference between the treated and control chicks being 1.4 logs at the end of the rearing period. Although the protective effect was temporary and occurred only during the first two weeks of the rearing period, the results of this study support the earlier observations that CE flora designed to protect chicks from Salmonella may also reduce Campylobacter colonization of broiler chickens. © The

  5. THE INFLUENCE OF REARING SYSTEM ON SKIN COLOUR IN BROILERS

    Directory of Open Access Journals (Sweden)

    Dušan Terčič

    2000-06-01

    Full Text Available The aim of this study was to determine the effect of different rearing systems on the skin colour of commercial broilers. Research was carried out on 100 broilers of two different provenances (ross 208 and prelux-bro up to 56 days of age. In the first half of the experiment the broilers were fed according to standard technology with bro starter which contained 23.44 % crude protein and 12.98 MJ/kg metabolizable energy. On the 28th day broilers were divided into two groups and fed with bro finisher which contained 70 % cereals, 14.0 % crude protein and 16.26 MJ/kg metabolizable energy. Half of the broilers were kept indoors without access to the grassland, while the other half had free access during the day (12 hours. Free range broilers showed a higher degree of pigmentation in skin colour than the broilers in confinement. The differences were statistically significant for the L* (lightness and b* (yellowness values.

  6. A questionnaire-based, retrospective field study of persistence of Salmonella Enteritidis and Salmonella Typhimurium in Danish broiler houses

    DEFF Research Database (Denmark)

    Gradel, K.O.; Rattenborg, Erik

    2003-01-01

    A questionnaire-based, retrospective field study was conducted in 78 Danish broiler houses (analytical units) on 42 farms. In spring 1997, all these broiler houses had been infected with Salmonella Enteritidis, phage type 8, and/or Salmonella Typhimurium, definitive-type 66, by day-old chicks del...... soap and water for washing hands in the anteroom, hygiene barriers when removing dead broilers, gravel alongside the broiler house, systematic checks of indoor rodent-bait depots, and combined surface and pulse-fogging disinfection....

  7. Contaminated Larval and Adult Lesser Mealworms, Alphitobius diaperinus (Coleoptera: Tenebrionidae)can Transmit Salmonella Typhimurium in a Broiler Flock

    Science.gov (United States)

    The ability of the lesser mealworm, Alphitobius diaperinus (Panzer), commonly known as the darkling beetle, to transmit a marker strain Salmonella Typhimurium to day-of-hatch broiler chicks was evaluated, as well as the spread to non-challenged pen mates. Day-of-hatch chicks were orally gavaged wit...

  8. INCUBATION YIELD AS A FUNCTION OF BROILER BREEDER AGE / RENDIMENTO DE INCUBAÇÃO EM FUNÇÃO DA IDADE DA MATRIZ

    Directory of Open Access Journals (Sweden)

    G. F. da Silva

    2017-09-01

    Full Text Available The factors involved in poultry egg incubation include breeder hen age, which may influence both the external and internal quality of eggs. In this context, this study sought to determine incubation yield among eggs from breeder hens at three ages. A total of 1,728 eggs were obtained from Cobb broiler breeders at three ages (26 weeks, 32 weeks, and 53 weeks and incubated. At the end of the incubation process, eclosion, hatchability, the percentage of infertile eggs, the time of embryo mortality, and chick weight were all analyzed according to breeder hen age. Analysis of variance was applied to the results, and egg fertility was found to be associated with breeder age, since lower rates of eclosion were found in eggs from younger broiler breeders. Chick weight was also found to be influenced by breeder age, since eggs from older broiler breeders hatched into heavier chicks. However, neither mortality nor hatchability were found to be affected by breeder hen age.

  9. A comparison between a yeast cell wall extract (Bio-Mos® and palm kernel expeller as mannan-oligosaccharides sources on the performance and ileal microbial population of broiler chickens

    Directory of Open Access Journals (Sweden)

    Bahman Navidshad

    2015-02-01

    Full Text Available The present study was conducted to determine the effect of a yeast cell wall extract (Bio- Mos and palm kernel expeller (PKE on the performance, nutrient digestibility, and ileal bacteria population of broiler chickens. A total of 60 1-d-old male broiler chicks (Cobb 500 were fed one of the 3 isonitrogenous and isocaloric diet including a control diet, or a control diet supplemented with 2 g/kg Bio-Mos (1-42 d, and for the third group, the control diet at 1-28 d following a diet containing 200 g/kg of an enzymatically-treated PKE at 29-42 d. The weight gains of birds fed the PKE containing diet (96.17 g/d were less than other groups (109.10 and 104.42 g/d for the Bio-Mos and control diet, respectively (P0.05, but the birds fed PKE or Bio-Mos containing diets had a lower population of Escherichia coli than the control group (P<0.05. The results showed that PKE potentially has a prebiotic property for chicken; however, a 200 g/kg dietary inclusion rate of PKE is not commercially recommendable because of its negative effects on the nutrients digestibility.

  10. Effects of house type and early feed restriction on performance and fat deposition in unsexed broilers

    Directory of Open Access Journals (Sweden)

    U Santoso

    2002-06-01

    Full Text Available The present experiment was conducted to evaluate the effects of house type and early feed restriction on performance and fat deposition of unsexed broilers. Four hundreds seven-day old unsexed broilers (Arbor Acres CP 707 were distributed into eight treatment groups. Each treatment group was represented by five replicates of ten broilers each. Two types of house (cage vs litter and four levels of feeding (ad lib., 75% ad lib., 50% ad lib. and 25% ad lib. were tested as treatment factors. Broilers were feed-restricted for 6 days from 7 to 13 days of age and thereafter they were fed ad lib. Feed intake of restricted broilers during restriction period was calculated from feed consumed by ad lib. group in the previous day. Results showed that unsexed broilers raised in litter had higher body weight (P<0.05, lower abdominal fat and higher triglyceride concentration at 42 days of age (P<0.05, and lower liver fat and higher carcass percentage at 56 days of age (P<0.05. Early feed restriction reduced body weight of 42-day old unsexed broilers except for broilers fed 75% ad lib. At 56 days of age, restricted broilers had similar body weight to those fed ad lib. At 56 days of age, broilers fed 25% ad lib. had lower FCR (P<0.05, lower abdominal fat (P<0.05 and lower triglyceride concentration (P<0,05. In conclusion, unsexed broilers fed 25% ad lib. showed compensatory growth with better FCR and lower fat accumulation at 56 days of age. Broilers raised in litter had higher body weight and lower abdominal and liver fat deposition.

  11. Serum biochemical indices of Finisher Broiler Chickens fed diets ...

    African Journals Online (AJOL)

    A 4 week feeding trial was conducted using 180 five-week old Hubbard broiler chickens to determine the effect of feeding variously processed roselle(Hibiscus sabdariffa) seeds on serum biochemical indices with a view to determining the potential of roselle seed as an alternative to soybeans. Five broiler finisher diets were ...

  12. Enzimas exógenas em dietas de frangos de corte: desempenho Exogenous enzymes in broilers fed diets: performance

    Directory of Open Access Journals (Sweden)

    Nei André Arruda Barbosa

    2012-08-01

    Full Text Available O experimento foi conduzido para avaliar a eficiência de enzimas exógenas em dietas sobre o desempenho de frangos de corte. Um total de 1440 pintos de corte machos (Cobb 500® foram distribuídos em um delineamento inteiramente casualizado em esquema fatorial 2x2 (duas dietas com diferentes densidades nutricionais; com e sem a adição enzimática com 8 repetições de 45 aves em cada unidade experimental. As dietas com diferentes densidades nutricionais foram: controle positivo (níveis normais e um controle negativo (com redução de 4,3 e 4,5% de energia metabolizável; 16,7 e 17,7% de cálcio e 35 e 42,7% de fósforo, nas fases inicial e de crescimento, respectivamente. A suplementação enzimática consistiu da combinação das enzimas fitase (100g t-1 e amilase, xilanase e protease (500g t-1. Foi avaliado o desempenho das aves nas fases de 1 a 21 e 1 a 42 dias de idade. Na fase total de criação, as aves alimentadas com a dieta controle negativo com adição enzimática tiveram consumo de ração (5,97%, peso médio (8,47%, ganho de peso (8,64% e conversão alimentar (2,92% melhores (P0,05 ao grupo alimentado com a dieta controle positivo com ou sem enzima. A adição de enzimas exógenas em dieta de frangos de corte com redução de energia metabolizável, cálcio e fósforo, proporciona um consumo de ração, peso vivo médio e ganho de peso similar a uma dieta com níveis nutricionais adequados.The experiment was conducted to evaluate the effect of enzyme efficiency in diets with and without nutrient reduction on the performance of broilers. Thousand and hundred forty male broiler chicks (Cobb 500® were distributed in a completely randomized design with 4 treatments in a factorial 2x2 (two diets with different nutrient densities, with and without enzyme with 8 replications of 45 birds. The diets were: positive control (normal and a negative control (with a reduction of 4.3% and 4.5% of metabolizable energy, 16.7% and 17.7% of

  13. Subsequent growth performance and digestive physiology of broilers fed on starter diets containing spray-dried porcine plasma as a substitute for meat meal.

    Science.gov (United States)

    Beski, S S M; Swick, R A; Iji, P A

    2015-01-01

    A 4 × 2 factorial experiment was conducted to investigate the effect of inclusion of spray-dried porcine plasma (SDPP), in lieu of meat meal, in the starter diet on performance and digestive physiology of broiler chickens between hatch and 35 d of age. Four levels of SDPP (0, 5, 10 or 20 g/kg) were included in the starter diets in lieu of meat meal on either wheat- or maize-based diets. Over the first 10 d, and throughout the 35-d experimental period, birds gained more body weight with increasing concentrations of SDPP regardless to the type of grain used. Inclusion of SDPP in the starter diet markedly improved feed per gain in the starter phase and across the 35-d study. There was no significant effect of the type of grain and its interaction with SDPP on the body weight gain and feed per gain for the two assessed periods. At d 10, the relative weight of the gizzard+proventriculus, spleen and liver increased with increasing concentrations of SDPP. At 24 d of age, the grain and SDPP inclusion significantly interacted, depressing the weight of bursa and spleen in birds that received the highest concentration of SDPP in the maize-based diet. Birds fed on the maize-based diets had higher relative weight of pancreas than those on the wheat-based diets. Increasing concentrations of SDPP in the starter diet improved the activities of maltase, sucrase and alkaline phosphatase at 24 d of age. The interaction of grain and SDPP concentration was significant for sucrase activity in birds on the wheat-based diets. Chickens on maize-based diets had higher alkaline phosphatase and maltase activities than those on wheat-based diets. Chicks that were offered SDPP-containing starter diets had longer villi, deeper crypts and lower villi/crypt than the control at 24 d of age regardless of the grain type used. Furthermore, longer villi and larger villi/crypt were found in chicken groups fed on wheat-based diets than those on maize-based diets. Chickens on maize-based diets had higher

  14. Effects of diet acidification and xylanase supplementation on performance, nutrient digestibility, duodenal histology and gut microflora of broilers fed wheat based diet

    NARCIS (Netherlands)

    Esmaeilipour, O.; Moravej, H.; Shivazad, M.; Rezaian, M.; Aminzadeh, S.; Krimpen, van M.M.

    2012-01-01

    1. The objective of this experiment was to study the influences of xylanase and citric acid on the performance, nutrient digestibility, digesta viscosity, duodenal histology, and gut microflora of broilers fed on a wheat based diet. 2. The experiment was carried out as a 2 x 3 factorial arrangement

  15. Performance of broiler chickens fed neem ( Azadirachta indica ) leaf ...

    African Journals Online (AJOL)

    One hundred and ninety-two day-old marshal broilers were used in an eight weeks feeding trial to evaluate the effects of neem (Azadirachta indica) leaf meal on growth performance and haematological parameters of broiler chickens. The birds were randomly assigned into four (4) groups of forty eight (48) birds each in a ...

  16. Evaluation of phytate-degrading Lactobacillus culture administration to broiler chickens.

    Science.gov (United States)

    Askelson, Tyler E; Campasino, Ashley; Lee, Jason T; Duong, Tri

    2014-02-01

    Probiotics have been demonstrated to promote growth, stimulate immune responses, and improve food safety of poultry. While widely used, their effectiveness is mixed, and the mechanisms through which they contribute to poultry production are not well understood. Microbial phytases are increasingly supplemented in feed to improve digestibility and reduce antinutritive effects of phytate. The microbial origin of these exogenous enzymes suggests a potentially important mechanism of probiotic functionality. We investigated phytate degradation as a novel probiotic mechanism using recombinant Lactobacillus cultures expressing Bacillus subtilis phytase. B. subtilis phyA was codon optimized for expression in Lactobacillus and cloned into the expression vector pTRK882. The resulting plasmid, pTD003, was transformed into Lactobacillus acidophilus, Lactobacillus gallinarum, and Lactobacillus gasseri. SDS-PAGE revealed a protein in the culture supernatants of Lactobacillus pTD003 transformants with a molecular weight similar to that of the B. subtilis phytase. Expression of B. subtilis phytase increased phytate degradation of L. acidophilus, L. gasseri, and L. gallinarum approximately 4-, 10-, and 18-fold over the background activity of empty-vector transformants, respectively. Phytase-expressing L. gallinarum and L. gasseri were administered to broiler chicks fed a phosphorus-deficient diet. Phytase-expressing L. gasseri improved weight gain of broiler chickens to a level comparable to that for chickens fed a control diet adequate in phosphorus, demonstrating proof of principle that administration of phytate-degrading probiotic cultures can improve performance of livestock animals. This will inform future studies investigating whether probiotic cultures are able to provide both the performance benefits of feed enzymes and the animal health and food safety benefits traditionally associated with probiotics.

  17. Maternally derived anti-fibroblast growth factor 23 antibody as new tool to reduce phosphorus requirement of chicks.

    Science.gov (United States)

    Ren, Zhouzheng; Bütz, Daniel E; Sand, Jordan M; Cook, Mark E

    2017-04-01

    Novel means to reduce phosphate input into poultry feeds and increase its retention would preserve world phosphate reserves and reduce environmental impact of poultry production. Here we show that a maternally derived antibody to a fibroblast growth factor-23 (FGF-23) peptide (GMNPPPYS) alleviated phosphorus deficiency in chicks fed low non-phytate phosphorus (nPP) diets. White Leghorn laying hens were vaccinated with either an adjuvant control or the synthetic FGF-23 peptide, and chicks with control or anti-FGF-23 maternal antibodies were fed a diet containing either 0.13 or 0.45% nPP (experiment 1), and 0.20 or 0.45% nPP (experiment 2) for 14 d. In both experiments, decreasing nPP from 0.45 to 0.13 or 0.20% decreased BW gain, G:F, excreta phosphorus, plasma phosphate, and plasma FGF-23 at all time periods examined (nPP main effect, P posture scores (d 7, 14) and bone lesion scores (d 14) decreased and plasma phosphate (d 14) increased in anti-FGF-23 chicks fed 0.13% nPP, compared to those with control antibody on the same diet (P < 0.05). In experiment 2, chicks with maternal anti-FGF-23 antibody had increased tibiotarsi ash (d 14), and plasma phosphate (d 14) and 1,25(OH)2D3 (d 14) levels, compared to chicks with control antibody (antibody main effect, P < 0.05). BW gain and G:F were increased in chicks with anti-FGF-23 antibody fed 0.20% nPP, compared to control antibody chicks on the same diet, at all time periods examined (P < 0.05). In conclusion, maternally-derived anti-FGF-23 antibody increased phosphorus retention in chicks fed diets containing either 0.13 or 0.20% nPP and thereby, reduced signs of phosphorus deficiency. © 2016 Poultry Science Association Inc.

  18. Immuno-pathological studies on broiler chicken experimentally infected with Escherichia coli and supplemented with neem (Azadirachta indica leaf extract

    Directory of Open Access Journals (Sweden)

    Vikash Sharma

    2016-07-01

    Full Text Available Aim: The present study was conducted to evaluate the effects of neem leaf extract (NLE supplementation on immunological response and pathology of different lymphoid organs in experimentally Escherichia coli challenged broiler chickens. Materials and Methods: For this study, we procured 192-day-old broiler chicks from local hatchery and divided them into Groups A and Group B containing 96 birds each on the first day. Chicks of Group A were supplemented with 10% NLE in water, whereas chicks of Group B were not supplemented with NLE throughout the experiment. At 7th day of age, chicks of Group A were divided into A1 and A2 and Group B into B1 and B2 with 54 and 42 chicks, respectively, and chicks of Groups A1 and B1 were injected with E. coli O78 at 107 colony-forming units/0.5 ml intraperitoneally. Six chicks from each group were sacrificed at 0, 2, 4, 7, 14, 21, and 28 days post infection; blood was collected and thorough post-mortem examination was conducted. Tissue pieces of spleen and bursa of Fabricius were collected in 10% buffered formalin for histopathological examination. Serum was separated for immunological studies. Result: E. coli specific antibody titer was significantly higher in Group A1 in comparison to Group B1. Delayed-type hypersensitivity response against 2,4 dinirochlorobenzene (DNCB antigen was significantly higher in Group A1 as compared to Group B1. Pathological studies revealed that E. coli infection caused depletion of lymphocytes in bursa of Fabricius and spleen. Severity of lesions in Group A1 was significantly lower in comparison to Group B1. Conclusion: 10% NLE supplementation enhanced the humoral as well as cellular immune responses attributed to its immunomodulatory property in experimentally E. coli infected broiler chicken.

  19. Immuno-pathological studies on broiler chicken experimentally infected with Escherichia coli and supplemented with neem (Azadirachta indica) leaf extract.

    Science.gov (United States)

    Sharma, Vikash; Jakhar, K K; Dahiya, Swati

    2016-07-01

    The present study was conducted to evaluate the effects of neem leaf extract (NLE) supplementation on immunological response and pathology of different lymphoid organs in experimentally Escherichia coli challenged broiler chickens. For this study, we procured 192-day-old broiler chicks from local hatchery and divided them into Groups A and Group B containing 96 birds each on the first day. Chicks of Group A were supplemented with 10% NLE in water, whereas chicks of Group B were not supplemented with NLE throughout the experiment. At 7(th) day of age, chicks of Group A were divided into A1 and A2 and Group B into B1 and B2 with 54 and 42 chicks, respectively, and chicks of Groups A1 and B1 were injected with E. coli O78 at 10(7) colony-forming units/0.5 ml intraperitoneally. Six chicks from each group were sacrificed at 0, 2, 4, 7, 14, 21, and 28 days post infection; blood was collected and thorough post-mortem examination was conducted. Tissue pieces of spleen and bursa of Fabricius were collected in 10% buffered formalin for histopathological examination. Serum was separated for immunological studies. E. coli specific antibody titer was significantly higher in Group A1 in comparison to Group B1. Delayed-type hypersensitivity response against 2,4 dinirochlorobenzene (DNCB) antigen was significantly higher in Group A1 as compared to Group B1. Pathological studies revealed that E. coli infection caused depletion of lymphocytes in bursa of Fabricius and spleen. Severity of lesions in Group A1 was significantly lower in comparison to Group B1. 10% NLE supplementation enhanced the humoral as well as cellular immune responses attributed to its immunomodulatory property in experimentally E. coli infected broiler chicken.

  20. Physiological response of heat stressed broiler chickens to ...

    African Journals Online (AJOL)

    Effect of supplementing the drinking water of broilers reared under natural heat stress with ammonium chloride (NH4Cl), sodium bicarbonate (NaHCO3), calcium chloride (CaCl2) and ascorbic acid (AA) on physiological response was investigated. A 200, one-day Arbor acre chicks were randomly allotted to five treatments in ...

  1. Oral antibody to interleukin-10 reduces growth rate depression due to Eimeria spp. infection in broiler chickens.

    Science.gov (United States)

    Sand, Jordan M; Arendt, Maria K; Repasy, Alec; Deniz, Gűlay; Cook, Mark E

    2016-02-01

    Eimeria spp. must be controlled in floor-reared poultry to prevent the onset of coccidiosis. Here we use an oral antibody to chicken IL-10 to prevent growth depression due to Eimeria spp. infection. Egg antibody directed against an antigenic peptide of IL-10 was produced in laying hens and measured using an ELISA. In the first experiment, egg yolk powder containing antibody to chicken IL-10 (vlpramqt conjugate) (anti-IL-10 yolk powder) was fed at 3.4 g/kg feed to determine growth response following mixed Eimeria spp. challenge. Chicks were fed either anti-IL-10 antibodies or control antibodies and challenged (d3) with either sterile saline or a 10× attenuated Eimeria spp. vaccine. Control-fed and Eimeria-challenged chicks grew 8.8% slower than those challenged with saline (P < 0.04), whereas anti-IL-10-fed Eimeria challenged chicks were not different from untreated controls. In the second trial a dose response was performed with doses of either 0 (control antibody), 0.34-, or 3.4-g anti-IL-10 yolk powder/kg feed. Control-fed, Eimeria-challenged chicks grew 10.6% slower than control saline-challenged chicks (P < 0.05); however, anti-IL-10-fed chicks fed either dose of anti-IL-10 were not different from saline-challenged chicks. Finally, the effect of anti-IL-10 on acquired immunity was investigated. Chicks were fed control or anti-IL-10 yolk powder and vaccinated with a 1× dose of Eimeria vaccine at d 3. After 14 d, antibody was removed from the diet. Chicks were either saline or 10× Eimeria challenged at d 17. We found that the anti-IL-10-fed chickens did not show a reduction in growth due to challenge; hence anti-IL-10 does not appear to affect adaptive immunity during the primary immunization. Overall, use of an antibody to IL-10 is a novel method in preventing adverse effects of Eimeria spp. infection in poultry. © 2016 Poultry Science Association Inc.

  2. Effects of dietary onion ( extract supplementation on performance, apparent total tract retention of nutrients, blood profile and meat quality of broiler chicks

    Directory of Open Access Journals (Sweden)

    Siska Aditya

    2017-02-01

    Full Text Available Objective This study was conducted to investigate the effect of dietary onion (Allium cepa extract (OE supplementation on growth performance, apparent total tract retention (ATTR, blood profile, carcass characteristics and meat quality in broilers. Methods Four hundred male broiler chicks (Ross 308, 3-d old were randomly allocated to four dietary treatments for 28 d feeding trial. Each treatment has five replications with 20 birds each. Four dietary treatments were designated according to the OE supplementation levels (0 as control, 5, 7.5, and 10 g of OE per kg of basal diet respectively. On d 28, a total of 20 birds from each treatment were subjected for ATTR, serum biochemical assay, carcass characteristic and organ weight measurement. Results Overall weight gain of OE 7.5 g/kg group was higher (p = 0.04 than control group. The ATTRs of dietary energy (p<0.01 and ether extract (p = 0.04 linearly increased with increasing levels of dietary OE. However, no difference in ATTR of dry matter and crude protein was evident. Furthermore, serum IgG concentration increased linearly (p<0.01 and quadratically (p = 0.03 with increasing OE supplementation. No differences in carcass dressing weight and amount of abdominal fat by treatments were observed. Also, the weight of organ including immune organ was not different among the treatments. The TBARS values of 10 d stored breast meat decreased linearly (p<0.01 and quadratically (p<0.01 with increasing dietary OE levels. The meat color was also affected, with lower (p<0.01 redness score in meats from OE supplemented groups. This study showed that dietary OE improved broiler weight gain presumably by increasing feed intake and ATTR of both energy and ether extract. The dietary OE increased serum IgG level and meat anti-oxidation capacity. Conclusion This study implies that the recommended level of dietary OE supplementation could be beneficial for improving broiler performance and meat quality.

  3. Nigerian Journal of Animal Production - Vol 31 (2004)

    African Journals Online (AJOL)

    Comparative Performance Of Broiler Chicks fed Diets Containing Differently Processed Mucuna pruriens Seed Meals · EMAIL FULL TEXT EMAIL FULL TEXT DOWNLOAD FULL TEXT DOWNLOAD FULL TEXT. OO Emenalom, 12-16 ...

  4. Effects of chicory root powder on growth performance and histomorphometry of jejunum in broiler chicks

    Directory of Open Access Journals (Sweden)

    Homan Izadi

    2013-09-01

    Full Text Available In the present study, chicory root powder (CRP as growth promoter at 1% and 3% levels was supplemented in broilers’ diet to investigate the growth performance and histo- morphometry of jejunum. One hundred twenty, one-day-old male broilers were used in a completely randomized design (CRD with 3 treatments and 4 replicates (10 chicks per replicate. At the end of each period (0-10, 11-24 and 0-24 days, feed intake (FI, weight gain (WG, and feed conversion ratio (FCR were measured. At the end of experiment (day 24, one bird per replicate was sacrificed for breast weight (BW, drumstick weight (DW, and jejunum length (JL as a percentage of body weight, and histomorphometry of villus. The FI increased by 3% CRP in the 1st period (p < 0.01. The percentage of WG significantly increased at 1% during the 1st period and, in the 2nd and total periods, it increased only at 3% CRP (p < 0.05. The FCR decreased at 1% in the 1st (p < 0.04 and, at 3% in the 2nd (p < 0.01 and total periods (p < 0.05. The percentage of DW increased at 3% CRP (p < 0.05. The treatments increased the percentage of BW (p < 0.059 and, percentage of JL (p < 0.079 as well. The villus width and, crypt depth (CD at 1% and 3% CRP and, villus surface at 3% reduced. The 3% CRP increased the villus length (VL and villi number (p < 0.05 and, VL/CD (p < 0.01 and, villus surface area (p < 0.02. The percentage of leaf-like villi decreased in CRP treatments (p < 0.05. The number of goblet cells increased in CRP treatments (p < 0.01. In conclusion, chicory root powder can improve growth performance in broilers by enhancing food digestion and absorption through modification of jejunum histomorphometry.

  5. Influence of sex, age, and fasting on blood parameters and body, bursa, spleen and yolk sac weights of broiler chicks

    Directory of Open Access Journals (Sweden)

    DL Pires

    2007-12-01

    Full Text Available The effects of water and feed fasting for 24, 48 and 72 hours post-hatching on blood parameters (mean corpuscular volume, MCV; red blood-cell, RBC; hematocrit, HCT; hemoglobin, HGB; plasma glucose, CGP; plasma total protein, PP, and differential leukocytes count, and on body, liver, spleen, bursa, and yolk sac weights were analyzed. Erythrogram data were obtained with a blood cell counter. Total plasma protein and plasma glucose were determined by using the Bradford method (1976 and a glucose PAP liquiform kit (Labtest, cat. n. 84, respectively. Specific leukocyte counts were carried out on blood smears stained with Rosenfeld solution. According to the obtained data, water and feed post-hatching fasting reduced MCV values, which also were lower in males than that in females. Fasting for 48 hours promoted an increase in PP, while fasting for 72 hours reduced HCT. Chicks submitted to fasting presented lower body weights as compared to fed chicks, but their liver weight did not increase between 48 and 72 hours of age. Fasting decreased spleen weight, but bursa and yolk sac weight were not affected. Data showed that female and male chicks react in a similar way to post-hatching fasting, which affects body weight, liver and spleen weight, and HCT and PP values. Moreover, 72 hours of fasting affected more intensely HCT and MCV values.

