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Sample records for breve exegesis sobre

  1. Brief exegesis on the existing legislation and the application of thermal insulation in the world - proposal for Mexico; Breve exegesis sobre legislacion existente en la aplicacion de aislamientos termicos en el mundo - propuesta para Mexico

    Energy Technology Data Exchange (ETDEWEB)

    Ordonez C, Luis Felipe [Aislantes Minerales, S. A. de C. V., Mexico, D. F. (Mexico)

    1993-12-31

    In different countries of the world, derived from its great concern for energy conservation, the natural resources and the environmental preservation, and because of the high cost that represents the immoderate utilization of the energy and its secondary effects, legislation and regulations on the subject of the use of thermal insulation in commercial, industrial and dwelling buildings, have been approved. These actions have yielded very significant savings in reference to national macro-economies as well as in the individual family economies. As a direct consequence of the reduction of the energy consumption the emission of pollutants derived from combustion is proportionally reduced. The reduction is reflected in its daily expenses and transfers its benefits in direct and immediate form. Other secondary beneficial effects are: The quality of life of the individuals is noticeably improved in more comfortable dwellings, new jobs are generated as well as greater training needs to the construction personnel and the offer of choices on construction systems is widened. In first term a summarized and explained presentation is made on the legislation and regulations adopted in a wide list of different countries all over the world; leaders in this aspect. Afterwards, a presentation is made of the measures that could be implemented in Mexico and specific values of insulation, in accordance with the different climatological Mexican regions, are suggested. [Espanol] En distintos paises del mundo, derivado de su gran preocupacion por la conservacion de la energia, de los recursos naturales y de la preservacion del medio ambiente; por el alto costo que representa el uso desmedido de los energeticos y sus efectos secundarios, se han aprobado legislacion y reglamentacion en materia del uso de aislamientos termicos en edificaciones de tipo comercial, industrial y habitacional. Estas acciones han redituado ahorros muy significativos, tanto en referencia a las macro-economias nacionales como a las microeconomias familiares individuales. En consecuencia directa de la reduccion en el consumo energetico, se reduce en forma proporcional la emision de los contaminantes derivados de la combustion. La reduccion se realiza en su gasto diario y transfiere sus beneficios en forma directa e inmediata. Otros efectos secundarios beneficos son; se mejora sensiblemente la calidad de vida de los habitantes de viviendas mas confortables, se generan nuevos empleos y necesidades de mayor capacitacion al personal de la construccion y se amplia la oferta de alternativas de sistemas constructivos. En primer termino, se hace una presentacion resumida y explicada de la legislacion y reglamentacion adoptada en una amplia lista de distintos paises del mundo; lideres en este aspecto. Posteriormente, se hace una presentacion de las medidas que se pudieran implementar a este respecto en Mexico y se sugieren valores especificos de aislamiento relativos a las distintas regiones climatologicas mexicanas.

  2. Brief exegesis on the existing legislation and the application of thermal insulation in the world - proposal for Mexico; Breve exegesis sobre legislacion existente en la aplicacion de aislamientos termicos en el mundo - propuesta para Mexico

    Energy Technology Data Exchange (ETDEWEB)

    Ordonez C, Luis Felipe [Aislantes Minerales, S. A. de C. V., Mexico, D. F. (Mexico)

    1992-12-31

    In different countries of the world, derived from its great concern for energy conservation, the natural resources and the environmental preservation, and because of the high cost that represents the immoderate utilization of the energy and its secondary effects, legislation and regulations on the subject of the use of thermal insulation in commercial, industrial and dwelling buildings, have been approved. These actions have yielded very significant savings in reference to national macro-economies as well as in the individual family economies. As a direct consequence of the reduction of the energy consumption the emission of pollutants derived from combustion is proportionally reduced. The reduction is reflected in its daily expenses and transfers its benefits in direct and immediate form. Other secondary beneficial effects are: The quality of life of the individuals is noticeably improved in more comfortable dwellings, new jobs are generated as well as greater training needs to the construction personnel and the offer of choices on construction systems is widened. In first term a summarized and explained presentation is made on the legislation and regulations adopted in a wide list of different countries all over the world; leaders in this aspect. Afterwards, a presentation is made of the measures that could be implemented in Mexico and specific values of insulation, in accordance with the different climatological Mexican regions, are suggested. [Espanol] En distintos paises del mundo, derivado de su gran preocupacion por la conservacion de la energia, de los recursos naturales y de la preservacion del medio ambiente; por el alto costo que representa el uso desmedido de los energeticos y sus efectos secundarios, se han aprobado legislacion y reglamentacion en materia del uso de aislamientos termicos en edificaciones de tipo comercial, industrial y habitacional. Estas acciones han redituado ahorros muy significativos, tanto en referencia a las macro-economias nacionales como a las microeconomias familiares individuales. En consecuencia directa de la reduccion en el consumo energetico, se reduce en forma proporcional la emision de los contaminantes derivados de la combustion. La reduccion se realiza en su gasto diario y transfiere sus beneficios en forma directa e inmediata. Otros efectos secundarios beneficos son; se mejora sensiblemente la calidad de vida de los habitantes de viviendas mas confortables, se generan nuevos empleos y necesidades de mayor capacitacion al personal de la construccion y se amplia la oferta de alternativas de sistemas constructivos. En primer termino, se hace una presentacion resumida y explicada de la legislacion y reglamentacion adoptada en una amplia lista de distintos paises del mundo; lideres en este aspecto. Posteriormente, se hace una presentacion de las medidas que se pudieran implementar a este respecto en Mexico y se sugieren valores especificos de aislamiento relativos a las distintas regiones climatologicas mexicanas.

  3. Uma breve reflexão sobre a integralidade Una breve reflexión sobre la integralidad A brief reflection about integrality

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    Rosane Teresinha Fontoura

    2006-08-01

    Full Text Available O estudo teve como objetivo investigar o conhecimento dos profissionais enfermeiros acerca da Integralidade em Saúde, de maneira a refletir sobre a interface deste princípio nas relações de trabalho. Os sujeitos da pesquisa são enfermeiros da rede básica de dois municípios do Rio Grande do Sul. Coletaram-se dados através de um questionário. Na análise foram codificados 5 categorias: O SUS de hoje, os princípios do SUS, a integralidade da assistência, a integralidade e sua interface no cotidiano do trabalho dos enfermeiros e o atendimento integral como prioridade nos serviços. Para os respondentes o SUS é um sistema completo em seus princípios e diretrizes, porém, na realidade não funciona conforme legislação.Os entrevistados percebem a integralidade, como prioridade nas práticas de saúde.El estudio tuvo como objetivo explorar el conocimiento del enfermero sobre la Integralidad em Salud, para ser possible el reflejar sobre la interfacies de este principio en las relaciones laborales. Los sujetos de investigación son enfermeros de la red basica de salud de dos municipios de Rio Grande do Sul. Los datos fueron recolectados través de un cuestionario y analisados segín cinco categorias: el Sistema Unico de Salud (SUS de hoy, los principios del SUS, la integralidad de la atención, la integralidade y su interfacies en el cotidiano de los enfermeros y la atención integral como prioridad en los servicios. Para los respondentes el SUS es un sistema completo un sus principios y diretrices, pero en la realidad no funciona de acuerdo con la legislación. Los respondentes perciben la integralidad como prioridad en las prácticas de salud.The study aimed at exploring the knowledge of nurses about integrality in health, in such way to reflect about the interface of this principle in work relations. The research subjects were nurses from the basic health system of two counties of Rio Grande do Sul State. The data were collected by a

  4. Breves considerações sobre o "Conceito de ironia em Soren Kierkegaard".

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    Jacqueline Oliveira Leão

    2013-05-01

    Full Text Available Resumo: Este ensaio intenta discutir sobre o conceito de ironia em Søren Kierkegaard. Além disso, objetiva demonstrar a relação entre pensamento e palavra, proposição e significado. Palavras-chave: Ironia. Kierkegaard. Subjetividade.

  5. Speech act theory and New Testament exegesis

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    J. Botha

    1991-01-01

    Full Text Available Speech act theory offers New Testament exegesis some additional ways and means of approaching the text of the New Testament. This, the second in a series of two articles that make a plea for the continued utilisation and application of this theory to the text of the New Testament, deals with some of the possibilities and potential this theory holds for reading biblical texts. Advantages are pointed out and a few suggestions for the future proposed.

  6. UN BREVE PANORAMA GENERAL SOBRE LAS INTERVENCIONES DE LOS SISTEMAS: ADELGAZAMIENTO DE LA ESTRUCTURA ORGANIZACIONAL

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    Carlos Rodeiro

    2001-01-01

    Full Text Available Este documento tiene dos propósitos. Por un lado, se trata de revisar los diferentes enfoques hacia el problema del adelgazamiento de la estructura organizacional y sus efectos en el desempeño de una empresa. Es necesario prestar especial atención a la perspectiva socio-cognoscitiva propuesta por McKinley, Zhao y Rust (2000 y el enfoque de red seleccionado por Shah (2000 para comprender las perturbaciones que puede ocasionar el adelgazamiento de la estructura organizacional. Por otro lado, mi intención es contribuir a expandir la comprensión acerca del efecto de las intervenciones de los sistemas tanto en el desempeño de la empresa como en la conducta de cada persona. El cuerpo de esta revisión es el siguiente: Primero, comienzo por definir adelgazamiento de la estructura organizacional y hablar sobre las perspectivas que se usan para explicar los efectos del adelgazamiento (por ejemplo perspectivas económicas, institucionales y socio-cognoscitivas. Luego me desplazo hacia la literatura que trata sobre las consecuencias más comunes del adelgazamiento de la estructura organizacional, al igual que otros puntos de vista referentes a la relación entre a adelgazamiento y capacidad de aprendizaje organizacional, b adelgazamiento y entorno laboral para la creatividad, y c adelgazamiento y motivación laboral. Enseguida, considero las reacciones de los sobrevivientes a los procesos de adelgazamiento bajo una perspectiva de red. Finalmente, expongo algunas ideas para posterior investigación.

  7. Narrativas sobre a perda da visão: breves reflexões sobre corpo e emoções

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    Luiz Gustavo Pereira de Souza Correia

    2009-12-01

    Full Text Available Este texto tem como objetivo compreender as vivências dos processos simbólicos e as interpretações pessoais sobre a perda da visão via narrativas de indivíduos cegos habitantes de Porto Alegre, Rio Grande do Sul. As emoções expressas nas narrativas são os elementos de análise e compreensão das elaborações simbólicas dos personagens da sua localização e orientação social como indivíduos cegos. Desta forma é discutida a intersubjetividade na vivência da cegueira, como possibilidade de pensar a experiência subjetiva do corpo em seu engajamento ou enraizamento no mundo social. Fecha de recepción: 22 septiembre 2009. Fecha de aceptación: 02 de noviembre 2009.

  8. BREVES REFLEXÕES SOBRE A IMPRESCRITIBILIDADE DOS CRIMES DE RACISMO

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    Clarice Costa Calixto

    2010-08-01

    Full Text Available O artigo visa refletir sobre o dispositivo da Constituição brasileira de 1988 que prevê a imprescritibilidade dos crimes de racismo. Inicia-se com um panorama histórico da formação da sociedade brasileira, investigando-se as origens da discriminação no país e abordando a questão do conceito de raça como construção cultural, e não como grupo diferenciado por características biológicas. Após, é apresentado o tratamento jurídico da discriminação nas convenções internacionais e no ordenamento jurídico brasileiro, bem como as características centrais do instituto da prescrição penal. Finaliza-se o texto demonstrando as incongruências da previsão da imprescritibilidade do crime de racismo em relação às tendências garantistas e democráticas da ordem jurídica instaurada pelo texto constitucional vigente, tendo em vista o autoritarismo da eternização da possibilidade de punição e os fundamentos teóricos da prescrição penal relativos ao esquecimento, à dispersão das provas, à expiação moral, à emenda e à teoria psicológica.

  9. Breve revisión sobre características de la cutícula vegetal y penetración de herbicidas

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    María del C. Lallana

    2006-01-01

    Full Text Available Se presenta una breve revisión sobre las características de la cutícula de las plantas y su relación con la penetración de los herbicidas, así como los factores que afectan dicha penetración. Se tienen en cuenta aspectos estructurales, como características de las ceras de la cutícula, y ambientales que determinan la correcta absorción de los herbicidas. Se hace referencia a los estudios realizados sobre Eryngium horridum, la presencia de estomas y la penetración diferencial de los herbicidas en el haz y en el envés de las hojas.

  10. Comunicação de moda: um breve olhar sobre o desfile como ferramenta na contemporaneidade

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    Cynthia Hansen

    2013-05-01

    Full Text Available O objetivo desta pesquisa é refletir sobre a utilidade dos desfiles como ferramenta de comunicação para marcas e criadores de moda na contemporaneidade. A partir da investigação exploratória bibliográfica sobre o conceito, função e evolução histórica dos desfiles, bem como sobre argumentos de autores que refutam e que corroboram a utilidade desses eventos em vista da forma como são realizados hoje, foi possível verificar que os desfiles servem como ferramenta de comunicação mercadológica geradora de publicidade, uma vez que são colocados em circulação na mídia na tentativa de impressionar e buscar reconhecimento das marcas perante o público, tanto especializado quanto leigo.

  11. Breve nota sobre hábitos de dormir do tamanduá-bandeira - Myrmecophaga tridactyla Linnaeus (Xenarthra, Myrmecophagidae)

    OpenAIRE

    Medri, Ísis Meri; Mourão, Guilherme

    2005-01-01

    The scientific literature on giant anteaters states that the animal sleeps with its tail folded over its body to conserve body temperature. However, observations of this species in natural habitats indicate variations in this behavior, depending on the ambient temperature.A literatura científica sobre tamanduá-bandeira afirma que o animal dorme com sua cauda dobrada sobre o corpo para conservar a temperatura corporal. Entretanto, observações desta espécie em hábitats naturais indicam variaçõe...

  12. The potential of speech act theory for New Testament exegesis ...

    African Journals Online (AJOL)

    Speech act theory as well offers New Testament exegesis some additional ways and means of approaching the text of the New Testament. This first in a series of two articles making a plea for the continued utilisation and application of this theory to the text of the New Testament, offers a brief discussion of the basic ...

  13. Speech act theory and New Testament exegesis | Botha | HTS ...

    African Journals Online (AJOL)

    Speech act theory offers New Testament exegesis some additional ways and means of approaching the text of the New Testament. This, the second in a series of two articles that make a plea for the continued utilisation and application of this theory to the text of the New Testament, deals with some of the possibilities and ...

  14. Exegesis and Hermeneutics in Erasmus' Paraphrase on Luke

    NARCIS (Netherlands)

    Bloemendal, J.

    2016-01-01

    This article discusses how Erasmus deals with the personae in the Paraphrase on Luke, distinguishing between the author Erasmus (the paraphrast), the narrator (the paraphraser) and the evangelist. These ‘Luke-voices’ are connected to exegesis (explanation of the narrative) and hermeneutics (the

  15. Metodologia de pesquisas na internet: breves considerações sobre uma pesquisa qualitativa em turismo nas redes sociais

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    Fernanda Meneses de Miranda Castro

    2015-08-01

    Full Text Available Este artigo teve por objetivo apresentar o percurso metodológico de uma pesquisa que pretendeu analisar a contribuição das redes sociais que são utilizadas pelos órgãos responsáveis pela promoção do turismo de Salvador para a construção de uma imagem competitiva do destino. Com o surgimento da comunicação virtual surgiram novas propostas de pesquisa que tentassem desvendar esse ambiente e ainda não existe um método que seja considerado mais adequado para o alcance dos objetivos, cabendo ao pesquisador a tarefa de selecionar métodos e técnicas que mais se ajustem à proposta de pesquisa. Neste trabalho optou-se pela utilização do método sociológico e pelas técnicas de análise das redes sociais, iconologia e análise de conteúdo. Apresentou-se também breves resultados da pesquisa empírica, realizada entre agosto de 2013 a agosto de 2014, onde acompanhou-se as publicações referentes ao destino Salvador da página da rede social Facebook da Bahiatursa e da Salvador-Bahia. Os resultados indicaram que apesar de Salvador ser o destino âncora da Bahia, a promoção digital ainda não alcançou resultados expressivos. Indicaram também que os métodos e técnicas escolhidos atenderam ao objetivo proposto e que pesquisas científicas na Internet requerem do pesquisador uma análise contextual pormenorizada. A depender do objeto e objetivos de estudo, a escolha e o percurso metodológico são diferenciados, não havendo uma indicação conceitual priorizada.

  16. Breve reflexión sobre la experiencia y el cuerpo/Brief reflection on the experience and the body

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    Raumar Giménez Rodríguez

    2007-01-01

    Full Text Available El concepto de experiencia es, en principio, vasto en demasía como para proponerse un estudio profundo en poco tiempo. Por ello, en estas breves reflexiones que constituyen una de las trazas del recorrido iniciado a propósito de la relación cuerpo-lenguaje-enseñanza, la noción de experiencia es referida en primer lugar desde el texto homónimo escrito por Walter Benjamin en 1913 y luego desde las reflexiones que despliega Agamben en su “Infancia e historia”, escrito en 1978. A partir de allí, se presentan algunas ideas en las que se exploran las posibilidades que brinda la noción de experiencia para problematizar algunos rasgos específicos de la historia del cuerpo en la modernidad, su relación con una forma específica de producción de conocimiento (la ciencia moderna y los avatares de la experiencia corporal en la formación social capitalista. Para esta reflexión, se ha supuesto que para que acontezca algo del orden de lo educativo y de la enseñanza y que ello, a su vez, sea pensado desde la cuestión del cuerpo, es preciso una experiencia. El carácter exploratorio del trabajo tal vez no permitirá más que una delimitación rigurosa del problema de estudio. Para ello, se introducen tópicos teóricos de distinta procedencia que se ponen en juego a tales efectos. The concept of experience is, in itself, too vast to propose its deep study in a short period of time. That is the reason why, in these brief reflections that constitute one of the traces of the course initiated about the relation body-language-teaching, the notion of experience is referred to, in the first place, from the homonym text written by Walter Benjamin in 1913 and then from the reflections displayed by Agamben in his" Infancy and History ", written in 1978. From that point, some ideas are presented where the possibilities given by the notion of experience, to question some specific features of the history of the body in Modernity are explored, its

  17. “Historiografía sobre la masonería en México. Breve revisión”

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    María Eugenia Vázquez Semadeni

    2010-01-01

    Full Text Available Este texto procura mostrar las principales líneas seguidas por los estudios sobre la masonería mexicana. En un primer apartado menciona las obras fundamentales de la historiografía no académica, en la que se incluyen los textos masónicos, antimasónicos y de divulgación, procurando destacar la importancia y utilidad, que algunos de estos trabajos tienen para el historiador profesional. Después revisa las producciones académicas, divididas en cuatro apartados, dedicados a los cuatro momentos políticos sucesivos en que la historiografía ha abordado el tema masónico: 1 la independencia y las primeras repúblicas; 2 la reforma y la república restaurada; 3 el Porfiriato; y 4 la revolución y el periodo posrevolucionario.

  18. DOCUMENTO: BREVES REFLEXIONES SOBRE LA INVESTIGACIÓN CUALITATIVA PARA LA FORMACIÓN DEL TRABAJADOR SOCIAL

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    Nilsa Burgos Ortiz

    2009-08-01

    Full Text Available Este documento presenta, en primera instancia, un resumen sobre la trayectoria de la investigación social en América, en su relación con la disciplina de Trabajo Social; y en segunda instancia, una experiencia de aplicación de la investigación cualitativa en un estudio adelantado por el primer grupo de estudiantes doctorales en la Escuela Graduada de Trabajo Social de la Universidad de Puerto Rico. Este grupo estudió la violencia en una escuela pública del área metropolitana de San Juan y examinó una de las políticas sociales pertinente desde la perspectiva teórica del construccionismo social. El grupo de estudiantes se organizó en cuatro equipos de trabajo y utilizó entrevistas cualitativas, grupos focales y la observación para la recopilación de datos. El ejercicio permitió concluir que la aplicación de la metodología cualitativa para el estudio de problemas sociales, así como para el análisis de política social es de gran relevancia en la práctica profesional del trabajo social.

  19. Crianças negras na história: Fontes e discursos sobre a breve infância permitida pelo escravismo oitocentista brasileiro

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    Ione da Silva Jovino

    2015-08-01

    Full Text Available The article presents part of historical research on children, childhood and race in the 19th century iconography. The main aim of the article is to discuss how children and black childhood were represented in a number of pictorial materials. Focusing on childhood history and its interfaces with Brazilian slavery history, the article presents a brief introduction about the sources and the research field. The analysis aims to show traces of black childhood in the 19th century, from travel literature to memory journals. Some aspects of children’s life, such as play and education point out that, even in a limited and restricted way, it was possible to demonstrate that children experienced childhood moments; it was also possible to envision their form of resistance to adult impositions. Some reflections about education are exposed before discussing the relation between childhood and work and the presentation of children´s perceptions of slavery, collected from literary texts. The final considerations are based on the analysis of a short story written at the beginning of the 20th century, which still presents traces of slavery by showing an enslaved black child, despite out of formal slavery. Despite the harshness of slavery, the article considers the possibility of a playful childhood, which is mediating, playful, challenging, made up of few moments of satisfaction among toys, games, pranks and transgressions. O artigo apresenta recortes de uma pesquisa histórica sobre criança, infância e raça na iconografia do século XIX, sendo a discussão sobre a maneira como eram representadas crianças e infâncias negras em diversos materiais imagéticos o objetivo principal. Com olhar centrado na história da infância e suas interfaces com a da escravidão no Brasil, o texto começa por apresentar breve introdução sobre as fontes e campos utilizados na pesquisa. A análise busca evidenciar vestígios da infância negra no século XIX, iniciando com

  20. Exegesis seeking appropriation; appropriation seeking exegesis: Re-reading 2 Samuel 13:1−22 in search of redemptive masculinities

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    Gerald West

    2013-09-01

    Full Text Available Exegesis in the traditional sense is concerned with generating as much (scientific detail about a biblical text as possible. Whilst the two primary modes of biblical exegesis � socio-historical and literary-semiotic � do this differently, they share a common concern for the detail of the text as an ancient artefact. Critical distance is a key concept here, with the exegetes bracketing (for a moment their own contexts and concerns. However, such bracketing is impossible to sustain, and so the exegetes� interests (shaped by their contexts and concerns �leak� into the act of exegesis. Most exegetes today recognise this leakage, and whilst some still view such leakage as contaminating the exegesis, others, including the tradition of African biblical scholarship, actively identify the contextual concerns they bring to the task of exegesis, both respecting the detail of the text and desiring to be accountable to their contexts in which the Bible is a significant text. This article explored some of the dimensions of forms of exegesis that actively seek appropriation, using 2 Samuel 13:1�22 as an example. In this case, the article analysed the contextual shift from a focus on women as the victims of sexual violence to an emerging emphasis on masculinities. Reading the same text from these different contextual concerns �activates� particular details of the text, and so both draw on different elements of the text and thus guides the gaze of exegesis.

  1. Revisão de literatura sobre a aplicação da entrevista motivacional breve em usuários nocivos e dependentes de álcool Una revisión de la literatura sobre la aplicación de la entrevista motivacional breve en abuso o dependencia del alcohol A literature revision about the application of the brief motivational interviewing in harmful users and alcohol dependents

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    Cristiane Martins Baía Sales

    2009-06-01

    Full Text Available Este artigo apresenta os elementos necessários para a compreensão do uso da Entrevista Motivacional Breve em usuários nocivos e dependentes de álcool através de uma revisão da literatura sobre sua estrutura metodológica e efetividade. O edstudo inclui a revisão e discussão sobre o uso desta abordagem no Brasil. Foram selecionados 18 estudos internacionais e nacionais e seus resultados foram discutidos a partir da apresentação de duas tabelas com os principais resultados. A Entrevista Motivacional Breve consiste numa abordagem eficaz para usuários nocivos e dependentes de álcool; possui uma metodologia prática e objetiva, que permite ser ela aplicada por qualquer profissional treinado, podendo ser utilizada sozinha, acoplada a outras técnicas ou mesmo como prelúdio, preparando uma base motivacional para outros tratamentos. No Brasil m ais pesquisas são necessárias para que se encontrem meios eficientes, eficazes e de baixo custo para tratar indivíduos que se encontrem e qualquer dos diferentes estágios desta doença.Este artículo presenta los elementos necesarios para la comprensión del uso de la Entrevista Motivacional Breve en usuarios nocivos y dependientes de Alcohol a través de una revisión de la literatura sobre la estructura de su metodología y efectividad. Este trabajo también incluye la revisión y discusión sobre el uso de este abordaje en Brasil. Fueron seleccionados 18 estudios internacionales y nacionales y sus resultados fueron discutidos a partir de la presentación de sus tablas con los principales resultados. La Entrevista Motivacional Breve consiste en un abordaje eficaz para usuarios nocivos y dependientes del alcohol, posee metodología práctica y objetiva que permite ser aplicada por cualquier profesional entrenado, podiendo ser utilizada sola, acoplada a otras técnicas o aun como preludio preparando una base motivacional para otros tratamientos. En Brasil más pesquisas son necesarias para que se

  2. CONSIDERAÇÕES SOBRE AS AÇÕES COLETIVAS PASSIVAS NO BRASIL E NA INGLATERRA: UM BREVE ESTUDO COMPARADO

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    Neto, Zaiden Geraige; Bulos, Jaime Leandro; Lima, Marcelo Augusto de Toledo

    2014-01-01

    O presente artigo visa traçar um paralelo sobre o instituto das ações coletivas passivas no Brasil e na Inglaterra, demonstrando assim as semelhanças e diferenças existentes em cada país sobre a aplicação de referido instituto, bem como as suas vantagens e desvantagens em nosso ordenamento diante do contexto jurídico da atualidade.

  3. Brazilian soap opera and cultural industry: a brief essay about Reynaldo Gianecchini's character in Sete Pecados Telenovela Brasileira e indústria cultural: um breve ensaio sobre o personagem de Reynaldo Gianecchini em Sete Pecados

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    Márcio Ferreira Rodrigues Pereira

    2008-12-01

    Full Text Available The soap opera, first made in Brazil in 1951, became famous throughout time as one of the most important cultural products of our country’s television. With the analysis of a chosen case, the character “Dante” played by the actor Reynaldo Gianecchini in the Rede Globo soap opera “Sete Pecados” (2007 – 2008, supported by some theory of the mass culture, we try to launch a critical look over this powerful cultural product. But, before, with the intention of offering view of the current Brazilian TV drama, a brief history of the beginning, developing and narrative of the soap opera is presented. Keywords: Theories of mass culture. Cultural industry. Brazilian soap opera. A telenovela, surgida no Brasil em 1951, consagrou-se ao longo do tempo como um dos produtos culturais mais importantes da televisão nacional. Através da análise de um caso escolhido, o personagem “Dante”, interpretado pelo ator Reynaldo Gianecchini, da telenovela da Rede Globo Sete pecados (2007 – 2008, busca-se, com apoio em alguns teóricos da cultura de massa, lançar um olhar crítico sobre este poderoso produto cultural. Antes, porém, com o fi to de oferecer um panorama da teledramaturgia brasileira atual, apresenta-se um breve histórico sobre o surgimento, o desenvolvimento e a narrativa da telenovela. Palavras-chave: Teorias da cultura de massa. Indústria cultural. Telenovela brasileira.

  4. Breve historia de la investigación sobre biología celular en la Universidad de Buenos Aires durante el siglo XX

    OpenAIRE

    Solari, Alberto Juan

    2017-01-01

    Las investigaciones científicas sobre Biología Celular (anteriormente llamada Citología) se desarrollaron vigorosamente durante el siglo XX en la Argentina y, en particular, en la UBA. Los vaivenes de dicho desarrollo, a su vez, muestran una interesante correlación con los fenómenos sociales que ocurrieron en el país en ese siglo. En la actualidad (año 2014) la investigación argentina en esta disciplina ya no ocupa, lamentablemente, un lugar tan distinguido como el que tuvo en cierto periodo ...

  5. BREVES CONSIDERAÇÕES SOBRE A ABORDAGEM DE CHARLES GUIGNON DO MÉTODO FENOMENOLÓGICO EM SER E TEMPO

    Directory of Open Access Journals (Sweden)

    Rogério Tolfo

    2011-05-01

    Full Text Available Este artigo apresenta os estágios do método filosófico em Ser e Tempo, deacordo com a obra Heidegger e o Problema do Conhecimento de Charles Guignon.Segundo Guignon, Heidegger realiza em Ser e Tempo um processo em etapas: 1.Descritivo; 2. Hermenêutico; 3. Processo Dialético; 4. Diagnóstico do Senso Comum. Oobjetivo principal deste artigo é divulgar a proposta de Guignon. No entanto, faremosbreves considerações sobre esta proposta.

  6. Breve histórico do pensamento psicológico brasileiro sobre relações étnico-raciais

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    Alessandro de Oliveira dos Santos

    Full Text Available O artigo descreve três momentos do pensamento psicológico brasileiro sobre relações étnicoraciais no final do século XIX e no início do século XX, caracterizado pela consolidação da Escola Nina Rodrigues, que investiga características psicológicas dos escravos e ex-escravos e fornece elementos para a configuração do negro como sujeito psicológico; o período de 1930 até 1950 é caracterizado pelo debate da construção sociocultural das diferenças e da desconstrução do determinismo biológico das raças, e o período de 1990 em diante, pelos estudos sobre branqueamento e branquitude. Utilizando a perspectiva histórica, foi possível traçar uma linha no tempo identificando esses momentos de ruptura e de configuração de novos saberes em Psicologia: biológico-causal, culturalista e relacional. A construção dessa linha no tempo configura uma tentativa de trazer para o campo da Psicologia uma outra perspectiva de discussão da temática étnico-racial, repensando o papel dos modelos e das teorias psicológicas.

  7. Breve histórico do pensamento psicológico brasileiro sobre relações étnico-raciais

    OpenAIRE

    Santos,Alessandro de Oliveira dos; Schucman,Lia Vainer; Martins,Hildeberto Vieira

    2012-01-01

    O artigo descreve três momentos do pensamento psicológico brasileiro sobre relações étnicoraciais no final do século XIX e no início do século XX, caracterizado pela consolidação da Escola Nina Rodrigues, que investiga características psicológicas dos escravos e ex-escravos e fornece elementos para a configuração do negro como sujeito psicológico; o período de 1930 até 1950 é caracterizado pelo debate da construção sociocultural das diferenças e da desconstrução do determinismo biológico das ...

  8. Breves comentários sobre a questão da tortura enquanto prática imprescritível e violadora dos direitos humanos

    Directory of Open Access Journals (Sweden)

    William Eufrásio Nunes Pereira

    2015-11-01

    Full Text Available A tortura, enquanto ato de impor a terceiros sofrimentos físicos e/ou psicológicos para obtenção de respostas a questões que se deseja respondidas, sempre foi uma forma corriqueira entre os humanos. Esse ato se tornou mais danoso quando assumiu a dimensão institucionalizada por parte do leviatã. Forma usual, mas em decadência com a ocorrência do desenvolvimento social, político, econômico e jurídico do Século XX. Esse trabalho apresenta uma sucinta discussão sobre a tortura enquanto violação dos direitos humanos. Mostra que contra essa violação os organismos internacionais elaboraram convenções, normas, tratados e protocolos que visam  reduzir, quando não extinguir a pratica de atos violentos contra os direitos da humanidade, em especial, contra a vida. Relata que o Brasil assinou as convenções, mas vem apresentando uma sensível lentidão na implementação de mecanismo que efetivamente coíbam a pratica da tortura. No entanto, os mecanismos criados têm forçado ao ajustamento das normas e mecanismos internos as demandas sociais potencializados pelos foros internacionais, em particular, a Corte internacional de Direitos Humanos. A metodologia constituiu-se de uma pesquisa bibliográfica em livros e artigos sobre o tema em tela. Conclui com a constatação de que ainda irá demorar muito para o Brasil ajustar e tornar efetiva a ação dos mecanismos de combate a tortura, garantindo a plenitude dos direitos da humanidade, em especial o de não ser torturado.

  9. El espacio catalán de relaciones laborales: breve reflexión sobre el modelo de participación institucional

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    Miguel Ángel Catalinas

    2014-07-01

    Full Text Available La actual situación económica ha reabierto el debate sobre la reforma de la administración pública, concretamente desde la vertiente del gasto que realiza y el tipo de servicios que debe seguir prestando o no. Asimismo, se han promovido diversos estudios e informes que cuestionan la viabilidad de determinados organismos públicos, con el objetivo de buscar una mejor eficiencia y evitar duplicidades. En este sentido, se plantea la reflexión sobre tres organismos que forman parte del marco de relaciones laborales de Catalunya (El Consejo de Relaciones Laborales, El Tribunal Laboral de Catalunya y el Consejo de Trabajo, Económico y Social de Catalunya, con el fin de explorar posibles modelos institucionales alternativos, que se construiran aplicando la metodología de los MOE’s (modelos de orientación estratégica, que se ajusten mejor a la realidad económica y laboral y que, en todo caso, den respuesta a la necesidad de establecer estructuras organizativas más eficientes. The current economic situation has reopened the debate on the reform of public administration, particularly from the perspective of the expenditure carried out and the type of services that must continue being offered or not. Several studies and reports have been also tackling the discussion on the viability of certain public bodies in order to achieve better efficiency and avoid duplications. We focus our study on three institutions that comprise the institutional frame of labour relations in Catalonia (Labour Relations Board, the Labour Court of Catalonia, Economic and Social Council of Catalonia, models will be designed using “MOE”’s methodology (Strategic Orientation Models in order to explore alternative institutional frames that may fit properly in economic and labor current context and also respond to requirements for more efficient organizational structures.

  10. Divulgación científica: Enfoque por competencias en la enseñanza superior. Breves reflexiones sobre la evaluación

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    Viviana Cámara

    2010-12-01

    Full Text Available En la Universidad la evaluación de los aprendizajes constituye una problemática que actualmente sigue siendo una tarea pendiente. Los estudios innovadores sobre evaluación educativa tanto la evaluación formativa (Coll y Onrubia, 1999 como la evaluación auténtica (Trillo Alonso, 2005 y en particular la evaluación de competencias (Barberá, 2005 responden a una lógica educativa que se construye sobre evidencias y sus abordajes permiten trascender de los problemas métricos de rendimiento y de acreditación para acercarse a los objetivos formativos de aprendizaje y desarrollo. La evaluación auténtica propone una nueva forma de concebir los procesos de enseñanza, de aprendizaje y de evaluación. Todos estos sistemas plantean numerosos instrumentos- portafolios, proyectos, carpeta de evaluación; etc.- que son utilizados para exponer el nivel de progreso en los alumnos en determinadas habilidades cognitivas especí- ficas a una disciplina o bien valúan el desarrollo progresivo de competencias complejas, generales y/o profesionales. Las autoras, en este trabajo, presentan el diseño y los resultados obtenidos de la aplicación de un portafolio creado para obtener evidencias y vivencias de un grupo de estudiantes de la cátedra de Matemática Básica de la Facultad de Ciencias Económicas de la Universidad Nacional del Litoral en una unidad disciplinar durante un período de tiempo y, a su vez, un análisis a priori de la factibilidad de aplicación del portafolio en el área de Matemática

  11. Liderazgos guaraníes: breve revisión histórica y nuevas notas sobre la cuestión

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    Ana María Gorosito Kramer

    2006-08-01

    Full Text Available La interpretación propuesta por P. Clastres en su muy conocido artículo "Intercambio y poder: filosofía de la jefatura indígena" (En "La sociedad contra el Estado", acerca de la selección de los liderazgos indígenas en América del Sur -y especialmente en las Tierras Bajas Sudamericanas- intentó dar cuenta de una concepción de las jefaturas dinámica y fluida, cuyo fundamento respondía a las modalidades del intercambio y la redistribución. Sin embargo, el paulatino proceso de cooptación de líderes y manipulación de los mecanismos de selección de jefaturas, producto del avance de los frentes regionales y nacionales de expansión sobre áreas indígenas, ha operado y aún lo hace a través de su cristalización y congelamiento. Estos procesos exógenos de construcción de jefaturas apelan, sin embargo, a un supuesto respeto de la organización socio-política indígena, citando inclusive fuentes etnográficas para respaldar su accionar. Esta ponencia no se encamina a sumar una nueva revisión crítica del texto de Clastres, ya excesivamente analizado y puesto en cuestión, sino a una revisión de las intervenciones externas a la sociedad guaraní, considerando muy especialmente las que se han verificado en Misiones (Argentina desde 1960 a la actualidad.The interpretation proposed by P. Clastres in his very known article "Interchange and power: philosophy of the indigenous headquarters "(In the society against the State, about the selection of the indigenous leaderships in South America - specially in the South America Low lands - it tried to give account of a dynamic and fluid conception of headquarters, whose foundation responded to modalities of the interchange and redistribution. Nevertheless, the gradual process of cooptation of leaders and the manipulation of mechanisms of headquarters 's selection, product of the advance of regional and national fronts of extension on indigenous areas, has operated -and still does-, through his

  12. Breve noticia sobre el cuento en Colombia

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    Nestor Madrid Malo

    1968-10-01

    Full Text Available Tal vez ningún sector de la literatura colombiana ha sido objeto de menos estudio que el cuento. A la novela y a la poesía -al teatro incluso, no obstante su escaso cultivo entre nosotros- se han dedicado múltiples ensayos, que facilitan así ampliamente la tarea de quien deba presentar su línea histórica o su estado actual. Pero cosa bien distinta sucede con el cuento, género de muy reciente trayectoria en el país.

  13. Fotografando a geringonça sem freio: breve investigação sobre as transformações da música em Brasília desde os anos 80

    OpenAIRE

    Fernandes Cunha, André Luiz

    2018-01-01

    Inspirado na imagem de uma carroça futurista, criada pela banda Feijão de Bandido em 2001, esse artigo pretende fazer um breve mapeamento da cena musical em Brasília dos anos 80 até a atualidade considerando sua natureza híbrida: arcaica e moderna, global e local simultaneamente. 

  14. The potential of speech act theory for New Testament exegesis: Some basic concepts

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    J. E. Botha

    1991-01-01

    Full Text Available Exegetes and biblical scholars are increasingly utilising the precepts of modern literary and linguistic theories in dealing with the text of the Bible. Speech act theory as well offers New Testament exegesis some additional ways and means of approaching the text of the New Testament. This first in a series of two articles making a plea for the continued utilisation and application of this theory to the text of the New Testament, offers a brief discussion of the basic principles of the theory.

  15. Corporate religion og Paulus' breve

    DEFF Research Database (Denmark)

    Nielsen, Jesper Tang

    2007-01-01

    , analyseres grundigt. Af undersøgelsen fremgår det, at Kundes opfattelse af religion svarer til Clifford Geertz’ klassiske kulturantropologiske religionsdefinition. Ydermere fremdrages en række paralleller til Paulus’ breve, der godtgør, at Kundes forståelse og brug af religiøse forestillinger kan ses som en...

  16. A brief note on the sleeping habits of the giant anteater - Myrmecophaga tridactyla Linnaeus (Xenarthra, Myrmecophagidae Breve nota sobre hábitos de dormir do tamanduá-bandeira - Myrmecophaga tridactyla Linnaeus (Xenarthra, Myrmecophagidae

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    Ísis Meri Medri

    2005-12-01

    Full Text Available The scientific literature on giant anteaters states that the animal sleeps with its tail folded over its body to conserve body temperature. However, observations of this species in natural habitats indicate variations in this behavior, depending on the ambient temperature.A literatura científica sobre tamanduá-bandeira afirma que o animal dorme com sua cauda dobrada sobre o corpo para conservar a temperatura corporal. Entretanto, observações desta espécie em hábitats naturais indicam variações neste comportamento, dependendo da temperatura ambiente.

  17. Ventriculoperitoneal shunt infection caused by Bifidobacterium breve.

    Science.gov (United States)

    Suwantarat, Nuntra; Romagnoli, Mark; Wakefield, Teresa; Carroll, Karen C

    2014-08-01

    Bifidobacterium breve is a rare cause of human infections. Previously, bacteremia and meningitis caused by this organism linked to probiotic use have been reported in a neonate. We report the first case of a ventriculoperitoneal shunt infection caused by B. breve in an adult without a history of probiotic use. Copyright © 2014 Elsevier Ltd. All rights reserved.

  18. Cellodextrin utilization by bifidobacterium breve UCC2003.

    NARCIS (Netherlands)

    Pokusaeva, K.; Motherway, M.O.; Zomer, A.L.; Macsharry, J.; Fitzgerald, G.F.; Sinderen, D. van

    2011-01-01

    Cellodextrins, the incomplete hydrolysis products from insoluble cellulose, are accessible as a carbon source to certain members of the human gut microbiota, such as Bifidobacterium breve UCC2003. Transcription of the cldEFGC gene cluster of B. breve UCC2003 was shown to be induced upon growth on

  19. Na vida dez, na escola dez: breve discussão crítica acerca de pressupostos psicológicos e seus desdobramentos sobre a avaliação em Matemática escolar

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    Jorge Tarcísio da Rocha Falcão

    2010-05-01

    Full Text Available O presente artigo busca oferecer subsídios, oriundos da Psicologia, à discussão acerca da avaliação de competências cognitivas em Matemática. Os modelos atuais de avaliação escolar em geral, e avaliação matemática em particular, repousam, necessariamente, sobre concepções acerca de um sujeito humano fazendo Matemática. Tais concepções apóiam-se sobre modelos para o funcionamento cognitivo humano, em articulação com aspectos referentes à própria organização da Matemática, enquanto domínio de saber sócio-historicamente organizado e epistemologicamente circunscrito. Os modelos recentes fornecidos pela Psicologia da inteligência são aqui revistos, procurando-se evidenciar suas contribuições e limitações. Conclui-se, contudo, defendendo-se que nenhuma avaliação pode prescindir de tais modelos, mesmo que nenhum deles possa almejar a condição de definitivos, apresentando-se aqui pontos considerados cruciais para o delineamento de perfil de referência desejável de aprendiz de matemática, como referência para a avaliação da competência Matemática.

  20. Breves reflexões sobre a soberania e a supranacionalidade nos procesos de integração regional - 10.5102/uri.v8i2.1161

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    Elisa de Sousa Ribeiro

    2010-12-01

    Full Text Available O presente artigo tem como objetivo analisar uma realidade que emergiu no mundo globalizado: o debate acerca da (impossibilidade de concessão de parcela de soberania dos Estados para um organismo supranacional de integração. Com base nos discursos de cientistas políticos e internacionalistas, apresentam-se diferentes perspectivas sobre a afirmativa de que a soberania seria um atributo exclusivo do Estado e que esse não poderia ceder uma parcela a outro ente. Em outras palavras, procura-se refletir se o Estado efetivamente cede o exercício de algumas competências soberanas aos organismos de integração ou se a soberania poderia ser compartilhada ou até mesmo concedida parcial ou totalmente a um organismo internacional.

  1. BREVE ESTUDO DO ESTADO DA ARTE SOBRE ACIDENTE VASCULAR CEREBRAL E SERIOUS GAMES PARA APLICAÇAO NO PROJETO “AVC” DO NÚCLEO DE TECNOLOGIA ASSISTIVA DA UFS

    Directory of Open Access Journals (Sweden)

    Saulo Lordão Andrade Barros

    2013-03-01

    Full Text Available O Acidente Vascular Cerebral é uma das principais causas de morte e de sequelas no mundo. A doença atinge cerca de 16 milhões de pessoas anualmente, dessas, seis milhões morrem. No Brasil, é a principal causa de morte e incapacidade, gerando impactos econômicos e sociais. O trabalho de recuperação desses pacientes é de suma importância para diminuir os impactos causados. Para isto, o presente artigo traz uma revisão da literatura existente sobre a doença e seu tratamento, com foco na utilização de novas tecnologias, como o uso de jogos eletrônicos sérios, a fim de aprimorar o trabalho de recuperação tanto no âmbito físico quanto no emocional.

  2. Realidad aumentada en fisiología: descarga y breves instrucciones del uso de la aplicación HP REVEAL (AURASMA) sobre marcadores de nivel 1 y 2 en la docencia

    OpenAIRE

    Domínguez Vías, Germán; Carrasco Viñuela, Manuel; González Forero, David; Portillo Pacheco, Federico Luís; Vallo de Castro, Jose Juan

    2018-01-01

    Diálogo (Script): - Hola a todos. Una vez descargada la aplicación Aurasma, ahora llamada HP Reveal, tendréis que pulsar la aplicación, app AURASMA, y buscar y darle a seguir al grupo fisiologiaUCA. A continuación, podréis probar en clase activando el escáner y enfocando sobre marcadores de realidad aumentada de nivel 1, como código, o al final de este video marcadores de nivel 2, como son las fotos. Os saldrán, como estáis viendo, animaciones que os ayudarán a ver y comprender conceptos ...

