WorldWideScience

Sample records for broadcast-sown oilseed rape

  1. Determinants of nectar production in oilseed rape

    DEFF Research Database (Denmark)

    Enkegaard, Annie; Kryger, Per; Boelt, Birte

    2016-01-01

    With the aim of contributing to the development of models forecasting the melliferous characteristics and the potential honey yield of oilseed rape, we analyzed data on the temporal pattern of number of oilseed rape flowers, nectar secretion, sugar concentration, and weight of bee hives in relation...... with significantly reduced quality of the nectar in terms of both volume and sugar concentration, and our results suggest that the oilseed rape in this year did not contribute notably to bee colony development. In years in which the oilseed rape flowered around the normal time (beginning of May), the amount...... of secreted sugar decreased over the flowering period, being governed by inherent oilseed rape characteristics modified by climatic factors preceding the onset of flowering, as well as climatic conditions during flowering. The exploitation of oilseed rape nectar by bee colonies was found to be influenced...

  2. You reap what you sow - or do you? volunteers in organic row-sown and broadcast-sown oilseed rape fields

    DEFF Research Database (Denmark)

    Andersen, Naja Steen; Rasmussen, Jesper; Bagger Jørgensen, Rikke

    2010-01-01

    volunteers would exceed the threshold of 0.9%, and thus GM labelling would be demanded. Some of the volunteers belonged to varieties cultivated 8–11 years previously, indicating a long persistence of volunteer populations. What you reap might be added in the past – with consequences for organic farmers......’ production and earning....

  3. Bacillus megaterium A6 suppresses Sclerotinia sclerotiorum on oilseed rape in the field and promotes oilseed rape growth

    Science.gov (United States)

    Sclerotinia sclerotiorum causes serious yield losses in crops in The People’s Republic of China and other regions of the world. Two formulations of oilseed rape seed containing the plant-growth promoting bacterium Bacillus megaterium A6 were evaluated for suppression of this pathogen on oilseed rap...

  4. NMR metabolomics of ripened and developing oilseed rape (Brassica napus) and turnip rape (Brassica rapa).

    Science.gov (United States)

    Kortesniemi, Maaria; Vuorinen, Anssi L; Sinkkonen, Jari; Yang, Baoru; Rajala, Ari; Kallio, Heikki

    2015-04-01

    The oilseeds of the commercially important oilseed rape (Brassica napus) and turnip rape (Brassica rapa) were investigated with (1)H NMR metabolomics. The compositions of ripened (cultivated in field trials) and developing seeds (cultivated in controlled conditions) were compared in multivariate models using principal component analysis (PCA), partial least squares discriminant analysis (PLS-DA), and orthogonal partial least squares discriminant analysis (OPLS-DA). Differences in the major lipids and the minor metabolites between the two species were found. A higher content of polyunsaturated fatty acids and sucrose were observed in turnip rape, while the overall oil content and sinapine levels were higher in oilseed rape. The genotype traits were negligible compared to the effect of the growing site and concomitant conditions on the oilseed metabolome. This study demonstrates the applicability of NMR-based analysis in determining the species, geographical origin, developmental stage, and quality of oilseed Brassicas. Copyright © 2014 Elsevier Ltd. All rights reserved.

  5. Sensitization to turnip rape and oilseed rape in children with atopic dermatitis: a case-control study.

    Science.gov (United States)

    Poikonen, Sanna; Puumalainen, Tuija J; Kautiainen, Hannu; Palosuo, Timo; Reunala, Timo; Turjanmaa, Kristiina

    2008-08-01

    Turnip rape and oilseed rape 2S albumins are new allergens in children with atopic dermatitis suspected for food allergy. We recently found that 11% (206/1887) of these children had a positive skin prick test to seeds of oilseed rape (Brassica napus) and/or turnip rape (Brassica rapa). In the present case-control study we examined how the children with atopic dermatitis sensitized to turnip rape and oilseed rape had been breast-fed and whether they had some common sensitization pattern to certain foods or pollens. A total of 64 children with atopic dermatitis and a positive skin prick test to turnip rape and/or oilseed rape (>or=5 mm) were examined. Sixty-four age- and sex-matched children with atopic dermatitis but negative skin prick tests to turnip rape and oilseed rape served as case controls. The turnip rape and/or oilseed rape sensitized children with atopic dermatitis had significantly more often positive skin prick tests reactions and IgE antibodies to various foods (cow's milk, egg, wheat, mustard; p children. They had been exclusively breast-fed for a longer period (median 4 months; p Children with atopic dermatitis sensitized to oilseed rape and turnip rape had high frequency of associated sensitizations to all foods and pollens tested showing that oilseed plant sensitization affects especially atopic children who have been sensitized to multiple allergens.

  6. Oilseed rape genotypes response to boron toxicity

    Directory of Open Access Journals (Sweden)

    Savić Jasna

    2013-01-01

    Full Text Available Response of 16 oilseed rape genotypes to B (boron toxicity was analyzed by comparing the results of two experiments conducted in a glasshouse. In Experiment 1 plants were grown in standard nutrient solutions with 10 µMB (control and 1000 µM B. Relative root and shoot growth varied from 20-120% and 31-117%, respectively. Variation in B concentration in shoots was also wide (206.5-441.7 µg B g-1 DW as well as total B uptake by plant (62.3-281.2 µg B g1. Four selected genotypes were grown in Experiment 2 in pots filled with high B soil (8 kg ha-1 B; B8. Shoot growth was not affected by B8 treatment, while root and shoot B concentration was significantly increased compared to control. Genotypes Panther and Pronto which performed low relative root and shoot growth and high B accumulation in plants in Experiment 1, had good growth in B8 treatment. In Experiment 2 genotype NS-L-7 had significantly lower B concentration in shots under treatment B8, but also very high B accumulation in Experiment 1. In addition, cluster analyses classified genotypes in three groups according to traits contrasting in their significance for analyzing response to B toxicity. The first group included four varieties based on their shared characteristics that have small value for the relative growth of roots and shoots and large values of B concentration in shoot. In the second largest group were connected ten genotypes that are heterogeneous in traits and do not stand out on any characteristic. Genotypes NS-L-7 and Navajo were separated in the third group because they had big relative growth of root and shoot, but also a high concentration of B in the shoot, and high total B uptake. Results showed that none of tested genotypes could not be recommended for breeding process to tolerance for B toxicity. [Projekat Ministarstva nauke Republike Srbije, br. OI 173028

  7. Biological control of Sclerotinia sclerotiorum (oilseed rape isolate ...

    African Journals Online (AJOL)

    Sclerotinia sclerotiorum (Lib) De Bary, the causal agent of stem rot of oilseed rape, is one of the most important phytopathogens. In order to find appropriate biocontrol agents, antagonistic and especially chitinolytical activities of 110 soil actinomycetes were examined. Among assayed isolates, Streptomyces sp. isolate 422 ...

  8. Ethanol production from rape straw: Part of an oilseed rape biorefinery

    DEFF Research Database (Denmark)

    Arvaniti, Efthalia

    The aim of this study was 1) present an oilseed rape whole crop biorefinery; 2) to investigate the best available experimental conditions for production of cellulosic ethanol from rape straw, and included the processes of thermo-chemical pretreatment, enzymatic hydrolysis, and C6 fermentation......, and 3) to couple cellulosic ethanol production to production of cellulolytic enzymes that are needed for cellulosic ethanol production, inside a rape straw biorefinery. For the first is based less on available experiments, and more on literature review. The second and third study conclusions were drawn...... rapeseed biodiesel plant of Europe to an oilseed rape whole-crop biorefinery by 2020 is envisioned and discussed. The description and discussion of this biorefinery is based partly on literature review, and partly on own experimental data, especially on pretreatment of rape straw, and production...

  9. Evaluation of Yield and Yield Components of Oilseed Rape in the Wheat-Oilseed Rape Strip Intercropping Influenced by Chemical and Biological Fertilizers

    Directory of Open Access Journals (Sweden)

    R Amirmardfar

    2015-01-01

    Full Text Available To evaluate the effects of wheat (Triticum aestivum and oilseed rape (Brassica napus strip intercropping on yield components, seed and biological yields of oilseed rape, field experiments were carried out as factorial based on randomized complete block design with three replications at Research Farm of Tabriz University, Tabriz, Iran during 2010-2012 cropping seasons. The first factor consisted of four types of wheat and oilseed rape cropping system, sole crop of oilseed rape (A1,: strip intercropping with 8:3 (A2, 12:4 (A3 and 16:5 (A4 of wheat and oilseed rape rows, respectively and the other factor consisted of two fertilizer levels, B1: 100% chemical fertilizers (urea and triple superphosphate and B2: 50% chemical fertilizers + biofertilizers (Nitrazhin and Barvar2. The results showed that strip intercropping of wheat- oilseed rape resulted in significant increase in yield components, seed yield per occupied unit area and biological yield per occupied unit area of oilseed rape as compared with mono-cropping. The number of silique per plant in intercropping systems was significantly higher than that of mono-cropping. The highest seed yield was obtained in the 16:5 rows of wheat-oilseed rape with 343.76 g.m-2 and the lowest mean was observed in mono-cropping of oilseed rape with 260.21 g.m-2. Biological yield per occupied unit area and seed yield per intercropped unit area in B1 were significantly greater than that of B2, but this treatment had no significant effect on the other traits. Because, B1 and B2 had no significant difference in seed yield per occupied unit area and due to the importance of reduction in chemical fertilizers consumption and food and environmental health care, strip intercropping of wheat-oilseed rape under 50% chemical fertilizers + biofertilizers can be recommended as a suitable cultural method.

  10. Seed dressings to control slug damage in oilseed rape.

    Science.gov (United States)

    Simms, Louise C; Mullins, Christopher E; Wilson, Michael J

    2002-07-01

    Slugs are major pests of oilseed rape that are poorly controlled by conventional bait pellets. A series of laboratory experiments investigated the potential of seed-dressings to control slug damage in this crop. Four compounds: metaldehyde, methiocarb, cinnamamide and 3,5-dimethoxycinnamic acid (DMCA) were tested at a range of doses for phytotoxicity and ability to reduce damage by Deroceras reticulatum (Müller). Metaldehyde and methiocarb were not phytotoxic at any doses, whereas all doses of cinnamamide and DMCA were. All compounds reduced slug damage, but metaldehyde and methiocarb consistently performed better than cinnamamide and DMCA. Metaldehyde and methiocarb seed-dressings were compared with baited pellets containing the same active ingredients at recommended field doses. The seed-dressings protected plants from damage by D reticulatum and Arion subfuscus (Draparnaud) as well as, or better than, baited pellets. We therefore recommend that metaldehyde and methiocarb should be field-tested as seed dressings to control slugs in oilseed rape.

  11. Integrated pests monitoring of oilseed rape in means of biodiesel manufacture

    OpenAIRE

    Alasić, Vesna

    2008-01-01

    Because of its high oil content (35 - 44 %) oilseed rape is an important industrial culture. Recently, biodiesel manufacture is especially active. In fact, in the countries of the EU, manufacture of biodiesel has reached its optimum, but sadly, in Republic of Croatia that small amount of biodiesel that is manufactured is obtained from recycled oil. This paper writes about the statistic data in manufacture of oilseed rape, pests of oilseed rape and about presentation of the project: “Integrate...

  12. Endogaeic ground beetles fauna in oilseed rape field in Croatia

    Directory of Open Access Journals (Sweden)

    Zrinka DRMIĆ

    2016-09-01

    Full Text Available The abundance of ground beetles (Coleoptera, Carabidae in oilseed rape field can be an important indicator of different agricultural measures in arable land fauna. Surveys about ground beetle fauna in Croatia are generally very scarce and only few research on oilseed rape were conducted. The aim of this study was to determine the endogaeic ground beetle fauna in oilseed rape field and to determine species abundance and frequency. The study was carried out in Podravina region of Croatia, from the end of May till the mid-September in 2015. Ground beetles were collected using endogaeic traps incorporated in the soil. Altogether, 487 individuals classified into 8 species were collected. Species with the highest abundance and frequency was Brachinus (Brachinus psophia Audinet-Serville, 1821, which was classified as eudominant and constant species. The highest ground beetle abundance was observed in summer period. All of the species found were spring breeders, except for species Trechus (Trechus quadristriatus (Schrank, 1781 which breeds in autumn.

  13. Effects of NaCl treatment on the antioxidant enzymes of oilseed rape ...

    African Journals Online (AJOL)

    The effects of NaCl treatment on the activity of antioxidant enzymes in leaves of oilseed rape seedlings (Brassica napus L.) were studied. The results showed that the relative water content from leaves of oilseed rape seedlings was gradually decreased and the electronic conductivity was increased during 0 - 24 h under 200 ...

  14. Biological control of Sclerotinia disease by Aspergillus sp. on oilseed rape in the field

    Science.gov (United States)

    Sclerotinia sclerotiorum causes serious yield losses on oilseed rape and other crops worldwide. Aspergillus sp. Asp-4, previously shown to inhibit germination of sclerotia of S. sclerotiorum in vitro and in the field, was evaluated in field trials for suppression of this pathogen on oilseed rape. S...

  15. Effects of NaCl treatment on the antioxidant enzymes of oilseed rape ...

    African Journals Online (AJOL)

    STORAGESEVER

    2009-10-19

    Oct 19, 2009 ... The effects of NaCl treatment on the activity of antioxidant enzymes in leaves of oilseed rape seedlings. (Brassica napus L.) were studied. The results showed that the relative water content from leaves of oilseed rape seedlings was gradually decreased and the electronic conductivity was increased during 0.

  16. Formulations of Bacillus subtilis BY-2 suppress Sclerotinia sclerotiorum on oilseed rape in the field

    Science.gov (United States)

    We are developing a collection of Bacillus strains, isolated from different environments, for use in controlling Sclerotinia sclerotiorum on oilseed rape in China and elsewhere. Strain BY-2, isolated from internal tissues of an oilseed rape root, was demonstrated to be Bacillus subtilis based on bi...

  17. Are pesticide residues in honey related to oilseed rape treatments?

    Science.gov (United States)

    Karise, Reet; Raimets, Risto; Bartkevics, Vadims; Pugajeva, Iveta; Pihlik, Priit; Keres, Indrek; Williams, Ingrid H; Viinalass, Haldja; Mänd, Marika

    2017-12-01

    Pesticide treatments before and during the flowering of honey bee forage crops may lead to residues in honey. In northern regions oilseed rape belongs to the main forage crops that is mostly cultivated by means of intensive agriculture, including several pesticide treatments. However, in addition to the focal forage crops, pesticides from non-forage crops can spread to wild flowers around fields, and thus the residues in honey would reflect the whole range of pesticides used in the agricultural landscape. The aim of our study was to clarify which currently used pesticides are present in honey gathered from heterogeneous agricultural landscapes after the end of flowering of oilseed crops. Honey samples (N = 33) were collected from beehives of Estonia during 2013 and 2014, and analysed for residues of 47 currently used agricultural pesticides using the multiresidue method with HPLC-MS/MS and GC-MS and a single residue method for glyphosate, aminopyralid and clopyralid. Residues of eight different active ingredients with representatives from all three basic pesticide classes were determined. Although no correlation was detected between the cumulative amount of pesticide residues and percent of oilseed crops in the foraging territory, most of the residues are those allowed for oilseed rape treatments. Among all pesticides, herbicide residues prevailed in 2013 but not in 2014. Despite the relatively small agricultural impact of Estonia, the detected levels of pesticide residues sometimes exceeded maximum residue level; however, these concentrations do not pose a health risk to consumers, also acute toxicity to honey bees would be very unlikely. Copyright © 2017 Elsevier Ltd. All rights reserved.

  18. Components of a Rice-Oilseed Rape Production System Augmented with Trichoderma sp. Tri-1 Control Sclerotinia sclerotiorum on Oilseed Rape.

    Science.gov (United States)

    Hu, Xiaojia; Roberts, Daniel P; Xie, Lihua; Maul, Jude E; Yu, Changbing; Li, Yinshui; Zhang, Yinbo; Qin, Lu; Liao, Xing

    2015-10-01

    Sclerotinia sclerotiorum causes serious yield losses on many crops throughout the world. A multicomponent treatment that consisted of the residual rice straw remaining after rice harvest and Trichoderma sp. Tri-1 (Tri-1) formulated with the oilseed rape seedcake fertilizer was used in field soil infested with S. sclerotiorum. This treatment resulted in oilseed rape seed yield that was significantly greater than the nontreated control or when the fungicide carbendizem was used in the presence of this pathogen in field trials. Yield data suggested that the rice straw, oilseed rape seedcake, and Tri-1 components of this treatment all contributed incrementally. Similar treatment results were obtained regarding reduction in disease incidence. Slight improvements in yield and disease incidence were detected when this multicomponent treatment was combined with a fungicide spray. Inhibition of sclerotial germination by this multicomponent treatment trended greater than the nontreated control at 90, 120, and 150 days in field studies but was not significantly different from this control. This multicomponent treatment resulted in increased yield relative to the nontreated control in the absence of pathogen in a greenhouse pot study, while the straw alone and the straw plus oilseed rape seedcake treatments did not; suggesting that Tri-1 was capable of promoting growth. Experiments reported here indicate that a treatment containing components of a rice-oilseed rape production system augmented with Tri-1 can control S. sclerotiorum on oilseed rape, be used in integrated strategies containing fungicide sprays for control of this pathogen, and promote plant growth.

  19. Hormones and Pod Development in Oilseed Rape (Brassica napus) 1

    Science.gov (United States)

    de Bouille, Pierre; Sotta, Bruno; Miginiac, Emile; Merrien, André

    1989-01-01

    The endogenous levels of several plant growth substances (indole acetic acid, IAA; abscisic acid, ABA; zeatin, Z; zeatin riboside, [9R]Z; isopentenyladenine, iP; and isopentenyladenosine, [9R]iP were measured during pod development of field grown oilseed Rape (Brassica napus L. var oleifera cv Bienvenu) with high performance liquid chromatography and immunoenzymic (enzyme-linked immunosorbent assay, ELISA) techniques. Results show that pod development is characterized by high levels of Z and [9R]Z in 3 day old fruits and of IAA on the fourth day. During pod maturation, initially a significant increase of IAA and cytokinins was observed, followed by a progressive rise of ABA levels and a concomitant decline of IAA and cytokinin (except iP) levels. The relationship between hormone levels and development, especially pod number, seed number per pod, and seed weight determination, will be discussed. PMID:16666891

  20. Forecasting the Adoption of GM Oilseed Rape: Evidence from a Discrete Choice Experiment

    OpenAIRE

    Breustedt, Gunnar; Muller-Scheessel, Jorg; Latacz-Lohmann, Uwe

    2008-01-01

    This paper explores farmers’ willingness to adopt genetically modified oilseed rape prior to its commercial release and estimates the ‘demand’ for the new technology. The analysis is based upon choice experiments with 202 German arable farmers. A multinomial probit estimation revealed that GM attributes such as gross margin, expected liability from cross pollination, or flexibility in returning to conventional oilseed rape significantly affect the likelihood of adoption. Neighbouring farmers’...

  1. Mapping of QTL for seed dormancy in a winter oilseed rape doubled haploid population.

    Science.gov (United States)

    Schatzki, Jörg; Schoo, Burkhard; Ecke, Wolfgang; Herrfurth, Cornelia; Feussner, Ivo; Becker, Heiko C; Möllers, Christian

    2013-09-01

    Following winter oilseed rape cultivation, considerable numbers of volunteer oilseed rape plants may occur in subsequent years in following crops. The appearance of volunteer oilseed rape plants is based on the capability of the seeds to become secondary dormant and to survive in this stage for many years in the soil. Genetic reduction of secondary seed dormancy in oilseed rape could provide a means to reduce the frequency of volunteer plants and especially the dispersal of transgenic oilseed rape. The objective of the present study was to analyse the inheritance of primary and secondary seed dormancy in a winter oilseed rape doubled haploid population derived from the cross Express 617 × R53 and to study correlations to other seed traits. Field experiments were performed in Germany for 2 years at two locations with two replicates. Seeds harvested from open pollinated plants were used for all analyses, including a laboratory test for seed dormancy. A previously developed molecular marker map of the doubled haploid population was used to map QTL of the relevant traits. For primary, secondary and total seed dormancy, the results showed significant effects of the genotypes and their interactions, with years and locations. Two, four and five QTL were detected for primary, secondary and total seed dormancy which explained 19, 35 and 42 % of the phenotypic variance, respectively. Results show that secondary seed dormancy is a heritable trait and that selection for low secondary seed dormancy is possible.

  2. Ethanol production from rape straw: Part of an oilseed rape biorefinery

    Energy Technology Data Exchange (ETDEWEB)

    Arvaniti, E.

    2010-12-15

    Agricultural residues from rapeseed biodiesel industry (rapeseed cake, rape straw, crude glycerol), which represent the 82%wt. of the oilseed rape, currently have only low-grade applications in the market. For this, a scenario was built on exploiting qualities of rapeseed biodiesel residues for forming added-value products, and expanding and upgrading an existing biodiesel plant, to an oilseed rape biorefinery by 2020 in European ground. Selection of products was based on a technological feasibility study given the time frame, while priority was given to Low-Value-High-Volume readily marketed products, like production of energy and feed. Products selected except rapeseed biodiesel, were ethanol, biogas, enzymes energy, chemical building blocks, and superior quality animal fodder. The production lines were analyzed and prospects for 2020 were projected on a critical basis. Particular merit was given to two products, ethanol from cellulose, and cellulolytic enzymes from rape straw. Cellulosic ethanol from rape straw was optimized for all production steps, i.e. for thermo-chemical pretreatment, enzyme hydrolysis, and fermentation of C6 sugars. Thermo-chemical pretreatment was studied with Wet oxidation technique at different conditions of temperature, reaction time, and oxygen pressure, but also factors like pre-soaking straw in warm water, or recycling liquid were also studied. Wet oxidation has been extensively tested in the past for different substrates, and gives promising results with indicators that are important for cellulosic ethanol production; C6 sugars recovery, high digestibility for enzymes, and limited formed degradation products. Here, optimal pretreatment conditions for rape straw were first presoaking rape straw at 80 deg. C for 20 minutes, and then wet-oxidize with 12 bar of oxygen at 205 deg. C for 3 minutes. Recovery of cellulose and hemicellulose under these conditions was 105% and 106% respectively, while recovery of lignin was 86%. When this

  3. Clubroot – An Emerging Disease Faced by Czech Oilseed Rape Growers

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    Řičařová V.

    2016-09-01

    Full Text Available Clubroot is caused by the pathogen Plasmodiophora brassicae Woronin and has become a serious problem in oilseed rape production in the Czech Republic. The disease was previously widespread in commercial vegetable production and in hobby gardens. Whereas previously restricted only to the NE, since 2010 oilseed rape clubroot has been spreading across the whole country. A five-year monitoring of clubroot occurrence based on disease symptoms detection on oilseed rape fields was accomplished by the Union of Oilseed Growers and Processors. The presence of P. brassicae and clubroot symptoms was reported in all regions of the Czech Republic, except the Ústecký region, and in 31 out of 76 districts. At present, at least 130 fields are known to be infested by the pathogen, but this number is very likely underestimated. Some soil samples were also tested using the conventional polymerase chain reaction (PCR method to evaluate the soil usability. All 14 suspected samples tested positive.

  4. Wild pollinators enhance oilseed rape yield in small-holder farming systems in China.

    Science.gov (United States)

    Zou, Yi; Xiao, Haijun; Bianchi, Felix J J A; Jauker, Frank; Luo, Shudong; van der Werf, Wopke

    2017-02-21

    Insect pollinators play an important role in crop pollination, but the relative contribution of wild pollinators and honey bees to pollination is currently under debate. There is virtually no information available on the strength of pollination services and the identity of pollination service providers from Asian smallholder farming systems, where fields are small, and variation among fields is high. We established 18 winter oilseed rape (Brassica napus L.) fields along a large geographical gradient in Jiangxi province in China. In each field, oilseed rape plants were grown in closed cages that excluded pollinators and open cages that allowed pollinator access. The pollinator community was sampled by pan traps for the entire oilseed rape blooming period. Oilseed rape plants from which insect pollinators were excluded had on average 38% lower seed set, 17% lower fruit set and 12% lower yield per plant, but the seeds were 17% heavier, and the caged plants had 28% more flowers and 18% higher aboveground vegetative biomass than plants with pollinator access. Oilseed rape plants thus compensate for pollination deficit by producing heavier seeds and more flowers. Regression analysis indicated that local abundance and diversity of wild pollinators were positively associated with seed set and yield/straw ratio, while honey bee abundance was not related to yield parameters. Wild pollinator abundance and diversity contribute to oilseed rape yield by enhancing plant resource allocation to seeds rather than to above-ground biomass. This study highlights the importance of the conservation of wild pollinators to support oilseed rape production in small-holder farming systems in China.

  5. Components of a rice-oilseed rape production system augmented with trichoderma sp. Tri-1 control sclerotinia sclerotiorum on oilseed rape

    Science.gov (United States)

    Sclerotinia sclerotiorum causes serious yield losses on many crops throughout the world. In two field trials conducted at the same location in consecutive years, a treatment containing formulated Trichoderma harzianum-1 (Tri-1) resulted in oilseed rape seed yield that was significantly greater than...

  6. Low level impurities in imported wheat are a likely source of feral transgenic oilseed rape (Brassica napus L.) in Switzerland.

    Science.gov (United States)

    Schulze, Juerg; Brodmann, Peter; Oehen, Bernadette; Bagutti, Claudia

    2015-11-01

    In Switzerland, the cultivation of genetically modified (GM) oilseed rape (Brassica napus L.) and the use of its seeds for food and feed are not permitted. Nevertheless, the GM oilseed rape events GT73, MS8×RF3, MS8 and RF3 have recently been found in the Rhine port of Basel, Switzerland. The sources of GM oilseed rape seeds have been unknown. The main agricultural good being imported at the Rhine port of Basel is wheat and from 2010 to 2013, 19% of all Swiss wheat imports originated from Canada. As over 90% of all oilseed rape grown in Canada is GM, we hypothesised that imports of Canadian wheat may contain low level impurities of GM oilseed rape. Therefore, waste fraction samples gathered during the mechanical cleaning of Canadian wheat from two Swiss grain mills were analysed by separating oilseed rape seeds from waste fraction samples and testing DNA of pooled seeds for the presence of transgenes by real-time PCR. Furthermore, oilseed rape seeds from each grain mill were sown in a germination experiment, and seedling DNA was tested for the presence of transgenes by real-time PCR. GT73, MS8×RF3, MS8 and RF3 oilseed rape was detected among seed samples and seedlings of both grain mills. Based on this data, we projected a mean proportion of 0.005% of oilseed rape in wheat imported from Canada. Besides Canadian wheat, the Rhine port of Basel does not import any other significant amounts of agricultural products from GM oilseed rape producing countries. We therefore conclude that Canadian wheat is the major source of unintended introduction of GM oilseed rape seeds into Switzerland.

  7. Evaluation of the Minolta SPAD-502 meter for nitrogen management of oilseed rape

    Science.gov (United States)

    Zhu, Zhe-yan; He, Yong; Huang, Min

    2006-09-01

    Nitrogen is important for oilseed rape production and even moderate deficiencies will substantially reduce yield and profit, but excessive N can pollute both surface and ground water. Thus, farmers are walking a thin line. In order to find the relationship between the SPAD value and the nitrogen content of oilseed rapes, a Minolta SPAD-502 Meter can be used for on-farm measurement of the N content, and then the Kjeldahl method has been used for measuring the nitrogen content of these oilseed rapes. Every leaf had 10 points SPAD value in average for measuring by SPAD, according to the area of the leaves, the points were increased or decreased. The Kjeldahl method has been used for organic nitrogen determination for over a century. Before using the Kjeldahl method to measuring the nitrogen content, all the leaves has been washed and dried. The results of these two methods has been fitted, as a result, the correlation coefficient is 0.863. Another sample was used to check if the result was perfect, the error of the forecasting value is about 7.4%. A new method for measuring the nitrogen content quickly of oilseed rapes has been found, the nitrogen content of the oilseed rape can be measured nondestructively and quickly.

  8. Cadmium uptake and speciation changes in the rhizosphere of cadmium accumulator and non-accumulator oilseed rape varieties.

    Science.gov (United States)

    Su, Dechun; Xing, Jianping; Jiao, Weiping; Wong, Woonchung

    2009-01-01

    Characteristics of cadmium (Cd) uptake kinetics and distribution of Cd speciation in the rhizosphere for Cd accumulator and non-accumulator oilseed rape varieties were investigated under nutrient solution and rhizobox soil culture conditions. The results showed that the maximal influx (V(max)) for Cd2+ and Km were significantly different for the two oilseed rape varieties. The value of V(max) for Cd accumulator oilseed rape Zhucang Huazi was two-fold greater than that for oilseed rape Chuan you II-93. The exchangeable Cd concentration in the rhizosphere was significantly lower than in non-rhizospheric soils supplemented with CdSO4 for both the varieties. Carbonate-bound Cd in the rhizosphere of Cd accumulator oilseed rape was significantly higher than that in the rhizosphere of non-accumulator oilseed rape and non-rhizospheric soil. Cd accumulator oilseed rape had a higher Cd2+ affinity and more ability to uptake insoluble Cd in the soil than the non-accumulator oilseed rape.

  9. Remote Estimation of Vegetation Fraction and Flower Fraction in Oilseed Rape with Unmanned Aerial Vehicle Data

    Directory of Open Access Journals (Sweden)

    Shenghui Fang

    2016-05-01

    Full Text Available This study developed an approach for remote estimation of Vegetation Fraction (VF and Flower Fraction (FF in oilseed rape, which is a crop species with conspicuous flowers during reproduction. Canopy reflectance in green, red, red edge and NIR bands was obtained by a camera system mounted on an unmanned aerial vehicle (UAV when oilseed rape was in the vegetative growth and flowering stage. The relationship of several widely-used Vegetation Indices (VI vs. VF was tested and found to be different in different phenology stages. At the same VF when oilseed rape was flowering, canopy reflectance increased in all bands, and the tested VI decreased. Therefore, two algorithms to estimate VF were calibrated respectively, one for samples during vegetative growth and the other for samples during flowering stage. The results showed that the Visible Atmospherically Resistant Index (VARIgreen worked most accurately for estimating VF in flower-free samples with an Root Mean Square Error (RMSE of 3.56%, while the Enhanced Vegetation Index (EVI2 was the best in flower-containing samples with an RMSE of 5.65%. Based on reflectance in green and NIR bands, a technique was developed to identify whether a sample contained flowers and then to choose automatically the appropriate algorithm for its VF estimation. During the flowering season, we also explored the potential of using canopy reflectance or VIs to estimate FF in oilseed rape. No significant correlation was observed between VI and FF when soil was visible in the sensor’s field of view. Reflectance at 550 nm worked well for FF estimation with coefficient of determination (R2 above 0.6. Our model was validated in oilseed rape planted under different nitrogen fertilization applications and in different phenology stages. The results showed that it was able to predict VF and FF accurately in oilseed rape with RMSE below 6%.

  10. Inheritance of oilseed rape (Brassica napus) RAPD markers in a backcross progeny with Brassica campestris

    DEFF Research Database (Denmark)

    Mikkelsen, T.R.; Jensen, J.; Bagger Jørgensen, Rikke

    1996-01-01

    Different cultivars/transgenic lines of oilseed rape (Brassica napus) were crossed (as females) with different cultivars/populations of Brassica campestris. All cross combinations produced seed, with an average seed set per pollination of 9.8. Backcrossing of selected interspecific hybrids (as...... markers could be assigned to six linkage groups, most probably reflecting six B. napus C-chromosomes. The presence of backcross plants with recombinant genotypes suggests that complex genetic processes can take place during the interspecific hybridisation and backcrossing in these Brassica species....... The implications of our results for the possible choice nf integration sites of transgenes in oilseed rape are discussed....

  11. Solubilization and characterization of diacylglycerol acyltransferase from microspore-derived cultures of oilseed rape.

    OpenAIRE

    Little, D.; Weselake, R; Pomeroy, K; Furukawa-Stoffer, T; Bagu, J

    1994-01-01

    Particulate fractions prepared from microspore-derived (MD) embryos of oilseed rape (Brassica napus L. cv. Reston) and an embryogenic MD cell suspension culture of oilseed rape (B. napus L. cv. Jet Neuf) were used as a source of diacylglycerol acyltransferase (DGAT, EC 2.3.1.20) for enzyme characterization and development of a solubilization procedure. DGAT activity in the 1500-100,000 g fraction from MD embryos was stimulated 4-5-fold by 3 to 4 mg of BSA/ml of reaction mixture. DGAT activity...

  12. Quantitative Multilevel Analysis of Central Metabolism in Developing Oilseeds of Oilseed Rape during in Vitro Culture.

    Science.gov (United States)

    Schwender, Jörg; Hebbelmann, Inga; Heinzel, Nicolas; Hildebrandt, Tatjana; Rogers, Alistair; Naik, Dhiraj; Klapperstück, Matthias; Braun, Hans-Peter; Schreiber, Falk; Denolf, Peter; Borisjuk, Ljudmilla; Rolletschek, Hardy

    2015-07-01

    Seeds provide the basis for many food, feed, and fuel products. Continued increases in seed yield, composition, and quality require an improved understanding of how the developing seed converts carbon and nitrogen supplies into storage. Current knowledge of this process is often based on the premise that transcriptional regulation directly translates via enzyme concentration into flux. In an attempt to highlight metabolic control, we explore genotypic differences in carbon partitioning for in vitro cultured developing embryos of oilseed rape (Brassica napus). We determined biomass composition as well as 79 net fluxes, the levels of 77 metabolites, and 26 enzyme activities with specific focus on central metabolism in nine selected germplasm accessions. Overall, we observed a tradeoff between the biomass component fractions of lipid and starch. With increasing lipid content over the spectrum of genotypes, plastidic fatty acid synthesis and glycolytic flux increased concomitantly, while glycolytic intermediates decreased. The lipid/starch tradeoff was not reflected at the proteome level, pointing to the significance of (posttranslational) metabolic control. Enzyme activity/flux and metabolite/flux correlations suggest that plastidic pyruvate kinase exerts flux control and that the lipid/starch tradeoff is most likely mediated by allosteric feedback regulation of phosphofructokinase and ADP-glucose pyrophosphorylase. Quantitative data were also used to calculate in vivo mass action ratios, reaction equilibria, and metabolite turnover times. Compounds like cyclic 3',5'-AMP and sucrose-6-phosphate were identified to potentially be involved in so far unknown mechanisms of metabolic control. This study provides a rich source of quantitative data for those studying central metabolism. © 2015 American Society of Plant Biologists. All Rights Reserved.

  13. Quantitative Multilevel Analysis of Central Metabolism in Developing Oilseeds of Oilseed Rape During In Vitro Culture

    Energy Technology Data Exchange (ETDEWEB)

    Schwender, Jorg [Brookhaven National Lab. (BNL), Upton, NY (United States); Hebbelmann, Inga [Brookhaven National Lab. (BNL), Upton, NY (United States); Heinzel, Nicholas [Leibniz Inst. of Plant Genetics and Crop Plant Research, Gatersleben (Germany); Hildebrandt, Tatjana [Univ. of Hannover (Germany); Rogers, Alistair [Brookhaven National Lab. (BNL), Upton, NY (United States); Naik, Dhiraj [Brookhaven National Lab. (BNL), Upton, NY (United States); Indian Inst. of Advanced Research Koba, Gujarat (India); Klapperstuck, Matthias [Monash Univ., Melbourne, VIC (Australia); Braun, Hans -Peter [Univ. of Hannover (Germany); Schreiber, Falk [Monash Univ., Melbourne, VIC (Australia); Univ. Halle-Wittenberg, Melbourne (Australia); Denolf, Peter [Bayer CropScience (Belgium); Borisjuk, Ljudmilla [Leibniz Inst. of Plant Genetics and Crop Plant Research, Gatersleben (Germany); Rolletschek, Hardy [Leibniz Inst. of Plant Genetics and Crop Plant Research, Gatersleben (Germany)

    2015-07-01

    Seeds provide the basis for many food, feed, and fuel products. Continued increases in seed yield, composition, and quality require an improved understanding of how the developing seed converts carbon and nitrogen supplies into storage. Current knowledge of this process is often based on the premise that transcriptional regulation directly translates via enzyme concentration into flux. In an attempt to highlight metabolic control, we explore genotypic differences in carbon partitioning for in vitro cultured developing embryos of oilseed rape (Brassica napus). We determined biomass composition as well as 79 net fluxes, the levels of 77 metabolites, and 26 enzyme activities with specific focus on central metabolism in nine selected germplasm accessions. We observed a tradeoff between the biomass component fractions of lipid and starch. With increasing lipid content over the spectrum of genotypes, plastidic fatty acid synthesis and glycolytic flux increased concomitantly, while glycolytic intermediates decreased. The lipid/starch tradeoff was not reflected at the proteome level, pointing to the significance of (posttranslational) metabolic control. Enzyme activity/flux and metabolite/flux correlations suggest that plastidic pyruvate kinase exerts flux control and that the lipid/starch tradeoff is most likely mediated by allosteric feedback regulation of phosphofructokinase and ADP-glucose pyrophosphorylase. Also, quantitative data were used to calculate in vivo mass action ratios, reaction equilibria, and metabolite turnover times. Compounds like cyclic 3',5'-AMP and sucrose-6-phosphate were identified to potentially be involved in so far unknown mechanisms of metabolic control. This study provides a rich source of quantitative data for those studying central metabolism..

  14. Weevils of the genus Ceutorhynchus Germ associated with oilseed rape in northern Serbia

    Directory of Open Access Journals (Sweden)

    Ivan Sivčev

    2015-09-01

    Full Text Available Our research of the genus Ceutorhynchus associated with oilseed rape was conducted in northern parts of Serbia (Stari Žednik, Subotica. A specific assemblage of eight weevil species was found. The most numerous were stem weevils Ceutorhynchus napi with 81% of all trapped specimens, followed by C. pallidactylus with 18%. The remaining six weevil specimens were C. obstrictus, C.erysimi, C. minutus, C. picitarsis, C. sulcicollis and C. typhae, comprising 1% of all trapped insects. Stem weevils C. napi and C. pallidactylus began to immigrate into the oilseed rape fields in autumn, as early as November 10 (BBCH 17-18. The maximum flight was recorded at the beginning of oilseed rape stem elongation (BBCH 22-25 on March 23. A new generation of C. pallidactylus emerged from oilseed rape fields in June (BBCH 76-88, while individual specimens occurred during the autumn. The majority of C. napi emerged in March next year, sporadically until May 7. As a consequence of C. napi dominance insecticide treatment should be performed earlier than usual, i.e. when C. pallidactilus is the primary target.

  15. Early osmotic adjustment responses in drought-resistant and drought-sensitive oilseed rape.

    Science.gov (United States)

    Hatzig, Sarah; Zaharia, L Irina; Abrams, Suzanne; Hohmann, Marie; Legoahec, Laurie; Bouchereau, Alain; Nesi, Nathalie; Snowdon, Rod J

    2014-08-01

    The impact of osmotic stress on growth, physiology, and metabolism of winter oilseed rape (Brassica napus L.) was investigated by detailed analysis of biomass traits, hormone metabolites and osmolytes in two genetically unrelated drought-tolerant genotypes and two unrelated drought-sensitive genotypes. Seedlings were grown in vitro under controlled conditions and osmotic stress was simulated by applying a gradual treatment with polyethylene glycol (PEG 6000), followed by hypo-osmotic treatment of variants used for metabolite determination. The results provide a basis for the identification of reliable selection criteria for drought resistance in oilseed rape. The in vitro cultivation system established during this study enabled effective discrimination of early osmotic stress responses between drought-resistant and -susceptible oilseed rape genotypes that also show large differences in relative seed yield under drought conditions in the field. Clear physiological and metabolic differences were observed between the drought-resistant and drought-sensitive genotypes, suggesting that osmotic adjustment is a key component of drought response in oilseed rape. Unexpectedly, however, the drought-resistant genotypes did not show typical hormonal adjustment and osmolyte accumulation, suggesting that they possess alternative physiological mechanisms enabling avoidance of stress symptoms. © 2014 Institute of Botany, Chinese Academy of Sciences.

  16. Potential bioetanol and biogas production using lignocellulosic biomass from winter rye, oilseed rape and faba bean

    DEFF Research Database (Denmark)

    Petersson, Anneli; Thomsen, Mette Hedegaard; Hauggaard-Nielsen, Henrik

    2007-01-01

    To meet the increasing need for bioenergy several raw materials have to be considered for the production of e.g. bioethanol and biogas. In this study, three lignocellulosic raw materials were studied, i.e. (1) winter rye straw (Secale cereale L), (2) oilseed rape straw (Brassica napus L.) and (3)...

  17. Assessing winter oilseed rape freeze injury based on Chinese HJ remote sensing data*

    Science.gov (United States)

    She, Bao; Huang, Jing-feng; Guo, Rui-fang; Wang, Hong-bin; Wang, Jing

    2015-01-01

    The winter oilseed rape (Brassica napus L.) accounts for about 90% of the total acreage of oilseed rape in China. However, it suffers the risk of freeze injury during the winter. In this study, we used Chinese HJ-1A/1B CCD sensors, which have a revisit frequency of 2 d as well as 30 m spatial resolution, to monitor the freeze injury of oilseed rape. Mahalanobis distance-derived growing regions in a normal year were taken as the benchmark, and a mask method was applied to obtain the growing regions in the 2010–2011 growing season. The normalized difference vegetation index (NDVI) was chosen as the indicator of the degree of damage. The amount of crop damage was determined from the difference in the NDVI before and after the freeze. There was spatial variability in the amount of crop damage, so we examined three factors that may affect the degree of freeze injury: terrain, soil moisture, and crop growth before the freeze. The results showed that all these factors were significantly correlated with freeze injury degree (P<0.01, two-tailed). The damage was generally more serious in low-lying and drought-prone areas; in addition, oilseed rape planted on south- and west-oriented facing slopes and those with luxuriant growth status tended to be more susceptible to freeze injury. Furthermore, land surface temperature (LST) of the coldest day, soil moisture, pre-freeze growth and altitude were in descending order of importance in determining the degree of damage. The findings proposed in this paper would be helpful in understanding the occurrence and severity distribution of oilseed rape freeze injury under certain natural or vegetation conditions, and thus help in mitigation of this kind of meteorological disaster in southern China. PMID:25644468

  18. Assessing winter oilseed rape freeze injury based on Chinese HJ remote sensing data.

    Science.gov (United States)

    She, Bao; Huang, Jing-feng; Guo, Rui-fang; Wang, Hong-bin; Wang, Jing

    2015-02-01

    The winter oilseed rape (Brassica napus L.) accounts for about 90% of the total acreage of oilseed rape in China. However, it suffers the risk of freeze injury during the winter. In this study, we used Chinese HJ-1A/1B CCD sensors, which have a revisit frequency of 2 d as well as 30 m spatial resolution, to monitor the freeze injury of oilseed rape. Mahalanobis distance-derived growing regions in a normal year were taken as the benchmark, and a mask method was applied to obtain the growing regions in the 2010-2011 growing season. The normalized difference vegetation index (NDVI) was chosen as the indicator of the degree of damage. The amount of crop damage was determined from the difference in the NDVI before and after the freeze. There was spatial variability in the amount of crop damage, so we examined three factors that may affect the degree of freeze injury: terrain, soil moisture, and crop growth before the freeze. The results showed that all these factors were significantly correlated with freeze injury degree (P<0.01, two-tailed). The damage was generally more serious in low-lying and drought-prone areas; in addition, oilseed rape planted on south- and west-oriented facing slopes and those with luxuriant growth status tended to be more susceptible to freeze injury. Furthermore, land surface temperature (LST) of the coldest day, soil moisture, pre-freeze growth and altitude were in descending order of importance in determining the degree of damage. The findings proposed in this paper would be helpful in understanding the occurrence and severity distribution of oilseed rape freeze injury under certain natural or vegetation conditions, and thus help in mitigation of this kind of meteorological disaster in southern China.

  19. Dispersal of solitary bees and bumblebees in a winter oilseed rape field

    DEFF Research Database (Denmark)

    Calabuig, Isabel

    2000-01-01

    Dispersal distributions of solitary bees and bumblebees were studied in a winter oilseed rape field. Window-traps were placed in the rape field along a line transect perpendicular to the field edge. 19 species of solitary bees were recorded and all but four species are polylectic, including...... Brassicaceae as host-plant family. Through non-linear regression, the decline in solitary bee individuals versus distance from field edge significantly fitted a steep two-parameter exponential decay function. Activity of solitary bees was clearly highest within 30 metres from the field edge. Apparently......, solitary bees do not play any noteworthy role in the pollination of winter oilseed rape in Denmark. The traps yielded ten species of bumblebees, and a significant linear correlation was found between numbers of individuals and distance from the field edge. This result is attributed to bumblebee foraging...

  20. Unexpected diversity of feral genetically modified oilseed rape (Brassica napus L.) despite a cultivation and import ban in Switzerland

    National Research Council Canada - National Science Library

    Schulze, Juerg; Frauenknecht, Tina; Brodmann, Peter; Bagutti, Claudia

    2014-01-01

    Despite cultivation and seed import bans of genetically modified (GM) oilseed rape (Brassica napus L.), feral GM plants were found growing along railway lines and in port areas at four sites in Switzerland in 2011 and 2012...

  1. Significant reductions in oil quality and lipid content of oilseed rape (Brassica napus L.) under climate change

    DEFF Research Database (Denmark)

    Namazkar, Shahla; Egsgaard, Helge; Frenck, Georg

    Despite of the potential importance to food and bioenergy purposes effects from climate change on plant oil quality have hardly been characterized. Worldwide Brassica napus, rapeseed or oilseed rape, is the second largest source of vegetable oil and the predominant oil crop in Europe. We found...... significant changes in oil quality and quantity of cultivars of oilseed rape grown in five future climate scenarios with elevated [CO2], [O3], temperature and combinations hereof (~RCP8.5, IPCC 2013)....

  2. Unexpected diversity of feral genetically modified oilseed rape (Brassica napus L. despite a cultivation and import ban in Switzerland.

    Directory of Open Access Journals (Sweden)

    Juerg Schulze

    Full Text Available Despite cultivation and seed import bans of genetically modified (GM oilseed rape (Brassica napus L., feral GM plants were found growing along railway lines and in port areas at four sites in Switzerland in 2011 and 2012. All GM plants were identified as glyphosate-resistant GM event GT73 (Roundup Ready, Monsanto. The most affected sites were the Rhine port of Basel and the St. Johann freight railway station in Basel. To assess the distribution and intra- and interspecific outcrossing of GM oilseed rape in more detail, we monitored these two sites in 2013. Leaves and seed pods of feral oilseed rape plants, their possible hybridization partners and putative hybrid plants were sampled in monthly intervals and analysed for the presence of transgenes by real-time PCR. Using flow cytometry, we measured DNA contents of cell nuclei to confirm putative hybrids. In total, 2787 plants were sampled. The presence of GT73 oilseed rape could be confirmed at all previously documented sampling locations and was additionally detected at one new sampling location within the Rhine port. Furthermore, we found the glufosinate-resistant GM events MS8xRF3, MS8 and RF3 (all traded as InVigor, Bayer at five sampling locations in the Rhine port. To our knowledge, this is the first time that feral MS8xRF3, MS8 or RF3 plants were detected in Europe. Real-time PCR analyses of seeds showed outcrossing of GT73 into two non-GM oilseed rape plants, but no outcrossing of transgenes into related wild species was observed. We found no hybrids between oilseed rape and related species. GM plants most frequently occurred at unloading sites for ships, indicating that ship cargo traffic is the main entry pathway for GM oilseed rape. In the future, it will be of major interest to determine the source of GM oilseed rape seeds.

  3. Meteorological and landscape influences on pollen beetle immigration into oilseed rape crops.

    Science.gov (United States)

    Skellern, Matthew P; Welham, Sue J; Watts, Nigel P; Cook, Samantha M

    2017-04-01

    Heavy reliance on pesticide inputs to maintain crop yields has been an important aspect of agricultural intensification. Insecticide use has had detrimental impacts on pollinators and natural pest control agents, contributing to a decline in associated ecosystem services, and has also led to resistance development in pest populations. Throughout Europe, in oilseed rape (Brassica napus L.) crops, prophylactic use of insecticides against pollen beetles (Meligethes aeneus F. also known as Brassicogethes aeneus) has led to such issues, and there is an urgent need to develop more sustainable pest management practices for the crop. Although advice is available to oilseed rape growers regarding control thresholds, it may not be adhered to due to the expense of pollen beetle monitoring relative to the inexpensive cost of pyrethroid insecticides. Thus, the key to reducing prophylactic insecticide applications may lie with improved, less labour intensive methods of pollen beetle monitoring. For these to be realized, a better understanding is needed of the effects of agri-landscape features and meteorological conditions on pollen beetle immigration into the crop. In this study, based on data from four years of pollen beetle monitoring on a total of 41 field sites, we model the effects of meteorological (wind speed and direction, rainfall and accumulated temperature) and landscape (areas of woodland, residential gardens, the current and previous seasons' oilseed rape crops, and lengths of hedgerows and treelines) variables on directional sticky trap catches, at both the single trap and field scales. Meteorological variables, particularly accumulated temperature and wind speed were more important than landscape variables in predicting the abundance of pollen beetles immigrating into OSR fields. Sticky traps that were facing downwind caught more beetles than those that were facing across-wind or upwind; this is the first study to show at a landscape-scale, direct evidence for

  4. Indications of Selenium Protection against Cadmium and Lead Toxicity in Oilseed Rape (Brassica napus L.)

    Science.gov (United States)

    Wu, Zhilin; Yin, Xuebin; Bañuelos, Gary S.; Lin, Zhi-Qing; Liu, Ying; Li, Miao; Yuan, Linxi

    2016-01-01

    The present study investigated the beneficial role of selenium (Se) in protecting oilseed rape (Brassica napus L.) plants from cadmium (Cd+2) and lead (Pb+2) toxicity. Exogenous Se markedly reduced Cd and Pb concentration in both roots and shoots. Supplementation of the medium with Se (5, 10, and 15 mg kg-1) alleviated the negative effect of Cd and Pb on growth and led to a decrease in oxidative damages caused by Cd and Pb. Furthermore, Se-enhanced superoxide free radicals (O2•¯), hydrogen peroxide (H2O2), and lipid peroxidation, as indicated by malondialdehyde accumulation, but decreased superoxide dismutase and glutathione peroxidase activities. Meanwhile, the presence of Cd and Pb in the medium affected Se speciation in shoots. The results suggest that Se could alleviate Cd and Pb toxicity by preventing oxidative stress in oilseed rape plant. PMID:28018407

  5. Effects of Field Conditions on Emergence of Oilseed Rape Seed lots Grown in Khorasan Province

    Directory of Open Access Journals (Sweden)

    A Yanegh

    2014-07-01

    Full Text Available Nineteen irrigated farms of canola from six cities of Khorasan selected to assess the seedling emergence one month after sowing in 2009. Soil texture of each farm determined using the Hydrometer method. The results showed that weather conditions of sowing areas had no significant effect on emergence of oilseed rape. With increasing the temperature (within the range of 19.5 to 21.5 ˚C emergence percentage was not increased significantly. Rainfall also caused no significant effect on seedling emergence. The percentage of emergence in machine-sowing and hand-sowing were 38.4% and 28%, respectively. Soils with loam silt, silt clay loam and loam texture had emergence of 27.2%, 31.2% and 47% respectively. It seems soil texture plays a great roll on the emergence of oilseed rape with epigeal seedlings.

  6. Indications of selenium protection against cadmium and lead toxicity in oilseed rape (Brassica napus L.

    Directory of Open Access Journals (Sweden)

    Zhilin Wu

    2016-12-01

    Full Text Available The present study investigated the beneficial role of selenium (Se in protecting oilseed rape (Brassica napus L. plants from cadmium (Cd+2 and lead (Pb+2 toxicity. Exogenous Se markedly reduced Cd and Pb concentration in both roots and shoots. Supplementation of the medium with Se (5, 10 and 15 mg kg-1 alleviated the negative effect of Cd and Pb on growth and led to a decrease in oxidative damages caused by Cd and Pb. Furthermore, Se enhanced superoxide free radicals (O2-, hydrogen peroxide (H2O2 and lipid peroxidation, as indicated by malondialdehyde (MDA accumulation, but decreased superoxide dismutase (SOD and glutathione peroxidase (GPx activities. Meanwhile, the presence of Cd and Pb in the medium affected Se speciation in shoots. The results suggest that Se could alleviate Cd and Pb toxicity by preventing oxidative stress in oilseed rape plant.

  7. Ozone dose-response relationships for spring oilseed rape and broccoli

    Science.gov (United States)

    De Bock, Maarten; Op de Beeck, Maarten; De Temmerman, Ludwig; Guisez, Yves; Ceulemans, Reinhart; Vandermeiren, Karine

    2011-03-01

    Tropospheric ozone is an important air pollutant with known detrimental effects for several crops. Ozone effects on seed yield, oil percentage, oil yield and 1000 seed weight were examined for spring oilseed rape ( Brassica napus cv. Ability). For broccoli ( Brassica oleracea L. cv. Italica cv. Monaco) the effects on fresh marketable weight and total dry weight were studied. Current ozone levels were compared with an increase of 20 and 40 ppb during 8 h per day, over the entire growing season. Oilseed rape seed yield was negatively correlated with ozone dose indices calculated from emergence until harvest. This resulted in an R2 of 0.24 and 0.26 ( p broccoli the applied ozone doses had no effect on yield.

  8. Industrially processed oilseed rape in the production of table eggs

    Directory of Open Access Journals (Sweden)

    Mária Angelovičová

    2016-07-01

    Full Text Available The purpose of this study was to investigate the effectiveness of feed mixtures with varying proportions of rape cakes to the weight of table eggs, its components, thickness and strength of egg shell. The eggs were from the final laying hybrid ISA Brown reared in the enriched cage system under experimental conditions. An age of laying hens was from 48 to 54 weeks. Egg weight and its components were measured on scales type KERN 440-35N, with an accuracy of 0.01 g and a maximum weight of 400 g. Egg white weight was calculated. The thickness and strength of the egg shell were measured from the dried samples at 55 °C. From each egg shell were cut 3 pcs of samples in the equatorial plane, one sample from the blunt end and one sample from the sharp end. Egg shell thickness was measured by test instrument SOME, type 60/0.01mm with a range of 0 - 10 mm. Egg shell strength was measured according to test instrument Instron with the small body, having a diameter 4.48 mm to exert pressure on the egg shell. The obtained data were assessed in the program system SAS, version 8.2. Based on the results observed in egg weight of our experiment we can conclude that in the group with share 5% of rape cakes was non-statistically significant (p >0.05 decreased egg weight compared to the control group. Egg weight was reduced in the group with share 10% of rape cakes, which confirmed a statistically significant difference compared to egg weight of control group (p ˂0.05. The differences among experimental groups with share 5% and 10% of rape cakes in feed mixture and as well as to control group were not statistically significant (p >0.05 in weight of egg yolk, egg white, egg shell and egg shell strength. Egg shell thickness was no statistically significant (p >0.05 increased in experimental group with share 5% of rape cakes and decreased in experimental group with share 10% of rape cakes versus control group. Increase of egg shell thickness in experimental group with

  9. Distance from forest edge affects bee pollinators in oilseed rape fields

    OpenAIRE

    Bailey, S.; Requier, F.; Nusillard, B.; Roberts, Stuart; Potts, Simon; Bouget, C.

    2014-01-01

    Wild pollinators have been shown to enhance the pollination of Brassica napus(oilseed rape) and thus increase its market value. Several studies have previously shown that pollination services are greater in crops adjoining forest patches or other seminatural habitats than in crops completely surrounded by other crops.\\ud In this study, we investigated the specific importance of forest edges in providing potential pollinators in B. napus fields in two areas in France. Bees were caught with yel...

  10. Potential bioethanol and biogas production using lignocellulosic biomass from winter rye, oilseed rape and faba bean

    Energy Technology Data Exchange (ETDEWEB)

    Petersson, Anneli; Thomsen, Mette H.; Hauggaard-Nielsen, Henrik; Thomsen, Anne-Belinda [Risoe National Laboratory, P.O. Box 49, DK-4000 Roskilde (Denmark)

    2007-11-15

    To meet the increasing need for bioenergy several raw materials have to be considered for the production of e.g. bioethanol and biogas. In this study, three lignocellulosic raw materials were studied, i.e. (1) winter rye straw (Secale cereale L), (2) oilseed rape straw (Brassica napus L.) and (3) faba bean straw (Viciafaba L.). Their composition with regard to cellulose, hemicellulose, lignin, extractives and ash was evaluated, as well as their potential as raw materials for ethanol and biogas production. The materials were pretreated by wet oxidation using parameters previously found to be optimal for pretreatment of corn stover (195 C, 15 min, 2 g l{sup -1} Na{sub 2}CO{sub 3} and 12 bar oxygen). It was shown that pretreatment was necessary for ethanol production from all raw materials and gave increased biogas yield from winter rye straw. Neither biogas productivity nor yield from oilseed rape straw or faba bean straw was significantly affected by pretreatment. Ethanol was produced by the yeast Saccharomyces cerevisiae during simultaneous enzymatic hydrolysis of the solid material after wet oxidation with yields of 66%, 70% and 52% of theoretical for winter rye, oilseed rape and faba bean straw, respectively. Methane was produced with yields of 0.36, 0.42 and 0.44 l g{sup -1} volatile solids for winter rye, oilseed rape and faba bean straw, respectively, without pretreatment of the materials. However, biogas productivity was low and it took over 50 days to reach the final yield. It could be concluded that all three materials are possible raw materials for either biogas or ethanol production; however, improvement of biogas productivity or ethanol yield is necessary before an economical process can be achieved. (author)

  11. Something in the air? The impact of volatiles on mollusc attack of oilseed rape seedlings

    Science.gov (United States)

    Félix, Anne-Emmanuelle; Poppy, Guy M.; Newland, Philip L.; van Dam, Nicole M.; Hanley, Mick E.

    2016-01-01

    Background and Aims Mounting concerns about balancing food security with the environmental impacts of agro-chemical use underpin the need to better understand the mechanisms by which crop plants, particularly during the vulnerable seedling stage, attract or repel herbivores. Methods The feeding preferences of the mollusc Helix aspersa were determined for several oilseed rape (Brassica napus) cultivars and a rank order of acceptability was established. This was compared with glucosinolate concentrations and volatile organic compound (VOC) profiles to determine whether seedling acceptability to molluscs was linked to either form of defence. Key Results While VOC profiles for each oilseed rape cultivar could be separated by canonical discriminant analysis and associated with mollusc feeding preferences, glucosinolate profiles were unrelated to snail feeding behaviour. A mixture of monoterpenes (α-pinene, β-myrcene and δ-3-carene) was identified as a putative attractant, while a blend of the green leaf volatiles 3-hexen-1-ol, 3-hexen-1-ol acetate and the monoterpene α-terpinene was identified as a putative repellent mix. Added to the VOC profile of oilseed rape seedlings, the ‘repellent’ mix reduced mollusc selection, while the ‘attractant’ mix had no effect. Conclusions Despite the widespread assumption that seedling selection by generalist herbivores is governed by chemical defence and taste, we show that olfactory cues may be more important. Oilseed rape may be atypical of wild plants, but our ability to identify repellent volatile organic compounds that can influence snail olfactory selection points to new methods for crop protection using modified VOC profiles during the vulnerable seedling stage. PMID:27009912

  12. Oilseed rape: learning about ancient and recent polyploid evolution from a recent crop species.

    Science.gov (United States)

    Mason, A S; Snowdon, R J

    2016-11-01

    Oilseed rape (Brassica napus) is one of our youngest crop species, arising several times under cultivation in the last few thousand years and completely unknown in the wild. Oilseed rape originated from hybridisation events between progenitor diploid species B. rapa and B. oleracea, both important vegetable species. The diploid progenitors are also ancient polyploids, with remnants of two previous polyploidisation events evident in the triplicated genome structure. This history of polyploid evolution and human agricultural selection makes B. napus an excellent model with which to investigate processes of genomic evolution and selection in polyploid crops. The ease of de novo interspecific hybridisation, responsiveness to tissue culture, and the close relationship of oilseed rape to the model plant Arabidopsis thaliana, coupled with the recent availability of reference genome sequences and suites of molecular cytogenetic and high-throughput genotyping tools, allow detailed dissection of genetic, genomic and phenotypic interactions in this crop. In this review we discuss the past and present uses of B. napus as a model for polyploid speciation and evolution in crop species, along with current and developing analysis tools and resources. We further outline unanswered questions that may now be tractable to investigation. © 2016 German Botanical Society and The Royal Botanical Society of the Netherlands.

  13. Cold plasma treatment enhances oilseed rape seed germination under drought stress

    Science.gov (United States)

    Ling, Li; Jiangang, Li; Minchong, Shen; Chunlei, Zhang; Yuanhua, Dong

    2015-01-01

    Effects of cold plasma treatment on seed germination, seedling growth, antioxidant enzymes, lipid peroxidation levels and osmotic-adjustment products of oilseed rape under drought stress were investigated in a drought-sensitive (Zhongshuang 7) and drought-tolerant cultivar (Zhongshuang 11). Results showed that, under drought stress, cold plasma treatment significantly improved the germination rate by 6.25% in Zhongshuang 7, and 4.44% in Zhongshuang 11. Seedling growth characteristics, including shoot and root dry weights, shoot and root lengths, and lateral root number, significantly increased after cold plasma treatment. The apparent contact angle was reduced by 30.38% in Zhongshuang 7 and 16.91% in Zhongshuang 11. Cold plasma treatment markedly raised superoxide dismutase and catalase activities by 17.71% and 16.52% in Zhongshuang 7, and by 13.00% and 13.21% in Zhongshuang 11. Moreover, cold plasma treatment significantly increased the soluble sugar and protein contents, but reduced the malondialdehyde content in seedlings. Our results suggested that cold plasma treatment improved oilseed rape drought tolerance by improving antioxidant enzyme activities, increasing osmotic-adjustment products, and reducing lipid peroxidation, especially in the drought-sensitive cultivar (Zhongshuang 7). Thus, cold plasma treatment can be used in an ameliorative way to improve germination and protect oilseed rape seedlings against damage caused by drought stress. PMID:26264651

  14. Response of seed tocopherols in oilseed rape to nitrogen fertilizer sources and application rates* #

    Science.gov (United States)

    Hussain, Nazim; Li, Hui; Jiang, Yu-xiao; Jabeen, Zahra; Shamsi, Imran Haider; Ali, Essa; Jiang, Li-xi

    2014-01-01

    Tocopherols (Tocs) are vital scavengers of reactive oxygen species (ROS) and important seed oil quality indicators. Nitrogen (N) is one of the most important fertilizers in promoting biomass and grain yield in crop production. However, the effect of different sources and application rates of N on seed Toc contents in oilseed rape is poorly understood. In this study, pot trials were conducted to evaluate the effect of two sources of N fertilizer (urea and ammonium nitrate). Each source was applied to five oilseed rape genotypes (Zheshuang 72, Jiu-Er-1358, Zheshuang 758, Shiralee, and Pakola) at three different application rates (0.41 g/pot (N1), 0.81 g/pot (N2), and 1.20 g/pot (N3)). Results indicated that urea increased α-, γ-, and total Toc (T-Toc) more than did ammonium nitrate. N3 was proven as the most efficient application rate, which yielded high contents of γ-Toc and T-Toc. Highly significant correlations were observed between Toc isomers, T-Toc, and α-/γ-Toc ratio. These results clearly demonstrate that N sources and application rates significantly affect seed Toc contents in oilseed rape. PMID:24510711

  15. Influence of top dressing on yield and seed quality components of oilseed rape

    Directory of Open Access Journals (Sweden)

    Vujaković Milka

    2010-01-01

    Full Text Available Oilseed rape is an agronomically important species grown for seed that contains 40-48% oil and 18-25% proteins. Oilseed rape has high demands for nitrogen. The research was performed on four varieties of oilseed rape (Banaćanka, Valeska, Slavica and Express developed at Institute of Field and Vegetable Crops in Novi Sad, during vegetative seasons 2007-2008 and 2008-2009. During vegetation, top dressing was applied with different amounts of KAN: control (0 kg N ha-1, 50 kg N ha-1, 100 kg N ha-1, 150 kg N ha-1 and the N quantity determination were performed applying the balancing method (also known as N-min method. Yield, 1000-seed weight, seed germination, oil and protein content were determined after harvest. Yield and seed germination depended on seed production year. 1000-seed weight depended on seed production year and genotype. Oil and protein content in seed depended on production year, genotype and amount of the N-fertilizer applied by top dressing.