  6. Effect of protein, carbohydrate, lipid, and selenium levels on the performance, carcass yield, and blood changes in broilers

    Directory of Open Access Journals (Sweden)

    FH Hada

    2013-12-01

    Full Text Available The objective of this study was to evaluate the performance, carcass and parts yield, and blood changes in broilers fed different protein, carbohydrate, and lipid levels. Birds were fed a commercial diet until seven days of age. On day 8, birds were distributed according to a completely randomized experimental design in a 4 x 2 factorial arrangement (control diet, low protein diet, low carbohydrate diet or low lipid diet vs. supplementation of 0 or 0.3ppm organic selenium with four replicates of 15 birds each. Broilers fed low protein presented lower body weight, feed intake, and worse feed conversion ratio on day 42, as well as lower carcass and breast yields, higher leg and abdominal fat yields, higher triglyceride and lower uric acid blood levels. Broilers fed the low carbohydrate diets presented low glucose levels on days 14 and 42.Creatine-kinase (CK levels increased as birds aged. The livability of broilers fed the low protein diets improved and of those fed low carbohydrate diets worsened with dietary selenium addition on days 35 and 42. Selenium supplementation increased glucose levels in 42-d-old broilers. Changes in dietary protein caused more impact on broiler performance compared with carbohydrates and lipids. Changes in macronutrients caused metabolic changes in broilers. Selenium affected broiler livability as measured on days 35 and 42, and glucose blood levels.

  7. Impact of transportation duration on stress responses in day-old chicks from young and old breeders.

    Science.gov (United States)

    Jacobs, Leonie; Delezie, Evelyne; Duchateau, Luc; Goethals, Klara; Ampe, Bart; Buyse, Johan; Tuyttens, Frank Andre Maurice

    2017-06-01

    The aim of this study was to assess the interaction effect of transportation duration and parental age on physiological stress responses and quality in day-old chicks. After hatch, 3240 chicks from either 29-week old (young) or 60-week old (old) broiler breeders were transported for 1.5h (short) or 11h (long). Thereafter, 228 chicks were assessed for quality and blood plasma was assayed for corticosterone (CORT), lactate, glucose, and thiobarbituric acid-reactive substances (TBARS, increased concentrations can indicate oxidative stress). No interaction effects of parental age and transportation duration were found (all P>0.10). Chicks from young breeders showed higher CORT levels (P=0.007) and were of higher quality (Tona method, Ptransportation, chicks showed increased CORT (Ptransportation. No evidence was provided that long transportation differently affected the quality or stress responses of chicks from breeder flocks of two ages. Copyright © 2017. Published by Elsevier Ltd.

  8. Effect of inulin supplementation and dietary fat source on performance, blood serum metabolites, liver lipids, abdominal fat deposition, and tissue fatty acid composition in broiler chickens.

    Science.gov (United States)

    Velasco, S; Ortiz, L T; Alzueta, C; Rebolé, A; Treviño, J; Rodríguez, M L

    2010-08-01

    A study was conducted to evaluate the effect of adding inulin to diets containing 2 different types of fat as energy sources on performance, blood serum metabolites, liver lipids, and fatty acids of abdominal adipose tissue and breast and thigh meat. A total of 240 one-day-old female broiler chicks were randomly allocated into 1 of 6 treatments with 8 replicates per treatment and 5 chicks per pen. The experiment consisted of a 3 x 2 factorial arrangement of treatments including 3 concentrations of inulin (0, 5, and 10 g/kg of diet) and 2 types of fat [palm oil (PO) and sunflower oil (SO)] at an inclusion rate of 90 g/kg of diet. The experimental period lasted from 1 to 34 d. Dietary fat type did not affect BW gain but impaired feed conversion (P abdominal fat deposition and serum lipid and glucose concentrations. Triacylglycerol contents in liver were higher in the birds fed PO diets. Dietary fat type also modified fatty acids of abdominal and i.m. fat, resulting in a higher concentration of C16:0 and C18:1n-9 and a lower concentration of C18:2n-6 in the birds fed PO diets. The addition of inulin to diets modified (P = 0.017) BW gain quadratically without affecting feed conversion. Dietary inulin decreased the total lipid concentration in liver (P = 0.003) and that of triacylglycerols and very low density lipoprotein cholesterol (up to 31%) in blood serum compared with the control groups. The polyunsaturated fatty acid:saturated fatty acid ratio increased in abdominal and i.m. fat when inulin was included in the SO-containing diets. The results from the current study suggest that the addition of inulin to broiler diets has a beneficial effect on blood serum lipids by decreasing triacylglyceride concentrations The results also support the use of inulin to increase the capacity of SO for enhancing polyunsaturated fatty acid:saturated fatty acid ratio of i.m. fat in broilers.

  9. Performance Of Growing Pigs And Finisher Broilers Housed Together

    African Journals Online (AJOL)

    Growth performance and cost of feeding young growing pigs and finisher broilers under integrated broiler/pig production system were investigated. Four young growing pigs (the control) were housed in pen A and fed 4% of their body weight as commercial growers feed. Another 4 were housed in pen B with broilers in ...

  10. Growth Performance, Carcass Characteristics, Antibody Titer and Blood Parameters in Broiler Chickens Fed Dietary Myrtle (Myrtus communis Essential Oil as an Alternative to Antibiotic Growth Promoter

    Directory of Open Access Journals (Sweden)

    Mahmoodi Bardzardi M

    2014-03-01

    Full Text Available This experiment was conducted to determine the effects of Myrtle Essential Oil (MEO on growth performance, carcass characteristics, antibody titer and blood parameters of broiler chickens. A total of 200 Ross 308 broiler chickens were allocated to five dietary treatments with four replicates of 10 birds each. Dietary treatments were prepared by formulating a corn-soybean meal-based diet free of antibiotics (Control and supplementing the basal diet with three levels of MEO at 100, 200, 300 mg/Kg or antibiotic Flavophospholipol (FPL at 600 mg/Kg. The results showed that diets supplemented with MEO and FPL increased the feed intake, body weight gain and improved the feed conversion ratio compared to the control treatment (P. The relative carcass weight was significantly increased, whereas the weight of gastrointestinal tract and liver were decreased in broilers fed MEO (P. Supplementing the basal diet with MEO increased the antibody titers against Avian Influenza Virus (AIV and Newcastle disease Virus (NDV, although supplementing diet with 200 mg/Kg of MEO was more effective (P. Broilers fed MEO diets especially at the level of 300 mg/Kg had a lower white blood cells count and heterophil, heterophil to lymphocyte ratio, mean corpuscular volume and mean corpuscular hemoglobin, but a higher lymphocyte and red blood cells count (P. In conclusion, data showed that diet supplemented with MEO improved the growth performance and increased antibody titers against AIV and NDV, especially at the level of 200 mg/Kg, in broiler chickens and could be an adequate alternative to antibiotics.

  11. The effects of thermal manipulations during embryogenesis of broiler chicks on growth of embryo and skeletal traits

    Energy Technology Data Exchange (ETDEWEB)

    Aygün, Ali, E-mail: aaygun@selcuk.edu.tr [Selcuk University, Faculty of Agriculture, Department of Animal Science, Konya, 42075 (Turkey); Narinç, Doğan, E-mail: narincd@gmail.com [Namik Kemal University, Faculty of Veterinary Medicine, Department of Genetics, Tekirdag, 59100 (Turkey)

    2016-04-18

    Incubation temperature is one of the important environmental factors that can induce epigenetic thermal adaptation of different physiological control systems. Thus, post hatch thermo tolerance ability of birds may be gained using these manipulations during different incubation periods. The current study was carried out to reveal the effects of temperature manipulations during early and late embryogenesis on weight of embryo and size of skeletal bilateral traits (face, wings, metatarsus, tibia, and femur) in broiler chicken embryos. One thousand commercial broiler eggs from 46 week old breeder flock were used in study. Treatments consisted of eggs incubated at 37.8°C and 55% relative humidity throughout (control; DG1), heated to 36.9°C and supplied 60% relative humidity for 6 hours daily from day 0 to 8 (DG2), heated to 36.9°C and supplied 60% relative humidity for 6 hours daily from day 10 to 18 (DG3), heated to 41°C and supplied 65% relative humidity for 3 hours daily from day 8 to 10 (DG4), and heated to 41°C and supplied 65% relative humidity for 3 hours daily from day 16 to 18 (DG5). Measurements of embryo weight and bilateral traits were obtained at 20 day of incubation and at hatch (at day 21). It was determined that the live weights of embryo and chick were affected significantly by treatment; DG3 group has shown higher mean values than the other treatment groups (P<0.05). There were differences in lengths of femur, tibia and metatarsus among treatment groups at hatch. Particularly, the high incubator temperatures at the second half of incubation accelerated growth of body and bone in embryos. These consequences of the treatments performed at different temperatures and times indicate that the different metabolic shifts realized by the embryos.

  12. Standardized Ileal Amino Acid Digestibility of Commonly Used Feed Ingredients in Growing Broilers

    Directory of Open Access Journals (Sweden)

    Zafar Ullah

    2016-09-01

    Full Text Available This experiment was conducted to determine standardized ileal amino acid digestibility (SIAAD of commonly used feed ingredients in poultry diets in Pakistan. These feed ingredients included corn, rice broken (RB, rice polishings (RP, wheat bran (WB, sunflower meal (SFM, cottonseed meal (CSM, guar meal (GM, soybean meal (SBM from India and Argentine and fish meal (FM. The SIAAD of each ingredient was determined in triplicate using 21-days-old broilers. Day-old male broiler chicks (Hubbard× Hubbard were reared on corn-SBM based diet from 1 to 13 days and thereafter birds were fed experimental diets from day 14 to 21. Each diet was fed to 36 birds kept in six replicate cages, each cage had six birds. In cereals, the SIAAD of corn’s amino acid (AA (90.1% was similar (p>0.05 to RB (89.0%. Isoleucine (97.8% and lysine (96.9% were highly digestible AA in corn and RB, respectively. Among cereal-by products, WB’s SIAAD (76.9% was same (p>0.05 as RP (71.9%. Arginine from WB (82.5% and RP (83.2% was highly digestible. However, threonine in WB (72.7% and leucine in RP (69.6% were the lowest digestible AAs. In plant protein meals, AAs from Argentine-SBM (85.1% and Indian-SBM (83.4% had higher (p0.05. The SBM from plant protein meals had higher (p<0.05 SIAAD than other studied feed ingredients. However, the GM had the lowest (p<0.05 SIAAD among protein meals.

  13. Optimization of phase feeding of starter, grower, and finisher diets for male broilers by mixture experimental design: forty-eight-day production period.

    Science.gov (United States)

    Roush, W B; Boykin, D; Branton, S L

    2004-08-01

    A mixture experiment, a variant of response surface methodology, was designed to determine the proportion of time to feed broiler starter (23% protein), grower (20% protein), and finisher (18% protein) diets to optimize production and processing variables based on a total production time of 48 d. Mixture designs are useful for proportion problems where the components of the experiment (i.e., length of time the diets were fed) add up to a unity (48 d). The experiment was conducted with day-old male Ross x Ross broiler chicks. The birds were placed 50 birds per pen in each of 60 pens. The experimental design was a 10-point augmented simplex-centroid (ASC) design with 6 replicates of each point. Each design point represented the portion(s) of the 48 d that each of the diets was fed. Formulation of the diets was based on NRC standards. At 49 d, each pen of birds was evaluated for production data including BW, feed conversion, and cost of feed consumed. Then, 6 birds were randomly selected from each pen for processing data. Processing variables included live weight, hot carcass weight, dressing percentage, fat pad percentage, and breast yield (pectoralis major and pectoralis minor weights). Production and processing data were fit to simplex regression models. Model terms determined not to be significant (P > 0.05) were removed. The models were found to be statistically adequate for analysis of the response surfaces. A compromise solution was calculated based on optimal constraints designated for the production and processing data. The results indicated that broilers fed a starter and finisher diet for 30 and 18 d, respectively, would meet the production and processing constraints. Trace plots showed that the production and processing variables were not very sensitive to the grower diet.

  14. Growth performance and immunological responses of broiler ...

    African Journals Online (AJOL)

    This study was conducted to determine the growth performance and immune response of broiler chickens fed synbiotic and diet acidifier to Newcastle disease vaccinations. One hundred and forty four (144) day old broiler chickens were randomly assigned to four dietary treatments replicated thrice with 12 birds per replicate ...

  15. Effects of feed contaminant deoxynivalenol on plasma cytokines and mRNA expression of immune genes in the intestine of broiler chickens.

    Science.gov (United States)

    Ghareeb, Khaled; Awad, Wageha A; Soodoi, Chimidtseren; Sasgary, Soleman; Strasser, Alois; Böhm, Josef

    2013-01-01

    An experiment was conducted to investigate the individual and combined effects of dietary deoxynivalenol (DON) and a microbial feed additive on plasma cytokine level and on the expression of immune relevant genes in jejunal tissues of broilers. A total of 40 broiler chicks were obtained from a commercial hatchery and divided randomly into four groups (10 birds per group). Birds were reared in battery cages from one day old for 5 weeks. The dietary groups were 1) control birds fed basal diet; 2) DON group fed basal diet contaminated with 10 mg DON/ kg feed; 3) DON + Mycofix group fed basal diet contaminated with 10 mg DON/ kg feed and supplemented with a commercial feed additive, Mycofix® Select (MS) (2.5 kg/ton of feed); 4) Mycofix group fed basal diet supplemented with MS (2.5 kg/ton of feed). At 35 days, the plasma levels of tumor necrosis factor alpha (TNF-α) and interleukin 8 (IL-8) were quantified by ELISA test kits. Furthermore, the mRNA expression of TNF-α, IL-8, IL-1β, interferon gamma (IFNγ), transforming growth factor beta receptor I (TGFBR1) and nuclear factor kappa-light-chain-enhancer of activated B cells 1 (NF-κβ1) in jejunum were quantified by qRT-PCR. The results showed that the plasma TNF-α decreased in response to DON, while in combination with MS, the effect of DON was reduced. DON down-regulated the relative gene expression of IL-1β, TGFBR1 and IFN-γ, and addition of MS to the DON contaminated diet compensates these effects on IL-1β, TGFBR1 but not for IFN-γ. Furthermore, supplementation of MS to either DON contaminated or control diet up-regulated the mRNA expression of NF-κβ1. In conclusion, DON has the potential to provoke and modulate immunological reactions of broilers and subsequently could increase their susceptibility to disease. The additive seemed to have almost as much of an effect as DON, albeit on different genes.

  16. Haematological and Serum Biochemical Parameters of Broiler Chickens Fed Varying Dietary Levels of Fermented Castor Oil Seed Meal (Ricinus communis L. and Different Methionine Sources in South Western Nigeria

    Directory of Open Access Journals (Sweden)

    Ayorinde David Adeniran

    2017-09-01

    Full Text Available In this experiment, the effect of varying dietary levels of fermented castor oil seed meal (FCSM and different methionine sources (DL-methionine and herbal methionine on haematological and serum biochemical parameters of broilers. A total of 240 one-day-old Anak broiler chicks were used in the experiment lasted 56 days. The dietary experiment was laid out as a completely randomized design in a 4 × 2 factorial arrangement consisting of 4 dietary levels of FCSM (0, 50, 100 and 150 g/kg and 2 methionine sources (DL-methionine and herbal methionine. The birds were weighed and randomly distributed into 8 treatments with 3 replicates of 10 birds each. During the starter phase of the experiment, haemoglobin, red blood cell count, mean corpuscular haemoglobin concentration and eosinophil counts were higher (P

  17. Correlation between serum lipoproteins and abdominal fat pad in ...

    African Journals Online (AJOL)

    In this experiment, four hundred day-old broiler chicks were assigned randomly to 16 floor pens with 25 chicks each. Three diets containing 0% fish oil + 7% soybean oil, 3.5% fish oil + 3.5% soybean oil and 7% fish oil + 0% soybean oil and a free oil control diet were formulated. The birds fed fish oil diet showed a lower ...

  18. Growth performance, carcass traits and meat quality of yellow-feathered broilers fed graded levels of alfalfa meal with or without wheat.

    Science.gov (United States)

    Jiang, Shouqun; Gou, Zhongyong; Li, Long; Lin, Xiajing; Jiang, Zongyong

    2018-03-01

    The effects of 0, 40 and 80 g/kg alfalfa meal on growth performance, carcass traits and meat quality of Chinese yellow-feathered broilers fed diets containing or lacking wheat (0 or 200 g/kg) as part of the energy source, were examined using random design with a 2 × 3 factorial arrangement of treatments. Dressing percentage and semi-eviscerated proportion were lower, and meat color a* (redness) value was higher in birds fed diets containing wheat than diets lacking wheat (P meat was higher in chickens fed corn-based diets than in those fed wheat (P Meat from those supplemented with 40 g/kg alfalfa meal had better taste than the other two levels (P meat color and lower drip loss than those fed the diets without wheat, and adding 40 g/kg alfalfa meal generally improved meat quality and taste. © 2017 Japanese Society of Animal Science.

  19. Effects of dietary conjugated linoleic acid (CLA), n-3 and n-6 fatty ...

    African Journals Online (AJOL)

    An experiment was conducted on broiler chickens to study the effects of conjugated linoleic acid (CLA), fish oil, soybean oil or their mixtures (at 7% for single and 3.5% + 3.5% for mixtures) as well as up 12% dosage of palm oil, on the performance and carcass traits of broiler chickens. The chicks fed 7% fish oil or 7% CLA ...

  20. Influence of postbiotic RG14 and inulin combination on cecal microbiota, organic acid concentration, and cytokine expression in broiler chickens.

    Science.gov (United States)

    Kareem, K Y; Loh, T C; Foo, H L; Asmara, S A; Akit, H

    2017-04-01

    This study examined the effects of different combinations of inulin and postbiotics RG14 on growth performance, cecal microbiota, volatile fatty acids (VFA), and ileal cytokine expression in broiler chickens. Two-hundred-and sixteen, one-day-old chicks were allocated into 6 treatment groups, namely, a basal diet (negative control, NC), basal diet + neomycin and oxytetracycline (positive control, PC), T1 = basal diet + 0.15% postbiotic RG14 + 1.0% inulin, T2 = basal diet + 0.3% postbiotic RG14 + 1.0% inulin, T3 = basal diet + 0.45% postbiotic RG14 + 1.0% inulin, and T4 = basal diet + 0.6% postbiotic RG14 + 1.0% inulin, and fed for 6 weeks. The results showed that birds fed T1 and T3 diets had higher (P  0.05) among diets. The NC birds had higher (P inulin combinations are potential replacements for antibiotic growth promoters in the poultry industry. © 2016 Poultry Science Association Inc.

  1. Isotopic traceability in body tissues and in different breeding periods of broilers

    Directory of Open Access Journals (Sweden)

    R.P. Oliveira

    2012-10-01

    Full Text Available The inclusion of poultry viscera meal (VM in broiler diets to possibly replace it with strictly vegetable diet (VE and vice-versa, using the technique of carbon and nitrogen stable isotopes in different tissues was traced. One hundred ninety-two one-day-old chicks that had been distributed randomly were used in twelve experimental treatments with four replications of four chicks each. The treatments consisted of vegetable diet (VG for diets containing FV going after a certain age or the reverse, in which the birds began feeding VM on diet and then switched to VE diet. At 42 days of age, samples of pectoral muscle (Pectoralis major, keel and tibia were collected, with later determination of isotopic ratios (13C/12C and 15N/14N. The stable isotopes technique couldn't trace the use of VM in feeding broilers when this ingredient was part of broiler diets only in the first seven, 14 or 21 days of age. However, this technique can be applied to younger poultry that have been sampled before a possible change in diet, since they can have the isotopic signature of feeding stabilized around two weeks of age.

  2. The effect of low doses of gamma rays on hatchability of broiler parent stocks

    International Nuclear Information System (INIS)

    Zakaria, Abdul-Hamid

    1990-01-01

    It is a summary of the report written about the three experiments which have been carried out to study the stimulatory effect of different doses of gamma irradiation on the hatchability of broiler parent stocks at 45 weeks of age. It has been shown that doses at 0.1 and 0.15 GY had a stimulatory effect on hatchability of highly-fertile broiler parent stock. Doses at 1.20, 1.60 and 2.10 GY had an inhibitory effect on hatchability of low-fertile broiler parent stock. No significant difference has been detected on the chicks weights hatched from eggs exposed to doses ranged from 0.05 to 1.20 GY of highly-fertile broiler parent stock. 4 tabs

  3. Dietary oxidized poultry offal fat: broiler performance and oxidative stability of thigh meat during chilled storage

    Directory of Open Access Journals (Sweden)

    AMC Racanicci

    2008-03-01

    Full Text Available Two experiments were conducted to evaluate the effects of dietary oxidized poultry offal fat on the performance of broilers and on the oxidative stability of dark chicken meat. One hundred and sixty male chicks were fed a corn-soybean meal diet containing 4% fresh or oxidized poultry fat from 10 to 47 days of age. Fresh fat was stored frozen until diets were produced, and oxidized fat was obtained by electrical heating (110 to 120 ºC. Birds were slaughtered at 47 days of age, and carcass characteristics were measured. Skinless and deboned thigh meat was stored chilled during 12 days, and samples were periodically collected to assess their quality and oxidative stability. Dietary oxidized fat did not affect bird performance or carcass characteristics. During chilled storage, meat color (L*, a* and b* was not affected by dietary treatments; however, TBARS (Thiobarbituric Acid Reactive Substances values were higher (P<0.05 in thigh meat from chickens fed the oxidized fat, indicating that oxidative stability was adversely affected.

  4. Bacillus amyloliquefaciens supplementation alleviates immunological stress in lipopolysaccharide-challenged broilers at early age.

    Science.gov (United States)

    Li, Y; Zhang, H; Chen, Y P; Yang, M X; Zhang, L L; Lu, Z X; Zhou, Y M; Wang, T

    2015-07-01

    This study was conducted to investigate the effect of Bacillus amyloliquefaciens ( BA: ) on the immune function of broilers challenged with lipopolysaccharide ( LPS: ). 192 one-day-old male Arbor Acre broiler chickens were randomly distributed into four treatments: 1) broilers fed a basal diet; 2) broilers fed a basal diet supplemented with BA; 3) LPS-challenged broilers fed a basal diet; and 4) LPS-challenged broilers fed a basal diet supplemented with BA. Each treatment consisted of six replicates with eight broilers per replicate. Broilers were intraperitoneally injected with either 500 μg LPS per kg body weight or sterile saline at 16, 18 and 20 d of age. LPS decreased the average daily gain ( ADG: , P = 0.001) and average daily feed intake (P = 0.001). The decreased ADG (P = 0.009) and increased feed conversion ratio (P = 0.047) in LPS-challenged broilers were alleviated by BA. LPS increased the relative spleen weight (P = 0.001). Relative spleen (P = 0.014) and bursa (P = 0.024) weights in the LPS-challenged broilers were reduced by BA. LPS increased white blood cell ( WBC: ) numbers (P = 0.001). However, the WBC numbers (P = 0.042) and the ratio of lymphocytes to WBC (P = 0.020) in LPS-challenged broilers were decreased with BA treatment. LPS decreased plasma lysozyme activity (P = 0.001), but increased concentrations of plasma corticosterone (P = 0.012) and IL-2 (P = 0.020). In contrast, BA increased lysozyme activity in plasma (P = 0.040). LPS increased mRNA abundances of splenic toll-like receptor 4 (P = 0.046), interferon γ (P = 0.008), IL-1β (P = 0.045) and IL-6, (P = 0.006). IL-2 (P = 0.014) and IL-6 (P = 0.074) mRNA abundances in LPS-challenged broilers were reduced by BA, although BA had an opposite effect for IL-10 mRNA expression in those broilers (P = 0.004). In conclusion, BA supplementation could partially alleviate the compromised growth performance and immune status of broilers under immune stress induced by LPS challenge at early age.

  5. Effect of litter moisture on the development of footpad dermatitis in broiler chickens.

    Science.gov (United States)

    Taira, Kazuyo; Nagai, Toshimune; Obi, Takeshi; Takase, Kozo

    2014-04-01

    Broiler chicks were reared on either wet litter or dry litter to compare the development of footpad dermatitis (FPD). Broilers reared on wet litter first developed FPD at 14 days of age. Their FPD scores increased sharply after 21 days of age, reaching 2.92 at 42 days. In broilers reared on dry litter, FPD was first observed at 28 days of age, and the FPD score was only 0.70 at 42 days. When 21- or 28-day-old broilers that had been reared on wet litter and had developed FPD were moved to dry litter, the progression of FPD was suppressed or delayed. These results suggest that reducing litter moisture is effective in preventing FPD and suppressing disease progression.

  6. Performance of finisher broiler chickens as affected by different ...

    African Journals Online (AJOL)

    A total of 160 male broiler chicks, 21days old and weighing 416.32g on average, were randomly distributed into 32 experimental units of 4 birds each. ... Parameters measured were feed consumption, live weight and weight gain, feed conversion ratio, feeding cost for the production of one kg live weight, carcass yields, ...

  7. Effect of exogenous xylanase, amylase, and protease as single or combined activities on nutrient digestibility and growth performance of broilers fed corn/soy diets.