  3. Yahweh’s ‘lord’ and Unrestrained Evil: An Exegesis of Psalm 110

    Directory of Open Access Journals (Sweden)

    Aran J.E. Persaud

    2015-08-01

    Full Text Available This article consists of an exegetical investigation of Psalm 110 and assumes that the psalms function in one sense as prayers, which can be prayed authentically by modern worshippers. Consequently, the specific images of hostile acts of war present a challenge as prayer. The methodology involves the investigation of the text from a grammatico-historical perspective by using selected sources from the 19th century to the present. The meaning of the violent images of enmity and the defining characteristics of the enemy are determined through understanding the perceived suffering of the psalmist. Special attention is given to the role of the phrase םֻאְנָּהוהְי, the identification of יִֹנדַאל and defining the enemy. The conclusions of the exegesis support a more traditional interpretation of יִֹנדַאל and characterise the recalcitrant enemies in an eschatological framework. Jahwe se ‘heer’ en onbeteuelde boosheid: ’n Eksegese van Psalm 110. Hierdie artikel is ’n eksegetiese ondersoek van Psalm 110 en gaan van die veronderstelling uit dat die psalms in ’n sekere sin gebede is wat deur moderne gelowiges gebid kan word. Die spesifieke uitbeeldings van vyandige oorlogsdade stel egter ’n uitdaging aan dié gebed. Die metodologie behels die ondersoek van die teks vanuit ’n grammaties-historiese perspektief deur uitgesoekte bronne vanaf die negentiende eeu tot tans te gebruik. Die betekenis van die geweldadige uitbeelding van vyandigheid en die omskrywende karaktertrekke van die vyand word bepaal deur die verstaan van die waarneembare lyding van die psalmskrywer. Daar word besondere aandag gegee aan die rol van die frase ָהוְהיּםֻאְנ, die eiening van יִֹנדַאל en die omskrywing van die vyand. Die slotsom van die eksegesis ondersteun ’n meer tradisionele interpretasie van יִֹנדַאל en tipeer die onversetlike vyand in ’n eskatalogiese raamwerk.

  4. Apuntes sobre esporotricosis

    OpenAIRE

    Londoño, Fabio

    2011-01-01

    Se hace una breve reseña histórica de las primeras comunicaciones sobre esporotricosis. Se destaca la amplia difusión de la enfermedad en nuestro país. 3. Se hace notar la no existencia de datos bibliográficos sobre formas extracutáneas. 4. Se llama la atención sobre la eficaz ayuda de la intradermo-reacción con esporotriquina en el diagnóstico de la enfermedad. 5- Se comunica un caso de esporotricosis verrugosa tratado con anfotericin B, con excelentes resultados

  5. Apuntes sobre esporotricosis

    Directory of Open Access Journals (Sweden)

    Fabio Londoño

    1960-01-01

    Full Text Available Se hace una breve reseña histórica de las primeras comunicaciones sobre esporotricosis. Se destaca la amplia difusión de la enfermedad en nuestro país. 3. Se hace notar la no existencia de datos bibliográficos sobre formas extracutáneas. 4. Se llama la atención sobre la eficaz ayuda de la intradermo-reacción con esporotriquina en el diagnóstico de la enfermedad. 5- Se comunica un caso de esporotricosis verrugosa tratado con anfotericin B, con excelentes resultados

  6. Da democracia participativa à pluralidade da representação: breves notas sobre a odisseia do PT na política e na ciência política brasileira

    Directory of Open Access Journals (Sweden)

    Bruno P. W. Reis

    2014-04-01

    Full Text Available Este artigo parte da constatação de um deslocamento do lugar ocupado pela ideia da representação na esquerda contemporânea para embasar um diagnóstico da experiência do PT na história política recente no Brasil. Embora tivesse partido de uma contestação da "democracia representativa", o PT terminou por patrocinar o mais vasto experimento de multiplicação de formas e instâncias de representação em curso no Brasil contemporâneo, frequentemente sob a rubrica de um ideal "participativo". O artigo tende a sublinhar o caráter inapelável dessa "deriva" rumo à representação, bem como identificar a raiz desses experimentos num compromisso político do partido com certos setores até então periféricos da população, bem mais que numa presumível clareza ideológica quanto a diagnósticos teoricamente orientados sobre o Brasil.

  7. Immune effects of the probiotic Bifidobacterium breve

    NARCIS (Netherlands)

    Ezendam J; Loveren H van; TOX

    2007-01-01

    Bifidobacterium breve, a probiotic, has beneficial effects on both allergy and autoimmunity - an immune reaction against the body's own constituents -in experimental animals. Probiotics are called 'friendly bacteria' in advertisements, in which manufacturers claim their beneficial effects on gut

  8. Immune effects of the probiotic Bifidobacterium breve

    NARCIS (Netherlands)

    Ezendam J; van Loveren H; TOX

    2007-01-01

    Bifidobacterium breve, een probiotische bacterie, heeft gunstige effecten op zowel allergieen als autoimmuniteit - een afweerreactie op lichaamseigen bestanddelen - bij proefdieren. Probiotica worden in reclameboodschappen ook wel 'goede bacterien' genoemd. Fabrikanten claimen een positief effect

  9. Reliability of the Spanish version of a brief questionnaire on patient satisfaction with gastrointestinal endoscopy Validación de un cuestionario breve sobre satisfacción del paciente en endoscopia digestiva

    Directory of Open Access Journals (Sweden)

    A. Sánchez del Río

    2005-08-01

    Full Text Available Background: there has not been a validated questionnaire available in Spanish to evaluate patient satisfaction with gastrointestinal endoscopy. Our aim was to evaluate the external validity and internal consistency of the Spanish version of a questionnaire on patient satisfaction with gastrointestinal endoscopy elaborated by the American Society for Gastrointestinal Endoscopy. Design: prospective questionnaire validation study. Patients and methods: a total of 485 consecutive patients referred to two different hospitals for endoscopy were interviewed by telephone. Internal consistency was studied using Cronbach's alfa test and corrected item-total correlations (CITC. External validity was determined using a mailed questionnaire completed by 185 patients -correlations between telephone and postal responses were calculated, as well as the correlation with the total score obtained. Results: Cronbach´s alfa was 0.82 and mean CITC was 0.59. Weighted kappa values for the same questionnaire items performed by telephone or mail varied between 0.51 and 0.81. Total score correlation was 0.78. Internal consistency and external validity were not affected by differences in the administration of the questionnaire (mail or by telephone, different interviewers, type of endoscopy, or source of patients. Conclusions: the Spanish version of the ASGE questionnaire on satisfaction with endoscopy is valid, reliable, and reproducible.Objetivos: no se dispone en castellano de un cuestionario validado para la valoración de la satisfacción del paciente en endoscopia digestiva. Evaluar la validez externa y la consistencia interna de la versión en castellano del cuestionario recomendado por la American Society for Gastrointestinal Endoscopy sobre satisfacción en endoscopia. Diseño experimental: estudio de validación de un cuestionario, recogida de datos prospectiva. Pacientes y métodos: cuatrocientos ochenta y cinco pacientes consecutivos remitidos para endoscopia

  10. Dos horrores do inferno na terra: breves notas sobre a construção social de um turismo em conflito com as forças religiosas francesas em um município da Serra Gaúcha

    Directory of Open Access Journals (Sweden)

    Itamar Ferretto Comarú

    2015-12-01

    Full Text Available O estudo analisa o campo turístico e social de Veranópolis/RS. Sobre sua população os sacerdotes praticavam um olhar vigilante, avaliando com atenção as mudanças que se produziam numa sociedade em ebulição, temendo sobremaneira o contato entre visitantes e visitados. Ali, o turismo estilhaçou a ordem estabelecida, perturbou as tradições, abalou os costumes ali existentes, rompeu com a confortável rotina. Entretanto, a semente da natureza enquanto atrativo turístico estava sendo preparada, valorizada inclusive pelos próprios sacerdotes. Assim, quando germina, o turismo local passa a ter um novo caminho, uma manobra astuciosa, que constrói um modelo moderno, até então não existente na cidade, afastando-se dos homens e suas construções culturais, passando a construir uma atividade onde a natureza estaria ao centro de um potente discurso total. Este ato transformador será posto em prática na década de 1950, mas apresentará um claro efeito colateral: o esquecimento da cidade e seus atrativos culturais. Todavia, a localidade não se metamorfoseava somente em seus costumes, que pareciam testados a todo o momento. A materialidade urbana, igualmente, passava por um veloz processo de ruptura entre antigo e moderno, sendo devorada pela aparente necessidade de se constituir uma imagem renovada da cidade, distanciando-se da antiga representação colonial. Ruiria ela também, tempos depois, seguindo as tendências padronizadas, impostas pelo capitalismo imobiliário. Em meio a esse processo, a cidade é renomeada, edificando-se uma nova identidade. Surge a Cidade Veraneio. A soma desses fatores levará ao contemporâneo distanciamento entre turismo e espaço urbano, contrapondo-se à valorização turística da natureza ali existente.

  11. Chrysostom’s exegesis of the Gospel according to St. Matthew

    Directory of Open Access Journals (Sweden)

    Arkadiusz Baron

    2008-06-01

    is the person of Jesus. The prime purpose of Matthew’s Gospel is to reveal the unconditional love of God for each human being. Homilies on Matthew are completely apolitical. Chrysostom never even mentions governors or political situations. Similarly, he does not speak about ecclesiastical canons of councils of Antioch from the fourth century. He is only interested in how to explain the best way to all the listeners the Good News that Jesus has brought on earth. At the end, Fr. Baron gives some examples of Chrysostom’s exegesis: Mt 12: 33-37; 10: 32; 28: 1-3 and Homily on Matthew 85, 3-4.

  12. Breve reflexão sobre autocuidado no planejamento de alta hospitalar pós-Transplante de medula óssea (TMO: relato de caso Breve reflexión sobre lo autocuidado en planeamiento de la alta después del Transplante de Médula Ósea: informe de caso A brief reflection on self-care in hospital discharge planning after a Bone Marrow Transplantation (BMT: a case report

    Directory of Open Access Journals (Sweden)

    Lúcia Marta Giunta da Silva

    2001-01-01

    Full Text Available Este estudo teve por objetivo construir um instrumento que caracterizasse as demandas de autocuidado terapêutico de um indivíduo submetido ao TMO, refletindo sobre as intervenções de enfermagem no planejamento de alta hospitalar na óptica da teoria de autocuidado de Orem. Pelos critérios de inclusão obteve-se um indivíduo pós-TMO alogênico, portador de Leucemia Mielóide Crônica. A análise dos dados possibilitou avaliar os requisitos de autocuidado alterados e as intervenções de enfermagem se inseriram no sistema apoio-educação e no sistema parcialmente compensatório. O instrumento deverá ser refinado, mas pode-se sugerir sua utilização como suporte para a elaboração do planejamento de alta para pacientes de TMO.Este estudio tuvo por objetivo construir un instrumento para caracterizar las demandas del autocuidado terapéutico de un individuo sometido al transplante de médula ósea, reflexionando acerca de las intervenciones de la enfermería en la planeación de la alta hospitalaria, desde la óptica de la teoría del autocuidado de Oren. Por los criterios de inclusión se obtuvo una persona sometida al transplante del médula ósea alogénico, Portador de Leucemia Mieloide Crónica. El análisis de los datos permitió la evaluación de los requisitos de autocuidado alterados y las intervenciones de enfermería se situaron en el sistema apoyo-educación y en el sistema parcialmente compensatorio. El instrumento deberá refinarse, pero se puede sugerir su utilización como apoyo para la elaboración de la planeación del alta hospitalaria, para los pacientes del transplante de médula ósea.The purpose of this work was to construct an instrument that would enable the characterization of therapeutic self-care needs of an individual submitted to bone marrow transplantation (BMT by reflecting on nursing interventions in the planning of hospital discharge based on Orem's self-care theory. The inclusion criteria consisted in the

  13. Cellodextrin utilization by bifidobacterium breve UCC2003.

    Science.gov (United States)

    Pokusaeva, Karina; O'Connell-Motherway, Mary; Zomer, Aldert; Macsharry, John; Fitzgerald, Gerald F; van Sinderen, Douwe

    2011-03-01

    Cellodextrins, the incomplete hydrolysis products from insoluble cellulose, are accessible as a carbon source to certain members of the human gut microbiota, such as Bifidobacterium breve UCC2003. Transcription of the cldEFGC gene cluster of B. breve UCC2003 was shown to be induced upon growth on cellodextrins, implicating this cluster in the metabolism of these sugars. Phenotypic analysis of a B. breve UCC2003::cldE insertion mutant confirmed that the cld gene cluster is exclusively required for cellodextrin utilization by this commensal. Moreover, our results suggest that transcription of the cld cluster is controlled by a LacI-type regulator encoded by cldR, located immediately upstream of cldE. Gel mobility shift assays using purified CldR(His) (produced by the incorporation of a His(12)-encoding sequence into the 3' end of the cldC gene) indicate that the cldEFGC promoter is subject to negative control by CldR(His), which binds to two inverted repeats. Analysis by high-performance anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD) of medium samples obtained during growth of B. breve UCC2003 on a mixture of cellodextrins revealed its ability to utilize cellobiose, cellotriose, cellotetraose, and cellopentaose, with cellotriose apparently representing the preferred substrate. The cldC gene of the cld operon of B. breve UCC2003 is, to the best of our knowledge, the first described bifidobacterial β-glucosidase exhibiting hydrolytic activity toward various cellodextrins.

  14. La mirada del otro. Breve autoficción intelectual

    Directory of Open Access Journals (Sweden)

    Felipe Arocena

    2015-03-01

    Full Text Available Esta breve autobiografía o autoficción intelectual trata de idas y vueltas, de salidas y retornos, y de algunas de las influencias que percibo fueron significativas en mi vida, en mi formación profesional y en mi mirada sobre América Latina. Desde diferentes perspectivas reflexionaré también acerca de cómo esa experiencia académica y vital en Europa y Estados Unidos modeló mi identidad de sociólogo. Ese fue el desafío que me ofreció el editor de la revista y fue el espíritu con el que escribí este texto.

  15. LA MIRADA DEL OTRO. BREVE AUTOFICCIÓN INTELECTUAL

    Directory of Open Access Journals (Sweden)

    Felipe Arocena

    2015-01-01

    Full Text Available Esta breve autobiografía o autoficción intelectual trata de idas y vueltas, de salidas y retornos, y de algunas de las influencias que percibo fueron significativas en mi vida, en mi formación profesional y en mi mirada sobre América Latina. Desde diferentes perspectivas reflexionaré también acerca de cómo esa experiencia académica y vital en Europa y Estados Unidos modeló mi identidad de sociólogo. Ese fue el desafío que me ofreció el editor de la revista y fue el espíritu con el que escribí este texto.

  16. É possível psicanálise breve?

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    Theodor S. Lowenkron

    Full Text Available A psicoterapia psicanalítica de tempo delimitado é aqui definida como um tratamento cujo prazo de duração é ajustado previamente entre o psicoterapeuta (ou analista e o sujeito que busca ajuda para dar conta de um sofrimento psíquico que se manifesta em uma área demarcável da vida do sujeito e cuja origem possa ser atribuída a um conflito inconsciente. A teoria psicanalítica fornece os modelos gerais que fundamentam o processo, e a cura se dá mediante a perlaboração dos conflitos inconscientes, com possíveis repercussões sobre o sujeito como um todo. Teoricamente, essa abordagem terapêutica fundamenta-se nas concepções de Freud, Ferenczi e das pesquisas realizadas na Clínica Tavistock, principalmente, através do experimento de Balint. Essa comunicação foi estimulada não só pelos resultados de pesquisas desenvolvidas pelo autor no campo da psicoterapia psicanalítica de tempo delimitado, mas também pela aprovação, por Resolução do Ministério da Saúde, da cobertura obrigatória pelos planos e seguros privados de saúde para a psicoterapia breve de crise com duração de até 12 sessões. Esse trabalho tem o objetivo de promover o debate em nosso meio sobre o valor da abordagem psicanalítica breve. Para tanto, além da apresentação de uma experiência clínica realizada com uma paciente durante cinco entrevistas psicoterápicas e mais duas entrevistas de follow-up, cerca de seis e 12 meses após o término do tratamento, discutem-se os fundamentos da ampliação do espaço psicanalítico tomando em consideração o posicionamento de Freud e as críticas teóricas e práticas, expostas por Birman, sobre o que pode ser denominado espaço psicanalítico. Nesse trabalho destaca-se ainda a importância de que, ao final da terapia, deve-se fazer uma avaliação dos resultados alcançados e, se o trabalho realizado não for considerado suficiente para a elaboração das tensões conflitivas que deram origem às manifesta

  17. Breve ensayo sobre el devenir del libro moderno

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    Roberto Jalil Milad

    2006-08-01

    Full Text Available El libro ha sufrido modificaciones radicales durante su evolución, que ha acompañado a la evolución intelectual y cultural del hombre. Estas modificaciones han afectado tanto su estructura física como su organización interna. La producción artesanal del libro se modificó definitivamente en el siglo XIV con el advenimiento de la imprenta, transformando en forma decisiva la fabricación y venta de libros en una industria floreciente hasta nuestros días, permitiendo su expansión hacia el hombre común. Posteriormente, en el siglo XVIII, la aparición de la enciclopedia le dio un impulso insospechado, ayudando dinámicamente a modificar la forma en que el hombre accedía al conocimiento. El siglo XX, a través del desarrollo de Internet permitía al libro incorporarse a una matriz activa del conocimiento. Las características de Internet, como protovehículo de inteligencia artificial, plantea la disyuntiva de su origen desde una dimensión casual o causal, en una comparación inevitable con los orígenes mismos del lenguaje.

  18. BREVES CONSIDERACIONES SOBRE EL BOXEO Y EL DERECHO

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    Luis Ariel Salanueva Brito

    2016-07-01

    Full Text Available A través de una reflexión, el autor determina analogías entre el Derecho y el boxeo, donde da fe de las coincidencias que en cuestión de habilidades se presentan en ambas tareas, en ambos compromisos; haciendo de la abogacía, una profesión naturalmente relacionada con el pugilismo.

  19. BREVES CONSIDERACIONES SOBRE EL BOXEO Y EL DERECHO

    OpenAIRE

    Brito, Luis Ariel Salanueva; Escuela Libre de Derecho (ELD)

    2016-01-01

    A través de una reflexión, el autor determina analogías entre el Derecho y el boxeo, donde da fe de las coincidencias que en cuestión de habilidades se presentan en ambas tareas, en ambos compromisos; haciendo de la abogacía, una profesión naturalmente relacionada con el pugilismo.

  20. Terapia breve em hospital fechado

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    Paulo Cesar Sandler

    1978-09-01

    Full Text Available O presente estudo cuida de pacientes "difíceis" da clínica psiquiátrica e psicoterápica: os pacientes que apresentam o fenômeno da atuação, cuja aproximação terapêutica tem se demonstrado problemática, mesmo com o advento das modernas técnicas de psicoterapia e das comunidades terapêuticas. Os diagnósticos psiquiátricos destes pacientes variam: são neuróticos, apresentam transtornos de pesonalidade, são psicóticos e quase sempre com graves doenças secundárias: alcoolismo e toxicomania (Códigos Classificação Internacional de Doenças 295 a 299, 300. 301, 303 e 304. Propõe-se, à luz da compreensão psicanalítica destas pessoas, um tratamento psicoterápico breve, focal, com o auxílio de uma hospitalização em regime fechado. Para a avaliação dos resultados do método psicoterápico diádico, criaram-se critérios de cura coerentes com a proposta terapêutica, e equiparam-se estes critérios clínicos a valores numéricos. Utilizando 36 pacientes internados, verificou-se até que ponto eles alcançavam estes critérios. Cada paciente recebeu um escore - valor numérico - que expressou o seu desempenho durante o tratamento em regime de internação, e sua conduta pós-alta hospitalar, durante um seguimento médio de 21 meses. Estes 36 pacientes puderam ser divididos em dois grupos: os de evolução favorável (obtivemos 36,1% e os de evolução desfavorável. Por meio de uma amostra sistemática composta por 41 pacientes que não foram submetidos ao método, internados no mesmo período de tempo, no mesmo hospital, e pareados com nossa casuística, fêz-se uma comparação estatística entre os percentis obtidos nas evoluções dos dois grupos. O valor do teste estatístico, o chi quadrado, não foi significante. O exame das proporções correspondentes a cada patologia em separado sugeriu fortemente que o tratamento estaria indicado nas neuroses e nas psicoses, mas que seria realmente ineficaz nos transtornos de

  1. Immune effects of the probiotic Bifidobacterium breve

    OpenAIRE

    Ezendam J; Loveren H van; TOX

    2007-01-01

    Bifidobacterium breve, een probiotische bacterie, heeft gunstige effecten op zowel allergieen als autoimmuniteit - een afweerreactie op lichaamseigen bestanddelen - bij proefdieren. Probiotica worden in reclameboodschappen ook wel 'goede bacterien' genoemd. Fabrikanten claimen een positief effect van probiotica op darmflora, weerstand en preventie van allergieen. De meeste van deze effecten zijn echter niet wetenschappelijk onderbouwd. Het is bekend dat de effecten van probiotica afhangen van...

  2. Comparative genomics of the Bifidobacterium breve taxon.

    Science.gov (United States)

    Bottacini, Francesca; O'Connell Motherway, Mary; Kuczynski, Justin; O'Connell, Kerry Joan; Serafini, Fausta; Duranti, Sabrina; Milani, Christian; Turroni, Francesca; Lugli, Gabriele Andrea; Zomer, Aldert; Zhurina, Daria; Riedel, Christian; Ventura, Marco; van Sinderen, Douwe

    2014-03-01

    Bifidobacteria are commonly found as part of the microbiota of the gastrointestinal tract (GIT) of a broad range of hosts, where their presence is positively correlated with the host's health status. In this study, we assessed the genomes of thirteen representatives of Bifidobacterium breve, which is not only a frequently encountered component of the (adult and infant) human gut microbiota, but can also be isolated from human milk and vagina. In silico analysis of genome sequences from thirteen B. breve strains isolated from different environments (infant and adult faeces, human milk, human vagina) shows that the genetic variability of this species principally consists of hypothetical genes and mobile elements, but, interestingly, also genes correlated with the adaptation to host environment and gut colonization. These latter genes specify the biosynthetic machinery for sortase-dependent pili and exopolysaccharide production, as well as genes that provide protection against invasion of foreign DNA (i.e. CRISPR loci and restriction/modification systems), and genes that encode enzymes responsible for carbohydrate fermentation. Gene-trait matching analysis showed clear correlations between known metabolic capabilities and characterized genes, and it also allowed the identification of a gene cluster involved in the utilization of the alcohol-sugar sorbitol. Genome analysis of thirteen representatives of the B. breve species revealed that the deduced pan-genome exhibits an essentially close trend. For this reason our analyses suggest that this number of B. breve representatives is sufficient to fully describe the pan-genome of this species. Comparative genomics also facilitated the genetic explanation for differential carbon source utilization phenotypes previously observed in different strains of B. breve.

  3. Wordstar professional : breve guida di consultazione

    OpenAIRE

    Biagioni, Stefania

    1990-01-01

    La presente nota ? una breve guida all'uso di WordStar Professional 4.0, utile per una rapida consultazione. Non intende esaurire l'argomento e neppure riportare tutto quello che pu? essere trovato nei manuali citati nella bibliografia presente nel testo. I comandi sono trattati secondo il raggruppamento dei MENU guida che WordStar offre agli utenti, mentre alcune delle funzioni pi? complesse sono spiegate pi? in dettaglio unitamente ad alcuni esempi.

  4. ENTRE LIBERDADE E REPRESENTATIVIDADE: BREVES CONSIDERAÇÕES SOBRE A LEI N. 13.467/2017, A PROPOSTA DE AUTONOMIA DO NEGOCIADO SOBRE O LEGISLADO E O PAPEL DO JUDICIÁRIO NA ANÁLISE DOS ACORDOS E CONVENÇÕES COLETIVAS

    OpenAIRE

    Bento da Silva, Lays Caceres

    2017-01-01

    A recém-publicada Lei n. 13.467, de 13 de julho de 2017, também chamada de reforma trabalhista, tem por objetivo promover alterações na Consolidação das Leis do Trabalho (Decreto-Lei no 5.452/43) e na Lei no 6.019/74, que dispõe sobre o sistema de trabalho temporário. O referido texto, que promete maior adequação à realidade econômica brasileira e redução das matérias levadas ao judiciário, vem sofrendo críticas dos mais diversos setores, incluindo os sindicatos, o Ministéri...

  5. The explanation of muwālāt in Zuhaily’s exegesis “al Tafsir al munir”

    Directory of Open Access Journals (Sweden)

    Iman Kanani

    2016-03-01

    Full Text Available One of the crucial contemporary issues of Islamic thought that has brought complicated situation in Muslims states, on the one hand, and between Muslims and non-Muslims on the other hand, is the issue of muwālāt. The literal understanding of muwālāt has caused profound problems in human societies, especially in the multi-religion countries. This research clarifies the meaning of loyalty in the Qur’ān based on al-Zuḥayli’s exegesis by using analytical -inductive method. The present study also elucidates Al-Zuḥayli’s point of view towards spiritual muwālāt and humane social muwālāt in the Quran.Hence, the study reveals fruitful findings and output, indicating that al-Zuḥayli separated muwālāt to three categories; prohibited muwālāt, muwālāt that causes infidelity, and muwālāt which means nice cohabitation with others. Also, the study explores that al-Zuḥayli believes that the principle in relations with Non-Muslims is based on peace. Furtheremore, al-Zuḥayli pointed out that muwālāt is permissible when it benefits Muslim society and Non- Muslim are not fighting with or betraying Muslims. Also the study presents that al-Zuḥayli has distinguished between muwālāt that means monotheism and worshiping God, and other types of muwālāt.

  6. 'Trial Exegesis': Methods for Synthesizing Clinical and Patient Reported Outcome (PRO Data in Trials to Inform Clinical Practice. A Systematic Review.

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    Angus G K McNair

    Full Text Available The CONSORT extension for patient reported outcomes (PROs aims to improve reporting, but guidance on the optimal integration with clinical data is lacking. This study examines in detail the reporting of PROs and clinical data from randomized controlled trials (RCTs in gastro-intestinal cancer to inform design and reporting of combined PRO and clinical data from trials to improve the 'take home' message for clinicians to use in practice.The case study was undertaken in gastro-intestinal cancer trials. Well-conducted RCTs reporting PROs with validated instruments were identified and categorized into those combining PRO and clinical data in a single paper, or those separating data into linked primary and supplemental papers. Qualitative methods were developed to examine reporting of the critical interpretation of the trial results (trial exegesis in the papers in relation of the PRO and clinical outcomes and applied to each publication category. Results were used to inform recommendations for practice.From 1917 screened abstracts, 49 high quality RCTs were identified reported in 36 combined and 15 linked primary and supplemental papers. In-depth analysis of manuscript text identified three categories for understanding trial exegesis: where authors reported a "detailed", "general", or absent PRO rationale and integrated interpretation of clinical and PRO results. A total of 11 (30% and 6 (16% combined papers reported "detailed" PRO rationale and integrated interpretation of results although only 2 (14% and 1 (7% primary papers achieved the same standard respectively. Supplemental papers provide better information with 11 (73% and 3 (20% achieving "detailed" rationale and integrated interpretation of results. Supplemental papers, however, were published a median of 20 months after the primary RCT data in lower impact factor journals (median 16.8 versus 5.2.It is recommended that single papers, with detailed PRO rationale and integrated PRO and

  7. Impacto de una intervención breve basada en mindfulness en niños: un estudio piloto

    OpenAIRE

    García Rubio, Carlos; Luna Jarillo, Teodoro; Castillo Gualda, Ruth; Rodríguez Carvajal, Raquel

    2016-01-01

    En la última década el interés por los efectos de la práctica de mindfulness ha sido exponencial. Sin embargo, todavía es escasa la investigación sobre sus efectos en el contexto escolar. Este estudio piloto examinó la efectividad de una intervención breve basada en mindfulness en estudiantes de primaria. Se diseñó un estudio pre-post aleatorizado a nivel de clase con un grupo control. Se investigaron sus efectos sobre distintos síntomas internalizantes y externalizantes, las habilidades de m...

  8. 王弼《老子注》的詮釋辨證/ Interpretative Dialectics of Wang Bi’s Exegesis of Lao-Tzu

    Directory of Open Access Journals (Sweden)

    Jung-Tao TSAI

    2012-09-01

    Wang Bi, the most prominent scholar of metaphysics of the Wei and Jin Dynasties, was an unprecedented genius throughout the history of Chinese philosophy. In this article, the author examines the brilliant contributions made by Lin Li Zhen, Yu Dun Kang and Rudolf G. Wagner, who are noticed for their research of Exegesis of Lao-Tzu. It is a synthesis of the directions of investigation, approaches, and significant findings they have come up with. Among the three interpretations, Lin focuses on the explanation of Wang’s metaphysic thinking while Yu emphasizes Wang’s secular concerns by proposing the “philosophy of thoroughness” and political holism. As for Wagner, he approaches this work with the views of linguistic philosophy and suggests refined stylistics as interpretative strategy, which functions to enrich the textual contents.

  9. Avaliação de resultados da capacitação via estágios pós-doutorais: breves notas sobre a produção científica em periódicos Evaluación de resultados de la capacitación por medio de etapas posdoctorales: breves notas sobre la producción científica en revistas Evaluation of Outputs of Post-doctoral Research: brief notes about the scientific production in journals

    Directory of Open Access Journals (Sweden)

    Pedro Marcos Roma de Castro

    2012-03-01

    Full Text Available A pesquisa teve como objetivo avaliar a influência do estágio pós-doutoral sobre a produção científica, notadamente das hard sciences de docentes atuantes em programas de pós-graduação da Universidade de São Paulo (USP. O estudo busca a mensuração de variações da produção registrada em periódicos científicos em um panorama ex ante e ex post e encontra-se circunscrito nessas quatro grandes áreas do conhecimento: Biológicas, Engenharias, Exatas e da Terra e Saúde. O pós-doutorado pode ser visualizado como um local privilegiado para a complementaridade da capacitação docente enquanto forma de atualização e/ou reciclagem profissional, com a lógica embutida e a expectativa de que haja benefícios, também, para o programa de pós-graduação de origem. Como toda atividade de pesquisa não pode ficar implícita, as atividades de pesquisa do pós-doutorado, também, necessitam ser tornadas públicas via conhecimento externalizado que se traduz no conhecimento cristalizado sob a forma de publicações científicas que são compartilhadas pela comunidade acadêmica. Com uma população de 86 doutores que realizaram pósdoutorado até junho de 2005, os dados levantados com o confronto da produção em Ciência e Tecnologia (C&T, antes e depois do treinamento pós-doutoral apontam para um comportamento de variação da produtividade em mídia qualificada cujo resultado demonstra uma "homogeneização" relativa entre publicações registradas em periódicos Qualis 'B' e 'C', nacionais e/ou internacionais, mas sugere haver diferenças no que tange a uma influência sobre o aumento de publicações em revistas Internacionais Qualis 'A' possuindo influência positiva mais visível sobre essa produção para estágios realizados no Exterior, nas grandes áreas do conhecimento investigadas.Esta investigación tiene por objeto evaluar la influencia de la etapa del posdoctorado sobre la producción científica, específicamente de las

  10. Cellodextrin Utilization by Bifidobacterium breve UCC2003▿ †

    Science.gov (United States)

    Pokusaeva, Karina; O'Connell-Motherway, Mary; Zomer, Aldert; MacSharry, John; Fitzgerald, Gerald F.; van Sinderen, Douwe

    2011-01-01

    Cellodextrins, the incomplete hydrolysis products from insoluble cellulose, are accessible as a carbon source to certain members of the human gut microbiota, such as Bifidobacterium breve UCC2003. Transcription of the cldEFGC gene cluster of B. breve UCC2003 was shown to be induced upon growth on cellodextrins, implicating this cluster in the metabolism of these sugars. Phenotypic analysis of a B. breve UCC2003::cldE insertion mutant confirmed that the cld gene cluster is exclusively required for cellodextrin utilization by this commensal. Moreover, our results suggest that transcription of the cld cluster is controlled by a LacI-type regulator encoded by cldR, located immediately upstream of cldE. Gel mobility shift assays using purified CldRHis (produced by the incorporation of a His12-encoding sequence into the 3′ end of the cldC gene) indicate that the cldEFGC promoter is subject to negative control by CldRHis, which binds to two inverted repeats. Analysis by high-performance anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD) of medium samples obtained during growth of B. breve UCC2003 on a mixture of cellodextrins revealed its ability to utilize cellobiose, cellotriose, cellotetraose, and cellopentaose, with cellotriose apparently representing the preferred substrate. The cldC gene of the cld operon of B. breve UCC2003 is, to the best of our knowledge, the first described bifidobacterial β-glucosidase exhibiting hydrolytic activity toward various cellodextrins. PMID:21216899

  11. Voz sobre frame relay

    OpenAIRE

    D´Elia, Gabriel Anibal

    2000-01-01

    Esta tesis trata el tema de VOFR, desde la digitalización de la voz hasta su transmisión a través de dicha red, así también como la comparación con otros medios de transporte como VOIP. Dada las características del protocolo frame relay y su disponibilidad se eligió como el medio más apropiado para la transmisión de voz y datos en forma integrada sobre una misma red. El trabajo comienza con una breve explicación de la voz, su digitalización y forma actual de transmisión a través de una red di...

  12. Discovery of a conjugative megaplasmid in Bifidobacterium breve.

    Science.gov (United States)

    Bottacini, Francesca; O'Connell Motherway, Mary; Casey, Eoghan; McDonnell, Brian; Mahony, Jennifer; Ventura, Marco; van Sinderen, Douwe

    2015-01-01

    Bifidobacterium breve is a common and sometimes very abundant inhabitant of the human gut. Genome sequencing of B. breve JCM 7017 revealed the presence of an extrachromosomal element, designated pMP7017 consisting of >190 kb, thus representing the first reported bifidobacterial megaplasmid. In silico characterization of this element revealed several genomic features supporting a stable establishment of the megaplasmid in its host, illustrated by predicted CRISPR-Cas functions that are known to protect the host against intrusion of foreign DNA. Interestingly, pMP7017 is also predicted to encode a conjugative DNA transfer apparatus and, consistent with this notion, we demonstrate here the conjugal transfer of pMP7017 to representative strains of B. breve and B. longum subsp. longum. We also demonstrate the presence of a megaplasmid with homology to pMP7017 in three B. longum subsp. longum strains. Copyright © 2015, American Society for Microbiology. All Rights Reserved.

  13. Discovery of a Conjugative Megaplasmid in Bifidobacterium breve

    Science.gov (United States)

    Bottacini, Francesca; O'Connell Motherway, Mary; Casey, Eoghan; McDonnell, Brian; Mahony, Jennifer; Ventura, Marco

    2014-01-01

    Bifidobacterium breve is a common and sometimes very abundant inhabitant of the human gut. Genome sequencing of B. breve JCM 7017 revealed the presence of an extrachromosomal element, designated pMP7017 consisting of >190 kb, thus representing the first reported bifidobacterial megaplasmid. In silico characterization of this element revealed several genomic features supporting a stable establishment of the megaplasmid in its host, illustrated by predicted CRISPR-Cas functions that are known to protect the host against intrusion of foreign DNA. Interestingly, pMP7017 is also predicted to encode a conjugative DNA transfer apparatus and, consistent with this notion, we demonstrate here the conjugal transfer of pMP7017 to representative strains of B. breve and B. longum subsp. longum. We also demonstrate the presence of a megaplasmid with homology to pMP7017 in three B. longum subsp. longum strains. PMID:25326305

  14. VARIABLE BREVETOXIN PRODUCTION IN GYMNODINIUM BREVE ATTRIBUTABLE TO GROWTH CONDITIONS AND STRAIN DIFFERENCES

    Science.gov (United States)

    Blooms of the dinoflagellate Gymnodinium breve can produce sufficient concentrations of brevetoxin to negatively impact coastal ecosystems, human health, and local economies. Assessment of risks associated with G. breve blooms and subsequent brevetoxin exposure requires better u...

  15. Complete genome sequence of Bifidobacterium breve CECT 7263, a strain isolated from human milk.

    Science.gov (United States)

    Jiménez, Esther; Villar-Tajadura, M Antonia; Marín, María; Fontecha, Javier; Requena, Teresa; Arroyo, Rebeca; Fernández, Leónides; Rodríguez, Juan M

    2012-07-01

    Bifidobacterium breve is an actinobacterium frequently isolated from colonic microbiota of breastfeeding babies. Here, we report the complete and annotated genome sequence of a B. breve strain isolated from human milk, B. breve CECT 7263. The genome sequence will provide new insights into the biology of this potential probiotic organism and will allow the characterization of genes related to beneficial properties.

  16. Macrolide Resistance Mediated by a Bifidobacterium breve Membrane Protein

    OpenAIRE

    Margolles, Abelardo; Moreno, José Antonio; van Sinderen, Douwe; de los Reyes-Gavilán, Clara G.

    2005-01-01

    A gene coding for a hypothetical membrane protein from Bifidobacterium breve was expressed in Lactococcus lactis. Immunoblotting demonstrated that this protein is located in the membrane. Phenotypical changes in sensitivity towards 21 antibiotics were determined. The membrane protein-expressing cells showed higher levels of resistance to several macrolides.

  17. Entrevista breve a Samuel Amaral (CONICET / UNTREF)

    OpenAIRE

    Cuestiones de Sociología

    2013-01-01

    A tres décadas de la recuperación democrática en la Argentina y de la cristalización de otros procesos de transición a la democracia en América Latina, este número especial de la Revista Cuestiones de Sociología está orientado a reflexionar críticamente sobre diferentes debates y conflictos acaecidos a lo largo de estos treinta años en nuestro país. En tal sentido, formulamos las siguientes preguntas a un conjunto de sociólogos destacados de nuestros medio. Departamento de Sociología...

  18. Trayectorias (sobre ruedas): un ensayo visual sobre los carritos de supermercado en la ciudad

    OpenAIRE

    Cruz, Edgar Gómez

    2014-01-01

    El siguiente trabajo es un breve bosquejo de las posibilidades del concepto de trabajo “trayectoria”. Una trayectoria no es sólo, en el sentido literal del término, el trazo del movimiento sobre un camino, sino que busca establecer una reflexión sobre las posibilidades de recolección de registros visuales/digitales/móviles para la investigación etnográfica desde la aleatoriedad del movimiento por la ciudad. El concepto de trayectoria busca dialogar con el de “flâneur” de De Certeau y Benjamin...

  19. Outro lado da fronteira agrícola: breve história sobre a origem e declínio da agricultura autóctone no cerrado The other side of agricultural frontier: a brief history on origin and decline of cerrado's ndian agriculture

    Directory of Open Access Journals (Sweden)

    Rafael Feltran-Barbieri

    2010-12-01

    Full Text Available A tese de frederic Jakson turner, para quem a conquista das fronteiras forjou o caráter americano, convergindo à ascensão da vida privada com o desenvolvimento da democracia e economia, não vingou na América latina porque aqui a realidade mostrou o outro lado da fronteira: um espaço para onde os conflitos sociais dos centros dinâmicos eram expurgados e exacerbados, como muito bem descreveu otávio velho. nesse processo recorrente os primeiros confrontos não ocorriam entre a população migrante e os trabalhadores contratados pelo latifundiário ausente, mas entre todos contra os indígenas. muito se tem escrito sobre esses conflitos, mas o enfoque é sempre o daquele mais drástico e urgente: o genocídio. Pouco, entretanto, se discorre sobre o declínio subsidiário, ou seja, o dos processos agrícolas. Isso é ainda mais verdadeiro quando se trata do Domínio do Cerrado. o presente artigo pretende oferecer uma brevíssima história da origem e declínio da agricultura dos índios no Cerrado.Turner's thesis that frontier had forged convergence between democracy and individual economic ascension did not inspire Latin American critical studies because here the reality showed the other side of the frontier: a space where the social conflicts of the dynamic centers were purged and exacerbated. In this process, the first conflicts did not occur among the migrant population and workers hired by the absent landowner, but among everyone else against the Indians. Much has been discussed about it but very few have focused on the other side of frontiers: the indigenous agriculture. This is clearer when one considers the Cerrado Region. In the past 10 years only 5 articles, including national and international journals, analyzed the Indian Agriculture History and Practices in Cerrado. The goal of this paper is to offer a brief history of Indians agrarian systems, from their origin to decline, in Brazilian Cerrados.

  20. Glycosulfatase-Encoding Gene Cluster in Bifidobacterium breve UCC2003.

    Science.gov (United States)

    Egan, Muireann; Jiang, Hao; O'Connell Motherway, Mary; Oscarson, Stefan; van Sinderen, Douwe

    2016-11-15

    Bifidobacteria constitute a specific group of commensal bacteria typically found in the gastrointestinal tract (GIT) of humans and other mammals. Bifidobacterium breve strains are numerically prevalent among the gut microbiota of many healthy breastfed infants. In the present study, we investigated glycosulfatase activity in a bacterial isolate from a nursling stool sample, B. breve UCC2003. Two putative sulfatases were identified on the genome of B. breve UCC2003. The sulfated monosaccharide N-acetylglucosamine-6-sulfate (GlcNAc-6-S) was shown to support the growth of B. breve UCC2003, while N-acetylglucosamine-3-sulfate, N-acetylgalactosamine-3-sulfate, and N-acetylgalactosamine-6-sulfate did not support appreciable growth. By using a combination of transcriptomic and functional genomic approaches, a gene cluster designated ats2 was shown to be specifically required for GlcNAc-6-S metabolism. Transcription of the ats2 cluster is regulated by a repressor open reading frame kinase (ROK) family transcriptional repressor. This study represents the first description of glycosulfatase activity within the Bifidobacterium genus. Bifidobacteria are saccharolytic organisms naturally found in the digestive tract of mammals and insects. Bifidobacterium breve strains utilize a variety of plant- and host-derived carbohydrates that allow them to be present as prominent members of the infant gut microbiota as well as being present in the gastrointestinal tract of adults. In this study, we introduce a previously unexplored area of carbohydrate metabolism in bifidobacteria, namely, the metabolism of sulfated carbohydrates. B. breve UCC2003 was shown to metabolize N-acetylglucosamine-6-sulfate (GlcNAc-6-S) through one of two sulfatase-encoding gene clusters identified on its genome. GlcNAc-6-S can be found in terminal or branched positions of mucin oligosaccharides, the glycoprotein component of the mucous layer that covers the digestive tract. The results of this study provide

  1. Contrasting effects of Bifidobacterium breve NCIMB 702258 and Bifidobacterium breve DPC 6330 on the composition of murine brain fatty acids and gut microbiota.

    Science.gov (United States)

    Wall, Rebecca; Marques, Tatiana M; O'Sullivan, Orla; Ross, R Paul; Shanahan, Fergus; Quigley, Eamonn M; Dinan, Timothy G; Kiely, Barry; Fitzgerald, Gerald F; Cotter, Paul D; Fouhy, Fiona; Stanton, Catherine

    2012-05-01

    We previously showed that microbial metabolism in the gut influences the composition of bioactive fatty acids in host adipose tissue. This study compared the effect of dietary supplementation for 8 wk with human-derived Bifidobacterium breve strains on fat distribution and composition and the composition of the gut microbiota in mice. C57BL/6 mice (n = 8 per group) received B. breve DPC 6330 or B. breve NCIMB 702258 (10(9) microorganisms) daily for 8 wk or no supplement (controls). Tissue fatty acid composition was assessed by gas-liquid chromatography while 16S rRNA pyrosequencing was used to investigate microbiota composition. Visceral fat mass and brain stearic acid, arachidonic acid, and DHA were higher in mice supplemented with B. breve NCIMB 702258 than in mice in the other 2 groups (P breve DPC 6330 and B. breve NCIMB 702258 supplementation resulted in higher propionate concentrations in the cecum than did no supplementation (P breve DPC 6330 than in mice supplemented with B. breve NCIMB 702258 and unsupplemented controls, respectively. The response of fatty acid metabolism to administration of bifidobacteria is strain-dependent, and strain-strain differences are important factors that influence modulation of the gut microbial community by ingested microorganisms.