  16. Potential environmental impacts associated with large-scale herbicide-tolerant GM oilseed rape crops

    Directory of Open Access Journals (Sweden)

    Fellous Marc

    2004-07-01

    Full Text Available The Biomolecular Engineering Commission considers that the knowledge acquired in the last three years has provided significant information in reply to the points raised in its review dated 16 February 2001. The Commission has studied the potential environmental impacts associated with large-scale herbicidetolerantGMoilseed rape crops, making a distinction between direct and indirect impacts. Direct impacts stem from the intrinsic properties of herbicide-tolerant GM oilseed rape crops whereas indirect impacts result from practices associated with the farming of these crops. The Commission considers that, in the absence of the use of the herbicide in question in and outside of farmed land, there is no direct environmental risk (development of invasive crops per se associated with the presence of a herbicide-tolerance gene in oilseed rape (or related species. Nevertheless, since the interest of these tolerant crops lies in the use of the herbicide in question, indirect effects, to varying extents, have been identified and must be taken into account: the use of the herbicide in question, applied to agricultural fields containing the herbicide-tolerant crop could lead to an increase in oilseed rape volunteer populations in crop rotations; the selective pressure exerted by non-specific herbicides (to which the crops have been rendered tolerant may be very high in cases of continuous and uncontrolled use of these herbicides, and may result in the persistence of rare events such as the reproduction of fertile interspecies hybrids; the change to the range of herbicides used should be conveyed by more effective weed control and, like any change in farming practices, induce indirect effects on the agri-ecosystem, particularly in terms of changes to weeds and the associated animal life. Accordingly, the Biomolecular Engineering Commission recommends a global approach in terms of the large-scale farming of herbicide-tolerant crops that: accounts for the

  17. Development of new restorer lines for CMS ogura system with the use of resynthesized oilseed rape (Brassica napus L.).

    Science.gov (United States)

    Szała, Laurencja; Sosnowska, Katarzyna; Popławska, Wiesława; Liersch, Alina; Olejnik, Anna; Kozłowska, Katarzyna; Bocianowski, Jan; Cegielska-Taras, Teresa

    2016-09-01

    Resynthesized (RS) oilseed rape (Brassica napus L.) is potentially of great interest for hybrid breeding. However, a major problem with the direct use of RS B. napus is the quality of seed oil (high level of erucic acid) and seed meal (high glucosinolate content), which does not comply with double-low quality oilseed rape. Thus, additional developments are needed before RS B. napus can be introduced into breeding practice. In this study, RS oilseed rape was obtained through crosses between B. rapa ssp. chinensis var. chinensis and B. oleracea ssp. acephala var. sabellica. RS plant was then crossed with double-low (00) winter oilseed rape lines containing the Rfo gene for Ogura cytoplasmic male sterility (CMS ogu) system. Populations of doubled haploids (DH) were developed from these F1 hybrids using the microspore in vitro culture method. The seeds of semi-RS DH lines were analyzed for erucic acid and glucosinolate content. Among the populations of semi-RS DHs four 00-quality lines with the Rfo gene were selected. Using 344 AFLP markers to estimate genetic relatedness, we showed that the RS lines and semi-RS lines formed clusters that were clearly distinct from 96 winter oilseed rape parental lines of F1 hybrids.

  18. Meat and bone meal as nitrogen and phosphorus supplier to cereals and oilseed rape

    Directory of Open Access Journals (Sweden)

    Anna Nogalska

    2014-02-01

    Full Text Available The purpose of this study was to evaluate the effect of meat and bone meal (MBM on cultivation of winter triticale, winter oilseed rape, winter wheat and maize. The average annual yields and protein yield achieved in crop rotation were studied. The field trials were carried out in north-eastern Poland in 2006–2010. The factor was dose of MBM: 1.0, 1.5, 2.0 and 2.5 t ha-1 year-1 or 2.0, 3.0, 4.0 and 5.0 t ha-1 every other year. The four-year experiment has proven that MBM is a valuable nitrogen and phosphorus fertilizer in cultivation of cereals and oilseed rape. By amendment of the tested meal into the soil it produced crop yield and protein yield similar to that achieved by mineral fertilization. However, the crude fat yield of rape was significantly higher under the influence of all the MBM doses. The yield-stimulating effect of MBM did not depend on the frequency of its application; therefore it is more convenient to apply it once every two years. Increasing MBM from 1.5 to 2.5 t ha-1 did not significantly increase any of the four crop yields, therefore for soils that had satisfactory nutrients content, 1 or 1.5 t ha-1 MBM is enough and increasing MBM will only increase economic burden for farmers and environmental risks.

  19. Do competitive conditions affect introgression of transgenes from oilseed rape (Brassica napus) to weedy Brassica rapa? AS case study with special reference to transplastomic oilseed rape

    DEFF Research Database (Denmark)

    Johannessen, Marina

    In species where chloroplast inheritance is exclusively or predominantly maternal, pollen-mediated flow of transgenes is reduced if transgenes are inserted in chloroplast DNA instead of nuclear DNA. However, transmission of chloroplast-encoded transgeneswill still occur if transgenic individuals......) significantly. As to the proportion between the species, B. rapa was a stronger competitor than B. napus. Theproportion seemed to be a more powerful factor than the density. In conclusion, hybridisation on B. napus seems to be most likely at current field densities of B. napus and when B. rapa is an abundant...... affected the thousand-kernel weight significantly. It was concluded that further introgression of transgenes from transplastomic oilseed rape to B. rapa is mostlikely at current field densities of B. napus and when B. rapa is an abundant weed....

  20. Use of formulated Trichoderma sp. Tri-1 in combination with reduced rates of chemical pesticide for control of Sclerotinia sclerotiorium on oilseed rape

    Science.gov (United States)

    Sustainable strategies for control of Sclerotinia sclerotiorum on oilseed rape are needed. Here we tested combinations of Trichoderma sp. Tri-1, formulated with oilseed rape seedcake and straw, with reduced application rates of the chemical pesticide Carbendazim for control of this pathogen on oils...

  1. Effects of plant densities on yield, yield components and some morphological characters of two cultivators of oilseed rape (Brassica napus L.)

    DEFF Research Database (Denmark)

    Al-Barzinjy, M.; Stölen, O.; Christiansen, Jørgen Lindskrog

    2003-01-01

    Effects of Plant Densities on Yield, Yield Components and some Morphological Characters of two Cultivators of Oilseed Rape (Brassica napus L.)......Effects of Plant Densities on Yield, Yield Components and some Morphological Characters of two Cultivators of Oilseed Rape (Brassica napus L.)...

  2. Status of feral oilseed rape in Europe: its minor role as a GM impurity and its potential as a reservoir of transgene persistence

    DEFF Research Database (Denmark)

    Squire, Geoffrey R.; Breckling, Broder; Dietz Pfeilstetter, Antje

    2011-01-01

    Feral oilseed rape has become widespread in Europe on waysides and waste ground. Its potential as a source of GM impurity in oilseed rape harvests is quantified, for the first time, by a consistent analysis applied over a wide range of study areas in Europe. The maximum contribution of feral...

  3. Arthropod Pest Control for UK Oilseed Rape - Comparing Insecticide Efficacies, Side Effects and Alternatives.

    Directory of Open Access Journals (Sweden)

    Han Zhang

    Full Text Available Oilseed rape (Brassica napus is an important combinable break crop in the UK, which is largely protected from arthropod pests by insecticidal chemicals. Despite ongoing debate regarding the use of neonicotinoids, the dominant seed treatment ingredients used for this crop, there is little publicly available data comparing the efficacy of insecticides in controlling key arthropod pests or comparing the impacts on non-target species and the wider environment. To provide an insight into these matters, a UK-wide expert survey targeting agronomists and entomologists was conducted from March to June 2015. Based on the opinions of 90 respondents, an average of 20% yield loss caused by the key arthropod pests was expected to have occurred in the absence of insecticide treatments. Relatively older chemical groups were perceived to have lower efficacy for target pests than newer ones, partly due to the development of insecticide resistance. Without neonicotinoid seed treatments, a lack of good control for cabbage stem flea beetle was perceived. Wide spectrum foliar insecticide sprays were perceived to have significantly greater negative impacts than seed treatments on users' health, natural enemies, pollinators, soil and water, and many foliar active ingredients have had potential risks for non-target arthropod species in UK oilseed rape fields for the past 25 years. Overall, 72% of respondents opposed the neonicotinoid restriction, while 10% supported it. Opposition and support of the restriction were largely based on concerns for pollinators and the wider environment, highlighting the uncertainty over the side effects of neonicotinoid use. More people from the government and research institutes leaned towards neutrality over the issue, compared to those directly involved in growing the crop. Neonicotinoid restriction was expected to result in greater effort and expenditure on pest control and lower production (0-1 t/ha less. Alternatives for future

  4. Ozone effects on yield quality of spring oilseed rape and broccoli

    Science.gov (United States)

    Vandermeiren, Karine; De Bock, Maarten; Horemans, Nele; Guisez, Yves; Ceulemans, Reinhart; De Temmerman, Ludwig

    2012-02-01

    The impact of elevated tropospheric ozone (O 3) on the quality of spring oilseed rape ( Brassica napus cv Ability) and broccoli ( Brassica oleracea L. cv Italic cv Monaco) was assessed during a three year Open - Top Chamber (OTC) experiment. Current ambient O 3 levels were compared to an increase of 20 and 40 ppb during 8 h per day over the entire growing season. The qualitative responses were expressed as a function of the accumulated hourly O 3 concentrations over a threshold of 40 ppb (AOT40) and the phytotoxic O 3 dose above a threshold of 6 nmol s -1 m -2 projected leaf area (POD 6). Our results provide clear evidence that O 3 has an influence on the qualitative attributes of the harvested products of these Brassica species. The responses were comparable whether they were expressed as a function of the accumulated O 3 concentrations or of the modelled O 3 uptake. The protein concentration of oilseed rape seeds and broccoli heads was significantly increased in response to O 3. There was also a shift in the fatty acid composition of the vegetable oil derived from seeds of oilseed rape. Oleic acid (18:1) declined significantly ( p broccoli an important shift occurred from indolic to aliphatic GSLs although the total GSL concentration was not changed. The increase in the aliphatic/indolic GSL ratio ( p broccoli were not influenced by O 3; glutathione (GSH) was slightly increased in response to a higher O 3 uptake ( p < 0.05). The consequences of these changes with regard to food and feed quality and human health are discussed.

  5. Plant regeneration from hypocotyl protoplasts of winter oilseed rape (Brassica napus L.

    Directory of Open Access Journals (Sweden)

    Wacław Orczyk

    2014-01-01

    Full Text Available Protoplasts were isolated from hypocotyls of six breeding lines and two cultivars of winter oilseed rape (B. napus L.. Under presented culture conditions almost all of the protoplasts regenerated cell walls. Division frequency depended on the genotype and was from 50% to 64%. Shoot regeneration (also depended on the genotype was induced with the frequency of 3.6% (for cv Bolko on the medium containing IAA (0.1 mg•dm-3, zeatin (0.5 mg•dm-3 and BAP (0.5 mg•dm-3 . All shoots were rooted on MS basal medium supplemented with sucrose 30 g•dm-3.

  6. Seed Vigor and Field Performance of Winter Oilseed Rape (Brassica napus L.) Cultivars

    OpenAIRE

    GHASSEMI-GOLEZANI, Kazem; Javad BAKHSHY; Yaeghoob RAEY; Ayda HOSSAINZADEH-MAHOOTCHY

    2010-01-01

    The effect of seed vigor on field performance of two oilseed rape cultivars (Licord and Okapi) was investigated in 2008 at the Research Farm of the University Tabriz, Iran. The experiment was arranged as factorial based on RCB design with three replications. A sub-sample of seeds of each cultivar was kept as control. The other two sub-samples of each cultivar with about 15% moisture content were artificially deteriorated at 40C for 9 and 12 days (Licord) and 7 and 10 days (Okapi). Cons...

  7. Large-scale monitoring of effects of clothianidin-dressed oilseed rape seeds on pollinating insects in Northern Germany: effects on honey bees (Apis mellifera).

    Science.gov (United States)

    Rolke, Daniel; Fuchs, Stefan; Grünewald, Bernd; Gao, Zhenglei; Blenau, Wolfgang

    2016-11-01

    Possible effects of clothianidin seed-treated oilseed rape on honey bee colonies were investigated in a large-scale monitoring project in Northern Germany, where oilseed rape usually comprises 25-33 % of the arable land. For both reference and test sites, six study locations were selected and eight honey bee hives were placed at each location. At each site, three locations were directly adjacent to oilseed rape fields and three locations were situated 400 m away from the nearest oilseed rape field. Thus, 96 hives were exposed to fully flowering oilseed rape crops. Colony sizes and weights, the amount of honey harvested, and infection with parasites and diseases were monitored between April and September 2014. The percentage of oilseed rape pollen was determined in pollen and honey samples. After oilseed rape flowering, the hives were transferred to an extensive isolated area for post-exposure monitoring. Total numbers of adult bees and brood cells showed seasonal fluctuations, and there were no significant differences between the sites. The honey, which was extracted at the end of the exposure phase, contained 62.0-83.5 % oilseed rape pollen. Varroa destructor infestation was low during most of the course of the study but increased at the end of the study due to flumethrin resistance in the mite populations. In summary, honey bee colonies foraging in clothianidin seed-treated oilseed rape did not show any detrimental symptoms as compared to colonies foraging in clothianidin-free oilseed rape. Development of colony strength, brood success as well as honey yield and pathogen infection were not significantly affected by clothianidin seed-treatment during this study.

  8. Development of a biologically based fertilizer, incorporating Bacillus megaterium A6, for improved phosphorus nutrition of oilseed rape.

    Science.gov (United States)

    Hu, Xiaojia; Roberts, Daniel P; Xie, Lihua; Maul, Jude E; Yu, Changbing; Li, Yinshui; Zhang, Shujie; Liao, Xing

    2013-04-01

    Sustainable methods with diminished impact on the environment need to be developed for the production of oilseed rape in China and other regions of the world. A biological fertilizer consisting of Bacillus megaterium A6 cultured on oilseed rape meal improved oilseed rape seed yield (P rape meal without strain A6 in 1 of 2 experiments, suggesting a role for strain A6 in improving yield. Strain A6 was capable of solubilizing phosphorus from rock phosphate in liquid culture and produced enzymes capable of mineralizing organic phosphorus (acid phosphatase, phytase) in liquid culture and in the biological fertilizer. The biologically based fertilizer, containing strain A6, improved plant phosphorus nutrition in greenhouse pot experiments resulting in significantly greater available phosphorus in natural soil and in significantly greater plant phosphorus content relative to the nontreated control. Seed yield and available phosphorus in natural soil were significantly greater with a synthetic chemical fertilizer treatment, reduced in phosphorus content, than the biological fertilizer treatment, but a treatment containing the biological fertilizer combined with the synthetic fertilizer provided the significantly greatest seed yield, available phosphorus in natural soil, and plant phosphorus content. These results suggest that the biological fertilizer was capable of improving oilseed rape seed yield, at least in part, through the phosphorus-solubilizing activity of B. megaterium A6.

  9. Phenology of cabbage stem flea beetle (Psylliodes chrysocephala L in oilseed rape

    Directory of Open Access Journals (Sweden)

    Sivčev Lazar

    2016-01-01

    Full Text Available The cabbage stem flea beetle (Psylliodeschrysocephala (L. is an important pest of winter oilseed rape in Serbia. Beetles colonize oilseed rape in early October and are active in the field until first frost and wintertime. In autumn, adults can be seen laying eggs in the soil around plants. Larvae of P. chrysocephala developed intensively on leaf petioles in November, reaching their highest numbers at the end of the month. No infested plants were found in a conventional field, while 14.5% of all dissected leaf petioles were infested on an integrated field. On unprotected plants in an organic field, 76.0% of the plants were infested with larvae at the growth-stage BBCH 18-19, with 31.1% infested leaves on average. As a results, the number of plants was reduced by 51%, i.e. from 43.0/m2 recorded in the autumn to 22.0/m2 in the following spring. A new generation of P. chrysocephala beetles emerged from the soil in the first half of June and rapidly escaped the fields from almost dry plants. Our results showed that a part of the population stayed in aestivation and emerged in the following crop during the next season. On the following 5 March, 0.5 beetles/m2 were found in emergence cages in the organic field, while 0.81 beetles/m2 were found in the former trap crop.

  10. Crop Establishment Practices Are a Driver of the Plant Microbiota in Winter Oilseed Rape (Brassica napus)

    Science.gov (United States)

    Rathore, Ridhdhi; Dowling, David N.; Forristal, Patrick D.; Spink, John; Cotter, Paul D.; Bulgarelli, Davide; Germaine, Kieran J.

    2017-01-01

    Gaining a greater understanding of the plant microbiota and its interactions with its host plant heralds a new era of scientific discovery in agriculture. Different agricultural management practices influence soil microbial populations by changing a soil’s physical, chemical and biological properties. However, the impact of these practices on the microbiota associated with economically important crops such as oilseed rape, are still understudied. In this work we investigated the impact of two contrasting crop establishment practices, conventional (plow based) and conservation (strip–tillage) systems, on the microbiota inhabiting different plant microhabitats, namely rhizosphere, root and shoot, of winter oilseed rape under Irish agronomic conditions. Illumina 16S rRNA gene sequence profiling showed that the plant associated microhabitats (root and shoot), are dominated by members of the bacterial phyla Proteobacteria, Actinobacteria and Bacteroidetes. The root and shoot associated bacterial communities displayed markedly distinct profiles as a result of tillage practices. We observed a very limited ‘rhizosphere effect’ in the root zone of WOSR, i.e., there was little or no increase in bacterial community richness and abundance in the WOSR rhizosphere compared to the bulk soil. The two tillage systems investigated did not appear to lead to any major long term differences on the bulk soil or rhizosphere bacterial communities. Our data suggests that the WOSR root and shoot microbiota can be impacted by management practices and is an important mechanism that could allow us to understand how plants respond to different management practices and environments. PMID:28848510

  11. Crop Establishment Practices Are a Driver of the Plant Microbiota in Winter Oilseed Rape (Brassica napus

    Directory of Open Access Journals (Sweden)

    Ridhdhi Rathore

    2017-08-01

    Full Text Available Gaining a greater understanding of the plant microbiota and its interactions with its host plant heralds a new era of scientific discovery in agriculture. Different agricultural management practices influence soil microbial populations by changing a soil’s physical, chemical and biological properties. However, the impact of these practices on the microbiota associated with economically important crops such as oilseed rape, are still understudied. In this work we investigated the impact of two contrasting crop establishment practices, conventional (plow based and conservation (strip–tillage systems, on the microbiota inhabiting different plant microhabitats, namely rhizosphere, root and shoot, of winter oilseed rape under Irish agronomic conditions. Illumina 16S rRNA gene sequence profiling showed that the plant associated microhabitats (root and shoot, are dominated by members of the bacterial phyla Proteobacteria, Actinobacteria and Bacteroidetes. The root and shoot associated bacterial communities displayed markedly distinct profiles as a result of tillage practices. We observed a very limited ‘rhizosphere effect’ in the root zone of WOSR, i.e., there was little or no increase in bacterial community richness and abundance in the WOSR rhizosphere compared to the bulk soil. The two tillage systems investigated did not appear to lead to any major long term differences on the bulk soil or rhizosphere bacterial communities. Our data suggests that the WOSR root and shoot microbiota can be impacted by management practices and is an important mechanism that could allow us to understand how plants respond to different management practices and environments.

  12. The presence of turnip yellows virus in oilseed rape (Brassica napus L. in Serbia

    Directory of Open Access Journals (Sweden)

    Miloševic Dragana

    2016-01-01

    Full Text Available A total of 86 oilseed rape samples from six crops in different localities were collected during 2014 and analyzed for the presence of Turnip yellows virus (TuYV, Cauliflower mosaic virus (CaMV and Turnip mosaic virus (TuMV using commercial double-antibody sandwich (DAS-ELISA kits. TuYV was serologically detected in 60 collected samples (69.77%, and none of the samples tested were positive for CaMV and TuMV. Six selected TuYV isolates were successfully transmitted by Myzus persicae to three test plants, confirming the infectious nature of the disease. In the selected ELISA-positive samples, the presence of TuYV was further confirmed by RT-PCR and sequencing. A comparison of the obtained sequence with those available in GenBank confirmed the presence of TuYV in oilseed rape samples. An analysis of P0 gene sequence data for a subset of these isolates showed they clustered with the known TuYV and were distinct from Beet western yellows virus (BWYV isolates.

  13. Transgene expression and fitness of hybrids between GM oilseed rape and Brassica rapa.

    Science.gov (United States)

    Ammitzbøll, Henriette; Mikkelsen, Teis Nørgaard; Jørgensen, Rikke Bagger

    2005-01-01

    Oilseed rape (Brassica napus) is sexually compatible with its wild and weedy relative B. rapa, and introgression of genes from B. napus has been found to occur over a few generations. We simulated the early stages of transgene escape by producing F1 hybrids and the first backcross generation between two lines of transgenic B. napus and two populations of weedy B. rapa. Transgene expression and the fitness of the hybrids were examined under different environmental conditions. Expression of the transgenes was analyzed at the mRNA level by quantitative PCR and found to be stable in the hybrids, regardless of the genetic background and the environment, and equal to the level of transcription in the parental B. napus lines. Vigor of the hybrids was measured as the photosynthetic capability; pollen viability and seed set per silique. Photosynthetic capability of first generation hybrids was found to be at the same level, or higher, than that of the parental species, whereas the reproductive fitness was significantly lower. The first backcross generation had a significantly lower photosynthetic capability and reproductive fitness compared to the parental species. This is the first study that examines transgene expression at the mRNA level in transgenic hybrids of B. napus of different genetic background exposed to different environmental conditions. The data presented clarify important details of the overall risk assessment of growing transgenic oilseed rape.

  14. Regulation of erucic acid accumulation in oilseed rape (Brassica napus L.) : effects of temperature and abscisic acid

    NARCIS (Netherlands)

    Wilmer, J.A.

    1997-01-01

    Vegetable oils are an important commodity world-wide with an annual production of about 70 million tonnes. Oilseed rape is one of the four major crops, providing about 10% of the total production. Quality of vegetable oils is determined by the fatty acid composition of the triacylglycerols

  15. FEASIBILITY OF HYGROMYCIN AS A SELECTION AGENT IN AGROBACTERIUM-MEDIATED TRANSFORMATION OF OILSEED RAPE (BRASSICA NAPUS L.

    Directory of Open Access Journals (Sweden)

    Tímea Kuťka Hlozáková

    2014-02-01

    Full Text Available In this work the feasibility of the antibiotic hygromycin as a selection agent in Agrobacterium-mediated transformation of oilseed rape (Brassica napus L. was evaluated. For this, two economically important commercial varieties Haydn and Hunter and tobacco as a model plant were subjected to Agrobacterium-mediated transformation. The 5-6 days-old oilseed rape hypocotyls and 4-6 weeks-old tobacco leaf segments were transformed with the binary vector pCambia1304. The T-DNA contained the reporter gfp:gus and the selectable marker htp genes. Regeneration of transformed cells was conducted under selection of 10 mg.l-1 (oilseed rape and 30 mg.l-1 (tobacco hygromycin. Putative transgenic plantlets were analysed by the mean of the histochemical GUS and PCR analyses. Transformation efficiency ranged from 1.0% (cv. Haydn to 40.4% (tobacco. No transgenic shoots were detected for the cv. Hunter. It points out the oilseed rape cultivar specificity plays significant role in choice of suitable selection agent.

  16. Occurrence of Plasmodiophora brassicae in Finnish turnip rape and oilseed rape fields

    Directory of Open Access Journals (Sweden)

    Marika Rastas

    2012-06-01

    Full Text Available Clubroot, caused by Plasmodiophora brassicae Woronin, is a serious plant disease of cruciferous plants. A field survey of occurrence of clubroot in oilseed fields was conducted in Finland in 2007−2009 and in 1984−1989. At present, the disease is distributed throughout the oilseed cultivation area. Clubroot was found on average from 30% of fields, but its severity was low; fields with high numbers of infected plants and plants with severe symptoms were rare. According to the survey, cultivation frequency of cruciferous plants is the most important factor affecting clubroot occurrence and severity. Clubroot was found in soils with a wide range of pH-values (pH 5-7.6, but symptoms were most severe at low pH. According to the survey, and greenhouse and field trials, high temperature and moisture during the early growth period seem to favour disease development and can cause significant yield losses. In a survival trial, clubroot declined to close to zero after four years in the absence of host plants, but traces of the pathogen were still detectable after a 19-year trial period, making eradication of the pathogen very difficult.

  17. Growth and Yield Stimulation of Winter Oilseed Rape (Brasssica Napus L. by Mg-Titanit Fertiliser

    Directory of Open Access Journals (Sweden)

    Kováčik Peter

    2014-12-01

    Full Text Available The effort to achieve higher yields at the required qualitative level has led to the intensive studying the problems of the rational usage of the titanium containing fertilisers by the agricultural research in the course the previous thirty years. Therefore, the objective of the experiment was to evaluate the impact of two doses of Mg-Titanit (0.2 l/ha and 0.4 l/ha on the formation of the aboveground and underground phytomass, also on the total chlorophyll in leaves, on the titanium and nitrogen content in the seeds and straw, on the quantity and quality of winter rape yield. The doses were applied two or three times on the leaves of winter rape (BBCH 50-52, BBCH 59, BBCH 66-67. The experiment was realized on the Haplic Chernozem (48°42´ N, 17°70´ E - Western Slovakia during two farming years (2009/2010 and 2010/2011. The results showed that all three applications of Mg-Titanit in both doses stimulated the formation of aboveground and underground phytomass of winter rape. The highest growth of phytomass was detected after the second application. The application of Mg-Titanit in the growth phases BBCH 50-52 and BBCH 59 increased the contents of both chlorophylls (a and b, where the growth of chlorophyll b content was more considerable than the growth of chlorophyll a content. The third spraying by Mg-Titanit decreased the content of the total chlorophyll. The application of Mg-Titanit had the positive impact on the yield of seeds, straw and fat content in the winter oilseed rape seeds.

  18. Biology and harmfulness of Brassica pod midge (Dasineura brassicae Winn. in winter oilseed rape

    Directory of Open Access Journals (Sweden)

    Draga Graora

    2015-04-01

    Full Text Available The Brassica pod midge (Dasineura brassicae Winn. is an important pest in oilseed rape (Brasica napus L.. It develops two generations per year and overwinters in the larval stage in cocoons in soil. Immigration of the first generation adults lasted from the beginning of April until the end of May. Larvae developed in pods from mid-April to mid-June, causing pod deformation and cracking, which resulted in premature falling out of seeds and yield reduction. Pod damage amounted to 11.6%. The emergence of the second generation adults was detected at the end of May and in the first ten days of June. D. brassicae was found to lay eggs in healthy pods and no correlation was found with the cabbage seed weevil, Ceutorhynchus assimilis Paykull.

  19. The presence of Bt-transgenic oilseed rape in wild mustard populations affects plant growth.

    Science.gov (United States)

    Liu, Yongbo; Stewart, C Neal; Li, Junsheng; Huang, Hai; Zhang, Xitao

    2015-12-01

    The adventitious presence of transgenic plants in wild plant populations is of ecological and regulatory concern, but the consequences of adventitious presence are not well understood. Here, we introduced Bacillus thuringiensis Cry1Ac (Bt)-transgenic oilseed rape (Bt OSR, Brassica napus) with various frequencies into wild mustard (Brassica juncea) populations. We sought to better understand the adventitious presence of this transgenic insecticidal crop in a wild-relative plant population. We assessed the factors of competition, resource availability and diamondback moth (Plutella xylostella) infestation on plant population dynamics. As expected, Bt OSR performed better than wild mustard in mixed populations under herbivore attack in habitats with enough resources, whereas wild mustard had higher fitness when Bt OSR was rarer in habitats with limited resources. Results suggest that the presence of insect-resistant transgenic plants could decrease the growth of wild mustard and Bt OSR plants and their populations, especially under high herbivore pressure.

  20. Solubilization and characterization of diacylglycerol acyltransferase from microspore-derived cultures of oilseed rape.

    Science.gov (United States)

    Little, D; Weselake, R; Pomeroy, K; Furukawa-Stoffer, T; Bagu, J

    1994-12-15

    Particulate fractions prepared from microspore-derived (MD) embryos of oilseed rape (Brassica napus L. cv. Reston) and an embryogenic MD cell suspension culture of oilseed rape (B. napus L. cv. Jet Neuf) were used as a source of diacylglycerol acyltransferase (DGAT, EC 2.3.1.20) for enzyme characterization and development of a solubilization procedure. DGAT activity in the 1500-100,000 g fraction from MD embryos was stimulated 4-5-fold by 3 to 4 mg of BSA/ml of reaction mixture. DGAT activity from MD embryos was stimulated 2-3-fold by fluoride salts and 1.4-fold by NaCl, whereas iodide salts caused substantial inhibition of enzyme activity. The effect of the various 1:1 electrolytes on enzyme activity appeared to be related more to their differential effects on solution structure rather than ionic strength. DGAT was solubilized from membranes of MD embryos and the cell suspension culture by about 80 and 50% respectively, using 2 M NaCl in 1% (w/v) octanoyl-N-methyl-glucamide (MEGA-8) (pH 8.0 buffer) at a detergent to protein ratio of 2:1. The specific activity of solubilized DGAT was about 2-fold greater than that of the particulate enzyme. The mechanism of solubilization appeared to be related to the lowering of the critical micellar concentration of MEGA-8 in the presence of NaCl. DGAT, solubilized from MD embryos, eluted with an M(r) of about 2 x 10(6) during gel-filtration chromatography on a Superose 6 column equilibrated in buffer containing 0.1% (w/v) MEGA-8. The solubilized enzyme exhibited optimal activity at pH 7. At concentrations above 2 microM acyl-CoA, the specificity of solubilized DGAT for oleoyl-CoA and palmitoyl-CoA was considerably greater than for stearoyl-CoA.

  1. Quantitative Multilevel Analysis of Central Metabolism in Developing Oilseeds of Oilseed Rape during in Vitro Culture1[OPEN

    Science.gov (United States)

    Schwender, Jörg; Hebbelmann, Inga; Heinzel, Nicolas; Hildebrandt, Tatjana; Rogers, Alistair; Klapperstück, Matthias; Schreiber, Falk; Borisjuk, Ljudmilla; Rolletschek, Hardy

    2015-01-01

    Seeds provide the basis for many food, feed, and fuel products. Continued increases in seed yield, composition, and quality require an improved understanding of how the developing seed converts carbon and nitrogen supplies into storage. Current knowledge of this process is often based on the premise that transcriptional regulation directly translates via enzyme concentration into flux. In an attempt to highlight metabolic control, we explore genotypic differences in carbon partitioning for in vitro cultured developing embryos of oilseed rape (Brassica napus). We determined biomass composition as well as 79 net fluxes, the levels of 77 metabolites, and 26 enzyme activities with specific focus on central metabolism in nine selected germplasm accessions. Overall, we observed a tradeoff between the biomass component fractions of lipid and starch. With increasing lipid content over the spectrum of genotypes, plastidic fatty acid synthesis and glycolytic flux increased concomitantly, while glycolytic intermediates decreased. The lipid/starch tradeoff was not reflected at the proteome level, pointing to the significance of (posttranslational) metabolic control. Enzyme activity/flux and metabolite/flux correlations suggest that plastidic pyruvate kinase exerts flux control and that the lipid/starch tradeoff is most likely mediated by allosteric feedback regulation of phosphofructokinase and ADP-glucose pyrophosphorylase. Quantitative data were also used to calculate in vivo mass action ratios, reaction equilibria, and metabolite turnover times. Compounds like cyclic 3′,5′-AMP and sucrose-6-phosphate were identified to potentially be involved in so far unknown mechanisms of metabolic control. This study provides a rich source of quantitative data for those studying central metabolism. PMID:25944824

  2. Intercropping frost-sensitive legume crops with winter oilseed rape reduces weed competition, insect damage, and improves nitrogen use efficiency

    Directory of Open Access Journals (Sweden)

    Cadoux Stéphane

    2015-05-01

    Full Text Available Mixing plant species in agroecosystems is highlighted as an agroecological solution to reduce pesticides and fertilizers while maintaining profitability. In the French context, intercropping frost-sensitive legume crops with winter oilseed rape is potentially interesting and began to be implemented by farmers. In this study we aimed at measuring the services and disservices of this intercrop with three different legume mixtures, in terms of growth and yield for rapeseed, ground cover of weeds in autumn and damage caused by rape winter stem weevil. The experiment was carried out at four sites from 2011 to 2014. We showed higher total aerial dry weights and total aerial nitrogen contents in the intercrops compared to sole winter oilseed rape in November. The companion plants contributed to the control of weeds and the mitigation of rape winter stem weevil damage, notably through the increase in the total aerial weight. In spring, after destruction of the companion plants, the intercrops had partially compensated a reduction in the N fertilization rate (–30 kg per hectare in terms of aerial nitrogen content in rapeseed, with no consequences on the yield which was maintained or even increased. There were probably other interactions such as an improvement in rapeseed root exploration. The consequences were an increase in the nitrogen use efficiency in intercrops. The intercrop with faba bean and lentil showed the best results in terms of autumn growth, weed control, reduction in rape winter stem weevil damage, and rapeseed N content in spring and yield. Intercropping frost-sensitive legume crops with winter oilseed rape is thus a promising way to reconcile yield and reduction in pesticides and fertilizer use and perhaps to benefit more widely to the cropping system.

  3. Adventitious presence of other varieties in oilseed rape (¤Brassica napus¤) from seed banks and certified seed

    DEFF Research Database (Denmark)

    Jørgensen, T.; Hauser, Thure Pavlo; Bagger Jørgensen, Rikke

    2007-01-01

    in the soil seed-bank. This information was acquired through DNA analysis of volunteers collected in the field and seedlings derived from the soil seed-bank. DNA profiles of the volunteers and seedlings were obtained using Inter Simple Sequence Repeat (ISSR) markers, and the profiles were compared with ISSR...... profiles from an assortment of 14 of the most commonly cultivated oilseed rape varieties from 1985 to 2004. This comparison was performed using the assignment program, AFLPOP. The age of the seed bank germinating to become volunteers was assumed from information on previously cultivated oilseed rape...... analysed and, from soil cores, their soil seed-bank was estimated to be 50-100 seeds m(-2). The ISSR analysis of the 14 reference varieties showed that three of the certified seed lots contained other varieties above the allowed threshold. Our findings are discussed in the context of the coexistence...

  4. Mapping Above-Ground Biomass of Winter Oilseed Rape Using High Spatial Resolution Satellite Data at Parcel Scale under Waterlogging Conditions

    Directory of Open Access Journals (Sweden)

    Jiahui Han

    2017-03-01

    Full Text Available Oilseed rape (Brassica napus L. is one of the three most important oil crops in China, and is regarded as a drought-tolerant oilseed crop. However, it is commonly sensitive to waterlogging, which usually refers to an adverse environment that limits crop development. Moreover, crop growth and soil irrigation can be monitored at a regional level using remote sensing data. High spatial resolution optical satellite sensors are very useful to capture and resist unfavorable field conditions at the sub-field scale. In this study, four different optical sensors, i.e., Pleiades-1A, Worldview-2, Worldview-3, and SPOT-6, were used to estimate the dry above-ground biomass (AGB of oilseed rape and track the seasonal growth dynamics. In addition, three different soil water content field experiments were carried out at different oilseed rape growth stages from November 2014 to May 2015 in Northern Zhejiang province, China. As a significant indicator of crop productivity, AGB was measured during the seasonal growth stages of the oilseed rape at the experimental plots. Several representative vegetation indices (VIs obtained from multiple satellite sensors were compared with the simultaneously-collected oilseed rape AGB. Results showed that the estimation model using the normalized difference vegetation index (NDVI with a power regression model performed best through the seasonal growth dynamics, with the highest coefficient of determination (R2 = 0.77, the smallest root mean square error (RMSE = 104.64 g/m2, and the relative RMSE (rRMSE = 21%. It is concluded that the use of selected VIs and high spatial multiple satellite data can significantly estimate AGB during the winter oilseed rape growth stages, and can be applied to map the variability of winter oilseed rape at the sub-field level under different waterlogging conditions, which is very promising in the application of agricultural irrigation and precision agriculture.

  5. Genetic variants associated with the root system architecture of oilseed rape (Brassica napus L.) under contrasting phosphate supply

    OpenAIRE

    Wang, Xiaohua; Chen, Yanling; Thomas, Catherine L.; Ding, Guangda; Xu, Ping; Shi, Dexu; Grandke, Fabian; Jin, Kemo; Cai, Hongmei; Xu, Fangsen; Yi, Bin; Broadley, Martin R.; Shi, Lei

    2017-01-01

    Breeding crops with ideal root system architecture for efficient absorption of phosphorus is an important strategy to reduce the use of phosphate fertilizers. To investigate genetic variants leading to changes in root system architecture, 405 oilseed rape cultivars were genotyped with a 60K Brassica Infinium SNP array in low and high P environments. A total of 285 single-nucleotide polymorphisms were associated with root system architecture traits at varying phosphorus levels. Nine single-nuc...

  6. Mid-infrared spectroscopy combined with chemometrics to detect Sclerotinia stem rot on oilseed rape (Brassica napus L.) leaves.

    Science.gov (United States)

    Zhang, Chu; Feng, Xuping; Wang, Jian; Liu, Fei; He, Yong; Zhou, Weijun

    2017-01-01

    Detection of plant diseases in a fast and simple way is crucial for timely disease control. Conventionally, plant diseases are accurately identified by DNA, RNA or serology based methods which are time consuming, complex and expensive. Mid-infrared spectroscopy is a promising technique that simplifies the detection procedure for the disease. Mid-infrared spectroscopy was used to identify the spectral differences between healthy and infected oilseed rape leaves. Two different sample sets from two experiments were used to explore and validate the feasibility of using mid-infrared spectroscopy in detecting Sclerotinia stem rot (SSR) on oilseed rape leaves. The average mid-infrared spectra showed differences between healthy and infected leaves, and the differences varied among different sample sets. Optimal wavenumbers for the 2 sample sets selected by the second derivative spectra were similar, indicating the efficacy of selecting optimal wavenumbers. Chemometric methods were further used to quantitatively detect the oilseed rape leaves infected by SSR, including the partial least squares-discriminant analysis, support vector machine and extreme learning machine. The discriminant models using the full spectra and the optimal wavenumbers of the 2 sample sets were effective for classification accuracies over 80%. The discriminant results for the 2 sample sets varied due to variations in the samples. The use of two sample sets proved and validated the feasibility of using mid-infrared spectroscopy and chemometric methods for detecting SSR on oilseed rape leaves. The similarities among the selected optimal wavenumbers in different sample sets made it feasible to simplify the models and build practical models. Mid-infrared spectroscopy is a reliable and promising technique for SSR control. This study helps in developing practical application of using mid-infrared spectroscopy combined with chemometrics to detect plant disease.

  7. The effect of water and salt stresses on the phosphorus content and acid phosphatase activity in oilseed rape

    Directory of Open Access Journals (Sweden)

    Stanisław Flasiński

    2014-01-01

    Full Text Available Oilseed rape plants responded to water and salt stresses (-0.5 MPa, PEG 6000 and NaCI by reduction of the fresh and dry weights of shoots and roots. When PEG was used, the ratio of dry weights of roots:shoots surpassed that of controls. The leaf protein content increased considerably. The phosphorus content decreased only in the roots, most significantly after three days of stress. Immediately after the stresses were induced, an increase in the acid phosphatase (AP activity was noted. Water and salt stresses caused four- and two-fold increases in AP activity in leaves, respectively. Changes in the enzyme activity were negligible in stems and roots. There are nine forms of AP in young leaves of oilseed rape. In the stressed plants, from No. 5 revealed lower activity and forms Nos 8 and 9, higher activities than in the control. The increase in AP activity was directly accompanied by the decrease in the water potential of the tissues. Oilseed rape is considerably less sensitive to salt stress than to water stress, which is manifested as the lower inhibition of plant growth and also by a smaller increase in acid phosphatase activity.

  8. Evaluation of nine genotypes of oilseed rape (Brassica napus L.) for larval infestation and performance of rape stem weevil (Ceutorhynchus napi Gyll.).

    Science.gov (United States)

    Schaefer, Heike L; Brandes, Haiko; Ulber, Bernd; Becker, Heiko C; Vidal, Stefan

    2017-01-01

    The rape stem weevil, Ceutorhynchus napi Gyll., is a serious pest of winter oilseed rape (Brassica napus L.) crops in Europe causing severe yield loss. In currently used oilseed rape cultivars no resistance to C. napi has been identified. Resynthesized lines of B. napus have potential to broaden the genetic variability and may improve resistance to insect pests. In this study, the susceptibility to C. napi of three cultivars, one breeding line and five resynthesized lines of oilseed rape was compared in a semi-field plot experiment under multi-choice conditions. Plant acceptance for oviposition was estimated by counting the number of C. napi larvae in stems. The larval instar index and the dry body mass were assessed as indicators of larval performance. The extent of larval feeding within stems was determined by the stem injury coefficient. Morphological stem traits and stem contents of glucosinolates were assessed as potential mediators of resistance. The resynthesized line S30 had significantly fewer larvae than the cultivars Express617 and Visby and the resynthesized lines L122 and L16. The low level of larval infestation in S30 was associated with a low larval instar and stem injury index. Low numbers of larvae were not correlated with the length or diameter of stems, and the level of stem glucosinolates. As indicated by the low larval infestation and slow larval development the resistance of S30 to C. napi is based on both antixenotic and antibiotic properties of the genotypes. The resynthesized line S30 should therefore be introduced into B. napus breeding programs to enhance resistance against C. napi.

  9. Evaluation of nine genotypes of oilseed rape (Brassica napus L. for larval infestation and performance of rape stem weevil (Ceutorhynchus napi Gyll..

    Directory of Open Access Journals (Sweden)

    Heike L Schaefer

    Full Text Available The rape stem weevil, Ceutorhynchus napi Gyll., is a serious pest of winter oilseed rape (Brassica napus L. crops in Europe causing severe yield loss. In currently used oilseed rape cultivars no resistance to C. napi has been identified. Resynthesized lines of B. napus have potential to broaden the genetic variability and may improve resistance to insect pests. In this study, the susceptibility to C. napi of three cultivars, one breeding line and five resynthesized lines of oilseed rape was compared in a semi-field plot experiment under multi-choice conditions. Plant acceptance for oviposition was estimated by counting the number of C. napi larvae in stems. The larval instar index and the dry body mass were assessed as indicators of larval performance. The extent of larval feeding within stems was determined by the stem injury coefficient. Morphological stem traits and stem contents of glucosinolates were assessed as potential mediators of resistance. The resynthesized line S30 had significantly fewer larvae than the cultivars Express617 and Visby and the resynthesized lines L122 and L16. The low level of larval infestation in S30 was associated with a low larval instar and stem injury index. Low numbers of larvae were not correlated with the length or diameter of stems, and the level of stem glucosinolates. As indicated by the low larval infestation and slow larval development the resistance of S30 to C. napi is based on both antixenotic and antibiotic properties of the genotypes. The resynthesized line S30 should therefore be introduced into B. napus breeding programs to enhance resistance against C. napi.

  10. Overexpression of BnWRKY33 in oilseed rape enhances resistance to Sclerotinia sclerotiorum.

    Science.gov (United States)

    Wang, Zheng; Fang, Hedi; Chen, Yu; Chen, Keping; Li, Guanying; Gu, Shoulai; Tan, Xiaoli

    2014-09-01

    Sclerotinia sclerotiorum causes a devastating disease in oilseed rape (Brassica napus) resulting in a tremendous yield loss worldwide. Studies on various host-pathogen interactions have shown that plant WRKY transcription factors are essential for defence. For the B. napus-S. sclerotiorum interaction, little direct evidence has been found with regard to the biological roles of specific WRKY genes in host resistance. In this study, we isolated a B. napus WRKY gene, BnWRKY33, and found that the gene is highly responsive to S. sclerotiorum infection. Transgenic B. napus plants overexpressing BnWRKY33 showed markedly enhanced resistance to S. sclerotiorum, constitutive activation of the expression of BnPR1 and BnPDF1.2, and inhibition of H2 O2 accumulation in response to pathogen infection. Further, we isolated a mitogen-activated protein (MAP) kinase substrate gene, BnMKS1, and found that not only can BnWRKY33 interact with BnMKS1, which can also interact with BnMPK4, using the yeast two-hybrid assay, consistent with their collective nuclear localization, but also BnWRKY33, BnMKS1 and BnMPK4 are substantially and synergistically expressed in response to S. sclerotiorum infection. In contrast, the three genes showed differential expression in response to phytohormone treatments. Together, these results suggest that BnWRKY33 plays an important role in B. napus defence to S. sclerotiorum, which is most probably associated with the activation of the salicylic acid (SA)- and jasmonic acid (JA)-mediated defence response and inhibition of H2 O2 accumulation, and we propose a potential mechanism in which BnMPK4-BnMKS1-BnWRKY33 exist in a nuclear localized complex to regulate resistance to S. sclerotiorum in oilseed rape. © 2014 BSPP AND JOHN WILEY & SONS LTD.

  11. Coordinate changes in gene expression and triacylglycerol composition in the developing seeds of oilseed rape (Brassica napus) and turnip rape (Brassica rapa).

    Science.gov (United States)

    Vuorinen, Anssi L; Kalpio, Marika; Linderborg, Kaisa M; Kortesniemi, Maaria; Lehto, Kirsi; Niemi, Jarmo; Yang, Baoru; Kallio, Heikki P

    2014-02-15

    Crop production for vegetable oil in the northern latitudes utilises oilseed rape (Brassica napus subsp. oleifera) and turnip rape (B. rapa subsp. oleifera), having similar oil compositions. The oil consists mostly of triacylglycerols, which are synthesised during seed development. In this study, we characterised the oil composition and the expression levels of genes involved in triacylglycerol biosynthesis in the developing seeds in optimal, low temperature (15 °C) and short day (12-h day length) conditions. Gene expression levels of several genes were altered during seed development. Low temperature and short day treatments increased the level of 9,12,15-octadecatrienoic acid (18:3n-3) in turnip rape and short day treatment decreased the total oil content in both species. This study gives a novel view on seed oil biosynthesis under different growth conditions, bringing together gene expression levels of the triacylglycerol biosynthesis pathway and oil composition over a time series in two related oilseed species. Copyright © 2013 Elsevier Ltd. All rights reserved.

  12. Hydrogen sulfide alleviates lead-induced photosynthetic and ultrastructural changes in oilseed rape.

    Science.gov (United States)

    Ali, B; Song, W J; Hu, W Z; Luo, X N; Gill, R A; Wang, J; Zhou, W J

    2014-04-01

    The role of hydrogen sulfide (H2S) in alleviating lead (Pb) induced stress in oilseed rape (Brassica napus L.) was studied under laboratory conditions. Plants were grown hydroponically in greenhouse conditions under three levels (0, 100, and 400 µM) of Pb and three levels (0, 100 and 200 µM) of H2S donor, sodium hydrosulfide (NaHS). Application of H2S significantly improved the plant growth, root morphology, chlorophyll contents and photosynthetic activity in leaves of B. napus under Pb stress. Moreover, exogenously applied H2S significantly lowered the Pb concentration in shoots and roots of plants under Pb stress. The microscopic examination indicated that application of exogenous H2S enabled a clean mesophyll cell having a well developed chloroplast with thylakoid membranes and starch grains. A number of modifications could be observed in root tip cell i.e. mature mitochondria, long endoplasmic reticulum and golgibodies under combined application of H2S and Pb. On the basis of these findings, it can be concluded that application of exogenous H2S has a protective role on plant growth, net photosynthesis rate and ultrastructural changes in B. napus plants under high Pb exposures. Copyright © 2014 Elsevier Inc. All rights reserved.

  13. Evaluating agroclimatic constraints and yield gaps for winter oilseed rape (Brassica napus L.) - A case study.

    Science.gov (United States)

    Zhang, Zhi; Lu, Jianwei; Cong, Rihuan; Ren, Tao; Li, Xiaokun

    2017-08-10

    Evaluating the effects of agroclimatic constraints on winter oilseed rape (WOSR) yield can facilitate the development of agricultural mitigation and adaptation strategies. In this study, we investigated the relationship between the WOSR yield and agroclimatic factors using the yield data collected from Agricultural Yearbook and field experimental sites, and the climate dataset from the meteorological stations in Hubei province, China. Five agroclimatic indicators during WOSR growth, such as ≥0 °C accumulated temperature (AT-0), overwintering days (OWD), precipitation (P), precipitation at an earlier stage (EP) and sunshine hours (S), were extracted from twelve agroclimatic indices. The attainable yield for the five yield-limiting factors ranged from 2638 kg ha(-1) (EP) to 3089 kg ha(-1) (AT-0). Farmers (Y farm ) and local agronomists (Y exp ) have achieved 63% and 86% of the attainable yield (Y att ), respectively. The contribution of optimum fertilization to narrow the yield gap (NY exp ) was 52% for the factor P, which was remarkably lower than the mean value (63%). Overall, the precipitation was the crucial yield-limiting agroclimatic factor, and restricted the effect of optimizing fertilization. The integrated data suggest that agricultural strategies of mitigation and adaptation to climatic variability based on different agroclimatic factors are essential for improving the crop yield.

  14. Evidence for pollinator cost and farming benefits of neonicotinoid seed coatings on oilseed rape

    Science.gov (United States)

    Budge, G. E.; Garthwaite, D.; Crowe, A.; Boatman, N. D.; Delaplane, K. S.; Brown, M. A.; Thygesen, H. H.; Pietravalle, S.

    2015-08-01

    Chronic exposure to neonicotinoid insecticides has been linked to reduced survival of pollinating insects at both the individual and colony level, but so far only experimentally. Analyses of large-scale datasets to investigate the real-world links between the use of neonicotinoids and pollinator mortality are lacking. Moreover, the impacts of neonicotinoid seed coatings in reducing subsequent applications of foliar insecticide sprays and increasing crop yield are not known, despite the supposed benefits of this practice driving widespread use. Here, we combine large-scale pesticide usage and yield observations from oilseed rape with those detailing honey bee colony losses over an 11 year period, and reveal a correlation between honey bee colony losses and national-scale imidacloprid (a neonicotinoid) usage patterns across England and Wales. We also provide the first evidence that farmers who use neonicotinoid seed coatings reduce the number of subsequent applications of foliar insecticide sprays and may derive an economic return. Our results inform the societal discussion on the pollinator costs and farming benefits of prophylactic neonicotinoid usage on a mass flowering crop.

  15. Does pea lectin expressed transgenically in oilseed rape (Brassica napus) influence honey bee (Apis mellifera) larvae?

    Science.gov (United States)

    Lehrman, Anna

    2007-01-01

    The European honey bee (Apis mellifera) is important both for pollination and for honey production. Pollen is the major protein source for bees, which exposes them directly to changes in pollen quality e.g. through genetic engineering. In order to create a worst case scenario regarding pea lectin (PSL) expressed transgenically in oilseed rape anthers and pollen, the maximum amount of dried pollen that could be mixed in an artificial diet without negatively affecting larval performance (1.5% w/w) was fed to bee larvae. Pollen from two transgenic plant lines expressing PSL up to 1.2% of total soluble protein and pollen from one non-transgenic line was added to the same diet and used as a pollen control. When these three pollen diets and the control diet (without added pollen) were compared, no negative effect from the pollen of the transgenic plants could be detected on larval mortality, weight, or development time. An increased weight and a reduced developmental time were recorded for larvae on all diets containing pollen when compared to the diet without pollen.

  16. Evaluating the Role of Seed Treatments in Canola/Oilseed Rape Production: Integrated Pest Management, Pollinator Health, and Biodiversity

    Directory of Open Access Journals (Sweden)

    Gregory Sekulic

    2016-08-01

    Full Text Available The use patterns and role of insecticide seed treatments, with focus on neonicotinoid insecticides, were examined for canola/oilseed rape production in Canada and the EU. Since nearly all planted canola acres in Western Canada and, historically, a majority of planted oilseed acres in the EU, use seed treatments, it is worth examining whether broad use of insecticidal seed treatments (IST is compatible with principles of integrated pest management (IPM. The neonicotinoid insecticide (NNI seed treatment (NNI ST use pattern has risen due to effective control of several early season insect pests, the most destructive being flea beetles (Phyllotreta sp.. Negative environmental impact and poor efficacy of foliar applied insecticides on flea beetles led growers to look for better alternatives. Due to their biology, predictive models have been difficult to develop for flea beetles, and, therefore, targeted application of seed treatments, as part of an IPM program, has contributed to grower profitability and overall pollinator success for canola production in Western Canada. Early evidence suggests that the recent restriction on NNI may negatively impact grower profitability and does not appear to be having positive impact on pollinator health. Further investigation on impact of NNI on individual bee vs. hive health need to be conducted. Predictive models for flea beetle emergence/feeding activity in canola/oilseed rape need to be developed, as broad acre deployment of NNI seed treatments may not be sustainable due to concerns about resistance/tolerance in flea beetles and other pest species.

  17. Analysis of yield and plant traits of oilseed rape (Brassica napus L. cultivated in temperate region in light of the possibilities of sowing in arid areas

    Directory of Open Access Journals (Sweden)

    Tadeusz Zając

    2016-12-01

    Full Text Available This work is a review of selected literature on the species of Brassica with the greatest economic significance. Oilseed rape (Brassica napus ssp. oleifera currently ranks third worldwide among oilseed crops used for oil production and is the most important in the temperate zone. The manifold uses of rape include not only human consumption of oil, but also the use of post-extraction meal to feed livestock as well as industrial applications as a source of bioenergy or cellulose. The improvement in the economic position of rape among crop plants is also due to the doubling of its yield between 1970 and 2009; the average annual increase in seed yield worldwide was 27 kg ha−1 yr−1. The yield level in Europe exceeds the average yields achieved in the world, particularly in Asia. Recently, the cultivation of oilseed rape was started on a relatively large acreage in Iran where the yield amounted 2.1 t ha−1, exceeding the yields of China and India. In Poland, the acreage of oilseed rape cultivation between 1965 and 2013 increased 3–4 times, and during this period the annual increase in seed yield was 29 kg ha−1 yr−1. Under the field conditions of the temperate climate zone, winter oilseed rape yield is mainly determined by agro-climatic conditions during the growing period, the level of nitrogen fertilization, and the production potential of varieties, which is currently highest in hybrids. There is a noticeable tendency of hybrids towards formation of more siliques by individual oilseed plants. Different production categories of plants appear in a rape crop. Semi-dwarf varieties of winter rapeseed are distinguished by greater silique density, particularly on the main shoot. Moreover, these hybrids are characterized by faster growth of the root system, which enables them to take up nitrogen from the soil more efficiently.

  18. Quantification of Plasmodiophora brassicae Using a DNA-Based Soil Test Facilitates Sustainable Oilseed Rape Production

    Directory of Open Access Journals (Sweden)

    Ann-Charlotte Wallenhammar

    2016-04-01

    Full Text Available Outbreaks of clubroot disease caused by the soil-borne obligate parasite Plasmodiophora brassicae are common in oilseed rape (OSR in Sweden. A DNA-based soil testing service that identifies fields where P. brassicae poses a significant risk of clubroot infection is now commercially available. It was applied here in field surveys to monitor the prevalence of P. brassicae DNA in field soils intended for winter OSR production and winter OSR field experiments. In 2013 in Scania, prior to planting, P. brassicae DNA was detected in 60% of 45 fields on 10 of 18 farms. In 2014, P. brassicae DNA was detected in 44% of 59 fields in 14 of 36 farms, in the main winter OSR producing region in southern Sweden. P. brassicae was present indicative of a risk for >10% yield loss with susceptible cultivars (>1300 DNA copies g soil−1 in 47% and 44% of fields in 2013 and 2014 respectively. Furthermore, P. brassicae DNA was indicative of sites at risk of complete crop failure if susceptible cultivars were grown (>50 000 copies g−1 soil in 14% and 8% of fields in 2013 and 2014, respectively. A survey of all fields at Lanna research station in western Sweden showed that P. brassicae was spread throughout the farm, as only three of the fields (20% showed infection levels below the detection limit for P.brassicae DNA, while the level was >50,000 DNA copies g−1 soil in 20% of the fields. Soil-borne spread is of critical importance and soil scraped off footwear showed levels of up to 682 million spores g−1 soil. Soil testing is an important tool for determining the presence of P. brassicae and providing an indication of potential yield loss, e.g., in advisory work on planning for a sustainable OSR crop rotation. This soil test is gaining acceptance as a tool that increases the likelihood of success in precision agriculture and in applied research conducted in commercial oilseed fields and at research stations. The present application highlights the importance of

  19. Production of red-flowered oilseed rape via the ectopic expression of Orychophragmus violaceus OvPAP2.

    Science.gov (United States)

    Fu, Wenqin; Chen, Daozong; Pan, Qi; Li, Fengfeng; Zhao, Zhigang; Ge, Xianhong; Li, Zaiyun

    2018-02-01

    Oilseed rape (Brassica napus L.), which has yellow flowers, is both an important oil crop and a traditional tourism resource in China, whereas the Orychophragmus violaceus, which has purple flowers, likely possesses a candidate gene or genes to alter the flower colour of oilseed rape. A previously established B. napus line has a particular pair of O. violaceus chromosomes (M4) and exhibits slightly red petals. In this study, the transcriptomic analysis of M4, B. napus (H3), and O. violaceus with purple petals (OvP) and with white petals (OvW) revealed that most anthocyanin biosynthesis genes were up-regulated in both M4 and OvP. Read assembly and sequence alignment identified a homolog of AtPAP2 in M4, which produced the O. violaceus transcript (OvPAP2). The overexpression of OvPAP2 via the CaMV35S promoter in Arabidopsis thaliana led to different levels of anthocyanin accumulation in most organs, including the petals. However, the B. napus overexpression plants showed anthocyanin accumulation primarily in the anthers, but not the petals. However, when OvPAP2 was driven by the petal-specific promoter XY355, the transgenic B. napus plants produced red anthers and red petals. The results of metabolomic experiments showed that specific anthocyanins accumulated to high levels in the red petals. This study illustrates the feasibility of producing red-flowered oilseed rape, thereby enhancing its ornamental value, via the ectopic expression of the OvPAP2 gene. Moreover, the practical application of this study for insect pest management in the crop is discussed. © 2017 The Authors. Plant Biotechnology Journal published by Society for Experimental Biology and The Association of Applied Biologists and John Wiley & Sons Ltd.

  20. Milestone – a selective herbicide for the control of important grasses and broadleaved weeds in winter oilseed rape

    Directory of Open Access Journals (Sweden)

    Zotz, Agnes

    2016-02-01

    Full Text Available MilestoneTM herbicide contains the active substance propyzamide (500 g/L and aminopyralid (5.3 g a.e./L and is formulated as a suspension concentrate (SC. Registration for Milestone for the use in winter oilseed rape was granted in Germany in July 2014. The active substance propyzamide is well known from the product KerbTM FLO (containing 500 g/L propyzamide, suspension concentrate, SC which is widely used in winter oilseed rape (WITTROCK et al., 2008. Aminopyralid is formulated in the commercial product Runway (clopyralid + picloram + aminopyralid. Milestone is applied with a use rate of 1.5 L/ha as a post-emergence herbicide from growth stage BBCH 14 of the crop at the beginning of November until February. Kerb FLO is applied with a use rate of 1.875 L/ha at the same timing. The efficacy of Milestone and Kerb FLO was tested in randomised and replicated plot trials in Germany, France and the United Kingdom. Milestone and Kerb FLO showed comparable and very high control levels against monocotyledonous species such as Alopecurus myosuroides, Apera spica-venti, Bromus species and volunteer cereals. Milestone shows a broader spectrum of activity vs. Kerb FLO against dicotyledonous weeds such as Matricaria chamomilla, Papaver rhoeas and Centaurea cyanus. The use of Milestone in dense crops (as the situation was in autumn 2014 for many areas in Germany shows very high efficacy levels as well. The comparison of various application timings between end of October until beginning of December confirms the application date early November for best results. Milestone controls herbicide-resistant weed populations and can be considered an important part of a resistance management program not only in winter oilseed rape but as a component of an integrated weed management strategy in cropping systems.

  1. Effects of experimental warming on fungal disease progress in oilseed rape.

    Science.gov (United States)

    Siebold, Magdalena; von Tiedemann, Andreas

    2013-06-01

    Global warming will influence the growth and development of both crops and pathogens. The aims of this study were to investigate potential effects of future warming on oilseed rape growth and the epidemiology of the three economically important pathogens Verticillium longisporum, Sclerotinia sclerotiorum, and Leptosphaeria maculans (anamorph: Phoma lingam). We utilized climate chambers and a soil warming facility, where treatments represented regional warming scenarios for Lower Saxony, Germany, by 2050 and 2100, and compared results of both approaches on a thermal time scale by calculating degree-days (dd) from day of sowing, December 1st and March 1st until sampling, the latter correlating best with disease progress. Regression analysis showed that plant growth and growth stages in spring responded almost linearly to increasing thermal time until 1000-1500 dd. Colonization of plant tissue by V. longisporum showed an exponential increase when exceeding 1300-1500 dd and reaching plant growth stage BBCH 74/75 (pod development). V. longisporum colonization of plants may be advanced, potentially leading to higher inoculum densities after harvest and increased economic importance of this pathogen under future warming. Sclerotia germination of S. sclerotiorum reached its maximum at 600-900 dd. Advance of these critical degree-days may lead to earlier apothecia production, potentially advancing the infection window, whereas the future importance of S. sclerotiorum may remain constant. Severity of phoma crown canker increased linearly with increasing thermal time, but showed also large variation in response to the warming scenarios, suggesting that factors such as canopy microclimate in fall or leaf shedding over winter may play a bigger role for L. maculans infection and disease severity than higher soil temperatures. Thermal time was a suitable tool to combine and integrate data on biological responses to soil and air temperature increases from climate chamber and field

  2. Consequences of gene flow between oilseed rape (Brassica napus) and its relatives.

    Science.gov (United States)

    Liu, Yongbo; Wei, Wei; Ma, Keping; Li, Junsheng; Liang, Yuyong; Darmency, Henri

    2013-10-01

    Numerous studies have focused on the probability of occurrence of gene flow between transgenic crops and their wild relatives and the likelihood of transgene escape, which should be assessed before the commercial release of transgenic crops. This review paper focuses on this issue for oilseed rape, Brassica napus L., a species that produces huge numbers of pollen grains and seeds. We analyze separately the distinct steps of gene flow: (1) pollen and seeds as vectors of gene flow; (2) spontaneous hybridization; (3) hybrid behavior, fitness cost due to hybridization and mechanisms of introgression; (4) and fitness benefit due to transgenes (e.g. herbicide resistance and Bt toxin). Some physical, biological and molecular means of transgene containment are also described. Although hybrids and first generation progeny are difficult to identify in fields and non-crop habitats, the literature shows that transgenes could readily introgress into Brassica rapa, Brassica juncea and Brassica oleracea, while introgression is expected to be rare with Brassica nigra, Hirschfeldia incana and Raphanus raphanistrum. The hybrids grow well but produce less seed than their wild parent. The difference declines with increasing generations. However, there is large uncertainty about the evolution of chromosome numbers and recombination, and many parameters of life history traits of hybrids and progeny are not determined with satisfactory confidence to build generic models capable to really cover the wide diversity of situations. We show that more studies are needed to strengthen and organize biological knowledge, which is a necessary prerequisite for model simulations to assess the practical and evolutionary outputs of introgression, and to provide guidelines for gene flow management. Copyright © 2013 Elsevier Ireland Ltd. All rights reserved.

