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Sample records for brine shrimp artemia

  1. The brine shrimp Artemia: adapted to critical life conditions

    Directory of Open Access Journals (Sweden)

    Gonzalo M Gajardo

    2012-06-01

    Full Text Available The brine shrimp Artemia is a micro-crustacean, well adapted to the harsh conditions that severely hypersaline environments impose on survival and reproduction. Adaptation to these conditions has taken place at different functional levels or domains, from the individual (molecular-cellular-physiological to the population level. Such conditions are experienced by very few equivalent macro-planktonic organisms; thus, Artemia can be considered a model animal extremophile offering a unique suite of adaptations that are the focus of this review. The most obvious is a highly efficient osmoregulation system to withstand up to 10 times the salt concentration of ordinary seawater. Under extremely critical environmental conditions, for example when seasonal lakes dry out, Artemia takes refuge by producing a highly resistant encysted gastrula embryo (cyst capable of severe dehydration enabling an escape from population extinction. Cysts can be viewed as gene banks that store a genetic memory of historical population conditions. Their occurrence is due to the evolved ability of females to perceive forthcoming unstable environmental conditions expressed by their ability to switch reproductive mode, producing either cysts (oviparity when environmental conditions become deleterious or free-swimming nauplii (ovoviviparity that are able to maintain the population under suitable conditions.At the population level the trend is for conspecific populations to be fragmented into locally adapted populations, whereas species are restricted to salty lakes in particular regions (regional endemism. The Artemia model depicts adaptation as a complex response to critical life conditions, integrating and refining past and present experiences at all levels of organization. Although we consider an invertebrate restricted to a unique environment, the processes to be discussed are of general biological interest. Finally, we highlight the benefits of understanding the stress

  2. Laboratory and field studies on an Indian strain of the brine shrimp Artemia

    Digital Repository Service at National Institute of Oceanography (India)

    Royan, J.P.

    The Tuticorin strain of the brine shrimp @iArtemia@@ was studied under both laboratory and field conditions. Studies on the survival of the nauplii at different temperatures and salinities revealed that the nauplii preferred a salinity of 35 ppt...

  3. Evaluation of the bioactivities of some Myanmar medicinal plants using brine shrimp (Artemia salina) toxicity test

    International Nuclear Information System (INIS)

    Sabai; Khin Khin Win Aung; Nwe Ni Thin; Kyi Shwe; Tin Myint Htwe

    2001-01-01

    For a variety of toxic substances, brine shrimp larvae (Artemia salina) are usually used as a simple bioassay method and it is also applied for natural product research. The brine shrimp larvae (nauplii) are obtained by natural hatching method from Artemia cysts. By using the larvae, the results from these experiments lead to the lethal dose, LD 50 values of extracts of selected medicinal plants. Activities of a broad range of plant extracts are manifested as toxicity to the brine shrimp. Screening results with six plant extracts are compared with pure caffeine. This method is rapid, reliable, inexpensive and convenient. (author)

  4. Brine shrimp bioassay: importance of correct taxonomic identification of Artemia (Anostraca) species.

    Science.gov (United States)

    Ruebhart, David R; Cock, Ian E; Shaw, Glen R

    2008-08-01

    Despite the common use of the brine shrimp bioassay in toxicology, there is confusion in the literature regarding citation of the correct taxonomic identity of the Artemia species used. The genus Artemia, once thought to be represented by a single species Artemia salina, is now known to be composed of several bisexual species as well as parthenogenetic populations. Artemia franciscana is the best studied of the Artemia species and is considered to represent the vast majority of studies in which Artemia is used as an experimental test organism. We found that in studies referring to the use of A. salina, the zoogeography of the cyst harvest site indicated that the species used was actually A. franciscana. Those performing bioassays with Artemia need to exercise diligence in assigning correct species identification, as the identity of the test organism is an important parameter in assuring the validity of the results of the assay.

  5. Review of the African distribution of the brine shrimp genus Artemia ...

    African Journals Online (AJOL)

    Brine shrimp (genus Artemia) are small (8 to 12 mm long) cosmopolitan crustaceans (Anostraca) found predominately in hypersaline water bodies such as inland salt lakes and pans, coastal lagoons, and salt works at salinity levels above 40 g·ℓ-1. They have been extensively studied due to their high monetary value as ...

  6. [Immunocytochemical studies on the phase of differentiation of hatching gland cells in brine shrimp, Artemia salina].

    Science.gov (United States)

    Li, Ling; Fan, Ting Jun; Wang, Xiao Feng; Cong, Ri Shan; Yu, Qiu Tao; Zhong, Qi Wang

    2004-04-01

    Hatching enzyme (HE), synthesized in hatching gland cells (HGCs), plays vital roles in animal hatching. Immunocytochemical techniques employing anti-GST-UVS.2 antiserum, prepared from Xenopus HE and with specificity to brine shrimp HE, were first used to investigate the differentiation and variability of hatching gland cells (HGCs) in the hatching process of embryos of brine shrimp, Artemia salina, in this study. HGCs with immunoreactivity to anti-GST-UVS.2 antiserum were identified, for the first time, in brine shrimp embryos during hatching process. Immunocytochemical staining results showed that, (1) HE-positive immunoreactivity is really specific to Artemia HE, and its appearance and disappearance are closely correlated with the hatching process of Artemia salina. (2) Artemia HGCs, first appeared in embryos 5 hours before hatching and disappeared 4 hours after hatching, were also a transient type of cells, with an existence period of 9 hours. (3) The head portion of Artemia embryo is probably the initial position of HE secretion, and likely to be the main position of HE secretion as well. The detailed process and mechanism need to be studied. (4) The appearance of HGCs is in a synchronous mode from places all over the embryos, and their disappearance is also in a synchronous mode. (5) The number of HGCs increased gradually along with embryo development process and reached a maximum number at hatching. Contrarily, the number of HGCs decreased gradually after hatching, and HGCs disappeared 5 hours after hatching. However, the intensity of HE-positive reaction was almost at the same level at the period of HGCs'presence. (6) Artemia HGCs were distributed throughout the body of embryos at all time during their presence. Therefore, it can concluded that Artemia HGCs, as a transient type of cells, first appeared in embryos 4 hours before hatching and disappeared in embryos 5 hours after hatching, and with distinguished patterns of appearance, disappearance and

  7. Biological screening of chitosan derivatives using Artemia spp. (brine shrimp test)

    International Nuclear Information System (INIS)

    Rozaini Mohd Zohdi; Norimah Yusof; Asnah Hassan

    2006-01-01

    The present study reported on the screening of six selected chitosan derivatives using the brine shrimp lethality bioassay. In addition, the irradiation effects towards the compounds at 25 kGy were also studied. Chitosan is a natural polysaccharide derived from chitin, extracted from the exoskeletons of crustaceans and insects as well as walls of some bacteria and fungi. Brine shrimp test is employed for the screening of toxicity of chitosan derivatives. Toxicity test was carried out by adding different concentrations of tested samples to approximately 5 to 15 Artemia salina larvae. Biological activity using the brine shrimp bioassay was recorded as LC 50 i.e. lethal concentration that kills 50% of the larvae within 24 hours of contact with the samples. Compounds are considered toxic when the LC 50 value is lower than 1 mg/ml by brine shrimp bioassay and practically non-toxic when the value is larger. Of the samples tested, none were toxic to the brine shrimp (LC 50 > 1 mg/ml). The LC 50 values of all chitosan derivatives tested, control and irradiated at 25 kGy were above 1 mg/ml thus all tested samples are considered non-toxic. This study demonstrated that irradiation at 25 kGy showed no significant effects towards the toxicity of the chitosan derivatives. After irradiation, only NO-CMC exhibited marked decrease in LC 50 value, reduced by 3-fold from 34.96 mg/ml to 11.07 mg/ml while O-CMC (5.45 mg/ml to 5 mg/ml) showed no clear differences based on rough estimation. This study suggested that brine shrimp bioassay is a simple, reliable and convenient method that could provide useful clues of the relative toxic potential of the sample tested. (Author)

  8. The application of bioflocs technology to protect brine shrimp (Artemia franciscana) from pathogenic Vibrio harveyi.

    Science.gov (United States)

    Crab, R; Lambert, A; Defoirdt, T; Bossier, P; Verstraete, W

    2010-11-01

    To study the potential biocontrol activity of bioflocs technology. Glycerol-grown bioflocs were investigated for their antimicrobial and antipathogenic properties against the opportunistic pathogen Vibrio harveyi. The bioflocs did not produce growth-inhibitory substances. However, bioflocs and biofloc supernatants decreased quorum sensing-regulated bioluminescence of V. harveyi. This suggested that the bioflocs had biocontrol activity against this pathogen because quorum sensing regulates virulence of vibrios towards different hosts. Interestingly, the addition of live bioflocs significantly increased the survival of gnotobiotic brine shrimp (Artemia franciscana) larvae challenged to V. harveyi. Bioflocs grown on glycerol as carbon source inhibit quorum sensing-regulated bioluminescence in V. harveyi and protect brine shrimp larvae from vibriosis. The results presented in this study indicate that in addition to water quality control and in situ feed production, bioflocs technology could help in controlling bacterial infections within the aquaculture pond. © 2010 The Authors. Journal of Applied Microbiology © 2010 The Society for Applied Microbiology.

  9. Cestodes change the isotopic signature of brine shrimp, Artemia, hosts: implications for aquatic food webs.

    Science.gov (United States)

    Sánchez, Marta I; Varo, Nico; Matesanz, Cristina; Ramo, Cristina; Amat, Juan A; Green, Andy J

    2013-01-01

    To reach the final host (greater flamingos), the cestode Flamingolepis liguloides alters the behaviour of its intermediate host, the brine shrimp, Artemia parthenogenetica, causing it to spend more time close to the water surface. During summer 2010, we showed that the prevalence of this cestode was consistently higher at the top of the water column in the Odiel salt pans in south-western Spain. We used stable nitrogen (N) and carbon (C) isotopic analysis to test the hypothesis that cestodes also alter resource use by Artemia. In early summer, we compared stable isotopes in infected hosts at the surface with those from uninfected hosts at the bottom of the water column. In late summer, we compared infected and uninfected Artemia from the bottom. δ(15)N was consistently enriched in infected individuals compared with uninfected hosts, especially in Artemia with multiple infections of F. liguloides (family Hymenolepididae) and those with mixed infections of F. liguloides and cestodes of the family Dilepididae. Infected individuals from the surface were enriched in δ(13)C compared with uninfected ones from the bottom, but the opposite was found when comparing uninfected and infected Artemia from the same depth. This may be caused by the increase in lipid concentration in infected Artemia. Isolated cysticercoids of F. liguloides were significantly enriched in δ(13)C compared with cysticercoids in infected hosts, but surprisingly were not enriched in N. Our findings illustrate the way cestodes can alter food webs and highlight the importance of considering the parasitic status of prey in studies of trophic ecology in saline wetlands. Copyright © 2012 Australian Society for Parasitology Inc. Published by Elsevier Ltd. All rights reserved.

  10. Toxicоlogical evaluation of the plant products using Brine Shrimp (Artemia salina L. model

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    Меntor R. Hamidi

    2014-04-01

    Full Text Available Many natural products could serve as the starting point in the development of modern medicines because of their numerous biological and pharmacological activities. However, some of them are known to carry toxicological properties as well. In order to achieve a safe treatment with plant products, numerous research studies have recently been focused on both pharmacology and toxicity of medicinal plants. Moreover, these studies employed efforts for alternative biological assays. Brine Shrimp Lethality Assay is the most convenient system for monitoring biological activities of various plant species. This method is very useful for preliminary assessment of toxicity of the plant extracts. Rapidness, simplicity and low requirements are several advantages of this assay. However, several conditions need to be completed, especially in the means of standardized experimental conditions (temperature, pH of the medium, salinity, aeration and light. The toxicity of herbal extracts using this assay has been determined in a concentration range of 10, 100 and 1000 µg/ml of the examined herbal extract. Most toxicity studies which use the Brine Shrimp Lethality Assay determine the toxicity after 24 hours of exposure to the tested sample. The median lethal concentration (LC50 of the test samples is obtained by a plot of percentage of the dead shrimps against the logarithm of the sample concentration. LC50 values are estimated using a probit regression analysis and compared with either Meyer’s or Clarkson’s toxicity criteria. Furthermore, the positive correlation between Meyer’s toxicity scale for Artemia salina and Gosselin, Smith and Hodge’s toxicity scale for higher animal models confirmed that the Brine Shrimp Lethality Assay is an excellent predictive tool for the toxic potential of plant extracts in humans.

  11. Nano-sized polystyrene affects feeding, behavior and physiology of brine shrimp Artemia franciscana larvae.

    Science.gov (United States)

    Bergami, Elisa; Bocci, Elena; Vannuccini, Maria Luisa; Monopoli, Marco; Salvati, Anna; Dawson, Kenneth A; Corsi, Ilaria

    2016-01-01

    Nano-sized polymers as polystyrene (PS) constitute one of the main challenges for marine ecosystems, since they can distribute along the whole water column affecting planktonic species and consequently disrupting the energy flow of marine ecosystems. Nowadays very little knowledge is available on the impact of nano-sized plastics on marine organisms. Therefore, the present study aims to evaluate the effects of 40nm anionic carboxylated (PS-COOH) and 50nm cationic amino (PS-NH2) polystyrene nanoparticles (PS NPs) on brine shrimp Artemia franciscana larvae. No signs of mortality were observed at 48h of exposure for both PS NPs at naplius stage but several sub-lethal effects were evident. PS-COOH (5-100μg/ml) resulted massively sequestered inside the gut lumen of larvae (48h) probably limiting food intake. Some of them were lately excreted as fecal pellets but not a full release was observed. Likewise, PS-NH2 (5-100µg/ml) accumulated in larvae (48h) but also adsorbed at the surface of sensorial antennules and appendages probably hampering larvae motility. In addition, larvae exposed to PS-NH2 undergo multiple molting events during 48h of exposure compared to controls. The activation of a defense mechanism based on a physiological process able to release toxic cationic NPs (PS-NH2) from the body can be hypothesized. The general observed accumulation of PS NPs within the gut during the 48h of exposure indicates a continuous bioavailability of nano-sized PS for planktonic species as well as a potential transfer along the trophic web. Therefore, nano-sized PS might be able to impair food uptake (feeding), behavior (motility) and physiology (multiple molting) of brine shrimp larvae with consequences not only at organism and population level but on the overall ecosystem based on the key role of zooplankton on marine food webs. Copyright © 2015 Elsevier Inc. All rights reserved.

  12. Assessment of cyst production potential of a natural population of brine shrimp Artemia

    Digital Repository Service at National Institute of Oceanography (India)

    Royan, J.P.; Sumitra-Vijayaraghavan; Krishnakumari, L.

    Studies on a natural population of Artemia in the salterns of Jamnagar indicated that the population is parthenogenetic. These shrimps reach a maximum size of 9 mm. Number of cysts per brood varies from 10-32. Adults form about 68% of the total...

  13. Temperature-dose relationships with aflatoxin M1 in milk on the brine shrimp (Artemia salina larvae

    Directory of Open Access Journals (Sweden)

    Lejla Duraković

    2011-06-01

    Full Text Available Temperature-dose relationships with aflatoxin M1 (AFM1 were studied using the brine shrimp Artemia salina larvae as an biological indicator in the temperature range from 20 °C to 40 °C. Increase in the incubation temperature resulted in sensitivity increase by the brine shrimp to AFM1. Optimum sensitivity occured at 30 °C. Positive results were obtained at 0.18 μg AFM1 x L-1 of whole pasteurized milk with a mortality of over 15%. Greater than 90 % mortality occurred at dose levels of 0.9 μg AFM1 x L-1 and above. The test can be conducted during 30-60 hours.

  14. The Brine Shrimp Artemia Survives in Diluted Water of Lake Bunyampaka, an Inland Saline Lake in Uganda

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    Martin Sserwadda

    2018-02-01

    Full Text Available Ugandan aquaculture is in the process of development; however, it requires access to an affordable live food source, such as brine shrimp Artemia. This study fits within a broader feasibility study of domestic Artemia production in salt lakes. Since Uganda is a landlocked country, the only opportunity for live water food sources lies in the salt lakes in the west of the country. This study used saline water from one of these lakes, Lake Bunyampaka (salinity 72 mg L−1. Two Artemia strains, i.e., the Great Salt Lake strain, which is the dominant strain on the market, and the Vinh Chau strain, which is by far the most inoculated strain in the world, were assayed for their survival, growth, and reproduction in diluted Lake Bunyampaka water, using natural seawater as control. The organisms were fed live freshly cultured microalgae Tetraselmis suecica ad libitum. Our study revealed that the Vinh Chau strain performed especially well in Lake Bunyampaka water diluted to 50 g L−1. The data presented in this study generate the first useful information for the future inoculation of Artemia in Lake Bunyampaka in Uganda, and hence domestic Artemia production in the country; however, further larger-scale laboratory work, followed by field trials, is still needed.

  15. Effects of gamma radiation on the survival and growth of brine shrimp, Artemia salina

    International Nuclear Information System (INIS)

    Engel, D.W.; Davis, E.M.

    1976-01-01

    To determine the effects of gamma radiation on the survival and growth of brine shrimp, nauplii and adults were exposed to different doses of radiation and maintained at different salinities and temperatures. The LD 50 determination demonstrated that nauplii were thirty to forty times more sensitive than the adults and had a 25-day LD 50 of 450 rads. Radiation and salinity reduced survival of adult female brine shrimp more than the males. The interaction of salinity, 5 and 50 ppt, temperature, 10, 20 and 30 0 C, and radiation, 25 to 100 kilorads, decreased the survival of both male and female brine shrimp, with the males having the greater sensitivity. Brine shrimp nauplii irradiated with doses of 500 and 2,500 rads had accelerated growth and matured earlier than the controls. A test of the effect of crowding on growth showed that volume per individual was important, and a dose of 500 rads accelerated brine shrimp growth in all concentrations, with the greatest increase at 8 ml/nauplius

  16. Toxicity of Diuron and copper pyrithione on the brine shrimp, Artemia franciscana: the effects of temperature and salinity.

    Science.gov (United States)

    Koutsaftis, Apostolos; Aoyama, Isao

    2008-12-01

    Diuron and copper pyrithione (CuPT) are two substances that have been used worldwide as alternatives to tributyltin (TBT) in antifouling paints for the protection of ship hulls. In this study their toxicity against the brine shrimp Artemia franciscana is examined under several combinations of salinity and temperature using the LC(20), LC(50) and LC(80) values found for the 25 degrees C and 35 per thousand standard conditions. A significant interaction between temperature and salinity effects was observed for both chemicals. Decreasing temperature almost eliminated Diuron's toxicity, while a toxicity reduction was also observed for CuPT. Decreasing salinity decreased Diuron's toxicity, while for CuPT the effect of salinity was more complex. These two natural environmental parameters had a profound influence on the ecotoxicity of the two tested chemicals, and this highlights the importance of considering the implications of such factors when conducting ecological risk assessment.

  17. Toxicity Testing of Restorative Dental Materials Using Brine Shrimp Larvae (Artemia salina

    Directory of Open Access Journals (Sweden)

    Manar M. Milhem

    2008-08-01

    Full Text Available This study investigated the effect of extracts of different composites, glass ionomer cement (GICs and compomers on the viability of brine shrimp larvae. Ethanolic extracts of four dental composites (Z-100; Solitaire 2; Filtek P60 and Synergy, a conventional GIC (Ketac-Fil, a resin-modified glass ionomer cement (Vitremer, two compomers (F2000; Dyract AP, and a flowable compomer (Dyract Flow were prepared from each material. Following evaporation of the ethanol, the extracts were resuspended in distilled water, which was then used to test the effects on the viability of brine shrimp larvae. For the composites, the extract of Synergy was the least toxic (88% viability followed by the extracts of Solitaire 2, Z100 and P60 (75%, 67.5% and 50% viability, respectively. One-way ANOVA revealed highly significant differences between the resin composite materials (p<0.001. Follow-up comparison between the composite groups by Tukey's pairwise multiple-comparison test (α =0.05 showed that the extract of Synergy was significantly less toxic than the extracts of all the other materials except that of Solitaire 2. The compomers showed 100% lethality, while the percentage of viable larvae for the extracts of Ketac-Fil, and Vitremer were 32.3%, and 37.0%, respectively. One-way ANOVA revealed highly significant differences between the groups of materials (p<0.001. Follow-up comparison between the groups by Tukey's test (α = 0.05 showed that the toxic effect of the extracts of the compomers were significantly greater than that of Ketac-Fil, and Vitremer. The differences in the toxic effects of Vitremer and Ketac-Fil were not statistically significant. In conclusion, the toxicity of composite materials varied according to their chemical composition. Compomers were the most lethal materials to brine shrimp larvae followed by GICs and then composites.

  18. Toxicity testing of restorative dental materials using brine shrimp larvae (Artemia salina).

    Science.gov (United States)

    Milhem, Manar M; Al-Hiyasat, Ahmad S; Darmani, Homa

    2008-01-01

    This study investigated the effect of extracts of different composites, glass ionomer cement (GIC)s and compomers on the viability of brine shrimp larvae. Ethanolic extracts of four dental composites (Z-100; Solitaire 2; Filtek P60 and Synergy), a conventional GIC (Ketac-Fil), a resin-modified glass ionomer cement (Vitremer), two compomers (F2000; Dyract AP), and a flowable compomer (Dyract Flow) were prepared from each material. Following evaporation of the ethanol, the extracts were resuspended in distilled water, which was then used to test the effects on the viability of brine shrimp larvae. For the composites, the extract of Synergy was the least toxic (88% viability) followed by the extracts of Solitaire 2, Z100 and P60 (75%, 67.5% and 50% viability, respectively). One-way ANOVA revealed highly significant differences between the resin composite materials (plarvae for the extracts of Ketac-Fil, and Vitremer were 32.3%, and 37.0%, respectively. One-way ANOVA revealed highly significant differences between the groups of materials (pshrimp larvae followed by GICs and then composites.

  19. Effect of temperature and viscosity on swimming velocity of the copepod Acartia tonsa, brine shrimp Artemia salina and rotifer Brachionus plicatilis

    DEFF Research Database (Denmark)

    Larsen, Poul Scheel; Madsen, C.V.; Riisgard, H.U.

    2008-01-01

    Beating cilia are important organelles for swimming in many zooplanktonic aquatic organisms, including many invertebrate larvae, rotifers and ciliates, but other planktonic organisms, such as copepods and brine shrimps, use muscle-powered swimming appendages. In recent studies we found...... of swimming velocity for a 10 degrees C temperature reduction) that is found to be largest for the brine shrimp Artemia salina nauplius (37 %) and the rotifer Brachionus plicatilis (26%), but negligible for the copepod Acartia tonsa (4%). We suggest that experimental data on change in swimming velocity (V......) due to change in kinematic viscosity (v) be correlated in terms of a power law, V proportional to v(-m). The present data on swimming velocity of copepods, brine shrimps and rotifers show values of exponent m approximate to 1.5 to 3, with a trend of decreasing values for increasing size of species...

  20. cis-Canthaxanthins: unusual carotenoids in the eggs and the reproductive system of female brine shrimp Artemia

    OpenAIRE

    Nelis, H.J.C.F.; Lavens, P.; Moens, L.; Sorgeloos, P.; Jonckheere, J.A.; Criel, G.R.J.; De Leenheer, A.P.

    1984-01-01

    The significance of carotenoid accumulation in crustacean eggs remains obscure, particularly because neither eggs nor female animals have been found to display specific pigment patterns in relation to reproduction. We report here the first example of carotenoids found exclusively in the ovaries, the eggs, and the hemolymph, but not in the carcass of a female, reproductively active crustacean, i.e. the brine shrimp Artemia . These pigments are virtually absent in males and in immature animals ...

  1. Review of the African distribution of the brine shrimp genus Artemia

    African Journals Online (AJOL)

    2006-10-04

    Oct 4, 2006 ... The life cycle of Artemia can begin as an embryo ..... belong to different salt production companies and, although geo- graphically close to each other, were not listed as being the same due to different management. Similarly, sites listed under the name Velddrif do not all belong to the same salt company. In.

  2. Evaluation of different feeds for optimal growth and survival of parthenogenetic brine shrimp, Artemia

    Digital Repository Service at National Institute of Oceanography (India)

    Sumitra-Vijayaraghavan; Krishnakumari, L.; Royan, J.P.

    intestinalis and Ulva fasciata). Rice bran yielded best growth at an optimal level of 0.1 and 0.5 mg.ml-1 d-1 respectively for 1-4 and 4-8 d old larvae. Rice bran was also the best food for outdoor cultures. Artemia fed rice bran took shortest time to reach...

  3. Differential protein expression using proteomics from a crustacean brine shrimp (Artemia sinica) under CO2-driven seawater acidification.

    Science.gov (United States)

    Chang, Xue-Jiao; Zheng, Chao-Qun; Wang, Yu-Wei; Meng, Chuang; Xie, Xiao-Lu; Liu, Hai-Peng

    2016-11-01

    Gradually increasing atmospheric CO 2 partial pressure (pCO 2 ) has caused an imbalance in carbonate chemistry and resulted in decreased seawater pH in marine ecosystems, termed seawater acidification. Anthropogenic seawater acidification is postulated to affect the physiology of many marine calcifying organisms. To understand the possible effects of seawater acidification on the proteomic responses of a marine crustacean brine shrimp (Artemia sinica) three groups of cysts were hatched and further raised in seawater at different pH levels (8.2 as control and 7.8 and 7.6 as acidification stress levels according to the predicted levels at the end of this century and next century, respectively) for 1, 7 and 14 days followed by examination of the protein expression changes via two-dimensional gel electrophoresis. Searches of protein databases revealed that 67 differential protein spots were altered due to lower pH level (7.6 and 7.8) stress in comparison to control groups (pH 8.2) by mass spectrometry. Generally, these differentially expressed proteins included the following: 1) metabolic process-related proteins involved in glycolysis and glucogenesis, nucleotide/amino acid/fatty acid metabolism, protein biosynthesis, DNA replication and apoptosis; 2) stress response-related proteins, such as peroxiredoxin, thioredoxin peroxidase, 70-kDa heat shock protein, Na/K ATPase, and ubiquinol-cytochrome c reductase; 3) immune defence-related proteins, such as prophenoloxidase and ferritin; 4) cytoskeletal-related proteins, such as myosin light chain, TCP1 subunit 2, tropomyosin and tubulin alpha chain; and 5) signal transduction-related proteins, such as phospholipase C-like protein, 14-3-3 zeta, translationally controlled tumour protein and RNA binding motif protein. Taken together, these data support the idea that CO 2 -driven seawater acidification may affect protein expression in the crustacean A. sinica and possibly also in other species that feed on brine shrimp in the

  4. The Cytotoxicity Study of Carboxymethyl Starch (CMS) of Sago Starch (Metro xylon sago) by Brine Shrimp Lethality Test (Artemia salina nauplii)

    International Nuclear Information System (INIS)

    Ibrahim Ijang; Fazliana Mohd Saaya; Zainon Othman

    2014-01-01

    CMS can be produced by substitution of the hydroxyl groups with sodium monochloroacetate in the presence of strong alkali. Carboxy methylation can be performed in water as a solvent or in a water-miscible organic solvent containing a small amount of water such as ethanol, isopropanol, methanol or toluene. The use of organic solvent will preserve the final product in the granular form and the side product can be washed out easily but some of them may be having potential toxicity and carcinogenic effect. In this study, CMS was investigated the level of toxicity by using brine shrimp lethality (BSLT). Brine shrimp test method was used to screen CMS for their biological activity. The screening results showed that the LC50, of CMS is more than 100 mg/ ml dose concentration. In conclusion, CMS is not cytotoxicity to Artemia salina nauplii and BSLT method is simple, inexpensive and convenient assay for the detection of cytotoxic compound. (author)

  5. Mercury antagonists: loss of photoactive response in the brine shrimp Artemia and its prevention by thiamine

    Energy Technology Data Exchange (ETDEWEB)

    Yaeger, E.; Siegel, B.Z.; Siegel, S.M.; Lasconia, M.; Correal, T.

    1986-01-01

    The positive photoatactic behavior of Artemia was disturbed when larvae cultured in 0.25 M NaCl were introduced into 10/sup -7/ to 10/sup -5/ M HgCl/sub 2/. At 10/sup -8/ M, and in controls, Hg had no effect either on orientation or mortality, and 65% of the larvae were collected at the light source in 30 min or less. In contrast, at 10/sup -6/ M, only 29% were able to do so. The presence of 10/sup -4/ M thiamine hydrochloride in the excystment medium prevented fully the loss of light-sensitivity.

  6. Bacillus sp. LT3 improves the survival of gnotobiotic brine shrimp (Artemia franciscana) larvae challenged with Vibrio campbellii by enhancing the innate immune response and by decreasing the activity of shrimp-associated vibrios.

    Science.gov (United States)

    Niu, Yufeng; Defoirdt, Tom; Baruah, Kartik; Van de Wiele, Tom; Dong, Shuanglin; Bossier, Peter

    2014-10-10

    Bacteria belonging to the genus Bacillus are amongst the most intensively studied group of bacteria for use as probiotics in aquaculture. However, the exact mechanism of action of these bacteria is often not well described, and the microbiota that are naturally present in cultures of test organisms often compromise the interpretation of the results. The present study aimed to evaluate the putative probiotic effect of Bacillus sp. LT3 in a model system with gnotobiotic brine shrimp Artemia franciscana larvae. The strain significantly increased the survival of brine shrimp larvae challenged with Vibrio campbellii when administered 6h before the challenge. Under these conditions, LT3 was able to colonize the brine shrimp gastrointestinal tract and to decrease the in vivo pathogen activity as indicated by the bioluminescence of the V. campbellii associated with brine shrimp larvae. In order to investigate the effect of the Bacillus strain on the innate immune system of the brine shrimp larvae, prophenoloxidase and transglutaminase mRNA levels were monitored, while heat shock protein 70 mRNA levels were measured as an indicator of physiological stress. Interestingly, 12h after challenge, the prophenoloxidase mRNA level in the larvae pre-treated with LT3 and challenged with V. campbellii was approximately 8-fold higher than in the other treatments. Further, a decreased mRNA level of transglutaminase gene and heat shock protein 70 gene suggested that pretreatment with LT3 results in less stress and tissue damage in the brine shrimp larvae upon V. campbellii challenge. These results indicated that Bacillus sp. LT3 could improve the survival of brine shrimp larvae when challenged with pathogenic V. campbellii, both by decreasing the in vivo activity of the pathogen and by priming the innate immune response through activating the prophenoloxidase system. Copyright © 2014 Elsevier B.V. All rights reserved.

  7. Comparative evaluation of impact of Zn and ZnO nanoparticles on brine shrimp (Artemia salina) larvae: effects of particle size and solubility on toxicity.

    Science.gov (United States)

    Ates, Mehmet; Daniels, James; Arslan, Zikri; Farah, Ibrahim O; Rivera, Hilsamar Félix

    2013-01-01

    Brine shrimp (Artemia salina) larvae were exposed to different sizes of zinc (Zn) and zinc oxide (ZnO) nanoparticles (NPs) to evaluate their toxicity in marine aquatic ecosystems. Acute exposure was conducted in seawater with 10, 50 and 100 mg L(-1) concentrations of the NPs for 24 h and 96 h. Phase contrast microscope images confirmed the accumulation of the NPs inside the guts. Artemia were unable to eliminate the ingested particles, which was thought to be due to the formation of massive particles in the guts. Although the suspensions of the NPs did not exhibit any significant acute toxicity within 24 h, mortalities increased remarkably in 96 h and escalated with increasing concentration of NP suspension to 42% for Zn NPs (40-60 nm) (LC50∼ 100 mg L(-1)) and to about 34% for ZnO NPs (10-30 nm) (LC50 > 100 mg L(-1)). The suspensions of Zn NPs were more toxic to Artemia than those of ZnO NPs under comparable regimes. This effect was attributed to higher Zn(2+) levels (ca. up to 8.9 mg L(-1)) released to the medium from Zn NPs in comparison to that measured in the suspensions of ZnO NPs (ca. 5.5 mg L(-1)). In addition, the size of the nanopowders appeared to contribute to the observed toxicities. Although the suspensions possessed aggregates of comparable sizes, smaller Zn NPs (40-60 nm) were relatively more toxic than larger Zn NPs (80-100 nm). Likewise, the suspensions of 10-30 nm ZnO NPs caused higher toxicity than those of 200 nm ZnO NPs. Lipid peroxidation levels were substantially higher in 96 h (p < 0.05), indicating that the toxic effects were due to the oxidative stress.

  8. A novel model of early development in the brine shrimp, Artemia franciscana, and its use in assessing the effects of environmental variables on development, emergence, and hatching.

    Science.gov (United States)

    Neumeyer, Courtney H; Gerlach, Jamie L; Ruggiero, Kristin M; Covi, Joseph A

    2015-03-01

    The brine shrimp, Artemia (Crustacea, Anostraca), is a zooplankton that is commonly used in both basic and applied research. Unfortunately, Artemia embryos are often cultured under conditions that alter early development, and reports based on these cultures oversimplify or fail to describe morphological phenotypes. This is due in part to the lack of a comprehensive developmental model that is applicable to observations of live specimens. The objective of this study was to build and test a descriptive model of post-diapause development in Artemia franciscana using observations made with a standard dissecting microscope. The working model presented is the first to comprehensively place all known "abnormal" embryonic and naupliar phenotypes within the context of a classic hatching profile. Contrary to previous reports, embryos and nauplii with aberrant phenotypes often recover and develop normally. Oval prenauplii may emerge as normal prenauplii (E2 stage). A delay of this transition leads to incomplete hatching or direct hatching of first instar larvae with a curved thoracoabdomen. When hatching is incomplete, retained cuticular remnants are shed during the next molt, and a "normal" second instar larva is produced. By differentiating between molting events and gross embryonic patterning in live embryos, this new model facilitates fine time-scale analyses of chemical and environmental impacts on early development. A small increase in salinity within what is commonly believed to be a permissive range (20‰-35‰) produced aberrant morphology by delaying emergence without slowing development. A similar effect was observed by decreasing culture density within a range commonly applied in toxicological studies. These findings clearly demonstrate that morphological data from end-point studies are highly dependent on the time points chosen. An alternate assessment method is proposed, and the potential impact of heavy metals, hexachlorobenzene, Mirex, and cis

  9. The immune-related fatty acids are responsive to CO2 driven seawater acidification in a crustacean brine shrimp Artemia sinica.

    Science.gov (United States)

    Gao, Yan; Zheng, Shu-Cheng; Zheng, Chao-Qun; Shi, Yue-Chen; Xie, Xiao-Lu; Wang, Ke-Jian; Liu, Hai-Peng

    2018-04-01

    The gradual increase of CO 2 concentration in the atmosphere, absorbed by the ocean surface water through air to sea equilibration termed ocean acidification (OA), leads to the decline of pH in seawater. It is not clear so far how the composition of fatty acids, particular the immune-related, in marine crustacean and the subsequent energy supply in marine ecosystem are affected by OA. The brine shrimp Artemia sinica is an open and common feed that provide essential fatty acids for mariculture. In this study, the fatty acids profiles of brine shrimp cultured under different lower pH levels of CO 2 driven seawater were investigated. The results showed a significant reduction of the proportion of total saturated fatty acids under the pH7.6 within one week. Meanwhile, the percentage of total monounsaturated fatty acids was significantly decreased at day 14 under pH7.8, and this percentage gave a significant increase of proportion within one week under pH7.6. Furthermore, the relative content of total polyunsaturated fatty acids (PUFAs) was found to be clearly increased with exposure to different seawater acidification at day 1, suggesting that the brine shrimp immune response was likely to be affected by acidified seawater as the PUFAs have been well known to be involved in immunomodulatory effects through alterations on cell membrane fluidity/lipid mediators and gene expression of cell signaling pathways. Notably, eicosapentaenoic acid and docosahexaenoic acid, which have essential effect on various physiological processes such as inflammatory cytokines production and cell structural stability, were strongly increased under two lower pH treatments within one week and with the significant increase at day 1 under pH7.6. These data clearly supported the hypothesis that OA might affect fatty acids composition, likely also the innate immunity, in crustacean and the subsequent energy transfer by food-chain system in the marine ecosystem. Copyright © 2017 Elsevier Ltd. All

  10. Survival of the probiotic bacteria Lactobacillus rhamnosus in seawater and its bioencapsulation in the brine shrimp Artemia

    Directory of Open Access Journals (Sweden)

    Claudia Ofelio

    2014-06-01

    The results obtained in the first test showed that the L. rhamnosus was able to survive in seawater during the whole experiment (30h, maintaining densities of 10e7 CFU/ml during the first 6h although decreasing progressively afterwards (10e3 CFU/ml at 30h. This allows adequate levels at sufficient time for Artemia to incorporate the probiotic. In fact, bioencapsulation test demonstrated that Artemia metanauplii were able to bioencapsulate the probiotic, reaching the highest concentration in Artemia after 30 min of bioencapsulation (10e4 CFU/Artemia. A slight further decrease (10e3 CFU/Artemia was observed after 24h. Interestingly, L. rhamnosus reduced in 1Log total Vibrionaceae bacteria in Artemia during the 3 first hours. Therefore, 3 hours was the time established for the bioencapsulation protocol and further studies are in progress to determine the ability of Artemia metanauplii to maintain bioencapsulated L. rhamnosus once transferred to rearing tanks. Also, the capability of the probiotic to inhibit potential pathogenic or opportunistic bacteria will be assessed.

  11. Effects of sonication and advanced chemical oxidants on the unicellular green alga Dunaliella tertiolecta and cysts, larvae and adults of the brine shrimp Artemia salina: a prospective treatment to eradicate invasive organisms from ballast water.

    Science.gov (United States)

    Gavand, Meghana R; McClintock, James B; Amsler, Charles D; Peters, Robert W; Angus, Robert A

    2007-11-01

    Uptake and release of ship-borne ballast water is a major factor contributing to introductions of aquatic phytoplankton and invasive macroinvertebrates. Some invasive unicellular algae can cause harmful algal blooms and produce toxins that build up in food chains. Moreover, to date, few studies have compared the efficacy of ballast water treatments against different life history phases of aquatic macroinvertebrates. In the present study, the unicellular green alga Dunaliella tertiolecta, and three discrete life history phases of the brine shrimp Artemia salina, were independently used as model organisms to study the efficacy of sonication as well as the advanced oxidants, hydrogen peroxide and ozone, as potential ballast water treatments. Algal cells and brine shrimp cysts, nauplii, and adults were subjected to individual and combined treatments of sonication and advanced oxidants. Combined rather than individual treatments consistently yielded the highest levels of mortality in algal cells (100% over a 2 min exposure) and in brine shrimp (100% and 95% for larvae and adults, respectively, over a 2 min exposure). In contrast, mortality levels in brine shrimp cysts (66% over 2 min; increased to 92% over a 20 min exposure) were moderately high but consistently lower than that detected for larval or adult shrimp. Our results indicate that a combination of sonication and advanced chemical oxidants may be a promising method to eradicate aquatic unicellular algae and macroinvertebrates in ballast water.

  12. Coadaptation: lessons from the brine shrimp Artemia, "the aquatic Drosophila" (Crustacea; Anostraca Coadaptación: lecciones desde el camarón de salmuera Artemia, "la Drosophila acuática" (Crustacea; Anostraca

    Directory of Open Access Journals (Sweden)

    GONZALO GAJARDO

    2001-03-01

    Full Text Available During the fifties Brncic and the Dobzhansky's school, using the fruit fly Drosophila as a test organism, introduced the term integration of the genotype, or genetic coadaptation, which has had great impact on thinking in today's evolutionary biology. In this work we use the brine shrimp Artemia - in many respects a sort of aquatic Drosophila- as a model organism to evaluate the relationship between population structure, potential for divergence and the degree of morphological and/or genetic change. These aspects, tightly linked with the organization of the genotype, are important to understanding how recombination and adaptive release of genetic and phenotypic variation affect the speciation process in Artemia. Analysis of genetic (allozyme, diploid and chromocentre numbers, morphological (Mahalanobis distances and reproductive data (cross-fertility tests available for populations of the bisexual, endemic species of the Americas, Artemia franciscana and A. persimilis, indicate that: (i A. franciscana and A. persimilis are morphologically distinct in correspondence with observed genetic differences (D Nei > 1; 2n = 42 and 44; 12.5 and 1.5 mean chromocentre numbers, respectively; (ii populations from Chile and other South American localities (mainly A. franciscana display high levels of genetic variability and a trend to develop large genetic distances between populations; (iii the plasticity of Artemia gene pool is associated, at least in part, with ecological heterogeneity. Hence an adaptive divergence mode is thought to best define the speciation process in Artemia; (iv the succesful production of laboratory hybrids in the allopatric Artemia populations studied in the Americas, a feature seen in other anostracods, could be explained by the fact that formerly allopatric populations have not achieved later sympatry, as required by the allopatric speciation paradigmEn los años cincuenta Brncic y la escuela de Dobzhansky introdujeron el t

  13. Proteomic analysis of acute responses to copper sulfate stress in larvae of the brine shrimp, Artemia sinica

    Science.gov (United States)

    Zhou, Qian; Wu, Changgong; Dong, Bo; Li, Fuhua; Liu, Fengqi; Xiang, Jianhai

    2010-03-01

    Proteomics was used to reveal the differential protein expression profiles of acute responses to copper sulfate exposure in larvae of Artemia sinica. Fourteen differentially displayed protein spots were detected and seven of them were identified. Three spots were up-expressed and identified: actin, heat shock protein 70, and chaperone subunit 1; three down-regulated proteins were identified: arginine kinase, elongation factor-2, and glycine-rich protein; and a newly expressed protein was identified as peroxiredoxin. The study indicates the involvement of all the differentially expressed proteins in the early responses of protein expression, and in the survival of A. sinica in the presence of copper and other heavy metals; the findings improve understanding of the organism’s adaptive responses and resistance.

  14. Sudden weaning of angel fish pterophyllum scalare (Lichtenstein) (Pisces; Cichlidae) larvae from brine shrimp (Artemia sp) nauplii to formulated larval feed.

    Science.gov (United States)

    Herath, Sandamali Sakunthala; Atapaththu, Kerthi Sri Senarathna

    2013-12-01

    This study investigated the effects of sudden weaning of angel fish larvae (Pteraphylum scalari) from Artemia nauplii to commercial larval feed. Four days post hatch (DPH) larvae were reared in four different weaning protocols (TR1-TR4) with triplicates in a complete randomize design. Larvae in TR1 and TR4 were exclusively fed Artemia nauplii and dry feed respectively. In TR2 and TR3, larvae were initially fed Artemia nauplii and suddenly wean to formulated feed on 14 DPH and 7 DPH respectively. The experiment was lasted for 28 days. At the end of the experiment, final mean weight (FW), total length (FL), height (FH), Daily Weight Gain (DWG), Specific Growth Rate (SGR), survival and stress index were compared. Significantly highest (P larvae solely fed formulated feed. Survival and the stress index were independent from weaning methods. Although sudden weaning is possible on 7 DPH, larvae showed comparatively higher growth when switch off to formulate feed on 14 DPH.

  15. An improved brine shrimp larvae lethality microwell test method.

    Science.gov (United States)

    Zhang, Yi; Mu, Jun; Han, Jinyuan; Gu, Xiaojie

    2012-01-01

    This article described an improved brine shrimp larvae lethality microwell test method. A simply designed connecting vessel with alternative photoperiod was used to culture and collect high yield of active Artemia parthenogenetica nauplii for brine shrimp larvae lethality microwell test. Using this method, pure A. parthenogenetica nauplii suspension was easily cultured and harvested with high density about 100-150 larvae per milliliter and the natural mortality was reduced to near zero by elimination of unnecessary artificial disturbance. And its sensitivity was validated by determination of LC(50)-24 h of different reference toxicants including five antitumor agents, two pesticides, three organic pollutants, and four heavy metals salts, most of which exhibited LC(50)-24 h between 0.07 and 58.43 mg/L except for bleomycin and mitomycin C with LC(50)-24 h over 300 mg/L.

  16. Accumulation of pesticide residues by shrimp, fish and brine shrimp during pond culture at Ghorabari (District Thatta)

    International Nuclear Information System (INIS)

    Sultana, R.; Wajeeha, F.A.; Ameer, F.; Munshi, A.B.; Nasir, M.

    2012-01-01

    Residual level of persistent organo chlorines (OC) such as sigma-HCH (alpha-HCH, beta-HCH, gamma-HCH, delta-HCH, sigma-DDT (o,p'-DDD, op-DDE, p,p-DDE pp-DDD, pp'-DDT, o,p'-DDT), dieldrin and endrin were measured in a number of water samples from Ambro creek and their accumulation in shrimp (Penaeus merguiensis and P. penicillatus), fish (Otolithes ruber) and brine shrimp (Artemia sp.) reared in ponds for a period of four months. Samples were extracted with organic solvents, and quantified using gas chromatography-electron capture detection (GC/ECD). It has been found that results of animal tissue and water are not same however OCs, (mainly sigma DT and beta-HCH 4,4-DDT, Dieldrin + 2,4-DDT, and Methoxychlor were detected in all samples ). Heptachlor exo-epoxide were found in fish and Artemia sp. and absent in all shrimp samples. Heptachlor endo-epoxide was detected only in Artemia sp. and average residual concentration of OCs in Artemia sp. was 0.004-0.09 ppm. Methoxychlor was found in the highest quantity in all the samples whether it was fish, shrimp or Artemia. In fish average residual concentration of all (OCs) in individual sample was 0.03 - 0.180 ppm. (author)

  17. Comparison live adult Artemia and squid meat on the growth of Penaeid shrimp Metapenaeus dobsoni (Miers)

    Digital Repository Service at National Institute of Oceanography (India)

    Nair, S.R.S.; Achuthankutty, C.T.; Royan, J.P.

    An experiment lasting 14 weeks was conducted to compare the efficiency of live adult Artemia with fresh squid meat on growth of penaeid shrimp Metapenaeus dobsoni. The shrimps were found actively feeding on live Artemia and grew 84% more than those...

  18. Prospects of Artemia culture in the coastal zone

    Digital Repository Service at National Institute of Oceanography (India)

    Royan, J.P.

    The importance of the brine shrimp Artemia as live feed in shrimp hatcheries has been pointed out. The paper deals with the resource potential and status of cyst production in India. There is a need for setting up a Pilot Project for Artemia cyst...

  19. Composição química, atividade antibacteriana in vitro e toxicidade em Artemia salina do óleo essencial das inflorescências de Ocimum gratissimum L., Lamiaceae Chemical composition, antibacterial activity in vitro and brine-shrimp toxicity of the essential oil from inflorescences of Ocimum gratissimum L., Lamiaceae.

    Directory of Open Access Journals (Sweden)

    Lenise L. Silva

    2010-11-01

    Full Text Available O óleo essencial das inflorescências de Ocimum gratissimum L., Lamiaceae foi obtido por hidrodestilação e analisado por CG/EM. Os constituintes majoritários identificados foram eugenol (81,94% e γ-muuroleno (12,58%. O óleo essencial das inflorescências demonstrou atividade antibacteriana frente a todas as cepas bacterianas testadas pelo método de microdiluição em caldo. Merece destaque a atividade verificada frente às cepas resistentes de Enterococcus faecalis, Staphylococcus aureus e Escherichia coli. Os valores obtidos de concentração inibitória mínima (CIM e a concentração bactericida mínima (CBM variaram, respectivamente, entre 0,5-2 mg/mL e 1-4 mg/mL. Valores de CL50 de 233,8 (200,7-272,0 µg/mL para o óleo essencial e 186,1 (144,1-228,5 µg/mL para o eugenol, utilizado como controle positivo, foram observados frente à Artemia salina L.The essential oil obtained by hydrodistillation of the inflorescences of Ocimum gratissimum L. was analyzed by GC/MS. The main constituents were eugenol (81.94% and γ-muurolene (12.58%. Antibacterial activity was shown against all assayed strains by the broth microdilution method. It's worth noting the activity against resistant strains of Enterococcus faecalis, Staphylococcus aureus and Escherichia coli. Minimum inhibitory concentration (MIC and minimum bactericidal concentration (MBC values ranged between 0.5-2 mg/mL and 1-4 mg/mL, respectively. Preliminary toxicity assayed by the brine-shrimp (Artemia salina L. test showed LC50 values of 233.8 (200.7 - 272.0 µg/mL and 186.1 (144.1 - 228.5 µg/mL, respectively for the essential oil and eugenol (positive control.

  20. Brine Shrimp Toxicity Evaluation Of Some Tanzanian Plants Used ...

    African Journals Online (AJOL)

    Plants which are used by traditional healers in Tanzania have been evaluated to obtain preliminary data of their toxicity using the brine shrimps test. The results indicate that 9 out of 44 plant species whose extracts were tested exhibited high toxicity with LC50 values below 20μg/ml. These include Aloe lateritia Engl.

  1. Brine shrimp cytotoxicity of Caesalpinia pulcherrima aerial parts, antimicrobial activity and characterisation of isolated active fractions.

    Science.gov (United States)

    Chanda, Sumitra; Baravalia, Yogesh

    2011-12-01

    Caesalpinia pulcherrima Swartz. is an ornamental plant, shrub or a small tree belonging to the family Caesalpiniaceae. The plant has been used for the treatment of inflammatory disorders, skin diseases and so on. In this study, the cytotoxicity of the methanol extract of the aerial parts of C. pulcherrima was tested using an Artemia salina (brine shrimp) bioassay. Further, the methanol extract was fractionated by silica gel column chromatography using a solvent gradient of hexane:ethyl acetate:methanol in different ratios and 56 fractions were collected. On the basis of thin layer chromatography profiles, 13 major fractions were obtained, which were tested for antimicrobial activity against 14 microorganisms using the agar disc diffusion method and also tested for their minimal inhibitory concentration and minimal bactericidal concentration values. In terms of cytotoxicity, the extract caused 26% mortality of brine shrimp larvae after 24 h at a concentration of 1000 µg mL(-1). Fractions 3, 9 and 10 showed significant antimicrobial activities. Phytochemical analysis of these three fractions led to the identification of 11 compounds, and their structures were established by means of gas chromatography-mass spectroscopy techniques. These findings suggest that these bioactive compounds may be useful as potential antimicrobials. Further investigation is needed to establish the mode of action of these bioactive compounds.

  2. Pengujian Fitokimia dan Toksisitas Ekstrak Etanol Jantung Pisang Kepok (Musa paradisiaca LINN. dengan Metode Brine Shrimp Lethality Test (BSLT

    Directory of Open Access Journals (Sweden)

    Meytij Jeanne Rampe

    2016-05-01

    Full Text Available Telah dilakukan pengujian fitokimia dan toksisitas ekstrak etanol jantung pisang kepok (Musa Paradisiaca Linn. dengan metode Brine Shrimp Lethality Test (BSLT.  Pengujian dilakukan untuk mencari beberapa senyawa kimia dari ekstrak jantung pisang Musa Paradisaca. Linn serta mengetahui efek toksik terhadap larva udang Artemia salina. Leach.  Maserasi jantung pisang kepok dilakukan dengan menggunakan etanol. Ekstrak etanol yang diperoleh dilakukan pengujian fitokimia dan toksisitas. Hasil pengujian fitokimia menunjukkan ekstrak etanol jantung pisang Musa paradisiaca. Linn memiliki komposisi senyawa flavonoid, kumarin dan senyawa fenolik lainnya. Ekstrak etanol jantung pisang kepok memberikan efek toksik terhadap larva udang Artemia salina. Leach dengan nilai LC50 sebesar 806,8 μg/mL.Kata kunci: jantung pisang kepok, fitokimia, toksisitas, BSLT.

  3. Cyst quality and hatching in parthenogenetic brine shrimp, Artemia

    Digital Repository Service at National Institute of Oceanography (India)

    Royan, J.P.; Sumitra-Vijayaraghavan; Krishnakumari, L.; Ramaiah, N.

    ) enhance the percentage of hatching of the cysts. On the basis of naupliar size, low ash content and high protein content it can be suggested that this strain is likely to be preferred by predators...

  4. Toxicity of heavy metals to brine shrimp Artemia

    Digital Repository Service at National Institute of Oceanography (India)

    Gajbhiye, S.N.; Hirota, R.

    stream_size 8 stream_content_type text/plain stream_name J_Indian_Fish_Assoc_20_43.pdf.txt stream_source_info J_Indian_Fish_Assoc_20_43.pdf.txt Content-Encoding ISO-8859-1 Content-Type text/plain; charset=ISO-8859-1 ...

  5. Microbial changes and growth of Listeria monocytogenes during chilled storage of brined shrimp ( Pandalus borealis )

    DEFF Research Database (Denmark)

    Mejlholm, Ole; Kjeldgaard, J.; Modberg, A.

    2008-01-01

    Thirteen storage trials and ten challenge tests were carried out to examine microbial changes, spoilage and the potential growth of Listeria monocytogenes in brined shrimp (Pandalus borealis). Shrimp in brine as well as brined and drained shrimp in modified atmosphere packaging (MAP) were produced...... and lactic acids were studied. Furthermore, the effect of adding diacetate to brined shrimp was evaluated. A single batch of cooked and peeled shrimp was used to study both industrially and manually processed brined shrimp with respect to the effect of process hygiene on microbial changes and the shelf life...... of products. Concentrations of microorganisms on newly produced brined shrimp from an industrial scale processing line were 1.0-2.3 log (CFU g(-1)) higher than comparable concentrations in manually processed samples. This resulted in a substantially shorter shelf life and a more diverse spoilage microflora...

  6. Use of Probiotic Bacillus spp. in Rotifer (Brachionus plicatilis) and Artemia (Artemia urmiana) Enrichment: Effects on Growth and Survival of Pacific White Shrimp, Litopenaeus vannamei, Larvae.

    Science.gov (United States)

    Jamali, Hadi; Imani, Ahmad; Abdollahi, Daruosh; Roozbehfar, Reza; Isari, Amin

    2015-06-01

    This study was to evaluate the effect of a preparation of Bacillus probiotic (Bacillus licheniformis and B. subtilis, 1:1) on growth and survival rate of Pacific white shrimp, Litopenaeus vannamei larvae. The larvae were fed on Artemia urmiana nauplii and Brachionus plicatilis enriched with the probiotic preparation at 1 × 10(6) CFU mL(-1) rate. The experimental setup was completely randomized design comprised of six treatments, namely solo Artemia nauplii (A) or rotifer (R), Artemia nauplii and rotifer without any enrichment (A + R), Artemia nauplii enrichment with probiotic bacilli (Bacillus licheniformis and B. subtilis) (A + B), rotifer enrichment with probiotic bacilli (R + B) and enriched Artemia nauplii and rotifer (A + R + B). All treatments were performed in triplicate. Chemical parameters of rearing water viz. pH, salinity and temperature were 7.5-8, 30-31 ppt and 31-32 °C, respectively. Photoperiod was 16L:8D. Shrimp larvae were fed Artemia nauplii and rotifers at 5-20 and 10-40 individuals per shrimp larvae four times a day, respectively. Growth and survival rate of larvae were determined at MII, MIII, PL1, PL4, PL7 and PL10 stages. Larvae in A + R + B treatment showed the highest total length (10.89 ± 0.51 mm), weight (674 ± 73 μg) and survival rate (65% ± 3.5). Lowest total length, weight and survival rate (7.96 ± 0.63 mm, 493 ± 52 μg and 24.5 ± 2.4%, respectively) were recorded in treatment B larvae. We concluded that Bacillus probiotic can improve growth and survival rate of Pacific white shrimp larvae without conceivably undesirable effects.

  7. Monitoring of Vibrio harveyi quorum sensing activity in real time during infection of brine shrimp larvae.

    Science.gov (United States)

    Defoirdt, Tom; Sorgeloos, Patrick

    2012-12-01

    Quorum sensing, bacterial cell-to-cell communication, has been linked to the virulence of pathogenic bacteria. Indeed, in vitro experiments have shown that many bacterial pathogens regulate the expression of virulence genes by this cell-to-cell communication process. Moreover, signal molecules have been detected in samples retrieved from infected hosts and quorum sensing disruption has been reported to result in reduced virulence in different host-pathogen systems. However, data on in vivo quorum sensing activity of pathogens during infection of a host are currently lacking. We previously reported that quorum sensing regulates the virulence of Vibrio harveyi in a standardised model system with gnotobiotic brine shrimp (Artemia franciscana) larvae. Here, we monitored quorum sensing activity in Vibrio harveyi during infection of the shrimp, using bioluminescence as a read-out. We found that wild-type Vibrio harveyi shows a strong increase in quorum sensing activity early during infection. In this respect, the bacteria behave remarkably similar in different larvae, despite the fact that only half of them survive the infection. Interestingly, when expressed per bacterial cell, Vibrio harveyi showed around 200-fold higher maximal quorum sensing-regulated bioluminescence when associated with larvae than in the culture water. Finally, the in vivo quorum sensing activity of mutants defective in the production of one of the three signal molecules is consistent with their virulence, with no detectable in vivo quorum sensing activity in AI-2- and CAI-1-deficient mutants. These results indicate that AI-2 and CAI-1 are the dominant signals during infection of brine shrimp.

  8. Guiding brine shrimp through mazes by solving reaction diffusion equations

    Science.gov (United States)

    Singal, Krishma; Fenton, Flavio

    Excitable systems driven by reaction diffusion equations have been shown to not only find solutions to mazes but to also to find the shortest path between the beginning and the end of the maze. In this talk we describe how we can use the Fitzhugh-Nagumo model, a generic model for excitable media, to solve a maze by varying the basin of attraction of its two fixed points. We demonstrate how two dimensional mazes are solved numerically using a Java Applet and then accelerated to run in real time by using graphic processors (GPUs). An application of this work is shown by guiding phototactic brine shrimp through a maze solved by the algorithm. Once the path is obtained, an Arduino directs the shrimp through the maze using lights from LEDs placed at the floor of the Maze. This method running in real time could be eventually used for guiding robots and cars through traffic.

  9. Scanning electron microscope observations of brine shrimp larvae from space shuttle experiments

    Science.gov (United States)

    DeBell, L.; Paulsen, A.; Spooner, B.

    1992-01-01

    Brine shrimp are encysted as gastrula stage embryos, and may remain dehydrated and encysted for years without compromising their viability. This aspect of brine shrimp biology is desirable for studying development of animals during space shuttle flight, as cysts placed aboard a spacecraft may be rehydrated at the convenience of an astronaut, guaranteeing that subsequent brine shrimp development occurs only on orbit and not on the pad during launch delays. Brine shrimp cysts placed in 5 ml syringes were rehydrated with salt water and hatched during a 9 day space shuttle mission. Subsequent larvae developed to the 8th larval stage in the sealed syringes. We studied the morphogenesis of the brine shrimp larvae and found the larvae from the space shuttle experiments similar in rate of growth and extent of development, to larvae grown in sealed syringes on the ground. Extensive differentiation and development of embryos and larvae can occur in a microgravity environment.

  10. Alternative mitochondrial respiratory chains from two crustaceans: Artemia franciscana nauplii and the white shrimp, Litopenaeus vannamei.

    Science.gov (United States)

    Rodriguez-Armenta, Chrystian; Uribe-Carvajal, Salvador; Rosas-Lemus, Monica; Chiquete-Felix, Natalia; Huerta-Ocampo, Jose Angel; Muhlia-Almazan, Adriana

    2018-04-01

    Mitochondrial ATP is synthesized by coupling between the electron transport chain and complex V. In contrast, physiological uncoupling of these processes allows mitochondria to consume oxygen at high rates without ATP synthesis. Such uncoupling mechanisms prevent reactive oxygen species overproduction. One of these mechanisms are the alternative redox enzymes from the mitochondrial respiratory chain, which may help cells to maintain homeostasis under stress independently of ATP synthesis. To date, no reports have been published on alternative redox enzymes in crustaceans mitochondria. Specific inhibitors were used to identify alternative redox enzymes in mitochondria isolated from Artemia franciscana nauplii, and the white shrimp, Litopenaeus vannamei. We report the presence of two alternative redox enzymes in the respiratory chain of A. franciscana nauplii, whose isolated mitochondria used glycerol-3-phosphate as a substrate, suggesting the existence of a glycerol-3-phosphate dehydrogenase. In addition, cyanide and octyl-gallate were necessary to fully inhibit this species' mitochondrial oxygen consumption, suggesting an alternative oxidase is present. The in-gel activity analysis confirmed that additional mitochondrial redox proteins exist in A. franciscana. A mitochondrial glycerol-3-phosphate dehydrogenase oxidase was identified by protein sequencing as part of a branched respiratory chain, and an alternative oxidase was also identified in this species by western blot. These results indicate different adaptive mechanisms from artemia to face environmental challenges related to the changing levels of oxygen concentration in seawater through their life cycles. No alternative redox enzymes were found in shrimp mitochondria, further efforts will determine the existence of an uncoupling mechanism such as uncoupling proteins.

  11. BRINE SHRIMP LETHALITY TEST (BSLT DARI BERBAGAI FRAKSI EKSTRAK DAGING BUAH DAN KULIT BIJI MAHKOTA DEWA (Phaleria macrocarpa

    Directory of Open Access Journals (Sweden)

    Vivi Lisdawati

    2012-10-01

    Full Text Available Biological activity of a natural product involved in several certain characteristics will influence its pharmaceutical application. Secondary metabolites, considered as chemical compounds, are now thought to mediate plant defense mechanism by providing chemical barriers against animal and microbial predators. Brine Shrimp Lethality Test (BSLT method has been used as preliminary test for screening the activity of chemical compounds in n­ hexane, ethyl acetate, and methanol extracts from mesocarp and seeds of Phaleria macrocarpa, fam. Thymelaeaceae. BSLT method used shrimp larvas of Artemia salina L. to study the mortality effect that was caused by the sample extracts. All of crude extracts showed bioactivity with LC50 values from 0.16 to 11.83 µg/ml (baseline 1000 µg/ml. It means, at the concentration the crude extracts can cause 50% mortality of A. salina L. shrimp larvas, after 24 hours incubation. These results clearly indicate that crude extracts of P. macrocarpa showed high potential biological activity.

  12. Brine shrimp lethality and antibacterial activity of extracts from the bark of Schleichera oleosa

    Directory of Open Access Journals (Sweden)

    Laxman Pokhrel

    2015-08-01

    Full Text Available Objective: To determine the antibacterial efficacy and brine shrimp toxicity of extracts (hexane, dichloromethane, ethyl acetate, methanol and water obtained from the bark of Schleichera oleosa. Methods: The powdered bark sample was Soxhlet extracted sequentially in hexanes, dichloromethane, ethyl acetate, methanol and water. Antibacterial evaluation was carried out by following the agar diffusion method and amoxicillin disc was used as a reference. Slightly modified Meyer’s method was used to determine the toxicity of the extracts in brine shrimps. Results: Among the nine bacterial strains tested, the methanolic and aqueous extracts showed promising antibacterial efficacy against Serratia marcescens, Escherarichia coli, Bacillus subtilis and Micrococcus luteus. None of the extracts were found significantly toxic to brine shrimps. Conclusions: Strong antibacterial activity and low brine shrimp toxicity of methanolic and aqueous extracts can provide new antibacterial compounds.

  13. Use of the genus Artemia in ecotoxicity testing

    International Nuclear Information System (INIS)

    Nunes, Bruno S.; Carvalho, Felix D.; Guilhermino, Lucia M.; Van Stappen, Gilbert

    2006-01-01

    Information related to varied uses of several species of the genus Artemia (commonly known as brine shrimp), is dispersed among literature from several scientific areas, such as Ecology, Physiology, Ecotoxicology, Aquaculture and Genetics. The present paper reviews information related to Artemia that may be considered relevant for ecotoxicity testing. Integration of different areas of scientific knowledge concerning biology, life cycle and environmental needs of Artemia is of crucial importance when considering the interpretation of results drawn from tests involving this genus. Furthermore, this paper provides suggestions to overcome problems related to toxicity assessment with the use of Artemia as test organism in bioassays, under the scope of estuarine, marine and hypersaline environments. Aspects related to variability in results, adoptable toxicity end-points, culture conditions, characteristics of species and strains, influence of geographical origins over physiological features and responses to exposure to chemical agents are considered. - The physiology, reproductive processes and general use of Artemia in modern ecotoxicological testing are reviewed

  14. Use of the genus Artemia in ecotoxicity testing

    Energy Technology Data Exchange (ETDEWEB)

    Nunes, Bruno S. [Instituto de Ciencias Biomedicas de Abel Salazar, Departamento de Estudos de Populacoes, Laboratorio de Ecotoxicologia, Universidade do Porto, Largo Prof. Abel Salazar 2, 4099-003 Porto (Portugal) and Centro Interdisciplinar de Investigacao Marinha e Ambiental, Rua dos Bragas 289, 4150-123 Porto (Portugal)]. E-mail: bruno@ufp.pt; Carvalho, Felix D. [REQUIMTE, Departamento de Toxicologia da Faculdade de Farmacia da Universidade do Porto, Rua Anibal Cunha, 164, 4050-047 Porto (Portugal); Guilhermino, Lucia M. [Instituto de Ciencias Biomedicas de Abel Salazar, Departamento de Estudos de Populacoes, Laboratorio de Ecotoxicologia, Universidade do Porto, Largo Prof. Abel Salazar 2, 4099-003 Porto (Portugal); Centro Interdisciplinar de Investigacao Marinha e Ambiental, Rua dos Bragas 289, 4150-123 Porto (Portugal); Van Stappen, Gilbert [Laboratory of Aquaculture and Artemia Reference Centre, Ghent University, Rozier 44, B-9000 Ghent (Belgium)

    2006-11-15

    Information related to varied uses of several species of the genus Artemia (commonly known as brine shrimp), is dispersed among literature from several scientific areas, such as Ecology, Physiology, Ecotoxicology, Aquaculture and Genetics. The present paper reviews information related to Artemia that may be considered relevant for ecotoxicity testing. Integration of different areas of scientific knowledge concerning biology, life cycle and environmental needs of Artemia is of crucial importance when considering the interpretation of results drawn from tests involving this genus. Furthermore, this paper provides suggestions to overcome problems related to toxicity assessment with the use of Artemia as test organism in bioassays, under the scope of estuarine, marine and hypersaline environments. Aspects related to variability in results, adoptable toxicity end-points, culture conditions, characteristics of species and strains, influence of geographical origins over physiological features and responses to exposure to chemical agents are considered. - The physiology, reproductive processes and general use of Artemia in modern ecotoxicological testing are reviewed.

  15. An investigation on the application of ohmic heating of cold water shrimp and brine mixtures

    DEFF Research Database (Denmark)

    Pedersen, Søren Juhl; Feyissa, Aberham Hailu; Brøkner Kavli, Sissel Therese

    2016-01-01

    Cooking is an important unit-operation in the production of cooked and peeled shrimps. The present study explores the feasibility of using ohmic heating for cooking of shrimps. The focus is on investigating the effects of different process parameters on heating time and quality of ohmic cooked...... shrimps (Pandalus Borelias). The shrimps were heated to a core temperature of 72 °C in a brine solution using a small batch ohmic heater. Three experiments were performed: 1) a comparative analyses of the temperature development between different sizes of shrimps and thickness (head and tail region...... of the shrimp) over varying salt concentrations (10 kg m−3 to 20 kg m−3) and electric field strengths (1150 V m−1 to 1725 V m−1) with the heating time as the response; 2) a 2 level factorial experiment for screening the impact of processing conditions using electric field strengths of 1250 V m−1 and 1580 V m−1...