    Science.gov (United States)

    Amerah, A M; Romero, L F; Awati, A; Ravindran, V

    2017-04-01

    Two trials (a 42-d performance and a 21-d cohort digestibility) were conducted to evaluate the performance and nutrient digestibility of broilers fed corn diets supplemented with exogenous xylanase, amylase, and protease as single or combined activities. A nutritionally adequate, positive control (PC) diet was formulated. The negative control (NC) diet was formulated to be lower in metabolizable energy (∼86 kcal/kg diet) and digestible amino acids (1 to 2%) compared to PC. The other 4 treatments were based on the NC and they were either supplemented with xylanase (X), amylase (A), protease (P), or a combination of X, A, and P (XAP; to provide 2,000 U of X, 200 U of A, and 4,000 U of P/kg diet). All diets were marginal in AvP and Ca and contained a background of phytase (1,000 FTU/kg). In each trial, male broiler (Ross 308) chicks were allocated to the 5 treatments (10 replicates of 20 birds/pen and 9 replicates of 8 birds/cage for the performance and digestibility trials, respectively). In the digestibility trial, ileal digesta was collected on d21 for the determination of nutrient utilization. Data were subjected to one-way ANOVA and means were separated by Tukey's HSD test. Only the XAP improved (P digestibility and apparent ileal digestible energy (AIDE). Both P and XAP improved N retention. The relative improvement in energy digestibility due to enzyme supplementation was greater at the ileal level than that measured in the excreta. The measured changes on AIDE due to supplemental enzymes were much higher than the sum of calculated contributions from starch, fat, and protein. Supplementation of all enzymes reduced (P  0.05) by dietary treatments. Both X and XAP had lower (P  0.05) FCR compared to PC. In conclusion, these results suggest a synergistic effect between X, A and P on broiler performance and nutrient digestibility. In the current study, AIDE measurements appeared to overestimate the enzyme response. Calculation of the energy contribution by

  8. Xanthophyll supplementation reduced inflammatory mediators and apoptosis in hens and chicks.

    Science.gov (United States)

    Gao, Y-Y; Jin, L; Ji, J; Sun, B-L; Xu, L-H; Wang, Q-X; Wang, C-K; Bi, Y-Z

    2016-05-01

    This study investigated effects of xanthophylls (containing 40% lutein and 60% zeaxanthin) on gene expression of inflammatory mediators ( [] and []) and apoptosis ( [] and ) of breeding hens and chicks. In Exp. 1, 432 hens were divided into 3 groups and fed diets supplemented with 0 (as the control group), 20, or 40 mg/kg xanthophylls. The liver, duodenum, jejunum, and ileum were sampled after 35 d. Results showed that 40 mg/kg of xanthophyll addition decreased in the liver, in the liver and duodenum, and in the liver and jejunum while increasing level in the liver and jejunum. Experiment 2 was a 2 × 2 factorial design. Male chicks hatched from hens fed 0 or 40 mg/kg xanthophyll diets were fed diets containing either 0 or 40 mg/kg xanthophylls. The liver, duodenum, jejunum, and ileum were sampled at 0, 7, 14, and 21 d after hatching. Results showed that in ovo xanthophylls reduced inflammatory mediators and apoptosis in the liver, duodenum, and jejunum of chicks mainly within 1 wk after hatching, whereas dietary xanthophylls only decreased expression in the liver from 2 wk onward. These results underlined important anti-inflammatory and antiapoptotic effects of maternal but not progeny dietary xanthophylls. In conclusion, xanthophylls can suppress inflammatory mediators and apoptosis in different tissues of hens and chicks.

  9. Metabolism of serine in growing rats and chicks at various dietary protein levels

    International Nuclear Information System (INIS)

    Tanaka, Hideyuki; Yamaguchi, Michio; Kametaka, Masao

    1976-01-01

    The metabolic fate of the carbon skeleton of L-serine-U- 14 C has been investigated, in vivo and in vitro, in growing rats and chicks fed the diets with various protein calories percents (C %) at 410 kcal of metabolizable energy. The incorporation of 14 C into body protein at 12 hr after the injection of serine- 14 C was about 49% of the injected dose in rats fed the 10 or 15 PC% diet, though the value was reduced in rats fed lower and higher protein diets. The 14 CO 2 production was smaller in rats fed the 10 and 15 PC% diet, and it showed an inverse pattern to that of the 14 C incorporation into body protein. Urinary excretion of 14 C was higher in rats fed 10 and higher PC% diets, whose growth rate and net body protein retention were maximum. In contrast to the case of rats, the incorporation of 14 C into body protein of chicks at 6 hr after the injection was rather reduced in the 15 PC% group. The proportion of 14 C excreted as uric acid was remarkably increased above the 10 PC% group, and about 19% of the injected dose was recovered in the 50 PC% group. The catabolic rate of serine in the liver slices of rats and chicks was increased by high protein diets. These results support the concept that the nutritional significance of metabolism of the carbon skeleton of serine in growing rats and chicks is different from each other, especially at high protein diets. (auth.)

  10. Supplementation of Morinda citrifolia waste as bioactive compound on the performances of broiler

    Directory of Open Access Journals (Sweden)

    I.A.K Bintang

    2007-03-01

    Full Text Available A study on the use of dried Morinda citrifolia waste as feed additive in broiler ration was conducted. One hundred day old chicks (doc were allocated into 4 levels (control; 0.0; 1.2; 2.4 and 4.8 g/kg feed of M. citrifolia waste with 5 replication. Each replication had 5 birds. The treatments were allocated in a completely randomized design. Variables measured were: feed intake, live weight, feed conversion ratio (FCR, percentages of carcass and internal organs ( liver. gizard, abdominal fat, and thickness of intestine. The results showed that M citrifolia waste supply did not significantly (P>0.05 affect all variables measured, but feed intake of those fed with high levels (4,8 g/kg of M. citrifolia waste was significantly (P<0.05 lower than those fed with 1.2 g/kg. FCR was significantly (P<0.05 lower as compared to the control; 1.2 and 2.4 g/kg. It is concluded that the best treatment was the supplementation of M. citrifolia waste of 4.8 g/kg, this treatment improved feed efficiency by 5% as compared to the control.

  11. Dietary restriction causes chronic elevation of corticosterone and enhances stress response in red-legged kittiwake chicks

    Science.gov (United States)

    Kitaysky, A.S.; Kitaiskaia, E.V.; Wingfield, J.C.; Piatt, John F.

    2001-01-01

    Release of corticosterone in hungry kittiwake chicks facilitates begging and allows them to restore depleted energy reserves by increasing parental food provisioning. However, in order to avoid detrimental effects of chronic elevation of corticosterone, chicks might suppress adrenocortical activity in response to prolonged food shortages. In this study we examined temporal dynamics of corticosterone release in red-legged kittiwake (Rissa brevirostris) chicks exposed to prolonged restrictions in energy content and/or nutritional quality (low versus high lipid content) of their food. Starting at the age of 15 days, chicks were fed either high- or low-lipid fish at 40%, 65%, and 100% of ad libitum energy intake. Body mass measurements and baseline plasma samples were taken on a weekly basis after beginning of the treatment. After 3 weeks of treatment, chicks were exposed to a standardized acute handling and restraint stress protocol, where in addition to a baseline sample, three plasma samples were taken at intervals up to 50 min. We found that food-restricted chicks had lower body mass, chronically (during 2-3 weeks) elevated baseline and higher acute stress-induced levels of corticosterone compared to chicks fed ad libitum. Low lipid content of food further exacerbated these effects. An increase in baseline levels of corticosterone was observed within a week after energy requirements of food-restricted chicks exceeded their daily energy intake. A tendency for suppression of adrenocortical activity was observed in treatments fed low-lipid diets only at the end of the experiment. We suggest that nest-bound chicks, if food-stressed, might suffer deleterious effects of chronic elevation of corticosterone.

  12. Antigenotoxic Effect of Piperine in Broiler Chickens Intoxicated with Aflatoxin B1.

    Science.gov (United States)

    da Silva Cardoso, Verônica; Vermelho, Alane Beatriz; Ribeiro de Lima, Cristina Amorim; Mendes de Oliveira, Jéssica; Freire de Lima, Marco Edilson; Pinto da Silva, Lúcia Helena; Direito, Glória Maria; Miranda Danelli, Maria das Graças

    2016-10-31

    Piperine is an abundant amide extracted from black pepper seeds which has been shown to have protective effects against cytotoxic and genotoxic carcinogenesis induced by certain chemical carcinogens and aflatoxin B₁ (AFB₁) in vitro. The aim of this work was to study, in vivo, the antigenotoxic potential of feed-added piperine on broiler chickens experimentally intoxicated with AFB₁, using micronucleus and comet assays. The antigenotoxicity assessment of 9-day-old chicks was performed on a total of 60 chickens divided into four groups of 15 broilers each: (C) control, (P) 60 mg·piperine kg -1 feed, (A) 0.5 mg·AFB₁·kg -1 body weight, (daily by oral route), and (P + A) co-treatment with piperine and AFB₁. The experiment was conducted for 26 days. Chicks intoxicated with AFB₁ showed significant genotoxic effects in the first 24 h post intoxication, and the effects remained in the other periods analyzed (48, 72, and 96 h and 26 days of treatment). The DNA damage in peripheral blood cells, the number of erythrocytes with micronuclei, and polychromatic-to-normochromatic erythrocyte ratio were significantly reduced or absent in the piperine/AFB₁ group. No significant differences were observed between the group piperine/AFB₁ and the control and piperine-alone groups. The addition 60 mg·kg -1 of piperine to the diet of the broiler chicks was safe, promoting beneficial effects in poultry health with respect to the toxic effects 0.5 mg·AFB₁·kg -1 body weight.

  13. Performance of starting broiler chicks on sandbox ( Hura crepitans ...

    African Journals Online (AJOL)

    A 21-day feeding trial was conducted to evaluate the feeding value of raw and cooked Sand box (Hura crepitans) seed meal for broiler chickens . Sand box seeds (100kg) were divided into two batches, the first batch was milled raw undicorticated with a hammer mill to produce raw sandbox meal, the second batch was ...

  14. Effects of in ovo supplementation of probiotics on performance and immunocompetence of broiler chicks to an Eimeria challenge.

    Science.gov (United States)

    Pender, C M; Kim, S; Potter, T D; Ritzi, M M; Young, M; Dalloul, R A

    2016-11-30

    Coccidiosis is regarded as the parasitic disease with the greatest economic impact on the poultry industry due to reduced performance and increased mortality. A study was conducted to investigate the effects of in ovo administration of probiotics on hatchability, performance, immune organ weights, and lesion scores in broiler chicks during a mixed Eimeria infection. At embryonic day 18, 210 eggs were injected with either sterile water or 1×10 6 cfu probiotic bacteria. On day 3 post-hatch, half of the chicks from each treatment group were challenged with a mixed inoculum of Eimeria acervulina, Eimeria maxima and Eimeria tenella. Measurements and tissue samples were taken on day of hatch (DOH) and days 3, 9 and 15. On day 9, 24 birds per treatment were scored for intestinal Eimeria lesions. No differences were seen among groups for hatchability as well as for body weight (BW), BW gain (BWG), or immune organ weights prior to the Eimeria challenge. On day 9, the non-challenged birds with probiotic supplementation had higher BW and BWG than the non-supplemented controls while no differences were seen among the challenged groups. On day 15, probiotic supplemented birds had improved BW compared to the non-supplemented birds as well as increased BWG from day 9 to 15. Bursa weight was not affected by treatment at any time point while spleen weight was greater in supplemented birds on day 15. Birds receiving the probiotic had significantly lower mortality than non-treated birds. Additionally, gross lesion severity was reduced due to probiotic supplementation in all intestinal segments evaluated. These results suggest that in ovo supplementation of probiotics may improve early performance and provide protection against a mixed Eimeria infection.

  15. Growth performance, duodenal morphology and the caecal microbial population in female broiler chickens fed glycine-fortified low protein diets under heat stress conditions.

    Science.gov (United States)

    Awad, E A; Idrus, Z; Soleimani Farjam, A; Bello, A U; Jahromi, M F

    2018-03-09

    1. This study was undertaken to examine the effect of feeding glycine (Gly)-fortified low protein (LP) diets on the growth performance, duodenal morphology and caecal microbial populations of broiler chickens raised under unheated, cyclic or constant heat stress environmental conditions. 2. From d 1 to 21 (starter phase), an equivalent number of birds were fed either a normal protein (NP) diet or a LP diet fortified with Gly. From d 22 to 42 (grower phase), an equivalent number of birds from each starter diet were distributed to one of the following dietary groups: (i) an NP diet during the starter and grower phases (NPNP), (ii) an NP diet during the starter phase and a LP diet during the grower phase (NPLP), (iii) an LP diet during the starter phase and an NP diet during the grower phase (LPNP) or (iv) LP diets during both phases (LPLP). 3. Commencing from d 22, an equivalent number of birds from each dietary group were exposed to (i) 23 ± 1°C throughout (unheated), (ii) 34 ± 1°C for 7 h each day from 10:00 to 17:00 (cyclic heat) or (iii) 34 ± 1°C throughout (constant heat). 4. Feeding the LP diet during the starter phase resulted in feed intake (FI), weight gain (WG), feed conversion ratios (FCR) and energy efficiency ratios (EER) similar to those for the NP diet. The birds fed the LP diet had a significantly higher protein efficiency ratio (PER) compared with the birds fed the NP diet. 5. During the grower phase, there were significant diet × temperature interactions for F, WG, FCR, PER, EER, villus height, crypt depth and caecal Clostridia. The birds fed the NPLP and LPLP diets had lower FI, WG and EER, higher FCR, shorter villus height and crypt depth and higher caecal Clostridia compared with the birds fed LPNP and NPNP diets under constant heat stress. However, feeding birds the NPLP and LPLP diets resulted in FI, WG, EER, FCR, morphology parameters and caecal Clostridia equivalent to the birds fed LPNP and NPNP diets, as well as improved PER

  16. Effect of dietary supplementation with Rhizopus oryzae or Chrysonilia crassa on growth performance, blood profile, intestinal microbial population, and carcass traits in broilers exposed to heat stress

    OpenAIRE

    S. Sugiharto; T. Yudiarti; I. Isroli; E. Widiastuti; F. D. Putra

    2017-01-01

    Dietary supplementation of additives has recently been part of strategies to deal with the detrimental effects of heat stress (HS) on the performance and carcass traits in broiler chicks. This study aimed to investigate the effect of dietary supplementation with the fungi Rhizopus oryzae or Chrysonilia crassa on growth, blood profile, intestinal microbial population and carcass traits in broiler chicks subjected to HS. R. oryzae and C. crassa are filamentous fungi isolated from...

  17. Blood chemistry changes in broiler chickens following supplementation with Cinnamomum zeylanicum

    Directory of Open Access Journals (Sweden)

    Faixová Zita

    2008-01-01

    Full Text Available A study was conducted to investigate the effects of different doses of Cinnamomum zeylanicum in diet on blood biochemistry of broiler chickens. Thirty two, 1-d old male broiler chickens of a commercial strain ROSS 308 were distributed into groups of 8 birds in each one. The chicks received the diets from the day of hatching to 38 d of age. The four types of diets included basal diets for chicks (HYD 01, HYD 02 and HYD 03 supplemented by 0%, 0.1%, 0.05 and 0.025% cinnamon (Cinnamomi aetheroleum of Cinnamomum zeylanicum, Calendula a.s., Nová L'ubovña, Slovakia. Continuous lighting and water and feed ad libitum were provided throughout the trial. The addition of cinnamon to the diets caused a significantly lower plasma glucose level and the effects of cinnamon on plasma glucose levels tended to be dose-dependent. Dietary intake of 0.05 and 0.025% cinnamon reduced serum ALT and plasma potassium levels. Ingestion of cinnamon, however, resulted in no significant changes in circulating calcium, albumin, triglycerides, free glycerol and cholesterol levels. It was concluded that cinnamon could be used not only for flavor and taste in food preparation but it had an additional role in glucose metabolism in broiler chickens.

  18. Comparative utilization of shea butter cake and palm kernel cake by ...

    African Journals Online (AJOL)

    A 2 x 3 factorial experiment combining two factors, SBC and PKC at three levels of inclusion (5%, 10% and 15%) was designed. The six dietary treatments were fed to a total of 180 day-old chicks up to 56 days or age. Broilers fed SBC diets consumed more feed (P < 0.05) than those fed PKC diets between 0-28 days or age.

  19. Meat quality characteristics of sexed broiler chickens reared on ...

    African Journals Online (AJOL)

    This study determined meat quality characteristics of 300 sexed Arbor Acre broiler chickens reared on deep-litter and deep-litter with a run housing systems. After brooding for 2 weeks, a total of 75 male and female chicks, respectively were confined on deep litter and on deep litter with a run having three replications of 25 ...

  20. Interrelations between the microbiotas in the litter and in the intestines of commercial broiler chickens.

    Science.gov (United States)

    Cressman, Michael D; Yu, Zhongtang; Nelson, Michael C; Moeller, Steven J; Lilburn, Michael S; Zerby, Henry N

    2010-10-01

    The intestinal microbiota of broiler chickens and the microbiota in the litter have been well studied, but the interactions between these two microbiotas remain to be determined. Therefore, we examined their reciprocal effects by analyzing the intestinal microbiotas of broilers reared on fresh pine shavings versus reused litter, as well as the litter microbiota over a 6-week cycle. Composite ileal mucosal and cecal luminal samples from birds (n = 10) reared with both litter conditions (fresh versus reused) were collected at 7, 14, 21, and 42 days of age. Litter samples were also collected at days 7, 14, 21, and 42. The microbiotas were profiled and compared within sample types based on litter condition using PCR and denaturing gradient gel electrophoresis (PCR-DGGE). The microbiotas were further analyzed using 16S rRNA gene clone libraries constructed from microbiota DNA extracted from both chick intestinal and litter samples collected at day 7. Results showed significant reciprocal effects between the microbiotas present in the litter and those in the intestines of broilers. Fresh litter had more environmental bacteria, while reused litter contained more bacteria of intestinal origin. Lactobacillus spp. dominated the ileal mucosal microbiota of fresh-litter chicks, while a group of bacteria yet to be classified within Clostridiales dominated in the ileal mucosal microbiota in the reused-litter chicks. The Litter condition (fresh versus reused) seemed to have a more profound impact on the ileal microbiota than on the cecal microbiota. The data suggest that the influence of fresh litter on ileal microbiota decreased as broilers grew, compared with temporal changes observed under reused-litter rearing conditions.

  1. EFFECT OF TWO COMMERCIAL ANTI-STRESS DRUGS ON THE GROWTH OF ARTIFICIALLY INDUCED STRESSED BROILERS

    Directory of Open Access Journals (Sweden)

    A. Memon, N. A. Qureshi, Mol. Rind, A.A. Solangi and G. Memono1

    2001-01-01

    Full Text Available The Study was carried out to evaluate the efficacy of anti-stress commercial drugs (Vitasol Super and Vitamionic-33 on growth of stressed broilers, at the Poultry Experimental Station, Sindh Agriculture University Tandojam during August-September, 1998. A-day old 150 chicks were equally housed in three groups that were A, Band C. In group “A” five grams Vitasol Super was added in 40 litres of drinking water, while in group “B” one gram of Vitaminic-33 was added in three litres of drinking water. Group “C” was kept as control, where no anti-stress drug was supplemented in water. Results revealed highly significant difference among weight gain of broilers fed on ration supplemented with different anti-stress drugs. Average weight gain of all groups A, Band C were 1796.50, 1899.80 and 1760.52 gms, respectively. Average feed consumption of different groups were 3830, 3859 and 3818 gms, respectively. Average feed conversion ratio of different groups A, Band C was 2.14, 2.03 and 2.17, respectively. The average dressing percentage of difference groups were 62.10, 64.52 and 61.60. Highly significant difference was observed in weight of internal organs of different groups. The average per kilogram of broilers profit of different groups were Rs. 10.49, 13.81 and 10.95, respectively. The birds of group B, which was, earned maximum profit given Vitaminic-33 (anti-stress drug. It was concluded that anti-stress vitamin (Vitaminic-33 at the rate of 5grams/40 litres of water ad libitum can be successfully used for better growth of broilers

  2. Phytase supplementation improved growth performance and bone characteristics in broilers fed varying levels of dietary calcium.

    Science.gov (United States)

    Powell, S; Bidner, T D; Southern, L L

    2011-03-01

    An experiment was conducted to investigate the effect of dietary Ca level on the efficacy of phytase. A total of 288 male Ross × Ross 708 broilers with initial and final BW of 37 and 705 g, respectively, were used in brooder batteries from 0 to 21 d posthatch. Each treatment had 8 replications with 6 broilers/replicate pen. All diets were corn-soybean meal based and formulated to contain 1.26% total Lys. The treatments were positive control with 0.45% nonphytate P and 1% Ca and a negative control with 0.20% nonphytate P with 0.67, 1.00, or 1.33% Ca fed with or without 500 phytase units of Optiphos (Escherichia coli-derived phytase; JBS United Inc., Sheridan, IN). Increasing Ca from 0.67 to 1.33% linearly decreased (P ≤ 0.003) ADG, ADFI, bone breaking strength, bone weight, tibia ash weight, and percentage tibia ash; however, quadratic effects were found for ADFI, G:F, percentage tibia ash, and mortality (P ≤ 0.09). Phytase supplementation increased (P ash weight, and percentage tibia ash and decreased (P = 0.054) mortality. The increase in ADG, ADFI, bone weight, ash weight, and percentage tibia ash (P ≤ 0.026) and decrease in mortality (phytase × Ca linear; P = 0.058) from phytase supplementation was greater in broilers fed the higher levels of Ca. Calcium utilization was linearly decreased (P < 0.002) with increasing Ca. Phosphorus digestibility and utilization were increased with increasing levels of Ca (P ≤ 0.002); however, P utilization decreased at 1% Ca and increased at 1.33% (quadratic; P < 0.070). Phytase supplementation increased Ca utilization (P < 0.024), P digestibility (P < 0.001), and P utilization (P < 0.029). However, the increase in P digestibility (phytase × Ca; P < 0.021) was greater at the lower levels of Ca whereas P utilization (phytase × Ca; P < 0.001) was greater at 1.33% Ca with phytase supplementation. The results of this research indicate that dietary Ca level, within the ranges used in this experiment, does not negatively

  3. Effect of enterogermina (Bacillus clausii spores) on the productive performance of broilers

    OpenAIRE

    Núñez Torres, Oscar; Arévalo Castro, Renato P.; Kelly, Gerardo; Guerrero, Jorge R.

    2017-01-01

    The effect of a commercial probiotic, Enterogermina (Bacillus clausii spores) in the drinking water on the productive performance of broilers was evaluated. A total of 280 male broilers, Cobb line, of one day of age were used for 49 days. The chicks were distributed in four treatments with seven replicates per treatment: T0 = balanced feed (control), T1, T2 and T3 = balanced feed + 0.25, 0.50 and 0.75 ml of enterogermina per liter of water, respectively, in a randomized complete block design....

  4. Some microbiological, histopathological and biochemical changes in the broiler chicken fed carrot leaves

    International Nuclear Information System (INIS)

    El-Faramawy, A.A.; Soliman, S.M.; Fahmy, M.O.

    2005-01-01

    Ninety 21 day old Arbor Acres chicks arranged into 6 equal groups were used in the present study to evaluate the effect of substitution of 10% yellow corn (YC) with berseem, green carrot leaves without and with digestive enzymatic mixture, untreated dry carrot leaves and treated ones with Aspergillus niger on the intestinal microflora, histopathology of liver and intestine in addition to some biochemical parameters. Replacement of (YC) with green carrot leaves, untreated and treated carrot leaves caused significant (P<0.05) increase in total bacterial count and significant (P<0.05) decrease in Lactobacilli, while the addition of enzyme to the green carrot leaves led to decrease in total bacterial count and increase in Lactobacillus count. The histopathological changes of both intestine and liver were restricted only to both groups of dried carrot leaves. Similarly untreated and treated dry carrot leaves groups were also associated with significant increase in triglyceride, ALT and significant (P<0.05) decrease in cholesterol in serum. From the above mentioned results, it could be concluded that dried carrot leaves, untreated or treated with Aspergillus niger, were unsuitable for broiler chicken feeding since they directly affected bird's gut health causing change in nutrient absorption and considerable decrease in the beneficial bacterial count

  5. Download this PDF file

    African Journals Online (AJOL)

    Michael

    2014-03-01

    Mar 1, 2014 ... 2 Nutritional Biochemistry, Department of Animal Production and Health, The ... with maize and has equivalent mineral and vitamin density. .... 23.73 g/d on broiler-chicks fed processed pigeon pea meal [16], but surpassed the.

  6. Effects of Moringa oleifera seed oil supplemented diet on ...

    African Journals Online (AJOL)

    One hundred and twenty (120) day old chicks comprising of two broiler genotypes (Arbor Acre and Ross 308) were fed to graded levels (0%, 4.5%, 5% and 5.5%) of Moringa oleifera Seed Oil (MOSO) to investigate its effect on their performance. The experiment was conducted for 35 days and the chickens were fed ad ...

  7. Effect of l-glutamic acid supplementation on performance and nitrogen balance of broilers fed low protein diets.

    Science.gov (United States)

    Bezerra, R M; Costa, F G P; Givisiez, P E N; Freitas, E R; Goulart, C C; Santos, R A; Souza, J G; Brandão, P A; Lima, M R; Melo, M L; Rodrigues, V P; Nogueira, E T; Vieira, D V G

    2016-06-01

    The aim of this study was to evaluate the effect of protein reduction and supplementation of l-glutamic acid in male broiler diets. A total of 648 chicks of the Cobb 500 strain were distributed in a completely randomized design with six treatments and six replications with eighteen birds per experimental unit. The study comprised pre-starter (1-7 days), starter (8-21 days), growth (22-35 days) and final (36-45 days) phases. The first treatment consisted of a control diet formulated according to the requirements of essential amino acids for each rearing phase. The second and third treatments had crude protein (CP) reduced by 1.8 and 3.6 percentage points (pp) in relation to the control diet respectively. In the fourth treatment, l-glutamic acid was added to provide the same glutamate level as the control diet, and in the last two treatments, the broilers were supplemented with 1 and 2 pp of glutamate above that of the control diet respectively. The reduction in CP decreased the performance of broilers and the supplementation of l-glutamic acid did not influence performance when supplied in the diets with excess of glutamate. The lowest excreted nitrogen values were observed in the control diet, and treatments 2 and 3, respectively, in comparison with treatments with the use of l-glutamic acid (5 and 6). Retention efficiency of nitrogen was better in the control diet and in the treatment with a reduction of 1.8 pp of CP. It was verified that the serum uric acid level decreased with the CP reduction. A reduction in CP levels of up to 21.3%, 18.8%, 18.32% and 17.57% is recommended in phases from 1 to 7, 8 to 21, 22 to 35 and at 36 to 42 days, respectively, with a level of glutamate at 5.32%, 4.73%, 4.57%, 4.38%, also in these phases. Journal of Animal Physiology and Animal Nutrition © 2015 Blackwell Verlag GmbH.