  3. Nitrogen Fertilizer Management for Enhancing Crop Productivity and Nitrogen Use Efficiency in a Rice-Oilseed Rape Rotation System in China

    Directory of Open Access Journals (Sweden)

    Muhammad Yousaf

    2016-09-01

    Full Text Available The use of efficient rates of nitrogen (N fertilizer application is important with regard to increasing crop productivity and maintaining environmental sustainability. Rice-oilseed rape rotations are a mainstay of the economy and food security of China. Therefore, a field experiment was carried out during 2011–2013 in Honghu to identify the most appropriate N application rates for enhancing crop productivity and N use efficiency for rice (Oryza sativa L.-oilseed rape (Brassica napus L. rotations. Six N fertilizer treatments (RO1, RO2, RO3, RO4, RO5, and RO6 were laid out in a randomized complete block design with three replicates. ROx represented the N fertilizer application rates (kg ha–1 for rice and oilseed rape, respectively. Grain yields from plots receiving N fertilizer were significantly increased by 59–71% (rice and 109–160% (oilseed rape during the total rotation (2011–2013, as compared to RO1 (control; no application. Furthermore, a similar trend was observed for N accumulation, ranging from 88–125% and 134–200% in aerial parts of rice and oilseed rape, respectively. Nitrogen use efficiency (NUE was significantly higher (38.5% under RO2 and lower (34.2% under RO6 while apparent N balance (ANB was positively lowest under R05 (183.4 kg ha–1 followed by R02 (234.2 kg ha–1 and highest under R06 (344.5 kg ha–1 during the total rotation. The results of grain yield, NUE, and ANB indicated that the R02 rate of N application was superior. This information should help to develop a cost-effective and environment-friendly N management strategy for rice-oilseed rape rotation systems of central China.

  4. The use of Pseudomonas fluorescens P13 to control sclerotinia stem rot (Sclerotinia sclerotiorum) of oilseed rape.

    Science.gov (United States)

    Li, Hui; Li, Huaibo; Bai, Yan; Wang, Jing; Nie, Ming; Li, Bo; Xiao, Ming

    2011-12-01

    Sclerotinia stem rot (SSR) caused by the fungus Sclerotinia sclerotiorum has been an increasing threat to oilseed rape (Brassica napus L.) cultivation. Efficient and environment-friendly treatments are much needed. Here we focus on microbial control. The Pseudomonas fluorescens P13 that was isolated from oilseed rape cultivation soil, proved to be a useful biocontrol strain for application. Morphology, physiological and biochemical tests and 16S rDNA analysis demonstrated that it was P. fluorescens P13 and that it had a broad antagonistic spectrum, significantly lessening the mycelial growth of S. sclerotiorum by 84.4% and suppressing sclerotial formation by 95-100%. Scanning electron microscopy studies attested that P13 deformed S. sclerotiorum mycelia when they were cultured together. P13 did not produce chitinase but did produce hydrogen cyanide (HCN) which was likely one of the antagonistic mechanisms. The density of P13 remained at a high level (≥10(6) CFU/ml) during 5 weeks in the rhizosphere soil and roots. P13 reduced SSR severity at least by 59% in field studies and also promoted seedling growth (psclerotiorum.

  5. Detection and characterization of carbendazim resistance in Sclerotinia sclerotiorum isolates from oilseed rape in Anhui Province of China.

    Science.gov (United States)

    Xu, D; Pan, Y; Zhang, H; Li, X; Dai, Y; Cao, S; Gao, Z

    2015-12-11

    Stem rot caused by Sclerotinia sclerotiorum is a devastating disease of oilseed rape (Brassica napus) in Anhui Province of China. The fungicide carbendazim (methyl benzimidazole-2-yl carbamate; MBC) has been used to control this fungal disease since the 1980s. In the present study, 74 isolates of S. sclerotiorum from 13 regions of Anhui were collected, and the sensitivities of these isolates to MBC were examined to monitor fungicide resistance. We found that 22 of the 74 isolates showed resistance to MBC, indicating that S. sclerotiorum has developed resistance in parts of Anhui Province. PCR assays and DNA sequence analysis showed that isolates with high MBC resistance had a point mutation at position 198 in the β-tubulin gene that caused a glutamic acid-to-alanine change in the protein. The β-tubulin gene in low-resistance isolates did not have the mutation. These results indicate that the mutation in β-tubulin gene may be associated with high MBC resistance in S. sclerotiorum. The present study also found no correlation between MBC resistance and pathogenicity of S. sclerotiorum isolates, suggesting that the pathogenicity of S. sclerotiorum isolates on oilseed rape did not vary with MBC resistance status.

  6. Mapping the mosaic of ancestral genotypes in a cultivar of oilseed rape (Brassica napus) selected via pedigree breeding.

    Science.gov (United States)

    Sharpe, A G; Lydiate, D J

    2003-06-01

    Recent oilseed rape breeding has produced low glucosinolate cultivars that yield proteinaceous meal suitable for animal feed. The low glucosinolate character was introduced into modern cultivars from Brassica napus 'Bronowski', a cultivar that is agronomically inferior in most other respects. Residual segments of 'Bronowski' genotype in modern cultivars probably cause reduced yield, poorer winter hardiness, and lower oil content. The quantity and distribution of the 'Bronowski' genotype in the modern oilseed rape cultivar Brassica napus 'Tapidor' was investigated using a segregating population derived from a cross between 'Tapidor' and its high glucosinolate progenitor. This population was analyzed with 65 informative Brassica RFLP probes and a genetic linkage map, based on the segregation at 77 polymorphic loci, was constructed. The mapping identified 15 residual segments of donor genotype in 'Tapidor', which together occupy approximately 29% of the B. napus genome. Mapping the loci that control variation for the accumulation of total seed glucosinolates in the segregating population has identified three loci that together explain >90% of the variation for this character. All of these loci are in donor segments of the 'Tapidor' genome. This result shows the extent to which conventional breeding programmes have difficulty in eliminating residual segments of donor genotype from elite material.

  7. Temporal Polarimetric Behavior of Oilseed Rape (Brassica napus L. at C-Band for Early Season Sowing Date Monitoring

    Directory of Open Access Journals (Sweden)

    Hao Yang

    2014-10-01

    Full Text Available Spatial monitoring of the sowing date plays an important role in crop yield estimation at the regional scale. The feasibility of using polarimetric synthetic aperture radar (SAR data for early season monitoring of the sowing dates of oilseed rape (Brassica napus L. fields is explored in this paper. Polarimetric SAR responses of six parameters, relying on polarization decomposition methods, were investigated as a function of days after sowing (DAS during the entire growing season, by means of five consecutive Radarsat-2 images. A near-continuous temporal evolution of these parameters was observed, based on 88 oilseed rape fields. It provided a solid basis for determining the suitable temporal window and the best polarimetric parameters for sowing date monitoring. A high sensitivity of all polarimetric parameters to the DAS at different growing stages was shown. Simple linear models could be calibrated to estimate sowing dates at early growth stages and were validated on an independent data set. Although Volume and Span parameters provided the highest sowing date estimation accuracy at the earlier growth stages, the other four parameters (Volume/Total, Odd/Total, Entropy and Alpha were more accurate for a wider temporal window. These results demonstrate the capability and high potential of polarimetric SAR data for monitoring the sowing date of crops in the early season.

  8. Genome-wide analysis and expression profiling of the GRF gene family in oilseed rape (Brassica napus L.).

    Science.gov (United States)

    Ma, Jin-Qi; Jian, Hong-Ju; Yang, Bo; Lu, Kun; Zhang, Ao-Xiang; Liu, Pu; Li, Jia-Na

    2017-07-15

    Growth regulating-factors (GRFs) are plant-specific transcription factors that help regulate plant growth and development. Genome-wide identification and evolutionary analyses of GRF gene families have been performed in Arabidopsis thaliana, Zea mays, Oryza sativa, and Brassica rapa, but a comprehensive analysis of the GRF gene family in oilseed rape (Brassica napus) has not yet been reported. In the current study, we identified 35 members of the BnGRF family in B. napus. We analyzed the chromosomal distribution, phylogenetic relationships (Bayesian Inference and Neighbor Joining method), gene structures, and motifs of the BnGRF family members, as well as the cis-acting regulatory elements in their promoters. We also analyzed the expression patterns of 15 randomly selected BnGRF genes in various tissues and in plant varieties with different harvest indices and gibberellic acid (GA) responses. The expression levels of BnGRFs under GA treatment suggested the presence of possible negative feedback regulation. The evolutionary patterns and expression profiles of BnGRFs uncovered in this study increase our understanding of the important roles played by these genes in oilseed rape. Copyright © 2017. Published by Elsevier B.V.

  9. Introduction of beet cyst nematode resistance from Sinapis alba L. and Raphanus sativus L. into Brassica napus L. (oil-seed rape) through sexual and somatic hybridization

    NARCIS (Netherlands)

    Lelivelt, C.L.C.

    1993-01-01

    Experiments were performed to select for beet cyst nematode (Heterodera schachtii Schm., abbrev. BCN) resistant genotypes of Brassica napus L. (oilseed rape), and to introduce BCN-resistance from the related species Raphanus

  10. Epidemiology of light leaf spot (Pyrenopeziza brassicae) on winter oilseed rape (Brassica napus) = De epidemiologie van 'light leaf spot' (Pyrenopeziza brassicae) in winterkoolzaad (Brassica napus)

    NARCIS (Netherlands)

    Gilles, T.

    2000-01-01

    Forecasts of the severity of light leaf spot of winter oilseed rape are needed to help growers with their decisions on fungicide applications at times when sprays are needed to control light leaf spot, but the disease is difficult to diagnose. A thorough understanding of stages in

  11. Formulations of the endophytic bacterium Bacillus subtilis Tu-100 suppress Sclerotinia sclerotiorum on oilseed rape and improve plant vigor in field trials conducted at separate locations

    Science.gov (United States)

    Sclerotinia sclerotiorum causes serious yield losses in crops in The People’s Republic of China. Two formulations of oilseed rape seed containing the endophytic bacterium Bacillus subtilis Tu-100 were evaluated for suppression of this pathogen in field trials conducted at two independent locations....

  12. Overexpression of sinapine esterase BnSCE3 in oilseed rape seeds triggers global changes in seed metabolism.

    Science.gov (United States)

    Clauss, Kathleen; von Roepenack-Lahaye, Edda; Böttcher, Christoph; Roth, Mary R; Welti, Ruth; Erban, Alexander; Kopka, Joachim; Scheel, Dierk; Milkowski, Carsten; Strack, Dieter

    2011-03-01

    Sinapine (O-sinapoylcholine) is the predominant phenolic compound in a complex group of sinapate esters in seeds of oilseed rape (Brassica napus). Sinapine has antinutritive activity and prevents the use of seed protein for food and feed. A strategy was developed to lower its content in seeds by expressing an enzyme that hydrolyzes sinapine in developing rape seeds. During early stages of seedling development, a sinapine esterase (BnSCE3) hydrolyzes sinapine, releasing choline and sinapate. A portion of choline enters the phospholipid metabolism, and sinapate is routed via 1-O-sinapoyl-β-glucose into sinapoylmalate. Transgenic oilseed rape lines were generated expressing BnSCE3 under the control of a seed-specific promoter. Two distinct single-copy transgene insertion lines were isolated and propagated to generate homozygous lines, which were subjected to comprehensive phenotyping. Sinapine levels of transgenic seeds were less than 5% of wild-type levels, whereas choline levels were increased. Weight, size, and water content of transgenic seeds were significantly higher than those of wild-type seeds. Seed quality parameters, such as fiber and glucosinolate levels, and agronomically important traits, such as oil and protein contents, differed only slightly, except that amounts of hemicellulose and cellulose were about 30% higher in transgenic compared with wild-type seeds. Electron microscopic examination revealed that a fraction of the transgenic seeds had morphological alterations, characterized by large cavities near the embryonic tissue. Transgenic seedlings were larger than wild-type seedlings, and young seedlings exhibited longer hypocotyls. Examination of metabolic profiles of transgenic seeds indicated that besides suppression of sinapine accumulation, there were other dramatic differences in primary and secondary metabolism. Mapping of these changes onto metabolic pathways revealed global effects of the transgenic BnSCE3 expression on seed metabolism.

  13. Large-scale monitoring of effects of clothianidin-dressed oilseed rape seeds on pollinating insects in northern Germany: residues of clothianidin in pollen, nectar and honey.

    Science.gov (United States)

    Rolke, Daniel; Persigehl, Markus; Peters, Britta; Sterk, Guido; Blenau, Wolfgang

    2016-11-01

    This study was part of a large-scale monitoring project to assess the possible effects of Elado(®) (10 g clothianidin & 2 g β-cyfluthrin/kg seed)-dressed oilseed rape seeds on different pollinators in Northern Germany. Firstly, residues of clothianidin and its active metabolites thiazolylnitroguanidine and thiazolylmethylurea were measured in nectar and pollen from Elado(®)-dressed (test site, T) and undressed (reference site, R) oilseed rape collected by honey bees confined within tunnel tents. Clothianidin and its metabolites could not be detected or quantified in samples from R fields. Clothianidin concentrations in samples from T fields were 1.3 ± 0.9 μg/kg and 1.7 ± 0.9 μg/kg in nectar and pollen, respectively. Secondly, pollen and nectar for residue analyses were sampled from free flying honey bees, bumble bees and mason bees, placed at six study locations each in the R and T sites at the start of oilseed rape flowering. Honey samples were analysed from all honey bee colonies at the end of oilseed rape flowering. Neither clothianidin nor its metabolites were detectable or quantifiable in R site samples. Clothianidin concentrations in samples from the T site were below the limit of quantification (LOQ, 1.0 µg/kg) in most pollen and nectar samples collected by bees and 1.4 ± 0.5 µg/kg in honey taken from honey bee colonies. In summary, the study provides reliable semi-field and field data of clothianidin residues in nectar and pollen collected by different bee species in oilseed rape fields under common agricultural conditions.

  14. Genome-wide analysis and expression profiling of the SUC and SWEET gene families of sucrose transporters in oilseed rape (Brassica napus L.

    Directory of Open Access Journals (Sweden)

    JIAN Hongju

    2016-09-01

    Full Text Available Sucrose is the principal transported product of photosynthesis from source leaves to sink organs. SUTs/SUCs (sucrose transporters or sucrose carriers and SWEETs (Sugars Will Eventually be Exported Transporters play significant central roles in phloem loading and unloading. SUTs/SUCs and SWEETs are key players in sucrose translocation and are associated with crop yields. The SUT/SUC and SWEET genes have been characterized in several plant species, but a comprehensive analysis of these two gene families in oilseed rape has not yet been reported. In our study, 22 and 68 members of the SUT/SUCs and SWEET gene families, respectively, were identified in the oilseed rape (Brassica napus genome through homology searches. An analysis of the chromosomal distribution, phylogenetic relationships, gene structures, motifs and the cis-acting regulatory elements in the promoters of BnSUC and BnSWEET genes were analysed. Furthermore, we examined the expression of the 18 BnSUC and 16 BnSWEET genes in different tissues of ‘ZS11’ and the expression of 9 BnSUC and 7 BnSWEET genes in ‘ZS11’ under various conditions, including biotic stress (Sclerotinia sclerotiorum, abiotic stresses (drought, salt and heat, and hormone treatments (abscisic acid, auxin, cytokinin, brassinolide, gibberellin and salicylic acid. In conclusion, our study provides the first comprehensive analysis of the oilseed rape SUC and SWEET gene families. Information regarding the phylogenetic relationships, gene structure and expression profiles of the SUC and SWEET genes in the different tissues of oilseed rape helps to identify candidates with potential roles in specific developmental processes. Our study advances our understanding of the important roles of sucrose transport in oilseed rape.

  15. Rice Yield and the Fate of Fertilizer Nitrogen as Affected by Addition of Earthworm Casts Collected from Oilseed Rape Fields: A Pot Experiment.

    Directory of Open Access Journals (Sweden)

    Min Huang

    Full Text Available The mechanism associated with improvement of soil nutritional status by oilseed rape crop, leading to better performance of rice crop, in rice-oilseed rape cropping systems is little known. The present study was aimed to test the hypothesis that earthworm casts produced during oilseed rape-growing season have positive effects on grain yield and fertilizer nitrogen (N utilization in the subsequent flooded rice crop. A 15N-tracing pot experiment was conducted to determine the effects of earthworm casts collected from oilseed rape fields on yield attributes in rice and the fate of fertilizer N. Soil treated with earthworm casts (soil: earthworm casts = 4: 1, w/w (EC1 produced 39% higher grain yield than soil only (EC0. EC1 had 18% more panicle number and 10% higher spikelet filling percentage than EC0. Aboveground biomass and harvest index were higher in EC1 than in EC0 by 20% and 15%, respectively. SPAD values in flag leaves were 10% and 22% higher under EC1 than EC0 at 15 and 20 days after heading, respectively. EC1 had 19% higher total N uptake and 18% higher physiological N-use efficiency than EC0. These positive effects of earthworm casts on yield attributes offset negative effects of decreasing N rate from 0.74 g pot-1 (equivalent to the recommended field rate of 150 kg ha-1 to 0.44 g pot-1 (equivalent to 60% of the recommended rate. Fertilizer N retention rate was 7% higher while fertilizer N loss rate was 6% lower in EC1 than in EC0. Our study suggests that earthworm casts produced during oilseed rape-growing season are expected to have the following benefits on the subsequent flooded rice system: (1 improving growth and physiological processes in rice plants and consequently increasing rice grain yield, and (2 increasing fertilizer N retention rate and hence decreasing fertilizer N loss rate and reducing environmental risk.

  16. Rice Yield and the Fate of Fertilizer Nitrogen as Affected by Addition of Earthworm Casts Collected from Oilseed Rape Fields: A Pot Experiment.

    Science.gov (United States)

    Huang, Min; Zhou, Xuefeng; Xie, Xiaobing; Zhao, Chunrong; Chen, Jiana; Cao, Fangbo; Zou, Yingbin

    2016-01-01

    The mechanism associated with improvement of soil nutritional status by oilseed rape crop, leading to better performance of rice crop, in rice-oilseed rape cropping systems is little known. The present study was aimed to test the hypothesis that earthworm casts produced during oilseed rape-growing season have positive effects on grain yield and fertilizer nitrogen (N) utilization in the subsequent flooded rice crop. A 15N-tracing pot experiment was conducted to determine the effects of earthworm casts collected from oilseed rape fields on yield attributes in rice and the fate of fertilizer N. Soil treated with earthworm casts (soil: earthworm casts = 4: 1, w/w) (EC1) produced 39% higher grain yield than soil only (EC0). EC1 had 18% more panicle number and 10% higher spikelet filling percentage than EC0. Aboveground biomass and harvest index were higher in EC1 than in EC0 by 20% and 15%, respectively. SPAD values in flag leaves were 10% and 22% higher under EC1 than EC0 at 15 and 20 days after heading, respectively. EC1 had 19% higher total N uptake and 18% higher physiological N-use efficiency than EC0. These positive effects of earthworm casts on yield attributes offset negative effects of decreasing N rate from 0.74 g pot-1 (equivalent to the recommended field rate of 150 kg ha-1) to 0.44 g pot-1 (equivalent to 60% of the recommended rate). Fertilizer N retention rate was 7% higher while fertilizer N loss rate was 6% lower in EC1 than in EC0. Our study suggests that earthworm casts produced during oilseed rape-growing season are expected to have the following benefits on the subsequent flooded rice system: (1) improving growth and physiological processes in rice plants and consequently increasing rice grain yield, and (2) increasing fertilizer N retention rate and hence decreasing fertilizer N loss rate and reducing environmental risk.

  17. Preferential exclusion of hybrids in mixed pollinations between oilseed rape (Brassica napus) and weedy B. campestris (Brassicaceae)

    DEFF Research Database (Denmark)

    Hauser, T.P.; Bagger Jørgensen, Rikke; Østergård, Hanne

    1997-01-01

    In most experimental hybridizations between oilseed rape (Brassica napus) and weedy B. campestris, either intra- or interspecific pollen has been applied to individual flowers. Under field conditions, however, stigmas will often receive a mixture of the two types of pollen, thereby allowing...... for competition between male gametophytes and/or seeds within pods. To test whether competition influences the success of hybridization, pollen from the two species was mixed in different proportions and applied to stigmas of both species. The resulting seeds were scored for paternity by isozyme and randomly...... survival of hybrid zygotes in B. napus pods. This is in contrast to the higher survival of hybrid seeds in B. napus than in B. campestris pods when pollinations are made with pure pollen. Altogether, the likelihood of a foreign pollen grain producing a seed was much lower on B. napus than on B. campestris...

  18. Non-destructive determination of Malondialdehyde (MDA) distribution in oilseed rape leaves by laboratory scale NIR hyperspectral imaging

    Science.gov (United States)

    Kong, Wenwen; Liu, Fei; Zhang, Chu; Zhang, Jianfeng; Feng, Hailin

    2016-10-01

    The feasibility of hyperspectral imaging with 400-1000 nm was investigated to detect malondialdehyde (MDA) content in oilseed rape leaves under herbicide stress. After comparing the performance of different preprocessing methods, linear and nonlinear calibration models, the optimal prediction performance was achieved by extreme learning machine (ELM) model with only 23 wavelengths selected by competitive adaptive reweighted sampling (CARS), and the result was RP = 0.929 and RMSEP = 2.951. Furthermore, MDA distribution map was successfully achieved by partial least squares (PLS) model with CARS. This study indicated that hyperspectral imaging technology provided a fast and nondestructive solution for MDA content detection in plant leaves.

  19. Genotypic Variation in Nitrogen Utilization Efficiency of Oilseed Rape (Brassica napus) Under Contrasting N Supply in Pot and Field Experiments

    Science.gov (United States)

    He, Huiying; Yang, Rui; Li, Yajun; Ma, Aisheng; Cao, Lanqin; Wu, Xiaoming; Chen, Biyun; Tian, Hui; Gao, Yajun

    2017-01-01

    Oilseed rape (Brassica napus) characteristically has high N uptake efficiency and low N utilization efficiency (NUtE, seed yield/shoot N accumulation). Determining the NUtE phenotype of various genotypes in different growth conditions is a way of finding target traits to improve oilseed rape NUtE. The aim of this study was to compare oilseed rape genotypes grown on contrasting N supply rates in pot and field experiments to investigate the genotypic variations of NUtE and to identify indicators of N efficient genotypes. For 50 oilseed rape genotypes, NUtE, dry matter and N partitioning, morphological characteristics, and the yield components were investigated under high and low N supplies in a greenhouse pot experiment and a field trial. Although the genotype rankings of NUtE were different between the pot experiment and the field trial, some genotypes performed consistently in both two environments. N-responder, N-nonresponder, N-efficient and N-inefficient genotypes were identified from these genotypes with consistent NUtE. The correlations between the pot experiment and the field trial in NUtE were only 0.34 at high N supplies and no significant correlations were found at low N supplies. However, Pearson coefficient correlation (r) and principal component analysis showed NUtE had similar genetic correlations with other traits across the pot and field experiment. Among the yield components, only seeds per silique showed strong and positive correlations with NUtE under varying N supply in both experiments (r = 0.47**; 0.49**; 0.47**; 0.54**). At high and low N supply, NUtE was positively correlated with seed yield (r = 0.45**; 0.53**; 0.39**; 0.87**), nitrogen harvest index (NHI, r = 0.68**; 0.82**; 0.99**; 0.89**), and harvest index (HI, r = 0.79**; 0.83**; 0.90**; 0.78**) and negatively correlated with biomass distribution to stem and leaf (r = −0.34**; −0.45**; −0.37**; 0.62**), all aboveground plant section N concentration (r from −0.30* to −0

  20. Genotypic Variation in Nitrogen Utilization Efficiency of Oilseed Rape (Brassica napus Under Contrasting N Supply in Pot and Field Experiments

    Directory of Open Access Journals (Sweden)

    Huiying He

    2017-10-01

    Full Text Available Oilseed rape (Brassica napus characteristically has high N uptake efficiency and low N utilization efficiency (NUtE, seed yield/shoot N accumulation. Determining the NUtE phenotype of various genotypes in different growth conditions is a way of finding target traits to improve oilseed rape NUtE. The aim of this study was to compare oilseed rape genotypes grown on contrasting N supply rates in pot and field experiments to investigate the genotypic variations of NUtE and to identify indicators of N efficient genotypes. For 50 oilseed rape genotypes, NUtE, dry matter and N partitioning, morphological characteristics, and the yield components were investigated under high and low N supplies in a greenhouse pot experiment and a field trial. Although the genotype rankings of NUtE were different between the pot experiment and the field trial, some genotypes performed consistently in both two environments. N-responder, N-nonresponder, N-efficient and N-inefficient genotypes were identified from these genotypes with consistent NUtE. The correlations between the pot experiment and the field trial in NUtE were only 0.34 at high N supplies and no significant correlations were found at low N supplies. However, Pearson coefficient correlation (r and principal component analysis showed NUtE had similar genetic correlations with other traits across the pot and field experiment. Among the yield components, only seeds per silique showed strong and positive correlations with NUtE under varying N supply in both experiments (r = 0.47**; 0.49**; 0.47**; 0.54**. At high and low N supply, NUtE was positively correlated with seed yield (r = 0.45**; 0.53**; 0.39**; 0.87**, nitrogen harvest index (NHI, r = 0.68**; 0.82**; 0.99**; 0.89**, and harvest index (HI, r = 0.79**; 0.83**; 0.90**; 0.78** and negatively correlated with biomass distribution to stem and leaf (r = −0.34**; −0.45**; −0.37**; 0.62**, all aboveground plant section N concentration (r from −0.30* to

  1. Significant reductions in oil quality and lipid content of oilseed rape (Brassica napus L.) under climate change

    DEFF Research Database (Denmark)

    Namazkar, Shahla; Egsgaard, Helge; Frenck, Georg

    2015-01-01

    stearic acid, C 18:0. For example we found that in the two factor treatment, where elevated [CO2] and temperature were combined, the essential fatty acid omega-3, C18:3, decreased by 45% and oil content declined 10%.Total losses in fatty acid and oil yields would be even larger, when also considering...... fatty acids was changed for the worse. Facing this outlook, breeding for climate tolerant cultivars seems essential for oil yield and quality. (C) 2015 Published by Elsevier B.V......Despite of the potential importance to food and bioenergy purposes, effects from climate change on plant oil quality have hardly been characterized.On a global basis Brassica napus L., rapeseed or oilseed rape, is the second largest source of vegetable oil after soybean and the predominant oil crop...

  2. Significant reductions in oil quality and lipid content of oilseed rape (Brassica napus L.) under climate change

    DEFF Research Database (Denmark)

    Namazkar, Shahla; Egsgaard, Helge; Frenck, Georg

    2015-01-01

    found that in the two- factor treatment, where elevated [CO2] and temperature were combined, the essential fatty acid omega-3, C18:3, decreased by 45% and oil content declined 10%.Total losses in fatty acid and oil yields would be even larger, when also considering reported reductions in seed biomass....... Facing this outlook, breeding for climate tolerant cultivars seems essential for oil yield and quality.......Despite of the potential importance to food and bioenergy purposes, effects from climate change on plant oil quality have hardly been characterized.On a global basis Brassica napus L., rapeseed or oilseed rape, is the second largest source of vegetable oil after soybean and the predominant oil crop...

  3. Hydroponics versus field lysimeter studies of urea, ammonium and nitrate uptake by oilseed rape (Brassica napus L.).

    Science.gov (United States)

    Arkoun, Mustapha; Sarda, Xavier; Jannin, Laëtitia; Laîné, Philippe; Etienne, Philippe; Garcia-Mina, José-Maria; Yvin, Jean-Claude; Ourry, Alain

    2012-09-01

    N-fertilizer use efficiencies are affected by their chemical composition and suffer from potential N-losses by volatilization. In a field lysimeter experiment, (15)N-labelled fertilizers were used to follow N uptake by Brassica napus L. and assess N-losses by volatilization. Use of urea with NBPT (urease inhibitor) showed the best efficiency with the lowest N losses (8% of N applied compared with 25% with urea alone). Plants receiving ammonium sulphate, had similar yield achieved through a better N mobilization from vegetative tissues to the seeds, despite a lower N uptake resulting from a higher volatilization (43% of applied N). Amounts of (15)N in the plant were also higher when plants were fertilized with ammonium nitrate but N-losses reached 23% of applied N. In parallel, hydroponic experiments showed a deleterious effect of ammonium and urea on the growth of oilseed rape. This was alleviated by the nitrate supply, which was preferentially taken up. B. napus was also characterized by a very low potential for urea uptake. BnDUR3 and BnAMT1, encoding urea and ammonium transporters, were up-regulated by urea, suggesting that urea-grown plants suffered from nitrogen deficiency. The results also suggested a role for nitrate as a signal for the expression of BnDUR3, in addition to its role as a major nutrient. Overall, the results of the hydroponic study showed that urea itself does not contribute significantly to the N nutrition of oilseed rape. Moreover, it may contribute indirectly since a better use efficiency for urea fertilizer, which was further increased by the application of a urease inhibitor, was observed in the lysimeter study.

  4. Detecting the Hormonal Pathways in Oilseed Rape behind Induced Systemic Resistance by Trichoderma harzianum TH12 to Sclerotinia sclerotiorum.

    Science.gov (United States)

    Alkooranee, Jawadayn Talib; Aledan, Tamarah Raad; Ali, Ali Kadhim; Lu, Guangyuan; Zhang, Xuekun; Wu, Jiangsheng; Fu, Chunhua; Li, Maoteng

    2017-01-01

    Plants have the ability to resist pathogen attack after infection or treatment with biotic and abiotic elicitors. In oilseed rape plant Brassica napus AACC and in the artificially synthesized Raphanus alboglabra RRCC, the root-colonizing Trichoderma harzianum TH12 fungus triggers induced systemic resistance (ISR), and its culture filtrate (CF) triggers a systemic acquired resistance (SAR) response against infection by the Sclerotinia sclerotiorum. Salicylic acid (SA) and jasmonic acid/ethylene (JA/ET) are plant hormone signals that play important roles in the regulation of ISR and SAR. In this study, at six different time points (1, 2, 4, 6, 8 and 10 days post-infection [dpi]), six resistance genes were used as markers of signaling pathways: JA/ET signaling used AOC3, PDF1.2 and ERF2 genes, while PR-1, TGA5 and TGA6 genes were used as markers of SA signaling. The results of quantitative real-time polymerase chain reaction (qRT-PCR) showed that AOC3, PDF1.2 and ERF2 expression levels in infected leaves of AACC and RRCC increase at 1 and 2 dpi with S. sclerotiorum or inoculation with TH12. PR-1, TGA5 and TGA6 expression levels increased at 8 and 10 dpi in infected leaves. PR-1, TGA5 and TGA6 expression levels increased early in plants treated with CF in both of the healthy genotypes. Furthermore, induction of SA- and JA/ET-dependent defense decreased disease symptoms in infected leaves at different times. The results suggest that the RRCC genotype exhibits resistance to disease and that the ability of TH12 and its CF to induce systemic resistance in susceptible and resistant oilseed rape genotypes exists. In addition, the results indicate for the first time that in RRCC the SA signaling pathway is involved in resistance to necrotrophic pathogens.

  5. Detecting the Hormonal Pathways in Oilseed Rape behind Induced Systemic Resistance by Trichoderma harzianum TH12 to Sclerotinia sclerotiorum.

    Directory of Open Access Journals (Sweden)

    Jawadayn Talib Alkooranee

    Full Text Available Plants have the ability to resist pathogen attack after infection or treatment with biotic and abiotic elicitors. In oilseed rape plant Brassica napus AACC and in the artificially synthesized Raphanus alboglabra RRCC, the root-colonizing Trichoderma harzianum TH12 fungus triggers induced systemic resistance (ISR, and its culture filtrate (CF triggers a systemic acquired resistance (SAR response against infection by the Sclerotinia sclerotiorum. Salicylic acid (SA and jasmonic acid/ethylene (JA/ET are plant hormone signals that play important roles in the regulation of ISR and SAR. In this study, at six different time points (1, 2, 4, 6, 8 and 10 days post-infection [dpi], six resistance genes were used as markers of signaling pathways: JA/ET signaling used AOC3, PDF1.2 and ERF2 genes, while PR-1, TGA5 and TGA6 genes were used as markers of SA signaling. The results of quantitative real-time polymerase chain reaction (qRT-PCR showed that AOC3, PDF1.2 and ERF2 expression levels in infected leaves of AACC and RRCC increase at 1 and 2 dpi with S. sclerotiorum or inoculation with TH12. PR-1, TGA5 and TGA6 expression levels increased at 8 and 10 dpi in infected leaves. PR-1, TGA5 and TGA6 expression levels increased early in plants treated with CF in both of the healthy genotypes. Furthermore, induction of SA- and JA/ET-dependent defense decreased disease symptoms in infected leaves at different times. The results suggest that the RRCC genotype exhibits resistance to disease and that the ability of TH12 and its CF to induce systemic resistance in susceptible and resistant oilseed rape genotypes exists. In addition, the results indicate for the first time that in RRCC the SA signaling pathway is involved in resistance to necrotrophic pathogens.

  6. [Effects of simulated acid rain on oilseed rape (Brassica napus) physiological characteristics at flowering stage and yield].

    Science.gov (United States)

    Cao, Chun-Xin; Zhou, Qin; Han, Liang-Liang; Zhang, Pei; Jiang, Hai-Dong

    2010-08-01

    A pot experiment was conducted to study the effects of different acidity simulated acid rain on the physiological characteristics at flowering stage and yield of oilseed rape (B. napus cv. Qinyou 9). Comparing with the control (pH 6.0), weak acidity (pH = 4.0-5.0) simulated acid rain stimulated the rape growth to some extent, but had less effects on the plant biomass, leaf chlorophyll content, photosynthetic characteristics, and yield. With the further increase of acid rain acidity, the plant biomass, leaf chlorophyll content, photosynthetic rate, antioxidative enzyme activities, and non-enzyme antioxidant contents all decreased gradually, while the leaf malonyldialdehyde (MDA) content and relative conductivity increased significantly. As the results, the pod number per plant, seed number per pod, seed weight, and actual yield decreased. However, different yield components showed different sensitivity to simulated acid rain. With the increasing acidity of simulated acid rain, the pod number per plant and the seed number per pod decreased significantly, while the seed weight was less affected.

  7. Landscape-scale distribution and persistence of genetically modified oilseed rape (Brassica napus) in Manitoba, Canada.

    Science.gov (United States)

    Knispel, Alexis L; McLachlan, Stéphane M

    2010-01-01

    Genetically modified herbicide-tolerant (GMHT) oilseed rape (OSR; Brassica napus L.) was approved for commercial cultivation in Canada in 1995 and currently represents over 95% of the OSR grown in western Canada. After a decade of widespread cultivation, GMHT volunteers represent an increasing management problem in cultivated fields and are ubiquitous in adjacent ruderal habitats, where they contribute to the spread of transgenes. However, few studies have considered escaped GMHT OSR populations in North America, and even fewer have been conducted at large spatial scales (i.e. landscape scales). In particular, the contribution of landscape structure and large-scale anthropogenic dispersal processes to the persistence and spread of escaped GMHT OSR remains poorly understood. We conducted a multi-year survey of the landscape-scale distribution of escaped OSR plants adjacent to roads and cultivated fields. Our objective was to examine the long-term dynamics of escaped OSR at large spatial scales and to assess the relative importance of landscape and localised factors to the persistence and spread of these plants outside of cultivation. From 2005 to 2007, we surveyed escaped OSR plants along roadsides and field edges at 12 locations in three agricultural landscapes in southern Manitoba where GMHT OSR is widely grown. Data were analysed to examine temporal changes at large spatial scales and to determine factors affecting the distribution of escaped OSR plants in roadside and field edge habitats within agricultural landscapes. Additionally, we assessed the potential for seed dispersal between escaped populations by comparing the relative spatial distribution of roadside and field edge OSR. Densities of escaped OSR fluctuated over space and time in both roadside and field edge habitats, though the proportion of GMHT plants was high (93-100%). Escaped OSR was positively affected by agricultural landscape (indicative of cropping intensity) and by the presence of an

  8. Contribution of nitrogen uptake and retranslocation during reproductive growth to the nitrogen efficiency of winter oilseed-rape cultivars (Brassica napus L.) differing in leaf senescence

    OpenAIRE

    Fabian Koeslin-Findeklee; Horst, Walter J.

    2016-01-01

    Genotypic variation in N efficiency defined as high grain yield under limited nitrogen (N) supply of winter oilseed-rape line-cultivars has been predominantly attributed to N uptake efficiency (NUPT) through maintained N uptake during reproductive growth related to functional stay-green. For investigating the role of stay-green, N retranslocation and N uptake during the reproductive phase for grain yield formation, two line cultivars differing in N starvation-induced leaf senescence were grow...

  9. Differences between winter oilseed rape (Brassica napus L.) cultivars in nitrogen starvation-induced leaf senescence are governed by leaf-inherent rather than root-derived signals

    OpenAIRE

    Koeslin-Findeklee, Fabian; Martin A. Becker; van der Graaff, Eric; Roitsch, Thomas; Horst, Walter J.

    2015-01-01

    Nitrogen (N) efficiency of winter oilseed rape (Brassica napus L.) line-cultivars (cvs.), defined as high grain yield under N limitation, has been primarily attributed to maintained N uptake during reproductive growth (N uptake efficiency) in combination with delayed senescence of the older leaves accompanied with maintained photosynthetic capacity (functional stay-green). However, it is not clear whether genotypic variation in N starvation-induced leaf senescence is due to leaf-inherent fact...

  10. Effects of fungicides on in vitro spore germination and mycelial growth of the phytopathogens Leptosphaeria maculans and L. biglobosa (phoma stem canker of oilseed rape).

    Science.gov (United States)

    Eckert, Maria R; Rossall, Stephen; Selley, Andrew; Fitt, Bruce D L

    2010-04-01

    Phoma stem canker, caused by the coexisting related fungal pathogens Leptosphaeria maculans (Des.) Ces. & de Not and L. biglobosa Shoemaker & H Brun, is a major disease of winter oilseed rape in the UK. Annually, over 90% of UK crops receive at least one foliar application of fungicide, but little is known about the sensitivity of the more damaging L. maculans and the less damaging L. biglobosa to these fungicides. The effects of flusilazole, tebuconazole and Methyl Benzimidazole Carbamate (MBC) fungicides (benomyl and carbendazim) on the germination of ascospores, conidia and germ tube growth of both species were examined. Isolates collected from different oilseed rape crops in England and Wales were assessed for their mycelial growth on fungicide-amended medium, and ED(50) values were calculated. Leptosphaeria maculans and L. biglobosa differed in their sensitivity to fungicides. Conidial germination of L. maculans was more sensitive to these fungicides than that of L. biglobosa. Isolates of L. maculans had smaller ED(50) values for mycelial growth for all fungicides tested than isolates of L. biglobosa. These results suggest that fungicide applications might affect the structure of L. maculans/L. biglobosa populations in UK winter oilseed rape crops.

  11. Senescence-specific alteration of hydrogen peroxide levels in Arabidopsis thaliana and oilseed rape spring variety Brassica napus L. cv. Mozart.

    Science.gov (United States)

    Bieker, Stefan; Riester, Lena; Stahl, Mark; Franzaring, Jürgen; Zentgraf, Ulrike

    2012-08-01

    In order to analyze the signaling function of hydrogen peroxide (H(2)O(2)) production in senescence in more detail, we manipulated intracellular H(2)O(2) levels in Arabidopsis thaliala (L.) Heynh by using the hydrogen-peroxide-sensitive part of the Escherichia coli transcription regulator OxyR, which was directed to the cytoplasm as well as into the peroxisomes. H(2)O(2) levels were lowered and senescence was delayed in both transgenic lines, but OxyR was found to be more effective in the cytoplasm. To transfer this knowledge to crop plants, we analyzed oilseed rape plants Brassica napus L. cv. Mozart for H(2)O(2) and its scavenging enzymes catalase (CAT) and ascorbate peroxidase (APX) during leaf and plant development. H(2)O(2) levels were found to increase during bolting and flowering time, but no increase could be observed in the very late stages of senescence. With increasing H(2)O(2) levels, CAT and APX activities declined, so it is likely that similar mechanisms are used in oilseed rape and Arabidopsis to control H(2)O(2) levels. Under elevated CO(2) conditions, oilseed rape senescence was accelerated and coincided with an earlier increase in H(2)O(2) levels, indicating that H(2)O(2) may be one of the signals to inducing senescence in a broader range of Brassicaceae. © 2012 Institute of Botany, the Chinese Academy of Sciences.

  12. Mapping of QTL for the seed storage proteins cruciferin and napin in a winter oilseed rape doubled haploid population and their inheritance in relation to other seed traits.

    Science.gov (United States)

    Schatzki, Jörg; Ecke, Wolfgang; Becker, Heiko C; Möllers, Christian

    2014-05-01

    Cruciferin (cru) and napin (nap) were negatively correlated and the cru/nap ratio was closely negative correlated with glucosinolate content indicating a link between the two biosynthetic pathways. Canola-type oilseed rape (Brassica napus L.) is an economically important oilseed crop in temperate zones. Apart from the oil, the canola protein shows potential as a value-added food and nutraceutical ingredient. The two major storage protein groups occurring in oilseed rape are the 2 S napins and 12 S cruciferins. The aim of the present study was to analyse the genetic variation and the inheritance of napin and cruciferin content of the seed protein in the winter oilseed rape doubled haploid population Express 617 × R53 and to determine correlations to other seed traits. Seed samples were obtained from field experiments performed in 2 years at two locations with two replicates in Germany. A previously developed molecular marker map of the DH population was used to map quantitative trait loci (QTL) of the relevant traits. The results indicated highly significant effects of the year and the genotype on napin and cruciferin content as well as on the ratio of cruciferin to napin. Heritabilities were comparatively high with 0.79 for napin and 0.77 for cruciferin. Napin and cruciferin showed a significant negative correlation (-0.36**) and a close negative correlation of the cru/nap ratio to glucosinolate content was observed (-0.81**). Three QTL for napin and two QTL for cruciferin were detected, together explaining 47 and 35 % of the phenotypic variance. A major QTL for glucosinolate content was detected on linkage group N19 whose confidence interval overlapped with QTL for napin and cruciferin content. Results indicate a relationship between seed protein composition and glucosinolate content.

  13. Intergeneric hybrids between Brassica napus and Orychophragmus violaceus containing traits of agronomic importance for oilseed rape breeding.

    Science.gov (United States)

    Hu, Q.; Hansen, N.; Laursen, J.; Dixelius, C.; Andersen, B.

    2002-11-01

    Protoplast fusions between Brassica napus and Orychophragmus violaceus for transfer of valuable traits to oilseed rape resulted in 257 somatic hybrid plants. Hybridity was confirmed by morphological, cytological and molecular means. Symmetric fusions gave rise to 131 plants. Fifty eight of these plants had an intermediate morphology and contained nuclear DNA corresponding to the sum of the parental species. All 131 plants were sterile with no pollen grains observed upon flowering. Another 126 plants were derived from asymmetric fusions in which protoplasts of the donor parent O. violaceus were irradiated by 100 or 200-Gy X-rays prior to fusion. Morphologically these plants showed a larger variation compared to the plants regenerated from symmetric fusion experiments. In contrast to plants obtained from symmetric fusions, fertile hybrids were recovered among regenerants from the asymmetric fusions. Twenty four of these plants released viable pollen grains and 14 of the determined 17 plants set seeds after either selfing or backcrossing to B. napus. Fourteen male-sterile plants were identified with female fertility. This observed male sterility most-likely originated from alloplasmic recombination and would be of great potential for the development of a new cytoplasmic male sterility system. The fatty acid composition of the fertile hybrids and their progenies showed a biased distribution towards the B. napus parent, which has a high erucic acid-content type. However, increased levels of palmitic and linoleic acids compared to B. napus were found in subsequent generations, as well as a reduced level of erucic acid.

  14. Divergent patterns of allelic diversity from similar origins: the case of oilseed rape (Brassica napus L.) in China and Australia.

    Science.gov (United States)

    Chen, S; Nelson, M N; Ghamkhar, K; Fu, T; Cowling, W A

    2008-01-01

    Oilseed rape (Brassica napus) in Australia and China have similar origins, with introductions from Europe, Canada, and Japan in the mid 20th century, and there has been some interchange of germplasm between China and Australia since that time. Allelic diversity of 72 B. napus genotypes representing contemporary germplasm in Australia and China, including samples from India, Europe, and Canada, was characterized by 55 polymorphic simple sequence repeat (SSR) markers spanning the entire B. napus genome. Hierarchical clustering and two-dimensional multidimensional scaling identified a Chinese group (China-1) that was separated from "mixed group" of Australian, Chinese (China-2), European, and Canadian lines. A small group from India was distinctly separated from all other B. napus genotypes. Chinese genotypes, especially in the China-1 group, have inherited unique alleles from interspecific crossing, primarily with B. rapa, and the China-2 group has many alleles in common with Australian genotypes. The concept of "private alleles" is introduced to describe both the greater genetic diversity and the genetic distinctiveness of Chinese germplasm, compared with Australian germplasm, after 50 years of breeding from similar origins.

  15. Protocol: optimisation of a grafting protocol for oilseed rape (Brassica napus) for studying long-distance signalling.

    Science.gov (United States)

    Ostendorp, Anna; Pahlow, Steffen; Deke, Jennifer; Thieß, Melanie; Kehr, Julia

    2016-01-01

    Grafting is a well-established technique for studying long-distance transport and signalling processes in higher plants. While oilseed rape has been the subject of comprehensive analyses of xylem and phloem sap to identify macromolecules potentially involved in long-distance information transfer, there is currently no standardised grafting method for this species published. We developed a straightforward collar-free grafting protocol for Brassica napus plants with high reproducibility and success rates. Micrografting of seedlings was done on filter paper. Grafting success on different types of regeneration media was measured short-term after grafting and as the long-term survival rate (>14 days) of grafts after the transfer to hydroponic culture or soil. We compared different methods for grafting B. napus seedlings. Grafting on filter paper with removed cotyledons, a truncated hypocotyl and the addition of low levels of sucrose under long day conditions allowed the highest grafting success. A subsequent long-term hydroponic cultivation of merged grafts showed highest survival rates and best reproducibility.

  16. Hyperspectral and thermal imaging of oilseed rape (Brassica napus) response to fungal species of the genus Alternaria.

    Science.gov (United States)

    Baranowski, Piotr; Jedryczka, Malgorzata; Mazurek, Wojciech; Babula-Skowronska, Danuta; Siedliska, Anna; Kaczmarek, Joanna

    2015-01-01

    In this paper, thermal (8-13 µm) and hyperspectral imaging in visible and near infrared (VNIR) and short wavelength infrared (SWIR) ranges were used to elaborate a method of early detection of biotic stresses caused by fungal species belonging to the genus Alternaria that were host (Alternaria alternata, Alternaria brassicae, and Alternaria brassicicola) and non-host (Alternaria dauci) pathogens to oilseed rape (Brassica napus L.). The measurements of disease severity for chosen dates after inoculation were compared to temperature distributions on infected leaves and to averaged reflectance characteristics. Statistical analysis revealed that leaf temperature distributions on particular days after inoculation and respective spectral characteristics, especially in the SWIR range (1000-2500 nm), significantly differed for the leaves inoculated with A. dauci from the other species of Alternaria as well as from leaves of non-treated plants. The significant differences in leaf temperature of the studied Alternaria species were observed in various stages of infection development. The classification experiments were performed on the hyperspectral data of the leaf surfaces to distinguish days after inoculation and Alternaria species. The second-derivative transformation of the spectral data together with back-propagation neural networks (BNNs) appeared to be the best combination for classification of days after inoculation (prediction accuracy 90.5%) and Alternaria species (prediction accuracy 80.5%).

  17. Genetic variants associated with the root system architecture of oilseed rape (Brassica napus L.) under contrasting phosphate supply.

    Science.gov (United States)

    Wang, Xiaohua; Chen, Yanling; Thomas, Catherine L; Ding, Guangda; Xu, Ping; Shi, Dexu; Grandke, Fabian; Jin, Kemo; Cai, Hongmei; Xu, Fangsen; Yi, Bin; Broadley, Martin R; Shi, Lei

    2017-08-01

    Breeding crops with ideal root system architecture for efficient absorption of phosphorus is an important strategy to reduce the use of phosphate fertilizers. To investigate genetic variants leading to changes in root system architecture, 405 oilseed rape cultivars were genotyped with a 60K Brassica Infinium SNP array in low and high P environments. A total of 285 single-nucleotide polymorphisms were associated with root system architecture traits at varying phosphorus levels. Nine single-nucleotide polymorphisms corroborate a previous linkage analysis of root system architecture quantitative trait loci in the BnaTNDH population. One peak single-nucleotide polymorphism region on A3 was associated with all root system architecture traits and co-localized with a quantitative trait locus for primary root length at low phosphorus. Two more single-nucleotide polymorphism peaks on A5 for root dry weight at low phosphorus were detected in both growth systems and co-localized with a quantitative trait locus for the same trait. The candidate genes identified on A3 form a haplotype 'BnA3Hap', that will be important for understanding the phosphorus/root system interaction and for the incorporation into Brassica napus breeding programs. © The Author 2017. Published by Oxford University Press on behalf of Kazusa DNA Research Institute.

  18. Breeding histories and selection criteria for oilseed rape in Europe and China identified by genome wide pedigree dissection.

    Science.gov (United States)

    Wang, Xiaohua; Long, Yan; Wang, Nian; Zou, Jun; Ding, Guangda; Broadley, Martin R; White, Philip J; Yuan, Pan; Zhang, Qianwen; Luo, Ziliang; Liu, Peifa; Zhao, Hua; Zhang, Ying; Cai, Hongmei; King, Graham J; Xu, Fangsen; Meng, Jinling; Shi, Lei

    2017-05-15

    Selection breeding has played a key role in the improvement of seed yield and quality in oilseed rape (Brassica napus L.). We genotyped Tapidor (European), Ningyou7 (Chinese) and their progenitors with the Brassica 60 K Illumina Infinium SNP array and mapped a total of 29,347 SNP markers onto the reference genome of Darmor-bzh. Identity by descent (IBD) refers to a haplotype segment of a chromosome inherited from a shared common ancestor. IBDs identified on the C subgenome were larger than those on the A subgenome within both the Tapidor and Ningyou7 pedigrees. IBD number and length were greater in the Ningyou7 pedigree than in the Tapidor pedigree. Seventy nine QTLs for flowering time, seed quality and root morphology traits were identified in the IBDs of Tapidor and Ningyou7. Many more candidate genes had been selected within the Ningyou7 pedigree than within the Tapidor pedigree. These results highlight differences in the transfer of favorable gene clusters controlling key traits during selection breeding in Europe and China.

  19. Spillage of Viable Seeds of Oilseed Rape along Transportation Routes: Ecological Risk Assessment and Perspectives on Management Efforts

    Directory of Open Access Journals (Sweden)

    Kathrin Pascher

    2017-09-01

    Full Text Available Seed spillage during handling and transportation promotes establishment and invasion of feral crops into adjacent semi-natural habitats. This is also the case for oilseed rape (OSR, Brassica napus, where seed spillage may lead to establishment of herbicide resistant OSR populations in countries without cultivation of genetically modified OSR. Using data from Austria—where cultivation and import of genetically modified OSR are banned—as a prime example, we demonstrate that ports, oil mills, switchyards, and border railway stations to countries with different electric current systems—where trains have to stop—are the sites of primary concern with respect to seed spillage. Based on the results of the Austrian case study we discuss common measures to limit crop seed spillage which include intensified controls at border railway stations and the mode of seed packing during transportation. We further recommend sufficient cleaning both of goods wagons and of loading areas of trucks and ships as well as an appropriate weed management.

  20. The potential of decision support systems to improve risk assessment for pollen beetle management in winter oilseed rape.

    Science.gov (United States)

    Ferguson, Andrew W; Skellern, Matthew P; Johnen, Andreas; von Richthofen, Julia-Sophie; Watts, Nigel P; Bardsley, Eileen; Murray, Darren A; Cook, Samantha M

    2016-03-01

    The reliance on and extensive use of pyrethroid insecticides have led to pyrethroid resistance in pollen beetle (Meligethes aeneus). Widespread adoption of best practice in pollen beetle management is therefore needed. Decision support systems (DSSs) that identify the risk period(s) for pest migration can help to target monitoring and control efforts, but they must be accurate and labour efficient to gain the support of growers. Weather data and the phenology of pollen beetles in 44 winter oilseed rape crops across England over 4 years were used to compare the performance of two risk management tools: the DSS proPlant expert, which predicts migration risk according to a phenological model and local weather data, and 'rule-based advice', which depends on crop growth stage and a temperature threshold. Both risk management tools were effective in prompting monitoring that would detect breaches of various control thresholds. However, the DSS more accurately predicted migration start and advised significantly fewer days of migration risk, consultation days and monitoring than did rule-based advice. The proPlant expert DSS reliably models pollen beetle phenology. Use of such a DSS can focus monitoring effort to when it is most needed, facilitate the practical use of thresholds and help to prevent unnecessary insecticide applications and the development of insecticide resistance. © 2015 Rothamsted Research Ltd. Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.

  1. Distribution and metabolism of D/L-, L- and D-glufosinate in transgenic, glufosinate-tolerant crops of maize (Zea mays L ssp mays) and oilseed rape (Brassica napus L var napus).

    Science.gov (United States)

    Ruhland, Monika; Engelhardt, Gabriele; Pawlizki, Karlheinz

    2004-07-01

    The aim of the present study was to determine whether post-emergence application of glufosinate to transgenic crops could lead to an increase in residues or to the formation of new, hitherto unknown metabolites. Transgenic oilseed rape and maize plants were treated separately with L-glufosinate, D-glufosinate or the racemic mixture. Whereas about 90% of the applied D-glufosinate was washed off by rain and only 5-6% was metabolised, 13-35% of the applied L-glufosinate remained in the form of metabolites and unchanged herbicide in both transgenic maize and oilseed rape. The main metabolite was N-acetyl-L-glufosinate with total residues of 91% in oilseed rape and 67% in maize, together with small amounts, of 5% in oilseed rape and 28% in maize, of different methylphosphinyl fatty acids. These metabolites were probably formed from L-glufosinate by deamination and subsequent decarboxylation. The residues were distributed in all fractions of the plants, with the highest contents in treated leaves and the lowest in the grains (0.07-0.3% in maize and 0.4-0.6% in oilseed rape). There was no indication of an accumulation of total residues or of residue levels above the official tolerances for glufosinate.

  2. Effect of nitrification inhibitor DMPP on nitrogen leaching, nitrifying organisms, and enzyme activities in a rice-oilseed rape cropping system.

    Science.gov (United States)

    Li, Hua; Liang, Xinqiang; Chen, Yingxu; Lian, Yanfeng; Tian, Guangming; Ni, Wuzhong

    2008-01-01

    DMPP (3,4-dimethylpyrazole phosphate) has been used to reduce nitrogen (N) loss from leaching or denitrification and to improve N supply in agricultural land. However, its impact on soil nitrifying organisms and enzyme activities involved in N cycling is largely unknown. Therefore, an on-farm experiment, for two years, has been conducted, to elucidate the effects of DMPP on mineral N (NH4(+)-N and NO3(-)-N) leaching, nitrifying organisms, and denitrifying enzymes in a rice-oilseed rape cropping system. Three treatments including urea alone (UA), urea + 1% DMPP (DP), and no fertilizer (CK), have been carried out. The results showed that DP enhanced the mean NH4(+)-N concentrations by 19.1%--24.3%, but reduced the mean NO3(-)-N concentrations by 44.9%--56.6% in the leachate, under a two-year rice-rape rotation, compared to the UA treatment. The population of ammonia oxidizing bacteria, the activity of nitrate reductase, and nitrite reductase in the DP treatment decreased about 24.5%--30.9%, 14.9%--43.5%, and 14.7%--31.6%, respectively, as compared to the UA treatment. However, nitrite oxidizing bacteria and hydroxylamine reductase remained almost unaffected by DMPP. It is proposed that DMPP has the potential to either reduce NO3(-)-N leaching by inhibiting ammonia oxidization or N losses from denitrification, which is in favor of the N conversations in the rice-oilseed rape cropping system.

  3. Factors affecting morphogenetic potential in oilseed rape roots of the Skrzeszowicki and Start cultivars

    Directory of Open Access Journals (Sweden)

    Janina Rogozińska

    2014-01-01

    Full Text Available The effect of the origin of root segments, seedling age, growth substances and gelled or liquid media were tested in respect to the morphogenetic potential of rape root segments of Skrzeszowicki (high glucosinolate content and Start (low glucosinolate content cultivars. Callus and roots were formed on all root segments after an approximately 2 week growth period; buds were formed after ca. 4 weeks only on segments adjacent to the hypocotyl. Higher concentrations of auxin and cytokinins were required for bud induction. Cultivar differences in the morphogenetic responses of the root segments were found. They were manifested by the more abundant callus formation (BAP+NAA and more numerous lateral roots and buds (KIN+IBA on segments from the Skrzeszowicki cultivar than from the Start cultivar.

  4. Long-Term Monitoring of Field Trial Sites with Genetically Modified Oilseed Rape (Brassica napus L.) in Saxony-Anhalt, Germany. Fifteen Years Persistence to Date but No Spatial Dispersion.

    Science.gov (United States)

    Belter, Anke

    2016-01-16

    Oilseed rape is known to persist in arable fields because of its ability to develop secondary seed dormancy in certain agronomic and environmental conditions. If conditions change, rapeseeds are able to germinate up to 10 years later to build volunteers in ensuing crops. Extrapolations of experimental data acted on the assumption of persistence periods for more than 20 years after last harvest of rapeseed. Genetically-modified oilseed rape-cultivated widely in Northern America since 1996-is assumed not to differ from its conventional form in this property. Here, experimental data are reported from official monitoring activities that verify these assumptions. At two former field trial sites in Saxony-Anhalt genetically-modified herbicide-resistant oilseed rape volunteers are found up to fifteen years after harvest. Nevertheless, spatial dispersion or establishment of GM plants outside of the field sites was not observed within this period.

  5. Genetic variation and inheritance of phytosterol and oil content in a doubled haploid population derived from the winter oilseed rape Sansibar × Oase cross.

    Science.gov (United States)

    Teh, Lishia; Möllers, Christian

    2016-01-01

    Identification of QTL for phytosterol content, oil content, fatty acids content, protein content of defatted meal, and seed weight by multiple interval mapping in a Brassica napus DH population. Phytosterols are minor seed constituents in oilseed rape which have recently drawn wide-interest from the food and nutrition industry due to their health benefit in lowering LDL cholesterol in humans. To understand the genetic basis of phytosterol content and its relationship with other seed quality traits in oilseed rape, QTL mapping was performed in a segregating DH population derived from the cross of two winter oilseed rape varieties, Sansibar and Oase, termed SODH population. Both parental lines are of canola quality which differ in phytosterol and oil content in seed. A genetic map was constructed for SODH population based on a total of 1638 markers organized in 23 linkage groups and covering a map length of 2350 cM with a mean marker interval of 2.0 cM. The SODH population and the parental lines were cultivated at six environments in Europe and were phenotyped for phytosterol content, oil content, fatty acids content, protein content of the defatted meal, and seed weight. Multiple interval mapping identified between one and six QTL for nine phytosterol traits, between two and six QTL for four fatty acids, five QTL for oil content, four QTL for protein content of defatted meal, and three QTL for seed weight. Colocalizations of QTL for different traits were more frequently observed than individual isolated QTL. Major QTL (R (2) ≥ 25%) were all located in the A genome, and the possible candidate genes were investigated by physical localization of the QTL to the reference genome sequence of Brassica rapa.

  6. The impact of sulfate restriction on seed yield and quality of winter oilseed rape depends on the ability to remobilize sulfate from vegetative tissues to reproductive organs.

    Directory of Open Access Journals (Sweden)

    Alexandra eGirondé

    2014-12-01

    Full Text Available Our current knowledge about sulfur (S management by winter oilseed rape to satisfy the S demand of developing seeds is still scarce, particularly in relation to S restriction. Our goals were to determine the physiological processes related to S use efficiency that led to maintain the seed yield and quality when S limitation occurred at the bolting or early flowering stages. To address these questions, a pulse-chase 34SO42- labelling method was carried out in order to study the S fluxes from uptake and remobilization at the whole plant level. In response of S limitation at the bolting or early flowering stages, the leaves are the most important source organ for S remobilization during reproductive stages. By combining 34S-tracer with biochemical fractionation in order to separate sulfate from other S-compounds, it appeared that sulfate was the main form of S remobilized in leaves at reproductive stages and that tonoplastic SULTR4-type transporters were specifically involved in the sulfate remobilisation in case of low S availability. In response to S limitation at the bolting stage, the seed yield and quality were dramatically reduced compared to control plants. These data suggest that the increase of both S remobilization from source leaves and the root proliferation in order to maximize sulfate uptake capacities, were not sufficient to maintain the seed yield and quality. When S limitation occurred at the early flowering stage, oilseed rape can optimize the mobilization of sulfate reserves from vegetative organs (leaves and stem to satisfy the demand of seeds and maintain the seed yield and quality. Our study also revealed that the stem may act as a transient storage organ for remobilized S coming from source leaves before its utilization by seeds. The physiological traits (S remobilization, root proliferation, transient S storage in stem observed under S limitation could be used in breeding programs to select oilseed rape genotypes with high S

  7. Uptake, transport and distribution of molybdenum in two oilseed rape (Brassica napus L.) cultivars under different nitrate/ammonium ratios.

    Science.gov (United States)

    Qin, Shi-Yu; Sun, Xue-Cheng; Hu, Cheng-Xiao; Tan, Qi-Ling; Zhao, Xiao-Hu

    2017-06-01

    To investigate the effects of different nitrate sources on the uptake, transport, and distribution of molybdenum (Mo) between two oilseed rape (Brassica napus L.) cultivars, L0917 and ZS11. A hydroponic culture experiment was conducted with four nitrate/ammonium (NO3-:NH4+) ratios (14:1, 9:6, 7.5:7.5, and 1:14) at a constant nitrogen concentration of 15 mmol/L. We examined Mo concentrations in roots, shoots, xylem and phloem sap, and subcellular fractions of leaves to contrast Mo uptake, transport, and subcellular distribution between ZS11 and L0917. Both the cultivars showed maximum biomass and Mo accumulation at the 7.5:7.5 ratio of NO3-:NH4+ while those were decreased by the 14:1 and 1:14 treatments. However, the percentages of root Mo (14.8% and 15.0% for L0917 and ZS11, respectively) were low under the 7.5:7.5 treatment, suggesting that the equal NO3-:NH4+ ratio promoted Mo transportation from root to shoot. The xylem sap Mo concentration and phloem sap Mo accumulation of L0917 were lower than those of ZS11 under the 1:14 treatment, which suggests that higher NO3-:NH4+ ratio was more beneficial for L0917. On the contrary, a lower NO3-:NH4+ ratio was more beneficial for ZS11 to transport and remobilize Mo. Furthermore, the Mo concentrations of both the cultivars' leaf organelles were increased but the Mo accumulations of the cell wall and soluble fraction were reduced significantly under the 14:1 treatment, meaning that more Mo was accumulated in organelles under the highest NO3-:NH4+ ratio. This investigation demonstrated that the capacities of Mo absorption, transportation and subcellular distribution play an important role in genotype-dependent differences in Mo accumulation under low or high NO3-:NH4+ ratio conditions.