  16. Brine shrimp toxicity and antimalarial activity of some plants traditionally used in treatment of malaria in Msambweni district of Kenya.

    Science.gov (United States)

    Nguta, J M; Mbaria, J M

    2013-07-30

    phytoconstituents since the assayed plant parts are used in combination with others to treat malaria. It is also evident that none of the screened plant extracts is toxic to the arthropod invertebrate, Artemia salina L. (Artemiidae) larvae, justifying the continued use of the plant parts to treat malaria. A.seyal, G.trichocarpa and T.indica have not been reported before for in vivo antimalarial activity and brine shrimp lethality. Copyright © 2013 Elsevier Ireland Ltd. All rights reserved.

  17. Decapsulated brine shrimp cysts - an ideal feed for shrimps in aquaculture

    Digital Repository Service at National Institute of Oceanography (India)

    Royan, J.P.

    Feeding experiments have been carried out with Metapenaeus monoceros using different stages of Artemia salina as feed. The importance of using decapsulated cysts to obtain greater food conversion efficiency has been indicated...

  18. Phloroglucinol-Mediated Hsp70 Production in Crustaceans: Protection against Vibrio parahaemolyticus in Artemia franciscana and Macrobrachium rosenbergii

    Science.gov (United States)

    Kumar, Vikash; Baruah, Kartik; Nguyen, Dung Viet; Smagghe, Guy; Vossen, Els; Bossier, Peter

    2018-01-01

    The halophilic aquatic bacterium, Vibrio parahaemolyticus, is an important aquatic pathogen, also capable of causing acute hepatopancreatic necrosis disease (AHPND) in shrimp resulting in significant economic losses. Therefore, there is an urgent need to develop anti-infective strategies to control AHPND. The gnotobiotic Artemia model is used to establish whether a phenolic compound phloroglucinol is effective against the AHPND strain V. parahaemolyticus MO904. We found that pretreatment with phloroglucinol, at an optimum concentration (30 µM), protects axenic brine shrimp larvae against V. parahaemolyticus infection and induced heat shock protein 70 (Hsp70) production (twofolds or more) as compared with the control. We further demonstrated that the Vibrio-protective effect of phloroglucinol was caused by its prooxidant effect and is linked to the induction of Hsp70. In addition, RNA interference confirms that phloroglucinol-induced Hsp70 mediates the survival of brine shrimp larvae against V. parahaemolyticus infection. The study was validated in xenic Artemia model and in a Macrobrachium rosenbergii system. Pretreatment of xenic brine shrimp larvae (30 µM) and Macrobrachium larvae (5 µM) with phloroglucinol increases the survival of xenic brine shrimp and Macrobrachium larvae against subsequent V. parahaemolyticus challenge. Taken together, our study provides substantial evidence that the prooxidant activity of phloroglucinol induces Hsp70 production protecting brine shrimp, A. franciscana, and freshwater shrimp, M. rosenbergii, against the AHPND V. parahaemolyticus strain MO904. Probably, phloroglucinol treatment might become part of a holistic strategy to control AHPND in shrimp.

  19. Improvement in Shrimp Hatchery Procedures for Toxicity Tests

    International Nuclear Information System (INIS)

    Nor Azizah Marsiddi; Fazliana Mohd Saaya; Anee Suryani Sued

    2015-01-01

    Toxicity testing of brine shrimp Artemia salina Brine shrimp lethality assay is a screening test to determine half the dose mortality (LC50) for its shrimp given certain herbal extract at a concentration tested. The shrimp child mortality half a dose indicator to determine level of toxicity before further testing done on animal cell culture and animal experiments also on the mouse. The use of new hardware, namely Artemio 1 has increased its shrimp production at a rate that more and faster than the use of the black box hatching previously taken from the method by Solis, 1993. brine shrimp eggs from Artemio mix also easier to use because it contains egg and sea salt have been ready mixed for use in experiments. In conclusion, this method improvements help increase the number of offspring produced shrimp and produce experimental method easier than previous methods. (author)

  20. A method for the specific detection of resident bacteria in brine shrimp larvae.

    Science.gov (United States)

    Niu, Yufeng; Defoirdt, Tom; Rekecki, Anamaria; De Schryver, Peter; Van den Broeck, Wim; Dong, Shuanglin; Sorgeloos, Patrick; Boon, Nico; Bossier, Peter

    2012-04-01

    In this study, we describe an easy but efficient method to specifically target the intestinal resident microbiota in brine shrimp larvae during DGGE analysis, hereby excluding the interference of both transient (luminal) bacteria and body surface bacteria. This effective technique has several advantages over alternative methods, with respect of ease of use and rapidity. Copyright © 2012 Elsevier B.V. All rights reserved.

  1. Reducing Vibrio load in Artemia nauplii using antimicrobial photodynamic therapy: a promising strategy to reduce antibiotic application in shrimp larviculture

    Science.gov (United States)

    Asok, Aparna; Arshad, Esha; Jasmin, C.; Somnath Pai, S.; Bright Singh, I. S.; Mohandas, A.; Anas, Abdulaziz

    2012-01-01

    Summary We propose antimicrobial photodynamic therapy (aPDT) as an alternative strategy to reduce the use of antibiotics in shrimp larviculture systems. The growth of a multiple antibiotic resistant Vibrio harveyi strain was effectively controlled by treating the cells with Rose Bengal and photosensitizing for 30 min using a halogen lamp. This resulted in the death of > 50% of the cells within the first 10 min of exposure and the 50% reduction in the cell wall integrity after 30 min could be attributed to the destruction of outer membrane protein of V. harveyi by reactive oxygen intermediates produced during the photosensitization. Further, mesocosm experiments with V. harveyi and Artemia nauplii demonstrated that in 30 min, the aPDT could kill 78.9% and 91.2% of heterotrophic bacterial and Vibrio population respectively. In conclusion, the study demonstrated that aPDT with its rapid action and as yet unreported resistance development possibilities could be a propitious strategy to reduce the use of antibiotics in shrimp larviculture systems and thereby, avoid their hazardous effects on human health and the ecosystem at large. PMID:21951316

  2. BRINE SHRIMP LETHALITY BIOASSAY OF GLAUCIUM GRANDIFLORUM VAR. GRANDIFLORUM

    OpenAIRE

    A. SARI, Ç. ÜNSAL, İ. SARIOĞLU, A. SARI, Ç. ÜNSAL, İ. SARIOĞLU

    2013-01-01

    Türkiye'nin 3 farklı bölgesinden toplanan Glaucium grandiflorum Boiss. et Huet var. grandiflorum örneklerinin toprak üstü kısımlarından elde edilen alkaloit ekstreleri ve bu ekstrelerden elde edilen majör alkaloitler allokriptopin, protopİn, (+)-izokoridin, (+)-korİdin üzerinde brİne shrimp lethality testi yapılarak sitotoksisiteleri İncelenmiştir. Glaucium grandiflorum var. grandiflorum türünün 3 örneği de önemli oranda sitotoksik aktİvite göstermiştir. Allokriptopin, protopin, (+)-izok...

  3. Fine structure and function of the alimentary epithelium in Artemia salina nauplii

    Energy Technology Data Exchange (ETDEWEB)

    Hootman, S R; Conte, F P

    1974-01-01

    The fine structure of the alimentary tract in the second instar nauplius of the brine shrimp, Artemia salina, has been described. The foregut and hindgut of the larva are composed of cuboidal epithelium which is cuticularized. The epithelium of the midgut and gastric caeca is columnar and is characterized by apical microvilli, basal membrane infolds, and abundant mitochondria. The structural characteristics of the midgut cells correlate with previous physiological and biochemical evidence on both adult and larval brine shrimp which indicates that the midgut plays an important role in absorption and osmoregulation in these animals.

  4. Nano-sized polystyrene affects feeding, behavior and physiology of brine shrimp Artemia franciscana larvae

    NARCIS (Netherlands)

    Bergami, Elisa; Bocci, Elena; Vannuccini, Maria Luisa; Monopoli, Marco; Salvati, Anna; Dawson, Kenneth A; Corsi, Ilaria

    Nano-sized polymers as polystyrene (PS) constitute one of the main challenges for marine ecosystems, since they can distribute along the whole water column affecting planktonic species and consequently disrupting the energy flow of marine ecosystems. Nowadays very little knowledge is available on

  5. Phytochemistry, Brine shrimp lethality and mice acute oral toxicity studies on seed extracts of Vernonia anthelmintica.

    Science.gov (United States)

    Jamil, Subia; Khan, Rafeeq Alam; Afroz, Syeda; Ahmed, Shadab

    2016-11-01

    Despite the widespread use of Vernonia anthelmintica seeds in traditional medicine, the need to establish the safety of the Vernonia anthelmintica is required to ascertain the safe use of this herbal medicine. The aim of the present study is to establish the acute toxicity profile of different extracts of Vernonia anthelmintica. Hexane and ethanol extract of Vernonia Anthelmintica has been studied for its brine shrimp lethality potential. Water decoction (WDVA), Hexane (HEVA) and Ethanol (EEVA) extracts of Vernonia anthelmintica has also been evaluated for their in-vivo acute oral toxicity in mice by Lorke's method. Phytochemistry of all three extracts was also evaluated for the presence of their secondary metabolites. All three extracts showed the presence of flavonoids and terpenoids, while alkaloids, tannins and fixed oils were present in HEVA and EEVA. Furthermore EEVA also showed presence of carbohydrates and HEVA also showed the presence of cardiac glycosides. Ethanol and hexane extracts of Vernonia anthelmintica showed a positive cytotoxicity in brine shrimp lethality test at 24 hours with LC50 104.16 (224.0-48.05)μg/ml and 216.11μg/ml (378.2-128.7) respectively as compared to standard drug etoposide LC50 7.46μg/ml. The oral LD50 for EEVA, HEVA and WDVA in mice by Lorke's method was greater than 5000mg/kg. The result of brine shrimp lethality test clearly exhibited the presence of bioactive compounds with cytotoxic potential; however seems to be safe for oral use since LD50 was higher than 5000mg/kg and thus safety of acute dosing in vivo practices is justified.

  6. Chemical Composition and Enzymes Inhibitory, Brine Shrimp Larvae Toxicity, Antimicrobial and Antioxidant Activities of Caloplaca biatorina

    Directory of Open Access Journals (Sweden)

    Tahereh Valadbeigi

    2016-10-01

    Full Text Available Background This study evaluated the brine shrimp larvae toxicity and enzymes inhibitory especially anti-diabetic potential of Caloplaca biatorina via in vitro inhibition of α-amylase and α-glucosidase using the methanol extracts. Also aldehyde oxidase and xanthine oxidase enzymes inhibitory, cytotoxicity, and antioxidant activities of the species were determined. Methods In this experimental study, different concentrations of the extracts (0.2, 5.0, 1 and 1.5 mg/mL were incubated with enzyme substrate solution and the percentage of enzyme inhibitory activity and IC50 was calculated. Folin- Ciocalteu reagent and aluminium chloride colorimetric methods were used to estimate total phenolic and flavonoid content of extracts. The toxicity of the extract was assessed using the brine shrimp lethality bioassay. The minimal inhibitory concentration (MIC and minimum bactericidal concentration (MBC were determined. High-performance liquid chromatography and Thin-layer chromatography analysis were evaluated. The data were analyzed by SPSS V.21 software. Results Parietin, Emodin, 1,8-Dihydroxy-3-(hydroxymethyl-6- methoxy-9.10-anthracenedione and Rhein were identified. The extract showed strong α-glucosidase, aldehyde oxidase and xanthine oxidase inhibitory activities with IC50 value of 17.12, 40.09 and 11.02 µg/mL respectively. Also methanol extract displayed the strongest DPPH radical scavenging and brine shrimp toxicity (IC50 = 91.11 properties. Conclusions The result obtained suggests that the C. biatorina extract can be classified as non-toxic. Also, it revealed the antioxidant and antidiabetic potential of the lichen.

  7. Evaluation of larvicidal activity and brine shrimp toxicity of rhizome extracts of Zingiber zerumbet (L.) Smith.

    Science.gov (United States)

    Bücker, Augusto; Falcão-Bücker, Nádia Cristina; Nunez, Cecília Veronica; Pinheiro, Carlos Cleomir de Souza; Tadei, Wanderli Pedro

    2013-01-01

    In this study, we used dichloromethane (DCM) and methanol (MeOH) extracts of the Zingiber zerumbet rhizome to evaluate brine shrimp lethality and larvicidal activity on Aedes aegypti and Anopheles nuneztovari mosquitoes. Bioassays were performed by exposing third-instar larvae of each mosquito species to the DCM or MeOH extracts. Probit analysis with DCM and MeOH extracts demonstrated efficient larvicidal activity against A. aegypti and A. nuneztovari larvae. The DCM and MeOH extracts showed higher activity against A. nuneztovari larvae than against A. aegypti larvae, suggesting that the extracts have species-specific activity.

  8. Anti-Candida activity and brine shrimp toxicity assay of Ganoderma boninense.

    Science.gov (United States)

    Daruliza, K M A; Fernandez, L; Jegathambigai, R; Sasidharan, S

    2012-01-01

    Ganoderma (G.) boninense is a white rot fungus, which can be found in the palm oil tree. Several studies have shown that G. boninense has antimicrobial and antagonistic properties. However, there is limited information reported on antifungal properties especially on Candida (C) albicans. Hence, this study was conducted to determine the anti-Candida activity of G. boninense against C albicans. Crude methanolic extracts of G. boninense was obtained by maceration method with 70% methanol. Anti-Candida test was carried out using disc diffusion assay, broth dilution method, time killing profile and brine shrimp toxicity assay. Anti-Candida activity indicated that the mean zone of inhibition was 12.5 +/- 0.6 mm. The MIC value for C. albicans found to be 3.125 mg/ml. The result from time-killing profile showed that the growth of C albicans was inhibited hence decreases its exponential phase. For brine shrimp toxicity assay, the LC50 value was 3.59 mg/ml which proved that the extract of G. boninense is not toxic.

  9. Morphological changes about Artemia Salina by the effect of gamma radiation

    International Nuclear Information System (INIS)

    Dvorak, P.; Kunova, V; Kratochvil, B.; Salplachta, J.; Benova, K.

    2004-01-01

    Direction Council of Europe require restriction of laboratory vertebrae in biological experiments. Once by possibilities is using biological test II. generation among which belongs to and test for Artemia salina. Still this time published test however value only lethality in dependence on dose, exposition and time survival. This work document possibility watch of expressive morphological changes, which consist in retardation development brine shrimps stage in dependence on dose gamma radiation 60 Co. (authors)

  10. Virulence of luminescent and non-luminescent isogenic vibrios towards gnotobiotic Artemia franciscana larvae and specific pathogen-free Litopenaeus vannamei shrimp.

    Science.gov (United States)

    Phuoc, L H; Defoirdt, T; Sorgeloos, P; Bossier, P

    2009-04-01

    This study was conducted to test the virulence of luminescent (L) and non-luminescent (NL) isogenic strains of Vibrio campbellii LMG21363, Vibrio harveyi BB120 (wild type) and quorum-sensing mutant strains derived from the wild type such as Vibrio harveyi BB152, BB170, MM30 and BB886. The NL strains could be obtained by culturing rifampicin-resistant luminescent strains in the dark under static condition. The virulence of the L and NL strains was tested in gnotobiotic Artemia franciscana larvae challenged with 10(4) CFU ml(-1) of bacteria. All luminescent isogenic tested strains showed higher virulence compared to the NL strains. The virulence of L and NL V. campbellii and V. harveyi BB120 was also tested in specific pathogen-free juvenile shrimp upon intramuscular injection with 10(6) CFU of bacteria. In contrast with Artemia, there was no significant difference in mortality between the groups challenged with L and NL strains (P > 0.05). The non-luminescent strains were not able to revert back to the luminescent state and quorum sensing did not influence this phenotypic shift. Luminescent Vibrio strains can switch to a non-luminescent state by culturing them in static conditions. The NL strains become less virulent as verified in Artemia. The luminescent state of Vibrio cells in a culture needs to be verified in order to assure maintenance of virulence.

  11. Effect of brine marination on survival and growth of spoilage and pathogenic bacteria during processing and subsequent storage of ready-to-eat shrimp (Pandalus borealis)

    DEFF Research Database (Denmark)

    Mejlholm, Ole; Devitt, Tina D.; Dalgaard, Paw

    2012-01-01

    The effect of brine marination at chill temperatures on survival and growth of spoilage and pathogenic bacteria during processing and subsequent storage of ready-to-eat cold water shrimp was studied. Survival and growth of Lactobacillus sakei, Listeria monocytogenes, Salmonella, Staphylococcus...... aureus and Vibrio parahaemolyticus were examined. The effect of brine composition and pH was determined in 12 screening experiments without addition of shrimp. Sixteen challenge tests with shrimp were then carried out to examine the effect of brine composition and storage temperature on survival...

  12. Two new coumarins from Micromelum falcatum with cytotoxicity and brine shrimp larvae toxicity.

    Science.gov (United States)

    Luo, Xiongming; He, Weihong; Yin, Hao; Li, Qingxin; Liu, Qiao; Huang, Yongzhong; Zhang, Si

    2012-06-06

    Two new coumarins, 7-methoxy-8-(2-hydroxmethyl-1-O-isovaleryl-4-butenyl)-coumarin (1) and 7-methoxy-8-(1-hydroxy-2-O-β-glucopyranosyl-3-methyl-4-butene-1-yl)coumarin (2), and twelve known coumarins 3-14 were isolated from the stem bark of Micromelum falcatum. The structures of compounds 1-14 were elucidated by extensive spectroscopic data analyses. The toxicity of compounds 1-14 was tested using a brine shrimp assay and in vitro antiproliferative assay against mammary cancer (F10) and lung cancer (HvEvc) cell lines by the MTT method. Some compounds had moderate activities. All compounds were also tested against the microorganisms Bacillus subtilis, Bacillus thuringiensis and Escherichia coli, but no activity was observed.

  13. The genus Artemia Leach, 1819 (Crustacea: Branchiopoda: I. True and false taxonomical descriptions El género Artemia Leach, 1819 (Crustacea: Branchiopoda: I. Descripciones taxonómicas verdaderas y falsas

    Directory of Open Access Journals (Sweden)

    Alireza Asem

    2010-01-01

    Full Text Available The brine shrimp Artemia is important for aquaculture since it is bighly nutritious. It is also used widely in biological studies because it is easy to culture. The aim of the present study is to review the literature on the taxonomical nomenclature of Artemia. The present study indicates the existence of seven species: three living in the Americas, one in Europe, and three in Asia.El camarón de salmuera Artemia es importante para la acuicultura por su alta calidad nutricional y es muy utilizado para estudios biológicos por ser de fácil cultivo. El objetivo del presente estudio es revisar la literatura sobre la nomenclatura taxonómica de Artemia. Se determina la existencia de siete especies; tres de ellas viven en América, una en Europa y tres en Asia.

  14. Phloroglucinol-Mediated Hsp70 Production in Crustaceans: Protection against Vibrio parahaemolyticus in Artemia franciscana and Macrobrachium rosenbergii

    Directory of Open Access Journals (Sweden)

    Vikash Kumar

    2018-05-01

    Full Text Available The halophilic aquatic bacterium, Vibrio parahaemolyticus, is an important aquatic pathogen, also capable of causing acute hepatopancreatic necrosis disease (AHPND in shrimp resulting in significant economic losses. Therefore, there is an urgent need to develop anti-infective strategies to control AHPND. The gnotobiotic Artemia model is used to establish whether a phenolic compound phloroglucinol is effective against the AHPND strain V. parahaemolyticus MO904. We found that pretreatment with phloroglucinol, at an optimum concentration (30 µM, protects axenic brine shrimp larvae against V. parahaemolyticus infection and induced heat shock protein 70 (Hsp70 production (twofolds or more as compared with the control. We further demonstrated that the Vibrio-protective effect of phloroglucinol was caused by its prooxidant effect and is linked to the induction of Hsp70. In addition, RNA interference confirms that phloroglucinol-induced Hsp70 mediates the survival of brine shrimp larvae against V. parahaemolyticus infection. The study was validated in xenic Artemia model and in a Macrobrachium rosenbergii system. Pretreatment of xenic brine shrimp larvae (30 µM and Macrobrachium larvae (5 µM with phloroglucinol increases the survival of xenic brine shrimp and Macrobrachium larvae against subsequent V. parahaemolyticus challenge. Taken together, our study provides substantial evidence that the prooxidant activity of phloroglucinol induces Hsp70 production protecting brine shrimp, A. franciscana, and freshwater shrimp, M. rosenbergii, against the AHPND V. parahaemolyticus strain MO904. Probably, phloroglucinol treatment might become part of a holistic strategy to control AHPND in shrimp.

  15. Estimation of antioxidant, antimicrobial activity and brine shrimp toxicity of plants collected from Oymyakon region of the Republic of Sakha (Yakutia, Russia

    Directory of Open Access Journals (Sweden)

    Babita Paudel

    2014-01-01

    Full Text Available BACKGROUND: Several plants are reported to be produced various biological active compounds. Lichens from the extreme environments such as high altitude, high UV, drought and cold are believed to be synthesized unique types of secondary metabolites than the other one. Several human pathogenic bacteria and fungi have been muted into drug resistant strains. Various synthetic antioxidant compounds have posed carcinogenic effects. This phenomenon needs further research for new effective drugs of natural origin. This manuscript aimed to screen new source of biological active compounds from plants of subarctic origin. RESULTS: A total of 114 plant species, including 80 species of higher plants, 19 species of lichens and 15 species of mosses, were collected from Oymyakon region of the Republic of Sakha (Yakutia, Russia (63˚20′N, 141˚42′E - 63˚15′N, 142˚27′E. Antimicrobial, DPPH free radical scavenging and brine shrimp (Artemia salina toxicity of all crude extract were evaluated. The obtained result was analyzed and compared with commercial standards. A total of 28 species of higher plants showed very strong antioxidant activity (DPPH IC50, 0.45-5.0 µg/mL, 13 species showed strong activity (DPPH IC50, 5-10 µg/mL, 22 species showed moderate antioxidant activity (DPPH IC50,10-20 µg/mL and 17 species showed weak antioxidant activity (DPPH IC50 more than 20 µg/mL. Similarly, 3 species of lichen showed strong antioxidant activity, one species showed moderate and 15 species showed weak DPPH reducing activity. In addition, 4 species of mosses showed moderate antioxidant activity and 11 species showed weak antioxidant activity. Similarly, extracts of 51 species of higher plants showed antimicrobial (AM activity against Staphylococcus aureus and 2 species showed AM activity against Candida albicans. Similarly, 11 species of lichen showed AM activity against S. aureus and 3 species showed AM activity against Escherichia coli. One species of moss

  16. SALT EFFECTS ON EGGS AND NAUPLII OF ARTEMIA SALINA L

    Science.gov (United States)

    Boone, Eleanor; Baas-Becking, L. G. M.

    1931-01-01

    Eggs of Artemia salina L., the brine shrimp, are easily obtainable in large quantities. Ecdysis takes place in two stages: (a) extrusion of the inner membrane, and (b) ecdysis of the nauplius from that membrane. The conditions which allow for the former are much more varied than those for the latter. Nauplii form in only solutions of a few sodium salts; and, in Mg, Ca, and Sr salts, potassium is very toxic. The possible environment for the nauplii (1 M total molarity) has been ascertained for chlorides of Na, K, Mg, and Ca. The facts observed account for the peculiar distribution of the organism. PMID:19872620

  17. Studies on antimicrobial activity and brine shrimp lethality of crude samples of six different species of puffer fishes

    Directory of Open Access Journals (Sweden)

    Masilamani Mohan Raj

    2015-07-01

    Full Text Available Objective: To evaluate the antimicrobial activity and brine shrimp lethality activity of six different species of puffer fishes, including Cyclichthys orbicularis, Diodon holocanthus, Canthigaster solandri, Arthron hispidus, A. inermis and Lagocephalua inermis (L. inermis. Methodology: The puffer fishes were collected from Annangkovil Fish Landing Centre (Lattitude 11°30.47' N; Longitude 79°47.02' E, Parangipettai, Southeast Coast of India during summer season because of availability. Fresh tissue samples were collected from the clearly washed specimens, extracted with methanol at 37 °C for 3 days and filtered through Whatman No. 1 filter paper. The solvents such as methanol and ethanol were concentrated by using rotary evaporator under reduced pressure. The dark brown gummy mass was stored at 4 °C for further analysis. Prepared crude samples were analysed with human pathogens to assess the antibacterial activity and this was carried out by using standard disc diffusion method. The brine shrimp lethality was calculated as the percentage of mortality which was firstly calculated by dividing the number of dead larvae by the total number and then multiplied to 100%. Results: The antibacterial activity of crude extract of puffer fishes were exhibited against 10 different human bacterial pathogens. Among the ten human pathogens, Arthron hispidus showed maximum zone of inhibition (8 mm against Staphylococcus aureus while L. inermis showed minimum activity (1 mm against Proteus mirabilis and no zone of inhibition was observed against Staphylococcus aureus. Brine shrimp lethality was examined with six puffer fish extracts. Cyclichthys orbicularis showed maximum mortalities as 100% and L. inermis showed minimum mortalities as 70% at a concentration of 500 µg/mL. Conclusion: In conclusion, the study showed the preliminary investigation of crude extracts of puffer fishes about the prominent activity against human bacterial pathogens. The extracts had

  18. Acute toxicity, brine shrimp cytotoxicity and relaxant activity of fruits of callistemon citrinus curtis

    Directory of Open Access Journals (Sweden)

    Shah Ismail

    2011-10-01

    Full Text Available Abstract Background Callistemon citrinus Curtis belongs to family Myrtaceae that has a great medicinal importance. In our previous work, fruits of Callistemon citrinus were reported to have relaxant (antispasmodic activity. The current work describes the screening of fractions of the crude methanol extract for tracing spasmolytic constituents so that it shall help us for isolation of bioactive compounds. Acute toxicity and brine shrimp cytotoxicity of crude methanol extract are also performed to standardize it. Methods The crude methanol extract was obtained by maceration with distilled water (500 ml three times and fractionated successively with n-hexane, chloroform, ethyl acetate and n-butanol (300 ml of each solvent. Phytochemical analysis for crude methanol extract was performed. Acute toxicity studies were performed in mice. Brine shrimp cytotoxicity studies were performed to determine its cytotoxicity and standardize it. In other series of experiments, rabbits' jejunum preparations were used in screening for possible relaxant activities of various fractions. They were applied in concentrations of 0.01, 0.03, 0.1, 0.3, 1.0, 3.0, 5.0 and 10.0 mg/ml on spontaneous rabbits' jejunum preparations. In similar fashion, fractions were also tested on KCl (80 mM -induced contractions. Calcium chloride curves were constructed in K-rich Tyrode's solution. The effects of various fractions were tested on calcium chloride curves at concentrations 1.0, 3.0, 5.0 and 10.0 mg/ml. Curves of verapamil used as reference drug at concentration 0.1 μM and 0.3 μM were also constructed. The curves were compared with their respective controls for possible right shift. Results Methanol extract tested strongly positive for saponins and tannins. However, it tested mild positive for presence of proteins, amino acids, carbohydrates and phenolic compounds. LD50 value for crude methanol extract is 476.25 ± 10.3 (470-481, n = 4 mg/ml. Similarly, EC50 value for brine shrimp

  19. Effect of light and brine shrimp on skeletal δ 13C in the Hawaiian coral Porites compressa: a tank experiment

    Science.gov (United States)

    Grottoli, Andréa G.

    2002-06-01

    Previous experimental fieldwork showed that coral skeletal δ13C values decreased when solar intensity was reduced, and increased in the absence of zooplankton. However, actual seasonal changes in solar irradiance levels are typically less pronounced than those used in the previous experiment and the effect of increases in the consumption of zooplankton in the coral diet on skeletal δ13C remains relatively unknown. In the present study, the effects of four different light and heterotrophy regimes on coral skeletal δ13C values were measured. Porites compressa corals were grown in outdoor flow-through tanks under 112%, 100%, 75%, and 50% light conditions at the Hawaii Institute of Marine Biology, Hawaii. In addition, corals were fed either zero, low, medium, or high concentrations of brine shrimp. Decreases in light from 100% resulted in significant decreases in δ13C that is most likely due to a corresponding decrease in photosynthesis. Increases in light to 112% also resulted in a decrease in δ13C values. This latter response may be a consequence of photoinhibition. The overall curved response in δ13C values was described by a significant quadratic function. Increases in brine shrimp concentrations resulted in increased skeletal δ13C levels. This unexpected outcome appears to be attributable to enhanced nitrogen supply associated with the brine shrimp diet which led to increased zooxanthellae concentrations, increased photosynthesis rates, and thus increased δ13C values. This result highlights the potential influence of nutrients from heterotrophically acquired carbon in maintaining the zooxanthellae-host symbiosis in balance. In addition, evidence is presented that suggests that coral skeletal growth and δ13C are decoupled. These results increase our knowledge of how light and heterotrophy affects the δ13C of coral skeletons.

  20. Larvicidal, antimicrobial and brine shrimp activities of extracts from Cissampelos mucronata and Tephrosia villosa from coast region, Tanzania.

    Science.gov (United States)

    Nondo, Ramadhani S O; Mbwambo, Zakaria H; Kidukuli, Abdul W; Innocent, Ester M; Mihale, Matobola J; Erasto, Paul; Moshi, Mainen J

    2011-04-23

    The leaves and roots of Cissampelos mucronata A. Rich (Menispermaceae) are widely used in the tropics and subtropics to manage various ailments such as gastro-intestinal complaints, menstrual problems, venereal diseases and malaria. In the Coast region, Tanzania, roots are used to treat wounds due to extraction of jigger. Leaves of Tephrosia villosa (L) Pers (Leguminosae) are reported to be used in the treatment of diabetes mellitus in India. In this study, extracts from the roots and aerial parts of C. mucronata and extracts from leaves, fruits, twigs and roots of T. villosa were evaluated for larvicidal activity, brine shrimps toxicity and antimicrobial activity. Powdered materials from C. mucronata were extracted sequentially by dichloromethane followed by ethanol while materials from T.villosa were extracted by ethanol only. The extracts obtained were evaluated for larvicidal activity using Culex quinquefasciatus Say larvae, cytotoxicity using brine shrimp larvae and antimicrobial activity using bacteria and fungi. Extracts from aerial parts of C. Mucronata exhibited antibacterial activity against Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Salmonella typhi, Vibrio cholera, Bacillus anthracis, Streptococcus faecalis and antifungal activity against Candida albicans and Cryptococcus neoformans. They exhibited very low toxicity to brine shrimps and had no larvicidal activity. The root extracts exhibited good larvicidal activity but weak antimicrobial activity. The root dichloromethane extracts from C. mucronata was found to be more toxic with an LC50 value of 59.608 μg/mL while ethanolic extracts from root were not toxic with LC50>100 μg/mL). Ethanol extracts from fruits and roots of T. villosa were found to be very toxic with LC50 values of 9.690 μg/mL and 4.511 μg/mL, respectively, while, ethanol extracts from leaves and twigs of T. villosa were found to be non toxic (LC50>100 μg/mL). These results support the use of C. mucronata in

  1. Larvicidal, antimicrobial and brine shrimp activities of extracts from Cissampelos mucronata and Tephrosia villosa from coast region, Tanzania

    Directory of Open Access Journals (Sweden)

    Erasto Paul

    2011-04-01

    Full Text Available Abstract Background The leaves and roots of Cissampelos mucronata A. Rich (Menispermaceae are widely used in the tropics and subtropics to manage various ailments such as gastro-intestinal complaints, menstrual problems, venereal diseases and malaria. In the Coast region, Tanzania, roots are used to treat wounds due to extraction of jigger. Leaves of Tephrosia villosa (L Pers (Leguminosae are reported to be used in the treatment of diabetes mellitus in India. In this study, extracts from the roots and aerial parts of C. mucronata and extracts from leaves, fruits, twigs and roots of T. villosa were evaluated for larvicidal activity, brine shrimps toxicity and antimicrobial activity. Methods Powdered materials from C. mucronata were extracted sequentially by dichloromethane followed by ethanol while materials from T.villosa were extracted by ethanol only. The extracts obtained were evaluated for larvicidal activity using Culex quinquefasciatus Say larvae, cytotoxicity using brine shrimp larvae and antimicrobial activity using bacteria and fungi. Results Extracts from aerial parts of C. Mucronata exhibited antibacterial activity against Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Salmonella typhi, Vibrio cholera, Bacillus anthracis, Streptococcus faecalis and antifungal activity against Candida albicans and Cryptococcus neoformans. They exhibited very low toxicity to brine shrimps and had no larvicidal activity. The root extracts exhibited good larvicidal activity but weak antimicrobial activity. The root dichloromethane extracts from C. mucronata was found to be more toxic with an LC50 value of 59.608 μg/mL while ethanolic extracts from root were not toxic with LC50>100 μg/mL. Ethanol extracts from fruits and roots of T. villosa were found to be very toxic with LC50 values of 9.690 μg/mL and 4.511 μg/mL, respectively, while, ethanol extracts from leaves and twigs of T. villosa were found to be non toxic (LC50>100

  2. Effect of concentrations of Artemia salina on zootechnical ...

    African Journals Online (AJOL)

    The best performances of survival, growth and metamorphosis were produced by 3 AN/ml concentrations of Artemia. The cost of food has increased with the concentration level of Artemia. Then, the production of viable shrimp post larvae and cheaper may be done at 3 AN/ml optimal concentrations of Artemia. Keywords: ...

  3. Identification of lactic acid bacteria from spoilage associations of cooked and brined shrimps stored under modified atmosphere between 0 degrees C and 25 degrees C

    DEFF Research Database (Denmark)

    Dalgaard, Paw; Vancanneyt, M.; Vilalta, N.E.

    2003-01-01

    MAP shrimps were characterized by phenotypic tests and identified as lactic acid bacteria (78 isolates), other Gram-positive bacteria (13 isolates) and Gram-negative bacteria (11 isolates). A selection of 48 LAB isolates were further characterized and identified by phenotypic tests and SDS-PAGE...... the dominant parts of spoilage associations of cooked and brined MAP shrimps stored at high and low temperatures, respectively. Significance and Impact of the Study: The SDS-PAGE technique and simple biochemical keys allowed the majority of LAB isolates from spoilage associations of cooked and brined MAP...

  4. Adult Artemia as food for juvenile prawns

    Digital Repository Service at National Institute of Oceanography (India)

    Royan, J.P.; Sumitra-Vijayaraghavan; Krishnakumari, L.

    Feeding experiments on three commercially important shrimps Penaues indicus, Metapenaeus dobsoni and M. monoceros were carried out using adult Artemia as feed. Good growth and food conversion efficiencies were recorded at 15% feeding level in all...

  5. Influence of Chemical Parameters on Artemia sp. (Crustacea: Anostraca Population in Al Wathba Lake in the Abu Dhabi Emirate, UAE

    Directory of Open Access Journals (Sweden)

    Anitha Saji

    2016-04-01

    Full Text Available Long term monitoring programme on Brine shrimp (Artemia sp. is being carried out by the Environment Agency, Abu Dhabi, United Arab Emirates (EAD with the prime purpose of understanding the population dynamics, ecology and habitat requirements of Artemia at Al Wathba Lake, situated within Al Wathba Wetland Reserve, which is an artificial wetland near Abu Dhabi City. The present study, being a component of this programme, intends to understand the influence of chemical parameters such as dissolved oxygen, nitrate, nitrite, phosphate, ammonia and total organic carbon on Artemia biomass and cyst production at different sites of the Al Wathba Lake. The study was carried out by sampling lake water quarterly for a period of 5 years from 2010 to 2014. The Artemia population was found to have direct impact of the above mentioned parameters on its abundance. The abundance was highest during the year 2010. Further, temperature, dissolved oxygen, nitrate and cadmium were found to be the most crucial parameters for production of Artemia. The study further aimed to determine the significant relationship between physico-chemical parameters and Artemia sp. population dynamics and cyst production.

  6. Toxicity and Binding Profile of Lectins from the Genus Canavalia on Brine Shrimp

    Directory of Open Access Journals (Sweden)

    Francisco Vassiliepe Sousa Arruda

    2013-01-01

    Full Text Available Lectins are sugar-binding proteins widely distributed in nature with many biological functions. Although many lectins have a remarkable biotechnological potential, some of them can be cytotoxic. Thus, the aim of this study was to assess the toxicity of five lectins, purified from seeds of different species of Canavalia genus. In order to determine the toxicity, assays with Artemia nauplii were performed. In addition, a fluorescence assay was carried out to evaluate the binding of lectins to Artemia nauplii. In order to verify the relationship between the structure of lectins and their cytotoxic effect, structural analysis was carried out to evaluate the volume of the carbohydrate recognition domain (CRD of each lectin. The results showed that all lectins exhibited different toxicities and bound to a similar area in the digestive tract of Artemia nauplii. Concerning the structural analysis, differences in spatial arrangement and volume of CRD may explain the variation of the toxicity exhibited by each lectin. To this date, this is the first study that establishes a link between toxicity and structure of CRD from Diocleinae lectins.

  7. Bioactivity evaluation against Artemia salina Leach of medicinal plants used in Brazilian Northeastern folk medicine.

    Science.gov (United States)

    Arcanjo, D D R; Albuquerque, A C M; Melo-Neto, B; Santana, L C L R; Medeiros, M G F; Citó, Amgl

    2012-08-01

    The brine shrimp (Artemia salina Leach) lethality bioassay offers an advantage in standardization and quality control of botanical products. This test is well correlated with antitumor activity (cytotoxicity) and can be used to monitor the activity of bioactive natural products. This paper reports the bioactivity of ethanol extracts from seven medicinal plants from the Northeast of Brazil (Acmella uliginosa, Ageratum conyzoides, Eugenia uniflora, Plectranthus neochilus, Moringa oleifera, Justicia pectoralis and Equisetum sp.) against Artemia salina. Biological activity was evaluated for extracts at 1, 10, 100, and 1000 µg/mL in triplicate, and the mean lethal concentration values (LC50) were obtained by probit analysis. The species Acmella uliginosa showed the highest bioactivity, and its flower extract was more active than its leaf extract.

  8. Investigations of the effects of cosmic rays on Artemia cysts and tobacco seeds: results of Exobloc II experiment, flown aboard Biocosmos 1887

    Energy Technology Data Exchange (ETDEWEB)

    Gaubin, Y; Pianezzi, B; Gasset, G; Planel, H [Laboratoire de Biologie Medicale, Faculte de Medecine, Toulouse (France); Delpoux, M [Laboratoire de Biogeographie, Faculte des Sciences, Toulouse (France); Heilmann, C [Strasbourg-1 Univ., 67 (France). Centre de Recherches Nucleaires

    1990-01-01

    Artemia (Brine shrimp) cysts and tobacco seeds, dormant biological material devoid of metabolic activity, were flown aboard the Soviet Biocosmos 1887 in order to investigate the effects of cosmic rays. Artemia cysts and tobacco seeds were used in bulk or in monolayers sandwiched with track detectors. Biological and physical units were located outside and inside the spacecraft. Stacks included lead shielding in order to expose the objects to different doses of radiation. Total dosimetry was performed using thermoluminescent detectors. In spite of low levels of doses, the space flight resulted in a decrease in developmental capacity of Artemia cysts, and in a higher mutation rate in tobacco seeds. The more obvious responses occurred, in both cases, in biological objects exposed to the highest doses. These results are compared to those of previous space experiments. (author).

  9. Parasiticidal and brine shrimp cytotoxicity potential of crude methanolic extract of rind of Punica granatum Linn against round worms and tape worms.

    Science.gov (United States)

    Ali, Niaz; Jamil, Ayesha; Shah, Syed Wadood Ali; Shah, Ismail; Ahmed, Ghayour; Junaid, Muhammad; Ahmed, Zahoor

    2015-05-01

    Rind of Punica granatum is traditionally used for anthelmintic purposes. The current work describes the possible anthelmintic activity of crude methanolic extract of Punica granatum (Pg. Cr) against round worms (Ascaridia galli) and the tape worms (Raillietina spiralis). Brine shrimp cytotoxicity is also performed. Brine shrimp cytotoxic activity was tested using different concentrations (1000 μg/mL, 100 μg/mL and 10 μg/mL) of Pg.Cr. In vitro anthelmintic activity of Pg. Cr was determined against the parasites using albendazole and piperazine citrate as standard anthelmintic drugs in concentration 10 mg/ml. LC50 value for Brine shrimp cytotoxicity was 189.44 ±28 μg/mL. In test concentration of 40mg/ml of the Pg. Cr, Raillietina spiralis was paralyzed in 23 minutes. However, for parasiticidal activity (death of the parasite), it took less time (40 minutes) as compared to standard Albendazole. Time taken for death of the parasite Raillietina spiralis, in concentration 40 mg /ml, is 40 min. While standard drugs took more time to kill the Raillietina spiralis. Pg. Cr took 19 minutes to paralyze the Ascaridia galli at concentration 40 mg/ml whereas; it took 48 minutes for to kill the parasite Ascaridia galli. The current work confirms the traditional use of rind of Punica granatum as anthelmintic against Raillietina spiralis and Ascaridia galli. Results of brine shrimp cytotoxicity assay warrant for the isolation of cytotoxic compounds. List of abbreviation- Pg. Cr = Crude methanolic extract of Punica granatum.

  10. Kajian pendahuluan uji toksisitas ekstrak air miselia dan tubuh buah jamur shitake (Lentinus edodes) dengan metode brine shrimp lethality test (BTS)

    OpenAIRE

    Noor Erma NS; Tri Sundari; Arie Ika Susanty; Dwi Riani Octavia Palupi; Isnaeni Isnaeni; Sukardiman Sukardiman

    2012-01-01

    Shiitake mushroom (Lentinus edodes) is one of the wood mushroom types that can be consumed as a food as well as for a medicalpurpose. Lentinan, a polysaccharide contained in shiitake, is well known for its use on cancer medication. Mycelium of Shiitake mushroomcontains lentinan the same as other part of the mushroom like fruity body. Toxicity of the lentinan in mycelium compare to the fruitybody has been first conducted by using Brine Shrimp Lethality Test (BST). Using Potato Dextrose Broth m...

  11. Cytotoxicity of Dorema auchri, Achillea millefolium and Artemisia aucheri by Artemia

    Directory of Open Access Journals (Sweden)

    M Ghavamizadeh

    2013-09-01

    Full Text Available Abstract Background & aim: Nowadays, toxic compounds derived from plants used against microbes and cancer cells. The aim of this study was to evaluate cytotoxicity of Dorema aucheri, Achillea millefollium and Artemisia aucheri using brine shrimp, Artemia urmiana, lethality test. Methods: In this experimental study, the plants were collected from the Kohgiluyeh and Boyer-Ahmad Province, then identified by a botanist. Clean and air-dried aerial parts of plants were extracted with suitable solvents. Cytotoxicity evaluation was performed using larvae hatched cysts were purchased from Urmia. Live larvae were exposed to different concentrations of extract and the numbers of live and dead larvae were counted after 24 hours. Mean of LC 50 of any extracts from control and exposed live larvae were examined. The data were analyzed using the Finney’s Probit analysis. Results: Hydro ethanol and chloroform extracts of Achillea millefolium, Dorema aucheri and Artemisia aucheri exhibited potent brine shrimp lethality with LC50 67.8±0.53μg.ml-1, 76.50±0.60μg.ml-1, 92.70±6.05μg.ml-1 respectively. The degree of lethality was found to be directly proportional to the concentration of extracts. Conclusion: According to BSLT, LC50 of D. aucheri, A. millefolium and A. aucheri, they were considered as toxic. So these plants could be a source of new compounds with biological activity. Key words: Artemia urmiana, Dorema auchri, Achillea millefolium, Artemisia aucheri, Cytotoxicity

  12. Preliminary results of the Artemia salina experiments in biostack on LDEF

    International Nuclear Information System (INIS)

    Graul, E.H.; Ruether, W.; Hiendl, C.O.

    1992-01-01

    The mosaic egg of the brine shrimp, Artemia salina, resting in blastula or gastrula state represents a system that during further development, proceeds without any further development to the larval stage, the free swimming nauplius. Therefore, injury to a single cell of the egg will be manifest in the larvae. In several experiments, it was shown that the passage of a single heavy ion through the shrimp egg damaged a cellular area large enough to disturb either embryogenesis or further development of the larvae, or the integrity of the adult individual. Emergence from the egg shell was heavily disturbed by the heavy ions as was hatching. Additional late effects, due to a hit by a heavy ion, are delayed of growth and of sexual maturity, and reduced fertility. Anomalies in the body and the extremities could be observed more frequently for the nauplii which had developed from eggs hit by heavy ions

  13. Expression and Quorum Sensing Regulation of Type III Secretion System Genes of Vibrio harveyi during Infection of Gnotobiotic Brine Shrimp.

    Directory of Open Access Journals (Sweden)

    H A Darshanee Ruwandeepika

    Full Text Available Type III secretion systems enable pathogens to inject their virulence factors directly into the cytoplasm of the host cells. The type III secretion system of Vibrio harveyi, a major pathogen of aquatic organisms and a model species in quorum sensing studies, is repressed by the quorum sensing master regulator LuxR. In this study, we found that during infection of gnotobiotic brine shrimp larvae, the expression levels of three type III secretion operons in V. harveyi increased within the first 12h after challenge and decreased again thereafter. The in vivo expression levels were highest in a mutant with a quorum sensing system that is locked in low cell density configuration (minimal LuxR levels and lowest in a mutant with a quorum sensing system that is locked in the high cell density configuration (maximal LuxR levels, which is consistent with repression of type III secretion by LuxR. Remarkably, in vivo expression levels of the type III secretion system genes were much (> 1000 fold higher than the in vitro expression levels, indicating that (currently unknown host factors significantly induce the type III secretion system. Given the fact that type III secretion is energy-consuming, repression by the quorum sensing master regulators might be a mechanism to save energy under conditions where it does not provide an advantage to the cells.

  14. Bioassay Screening of the Essential Oil and Various Extracts of Nigella sativa L. Seeds Using Brine Shrimp Toxicity Assay

    Directory of Open Access Journals (Sweden)

    Fariba Sharififar

    2017-03-01

    Full Text Available Background and Aim: Since cytotoxicity screening is the first step necessary for any new drug development, this study was designed to find out and compare the cytotoxicity effects of the essential oil and various extracts of Nigella sativa L. seeds using Brine Shrimp Lethality (BSL assay. Materials and Methods: Essential oils and various extracts of N. sativa were assessed by two methods of disk and solution of BSL. Data analysis was carried out using SPSS statistical package version 17.0 (SPSS Inc., Chicago, IL, 250 USA. Data were processed in probit-analysis program to estimate LC50 values. Results: All of the tested fractions demonstrated more cytotoxicity in the solution method. Petroleum ether and chloroform extract of N. sativa showed the most cytotoxicity with LC50 values 7 and 21 μg/ml respectively; while aqueous and ethanolic had no significant cytotoxicity. Moreover, the GC/MS analysis of the essential oil of N. sativa showed the p-cymene (48.1%, α-thujone (14.38% and dihydro carveol (9.11% as the main compounds. Conclusion: These results suggest some limitation for using this spice in diet. Furthermore, this plant could be considered as a source of cytotoxic compounds which should be studied in details.

  15. Anticipation of Artemia sp. supply in the larviculture of the barber goby Elacatinus figaro (Gobiidae: Teleostei influenced growth, metamorphosis and alkaline protease activity

    Directory of Open Access Journals (Sweden)

    Maria Fernanda da Silva-Souza

    2015-09-01

    Full Text Available The barber goby Elacatinus figaro is considered endangered due to overexploitation by the ornamental industry. Farming marine ornamental fishes, especially the threatened ones, can be one of the measures to minimize the pressure on the natural stocks. Among the priority issues for their production is the determination of the most appropriate feeding management. The feeding protocol commonly used in the larviculture of barber goby, when the start of Artemia sp. offer occurred at the 18th DAH (days after hatching (treatment T18, was modified, by anticipating brine shrimp supply in 6 days (treatment T12. Alkaline proteases activity, growth and metamorphosis of larvae were evaluated in both protocols. Juveniles at T12 showed higher weight (0.04 ± 0.001 g and lower activity of total alkaline proteases (1.3 ± 0.2 mU mg-1 protein compared to T18 (0.02 ± 0.001 g; 2.8 ± 0.4 mU mg-1 protein, respectively. With anticipation of brine shrimp, the commencing and end of larval transformation was observed earlier (at 24 and 34 DAH, respectively in comparison to those with the supply of Artemia sp. at 18 DAH (27 and 41 DAH, respectively. Thus, the Artemia sp. anticipation was beneficial during the larviculture of the barber goby, considering that larvae reached metamorphosis earlier.

  16. High prevalence of cestodes in Artemia spp. throughout the annual cycle: relationship with abundance of avian final hosts

    Science.gov (United States)

    Sánchez, Marta I.; Nikolov, Pavel N.; GEorgieva, Darina D.; Georgiev, Boyko B.; Vasileva, Gergana P.; Pankov, Plamen; Paracuellos, Mariano; Lafferty, Kevin D.; Green, Andy J.

    2013-01-01

    Brine shrimp, Artemia spp., act as intermediate hosts for a range of cestode species that use waterbirds as their final hosts. These parasites can have marked influences on shrimp behavior and fecundity, generating the potential for cascading effects in hypersaline food webs. We present the first comprehensive study of the temporal dynamics of cestode parasites in natural populations of brine shrimp throughout the annual cycle. Over a 12-month period, clonal Artemia parthenogenetica were sampled in the Odiel marshes in Huelva, and the sexual Artemia salina was sampled in the Salinas de Cerrillos in Almería. Throughout the year, 4–45 % of A. parthenogenetica were infected with cestodes (mean species richness = 0.26), compared to 27–72 % of A. salina (mean species richness = 0.64). Ten cestode species were recorded. Male and female A. salina showed similar levels of parasitism. The most prevalent and abundant cestodes were those infecting the most abundant final hosts, especially the Greater Flamingo Phoenicopterus ruber. In particular, the flamingo parasite Flamingolepis liguloides had a prevalence of up to 43 % in A. parthenogenetica and 63.5 % in A. salina in a given month. Although there was strong seasonal variation in prevalence, abundance, and intensity of cestode infections, seasonal changes in bird counts were weak predictors of the dynamics of cestode infections. However, infection levels of Confluaria podicipina in A. parthenogenetica were positively correlated with the number of their black-necked grebe Podiceps nigricollis hosts. Similarly, infection levels of Anomotaenia tringae and Anomotaenia microphallos in A. salina were correlated with the number of shorebird hosts present the month before. Correlated seasonal transmission structured the cestode community, leading to more multiple infections than expected by chance.

  17. Study of inter species diversity and population structure by molecular genetic method in Iranian Artemia

    OpenAIRE

    Hajirostamloo, Mahbobeh

    2005-01-01

    Artemia is a small crustacean that adapted to live in brine water and has been seen in different brine water sources in Iran. Considering the importance of genetic studies manifest inter population differences in species, to estimate genetic structure, detect difference at molecular level and separate different Artemia populations of Iran, also study of phylogenic relationships among them, samples of Artemia were collected from nine region: Urmia lake in West Azerbaijan, Sh...

  18. Acute toxicity, bioaccumulation and effects of dietary transfer of silver from brine shrimp exposed to PVP/PEI-coated silver nanoparticles to zebrafish.

    Science.gov (United States)

    Lacave, José María; Fanjul, Álvaro; Bilbao, Eider; Gutierrez, Nerea; Barrio, Irantzu; Arostegui, Inmaculada; Cajaraville, Miren P; Orbea, Amaia

    2017-09-01

    The extensive use and release to the aquatic environment of silver nanoparticles (NPs) could lead to their incorporation into the food web. Brine shrimp larvae of 24h showed low sensitivity to the exposure to PVP/PEI-coated Ag NPs (5nm), with EC 50 values at 24h of 19.63mgAgL -1 , but they significantly accumulated silver after 24h of exposure to 100μgL -1 of Ag NPs. Thus, to assess bioaccumulation and effects of silver transferred by the diet in zebrafish, brine shrimp larvae were exposed to 100ngL -1 of Ag NPs as an environmentally relevant concentration or to 100μgL -1 as a potentially effective concentration and used to feed zebrafish for 21days. Autometallography revealed a dose- and time-dependent metal accumulation in the intestine and in the liver of zebrafish. Three-day feeding with brine shrimps exposed to 100ngL -1 of Ag NPs was enough to impair fish health as reflected by the significant reduction of lysosomal membrane stability and the presence of vacuolization and necrosis in the liver. However, dietary exposure to 100μgL -1 of Ag NPs for 3days did not significantly alter gene transcription levels, neither in the liver nor in the intestine. After 21days, biological processes such as lipid transport and localization, cellular response to chemical stimulus and response to xenobiotic stimulus were significantly altered in the liver. Overall, these results indicate an effective dietary transfer of silver and point out to liver as the main target organ for Ag NP toxicity in zebrafish after dietary exposure. Copyright © 2017 Elsevier Inc. All rights reserved.

  19. Factors influencing Artemia populations in Antillean salines

    NARCIS (Netherlands)

    Kristensen, Ingvar; Hulscher-Emeis, T. Marian

    1972-01-01

    In the Netherlands Antilles two extreme “forms” of Artemia salina, f. arietina and f. milhauseni, show all intermediate gradations. They hybridize constantly. When forma arietina is moved from lower concentrations into highly concentrated brine it will produce offspring that exhibit characteristics

  20. UJI TOKSISITAS (Brine Shrimp Lethality Test DAN AKTIVITAS ANTIOKSIDAN DARI DAUN SINTRONG (Crassocephalum Crepidioides DENGAN METODE 2,2-diphenyl-1-picrylhidrazil (DPPH

    Directory of Open Access Journals (Sweden)

    Fiktor Boni Pasilala

    2016-11-01

    Full Text Available The phytochemical test, brine shrimp lethality test and antioxidant activity test on secondary metabolits of terap (Artocarpus odoratissimus B. leaves has been completed. The leaves samples are extracted by masseration method that is concentrated by using rotary evaporator. The total extract are fractioned with n-hexane and ethyl acetate. Based on secondary metabolits phytochemical test of terap (Artocarpus odoratissimus B. leaves showed that total extract and Ethyl Acetat fraction extract contains alkaloids, phenolics, flavonoids, saponins, steroids and triterpenoids. N-hexane fraction extract contain alkaloids, steroids and tritepenoids. In brine shrimp lethality test, the increase larvae death data was recorded and processed using SAS Probit Analysis to determine the Lethal Concentration 50 % (LC50 value. The results of this test showed that the most active extract is metanol extract with LC50 value of 88.0227 ppm. Based on the antioxidant activity by scavenging activity of DPPH used spectrophotometry was obtained that Inhibition Concentration 50% (IC50 of total extract is 369.0833 ppm, extract of n-hexane fraction is 1532.267 ppm and extract of ethyl acetat fraction is 82.89003 ppm.

  1. Molecularly characterized solvent extracts and saponins from Polygonum hydropiper L show high anti-angiogenic, anti-tumor, brine shrimp and fibroblast NIH/3T3 cell line cytotoxicity

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    Muhammad eAyaz

    2016-03-01

    Full Text Available Polygonum hydropiper is used as anti-cancer and anti-rheumatic agent in folk medicine. This study was designed to investigate the anti-angiogenic, anti-tumor and cytotoxic potentials of different solvent extracts and isolated saponins. Samples were analyzed using GC, GC-MS to identify major and bioactive compounds. Quantitation of antiangiogenesis for the plant's samples including methanolic extract (Ph.Cr, its subsequent fractions; n-hexane (Ph.Hex, chloroform (Ph.Chf, ethyl acetate (Ph.EtAc, n-Butanol (Ph.Bt, aqueous (Ph.Aq, saponins (Ph.Sp were performed using the chick embryo chorioallantoic membrane (CAM assay. Potato disc anti-tumor assay was performed on Agrobacterium tumefaciens containing tumor inducing plasmid. Cytotoxicity was performed on Artemia salina and mouse embryonic fibroblast NIH/3T3 cell line using brine shrimps and MTT cells viability assays. The GC-MS analysis of Ph.Cr, Ph.Hex, Ph.Chf, Ph.Bt and Ph.EtAc identified 126, 124, 153, 131 and 164 compounds respectively. In anti-angiogenic assay, Ph.Chf, Ph.Sp, Ph.EtAc and Ph.Cr exhibited highest activity with IC50 of 28.65, 19.21, 88.75 and 461.53 µg/ml respectively. In anti-tumor assay, Ph.Sp, Ph.Chf, Ph.EtAc and Ph.Cr were most potent with IC50 of 18.39, 73.81, 217.19 and 342.53 µg/ml respectively. In MTT cells viability assay, Ph.Chf, Ph.EtAc, Ph.Sp were most active causing 79.00, 72.50 and 71.50% cytotoxicity respectively at 1000 µg/ml with the LD50 of 140, 160 and 175 µg/ml respectively. In overall study, Ph.Chf and Ph.Sp have shown overwhelming results which signifies their potentials as sources of therapeutic agents against cancer.

  2. Swimming speed alteration of Artemia sp. and Brachionus plicatilis as a sub-lethal behavioural end-point for ecotoxicological surveys.

    Science.gov (United States)

    Garaventa, Francesca; Gambardella, Chiara; Di Fino, Alessio; Pittore, Massimiliano; Faimali, Marco

    2010-03-01

    In this study, we investigated the possibility to improve a new behavioural bioassay (Swimming Speed Alteration test-SSA test) using larvae of marine cyst-forming organisms: e.g. the brine shrimp Artemia sp. and the rotifer Brachionus plicatilis. Swimming speed was investigated as a behavioural end-point for application in ecotoxicology studies. A first experiment to analyse the linear swimming speed of the two organisms was performed to verify the applicability of the video-camera tracking system, here referred to as Swimming Behavioural Recorder (SBR). A second experiment was performed, exposing organisms to different toxic compounds (zinc pyrithione, Macrotrol MT-200, and Eserine). Swimming speed alteration was analyzed together with mortality. The results of the first experiment indicate that SBR is a suitable tool to detect linear swimming speed of the two organisms, since the values have been obtained in accordance with other studies using the same organisms (3.05 mm s(-1) for Artemia sp. and 0.62 mm s(-1) for B. plicatilis). Toxicity test results clearly indicate that swimming speed of Artemia sp. and B. plicatilis is a valid behavioural end-point to detect stress at sub-lethal toxic substance concentrations. Indeed, alterations in swimming speed have been detected at toxic compound concentrations as low as less then 0.1-5% of their LC(50) values. In conclusion, the SSA test with B. plicatilis and Artemia sp. can be a good behavioural integrated output for application in marine ecotoxicology and environmental monitoring programs.

  3. IDENTIFIKASI DAN UJI AKTIVITAS ANTIKANKER EKSTRAK SPONS Ianthella basta TERHADAP LARVA Artemia salina L.

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    Ni Wayan Sri Sukmarianti

    2013-05-01

    Full Text Available The main purpose of this research is to determine the toxicity of extracts sponge Ianthella basta against Artemia salina larvae and to identify the chemical compounds contained in those toxic isolates. The preliminary test of the anticancer activity has conducted by Brine Shrimp Letalithy (BST test. The results showed that the chloroform extract was the most toxic with LC50 value of 22,39 ppm. Futher, the cloroform extract was separated and purified by coloumn chromatography using eluent of solvent mixture of chloroform : ethyl acetate : n-hexane by 7 : 2 : 1 and 4 fractions were obtained. The most toxic fraction was the fraction C with LC50 value of 35,36 ppm. Based on the GC-MS results, the toxic isolate is allegedly containing chemicals compound of hexadecanoic methyl ester and hexadecanoic acid.

  4. Interference from ordinarily used solvents in the outcomes of Artemia salina lethality test

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    Sahgal Geethaa

    2013-01-01

    Full Text Available Methanol, ethanol, Tween 20 and dimethyl sulfoxide (DMSO are widely used as dissolving agents in Artemia salina lethality test (aka brine shrimp lethality test [BSLT] to screen the pharmaceutical properties of natural products. Nevertheless, there is lack of toxicity level of these solvents against brine shrimp. High concentration of these organic solvent might be toxic for this zoology invertebrate and interfere in the experimental outcomes. To avoid this, permissible concentration of the solvents used in BSLT was identified. BSLT was performed to evaluate the toxicity effect of Tween 20, methanol, ethanol and DMSO at 24 h post-treatment time point against A. salina. The suggested maximum working concentration (v/v for DMSO, methanol, ethanol was found to be 1.25% and that for Tween 20 was 0.16%. LC 50 for the solvents were 8.5% (DMSO, 6.4% (methanol, 3.4% (ethanol and 2.5% (Tween 20. The findings have shown a toxicity level among the solvents in descending order as Tween 20 > ethanol > methanol > DMSO. DMSO is a safer solvent to be used in BSLT compared with other tested solvents, whereas Tween 20 has been shown to be the most stringent solvent among the tested solvents. The findings are resourcefully useful to avoid interference of solvents in the assessment of natural products using BSLT.

  5. Kajian pendahuluan uji toksisitas ekstrak air miselia dan tubuh buah jamur shitake (Lentinus edodes dengan metode brine shrimp lethality test (BTS

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    Noor Erma NS

    2012-02-01

    Full Text Available Shiitake mushroom (Lentinus edodes is one of the wood mushroom types that can be consumed as a food as well as for a medicalpurpose. Lentinan, a polysaccharide contained in shiitake, is well known for its use on cancer medication. Mycelium of Shiitake mushroomcontains lentinan the same as other part of the mushroom like fruity body. Toxicity of the lentinan in mycelium compare to the fruitybody has been first conducted by using Brine Shrimp Lethality Test (BST. Using Potato Dextrose Broth media with the growth rate of3.88% did mycelium multiplications. Probit analysis showed that the toxicity of the mushroom’s cap, stem, and mycelium of Shiitakemushrooms is LC50 = 648.76507 mg/ml LC50 = 489.39444 mg/ml, and LC50 = 481.16941 mg/ml respectively.

  6. Multiple γ-glutamylation: A novel type of post-translational modification in a diapausing Artemia cyst protein

    International Nuclear Information System (INIS)

    Hasegawa, Mai; Ikeda, Yuka; Kanzawa, Hideaki; Sakamoto, Mika; Goto, Mina; Tsunasawa, Susumu; Uchiumi, Toshio; Odani, Shoji

    2010-01-01

    A highly hydrophilic, glutamate-rich protein was identified in the aqueous phenol extract from the cytosolic fraction of brine shrimp (Artemia franciscana) diapausing cysts and termed Artemia phenol soluble protein (PSP). Mass spectrometric analysis revealed the presence of many protein peaks around m/z 11,000, separated by 129 atomic mass units; this value corresponds to that of glutamate, which is strongly suggestive of heterogeneous polyglutamylation. Polyglutamylation has long been known as the functionally important post-translational modification of tubulins, which carry poly(L-glutamic acid) chains of heterogeneous length branching off from the main chain at the γ-carboxy groups of a few specific glutamate residues. In Artemia PSP, however, Edman degradation of enzymatic peptides revealed that at least 13, and presumably 16, glutamate residues were modified by the attachment of a single L-glutamate, representing a hitherto undescribed type of post-translational modification: namely, multiple γ-glutamylation or the addition of a large number of glutamate residues along the polypeptide chain. Although biological significance of PSP and its modification is yet to be established, suppression of in vitro thermal aggregation of lactate dehydrogenase by glutamylated PSP was observed.

  7. Determination of extremely high pressure tolerance of brine shrimp larvae by using a new pressure chamber system.

    Science.gov (United States)

    Seo, Mihye; Koyama, Sumihiro; Toyofuku, Takashi; Kojima, Shigeaki; Watanabe, Hiromi

    2013-11-01

    Hydrostatic pressure is the only one of a range of environmental parameters (water temperature, salinity, light availability, and so on) that increases in proportion with depth. Pressure tolerance is therefore essential to understand the foundation of populations and current diversity of faunal compositions at various depths. In the present study, we used a newly developed pressure chamber system to examine changes in larval activity of the salt-lake crustacean, Artemia franciscana, in response to a range of hydrostatic pressures. We showed that A. franciscana larvae were able to survive for a short period at pressures of ≤ 60 MPa (approximately equal to the pressure of 6000 m deep). At a pressure of > 20 MPa, larval motor ability was suppressed, but not lost. Meanwhile, at a pressure of > 40 MPa, some of the larval motor ability was lost without recovery after decompression. For all experiments, discordance of movement and timing between right and left appendages, was observed at pressures of > 20 MPa. Our results indicate that the limit of pressure for sustaining active behavior of A. franciscana larvae is ∼20 MPa, whereas the limit of pressure for survival is within the range 30-60 MPa. Thus, members of the genus Artemia possess the ability to resist a higher range of pressures than their natural habitat depth. Our findings demonstrated an example of an organism capable of invading deeper environment in terms of physical pressure tolerance, and indicate the need and importance of pressure study as an experimental method.

  8. Optimizing the co-feeding strategy of Persian sturgeon (Acipenser persicus larvae using Artemia nauplii and formulated diet

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    Naser Agh

    2013-07-01

    Full Text Available High mortality and labor costs are associated with first-feeding sturgeon culture, particularly during the period of dietary transition from live to formulated feed. Therefore we investigated the effects of various feeding treatments on the survival and growth of the Persian sturgeon (Acipenser persicus larvae during a 20-day culture period. Three replicate groups (250 fish/replicate of first-feeding larvae were fed according to four main feeding regimes: (1 live food (live nauplii of brine shrimp Artemia urmiana; (2 indirect transition (5 days live food followed by gradual transition to formulated diet; (3 direct transition (using different combinations of live and formulated diet from the start feeding onwards; (4 formulated feed (FD from the start feeding. Results indicated that growth and survival were higher in the indirect transition feeding regime than in other regimes. Based on our study, co-feeding of A. persicus should start five days after prior feeding with live food.

  9. May arsenic pollution contribute to limiting Artemia franciscana invasion in southern Spain?

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    Marta I. Sánchez

    2016-02-01

    Full Text Available Limited information exists regarding the complex interactions between biological invasions, pollution, and climate change. Most studies indicate that pollution tends to favor invasive species. Here, we provide evidence that arsenic (As pollution may have a role in limiting the invasion of the exotic brine shrimp Artemia franciscana. We tested As toxicity in natural populations of Artemia parthenogenetica (a native taxon and A. franciscana from localities in southern Spain with differing degrees of As contamination. Tests were conducted both under current mean temperature conditions (25 °C, and as per a future climate scenario (i.e., an increase in mean temperature of 4 °C. Acute toxicity was estimated on the basis of the median lethal concentration (at 24 h, and chronic toxicity was evaluated by measuring Artemia survival and growth under sublethal exposures (after 26 days. At 25 °C, native A. parthenogenetica from the highly polluted Odiel and Tinto estuary was much more resistant to acute As stress (LC50-24 h, 24.67 mg L−1 than A. franciscana (15.78 mg L−1 and A. parthenogenetica from unpolluted sites (12.04 mg L−1–suggesting that local adaptation to polluted conditions may occur. At 29 °C, resistance of A. parthenogenetica from Odiel decreased significantly, and there were no statistical differences in sensitivity between the three species/populations, suggesting that climate change may enhance the probability of invasion. Resistance increased with developmental stage from nauplii to adults, and was extremely high in cysts which still hatched at As concentrations of up to 6400 mg L−1. Under sublethal chronic exposure A. franciscana performed better (survival and growth than A. parthenogenetica, and both species experienced a faster growth when exposed to As, compared with unexposed (control individuals, probably due to the hormesis. We discuss the ecological implications of our results.