  2. Metabolism of sialic acid by Bifidobacterium breve UCC2003.

    Science.gov (United States)

    Egan, Muireann; O'Connell Motherway, Mary; Ventura, Marco; van Sinderen, Douwe

    2014-07-01

    Bifidobacteria constitute a specific group of commensal bacteria that inhabit the gastrointestinal tracts of humans and other mammals. Bifidobacterium breve UCC2003 has previously been shown to utilize several plant-derived carbohydrates that include cellodextrins, starch, and galactan. In the present study, we investigated the ability of this strain to utilize the mucin- and human milk oligosaccharide (HMO)-derived carbohydrate sialic acid. Using a combination of transcriptomic and functional genomic approaches, we identified a gene cluster dedicated to the uptake and metabolism of sialic acid. Furthermore, we demonstrate that B. breve UCC2003 can cross feed on sialic acid derived from the metabolism of 3'-sialyllactose, an abundant HMO, by another infant gut bifidobacterial strain, Bifidobacterium bifidum PRL2010. Copyright © 2014, American Society for Microbiology. All Rights Reserved.

  3. Metabolism of Sialic Acid by Bifidobacterium breve UCC2003

    Science.gov (United States)

    Egan, Muireann; O'Connell Motherway, Mary; Ventura, Marco

    2014-01-01

    Bifidobacteria constitute a specific group of commensal bacteria that inhabit the gastrointestinal tracts of humans and other mammals. Bifidobacterium breve UCC2003 has previously been shown to utilize several plant-derived carbohydrates that include cellodextrins, starch, and galactan. In the present study, we investigated the ability of this strain to utilize the mucin- and human milk oligosaccharide (HMO)-derived carbohydrate sialic acid. Using a combination of transcriptomic and functional genomic approaches, we identified a gene cluster dedicated to the uptake and metabolism of sialic acid. Furthermore, we demonstrate that B. breve UCC2003 can cross feed on sialic acid derived from the metabolism of 3′-sialyllactose, an abundant HMO, by another infant gut bifidobacterial strain, Bifidobacterium bifidum PRL2010. PMID:24814790

  4. Transposon mutagenesis in Bifidobacterium breve: construction and characterization of a Tn5 transposon mutant library for Bifidobacterium breve UCC2003.

    Science.gov (United States)

    Ruiz, Lorena; Motherway, Mary O'Connell; Lanigan, Noreen; van Sinderen, Douwe

    2013-01-01

    Bifidobacteria are claimed to contribute positively to human health through a range of beneficial or probiotic activities, including amelioration of gastrointestinal and metabolic disorders, and therefore this particular group of gastrointestinal commensals has enjoyed increasing industrial and scientific attention in recent years. However, the molecular mechanisms underlying these probiotic mechanisms are still largely unknown, mainly due to the fact that molecular tools for bifidobacteria are rather poorly developed, with many strains lacking genetic accessibility. In this work, we describe the generation of transposon insertion mutants in two bifidobacterial strains, B. breve UCC2003 and B. breve NCFB2258. We also report the creation of the first transposon mutant library in a bifidobacterial strain, employing B. breve UCC2003 and a Tn5-based transposome strategy. The library was found to be composed of clones containing single transposon insertions which appear to be randomly distributed along the genome. The usefulness of the library to perform phenotypic screenings was confirmed through identification and analysis of mutants defective in D-galactose, D-lactose or pullulan utilization abilities.

  5. Transposon mutagenesis in Bifidobacterium breve: construction and characterization of a Tn5 transposon mutant library for Bifidobacterium breve UCC2003.

    Directory of Open Access Journals (Sweden)

    Lorena Ruiz

    Full Text Available Bifidobacteria are claimed to contribute positively to human health through a range of beneficial or probiotic activities, including amelioration of gastrointestinal and metabolic disorders, and therefore this particular group of gastrointestinal commensals has enjoyed increasing industrial and scientific attention in recent years. However, the molecular mechanisms underlying these probiotic mechanisms are still largely unknown, mainly due to the fact that molecular tools for bifidobacteria are rather poorly developed, with many strains lacking genetic accessibility. In this work, we describe the generation of transposon insertion mutants in two bifidobacterial strains, B. breve UCC2003 and B. breve NCFB2258. We also report the creation of the first transposon mutant library in a bifidobacterial strain, employing B. breve UCC2003 and a Tn5-based transposome strategy. The library was found to be composed of clones containing single transposon insertions which appear to be randomly distributed along the genome. The usefulness of the library to perform phenotypic screenings was confirmed through identification and analysis of mutants defective in D-galactose, D-lactose or pullulan utilization abilities.

  6. Complete genome sequence of Bifidobacterium breve CECT 7263, a strain isolated from human milk

    OpenAIRE

    Jiménez, Esther; Villar-Tajadura, M. Antonia; Marín, María; Fontecha, F. Javier; Requena, Teresa; Arroyo, Rebeca; Fernández, Leónides; Rodríguez, Juan M.

    2012-01-01

    Bifidobacterium breve is an actinobacterium frequently isolated from colonic microbiota of breastfeeding babies. Here, we report the complete and annotated genome sequence of a B. breve strain isolated from human milk, B. breve CECT 7263. The genome sequence will provide new insights into the biology of this potential probiotic organism and will allow the characterization of genes related to beneficial properties. © 2012, American Society for Microbiology.

  7. Modulation of Rat Cecal Microbiota by Administration of Raffinose and Encapsulated Bifidobacterium breve

    OpenAIRE

    Dinoto, Achmad; Suksomcheep, Akarat; Ishizuka, Satoshi; Kimura, Hanae; Hanada, Satoshi; Kamagata, Yoichi; Asano, Kozo; Tomita, Fusao; Yokota, Atsushi

    2006-01-01

    To investigate the effects of administration of raffinose and encapsulated Bifidobacterium breve JCM 1192T cells on the rat cecal microbiota, in a preclinical synbiotic study groups of male WKAH/Hkm Slc rats were fed for 3 weeks with four different test diets: basal diet (group BD), basal diet supplemented with raffinose (group RAF), basal diet supplemented with encapsulated B. breve (group CB), and basal diet supplemented with both raffinose and encapsulated B. breve (group RCB). The bacteri...

  8. ¿Los Mejores Perfumes Vienen en Envases Pequeños?: Potencial de las Intervenciones Breves en el Contexto Educativo/ The Best Perfumes Come in Small Packages? The Potential of Brief Interventions in the Educational Context/ São nos Menores Frascos que se Encontram os Melhores Perfumes? Potencial das Intervenções Breves no Contexto Educativo

    Directory of Open Access Journals (Sweden)

    MADELEINE BARRERA

    2015-10-01

    Full Text Available Recientemente se han desarrollado intervenciones escolares breves basadas en teorías motivacionales y de psicología social. Estas intervenciones, algunas de solamente 15 minutos, han obtenido resultados positivos a largo plazo sobre dominios como el desempeño académico y la convivencia escolar. Este artículo hace una revisión de sus objetivos, características, fundamentos teóricos, evaluaciones y condiciones necesarias para su diseño e implementación. En general, el objetivo de estas intervenciones breves es transformar creencias, pensamientos o sentimientos de un segmento particular de la población estudiantil, considerado en riesgo. Además, buscan romper círculos viciosos al intervenir en momentos específicos de procesos recursivos. Finalmente, esta revisión analiza sus alcances, implicaciones y limitaciones, y sugiere futuras investigaciones.

  9. Curso CC 2010: Sobre proyectos de cultura científica

    OpenAIRE

    Ferrando González, Laura; Martínez Cáceres, Rafael

    2010-01-01

    Breves apuntes sobre los proyectos de cultura científica, su estructura y elementos, elaborados en el marco del curso de formación "Cultura científica: divulgación y comunicación de la ciencia", organizado por la Vicepresidenta Adjunta de Organización y Cultura Científica para personal del CSIC.

  10. Probiotic Bifidobacterium breve induces IL-10-producing Tr1 cells in the colon.

    Directory of Open Access Journals (Sweden)

    Seong Gyu Jeon

    Full Text Available Specific intestinal microbiota has been shown to induce Foxp3(+ regulatory T cell development. However, it remains unclear how development of another regulatory T cell subset, Tr1 cells, is regulated in the intestine. Here, we analyzed the role of two probiotic strains of intestinal bacteria, Lactobacillus casei and Bifidobacterium breve in T cell development in the intestine. B. breve, but not L. casei, induced development of IL-10-producing Tr1 cells that express cMaf, IL-21, and Ahr in the large intestine. Intestinal CD103(+ dendritic cells (DCs mediated B. breve-induced development of IL-10-producing T cells. CD103(+ DCs from Il10(-/-, Tlr2(-/-, and Myd88(-/- mice showed defective B. breve-induced Tr1 cell development. B. breve-treated CD103(+ DCs failed to induce IL-10 production from co-cultured Il27ra(-/- T cells. B. breve treatment of Tlr2(-/- mice did not increase IL-10-producing T cells in the colonic lamina propria. Thus, B. breve activates intestinal CD103(+ DCs to produce IL-10 and IL-27 via the TLR2/MyD88 pathway thereby inducing IL-10-producing Tr1 cells in the large intestine. Oral B. breve administration ameliorated colitis in immunocompromised mice given naïve CD4(+ T cells from wild-type mice, but not Il10(-/- mice. These findings demonstrate that B. breve prevents intestinal inflammation through the induction of intestinal IL-10-producing Tr1 cells.

  11. Bacteremia induced by Bifidobacterium breve in a newborn with cloacal exstrophy.

    Science.gov (United States)

    Sato, Shinichi; Uchida, Toshihiko; Kuwana, Shota; Sasaki, Kazuto; Watanabe, Tatsuya; Saito, Junko; Kawaji, Takahiro

    2016-11-01

    Bifidobacterium breve is an effective probiotic agent used in the field of neonatology. Although B. breve has been considered safe, a case of B. breve bacteremia has been reported. The pathogenic mechanism underlying the bacteremia is unknown. Herein, we report a second case of B. breve bacteremia that developed in a neonate with multiple abdominal organ anomalies. Following surgical repair immediately after birth, B. breve treatment was started. After 1 week, the infant developed B. breve bacteremia following the onset of adhesive ileus. The bacteremia was thought to have been associated with an intestinal obstruction. A pediatric culture bottle is theoretically unsuitable for incubating B. breve because B. breve is an obligate anaerobic bacterium. It was, however, cultured from pediatric culture bottles in the present case, suggesting that pediatric culture bottles may be useful for procuring B. breve and for determining antimicrobial susceptibility for screening purposes in neonatal patients. © 2016 Japan Pediatric Society.

  12. Probiotic Bifidobacterium breve induces IL-10-producing Tr1 cells in the colon.

    Science.gov (United States)

    Jeon, Seong Gyu; Kayama, Hisako; Ueda, Yoshiyasu; Takahashi, Takuya; Asahara, Takashi; Tsuji, Hirokazu; Tsuji, Noriko M; Kiyono, Hiroshi; Ma, Ji Su; Kusu, Takashi; Okumura, Ryu; Hara, Hiromitsu; Yoshida, Hiroki; Yamamoto, Masahiro; Nomoto, Koji; Takeda, Kiyoshi

    2012-01-01

    Specific intestinal microbiota has been shown to induce Foxp3(+) regulatory T cell development. However, it remains unclear how development of another regulatory T cell subset, Tr1 cells, is regulated in the intestine. Here, we analyzed the role of two probiotic strains of intestinal bacteria, Lactobacillus casei and Bifidobacterium breve in T cell development in the intestine. B. breve, but not L. casei, induced development of IL-10-producing Tr1 cells that express cMaf, IL-21, and Ahr in the large intestine. Intestinal CD103(+) dendritic cells (DCs) mediated B. breve-induced development of IL-10-producing T cells. CD103(+) DCs from Il10(-/-), Tlr2(-/-), and Myd88(-/-) mice showed defective B. breve-induced Tr1 cell development. B. breve-treated CD103(+) DCs failed to induce IL-10 production from co-cultured Il27ra(-/-) T cells. B. breve treatment of Tlr2(-/-) mice did not increase IL-10-producing T cells in the colonic lamina propria. Thus, B. breve activates intestinal CD103(+) DCs to produce IL-10 and IL-27 via the TLR2/MyD88 pathway thereby inducing IL-10-producing Tr1 cells in the large intestine. Oral B. breve administration ameliorated colitis in immunocompromised mice given naïve CD4(+) T cells from wild-type mice, but not Il10(-/-) mice. These findings demonstrate that B. breve prevents intestinal inflammation through the induction of intestinal IL-10-producing Tr1 cells.

  13. VULTURES: EXEGESIS OF A SYMBOL

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    Maura Andreoni

    2016-12-01

    Full Text Available Depending on the different cultures and historical periods, vultures have been considered both impure or sacred. But, since they usually do not prey upon living animals, their symbolic dimension, associated to the idea of purification, is present in many myths, religions, burial praxis of ancient populations and remains in some religions today.In the ancient Mediterranean civilizations, they have been carved in some of the most ancient bas-reliefs of the history by stone age people; were sacred to Egyptians, who even took them as symbol of gods; in the classical times they were supposed to be all feminine and breed by parthenogenesis, and therefore appreciated by some early Christian authors, who came to comparing them even to the Virgin Mary; they have been studied and described by ancient scientists, naturalists, philosophers, playwrights; involved in many of the most enduring Greek and Roman myths and legends; many parts of their body were considered as a medicine or even a talisman for happiness; and they were so proverbial for Romans to become even one of the symbols of the founding of Rome itself.But they were also so fragile that perfumes, myrrh and pomegranates were supposed to be lethal for them ….

  14. HIGH BREVETOXIN CONCENTRATIONS IN GYMNODINIUM BREVE BLOOMS ALONG THE NORTHWEST FLORIDA COAST DURING 1999.

    Science.gov (United States)

    Blooms of the dinoflagellate Gymnodinium breve (i.e. red tides) produce brevetoxins (PbTx) that negatively impact the Gulf of Mexico ecosystem, human health, and local economics. Characterizing and predicting bloom events and their impacts requires knowledge of G. breve abundance...

  15. La ANT tal como yo la imagino. Breve ensayo sobre el cosmos semafórico

    Directory of Open Access Journals (Sweden)

    Carlos Enrique Silva Rios

    2011-03-01

    Full Text Available Hace tres años llegué a Barcelona con una intención firme que pasados unos dos meses tuve que echar por tierra. Quería estudiar a la gente cruzando la calle en esta ciudad y luego comparar sus actos con los de la gente de mi ciudad de origen, Caracas. Mis evaluadores, apenas se enteraron de mi propósito, me desaconsejaron, ya que comparar requería de un tiempo que no tenía. Debía dedicarme sólo a una de las ciudades. Escogí Barcelona. Más tarde, en una segunda consulta, volvieron a desaconsejarme: en lugar de estudiar a la gente que cruza la calle, me recomendaban que tomara como punto de partida el semáforo; específicamente, el cambio de luz y si era posible la luz misma. Esta especie de atomización del interés, en lugar de amilanarme, redefinió y acicateó mi entusiasmo cogitativo, y con ese impulso emprendí la tarea de elaborar un diario de campo. Como no se podía hacer eso de una manera ingenua, tomé la decisión de asumir una mirada y luego una escritura sesgada por algunas de las nociones de la Teoría del Actor-Red tal como las plantea Bruno Latour. Su manera de ver el mundo, más que recibirla como un conjunto sistemático de conceptos, la he incorporado muy lentamente como un dispositivo de imaginación, es decir, como una serie de formas que promueven y facilitan la re-creación de los acontecimientos mundanos. En este ensayo hablo brevemente de parte de esa experiencia investigativa, centrándome en una idea que para mí no deja de ser fascinante: es posible componer un mundo común, un cosmos, partiendo de una entidad aparentemente discreta; en mi caso, el semáforo.

  16. Breve comentário sobre o artigo Gravura e desenho, de Ferreira Gullar

    Directory of Open Access Journals (Sweden)

    Renato Rodrigues da Silva

    2016-12-01

    Full Text Available Ferreira Gullar (1930 publicou o artigo intitulado “Gravura e Desenho” no Suplemento Dominical do Jornal do Brasil (SDJB, em 11 de novembro de 1956. Estamos apenas no segundo mês do seu trabalho de cinco anos como editor da seção de artes visuais do encarte, que se tornou órgão oficioso do Neoconcretismo anos depois. A excepcionalidade desse artigo deve-se não somente à sua idade, pois ele tinha vinte e seis anos, como também à qualidade da análise, que distinguiu dois tipos psicológicos – o do gravador e do desenhista – em função dos meios de expressão. A definição dos tipos psicológicos não deve distrair o leitor, pois o texto já fazia parte da renovação modernista que transformou a arte brasileira depois da II Guerra Mundial. Com a vitória do abstracionismo (geométrico e informal, um segmento da crítica nacional acreditou na autonomia do objeto, cujas características eram definidas por categorias constituídas, tais como pintura, escultura, gravura, desenho, arquitetura, etc.

  17. BREVES CONSIDERAÇÕES SOBRE A TEORIA PURA DE HANS KELSEN

    OpenAIRE

    Siqueira, Dirceu Pereira; Instituição Toledo de Ensino; Botelho, Marcos César; Instituição Toledo de Ensino

    2012-01-01

    O presente artigo visa discutir os principais aspectos da teoria pura de Hans Kelsen, tratando, especificamente, de dois pontos fundamentais de sua teoria, a saber, a estática jurídica e a dinâmica jurídica, objetivando fornecer um delineamento geral do modelo proposto pelo mestre de Viena.

  18. Breve comentário sobre o processo eleitoral de 2010

    Directory of Open Access Journals (Sweden)

    Pedro Vanzin Filho

    2010-12-01

    Full Text Available

    Neste ano de 2010, no qual tivemos eleições para escolha dos condutores máximos do Brasil, o processo encerrado a pouco ratificou a Democracia Representativa. Essa que não enseja a participação direta dos cidadãos nos processos de decisão manteve com a presença do eleitorado brasileiro a ordem constitucional vigente.

  19. Desde Prometeo hasta la proteomica, una breve historia de las ideas sobre el crecimiento tisular

    Directory of Open Access Journals (Sweden)

    Barbeito, Claudio Gustavo

    2010-01-01

    Full Text Available Durante muchos siglos, la regulación del crecimiento de los órganos y los tejidos se relacionó con su función y con la temperatura, esta última se asociaba con la llegada de sangre al órgano. Con el surgimiento de la biología experimental y la endocrinología, en la segunda mitad del siglo XIX, comenzaron a realizarse experimentos para explicar el crecimiento de los tejidos. Se estableció por entonces el efecto estimulante de las hormonas. En la primera mitad del siglo XX, surgieron distintos modelos que proponían la existencia de estimulantes e inhibidores del crecimiento, tanto de acción local como general. A partir de 1962 se desarrolló el concepto de chalonas para referirse a inhibidores del crecimiento específi cos de tejido pero no de especie, veinte años después el término cayó en el olvido para ser retomado recientemente, en una forma renovada. Con el descubrimiento de los factores de crecimiento, se demostró que las sustancias reguladoras son de acciones más variadas y complejas. El modelo actual de la regulación del crecimiento incluye numerosas sustancias de efectos y mecanismos de acción muy variados que a su vez están en relación con los efectos del medio ambiente.

  20. Breves comentários sobre as teorias marxistas de imigração

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    Raul Felix Barbosa

    2017-10-01

    Full Text Available Sabe-se que o capitalismo possui sua parcela de responsabilidade nos movimentos migratórios, voluntários ou forçados, do período pós-revolução industrial. Apesar de Karl Marx não ter abordado diretamente a questão migratório, a teoria marxista e neomarxista se ocupa de explicar os processos migratórios. Esse artigo realiza um levantamento das teorias migratórias de origem marxista apresentando-as, bem como as críticas recebidas.

  1. Breves anotações sobre o comércio eletrônico

    OpenAIRE

    Canella, Sérgio Eduardo; Universidade Estadual de Londrina; Lewis, Sandra Barbon; Universidade Estadual de Londrina

    2005-01-01

    O estudo verifica aspectos da concentração empresarial no comércio eletrônico. Para tanto, primeiramente, é realizada uma análise do papel das empresas no contexto global a partir do avanço tecnológico que é posto à disposição e da nova ordem mundial que lhe é apresentada. O fluxo instantâneo de informações, possibilitado pelo avanço da Internet e o alto grau de competitividade introduzido pela Globalização, levaram os agentes econômicos a buscar no comércio pela Internet uma nova perspectiva...

  2. Breve reseña histórica sobre tisiocirugía

    Directory of Open Access Journals (Sweden)

    Bárbaro Agustín Armas Pérez

    Full Text Available La tuberculosis o "peste blanca" fue un azote mundial, comparable hoy día con el síndrome de inmunodeficiencia adquirida, al que se asocia, pues "nunca enfermo alguno sufrió tanto ni fue tan despreciado". El 24 de marzo de 1882 Robert Koch mostró el bacilo que causaba la tisis o consunción, y desde entonces esa fecha se reconoce como el Día Mundial contra la Tuberculosis. Se presenta una revisión del tema ante el LIV Aniversario del Hospital "Amalia Simoni", inicialmente antituberculoso. El objetivo secundario es homenajear a todos sus trabajadores, en especial a los fundadores. Se describen los tipos de tratamiento quirúrgico utilizados a través del tiempo, desde la cavernotomía hasta las resecciones pulmonares. Muchas técnicas y principios modernos de la cirugía torácica se desarrollaron a partir de la tisiocirugía.

  3. Bifidobacterium breve attenuates murine dextran sodium sulfate-induced colitis and increases regulatory T cell responses.

    Science.gov (United States)

    Zheng, Bin; van Bergenhenegouwen, Jeroen; Overbeek, Saskia; van de Kant, Hendrik J G; Garssen, Johan; Folkerts, Gert; Vos, Paul; Morgan, Mary E; Kraneveld, Aletta D

    2014-01-01

    While some probiotics have shown beneficial effects on preventing or treating colitis development, others have shown no effects. In this study, we have assessed the immunomodulating effects of two probiotic strains, Lactobacillus rhamnosus (L. rhamnosus) and Bifidobacterium breve (B. breve) on T cell polarization in vitro, using human peripheral blood mononuclear cells (PBMC), and in vivo, using murine dextran sodium sulfate (DSS) colitis model. With respect to the latter, the mRNA expression of T cell subset-associated transcription factors and cytokines in the colon was measured and the T helper type (Th) 17 and regulatory T cell (Treg) subsets were determined in the Peyer's patches. Both L. rhamnosus and B. breve incubations in vitro reduced Th17 and increased Th2 cell subsets in human PBMCs. In addition, B. breve incubation was also able to reduce Th1 and increase Treg cell subsets in contrast to L. rhamnosus. In vivo intervention with B. breve, but not L. rhamnosus, significantly attenuated the severity of DSS-induced colitis. In DSS-treated C57BL/6 mice, intervention with B. breve increased the expression of mRNA encoding for Th2- and Treg-associated cytokines in the distal colon. In addition, intervention with B. breve led to increases of Treg and decreases of Th17 cell subsets in Peyer's patches of DSS-treated mice. B. breve modulates T cell polarization towards Th2 and Treg cell-associated responses in vitro and in vivo. In vivo B. breve intervention ameliorates DSS-induced colitis symptoms and this protective effect may mediated by its effects on the T-cell composition.

  4. Bifidobacterium breve attenuates murine dextran sodium sulfate-induced colitis and increases regulatory T cell responses.

    Directory of Open Access Journals (Sweden)

    Bin Zheng

    Full Text Available While some probiotics have shown beneficial effects on preventing or treating colitis development, others have shown no effects. In this study, we have assessed the immunomodulating effects of two probiotic strains, Lactobacillus rhamnosus (L. rhamnosus and Bifidobacterium breve (B. breve on T cell polarization in vitro, using human peripheral blood mononuclear cells (PBMC, and in vivo, using murine dextran sodium sulfate (DSS colitis model. With respect to the latter, the mRNA expression of T cell subset-associated transcription factors and cytokines in the colon was measured and the T helper type (Th 17 and regulatory T cell (Treg subsets were determined in the Peyer's patches. Both L. rhamnosus and B. breve incubations in vitro reduced Th17 and increased Th2 cell subsets in human PBMCs. In addition, B. breve incubation was also able to reduce Th1 and increase Treg cell subsets in contrast to L. rhamnosus. In vivo intervention with B. breve, but not L. rhamnosus, significantly attenuated the severity of DSS-induced colitis. In DSS-treated C57BL/6 mice, intervention with B. breve increased the expression of mRNA encoding for Th2- and Treg-associated cytokines in the distal colon. In addition, intervention with B. breve led to increases of Treg and decreases of Th17 cell subsets in Peyer's patches of DSS-treated mice. B. breve modulates T cell polarization towards Th2 and Treg cell-associated responses in vitro and in vivo. In vivo B. breve intervention ameliorates DSS-induced colitis symptoms and this protective effect may mediated by its effects on the T-cell composition.

  5. Safety of Bifidobacterium breve (BBG-01) in preterm infants.

    Science.gov (United States)

    Kitajima, Hiroyuki; Hirano, Shinya

    2017-03-01

    Since 1982, viable cells of Bifidobacterium breve (BBG-01) have been used in pediatric patients for clinical conditions such as intractable infantile diarrhea, preterm status, and pediatric surgery. Although the efficacy of BBG-01 has been widely reported, adverse events related to the use of BBG-01 have been reported in very few cases. In order to trace adverse events seen by 109 doctors in 88 medical institutions where BBG-01 was used, a questionnaire survey of the number of occurrences and details of each case was conducted. Eighty-six clinicians (70 institutions) responded to the questionnaire (response rate, 78.9%). Number of respondents according to department of diagnosis (no. BBG-01-treated infants) was as follows: pediatrics, 29 respondents (10 938 patients); premature and newborn medicine, 26 (10 677 patients); obstetrics and gynecology, 1 (1212 patients) and pediatric surgery, 22 (169 patients). More than 90% of the total BBG-01-treated patients (23 092 patients) were in the departments of premature and newborn medicine and pediatrics, and BBG-01 had been used mainly in preterm infants and children with intractable diarrhea. Adverse events occurred in two extremely premature infants with functional ileus due to starch aggregates as vehicle, and in two surgical neonates with bacteremia caused by B. breve genetically identical to BBG-01, and no serious adverse events with poor outcome were reported. Adverse events related to the use of BBG-01 have an extremely low incidence and are mild in severity, thus ensuring the superior safety of this preparation. © 2016 Japan Pediatric Society.

  6. Impacto de una intervención breve basada en mindfulness en niños: Un estudio piloto

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    Carlos García Rubio

    2016-01-01

    Full Text Available En la última década el interés por los efectos de la práctica de mindfulness ha sido exponencial. Sin embargo, todavía es escasa la investigación sobre sus efectos en el contexto escolar. Este estudio piloto examinó la efectividad de una intervención breve basada en mindfulness en estudiantes de primaria. Se diseñó un estudio pre-post aleatorizado a nivel de clase con un grupo control. Se investigaron sus efectos sobre distintos síntomas internalizantes y externalizantes, las habilidades de mindfulness y el rendimiento académico. El grupo de mindfulness comparado con el grupo de control disminuyó sus puntuaciones en problemas de conducta, aumentó en relajación y mostró una mejora en sus relaciones sociales. Su rendimiento académico se mantuvo, mientras en el grupo control descendió. Se discuten posibles explicaciones y líneas futuras de investigación.

  7. Is Bifidobacterium breve effective in the treatment of childhood constipation? Results from a pilot study

    NARCIS (Netherlands)

    Tabbers, M. M.; de Milliano, I.; Roseboom, M. G.; Benninga, M. A.

    2011-01-01

    Probiotics are increasingly used in the treatment of functional gastrointestinal disorders. Studies in constipated adults with a Bifidus yoghurt (containing Bifidobacterium breve, Bifidobacterium bifidum and Lactobacillus acidophilus) showed a significant increase in defecation frequency. The aim of

  8. Capability of the two microorganisms Bifidobacterium breve B632 and Bifidobacterium breve BR03 to colonize the intestinal microbiota of children.

    Science.gov (United States)

    Mogna, Luca; Del Piano, Mario; Mogna, Giovanni

    2014-01-01

    The total number of bacteria present in the gut microbiota of a newborn is consistently lower than the average found in adults, with the extent of this difference being directly related to body weight and age. It could be assumed that a lower number of viable probiotic cells is necessary to achieve significant gut colonization in infants and children. This study assessed the capability of Bifidobacterium breve B632 (DSM 24706) and Bifidobacterium breve BR03 (DSM 16604), 2 strains able to significantly inhibit some gram-negative bacteria in vitro, to integrate into the intestinal microbiota of children. Ten healthy children aged an average of 5.7±2.6 were given an oily suspension containing B. breve B632 and B. breve BR03 for 21 consecutive days. The daily dose was 100 million live cells of each strain. Fecal specimens were collected and analyzed at the beginning (d0) and at the end of the study (d21). Total fecal bifidobacteria and coliforms have been quantified by microbiological plate counts. A significant increase in total fecal bifidobacteria (from 8.99 to 9.47 log10 CFU/g, P=0.042) and a parallel decrease in total coliforms (from 8.60 to 7.93 log10 CFU/g, P=0.048) was recorded after 21 days of supplementation. An oily suspension has proved an effective way of providing probiotics to children. A lower viable cells concentration was sufficient to mediate this effect in the light of the fact that the intestinal microbiota of children harbors a considerably smaller amount of total bacteria compared with adults. In addition to gut colonization in healthy children, B. breve B632 and B. breve BR03 were able to decrease total fecal coliforms, therefore supporting their potential specific use in colicky infants.

  9. Anti-Inflammatory Effects of Lactobacillus Rahmnosus and Bifidobacterium Breve on Cigarette Smoke Activated Human Macrophages

    OpenAIRE

    Mortaz, E; Adcock, IM; Ricciardolo, FLM; Varahram, M; Jamaati, H; Velayati, AA; Folkerts, G; Garssen, J

    2015-01-01

    BACKGROUND: Chronic obstructive pulmonary disease (COPD) is a major global health problem with cigarette smoke (CS) as the main risk factor for its development. Airway inflammation in COPD involves the increased expression of inflammatory mediators such as CXCL-8 and IL-1β which are important mediators for neutrophil recruitment. Macrophages are an important source of these mediators in COPD. Lactobacillus rhamnosus (L. rhamnosus) and Befidobacterium breve (B. breve) attenuate the development...

  10. Effects of Bifidobacterium Breve Feeding Strategy and Delivery Modes on Experimental Allergic Rhinitis Mice.

    Directory of Open Access Journals (Sweden)

    Jian-jun Ren

    Full Text Available Different delivery modes may affect the susceptibility to allergic diseases. It is still unknown whether early intervention with probiotics would counteract this effect.The effect of different delivery modes on immune status and nasal symptoms was investigated on established allergic rhinitis (AR mouse model. In addition, the immunoregulatory effects and mechanisms of different feeding manners with Bifidobacterium breve(B. breve were examined.Live lyophilized B. breve was orally administered to BALB/c mice born via vaginal delivery(VD or cesarean delivery (CD for 8 consecutive weeks, after which they were sensitized by ovalbumin(OVA to establish experimental AR. Nasal symptoms, serum immunoglobulins, cytokines, splenic percentages of CD4(+CD25(+Foxp3(+ regulatory T(Treg cells and nasal eosinophil infiltration were evaluated.Compared with VD mice, mice delivered via CD demonstrated more serious nasal symptoms, higher concentrations of OVA-specific immunoglobulin (Ig E, more nasal eosinophils and lower percentages of splenic CD4(+CD25(+Foxp3(+Treg cells after establishing experimental AR. These parameters were reversed by administering B. breves hortly after birth. However, the effect of B. breve did not differ between different delivery modes.CD aggravates the nasal symptoms of AR mice compared to VD. This is the first report that oral administration of B. breve shortly after birth can significantly alleviate the symptoms of AR mice born via both deliveries, probably via activation of the regulatory capacity of CD4(+CD25(+Foxp3(+Treg cells.

  11. Bifidobacterium breve prevents necrotising enterocolitis by suppressing inflammatory responses in a preterm rat model.

    Science.gov (United States)

    Satoh, T; Izumi, H; Iwabuchi, N; Odamaki, T; Namba, K; Abe, F; Xiao, J Z

    2016-02-01

    Necrotising enterocolitis (NEC) is associated with inflammatory responses and barrier dysfunction in the gut. In this study, we investigated the effect of Bifidobacterium breve M-16V on factors related to NEC development using an experimental rat model. Caesarean-sectioned rats were given formula milk with or without B. breve M-16V by oral gavage thrice daily, and experimental NEC was induced by exposing the rats to hypoxic conditions. Naturally delivered rats that were reared by their mother were used as healthy controls. The pathological score of NEC and the expression of molecules related to inflammatory responses and the barrier function were assessed in the ileum. B. breve M-16V reduced the pathological scores of NEC and resulted in some improvement in survivability. B. breve M-16V suppressed the increased expression of molecules related to inflammation and barrier function that resulted from NEC induction. B. breve M-16V normalised Toll-like receptor (TRL)4 expression and enhanced TLR2 expression. Our data suggest that B. breve M-16V prevents NEC development by modulating TLR expressions and suppressing inflammatory responses in a rat model.

  12. Effects of Bifidobacterium Breve Feeding Strategy and Delivery Modes on Experimental Allergic Rhinitis Mice.

    Science.gov (United States)

    Ren, Jian-jun; Yu, Zhao; Yang, Feng-Ling; Lv, Dan; Hung, Shi; Zhang, Jie; Lin, Ping; Liu, Shi-Xi; Zhang, Nan; Bachert, Claus

    2015-01-01

    Different delivery modes may affect the susceptibility to allergic diseases. It is still unknown whether early intervention with probiotics would counteract this effect. The effect of different delivery modes on immune status and nasal symptoms was investigated on established allergic rhinitis (AR) mouse model. In addition, the immunoregulatory effects and mechanisms of different feeding manners with Bifidobacterium breve(B. breve) were examined. Live lyophilized B. breve was orally administered to BALB/c mice born via vaginal delivery(VD) or cesarean delivery (CD) for 8 consecutive weeks, after which they were sensitized by ovalbumin(OVA) to establish experimental AR. Nasal symptoms, serum immunoglobulins, cytokines, splenic percentages of CD4(+)CD25(+)Foxp3(+) regulatory T(Treg) cells and nasal eosinophil infiltration were evaluated. Compared with VD mice, mice delivered via CD demonstrated more serious nasal symptoms, higher concentrations of OVA-specific immunoglobulin (Ig) E, more nasal eosinophils and lower percentages of splenic CD4(+)CD25(+)Foxp3(+)Treg cells after establishing experimental AR. These parameters were reversed by administering B. breves hortly after birth. However, the effect of B. breve did not differ between different delivery modes. CD aggravates the nasal symptoms of AR mice compared to VD. This is the first report that oral administration of B. breve shortly after birth can significantly alleviate the symptoms of AR mice born via both deliveries, probably via activation of the regulatory capacity of CD4(+)CD25(+)Foxp3(+)Treg cells.

  13. Sobre la muerte voluntaria

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    Juan Álvarez-Cienfuegos Fidalgo

    2012-03-01

    Full Text Available El problema de cómo morir en nuestra sociedad se ha convertido en una inquietud para los ciudadanos y la reflexión moral. Especialmcnte por dos razones: por el desarrollo de la tecnología médica y por el hecho de que la mayor parte de los fallecimientos tienen lugar fuera del hogar, donde tradicionalmente ocurra el deceso. El presente artículo tiene como objetivo acercarse a esta cuestión. Para ello, se parte de la autonomía del ciudadano, con derecho a decidir sobre lo que considera cuál sea su manera de vivir y de morir. A tal fin, se hace una breve introducción sobre el dolor, el suicidio y la muerte, desde un punto de vista antropológico, pasando, después, a mostrar las cuestiones relacionadas con el problema de la eutanasia, donde la atención se dirigirá, ante todo, al enjuiciamiento crítico del tenso equilibrio en el que se mantienen dos principios fundamentales de la bioética: el de no maleficencia y el de autonomía del ciudadano.The problem of how to die in modern society has become a concern for both the citizen and moral reflection for two reasons in particular: due to developments in medical technology, and because most deaths occur outside the honre, where they traditionaÍlY took place. This paper addresses this issue, starting from citizen autonomy, the right to decide on what s/he considers to be his or her way of living and dying. To this end, a brief introduction considers pain, suicide and death from an anthropological viewpoint. Questions related to the problem of euthanasia are then exposed, with particular attention to, above all the critical judgement of the tense balance in which two fundamental principies of bioethics are held, the principle of non-maleficence and that of citizen autonomy.

  14. Bifidobacterium breve - HT-29 cell line interaction: modulation of TNF-α induced gene expression.

    Science.gov (United States)

    Boesten, R J; Schuren, F H J; Willemsen, L E M; Vriesema, A; Knol, J; De Vos, W M

    2011-06-01

    To provide insight in the molecular basis for intestinal host-microbe interactions, we determined the genome-wide transcriptional response of human intestinal epithelial cells following exposure to cells of Bifidobacterium breve. To select an appropriate test system reflecting inflammatory conditions, the responsiveness to TNF-α was compared in T84, Caco-2 and HT-29 cells. The highest TNF-α response was observed in HT-29 cells and this cell line was selected for exposure to the B. breve strains M-16V, NR246 and UCC2003. After one hour of bacterial pre-incubation followed by two hours of additional TNF-α stimulation, B. breve M-16V (86%), but to a much lesser extent strains NR246 (50%) or UCC2003 (32%), showed a strain-specific reduction of the HT-29 transcriptional response to the inflammatory treatment. The most important functional groups of genes that were transcriptionally suppressed by the presence of B. breve M-16V, were found to be involved in immune regulation and apoptotic processes. About 54% of the TNF-α induced genes were solely suppressed by the presence of B. breve M-16V. These included apoptosis-related cysteine protease caspase 7 (CASP7), interferon regulatory factor 3 (IRF3), amyloid beta (A4) precursor proteinbinding family A member 1 (APBA1), NADPH oxidase (NOX5), and leukemia inhibitory factor receptor (LIFR). The extracellular IL-8 concentration was determined by an immunological assay but did not change significantly, indicating that B. breve M-16V only partially modulates the TNF-α pathway. In conclusion, this study shows that B. breve strains modulate gene expression in HT-29 cells under inflammatory conditions in a strain-specific way.

  15. Variation in consumption of human milk oligosaccharides by infant gut-associated strains of Bifidobacterium breve.

    Science.gov (United States)

    Ruiz-Moyano, Santiago; Totten, Sarah M; Garrido, Daniel A; Smilowitz, Jennifer T; German, J Bruce; Lebrilla, Carlito B; Mills, David A

    2013-10-01

    Human milk contains a high concentration of complex oligosaccharides that influence the composition of the intestinal microbiota in breast-fed infants. Previous studies have indicated that select species such as Bifidobacterium longum subsp. infantis and Bifidobacterium bifidum can utilize human milk oligosaccharides (HMO) in vitro as the sole carbon source, while the relatively few B. longum subsp. longum and Bifidobacterium breve isolates tested appear less adapted to these substrates. Considering the high frequency at which B. breve is isolated from breast-fed infant feces, we postulated that some B. breve strains can more vigorously consume HMO and thus are enriched in the breast-fed infant gastrointestinal tract. To examine this, a number of B. breve isolates from breast-fed infant feces were characterized for the presence of different glycosyl hydrolases that participate in HMO utilization, as well as by their ability to grow on HMO or specific HMO species such as lacto-N-tetraose (LNT) and fucosyllactose. All B. breve strains showed high levels of growth on LNT and lacto-N-neotetraose (LNnT), and, in general, growth on total HMO was moderate for most of the strains, with several strain differences. Growth and consumption of fucosylated HMO were strain dependent, mostly in isolates possessing a glycosyl hydrolase family 29 α-fucosidase. Glycoprofiling of the spent supernatant after HMO fermentation by select strains revealed that all B. breve strains can utilize sialylated HMO to a certain extent, especially sialyl-lacto-N-tetraose. Interestingly, this specific oligosaccharide was depleted before neutral LNT by strain SC95. In aggregate, this work indicates that the HMO consumption phenotype in B. breve is variable; however, some strains display specific adaptations to these substrates, enabling more vigorous consumption of fucosylated and sialylated HMO. These results provide a rationale for the predominance of this species in breast-fed infant feces and

  16. Anti-Inflammatory Effects of Lactobacillus Rahmnosus and Bifidobacterium Breve on Cigarette Smoke Activated Human Macrophages.

    Science.gov (United States)

    Mortaz, Esmaeil; Adcock, Ian M; Ricciardolo, Fabio L M; Varahram, Mohammad; Jamaati, Hamidreza; Velayati, Ali Akbar; Folkerts, Gert; Garssen, Johan

    2015-01-01

    Chronic obstructive pulmonary disease (COPD) is a major global health problem with cigarette smoke (CS) as the main risk factor for its development. Airway inflammation in COPD involves the increased expression of inflammatory mediators such as CXCL-8 and IL-1β which are important mediators for neutrophil recruitment. Macrophages are an important source of these mediators in COPD. Lactobacillus rhamnosus (L. rhamnosus) and Befidobacterium breve (B. breve) attenuate the development of 'allergic asthma' in animals but their effects in COPD are unknown. To determine the anti-inflammatory effects of L. rhamnosus and B. breve on CS and Toll-like receptor (TLR) activation. We stimulated the human macrophage cell line THP-1 with CS extract in the presence and absence of L. rhamnosus and B. breve and measured the expression and release of inflammatory mediators by RT-qPCR and ELISA respectively. An activity assay and Western blotting were used to examine NF-κB activation. Both L. rhamnosus and B. breve were efficiently phagocytized by human macrophages. L. rhamnosus and B. breve significantly suppressed the ability of CS to induce the expression of IL-1β, IL-6, IL-10, IL-23, TNFα, CXCL-8 and HMGB1 release (all p<0.05) in human THP-1 macrophages. Similar suppression of TLR4- and TLR9-induced CXCL8 expression was also observed (p<0.05). The effect of L. rhamnosus and B. breve on inflammatory mediator release was associated with the suppression of CS-induced NF-κB activation (p<0.05). This data indicate that these probiotics may be useful anti-inflammatory agents in CS-associated disease such as COPD.

  17. Anti-Inflammatory Effects of Lactobacillus Rahmnosus and Bifidobacterium Breve on Cigarette Smoke Activated Human Macrophages.

    Directory of Open Access Journals (Sweden)

    Esmaeil Mortaz

    Full Text Available Chronic obstructive pulmonary disease (COPD is a major global health problem with cigarette smoke (CS as the main risk factor for its development. Airway inflammation in COPD involves the increased expression of inflammatory mediators such as CXCL-8 and IL-1β which are important mediators for neutrophil recruitment. Macrophages are an important source of these mediators in COPD. Lactobacillus rhamnosus (L. rhamnosus and Befidobacterium breve (B. breve attenuate the development of 'allergic asthma' in animals but their effects in COPD are unknown.To determine the anti-inflammatory effects of L. rhamnosus and B. breve on CS and Toll-like receptor (TLR activation.We stimulated the human macrophage cell line THP-1 with CS extract in the presence and absence of L. rhamnosus and B. breve and measured the expression and release of inflammatory mediators by RT-qPCR and ELISA respectively. An activity assay and Western blotting were used to examine NF-κB activation.Both L. rhamnosus and B. breve were efficiently phagocytized by human macrophages. L. rhamnosus and B. breve significantly suppressed the ability of CS to induce the expression of IL-1β, IL-6, IL-10, IL-23, TNFα, CXCL-8 and HMGB1 release (all p<0.05 in human THP-1 macrophages. Similar suppression of TLR4- and TLR9-induced CXCL8 expression was also observed (p<0.05. The effect of L. rhamnosus and B. breve on inflammatory mediator release was associated with the suppression of CS-induced NF-κB activation (p<0.05.This data indicate that these probiotics may be useful anti-inflammatory agents in CS-associated disease such as COPD.