  8. Genetic Diversity of Oilseed Rape Fields and Feral Populations in the Context of Coexistence with GM Crops.

    Directory of Open Access Journals (Sweden)

    Diane Bailleul

    Full Text Available Despite growing concern about transgenes escaping from fields, few studies have analysed the genetic diversity of crops in an agroecosystem over several years. Accurate information about the dynamics and relationship of the genetic diversity of crops in an agroecosystem is essential for risk assessment and policies concerning the containment of genetically modified crops and their coexistence with crops grown by conventional practices. Here, we analysed the genetic diversity of oilseed rape plants from fields and feral populations over 4 years in an agricultural landscape of 41 km2. We used exact compatibility and maximum likelihood assignment methods to assign these plants to cultivars. Even pure lines and hybrid cultivar seed lots contained several genotypes. The cultivar diversity in fields reflected the conventional view of agroecosystems quite well: that is, there was a succession of cultivars, some grown for longer than others because of their good performance, some used for one year and then abandoned, and others gradually adopted. Three types of field emerged: fields sown with a single cultivar, fields sown with two cultivars, and unassigned fields (too many cultivars or unassigned plants to reliably assign the field. Field plant diversity was higher than expected, indicating the persistence of cultivars that were grown for only one year. The cultivar composition of feral populations was similar to that of field plants, with an increasing number of cultivars each year. By using genetic tools, we found a link between the cultivars of field plants in a particular year and the cultivars of feral population plants in the following year. Feral populations on road verges were more diverse than those on path verges. All of these findings are discussed in terms of their consequences in the context of coexistence with genetically modified crops.

  9. Genetic Diversity of Oilseed Rape Fields and Feral Populations in the Context of Coexistence with GM Crops.

    Science.gov (United States)

    Bailleul, Diane; Ollier, Sébastien; Lecomte, Jane

    2016-01-01

    Despite growing concern about transgenes escaping from fields, few studies have analysed the genetic diversity of crops in an agroecosystem over several years. Accurate information about the dynamics and relationship of the genetic diversity of crops in an agroecosystem is essential for risk assessment and policies concerning the containment of genetically modified crops and their coexistence with crops grown by conventional practices. Here, we analysed the genetic diversity of oilseed rape plants from fields and feral populations over 4 years in an agricultural landscape of 41 km2. We used exact compatibility and maximum likelihood assignment methods to assign these plants to cultivars. Even pure lines and hybrid cultivar seed lots contained several genotypes. The cultivar diversity in fields reflected the conventional view of agroecosystems quite well: that is, there was a succession of cultivars, some grown for longer than others because of their good performance, some used for one year and then abandoned, and others gradually adopted. Three types of field emerged: fields sown with a single cultivar, fields sown with two cultivars, and unassigned fields (too many cultivars or unassigned plants to reliably assign the field). Field plant diversity was higher than expected, indicating the persistence of cultivars that were grown for only one year. The cultivar composition of feral populations was similar to that of field plants, with an increasing number of cultivars each year. By using genetic tools, we found a link between the cultivars of field plants in a particular year and the cultivars of feral population plants in the following year. Feral populations on road verges were more diverse than those on path verges. All of these findings are discussed in terms of their consequences in the context of coexistence with genetically modified crops.

  10. A high-density genetic map identifies a novel major QTL for boron efficiency in oilseed rape (Brassica napus L.).

    Science.gov (United States)

    Zhang, Didi; Hua, Yingpeng; Wang, Xiaohua; Zhao, Hua; Shi, Lei; Xu, Fangsen

    2014-01-01

    Low boron (B) seriously limits the growth of oilseed rape (Brassica napus L.), a high B demand species that is sensitive to low B conditions. Significant genotypic variations in response to B deficiency have been observed among B. napus cultivars. To reveal the genetic basis for B efficiency in B. napus, quantitative trait loci (QTLs) for the plant growth traits, B uptake traits and the B efficiency coefficient (BEC) were analyzed using a doubled haploid (DH) population derived from a cross between a B-efficient parent, Qingyou 10, and a B-inefficient parent, Westar 10. A high-density genetic map was constructed based on single nucleotide polymorphisms (SNPs) assayed using Brassica 60 K Infinium BeadChip Array, simple sequence repeats (SSRs) and amplified fragment length polymorphisms (AFLPs). The linkage map covered a total length of 2139.5 cM, with 19 linkage groups (LGs) and an average distance of 1.6 cM between adjacent markers. Based on hydroponic evaluation of six B efficiency traits measured in three separate repeated trials, a total of 52 QTLs were identified, accounting for 6.14-46.27% of the phenotypic variation. A major QTL for BEC, qBEC-A3a, was co-located on A3 with other QTLs for plant growth and B uptake traits under low B stress. Using a subset of substitution lines, qBEC-A3a was validated and narrowed down to the interval between CNU384 and BnGMS436. The results of this study provide a novel major locus located on A3 for B efficiency in B. napus that will be suitable for fine mapping and marker-assisted selection breeding for B efficiency in B. napus.

  11. A high-density genetic map identifies a novel major QTL for boron efficiency in oilseed rape (Brassica napus L..

    Directory of Open Access Journals (Sweden)

    Didi Zhang

    Full Text Available Low boron (B seriously limits the growth of oilseed rape (Brassica napus L., a high B demand species that is sensitive to low B conditions. Significant genotypic variations in response to B deficiency have been observed among B. napus cultivars. To reveal the genetic basis for B efficiency in B. napus, quantitative trait loci (QTLs for the plant growth traits, B uptake traits and the B efficiency coefficient (BEC were analyzed using a doubled haploid (DH population derived from a cross between a B-efficient parent, Qingyou 10, and a B-inefficient parent, Westar 10. A high-density genetic map was constructed based on single nucleotide polymorphisms (SNPs assayed using Brassica 60 K Infinium BeadChip Array, simple sequence repeats (SSRs and amplified fragment length polymorphisms (AFLPs. The linkage map covered a total length of 2139.5 cM, with 19 linkage groups (LGs and an average distance of 1.6 cM between adjacent markers. Based on hydroponic evaluation of six B efficiency traits measured in three separate repeated trials, a total of 52 QTLs were identified, accounting for 6.14-46.27% of the phenotypic variation. A major QTL for BEC, qBEC-A3a, was co-located on A3 with other QTLs for plant growth and B uptake traits under low B stress. Using a subset of substitution lines, qBEC-A3a was validated and narrowed down to the interval between CNU384 and BnGMS436. The results of this study provide a novel major locus located on A3 for B efficiency in B. napus that will be suitable for fine mapping and marker-assisted selection breeding for B efficiency in B. napus.

  12. Oilseed rape (Brassica napus) as a resource for farmland insect pollinators: quantifying floral traits in conventional varieties and breeding systems.

    Science.gov (United States)

    Carruthers, Jonathan M; Cook, Samantha M; Wright, Geraldine A; Osborne, Juliet L; Clark, Suzanne J; Swain, Jennifer L; Haughton, Alison J

    2017-08-01

    Oilseed rape (OSR; Brassica napus L.) is a major crop in temperate regions and provides an important source of nutrition to many of the yield-enhancing insect flower visitors that consume floral nectar. The manipulation of mechanisms that control various crop plant traits for the benefit of pollinators has been suggested in the bid to increase food security, but little is known about inherent floral trait expression in contemporary OSR varieties or the breeding systems used in OSR breeding programmes. We studied a range of floral traits in glasshouse-grown, certified conventional varieties of winter OSR to test for variation among and within breeding systems. We measured 24-h nectar secretion rate, amount, concentration and ratio of nectar sugars per flower, and sizes and number of flowers produced per plant from 24 varieties of OSR representing open-pollinated (OP), genic male sterility (GMS) hybrid and cytoplasmic male sterility (CMS) hybrid breeding systems. Sugar concentration was consistent among and within the breeding systems; however, GMS hybrids produced more nectar and more sugar per flower than CMS hybrid or OP varieties. With the exception of ratio of fructose/glucose in OP varieties, we found that nectar traits were consistent within all the breeding systems. When scaled, GMS hybrids produced 1.73 times more nectar resource per plant than OP varieties. Nectar production and amount of nectar sugar in OSR plants were independent of number and size of flowers. Our data show that floral traits of glasshouse-grown OSR differed among breeding systems, suggesting that manipulation and enhancement of nectar rewards for insect flower visitors, including pollinators, could be included in future OSR breeding programmes.

  13. Sulphur limitation provokes physiological and leaf proteome changes in oilseed rape that lead to perturbation of sulphur, carbon and oxidative metabolisms

    Science.gov (United States)

    2013-01-01

    Background The decline in industrial emissions of sulphur (S) has led to a sulphate depletion in soil resulting in an alteration of crop performance. In oilseed rape, an S deficiency dramatically reduced the seed yield and/or quality. Paradoxically, little is known about the impact of sulphate limitation on oilseed rape leaf metabolism, despite it being a key determinant of growth. In order to identify the metabolic processes involved in the oilseed rape response to S restriction, an analysis of the young leaf proteome combined with a physiological study was carried out at the vegetative stage. Results S limitation does not significantly reduce the total shoot biomass but inhibits growth and photosynthesis of young leaves. This photosynthesis decline is not due to a decrease in chlorophyll content, which remains similar to Control. The increase in anthocyanins and H2O2 content in young leaves of S-limited plants suggests that S restriction leads to an oxidative stress. Proteomic analysis at 35 d of S limitation also revealed the induction of 12-oxophitodienoate reductase and ACC synthase, respectively involved in jasmonate and ethylene biosynthesis, two phytohormones that could be implicated in oxidative stress. Proteins involved in photosynthesis and carbon metabolism were also modulated by S restriction. In particular, the decrease in plastocyanin and ferredoxin–NADP reductase suggests that H2O2 accumulation is associated with perturbation of the photosynthetic electron transport chain. The accumulation of chloroplastic Cu-Zn SOD reinforces the idea that an oxidative stress probably occurs in the chloroplast. Proteomic results suggest that the maintenance of chlorophyll in S-limited conditions is related to an accumulation of Water Soluble Chlorophyll binding Proteins, involved in the protection of chlorophyll against ROS. The accumulation of the catalytic α–subunit of chloroplastic ATP synthase suggests that energy production is maintained. Conclusion S

  14. Impact of different soil cultivation on weed species in winter rape (oilseed

    Directory of Open Access Journals (Sweden)

    Jan Winkler

    2005-01-01

    Full Text Available A community of weeds and crops is affected by a number of factors, including, among other things, also tillage. In the years 2000–2002, the composition of weed species in rape stands was evaluated on the fields with the total area of 551 hectares (1 hectar equals to some 2.47 acres. The evaluation was carried out with the application of methodology developed by Kühn (1982. On the fields located in the cadastral area of Olomouc – Holice, which had been cultivated in a traditional manner, 115 relevés were recorded. On the fields in the cadastral area of Bohuňovice, which had been cultivated with the application of reduced tillage, 97 relevés were recorded and evaluated. All of the above fields were subjected to the application of chemical agents reducing the occurrence of weeds. The data thus received were processed by means of multidimensional analysis of ecological data with the application of a RDA method (Redundancy Analysis. In the course of three years, 75 weed species were found on the fields under conventional tillage, on the average, 8.2 species per a relevé, while 66 weed species were found during the same period of time on the fields cultivated by means of reduced tillage, on the average, 8.6 species per a relevé. The application of RDA analysis enabled us to sort out the selected species of weeds (i.e. those the frequency of occurrence of which exceeded 15 % into three groups. The conditions provided by the conventional tillage appeared to be more satisfactory for the weed species included in the first group (Arctium tomentosum, Elytrigia repens, Helianthus tuberosus, Chenopodium album, Lolium perenne and Papaver rhoeas. The species included in the second group, i.e. Alsinula media, Apera spica-venti, Atriplex patula, Bromus sterilis, Capsella bursa-pastoris, Equisetum arvense, Fallopia convolvulus, Myosotis arvensis and Thlaspi arvense., responded to reduced tillage by the increase in cover or by increased frequency of

  15. Enhanced Cd extraction of oilseed rape (Brassica napus) by plant growth-promoting bacteria isolated from Cd hyperaccumulator Sedum alfredii Hance.

    Science.gov (United States)

    Pan, Fengshan; Meng, Qian; Luo, Sha; Shen, Jing; Chen, Bao; Khan, Kiran Yasmin; Japenga, Jan; Ma, Xiaoxiao; Yang, Xiaoe; Feng, Ying

    2017-03-04

    Four plant growth-promoting bacteria (PGPB) were used as study materials, among them two heavy metal-tolerant rhizosphere strains SrN1 (Arthrobacter sp.) and SrN9 (Bacillus altitudinis) were isolated from rhizosphere soil, while two endophytic strains SaN1 (Bacillus megaterium) and SaMR12 (Sphingomonas) were identified from roots of the cadmium (Cd)/zinc (Zn) hyperaccumulator Sedum alfredii Hance. A pot experiment was carried out to investigate the effects of these PGPB on plant growth and Cd accumulation of oilseed rape (Brassica napus) plants grown on aged Cd-spiked soil. The results showed that the four PGPB significantly boosted oilseed rape shoot biomass production, improved soil and plant analyzer development (SPAD) value, enhanced Cd uptake of plant and Cd translocation to the leaves. By fluorescent in situ hybridization (FISH) and green fluorescent protein (GFP), we demonstrated the studied S. alfredii endophytic bacterium SaMR12 were able to colonize successfully in the B. napus roots. However, all four PGPB could increase seed Cd accumulation. Due to its potential to enhance Cd uptake by the plant and to restrict Cd accumulation in the seeds, SaMR12 was selected as the most promising microbial partner of B. napus when setting up a plant-microbe fortified remediation system.

  16. Nano-TiO2 Is Not Phytotoxic As Revealed by the Oilseed Rape Growth and Photosynthetic Apparatus Ultra-Structural Response.

    Directory of Open Access Journals (Sweden)

    Jun Li

    Full Text Available Recently nano-materials are widely used but they have shown contrasting effects on human and plant life. Keeping in view the contrasting results, the present study has evaluated plant growth response, antioxidant system activity and photosynthetic apparatus physiological and ultrastructural changes in Brassica napus L. plants grown under a wide range (0, 500, 2500, 4000 mg/l of nano-TiO2 in a pot experiment. Nano-TiO2 has significantly improved the morphological and physiological indices of oilseed rape plants under our experimental conditions. All the parameters i-e morphological (root length, plant height, fresh biomass, physiological (photosynthetic gas exchange, chlorophyll content, nitrate reductase activity and antioxidant system (Superoxide dismutase, SOD; Guaiacol peroxidase, POD; Catalase, CAT recorded have shown improvement in their performance by following nano-TiO2 dose-dependent manner. No significant chloroplast ultra-structural changes were observed. Transmission electron microscopic images have shown that intact & typical grana and stroma thylakoid membranes were in the chloroplast, which suggest that nano-TiO2 has not induced the stressful environment within chloroplast. Finally, it is suggested that, nano-TiO2 have growth promoting effect on oilseed rape plants.

  17. [Effect of atmospheric CO2 concentration and nitrogen application level on absorption and transportation of nutrient elements in oilseed rape].

    Science.gov (United States)

    Wang, Wen-ming; Zhang, Zhen-hua; Song, Hai-xing; Liu, Qiang; Rong, Xiang-min; Guan, Chun-yun; Zeng, Jing; Yuan, Dan

    2015-07-01

    Effect of elevated atmospheric-CO2 (780 µmol . mol-1) on the absorption and transportation of secondary nutrient elements (calcium, magnesium, sulphur) and micronutrient elements (iron, manganese, zinc, molybdenum and boron) in oilseed rape at the stem elongation stage were studied by greenhouse simulated method. Compared with the ambient CO2 condition, the content of Zn in stem was increased and the contents of other nutrient elements were decreased under the elevated atmospheric-CO2 with no nitrogen (N) application; the contents of Ca, S, B and Zn were increased, and the contents of Mg, Mn, Mo and Fe were decreased under the elevated atmospheric CO2 with N application (0.2 g N . kg-1 soil); except the content of Mo in leaf was increased, the contents of other nutrient elements were decreased under the elevated atmospheric-CO2 with two levels of N application. Compared with the ambient CO2 condition, the amounts of Ca and S relative to the total amount of secondary nutrient elements in stem and the amounts of B and Zn relative to the total amount of micronutrient elements in stem were increased under the elevated-CO2 treatment with both levels of N application, and the corresponding values of Mg, Fe, Mn and Mo were decreased; no-N application treatment increased the proportion of Ca distributed into the leaves, and the proportion of Mg distributed into leaves was increased by the normal-N application level; the proportions of Mn, Zn and Mo distributed into the leaves were increased at both N application levels. Without N application, the elevation of atmospheric CO2 increased the transport coefficients of SFe, Mo and SS,B, but decreased the transport coefficients of SMg,Fe, SMg, Mn and SS,Fe, indicating the proportions of Mo, S transported into the upper part of plant tissues was higher than that of Fe, and the corresponding value of B was higher than that observed for S, the corresponding value of Mg was higher than that of Fe and Mn. Under normal-N application

  18. Identification and characterization of microRNAs in oilseed rape (Brassica napus) responsive to infection with the pathogenic fungus Verticillium longisporum using Brassica AA (Brassica rapa) and CC (Brassica oleracea) as reference genomes

    National Research Council Canada - National Science Library

    Shen, Dan; Suhrkamp, Ina; Wang, Yu; Liu, Shenyi; Menkhaus, Jan; Verreet, Joseph‐Alexander; Fan, Longjiang; Cai, Daguang

    2014-01-01

    .... To identify oilseed rape mi RNA s, we deep‐sequenced two small RNA libraries made from V. longisporum infected/noninfected roots and employed Brassica rapa and Brassica oleracea genomes as references for mi RNA prediction and characterization...

  19. Overexpression of Sinapine Esterase BnSCE3 in Oilseed Rape Seeds Triggers Global Changes in Seed Metabolism1[W][OA

    Science.gov (United States)

    Clauß, Kathleen; von Roepenack-Lahaye, Edda; Böttcher, Christoph; Roth, Mary R.; Welti, Ruth; Erban, Alexander; Kopka, Joachim; Scheel, Dierk; Milkowski, Carsten; Strack, Dieter

    2011-01-01

    Sinapine (O-sinapoylcholine) is the predominant phenolic compound in a complex group of sinapate esters in seeds of oilseed rape (Brassica napus). Sinapine has antinutritive activity and prevents the use of seed protein for food and feed. A strategy was developed to lower its content in seeds by expressing an enzyme that hydrolyzes sinapine in developing rape seeds. During early stages of seedling development, a sinapine esterase (BnSCE3) hydrolyzes sinapine, releasing choline and sinapate. A portion of choline enters the phospholipid metabolism, and sinapate is routed via 1-O-sinapoyl-β-glucose into sinapoylmalate. Transgenic oilseed rape lines were generated expressing BnSCE3 under the control of a seed-specific promoter. Two distinct single-copy transgene insertion lines were isolated and propagated to generate homozygous lines, which were subjected to comprehensive phenotyping. Sinapine levels of transgenic seeds were less than 5% of wild-type levels, whereas choline levels were increased. Weight, size, and water content of transgenic seeds were significantly higher than those of wild-type seeds. Seed quality parameters, such as fiber and glucosinolate levels, and agronomically important traits, such as oil and protein contents, differed only slightly, except that amounts of hemicellulose and cellulose were about 30% higher in transgenic compared with wild-type seeds. Electron microscopic examination revealed that a fraction of the transgenic seeds had morphological alterations, characterized by large cavities near the embryonic tissue. Transgenic seedlings were larger than wild-type seedlings, and young seedlings exhibited longer hypocotyls. Examination of metabolic profiles of transgenic seeds indicated that besides suppression of sinapine accumulation, there were other dramatic differences in primary and secondary metabolism. Mapping of these changes onto metabolic pathways revealed global effects of the transgenic BnSCE3 expression on seed metabolism. PMID

  20. Loop-mediated isothermal amplification as a good tool to study changing Leptosphaeria populations in oilseed rape plants and air samples

    Directory of Open Access Journals (Sweden)

    Małgorzata Jędryczka

    2014-01-01

    Full Text Available LAMP is an innovative, simple, rapid, specific and cost-effective nucleic acid amplification method. Due to the use of a special enzyme – GspSSD polymerase, the reaction takes a short time and can be performed at isothermal conditions. The sensitivity and specificity of LAMP technique is significantly higher, than standard PCR techniques, as two or three specific primer pairs are used. The technique is regarded as a useful tool for the detection and identification of plant pathogens. In this work, LAMP was used to study the composition of the population of fungi of the genus Leptosphaeria, causing a damaging disease of oilseed rape, called blackleg or stem canker. The detection concerned DNA present in fungal spores contained in air samples obtained using Hirst-type volumetric trap, in Pomerania (north Poland in 2010. The results achieved using the LAMP technique were similar to these obtained with previously used, highly specific method of Real-time PCR. Conducting LAMP reaction was much easier and less time-and cost-consuming, due to a simplified method of DNA isolation of pathogens from plant tissues. Then, the LAMP technique was used to assess the composition of the population of Leptosphaeria spp. in plants of oil- seed rape collected from the field in the Opole region (south-we- stern part of Poland in 2013. In contrast to studies conducted in 2002–2003, the analysis of leaf symptoms showed a higher pro- portion of L. maculans compared to L. biglobosa, what reflects changes in the composition of pathogen population of fungi causing blackleg on oilseed rape in this part of Poland.

  1. Long-Term Monitoring of Field Trial Sites with Genetically Modified Oilseed Rape (Brassica napus L.) in Saxony-Anhalt, Germany. Fifteen Years Persistence to Date but No Spatial Dispersion

    Science.gov (United States)

    Belter, Anke

    2016-01-01

    Oilseed rape is known to persist in arable fields because of its ability to develop secondary seed dormancy in certain agronomic and environmental conditions. If conditions change, rapeseeds are able to germinate up to 10 years later to build volunteers in ensuing crops. Extrapolations of experimental data acted on the assumption of persistence periods for more than 20 years after last harvest of rapeseed. Genetically-modified oilseed rape—cultivated widely in Northern America since 1996—is assumed not to differ from its conventional form in this property. Here, experimental data are reported from official monitoring activities that verify these assumptions. At two former field trial sites in Saxony-Anhalt genetically-modified herbicide-resistant oilseed rape volunteers are found up to fifteen years after harvest. Nevertheless, spatial dispersion or establishment of GM plants outside of the field sites was not observed within this period. PMID:26784233

  2. Frequency-dependent fitness of hybrids between oilseed rape (¤Brassica napus¤) and weedy ¤B. rapa¤ (Brassicaceae)

    DEFF Research Database (Denmark)

    Hauser, T.P.; Damgaard, C.; Bagger Jørgensen, Rikke

    2003-01-01

    Fitness of interspecific hybrids is sometimes high relative to their parents, despite the conventional belief that they are mostly unfit. F-1 hybrids between oilseed rape (Brassica napus) and weedy B. rapa can be significantly more fit than their weedy parents under some conditions; however, under...... other conditions they are less fit. To understand the reasons, we measured the seed production of B. napus, B. rapa, and different generations of hybrid plants at three different densities and in mixtures of different frequencies (including pure stands). Brassica napus, B. rapa, and backcross plants (F...... and reproductive interactions may be responsible for these effects. Our results show that the fitness of both parents and hybrids is strongly frequency-dependent and that the likelihood of introgression of genes between the species thus may depend on the numbers and densities of parents and their various hybrid...

  3. Composting of rice straw with oilseed rape cake and poultry manure and its effects on faba bean (Vicia faba L.) growth and soil properties.

    Science.gov (United States)

    Abdelhamid, Magdi T; Horiuchi, Takatsugu; Oba, Shinya

    2004-06-01

    Composting of rice straw with poultry manure and oilseed rape cake and its effects on growth and yield of faba bean and soil properties was studied in pot experiments at Gifu University, Japan in 2001/2002. The composts reached maturity in 90 days, were rich in organic matter and mineral nutrients, had a high level of stability, and no phytotoxicity. The addition of compost (20-200 g pot(-1)) improved selected soil chemical (increased total N, total C and CEC), physical (decreased particle density) and biological (increased soil respiration rate) properties. Application of composts at a rate of 20 g pot(-1) significantly increased growth, yield, yield components and total crude protein of faba bean plants. The benefit of this compost without chemical fertilizer demonstrated the validity and possibility of sustainable agronomic performance of faba bean using locally available recycled organic materials. Copyright 2003 Elsevier Ltd.

  4. Colocalization of a partially dominant gene for yellow seed colour with a major QTL influencing acid detergent fibre (ADF) content in different crosses of oilseed rape (Brassica napus).

    Science.gov (United States)

    Badani, Ana Gloria; Snowdon, Rod J; Wittkop, Benjamin; Lipsa, Florin D; Baetzel, Roland; Horn, Renate; De Haro, Antonio; Font, Rafael; Lühs, Wilfred; Friedt, Wolfgang

    2006-12-01

    Quantitative trait loci (QTLs) contributing to yellow seed colour and acid detergent fibre (ADF) were localized and compared in 3 mapping populations developed from 2 crosses (designated 'YE1' and 'YE2') between 2 distinct sources of true-breeding yellow-seeded oilseed rape (Brassica napus) and 2 different black-seeded genotypes. A clear correlation was observed between seed colour and ADF content in both crosses. In all 3 populations, a major QTL, with a large effect on both seed colour and ADF in multiple environments, was detected at the same position on chromosome N18. In YE1, a second minor QTL, with a small effect on seed colour but not on ADF content, was localized on chromosome N1. In YE2, no QTL was observed on N1; however, 2 minor seed-colour loci were localized to N15 and N5. A second major QTL for ADF was localized in YE1 on N13; in YE2, no other QTLs for ADF were detected. Combined QTL and segregation data for seed colour and ADF content in the different populations suggest that a partially dominant B. napus gene for seed colour on N18 contributes to a reduction in fibre content in different yellow-seeded B. napus genotypes. The other QTLs that were identified appear to represent different genes in the 2 yellow-seeded rapeseed sources, which, in each case, affect only fibre content or seed colour, respectively. Potential candidate genes and implications for marker-assisted breeding of oilseed rape with reduced seed dietary fibre content are discussed.

  5. Impact of transgenic oilseed rape expressing oryzacystatin-1 (OC-1) and of insecticidal proteins on longevity and digestive enzymes of the solitary bee Osmia bicornis.

    Science.gov (United States)

    Konrad, Roger; Connor, Melanie; Ferry, Natalie; Gatehouse, Angharad M R; Babendreier, Dirk

    2009-04-01

    The risk that insect-resistant transgenic plants may pose for solitary bees was assessed by determining longevity of adult Osmia bicornis (O. rufa) chronically exposed to transgenic oilseed rape expressing oryzacystatin-1 (OC-1) or to the purified insecticidal proteins recombinant rOC-1, Kunitz soybean trypsin inhibitor (SBTI), Galanthus nivalis agglutinin (GNA), or Bacillus thuringiensis toxin Cry1Ab dissolved in sugar solution (at 0.01 and 0.1%, w:v, Cry1Ab only at 0.01%). Compared to control bees, longevity was significantly reduced by SBTI and GNA at both concentrations and by rOC-1 at 0.1%, but not by Cry1Ab or rOC-1 at 0.01%. Longevity on the OC-1 oilseed rape was not significantly different from the control plants. The effects of SBTI and rOC-1 on longevity were investigated through characterization of the digestive proteinases of O. bicornis and analysis of the response in proteinase profiles to ingestion of these proteinase inhibitors. A relatively complex profile of at least four types of soluble proteolytic enzymes was identified. Serine proteinases were found to be predominant, with metallo and especially cysteine proteinases making a smaller albeit significant contribution. The compensatory response to in vivo enzyme inhibition was similar for SBTI and rOC-1 although less pronounced for rOC-1. It consisted of a non-specific overproduction of native proteinases, both sensitive and insensitive, and the induction of a novel aspartic proteinase.

  6. Potential effects of oilseed rape expressing oryzacystatin-1 (OC-1 and of purified insecticidal proteins on larvae of the solitary bee Osmia bicornis.

    Directory of Open Access Journals (Sweden)

    Roger Konrad

    Full Text Available Despite their importance as pollinators in crops and wild plants, solitary bees have not previously been included in non-target testing of insect-resistant transgenic crop plants. Larvae of many solitary bees feed almost exclusively on pollen and thus could be highly exposed to transgene products expressed in the pollen. The potential effects of pollen from oilseed rape expressing the cysteine protease inhibitor oryzacystatin-1 (OC-1 were investigated on larvae of the solitary bee Osmia bicornis (= O. rufa. Furthermore, recombinant OC-1 (rOC-1, the Bt toxin Cry1Ab and the snowdrop lectin Galanthus nivalis agglutinin (GNA were evaluated for effects on the life history parameters of this important pollinator. Pollen provisions from transgenic OC-1 oilseed rape did not affect overall development. Similarly, high doses of rOC-1 and Cry1Ab as well as a low dose of GNA failed to cause any significant effects. However, a high dose of GNA (0.1% in the larval diet resulted in significantly increased development time and reduced efficiency in conversion of pollen food into larval body weight. Our results suggest that OC-1 and Cry1Ab expressing transgenic crops would pose a negligible risk for O. bicornis larvae, whereas GNA expressing plants could cause detrimental effects, but only if bees were exposed to high levels of the protein. The described bioassay with bee brood is not only suitable for early tier non-target tests of transgenic plants, but also has broader applicability to other crop protection products.

  7. Contribution of Nitrogen Uptake and Retranslocation during Reproductive Growth to the Nitrogen Efficiency of Winter Oilseed-Rape Cultivars (Brassica napus L. Differing in Leaf Senescence

    Directory of Open Access Journals (Sweden)

    Fabian Koeslin-Findeklee

    2016-01-01

    Full Text Available Genotypic variation in N efficiency defined as high grain yield under limited nitrogen (N supply of winter oilseed-rape line-cultivars has been predominantly attributed to N uptake efficiency (NUPT through maintained N uptake during reproductive growth related to functional stay-green. For investigating the role of stay-green, N retranslocation and N uptake during the reproductive phase for grain yield formation, two line cultivars differing in N starvation-induced leaf senescence were grown in a field experiment without mineral N (N0 and with 160 kg N·ha−1 (N160. Through frequent harvests from full flowering until maturity N uptake, N utilization and apparent N remobilization from vegetative plant parts to the pods could be calculated. NUPT proved being more important than N utilization efficiency (NUE for grain yield formation under N-limiting (N0 conditions. For cultivar differences in N efficiency, particularly N uptake during flowering (NUPT and biomass allocation efficiency (HI to the grains, were decisive. Both crop traits were related to delayed senescence of the older leaves. Remobilization of N particularly from stems and leaves was more important for pod N accumulation than N uptake after full flowering. Pod walls (high N concentrations and stems (high biomass mainly contributed to the crop-residue N at maturity. Decreasing the crop-inherent high N budget surplus of winter oilseed-rape requires increasing the low N remobilization efficiency particularly of pod-wall N to the grains. Addressing this conclusion, multi-year and -location field experiments with an extended range of cultivars including hybrids are desirable.

  8. Control of slug damage to oilseed rape and wheat with imidacloprid seed dressings in laboratory and field experiments

    NARCIS (Netherlands)

    Simms, L.C.; Ester, A.; Wilson, M.J.

    2006-01-01

    Slugs are common pests of oilseed and cereal crops in Europe and are currently controlled using bait pellets that often fail to give adequate protection: Here we investigate the potential of the broad-spectrum insecticide imidacloprid, previously suggested to have activity against slugs, to control

  9. Study of Drought and Plant Growth Promoting Rhizobacteria (PGPR on Radiation Use Efficiency and Dry Matter Partitioning Into Pod in Different Cultivars of Oilseed Rape (Brassica napus L.

    Directory of Open Access Journals (Sweden)

    Pooya Arvin

    2016-07-01

    Full Text Available Introduction Oilseed rape (Brassica napus L. is one of the valuable oilseed crops which has been attracting attention in recent years (Arvin et al., 2011. Several factors such as water shortage, low relative humidity, heat and salinity can make drought stress. Radiation use efficiency and yield components are the effective factors of yield formation in oilseed rape. Drought tolerance in oilseed rape depends on other factors except radiation use efficiency. Studies have indicated that plant growth promoting rhizobacterias (PGPRs has a direct effect on growth and they can cause resistance to the abiotic stress as well. Hence, considering the drought climate in Iran and the effects of PGPRs on increasing resistance to abiotic stress and relief of drought effects and the importance of the cultivation of oilseed rape in Iran, the present research was done with the goal of study of drought and some plant growth promoting rhizobacteria (PGPR on radiation use efficiency and dry matter partitioning into pod in different cultivars of oilseed rape. Materials and methods The current study was done on the basis of two simultaneous experiments (under stress and non-stress experiments during 2010- 2011 growing season at Agriculture and Natural Resources Research Station of Torogh, Mashhad is in East-North of Iran (36° N, 59° E, 1003 as.Two research sites (under stress and non-stress fields were beside each other. This region has a semi-arid climate (annual rainfall 286 mm. The experimental design was factorial based on randomized compeletly block design with three replications in each experiment. The first treatment was plant growth promoting rizobactria, including B0: no inoculation (control, B1:co-inoculation (Pseudomonas flourescens 169+P. putida 108, B2: inoculation with P. flourescens 169 and B3: inoculation with P. putida 108. Second treatment was cultivar, including Hayola401 and Hayola330 cultivars belong to Brassica napus, Parkland and Goldrush

  10. Proteomic Investigations of Proteases Involved in Cotyledon Senescence: A Model to Explore the Genotypic Variability of Proteolysis Machinery Associated with Nitrogen Remobilization Efficiency during the Leaf Senescence of Oilseed Rape

    OpenAIRE

    Marine Poret; Balakumaran Chandrasekar; van der Hoorn, Renier A. L.; Laurent Coquet; Thierry Jouenne; Jean-Christophe Avice

    2017-01-01

    Oilseed rape is characterized by a low nitrogen remobilization efficiency during leaf senescence, mainly due to a lack of proteolysis. Because cotyledons are subjected to senescence, it was hypothesized that contrasting protease activities between genotypes may be distinguishable early in the senescence of cotyledons. To verify this assumption, our goals were to (i) characterize protease activities in cotyledons between two genotypes with contrasting nitrogen remobilization efficiency (Ténor ...

  11. Processing-bioprocessing of oilseed rape in bioenergy production and value added utilization of remaining seed components

    OpenAIRE

    Bagger, Christian; Bellostas, Natalia; Jensen, Søren K.; Sørensen, Jens C.; Sørensen, Hilmer; Sørensen, Susanne

    2007-01-01

    Cruciferous oilseed crops accumulate relatively high concentrations of oil, proteins and dietary fibres (DF) in their seeds, in addition to bioactive components as glucosinolates and myrosinase isoenzymes (thioglucohydrolase; EC 3.2.1.147). When mixed in the presence of moisture, myrosinase isoenzymes and associated components transform glucosinolates into various types of products, which reduces the value of the extracted oil and the remaining seed components, as well as producing unwanted e...

  12. Alleviation of lead toxicity by 5-aminolevulinic acid is related to elevated growth, photosynthesis, and suppressed ultrastructural damages in oilseed rape.

    Science.gov (United States)

    Tian, Tian; Ali, Basharat; Qin, Yebo; Malik, Zaffar; Gill, Rafaqat A; Ali, Shafaqat; Zhou, Weijun

    2014-01-01

    Lead (Pb) is a widely spread pollutant and leads to diverse morphological and structural changes in the plants. In this study, alleviating role of 5-aminolevulinic acid (ALA) in oilseed rape (Brassica napus L.) was investigated with or without foliar application of ALA (25 mg L(-1)) in hydroponic environment under different Pb levels (0, 100, and 400 µM). Outcomes stated that plant morphology and photosynthetic attributes were reduced under the application of Pb alone. However, ALA application significantly increased the plant growth and photosynthetic parameters under Pb toxicity. Moreover, ALA also lowered the Pb concentration in shoots and roots under Pb toxicity. The microscopic studies depicted that exogenously applied ALA ameliorated the Pb stress and significantly improved the cell ultrastructures. After application of ALA under Pb stress, mesophyll cell had well-developed nucleus and chloroplast having a number of starch granules. Moreover, micrographs illustrated that root tip cell contained well-developed nucleus, a number of mitochondria, and golgi bodies. These results proposed that under 15-day Pb-induced stress, ALA improved the plant growth, chlorophyll content, photosynthetic parameters, and ultrastructural modifications in leaf mesophyll and root tip cells of the B. napus plants.

  13. CRISPR-Cas9 Targeted Mutagenesis Leads to Simultaneous Modification of Different Homoeologous Gene Copies in Polyploid Oilseed Rape (Brassica napus).

    Science.gov (United States)

    Braatz, Janina; Harloff, Hans-Joachim; Mascher, Martin; Stein, Nils; Himmelbach, Axel; Jung, Christian

    2017-06-01

    In polyploid species, altering a trait by random mutagenesis is highly inefficient due to gene redundancy. We have stably transformed tetraploid oilseed rape (Brassica napus) with a CRISPR-Cas9 construct targeting two ALCATRAZ (ALC) homoeologs. ALC is involved in valve margin development and, thus, contributes to seed shattering from mature fruits. Knocking out ALC would increase shatter resistance to avoid seed loss during mechanical harvest. We obtained a transgenic T1 plant with four alc mutant alleles by the use of a single target sequence. All mutations were stably inherited to the T2 progeny. The T2 generation was devoid of any wild-type alleles, proving that the underlying T1 was a nonchimeric double heterozygote. T-DNA and ALC loci were not linked, as indicated by random segregation in the T2 generation. Hence, we could select double mutants lacking the T-DNA already in the first offspring generation. However, whole-genome sequencing data revealed at least five independent insertions of vector backbone sequences. We did not detect any off-target effects in two genome regions homologous to the target sequence. The simultaneous alteration of multiple homoeologs by CRISPR-Cas9 mutagenesis without any background mutations will offer new opportunities for using mutant genotypes in rapeseed breeding. © 2017 American Society of Plant Biologists. All Rights Reserved.

  14. A Comparative Study of Proteolytic Mechanisms during Leaf Senescence of Four Genotypes of Winter Oilseed Rape Highlighted Relevant Physiological and Molecular Traits for NRE Improvement

    Directory of Open Access Journals (Sweden)

    Alexandra Girondé

    2015-12-01

    Full Text Available Winter oilseed rape is characterized by a low N use efficiency related to a weak leaf N remobilization efficiency (NRE at vegetative stages. By investigating the natural genotypic variability of leaf NRE, our goal was to characterize the relevant physiological traits and the main protease classes associated with an efficient proteolysis and high leaf NRE in response to ample or restricted nitrate supply. The degradation rate of soluble proteins and D1 protein (a thylakoid-bound protein were correlated to N remobilization, except for the genotype Samouraï which showed a low NRE despite high levels of proteolysis. Under restricted nitrate conditions, high levels of soluble protein degradation were associated with serine, cysteine and aspartic proteases at acidic pH. Low leaf NRE was related to a weak proteolysis of both soluble and thylakoid-bound proteins. The results obtained on the genotype Samouraï suggest that the timing between the onset of proteolysis and abscission could be a determinant. The specific involvement of acidic proteases suggests that autophagy and/or senescence-associated vacuoles are implicated in N remobilization under low N conditions. The data revealed that the rate of D1 degradation could be a relevant indicator of leaf NRE and might be used as a tool for plant breeding.

  15. Improved biogas production and biodegradation of oilseed rape straw by using kitchen waste and duck droppings as co-substrates in two-phase anaerobic digestion

    Science.gov (United States)

    Wang, Chuqiao; Hong, Feng; Lu, Yong; Liu, Hengming

    2017-01-01

    Oilseed rape straw (ORS) is a kind of biorefractory waste widely existing in the rural area of China, which is highly suitable to mix with kitchen waste (KW) and duck droppings (DD) in two-phase anaerobic digestion (AD). This research introduced the importance of KW and DD addition to improve the biogas production and biodegradation of ORS. A set of comparative experiments were conducted on two-phase mono- and co-digestion with organic load of 60 g VS/L. The total methane yield (TMY) and the biodegradation of ORS of co-digestions were obviously improving, and the synergistic effect found in the two-phase co-digestions. The optimum mixing ratio of ORS, KW and DD was 50:40:10, and the corresponding TMY and VS degradation rate of ORS were 374.5 mL/g VS and 49.7%, respectively. Addition of KW and DD maintained the pH within the optimal range for the hydrolyzing-acidification, improved the phase separation and buffering capacity of AD system. PMID:28767709

  16. Alleviation of Lead Toxicity by 5-Aminolevulinic Acid Is Related to Elevated Growth, Photosynthesis, and Suppressed Ultrastructural Damages in Oilseed Rape

    Directory of Open Access Journals (Sweden)

    Tian Tian

    2014-01-01

    Full Text Available Lead (Pb is a widely spread pollutant and leads to diverse morphological and structural changes in the plants. In this study, alleviating role of 5-aminolevulinic acid (ALA in oilseed rape (Brassica napus L. was investigated with or without foliar application of ALA (25 mg L−1 in hydroponic environment under different Pb levels (0, 100, and 400 µM. Outcomes stated that plant morphology and photosynthetic attributes were reduced under the application of Pb alone. However, ALA application significantly increased the plant growth and photosynthetic parameters under Pb toxicity. Moreover, ALA also lowered the Pb concentration in shoots and roots under Pb toxicity. The microscopic studies depicted that exogenously applied ALA ameliorated the Pb stress and significantly improved the cell ultrastructures. After application of ALA under Pb stress, mesophyll cell had well-developed nucleus and chloroplast having a number of starch granules. Moreover, micrographs illustrated that root tip cell contained well-developed nucleus, a number of mitochondria, and golgi bodies. These results proposed that under 15-day Pb-induced stress, ALA improved the plant growth, chlorophyll content, photosynthetic parameters, and ultrastructural modifications in leaf mesophyll and root tip cells of the B. napus plants.

  17. A Comparative Study of Proteolytic Mechanisms during Leaf Senescence of Four Genotypes of Winter Oilseed Rape Highlighted Relevant Physiological and Molecular Traits for NRE Improvement

    Science.gov (United States)

    Girondé, Alexandra; Poret, Marine; Etienne, Philippe; Trouverie, Jacques; Bouchereau, Alain; Le Cahérec, Françoise; Leport, Laurent; Niogret, Marie-Françoise; Avice, Jean-Christophe

    2015-01-01

    Winter oilseed rape is characterized by a low N use efficiency related to a weak leaf N remobilization efficiency (NRE) at vegetative stages. By investigating the natural genotypic variability of leaf NRE, our goal was to characterize the relevant physiological traits and the main protease classes associated with an efficient proteolysis and high leaf NRE in response to ample or restricted nitrate supply. The degradation rate of soluble proteins and D1 protein (a thylakoid-bound protein) were correlated to N remobilization, except for the genotype Samouraï which showed a low NRE despite high levels of proteolysis. Under restricted nitrate conditions, high levels of soluble protein degradation were associated with serine, cysteine and aspartic proteases at acidic pH. Low leaf NRE was related to a weak proteolysis of both soluble and thylakoid-bound proteins. The results obtained on the genotype Samouraï suggest that the timing between the onset of proteolysis and abscission could be a determinant. The specific involvement of acidic proteases suggests that autophagy and/or senescence-associated vacuoles are implicated in N remobilization under low N conditions. The data revealed that the rate of D1 degradation could be a relevant indicator of leaf NRE and might be used as a tool for plant breeding. PMID:27135221

  18. Introgression between oilseed rape (Brassica napus L.) and its weedy relative B. rapa L. in a natural population

    DEFF Research Database (Denmark)

    Hansen, L.B.; Siegismund, Hans Redlef; Jørgensen, R.B.

    2001-01-01

    .napus-specific markers. We compared thenumber of markers in the plants from the weedy population with thenumbers in controlled backcross generations (BC1 andBC2). The marker distribution in the weedy populationresembled the distribution in the second backcross generation mostclosely. Together with the cultivation......We investigated introgression in a mixed weedy population ofoilseed rape (Brassica napus) and itsrelative B. rapa usingspecies-specific AFLP-markers. The population wassituated in a field relayed from conventional to organic cultivation11 years ago. One-hundred-and-twoB. napus orB. rapa......-like plantswere collected in a 3 m2 plot. Of these, onewas a first generation hybrid (F1) and nearlyhalf (44 plants) were introgressed, having bothB. napus andB. rapa specific markers.The remaining plants apparently corresponded to pure species, with 50having only B. rapa-and seven having only B...

  19. Metabolic network reconstruction and flux variability analysis of storage synthesis in developing oilseed rape (Brassica napus L.) embryos

    Energy Technology Data Exchange (ETDEWEB)

    Hay, J.; Schwender, J.

    2011-08-01

    Computational simulation of large-scale biochemical networks can be used to analyze and predict the metabolic behavior of an organism, such as a developing seed. Based on the biochemical literature, pathways databases and decision rules defining reaction directionality we reconstructed bna572, a stoichiometric metabolic network model representing Brassica napus seed storage metabolism. In the highly compartmentalized network about 25% of the 572 reactions are transport reactions interconnecting nine subcellular compartments and the environment. According to known physiological capabilities of developing B. napus embryos, four nutritional conditions were defined to simulate heterotrophy or photoheterotrophy, each in combination with the availability of inorganic nitrogen (ammonia, nitrate) or amino acids as nitrogen sources. Based on mathematical linear optimization the optimal solution space was comprehensively explored by flux variability analysis, thereby identifying for each reaction the range of flux values allowable under optimality. The range and variability of flux values was then categorized into flux variability types. Across the four nutritional conditions, approximately 13% of the reactions have variable flux values and 10-11% are substitutable (can be inactive), both indicating metabolic redundancy given, for example, by isoenzymes, subcellular compartmentalization or the presence of alternative pathways. About one-third of the reactions are never used and are associated with pathways that are suboptimal for storage synthesis. Fifty-seven reactions change flux variability type among the different nutritional conditions, indicating their function in metabolic adjustments. This predictive modeling framework allows analysis and quantitative exploration of storage metabolism of a developing B. napus oilseed.

  20. Different waves of effector genes with contrasted genomic location are expressed by Leptosphaeria maculans during cotyledon and stem colonization of oilseed rape.

    Science.gov (United States)

    Gervais, Julie; Plissonneau, Clémence; Linglin, Juliette; Meyer, Michel; Labadie, Karine; Cruaud, Corinne; Fudal, Isabelle; Rouxel, Thierry; Balesdent, Marie-Hélène

    2017-10-01

    Leptosphaeria maculans, the causal agent of stem canker disease, colonizes oilseed rape (Brassica napus) in two stages: a short and early colonization stage corresponding to cotyledon or leaf colonization, and a late colonization stage during which the fungus colonizes systemically and symptomlessly the plant during several months before stem canker appears. To date, the determinants of the late colonization stage are poorly understood; L. maculans may either successfully escape plant defences, leading to stem canker development, or the plant may develop an 'adult-stage' resistance reducing canker incidence. To obtain an insight into these determinants, we performed an RNA-sequencing (RNA-seq) pilot project comparing fungal gene expression in infected cotyledons and in symptomless or necrotic stems. Despite the low fraction of fungal material in infected stems, sufficient fungal transcripts were detected and a large number of fungal genes were expressed, thus validating the feasibility of the approach. Our analysis showed that all avirulence genes previously identified are under-expressed during stem colonization compared with cotyledon colonization. A validation RNA-seq experiment was then performed to investigate the expression of candidate effector genes during systemic colonization. Three hundred and seven 'late' effector candidates, under-expressed in the early colonization stage and over-expressed in the infected stems, were identified. Finally, our analysis revealed a link between the regulation of expression of effectors and their genomic location: the 'late' effector candidates, putatively involved in systemic colonization, are located in gene-rich genomic regions, whereas the 'early' effector genes, over-expressed in the early colonization stage, are located in gene-poor regions of the genome. © 2016 BSPP AND JOHN WILEY & SONS LTD.

  1. Oilseed rape grain yield productivity increases with hybrid varietal types: a first balance sheet with post registration tests in France and in Europe

    Directory of Open Access Journals (Sweden)

    Pinochet Xavier

    2000-01-01

    Full Text Available Since 1994 several oilseed rape hybrid types were proposed to farmers. Following registration experiments, Cetiom and different equivalent institutions in European Union have tested them in different post registration national networks. Grain yield productivity increases were demonstrated and a first synthesis could be done to check avantages and difficulties which had occurred. For winter types, Hybrid Composits were widely used, mainly in France and in the United Kingdom. Grain yield increases were important in the South and West part of France where their market shares increased up to 50-80%. Nevertheless, many fecondation problems occurred in several places all over Europe. Reasons of such problems were difficult to identify. Several factors, as cold temperatures, nutritionnal competitions, pollen avaibility may be involved to explain low seed sets. Restored Hybrids made with the NPZ hybridation system were successfully tested widely, and has reached significative market shares during 1999-2000 season. Less experiments were carried out with others hybrid types (Ogu-INRA Restored Hybrids, Mixed Hybrids. Ogu-INRA Restored Hybrids reached the highest grain yield levels but users are waiting for lower glucosinolates seed content hybrids which would come in the next future. Performances comparisons among countries have to be done carefully. Productivity increases with hybrid types could have been over estimated depending of pollinic environments or plot size and possible neighbouring effects. For France, results from field trials networks are coherent with results coming from postal surveys. For spring varieties, Polima restored hybrids and varietal associations have demonstrated a significative advantage compared to classical lines.

  2. Differences between winter oilseed rape (Brassica napus L.) cultivars in nitrogen starvation-induced leaf senescence are governed by leaf-inherent rather than root-derived signals

    Science.gov (United States)

    Koeslin-Findeklee, Fabian; Becker, Martin A.; van der Graaff, Eric; Roitsch, Thomas; Horst, Walter J.

    2015-01-01

    Nitrogen (N) efficiency of winter oilseed rape (Brassica napus L.) line-cultivars (cvs.), defined as high grain yield under N limitation, has been primarily attributed to maintained N uptake during reproductive growth (N uptake efficiency) in combination with delayed senescence of the older leaves accompanied with maintained photosynthetic capacity (functional stay-green). However, it is not clear whether genotypic variation in N starvation-induced leaf senescence is due to leaf-inherent factors and/or governed by root-mediated signals. Therefore, the N-efficient and stay-green cvs. NPZ-1 and Apex were reciprocally grafted with the N-inefficient and early-senescing cvs. NPZ-2 and Capitol, respectively and grown in hydroponics. The senescence status of older leaves after 12 days of N starvation assessed by SPAD, photosynthesis and the expression of the senescence-specific cysteine protease gene SAG12-1 revealed that the stay-green phenotype of the cvs. NPZ-1 and Apex under N starvation was primarily under the control of leaf-inherent factors. The same four cultivars were submitted to N starvation for up to 12 days in a time-course experiment. The specific leaf contents of biologically active and inactive cytokinins (CKs) and the expression of genes involved in CK homeostasis revealed that under N starvation leaves of early-senescing cultivars were characterized by inactivation of biologically active CKs, whereas in stay-green cultivars synthesis, activation, binding of and response to biologically active CKs were favoured. These results suggest that the homeostasis of biologically active CKs was the predominant leaf-inherent factor for cultivar differences in N starvation-induced leaf senescence and thus N efficiency. PMID:25944925

  3. Differences between winter oilseed rape (Brassica napus L.) cultivars in nitrogen starvation-induced leaf senescence are governed by leaf-inherent rather than root-derived signals.

    Science.gov (United States)

    Koeslin-Findeklee, Fabian; Becker, Martin A; van der Graaff, Eric; Roitsch, Thomas; Horst, Walter J

    2015-07-01

    Nitrogen (N) efficiency of winter oilseed rape (Brassica napus L.) line-cultivars (cvs.), defined as high grain yield under N limitation, has been primarily attributed to maintained N uptake during reproductive growth (N uptake efficiency) in combination with delayed senescence of the older leaves accompanied with maintained photosynthetic capacity (functional stay-green). However, it is not clear whether genotypic variation in N starvation-induced leaf senescence is due to leaf-inherent factors and/or governed by root-mediated signals. Therefore, the N-efficient and stay-green cvs. NPZ-1 and Apex were reciprocally grafted with the N-inefficient and early-senescing cvs. NPZ-2 and Capitol, respectively and grown in hydroponics. The senescence status of older leaves after 12 days of N starvation assessed by SPAD, photosynthesis and the expression of the senescence-specific cysteine protease gene SAG12-1 revealed that the stay-green phenotype of the cvs. NPZ-1 and Apex under N starvation was primarily under the control of leaf-inherent factors. The same four cultivars were submitted to N starvation for up to 12 days in a time-course experiment. The specific leaf contents of biologically active and inactive cytokinins (CKs) and the expression of genes involved in CK homeostasis revealed that under N starvation leaves of early-senescing cultivars were characterized by inactivation of biologically active CKs, whereas in stay-green cultivars synthesis, activation, binding of and response to biologically active CKs were favoured. These results suggest that the homeostasis of biologically active CKs was the predominant leaf-inherent factor for cultivar differences in N starvation-induced leaf senescence and thus N efficiency. © The Author 2015. Published by Oxford University Press on behalf of the Society for Experimental Biology.

  4. Effects of Cycocel and Nitrogen Application on Yield and Yield Components of Autumn-Grown Oilseed Rape at Different Plant Densities

    Directory of Open Access Journals (Sweden)

    S. Majd

    2013-06-01

    Full Text Available In this research, which was carried out as two experiments (in the field and greenhouse at Research Farm of College of Agriculture, Shiraz University, Shiraz, Iran, in 2009-2010 growing season, the effects of different levels of nitrogen, plant density and cycocel application on yield and yield components of autumn-grown oilseed rape (Talaye cultivar were investigated. The field experiment was designed as split-split plot based on completely randomized blocks design. Treatments included nitrogen level (80, 140, 200 and 260 kg/ha as the main plot, plant density (70 and 90 plants/m2 as sub-plot and cycocel rate (0, 1.4 and 2.8 L/ha as sub- subplot. The greenhouse experiment, which was arranged as a factorial based on complete randomized design, included nitrogen level (0, 50, 100, 150 and 200 kg/ha and cycocel rate (0, 1.4 and 2.8 L/ha. Results showed that the highest seed yield was achieved at 200 kg N/ha, 2.8 L/ha cycocel and plant density of 90 plants/m2 (533.17, 533.96 and 521.6 g/m2, respectively. Application of 2.8 L/ha cycocel was associated with increased number of siliques per plant and final plants dry weight. Increasing plant density from 70 to 90 plants/m2 was associated with decreased number of siliques per plant, plant dry weight and number of seeds per silique. It appears that application of 200 kg N/ha, 2.8 L/ha cycocel and plant density of 90 plants/m2 could be recommended for maximum grain yield of autumn-grown rapeseed cv. Talaye, in agroclimatic conditions similar to this research

  5. A comparative investigation of the metabolism of the herbicide glufosinate in cell cultures of transgenic glufosinate-resistant and non-transgenic oilseed rape (Brassica napus) and corn (Zea mays).

    Science.gov (United States)

    Ruhland, Monika; Engelhardt, Gabriele; Pawlizki, Karlheinz

    2002-10-01

    To obtain information on differences between the metabolic pathways of the herbicide glufosinate (trade names: BASTA, LIBERTY) in non-transgenic, glufosinate-sensitive plants and in transgenic, glufosinate-resistant plants, the metabolism of 14C-labeled glufosinate and its enantiomers L- and D-glufosinate was studied using cell cultures of oilseed rape and corn. Transformation of glufosinate in both sensitive and transgenic rape cells remained at a low rate of about 3-10% in contrast to corn cells, where 20% was transformed in sensitive and 43% in transgenic cells after 14 days of incubation, the rest remaining as unchanged glufosinate. In sensitive rape and corn cells the main metabolite was 4-methylphosphinico-2-oxo-butanoic acid (PPO) with 7.3 and 16.4%, respectively, together with low amounts of 3-methylphosphinicopropionic acid (MPP), 4-methylphosphinico-2-hydroxybutanoic acid (MHB), 4-methylphosphinicobutanoic acid (MPB) and 2-methylphosphinicoacetic acid (MPA). An additional metabolite formed in transgenic cell cultures was 2-acetamido-4-methylbutanoic acid (N-acetyl-L-glufosinate, NGA), which was formed at rates of 3.2% in rape and 16.1% in corn. A further minor metabolite, not yet identified, was detected in both cell types. The liberation of 0.2% 14CO2 indicates further metabolic steps prior to a limited mineralization in plant cell cultures. L-glufosinate was transformed into the same metabolites as the glufosinate racemate. D-glufosinate was not metabolized.

  6. Heat stress during seed filling interferes with sulfur restriction on grain composition and seed germination in oilseed rape (Brassica napus L.

    Directory of Open Access Journals (Sweden)

    Sophie eBrunel-Muguet

    2015-04-01

    Full Text Available In coming decades, increasing temperatures are expected to impact crop yield and seed quality. To develop low input systems, the effects of temperature and sulfur (S nutrition in oilseed rape, a high S demanding crop, need to be jointly considered. In this study, we investigated the effects of temperatures (High Temperature (HT, 33 °C/day, 19 °C/night vs. Control Temperature (Ctrl T, 20°C/day, 15°C/day and S supply (High S (HS, 500 µm SO42- vs. Low S (LS, 8.7 µM SO42- during seed filling on (i yield components (seed number, seed dry weight (SDW and seed yield, (ii grain composition (nitrogen (N and S contents and quality (fatty acid (FA composition and seed storage protein (SSP accumulation and (iii germination characteristics (pre-harvest sprouting, germination rates and abnormal seedlings. Abscisic acid (ABA, soluble sugar contents and seed conductivity were also measured. HT and LS decreased the number of seeds per plant. SDW was less affected due to compensatory effects since the number of seeds decreased under stress conditions. While LS had negative effects on seed composition by reducing the FA contents and increasing the ratio S-poor SSPs (12S globulins/S-rich SSPs (2S albumins ratio, HT had positive effects by increasing S and FA contents and decreasing the C18:2/C18:3 ratio and the 12S/2S protein ratio. Seeds produced under HT showed high pre-harvest sprouting rates along with decreased ABA contents and high rates of abnormal seedlings. HT and LS restriction significantly accelerated germination times. High conductivity, which indicates poor seed storage capacity, was higher in HT seeds. Consistently, the lower ratio of (raffinose +stachyose/sucrose in HT seeds indicated low seed storage capacity. We demonstrated the effects of HT and LS on grain and on germination characteristics. These results suggest that hormonal changes might control several seed characteristics simultaneously.

  7. High-throughput phenotyping (HTP) identifies seedling root traits linked to variation in seed yield and nutrient capture in field-grown oilseed rape (Brassica napus L.).

    Science.gov (United States)

    Thomas, C L; Graham, N S; Hayden, R; Meacham, M C; Neugebauer, K; Nightingale, M; Dupuy, L X; Hammond, J P; White, P J; Broadley, M R

    2016-04-06

    Root traits can be selected for crop improvement. Techniques such as soil excavations can be used to screen root traits in the field, but are limited to genotypes that are well-adapted to field conditions. The aim of this study was to compare a low-cost, high-throughput root phenotyping (HTP) technique in a controlled environment with field performance, using oilseed rape (OSR;Brassica napus) varieties. Primary root length (PRL), lateral root length and lateral root density (LRD) were measured on 14-d-old seedlings of elite OSR varieties (n = 32) using a 'pouch and wick' HTP system (∼40 replicates). Six field experiments were conducted using the same varieties at two UK sites each year for 3 years. Plants were excavated at the 6- to 8-leaf stage for general vigour assessments of roots and shoots in all six experiments, and final seed yield was determined. Leaves were sampled for mineral composition from one of the field experiments. Seedling PRL in the HTP system correlated with seed yield in four out of six (r = 0·50, 0·50, 0·33, 0·49;P < 0·05) and with emergence in three out of five (r = 0·59, 0·22, 0·49;P < 0·05) field experiments. Seedling LRD correlated positively with leaf concentrations of some minerals, e.g. calcium (r = 0·46;P < 0·01) and zinc (r = 0·58;P < 0·001), but did not correlate with emergence, general early vigour or yield in the field. Associations between PRL and field performance are generally related to early vigour. These root traits might therefore be of limited additional selection value, given that vigour can be measured easily on shoots/canopies. In contrast, LRD cannot be assessed easily in the field and, if LRD can improve nutrient uptake, then it may be possible to use HTP systems to screen this trait in both elite and more genetically diverse, non-field-adapted OSR. © The Author 2016. Published by Oxford University Press on behalf of the Annals of Botany Company.

  8. Reduction of selection pressure of herbicides - options and limits for blackgrass management by using clethodim in oilseed rape in the presence of the Leu1781 haplotype

    Directory of Open Access Journals (Sweden)

    Wagner, Jean

    2014-02-01

    Full Text Available In field experiments the control of blackgrass (Alopecurus myosuroides, Huds. in oilseed rape using clethodim (Select EC 240 and cycloxydim (Focus Ultra with and without subsequent treatments with propyzamide (Kerb FLO was tested at 6 locations in North and South Germany with assumed resistance problems. The field experiments were accompanied using molecular analysis leaf samples from the plots to seize the occurrence of black-grass with the Leu 1781 haplotype and to determine the frequency of the genotypes. The goal of the trials was to correlate the successes of blackgrass control with the occurrence of hetero- and homozygous resistant genotypes. It was shown in greenhouse trials that clethodim selects the haplotype Leu1781 more weakly (and it shows a higher partial efficacy than cycloxydim and that heterozygous plants have a lower resistance factor than homozygous plants. The question raised whether the frequency of heterozygous plants has influence on increased efficacy of clethodim under field conditions. At 5 sites target-site resistance was detected. At one location the high proportion of heterozygous plants correlated positive with relative higher control using Select EC 240 (80% compared to Focus Ultra (0%. At two locations with high proportion of homozygous resistant plants Select EC 240 and Focus Ultra treatments without subsequent treatments with Kerb FLO were not sufficient in solo variants. The subsequent treatments with Kerb FLO provided partly, but not sufficient control of black-grass. At one location no resistance was identified. The effect of Select EC 240 and Focus Ultra to control black-grass were comparable high in all variants with and without subsequent treatments of Kerb FLO. The investigations showed clearly a higher degree of control by plants with the haplotype Leu1781 by the active substance clethodim and pointed out the fact that the frequency of resistant genotypes (homo vs. heterozygous resistant plants has a

  9. Detecting management and fertilization effects on the carbon balance of winter oilseed rape with manual closed chamber measurements: Can we outrange gap-filling uncertainty and spatiotemporal variability?

    Science.gov (United States)

    Huth, Vytas; Moffat, Antje Maria; Calmet, Anna; Andres, Monique; Laufer, Judit; Pehle, Natalia; Rach, Bernd; Gundlach, Laura; Augustin, Jürgen

    2017-04-01

    Winter oilseed rape is the dominant biofuel crop in the young moraine landscape in North-Eastern Germany. However, studies on the effect of rapeseed cropping on net ecosystem exchange of CO2 (NEE) and the soil carbon (SC) balance are scarce. SC balance estimates are usually derived from long-term soil sampling field trials where rapeseed is part of different crop rotations. The estimated annual differences linked to rapeseed cropping are rather small (varying between losses of 40 g C m-2 and gains of up to 100 g C m-2). Testing management effects on the NEE and SC balance of cropping systems is best done by comparing plots with different treatments at the same site under the same climate. The soil sampling approach is in the need of field trials that run over decades, which has the disadvantage that management strategies of practical farming may have already changed when the results are derived. Continuous eddy covariance measurements of NEE would require large fields in flat terrain for each of the treatments, which is especially complicated in the heterogeneous landscapes of glacigenic origin of North-Eastern Germany. The common approach of using the chamber technique to derive NEE, however, is subject to the local soil and plant stand heterogeneities due to its tiny footprint. This technique might also disturb the ecosystem, the measurements are usually discontinuous requiring elaborate gap-filling techniques, and it has mostly been used on organic soils where large respiratory C losses occur. Therefore, our aim was to answer, if a combined approach of the eddy covariance and the chamber technique can detect the relatively small NEE and SC differences of rapeseed cropping on mineral soils within a shorter period of time than conventional soil sampling field trials can. We tested the new experimental design taking the advantages of both techniques into account: The eddy covariance tower measuring the NEE dynamics during the year; the chamber measurements to

  10. Male fitness of oilseed rape (¤Brassica napus¤), weedy ¤B-rapa¤ and their F1 hybrids when pollinating ¤B-rapa¤ seeds

    DEFF Research Database (Denmark)

    Pertl, M.; Hauser, T.P.; Damgaard, C.