  10. Artemia (Crustacea, Anostraca in Chile: a review of basic and applied biology Artemia Crustacea, Anostraca en Chile: revisión de la biología básica y aplicada

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    Patricio De los Rios-Escalante

    2012-09-01

    Full Text Available The brine shrimp Artemia in Chile has been studied since the 1980s, initially on populations inhabiting shallow coastal and inland mountain ponds, and saltworks in northern and central Chile. Based on morphometric and molecular evidence, these populations were identified as A. franciscana. In the 1990s, A. persimilis was recorded from southern Patagonia, a species previously considered endemic to Argentina. Recently, two new populations of A.franciscana have been recorded, from one saline coastal pond in northern Chile and from a saltwork in central Chile. The scope for further research to increase both understanding of the strain characterization and basic population ecology descriptions of the Chilean brine shrimps and improve their conservation status is discussed. It is suggested that future studies should investigate first the management of local brine shrimp population for local aquaculture or conservation resources, other direction would be the effects of ultraviolet radiation (UVR exposition that is notoriously high in brine shrimp habitats. This last factor is very important because the UVR is an important mutagen on the genetic structure of the populations. In this scenario, it is suggest a carefully management for introduced brine shrimp populations for local aquaculture for avoid alterations in native populations that due their genetic isolation would need conservation procedures for avoid local extinctions.El camarón de salmuera o Artemia ha sido estudiado en Chile desde la década de 1980, las primeras descripciones de poblaciones fueron para lagunas someras en zonas costeras y de montana, y en salinas artificiales en la zona central y norte de Chile. Sobre la base de evidencias morfométricas y moleculares estas poblaciones fueron descritas como A. franciscana. En la década de 1990, se describió la presencia de A. persimilis en la zona sur de la Patagonia, lo cual fue una ampliación del rango de distribución significativa

  11. Screening of Indian medicinal plants for cytotoxic activity by Brine Shrimp Lethality (BSL assay and evaluation of their total phenolic content

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    Mahesh Biradi

    2014-01-01

    Full Text Available Objective: Plant-derived cytotoxic constituents and polyphenolic compounds have played an important role in the development of clinically useful anticancer agents. In this context, we have selected six Indian medicinal plants based on the literature claims and an attempt was made to evaluate the cytotoxic potential and total phenolic content (TPC of their methanol extracts and fractions. Materials and Methods: Six plants have been selected for the study, namely, Artemisia absinthium Linn. (Asteraceae, Oroxylum indicum (Linn. Vent. (Bignoniaceae, Heliotropium indicum Linn. (Boraginaceae, Amorphophallus sylvaticus (Roxb. Kunth. (Araceae, Mimosa pudica Linn. (Mimosaceae, and Premna serratifolia Linn. (Verbenaceae. Authenticated plant materials were subjected to extraction with methanol by cold maceration and hot percolation methods. The extracts were fractionated into four fractions (F1, F2, F3, and F4. Preliminary phytochemical investigation was carried out for all extracts and fractions. All extracts and their fractions were subjected to cytotoxicity screening by brine shrimp lethality (BSL bioassay. The plants with significant cytotoxicity were evaluated for TPC by using Folin-Ciocalteu reagent. Results: F1, F2, and F3 fractions of A. absinthium and P. serratifolia and F1 fraction of M. pudica have shown significant cytotoxicity (lethal concentration (LC 50 < 100 ppm compared with other fractions. F1, F2, and F3 fractions of A. absinthium show the LC 50 values 32.52, 14.27, and 24.02, respectively; F1, F2, and F3 of P. serratifolia show LC 50 values 7.61, 4.01, and 10.91 and same for F1 fraction of M. pudica was 34.82 μg/ml, respectively. TPC was found to be significantly higher (39.11 mg gallic acid equivalent (GAE/g in P. serratifolia compared with other two plants. Conclusion: The cytotoxicity screening system confirmed the proposed anticancer plants used by traditional healers and literature claims.

  12. Identifikasi Metabolit Sekunder Ekstrak Etil Asetat Biji Alpukat (Persea americana Mill. dan Uji Toksisitas Terhadap Larva Udang Artemia salina Leach.

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    Nurdia Asdar

    2014-07-01

    Full Text Available Identification of metabolite compound in acetone Extract from Fruits Avocado seed (Percea americana Mill and toxicity test against Artemia salina Leach. Aim of this research is to identify the secondary metabolite compound in aceton extract from fruits Avocado seed (Percea americana Mill and to determine the potential of secondary metabolites from an avocado extract as anticancer. The compound was obtained from Identification process in several stages, namely extraction, fractination, purification and identification. The identification process was color test, TLC, spectroscopy IR. The toxicity test by Brine Shrimp Lethality Test (BSLT to Artemia salina Leach. The compound obtained from this research was yellow-white crystalline needle-shaped, the purity test with TLC analysis showed a stain in three eluen system on the stain test on three eluent systems with Rf proportions as follows; 0,15 from chloroform:ethyl acetat (6:4, 0,33 from methanol:chloroform (1:9, 0,75 for acetone:ethyl acetat, and positively to the reagent Wagner and gave brown precipitate for indicate as alcaloid group. This result is supported by spectroscopy from compound.While toxicity tests showed condensed acetone extracts  and pure compounds are toxic with LC50 value of each is 20.61 mg/mL and 39,81 mg/mL.

  13. Acclimation and tolerance of Artemia salina to copper salts

    Energy Technology Data Exchange (ETDEWEB)

    Saliba, L.J.; Krzyz, R.M.

    1976-01-01

    The brine shrimp Artemia salina L. was acclimated in sea water with cupric chloride, acetate, carbonate, and sulfate, each at concentrations of 0.1, 0.05 and 0.025 ppM Cu/sup + +/, together with sea water controls. Growth inhibition was observed in all four compounds, generally in direct relationship to the concentration. It was least in sulfate, and increased progressively in chloride, acetate and carbonate in that order. In toxicity tests, 2-week old larvae from each solution were exposed to concentrations of 10, 7.5, 5, 2.5 and 1 ppM Cu/sup + +/ of the same compounds, together with unacclimated larvae of the same age. Similar tests were held with 6-week old adults. Toxicity to unacclimated larvae and adults differed with the compounds, carbonate being the least toxic, followed by sulfate, chloride and acetate in increasing order. Larvae acclimated in chloride and sulfate showed an increased tolerance to 1 and 2.5 ppM Cu/sup + +/ compared to untreated controls. Tolerance was not enhanced from 5 ppM Cu/sup + +/ upwards. In both compounds, adults acclimated in 0.1 ppM Cu/sup + +/ showed an increased tolerance to concentrations between 1 and 7.5 ppM Cu/sup + +/ compared to controls. Considerable precipitation occurred with the high levels of this compound, thus effecting the ''final'' concentrations. No acclimation effect was observed in acetate for either larvae or adults. It is suggested that in A. salina, copper toxicity depends on the particular form of the metal, and that this difference is also evident in growth inhibition and in the potential acquisition of increased tolerance through exposure to low concentrations.

  14. Long term exposure to low dose neurotoxic pesticides affects hatching, viability and cholinesterase activity of Artemia sp.

    Science.gov (United States)

    Gambardella, Chiara; Nichino, Daniela; Iacometti, Camillo; Ferrando, Sara; Falugi, Carla; Faimali, Marco

    2018-03-01

    The brine shrimp Artemia was used as a model organism to test toxicity of several neuroactive pesticides (chlorpyrifos (CLP), chlorpyrifos oxon (CLP ox), diazinon (DZN), carbaryl (CBR)) following exposure to far below than lethal doses. Cysts were exposed to the pesticides in order to test a scenario similar to actual coastal environment contamination, by analyzing different responses. Cysts were rehydrated in water containing the pesticides at concentrations ranging from 10 -11 to 10 -5  M, for 72, 96 and 192 h, respectively. For these exposure times, morpho-functional and biochemical parameters, such as hatching speed and viability were investigated in the larvae together with cholinesterase (ChE) activity quantification and histochemical localization. Finally, ChE inhibition was also compared with conventional selective ChE inhibitors. Results showed that CLP ox and CBR caused a significant dose-dependent decrease in hatching speed, followed by high percentages of larval death, while CLP and DZN were responsible for irregular hatching patterns. In addition, the pesticides mostly caused larval death some days post-hatching, whereas this effect was negligible for the specific ChE inhibitors, suggesting that part of pesticide toxicity may be due to molecules other than the primary target. ChE activity was observed in the protocerebrum lobes, linked to the development of pair eyes. Such activity was inhibited in larvae exposed to all pesticides. When compared to conventional selective inhibitors of ChE activities, this inhibition demonstrated that the selected pesticides mainly affect acetylcholinesterase and, to a lesser extent, pseudocholinesterases. In conclusion, the brine shrimp is a good model to test the environmental toxicity of long term exposure to cholinergic pesticides, since changes in hatching speed, viability and ChE activity were observed. Copyright © 2018 Elsevier B.V. All rights reserved.

  15. Determination of biological and physicochemical parameters of Artemia franciscana strains in hypersaline environments for aquaculture in the Colombian Caribbean.

    Science.gov (United States)

    Camargo, William N; Durán, Gabriel C; Rada, Orlando C; Hernández, Licet C; Linero, Juan-Carlos G; Muelle, Igor M; Sorgeloos, Patrick

    2005-10-26

    Artemia (Crustacea, Anostraca), also known as brine shrimp, are typical inhabitants of extreme environments. These hypersaline environments vary considerably in their physicochemical composition, and even their climatic conditions and elevation. Several thalassohaline (marine) environments along the Colombian Caribbean coast were surveyed in order to contribute to the knowledge of brine shrimp biotopes in South America by determining some vital biological and physicochemical parameters for Artemia survival. Additionally, cyst quality tests, biometrical and essential fatty acids analysis were performed to evaluate the economic viability of some of these strains for the aquaculture industry. In addition to the three locations (Galerazamba, Manaure, and Pozos Colorados) reported in the literature three decades ago in the Colombian Caribbean, six new locations were registered (Salina Cero, Kangaru, Tayrona, Bahía Hondita, Warrego and Pusheo). All habitats sampled showed that chloride was the prevailing anion, as expected, because of their thalassohaline origin. There were significant differences in cyst diameter grouping strains in the following manner according to this parameter: 1) San Francisco Bay (SFB-Control, USA), 2) Galerazamba and Tayrona, 3) Kangarú, 4) Manaure, and 5) Salina Cero and Pozos Colorados. Chorion thickness values were smaller in Tayrona, followed by Salina Cero, Galerazamba, Manaure, SFB, Kangarú and Pozos Colorados. There were significant differences in naupliar size, grouping strains as follows (smallest to largest): 1) Galerazamba, 2) Manaure, 3) SFB, Kangarú, and Salina Cero, 4) Pozos Colorados, and 5) Tayrona. Overall, cyst quality analysis conducted on samples from Manaure, Galerazamba, and Salina Cero revealed that all sites exhibited a relatively high number of cysts.g-1. Essential fatty acids (EFA) analysis performed on nauplii from cyst samples from Manaure, Galerazamba, Salina Cero and Tayrona revealed that cysts from all sites

  16. Virulence of luminous vibrios to Artemia franciscana nauplii.

    Science.gov (United States)

    Soto-Rodriguez, S A; Roque, A; Lizarraga-Partida, M L; Guerra-Flores, A L; Gomez-Gill, B

    2003-02-27

    From healthy and diseased penaeid shrimp from Asia and the Americas, 25 luminous and 2 non-luminous bacterial strains were isolated, and 14 were phenotypically identified as Vibrio harveyi; 9 isolates produced significant mortalities (45 to 80%) in Artemia franciscana nauplii at inoculation densities of 10(5) to 10(6) CFU ml(-1) compared to the controls (unchallenged nauplii). The maximum number of bacteria ingested (bioencapsulated) by the Artemia nauplii varied from less than 10 to 10(3) CFU nauplius(-1) and no significant relationship was observed between the density of bacteria inoculated, the amount of bacteria ingested, and naupliar mortality. Significant correlations were obtained between naupliar mortality and production of proteases, phospholipases or siderophores, but not between mortality and lipase production, gelatinase production, hydrophobicity or hemolytic activity. The results suggest that virulence of the strains tested was more related to the production of particular exoenzymes than to the measured colonization factors.

  17. Primary Screening of the Bioactivity of Brackishwater Cyanobacteria: Toxicity of Crude Extracts to Artemia salina Larvae and Paracentrotus lividus Embryos

    Directory of Open Access Journals (Sweden)

    Viviana R. Lopes

    2010-03-01

    Full Text Available Cyanobacteria are a diverse group of Gram-negative bacteria that produce an array of secondary compounds with selective bioactivity against vertebrates, invertebrates, plants, microalgae, fungi, bacteria, viruses and cell lines. The aim of this study was to assess the toxic effects of aqueous, methanolic and hexane crude extracts of benthic and picoplanktonic cyanobacteria isolated from estuarine environments, towards the nauplii of the brine shrimp Artemia salina and embryos of the sea urchin Paracentrotus lividus. The A. salina lethality test was used as a frontline screen and then complemented by the more specific sea urchin embryo-larval assay. Eighteen cyanobacterial isolates, belonging to the genera Cyanobium, Leptolyngbya, Microcoleus, Phormidium, Nodularia, Nostoc and Synechocystis, were tested. Aqueous extracts of cyanobacteria strains showed potent toxicity against A. salina, whereas in P. lividus, methanolic and aqueous extracts showed embryo toxicity, with clear effects on development during early stages. The results suggest that the brackishwater cyanobacteria are producers of bioactive compounds with toxicological effects that may interfere with the dynamics of invertebrate populations.

  18. Effects of selected metal oxide nanoparticles on Artemia salina larvae: evaluation of mortality and behavioural and biochemical responses.

    Science.gov (United States)

    Gambardella, Chiara; Mesarič, Tina; Milivojević, Tamara; Sepčić, Kristina; Gallus, Lorenzo; Carbone, Serena; Ferrando, Sara; Faimali, Marco

    2014-07-01

    The aim was to investigate the toxicity of selected metal oxide nanoparticles (MO-NPs) on the brine shrimp Artemia salina, by evaluating mortality and behavioural and biochemical responses. Larvae were exposed to tin(IV) oxide (stannic oxide (SnO2)), cerium(IV) oxide (CeO2) and iron(II, III) oxide (Fe3O4) NPs for 48 h in seawater, with MO-NP suspensions from 0.01 to 1.0 mg/mL. Mortality and behavioural responses (swimming speed alteration) and enzymatic activities of cholinesterase, glutathione-S-transferase and catalase were evaluated. Although the MO-NPs did not induce any mortality of the larvae, they caused changes in behavioural and biochemical responses. Swimming speed significantly decreased in larvae exposed to CeO2 NPs. Cholinesterase and glutathione-S-transferase activities were significantly inhibited in larvae exposed to SnO2 NPs, whereas cholinesterase activity significantly increased after CeO2 NP and Fe3O4 NP exposure. Catalase activity significantly increased in larvae exposed to Fe3O4 NPs. In conclusion, swimming alteration and cholinesterase activity represent valid endpoints for MO-NP exposure, while glutathione-S-transferase and catalase activities appear to be NP-specific.

  19. Does a Change from Whole to Powdered Food (Artemia franciscana eggs Increase Oviposition in the Ladybird Coleomegilla maculata?

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    Eric W. Riddick

    2015-09-01

    Full Text Available The limited availability of alternative foods to replace natural prey hinders cost-effective mass production of ladybird beetles for augmentative biological control. We compared the effects of powdered vs. whole Artemia franciscana (A. franciscana (brine shrimp eggs with or without a dietary supplement on development and reproduction of Coleomegilla maculata (C. maculata (Coleoptera: Coccinellidae. We tested the hypotheses that (1 powdered A. franciscana eggs are more suitable than whole eggs; and (2 palmitic acid, a common fatty acid in natural prey, i.e., aphids, is an effective dietary supplement. Development time, pre-imaginal survival, sex ratio, and body weight of adults did not differ significantly amongst individuals fed powdered vs. whole eggs, with or without 5% palmitic acid. Significantly more oviposition occurred when females were fed powdered eggs than whole eggs and powdered eggs with 5% palmitic acid than whole eggs with or without 5% palmitic acid. A weak functional relationship was found between pre-oviposition time and total oviposition by females fed powdered eggs with 5% palmitic acid; pre-oviposition time decreased as oviposition increased. Food treatments had no significant differential effect on progeny (egg hatch rate. In conclusion, a simple change in A. franciscana egg texture and particle size (i.e., blending whole eggs into a dust-like powder increases oviposition in C. maculata. Supplementing powdered eggs with 5% palmitic acid might accelerate oogenesis (egg maturation in some females.

  20. Predicted sub-populations in a marine shrimp proteome as revealed by combined EST and cDNA data from multiple Penaeus species

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    Kotewong Rattanawadee

    2010-11-01

    Full Text Available Abstract Background Many species of marine shrimp in the Family Penaeidae, viz. Penaeus (Litopenaeus vannamei, Penaeus monodon, Penaeus (Fenneropenaeus chinensis, and Penaeus (Marsupenaeus japonicus, are animals of economic importance in the aquaculture industry. Yet information about their DNA and protein sequences is lacking. In order to predict their collective proteome, we combined over 270,000 available EST and cDNA sequences from the 4 shrimp species with all protein sequences of Drosophila melanogaster and Caenorhabditis elegans. EST data from 4 other crustaceans, the crab Carcinus maenas, the lobster Homarus americanus (Decapoda, the water flea Daphnia pulex, and the brine shrimp Artemia franciscana were also used. Findings Similarity searches from EST collections of the 4 shrimp species matched 64% of the protein sequences of the fruit fly, but only 45% of nematode proteins, indicating that the shrimp proteome content is more similar to that of an insect than a nematode. Combined results with 4 additional non-shrimp crustaceans increased matching to 78% of fruit fly and 56% of nematode proteins, suggesting that present shrimp EST collections still lack sequences for many conserved crustacean proteins. Analysis of matching data revealed the presence of 4 EST groups from shrimp, namely sequences for proteins that are both fruit fly-like and nematode-like, fruit fly-like only, nematode-like only, and non-matching. Gene ontology profiles of proteins for the 3 matching EST groups were analyzed. For non-matching ESTs, a small fraction matched protein sequences from other species in the UniProt database, including other crustacean-specific proteins. Conclusions Shrimp ESTs indicated that the shrimp proteome is comprised of sub-populations of proteins similar to those common to both insect and nematode models, those present specifically in either model, or neither. Combining small EST collections from related species to compensate for their

  1. Estudo fitoquímico preliminar e bioensaio toxicológico frente a larvas de Artemia salina Leach. de extrato obtido de frutos de Solanum lycocarpum A. St.-Hill (Solanaceae

    Directory of Open Access Journals (Sweden)

    Marcelo Gonzaga de Freitas Araújo

    2010-11-01

    Full Text Available

    Neste trabalho avaliou-se o perfil fitoquímico e a toxicidade preliminar frente a larvas de Artemia salina do extrato etanólico de frutos de Solanum lycocarpum. O extrato foi submetido à analise fitoquímica preliminar para identificação das principais classes de metabolitos secundários presentes e testado frente a larvas de A. salina para obtenção das concentrações letais médias (CL50%. Os testes fitoquímicos demonstraram a presença de fenóis, taninos, saponinas, alcalóides e esteróides e triterpenos livres. O extrato foi fracionado em diferentes solventes para a avaliação da toxicidade frente à A. salina, apresentando considerável citotoxicidade encontrada na fração hidroalcoólica (CL50% = 285,546 µg/mL. Palavras-chave: Solanum lycocarpum, Artemia salina, triagem fitoquímica preliminar. ABSTRACT The phytochemical profile of ethanolic extract of Solanum lycocarpum fruits was analyzed and preliminary toxicity tests were performed against brine shrimp larvae. The extract was subjected to preliminary phytochemical analysis to identify the main classes of secondary metabolites and tested against the larvae of A. salina to obtain the median lethal concentrations (LC50%. The phytochemical tests showed the presence of phenols, tannins, saponins, alkaloids and free steroids. The extract was fractionated with various solvents for toxicity testing against the larvae and the hydroalcoholic fraction showed considerable cytotoxicity (CL50% = 285.546 g/mL. Keywords: Solanum lycocarpum, Artemia salina, phytochemical screening

  2. Assessment of Euphorbia hirta L. Leaf, Flower, Stem and Root Extracts for Their Antibacterial and Antifungal Activity and Brine Shrimp Lethality

    Directory of Open Access Journals (Sweden)

    Santhanam Amutha

    2010-08-01

    zones ranged between 16–29 mm. Leaves extract inhibited the growth of all tested microorganisms with large zones of inhibition, followed by that of flowers, which also inhibited all the bacteria except C. albicans. The most susceptible microbes to all extracts were S. aureus and Micrococcus sp. Root extract displayed larger inhibition zones against Gram positive bacteria than Gram negative bacteria and had larger inhibition zones compared to stem extract. The lowest MIC values were obtained with E. coli and C. albicans (3.12 mg/mL, followed by S. aureus (12.50 mg/mL and P. mirabilis (50.00 mg/mL. All the other bacteria had MIC values of 100.00 mg/mL. Scanning Electron Microscopic (SEM studies revealed that the cells exposed to leaf extract displayed a rough surface with multiple blends and invaginations which increased with increasing time of treatment, and cells exposed to leaf extract for 36 h showed the most damage, with abundant surface cracks which may be related to final cell collpase and loss of function. Time-kill assay of C. albicans indicated a primarily fungicidal effect at 1- and 2-fold MIC. E. hirta extracts had LC50 values of 0.71, 0.66, 0.41 and 0.03 mg/mL for stems, leaves, roots and flowers, respectively against Artemia salina. Hence, these plants can be used to discover new bioactive natural products that may serve as leads in the development of new pharmaceuticals.

  3. High surface adsorption properties of carbon-based nanomaterials are responsible for mortality, swimming inhibition, and biochemical responses in Artemia salina larvae.

    Science.gov (United States)

    Mesarič, Tina; Gambardella, Chiara; Milivojević, Tamara; Faimali, Marco; Drobne, Damjana; Falugi, Carla; Makovec, Darko; Jemec, Anita; Sepčić, Kristina

    2015-06-01

    We investigated the effects of three different carbon-based nanomaterials on brine shrimp (Artemia salina) larvae. The larvae were exposed to different concentrations of carbon black, graphene oxide, and multiwall carbon nanotubes for 48 h, and observed using phase contrast and scanning electron microscopy. Acute (mortality) and behavioural (swimming speed alteration) responses and cholinesterase, glutathione-S-transferase and catalase enzyme activities were evaluated. These nanomaterials were ingested and concentrated in the gut, and attached onto the body surface of the A. salina larvae. This attachment was responsible for concentration-dependent inhibition of larval swimming, and partly for alterations in the enzyme activities, that differed according to the type of tested nanomaterials. No lethal effects were observed up to 0.5mg/mL carbon black and 0.1mg/mL multiwall carbon nanotubes, while graphene oxide showed a threshold whereby it had no effects at 0.6 mg/mL, and more than 90% mortality at 0.7 mg/mL. Risk quotients calculated on the basis of predicted environmental concentrations indicate that carbon black and multiwall carbon nanotubes currently do not pose a serious risk to the marine environment, however if uncontrolled release of nanomaterials continues, this scenario can rapidly change. Copyright © 2015 Elsevier B.V. All rights reserved.

  4. High surface adsorption properties of carbon-based nanomaterials are responsible for mortality, swimming inhibition, and biochemical responses in Artemia salina larvae

    International Nuclear Information System (INIS)

    Mesarič, Tina; Gambardella, Chiara; Milivojević, Tamara; Faimali, Marco; Drobne, Damjana; Falugi, Carla; Makovec, Darko; Jemec, Anita; Sepčić, Kristina

    2015-01-01

    Highlights: • Carbon-based nanomaterials adsorb onto the body surface of A. salina larvae. • Surface adsorption results in concentration–dependent inhibition of larval swimming. • Carbon-based nanomaterials induce no significant mortality of A. salina larvae. - Abstract: We investigated the effects of three different carbon-based nanomaterials on brine shrimp (Artemia salina) larvae. The larvae were exposed to different concentrations of carbon black, graphene oxide, and multiwall carbon nanotubes for 48 h, and observed using phase contrast and scanning electron microscopy. Acute (mortality) and behavioural (swimming speed alteration) responses and cholinesterase, glutathione-S-transferase and catalase enzyme activities were evaluated. These nanomaterials were ingested and concentrated in the gut, and attached onto the body surface of the A. salina larvae. This attachment was responsible for concentration–dependent inhibition of larval swimming, and partly for alterations in the enzyme activities, that differed according to the type of tested nanomaterials. No lethal effects were observed up to 0.5 mg/mL carbon black and 0.1 mg/mL multiwall carbon nanotubes, while graphene oxide showed a threshold whereby it had no effects at 0.6 mg/mL, and more than 90% mortality at 0.7 mg/mL. Risk quotients calculated on the basis of predicted environmental concentrations indicate that carbon black and multiwall carbon nanotubes currently do not pose a serious risk to the marine environment, however if uncontrolled release of nanomaterials continues, this scenario can rapidly change

  5. High surface adsorption properties of carbon-based nanomaterials are responsible for mortality, swimming inhibition, and biochemical responses in Artemia salina larvae

    Energy Technology Data Exchange (ETDEWEB)

    Mesarič, Tina, E-mail: tina.mesaric84@gmail.com [Department of Biology, Biotechnical Faculty, University of Ljubljana (Slovenia); Gambardella, Chiara, E-mail: chiara.gambardella@ge.ismar.cnr.it [Institute of Marine Sciences, National Research Council, Genova (Italy); Milivojević, Tamara, E-mail: milivojevictamara@gmail.com [Department of Biology, Biotechnical Faculty, University of Ljubljana (Slovenia); Faimali, Marco, E-mail: marco.faimali@ismar.cnr.it [Institute of Marine Sciences, National Research Council, Genova (Italy); Drobne, Damjana, E-mail: damjana.drobne@bf.uni-lj.si [Department of Biology, Biotechnical Faculty, University of Ljubljana (Slovenia); Centre of Excellence in Nanoscience and Nanotechnology (CO Nanocentre), Ljubljana (Slovenia); Centre of Excellence in Advanced Materials and Technologies for the Future (CO NAMASTE), Ljubljana (Slovenia); Falugi, Carla, E-mail: carlafalugi@hotmail.it [Department of Earth, Environment and Life Sciences, University of Genova, Genova (Italy); Makovec, Darko, E-mail: darko.makovec@ijs.si [Jožef Stefan Institute, Jamova 39, 1000 Ljubljana (Slovenia); Jemec, Anita, E-mail: anita.jemec@bf.uni-lj.si [Department of Biology, Biotechnical Faculty, University of Ljubljana (Slovenia); Sepčić, Kristina, E-mail: kristina.sepcic@bf.uni-lj.si [Department of Biology, Biotechnical Faculty, University of Ljubljana (Slovenia)

    2015-06-15

    Highlights: • Carbon-based nanomaterials adsorb onto the body surface of A. salina larvae. • Surface adsorption results in concentration–dependent inhibition of larval swimming. • Carbon-based nanomaterials induce no significant mortality of A. salina larvae. - Abstract: We investigated the effects of three different carbon-based nanomaterials on brine shrimp (Artemia salina) larvae. The larvae were exposed to different concentrations of carbon black, graphene oxide, and multiwall carbon nanotubes for 48 h, and observed using phase contrast and scanning electron microscopy. Acute (mortality) and behavioural (swimming speed alteration) responses and cholinesterase, glutathione-S-transferase and catalase enzyme activities were evaluated. These nanomaterials were ingested and concentrated in the gut, and attached onto the body surface of the A. salina larvae. This attachment was responsible for concentration–dependent inhibition of larval swimming, and partly for alterations in the enzyme activities, that differed according to the type of tested nanomaterials. No lethal effects were observed up to 0.5 mg/mL carbon black and 0.1 mg/mL multiwall carbon nanotubes, while graphene oxide showed a threshold whereby it had no effects at 0.6 mg/mL, and more than 90% mortality at 0.7 mg/mL. Risk quotients calculated on the basis of predicted environmental concentrations indicate that carbon black and multiwall carbon nanotubes currently do not pose a serious risk to the marine environment, however if uncontrolled release of nanomaterials continues, this scenario can rapidly change.

  6. Complexity in a population of Artemia

    Energy Technology Data Exchange (ETDEWEB)

    Ali, A.A., E-mail: abduladem1@yahoo.co [Electrical Engineering Department, University of Basrah (Iraq); Fortuna, L., E-mail: lfortuna@diees.unict.i [DIEEI, Faculty of Engineering, University of Catania (Italy); Frasca, M., E-mail: mfrasca@diees.unict.i [DIEEI, Faculty of Engineering, University of Catania (Italy); Rashid, M.T., E-mail: mofid76@yahoo.co [Electrical Engineering Department, University of Basrah (Iraq); Xibilia, M.G., E-mail: mxibilia@ingegneria.unime.i [DiSIA, Faculty of Engineering, University of Messina (Italy)

    2011-04-15

    Highlights: Experiments on collective motion of populations of animals (Artemia salina). Design of low-cost experimental setup for complex systems. Control of collective motion of populations of Artemia. Models of collective motion of populations of Artemia. - Abstract: Artemia salina belongs to a genus of very primordial crustaceans, whose behavior is not widely investigated in literature. Their collective behavior is studied in this paper both experimentally and theoretically. Different experiments have been designed to control the direction of motion of an Artemia population by exploiting their sensitivity to light and to measure the response of the population to light at different wavelengths. Mathematical models have been also derived, explaining the mechanisms underlying Artemia flocking formation when a light spot is applied to the system. The results obtained allow to develop new strategies for distributed control of agents and to test them in a simple and low cost experimental setup.

  7. Complexity in a population of Artemia

    International Nuclear Information System (INIS)

    Ali, A.A.; Fortuna, L.; Frasca, M.; Rashid, M.T.; Xibilia, M.G.

    2011-01-01

    Highlights: → Experiments on collective motion of populations of animals (Artemia salina). → Design of low-cost experimental setup for complex systems. → Control of collective motion of populations of Artemia. → Models of collective motion of populations of Artemia. - Abstract: Artemia salina belongs to a genus of very primordial crustaceans, whose behavior is not widely investigated in literature. Their collective behavior is studied in this paper both experimentally and theoretically. Different experiments have been designed to control the direction of motion of an Artemia population by exploiting their sensitivity to light and to measure the response of the population to light at different wavelengths. Mathematical models have been also derived, explaining the mechanisms underlying Artemia flocking formation when a light spot is applied to the system. The results obtained allow to develop new strategies for distributed control of agents and to test them in a simple and low cost experimental setup.

  8. Forward Osmosis Brine Drying

    Science.gov (United States)

    Flynn, Michael; Shaw, Hali; Hyde, Deirdre; Beeler, David; Parodi, Jurek

    2015-01-01

    The Forward Osmosis Brine Drying (FOBD) system is based on a technique called forward osmosis (FO). FO is a membrane-based process where the osmotic potential between brine and a salt solution is equalized by the movement of water from the brine to the salt solution. The FOBD system is composed of two main elements, the FO bag and the salt regeneration system. This paper discusses the results of testing of the FO bag to determine the maximum water recovery ratio that can be attained using this technology. Testing demonstrated that the FO bag is capable of achieving a maximum brine water recovery ratio of the brine of 95%. The equivalent system mass was calculated to be 95 kg for a feed similar to the concentrated brine generated on the International Space Station and 86 kg for an Exploration brine. The results have indicated that the FOBD can process all the brine for a one year mission for between 11% to 10% mass required to bring the water needed to make up for water lost in the brine if not recycled. The FOBD saves 685 kg and when treating the International Space Station brine and it saves 829 kg when treating the Exploration brine. It was also demonstrated that saturated salt solutions achieve a higher water recovery ratios than solids salts do and that lithium chloride achieved a higher water recovery ratio than sodium chloride.

  9. Development and validation of an extensive growth and growth boundary model for Listeria monocytogenes in lightly preserved and ready-to-eat shrimp

    DEFF Research Database (Denmark)

    Mejlholm, Ole; Dalgaard, Paw

    2009-01-01

    and their interactive effects. The new model predicted growth rates (mu(max) values) of L. monocytogenes accurately with bias and accuracy factors of 1.0 and 1.5, respectively, for 16 batches of brined shrimp with benzoic, citric, and sorbic acids. Corresponding values of 0.9 and 1.2, respectively, were obtained...... for five batches of brined shrimp with acetic and lactic acids. Growth and no-growth responses of L. monocytogenes were also appropriately predicted with 88% correct prediction for 26 experiments with brined shrimp. The new model performed better than existing L. monocytogenes models with a comparable...

  10. Results for the Brine Evaporation Bag (BEB) Brine Processing Test

    Science.gov (United States)

    Delzeit, Lance; Flynn, Michael; Fisher, John; Shaw, Hali; Kawashima, Brian; Beeler, David; Howard, Kevin

    2015-01-01

    The recent Brine Processing Test compared the NASA Forward Osmosis Brine Dewatering (FOBD), Paragon Ionomer Water Processor (IWP), UMPQUA Ultrasonic Brine Dewatering System (UBDS), and the NASA Brine Evaporation Bag (BEB). This paper reports the results of the BEB. The BEB was operated at 70 deg C and a base pressure of 12 torr. The BEB was operated in a batch mode, and processed 0.4L of brine per batch. Two different brine feeds were tested, a chromic acid-urine brine and a chromic acid-urine-hygiene mix brine. The chromic acid-urine brine, known as the ISS Alternate Pretreatment Brine, had an average processing rate of 95 mL/hr with a specific power of 5kWhr/L. The complete results of these tests will be reported within this paper.

  11. [Concentrations of alkaloids, cyanogenic glycosides, polyphenols and saponins in selected medicinal plants from Ecuador and their relationship with acute toxicity against Artemia salina].

    Science.gov (United States)

    Jaramillo Jaramillo, Carmita; Jaramillo Espinoza, Anyi; D'Armas, Haydelba; Troccoli, Luis; Rojas de Astudillo, Luisa

    2016-09-01

    containing low concentrations of chemical compounds with high polarities. The significant positive correlation between cytotoxicity and concentration of alkaloids confirmed by the bioassay of brine shrimp can be useful to identify promising sources of antitumor compounds, and to evaluate tolerable limits not affecting other benign cells. Contents of secondary metabolites found in the selected plants confer them great pharmacologic values.

  12. The effects of different routes of inulin administration on gut microbiota and survival rate of Indian white shrimp post-larvae (Fenneropenaeus indicus).

    Science.gov (United States)

    Hoseinifar, Seyed Hossein; Zare, Parviz; Kolangi Miandare, Hamed

    2015-01-01

    The present study investigates the effects of different routes of inulin administration as prebiotic on gut microbiota and survival rate of Indian white shrimp post-larvae. Four hundred and fifty Indian white shrimp post-larvae (PL1) were stocked in nine tanks. The tanks were assigned into three treatments: feeding with inulin-treated (110 mg L(-1)) Artemia nauplii (I-T), feeding with inulin-enriched (110 mg L(-1)) Artemia nauplii (I-E) and control which repeated triplicates. Feeding trial was performed until PL11 stage and then gut microbiota was studied using culture based method. Also, survival rate was calculated at the end of feeding trial. Our results showed that feeding on inulin enriched or treated Artemia nauplii had no significant effect on total viable culturable autochthonous bacteria and Vibrio spp. levels of the gut microbiota (p > 0.05). However, a remarkable increase of lactic acid bacteria levels (LAB) was observed in I-E treatment (p shrimp post-larvae (p shrimp but determination the mode of action of prebiotic on various aspects of shrimp larviculture merit further research.

  13. Shrimp Survey Data

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Northern Shrimp Survey was initiated in 1983 by the Atlantic States Marine Fisheries Commission (ASMFC) and monitors the relative abundance (number of shrimp),...

  14. Embriões liofilizados e flocos de Artemia no cultivo pós-larval de Litopenaeus vannamei (Boone, 1931 = Use of freeze-dried embryos and Artemia flakes in the post-larval culture of Litopenaeus vannamei (Boone, 1931

    Directory of Open Access Journals (Sweden)

    Pedro Hercílio de Oliveira Cavalcante

    2008-01-01

    Full Text Available O objetivo do estudo foi avaliar o crescimento e sobrevivência de 1pós-larvas de Litopenaeus vannamei que receberam embriões liofilizados (EMB e flocos (FLO de Artemia como suplementos alimentares a uma dieta microparticulada (DMP. Nos tratamentos T1,T2 e T3, os camarões receberam DMP e após 2h, acréscimos de FLO (T1; de EMB (T2; e da mesma DMP, anteriormente, ofertada (T3. No T4, as pós-larvas foram alimentadas com DMP, mas sem suplementações posteriores. Não houve diferença significativa (p > 0,05 na sobrevivência dos camarões após o período experimental de 21 dias. Por outro lado, o ganho de peso absoluto (mg peso seco das pós-larvas, cuja dieta foi suplementada com BEM, foi significativamente superior (p The objective of the study was to evaluate the growth and survival of Litopenaeus vannamei post-larvae fed with freeze-driedembryos and Artemia flakes as feeding supplements to a microparticulate diet (DMP. In treatments T1, T2 and T3, the shrimp were fed with DMP and after 2 hours, received supplements of FLO (T1; EMB (T2; and the same DMP previously offered (T3. In T4,post-larvae were fed with DMP but without additional supplementation. There was no significant difference (p > 0.05 in shrimp survival after the experimental period of 21 days. On the other hand, the absolute weight gain (mg of dry weight of post-larvae whose dietwas supplemented with EMB (T2 was significantly higher (p < 0.05 than growth found in the other experimental groups (T1, T3 and T4. Shrimp fed with DMP and EMB obtained a significantly higher absolute weight gain (6.7 mg than those found in treatments that used DMP and FLO (5.4 mg or DMP alone (5.8 mg in T3 and 5.7 mg in T4. Theseresults underline the nutritional value of freeze-dried Artemia embryos and recommend their inclusion in the post-larval diet of Litopenaeus vannamei.

  15. Larvicidal activity of some marine macrophytes against Artemia salina

    Digital Repository Service at National Institute of Oceanography (India)

    PrabhaDevi; Wahidullah, S.; DeSouza, L.; Kamat, S.Y.

    and Microdictyon pseudohapteron, seagrasses (Halophila ovalis and Syringodium isoetifolium and lichens (Umbilicaria arpina) were tested for larvicidal activity using Artemia salina nauplii. The LC50 values for the PE-fraction of M. pseudohapteron and A. muscoides...

  16. Embriões liofilizados e flocos de Artemia no cultivo pós-larval de Litopenaeus vannamei (Boone, 1931 - DOI: 10.4025/actascibiolsci.v30i1.1424 Use of freeze-dried embryos and Artemia flakes in the post-larval culture of Litopenaeus vannamei (Boone, 1931 - DOI: 10.4025/actascibiolsci.v30i1.1424

    Directory of Open Access Journals (Sweden)

    Pedro Hercílio de Oliveira Cavalcante

    2008-03-01

    Full Text Available O objetivo do estudo foi avaliar o crescimento e sobrevivência de pós-larvas de Litopenaeus vannamei que receberam embriões liofilizados (EMB e flocos (FLO de Artemia como suplementos alimentares a uma dieta microparticulada (DMP. Nos tratamentos T1, T2 e T3, os camarões receberam DMP e após 2h, acréscimos de FLO (T1; de EMB (T2; e da mesma DMP, anteriormente, ofertada (T3. No T4, as pós-larvas foram alimentadas com DMP, mas sem suplementações posteriores. Não houve diferença significativa (p > 0,05 na sobrevivência dos camarões após o período experimental de 21 dias. Por outro lado, o ganho de peso absoluto (mg peso seco das pós-larvas, cuja dieta foi suplementada com BEM, foi significativamente superior (p Artemia e recomendam sua inclusão na dieta pós-larval de Litopenaeus vannameiThe objective of the study was to evaluate the growth and survival of Litopenaeus vannamei post-larvae fed with freeze-dried embryos and Artemia flakes as feeding supplements to a microparticulate diet (DMP. In treatments T1, T2 and T3, the shrimp were fed with DMP and after 2 hours, received supplements of FLO (T1; EMB (T2; and the same DMP previously offered (T3. In T4, post-larvae were fed with DMP but without additional supplementation. There was no significant difference (p > 0.05 in shrimp survival after the experimental period of 21 days. On the other hand, the absolute weight gain (mg of dry weight of post-larvae whose diet was supplemented with EMB (T2 was significantly higher (p Artemia embryos and recommend their inclusion in the post-larval diet of Litopenaeus vannamei

  17. Antibacterial, antioxidant and cytotoxic activities of extracts from the ...

    African Journals Online (AJOL)

    HALA

    2012-10-11

    Oct 11, 2012 ... capacity of each sample to scavenge the ABTS radical cation, we revealed that the EPS aqueous ... evaluated using the brine shrimp Artemia salina, as test organism. ..... released by cyanobacterium Arthrospira platensis.

  18. Evaluation of Glider Coatings Against Biofouling for Improved Flight Performance

    Science.gov (United States)

    2011-08-17

    and used to conduct assays of survivorship with approximately 100 nauplii larvae ofArtemia sp. (brine shrimp ). The larvae were exposed to the...seal (D). These coatings showed higher mortality of brine shrimp as well as 100% mortality of cypris larvae during the settlement assay which...brine shrimp compared to the glass control; however these did not inhibit settlement and were evidently not toxic to the cypris larvae . All other

  19. Soil washing for brine removal

    International Nuclear Information System (INIS)

    Ayyachamy, J.S.; Atalay, A.; Zaman, M.

    1992-01-01

    During the exploration for oil and thereafter, brine transfer lines get ruptured releasing the brine which contaminates the surrounding soil. The salinity level in brine is very high, sometimes approaching or exceeding that of sea water. Soils contaminated with brine are unproductive and unsuitable for plant growth. Several investigators have documented the pollution of surface water and groundwater due to brine disposal from oil and needed to clean up such sites. The objective of this study is to develop a soil washing technique that can be used to remove brine sites were collected and used in the study. This paper reports on results which indicate that soil washing using various surface active agents is effective in removing the brine

  20. Vision in hydrothermal vent shrimp.

    OpenAIRE

    Chamberlain, S C

    2000-01-01

    Bresiliid shrimp from hydrothermal vents on the Mid-Atlantic Ridge have non-imaging eyes adapted for photodetection in light environments of very low intensity. Comparison of retinal structures between both vent shrimp and surface-dwelling shrimp with imaging eyes, and between juvenile and adult vent shrimp, suggests that vent shrimp have evolved from ancestors that lived in a light environment with bright cyclic lighting. Whether the vent shrimp live in swarms and have large dorsal eyes or l...

  1. Brine Sampling and Evaluation Program

    International Nuclear Information System (INIS)

    Deal, D.E.; Case, J.B.; Deshler, R.M.; Drez, P.E.; Myers, J.; Tyburski, J.R.

    1987-12-01

    The Brine Sampling and Evaluation Program (BSEP) Phase II Report is an interim report which updates the data released in the BSEP Phase I Report. Direct measurements and observations of the brine that seeps into the WIPP repository excavations were continued through the period between August 1986 and July 1987. That data is included in Appendix A, which extends the observation period for some locations to approximately 900 days. Brine observations at 87 locations are presented in this report. Although WIPP underground workings are considered ''dry,'' small amounts of brine are present. Part of that brine migrates into the repository in response to pressure gradients at essentially isothermal conditions. The data presented in this report is a continuation of moisture content studies of the WIPP facility horizon that were initiated in 1982, as soon as underground drifts began to be excavated. Brine seepages are manifested by salt efflorescences, moist areas, and fluid accumulations in drillholes. 35 refs., 6 figs., 11 tabs

  2. Flavonóides e triterpenos de Baccharis pseudotenuifolia: bioatividade sobre Artemia salina

    Directory of Open Access Journals (Sweden)

    Moreira Francisco de Paula Madeira

    2003-01-01

    Full Text Available The phytochemical investigation of the chloroformic and methanolic extracts of the Baccharis pseudotenuifolia led to the isolation of triterpenes, steroids and flavonoids. From chloroformic extract were isolated oleanolic acid and alpha-spinasterol while from methanolic extract were isolated the flavonoids: hispidulin, naringenin, 3'-methoxy-luteolin, apigenin, kaempferol, eriodyctiol, aromadendrin, quercetin, 3'-methoxy-quercetin, quercetin-3-O-rhamnoside and quercetin-3-O-glucoside. The structure of these compounds were identified by IR, CG/MS, ¹H and 13C NMR spectral analysis and comparison with literature. In addition, the extracts were evaluated by means of Brine Shrimp Lethality test and the highier activity was observed in the chloroformic extract.

  3. Tolerance of Artemia to static and shock pressure loading

    Science.gov (United States)

    Fitzmaurice, B. C.; Appleby-Thomas, G. J.; Painter, J. D.; Ono, F.; McMillan, P. F.; Hazael, R.; Meersman, F.

    2017-10-01

    Hydrostatic and hydrodynamic pressure loading has been applied to unicellular organisms for a number of years due to interest from food technology and extremophile communities. There is also an emerging interest in the response of multicellular organisms to high pressure conditions. Artemia salina is one such organism. Previous experiments have shown a marked difference in the hatching rate of these organisms after exposure to different magnitudes of pressure, with hydrostatic tests showing hatching rates at pressures up to several GPa, compared to dynamic loading that resulted in comparatively low survival rates at lower pressure magnitudes. In order to begin to investigate the origin of this difference, the work presented here has focussed on the response of Artemia salina to (quasi) one-dimensional shock loading. Such experiments were carried out using the plate-impact technique in order to create a planar shock front. Artemia cysts were investigated in this manner along with freshly hatched larvae (nauplii). The nauplii and cysts were observed post-shock using optical microscopy to detect motility or hatching, respectively. Hatching rates of 18% were recorded at pressures reaching 1.5 GPa, as determined with the aid of numerical models. Subjecting Artemia to quasi-one-dimensional shock loading offers a way to more thoroughly explore the shock pressure ranges these organisms can survive.

  4. Effect of enriched Brachionus plicatilis and Artemia salina nauplii by ...

    African Journals Online (AJOL)

    The growth, developmental stages and survival rates of Sparus aurata larvae fed with Brachionus plicatilis and Artemia salina nauplii enriched by microalga Tetraselmis chuii were studied. Two experiments were carried out; the first concerning with culturing the microalga (T. chuii) in four different media, then using these ...

  5. Field culture of American strain of Artemia in India

    Digital Repository Service at National Institute of Oceanography (India)

    Royan, J.P.; Sumitra-Vijayaraghavan; Krishnakumari, L.

    Pilot scale culture of Artemia was carried out in a 0.22 ha condenser unit of salt pans. Pond was fertilized with inorganic salts. The nauplii of San Francisco Bay, USA strain were inoculated. Within a period of 5 months a production of 20.6 kg dry...

  6. Larvicidal, Histopathological Efficacy of Penicillium daleae against Larvae of Culex quinquefasciatus and Aedes aegypti Plus Biotoxicity on Artemia nauplii a Non-target Aquatic Organism

    Directory of Open Access Journals (Sweden)

    C. Ragavendran

    2017-10-01

    Full Text Available Mosquitoes can transmit the terrible diseases to human beings. Soil-borne fungal products act as potential source for low-cost chemicals, used for developing eco-friendly control agents against mosquito-vector borne diseases. The prime aim of study was to check the larvicidal potential of fungus mycelia (by ethyl acetate solvent extract from Penicillium daleae (KX387370 against Culex quinquefasciatus and Aedes aegypti and to test the toxicity of brine shrimp Artemia nauplii, by observing the physiological activity. The ethyl acetate extract of P. daleae mycelia (after 15 days from Potato dextrose broth (PDB medium revealed better result with least LC50 and LC90 values of I-IV instars larvae of Cx. quinquefasciatus (LC50 = 127.441, 129.087, 108.683, and 93.521; LC90 = 152.758, 158.169, 139.091, and 125.918 μg/ml and Ae. aegypti (LC50 = 105.077, 83.943, 97.158, and 76.513; LC90 = 128.035, 106.869, 125.640, and 104.606 μg/ml respectively. At higher concentration (1000 μg/ml of extracts, mortality begins at 18 h of exposure and attained 100% mortality after 48 h exposure. Overall, the activity was depends on the dose and time of exposure to the extracts. The stereomicroscopic and histopathological analysis of Ae. aegypti and Cx. quinquefasciatus larvae treated with mycelium ethyl acetate extract showed complete disintegration of abdominal region, particularly the midgut and caeca, loss of cuticular parts and caudal hairs. Morphological characterization of the fungi was performed and taxonomically identified through 5.8s rDNA technique. The phylogenetic analysis of rDNA sequence was carried out to find out the taxonomic and the evolutionary sketch of isolate in relation to earlier described genus Penicillium. Behavior and swimming speed alteration was analyzed together with mortality. The results of the experiment indicates that swimming behavior recorder (SBR is a appropriate tool to detect individual swimming speed of the A. nauplii organisms

  7. Gulf Shrimp System

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Gulf of Mexico Shrimp Landings - This data set contains catch (landed catch) and effort for fishing trips made by the larger vessels that fish near and offshore for...

  8. Development of shrimp in small ponds

    Directory of Open Access Journals (Sweden)

    Armando Adolfo Ortega Salas

    2014-06-01

    Full Text Available Development of white shrimp Litopenaeus vannamei in small ponds ( 6 m3 in fresh water (2-3‰ and seawater; ponds 3.66 x 1.65 x 1.0 m; availability of fresh water, sea water, aeration and drainage. Two cycles of three months each were made. The postlarvae were acclimated to seawater fresh water in four days. Four hundred postlarvas/m3 were seeded in freshwater pond and 500 in the pool of seawater. First, a culture of Daphnia magna in the freshwater pond, also appeared chyronomid larvae; Artemia cysts were seeded in sea water as a dietary supplement. The shrimp were fed Camaronina (25% protein at libitum, daily; is offered on a tray of food; the temperature ranged between 27 and 30° C, oxygen 4.26 ± 1.43 mg / L , pH between 7 and 8 . Detritus siphoned every third day. Water changes between 10 and 20% are often performed. The feed conversion rate (FCR was 1:1.3 . The shrimp were measured in length and weight to calculate weekly growth by Bertalanffy model. Survival in the first cycle was 95.8 , and 97.9% for the second cycle. In seawater parameters of the population of the first cycle were k = 0.0301, L ∞ = 322.16 and t0 = -0.8852, the second cycle of k = 0.0203, L ∞ = 294.42 and t0 = -5.3771. The biomass of 27 kg was obtained for the first cycle and 16 kg for the second cycle. Freshwater population parameters of the first cycle were k = 0.0957, L ∞ = 146.98 and t0 = - 0.93; in the second cycle of k = 0.0172 , L ∞ = 367.82 and t0 = - 4.60. The biomass of 26 kg was obtained for the first cycle and 16 kg for the second cycle. The results indicate a rapid growth during the first 10 weeks. In small ponds can be handled well aseptic conditions without disease problems, good crop was obtained.

  9. Use of the Crustacean Artemia franciscana for Alternative Biotests

    Directory of Open Access Journals (Sweden)

    Petr Dvořák

    2010-01-01

    Full Text Available Use of the crustacean Artemia franciscana for alternative biotests of the second generation was studied, and possible experimental design and applications of such tests outlined. In addition to the classical use in ecotoxicology, the test can be used in pharmacology as well, or to monitor the effects of ionizing radiation in co-exposure with some chemical compounds. The synergistic effect of co-exposure of PCB (DELOR 103, cadmium chloride and potassium dichromate with beta 89Sr irradiation was shown. We also demonstrated the anti-oxidative and pro-oxidative effects of the ascorbic acid in dependence on its concentration. Use of the pharmaco-toxicological screening in search for the novel inhibitors of cyclin-dependent kinases was demonstrated as well, showing that Artemia franciscana may be used as a suitable biosensor instead of the expensive tests on higher vertebrates.

  10. Burrowing behavior of penaeid shrimps

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Brown shrimp, Farfantepenaeus aztecus, and white shrimp, Litopenaeus setiferus, were held were held under natural light conditions before experiments. Experiments...

  11. Stimulating effect of space flight factors on Artemia cysts: comparison with irradiation by gamma rays

    International Nuclear Information System (INIS)

    Gaubin, Y.; Pianezzi, B.; Gasset, G.; Plannel, H.; Kovalev, E.E.

    1986-01-01

    The Artemia cyst, a gastrula in dormant state, is a very suitable material to investigate the individual effects of HZE cosmic particles. Monolayers of Artemia cysts, sandwiched with nuclear emulsions, flew aboard the Soviet biosatellite Cosmos 1129. The space flight stimulated the developmental capacity expressed by higher percentages of emergence, hatching, and alive nauplii at day 4-5. A greater mean life span was reported in Artemias developed from Artemia cysts hit by the cosmic heavy ions. On Earth, Artemia cysts were exposed to 1, 10, 100, 200 and 400 Gy of gamma (gamma) rays. A stimulating effect on developmental capacity was observed for 10 Gy; the mean life span was significantly increased for this dose. These results are discussed in comparison with previous investigations performed on Earth and in space

  12. Distribution and abundance of Artemia salina in the Salt Lake Basin (Central Anatolia, Turkey

    Directory of Open Access Journals (Sweden)

    Alaş Ali

    2017-06-01

    Full Text Available In this study, the distribution and abundance of Artemia salina in 10 different stations of the Salt Lake basin were investigated. In addition, its relationship to pH, dissolved oxygen, temperature, electrical conductivity and water levels were analyzed. Field studies were carried out from July to August of 2010. Artemia salina was observed in five of these stations. Artemia salina was not seen in some stations that have high electrical conductivity. It is determined that, in the station named Tersakan Lake where electrical conductivity was 154 mS/cm, Artemia salina is more abundant when compared to the other stations. But as underground water pumps that are built for the irrigation of agricultural lands decrease water levels, Artemia salina’s life is under threat.

  13. Survival and growth of catfish Pangasionodon sp. larvae fed on vitamin C-enriched Artemia

    Directory of Open Access Journals (Sweden)

    Mia Setiawati

    2015-05-01

    Full Text Available ABSTRACT The research was conducted to evaluate effect of the administration of Artemia enriched with vitamin C on the survival, total body length, and average body weight of Pangasionodon sp. larvae. Artemia were enriched with either 0, 50, 100, or 150 mg/L vitamin C immersed in the culture medium for 13 hours. Two days old larvae were fed on enriched Artemia 12 times/day for seven days. At the end of feeding experiment, it was found that fish fed on Artemia enriched with 100 mg/L culture medium had the highest survival, total length and body weight, respectively 76.17±12.78%, 1.63±0.34 cm, and 20.6±3.8 mg. Keywords: survival, growth, Artemia, vitamin C, catfish, Pangasionodon sp.  ABSTRAK Penelitian ini dilakukan untuk mengetahui pengaruh Artemia yang diperkaya dengan vitamin C dosis berbeda terhadap kelangsungan hidup, panjang total, dan bobot tubuh larva ikan patin Pangasionodon sp. Pada penelitian ini, Artemia diperkaya dengan vitamin C dosis 0, 50, 100, atau 150 mg/L media pengayaan. Larva yang berumur dua hari diberi pakan Artemia hasil pengayaan sebanyak 12 kali sehari selama tujuh hari masa pemeliharaan. Pada akhir percobaan, didapatkan bahwa larva ikan yang diberi Artemia diperkaya vitamin C dosis 100 mg/L media pengayaan memberikan kelangsungan hidup 76,17±12,78%, panjang total 1,63±0,34 cm, dan bobot tubuh paling tinggi 20,6±3,8 mg. Kata kunci: kelangsungan hidup, pertumbuhan, Artemia, vitamin C, ikan patin, Pangasionodon sp. 

  14. Solar Pond devices: free energy or bioreactors for Artemia biomass production?

    Science.gov (United States)

    Gouveia, Luisa; Sousa, João; Marques, Ana; Tavares, Célia; Giestas, Margarida

    2009-08-01

    The recent exponential growth in industrial aquaculture has led to a huge increase in Artemia biomass production in order to meet increased fish production needs. The present study explores the potential use of salt gradient solar ponds (SGSPs) for production of Artemia nauplii. An SGSP is a basin of water where solar energy is trapped and collected via an artificially imposed gradient. Three zones can be identified in an SGSP: upper and lower zones, which are both convective, and a middle zone, which is intended to be non-convective. The latter acts as a transparent insulation layer and allows for storage of solar energy at the bottom, where it is available for use. The combination of salt, temperature and high transparency could make SGSPs promising bioreactors for the production of Artemia nauplii. Using particle image velocymetry (PIV) and Shadowgraph visualisation techniques, the behaviour of Artemia nauplii under critical cultivation parameters (namely, salinity, temperature and light) was monitored to determine movement velocity, and how movement of Artemia affects the salt gradient. It was observed that Artemia nauplii constantly follow light, irrespective of adverse salinity and/or temperature conditions. However, despite the substantial displacement of Artemia following the light source, the salt gradient is not disrupted. The suitability of SGSPs as bioreactors for Artemia biomass production was then tested. The results were disappointing, probably due to the lack of sufficient O(2) for Artemia survival and growth. Follow-up trials were conducted aimed at using the SGSP as a green and economically attractive energy source to induce faster hatching of cysts and improved Artemia nauplii growth. The results of these trials, and a case study of Artemia nauplii production using an SGSP, are presented. The authors constructed a Solar Pond device, which they suggest as a novel way of supplying thermal energy for Artemia biomass production in an aquaculture

  15. Brine Sampling and Evaluation Program, 1991 report

    Energy Technology Data Exchange (ETDEWEB)

    Deal, D.E.; Abitz, R.J.; Myers, J.; Martin, M.L.; Milligan, D.J.; Sobocinski, R.W.; Lipponer, P.P.J. [International Technology Corp., Albuquerque, NM (United States); Belski, D.S. [Westinghouse Electric Corp., Carlsbad, NM (United States). Waste Isolation Div.

    1993-09-01

    The data presented in this report are the result of Brine Sampling and Evaluation Program (BSEP) activities at the Waste Isolation Pilot Plan (WIPP) during 1991. These BSEP activities document and investigate the origins, hydraulic characteristics, extent, and composition of brine occurrences in the Permian Salado Formation and seepage of that brine into the excavations at the WIPP. When excavations began at the WIPP in 1982, small brine seepages (weeps) were observed on the walls. Brine studies began as part of the Site Validation Program and were formalized as a program in its own right in 1985. During nine years of observations (1982--1991), evidence has mounted that the amount of brine seeping into the WIPP excavations is limited, local, and only a small fraction of that required to produce hydrogen gas by corroding the metal in the waste drums and waste inventory. The data through 1990 is discussed in detail and summarized by Deal and others (1991). The data presented in this report describes progress made during the calendar year 1991 and focuses on four major areas: (1) quantification of the amount of brine seeping across vertical surfaces in the WIPP excavations (brine ``weeps); (2) monitoring of brine inflow, e.g., measuring brines recovered from holes drilled downward from the underground drifts (downholes), upward from the underground drifts (upholes), and from subhorizontal holes; (3) further characterization of brine geochemistry; and (4) preliminary quantification of the amount of brine that might be released by squeezing the underconsolidated clays present in the Salado Formation.

  16. Possible Mars brines - Equilibrium and kinetic considerations

    Science.gov (United States)

    Zent, A. P.; Fanale, F. P.

    1986-01-01

    To determine the fate of postulated near surface brines on Mars, the rate of H2O mass loss from subsurface brines was calculated as a function of latitude, depth, regolith porosity, eutectic temperature, and pore size. A model for a chemically reasonable brine that could reproduce Martian radar results was developed, and the escape rate of H2O molecules from such a brine was estimated. It is suggested that the presence of a low-permeability duricrust may be required to preserve such a brine for reasonable periods, and to prevent detection of an extensive subsurface system by the Viking MAWD instrument.

  17. Growth and survival of Hippocampus erectus (Perry, 1810 juveniles fed on Artemia with different HUFA levels

    Directory of Open Access Journals (Sweden)

    Nicolás Vite-Garcia

    2014-03-01

    Full Text Available Survival during first months after birth is one of the bottlenecks for consolidating the seahorse farming industry. In this work, Artemia metanauplii enriched with two highly unsaturated fatty acids (HUFA rich commercial emulsions with different docosahexaenoic acid (DHA levels (63% and 14% of total lipids, a vegetable oil with no DHA, and non-enriched Artemia as control, were used to feed 5-day-old juvenile Hippocampus erectus for 60 days. Enriched Artemia had similar levels of DHA (13% and 9%, despite great differences of DHA in the emulsions, with traces of DHA in non-enriched and vegetable oil enriched Artemia. More than 20% of DHA was found in 24 h starved juveniles fed both DHA-enriched treatments, similar to values in newly born juveniles, but those fed vegetable oil enriched Artemia or non-enriched Artemia had 5% of DHA. Total lipid and protein levels were similar in juveniles from the four treatments. The n-3/n-6 ratio was almost four-fold higher in seahorses fed DHA-enriched treatments compared to juveniles fed the non-enriched treatments. Survival of seahorses only partially reflected the DHA levels: it was lower in the vegetable oil treatment, similar in the seahorses fed Artemia with higher DHA and in the control treatment, and higher in seahorses fed the HUFA-enriched Artemia with lower DHA levels, although growth was similar in the two DHA-enriched Artemia treatments. Juvenile H. erectus seahorses perform better when they have at least 20% of DHA in their tissues, and these levels can be attained with no more than 14% of DHA in emulsions, eliminating the need for more expensive emulsions with higher DHA levels.

  18. Brine Sampling and Evaluation Program, 1990 report

    International Nuclear Information System (INIS)

    Deal, D.E.; Abitz, R.J.; Myers, J.; Case, J.B.; Martin, M.L.; Roggenthen, W.M.; Belski, D.S.

    1991-08-01

    The data presented in this report are the result of Brine Sampling and Evaluation Program (BSEP) activities at the Waste Isolation Pilot Plant (WIPP) during 1990. When excavations began in 1982, small brine seepages (weeps) were observed on the walls. These brine occurrences were initially described as part of the Site Validation Program. Brine studies were formalized in 1985. The BSEP activities document and investigate the origins, hydraulic characteristics, extent, and composition of brine occurrences in the Permian Salado Formation and seepage of that brine into the excavations at the WIPP. The brine chemistry is important because it assists in understanding the origin of the brine and because it may affect possible chemical reactions in the buried waste after sealing the repository. The volume of brine and the hydrologic system that drives the brine seepage also need to be understood to assess the long-term performance of the repository. After more than eight years of observations (1982--1990), no credible evidence exists to indicate that enough naturally occurring brine will seep into the WIPP excavations to be of practical concern. The detailed observations and analyses summarized herein and in previous BSEP reports confirm the evidence apparent during casual visits to the underground workings -- that the excavations are remarkably dry

  19. Brine Sampling and Evaluation Program, 1990 report

    Energy Technology Data Exchange (ETDEWEB)

    Deal, D.E.; Abitz, R.J.; Myers, J.; Case, J.B.; Martin, M.L.; Roggenthen, W.M. [International Technology Corp., Albuquerque, NM (United States); Belski, D.S. [Westinghouse Electric Corp., Carlsbad, NM (United States). Waste Isolation Div.

    1991-08-01

    The data presented in this report are the result of Brine Sampling and Evaluation Program (BSEP) activities at the Waste Isolation Pilot Plant (WIPP) during 1990. When excavations began in 1982, small brine seepages (weeps) were observed on the walls. These brine occurrences were initially described as part of the Site Validation Program. Brine studies were formalized in 1985. The BSEP activities document and investigate the origins, hydraulic characteristics, extent, and composition of brine occurrences in the Permian Salado Formation and seepage of that brine into the excavations at the WIPP. The brine chemistry is important because it assists in understanding the origin of the brine and because it may affect possible chemical reactions in the buried waste after sealing the repository. The volume of brine and the hydrologic system that drives the brine seepage also need to be understood to assess the long-term performance of the repository. After more than eight years of observations (1982--1990), no credible evidence exists to indicate that enough naturally occurring brine will seep into the WIPP excavations to be of practical concern. The detailed observations and analyses summarized herein and in previous BSEP reports confirm the evidence apparent during casual visits to the underground workings -- that the excavations are remarkably dry.

  20. Radurization of brown shrimps

    International Nuclear Information System (INIS)

    Ehlermann, D.; Muenzner, R.

    1976-01-01

    Brown shrimps (Crangon vulgaris) from the North sea coast were blanched on board, and irradiated with cobalt-60 gamma-rays at a minimum dose of 130 krad either before or after peeling. Other samples were irradiated before and after peeling. Control samples remained untreated or were preserved with benzoic acid. Irradiation before peeling did not result in a lasting improvement of keeping quality. However, irradiation of the peeled shrimp meat resulted in a reduction of the total bacterial load by up to 4 orders of magnitude. Shelf life until the initial microbial count was reached was 9 days for chemically treated samples, 18 days for samples irradiated after peeling, and 20 days for samples irradiated before and after peeling. Sensory evaluation and determination of volatile basic nitrogen gave similar results. The obtained diminution of the counts of staphylococci, enterococci and enterobacteriaceae reduces the hygienic hazard. Radurization of shrimp meat proved to be a satisfactory means of preservation. Introduction of the product into the market and best presentation and packaging require further investigations. (orig.) [de

  1. Brine Sampling and Evaluation Program: 1988 report

    International Nuclear Information System (INIS)

    Deal, D.E.; Abitz, R.J.; Case, J.B.; Crawley, M.E.; Deshler, R.M.; Drez, P.E.; Givens, C.A.; King, R.B.; Myers, J.; Pietz, J.M.; Roggenthen, W.M.; Tyburski, J.R.; Belski, D.S.; Niou, S.; Wallace, M.G.

    1989-12-01

    The data presented in this report are the result of Brine Sampling and Evaluation Program (BSEP) activities at the Waste Isolation Pilot Plant (WIPP) during 1988. These activities, which are a continuation and update of studies that began in 1982 as part of the Site Validation Program, were formalized as the BSEP in 1985 to document and investigate the origins, hydraulic characteristics, extent, and composition of brine occurrences in the Permian Salado Formation, and seepage of that brine into the excavations at the WIPP. Previous BSEP reports (Deal and Case, 1987; Deal and others, 1987) described the results of ongoing activities that monitor brine inflow into boreholes in the facility, moisture content of the Salado Formation, brine geochemistry, and brine weeps and crusts. The information provided in this report updates past work and describes progress made during the calendar year 1988. During 1988, BSEP activities focused on four major areas to describe and quantify brine activity: (1) monitoring of brine inflow parameters, e.g., measuring brines recovered from holes drilled upward from the underground drifts (upholes), downward from the underground drifts (downholes), and near-horizontal holes; (2) characterizing the brine, e.g., the geochemistry of the brine and the presence of bacteria and their possible interactions with experiments and operations; (3) characterizing formation properties associated with the occurrence of brine; e.g., determining the water content of various geologic units, examining these units in boreholes using a video camera system, and measuring their resistivity (conductivity); and (4) modeling to examine the interaction of salt deformation near the workings and brine seepage through the deforming salt. 77 refs., 48 figs., 32 tabs

  2. Nutritional aspects of irradiated shrimp

    International Nuclear Information System (INIS)

    Shamsuzzaman, K.