  8. Evaluation of Dietary Glycerin Inclusion During Different Broiler Rearing Phases

    Directory of Open Access Journals (Sweden)

    LW Freitas

    Full Text Available ABSTRACT The objective of this study was to evaluate the effect of the dietary addition of different levels of glycerin on the performance, litter moisture, pododermatitis incidence, and carcass and parts yield of broilers. In total, 1,610 broilers were reared in 35 pens with 46 birds each. A completely randomized experimental design, with five treatments with seven replicates was applied. The experimental treatments were: T1: control diet; T2: dietary inclusion of 5% glycerin from 1-42 days of age; T3: dietary inclusion of 10% glycerin from 1-42 days of age; T4: dietary inclusion of 5% glycerin from 7-42 days of age; T5: dietary inclusion of 10% glycerin from 7-42 days of age. The diets containing glycerin fed since the pre-starter period improved broiler weight gain and feed conversion ratio, but did not influence feed intake or livability. At the end of the experiment, the production efficiency index of the broilers fed 10% glycerin during the entire rearing period was significantly reduced compared with the other treatments. Litter moisture in the pens of broilers fed 10% glycerin during the entire rearing period was higher compared to the other treatments since day 21.Diets containing 10% glycerin, both for the entire rearing period (1-42 days or only after the pre-starter phase (7-42 days, influenced broiler performance and incidence of severe pododermatitis, reducing the production efficiency indexes at 42 days. Glycerin may be added up to 5% in broiler´s diets with no effect on performance, litter moisture and carcass yield, indicating that this co-product of the biodiesel industry can be used as an alternative feedstuff for broilers.

  9. Effects of xylanase and citric acid on the performance, nutrient retention, and characteristics of gastrointestinal tract of broilers fed low-phosphorus wheat-based diets

    NARCIS (Netherlands)

    Esmaeilipour, O.; Shivazad, M.; Moravej, H.; Aminzadeh, S.; Rezaian, M.; Krimpen, van M.M.

    2011-01-01

    An experiment was conducted to study the effects of xylanase and citric acid on the performance, nutrient retention, jejunal viscosity, and size and pH of the gastrointestinal tract of broilers fed a low-P wheat-based diet. The experiment was conducted as a 2 × 3 factorial arrangement with 2 levels

  10. Biochemical and Histopathological Effects of Dietary Supplementation of Nigella sativa and Mentha piperita Oils to Broilers

    Directory of Open Access Journals (Sweden)

    Marian H. Ghaly

    2017-01-01

    Full Text Available This study was carried out to evaluate the biochemical and histopathological effects of dietary supplementation of Nigella sativa and Mentha piperita oils to broilers for 6 weeks. A total 80 unsexed one day old Cobb broiler chicks was obtained from Cairo Company with average body weight 55g were used .The chicks were taken and randomly allocated into 4 equal groups (20 each named control, Nigella sativa oil treated group, Mentha piperita oil treated group and Nigella sativa + Mentha piperita oils treated group. Serum samples and tissue samples (liver and kidney were obtained at 21 and 42 days old for some serum biochemical analysis (ALT, AST and ALP activities; creatinine, Urea, Total protein and Albumin levels and histopathological examination (Liver and Kidney processed slide sections. The obtained results indicated that dietary supplementation of broilers diet with Nigella sativa, Mentha piperita and their combination for long period (6 weeks couldn’t alter liver and kidney functions as cleared from biochemical findings but could caused slight hepatic and renal histopathological changes as showed from histopathological examination.

  11. Occurrence of white striping and wooden breast in broilers fed grower and finisher diets with increasing lysine levels.

    Science.gov (United States)

    Cruz, R F A; Vieira, S L; Kindlein, L; Kipper, M; Cemin, H S; Rauber, S M

    2017-02-01

    Two experiments were conducted to evaluate the prevalence and severity of white striping (WS) and wooden breast (WB) in breast fillets from broilers fed diets with increasing digestible Lysine (dLys) from 12 to 28 d (Exp. 1) and from 28 to 42 d (Exp. 2). Trials were sequentially conducted using one-d-old male, slow-feathering Cobb 500 × Cobb broilers, both with 6 treatments and 8 replicates. Increasing dLys levels were equally spaced from 0.77 to 1.17% in Exp. 1 and from 0.68 to 1.07% in Exp. 2. The lowest dLys diet was not supplemented with L-Lysine (L-Lys) in either one of the studies and all other essential amino acid (AA) met or exceeded current commercial recommendations such that their dietary concentrations did not limit broiler growth. Four birds per pen were randomly selected from each replication and processed at 35 and 42 d in Exp. 1 and Exp. 2, respectively. Deboned breast fillets (Pectoralis major) were submitted to a 3 subject panel evaluation to detect the presence of WS and WB, as well as to provide scores of WS (0-normal, 1-moderate, 2-severe) and WB (0-normal, 1-moderate light, 2-moderate, 3-severe). Increasing the level of dLys had a positive effect on BW, carcass, and breast weight, as well as breast yield. White striping and WB prevalences were 32.3 and 85.9% in Exp. 1 and 87.1 and 89.2% in Exp. 2. Birds fed diets not supplemented with L-Lys had the lowest average WS and WB scores (0.22 and 0.78 in Exp. 1 and 0.61 and 0.68 in Exp. 2). White striping and WB presented linear responses to performance variables in Exp. 1, whereas quadratic responses were observed for all variables in Exp. 2. In conclusion, increasing the level of dLys improved growth performance and carcass traits as well as induced the occurrence and severity of WS and WB lesions. © 2016 Poultry Science Association Inc.

  12. The effects of Saccharomyces cerevisiae on the morphological and biomechanical characteristics of the tibiotarsus in broiler chickens

    Directory of Open Access Journals (Sweden)

    B. Suzer

    2017-12-01

    Full Text Available The aim of this study is to examine the effects of different levels of the feed supplement Saccharomyces cerevisiae, a yeast metabolite, on broiler tibiotarsus traits and to reduce leg problems by identifying the pathological changes in leg skeletal system. Thus, reducing leg disorders due to the skeletal system, the cause of significant economic losses in our country (Turkey, was investigated by the supplementation of Saccharomyces cerevisiae in broiler feed. In the study, 300 male day-old, Ross 308 broiler chicks were used. Experiment groups were designed as follows: control; 0.1 % Saccharomyces cerevisiae; 0.2 % Saccharomyces cerevisiae; 0.4 % Saccharomyces cerevisiae. The experimental diets were chemically analyzed according to the methods of the Association of Official Analytical Chemists. Twelve groups were obtained, including three replicates for each experimental group. Each replicated group was comprised of 25 chicks, and thus 75 chicks were placed in each experimental group. After 42 days, broiler chickens were slaughtered. Tibiotarsi were weighed with a digital scale, and the lengths were measured with a digital caliper after the drying process. Cortical areas were measured with the ImageJ Image Processing and Analysis Program. A UTEST Model-7014 tension and compression machine and a Maxtest software were used to determine the bone strength of the tibiotarsus. The severity of the tibial dyschondroplasia lesion was evaluated as 0, +1, +2 and +3. Crude ash, calcium and phosphorus analyses were performed to determine the inorganic matter of tibiotarsi. For radiographic evaluations of epiphyseal growth plates, tibiotarsi from the right legs were photographed in lateral and craniocaudal positions and examined. Statistical analyses were performed with the SPSS statistics program. It was observed that the use of Saccharomyces cerevisiae as a feed supplement led to an increase in the bone traits of broiler chickens. Optimum

  13. The Effect of Alone or combined Dietary Supplementation of ...

    African Journals Online (AJOL)

    kurt

    A study was conducted to investigate the effect of dietary supplementation of an organic acid, a probiotic or a ... performance and slaughter characteristics of broiler chickens fed a maize-soya based diet. The six .... Bird density was. 12 chicks ...

  14. Leucine and valine requirements of the growing germfree chicks

    International Nuclear Information System (INIS)

    Ishibashi, Teru; Kametaka, Masao; Ozaki, Akira; Yamamoto, Tetsuzo; Mitsuoka, Tomotari.

    1977-01-01

    To compare the requirements of leucine and valine of the growing germfree and conventional chicks, 7-day-old chicks were fed a diet with graded levels of leucine or valine for 7 days. Daily gains of body weight of the germfree chicks were greater than those of the conventional chicks when the dietary leucine or valine level was higher than the requirement level. Adversely, daily gains of body weight of the germfree chicks were less than those of the conventional chicks when the dietary leucine or valine level was very low. Leucine and valine requirements for maximum growth, however, was the same and estimated to be 0.95 and 0.78%, repectively, of the diet for both groups. After the feeding test, the chicks were injected with L-leucine- and L-valine-U- 14 C and expired carbon dioxide was collected for 2 and 3 hours, respectively. From the percentage of recovery of 14 C in the expired carbon dioxide, the leucine and valine requirements were found to be 0.80 and 0.53% of the diet for both groups, respectively. Three hours after feeding test, the plasma free valine concentration was estimated in the chicks which was not injected the isotope. The requirement of valine was estimated to be 0.59% for the germfree chicks and 0.54% for the conventional chicks. From the above data, it may be concluded that no difference, or very small if any, is found in the requirements of leucine and valine between the germfree chicks and those of the conventional chicks. (auth.)

  15. The effect of partial substitution of roasted soybean seed with graded levels of sweet potato (Ipomoea batatas) leaf meal on growth performances and carcass characteristics of broiler chickens.

    Science.gov (United States)

    Melesse, Aberra; Alemu, Temesgen; Banerjee, Sandip; Berihun, Kefyalew

    2016-06-30

    This study was designed to assess the effects of partial substitution of roasted soybean seed with sweet potato (Ipomoea batatas) leaf meal (SPLM) on growth performances and carcass components of broiler chickens. The experiment was a completely randomized design consisting of five dietary treatments replicated four times with ten chicks each. The control diet (treatment 1, T1) contained roasted soybean seed as the major protein source without SPLM and treatment diets containing SPLM at the levels of 30 g/kg (treatment 2, T2), 60 g/kg (treatment 3, T3), 90 g/kg (treatment 4, T4) and 120 g/kg (treatment 5, T5) by partially substituting the roasted soybean seed in the control diet. The results indicated that the body weight gain in chickens reared in T1 was (pchickens reared in T1 was (pchickens fed on T1 was (pChickens fed with T2 diet had numerically higher values of slaughter, dressed carcass and breast than those reared in T4 and T5 diets. It can be concluded that the partial substitution of roasted soybean seed with sweet potato leaf might be economically feasible feeding strategy of broiler chickens in smallholder poultry farm settings.

  16. Use of Sunflower Meal with Enzyme Mixture Substituted for Soybean Meal in Broiler Diets

    OpenAIRE

    KIRKPINAR, Figen; BASMACIOĞLU, Hatice

    2001-01-01

    The study was carried out to investigate the effects of sunflower meal with an enzyme mixture containing a-amylase, ß-glucanase, cellulase, lipase and protease substituted for soybean meal in broiler diets on performance, intestinal weight, viscosity and pH, as well as the frequency of sticky droppings and abdominal fat. In the trial, a total of 960 one-day-old Avian broiler chicks were used. The trial lasted 6 weeks. A total of 12 experimental diets were supplied ad libitum. Dietary treatme...

  17. Nigella seed oil as alternative to avilamycin antibiotic in broiler ...

    African Journals Online (AJOL)

    This study was conducted to study the effect of nigella seed oil as an alternative to the antibiotic, avilamycin, on growth, digestibility, muscle fatty acid profile and some physiological parameters in broiler chickens. Fifty four chicks at 15 d of age (average weight, 320 ± 3 g) were divided into a control group and two treatment ...

  18. Physico-chemical shelf-life indicators of meat from broilers given ...

    African Journals Online (AJOL)

    The objective of the study was to determine the effect of using Moringa oleifera leaf meal (MOLM) as an additive on physico-chemical shelf life indicators of meat from broilers. A total of 432 1-day-old chicks were randomly allocated to four treatments (TRT's). Water and feed was provided ad libitum. The feeding phases were ...

  19. Effect of Feeding Low-Oil Ddgs to Laying Hens and Broiler Chickens on Performance and Egg Yolk and Skin Pigmentation

    Directory of Open Access Journals (Sweden)

    A Cortes-Cuevas

    2015-06-01

    Full Text Available Two experiments were conducted to evaluate the nutritional quality of two sources of low-oil distiller's dried grains with solubles (DDGS and their pigmenting ability for broiler chicken skin and egg yolks. In Experiment 1, 360 Bovan-White hens between 69 and 77 weeks of age were randomly assigned to five dietary treatments with 6 replicates of 12 hens each. In Experiment 2, 375 Ross 308 broiler chickens were randomly assigned to five treatments with three replicates of 25 birds each. The chickens were fed the experimental diets from one to 42 d of age. In both experiments, treatments consisted of a basal diet with no DDGS, and diets with 6% or 12% inclusion of DDGS from two sources. In Experiment 1, no significant differences in performance were detected among treatments (p> 0.05. Egg yolk pigmentation, according to CR-400 Minolta Colorimeter redness (a and yellowness (b, linearly increased (p0.05 were detected among treatments in growth performance, carcass yield, or abdominal fat at 42 d of age. Yellowness linearly increased (p<0.05 in the skin and abdominal fat of the birds that consumed diets with DDGS. The results of the current study indicate that feeding two sources of low-oil DDGS to broiler chicks or laying hens does not negatively affect egg production or growth performance while improves egg yolk and skin yellowness.

  20. Effect of Calcium and Phosphorus Ratios in Feed on the Specific Radioactivity of 32P Recovered in Excreta and Blood Plasma by Growing Broiler Chicks

    International Nuclear Information System (INIS)

    Al-Masri, M.R.

    1998-01-01

    32 P was injected into male broiler chicks aged 14 days. The experiment lasted until day 29. During the experiment specific radioactivity of 32 P recovered in excreta and in plasma were quantitatively evaluated at different Ca:P ratios (I=1:1; II=1.5:1; III=2:1; IV=2.5:1) in four groups given experimental diets. The phosphorus content was the same in all groups. Specific radioactivity of 32 P in excreta and in plasma amounted to (Bq/mg P):614, 588, 193 and 180; and 928, 964, 1103 and 1136 in groups(I,II,III and IV ) respectively. Widening of the Ca:P ratio in the feed decreased the radio of 32 P specific radioactivity in excreta to 32 P specific radioactivity in plasma

  1. Effect of Phytase Superdoses and Citric Acid on Growth Performance, Plasma Phosphorus and Tibia Ash in Broilers Fed Canola Meal-Based Diets Severely Limited in Available Phosphorus

    Directory of Open Access Journals (Sweden)

    Taheri HR

    2015-06-01

    Full Text Available This experiment was conducted to investigate the effect of phytase superdoses alone or in combination with citric acid (CA in canola meal-based diets severely limited in available phosphorus (Pa on growth performance, plasma phosphorus (P, and tibia ash (TA in broilers from 22 to 42 d of age. Two hundreds and eighty 21-d-old male broilers were used in 28 pens of 10 birds per each. The experimental diets consisted of a positive control (PC diet and six negative control (NC diets which consisted of two levels of CA (0 and 20 g/Kg and three levels of phytase (0, 1000 and 4000 U/Kg in a 2 × 3 factorial arrangement. The PC diet contained 4.3 g/Kg Pa, but all NC diets contained 1.5 g/Kg Pa. Results indicated that the birds fed the PC diet had a significantly higher average daily gain (ADG, plasma P and TA, but a lower feed conversion ratio (FCR than those fed the NC diet. The ADG, FCR and plasma P values in birds fed NC diets supplemented with 4000 U/Kg phytase enzyme (with or without CA significantly reached those of birds fed the PC diet. But, addition of phytase enzyme at 1000 U/Kg only plus CA to the NC diet could significantly improve FCR and plasma P. A significant interaction was observed between phytase and CA for FCR and plasma P. Although TA values in NC + 1000 U/Kg phytase treatments (with or without CA were similar to the PC treatment, TA values of NC + 4000 U/Kg phytase treatments (with or without CA was greater than that of the PC treatment. Results of this study showed that, in severely limited Pa corn-canola meal-based diets, supplementing 4000 U/Kg phytase or also 1000 U/Kg phytase plus CA will be sufficient to obtain the comparable feed efficiency in broilers to those fed the adequate Pa diet.

  2. Nonshivering thermogenesis and adaptation to fasting in king penguin chicks.

    Science.gov (United States)

    Duchamp, C; Barre, H; Delage, D; Rouanet, J L; Cohen-Adad, F; Minaire, Y

    1989-10-01

    The ability to develop nonshivering thermogenesis (NST) and the effect of fasting on thermogenic response to cold were studied in winter-acclimatized king penguin chicks. Metabolic rate (MR) and integrated electrical muscle activity were measured at different ambient temperatures. In cold-acclimatized (5 degrees C) fed chicks, shivering threshold temperature (STT) was 9.4 degrees C lower than lower critical temperature (LCT), indicating that NST (0.7 W/kg) occurs at moderate cold, whereas in control chicks fed and reared at 25 degrees C for 3 wk, LCT and STT were similar. Chicks reared in the cold and fasting for 3 wk or 4-5 mo (natural winter fast) developed an NST of 0.8 and 2.4 W/kg, respectively, despite the fast. In fasting chicks, the intercept of the metabolic curve with the abscissa at zero MR was far below body temperature, contrasting with the classic model for heat loss. Their low LCT indicates the capacity of a large reduction in convective conductance characteristic of diving animals and allows energy sparing in moderate cold. Below LCT, conductance reincreases progressively, leading to a steeper than expected slope of the metabolic curve and allowing preservation of a threshold temperature in the shell. These results show for the first time in a wild young bird the development of NST after cold acclimatization. Further, at the temperature of cold acclimatization, an energy-sparing mechanism is shown in response to long-term fast adaptation.

  3. Effect of feeding cottonseed meal on some hematological and serum biochemical parameters in broiler birds

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    G. Thirumalaisamy

    2016-07-01

    Full Text Available Aim: The study was undertaken to find out the effect of feeding cottonseed meal (CSM on performance of hematological and serum biochemical parameters in broiler birds. Materials and Methods: A 6-week biological trial was carried out with 432-day-old Cobb 400 broiler chicks distributed to nine experimental diets with six replicates, each containing eight chicks. The experimental diets were formulated based on total amino acids (BTAA or based on digestible amino acids (BDAA with or without iron supplementation with two levels of CSM (2% and 4% and control diet based on maize – soybean. The whole blood was subjected to hematological studies. The serum samples were analyzed for protein fractions and lipid profiles. Results: The packed cell volume (PCV value, red blood cell (RBC numbers, and hemoglobin (Hb were lower in iron unsupplemented CSM BTAA or BDAA diets than the control (33.86-35.54 vs. 36.41%, 2.78-2.87 vs. 2.98 × 106/μl, and 10.30-10.70 vs. 10.88%. Supplementation of iron in CSM diets improved the PCV, RBC numbers, and Hb, and the values were comparable to the control. White blood cell numbers, mean corpuscular volume, mean corpuscular Hb (MCH, and MCH concentration values were comparable to the control. The erythrocyte osmotic fragility (EOF was poor in birds fed diets containing up to 4% CSM BTAA or CSM BDAA without iron supplementation (32.02-32.57 vs. 28.77%. Supplementation of iron improved the EOF. The serum cholesterol level did not change with or without iron supplementation. Conclusion: This study suggested that feeding of CSM BTAA or BDAA up to 4% level voiding iron supplementation lowers the hematological parameters, whereas supplementation of iron did not alter serum protein fractions and cholesterol profile; however, it had lowered some hematological parameters, which was rectified by iron supplementation.

  4. Effect of using the Matrix Values for NSP-degrading enzymes on performance, water intake, litter moisture and jejunal digesta viscosity of broilers fed barley-based diet

    Directory of Open Access Journals (Sweden)

    Seyed Adel Moftakharzadeh

    2017-02-01

    Full Text Available In this study, we have evaluated the effect of three multi-enzymes nutrient matrix values and compared the results with that fed barley and the corn diets without enzyme. In entire period, addition of all enzymes to the barley-based diet significantly (p 0.05. Litter moisture and water to feed ratio at 15, 25, and 33 days of age significantly decreased by addition of all enzymes (p < 0.05. In conclusion, considering nutrient matrix values for all used enzymes improved performance of broilers and can be used in formulating diets commercial broiler diets based on barley.

  5. The influence of irradiation treatment on gossypol and protein digestibility of decorticated cottonseed meal for Broiler chicks

    International Nuclear Information System (INIS)

    Farag, M.D.H.

    2000-01-01

    The Effect of gamma irradiation (5-20 kGy) on the chemical composition, soluble protein, available lysine, gossypol, in-vitro and in vivo digestibility of decorticated cottonseed meal (CSM) and the performance of broilers fed processed CSM were investigated. Radiation treatment of CSM at dose levels of 5, 10, 15 and 20 kGy did not affect moisture, crude protein, ether extract, crude fiber, ash and NFW content. However, the treatment reduced soluble protein by 4.18, 7.82, 10.91, 13.46%, respectively. Fluorodinitrobenzene-available lysine concentration reduced by 4.48, 8.12, 17.65 and 21.29% respectively. The concentrations of total gossypol decreased by 7.97, 15.78, 19.92 and 25.39% and free gossypol by 3.74, 7.85, 18.43 and 28.01%, respectively. The digestibility of CSM protein was increased by 5.3, 9.0, 16.6 and 17.2%, for in-vitro digestibility and by 4.8, 7.3, 9.4 and 12.9% for in-vivo due to treatment with 5,10, 15 and 20 kGy, respectively. From 7 to 42 days, arbor acres broilers were freely given iso energetic and iso nitrogenous : corn-soy control diet or diets containing 300 g kg -1 raw or irradiated CSM at 4, 15 or 20 kGy. Intake of raw CSM affected growth, feed intake and feed conversion as compared with birds receiving the control diet

  6. Effects of dietary protein levels during rearing and dietary energy levels during lay on body composition and reproduction in broiler breeder females.

    Science.gov (United States)

    van Emous, R A; Kwakkel, R P; van Krimpen, M M; Hendriks, W H

    2015-05-01

    A study with a 2 × 3 × 2 factorial arrangement was conducted to determine the effects of 2 dietary protein levels (high = CPh and low = CPl) during rearing, 3 dietary energy levels (3,000, MEh1; 2,800, MEs1; and 2,600, MEl1, kcal/kg AMEn, respectively) during the first phase of lay, and 2 dietary energy levels (2,800, MEs2; and 3,000, MEh2, kcal/kg AMEn, respectively) during the second phase of lay on body composition and reproduction in broiler breeders. No meaningful interactions for energy and protein treatments within the different phases of the study were found and, therefore, this paper focusses on the main effects. Pullets fed the CPl diet had a 12.8% higher feed intake, 14% lower breast muscle, and 97% higher abdominal fat pad portion at 22 wk age. The increased abdominal fat pad and decreased breast muscle of the CPl compared to the CPh birds increased hatchability during the first phase of lay, due to a decreased embryonic mortality between d 10 to 21 of incubation, and increased egg production during the second phase of lay. Feeding birds the MEh1 and MEl1 diets slightly decreased egg production compared to the MEs1 birds. Birds fed the MEh1 diet showed a higher mortality compared to the birds fed the MEs1 and MEl1 diets. Feeding birds the MEh2 diet did not affect egg production, increased hatchability of fertile eggs, decreased embryonic mortality between d 3 to 21 of incubation, and increased the number of first-grade chicks. It was concluded that a low-protein diet during rearing changed body composition with positive effects on incubation traits during the first phase of lay and improved egg production during the second phase of lay in broiler breeders. A high-energy or low-energy diet compared to a standard diet during the first phase of lay slightly decreased total and settable egg numbers while a high-energy diet during the second phase of lay increased hatchability and number of saleable chicks. © 2015 Poultry Science Association Inc.

  7. Feed contamination with Fusarium mycotoxins induces a corticosterone stress response in broiler chickens.

    Science.gov (United States)

    Antonissen, G; De Baere, S; Devreese, M; Van Immerseel, F; Martel, A; Croubels, S

    2017-01-01

    The aim of the present study was to evaluate the effect of the Fusarium mycotoxins deoxynivalenol (DON) and fumonisins (FBs) on the stress response in broiler chickens, using corticosterone (CORT) in plasma as a biomarker. Chickens were fed either a control diet, a DON contaminated diet, a FBs contaminated diet, or a DON and FBs contaminated diet for 15 d at concentrations close to the European Union maximum guidance levels for DON and FBs in poultry. Mean plasma CORT levels were significantly higher in broiler chickens fed a DON contaminated and a DON and FBs contaminated diet compared to birds fed a control diet. A similar trend was observed for animals fed a FBs contaminated diet. Consequently, feeding broilers a diet contaminated with DON and/or FBs induced a CORT stress response, which may indicate a negative effect on animal welfare. © 2016 Poultry Science Association Inc.

  8. Effects of Protease Addition and Replacement of Soybean Meal by Corn Gluten Meal on the Growth of Broilers and on the Environmental Performances of a Broiler Production System in Greece

    Science.gov (United States)

    Bonos, Eleftherios; Anestis, Vasileios; Filioussis, Georgios; Papanastasiou, Dimitrios K.; Bartzanas, Thomas; Papaioannou, Nikolaos; Tzora, Athina; Skoufos, Ioannis

    2017-01-01

    An experimental study was conducted to examine the combined effects of adding a dietary protease, reducing the levels of soybean meal (SBM) and introducing corn gluten meal (CGM) in the ration of a group of broilers reared on a commercial Greek farm. Five hundred forty chicks were divided into three dietary treatments with six replicates of thirty birds each. The first group (Control) was fed a conventional diet based on corn and soybean meal, containing 21% w/w crude protein (CP). The second group (Soy-Prot) was supplied a corn and SBM-based diet containing a lower level of CP (20% w/w) and 200 mg of the protease RONOZYME® Proact per kg of feed. The third group (Gluten-Prot) was fed a diet without soybean-related constituents which was based on corn and CGM and with CP and protease contents identical to those of the diet of the Soy-Prot group. Body weight, feed intake, feed conversion ratio (FCR), intestinal microbiota populations and morphology, meat quality and cost were evaluated. Furthermore, a partial life cycle assessment (LCA) was performed in order to assess the potential environmental performance of the systems defined by these three dietary treatments and identify their environmental hot-spots. The growth performance of the broilers supplied the Soy-Prot diet was similar to the broilers supplied the Control diet. However, the broilers which were fed the Gluten-Prot diet at the end of the trial showed a tendency (P≤0.010) for lower weight gain and feed intake compared to those of the Control diet. When compared to the Control group, lower counts of C. perfringens (P≤0.05) were detected in the ileum and cecum parts, and lower counts of F. necrophorum (P≤0.001) were detected in the cecum part of the birds from the Gluten-Prot group. The evaluation of intestinal morphometry showed that the villus height and crypt depth values were not significantly different (P>0.05) among the experimental groups for the duodenum, jejunum and ileum parts. No

  9. Effects of Protease Addition and Replacement of Soybean Meal by Corn Gluten Meal on the Growth of Broilers and on the Environmental Performances of a Broiler Production System in Greece.