    2002-01-01

    ) and their F(1) hybrids at different frequencies and densities. Paternity was determined by the presence of a transgene, morphology, and AFLP markers. In addition, observations of flower and pollen production, and published data on pollen fertilisation success, zygote survival, and seed germination, allowed us......The likelihood that two species hybridise and backcross may depend strongly on environmental conditions, and possibly on competitive interactions between parents and hybrids. We studied the paternity of seeds produced by weedy Brassica rapa growing in mixtures with oilseed rape (B. napus...... to estimate an expected paternity. The frequency and density of B. napus, B. rapa, and F(1) plants had a strong influence on flower, pollen, and seed production, and on the paternity of B. rapa seeds. Hybridisation and backcrossing mostly occurred at low densities and at high frequencies of B. napus and F(1...

  11. The effect of phosphours and water deficit on phosphatase activity and proline accumulation in seedling cotyledons and roots of oilseed rape as compared to that of excised cotyledons and roots

    Directory of Open Access Journals (Sweden)

    Stanisław Flasiński

    2014-01-01

    Full Text Available Oilseed rape seedlings and excised cotyledons and roots were exposed to phosphorus and osmotic stress (-1 MPa: NaCl or PEG. The stress factors limited the growth of the seedlings and inhibited the growth of the excised roots and cotyledons. The phosphorus content in the cotyledons and roots depended on its level in the media and on the stress factors used. Phosphorus deficiency differentiated total phosphatase activity in seedling cotyledons and increased the activity in the excised cotyledons. In the excised and seedling roots, the lack of phosphorus, its deficiency and stress imposed by the addition of NaCl, caused an increase in total and specific phosphatase activity. Osmotic stress caused proline accumulation in both the seedling and excised cotyledons, but the effect of phosphorus stress was much smaller. Proline increase in seedling roots followed only after the osmotic stress caused by NaCl. The proline content in the excised roots was low. Stress factors lowered the protein content in the seedling and excised cotyledons. In both seedling and excised roots, the lowering of the protein content occurred mainly in response to osmotic stress. Kinetin modified metabolic responses in seedling cotyledons and roots. The use of in vitro culture allowed the elucidation of the tested responses. In excised cotyledons grown under stress conditions, kinetin increased the phosphorus and protein contents and lowered proline accumulation, suggesting that kinetin had the ability to overcome phosphorus and osmotic stress. The excised oilseed rape roots responded only slightly to kinetin (Drozdowska and Rogozińska 1984 and its effect was much smaller.

  12. Identification and characterization of microRNAs in oilseed rape (Brassica napus) responsive to infection with the pathogenic fungus Verticillium longisporum using Brassica AA (Brassica rapa) and CC (Brassica oleracea) as reference genomes.

    Science.gov (United States)

    Shen, Dan; Suhrkamp, Ina; Wang, Yu; Liu, Shenyi; Menkhaus, Jan; Verreet, Joseph-Alexander; Fan, Longjiang; Cai, Daguang

    2014-11-01

    Verticillium longisporum, a soil-borne pathogenic fungus, causes vascular disease in oilseed rape (Brassica napus). We proposed that plant microRNAs (miRNAs) are involved in the plant-V. longisporum interaction. To identify oilseed rape miRNAs, we deep-sequenced two small RNA libraries made from V. longisporum infected/noninfected roots and employed Brassica rapa and Brassica oleracea genomes as references for miRNA prediction and characterization. We identified 893 B. napus miRNAs representing 360 conserved and 533 novel miRNAs, and mapped 429 and 464 miRNAs to the AA and CC genomes, respectively. Microsynteny analysis with the conserved miRNAs and their flanking protein coding sequences revealed 137 AA-CC genome syntenic miRNA pairs and 61 AA and 42 CC genome-unique miRNAs. Sixty-two miRNAs were responsive to the V. longisporum infection. We present data for specific interactions and simultaneously reciprocal changes in the expression levels of the miRNAs and their targets in the infected roots. We demonstrate that miRNAs are involved in the plant-fungus interaction and that miRNA168-Argonaute 1 (AGO1) expression modulation might act as a key regulatory module in a compatible plant-V. longisporum interaction. Our results suggest that V. longisporum may have evolved a virulence mechanism by interference with plant miRNAs to reprogram plant gene expression and achieve infection. © 2014 The Authors. New Phytologist © 2014 New Phytologist Trust.

  13. Simultaneous determination of 13 phytohormones in oilseed rape tissues by liquid chromatography-electrospray tandem mass spectrometry and the evaluation of the matrix effect.

    Science.gov (United States)

    Fan, Sufang; Wang, Xiupin; Li, Peiwu; Zhang, Qi; Zhang, Wen

    2011-03-01

    In the experiment, a high-performance liquid chromatography and electrospray ionization-tandem mass spectrometry with selected reaction monitoring was used to simultaneously determine various classes of phytohormones, including indole-3-acetic acid, α-naphthaleneacetic acid, 2-chlorobenzoic acid, 4-chlorobenzoic acid, indole-3-butyric acid, gibberellic acid, 2,4-dichlorophenoxyacetic acid, 2-naphthoxyacetic acid, abscisic acid, 2,3,5-triiodobenzoic acid, uniconazole, paclobutrazol and 2,4-epibassinolide in rape tissues. The analyses were separated by an HPLC equipped with a reversed-phase column using a binary solvent system composed of methanol and water, both containing 0.1% of formic acid. The matrix effect was also considered and determined. The technology was applied to analyze rape tissues, including roots, stems, leaves, flowers, immature pods and rape seeds. The rape tissues were subjected to ultrasound-assisted extraction and purified by dispersive solid-phase extraction, and then transferred into the liquid chromatography system. The detection limit for each plant hormone was defined by the ratio of signal/background noise (S/N) of 3. The results showed perfect linearity (R(2) values of 0.9987-1.0000) and reproducibility of elution times (relative standard deviations, RSDs,<1%) and peak areas (RSDs,<7%) for all target compounds. Copyright © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  14. A systemic increase in the recombination frequency upon local infection of Arabidopsis thaliana plants with oilseed rape mosaic virus depends on plant age, the initial inoculum concentration and the time for virus replication

    Directory of Open Access Journals (Sweden)

    Youli eYao

    2013-03-01

    Full Text Available In the past, we showed that local infection of tobacco leaves with either Tobacco mosaic virus (TMV or Oilseed rape mosaic virus (ORMV resulted in a systemic increase in the homologous recombination frequency (HRF. Later on, we showed that a similar phenomenon occurs in Arabidopsis thaliana plants infected with ORMV. Here, we tested whether the time of removing the infected leaves as well as viral titer have any effect on the degree of changes in HRF in systemic tissues. An increase in HRF in systemic non-infected tissues was more pronounced when the infected leaves were detached from the infected plants at 60-96 hours post infection, rather than at earlier time. Next, we found that exposure to higher concentrations of inoculum was much more efficient in triggering an increase in HRF than exposure to lower concentrations. Finally, we showed that older plants exhibited a higher increase in HRF than younger plants. We found that an increase in genome instability in systemic tissues of locally infected plants depends on plant age, the concentration of initial inoculums and the time of viral replication.

  15. Comparison of a diurnal vs steady-state ozone exposure profile on growth and yield of oilseed rape (Brassica napus L.) in open-top chambers in the Yangtze Delta, China

    Energy Technology Data Exchange (ETDEWEB)

    Wang Xiaoke [State Key Laboratory of Urban and Regional Ecology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Shuangqinglu 18, Beijing 100085 (China)], E-mail: wangxk@rcees.ac.cn; Zheng Qiwei; Feng Zhaozhong [State Key Laboratory of Urban and Regional Ecology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Shuangqinglu 18, Beijing 100085 (China); Xie Juqing [Northwestern Sci-Tech University of Agriculture and Forestry, Yangling, Shaanxi 712100 (China); Feng Zongwei; Ouyang Zhiyun [State Key Laboratory of Urban and Regional Ecology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Shuangqinglu 18, Beijing 100085 (China); Manning, William J. [Department of Plant, Soil and Insect Sciences, University of Massachusetts, Amherst, MA 01003-9320 (United States)

    2008-11-15

    Most available exposure-response relationships for assessing crop loss due to elevated ozone (O{sub 3}) have been established using data from chamber and open-top chamber experiments, using a simulated constant O{sub 3} concentration exposure (square wave), which is not consistent with the diurnal variation of O{sub 3} concentration that occurs in nature. We investigated the response of oilseed rape (Brassica napus L.) to O{sub 3} as affected by two exposure regimes: one with a diurnal variation (CF100D) and another with a constant concentration (CF100). Although the two exposure regimes have the same mean O{sub 3} concentration and accumulated O{sub 3} concentration above 40 ppb (AOT40), our results show that O{sub 3} at CF100D reduced biomass and number of pods/plant more than O{sub 3} at CF100. Both O{sub 3} exposures resulted in larger seed weights/100 pods compared to CF. Numbers of seeds/100 pods were reduced by CF100, while numbers of seeds/100 pods in the CF100D chambers were comparable to those in CF. Our results suggest that chamber experiments that use a constant O{sub 3} exposure may underestimate O{sub 3} effects on biomass and yields. - Diurnal variation of O{sub 3} concentration should be considered when designing O{sub 3} exposure experiment.

  16. Climate change and genetically modified insecticidal plants. Plant-herbivore interactions and secondary chemistry of Bt Cry1Ac-toxin producing oilseed rape (Brassica napus L.) under elevated CO{sub 2} or O{sub 3}

    Energy Technology Data Exchange (ETDEWEB)

    Himanen, S.

    2008-07-01

    Transgenic insect-resistant plants producing Bacillus thuringiensis (Bt) crystalline endotoxins are the first commercial applications of genetically modified crops and their use has steadily expanded over the last ten years. Together with the expanding agricultural use of transgenic crops, climate change is predicted to be among the major factors affecting agriculture in the coming years. Plants, herbivores and insects of higher trophic levels are all predicted to be affected by the current atmospheric climate change. However, only very few studies to date have addressed the sustained use and herbivore interactions of Bt-producing plants under the influence of these abiotic factors. The main objective of this study was to comparatively assess the performance of a Bt Cry1Ac toxin-producing oilseed rape line and its non-transgenic parent line in terms of vegetative growth and allocation to secondary defence compounds (glucosinolates and volatile terpenoids), and the performance of Bt-target and nontarget insect herbivores as well as tritrophic interaction functioning on these lines. For this, several growth chamber experiments with vegetative stage non-Bt and Bt plants facing exposures to doubled atmospheric CO{sub 2} level alone or together with increased temperature and different regimes of elevated O{sub 3} were conducted. The main hypothesis of this work was that Bt-transgenic plants have reduced performance or allocation to secondary compounds due to the cost of producing Bt toxin under changed abiotic environments. The Bt-transgenic oilseed rape line exhibited slightly delayed vegetative growth and had increased nitrogen and reduced carbon content compared to the non-transgenic parent line, but the physiological responses (i.e. biomass gain and photosynthesis) of the plant lines to CO{sub 2} and O{sub 3} enhancements were equal. Two aphid species, non-susceptible to Bt Cry1Ac, showed equal performance and reproduction on both plant lines under elevated CO{sub 2

  17. Distribución de nitrógeno, fósforo y azufre en un cultivo de colza: efectos sobre el ciclado de nutrientes Distribution of nitrogen, phosphorus and sulfur in oilseed rape: effects on nutrient cycling

    Directory of Open Access Journals (Sweden)

    Gerardo Rubio

    2007-12-01

    Full Text Available Comparado con otros cultivos, la colza tiene una alta demanda de azufre (S por lo que sería esperable que la inclusión de este cultivo en la rotación agrícola acelere el agotamiento de este nutriente en los suelos de las áreas cultivadas. En este trabajo, se comparan los patrones de partición de biomasa, S, nitrógeno (N y fósforo (P en plantas maduras de colza. La información a obtener es relevante desde el punto de vista del ciclado de nutrientes. Para ello se realizó un experimento de campo que se ajustó a un arreglo factorial con dos factores (N y S. En el momento de la cosecha, se midió la acumulación de biomasa, N, P y S en tres compartimientos: granos, rastrojo (resto de parte aérea y raíces. Aunque el rendimiento fue afectado levemente por la adición individual de N o S, la simultánea adición de ambos nutrientes provocó un incremento del 56%. El N y el P presentaron una distribución semejante entre los órganos de la planta estudiados, sin embargo, el S difirió marcadamente de ambos. Su partición al órgano que se exporta (granos fue de menor magnitud que la observada para N y P. En cambio, su partición al rastrojo en pie fue mayor. Esta característica atenuaría los efectos de la alta demanda de S sobre la exportación del cultivo y permitiría una reutilización del fertilizante agregado por el cultivo siguiente.Oilseed rape poses a higher sulfur (S demand, compared to other crops. This may indicate that the inclusion of this crop in the crop rotation could accelerate soil S depletion. In this work, we compared the allocation of biomass, nitrogen (N, phosphorus (P and S in oilseed rape mature plants. Two factors were analyzed in a field experiment: nitrogen and sulphur (two levels for each factor. At harvest, we measured the accumulation of biomass, N, P and S in three compartments: roots, straw and grains. Yield was little affected by the addition of single nutrients but the simultaneous addition of N and S

  18. How to model and simulate the effects of cropping systems on population dynamics and gene flow at the landscape level: example of oilseed rape volunteers and their role for co-existence of GM and non-GM crops.

    Science.gov (United States)

    Colbach, Nathalie

    2009-05-01

    Agricultural landscapes comprise cultivated fields and semi-natural areas. Biological components of these compartments such as weeds, insect pests and pathogenic fungi can disperse sometimes over very large distances, colonise new habitats via insect flight, spores, pollen or seeds and are responsible for losses in crop yield (e.g. weeds, pathogens) and biodiversity (e.g. invasive weeds). The spatiotemporal dynamics of these biological components interact with crop locations, successions and management as well as the location and management of semi-natural areas such as roadverges. The objective of this investigation was to establish a modelling and simulation methodology for describing, analysing and predicting spatiotemporal dynamics and genetics of biological components of agricultural landscapes. The ultimate aim of the models was to evaluate and propose innovative cropping systems adapted to particular agricultural concerns. The method was applied to oilseed rape (OSR) volunteers playing a key role for the coexistence of genetically modified (GM) and non-GM oilseed rape crops, where the adventitious presence of GM seeds in non-GM harvests (AGMP) could result in financial losses for farmers and cooperatives. A multi-year, spatially explicit model was built, using field patterns, climate, cropping systems and OSR varieties as input variables, focusing on processes and cultivation techniques crucial for plant densities and pollen flow. The sensitivity of the model to input variables was analysed to identify the major cropping factors. These should be modified first when searching for solutions limiting gene flow. The sensitivity to model processes and species life-traits were analysed to facilitate the future adaptation of the model to other species. The model was evaluated by comparing its simulations to independent field observations to determine its domain of validity and prediction error. The cropping system study determined contrasted farm types, simulated

  19. Scientific Opinion on application EFSA‐GMO‐NL‐2013‐119 for authorisation of genetically modified glufosinate‐ammonium‐ and glyphosate‐tolerant oilseed rape MON 88302 × MS8 × RF3 and subcombinations independently of their origin, for food and feed uses, import and processing submitted in accordance with Regulation (EC) No 1829/2003 by Monsanto Company and Bayer CropScience

    OpenAIRE

    Naegeli, Hanspeter; Birch, Andrew Nicholas; Casacuberta, Josep; De Schrijver, Adinda; Gralak, Mikołaj Antoni; Guerche, Philippe; Jones, Huw; Manachini, Barbara; Messéan, Antoine; Nielsen, Elsa Ebbesen; Nogué, Fabien; Robaglia, Christophe; Rostoks, Nils; Sweet, Jeremy; Tebbe, Christoph

    2017-01-01

    In this opinion, the GMO Panel assessed the three-event stack oilseed rape (OSR) MON 88302 × MS8 × RF3 and its three subcombinations, independently of their origin. The GMO Panel has previously assessed the single events combined to produce this three-event stack OSR and did not identify safety concerns; no new information that would modify the original conclusions was identified. The combination of the single OSR events and of the newly expressed proteins in the three-event stack OSR does no...

  20. Date Rape (For Teens)

    Science.gov (United States)

    ... Breath? Talking to Your Parents - or Other Adults Date Rape KidsHealth > For Teens > Date Rape Print A ... Yourself Getting Help Reporting a Rape What Is Date Rape? When people think of rape , they might ...

  1. RESPONSE OF SIX OILSEED RAPE GENOTYPES TO WATER STRESS AND HYDROGEL APPLICATION RESPOSTA DE SEIS GENÓTIPOS DE CANOLA A ESTRESSE HÍDRICO E APLICAÇÃO DE HIDROGEL

    Directory of Open Access Journals (Sweden)

    M. Mashhadi-Akbar-Boojar

    2009-09-01

    Full Text Available

    The hydrogel amendments may improve seedling growth and establishment by increasing water retention capacity of soils and regulation of plants available water supplies, especially under arid environments. This study was conducted to evaluate the role of super absorbent polymer use in oilseed rape (Brassica napus L. genotypes, under drought stress, evaluating some agronomic (total biomass, seed yield, yield components and harvest index and physiological characters (chlorophyll content. The experiment was carried out in the research farm of the Seed and Plant Improvement Institute, in 2007-2008, in Karaj, Iran (35°59´N, 50°75E, 151 m altitude, as a randomized complete block design, with factorial split-plot arrangement, with three replications. The irrigation strategy had two levels (irrigation after 80% of water evaporation, from class A Pan as control, and drought stress, starting from the flowering stage to physiological maturity and the application of super absorbent also occurred at two levels (absence of super absorbent as control, and application of super absorbent at 7% concentration, as main plots. Genotypes Rgs003 (V1, Sarigol (V2, Option500 (V3, Hyola401 (V4, Hyola330 (V5, and Hyola420 (V6 were allotted to subplots. Results showed a significant difference between irrigation treatments, presence of super absorbent, and genotypes on agronomic and physiological characters. Water stress decreased total biomass, seed yield components, harvest index, and chlorophyll content. On the other hand, under field conditions, the use of 7% of super absorbent increased agronomic and

  2. Radiocaesium activity in rape oil and in rape cake after a wet deposition event

    Energy Technology Data Exchange (ETDEWEB)

    Chaiko, Y.; Rappe-George, M.; Bengtsson, S. [Department of Soil and Environment (Sweden)

    2014-07-01

    After a release of radionuclides in agricultural areas there can be concerns on the levels of radionuclides in food products produced. The uptake of radionuclides via the above ground plant parts is a very important transport route into the food chain for humans as caesium is relatively mobile inside plants. In the production of rape oil the use of a processing factor (Pf) is used to estimate the activity concentration of radioactivity in the final oil product based on the activity concentration in rape seeds. The processing factor has been estimated to be 0.004 for caesium in rape oil by IAEA, and is based on a limited numbers of studies (1). In this project we analysed the activity concentration of radiocaesium ({sup 134}Cs) in rape oil and in rape cake from rape seeds contaminated after a wet deposition event with {sup 134}Cs. With the information of activity concentration of {sup 134}Cs in rape oil and in rape cake, we calculated the Pf-value and confirm or suggest new enhanced Pf-value. We analysed the activity concentration of {sup 134}Cs in rape oil and in rape cake from spring oilseed rape seeds (Brassica napus L.) that had been contaminated experimentally by wet deposited {sup 134}Cs in an earlier experiment by Bengtsson et al. (2013). The estimation of activity concentration of {sup 134}Cs in rape oil and in rape cake was achieved by performing extraction of the oil (and other extractable compounds) from the seeds based on the Randall extraction method (Randall, 1974) using petroleum ether as extraction chemical. The extracted oil and the rape cake samples were weighed and the activity concentration was measured with High-Purity Germanium (HPGe) detectors. Pf-values were calculated by dividing the measured activity concentration in rape oil after extraction by the activity concentration in oilseed rape seeds before extraction. Results from the present study will be presented at the international conference on radioecology and environmental radioactivity

  3. Rape sentencing

    DEFF Research Database (Denmark)

    Ó Cathaoir, Katharina Eva

    This handbook conducts an analysis of the sentences imposed for rape by Irish courts. Part I examines The People (DPP) v. WD [2007] IEHC 310 by outlining the salient points of the decision, in particular the separation of rape sentences into categories of punishments. The mitigating and aggravati...... factors are also laid out. Part II analyses recent sentences for rape since 2007. All reported Court of Criminal Appeal (CCA) cases post The People (DPP) v. WD are included as well as a survey of two years of Irish Times reports (covering the period November 2010 to November 2012)....

  4. Proteomic Analysis of Pollen and Blossom Honey from Rape Seed Brassica Napus L.

    Directory of Open Access Journals (Sweden)

    Borutinskaitė Veronika

    2017-06-01

    Full Text Available In the study, honey from oilseed rape Brassica napus L., and both hand-collected (winter rape Visby and Cult and bee-collected pollen of oilseed rape were analyzed for their proteome content, in order to see if any plant proteins were present to allow the proteo-typing of the oilseed rape honey. Proteins were fractionated by two-dimensional gel electrophoresis (2DE, stained by Coomassie blue and then analyzed by mass spectrometry. All identified proteins were divided into few groups due to their biological function. In 2DE gels with separated proteins from blossom honey, only bee (Apis mellifera main proteins (Major royal jelly protein 1-5 and Glucosidase were found. So we analyzed all proteins using gel-free based analysis with the SYNAPT G2 high definition mass spectrometry. We identified proteins that were present in both oilseed rape pollen and honey (Bna, Polygalacturonase, Non-specific lipid-transfer protein, GAPDH and others. We believe that these proteins are important for the nutritional value of plant pollen-enriched honey and further research is required on honey and honeybee pollen protein.

  5. Research fields, challenges and opportunities in European oilseed crops breeding

    Directory of Open Access Journals (Sweden)

    Vincourt Patrick

    2014-11-01

    Full Text Available Due to the geographical specialization in oilseed world production, Europe has a major role to play in winter oilseed rape and sunflower breeding. Mainly based on the most recen t results, this review aims to identify the main research and breeding targets for these two crops, as seen through publications, with an attempt to suggest what are opportunities and challenges in these research fields. Growing a healthy and yielding crop remains the key driver for agronomic production. However sustainability and environmental profiles of the cultivar are now entering the field of play: The sustainability concern invested the field of resistance to diseases. Nitrogen use efficiency became an important target for Brassica napus, and crop resilience toward drought stresses is the way chosen in Helianthus annuus breeding for yield improvement. Significant advances are underway for quality traits, but the uncertainty on nutritional and industrial demand may explain why the product diversification remains low.

  6. Post-harvest N2O emissions were not affected by various types of oilseed straw incorporated into soil

    Science.gov (United States)

    Köbke, Sarah; Senbayram, Mehmet; Hegewald, Hannes; Christen, Olaf; Dittert, Klaus

    2015-04-01

    Oilseed rape post-harvest N2O emissions are seen highly critical as so far they are considered as one of the most crucial drawbacks in climate-saving bioenergy production systems. N2O emissions may substantially counterbalance the intended savings in CO2 emissions. Carbon-rich crop residues in conjunction with residual soil nitrate are seen as a key driver since they may serve as energy source for denitrification and, they may alter soil-borne N2O emissions. As oilseed rape straw is known to have high N/C ratio compared to other crop residues, its soil incorporation may specifically trigger post-harvest N2O emissions. Therefore, the aim of the present study was to determine post-harvest N2O emissions in soils amended with various types of oilseed rape straw (with different N/C ratio) and barley straw in field and incubation experiments. In the incubation experiment, oilseed rape or 15N labelled barley straw were mixed with soil at a rate of 1.3 t DM ha-1 and studied for 43 days. Treatments consisted of non-treated control soil (CK), 15N labelled barley straw (BST), oilseed rape straw (RST), 15N labelled barley straw + N (BST+N), or oilseed rape straw + N (RST+N). N fertilizer was applied to the soil surface as ammonium-nitrate at a rate of 100 kg N ha-1 and soil moisture was adjusted to 80% water-holding capacity. In the field experiment, during the vegetation period 15N labelled fertilizer (15NH415NO3) was used to generate 15N labelled oilseed rape straw (up to 5 at%). Here, the three fertilizer treatments consisted of 5 kg N ha-1 (RST-5), 150 kg N ha-1 (RST-150) and 180 kg N ha-1 (RST-180). Post-harvest N2O emissions were determined during the period of August 2013 to February 2014 by using static flux chambers. In the incubation trial, cumulative N2O emissions were 5, 29, 40 g N2O-N ha-1 148 days-1 in non-fertilized control, BST and RST treatments, respectively. Here, emissions were slightly higher in RST than BST (p

  7. Radiation and nitrogen use in wheat and oilseed rape crops

    NARCIS (Netherlands)

    Dreccer, M.F.

    1999-01-01

    Raising yield potential of crops with an efficient use of nutrients is imperative, given the prospects of increase in world population and the need to reduce environmental problems. Yield potential is proportional to the total biomass of a crop, which is highly responsive to nitrogen

  8. Oilseeds business opportunities in Ethiopia

    NARCIS (Netherlands)

    Wijnands, J.H.M.; Biersteker, J.; Hiel, R.

    2007-01-01

    In this study the business opportunities for Dutch companies in the Ethiopian oilseeds sector are explored. Opportunities for setting up sustainable export chains should mutually benefit Dutch companies as well as the Ethiopian oilseeds sector in particular and the Ethiopian society in general.

  9. Numerical estimation of deformation energy of selected bulk oilseeds in compression loading

    Science.gov (United States)

    Demirel, C.; Kabutey, A.; Herak, D.; Gurdil, G. A. K.

    2017-09-01

    This paper aimed at the determination of the deformation energy of some bulk oilseeds or kernels namely oil palm, sunflower, rape and flax in linear pressing applying the trapezoidal rule which is characterized by the area under the force and deformation curve.The bulk samples were measured at the initial pressing height of 60 mm with the vessel diameter of 60 mm where they were compressed under the universal compression machine at a maximum force of 200 kN and speed of 5 mm/min.Based on the compression test, the optimal deformation energy for recovering the oil was observed at a force of 163 kN where there was no seed/kernel cake ejection in comparison to the initial maximum force used particularly for rape and flax bulk oilseeds.This information is needed for analyzing the energy efficiency of the non-linear compression process involving a mechanical screw press or expeller.

  10. Rape and Seduction Scripts.

    Science.gov (United States)

    Ryan, Kathryn M.

    1988-01-01

    College students (N=18) wrote scripts about "typical" rape and "typical" seduction. Scripts were coded on 20 dimensions. Results showed that rape and seduction scripts were very different. Most subjects described blitz rape in which woman outdoors was attacked by male stranger. Seductions described were usually indoors and…

  11. Ukrainian cereals and oilseeds trade

    OpenAIRE

    Б. В. Духницький; Т. В. Сіліфонов

    2016-01-01

    Purpose. To analyze peculiarities of Ukrainian cereals and oilseeds trade, the situation on the world market, and determine future prospects of its development. Methods. Analysis and synthesis, comparative evaluation, graphic procedure. Results. The role and place of Ukraine in a total grain supply to the world market was determined. Ukraine is a world’s top ten grain producer. Among domestic agricultural products, cereals, oilseeds and sunflower oil are in the highest demand in the w...

  12. Secondary victims of rape

    DEFF Research Database (Denmark)

    Christiansen, Dorte Mølgaard; Bak, Rikke; Elklit, Ask

    2012-01-01

    Rape is often a very traumatic experience, which affects not only the primary victim (PV) but also his/her significant others. Studies on secondary victims of rape are few and have almost exclusively studied male partners of female rape victims. This study examined the impact of rape on 107...... secondary victims, including family members, partners, and friends of male and female rape victims. We found that many respondents found it difficult to support the PV and that their relationship with the PV was often affected by the assault. Furthermore, the sample showed significant levels...... of social support for the respondent, and feeling let down by others. The respondents were generally interested in friend-, family-, and partner-focused interventions, particularly in receiving education about how best to support a rape victim...

  13. Rape in Rural Bangladesh

    Directory of Open Access Journals (Sweden)

    Nowsher Ali

    2015-03-01

    Full Text Available Background: Rape is one of the silent brutal sexual offences in Bangladesh. Despite strong laws against it, the evil of rape continues to rise. Increasing trend of the silent cruel sexual offence (rape represents a major psychopath sexual disorder and public health problem and progress of the country. Objective: The aim of the study was to determine the pattern of alleged rape victims in a rural district of Bangladesh with the ultimate aim to create public awareness about the brutal crime. Materials and method: This retrospective study was carried out on 330 sexually assailed alleged rape victims’ report forms, who reported at Faridpur Medical College, Bangladesh from 2007 to 2011 for medical examination. Results: Among the study subjects maximum number (70.0% of alleged rape cases were under the age of 20 years. More than two-thirds (64.60% of the assailants were known to the victims, most of the incidents (64.20% occurred in the victims’ houses and nearby places. The study also revealed that minimum number of victims (14.20% reported within 24 hours for medical examination. Almost one fourth of the alleged rape cases were gang rape and no positive finding in favour of sexual intercourse was found in about three fourth (72.40% of cases. Conclusion: Public awareness about rape would be effective to report in due time with preserving the evidence of crime and modern techniques like DNA diagnosis may be of help to detect the assailant.

  14. Rape: an Asian perspective.

    Science.gov (United States)

    Nadesan, K

    2001-06-01

    Rape is one of the fastest growing violent crimes in many parts of the world. Rape laws have been amended in most countries in an attempt to cope with the proliferation of this crime. Even though the legal definition of rape and the procedural laws have been amended, rape remains a serious problem in both the developed and developing nations. In some countries the offence of rape carries severe punishment sometimes even the death sentence. In many jurisdictions the term 'sexual penetration' is being used instead of 'sexual intercourse'. Sexual penetration includes sexual intercourse, anal intercourse, cunnilingus, fellatio or any other intrusions involving any part of a human body or of any object into the genital or anal opening of a person's body. In many countries rape and other sexual offences have been replaced with a series of gender neutral and graded offences with appropriate punishments. Medical examination can provide independent, scientific, corroborative evidence that may be of value to the court in arriving at a judgement. Doctors should have a clear understanding of different rape laws in order to apprectiate the various issues involved. Special knowledge, skill and experience are essential to conduct a good-quality medical examination. There is a dearth of trained forensic physicians in many Asian countries. However, managing a rape victim (survivor) goes for beyond proving the case in a court of law. There should be an adequate rehabilitation programme available to the victims to help them cope.

  15. Explaining wartime rape.

    Science.gov (United States)

    Gottschall, Jonathan

    2004-05-01

    In the years since the first reports of mass rapes in the Yugoslavian wars of secession and the genocidal massacres in Rwanda, feminist activists and scholars, human rights organizations, journalists, and social scientists have dedicated unprecedented efforts to document, explain, and seek solutions for the phenomenon of wartime rape. While contributors to this literature agree on much, there is no consensus on causal factors. This paper provides a brief overview of the literature on wartime rape in historical and ethnographical societies and a critical analysis of the four leading explanations for its root causes: the feminist theory, the cultural pathology theory, the strategic rape theory, and the biosocial theory. The paper concludes that the biosocial theory is the only one capable of bringing all the phenomena associated with wartime rape into a single explanatory context.

  16. Planting depth for oilseed calendula

    Science.gov (United States)

    Calendula (Calendula officinalis L.) is not only a popular ornamental plant in temperate climates, but also a potential oilseed crop. Its seed oil has high levels of calendic acid, which makes it a highly valued drying oil with important industrial applications. Much basic agronomic information on c...

  17. What Is Rape and Date Rape?

    Science.gov (United States)

    ... fault. What is sexual assault? Sexual assault and abuse is any kind of sexual activity that you do not agree to, including: inappropriate touching vaginal, anal, or oral sex sex that you say ‘no’ to rape ...

  18. Ukrainian cereals and oilseeds trade

    Directory of Open Access Journals (Sweden)

    Б. В. Духницький

    2016-07-01

    Full Text Available Purpose. To analyze peculiarities of Ukrainian cereals and oilseeds trade, the situation on the world market, and determine future prospects of its development. Methods. Analysis and synthesis, comparative evaluation, graphic procedure. Results. The role and place of Ukraine in a total grain supply to the world market was determined. Ukraine is a world’s top ten grain producer. Among domestic agricultural products, cereals, oilseeds and sunflower oil are in the highest demand in the world. In recent years, our state has reinforced its status as one of the leading exporters of cereals. The commodity pattern of cereals and oilseeds export was analyzed with specifying most in-demand positions and the main countries purchasing these pro­ducts. According to the results of 2015, Ukraine obtained the highest foreign currency revenue from export of corn, wheat and barley (in grain structure including soybeans and rapeseed (among oil crops. Key domestic and multinational operators are the main exporters of cereals and oilseeds in Ukraine and still hold their leading position. It was found a significant excess of import price of seeds as compared with export price of crops grown in Ukraine. Assortment of maize and sunflower seeds offered by major companies-producers in Ukraine was studied. Main trends of the world grain market development are considered. Conclusions. It was established that Ukraine is one of the major exporters of cereals and oilseeds. However, volatility of their prices significantly affects the export revenue that was decreasing even with increasing export quantities in kind. The dependence of domestic grain industry development on high-quality imported seed of maize and sunflower hybrids was recorded. It is expected that in the years to come Ukraine will maintain its strong positions in the world’s grain market.

  19. Experimental Study of Reciprocating Friction between Rape Stalk and Bionic Nonsmooth Surface Units

    Directory of Open Access Journals (Sweden)

    Zheng Ma

    2015-01-01

    Full Text Available Background. China is the largest producer of rape oilseed in the world; however, the mechanization level of rape harvest is relatively low, because rape materials easily adhere to the cleaning screens of combine harvesters, resulting in significant cleaning losses. Previous studies have shown that bionic nonsmooth surface cleaning screens restrain the adhesion of rape materials, but the underlying mechanisms remain unclear. Objective. The reciprocating friction between rape stalk and bionic nonsmooth metal surface was examined. Methods. The short-time Fourier transform method was used to discriminate the stable phase of friction signals and the stick-lag distance was defined to analyze the stable reciprocating friction in a phase diagram. Results. The reciprocating friction between rape stalk and metal surface is a typical stick-slip friction, and the bionic nonsmooth metal surfaces with concave or convex units reduced friction force with increasing reciprocating frequency. The results also showed that the stick-lag distance of convex surface increased with reciprocating frequency, which indicated that convex surface reduces friction force more efficiently. Conclusions. We suggest that bionic nonsmooth surface cleaning screens, especially with convex units, restrain the adhesion of rape materials more efficiently compared to the smooth surface cleaning screens.

  20. The Culture of Rape

    Science.gov (United States)

    2013-05-09

    h &Fine Arts/333-7723 lRRESPONSIBILITY d1 2. WILLIAM FAULKNER , DYSFUNCTIONAL FAMILY t) AND TIIE OLD SOUTH .. L 3. RAPE OF CHARLOTTE TEMPLE AND...Undergraduate Research Forum (CSURF). The presentations are titled respectively: REALITY OF VIOLENCE AS A PRODUCT OF IRRESPONSIBlllTY; WILLIAM ... FAULKNER , DYSFUNCTIONA.t FAMILY, AND THE OLD SOUTH; RAPE OF CHARLOTTE TEMPLE AND VICTIM-BLAMING; AMERICA, LINEARLY CYCLICAL Circle one: Abstract- Tech

  1. Older women: victims of rape.

    Science.gov (United States)

    Tyra, P A

    1993-05-01

    Older female rape victims usually live alone, are raped by strangers, experience physical force and injury, and also are robbed. Rape trauma syndrome, a nursing diagnosis, consists of an acute phase of disorganization, and a long-term phase of reorganization of the victim's lifestyle. Rape victims experience emotional, physical, and cognitive reactions to the trauma of rape. Nursing actions can include providing specific interventions to victims during the acute phase, identifying victims during routine exams, referring victims for ongoing counseling, conducting community education programs on primary prevention and available services, and participating in longitudinal rape studies.

  2. Rape Beyond Crime.

    Science.gov (United States)

    Kaplan, Margo

    2017-02-01

    Public health experts agree that sexual violence constitutes a significant public health issue. Yet criminal law dominates rape law almost completely, with public health law playing at best a small supporting role. Recent civil law developments, such as university disciplinary proceedings, similarly fixate on how best to find and penalize perpetrators. As a result, rape law continues to spin its wheels in the same arguments and obstacles. This Article argues that, without broader cultural changes, criminal law faces a double bind: rape laws will either be ineffective or neglect the importance of individual culpability. Public health law provides more promising terrain for rape prevention because it is a strong legal framework that can engage the complex causes of rape, including the social norms that promote sexual aggression. While criminal law can only punish bad behavior, public health interventions can use the more effective prevention strategy of promoting positive behaviors and relationships. They can also address the myriad sexual behaviors and social determinants that increase the risk of rape but are outside the scope of criminal law. Perhaps most importantly, public health law relies on evidence-based interventions and the expertise of public health authorities to ensure that laws and policies are effective. Transforming rape law in this way provides a framework for legal feminism to undertake the unmet challenge of "theorizing yes," that is, moving beyond how to protect women’s right to refuse sex and toward promoting and exploring positive models of sex. Criminal law is simply incapable of meeting this challenge because it concerns only what sex should not be. A public health framework can give the law a richer role in addressing the full spectrum of sexual attitudes and behaviors.

  3. The health condition of spring oilseed crops in relation to the fungi colonising their seeds

    Directory of Open Access Journals (Sweden)

    Barbara Majchrzak

    2013-12-01

    Full Text Available The research was conduced in the years 1999-2000. The aim of the research was to determine the health condition of overground parts and seeds of the following spring oilseed crops: crambe (Crambe abbysinica Hoechst. cv. B o r o w s k i, false flax (Camelina sativa L. cv. B o r o w s k a, spring rape (Brassica napus ssp. oleifera L. cv. M a r g o and oleiferous radish (Raphanus sativus var. oleiferus L. cv. P e g l e t t a. In all the years of the research alternaria blight was found on the leaves and siliques of spring rape and oleiferous radish and on the leaves and stems of crambe. False flax proved to be weakly infected by pathogens. On its leaves gray mould (Botrytis cinerea was found in all the years of the research. The disease was found on the siliques only in 1999. In 2000 powdery mildew was found on spring rape and false flax (respectively, Erysiphe crucifearum and E.cichoracearum. The weather conditions affected the intensity of the diseases on the studied spring oilseed crops. Alernaria genus, especially A.alternata was most commonly isolated from the seeds of examined plants. It constituted from 37% (in crambe to 63,3% (in spring rape of all the isolates. Of the remaining pathogenic species, numerous fungi of the Fusarium genus (F.avenaceum, F.culmorum, F.equiseti and F.oxysporum were isolated. They constituted from 1,0% (false flax to 17,3% (crambe of the isolates.

  4. Campus Gang Rape: Party Games?

    Science.gov (United States)

    Ehrhart, Julie K.; Sandler, Bernice R.

    The phenomenon of gang rape as it sometimes occurs on college campuses is described, with attention to causes, impacts on the victim and other students, responses the college should take, and prevention. Consideration is given to the role of alcohol, drugs, and pornography in fraternity gang rape; successful model programs for rape prevention…

  5. Salinity tolerance of germinating alternative oilseeds

    Science.gov (United States)

    Integrating oilseed crops into rotations can improve soil health benefits, nutrient retention, and pollinator provisions. Field margins represent areas where incorporation of oilseeds is feasible. However in the northern Great Plains, field margins can oftentimes be areas of saline soil, which can i...

  6. Rape (For Teens)

    Science.gov (United States)

    ... traces of a sexual assault. But being examined right away is the best way to ensure you get proper medical treatment. Immediate medical attention also helps when people decide to report the crime, providing evidence needed to prosecute the rapist if a criminal case is pursued. If you've been raped ...

  7. Precursors to rape: pressuring behaviors and rape proclivity.

    Science.gov (United States)

    Strain, Megan L; Hockett, Jericho M; Saucier, Donald A

    2015-01-01

    We developed measures assessing personal and normative attitudes toward two types of behaviors that are symptomatic of rape culture. We conceptualize sexual violence as existing on a continuum and argue that two types of behaviors may be potential antecedents to (and consequences of) sexual violence: attempts to pressure, which mimic the power dynamics of rape in a less aggressive fashion, and benevolent dating behaviors, which are accepted dating scripts in which men initiate action. We examined individuals' acceptance of these behaviors in relation to their attitudes toward rape victims and among men to rape proclivity. This initial work suggests that these constructs and measures may be useful to investigate in future research.

  8. The costs of rape.

    Science.gov (United States)

    Perilloux, Carin; Duntley, Joshua D; Buss, David M

    2012-10-01

    The current study examined costs experienced by victims of completed rape (n=49) and attempted sexual assault (n=91) using quantitative analyses of 13 domains: health, self-esteem, self-perceived attractiveness, self-perceived mate value, family relationships,work life, social life, social reputation, sexual reputation, desire to have sex, frequency of sex, enjoyment of sex, and long-term, committed relationships. Women also provided descriptive accounts of their experiences, and we used these to illustrate the costs in the victims' own words.Compared to victims of an attempted sexual assault, victims of a completed rape reported significantly more negative outcomes in 11 of the 13 domains. The most negatively affected domains were self-esteem, sexual reputation, frequency of sex, desire to have sex, and self-perceived mate value. Although victims of rape experienced more negative effects than victims of attempted sexual assault,both groups of victims reported negative effects in every domain.Discussion focuses on the implications of the differing degrees and patterns of the costs of attempted and completed sexual victimization.

  9. Mycotoxin production on rice, pulses and oilseeds

    Science.gov (United States)

    Begum, Fouzia; Samajpati, N.

    Mycotoxin-producing fungi were isolated from contaminated grains of rice, pulses and oilseeds sold in the local markets of Calcutta for human consumption. It was found that aflatoxin B1 was produced by Aspergillus flavus and Aspergillus parasiticus, aflatoxin G1 by A. flavus, ochratoxin by Aspergillus ochraceous, sterigmatocystin by Aspergillus japonicus and citrinin by Penicillium citrinum. Aflatoxin B1 (333-10416μg/kg) was produced by Aspergillus spp. in rice, pulses and oilseeds.

  10. Rape: Legal issues in mental health perspective.

    Science.gov (United States)

    Jiloha, R C

    2013-07-01

    Rape of women by men has occurred throughout recorded history and across cultures and religions. It is a crime against basic human right and a most common crime against women in India. In this article, rape is discussed from legal and mental health perspective. In India 'rape laws' began with enactment of Indian Penal Code in 1860. There have been subsequent amendments and the main issue of focus remained the definition of 'rape and inclusion of 'marital rape' in the ambit of rape. Law Commission Reports related to rape and the psychological impacts of rape have been discussed.

  11. Profile of rape victims attending the Karl Bremer Hospital Rape ...

    African Journals Online (AJOL)

    In addition, there is a lack of available data on the per-episode risk of HIV infection with specific sexual encounters,1 and in combination with poor ... the incident of sexual assault. • To obtain data regarding the use of newly provided ARV therapy as ..... Baleta A. Journalist's rape adds to rising rape statistics. Lancet 1999 ...

  12. Rape Myth Consistency and Gender Differences in Perceiving Rape Victims: A Meta-Analysis.

    Science.gov (United States)

    Hockett, Jericho M; Smith, Sara J; Klausing, Cathleen D; Saucier, Donald A

    2016-02-01

    An overview discusses feminist analyses of oppression, attitudes toward rape victims, and previously studied predictors of individuals' attitudes toward rape victims. To better understand such attitudes, this meta-analysis examines the moderating influences of various rape victim, perpetrator, and crime characteristics' rape myth consistency on gender differences in individuals' perceptions of rape victims (i.e., victim responsibility and blame attributions and rape minimizing attitudes). Consistent with feminist theoretical predictions, results indicated that, overall, men perceived rape victims more negatively than women did. However, this sex difference was moderated by the rape myth consistency within the rape vignettes. Implications for research are discussed. © The Author(s) 2015.

  13. Attitudes toward Rape and Victims of Rape: A Test of the Feminist Theory in Ghana

    Science.gov (United States)

    Boakye, Kofi E.

    2009-01-01

    This study explores the usefulness of the feminist theory in explaining attitudes toward rape and victims of rape in Ghana. The feminist theory of rape posits, inter alia, that patriarchy and gender inequality are major factors in the aetiology of rape and attitudes toward rape and that underlying patriarchy and gender inequality are gender…

  14. ASD and PTSD in Rape Victims

    Science.gov (United States)

    Elklit, Ask; Christiansen, Dorte M.

    2010-01-01

    In recent years, a number of studies have investigated the prediction of posttraumatic stress disorder (PTSD) through the presence of acute stress disorder (ASD). The predictive power of ASD on PTSD was examined in a population of 148 female rape victims who visited a center for rape victims shortly after the rape or attempted rape. The PTSD…

  15. A Synthesized (Biosocial) Theory of Rape.

    Science.gov (United States)

    Ellis, Lee

    1991-01-01

    Integrates features of contemporary theories of rape (feminist theory, social learning theory, evolutionary theory) with information on neurohormonal variables to formulate synthesized theory of rape. Synthesized theory of rape, consisting of four propositions, proposes that people are not all equally prone toward rape and that men are much more…

  16. RESEARCHES ON OILSEEDS MARKET IN ROMANIA

    Directory of Open Access Journals (Sweden)

    Elena SOARE

    2014-12-01

    Full Text Available This scientific paper presents the evolution of oilseeds market in Romania, during the period 2008-2013. In order to show as concise as possible the reality of the oilseeds sector, the research pictures, on the one hand, an evolution of oilseeds specific indicators and on the other hand, an evolution of external trade. Romania disposes of tradition and favourable pedo-climatic conditions for cultivating the oilseeds plants. This is demonstrated by the favourable results obtained in the last years, especially concerning the sunflower crop. In 2013, Romania registered a record for sunflower, occupying the first position in the European Union for cultivated area and production. A negative aspect is represented by the fact that our country has the best conditions in Europe for soybean crop, but it is still dependent on import. Romania imports a huge quantity of soybean oilcakes for animal feeding. Nowadays, Romania is an important actor in the sunflower world market, covering almost 19% of the global demand. In perspective, one could expect significant oilseeds productions with a positive influence on the agro-food trade balance in our country.

  17. Rape: Legal issues in mental health perspective

    Science.gov (United States)

    Jiloha, R. C.

    2013-01-01

    Rape of women by men has occurred throughout recorded history and across cultures and religions. It is a crime against basic human right and a most common crime against women in India. In this article, rape is discussed from legal and mental health perspective. In India ‘rape laws’ began with enactment of Indian Penal Code in 1860. There have been subsequent amendments and the main issue of focus remained the definition of ‘rape and inclusion of ‘marital rape’ in the ambit of rape. Law Commission Reports related to rape and the psychological impacts of rape have been discussed. PMID:24082245

  18. Rape trauma syndrome as scientific expert testimony.

    Science.gov (United States)

    Block, A P

    1990-08-01

    Behavioral science studies conducted on rape victims reveal a posttraumatic stress disorder which follows the attack known as rape trauma syndrome. Evidence of rape trauma syndrome can be very useful in explaining the behavior of rape victims. Rape trauma syndrome can help corroborate the victim's assertion of lack of consent and also help the jury understand the typical reactions of rape victims. Courts have held that expert testimony of rape trauma syndrome is admissible as evidence of (i) lack of consent, (ii) the amount of damages in civil suits, (iii) a defense to culpable behavior, and (iv) an explanation for behavior of the victim that is inconsistent with the claim of rape. Rape trauma syndrome meets the requirements for admissibility when it is used for the proper purpose and with adequate safeguards to prevent any unfair prejudice. Based on case precedent on the admissibility of rape trauma syndrome as scientific expert testimony, rape trauma syndrome should be admissible if (i) the evidence presented only shows the typical reactions to rape and does not make any legal conclusions as to whether the victim was raped, (ii) the expert is qualified, (iii) a proper foundation is laid, (iv) liberal cross-examination of the expert is allowed, and (v) the defense can introduce its own expert testimony on rape trauma syndrome.

  19. Tangent Curve Function Description of Mechanical Behaviour of Bulk Oilseeds: A Review

    Directory of Open Access Journals (Sweden)

    Sigalingging R.

    2015-01-01

    Full Text Available The application of tangent curve mathematical model for description of mechanical behaviour of selected bulk oilseeds, namely jatropha, sunflower, rape, garden pea, and common bean in linear compression was reviewed. Based on the review analysis, the tangent curve function has been developed using MathCAD 14 software which employs the Levenberg-Marquardt algorithm for data fitting optimal for tangent curve approximation. Linear compression parameters including force (N, deformation (mm, energy (J, and/or volume energy (J-m-3 can equally be determined by the tangent model. Additionally, the theoretical dependency between force and deformation characteristic curves can be defined by the force coefficient of mechanical behaviour (N and deformation coefficient of mechanical behaviour (mm-1 of the tangent model. In conclusion, the review results, however, shows that the tangent curve mathematical model which is dependent on experimental boundary conditions is potentialy useful for theoretical description of mechanical properties and deformation characteristics of bulk oilseeds in axial compression.

  20. An Application of Art Therapy to the Trauma of Rape.

    Science.gov (United States)

    Hargrave-Nykaza, Karen

    1994-01-01

    Explores rape in terms of specific trauma usually experienced after rape: posttraumatic stress disorder, rape trauma syndrome, and permanent life changes. Explores ways in which art therapy can help rape victim engage in healing. Reviews study of one rape victim and current literature as example of life-altering effects of rape and importance of…

  1. Rape reporting: "Classic Rape" and the behavior of law.

    Science.gov (United States)

    Clay-Warner, Jody; McMahon-Howard, Jennifer

    2009-01-01

    Two theories of rape reporting, the Classic Rape perspective and Black's Theory of the Behavior of Law, are tested in this article. We offer the first comprehensive multivariate test of Classic Rape predictions among a nationally representative sample of victims, as well as the first test of Black's predictions for rape reporting. Through the construction of multinomial regression models, we are able to examine reporting patterns for both victims and third parties. Weapon use and physical injury consistently predicted reporting. The likelihood of victim reporting significantly increased when assaults occurred either in public or through a "home blitz," whereas place of assault did not affect the likelihood of third-party reporting. On the other hand, victim-offender relationship significantly affected the likelihood of third-party reporting but was not significant in the victim-reporting models. There were mixed findings regarding Black's stratification and morphology predictions, and we found no significant effects for culture, organization, or social control. Overall, these findings lend greater support to the Classic Rape perspective than to Black's model.

  2. The impact of rape acknowledgment on survivor outcomes: The moderating effects of rape myth acceptance.

    Science.gov (United States)

    Wilson, Laura C; Newins, Amie R; White, Susan W

    2017-11-13

    Little is known about how rape acknowledgment relates to posttrauma functioning; recent research suggests the effect may depend on additional factors. In the current study, the moderating effect of rape myth acceptance (RMA) on the relationships between rape acknowledgment and mental health outcomes was examined. A sample of 181 female rape survivors recruited from a university completed an online survey assessing RMA, rape acknowledgment, depression symptoms, and alcohol use. Generally, the results supported that RMA moderated the influence of rape acknowledgment on depression symptoms and average quantity per drinking episode, but not frequency of alcohol use. The findings demonstrated that when individuals endorsed high levels of RMA, acknowledged rape survivors reported worse outcomes than unacknowledged rape survivors. Among individuals low on RMA, unacknowledged rape survivors reported worse outcomes than acknowledged rape survivors. It is recommended that clinicians recognize the role of survivor beliefs, such as RMA, in the relationship between labeling sexual assault experiences and mental health consequences. © 2017 Wiley Periodicals, Inc.

  3. Proposed Recruit Training Topic: Rape Awareness.

    Science.gov (United States)

    1984-03-01

    Washington, DC. Medea , A. and Thompson, K. Against Rape. New York: Farrar, Straus, and Giroud, 1974. Rape: A Preventative Inquiry (film) 345-10 DN...programmed text study guide. UNITED STATES COAST GUARD The Coast Guard Training Center, Cape May, New Jersey, does not include rape awareness/prevention...82. September 1982. Arlington, Virginia. Connell, N. and Wilson, C. Rape: The First Resource Book for Women. New York: New American Library, 1974

  4. Male Rape Victim and Perpetrator Blaming

    Science.gov (United States)

    Sleath, Emma; Bull, Ray

    2010-01-01

    One of four possible vignettes manipulated by (a) level of rape myth contained within them (low vs. high) and (b) type of rape (stranger vs. acquaintance) was presented to participants followed by scales measuring victim blame, perpetrator blame, belief in a just world, sex-role egalitarian beliefs, and male rape myth acceptance. Victim blaming…

  5. Rape and Women's Sexual Health in Nigeria

    African Journals Online (AJOL)

    chifaou.amzat

    Feminist theory is used to explain rape, the societal reaction to it and the health outcomes for the victims. The paper concludes that many health problems suffered by women in Nigeria are as a result of rape. Public health practitioners should devise mechanisms of eliciting rape information from victims so as to effectively ...

  6. Consequences for weed management in crop rotations by introducing imidazolinone-tolerant oilseed rape varieties

    OpenAIRE

    Krato, Christoph

    2012-01-01

    OSR (Brassica napus L.) is one of the most important arable oil crops globally and is grown on an area of 31,680,945 ha as winter- and spring-sown varieties. The harvest is mainly used in human nutrition, animal feeding and as a renewable resource for the production of paints, varnishes and biodiesel. OSR can be considered a quite competitive crop but nonetheless weed control is carried out on the vast majority of the grown area. The most common treatments are done PRE-E or early POST-E, m...

  7. Wild pollinators enhance oilseed rape yield in small-holder farming systems in China

    NARCIS (Netherlands)

    Zou, Yi; Xiao, Haijun; Bianchi, Felix J.J.A.; Jauker, Frank; Luo, Shudong; Werf, van der Wopke

    2017-01-01

    Background: Insect pollinators play an important role in crop pollination, but the relative contribution of wild pollinators and honey bees to pollination is currently under debate. There is virtually no information available on the strength of pollination services and the identity of pollination

  8. Plant defence responses in oilseed rape MINELESS plants after attack by the cabbage moth Mamestra brassicae.

    Science.gov (United States)

    Ahuja, Ishita; van Dam, Nicole Marie; Winge, Per; Trælnes, Marianne; Heydarova, Aysel; Rohloff, Jens; Langaas, Mette; Bones, Atle Magnar

    2015-02-01

    The Brassicaceae family is characterized by a unique defence mechanism known as the 'glucosinolate-myrosinase' system. When insect herbivores attack plant tissues, glucosinolates are hydrolysed by the enzyme myrosinase (EC 3.2.1.147) into a variety of degradation products, which can deter further herbivory. This process has been described as 'the mustard oil bomb'. Additionally, insect damage induces the production of glucosinolates, myrosinase, and other defences. Brassica napus seeds have been genetically modified to remove myrosinase-containing myrosin cells. These plants are termed MINELESS because they lack myrosin cells, the so-called toxic mustard oil mines. Here, we examined the interaction between B. napus wild-type and MINELESS plants and the larvae of the cabbage moth Mamestra brassicae. No-choice feeding experiments showed that M. brassicae larvae gained less weight and showed stunted growth when feeding on MINELESS plants compared to feeding on wild-type plants. M. brassicae feeding didn't affect myrosinase activity in MINELESS plants, but did reduce it in wild-type seedlings. M. brassicae feeding increased the levels of indol-3-yl-methyl, 1-methoxy-indol-3-yl-methyl, and total glucosinolates in both wild-type and MINELESS seedlings. M. brassicae feeding affected the levels of glucosinolate hydrolysis products in both wild-type and MINELESS plants. Transcriptional analysis showed that 494 and 159 genes were differentially regulated after M. brassicae feeding on wild-type and MINELESS seedlings, respectively. Taken together, the outcomes are very interesting in terms of analysing the role of myrosin cells and the glucosinolate-myrosinase defence system in response to a generalist cabbage moth, suggesting that similar studies with other generalist or specialist insect herbivores, including above- and below-ground herbivores, would be useful. © The Author 2015. Published by Oxford University Press on behalf of the Society for Experimental Biology.

  9. Indications of selenium protection against cadmium and lead toxicity in oilseed rape (Brassica napus)

    Science.gov (United States)

    Increased cadmium (Cd) and lead (Pb) pollution from industrial, agricultural, energy and municipal sources may have a toxic impact on food chain quality in China. In plants, Cd is readily taken up, while Pb is slowly absorbed. The uptake of both metals interferes with many cellular functions in the ...

  10. Coat protein of Turnip mosaic virus in oilseed rape (Brassica napus)

    African Journals Online (AJOL)

    mohammad

    Three host species, Chenopodium quinoa, Chenopodium amaranticolor and Brassica rapa were used in the inoculations, the first two hosts for biological purification and the last one for virus preservation. One of the infected sample from Govareshk of. Mashhad (IRN GSK) was ground in 100 mM potassium phosphate buffer ...

  11. Molecular characterization and identification of fungi causing stern canker of oilseed rape in Serbia

    Directory of Open Access Journals (Sweden)

    Mitrović Petar

    2017-01-01

    Full Text Available During 2008-2010 the infected Brassica napus plant material was collected from nine localities: Karavukovo, Crvenka, Prigrevica, Subotica, Rimski Šančevi, Srbobran, Beška, Banatsko Karađorđevo, and Srpski Miletić. Infected tissue samples were taken from the root of both upper and basal stems, as well as from the leaf, flower and pods. Two reference isolates from Great Britain were used in these studies for the analysis of 119 isolates from Serbia, using polymerase chain reaction (PCR and PCR-RFLP. Digestion of PCR products was performed with 5 selected endonucleases: BamHI, HaeIII, Rsal, EcoRII, and AluI. On the basis of PCR analysis, all isolates originating from Serbia belong to L. complex: 111 belong to species Leptosphaeria maculans, and 8 to species Leptosphaeria biglobosa NA1 ('Leptosphaeria biglobosa brassicae, respectively.

  12. Chitosan oligosaccharides-triggered innate immunity contributes to oilseed rape resistance against Sclerotinia sclerotiorum

    DEFF Research Database (Denmark)

    Yin, Heng; Yan, Li; HongYan, Zhang

    2013-01-01

    Chitosan oligosaccharides (collectively, oligochitosan, or COS) are considered to be potent plant immunity elicitors. In this article, the induction of resistance to Sclerotinia sclerotiorum in Brassica napus L. var. Huyou 15 by COS is studied. Even though COS (50 mg mL1) did not affect radial...

  13. The first occurrence of stem canker on oilseed rape caused by Leptosphaeria biglobosa in Serbia

    Directory of Open Access Journals (Sweden)

    Mitrović Petar

    2016-01-01

    Full Text Available In October 2010 the occurrence of the stem canker symptoms in rapeseed was observed at the locality Rimski Šančevi in Serbia. Several strains of fungi were isolated from the stem. Morphological characteristics of the isolates were studied on a PDA medium at 25±1oC: growth rate, colour, shape and appearance of colonies and the edge of the colony, the pigment, size, colour and shape of pycnidiospores and pycnidia in 10 isolates (isolated in Serbia K-111, IK-112, K-113, K-114, K-115, K-116, IK-117, K-118, K-119, and K-120. All tested strains had fast growth, the regular form of colonies and poor sporulation on nutrient medium. Pycnidiospores are unicellular, hyaline, and mostly straight, with or without a drop of oil. Molecular identification was performed by the application the PCR technique using primers PN3/PN10. In addition to these 10 isolates, two reference strains obtained from the Centre for Agricultural Studies, Rothamsted, UK, which are marked with L. m (Leptosphaena maculanS, L. b (Leptosphaeria biglobosa and 7 reference isolates originating from Serbia, which are marked K-7, St-16, GS-25, L-5, C-3, LJ-2, S-1 were used. On the basis of DNA amplification with primers PN3 and PN10 tape length was about 580 bp for isolates (L.b, IK-111, K-112, K-113, K-114, K-115, K-116, K-117, K-118, K-119, K-120 while that was 560 bp for other group of isolates (L.m, K-7, St-16, GS-25, L-5, C-3, LJ- 2, S-1.This study showed that 10 isolates (K-111, K-112, K-113, IK- 114, K-115, K-116, K-117, K-118, K-119, K-120 isolated from the stem of rapeseed belong to the species Leptosphaeria biglobosa.

  14. Reaction of winter oilseed rape varieties to elevated concentrations of lead

    Directory of Open Access Journals (Sweden)

    Oreščanin Bojana

    2012-01-01

    Full Text Available Remediation methods allow the removal of metals from contaminated soil, and phytoremediation a technology for cleaning contaminated soil and waste material by plants, is becoming increasingly used. Brassica napus L., as one of the main oilcrops and high-biomass producing species, is becoming more and more interesting for the use in phytoextraction as it is proved to be tolerant to higher concentrations of heavy metals. The aim of this study was to examine the specific responses of three commercial winter rapeseed varieties, Banaćanka, Slavica and Kata, to the increased concentrations of lead in vitro. Significant reduction in root length of plants treated with lead was observed only in the variety Slavica, indicating susceptibility of this variety to the increased concentrations of this heavy metal. As in variety Kata a significant reduction in the length of the above-ground part due to the treatment with lead was detected, it could be concluded that the variety Banaćanka is the most tolerant to the applied concentrations of lead since there were no significant changes in the growth and biomass accumulation in all treatments except one, and could be recommended for further use in phytoremediation studies. [Projekat Ministarstva nauke Republike Srbije, br. TR31025 i br. III43007

  15. Biologically based technologies for control of soil-borne plant pathogens of cucumber and oilseed rape

    Science.gov (United States)

    Sustainable intensification of food production is necessary if we are to feed the world’s future population and maintain the resources required to produce this food. Biologically based technologies for disease control, such as microbial biological control agents and cover crops, can be integral to ...

  16. The Effects of Weather on Oilseed Rape (OSR Yield in China: Future Implications of Climate Change

    Directory of Open Access Journals (Sweden)

    Yaqin He

    2017-03-01

    Full Text Available Understanding the role of climatic factors on crop yields is essential in predicting the future impact of climate change. In order to understand the influence of climatic factors on OSR, detailed farm-level panel data from 2566 farms across 67 counties of the 6 major OSR production regions in China, from the surveys conducted by the national OSR industry project between 2008 and 2013, were used to examine the contribution of changes in selected climatic variables between 2008 and 2013 to yield variation. Spatial and temporal patterns of the relationships between OSR yield, climatic factors were estimated together with the effects of farmer adaptation and management practices on yield variability. The analysis revealed that yields in the low-latitude production regions were more sensitive to temperature increases and likely to decline. Precipitation iwas the most influential factor on yield at the first two growth stages; temperature and sunshine hours were most important at the third and fourth growth stages, respectively. Labour input was the most influential management factor affecting yields compared with fertilizer and other inputs. The study concludes that projection of future climate change impacts will need inter alia to incorporate more sophisticated and detailed measures of climatic variables than simple means of temperature and precipitation, incorporating timing in relation to plant growth and yield.

  17. Response of oilseed Brassica cultivars to ozone

    Energy Technology Data Exchange (ETDEWEB)

    Hucl, P.; Beversdorf, W.D

    1993-10-01

    Research was conducted to determine the sensitivity of four Brassica oilseed crops to ozone under controlled environmental conditions. Cultivars of four Brassica oilseed species were exposed to acute levels of ozone (0.31 or 0.47 [mu]l/l) at the seedling stage. Brassica hirta BHL-926 was the most sensitive to ozone, followed by B. juncea (Newton) and B. rapa (Torch). Injury symptoms ranged from a slight stippling in insensitive species to large bifacial necrotic patches in the most sensitive species. Brassica napus (canola) seedlings exhibited very little foliar injury (0-4% after 24 h exposure to 0.31 [mu]l/l ozone). In Ontario, where a vast majority of canola acreage is seeded to B. napus cultivars, it is unlikely that canola yields are being affected by exposure to ozone. 6 refs., 1 tab.

  18. Characterization of Rape Field Microwave Emission and Implications to Surface Soil Moisture Retrievals

    Directory of Open Access Journals (Sweden)

    Alexander Loew

    2012-01-01

    Full Text Available In the course of Soil Moisture and Ocean Salinity (SMOS mission calibration and validation activities, a ground based L-band radiometer ELBARA II was situated at the test site Puch in Southern Germany in the Upper Danube Catchment. The experiment is described and the different data sets acquired are presented. The L-band microwave emission of the biosphere (L-MEB model that is also used in the SMOS L2 soil moisture algorithm is used to simulate the microwave emission of a winter oilseed rape field in Puch that was also observed by the radiometer. As there is a lack of a rape parameterization for L-MEB the SMOS default parameters for crops are used in a first step which does not lead to satisfying modeling results. Therefore, a new parameterization for L-MEB is developed that allows us to model the microwave emission of a winter oilseed rape field at the test site with better results. The soil moisture retrieval performance of the new parameterization is assessed in different retrieval configurations and the results are discussed. To allow satisfying results, the periods before and after winter have to be modeled with different parameter sets as the vegetation behavior is very different during these two development stages. With the new parameterization it is possible to retrieve soil moisture from multiangular brightness temperature data with a root mean squared error around 0.045–0.051 m³/m³ in a two parameter retrieval with soil moisture and roughness parameter Hr as free parameters.