    1989-11-01

    Data available in the literature on the nutritional aspects of irradiated shrimp are reviewed and the indication is that irradiation of shrimp at doses up to about 3.2 kGy does not significantly affect the levels of its protein, fat, carbohydrate and ash. There are no reports on the effect of irradiation of shrimp above 3.2 kGy on these components. Limited information available indicates that there are some minor changes in the fatty acid composition of shrimp as a result of irradiation. Irradiation also causes some changes in the amino acid composition of shrimp; similar changes occur due to canning and hot-air drying. Some of the vitamins in shrimp, such as thiamine, are lost as a result of irradiation but the loss is less extensive than in thermally processed shrimp. Protein quality of shrimp, based on the growth of rats as well as that of Tetrahymena pyriformis, is not affected by irradiation. No adverse effects attributed to irradiation were found either in short-term or long-term animal feeding tests

  3. Origin of the yellow brine and the black brine in Sichuan Basin

    International Nuclear Information System (INIS)

    Wang Dongsheng

    1988-01-01

    The spring water, geothermal water and Cretaceous brine in the outer zone of the Sichuan Basin has the Craig relationship, and they are cycling waters. The brine in the inner zone is mainly metasedimentary water. A basic feature of them is poor in 2 H, but rich in 18 O. The δD-values of the yellow brine in Jurassic and Upper Triassic aquifer of continental facies varies from -62.25 to -22.4, and the δ 18 O-values are -6.72 - +6.02. The δD-values of the black brine in marine aquifer (T 2 ,T 1 ,P,C,O and so on) varies from -49 to -25.1, and the 18 O values are +3.89 - +6.14. The δD of yellow brine is similar to that of meteoric water, and the δD of the black brine is around that of crystallization water expelled from gypsum by anhydritization. Increases of salinity in Jurassic yellow brine result primarily from the evapotranspiration process. The salinity in Upper Triassic yellow brine in Aa sub-area originated from underlying rock salt which was leached by paleometeoric water. Triassic black brine derived from the mixing of the crystallization water leached from rock salt with the residual sea water after salt crystallization. In Zhigong, the composition of yellow brine has mainly been changed by the mixing of the yellow brine with the black brine. (author). 2 refs, 2 figs, 2 tabs

  4. REFUSE OF FERMENTATION BRINES IN THE CUCUMBER PICKLING INDUSTRY

    Science.gov (United States)

    The project evaluated on a commercial scale the technological and economic feasibility of recycling spent cucumber fermentation brine. Two brine treatment procedures, heat treatment and chemical treatment, were used. The results showed that brine recycling was practical on a comm...

  5. On the physico-chemical characteristics of brines

    Digital Repository Service at National Institute of Oceanography (India)

    Shirodkar, P.V.; Rao, P.V.S.S.D.P.; Singbal, S.Y.S.

    Analyses of the natural brines form the salt lakes, salt pans and the artificial brines obtained after the solar desalination of seawater respectively, showed wide differences in their physico-chemical characteristics. The natural brines are markEd...

  6. Solubility of Nd in brine

    International Nuclear Information System (INIS)

    Khalili, F.I.; Symeopoulos, V.; Chen, J.F.; Choppin, G.R.

    1994-01-01

    The solubility of Nd(III) has been measured at 23±3 C in a synthetic brine at pcH 6.4, 8.4, 10.4 and 12.4. The brine consisted predominantly of (Na+K)Cl and MgCl 2 with an ionic strength of 7.8 M (9.4 m) a solid compound of Nd(III) at each pcH was assigned from X-ray diffraction patterns. The log values of the experimental solubilities decrease fomr -3 at pcH 6.4 to -5.8 at pcH 8.4; at pcH 10.4 and 12.4 the solubility was below the detection limit of -7.5. The experimental solubility does not follow closely the variation with pcH estimated from modeling of the species in solution in equilibrium with the Nd solid using S.I.T. (orig.)

  7. Observations on development of artemia eggs irradiated on earth

    International Nuclear Information System (INIS)

    He Jian; Zhou Qiling; Xing Guoren; Zheng Decun; Su Ruizhen; Chen Qu'e

    1990-01-01

    For accompanying with space radiobiological studies, dry eggs of native Artemia were irradiated in the laboratory by 14 MeV fast neutrons and 60 Co γ-rays, and their development was then followed. Doses of the fast neutrons used were 10, 50, 100, 200, 400, 600, 800 and 1000 Gy, and those of γ-rays were 50, 100, 500, 1000 and 2000 Gy respectively. The irradiated eggs, together with the control, were stored at 4 deg C before incubation and observations. The results are as follows: (1) A significant delay of early development was seen in eggs irradiated by both radiatoins. (2) The rates of emergence and hatching decreased with the increase of the doses, and went down further after the prolongation of egg-storage at 4 deg C. (3) The mortality peak of the larvae hatched from irradiated eggs appeared within 2-5 days after hatching, and the RBE's of the fast neutrons, for LD 50 , comparing with the γ-rays were found to be: 1.80 on the 3rd day, 2.44 on the 4th day, and 2.27 on the 5th day

  8. Reducing Vibrio load in Artemia nauplii using antimicrobial photodynamic therapy: a promising strategy to reduce antibiotic application in shrimp larviculture

    Digital Repository Service at National Institute of Oceanography (India)

    Aparna, A.; Arshad, E.; Jasmin, C.; Pai, S.S.; BrightSingh, I.S.; Mohandas, A.; Anas, A.

    by treating the cells with Rose Bengal and photosensitizing for 30 min using a halogen lamp. This resulted in the death of more than 50% of the cells within the first 10 min of exposure and the 50% reduction in the cell wall integrity after 30 min could...

  9. Migration of brine inclusions in salt

    International Nuclear Information System (INIS)

    Pigford, T.H.

    1982-01-01

    Theories of the migration of brine inclusions in salt are interpreted as simple physical processes, and theories by Russian and U.S. workers are shown to yield the same results. The migration theory is used to predict threshold temperature gradients below which migration of brine inclusions should not occur. The predicted threshold gradients are compared with the temperature gradients expected at the Waste Isolation Pilot Plant in New Mexico. The theory of a threshold gradient helps explain the existence of brine inclusions in natural salt deposits

  10. Zooplankton at deep Red Sea brine pools

    KAUST Repository

    Kaartvedt, Stein

    2016-03-02

    The deep-sea anoxic brines of the Red Sea comprise unique, complex and extreme habitats. These environments are too harsh for metazoans, while the brine–seawater interface harbors dense microbial populations. We investigated the adjacent pelagic fauna at two brine pools using net tows, video records from a remotely operated vehicle and submerged echosounders. Waters just above the brine pool of Atlantis II Deep (2000 m depth) appeared depleted of macrofauna. In contrast, the fauna appeared to be enriched at the Kebrit Deep brine–seawater interface (1466 m).

  11. Waste glass/metal interactions in brines

    International Nuclear Information System (INIS)

    Shade, J.W.; Pederson, L.R.; McVay, G.L.

    1983-05-01

    Leaching studies of MCC 76-68 glass in synthetic brines high in NaCl were performed from 50 to 150 0 C and included interactive testing with ductile iron and titanium. Hydrolysis of the glass matrix was generally slower in saturated brines than in deionized water, due to a lower solubility of silica in the brines. Inclusion of ductile iron in the tests resulted in accelerated leach rates because irion-silica reactions occurred which reduced the silica saturation fraction. At 150 0 C, iron also accelerated the rate of crystalline reaction product formation which were primarily Fe-bearing sepiolite and talc. 16 references

  12. Role of women in Indian shrimp farming

    OpenAIRE

    Gopalakrishnan, A.

    1996-01-01

    Women in India are involved in various facets of shrimp (Penaeus monodon; Penaeus indicus) farming, including pond construction, seed collection, collection of feed materials and preparation of feeds, pond maintenance and post-harvest handling. This study indicates that 40% of laborers involved in shrimp farm construction are women. The various roles of women in shrimp farming are also described.

  13. Experimental studies on the larval development of the shrimps Crangon crangon and C. allmanni

    Science.gov (United States)

    Criales, M. M.; Anger, K.

    1986-09-01

    Larvae of the shrimps Crangon crangon L. and C. allmanni Kinahan were reared in the laboratory from hatching through metamorphosis. Effects of rearing methods (larval density, application of streptomycin, food) and of salinity on larval development were tested only in C. crangon, influence of temperature was studied in both species. Best results were obtained when larvae were reared individually, with a mixture of Artemia sp. and the rotifer Brachionus plicatilis as food. Streptomycin had partly negative effects and was thus not adopted for standard rearing techniques. All factors tested in this study influenced not only the rates of larval survival and moulting, but also morphogenesis. In both species, in particular in C. crangon, a high degree of variability in larval morphology and in developmental pathways was observed. Unsuitable conditions, e.g. crowding in mass culture, application of antibiotics, unsuitable food (rotifers, phytoplankton), extreme temperatures and salinities, tend to increase the number of larval instars and of morphological forms. The frequency of moulting is controlled mainly by temperature. Regression equations describing the relations between the durations of larval instars and temperature are given for both Crangon species. The number of moults is a linear function of larval age and a power function of temperature. There is high variation in growth (measured as carapace length), moulting frequency, morphogenesis, and survival among hatches originating from different females. The interrelations between these different measures of larval development in shrimps and prawns are discussed.

  14. Enhanced Brine Dewatering System, Phase I

    Data.gov (United States)

    National Aeronautics and Space Administration — The purpose of the Enhanced Brine Dewatering System (EBDS) is to provide an easily scalable means of completely recovering usable water from byproducts created by...

  15. Brine Dewatering Using Ultrasonic Nebulization, Phase I

    Data.gov (United States)

    National Aeronautics and Space Administration — Recovery of water from brine is critically important for manned space exploration. Resupply of water is prohibitively costly for extended missions. It is anticipated...

  16. Ice Control with Brine on Highways

    DEFF Research Database (Denmark)

    Bolet, Lars

    traffic flow the spread rate of pure sodium chloride (and thus the environmental impact) in the pre-salting operations was cut back by more than one third. Compared to neighbouring counties the use of salt is less than fifty percent per square meter. In addition, supply of brine from two mixer...... of interpreting this information. The improvements gained by the county of Funen were mainly due to the use of technologies (brine spreading with nozzles) giving a more precise spread pattern than the traditional gritting of pre-wetted salt. Major challenges in the process have been to verify the higher quality...... of the nozzles spread pattern, to ensure maximum utilization of volume of brine carried by the spreading vehicles and to control the mixing of brine without getting stratification in the mixture. Moreover, of course, to ensure political approval of abandoning a well-served technology and to organize...

  17. Brine Dewatering Using Ultrasonic Nebulization, Phase II

    Data.gov (United States)

    National Aeronautics and Space Administration — Recovery of water from brine is critically important for future manned space exploration. Resupply of water is prohibitively costly for such extended missions. Water...

  18. Distillation Brine Purification for Resource Recovery Applications

    Data.gov (United States)

    National Aeronautics and Space Administration — Wastewater processing systems for space generate residual brine that contains water and salts that could be recovered to reduce life support consumables. The project...

  19. Enhanced Brine Dewatering System, Phase II

    Data.gov (United States)

    National Aeronautics and Space Administration — The purpose of the Enhanced Brine Dewatering System (EBDS) is to provide a scalable means of completely recovering usable water from byproducts created by reverse...

  20. Jarosite dissolution rates in perchlorate brine

    Science.gov (United States)

    Legett, Carey; Pritchett, Brittany N.; Elwood Madden, Andrew S.; Phillips-Lander, Charity M.; Elwood Madden, Megan E.

    2018-02-01

    Perchlorate salts and the ferric sulfate mineral jarosite have been detected at multiple locations on Mars by both landed instruments and orbiting spectrometers. Many perchlorate brines have eutectic temperatures bearing rocks and sediments may have been altered by perchlorate brines. Here we measured jarosite dissolution rates in 2 M sodium perchlorate brine as well as dilute water at 298 K to determine the effects of perchlorate anions on jarosite dissolution rates and potential reaction products. We developed a simple method for determining aqueous iron concentrations in high salinity perchlorate solutions using ultraviolet-visible spectrophotometry that eliminates the risk of rapid oxidation reactions during analyses. Jarosite dissolution rates in 2 M perchlorate brine determined by iron release rate (2.87 × 10-12 ±0.85 × 10-12 mol m-2 s-1) were slightly slower than the jarosite dissolution rate measured in ultrapure (18.2 MΩ cm-1) water (5.06 × 10-12 mol m-2 s-1) using identical methods. No additional secondary phases were observed in XRD analyses of the reaction products. The observed decrease in dissolution rate may be due to lower activity of water (ɑH2O = 0.9) in the 2 M NaClO4 brine compared with ultrapure water (ɑH2O = 1). This suggests that the perchlorate anion does not facilitate iron release, unlike chloride anions which accelerated Fe release rates in previously reported jarosite and hematite dissolution experiments. Since dissolution rates are slower in perchlorate-rich solutions, jarosite is expected to persist longer in perchlorate brines than in dilute waters or chloride-rich brines. Therefore, if perchlorate brines dominate aqueous fluids on the surface of Mars, jarosite may remain preserved over extended periods of time, despite active aqueous processes.

  1. Space and Industrial Brine Drying Technologies

    Science.gov (United States)

    Jones, Harry W.; Wisniewski, Richard S.; Flynn, Michael; Shaw, Hali

    2014-01-01

    This survey describes brine drying technologies that have been developed for use in space and industry. NASA has long considered developing a brine drying system for the International Space Station (ISS). Possible processes include conduction drying in many forms, spray drying, distillation, freezing and freeze drying, membrane filtration, and electrical processes. Commercial processes use similar technologies. Some proposed space systems combine several approaches. The current most promising candidates for use on the ISS use either conduction drying with membrane filtration or spray drying.

  2. Optimised selenium enrichment of Artemia sp. feed to improve red drum (Sciaenops ocellatus) larvae rearing.

    Science.gov (United States)

    Juhász, Péter; Lengyel, Szvetlana; Udvari, Zsolt; Sándor, Alex Nagy; Stündl, László

    2017-09-01

    Selenium is an essential microelement for the normal functioning of life processes. Moreover, it is a component of enzymes with antioxidant effects. However, it has the smallest window of any micronutrient between requirement and toxicity. Selenium is a regularly used element in fish feeds; moreover, enriching zooplankton with selenium to rear larvae is also a well-known technology. It is accepted that the most common starter foods of fish larvae, natural rotifers contain the smallest dosage of selenium, but providing selenium enriched Artemia sp. instead could increase survival and growth rate of fish. However, no such references are available for the red drum (Sciaenops ocellatus) larvae. Therefore, in this study, Artemia sp. was enriched with nano-selenium of verified low toxicity and easy availability in 5 treatments (1, 5, 10, 50, 100 mg/l Se), and then, fish larvae were fed with four of these enriched Artemia stocks (1, 5, 10, 50 mg/l Se) and a control group. At the end of the 9-day-long experiment, survival rate (S) and growth parameters (SL, W, K-factor, SGR) of fish larvae were calculated as well as their selenium retention and glutathione peroxidase enzyme activity were analysed. It was revealed that a moderate level of selenium enrichment (~4 mg/kg dry matter) of Artemia sp. positively influences the rearing efficiency (i.e. survival and growth) of fish larvae, but higher dosages of selenium could cause adverse effects.

  3. Yaequinolones, new insecticidal antibiotics produced by Penicillium sp. FKI-2140. I. Taxonomy, fermentation, isolation and biological activity.

    Science.gov (United States)

    Uchida, Ryuji; Imasato, Rie; Yamaguchi, Yuichi; Masuma, Rokuro; Shiomi, Kazuro; Tomoda, Hiroshi; Omura, Satoshi

    2006-10-01

    New nine insecticidal antibiotics designated yaequinolones were isolated from the culture broth of the fungal strain Penicillium sp. FKI-2140 by solvent extraction, centrifugal partition chromatography and HPLC. Yaequinolones showed growth inhibitory activity against brine shrimp (Artemia salina). Among them, yaequinolone F has the most potent activity with MIC value of 0.19 microg/ml.

  4. Journal of Biosciences | Indian Academy of Sciences

    Indian Academy of Sciences (India)

    2004-10-27

    Oct 27, 2004 ... Encysted embryos (cysts) of the brine shrimp, Artemia, provide excellent opportunities for the study of biochemical and biophysical adaptation to extremes of environmental stress in animals. Among other virtues, this organism is found in a wide variety of hypersaline habitats, ranging from deserts, to tropics, ...

  5. Journal of Biosciences | Indian Academy of Sciences

    Indian Academy of Sciences (India)

    Clipboard: Order in stress – Lessons from the inanimate world .... indica A. Juss and their role in a multicomponent system against lepidopteran larvae .... Heat stress response in plants: a complex game with chaperones and more than twenty heat ... Encysted embryos (cysts) of the brine shrimp, Artemia, provide excellent ...

  6. Brine flow in heated geologic salt.

    Energy Technology Data Exchange (ETDEWEB)

    Kuhlman, Kristopher L.; Malama, Bwalya

    2013-03-01

    This report is a summary of the physical processes, primary governing equations, solution approaches, and historic testing related to brine migration in geologic salt. Although most information presented in this report is not new, we synthesize a large amount of material scattered across dozens of laboratory reports, journal papers, conference proceedings, and textbooks. We present a mathematical description of the governing brine flow mechanisms in geologic salt. We outline the general coupled thermal, multi-phase hydrologic, and mechanical processes. We derive these processes governing equations, which can be used to predict brine flow. These equations are valid under a wide variety of conditions applicable to radioactive waste disposal in rooms and boreholes excavated into geologic salt.

  7. Comparison of bioassays using the anostracan crustaceans Artemia salina and Thamnocephalus platyurus for plant extract toxicity screening

    OpenAIRE

    Mayorga,Pablo; Pérez,Karen R.; Cruz,Sully M.; Cáceres,Armando

    2010-01-01

    Three lethality bioassays, using the salt-water crustacean Artemia salina Leach, Artemiidae, (conventional 96 microwell plate test and the Artoxkit M microbiotest) and the freshwater crustacean Thamnocephalus platyurus Packard, Thamnocephalidae, (Thamnotoxkit F microbiotest), were compared using extracts of ten Guatemalan plant species. It was previously observed that five of them have anti-Artemia activity. These were: Solanum americanum Mill., Solanaceae, Gliricidia sepium (Jacq.) Kunth ex ...

  8. Antioxidant, Antibacterial activity and Brine shrimp toxicity test of some Mountainous Lichens from Nepal

    Directory of Open Access Journals (Sweden)

    Babita Paudel

    2012-01-01

    Full Text Available A total of twenty four lichen species belonging to six families were collected from mountainous region of Nepal. The methanol extracts of each species were tested for antimicrobial and antioxidant activitiesin vitro. It was found that extracts of twenty one lichen species were active againstB. subtilis and seven species were active againstS. aureus. Similarly, in DPPH assay, three speciesPeltigera sp.,Cladonia sp., andCanoparmelia sp. showed comparable activity with commercial standard, BHA. In ABTS+ assay, extracts ofParmoterma sp.,Ramalina sp.,Peltigera sp. andCladonia sp. showed stronger activity than ascorbic acid. The observed data after comparison with previously published reports indicated that the high altitude lichens contain stronger antioxidant and antibacterial constituents. Similarly, the methanol extracts ofHeterodermia sp. andRamalina sp. showed comparable toxicity effect with commercial standard berberine chloride indicating a potent source of anticancer drugs.

  9. Toxicity of Nanoparticles to Brine Shrimp: An Introduction to Nanotoxicity and Interdisciplinary Science

    Science.gov (United States)

    Maurer-Jones, Melissa A.; Love, Sara A.; Meierhofer, Sharon; Marquis, Bryce J.; Liu, Zhen; Haynes, Christy L.

    2013-01-01

    Nanotoxicity is an area of intense research, stimulated by increased use of nanoparticles in commercially available products. Herein, using nanotoxicity as a platform, we describe an experiment that emphasizes interdisciplinary science in a collaborative work setting while expanding the traditional realm of chemistry and chemistry research.…

  10. Uji Toksisitas Ekstrak Tinta Cumi-Cumi (Photololigo Duvaucelii) Dengan Metode Brine Shrimp Lethality Test (Bslt)

    OpenAIRE

    Kurniansyah, Wendra

    2015-01-01

    During this people considers that squid ink is not useful so that if processing squid, the ink was removed. Based on research conducted by Sasaki and colleagues Jin'ichi of Hirosaki University in northern Japan, squid ink can activates white blood cells to fight cancer. The compound is thought to have anti-cancer activity should be tested first in animals before being developed further. The purpose of this study are determining and comparing the toxicity of the components of biological akti...

  11. CONSUMPTIONS RATES OF SUMMER FLOUNDER LARVAE ON ROTIFER AND BRINE SHRIMP PREY DURING LARVAL REARING

    Science.gov (United States)

    Larval summer flounder Paralichthys dentatus were hatched and reared through metamorphosis in the laboratory. At several points in the rearing cycle, larvae were removed from their rearing chambers and placed in small bowls, where they were fed known quantities of the rotifer Bra...

  12. Title: Antimicrobial activity and brine shrimp toxicity of extracts of a ...

    African Journals Online (AJOL)

    Owner

    microbes Bacillus subtilis and Candida albicans were used to test, the ... The phenolic compounds were recorded high up to 187.45 GAE mg/g, Я-carotene ... lignocellulose degradation in nature. In .... paper and the filtrate dried at 50 ºC for 72.

  13. Utilization of Black Tiger Shrimp Flesh Waste for Pop Shrimp Processing

    Directory of Open Access Journals (Sweden)

    Hari Eko Irianto

    2017-05-01

    Full Text Available A study on the utilization of black  tiger shrimp (Penaeus monodon flesh waste in the processing of pop shrimp has been carried out.  So far, shrimp flesh waste is used for the production of shrimp paste, and shrimp cracker.   The objective of this study was to develop a fomula for pop shrimp production using shrimp flesh waste. Experimental design applied in this study was three-variables mixture design, in which variables observed were shrimp flesh waste, surimi and tapioca flour. Pop shrimp obtained was evaluated for sensory properties. The best product processed using a selected formula was analysed chemically and microbiologically, particularly for proxymate composition and total plate count respectively. Selected formula of pop shrimp consisted of 50.91% shrimp flesh waste, 18.18% surimi, 3.64% tapioca flour, 10.91% onion, 2.18% garlic, 0.73% pepper powder, 1.45% sugar, 0.36% monosodium glutamate, 0.73% ginger, 1.45% salt, 4.44% butter mix, 1.38% corn flour (maizena and 3.64% bread crumb. Proxymate composition of the best pop shrimp was 70.52% moisture, 0.73% ash, 0.39% fat, and 7.44% protein, while microbiological load in terms of total plate count was 3.3x103 colonies/g.

  14. Brine Sampling and Evaluation Program: Phase 1 report

    International Nuclear Information System (INIS)

    Deal, D.E.; Case, J.B.

    1987-01-01

    This interim report presents preliminary data obtained in the course of the WIPP Brine Sampling and Evaluation Program. The investigations focus on the brine present in the near-field environment around the WIPP underground workings. Although the WIPP underground workings are considered dry, small amounts of brine are present. This amount of brine is not unexpected in rocks of marine sedimentary origin. Part of that brine can and does migrate into the repository in response to pressure gradients, at essentially isothermal conditions. These small volumes of brine have little effect on the day-to-day operations, but are pervasive throughout the repository and may contribute enough moisture over a period of years to affect resaturation and repressurization after sealing and closure. Gas bubbles are observed in many of the brine occurrences. Gas is also known to exsolve from solution as the brine is poured from container to container. 68 refs., 9 figs., 2 tabs

  15. Portable brine evaporator unit, process, and system

    Science.gov (United States)

    Hart, Paul John; Miller, Bruce G.; Wincek, Ronald T.; Decker, Glenn E.; Johnson, David K.

    2009-04-07

    The present invention discloses a comprehensive, efficient, and cost effective portable evaporator unit, method, and system for the treatment of brine. The evaporator unit, method, and system require a pretreatment process that removes heavy metals, crude oil, and other contaminates in preparation for the evaporator unit. The pretreatment and the evaporator unit, method, and system process metals and brine at the site where they are generated (the well site). Thus, saving significant money to producers who can avoid present and future increases in transportation costs.

  16. Microbial Diseases in Shrimp Aquaculture

    Digital Repository Service at National Institute of Oceanography (India)

    Karunasagar, Iddya; Karunasagar, Indrani; Umesha, R.K.

    for detection of rhabdovirus of penaeid shrimp in the tissue of infected animals (Nadala et al. 1992). Management of viral diseases Since there is no known treatment for viral diseases, the major strategy for disease management is avoidance. However, in practice... suspected that natural food and artificial feed quality, husbandry practices, water quality and presence of toxic substances from chemical prophylactics are responsible for SHG, but no specific cause has been perpointed so far (Lavilla-Pitogo et al., 2002...

  17. Biomass production of Artemia in air-water-lift raceway system

    Digital Repository Service at National Institute of Oceanography (India)

    Royan, J.P.; Vijayaraghavan, S.; Krishnakumari, L

    - from Venkataraman, 1983). Crude Protein (N x6.25) Lipid Carbohydrate Crude Fiber Ash Moisture 55-65 2-6 10-15 1-4 6-15 5-10 o· ClS'- •• •• lM6M StrGft •~S,I LUlu STrOI,. • tOO A eo 10 ~ • i:f e & .... 0. eo E I w ,. .. en c eo en ~ c • z " 2 a 1M 0 U ji...!:Ta:Vijayaraghavan, Wafar andRoyail~l~n~n.Good growthrates wereobserved in Penaeui indicus, P. monoceros andM. monodon when fed with live adult Artemia (Royan, Sumitra-Vijayaraghavanand Krishna Kumari, 1987). Thus adult Artemia biomass can be used as an ingredient...

  18. Slumping of brine mounds : bounds on behaviour

    NARCIS (Netherlands)

    Philips, J.R.; Duijn, van C.J.

    1996-01-01

    Two modifications of the approximate analysis of interface motion during two-fluid density-driven flows of De Josselin de Jong (Proc. Euromech., 143: 75–82, 1981) are applied to the slumping of finite two-dimensional and axisymmetric brine mounds. Both lead to simple similarity solutions. One

  19. Evaluación de dietas artificiales con artemia adulta en la maduración y reproducción de penaeus vannamei

    OpenAIRE

    Zambrano Carranza, Byron R.

    1999-01-01

    Evaluación de dietas artificiales con Artemia adulta en la maduración y reproducción de Penaeus vannamei Un experimento de maduración y reproducción de P. vannamei fue llevado a cabo en tanques de 18 toneladas para (a) comprobar los efectos de la Artemia adulta como ingrediente principal en dietas artificiales, (b) investigar si existen diferencias entre la implementación de liofilizado de Artemia adulta y Artemia adulta congelada, y finalmente (c), comparar estos efectos y resultados con ...

  20. Toxicities of diuron and irgarol on the hatchability and early stage development of Artemia salina

    OpenAIRE

    ALYÜRÜK, Hakan; ÇAVAŞ, Levent

    2013-01-01

    Booster biocides are widely used in antifouling paints as bioactive agents against fouling organisms. In previously published reports, acute toxicity tests on Artemia salina (Linnaeus, 1758) were only focused on a part of the life cycle of the organism. The aim of this study was to investigate the toxicities of diuron and irgarol on the hatching stage of A. salina. According to the results, diuron significantly decreased the hatching percentage of A. salina cysts and prevented the hatching of...

  1. The study of fkbp and ubiquitin reveals interesting aspects of Artemia stress history.

    Science.gov (United States)

    Maniatsi, Stefania; Farmaki, Theodora; Abatzopoulos, Theodore J

    2015-08-01

    Research on stress responses in animals has increased greatly during the last decades. Though most studies focus on the cellular and molecular bases of the stress response mechanisms, the ecological and evolutionary aspects of stress responses gain more and more interest. Here, we use species and parthenogenetic strains of the genus Artemia, an extremophile model organism, to study, for the first time, a protein well known for its chaperone activity and its involvement in stress responses. More specifically, transcription and protein accumulation of an FK506-Binding Protein (FKBP) homologue were investigated under heat and salt stresses. Additionally, the mRNA levels of ubiquitin, a heat-inducible protein related to the proteasomal pathway, were quantitated under these conditions. Biochemical and phylogenetic analyses showed that the studied FKBP orthologue is a typical representative of the family that clusters with other crustacean sequences. The expression was increased in both fkbp and ubiquitin genes after salt and heat stresses. However, our results in combination with the fact that Artemia species and parthenogenetic strains, selected for this study, exhibit different heat or salt tolerance provide useful hints about the evolutionary significance of FKBP and ubiquitin. Regarding FKBP, mRNA expression and protein accumulation seem to depend on the environmental conditions and the evolutionary history of each Artemia population while ubiquitin has a clear and more conserved role under heat shock. Copyright © 2015 Elsevier Inc. All rights reserved.

  2. Transfer and accumulation of 106Ru from phytoplankton (Dunaliella bioculata) to zooplankton (Artemia salina)

    International Nuclear Information System (INIS)

    Izumo, Yoshiro; Takase, Akira

    1982-01-01

    106 Ru is one of the most abundant radionuclides to be released from the unclear-spent fuel reprocessing plant to oceans. This paper deals with transfer and accumulation of the radionuclide from phytoplankton (Dunaliella) to zooplankton (Artemia) in the graizing food chain of marine organisms. Each plankton was reared in the radioactive sea water A (filtrated and circulated through sandy mud, and the radioactive concentration decreased and atteined to constant after addition of 106 Ru), B (not filtrated and circulated, and the radioactive concentration decreased and atteined to constant) and C (not filtrated and circulated, the radioactive concentration decreased, but did not attein to constant), respectively, and investigated accumulations of 106 Ru. Then, accumulations of 106 Ru in Artemia by the ingestion of each diet (Dunaliella) contaminated with 106 Ru in each water, were investigated and compared with those from each water. Concentration factors of 106 Ru for the planktons reared in the radioactive sea waters were different among these waters. The difference can be attributable to that of physicochemical states of 106 Ru in the waters. It was found that 106 Ru would be accumulated by Artemia from the water A about 10 times higher than from the contaminated diet (Dunaliella) in this water, however from the contaminated diet (Dunaliella) in the water B about 2.2 times higher than from the last water. (author)

  3. TENDENCIES AND PECULIARITIES OF SHRIMP FEED PRODUCTION

    Directory of Open Access Journals (Sweden)

    LIUDMYLA V. FIHURSKA

    2018-04-01

    Full Text Available Aquaculture is food sector, which is growing rapidly in the last 25 years with annual growth rate 8,2 . One of the mostperspective branches of aquaculture is shrimp farming. The cost of feeds is up to 80% of the cost of shrimp breeding, so providingthe industry with high-quality feeds is the important goal of the feed industry in all over the world. The theoretical research was devotedto the task of compound shrimp feed production. In order to satisfy shrimp requirements, shrimp feeding systems were shown.Existing shrimp breeding systems are shown as different from the type of reservoirs (static / running water, indoor or outdoor, feedingmethods, and the species of grown shrimp. Features of the nutritional standards for freshwater and saltwater shrimps were analyzed.Nutrient requirements of shrimp have been changed through shrimp life-cycle. The shrimp life-cycle was shown.World leaders-producers of compound mixed feeds for shrimps were shown. The analysis of pellet size and nutritional valueof compound mixed feeds of crude protein content in prestart, starter, grower and finish periods of cultivation and in accordancewith the system of cultivation and feeding shrimp (intensive, extensive, semi-intensive is carried out. The requirements for the contentof main minerals, vitamins and restrictions to the content of crude fiber are given. Traditional ingredients are described. Bindersand preservatives, which are used for shrimp feeds, are shown and subscribed. In raw material the main problem is the need to ensurehigh protein content in the shrimp feed recipes. Because of many factors, fish meal quantity should be reduced in recipes. Becauseof its attractive amino acid content, availability and relatively affordable price, soybean meal and soy concentrates have receivedincreasing attention as substitutes for marine animal meals.The features of technological lines and processes of production of mixed feeds for shrimp are shown.Еhe advantages and

  4. Expected brine movement at potential nuclear waste repository salt sites

    International Nuclear Information System (INIS)

    McCauley, V.S.; Raines, G.E.

    1987-08-01

    The BRINEMIG brine migration code predicts rates and quantities of brine migration to a waste package emplaced in a high-level nuclear waste repository in salt. The BRINEMIG code is an explicit time-marching finite-difference code that solves a mass balance equation and uses the Jenks equation to predict velocities of brine migration. Predictions were made for the seven potentially acceptable salt sites under consideration as locations for the first US high-level nuclear waste repository. Predicted total quantities of accumulated brine were on the order of 1 m 3 brine per waste package or less. Less brine accumulation is expected at domal salt sites because of the lower initial moisture contents relative to bedded salt sites. Less total accumulation of brine is predicted for spent fuel than for commercial high-level waste because of the lower temperatures generated by spent fuel. 11 refs., 36 figs., 29 tabs

  5. Comparison of bioassays using the anostracan crustaceans Artemia salina and Thamnocephalus platyurus for plant extract toxicity screening

    Directory of Open Access Journals (Sweden)

    Pablo Mayorga

    2010-10-01

    Full Text Available Three lethality bioassays, using the salt-water crustacean Artemia salina Leach, Artemiidae, (conventional 96 microwell plate test and the Artoxkit M microbiotest and the freshwater crustacean Thamnocephalus platyurus Packard, Thamnocephalidae, (Thamnotoxkit F microbiotest, were compared using extracts of ten Guatemalan plant species. It was previously observed that five of them have anti-Artemia activity. These were: Solanum americanum Mill., Solanaceae, Gliricidia sepium (Jacq. Kunth ex Walp., Fabaceae, Neurolaena lobata (L. Cass., Asteraceae, Petiveria alliacea L., Phytolaccaceae, and Ocimum campechianum Mill., Lamiaceae. The five others: Curatella americana L., Dilleniaceae, Prunus barbata Koehne, Rosaceae, Quercus crispifolia Trel., Fagaceae, Rhizophora mangle L., Rhizophoraceae, and Smilax domingensis Willd., Smilacaceae, do not. All plants without anti-Artemia activity had no lethal effects in both assays with A. salina. For the plants with anti-Artemia activity the Artoxkit M was not sensitive to G. sepium and the conventional Artemia test was not sensitive to S. americanum, G. sepium and N. lobata. All the plant extracts, except for that of C. americana, had lethal effects on T. platyurus and the lethal median concentration (LC50 levels for this organism were in all cases substantially lower than those of the salt-water test species. This study revealed that T. platyurus is a promising test species worth further in depth investigation for toxicity screening of plant extracts with potential medicinal properties.

  6. MBV infection in various stages of black tiger shrimp (Penaeus monodon

    Directory of Open Access Journals (Sweden)

    Supamattaya, K.

    2005-02-01

    Full Text Available MBV infection in various stages of Penaeus monodon were studied. In hatcheries, MBV infection was detected early in nauplii stage using PCR technique, whereas rates of infection of 11.15-49.50% were observed in PL1 using histological technique, rising up to 15.26-100% in PL 10. In earthen ponds, the infection in PL15 was initially in the range of 68.68-96.00%. The infection was decreased toward the end of the first, second and third month of rearing period ranging between 13.63-54.83%. The laboratory trial showed that types of feed might affect the rate of MBV infection of larvae. Postlarva fed with artemia showed lowest infection rate at 29.41±7.98%, whereas the infection rates of shrimp fed with minced cockle flesh and commercial feed were 39.09±12.08% and 52.81±11.91, respectively. In stress test trial, a significant MBV infection was detected in the group of larvae that were raised with 25ºC and 34ºC and the salinity at 6 ppt and 18 ppt for 12 hours then rearing in normal condition for 3 days. In the 24 hour-stress trial, and transferred to normal condition for 7 day, the groups that were exposed to stress conditions had significantly higher rates of infection than the control group (p<0.05. The 24 hour - transportationcondition resulted in highest MBV infection rate (73.61±1.25%. From the present study, it was concluded that MBV infection in larvae from hatcheries increases with period of rearing and stress exposure, but the infection tended to decreased with rearing period in earthen pond condition. Proper feeding management and prevention of stress conditions could reduce of MBV infection in black tiger shrimp.

  7. Approach to recover strategic metals from brines

    Energy Technology Data Exchange (ETDEWEB)

    Raber, E.; Harrar, J.; Gregg, D.

    1981-09-16

    The objective of the proposed research is to evaluate hypersaline brines from geothermal sources and salt domes as possible sources for some strategic metals. This research is suggested because several previous analyses of brine from geothermal wells in the Imperial Valley, California, and from Gulf Coast salt domes, indicate near commercial values for platinum as well as other metals (i.e., gold, silver). Extraction of the platinum should be technically feasible. A research program should include more complete systematic sampling and analysis for resource delineation, followed by bench-scale investigation of several potential extraction processes. This could be followed by engineering feasibility and design studies, for extraction of the metals either as a by-product of other operations or in a stand-alone process.

  8. Formation of brine channels in sea ice.

    Science.gov (United States)

    Morawetz, Klaus; Thoms, Silke; Kutschan, Bernd

    2017-03-01

    Liquid salty micro-channels (brine) between growing ice platelets in sea ice are an important habitat for CO 2 -binding microalgaea with great impact on polar ecosystems. The structure formation of ice platelets is microscopically described and a phase field model is developed. The pattern formation during solidification of the two-dimensional interstitial liquid is considered by two coupled order parameters, the tetrahedricity as structure of ice and the salinity. The coupling and time evolution of these order parameters are described by a consistent set of three model parameters. They determine the velocity of the freezing process and the structure formation, the phase diagram, the super-cooling and super-heating region, and the specific heat. The model is used to calculate the short-time frozen micro-structures. The obtained morphological structure is compared with the vertical brine pore space obtained from X-ray computed tomography.

  9. Modeling of brine migration in halite

    International Nuclear Information System (INIS)

    Cheung, H.; Fuller, M.E.; Gaffney, E.S.

    1979-01-01

    When canisters containing radwastes are emplaced in a repository the heat produced by the decaying radwaste will cause moderate thermal gradients to develop which will cause the brine present in a halite medium (salt deposits) to accumulate around the canister. Four different models of the migration process have been reviewed to determine their suitability as a working model. One model predicts that inclusions smaller than 0.1 mm dimension probably will not migrate. The other models do not consider size as a factor. Thermal diffusion (Soret effect) is considered insignificant in three models, while in the fourth model it is added to the concentration diffusion term. The following conclusions can be made: Temperature is the most significant parameter in all models and must be known as a function of time, and distance from the canister. All four models predict about the same migration velocity for it is a given set of conditions; for 100 0 C and 1 0 C/cm thermal gradient, it is 3.0, 4.8, 5.6 and 6.4 mm/y. Diffusion of ions through the brine inclusions is the rate controlling mechanism. The difference between the thermal gradients in the liquid and in the solid should always be considered and is a function of droplet shape. The model based upon work by Nernst is easiest to use, but it predicts the lowest migration rate. The maximum volume of pure brine accumulated at the canister surface would be less than 20-40 liters in 50 years, for a canister initial thermal power of 3.5 kW.Bitterns would migrate proportionately less volume. A computer code, BRINE, was developed to make these calculations by means of any of the four models

  10. Probiotics as Antiviral Agents in Shrimp Aquaculture

    Directory of Open Access Journals (Sweden)

    Bestha Lakshmi

    2013-01-01

    Full Text Available Shrimp farming is an aquaculture business for the cultivation of marine shrimps or prawns for human consumption and is now considered as a major economic and food production sector as it is an increasingly important source of protein available for human consumption. Intensification of shrimp farming had led to the development of a number of diseases, which resulted in the excessive use of antimicrobial agents, which is finally responsible for many adverse effects. Currently, probiotics are chosen as the best alternatives to these antimicrobial agents and they act as natural immune enhancers, which provoke the disease resistance in shrimp farm. Viral diseases stand as the major constraint causing an enormous loss in the production in shrimp farms. Probiotics besides being beneficial bacteria also possess antiviral activity. Exploitation of these probiotics in treatment and prevention of viral diseases in shrimp aquaculture is a novel and efficient method. This review discusses the benefits of probiotics and their criteria for selection in shrimp aquaculture and their role in immune power enhancement towards viral diseases.

  11. Cationic antimicrobial peptides in penaeid shrimp.

    Science.gov (United States)

    Tassanakajon, Anchalee; Amparyup, Piti; Somboonwiwat, Kunlaya; Supungul, Premruethai

    2011-08-01

    Penaeid shrimp aquaculture has been consistently affected worldwide by devastating diseases that cause a severe loss in production. To fight a variety of harmful microbes in the surrounding environment, particularly at high densities (of which intensive farming represents an extreme example), shrimps have evolved and use a diverse array of antimicrobial peptides (AMPs) as part of an important first-line response of the host defense system. Cationic AMPs in penaeid shrimps composed of penaeidins, crustins, and anti-lipopolysaccharide factors are comprised of multiple classes or isoforms and possess antibacterial and antifungal activities against different strains of bacteria and fungi. Shrimp AMPs are primarily expressed in circulating hemocytes, which is the main site of the immune response, and hemocytes expressing AMPs probably migrate to infection sites to fight against pathogen invasion. Indeed, most AMPs are produced as early as the nauplii developmental stage to protect shrimp larvae from infections. In this review, we discuss the sequence diversity, expression, gene structure, and antimicrobial activities of cationic AMPs in penaeid shrimps. The information available on antimicrobial activities indicates that these shrimp AMPs have potential therapeutic applications in the control of disease problems in aquaculture.

  12. Evolution of hydrologic systems and brine geochemistry in a deforming salt medium: Data from WIPP brine seeps

    International Nuclear Information System (INIS)

    Deal, D.E.; Roggenthen, W.M.

    1991-01-01

    The Brine Sampling and Evaluation Program (BSEP) is a formalized continuation of studies that began in 1982 as part of the Site Validation Program. The program was established in 1985. The mission was to document and investigate the origins, hydraulic characteristics, extent, and composition of brine occurrences in the Permian Salado Formation and the seepage of that brine into the WIPP excavations. This document focuses on the cumulative data obtained from the BSEP. The overall activities of the BSEP described and quantified the brine. It includes documentation and study of brine inflow into boreholes in the facility. The BSEP investigated the occurrence and development of brine weeps, crusts, and brine geochemistry. The presence of salt-tolerant bacteria in the workings was recorded and their possible interactions with experiments and operations, was assessed. The formation properties associated with the occurrence of brine was characterized. The determination of formation properties included the water content of various geologic units, direct examination of these units in boreholes using a video camera system, and measurement of electrical properties relatable to the brine contents. Modeling examined the interaction of salt deformation near the workings and the flow of brine through the deforming rocks. 34 refs

  13. The Brine Sampling and Evaluation Program (PSEP) at WIPP

    International Nuclear Information System (INIS)

    Deal, D.E.; Roggenthen, W.M.

    1989-01-01

    The Permian salt beds of the WIPP facility are virtually dry. The amount of water present in the rocks exposed in the excavations that is free to migrate under pressure gradients was estimated by heating salt samples to 95 degrees C and measuring weight loss. Clear balite contains about 0.22 weight percent water and the more argillaceous units average about 0.75 percent. Measurements made since 1984 as part of the Brine Sampling and Evaluation Program (BSEP) indicate that small amounts of this brine can migrate into the excavations and does accumulate in the underground environment. Brine seepage into drillholes monitored since thy were drilled show that brine seepage decreases with time and that many have dried up entirely. Weeping of brine from the walls of the repository excavations also decreases after two or more years. Chemical analyses of brines shows that they are sodium-chloride saturated and magnesium-rich

  14. Evolutionary origin and phylogeography of the diploid obligate parthenogen Artemia parthenogenetica (Branchiopoda: Anostraca.

    Directory of Open Access Journals (Sweden)

    Joaquín Muñoz

    2010-08-01

    Full Text Available Understanding the evolutionary origin and the phylogeographic patterns of asexual taxa can shed light on the origin and maintenance of sexual reproduction. We assessed the geographic origin, genetic diversity, and phylogeographic history of obligate parthenogen diploid Artemia parthenogenetica populations, a widespread halophilic crustacean.We analysed a partial sequence of the Cytochrome c Oxidase Subunit I mitochondrial gene from an extensive set of localities (including Eurasia, Africa, and Australia, and examined their phylogeographic patterns and the phylogenetic relationships of diploid A. parthenogenetica and its closest sexual relatives. Populations displayed an extremely low level of mitochondrial genetic diversity, with one widespread haplotype shared by over 79% of individuals analysed. Phylogenetic and phylogeographic analyses indicated a multiple and recent evolutionary origin of diploid A. parthenogenetica, and strongly suggested that the geographic origin of parthenogenesis in Artemia was in Central Asia. Our results indicate that the maternal sexual ancestors of diploid A. parthenogenetica were an undescribed species from Kazakhstan and A. urmiana.We found evidence for multiple origin of parthenogenesis in Central Asia. Our results indicated that, shortly after its origin, diploid A. parthenogenetica populations underwent a rapid range expansion from Central Asia towards the Mediterranean region, and probably to the rest of its current geographic distribution. This contrasts with the restricted geographic distribution, strong genetic structure, and regional endemism of sexual Artemia lineages and other passively dispersed sexual continental aquatic invertebrates. We hypothesize that diploid parthenogens might have reached their current distribution in historical times, with a range expansion possibly facilitated by an increased availability of suitable habitat provided by anthropogenic activities, such as the spread of solar

  15. Evaluation of Alpha and Gamma Aluminum Oxide Nanoparticle Accumulation, Toxicity and Depuration in Artemia Salina Larvae

    Science.gov (United States)

    Ates, Mehmet; Demir, Veysel; Arslan, Zikri; Daniels, James; Farah, Ibrahim O.; Bogatu, Corneliu

    2014-01-01

    In this study, Artemia salina (crustacean filter feeders) larvae were used as a test model to investigate the toxicity of aluminum oxide nanoparticles (Al2O3 NPs) on marine microorganisms. The uptake, toxicity and elimination of α-Al2O3 (50 nm and 3.5 μm) and γ-Al2O3 (5 nm and 0.4 μm) NPs were studied. Twenty-four and ninety-six hour exposures of different concentrations of Al2O3 NPs to Artemia larvae were conducted in a seawater medium. When suspended in water, Al2O3 NPs aggregated substantially with the sizes ranging from 6.3 nm to > 0.3 μm for spherical NPs, and from 250 to 756 nm for rod-shaped NPs. The phase contrast microscope images revealed that NPs deposited inside the guts as aggregates. ICP-MS analysis showed that large particles (3.5 μm α-Al2O3) were not taken up by Artemia, while fine NPs (0.4 μm γ-Al2O3) and ultra-fine NPs (5 nm γ-Al2O3 and 50 nm α-Al2O3) accumulated substantially. Differences in toxicity were detected as changing with NP size and morphology. The malondialdehyde (MDA) levels indicated that smaller γ-Al2O3 (5 nm) NPs were more toxic than larger γ-Al2O3 (0.4 μm) particulates in 96 h. The highest mortality was measured as 34% in 96 h for γ-Al2O3 NPs (5 nm) at 100 mg/L (LC50 > 100 mg/L). γ-Al2O3 NPs were more toxic than α-Al2O3 NPs at in all conditions. PMID:24753078

  16. OUT Success Stories: Chemical Treatments for Geothermal Brines

    International Nuclear Information System (INIS)

    Burr, R.

    2000-01-01

    DOE research helped develop the large, untapped geothermal resource beneath the Salton Sea in California's Imperial Valley. The very hot brines under high pressure make them excellent for electric power production. The brines are very corrosive and contain high concentrations of dissolved silica. DOE worked with San Diego Gas and Electric Company to find a solution to the silica-scaling problem. This innovative brine treatment eliminated scaling and made possible the development of the Salton Sea geothermal resource

  17. Phaeobacter inhibens as Probiotic Bacteria in Non-Axenic Artemia and Algae Cultures

    DEFF Research Database (Denmark)

    Grotkjær, Torben; D'Alvise, Paul; Bentzon-Tilia, Mikkel

    2015-01-01

    Bacterial diseases are a major constraint in aquaculture, especially in larviculture. Antibiotics that can control pathogens should be avoided due to risk of antibiotic resistance. We have shown in axenic systems of live larval feed that marine Roseobacter clade bacteria can antagonize fish patho...... demonstrates that probiotic bacteria can be introduced at the stage of live feed and have a pathogen reducing effect in both an Artemia and a D. tertiolecta challenge setup. This can potentially limit the subsequent use of antibiotics for control of pathogenic bacteria....

  18. Evaluation of Brine Processing Technologies for Spacecraft Wastewater

    Science.gov (United States)

    Shaw, Hali L.; Flynn, Michael; Wisniewski, Richard; Lee, Jeffery; Jones, Harry; Delzeit, Lance; Shull, Sarah; Sargusingh, Miriam; Beeler, David; Howard, Jeanie; hide

    2015-01-01

    Brine drying systems may be used in spaceflight. There are several advantages to using brine processing technologies for long-duration human missions including a reduction in resupply requirements and achieving high water recovery ratios. The objective of this project was to evaluate four technologies for the drying of spacecraft water recycling system brine byproducts. The technologies tested were NASA's Forward Osmosis Brine Drying (FOBD), Paragon's Ionomer Water Processor (IWP), NASA's Brine Evaporation Bag (BEB) System, and UMPQUA's Ultrasonic Brine Dewatering System (UBDS). The purpose of this work was to evaluate the hardware using feed streams composed of brines similar to those generated on board the International Space Station (ISS) and future exploration missions. The brine formulations used for testing were the ISS Alternate Pretreatment and Solution 2 (Alt Pretreat). The brines were generated using the Wiped-film Rotating-disk (WFRD) evaporator, which is a vapor compression distillation system that is used to simulate the function of the ISS Urine Processor Assembly (UPA). Each system was evaluated based on the results from testing and Equivalent System Mass (ESM) calculations. A Quality Function Deployment (QFD) matrix was also developed as a method to compare the different technologies based on customer and engineering requirements.

  19. Extreme morphologies of mantis shrimp larvae

    DEFF Research Database (Denmark)

    Haug, Carolin; Ahyong, Shane T.; Wiethase, Joris H.

    2016-01-01

    Larvae of stomatopods (mantis shrimps) are generally categorized into four larval types: antizoea, pseudozoea (both representing early larval stages), alima and erichthus (the latt er two representing later larval stages). These categories, however, do not refl ect the existing morphological...

  20. Gulf of Mexico Shrimp Permit Gear Survey

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Data set contains annual vessel gear characterization of permit holders shrimp vessel. Data includes net type, TED type, BRD type, etc.

  1. Selective Recovery of Metals from Geothermal Brines

    Energy Technology Data Exchange (ETDEWEB)

    Ventura, Susanna [SRI International, Menlo Park, CA (United States); Bhamidi, Srinivas [SRI International, Menlo Park, CA (United States); Hornbostel, Marc [SRI International, Menlo Park, CA (United States); Nagar, Anoop [SRI International, Menlo Park, CA (United States); Perea, Elisabeth [SRI International, Menlo Park, CA (United States)

    2016-12-16

    The objective of this project was to determine the feasibility of developing a new generation of highly selective low-cost ion-exchange resins based on metal-ion imprinted polymers for the separation of metals from geothermal fluids. Expansion of geothermal energy production over the entire U.S. will involve exploitation of low-to-medium temperature thermal waters. Creating value streams from the recovery of critical and near-critical metals from these thermal waters will encourage geothermal expansion. Selective extraction of metals from geothermal fluids is needed to design a cost-effective process for the recovery of lithium and manganese-two near-critical metals with well-known application in the growing lithium battery industry. We have prepared new lithium- and manganese-imprinted polymers in the form of beads by crosslinking polymerization of a metal polymerizable chelate, where the metal acts as a template. Upon leaching out the metal template, the crosslinked polymer is expected to leave cavities defined by the ligand functional group with enhanced selectivity for binding the template metal. We have demonstrated that lithium- and manganese-imprinted polymer beads can be used as selective solid sorbents for the extraction of lithium and manganese from brines. The polymers were tested both in batch extractions and packed bed lab-scale columns at temperatures of 45-100°C. Lithium-imprinted polymers were found to have Li+ adsorption capacity as high as 2.8 mg Li+/g polymer at 45°C. Manganese-imprinted polymers were found to have a Mn2+ adsorption capacity of more than 23 mg Mn2+/g polymer at 75°C. The Li+ extraction efficiency of the Li-imprinted polymer was found to be more that 95% when a brine containing 390 ppm Li+, 410 ppm Na+, and 390 ppm K+ was passed through a packed bed of the polymer in a lab-scale column at 45°C. In brines containing 360 ppm Li

  2. Consolidation and permeability of salt in brine

    International Nuclear Information System (INIS)

    Shor, A.J.; Baes, C.F. Jr.; Canonico, C.M.

    1981-07-01

    The consolidation and loss of permeability of salt crystal aggregates, important in assessing the effects of water in salt repositories, has been studied as a function of several variables. The kinetic behavior was similar to that often observed in sintering and suggested the following expression for the time dependence of the void fraction: phi(t) = phi(0) - (A/B)ln(1 + Bt/z(0) 3 ), where A and B are rate constants and z(0) is initial average particle size. With brine present, A and phi(0) varied linearly with stress. The initial void fraction was also dependent to some extent on the particle size distribution. The rate of consolidation was most rapid in brine and least rapid in the presence of only air as the fluid. A brine containing 5 m MgCl 2 showed an intermediate rate, presumably because of the greatly reduced solubility of NaCl. A substantial wall effect was indicated by an observed increase in the void fraction of consolidated columns with distance from the top where the stress was applied and by a dependence of consolidation rate on the column height and radius. The distance through which the stress fell by a factor of phi was estimated to change inversely as the fourth power of the column diameter. With increasing temperature (to 85 0 C), consolidation proceeded somewhat more rapidly and the wall effect was reduced. The permeability of the columns dropped rapidly with consolidation, decreasing with about the sixth power of the void fraction. In general, extrapolation of the results to repository conditions confirms the self-sealing properties of bedded salt as a storage medium for radioactive waste

  3. Brine Transport Experiments in Granular Salt

    Energy Technology Data Exchange (ETDEWEB)

    Jordan, Amy B. [Los Alamos National Lab. (LANL), Los Alamos, NM (United States); Boukhalfa, Hakim [Los Alamos National Lab. (LANL), Los Alamos, NM (United States); Caporuscio, Florie Andre [Los Alamos National Lab. (LANL), Los Alamos, NM (United States); Stauffer, Philip H. [Los Alamos National Lab. (LANL), Los Alamos, NM (United States)

    2016-06-06

    To gain confidence in the predictive capability of numerical models, experimental validation must be performed to ensure that parameters and processes are correctly simulated. The laboratory investigations presented herein aim to address knowledge gaps for heat-generating nuclear waste (HGNW) disposal in bedded salt that remain after examination of prior field and laboratory test data. Primarily, we are interested in better constraining the thermal, hydrological, and physicochemical behavior of brine, water vapor, and salt when moist salt is heated. The target of this work is to use run-of-mine (RoM) salt; however during FY2015 progress was made using high-purity, granular sodium chloride.

  4. Pressure-driven brine migration in a salt repository

    International Nuclear Information System (INIS)

    Hwang, Y.; Chambre, P.L.; Pigford, T.H.; Lee, W.W.L.

    1989-01-01

    The traditional view is that salt is the ideal rock for isolation of nuclear waste because it is ''dry'' and probably ''impermeable.'' The existence of salt through geologic time is prima facie evidence of such properties. Experiments and experience at potential salt sites for geologic repositories have indicated that while porosity and permeability of salt are low, the salt may be saturated with brine. If this hypothesis is correct, then it is possible to have brine flow due to pressure differences within the salt. If there is pressure-driven brine migration in salt repositories then it is paramount to know the magnitude of such flow because inward brine flow would affect the corrosion rate of nuclear waste containers and outward brine flow might affect radionuclide transport rates. Brine exists in natural salt as inclusions in salt crystals and in grain boundaries. Brine inclusions in crystals move to nearby grain boundaries when subjected to a temperature gradient, because of temperature-dependent solubility of salt. Brine in grain boundaries moves under the influence of a pressure gradient. When salt is mined to create a waste repository, brine from grain boundaries will migrate into the rooms, tunnels and boreholes because these cavities are at atmospheric pressure. After a heat-emitting waste package is emplaced and backfilled, the heat will impose a temperature gradient in the surrounding salt that will cause inclusions in the nearby salt to migrate to grain boundaries within a few years, adding to the brine that was already present in the grain boundaries. The formulation of brine movement with salt as a thermoelastic porous medium, in the context of the continuum theory of mixtures, has been described. In this report we show the mathematical details and discuss the results predicted by this analysis

  5. Evaluation of the toxicity of the methanolic extract of Caesalpinia Pyramidalis, subjected to gamma radiation, compared to saline artemias; Avaliacao da toxicidade do extrato metanolico de Caesalpinia Pyramidalis, submetido a radiacao gama, frente a artemias salinas

    Energy Technology Data Exchange (ETDEWEB)

    Pessoa, Juanna G.; Santos, Mariana L.O.; Costa, Michelle C.A.; Siqueira, Williams N.; Silva, Luanna R.S.; Cabral, Daniela L.V.; Amancio, Francisco F.; Melo, Ana M.M.A., E-mail: marianasantos_ufpe@hotmail.com [Universidade Federal de Pernambuco (UFPE), Recife, PE (Brazil)

    2013-07-01

    The present study aimed to investigate the possible effect of irradiation of 60 Co in methanol extract of Caesalpinia pyramidalis (catingueira) with function of reducing the toxicity of the extract at concentrations of 1000, 500, 250 and 125 ppm irradiating them with doses of 5 kGy 7 kGy and 10 kGy, using lethality tests with saline Artemias.

  6. Competitiveness of Indonesian Shrimp Compare with Thailand Shrimp in Export Market

    Directory of Open Access Journals (Sweden)

    Lina Asmara Wati

    2013-09-01

    Full Text Available With the water area accounts for 81 percent of the total area, fishery is a major business in Indonesia. As one of the world's largest shrimp exporting countries, Indonesia also relies on the revenues of shrimp exports in the international markets. This research compared the competitiveness of shrimp export from Indonesia and Thailand to Japan and US. The indicator adopted in this research was the RCA index. In this research collected secondary data from 1989 to 2010 for analysis. Results showed RCA values of Indonesia were higher than 1, which indicates advantaged competitiveness of Indonesia shrimp exports in the liberalized markets. Nevertheless, compared with Thailand, Indonesia remained relatively disadvantaged. To reinforce the Indonesian shrimp export, necessary infrastructure, such as networks shipment or delivery, packaging and transportation, must be improved. Further researches to determine the factors affecting competitiveness of export shrimp in liberalized market, make and analyze for the policy strategy formulation of Indonesia shrimp export in liberalized market are also advised.Keywords: Competitiveness; Indonesian Shrimp Export; RCA.

  7. Assessment of endemic microalgae as potential food for Artemia franciscana culture

    Directory of Open Access Journals (Sweden)

    Juan M Pacheco-Vega

    2015-03-01

    Full Text Available In this study, five microalgal strains were isolated from Bahía de La Paz, Baja California Sur, Mexico and identified as Grammatophora sp., Navícula sp., Rhabdonema sp., Schizochytrium sp., and Nitzschia sp., and their evaluation as potential food for Artemia franciscana. The isolated strains were cultured outdoors and harvested after four days. Chaetoceros muelleri was cultured under laboratory conditions and used as control. The protein, lipid, and carbohydrate composition and the fatty acid profiles of the strains were determined by gas chromatography. To assess the effect of microalgal strains on A. franciscana, decapsulated cysts were cultured at outdoor conditions in 15 L containers. The experiment was conducted for twelve days. Samples from the five different feeding treatments were taken at the beginning and end of the experiment to assess number, size, and weight of Artemia larvae. Treatment with Rhabdonema sp. showed larvae with a lower percentage of polyunsaturated fatty acids (PUFAs while Grammatophora sp. showed those with the greatest PUFA proportion, even more than those fed Chaetoceros muelleri (control. Larvae consuming Schizochytrium sp. had no docosahexanoic (DHA nor eicosapentaenoic (EPA fatty acid content. Growth and survival of A. franciscana did not show significant differences among feed treatments, except when it was fed Nitzschia sp., showing lower survival and dry weight. Treatment based on Schizochytrium sp. and Rhabdonema sp. had a greater A. franciscana size but reduced dry weight; additional tests including two or more algal species for every treatment should be carried out to determine the best yield.

  8. Risk associated with toxic blooms of marine phytoplankton functional groups on Artemia franciscana

    Directory of Open Access Journals (Sweden)

    Ana D’ors

    2014-08-01

    Full Text Available Objective: To study mortality of copepod Artemia franciscana against the occurrence of harmful marine algae and possible toxicological changes exhibited by binary and tertiary combinations of these harmful algae toxins. Methods: Tweenty four hours acute toxicity assays were performed with selected concentrations of Alexandrium minutum, Prorocentrum lima and Nitzschia N1c1 living cells. Additionally, the results were analyzed using the median-effect/combination index (CI-isobologram equation to assess possible changes in the toxic effect induced by phytoplankton functional groups. Results: Biotoxin equivalent values obtained by immunodetection were (2.12±0.10, (8.60±1.30 and (4.32±1.67 pg/cell for saxitoxin, okadaic acid and domoic acid, respectively. The 24-h LC50 values estimated to saxitoxin and okadaic acid equivalents were 4.06 and 6.27 µg/L, significantly below the value obtained for Nitzschia N1c1, which was established at 467.33 µg/L. CI analysis applied on phytoplankton assemblages showed that both ternary mixture as the binary combinations exhibited antagonic action on toxic effects in Artemia nauplii, which were significantly lower than the toxic effect exhibited by each species studied. Conclusions: These results show that, although these harmful algae represent a serious risk to estuarine zooplankton community, the presence of phytoplankton functional groups within the same bloom can reduce the potential risk compared to the expected risk when each of the phytoplankton groups are evaluated individually.

  9. A gene catalogue for post-diapause development of an anhydrobiotic arthropod Artemia franciscana

    Directory of Open Access Journals (Sweden)

    Yu Jun

    2009-01-01

    Full Text Available Abstract Background Diapause is a reversible state of developmental suspension and found among diverse taxa, from plants to animals, including marsupials and some other mammals. Although previous work has accumulated ample data, the molecular mechanism underlying diapause and reactivation from it remain elusive. Results Using Artemia franciscana, a model organism to study the development of post-diapause embryos in Arthropod, we sequenced random clones up to a total of 28,039 ESTs from four cDNA libraries made from dehydrated cysts and three time points after rehydration/reactivation, which were assembled into 8,018 unigene clusters. We identified 324 differentially-expressed genes (DEGs, P Conclusion We found that the first 5-hour period after rehydration is most important for embryonic reactivation of Artemia. As the total number of expressed genes increases significantly, the majority of DEGs were also identified in this period, including a group of water-deficient-induced genes. A group of genes with similar functions have been described in plant seeds; for instance, one of the novel LEA members shares ~70% amino-acid identity with an Arabidopsis EM (embryonic abundant protein, the closest animal relative to plant LEA families identified thus far. Our findings also suggested that not only nutrition, but also mRNAs are produced and stored during cyst formation to support rapid development after reactivation.

  10. Isolation of TDA-producing Phaeobacter strains from sea bass larval rearing units and their probiotic effect against pathogenic Vibrio spp. in Artemia cultures

    DEFF Research Database (Denmark)

    Grotkjær, Torben; Bentzon-Tilia, Mikkel; D'Alvise, Paul

    2016-01-01

    V. harveyi, which is the major bacterial pathogen in crustaceans and Mediterranean sea bass larvae cultures. Concomitantly, they significantly improved survival of V. harveyi-infected brine shrimp. 16S rRNA gene sequence homology identified the antagonists as Phaeobacter sp., and in silico DNA...... Vibrio anguillarum and reduce mortality in V. anguillarum-infected cod and turbot larvae. In this study, it was demonstrated that antagonistic Roseobacter-clade bacteria could be isolated from sea bass larval rearing units. In addition, it was shown that they not only antagonized V. anguillarum but also......-producing Phaeobacter isolated from Mediterranean marine larviculture are promising probiotic bacteria against pathogenic Vibrio in crustacean live-feed cultures for marine fish larvae....

  11. Domal salt brine migration experiments at Avery Island, Louisiana

    International Nuclear Information System (INIS)

    Krause, W.B.; Gnirk, P.F.

    1981-01-01

    Three in-situ brine migration experiments were performed in domal salt in the Avery Island mine located in southwestern Louisiana. The primary measurements included temperature, moisture collection, and pre- and post-test permeability at the experimental sites. Experimental data are discussed and compared with calculations based on the single-crystal brine migration theory. Comparisons indicate reasonable agreement between experiment and theory

  12. Intentional introduction of Artemia sinica (Anostraca) in the high-altitude Tibetan lake Dangxiong Co: the new population and consequences for the environment and for humans

    Science.gov (United States)

    Jia, Qinxian; Anufriieva, Elena; Liu, Xifang; Kong, Fanjing; Shadrin, Nickolai

    2015-11-01

    The imbalance between supply and demand of Artemia cysts in China and around the world is increasing now. Salt lakes in Tibet may contribute to the solution of the problem. In Northern Tibet there are 26 saline lakes whose salinity and temperature may support Artemia survival at an altitude of 4 000-5 100 m. We found Artemia in 15 of these lakes. The saline lakes with Artemia populations mainly belong to the shallow basin lakes, and the majority of these lakes are small in area. The total area of lakes without Artemia is more than 1 000 km2. Lake Dangxiong Co (Co means lake in Tibet) was chosen for the intentional introduction of Artemia sinica. In 2004, 850 g of A. sinica cysts, originating from Qinghai, were introduced in the lake. Surveys in 2006-2014 showed that the average abundance of Artemia adults in the lake gradually increased from 20 ind./m3 in 2006 to 1950 ind./m3 in 2013. We assume that two subpopulations of A. sinica, separated by depth, may exist in the lake. The new Artemia population caused an increase in the number of species of phytoplankton and heterotrophic protozoa with a decrease of their total abundance. Water transparency also increased. Dominance in phytoplankton passed from cyanobacteria to diatoms. Changes occurred not only in the lake ecosystem; the number of water birds using the lakes also dramatically increased. Preliminary calculations showed that is it possible to harvest at least about 150 t cysts per year from the lake as well as 3.2 thousand tons of frozen or 350 t of dried biomass of adult Artemia.

  13. The history, present status and prospects of the availability of Artemia cysts for aquaculture

    OpenAIRE

    Lavens, P.; Sorgeloos, P.

    2000-01-01

    This paper provides an overview of the history of Artemia cyst provision worldwide since the 1950s. It allows a better assessment of the current situation, characterized by poor yields from the main harvest site, the Great Salt Lake in Utah, USA, and to make prognoses about future supplies and demands.

  14. Successful Replacement of Artemia salina nauplii with Marine Rotifers (Brachionus plicatilis) in the Diet of Preadult Zebrafish (Danio rerio).