  18. Psicoterapia breve pais-bebê: revisando a literatura Short-term parent-infant psychotherapy: a review of the literature

    Directory of Open Access Journals (Sweden)

    Luiz Carlos Prado

    2009-01-01

    Full Text Available Este artigo apresenta uma revisão da literatura a respeito da psicoterapia breve pais-bebê. Inicialmente são apresentados aspectos históricos, desde o surgimento da psicoterapia breve, passando pelas formulações teóricas que enfatizaram a importância das relações iniciais pais-bebê até a constituição do campo da psicoterapia breve pais-bebê propriamente dita. Constata-se que várias abordagens compõem o panorama atual das psicoterapias pais-bebê, as quais apresentam uma ampla diversidade de referenciais teóricos e técnicos. Foram também revisados, em particular, estudos empíricos sobre a utilização dessa abordagem no contexto da depressão materna. Por fim, apresentam-se os procedimentos utilizados pelos autores deste artigo em uma pesquisa em andamento envolvendo o atendimento psicoterápico breve pais-bebê em famílias com mães deprimidas. Essa abordagem tem se mostrado efetiva na promoção de um melhor relacionamento mãe-pai-bebê na presença sintomas depressivos da mãe.This article presents a review of the literature on short-term parent-infant psychotherapy. Initially, some historical aspects are presented since the emergence of short-term psychotherapy, including the theoretical formulations that highlighted the importance of early parent-infant relationships and helped to implement the field of short-term parent-infant psychotherapy. There are several approaches to parent-infant psychotherapy, representing a broad diversity of theoretical and technical frameworks. Empirical studies were also reviewed, especially those using parent-infant psychotherapy in the context of maternal depression. Finally, the authors' experience with short-term parent-infant psychotherapy involving families with depressive mothers was presented. This approach has shown to be effective in the promotion of better mother-father-infant relationship in the presence of maternal depressive symptoms.

  19. Effect of Bifidobacterium breve B-3 on skin photoaging induced by chronic UV irradiation in mice.

    Science.gov (United States)

    Satoh, T; Murata, M; Iwabuchi, N; Odamaki, T; Wakabayashi, H; Yamauchi, K; Abe, F; Xiao, J Z

    2015-01-01

    Probiotics have been shown to have a preventative effect on skin photoaging induced by short term UV irradiation, however, the underlying mechanisms and the effect of probiotics on skin photoaging induced by chronic UV irradiation remain unclear. In this study, we investigated the effect of Bifidobacterium breve B-3 on skin photoaging induced by chronic UV irradiation in hairless mice. Mice were irradiated with UVB three times weekly and orally administered B. breve B-3 (2×10(9) cfu/mouse /day) for 7 weeks. Nonirradiated mice and UVB-irradiated mice without probiotic treatment were used as controls. B. breve B-3 significantly suppressed the changes of transepidermal water loss, skin hydration, epidermal thickening and attenuated the damage to the tight junction structure and basement membrane induced by chronic UVB irradiation. Administration of B. breve B-3 tended to suppress the UV-induced interleukin-1β production in skin (P=0.09). These results suggest that B. breve B-3 could potentially be used to prevent photoaging induced by chronic UV irradiation.

  20. Administration of Bifidobacterium breve Decreases the Production of TNF-α in Children with Celiac Disease.

    Science.gov (United States)

    Klemenak, Martina; Dolinšek, Jernej; Langerholc, Tomaž; Di Gioia, Diana; Mičetić-Turk, Dušanka

    2015-11-01

    Increasing evidence suggests that not only genetics, but also environmental factors like gut microbiota dysbiosis play an important role in the pathogenesis of celiac disease (CD). The aim of our study was to investigate the effect of two probiotic strains Bifidobacterium breve BR03 and B. breve B632 on serum production of anti-inflammatory cytokine interleukin 10 (IL-10) and pro-inflammatory cytokine tumor necrosis factor alpha (TNF-α) in children with CD. The study was a double-blinded, placebo-controlled trial that included 49 children with CD on gluten-free diet (GFD) randomized into two groups and 18 healthy children in the control group. The first group (24 children with CD) daily received B. breve BR03 and B632 (2 × 10(9) colony-forming units) and the second group (25 children with CD) received placebo for 3 months. TNF-α levels were significantly decreased in the first group after receiving B. breve for 3 months. On follow-up, 3 months after receiving probiotics, TNF-α levels increased again. Children with CD who were on GFD for less than 1 year showed similar baseline TNF-α levels as children who were on GFD for more than 1 year. IL-10 levels were in all groups of patients below detection level. Probiotic intervention with B. breve strains has shown a positive effect on decreasing the production of pro-inflammatory cytokine TNF-α in children with CD on GFD.

  1. Therapeutic potential of Bifidobacterium breve strain A1 for preventing cognitive impairment in Alzheimer's disease.

    Science.gov (United States)

    Kobayashi, Yodai; Sugahara, Hirosuke; Shimada, Kousuke; Mitsuyama, Eri; Kuhara, Tetsuya; Yasuoka, Akihito; Kondo, Takashi; Abe, Keiko; Xiao, Jin-Zhong

    2017-10-18

    It has previously been shown that the consumption of probiotics may have beneficial effects not only on peripheral tissues but also on the central nervous system and behavior via the microbiota-gut-brain axis, raising the possibility that treatment with probiotics could be an effective therapeutic strategy for managing neurodegenerative disorders. In this study, we investigated the effects of oral administration of Bifidobacterium breve strain A1 (B. breve A1) on behavior and physiological processes in Alzheimer's disease (AD) model mice. We found that administration of B. breve A1 to AD mice reversed the impairment of alternation behavior in a Y maze test and the reduced latency time in a passive avoidance test, indicating that it prevented cognitive dysfunction. We also demonstrated that non-viable components of the bacterium or its metabolite acetate partially ameliorated the cognitive decline observed in AD mice. Gene profiling analysis revealed that the consumption of B. breve A1 suppressed the hippocampal expressions of inflammation and immune-reactive genes that are induced by amyloid-β. Together, these findings suggest that B. breve A1 has therapeutic potential for preventing cognitive impairment in AD.

  2. Notas sobre publicaciones medievales portuguesas

    Directory of Open Access Journals (Sweden)

    José Luis MARTÍN

    2009-11-01

    Full Text Available Contra lo que debiera suceder, la proximidad con Portugal no incita al conocimiento de su historia y son muy raros los manuales de Historia de España en los que se concede a la Edad Media Portuguesa la importancia que realmente tiene, y en los tratados de Historia Medieval Europea el espacio dedicado a Portugal es mínimo, al menos hasta la época de los Descubrimientos; por otra parte, son contados los medievalistas españoles que se interesan por la historia de Portugal. Ante estos silencios y ante la escasa presencia en las bibliotecas españolas de obras portuguesas el estudiante de historia puede pensar que el país vecino carece de medievalistas de relieve, que su historia medieval está por hacer... y nada más lejos de la realidad como intentaré probar en este breve comentario, sin ánimo exhaustivo, sobre algunas de las últimas publicaciones aparecidas en Portugal.

  3. Metabolism of four α-glycosidic linkage-containing oligosaccharides by Bifidobacterium breve UCC2003.

    Science.gov (United States)

    O'Connell, Kerry Joan; O'Connell Motherway, Mary; O'Callaghan, John; Fitzgerald, Gerald F; Ross, R Paul; Ventura, Marco; Stanton, Catherine; van Sinderen, Douwe

    2013-10-01

    Members of the genus Bifidobacterium are common inhabitants of the gastrointestinal tracts of humans and other mammals, where they ferment many diet-derived carbohydrates that cannot be digested by their hosts. To extend our understanding of bifidobacterial carbohydrate utilization, we investigated the molecular mechanisms by which 11 strains of Bifidobacterium breve metabolize four distinct α-glucose- and/or α-galactose-containing oligosaccharides, namely, raffinose, stachyose, melibiose, and melezitose. Here we demonstrate that all B. breve strains examined possess the ability to utilize raffinose, stachyose, and melibiose. However, the ability to metabolize melezitose was not common to all B. breve strains tested. Transcriptomic and functional genomic approaches identified a gene cluster dedicated to the metabolism of α-galactose-containing carbohydrates, while an adjacent gene cluster, dedicated to the metabolism of α-glucose-containing melezitose, was identified in strains that are able to use this carbohydrate.

  4. Ability of Bifidobacterium breve To Grow on Different Types of Milk: Exploring the Metabolism of Milk through Genome Analysis ▿

    Science.gov (United States)

    Turroni, Francesca; Foroni, Elena; Serafini, Fausta; Viappiani, Alice; Montanini, Barbara; Bottacini, Francesca; Ferrarini, Alberto; Bacchini, Pier Luigi; Rota, Claudio; Delledonne, Massimo; Ottonello, Simone; van Sinderen, Douwe; Ventura, Marco

    2011-01-01

    We have investigated the occurrence of bifidobacteria in human milk samples, and we provide evidence regarding the predominance of members of the Bifidobacterium breve species in this environment. Moreover, evaluation of the growth capabilities and transcriptomic analyses of one representative isolate of this species, i.e., B. breve 4L, on different milk types were performed. PMID:21856831

  5. Post-sensitization administration of non-digestible oligosaccharides and Bifidobacterium breve M-16V reduces allergic symptoms in mice

    NARCIS (Netherlands)

    van Esch, Betty C A M; Abbring, Suzanne; Diks, Mara A P; Dingjan, Gemma M; Harthoorn, Lucien F; Vos, A Paul; Garssen, Johan

    To support dietary management of severe cow's milk allergic infants, a synbiotic mixture of non-digestible oligosaccharides and Bifidobacterium breve M-16V (B. breve) was designed from source materials that are completely cow's milk-free. It was investigated whether this specific synbiotic concept

  6. Ability of Bifidobacterium breve to grow on different types of milk: exploring the metabolism of milk through genome analysis.

    Science.gov (United States)

    Turroni, Francesca; Foroni, Elena; Serafini, Fausta; Viappiani, Alice; Montanini, Barbara; Bottacini, Francesca; Ferrarini, Alberto; Bacchini, Pier Luigi; Rota, Claudio; Delledonne, Massimo; Ottonello, Simone; van Sinderen, Douwe; Ventura, Marco

    2011-10-01

    We have investigated the occurrence of bifidobacteria in human milk samples, and we provide evidence regarding the predominance of members of the Bifidobacterium breve species in this environment. Moreover, evaluation of the growth capabilities and transcriptomic analyses of one representative isolate of this species, i.e., B. breve 4L, on different milk types were performed.

  7. Synbiotic promotion of epithelial proliferation by orally ingested encapsulated Bifidobacterium breve and raffinose in the small intestine of rats.

    Science.gov (United States)

    Ishizuka, Satoshi; Iwama, Ami; Dinoto, Achmad; Suksomcheep, Akarat; Maeta, Kohshi; Kasai, Takanori; Hara, Hiroshi; Yokota, Atsushi

    2009-05-01

    We evaluated the effects of Bifidobacterium breve JCM1192(T )and/or raffinose on epithelial proliferation in the rat small and large intestines. WKAH/Hkm Slc rats (4 wk old) were fed a control diet, a diet supplemented with either encapsulated B. breve (30 g/kg diet, 1.5 x 10(7) colony-forming unit/g capsule) or raffinose (30 g/kg diet), or a diet supplemented with both encapsulated B. breve and raffinose, for 3 wk. Epithelial proliferation in the small intestine, as assessed by bromodeoxyuridine immunohistochemistry, was increased only in the B. breve plus raffinose-fed group. We determined the number of bifidobacteria in cecal contents using fluorescence in situ hybridization and confirmed the presence of ingested B. breve only in the B. breve plus raffinose-fed group. This suggests that the ingested B. breve cells used raffinose and were activated in the small intestine, where they subsequently influenced epithelial proliferation. In conclusion, we found a prominent synbiotic effect of encapsulated B. breve in combination with raffinose on epithelial proliferation in rat small intestine but not in large intestine. To our knowledge, this is the first report of a synbiotic that affects epithelial proliferation.

  8. PHYLOGENETIC RELATIONSHIP OF THE RED TIDE DINOFLAGELLATE GYMNODINIUM BREVE TO OTHER MEMBERS OF THE GENERA GYMNODINIUM AND GYRODINIUM

    Science.gov (United States)

    Phylogenetic relationships between the red-tide dinoflagellate Gymnodinium breve and other members of the genera Gymnodinium and Gyrodinium have not been studied at the molecular level. G. breve is most noted for its production of brevetoxin, which has been linked to extensive f...

  9. Nota breve del editor y traductor de la «Ética nicomaquea»

    OpenAIRE

    Batalla, Josep

    2017-01-01

    Una breu exposició de les dificultats en l'edició de l'Ètica nicomaquea d'Aristòtil i una breu presentació del mode de resoldre-les escollit per l'editor. This note provides a summary explanation of the difficulties in editing the Nicomachean Ethics of Aristotle and a brief presentation of the way the editor has chosen to resolve them. Una breve exposición de las dificultades en la edición de la Ética nicomaquea de Aristóteles y una breve presentación del modo de resolverlas escogido po...

  10. Benefits of Bifidobacterium breve M-16V Supplementation in Preterm Neonates - A Retrospective Cohort Study.

    Science.gov (United States)

    Patole, Sanjay K; Rao, Shripada C; Keil, Anthony D; Nathan, Elizabeth A; Doherty, Dorota A; Simmer, Karen N

    2016-01-01

    Systematic reviews of randomised controlled trials report that probiotics reduce the risk of necrotising enterocolitis (NEC) in preterm neonates. To determine whether routine probiotic supplementation (RPS) to preterm neonates would reduce the incidence of NEC. The incidence of NEC ≥ Stage II and all-cause mortality was compared for an equal period of 24 months 'before' (Epoch 1) and 'after' (Epoch 2) RPS with Bifidobacterium breve M-16V in neonates Bifidobacterium breve M-16V was associated with decreased NEC≥ Stage II and 'NEC≥ Stage II or all-cause mortality' in neonates <34 weeks. Large sample size is required to assess the potential benefits of RPS in neonates <28 weeks.

  11. Identification and characterization of an oleate hydratase-encoding gene from Bifidobacterium breve.

    Science.gov (United States)

    O'Connell, Kerry Joan; Motherway, Mary O'Connell; Hennessey, Alan A; Brodhun, Florian; Ross, R Paul; Feussner, Ivo; Stanton, Catherine; Fitzgerald, Gerald F; van Sinderen, Douwe

    2013-01-01

    Bifidobacteria are common commensals of the mammalian gastrointestinal tract. Previous studies have suggested that a bifidobacterial myosin cross reactive antigen (MCRA) protein plays a role in bacterial stress tolerance, while this protein has also been linked to the biosynthesis of conjugated linoleic acid (CLA) in bifidobacteria. In order to increase our understanding on the role of MCRA in bifidobacteria we created and analyzed an insertion mutant of the MCRA-encoding gene of B. breve NCFB 2258. Our results demonstrate that the MCRA protein of B. breve NCFB 2258 does not appear to play a role in CLA production, yet is an oleate hydratase, which contributes to bifidobacterial solvent stress protection.

  12. Lysis of Gymnodinium breve by cultures of the green alga Nannochloris eucaryotum.

    Science.gov (United States)

    Pérez, E; Sawyers, W G; Martin, D F

    2001-01-01

    Laboratory cultures of Florida's red tide organism, Gymnodinium breve, were killed by the green alga Nannochloris eucaryotum. Studies involved organism-organism interaction as well as organism-cell-free culture (N. eucaryotum) interaction. Both studies demonstrated that N. eucaryotum adversely affected Florida's red tide organism. The lysis has been attributed to compounds called APONINs (apparent oceanic naturally occurring cytolins). N. eucaryotum crude APONIN was extracted from cell-free cultures, partially purified and fractionated. The fractions were bioassayed against G. breve, and 'fingerprints' of the deleterious fractions were obtained.

  13. Breve estudio comparativo de los modernos medios de transporte terrestre

    Directory of Open Access Journals (Sweden)

    de Andrés Conde, M. Carmen

    1974-04-01

    Full Text Available Interurban Planning Section General Division of Highways and Subsidiary Roads In this study a series of data regarding the modern means of transport, available at present, are being compiled and compared in order to facilitate the choice of the most suitable one for each case. In order to understand better the various using conditions we have listed the most prominent known characteristics of each of them in alphabetical order in the definitions. With some of these characteristics as a point of departure, such as speed and capacity we have obtained criteria as to the application field of every means of transport.En este estudio se recopilan y comparan una serie de datos actualmente disponibles sobre modernos medios de transporte terrestre, con objeto de facilitar la elección del más adecuado en cada caso. Para ayudar a la comprensión de sus condiciones de explotación, se han resumido en las definiciones las características conocidas más destacadas de cada uno de ellos, ordenándolos por orden alfabético. A partir de algunas de estas características de explotación, como velocidad y capacidad, se han obtenido criterios sobre el campo de aplicación de cada medio de transporte.

  14. Últimos trabajos sobre ladrillos huecos y perforados

    Directory of Open Access Journals (Sweden)

    Editorial, Equipo

    1958-10-01

    Full Text Available Not availableEn la Building Research Station se están llevando a cabo, cada día con resultados positivos más alentadores, investigaciones sobre nuevos tipos de ladrillos huecos y perforados. Es de esperar que, en breve, todos estos estudios den lugar a la aparición de unas normas para su empleo en escala industrial y, precisamente ahora, es, en Inglaterra, el momento oportuno, puesto que, actualmente, está celebrando reuniones la Sociedad Cerámica Británica. Lo que se publica en este artículo es un anticipo sobre las tendencias actuales dentro da este campo.

  15. La terapia breve: una alternativa de intervención psicológica efectiva

    Directory of Open Access Journals (Sweden)

    Nohelia Hewitt Ramírez

    2009-10-01

    Full Text Available El objetivo de este trabajo es describir y estructurar los orígenes, bases teóricas y los procedimientos de la terapia breve desde la perspectiva terapéutica cognitivo conductual. Se inicia con la identificación de las bases teóricas de la terapia breve, principalmente las teorías motivacionales relacionadas con la disponibilidad al cambio y la autoeficacia desde la teoría social cognitiva. Se realiza un paralelo entre las características principales de la terapia breve en contraposición con las terapias tradicionales. Por último, se propone una estructura procedimental general de estrategias clínicas a emplear en la terapia breve con soporte empírico, la cual puede ser utilizada en diferentes problemáticas con variaciones específicas de acuerdo con el problema tratado o las características específicas del consultante.

  16. Global transcriptional landscape and promoter mapping of the gut commensal Bifidobacterium breve UCC2003.

    Science.gov (United States)

    Bottacini, Francesca; Zomer, Aldert; Milani, Christian; Ferrario, Chiara; Lugli, Gabriele Andrea; Egan, Muireann; Ventura, Marco; van Sinderen, Douwe

    2017-12-28

    Bifidobacterium breve represents a common member of the infant gut microbiota and its presence in the gut has been associated with host well being. For this reason it is relevant to investigate and understand the molecular mechanisms underlying the establishment, persistence and activities of this gut commensal in the host environment. The assessment of vegetative promoters in the bifidobacterial prototype Bifidobacterium breve UCC2003 was performed employing a combination of RNA tiling array analysis and cDNA sequencing. Canonical -10 (TATAAT) and -35 (TTGACA) sequences were identified upstream of transcribed genes or operons, where deviations from this consensus correspond to transcription level variations. A Random Forest analysis assigned the -10 region of B. breve promoters as the element most impacting on the level of transcription, followed by the spacer length and the 5'-UTR length of transcripts. Furthermore, our transcriptome study also identified rho-independent termination as the most common and effective termination signal of highly and moderately transcribed operons in B. breve. The present study allowed us to identify genes and operons that are actively transcribed in this organism during logarithmic growth, and link promoter elements with levels of transcription of essential genes in this organism. As homologs of many of our identified genes are present across the whole genus Bifidobacterium, our dataset constitutes a transcriptomic reference to be used for future investigations of gene expression in members of this genus.

  17. Bifidobacterium breve - HT-29 cell line interaction: modulation of TNF-a induced gene expression

    NARCIS (Netherlands)

    Boesten, R.J.; Vos, de W.M.; Schuren, F.H.J.; Willemsen, L.E.M.; Knol, J.

    2011-01-01

    To provide insight in the molecular basis for intestinal host-microbe interactions, we determined the genome-wide transcriptional response of human intestinal epithelial cells following exposure to cells of Bifidobacterium breve. To select an appropriate test system reflecting inflammatory

  18. A two-component regulatory system controls autoregulated serpin expression in Bifidobacterium breve UCC2003.

    Science.gov (United States)

    Alvarez-Martin, Pablo; O'Connell Motherway, Mary; Turroni, Francesca; Foroni, Elena; Ventura, Marco; van Sinderen, Douwe

    2012-10-01

    This work reports on the identification and molecular characterization of a two-component regulatory system (2CRS), encoded by serRK, which is believed to control the expression of the ser(2003) locus in Bifidobacterium breve UCC2003. The ser(2003) locus consists of two genes, Bbr_1319 (sagA) and Bbr_1320 (serU), which are predicted to encode a hypothetical membrane-associated protein and a serpin-like protein, respectively. The response regulator SerR was shown to bind to the promoter region of ser(2003), and the probable recognition sequence of SerR was determined by a combinatorial approach of in vitro site-directed mutagenesis coupled to transcriptional fusion and electrophoretic mobility shift assays (EMSAs). The importance of the serRK 2CRS in the response of B. breve to protease-mediated induction was confirmed by generating a B. breve serR insertion mutant, which was shown to exhibit altered ser(2003) transcriptional induction patterns compared to the parent strain, UCC2003. Interestingly, the analysis of a B. breve serU mutant revealed that the SerRK signaling pathway appears to include a SerU-dependent autoregulatory loop.

  19. Global transcriptional landscape and promoter mapping of the gut commensal Bifidobacterium breve UCC2003

    NARCIS (Netherlands)

    Bottacini, Francesca; Zomer, Aldert; Milani, Christian; Ferrario, Chiara; Lugli, Gabriele Andrea; Egan, Muireann; Ventura, Marco; van Sinderen, Douwe

    2017-01-01

    BACKGROUND: Bifidobacterium breve represents a common member of the infant gut microbiota and its presence in the gut has been associated with host well being. For this reason it is relevant to investigate and understand the molecular mechanisms underlying the establishment, persistence and

  20. Is Bifidobacterium breve effective in the treatment of childhood constipation? Results from a pilot study.

    Science.gov (United States)

    Tabbers, M M; de Milliano, I; Roseboom, M G; Benninga, M A

    2011-02-23

    Probiotics are increasingly used in the treatment of functional gastrointestinal disorders. Studies in constipated adults with a Bifidus yoghurt (containing Bifidobacterium breve, Bifidobacterium bifidum and Lactobacillus acidophilus) showed a significant increase in defecation frequency. The aim of this pilot study was to determine if Bifidobacterium breve is effective in the treatment of childhood constipation. Children, 3 to 16 years of age, with functional constipation according to the Rome III criteria were eligible for this study. During 4 weeks, children received one sachet of powder daily, containing 108- 1010 CFU Bifidobacterium breve. Furthermore, children were instructed to try to defecate on the toilet for 5-10 minutes after each meal and to complete a standardized bowel diary daily. The primary outcome measure was change in defecation frequency. Secondary outcome measures were stool consistency using the Bristol stool scale frequency of episodes of faecal incontinence, pain during defecation, frequency of abdominal pain, frequency of adverse effects (nausea, diarrhoea and bad taste), and frequency of intake of bisacodyl. Twenty children (75% male, mean age 7.4) were included in this pilot study. The defecation frequency per week significantly increased from 0.9 (0-2) at baseline to 4.9 (0-21) in week 4 (p Bifidobacterium breve is effective in increasing stool frequency in children with functional constipation. Furthermore it has a positive effect with respect to stool consistency, decreasing the number of faecal incontinence episodes and in diminishing abdominal pain. A randomized placebo controlled trial is required to confirm these data.

  1. Discovering novel bile protection systems in Bifidobacterium breve UCC2003 through functional genomics.

    NARCIS (Netherlands)

    Ruiz, L.; Zomer, A.L.; O'Connell-Motherway, M.; Sinderen, D. van; Margolles, A.

    2012-01-01

    Tolerance of gut commensals to bile salt exposure is an important feature for their survival in and colonization of the intestinal environment. A transcriptomic approach was employed to study the response of Bifidobacterium breve UCC2003 to bile, allowing the identification of a number of

  2. The effects of Bifidobacterium breve on immune mediators and proteome of HT29 cells monolayers.

    Science.gov (United States)

    Sánchez, Borja; González-Rodríguez, Irene; Arboleya, Silvia; López, Patricia; Suárez, Ana; Ruas-Madiedo, Patricia; Margolles, Abelardo; Gueimonde, Miguel

    2015-01-01

    The use of beneficial microorganisms, the so-called probiotics, to improve human health is gaining popularity. However, not all of the probiotic strains trigger the same responses and they differ in their interaction with the host. In spite of the limited knowledge on mechanisms of action some of the probiotic effects seem to be exerted through maintenance of the gastrointestinal barrier function and modulation of the immune system. In the present work, we have addressed in vitro the response of the intestinal epithelial cell line HT29 to the strain Bifidobacterium breve IPLA20004. In the array of 84 genes involved in inflammation tested, the expression of 12 was modified by the bifidobacteria. The genes of chemokine CXCL6, the chemokine receptor CCR7, and, specially, the complement component C3 were upregulated. Indeed, HT29 cells cocultivated with B. breve produced significantly higher levels of protein C3a. The proteome of HT29 cells showed increased levels of cytokeratin-8 in the presence of B. breve. Altogether, it seems that B. breve IPLA20004 could favor the recruitment of innate immune cells to the mucosa reinforcing, as well as the physical barrier of the intestinal epithelium.

  3. Digestion of Human Milk Oligosaccharides by Bifidobacterium breve in the Premature Infant.

    Science.gov (United States)

    Underwood, Mark A; Davis, Jasmine C C; Kalanetra, Karen M; Gehlot, Sanjay; Patole, Sanjay; Tancredi, Daniel J; Mills, David A; Lebrilla, Carlito B; Simmer, Karen

    2017-10-01

    The aim of this study was to measure consumption and absorption of human milk oligosaccharides (HMOs) in a cohort of premature infants treated with probiotic Bifidobacterium breve. Twenty-nine premature infants (median gestational age 28 weeks, range 23-32 weeks) cared for in the neonatal intensive care unit of the King Edward and Princess Margaret Hospital in Perth, Australia, were treated with B breve at a dose of 1.66 billion organisms per day. Samples of feces, urine, and milk were obtained at initiation of the probiotic and again 3 weeks later. 16S ribosomal RNA from the feces was analyzed by next-generation sequencing. Quantitation of HMO content of the milk, urine, and feces was performed using nano-high-performance liquid chromatography-chip/time-of-flight mass spectrometry. There was heterogeneity in colonization with bifidobacteria. "Responders" received milk with higher percentages of fucosylated HMOs and had higher percentages of bifidobacteria and lower percentages of Enterobacteriaceae in their feces than "nonresponders." Several individual HMOs in the milk were associated with changes in fecal bifidobacteria over time. Changes over time in milk, fecal, and urine HMOs suggested heterogeneity among HMO structures in consumption by microbes in the gut lumen and absorption from the intestine. Colonization of the premature infant intestinal tract with probiotic B breve is influenced by prebiotic HMOs. B breve is a selective consumer of HMOs in the premature infant.

  4. Bifidobacterium breve-HT-29 cell line interaction: Modulation of TNF-a induced gene expression

    NARCIS (Netherlands)

    Boesten, R.J.; Schuren, F.H.J.; Willemsen, L.E.M.; Vriesema, A.; Knol, J.; Vos, W.M. de

    2011-01-01

    To provide insight in the molecular basis for intestinal host-microbe interactions, we determined the genome-wide transcriptional response of human intestinal epithelial cells following exposure to cells of Bifidobacterium breve. To select an appropriate test system reflecting inflammatory

  5. The Effects of Bifidobacterium breve on Immune Mediators and Proteome of HT29 Cells Monolayers

    Directory of Open Access Journals (Sweden)

    Borja Sánchez

    2015-01-01

    Full Text Available The use of beneficial microorganisms, the so-called probiotics, to improve human health is gaining popularity. However, not all of the probiotic strains trigger the same responses and they differ in their interaction with the host. In spite of the limited knowledge on mechanisms of action some of the probiotic effects seem to be exerted through maintenance of the gastrointestinal barrier function and modulation of the immune system. In the present work, we have addressed in vitro the response of the intestinal epithelial cell line HT29 to the strain Bifidobacterium breve IPLA20004. In the array of 84 genes involved in inflammation tested, the expression of 12 was modified by the bifidobacteria. The genes of chemokine CXCL6, the chemokine receptor CCR7, and, specially, the complement component C3 were upregulated. Indeed, HT29 cells cocultivated with B. breve produced significantly higher levels of protein C3a. The proteome of HT29 cells showed increased levels of cytokeratin-8 in the presence of B. breve. Altogether, it seems that B. breve IPLA20004 could favor the recruitment of innate immune cells to the mucosa reinforcing, as well as the physical barrier of the intestinal epithelium.

  6. La terapia breve: una alternativa de intervención psicológica efectiva

    Directory of Open Access Journals (Sweden)

    Carlos Andrés Gantiva Díaz

    2009-06-01

    Full Text Available El objetivo de este trabajo es describir y estructurar los orígenes, bases teóricas y los procedimientos de la terapia breve desde la perspectiva terapéutica cognitivo conductual. Se inicia con la identifi cación de las bases teóricas de la terapia breve, principalmente las teorías motivacionales relacionadas con la disponibilidad al cambio y la autoefi cacia desde la teoría social cognitiva. Se realiza un paralelo entre las características principales de la terapia breve en contraposición con las terapias tradicionales. Por último, se propone una estructura procedimental general de estrategias clínicas a emplear en la terapia breve con soporte empírico, la cual puede ser utilizada en diferentes problemáticas con variaciones específi cas de acuerdo con el problema tratado o las características específi cas del consultante

  7. [Toxic effects of nano-TiO2 on Gymnodinium breve].

    Science.gov (United States)

    Li, Feng-Min; Zhao, Wei; Li, Yuan-Yuan; Tian, Zhi-Jia; Wang, Zhen-Yu

    2012-01-01

    In order to reveal the toxicity and mechanism of nano-TiO2 on algae, the inhibition effect, enzyme activity, oxygen free radicals of nano-TiO2 on the growth of G. breve were investigated. The results showed that G. breve was inhibited by nano-TiO2, and the 72 h-EC50 was 9.7 mg x L(-1). With the increasing concentration of nano-titanium dioxide, the activities of SOD decrease significantly (P TiO2 suspension while that was 1.1 U x mL(-1) in control after 48 h. Through the study of 20 mg x L(-1) nano-titanium dioxide on G. breve at different times, the activities of SOD and CAT, the content of MDA are consistent, which the highest values is achieved at the exposure time of 12 hours and the lowest value is found at the exposure time of 48 hours. The content of hydroxyl radical increased significantly at the exposure time of 48 hours. The activity of SOD was 0.14 U x (10(7) cell x min)(-1) in G. breve at 12 h which was ten times higher than that at 48 h.

  8. Satellite growth of Legionella pneumophila with an environmental isolate of Flavobacterium breve.

    Science.gov (United States)

    Wadowsky, R M; Yee, R B

    1983-12-01

    Legionella pneumophila serogroup 1 was observed to satellite around colonies of Flavobacterium breve on an L-cysteine-deficient medium which did not support growth of legionellae. Both isolates were recovered from the hot water tanks of hospitals. Ferric PPi stimulated satellite growth between 0.01 and 0.1%.

  9. Satellite growth of Legionella pneumophila with an environmental isolate of Flavobacterium breve.

    OpenAIRE

    Wadowsky, R M; Yee, R B

    1983-01-01

    Legionella pneumophila serogroup 1 was observed to satellite around colonies of Flavobacterium breve on an L-cysteine-deficient medium which did not support growth of legionellae. Both isolates were recovered from the hot water tanks of hospitals. Ferric PPi stimulated satellite growth between 0.01 and 0.1%.

  10. Effects of Bifidobacterium breve on inflammatory gene expression in neonatal and weaning rat intestine.

    Science.gov (United States)

    Ohtsuka, Yoshikazu; Ikegami, Takako; Izumi, Hirohisa; Namura, Mariko; Ikeda, Tomomi; Ikuse, Tamaki; Baba, Yosuke; Kudo, Takahiro; Suzuki, Ryuyo; Shimizu, Toshiaki

    2012-01-01

    To examine the immune-modulatory effects of probiotics during early infancy, Bifidobacterium breve M-16V (B. breve) was administered to rat pups during the newborn or weaning period, and the expression of inflammatory genes was investigated using a cDNA microarray and real-time PCR. After B. breve administration, significant increases in the numbers of Bifidobacterium in both the cecum and colon were confirmed during the newborn period. The numbers of upregulated and downregulated genes were greater during the weaning period than in the newborn period and were greatest in the colon, with fewer genes altered in the small intestine and the fewest in the spleen. The expression of inflammation-related genes, including lipoprotein lipase (Lpl), glutathione peroxidase 2 (Gpx2), and lipopolysaccharide-binding protein (Lbp), was significantly reduced in the colon during the newborn period. In weaning rat pups, the expression of CD3d, a cell surface receptor-linked signaling molecule, was significantly enhanced in the colon; however, the expression of co-stimulatory molecules was not enhanced. Our findings support a possible role for B. breve in mediating anti-inflammatory and antiallergic reactions by modulating the expression of inflammatory molecules during the newborn period and by regulating the expression of co-stimulatory molecules during the weaning period. Gene expression in the intestine was investigated after feeding 5 × 10(8) cfu of B. breve every day to the F344/Du rat from days 1 to 14 (newborn group) and from days 21 to 34 (weaning group). mRNA was extracted from intestine, and the expression of inflammatory gene was analyzed by microarray and real-time PCR.

  11. La emigración como proceso de duelo. Protocolo de psicoterapia breve centrada en su elaboración.

    OpenAIRE

    Isabel Salvador Sánchez

    2002-01-01

    Se presenta un protocolo de psicoterapia breve para el tratamiento de pacientes emigrantes con el diagnóstico psicodinámico de “duelo no elaborado”, explicando las características de dicho protocolo.

  12. Pre-treatment with Bifidobacterium breve UCC2003 modulates Citrobacter rodentium-induced colonic inflammation and organ specificity.

    Science.gov (United States)

    Collins, James W; Akin, Ali R; Kosta, Artemis; Zhang, Ning; Tangney, Mark; Francis, Kevin P; Frankel, Gad

    2012-11-01

    Citrobacter rodentium, which colonizes the gut mucosa via formation of attaching and effacing (A/E) lesions, causes transmissible colonic hyperplasia. The aim of this study was to evaluate whether prophylactic treatment with Bifidobacterium breve UCC2003 can improve the outcome of C. rodentium infection. Six-week-old albino C57BL/6 mice were pre-treated for 3 days with B. breve, challenged with bioluminescent C. rodentium and administered B. breve or PBS-C for 8 days post-infection; control mice were either administered B. breve and mock-infected with PBS, or mock-treated with PBS-C and mock-infected with PBS. C. rodentium colonization was monitored by bacterial enumeration from faeces and by a combination of both 2D bioluminescence imaging (BLI) and composite 3D diffuse light imaging tomography with µCT imaging (DLIT-µCT). At day 8 post-infection, colons were removed and assessed for crypt hyperplasia, histology by light microscopy, bacterial colonization by immunofluorescence, and A/E lesion formation by electron microscopy. Prophylactic administration of B. breve did not prevent C. rodentium colonization or A/E lesion formation. However, this treatment did alter C. rodentium distribution within the large intestine and significantly reduced colonic crypt hyperplasia at the peak of bacterial infection. These results show that B. breve could not competitively exclude C. rodentium, but reduced pathogen-induced colonic inflammation.

  13. Efficacy of Bifidobacterium breve NCC2950 against DSS-induced colitis is dependent on bacterial preparation and timing of administration.

    Science.gov (United States)

    Hayes, C L; Natividad, J M M; Jury, J; Martin, R; Langella, P; Verdu, E F

    2014-03-01

    Probiotics have been proposed as a therapy for inflammatory bowel disease, but variations in strains, formulations, and protocols used in clinical trials have hindered the creation of guidelines for their use. Thus, preclinical insight into the mechanisms of specific probiotic strains and mode of administration would be useful to guide future clinical trial design. In this study, live, heat inactivated (HI), and spent culture medium preparations of the probiotic Bifidobacterium breve NCC2950 were administered to specific pathogen free C57BL/6 mice before or during colitis, as well as before colitis reactivation. Five days of 3.5% dextran sulphate sodium in drinking water was used to induce colitis. Pretreatment with live B. breve reduced disease severity, myeloperoxidase activity, microscopic damage, cytokine production, interleukin (IL)-12/IL-10 ratio, and lymphocyte infiltration in the colon. B. breve did not attenuate on-going colitis. After acute colitis, disease symptoms were normalised sooner with live and HI B. breve treatment; however, reactivation of colitis was not prevented. These findings indicate that the efficacy of a probiotic to modulate intestinal inflammation is dependent on the formulation as well as state of inflammation when administered. Overall, live B. breve was most efficacious in preventing acute colitis. Live and HI B. breve also promoted recovery from diarrhoea and colon bleeding after a bout of acute colitis.

  14. Is Bifidobacterium breve effective in the treatment of childhood constipation? Results from a pilot study

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    Roseboom MG

    2011-02-01

    Full Text Available Abstract Background Probiotics are increasingly used in the treatment of functional gastrointestinal disorders. Studies in constipated adults with a Bifidus yoghurt (containing Bifidobacterium breve, Bifidobacterium bifidum and Lactobacillus acidophilus showed a significant increase in defecation frequency. The aim of this pilot study was to determine if Bifidobacterium breve is effective in the treatment of childhood constipation. Methods Children, 3 to 16 years of age, with functional constipation according to the Rome III criteria were eligible for this study. During 4 weeks, children received one sachet of powder daily, containing 108- 1010 CFU Bifidobacterium breve. Furthermore, children were instructed to try to defecate on the toilet for 5-10 minutes after each meal and to complete a standardized bowel diary daily. The primary outcome measure was change in defecation frequency. Secondary outcome measures were stool consistency using the Bristol stool scale frequency of episodes of faecal incontinence, pain during defecation, frequency of abdominal pain, frequency of adverse effects (nausea, diarrhoea and bad taste, and frequency of intake of bisacodyl. Results Twenty children (75% male, mean age 7.4 were included in this pilot study. The defecation frequency per week significantly increased from 0.9 (0-2 at baseline to 4.9 (0-21 in week 4 (p Conclusion Bifidobacterium breve is effective in increasing stool frequency in children with functional constipation. Furthermore it has a positive effect with respect to stool consistency, decreasing the number of faecal incontinence episodes and in diminishing abdominal pain. A randomized placebo controlled trial is required to confirm these data.

  15. Recordando a Erlang : un breve paseo (sin esperas) por la Teoría de Colas

    OpenAIRE

    Delgado de la Torre, Rosario

    2009-01-01

    Este artículo tiene un doble objetivo : por una parte, dar cuenta de la celebración del centenario de la Teoría de Colas ; por otra, presentar una breve introducción a lo más clásico de dicha teoría, que pueda resultar de utilidad para aquellos que teniendo unos conocimientos elementales de probabilidad desean introducirse en ella de manera rápida y sin demasiadas complicaciones.

  16. The probiotic Bifidobacterium breve B632 inhibited the growth of Enterobacteriaceae within colicky infant microbiota cultures.

    Science.gov (United States)

    Simone, Marta; Gozzoli, Caterina; Quartieri, Andrea; Mazzola, Giuseppe; Di Gioia, Diana; Amaretti, Alberto; Raimondi, Stefano; Rossi, Maddalena

    2014-01-01

    Infant colic is a common gastrointestinal disorder of newborns, mostly related to imbalances in the composition of gut microbiota and particularly to the presence of gas-producing coliforms and to lower levels of Bifidobacteria and Lactobacilli. Probiotics could help to contain this disturbance, with formulations consisting of Lactobacillus strains being the most utilized. In this work, the probiotic strain Bifidobacterium breve B632 that was specifically selected for its ability to inhibit gas-producing coliforms, was challenged against the Enterobacteriaceae within continuous cultures of microbiota from a 2-month-old colicky infant. As confirmed by RAPD-PCR fingerprinting, B. breve B632 persisted in probiotic-supplemented microbiota cultures, accounting for the 64% of Bifidobacteria at the steady state. The probiotic succeeded in inhibiting coliforms, since FISH and qPCR revealed that the amount of Enterobacteriaceae after 18 h of cultivation was 0.42 and 0.44 magnitude orders lower (P breve B632 to a cohort of colicky newborns, in order to observe the behavior of this strain in vivo and to validate its effect in colic treatment.

  17. Metabolism of a plant derived galactose‐containing polysaccharide by Bifidobacterium breve UCC2003

    Science.gov (United States)

    O'Connell Motherway, Mary; Fitzgerald, Gerald F.; van Sinderen, Douwe

    2011-01-01

    Summary In this study, we describe the functional characterization of the Bifidobacterium breve UCC2003 gal locus, which is dedicated to the utilization of galactan, a plant‐derived polysaccharide. Using a combination of molecular approaches we conclude that the galA gene of B. breve UCC2003 encodes a β‐1,4‐endogalactanase producing galacto‐oligosaccharides, which are specifically internalized by an ABC transport system, encoded by galBCDE, and which are then hydrolysed to galactose moieties by a dedicated intracellular β‐galactosidase, specified by galG. The generated galactose molecules are presumed to be fed into the fructose‐6‐phosphate phosphoketolase pathway via the Leloir pathway, thereby allowing B. breve UCC2003 to use galactan as its sole carbon and energy source. In addition to these findings we demonstrate that GalR is a LacI‐type DNA‐binding protein, which not only appears to control transcription of the galCDEGR operon, but also that of the galA gene. PMID:21375716

  18. A GntR-type transcriptional repressor controls sialic acid utilization in Bifidobacterium breve UCC2003.

    Science.gov (United States)

    Egan, Muireann; O'Connell Motherway, Mary; van Sinderen, Douwe

    2015-02-01

    Bifidobacterium breve strains are numerically prevalent among the gut microbiota of healthy, breast-fed infants. The metabolism of sialic acid, a ubiquitous monosaccharide in the infant and adult gut, by B. breve UCC2003 is dependent on a large gene cluster, designated the nan/nag cluster. This study describes the transcriptional regulation of the nan/nag cluster and thus sialic acid metabolism in B. breve UCC2003. Insertion mutagenesis and transcriptome analysis revealed that the nan/nag cluster is regulated by a GntR family transcriptional repressor, designated NanR. Crude cell extract of Escherichia coli EC101 in which the nanR gene had been cloned and overexpressed was shown to bind to two promoter regions within this cluster, each of which containing an imperfect inverted repeat that is believed to act as the NanR operator sequence. Formation of the DNA-NanR complex is prevented in the presence of sialic acid, which we had previously shown to induce transcription of this gene cluster. © FEMS 2014. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.

  19. Preclinical Immunomodulation by the Probiotic Bifidobacterium breve M-16V in Early Life.

    Science.gov (United States)

    Rigo-Adrover, Maria Del Mar; Franch, Àngels; Castell, Margarida; Pérez-Cano, Francisco José

    2016-01-01

    This study aimed to investigate the effect of supplementation with the probiotic Bifidobacterium breve M-16V on the maturation of the intestinal and circulating immune system during suckling. In order to achieve this purpose, neonatal Lewis rats were supplemented with the probiotic strain from the 6th to the 18th day of life. The animals were weighed during the study, and faecal samples were obtained and evaluated daily. On day 19, rats were euthanized and intestinal wash samples, mesenteric lymph node (MLN) cells, splenocytes and intraepithelial lymphocytes (IEL) were obtained. The probiotic supplementation in early life did not modify the growth curve and did not enhance the systemic immune maturation. However, it increased the proportion of cells bearing TLR4 in the MLN and IEL, and enhanced the percentage of the integrin αEβ7+ and CD62L+ cells in the MLN and that of the integrin αEβ7+ cells in the IEL, suggesting an enhancement of the homing process of naïve T lymphocytes to the MLN, and the retention of activated lymphocytes in the intraepithelial compartment. Interestingly, B. breve M-16V enhanced the intestinal IgA synthesis. In conclusion, supplementation with the probiotic strain B. breve M-16V during suckling improves the development of mucosal immunity in early life.