  19. Why, when and how men rape: Understanding rape perpetration in ...

    African Journals Online (AJOL)

    This research confirms that rape is highly prevalent in South Africa, with only a small proportion of incidents reported to the police. Many of the roots of the problem lie in our accentuated gender hierarchy. This highlights the importance of interventions and policies that start in childhood and seek to change the way in which ...

  20. Cultural and legal accounts of rape

    Directory of Open Access Journals (Sweden)

    Fernández Villanueva, Concepción

    2008-11-01

    Full Text Available The present paper attempts to show how rape is represented in a variety of historical and contemporary discourses. Examples of rapes in Greek mythology, in the Bible, in medieval iconography, in literature, and in scientific theories, are compared and contrasted. Analysis suggests that such discourses tend to make sexual aggression (and specifically the rape of women legitimate, be it by minimizing the harm done; by normalizing and naturalizing sexual violence; or by aesthetisizing rape as art. Finally, the paper argues that putting modern legal accounts of rape into their historical perspective brings into sharper focus the persistence of ancient myths and discourses

  1. Marital rape: history, research, and practice.

    Science.gov (United States)

    Bennice, Jennifer A; Resick, Patricia A

    2003-07-01

    Despite the increased recognition that the topic of marital rape has generated in the past 2 decades, the literature in this area remains sparse. This article provides a comprehensive review of the current state of the marital rape literature. First, the lengthy history of legal, cultural, and professional invalidation of marital rape victims, and the resulting negative treatment implications, is discussed. Second, marital rape research is reviewed, including prevalence, descriptive, and comparison studies. This review highlights the seriousness of marital rape, in terms of prevalence and posttrauma distress, as well as the limitations of extant research. Finally, barriers to treatment and recommendations for professionals are discussed.

  2. Patterns of rape and approaches to care.

    Science.gov (United States)

    Pepiton-Rockwell, F

    1978-03-01

    The family physician is likely to be involved in the care of the rape victim. This paper focuses on the social and legal definitions of rape as well as the incidence of rape. The Rape Trauma Syndrome is explained and normative responses of victims are discussed. Eight common myths about rape are identified which the victim and the physician may share. In discussing the myths, guidelines for counseling the rape victim are delineated: encourage the victim to talk about her feelings; as the physician, remain nonjudgmental; provide information or resources to the victim; view the aftermath of rape as a response to a life-threatening event; and enlist the support of the victim's significant others. Finally, the importance of understanding the available community resources is stressed.

  3. Breaking the silence surrounding rape.

    Science.gov (United States)

    Ramsay, S

    1999-12-11

    This article documents the turning points in the rape issue, which led to the acknowledgement of its prevalence in the world, especially in South Africa. November 25 marked the first day of the "Take a Stand" movement in South Africa, which coincided with the International Day Against Violence Against Women. This movement involves peaceful protests and poster campaigns, followed by months of sustained news coverage of rape. This was launched by a coalition of organizations, including women's groups, churches, businesses, and trades unions, that are speaking out against all forms of sexual violence. In the international community, rape is starting to be taken seriously. In 1996, sexual assault was cited as a crime against humanity by the International Criminal Tribunal for the former Yugoslavia. In addition, a forum in London, UK, on November 25, discussed rape issues and recommendations on how to prevent such violence were given. The need for a change in legal systems, which currently discourage women from taking legal actions, was also recognized. In view of this, the medical community is challenged to provide sensitive and appropriate help to women who have been assaulted.

  4. Romeo, Juliet, and Statutory Rape.

    Science.gov (United States)

    Bierie, David M; Budd, Kristen M

    2016-07-15

    Statutory rape is an important yet understudied topic. There is broad public support for the prosecution of older adults who engage in sexual relationships with minors regardless of perceptions of consent by either party. However, some scholars worry that expansive definitions within these laws have led to the widespread involvement of the justice system in the lives of similarly aged teenagers engaging in relatively normal sexual behavior, so called "Romeo and Juliet" liaisons. This, in turn, has called into question the legitimacy of national policies, such as sex offender registration, because of the presumption that registries are likely filled with these kinds of cases which may not represent the intent of legislatures and the public. Despite the importance of these debates, there is little research assessing the prevalence of Romeo and Juliet cases in official crime statistics or that analyze differences in characteristics of statutory rape as a function of victim-offender age differences. Drawing on more than 20 years of police data from over 6,000 police departments in the United States, this study found statutory rape cases were rare and Romeo and Juliet cases were even rarer. Multivariate models showed several distinctions between statutory rape cases as a function of the age differences between victim and offender. Of note, the odds that additional forms of sexual aggression occurred in the incident grew as the age difference expanded. © The Author(s) 2016.

  5. The Geography of Rape: Rape Victims in Urban and Rural Communities

    DEFF Research Database (Denmark)

    Nielsen, Louise Hjort

    in Denmark. It was hypothesized that there would be a lower representation of rape victims, measured by the number of calls, to a regional rape crisis center in the rural communities further away from the regional rape crisis center of Aarhus compared to the urban communities closer to the rape crisis center....... A one-way ANOVA analysis showed that there was a significant association between the number of victim calls and the distance to the rape crisis center. The closer the residence of the victim was to the rape crisis center the more victim calls were registered. The study also examined what types of help...

  6. Radio-sensitivity analysis and selection of useful mutants of rape (Brassica napus L.) by gamma irradiation

    Energy Technology Data Exchange (ETDEWEB)

    Goh, Eun Jeong; Kim, Wook Jin; Kim, Jin Baek; Kim, Dong Sub; Kim, Sang Hoon; Kang, Si Yong [Korea Atomic Energy Research Institute, Jeongeup (Korea, Republic of)

    2010-09-15

    Rape (Brassica napus L.) plants are one of the major oilseed crops. The main components of rapeseed are oil (35 to 47%) and protein (15 to 32%). For the biodiesel production, the development of a new variety of rape plant with high biomass and/or oleic acid contents is required. In order to determine the optimum dose of gamma-ray irradiation, the rape seeds of cvs. Hanra (Hr), Youngsan (Ys), Tammi (Tm), and Tamra (Tr) were irradiated with a 100 {approx} 4,000 Gy dose range of gamma-rays. Considering the growth factors, the optimum doses were determined to be within the range of 600 {approx} 1,000 Gy for the selection of useful mutant lines. Six-hundred and eighty eight (688) M{sub 2} mutant lines were obtained from 600 {approx} 1,000 Gy gamma-ray-irradiated M{sub 1} plants through selfing. The growth characteristics, leaf shape, early flowering, and flower color were all investigated. The selected mutant numbers of early flowering, leaf shape, and flower color were 34, 52, and 3 from the four cultivars, respectively. These mutant lines will be used for the development of a new variety of rape plant with high biomass and oleic acid contents.

  7. Child rape: facets of a heinous crime.

    Science.gov (United States)

    Gangrade, K D; Sooryamoorthy, R; Renjini, D

    1995-01-01

    This article discusses the extent of child rape in India, case studies of girl children in legal procedures, rape settings and perpetrators, public morality, and the nature of rape laws in India. It is concluded that there is no safe place for children. Currently, rapists are allowed to go free or are acquitted. Prevention and control of child rape must involve punishment of rapists. It is not appropriate that society ostracize the victim and her family. Victims should not remain silent. National Crime Records Bureau statistics reveal increases in rape during 1986-91. State figures are given for 1986-88. Madhya Pradesh had the highest reported incidence of rape in 1988. In 1993, Madhya Pradesh had a total of 2459 rapes. Nationally, 10,425 women were reported as raped in 1991. 51.7% were 16-30 years old. There were 1099 cases of pedophilia in 1991, which was an increase over 1990. Over 50% of the pedophilia cases were reported in Uttar Pradesh. The record of convictions shows very low figures. 1992 trial results of 276 rape cases indicated that only 46 persons were convicted. Victims suffer from psychological effects of embarrassment, disgust, depression, guilt, and even suicidal tendencies. There is police and prosecution indifference as well as social stigma and social ostracism of the victim and her family. Many cases go unreported. The case studies illustrate the difficulties for the victim of experiencing the rape and the social responses: police harassment, shame and fear, and occasionally public outrage. The case studies illustrate rape in familiar settings, such as schools, family homes, and neighbors and friends' homes; rape by policemen; and rape by political influentials. Most offenders are young, married, and socioeconomically poor. Mass media portrayals fuel the frustrations of poor and lonely men in cities. Rapists exhibit anti-social behavior or psychopathology. Sexual offenses are related to society's moral values.

  8. War rape, natality and genocide.

    Science.gov (United States)

    Schott, Robin May

    2011-01-01

    Feminist philosophy can make an important contribution to the field of genocide studies, and issues relating to gender and war are gaining new attention. In this article I trace legal and philosophical analyses of sexual violence against women in war. I analyze the strengths and limitations of the concept of social death—introduced into this field by Claudia Card—for understanding the genocidal features of war rape, and draw on the work of Hannah Arendt to understand the central harm of genocide as an assault on natality. The threat to natality posed by the harms of rape, forced pregnancy and forced maternity lie in the potential expulsion from the public world of certain groups—including women who are victims, members of the 'enemy' group, and children born of forced birth.

  9. Rape and the Serial Rapist

    Science.gov (United States)

    1993-12-01

    suffered from various personality disorders . "Twenty-two (16 percent) were diagnosed as borderline personalities , and thirteen (10 percent) showed...75 percent) were predominantly diagnosed as suffering from a personality disorder . Richard T. Rada in his study found that the category of the...Rape: The Psychology of the Offender (New York: Plenum Press), 109. 45 Fritz A. Kenn et al., " Forensics Psychiatry: Profiles of Two Types of Sex

  10. Rape: psychopathology, theory and treatment.

    Science.gov (United States)

    Gannon, Theresa A; Collie, Rachael M; Ward, Tony; Thakker, Jo

    2008-07-01

    Whether treatment programs are effective at rehabilitating rapists is yet to be determined empirically. From a scientist-practitioner perspective, treatment should be based on an empirical understanding of rape and rapists, and evidence-based knowledge of treatment outcome with rapists. In this paper we comprehensively review the characteristics of rapists, etiological features implicated in the commission of rape, and relevant treatment outcome research. We pay particular attention to contemporary knowledge about the core vulnerabilities and features required to understand and treat rapists effectively, and, where possible, highlight similarities and differences between rapists, child molesters and non-sexual violent offenders. We use an epistemological framework to (a) critique the various etiological accounts of rape available and (b) help guide professionals' use of such knowledge in both treatment design and evaluation. Gaps in the understanding of rapists' characteristics and etiological features are highlighted, as are discrepancies between current knowledge and treatment approaches. We conclude by highlighting areas for future research and practice innovation.

  11. Response of winter rape (Brassica napus L. ssp. oleifera Metzg., Sinsk to foliar fertilization and different seeding rates

    Directory of Open Access Journals (Sweden)

    Cezary A. Kwiatkowski

    2012-12-01

    Full Text Available A field experiment in growing winter rape was carried out during the period 2009-2011 in a family farm (owned by Mr. M. Bednarczyk located in Jaroszewice (Lublin region, on podzolic soil. Plant biometric features as well as yield and seed qualitative parameters (oil, protein and glucosinolate content were evaluated depending on the following rates of soil NPK fertilizers and on foliar fertilization (autumn spraying with the fertilizer solution: 100% and 75% of NPK as well as urea + nickel chelate + MgSO4H2O; 100% and 75% of NPK as well as urea + Plonvit R + MgSO4H2O. Plots without foliar fertilization (only 100% of NPK were the control treatment. The other experimental factor was the seeding rate (2.5 kg×ha-1 – 30 cm row spacing; 4 kg×ha-1 – 18 cm row spacing. Foliar spraying was done once in the autumn in the second decade of October. Tillage as well as mechanical and chemical control of agricultural pests in the plantation were typical for this plant species and consistent with the recommendations for winter rape protection. A hypothesis was made that the application of foliar fertilizers would have a beneficial effect on winter rape productivity, at the same time maintaining the high quality of raw material. It was also assumed that a reduction in the seeding rate of winter oilseed rape would result in reduced plant lodging and an increased number of siliques per plant; as a consequence, seed and oil productivity would be at a level not lower than that obtained at the higher seeding rate. The present study has proved that foliar fertilization of winter oilseed rape in the autumn period contributes to improved plant winter hardiness and increased productivity. The application of foliar fertilizers also enables the rates of basic mineral NPK fertilizers to be reduced by 25% without detriment to seed yield. Foliar fertilizers have been found to have a weaker effect on changing the chemical composition of rapeseed. The study has shown that

  12. Fear of rape from behind prison walls.

    Science.gov (United States)

    Shermer, Lauren O'Neill; Sudo, Heather

    2017-06-12

    Purpose The Prison Rape Elimination Act has brought significant attention to the issue of sexual victimization within correctional institutions. While the actual risk of sexual victimization remains low, the perception of rape among inmates is high. Given how one's fear can translate into behavior, understanding how institutions impact the culture surrounding prison rape highlights areas for reducing violence within prisons. The paper aims to discuss these issues. Design/methodology/approach This study includes secondary analysis of a quantitative database created from semi-structured interviews with 564 high security, general population inmates. Using fear of rape as the outcome of interest, bivariate and logistic regression analyses are used to comment on the impact of individual and facility level characteristics on this outcome. Findings In general, the results from this study suggest that the greatest risk factors for fearing rape while in prison are being male, having a mental health issue, and hearing about rape within the institution. From these specific findings a few general lessons can be learned with the hope that practitioners can translate these lessons into policy initiatives in order to combat fear of rape among our inmate population. Originality/value This paper aims to fill a gap in the research on how the facility contributes to the fear of rape within prison. The end goal is to inform policy makers so that suggestions can be made to combat this problem and prevent further misconduct within these facilities.

  13. Japanese International Students' Attitudes toward Acquaintance Rape.

    Science.gov (United States)

    Motoike, Janice; Stockdale, Margaret

    This study looked at the influence of an Asian sociocultural variable, loss of face or social integrity, as a predictor of perceptions of acquaintance rape among Japanese students. In addition to the expected associations between gender, sexism, and perceptions of rape, loss of face was predicted to interact with the perpetrator's reference group…

  14. Rape victim assessment: Findings by psychiatrists and ...

    African Journals Online (AJOL)

    valid at the lower end of the IQ range. The assessment of rape victims is a sensitive matter and poses a number of challenges to the clinician. Rape victims are often traumatised by their experience, and this can make them reluctant to talk about the incident. In a study done by Elklit et al.,[2] it was found that ~70% of sexual ...

  15. Psychobiological correlates of rape in female adolescents

    NARCIS (Netherlands)

    Bicanic, I.A.E.

    2014-01-01

    A considerable percentage of youth in the Netherlands has been victimized by rape during adolescence. The present research shows that adolescence rape is correlated with high levels of psychological distress, neurobiological dysregulations and increased risk for problems with sexual and pelvic floor

  16. Mycoflora and mycotoxin production in oilseed cakes during farm storage.

    Science.gov (United States)

    Lanier, Caroline; Heutte, Natacha; Richard, Estelle; Bouchart, Valerie; Lebailly, Pierre; Garon, David

    2009-02-25

    Agricultural activities involve the use of oilseed cakes as a source of proteins for livestock. Because the storage of oilseed cakes could induce the development of molds and the production of mycotoxins, a survey was conducted during the 5 months of farm storage. Mycoflora was studied by microscopic examinations, and the presence of Aspergillus fumigatus was confirmed by polymerase chain reaction. A multimycotoxin method was developed to quantify seven mycotoxins (aflatoxin B(1), alternariol, fumonisin B(1), gliotoxin, ochratoxin A, T-2 toxin, and zearalenone) in oilseed cakes by high-performance liquid chromatography coupled to mass spectrometry. Among 34 fungal species identified, A. fumigatus and Aspergillus repens were observed during 5 and 4 months, respectively. Gliotoxin, an immunosuppressive mycotoxin, was quantified in oilseed cakes up to 45 microg/kg, which was associated with the presence of toxigenic isolates of A. fumigatus.

  17. The globalization of international oilseeds trade

    Directory of Open Access Journals (Sweden)

    Mittaine Jean-François

    2012-09-01

    Full Text Available In a globalised world, the trade of goods and services constitute the heart of the economic system. But the trade patterns may show extreme diversity depending upon the nature of the concerned products. The technical and functional constraints do impose their laws, particularly when they relate to biological products/commodities. The term globalization is generally used to describe a complex process of international integration. When referring to oilseeds, the term must also relate to agriculture and human nutritional requirements for a rising human population spread all over the world, particularly in large urban environment. With an annual harvest of about 450 Mn T, oilseeds are naturally “globalized” in their production patterns. The complexity of their production schemes associated with the diversity of their usage on widespread geographical regions gives this group of commodities a unique role on the world markets. This process of opened trade channels has enabled the sector to meet the supply requirements of a quickly rising demand coming from both the population and all the industrial usages including alternative “green” energy. However, for the time being, demand growth has not been fully counter-balanced by production growth. As a consequence, unless some strict reallocation of acreage is effectively organized or new high yielding technologies are developed, it is a fact that vegetable oil will keep a strong demand base for a long time, compounded by the mechanical demand from economic growth in highly populated emerging economies. The balancing of supply and demand is expected to remain a tight exercise every year. At the end of the day, the future of the entire industry largely lies in technology because the final stake is to ensure the proper feeding of a 9 billion people population in a not too far future.

  18. Negligent Rape and Reasonable Beliefs

    DEFF Research Database (Denmark)

    Hansen, Pelle Guldborg

    2008-01-01

    In every known society human sexual intercourse is enacted as a rule-bound practice that involves certain rights and obligations. In Denmark the official policy is that the rules to govern lawful sexual intercourse should be based on the idea of mutual and voluntary consent between two adults......; and as a matter of fact such rules are accepted and followed by far the most. Yet, the approach taken by Danish Criminal Law to rape does not pay heed to these rules, but is rather based on a most narrow conception of male responsibility and obligation that stands in clear opposition with practice and policy...

  19. Present and future instances of virtual rape in light of three categories of legal philosophical theories on rape

    NARCIS (Netherlands)

    Strikwerda, Litska

    2015-01-01

    This paper is about the question of whether or not virtual rape should be considered a crime under current law. A virtual rape is the rape of an avatar (a person’s virtual representation) in a virtual world. In the future, possibilities for virtual rape of a person him- or herself will arise in

  20. Male rape--the silent victims.

    Science.gov (United States)

    Ellis, Colin Derek

    2002-10-01

    This literature review examines the issue of male rape and the possible counselling strategies that may assist in recovery. There are several misconceptions surrounding male rape which can result in the under-reporting and secondary or sanctuary victimisation of the survivor. Men who have been raped may believe that it attacks the very essence of what it is to be masculine and male. Many may not seek help unless they perceive a need for immediate attention, such as physical trauma requiring medical assistance. The literature also reveals that when men do seek help they may be treated poorly. Secondary victimisation or sanctuary trauma results when there is a lack of empathy and understanding of the effect that rape can have on the survivor, such as rape-trauma syndrome. Training in this area is needed for the police, emergency department staff, nurses, general practitioners, and community health services. Management of survivors starts with examining our own beliefs about male rape. Many of the reported counselling strategies are based on therapists' observations, trauma theories, and research related to child abuse or sexual assault of females which may not be transferable to men who have been raped. Education and counselling of survivors' support networks must be considered as part of a holistic approach to management. Secondary prevention strategies aimed at the broader community are required to assist survivors to come forward without fear of further victimisation because of stereotyping.

  1. Spousal rape: A challenge for pastoral counsellors

    Directory of Open Access Journals (Sweden)

    James A. Glanville

    2013-01-01

    Full Text Available This article reflects on the criticism regarding the pastoral counsellor’s dealings with spousal rape victims. It argues that counsellors should be sensitive not to be biased, either personally or theologically, and should have an understanding of the biopsychosocial (biological, psychological and social impact of spousal rape, such as rape-related post-traumatic stress and other related illnesses such as depression, victimisation and stigmatisation. The pastoral counsellors should be aware of the legal and medical ramifications of spousal rape and have knowledge of the correct referral resources and procedures (trusted professionals, shelters and support structures. They should be self-aware and understand the effect that gender or previous traumatic personal experiences may have on their reactions. The article consists of the following sections: the phenomenon ‘rape’; acquaintance rape; spousal rape; post-traumatic stress; post-traumatic stress disorder; rape trauma syndrome; cognitive behavioural therapy; spirituality; doctrinal matters; social system of patriarchy; a pastoral counselling model; self-care.

  2. Understanding Attribution of Blame in Cases of Rape: An Analysis of Participant Gender, Type of Rape and Perceived Similarity to the Victim

    Science.gov (United States)

    Grubb, Amy Rose; Harrower, Julie

    2009-01-01

    This study examined a variety of factors that may influence attributions towards rape victims. A total of 156 participants completed a questionnaire, which included a measure of attitudes towards rape victims and a vignette depicting one of three rape scenarios (a stranger rape, date rape and seduction rape). Participants rated the extent to which…

  3. Institutional Perpetuation of Rape Culture: A Case Study of the University of Colorado Football Rape and Recruiting Scandal

    OpenAIRE

    Folchert, Kristi Engle

    2008-01-01

    Heterosexual rape among university students is neither new nor uncommon. Research in rape prevention is largely limited to two areas: (1) what women can do to protect themselves from rape, which continues to place the burden of prevention on women; and (2) understanding why men rape. The studies in these fields have added to the body of knowledge surrounding rape on university campuses. The current study takes this information into consideration while using a different lens to examine the...

  4. What is a typical rape? Effects of victim and participant gender in female and male rape perception.

    Science.gov (United States)

    Anderson, Irina

    2007-03-01

    The study had three research aims: (1) to examine the current perception of female rape. Given recent changes in public awareness of female rape, it was predicted that respondents would conceptualize a typical female rape as an acquaintance rape rather than as the stranger rape stereotype; (2) to examine whether these perceptions differ according to respondents' gender; (3) to examine the 'cultural lag' theory of male rape, where it was hypothesized that if the public perception of male rape lags behind female rape, then a typical male rape will be conceptualized as the classic stranger rape stereotype. Findings showed that contrary to predictions, a typical female rape was conceptualized according to the stranger rape stereotype. It was also found that instead of lagging behind female rape along the stranger-acquaintance rape dimension, male rape was viewed predominantly in terms of 'other' factors (factors not found on the stranger-acquaintance dimension, e.g. victim/rapist sexual orientation, rapist calls victim names), which were erroneous, sexualizing and homophobic.

  5. ASD and PTSD in Rape Victims

    DEFF Research Database (Denmark)

    Elklit, Ask; Christiansen, Dorte M

    2010-01-01

    In recent years, a number of studies have investigated the prediction of posttraumatic stress disorder (PTSD) through the presence of acute stress disorder (ASD). The predictive power of ASD on PTSD was examined in a population of 148 female rape victims who visited a center for rape victims...... shortly after the rape or attempted rape. The PTSD diagnosis based solely on the three core symptom clusters was best identified by a subclinical ASD diagnosis based on all ASD criteria except dissociation. However, a full PTSD diagnosis including the A2 and F criteria was best identified by classifying...... victims according to a full ASD diagnosis. Regardless of whether cases were classified according to full PTSD status or according to meeting the criteria for the three PTSD core symptom clusters, the classification was correct only in approximately two thirds of the cases. A regression analysis based...

  6. Understanding rape perpetration in South Africa

    African Journals Online (AJOL)

    a child. <15. Raped in the last. 12 months. Raped a man. SA Crime Quarterly no 34 • December 2010. 25 woman or girl when she didn't consent to sex or after you .... Secondary schooling. 26.7. 22.7, 30.7. 182 / 682. Matric complete. 28.9. 24.2, 33.5. 130/ 450. Incomplete tertiary education. 38.8. 30.4, 47.2. 57 / 147. Degree.

  7. A review on the challenges for the increased production of oilseeds ...

    African Journals Online (AJOL)

    A review on the challenges for the increased production of oilseeds in Ethiopia. ... Ethiopian Journal of Agricultural Sciences ... This paper presents the trends of production, utilization and export of oilseeds and import of vegetable oils; available technologies and the prospects of increasing oilseeds production in Ethiopia.

  8. Oilseeds for use in biodiesel and drop-in renewable jet fuel

    Science.gov (United States)

    Oilseeds, primarily soybean and canola, are currently used as feedstocks for biodiesel production. Oilseeds can also be used to produce drop-in renewable jet fuel and diesel products. While soybean and canola are the most common oilseed crops used for renewable fuel production in the U.S., many othe...

  9. The Effect of Biodiesel Policies on World Oilseed Markets and Developing Countries

    OpenAIRE

    Drabik, Dusan; de Gorter, Harry; Timilsina, Govinda R.

    2013-01-01

    Using an empirical model, this study provides some insights into the functioning of the oilseed-biodiesel-diesel market complex in a large country that determines the biodiesel price, reflecting market equilibrium changes resulting from volatility in the crude oil price. Oilseed crushing produces joint products -- oil and meal -- and this weakens the link between the biodiesel and oilseed...

  10. Taking About Rape-and Why it Matters: Adjudicating rape in the ...

    African Journals Online (AJOL)

    ... begins by establishing why judgements are an important source for understanding what rape means in society at large; it then discusses the relationship between power, language, and the law. This is followed by specific analyses of cases that show how patriarchy still defines how judges express themselves about rape.

  11. Effect of water stress on the agressiveness of oilsseed rape (Brassica napus L.) and two mustards (Sinapis alba L. and S. arvensis L.).

    Science.gov (United States)

    Maataoui, A; Talouizte, A; Benbella, M; Bouhache, M

    2003-01-01

    Oilseed rape (Brassica napus L.), a winter sown crop, may compete for water especially with Brassicaceae weeds. Investigating plant competition under water stress conditions is necessary for achieving a good yield in a Mediterranean climate characterized by a scarse water availability. This experiment was carried out to study the competiveness of oilseed rape (Brassica napus L.) with two brassicaceae weeds (Sinapis alba L. and S. arvensis L.). Species were grown at a density of two plants per bucket either in monoculture or as a binary mixture under water stress conditions in a greenhouse. Results of monoculture showed that B. napus had the highest shoot dry matter. Shoot dry matter of B. napus was more reduced by intraspecific competition than by interspecific competition due to S. arvensis. Shoot dry matter of S. alba in monoculture was higher than in mixture with S. arvensis, but more reduced in mixture with B. napus. In case of S. arvensis, shoot dry matter was more reduced by interspecific competition than by intraspecific competition. Agressivity based on grain yield showed, that B. napus was the most agressive species followed by S. alba. This agressivity did not change by the imposed water stress.

  12. The Effects of Rape Residue Mulching on Net Global Warming Potential and Greenhouse Gas Intensity from No-Tillage Paddy Fields

    Science.gov (United States)

    Zhang, Zhi-Sheng; Cao, Cou-Gui; Guo, Li-Jin; Li, Cheng-Fang

    2014-01-01

    A field experiment was conducted to provide a complete greenhouse gas (GHG) accounting for global warming potential (GWP), net GWP, and greenhouse gas intensity (GHGI) from no-tillage (NT) paddy fields with different amounts of oilseed rape residue mulch (0, 3000, 4000, and 6000 kg dry matter (DM) ha−1) during a rice-growing season after 3 years of oilseed rape-rice cultivation. Residue mulching treatments showed significantly more organic carbon (C) density for the 0–20 cm soil layer at harvesting than no residue treatment. During a rice-growing season, residue mulching treatments sequestered significantly more organic C from 687 kg C ha−1 season−1 to 1654 kg C ha−1 season−1 than no residue treatment. Residue mulching significantly increased emissions of CO2 and N2O but decreased CH4 emissions. Residue mulching treatments significantly increased GWP by 9–30% but significantly decreased net GWP by 33–71% and GHGI by 35–72% relative to no residue treatment. These results suggest that agricultural economic viability and GHG mitigation can be achieved simultaneously by residue mulching on NT paddy fields in central China. PMID:25140329

  13. Exploring rape myths, gendered norms, group processing, and the social context of rape among college women: a qualitative analysis.

    Science.gov (United States)

    Deming, Michelle E; Covan, Eleanor Krassen; Swan, Suzanne C; Billings, Deborah L

    2013-04-01

    The purpose of this research is to explore the negotiation strategies of college women as they interpret ambiguous rape scenarios. In focus groups, 1st- and 4th-year college women were presented with a series of three vignettes depicting incidents that meet the legal criteria for rape yet are ambiguous due to the presence of cultural rape myths, contexts involving alcohol consumption, varying degrees of consent, and a known perpetrator. These contexts are critical in understanding how college women define rape. Key findings indicated many of these college women utilized rape myths and norms within their peer groups to interpret rape scenarios.

  14. A REVIEW OF LITERATURE: RAPE AND COMMUNICATION MEDIA ...

    African Journals Online (AJOL)

    A REVIEW OF LITERATURE: RAPE AND COMMUNICATION MEDIA. STRATEGIES IN NIGERIA. *Omolola Tosan AKINWOLE. **Osakue Stevenson OMOERA. Abstract. Using historical-analytic methodology, this article reviews literature on rape and the ... The third assumption is the linguistic representation postulation. This.

  15. Religious Affiliation, Religiosity, Gender, and Rape Myth Acceptance: Feminist Theory and Rape Culture.

    Science.gov (United States)

    Barnett, Michael D; Sligar, Kylie B; Wang, Chiachih D C

    2016-08-24

    Rape myths are false beliefs about rape, rape victims, and rapists, often prejudicial and stereotypical. Guided by feminist theory and available empirical research, this study aimed to examine the influences of gender, religious affiliation, and religiosity on rape myth acceptance of U.S. emerging adults. A sample of 653 university students aged 18 to 30 years were recruited from a large public university in the southern United States to complete the research questionnaires. Results indicated that individuals who identified as Roman Catholic or Protestant endorsed higher levels of rape myth acceptance than their atheist or agnostic counterparts. Men were found more likely to ascribe to rape myths than their female counterparts. Religiosity was positively associated with rape myth acceptance, even after controlling the effect of conservative political ideology. No significant interaction was found between gender and religious affiliation or gender and religiosity. Limitations, future research directions, and implications of the findings are discussed from the perspective of feminist theory. © The Author(s) 2016.

  16. Green solvents and technologies for oil extraction from oilseeds.

    Science.gov (United States)

    Kumar, S P Jeevan; Prasad, S Rajendra; Banerjee, Rintu; Agarwal, Dinesh K; Kulkarni, Kalyani S; Ramesh, K V

    2017-01-01

    Oilseeds are crucial for the nutritional security of the global population. The conventional technology used for oil extraction from oilseeds is by solvent extraction. In solvent extraction, n-hexane is used as a solvent for its attributes such as simple recovery, non-polar nature, low latent heat of vaporization (330 kJ/kg) and high selectivity to solvents. However, usage of hexane as a solvent has lead to several repercussions such as air pollution, toxicity and harmfulness that prompted to look for alternative options. To circumvent the problem, green solvents could be a promising approach to replace solvent extraction. In this review, green solvents and technology like aqueous assisted enzyme extraction are better solution for oil extraction from oilseeds. Enzyme mediated extraction is eco-friendly, can obtain higher yields, cost-effective and aids in obtaining co-products without any damage. Enzyme technology has great potential for oil extraction in oilseed industry. Similarly, green solvents such as terpenes and ionic liquids have tremendous solvent properties that enable to extract the oil in eco-friendly manner. These green solvents and technologies are considered green owing to the attributes of energy reduction, eco-friendliness, non-toxicity and non-harmfulness. Hence, the review is mainly focussed on the prospects and challenges of green solvents and technology as the best option to replace the conventional methods without compromising the quality of the extracted products.

  17. Flowering dynamics and pollinator visitation of oilseed echium (Echium plantagineum)

    Science.gov (United States)

    Echium (Echium plantagineum L.) is an alternative oilseed crop in summer-wet temperate regions that provides floral resources to pollinators. Its seed oil is rich in omega-3 fatty acids, such as stearidonic acid, which is desired highly by the cosmetic industry. We examined flowering dynamics, polli...

  18. The global market for oilseeds: prospects and challenges for Morocco

    Directory of Open Access Journals (Sweden)

    Gosselet Nathalie

    2014-03-01

    Full Text Available The global market for oilseeds, grains, vegetable oil and oilseed meal is a complex market which is growing at a very fast pace, and which is characterized by the large volume of production which is traded between countries. Offer is geographically constrained in this market: there are few exporting countries and these are mainly situated on the American continent. Demand on the other hand is more widespread, although highest in Asiatic countries, China in particular. As a result, small importing countries, like Morocco, are in a vulnerable position, and take the full brunt of price volatility. In the 90s, Moroccan oilseed production was relatively high, unfortunately production dropped over the years, and Morocco must now buy vegetable oil and proteins on the global market. Reviving oilseed production in Morocco would considerably help the country and provide numerous benefits, such as food security, improving the country’s trade balance, and enhancing the agronomic management of land thanks to the introduction of break crops. Finally, it would also boost the entire agricultural sector and help increase the income of farmers.

  19. Sesame: the Underexploited Organic Oilseed Crop | Olowe | Journal ...

    African Journals Online (AJOL)

    Sesame (Sesamum indicum L.) is an important oilseed crop that ranks sixth among vegetable oils worldwide. Asia and Africa respectively account for 2.55 and 0.95 of the 3.66 million tons produced worldwide. However, Africa's net export of the commodity is just 38% of its production, despite the fact that the Continent has ...

  20. Rape survivor care crisis – mines the worst?

    African Journals Online (AJOL)

    The dire need for better healthcare worker training in rape forensics and safe survivor care has never been better illustrated than by the horrifying rape statistics Médecins. Sans Frontières (MSF) presented last month from the platinum-mining boom town of. Rustenburg. Promising to increase its rape survivor care and ...

  1. Exposure to Pornography and Acceptance of Rape Myths.

    Science.gov (United States)

    Allen, Mike; And Others

    1995-01-01

    Summarizes the literature examining the association between acceptance of rape myths and exposure to pornography. States that nonexperimental methodology shows that exposure to pornography does not increase rape myth acceptance, while experimental studies show that exposure to pornography increases rape myth acceptance. Concludes that experimental…

  2. Working Together To Change the Rape and Violence Culture.

    Science.gov (United States)

    Abrams, Julie M.; And Others

    This publication is a collection of 20 supporting documents for a conference program, "Working Together To Change the Rape and Violence Culture." Contents include: (1) "Presenter Contact Information"; (2) "Characteristics of Rape-Prone versus Rape-Free Cultures"; (3) "Dater's Bill of Rights"; (4)…

  3. 77 FR 30871 - Implementing the Prison Rape Elimination Act

    Science.gov (United States)

    2012-05-23

    ... May 23, 2012 Part VI The President Memorandum of May 17, 2012--Implementing the Prison Rape... 3-- #0;The President ] Memorandum of May 17, 2012 Implementing the Prison Rape Elimination Act... assault on human dignity and an affront to American values. The Prison Rape Elimination Act of 2003 (PREA...

  4. 42 CFR 50.306 - Rape and incest.

    Science.gov (United States)

    2010-10-01

    ... 42 Public Health 1 2010-10-01 2010-10-01 false Rape and incest. 50.306 Section 50.306 Public... § 50.306 Rape and incest. Federal financial participation is available in expenditures for medical procedures performed upon a victim of rape or incest if the program or project has received signed...

  5. Rape Myth Acceptance, Sexual Trauma History, and Posttraumatic Stress Disorder

    Science.gov (United States)

    Baugher, Shannon N.; Elhai, Jon D.; Monroe, James R.; Gray, Matt J.

    2010-01-01

    The prediction of false rape-related beliefs (rape myth acceptance [RMA]) was examined using the Illinois Rape Myth Acceptance Scale (Payne, Lonsway, & Fitzgerald, 1999) among a nonclinical sample of 258 male and female college students. Predictor variables included measures of attitudes toward women, gender role identity (GRI), sexual trauma…

  6. Theoretical Consideration of Forcible Rape: A Critical Analysis.

    Science.gov (United States)

    Clagett, Arthur F.

    1988-01-01

    Examined differences in hypothetical apperceptive fantasies of committing forcible rape, which are held by male subjects, as compared with the hypothetical apperceptive fantasies of being forcibly raped, held by the female subjects. Developed a critical analysis of social and cross-cultural variables affecting rape. (Author/ABL)

  7. An Empirical Investigation of Campus Demographics and Reported Rapes

    Science.gov (United States)

    Wiersma-Mosley, Jacquelyn D.; Jozkowski, Kristen N.; Martinez, Taylor

    2017-01-01

    Objective: Rape on college campuses continues to be a pervasive public health issue with approximately 11% of women experiencing rape while in college. As such, it is important to examine factors unique to college campuses that influence the occurrences of rape. Methods: Using data from 1,423 four-year universities (public and private with at…

  8. 161 ETHICAL PROBLEMS OF RAPE AS A SOCIAL CANKERWORM ...

    African Journals Online (AJOL)

    the child after collecting same willingly give in for it with the adult, it is said to be rape as well. ... in their teens, while others are matured adults (Obasi, 2007). ... Christian teen girls are no less likely to become victims of the rape". Rape cases are not limited to Nigeria alone. Other countries of the globe have their own side of ...

  9. Counting the countless – rape victimisation during the Rwandan Genocide

    NARCIS (Netherlands)

    Bijleveld, C.C.J.H.; Morssinkhof, A.; Smeulers, A.L.

    2009-01-01

    Rape is regularly committed during a period of collective violence such as war. The article discusses the Rwandan genocide during which rape was used with the deliberate intent to destroy in whole or in part the Tutsi community. Rape is not often studied in such particular contexts, so little is

  10. Race and Rape: The Black Woman as Legitimate Victim.

    Science.gov (United States)

    Williams, Linda Meyer

    Scientific investigations of the relationship between race and rape have been flawed by the acceptance of official statistics and have been influenced by prevailing myths about rape and race. This paper proposes a theoretical framework for understanding rape and race. The thesis is presented that only the black victim of sexual assault is viewed…

  11. Resisting rape: the effects of victim self-protection on rape completion and injury.

    Science.gov (United States)

    Tark, Jongyeon; Kleck, Gary

    2014-03-01

    The impact of victim resistance on rape completion and injury was examined utilizing a large probability sample of sexual assault incidents, derived from the National Crime Victimization Survey (1992-2002), and taking into account whether harm to the victim followed or preceded self-protection (SP) actions. Additional injuries besides rape, particularly serious injuries, following victim resistance are rare. Results indicate that most SP actions, both forceful and nonforceful, reduce the risk of rape completion, and do not significantly affect the risk of additional injury.

  12. The Psychology of the Politics of Rape: Political Ideology, Moral Foundations, and Attitudes Toward Rape.

    Science.gov (United States)

    Barnett, Michael D; Hilz, Emily N

    2017-05-01

    Previous research has found that conservatives and liberals emphasize different moral foundations. The purpose of these two studies was to investigate whether moral foundations mediate the relationship between political ideology and attitudes toward rape among U.S. college students. In Study 1, moral foundations fully mediated the relationship between political ideology and rape myth acceptance. Study 2 generally replicated the results of Study 1, with binding foundations demonstrating the most consistent mediating effects. These results suggest that individual differences in moral decision-making may explain the relationship between political ideology and attitudes toward rape.

  13. Rape trauma syndrome in the military courts.

    Science.gov (United States)

    Young, S A

    1995-01-01

    The military courts have developed a rich case law tradition in the area of rape trauma syndrome testimony. These cases are particularly important in the context of a military that is both increasingly female and increasingly sensitive to mixed gender relationships. This article reviews the military court's approach to rape trauma testimony over the past 15 years. The author notes that military courts have been accepting of this testimony within certain well defined limits. The author analyzes the approach to testimony at one military medical center and offers a testimony model for the forensic psychiatrist who testifies in a military setting.

  14. Perceptions of, and Assistance Provided to, a Hypothetical Rape Victim: Differences between Rape Disclosure Recipients and Nonrecipients

    Science.gov (United States)

    Paul, Lisa A.; Kehn, Andre; Gray, Matt J.; Salapska-Gelleri, Joanna

    2014-01-01

    Objective: Undergraduate rape disclosure recipients' and nonrecipients' sociodemographic and life experience variables, attitudes towards rape, and responses to a hypothetical rape disclosure were compared to determine differences between them. Participants: One hundred ninety-two undergraduates at 3 universities participated in this online survey…

  15. Examining the Relationship between Male Rape Myth Acceptance, Female Rape Myth Acceptance, Victim Blame, Homophobia, Gender Roles, and Ambivalent Sexism

    Science.gov (United States)

    Davies, Michelle; Gilston, Jennifer; Rogers, Paul

    2012-01-01

    The relationship between male rape myth acceptance, female rape myth acceptance, attitudes toward gay men, a series of gender role and sexism measures, victim blame and assault severity were investigated. It was predicted that men would display more negative, stereotypical attitudes than women and that male rape myth endorsement would be related…

  16. Genetic and Epigenetic Changes in Oilseed Rape (Brassica napus L.) Extracted from Intergeneric Allopolyploid and Additions with Orychophragmus

    OpenAIRE

    Gautam, Mayank; Dang, Yanwei; Ge, Xianhong; Shao, Yujiao; Li, Zaiyun

    2016-01-01

    ABSTRACT Allopolyploidization with the merger of the genomes from different species has been shown to be associated with genetic and epigenetic changes. But the maintenance of such alterations related to one parental species after the genome is extracted from the allopolyploid remains to be detected. In this study, the genome of Brassica napus L. (2n=38, genomes AACC) was extracted from its intergeneric allohexaploid (2n=62, genomes AACCOO) with another crucifer Orychophragmus violaceus (2n=2...

  17. Genetic and Epigenetic Changes in Oilseed Rape (Brassica napus L.) Extracted from Intergeneric Allopolyploid and Additions with Orychophragmus.

    Science.gov (United States)

    Gautam, Mayank; Dang, Yanwei; Ge, Xianhong; Shao, Yujiao; Li, Zaiyun

    2016-01-01

    Allopolyploidization with the merger of the genomes from different species has been shown to be associated with genetic and epigenetic changes. But the maintenance of such alterations related to one parental species after the genome is extracted from the allopolyploid remains to be detected. In this study, the genome of Brassica napus L. (2n = 38, genomes AACC) was extracted from its intergeneric allohexaploid (2n = 62, genomes AACCOO) with another crucifer Orychophragmus violaceus (2n = 24, genome OO), by backcrossing and development of alien addition lines. B. napus-type plants identified in the self-pollinated progenies of nine monosomic additions were analyzed by the methods of amplified fragment length polymorphism, sequence-specific amplified polymorphism, and methylation-sensitive amplified polymorphism. They showed modifications to certain extents in genomic components (loss and gain of DNA segments and transposons, introgression of alien DNA segments) and DNA methylation, compared with B. napus donor. The significant differences in the changes between the B. napus types extracted from these additions likely resulted from the different effects of individual alien chromosomes. Particularly, the additions which harbored the O. violaceus chromosome carrying dominant rRNA genes over those of B. napus tended to result in the development of plants which showed fewer changes, suggesting a role of the expression levels of alien rRNA genes in genomic stability. These results provided new cues for the genetic alterations in one parental genome that are maintained even after the genome becomes independent.

  18. Genetic and epigenetic changes in oilseed rape (Brassica napus L. extracted from intergeneric allopolyploid and additions with Orychophragmus

    Directory of Open Access Journals (Sweden)

    Mayank eGautam

    2016-04-01

    Full Text Available ABSTRACT Allopolyploidization with the merger of the genomes from different species has been shown to be associated with genetic and epigenetic changes. But the maintenance of such alterations related to one parental species after the genome is extracted from the allopolyploid remains to be detected. In this study, the genome of Brassica napus L. (2n=38, genomes AACC was extracted from its intergeneric allohexaploid (2n=62, genomes AACCOO with another crucifer Orychophragmus violaceus (2n=24, genome OO, by backcrossing and development of alien addition lines. B. napus-type plants identified in the self-pollinated progenies of nine monosomic additions were analyzed by the methods of amplified fragment length polymorphism (AFLP, sequence-specific amplified polymorphism (SSAP, and methylation-sensitive amplified polymorphism (MSAP. They showed modifications to certain extents in genomic components (loss and gain of DNA segments and transposons, introgression of alien DNA segments and DNA methylation, compared with B. napus donor. The significant differences in the changes between the B. napus types extracted from these additions likely resulted from the different effects of individual alien chromosomes. Particularly, the additions which harbored the O. violaceus chromosome carrying dominant rRNA genes over those of B. napus tended to result in the development of plants which showed fewer changes, suggesting a role of the expression levels of alien rRNA genes in genomic stability. These results provided new cues for the genetic alterations in one parental genome that are maintained even after the genome becomes independent.

  19. Removing the mustard oil bomb from seeds: transgenic ablation of myrosin cells in oilseed rape (Brassica napus) produces MINELESS seeds.

    Science.gov (United States)

    Borgen, Birgit Hafeld; Thangstad, Ole Petter; Ahuja, Ishita; Rossiter, John Trevor; Bones, Atle Magnar

    2010-06-01

    Many plant phytochemicals constitute binary enzyme-glucoside systems and function in plant defence. In brassicas, the enzyme myrosinase is confined to specific myrosin cells that separate the enzyme from its substrate; the glucosinolates. The myrosinase-catalysed release of toxic and bioactive compounds such as isothiocyanates, upon activation or tissue damage, has been termed 'the mustard oil bomb' and characterized as a 'toxic mine' in plant defence. The removal of myrosin cells and the enzyme that triggers the release of phytochemicals have been investigated by genetically modifying Brassica napus plants to remove myrosinase-storing idioblasts. A construct with the seed myrosin cell-specific Myr1.Bn1 promoter was used to express a ribonuclease, barnase. Transgenic plants ectopically expressing barnase were embryo lethal. Co-expressing barnase under the control of the Myr1.Bn1 promoter with the barnase inhibitor, barstar, under the control of the cauliflower mosaic virus 35S promoter enabled a selective and controlled death of myrosin cells without affecting plant viability. Ablation of myrosin cells was confirmed with light and electron microscopy, with immunohistological analysis and immunogold-electron microscopy analysis showing empty holes where myrosin cells normally are localized. Further evidence for a successful myrosin cell ablation comes from immunoblots showing absence of myrosinase and negligible myrosinase activity, and autolysis experiments showing negligible production of glucosinolate hydrolysis products. The plants where the myrosin defence cells have been ablated and named 'MINELESS plants'. The epithiospecifier protein profile and glucosinolate levels were changed in MINELESS plants, pointing to localization of myrosinases and a 35 kDa epithiospecifier protein in myrosin cells and a reduced turnover of glucosinolates in MINELESS plants.

  20. Assessing quantitative resistance against Leptosphaeria maculans (phoma stem canker in Brassica napus (oilseed rape in young plants.

    Directory of Open Access Journals (Sweden)

    Yong-Ju Huang

    Full Text Available Quantitative resistance against Leptosphaeria maculans in Brassica napus is difficult to assess in young plants due to the long period of symptomless growth of the pathogen from the appearance of leaf lesions to the appearance of canker symptoms on the stem. By using doubled haploid (DH lines A30 (susceptible and C119 (with quantitative resistance, quantitative resistance against L. maculans was assessed in young plants in controlled environments at two stages: stage 1, growth of the pathogen along leaf veins/petioles towards the stem by leaf lamina inoculation; stage 2, growth in stem tissues to produce stem canker symptoms by leaf petiole inoculation. Two types of inoculum (ascospores; conidia and three assessment methods (extent of visible necrosis; symptomless pathogen growth visualised using the GFP reporter gene; amount of pathogen DNA quantified by PCR were used. In stage 1 assessments, significant differences were observed between lines A30 and C119 in area of leaf lesions, distance grown along veins/petioles assessed by visible necrosis or by viewing GFP and amount of L. maculans DNA in leaf petioles. In stage 2 assessments, significant differences were observed between lines A30 and C119 in severity of stem canker and amount of L. maculans DNA in stem tissues. GFP-labelled L. maculans spread more quickly from the stem cortex to the stem pith in A30 than in C119. Stem canker symptoms were produced more rapidly by using ascospore inoculum than by using conidial inoculum. These results suggest that quantitative resistance against L. maculans in B. napus can be assessed in young plants in controlled conditions. Development of methods to phenotype quantitative resistance against plant pathogens in young plants in controlled environments will help identification of stable quantitative resistance for control of crop diseases.

  1. Assessing quantitative resistance against Leptosphaeria maculans (phoma stem canker) in Brassica napus (oilseed rape) in young plants.

    Science.gov (United States)

    Huang, Yong-Ju; Qi, Aiming; King, Graham J; Fitt, Bruce D L

    2014-01-01

    Quantitative resistance against Leptosphaeria maculans in Brassica napus is difficult to assess in young plants due to the long period of symptomless growth of the pathogen from the appearance of leaf lesions to the appearance of canker symptoms on the stem. By using doubled haploid (DH) lines A30 (susceptible) and C119 (with quantitative resistance), quantitative resistance against L. maculans was assessed in young plants in controlled environments at two stages: stage 1, growth of the pathogen along leaf veins/petioles towards the stem by leaf lamina inoculation; stage 2, growth in stem tissues to produce stem canker symptoms by leaf petiole inoculation. Two types of inoculum (ascospores; conidia) and three assessment methods (extent of visible necrosis; symptomless pathogen growth visualised using the GFP reporter gene; amount of pathogen DNA quantified by PCR) were used. In stage 1 assessments, significant differences were observed between lines A30 and C119 in area of leaf lesions, distance grown along veins/petioles assessed by visible necrosis or by viewing GFP and amount of L. maculans DNA in leaf petioles. In stage 2 assessments, significant differences were observed between lines A30 and C119 in severity of stem canker and amount of L. maculans DNA in stem tissues. GFP-labelled L. maculans spread more quickly from the stem cortex to the stem pith in A30 than in C119. Stem canker symptoms were produced more rapidly by using ascospore inoculum than by using conidial inoculum. These results suggest that quantitative resistance against L. maculans in B. napus can be assessed in young plants in controlled conditions. Development of methods to phenotype quantitative resistance against plant pathogens in young plants in controlled environments will help identification of stable quantitative resistance for control of crop diseases.

  2. Effects of Single and Multifactor Treatments with Elevated Temperature, CO2 and Ozone on Oilseed Rape and Barley

    DEFF Research Database (Denmark)

    Clausen, Sabine Karin; Frenck, Georg; van der Linden, Leon Gareth

    2011-01-01

    barley (Hordeum vulgare L.). Seven genotypes of each species were cultivated in six single- and multifactor treatments with ambient or elevated CO2 (385 ppm and 700 ppm), O3 (20 ppb and 60 ppb) and temperature (12/19 °C and 17/24 °C). Growth and production parameters were measured. Elevated CO2 increased...

  3. Progressive introgression between ¤Brassica napus¤ (oilseed rape) and ¤B-rapa¤

    DEFF Research Database (Denmark)

    Hansen, L.B.; Siegismund, H.R.; Bagger Jørgensen, Rikke

    2003-01-01

    that introgression can lead to both (1) exchange of chloroplast DNA between species producing B. rapa-like plants with B. napus chloroplasts and (2) incorporation of B. napus C-genome DNA into the B. rapa genome. Therefore, we question whether it can be regarded as containment to position transgenes...... population with the same AFLP-markers; AFLP data were supported by chromosome counting. We also analysed the offspring with a species-specific chloroplast marker and finally evaluated the reproductive system in selected maternal plants. Our results indicated a high outcrossing rate of the introgressed...

  4. Genetic load and transgenic mitigating genes in transgenic Brassica rapa (field mustard × Brassica napus (oilseed rape hybrid populations

    Directory of Open Access Journals (Sweden)

    Warwick Suzanne I

    2009-10-01

    Full Text Available Abstract Background One theoretical explanation for the relatively poor performance of Brassica rapa (weed × Brassica napus (crop transgenic hybrids suggests that hybridization imparts a negative genetic load. Consequently, in hybrids genetic load could overshadow any benefits of fitness enhancing transgenes and become the limiting factor in transgenic hybrid persistence. Two types of genetic load were analyzed in this study: random/linkage-derived genetic load, and directly incorporated genetic load using a transgenic mitigation (TM strategy. In order to measure the effects of random genetic load, hybrid productivity (seed yield and biomass was correlated with crop- and weed-specific AFLP genomic markers. This portion of the study was designed to answer whether or not weed × transgenic crop hybrids possessing more crop genes were less competitive than hybrids containing fewer crop genes. The effects of directly incorporated genetic load (TM were analyzed through transgene persistence data. TM strategies are proposed to decrease transgene persistence if gene flow and subsequent transgene introgression to a wild host were to occur. Results In the absence of interspecific competition, transgenic weed × crop hybrids benefited from having more crop-specific alleles. There was a positive correlation between performance and number of B. napus crop-specific AFLP markers [seed yield vs. marker number (r = 0.54, P = 0.0003 and vegetative dry biomass vs. marker number (r = 0.44, P = 0.005]. However under interspecific competition with wheat or more weed-like conditions (i.e. representing a situation where hybrid plants emerge as volunteer weeds in subsequent cropping systems, there was a positive correlation between the number of B. rapa weed-specific AFLP markers and seed yield (r = 0.70, P = 0.0001, although no such correlation was detected for vegetative biomass. When genetic load was directly incorporated into the hybrid genome, by inserting a fitness-mitigating dwarfing gene that that is beneficial for crops but deleterious for weeds (a transgene mitigation measure, there was a dramatic decrease in the number of transgenic hybrid progeny persisting in the population. Conclusion The effects of genetic load of crop and in some situations, weed alleles might be beneficial under certain environmental conditions. However, when genetic load was directly incorporated into transgenic events, e.g., using a TM construct, the number of transgenic hybrids and persistence in weedy genomic backgrounds was significantly decreased.

  5. Transcriptomic analysis of nitrogen starvation- and cultivar-specific leaf senescence in winter oilseed rape (Brassica napus L.).

    Science.gov (United States)

    Koeslin-Findeklee, Fabian; Rizi, Vajiheh Safavi; Becker, Martin A; Parra-Londono, Sebastian; Arif, Muhammad; Balazadeh, Salma; Mueller-Roeber, Bernd; Kunze, Reinhard; Horst, Walter J

    2015-04-01

    High nitrogen (N) efficiency, characterized by high grain yield under N limitation, is an important agricultural trait in Brassica napus L. cultivars related to delayed senescence of older leaves during reproductive growth (a syndrome called stay-green). The aim of this study was thus to identify genes whose expression is specifically altered during N starvation-induced leaf senescence and that can be used as markers to distinguish cultivars at early stages of senescence prior to chlorophyll loss. To this end, the transcriptomes of leaves of two B. napus cultivars differing in stay-green characteristics and N efficiency were analyzed 4 days after the induction of senescence by either N starvation, leaf shading or detaching. In addition to N metabolism genes, N starvation mostly (and specifically) repressed genes related to photosynthesis, photorespiration and cell-wall structure, while genes related to mitochondrial electron transport and flavonoid biosynthesis were predominately up-regulated. A kinetic study over a period of 12 days with four B. napus cultivars differing in their stay-green characteristics confirmed the cultivar-specific regulation of six genes in agreement with their senescence behavior: the senescence regulator ANAC029, the anthocyanin synthesis-related genes ANS and DFR-like1, the ammonium transporter AMT1;4, the ureide transporter UPS5, and SPS1 involved in sucrose biosynthesis. The identified genes represent markers for the detection of cultivar-specific differences in N starvation-induced leaf senescence and can thus be employed as valuable tools in B. napus breeding. Copyright © 2015 Elsevier Ireland Ltd. All rights reserved.

  6. To be or not to be biosafe : an evaluation of transgenic phosphinothricin-tolerant oilseed rape (Brassica napus L.)

    NARCIS (Netherlands)

    Metz, P.L.J.

    1997-01-01

    Genetic modification is an additional tool for conventional plant breeding to improve the application and quality of crop plants. No longer hampered by natural crossing barriers, application of genetic modification results in a nearly infinite pool from which genes, after isolation, can be

  7. Leaf Senescence, Root Morphology, and Seed Yield of Winter Oilseed Rape (Brassica napus L. at Varying Plant Densities

    Directory of Open Access Journals (Sweden)

    Ming Li

    2017-01-01

    Full Text Available In this study, the yield and yield components were studied using a conventional variety Zhongshuang 11 (ZS 11 and a hybrid variety Zhongyouza 12 (ZYZ 12 at varying plant densities. The increase in plant density led to an initial increase in seed yield and pod numbers per unit area, followed by a decrease. The optimal plant density was 58.5 × 104 plants ha−1 in both ZS 11 and ZYZ 12. The further researches on physiological traits showed a rapid decrease in the green leaf area index (GLAI and chlorophyll content and a remarkable increase in malondialdehyde content in high plant density (HPD population than did the low plant density (LPD population, which indicated the rapid leaf senescence. However, HPD had higher values in terms of pod area index (PAI, pod photosynthesis, and radiation use efficiency (RUE after peak anthesis. A significantly higher level of dry matter accumulation and nitrogen utilization efficiency were observed, which resulted in higher yield. HPD resulted in a rapid decrease in root morphological parameters (root length, root tips, root surface area, and root volume. These results suggested that increasing the plant density within a certain range was a promising option for high seed yield in winter rapeseed in China.

  8. Development of a biologically based fertilizer, incorporating Bacillus megaterium A6, for improved phosphorus nutrition of oilseed rape

    National Research Council Canada - National Science Library

    Hu, Xiaojia; Roberts, Daniel P; Xie, Lihua; Maul, Jude E; Yu, Changbing; Li, Yinshui; Zhang, Shujie; Liao, Xing

    2013-01-01

    ... strain A6 in 1 of 2 experiments, suggesting a role for strain A6 in improving yield. Strain A6 was capable of solubilizing phosphorus from rock phosphate in liquid culture and produced enzymes capable of mineralizing organic phosphorus...

  9. Incapacitated rape and alcohol use: a prospective analysis.

    Science.gov (United States)

    Kaysen, Debra; Neighbors, Clayton; Martell, Joel; Fossos, Nicole; Larimer, Mary E

    2006-10-01

    This study examined timing of alcohol-related sexual assaults (incapacitated rape) in relation to both alcohol consumption and alcohol-related negative consequences. The sample was drawn from a randomly selected pool of college students across three campuses (n=1238) followed over a three year time period. 91% of students never experienced an incapacitated rape, 2% reported an incapacitated rape prior to the first assessment point (n=30), and 6% reported one over the course of the study (n=76). Results indicated that incapacitated rape was associated with higher alcohol use and more negative consequences in the years prior to the assault. Incapacitated rape was also associated with higher alcohol use and more negative consequences during the year in which the rape took place and subsequent years, with highest rates measured for the year of the rape. These results suggest alcohol use can function as both risk factor and consequence of sexual victimization.

  10. Reader responses to literary depictions of rape

    NARCIS (Netherlands)

    E.M. Koopman (Emy); M. Hilscher (Michelle); G.C. Cupchik (Gerald)

    2012-01-01

    textabstractThis study explored reader responses to different literary depictions of rape. Four literary excerpts were used and divided as aesthetic versus nonaesthetic (style) and allusive versus explicit (detail). The general question was how readers would react to literary fragments depicting

  11. Ethical challenges when reading aesthetic rape scenes

    NARCIS (Netherlands)

    E.M. Koopman (Emy)

    2011-01-01

    textabstractBoth the issue of the ethics of representation and the issue of the ethics of reading are particularly important when it comes to representations of suffering and violence. This chapter addresses the ethics of representing and the ethics of reading rape, with a focus on the latter.

  12. Alcohol Consumption and Responsibility Judgment for Rape.

    Science.gov (United States)

    Kreutzer, Jeffrey S.; And Others

    One explanation for the positive relationship between alcohol consumption and violent crime considers alcohol consumption to be a socially accepted excuse for otherwise inappropriate behaviors. A policy capturing methodology was used to examine judgments of responsibility for rape. Male (N=30) and female (N=66) undergraduates received 40 profiles…

  13. Taking a chance: Sex, alcohol & acquaintance rape

    DEFF Research Database (Denmark)

    Demant, Jakob; Heinskou, Marie Bruvik

    2011-01-01

    to grasp more of the complexities of the situation. By analyzing a single case of acquaintance rape and then reflect this case in the light of interviews on young people's drinking habits it is argued that the complexities within the analytical frame of chance is crucial in understanding reported...

  14. Rape Myths and the Cross-Cultural Adaptation of the Illinois Rape Myth Acceptance Scale in China.

    Science.gov (United States)

    Xue, Jia; Fang, Gang; Huang, Hui; Cui, Naixue; Rhodes, Karin V; Gelles, Richard

    2016-06-05

    The study examines the similarities and differences between China and the United States with regard to rape myths. We assessed the individual level of rape myth acceptance among Chinese university students by adapting and translating a widely used measure of rape myth endorsement in the United States, the Illinois Rape Myth Acceptance (IRMA) scale. We assessed whether the IRMA scale would be an appropriate assessment of attitudes toward rape among young adults in China. The sample consisted of 975 Chinese university students enrolled in seven Chinese universities. We used explorative factor analysis to examine the factor structure of the Chinese translation of the IRMA scale. Results suggest that the IRMA scale requires some modification to be employed with young adults in China. Our analyses indicate that 20 items should be deleted, and a five-factor model is generated. We discuss relevant similarities and differences in the factor structure and item loadings between the Chinese Rape Myth Acceptance (CRMA) and the IRMA scales. A revised version of the IRMA, the CRMA, can be used as a resource in rape prevention services and rape victim support services. Future research in China that employs CRMA will allow researchers to examine whether individual's response to rape myth acceptance can predict rape potential and judgments of victim blaming and community members' acceptance of marital rape. © The Author(s) 2016.

  15. Contact with Counter-Stereotypical Women Predicts Less Sexism, Less Rape Myth Acceptance, Less Intention to Rape (in Men) and Less Projected Enjoyment of Rape (in Women).

    Science.gov (United States)

    Taschler, Miriam; West, Keon

    2017-01-01

    Intergroup contact-(positive) interactions with people from different social groups-is a widely researched and strongly supported prejudice-reducing mechanism shown to reduce prejudice against a wide variety of outgroups. However, no known previous research has investigated whether intergroup contact can also reduce sexism against women. Sexism has an array of negative outcomes. One of the most detrimental and violent ones is rape, which is both justified and downplayed by rape myth acceptance. We hypothesised that more frequent, higher quality contact with counter-stereotypical women would predict lower levels of sexism and thus less rape myth acceptance (in men) and less sexualised projected responses to rape (in women). Two studies using online surveys with community samples supported these hypotheses. In Study 1, 170 male participants who experienced more positive contact with counter-stereotypical women reported less intention to rape. Similarly, in Study 2, 280 female participants who experienced more positive contact with counter-stereotypical women reported less projected sexual arousal at the thought of being raped. Thus, the present research is the first known to show that contact could be a potential tool to combat sexism, rape myth acceptance, intentions to rape in men, and sexualisation of rape by women.

  16. In vitro Fermentation, Digestion Kinetics and Methane Production of Oilseed Press Cakes from Biodiesel Production.

    Science.gov (United States)

    Olivares-Palma, S M; Meale, S J; Pereira, L G R; Machado, F S; Carneiro, H; Lopes, F C F; Maurício, R M; Chaves, A V

    2013-08-01

    Following the extraction of oil for biodiesel production, oilseed press cakes are high in fat. As the dietary supplementation of fat is currently considered the most promising strategy of consistently depressing methanogenesis, it follows that oilseed press cakes may have a similar potential for CH4 abatement. As such, this study aimed to characterise the nutritive value of several oilseed press cakes, glycerine and soybean meal (SBM) and to examine their effects on in vitro ruminal fermentation, digestion kinetics and CH4 production. Moringa press oil seeds exhibited the greatest in sacco effective degradability (ED) of DM and CP (pMoringa oilseed press cakes produced the lowest CH4 (mg/g digested DM) at 6 and 12 h of incubation (pmoringa oilseed press cake at 400 g/kg DM has the greatest potential of the oilseed press cakes examined in this study, to reduce CH4 production, without adversely affecting nutrient degradability.