    Science.gov (United States)

    Lawrence, Christian; James, Althea; Mobley, Scottie

    2015-10-01

    Previously established rearing protocols for zebrafish begin feeding with marine rotifers (Brachionus plicatilis), followed by Artemia nauplii until the fish reach subadult stage, the developmental time point at which they can be most easily transitioned onto a processed diet. However, the inclusion of Artemia is less than ideal, given its fluctuating availability and high costs. We tested whether or not we could replace Artemia with rotifers during our normal rearing sequence and still meet published performance standards for (i) weaning fish onto a processed diet by 25 days postfertilization (dpf) and (ii) successful breeding by 60 dpf. Here, we present the results of trials where wild-type and casper zebrafish were fed exclusively with rotifers (R) or rotifers followed by Artemia (RA) for the first 25 dpf after which point all fish were transitioned to a processed diet (Gemma Micro 300). We measured growth and survival at days 25 and 60, and tested for reproductive capability at 60 dpf. While growth performance was significantly better in the RA groups, we were still able to meet goals for both weaning and generation time in the R groups without compromising survival or sex ratios.

  15. Population structure and recruitment of penaeid shrimps in ...

    African Journals Online (AJOL)

    Population structure and recruitment of penaeid shrimps in mozambique. ... This study characterizes the population structure and identifies nursery areas and recruitment seasons of penaeid shrimps in the ... AJOL African Journals Online.

  16. Enzyme-assisted peeling of cold water shrimps (Pandalus borealis)

    DEFF Research Database (Denmark)

    Dang, Tem Thi; Gringer, Nina; Jessen, Flemming

    2018-01-01

    An enzymatic method to facilitate the peeling of cold water shrimps (Pandalus borealis) was developed. The protease solutions were used to mature the shrimps to promote shell-loosening prior to peeling. The efficiency of peeling enzyme-treated shrimps was evaluated by a new quantitative measurement......L and 0.25% Exocut-A0 for 20 h resulted in the best peeling of shrimps (100% completely peeled shrimps, 3 mJ/g work and 89% meat yield). Reuse of the enzyme solution was possible due to a 95% retention rate of proteolytic activity after two 20-h cycles of maturation. The studied enzymatic maturation...... of shrimp. This approach would benefit the shrimp processing industry by 1) enhancing peeling efficiency that includes least efforts to remove the shell, high rate of completely peeled shrimps and high meat yield; 2) shortening the duration of maturation but still sufficiently loosening the shell...

  17. 2012 Economic Survey of Gulf State Shrimp License Holders

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This mail survey collected economic data on inshore commercial shrimp fishermen who held licenses to commercially harvest shrimp in state waters of the U.S. Gulf of...

  18. White spot viral disease in penaeid shrimp-a review

    OpenAIRE

    Sangamaheswaran, A.P.; Jeyaseelan, M.J.P.

    2001-01-01

    The white spot viral disease in penaeid shrimp affects the development of the global shrimp industry. This paper reviews the viruses that cause the disease, the transmission of the virus, diagnosis and preventive measures.

  19. Penaeid Shrimp Salinity Gradient Tank Study 2005-2008

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — We designed an experimental gradient tank to examine salinity preferences of juvenile brown shrimp and white shrimp. Although no strong pattern of salinity avoidance...

  20. Gamma Irradiation for the Inhibition of Shrimp (Penaeus aztecus) Allergy

    International Nuclear Information System (INIS)

    Kim Jae-Hun; Lee Ju-Woon

    2000-01-01

    Food irradiation technology was conducted to reduce shrimp allergy. The experiment was designated in 3 portions as follows; A, the irradiation of raw shrimp; B the irradiation of shrimp and then cooking; and C, cooking the shrimp and then irradiation. Gamma irradiation was done with doses of 1, 3, 5, 7, 10 kGy. A shrimp sarcoplasmic protein solution (SSPS) and a myofibrillar protein solution (SMPS) were prepared from A portion. Cooked shrimp protein solutions were also prepared from B and C portions. The binding abilities of the shrimp allergic patients' IgE and mouse monoclonal Ab 4.9.5 (mAb 4.9.5), produced to the shrimp heat-stable protein, to each sample solution were determined by ELISA. Binding abilities of patients' IgE and mAb 4.9.5 to irradiated shrimp fractions were dose-dependently reduced. The cooking treatment after irradiation was more effective than the irradiation treatment after cooking in the reduction of the binding abilities of IgE and IgG. SDS-PAGE was performed to compare irradiated shrimp proteins with non-irradiated shrimp proteins. SDS-PAGE showed that no bands were changed by gamma irradiation. The results indicated that food irradiation with an adequate dose can be reduce allergenicity of shrimp

  1. Intestinal bacterial signatures of white feces syndrome in shrimp.

    Science.gov (United States)

    Hou, Dongwei; Huang, Zhijian; Zeng, Shenzheng; Liu, Jian; Wei, Dongdong; Deng, Xisha; Weng, Shaoping; Yan, Qingyun; He, Jianguo

    2018-04-01

    Increasing evidence suggests that the intestinal microbiota is closely correlated with the host's health status. Thus, a serious disturbance that disrupts the stability of the intestinal microecosystem could cause host disease. Shrimps are one of the most important products among fishery trading commodities. However, digestive system diseases, such as white feces syndrome (WFS), frequently occur in shrimp culture and have led to enormous economic losses across the world. The WFS occurrences are unclear. Here, we compared intestinal bacterial communities of WFS shrimp and healthy shrimp. Intestinal bacterial communities of WFS shrimp exhibited less diversity but were more heterogeneous than those of healthy shrimp. The intestinal bacterial communities were significantly different between WFS shrimp and healthy shrimp; compared with healthy shrimp, in WFS shrimp, Candidatus Bacilloplasma and Phascolarctobacterium were overrepresented, whereas Paracoccus and Lactococcus were underrepresented. PICRUSt functional predictions indicated that the relative abundances of genes involved in energy metabolism and genetic information processing were significantly greater in WFS shrimp. Collectively, we found that the composition and predicted functions of the intestinal bacterial community were markedly shifted by WFS. Significant increases in Candidatus Bacilloplasma and Phascolarctobacterium and decreases in Paracoccus and Lactococcus may contribute to WFS in shrimp.

  2. The efficacy testing of irradiated shrimp paste

    International Nuclear Information System (INIS)

    Nouchpramool, Kovit; Eamsiri, Jaruratana; Sujjabut, Surusak

    2005-10-01

    Two lots of shrimp paste from commercial source in Samutsakhon were irradiated at a recommended minimum dose of 6 kGy using a J S 8900 cobalt-60 carrier gamma irradiator of Thai Irradiation Center in Patum Thani. Red Perspex dosimeter were used to measure the absorbed dose throughout the product with emphasis on the region of minimum and maximum absorbed dose. This way, it was aimed to compare the dose effects of gamma irradiation on the microbiological, chemical and sensory quality of shrimp paste. The results indicated that the shrimp paste received minimum and maximum absorbed dose of 6.85 and 12.83 kGy with dose uniformity ratio of 1.87 . Throughput rate is 468 kilogram per hour. The microbiological load of shrimp paste was rather high resulting in not compliance with Thai industrial standard 1080-2535. Irradiation at 6.8 kGy reduced total viable bacterial count by one log cycle. Although the irradiated product was organoleptic ally acceptable and could be kept for 16 months at room temperature, mold and Clostridium perfringens were still present in some samples after irradiation and during prolonged storage in amount that exceeds the limitation of Thai industrial standard. Chemical properties such as p H, moisture and sodium chloride content of irradiated shrimp paste were not significantly changed after irradiation

  3. Novel mesostructured inclusions in the epidermal lining of Artemia franciscana ovisacs show optical activity

    Directory of Open Access Journals (Sweden)

    Elena Hollergschwandtner

    2017-10-01

    Full Text Available Background Biomineralization, e.g., in sea urchins or mollusks, includes the assembly of mesoscopic superstructures from inorganic crystalline components and biopolymers. The resulting mesocrystals inspire biophysicists and material scientists alike, because of their extraordinary physical properties. Current efforts to replicate mesocrystal synthesis in vitro require understanding the principles of their self-assembly in vivo. One question, not addressed so far, is whether intracellular crystals of proteins can assemble with biopolymers into functional mesocrystal-like structures. During our electron microscopy studies into Artemia franciscana (Crustacea: Branchiopoda, we found initial evidence of such proteinaceous mesostructures. Results EM preparations with high-pressure freezing and accelerated freeze substitution revealed an extraordinary intracellular source of mesostructured inclusions in both the cyto-and nucleoplasm of the epidermal lining of ovisacs of A. franciscana. Confocal reflection microscopy not only confirmed our finding; it also revealed reflective, light dispersing activity of these flake-like structures, their positioning and orientation with respect to the ovisac inside. Both the striation of alternating electron dense and electron-lucent components and the sharp edges of the flakes indicate self-assembly of material of yet unknown origin under supposed participation of crystallization. However, selected area electron diffraction could not verify the status of crystallization. Energy dispersive X-ray analysis measured a marked increase in nitrogen within the flake-like inclusion, and the almost complete absence of elements that are typically involved in inorganic crystallization. This rise in nitrogen could possibility be related to higher package density of proteins, achieved by mesostructure assembly. Conclusions The ovisac lining of A. franciscana is endowed with numerous mesostructured inclusions that have not been

  4. Balancing options for shrimp farming : a landscape approach to investigate the future of shrimp farming in the Mekong Delta

    NARCIS (Netherlands)

    Joffre, O.M.

    2015-01-01

    Balancing options for shrimp farming

    A landscape approach to investigate the future of shrimp farming in the Mekong Delta

    Olivier Joffre

    While providing an option for development in coastal areas, shrimp farming is usually associated with high environmental

  5. Mantle helium in the Red Sea brines

    International Nuclear Information System (INIS)

    Lupton, J.E.; Weiss, R.F.; Craig, H.

    1977-01-01

    It is stated that He isotope studies of terrestrial samples have shown the existence of two He components that are clearly distinct from atmospheric He. These are termed 'crustal' He and 'mantle' He; the latter was discovered as 'excess 3 He' in deep ocean water and attributed to a flux of primordial He from the mantle. Studies of the 3 He/ 4 He ratio in deep Pacific water and in He trapped in submarine basalt glasses showed that this 'mantle' component is characterised by ratios about ten times the atmospheric ratio and 100 times the ratio in 'crustal' He. Basalt glasses from other deep sea waters also showed similar ratios, and it is indicated that 'mantle' He in areas in which new lithosphere is being formed has a unique and uniform isotopic signature. Measurements of He and Ne are here reported that reveal additional information on the origin of Red Sea brines and their relationship to the Red Sea rifts. (U.K.)

  6. Radionuclide transport in sandstones with WIPP brine

    International Nuclear Information System (INIS)

    Weed, H.C.; Bazan, F.; Fontanilla, J.; Garrison, J.; Rego, J.; Winslow, A.M.

    1981-02-01

    Retardation factors (R) have been measured for the transport of 3 H, /sup 95m/Tc, and 85 Sr in WIPP brine using St. Peter, Berea, Kayenta, and San Felipe sandstone cores. If tritium is assumed to have R=1, /sup 95m/Tc has R=1.0 to 1.3 and therefore is essentially not retarded. Strontium-85 has R = 1.0 to 1.3 on St. Peter, Berea, and Kayenta, but R=3 on San Felipe. This is attributed to sorption on the matrix material of San Felipe, which has 45 volume % matrix compared with 1 to 10 volume % for the others. Retardation factors (R/sub s/) for 85 Sr calculated from static sorption measurements are unity for all the sandstones. Therefore, the static and transport results for 85 Sr disagree in the case of San Felipe, but agree for St. Peter, Berea, and Kayenta

  7. Evaporation Rates of Brine on Mars

    Science.gov (United States)

    Sears, D. W. G.; Chittenden, J.; Moore, S. R.; Meier, A.; Kareev, M.; Farmer, C. B.

    2004-01-01

    While Mars is now largely a dry and barren place, recent data have indicated that water has flowed at specific locations within the last approx. 10(exp 6) y. This had led to a resurgence of interest in theoretical and experimental work aimed at understanding the behavior of water on Mars. There are several means whereby the stability of liquid water on Mars could be increased, one being the presence solutes that would depress the freezing point. Salt water on Earth is about 0.5M NaCl, but laboratory experiments suggest that martian salt water is quite different. We recently began a program of laboratory measurements of the stability of liquid water, ice and ice-dust mixtures under martian conditions and here report measurements of the evaporation rate of 0.25M brine.

  8. Brine migration in hot-pressed polycrystalline sodium chloride

    International Nuclear Information System (INIS)

    Biggers, J.V.; Dayton, G.O.

    1982-12-01

    This report describes experiments designed to provide data on brine migration in polycrystalline salt. Polycrystalling samples of various grain sizes, density, and purity were prepared from several commercial-grade salts by hot-pressing. Three distinct experimental set-ups were used to place salt billets in an induced thermal gradient in contact with brine source. The test designs varied primarily in the way in which the thermal gradient was applied and monitored and the way in which brine migration was determined. All migration was in enclosed vessels which precluded visual observation of brine movement through the microstructure. Migration velocities were estimated either by the timed appearance of brine at the hot face of the sample, or by determination of the penetration distance of migration artifacts in the microstructure after tests of fixed duration. For various reasons both of these methods were subject to a large degree of error. Our results suggest, however, that the migration velocity in dense polycrystalline salt may be at least an order of magnitude greater than that suggested by single-crystal experiments. Microstructural analysis shows that brine prefers to migrate along paths of high crystalline activity such as grain and subgrain boundaries and is dispersed rather quickly in the microstructure. A series of tests were performed using various types of tracers in brine in order to flag migration paths and locate brine in the microstructure more decisively. These attempts failed and it appears that only the aqueous portion of the brine moves through the microstructure with the dissolved ions being lost and replaced rather quickly. This suggests the use of deuterium as a tracer in future work

  9. First secondary metabolites from Herissantia crispa L (Brizicky) and the toxicity activity against Artemia salina Leach

    Energy Technology Data Exchange (ETDEWEB)

    Costa, Danielly Albuquerque da; Matias, Wemerson Neves; Lima, Igara Oliveira; Xavier, Aline Lira; Costa, Vivian Bruna Machado; Diniz, Margareth de Fatima Formiga Melo; Agra, Maria de Fatima; Batista, Leonia Maria; Souza, Maria de Fatima Vanderlei de [Universidade Federal da Paraiba (UFPB), Joao Pessoa, PB (Brazil). Lab. de Tecnologia Farmaceutica Prof. Delby Fernandes de Medeiros; Silva, Davi Antas e [Universidade Federal Rural de Pernambuco, Serra Talhada, PE (Brazil). Dept. de Quimica

    2009-07-01

    The phytochemical investigation of Herissantia crispa led to the isolation of seven compounds, identified as: sitosterol 3-O-{beta}-D-glucopyranoside, stigmasterol 3-O-{beta}-D-glucopyranoside, 3,5,7,4'-tetrahydroxyflavone (kaempferol), 3,5,7,3',4'-pentahydroxyflavone (quercetin), unpublished in the genus Herissantia, besides {beta}-sitosterol, kaempferol 3-O-{beta}-D-(6''-E-p-coumaroil) (tiliroside) glucopyranoside and kaempferol 3,7-di-O-{alpha}-L-ramnopyranoside (lespedin), described for the first time in the species. The structural determination of the compounds was made by means of spectroscopy methods such as Infrared Spectroscopy, {sup 1}H and {sup 13}C Nuclear Magnetic Resonance, with the aid of two dimensional techniques, and by comparison with literature data. The toxicity activity of the MeOH extract and lespedin on Artemia salina Leach. was also carried out. (author)

  10. Interaction of Low Doses of Ionizing Radiation, Potassium Dichromate and Cadmium Chloride in Artemia franciscana Biotest

    Directory of Open Access Journals (Sweden)

    K. Beňová

    2007-01-01

    Full Text Available The influence of cadmium chloride (at concentrations of 100 and 200 mg l-1 and potassium dichromate (at a concentration of 50 mg l-1 along with the effect of gamma radiation 60Co (at a dose of 10 and 50 Gy on lethality to Artemia franciscana was investigated. Four different interactions were studied, namely, those of potassium dichromate and gamma radiation, cadmium chloride and gamma radiation, and combinations of potassium dichromate and cadmium chloride in interaction with gamma radiation. A significant (α = 0.05 decrease was observed in lethality due to exposure to radiation (10 Gy in comparison with action of only potassium dichromate and cadmium chloride or their combination without exposure to gamma rays. These results support the theory of hormesis.

  11. Oxygen-rich hierarchical porous carbon derived from artemia cyst shells with superior electrochemical performance.

    Science.gov (United States)

    Zhao, Yufeng; Ran, Wei; He, Jing; Song, Yanfang; Zhang, Chunming; Xiong, Ding-Bang; Gao, Faming; Wu, Jinsong; Xia, Yongyao

    2015-01-21

    In this study, three-dimensional (3D) hierarchical porous carbon with abundant functional groups is produced through a very simple low-cost carbonization of Artemia cyst shells. The unique hierarchical porous structure of this material, combining large numbers of micropores and macropores, as well as reasonable amount of mesopores, is proven favorable to capacitive behavior. The abundant oxygen functional groups from the natural carbon precursor contribute stable pseudocapacitance. As-prepared sample exhibits high specific capacitance (369 F g(-1) in 1 M H2SO4 and 349 F g(-1) in 6 M KOH), excellent cycling stability with capacitance retention of 100% over 10 000 cycles, and promising rate performance. This work not only describes a simple way to produce high-performance carbon electrode materials for practical application, but also inspires an idea for future structure design of porous carbon.

  12. First secondary metabolites from Herissantia crispa L (Brizicky) and the toxicity activity against Artemia salina Leach

    International Nuclear Information System (INIS)

    Costa, Danielly Albuquerque da; Matias, Wemerson Neves; Lima, Igara Oliveira; Xavier, Aline Lira; Costa, Vivian Bruna Machado; Diniz, Margareth de Fatima Formiga Melo; Agra, Maria de Fatima; Batista, Leonia Maria; Souza, Maria de Fatima Vanderlei de; Silva, Davi Antas e

    2009-01-01

    The phytochemical investigation of Herissantia crispa led to the isolation of seven compounds, identified as: sitosterol 3-O-β-D-glucopyranoside, stigmasterol 3-O-β-D-glucopyranoside, 3,5,7,4'-tetrahydroxyflavone (kaempferol), 3,5,7,3',4'-pentahydroxyflavone (quercetin), unpublished in the genus Herissantia, besides β-sitosterol, kaempferol 3-O-β-D-(6''-E-p-coumaroil) (tiliroside) glucopyranoside and kaempferol 3,7-di-O-α-L-ramnopyranoside (lespedin), described for the first time in the species. The structural determination of the compounds was made by means of spectroscopy methods such as Infrared Spectroscopy, 1 H and 13 C Nuclear Magnetic Resonance, with the aid of two dimensional techniques, and by comparison with literature data. The toxicity activity of the MeOH extract and lespedin on Artemia salina Leach. was also carried out. (author)

  13. Determination of Biogenic Amines in Different Shrimp Species for Export

    International Nuclear Information System (INIS)

    Myat Myat Thaw; Oo Aung; Aung Myint; Bisswanger, Hans

    2004-06-01

    This study is part of the project on the ''Quality Assurance of Different Shrimp Species for Export''. Local shrimp samples were collected from Ministry of Livestock and Fisheries and various private enterprises. Contents of biogenic amines were determined by using benzoyl chloride derivatization method with HPLC (reverse phase high performance liquid chromatography). Based on the biogenic amines, quality index of shrimps were correlated with freshness index so that the grade of shrimp samples can be classified as excellent, good, and acceptable. All sizes of shrimps such as extra large, large, medium were found to excceptable respectively

  14. Involvement of cyclin K posttranscriptional regulation in the formation of Artemia diapause cysts.

    Directory of Open Access Journals (Sweden)

    Yang Zhao

    Full Text Available BACKGROUND: Artemia eggs tend to develop ovoviviparously to yield nauplius larvae in good rearing conditions; while under adverse situations, they tend to develop oviparously and encysted diapause embryos are formed instead. However, the intrinsic mechanisms regulating this process are not well understood. PRINCIPAL FINDING: This study has characterized the function of cyclin K, a regulatory subunit of the positive transcription elongation factor b (P-TEFb in the two different developmental pathways of Artemia. In the diapause-destined embryo, Western blots showed that the cyclin K protein was down-regulated as the embryo entered dormancy and reverted to relatively high levels of expression once development resumed, consistent with the fluctuations in phosphorylation of position 2 serines (Ser2 in the C-terminal domain (CTD of the largest subunit (Rpb1 of RNA polymerase II (RNAP II. Interestingly, the cyclin K transcript levels remained constant during this process. In vitro translation data indicated that the template activity of cyclin K mRNA stored in the postdiapause cyst was repressed. In addition, in vivo knockdown of cyclin K in developing embryos by RNA interference eliminated phosphorylation of the CTD Ser2 of RNAP II and induced apoptosis by inhibiting the extracellular signal-regulated kinase (ERK survival signaling pathway. CONCLUSIONS/SIGNIFICANCE: Taken together, these findings reveal a role for cyclin K in regulating RNAP II activity during diapause embryo development, which involves the post-transcriptional regulation of cyclin K. In addition, a further role was identified for cyclin K in regulating the control of cell survival during embryogenesis through ERK signaling pathways.

  15. Feeding adult of Artemia salina (Crustacea-Branchiopoda on the dinoflagellate Gyrodinium corsicum (Gymnodiniales and the Chryptophyta Rhodomonas baltica

    Directory of Open Access Journals (Sweden)

    Rauquírio André Albuquerque Marinho da Costa

    2005-07-01

    Full Text Available Experiments were carried out on feeding performance and survival rates of adult Artemia salina exposed to no axenic strains of the dinoflagellate Gyrodinium corsicum and of the Chryptophyta Rhodomonas baltica. Filtration rates on R. baltica and G. corsicum varied from 3.35 to 7.14 ml.artemia-1.h-1 and from 2.97 to 15.86 ml.artemia-1.h-1, respectively. The ingestion rates observed for A. salina did not indicate any digestive dysfunction or physiological impairment for organisms fed on G. corsicum and their functional response were similar to those observed for other organisms like copepod fed on different food concentrations. Mortality rates oscillated from 2.5% to 100% when A. salina was fed on R. baltica or G. corsicum, respectively. Highest mortality rates observed for organisms fed on G. corsicum indicated that this dinoflagellate presented a hazard effect on A. salina that was not possible to confirm if it was related to toxin production or to nutritive inadequacy of this dinoflagellate as food for organisms of this species.Experimentos foram desenvolvidos para estudar as taxas de alimentação e de sobrevivência de Artemia salina alimentada com cepas não tóxicas do dinoflagelado Gyrodinium corsicum e da Chryptophyta Rhodomonas baltica. As taxas de filtração sobre R. baltica e G. corsicum variaram entre 3,35 e 7,14 ml.artemia-1.h-1 e 2,97 e 15,86 ml.artemia-1.h-, respectivamente. As taxas de ingestão observadas para A. salina não indicaram disfunção digestiva ou prejuízo fisiológico nos organismos alimentados com G. corsicum, sendo a resposta funcional destes organismos similar a observada em copépodos alimentados com diferentes concentrações de alimento. As taxas de mortalidade de A. salina oscilaram entre 2,5 e 100% quando alimentada com R. baltica e G. corsicum, respectivamente. As maiores taxas de mortalidade observadas para os organismos alimentados com G. corsicum indicam que este dinoflagelado apresenta algum efeito

  16. 75 FR 49460 - Administrative Review of Certain Frozen Warmwater Shrimp From the People's Republic of China...

    Science.gov (United States)

    2010-08-13

    ... the surrogate values for shrimp larvae, diesel fuel, shrimp waste, and by- products. For further... Korean Import Data Comment 3: Shrimp Larvae Comment 4: Shrimp Feed Comment 5: Electricity Comment 6... Certain Frozen Warmwater Shrimp From the People's Republic of China: Final Results and Partial Rescission...

  17. Modelling CO2-Brine Interfacial Tension using Density Gradient Theory

    KAUST Repository

    Ruslan, Mohd Fuad Anwari Che

    2018-01-01

    In this study, a new modelling strategy to compute CO2-brine IFT based on DGT was proposed. In the proposed model, ion distribution across interface was accounted for by separating the interface to two sections

  18. Effect of different brine concentrations and ripening period on some ...

    African Journals Online (AJOL)

    Administrator

    2011-09-26

    25240, Erzurum, Turkey. Accepted 25 ... ripened soft cheese that is maturated in brine to develop the desired ... functions, salt exerts a number of important effects on cheese. ..... In: Fox PF (ed) Cheese: chemistry, physics and.

  19. determination of toxicity levels of some savannah plants using brine

    African Journals Online (AJOL)

    DR. AMINU

    DETERMINATION OF TOXICITY LEVELS OF SOME SAVANNAH PLANTS. USING BRINE ... Adoum, O. A.. Department of Pure and Industrial Chemistry, Bayero University, P.M.B. 3011, Kano – Nigeria. ... 1000, 100, and 10 µg/ml, respectively.

  20. A network model for characterizing brine channels in sea ice

    Science.gov (United States)

    Lieblappen, Ross M.; Kumar, Deip D.; Pauls, Scott D.; Obbard, Rachel W.

    2018-03-01

    The brine pore space in sea ice can form complex connected structures whose geometry is critical in the governance of important physical transport processes between the ocean, sea ice, and surface. Recent advances in three-dimensional imaging using X-ray micro-computed tomography have enabled the visualization and quantification of the brine network morphology and variability. Using imaging of first-year sea ice samples at in situ temperatures, we create a new mathematical network model to characterize the topology and connectivity of the brine channels. This model provides a statistical framework where we can characterize the pore networks via two parameters, depth and temperature, for use in dynamical sea ice models. Our approach advances the quantification of brine connectivity in sea ice, which can help investigations of bulk physical properties, such as fluid permeability, that are key in both global and regional sea ice models.

  1. Coiled Brine Recovery Assembly (CoBRA) Project

    Data.gov (United States)

    National Aeronautics and Space Administration — The Coiled Brine Recovery Assembly (CoBRA) project will result in a proof-of-concept demonstration for a lightweight, compact, affordable, regenerable and disposable...

  2. Brine treatment, smoking and storage techniques: their effects on the ...

    African Journals Online (AJOL)

    Journal of Food Technology in Africa ... Brine treatment, smoking and storage techniques: their effects on the microbial quality of smoked mackerel. ... off odour development, softening of the fish and positively affected personal preference.

  3. Monitoring of Miit glass solution interactions by brine analysis

    International Nuclear Information System (INIS)

    Sassoon, R.E.; Gong, M.; Adel-Hadadi, M.; Brandys, M.; Barkatt, A.; Macedo, P.B.

    1989-01-01

    Analyses of brine samples taken from borehole MIIT=8 at the WIPP site were carried out in order to study the leaching behavior of the brine in this system. The standard addition method was used with the analytical techniques of AA, DCP and ICP-MS to determine the concentration of the components in the brine. The changes in the concentration of the major components, Na, Mg and K can be explained by reactions of the brine with the rock salt walls of the borehole. From the data obtained for the other components no leaching of the SRL-Y glass discs in the test could be observed. It was however possible to determine an upper limit for leaching of the glass from isotope ratio studies made on Li which yielded a value for the leach rate of lithium from the glass of 0.117 g m -2 d -1

  4. Prospecção fitoquímica de Sonchus oleraceus e sua toxicidade sobre o microcrustáceo Artemia salina Phytochemical prospecting of Sonchus oleraceus and its toxicity to Artemia salina

    Directory of Open Access Journals (Sweden)

    J.M. Lima

    2009-03-01

    Full Text Available A espécie vegetal Sonchus oleraceus é uma planta daninha presente em diversas culturas no Brasil e de utilização na medicina popular. Neste trabalho, realizou-se a prospecção fitoquímica dessa espécie com extratos em etanol, água e diclorometano, bem como testes de toxicidade sobre o microcrustáceo Artemia salina. O extrato aquoso apresentou em sua composição açúcares redutores, compostos fenólicos, taninos, flavonóides e cumarinas. No extrato etanólico, observaram-se os mesmos compostos qualificados no extrato aquoso, com exceção de cumarinas. Em diclorometano, verificou se a presença de saponinas, derivados triterpênicos e esteróides. No teste de toxicidade sobre Artemia salina, os dados convergiram para frações de extrato aquoso de 5.117,2 ppm, indicando ser um extrato de baixa toxicidade.Sonchus oleraceus is a common weed in Brazil, also used as a medicinal plant. Phytochemical prospecting of this species was carried out in this work using extracts obtained in ethanol, water and dichloromethane. A toxicity study of the aqueous extract was also conducted, using the micro crustaceous Artemia salina. The aqueous extract presented sugar reducers, phenolic compounds, tannins, flavonoids and coumarins in its composition. The compounds found in the aqueous extract were also detected in the ethanol extract, except for the coumarins. Saponin, triterpenes and steroids were identified in the dichloromethane extract. The toxicity test on Artemia salina pointed to aqueous extract fractions of 5,117.2 ppm, indicative of low toxicity.

  5. Physiological characteristics of bacteria isolated from water brines within permafrost

    Science.gov (United States)

    Shcherbakova, V.; Rivkina, E.; Laurinavichuis, K.; Pecheritsina, S.; Gilichinsky, D.

    2004-01-01

    In the Arctic there are lenses of overcooled water brines (cryopegs) sandwiched within permafrost marine sediments 100 120 thousand years old. We have investigated the physiological properties of the pure cultures of anaerobic Clostridium sp. strain 14D1 and two strains of aerobic bacteria Psychrobacter sp. isolated from these cryopegs. The structural and physiological characteristics of new bacteria from water brines have shown their ability to survive and develop under harsh conditions, such as subzero temperatures and high salinity.

  6. Calcium extraction from brine water and seawater using oxalic acid

    Science.gov (United States)

    Natasha, Nadia Chrisayu; Lalasari, Latifa Hanum

    2017-01-01

    Calcium can be extracted not only from rocks but also from natural liquor such as seawater and brine water. In order to extract the calcium from seawater and brine water, oxalic acid was used in this research. Effect of variations of the volume of the oxalic acid at a constant concentration in seawater and brine water to produce calcium was investigated. The concentration of oxalic acid was 100 g/l and the variations of its volume were 2 ml, 4 ml, 6 ml, 8 ml, 10 ml, 20 ml, 30 ml, 40 ml, and 50 ml. The used seawater and brine water were firstly evaporated from 100 ml into 50 ml and then the oxalic acid was added into them with mixing to produce the calcium precipitates. The precipitates were analyzed by X-ray diffraction (XRD) and scanning electron microscope (SEM) and the filtrates were analyzed by inductively coupled plasma-optical emission spectrometry (ICP-OES). The SEM analysis showed that the precipitates from brine water were consisted of only calcium compound while from seawater sodium one was also found along with calcium compound. The XRD analysis showed that the calcium was present in the form of calcium oxalate for both seawater and brine water. The ICP-OES analysis of the filtrate from seawater precipitation showed that the its calcium content was decreased from 826.20 ppm to 0.04 ppm while from brine water, it decreased from 170.06 ppm to 1.96 ppm. These results showed that both seawater and brine water have the potential to be a raw material for calcium production.

  7. Utilização da artêmia nacional como dieta para pós-larvas do Litopenaeus vannamei (Boone, 1931 na fase berçário = Brazilian artemia as feed for Litopenaeus vannamei post-larvae through the nursery phase

    Directory of Open Access Journals (Sweden)

    Adriano Prysthon da Silva

    2006-07-01

    Full Text Available Experimentos foram realizados em uma fazenda comercial de criação decamarões marinhos, objetivando avaliar a taxa de crescimento e sobrevivência das pós-larvas do Litopenaeus vannamei, quando cultivadas em tanques-berçário e submetidas a diferentes dietas. As pós-larvas utilizadas encontravam-se com 19 dias (PL19 e foram estocadas a 16 PL/L, em tanques-berçário de 60 m3. As pós-larvas foram submetidas ao Método de Alimentação Convencional (MAC e o Método de Alimentação com Artêmia (MAA. Verificou-se que ao se correlacionar o peso em função do comprimento e do tempo de cultivo,o MAA apresentou uma relação estatisticamente superior (pExperiments were carried out in a commercial marine shrimp farm toevaluate growth and survival rate of the Litopenaeus vannamei post-larvae during nursery phase using different diets. The nursery tanks (60 m3 will be provided with post-larvae of 19 days (PL19 with a density of 16 PL/liter. The post-larvae were submitted to a Commercial Feeding Method (MAC and an Artemia Feeding Method (MAA. Considering the correlations between weight and length, and between weight and culture duration, a better efficiency (p<0.05 of the MAA was verified. The survival rate of the post-larvae fed with MAA was 86.25%, while MAC yielded survivorship of 62.12%. Thus, artemia nauplii were proved to be more efficient, providing higher growth and survival rates of Litopenaeus vannamei post-larvae.

  8. Results on artemia cysts, lettuce and tobacco seeds in the Biobloc 4 experiment flown aboard the Soviet biosatellite Cosmos 1129

    International Nuclear Information System (INIS)

    Gaubin, Y.; Planel, H.; Gasset, G.; Pianezzi, B.

    1983-01-01

    The effects of space flight factors, in particular the heavy ion component of cosmic rays, on dormant stages of life forms were investigated as part of the Biobloc 4 experiment flown aboard the Cosmos 1129 biosatellite. Artemia cysts and seeds of tobacco and lettuce plants were placed in tubes and in monolayers sandwiched between layers of visual particle track detectors. Although Artemia cysts exposed in the dry state did not differ from ground controls, hydrated cysts exhibited a slight decrease in hatchability and reduced (C-14)O2 incorporation and protein and nucleic acid synthesis. For cysts held in the monolayers, hits by HZE particles were observed to stimulate emergence, hatching and survival. Higher proportions of chromosomal aberrations were found in lettuce seeds hit by HZE particles, while space flight produced a stimulatory effect on both germination rate and abnormality frequency in both hit and nonhit tobacco seeds. 9 references

  9. Characterization of PHB1 and its role in mitochondrial maturation and yolk platelet degradation during development of Artemia embryos.

    Directory of Open Access Journals (Sweden)

    Xiang Ye

    Full Text Available BACKGROUND: To cope with harsh environments, crustaceans such as Artemia produce diapause gastrula embryos (cysts with suppressed metabolism. Metabolism and development resume during post-diapause development, but the mechanism behind these cellular events remains largely unknown. PRINCIPAL FINDING: Our study investigated the role of prohibitin 1 (PHB1 in metabolic reinitiation during post-diapause development. We found that PHB1 was developmentally regulated via changes in phosphorylation status and localization. Results from RNA interference experiments demonstrated PHB1 to be critical for mitochondrial maturation and yolk degradation during development. In addition, PHB1 was present in yolk platelets, and it underwent ubiquitin-mediated degradation during the proteolysis of yolk protein. CONCLUSIONS/SIGNIFICANCE: PHB1 has an indispensable role in coordinating mitochondrial maturation and yolk platelet degradation during development in Artemia. This novel function of PHB1 provides new clues to comprehend the roles of PHB1 in metabolism and development.

  10. Is the Dutch shrimp fishery sustainable?

    NARCIS (Netherlands)

    Welleman, H.C.; Daan, N.

    2001-01-01

    The fishery of the brown shrimp (Crangon crangon LINNEAUS 1758) is a widespread human activity in the coastal zone. Yet management of this fishery has never been implemented. The question is raised whether an uncontrolled fishery is sustainable or the conceivable ecological stress results in

  11. CO₂ efflux from shrimp ponds in Indonesia.

    Science.gov (United States)

    Sidik, Frida; Lovelock, Catherine E

    2013-01-01

    The conversion of mangrove forest to aquaculture ponds has been increasing in recent decades. One of major concerns of this habitat loss is the release of stored 'blue' carbon from mangrove soils to the atmosphere. In this study, we assessed carbon dioxide (CO₂) efflux from soil in intensive shrimp ponds in Bali, Indonesia. We measured CO₂ efflux from the floors and walls of shrimp ponds. Rates of CO₂ efflux within shrimp ponds were 4.37 kg CO₂ m⁻² y⁻¹ from the walls and 1.60 kg CO₂ m⁻² y⁻¹ from the floors. Combining our findings with published data of aquaculture land use in Indonesia, we estimated that shrimp ponds in this region result in CO₂ emissions to the atmosphere between 5.76 and 13.95 Tg y⁻¹. The results indicate that conversion of mangrove forests to aquaculture ponds contributes to greenhouse gas emissions that are comparable to peat forest conversion to other land uses in Indonesia. Higher magnitudes of CO₂ emission may be released to atmosphere where ponds are constructed in newly cleared mangrove forests. This study indicates the need for incentives that can meet the target of aquaculture industry without expanding the converted mangrove areas, which will lead to increased CO₂ released to atmosphere.

  12. Solar Powered Automatic Shrimp Feeding System

    Directory of Open Access Journals (Sweden)

    Dindo T. Ani

    2015-12-01

    Full Text Available - Automatic system has brought many revolutions in the existing technologies. One among the technologies, which has greater developments, is the solar powered automatic shrimp feeding system. For instance, the solar power which is a renewable energy can be an alternative solution to energy crisis and basically reducing man power by using it in an automatic manner. The researchers believe an automatic shrimp feeding system may help solve problems on manual feeding operations. The project study aimed to design and develop a solar powered automatic shrimp feeding system. It specifically sought to prepare the design specifications of the project, to determine the methods of fabrication and assembly, and to test the response time of the automatic shrimp feeding system. The researchers designed and developed an automatic system which utilizes a 10 hour timer to be set in intervals preferred by the user and will undergo a continuous process. The magnetic contactor acts as a switch connected to the 10 hour timer which controls the activation or termination of electrical loads and powered by means of a solar panel outputting electrical power, and a rechargeable battery in electrical communication with the solar panel for storing the power. By undergoing through series of testing, the components of the modified system were proven functional and were operating within the desired output. It was recommended that the timer to be used should be tested to avoid malfunction and achieve the fully automatic system and that the system may be improved to handle changes in scope of the project.

  13. Neoliberalism and shrimp industry in Ecuador

    Directory of Open Access Journals (Sweden)

    Nadia Romero Salgado

    2014-04-01

    Full Text Available Analysis of the effects of the shrimp industry development in the mangrove ecosystem, the socio-environmental conflicts generated and its relationship with neoliberalism and the financial crisis of 1999 in Ecuador. After a review of the importance of the mangrove ecosystem, the stages of the shrimp expansion, its promoters, the mangrove deforestation and the socio-environmental effects caused, I will analyze the shrimp crisis, its parallels with the financial crisis of 1999 and its subsequent recovery. I will show that the shrimp industry expanded in mangrove areas in order to reduce costs, even breaking the law and creating environmental degradation, vulnerability of the costs and loss of natural resources, based on the exploitation and privatization of a public good. This created unemployment, migration and impoverishment to local populations and costs that the State will have to assume. Therefore, it is a process of “accumulation by dispossession” characteristic of neoliberalism.

  14. Cobalt-60 gamma irradiation of shrimp

    International Nuclear Information System (INIS)

    Sullivan, N.L.B.

    1993-01-01

    Meta- and ortho-tyrosine were measured using high performance liquid chromatography (HPLC) in conjunction with electrochemical detection in shrimp irradiated using cobalt-60 gamma radiation in the absorbed dose range 0.8 to 6.0 kGy, in nonirradiated shrimp, and in bovine serum albumin (BSA) irradiated in dilute aqueous solution at 25.0 kGy. Ortho-tyrosine was measured in nonirradiated BSA. Para-, meta-, and ortho-tyrosine was measured using HPLC in conjunction with uv-absorption detection in dilute aqueous solutions of phenylalanine irradiated in the absorbed dose range 16.0 to 195.0 kGy. The measured yields of tyrosine isomers were approximately linear as a function of absorbed dose in shrimp, and in irradiated solutions of phenylalanine up to 37.0 kGy. The occurrence of meta- and ortho-tyrosine, which had formerly been considered unique radiolytic products, has not previously been reported in nonirradiated shrimp or BSA. The conventional hydrolyzation and analytical techniques used in the present study to measure meta- and ortho-tyrosine may provide the basis for a method to detect and determine the dose used in food irradiation

  15. CO₂ efflux from shrimp ponds in Indonesia.

    Directory of Open Access Journals (Sweden)

    Frida Sidik

    Full Text Available The conversion of mangrove forest to aquaculture ponds has been increasing in recent decades. One of major concerns of this habitat loss is the release of stored 'blue' carbon from mangrove soils to the atmosphere. In this study, we assessed carbon dioxide (CO₂ efflux from soil in intensive shrimp ponds in Bali, Indonesia. We measured CO₂ efflux from the floors and walls of shrimp ponds. Rates of CO₂ efflux within shrimp ponds were 4.37 kg CO₂ m⁻² y⁻¹ from the walls and 1.60 kg CO₂ m⁻² y⁻¹ from the floors. Combining our findings with published data of aquaculture land use in Indonesia, we estimated that shrimp ponds in this region result in CO₂ emissions to the atmosphere between 5.76 and 13.95 Tg y⁻¹. The results indicate that conversion of mangrove forests to aquaculture ponds contributes to greenhouse gas emissions that are comparable to peat forest conversion to other land uses in Indonesia. Higher magnitudes of CO₂ emission may be released to atmosphere where ponds are constructed in newly cleared mangrove forests. This study indicates the need for incentives that can meet the target of aquaculture industry without expanding the converted mangrove areas, which will lead to increased CO₂ released to atmosphere.

  16. Thermal-gradient migration of brine inclusions in salt

    International Nuclear Information System (INIS)

    Yagnik, S.K.

    1982-02-01

    It has been proposed that the high level nuclear waste be buried deep underground in a suitable geologic formation. Natural salt deposits have been under active consideration as one of the geologic formations where a nuclear waste repository may be built in future. The salt deposits, however, are known to contain a small amount (about 0.5 vol.%) of water in the form of brine inclusions which are dispersed throughout the medium. The temperature gradients imposed by the heat generating nuclear waste will mobilize these brine inclusions. It is important to know the rate and the amount of brine accumulating at the waste packages to properly evaluate the performance of a nuclear waste repository. An extensive experimental investigation of the migration velocities of brine inclusions in synthetic single crystals of NaCl and in polycrystalline natural salt crystals has been conducted. The results show that in a salt repository the brine inclusions within a grain would move with the diffusion controlled velocities. The brine reaching a grain boundary may be swept across, if the thermal gradient is high enough. Grain boundaries in polycrystalline rock salt are apparently quite weak and open up due to drilling the hole for a waste canister and to the thermal stresses which accompany the thermal gradient produced by the heat generating waste. The enhanced porosity allows the water reaching the grain boundary to escape by a vapor transport process

  17. A successful microbound diet for the larval culture of Chinese shrimp Fenneropenaeus chinensis

    Science.gov (United States)

    Wang, Xinxia; Mai, Kangsen

    2005-07-01

    A 13-day feeding trial was carried out to evaluate the effectiveness of a microbound diet for rearing the larvae of Chinese shrimp Fenneropenaeus chinensis in comparison with the live foods that consist of Isochrysis galbana, Chlorella vulgaris, Tetraselmis chuii, rotifer (Brachionus plicatilis) and Artemia sp. Larvae of 0 to 13d post-hatch (dph) were reared in a temperature-controlled semi-open culture system and stocked at a density of 100 larvae L-1 in tanks, each containing 50 L sterilized seawater with salinity 30 32. Larvae were manually fed either the live foods or the microbound diet 6 times per day. At 13dph, the growth of the larvae fed on the microbound diet was approximately 84% of that fed on the live foods. The survival rate of the larvae fed on the microbound diet was 44.29% at 13 dph, which was not significantly different from that of larvae fed on live foods (63.55% ). The body length and development index (DI) of the larvae fed on the microbound diet were always lower than those of larvae fed on live foods. However, the differences reached significant levels only at 11 and 13dph (P<0.05). The mean dry weight loss of the microbound diet was 9.2% after 90min immersion in seawater, indicating that this diet has a good water stability. The microbound diet contains 5223% crude protein and 10.27% lipid and is easy to prepare. These characteristics of the diet suggest good potentials for its successful use in the larviculture of other penaeid and fish species.

  18. The changes in the biochemical compositions and enzymatic activities of rotifer (Brachionus plicatilis, Müller) and Artemia during the enrichment and starvation periods.

    Science.gov (United States)

    Naz, Mehmet

    2008-12-01

    The changes in the biochemical compositions and enzymatic activities of rotifer (Brachionus plicatilis) and Artemia, enriched and stored at 4 degrees C temperature, were determined. The total starvation period was 16 h and samples were taken at the end of the 8th and 16th hours. In present study, the rotifer and nauplii catabolized a large proportion of the protein during the enrichment period. Lipid contents of both live preys increased during the enrichment period and decreased in nauplii and metanauplii throughout the starvation period but lipid content of the rotifer remained relatively constant during the starvation period. The changes observed in the amino acid compositions of Artemia and the rotifer were statistically significant (P < 0.05). The conspicuous decline the essential amino acid (EAA) and nonessential amino acid (NEAA) content of the rotifer was observed during the enrichment period. However, the essential amino acid (EAA) and nonessential amino acid (NEAA) contents of Artemia nauplii increased during the enrichment period. The unenriched and enriched rotifers contained more monounsaturated fatty acid (MUFAs) than polyunsaturated fatty acid (PUFAs) and saturated fatty acids (SFA). However, Artemia contained more PUFAs than MUFAs and SFA during the experimental period. A sharp increase in the amounts of docosahexaenoic acid (DHA) during the enrichment of the rotifer and Artemia nauplii was observed. However, the amount of DHA throughout the starvation period decreased in Artemia metanauplii but not in Artemia nauplii. Significant differences in tryptic, leucine aminopeptidase N (LAP), and alkaline phosphatase (AP) enzyme activities of Artemia and rotifer were observed during the enrichment and starvation period (P < 0.05). The digestive enzymes derived from live food to fish larvae provided the highest contribution at the end of the enrichment period. In conclusion, the results of the study provide important contributions to determine the most

  19. Key Performance Characteristics of Organic Shrimp Aquaculture in Southwest Bangladesh

    Directory of Open Access Journals (Sweden)

    Christian Reinhard Vogl

    2012-05-01

    Full Text Available In Bangladesh, black tiger shrimp (Penaeus monodon; Fabricius, 1798 aquaculture has come to be one of the most important sectors in both the rural and national economies. Likewise, organic shrimp aquaculture has emerged as an alternative farming enterprise for farmers especially in the southwestern districts of Bangladesh. The present study aims to show key performance characteristics of organic shrimp farmers and farming in a prototypical shrimp farming area in Bangladesh. Data was collected in 2009 from organic shrimp farmers in the Kaligonj and Shyamnagar sub-districts through questionnaire interviews, transect walks and focus group discussions. The mean productivity of organic shrimp farming in the area is 320 kg ha−1 yr−1 (ranging from 120 to 711 kg ha−1year−1. Organic farmers are more likely to have a higher monthly income and less aquaculture experience. Moreover, suitable landholdings and classified labor distribution have been found to play an important role in the development of organic shrimp aquaculture. The most common assets of organic shrimp aquaculture are high yield, low production cost, available post larvae and high market prices. Small business farmers are likely to earn more income benefits from organic shrimp aquaculture than their larger-scale counterparts. Finally, the paper suggests that more research is needed to stimulate the success of organic shrimp aquaculture.

  20. Brine: a computer program to compute brine migration adjacent to a nuclear waste canister in a salt repository

    International Nuclear Information System (INIS)

    Duckworth, G.D.; Fuller, M.E.

    1980-01-01

    This report presents a mathematical model used to predict brine migration toward a nuclear waste canister in a bedded salt repository. The mathematical model is implemented in a computer program called BRINE. The program is written in FORTRAN and executes in the batch mode on a CDC 7600. A description of the program input requirements and output available is included. Samples of input and output are given

  1. Measurement and modeling of CO2 solubility in NaCl brine and CO2–saturated NaCl brine density

    DEFF Research Database (Denmark)

    Yan, Wei; Huang, Shengli; Stenby, Erling Halfdan

    2011-01-01

    over climate change and energy security. This work is an experimental and modeling study of two fundamental properties in high pressure CO2–NaCl brine equilibrium, i.e., CO2 solubility in NaCl brine and CO2–saturated NaCl brine density. A literature review of the available data was presented first...

  2. Comprehensive Assessment of Marine Coatings in the Laboratory and Field

    Science.gov (United States)

    2015-03-27

    assays. The leachate from coatings was used to conduct assays of survivorship with approximately 100 nauplii larvae of Artemia sp. (brine shrimp ...The larvae were exposed to the coating leachate and their survival is monitored for 2 days. Survival of larvae in coating leachate was compared to... larvae {Balanus amphitrite) were "drop assayed" on each of the replicate surfaces. The assays last approximately 48 h; although exact duration depended

  3. In vivo and in vitro antiplasmodial activities of some plants traditionally used in Guatemala against malaria.

    OpenAIRE

    Franssen, F F; Smeijsters, L J; Berger, I; Medinilla Aldana, B E

    1997-01-01

    We present an evaluation of the antiplasmodial and cytotoxic effects of four plants commonly used in Guatemalan folk medicine against malaria. Methanol extracts of Simarouba glauca D. C., Sansevieria guineensis Willd, Croton guatemalensis Lotsy, and Neurolaena lobata (L.)R.Br. significantly reduced parasitemias in Plasmodium berghei-infected mice. Dichloromethane fractions were screened for their cytotoxicities on Artemia salina (brine shrimp) larvae, and 50% inhibitory concentrations were de...

  4. Toxicity and trophic transfer of P25 TiO2 NPs from Dunaliella salina to Artemia salina: Effect of dietary and waterborne exposure.

    Science.gov (United States)

    Bhuvaneshwari, M; Thiagarajan, Vignesh; Nemade, Prateek; Chandrasekaran, N; Mukherjee, Amitava

    2018-01-01

    The recent increase in nanoparticle (P25 TiO 2 NPs) usage has led to concerns regarding their potential implications on environment and human health. The food chain is the central pathway for nanoparticle transfer from lower to high trophic level organisms. The current study relies on the investigation of toxicity and trophic transfer potential of TiO 2 NPs from marine algae Dunaliella salina to marine crustacean Artemia salina. Toxicity was measured in two different modes of exposure such as waterborne (exposure of TiO 2 NPs to Artemia) and dietary exposure (NP-accumulated algal cells are used to feed the Artemia). The toxicity and accumulation of TiO 2 NPs in marine algae D. salina were also studied. Artemia was found to be more sensitive to TiO 2 NPs (48h LC 50 of 4.21mgL -1 ) as compared to marine algae, D. salina (48h LC 50 of 11.35mgL -1 ). The toxicity, uptake, and accumulation of TiO 2 NPs were observed to be more in waterborne exposure as compared to dietary exposure. Waterborne exposure seemed to cause higher ROS production and antioxidant enzyme (SOD and CAT) activity as compared to dietary exposure of TiO 2 NPs in Artemia. There were no observed biomagnification (BMF) and trophic transfer from algae to Artemia through dietary exposure. Histopathological studies confirmed the morphological and internal damages in Artemia. This study reiterates the possible effects of the different modes of exposure on trophic transfer potential of TiO 2 NPs and eventually the consequences on aquatic environment. Copyright © 2017 Elsevier Inc. All rights reserved.

  5. A Bio-economic Model of a Shrimp Hatchery in the Mekong River Delta of Vietnam

    OpenAIRE

    Sinh, L.X.; MacAulay, T. Gordon; Brennan, Donna C.

    2003-01-01

    Shrimp culture areas and production of the Mekong Delta cover about 60 percent of the total shrimp areas and production of Vietnam. Especially, the Delta contributes about 80 percent of the total shrimp production for export. Rapid development of the shrimp industry is raising a number of serious problems that need to be solved. Shrimp seed supply (post larvae production) plays an essential role in the shrimp industry and it is one of the most important constraints to the development of the s...

  6. Thermal-gradient migration of brine inclusions in salt crystals

    International Nuclear Information System (INIS)

    Yagnik, S.K.

    1982-09-01

    It has been proposed that high-level nuclear waste be disposed in a geologic repository. Natural-salt deposits, which are being considered for this purpose, contain a small volume fraction of water in the form of brine inclusions distributed throughout the salt. Radioactive-decay heating of the nuclear wastes will impose a temperature gradient on the surrounding salt which mobilizes the brine inclusions. Inclusions filled completely with brine migrate up the temperature gradient and eventually accumulate brine near the buried waste forms. The brine may slowly corrode or degrade the waste forms which is undesirable. In this work, thermal gradient migration of both all-liquid and gas-liquid inclusions was experimentally studied in synthetic single crystals of NaCl and KCl using a hot-stage attachment to an optical microscope which was capable of imposing temperature gradients and axial compressive loads on the crystals. The migration velocities of the inclusions were found to be dependent on temperature, temperature gradient, and inclusion shape and size. The velocities were also dictated by the interfacial mass transfer resistance at brine/solid interface. This interfacial resistance depends on the dislocation density in the crystal, which in turn, depends on the axial compressive loading of the crystal. At low axial loads, the dependence between the velocity and temperature gradient is non-linear.At high axial loads, however, the interfacial resistance is reduced and the migration velocity depends linearly on the temperature gradient. All-liquid inclusions filled with mixed brines were also studied. For gas-liquid inclusions, three different gas phases (helium, air and argon) were compared. Migration studies were also conducted on single crystallites of natural salt as well as in polycrystalline natural salt samples. The behavior of the inclusions at large angle grain boundaries was observed. 35 figures, 3 tables

  7. Effects of brine migration on waste storage systems. Final report

    International Nuclear Information System (INIS)

    Gaffney, E.S.; Nickell, R.E.

    1979-01-01

    Processes which can lead to mobilization of brine adjacent to spent fuel or nuclear waste canisters and some of the thermomechanical consequences have been investigated. Velocities as high as 4 x 10 -7 m s -1 (13 m y -1 ) are calculated at the salt/canister boundary. As much as 40 liters of pure NaCl brine could accumulate around each canister during a 10-year storage period. Accumulations of bittern brines would probably be less, in the range of 2 to 5 liters. With 0.5% water, NaCl brine accumulation over a 10-year storage cycle around a spent fuel canister producing 0.6 kW of heat is expected to be less than 1 liter for centimeter-size inclusions and less than 0.5 liter for millimeter-size inclusions. For bittern brines, about 25 years would be required to accumulate 0.4 liter. The most serious mechanical consequence of brine migration would be the increased mobility of the waste canister due to pressure solution. In pressure solution enhanced deformation, the existence of a thin film of fluid either between grains or between media (such as between a canister and the salt) provides a pathway by which the salt can be redistributed leading to a marked increase in strain rates in wet rock relative to dry rock. In salt, intergranular water will probably form discontinuous layers rather than films so that they would dominate pressure solution. A mathematical model of pressure solution indicates that pressure solution will not lead to appreciable canister motions except possibly in fine grained rocks (less than 10 -4 m). In fine grained salts, details of the contact surface between the canister and the salt bed may lead to large pressure solution motions. A numerical model indicates that heat transfer in the brine layer surrounding a spent fuel canister is not conduction dominated but has a significant convective component

  8. Thermal gradient migration of brine inclusions in salt crystals

    International Nuclear Information System (INIS)

    Yagnik, S.K.

    1982-01-01

    Natural salt deposits, which are being considered for high-level nuclear wastes repositories, contain a small volume fraction of water in the form of brine inclusions distributed throughout the salt. Radioactive decay heating of the nuclear wastes will impose a temperature gradient on the surrounding salt which mobilizes the brine inclusions. Inclusions filled completely with brine (the all-liquid inclusions) migrate up the temperature gradient and eventually accumulate brine near the buried waste forms. The brine may slowly corrode or degrade the waste forms which is undesirable. In the present work, thermal gradient migration of both all-liquid and gas-liquid inclusions was experimentally studied in synthetic single crystals of NaCl and KCl using a hot-stage attachment to an optical microscope which was capable of imposing temperature gradients and axial compressive loads on the crystals. The migration velocities of the inclusions were found to be dependent on temperature, temperature gradient, and inclusion shape and size. The velocities were also dictated by the interfacial mass transfer resistance at brine/solid interface. This interfacial resistance depends on the dislocation density in the crystal, which in turn, depends on the axial compressive loading of the crystal. At low axial loads, the dependence between the velocity and temperature gradient is nonlinear. At high axial loads, however, the interfacial resistance is reduced and the migration velocity depends linearly on the temperature gradient. All-liquid inclusions filled with mixed brines were also studied. For gas-liquid inclusions, three different gas phases (helium, air and argon) were compared. Migration studies were also conducted on single crystallites of natural salt as well as in polycrystalline natural salt samples. The behavior of the inclusions at large angle grain boudaries was observed

  9. The impact of shrimp farming on mangrove ecosystems

    DEFF Research Database (Denmark)

    Ashton, Elizabeth Clare

    2008-01-01

    . Policy to position shrimp farms behind mangroves can be effective but also requires good institutional capacity and coordination, effective enforcement, incentives, land tenure and participation of all stakeholders for success. Better management practices have been identified which reduce impacts......Farmed shrimp production and value continue to increase with Asia producing the global majority of shrimp and the USA, Japan and Europe being the main importers. Shrimp farming systems are very diverse in their management, size and impacts. There are many causes for mangrove loss but the conversion...... of mangroves to shrimp farms has caused considerable attention. The major issues of shrimp farming include the loss of important ecological and socio-economic functions of mangrove ecosystems, changes in hydrology, salinization, introduction of non-native species and diseases, pollution from effluents...

  10. Shrimp aquaculture in low salinity water feeded with worm flavor

    Directory of Open Access Journals (Sweden)

    Wenceslao Valenzuela Quiñónez

    2012-09-01

    Full Text Available Shrimp aquaculture in Sinaloa is one of the top economic enterprises, generating many jobs and earns significant incomes every year. Shrimp feed is an essential part of maintaining healthy production. In this initial approach of shrimp growth in low salinity water, were tested two formulas of animal protein composed of 40% (APL1 and 20% (APL2 worm protein, a commercial diet, and no supplementary feed. Physicochemical parameters did not have a direct influence in shrimpbehavior. After six weeks of experimentation, shrimp fed with commercial diet had a weight gain 20% higher than those feed with worm protein. There were no significantly differences between sizes with respect to 40% animal protein and 20% animal protein with the commercial diet (P  0.05. However, shrimp fed worm protein had lower mortality. The use of worm protein could be an option to maintain a high quantity of shrimp reared in low salinity waters.

  11. Comparison of bioassays using the anostracan crustaceans Artemia salina and Thamnocephalus platyurus for plant extract toxicity screening Comparação de bioensaios com os crustáceos Artemia salina e Thamnocephalus platyurus para abordagem de extratos de plantas com toxicidade

    OpenAIRE

    Pablo Mayorga; Karen R. Pérez; Sully M. Cruz; Armando Cáceres

    2010-01-01

    Three lethality bioassays, using the salt-water crustacean Artemia salina Leach, Artemiidae, (conventional 96 microwell plate test and the Artoxkit M microbiotest) and the freshwater crustacean Thamnocephalus platyurus Packard, Thamnocephalidae, (Thamnotoxkit F microbiotest), were compared using extracts of ten Guatemalan plant species. It was previously observed that five of them have anti-Artemia activity. These were: Solanum americanum Mill., Solanaceae, Gliricidia sepium (Jacq.) Kunth ex ...

  12. Market integration of cold and warmwater shrimp in Europe

    DEFF Research Database (Denmark)

    Ankamah-Yeboah, Isaac; Ståhl, Lisa; Nielsen, Max

    2017-01-01

    ) holds in three of the five cases. For processed shrimp, the LOP holds in three of four integrated cases, and for retail sales in the UK and Denmark, the LOP fails to hold in the presence of market integration. Unprocessed coldwater shrimp leads the market in northern Europe. Downstream, prices adjust......This study examines market integration between the cold and warmwater shrimp value chain in the UK, Denmark, Italy, Sweden, and Norway using cointegration methods. For all countries, market integration exists between cold and warmwater unprocessed shrimp imports, where the law of one price (LOP...

  13. Shrimp viral diseases, import risk assessment and international trade.

    Science.gov (United States)

    Karunasagar, Iddya; Ababouch, Lahsen

    2012-09-01

    Shrimp is an important commodity in international trade accounting for 15 % in terms of value of internationally traded seafood products which reached $102.00 billion in 2008. Aquaculture contributes to over 50 % of global shrimp production. One of the major constraints faced by shrimp aquaculture is the loss due to viral diseases like white spot syndrome, yellow head disease, and Taura syndrome. There are several examples of global spread of shrimp diseases due to importation of live shrimp for aquaculture. Though millions of tonnes of frozen or processed shrimp have been traded internationally during the last two decades despite prevalence of viral diseases in shrimp producing areas in Asia and the Americas, there is no evidence of diseases having been transmitted through shrimp imported for human consumption. The guidelines developed by the World Animal Health Organisation for movement of live animals for aquaculture, frozen crustaceans for human consumption, and the regulations implemented by some shrimp importing regions in the world are reviewed.

  14. A review of the chemical aspects of irradiated shrimp

    International Nuclear Information System (INIS)

    Shamsuzzaman, K.

    1989-09-01

    The literature was reviewed for information on the chemical aspects of irradiated shrimp. Low-dose irradiation can effectively control spoilage and pathogenic organisms and extend the refrigerated shelf life of shrimp. Radiation-induced increases in black discoloration occur in some species during storage, but can be minimized by proper blanching. Some loss of the characteristic pink color occurs at doses above 2.5 kGy, and irradiation off-odors occur above 1.5 kGy. These changes can be minimized by irradiating the shrimp when they are frozen. No significant change in protein, fat, carbohydrate, and ash content occurs as a result of low-dose irradiation. Low-dose irradiation does not produce any detectable change in the levels of volatile carbonyl compounds; irradiation at 8 kGy results in a transient increase in the total volatile compounds during subsequent storage in ice, but the increase is higher in the unirradiated shrimp. Radiation-induced changes in the fatty acid composition of shrimp are small. Some minor changes in the amino acid composition occur in irradiated shrimp; similar changes occur due to other processes such as canning and drying in hot air. Some vitamins in shrimp such as thiamine are affected by irradiation. But the loss is less extensive than in thermally processed shrimp. The protein efficiency ratio is not affected by irradiation of shrimp, and no adverse effects attributed to irradiation were found in animal feeding studies

  15. Chemistry of glass corrosion in high saline brines

    International Nuclear Information System (INIS)

    Grambow, B.; Mueller, R.

    1990-01-01

    Corrosion data obtained in laboratory tests can be used for the performance assessment of nuclear waste glasses in a repository if the data are quantitatively described in the frame of a geochemical model. Experimental data were obtained for conventional pH values corrected for liquid junction, amorphous silica solubility and glass corrosion in concentrated salt brines. The data were interpreted with a geochemical model. The brine chemistry was described with the Pitzer formalism using a data base which allows calculation of brine compositions in equilibrium with salt minerals at temperatures up to 200C. In MgCl 2 dominated brines Mg silicates form and due to the consumption of Mg the pH decreases with proceeding reaction. A constant pH (about 4) and composition of alteration products is achieved, when the alkali release from the glass balances the Mg consumption. The low pH results in high release of rare earth elements REE (rare earth elements) and U from the glass. In the NaCl dominated brine MgCl 2 becomes exhausted by Mg silicate formation. As long as there is still Mg left in solution the pH decreases. After exhaustion of Mg the pH rises with the alkali release from the glass and analcime is formed

  16. Searching for brine on Mars using Raman spectroscopy

    Energy Technology Data Exchange (ETDEWEB)

    Fischer, E.

    2016-07-01

    In the last few years, water ice and perchlorate salts capable of melting this ice and producing liquid solutions have been discovered at the surface and shallow subsurface of Mars. In addition to via melting of ice, perchlorate salts may also form liquid solutions by absorbing water vapor when the relative humidity is above a certain threshold in a process known as deliquescence. Formed either by melting or deliquescence, liquid solutions (brine) are the most likely way of liquid water activity on the Martian surface and in the shallow subsurface and are therefore important to understand the habitability of Mars. Using Raman spectroscopy, we provide reference spectra of various mixing states of liquid water, water ice and calcium perchlorate, all of which can occur during brine formation. We focus on the perchlorate symmetric stretching band and the O-H stretching vibrational band to distinguish brine from crystalline salt and water ice. We show that perchlorate brines can be identified by analyzing the peaks and their widths in the decomposed Raman spectra of the investigated samples. This serves as an important reference for future in-situ Raman spectrometers on Mars, such as those on the ExoMars and Mars 2020 rovers and can aid in the detection of brine formation on Mars. (Author)

  17. Stability of polyvinyl alcohol-coated biochar nanoparticles in brine

    Energy Technology Data Exchange (ETDEWEB)

    Griffith, Christopher, E-mail: cgriffith@utexas.edu; Daigle, Hugh [University of Texas at Austin, Department of Petroleum and Geosystems Engineering (United States)

    2017-01-15

    This paper reports on the dispersion stability of 150 nm polyvinyl alcohol coated biochar nanoparticles in brine water. Biochar is a renewable, carbon based material that is of significant interest for enhanced oil recovery operations primarily due to its wide ranging surface properties, low cost of synthesis, and low environmental toxicity. Nanoparticles used as stabilizing agents for foams (and emulsions) or in nanofluids have emerged as potential alternatives to surfactants for subsurface applications due to their improved stability at reservoir conditions. If, however, the particles are not properly designed, they are susceptible to aggregation because of the high salinity brines typical of oil and gas reservoirs. Attachment of polymers to the nanoparticle surface, through covalent bonds, provides steric stabilization, and is a necessary step. Our results show that as the graft density of polyvinyl alcohol increases, so too does the stability of nanoparticles in brine solutions. A maximum of 34 wt% of 50,000 Da polyvinyl alcohol was grafted to the particle surface, and the size of the particles was reduced from ~3500 nm (no coating) to 350 nm in brine. After 24 h, the particles had a size of ~500 nm, and after 48 h completely aggregated. 100,000 Da PVA coated at 24 wt% on the biochar particles were stable in brine for over 1 month with no change in mean particle size of ~330 nm.

  18. Evaluation of the photodynamic activity of Xanthene Dyes on Artemia salina described by chemometric approaches

    Directory of Open Access Journals (Sweden)

    Diogo S. Pellosi

    2013-10-01

    Full Text Available The development of drugs for photodynamic therapy (PDT is an important area of research due to their growing use in medical applications. Therefore, it is important to develop new bioassay methods for PDT photosensitizers that are inexpensive, easy to handle and highly sensitive to environmental conditions. Xanthene dyes (fluorescein, rose bengal B, erythrosine B and eosin Y with LED light sources were investigated using Artemia salina as a bioindicator of photodynamic activity. In this study, three factors were investigated: (i photosensitizers concentration, (ii the LED irradiation time and (iii the waiting time between the addition of the photosensitizers and the beginning of the irradiation. To analyze the photo-killing of A. salina, it was employed a 23 full factorial design. The death of A. salina was related to dye structure and the interaction between the irradiation time and the photosensitizers concentration. About 60% of crustaceans death was obtained using rose bengal B, which presentes the highest quantum yield of singlet oxygen due to the number of iodide substituents in the xanthenes ring. The proposed bioassay using A. salina, xanthene dyes and LED irradiation was found suitable for quantitative PDT drug evaluation.