  20. Metabolism of a plant derived galactose-containing polysaccharide by Bifidobacterium breve UCC2003.

    Science.gov (United States)

    O'Connell Motherway, Mary; Fitzgerald, Gerald F; van Sinderen, Douwe

    2011-05-01

    In this study, we describe the functional characterization of the Bifidobacterium breve UCC2003 gal locus, which is dedicated to the utilization of galactan, a plant-derived polysaccharide. Using a combination of molecular approaches we conclude that the galA gene of B. breve UCC2003 encodes a β-1,4-endogalactanase producing galacto-oligosaccharides, which are specifically internalized by an ABC transport system, encoded by galBCDE, and which are then hydrolysed to galactose moieties by a dedicated intracellular β-galactosidase, specified by galG. The generated galactose molecules are presumed to be fed into the fructose-6-phosphate phosphoketolase pathway via the Leloir pathway, thereby allowing B. breve UCC2003 to use galactan as its sole carbon and energy source. In addition to these findings we demonstrate that GalR is a LacI-type DNA-binding protein, which not only appears to control transcription of the galCDEGR operon, but also that of the galA gene. © 2010 University College Cork. Journal compilation © 2010 Society for Applied Microbiology and Blackwell Publishing Ltd.

  1. The Probiotic Bifidobacterium breve B632 Inhibited the Growth of Enterobacteriaceae within Colicky Infant Microbiota Cultures

    Directory of Open Access Journals (Sweden)

    Marta Simone

    2014-01-01

    Full Text Available Infant colic is a common gastrointestinal disorder of newborns, mostly related to imbalances in the composition of gut microbiota and particularly to the presence of gas-producing coliforms and to lower levels of Bifidobacteria and Lactobacilli. Probiotics could help to contain this disturbance, with formulations consisting of Lactobacillus strains being the most utilized. In this work, the probiotic strain Bifidobacterium breve B632 that was specifically selected for its ability to inhibit gas-producing coliforms, was challenged against the Enterobacteriaceae within continuous cultures of microbiota from a 2-month-old colicky infant. As confirmed by RAPD-PCR fingerprinting, B. breve B632 persisted in probiotic-supplemented microbiota cultures, accounting for the 64% of Bifidobacteria at the steady state. The probiotic succeeded in inhibiting coliforms, since FISH and qPCR revealed that the amount of Enterobacteriaceae after 18 h of cultivation was 0.42 and 0.44 magnitude orders lower (P<0.05 in probiotic-supplemented microbiota cultures than in the control ones. These results support the possibility to move to another level of study, that is, the administration of B. breve B632 to a cohort of colicky newborns, in order to observe the behavior of this strain in vivo and to validate its effect in colic treatment.

  2. BDNF expression in the hippocampus of maternally separated rats: does Bifidobacterium breve 6330 alter BDNF levels?

    Science.gov (United States)

    O'Sullivan, E; Barrett, E; Grenham, S; Fitzgerald, P; Stanton, C; Ross, R P; Quigley, E M M; Cryan, J F; Dinan, T G

    2011-09-01

    Brain-derived neurotrophic factor (BDNF) is of interest because of its putative role in stress and psychiatric disorders. Maternal separation is used as an animal model of early-life stress and of irritable bowel syndrome (IBS). Animals exposed to the paradigm show altered gut function together with heightened levels of arousal and corticosterone. Some probiotic organisms have been shown to be of benefit in IBS and influence the brain-gut axis. Our objective was to investigate the effects of maternal separation on BDNF under basal conditions and in response to the probiotic Bifidobacterium breve 6330. The study implemented the maternal separation model which we have previously described. Polymerase chain reaction and in situ hybridisation were performed to measure the effect of maternal separation on both BDNF total variants and BDNF splice variant (exon) IV in the hippocampus. Maternally separated and non-separated rats were treated with B. breve 6330, to investigate the effect of this probiotic on BDNF total variant and BDNF exon IV expression. Maternal separation increased BDNF total variants (Pbreve 6330 increased BDNF total variants (Pbreve 6330 did not alter BDNF levels in the maternally separated rats. Maternal separation caused a marked increase in BDNF in the hippocampus. While B. breve 6330 influenced BDNF in normal animals, it had no significant effect on BDNF in those which were maternally separated. We have demonstrated that an orally administered probiotic can influence hippocampal BDNF.

  3. Preclinical Immunomodulation by the Probiotic Bifidobacterium breve M-16V in Early Life.

    Directory of Open Access Journals (Sweden)

    Maria Del Mar Rigo-Adrover

    Full Text Available This study aimed to investigate the effect of supplementation with the probiotic Bifidobacterium breve M-16V on the maturation of the intestinal and circulating immune system during suckling. In order to achieve this purpose, neonatal Lewis rats were supplemented with the probiotic strain from the 6th to the 18th day of life. The animals were weighed during the study, and faecal samples were obtained and evaluated daily. On day 19, rats were euthanized and intestinal wash samples, mesenteric lymph node (MLN cells, splenocytes and intraepithelial lymphocytes (IEL were obtained. The probiotic supplementation in early life did not modify the growth curve and did not enhance the systemic immune maturation. However, it increased the proportion of cells bearing TLR4 in the MLN and IEL, and enhanced the percentage of the integrin αEβ7+ and CD62L+ cells in the MLN and that of the integrin αEβ7+ cells in the IEL, suggesting an enhancement of the homing process of naïve T lymphocytes to the MLN, and the retention of activated lymphocytes in the intraepithelial compartment. Interestingly, B. breve M-16V enhanced the intestinal IgA synthesis. In conclusion, supplementation with the probiotic strain B. breve M-16V during suckling improves the development of mucosal immunity in early life.

  4. Effect of temperature on the proliferation of Gymnodinium breve and Gomphosphaeria aponina

    Energy Technology Data Exchange (ETDEWEB)

    Eng-Wilmot, D.L.; Hitchcock, W.S.; Martin, D.F.

    1977-01-01

    The effect of temperature on the growth and proliferation of two marine microorganisms, the toxigenic dinoflagellate Gymnodinium breve, and a potential bio-control organism, the blue-green alga Gomphosphaeria aponina, was determined by culturing the organisms in thermal gradients established by heating and cooling the opposite ends of an aluminum bar that had been adapted to hold culture tubes. Gradients were linear and stable for the duration of each trial. There was no relationship between variations in light and growth of the organisms. Gymnodinium breve showed optimum growth at 22/sup 0/C, and prolifereated over a range of temperatures (17/sup 0/ to 30/sup 0/C). Below 17/sup 0/C cultures of G. breve declined in growth, and at 4/sup 0/C the organisms died within 5 h. Above 31/sup 0/C there was rapid decline in viability of cells, and at 33.5/sup 0/C the organism died within 24 h. Gomphosphaeria aponina showed optimum growth between 24/sup 0/ and 29/sup 0/C, with a maximum at 27/sup 0/C. Growth at temperatures greater than 31/sup 0/C was minimal, but the organism survived. Limitation may be due to repression of the bio-synthesis of an iron-transport compound.

  5. Breves Notícias Sobre a Geologia dos Estados do Paraná e Santa Catarina

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    Maack Reinhard

    2001-01-01

    Full Text Available The present short survey of the geology of the States of Paraná and Santa Catarina was written as a text to accompany sketches for the revision of the GEOLOGICAL MAP OF SOUTH AMERICA. It contains the results of geologic research up to the year 1947 in most concise form, without going into detail. As requested, the occurrence of useful minerals was not considered in the summary, and the list of bibliographical references has been condensed to the uttermost. Inasmuch as the author treated the Passa Dois Series in more detail, due to the reorganization of the Gondwana beds, the Archean and the Algonkian had to be reduced to the minimum. This was possible, since the innumerable details of the phenomena in the crystalline complex could not be represented in a geologic map of the given scale. The reorganization of the geologic constituents of Paraná and Santa Catarina is summarized in Stratigraphic Table nº 2, and is explained by the enclosed sketches.

  6. El cuerpo y la salida del ser. Breve apunte sobre el problema de la subjetividad y la responsabilidad en Levinas

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    Fernando Escobar Díaz

    2014-01-01

    Full Text Available Se ofrece una posible lectura de la trascendencia levinasiana desde el fenómeno de la responsabilidad, lectura que no es posible desde la lógica de la libertad, sino en cuanto que trascendencia del retorno sin partir de ninguna parte. Esto exige aceptar que la sensibilidad es el punto cero de la situación, con lo que se pone de manifiesto que el hombre, antes de trascender y responder, está encarnado. Por lo tanto, la corporalidad del cuerpo es el hecho desde el cual un sujeto responsable es posible.

  7. Vibraciones digitales. Una breve historia sobre los recursos electrónicos y digitales en la música

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    Pau Damià Riera Muñoz

    2012-05-01

    Full Text Available In the last two decades, the music consumption habits have changed dramatically thanks to new technologies. Digital media recording and playback, with the Internet, have allowed everybody the access to a huge musical repertoire. On the other hand, music creation tools are changing very quickly, especially since in the early eighties musicians started to use MIDI programming standards. The use and abuse of musical creation and performance is growing thanks to digitization. What consequences bring these changes towards the public? And how these changes affect the industry professionals?

  8. Breves notas sobre A paz perpétua: ética, política e devir

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    Antonio Basílio Novaes Thomaz de Menezes

    2009-06-01

    Full Text Available This paper is defined first and foremost a mental experiment: read Peace Perpetua with the lenses of Foucaultian problematization of power in terms of a critical ontology of the present. Towards a characterization of the critical current in the analysis of the framework of historical deconstruction of reason as a guarantor of peace and universal as the ultimate Enlightenment project, this test is to rebuild the theme object Peace Perpetua, what also makes it possible today from the perspective of power relations. His object contrasts the idea of a design philosophy with the task of an incentive to challenge the letter Kantian to think outside their controlled environment, by transposing the barriers of reading the task of thinking the possibility of peace in the contemporary world.

  9. Dualidades luteranas. Breve ensayo sobre la libertad, la servidumbre y la obediencia en Martín Lutero

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    Ignacio Luis Moretti

    2016-06-01

    Full Text Available ¿Cómo es posible pensar la relación entre los preceptos religiosos y los conceptos políticos de igualdad, libertad y ley? ¿Se trata de esferas mutuamente excluyentes o de dualidades en permanente tensión? ¿Cómo compatibilizar la obediencia religiosa con la primacía de la ley positiva? Estos son algunos de los interrogantes que pretende exponer la inherente tirantez constituyente de todo pensamiento político en relación con la sustancia y el papel de la religión, y su consecuente influencia en el tenor, densidad y caracterización de la política y lo político. Lejos de constituirse en una relación simple o lineal para el pensamiento político, plantea diversas problemáticas que deben ser explicitadas y analizadas. Este pequeño ensayo pretende esbozar un análisis crítico de esta relación, tomando para ello el pensamiento rupturista de Martín Lutero y complementando sus reformas teológicas con su pensamiento eminentemente político.

  10. Let vibrate: Um breve panorama sobre o vibrafone na música do século XX

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    Fernando Chaib

    2008-05-01

    Full Text Available This article surveys the origins and development of the vibraphone and its repertory in twentieth century concert music. After tracing briefly the history of the instrument itself, I will consider how the works of some composers were not only crucial in broadening the vibraphone's repertoire, but were also helpful in fostering new aesthetic thoughts in musical composition. The present article also aims at expanding the scarce bibliography on the vibraphone in Portuguese language.

  11. Breves notas sobre A paz perpétua: ética, polí­tica e devir

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    Antonio Basílio Novaes Thomaz Menezes

    2010-09-01

    Full Text Available Este ensaio se define antes de tudo por um experimento mental: ler A Paz Perpétua com as lentes foucaultianas da problematizaçáo do poder, nos termos de uma “ontologia crítica do presente”. Voltado para uma caracterizaçáo crítica da atualidade na análise da do quadro de desconstruçáo histórica da razáo como avalista da paz e do universal como fim último do projeto iluminista, este ensaio tem por objeto reconstruir o tema da Paz Perpétua, naquilo que ainda o torna possível hoje sob a ótica das relações de poder. Seu objeto contrasta a ideia de um projeto filosófico com a tarefa de um incitamento ao desafio de pensar a letra kantiana fora do seu quadro restrito, transpondo as barreiras da leitura a tarefa de pensar a possibilidade da Paz no mundo contemporâneo.

  12. Duas epígrafes e uma breve reflexão sobre o valor biográfico

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    Diana Klinger

    2013-06-01

    Full Text Available http://dx.doi.org/10.5007/2176-8552.2012n14p23 O texto propõe intervir no debate em torno da relevância do valorbiográfico na leitura da literatura contemporânea. Longe do paradigma daautonomia do literário, a partir do qual Roland Barthes enunciava a “mortedo autor”, a circulação cada vez maior da ideia de pós-autonomia abre a possibilidadede ler conjuntamente o texto e seu exterior, ou seja, a “realidade”ou “a própria vida”. Por outro lado, pode-se argumentar, como faz BernardoCarvalho, que essa excessiva atenção ao autor (quase uma santificação levaà “morte da obra”. Como fazer uma leitura do sujeito da escrita que nãoredunde em narcisismo e espetacularização? Alguns poemas de Carlito Azevendosão o ponto de partida para pensar o autor como uma “sobrevivência”ou uma figura intermitente, no sentido que esse conceito tem para Didi--Huberman em A sobrevivência dos vagalumes, re-significando o biográficocomo espaço de abertura e encontro com o outro.

  13. Breve reflexión sobre la música electroacústica en México

    OpenAIRE

    Sigal, Rodrigo

    2010-01-01

    México tiene una herencia musical excepcional, en gran medida resultado de la influencia de una mezcla de antiguas tradiciones musicales europeas e indígenas mexicanas, y las tendencias contemporáneas siguen reflejando una gran diversidad de estilos nacionales y regionales. La adaptación de enfoques musicales de Europa empezó durante el periodo colonial, en los siglos XVI y XVII, y continúa hasta el presente. Entre las incipientes innovaciones musicales de los últimos sesenta años en Europa y...

  14. CARACTERÍSTICA DOS SEGMENTOS SETORIAIS NO BRASIL: UMA BREVE ABORDAGEM SOBRE O SETOR DA AGROINDÚSTRIA

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    Diogo Del Fiori

    2014-07-01

    Full Text Available O presente trabalho se propõe a fazer uma análise das características setoriais do Brasil desde a segunda metade do século XIX até a data presente, com o objetivo de mostrar que embora tenham ocorrido intervenções do Estado para a realização do processo de industrialização ao longo da história brasileira, nota-se que elas foram de certa forma ineficiente, e acabaram influenciando a trajetória dos setores econômicos de tal forma que na data presente, o Brasil possui a predominância de exportação dos setores básicos, sendo uma verdadeira contradição para as metas que o Brasil almeja alcançar nas próximas décadas. Este trabalho sugere que o Brasil necessita rever suas políticas e escolher algum setor líder no mercado mundial e o setor da Agroindústria poderia ser o segmento econômico líder do país, conforme será abordado neste trabalho. Palavras-Chave: Indústrias, Estado, Setores Abstract: This work intends to make an analysis of industry characteristics in Brazil since the mid-nineteenth century to the present date in order to show that although there were state interventions to the achievement of industrialization throughout history Brazilian note that they were somewhat inefficient, and ultimately influencing the trajectory of economic sectors so that at the present time, Brazil has the predominance of the basic sectors of export, being a real contradiction to the goals that Brazil intends to reach the coming decades. This work suggests that Brazil needs to review its policies and choose a sector leader in the world market and agribusiness sector could be the key economic sector in Brazil, according to his approach in this work. Key Words: Industries, State, Sectors

  15. Breve síntesis sobre el armamento en Egipto durante las dinastías XIX y XX

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    Javier Martínez Babón

    2005-01-01

    Full Text Available Los ejércitos de Seti y de Ramses II que lucharon contra los hititas estaban en posesión de la mayoría de las armas más modernas de su época. Las tropas egipcias dependían de los carros y de la infantería que esencialmente luchaba con hachas, espadas cortas, espadas de hoz, jabalinas, mazas, venablos, hondas y se defendían con escudos. El uso de cascos, cotas de placas de bronce se extendió con rapidez y los mercenarios shardana introdujeron las espadas largas. Con la crisis política a finales de la dinastía XIX, y la pérdida de territorios a inicios de la dinastía del XX, el ejército perdió también parte de sus recursos y los artesanos asiáticos especializados en la metalurgia.The army of Seti I and Ramses II, which fought against the hittites, possessed the most modem weapons of that era. The Egyptian troops depended upon chariots and infantry which essentially fought with axes, short swords, sickle swords, spears, javelins, lances, self and composite bows and who defended themselves with shields. The use of helmets and coats of mail became more and more widespread and the shardana mercenaries introduced long swords. With the political crisis at the end of the XIXth. Dynasty and the loss of territory at the beginning of the XXth. Dynasty, the army lost its arms resources, which come from Asian towns specialized in metallurgy.

  16. Breve estudio sobre las Patentes y su utilización como fuente de Información.

    Directory of Open Access Journals (Sweden)

    Liset Pentón Martínez

    2007-01-01

    Full Text Available El desarrollo acelerado de la producción demanda constantemente de nuevas técnicas, vinculadas directamente al avance de la ciencia y la tecnología, como respuesta del hombre al desafío de la vida, y dentro de ellas las innovaciones, como frutos específicos, tienen gran importancia, por lo que se hace necesario dejar bien claro que no basta con generar ideas, sino que han de ser protegidas, así como a los creadores de estas ideas, que también merecen encontrar protección a sus derechos, considerando de vital importancia realizar una revisión bibliográfica, tratando de familiarizar al mundo empresarial, con esta temática, exponiéndoles qué son las patentes, su importancia, y cómo el conocimiento de este tipo de información y su uso efectivo se ha hecho indispensable en el quehacer creativo del movimiento científico e innovador.

  17. Breve Historia de la Neurocirugía.

    Directory of Open Access Journals (Sweden)

    Germán Peña Quiñones

    2005-06-01

    El primero en desafiar los conceptos hipocráticos fue Galeno de Pérgamo (129-210AD. Cuenta la leyenda que fue hijo de Nicon un arquitecto de Pérgamo (Ionia, hoy Turquía, que fue visitado durante el sueño por Aesclepiades (Esculapio quien le dijo que su hijo debería estudiar medicina y así fue como Galeno entró muy joven al Aesculapion de Pérgamo, un centro muy famoso, dedicado a la actividad religiosa y a la ciencia de curar, donde se concentraban las personas mas importantes en las diversas disciplinas de la medicina y donde Galeno aprendió a curar basándose en información sobre conceptos anatómicos y de fisiología. Posteriormente recorrió centros similares de cultura médica en diversas islas griegas hasta llegar a Alejandría, el sitio más importante para estudios de anatomía y fisiología del mundo antiguo, donde permaneció hasta los 28 años cuando volvió a Pérgamo. Allí se dedicó a tratar a los gladiadores y combinó lo que aprendía de los heridos con experimentos en animales. Posteriormente viajó a Roma donde continuó haciendo observaciones en el Coliseo, donde trabajaba. Durante esta época escribió aproximadamente 500 tratados sobre filosofía, ciencia y medicina, en griego ático; desafortunadamente estos fueron destruidos en su mayor parte durante un incendio en el año 191; de los escritos que quedaron, se puede derivar que Galeno desafió los conceptos de Hipócrates, basándose en sus conocimientos obtenidos por medio de observación, experimentación y disección. Durante esta época gobernaron a Roma los emperadores Antonious Pius y Marcus Aurelius y Galeno, como médico de los gladiadores pudo observar y tratar todo tipo de traumas y heridas. Diferenció la piamadre de la duramadre, describió el sistema ventricular, los signos de hidrocefalia y las glándulas pineal y pituitaria. Describió la vena que hoy lleva su nombre e hizo múltiples cirugías experimentales en monos que le permitieron establecer que los animales no

  18. Breve retrospectiva do desenvolvimento das atividades de auditoria no Brasil

    Directory of Open Access Journals (Sweden)

    Álvaro Ricardino

    2004-08-01

    Full Text Available É difícil determinar a exata data em que foi procedido o primeiro trabalho de Auditoria no Brasil, mas, há exatamente um século, o balanço da São Paulo Tramway Light & Power Co., relativo ao período compreendido entre junho de 1899 e 31 de dezembro de 1902, foi certificado pela empresa canadense de Auditoria Clarkson & Cross - atualmente Ernst & Young. Este artigo procura recuperar a memória desse período, basicamente sob três ângulos: técnicooperacional, acadêmico e legal. Em termos operacionais, a primeira empresa de Auditoria Independente a se estabelecer no Brasil foi a DeloitteToucheTohmatsu, que instalou seu primeiro escritório no Rio de Janeiro, em 1911, e o segundo em Recife, em 1917. Àquela época, o país estava longe de ter qualquer tipo de preocupação com o assunto. Um dos primeiros artigos sobre esse tema foi publicado, em 1928, pela Revista Paulista de Contabilidade, com o título "Contabilidade na Grã-bretanha". O primeiro livro sobre Auditoria foi publicado apenas em 1957 e denominado "Curso de Auditoria". No que se refere ao aspecto legal, os conceitos e técnicas de auditoria foram formalmente introduzidos no ensino universitário por força do Decreto-Lei 7.988, de 22 de dezembro de 1945, o qual regulamentava a educação superior nos cursos de Economia, Contabilidade e Ciências Atuariais. Ainda no âmbito das leis, somente em 1965, pela primeira vez, foi introduzida afigura do Auditor Independente em um diploma legal. Esse foi um marco de enorme importância para a evolução da profissão e das práticas contábeis. Desde então, tanto as práticas de Auditoria quanto seu ensino acadêmico se consolidaram no país, de forma a atender seu principal objetivo: opinar a respeito da adequação das demonstrações contábeis publicadas, no que diz respeito aos seus aspectos mais relevantes.It is hard to determine the exact date of the first auditing work in Brazil but, about one century ago, the balance sheet

  19. Effect of Bifidobacterium breve M-16V supplementation on faecal bifidobacteria in growth restricted very preterm infants - analysis from a randomised trial.

    Science.gov (United States)

    Patole, Sanjay K; Keil, Anthony D; Nathan, Elizabeth; Doherty, Dorota; Esvaran, Meera; Simmer, Karen N; Conway, Patricia

    2016-12-01

    Gut development, function and colonisation are impaired in animal models of prematurity with intrauterine growth restriction (IUGR). The effect of Bifidobacterium breve (B. breve) supplementation on faecal bifidobacteria in small for gestational age (SGA: birth weight breve M-16V supplementation effect on faecal bifidobacteria in preterm (breve counts between SGA versus non-SGA infants [probiotic: 7 (33%) versus 22 (42%), p = 0.603; placebo: 1 (6%) versus 1 (2%), p = 0.429]. B. breve counts did not differ between SGA and non-SGA infants in response to treatment (p = 0.589), after adjusting for baseline count (p breve M-16V supplementation was not significantly different in preterm (<33 weeks) SGA versus non-SGA infants.

  20. Differences in acid tolerance between Bifidobacterium breve BB8 and its acid-resistant derivative B. breve BB8dpH, revealed by RNA-sequencing and physiological analysis.

    Science.gov (United States)

    Yang, Xu; Hang, Xiaomin; Tan, Jing; Yang, Hong

    2015-06-01

    Bifidobacteria are common inhabitants of the human gastrointestinal tract, and their application has increased dramatically in recent years due to their health-promoting effects. The ability of bifidobacteria to tolerate acidic environments is particularly important for their function as probiotics because they encounter such environments in food products and during passage through the gastrointestinal tract. In this study, we generated a derivative, Bifidobacterium breve BB8dpH, which displayed a stable, acid-resistant phenotype. To investigate the possible reasons for the higher acid tolerance of B. breve BB8dpH, as compared with its parental strain B. breve BB8, a combined transcriptome and physiological approach was used to characterize differences between the two strains. An analysis of the transcriptome by RNA-sequencing indicated that the expression of 121 genes was increased by more than 2-fold, while the expression of 146 genes was reduced more than 2-fold, in B. breve BB8dpH. Validation of the RNA-sequencing data using real-time quantitative PCR analysis demonstrated that the RNA-sequencing results were highly reliable. The comparison analysis, based on differentially expressed genes, suggested that the acid tolerance of B. breve BB8dpH was enhanced by regulating the expression of genes involved in carbohydrate transport and metabolism, energy production, synthesis of cell envelope components (peptidoglycan and exopolysaccharide), synthesis and transport of glutamate and glutamine, and histidine synthesis. Furthermore, an analysis of physiological data showed that B. breve BB8dpH displayed higher production of exopolysaccharide and lower H(+)-ATPase activity than B. breve BB8. The results presented here will improve our understanding of acid tolerance in bifidobacteria, and they will lead to the development of new strategies to enhance the acid tolerance of bifidobacterial strains. Copyright © 2015 Elsevier Ltd. All rights reserved.

  1. Old Testament exegesis: Reflections on methodology

    African Journals Online (AJOL)

    p1243322

    1914:v). 2 Becker (2005:3) states as follows: “Die alttestamentliche Texte haben ein Alter von etwa. 2800 bis 2000 Jahren; sie stammen aus einer anderen kulturellen Welt; sie sind in einer fremden, völlig anders strukturierten Sprache geschrieben. Wer solche Texte verstehen will, muβ sie in unsere Sprach- und Denkwelt ...

  2. Extraction of the same novel homoglycan mixture from two different strains of Bifidobacterium animalis and three strains of Bifidobacterium breve.

    Science.gov (United States)

    Alhudhud, M; Sadiq, S; Ngo, H N; Hidalgo-Cantabrana, C; Ruas-Madiedo, P; van Sinderen, D; Humphreys, P N; Laws, A P

    2018-04-26

    Three strains of Bifidobacterium breve (JCM 7017, JCM 7019 and JCM 2258) and two strains of Bifidobacterium animalis subsp. lactis (AD011 and A1dOxR) were grown in broth cultures or on plates, and a standard exopolysaccharide extraction method was used in an attempt to recover exocellular polysaccharides. When the extracted materials were analysed by NMR it was clear that mixtures of polysaccharides were being isolated including exopolysaccharides (EPS) cell wall polysaccharides and intracellular polysaccharides. Treatment of the cell biomass from the B. breve strains, or the B. animalis subsp. lactis AD011 strain, with aqueous sodium hydroxide provided a very similar mixture of polysaccharides but without the EPS. The different polysaccharides were partially fractionated by selective precipitation from an aqueous solution upon the addition of increasing percentages of ethanol. The polysaccharides extracted from B. breve JCM 7017 grown in HBM media supplemented with glucose (or isotopically labelled D-glucose-1- 13 C) were characterised using 1D and 2D-NMR spectroscopy. Addition of one volume of ethanol generated a medium molecular weight glycogen (Mw=1×10 5 Da, yield 200 mg/l). The addition of two volumes of ethanol precipitated an intimate mixture of a low molecular weight β-(1→6)-glucan and a low molecular weight β-(1→6)-galactofuranan which could not be separated (combined yield 46 mg/l). When labelled D-glucose-1- 13 C was used as a carbon supplement, the label was incorporated into >95% of the anomeric carbons of each polysaccharide confirming they were being synthesised in situ. Similar 1 H NMR profiles were obtained for polysaccharides recovered from the cells of B. animalis subsp. lactis AD011and A1dOxR (in combination with an EPS), B. breve JCM 7017, B. breve JCM 7019, B. breve JCM 2258 and from an EPS (-ve) mutant of B. breve 7017 (a non-EPS producer).

  3. Breve Historia de la Academia Nacional de Medicina

    Directory of Open Access Journals (Sweden)

    Efraim Otero Ruiz

    1992-03-01

    sanatorios para tuberculosis y lepra, las primeras campañas de vacunación, de aislmniento de enfermos contagiosos, de prevención y manejo de desastres, de análisis y tratamiento de aguas públicas, etc.; pero además, trabajando en estrecha colaboración con la Facultad de Medicina de la Universidad Nacional -cuya fundación data de la misma época- fijando las normas que orientaran la educación médica en nuestro país. Efectivamente, fue por interés y acciones de varios académicos por los que ‘Setrajeron al país las primeras misiones francesas que dieron nuevas pautas y ritmos a la enseñanza de la medicina que, como todos sabemos, siguió hasta la postguerra del lIo. conflicto mundial la orientación francesa y europea que le dieron sus patrocinadores.

    En 1933-34 el entonces presidente-electo de la nación, Dr. Alfonso López Pumarejo, encargó a la Academia para que realizara el Primer Estudio Nacional de Salud, ‘cuyas recomendaciones irían a ser aplicadas por su Gobierno e incluidas en la importante reforma constitucional de 1936. De allí surgieron recomendaciones como la de crear el Ministerio deSalud (que remplazara a las Juntas Nacional y Departamentales de Higiene, por entonces existentes y se dictaran las primeras normas sobre seguridad social y saneamiento ambiental: por esa magna tarea la Academia fue condecorada con la Cruz de Boyacá en 1936.

    Adaptándose a los tiempos, y especialmente de 1945 en adelante, la Academia se encargó de analizar y estudiar las primeras acciones de dicho Ministerio y del recientemente creado Instituto de los Seguros Sociales, a finales de la década del 40 y comienzos de los 50 ‘s. Obviamente, su poder normativo y asesor fue disminuyendo, pero su actividad científica se fue depurando y aumentando, pudiéndose decir que los más importantes trabajos de investigación elaborados en Columbia en los últimos 50 años han sido presentados a la Academia.

    Por diversas modificaciones de estatutos y

  4. REFLEXÕES SOBRE MODERNIDADE, TURISMO E CAMPO SOCIAL NO ESTADO DE ALAGOAS - BRASIL

    OpenAIRE

    Daniel Arthur Lisboa de Vasconcelos; Edson José Gouveia Bezerra

    2013-01-01

    O presente artigo, de caráter ensaístico, visa a contribuir com reflexões sobre modernidade, turismo e campo social sociais no Estado de Alagoas - Brasil. Utilizando o referencial de autores como Giddens (2001), Jameson (2002) e Harvey (1992), dentre outros, sintetizaremos algumas características das transições socioculturais da modernidade para o atual estágio do modo de produção capitalista, período que muitos têm considerado como a “pós-modernidade”. Posteriormente, empreenderemos breve de...

  5. El acero que nunca se fundió. Una reseña sobre Stalin

    Directory of Open Access Journals (Sweden)

    Iñaki Oneca Agurruza

    2004-02-01

    Full Text Available Era el 5 de marzo de 1953 cuando murió el fetiche, el gurú, el símbolo. Stalin significa “acero”, y precisamente una de las cosas que se reprochó al joven revolucionario, tras la adopción de este apodo, era que el acero era duro pero también frío. Así iniciamos esta breve nota sobre la figura de uno de los personajes clave del siglo XX.

  6. Bifidobacterium breve M-16V as a Probiotic for Preterm Infants: A Strain-Specific Systematic Review.

    Science.gov (United States)

    Athalye-Jape, Gayatri; Rao, Shripada; Simmer, Karen; Patole, Sanjay

    2017-08-01

    Bifidobacterium breve M-16V has been used as a probiotic in preterm infants. Probiotic strain-specific data are essential to guide clinical practice. To assess effects of B breve M-16V in preterm neonates. A systematic review of randomized controlled trials (RCTs) and non-RCTs of B breve M-16V in preterm infants was conducted. Multiple databases, proceedings of Pediatric Academy Society, and other relevant conferences were searched in September 2016 and on January 5, 2017. Five RCTs (n = 482) and 4 non-RCTs (n = 2496) were included. Of the 5 RCTs, 4 carried high/unclear risk of bias in many domains. Meta-analysis (fixed effects model) of RCTs showed no significant benefits on stage ≥2 necrotizing enterocolitis, late-onset sepsis, mortality, and postnatal age at full feeds. Meta-analysis of non-RCTs showed significant benefits on (1) late-onset sepsis-3 studies (n = 2452), odds ratio = 0.56 (95% CI, 0.45-0.71), P breve M-16V. On Grading of Recommendations, Assessment, Development, and Evaluation analysis, the overall quality of evidence was deemed very low. Current evidence is limited regarding the potential of B breve M-16V in preterm neonates. Adequately powered, preferably cluster RCTs are needed to confirm these findings.

  7. Analysis of infant isolates of Bifidobacterium breve by comparative genome hybridization indicates the existence of new subspecies with marked infant specificity.

    Science.gov (United States)

    Boesten, Rolf; Schuren, Frank; Wind, Richèle D; Knol, Jan; de Vos, Willem M

    2011-09-01

    A total of 20 Bifidobacterium strains were isolated from fecal samples of 4 breast- and bottle-fed infants and all were characterized as Bifidobacterium breve based on 16S rRNA gene sequence and metabolic analysis. These isolates were further characterized and compared to the type strains of B. breve and 7 other Bifidobacterium spp. by comparative genome hybridization. For this purpose, we constructed and used a DNA-based microarray containing over 2000 randomly cloned DNA fragments from B. breve type strain LMG13208. This molecular analysis revealed a high degree of genomic variation between the isolated strains and allowed the vast majority to be grouped into 4 clusters. One cluster contained a single isolate that was virtually indistinguishable from the B. breve type strain. The 3 other clusters included 19 B. breve strains that differed considerably from all type strains. Remarkably, each of the 4 clusters included strains that were isolated from a single infant, indicating that a niche adaptation may contribute to variation within the B. breve species. Based on genomic hybridization data, the new B. breve isolates were estimated to contain approximately 60-90% of the genes of the B. breve type strain, attesting to the existence of various subspecies within the species B. breve. Further bioinformatic analysis identified several hundred diagnostic clones specific to the genomic clustering of the B. breve isolates. Molecular analysis of representatives of these revealed that annotated genes from the conserved B. breve core encoded mainly housekeeping functions, while the strain-specific genes were predicted to code for functions related to life style, such as carbohydrate metabolism and transport. This is compatible with genetic adaptation of the strains to their niche, a combination of infants and diet. Copyright © 2011 Institut Pasteur. Published by Elsevier Masson SAS. All rights reserved.

  8. La medición de síntomas psicológicos y psicosomáticos: el Listado de Síntomas Breve (LSB-50

    Directory of Open Access Journals (Sweden)

    Manuel R. Abuín

    2014-01-01

    Full Text Available El Listado de Síntomas Breve (LSB-50 es un instrumento de evaluación psicopatológica utilizado para el despistaje de síntomas psicológicos y psicosomáticos. El LSB-50 contiene nueve escalas y subescalas clínicas, a las que se ha añadido un índice de riesgo psicopatológico, tres índices globales y dos escalas de validez. El objetivo de este estudio fue estudiar la fiabilidad y validez del instrumento. Se ha empleado una muestra de 1.242 sujetos, 790 pertenecientes a la población general y 452 a muestras clínicas. Los coeficientes de consistencia interna alfa de las escalas oscilan entre .79 y .90, mientras que los estudios de validez tanto factorial como criterial (correlación con variables de dependencia y vínculo interpersonal y análisis discriminantes aportan evidencias de validez de la prueba. Se plantea la discusión sobre la utilidad de los cuestionarios de síntomas para el diagnóstico psicopatológico y posibles implicaciones en las conceptualizaciones de los síndromes clínicos psicopatológicos.

  9. Intestinal Microbiota in Pediatric Surgical Cases Administered Bifidobacterium Breve: A Randomized Controlled Trial.

    Science.gov (United States)

    Okazaki, Tadaharu; Asahara, Takashi; Yamataka, Atsuyuki; Ogasawara, Yuki; Lane, Geoffrey J; Nomoto, Koji; Nagata, Satoru; Yamashiro, Yuichiro

    2016-07-01

    The efficacy of perioperative probiotic administration has been reported in adults. We examined the effects of orally administered Bifidobacterium breve strain Yakult (BBG-01) on outcomes in pediatric surgical cases by assessing intestinal and blood microbiota. BBG-01 was well tolerated without adverse effects, and postoperative infectious complications were significantly decreased. Fecal analysis showed increased Bifidobacterium and decreased Enterobacteriaceae, Clostridium difficile, and Pseudomonas. Concentrations of fecal acetic acid were significantly increased, maintaining fecal pH at <7.0. The incidence of detecting bacteria in blood was significantly reduced. BBG-01 improved the intestinal environment, and may be implicated in suppressing bacterial translocation.

  10. Propuesta de un cuestionario breve para la evaluación de la competitividad en el ámbito deportivo: Competitividad-10

    Directory of Open Access Journals (Sweden)

    Eduardo Remor

    2007-01-01

    Full Text Available Se ha desarrollado y analizado psicométricamente un cuestionario breve para la evaluación de la competitividad en el deporte. El desarrollo de los ítems para el instrumento parte de una revisión bibliográfica sobre las teorías de logro relacionadas con el deporte (validez de contenido. Seguidamente, se realizó un experimento donde se expuso a los participantes a una situación de competición y se utilizó dicha situación como criterio externo para la valoración del grado de competitividad y éxito en la competición (validez empírica. El experimento, y estudio psicométrico, incluyó a 153 deportistas estudiantes universitarios (43,8% mujeres y 56,2% hombres con edades entre los 19 y 32 años (media 22,9 años, DT=2,5. Los resultados del análisis factorial ofrecieron apoyo a la validez de constructo, identificando dos dimensiones independientes: Motivación de éxito (a = .66 y Motivación para evitar el fracaso (a = .66, siendo aceptable la fiabilidad de ambas. También se ha identificado la validez de criterio, concurrente, discriminante y predictiva (sensibilidad y especificidad del cuestionario teniendo en cuenta el resultado en la competición y la autovaloración subjetiva del grado de competitividad. La sensibilidad y especificidad del cuestionario fueron 72,6% y 50,7%, respectivamente. En resumen, los indicadores psicométricos del cuestionario confirman la validez de constructo, y sostienen la fiabilidad y validez empírica del cuestionario. Se han observado diferencias de género y un efecto de la edad sobre la motivación de éxito.

  11. Bifidobacterium breve as a delivery vector of IL-24 gene therapy for head and neck squamous cell carcinoma in vivo.

    Science.gov (United States)

    Wang, L; Vuletic, I; Deng, D; Crielaard, W; Xie, Z; Zhou, K; Zhang, J; Sun, H; Ren, Q; Guo, C

    2017-11-01

    Beneficial bacteria are becoming ever more popular gene delivery method for hypoxia-tumor targeting in vivo. In this study we investigated the therapeutic effect of new recombinant Bifidobacterium breve strain expressing interleukin (IL)-24 gene (B. breve-IL24) on head and neck tumor xenograft in mice. Briefly, B. breve transformants were obtained through electro-transformation. Bacteria-tumor-targeting ability were analyzed in vivo over different time points (1, 3 and 7 days post-bacteria injection). Furthermore, the therapeutic effect of bacteria on tumor cells in vivo were analyzed as follows: 30 Balb/c nude mice bearing subcutaneous tumor were randomly divided in three groups (Drug group, green fluorescent protein (GFP) group and Saline group). The therapy lasted for 2 weeks and included B. breve-IL24 administration via tail vein for Drug group, B. breve-GFP for GFP group and phosphate buffered saline for Saline group. The tumor growth was monitored using standard caliper technique, while the apoptosis induction in vivo was analyzed by Real-time Positron Emission Tomography/Computed Tomography (PET/CT) imaging ([18F]-ML-10 tracer). At the end of the experiment, tumor tissues were collected and analyzed by western blotting. Briefly, our results suggested that our new recombinant bacterium has the capability of targeting tumor tissue in vivo. As for the therapeutic effect, our new strain has revealed to be a promising therapeutic approach against tumor growth in vivo. Briefly, higher tumor growth inhibition and higher tumor cell apoptosis induction were observed in Drug group compared with the GFP and Saline groups. To conclude, a new recombinant strain B. breve-IL24 offers a novel, safe and clinically acceptable therapeutic approach for tumor therapy in vivo.

  12. Post-sensitization administration of non-digestible oligosaccharides and Bifidobacterium breve M-16V reduces allergic symptoms in mice.

    Science.gov (United States)

    van Esch, Betty C A M; Abbring, Suzanne; Diks, Mara A P; Dingjan, Gemma M; Harthoorn, Lucien F; Vos, A Paul; Garssen, Johan

    2016-06-01

    To support dietary management of severe cow's milk allergic infants, a synbiotic mixture of non-digestible oligosaccharides and Bifidobacterium breve M-16V ( B. breve ) was designed from source materials that are completely cow's milk-free. It was investigated whether this specific synbiotic concept can reduce an established food allergic response in a research model for hen's egg allergy. Mice were orally sensitized once a week for 5 weeks to ovalbumin (OVA) using cholera toxin (CT) as an adjuvant. Non-sensitized mice received CT in PBS only. Sensitized mice were fed a control diet or a diet enriched with short-chain- (scFOS) and long-chain fructo-oligosaccharides (lcFOS), B. breve or scFOSlcFOS +  B. breve for 3 weeks starting after the last sensitization. Non-sensitized mice received the control diet. Anaphylactic shock symptoms, acute allergic skin responses and serum specific IgE, mMCP-1 and galectin-9 were measured upon OVA challenge. Activated Th2-, Th1-cells and regulatory T-cells were quantified in spleen and mesenteric lymph nodes (MLN) and cytokine profiles were analyzed. Short chain fatty acids (SCFA) were measured in ceacal samples. The acute allergic skin response was reduced in mice fed the scFOSlcFOS +  B. breve diet compared to mice fed any of the other diets. A reduction in mast cell degranulation (mMCP-1) and anaphylactic shock symptoms was also observed in these mice. Unstimulated splenocyte cultures produced increased levels of IL10 and IFNg in mice fed the scFOSlcFOS +  B. breve diet. Correspondingly, increased percentages of activated Th1 cells were observed in the spleen. Allergen-specific re-stimulation of splenocytes showed a decrease in IL5 production. In summary; post-sensitization administration of scFOSlcFOS +  B. breve was effective in reducing allergic symptoms after allergen challenge. These effects coincided with changes in regulatory and effector T-cell subsets and increases in the SCFA propionic acid. These

  13. Biotransformation of rutin to isoquercitrin using recombinant α-L-rhamnosidase from Bifidobacterium breve.

    Science.gov (United States)

    Zhang, Ru; Zhang, Bian-Ling; Xie, Tao; Li, Gu-Cai; Tuo, Yi; Xiang, Yu-Ting

    2015-06-01

    To biotransform rutin into isoquercitrin. A α-L-rhamnosidase from Bifidobacterium breve was produced by using Escherichia coli BL21 for biotransformation of rutin to isoquercitrin. The enzyme was purified by Ni(2+)-NTA chromatography to yield a soluble protein with a specific activity of 56 U protein mg(-1). The maximum enzyme activities were at pH 6.5, 55 °C, 20 mM rutin, and 1.2 U enzyme ml(-1). Under optimal conditions, the half-life of the enzyme was 96 h. The K m and V max values were 2.2 mM, 56.4 μmol mg(-1) min(-1) and 2.1 mM, 57.5 μmol mg(-1) min(-1) using pNP-Rha and rutin as substrates, respectively. The kinetic behavior indicated that the recombinant α-L-rhamnosidase has good catalytic performance for producing isoquercitrin. 20 mM rutin was biotransformed into 18.25 and 19.87 mM isoquercitrin after 60 and 240 min. The specific biotransformation of rutin to isoquercitrin using recombinant α-L-rhamnosidase from B. breve is a feasible method for use in industrial processes.

  14. Coculture of Bifidobacterium longum and Bifidobacterium breve alters their protein expression profiles and enzymatic activities.

    Science.gov (United States)

    Ruiz, Lorena; Sánchez, Borja; de Los Reyes-Gavilán, Clara G; Gueimonde, Miguel; Margolles, Abelardo

    2009-07-31

    Some strains of the genus Bifidobacterium are probiotic bacteria commonly added to functional dairy products. The influence of coculturing Bifidobacterium longum NCIMB8809 and Bifidobacterium breve NCIMB8807 on their physiology was studied. 2DE separation of protein extracts, coupled to MS protein analysis allowed the identification of 16 proteins whose expression drastically changed when cells were grown in compartmentalized coculture, compared to monoculture. These included ribosomal proteins and proteins involved in carbohydrate metabolism, gene regulation, cell envelope biogenesis and transport processes. Significant changes in some glycoside-hydrolysing activities (beta-d-xylopyranosidase, alpha-l-arabinofuranosidase and beta-d-glucopyranosidase) were also detected. Furthermore, qRT-PCR experiments using as targets the B. breve genes clgR (transcriptional regulator) clpP1, clpP2 and clpC (chaperone- and protease-encoding genes positively regulated by clgR) supported the proteomic results, the four genes displaying a higher expression level in coculture. This study provides new insights to understand the communication among Bifidobacterium species.

  15. [Mechanism of the inhibitory action of allelochemical dibutyl phthalate on algae Gymnodinium breve].