  17. Fermentation, Digestion Kinetics and Methane Production of Oilseed Press Cakes from Biodiesel Production

    OpenAIRE

    S. M. Olivares-Palma; S. J. Meale; PEREIRA, L. G. R.; MACHADO, F. S.; Carneiro, H.; F.C.F. Lopes; MAURÍCIO, R. M.; Chaves, A. V.

    2013-01-01

    Following the extraction of oil for biodiesel production, oilseed press cakes are high in fat. As the dietary supplementation of fat is currently considered the most promising strategy of consistently depressing methanogenesis, it follows that oilseed press cakes may have a similar potential for CH4 abatement. As such, this study aimed to characterise the nutritive value of several oilseed press cakes, glycerine and soybean meal (SBM) and to examine their effects on in vitro ruminal fermentat...

  18. The Effect of Biodiesel Policies on World Biodiesel and Oilseed Prices

    OpenAIRE

    de Gorter, Harry; Drabik, Dusan; Timilsina, Govinda R.

    2014-01-01

    A theoretical and empirical model is developed to analyze the effect of a biodiesel mandate, a tax exemption (tax credit) and an exogenous diesel price shock on world soybean and canola markets. The jointness in crushing oil and meal from the oilseed reduces the size of the link between biodiesel and oilseed prices. A diesel price shock with a mandate results in a smaller change in oilseed prices compared with a tax exemption. Higher diesel prices increase biodiesel prices under a tax exempti...

  19. Applications of Enzymes in Oil and Oilseed Processing

    DEFF Research Database (Denmark)

    Xu, Xuebing

    conventionally high temperature conditioning or cooking is necessary. The good story in industry is the fish oil and olive oil processing. Good quality and higher oil yield have been achieved through the use of enzymes in the processing stages. For the refining stage, the use of enzymes for degumming has......Enzymes, through the last 20-30 years research and development, have been widely explored for the uses in oil and oilseed processing. Following the conventional processing technology from oilseeds, the oil can be produced through pressing or solvent extraction. The crude oil is then refined to meet...... edible requirements. The oil can be also modified to meet functional or even nutritional needs. In each of those steps, enzymes have been used in industry successfully. For the oil processing stage, enzymes have been used to destroy the cell structure so that makes the oil release easier, where...

  20. Study of Pea Accessions for Development of an Oilseed Pea

    OpenAIRE

    Ehsan Khodapanahi; Mark Lefsrud; Valerie Orsat; Jaswinder Singh; Tom D. Warkentin

    2012-01-01

    Global interest in stable energy resources coupled with growing demand for bio-oils in various conventional and arising industries has renewed the importance of vegetable oil production. To address this global interest, oilseed production has been increased in recent decades by different approaches, such as extending the cultivation area of oil crops, or breeding and growing genetically modified plants. In this study, pea ( Pisum sativum L.) accessions were screened for lipid content using a ...

  1. Occurrence of aflatoxins in oilseeds providing cocoa-butter substitutes.

    Science.gov (United States)

    Kershaw, S J

    1982-01-01

    Four oilseeds providing cocoa-butter substitutes--shea, pentadecima, illipe, and salseed--when tested as substrates for aflatoxin production by two strains of Aspergillus parasiticus, gave varying levels of aflatoxin. Aflatoxins were found at low levels occurring naturally in moldy shea-nuts, but none of 21 commercial shea-nut samples contained greater than 20 micrograms of aflatoxin B1 per kg. PMID:6808919

  2. Insecticide residues cross-contamination of oilseeds during storage

    Directory of Open Access Journals (Sweden)

    Dauguet Sylvie

    2007-11-01

    Full Text Available Pesticide residues are found in oilseeds and crude oils: they are mainly organophosphate insecticides (pirimiphos-methyl, dichlorvos, malathion used in empty storage facilities and for application to stored cereal grains. Even if pests are found in stored oilseeds, French regulation does not permit use of these insecticides on stored oilseeds, as they have affinity for these lipophilic subtances. These residues arise from cross-contamination during mechanical contact with storage bins and grain handling equipment, and not from illegal use. This uptake of insecticide residues from their storage environment by oilseeds can lead to levels that exceed regulatory limits. An investigation of 11 grain storage companies allowed us to follow the course of 27 sunflower seeds batches, from reception at the storage facilities to outloading. Samples from each of these batches, made at outloading, were analysed content for insecticide residues. Traceability of sunflower seeds established by storers allowed us to identify the origine of observed cross-contamination cases. Substances discovered were dichlorvos, pirimiphos-methyl and malathion (and chlorpyriphos-methyl in a single case. Pirimiphos-methyl was most commonly detected, but most cases of non-accordance with regulatory levels were observed with dichlorvos and malathion. Main cross-contamination hazard resulted from treatment of cereals at outloading, just before sunflower seeds were outloaded, especially when these cereals treatments were frequent on that elevator. Other situations led to cross-contaminations, but generally of lower levels: outloading of sunflower seeds after outloading of cereal that was treated at the reception, several weeks or months before; sunflower seeds stored in bin that contained previously treated cereal; empty bins and handling equipment treated before receipt of sunflower seeds.

  3. Criminalizing rape within marriage: perspectives of Ghanaian university students.

    Science.gov (United States)

    Adinkrah, Mensah

    2011-09-01

    Forcing sexual intercourse on an unwilling marital partner, or marital rape, is not a crime in many societies around the world, because of a marital exemption rule that prohibits the prosecution of husbands who rape their wives. Concurrently, marital rape is one of the least studied phenomena in sexual violence research. This is particularly true for societies in the non-Western world. The current study examined the general attitudes of a sample of university students in Ghana, a West African country, toward marital rape. Respondents were also asked whether an ongoing legislative effort to criminalize marital rape in the country was warranted. The results indicated strong opposition toward criminalization. The results also indicated no marked differences between male and female respondents in attitudes toward marital rape and the need for a legislative response to the phenomenon. Patriarchal ideologies such as wifely submission to the husband and an implicit duty to provide sex in marriage provided some of the justifications furnished for why marital rape should remain noncriminalized. Advocates of criminalization mentioned the social, physical, and psychological effects of rape and how the enactment of marital rape legislation and the imposition of severe criminal sanctions would help prevent the incidence of marital rape and other forms of violence against women in the society.

  4. Study of Pea Accessions for Development of an Oilseed Pea

    Directory of Open Access Journals (Sweden)

    Ehsan Khodapanahi

    2012-09-01

    Full Text Available Global interest in stable energy resources coupled with growing demand for bio-oils in various conventional and arising industries has renewed the importance of vegetable oil production. To address this global interest, oilseed production has been increased in recent decades by different approaches, such as extending the cultivation area of oil crops, or breeding and growing genetically modified plants. In this study, pea (Pisum sativum L. accessions were screened for lipid content using a rapid extraction method. This method quantifies lipid concentration in pea seeds and was developed by assessing and comparing the results of existing extraction methods used for canola and soybean, the top two Canadian oilseeds. Seeds of 151 field pea accessions were grown to maturity in 2009 and 2010 at McGill University (Quebec, Canada. Overall, lipid concentration in pea seeds ranged from 0.9 to 5.0%. Among several seed characteristics, only seed shape (wrinkled verses round had a significant effect on the total lipid production in the seeds. Peas are a valuable source of protein and starch, but the lipid concentration in their seeds has been undervalued. This research supports the idea of developing a novel dual-purpose oilseed pea that emulates the protein and oil production in soybean seeds while being conveniently adapted to a colder climate.

  5. Exploring different explanatory psychological models of perpetrators of rape

    OpenAIRE

    2008-01-01

    M.A. Without prejudice to race or culture, rape is the hurt of both males and females of all ages. Rape can be one of the most devastating emotional events in a person’s life, the effects of which are entrenched for life (Rozee & Koss, 2001). Motives underscoring the need to commit rape have received much attention in the literature. Evolutionary theorists such as Thornhill and Palmer (2000) report that sexual needs and reproduction are the primary motivational factors. Feminist theorists ...

  6. Communicating/Muting Date Rape: A Co-Cultural Theoretical Analysis of Communication Factors Related to Rape Culture on a College Campus

    Science.gov (United States)

    Burnett, Ann; Mattern, Jody L.; Herakova, Liliana L.; Kahl, David H., Jr.; Tobola, Cloy; Bornsen, Susan E.

    2009-01-01

    Previous studies suggest that college campuses foster a rape culture in which date rape (most commonly, rape of women) is an accepted part of campus activity (Buchwald, Fletcher, & Roth, 1993; Sanday, 2007). In focus groups at a Midwestern university, researchers asked students about rape as they experienced it or knew about it on campus. The…

  7. Rape: Past and present legal regulation

    Directory of Open Access Journals (Sweden)

    Đapović Lasta S.

    2002-01-01

    Full Text Available Rape was identified as a criminal act in the earliest known legal writings, Hamurabi's legal code (cir. 2000 BC. In the Balkans, in the Middle Ages, rape was always punished in one way or another, in the statutes of the Adriatic maritime towns, which were under the rule of the Nemanjić state or the West. All categories of women, even prostitutes and slaves, were protected by law from sexual violence in many statutes. Also in Dušan's legal code the regulation is found, severely punishing rapists. Laws following the Second Serbian Uprising, also provide severe punishment for sexual violence, especially towards women but also male minors. Legal and court practice, following the Second World War, indicate that the protection of women from sexual violence is completely inadequate and this at a time when women had achieved equal rights with men in many spheres of life. World events at the end of the 20th century: collapse of the Soviet Union: as well as the happenings in this region,: war, ethnic and religious conflicts resulted in massive rape of women, opening of brothels, trading women and family violence. Non state organizations, mainly women in expert associations, invest great effort to rectify this very unsatisfactory situation. They exert pressure for change in the law and in court practice, by which women would have more effective defense against sexual violence, as well as organized assistance for women victims of traffic, and warning of the possible danger.

  8. Ending Rape in War: How Far Have We Come?

    Directory of Open Access Journals (Sweden)

    Lucy Fiske

    2015-02-01

    Full Text Available The rape of women has for centuries been an endemic feature of war, yet perpetrators largely go unpunished. Women were sanctioned as the spoils of war in biblical times and more recently it has been claimed that it is more dangerous to be a woman than a soldier in modern conflict. Nevertheless, until the establishment of the ad hoc International Criminal Tribunals for Rwanda and the former Yugoslavia – there was very little concern regarding the need to address the rape of women in conflict. This paper briefly maps historical attitudes towards rape in war, outlines some analyses and explanations of why rape in war occurs and finally turns more substantively to recent efforts by the international community to prosecute rape as a war crime and a crime against humanity. We argue, that while commendable in some ways, contemporary approaches to rape in war risk reinforcing aspects of women’s status which contribute to the targeting of women for rape and continue to displace women from the centre to the margins in debates and practices surrounding rape in both war and peace time.  We conclude by arguing that criminal prosecutions alone are insufficient and that, if we are to end the rape of women and girls in war (and peace we need a radical restructuring of gender relations across every sphere of social and political life.

  9. Reactions to rape: a military forensic psychiatrist's perspective.

    Science.gov (United States)

    Ritchie, E C

    1998-08-01

    Accusations of rape or sexual harassment are currently very high-profile in the military. This article discusses rape allegations in the military legal system from a psychiatric perspective. The original definition of "rape trauma syndrome" and subsequent psychiatric thinking about the diagnosis are briefly outlined. Common reactions seen in military victims in this era are described. A prototypical military case is presented. An adequate evaluation of an alleged victim is outlined. Credentials and preparation of an expert witness are also briefly discussed, with cautions about the use of expert testimony in cases of alleged sexual assault and rape trauma syndrome.

  10. 7 CFR 1412.35 - Incorrect or false production evidence of oilseeds and pulse crops.

    Science.gov (United States)

    2010-01-01

    ... false production evidence of oilseeds and pulse crops. (a) If production evidence submitted in... 7 Agriculture 10 2010-01-01 2010-01-01 false Incorrect or false production evidence of oilseeds and pulse crops. 1412.35 Section 1412.35 Agriculture Regulations of the Department of Agriculture...

  11. In situ ruminal crude protein degradability of by-products from cereals, oilseeds and animal origin

    NARCIS (Netherlands)

    Habib, G.; Khan, N.A.; Ali, M.; Bezabih, M.

    2013-01-01

    The aim of this study was to establish a database on in situ ruminal crude protein (CP) degradability characteristics of by-products from cereal grains, oilseeds and animal origin commonly fed to ruminants in Pakistan and South Asian Countries. The oilseed by-products were soybean meal, sunflower

  12. Perceptions of stranger and acquaintance rape: the role of benevolent and hostile sexism in victim blame and rape proclivity.

    Science.gov (United States)

    Abrams, Dominic; Viki, G Tendayi; Masser, Barbara; Bohner, Gerd

    2003-01-01

    In Studies 1 and 2, after reading an acquaintance-rape but not a stranger-rape scenario, higher benevolent sexist but not hostile sexist participants blamed the victim significantly more. In Study 2, higher hostile sexist but not benevolent sexist male participants showed significantly greater proclivity to commit acquaintance (but not stranger) rape. Studies 3 and 4 supported the hypothesis that the effects of benevolent sexism and hostile sexism are mediated by different perceptions of the victim, as behaving inappropriately and as really wanting sex with the rapist. These findings show that benevolent sexism and hostile sexism underpin different assumptions about women that generate sexist reactions toward rape victims.

  13. Reduction of greenhouse gas emissions in the rape cultivation with special consideration of nitrogen fertilization; Minderung von Treibhausgasemissionen im Rapsanbau unter besonderer Beruecksichtigung der Stickstoffduengung

    Energy Technology Data Exchange (ETDEWEB)

    Heilmann, Hubert [Landesforschungsanstalt fuer Landwirtschaft und Fischerei Mecklenburg-Vorpommern, Guelzow-Pruezen (Germany). Inst. fuer Pflanzenproduktion und Betriebswirtschaft; Riemer, Doerte

    2017-08-01

    Involved into the research project ''Mitigation of greenhouse gas emissions in oilseed rape cropping with special consideration of nitrogen fertilizing'' regional specific GHG cropping emissions according to benchmark and regional experts are calculated by using a calculation method developed in cooperation with IFEU and according to IPCC (2006). The following results are achieved for 35 German NUTS2-regions: - nitrogen fertilization is the main influence for GHG emission reduction; - the use of low-emission nitrogen fertilizers is worth for GHG emission reduction; - without increasing the nutrient efficiency of organic fertilizers, GHG emission reductions are difficult to achieve in many regions; - GHG emission reduction/climate protection and realization of the WRRL or N-Saldo reduction come up to the same aim; - economic consequences of restrictive carbon mitigation can be compensated by slight price surcharges for certified raw material.

  14. OILSEEDS AS A PERSPECTIVE IN THE NUTRITION ON DAIRY FARM

    Directory of Open Access Journals (Sweden)

    Ágnes Süli

    2017-11-01

    Full Text Available In the last decades many researches were made to change the milk product food’s composition. The production of better fat-compound milk and dairy products became a goal in the name of health conscious nutrition. Our researches were motivated by non-adequate milk fat’s compound. With the optimal supplement of the feed can be increased the proportion of the polyunsaturated fatty acids and can decreased the unsaturated fatty acids. The object of our experiment to study the effect of natural-based feed additives, such as oilseeds (whole linseed, extruded linseed, whole rapeseed on the fatty acid composition of milk fat. Further information was gained about of oilseeds in specific amounts to be fitted into technology of an intensive dairy farm in practice. The feed supplements were whole, untreated rapeseed and linseed and cold extruded linseed as a part of total mixed ration. The level of the processing of feed supplements affected the fatty acid composition of milk fat. The effect of full fat linseed feed supplement was more significant than that of the cold extruded linseed, in the increase of the polyunsaturated fatty acids, with special regard to the increase of the α-linolenic acid proportion. Out of the full fat oilseeds the feeding of full fat rapeseed did not result in a considerable change in the fatty acid composition of milk fat. After applying full fat linseed as feed supplement, the proportion of α-linolenic acid, a conjugated linoleic acid, an eicosadienoic acid and a docosapentaenoic acid increased in the fatty acid composition of milk fat, while the concentration of many saturated fatty acids reduced

  15. Antioxidant activity of extracts obtained from residues of different oilseeds.

    Science.gov (United States)

    Matthäus, B

    2002-06-05

    Residues of the oil-extracting process of oilseeds contain bioactive substances such as phenolic compounds, which could be used as natural antioxidants for the protection of fats and oils against oxidative deterioration. Thus, the extraction of such constituents from residual material can be considered to contribute to the added value of these residues, which could justify their isolation. In the present work the fat-free residues of eight different oilseeds whose oils are usable for nutritional applications, and also as renewable resources, were extracted with 70% methanol, 70% acetone, water, and ethyl acetate/water, respectively. The resulting extracts were investigated regarding their content of total phenolic compounds by the Folin-Ciocalteau assay, sinapine, flavanoids, and the UV-absorption spectra. Further, the antioxidant activity of the extracts was characterized by the DPPH method, the beta-carotene-linoleic acid assay, and ESR investigations. The fat-free residues of the different oilseeds contained considerable amounts of extractable substances. The yields decreased with decreasing polarity of the solvent in the order water, 70% methanol, 70% acetone, and ethyl acetate. The ratio of total phenolic compounds to the extractable compounds ranged from 3 to 19%. There was no significant correlation between the amount of total extractable compounds and the total phenolic compounds (p < 0.001). All extracts showed remarkable antioxidant activities determined with the different methods. The effects depended strongly on the solvent used for the extraction as well as on the extracted residue. A correlation between the methods used for the characterization of the antioxidant activity and the composition of the residues could not be shown.

  16. Effects of oilseed supplements on milk production and quality in dairy cows fed a hay-based diet

    Directory of Open Access Journals (Sweden)

    Andreas Kasal

    2010-01-01

    Full Text Available The influence of rapeseed (Brassica napus L. and linseed (Linum usitatissimum L. on feed consumption, milk yield, composition and fatty acid (FA profile was investigated in lactating dairy cows. According to a Latin square design, twelve cows were assigned to three experimental settings which received a haybased diet supplemented with one of the following mixtures: cracked barley (1.0 kg and cracked maize (0.5 kg as Control diet (C-diet; full fat ground rapeseed (1.0 kg, cracked barley (1.0 kg and cracked maize (0.5 kg as Rapeseed diet (R-diet and extruded linseed (1.2 kg, cracked barley (1.0 kg and cracked maize (0.5 kg as Linseed diet (L-diet. Diet supplemented with rape- and linseed did not affect dry matter intake. As result of the high amount of dietary ether extract R- and L-diet showed higher crude fat intake as compared to the control. Despite the higher intake of crude fat and, in particular, of the polyunsaturated fraction, milk yield and composition resulted similar among treatments. Compared to C-diet, R-diet resulted in milk containing significantly lower amounts of saturated fatty acids (SFA (60.9 vs 66.9% of total detected SFA as well as higher amounts of monounsaturated fatty acids (MUFA (30.6 vs 24.2%. However, R-diet had no effect on polyunsaturated fatty acid content (PUFA. With respect to C-diet, L-diet also significantly reduced the content of SFA (59.8 vs 66.9% and increased both MUFA (29.1 vs 24.2% and PUFA (4.7 vs 4.1% contents. Feeding about 1 kg of whole oilseeds per head per day had no clear effects on milk vaccenic acid and conjugated linoleic acid contents. The inclusion of rapeseed in the diet significantly reduced the content of n-6 FA in the milk, whereas the linseed-supplemented diet significantly increased the content of n-3 FA. In comparison to C-diet, n-6/n-3 ratio was lower in L-diet milk and intermediate in R-diet milk. Feeding lactating dairy cows with oilseeds rich in unsaturated fatty acids (UFA did not

  17. Female University Student and Staff Perceptions of Rape

    Science.gov (United States)

    Herman, Michele H.; Sedlacek, William E.

    1974-01-01

    This study explores the fears of potential rape victims and the amount of information about rape and self-defense that such women possess. Many dimensions were found to explain the variability of responses among female members of the campus community. Suggestions for programs were offered. (Author/PC)

  18. "Corrective rape" of lesbians in the era of transformative ...

    African Journals Online (AJOL)

    There have been numerous incidents of “corrective” rape of lesbians in recent years. This article examines the adequacy of the existing South African legal framework to deal with incidents of “corrective” rape against the background of transformative constitutionalism. The various definitions of transformative ...

  19. Date rape among undergraduates in southwestern Nigeria federal ...

    African Journals Online (AJOL)

    Correlational survey design was adopted to examine the extent to which attitude towards date rape is associated with the respondents' emotional empathy, beliefs in rape myths and gender. Simple random sampling technique was adopted at three stages to draw one thousand two hundred and sixteen undergraduates ...

  20. Knowledge and Perceptions of Date Rape among Female ...

    African Journals Online (AJOL)

    AJRH Managing Editor

    (Afr J Reprod Health 2013; 17[3]: 137-148). Keywords: Female undergraduate students, Date rape perception, Date rape-related knowledge. Introduction. Violence against women is a pervasive human rights abuse worldwide1,2. It is any act that results in, or may result in, physical, sexual or psychological harm or suffering ...

  1. Rape Myth Beliefs and Bystander Attitudes among Incoming College Students

    Science.gov (United States)

    McMahon, Sarah

    2010-01-01

    Objective: The bystander approach to rape prevention is gaining popularity on college campuses, although research is limited. This study explored bystander attitudes and their relationship with rape myths in a sample of college students. Participants: Surveys from 2,338 incoming undergraduate students at a large, northeastern university were…

  2. The Experience of Male Rape in Non-Institutionalised Settings

    African Journals Online (AJOL)

    denise

    medical and psychological requirements of rape and sexual assault victims .... and can entail a higher degree of psychological manipulation rather ..... and happiness. Conclusion and Future Research. Among the primary observations evident in the descriptions obtained in this study is that the rape has tremendous power in ...

  3. A review of literature: rape and communication media strategies in ...

    African Journals Online (AJOL)

    Using historical-analytic methodology, this article reviews literature on rape and the communication strategies that can be used to address rape issues in Nigeria. In doing this, it critically engages communication theories as they apply to some communication media strategies that have been/can be deployed to redress the ...

  4. Rape Prevention with College Men: Evaluating Risk Status

    Science.gov (United States)

    Stephens, Kari A.; George, William H.

    2009-01-01

    This study evaluates the effectiveness of a theoretically based rape prevention intervention with college men who were at high or low risk to perpetrate sexually coercive behavior. Participants (N = 146) are randomly assigned to the intervention or control group. Outcomes include rape myth acceptance, victim empathy, attraction to sexual…

  5. Understanding the innermost nature of genocidal rape: A community ...

    African Journals Online (AJOL)

    This article shows that existing explanations of wartime rapes that are the biosocial, cultural and war strategy approaches do not capture sufficiently the innermost nature of genocidal rape. It proposes a new theoretical framework, the community-based approach, and confronts its premises with the real-life experiences of ...

  6. Genotypic variation of rape in phosphorus uptake from sparingly ...

    African Journals Online (AJOL)

    user

    2012-02-14

    Feb 14, 2012 ... were investigated to elucidate the contributions of root morphology and organic acids exudation to P uptake by rape from ... experiments, the activation capacity of insoluble Fe-P and Al-P of organic acids secreted by different rape genotypes roots ..... root morphology, root exudates, root architecture and.

  7. Page | 131 RISING CASES OF RAPE OFFENCES IN NIGERIA: NEW ...

    African Journals Online (AJOL)

    Fr. Ikenga

    connection is one which takes place otherwise than between husband and wife. A lot of issues arise ... It can therefore be said that rape is any sexual contact that is accompanied by penetration of whatever orifice of .... through its lens to look also at the Nigerian society which encourages offences of rape. It is a statement of ...

  8. [Rape: a social problem and a public health issue].

    Science.gov (United States)

    Piet, Emmanuelle

    2010-11-01

    The French legal definition of rape was somewhat vague until the 1970s. In December 1980, the increased awareness created by the feminist movement led to a precise legal definition and to the possibility for self-help groups to participate in legal actions. In 1985, a telephone helpline was created after several rapes were following several rapes committed in public. Since 1986, more than 39 000 women have called this hotline. Other recent studies confirm the frequency of rape. Major advances have been made in recent years, in terms of justice for minors who are victims of rape (lengthening of the statutory limitation on sexual crimes) and healthcare provision (opening of refuges, medical-legal consultations, victim management centers, greater awareness among healthcare professionals, etc.). Women need to be better informed of the frequency of rape, its legal implications, rapists' strategies, mental disorders, and the physical (especially gynecological) repercussions of rape. Women must also be aware that the rapist is the only guilty party. Finally, a multidisciplinary strategy is needed, notably involving self-help associations and hospital units that deal with rape victims.

  9. Acquaintance Rape on Campus: The Problem, the Victims, and Prevention.

    Science.gov (United States)

    Neff, Laurie

    1988-01-01

    Asserts that, although providing educational programs and materials on acquaintance rape and prevention strategies is important, attention must also be given to victims. Discusses recognition of stress response pattern of victims suffering from Rape Trauma Syndrome and provision of appropriate referral and support services. Includes selected list…

  10. The emergency management of a rape case in a nutshell ...

    African Journals Online (AJOL)

    The management of a rape case in the emergency department is shrouded in an unacceptable level of lack of confidence. Since South Africa has a high incidence of rape, the contribution of health practitioners in addressing the crime successfully is essential. The medical management of such cases carries a high level of ...

  11. Rape, sexual politics and the construction of manhood among the ...

    African Journals Online (AJOL)

    Pascah Mungwini

    198). Burgess and Holmstrom (1974), in their analysis of the rape trauma syndrome, characterized rape as an act of violence which uses sex as a weapon. As will be highlighted below, the construction of Shona masculinity presents the male in contraposition to the female, thus attributing the active role to the male and the.

  12. Perception of Rape Amongst Secondary School Students In Port ...

    African Journals Online (AJOL)

    Alasia Datonye

    Background: Rape in children is a significant public health problem and represents the least reported but most devastating major form of child abuse all over the world. It occurs when one person does not fully and freely consent to sexual intercourse. This study seeks to evaluate the perception of rape among secondary ...

  13. Attitudes toward rape among Nigerian young adults: the role of ...

    African Journals Online (AJOL)

    Three hypotheses were tested with the independent group t-test. Attitudes of young adults toward rape was found to be different based on gender and parental family structure; male young adults had more positive attitudes toward rape than their female counterparts (t = 2.02, df = 318, p < .05); youth from polygamous family ...

  14. Psycho-legal challenges facing the mentally retarded rape victim

    African Journals Online (AJOL)

    2011-09-03

    Sep 3, 2011 ... against women and children is rife in South Africa, the country has been branded the 'rape capital of the world'. ... the criminal justice system, have regarded people with mental retardation as unreliable witnesses. ..... Another important role player in this legal battle is the police. If a rape survivor with mental ...

  15. The Influence of Rape Myth Acceptance and Situational Factors in ...

    African Journals Online (AJOL)

    Through mixed-method victimisation surveys that covered four universities in Lagos and Ogun states, involving 206 respondents and 12 in-depth interviewees, the study found that emotions and societal perception of rape are strong factors in determining personal conception and description of rape-like experiences.

  16. Suspects in criminal investigations of rape

    Directory of Open Access Journals (Sweden)

    Marinković Darko

    2014-01-01

    Full Text Available Investigations of sexual assaults mostly focus on victims and their credibility, which may cause lack of firm evidence in relation to suspects. Given the fact that the criminal offence of rape is characterised by a high incidence of false reports and accusations, frequently indicating specific persons as the perpetrators, certain caution is necessary in the investigation in order to avoid false accusations and/or convictions. As regards the personality of the rapist and motives for committing a forcible sexual act, certain types or rather certain categories of perpetrators can be distinguished, although it should be noted that a large number of rapists do not belong to one category only, but rather combine characteristics of several different types. During a criminal investigation it is of vital importance to differentiate between a rape as a surprise attack and a rape as abuse of trust, as they are compatible with the nature of the suspect's defence. The suspect shall be subjected to a forensic examination in the course of the investigation in order to find traces which prove vaginal, anal or oral penetration, coerced sexual intercourse and identity of the rapist. While conducting an interrogation of a suspected rapist, a crime investigating officer shall use either factual or emotional approach to his interviewee, depending on his psychological and motivational characteristics. In this regard, the factual approach is believed to be more efficient with anger rapists and sadistic rapists, whereas the compassionate approach gives good results with the gentlemen-rapists and partly with the power asserting rapists.

  17. Ascorbate peroxidase. A prominent membrane protein in oilseed glyoxysomes.

    Science.gov (United States)

    Bunkelmann, J R; Trelease, R N

    1996-02-01

    The glyoxysomes of growing oilseed seedlings produce H2O2, a reactive oxygen species, during the beta-oxidation of lipids stored in the cotyledons. An expression library of dark-grown cotton (Gossypium hirsutm L.) cotyledons was screened with antibodies that recognized a 31-kD glyoxysomal membrane polypeptide. A full-length cDNA clone (1258 bp) was isolated that encodes a 32-kD subunit of ascorbate peroxidase (APX) with a single, putative membrane-spanning region near the C-terminal end of the polypeptide. Internal amino acid sequence analysis of the cotton 31-kD polypeptide verified that this clone encoded this protein. This enzyme, designated gmAPX, was immunocytochemically and enzymatically localized to the glyoxysomal membrane in cotton cotyledons. The activity of monodehydroascorbate reductase, a protein that reduces monodehydroascorbate to ascorbate with NADH, also was detected in these membranes. The co-localization of gmAPX and monodehydroascorbate reductase within the glyoxysomal membrane likely reflects an essential pathway for scavenging reactive oxygen species and also provides a mechanism to regenerate NAD+ for the continued operation of the glyoxylate cycle and beta-oxidation of fatty acids. Immunological cross-reactivity of 30- to 32-kD proteins in glyoxysomal membranes of cucumber, sunflower, castor bean, and cotton indicate that gmAPX is common among oilseed species.

  18. Giving birth with rape in one's past: a qualitative study.

    Science.gov (United States)

    Halvorsen, Lotta; Nerum, Hilde; Oian, Pål; Sørlie, Tore

    2013-09-01

    Rape is one of the most traumatizing violations a woman can be subjected to, and leads to extensive health problems, predominantly psychological ones. A large proportion of women develop a form of posttraumatic stress termed Rape Trauma Syndrome. A previous study by our research group has shown that women with a history of rape far more often had an operative delivery in their first birth and those who gave birth vaginally had second stages twice as long as women with no history of sexual assault. The aim of this study is to examine and illuminate how women previously subjected to rape experience giving birth for the first time and their advice on the kind of birth care they regard as good for women with a history of rape. A semi-structured interview with 10 women, who had been exposed to rape before their first childbirth. Data on the birth experience were analyzed by qualitative content analysis. The main theme was "being back in the rape" with two categories: "reactivation of the rape during labor," with subcategories "struggle," "surrender," and "escape" and "re-traumatization after birth," with the subcategories "objectified," "dirtied," and "alienated body." A rape trauma can be reactivated during the first childbirth regardless of mode of delivery. After birth, the women found themselves re-traumatized with the feeling of being dirtied, alienated, and reduced to just a body that another body is to come out of. Birth attendants should acknowledge that the common measures and procedures used during normal birth or cesarean section can contribute to a reactivation of the rape trauma. © 2013, Copyright the Authors Journal compilation © 2013, Wiley Periodicals, Inc.

  19. Late Adolescents' Perspectives on Marital Rape: The Impact of Gender and Fraternity/Sorority Membership.

    Science.gov (United States)

    Auster, Carol J.; Leone, Janel M.

    2001-01-01

    Examined attitudes toward marital rape-in particular, the impact of gender and fraternity/sorority membership on respondents' views regarding marital rape versus rape by a stranger, as well as feelings about actions that a victim of marital rape may take. While fraternity and nonfraternity men showed significant differences in responses, sorority…

  20. Date Rape: Its Relationship to Trauma Symptoms and Sexual Self-Esteem.

    Science.gov (United States)

    Shapiro, Brenda L.; Schwarz, J. Conrad

    1997-01-01

    Extends previous research on date rape by assessing trauma symptoms and sexual self-esteem among college women who had, or who had not, been raped. Results indicate that rape victims had significantly more trauma symptoms and lower sexual self-esteem compared to other women, thus suggesting date rape's significant consequences. (RJM)

  1. Rape-accepting attitudes of university undergraduate students.

    Science.gov (United States)

    Talbot, Kimberly K; Neill, Karen S; Rankin, Linda L

    2010-01-01

    The College Date Rape Attitudes Survey and the Attitudes Toward Women Scale were used in a cross-sectional study to assess rape-accepting attitudes of a convenience sample of 1602 university undergraduate students using a survey distributed online. The findings evidenced that males and individuals with more traditional gender role beliefs had attitudes more accepting of rape than the females and individuals who had more egalitarian gender role beliefs. Respondents who personally knew a rape survivor had attitudes less accepting of rape than those respondents who did not know a survivor. These findings support a continuing need to address rape myths in sexual violence prevention programming. The sexual assault nurse examiner (SANE-A) has an important role in prevention services to educate and build awareness of rape myths both on campus and through community-based efforts to reach this high risk population. Further, the SANE can build important linkages between the college campus and the healthcare setting to support the provision of effective intervention services and improved outcomes in victims of sexual violence. © 2010 International Association of Forensic Nurses.

  2. Genocide Rape Trauma Management: An Integrated Framework for Supporting Survivors.

    Science.gov (United States)

    Mukamana, Donatilla; Brysiewicz, Petra; Collins, Anthony; Rosa, William

    2017-06-13

    During the 1994 Rwandan genocide, rape was widely used as a strategic weapon against Tutsi women. More than 20 years later, many of these women are still suffering devastating psychological, social, and physical effects of these experiences and remain in need of effective interventions that address their complex trauma. This article develops the theory of genocide rape trauma management as a conceptual framework for promoting the holistic health and recovery of female genocide rape survivors. A qualitative study using grounded theory explored the lived experiences of genocide rape survivors and led to the development of this model. The need for a contextually appropriate model is highlighted, with historical and current data regarding the Rwandan context and the experiences of genocide rape survivors. The research details the complex dynamics of emotional, physical, existential, and psychosocial sequelae related to genocide rape trauma, and how these interact with both local community and broader political attitudes toward survivors, as well as the serious interrelated economic challenges and limitations in current public health and welfare services. The research demonstrates the necessary elements of a holistic approach encompassing a synergistic combination of skilled psychological care, self-help strategies, collaborative support groups, community education, social reintegration, advocacy, accessible medical care, and economic empowerment. These interacting elements form the basis of the theory of genocide rape trauma management, offering an encompassing integrated framework that can be adapted to, and evaluated in, other similar contexts.

  3. ["Biastophilia"--rape as a form of paraphilia?].

    Science.gov (United States)

    Knecht, Thomas

    2014-01-01

    Rape is primarily regarded as a crime against sexual integrity. If rape is the only way for an individual to get sexual satisfaction, it meets the criteria of paraphilia, i.e. a disorder of sexual preference. The correct technical term is "biastophilia". The author investigates the evolutionary origins of this deviant form of copulation behavior, whose crucial characteristic is breaking the will of the sexual object. He shows that in a primeval environment this type of mating behavior may have been a promising strategy to pass on one's genes to the next generation. Thus, the behavioral pattern of raping had the chance to become widely spread in spite of its social undesirability.

  4. Gender-Blind Sexism and Rape Myth Acceptance.

    Science.gov (United States)

    Stoll, Laurie Cooper; Lilley, Terry Glenn; Pinter, Kelly

    2017-01-01

    The purpose of this article is to explore whether gender-blind sexism, as an extension of Bonilla-Silva's racialized social system theory, is an appropriate theoretical framework for understanding the creation and continued prevalence of rape myth acceptance. Specifically, we hypothesize that individuals who hold attitudes consistent with the frames of gender-blind sexism are more likely to accept common rape myths. Data for this article come from an online survey administered to the entire undergraduate student body at a large Midwestern institution (N = 1,401). Regression analysis showed strong support for the effects of gender-blind sexism on rape myth acceptance. © The Author(s) 2016.

  5. Rape in South Africa: an invisible part of apartheid's legacy.

    Science.gov (United States)

    Armstrong, S

    1994-06-01

    Male dominated power systems in South Africa coupled with a culture of aggression and domination have not allowed for the mainstream emergence of qualities such as trust, compassion, and gentleness, frequently associated with female virtues. Rape under such circumstances is the assertion of male power, and, as expected, the numbers of rape cases and other forms of gender violence have increased. Under an apartheid system, where only the rape of white women was prosecuted, and gender and race issues could diffuse the black struggle, there were difficulties in mobilizing support to end violence against black women. There was social acceptance that rape of black women was part of life. Police statistics on the incidence of rape were notoriously unreliable, because only a small percentage of cases are reported to police, and many women would be ashamed to admit to marital abuse, including rape. In women's groups, it was reported that 1 out of every 4 women had been raped, and raped at early ages, such as 14 years of age. In impoverished communities, there is widespread poverty, injustice, prejudice, alcohol abuse, and other conditions impacting on women. The rapists may be school classmates, or street fighters, or political activists. The sexual initiation of women worldwide and in South Africa frequently is accompanied by coercion. Abduction and forced sex is considered legitimate for young men, who believe that mental health is deleteriously affected by lack of sex. Girls may even be abducted from the classroom, as a way of preventing women's power and control over assets and resources and diminishing the potential threat to gender power relations. School girls who become pregnant are often forced to leave school. Victims of rape may be excluded from family life and forced to seek prostitution as a means of support. Rape is reportedly experienced among older women with bottles, tins, or other devices which inflict injury. Only rape with penal penetration is an

  6. Rape trauma syndrome: time to open the floodgates?

    Science.gov (United States)

    Keogh, Andrew

    2007-05-01

    The conviction rate in rape cases remains remarkably low, leading many to believe a new approach is needed to prosecuting such crimes. Many important steps have been taken in relation to the collection and retention of evidence and the setting up of specialist rape prosecutors, but important evidential weaknesses remain present in the system alongside Victorian attitudes to sexual conduct. The British Home Office is currently consulting on a number of measures, including the admissibility of evidence showing the presence of rape trauma syndrome. This paper examines the history of this controversial evidential debate.

  7. Changing attitudes and perceptions of Hispanic men ages 18 to 25 about rape and rape prevention.

    Science.gov (United States)

    Lawson, Sarah Lynn; Munoz-Rojas, Derby; Gutman, Lauren Samantha; Siman, Matilde Nathalia

    2012-12-01

    This exploratory study was designed to test the effectiveness of Foubert's intervention, The Men's Program, on a sample of 18- to 25-year-old Hispanic men who are not enrolled at a University or College. We explored participants' perceptions of, reactions to, and experience of the program. The information collected will be used to adapt the program to this specific population in order to increase cultural relevancy and specificity and create lasting attitudinal change. Three groups of six-eight Hispanic males (n = 22) were exposed to the intervention program. The Bystander Attitude Scale and the Rape Attitude and Beliefs Scale were administered as pre- and post-test measures. A short focus group was conducted to ask the men about their experience of the intervention. Analysis showed a significant increase in participants' willingness to intervene (p = 0.005) along with a decrease in rape myth acceptance in four of the five subscales (Justice p = 0.03; Status p = 0.004; Tactics p = 0.04; and Gender p = 0.002) after exposure to the intervention. Analysis of focus group material yielded several interesting themes about knowledge of rape, family and culture, perceptions of women, and the program material. This study showed promising change in attitudes about rape beliefs and bystander behaviors in Hispanic males exposed to an educational intervention. Through the information obtained, a cultural adaption from the analysis of the focus group data will be implemented during Phase 2 of the study. The adapted intervention will be tested before, after, and 1- and 3-months post-intervention to test whether the change in attitudes and behaviors are sustainable over time.

  8. Draft genome sequence of the oilseed species Ricinus communis.

    Science.gov (United States)

    Chan, Agnes P; Crabtree, Jonathan; Zhao, Qi; Lorenzi, Hernan; Orvis, Joshua; Puiu, Daniela; Melake-Berhan, Admasu; Jones, Kristine M; Redman, Julia; Chen, Grace; Cahoon, Edgar B; Gedil, Melaku; Stanke, Mario; Haas, Brian J; Wortman, Jennifer R; Fraser-Liggett, Claire M; Ravel, Jacques; Rabinowicz, Pablo D

    2010-09-01

    Castor bean (Ricinus communis) is an oilseed crop that belongs to the spurge (Euphorbiaceae) family, which comprises approximately 6,300 species that include cassava (Manihot esculenta), rubber tree (Hevea brasiliensis) and physic nut (Jatropha curcas). It is primarily of economic interest as a source of castor oil, used for the production of high-quality lubricants because of its high proportion of the unusual fatty acid ricinoleic acid. However, castor bean genomics is also relevant to biosecurity as the seeds contain high levels of ricin, a highly toxic, ribosome-inactivating protein. Here we report the draft genome sequence of castor bean (4.6-fold coverage), the first for a member of the Euphorbiaceae. Whereas most of the key genes involved in oil synthesis and turnover are single copy, the number of members of the ricin gene family is larger than previously thought. Comparative genomics analysis suggests the presence of an ancient hexaploidization event that is conserved across the dicotyledonous lineage.

  9. Production of mono- and sesquiterpenes in Camelina sativa oilseed.

    Science.gov (United States)

    Augustin, Jörg M; Higashi, Yasuhiro; Feng, Xiaohong; Kutchan, Toni M

    2015-09-01

    Camelina was bioengineered to accumulate (4 S )-limonene and (+)-δ-cadinene in seed. Plastidic localization of the recombinant enzymes resulted in higher yields than cytosolic localization. Overexpressing 1-deoxy- d -xylulose-5-phosphate synthase ( DXS ) further increased terpene accumulation. Many plant-derived compounds of high value for industrial or pharmaceutical applications originate from plant species that are not amenable to cultivation. Biotechnological production in low-input organisms is an attractive alternative. Several microbes are well established as biotechnological production platforms; however, their growth requires fermentation units, energy input, and nutrients. Plant-based production systems potentially allow the generation of high-value compounds on arable land with minimal input. Here we explore whether Camelina sativa (camelina), an emerging low-input non-foodstuff Brassicaceae oilseed crop grown on marginal lands or as a rotation crop on fallow land, can successfully be refactored to produce and store novel compounds in seed. As proof-of-concept, we use the cyclic monoterpene hydrocarbon (4S)-limonene and the bicyclic sesquiterpene hydrocarbon (+)-δ-cadinene, which have potential biofuel and industrial solvent applications. Post-translational translocation of the recombinant enzymes to the plastid with concurrent overexpression of 1-deoxy-D-xylulose-5-phosphate synthase (DXS) resulted in the accumulation of (4S)-limonene and (+)-δ-cadinene up to 7 mg g(-1) seed and 5 mg g(-1) seed, respectively. This study presents the framework for rapid engineering of camelina oilseed production platforms for terpene-based high-value compounds.

  10. Media Re-victimization of Rape Victims in a Shame Culture? Exploring the Framing and Representation of Rape Cases in Nigerian Dailies

    National Research Council Canada - National Science Library

    Chinenye Nwabueze; Fidelis Oduah

    2015-01-01

      [...]India as a country has in recent time, continued to have its doses of not just rape cases but "gang raping" of powerless, defenseless and innocent women and girls by rapists and "the situation...

  11. Rape embryogenesis. V. Accumulation of lipid bodies

    Directory of Open Access Journals (Sweden)

    Teresa Tykarska

    2014-01-01

    Full Text Available In embryo of winter rape var. Górczański lipid bodies have been observed in the light microscope starting from two-celled proembryo. Their number and size increase in the course of embryogenesis, especially since greening of endosperm and embryo. Lipid bodies, either single or in clusters, are present in all embryo cells, the clusters occur in various parts of the cytoplasm. During embryo maturation lipid bodies move and locate in a number of layers by the cell wall. At the same time their appearance change. Typical storage lipids originate. Lipid bodies are unevenly distributed within the embryo: their number and size decrease along the embryo axis from its part below cotyledons towards root apex. Moreover, they are histologically diversified: the biggest are located in epidermis and cortex, whereas the smallest -in central cylinder. In columella there are fewest lipid bodies.

  12. Rape embryogenesis. III. Embryo development in time

    Directory of Open Access Journals (Sweden)

    Teresa Tykarska

    2014-01-01

    Full Text Available It was found that the growth curve of the rape embryo axis is of triple sigmoid type. Embryo growth occurs in 3 phases corresponding to 3 different periods of development. Phase I includes growth of the apical cell up to it's division into two layers of octants. Phase II comprises the increase of the spherical proembryo to the change of its symmetry from radial to bilateral. Phase III includes, growth of the embryo from the heart stage up to the end of embryogenesis. In each phase the relative growth rate increases drastically and then diminishes. The differences in growth intensity during the same phase are several-fold. The growth intensity maximum of the embryo axis occurs in phase II. The phasic growth intensity maxima occur: in phase I during apical cell elongation, :before its division, and in phases II and III in the periods of cell division ;growth in globular and torpedo-shaped -shaped embryos.

  13. [Anxiety and depression levels in rape offenders].

    Science.gov (United States)

    Herrera-Hernández, E; Marván, L; Saavedra, M; Contreras, C M

    1993-03-01

    Whether the active subject of rape possesses a particular personality trait is a much debated matter. It is an accepted fact that a small amount of rapists fall into the sadistic diagnostic category. Other psychopatologic traits likely to identify a rapist are, however, unknown. The present study was carried over at a jail near the city of Xalapa (Veracruz) Mexico. Hamilton, IDARE, and Zung scales, in their modified Spanish version were applied to a sample of imprisoned adult rapists. Compared to their control group (men serving sentences for other felonies), rapists scored higher in anxiety, and depression scales. In the light of these results, it is suggested that some rapists are likely to be anxious-depressive subjects that, after having "acquired" a sociopathic repertoire, offend other people in conditions of indefensiveness, contrary to depressive subjects who aggress themselves.

  14. Rape embryogenesis I. The proembryo development

    Directory of Open Access Journals (Sweden)

    Teresa Tykarska

    2015-01-01

    Full Text Available The development of the proembryo of rape Brassica napus L. from the zygote to the young embryo proper is described. A number of regularities were found in the direction, succession, and distribution of segmental and differentiating divisions of the proembryo. The direction of the divisions seems to foe determined by the direction of growth and the shape of the cells. The termyoung embryo proper is proposed to denote the globular embryo which already possesses separate plerome and periblem mother-cells and mother-cells of the iec layer and of clumella. The body of the embryo proper is derived from the apical cell ca which arose from the first division of the zygote and from the hypophysis - the only suspensor cell which closes the spheroid of the embryo. The development of the Brassica napus L. proembryo follows the sub-archetype Capsella bursa-pastoris in the IV megarchetype of Soueges.

  15. Fermentation, Digestion Kinetics and Methane Production of Oilseed Press Cakes from Biodiesel Production

    Directory of Open Access Journals (Sweden)

    S. M. Olivares-Palma

    2013-08-01

    Full Text Available Following the extraction of oil for biodiesel production, oilseed press cakes are high in fat. As the dietary supplementation of fat is currently considered the most promising strategy of consistently depressing methanogenesis, it follows that oilseed press cakes may have a similar potential for CH4 abatement. As such, this study aimed to characterise the nutritive value of several oilseed press cakes, glycerine and soybean meal (SBM and to examine their effects on in vitro ruminal fermentation, digestion kinetics and CH4 production. Moringa press oil seeds exhibited the greatest in sacco effective degradability (ED of DM and CP (p<0.05. In vitro gas production (ml/g digested DM was not affected (p = 0.70 by supplement at 48 h of incubation. In vitro DMD was increased with the supplementation of glycerine and SBM at all levels of inclusion. Moringa oilseed press cakes produced the lowest CH4 (mg/g digested DM at 6 and 12 h of incubation (p<0.05. The findings suggest that moringa oilseed press cake at 400 g/kg DM has the greatest potential of the oilseed press cakes examined in this study, to reduce CH4 production, without adversely affecting nutrient degradability.

  16. Evolution of Rape Myths and Sexual Assaults in Newspaper Coverage

    Directory of Open Access Journals (Sweden)

    Yan-Wen Tsai

    2016-07-01

    Full Text Available The article presents an analysis of the coverage of three newspapers of rape in the daily Press in Taiwan. The idea that rape is a sexual rather than an aggressive act encourages people not to take it seriously as a crime-an attitude frequently revealed in comments by defense attorneys and newspaper. The authors’ investigated that the female victim who did not know most of her attackers will be portrayed as helpless and not responsible for her victimization. The newspapers sustained and reinforced the myths that a woman who is having consensual sex cannot be raped, and if so, she is held culpable and perceived as “asking for it.” A content analysis of newspapers’ headlines and coverage between 2002 and 2013 showed that more than 50% endorsed a rape myth.

  17. Party Rape, Nonconsensual Sex, and Affirmative Consent Policies

    National Research Council Canada - National Science Library

    Kelly Oliver

    2015-01-01

    ... of communication, the communication of pleasure. [...]given the increasingly slippery slope between sex and sexual assault, and uncertainty about what counts as rape, many women are subject to sexual aggression, even sexual assault, and are not...

  18. ANALISIS KRIMINOLOGIS TERHADAP TINDAK KEJAHATAN PEMERKOSAAN LELAKI (MALE RAPE)

    OpenAIRE

    AKSAN, ARDHIANSYAH

    2014-01-01

    2014 ARDHIANSYAHAKSAN (B111 10105), dengan judul ???Analisis Kriminologis Terhadap Tindak Kejahatan Pemerkosaan Lelaki (Male Rape)???. Di bawah bimbingan bapak Prof. Dr. AndiSofyan, S.H., M.H., sebagai Pembimbing I dan bapak KaisaruddinKamaruddin, S.H., sebagai Pembimbing II. Tujuan dari penelitian ini adalah untuk mengetahui faktor penyebab terjadinya tindak kejahatan pemerkosaan lelaki (male rape) dan upaya yang dapat ...

  19. Religiousness and Rape Myth Acceptance: Risk and Protective Effects.

    Science.gov (United States)

    Ensz, Samantha; Jankowski, Peter J

    2017-03-01

    This study addressed the lack of research simultaneously examining multiple dimensions of religiousness when predicting rape myth acceptance, and extended prior findings of a mediating role for right-wing authoritarianism (i.e., uncritical submission to authority and aggressive attitude toward those who do not conform to social norms) in the association between religiousness and prejudice. The sample consisted of 99 undergraduate and graduate students ( M age = 31.87 years, 66.7% female, 80.82% White, and 93% Christian affiliated) from a religiously affiliated university in the Midwest United States. As hypothesized, dimensions of religiousness exhibited differential associations with rape myth acceptance. Religious motivation characterized by openness and exploration (i.e., quest religiousness) was a significant negative predictor of rape myth acceptance, directly, and indirectly through right-wing authoritarianism. In contrast, rigid adherence to religious beliefs, assumed to be "right" and absolutely true (i.e., religious fundamentalism), and extrinsically motivated religiousness each exhibited a positive association with rape myth acceptance through right-wing authoritarianism. In addition, internally motivated religiousness and religious fundamentalism each moderated the nonlinear effect for quest predicting rape myth acceptance. Findings suggest that uncritical religious and secular submission to external authorities or uncommitted and nonexploring religiousness may have increased the extent to which persons adhered to rape myths, whereas religious exploration was protective. Practical implications center on the need for socioculturally relevant prevention and intervention efforts with religious identifying college students.

  20. Rape-seed oil - a substitute for mineral oil?

    Energy Technology Data Exchange (ETDEWEB)

    Badent, R.; Kist, K.; Schwab, A.J. [University of Karlsruhe (Germany). Institute of Electric Systems and High-Voltage Technology

    1999-07-01

    This paper presents the results obtained from the study on the basic characteristics of rape-seed oil considered to be a substitute of mineral oil in power transformers. The results show that rape- seed oil is usable as an insulating liquid. Furthermore, results are presented concerning the breakdown behaviour in uniform (plane-plane geometry) and non-uniform (rod-plane geometry) fields under impulse conditions with gaps up to 40 mm and voltages up to 650 kV. A sufficient high 50 Hz breakdown voltage is the most important precondition for a medium to be used as an insulator. The dielectric strength of rape-seed oil exceeds more than 50 kV/2,5 mm and consequently fulfils the requirements of the standards. The measurement of the permittivity and tan {delta} at 90{sup o}C yields to 3,18 and 0,015, respectively and is comparable to the values of standard mineral oil. However, there are still some problems to be overcome. Beside the insulating task the liquid dielectric has also a cooling function in power apparatus. Since the dynamic viscosity of rape-seed oil (about 70 mm{sup 2}/s at 40{sup o}C) is higher than that of mineral oil, rape-seed oil has a lower ability for heat conduction. A further problem is the solidification of rape-seed oil below -5{sup o}C. (author)

  1. Women's erotic rape fantasies: an evaluation of theory and research.

    Science.gov (United States)

    Critelli, Joseph W; Bivona, Jenny M

    2008-01-01

    This article is the first systematic review of the research literature on women's rape fantasies. Current research indicates that between 31% and 57% of women have fantasies in which they are forced into sex against their will, and for 9% to 17% of women these are a frequent or favorite fantasy experience. Erotic rape fantasies are paradoxical: they do not appear to make sense. Why would a person have an erotic and pleasurable fantasy about an event that, in real life, would be abhorrent and traumatic? In this article, the major theories of women's rape fantasies are evaluated both rationally and empirically. These theories explain rape fantasies in terms of masochism, sexual blame avoidance, openness to sexuality, sexual desirability, male rape culture, biological predisposition to surrender, sympathetic physiological activation, and adversary transformation. This article evaluates theory and research, makes provisional judgments as to which theories appear to be most viable, and begins the task of theoretical integration to arrive at a more complete and internally consistent explanation for why many women engage in erotic rape fantasies. Methodological critiques and programs for future research are presented throughout.

  2. Mycobiota of rape seeds in Romania. II. Evaluation of potential antagonistic fungi isolated from rape seeds against the main pathogens of rape crop

    Directory of Open Access Journals (Sweden)

    Tatiana-Eugenia Şesan

    2014-06-01

    Full Text Available In vitro relationships between identified seed- and soil-borne fungi from rape samples have been investigated in order to evaluate their antagonistic ability as potential biocontrol agents. The bioproduct obtained from the Trichoderma viride Pers. (strain Td50 has been tested in vivo against the main phytopathogens of rape: Sclerotinia sclerotiorum (Lib. de Bary, Botrytis cinerea Pers., Alternaria spp. and Fusarium spp. in greenhouse at the Laboratory of Mycology and Plant Pathology, Biology Faculty, University of Bucharest – Romania and in the field at the Agricultural Experimental Research-Development Station Caracal (AERDS, Olt district. The T. viride (strain Td50 bioproduct formulated as a powder for the seed treatment has been effective in the protection of rape plantlets against the above mentioned phytopathogens.

  3. Rape and child sexual abuse: what beliefs persist about motives, perpetrators, and survivors?

    Science.gov (United States)

    McGee, Hannah; O'Higgins, Madeleine; Garavan, Rebecca; Conroy, Ronán

    2011-11-01

    Rape myths are prejudicial and stereotyped beliefs about rape which persist in society. They may have a significant impact on those affected by rape as well as the performance of legal and public participants in the justice system. Rape myths may differ over time and within different societies and cultural settings. Awareness of contemporary and local rape myths is necessary if they are to be successfully challenged through public campaigns and other means. This study sought to assess the prevalence of myths concerning rape and sexual abuse in a national population survey.

  4. Flowering dynamics and pollinator visitation of oilseed echium (Echium plantagineum.

    Directory of Open Access Journals (Sweden)

    Carrie A Eberle

    Full Text Available Echium (Echium plantagineum L. is an alternative oilseed crop in summer-wet temperate regions that provides floral resources to pollinators. Its seed oil is rich in omega-3 fatty acids, such as stearidonic acid, which is desired highly by the cosmetic industry. Seeds were sown in field plots over three years in western Minnesota in spring (early-sown or early summer (late-sown, and flower abundance, pollinator visitation, and seed yields were studied. Initial flowering commenced 41 to 55 d after sowing, and anthesis duration (first flowering to harvest was 34 to 70 d. Late sowing dates delayed anthesis, but increased the intensity of visitation by pollinators. Cumulative flower densities ranged from 1 to 4.5 billion ha-1. Flowers attracted numerous honey bees (Apis mellifera L., as many as 35 per minute of observation, which represented about 50% of all insect visitors. Early-sown echium produced seed yields up to 750 kg ha-1, which were 2-29 times higher than those of late-sown echium. Early sowing of echium in Minnesota provides abundant floral resources for pollinators for up to two months and simultaneously produces seed yields whose profits rival those of corn (Zea mays L..

  5. Ireland: child rape case undermines abortion ban.

    Science.gov (United States)

    1992-11-01

    Abortion has been illegal in Ireland since 1861. This position was written into the national Constitution in 1963 and reconfirmed by referendum in 1983. Contraception is also illegal in the country. The pregnancy of a 14-year old adolescent due to an alleged rape, however, has caused many in Ireland to voice their support for abortion in limited circumstances. Approximately 5000 pregnant women go from Ireland to the United Kingdom annually for abortions. This 14-year old youth also planned to make the crossing, but was blocked from leaving by the Irish police and later by an injunction of the Attorney-General. The Irish Supreme Court upheld the injunction even though the young woman was reportedly contemplating suicide. A national outcry ensued with thousands of demonstrators marching in Dublin to demand the availability of information on abortion and that Irish women be allowed to travel whenever and wherever they desire. 66% of respondents to recent public opinion polls favor abortion in certain circumstances. Ultimately, the Irish Supreme Court reversed their stance to allow pregnant Irish women to travel internationally and gave suicidal Irish women the right to abortions. These decisions were made shortly within the time frame needed for the young lady in question to received a legal abortion in the United Kingdom.

  6. "Lucrece this night I must enjoy thee" : a narcissistic reading of The Rape of Lucrece

    National Research Council Canada - National Science Library

    Koketso, Daniel

    2016-01-01

    The circumstances under which acts of rape are committed, and the relationship between power and sexual aggression, may seem to be distinctly modern concerns, yet Shakespeare explores them in The Rape of Lucrece (1594...

  7. “You Have to Confess”: Rape and the Politics of Storytelling

    Directory of Open Access Journals (Sweden)

    Tara Roeder

    2015-11-01

    Full Text Available This article examines the discourse of rape in contemporary culture, paying special attention to the courtroom setting, where rape victims are often required to tell cohesive, linear narratives that underscore their blamelessness if they hope to be believed. Because of deeply entrenched cultural myths about rape, the type of story often required for the successful prosecution of perpetrators may require rape victims to construct narratives that do not accurately reflect their lived experience. Writers such as Susan Brison, Patricia Weaver Francisco, and Alice Sebold engage with the complex politics of rape and its telling in their memoirs. While constructing stories that will suffice in the courtroom setting remains an important task for many rape victims, such stories may ultimately have to be relinquished and rewritten in order to revise prevailing cultural perceptions of rape, its perpetrators, and its victims. The memoirs of rape survivors thus come to function as a different—and necessary—type of public testimony.

  8. Mutilating granuloma inguinale after rape A case report | O'Farrell ...

    African Journals Online (AJOL)

    Abstract. A woman with extensive mutilating lesions of genital granuloma inguinale following rape is described. As far as is known granuloma inguinale has not previously been reported in a rape victim.

  9. Suspect aggression and victim resistance in multiple perpetrator rapes.

    Science.gov (United States)

    Woodhams, Jessica; Cooke, Claire

    2013-11-01

    Several research studies have reported an elevated level of aggression in rapes committed by multiple perpetrators compared to rapes committed by lone suspects. Several factors that have been linked to elevated aggression in generic samples of rape were examined for the first time with a sample of multiple perpetrator rapes. Factors that might be associated with victim resistance were also investigated. Victim and offender characteristics, as well as the behaviors displayed by victims and offenders, were extracted from the police files of 89 multiple perpetrator stranger rapes perpetrated against female victims in the United Kingdom. These behaviors were rated for their level of suspect (non-sexual) aggression and victim resistance, respectively. Degree of victim resistance was significantly and positively associated with suspect aggression. Older victims were the recipients of significantly higher levels of suspect aggression. Victims who were incapacitated from drugs and/or alcohol were less likely to be the recipients of suspect aggression. Group leaders displayed more aggression towards the victim than the followers in the groups. The number of perpetrators was significantly related to the degree of resistance displayed by the victim with offences perpetrated by fewer suspects being characterized by more victim resistance. Research regarding cognitive appraisal during criminal interactions and the respective roles of offenders is referred to in considering these relationships.

  10. “You Have to Confess”: Rape and the Politics of Storytelling

    OpenAIRE

    Tara Roeder

    2015-01-01

    This article examines the discourse of rape in contemporary culture, paying special attention to the courtroom setting, where rape victims are often required to tell cohesive, linear narratives that underscore their blamelessness if they hope to be believed. Because of deeply entrenched cultural myths about rape, the type of story often required for the successful prosecution of perpetrators may require rape victims to construct narratives that do not accurately reflect their lived experience...

  11. Lack of Control as a Predictive Factor for Stress-related Symptoms in Rape Victims

    OpenAIRE

    Sombke, Chad

    1993-01-01

    Researchers have agreed that most rape victims vii experience stress-related symptoms similar to post-traumatic stress disorder. There have also been numerous studies that have tried to predict the severity of those stress-related symptoms, but the literature is inconclusive. Lack of perceived control is consistently mentioned in the rape research literature as being present in rape victims, but no study has empirically examined the relationship between perceived control and a rape victim's s...

  12. Effect of oilseed source on ruminal fermentation and methane production of a grass-legume diet in continuous culture

    Science.gov (United States)

    Addition of oilseeds to pasture-based ruminant diets has been shown to decrease enteric CH4 emissions. However, little research has directly compared the effect of oilseed source on ruminal fermentation and Methane (CH4) production. A 4-unit continuous culture fermentor system was used to test 4 oil...

  13. From Survivor to Thriver: A Pilot Study of an Online Program for Rape Victims

    Science.gov (United States)

    Littleton, Heather; Buck, Katherine; Rosman, Lindsey; Grills-Taquechel, Amie

    2012-01-01

    Approximately 15% to 20% of women have been victims of rape and close to a third report current rape-related PTSD or clinically significant depression or anxiety. Unfortunately, very few distressed rape victims seek formal help. This suggests a need to develop alternative ways to assist the many distressed victims of sexual violence. Online…

  14. Profile of rape victims referred by the court to the Free State ...

    African Journals Online (AJOL)

    Background. The psychological evaluation of rape victims to determine their competency to testify in court and whether they are capable of consenting to sexual intercourse is challenging, especially when the rape victim is mentally retarded. Objective. To describe the profile of mentally retarded rape victims referred to the ...

  15. Sexual Assault Nurse Examiners' Perceptions of Their Relationship with Doctors, Rape Victim Advocates, Police, and Prosecutors

    Science.gov (United States)

    Maier, Shana L.

    2012-01-01

    In response to the negative and inefficient treatment of rape victims by emergency room personnel, the first Sexual Assault Nurse Examiner (SANE) programs began in the late 1970s. While SANEs, doctors, rape victim advocates, police officers and prosecutors work together to ensure the most comprehensive and sensitive care of rape victims, they all…

  16. Rape Perception and the Function of Ambivalent Sexism and Gender-Role Traditionality

    Science.gov (United States)

    Yamawaki, Niwako

    2007-01-01

    This study explores the roles of benevolent sexism (BS), hostile sexism (HS), and gender-role traditionality (GRT) in minimizing rape, blaming the victim, and excusing the rapist. As predicted, hostile sexists minimize the seriousness of the rape in both stranger and date-rape scenarios. In the victim-blame scale, both BS and GRT significantly…

  17. Perceptions of Rape and Attitudes toward Women in a Sample of Lebanese Students

    Science.gov (United States)

    Rebeiz, Maria J.; Harb, Charles

    2010-01-01

    This study investigated values, ambivalent sexism, religiosity, religious differences, gender, and attitudes toward rape victims as predictors of rape myths in a sample of Lebanese students (N = 300). Values of self-transcendence and conservation, gender, hostile sexism, and attitudes toward rape victims emerged as significant predictors of rape…

  18. 75 FR 27000 - Hearings of the Review Panel on Prison Rape

    Science.gov (United States)

    2010-05-13

    ... of Justice Programs Hearings of the Review Panel on Prison Rape AGENCY: Office of Justice Programs... Review Panel on Prison Rape (Panel) will hold hearings in Washington, DC on June 3-4, 2010. The hearing... Prison Rape Elimination Act of 2003, Public Law 108-79, 117 Stat. 972 (codified as amended at 42 U.S.C...