  19. Study of interaction of low doses of ionizing radiation with selected pesticides on Artemia franciscana

    Directory of Open Access Journals (Sweden)

    Michaela Špalková

    2015-01-01

    Full Text Available The aim of our study was to observe the effect of low doses of ionizing radiation, azoxystrobin and glyphosate separately as well as in their mutual combinations on the lethality of Artemia franciscana. Increasing intensity of agricultural production leads to continual exposure of animals to pesticide residues. Animals could be exposed to these negative factors in case of possible nuclear accident. A total of 1000 nauplia, randomly placed in 20 groups (1 control and 19 experimental groups, were irradiated with gamma rays (0, 20, 30, 40 or 50 Gy and were kept in solution of standardized sea water and standardized sea water with addition of pesticides (azoxystrobin at a concentration of 0.0001 g·l-1 and glyphosate at a concentration of 0.5 g·l-1 individually or in combination. A significant increase of lethality was observed after 48, 72 and 96 h of exposure to only azoxystrobin, but also in the group exposed to the combined effect of azoxystrobin and glyphosate at all time intervals. On the other hand, a significant decrease of lethality was observed in those groups exposed to ionizing radiation even at the dose of 30 Gy at all time intervals compared to non-irradiated groups. These results confirm the toxicity of azoxystrobin to aquatic organisms; non-toxicity of glyphosate to crustaceans and support the theory of radiation hormesis at the level of 30 Gy radiation dose.

  20. Isolasi Senyawa Aktif Ekstrak Etanol Biji Alpukat (Persea americana dan Uji Toksisitas Terhadap Artemia Salina Leach

    Directory of Open Access Journals (Sweden)

    Andi Nur Fitriani Abubakar

    2014-07-01

    Full Text Available Avocado seed (Persea americana is recognized as one of medicinal plants. It contains several secondary metabolites, which have toxic activity. However, efforts to identify active compounds from avocado seeds (Persea americana are still relatively rare. Therefore, isolation and toxicity assay have been conducted foward the active compound of avocado seed. Maceration one kilogram of seed dried powder by ethanol obtained 49,7464 gram extract. Separation of etanol extract by column chromatography generated 0,0698 grams of pure white needle crytal, which is positively triterpenoid based on Lieberman-Buchard test. In addition, infrared spectrum showed the existence of OH, C=C, C-C, C=O, -C-H, -CH3,-CH2 and C-O stretch, which support the presumed compound. The result of toxicity test on Artemia salina Leach showed that the extract, fraction and pure isolates of the etanol extract are toxic with LC50 values 13,274 g/mL; 9,528 g/mL and 8,128 g/mL, respectively.

  1. Extreme morphologies of mantis shrimp larvae

    OpenAIRE

    Haug, Carolin; Ahyong, Shane T.; Wiethase, Joris H.; Olesen, Jørgen; Haug, Joachim T.

    2016-01-01

    ABSTRACT Larvae of stomatopods (mantis shrimps) are generally categorized into four larval types: antizoea, pseudozoea (both representing early larval stages), alima and erichthus (the latter two representing later larval stages). These categories, however, do not reflect the existing morphological diversity of stomatopod larvae, which is largely unstudied. We describe here four previously unknown larval types with extreme morphologies. All specimens were found in the collections of the Zoolo...

  2. Vitamin requirements of juvenile penaeid shrimp

    OpenAIRE

    Conklin, D

    1989-01-01

    The results of supplementing crustacean feeds with vitamins are examined specifically from the standpoint of shrimp culture. Micro-nutrients selected for discussion include: water-soluble vitamins of the B-complex, choline and inositol, vitamin C and the fat-soluble group of vitamins: A, D, E and K. Ways in which utilization of vitamins and ultimately dietary demand are altered by physiological state, conditions of culture, as well as factors which impact on feed levels, are explored.

  3. Vortex formation with a snapping shrimp claw.

    Science.gov (United States)

    Hess, David; Brücker, Christoph; Hegner, Franziska; Balmert, Alexander; Bleckmann, Horst

    2013-01-01

    Snapping shrimp use one oversized claw to generate a cavitating high speed water jet for hunting, defence and communication. This work is an experimental investigation about the jet generation. Snapping shrimp (Alpheus-bellulus) were investigated by using an enlarged transparent model reproducing the closure of the snapper claw. Flow inside the model was studied using both High-Speed Particle Image Velocimetry (HS-PIV) and flow visualization. During claw closure a channel-like cavity was formed between the plunger and the socket featuring a nozzle-type contour at the orifice. Closing the mechanism led to the formation of a leading vortex ring with a dimensionless formation number of approximate ΔT*≈4. This indicates that the claw might work at maximum efficiency, i.e. maximum vortex strength was achieved by a minimum of fluid volume ejected. The subsequent vortex cavitation with the formation of an axial reentrant jet is a reasonable explanation for the large penetration depth of the water jet. That snapping shrimp can reach with their claw-induced flow. Within such a cavitation process, an axial reentrant jet is generated in the hollow cylindrical core of the cavitated vortex that pushes the front further downstream and whose length can exceed the initial jet penetration depth by several times.

  4. Vortex formation with a snapping shrimp claw.

    Directory of Open Access Journals (Sweden)

    David Hess

    Full Text Available Snapping shrimp use one oversized claw to generate a cavitating high speed water jet for hunting, defence and communication. This work is an experimental investigation about the jet generation. Snapping shrimp (Alpheus-bellulus were investigated by using an enlarged transparent model reproducing the closure of the snapper claw. Flow inside the model was studied using both High-Speed Particle Image Velocimetry (HS-PIV and flow visualization. During claw closure a channel-like cavity was formed between the plunger and the socket featuring a nozzle-type contour at the orifice. Closing the mechanism led to the formation of a leading vortex ring with a dimensionless formation number of approximate ΔT*≈4. This indicates that the claw might work at maximum efficiency, i.e. maximum vortex strength was achieved by a minimum of fluid volume ejected. The subsequent vortex cavitation with the formation of an axial reentrant jet is a reasonable explanation for the large penetration depth of the water jet. That snapping shrimp can reach with their claw-induced flow. Within such a cavitation process, an axial reentrant jet is generated in the hollow cylindrical core of the cavitated vortex that pushes the front further downstream and whose length can exceed the initial jet penetration depth by several times.

  5. Brine Migration in Heated Salt: Lessons Learned from Field Experiments

    Science.gov (United States)

    Kuhlman, K. L.; Matteo, E. N.; Mills, M.

    2017-12-01

    We summarize several interesting brine migration related phenomena hinted at in field experiments from field testing related to salt radioactive waste repositories in Germany and the US. Past heater tests in salt have shown 1) thermal-hydrological-mechanical coupling is quite strong during both heating and cooling; 2) chemical composition of brine evolves during heating, and comprises a mix of several water sources; and 3) acid gas (HCl) generation has been observed during past heater tests and may have multiple mechanisms for formation. We present a heated brine migration test design, formulated with these complexities in mind. Sandia National Laboratories is a multimission laboratory managed and operated by National Technology and Engineering Solutions of Sandia LLC, a wholly owned subsidiary of Honeywell International Inc. for the U.S. Department of Energy's National Nuclear Security Administration under contract DE-NA0003525.

  6. Microbially mediated barite dissolution in anoxic brines

    International Nuclear Information System (INIS)

    Ouyang, Bingjie; Akob, Denise M.; Dunlap, Darren; Renock, Devon

    2017-01-01

    Fluids injected into shale formations during hydraulic fracturing of black shale return with extraordinarily high total-dissolved-solids (TDS) and high concentrations of barium (Ba) and radium (Ra). Barite, BaSO_4, has been implicated as a possible source of Ba as well as a problematic mineral scale that forms on internal well surfaces, often in close association with radiobarite, (Ba,Ra)SO_4. The dissolution of barite by abiotic processes is well quantified. However, the identification of microbial communities in flowback and produced water necessitates the need to understand barite dissolution in the presence of bacteria. Therefore, we evaluated the rates and mechanisms of abiotic and microbially-mediated barite dissolution under anoxic and hypersaline conditions in the laboratory. Barite dissolution experiments were conducted with bacterial enrichment cultures established from produced water from Marcellus Shale wells located in northcentral Pennsylvania. These cultures were dominated by anaerobic halophilic bacteria from the genus Halanaerobium. Dissolved Ba was determined by ICP-OES and barite surfaces were investigated by SEM and AFM. Our results reveal that: 1) higher amounts of barium (up to ∼5 × ) are released from barite in the presence of Halanaerobium cultures compared to brine controls after 30 days of reaction, 2) etch pits that develop on the barite (001) surface in the presence of Halanaerobium exhibit a morphology that is distinct from those that form during control experiments without bacteria, 3) etch pits that develop in the presence of Halanaerobium exhibit a morphology that is similar to the morphology of etch pits formed in the presence of strong organic chelators, EDTA and DTPA, and 4) experiments using dialysis membranes to separate barite from bacteria suggest that direct contact between the two is not required in order to promote dissolution. These results suggest that Halanaerobium increase the rate of barite dissolution in anoxic

  7. Guidelines to Facilitate the Evaluation of Brines for Winter Roadway Maintenance Operations.

    Science.gov (United States)

    2017-09-19

    This document presents guidelines to facilitate the evaluation of brines for winter weather roadway maintenance applications in Texas. Brines are used in anti-icing applications which typically consist of placing liquid snow and ice control chemicals...

  8. A deep sea community at the Kebrit brine pool in the Red Sea

    KAUST Repository

    Vestheim, Hege; Kaartvedt, Stein

    2015-01-01

    Approximately 25 deep sea brine pools occur along the mid axis of the Red Sea. These hypersaline, anoxic, and acidic environments have previously been reported to host diverse microbial communities. We visited the Kebrit brine pool in April 2013

  9. Effect of low doses of ionizing radiation, azoxystrobin and selected heavy metals on the model organism of species Artemia franciscana; Posobenie nizkych davok ionizujuceho ziarenia, azoxystrobinu a vybranych tazkych kovov na modelovy organizmus druhu Artemia franciscana

    Energy Technology Data Exchange (ETDEWEB)

    Spalkova, M.; Danova, D. [Univerzita veterinarskeho lekarstva a farmacie, Katedra biologie a genetiky, Ustav biologie, zoologie a radiobiologie, 04181 Kosice (Slovakia); Falis, M. [Univerzita veterinarskeho lekarstva a farmacie, Katedra farmakologie a toxikologie, Ustav toxikologie, 04181 Kosice (Slovakia); Zdarsky, M. [Veterinarni a farmaceuticka univerzita Brno, Ustav biochemie, chemie a biofyziky, 61242 Brno (Czech Republic)

    2013-04-16

    The aim of our study was to observe the effect of low doses of ionizing radiation, azoxystrobin, zinc and chrome on the lethality of Artemia franciscana. Significant increase of the lethality was observed after 72 and 96 hours of exposure to azoxystrobin and chrome as well as to azoxystrobin, chrome and irradiation. On the other hand, we observed significant decrease of the lethality in the groups exposed to pesticide and chrome or pesticide and zinc in combination with ionizing radiation with the dose of 50 Gy at time interval of 96 hours in comparison to non-irradiated groups. (authors)

  10. Prospecção fitoquímica de Sonchus oleraceus e sua toxicidade sobre o microcrustáceo Artemia salina Phytochemical prospecting of Sonchus oleraceus and its toxicity to Artemia salina

    OpenAIRE

    J.M. Lima; C.A. Silva; M.B. Rosa; J.B. Santos; T.G. Oliveira; M.B. Silva

    2009-01-01

    A espécie vegetal Sonchus oleraceus é uma planta daninha presente em diversas culturas no Brasil e de utilização na medicina popular. Neste trabalho, realizou-se a prospecção fitoquímica dessa espécie com extratos em etanol, água e diclorometano, bem como testes de toxicidade sobre o microcrustáceo Artemia salina. O extrato aquoso apresentou em sua composição açúcares redutores, compostos fenólicos, taninos, flavonóides e cumarinas. No extrato etanólico, observaram-se os mesmos compostos qual...

  11. Composición del contenido de ácidos grasos en tres poblaciones mexicanas de Artemia franciscana de aguas epicontinentales

    Directory of Open Access Journals (Sweden)

    Aída Malpica Sánchez

    2004-03-01

    Full Text Available En este trabajo se presenta la composición y el porcentaje de ácidos grasos de tres poblaciones mexicanas de Artemia franciscana de aguas epicontinentales; dos provienen de lugares silvestres (Coahuila y San Luis Potosí y una (Texcoco de un cultivo alimentado con Spirulina. Para la determinación de los ácidos grasos de cada población se extrajeron los lípidos totales por el método soxhlet y se esterificaron los ácidos grasos; posteriormente, mediante cromatografía de gases se determinó la composición y el porcentaje de los mismos. Los resultados muestran que la población de Texcoco contiene los seis ácidos grasos relevantes para peces y crustáceos en la acuicultura (16:0; 16:1; 18:1; 18:2w6; 18:3w3 y 20:5w3; mientras que la Artemia de San Luis Potosí resultó ser la más pobre en estos ácidos y la población de Coahuila, aunque mostró un perfil amplio, carece del ácido linolénico, ácido esencial y precursor de otros. Al comparar estos resultados, con datos ecológicos de estas tres poblaciones publicados previamente, se puede señalar que el ambiente es determinante para este crustáceo ya que Artemia de Texcoco provenía de un cultivo alimentado con Spirulina y fue la población que mostró mayor variedad de ácidos grasos. Las otras dos poblaciones son silvestres, con hábitats diferentes, la Artemia de Coahuila vive en aguas ricas en sulfatos y la de San Luis Potosí habita en vasos evaporadores de sal construidos con piedra de cantera y por lo tanto con escaso crecimiento de fitoplancton, ambas poblaciones mostraron deficiencias de ácidos grasos esenciales, sobre todo ésta últimaIn this paper is presented the percentage of fatty acids composition of three Artemia franciscana Mexican populations of epicontinentals waters; two are from natural environments (Coahuila and San Luis Potosí and one (Texcoco is a culture fed with Spirulina. Determination of fatty acids composition in each population, was performed by

  12. Bacterial dynamics in intestines of the black tiger shrimp and the Pacific white shrimp during Vibrio harveyi exposure.

    Science.gov (United States)

    Rungrassamee, Wanilada; Klanchui, Amornpan; Maibunkaew, Sawarot; Karoonuthaisiri, Nitsara

    2016-01-01

    The intestinal microbiota play important roles in health of their host, contributing to maintaining the balance and resilience against pathogen. To investigate effects of pathogen to intestinal microbiota, the bacterial dynamics upon a shrimp pathogen, Vibrio harveyi, exposures were determined in two economically important shrimp species; the black tiger shrimp (BT) and the Pacific white shrimp (PW). Both shrimp species were reared under the same diet and environmental conditions. Shrimp survival rates after the V. harveyi exposure revealed that the PW shrimp had a higher resistance to the pathogen than the BT shrimp. The intestinal bacterial profiles were determined by denaturing gradient gel electrophoresis (DGGE) and barcoded pyrosequencing of the 16S rRNA sequences under no pathogen challenge control and under pathogenic V. harveyi challenge. The DGGE profiles showed that the presence of V. harveyi altered the intestinal bacterial patterns in comparison to the control in BT and PW intestines. This implies that bacterial balance in shrimp intestines was disrupted in the presence of V. harveyi. The barcoded pyrosequencing analysis showed the similar bacterial community structures in intestines of BT and PW shrimp under a normal condition. However, during the time course exposure to V. harveyi, the relative abundance of bacteria belong to Vibrio genus was higher in the BT intestines at 12h after the exposure, whereas relative abundance of vibrios was more stable in PW intestines. The principle coordinates analysis based on weighted-UniFrac analysis showed that intestinal bacterial population in the BT shrimp lost their ability to restore their bacterial balance during the 72-h period of exposure to the pathogen, while the PW shrimp were able to reestablish their bacterial population to resemble those seen in the unexposed control group. This observation of bacterial disruption might correlate to different mortality rates observed between the two shrimp species

  13. adoption of improved aquaculture practices by shrimp farmers

    African Journals Online (AJOL)

    Dr.Adesope

    use of check trays and adjustment of feed accordingly (95.0%), formation of ... Key words: adoption, improved aquaculture, shrimp farmers ... Brackish water shrimp farming is getting more attention because of high profitability ..... water and pond water whereas 52.5% of farmers did not observe the actual acclimatisation.

  14. Shrimp Tropomyosin Retains Antibody Reactivity after Exposure to Acidic Conditions

    Science.gov (United States)

    Although shrimp can be found in certain high acid food matrices, the allergenic capacity of shrimp tropomyosin exposed to low pH condition has not been fully clarified. Thus, a model marinade comprising white vinegar adjusted to different pH was used to determine the effects of acid-induced denatura...

  15. Biology, genome organization and evolution of parvoviruses in marine shrimp

    Science.gov (United States)

    A number of parvoviruses are now know to infect marine shrimp, and these viruses alone or in combination with other viruses have the potential to cause major losses in shrimp aquaculture globally. This review provides a comprehensive overview of the biology, genome organization, gene expression, and...

  16. Controlled reproduction of penaeid shrimp: a contribution to its improvement

    NARCIS (Netherlands)

    Alfaro Montoya, J.

    2001-01-01

    This dissertation deals with controlled reproduction of penaeid shrimp. New knowledge about natural reproductive activity of Penaeus occidentalis in Gulf of Nicoya, Costa Rica, is presented. Since in vitro fertilization of open thelycum shrimp proved unsuccessful, a hypothesis is given to

  17. Job Satisfaction in the Shrimp Trawl Fisheries of Vietnam

    Science.gov (United States)

    Sinh, Le Xuan

    2012-01-01

    This paper investigates the job satisfaction of small-scale shrimp trawl fishers in the vicinity of Camau National Park in southern Vietnam. The research sample consisted of 77 fishers who belong to a growing population of shrimp fishers in the region. The results suggest that 60% would change their fishing metier, 78% would leave fishing for…

  18. Northern Shrimp (Pandalus borealis) Recruitment in West Greenland Waters

    DEFF Research Database (Denmark)

    Pedersen, S. A.; Storm, L. M.

    2002-01-01

    Lipid class and fatty acid compositions were determined in shrimp larvae (Pandalus borealis and P. montagui) collected along transects across banks on the West Greenland shelf in June 1999, May and July 2000. The lipid class contents were investigated as indices of larval shrimp lipid condition a...

  19. Defensive enrolment in mantis shrimp larvae (Malacostraca: Stomatopoda)

    NARCIS (Netherlands)

    Haug, C.; Haug, J.T.

    2014-01-01

    We describe a possible new defensive behaviour of larval stages of mantis shrimps (Stomatopoda). Mantis shrimp larvae are rarely observed in nature, thus the study is based on postures of museum material and functional morphological aspects. Specimens described here are tightly enrolled, their pleon

  20. Pink shrimp as an indicator for restoration of everglades ecosystems

    Science.gov (United States)

    Browder, Joan A.; Robblee, M.B.

    2009-01-01

    The pink shrimp, Farfantepenaeus duorarum, familiar to most Floridians as either food or bait shrimp, is ubiquitous in South Florida coastal and offshore waters and is proposed as an indicator for assessing restoration of South Florida's southern estuaries: Florida Bay, Biscayne Bay, and the mangrove estuaries of the lower southwest coast. Relationships between pink shrimp and salinity have been determined in both field and laboratory studies. Salinity is directly relevant to restoration because the salinity regimes of South Florida estuaries, critical nursery habitat for the pink shrimp, will be altered by changes in the quantity, timing, and distribution of freshwater inflow planned as part of the Comprehensive Everglades Restoration Project (CERP). Here we suggest performance measures based on pink shrimp density (number per square meter) in the estuaries and propose a restoration assessment and scoring scheme using these performance measures that can readily be communicated to managers, policy makers, and the interested public. The pink shrimp is an appropriate restoration indicator because of its ecological as well as its economic importance and also because scientific interest in pink shrimp in South Florida has produced a wealth of information about the species and relatively long time series of data on both juveniles in estuarine nursery habitats and adults on the fishing grounds. We suggest research needs for improving the pink shrimp performance measure.

  1. Population Structure and Recruitment of Penaeid Shrimps from the ...

    African Journals Online (AJOL)

    larger shrimps located in deeper waters (Brito et al.,. 1998; Abdula ... species, the role of the shallow coastal areas (less than 8-10 m) of the ... and define the recruitment season of penaeid shrimps ... later processing in the laboratory. A 0.5 L ...

  2. Job Satisfaction in the Shrimp Trawl Fisheries of Chennai, India

    Science.gov (United States)

    Bavinck, Maarten

    2012-01-01

    Shrimp trawling represents an important fishing metier in South India, generating high levels of employment and economic value. It is also a contested metier, ostensibly contributing to environmental degradation and social inequality. This paper investigates the job satisfaction of crew members (captains and workers) on board the shrimp trawlers…

  3. Carriage of vibrio species by shrimps harvested from the coastal ...

    African Journals Online (AJOL)

    Objectives: To determine the prevalence of Vibrio spp in unprocessed shrimps and their susceptibility to antibiotics. Design: A prospective study of Vibrio spp associated with shrimps harvested from the coastal waters of South West Cameroon. Setting: A laboratory based study at the Department of Life Sciences, University ...

  4. Effect of organic acids on shrimp pathogen, Vibrio harveyi.

    Science.gov (United States)

    Mine, Saori; Boopathy, Raj

    2011-07-01

    Shrimp farming accounts for more than 40% of the world shrimp production. Luminous vibriosis is a shrimp disease that causes major economic losses in the shrimp industry as a result of massive shrimp kills due to infection. Some farms in the South Asia use antibiotics to control Vibrio harveyi, a responsible pathogen for luminous vibriosis. However, the antibiotic-resistant strain was found recently in many shrimp farms, which makes it necessary to develop alternative pathogen control methods. Short-chain fatty acids are metabolic products of organisms, and they have been used as food preservatives for a long time. Organic acids are also commonly added in feeds in animal husbandry, but not in aquaculture. In this study, growth inhibitory effects of short-chain fatty acids, namely formic acid, acetic acid, propionic acid, and butyric acid, on V. harveyi were investigated. Among four acids, formic acid showed the strongest inhibitory effect followed by acetic acid, propionic acid, and butyric acid. The minimum inhibitory concentration (MIC) of 0.035% formic acid suppressed growth of V. harveyi. The major inhibitory mechanism seems to be the pH effect of organic acids. The effective concentration 50 (EC50) values at 96 h inoculation for all organic acids were determined to be 0.023, 0.041, 0.03, and 0.066% for formic, acetic, propionic, and butyric acid, respectively. The laboratory study results are encouraging to formulate shrimp feeds with organic acids to control vibrio infection in shrimp aquaculture farms.

  5. Immune defence White Spot Syndrome Virus infected shrimp, Penaeus monodon

    NARCIS (Netherlands)

    Arts, J.A.J.

    2006-01-01

    White spot syndrome virus (WSSV) is the most important viral pathogen of cultured penaeid shrimp worldwide. Since the initial discovery of the virus inTaiwanin 1992, it has spread to shrimp farming regions in Southeast Asia, the

  6. Wind effects on prey availability: How northward migrating waders use brackish and hypersaline lagoons in the sivash, Ukraine

    Science.gov (United States)

    Verkuil, Yvonne; Koolhaas, Anita; Van Der Winden, Jan

    Large numbers of waders migrating northward in spring use the Sivash, a large system of shallow, brackish and hypersaline lagoons in the Black Sea and Azov Sea region (Ukraine). The bottoms of these lagoons are often uncovered by the wind. Hence, for waders the time and space available for feeding depend on wind conditions. In hypersaline lagoons the benthic and pelagic fauna was very poor, consisting mainly of chironomid larvae (0.19 g AFDM·m -2) and brine shrimps Artemia salina, respectively. Brine shrimp abundance was correlated with salinity, wind force, wind direction and water depth. Dunlin Calidris alpina and curlew sandpiper Calidris ferruginea were the only species feeding on brine shrimp. As brine shrimp densities are higher in deeper water, smaller waders such as broad-billed sandpipers Limicola falcinellus are too short-legged to reach exploitable densities of brine shrimp. In brackish lagoons the benthic and pelagic fauna was rich, consisting of polychaetes, bivalves, gastropods, chironomid larvae, isopods and amphipods (8.9 to 30.5 g AFDM·m -2), but there were no brine shrimps. Prey biomass increased with the distance from the coast, being highest on the site that was most frequently inundated. Dunlin, broad-billed sandpiper and grey plover Pluvialis squatarola were the most abundant birds in the brackish lagoon. Due to the effects of wind-tides only a small area was usually available as a feeding site. Gammarus insensibilis was the alternative prey resource in the water layer, and their density varied with wind direction in the same way as brine shrimp. Curlew sandpipers and dunlins in the hypersaline lagoons and broad-billed sandpipers in the brackish lagoons often changed feeding sites, probably following the variation in prey availability. Only because of the large size and variety of lagoons are waders in the Sivash always able to find good feeding sites.

  7. Recreating the seawater mixture composition of HOCs in toxicity tests with Artemia franciscana by passive dosing

    Energy Technology Data Exchange (ETDEWEB)

    Rojo-Nieto, E., E-mail: elisa.rojo@uca.es [Andalusian Centre of Marine Science and Technology (CACYTMAR), Department of Environmental Technologies, University of Cadiz, 11510 Puerto Real (Spain); Smith, K.E.C. [Department of Environmental Science, Aarhus University, DK-4000 Roskilde (Denmark); Perales, J.A. [Andalusian Centre of Marine Science and Technology (CACYTMAR), Department of Environmental Technologies, University of Cadiz, 11510 Puerto Real (Spain); Mayer, P. [Department of Environmental Science, Aarhus University, DK-4000 Roskilde (Denmark)

    2012-09-15

    The toxicity testing of hydrophobic organic compounds (HOCs) in aquatic media is generally challenging, and this is even more problematic for mixtures. The hydrophobic properties of these compounds make them difficult to dissolve, and subsequently to maintain constant exposure concentrations. Evaporative and sorptive losses are highly compound-specific, which can alter not only total concentrations, but also the proportions between the compounds in the mixture. Therefore, the general aim of this study was to explore the potential of passive dosing for testing the toxicity of a PAH mixture that recreates the mixture composition found in seawater from a coastal area of Spain, the Bay of Algeciras. First, solvent spiking and passive dosing were compared for their suitability to determine the acute toxicity to Artemia franciscana nauplii of several PAHs at their respective solubility limits. Second, passive dosing was applied to recreate the seawater mixture composition of PAHs measured in a Spanish monitoring program, to test the toxicity of this mixture at different levels. HPLC analysis was used to confirm the reproducibility of the dissolved exposure concentrations for the individual PAHs and mixtures. This study shows that passive dosing has some important benefits in comparison with solvent spiking for testing HOCs in aquatic media. These include maintaining constant exposure concentrations, leading to higher reproducibility and a relative increase in toxicity. Passive dosing is also able to faithfully reproduce real mixtures of HOCs such as PAHs, in toxicity tests, reproducing both the levels and proportions of the different compounds. This provides a useful approach for studying the toxicity of environmental mixtures of HOCs, both with a view to investigating their toxicity but also for determining safety factors before such mixtures result in detrimental effects.

  8. Effects of heavy metals and pesticides on survival of Artemia franciscana

    Directory of Open Access Journals (Sweden)

    Marcel Falis

    2014-01-01

    Full Text Available Assessment of the potential risk of pesticides is an important part of registration procedures in many countries. However, risk assessment of several pesticides used during the growing season has not been carried out. The aim of this study was to investigate the effects of pesticides (azoxystrobin and glyphosate, heavy metals (cadmium chloride, potassium dichromate, zinc disulphate and their combinations on lethality to Artemia franciscana. In total, 1,250 freshly hatched nauplii of A. franciscana were used in the study. They were placed in 125 Petri dishes (10 nauplii in one Petri dish containing 10 ml of azoxystrobin, glyphosate, cadmium chloride, potassium dichromate and zinc sulphate separately, or their combinations; the control dish contained only sea water. Each experimental set consisted of 50 nauplii which were divided into five replicates of 10 nauplii per replicate. Live A. franciscana were counted after 24, 48, 72 and 96 h and the numbers were compared with the control group. A significant (P after exposure to azoxystrobin in combination with glyphosate after 48, 72 and 96 h of exposure, in combination with glyphosate and potassium dichromate after 48 h of exposure, and in combination with glyphosate and zinc disulphate after 48, 72 and 96 h of exposure. The results of this study provide information about the combined toxicity of pesticides used during the growing season and some heavy metals as major pollutants of the rural environment. A study of this kind has not been conducted yet. Further research for investigation of the combined toxicity of pollutants and pesticides is required.

  9. 78 FR 50383 - Certain Frozen Warmwater Shrimp From the Republic of Indonesia: Final Negative Countervailing...

    Science.gov (United States)

    2013-08-19

    ... Packing Co., Inc.; Lafitte Frozen Foods Corp.; M&M Shrimp (Biloxi Freezing and Processing); Ocean Springs... to as coldwater shrimp, in any state of processing; (3) fresh shrimp and prawns whether shell-on or... Facilities for LTAR 21. Government Provision of Shrimp Breeding Stock and Seed for LTAR 22. Government Loans...

  10. Influence of Thermal Preparation Method on Mineral Composition of Shrimps

    Directory of Open Access Journals (Sweden)

    Gheorghe Valentin GORAN

    2017-11-01

    Full Text Available This study goal was to evaluate the effects of 3 different cooking methods (boiling, roasting, and microwaving on mineral concentrations of shrimps from the Bucharest market. Mineral content in shrimp samples was evaluated by ICP-OES, and relative humidity was assessed by thermogravimetry. Cooking method insignificantly influenced the level of Fe. Ca and K levels were higher in cooked samples compared to raw shrimps, independent of cooking method. Essential (Cu, Se, and Zn, and non-essential and toxic (Al, Cd, Ni, and Pb elements levels were significantly increased in boiled shrimps, compared to raw and the other 2 types of cooked samples. Generally, after cooking the lowest values of essential trace elements concentration was registered in roasted samples. The highest percentage of water loss was found in boiled samples. In general, thermal preparation increased mineral concentrations in cooked samples compared to raw shrimps.

  11. Efficient use of shrimp waste: present and future trends.

    Science.gov (United States)

    Kandra, Prameela; Challa, Murali Mohan; Jyothi, Hemalatha Kalangi Padma

    2012-01-01

    The production of shrimp waste from shrimp processing industries has undergone a dramatic increase in recent years. Continued production of this biomaterial without corresponding development of utilizing technology has resulted in waste collection, disposal, and pollution problems. Currently used chemical process releases toxic chemicals such as HCl, acetic acid, and NaOH into aquatic ecosystem as byproducts which will spoil the aquatic flora and fauna. Environmental protection regulations have become stricter. Now, there is a need to treat and utilize the waste in most efficient manner. The shrimp waste contains several bioactive compounds such as chitin, pigments, amino acids, and fatty acids. These bioactive compounds have a wide range of applications including medical, therapies, cosmetics, paper, pulp and textile industries, biotechnology, and food applications. This current review article present the utilization of shrimp waste as well as an alternative technology to replace hazardous chemical method that address the future trends in total utilization of shrimp waste for recovery of bioactive compounds.

  12. Study on irradiation preservation of frozen shelled shrimps

    International Nuclear Information System (INIS)

    Liu Chunquan; Zhu Jiating; Zhao Yongfu; Yu Gang; Zhang Weidong; Jin Yudong; Ji Ping

    2004-01-01

    The effect of irradiaiton preservation of frozen shelled shrimps for export was studied. The microbial indexd, nutritional ingredient, physico-chemical index for irradiation frozen shelled shrimps were detected. The results showed that 3-5 kGy irradiation dose could kill more than 99% of all kinds of microorganisms in frozen shelled shrimps, the content of most amino acids in shelled shrimps increased, after being irradiated by 1-9 kGy dose, the total amino acids had been obvisouly higher than CK, the increased range was 0.33%-24.6%, the content of the total volatile basic nitrogen (TVBN) decreased. Compared with the CK, the content of the heavy metal elements etc had no obvious change, the presrvation duration of irradiated shelled shrimp was twelve months longer than that of CK when storage temperature was under -7 degree C soft frozen, Compared with -18 degree C the effect of irradiation preservation had no obvious change. (authors)

  13. Suppression of Shrimp Melanization during White Spot Syndrome Virus Infection*

    Science.gov (United States)

    Sutthangkul, Jantiwan; Amparyup, Piti; Charoensapsri, Walaiporn; Senapin, Saengchan; Phiwsaiya, Kornsunee; Tassanakajon, Anchalee

    2015-01-01

    The melanization cascade, activated by the prophenoloxidase (proPO) system, plays a key role in the production of cytotoxic intermediates, as well as melanin products for microbial sequestration in invertebrates. Here, we show that the proPO system is an important component of the Penaeus monodon shrimp immune defense toward a major viral pathogen, white spot syndrome virus (WSSV). Gene silencing of PmproPO(s) resulted in increased cumulative shrimp mortality after WSSV infection, whereas incubation of WSSV with an in vitro melanization reaction prior to injection into shrimp significantly increased the shrimp survival rate. The hemolymph phenoloxidase (PO) activity of WSSV-infected shrimp was extremely reduced at days 2 and 3 post-injection compared with uninfected shrimp but was fully restored after the addition of exogenous trypsin, suggesting that WSSV probably inhibits the activity of some proteinases in the proPO cascade. Using yeast two-hybrid screening and co-immunoprecipitation assays, the viral protein WSSV453 was found to interact with the proPO-activating enzyme 2 (PmPPAE2) of P. monodon. Gene silencing of WSSV453 showed a significant increase of PO activity in WSSV-infected shrimp, whereas co-silencing of WSSV453 and PmPPAE2 did not, suggesting that silencing of WSSV453 partially restored the PO activity via PmPPAE2 in WSSV-infected shrimp. Moreover, the activation of PO activity in shrimp plasma by PmPPAE2 was significantly decreased by preincubation with recombinant WSSV453. These results suggest that the inhibition of the shrimp proPO system by WSSV partly occurs via the PmPPAE2-inhibiting activity of WSSV453. PMID:25572398

  14. Suppression of shrimp melanization during white spot syndrome virus infection.

    Science.gov (United States)

    Sutthangkul, Jantiwan; Amparyup, Piti; Charoensapsri, Walaiporn; Senapin, Saengchan; Phiwsaiya, Kornsunee; Tassanakajon, Anchalee

    2015-03-06

    The melanization cascade, activated by the prophenoloxidase (proPO) system, plays a key role in the production of cytotoxic intermediates, as well as melanin products for microbial sequestration in invertebrates. Here, we show that the proPO system is an important component of the Penaeus monodon shrimp immune defense toward a major viral pathogen, white spot syndrome virus (WSSV). Gene silencing of PmproPO(s) resulted in increased cumulative shrimp mortality after WSSV infection, whereas incubation of WSSV with an in vitro melanization reaction prior to injection into shrimp significantly increased the shrimp survival rate. The hemolymph phenoloxidase (PO) activity of WSSV-infected shrimp was extremely reduced at days 2 and 3 post-injection compared with uninfected shrimp but was fully restored after the addition of exogenous trypsin, suggesting that WSSV probably inhibits the activity of some proteinases in the proPO cascade. Using yeast two-hybrid screening and co-immunoprecipitation assays, the viral protein WSSV453 was found to interact with the proPO-activating enzyme 2 (PmPPAE2) of P. monodon. Gene silencing of WSSV453 showed a significant increase of PO activity in WSSV-infected shrimp, whereas co-silencing of WSSV453 and PmPPAE2 did not, suggesting that silencing of WSSV453 partially restored the PO activity via PmPPAE2 in WSSV-infected shrimp. Moreover, the activation of PO activity in shrimp plasma by PmPPAE2 was significantly decreased by preincubation with recombinant WSSV453. These results suggest that the inhibition of the shrimp proPO system by WSSV partly occurs via the PmPPAE2-inhibiting activity of WSSV453. © 2015 by The American Society for Biochemistry and Molecular Biology, Inc.

  15. Modeling Transfer of Vibrio Parahaemolyticus During Peeling of Raw Shrimp.

    Science.gov (United States)

    Xiao, Xingning; Pang, Haiying; Wang, Wen; Fang, Weihuan; Fu, Yingchun; Li, Yanbin

    2018-03-01

    This study aimed to qualify the transfer of Vibrio parahaemolyticus during the shrimp peeling process via gloves under 3 different scenarios. The 1st 2 scenarios provided quantitative information for the probability distribution of bacterial transfer rates from (i) contaminated shrimp (6 log CFU/g) to non-contaminated gloves (Scenario 1) and (ii) contaminated gloves (6 log CFU/per pair) to non-contaminated shrimp (Scenario 2). In Scenario 3, bacterial transfer from contaminated shrimp to non-contaminated shrimp in the shrimp peeling process via gloves was investigated to develop a predictive model for describing the successive bacterial transfer. The range of bacterial transfer rate (%) in Scenarios 1 and 2 was 7% to 91.95% and 0.04% to 12.87%, respectively, indicating that the bacteria can be transferred from shrimp to gloves much easier than that from gloves to shrimp. A Logistic (1.59, 0.14) and Triangle distribution (-1.61, 0.12, 1.32) could be used to describe the bacterial transfer rate in Scenarios 1 and 2, respectively. In Scenario 3, a continuously decay patterning with fluctuations as the peeling progressed has been observed at all inoculation levels of the 1st shrimp (5, 6, and 7 log CFU/g). The bacteria could be transferred easier at 1st few peels, and the decreasing bacterial transfer was found in later phase. Two models (exponential and Weibull) could describe the successive bacterial transfer satisfactorily (pseudo-R 2 > 0.84, RMSE peeling process. The bacterial transfer rate distribution and predictive model derived from this work could be used in risk assessment of V. parahaemolyticus to ensure peeled shrimp safety. © 2018 Institute of Food Technologists®.

  16. Profitability and technical efficiency of Black tiger shrimp (Penaeus Monodon) culture and White leg shrimp (Penaeus Vannamei) culture in Song Song Cau district, Phu Yen province, Vietnam

    OpenAIRE

    Nguyen, Thi Hoai An

    2012-01-01

    The research measure the profitability and technical efficiency of Black tiger shrimp farms and White leg shrimp farms in Song Cau district, Phu Yen province, Vietnam. Cross-sectional data of 62 Black tiger shrimp samples and 88 White leg shrimp samples were used for comparison two production systems. The profitability analysis shows that White leg shrimp farms achieved an average profit per hectare of 78,883,209 VND ($3,944.16), which was approximately 4 times as much as Black tiger shrimp f...

  17. Solar desalination, brine and fine chemicals - a preliminary report

    Digital Repository Service at National Institute of Oceanography (India)

    Shirodkar, P.V.; Nagarajan, R.

    Solar stills put into operation by taking known quantities of sea water of different salinities varying from 27.75-36.27 x 10 super(3) during April-May 1990, indicated fresh water yield of 55-68% (av. 64). The volumes of brine as well as those...

  18. Durability of concrete materials in high-magnesium brine

    International Nuclear Information System (INIS)

    Wakeley, L.D.; Poole, T.S.; Burkes, J.P.

    1994-03-01

    Cement pastes and mortars representing 11 combinations of candidate concrete materials were cast in the laboratory and monitored for susceptibility to chemical deterioration in high-magnesium brine. Mixtures were selected to include materials included in the current leading candidate concrete for seals at the Waste Isolation Pilot Plant (WIPP). Some materials were included in the experimental matrix to answer questions that had arisen during study of the concrete used for construction of the liner of the WIPP waste-handling shaft. Mixture combinations compared Class C and Class F fly ashes, presence or absence of an expansive component, and presence or absence of salt as a mixture component. Experimental conditions exposed the pastes and mortars to extreme conditions, those being very high levels of Mg ion and an effectively unlimited supply of brine. All pastes and mortars showed deterioration with brine exposure. In general, mortars deteriorated more extensively than the corresponding pastes. Two-inch cube specimens of mortar were not uniformly deteriorated, but showed obvious zoning even after a year in the brine, with a relatively unreacted zone remaining at the center of each cube. Loss of calcium from the calcium hydroxide of paste/aggregate interfaces caused measurable strength loss in the reacted zone comprising the outer portion of every mortar specimen. The current candidate mass concrete for WIPP seals includes salt as an initial component, and has a relatively closed initial microstructure. Both of these features contribute to its suitability for use in large placements within the Salado Formation

  19. Brine transport in porous media self-similar solutions

    NARCIS (Netherlands)

    C.J. van Duijn (Hans); L.A. Peletier (Bert); R.J. Schotting (Ruud)

    1996-01-01

    textabstractIn this paper we analyze a model for brine transport in porous media, which includes a mass balance for the fluid, a mass balance for salt, Darcy's law and an equation of state, which relates the fluid density to the salt mass fraction. This model incorporates the effect of local volume

  20. Comparison of antimicrobial activities of brine salting, Chlorinated ...

    African Journals Online (AJOL)

    Chemical preservatives can be used to reduce the overall microbial populations in fish and fish products. This study was set to determine the antimicrobial activities of brine salting, chlorinated solution, and Moringa oleifera plant extracts treatments on enteric bacteria in Rastrineobola argentea and Oreochromis niloticus fish ...

  1. Modeling the morphogenesis of brine channels in sea ice.

    Science.gov (United States)

    Kutschan, B; Morawetz, K; Gemming, S

    2010-03-01

    Brine channels are formed in sea ice under certain constraints and represent a habitat of different microorganisms. The complex system depends on a number of various quantities as salinity, density, pH value, or temperature. Each quantity governs the process of brine channel formation. There exists a strong link between bulk salinity and the presence of brine drainage channels in growing ice with respect to both the horizontal and vertical planes. We develop a suitable phenomenological model for the formation of brine channels both referring to the Ginzburg-Landau theory of phase transitions as well as to the chemical basis of morphogenesis according to Turing. It is possible to conclude from the critical wave number on the size of the structure and the critical parameters. The theoretically deduced transition rates have the same magnitude as the experimental values. The model creates channels of similar size as observed experimentally. An extension of the model toward channels with different sizes is possible. The microstructure of ice determines the albedo feedback and plays therefore an important role for large-scale global circulation models.

  2. Solar desalination, brine and fine chemicals - a preliminary report

    Digital Repository Service at National Institute of Oceanography (India)

    Shirodkar, P.V.; Nagarajan, R.

    from 3.65 - 4.63 ppm. The definite volumes of seawater samples (3.7 litres) taken in stills for desalination correspond to 13.08 - 31.16 mg of net boron content. Analyses on the recovery of the total content of boron in brines as well as in the bitterns...

  3. [Experimental interaction of halophilic prokaryotes and opportunistic bacteria in brine].

    Science.gov (United States)

    Selivanova, E A; Nemtseva, N V

    2013-01-01

    Study the effect of extremely halophilic archaea and moderately halophilic bacteria on preservation of opportunistic bacteria in brine. 17 strains of moderately halophilic bacteria and 2 strains of extremely halophilic archaea were isolated from continental hypersaline lake Razval of Sol-Iletsk area of Orenburg Region. Identification of pure cultures of prokaryotes was carried out taking into account their phenotype properties and based on determination of 16S RNA gene sequence. The effect of halophilic prokaryote on elimination of Escherichia coli from brine was evaluated during co-cultivation. Antagonistic activity of cell extracts of the studied microorganisms was evaluated by photometric method. A more prolonged preservation of an E. coli strain in brine in the presence of live cells of extremely halophilic archaea Halorubrum tebenquichense and moderately halophilic bacteria Marinococcus halophilus was established. Extracts of cells of extremely halophilic archaea and moderately halophilic bacteria on the contrary displayed antagonistic activity. The protective effect of live cells of halophilic prokaryotes and antagonistic activity of their cell extracts change the period of conservation of opportunistic bacteria in brine that regulates inter-microbial interactions and changes the period of self-purification that reflects the sanitary condition of a hypersaline water body.

  4. A carbon inventory for Orca Basin brines and sediments

    International Nuclear Information System (INIS)

    Sackett, W.M.; Brooks, J.M.; Bernard, B.B.; Schwab, C.R.; Chung, H.; Parker, R.A.

    1979-01-01

    Orca Basin, an intraslope depression at a depth of about 2400 m on the continental slope of the north-central Gulf of Mexico, contains an anoxic, hypersaline brine similar to composition to those reported in the Red Sea. Concentrations and stable carbon isotope compositions of various inorganic and organic carbon species have been determined on the brine and sediments in order to gain an understanding of the origin and cycling of carbon in this unique environment. ΣCO 2 in the brine (55 mg C/l) is about twice seawater with delta 13 C sub(PDB)=-16.4per thousand and Δ 14 C=-501per thousand. CH 4 has a concentration of 12 mg C/l and delta 13 C=-73.5per thousand. Dissolved and particulate organic carbon concentrations are seven times higher and have delta 13 C values several permil different than the overlying seawater, ΣCO 2 and CH 4 in the interstitial waters are considerably higher in concentrations and isotropically light than the overlying brine. Solution of near-surface salt deposits by seawater with subsequent microbial production and consumption of methane can be used to explain most of the data. (Auth.)

  5. Permeability of salt-crystal interfaces to brine

    International Nuclear Information System (INIS)

    Gilpatrick, L.O.; Baes, C.F. Jr.; Shor, A.J.; Canonico, C.M.

    1982-06-01

    To investigate the movement of brine along grain boundaries in polycrystalline salt, measurements have been made of the radial flow of brine through the interface between cylindrical salt crystals under axial stresses to 140 bar and temperatures to 80 0 C. For constant conditions, the total flow of brine showed a linear dependence on the logarithm of time, and the reciprocal permeability increased linearly with time. Loss of salt from the interface by pressure solution effects was more than enough to account for the decrease in the apparent thickness of the interface (i.e., that which may be estimated for an interface of the same permeability formed by plane parallel surfaces). This apparent thickness, initially as large as 10 μm, decreased to as little as 0.2 μm with exposure to stress and flowing brine. It decreased quickly with sudden increases in axial stress and usually increased, though not reversibly, with decreases in stress. The rate of increase in the reciprocal permeability with time was roughly proportional to the stress and to the square of the hydraulic pressure drop. Assuming similar apparent thicknesses for the grain boundaries in polycrystalline salt, permeabilities are predicted that are quite consistent with the low values reported for stressed core specimens

  6. Immunization with Hypoallergens of shrimp allergen tropomyosin inhibits shrimp tropomyosin specific IgE reactivity.

    Directory of Open Access Journals (Sweden)

    Christine Y Y Wai

    Full Text Available Designer proteins deprived of its IgE-binding reactivity are being sought as a regimen for allergen-specific immunotherapy. Although shrimp tropomyosin (Met e 1 has long been identified as the major shellfish allergen, no immunotherapy is currently available. In this study, we aim at identifying the Met e 1 IgE epitopes for construction of hypoallergens and to determine the IgE inhibitory capacity of the hypoallergens. IgE-binding epitopes were defined by three online computational models, ELISA and dot-blot using sera from shrimp allergy patients. Based on the epitope data, two hypoallergenic derivatives were constructed by site-directed mutagenesis (MEM49 and epitope deletion (MED171. Nine regions on Met e 1 were defined as the major IgE-binding epitopes. Both hypoallergens MEM49 and MED171 showed marked reduction in their in vitro reactivity towards IgE from shrimp allergy patients and Met e 1-sensitized mice, as well as considerable decrease in induction of mast cell degranulation as demonstrated in passive cutaneous anaphylaxis assay. Both hypoallergens were able to induce Met e 1-recognizing IgG antibodies in mice, specifically IgG2a antibodies, that strongly inhibited IgE from shrimp allergy subjects and Met e 1-sensitized mice from binding to Met e 1. These results indicate that the two designer hypoallergenic molecules MEM49 and MED171 exhibit desirable preclinical characteristics, including marked reduction in IgE reactivity and allergenicity, as well as ability to induce blocking IgG antibodies. This approach therefore offers promises for development of immunotherapeutic regimen for shrimp tropomyosin allergy.

  7. Utilização da artêmia nacional como dieta para pós-larvas do Litopenaeus vannamei (Boone, 1931 na fase berçário - DOI: 10.4025/actascianimsci.v28i3.52 Brazilian artemia as feed for Litopenaeus vannamei post-larvae through the nursery phase - DOI: 10.4025/actascianimsci.v28i3.52

    Directory of Open Access Journals (Sweden)

    Paulo de Paula Mendes

    2006-01-01

    Full Text Available Experimentos foram realizados em uma fazenda comercial de criação de camarões marinhos, objetivando avaliar a taxa de crescimento e sobrevivência das pós-larvas do Litopenaeus vannamei, quando cultivadas em tanques-berçário e submetidas a diferentes dietas. As pós-larvas utilizadas encontravam-se com 19 dias (PL19 e foram estocadas a 16 PL/L, em tanques-berçário de 60 m3. As pós-larvas foram submetidas ao Método de Alimentação Convencional (MAC e o Método de Alimentação com Artêmia (MAA. Verificou-se que ao se correlacionar o peso em função do comprimento e do tempo de cultivo, o MAA apresentou uma relação estatisticamente superior (pExperiments were carried out in a commercial marine shrimp farm to evaluate growth and survival rate of the Litopenaeus vannamei post-larvae during nursery phase using different diets. The nursery tanks (60 m3 will be provided with post-larvae of 19 days (PL19 with a density of 16 PL/liter. The post-larvae were submitted to a Commercial Feeding Method (MAC and an Artemia Feeding Method (MAA. Considering the correlations between weight and length, and between weight and culture duration, a better efficiency (p<0.05 of the MAA was verified. The survival rate of the post-larvae fed with MAA was 86.25%, while MAC yielded survivorship of 62.12%. Thus, artemia nauplii were proved to be more efficient, providing higher growth and survival rates of Litopenaeus vannamei post-larvae

  8. Origin, distribution, and movement of brine in the Permian Basin (U.S.A.). A model for displacement of connate brine

    International Nuclear Information System (INIS)

    Bein, A.; Dutton, A.R.

    1993-01-01

    Na-Cl, halite Ca-Cl, and gypsum Ca-Cl brines with salinities from 45 to >300 g/L are identified and mapped in four hydrostratigraphic units in the Permian Basin area beneath western Texas and Oklahoma and eastern New Mexico, providing spatial and lithologic constraints on the interpretation of the origin and movement of brine. Na-Cl brine is derived from meteoric water as young as 5-10 Ma that dissolved anhydrite and halite, whereas Ca-Cl brine is interpreted to be ancient, modified-connate Permian brine that now is mixing with, and being displaced by, the Na-Cl brine. Displacement fronts appear as broad mixing zones with no significant salinity gradients. Evolution of Ca-Cl brine composition from ideal evaporated sea water is attributed to dolomitization and syndepositional recycling of halite and bittern salts by intermittent influx of fresh water and sea water. Halite Ca-Cl brine in the evaporite section in the northern part of the basin differs from gypsum Ca-Cl brine in the south-central part in salinity and Na/Cl ratio and reflects segregation between halite- and gypsum-precipitating lagoons during the Permian. Ca-Cl brine moved downward through the evaporite section into the underlying Lower Permian and Pennsylvanian marine section that is now the deep-basin brine aquifer, mixing there with pre-existing sea water. Buoyancy-driven convection of brine dominated local flow for most of basin history, with regional advection governed by topographically related forces dominant only for the past 5 to 10 Ma. 71 refs., 11 figs

  9. Stratification and space-time variability of Red Sea hot brines

    Energy Technology Data Exchange (ETDEWEB)

    Monin, A S; Plakhin, E A

    1982-11-01

    The results of hydrophysical studies in Red Sea hot brines prefaced with historical information are presented. The CTD-recorder readings show stratification of the upper brine in the Atlantis II Deep into meter-scale layers, in agreement with laboratory findings. Repeated soundings with the AIST CTD meter of the upper brine interface in the Valdivia Deep recorded internal waves of 3 to 4-h periods. The observations show the different nature of brines in the four deeps studied and the lack of contact between the brine layers of the Chain and Discovery deeps.

  10. Brine migration in salt and its implications in the geologic disposal of nuclear waste

    International Nuclear Information System (INIS)

    Jenks, G.H.; Claiborne, H.C.

    1981-12-01

    This report respresents a comprehensive review and analysis of available information relating to brine migration in salt surrounding radioactive waste in a salt repository. The topics covered relate to (1) the characteristics of salt formations and waste packages pertinent to considerations of rates, amounts, and effects of brine migration, (2) experimental and theoretical information on brine migration, and (3) means of designing to minimize any adverse effects of brine migration. Flooding, brine pockets, and other topics were not considered, since these features will presumably be eliminated by appropriate site selection and repository design. 115 references

  11. Planktonic Crustacean Culture - Live Planktonic Crustaceans as Live Feed for Finfish and Shrimps in Aquaculture

    DEFF Research Database (Denmark)

    Jepsen, Per Meyer; Syberg, Kristian; Drillet, Guillaume

    2018-01-01

    The cultivation of planktonic crustaceans as live feed is of paramount importance for the aquaculture and aquarium industries. The use of live cladocerans as feed for freshwater fish is limited to the aquarium industry, whereas Artemia and copepods are used to feed edible marine fish larvae...... assessments for hazardous chemicals. Cladocerans are widely used for ecotoxicology testing but Artemia and copepods are emerging new model species. In the present chapter, we review the culturing procedures of these important planktonic crustaceans: Artemia, cladocerans and copepods and discuss their use...

  12. Assessment of Brine Management for Geologic Carbon Sequestration

    Energy Technology Data Exchange (ETDEWEB)

    Breunig, Hanna M. [Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). Environmental Energy Technologies Division; Birkholzer, Jens T. [Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). Environmental Energy Technologies Division; Borgia, Andrea [Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). Environmental Energy Technologies Division; Price, Phillip N. [Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). Environmental Energy Technologies Division; Oldenburg, Curtis M. [Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). Environmental Energy Technologies Division; McKone, Thomas E. [Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). Environmental Energy Technologies Division

    2013-06-13

    Geologic carbon sequestration (GCS) is the injection of carbon dioxide (CO2), typically captured from stationary emission sources, into deep geologic formations to prevent its entry into the atmosphere. Active pilot facilities run by regional United States (US) carbon sequestration partnerships inject on the order of one million metric tonnes (mt) CO2 annually while the US electric power sector emits over 2000 million mt-CO2 annually. GCS is likely to play an increasing role in US carbon mitigation initiatives, but scaling up GCS poses several challenges. Injecting CO2 into sedimentary basins raises fluid pressure in the pore space, which is typically already occupied by naturally occurring, or native, brine. The resulting elevated pore pressures increase the likelihood of induced seismicity, of brine or CO2 escaping into potable groundwater resources, and of CO2 escaping into the atmosphere. Brine extraction is one method for pressure management, in which brine in the injection formation is brought to the surface through extraction wells. Removal of the brine makes room for the CO2 and decreases pressurization. Although the technology required for brine extraction is mature, this form of pressure management will only be applicable if there are cost-­effective and sustainable methods of disposing of the extracted brine. Brine extraction, treatment, and disposal may increase the already substantial capital, energy, and water demands of Carbon dioxide Capture and Sequestration (CCS). But, regionally specific brine management strategies may be able to treat the extracted water as a source of revenue, energy, and water to subsidize CCS costs, while minimizing environmental impacts. By this approach, value from the extracted water would be recovered before disposing of any resulting byproducts. Until a price is placed on carbon, we expect that utilities and other CO2 sources will be

  13. Evaluation and analysis of underground brine resources in the southern coastal area of Laizhou Bay

    Science.gov (United States)

    Tian, M.; Zhu, H. T.; Feng, J.; Zhao, Q. S.

    2016-08-01

    The southern coastal districts of Laizhou Bay are some of the most important areas for underground brine exploitation in Shandong Province. Recently, these areas have been gradually developed by the underground brine mining industry. Such economic interest has led to brine exploitation so that underground brine resources are running out. Based on this phenomenon, this study describes the supply, runoff and draining conditions of the area by collecting and organizing the background information of the studied area. Hydrogeological parameters are then calculated according to pumping tests, and the amount of sustainable resources in the coastal areas of the Southern Bank of Laizhou Bay are then calculated based on the uniform distribution of wells. Under the circumstances of underground brine mining, the exploitation potential of the underground brine is evaluated in accordance with the calculation results of exploitation quantum. Finally, suggestions are provided for the sustainable exploitation of underground brine in the area.

  14. Salted herring brine as a coating or additive for herring (Clupea harengus) products — A source of natural antioxidants?

    DEFF Research Database (Denmark)

    Albertos, Irene; Gringer, Nina; Rico, Daniel

    2016-01-01

    The objective of this study was to characterise herring brine and assess its use as natural antioxidant in herring preservation. Herring brines from different marinated products (brine from fillet-ripened spice-cured herring SC, traditional barrel-salted spice-cured herring TSp and brine from...

  15. Beyond symbiosis: cleaner shrimp clean up in culture.

    Science.gov (United States)

    Militz, Thane A; Hutson, Kate S

    2015-01-01

    Cleaner organisms exhibit a remarkable natural behaviour where they consume ectoparasites attached to "client" organisms. While this behaviour can be utilized as a natural method of parasitic disease control (or biocontrol), it is not known whether cleaner organisms can also limit reinfection from parasite eggs and larvae within the environment. Here we show that cleaner shrimp, Lysmata amboinensis, consume eggs and larvae of a harmful monogenean parasite, Neobenedenia sp., in aquaculture. Shrimp consumed parasite eggs under diurnal (63%) and nocturnal (14%) conditions as well as infectious larvae (oncomiracidia) diurnally (26%). Furthermore, we trialled the inclusion of cleaner shrimp for preventative parasite management of ornamental fish, Pseudanthias squamipinnis, and found shrimp reduced oncomiracidia infection success of host fish by half compared to controls (held without shrimp). Fish held without cleaner shrimp exhibited pigmentation changes as a result of infection, possibly indicative of a stress response. These results provide the first empirical evidence that cleaner organisms reduce parasite loads in the environment through non-symbiotic cleaning activities. Our research findings have relevance to aquaculture and the marine ornamental trade, where cleaner shrimp could be applied for prophylaxis and control of ectoparasite infections.

  16. Effect of Adding the White Legged Shrimp (Penaeus vannamei on Growth and Survival of Tiger Shrimp (Penaeus monodon in Intensive Farming

    Directory of Open Access Journals (Sweden)

    . Tarsim

    2007-12-01

    Full Text Available This experiment was carried out in the framework of a project to develop a viable shrimp polyculture technology under intensive farming. The objectives were to assess the effect of adding white legged shrimp (Penaeus vannamei on growth and survival of tiger shrimp (Penaeus monodon in intensive Farming. Tiger shrimp were stocked in 2800-3400 m2 earthen ponds at 40 individuals/m2 and reared for 133 days. White legged shrimp added at 70 day at 8±2 individuals/m2. Growth rate and total yield affected by addition of this species. Final  affected by white legged shrimp addition. No adding effects were found on the water quality parameters. The results indicate that, at densities tested, white legged shrimp addition to the intensive farming of tiger shrimp polyculture is viable as species increase crop production Key words: Penaeus vannamei, P. monodon, growth, survival, polyculture

  17. Analyzing the development of Indonesia shrimp industry

    Science.gov (United States)

    Wati, L. A.

    2018-04-01

    This research aimed to analyze the development of shrimp industry in Indonesia. Porter’s Diamond Theory was used for analysis. The Porter’s Diamond theory is one of framework for industry analysis and business strategy development. The Porter’s Diamond theory has five forces that determine the competitive intensity in an industry, namely (1) the threat of substitute products, (2) the threat of competition, (3) the threat of new entrants, (4) bargaining power of suppliers, and (5) bargaining power of consumers. The development of Indonesian shrimp industry pretty good, explained by Porter Diamond Theory analysis. Analysis of Porter Diamond Theory through four main components namely factor conditions; demand condition; related and supporting industries; and firm strategy, structure and rivalry coupled with a two-component supporting (regulatory the government and the factor of chance). Based on the result of this research show that two-component supporting (regulatory the government and the factor of chance) have positive. Related and supporting industries have negative, firm and structure strategy have negative, rivalry has positive, factor condition have positive (except science and technology resources).

  18. Determination of Contamination Profiles of Human Bacterial Pathogens in Shrimp Obtained from Java, Indonesia

    Energy Technology Data Exchange (ETDEWEB)

    Dewanti-Hariyadi, R. [Center for Assessment of Traditional Foods, Department of Food Technology and Human Nutrition, Bogor Agricultural University (Indonesia); Suliantari,; Nuraida, L. [Department of Food Technology and Human Nutrition, Faculty of Agricultural Technology, Bogor Agricultural University (Indonesia); Fardiaz, S. [Inter University for Food and Nutrition, Bogor Agricultural University, Bogor (Indonesia)

    2005-01-15

    Shrimp continues to be an important export commodity for Indonesia and contributed significantly to the country’s revenue. However, shrimp exports have been frequently rejected by importing countries due to filth, Salmonella and insanitary conditions. This study was conducted to evaluate the profiles of bacterial contamination of ocean and aquaculture shrimp obtained from the area of West, Central and East Java; frozen shrimp and shrimp during industry production of frozen shrimp. The study indicated that both ocean and aquaculture shrimp obtained from the study area were heavily contaminated. On the average, shrimp obtained from West Java were more contaminated than those obtained from East and Central Java. The total bacterial counts were generally higher in ocean shrimp than those of aquaculture ones. Salmonella was present in two of 32 samples of ocean shrimp and in four of 32 samples of aquaculture shrimp obtained from the study area. Vibrio cholerae was not detected in shrimp from West Java, but was found in three out of 16 samples obtained from East and Central Java. V. parahaemolyticus was frequently identified in aquaculture shrimp but absent in fresh ocean shrimp. Studies on shrimp collected from six sampling points during frozen shrimp production revealed that processing will reduce the number of total bacterial, E. coli, and Staphylococal counts. However, the processing did not effectively reduce the incidence of Salmonella or V. parahaemolyticus when the raw material has been contaminated with the pathogens. Sizing and grading as well as arrangement of shrimp before freezing were considered as the critical points where bacteria should be controlled to inhibit growth and cross contamination with bacteria such as Listeria. Implementation of Good Agricultural Practices in production of raw shrimp as well as Hazard Analysis Critical Control Point at the line processing are expected to improve the quality of fresh and frozen shrimp. (author)

  19. Gypsum and hydrohalite dynamics in sea ice brines

    Science.gov (United States)

    Butler, Benjamin M.; Papadimitriou, Stathys; Day, Sarah J.; Kennedy, Hilary

    2017-09-01

    Mineral authigenesis from their dissolved sea salt matrix is an emergent feature of sea ice brines, fuelled by dramatic equilibrium solubility changes in the large sub-zero temperature range of this cryospheric system on the surface of high latitude oceans. The multi-electrolyte composition of seawater results in the potential for several minerals to precipitate in sea ice, each affecting the in-situ geochemical properties of the sea ice brine system, the habitat of sympagic biota. The solubility of two of these minerals, gypsum (CaSO4 ·2H2O) and hydrohalite (NaCl · 2H2O), was investigated in high ionic strength multi-electrolyte solutions at below-zero temperatures to examine their dissolution-precipitation dynamics in the sea ice brine system. The gypsum dynamics in sea ice were found to be highly dependent on the solubilities of mirabilite and hydrohalite between 0.2 and - 25.0 ° C. The hydrohalite solubility between - 14.3 and - 25.0 ° C exhibits a sharp change between undersaturated and supersaturated conditions, and, thus, distinct temperature fields of precipitation and dissolution in sea ice, with saturation occurring at - 22.9 ° C. The sharp changes in hydrohalite solubility at temperatures ⩽-22.9 °C result from the formation of an ice-hydrohalite aggregate, which alters the structural properties of brine inclusions in cold sea ice. Favourable conditions for gypsum precipitation in sea ice were determined to occur in the region of hydrohalite precipitation below - 22.9 ° C and in conditions of metastable mirabilite supersaturation above - 22.9 ° C (investigated at - 7.1 and - 8.2 ° C here) but gypsum is unlikely to persist once mirabilite forms at these warmer (>-22.9 °C) temperatures. The dynamics of hydrohalite in sea ice brines based on its experimental solubility were consistent with that derived from thermodynamic modelling (FREZCHEM code) but the gypsum dynamics derived from the code were inconsistent with that indicated by its

  20. Modelling CO2-Brine Interfacial Tension using Density Gradient Theory

    KAUST Repository

    Ruslan, Mohd Fuad Anwari Che

    2018-03-01

    Knowledge regarding carbon dioxide (CO2)-brine interfacial tension (IFT) is important for petroleum industry and Carbon Capture and Storage (CCS) strategies. In petroleum industry, CO2-brine IFT is especially importance for CO2 – based enhanced oil recovery strategy as it affects phase behavior and fluid transport in porous media. CCS which involves storing CO2 in geological storage sites also requires understanding regarding CO2-brine IFT as this parameter affects CO2 quantity that could be securely stored in the storage site. Several methods have been used to compute CO2-brine interfacial tension. One of the methods employed is by using Density Gradient Theory (DGT) approach. In DGT model, IFT is computed based on the component density distribution across the interface. However, current model is only applicable for modelling low to medium ionic strength solution. This limitation is due to the model only considers the increase of IFT due to the changes of bulk phases properties and does not account for ion distribution at interface. In this study, a new modelling strategy to compute CO2-brine IFT based on DGT was proposed. In the proposed model, ion distribution across interface was accounted for by separating the interface to two sections. The saddle point of tangent plane distance where ( ) was defined as the boundary separating the two sections of the interface. Electrolyte is assumed to be present only in the second section which is connected to the bulk liquid phase side. Numerical simulations were performed using the proposed approach for single and mixed salt solutions for three salts (NaCl, KCl, and CaCl2), for temperature (298 K to 443 K), pressure (2 MPa to 70 MPa), and ionic strength (0.085 mol·kg-1 to 15 mol·kg-1). The simulation result shows that the tuned model was able to predict with good accuracy CO2-brine IFT for all studied cases. Comparison with current DGT model showed that the proposed approach yields better match with the experiment data

  1. Efeito alelopático e toxicidade frente à Artemia salina Leach dos extatos do fruto de Euterpe edulis Martius Allelopathic effects and toxicity against Artemia salina Leach of extracts of the fruit of Euterpe edulis Martius

    Directory of Open Access Journals (Sweden)

    Cristina Peitz de Lima

    2011-06-01

    Full Text Available Alelopatia é um processo envolvendo metabólitos secundários produzidos por plantas que infl uenciam o crescimento e desenvolvimento de sistemas agrícolas. Devido à toxicidade dos herbicidas sintéticos para o meio ambiente e para a saúde humana tem-se aumentado o interesse na exploração da alelopatia como uma alternativa para o controle de plantas daninhas. O presente trabalho avaliou efeito dos extratos dos frutos de Euterpe edulis Martius sobre o desenvolvimento de cipselas e plântulas de Lactuca sativa Linné, foram determinados o índice de velocidade de germinação, o crescimento da radícula e do hipocótilo. Para a avaliação da toxicidade dos extratos foi realizado o ensaio de toxicidade frente ao microcrustáceo Artemia salina Leach determinando-se a CL50 e percentual de mortalidade. A fração remanescente demonstrou efeito alelopático, pois todas as concentrações alteraram os valores do índice de velocidade de germinação e as concentrações de 0,2 e 0,4 mg inibiram tanto o crescimento da radícula quanto o crescimento do hipocótilo. No ensaio de toxicidade todos os extratos apresentaram CL50 superior a 1000 ppm e 0% de mortalidade das artemias, indicando a não toxicidade dos extratos.Allelopathy is a process involving secondary metabolites produced by plants that influence growth and development of agricultural systems. Because of the toxicity of synthetic herbicides to the environment and human health, there has been increased interest in exploiting allelopathy as an alternative for weed control. This study evaluated the eff ect of extracts of Euterpe edulis Martius fruits on the development of cypselae and seedlings of Lactuca sativa Linné; the germination speed index, radicle and hypocotyl growth were determined. To evaluate the toxicity of the extracts the toxicity test against Artemia salina Leach was used, where the LC50 and mortality rate were determined. Th e remaining fraction showed allelopathic effect

  2. Essential fatty acids influence metabolic rate and tolerance of hypoxia in Dover sole ( Solea solea ) larvae and juveniles

    DEFF Research Database (Denmark)

    McKenzie, David; Lund, Ivar; Pedersen, Per Bovbjerg

    2008-01-01

    Dover sole (Solea solea, Linneaus 1758) were raised from first feeding on brine shrimp (Artemia sp.) with different contents and compositions of the essential fatty acids (EFA) arachidonic acid (ARA, 20:4n - 6); eicosapentaenoic acid (EPA, 20:5n - 3), and docosahexaenoic acid (DHA, 22:6n - 3......), and their metabolic rate and tolerance to hypoxia measured prior to and following metamorphosis and settlement. Four dietary Artemia preparations were compared: (1) un-enriched; (2) enriched with a commercial EFA mixture (Easy DHA SELCO Emulsion); (3) enriched with a marine fish oil combination (VEVODAR and Incromega...... DHA) to provide a high ratio of ARA to DHA, and (4) enriched with these fish oils to provide a low ratio of ARA to DHA. Sole fed un-enriched Artemia were significantly less tolerant to hypoxia than the other dietary groups. Larvae from this group had significantly higher routine metabolic rate (RMR...