    Directory of Open Access Journals (Sweden)

    Ilias Giannenas

    Full Text Available An experimental study was conducted to examine the combined effects of adding a dietary protease, reducing the levels of soybean meal (SBM and introducing corn gluten meal (CGM in the ration of a group of broilers reared on a commercial Greek farm. Five hundred forty chicks were divided into three dietary treatments with six replicates of thirty birds each. The first group (Control was fed a conventional diet based on corn and soybean meal, containing 21% w/w crude protein (CP. The second group (Soy-Prot was supplied a corn and SBM-based diet containing a lower level of CP (20% w/w and 200 mg of the protease RONOZYME® Proact per kg of feed. The third group (Gluten-Prot was fed a diet without soybean-related constituents which was based on corn and CGM and with CP and protease contents identical to those of the diet of the Soy-Prot group. Body weight, feed intake, feed conversion ratio (FCR, intestinal microbiota populations and morphology, meat quality and cost were evaluated. Furthermore, a partial life cycle assessment (LCA was performed in order to assess the potential environmental performance of the systems defined by these three dietary treatments and identify their environmental hot-spots. The growth performance of the broilers supplied the Soy-Prot diet was similar to the broilers supplied the Control diet. However, the broilers which were fed the Gluten-Prot diet at the end of the trial showed a tendency (P≤0.010 for lower weight gain and feed intake compared to those of the Control diet. When compared to the Control group, lower counts of C. perfringens (P≤0.05 were detected in the ileum and cecum parts, and lower counts of F. necrophorum (P≤0.001 were detected in the cecum part of the birds from the Gluten-Prot group. The evaluation of intestinal morphometry showed that the villus height and crypt depth values were not significantly different (P>0.05 among the experimental groups for the duodenum, jejunum and ileum parts. No

  10. Effects of Protease Addition and Replacement of Soybean Meal by Corn Gluten Meal on the Growth of Broilers and on the Environmental Performances of a Broiler Production System in Greece.

    Science.gov (United States)

    Giannenas, Ilias; Bonos, Eleftherios; Anestis, Vasileios; Filioussis, Georgios; Papanastasiou, Dimitrios K; Bartzanas, Thomas; Papaioannou, Nikolaos; Tzora, Athina; Skoufos, Ioannis

    2017-01-01

    An experimental study was conducted to examine the combined effects of adding a dietary protease, reducing the levels of soybean meal (SBM) and introducing corn gluten meal (CGM) in the ration of a group of broilers reared on a commercial Greek farm. Five hundred forty chicks were divided into three dietary treatments with six replicates of thirty birds each. The first group (Control) was fed a conventional diet based on corn and soybean meal, containing 21% w/w crude protein (CP). The second group (Soy-Prot) was supplied a corn and SBM-based diet containing a lower level of CP (20% w/w) and 200 mg of the protease RONOZYME® Proact per kg of feed. The third group (Gluten-Prot) was fed a diet without soybean-related constituents which was based on corn and CGM and with CP and protease contents identical to those of the diet of the Soy-Prot group. Body weight, feed intake, feed conversion ratio (FCR), intestinal microbiota populations and morphology, meat quality and cost were evaluated. Furthermore, a partial life cycle assessment (LCA) was performed in order to assess the potential environmental performance of the systems defined by these three dietary treatments and identify their environmental hot-spots. The growth performance of the broilers supplied the Soy-Prot diet was similar to the broilers supplied the Control diet. However, the broilers which were fed the Gluten-Prot diet at the end of the trial showed a tendency (P≤0.010) for lower weight gain and feed intake compared to those of the Control diet. When compared to the Control group, lower counts of C. perfringens (P≤0.05) were detected in the ileum and cecum parts, and lower counts of F. necrophorum (P≤0.001) were detected in the cecum part of the birds from the Gluten-Prot group. The evaluation of intestinal morphometry showed that the villus height and crypt depth values were not significantly different (P>0.05) among the experimental groups for the duodenum, jejunum and ileum parts. No

  11. Prevalence of Coccidiosis in Broiler Chicken Farms in Western Iran

    Directory of Open Access Journals (Sweden)

    Jamal Gharekhani

    2014-01-01

    Full Text Available The main goal of current study was to investigate the prevalence of coccidiosis in broiler farms in Hamedan province, western Iran. Chicks and fecal samples were collected in all of the 220 broiler farms in this region. All viscera were examined for gross pathological changes. The mucosa of small intestine and the caeca were examined for the presence and identification of parasitic forms using parasitology methods. The overall rate of coccidiosis was 31.8%; E. acervulina (75.7%, E. tenella (54.3%, E. necatrix (28.6%, and E. maxima (20% were determined. Mixed infections were observed in all of the positive farms. There was a statistical significant difference (P0.05. This is the first report of coccidiosis rate in broiler farms in this region. Further additional researches and design control strategies for improving management in farms are necessary.

  12. Effect of essential oil compound on shedding and colonization of Salmonella enterica serovar Heidelberg in broilers.

    Science.gov (United States)

    Alali, W Q; Hofacre, C L; Mathis, G F; Faltys, G

    2013-03-01

    The objectives of this study were to determine the effect of an essential oil blend (EO; carvacrol, thymol, eucalyptol, lemon) administered in drinking water on the performance, mortality, water consumption, pH of crop and ceca, and Salmonella enterica serovar Heidelberg fecal shedding and colonization in broiler birds following Salmonella Heidelberg challenge and feed withdrawal. Chicks were randomly assigned to water treatments containing 0.05, 0.025, or 0.0125% EO or untreated controls. Treatments were administered in drinking water on 0 to 7 and 35 to 42 d. One-half of the chicks were challenged with Salmonella Heidelberg and placed in pens with unchallenged chicks on d 1. Performance, mortality, water consumption, and pH were determined during the 42-d study. Prevalence of Salmonella Heidelberg was determined on drag swabs (0, 14, and 42 d) and in the ceca and crops (42 d). The 0.05% EO administered in drinking water significantly (P water significantly lowered the feed conversion ratio and increased weight gain compared with controls, but did not significantly reduce Salmonella Heidelberg colonization in the crops. The EO in drinking water did not significantly reduce Salmonella Heidelberg colonization in ceca or fecal shedding in broilers. The EO used in the study may control Salmonella Heidelberg contamination in crops of broilers when administered in drinking water and therefore may reduce the potential for cross-contamination of the carcass when the birds are processed.

  13. Utilization of supplemental methionine sources by primary cultures of chick hepatocytes

    International Nuclear Information System (INIS)

    Dibner, J.J.

    1983-01-01

    Utilization of 2-hydroxy-4-(methylthio) butanoic acid (HMB) as a substrate for protein synthesis was studied by using primary cultures of chick liver cells. Cultures were prepared by enzymatic dissociation of livers from week old Hubbard broiler chicks and were maintained for 4 days under nonproliferative conditions. Hepatocyte differentiation was verified by using dexamethasone induction of tyrosine aminotransferase activity. Conversion of [14C]HMB to L-methionine was shown by chromatographic analysis of hepatocyte protein hydrolysate and incorporation into protein was proven by cycloheximide inhibition of synthesis. When incorporation of HMB was compared to that of DL-methionine (DLM) equimolar quantities of the two sources were found in liver cell protein. These results support, at a cellular level, the conclusion that HMB and DLM are biochemically equivalent sources of methionine for protein synthesis

  14. Simultaneous oral administration of Salmonella Infantis and S. Typhimurium in chicks.

    Science.gov (United States)

    Murakami, Koichi; Maeda-Mitani, Eriko; Onozuka, Daisuke; Noda, Tamie; Sera, Nobuyuki; Kimura, Hirokazu; Fujimoto, Shuji; Murakami, Satoshi

    2017-01-01

    To confirm the hypothesis that Salmonella enterica subspecies enterica serovar ( S. ) Infantis has higher basic reproductive rates in chicks compared with other Salmonella serovars, 1-day-old specific-pathogen-free chicks ( n  = 8) were challenged simultaneously with S. Infantis and S. Typhimurium per os . Challenged chicks (Group A) were then housed with non-infected chicks (Group B, n  = 4) for 6 days (from 2 to 8 days of age). Group B birds were then housed with other non-infected birds (Group C, n  = 4), which were then transferred to cages containing a further group of untreated chicks (Group D, n  = 2). A control group consisting of four non-infected chicks was used for comparison. All chickens were humanely sacrificed at 18 days of age, and Salmonella from bowel and liver samples were enumerated. Both serovars were isolated from all groups except the control group. S. Typhimurium was isolated at a greater frequency than S. Infantis from the bowel samples of chicks from Groups B, C and D, while no differences in colonisation rates were observed between the two serovars in liver samples from Groups B, C and D. S. Typhimurium, but not S. Infantis, was immunohistochemically detected in the lamina propria of the cecum and rectum in five birds of Group A. Despite the competitive administration, neither of the two serovars completely excluded the other, and no differences were observed in basic reproductive rates between the two serovars. These findings, together with data from previous studies, suggest that the initial quantitative domination of S. Infantis in chicken flocks may explain why this serovar is predominant in broiler chickens.

  15. Heterophil Phagocytic Activity Stimulated by L61 and L55 Supplementation in Broilers with Infection

    Directory of Open Access Journals (Sweden)

    Pairat Sornplang

    2015-11-01

    Full Text Available Newborn chicks are susceptible to Salmonella enterica serovar Enteritidis (SE. The objective of this study was to evaluate the effect of Lactobacillus probiotic isolated from chicken feces on heterophil phagocytosis in broiler chicks. A total of 150 newborn broiler chicks were divided into 5 groups (30 chicks per group as follows: group 1 (normal control, given feed and water only, group 2 (positive control given feed, water and SE infection, group 3 (L61 treated given feed, water, SE infection followed by Lactobacillus salivarius L61 treatment, group 4 (L55 treated given feed, water, SE infection followed by L. salivarius L55 treatment, and group 5 given feed, water, SE infection followed by L. salivarius L61 + L55 combination treatment. After SE infection, L. salivarius treatment lasted for 7 days. The results showed that L. salivarius L61 and L. salivarius L55 treatment, either alone or combination of both, increased the survival rate after SE infection, and upregulated heterophil phagocytosis and phagocytic index (PI. Conversely, chick groups treated with Lactobacillus showed lower SE recovery rate from cecal tonsils than that of the positive control group. The PI values of the chicken group with SE infection, followed by the combination of L. salivarius L61 and L. salivarius L55 were the highest as compared to either positive control or normal control group. Two Lactobacillus strains supplementation group showed significantly (p<0.05 higher PI value at 48 h than 24 h after treatment.

  16. The effect of dietary phosphorus level and phytase supplementation on growth performance, bone-breaking strength, and litter phosphorus concentration in broilers.

    Science.gov (United States)

    Powell, S; Johnston, S; Gaston, L; Southern, L L

    2008-05-01

    This research was conducted to determine the effects of feeding different P levels with and without phytase supplementation on broiler growth performance, bone-breaking strength (BBS), and litter P concentration. An experiment with 4 trials was conducted with 7,840 Ross x Ross straight-run broilers. For each trial, 1,960 broilers were allotted on d 0 to treatments, with 7 replications each and with 70 broilers per replication. The broilers were fed a 4-period feeding program consisting of starter (0 to 14 d), grower (14 to 32 d), finisher (32 to 41 d), and withdrawal (41 to 50 d) periods. For each trial, the same pen was used continuously for each treatment-replication combination, and the litter was not removed between trials. Broilers were fed a control diet [0.43, 0.40, 0.36, or 0.32% nonphytate P (nPP)] in the starter, grower, finisher, and withdrawal periods, respectively, a low Ca and P (LCaP) diet with a 0.05% reduction in nPP in each period, and these 2 diets supplemented with phytase at 600 phytase units/kg (nPP and Ca were reduced by 0.094% in diets with phytase). Diet did not affect (P > 0.10) broiler performance in the starter or withdrawal periods. Generally, both phytase addition and the LCaP diet decreased some aspects of growth performance during the grower and finisher periods. There was no main effect of phytase on BBS, but BBS was decreased in the broilers fed the LCaP diet with phytase addition (nPP x phytase, P broilers fed the LCaP diet. Total P, soluble P (SP), and reactive soluble P (RSP) were decreased (P litter of broilers fed the LCaP diets. Total P, SP, and RSP were decreased (P litter of broilers fed phytase. These data indicate that phytase supplementation at 600 phytase units/kg reduces growth in some periods, has no effect on BBS, and reduces total P, SP, and RSP in the litter.

  17. The physicochemical properties and antioxidative potential of raw thigh meat from broilers fed a dietary medicinal herb extract mixture

    Directory of Open Access Journals (Sweden)

    K. Shirzadegan

    2014-07-01

    Full Text Available A 6-wk feeding study was conducted to evaluate the antioxidative potential, indices such as quality of the thigh meat and liver of broiler chickens fed with a dietary medicinal herb extract mixture (HEM, consisting: Iranian green tea, cinnamon, garlic and chicory at a ratio of 25:15:45:15. A total of 320, one-d-old Ross (male broiler chickens were used to investigate the effects of 0.0, 2.5, 5.0 and 7.5 g/kg HEM in the diet, on aforementioned factors. The HEM supplementation did not influence the composition of raw thigh meat except for the total phenols and crude ash (P<0.05. Furthermore, pH, water-holding capacity (WHC and acceptability of thigh meat were affecting by administration of HEM in diets (P<0.05. Meat flavor increased in the supplemented groups (P<0.05. According to our data, HEM supplementation decreased the amount of thiobarbituric acid reactive substance (TBARS in various times of storage and improved the liver lipid peroxides and superoxide dismutase (SOD activities at week 6 (P<0.05, but did not influence the catalase activity. Our results reveal that the addition of 7.5 g/kg or higher HEM in diet could be sufficient to increase the antioxidative activity and 2.5 g/kg for meat taste of broilers in maximum levels.

  18. Specialized protein products in broiler chicken nutrition: A review

    Directory of Open Access Journals (Sweden)

    Sleman S.M. Beski

    2015-06-01

    Full Text Available In poultry nutrition, most attention is given to protein products, due to the importance of protein as a major constituent of the biologically active compounds in the body. It also assists in the synthesis of body tissue, for that renovation and growth of the body. Furthermore, protein exists in form of enzymes and hormones which play important roles in the physiology of any living organism. Broilers have high dietary protein requirements, so identification of the optimum protein concentration in broiler diets, for either maximizing broiler performance or profit, requires more knowledge about birds' requirements for protein and amino acids and their effects on the birds' growth performance and development. It also requires knowledge about the protein sources available that can be used in poultry diets. The broad aim of this review is to highlight the importance of some of the available high-quality specialized protein products of both animal and plant origins which can be explored for feeding broiler chickens. Minimization of the concentration of anti-nutritional factors (ANFs and supplementation with immunologically active compounds are the main focus of gut health-promoting broiler diets. These diet characteristics are influenced by feed ingredient composition and feed processing. The general hypothesis is that these protein products are highly digestible and devoid of or contain less ANFs. Feeding these products to broiler chicks, especially at an earlier age, can assist early gut development and digestive physiology, and improve broiler growth performance and immunity.

  19. The nutritive value of raw Mucuna pruriens (var. utilis ) for broiler ...

    African Journals Online (AJOL)

    The nutritive value of raw Mucuna pruriens (var. utilis) was studied over a 4-week period using 168 broiler chicks at 28 days of age. They were individually weighed and then randomly allocated to four dietary treatments incorporating, respectively, 0, 50, 100, and 150 g M. pruriens per kg of diet. Feed and water were ...

  20. Effects of Dietary Supplemental Vitamins and Periods of Administration on Growth Performance and Antibody Titre of Broiler Chickens Vaccinated against Newcastle Disease

    OpenAIRE

    Odutayo, O. J.; Sogunle, O. M.; Adeyemi, O.A.; Sonibare, A.O.; Oluwayinka, E.B.; Ekunseitan, D.A.; Safiyu1, K. K.

    2016-01-01

    This study investigated the effects of supplemental vitamins and varying administration periods on growth performance and antibody titre of broiler chickens vaccinated against Newcastle Disease (ND). A total of 300 unvaccinated against ND Arbor Acre day-old chicks were used for the study for 8 wk. Birds were brooded together on day 1 of age, and 30 chicks were selected randomly for evaluating the maternally derived antibody titre against ND. At 2 days of age, the remaining 270 chicks were div...

  1. Biochemical and Heamatological Indices of Broiler Chickens fed ...

    African Journals Online (AJOL)

    SH

    investigate the implications of feeding broiler chickens with mucuna bean processed by simple domestic methods on performance, haematological and biochemical parameters. Materials and Methods. Sample preparation: The raw Mucuna pruriens beans used in this study were purchased from International Institute of.

  2. Effect of an Enzyme Blend on the Performance, Diet Metabolizability, Phosphorous Retention, and Bone Mineralization of Broilers Fed Diets Containing Defatted Rice Bran

    Directory of Open Access Journals (Sweden)

    ML Moraes

    2015-06-01

    Full Text Available An experiment was conducted to evaluate the effect of an enzyme blend (EB on the performance, diet metabolizability, phosphorus (P retention, and bone mineralization of broilers fed diets containing 10% defatted rice bran (DRB. In total, 432 one- to 38-d-old male Cobb broilers were evaluated according to a completely randomized experimental design in 3 x 2 factorial arrangement. Three diets were tested with two nutrient reductions (NR in the matrix (standard diet; NR I of 75 kcal/kg ME, 0.1% Ca and 0.1% available P; and NR II of 100 kcal/kg ME, 0.1% Ca and 0.1% available P with or without the addition of an EB (200 g/t. The coefficients of total tract apparent retention (CTTAR of the diets and P retention were determined by collecting excreta during two periods (14 to 17 and 28 to 31 d. As expected, birds fed the standard diet had higher BW, BW gain, and G:F compared to birds on the NR diets. The EB did not show any positive effects on CTTAR or on performance; however, birds fed the EB retained 6.58% more P from d 14 to 17 (p ≤ 0.07 and 8.55% from d 28 to 31 (p < 0.05. Tibiotarsus ash percentage also increased by 2.45% (p ≤ 0.06 on d 38. In diets containing 10% DRB, the enzyme blend showed biological activity improving P retention and tibiotarsus mineralization.

  3. Molecular diversity of lactic acid bacteria on ileum broiler chicken fed by bran and bran fermentation

    Science.gov (United States)

    Baniyah, Laelatul; Nur Jannah, Siti; Rukmi, Isworo; Sugiharto

    2018-05-01

    Lactic Acid Bacteria (LAB) is a digestive tract microflora that have a positive role in poultry health. The number and diversity of LAB in the digestive tract affected by several factors, among them was the kind of feed. The purpose of this research was to know diversity of Lactic Acid Bacteria (LAB) ileum broiler’s after feeding with prebiotic bran and Rhizopus oryzae fermented bran which was added to commercial feed. As much as 15 broilers were used to determine the diversity of LAB. All broilers were fed using commercial feed. The control used commercial feed no addition of bran or fermented bran, and commercial feed with fermented bran and nonfermented bran were as a treatment. To determine the diversity of LAB, T-RFLP method was applied. The Hae III and Msp I were used as restriction enzymes. The number of phylotype, relative abundance, Shannon diversity index (H '), evenness (E), and Dominance (D) were examined. The results indicated that the addition of prebiotic bran on commercial feed showed a higher diversity of lactic acid bacteria on broiler’s ileum, compared with control and addition of Rhizopus oryzae fermented bran. LAB group that dominates in the ileum is Lactobacillus sp. and L. delbruecii subs bulgaricus.

  4. Performance and some carcass measurements in starter broiler ...

    African Journals Online (AJOL)

    A 28 day feeding trial was conducted to investigate the performance and some carcass measurements in starter broiler birds fed sweet potato meal. Three hundred and five (305) Anak broilers of one week old were randomly assigned to five treatment diets. Diets 1, 2, 3, 4, and 5 contain 0, 2.5, 5.0, 7.5 and 10.0% levels of ...

  5. Growth curves and their implications in hand-fed Monk parrots (Myiopsitta monachus

    Directory of Open Access Journals (Sweden)

    Petzinger C

    2015-10-01

    Full Text Available Christina Petzinger,1,2 J Jill Heatley,3 John E Bauer1,2 1Comparative Animal Nutrition Research Laboratory, Department of Small Animal Clinical Sciences, College of Veterinary Medicine and Biomedical Sciences, 2Intercollegiate Faculty of Nutrition, Texas A&M University, 3Zoological Medicine Service, Department of Small Animal Medicine and Surgery, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, College Station, TX, USA Abstract: Monk parrots (Myiopsitta monachus were hand-fed over two chick seasons spanning of 2010 to 2011. Information from the growth curve of chicks hand-fed in 2010 was used to develop a feeding protocol for the 2011 season (Protocol-2011. This protocol addressed the problems of delayed followed by excessive growth experienced by parrots hand fed in 2010. Monk parrots that were hand-fed in 2011 following the new protocol experienced delayed growth after 20 days of age. However, some Monk parrots were fed in excess of Protocol-2011 and did not experience a major delay in growth. The energy requirement equations used to construct Protocol-2011 were low when compared to adult Monk parrot maintenance energy requirements. The data suggest that growing birds do not require approximately twice their adult maintenance energy requirements, as is the case for growing dogs. Additionally, there appear to be fluctuations in energy needs as Monk parrots grow. A major increase in energy needs occurred between days 18 and 23 posthatching, which corresponds to feather development and growth in Monk parrot chicks. Thus, multiple equations estimating energy requirements, rather than just one equation, are likely needed from hatching to fledging in order to ensure adequate energy is provided to chicks. More research on the energy requirements of growing Monk parrots, especially around the time of fledging and weaning, is needed to improve hand-fed methods and potentially the adult health of hand-fed birds. Keywords

  6. Dietary sodium bicarbonate, cool temperatures, and feed withdrawal: impact on arterial and venous blood-gas values in broilers.

    Science.gov (United States)

    Wideman, R F; Hooge, D M; Cummings, K R

    2003-04-01

    Sodium bicarbonate (NaHCO3) has been used successfully in mammals and birds to alleviate pulmonary hypertension. Experiment 1 was designed to provide measurements of arterial and venous blood-gas values from unanesthetized male broilers subjected to a cool temperature (16 degrees C) challenge and fed either a control diet or the same diet alkalinized by dilution with 1% NaHCO3. The incidences of pulmonary hypertension syndrome (PHS, ascites) for broilers fed the control or bicarbonate diets were 15.5 and 10.5%, respectively (P = 0.36, NS). Non-ascitic broilers fed the control diet were heavier than those fed the bicarbonate diet on d 49 (2,671 vs. 2,484 g, respectively); however, other comparisons failed to reveal diet-related differences in heart weight, pulse oximetry values, electrocardiogram amplitudes, or blood-gas values (P > 0.05). When the data were resorted into categories based on right:total ventricular weight ratios (RV:TV) indicative of normal (RV:TV or = 0.28) pulmonary arterial pressures, broilers with elevated RV:TV ratios had poorly oxygenated arterial blood that was more acidic, had high partial pressure of CO2 (PCO2), and had higher HCO3 concentrations when compared with broilers with normal RV:TV ratios. Experiment 2 was conducted to determine if metabolic variations associated with differences in feed intake or environmental temperature potentially could mask an impact of diet composition on blood-gas values. Male broilers maintained at thermoneutral temperature (24 degrees C) either received feed ad libitum or had the feed withdrawn > or = 12 h prior to blood sampling. Broilers fed ad libitum had lower venous saturation of hemoglobin with O2, higher venous PCO2, and higher arterial HCO3 concentrations than broilers subjected to feed withdrawal. Broilers in experiment 2 fed ad libitum and exposed to cool temperatures (16 degrees C) had lower arterial partial pressure of O2 and higher venous PCO2 than broilers fed ad libitum and maintained at 24

  7. Effect of high-dose phytase and citric acid, alone or in combination, on growth performance of broilers given diets severely limited in available phosphorus.

    Science.gov (United States)

    Taheri, H R; Jabbari, Z; Adibnia, S; Shahir, M H; Hosseini, S A

    2015-01-01

    1. Two trials were conducted to evaluate the effect of high-dose phytase alone or in combination with citric acid (CA) in the diet severely limited in available phosphorus (P) on performance, plasma P and plasma Ca of broilers from 22 to 42 d of age. 2. In Trial 1, 297 21-d-old female chicks were placed into 27 pens and allocated to 9 maize-soybean meal-based dietary treatments, which were a positive control [PC, 4.23 g/kg non-phytate P (NPP)] and 8 negative control (NC, 1.35 g/kg NPP) groups consisting of two concentrations of CA (0 and 20 g/kg) and 4 concentrations of phytase (0, 1000, 2000 and 4000 U/kg) in a 2 × 4 factorial arrangement. In Trial 2, 192 21-d-old male chicks were placed into 24 pens and allocated to 6 wheat-canola meal-based dietary treatments, which were a PC (4.2 g/kg NPP), a NC (1.68 g/kg NPP) and 4 NC groups consisting of two concentrations of CA (0 and 20 g/kg) and two concentrations of phytase (2000 and 4000 U/kg) in a 2 × 2 factorial arrangement. 3. In both trials, birds fed on the PC had significantly higher average daily gain (ADG), average daily feed intake (ADFI), plasma P and lower feed conversion ratio (FCR) and plasma Ca than those of birds fed on the NC. CA supplementation significantly increased ADG and ADFI. There was a significant interaction between CA and phytase on plasma P where CA improved the effect of phytase on plasma P. In Trial 1, phytase addition improved ADG, ADFI, FCR and plasma Ca linearly. 4. Briefly, this research showed the interaction effect between CA and phytase on plasma P when broilers were fed on diets based on maize-soybean meal or wheat-canola meal. The results showed that CA supplementation lowered the concentration of phytase that is needed in low NPP diets to increase plasma P.

  8. Effects of the tricothecene mycotoxin diacetoxyscirpenol on egg production of broiler breeders.

    Science.gov (United States)

    Brake, J; Hamilton, P B; Kittrell, R S

    2002-12-01

    Three experiments were conducted to determine the effects of 4,15-diacetoxyscirpenol (DAS) on egg quality and egg production of broiler breeders. In Experiment 1, feed containing 0, 1.25, 2.5, or 5.0 mg DAS/ kg was fed from 67 to 69 wk of age followed by a 3-wk recovery period on a slat-litter floor. In Experiment 2, individually caged broiler breeder females were studied from 23 to 31 wk of age. The basal diet containing 0, 5, 10, or 20 mg DAS/kg was fed from 25 to 27 wk of age. In Experiment 3, individually caged broiler breeder hens were studied from 23 to 32 wk of age. DAS was fed at levels of 0 (basal), 5, 10, and 20 mg DAS/kg for 2 wk beginning at Week 24, followed by the basal breeder diet for 7 wk. Egg production was not affected by levels of up to 5 mg DAS/kg in the older hens of Experiment 1. When fed from 25 to 27 wk of age in Experiment 2, DAS decreased egg production at the 20 mg/kg level only. When fed from 24 to 25 wk of age in Experiment 3, DAS had no significant effect on egg production or egg quality. Short-term consumption of DAS at levels that might naturally occur appears to have little effect on broiler breeder egg production.