  19. Pilot study to assess the viability of a rape trauma syndrome questionnaire.

    Science.gov (United States)

    Long, F Y; Pang, E; Kee, C

    2002-11-01

    Studies have revealed that rape victims undergo a number of psychological symptoms following the attack, which constitute a specific syndrome termed the rape trauma syndrome (RTS). Evidence of the RTS has been admitted as scientific testimony in the prosecution of sexual offences and has been integral in their successful conviction. The present study aims to assess the viability of a questionnaire designed to identify the RTS in victims of alleged rape. A 77-item rape trauma syndrome questionnaire (RTSQ) was developed and administered to 30 women who reported rape and 57 nurses who formed the control group. The data were analysed using the Statistical Package for the Social Sciences (Windows Version 6.0). Statistical analysis suggested that the questionnaire was internally consistent and effective in uncovering significant differences between rape victims and controls in their experience of rape trauma symptoms. Rape victims scored significantly higher than controls on the RTS scale. Those who faked rape were also found to endorse a greater number of the rape trauma symptoms than actual rape victims, as well as a greater number of fictitious and unlikely symptoms. This pilot study confirmed the viability of the RTSQ and paves the way for a more rigorous examination of its reliability and validity. In the future, the questionnaire may be of use in ascertaining the veracity of victims' claims of rape in the conviction of sexual offences where circumstances are equivocal.

  20. Mycobiota of rape seeds in Romania. I. Identification of mycobiota associated with rape seeds from different areas of Romania

    Directory of Open Access Journals (Sweden)

    Tatiana-Eugenia Şesan

    2013-12-01

    Full Text Available The spectrum of fungal diversity associated with rape seeds belonging to 33 cultivars (Alaska, Astrada, Astrid, Atlantic, Betty, Champlein, Chayenne, Dexter, Digger, Elvis, Eurowest, Finesse, Herkules, Hydromel, Hydromel-MA, Ladoga, anitoba, Masa Rom, Milena, Mohican, Montego, Nectar, Ontario, Orkan, Perla (4 lots, Remy, Robust, Rodeo, Saphir, Tiger, Tiger CBC Lot ROM06-121-110, Triangle, Valesca, Vectra and 2 hybrids (H-90-20-83, H-90-21-83 has been established by samples’ macroscopical and microscopical analizying, during 2006-2008, for the first time in Romania. The Ulster method on malt-agar and PDA culture media has been used, evaluating the percentage of fungal taxons present on/in rape seeds. The most important pathogenic fungi identified were: Sclerotinia sclerotiorum (Lib. de Bary, Botrytis cinerea Pers., Rhizoctonia solani Kühn, Alternaria brassicae (Berk. Sacc., A. brassicicola (Schwein. Wiltshire and Fusarium spp. Also, a large quantities of some saprophytic fungi, as Alternaria, Cladosporium, Aspergillus, Penicillium, Rhizopus have been recorded. These ones have been affected the health condition of rape seeds, suppressing their germination and other vital phenomena. Among potential antagonistic fungi the following genera have been isolated: Chaetomium (0-4%, Trichoderma (0-10%, Aspergillus (0-14%, Penicillium (0-100%. Some correlations and comparisons have been established between fungal diversity, their provenience, cultivars, culture media (Malt-Agar/MA, Potato-Dextrose-Agar/PDA used. It has been evaluated the behaviour of rape cultivars and hybrids towards the main rape seed pathogens.

  1. Fear of rape among college women: a social psychological analysis.

    Science.gov (United States)

    Pryor, Douglas W; Hughes, Marion R

    2013-01-01

    This article examines social psychological underpinnings of fear of rape among college women. We analyze data from a survey of 1,905 female undergraduates to test the influence of 5 subjective perceptions about vulnerability and harm: unique invulnerability, gender risk, defensibility, anticipatory shame, and attribution of injury. We include 3 sources of crime exposure in our models: past sexual victimization, past noncontact violent victimization, and structural risk measured by age, parent's income, and race. Separate measures of fear of stranger and acquaintance rape are modeled, including variables tapping current versus anticipatory fear, fear on campus versus everywhere, and fear anytime versus at night. The data show that fear of rape among college women appears more grounded in constructed perceptions of harm and danger than in past violent experiences.

  2. The Emerging Oilseed Crop Sesamum indicum Enters the "Omics" Era.

    Science.gov (United States)

    Dossa, Komivi; Diouf, Diaga; Wang, Linhai; Wei, Xin; Zhang, Yanxin; Niang, Mareme; Fonceka, Daniel; Yu, Jingyin; Mmadi, Marie A; Yehouessi, Louis W; Liao, Boshou; Zhang, Xiurong; Cisse, Ndiaga

    2017-01-01

    Sesame (Sesamum indicum L.) is one of the oldest oilseed crops widely grown in Africa and Asia for its high-quality nutritional seeds. It is well adapted to harsh environments and constitutes an alternative cash crop for smallholders in developing countries. Despite its economic and nutritional importance, sesame is considered as an orphan crop because it has received very little attention from science. As a consequence, it lags behind the other major oil crops as far as genetic improvement is concerned. In recent years, the scenario has considerably changed with the decoding of the sesame nuclear genome leading to the development of various genomic resources including molecular markers, comprehensive genetic maps, high-quality transcriptome assemblies, web-based functional databases and diverse daft genome sequences. The availability of these tools in association with the discovery of candidate genes and quantitative trait locis for key agronomic traits including high oil content and quality, waterlogging and drought tolerance, disease resistance, cytoplasmic male sterility, high yield, pave the way to the development of some new strategies for sesame genetic improvement. As a result, sesame has graduated from an "orphan crop" to a "genomic resource-rich crop." With the limited research teams working on sesame worldwide, more synergic efforts are needed to integrate these resources in sesame breeding for productivity upsurge, ensuring food security and improved livelihood in developing countries. This review retraces the evolution of sesame research by highlighting the recent advances in the "Omics" area and also critically discusses the future prospects for a further genetic improvement and a better expansion of this crop.

  3. EXPRESSION OF CHITINASE GENE IN TRANSGENIC RAPE PLANTS

    Directory of Open Access Journals (Sweden)

    Lu Longdou

    2005-08-01

    Full Text Available The hypocotyl and cotyledon of Brassica napus L. H165 and Brassica juncea DB3 were transformed with chitinase gene and herbicide-resistance gene by co-culture with Agrobacterium tumefacients LBA4404, and rape plants were obtained which could grow on the medium containing herbicide. The PCR result showed that exotic genes were integrated in the genome of the rape. Further study was performed to determine the impact of temperature on the transgenic rate and the differentiation of explants.

  4. Sexual rape in children and adolescents: a medical emergency

    Directory of Open Access Journals (Sweden)

    García Piña Corina Araceli

    2014-07-01

    Full Text Available Sexual rape is defined as vaginal, anal or oral sex with violent and forceful penetration of the penis or of any other object. Patients who have been raped are a medical emergency which requires immediate attention, if possible, within 24 to 72 hours, since there is the risk of sustaining external and internal injuries and of acquiring a sexually transmitted infection (STI. Detection and Centers for Disease Control (CDC have reported that the maximum effective- ness of HIV prophylaxis is obtained when given within the first 24 to 72 hours post exposure.

  5. Attitude May Be Everything, But Is Everything an Attitude? Cognitive Distortions May Not Be Evaluations of Rape.

    Science.gov (United States)

    Nunes, Kevin L; Hermann, Chantal A; White, Kristen; Pettersen, Cathrine; Bumby, Kurt

    2016-01-18

    Cognitive distortions are often referred to as attitudes toward rape in theory, research, and clinical practice pertaining to sexual aggression. In the social-psychological literature, however, attitudes are typically defined as evaluations; thus, in this context, attitudes toward rape are considered evaluations of rape (e.g., rape is negative vs. positive). The purpose of the current study was to explore whether a widely used measure of cognitive distortions (RAPE Scale; Bumby, 1996) assesses evaluation of rape, and, if not, whether evaluation of rape and the cognitions assessed by the RAPE Scale are independently associated with sexually aggressive behavior. Participants (660 male undergraduate students) completed the RAPE Scale as well as measures of evaluation of rape and sexually aggressive behavior. An exploratory factor analysis revealed that the RAPE Scale items formed a correlated but distinct factor from the Evaluation of Rape Scale items. Regression analyses indicated that the Evaluation of Rape Scale and the RAPE Scale had small to moderate independent associations with self-report measures of sexually aggressive behavior. Our results suggest that evaluation of rape may be distinct from cognitive distortions regarding rape, and both evaluation and cognitive distortions may be relevant for understanding sexual violence. © The Author(s) 2016.

  6. It's Her Fault: Student Acceptance of Rape Myths On Two College Campuses.

    Science.gov (United States)

    Hayes, Rebecca M; Abbott, Rebecca L; Cook, Savannah

    2016-11-01

    The present study examined factors that are associated with an individual's adherence to rape myths at two colleges located in the same town. Particularly, we examined sex, race, and participants' drinking behavior in relation to rape myth acceptance. We found that males and heavy drinkers are more likely than females and non/low drinkers to adhere to rape myths. An interaction between males and drinking was also found indicating a moderated effect of gender on rape myth acceptance. In addition, the college with sexual assault programming did not experience a lowered acceptance of rape myths compared with the college with no programming. © The Author(s) 2016.

  7. Evaluating stranger and acquaintance rape: the role of benevolent sexism in perpetrator blame and recommended sentence length.

    Science.gov (United States)

    Viki, G Tendayi; Abrams, Dominic; Masser, Barbara

    2004-06-01

    In most jurisdictions, the law does not recognize the distinction between stranger and acquaintance rape. However, these two types of rape seem to elicit different responses from both lay observers and legal practitioners. Two studies investigating the role of benevolent sexism (BS) in accounting for participants' responses to acquaintance vs. stranger rape perpetrators are reported. Participants were presented with vignettes describing either an acquaintance rape or a stranger rape. As predicted, relative to low-BS individuals, participants who scored high in BS attributed less blame (Study 1) and recommended shorter sentences (Study 2) for the acquaintance rape perpetrator. Benevolent sexism was unrelated to reactions to the perpetrator in the stranger rape condition.

  8. Visualising Shattered Lives: Potentiality in Representations of Rape Victimisation in Contemporary Japan

    Directory of Open Access Journals (Sweden)

    Hei-Lei Cheng

    2016-07-01

    Full Text Available This article examines how cinematic representations of rape can challenge the silence which surrounds the issue of rape and sexual violence in Japan. A textual analysis of two contemporary fictional Japanese films, DV: Domestic Violence [2005] and The Ravine of Goodbye [2013], was performed to illustrate how filmmakers can use narrative and cinematographic techniques to influence the viewer to reflect upon their attitudes to rape and rape victims. By examining how these two films depict rape and rape recovery, this article argues that there is discursive potential inherent within cinema to shape our imaginations and ideals about the world. But while the filmmaker can construct rape representations that encourage reflection, how the viewer decides to engage with the film has bearing on whether this potential for reflection is realised.

  9. Labeling Sexual Victimization Experiences: The Role of Sexism, Rape Myth Acceptance, and Tolerance for Sexual Harassment.

    Science.gov (United States)

    LeMaire, Kelly L; Oswald, Debra L; Russell, Brenda L

    2016-01-01

    This study investigated whether attitudinal variables, such as benevolent and hostile sexism toward men and women, female rape myth acceptance, and tolerance of sexual harassment are related to women labeling their sexual assault experiences as rape. In a sample of 276 female college students, 71 (25.7%) reported at least one experience that met the operational definition of rape, although only 46.5% of those women labeled the experience "rape." Benevolent sexism, tolerance of sexual harassment, and rape myth acceptance, but not hostile sexism, significantly predicted labeling of previous sexual assault experiences by the victims. Specifically, those with more benevolent sexist attitudes toward both men and women, greater rape myth acceptance, and more tolerant attitudes of sexual harassment were less likely to label their past sexual assault experience as rape. The results are discussed for their clinical and theoretical implications.

  10. National Comparisons of Rape Myth Acceptance Predictors Between Nonathletes and Athletes From Multi-Institutional Settings.

    Science.gov (United States)

    Navarro, John C; Tewksbury, Richard

    2017-09-01

    Athletes are cited as common perpetrators of sexual victimization and are at greater risk of becoming offenders compared with nonathletes. Demographic, lifestyle, and social characteristics of 624 nonathletes and 101 athletes from 21 U.S. Division I postsecondary educational institutions were assessed, with the updated Illinois Rape Myth Acceptance scale gauging endorsement of rape myths. Results indicate that athletes and nonathletes were similar in the degree of rape myth acceptance, with athletes reporting stronger agreement with rape myths than nonathletes did. Predictors of rape myth acceptance among nonathletes are multidimensional. In contrast, nondemographic characteristics like Greek membership, the number of alcoholic beverages consumed, and knowing a sexual assault victim predict rape myth acceptance for athletes, with gender not predicting rape myth acceptance nor different between genders of athletes. Policy implications and future research are discussed.

  11. Pornography, Sexual Callousness, and the Trivialization of Rape.

    Science.gov (United States)

    Zillmann, Dolf; Bryant, Jennings

    1982-01-01

    Explored the consequences of continued exposure to pornography on beliefs about sexuality in general and on dispositions toward women in particular. Found that massive exposure to pornography resulted in a loss of compassion toward women as rape victims and toward women in general. (PD)

  12. "CORRECTIVE RAPE" OF LESBIANS IN THE ERA OF ...

    African Journals Online (AJOL)

    NWUuser

    Albertyn C "Gendered Transformation in South African Jurisprudence: Poor. Women and the Constitutional Court" 2011 Stell LR 591-613. Anguita 2012 Int'l J Hum Rts. Anguita LA "Tackling Corrective Rape in South Africa: The Engagement between the LGBT CSO's and the NHRI's (CGE and SAHRC) and its Role" 2012.

  13. Genotypic variation of rape in phosphorus uptake from sparingly ...

    African Journals Online (AJOL)

    4H2O) and Al-P (AlPO4), were investigated to elucidate the contributions of root morphology and organic acids exudation to P uptake by rape from iron phosphate and aluminum phosphate. By solution culture and sand culture experiments, the ...

  14. The relationshp between rape experience and post-traumatic stress ...

    African Journals Online (AJOL)

    This study was carried out to find out the relationship between rape experience and of Post-traumatic Stress Disorder among victims. One hundred and thirty-two female participants made up of seventy-two from Nasarawa State University and sixty from the general public in Keffi town of Nasarawa State participated in the ...

  15. Event characteristics and socio-demographic features of rape ...

    African Journals Online (AJOL)

    Objectives: On account of increasing awareness of the need for Post exposure prophylaxis (PEP) and availability of requisite drugs, victims of rape are now presenting at health facilities including ours to access PEP for HIV. This study set to document the socio-demographic features of these victims and the event ...

  16. Psychological Techniques In Helping Rape Victims | Ayinde | Edo ...

    African Journals Online (AJOL)

    Counselling psychology is concerned with assisting people who have personal, social, educational and vocational problems to better understand themselves and solve their problems. Rape is seen as a problem that militates against the psychological well being of victims, because of the devastating aftermath of the incident ...

  17. Constructing victims and perpetrators of rape in the advice column

    African Journals Online (AJOL)

    Kate H

    Abstract. This article reports on the ways in which the rape of women by men is constructed in the advice column Dear Dolly, published in the South African periodical Drum Magazine. The data collected for the study spans from 1984 to 2004, encompassing both 10 years before and 10 years after the onset of democracy in ...

  18. Forcible Rape: A Literature Review and Annotated Bibliography.

    Science.gov (United States)

    Chappell, Duncan; Fogarty, Faith

    This selective, annotated bibliography provides guidance to the source and content of a small proportion of the existing publicity about rape, Non-English-language publications, as well as those receiving only limited distribution, are excluded. All materials are recent, most having been published during the 1970's. Outdated materials, such as…

  19. Alcohol-Involved Rapes: Are They More Violent?

    Science.gov (United States)

    Abbey, Antonia; Clinton, A. Monique; McAuslan, Pam; Zawacki, Tina; Buck, Philip O.

    2002-01-01

    Alcohol's psychological, cognitive, and motor effects contribute to rape. Based on theory and past research, we hypothesized that there would be a curvilinear relationship between the quantity of alcohol consumed by perpetrators and how aggressively they behaved. Moderate levels of intoxication encourage aggressiveness; however, extreme levels…

  20. Trichomonas vaginalis among women raped in Antananarivo, 2005-2011.

    Science.gov (United States)

    Andrianarivelo Andry, M; Raherinaivo Anjatiana, A; Razafindrakoto Ainamalala, C; Rasoanandrasana, S; Ravaoarisaina Zakasoa, M; Rasamindrakotroka, A

    2017-02-01

    Rape is a forensic emergency due to the possibility of sexually transmitted infections, including HIV/AIDS. Moreover, an upsurge is currently underway. The objective of this study was to determine the incidence of Trichomonas vaginalis in women undergoing tests at the Microbiology Unit of the Joseph Ravoahangy Andrianavalona Antananarivo Hospital after being raped. This retrospective descriptive study covers the 7-year period from 2005 to 2011 and reports the results of direct examination of the vaginal discharge after vulvar smears from rape patients. We studied the age of patients and the results of these direct smear examinations that tested for T. vaginalis. In our study, the number of women with T. vaginalis increased markedly with age. The mean age of the patients was 13.7 years (range: 2 to 41 years). Among the 232 rape victims tested, only 26 were positive for it (11.20%); trichomoniasis was thus rare among these women. T. vaginalis is a common cause of vaginitis in women and one of the most common infections transmitted by sexual assault. In children, the presence of T. vaginalis in vaginal sample is a strong indicator of sexual abuse. Other sexually transmitted infections can be transmitted during sexual abuse.

  1. Rape and Adultery in Ancient Greek and Yoruba Societies | Olasope ...

    African Journals Online (AJOL)

    In Athens and other ancient cultures, a woman, whatever her status and whatever her age or social class, was, in law, a perpetual minor. Throughout her life, she was in the legal control of a guardian who represented her in law. Rape, as unlawful carnal knowledge of a woman, warranted a capital charge in the ...

  2. Perceptions Matter: Case Studies of Policing Statutory Rape

    Science.gov (United States)

    Kandakai, Tina L.; Ding, Kele; Broomfield, Tyree S.; Iverson, Susan V.

    2013-01-01

    The legal parameters regarding adult-child sexual intimacy varies across states. In many states, rules regarding the definition of "child" and age-of-consent laws seem to perpetuate lax and ambivalent responses to adult- child sex. To explore the criminal response to adult-child sex, particularly statutory rape, seven active male and…

  3. Knowledge and Perceptions of Date Rape among Female ...

    African Journals Online (AJOL)

    AJRH Managing Editor

    in, or may result in, physical, sexual or psychological harm or ... normalization of sexual coercion in intimate relationships8. Sometimes even .... Vaginal expansion. 6. 1.2. Psychological health effects of date rape. PTSD. 151. 29.5. Depression. 125. 25.6. Low self esteem. 57. 11.6. Fear of having sex. 42. 8.6. Shame. 22. 4.5.

  4. Rape, sexual politics and the construction of manhood among the ...

    African Journals Online (AJOL)

    This paper interrogates the language that mediates sex and sexuality among the Shona of Zimbabwe. It draws from the method of ordinary language philosophy to argue that culture, and specifically language, can constitute an effective incubator for the emotions that result in rape. Further, the paper shows how the ...

  5. Centering: reducing rape trauma syndrome anxiety during a gynecologic examination.

    Science.gov (United States)

    Dole, P J

    1996-10-01

    1. Individuals often delay seeking routine gynecologic care or have difficulty completing gynecologic examinations due to the effects of trauma. 2. Advance practice nurses (APN) are challenged to participate with clients to reduce their anxiety, thereby decreasing the health risks associated with avoiding routine gynecologic care. 3. "Centering" is a holistic, noninvasive modality to reduce anxiety associated with rape trauma syndrome.

  6. Maps of Woe Narratives of Rape in Early Modern England

    Directory of Open Access Journals (Sweden)

    Donatella Pallotti

    2013-03-01

    Full Text Available By considering a selection of texts, both fictional and non-fictional, this study ad- dresses different representations of rape in early modern English culture. Its aim is to highlight the interconnections between aspects of culture and the creative exchange, the confrontation and mutual assimilation between ‘high’ and ‘low’ cultural forms.

  7. Bioconversion of rape straw into a nutritionally enriched substrate by ...

    African Journals Online (AJOL)

    ... (Saccharomyces cerevisiae, Candida tropicalis and Candida utilis), into an enriched substrate with increased crude protein and digestibility. Orthogonal experiment showed that the optimal experimental condition for the crude protein enrichment was: 10% (v/w) C. utilis inoculum was added to the rape straw medium after ...

  8. The Experience of Male Rape in Non-Institutionalised Settings ...

    African Journals Online (AJOL)

    ... the effects of the assault on the self, disclosure, the learning and life changes brought about by the assault, the victims' feelings towards their assailants and the effect of the assault on the victims' relationships. This study hopes to facilitate further descriptive research on the phenomenon of male rape in order that greater ...

  9. Sexual Coercion on Dates: It's Not Just Rape.

    Science.gov (United States)

    Waldner-Haugrud, Lisa

    1995-01-01

    Contends that sexual coercion while dating falls under the umbrella of school-related violence. Discusses acquaintance and date rape, a sexual coercion continuum, and coercion victims. Concludes with a discussion of six techniques to reduce risk of sexual coercion. (CFR)

  10. Perception of Spousal Rape among Lagos State University ...

    African Journals Online (AJOL)

    The recognition of sex in the context of marriage as crime is strange. Men are often accused of exclusively perpetrating this culturally alien offence which exclusively causes enormous trauma to women within the family. This study examines the perception of undergraduates about marital rape among married couples in ...

  11. Clinical Perspective Psycho-legal issues surrounding the rape of ...

    African Journals Online (AJOL)

    The rape of individuals with mental retardation poses challenges for the judicial system as well as for the survivors themselves. As a result, the courts tend to refer these individuals for forensic mental health examination to assist them in proceeding with criminal trials. This sequence of events may appear unusual, ...

  12. Why Rape Survivors Participate in the Criminal Justice System

    Science.gov (United States)

    Patterson, Debra; Campbell, Rebecca

    2010-01-01

    After a rape, survivors may seek help from multiple community organizations including the criminal justice system (CJS). Research has found that few survivors report their assaults to the police and of those who do report, many withdraw their participation during the investigation. However, relatively little is known about the factors that lead…

  13. The lived experience of genocide rape survivors in Rwanda.

    Science.gov (United States)

    Mukamana, Donatilla; Brysiewicz, Petra

    2008-01-01

    The purpose of this study was to explore the lived experience of women who were raped during the 1994 genocide in Rwanda. A phenomenological approach was used and this study was carried out in Rwanda in three different locations. The 7 participants took part in three semi-structured, individual interviews which were audiotaped. Participants reported many themes unique to Rwandan women survivors of genocide. These themes included violation by perceived inferiors, loss of dignity and respect, loss of identity, social isolation, loss of hope for the future (i.e., HIV/AIDS), the ongoing torture of rape babies, and developing a sense of community. Rwandan women survivors of the 1994 genocide have lived through unimaginable suffering. Limited information is available regarding the experiences of these rape survivors and this information could create awareness and some understanding of what these women endured. This study can help nurses to understand the sequelae of war and rape and thus have needed information which can be used to offer assistance to women in these circumstances.

  14. TRANSGENIC PLANTS OF RAPE (BRASSICA NAPUS L. WITH GENE OSMYB4 HAVE INCREASED RESISTANCE TO SALTS OF HEAVY METALS

    Directory of Open Access Journals (Sweden)

    Raldugina G.N.

    2012-08-01

    fact that at very high concentrations of salts non-transformed plants died after 12-13 days, whereas the transgenic oilseed rape remained alive long enough time. Thus, the incorporation of the plant gene transcription factor OSMYB4 increased the resistance of transgenic plants to the stress effect of HM.

  15. Oilseed Radish (Raphanus Sativus) Effects on Soil Structure and Soil Water Relations

    Science.gov (United States)

    Oilseed radish (Raphanus sativus spp. oleifera) reduces nematode populations. Fall-incorporated radish biomass may also improve soil physical and hydraulic properties to increase the yield and quality of subsequently grown sugarbeet (Beta vulgaris L.). This field study determined radish effects on...

  16. Effect of Enzymatic Hydrolysis on the Antioxidant Properties of Alcoholic Extracts of Oilseed Cakes

    Directory of Open Access Journals (Sweden)

    Jacek Arct

    2013-01-01

    Full Text Available The aim of the present study is to compare changes in the total phenolic, flavonoid and reducing sugar content and antioxidant activity of alcoholic extracts of Oenothera biennis, Borago officinalis and Nigella sativa oilseed cakes before and after enzymatic hydrolysis. Extraction with ethanol and hydrolysis with different commercially available glycosidases: α-amylase, β-glucosidase, β-glucanase and their combinations in a ratio of 1:1:1 were investigated. Total phenolic, flavonoid and reducing sugar content, iron-chelating activity and antioxidant activity according to DPPH and ABTS tests were measured in non-hydrolysed extracts and compared with the results obtained for the extracts after the application of immobilised enzymes. As a result, the hydrolysed extracts had a higher phenolic and reducing sugar content as well as higher iron-chelating and antioxidant activities. Total phenolic content of Oenothera biennis, Borago officinalis and Nigella sativa oilseed cake extracts after enzymatic hydrolysis was higher in comparison with non-hydrolysed extracts, i.e. 2 times (for the enzyme combination, and 1.5 and 2 times (for β-glucanase (p<0.05, respectively. The best results in increasing the flavonoid and sugar content as well as in iron-chelating activity were obtained after enzymatic hydrolysis of oilseed cake extracts by β-glucanase. Oilseed cake extracts after hydrolysis with an enzyme combination in a ratio of 1:1:1 had the highest increase in antioxidant activity.

  17. Effect of unconventional oilseeds (safflower, poppy, hemp, camelina) on in vitro ruminal methane production and fermentation.

    Science.gov (United States)

    Wang, Shaopu; Kreuzer, Michael; Braun, Ueli; Schwarm, Angela

    2017-08-01

    Dietary supplementation with oilseeds can reduce methane emission in ruminants, but only a few common seeds have been tested so far. This study tested safflower (Carthamus tinctorius), poppy (Papaver somniferum), hemp (Cannabis sativa), and camelina (Camelina sativa) seeds in vitro using coconut (Cocos nucifera) oil and linseed (Linum usitatissimum) as positive controls. All the tested oilseeds suppressed methane yield (mL g-1 dry matter, up to 21%) compared to the non-supplemented control when provided at 70 g oil kg-1 dry matter, and they were as effective as coconut oil. Safflower and hemp were more effective than linseed (21% and 18% vs. 10%), whereas the effects of poppy and camelina were similar to linseed. When methane was related to digestible organic matter, only hemp and safflower seeds and coconut oil were effective compared to the non-supplemented control (up to 11%). The level of methanogenesis and the ratios of either the n-6:n-3 fatty acids or C18:2 :C18:3 in the seed lipids were not related. Unconventional oilseeds widen the spectrum of oilseeds that can be used in dietary methane mitigation. In vivo confirmation of their methane mitigating effect is still needed, and their effects on animal performance still must be determined. © 2017 Society of Chemical Industry. © 2017 Society of Chemical Industry.

  18. Cultivar and planting date selection for relay-cropping soybean with winter oilseeds

    Science.gov (United States)

    Double- and relay-cropping soybean with winter camelina (Camelina sativa L. Crantz) and pennycress (Thlaspi arvense L.) have been shown to be viable cropping systems for the Upper Midwest. Relaying soybean with these winter oilseeds can result in greater total seed yield (i.e., both combined) and ec...

  19. Reduction of antinutritional glucosinolates in Brassica oilseeds by mutation of genes encoding transporters

    DEFF Research Database (Denmark)

    Nour-Eldin, Hussam Hassan; Madsen, Svend Roesen; Engelen, Steven

    2017-01-01

    The nutritional value of Brassica seed meals is reduced by the presence of glucosinolates, which are toxic compounds involved in plant defense. Mutation of the genes encoding two glucosinolate transporters (GTRs) eliminated glucosinolates from Arabidopsis thaliana seeds, but translation of loss-o...... glucosinolate content in other oilseed crops, such as Camelina sativa or Crambe abyssinica....

  20. The effect of biodiesel policies on world biodiesel and oilseed prices

    NARCIS (Netherlands)

    Drabik, D.; Gorter, de H.; Timilsina, G.R.

    2014-01-01

    A theoretical and empirical model is developed to analyze the effect of a biodiesel mandate, a tax exemption (tax credit) and an exogenous diesel price shock on world soybean and canola markets. The jointness in crushing oil and meal from the oilseed reduces the size of the link between biodiesel

  1. Gas assisted mechanical expression of cocoa butter from cocoa nibs and edible oils from oilseeds

    NARCIS (Netherlands)

    Venter, M.J.; Willems, P.; Kuipers, N.J.M.; de Haan, A.B.

    2006-01-01

    The current methods used to recover high quality oil from oilseeds have low yields (mechanical expression, aqueous extraction), require the use of toxic chemicals and rigorous purification processes that can reduce the quality of the oil (solvent extraction with hexane) or are unsuitable for the

  2. Negative priming of native soil organic carbon mineralization by oilseed biochars of contrasting quality

    NARCIS (Netherlands)

    Francischinelli Rittl, T.; Novotny, E.H.; Baliero, F.C.; Hoffland, E.; Alves, B.J.R.; Kuijper, T.W.M.

    2015-01-01

    Oilseed-derived biochar, a by-product of pyrolysis for biodiesel production, is richer in aliphatic compounds than the commonly studied wood-derived biochar, affecting both its mineralization in soil and its interaction with native soil organic carbon (nSOC). Here, we investigated the soil C

  3. Sustainability assessment of oilseed fractionation processes: A case study on lupin seeds

    NARCIS (Netherlands)

    Berghout, J.A.M.; Pelgrom, P.J.M.; Schutyser, M.A.I.; Boom, R.M.; Goot, van der A.J.

    2015-01-01

    Traditional ingredient production focusses on high purity and yield, resulting in energy- and resource-intensive fractionation processes. We explored alternative fractionation routes for oilseeds by focussing on functionality and optimal resource use. Lupin seeds were taken as model material because

  4. Investigation into the prospects of five novel oilseed crops within Europe

    NARCIS (Netherlands)

    Marvin, H.J.P.; Mastebroek, H.D.; Becu, D.M.S.; Janssens, R.J.J.

    2000-01-01

    The economic potential in Europe of five novel oilseed crops for applications within the chemical industry, particularly in paints and lubricants, was investigated within a multidisciplinary integrated research and development project. The entire production chain was subjected to a study. The

  5. Prediction of oil expression by uniaxial compression using time-varying oilseed properties

    DEFF Research Database (Denmark)

    Bargale, P. C.; Wulfsohn, Dvoralai; Irudayaraj, J.

    2000-01-01

    A mathematical simulation of uniaxial compression of oilseeds for oil extraction was developed based upon combining Terzaghi's theory of consolidation for saturated soils with Darcy's law for unsaturated flow, while incorporating the time-varying nature of the coefficients of permeability...

  6. Registration of an oilseed sunflower germplasm HA-DM1 resistant to sunflower downy mildew

    Science.gov (United States)

    HA-DM1 (Reg. No.xxx, PI 674793) sunflower (Helianthus annuus L.) germplasm was developed and released cooperatively by the USDA-ARS, Sunflower and Plant Biology Research Unit and the North Dakota Agricultural Experiment Station in 2015. HA-DM1 is a BC2F4 derived oilseed maintainer line from the cros...

  7. Violence against women in war: rape, AIDS, sex slavery. International.

    Science.gov (United States)

    At an international conference attended by 2000 delegates, violence against women in Rwanda, former Yugoslavia, and Kurdistan was discussed. Kalliope Migirou, of the United Nations Human Rights Field Operation in Rwanda, described the slaughter of between 500,000 and 1.5 million Tutsis and moderate Hutus in 1994; estimates of the number of rapes ranged from 15,700 (Rwandan government) to 250,000-500,000 (UN special representative). Women were gang-raped and sexually mutilated; fathers were forced to rape their daughters, and sons, their mothers. The transmission of HIV was used as a weapon to murder women and their communities. Women were taken to refugee camps as sex slaves and have written their families about their "new marriages" to Hutu militia men. No rape charge is found among the more than 4000 cases prepared for the Rwandan war crimes trial. 80,000 Rwandans are in prison on suspicion of participating in the genocide; 8% are women. Violete Krasnic, of the Autonomous Women's Center Against Sexual Violence in Belgrade, spoke about the war in former Yugoslavia, which increased all forms of violence against women: 1) domestic violence, particularly in inter-ethnic marriages; 2) death threats against women (up 30-50%); 3) rape (up 30%); and 4) threats with weapons (40%). Men, upon exposure to nationalistic propaganda, used violence against their wives. Nazaneen Rasheed, a London-based representative of the Women's Union of Kurdistan, stated that women in northern Iraq had no power or land. While some turned to prostitution to survive, hundreds were killed by male relatives because of shame to the family.

  8. Effect of sulfur deficiency on the resistance of oilseed rape to fungal pathogens and expression profiling of the glutathione S-transferase family of "Arabidopsis thaliana"

    OpenAIRE

    Dubuis, Pierre-Henri; Mauch, Felix

    2007-01-01

    La réduction de la pollution atmosphérique due au dioxyde de soufre a provoqué au début des années nonante des problèmes de carence de soufre dans les champs principalement au nord de l’Europe. Le soufre est un macroélément essentiel pour le développement de la plante. Des composés soufrés tels que des protéines riches en soufre, des phytoalexines et des glucosinolates, jouent un rôle important dans les mécanismes de défense. Des plants de colza carencés en soufre, mais ne montrant aucun symp...

  9. Computational analysis of storage synthesis in developing Brassica napus L. (oilseed rape) embryos: Flux variability analysis in relation to 13C-metabolic flux analysis

    Energy Technology Data Exchange (ETDEWEB)

    Hay, J.; Schwender, J.

    2011-08-01

    Plant oils are an important renewable resource, and seed oil content is a key agronomical trait that is in part controlled by the metabolic processes within developing seeds. A large-scale model of cellular metabolism in developing embryos of Brassica napus (bna572) was used to predict biomass formation and to analyze metabolic steady states by flux variability analysis under different physiological conditions. Predicted flux patterns are highly correlated with results from prior 13C metabolic flux analysis of B. napus developing embryos. Minor differences from the experimental results arose because bna572 always selected only one sugar and one nitrogen source from the available alternatives, and failed to predict the use of the oxidative pentose phosphate pathway. Flux variability, indicative of alternative optimal solutions, revealed alternative pathways that can provide pyruvate and NADPH to plastidic fatty acid synthesis. The nutritional values of different medium substrates were compared based on the overall carbon conversion efficiency (CCE) for the biosynthesis of biomass. Although bna572 has a functional nitrogen assimilation pathway via glutamate synthase, the simulations predict an unexpected role of glycine decarboxylase operating in the direction of NH4+ assimilation. Analysis of the light-dependent improvement of carbon economy predicted two metabolic phases. At very low light levels small reductions in CO2 efflux can be attributed to enzymes of the tricarboxylic acid cycle (oxoglutarate dehydrogenase, isocitrate dehydrogenase) and glycine decarboxylase. At higher light levels relevant to the 13C flux studies, ribulose-1,5-bisphosphate carboxylase activity is predicted to account fully for the light-dependent changes in carbon balance.

  10. The effect of endo- and exogenous factors on growth and maturation of oilseed rape (Brassica napus cv. Jet Neuf siliques cultured in vitro

    Directory of Open Access Journals (Sweden)

    Lucyna Drozdowska

    2014-01-01

    Full Text Available The effect of developmental stage of siliques, growth regulators, agar and sucrose concentrations in the medium was investigated on the morphogenesis of the silliques and seed maturation. Regeneration of shoots, roots and callus occurred at the base of siliques during their growth. The developmental stage of the incubated siliques was the most important factor affecting the number of seeds, while the conditions of culture (agar and growth regulators constituted a secondary factor. The reasons for the insignificant number of seeds produced could be the lack of fertilization of some of the ovules, limited ability of siliques to take up nutrients and the unspecific composition of the medium.

  11. Dynamic metabolic changes in seeds and seedlings of Brassica napus (oilseed rape) suppressing UGT84A9 reveal plasticity and molecular regulation of the phenylpropanoid pathway.

    Science.gov (United States)

    Hettwer, Karina; Böttcher, Christoph; Frolov, Andrej; Mittasch, Juliane; Albert, Andreas; von Roepenack-Lahaye, Edda; Strack, Dieter; Milkowski, Carsten

    2016-04-01

    In Brassica napus, suppression of the key biosynthetic enzyme UDP-glucose:sinapic acid glucosyltransferase (UGT84A9) inhibits the biosynthesis of sinapine (sinapoylcholine), the major phenolic component of seeds. Based on the accumulation kinetics of a total of 158 compounds (110 secondary and 48 primary metabolites), we investigated how suppression of the major sink pathway of sinapic acid impacts the metabolome of developing seeds and seedlings. In UGT84A9-suppressing (UGT84A9i) lines massive alterations became evident in late stages of seed development affecting the accumulation levels of 58 secondary and 7 primary metabolites. UGT84A9i seeds were characterized by decreased amounts of various hydroxycinnamic acid (HCA) esters, and increased formation of sinapic and syringic acid glycosides. This indicates glycosylation and β-oxidation as metabolic detoxification strategies to bypass intracellular accumulation of sinapic acid. In addition, a net loss of sinapic acid upon UGT84A9 suppression may point to a feedback regulation of HCA biosynthesis. Surprisingly, suppression of UGT84A9 under control of the seed-specific NAPINC promoter was maintained in cotyledons during the first two weeks of seedling development and associated with a reduced and delayed transformation of sinapine into sinapoylmalate. The lack of sinapoylmalate did not interfere with plant fitness under UV-B stress. Increased UV-B radiation triggered the accumulation of quercetin conjugates whereas the sinapoylmalate level was not affected. Copyright © 2016 Elsevier Ltd. All rights reserved.

  12. Non-destructive in vitro selection of microspore-derived embryos with the fertility restorer gene for CMS Ogu-INRA in winter oilseed rape (Brassica napus L.

    Directory of Open Access Journals (Sweden)

    Lenka Havlíčková

    2015-01-01

    Conclusion: Technique developed in this study provides early and non-destructive sampling of embryonic tissue and the use of new markers for simple and efficient control of the presence of Rfo gene in all accessions.

  13. Misogyny, acculturation, and ethnic identity: relation to rape-supportive attitudes in Asian American college men.

    Science.gov (United States)

    Koo, Kelly H; Stephens, Kari A; Lindgren, Kristen P; George, William H

    2012-08-01

    Asian Americans have been understudied with respect to sexuality and rape and its contributory factors. Some attitudinal research has shown that Asian American college males tend to hold more rape-supportive beliefs than their White counterparts. Generally, this research treats ethnicity as a proxy for culture rather than examining specific facets of culture per se. The current study incorporated measures of misogynistic beliefs, acculturation, and ethnic identity to investigate these ethnic differences in rape-supportive attitudes. White (n = 222) and Asian American (n = 155) college men read an acquaintance rape vignette and evaluated it on four judgments: how much they blamed the perpetrator and the victim, how credible they viewed the victim's refusal, and to what degree they defined the event as rape. Consistent with previous research, Asian American men made more rape-supportive judgments than Whites. This relationship was partially mediated by misogynistic beliefs for all judgments except the extent to which they defined the vignette as rape. Among Asian Americans, acculturation was negatively associated with all four rape vignette judgments above and beyond generational status, and ethnic identity was positively associated with two of the four judgments above and beyond acculturation and generational status. These findings suggest that cultural constructs are relevant to understanding rape-supportive attitudes among Asian American men, and may be useful for promoting culturally enhanced theoretical models of rape and sexual assault prevention efforts, as well as a deeper understanding of cultural influences on sexuality.

  14. Rape myth acceptance in men who completed the prostitution offender program of British Columbia.

    Science.gov (United States)

    Klein, Carolin; Kennedy, M Alexis; Gorzalka, Boris B

    2009-06-01

    In an effort to characterize the attitudes and characteristics of men who solicit sex, this study investigated rape myth acceptance as assessed by a modification of Burt's Rape Myth Acceptance Scale. The participants were all men who took part in the Prostitution Offender Program of British Columbia after being arrested for attempting to solicit sex from an undercover police officer. Relationships between endorsement of rape myths, other attitudes, sexual behavior, and demographic variables were examined. Results reveal that age, education, use of pornography, ideal frequency of intercourse, and believing that purchasing sex is a problem are all negatively correlated with rape myth acceptance. Positive correlations were found between rape myth acceptance and sexual conservatism, sexual violence/coercion, and social desirability. Results are discussed in terms of the association between rape myth acceptance and the violence frequently perpetrated against those working in the sex trade.

  15. Rape Crimes Reviewed: The Role of Observer Variables in Female Victim Blaming

    Directory of Open Access Journals (Sweden)

    Maria Clara Ferrão

    2015-04-01

    Full Text Available This article presents an overview of empirical research on the role of observer variables in rape victim blaming (female attacked by a male perpetrator. The focus is on literature from the last 15 years. The variables observer gender, ambivalent sexism, rape myth acceptance, and rape empathy are discussed in relation to victim blaming. Most research on rape is conducted using diverse methods and approaches that result in a great disparity regarding the role of these variables in predicting blame assignments. Despite the inconsistencies, most studies show that men hold the victim more responsible for her own victimization than women. Findings further indicate that higher scores on sexist ideologies and rape myth acceptance predict higher victim blame, and that higher rape empathy scores predict lower victim blame. Theoretical and practical implications are discussed.

  16. Wet oxidation pretreatment of rape straw for ethanol production

    DEFF Research Database (Denmark)

    Arvaniti, Efthalia; Bjerre, Anne Belinda; Schmidt, Jens Ejbye

    2012-01-01

    Rape straw can be used for production of second generation bioethanol. In this paper we optimized the pretreatment of rape straw for this purpose using Wet oxidation (WO). The effect of reaction temperature, reaction time, and oxygen gas pressure was investigated for maximum ethanol yield via...... slurry (Filter cake + filtrate) in SSF were also tested. Except ethanol yields, pretreatment methods were evaluated based on achieved glucose yields, amount of water used, recovery of cellulose, hemicellulose, and lignin.The highest ethanol yield obtained was 67% after fermenting the whole slurry...... gas produced higher ethanol yields and cellulose, hemicelluloses, and lignin recoveries, than 15 min WO treatment at 195 °C. Also, recycling filtrate and use of higher oxygen gas pressure reduced recovery of materials. The use of filtrate could be inhibitory for the yeast, but also reduced lactic acid...

  17. Restoring rape survivors: justice, advocacy, and a call to action.

    Science.gov (United States)

    Koss, Mary P

    2006-11-01

    Rape results in mental and physical health, social, and legal consequences. For the latter, restorative justice-based programs might augment community response, but they generate controversy among advocates and policy makers. This article identifies survivors' needs and existing community responses to them. Survivors feel their legal needs are most poorly met due to justice system problems that can be summarized as attrition, retraumatization, and disparate treatment across gender, class, and ethnic lines. Empirical data support each problem and the conclusion that present justice options are inadequate. The article concludes by identifying common ground in advocacy and restorative justice goals and calls for a holistic approach to the needs of rape survivors that includes advocating for expanded justice alternatives. A call to action is issued to implement restorative alternatives to expand survivor choice and offender accountability. Conventional and restorative justice are often viewed as mutually exclusive whereas the author argues they are complementary.

  18. A History of Rape in American Society Prior to 1900.

    Science.gov (United States)

    Crossman, Leslie

    By tracing the concept of rape as it has been defined by man in the earliest laws, it is evident that the criminal act was viewed with horror, and the deadly punishments that were seen fit to apply had little to do with an actual act of sexual violence that a woman might sustain. The past records indicate a gradual decrease in the prosecution rate…

  19. Worth their while?: Pursuing a rape complaint through the criminal ...

    African Journals Online (AJOL)

    If the criminal justice system were to advertise itself to victims of sexual violence, would its tag line be L'Oreal's 'Because you're worth it'? Or would the Foundry ... Certainly, a lack of faith in, or fear of, legal processes, is one of the reasons why eight out of nine women who are raped do not report the matter to the police.1 ...

  20. Aggregate-level lead exposure, gun violence, homicide, and rape.

    Science.gov (United States)

    Boutwell, Brian B; Nelson, Erik J; Qian, Zhengmin; Vaughn, Michael G; Wright, John P; Beaver, Kevin M; Barnes, J C; Petkovsek, Melissa; Lewis, Roger; Schootman, Mario; Rosenfeld, Richard

    2017-01-01

    An increasing body of research has linked the geographic distribution of lead with various indicators of criminal and antisocial behavior. The current study, using data from an ongoing project related to lead exposure in St. Louis City, MO, analyzed the association between aggregate blood lead levels and specific indicators violent crime within the city. Ecological study. St. Louis, Missouri. Blood lead levels. Official reports of violent crimes were categorized as 1) crimes involving a firearm (yes/no), 2) assault crimes (with or without a firearm), 3) robbery crimes (with or without a firearm), 4) homicides and 5) rape. With the exception of rape, aggregate blood-lead levels were statistically significant predictors of violent crime at the census tract level. The risk ratios for each of the outcome measures were as follows: firearm crimes 1.03 (1.03-1.04), assault crimes 1.03 (1.02-1.03), robbery crimes 1.03 (1.02-1.04), homicide 1.03 (1.01, 1.04), and rape 1.01 (0.99-1.03). Extending prior research in St. Louis, results suggest that aggregated lead exposure at the census tract level predicted crime outcomes, even after accounting for important sociological variables. Moving forward, a more developed understanding of aggregate level crime may necessitate a shift toward studying the synergy between sociological and biological risk factors such as lead exposure.

  1. DNA typing from vaginal smear slides in suspected rape cases

    Directory of Open Access Journals (Sweden)

    Dayse Aparecida da Silva

    Full Text Available In an investigation of suspected rape, proof of sexual assault with penetration is required. In view of this, detailed descriptions of the genitalia, the thighs and pubic region are made within the forensic medical service. In addition, vaginal swabs are taken from the rape victim and some of the biological material collected is then transferred to glass slides. In this report, we describe two rape cases solved using DNA typing from cells recovered from vaginal smear slides. In 1999, two young women informed the Rio de Janeiro Police Department that they had been victims of sexual assaults. A suspect was arrested and the victims identified him as the offender. The suspect maintained that he was innocent. In order to elucidate these crimes, vaginal smear slides were sent to the DNA Diagnostic Laboratory for DNA analysis three months after the crimes, as unique forensic evidence. To get enough epithelial and sperm cells to perform DNA analysis, we used protocols modified from the previously standard protocols used for DNA extraction from biological material fixed on glass slides. The quantity of cells was sufficient to perform human DNA typing using nine short tandem repeat (STR loci. It was 3.3 billion times more probable that it was the examined suspect who had left sperm cells in the victims, rather than any other individual in the population of Rio de Janeiro.

  2. Don't tell: Military culture and male rape.

    Science.gov (United States)

    O'Brien, Carol; Keith, Jessica; Shoemaker, Lisa

    2015-11-01

    The issue of sexual assault that occurs during military service has been a focus of attention over the past several years. Although approximately 50% of survivors of military sexual assault are men, virtually all of the literature focuses on the assault of female service members. Research has demonstrated that cultural variables are robust correlates of the sexual assault of women. This paper proposes that cultural variables are equally important when examining the rape of men, especially when this assault occurs in military contexts. We discuss male rape myths and related constructs as they are expressed within military culture. The results of data analysis from a treatment sample of veterans with military sexual trauma (MST)-related posttraumatic stress disorder (PTSD) and clinical case examples are presented to further explore the concepts. We conclude that male rape myths and related beliefs that arise from cultural norms and are further amplified and modified by military culture impact male MST survivors and delay or obstruct their recovery. Suggestions for clinical application and future research are offered to encourage further efforts in this important area of practice. (c) 2015 APA, all rights reserved).

  3. Rape in the metropolis: the geography of crime in Delhi.

    Directory of Open Access Journals (Sweden)

    Amitabh Vikram Dwivedi

    2014-12-01

    Full Text Available This paper is a study of rape in the national capital of India, and the focus is on the amendments of rape law aftermath 12 December 2012 crime. It questions at the safety and security issues of women in the metropolis of India, and it also discusses critically how laws amendments have been made aftermath this barbaric crime. Though this paper doesn’t set an inquiry yet it provides common criticism against the new amendments and it remains observational in nature, and sets a study. The temporal aspect revolves around the gang rape case of December 2012, but it also peeks in the past for the reference purpose only. The insensitive reactions from the political party leaders and religious leaders also find a space in the paper to show the general reaction of the society. The urban middle-class carefree attitude is reflected by an example of a popular reality show. But finally this paper advocates for lengthy prison sentences that have some behavior-altering deterrent value.

  4. Use of reverse micelles for the simultaneous extraction of oil, proteins, and glucosinolates from cruciferous oilseeds.

    Science.gov (United States)

    Ugolini, Luisa; De Nicola, Gina; Palmieri, Sandro

    2008-03-12

    Cruciferous oilseeds are important sources of oil, proteins, and glucosinolates (GLs), potentially available when biorefinery processes are used. The proposed extraction technology is based on the use of reverse micelles (RMs) made with cetyltrimethylammonium bromide (CTAB) dispersed in organic solvent. The physicochemical properties of this extraction system and the good water solubility of many high value compounds, such as GLs and some proteins, permit the simultaneous extraction of oil, and these products from cruciferous oilseed meals. This procedure is based on three main steps: (i) seed conditioning; (ii) solid-liquid extraction by RM solution; and (iii) back-transfer of the RM solution for recovery of the extracted compounds. The method makes it possible to simultaneously extract almost the same amount of oil as with pure organic solvents used in the current extraction plants and more than 90% of soluble proteins and GLs. It is a promising biorefinery technology alternative to traditional oil extraction processes.

  5. Sensory evaluation of commercial fat spreads based on oilseeds and walnut

    OpenAIRE

    Dimić Etelka B.; Vujasinović Vesna B.; Radočaj Olga F.; Borić Bojan D.

    2013-01-01

    The main focus of this study was on the sensory evaluation of commercial oilseeds spreads, as the most significant characteristic of this type of product from the consumers’ point of view. Sensory analysis was conducted by five experts using a quantitative descriptive and sensory profile test, applying a scoring method according to the standard procedure. Five different spreads were evaluated: sunflower, pumpkin, sesame, peanut, and walnut. Oil content and ...

  6. A continuum of research projects to improve extraction of oil and proteins in oilseed plants

    Directory of Open Access Journals (Sweden)

    Miquel Martine

    2011-05-01

    Full Text Available A key challenge in the actual context of fossil sources rarefaction, global warming, and of increase of the world global population, is to promote the use of molecules derived from renewable sources such as plants. Among these molecules, lipids and proteins are targets of interest. Plant lipids from oilseeds are attractive substitutes to the use of fossil oil. Till the beginning of the 20th century, numerous products used in the daily life were derived from natural renewable products. For instance, plant oil was commonly used as fuel for vehicles and was entering in the composition of paintings, lubricants etc. Unfortunately, natural oils have been progressively replaced by cheaper fossil oil in the fabrication of these products. Nowadays, fossil oils are becoming increasingly expensive being a finite comodity. It is thus important to reduce our dependence from fossil oil and develop substitution industries. Oilseeds contain important amounts of proteins which are mainly used in feed. As several kilograms of plant protein are needed to obtain one kilogram of animal protein, the interest toward using plant protein in food is reinforced. The developments of the use of plant lipids, as well as proteins are a major stakes for the competitiveness of European agriculture and industry, as well as for sustainable development. Extraction of oil and proteins from rapeseed has a significant cost, in term of energy and solvent uses, and finally affects the ultimate quality of the products (protein digestibility. In order to quantitatively extract seed reserves under mild conditions, it will be necessary to limit the amount of energy needed, and avoid any use of solvents. Ideally, seeds should be processed in a bio refinery. In this paper, we will describe how oilseeds store their reserves, and roadblocks for improving actual oilseed extraction processes. A continuum of research projects aimed at answering targeted questions will be presented, with selected

  7. Oilseeds native to the Cerrado have fatty acid profile beneficial for cardiovascular health

    Directory of Open Access Journals (Sweden)

    Aline Medeiros ALVES

    Full Text Available ABSTRACT Objective: To assess and compare the fatty acid composition of edible seeds and a nut native to the Cerrado (Brazilian savannah to that of traditional oilseeds. Methods: Baru almonds, Cerrado cashew nuts, and pequi almonds were extracted from the fruits using appropriate equipment. All edible seeds and nuts were roasted, except for the Brazil nut. The sample lipids were extracted via cold pressing. The fatty acids were esterified, and the fatty acid esters were analyzed by gas chromatography. Results: The native and traditional edible seeds and nuts contain mostly monounsaturated fatty acids (42.72 g to 63.44 g/100 g, except for the Brazil nut, which showed predominance of polyunsaturated fatty acids (45.48 g/100 g. Pequi almond had the highest saturated fatty acid content (36.14 g/100 g. The fatty acids with the highest concentration were oleic and linoleic acids, and palmitic acid was also found in considerable concentration in the oilseeds studied. The Cerrado cashew nut and the traditional cashew nut have similar fatty acid profiles. As for the ratio of ω-6 to ω-3, the baru almond showed the highest ratio, 9:1, which was the closest to the recommended intake of these fatty acids. Conclusion: The fatty acid profile of the edible seeds and nuts native to the cerrado is similar to those of traditional oilseeds. We suggest the inclusion of native oilseeds in the diet aiming at reducing the risk of cardiovascular disease, especially the baru almond and the cerrado cashew nut, due to the fact they have high ratio of monounsaturated fatty acids to saturated fatty acids.

  8. Dedicated Industrial Oilseed Crops as Metabolic Engineering Platforms for Sustainable Industrial Feedstock Production

    Science.gov (United States)

    Zhu, Li-Hua; Krens, Frans; Smith, Mark A.; Li, Xueyuan; Qi, Weicong; van Loo, Eibertus N.; Iven, Tim; Feussner, Ivo; Nazarenus, Tara J.; Huai, Dongxin; Taylor, David C.; Zhou, Xue-Rong; Green, Allan G.; Shockey, Jay; Klasson, K. Thomas; Mullen, Robert T.; Huang, Bangquan; Dyer, John M.; Cahoon, Edgar B.

    2016-01-01

    Feedstocks for industrial applications ranging from polymers to lubricants are largely derived from petroleum, a non-renewable resource. Vegetable oils with fatty acid structures and storage forms tailored for specific industrial uses offer renewable and potentially sustainable sources of petrochemical-type functionalities. A wide array of industrial vegetable oils can be generated through biotechnology, but will likely require non-commodity oilseed platforms dedicated to specialty oil production for commercial acceptance. Here we show the feasibility of three Brassicaceae oilseeds crambe, camelina, and carinata, none of which are widely cultivated for food use, as hosts for complex metabolic engineering of wax esters for lubricant applications. Lines producing wax esters >20% of total seed oil were generated for each crop and further improved for high temperature oxidative stability by down-regulation of fatty acid polyunsaturation. Field cultivation of optimized wax ester-producing crambe demonstrated commercial utility of these engineered crops and a path for sustainable production of other industrial oils in dedicated specialty oilseeds. PMID:26916792

  9. Dedicated Industrial Oilseed Crops as Metabolic Engineering Platforms for Sustainable Industrial Feedstock Production.

    Science.gov (United States)

    Zhu, Li-Hua; Krens, Frans; Smith, Mark A; Li, Xueyuan; Qi, Weicong; van Loo, Eibertus N; Iven, Tim; Feussner, Ivo; Nazarenus, Tara J; Huai, Dongxin; Taylor, David C; Zhou, Xue-Rong; Green, Allan G; Shockey, Jay; Klasson, K Thomas; Mullen, Robert T; Huang, Bangquan; Dyer, John M; Cahoon, Edgar B

    2016-02-26

    Feedstocks for industrial applications ranging from polymers to lubricants are largely derived from petroleum, a non-renewable resource. Vegetable oils with fatty acid structures and storage forms tailored for specific industrial uses offer renewable and potentially sustainable sources of petrochemical-type functionalities. A wide array of industrial vegetable oils can be generated through biotechnology, but will likely require non-commodity oilseed platforms dedicated to specialty oil production for commercial acceptance. Here we show the feasibility of three Brassicaceae oilseeds crambe, camelina, and carinata, none of which are widely cultivated for food use, as hosts for complex metabolic engineering of wax esters for lubricant applications. Lines producing wax esters >20% of total seed oil were generated for each crop and further improved for high temperature oxidative stability by down-regulation of fatty acid polyunsaturation. Field cultivation of optimized wax ester-producing crambe demonstrated commercial utility of these engineered crops and a path for sustainable production of other industrial oils in dedicated specialty oilseeds.

  10. Lived experiences of male intimate partners of female rape victims in Cape Town, South Africa

    Directory of Open Access Journals (Sweden)

    Evalina van Wijk

    2014-02-01

    Full Text Available Background: Sexual violence in South Africa is a major public health and social problem. Sexual assault or rape is a traumatic event which disrupts not only the life of the female rape victim, but also that of her male intimate partner (MIP, irrespective of whether he witnessed or was informed of the incident.Objectives: The study aimed to explore the lived experiences of MIPs of female rape victims and the meaning of these experiences in the six months following the partner’s rape.Method: We conducted a longitudinal hermeneutic phenomenological study. Nine purposively sampled adult MIPs were interviewed over a period of six months. The participants were in an intimate relationship with a female rape victim prior to and immediately after the rape; their partners had been treated at a specialised centre for victims of rape and sexual assault. Four interviews were conducted with each of the nine intimate partners of female rape victims: (1 within 14 days of, (2 a month after, (3 three months after, and (4 six months after the rape.Results: Two major themes emerged: being-in-the-world as a secondary victim of rape, and living in multiple worlds, those of their female partners, family, friends, society, employers or colleagues, professionals and the justice system. The participant’s familiar world became strange and even threatening, and his relationship with his partner became uncertain.Conclusion: Early supportive intervention for intimate partners of female rape victims is required to prevent on-going emotional trauma and alleviate the effects of chronic post-traumatic stress disorder and suffering at intra- and interpersonal levels.

  11. Wartime Women Rape: A Means of Moral Attack and Emasculation in Lynn Nottage’s Ruined

    Directory of Open Access Journals (Sweden)

    Elaff Ganim Salih

    2016-05-01

    Full Text Available Women rape at warfare was considered a consequence of war in the social, literary and political world for a long period of time. Some criminals of rape escaped justice and others were persecuted on the basis that they were involved in mass rape because it was a natural consequence of war. But, women are targeted with rape in time of war because they are the symbolic representation of a culture, ethnicity, and the unifying fabric of their people and nation. The objective of this paper is to show that war rape is not a result of war; instead it is a means of human destruction through moral attack and emasculation. It aims to show that women rape in warfare is neither a misogynist act nor a sexual violence but it is a pre-planned weapon used strategically and systematically to fulfill certain political and military agenda. The study focuses on the sexual abuse of women in the Democratic Republic of Congo in time of war in Lynn Nottage’s Pulitzer Prize play, Ruined (2007. The study applies Jonathan Gottschall’s Strategic Rape theory, which highlights war rape as a pre-planned military strategy. The enemy emasculates men and attacks them morally by raping their women. Consequently, men’s failure to protect their women causes them to give up resistance, leave their lands and families because of shame and humiliation. The study concludes that women rape in time of war is a tactic followed by conquerors intentionally to facilitate and guarantee the achievement of certain pre-planned goals as was the case of mass rape in the DRC.

  12. Homogeneity in Community-Based Rape Prevention Programs: Empirical Evidence of Institutional Isomorphism

    Science.gov (United States)

    Townsend, Stephanie M.; Campbell, Rebecca

    2007-01-01

    This study examined the practices of 24 community-based rape prevention programs. Although these programs were geographically dispersed throughout one state, they were remarkably similar in their approach to rape prevention programming. DiMaggio and Powell's (1991) theory of institutional isomorphism was used to explain the underlying causes of…

  13. The victimology of rape in Nigeria: Examining victims' post-assault ...

    African Journals Online (AJOL)

    The secondary victimisation suffered by rape victims in socially conservative Nigeria is not only in the hands of their families, friends, and significant others, but also through the agents and process of criminal justice system of the country. Previous research into rape in Nigeria has often neglected the aftermath of forcible ...

  14. Images of rape in African fiction. Between the assumed fatality of ...

    African Journals Online (AJOL)

    A horrendous aspect of patriarchy and dominator ideology, rape has in recent times captured the attention of civil society and the imagination of creative writers. The frequency of rape in peacetime, traditional, colonial, liberation war, and civil war contexts, since the dawn of time, seems to suggest that this gendered violence ...

  15. Attitudes Toward Victims of Rape: Effects of Gender, Race, Religion, and Social Class

    Science.gov (United States)

    Nagel, Barbara; Matsuo, Hisako; McIntyre, Kevin P.; Morrison, Nancy

    2005-01-01

    Although previous literature focusing on perceptions of victims of rape has examined how gender, race, and culture influence the attitudes one holds toward victims, these studies have yielded mixed results. This study compared perceptions of victims of rape across a wide range of ages, educational backgrounds, religions, and income levels, while…

  16. Rape Culture and Campus Environment: An Introduction for Student Affairs Professionals.

    Science.gov (United States)

    Watson, Lemeul W.; Derby, Dustin

    2000-01-01

    Provides a brief introduction for student affairs professional to the American rape culture. Offers suggestions and examples to assist student affairs professionals in their quest to develop adequate programs and services on their campus with regard to rape and sexual assault incidents. (Contains 31 references.) (GCP)

  17. Ethical problems of rape as a social cankerworm in our present day ...

    African Journals Online (AJOL)

    Rape is a worrisome epidemic that is becoming problematic in contemporary Nigeria due to many factors that are already inherent in the Nigerian society such as myths, culture of silence associated with rape, modernizing influences and peer group pressure among several others. This article is essentially library ...

  18. Predicting Perceptions of Date Rape: An Examination of Perpetrator Motivation, Relationship Length, and Gender Role Beliefs

    Science.gov (United States)

    Angelone, David J.; Mitchell, Damon; Lucente, Lauren

    2012-01-01

    The purpose of the current study is to examine the influence of multiple offender motivations (including no indication of a motivation), relationship length, and gender role beliefs on perceptions of a male-on-female date rape. A sample of 348 U.S. college students read a brief vignette depicting a date rape and completed a questionnaire regarding…

  19. 76 FR 20711 - Hearings of the Review Panel on Prison Rape

    Science.gov (United States)

    2011-04-13

    ... Office of Justice Programs Hearings of the Review Panel on Prison Rape AGENCY: Office of Justice Programs... Review Panel on Prison Rape (Panel) will hold hearings in Washington, DC on April 26-27, 2011. The... U.S. prisons, and the common characteristics of prisons with the highest and lowest incidence of...

  20. 76 FR 56481 - Hearings of the Review Panel on Prison Rape

    Science.gov (United States)

    2011-09-13

    ... of Justice Programs Hearings of the Review Panel on Prison Rape AGENCY: Office of Justice Programs... Review Panel on Prison Rape (Panel) will hold hearings in Washington, DC on September 15-16, 2011. The... August 26, 2010, the BJS issued the report Sexual Victimization in Prisons and Jails Reported by Inmates...