  3. Poly-ß-hydroxybutyrate content and dose of the bacterial carrier for Artemia enrichment determine the performance of giant freshwater prawn larvae.

    Science.gov (United States)

    Thai, Truong Quoc; Wille, Mathieu; Garcia-Gonzalez, Linsey; Sorgeloos, Patrick; Bossier, Peter; De Schryver, Peter

    2014-06-01

    The beneficial effects of poly-β-hydroxybutyrate (PHB) for aquaculture animals have been shown in several studies. The strategy of applying PHB contained in a bacterial carrier has, however, hardly been considered. The effect of administering PHB-accumulated Alcaligenes eutrophus H16 containing 10 or 80 % PHB on dry weight, named A10 and A80, respectively, through the live feed Artemia was investigated on the culture performance of larvae of the giant freshwater prawn (Macrobrachium rosenbergii). Feeding larvae with Artemia nauplii enriched in a medium containing 100 and 1,000 mg L(-1) A80 significantly increased the survival with about 15 % and the development of the larvae with a larval stage index of about 1 as compared to feeding non-enriched Artemia. The survival of the larvae also significantly increased with about 35 % in case of a challenge with Vibrio harveyi. The efficiency of these treatments was equal to a control treatment of Artemia enriched in an 800 mg L(-1) PHB powder suspension, while Artemia enriched in 10 mg L(-1) A80, 100 mg L(-1) A10, and 1,000 mg L(-1) A10 did not bring similar effects. From our results, it can be concluded that PHB supplemented in a bacterial carrier (i.e., amorphous PHB) can increase the larviculture efficiency of giant freshwater prawn similar to supplementation of PHB in powdered form (i.e., crystalline PHB). When the level of PHB in the bacterial carrier is high, similar beneficial effects can be achieved as crystalline PHB, but at a lower live food enrichment concentration expressed on PHB basis.

  4. Greater epitope recognition of shrimp allergens by children than by adults suggests that shrimp sensitization decreases with age.

    Science.gov (United States)

    Ayuso, Rosalía; Sánchez-Garcia, Silvia; Lin, Jing; Fu, Zhiyan; Ibáñez, María Dolores; Carrillo, Teresa; Blanco, Carlos; Goldis, Marina; Bardina, Ludmila; Sastre, Joaquín; Sampson, Hugh A

    2010-06-01

    Shellfish allergy is a long-lasting disorder typically affecting adults. Despite its high prevalence, there is limited information about allergenic shrimp proteins and the epitopes implicated in such allergic reactions. We sought to identify the IgE-binding epitopes of the 4 shrimp allergens and to characterize epitope recognition profiles of children and adults with shrimp allergy. Fifty-three subjects, 34 children and 19 adults, were selected with immediate allergic reactions to shrimp, increased shrimp-specific serum IgE levels, and positive immunoblot binding to shrimp. Study subjects and 7 nonatopic control subjects were tested by means of peptide microarray for IgE binding with synthetic overlapping peptides spanning the sequences of Litopenaeus vannamei shrimp tropomyosin, arginine kinase (AK), myosin light chain (MLC), and sarcoplasmic calcium-binding protein (SCP). The Wilcoxon test was used to determine significant differences in z scores between patients and control subjects. The median shrimp IgE level was 4-fold higher in children than in adults (47 vs 12.5 kU(A)/L). The frequency of allergen recognition was higher in children (tropomyosin, 81% [94% for children and 61% for adults]; MLC, 57% [70% for children and 31% for adults]; AK, 51% [67% for children and 21% for adults]; and SCP, 45% [59% for children and 21% for adults]), whereas control subjects showed negligible binding. Seven IgE-binding regions were identified in tropomyosin by means of peptide microarray, confirming previously identified shrimp epitopes. In addition, 3 new epitopes were identified in tropomyosin (epitopes 1, 3, and 5b-c), 5 epitopes were identified in MLC, 3 epitopes were identified in SCP, and 7 epitopes were identified in AK. Interestingly, frequency of individual epitope recognition, as well as intensity of IgE binding, was significantly greater in children than in adults for all 4 proteins. Children with shrimp allergy have greater shrimp-specific IgE antibody levels and

  5. Geochemical evolution of brines in the Salar of Uyuni, Bolivia.

    Science.gov (United States)

    Rettig, S.L.; Jones, B.F.; Risacher, F.

    1980-01-01

    Recent analyses of brines from the Salars of Uyuni and Coipasa have been compared with published data for Lakes Titicaca and Poopo to evaluate solute compositional trends in these remnants of two large Pleistocene lakes once connected by overflow from the N to the S of the Bolivian Altiplano. From Titicaca to Poopo the water shows an increase in Cl and N somewhat greater than the total solutes. Ca and SO4 increase to a lesser extent than total dissolved solids, and carbonate species are relatively constant. Between Poopo and Coipasa proportions of Ca, SO4 and CO3 continue to decrease. At Coipasa and Uyuni, the great salars frequently evaporate to halite saturation. Halite crystallization is accompanied by an increased K, Mg and SO4 in residual brines. - from Authors

  6. Insertion sequences enrichment in extreme Red sea brine pool vent

    KAUST Repository

    Elbehery, Ali H. A.

    2016-12-03

    Mobile genetic elements are major agents of genome diversification and evolution. Limited studies addressed their characteristics, including abundance, and role in extreme habitats. One of the rare natural habitats exposed to multiple-extreme conditions, including high temperature, salinity and concentration of heavy metals, are the Red Sea brine pools. We assessed the abundance and distribution of different mobile genetic elements in four Red Sea brine pools including the world’s largest known multiple-extreme deep-sea environment, the Red Sea Atlantis II Deep. We report a gradient in the abundance of mobile genetic elements, dramatically increasing in the harshest environment of the pool. Additionally, we identified a strong association between the abundance of insertion sequences and extreme conditions, being highest in the harshest and deepest layer of the Red Sea Atlantis II Deep. Our comparative analyses of mobile genetic elements in secluded, extreme and relatively non-extreme environments, suggest that insertion sequences predominantly contribute to polyextremophiles genome plasticity.

  7. Brine crude oil interactions at the oil-water interface

    DEFF Research Database (Denmark)

    Chakravarty, Krishna Hara; Fosbøl, Philip Loldrup; Thomsen, Kaj

    2015-01-01

    The impact of brine salinity and its ionic composition on oil displacement efficiency has been investigated extensively in recent years due to the potential of enhanced oil recovery (EOR). Wettability alterations through relative interactions at the mineral surface have been the basis of proposed...... in enhancing oil emulsion formation by increasing interactions between polar acids and brine solutions. The results propose the potential use of HPO42- ions in reservoirs having inactive mineral surfaces. The relative oil affinity of different ions including K+, Na+, Mg2+, and Ca2+ (cations), and Cl-, SO42...... and thus reduces the interfacial viscoelasticity of the trapped oil. These results show significant correlation between oil emulsion formation and increased oil recovery. Copyright 2015; Society of Petroleum Engineers...

  8. The determination of vanadium in brines by atomic absorption spectroscopy

    Science.gov (United States)

    Crump-Wiesner, Hans J.; Feltz, H.R.; Purdy, W.C.

    1971-01-01

    A standard addition method is described for the determination of vanadium in brines by atomic absorption spectroscopy with a nitrous oxide-acetylene flame. Sample pH is adjusted to 1.0 with concentrated hydrochloric acid and the vanadium is directly extracted with 5% cupferron in methyl isobutyl ketone (MIBK). The ketone layer is then aspirated into the flame and the recorded absorption values are plotted as a function of the concentration of the added metal. As little as 2.5 ??g l-1 of vanadium can be detected under the conditions of the procedure. Tungsten and tin interfere when present in excess of 5 and 10 ??g ml-1, respectively. The concentrations of the two interfering ions normally found in brines are well below interference levels. ?? 1971.

  9. COMPARISON OF CARBON AND NITROGEN FLUXES IN TIDEFLAT FOOD WEBS DOMINATED BY BURROWING SHRIMP OR BY CULTURED OYSTERS

    Science.gov (United States)

    Two species of indigenous, thalassinid burrowing shrimps are pests to the benthic culture of Pacific oysters (Crassostrea gigas) because deposition of sediment excavated by the shrimps buries or smothers the oysters. Carbaryl pesticide is used to reduce burrowing shrimp densitie...

  10. Elimination of Chloramphenicol by Tiger Shrimp (Penaeus monodon and White Shrimp (Litopenaeus vannamei

    Directory of Open Access Journals (Sweden)

    Heny Suseno

    2016-07-01

    Full Text Available Chloramphenicol (CAP has been illegally used in many shrimp farms in South East Asia, including Indonesia. We performed an experiment of elimination simulation of CAP in tiger shrimp (Penaeus monodon and white shrimp (Litopenaeus vannamei. After 5 days of depuration process, the concentration of CAP in P. monodon decreased to 94.85% (muscle, 97.98% (cephalothoraxes, and 90.30% (exoskeleton. The elimination half-life of CAP in P monodon was 0.596 day in the muscle, 0.716 day in cephalothorax, and 0.437 day in exoskeleton. On the other hand, concentrations of CAP in L. vannamei decreased to 97.74% (muscle, 90.30% (cephalothoraxes, and 97.63% (exoskeleton. The elimination half-life of CAP in L. vannamei was 0.6624 day (muscle, 0.859 day (cephalothorax, and 0.796 day (exoskeleton. CAP was retained better by P. monodon compared to L. vannamei.

  11. Inhibition of Weld Corrosion in Flowing Brines Containing Carbon Dioxide

    OpenAIRE

    Alawadhi, Khaled

    2009-01-01

    The aim of this research was to study the effectiveness of a typical oilfield corrosion inhibitor, which is considered to be a green inhibitor (non toxic to the environment) in controlling internal corrosion of welded X65 pipeline steel in brines saturated with carbon dioxide at one bar pressure, under dynamic flowing conditions, over a range of temperatures. Several experimental configurations were used ranging from a simple flat plate design to a novel rotating cylinder electrode, to all...

  12. Uranium, RADON and radon isotopes in selected brines of Poland

    International Nuclear Information System (INIS)

    Kozlowska, B.; Walencik, A.; Zipper, W.; Dorda, J.; Przylibski, T.A.

    2010-01-01

    Natural radioactive isotopes were studied in nine different types of brines from four locations in Poland. Investigated brines are exploited from various geological structures composed of the rocks of different chemical and mineral composition as well as different age and depth. All investigated brines are used in balneotherapy (i.e. baths, inhalations, showers). The main goal of this study was to obtain some basic knowledge on the activity range of natural elements such as uranium, RADON and radon in different brine types in Poland and their variability depending on their location in certain geological structures. Activities of 234,238 U, 226,228 Ra and 222 Rn isotopes were measured with the use of two nuclear spectrometry techniques: liquid scintillation and alpha spectrometry. The activity concentrations of 222 Rn vary from below 1 to 76.1±3.7 Bq/l, for the 226 Ra isotope from 0.19±0.01 to 85.5±0.4 Bq/l and for 228 Ra from below 0.03 to 2.17±0.09 Bq/l. For uranium isotopes, the concentrations are in the range from below 0.5 to 5.1±0.4 mBq/l for 238 U and from 1.6±0.4 to 45.6±2.0 mBq/l for 2 34U . The obtained results indicate high RADON activity concentrations corresponding to high mineralization of waters. (authors)

  13. Effect of different brine concentrations and ripening period on some ...

    African Journals Online (AJOL)

    Cheeses made from pasteurized milk (65°C for 30 min) were ripened in 11, 14 and 17 g 100 ml-1 NaCl for 90 days at 7±1°C. Some physicochemical and biochemical analyses were carried out during storage time. The effects of brine concentrations on total solids, protein, ash, salt, pH, and WSN values were found to be ...

  14. Photocatalytic reduction of nitrate using titanium dioxide for regeneration of ion exchange brine.

    Science.gov (United States)

    Yang, Ting; Doudrick, Kyle; Westerhoff, Paul

    2013-03-01

    Nitrate is often removed from groundwater by ion exchange (IX) before its use as drinking water. Accumulation of nitrate in IX brine reduces the efficiency of IX regeneration and the useful life of the regeneration brine. For the first time, we present a strategy to photocatalytically reduce nitrate in IX brine, thereby extending the use of the brine. Titanium dioxide (Evonik P90), acting as photocatalyst, reduced nitrate effectively in both synthetic brines and sulfate-removed IX brine when formic acid (FA) was used as the hole scavenger (i.e., electron donor) and the initial FA to nitrate molar ratio (IFNR) was 5.6. Increasing the NaCl level in the synthetic brine slowed the nitrate reduction rate without affecting by-product selectivity of ammonium and gaseous N species (e.g., N(2), N(2)O). In a non-modified IX brine, nitrate removal was greatly inhibited owing to the presence of sulfate, which competed with nitrate for active surface sites on P90 and induced aggregation of P90 nanoparticles. After removing sulfate through barium sulfate precipitation, nitrate was effectively reduced; approximately 3.6 × 10(24) photons were required to reduce each mole of nitrate to 83% N Gases and 17% NH(4)(+). To make optimum use of FA and control the residual FA level in treated brine, the IFNR was varied. High IFNRs (e.g., 4, 5.6) were found to be more efficient for nitrate reduction but left higher residual FA in brine. IX column tests were performed to investigate the impact of residual FA for brine reuse. The residual FA in the brine did not significantly affect the nitrate removal capacity of IX resins, and formate contamination of treated water could be eliminated by rinsing with one bed volume of fresh brine. Copyright © 2012 Elsevier Ltd. All rights reserved.

  15. When and How to Worry about OA: Greenland Shrimp Case

    DEFF Research Database (Denmark)

    Kaiser, Brooks; Ravn-Jonsen, Lars

    We use a bio-economic model of the Greenland shrimp fishery to study potential ocean acidification (OA) impacts in arctic resources. Predicted changes in pH in Baffin Bay describe an anticipated though uncertain drop. Efforts to understand changes in shrimp quantity/ quality from OA reflect...... significant uncertainty and ambiguity about direction and magnitude. Estimates on change in growth parameters show a small, statistically insignificant drop that creates expectation of only limited direct biological shifts in the bio-economic model. Indirect changes to shrimp populations in Baffin Bay from...... climate change are anticipated due to expected declines in primary production that then result in equivalent reductions in secondary production, but the range could be positive or negative. Thus, the scientific findings on the OA impacts for shrimp are sufficiently ambiguous that our results find...

  16. 2008 Economic Survey of Gulf State Shrimp License Holders

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This mail survey collected data on the economic performance of active commercial shrimp harvesters who primarily operated in inshore waters of western Florida,...

  17. Genetic diversity and historical demography of kuruma shrimp ...

    African Journals Online (AJOL)

    ... of kuruma shrimp ( Penaeus japonicus ) species complex off China based on ... of 454-bp at 5' end of mitochondrial DNA control region were conducted. ... analyses suggested a late Pleistocene population expansion for both variety I ...

  18. Sustainable shrimp farming in India - Prospects and challenges

    Digital Repository Service at National Institute of Oceanography (India)

    Achuthankutty, C.T.

    Shrimp farming has grown into a multi-crore industry in India. It has vast potential for further expansion. However, this growing industry needs to develop appropriate indigenous technologies in specific areas to make it a sustainable and profitable...

  19. Physicochemical changes in minimal ozone-treated fresh shrimp ...

    African Journals Online (AJOL)

    treated fresh shrimp were evaluated tandem with microbiological efficacy of treatment during iced storage of up to 10 days. Safely discharged from commercially available domestic-type ozone facility, a previously defined minimal ozone treatment ...

  20. Commercially important penaeid shrimp larvae in the estuaries of Goa

    Digital Repository Service at National Institute of Oceanography (India)

    Achuthankutty, C.T.

    Larval stages of the penaeid shrimps, Metapenaeus dobsoni (Miers), M. affinis (Milne - Edwards) and Penaeus merguiensis De Man were mostly distributed at the lower reaches of Mandovi and Zuari estuaries. While larvae of M. dobsoni and M. affinis...

  1. Genetic diversity of intensive cultured and wild tiger shrimp Penaeus

    African Journals Online (AJOL)

    Mahdi

    2011-02-25

    Feb 25, 2011 ... improvement of brood stock management will assist shrimp breeders to ... heterozygosity; PCR, polymerase chain reaction; MFA, most ... wild females for the supply of juveniles. ... according to the manufacturer's instruction.

  2. Design of Tomato Drying System by Utilizing Brine Geothermal

    Science.gov (United States)

    Afuar, W.; Sibarani, B.; Abdurrahman, G.; Hendrarsakti, J.

    2016-09-01

    Cultivation of tomato plants in Indonesia has been started since 1961.Tomatoes generally will rot in three days if left on storage. Moreover, low quality tomatoes have cheaper price. After harvested, tomatoes need to be treated by drying process so it can last longer. Energy for drying tomatoes can be obtained by utilizing heat from geothermal brine. Purpose of this research is to design a tomato drying system by extracting heat of geothermal brine from separator with certain flow rate to heat up water by using a heat exchanger. Furthermore, this water will be used to heat up the surrounding air which is circulated by blower system to heat up the tomatoes chamber. Tomatoes drying process needs temperature range of 50-70°C to evaporate water content from 95.7% to 26%. After that treatment, the tomatoes are expected to have better durability. The objective of this study is to determine the quantity of hot brine which is needed for drying tomatoes and to design a drying system so that tomatoes can last longer.

  3. Reverse osmosis brine for phosphorus recovery from source separated urine.

    Science.gov (United States)

    Tian, Xiujun; Wang, Guotian; Guan, Detian; Li, Jiuyi; Wang, Aimin; Li, Jin; Yu, Zhe; Chen, Yong; Zhang, Zhongguo

    2016-12-01

    Phosphorus (P) recovery from waste streams has recently been recognized as a key step in the sustainable supply of this indispensable and non-renewable resource. The feasibility of using brine from a reverse osmosis (RO) membrane unit treating cooling water as a precipitant for P recovery from source separated urine was evaluated in the present study. P removal efficiency, process parameters and precipitate properties were investigated in batch and continuous flow experiments. More than 90% of P removal was obtained from both undiluted fresh and hydrolyzed urines by mixing with RO brine (1:1, v/v) at a pH over 9.0. Around 2.58 and 1.24 Kg of precipitates could be recovered from 1 m 3 hydrolyzed and fresh urine, respectively, and the precipitated solids contain 8.1-19.0% of P, 10.3-15.2% of Ca, 3.7-5.0% of Mg and 0.1-3.5% of ammonium nitrogen. Satisfactory P removal performance was also achieved in a continuous flow precipitation reactor with a hydraulic retention time of 3-6 h. RO brine could be considered as urinal and toilet flush water despite of a marginally higher precipitation tendency than tap water. This study provides a widely available, low - cost and efficient precipitant for P recovery in urban areas, which will make P recovery from urine more economically attractive. Copyright © 2016 Elsevier Ltd. All rights reserved.

  4. Certain questions about analysis of natural gas disolved in brine

    Energy Technology Data Exchange (ETDEWEB)

    Tezuka, M; Nakamura, M; Omi, K

    1983-01-01

    The composition of the gaseous phase of stratum brines is determined and the analysis technique is described. Ordinary analysis is performed with the assumptions that the contents of small components (excluding C02 and N2) are ignorably small and that the gas contains no 02. The determined concentrations of 02 and the proportional share of N2 are calculated from results of analysis as bound with pollutants. The high sensitivity of modern analytical methods makes it possible to identify quite small and trace concentrations of components, but corrections for air contamination may partially depreciate these results. Data are cited from gas chromatographic identifications of the components of gases disolved in the stratum brines of a Japanese deposit. C2H6 is established in all samples and C3H8 in two thirds of the samples, where H2 and helium were not detected anywhere. The concentrations of the gas phase on the whole in the brine were low. 02 falls into the samples in a water dissolved state; this demands the use of unconventional proportion of 02 to N2 (1 to 3.55), which is characteristic for air and a proportion of 1 to 2 which is characteristic for disolved air. With the conventional ordinary technique the consideration of the air contamination leads to a substantial underestimate of the N2 results. At the same time, the incorrect introduction of corrections for N2 has no effect on the heat creativity of the disolved gas.

  5. Brine migration test - Asse salt mine, Federal Republic of Germany

    International Nuclear Information System (INIS)

    Rothfuchs, T.; Wieczorek, K.; Feddersen, H.K.; Staupendahl, G.; Coyle, A.J.; Kalia, H.; Eckert, J.

    1988-03-01

    This document is the final report on the Cooperative German-American 'Brine Migration Tests' that were performed at the Asse Salt Mine in the Federal Republic of Germany (FRG), the Office of Nuclear Waste Isolation (ONWI), Columbus, Ohio, and the Institut fuer Tieflagerung (IfT), Braunschweig, of the Gesellschaft fuer Strahlen- und Umweltforschung mbH Muenchen (GSF). Final test and equipment design as well as manufacturing and installation was carried out by Westinghouse Electric Corporation. The tests were designed to simulate a nuclear waste repository to measure the effects of heat and gamma radiation on brine migration, salt decrepitation, disassociation of brine, and gases collected. The thermal mechanical behavior of salt, such as room closure, stresses and changes of the properties of salt are measured and compared with predicted behavior. The performance of an array of candidate waste package materials, test equipment and procedures under repository conditions will be evaluated with a view towards future in-depth testing of potential repository sites. (orig./RB)

  6. Fluid inclusion brine compositions from Palo Duro Basin salt sites

    International Nuclear Information System (INIS)

    Moody, J.B.

    1987-01-01

    The fluid inclusion analyses were done on salt samples from Lower San Andres Cycle 4 and 5. The stable isotope composition of the fluid inclusion brines was measured on duplicate samples taken from the same fluid inclusion brine for correlation of geochemical content with the stable isotopic content. The analyzed Palo Duro Basin salt fluid inclusions are predominantly one phase, i.e., the presence of a fluid only. However, many of the larger fluid inclusions do have a small vapor bubble. This liquid/vapor ratio is so high in these vapor-containing fluid inclusions that their behavior in a thermal gradient would be almost identical to that of all liquid inclusions. Closely associated with the fluid inclusions are cryptomelane where some fibers penetrate into halite host crystal. The fluid inclusions have a wide variability in content for those components that were analyzed, even within the same salt type. The fluid inclusion brines are also acidic, ranging from 3 to 6 as measured with pH test papers

  7. CO2/Brine transport into shallow aquifers along fault zones.

    Science.gov (United States)

    Keating, Elizabeth H; Newell, Dennis L; Viswanathan, Hari; Carey, J W; Zyvoloski, G; Pawar, Rajesh

    2013-01-02

    Unintended release of CO(2) from carbon sequestration reservoirs poses a well-recognized risk to groundwater quality. Research has largely focused on in situ CO(2)-induced pH depression and subsequent trace metal mobilization. In this paper we focus on a second mechanism: upward intrusion of displaced brine or brackish-water into a shallow aquifer as a result of CO(2) injection. Studies of two natural analog sites provide insights into physical and chemical mechanisms controlling both brackish water and CO(2) intrusion into shallow aquifers along fault zones. At the Chimayó, New Mexico site, shallow groundwater near the fault is enriched in CO(2) and, in some places, salinity is significantly elevated. In contrast, at the Springerville, Arizona site CO(2) is leaking upward through brine aquifers but does not appear to be increasing salinity in the shallow aquifer. Using multiphase transport simulations we show conditions under which significant CO(2) can be transported through deep brine aquifers into shallow layers. Only a subset of these conditions favor entrainment of salinity into the shallow aquifer: high aspect-ratio leakage pathways and viscous coupling between the fluid phases. Recognition of the conditions under which salinity is favored to be cotransported with CO(2) into shallow aquifers will be important in environmental risk assessments.

  8. Job satisfaction in the shrimp trawl fisheries of Chennai, India

    OpenAIRE

    Bavinck, M.

    2012-01-01

    Shrimp trawling represents an important fishing métier in South India, generating high levels of employment and economic value. It is also a contested métier, ostensibly contributing to environmental degradation and social inequality. This paper investigates the job satisfaction of crew members (captains and workers) on board the shrimp trawlers of Chennai (former Madras). Research took place in 2007 and 2008 (N = 137). Results suggest a general satisfaction with being in the fishery. However...

  9. Green Approaches to Extract Astaxanthin from Shrimp Waste

    DEFF Research Database (Denmark)

    Razi Parjikolaei, Behnaz; Errico, Massimiliano; El-Houri, Rime Bahij

    2016-01-01

    Sunflower oil and its methyl ester have recently been shown as potential green solvents which could substitute traditional organic solvents. This study investigates the economic feasibility of using these green solvents to extract astaxanthin from shrimp processing waste. The feasibility of comme......Sunflower oil and its methyl ester have recently been shown as potential green solvents which could substitute traditional organic solvents. This study investigates the economic feasibility of using these green solvents to extract astaxanthin from shrimp processing waste. The feasibility...

  10. Price transmission in the trans-atlantic northern shrimp value chain

    DEFF Research Database (Denmark)

    Nielsen, Max; Ankamah-Yeboah, Isaac; Staahl, Lisa

    2018-01-01

    affect northern shrimp fisheries. In this paper, price transmission in the trans-Atlantic northern shrimp value chain is analysed using a Vector Auto Regressive model in Error Correction form. Cointegration, the Law of One Price (LOP) and weak exogeneity are tested. The results reveal linkages from......Recent research has found that markets for farmed shrimp and wild-caught northern shrimp in Europe are integrated, indicating that northern shrimp prices are affected by total supply and demand of shrimp. Thus, the continued growth of global aquaculture production and associated price decline...

  11. North Kalimantan is Free From EMS (Early Mortality Syndrome) in the Shrimps

    OpenAIRE

    Lestiawan, Sab; Jati, Nugroho Sasongko; Wiro, Hermas

    2014-01-01

    The acute hepatopancreatic necrosis syndrome (AHPNS) or also known worldwide as early mortility syndrome (EMS) is very dangerous for the shrimps industry since it really strikes the shrimps by larvae size. The EMS attacks shrimps at 20 to 30 days of age and at 40 days of age as well. All the shrimps that are being infected will be dead righ away. The EMS has caused the significant economic losses in the shrimps industry due to the mass mortality of the shrimps in Vietnam (in the years of 2010...

  12. Effect of iron cation on geochemical trapping of CO2 in brine

    Science.gov (United States)

    Liu, Qi; Maroto-Valer, Mercedes

    2014-05-01

    Carbon dioxide sequestration using brines has emerged as a promising technology to mitigate the adverse impacts of climate change due to its large storage capacity and favorable chemistries. However, the permanent storage (mineral trapping) of CO2 in brines takes significantly long periods of time as the formation and precipitation of carbonates is very slow .[1]. The main parameters reported to effect on mineral trapping of CO2 sequestration in brines are brine composition, brine pH, system temperature and pressure.[2, 3]. It is suggested that the precipitation of mineral carbonates is mostly dependent on brine pH. Previous studies by the authors concluded that iron in natural brines causes pH instability, but it was not ascertained whether ferric iron or ferrous iron caused pH instability .[4]. Accordingly, the aim of this project is to study synthetic brines mimicking the major ions found in natural brines and including different concentrations of ferric and ferrous iron. Three brines were prepared, as follows: Brine 1 was prepared with ferric Fe3+ iron, Brine 2 prepared with ferrous Fe2+ iron and Brine 3 prepared with no iron. A series of pH stability studies and carbonation reactions were conducted using the above three brines. It is concluded that the ferrous iron causes pH instability, while ferric iron might promote carbonate precipitation. .1. Garcia, S., et al., Sequestration of non-pure carbon dioxide streams in iron oxyhydroxide-containing saline repositories. International Journal of Greenhouse Gas Control, 2012. 7: p. 89-97. 2. Liu, Q. and M.M. Maroto-Valer, Investigation of the pH effect of a typical host rock and buffer solution on CO 2 sequestration in synthetic brines. Fuel Processing Technology, 2010. 91(10): p. 1321-1329. 3. Liu, Q. and M.M. MarotoValer, Parameters affecting mineral trapping of CO2 sequestration in brines. Greenhouse Gases: Science and Technology, 2011. 1(3): p. 211-222. 4. Druckenmiller, M.L. and M.M. Maroto-Valer, Carbon

  13. Integrated mangrove-shrimp cultivation: Potential for blue carbon sequestration.

    Science.gov (United States)

    Ahmed, Nesar; Thompson, Shirley; Glaser, Marion

    2018-05-01

    Globally, shrimp farming has had devastating effects on mangrove forests. However, mangroves are the most carbon-rich forests, with blue carbon (i.e., carbon in coastal and marine ecosystems) emissions seriously augmented due to devastating effects on mangrove forests. Nevertheless, integrated mangrove-shrimp cultivation has emerged as a part of the potential solution to blue carbon emissions. Integrated mangrove-shrimp farming is also known as organic aquaculture if deforested mangrove area does not exceed 50% of the total farm area. Mangrove destruction is not permitted in organic aquaculture and the former mangrove area in parts of the shrimp farm shall be reforested to at least 50% during a period of maximum 5 years according to Naturland organic aquaculture standards. This article reviews integrated mangrove-shrimp cultivation that can help to sequester blue carbon through mangrove restoration, which can be an option for climate change mitigation. However, the adoption of integrated mangrove-shrimp cultivation could face several challenges that need to be addressed in order to realize substantial benefits from blue carbon sequestration.

  14. STRATEGIES FOR DEVELOPING SUSTAINABLE AND COMPETITIVE CLUSTER FOR SHRIMP INDUSTRY

    Directory of Open Access Journals (Sweden)

    Anas M. Fauzi

    2012-09-01

    Full Text Available Kampung Vannamei as shrimp cluster is being developed since 2004 by PT CP Prima, tbk Surabaya through Shrimp Culture Health Management transformation technology to several traditional farmers in Gresik, Lamongan, Tuban, and Madura areas. The research objectives aims to identify and mapping of stakeholder, to analyze interaction of stakeholders, to formulate strategy from internal and external environment factors and to set priority on strategy to develop sustainable and competitive shrimp cluster in the Kampung vannamei. Primary data was collected through stakeholders’ discussion forums, questionnaires, and interviews with relevant actors. Observations to the business unit also performed to determine the production and business conditions, particularly in capturing information about the threat and challenges. While the secondary data is used in policy documents national and local area statistics, and relevant literature. Analyses were performed by using the SRI International cluster pyramid, diamond porter’s analysis, SWOT and Matrix TOWS analysis, and analytical hierarchy process. Analyses were performed by the methods discussed in qualitative and descriptive. There are 7 strategies could be implemented to develop sustainable and competitive shrimp cluster. However, it is recommended to implement the strategy base on priority, which the first priority is strategy to improve linkages between businesses in the upstream and downstream industries into multi stakeholders’ platform in shrimp industry.Keywords: Shrimp, Cluster, Competitiveness, Diamond Porter, SWOT Analysis, AHP

  15. Effect of Adding the White Legged Shrimp (Penaeus vannamei) on Growth and Survival of Tiger Shrimp (Penaeus monodon) in Intensive Farming

    OpenAIRE

    . Tarsim

    2007-01-01

    This experiment was carried out in the framework of a project to develop a viable shrimp polyculture technology under intensive farming. The objectives were to assess the effect of adding white legged shrimp (Penaeus vannamei) on growth and survival of tiger shrimp (Penaeus monodon) in intensive Farming. Tiger shrimp were stocked in 2800-3400 m2 earthen ponds at 40 individuals/m2 and reared for 133 days. White legged shrimp added at 70 day at 8±2 individuals/m2. Growth rate and total yield af...

  16. Comparative spring mechanics in mantis shrimp.

    Science.gov (United States)

    Patek, S N; Rosario, M V; Taylor, J R A

    2013-04-01

    Elastic mechanisms are fundamental to fast and efficient movements. Mantis shrimp power their fast raptorial appendages using a conserved network of exoskeletal springs, linkages and latches. Their appendages are fantastically diverse, ranging from spears to hammers. We measured the spring mechanics of 12 mantis shrimp species from five different families exhibiting hammer-shaped, spear-shaped and undifferentiated appendages. Across species, spring force and work increase with size of the appendage and spring constant is not correlated with size. Species that hammer their prey exhibit significantly greater spring resilience compared with species that impale evasive prey ('spearers'); mixed statistical results show that species that hammer prey also produce greater work relative to size during spring loading compared with spearers. Disabling part of the spring mechanism, the 'saddle', significantly decreases spring force and work in three smasher species; cross-species analyses show a greater effect of cutting the saddle on the spring force and spring constant in species without hammers compared with species with hammers. Overall, the study shows a more potent spring mechanism in the faster and more powerful hammering species compared with spearing species while also highlighting the challenges of reconciling within-species and cross-species mechanical analyses when different processes may be acting at these two different levels of analysis. The observed mechanical variation in spring mechanics provides insights into the evolutionary history, morphological components and mechanical behavior, which were not discernible in prior single-species studies. The results also suggest that, even with a conserved spring mechanism, spring behavior, potency and component structures can be varied within a clade with implications for the behavioral functions of power-amplified devices.

  17. Lethal and sublethal endpoints observed for Artemia exposed to two reference toxicants and an ecotoxicological concern organic compound.

    Science.gov (United States)

    Manfra, Loredana; Canepa, Sara; Piazza, Veronica; Faimali, Marco

    2016-01-01

    Swimming speed alteration and mortality assays with the marine crustacean Artemia franciscana were carried out. EC50 and LC50 values after 24-48h exposures were calculated for two reference toxicants, copper sulphate pentahydrate (CuSO4·5H2O) and Sodium Dodecyl Sulphate (SDS), and an ecotoxicological concern organic compound, Diethylene Glycol (DEG). Different end-points have been evaluated, in order to point out their sensitivity levels. The swimming speed alteration (SSA) was compared to mortality values and also to the hatching rate inhibition (literature data). SSA resulted to be more sensitive than the mortality and with a sensitivity comparable to (or even higher than) the hatching rate endpoint. Copyright © 2015 Elsevier Inc. All rights reserved.

  18. SEM study of diversity in the cyst surface topography of nine parthenogenetic Artemia (Crustacea: Anostraca) populations from China.

    Science.gov (United States)

    Asem, Alireza; Sun, Shi-Chun

    2014-12-01

    The cysts of nine Chinese populations of parthenogenetic Artemia were studied by scanning electron microscope. In the 270 cysts examined, 15 different morphological patterns were recognized with most of them not recorded in previous studies and the "tubercled shell surface" being the most common pattern. Results also displayed high intrapopulation variability, with the maximum of 11 patterns (in 30 cysts) recorded from the Barkol population. No positive correlation between the diversity of cyst shell patterns and ploidy compositions was found. Principal components analysis suggests higher similarity among coastal populations than among inland populations, which may be attributed to the identity of physicochemical conditions among coastal salterns and dissimilarity among inland saline lakes. © 2014 Wiley Periodicals, Inc.

  19. Pressurized brines in continental Antarctica as a possible analogue of Mars.

    Science.gov (United States)

    Forte, Emanuele; Dalle Fratte, Michele; Azzaro, Maurizio; Guglielmin, Mauro

    2016-09-12

    Interest in brines in extreme and cold environments has recently increased after they have been found on Mars. Those brines can be potential new subsurface habitats for peculiar ecosystems. In the McMurdo Dry Valleys of the Antarctic, the best analogue for Mars conditions, only a few cases of brines have been identified in some perennially frozen lakes and in one case in an underground aquifer. Here, we present the occurrence of pressurized brines in a shallow perennially ice-covered lake south of 70°S in an ice-free area of Victoria Land, Antarctica. For the first time, we also imaged, by means of ground penetrating radar data, the existence of a pingo-like-feature (PLF) formed by the extrusion of brines, which has also been confirmed by borehole evidence. Those brines are fed by an underground talik external to the lake basin, enhancing the possibility of unexploited ecosystems that could find an analogue in Martian environments.

  20. Analysis of toxicity of Anacardium occidentale L. extract submitted to ionizing radiation on embryos of Biomphalaria glabrata and Artemia salina

    International Nuclear Information System (INIS)

    Silva, Hianna A.M.F.; Sa, Jose L.F.; Lima, Claudia S.A.; Amancio, Francisco F.; Melo, Ana M.M.A.; Ribeiro, Luanna R.S.; Santos, Gustavo H.F.; Silva, Edvane B.

    2013-01-01

    The use of gamma radiation as a sterilization method for herbs, herbal medicines and foods, shows positive results regarding the retention of such products, economy and safety of the method. However, it is known that this method of processing plant material can cause chemical changes in these products related to the type of material, its components and the dose received. Evaluated, in the present study, the action of gamma radiation as a modifier of toxicity extract of Anacardium occidentale Linn. To evaluate the toxicity of the extract irradiated at doses of 5.0, 7.5 and 10.0 kGy and concentrations of 250, 500 and 1000 mg/L was used bioassays with Artemia salina and Biomphalaria glabrata. For the test to A. salina, 520 specimens were used divided into groups of 10 larvae. For the bioassay with B. glabrata, 3900 specimens were used divided into groups of, approximately, 100 embryos. Larvae of A. salina and embryos were subjected to extracts irradiated and unirradiated for 24 hours. The bioassay with A. salina, showed a decrease, compared to extract unirradiated and irradiated at doses of 5.0 and 7.5 kGy, of extract irradiated with 10 kGy, where the mortality did not differ from the control group. In tests with embryos was observed an increase in the toxicity of the extract at a dose of 7.5 kGy and a decrease in the dose of 10.0 kGy. The radiation promoted changes in the toxicity of leaves extracts of Anacardium occidentale Linn. on embryos of Biomphalaria glabrata and Artemia salina. (author)

  1. Analysis of toxicity of Anacardium occidentale L. extract submitted to ionizing radiation on embryos of Biomphalaria glabrata and Artemia salina

    Energy Technology Data Exchange (ETDEWEB)

    Silva, Hianna A.M.F.; Sa, Jose L.F.; Lima, Claudia S.A.; Amancio, Francisco F.; Melo, Ana M.M.A., E-mail: hiannaamfs@gmail.com, E-mail: luismuma6@gmail.com, E-mail: claudia.salima@gmail.com, E-mail: amancioff@bol.com.br, E-mail: amdemelo@hotmail.com [Universidade Federal de Pernambuco (UFPE), Recife, PE (Brazil). Departamento de Biofisica e Radiobiologia; Ribeiro, Luanna R.S.; Santos, Gustavo H.F.; Silva, Edvane B., E-mail: luannaribeiro_lua@hotmail.com, E-mail: santosghf@hotmail.com, E-mail: edvborges@yahoo.com.br [Universidade Federal de Pernambuco (UFPE), Recife, PE (Brazil). Departamento de Energia Nuclear. Grupo de Estudos em Radioprotecao e Radioecologia

    2013-07-01

    The use of gamma radiation as a sterilization method for herbs, herbal medicines and foods, shows positive results regarding the retention of such products, economy and safety of the method. However, it is known that this method of processing plant material can cause chemical changes in these products related to the type of material, its components and the dose received. Evaluated, in the present study, the action of gamma radiation as a modifier of toxicity extract of Anacardium occidentale Linn. To evaluate the toxicity of the extract irradiated at doses of 5.0, 7.5 and 10.0 kGy and concentrations of 250, 500 and 1000 mg/L was used bioassays with Artemia salina and Biomphalaria glabrata. For the test to A. salina, 520 specimens were used divided into groups of 10 larvae. For the bioassay with B. glabrata, 3900 specimens were used divided into groups of, approximately, 100 embryos. Larvae of A. salina and embryos were subjected to extracts irradiated and unirradiated for 24 hours. The bioassay with A. salina, showed a decrease, compared to extract unirradiated and irradiated at doses of 5.0 and 7.5 kGy, of extract irradiated with 10 kGy, where the mortality did not differ from the control group. In tests with embryos was observed an increase in the toxicity of the extract at a dose of 7.5 kGy and a decrease in the dose of 10.0 kGy. The radiation promoted changes in the toxicity of leaves extracts of Anacardium occidentale Linn. on embryos of Biomphalaria glabrata and Artemia salina. (author)

  2. Defense systems in developing Artemia franciscana nauplii and their modulation by probiotic bacteria offer protection against a Vibrio anguillarum challenge.

    Science.gov (United States)

    Giarma, Eleni; Amanetidou, Eleni; Toufexi, Alexia; Touraki, Maria

    2017-07-01

    The alterations of immune responses of Artemia franciscana nauplii as a function of culture time and after a challenge with the pathogen Vibrio anguillarum were studied. The effect of the administration of the probiotic bacteria Bacillus subtilis, Lactobacillus plantarum and Lactococcus lactis either alone or in combination with the pathogen was evaluated. The activity of the antioxidant enzymes superoxide dismutase (SOD), Glutathione reductase (GRed), Glutathione transferase (GST) and Phenoloxidase (PO) presented a significant increase as a function of culture time, appeared elevated following probiotic administration and were depleted 48 h following the experimental challenge. Lipid peroxidation reached peak levels at 48 h of culture, when nauplii start feeding and returned to lower values at 144 h, remaining however significantly higher than control (P probiotics significantly reduced lipid peroxidation in comparison with the corresponding control, while challenge with the pathogen resulted in its threefold increase. Survival of nauplii remained high throughout culture and was either increased or remained at control levels following the administration of the probiotics. The challenge with the pathogen resulted in a significantly decreased survival of 15.3% for the positive control, while in the probiotic treated series survival values were not significantly different from the negative control (P > 0.05). Following a combined administration of each probiotic and the pathogen the activities of all enzymes tested were significantly lower (P probiotic). Lipid peroxidation was significantly lower in the probiotic treated series in comparison to the positive control (P probiotics B. subtilis, L. plantarum and L. lactis protect Artemia against a V. anguillarum challenge by enhancing its immune responses thus contributing to reduced oxidative damage and increased survival. Copyright © 2017 Elsevier Ltd. All rights reserved.

  3. The technology of uranium extraction from the brine with high chlorine-ion content

    International Nuclear Information System (INIS)

    Khakimov, N.; Nazarov, Kh.M.; Mirsaidov, I.U.; Negmatov, Sh.I.; Barotov, B.B.

    2010-01-01

    Present article is devoted to technology of uranium extraction from the brine with high chlorine-ion content. The research results on uranium extraction from the brine of Sasik-Kul Lake by means of sorption method were considered. The chemical composition of salt was determined. The process of uranium sorption was described and analyzed. The technology of uranium extraction from the brine with high chlorine-ion content was proposed.

  4. Pressure-induced brine migration in consolidated salt in a repository

    International Nuclear Information System (INIS)

    Hwang, Y.; Chambre, P.L.; Lee, W.W.L.; Pigford, T.H.

    1987-06-01

    This report describes a mathematical model for brine migration through intact salt near a radioactive waste package emplaced in salt. Solutions indicate limited movement following ten years emplacement

  5. Pressurized brines in continental Antarctica as a possible analogue of Mars

    OpenAIRE

    Forte, Emanuele; Dalle Fratte, Michele; Azzaro, Maurizio; Guglielmin, Mauro

    2016-01-01

    Interest in brines in extreme and cold environments has recently increased after they have been found on Mars. Those brines can be potential new subsurface habitats for peculiar ecosystems. In the McMurdo Dry Valleys of the Antarctic, the best analogue for Mars conditions, only a few cases of brines have been identified in some perennially frozen lakes and in one case in an underground aquifer. Here, we present the occurrence of pressurized brines in a shallow perennially ice-covered lake sou...

  6. Strontium isotope evidence on the history of oilfield brines, Mediterranean Coastal Plain, Israel

    International Nuclear Information System (INIS)

    Starinsky, A.; Bielski, M.; Lazar, B.; Steinitz, G.; Raab, M.

    1983-01-01

    The isotopic composition of Sr in oil field brines from the Mediterranean Coastal Plain was determined in 18 drillholes. The brines are characterized by salinities ranging from 35 to 93 g/l (TDS), Sr from 28 to 350 mg/l, Sr/Ca molar ratios from 0.011 to 0.053 and 87 Sr/ 86 Sr ratios from 0.7075 to 0.7090. E and A = 0.7081 +- 0.0004 (2σ). The brines are classified into two groups: (a) Mavqi'im group - brines with relatively high 87 Sr/ 86 Sr ratios, sampled from clastics, dolomites and anhydrites of Upper Miocene age. (b) Heletz group - brines with relatively low 87 Sr/ 86 Sr ratios, sampled from sandstones and dolomites of Lower Cretaceous age. Equations were derived to show the relations between 87 Sr/ 86 Sr ratio of the brines and the processes through which they evolved. It is suggested that both groups of brines originated from Mediterranean evaporated seawater during the Messinian desiccation. The strontium isotope composition of the seawater is reflected in that of both groups of brines, the Mavqi'im group containing the original 87 Sr/ 86 Sr ratio. The Heletz group evolved later on, through exchange reactions of those primary brines with a carbonate sequence of Cretaceous age and consequently new 87 Sr/ 86 Sr ratios could have been developed. (author)

  7. Brine migrations in the Athabasca Basin platform, alteration and associated fluid-rock exchanges

    International Nuclear Information System (INIS)

    Mercadier, J.; Cathelineau, M.; Richard, A.; Boiron, M.Ch.; Cuney, M.; Milesi, J.P.

    2009-01-01

    Uranium deposits of Athabasca Basin (Saskatchewan, Canada) are considered as the richest in the world. They result from massive percolation of basin brines in the underlying platform. The authors describe the brine movements and how structures and micro-fractures promoted this percolation until very important depths (hundreds of meters under the discordance), and their chemical modifications as they interacted with platform rocks, thus promoting the transformation of an initially sodic brine into a uranium-enriched calcic brine which is essential to the formation of discordance-type deposit

  8. RESPONSE OF GHOST SHRIMP (NEOTRYPAEA CALIFORNIENSIS) BIOTURBATION TO ORGANIC MATTER ENRICHMENT OF ESTUARINE INTERTIDAL SEDIMENTS

    Science.gov (United States)

    Populations of burrowing shrimp (Neotrypaea californiensis and Upogebia p;ugettensis) are the dominant invertebrate fauna on Pacific estuarine tide flats, occupying >80% of intertidal area in some estuaries. Burrowing shrimp are renowned for their bioturbation of intertidal sedi...

  9. 78 FR 33347 - Certain Frozen Warmwater Shrimp From Ecuador: Preliminary Negative Countervailing Duty Determination

    Science.gov (United States)

    2013-06-04

    ... States (``HTSUS''), are products which are processed from warmwater shrimp and prawns through freezing..., spices or sauce are included in the scope. In addition, food preparations (including dusted shrimp...

  10. Simulating environmental effects on brown shrimp production in the northern Gulf of Mexico

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Brown shrimp (Farfantepenaeus aztecus) are a commercially important fishery species of the northern Gulf of Mexico. Young shrimp settle in estuarine salt marsh...

  11. Economic Data Collection for Gulf of Mexico South Atlantic Shrimp Fisheries

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Annual Economic Survey of Federal Gulf and Atlantic Shrimp Permit Holders collects data about operating expenses and costs of owning and maintaining shrimp...

  12. Use of slaughter house waste as a feed for shrimps and prawns

    Digital Repository Service at National Institute of Oceanography (India)

    Sumitra-Vijayaraghavan; Royan, J.P.; Krishnakumari, L.

    Feeding experiments with some shrimps and fishes (Metapenaeus monoceros, Metapenaeus dobsoni, Sarotherodon mossambicus and Etroplus suratensis) using slaughter house waste as food, showed that the shrimps and arotherodon were able to utilize...

  13. Effect of irradiation on nutrient quality of the frozen shelled shrimp

    International Nuclear Information System (INIS)

    Fu Junjie; Liu Bojing

    2004-01-01

    The effects of irradiation on nutrient quality including amino acids, protein, fatty acids, Vitamin A, and Vitamin E in the refrigerated shelled sea shrimp and river shrimp were studied. The results showed that there was no significant change on protein, fatty acids and amino acids of irradiation 2kGy at shrimps compared with the non-irradiated shrimp. Vitamin A, and Vitamin E were decreased with the increasing of dose

  14. Impact of value chain governance on the development of small scale shrimp farmers in Vietnam

    OpenAIRE

    T. M. H. Ho; P. Burny

    2016-01-01

    Purpose of this paper is to identify the tendency of shrimp value chain development and impact of its governance on the small scale shrimp farmers in Vietnam. Design/methodology/approach - Data from the shrimp farmers surveys in Mekong delta, Vietnam from 2008-2010 with the update information in 2014 were taken to analyse by the value chain analysis method. Findings – Traditional governance type of the shrimp value chain in the early state (before 2004) showed the different levels of coordina...

  15. Impact of value chain governance on the development of small scale shrimp farmers in Vietnam

    OpenAIRE

    Ho Thi Minh, Hop; Burny, Philippe

    2016-01-01

    Purpose of this paper is to identify the tendency of shrimp value chain development and impact of its governance on the small scale shrimp farmers in Vietnam. Design/methodology/approach - Data from the shrimp farmers surveys in Mekong delta, Vietnam from 2008-2010 with the update information in 2014 were taken to analyse by the value chain analysis method. Findings – Traditional governance type of the shrimp value chain in the early state (before 2004) showed the different levels of co...

  16. Global and local governance of shrimp farming in the Mekong Delta, Vietnam

    OpenAIRE

    Tran Thi Thu, H.

    2012-01-01

    The Mekong Delta is one of seven ecological regions in Vietnam where aquaculture and shrimp products are internationally traded and the shrimp farmers are firmly embedded in a global system of production and trade. The growth of shrimp aquaculture, in addition to population growth and higher levels of investment, has left coastal resources in the Mekong Delta increasingly vulnerable to rapid changes in land and resource use. The shrimp industry, made up of multiple stakeholders and fragmente...

  17. TOUGHREACT Testing in High Ionic Strength Brine Sandstone Systems

    International Nuclear Information System (INIS)

    Xu, Tianfu

    2008-01-01

    Deep saline formations and oil and gas reservoirs often contain concentrated brine solutions of ionic strength greater than 1 (I > 1 M). Geochemical modeling, involving high ionic strength brines, is a challenge. In the original TOUGHREACT code (Xu et al., 2004; Xu et al., 2006), activity coefficients of charged aqueous species are computed using an extended Debye-Huckel (DH) equation and parameters derived by Helgeson et al. (1981). The DH model can deal with ionic strengths from dilute to moderately saline water (up to 6 molal for an NaCl-dominant solution). The equations implemented for the DH model are presented in Appendix A. During the course of the Yucca Mountain project, a Pitzer ion-interaction model was implemented into TOUGHREACT. This allows the application of this simulator to problems involving much more concentrated aqueous solutions, such as those involving geochemical processes in and around high-level nuclear waste repositories where fluid evaporation and/or boiling is expected to occur (Zhang et al., 2007). The Pitzer ion-interaction model, which we refer to as the Pitzer virial approach, and associated ion-interaction parameters have been applied successfully to study non-ideal concentrated aqueous solutions. The formulation of the Pitzer model is presented in Appendix B; detailed information can be founded in Zhang et al. (2007). For CO 2 geological sequestration, the Pitzer ion-interaction model for highly concentrated brines was incorporated into TOUGHREACT/ECO2N, then was tested and compared with a previously implemented extended Debye-Hueckel (DH) ion activity model. The comparison was made through a batch geochemical system using a Gulf Coast sandstone saline formation

  18. Radiation chemistry of salt-mine brines and hydrates

    International Nuclear Information System (INIS)

    Jenks, G.H.; Walton, J.R.; Bronstein, H.R.; Baes, C.F. Jr.

    1981-07-01

    Certain aspects of the radiation chemistry of NaCl-saturated MgCl 2 solutions and MgCl 2 hydrates at temperatures in the range of 30 to 180 0 C were investigated through experiments. A principal objective was to establish the values for the yields of H 2 [G(H 2 )] and accompanying oxidants in the gamma-ray radiolysis of concentrated brines that might occur in waste repositories in salt. We concluded that G(H 2 ) from gamma-irradiated brine solution into a simultaneously irradiated, deaerated atmosphere above the solution is between 0.48 and 0.49 over most of the range 30 to 143 0 C. The yield is probably somewhat lower at the lower end of this range, averaging 0.44 at 30 to 45 0 C. Changes in the relative amounts of MgCl 2 and NaCl in the NaCl-saturated solutions have negligible effects on the yield. The yield of O 2 into the same atmosphere averages 0.13, independent of the temperature and brine composition, showing that only about 50% of the radiolytic oxidant that was formed along with the H 2 was present as O 2 . We did not identify the species that compose the remainder of the oxidant. We concluded that the yield of H 2 from a gamma-irradiated brine solution into a simultaneously irradiated atmosphere containing 5 to 8% air in He may be greater than the yield in deaerated systems by amounts ranging from 0% for temperatures of 73 to 85 0 C, to about 30 and 40% for temperatures in the ranges 100 to 143 0 C and 30 to 45 0 C, respectively. We did not establish the mechanism whereby the air affected the yields of H 2 and O 2 . The values found in this work for G(H 2 ) in deaerated systems are in approximate agreement with the value of 0.44 for the gamma-irradiation yield of H 2 in pure H 2 O at room temperature. They are also in agreement with the values predicted by extrapolation from the findings of previous researchers for the value for G(H 2 ) in 2 M NaCl solutions at room temperature

  19. Modeling of nonuniform corrosion in salt brines: Salt Repository Project

    International Nuclear Information System (INIS)

    Reimus, P.W.

    1988-03-01

    A mechanistic approach to modeling nonuniform corrosion in brines is presented in this report. Equations are derived for completely describing the electrochemical environment within a localized corrosion cavity, and appropriate initial and boundary conditions are invoked to obtain a solvable system of equations. The initial and boundary conditions can be adjusted to simulate pitting, crevice corrosion, or stress corrosion cracking. Although no numerical results are presented, a numerical strategy for solving the equations is presented. The report focuses on the nonuniform corrosion behavior of mild steel; however, the modeling approach presented is expected to apply to a broad range of metallic materials. 34 refs., 5 figs., 2 tabs

  20. Protein removal from waste brines generated during ham salting through acidification and centrifugation.

    Science.gov (United States)

    Gutiérrez-Martínez, Maria del Rosario; Muñoz-Guerrero, Hernán; Alcaína-Miranda, Maria Isabel; Barat, José Manuel

    2014-03-01

    The salting step in food processes implies the production of large quantities of waste brines, having high organic load, high conductivity, and other pollutants with high oxygen demand. Direct disposal of the residual brine implies salinization of soil and eutrophication of water. Since most of the organic load of the waste brines comes from proteins leaked from the salted product, precipitation of dissolved proteins by acidification and removal by centrifugation is an operation to be used in waste brine cleaning. The aim of this study is optimizing the conditions for carrying out the separation of proteins from waste brines generated in the pork ham salting operation, by studying the influence of pH, centrifugal force, and centrifugation time. Models for determining the removal of proteins depending on the pH, centrifugal force, and time were obtained. The results showed a high efficacy of the proposed treatment for removing proteins, suggesting that this method could be used for waste brine protein removal. The best pH value to be used in an industrial process seems to be 3, while the obtained results indicate that almost 90% of the proteins from the brine can be removed by acidification followed by centrifugation. A further protein removal from the brine should have to be achieved using filtrating techniques, which efficiency could be highly improved as a consequence of the previous treatment through acidification and centrifugation. Waste brines from meat salting have high organic load and electrical conductivity. Proteins can be removed from the waste brine by acidification and centrifugation. The total protein removal can be up to 90% of the initial content of the waste brine. Protein removal is highly dependent on pH, centrifugation rate, and time. © 2014 Institute of Food Technologists®

  1. Strong population genetic structure and larval dispersal capability of the burrowing ghost shrimp (Neotrypaea californiensis)

    Science.gov (United States)

    The burrowing ghost shrimp, Neotrypaea californiensis, is a vital member of the estuarine benthic community. Dense populations of shrimp are found in the major estuaries of Washington and Oregon. Our study determines the genetic structure of shrimp populations in order to gain ...

  2. Analysis of Salmonella sp bacterial contamination on Vannamei Shrimp using binary logit model approach

    Science.gov (United States)

    Oktaviana, P. P.; Fithriasari, K.

    2018-04-01

    Mostly Indonesian citizen consume vannamei shrimp as their food. Vannamei shrimp also is one of Indonesian exports comodities mainstay. Vannamei shrimp in the ponds and markets could be contaminated by Salmonella sp bacteria. This bacteria will endanger human health. Salmonella sp bacterial contamination on vannamei shrimp could be affected by many factors. This study is intended to identify what factors that supposedly influence the Salmonella sp bacterial contamination on vannamei shrimp. The researchers used the testing result of Salmonella sp bacterial contamination on vannamei shrimp as response variable. This response variable has two categories: 0 = if testing result indicate that there is no Salmonella sp on vannamei shrimp; 1 = if testing result indicate that there is Salmonella sp on vannamei shrimp. There are four factors that supposedly influence the Salmonella sp bacterial contamination on vannamei shrimp, which are the testing result of Salmonella sp bacterial contamination on farmer hand swab; the subdistrict of vannamei shrimp ponds; the fish processing unit supplied by; and the pond are in hectare. This four factors used as predictor variables. The analysis used is Binary Logit Model Approach according to the response variable that has two categories. The analysis result indicates that the factors or predictor variables which is significantly affect the Salmonella sp bacterial contamination on vannamei shrimp are the testing result of Salmonella sp bacterial contamination on farmer hand swab and the subdistrict of vannamei shrimp ponds.

  3. 75 FR 44229 - Certain Frozen Warmwater Shrimp from India: Preliminary Results of Antidumping Duty Changed...

    Science.gov (United States)

    2010-07-28

    ... shrimp and prawns through freezing and which are sold in any count size. The products described above may... are packed with marinade, spices or sauce are included in the scope of this order. In addition, food... to IQF freezing immediately after application of the dusting layer. Battered shrimp is a shrimp-based...

  4. 78 FR 50385 - Certain Frozen Warmwater Shrimp from India: Final Affirmative Countervailing Duty Determination

    Science.gov (United States)

    2013-08-19

    ... Shrimp Co.; JBS Packing Co., Inc.; Lafitte Frozen Foods Corp.; M&M Shrimp (Biloxi Freezing and Processing... prawns through freezing and which are sold in any count size. The products described above may be..., spices or sauce are included in the scope. In addition, food preparations (including dusted shrimp...

  5. 75 FR 51756 - Certain Frozen Warmwater Shrimp From India: Notice of Rescission of Antidumping Duty Changed...

    Science.gov (United States)

    2010-08-23

    ... shrimp and prawns through freezing and which are sold in any count size. The products described above may... are packed with marinade, spices or sauce are included in the scope of this order. In addition, food... subjected to IQF freezing immediately after application of the dusting layer. Battered shrimp is a shrimp...

  6. Livelihood Capabilities and Pathways of Shrimp Farmers in the Mekong Delta, Vietnam

    NARCIS (Netherlands)

    Tran Thi Phung Ha, Ha; Dijk, van J.W.M.; Bosma, R.H.; Sinh, L.X.

    2013-01-01

    Shrimp farming is a major livelihood activity in the Mekong Delta in the southernmost part of Vietnam. The Vietnamese government has promoted shrimp farming as a way to reduce poverty, provide employment opportunities and increase exports to support economic development. The shrimp farming system,

  7. Global and local governance of shrimp farming in the Mekong Delta, Vietnam

    NARCIS (Netherlands)

    Tran Thi Thu, H.

    2012-01-01

    The Mekong Delta is one of seven ecological regions in Vietnam where aquaculture and shrimp products are internationally traded and the shrimp farmers are firmly embedded in a global system of production and trade. The growth of shrimp aquaculture, in addition to population growth and higher

  8. 75 FR 48724 - Frozen Warmwater Shrimp From Brazil, China, India, Thailand, and Vietnam

    Science.gov (United States)

    2010-08-11

    ... Warmwater Shrimp From Brazil, China, India, Thailand, and Vietnam AGENCY: United States International Trade... warmwater shrimp from Brazil, China, India, Thailand, and Vietnam. SUMMARY: The Commission hereby gives... warmwater shrimp from Brazil, China, India, Thailand, and Vietnam would be likely to lead to continuation or...

  9. 75 FR 57501 - Frozen Warmwater Shrimp From Brazil, China, India, Thailand, and Vietnam

    Science.gov (United States)

    2010-09-21

    ... Warmwater Shrimp From Brazil, China, India, Thailand, and Vietnam AGENCY: United States International Trade... warmwater shrimp from Brazil, China, India, Thailand, and Vietnam. SUMMARY: The Commission hereby gives... warmwater shrimp from Brazil, China, India, Thailand, and Vietnam would be likely to lead to continuation or...

  10. 75 FR 22424 - Frozen Warmwater Shrimp From Brazil, China, India, Thailand, and Vietnam

    Science.gov (United States)

    2010-04-28

    ... Warmwater Shrimp From Brazil, China, India, Thailand, and Vietnam AGENCY: United States International Trade... antidumping duty orders on frozen warmwater shrimp from Brazil, China, India, Thailand, and Vietnam. SUMMARY... duty orders on frozen warmwater shrimp from Brazil, China, India, Thailand, and Vietnam would be likely...

  11. 77 FR 36998 - Proposed Information Collection; Comment Request; Mandatory Shrimp Vessel and Gear...

    Science.gov (United States)

    2012-06-20

    ... Collection; Comment Request; Mandatory Shrimp Vessel and Gear Characterization Survey AGENCY: National... under the Shrimp Fishery Management Plan (FMP). The regulations for the Gulf Shrimp Vessel and Gear... and Gear Characterization Form. The form will be provided by NMFS at the time of permit application...

  12. 78 FR 40436 - Proposed Information Collection; Comment Request; Mandatory Shrimp Vessel and Gear...

    Science.gov (United States)

    2013-07-05

    ... Collection; Comment Request; Mandatory Shrimp Vessel and Gear Characterization Survey AGENCY: National... under the Shrimp Fishery Management Plan (FMP). The regulations for the Gulf Shrimp Vessel and Gear... Gear Characterization Form. The form will be provided by NMFS at the time of permit application and...

  13. Transmission of white spot syndrome virus (WSSV) from Dendronereis spp. (Peters) (Nereididae) to penaeid shrimp

    NARCIS (Netherlands)

    Haryadi, D.; Verreth, J.A.J.; Verdegem, M.C.J.; Vlak, J.M.

    2015-01-01

    Dendronereis spp. (Peters) (Nereididae) is a common polychaete in shrimp ponds built on intertidal land and is natural food for shrimp in traditionally managed ponds in Indonesia. White spot syndrome virus (WSSV), an important viral pathogen of the shrimp, can replicate in this polychaete (Desrina

  14. 75 FR 22370 - Certain Frozen Warmwater Shrimp From Brazil, Ecuador, India, the People's Republic of China...

    Science.gov (United States)

    2010-04-28

    ...-893, A-549-822, A-552-802] Certain Frozen Warmwater Shrimp From Brazil, Ecuador, India, the People's... certain frozen warmwater shrimp from Brazil, Ecuador, India, the People's Republic of China, Thailand, and... Frozen and Canned Warmwater Shrimp From Ecuador, 69 FR 76913 (December 23, 2004) (Ecuador Final...

  15. 78 FR 50381 - Certain Frozen Warmwater Shrimp From Malaysia: Final Affirmative Countervailing Duty Determination

    Science.gov (United States)

    2013-08-19

    ... Corp.; M&M Shrimp (Biloxi Freezing and Processing); Ocean Springs Seafood Market, Inc.; Paul Piazza... to as coldwater shrimp, in any state of processing; (3) fresh shrimp and prawns whether shell-on or... Zones 12. Provision of Seed and Fry for LTAR 13. Loans Under the Fund for Food Program 14. Loans Under...

  16. Comparison of bioassays using the anostracan crustaceans Artemia salina and Thamnocephalus platyurus for plant extract toxicity screening Comparação de bioensaios com os crustáceos Artemia salina e Thamnocephalus platyurus para abordagem de extratos de plantas com toxicidade

    Directory of Open Access Journals (Sweden)

    Pablo Mayorga

    2010-12-01

    Full Text Available Three lethality bioassays, using the salt-water crustacean Artemia salina Leach, Artemiidae, (conventional 96 microwell plate test and the Artoxkit M microbiotest and the freshwater crustacean Thamnocephalus platyurus Packard, Thamnocephalidae, (Thamnotoxkit F microbiotest, were compared using extracts of ten Guatemalan plant species. It was previously observed that five of them have anti-Artemia activity. These were: Solanum americanum Mill., Solanaceae, Gliricidia sepium (Jacq. Kunth ex Walp., Fabaceae, Neurolaena lobata (L. Cass., Asteraceae, Petiveria alliacea L., Phytolaccaceae, and Ocimum campechianum Mill., Lamiaceae. The five others: Curatella americana L., Dilleniaceae, Prunus barbata Koehne, Rosaceae, Quercus crispifolia Trel., Fagaceae, Rhizophora mangle L., Rhizophoraceae, and Smilax domingensis Willd., Smilacaceae, do not. All plants without anti-Artemia activity had no lethal effects in both assays with A. salina. For the plants with anti-Artemia activity the Artoxkit M was not sensitive to G. sepium and the conventional Artemia test was not sensitive to S. americanum, G. sepium and N. lobata. All the plant extracts, except for that of C. americana, had lethal effects on T. platyurus and the lethal median concentration (LC50 levels for this organism were in all cases substantially lower than those of the salt-water test species. This study revealed that T. platyurus is a promising test species worth further in depth investigation for toxicity screening of plant extracts with potential medicinal properties.Três bioensaios de letalidade com o crustáceo de água salgada Artemia salina Leach, Artemiidae, (teste convencional em microplaca de 96 poós Artoxkit microbiotest M e o crustáceo de água doce Thamnocephalus platyurus Packard, Thamnocephalidae (Thamnotoxkit microbiotest F, foram comparados utilizando extratos de dez espécies de plantas da Guatemala. Foi previamente observado que cinco delas possuem atividade anti-Artemia

  17. IMPROVED PRODUCTION OF TIGER SHRIMP (Penaeus monodon THROUGH PROBIOTICS APPLICATION

    Directory of Open Access Journals (Sweden)

    Irsyaphiani Insan

    2015-12-01

    Full Text Available The study was carried out in Brebes District, the North coast of Java. Tiger shrimp farming in Indonesia, particularly in this area faced some problems which caused by improper pond preparation, disease, and low seed quality. Probiotic was applied in pond to solve this problem. The aim of this study was to evaluate the production of tiger shrimp in ponds with probiotic applications. Six experimental ponds (each measuring 0.5 ha were selected of which three were probiotic ponds and three were controlled. Tiger shrimp postlarvae (PL-30 were stocked at density of four shrimps/m2. Tiger shrimps were reared for three months. Shrimps were fed by commercial pellet. In the first month, shrimp were fed about 7%-5% of the total biomass; in the second months, 3.5%-3% of the total biomass; and in the third month, 2.5%-2% of the total biomass. The treatments in this study were the application of probiotics with concentration of 3 mg/L that were given every five days and control (without probiotics. The results showed the rearing period was 92 ± 6 days in probiotic ponds and 76 ± 16 days in controlled pond. The shrimp in controlled pond should be harvest earlier caused by the high mortality. The average final weight was 16.2 ± 0.7 g in probiotic pond and 15.6 ± 1.9 g in controlled pond. The survival rate was 64.13 ± 12.63% in probiotic pond and 44.17 ± 14.15% in controlled pond. Production was 208 ± 46 kg/pond/cycle in probiotic pond and 123 ± 6 kg/pond/cycle in controlled pond. The result showed that probiotic plays an important role in maintaining water quality parameters and health management as well as increases the survival of shrimp.