  9. Effects of Aloe vera on dressing percentage and haemato-biochemidal parameters of broiler chickens

    Directory of Open Access Journals (Sweden)

    Jagmohan Singh

    2013-09-01

    Full Text Available Aim: To evaluate the effects of Aloe vera on dressing percentage and hemato-biochemical parameters of broiler chickens.Materials and Methods: A total of 90 chicks were used in this study. They were randomly allocated into 3 treatment groups.Fresh Aloe vera leaf juice (ALJ was prepared and administered to the test group T3 at the rate of 20 g/Lin drinking water daily.This study was carried out for 42 days. Dressing percentage and hemato-biochemical parameters were recorded at the end ofexperiment.Results: Group that was given Aloe vera (T3 showed numerically higher dressing percentage as compared to control group(T1 and drug control group (T2. It also showed significantly (P0.05 differences were observed in other parameters among all the treatment groups.Conclusion: Aloe vera has potential to be a growth promoter in broiler chicks and its growth promoting effects are comparableto that of antibiotic growth promoter (AGP.

  10. Biogas production: litter from broilers receiving direct-fed microbials and an enzyme blend

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    Maria Fernanda Ferreira Menegucci Praes

    Full Text Available ABSTRACT The effect of additives used in the feed of broilers on anaerobic bio-digestion of poultry litter was evaluated. Four diets were used: NC: negative control; DFM: NC + 500 ppm direct-fed microbials (DFM containing Bacillus subtilis and Bacillus licheniformis; ENZ: diet formulated with an enzyme blend (20 ppm phytase, 200 ppm protease and 200 ppm xylanase; DFM+E: ENZ + DFM. Substrates for the anaerobic bio-digestion were prepared with litter from each treatment, containing 4 % total solids (TS. These were used in 16 continuous bio-digesters with a 2 kg d−1 load, to determine the production and potential biogas production and composition during an 85-day period. Influent and effluent samples were collected for the amounts of TS and volatile solids (VS, fiber fraction (neutral detergent fiber [NDF], acid detergent fiber [ADF] and lignin, nutrients (N, P and K, and total and thermotolerant coliforms to be determined. For all treatments a reduction in the following effluents was observed as follows: TS (49, 48, 48 and 50 % VS (70, 54, 55 and 62 % NDF (91, 90, 95 and 96 % ADF (89, 88, 93 and 94 % and lignin (80, 76, 89 and 88 %. The efficiency of the treatment for coliforms in bio-digesters was higher than 90 % in the 85-day period in all treatment groups. There was a reduction in biogas and methane production when DFM (5500 and 4000 mL and DFM + E (5800 and 4100 mL were used, compared to treatments NC (6300 mL and 4400 and ENZ (6400 and 4500 mL. The potential production of reduced TS and VS was higher in ENZ (1:00 and 1.74 106 mL kg−1 when compared to NC (0.88 and 1:02 106 mL kg−1, DFM (0.80 and 1:40 106 mL kg−1 and DFM + E (0.88 1:25 and 106 mL kg−1. The additives did not affect the percentage of methane production, and all treatments showed values higher than 70 %. Adding enzymes to the diet of broilers influences the litter characteristics and, as a consequence, increases biogas production. The addition of DFM and DFM + E to

  11. Effects of fumonisin B1 and mycotoxin binders on growth performance, tibia characteristics, gut physiology, and stress indicators in broiler chickens raised in different stocking densities.

    Science.gov (United States)

    Lee, S; Kim, D-H; Keum, M-C; Han, E; An, B-K; Chang, H-H; Choi, Y-H; Moon, B-H; Lee, K-W

    2018-03-01

    The present study was carried out to investigate the effects of stocking density, fumonisin B1 (FB), and mycotoxin binder (TB) on growth performance, bone quality, physiological stress indicators, and gut health in broiler chickens. Day-old Ross 308 male broiler chicks (n = 1,440/experiment) were randomly allocated to 72 floor pens in a 3 × 2 × 2 factorial arrangement, using 3 stocking densities (12.5 birds/m2 [HSD], 10 birds/m2 [MSD], or 7.5 birds/m2 [LSD]), 2 levels of purified FB (0 or 10 ppm), and 2 levels of TB (0 or 0.2%). Each treatment had 6 replicates (n = 6/treatment) and experiments lasted 34 days. All data were analyzed using 3-way ANOVA with stocking density level, FB, and TB as main factors. Body weight gain and feed intake were lower (P FB-contaminated diet exhibited a higher feed-to-gain ratio compared with those fed an FB-free diet (P FB-contaminated diet compared with the control diet-fed counterparts. Significant interaction (P FB and TB on serum NO levels was noted. In summary, increasing stocking density lowered growth performance and bone quality, but increased the H/L ratio. Dietary TB did not affect FB-induced increases in the feed-to-gain ratio. No interaction was observed between stocking density and FB for the measured variables.

  12. Effect of dietary nutrients on ileal endogenous losses of threonine, cysteine, methionine, lysine, leucine and protein in broiler chicks.

    Science.gov (United States)

    Cerrate, S; Vignale, S K; Ekmay, R; England, J; Coon, C

    2018-04-01

    An isotope dose technique was utilized (i) to determine endogenous amino acid (AA) and protein losses and (ii) to propose adjusted values for AA requirements. The endogenous flow rate was calculated from the pool of enrichment in plasma AA, assuming similitude to enrichment of endogenous AA. In experiment 1, chicks were orally administered D4-lysine at 2% of estimated lysine intake from 16 to 24 days to find the isotopic steady state of the atom percent excess (APE) of lysine for plasma and jejunal and ileal digesta. The APE of D4-lysine in plasma, jejunal digesta and ileal digesta reached the isotopic steady state at 5.5, 3.4 and 2.0 days, respectively, by using the broken-line model. It was assumed that the isotopic steady state at 5 days identified for D4-lysine is also representative for the 15N-labeled AA. In experiment 2, chicks were fed diets from 1 to 21 days with increasing levels of fat (6%, 8%, 12%, 13% extract ether), protein (26%, 28.5%, 31% CP) or fiber (14%, 16%, 18% NDF) by adding poultry fat, soybean meal, blended animal protein or barley. Chicks were orally administered 15N-threonine, 15N-cysteine, 15N-methionine, 15N-lysine and 15N-leucine at 2% of estimated daily intake for 5 days from 17 to 21 days of age. Dietary nutrients influenced endogenous losses (EL), where dietary fat stimulated EL of lysine (P=0.06), leucine and protein (P=0.07); dietary protein enhanced EL of leucine and protein; and finally the dietary fiber increased EL of leucine. Dietary nutrients also affected apparent ileal digestibility (AID). Dietary fat increased AID of cysteine but decreased AID of lysine. Dietary protein reduced AID of protein, threonine, lysine and leucine, and similarly dietary fiber decreased AID of protein, threonine, methionine, lysine and leucine. In contrast, dietary fat or protein did not affect real ileal digestibility (RID) of protein and AA except threonine and leucine. The dietary fiber reduced the RID of protein, threonine and leucine. This

  13. Biochemical studies in experimentally Escherichia coli infected broiler chicken supplemented with neem (Azadirachta indica leaf extract

    Directory of Open Access Journals (Sweden)

    Vikash Sharma

    2015-11-01

    Full Text Available Aim: An experimental study was conducted on 192-day-old broiler chicks for evaluating the effect of 10% neem leaf extract (NLE supplementationon biochemical parameters in chickens experimentally infected with Escherichia coli O78 at 107 CFU/0.5 ml at 7 days of age. Materials and Methods: The 192-day-old broiler chicks were procured. These chicks were divided into two groups (A and B containing 96 birds each on the 1st day. Diet of all the chicks of Group A was supplemented with 10%NLE in water, whereas chicks of Group B were given feed and water devoid of NLE supplementation throughout the experiment. After rearing for 1 week, chicks of both the groups (A and B were again divided into two subgroups (Group A into A1 and A2 and Group B into B1 and B2 of 54 and 42 birds, respectively. At the age of 7 days all the chicks of groups A1 and B1 were injected with E. coli O78 at 107 CFU/0.5 ml intraperitoneally. Blood samples were collected from six chicks from each group at day 0, 2, 4, 7, 14, 21, 28 days post-infection and serum was separated for biochemical studies. Results: There was a significant increase in serum alanine transaminase (ALT, aspartate transaminase (AST, lactate dehydrogenase (LDH activities, globulin concentration and a decrease in total protein (TP, albumin concentrations, and alkaline phosphatase (ALP activity in both the infected groups. However, the changes in biochemical values, i.e., ALT, AST, LDH, ALP, TP, albumin, and globulin wereof lower magnitude in NLE supplemented group suggesting hepatoprotective and cardioprotective effect of NLE. Conclusions: Fromthe present study, it is reasonable to conclude that significant increase in the value of ALT, AST, LDH, globulin, and significant decrease in the value of ALP, TP, and albumin was of lower magnitude in supplemented infected group (A1 as compared to non-supplemented infected group (B1 suggesting hepatoprotective and cardioprotective effect of NLE.

  14. Standardized Ileal Amino Acid Digestibility of Commonly Used Feed Ingredients in Growing Broilers

    Science.gov (United States)

    Ullah, Zafar; Ahmed, Gulraiz; Nisa, Mehr un; Sarwar, Muhammad

    2016-01-01

    This experiment was conducted to determine standardized ileal amino acid digestibility (SIAAD) of commonly used feed ingredients in poultry diets in Pakistan. These feed ingredients included corn, rice broken (RB), rice polishings (RP), wheat bran (WB), sunflower meal (SFM), cottonseed meal (CSM), guar meal (GM), soybean meal (SBM) from India and Argentine and fish meal (FM). The SIAAD of each ingredient was determined in triplicate using 21-days-old broilers. Day-old male broiler chicks (Hubbard× Hubbard) were reared on corn-SBM based diet from 1 to 13 days and thereafter birds were fed experimental diets from day 14 to 21. Each diet was fed to 36 birds kept in six replicate cages, each cage had six birds. In cereals, the SIAAD of corn’s amino acid (AA) (90.1%) was similar (p>0.05) to RB (89.0%). Isoleucine (97.8%) and lysine (96.9%) were highly digestible AA in corn and RB, respectively. Among cereal-by products, WB’s SIAAD (76.9%) was same (p>0.05) as RP (71.9%). Arginine from WB (82.5%) and RP (83.2%) was highly digestible. However, threonine in WB (72.7%) and leucine in RP (69.6%) were the lowest digestible AAs. In plant protein meals, AAs from Argentine-SBM (85.1%) and Indian-SBM (83.4%) had higher (pdigestible from indispensable AAs. In SFM, methionine (91.4%) SIAAD was the greatest. The average SIAAD of FM was 77.6%. Alanine from FM had the highest (84.0%) but cysteine (62.8%) had the lowest SIAAD. In conclusion, cereals i.e. corn and RB had higher (p0.05). The SBM from plant protein meals had higher (p<0.05) SIAAD than other studied feed ingredients. However, the GM had the lowest (p<0.05) SIAAD among protein meals. PMID:26954227

  15. Heterophil Phagocytic Activity Stimulated by Lactobacillus salivarius L61 and L55 Supplementation in Broilers with Salmonella Infection.

    Science.gov (United States)

    Sornplang, Pairat; Leelavatcharamas, Vichai; Soikum, Chaiyaporn

    2015-11-01

    Newborn chicks are susceptible to Salmonella enterica serovar Enteritidis (SE). The objective of this study was to evaluate the effect of Lactobacillus probiotic isolated from chicken feces on heterophil phagocytosis in broiler chicks. A total of 150 newborn broiler chicks were divided into 5 groups (30 chicks per group) as follows: group 1 (normal control), given feed and water only, group 2 (positive control) given feed, water and SE infection, group 3 (L61 treated) given feed, water, SE infection followed by Lactobacillus salivarius L61 treatment, group 4 (L55 treated) given feed, water, SE infection followed by L. salivarius L55 treatment, and group 5 given feed, water, SE infection followed by L. salivarius L61 + L55 combination treatment. After SE infection, L. salivarius treatment lasted for 7 days. The results showed that L. salivarius L61 and L. salivarius L55 treatment, either alone or combination of both, increased the survival rate after SE infection, and upregulated heterophil phagocytosis and phagocytic index (PI). Conversely, chick groups treated with Lactobacillus showed lower SE recovery rate from cecal tonsils than that of the positive control group. The PI values of the chicken group with SE infection, followed by the combination of L. salivarius L61 and L. salivarius L55 were the highest as compared to either positive control or normal control group. Two Lactobacillus strains supplementation group showed significantly (p<0.05) higher PI value at 48 h than 24 h after treatment.

  16. Presence of antimicrobial resistance in coliform bacteria from hatching broiler eggs with emphasis on ESBL/AmpC-producing bacteria.

    Science.gov (United States)

    Mezhoud, H; Chantziaras, I; Iguer-Ouada, M; Moula, N; Garmyn, A; Martel, A; Touati, A; Smet, A; Haesebrouck, F; Boyen, F

    2016-08-01

    Antimicrobial resistance is recognized as one of the most important global health challenges. Broilers are an important reservoir of antimicrobial resistant bacteria in general and, more particularly, extended-spectrum β-lactamases (ESBL)/AmpC-producing Enterobacteriaceae. Since contamination of 1-day-old chicks is a potential risk factor for the introduction of antimicrobial resistant Enterobacteriaceae in the broiler production chain, the presence of antimicrobial resistant coliform bacteria in broiler hatching eggs was explored in the present study. Samples from 186 hatching eggs, collected from 11 broiler breeder farms, were inoculated on MacConkey agar with or without ceftiofur and investigated for the presence of antimicrobial resistant lactose-positive Enterobacteriaceae, particularly, ESBL/AmpC-producers. Escherichia coli and Enterobacter cloacae were obtained from the eggshells in 10 out of 11 (10/11) sampled farms. The majority of the isolates were recovered from crushed eggshells after external decontamination suggesting that these bacteria are concealed from the disinfectants in the egg shell pores. Antimicrobial resistance testing revealed that approximately 30% of the isolates showed resistance to ampicillin, tetracycline, trimethoprim and sulphonamides, while the majority of isolates were susceptible to amoxicillin-clavulanic acid, nitrofurantoin, aminoglycosides, florfenicol, neomycin and apramycin. Resistance to extended-spectrum cephalosporins was detected in eight Enterobacteriaceae isolates from five different broiler breeder farms. The ESBL phenotype was confirmed by the double disk synergy test and blaSHV-12, blaTEM-52 and blaACT-39 resistance genes were detected by PCR. This report is the first to present broiler hatching eggs as carriers and a potential source of ESBL/AmpC-producing Enterobacteriaceae for broiler chicks.

  17. The effect of yeast β-glucan on the amount of albumin, globulin, urea and total protein of broiler chickens

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    ali kargarirezapour

    2013-08-01

    Full Text Available Glucans derived from yeast cell wall are promising alternatives to antibiotics, as they have been shown to improve growth performance and stimulate the immune system of immature broilers. In this study we evaluated the effect of different levels of yeast beta-glucan (YBG on some blood parametrs of broiler chickens. In a factorial experiment based on completely randomized design (the first factor: YBG levels: 0, 0.04 and 0.08% of basal diet and sex as a second factor 144 day old chicks (72 male and 72 female were selected and allocated to different treatments (three replicates of each treatment. The overall experimental period was 34 days. At the end of study, two birds from each pen were randomly selected as a sample. The level of albumin, globulin, urea and total protein was measured on blood samples. Statistical analysis of the results showed that the YBG had no significant effect on albumin, globulin, urea and total protein level. But the amount of plasma albumin and total protein in female chicks was significantly higher than male chicks (p

  18. Relationship Between Broiler Body Weights, Eimeria maxima Gross Lesion Scores, and Microscores in Three Anticoccidial Sensitivity Tests.

    Science.gov (United States)

    Barrios, Miguel A; Da Costa, Manuel; Kimminau, Emily; Fuller, Lorraine; Clark, Steven; Pesti, Gene; Beckstead, Robert

    2017-06-01

    Anticoccidial sensitivity tests (ASTs) serve to determine the efficacy of anticoccidial drugs against Eimeria field isolates in a controlled laboratory setting. The most commonly measured parameters are body weight gain, feed conversion ratio, gross intestinal lesion scores, and mortality. Due to the difficulty in reliably scoring gross lesion scores of Eimeria maxima , microscopic analysis of intestinal scrapings (microscores) can be used in the field to indicate the presence of this particular Eimeria. The goal of this study was to determine the relationship between E. maxima microscores and broiler body weights and gross E. maxima lesion scores in three ASTs. Day-old broiler chicks were raised for 12 days on a standard corn-soy diet. On Day 12, chicks were placed in Petersime batteries and treatment diets were provided. There were six birds per pen, four pens per treatment, and 12 treatments, for a total of 288 chicks per AST. The treatments were as follows: 1) nonmedicated, noninfected; 2) nonmedicated, infected; 3) lasalocid, infected; 4) salinomycin, infected; 5) diclazuril, infected; 6) monensin, infected; 7) decoquinate, infected; 8) narasin + nicarbazin, infected; 9) narasin, infected; 10) nicarbazin, infected; 11) robenidine, infected; and 12) zoalene, infected. On Day 14, chicks were challenged with an Eimeria field isolate by oral gavage. On Day 20, broilers were weighed, and gross lesion scores and microscores were classified from 0 to 4 depending on the severity of the gross lesion scores and E. maxima microscores. Data from three trials using different field isolates were statistically analyzed using a logarithmic regression model. There was no relationship (P = 0.1224) between microscores and body weight gain. There was a positive relationship between microscores and gross lesion scores (P = 0.004). However, there was also an interaction between isolate and treatment (P Eimeria or the amount of E. maxima in the inoculum.

  19. The influence of the selection of macronutrients coupled with dietary energy density on the performance of broiler chickens

    Science.gov (United States)

    Chrystal, Peter V.; Cowieson, Aaron J.; Truong, Ha H.; Moss, Amy F.; Selle, Peter H.

    2017-01-01

    A total of 360 male Ross 308 broiler chickens were used in a feeding study to assess the influence of macronutrients and energy density on feed intakes from 10 to 31 days post-hatch. The study comprised ten dietary treatments from five dietary combinations and two feeding approaches: sequential and choice feeding. The study included eight experimental diets and each dietary combination was made from three experimental diets. Choice fed birds selected between three diets in separate feed trays at the same time; whereas the three diets were offered to sequentially fed birds on an alternate basis during the experimental period. There were no differences between starch and protein intakes between choice and sequentially fed birds (P > 0.05) when broiler chickens selected between diets with different starch, protein and lipid concentrations. When broiler chickens selected between diets with different starch and protein but similar lipid concentrations, both sequentially and choice fed birds selected similar ratios of starch and protein intake (P > 0.05). However, when broiler chickens selected from diets with different protein and lipid but similar starch concentrations, choice fed birds had higher lipid intake (129 versus 118 g/bird, P = 0.027) and selected diets with lower protein concentrations (258 versus 281 g/kg, P = 0.042) than birds offered sequential diet options. Choice fed birds had greater intakes of the high energy diet (1471 g/bird, P broiler chickens were offered diets with different energy densities but high crude protein (300 g/kg) or digestible lysine (17.5 g/kg) concentrations. Choice fed birds had lower FCR (1.217 versus 1.327 g/g, P broiler chickens selected equal amounts of the three diets in the combination. Regardless of feeding regimen, the intake paths of starch and protein are very close to the null path; however, lipid and protein intake paths in choice fed birds are father from the null path than sequentially fed birds. PMID:29053729

  20. Experimental infection of one-day-old chicks with Salmonella Serotypes Previously isolated from poultry facilities, wild birds, and swine

    Directory of Open Access Journals (Sweden)

    E de Sousa

    2013-12-01

    Full Text Available In order to maintain the high production and export rates achieved by the Brazilian poultry industry, it is necessary to prevent and control certain disease agents, such as Salmonella spp. Using bacterial cultures, the aim of the present study was to investigate the prevalence of Salmonella spp. in specimens collected from broiler facilities. Local wild birds were also sampled, as well as the feces of swine housed on the poultry farm. After sample collection, the isolated serotypes were subsequently inoculated into broiler chicks to determine their effects. Positive samples were collected from the following locations in the poultry facilities: poultry litter (S. serotype 4,5,12:R:-; S. Heidelberg; S. Infantis, broiler feces (S. Heidelberg; S. serotype 6,7:R:-; S. serotype 4,5,12:R:-; S. Tennessee, water (S. Glostrup; S. serotype 6,8:d:-;, and lesser mealworms (Alphitobius diaperinus found in the litter (S. Tennessee. Among the 36 wild birds captured, S. Heidelberg was isolated from one bird's organs and intestinal contents (Colaptes campestris, and S. Enteritidis was isolated from another bird's intestinal contents (Zenaida auriculata. Salmonella Panama and Salmonella Typhimurium were isolated from swine feces. One-day-old chicks (150 were divided into 10 groups of 15 animals each. Each group was orally inoculated with a previously isolated serotype of Salmonella. Soft stools were observed on the cage floor and around the birds' cloaca between 3 and 12 days post-infection (dpi. The different serotypes of Salmonella used to inoculate the chicks were re-isolated from the spleen, liver, and cecal content samples of the infected birds on 15 and 21 dpi.

  1. Effects of Dietary Zinc Oxide and a Blend of Organic Acids on Broiler Live Performance, Carcass Traits, and Serum Parameters

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    BG Sarvari

    2015-12-01

    Full Text Available ABSTRACT This experiment was carried out to evaluate the effect of different dietary supplementation levels of zinc oxide and of an organic acid blend on broiler performance, carcass traits, and serum parameters. A total of 2400 one-day-old male Ross 308 broiler chicks, with average initial body weight 44.21±0.19g, was distributed according to a completely randomized design in a 2 x 3 factorial arrangement. Six treatments, consisting of diets containing two zinc oxide levels (0 and 0.01% of the diet and three organic acid blend levels (0, 0.15, and 0.30% were applied, with eight replicates of 50 birds each. The experimental diets were supplied ad libitum for 42 days. There were significant performance differences among birds fed the different zinc oxide and organic acid blend levels until 42 d of age (p<0.01. The result of this experiment showed that the organic acid blend did not affect feed intake, but zinc oxide increased feed intake. Carcass traits were not influenced by the experimental supplements. Zinc oxide supplementation increased serum alkaline phosphatase level (p<0.01. The organic acid blend reduced serum cholesterol and triglyceride levels (p<0.05. No interactions were found between zinc oxide and the organic acid blend for none of the evaluated parameters. We concluded that zinc oxide and the evaluated organic acid blend improve broiler performance.

  2. Mitochondrial phenotypic flexibility enhances energy savings during winter fast in king penguin chicks.

    Science.gov (United States)

    Monternier, Pierre-Axel; Marmillot, Vincent; Rouanet, Jean-Louis; Roussel, Damien

    2014-08-01

    Energy conservation is a key priority for organisms that live in environments with seasonal shortages in resource supplies or that spontaneously fast during their annual cycle. The aim of this study was to determine whether the high fasting endurance of winter-acclimatized king penguin chicks (Aptenodytes patagonicus) is associated with an adjustment of mitochondrial bioenergetics in pectoralis muscle, the largest skeletal muscle in penguins. The rates of mitochondrial oxygen consumption, and ATP synthesis and mitochondrial efficiency (ATP/O ratio) were measured in winter-acclimatized chicks. We used pyruvate/malate and palmitoyl-l-carnitine/malate as respiratory substrates and results from naturally fasted chicks were compared to experimentally re-fed chicks. Bioenergetics analysis of pectoralis muscle revealed that mitochondria are on average 15% more energy efficient in naturally fasted than in experimentally fed chicks, indicating that fasted birds consume less nutrients to sustain their energy-demanding processes. We also found that moderate reductions in temperature from 38°C to 30°C further increase by 23% the energy coupling efficiency at the level of mitochondria, suggesting that king penguin chicks realize additional energy savings while becoming hypothermic during winter. It has been calculated that this adjustment of mitochondrial efficiency in skeletal muscle may contribute to nearly 25% of fasting-induced reduction in mass-specific metabolic rate measured in vivo. The present study shows that the regulation of mitochondrial efficiency triggers the development of an economical management of resources, which would maximize the conservation of endogenous fuel stores by decreasing the cost of living in fasted winter-acclimatized king penguin chicks. © 2014. Published by The Company of Biologists Ltd.

  3. Effect Of Palm Oil Supplementation On The Performance Of Broiler ...

    African Journals Online (AJOL)

    A 21-day feeding trial was conducted to evaluate the effect of palm oil supplementation on the performance of broiler starter birds fed palm kernel meal based diets. One hundred and twenty (120) one-week-old Anak broilers were randomly assigned to five (5) treatment diets, each treatment diet was replicated four times at ...

  4. Qualidade da carne de frangos de corte submetidos a dietas suplementadas com crômio, criados em diferentes temperaturas ambientais - DOI: 10.4025/actascianimsci.v29i2.218 Meat quality of broiler chicks submeted the supplemented diets with chromium and reared in differents enviromental temperatures - DOI: 10.4025/actascianimsci.v29i2.218

    Directory of Open Access Journals (Sweden)

    Nívea Maria Brancaci Zeoula

    2007-11-01

    Full Text Available Foram realizados dois experimentos. O experimento um objetivou avaliar a composição da carne do peito de frangos alimentados com dietas suplementadas com crômio (0, 400, 800 e 1.200 μg Cr kg-1 complexado a levedura, criados sob diferentes condições de temperatura (quente, termoneutra e fria. O experimento dois objetivou avaliar a qualidade da carne do peito de frangos alimentados com dietas suplementadas com crômio (0 e 400 μg Cr kg-1, criados nas mesmas condições do experimento um e desossados em diferentes períodos (0, 5 e 24 horas. Os resultados mostram que as aves criadas em ambiente frio apresentaram a carne com menor porcentagem de matéria mineral e crômio e maior intensidade de vermelho, enquanto as aves criadas em temperatura termoneutra apresentaram carne com menor porcentagem de umidade, e as aves criadas em temperatura quente, carne com maior luminosidade. Já a suplementação de crômio não proporcionou qualquer efeito na composição e na qualidade de carne, enquanto que o aumento no período de desossa proporcionou um menor comprimento de sarcômero, força de cisalhamento e intensidade de amarelo, porém uma maior luminosidade.Two experiments were conducted being the objective of the first experiment to evaluate the the composition of breast meat of broiler chicks fed with diets supplemented with chromium yeast (0; 400; 800 and 1,200 μg Cr kg-1 reared in different environmental temperatures (hot, thermoneutral and cold. The second experiment aimed to evaluate the quality of breast meat of broiler chicks fed with diets supplemented with chromium yeast (0 and 400 μg Cr kg-1, reared in different environmental temperatures (hot, thermoneutral and cold and deboned in different periods (0, 5 and 24 hours. The results showed that birds reared in cold environmental temperature showed meat with lower mineral matter percentage and chromium and higher values of redness, whereas the birds reared in thermoneutral environment

  5. Various levels and forms of dietary α-lipoic acid in broiler chickens: Impact on blood biochemistry, stress response, liver enzymes, and antibody titers.