    Science.gov (United States)

    Bie, Cong-Cong; Li, Feng-Min; Wang, Yi-Fei; Wang, Hao-Yun; Zhao, Ya-Han; Zhao, Wei; Wang, Zhen-Yu

    2012-01-01

    The aim of this study was to investigate the mechanism of inhibitory action of dibutyl phthalate (DBP) on red tide algae Gymnodinium breve. The effects of DBP on malonaldehyde, subcellular structure and superoxide dismutase (SOD) isoforms were investigated. The results showed that MDA accumulated in the algae cell under DBP exposure, and for the 3 mg x L(-1) DBP treated algae culture a peak value of 0.34 micromol x (10(9) cells) (-1) occurred at 72 h, which was about 2. 3 times than that of the control. TEM pictures showed the disruption of DBP on the subcellular structure of G. breve. A morphological phenomenon appeared that the algae cell was commonly found small tubules or apical parts around the cell membrane, and almost all normal cell organelles were indistinguishable finally. The activity of CuZn-SOD (main cytoplast located isoform with little in cloroplast) under DBP exposure was higher than that of the control, and no significant difference was observed on Fe-SOD (chloroplast located isoform) activity, but for the Mn-SOD (mitochondrial isoform), the activity was significantly inhibited. These results indicated that DBP might inhibit the algae growth from the plasma membrane and the mitochondria, resulting in oxidative damage in algae cell and a final death. This paper will give a theoretical support to the practical usage of the allelochemical on red tide algae.

  16. [Effects of allelochemical dibutyl phthalate on Gymnodinium breve reactive oxygen species].

    Science.gov (United States)

    Bie, Cong-Cong; Li, Feng-Min; Li, Yuan-Yuan; Wang, Zhen-Yu

    2012-02-01

    The purpose of this study was to investigate the mechanism of inhibitory action of dibutyl phthalate (DBP) on red tide algae Gymnodinium breve. Reactive oxygen species (ROS) level, contents of *OH and H2O2, and O2*(-) production rate were investigated, and also for the effects of electron transfer inhibitors on the ROS induction of DBP. The results showed that DBP triggered the synthesis of reactive oxygen species ROS, and with the increase of concentration of DBP, *OH and H2O2 contents in cells accumulated, as for the 3 mg x L(-1) DBP treated algae cultures, OH showed a peak of 33 U x mL(-1) at 48 h, which was about 2. 4 times higher than that in the controlled, and H2O2 contents was about 250 nmol x (10(7) cells)(-1) at 72 h, which was about 5 times higher and also was the highest during the whole culture. Rotenone (an inhibitor of complex I in the mitochondria electron transport chain) decreased the DBP induced ROS production, and dicumarol (an inhibitor of the redox enzyme system in the plasma membrane) stimulated the DBP induced ROS production. Taken all together, the results demonstrated DBP induced over production of reactive oxygen species in G. breve, which is the main inhibitory mechanism, and mitochondria and plasma membrane seem to be the main target site of DBP. These conclusions were of scientific meaning on uncovering the inhibitory mechanism of allelochemical on algae.

  17. Breve historia

    Directory of Open Access Journals (Sweden)

    Graciela Herrera Labra

    2002-01-01

    Full Text Available Los antecedentes histórico-educativos de los pueblos indios que aquí se mencionan son el resultado de un largo proceso histórico que adquiere gran complejidad en el periodo colonial y que se cristaliza en la actual nación mexicana. La idea de trabajar estos antecedentes, es con el objeto de señalar que en el marco de la educación indígena siempre ha estado presente en su historia la mediación de la conquista. El recorte histórico que se presenta en este trabajo parte de la creación de la licenciatura en educación indígena que se imparte en la UPN. Partir de este momento histórico es con la siguiente finalidad: ● Conocer las diferentes acciones educativas que abrieron brecha a la actual profesionalización indígena.

  18. Complete genome sequence of the probiotic bacterium Bifidobacterium breve KCTC 12201BP isolated from a healthy infant.

    Science.gov (United States)

    Kwak, Min-Jung; Yoon, Jae-Kyung; Kwon, Soon-Kyeong; Chung, Myung-Jun; Seo, Jae-Gu; Kim, Jihyun F

    2015-11-20

    We present the completely sequenced genome of Bifidobacterium breve CBT BR3, which was isolated from the feces of a healthy infant. The 2.43-Mb genome contains several kinds of genetic factors associated with health promotion of the human host such as oligosaccharide-degrading genes and vitamin-biosynthetic genes. Copyright © 2015 Elsevier B.V. All rights reserved.

  19. A Conserved Two-Component Signal Transduction System Controls the Response to Phosphate Starvation in Bifidobacterium breve UCC2003.

    NARCIS (Netherlands)

    Alvarez-Martin, P.; Fernandez, M.; O'Connell-Motherway, M.; O'Connell, K.J.; Sauvageot, N.; Fitzgerald, G.F.; Macsharry, J.; Zomer, A.L.; Sinderen, D. van

    2012-01-01

    This work reports on the identification and molecular characterization of the two-component regulatory system (2CRS) PhoRP, which controls the response to inorganic phosphate (P(i)) starvation in Bifidobacterium breve UCC2003. The response regulator PhoP was shown to bind to the promoter region of

  20. A conserved two-component signal transduction system controls the response to phosphate starvation in Bifidobacterium breve UCC2003.

    Science.gov (United States)

    Alvarez-Martin, Pablo; Fernández, Matilde; O'Connell-Motherway, Mary; O'Connell, Kerry Joan; Sauvageot, Nicolas; Fitzgerald, Gerald F; MacSharry, John; Zomer, Aldert; van Sinderen, Douwe

    2012-08-01

    This work reports on the identification and molecular characterization of the two-component regulatory system (2CRS) PhoRP, which controls the response to inorganic phosphate (P(i)) starvation in Bifidobacterium breve UCC2003. The response regulator PhoP was shown to bind to the promoter region of pstSCAB, specifying a predicted P(i) transporter system, as well as that of phoU, which encodes a putative P(i)-responsive regulatory protein. This interaction is assumed to cause transcriptional modulation under conditions of P(i) limitation. Our data suggest that the phoRP genes are subject to positive autoregulation and, together with pstSCAB and presumably phoU, represent the complete regulon controlled by the phoRP-encoded 2CRS in B. breve UCC2003. Determination of the minimal PhoP binding region combined with bioinformatic analysis revealed the probable recognition sequence of PhoP, designated here as the PHO box, which together with phoRP is conserved among many high-GC-content Gram-positive bacteria. The importance of the phoRP 2CRS in the response of B. breve to P(i) starvation conditions was confirmed by analysis of a B. breve phoP insertion mutant which exhibited decreased growth under phosphate-limiting conditions compared to its parent strain UCC2003.

  1. Comparative genome and methylome analysis reveals restriction/modification system diversity in the gut commensal Bifidobacterium breve

    Science.gov (United States)

    Bottacini, Francesca; Morrissey, Ruth; Roberts, Richard John; James, Kieran; van Breen, Justin; Egan, Muireann; Lambert, Jolanda; van Limpt, Kees; Knol, Jan; Motherway, Mary O’Connell; van Sinderen, Douwe

    2018-01-01

    Abstract Bifidobacterium breve represents one of the most abundant bifidobacterial species in the gastro-intestinal tract of breast-fed infants, where their presence is believed to exert beneficial effects. In the present study whole genome sequencing, employing the PacBio Single Molecule, Real-Time (SMRT) sequencing platform, combined with comparative genome analysis allowed the most extensive genetic investigation of this taxon. Our findings demonstrate that genes encoding Restriction/Modification (R/M) systems constitute a substantial part of the B. breve variable gene content (or variome). Using the methylome data generated by SMRT sequencing, combined with targeted Illumina bisulfite sequencing (BS-seq) and comparative genome analysis, we were able to detect methylation recognition motifs and assign these to identified B. breve R/M systems, where in several cases such assignments were confirmed by restriction analysis. Furthermore, we show that R/M systems typically impose a very significant barrier to genetic accessibility of B. breve strains, and that cloning of a methyltransferase-encoding gene may overcome such a barrier, thus allowing future functional investigations of members of this species. PMID:29294107

  2. BIO-OPTICAL CHARACTERIZATION OF THE DINOFLAGELLATE GYMNODINIUM BREVE AND THE DIATOM THALASSIOSIRA WEISSFLOGII IN OUTDOOR TANKS

    Science.gov (United States)

    The bio-optical signatures of harmful algal blooms can be used to define ocean color satellite algorithms. We characterized the bio-optical properties of nutrient-replete cultures of the red tide dinoflagellate Gymnodinium breve and the diatom Thalassiosira weissflogii. We cultur...

  3. Algumas considerações sobre a sociologia de Alfred Schutz

    Directory of Open Access Journals (Sweden)

    Márcio Nicory Costa Souza

    2012-07-01

    Full Text Available http://dx.doi.org/10.5007/1806-5023.2012v9n1p1 O presente artigo apresenta algumas considerações sobre a perspectiva sociológica de Alfred Schutz. Para tal, tecemos breves considerações sobre a fenomenologia, em especial a partir das contribuições de Husserl. Trata-se, portanto, de um esforço para o entendimento de sua importância para as Ciências Sociais. Primeiramente, apresentamos as influências constitutivas do pensamento de Schutz, bem como suas principais ferramentas analíticas. Em seguida, pensando no “fazer ciência”, procedemos para reflexões analíticas da proposta de uma Sociologia Interpretativa.

  4. Bifidobacterium breve BBG-001 in very preterm infants: a randomised controlled phase 3 trial.

    Science.gov (United States)

    Costeloe, Kate; Hardy, Pollyanna; Juszczak, Edmund; Wilks, Mark; Millar, Michael R

    2016-02-13

    Probiotics may reduce necrotising enterocolitis and late-onset sepsis after preterm birth. However, there has been concern about the rigour and generalisability of some trials and there is no agreement about whether or not they should be used routinely. We aimed to test the effectiveness of the probiotic Bifidobacterium breve BBG-001 to reduce necrotising enterocolitis, late-onset sepsis, and death in preterm infants. In this multicentre, randomised controlled phase 3 study (the PiPS trial), we recruited infants born between 23 and 30 weeks' gestational age within 48 h of birth from 24 hospitals in southeast England. Infants were randomly assigned (1:1) to probiotic or placebo via a minimisation algorithm randomisation programme. The probiotic intervention was B breve BBG-001 suspended in dilute elemental infant formula given enterally in a daily dose of 8·2 to 9·2 log10 CFU; the placebo was dilute infant formula alone. Clinicians and families were masked to allocation. The primary outcomes were necrotising enterocolitis (Bell stage 2 or 3), blood culture positive sepsis more than 72 h after birth; and death before discharge from hospital. All primary analyses were by intention to treat. This trial is registered with ISRCTN, number 05511098 and EudraCT, number 2006-003445-17. Between July 1, 2010, and July 31, 2013, 1315 infants were recruited; of whom 654 were allocated to probiotic and 661 to placebo. Five infants had consent withdrawn after randomisation, thus 650 were analysed in the probiotic group and 660 in the placebo group. Rates of the primary outcomes did not differ significantly between the probiotic and placebo groups. 61 infants (9%) in the probiotic group had necrotising enterocolitis compared with 66 (10%) in the placebo group (adjusted risk ratio 0·93 (95% CI 0·68-1·27); 73 (11%) infants in the probiotics group had sepsis compared with 77 (12%) in the placebo group (0·97 (0·73-1·29); and 54 (8%) deaths occurred before discharge home in the

  5. Bifidobacterium breve C50 secretes lipoprotein with CHAP domain recognized in aggregated form by TLR2.

    Science.gov (United States)

    Scuotto, Angelo; Djorie, Serge; Colavizza, Michel; Romond, Pierre-Charles; Romond, Marie-Bénédicte

    2014-12-01

    Extracellular components secreted by Bifidobacterium breve C50 can induce maturation, high IL-10 production and prolonged survival of dendritic cells via a TLR2 pathway. In this study, the components were isolated from the supernatant by gel filtration chromatography. Antibodies raised against the major compounds with molecular weight above 600 kDa (Bb C50BC) also recognized compounds of lower molecular weight (200–600 kDa). TLR2 and TLR6 bound to the components already recognized by the antibodies. Trypsin digestion of Bb C50BC released three major peptides whose sequences displayed close similarities to a putative secreted protein with a CHAP amidase domain from B. breve. The 1300-bp genomic region corresponding to the hypothetical protein was amplified by PCR. The deduced polypeptide started with an N-terminal signal sequence of 45 amino acids, containing the lipobox motif (LAAC) with the cysteine in position 25, and 2 positively charged residues within the first 14 residues of the signal sequence. Lipid detection in Bb C50BC by GC/MS further supported the implication of a lipoprotein. Sugars were also detected in Bb C50BC. Close similarity with the glucan-binding protein B from Bifidobacterium animalis of two released peptides from Bb C50BC protein suggested that glucose moieties, possibly in glucan form, could be bound to the lipoprotein. Finally, heating at 100 °C for 5 min led to the breakdown of Bb C50BC in compounds of molecular weight below 67 kDa, which suggested that Bb C50BC was an aggregate. One might assume that a basic unit was formed by the lipoprotein bound putatively to glucan. Besides the other sugars and hexosamines recognized by galectin 1 were localized at the surface of the Bb C50BC aggregate. In conclusion, the extracellular components secreted by B. breve C50 were constituted of a lipoprotein putatively associated with glucose moieties and acting in an aggregating form as an agonist of TLR2/TLR6.

  6. Improving the storage stability of Bifidobacterium breve in low pH fruit juice.

    Science.gov (United States)

    Saarela, M; Alakomi, H L; Mättö, J; Ahonen, A M; Puhakka, A; Tynkkynen, S

    2011-09-01

    Bifidobacterial food applications are limited since bifidobacteria are sensitive to e.g. acidic conditions prevalent in many food matrices. The aim of the present study was to investigate whether a low pH selection step alone or combined to UV mutagenesis could improve the viability of an acid sensitive Bifidobacterium strain, B. breve 99, in low pH food matrices. Furthermore, the potential of carriers and an oat fibre preparation to further improve the stability was studied. The best performing low pH tolerant variants in the present study were generated by UV-mutagenesis with 70-700μJ/cm(2) followed by incubation in growth medium at pH 4.5. The most promising variants regarding the low pH tolerance showed, in repeated tests with cells grown without pH control, about one Log-value better survival in pH 3.8 fruit juice after one week storage at 4°C compared to wild-type B. breve 99. Cells grown with pH control, PDX formulated and then frozen showed poorer viability in low pH fruit juice than cells grown with no pH control. For frozen concentrates pH 3.8 was too stressful and no or small differences between the variants and the wild-type strain were seen. The differences detected at pH 3.8 with the cells grown without pH control were also seen with the frozen concentrates at pH 4.5. Some improvement in the stability could be achieved by using a combination of trehalose, vitamin C and PDX as a freezing carrier material, whereas a significant improvement in the stability was seen when oat fibre was added into the fruit juice together with the frozen cells. Due to the initial very poor fruit juice tolerance of B. breve 99 the obtained improvement in the stability was not enough for commercial applications. However, the same methods could be applied to initially better performing strains to further improve their stability in the fruit juice. Copyright © 2010 Elsevier B.V. All rights reserved.

  7. Bifidobacterium breve MCC-117 Induces Tolerance in Porcine Intestinal Epithelial Cells: Study of the Mechanisms Involved in the Immunoregulatory Effect

    Science.gov (United States)

    MURATA, Kozue; TOMOSADA, Yohsuke; VILLENA, Julio; CHIBA, Eriko; SHIMAZU, Tomoyuki; ASO, Hisashi; IWABUCHI, Noriyuki; XIAO, Jin-zhong; SAITO, Tadao; KITAZAWA, Haruki

    2014-01-01

    Bifidobacterium breve MCC-117 is able to significantly reduce the expression of inflammatory cytokines in porcine intestinal epithelial (PIE) cells and to improve IL-10 levels in CD4+CD25high Foxp3+ lymphocytes in response to heat-stable enterotoxigenic Escherichia coli (ETEC) pathogen-associated molecular patterns (PAMPs), while the immunoregulatory effect of B. adolescentis ATCC15705 was significantly lower than that observed for the MCC-117 strain. Considering the different capacities of the two bifidobacterium strains to activate toll-like receptor (TLR)-2 and their differential immunoregulatory activities in PIE and immune cells, we hypothesized that comparative studies with both strains could provide important information regarding the molecular mechanism(s) involved in the anti-inflammatory activity of bifidobacteria. In this work, we demonstrated that the anti-inflammatory effect of B. breve MCC-117 was achieved by a complex interaction of multiple negative regulators of TLRs as well as inhibition of multiple signaling pathways. We showed that B. breve MCC-117 reduced heat-stable ETEC PAMP-induced NF-κB, p38 MAPK and PI3 K activation and expression of pro-inflammatory cytokines in PIE cells. In addition, we demonstrated that B. breve MCC-117 may activate TLR2 synergistically and cooperatively with one or more other pattern recognition receptors (PRRs), and that interactions may result in a coordinated sum of signals that induce the upregulation of A20, Bcl-3, Tollip and SIGIRR. Upregulation of these negative regulators could have an important physiological impact on maintaining or reestablishing homeostatic TLR signals in PIE cells. Therefore, in the present study, we gained insight into the molecular mechanisms involved in the immunoregulatory effect of B. breve MCC-117. PMID:24936377

  8. Bifidobacterium breve with α-linolenic acid alters the composition, distribution and transcription factor activity associated with metabolism and absorption of fat.

    Science.gov (United States)

    Patterson, Elaine; Wall, Rebecca; Lisai, Sara; Ross, R Paul; Dinan, Timothy G; Cryan, John F; Fitzgerald, Gerald F; Banni, Sebastiano; Quigley, Eamonn M; Shanahan, Fergus; Stanton, Catherine

    2017-03-07

    This study focused on the mechanisms that fatty acid conjugating strains - Bifidobacterium breve NCIMB 702258 and Bifidobacterium breve DPC 6330 - influence lipid metabolism when ingested with α-linolenic acid (ALA) enriched diet. Four groups of BALB/c mice received ALA enriched diet (3% (w/w)) either alone or in combination with B. breve NCIMB 702258 or B. breve DPC 6330 (10 9 CFU/day) or unsupplemented control diet for six weeks. The overall n-3 PUFA score was increased in all groups receiving the ALA enriched diet. Hepatic peroxisomal beta oxidation increased following supplementation of the ALA enriched diet with B. breve (P breve NCIMB 702258 was found on the endocannabinoid system (ECS). Liver triglycerides (TAG) were reduced following ALA supplementation, compared with unsupplemented controls (P breve further reduced liver TAG (P < 0.01), compared with the ALA enriched control. These data indicate that the interactions of the gut microbiota with fatty acid metabolism directly affect host health by modulating n-3 PUFA score and the ECS.

  9. Bifidobacterium breve with α-linolenic acid alters the composition, distribution and transcription factor activity associated with metabolism and absorption of fat

    Science.gov (United States)

    Patterson, Elaine; Wall, Rebecca; Lisai, Sara; Ross, R. Paul; Dinan, Timothy G.; Cryan, John F.; Fitzgerald, Gerald F.; Banni, Sebastiano; Quigley, Eamonn M.; Shanahan, Fergus; Stanton, Catherine

    2017-01-01

    This study focused on the mechanisms that fatty acid conjugating strains - Bifidobacterium breve NCIMB 702258 and Bifidobacterium breve DPC 6330 - influence lipid metabolism when ingested with α-linolenic acid (ALA) enriched diet. Four groups of BALB/c mice received ALA enriched diet (3% (w/w)) either alone or in combination with B. breve NCIMB 702258 or B. breve DPC 6330 (109 CFU/day) or unsupplemented control diet for six weeks. The overall n-3 PUFA score was increased in all groups receiving the ALA enriched diet. Hepatic peroxisomal beta oxidation increased following supplementation of the ALA enriched diet with B. breve (P breve NCIMB 702258 was found on the endocannabinoid system (ECS). Liver triglycerides (TAG) were reduced following ALA supplementation, compared with unsupplemented controls (P breve further reduced liver TAG (P < 0.01), compared with the ALA enriched control. These data indicate that the interactions of the gut microbiota with fatty acid metabolism directly affect host health by modulating n-3 PUFA score and the ECS. PMID:28265110

  10. Effect of Bifidobacterium breve M-16V supplementation on fecal bifidobacteria in preterm neonates--a randomised double blind placebo controlled trial.

    Science.gov (United States)

    Patole, Sanjay; Keil, Anthony D; Chang, Annie; Nathan, Elizabeth; Doherty, Dorota; Simmer, Karen; Esvaran, Meera; Conway, Patricia

    2014-01-01

    Probiotic supplementation significantly reduces the risk of necrotising enterocolitis (NEC) and all cause mortality in preterm neonates. Independent quality assessment is important before introducing routine probiotic supplementation in this cohort. To assess product quality, and confirm that Bifidobacterium breve (B. breve) M-16V supplementation will increase fecal B. breve counts without adverse effects. Strain identity (16S rRNA gene sequencing), viability over 2 year shelf-life were confirmed, and microbial contamination of the product was ruled out. In a controlled trial preterm neonates (Gestation breve M-16V (3×109 cfu/day) or placebo (dextrin) supplementation until the corrected age 37 weeks. Stool samples were collected before (S1) and after 3 weeks of supplementation (S2) for studying fecal B. breve levels using quantitative PCR (Primary outcome). Secondary outcomes included total fecal bifidobacteria and NEC≥Stage II. Categorical and continuous outcomes were analysed using Chi-square and Mann-Whitney tests, and McNemar and Wilcoxon signed-rank tests for paired comparisons. A total of 159 neonates (Probiotic: 79, Placebo: 80) were enrolled. Maternal and neonatal demographic characteristics were comparable between the groups. The proportion of neonates with detectable B. breve increased significantly post intervention: Placebo: [S1:2/66 (3%), S2: 25/66 (38%), pbreve counts in both groups were below detection (breve M-16V is a suitable probiotic strain for routine use in preterm neonates. Australia New Zealand Clinical Trial Registry ACTRN 12609000374268.

  11. Digital signage sobre IP

    OpenAIRE

    Casademont Filella, Albert

    2012-01-01

    Projecte que versa sobre el disseny i la implementació d'un sistema de Digital Signage (Cartelleria Digital) sobre IP, creant un gestor que permeti controlar de forma remota tot un conjunt de petits dispositius connectats a pantalles que emeten continguts multimèdia com vídeos, imatges, feeds rss...

  12. Epithelial cell proliferation arrest induced by lactate and acetate from Lactobacillus casei and Bifidobacterium breve.

    Directory of Open Access Journals (Sweden)

    Takahiro Matsuki

    Full Text Available In an attempt to identify and characterize how symbiotic bacteria of the gut microbiota affect the molecular and cellular mechanisms of epithelial homeostasis, intestinal epithelial cells were co-cultured with either Lactobacillus or Bifidobacterium as bona fide symbionts to examine potential gene modulations. In addition to genes involved in the innate immune response, genes encoding check-point molecules controlling the cell cycle were among the most modulated in the course of these interactions. In the m-ICcl2 murine cell line, genes encoding cyclin E1 and cyclin D1 were strongly down regulated by L. casei and B. breve respectively. Cell proliferation arrest was accordingly confirmed. Short chain fatty acids (SCFA were the effectors of this modulation, alone or in conjunction with the acidic pH they generated. These results demonstrate that the production of SCFAs, a characteristic of these symbiotic microorganisms, is potentially an essential regulatory effector of epithelial proliferation in the gut.

  13. Discovering Novel Bile Protection Systems in Bifidobacterium breve UCC2003 through Functional Genomics

    Science.gov (United States)

    Ruiz, Lorena; Zomer, Aldert; O'Connell-Motherway, Mary; van Sinderen, Douwe

    2012-01-01

    Tolerance of gut commensals to bile salt exposure is an important feature for their survival in and colonization of the intestinal environment. A transcriptomic approach was employed to study the response of Bifidobacterium breve UCC2003 to bile, allowing the identification of a number of bile-induced genes with a range of predicted functions. The potential roles of a selection of these bile-inducible genes in bile protection were analyzed following heterologous expression in Lactococcus lactis. Genes encoding three transport systems belonging to the major facilitator superfamily (MFS), Bbr_0838, Bbr_0832, and Bbr_1756, and three ABC-type transporters, Bbr_0406-0407, Bbr_1804-1805, and Bbr_1826-1827, were thus investigated and shown to provide enhanced resistance and survival to bile exposure. This work significantly improves our understanding as to how bifidobacteria respond to and survive bile exposure. PMID:22156415

  14. Effects of Gymnodinium breve toxin on the smooth muscle preparation of guinea-pig ileum

    Science.gov (United States)

    Grunfeld, Y.; Spiegelstein, M.Y.

    1974-01-01

    1 The effects of Gymnodinium breve neurotoxin (GT) on smooth muscles were studied using the guinea-pig isolated ileum. 2 The toxin caused strong spasmogenic effects at 1-4 μg/ml, characterized by prolonged tonic contraction with superimposed pronounced pendular movements. Tachyphylaxis was observed upon administration of successive doses. 3 Atropine blocked the contractile response elicited by GT, whereas mepyramine and hexamethonium failed to do so. These findings tentatively suggested a cholinergic involvement at a post-ganglionic site of action. 4 In the presence of tetrodotoxin the effects of GT were abolished, excluding direct action of the toxin on the smooth muscle. 5 It is concluded that GT exerts its spasmogenic effects through stimulation of the post-ganglionic cholinergic nerve fibres. PMID:4155337

  15. Um caso de transtorno de personalidade borderline atendido em psicoterapia dinâmica breve

    Directory of Open Access Journals (Sweden)

    Cunha Paulo Jannuzzi

    2001-01-01

    Full Text Available Trata-se de um caso de Transtorno de Personalidade Borderline, encaminhado para a área de Psicoterapia Dinâmica Breve (PDB do Centro de Psicologia Aplicada da UNESP - Bauru/SP. O foco delimitado consistiu em trabalhar as características depressivas do paciente, buscando ajudá-lo a elaborar o luto pela perda de sua mãe. A análise deste caso veio corroborar a hipótese de que pacientes com transtorno de personalidade borderline também podem ser beneficiados com a PDB. A peculiaridade desta modalidade de atendimento está relacionada ao estabelecimento de objetivos terapêuticos (foco condizentes com as reais possibilidades e limites de cada paciente.

  16. Benefits of Bifidobacterium breve M-16V Supplementation in Preterm Neonates - A Retrospective Cohort Study.

    Directory of Open Access Journals (Sweden)

    Sanjay K Patole

    Full Text Available Systematic reviews of randomised controlled trials report that probiotics reduce the risk of necrotising enterocolitis (NEC in preterm neonates.To determine whether routine probiotic supplementation (RPS to preterm neonates would reduce the incidence of NEC.The incidence of NEC ≥ Stage II and all-cause mortality was compared for an equal period of 24 months 'before' (Epoch 1 and 'after' (Epoch 2 RPS with Bifidobacterium breve M-16V in neonates <34 weeks. Multivariate logistic regression analysis was conducted to adjust for relevant confounders.A total of 1755 neonates (Epoch I vs. II: 835 vs. 920 with comparable gestation and birth weights were admitted. There was a significant reduction in NEC ≥ Stage II: 3% vs. 1%, adjusted odds ratio (aOR = 0.43 (95%CI: 0.21-0.87; 'NEC ≥ Stage II or all-cause mortality': 9% vs. 5%, aOR = 0.53 (95%CI: 0.32-0.88; but not all-cause mortality alone: 7% vs. 4%, aOR = 0.58 (95% CI: 0.31-1.06 in Epoch II. The benefits in neonates <28 weeks did not reach statistical significance: NEC ≥ Stage II: 6% vs. 3%, aOR 0.51 (95%CI: 0.20-1.27, 'NEC ≥ Stage II or all-cause mortality', 21% vs. 14%, aOR = 0.59 (95%CI: 0.29-1.18; all-cause mortality: 17% vs. 11%, aOR = 0.63 (95%CI: 0.28-1.41. There was no probiotic sepsis.RPS with Bifidobacterium breve M-16V was associated with decreased NEC≥ Stage II and 'NEC≥ Stage II or all-cause mortality' in neonates <34 weeks. Large sample size is required to assess the potential benefits of RPS in neonates <28 weeks.

  17. Efficacy of Bifidobacterium breve Fermented Milk in Maintaining Remission of Ulcerative Colitis.

    Science.gov (United States)

    Matsuoka, Katsuyoshi; Uemura, Yukari; Kanai, Takanori; Kunisaki, Reiko; Suzuki, Yasuo; Yokoyama, Kaoru; Yoshimura, Naoki; Hibi, Toshifumi

    2018-02-15

    Fermented milk products containing Bifidobacterium breve strain Yakult (BFM) may improve clinical status in ulcerative colitis (UC) patients. To assess efficacy of BFM in maintaining remission in Japanese patients with quiescent UC. This double-blind study (B-FLORA) enrolled 195 patients with quiescent UC, randomized to receive one pack of BFM fermented milk per day [Bifidobacterium breve strain Yakult (10 billion bacteria) and Lactobacillus acidophilus (1 billion bacteria)] (n = 98) or matching placebo (n = 97) for 48 weeks. The primary efficacy endpoint was relapse-free survival (relapse: rectal bleeding score ≥ 2 on Sutherland disease activity index scale for 3 consecutive days and/or initiation of remission induction therapy for worsening of UC). An interim analysis was conducted after inclusion and follow-up of one-third of patients for the first phase of the study (n = 195). Relapse-free survival was not significantly different between the BFM and placebo groups (P = 0.643; hazard ratio 1.16; 95% CI 0.63-2.14, log-rank test), nor was the incidence of relapse. Therefore, the study was discontinued for lack of efficacy. An exploratory analysis of fecal samples from a subgroup of patients revealed no effects of either study beverage on intestinal microbiota, but there was a significant decrease in Bifidobacterium species before relapse, regardless of treatment group. Three mild adverse events occurred for which a causal relationship with the study beverage could not be ruled out (placebo: abdominal bloating and stress in one patient; BFM: body odor in one patient). BFM had no effect on time to relapse in UC patients compared with placebo. UMIN000007593.

  18. Estado da Arte nos Quitinozoários: Designação da Ordem, Afinidades Biológicas, Paleoambiente e Breve Histórico no Âmbito das Bacias do Amazonas e do Paraná

    Directory of Open Access Journals (Sweden)

    Hilda Leonor Coevas de Azevedo-Soares

    2005-01-01

    Full Text Available Interpretados sob diversas óticas variando conforme o pensamento científico dos pesquisadores, os quitinozoários constituem um grupo enigmático tanto no que diz respeito ao seu significado como as suas afinidades biológicas. Distribuídos desde o Ordoviciano (Tremadociano até o final Devoniano, estes organismos são hoje ferramentas primordiais na datação desses estratos assim como na prospecção de depósitos de combustíveis fósseis. O presente trabalho levantará, portanto, aspectos da atualidade sobre o estado da arte dos quitinozoários tendo como base à designação da ordem Chitinozoa criada por A. Eisenack em 1931 e posteriormente reformulada pelo mesmo em 1972, com a divisão nas ordens Prosomatifera e Operculifera. Além de suas possíveis afinidades biológicas (filogenia com outros grupos fósseis e viventes como rotíferos e cnidários. No Brasil estes ocorrem desde o final do Ordoviciano? ao Devoniano, desta feita traçaremos um breve histórico sobre os trabalhos desenvolvidos nos últimos cinqüenta anos em duas de suas principais bacias paleozóicas: Amazonas e Paraná.

  19. Una reflexión crítica desde la agroecología sobre el dialogo de saberes, como necesidad impostergable en la formación de los ingenieros agrónomos

    OpenAIRE

    Larrañaga, Gustavo

    2015-01-01

    El presente trabajo es una reflexión crítica desde la Agroecologia sobre el dialogo de saberes desarrollado por nuestros profesionales universitarios, destacando esta práctica como necesidad impostergable en la formación de los Ingenieros Agrónomos, partiendo de un breve diagnóstico sobre la práctica de enseñanza universitaria, los diseños curriculares existentes y la formación de nuestros profesionales. Se analiza el posicionamiento generalizado de nuestros profesionales, frecuentemente auto...

  20. Bifidobacterium breve reduces apoptotic epithelial cell shedding in an exopolysaccharide and MyD88-dependent manner.

    Science.gov (United States)

    Hughes, K R; Harnisch, L C; Alcon-Giner, C; Mitra, S; Wright, C J; Ketskemety, J; van Sinderen, D; Watson, A J M; Hall, L J

    2017-01-01

    Certain members of the microbiota genus Bifidobacterium are known to positively influence host well-being. Importantly, reduced bifidobacterial levels are associated with inflammatory bowel disease (IBD) patients, who also have impaired epithelial barrier function, including elevated rates of apoptotic extrusion of small intestinal epithelial cells (IECs) from villi-a process termed 'cell shedding'. Using a mouse model of pathological cell shedding, we show that mice receiving Bifidobacterium breve UCC2003 exhibit significantly reduced rates of small IEC shedding. Bifidobacterial-induced protection appears to be mediated by a specific bifidobacterial surface exopolysaccharide and interactions with host MyD88 resulting in downregulation of intrinsic and extrinsic apoptotic responses to protect epithelial cells under highly inflammatory conditions. Our results reveal an important and previously undescribed role for B. breve, in positively modulating epithelial cell shedding outcomes via bacterial- and host-dependent factors, supporting the notion that manipulation of the microbiota affects intestinal disease outcomes. © 2017 The Authors.

  1. Overexpression, crystallization and preliminary X-ray analysis of xylulose-5-phosphate/fructose-6-phosphate phosphoketolase from Bifidobacterium breve

    International Nuclear Information System (INIS)

    Suzuki, Ryuichiro; Kim, Byung-Jun; Shibata, Tsuyoshi; Iwamoto, Yuki; Katayama, Takane; Ashida, Hisashi; Wakagi, Takayoshi; Shoun, Hirofumi; Fushinobu, Shinya; Yamamoto, Kenji

    2010-01-01

    Xylulose-5-phosphate/fructose-6-phosphate phosphoketolase from B. breve was overexpressed and crystallized. The crystals belonged to the tetragonal space group I422 and diffracted to beyond 1.7 Å resolution. The xylulose-5-phosphate/fructose-6-phosphate phosphoketolase gene from Bifidobacterium breve was cloned and overexpressed in Escherichia coli. The enzyme was purified to homogeneity and crystallized by the sitting-drop vapour-diffusion method. Crystals were obtained at 293 K using 0.05 mM thiamine diphosphate, 0.25 mM MgCl 2 , 24%(w/v) PEG 6000 and 0.1 M Bicine pH 9.0. The crystals belonged to the tetragonal space group I422, with unit-cell parameters a = b = 174.8, c = 163.8 Å, and diffracted to beyond 1.7 Å resolution

  2. Transcriptional and functional characterization of genetic elements involved in galacto-oligosaccharide utilization by Bifidobacterium breve UCC2003.

    Science.gov (United States)

    O'Connell Motherway, Mary; Kinsella, Michael; Fitzgerald, Gerald F; van Sinderen, Douwe

    2013-01-01

    Several prebiotics, such as inulin, fructo-oligosaccharides and galacto-oligosaccharides, are widely used commercially in foods and there is convincing evidence, in particular for galacto-oligosaccharides, that prebiotics can modulate the microbiota and promote bifidobacterial growth in the intestinal tract of infants and adults. In this study we describe the identification and functional characterization of the genetic loci responsible for the transport and metabolism of purified galacto-oligosaccharides (PGOS) by Bifidobacterium breve UCC2003. We further demonstrate that an extracellular endogalactanase specified by several B. breve strains, including B. breve UCC2003, is essential for partial degradation of PGOS components with a high degree of polymerization. These partially hydrolysed PGOS components are presumed to be transported into the bifidobacterial cell via various ABC transport systems and sugar permeases where they are further degraded to galactose and glucose monomers that feed into the bifid shunt. This work significantly advances our molecular understanding of bifidobacterial PGOS metabolism and its associated genetic machinery to utilize this prebiotic. © 2012 The Authors. Published by Society for Applied Microbiology and Blackwell Publishing Ltd. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

  3. Autoinducer-2 plays a crucial role in gut colonization and probiotic functionality of Bifidobacterium breve UCC2003.

    Science.gov (United States)

    Christiaen, Steven E A; O'Connell Motherway, Mary; Bottacini, Francesca; Lanigan, Noreen; Casey, Pat G; Huys, Geert; Nelis, Hans J; van Sinderen, Douwe; Coenye, Tom

    2014-01-01

    In the present study we show that luxS of Bifidobacterium breve UCC2003 is involved in the production of the interspecies signaling molecule autoinducer-2 (AI-2), and that this gene is essential for gastrointestinal colonization of a murine host, while it is also involved in providing protection against Salmonella infection in Caenorhabditis elegans. We demonstrate that a B. breve luxS-insertion mutant is significantly more susceptible to iron chelators than the WT strain and that this sensitivity can be partially reverted in the presence of the AI-2 precursor DPD. Furthermore, we show that several genes of an iron starvation-induced gene cluster, which are downregulated in the luxS-insertion mutant and which encodes a presumed iron-uptake system, are transcriptionally upregulated under in vivo conditions. Mutation of two genes of this cluster in B. breve UCC2003 renders the derived mutant strains sensitive to iron chelators while deficient in their ability to confer gut pathogen protection to Salmonella-infected nematodes. Since a functional luxS gene is present in all tested members of the genus Bifidobacterium, we conclude that bifidobacteria operate a LuxS-mediated system for gut colonization and pathogen protection that is correlated with iron acquisition.

  4. Autoinducer-2 plays a crucial role in gut colonization and probiotic functionality of Bifidobacterium breve UCC2003.

    Directory of Open Access Journals (Sweden)

    Steven E A Christiaen

    Full Text Available In the present study we show that luxS of Bifidobacterium breve UCC2003 is involved in the production of the interspecies signaling molecule autoinducer-2 (AI-2, and that this gene is essential for gastrointestinal colonization of a murine host, while it is also involved in providing protection against Salmonella infection in Caenorhabditis elegans. We demonstrate that a B. breve luxS-insertion mutant is significantly more susceptible to iron chelators than the WT strain and that this sensitivity can be partially reverted in the presence of the AI-2 precursor DPD. Furthermore, we show that several genes of an iron starvation-induced gene cluster, which are downregulated in the luxS-insertion mutant and which encodes a presumed iron-uptake system, are transcriptionally upregulated under in vivo conditions. Mutation of two genes of this cluster in B. breve UCC2003 renders the derived mutant strains sensitive to iron chelators while deficient in their ability to confer gut pathogen protection to Salmonella-infected nematodes. Since a functional luxS gene is present in all tested members of the genus Bifidobacterium, we conclude that bifidobacteria operate a LuxS-mediated system for gut colonization and pathogen protection that is correlated with iron acquisition.

  5. Fiabilidad del Instrumento de Evaluación Neuropsicológica Breve Neupsilin

    Directory of Open Access Journals (Sweden)

    Josiane Pawlowski

    2013-01-01

    Full Text Available La investigación examinó la fiabilidad del Instrumento de Evaluación Neuropsicológica Breve Neupsilin, desarrollado en Brasil. Participaron 102 hombres y mujeres brasileños, de 18 a 40 años de edad. Se evaluó la fiabilidad del test-retest del Neupsilin y de la corrección, por distintos evaluadores, de la prueba para evaluación de apraxia constructiva. Se analizaron los datos con: el test de correlación de Spearman, el coeficiente de correlación intraclase y el coeficiente alpha de Cronbach. Las habilidades de lenguaje, memoria, praxias y funciones ejecutivas presentaron las más altas correlaciones para los resultados del test-retest. Se encontró una concordancia de moderada a alta entre los correctores de la tarea de praxia constructiva. Los resultados indicaron fiabilidad temporal para las tareas evaluadas por el Neupsilin y fiabilidad del corrector para la tarea praxia constructiva. Se presentan sugerencias para perfeccionar las tareas y mejorar la fiabilidad y la validez de la prueba.

  6. Fragmentos, fractales y fronteras: género y lectura en las series de narrativa Breve

    Directory of Open Access Journals (Sweden)

    Zavala, Lauro

    2004-06-01

    Full Text Available In this paper I study some strategies for reading narrative series that enable the reader to play with the conventional boundaries between textual unity (in particular, that of the novel and generic diversity (in particular, that of the short and the short-short story. These strategies enable the reader to distinguish between short story cycles, short-short story cycles, fragmentary novel, and dispersed stories. What is at stake is a new aesthetics of reading which produces a re-statement of the very boundaries between the whole and its parts.En este trabajo señalo la existencia de varias estrategias de escritura, edición y lectura de series narrativas que permiten relativizar las fronteras convencionales entre la unidad textual (en particular, la unidad de la novela y la diversidad genérica (en particular, la identidad de cada cuento o minificción. Estas estrategias son las que permiten reconocer las diferencias entre cuentos integrados, novela fragmentaria, minificciones integradas, ciclos de minificción y cuentos dispersos. Lo que está en juego en el estudio de las series de textos breves es el reconocimiento de las posibilidades de lectura que ofrecen las manifestaciones textuales de lo mismo y lo múltiple, en la reformulación de las fronteras entre el todo y las partes.

  7. Mechanisms involved in alleviation of intestinal inflammation by bifidobacterium breve soluble factors.

    Directory of Open Access Journals (Sweden)

    Elise Heuvelin

    Full Text Available OBJECTIVES: Soluble factors released by Bifidobacterium breve C50 (Bb alleviate the secretion of pro-inflammatory cytokines by immune cells, but their effect on intestinal epithelium remains elusive. To decipher the mechanisms accounting for the cross-talk between bacteria/soluble factors and intestinal epithelium, we measured the capacity of the bacteria, its conditioned medium (Bb-CM and other Gram(+ commensal bacteria to dampen inflammatory chemokine secretion. METHODS: TNFalpha-induced chemokine (CXCL8 secretion and alteration of NF-kappaB and AP-1 signalling pathways by Bb were studied by EMSA, confocal microscopy and western blotting. Anti-inflammatory capacity was also tested in vivo in a model of TNBS-induced colitis in mice. RESULTS: Bb and Bb-CM, but not other commensal bacteria, induced a time and dose-dependent inhibition of CXCL8 secretion by epithelial cells driven by both AP-1 and NF-kappaB transcription pathways and implying decreased phosphorylation of p38-MAPK and IkappaB-alpha molecules. In TNBS-induced colitis in mice, Bb-CM decreased the colitis score and inflammatory cytokine expression, an effect reproduced by dendritic cell conditioning with Bb-CM. CONCLUSIONS: Bb and secreted soluble factors contribute positively to intestinal homeostasis by attenuating chemokine production. The results indicate that Bb down regulate inflammation at the epithelial level by inhibiting phosphorylations involved in inflammatory processes and by protective conditioning of dendritic cells.

  8. Bifidobacterium breve alters immune function and ameliorates DSS-induced inflammation in weanling rats.

    Science.gov (United States)

    Izumi, Hirohisa; Minegishi, Mario; Sato, Yohei; Shimizu, Takashi; Sekine, Kazunori; Takase, Mitsunori

    2015-10-01

    Bifidobacterium breve M-16V (M16V) is a probiotic bacterial strain with a long tradition of use in neonatal intensive care units in some countries. Previous study showed that the effects of M16V administration on gene expression were greater during the weaning period than in the neonatal period and were greater in the colon than in the small intestine and spleen, suggesting that M16V has anti-inflammatory effects. In this study, we evaluated the effects of inflammation during the weaning period and the effects of M16V on normal and inflammatory conditions. From postnatal day (PD) 21 to 34, weanling rats were administered of 2.5 × 10(9) of M16V daily, and colitis was induced by administration of 2% dextran sulfate sodium from PD28 to 35. Colitis severity, immune function, and microbiota were investigated. Colitis caused a reduction in body weight gain, colon shortening, poor nutritional status, anemia, changes in blood and spleen lymphocyte populations, spleen T-cell malfunctions, and alterations in colon microbiota. M16V administration improved some but not all of the changes induced by colitis. M16V could suppress inflammation and, therefore, can be considered a safe strain to use not only during the neonatal period but also the weaning period.

  9. Sensory characteristics and volatile composition of a cereal beverage fermented with Bifidobacterium breve NCIMB 702257.