  18. Aplicación del bioensayo de Artemia salina en el análisis general de plantas medicinales brasileñas

    OpenAIRE

    Montanher, Ana Beatriz Pimentel; Pizzolatti, Moacir Geraldo; Brighente, Inês Maria Costa

    2002-01-01

    Extractos de once especies de la flora brasileña fueron estudiados para evaluar la actividad citotóxica por el test de Artemia salina. Las plantas seleccionadas fueron: Baccharis pseudotenuifolia, Baccharis ligustrina, Baccharis platypoda, Baccharis coridifolia, Polygala paniculata, Polygala sabulosa, Croton celtidifolius, Cyathea phalerata, Trichilia catigua, Eugenia uniflora y Schinus molle. Los resultados obtenidos para los extractos crudos de B. pseudotenuifolia, B. ligustrina, B. c...

  19. Current status of viral diseases in Indian shrimp aquaculture.

    Science.gov (United States)

    Tandel, G M; John, K Riji; Rosalind George, M; Prince Jeyaseelan, M J

    The intensification of aquaculture has been unique in showing the overwhelming changes in global food production in the last 100 years. Presently, it is playing a vital role in the economies of several countries. Conversely, it is also to be noted that the progression of aquaculture has been the foundation of anthropogenic alteration of a gigantic hierarchy and hence not astonishingly, it resulted in spread and emergence of an increasing group of new unknown diseases. In India, Penaeus monodon, black tiger shrimp was previously the foremost-cultivated shrimp species. Subsequently in 2008, the American white leg shrimp Litopenaeus vannamei has effectively replaced it. The change in dominant species has affected disease concerns in India as well as in world shrimp aquaculture. White spot syndrome virus (WSSV) is the most deleterious for both species. Hepatopancreatic parvovirus (HPV), Monodon baculovirus (MBV) and Infectious hypodermal and hematopoietic necrosis virus (IHHNV) are the other significant infectious agents of P. monodon and L. vannamei. An emerging disease of loose shell syndrome (LSS) was already reported from India during late 1998. A more recent disease of L. vannamei in India is monodon slow growth syndrome (MSGS), a component of which seems to be Laem-Singh virus (LSNV). Thus, most of the information in this review relates to new emerging pathogens that threaten the cultivation shrimp industry in India.

  20. Bioaccumulation of animal adenoviruses in the pink shrimp

    Directory of Open Access Journals (Sweden)

    Roger B. Luz

    2015-09-01

    Full Text Available Adenoviruses are among the most promising viral markers of fecal contamination. They are frequently found in the water, sediment and soil of regions impacted by human activity. Studies of the bioaccumulation of enteric viruses in shrimp are scarce. The cities located in the northern coast of the lake systems in Southern Brazil have high urbanization and intensive farming rates, and poor sewage collection and treatment. One hundred (n = 100 Farfantepenaeus paulensis pink-shrimp specimens and 48 water samples were collected from coastal lagoons between June 2012 and May 2013. Water samples were concentrated and the shrimp, mashed. After DNA extraction, samples were analyzed by real time polymerase chain reaction (qPCR in order to detect and quantify viral genomes. Thirty-five percent of shrimp samples were positive for contamination, predominantly by avian adenoviruses. A total of 91.7% of water samples contained adenoviruses DNA, with the human form being the most frequent. Our results provided evidence of significant bioaccumulation of adenoviruses in shrimp, showing the extent of the impact of fecal pollution on aquatic ecosystems.

  1. Magnetically tunable oil droplet lens of deep-sea shrimp

    Science.gov (United States)

    Iwasaka, M.; Hirota, N.; Oba, Y.

    2018-05-01

    In this study, the tunable properties of a bio-lens from a deep-sea shrimp were investigated for the first time using magnetic fields. The skin of the shrimp exhibited a brilliantly colored reflection of incident white light. The light reflecting parts and the oil droplets in the shrimp's skin were observed in a glass slide sample cell using a digital microscope that operated in the bore of two superconducting magnets (maximum strengths of 5 and 13 T). In the ventral skin of the shrimp, which contained many oil droplets, some comparatively large oil droplets (50 to 150 μm in diameter) were present. A distinct response to magnetic fields was found in these large oil droplets. Further, the application of the magnetic fields to the sample cell caused a change in the size of the oil droplets. The phenomena observed in this work indicate that the oil droplets of deep sea shrimp can act as lenses in which the optical focusing can be modified via the application of external magnetic fields. The results of this study will make it possible to fabricate bio-inspired soft optical devices in future.

  2. Growth and survival of Octopus vulgaris (Cuvier 1797 paralarvae fed on three Artemia-based diets complemented with frozen fish flakes, crushed zooplankton and marine microalgae

    Directory of Open Access Journals (Sweden)

    Lidia Fuentes

    2011-07-01

    Full Text Available During one month, paralarvae of common octopus (Octopus vulgaris were fed 3 different diets: (1 Artemia sp. enriched with Isochrysis galbana (AI complemented with sand eel (Hyperoplus lanceolatus flakes (AH; (2 Artemia sp. enriched with crushed marine zooplankton (AZ; and (3 Artemia sp. cultured with Isochrysis galbana and further enriched with the microalga Nannochloropsis sp. (AN. The highest dry weight (1.6179±0.3861 mg was registered with the AN diet and the best average survival (67.0% with the AZ diet. Considering the highest dry weight obtained, the moderate high survival and the fact that with this diet it was possible to attain the adult stage, the AN diet was the most appropriate. The reasons for the best result in growth observed with AN are discussed as: (1 the combination of docosahexaenoic acid (DHA provided by Isochrysis galbana and the high eicosapentaenoic acid (EPA content present in Nannochloropsis sp.; (2 the fact that the higher protein/lipid ratio of this diet improves the final dry weight of the paralarvae; and (3 the fact that Nannochloropsis sp. could inhibit the harmful microflora growth in the rearing tank. Regarding nutritional aspects, DHA content per se is not the only determinant factor for growth and survival of O. vulgaris paralarvae, but the presence of a high protein/lipid ratio and a high phospholipid content in the diet could possibly explain the better quality and strength of the paralarvae.

  3. Emerging viral diseases of fish and shrimp

    Science.gov (United States)

    Winton, James R.; Walker, Peter J.

    2010-01-01

    The rise of aquaculture has been one of the most profound changes in global food production of the past 100 years. Driven by population growth, rising demand for seafood and a levelling of production from capture fisheries, the practice of farming aquatic animals has expanded rapidly to become a major global industry. Aquaculture is now integral to the economies of many countries. It has provided employment and been a major driver of socio-economic development in poor rural and coastal communities, particularly in Asia, and has relieved pressure on the sustainability of the natural harvest from our rivers, lakes and oceans. However, the rapid growth of aquaculture has also been the source of anthropogenic change on a massive scale. Aquatic animals have been displaced from their natural environment, cultured in high density, exposed to environmental stress, provided artificial or unnatural feeds, and a prolific global trade has developed in both live aquatic animals and their products. At the same time, over-exploitation of fisheries and anthropogenic stress on aquatic ecosystems has placed pressure on wild fish populations. Not surprisingly, the consequence has been the emergence and spread of an increasing array of new diseases. This review examines the rise and characteristics of aquaculture, the major viral pathogens of fish and shrimp and their impacts, and the particular characteristics of disease emergence in an aquatic, rather than terrestrial, context. It also considers the potential for future disease emergence in aquatic animals as aquaculture continues to expand and faces the challenges presented by climate change.

  4. Extreme morphologies of mantis shrimp larvae

    Directory of Open Access Journals (Sweden)

    Carolin Haug

    Full Text Available ABSTRACT Larvae of stomatopods (mantis shrimps are generally categorized into four larval types: antizoea, pseudozoea (both representing early larval stages, alima and erichthus (the latter two representing later larval stages. These categories, however, do not reflect the existing morphological diversity of stomatopod larvae, which is largely unstudied. We describe here four previously unknown larval types with extreme morphologies. All specimens were found in the collections of the Zoological Museum, University of Copenhagen and were collected during the Danish Dana Expedition round the world 1928-30. These new larval types all represent erichthus-type larvae, especially differing in their shield morphologies. The shield morphology ranges from almost spherical to rather disc-like, with sometimes extremely elongated spines, but only a general systematic assignment of the larvae was possible. Further investigations of these larvae are crucial to understand their life habits and ecological impact, especially as stomatopod and other crustacean larvae might have a much more important position in the marine ecosystems than their corresponding adults.

  5. Shrimp cephalothorax meal in laying hen diets

    International Nuclear Information System (INIS)

    Salas-Duran, Catalina; Chacon-Villalobos, Alejandro; Zamora-Sanchez, Laura

    2015-01-01

    The effect of shrimp meal (SM) was measure in commercial laying hen diets. Pleuroncodes planipes was used in Costa Rica, from April to September 2013, to obtain a meal (SM) with a yield of 15%, particle size of 256 μg and negative for Salmonella sp. Proximate analysis was performed to the SM: crude protein (40,67%), ether extract (11,05%), crude fiber (7,12%), ash (27,48%), calcium (9,03%), phosphorus (2,66%), amino acid profile, pepsin digestibility (84%) and acidity (8,34). Subsequently, a trial was performed with 140 40-week-old Hy-Line Brown laying hens, fed with four different diets containing increasing levels of inclusion of SM (0%, 5%, 10%, and 15%) during four weeks; and formulated according to the ideal protein and digestible amino acids concepts; being isocaloric and isoproteic. The variables experimentally evaluated were: production percentage, feed intake, body weight, mortality, egg weight and feed conversion ratio. Only egg weight changed significantly between treatments in the third week (p [es

  6. Fermentation cover brine reformulation for cucumber processing with low salt to reduce bloater defect

    Science.gov (United States)

    Reformulation of calcium chloride cover brine for cucumber fermentation was explored as a mean to minimize the incidence of bloater defect. This study particularly focused on cover brine supplementation with calcium hydroxide, sodium chloride (NaCl), and acids to enhance buffer capacity, inhibit the...

  7. Brine Pockets in the Icy Shell on Europa: Distribution, Chemistry, and Habitability

    Science.gov (United States)

    Zolotov, M. Yu; Shock, E. L.; Barr, A. C.; Pappalardo, R. T.

    2004-01-01

    On Earth, sea ice is rich in brine, salt, and gas inclusions that form through capturing of seawater during ice formation. Cooling of the ice over time leads to sequential freezing of captured sea-water, precipitation of salts, exsolution of gases, and formation of brine channels and pockets. Distribution and composition of brines in sea ice depend on the rate of ice formation, vertical temperature gradient, and the age of the ice. With aging, the abundance of brine pockets decreases through downward migration. De- spite low temperatures and elevated salinities, brines in sea ice provide a habitat for photosynthetic and chemosynthetic organisms. On Europa, brine pockets and channels could exist in the icy shell that may be from a few km to a few tens of km thick and is probably underlain by a water ocean. If the icy shell is relatively thick, convection could develop, affecting the temperature pattern in the ice. To predict the distribution and chemistry of brine pockets in the icy shell we have combined numerical models of the temperature distribution within a convecting shell, a model for oceanic chemistry, and a model for freezing of Europan oceanic water. Possible effects of brine and gas inclusions on ice rheology and tectonics are discussed.

  8. Recovery of biomolecules from marinated herring (Clupea harengus) brine using ultrafiltration through ceramic membranes

    DEFF Research Database (Denmark)

    Gringer, Nina; Hosseini, Seyed Vali; Svendsen, Tore

    2015-01-01

    Marinated herring processing brines, which are usually discarded, are rich in salt, protein, non-protein nitrogen, iron, fatty acids, antioxidant and even possess enzymatic activity. This study investigated the performance of ceramic ultrafiltration of two herring spice brines with a major focus...

  9. Corrosion and hydrogen permeation of A216 Grade WCA steel in hydrothermal magnesium-containing brines

    International Nuclear Information System (INIS)

    Haberman, J.H.; Frydrych, D.J.; Westerman, R.E.

    1988-03-01

    Corrosion rates determined at 1 month in 150/degree/C brine increased with magnesium concentration. The structure of the corrosion product, as determined by x-ray diffraction, depended upon the magnesium concentration. In brines with less than 10,000 ppM magnesium, the primary corrosion product had a spinel structure characteristic of magnetite or magnesioferrite. In brines containing magnesium concentrations greater than 20,000 ppM, the primary corrosion product had the amakinite structure characteristic of a complex iron-magnesium hydroxide. The high corrosion rates observed in brines containing high magnesium concentrations suggest that the corrosion products having the amakinite structure is less protective than corrosion products having the spinel structure. Corrosion rates in high-magnesium (inclusion) brine determined over a 6-month test duration were essentially constant. Hydrogen permeation rates observed in exposing mild steel to high-Mg/sup 2/plus// brine at 150/degree/C could be potentially damaging to a mild steel waste package container. The rate of hydrogen permeation was proportional to the brine flow rate in the autoclave. Thiourea additions to the brine increased the hydrogen permeation rate; sulfate and bromide ion additions did not. The maximum gaseous hydrogen pressure attainable is not known (based on 3Fe /plus/ 4H 2 O /plus/ Fe(sub 3)O /plus/ 4H 2 , would be /approximately/900 atmospheres), and the dependence of permeation rate on temperature is not known. 8 refs., 13 figs., 3 tabs

  10. Geochemistry of Salado Formation brines recovered from the Waste Isolation Pilot Plant (WIPP) repository

    International Nuclear Information System (INIS)

    Abitz, R.; Myers, J.; Drez, P.; Deal, D.

    1990-01-01

    Intergranular brines recovered from the repository horizon of the Waste Isolation Pilot Plant (WIPP) have major- and trace-element compositions that reflect seawater evaporation and diagenetic processes. Brines obtained from repository drill holes are heterogenous with respect to composition, but their compositional fields are distinct from those obtained from fluid inclusions in WIPP halite. The heterogeneity of brine compositions within the drill-hole population indicates a lack of mixing and fluid homogenization within the salt at the repository level. Compositional differences between intergranular (drill hole) and intragranular (fluid inclusions) brines is attributed to isolation of the latter from diagenetic fluids that were produced from dehydration reactions involving gypsum and clay minerals. Modeling of brine-rock equilibria indicates that equilibration with evaporite minerals controls the concentrations of major elements in the brine. Drill-hole brines are in equilibrium with the observed repository minerals halite, anhydrite, magnesite, polyhalite and quartz. The equilibrium model supports the derivation of drill-hole brines from near-field fluid, rather than large-scale vertical migration of fluids from the overlying Rustler or underlying Castile Formations. 13 refs., 6 figs., 6 tabs

  11. Geochemistry of Salado formation brines recovered from the Waste Isolation Pilot Plant (WIPP) repository

    International Nuclear Information System (INIS)

    Abitz, R.; Myers, J.; Drez, P.; Deal, D.

    1990-01-01

    Intergranular brines recovered from the repository horizon of the Waste Isolation Pilot Plant (WIPP) have major- and trace-element compositions that reflect seawater evaporation and diagenetic processes. Brines obtained from repository drill holes are heterogeneous with respect to composition but their compositional fields are distinct from those obtained from fluid inclusions in WIPP halite. The heterogeneity of brine compositions within the drill-hole population indicates a lack of mixing and fluid homogenization within the salt at the repository level. Compositional differences between intergranular (drill hole) and intragranular (fluid inclusions) brines is attributed to isolation of the latter from diagenetic fluids that were produced from dehydration reactions involving gypsum and clay minerals. Modeling of brine-rock equilibria indicates that equilibration with evaporite minerals controls the concentrations of major elements in the brine. Drill-hole brines are in equilibrium with the observed repository minerals halite, anhydrite, magnesite, polyhalite and quartz. The equilibrium model supports the derivation of drill-hole brines from near-field fluid, rather than large-scale vertical migration of fluids from the overlying Rustler or underlying Castile Formations. 13 refs., 6 figs., 6 tabs

  12. Water Recovery from Brine in the Short and Long Term: A KSC Approach

    Science.gov (United States)

    Lunn, Griffin; Melendez, Orlando; Anthony, Steve

    2014-01-01

    KSC has spent many years researching Hollow Fiber Membrane Bioreactors as well as research encompassing:Alternate ammonia removal/Advanced oxidation. Brine purification technologies KSC-ISRU has built an electrolysis cell for the removal of acids in ISRU mining brines. Our goal is to combine all such technologies.

  13. Improving the performance of brine wells at Gulf Coast strategic petroleum reserve sites

    Energy Technology Data Exchange (ETDEWEB)

    Owen, L.B.; Quong, R. (eds.)

    1979-11-05

    At the request of the Department of Energy, field techniques were developed to evaluate and improve the injection of brine into wells at Strategic Petroleum Reserve (SPR) sites. These wells are necessary for the disposal of saturated brine removed from salt domes where oil is being stored. The wells, which were accepting brine at 50 percent or less of their initial design rates, were impaired by saturated brine containing particulates that deposited on the sand face and in the geologic formation next to the wellbore. Corrosion of the brine-disposal pipelines and injection wells contributed to the impairment by adding significant amounts of particulates in the form of corrosion products. When tests were implemented at the SPR sites, it was found that the poor quality of injected brines was the primary cause of impaired injection; that granular-media filtration, when used with chemical pretreatment, is an effective method for removing particulates from hypersaline brine; that satisfactory injection-well performance can be attained with prefiltered brines; and that corrosion rates can be substantially reduced by oxygen-scavenging.

  14. The use of bacconcentrate Herobacterin in brine cheese technology

    Directory of Open Access Journals (Sweden)

    I. Slyvka:

    2017-12-01

    Full Text Available In the article a comparative analysis of the use of the bacterial preparation Herobacterin and the starter RSF-742 (Chr. Hansen, Denmark in the technology of brine cheese was conducted. Herobacterin is a bacterial preparation created using bacteria Lactococcus lactis, Lactobacillus plantarum, Enterococcus faecium, Leuconostoc mesenteroides and Lactococcus garvieae, isolated from traditional Carpathian brine cheese brynza and identified using classical microbiological and modern molecular genetic methods (RAPD-PCR, RFLP-PCR, sequencing of the 16S rRNA gene. The results of investigations of organoleptic, physico-chemical, syneretical and microbiological parameters of cheese brynza with use of preparation Herobacterin are presented in comparison with the starter RSF-742, which includes cultures: Lactococcus lactis subsp. сremoris, Lactococcus lactis subsp. lactis, Streptococcus thermophilus, Lactobacillus helveticus. The use of Herobacterin has a positive effect on organoleptic, physico-chemical and microbiological parameters, all parameters complied with the requirements of DSTU 7065:2009. The level of survival of lactic acid bacteria in brynza during maturation and storage is high, which confirms the correctness of the selection of strains to preparation Herobakterin, which demonstrated good adaptability to the composition and properties of ewe's milk.

  15. Observations of brine plumes below melting Arctic sea ice

    Directory of Open Access Journals (Sweden)

    A. K. Peterson

    2018-02-01

    Full Text Available In sea ice, interconnected pockets and channels of brine are surrounded by fresh ice. Over time, brine is lost by gravity drainage and flushing. The timing of salt release and its interaction with the underlying water can impact subsequent sea ice melt. Turbulence measurements 1 m below melting sea ice north of Svalbard reveal anticorrelated heat and salt fluxes. From the observations, 131 salty plumes descending from the warm sea ice are identified, confirming previous observations from a Svalbard fjord. The plumes are likely triggered by oceanic heat through bottom melt. Calculated over a composite plume, oceanic heat and salt fluxes during the plumes account for 6 and 9 % of the total fluxes, respectively, while only lasting in total 0.5 % of the time. The observed salt flux accumulates to 7.6 kg m−2, indicating nearly full desalination of the ice. Bulk salinity reduction between two nearby ice cores agrees with accumulated salt fluxes to within a factor of 2. The increasing fraction of younger, more saline ice in the Arctic suggests an increase in desalination processes with the transition to the new Arctic.

  16. Observations of brine plumes below melting Arctic sea ice

    Science.gov (United States)

    Peterson, Algot K.

    2018-02-01

    In sea ice, interconnected pockets and channels of brine are surrounded by fresh ice. Over time, brine is lost by gravity drainage and flushing. The timing of salt release and its interaction with the underlying water can impact subsequent sea ice melt. Turbulence measurements 1 m below melting sea ice north of Svalbard reveal anticorrelated heat and salt fluxes. From the observations, 131 salty plumes descending from the warm sea ice are identified, confirming previous observations from a Svalbard fjord. The plumes are likely triggered by oceanic heat through bottom melt. Calculated over a composite plume, oceanic heat and salt fluxes during the plumes account for 6 and 9 % of the total fluxes, respectively, while only lasting in total 0.5 % of the time. The observed salt flux accumulates to 7.6 kg m-2, indicating nearly full desalination of the ice. Bulk salinity reduction between two nearby ice cores agrees with accumulated salt fluxes to within a factor of 2. The increasing fraction of younger, more saline ice in the Arctic suggests an increase in desalination processes with the transition to the new Arctic.

  17. Ecological generalism facilitates the evolution of sociality in snapping shrimps.

    Science.gov (United States)

    Brooks, Katherine C; Maia, Rafael; Duffy, J Emmett; Hultgren, Kristin M; Rubenstein, Dustin R

    2017-12-01

    Evidence from insects and vertebrates suggests that cooperation may have enabled species to expand their niches, becoming ecological generalists and dominating the ecosystems in which they occur. Consistent with this idea, eusocial species of sponge-dwelling Synalpheus shrimps from Belize are ecological generalists with a broader host breadth and higher abundance than non-eusocial species. We evaluate whether sociality promotes ecological generalism (social conquest hypothesis) or whether ecological generalism facilitates the transition to sociality (social transition hypothesis) in 38 Synalpheus shrimp species. We find that sociality evolves primarily from host generalists, and almost exclusively so for transitions to eusociality. Additionally, sponge volume is more important for explaining social transitions towards communal breeding than to eusociality, suggesting that different ecological factors may influence the independent evolutionary origins of sociality in Synalpheus shrimps. Ultimately, our results are consistent with the social transition hypothesis and the idea that ecological generalism facilitates the transition to sociality. © 2017 John Wiley & Sons Ltd/CNRS.

  18. Detection of irradiation in cuticles of commercial shrimps

    International Nuclear Information System (INIS)

    Lehner, K.; Stachowicz, W.

    2006-01-01

    Laboratory for Detection of Irradiated Food has more and more orders for detection of irradiation in shrimps. A method adapted to achieve detection of irradiation in this material is a thermoluminescence measure. However, the procedure is time-consuming - needs several days to receive the result. The aim of presented study was to elaborate the EPR examination of the possible irradiation in this product. As a result od the performed studies it can be concluded, that the EPR measurement of cuticles of shrimps can be adapted as the preliminary, screening test proving the irradiation of shrimp. It has to be stressed, however, that the lack of a specific, hydroxyapatite born EPR signal in the spectrum cannot suggest that sample was not irradiated

  19. Evolution of mineralizing brines in the east Tennessee Mississippi Valley-type ore field

    Energy Technology Data Exchange (ETDEWEB)

    Kesler, S.E.; Gesink, J.A.; Haynes, F.M. (Univ. of Michingan, Ann Arbor (USA))

    1989-05-01

    The east Tennessee Mississippi Valley-type (MVT) ore field contains barite-fluorite and sphalterite deposits in a continuous paleoaquifer consisting of breccia zones in the Upper Cambrian-Lower Ordovician Knox Group. Paragenetic observations and fluid inclusion compositions in these deposits indicate that the Knox paleoaquifer was invaded first by Ca-rich brines (Ca:Na about 1) that deposited fluorite and barite, and later by Na-Ca brines (Ca:Na = 0.1 to 0.5) that deposited sphalerite. Geologic relation sindicate that these brines were derived from the southeast, in the area of the Middle Ordovician Servier foreland shale basin, and that imposed by fluorite solubility indicate further that all original connate water in the Sevier basin was required to deposit the estimated flourite reserves of the ore field.Thus, the later, sphalerite-depositing brines represent recycled meteoric water from the Sevier basin or connate brines from underlying (Cambrian) shales.

  20. Indoor tests to investigate the effect of brine depth on the performance of solar still

    Energy Technology Data Exchange (ETDEWEB)

    Ali, Marwah A.W.; Jabbar N. Khalifa, Abdul [Nahrain University, College of Engineering, Jadiriya, P.O. Box 64040, Baghdad (Iraq)

    2013-07-01

    Many experimental and numerical studies have been done on different configurations of solar stills to optimize the design by examining the effect of climatic, operational and design parameters on its performance. One of the most important of the operational parameters that has received a considerable attention in the literature is the brine depth. This paper reports indoor experimental investigations on the effect of brine depth on the productivity and efficiency of the solar stills at four different brine depths of 1.5, 2, 4 and 5.5 cm. Indoor tests were used by simulating the solar input by proper electric heaters located at the bottom of the still for heating the water contained in the basin of the still. The present study validated the decreasing trend in productivity with the increase of brine depth and showed that the still productivity could be influenced by the brine depth by up to 24%.

  1. PERFORMANCES OF TIGER SHRIMP CULTURE IN ENVIRONMENTALLY FRIENDLY PONDS

    Directory of Open Access Journals (Sweden)

    Taufik Ahmad

    2016-10-01

    Full Text Available Mangrove ecosystem plays an obvious role in maintaining the biological balance in the coastal environment where shrimp ponds are usually constructed. The removal of mangroves around shrimp ponds has frequently brought about harvest failure. The study evaluated the performance of tiger shrimp culture in ponds provided with water from a water body where there was mangrove vegetation (hereafter mangrove reservoir. Twelve ponds, each measuring 2,500 m2, were filled with seawater from the mangrove reservoir until the water depth of 100 cm and then stocked with 20-40 PL/m2. In the first six ponds, the bottom water was released into the reservoir when the water depth reached 140 cm and then the water depth was maintained at 100 cm. In the second six ponds, the water was released from the ponds until the water depth reached 60 cm and then refilled with reservoir water until a depth of 100 cm. Both treatment ponds received water from the reservoir which also received the wastewater. The feeds for the shrimps were broadcast into the ponds twice a day to meet the 3% shrimp biomass requirement, which adjusted every other week through sampling. The result showed that mangrove  vegetation is capable of removing excessive nutrients, up to 70% for NO3- N and NH4 +-N, reducing PO4 =-P fluctuation, and producing bioactive  compounds. In the second treatment ponds, shrimp mortality started to occur in day 28 and most died by day 54 after stocking due to white spot disease outbreak. Mass mortality took place 54 days after stocking in two out of six of the first treatment ponds.

  2. The Effect of Latitudinal Variation on Shrimp Reproductive Strategies.

    Science.gov (United States)

    van de Kerk, Madelon; Jones Littles, Chanda; Saucedo, Omar; Lorenzen, Kai

    2016-01-01

    Reproductive strategies comprise the timing and frequency of reproductive events and the number of offspring per reproductive event, depending on factors such as climate conditions. Therefore, species that exhibit plasticity in the allocation of reproductive effort can alter their behavior in response to climate change. Studying how the reproductive strategy of species varies along the latitudinal gradient can help us understand and predict how they will respond to climate change. We investigated the effects of the temporal allocation of reproductive effort on the population size of brown shrimp (Farfantepenaeus aztecus) along a latitudinal gradient. Multiple shrimp species exhibit variation in their reproductive strategies, and given the economic importance of brown shrimp to the commercial fishing sector of the Unites States, changes in the timing of their reproduction could have significant economic and social consequences. We used a stage-based, density-dependent matrix population model tailored to the life history of brown shrimp. Shrimp growth rates and environmental carrying capacity were varied based on the seasonal climate conditions at different latitudes, and we estimated the population size at equilibrium. The length of the growing season increased with decreasing latitude and the reproductive strategy leading to the highest population size changed from one annual birth pulse with high reproductive output to continuous low-output reproduction. Hence, our model confirms the classical paradigm of continuous reproduction at low latitudes, with increased seasonality of the breeding period towards the poles. Our results also demonstrate the potential for variation in climate to affect the optimal reproductive strategy for achieving maximum population sizes. Certainly, understanding these dynamics may inform more comprehensive management strategies for commercially important species like brown shrimp.

  3. The Effect of Latitudinal Variation on Shrimp Reproductive Strategies.

    Directory of Open Access Journals (Sweden)

    Madelon van de Kerk

    Full Text Available Reproductive strategies comprise the timing and frequency of reproductive events and the number of offspring per reproductive event, depending on factors such as climate conditions. Therefore, species that exhibit plasticity in the allocation of reproductive effort can alter their behavior in response to climate change. Studying how the reproductive strategy of species varies along the latitudinal gradient can help us understand and predict how they will respond to climate change. We investigated the effects of the temporal allocation of reproductive effort on the population size of brown shrimp (Farfantepenaeus aztecus along a latitudinal gradient. Multiple shrimp species exhibit variation in their reproductive strategies, and given the economic importance of brown shrimp to the commercial fishing sector of the Unites States, changes in the timing of their reproduction could have significant economic and social consequences. We used a stage-based, density-dependent matrix population model tailored to the life history of brown shrimp. Shrimp growth rates and environmental carrying capacity were varied based on the seasonal climate conditions at different latitudes, and we estimated the population size at equilibrium. The length of the growing season increased with decreasing latitude and the reproductive strategy leading to the highest population size changed from one annual birth pulse with high reproductive output to continuous low-output reproduction. Hence, our model confirms the classical paradigm of continuous reproduction at low latitudes, with increased seasonality of the breeding period towards the poles. Our results also demonstrate the potential for variation in climate to affect the optimal reproductive strategy for achieving maximum population sizes. Certainly, understanding these dynamics may inform more comprehensive management strategies for commercially important species like brown shrimp.

  4. Ozone Disinfection of Vibrio vulnificus in Shrimp Pond Water

    Science.gov (United States)

    Dyah Pita Rengga, Wara; Cahya Julyta Putri, Echa; Wulansarie, Ria; Suryanto, Agus

    2018-03-01

    One variety of shrimp, L.Vanamei, often uses brackish water during the operation in the shrimp pond. Chlorination and ultraviolet are usually used for disinfection of brackish water. However, it is ineffective and forms sediment in the water distribution. It can be a negative impact on the water quality cause a contamination on the shrimp, so the farmers might have loss of profit because Vibrio vulnificus causes infection and dead on the shrimp. It affects the safety of consumers and should be minimized. The purpose of this study is to reduce the number of V. vulnificus bacteria in the pond water. The water was put in the storage tanks then pumped to filter out the impurities of the water. Furthermore, the water set the flow rate in 1 LPM, 2 LPM, and 3 LPM. After that, the ozone was injected to the water flow to sterilize the V. vulnificus bacteria. Finally, the water was returned to the original tank. The water from the tank was taken through a valve and analyzed in 0, 3, 7, 12, 18, 24, 30 minutes. The sample was analyzed immediately using a Total Plate Count method to determine the number of V. vulnificus bacteria in the shrimp pond water. The flow rate shows that the longer time of ozone made a lower amount of Vibrio v. bacteria. In 2 LPM water, it shows the optimum results of V. vulnificus. bacteria reduction for 88.1% compared to the flow rate of 1 LPM and 3 LPM with the bacteria reduction of 68,8% and 70.6%. This study shows that the ozone with a flow rate of 2 LPM circulation is the most effective method to help reducing the number of V. vulnificus in brackish water distribution system in the shrimp environment and potentially as a disinfectant.

  5. Oil exudation and histological structures of duck egg yolks during brining.

    Science.gov (United States)

    Lai, K M; Chung, W H; Jao, C L; Hsu, K C

    2010-04-01

    Changes in oil exudation and histological structures of salted duck egg yolks during brining up to 5 wk were investigated. During brining, the salt contents of albumen, exterior yolk (hardened portion), and interior yolk (soft or liquid portion) gradually increased accompanied by slight decreases in moisture content. The hardening ratio of salted egg yolks increased rapidly to about 60% during the first week of brining and then reached 100% at the end of brining. After brining, part of the lipids in salted egg yolk became free due to the structural changes of low-density lipoprotein induced by dehydration and increase of salt content, and more free lipids in salted egg yolk were released after the cooking process. With the brining time increased up to 5 wk, the outer region of the cooked salted yolk gradually changed into dark brown, brown, orange, and then dark brown, whereas the center region changed into light yellow, yellow, dark yellow, and then yellow again. The microstructures of cooked salted egg yolks showed that the yolk spheres in the outer and middle regions retained their original shape, with some shrinking and being packed more loosely when brining time increased, and the exuded oil filled the space between the spheres. Furthermore, the yolk spheres in the center region transformed to a round shape but still showed granulation after 4 wk of brining, whereas they were mostly disrupted after 2 to 5 wk of brining. One of the most important characteristics of cooked salted egg yolks, gritty texture, contributed to oil exudation and granulated yolk spheres were observed at the brining time of 4 wk.

  6. Effect of irradiation on biochemistry properties of shrimp allergen

    International Nuclear Information System (INIS)

    Gu Kefei; Gao Meixu; Li Chunhong; Li Shurong; Pan Jiarong

    2007-01-01

    Study on the effects of 60 Co γ-rays irradiation at the dose of 0,3,5,7,10 kGy on shrimp allergen biochemistry properties was conducted. The results indicated that the allergen protein molecule can be broken down to smaller molecules or coagulated to larger molecules by irradiation. The hydrophobicity and turbidity of irradiated allergen increased with the increase of absorbed dose. The results also show that allergen solution is more sensitive to irradiation than allergen in solid state or in the whole shrimp. (authors)

  7. Analysis of the sulphite content in shrimps and prawns

    Directory of Open Access Journals (Sweden)

    Elisabetta Bonerba

    2013-06-01

    Full Text Available Food additives are redefined in European legislation (EC Regulation No. 1333/2008. Sulphur dioxide (E220 and sulphite (E221- E228 are widely used in food processing as preservatives because they slow down bacterial growth on foods and prevent oxidation or browning developing on shrimp and lobster. Shellfish processors, farmers and fishermen have long used sulfiting agents in a variety of species of warm and coldwater crustaceans as a treatment to prevent prawns and shrimps melanosis (blackspot, which is a natural process that makes the shell black after harvesting caused by Polyphenoloxidase enzyme systems which remain active during refrigeration or ice storage. Sulfite-induced hypersensitivity is the most well-established adverse response in humans to this food additive. In the present study the presence of sulfites in different frozen and thawed shrimp and prawn species belonging to Penaeoidea superfamily has been evaluated by the Monier-Williams procedure, in order to carry out a risk assessment and evaluate the levels of consumer exposure to this class of additives from these fish products. In addition to assessing and monitoring the correct use of the additive, according to the limits imposed by the European regulations, the correct consumer information on labels was also evaluated. Analysis were performed on both whole shrimp (shell on and inedible parts (head and peeled shell. Sulphites concentration in frozen samples (expressed as SO2 mg/kg mean value±S.D. was 214±17.43 for head on shell on shrimps; 170.73±14.99 for shell on headless shrimps; 112.90±27.55 for peeled and deveined shrimps. Thawed shrimps were purchased at mass retailers channel and local fish markets and local seafood retailers and purveyors: for these samples, all head on shell on, the sulphites concentration (expressed as SO2 mg/kg mean value±S.D. was 160.05±26.15 and 292.54±146.04, respectively. Non-edible parts showed, in all samples, much higher concentrations.

  8. SHRIMP MARICULTURE DEVELOPMENT IN ECUADOR: SOME RESOURCE POLICY ISSUES

    OpenAIRE

    Southgate, Douglas

    1992-01-01

    During the past 15 years, Ecuador has become the Western Hemisphere's leading producer and exporter of shrimp. Growth has come about largely through mariculture development. About 8,000 metric tons (MT) of shrimp have been captured off the Ecuadorian coast each year since the late 1970s. Meanwhile, pond output has increased several-fold, from less than 5,000 MT in 1979 to over 100,000 MT 12 years later (Table 1). Mariculture has expanded largely at the expense of renewable natural resources. ...

  9. A quantitative method to measure and evaluate the peelability of shrimps (Pandalus borealis)

    DEFF Research Database (Denmark)

    Gringer, Nina; Dang, Tem Thi; Orlien, Vibeke

    2018-01-01

    A novel, standardized method has been developed in order to provide a quantitative description of shrimp peelability. The peeling process was based on the measure of the strength of the shell-muscle attachment of the shrimp using a texture analyzer, and calculated into the peeling work. The self......-consistent method, insensitive of the shrimp size, was proven valid for assessment of ice maturation of shrimps. The quantitative peeling efficiency (peeling work) and performance (degree of shell removal) showed that the decrease in peeling work correlated with the amount of satisfactory peeled shrimps, indicating...... an effective weakening of the shell-muscle attachment. The developed method provides the industry with a quantitative analysis for measurement of peeling efficiency and peeling performance of shrimps. It may be used for comparing different maturation conditions in relation to optimization of shrimps peeling....

  10. The effects of a thermophile metabolite, tryptophol, upon protecting shrimp against white spot syndrome virus.

    Science.gov (United States)

    Zhu, Fei; Jin, Min

    2015-12-01

    White spot syndrome virus (WSSV) is a shrimp pathogen responsible for significant economic loss in commercial shrimp farms and until now, there has been no effective approach to control this disease. In this study, tryptophol (indole-3-ethanol) was identified as a metabolite involved in bacteriophage-thermophile interactions. The dietary addition of tryptophol reduced the mortality in shrimp Marsupenaeus japonicus when orally challenged with WSSV. Our results revealed that 50 mg/kg tryptophol has a better protective effect in shrimp than 10 or 100 mg/kg tryptophol. WSSV copies in shrimp were reduced significantly (P shrimp, and thus holds significant promise as a novel and efficient therapeutic approach to control WSSV in shrimp aquaculture. Copyright © 2015 Elsevier Ltd. All rights reserved.

  11. Topography and stoichiometry of acidic proteins in large ribosomal subunits from Artemia salina as determined by crosslinking

    International Nuclear Information System (INIS)

    Uchiumi, T.; Wahba, A.J.; Traut, R.R.

    1987-01-01

    The 60S subunits isolated from Artemia salina ribosomes were treated with the crosslinking reagent 2-iminothiolane under mild conditions. Proteins were extracted and fractions containing crosslinked acidic proteins were obtained by stepwise elution from CM-cellulose. Each fraction was analyzed by diagonal (two-dimensional nonreducing-reducing) NaDodSO 4 /polyacrylamide gel electrophoresis. Crosslinked proteins below the diagonal were radioiodinated and identified by two-dimensional acidic urea-NaDodSO 4 gel electrophoresis. Each of the acidic proteins P1 and P2 was crosslinked individually to the same third protein, PO. The fractions containing acidic proteins were also analyzed by two-dimensional nonequilibrium isoelectric focusing-NaDodSO 4 /polyacrylamide gel electrophoresis. Two crosslinked complexes were observed that coincide in isoelectric positions with monomeric P1 and P2, respectively. Both P1 and P2 appear to form crosslinked homodimers. These results suggest the presence in the 60S subunit of (P1) 2 and (P2) 2 dimers, each of which is anchored to PO. Protein PO appears to play the same role as L10 in Escherichia coli ribosomes and may form a pentameric complex with the two dimers in the 60S subunits

  12. Efficiency of Artemia cysts removal as a model invasive spore using a continuous microwave system with heat recovery.

    Science.gov (United States)

    Balasubramanian, Sundar; Ortego, Jeffrey; Rusch, Kelly A; Boldor, Dorin

    2008-12-15

    A continuous microwave system to treat ballast water inoculated with Artemia salina cysts as a model invasive spore was tested for its efficacy in inactivating the cysts present. The system was tested at two different flow rates (1 and 2 L x min(-1)) and two different power levels (2.5 and 4.5 kW). Temperature profiles indicate that the system could deliver heating loads in excess of 100 degrees C in a uniform and near-instantaneous manner when using a heat recovery system. Except for a power and flow rate combination of 2.5 kW and 2 L x min(-1), complete inactivation of the cysts was observed at all combinations at holding times below 100 s. The microwave treatment was better or equal to the control treatment in inactivating the cysts. Use of heat exchangers increased the power conversion efficiency and the overall efficiency of the treatment system. Cost economics analysis indicates that in the present form of development microwave treatment costs are higher than the existing ballast water treatment methods. Overall, tests results indicated that microwave treatment of ballast water is a promising method that can be used in conjunction with other methods to form an efficient treatment system that can prevent introduction of potentially invasive spore forming species in non-native waters.

  13. Plâncton, Artemia sp, dieta artificial e suas combinações no desenvolvimento e sobrevivência do quinguio (Carassius auratus durante a larvicultura Plankton, Artemia sp, artificial diet and their combinations on the development and survival of goldfish (Carassius auratus during larviculture phase

    Directory of Open Access Journals (Sweden)

    Eliana Maria Galdioli

    2000-05-01

    Full Text Available O objetivo deste estudo foi verificar a influência do uso de diferentes alimentos sobre o desenvolvimento de larvas de quinguio (Carassius auratus, assim como determinar o melhor manejo alimentar. O delineamento experimental utilizado foi o inteiramente casualizado com cinco tratamentos e quatro repetições. Para análise estatística utilizou-se o teste de Tukey. Utilizaram-se 20 aquários com capacidade para 10 L, sendo estocadas 20 larvas com 5,20 mm de comprimento por aquário. Os tratamentos utilizados foram: somente ração com 56,00% de proteína bruta (R; plâncton (PL; náuplios de Artemia sp (NA, ração + plâncton (R+PL e ração + náuplios de Artemia sp (R+NA, sendo seu fornecimento à vontade duas vezes ao dia. Houve mortalidade de todos os indivíduos submetidos ao tratamento R. O uso de NA e R+NA proporcionou taxas de sobrevivência superiores (p 0,05. Conclui-se que as diferentes fontes de alimento interferem no desempenho das larvas, sendo o manejo alimentar com o fornecimento de ração + náuplios de Artemia sp o mais adequado para a larvicultura do quinguioThe aim of this study was to verify the influence of different diets on the development of goldfish (Carassius auratus larvae and determine the best diet management during its larviculture phase. The experimental design was totally randomized with five treatments and four replicatess, using Tukey test for statistical analysis. Twenty 10L-aquariums were used, where twenty larvae with 5.20 mm length were stocked per aquarium. Quinguio larvae were fed daily ration of different diets: ration alone including 56.00% crude protein (R, plankton (PL, Artemia sp nauplii (AN, ration + plankton (R+PL and ration + Artemia sp nauplii (R+AN, ad libitum twice a day. Treatment R caused the death of all individuals. AN and R+AN diets provided a higher survival rate (p 0.05 was observed between the different treatments. Under the conditions of the present experiment, the results led to

  14. Distribution of metals during digestion by cutthroat trout fed benthic invertebrates contaminated in the Clark Fork River, Montana and the Coeur d'Alene River, Idaho, U.S.A., and fed artificially contaminated Artemia

    Science.gov (United States)

    Farag, A.M.; Suedkamp, M.J.; Meyer, J.S.; Barrows, R.; Woodward, D.F.

    2000-01-01

    The concentrations of essential amino acids in three, undigested invertebrate diets collected from the Clark Fork River (CFR) for cutthroat trout were similar to each other, but were c. 25–75% less than Artemia that were exposed to a mixture of arsenic, copper, cadmium, lead and zinc in the laboratory. The Artemia diet appeared less palatable and the texture, quantity and appearance of the intestinal contents differed between fish fed the Artemia and CFR diets. The Pb% in the fluid fraction of the intestinal contents was greater for the Artemia (29%) than for the CFR diets (10–17%), and the Cu% in the amino acid plus metal fraction of the intestinal contents was greater for the Artemia (78%) than for two of the three CFR diets (67% and 70%). Intestinal contents of fish fed invertebrate diets collected from various sites on the Coeur d'Alene River (CDA), Idaho, were similar in texture, quantity, and appearance. For fish fed the CDA diets, differences in the distribution of metals among fractions of the digestive fluids appeared to be related to concentrations of metals in the invertebrate diets. Pb% was lowest of all metals in the fluid portion of the intestinal contents. However, >80% of all metals in the hind gut were associated with the particulate fraction where they may still be available for uptake through pinocytosis.

  15. Quorum Quenching Bacillus sonorensis Isolated from Soya Sauce Fermentation Brine

    Directory of Open Access Journals (Sweden)

    Kok-Gan Chan

    2012-03-01

    Full Text Available An N-acylhomoserine lactone (AHL-degrading bacterial strain, L62, was isolated from a sample of fermentation brine of Chinese soya sauce by using rich medium agar supplemented with soya sauce (10% v/v. L62, a rod-shaped Gram positive bacterium with amylolytic activity, was phylogentically related to Bacillus sonorensis by 16S ribosomal DNA and rpoB sequence analyses. B. sonorensis L62 efficiently degraded N-3-oxohexanoyl homoserine lactone and N-octanoylhomoserine lactone. However, the aiiA homologue, encoding an autoinducer inactivation enzyme catalyzing the degradation of AHLs, was not detected in L62, suggesting the presence of a different AHL-degrading gene in L62. To the best of our knowledge, this is the first report of AHL-degrading B. sonorensis from soya sauce liquid state fermentation.

  16. Ice Control with Brine Spread with Nozzles on Highways

    DEFF Research Database (Denmark)

    Bolet, Lars; Fonnesbech, Jens Kristian

    2010-01-01

    on the major roads (150 km) in the municipality of North Funen from the winter 2007/8. The result has been a dramatically reduction in the number of traffic accidents on slippery roads during the winter season. From 7 and 5 accidents in the previous 2 winters to 1 accident in the winter 2007/8. Neighbouring...... municipalities had an increasing number of traffic accidents on slippery roads in the same period.......During the years 1996-2006, the former county of Funen, Denmark, gradually replaced pre-wetted salt with brine spread with nozzles as anti-icing agent in all her ice control activities. The replacement related to 1000 kilometres of highways. Jeopardizing neither road safety nor traffic flow...

  17. In-situ fracture mapping using geotomography and brine tracers

    International Nuclear Information System (INIS)

    Deadrick, F.J.; Ramirez, A.L.; Lytle, R.J.

    1981-01-01

    The Lawrence Livermore National Laboratory is currently assessing the capabilities of high resolution geophysical methods to characterize geologic sites for the disposal of high level nuclear waste. A successful experiment has recently been performed in which salt water tracers and high frequency electromagnetic waves were utilized to map rock mass fracture zones in-situ. Multiple cross-borehole EM transmissions were used to generate a tomographic image of the fractured rock region between two boreholes. The tomographs obtained correlate well with conventional wireline geophysical logs which can be used to infer the location of fractured zones in the rock mass. This indirect data suggests that the geotomography and brine tracer technique may have merit in mapping fractured zones between boreholes

  18. In vivo and in vitro antiplasmodial activities of some plants traditionally used in Guatemala against malaria.

    Science.gov (United States)

    Franssen, F F; Smeijsters, L J; Berger, I; Medinilla Aldana, B E

    1997-07-01

    We present an evaluation of the antiplasmodial and cytotoxic effects of four plants commonly used in Guatemalan folk medicine against malaria. Methanol extracts of Simarouba glauca D. C., Sansevieria guineensis Willd, Croton guatemalensis Lotsy, and Neurolaena lobata (L.)R.Br. significantly reduced parasitemias in Plasmodium berghei-infected mice. Dichloromethane fractions were screened for their cytotoxicities on Artemia salina (brine shrimp) larvae, and 50% inhibitory concentrations were determined for Plasmodium falciparum in in vitro cultures. Both chloroquine-susceptible and -resistant strains of P. falciparum were significantly inhibited by these extracts. Of all dichloromethane extracts, only the S. glauca cortex extract was considered to be toxic to nauplii of A. salina in the brine shrimp test.

  19. Dengue antiviral activity of polar extract from Melochia umbellata (Houtt) Stapf var. Visenia

    Science.gov (United States)

    Hariani Soekamto, Nunuk; Liong, S.; Fauziah, S.; Wahid, I.; Firdaus; Taba, P.; Ahmad, F.

    2018-03-01

    A research on the dengue antiviral activity test on the polar bark extract of M. umbelatta (Houtt.) Stapf var. Vicenia have been done to determine the relation to its activity againts brine shrimp Artemia salina. The bark was extracted by maceration with n-hexane, chloroform, and ethylacetate. The activity of the ethyl acetate extract was then tested against A. salina and dengue virus. It was found that the ethyl acetate extract was active to A. salina with the LC50 value of 101.66 μg/mL and also very active to dengue virus with the IC50 value of 1.67μg/mL. It is clear that the toxicity to brine shrimp A. salina has a positive correlation with the dengue anti virus.

  20. CO2/ brine substitution experiments at simulated reservoir conditions

    Science.gov (United States)

    Kummerow, Juliane; Spangenberg, Erik

    2015-04-01

    Capillary properties of rocks affect the mobility of fluids in a reservoir. Therefore, the understanding of the capillary pressure behaviour is essential to assess the long-term behaviour of CO2 reservoirs. Beyond this, a calibration of the petrophysical properties on water saturation of reservoir rocks at simulated in situ conditions is crucial for a proper interpretation of field monitoring data. We present a set-up, which allows for the combined measurements of capillary pressure, electric resistivity, and elastic wave velocities under controlled reservoir conditions (pconf = 400 bar, ppore = 180 bar, T = 65 ° C) at different brine-CO2 saturations. The capillary properties of the samples are measured using the micropore membrane technique. The sample is jacketed with a Viton tube (thickness = 4 mm) and placed between two current electrode endcaps, which as well contain pore fluid ports and ultrasonic P and S wave transducers. Between the sample and the lower endcap the hydrophilic semi-permeable micro-pore membrane (pore size = 100 nm) is integrated. It is embedded into filter papers to establish a good capillary contact and to protect the highly sensitive membrane against mechanical damage under load. Two high-precision syringe pumps are used to displace a quantified volume of brine by CO2 and determine the corresponding sample saturation. The fluid displacement induces a pressure gradient along the sample, which corresponds to the capillary pressure at a particular sample saturation. It is measured with a differential pressure sensor in the range between 0 - 0.2 MPa. Drainage and imbibition cycles are performed to provide information on the efficiency of capillary trapping and to get a calibration of the petrophysical parameters of the sample.

  1. Viruses-to-mobile genetic elements skew in the deep Atlantis II brine pool sediments

    KAUST Repository

    Adel, Mustafa

    2016-09-06

    The central rift of the Red Sea has 25 brine pools with different physical and geochemical characteristics. Atlantis II (ATIID), Discovery Deeps (DD) and Chain Deep (CD) are characterized by high salinity, temperature and metal content. Several studies reported microbial communities in these brine pools, but few studies addressed the brine pool sediments. Therefore, sediment cores were collected from ATIID, DD, CD brine pools and an adjacent brine-influenced site. Sixteen different lithologic sediment sections were subjected to shotgun DNA pyrosequencing to generate 1.47 billion base pairs (1.47 × 109 bp). We generated sediment-specific reads and attempted to annotate all reads. We report the phylogenetic and biochemical uniqueness of the deepest ATIID sulfur-rich brine pool sediments. In contrary to all other sediment sections, bacteria dominate the deepest ATIID sulfur-rich brine pool sediments. This decrease in virus-to-bacteria ratio in selected sections and depth coincided with an overrepresentation of mobile genetic elements. Skewing in the composition of viruses-to-mobile genetic elements may uniquely contribute to the distinct microbial consortium in sediments in proximity to hydrothermally active vents of the Red Sea and possibly in their surroundings, through differential horizontal gene transfer.

  2. Distribution of Cathepsin D Activity between Lysosomes and a Soluble Fraction of Marinating Brine.

    Science.gov (United States)

    Szymczak, Mariusz

    2016-08-01

    This paper is the first ever to describe the phenomenon of bimodal distribution of cathepsin D in the lysosomal and soluble fractions of brine left after herring marinating. Up to 2 times higher cathepsin D activity was observed in the lysosome fraction. Activity of cathepsin D in brine increased according to the logarithmic function during low frequency-high power ultrasounds treatment or according to the linear function after multiple freezing-thawing of brine. Activity enhancement was achieved only in the brine devoid of lipids and suspension. Study results show also that measurement of lysosomal cathepsin D activity in the marinating brine requires also determining cathepsin E activity. Decreasing pore size of microfilter from 2.7 to 0.3 μm significantly reduced the lysosome content in the brine. The presence of lysosomes and the possibility of their separation as well as the likely release of cathepsins shall be considered during industrial application of the marinating brine, as new cathepsins preparations in fish and meat technology. © 2016 Institute of Food Technologists®

  3. Alcohol Brine Freezing of Japanese Horse Mackerel (Trachurus japonicus) for Raw Consumption

    Science.gov (United States)

    Maeda, Toshimichi; Yuki, Atsuhiko; Sakurai, Hiroshi; Watanabe, Koichiro; Itoh, Nobuo; Inui, Etsuro; Seike, Kazunori; Mizukami, Yoichi; Fukuda, Yutaka; Harada, Kazuki

    In order to test the possible application of alcohol brine freezing to Japanese horse mackerel (Trachurus japonicus) for raw consumption, the quality and taste of fish frozen by direct immersion in 60% ethanol brine at -20, -25 and -30°C was compared with those by air freezing and fresh fish without freezing. Cracks were not found during the freezing. Smell of ethanol did not remain. K value, an indicator of freshness, of fish frozen in alcohol brine was less than 8.3%, which was at the same level as those by air freezing and fresh fish. Oxidation of lipid was at the same level as air freezing does, and lower than that of fresh fish. The pH of fish frozen in alcohol brine at -25 and -30°C was 6.5 and 6.6, respectively, which were higher than that by air freezing and that of fresh fish. Fish frozen in alcohol brine was better than that by air and at the same level as fresh fish in total evaluation of sensory tests. These results show that the alcohol brine freezing is superior to air freezing, and fish frozen in alcohol brine can be a material for raw consumption. The methods of thawing in tap water, cold water, refrigerator, and at room temperature were compared. Thawing in tap water is considered to be convenient due to the short thaw time and the quality of thawed fish that was best among the methods.

  4. Migration rates of brine inclusions in single crystals of NaCl

    International Nuclear Information System (INIS)

    Chou, I.M.

    1982-01-01

    Rock-salt deposits have been considered as a possible medium for the permanent storage of high-level radioactive wastes and spent fuel. Brine inclusions present in natural salt can migrate toward the waste if the temperature and the temperature gradients in the vicinity of the radioactive waste are large enough. This migration is due to the dissolution of salt at the hot side of the salt-brine interface, ion diffusion through the brine droplet, and the precipitation of salt at the cold side of the salt brine interface. In order to quantify the problem, the migration rate of these brine inclusions must be estimated under various repository conditions. This paper estimates migration rates for all-liquid brine inclusions in single crystals of NaCl by utilizing recent data for brines and the model of Anthony and Cline [T.R. Anthony and H.E. Cline, J. Appl. Phys., 42, pp. 3380-387 (1971)]. The predictions are compared with experimentally measured migration rates. 4 figures, 6 tables

  5. Uranium mobility in non-oxidizing brines: field and experimental evidence

    International Nuclear Information System (INIS)

    Giblin, A.M.; Appleyard, E.C.

    1987-01-01

    The present distribution of U in the Wollaston Sediments in Saskatchewan can be related to the movement of brines as revealed in Na-Ca-Mg-Cl-metasomes. Experiments were conducted at 60 and 200 0 C under stringently non-oxidizing conditions using solvents ranging from distilled water to a Ca-Na-K brine formulated to simulate the major element composition of the Salton Sea geothermal brines. The experiments were conducted on natural pitchblende (UOsub(2.67)) and synthetic uraninite (UOsub(2.01)). Natural pitchblende was more strongly dissolved than the synthetic uraninite, and the synthetic Salton Sea brine was a more potent solvent than distilled water, 1:4 diluted Salton Sea brine, or pure NaCl brine. Within analytical limits of detection the dissolved U is present in the uranous (U 4+ ) state. The evidence demonstrates empirically the mechanism of dissolution of naturally occurring U minerals in reduced brines and describes a geological case where this appears to have happened. (author)

  6. Juvenile Penaeid Shrimp Density, Spatial Distribution and Size ...

    African Journals Online (AJOL)

    The effects of habitat characteristics (mangrove creek, sandflat, mudflat and seagrass meadow) water salinity, temperature, and depth on the density, spatial distribution and size distribution of juveniles of five commercially important penaied shrimp species (Metapenaus monoceros, M. stebbingi, Fenneropenaeus indicus, ...

  7. Primary carbon sources for juvenile penaeid shrimps in a mangrove ...

    African Journals Online (AJOL)

    Carbon and nitrogen stable isotope ratios were determined in a variety of primary producers (mangroves, epiphytes, phytoplankton and seagrasses), sediments and in five penaeid shrimp species (Penaeus (Fenneropenaeus) indicus, P. japonicus, P. semisulcatus, Metapenaeus monoceros and M. stebbingi), collected ...

  8. Absorption and retention of selenium from shrimps in man

    DEFF Research Database (Denmark)

    Bugel, S. H.; Sandstrom, B.; Larsen, Erik Huusfeldt

    2001-01-01

    This study was undertaken to evaluate the bioavailability of selenium in shrimps, a possible good source of selenium, by measurements of the absorption and retention of selenium and the effects on plasma selenium concentration and glutathione peroxidase activity. Twelve healthy young subjects (9F...... of the study, after 2, 4, and 6 weeks. The selenium intake increased from 39.4 +/- 15.3 mug/d to 127 +/- 5.5 mug/d with the addition of shrimps. The apparent absorption of selenium from shrimps was 83 +/- 4%, Faecal and urinary selenium excretion was 32.5 +/- 17.0 mug/d and 21.2 +/- 9.0 mug/d, re spectively...... and the total retention of selenium was 3.1 +/- 1.1 mg. Plasma selenium concentrations were 95.2 +/- 9.7 mug/L and 101.5 +/- 9.7 mug/L before and after six weeks of shrimp intake, respectively (p...

  9. Prevalence of Staphylococcus aureus in Shrimps in Tehran during 2013

    Directory of Open Access Journals (Sweden)

    Mohammad Mehdi Soltan Dallal

    2015-12-01

    Full Text Available Background During fishing and transport, preservation and quality of fish products are importantas well as storage to prevent the growth of pathogenic and toxin producing bacteria.Staphylococcus aureus is one of the most common causes of sea food-borne diseases worldwidedue to contamination of food by preformed enterotoxins. The aim of this study was to compare theprevalence and contamination of S. aureus in marine and farmed shrimps in Tehran fishery center.Methods: A total of 300 samples, including 150 marine, 150 farmed shrimps were selected duringSeptember 2013 to December 2013. Isolation and identification of S. aureus from isolated sampleswere carried out according to conventional methods, and antibiotic susceptibility test wasperformed by modified Kirby-Bauer disc diffusion methodResults: The results of this study showed that 30% of marine and 20% off armed shrimps werecontaminated with S. aureus. The highest resistance was observed with penicillin and ampicillin,whereas 100% were sensitive to vancomycin, clindamycin, ciprofloxacin, and rifampin.Conclusions: Due to relatively high contamination of shrimp by S. aureus more attention shouldbe given during processing and manufacturing.

  10. Prevalence of Staphylococcus aureus in Shrimps in Tehran during 2013

    Directory of Open Access Journals (Sweden)

    Mohammad Mehdi Soltan Dallal

    2016-02-01

    Full Text Available Background During fishing and transport, preservation and quality of fish products are importantas well as storage to prevent the growth of pathogenic and toxin producing bacteria.Staphylococcus aureus is one of the most common causes of sea food-borne diseases worldwidedue to contamination of food by preformed enterotoxins. The aim of this study was to compare theprevalence and contamination of S. aureus in marine and farmed shrimps in Tehran fishery center.Methods: A total of 300 samples, including 150 marine, 150 farmed shrimps were selected duringSeptember 2013 to December 2014. Isolation and identification of S. aureus from isolated sampleswere carried out according to conventional methods, and antibiotic susceptibility test wasperformed by modified Kirby-Bauer disc diffusion method.Results: The results of this study showed that 30% of marine and 20% off armed shrimps werecontaminated with S. aureus. The highest resistance was observed with penicillin and ampicillin,whereas 100% were sensitive to vancomycin, clindamycin, ciprofloxacin, and rifampin.Conclusions: Due to relatively high contamination of shrimp by S. aureus more attention shouldbe given during processing and manufacturing.

  11. A new alpheid shrimp (Crustacea: Decapoda: Alpheidae) from South Vietnam

    NARCIS (Netherlands)

    Nguyên Van Xuân,

    2001-01-01

    A new alpheid shrimp, Thuylamea camelus gen. nov. & spec. nov., from Dong Hoa, a coastal community of Can Gio district, and northwest of Vung Tau (Cap St. Jacques), Vietnam, is described and illustrated. The new genus appears not to be closely related to any of the previously described genera. It

  12. Lunar Cycles, Catchability of Penaeid Shrimps and Implications for ...

    African Journals Online (AJOL)

    Keywords: Penaeidae, fishing effort, lunar phases, profitability, spatial closures. ... closures during periods of the lunar cycle with predictably low catch-per- ... each lunar phase and month using two-way ANOVA. ... shrimps, for which the CPUE declined throughout the fishing season ... (Garcia, 1988) and abundance of.

  13. Toward Improved Environmental and Social Management of Indian Shrimp Farming

    NARCIS (Netherlands)

    Hein, L.G.

    2002-01-01

    In the last decade, Indian shrimp aquaculture production tripled from 30,000 tons (1990) to 102,000 tons (1999). This fast development, combined with a lack of adequate planning and regulation, caused a number of environmental problems and social conflicts, including conversion of mangroves, water

  14. Genetic diversity of intensive cultured and wild tiger shrimp Penaeus ...

    African Journals Online (AJOL)

    The objective of this study was to assess the genetic diversity of intensive cultured and wild tiger shrimp Penaeus monodon (Fabricius) in Malaysia using six microsatellite markers (CSCUPmo1, CSCUPmo2, CSCUPmo3, CSCUPmo4, CSCUPmo6 and CSCUPmo7). The mean numbers of allele, observed heterozygosis, ...

  15. Population structure and expansion of kuruma shrimp ( Penaeus ...

    African Journals Online (AJOL)

    Sequence analyses on the specific intron from the elongation factor-1α gene were conducted to examine the population genetic structure and expansion of kuruma shrimp (Penaeus japonicus) off Taiwan. Five populations including 119 individuals were separately sampled from the north of East China Sea (ECS), west of ...

  16. Distribution and biology of Indo-Pacific insular hypogeal shrimps

    Science.gov (United States)

    Maciolek, J.A.

    1983-01-01

    Ten species of caridean shrimps, representing nine genera in five families, have been found in exposures of the marine water table at 28 islands from Hawaii to the western Indian Ocean. Synthesis of literature information and personal observations indicate that, as a group, these shrimps are characterized by red body pigment, reduced but pigmented eyes, euryhalinity, a proclivity for interstitial seawater in limestone or lava rock, generalized food requirements, and probable pre-Pleistocene origins. The shrimps have not been found in waters cooler than about 20°C.Species are often solitary, but as many as five are known to coexist. Six of the species have widely scattered populations, some as far apart as Hawaii and the Red Sea. Passive oceanic dispersal is endorsed as a general explanation for such apparently disjunct distributions. On the basis of an assumed primary habitat requirement of interstitial marine water, which could include that in shallow submerged rock as well as that in emergent (insular) rock, I hypothesize a much more cosmopolitan distribution of these shrimps in the Indo-Pacific Tropical Zone.

  17. Authenticating the origin of different shrimp products on the Tunisian ...

    African Journals Online (AJOL)

    KAIS

    2015-07-22

    Jul 22, 2015 ... methods (with various modifications) and the use of commercial kit such as Chelex ... Authenticity determination of prawn and shrimp species in commercial frozen, pre-cooked products. .... primers have equal length (23 nucleotides), GC content ..... Temperatures in Diffusion-Generated Chemical Gradients.

  18. 21 CFR 161.175 - Frozen raw breaded shrimp.

    Science.gov (United States)

    2010-04-01

    ... additives as defined in section 201(s) of the Federal Food, Drug, and Cosmetic Act; or if they are food... 21 Food and Drugs 2 2010-04-01 2010-04-01 false Frozen raw breaded shrimp. 161.175 Section 161.175 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED) FOOD...

  19. Multilevel analysis of elastic morphology: The mantis shrimp's spring.

    Science.gov (United States)

    Rosario, M V; Patek, S N

    2015-09-01

    Spring systems, whether natural or engineered, are composed of compliant and rigid regions. Biological springs are often similar to monolithic structures that distribute compliance and rigidity across the whole system. For example, to confer different amounts of compliance in distinct regions within a single structure, biological systems typically vary regional morphology through thickening or elongation. Here, we analyze the monolithic spring in mantis shrimp (Stomatopoda) raptorial appendages to rapidly acquire or process prey. We quantified the shape of cross-sections of the merus segment of the raptorial appendage. We also examined specific regions of the merus that are hypothesized to either store elastic energy or provide structural support to permit energy storage in other regions of the system. We found that while all mantis shrimp contain thicker ventral bars in distal cross-sections, differences in thickness are more pronounced in high-impact "smasher" mantis shrimp than in the slower-striking "spearer" mantis shrimp. We also found that spearer cross-sections are more circular while those of smashers are more eccentric with elongation along the dorso-ventral axis. The results suggest that the regional thickening of ventral bars provides structural support for resisting spring compression and also reduces flexural stiffness along the system's long axis. This multilevel morphological analysis offers a foundation for understanding the evolution and mechanics of monolithic systems in biology. © 2015 Wiley Periodicals, Inc.

  20. Levels of PAHs in shrimps, Penaeus monodon from Jones Creek ...

    African Journals Online (AJOL)

    High concentrations were recorded during the rainy season and during the first quarter of the year. The investigated samples were classified as minimally contaminated when compared with FDA levels of PAHs in shrimp samples. Distribution patterns showed that PAHs with 3 and 4 rings dominated confirming the pyrogenic ...