    Science.gov (United States)

    Kim, D W; Mushtaq, M M H; Parvin, R; Kang, H K; Kim, J H; Na, J C; Hwangbo, J; Kim, J D; Yang, C B; Park, B J; Choi, H C

    2015-02-01

    The present experiment was conducted to evaluate the impact of various levels and forms of α-lipoic acid (ALA) on blood biochemistry, immune and stress response, and antibody titers in broiler chickens. The four levels (7.5, 15, 75, and 150 ppm) and 2 sources (powder, P-ALA and encapsulated, E-ALA) of ALA along with negative (C-) and positive control (C+; contains antibiotics) diets consisted of 10 dietary treatments, and these treatments were allocated to 1,200 1-d-old chicks and were replicated 12 times with 10 birds per replicate. Among the blood biochemistry parameters, creatinine levels were almost 3 times lower in E-ALA-supplemented diets compared to the C- diet (0.09 vs. 0.25 mg/dL; PBirds did not respond to infectious bronchitis virus (IBV) vaccination at any observed stage (P>0.05). The concentration of cortisol was reduced in chickens fed ALA-supplemented diets as compared to the C- diet (Pbiochemistry profiles and immune responses and reduced stress in broiler chickens. The encapsulated form of ALA was more effective than the powder form. © 2015 Poultry Science Association Inc.

  6. Desempenho de pintos de corte alimentados com rações contendo milho pré-gelatinizado Performance of broiler chicks fed pre-gelatinized corn in pre-starter and starter feed

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    José Henrique Stringhini

    2009-09-01

    corn for broilers from one to 21 days of age. In the first experiment, the metabolism of pre-gelatinized corn, corn starch and corn germ meal was assessed by the total excreta collection method from 4 to 7 days of age and values of apparent (AME and corrected metabolizable energy (CAME and the results were, respectively: 3370, 3379.7, 3259.1 kcal/kg de AME and 3299.8, 3369.7, 3051.6 kcal/kg for CAME. The inclusion level of pre-gelatinized corn was evaluated in pre-starter (1 to 7 days of age - Experiment 2 and starter (8 to 21 days of age - Experiment 3 broiler rations. Broiler performance from one to seven days of age was not affected by pre-gelatinized corn in the feed. A quadratic effect for the dry matter digestibility coefficient (minimum point of 28.6% inclusion and nitrogen (minimum point of 31.7% inclusion and negative linear negative on the ether extract digestibility coefficient. From 8 to 21 days of age, broiler performance was affected linearly and negatively for final weight, weight gain and feed intake and quadratic for feed-to-gain ratio (maximum point of 34.3% inclusion. For the dry matter, nitrogen and ether extract digestibility coefficients, there was a positive linear effect for pre-gelatinized corn included in substitution of corn. Pre-gelatinized corn was a good alternative for pre-starter ration, but, in spite of the better digestibility coefficient results, performance got worse as pre-gelatinized corn increased in diets from 8 to 21 days of age. Pre-gelatinized corn was a good alternative as energetic ingredient for first week chick feed, but, in the starter phase it did not promote good performance despite the good digestibility indexes obtained.

  7. Histomorphometry and macroscopic intestinal lesions in broilers infected with Eimeria acervulina.

    Science.gov (United States)

    Assis, R C L; Luns, F D; Beletti, M E; Assis, R L; Nasser, N M; Faria, E S M; Cury, M C

    2010-03-25

    This study aimed at measuring intestinal villi and assessing the intestinal absorptive area in broilers infected with Eimeria acervulina under different treatments to control coccidiosis. The experiment was divided into two stages, carried out in successive housings, raised in the same environment (or aviary). In the first stage, on 25 May 2008, fifty 12-day-old birds were orally inoculated with 3 x 10(3) oocysts of E. acervulina. In the second stage, on July 2008, other 50 birds were allocated on litter contaminated by the feces of birds on the first housing (natural infection by oocysts present in the reused litter). The experiment was arranged in a complete randomized design with five treatments and three replicates of 10 chicks per treatment. Broiler chicks were housed at 1 day of age and autopsies were performed at 21 days of age. Three 2-cm-long segments of the duodenum were excised from each bird and fixed in 10% buffered formalin. A total of 30 slides were prepared for each treatment, totaling 150 evaluated histological sections using H&E staining. Villus morphology was carried out by the HL Image 97 software. The intestinal absorptive area was calculated and macroscopic lesions were classified according to standard lesion scores. Results showed that intestinal villus measurements and absorptive area are directly affected by E. acervulina and that there is direct and positive correlation between the macro and microscopic findings observed in intestinal coccidiosis. E. acervulina causes shortening of villi and reduction in the intestinal absorptive area, affecting broiler growth. The prevention method of litter fermentation during the interval between housings and oral administration of Diclazuril can reduce the severity of intestinal lesions by E. acervulina in broilers impairing oocyst virulence or viability.

  8. The effects of adding agrimony and sage extracts to water on blood biochemistry and meat quality of broiler chickens

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    Peter Supuka

    2015-01-01

    Full Text Available The aim of our study was to determine the effects of supplementation of agrimony extract (Agrimonia eupatoria L. and a combination of agrimony with sage extract (Salvia officinalis L. to water during the fattening period of broiler chickens on selected biochemical and antioxidant indicators in blood, and on the nutritional composition and oxidative stability of meat. A total of 117 Cobb 500 chicks were randomly divided on the day of hatching into three groups (n = 39 in each and fattened for 42 days. All groups were fed the same diets. In experimental group A water was supplemented with agrimony extract (0.2%. In experimental group AS water was supplemented with a combination of agrimony (0.1% and sage (0.1% extracts. Group C was control without supplementation. The total cholesterol, low density lipoproteins and malondialdehyde in serum were decreased (P P P < 0.05 in both experimental groups compared to control. Our results indicate that supplementation of agrimony and sage extract to water can beneficially influence the antioxidant status as well as oxidative stability of thigh meat and thus improve meat quality. This is a first similar study comparing addition of plant extracts to water in broiler nutrition.

  9. Carcass and internal organ characteristics of brioler chickens fed ...

    African Journals Online (AJOL)

    One hundred and forty-four (144) broiler chickens were used to evaluate the carcass and internal organ characteristics of broiler chickens fed soybean diet partially replaced with variable levels of raw jackfruit seed meal (RJFSM). The study lasted for 7 weeks. The inclusion levels of RJFSM were 10, 20 and 30% respectively ...

  10. Bio-Chemical Evaluation of Yam Peel Meal for Broiler Chickens ...

    African Journals Online (AJOL)

    The nutritional properties and inclusion levels of yam peel meal (YPM) in broiler diets were investigated. Four diets in which YPM substituted maize at 0, 25, 50 and 75% were fed to 180 Anak broiler-type chickens over a 63-day period. Results indicated that YPM contained 89.74% dry matter (DM), 12.03% crude protein ...

  11. Composto exaurido do cogumelo Agaricus blazei na dieta de frangos de corte Spent mushroom substrate of Agaricus blazei in broiler chicks diet

    Directory of Open Access Journals (Sweden)

    Alexandre Magno Batista Machado

    2007-08-01

    Full Text Available Avaliou-se o efeito da substituição de um antibiótico por diferentes níveis do composto exaurido do cogumelo (CEC Agaricus blazei sobre o desempenho de frangos de corte de 1 a 42 dias de idade. Foram avaliadas sete dietas, seis com adição de CEC (0,0; 0,2; 0,4; 0,6; 0,8 e 1,0% e uma com antibiótico (avilamicina 10 ppm. Foram utilizados 588 pintos de 1 dia de idade, distribuídos em um delineamento inteiramente casualizado, com seis repetições por dieta (três por sexo e 14 aves por unidade experimental. As aves que não receberam qualquer aditivo na dieta apresentaram os piores resultados de desempenho. Níveis de CEC superiores a 0,4% ocasionaram redução gradual no desempenho. O nível de 0,2% de CEC proporcionou o maior ganho de peso e o de 0,21%, a melhor conversão alimentar e o maior consumo de ração. À exceção do rendimento de peito e do rendimento de gordura abdominal, os aditivos não influenciaram os rendimentos de carcaça e dos cortes. O composto exaurido do cogumelo Agaricus blazei pode ser utilizado como aditivo alternativo ao antibiótico no nível de 0,2% da dieta, pois não prejudicou o desempenho produtivo e o rendimento de carcaça das aves.The effect of the substitution of an antibiotic by different levels of spent mushroom substrate (SMS of Agaricus blazei on the performance of broiler chicks from 1 to 42 days old was evaluated. Seven diets were evaluated, six with SMS addiction (0.0, 0.2, 0.4, 0.6, 0.8, and 1.0% and one with antibiotic (avilamycin 10 ppm. A total of 588 one day old chicks was allotted to a complete randomized design, with six replications per diet (three per sex and 14 birds per experimental unit. The birds fed diet with any additive showed the worst performance results. Levels of SMS above 0.4% caused gradual reduction in the performance. The level of 0.2% of SMS provided the highest value of weight gain and of 0.21% promoted the best feed conversion and the highest value of feed intake

  12. Ammonia emissions in tunnel-ventilated broiler houses

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    KAO Lima

    2011-12-01

    Full Text Available Gas production in broiler houses and their emissions are closely related to the microclimate established inside the house according to air temperature, humidity, and velocity. Therefore, the internal house environment is influenced by building typology and ventilation system. The objective of the present study was to evaluate ammonia emission rates in broiler houses equipped with different ventilation systems (negative or positive pressure and litter conditions (new or built-up. The environment of six commercial broiler houses was evaluated internal and external NH3 concentrations. Ventilation rates were recorded to estimate ammonia emission rates. The efficiency of circulation and exhaust fans was assessed, and higher ventilation rates were determined in negative-pressure houses due to the higher flow of the fans. Houses with new litter increased ammonia emission rates along the rearing period, indicating the relationship between gas emissions, bird age and ventilation rates, and presented a typical curve of NH3 emission increase. Negative-pressure houses with built-up litter presented higher emission rates during the first rearing week due to the high NH3 concentration during the brooding period, when the ventilation rates required to maintain chick thermal comfort are low. Although the results of the present study indicate an advantage of the positive-pressure systems as to gas emissions, further research is needed reduce gas emissions in broiler houses with negative-pressure systems.

  13. Impact of Skip-a-Day and Every-Day Feeding Programs for Broiler Breeder Pullets on the Recovery of Salmonella and Campylobacter following challenge.

    Science.gov (United States)

    Wilson, K M; Bourassa, D V; McLendon, B L; Wilson, J L; Buhr, R J

    2018-06-08

    The impact of restrictive feeding programs on Salmonella and Campylobacter colonization and persistence after challenge was investigated for broiler breeder pullets housed in an experimental rearing facility. Pullet-chicks were placed on litter in 3 feeding program rooms and each room contained 2 replicate pens. The feeding programs were: (1) Skip-a-day in trough feeders (SAD); (2) Every-day in trough feeders (EDT); (3) Every-day on the pen litter (EDL). On d 1, an additional group of hatchmate chicks were housed in a separate room and gavaged with Salmonella Typhimurium, to later serve as seeder chicks. After seeders were confirmed Salmonella-positive at wk 4, at wk 5 seeders were placed into each feeding program pen to commingle with 135 penmates. At 7, 9, 11, 17, 18, and 20 wk the litter surface in each pen was sampled using intermittently stepped-on drag-swabs. At 8, 12, 16, and 20 wk of age the ceca were sampled from 10 penmates/pen and 2 pooled spleen samples/pen were collected. SAD litter remained Salmonella-positive through 20 wk of age while EDL and EDT pens had no detectible litter Salmonella recovery by 18 and 20 wk. EDL fed pens had no direct (days had significantly higher Salmonella recovery (40%), compared to EDT and EDL (both at 5% recovery). By 16 and 20 wk, only the SAD pullets on the on-feed day (48 h without feed) had recovery of Salmonella at 20%. Salmonella recovery in pooled spleen samples did not appear associated with feeding treatments (22% positive). The remaining pullets challenged with Campylobacter at 21 wk produced similar trends as was seen for Salmonella. SAD program pullets had significantly higher Campylobacter from ceca (80 to 100%) compared to pullets on EDL (30 to 60%) or EDT (40 to 95%). These results suggest that using a Skip-a-Day feeding program for broiler breeder pullets contributes to persistently higher Salmonella and Campylobacter ceca colonization and litter prevalence.

  14. Concentration of sulfur-containing amino acids at turkey broilers during and after muscle dystrophy, fed with deficient feed supplemented with oxidised fat

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    K. Stoyanchev

    2017-06-01

    Full Text Available Abstract. The aim of the present study was to reproduce experimentally muscular dystrophy in 50 broiler turkeys, through early nutrition with a diet deficient in vitamin E, selenium and sulfur-containing amino acids, supplemented with oxidised fat and to study blood plasma sulfur containing amino acids (methionine rd and cysteine. The experiments were conducted with 1 day-old broiler turkeys. By the 3 day of life, they were divided into 40 experimental (II group and 10 control birds (I group; the latter were fed a standard compound feed, whereas the former group received a diet deficient in sulfur-containing amino acids methionine and cysteine (reduced up to 50%, vitamin E, and Se (from 0.2 mg/kg in standard feed to 0.01 mg/kg, further supplemented with oxidized fat containing peroxides and aldehydes with peroxide number of the food 8.0 meq O /kg. The clinical signs of experimental muscle dystrophy in broiler turkeys 2 th appeared first by the 25 day of feeding, when the mild clinical form (II A group and the severe clinical form (II B group werewas established. The results indicated clearly that in turkey broilers suffering from muscle dystrophy, the concentrations of sulfur-containing amino acids cysteine and methionine decreased. After the treatment of turkey broilers withmuscle dystrophy, and supplementation with non-deficient forage with Se, vitamin E, but also with sulfurcontaining amino acids cysteine ormethionine with Seled at a dosage 0.06mg/kg, the plasma levels of sulfur-containing amino acids cysteine andmethionine ® was normalized in the mild clinical form (II A group. The birds affected by the severe clinical form of disease (II B group, which were not treated with Seled and whose deficient feed was not corrected, could not recover and levels of sulfur-containing amino acids cysteine and methionine did not normalize.

  15. Immunoreactive cortisone in droppings reflect stress levels, diet and growth rate of gull-billed tern chicks.

    Science.gov (United States)

    Albano, Noelia; Santiago-Quesada, Francisco; Masero, José A; Sánchez-Guzmán, Juan M; Möstl, Erich

    2015-03-01

    Blood levels of corticosterone have been traditionally analyzed to assess stress levels in birds; however, measuring steroid hormone metabolites in feces and droppings has gained much interest as a noninvasive technique successfully used for such purposed in vertebrates. Diet may affect these fecal metabolite levels (e.g., due to nutritional stress), however, this variable has not been taken into account in studies with chicks despite the great dietary flexibility of many avian species. In this study, we addressed for the first time this key issue and validated the technique in wild gull-billed tern chicks (Gelochelidon nilotica). Several enzyme immunoassays were used to determine the most appropriate test to measure the stress response. Subsequently, we performed an experiment in captivity to assess adrenocortical activity in gull-billed tern chicks fed with two diets: piscivorous vs. insectivorous. Finally, the relation between the chicks' growth rate and excreted immunoreactive glucocorticoid metabolites (EGMs) was also evaluated. We found the immunoreactive cortisone metabolites to be a good index of stress (as being an index of adrenocortical reactivity) in chicks of this species. Fish-fed chicks had higher levels of cortisone metabolites when comparing both concentration and total daily excreted metabolites. Within each treatment diet, cortisone metabolite levels and growth rates were negatively correlated. These findings suggest that the diet should be considered when using this technique for comparative purposes and highlight the trade-off between stress levels and chicks growth rates. Copyright © 2015 Elsevier Inc. All rights reserved.

  16. Effect of dried solids of nejayote on broiler growth.

    Science.gov (United States)

    Velasco-Martinez, M; Angulo, O; Vazquez-Couturier, D L; Arroyo-Lara, A; Monroy-Rivera, J A

    1997-11-01

    The purpose of the present study was to test the suitability of the solids of nejayote (a waste product from the tortilla industry) in diets for broilers. The nejayote was obtained from two different tortilla-making factories and the solids were obtained by centrifuge then dried in a hot-air drier. Diets were formulated to be isocaloric and isonitrogenous according to the NRC dietary requirements (1994). Nejayote solids were supplemented at 2, 4, and 6% of the diet. Results show that the content of protein and calcium in the dried solids of nejayote were 5 and 13%, respectively. The performance of broilers fed diets supplemented with dried nejayote did not differ from that of those fed the control diet. Therefore, it is concluded that nejayote solids are suitable for broiler feed and do not affect growth performance. Utilization of nejayote solids at higher levels is a possibility provided that no adverse effects on body weight, feed utilization, and feed:gain ratios are observed.

  17. Response of broilers to feeding low-calcium and phosphorus diets plus phytase under different environmental conditions: body weight and tibiotarsus mineralization.

    Science.gov (United States)

    Catalá-Gregori, P; García, V; Hernández, F; Madrid, J; Cerón, J J

    2006-11-01

    Three experiments on Ross broiler chickens were conducted in 3 locations: cages (Experiment 1), floor pens (Experiment 2), and commercial farms (Experiment 3). The effect of low-total P (TP) wheat-soybean based diets plus microbial phytase (Natuphos) was evaluated. Four experimental starter and finisher diets were used in a 2-phase feeding program, as follows: control diet (SC until 21 d, FC from 22 to 42 d); 2 diets (SL400 and SL600 until 21 d, FL400 and FL600 from 22 to 42 d) with low TP (0.61% for starter and 0.54% for finisher), including 400 and 600 U/kg of phytase, respectively; and a very low-TP (0.52% for starter and 0.44% for finisher) diet (SVL600 until 21 d, FVL600 from 22 to 42 d) with 600 U/kg of phytase. In Experiment 1 (broilers in cages had movement limitation and no access to litter), no differences in BW, tibiotarsus mineralization, or mineral metabolism were observed among diets. In Experiment 2 (broilers in floor pens had movement limitation and access to litter), at 21 d of age, the lowest tibiotarsus ash percentage and BW were shown by birds fed the SVL600 diet. At 42 d of age, broilers fed the FC diet were the lightest. For the rest of the parameters of tibiotarsus mineralization and mineral metabolism measured in Experiment 2, no differences were shown. In Experiment 3 (broilers in commercial farms had access to litter without movement limitation), the BW of broilers fed the SC diet was the highest at 21 d of age. At 42 d of age, the broilers fed FL400 and FL600 diets were the heaviest. At the end of Experiment 3, broilers fed the FC diet had the highest dry litter Ca and P, whereas broilers fed the FVL600 diet had the lowest values. In conclusion, the very low-TP wheat-soybean based diet supplemented with 600 U/kg of phytase was sufficient to optimize all the parameters measured in Experiment 1 but not in Experiments 2 and 3. Therefore, when evaluating Ca and P in phytase-supplemented diets for broilers, it is necessary to bear in mind the

  18. The influence of the selection of macronutrients coupled with dietary energy density on the performance of broiler chickens.

    Science.gov (United States)

    Liu, Sonia Y; Chrystal, Peter V; Cowieson, Aaron J; Truong, Ha H; Moss, Amy F; Selle, Peter H

    2017-01-01

    A total of 360 male Ross 308 broiler chickens were used in a feeding study to assess the influence of macronutrients and energy density on feed intakes from 10 to 31 days post-hatch. The study comprised ten dietary treatments from five dietary combinations and two feeding approaches: sequential and choice feeding. The study included eight experimental diets and each dietary combination was made from three experimental diets. Choice fed birds selected between three diets in separate feed trays at the same time; whereas the three diets were offered to sequentially fed birds on an alternate basis during the experimental period. There were no differences between starch and protein intakes between choice and sequentially fed birds (P > 0.05) when broiler chickens selected between diets with different starch, protein and lipid concentrations. When broiler chickens selected between diets with different starch and protein but similar lipid concentrations, both sequentially and choice fed birds selected similar ratios of starch and protein intake (P > 0.05). However, when broiler chickens selected from diets with different protein and lipid but similar starch concentrations, choice fed birds had higher lipid intake (129 versus 118 g/bird, P = 0.027) and selected diets with lower protein concentrations (258 versus 281 g/kg, P = 0.042) than birds offered sequential diet options. Choice fed birds had greater intakes of the high energy diet (1471 g/bird, P macronutrients from 10-31 days in choice and sequential feeding groups were plotted and compared with the null path if broiler chickens selected equal amounts of the three diets in the combination. Regardless of feeding regimen, the intake paths of starch and protein are very close to the null path; however, lipid and protein intake paths in choice fed birds are father from the null path than sequentially fed birds.

  19. Growth of Audouin's gull chicks: the role of prehatch and posthatch factors

    Directory of Open Access Journals (Sweden)

    Elena Villuendas

    2003-07-01

    Full Text Available We compared the growth rates of Audouin´s Gull (Larus audouinii chicks from the Ebro Delta and Columbretes Islands. Chicks from the Columbretes Islands were reared in captivity and fed ad libitum. Wild chicks from the Ebro Delta weighed more than chicks hatched from Columbretes eggs but attained a lower weight at fledging. Chicks from Columbretes eggs hatched with a longer wing length but rates did not differ between samples during the early stages of growth. Eggs from the Ebro Delta were significantly larger than eggs from Columbretes. Hence, differences in growth appeared not to be related to differences in egg size (prehatch factor but only in parental quality (posthatch factor through the amount of food delivered to chicks. These differences could be due to the incapacity of parents to provide enough food during the last stages of chick development, in part because of reduced food availability at the Ebro Delta during the study period. Alternatively, differences could be attributed to a trade-off between present and future reproduction. Demographic consequences of reduced food availability for the 1993 Ebro Delta cohort should be explored in future work.

  20. Yellow mealworm larvae (Tenebrio molitor, L.) as a possible alternative to soybean meal in broiler diets.

    Science.gov (United States)

    Bovera, F; Piccolo, G; Gasco, L; Marono, S; Loponte, R; Vassalotti, G; Mastellone, V; Lombardi, P; Attia, Y A; Nizza, A

    2015-01-01

    The aim of the study was to evaluate the feasibility of replacing soybean meal (SBM) with Tenebrio molitor larvae (TML) meal in broiler diets. A total of 80 30-d-old male Shaver brown broilers were divided into two groups fed on two isoproteic and isoenergetic diets differing for protein source (SBM vs. TML). Up to 62 d of age, body weight and feed intake were recorded weekly and body weight gain, feed conversion ratio (FCR), protein efficiency ratio (PER) and European efficiency factor (EEF) were calculated. At 62 d, blood samples were collected from 16 birds/group for evaluation of blood profiles. Feed intake was not different between groups considering the entire period of the trial. The FCR was more favourable in the TML than SBM group from 46 d of age and in the entire period of the trial (4.13 vs. 3.62). The PER was higher in the SBM than in the TML group (1.92 vs. 1.37) while the EEF was higher in broilers fed on the TML diet (132.6 vs. 156.2). Albumin-to-globulin ratio was higher in broilers fed on SBM than in the other group (0.44 vs. 0.30). aspartate aminotransferase and alanine aminotransferase were higher in TML than SBM (195.1 vs. 178.6 U/l and 82.07 vs. 46.71 U/l, respectively). Uric acid was higher in broilers fed on SBM than TML (5.40 vs. 4.16 mg/dl). TML did not affect feed intake and growth rate of broilers from 30 to 62 d of age when compared to an isoproteic and isoenergetic SBM diet, but FCR of the TML group was more favourable than that of the SBM group. The lowest albumin-to-globulin ratio in broilers fed on TML suggests a higher immune response, probably due to the prebiotic effects of chitin.

  1. Evaluation of Emblica officinalis fruit powder as a growth promoter in commercial broiler chickens

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    A. P. Patel

    2016-02-01

    Full Text Available Aim: The present study was conducted to evaluate the dietary addition of Emblica officinalis (Amla fruit powder as a growth promoter in commercial broiler chickens. Materials and Methods: An experiment was conducted on 135 commercial broiler chicks (Ven-Cobb 400 strain divided into three groups with three replicates of 15 chicks each. Three treatment groups were as follows – T1: Basal diet as per BIS standards; T2: Basal diet supplemented with 0.4% of E. officinalis fruit powder; and T3: Basal diet supplemented with 0.8% of E. officinalis fruit powder. Results: The average body weights at the end of the 6th week were significantly higher (p<0.05 in groups T2 and T3 compared to group T1. Feed intake, feed conversion ratio and feed cost per kg live weight production were similar among the treatment groups. The net profit per bird was the highest in group T2 (Rs. 19.22/bird followed by group T3 (Rs. 17.86/bird and the lowest in group T1 (Rs. 14.61/bird. Conclusion: Based on the results of the present study, it was concluded that dietary addition of E. officinalis (Amla fruit powder had a positive effect on growth performance and net profit per bird in commercial broiler chickens.