    Science.gov (United States)

    Salmerón, Ivan; Rozada, Raquel; Thomas, Keith; Ortega-Rivas, Enrique; Pandiella, Severino S

    2014-04-01

    Most of the commercialized lactic acid fermented products are dairy-based. Hence, the development of non-dairy fermented products with probiotic properties draws significant attention within the functional foods industry. The microorganisms used in such products have complex enzyme systems through which they generate diverse metabolites (volatile and non-volatile) that provide significant flavour attributes of importance for fermented foods. The correlation of the volatile flavour compounds of a malt beverage fermented with a Bifidobacterium breve strain with its unique sensory characteristics was performed. The volatile composition analysis exposed the presence of 12 components. Eight of these flavour volatiles were produced through the metabolic activity of the bifidobacteria strain. Notably acetic acid, of reported sour flavour characteristics, exhibited the greatest intensity. Four components of considerable organoleptic characteristics were identified as Maillard-derived products, namely maltol, pyranone, 2 (5H)-furanmethanol and 3-furanmethanol. The sensory evaluation exhibited that the fermented cereal beverage had a sour flavour with mild sweet and malty notes. These results indicate that the volatile compounds identified can be appointed as significant flavour markers of the novel fermented cereal beverage.

  10. Fermented inulin hydrolysate by Bifidobacterium breve as cholesterol binder in functional food application

    Science.gov (United States)

    Melanie, Hakiki; Susilowati, Agustine; Maryati, Yati

    2017-01-01

    Inulin hydrolysate is a result of inulin hydrolysis by inulinase enzyme of Scopulariopsis sp.-CBS1 fungi isolated from dahlia tuber skin in the formation of fructooligosaccharides (FOS) as dietary fiber. Inulin hydrolysate fermented by Bifidobacterium breve has a potential as cholesterol binder in digestive system due to dietary fiber content in inulin. This study was conducted to evaluate the best cholesterol binding capacity by the variation of lactic acid bacteria (LAB) culture concentration of 10%, 20% and 30% (v/v), respectively. Fermentation process were conducted with inulin hydrolysate concentration of 25% (w/v), skim milk 7,5% (w/v) and various LAB culture concentration at 40 °C for 0, 12, 24, 36 and 48 hours. The results showed that the variation of LAB culture concentrations affect the cholesterol binding ability in fermented inulin hydrolysate. The fermentation process with 10% LAB culture concentration at 40°C for 48 hours resulted in the highest cholesterol binding capacity (CBC) of 13,69 mg/g at pH 7and 14,44 mg/g at pH 2 with composition of total acids of 0,787%, soluble dietary fiber of 0,396%, insoluble dietary fiber of 5,47%, total solids of 14,476%, total sugars of 472,484 mg/mL, reducing sugar of 92 mg/mL and total plate count (TPC) of 7,278 log CFU/mL, respectively.

  11. Less is more: bondades de lo breve en el Río de la Plata

    Directory of Open Access Journals (Sweden)

    Julio Prieto

    2014-06-01

    Full Text Available Las famosas palabras del prólogo de El jardín de senderos que se bifurcan condensan una poética narrativa que no sólo podemos considerar emblemática de uno de los autores seminales del siglo XX –Jorge Luis Borges– sino que también informa una de las líneas de escritura más fructíferas de la literatura del Río de la Plata: aquella que cifra un decoro literario en la eficacia de la brevedad. En esa tradición se cuentan algunas de las obras más significativas de esa literatura, desde la microtextualidad de los prólogos, “resúmenes y comentarios” de Borges, a los relatos breves y viñetas humorísticas de Julio Cortázar, Cristina Peri Rossi, Luisa Valenzuela y Ana María Shua.

  12. Effect of Gymnodinium breve toxin in the rat phrenic nerve diaphragm preparation.

    Science.gov (United States)

    Gallagher, J. P.; Shinnick-Gallagher, P.

    1980-01-01

    1 The effects of a crude fraction of Gymnodinium breve toxin (GBTX) were studied on the rat phrenic nerve diaphragm preparation. 2 Indirectly stimulated muscle contractions were more sensitive to blockade by increasing concentrations of GBTX (0.25 microgram/ml to 10 micrograms/ml) than direct muscle contractions. 3 GBTX increased miniature endplate potential (m.e.p.p.) frequency and depolarized the resting membrane potential of the muscle fibres at the endplate and in non-synaptic regions of muscle fibre. 4 A concentration-dependent biphasic effect on m.e.p.p. amplitude was evident. At lower concentrations m.e.p.p. amplitudes were depressed whereas at high concentrations they were increased. 5 GBTX blocked endplate potentials (e.p.ps) in concentrations (0.5 microgram/ml to 2 micrograms/ml) that did not block m.e.p.ps. 6 Muscle fibre action potentials recorded from cells held at control membrane potential by hyperpolarizing current were not altered by toxin treatment. No repetitive e.p.ps, or muscle action potentials were observed. 7 These data suggest that GBTX may block indirectly stimulated muscle contractions and e.p.ps by depolarizing the nerve terminal in a manner similar to the observed depolarization of the muscle fibre. The depolarization of the nerve terminal may be sufficient to inhibit transmitter release. PMID:7190451

  13. Central respiratory and circulatory effects of Gymnodinium breve toxin in anaesthetized cats

    Science.gov (United States)

    Borison, Herbert L.; Ellis, Sydney; McCarthy, Lawrence E.

    1980-01-01

    1 In cats anaesthetized with pentobarbitone, observations were made on respiration, spontaneous and evoked diaphragmatic electromyograms, blood pressure, heart rate, indirectly-induced contractions of the anterior tibialis muscle and nictitating membrane, and electrical excitability of the inspiratory centre in the medulla oblongata. 2 Gymnodinium breve toxin (GBTX) was administered intravenously, intra-arterially to the brain, and intracerebroventricularly. Physiological effects were recorded while alveolar PCO2 was controlled at a constant level except when changes in gas tension were made in order to measure CO2-ventilatory responsiveness. 3 Adequate doses of GBTX given intravenously by bolus injection elicited a non-tachyphylactic reflex response triad of apnoea, hypotension and bradycardia mediated by the vagus nerves independently of arterial baroreceptor and chemoreceptor innervation. 4 After vagotomy, additional amounts of GBTX (i.v.) resulted in apneustic breathing, hypertension and tachycardia. The cardiovascular effects were abolished by ganglionic blockade with hexamethonium. 5 Smaller doses of GBTX were required intra-arterially and intracerebroventricularly than by the intravenous route of injection to produce respiratory irregularity and cardiovascular hyperactivity. 6 Evoked motor responses, electrical excitability of the medulla oblongata and CO2-ventilatory responsiveness were largely spared even though GBTX caused marked disturbances in respiratory rhythmicity and cardiovascular functions. 7 It is concluded that GBTX acts reflexly on vagally innervated receptors to evoke a Bezold-Jarisch effect but that the toxin further acts centrally to cause irregular breathholding and hypertension with tachycardia, leading ultimately to respiratory and circulatory failure. PMID:7191740

  14. Possible mechanisms of action of Gymnodinium breve toxin at the mammalian neuromuscular junction.

    Science.gov (United States)

    Shinnick-Gallagher, P.

    1980-01-01

    1 The mechanism of action of a crude fraction of Gymnodinium breve toxin (GBTX) was investigated by intracellular recording techniques in the rat phrenic nerve diaphragm preparation. 2 GBTX (2 micrograms/ml) decreased the input resistance of the muscle membrane concomitantly with a depolarization of the resting membrane potential. 3 A low sodium solution reversed or prevented a GBTX-induced membrane depolarization. 4 Tetrodotoxin (TTX) antagonized a GBTX-induced increase in miniature endplate potential (m.e.p.p.) frequency and repolarized a GBTX-depolarized membrane. Pretreatment with TTX prevented GBTX effects. 5 GBTX reversibly reduced depolarizations produced by bath applied acetylcholine (ACh). The membrane depolarization was not responsible for the depression of ACh responses. 6 These findings suggest that GBTX increases m.e.p.p. frequency and depolarizes the resting membrane potential by increasing sodium permeability. The reduction of ACh-induced depolarizations suggests that GBTX may be acting at some site on the ACh receptor. PMID:7190452

  15. A Gene Homologous to rRNA Methylase Genes Confers Erythromycin and Clindamycin Resistance in Bifidobacterium breve.

    Science.gov (United States)

    Martínez, Noelia; Luque, Roberto; Milani, Christian; Ventura, Marco; Bañuelos, Oscar; Margolles, Abelardo

    2018-05-15

    Bifidobacteria are mutualistic intestinal bacteria, and their presence in the human gut has been associated with health-promoting activities. The presence of antibiotic resistance genes in this genus is controversial, since, although bifidobacteria are nonpathogenic microorganisms, they could serve as reservoirs of resistance determinants for intestinal pathogens. However, until now, few antibiotic resistance determinants have been functionally characterized in this genus. In this work, we show that Bifidobacterium breve CECT7263 displays atypical resistance to erythromycin and clindamycin. In order to delimit the genomic region responsible for the observed resistance phenotype, a library of genomic DNA was constructed and a fragment of 5.8 kb containing a gene homologous to rRNA methylase genes was able to confer erythromycin resistance in Escherichia coli This genomic region seems to be very uncommon, and homologs of the gene have been detected in only one strain of Bifidobacterium longum and two other strains of B. breve In this context, analysis of shotgun metagenomics data sets revealed that the gene is also uncommon in the microbiomes of adults and infants. The structural gene and its upstream region were cloned into a B. breve -sensitive strain, which became resistant after acquiring the genetic material. In vitro conjugation experiments did not allow us to detect gene transfer to other recipients. Nevertheless, prediction of genes potentially acquired through horizontal gene transfer events revealed that the gene is located in a putative genomic island. IMPORTANCE Bifidobacterium breve is a very common human intestinal bacterium. Often described as a pioneer microorganism in the establishment of early-life intestinal microbiota, its presence has been associated with several beneficial effects for the host, including immune stimulation and protection against infections. Therefore, some strains of this species are considered probiotics. In relation to this

  16. APROXIMACIÓN AL CONCEPTO DE GOBERNANZA EN COLOMBIA Y ALGUNOS APUNTES SOBRE SU IMPORTANCIA EN EL DERECHO AMBIENTAL

    Directory of Open Access Journals (Sweden)

    Erika Castro Buitrago

    2011-12-01

    Full Text Available Este artículo presenta un breve estudio sobre la recepción del concepto de Gobernanza en Colombia, su uso en la política ambiental y la investigación interna que existe en esta materia. En especial, el artículo llama la atención sobre la necesidad de estudiar la influencia del término en el ámbito del derecho administrativo ambiental, a partir de la importancia que otros ordenamientos jurídicos le han otorgado. En el caso europeo, la gobernanza se entiende como el modelo ideal de gobernación para gestionar los asuntos ambientales

  17. Inferencias sobre Grafos

    OpenAIRE

    Sira M. Allende; Carlos N. Bouza

    2002-01-01

    El estudio de un juego puede ser modelado asumiendo que solo algunas partidas son observadas. Entonces el árbol del juego debe ser estimado utilizando información muestral. Similarmente ocurre al obtener información sobre el comportamiento de las decisiones tomadas por individuos muestreados sobre un árbol de decisión teórico. Al considerar una medida de probabilidad que caracterice el comportamiento de redes aleatorias se puede obtener un estimado del árbol asociado. Este problema es el anal...

  18. Sobre software libre

    OpenAIRE

    Matellán Olivera, Vicente; González Barahona, Jesús; Heras Quirós, Pedro de las; Robles Martínez, Gregorio

    2004-01-01

    220 p. "Sobre software libre" reune casi una treintena de ensayos sobre temas de candente actualidad relacionados con el software libre (del cual Linux es su ex- ponente más conocido). Los ensayos que el lector encontrará están divididos en bloques temáticos que van desde la propiedad intelectual o las cuestiones económicas y sociales de este modelo hasta su uso en la educación y las administraciones publicas, pasando por alguno que repasa la historia del software libre en l...

  19. The early impact of therapeutic alliance in brief psychodynamic psychotherapy O impacto inicial da aliança terapêutica em psicoterapia psicodinâmica breve

    Directory of Open Access Journals (Sweden)

    José Alvaro Marques Marcolino

    2003-06-01

    Full Text Available INTRODCTION: Therapeutic alliance is a key component of the psychotherapeutic process. This study estimated the impact of the therapeutic alliance as measured by CALPAS-P in an individual brief psychodynamic psychotherapy program. METHODS: To study the impact of the therapeutic alliance patients in psychotherapy answered to the CALPAS-P at the first and third session and to the Self-report Questionnaire (SRQ-20, to the Beck Depression Inventory (BDI and to the Hamilton Anxiety Scale at the beginning and at the end of psychotherapy. RESULTS: The study of the impact of the therapeutic alliance in brief psychodynamic psychotherapy showed that higher TUI scores in the first session were significantly associated to the improvement on the BDI. Patients with best scores in the working alliance, measured at the third PWC session had also significant symptomatic changes. DISCUSSION: The study of the impact of the therapeutic alliance in brief psychotherapy indicated that patients who perceived that their therapists had the best capability to understand and to be involved in their issues had best results in reducing depressive symptoms and patients with higher capability to form the working alliance reached the best psychotherapy outcomes.INTRODUÇÃO: A aliança terapêutica é um conceito central do processo psicoterápico. Este estudo avaliou o impacto da aliança terapêutica em um programa de psicoterapia individual psicodinâmica breve. MÉTODO: Para o estudo do impacto da aliança, pacientes em psicoterapia responderam, ao início e ao final de cada psicoterapia, ao Questionário de auto-avaliação (SRQ-20, ao Inventário de Depressão de Beck (BDI e à Escala de Ansiedade de Hamilton. Responderam também a CALPAS-P ao término da primeira e da terceira sessão. RESULTADOS: Os resultados mostraram que os pacientes com uma pontuação mais alta da TUI na primeira sessão tiveram um impacto significativo sobre a mudança da sintomatologia medida

  20. Psicoterapia breve psicodinâmica preventiva: pesquisa exploratória de resultados e acompanhamento Psicoterapia breve psicodinamica preventiva: pesquisa exploratoria de resultados y acompañamiento Brief preventive psychodynamic psychotherapy: exploratory research on results and follow-up

    Directory of Open Access Journals (Sweden)

    Tales Vilela Santeiro

    2008-12-01

    Full Text Available Há escassez de estudos sistemáticos sobre processos de psicoterapia nas clínicas-escola brasileiras, inclusive os de caráter preventivo. A proposta focaliza Psicoterapias Breves Psicodinâmicas (PBPs conduzidas por estudantes em contexto de clínica-escola. Tem como objetivo avaliar os graus de depressão e ansiedade e o neuroticismo em voluntários, estudantes de curso de psicologia (N=6, no início, no final e após seis meses do término das PBP (follow-up. As Escalas Beck de Depressão e Ansiedade (BD/BAI e de Ajustamento Emocional/Neuroticismo foram os instrumentos empregados. As sessões foram semanais, num total de 10, gravadas em áudio e observadas em sala de espelho unidirecional. De modo geral foram constatadas mudanças em relação às variáveis analisadas, o que ficou mais evidente do início à 10ª sessão e quando as escalas BDI e BAI foram utilizadas como medidas. Os eventuais ganhos obtidos quando do término nem sempre foram mantidos no follow-up.Hay escasez de estudios sistemáticos sobre los procesos de psicoterapia en las clínicas-escuelas brasileñas, incluso los estudios de carácter preventivo. La propuesta focaliza psicoterapias breves psicodinámicas (PBP conducidas en contexto de clínica-escuela. Este estudio tiene como objetivos evaluar los grados de depresión y ansiedad y el neuroticismo en voluntarios, estudiantes del curso de psicología (N=6, en el inicio, en el final y después de seis meses del término de las PBP (follow-up. Las Escalas Beck de Depresión y Ansiedad (BD/BAI y de Ajustamiento Emocional/Neuroticismo fueron semanales, totalizando diez, grabadas en audio y observadas en sala de espejo unidireccional. De un modo general fueron constatados cambios en relación a las variables analizadas, lo que dejó más evidente del inicio a la décima sesión y cuando las escalas BDI y BAI fueron utilizadas como medidas. Los eventuales logros obtenidos en el término no siempre fueron mantenidos en el

  1. La ficción en Internet para trasladar información de actualidad: ¿Diga?, un cortometraje sobre la huella ecológica

    OpenAIRE

    Velasco Manso, Marta

    2017-01-01

    ¿Diga? es un cortometraje de ficción que traslada información sobre hábitos de consumo responsable con el fin de mostrar su importancia en el medio ambiente y en concreto, en la reducción de la huella ecológica. El cortometraje se enmarca dentro de un proyecto digital, Episodio Piloto, una página web donde albergar producciones breves de ficción junto a información que se pretenda mostrar con ellas. La información ligada a ¿Diga? se traslada mediante un artículo sencillo que explica el concep...

  2. Efectos de los ejercicios de Pilates sobre el volumen muscular en la región lumbo-pélvica

    OpenAIRE

    López Gordillo, Ana María

    2013-01-01

    Programa de doctorado: Actividad física, salud y rendimiento deportivo [ES] Esta tesis doctoral consta de tres partes. Una primera parte dedicada a la introducción donde se hace una breve referencia a los antecedentes históricos del Método Pilates. Una segunda parte de fundamentación teórica donde se describe la bibliografía científica relacionada con los estudios de investigación sobre el Método Pilates. Finalmente, la tercera parte dedicada al estudio de investigación cuyo objetivo fue d...

  3. Sobre la toma de decisiones económicas bajo incertidumbre

    Directory of Open Access Journals (Sweden)

    Aurora García Gallego

    2012-04-01

    Full Text Available En este artículo resumimos la evolución de la Teoría Económica sobre la toma de decisiones bajo incertidumbre. Tras una breve introducción a la teoría de la Utilidad Esperada, nos referimos a comportamientos paradójicos que obligaron a los economistas a adoptar teorías menos simplistas que permitieran explicar un mayor abanico de fenómenos. La mayor complejidad conlleva una descripción multidimensional de las actitudes humanas frente al riesgo económico. Sin embargo, las mediciones de la actitud frente al riesgo utilizadas por los economistas continúan siendo, en su mayoría, unidimensionales.

  4. A segunda regra fundamental: um comentário sobre o Ferenczi de Lacan

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    Angela Cavalcanti Bernardes

    2002-12-01

    Full Text Available Que o analista seja analisado é a segunda regra fundamental da psicanálise, de acordo com Sándor Ferenczi. Este autor é louvado por Lacan pelo questionamento autêntico de sua responsabilidade de terapeuta. Faz-se aqui um breve comentário de algumas referências de Lacan a Ferenczi, tendo-se por perspectiva o controverso debate sobre a formação do analista.The second fundamental rule: a note about Lacan's Ferenczi. The analysis of the analyst is the second rule of psychoanalysis, following Sándor Ferenczi. This author is considered by Lacan an authentic questioner of his responsibility as a psychotherapist. The reader will find here a brief note about some of Lacan's comments on Ferenczi. Our concern here is the training of the analyst.

  5. Museus e diversidade sexual: reflexões sobre mostras LGBT e QUEER

    Directory of Open Access Journals (Sweden)

    Renato Pinto

    2015-06-01

    Full Text Available Esta breve reflexão está focada no tema das exibições em museus que buscam mostrar artefatos, a história e a memória de grupos LGBT-Q (lésbicas, gays, bissexuais, transgêneros e queer. Interessa-me relatar – sem a pretensão de acentuado aprofundamento nas discussões teóricas ou de exaustiva apresentação de exemplos – algumas das inquietudes e soluções criativas que se apresentam aos curadores e organizadores de mostras que contenham acervo erótico ou de cunho sexual que possam ser associados aos grupos LGBT-Q, inclusive mostras sobre a Antiguidade. Parte desta reflexão é resultado de um trabalho que desenvolvo desde o inicio de meu doutoramento no campo da História Cultural.

  6. Podemos, de la invisibilidad a la sobre-exposición

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    Victor Sampedro

    2015-04-01

    Full Text Available El éxito comunicativo de Podemos es un ejemplo de cómo aplicar las teorías de lahegemonía ideológica que analizaron sus líderes académicos. Su estrategia mediática consiste en una combinación secuencial y simbiótica de producción audiovisual propia,viralización en redes digitales, y presencia en medios y formatos convencionales mayoritarios, como la tertulia televisiva. Sus limitaciones muestran la importancia de la economía política de los medios convencionales, aún mayoritarios entre la audiencia y decisivos para disputar la hegemonía. En un breve periodo de tiempo la joven formación política ha pasado de la invisibilidad a la sobre-exposición negativa.

  7. Conceptos generales sobre ABA en niños con trastorno del espectro autista

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    Sandra Elizabeth Piñeros Ortiz

    2012-01-01

    Full Text Available Los trastornos del espectro autista (TEA son entidades que generan discapacidad. Diversas intervenciones psicofarmacológicas y psicosociales modulan algunas de las alteraciones comportamentales asociadas y mejoran la calidad de vida de las personas afectadas y de sus cuidadores. La terapia ABA es una de las intervenciones psicosociales más conocidas y utilizada en población con TEA. Mediante de la  formulación de preguntas clave y sus respuestas, este artículo realiza una breve descripción de los aspectos históricos, las principales características y los fundamentos teóricos del ABA. Se discuten los resultados de diversos estudios que señalan las limitaciones metodológicas de las investigaciones sobre  la efectividad de esta terapia y sus implicaciones para la práctica clínica.

  8. USO DE ANIMALES DE LABORATORIO EN COLOMBIA: REFLEXIONES SOBRE ASPECTOS NORMATIVOS Y ÉTICOS

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    L. Botero

    2013-01-01

    Full Text Available El uso de modelos animales para la experimentación se ha incrementado considerablemente en Colombia. Mientras en países desarrollados estos animales se producen en ambientes controlados bajo normas estrictas que garantizan la validez de los resultados de investigación, en Colombia la normatividad al respecto es precaria e imprecisa lo que ha llevado a que pocas instituciones mantengan los animales con los estándares requeridos para obtener resultados de investigación con el rigor que la comunidad científica internacional exige. En este artículo presentamos una breve revisión de la normatividad en el tema a nivel mundial y, finalmente, se mencionan algunas recomendaciones sobre ajgunos aspectos que debería integrar la normatividad colombiana para que se adecúe a las regulaciones internacionales.

  9. Educação e ensino de História: a visão da cronista Rachel de Queiroz sobre o pós-guerra e a situação da mulher na Europa

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    Regma Maria Santos

    2013-07-01

    Full Text Available Este texto tem como objetivo apresentar a possibilidade de utilizar, no ensino de História, a crônica escrita por Rachel de Queiroz em jornais e revistas da primeira metade do século XX. Apresentamos antes uma breve biografia da autora, enfocando o contexto de sua produção, e depois realizamos uma breve análise da crônica selecionada, sinalizando oportunidades para trabalhar com os estudantes. Este documento oferece uma leitura pessoal sobre certos temas e também uma percepção histórica mais ampla e mais crítica do mundo.

  10. Bifidobacterium longum and Bifidobacterium breve isolates from preterm and full term neonates: comparison of cell surface properties.

    Science.gov (United States)

    Andriantsoanirina, Valérie; Teolis, Anne-Claire; Xin, Liu Xin; Butel, Marie Jose; Aires, Julio

    2014-08-01

    We compared autoaggregation, surface hydrophobicity and Caco-2 cells adhesion capabilities of independent Bifidobacterium breve (n = 22) and Bifidobacterium longum (n = 25) strains isolated from preterm (n = 20) and full term neonates (n = 27). Concerning strains properties, a correlation between autoaggregation and surface hydrophobicity was found for B. longum (r = 0.40, p = 0.048), B. breve (r = 0.57, p = 0.005), and all strains independently of the species consideration (r = 0.46, p = 0.001). The absence of difference in adhesion capabilities between preterm and full term neonate strains suggests a strain-dependent property. However, B. longum strains from preterm neonates (n = 10) showed higher autoaggregation ability (p = 0.044). Additionally, independently of species consideration, preterm neonates strains showed lower surface hydrophobicity (p = 0.027). As far as species are considered, preterm neonate B. breve strains (n = 10) showed significantly lower surface hydrophobicity percentages (p = 0.043). Our results suggest the existence of variations in bifidobacteria membrane structure and/or composition that may reflect adaptation of these bacteria to the intestinal environment of either preterm or full term neonates. Such information is of interest when considering the use of bifidobacteria probiotic strains for modulation of preterm neonates gut microbiota. Copyright © 2014 Elsevier Ltd. All rights reserved.

  11. Prevalence of HCV infection and associated factors among illicit drug users in Breves, State of Pará, northern Brazil

    OpenAIRE

    Pacheco,Suzy Danielly Barbosa; Silva-Oliveira,Gláucia Caroline; Maradei-Pereira,Luciana Maria Cunha; Crescente,José Ângelo Barletta; Lemos,José Alexandre Rodrigues de; Oliveira-Filho,Aldemir Branco de

    2014-01-01

    Introduction: Illicit drug users (DUs) are vulnerable to hepatitis C virus (HCV) infection. The shared use of illicit drugs is the main method of HCV transmission. Methods: A cross-sectional study was conducted in Breves, in northern Brazil. We surveyed 187 DUs to determine the prevalence of and factors associated with HCV infection. Results: The prevalence of anti-HCV antibodies was 36.9%, and the prevalence of hepatitis C virus-ribonucleic acid (HCV-RNA) was 31%. Hepatitis C virus infec...

  12. Critical micelle concentrations of allelopathic substances produced by Nannochloris oculata which affect a red tide organism, Gymnodinium breve.

    Science.gov (United States)

    Pérez, E; Martin, D F

    2001-01-01

    Laboratory cultures of the green algae Nannochloris oculata and Nannochloris eucaryotum are known to cause lysis of Gymnodinium breve, which is Florida's red tide organism. Two cytolytic agents were previously identified as methyl palmitate and methyl stearate. In this study, the critical micelle concentrations of these substances were determined by ultraviolet light and turbidimetric methods to be 3.5 +/- 0.3 ppm (methyl stearate) and 4.3 +/- 0.6 (methyl palmitate). There were no significant differences in results obtained using the two methods.

  13. Breve declaracion de las sentencias y vocablos obscuros que en el libro del Transito de la muerte se hallan

    OpenAIRE

    Venegas, Alejo

    2015-01-01

    416 f. ; 8º (15 cm.) A obra inclúe, con título propio: Breve declaración de las sentencias y vocablos obscuros que en el libro del Transito de la muerte se hallan A segunda data obtida do colofón Sign.: A-Z8, a-z8, 2A-2F8. Grav. xil. na port. Iniciais Reclamos Colofón Erros na numeración das f. En esta vltima impression enmendada de muchos errores q[e] en las impressiones passadas tenia

  14. Inferencias sobre Grafos

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    Sira M. Allende

    2002-05-01

    Full Text Available El estudio de un juego puede ser modelado asumiendo que solo algunas partidas son observadas. Entonces el árbol del juego debe ser estimado utilizando información muestral. Similarmente ocurre al obtener información sobre el comportamiento de las decisiones tomadas por individuos muestreados sobre un árbol de decisión teórico. Al considerar una medida de probabilidad que caracterice el comportamiento de redes aleatorias se puede obtener un estimado del árbol asociado. Este problema es el analizado en este trabajo. Algunos resultados experimentales obtenidos usando Recocido Simulado ilustran el procedimiento. Su papel en el estudio de modelos económicos y de mercadeo es discutido.

  15. Escrito sobre el cuerpo

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    Antonio Malalana Ureña

    2013-09-01

    Full Text Available El catálogo, en una edición bilingüe español-inglés, es una pequeña guía de la exposición Shirin Neshat. Escrito sobre el cuerpo, muestra que se integra dentro de los actos de PHOTOESPAÑA13, el festival internacional de fotografía y artes visuales. El esquema del libro es sencillo y los textos son extremadamente divulgativos. El primero de ellos, Escrito sobre el cuerpo (pp. 10-15, cuyo autor es Octavio Zaya, comisario de la exposición, resume la trayectoria de esta intelectual, que "interactúa" con la fotografía y los formatos audiovisuales, y nos presenta la argumentación narrativa de los documentos expuestos, contextualizando la obra recopilada.

  16. Bifidobacterium breve B-3 exerts metabolic syndrome-suppressing effects in the liver of diet-induced obese mice: a DNA microarray analysis.

    Science.gov (United States)

    Kondo, S; Kamei, A; Xiao, J Z; Iwatsuki, K; Abe, K

    2013-09-01

    We previously reported that supplementation with Bifidobacterium breve B-3 reduced body weight gain and accumulation of visceral fat in a dose-dependent manner, and improved serum levels of total cholesterol, glucose and insulin in a mouse model of diet-induced obesity. In this study, we investigated the expression of genes in the liver using DNA microarray analysis and q-PCR to reveal the mechanism of these anti-obesity effects in this mouse model. Administration of B. breve B-3 led to regulated gene expression of pathways involved in lipid metabolism and response to stress. The results indicate that these regulations in the liver are related to the anti-metabolic syndrome effects of B. breve B-3.

  17. Functional genome analysis of Bifidobacterium breve UCC2003 reveals type IVb tight adherence (Tad) pili as an essential and conserved host-colonization factor

    Science.gov (United States)

    O'Connell Motherway, Mary; Zomer, Aldert; Leahy, Sinead C.; Reunanen, Justus; Bottacini, Francesca; Claesson, Marcus J.; O'Brien, Frances; Flynn, Kiera; Casey, Patrick G.; Moreno Munoz, Jose Antonio; Kearney, Breda; Houston, Aileen M.; O'Mahony, Caitlin; Higgins, Des G.; Shanahan, Fergus; Palva, Airi; de Vos, Willem M.; Fitzgerald, Gerald F.; Ventura, Marco; O'Toole, Paul W.; van Sinderen, Douwe

    2011-01-01

    Development of the human gut microbiota commences at birth, with bifidobacteria being among the first colonizers of the sterile newborn gastrointestinal tract. To date, the genetic basis of Bifidobacterium colonization and persistence remains poorly understood. Transcriptome analysis of the Bifidobacterium breve UCC2003 2.42-Mb genome in a murine colonization model revealed differential expression of a type IVb tight adherence (Tad) pilus-encoding gene cluster designated “tad2003.” Mutational analysis demonstrated that the tad2003 gene cluster is essential for efficient in vivo murine gut colonization, and immunogold transmission electron microscopy confirmed the presence of Tad pili at the poles of B. breve UCC2003 cells. Conservation of the Tad pilus-encoding locus among other B. breve strains and among sequenced Bifidobacterium genomes supports the notion of a ubiquitous pili-mediated host colonization and persistence mechanism for bifidobacteria. PMID:21690406

  18. Functional genome analysis of Bifidobacterium breve UCC2003 reveals type IVb tight adherence (Tad) pili as an essential and conserved host-colonization factor.

    Science.gov (United States)

    O'Connell Motherway, Mary; Zomer, Aldert; Leahy, Sinead C; Reunanen, Justus; Bottacini, Francesca; Claesson, Marcus J; O'Brien, Frances; Flynn, Kiera; Casey, Patrick G; Munoz, Jose Antonio Moreno; Kearney, Breda; Houston, Aileen M; O'Mahony, Caitlin; Higgins, Des G; Shanahan, Fergus; Palva, Airi; de Vos, Willem M; Fitzgerald, Gerald F; Ventura, Marco; O'Toole, Paul W; van Sinderen, Douwe

    2011-07-05

    Development of the human gut microbiota commences at birth, with bifidobacteria being among the first colonizers of the sterile newborn gastrointestinal tract. To date, the genetic basis of Bifidobacterium colonization and persistence remains poorly understood. Transcriptome analysis of the Bifidobacterium breve UCC2003 2.42-Mb genome in a murine colonization model revealed differential expression of a type IVb tight adherence (Tad) pilus-encoding gene cluster designated "tad(2003)." Mutational analysis demonstrated that the tad(2003) gene cluster is essential for efficient in vivo murine gut colonization, and immunogold transmission electron microscopy confirmed the presence of Tad pili at the poles of B. breve UCC2003 cells. Conservation of the Tad pilus-encoding locus among other B. breve strains and among sequenced Bifidobacterium genomes supports the notion of a ubiquitous pili-mediated host colonization and persistence mechanism for bifidobacteria.

  19. Breve historia de la ventilación mecánica

    OpenAIRE

    Salas Segura, Donato A.

    2000-01-01

    Se dan unos adarmes sobre la historia del desarrollo de la ventilación mecánica asistida, una de las tecnologías médicas que más ha revolucionado el cuidado del paciente gravemente enfermo y que frecuentemente es ignorada en los textos y publicaciones de la historia de la medicina. UCR::Docencia::Salud::Facultad de Medicina::Escuela de Tecnologías en Salud

  20. Glycoside hydrolase family 13 α-glucosidases encoded by Bifidobacterium breve UCC2003; A comparative analysis of function, structure and phylogeny.

    Science.gov (United States)

    Kelly, Emer D; Bottacini, Francesca; O'Callaghan, John; Motherway, Mary O'Connell; O'Connell, Kerry Joan; Stanton, Catherine; van Sinderen, Douwe

    2016-05-02

    Bifidobacterium breve is a noted inhabitant and one of the first colonizers of the human gastro intestinal tract (GIT). The ability of this bacterium to persist in the GIT is reflected by the abundance of carbohydrate-active enzymes that are encoded by its genome. One such family of enzymes is represented by the α-glucosidases, of which three, Agl1, Agl2 and MelD, have previously been identified and characterized in the prototype B. breve strain UCC2003. In this report, we describe an additional B. breve UCC2003-encoded α-glucosidase, along with a B. breve UCC2003-encoded α-glucosidase-like protein, designated here as Agl3 and Agl4, respectively, which together with the three previously described enzymes belong to glycoside hydrolase (GH) family 13. Agl3 was shown to exhibit hydrolytic specificity towards the α-(1→6) linkage present in palatinose; the α-(1→3) linkage present in turanose; the α-(1→4) linkages found in maltotriose and maltose; and to a lesser degree, the α-(1→2) linkage found in sucrose and kojibiose; and the α-(1→5) linkage found in leucrose. Surprisingly, based on the substrates analyzed, Agl4 did not exhibit biologically relevant α-glucosidic activity. With the presence of four functionally active GH13 α-glucosidases, B. breve UCC2003 is capable of hydrolyzing all α-glucosidic linkages that can be expected in glycan substrates in the lower GIT. This abundance of α-glucosidases provides B. breve UCC2003 with an adaptive ability and metabolic versatility befitting the transient nature of growth substrates in the GIT. Copyright © 2016 Elsevier B.V. All rights reserved.

  1. Gurruminos, petimetres, abates y currutacos en el teatro breve del siglo XVIII

    Directory of Open Access Journals (Sweden)

    Sala Valldaura, Josep Maria

    2009-12-01

    Full Text Available This study of certain negative models of sainetes [afterpieces] from the 18th century reveals not only a continuity of comic stereotypes, but also their relationship to the ideals of behaviour of the era: the authority of the husband, the submissiveness of the wife, thriftiness in managing household finances, etc. Afterpieces join other literary genres (journalism, satirical poetry and the moral essay in ridiculing those who are involved in the practice of cortejo and who readily adopt new forms of social behavior, fashions, foreign dances, etc., in contrast to traditional ways and tastes. Examples taken from Antonio de Zamora, Agramont, Ramón de la Cruz, Vázquez and González del Castillo are used to illustrate this reticence towards changes in mentality of a new culture of leisure, as well as to observe it a contrario: its “feminization”, its frivolity, and its uselessness.El estudio de ciertos modelos negativos del teatro breve del siglo XVIII revela no sólo la continuidad cómica de los tipos, sino también su relación con los ideales de conducta coetáneos: la autoridad del marido, la discreción de la mujer, el cuidado de la economía familiar... Los sainetes se unen a otros géneros literarios (el periodismo, la poesía satírica y el ensayo de carácter moral en la ridiculización de quienes practican el cortejo y aplauden nuevas formas de sociabilidad, las modas y los bailes extranjeros, etc. frente a la conducta y los gustos tradicionales. Ejemplos tomados de Antonio de Zamora, Agramont, Ramón de la Cruz, Vázquez y González del Castillo sirven para argumentar tales reticencias ante el cambio de mentalidades de una nueva cultura del ocio, así como para observarlo a contrario: su “afeminación”, su frivolidad y su inutilidad.

  2. Icebreakers, Fillers y Warmers: actividades breves para la clase de inglés

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    Ramiro DURÁN MARTÍNEZ

    2009-11-01

    Full Text Available RESUMEN: En el siguiente artículo vamos a presentar diversos tipos de ejercicios de carácter breve que hemos utilizado en la clase de inglés con el objetivo de facilitar a los alumnos la práctica de la destreza oral. Estas actividades tienen distintos nombres dependiendo de la función que desempeñen: icebreakers, fillers y warmers. Se denominan icebreakers los ejercicios diseñados para romper la tensión que normalmente rodea las primeras sesiones de cualquier nueva actividad, como, por ejemplo, la primera clase de un curso de inglés. Cuando se habla de fillers se enfatiza su función comodín: tareas independientes que normalmente sirven para completar los últimos minutos del horario establecido para la clase de idiomas. El término warmer se aplica a las actividades que se llevan a cabo después de un período vacacional con el propósito de favorecer el reencuentro del alumno con el idioma que está estudiando. El principal objetivo de estos ejercicios es el desarrollo de la capacidad de los alumnos para expresarse de forma oral utilizando la lengua inglesa, concentrándose más en la práctica de la fluidez (fluency que en la precisión (accuracy. Por otra parte, sirven para favorecer la creación de vínculos de unión entre un grupo de estudiantes.ABSTRACT: In this paper, we are going to present a number of short activities that have been used in the English class in order to give students extra speaking practice. These activities were given different names depending on the role they play in the class: icebreakers, fillers and warmers. Icebreakers are fluency practice exercises produced to defuse the tension that the first sessions of every new activity imply: i.e. the first lesson of English. When talking about fillers, we refer to short independent activities that are used when the projected exercises have taken less time than expected. Warmers are also fluency practice activities devised to put students back in touch with the

  3. Terapia Interpessoal: um modelo breve e focal Interpersonal Therapy: a brief and focal model

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    Marcelo Feijó de Mello

    2004-06-01

    Full Text Available A psicoterapia é uma forma particular de tratamento em psiquiatria. Seu uso é amplamente difundido e conta com várias linhas de abordagem. OBJETIVO: Neste trabalho, o autor faz considerações a respeito da terapia interpessoal (TIP, uma forma breve e focal de psicoterapia. Inicialmente usada para o tratamento da depressão, teve seu espectro de ação ampliado para outros transtornos. A TIP pode ser dividida em 3 fases: inicial, quando é feito um diagnóstico do transtorno nomeando os sintomas ao paciente e diagnosticando o foco interpessoal a ser trabalhado; fase intermediária, onde se trabalha o foco; e fase final, quando o paciente é encorajado a reconhecer e consolidar seus ganhos para usá-los no futuro. MÉTODOS: O autor faz uma revisão das evidências científicas da eficácia da TIP no tratamento da depressão. CONCLUSÕES: Os resultados mostram que a TIP é uma forma terapêutica eficaz e bem tolerada de tratamento para a depressão.Psychotherapy is a particular form of treatment in Psychiatry. Its use is widespread and has many different approaches. OBJECTIVE: In this article, the author makes some considerations about Interpersonal Therapy (IPT, a brief and focal psychotherapy. Initially created to treat depression, other researchers had successfully increase its spectrum. It could be divided in three phases. Initial, when the therapist makes the diagnosis of the disorder and also the interpersonal problematic is pointed out; the patient received a sick role. Intermediary when the focus is treated, and the final phase when the therapist encourages the patient to recognize and consolidate gains and prepare the patient to use it in the future. METHODS: It is stressed that IPT is a testable form of psychotherapy and the scientific evidences of its efficacy are showed. CONCLUSIONS: The results assure that IPT is an efficient form of psychotherapy for depression with a great acceptability from the patients.

  4. Effect of Bifidobacterium breve M-16V supplementation on fecal bifidobacteria in preterm neonates--a randomised double blind placebo controlled trial.

    Directory of Open Access Journals (Sweden)

    Sanjay Patole

    Full Text Available BACKGROUND: Probiotic supplementation significantly reduces the risk of necrotising enterocolitis (NEC and all cause mortality in preterm neonates. Independent quality assessment is important before introducing routine probiotic supplementation in this cohort. AIM: To assess product quality, and confirm that Bifidobacterium breve (B. breve M-16V supplementation will increase fecal B. breve counts without adverse effects. METHODS AND PARTICIPANTS: Strain identity (16S rRNA gene sequencing, viability over 2 year shelf-life were confirmed, and microbial contamination of the product was ruled out. In a controlled trial preterm neonates (Gestation <33 weeks ready to commence or on feeds for <12 hours were randomly allocated to either B. breve M-16V (3×109 cfu/day or placebo (dextrin supplementation until the corrected age 37 weeks. Stool samples were collected before (S1 and after 3 weeks of supplementation (S2 for studying fecal B. breve levels using quantitative PCR (Primary outcome. Secondary outcomes included total fecal bifidobacteria and NEC≥Stage II. Categorical and continuous outcomes were analysed using Chi-square and Mann-Whitney tests, and McNemar and Wilcoxon signed-rank tests for paired comparisons. RESULTS: A total of 159 neonates (Probiotic: 79, Placebo: 80 were enrolled. Maternal and neonatal demographic characteristics were comparable between the groups. The proportion of neonates with detectable B. breve increased significantly post intervention: Placebo: [S1:2/66 (3%, S2: 25/66 (38%, p<0.001] Probiotic: [S1: 29/74 (40%, S2: 67/74 (91%, p<0.001]. Median S1 B. breve counts in both groups were below detection (<4.7 log cells x g(-1, increasing significantly in S2 for the probiotic group (log 8.6 while remaining <4.7 log in the control group (p<0.001. There were no adverse effects including probiotic sepsis and no deaths. NEC≥Stage II occurred in only 1 neonate (placebo group. CONCLUSION: B. breve M-16V is a suitable probiotic

  5. Bifidobacterium breve with α-linolenic acid and linoleic acid alters fatty acid metabolism in the maternal separation model of irritable bowel syndrome.

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    Eoin Barrett

    Full Text Available The aim of this study was to compare the impact of dietary supplementation with a Bifidobacterium breve strain together with linoleic acid & α-linolenic acid, for 7 weeks, on colonic sensitivity and fatty acid metabolism in rats. Maternally separated and non-maternally separated Sprague Dawley rats (n = 15 were orally gavaged with either B. breve DPC6330 (10(9 microorganisms/day alone or in combination with 0.5% (w/w linoleic acid & 0.5% (w/w α-linolenic acid, daily for 7 weeks and compared with trehalose and bovine serum albumin. Tissue fatty acid composition was assessed by gas-liquid chromatography and visceral hypersensitivity was assessed by colorectal distension. Significant differences in the fatty acid profiles of the non-separated controls and maternally separated controls were observed for α-linolenic acid and arachidonic acid in the liver, oleic acid and eicosenoic acid (c11 in adipose tissue, and for palmitoleic acid and docosahexaenoic acid in serum (p<0.05. Administration of B. breve DPC6330 to MS rats significantly increased palmitoleic acid, arachidonic acid and docosahexaenoic acid in the liver, eicosenoic acid (c11 in adipose tissue and palmitoleic acid in the prefrontal cortex (p<0.05, whereas feeding B. breve DPC6330 to non separated rats significantly increased eicosapentaenoic acid and docosapentaenoic acid in serum (p<0.05 compared with the NS un-supplemented controls. Administration of B. breve DPC6330 in combination with linoleic acid and α-linolenic acid to maternally separated rats significantly increased docosapentaenoic acid in the serum (p<0.01 and α-linolenic acid in adipose tissue (p<0.001, whereas feeding B. breve DPC6330 with fatty acid supplementation to non-separated rats significantly increased liver and serum docosapentaenoic acid (p<0.05, and α-linolenic acid in adipose tissue (p<0.001. B. breve DPC6330 influenced host fatty acid metabolism. Administration of B. breve DPC6330 to maternally separated

  6. Effect of Bifidobacterium breve on the Intestinal Microbiota of Coeliac Children on a Gluten Free Diet: A Pilot Study

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    Andrea Quagliariello

    2016-10-01

    Full Text Available Coeliac disease (CD is associated with alterations of the intestinal microbiota. Although several Bifidobacterium strains showed anti-inflammatory activity and prevention of toxic gliadin peptides generation in vitro, few data are available on their efficacy when administered to CD subjects. This study evaluated the effect of administration for three months of a food supplement based on two Bifidobacterium breve strains (B632 and BR03 to restore the gut microbial balance in coeliac children on a gluten free diet (GFD. Microbial DNA was extracted from faeces of 40 coeliac children before and after probiotic or placebo administration and 16 healthy children (Control group. Sequencing of the amplified V3-V4 hypervariable region of 16S rRNA gene as well as qPCR of Bidobacterium spp., Lactobacillus spp., Bacteroides fragilis group Clostridium sensu stricto and enterobacteria were performed. The comparison between CD subjects and Control group revealed an alteration in the intestinal microbial composition of coeliacs mainly characterized by a reduction of the Firmicutes/Bacteroidetes ratio, of Actinobacteria and Euryarchaeota. Regarding the effects of the probiotic, an increase of Actinobacteria was found as well as a re-establishment of the physiological Firmicutes/Bacteroidetes ratio. Therefore, a three-month administration of B. breve strains helps in restoring the healthy percentage of main microbial components.