  2. Experimental infection of broiler chicks with Salmonella Typhimurium from pigeon (Columba livia

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    Átilla Holanda de Albuquerque

    2016-08-01

    Full Text Available Several cases of animal and human salmonellosis caused by the Salmonella serotype Typhimurium have been reported. In animals, subclinical infection favors pathogen dissemination through feces. In this context, the domestic pigeon (Columba livia with an asymptomatic condition may play an important role in the transmission of salmonellosis, through the elimination of contaminated feces in commercial aviaries or in poultry feed facilities, causing economic losses to the poultry industry and presenting a risk to public health. This study aimed to evaluate the mortality, clinical signs and the presence of Salmonella Typhimurium in the feces and organs of chicks previously inoculated with bacteria isolated from a pigeon. One-day-old chicks were distributed in two experimental groups (G1 and G2 of 32 birds each, and a control group of six birds. Two inocula of 0.4 and 0.7 mL with 105 and 106 colony forming units were used in G1 and G2 birds, respectively. At 1, 4, 7 and 14 days post-inoculation (dpi fecal samples were pooled from each cage and individual cloacal swabs were collected. At 14 dpi, all chicks were euthanized and samples were collected from the liver, spleen, lung, cecum and intestine for microbiological analysis. Mortality was only observed among G2 birds (6.25%. Most birds presented clinical signs of diarrhea at 4 dpi and no symptom as observed at 14 dpi. The results from cloacal swabs demonstrated bacterial elimination in 68.8% and 53.1% of G2 and G1 birds, respectively at 1 dpi. Additionally, fecal samples had elevated bacterial shedding in all four periods of observation , with a higher excretion at 4 dpi (62.5% for both groups. Among G2 birds, 74.2% were positive for the pathogen in the intestine; G1 birds presented the lowest rate of lung infection (29%, and both groups had more than 50% positivity for liver and caeca. The results revealed that infected chicks with a Salmonella Typhimurium strains isolated from pigeons may host the

  3. Evaluating the suitability of maggot meal as a partial substitute of soya bean on the productive traits, digestibility indices and organoleptic properties of broiler meat.

    Science.gov (United States)

    Khan, S; Khan, R U; Sultan, A; Khan, M; Hayat, S U; Shahid, M S

    2016-08-01

    This study was conducted to replace soya bean meal with maggot meal at difference percentages. A total of 120 one-day-old broiler chicks were divided into four groups. Four diets were formulated with maggot meal to replace soya bean meal at the rate of 0%, 10%, 20% and 30% designated as A, B, C and D respectively. Feed and water were offered ad libitum during the study which lasted for 28 days. Feed intake decreased significantly in treated groups while body weight, Feed conversion ratio and dressing percentage increased significantly in group D. Apparent metabolizable energy increased significantly in the group fed 30% maggot meal; however, no significant difference was found in dry matter, crude protein, crude fibre, ether extract, ash and nitrogen free extract between the control and treated groups. The result of sensory evaluation revealed that tenderness and juiciness increased significantly in group C and B respectively. The colour and flavour were most accepted in group B and C. The results indicate that replacement of soya bean meal at the rate of 30% in broiler feed produced most favourable results. Journal of Animal Physiology and Animal Nutrition © 2016 Blackwell Verlag GmbH.

  4. Thesis Abstract Levels and forms of vitamin D in broilers diets.

    Science.gov (United States)

    Mesquita, F R; Silva, M I A; Bertechini, A G

    2016-05-09

    This study aimed to evaluate the concentration effects of two vitamin D isoforms, cholecalciferol (D3) and 25-hydroxycholecalciferol (25-OHD3) in broilers diets on performance, bone and physiological features of these birds. Of a total of 1920 one-day-old male chicks Cobb-500 were used from commercial hatchery, reared under bed creation systems. The animals were distributed in six treatments and eight replicates with 40 birds per treatment in a completely randomized design. The following vitamin D supplementation levels were applied: 70 and 87.5 μg/kg feed in initial phase; 56 and 70 μg/kg feed during the growth phase, and 35 and 47.35 μg/kg of feed in final phase of creation, obtained from two forms (D3 and 25-OHD3). The treatments consisted of supplementation of two levels from each isolated source and their associations (60% D3 + 40% 25-OHD3) according to the study phases. In the metabolism assay, 480 birds (14 and 35 days of age) were separated to be used for evaluation of calcium (Ca) and phosphorus (P) retention and excretion during the periods of 19 to 21 days and 40 to 42 days of age. The diets were based on corn and soybean meal, with supplementation of phytase (500 FTU/kg). The performance, bone characteristics, plasma levels, bone radiographic density, carcass yield, and P and Ca retention were evaluated. In the initial creation phase, we observed an increased P excretion by broilers fed diets supplemented with vitamin D3 (P < 0.05). In addition, the association between the two vitamin D isoforms resulted in higher retention of Ca and P than the birds fed diets supplemented only with vitamin D3 (P < 0.05), and higher P retention when compared to birds fed diets supplemented with 25-OHD3 (P < 0.05). Dietary supplemental 25-OHD3 at 87.5 μg/kg resulted in higher plasma levels of Ca in relation to the same supplemented source with 70 μg/kg at 21 days of age (P < 0.05). In the final phase, the birds fed diets supplemented with vitamin D3 presented the

  5. Effect of oral dietary supplement for chicks subjected to thermal oscillation on performance and intestinal morphometry

    Directory of Open Access Journals (Sweden)

    Jovanir Inês Müller Fernandes

    2017-09-01

    Full Text Available The aim of the study was to evaluate the efficacy of a nutritional formulation based on amino acids and vitamins supplemented in the drinking water for chicks in the first week of life subjected to thermal oscillation on performance, organ development and intestinal morphometry from 1 to 21 days. 640-male broiler chicks were distributed in a 2x2 factorial completely randomized design (with or without dietary supplementation and at comfort temperature or thermal oscillation. Chicks subjected to thermal oscillation presented worse performance (p < 0.05 than those under thermal comfort of 1 to 7, 1 to 14 and 1 to 21 days. Nutritional supplementation did not alter the performance (p < 0.05 of the birds, but resulted in a higher body weight (p < 0.05 regardless of the environmental thermal condition. At 7 days, chicks under thermal comfort had better intestinal morphometric parameters (p < 0.05, in relation to birds under thermal oscillation. In conclusion, the temperature oscillations caused negative consequences to the productive performance and the intestinal morphology of chicks for which dietary supplementation was not enough to mitigate the effects of the environmental challenge during the first week of life of the birds.

  6. Effects of feed supplemented with fermented pine needles (Pinus ponderosa) on growth performance and antioxidant status in broilers.

    Science.gov (United States)

    Wu, Q J; Wang, Z B; Wang, G Y; Li, Y X; Qi, Y X

    2015-06-01

    The objective of this study was to compare the effects of Aspergillus niger-fermented pine needles and nonfermented pine needles on growth performance and antioxidant capacity in broiler chicks. In total, 300 1-day-old broiler chicks were randomly allocated to 5 dietary treatments, which were then denoted as the control treatment (basal diet); the nonfermented treatment (containing 0.3% and 0.6% nonfermented treatment, respectively, in the starter and grower phase); or the fermented 1, fermented 2, or fermented 3 treatments. The fermented 1, fermented 2, and fermented 3 treatments contained 0.1, 0.3, and 0.5% fermented treatment, respectively, in the starter phase and 0.2, 0.6, and 1.0% fermented treatment, respectively, in the growth phase for 42 d. The results showed that fermentation treated supplementation had no adverse effect on the growth performance of broilers at 42 d of age. The activity of total nitric oxide synthase was significantly (P<0.05) decreased in the fermented treatment compared with the control and nonfermented treatments in broilers at 21 d of age. Compared with the control, broilers had higher (P<0.05) total superoxide dismutase activities and total antioxidant capacity when they were provided with either the fermented 2 or fermented 3 diet. The malondialdehyde content was significantly (P<0.05) decreased in the fermented 2 and fermented 3 treatments compared with the control and nonfermented treatments. It was concluded that the addition of fermented treatment to the diet could improve antioxidant capacity in broilers, as evidenced by the decrease in malondialdehyde and the increase in total superoxide dismutase activities; however, the effect of fermentation treatment on growth performance was negligible. © 2015 Poultry Science Association Inc.

  7. DIGESTIBLE VALINE REQUIREMENTS IN LOW-PROTEIN DIETS FOR BROILERS CHICKS

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    GR Nascimento

    Full Text Available ABSTRACT Three experiments were carried out to evaluate the levels of digestible valine in diets with reduced crude protein on the performance, carcass yield and muscle fiber diameter of male broilers during the pre-starter (1 to 8 d of age, starter (9 to 21 d of age and grower phases (21 to 42 d of age. A total of 1,080 chickens in the pre-starter phase, 900 in the starter phase and 864 in the grower phase were distributed in a completely randomized design with six treatments, consisting of a control positive diet (C+ and diets with 2 percentage points (p.p. reduction of crude protein level and five graded levels of digestible valine (Val, with six replicates of 30, 25 and 24 birds per experimental unit, respectively. The recommended level of TID Val in the low-protein diets for broilers in the pre-starter, starter and grower stages were 1.028; 0.905 and 0.789%, respectively. The reduction of 2 percentage points of the crude protein level in diets based on corn and soybean meal impaired (p ≤0.05 the feed conversion ratio in the starter and grower stages. Likewise, the reduced-protein diets decreased (p ≤0.05 muscle fiber diameter, but did not affect (p> 0.05 carcass and parts yields, or abdominal fat percentage at 42 days.

  8. Efficacy of supplemental natural zeolite in broiler chickens subjected to dietary calcium deficiency

    Directory of Open Access Journals (Sweden)

    Erol Bintaş

    2014-04-01

    Full Text Available Natural zeolite, or sodium aluminosilicate, influences calcium (Ca and phosphorus (P utilisation in chicks. A 2×2 factorial arrangement of treatments was used to investigate the effect of dietary Ca (recommended and below recommended levels and zeolite (0 and 0.8% on growth, plasma, tibia and faeces in chickens from 1 to 42 days of age. Zeolite supplementation did not affect overall body weight (BW gain, feed intake (FI or feed conversion ratio (FCR of broiler chickens (P>0.05. Overall mortality of zeolite-fed chickens was lower than in untreated ones (P<0.01. Reduction of dietary Ca of approximately 10 to 18% decreased (P<0.05 BW at 14 and 42 days of age in association with reduced FI, but overall FCR was unchanged. Serum protein and sodium constituents were reduced in birds fed zeolite (P<0.05. Decreasing dietary Ca level increased (P<0.01 serum, total protein and glucose concentrations, but decreased Ca level. Zeolite decreased bone ash in birds fed a Ca-deficient diet while increased faecal excretion of ash, Ca, P and aluminum. However, zeolite increased tibia weight (P<0.05 and thickness (P<0.01. No significant response (P>0.05 in relative weight and gross lesion scores of liver or footpad lesion scores was found related to changes in dietary regimens. The results of the present study do not corroborate the hypothesis that the effectiveness of zeolite may be improved in Ca-deficient diets in association with its ion exchange capability.

  9. Effects of rye inclusion in grower diets on immune competence-related parameters and performance in broilers

    NARCIS (Netherlands)

    Krimpen, van M.M.; Torki, M.; Schokker, D.

    2017-01-01

    An experiment was conducted to investigate the effects of dietary inclusion of rye, a model ingredient to increase gut viscosity, between 14 and 28 d of age on immune competence-related parameters and performance of broilers. A total of 960 day-old male Ross 308 chicks were weighed and randomly

  10. Daily rhythms of cloacal temperature in broiler chickens of different age groups administered with zinc gluconate and probiotic during the hot-dry season.

    Science.gov (United States)

    Aluwong, Tagang; Sumanu, Victory O; Ayo, Joseph O; Ocheja, Benjamin O; Zakari, Friday O; Minka, Ndazo S

    2017-06-01

    The aim of the experiment was to evaluate effects of zinc gluconate (ZnGlu) and probiotic administration on the daily rhythm of cloacal temperature ( t cloacal ) in broiler chickens of different age groups during the hot-dry season. One-day-old broiler chicks ( n  = 60) were divided into groups I-IV of 15 chicks per group, and treated for 35 days: Group I (control) was given deionized water; Group II, ZnGlu (50 mg/kg); Group III, probiotic (4.125 × 10 6  cfu/100 mL), and Group IV, ZnGlu (50 mg/kg) + probiotic (4.125 × 10 6  cfu/100 mL). Air dry-bulb temperature ( t db ), relative humidity (RH), and temperature-humidity index (THI) inside the pen, and t cloacal of each broiler chick were obtained bihourly over a 24-h period; on days 21, 28, and 35 of the study. Values of t db (32.10 ± 0.49°C), RH (49.94 ± 1.91%), and THI (38.85 ± 0.42) obtained were outside the thermoneutral zone for broiler chickens, and suggested that the birds were subjected to heat stress. Application of the periodic model showed disruption of daily rhythm of t cloacal in broilers on day 21, which was synchronized by probiotic administration. The administration of probiotics or ZnGlu + probiotics to a greater extent decreased the mesor and amplitude, delayed the acrophases of t cloacal in broilers, especially at day 35, as compared to the controls. Overall, the t cloacal values in broiler chickens administered with probiotic alone (41.25 ± 0.05°C) and ZnGlu + probiotic (41.52 ± 0.05°C) were lower ( P  probiotic alone synchronized t cloacal of the birds at day 21, and, in addition, decreased t cloacal response most, followed by its coadministration with ZnGlu, the antioxidants may be beneficial in modulating daily rhythmicity of t cloacal and alleviating adverse effects of heat stress on broiler chickens during the hot-dry season. © 2017 The Authors. Physiological Reports published by Wiley Periodicals, Inc. on behalf of The Physiological Society and the

  11. Effects of dietary coenzyme Q10 supplementation on hepatic mitochondrial function and the activities of respiratory chain-related enzymes in ascitic broiler chickens.

    Science.gov (United States)

    Geng, A L; Guo, Y M

    2005-10-01

    1. One hundred and sixty 1-d-old Arbor Acre male broiler chicks were fed with maize-soybean based diets for 6 weeks in a 2 x 2 factorial experiment. The factors were CoQ10 supplementation (0 or 40 mg/kg) and Escherichia coli lipopolysaccharide (LPS) challenge (LPS or saline). 2. CoQ10 was supplemented from d 1. From d 18, the chickens received three weekly i.p. injections of LPS (1.0 mg/kg BW) or an equivalent amount of sterile saline as control. From d 10 on, all chickens were exposed to low ambient temperature (12 to 15 degrees C) to induce ascites. 3. The blood packed cell volume and ascites heart index of broiler chickens were reduced by dietary CoQ10 supplementation. Mitochondrial State 3 and State 4 respiration, respiratory control ratio and phosphate oxygen ratio were not changed, but H+/site stoichiometry of complex II + III was elevated by dietary CoQ10 supplementation. 4. Cytochrome c oxidase and H+-ATPase activity were increased by CoQ10 supplementation, whereas NADH cytochrome c reductase and succinate cytochrome c reductase were not influenced. Mitochondrial anti-ROS capability was increased and malondialdehyde content was decreased by CoQ10 supplementation. 5. The work suggested that dietary CoQ10 supplementation could reduce broiler chickens' susceptibility to ascites, which might be the result of improving hepatic mitochondrial function, some respiratory chain-related enzymes activities and mitochondrial antioxidative capability.

  12. Leaky gut and mycotoxins: Aflatoxin B1 does not increase gut permeability in broiler chickens

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    Rosario eGalarza-Seeber

    2016-02-01

    Full Text Available Previous studies conducted in our laboratory have demonstrated that intestinal barrier function can be adversely affected by diet ingredients or feed restriction, resulting in increased intestinal inflammation-associated permeability. Two experiments were conducted in broilers to evaluate the effect of 3 concentrations of Aflatoxin B1 (AFB1; 2, 1.5 or 1 ppm on gastrointestinal leakage and liver bacterial translocation (BT. In Exp 1, 240 day-of-hatch male broilers were allocated in two groups, each group had six replicates of 20 chickens (n = 120/group: Control feed or feed + 2 ppm AFB1. In Exp 2, 240 day-of-hatch male broilers were allocated in three groups, each group had 5 replicates of 16 chickens (n = 80/group: Control feed; feed + 1 ppm AFB1; or feed + 1.5 ppm AFB1. In both experiments, chickens were fed starter (d1-d7 and grower diets (d8-d21 ad libitum and performance parameters were evaluated every week. At day 21, all chicks received an oral gavage dose of FITC-d (4.16 mg/kg 2.5h before collecting blood samples to evaluate gastrointestinal leakage of FITC-d. In Exp 2 a hematologic analysis was also performed. Liver sections were aseptically collected and cultured using TSA plates to determine BT. Cecal contents were collected to determine total cfu/g of Gram-negative bacteria; lactic acid bacteria (LAB or anaerobes by plating on selective media. In Exp 2, liver, spleen and bursa of Fabricius were removed to determine organ weight ratio, and also intestinal samples were obtained for morphometric analysis. Performance parameters, organ weight ratio and morphometric measurements were significantly different between control and AFB1 groups in both experiments. Gut leakage of FITC-d was not affected by the three concentrations of AFB1 evaluated (P > 0.05. Interestingly, a significant reduction in BT was observed in chickens that received 2 and 1 ppm AFB1. An increase (P < 0.05 in total aerobic bacteria, total Gram negatives, and total LAB

  13. The influence of the selection of macronutrients coupled with dietary energy density on the performance of broiler chickens.

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    Sonia Y Liu

    Full Text Available A total of 360 male Ross 308 broiler chickens were used in a feeding study to assess the influence of macronutrients and energy density on feed intakes from 10 to 31 days post-hatch. The study comprised ten dietary treatments from five dietary combinations and two feeding approaches: sequential and choice feeding. The study included eight experimental diets and each dietary combination was made from three experimental diets. Choice fed birds selected between three diets in separate feed trays at the same time; whereas the three diets were offered to sequentially fed birds on an alternate basis during the experimental period. There were no differences between starch and protein intakes between choice and sequentially fed birds (P > 0.05 when broiler chickens selected between diets with different starch, protein and lipid concentrations. When broiler chickens selected between diets with different starch and protein but similar lipid concentrations, both sequentially and choice fed birds selected similar ratios of starch and protein intake (P > 0.05. However, when broiler chickens selected from diets with different protein and lipid but similar starch concentrations, choice fed birds had higher lipid intake (129 versus 118 g/bird, P = 0.027 and selected diets with lower protein concentrations (258 versus 281 g/kg, P = 0.042 than birds offered sequential diet options. Choice fed birds had greater intakes of the high energy diet (1471 g/bird, P < 0.0001 than low energy (197 g/bird or medium energy diets (663 g/bird whilst broiler chickens were offered diets with different energy densities but high crude protein (300 g/kg or digestible lysine (17.5 g/kg concentrations. Choice fed birds had lower FCR (1.217 versus 1.327 g/g, P < 0.0001 and higher carcass yield (88.1 versus 87.3%, P = 0.012 than sequentially fed birds. This suggests that the dietary balance between protein and energy is essential for optimal feed conversion efficiency. The intake path

  14. Effect of melatonin and lighting schedule on energy metabolism in broiler chickens

    NARCIS (Netherlands)

    Apeldoorn, E.J.; Schrama, J.W.; Mashaly, M.M.; Parmentier, H.K.

    1999-01-01

    The effect of melatonin and lighting schedule on energy metabolism in broiler chickens was studied. Eight groups of six female broiler chickens each were assigned to a continuous lighting schedule [23 h light (L):1 h darkness (D)] or an intermittent lighting schedule (1L:3D), and were fed a diet

  15. Anticoccidial activity of Curcuma longa L. in broilers

    OpenAIRE

    Abbas,Rao Zahid; Iqbal,Zafar; Khan,Muhammad Nisar; Zafar,Muhammad Arif; Zia,Muhammad Anjum

    2010-01-01

    Comparative efficacy of turmeric (Curcuma longa L.) crude powder and salinomycin sodium on the occurrence of coccidiosis and growth performance of broiler was evaluated. A total of 90, day-old chicks were randomly divided into six groups. From first day onward, ration was supplemented with 1, 2 and 3 % turmeric powder in groups 2, 3 and 4, respectively, group 1 received salinomycin sodium @ 12 g 50 kg-1 feed while groups 5 and 6 were kept as infected un-medicated and uninfected un-medicated c...

  16. The effect of guar meal (germ fraction) and β- mannanase enzyme ...

    African Journals Online (AJOL)

    REZVAN

    2012-05-01

    May 1, 2012 ... A completely randomized design experiment was conducted to assess the effect of β-mannanase enzyme on a commercial broiler chickens strain fed with different levels of guar meal germ fraction. (GM) Each treatment was assigned to 4 replicate for a total of 24 pens, consisting of 15 chicks per pen,.

  17. The impact of nutrient density in terms of energy and/or protein on live performance, metabolism and carcass composition of female and male broiler chickens of two commercial broiler strains.

    Science.gov (United States)

    Delezie, E; Bruggeman, V; Swennen, Q; Decuypere, E; Huyghebaert, G

    2010-08-01

    The objective of this study was to investigate the effects of diet composition on performance, slaughter yield and plasma metabolites, as different modern broiler strains show different responses to feed intake. Broilers of two commercial strains and of both sexes received one of three diets being different in energy and/or protein level [control diet, low energy/low protein diet (LM/LP) and low protein diet (LP)]. Low energy/low protein diet chickens were characterized by significantly lower body weights and feed intake compared with their LP and control counterparts. Broilers of the Cobb strain or broilers that were fed the control diet were most efficient in converting energy to body weight. No significant differences in plasma metabolites were detected due to diet composition or genotype. The diet with the lower energy and crude protein levels reached the lowest slaughter yield but the highest drumstick and wing percentages. The lowest mortality percentages were observed for broilers fed the LM/LP diet, and Cobb birds appeared to be more sensitive for metabolic disorders resulting in death. It is obvious from this study that different genotypes respond differently to changes in diet composition and therefore have adjusted nutritional requirements.

  18. Noni (Morinda citrifolia) Modulates the Hypothalamic Expression of Stress- and Metabolic-Related Genes in Broilers Exposed to Acute Heat Stress.

    Science.gov (United States)

    Rajaei-Sharifabadi, Hossein; Ellestad, Laura; Porter, Tom; Donoghue, Annie; Bottje, Walter G; Dridi, Sami

    2017-01-01

    Heat stress (HS) adversely affects growth performance and inflicts heavy economic losses to the poultry industry. There is, therefore, a critical need to identify new alternative strategies to alleviate the negative effects induced by HS. The tropic medicinal plant, Morinda citrifolia (Noni), is being used in livestock nutrition, however the literature is limited and conflicting for its impact on growth performance. The present study aimed to determine the effect of Noni on feeding and drinking behavior as well as on the hypothalamic expression of stress- and metabolic-related genes in broiler chickens exposed to acute HS. A total of 480 1 day-old male broiler chicks were randomly assigned to 12 controlled environmental chambers. Birds were subjected to two environmental conditions (TN, 25°C vs. HS, 35°C for 2 h) and fed two diets (control vs. 0.2% Noni) in a 2 × 2 factorial design. Feed intake and core body temperature (BT) were recorded during HS period. Blood was collected and hypothalamic tissues were harvested for target gene and protein analyses. Acute HS-broilers exhibited higher BT (~1°C), spent less time eating with a significant decrease in feed intake, and spent more time drinking along with higher drinking frequency compared to those maintained under TN conditions. Although Noni supplementation did not improve feed intake, it significantly delayed (~30 min) and reduced the BT-induced by HS. At molecular levels and under HS conditions, Noni supplementation down regulated the hypothalamic expression of HSP90 and its related transcription factors HSF1, 2, and 4, increased orexin mRNA levels, and decreased the phosphorylation levels of AMPKα1/2 Thr172 and mTOR Ser2481 . Together, these data indicated that Noni supplementation might modulate HS response in broilers through central orexin-AMPK-mTOR pathways.

  19. Noni (Morinda citrifolia Modulates the Hypothalamic Expression of Stress- and Metabolic-Related Genes in Broilers Exposed to Acute Heat Stress

    Directory of Open Access Journals (Sweden)

    Hossein Rajaei-Sharifabadi

    2017-12-01

    Full Text Available Heat stress (HS adversely affects growth performance and inflicts heavy economic losses to the poultry industry. There is, therefore, a critical need to identify new alternative strategies to alleviate the negative effects induced by HS. The tropic medicinal plant, Morinda citrifolia (Noni, is being used in livestock nutrition, however the literature is limited and conflicting for its impact on growth performance. The present study aimed to determine the effect of Noni on feeding and drinking behavior as well as on the hypothalamic expression of stress- and metabolic-related genes in broiler chickens exposed to acute HS. A total of 480 1 day-old male broiler chicks were randomly assigned to 12 controlled environmental chambers. Birds were subjected to two environmental conditions (TN, 25°C vs. HS, 35°C for 2 h and fed two diets (control vs. 0.2% Noni in a 2 × 2 factorial design. Feed intake and core body temperature (BT were recorded during HS period. Blood was collected and hypothalamic tissues were harvested for target gene and protein analyses. Acute HS-broilers exhibited higher BT (~1°C, spent less time eating with a significant decrease in feed intake, and spent more time drinking along with higher drinking frequency compared to those maintained under TN conditions. Although Noni supplementation did not improve feed intake, it significantly delayed (~30 min and reduced the BT-induced by HS. At molecular levels and under HS conditions, Noni supplementation down regulated the hypothalamic expression of HSP90 and its related transcription factors HSF1, 2, and 4, increased orexin mRNA levels, and decreased the phosphorylation levels of AMPKα1/2Thr172 and mTORSer2481. Together, these data indicated that Noni supplementation might modulate HS response in broilers through central orexin-AMPK-mTOR pathways.

  20. Tolerance and safety evaluation of N,N-dimethylglycine, a naturally occurring organic compound, as a feed additive in broiler diets.

    Science.gov (United States)

    Kalmar, Isabelle D; Verstegen, Martin W A; Maenner, Klaus; Zentek, Jurgen; Meulemans, Godelieve; Janssens, Geert P J

    2012-06-01

    N,N-Dimethylglycine (DMG) is a tertiary amino acid that naturally occurs as an intermediate metabolite in choline-to-glycine metabolism. The objective of the present trial was to evaluate tolerance, safety and bioaccumulation of dietary DMG in broilers when supplemented at 1 g and 10 g Na-DMG/kg. A feeding trial was conducted using 480 1-d-old broiler chicks that were randomly allocated to twenty-four pens and fed one of three test diets added with 0, 1 or 10 g Na-DMG/kg during a 39 d growth period. Production performance was recorded to assess tolerance and efficacy of the supplement. At the end of the trial, toxicity was evaluated by means of haematology, plasma biochemistry and histopathology of liver, kidney and heart (n 12), whereas bioaccumulation was assessed on breast meat, liver, blood, kidney and adipose tissue (n 8). Carcass traits were similar between the control and 1 g Na-DMG/kg feed groups (P>0·05), but the feed:gain ratio was significantly improved at 1 g Na-DMG/kg feed compared with the control or the 10-fold dose (P=0·008). Histological examinations showed no pathological effects and results of haematology and plasma biochemistry revealed similar values between the test groups (P>0·05). Bioaccumulation occurred at the 10-fold dose, but the resulting DMG content in breast meat was comparable with, for instance, wheat bran and much lower than uncooked spinach. In conclusion, DMG at 1 g Na-DMG/kg improved the feed:gain ratio in broilers without DMG being accumulated in consumer parts. Furthermore, dietary supplementation with DMG up to 10 g Na-DMG/kg did not induce toxicity or impaired performance in broilers.