  7. The combination of Bifidobacterium breve with non-digestible oligosaccharides suppresses airway inflammation in a murine model for chronic asthma.

    Science.gov (United States)

    Sagar, Seil; Vos, Arjan P; Morgan, Mary E; Garssen, Johan; Georgiou, Niki A; Boon, Louis; Kraneveld, Aletta D; Folkerts, Gert

    2014-04-01

    Over the last decade, there has been a growing interest in the use of interventions that target the intestinal microbiota as a treatment approach for asthma. This study is aimed at exploring the therapeutic effects of long-term treatment with a combination of Bifidobacterium breve with non-digestible oligosaccharides on airway inflammation and remodeling. A murine ovalbumin-induced chronic asthma model was used. Pulmonary airway inflammation; mRNA expression of pattern recognition receptors, Th-specific cytokines and transcription factors in lung tissue; expression of Foxp3 in blood Th cells; in vitro T cell activation; mast cell degranulation; and airway remodeling were examined. The combination of B. breve with non-digestible oligosaccharides suppressed pulmonary airway inflammation; reduced T cell activation and mast cell degranulation; modulated expression of pattern recognition receptors, cytokines and transcription factors; and reduced airway remodeling. The treatment induced regulatory T cell responses, as shown by increased Il10 and Foxp3 transcription in lung tissue, and augmented Foxp3 protein expression in blood CD4+CD25+Foxp3+ T cells. This specific combination of beneficial bacteria with non-digestible oligosaccharides has strong anti-inflammatory properties, possibly via the induction of a regulatory T cell response, resulting in reduced airway remodeling and, therefore, may be beneficial in the treatment of chronic inflammation in allergic asthma. Copyright © 2014 Elsevier B.V. All rights reserved.

  8. A combination of scGOS/lcFOS with Bifidobacterium breve M-16V protects suckling rats from rotavirus gastroenteritis.

    Science.gov (United States)

    Rigo-Adrover, M; Saldaña-Ruíz, S; van Limpt, K; Knipping, K; Garssen, J; Knol, J; Franch, A; Castell, M; Pérez-Cano, F J

    2017-06-01

    Rotavirus (RV) is the leading cause of severe diarrhoea among infants and young children, and although more standardized studies are needed, there is evidence that probiotics can help to fight against RV and other infectious and intestinal pathologies. On the other hand, the effects of prebiotics have not been properly addressed in the context of an RV infection. The aim of this study was to demonstrate a protective role for a specific scGOS/lcFOS 9:1 prebiotic mixture (PRE) separately, the probiotic Bifidobacterium breve M-16V (PRO) separately and the combination of the prebiotic mixture and the probiotic (synbiotic, SYN) in a suckling rat RV infection model. The animals received the intervention from the 3rd to the 21st day of life by oral gavage. On day 7, RV was orally administered. Clinical parameters and immune response were evaluated. The intervention with the PRO reduced the incidence, severity and duration of the diarrhoea (p Bifidobacterium breve M-16V or a combination of both is highly effective in modulating RV-induced diarrhoea in this preclinical model.

  9. Effect of Bifidobacterium breve on the Intestinal Microbiota of Coeliac Children on a Gluten Free Diet: A Pilot Study.

    Science.gov (United States)

    Quagliariello, Andrea; Aloisio, Irene; Bozzi Cionci, Nicole; Luiselli, Donata; D'Auria, Giuseppe; Martinez-Priego, Llúcia; Pérez-Villarroya, David; Langerholc, Tomaž; Primec, Maša; Mičetić-Turk, Dušanka; Di Gioia, Diana

    2016-10-22

    Coeliac disease (CD) is associated with alterations of the intestinal microbiota. Although several Bifidobacterium strains showed anti-inflammatory activity and prevention of toxic gliadin peptides generation in vitro, few data are available on their efficacy when administered to CD subjects. This study evaluated the effect of administration for three months of a food supplement based on two Bifidobacterium breve strains (B632 and BR03) to restore the gut microbial balance in coeliac children on a gluten free diet (GFD). Microbial DNA was extracted from faeces of 40 coeliac children before and after probiotic or placebo administration and 16 healthy children (Control group). Sequencing of the amplified V3-V4 hypervariable region of 16S rRNA gene as well as qPCR of Bidobacterium spp., Lactobacillus spp., Bacteroides fragilis group Clostridium sensu stricto and enterobacteria were performed. The comparison between CD subjects and Control group revealed an alteration in the intestinal microbial composition of coeliacs mainly characterized by a reduction of the Firmicutes/Bacteroidetes ratio, of Actinobacteria and Euryarchaeota . Regarding the effects of the probiotic, an increase of Actinobacteria was found as well as a re-establishment of the physiological Firmicutes/Bacteroidetes ratio. Therefore, a three-month administration of B. breve strains helps in restoring the healthy percentage of main microbial components.

  10. The Modular Teaching Organization System (MTOS from the perspective of graduates in the municipality of Breves - Pará

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    João Marcelino Pantoja Rodrigues

    2018-04-01

    Full Text Available This work is the result of a research in education. It analyzes contributions and limitations of the Modular Teaching Organization System in the education of young people in the rural environment of the municipality of Breves, Pará, from the perceptions of high school graduates. It considers the needs and expectations of this public. The research was carried out through a qualitative field study in the Mainardi village, a pioneer community in the offering of this program in the rural environment of Breves. The main technique of data collection is a semi-structured interview with six young graduates, whose responses were treated in light of the thematic content analysis. The results point to the affirmation of the importance of this program as the only alternative of access to secondary education for a large portion of young people from the rural environment of Pará. At the same time, the results reveal the mismatch of the experience in relation to the needs and expectations of this public of secondary education.

  11. Mechanistic Study of Utilization of Water-Insoluble Saccharomyces cerevisiae Glucans by Bifidobacterium breve Strain JCM1192.

    Science.gov (United States)

    Keung, Hoi Yee; Li, Tsz Kai; Sham, Lok To; Cheung, Man Kit; Cheung, Peter Chi Keung; Kwan, Hoi Shan

    2017-04-01

    Bifidobacteria exert beneficial effects on hosts and are extensively used as probiotics. However, due to the genetic inaccessibility of these bacteria, little is known about their mechanisms of carbohydrate utilization and regulation. Bifidobacterium breve strain JCM1192 can grow on water-insoluble yeast ( Saccharomyces cerevisiae ) cell wall glucans (YCWG), which were recently considered as potential prebiotics. According to the results of 1 H nuclear magnetic resonance (NMR) spectrometry, the YCWG were composed of highly branched (1→3,1→6)-β-glucans and (1→4,1→6)-α-glucans. Although the YCWG were composed of 78.3% β-glucans and 21.7% α-glucans, only α-glucans were consumed by the B. breve strain. The ABC transporter ( malEFG1 ) and pullulanase ( aapA ) genes were transcriptionally upregulated in the metabolism of insoluble yeast glucans, suggesting their potential involvement in the process. A nonsense mutation identified in the gene encoding an ABC transporter ATP-binding protein (MalK) led to growth failure of an ethyl methanesulfonate-generated mutant with yeast glucans. Coculture of the wild-type strain and the mutant showed that this protein was responsible for the import of yeast glucans or their breakdown products, rather than the export of α-glucan-catabolizing enzymes. Further characterization of the carbohydrate utilization of the mutant and three of its revertants indicated that this mutation was pleiotropic: the mutant could not grow with maltose, glycogen, dextrin, raffinose, cellobiose, melibiose, or turanose. We propose that insoluble yeast α-glucans are hydrolyzed by extracellular pullulanase into maltose and/or maltooligosaccharides, which are then transported into the cell by the ABC transport system composed of MalEFG1 and MalK. The mechanism elucidated here will facilitate the development of B. breve and water-insoluble yeast glucans as novel synbiotics. IMPORTANCE In general, Bifidobacterium strains are genetically intractable

  12. A ideologia do desenvolvimento sustentável: um breve balanço sobre sua prática no ensino de geografia

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    Leandro Dias de Oliveira

    2014-01-01

    Full Text Available Este artigo, escrito no âmbito do Curso de Geografia da FERLAGOS (Faculdade da Região dos Lagos, tem como escopo realizar um balanço de dez anos – entre a publicação da monografia intitulada “A Ideologia do Desenvolvimento Sustentável no Ensino de Geografia”, de L. D. de Oliveira (2001 e as atuais experiências docentes e de pesquisa de seus autores – da adoção do Desenvolvimento Sustentável no ensino de Geografia nos níveis fundamental e médio.

  13. Brief notations on the electronic commerce Breves anotações sobre o comércio eletrônico

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    Sandra Barbon Lewis

    2005-12-01

    Full Text Available This study verifies the aspects of the business concentration in the electronic commerce. For that, primarily, an analysis of these companies’ role in the global context is made, starting from technological advances as well as from the new world situation that both presented to them. The instantaneous flow of information made possible by the Internet as well as the high level of competitiveness introduced by the world-wide order, both led the economy agents to seek inside the online business for a new perspective of both expanding to a new market and international business. After this first step, we move to identifying the formation, sprouting, development, and legal definition of the online business in order to understand its characteristics e modalities; to observe the structure of the online market and discuss the transition of a conventional economy model to a new electronic or digital model; to question the existence of an online or virtual company starting from the development of the Teoria da Empresa as well as its way the way of work of the company in the web. After this initial contingencies are covered, the aim of the present work was to focus on the business concentration which foments the corporation development, but also represents a problem of great economic repercussions. Being so, search was made throughout the legal literature, for the existent types of corporation concentration, and throughout Brazilian Antitrust Law, for the regulation on this concentration, especially, in the Article 54 of the 8,884/94 act which predicts the regulation on acts and contracts that may lead to a corporation concentration. After the presentation of the online transaction models made by the corporations, it was observed, in pratical cases, the potential business concentration as well as the difficulties of relating and applying the Brazilian Antitrust Law in these cases. Thus, before the endless resources made possible by the Internet and the new corporation ways of acting born with the online business, merges the necessity of identifying the aspects of the business concentration in this innovative online market.O estudo verifica aspectos da concentração empresarial no comércio eletrônico. Para tanto, primeiramente, é realizada uma análise do papel das empresas no contexto global a partir do avanço tecnológico que é posto à disposição e da nova ordem mundial que lhe é apresentada. O fluxo instantâneo de informações, possibilitado pelo avanço da Internet e o alto grau de competitividade introduzido pela Globalização, levaram os agentes econômicos a buscar no comércio pela Internet uma nova perspectiva de expansão a novos mercados e de comércio internacional. Em seguida passa-se a identificar a formação, surgimento, desenvolvimento e a definição jurídica do comércio eletrônico para trazer à tona suas características e modalidades; observar a estrutura do mercado eletrônico e discutir a passagem da economia convencional a uma nova economia, a economia eletrônica ou digital; questionar a existência de uma empresa eletrônica ou virtual a partir do desenvolvimento da Teoria da Empresa bem como seu modo de atuação na Grande rede. Suplantadas essas contingências iniciais o objetivo foi adentrar no âmbito da concentração empresarial que fomenta o desenvolvimento empresarial, mas também, representa um problema de grandes repercussões econômicas. Sendo assim, buscaram-se, na doutrina, as formas de concentração empresarial e, na Lei antitruste brasileira, a sua disciplina, especificamente o Art. 54 da Lei 8.884/94 que prevê o controle de atos e contratos que podem levar a concentração empresarial. Após a apresentação dos modelos de negócios realizados pelas empresas na Internet, casuisticamente, observou-se a potencialidade de concentração empresarial e as dificuldades de sua caracterização na aplicação da Lei antitruste. Assim, ante os infindáveis recursos possibilitados pela Internet e as novas práticas empresariais advindas da utilização do Comércio Eletrônico, surge a necessidade de identificar os aspectos da concentração comercial neste inovador mercado eletrônico.

  14. Formação de professores e ensino privado noturno: uma breve reflexão sobre cursos superiores de História

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    Rejane Penna

    2007-01-01

    Full Text Available Formation of professorsand nocturnal private education:one soon reflection on superiorcourses of History. In recent yearsit was attended, if not to a epistemológicalrevolution, some significanttransformations in the interpretationand writing of History.One searched to surpass a timewhere if it always worked the sametechniques of inquiry, also folloiedof the same sources and subjects.In the present text, from the concomitantexperience of working asa teacher in higher education andhistoriógrafa the Historical Archiveof Rio Grande do Sul, analyzesthe difficulties so that the processof historiographical renewal penetratesin the nocturnal private superioreducation of the courses ofHistory.

  15. Breves notas sobre a produção de espaço urbano de São Paulo e os movimentos de luta por transporte

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    Gustavo Mello

    2015-09-01

    Full Text Available Neste artigo, procuramos considerar alguns aspectos da influência da indústria de transporte, e particularmente da automobilística, na produção social do espaço urbano de São Paulo, tendo como ponto de partida a elaboração do Plano de Avenidas (1930 de Prestes Maia. A despeito da inexistência de um efetivo planejamento urbano, tais considerações objetivam delinear de modo bastante sumário e indicativo uma lógica subjacente ao crescimento paulistano, a qual possui nexos com a emergência de movimentos sociais e de processos organizativos em torno da pauta do transporte público, dimensão candente da chamada “questão urbana” brasileira. Com um escopo histórico mais reduzido, é a essa análise que dedicaremos a segunda parte do texto, salientando as particularidades de lutas por melhorias da mobilidade urbana que recentemente foram travadas, sob princípios e parâmetros pretensamente antagônicos em relação às formas tradicionais de organização de esquerda.

  16. Breves considerações sobre processo de transformação da existência dos pescadores artesanais na modernidade

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    Dauto João da Silveira

    2012-12-01

    Full Text Available Este artigo tem como objetivo discutir as transformações sociais sofridas pelos pescadores artesanais ao longo da história e o papel das forças produtivas na consolidação destes acontecimentos. Para alcançar estes objetivos usou-se o método materialista-dialético diante do qual as contradições da realidade social engendram elementos decisivos para a superação dos homens. Desse modo, percebeu-se que a modernidade foi responsável não só pela dissolução profunda das vida tradicional dos pescadores artesanais como também criou condições para que eles se apropriassem de novos arranjos materiais.

  17. Breves considerações sobre a evolução, impacto e profilaxia do parvovírus canino

    OpenAIRE

    Costa, M.; Nóbrega, C.; Mega, A.; Esteves, F.; Cruz, R.; Santos, C.; Vala, Helena; Santos, M.; Mesquita, J. R.

    2011-01-01

    A família Parvoviridae está dividida em duas subfamílias. A subfamília Parvovirinae, que inclui os vírus que afectam vertebrados e a subfamília Densovirinae, que contém os vírus que afectam insectos (MacLachlan & Dubovi, 2011). Dentro da subfamília Parvovirinae foram definidos cinco géneros: Erytrovírus, Dependovírus, Parvovírus, Amdovírus e Bocavírus.

  18. Efeitos de pesticidas sobre a fragilidade osmótica de eritrócitos – Uma breve revisão

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    Humberto Gabriel Rodrigues

    2009-08-01

    Full Text Available http://dx.doi.org/10.5007/2175-7925.2009v22n1p7 Apesar da sua aplicação em outros setores, a agricultura tem sido a maior fonte de contaminação ambiental por praguicidas. Alguns praguicidas e seus metabólitos têm sido encontrados como poluentes no fundo e superfície de águas, no solo, e na atmosfera e são prováveis responsáveis pela perda da biodiversidade e a deterioração de hábitats naturais. Dentre os herbicidas, o Roundup® (Brasil é o mais comum. Estudos recentes têm demonstrado que o glifosato, o pesticida mais utilizado na agricultura e considerado de baixo risco pelo EPA-US gera fragilidade osmótica de eritrócitos humanos e de ratos dentro das concentrações indicadas pelos fabricantes. As características da intoxicação por esse praguicida podem ser explicadas pela hipóxia causada pela hemólise dos eritrócitos. Os pesticidas podem estar associados com a aquisição de demências, com o aumento da população de idosos no país, o contato tempo-dependente dos indivíduos com substâncias nocivas aumentam e putativamente, o aumento de demências provocadas por fatores exógenos tende a aumentar também, desse modo a contaminação do ambiente por pesticidas e as doenças assim derivadas devem ser consideradas como um problema de saúde pública.

  19. O uso de antibióticos e as resistências bacterianas: breves notas sobre a sua evolução

    OpenAIRE

    Loureiro, Rui João; Roque, Fátima; Teixeira Rodrigues, António; Herdeiro, Maria Teresa; Ramalheira, Elmano

    2016-01-01

    A resistência bacteriana aos antibióticos é atualmente um dos problemas de saúde pública mais relevantes a nível global, dado que apresenta consequências clínicas e económicas preocupantes, estando associada ao uso inadequado de antibióticos. Portugal é, no contexto europeu, um país com um elevado consumo de antibióticos, apesar de uma diminuic¸ão no consumo destes fármacos nos últimos anos. A resistência bacteriana tem crescido acentuadamente, sendo que as bactérias Gram-posit...

  20. Reflexiones sobre el estado actual de la Criminología: Una breve referencia/Reflextions about the current state of criminology: a brief reference

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    Chris Eskridge

    2018-01-01

    Full Text Available La delincuencia y la desviación son materias importantes de estudio, y se debe tener cuidado de no divorciarlas de los eventos sociales y políticos contemporáneos. Una preocupación global obvia del crimen y de la justicia en el presente, es el ya muy discutido choque de civilizaciones. La Criminología juega un rol que tomar en este y otros desafíos contemporáneos, y ese es el propósito de esta presentación, el explorar el impacto potencial de la Criminología Académica y de la Educación en justicia en una paz social, justicia y equidad, y proponer un modelo para su futuro crecimiento y desarrollo.

  1. REFLEXÕES SOBRE A AVALIAÇÃO DE DESEMPENHO: UMA BREVE ANÁLISE DO SISTEMA TRADICIONAL E DAS NOVAS PROPOSTAS

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    Alexandre Shigunov Neto

    2002-05-01

    Full Text Available RESUMO
    O presente trabalho teve como objetivo realizar uma análise crítica do sistema tradicional de avaliação de desempenho e das novas propostas de avaliação de desempenho. A avaliação de desempenho acompanhou a evolução das organizações e sempre esteve de alguma forma refletindo as transformações sociais. Assim, este trabalho segmentou em três fases históricas o desenvolvimento do processo de avaliação de desempenho: sistema tradicional, sistema contemporâneo e propostas para o futuro. As mudanças sociais, culturais, políticas, econômicas e tecnológicas que estão ocorrendo em grande velocidade acarretam grandes transformações organizacionais, no mercado e na crescente competitividade. Nesse sentido, as organizações necessitam descobrir e desenvolver procedimentos e maneiras que envolvam e comprometam os funcionários com seus objetivos e minimizem os equívocos e disfunções dos tradicionais métodos de avaliação. As novas propostas de avaliação de desempenho buscam incorporar conceitos modernos de gestão de recursos humanos, liderança, qualidade, valorização e crescimento do ser humano e sistemas adequados de recompensa, o que vem exigindo maior responsabilidade e preparo dos quadros avaliadores.
    Palavras-chave: funcionário, desempenho, transformações.

  2. Los fines de la estética y de la política. Breves reflexiones sobre los fantasmas del arte contemporáneo

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    Esteban Marcos Dipaola

    2015-03-01

    Full Text Available El artículo indaga en problemas del arte contemporáneo para planearse las condiciones de la persistencia de la noción de un fin del arte en el campo de las estéticas contemporáneas. A partir de un seguimiento de perspectivas se propone la hipótesis que define a la estética actual como inmanente a sus condiciones políticas y sociales de expresión. Con ello se cuestiona la moderna idea de Representación y se introduce una manera de pensar las problemáticas estéticas contemporáneas como expresión y permanente movilidad de la experiencia vital de los tiempos presentes.

  3. Brief notations on the electronic commerce
    Breves anotações sobre o comércio eletrônico

    OpenAIRE

    Sandra Barbon Lewis; Sérgio Eduardo Canella

    2005-01-01

    This study verifies the aspects of the business concentration in the electronic commerce. For that, primarily, an analysis of these companies’ role in the global context is made, starting from technological advances as well as from the new world situation that both presented to them. The instantaneous flow of information made possible by the Internet as well as the high level of competitiveness introduced by the world-wide order, both led the economy agents to seek inside the online business ...

  4. Breves considerações sobre a concepção do objeto de estudo da Psicologia para Wundt e para Brentano

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    Jan Luiz Leonardi

    2011-04-01

    Full Text Available O século XIX testemunhou diversas tentativas de constituição de uma Psicologia científica, com o aparecimento de diferentes sistemas psicológicos. Entre eles, destacam-se o de Wilhelm Wundt e o de Franz Brentano. Ambos buscam imputar cientificidade à Psicologia, mas discordam quanto ao ponto de partida para a elaboração de um projeto de Psicologia. Este artigo tem por objetivo introduzir a concepção do objeto de estudo da Psicologia conforme apresentada em suas obras centrais. Wundt defende que o objeto de estudo da Psicologia é a experiência consciente imediata, composta de elementos mentais básicos que podem ser divididos em sensações e sentimentos. Brentano postula que a Psicologia é a ciência dos fenômenos psíquicos, atos mentais que têm direção para objetos cuja existência depende de tais atos

  5. Breve acercamiento a la cuestión género en las políticas europeas sobre alcohol, tabaco y fármacos

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    Cristina Urios

    2012-12-01

    Full Text Available Public policies is the context of analysis chosen in this article. Specifically, the one refering to alcohol, tobacco and medicament. Public policies make an approach towards theoretical settings which raise political debates and practical porcesses from empirical analysis. The genre perspective-as social construction-points out the visibility of women, the equal participation in institutions, the care of language and transversality. Alcohol, tobacco and medicament are substances which have ,as a commom characteristic, the manipulation fron powerful and complex economical interests that can, and indeed, do influence both public policies and political profitability. The main point of the article is a warning about some of the consequences of "invisibility" for women. Este trabajo quiere introducir una reflexión en torno al contexto de análisis que se abre en torno al alcohol, el tabaco y los fármacos, el de las políticas públicas. Frente al ámbito de política criminal que ha de abordarse en el tratamiento de las drogas denominadas ilegales, las políticas públicas se abren a marcos teóricos que permiten una ordenación tipológica de las políticas públicas. Y, en este ámbito, intentaremos apuntar en relación con el tema estudiado cómo cabe un análisis empírico de las políticas públicas en tanto en cuanto permiten un primer proceso de definición de problemas y de acceso de cuestiones a la agenda pública de actuación; con posterioridad, un proceso de negociación y, en tercer término, la articulación de escenarios organizativos y de gestión. En este proceso, todos los actores políticos interactúan en función de intereses, ideas y valores ligados a distribuciones cambiantes de poder y con reglas más o menos formalizadas dentro de una clave institucional. DOWNLOAD THIS PAPER FROM SSRN: http://ssrn.com/abstract=2111915

  6. Breves consideraciones sobre la asistencia médica forzosa a los internos en los centros penitenciarios Some brief commentaries about enforced medical care for prison inmates

    Directory of Open Access Journals (Sweden)

    J.E. Arribas López

    2009-10-01

    Full Text Available En una sentencia de octubre de 2005 la Audiencia Nacional, declara la responsabilidad patrimonial del Ministerio del Interior (Dirección General de Instituciones Penitenciarias, por apreciar una concurrencia de culpas entre un interno fallecido en prisión y la Administración, ya que, aunque observa que fue correcta la actuación de los servicios médicos penitenciarios en el reconocimiento, diagnóstico y seguimiento de las enfermedades que padecía, antes y durante su internamiento, sin embargo tuvieron que adoptar, ante las reiteradas negativas de aquél a someterse a la medicación prescrita, las medidas reglamentarias oportunas con la correspondiente autorización de la dirección del centro penitenciario, a fin de que se cumpliera el tratamiento indicado. Sentada la indiscutible responsabilidad de la Administración de actuar, velando por la vida, integridad y salud de los internos cuando se observa un riesgo directo e inmediato utilizando la fuerza si es preciso, cuando se trata de procesos patológicos de curso prolongado, que puede llevar a una limitación de los derechos del interno más allá de lo necesario y someterlo a la aplicación habitual de unas medidas de fuerza que están concebidas y reguladas sólo para situaciones excepcionales.A decision by the National Court in October 2005 declared that the Ministry of the Interior (General Directorate of Prisons held pecuniary liability as a result of a concurrence of guilt between a deceased prison inmate and the prison Administration. It was observed that the prison health care staff had acted correctly when checking, diagnosing and monitoring the illness of the patient before and during admission. However, when faced with repeated refusals by the inmate to accept the prescribed treatment, they had to take suitable regulatory measures to comply with the terms of the indicated treatment with authorisation from the prison director. The Administration has a clearly established and undeniable liability to care for the life, integrity and health of prison inmates when a direct and immediate risk is observed, using force if necessary to deal with prolonged pathological conditions. This may bring about restrictions of the inmate's rights beyond those considered to be strictly necessary and lead to him/her being subject to the use of enforcement measures that are conceived of and designed for purely exceptional circumstances.

  7. Um breve percurso histórico e cultural do alimento: Questionamentos sobre consumo alimentar, cultura e educação

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    Terezinha Camargo Pompeo Vinha

    2017-05-01

    Full Text Available The text that is presented offers a feast of ideas that are open to reflection on food, eating habits and their historically situated uses. The objective of this study is to indicate and interpret the discursive language of the possible meanings of food and its multiple affections in human consumption that crosses the interfaces between the dimensions of education and health. This writing goes through the methodological path of historiographical and philosophical research based on the reference to researchers of the theme of food, nourishment and eating habits, understanding that these habits are located in the field of culture and are not universal, inserting in the history as a cultural object. The results point to the importance of reflections and critical discussions, making it possible to understand the theme not only as an essential element for human subsistence, but also as a consumer product created according to the productive models available as an element carrier of ideologies and different readings in time and space. In this study we intend to substantiate knowledge about the theme of food and nutrition, with new possibilities for reflection. O texto que se apresenta oferece um banquete de ideias que se dispõem à reflexão a respeito do alimento, dos hábitos alimentares e de seus usos historicamente situados. O objetivo deste estudo é indicar e interpretar a linguagem discursiva depreendida dos possíveis significados do alimento e suas múltiplas afetações no consumo humano que perpassa as interfaces entre as dimensões da educação e da saúde. Nossa escrita percorre o caminho metodológico da investigação historiográfica e filosófica com base na referência a pesquisadores do tema do alimento, da alimentação e dos hábitos alimentares, entendendo que estes hábitos se situam no campo da cultura e não são universais, inserindo-se na história como objeto cultural. Os resultados encontrados apontam para a importância de reflexões e de discussões críticas, possibilitando o entendimento do tema tratado não somente como elemento imprescindível para a subsistência humana, mas também como produto de consumo criado de acordo com os modelos produtivos de que se dispõe, como elemento portador de ideologias e de diferentes leituras no tempo e no espaço. Pretendemos nesse estudo substantivar o conhecimento em torno do tema do alimento e da alimentação com novas possibilidades para reflexão.

  8. Breve nota sobre o II Colóquio Brasil-Japão (Tóquio, 29 de fevereiro - 7 de março de 1968

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    Maria Regina da Cunha Rodrigues

    1968-03-01

    Full Text Available Êsse Colóquio, continuação do I realizado em São Paulo de 25 a 27 de julho de 1966, cujos Anais foram objeto de recente publicação (*, ao lado do saldo positivo que conclaves dêsse tipo alcançam, apresentou algumas falhas que, com a devida vênia, mencionaremos a seguir.

  9. Terraplenes sobre suelos colapsables

    OpenAIRE

    Arnedo Gaute, Diego

    2004-01-01

    Esta tesina está motivada por el diseño y construcción del Canal Segarra-Garrigues. El trazado del canal atraviesa varios pequeños valles de fondo llano mediante terraplenes sobre los cuales circula el canal. En estas vaguadas se encuentran suelos limo-arcillosos de baja densidad cuya potencia puede llegar a ser de varios metros. Estos materiales, debido a su estructura abierta, son potencialmente colapsables una vez cargados si experimentan incrementos de humedad. Las deformacion...

  10. DISCUTINDO SOBRE SEXTING

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    Suzana da Conceição de Barros

    2013-07-01

    Full Text Available Na contemporaneidade, os sujeitos vêm se utilizando das diversas tecnologias digitais, para exporem e compartilharem sua intimidade, com intuito de seduzirem e tornarem-se visíveis. Esse fenômeno vem sendo chamado de sexting, e vem sendo aderido por sujeitos de várias faixas etárias. Assim, leitor/a, convido você a pensar sobre o sexting e o que a escola tem a ver com essa prática?

  11. Excertos sobre o sedentarismo

    OpenAIRE

    Palma,Alexandre; Vilaça,Murilo Mariano; Assis,Monique Ribeiro de

    2014-01-01

    O sedentarismo tem sido tratado como algo perfeitamente determinável, objetivo e, portanto, que pode ser apreendido. Por outro lado, o conceito de sedentarismo ainda permanece questionável e, por conseguinte, os instrumentos mostram-se inconsistentes. Neste sentido, o objetivo do presente estudo foi mostrar que diferentes discursos de verdade vêm concorrendo por esse conceito, o que nos motiva a pensar qual é a verdade ou, até mesmo, se há alguma verdade sobre ele. Após apresentarmos diferent...

  12. Sobre historia mundial hoy

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    Vera Weiler

    2001-01-01

    Full Text Available Los procesos de globalización son una realidad; su enorme impacto sobre la vida humana los ha convertido en un tema recurrente sobre el que se escribe y se comenta a diario también en Colombia. Los libros que de ellos tratan, ante todo los que 10 hacen en tono crítico, baten records en la industria editorial en todo el mundo. Las expectativas que acerca del futuro abrigan cientos de millones de personas se relacionan con 10 que ellas esperan, para bien o para mal, de la globalización. Las posturas que los gobernantes ocupan al respecto en el mundo son, cuando menos de aceptación, generalmente de activa participación en pos de la globalización. Por un lado, crece la preocupación y se multiplican las protestas; por el otro, dominan los razonamientos de los especialistas en materia de maximización de los rendimientos de los capitales que presentan la globalización, al estilo que se viene imponiendo, como el camino ineludible del gobierno universal de la eficiencia. ¿Tienen que decir algo los historiadores frente a todo esto?.

  13. Analysis of infant isolates of Bifidobacterium breve by comparative genome hybridization indicates the existence of new subspecies with marked infant specificity

    NARCIS (Netherlands)

    Boesten, R.; Schuren, F.; Wind, R.D.; Knol, J.; Vos, W.M. de

    2011-01-01

    A total of 20 Bifidobacterium strains were isolated from fecal samples of 4 breast- and bottle-fed infants and all were characterized as Bifidobacterium breve based on 16S rRNA gene sequence and metabolic analysis. These isolates were further characterized and compared to the type strains of B.

  14. Hipnoterapia y terapia breve centrada en soluciones aplicada a síntomas por abuso sexual infantil: Un estudio de caso

    OpenAIRE

    Sánchez, Teresa; Téllez, Arnoldo

    2016-01-01

    En el estudio de caso que se presenta se aplicaron estrategias de hipnosis clínica en un contexto de intervención de terapia breve centrada en soluciones a un paciente de 40 años, víctima de abuso sexual infantil. El paciente presentaba cambios en s

  15. Production of a conjugated fatty acid by Bifidobacterium breve LMC520 from α-linolenic acid: conjugated linolenic acid (CLnA).

    Science.gov (United States)

    Park, Hui Gyu; Cho, Hyung Taek; Song, Myoung-Chong; Kim, Sang Bum; Kwon, Eung Gi; Choi, Nag Jin; Kim, Young Jun

    2012-03-28

    This study was performed to characterize natural CLnA isomer production by Bifidobacterium breve LMC520 of human origin in comparison to conjugated linoleic acid (CLA) production. B. breve LMC520 was found to be highly active in terms of CLnA production, of which the major portion was identified as cis-9,trans-11,cis-15 CLnA isomer by GC-MS and NMR analysis. B. breve LMC520 was incubated for 48 h using MRS medium (containing 0.05% L-cysteine · HCl) under different environmental conditions such as atmosphere, pH, and substrate concentration. The high conversion rate of α-linolenic acid (α-LNA) to CLnA (99%) was retained up to 2 mM α-LNA, and the production was proportionally increased nearly 7-fold with 8 mM by the 6 h of incubation under anaerobic conditions at a wide range of pH values (between 5 and 9). When α-LNA was compared with linoleic acid (LA) as a substrate for isomerization by B. breve LMC520, the conversion of α-LNA was higher than that of LA. These results demonstrated that specific CLnA isomer could be produced through active bacterial conversion at an optimized condition. Because many conjugated octadecatrienoic acids in nature are shown to play many positive roles, the noble isomer found in this study has potential as a functional source.

  16. Breve historia del cine en el África negra. Cuando el cinc era "africano", pero sólo de nombre.

    OpenAIRE

    Speciale, Alessandra

    1999-01-01

    Speciale, A. (1999). Breve historia del cine en el África negra. Cuando el cinc era "africano", pero sólo de nombre. Nosferatu. Revista de cine. (30):4-19. http://hdl.handle.net/10251/41132. 4 19 30

  17. El Sistema de Registro de Información sobre Drogas: Un auxiliar diagnóstico en la evaluación de la farmacodependencia

    Directory of Open Access Journals (Sweden)

    GALVÁN- REYES JORGE

    1997-01-01

    Full Text Available El presente trabajo tiene como objetivo dar a conocer la utilidad del Sistema de Registro de Información sobre Drogas como fuente diagnóstica importante para la evaluación del fenómeno de la farmacodependencia en la Ciudad de México. Incluye una breve descripción del soporte metodológico de este sistema, se presentan de manera sintetizada algunos de sus resultados y se discute la importancia de sus datos como material de apoyo para el establecimiento de políticas en materia de investigación y prevención del problema. Se comentan las ventajas de contar con un sistema de este tipo sobre todo porque en nuestro país no existe un sistema de notificación obligatoria de uso de drogas.

  18. Evidencias Psicométricas de una Medida Breve de Resiliencia en Adultos Mayores Peruanos no Institucionalizados

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    Tomás Caycho-Rodríguez

    2018-04-01

    Full Text Available La resiliencia es comprendida como el dominio de recursos personales y factores contextuales que permiten un afrontamiento exitoso y el logro de una adaptación positiva ante los diferentes estresores que aparecen a lo largo de la vida, siendo así importante dentro del proceso de envejecimiento saludable y exitoso. En la actualidad se han desarrollado instrumentos breves para la medición de la resiliencia como la escala breve de resiliencia (BRCS - Brief Resilient Coping Scale que evalúa la capacidad de los individuos para hacer frente al estrés de manera adaptativa. En este sentido, el estudio ofrece evidencia de validez y fiabilidad de la BRCS en adultos mayores no institucionalizados peruanos. Se contó con la participación de 236 adultos mayores con una edad promedio de 72.8 años (DT = 6.90 de los cuales el 78.4% eran mujeres y el 21.6% hombres, quienes respondieron la versión en español del BRCS y otras escalas para medir la satisfacción con la vida, humor como afrontamiento y depresión. Mediante el análisis factorial confirmatorio se corrobora la estructura unidimensional de la BRCS. Los coeficientes de consistencia interna alfa de Cronbach y omega indicaron una adecuada fiabilidad de la BRCS. Tanto los índices de ajuste del modelo como los valores de los coeficientes de fiabilidad fueron mejores en comparación con los reportados en la literatura. La BRCS mostró una correlación positiva significativa con la satisfacción con la vida y el humor como afrontamiento (p < .01. Asimismo, se observó una correlación negativa significativa con depresión (p < .01. Los resultados muestran que la BRCS cuenta con evidencias de validez y fiabilidad que avala su empleo como medida breve de la resiliencia en adultos mayores peruanos.

  19. Cell-free culture supernatant of Bifidobacterium breve CNCM I-4035 decreases pro-inflammatory cytokines in human dendritic cells challenged with Salmonella typhi through TLR activation.

    Science.gov (United States)

    Bermudez-Brito, Miriam; Muñoz-Quezada, Sergio; Gomez-Llorente, Carolina; Matencio, Esther; Bernal, Maria J; Romero, Fernando; Gil, Angel

    2013-01-01

    Dendritic cells (DCs) constitute the first point of contact between gut commensals and our immune system. Despite growing evidence of the immunomodulatory effects of probiotics, the interactions between the cells of the intestinal immune system and bacteria remain largely unknown. Indeed,, the aim of this work was to determine whether the probiotic Bifidobacterium breve CNCM I-4035 and its cell-free culture supernatant (CFS) have immunomodulatory effects in human intestinal-like dendritic cells (DCs) and how they respond to the pathogenic bacterium Salmonella enterica serovar Typhi, and also to elucidate the molecular mechanisms involved in these interactions. Human DCs were directly challenged with B. breve/CFS, S. typhi or a combination of these stimuli for 4 h. The expression pattern of genes involved in Toll-like receptor (TLR) signaling pathway and cytokine secretion was analyzed. CFS decreased pro-inflammatory cytokines and chemokines in human intestinal DCs challenged with S. typhi. In contrast, the B. breve CNCM I-4035 probiotic strain was a potent inducer of the pro-inflammatory cytokines and chemokines tested, i.e., TNF-α, IL-8 and RANTES, as well as anti-inflammatory cytokines including IL-10. CFS restored TGF-β levels in the presence of Salmonella. Live B.breve and its supernatant enhanced innate immune responses by the activation of TLR signaling pathway. These treatments upregulated TLR9 gene transcription. In addition, CFS was a more potent inducer of TLR9 expression than the probiotic bacteria in the presence of S. typhi. Expression levels of CASP8 and IRAK4 were also increased by CFS, and both treatments induced TOLLIP gene expression. Our results indicate that the probiotic strain B. breve CNCM I-4035 affects the intestinal immune response, whereas its supernatant exerts anti-inflammatory effects mediated by DCs. This supernatant may protect immune system from highly infectious agents such as Salmonella typhi and can down-regulate pro

  20. Effects of Lactobacillus paracasei CNCM I-4034, Bifidobacterium breve CNCM I-4035 and Lactobacillus rhamnosus CNCM I-4036 on hepatic steatosis in Zucker rats.

    Science.gov (United States)

    Plaza-Diaz, Julio; Gomez-Llorente, Carolina; Abadia-Molina, Francisco; Saez-Lara, Maria Jose; Campaña-Martin, Laura; Muñoz-Quezada, Sergio; Romero, Fernando; Gil, Angel; Fontana, Luis

    2014-01-01

    We have previously described the safety and immunomodulatory effects of Lactobacillus paracasei CNCM I-4034, Bifidobacterium breve CNCM I-4035 and Lactobacillus rhamnosus CNCM I-4036 in healthy volunteers. The scope of this work was to evaluate the effects of these probiotic strains on the hepatic steatosis of obese rats. We used the Zucker rat as a genetic model of obesity. Zucker-Lepr(fa/fa) rats received one of three probiotic strains, a mixture of L. paracasei CNCM I-4034 and B. breve CNCM I-4035, or a placebo for 30 days. An additional group of Zucker-lean+/fa rats received a placebo for 30 days. No alterations in intestinal histology, in the epithelial, lamina propria, muscular layers of the ileal or colonic mucosa, or the submucosae, were observed in any of the experimental groups. Triacylglycerol content decreased in the liver of Zucker-Lepr(fa/fa) rats that were fed L. rhamnosus, B. breve, or the mixture of B. breve and L. paracasei. Likewise, the area corresponding to neutral lipids was significantly smaller in the liver of all four groups of Zucker-Lepr(fa/fa) rats that received probiotics than in rats fed the placebo. Zucker-Lepr(fa/fa) rats exhibited significantly greater serum LPS levels than Zucker-lean+/fa rats upon administration of placebo for 30 days. In contrast, all four groups of obese Zucker-Lepr(fa/fa) rats that received LAB strains exhibited serum LPS concentrations similar to those of Zucker-lean+/fa rats. Serum TNF-α levels decreased in the Zucker-Lepr(fa/fa) rats that received B. breve, L. rhamnosus, or the mixture, whereas L. paracasei feeding decreased IL-6 levels in the serum of Zucker-Lepr(fa/fa) rats. In conclusion, the probiotic strains reduced hepatic steatosis in part by lowering serum LPS, and had an anti-inflammatory effect in obese Zucker rats.

  1. Effects of Lactobacillus paracasei CNCM I-4034, Bifidobacterium breve CNCM I-4035 and Lactobacillus rhamnosus CNCM I-4036 on hepatic steatosis in Zucker rats.

    Directory of Open Access Journals (Sweden)

    Julio Plaza-Diaz

    Full Text Available We have previously described the safety and immunomodulatory effects of Lactobacillus paracasei CNCM I-4034, Bifidobacterium breve CNCM I-4035 and Lactobacillus rhamnosus CNCM I-4036 in healthy volunteers. The scope of this work was to evaluate the effects of these probiotic strains on the hepatic steatosis of obese rats. We used the Zucker rat as a genetic model of obesity. Zucker-Lepr(fa/fa rats received one of three probiotic strains, a mixture of L. paracasei CNCM I-4034 and B. breve CNCM I-4035, or a placebo for 30 days. An additional group of Zucker-lean+/fa rats received a placebo for 30 days. No alterations in intestinal histology, in the epithelial, lamina propria, muscular layers of the ileal or colonic mucosa, or the submucosae, were observed in any of the experimental groups. Triacylglycerol content decreased in the liver of Zucker-Lepr(fa/fa rats that were fed L. rhamnosus, B. breve, or the mixture of B. breve and L. paracasei. Likewise, the area corresponding to neutral lipids was significantly smaller in the liver of all four groups of Zucker-Lepr(fa/fa rats that received probiotics than in rats fed the placebo. Zucker-Lepr(fa/fa rats exhibited significantly greater serum LPS levels than Zucker-lean+/fa rats upon administration of placebo for 30 days. In contrast, all four groups of obese Zucker-Lepr(fa/fa rats that received LAB strains exhibited serum LPS concentrations similar to those of Zucker-lean+/fa rats. Serum TNF-α levels decreased in the Zucker-Lepr(fa/fa rats that received B. breve, L. rhamnosus, or the mixture, whereas L. paracasei feeding decreased IL-6 levels in the serum of Zucker-Lepr(fa/fa rats. In conclusion, the probiotic strains reduced hepatic steatosis in part by lowering serum LPS, and had an anti-inflammatory effect in obese Zucker rats.

  2. Cell-free culture supernatant of Bifidobacterium breve CNCM I-4035 decreases pro-inflammatory cytokines in human dendritic cells challenged with Salmonella typhi through TLR activation.

    Directory of Open Access Journals (Sweden)

    Miriam Bermudez-Brito

    Full Text Available Dendritic cells (DCs constitute the first point of contact between gut commensals and our immune system. Despite growing evidence of the immunomodulatory effects of probiotics, the interactions between the cells of the intestinal immune system and bacteria remain largely unknown. Indeed,, the aim of this work was to determine whether the probiotic Bifidobacterium breve CNCM I-4035 and its cell-free culture supernatant (CFS have immunomodulatory effects in human intestinal-like dendritic cells (DCs and how they respond to the pathogenic bacterium Salmonella enterica serovar Typhi, and also to elucidate the molecular mechanisms involved in these interactions. Human DCs were directly challenged with B. breve/CFS, S. typhi or a combination of these stimuli for 4 h. The expression pattern of genes involved in Toll-like receptor (TLR signaling pathway and cytokine secretion was analyzed. CFS decreased pro-inflammatory cytokines and chemokines in human intestinal DCs challenged with S. typhi. In contrast, the B. breve CNCM I-4035 probiotic strain was a potent inducer of the pro-inflammatory cytokines and chemokines tested, i.e., TNF-α, IL-8 and RANTES, as well as anti-inflammatory cytokines including IL-10. CFS restored TGF-β levels in the presence of Salmonella. Live B.breve and its supernatant enhanced innate immune responses by the activation of TLR signaling pathway. These treatments upregulated TLR9 gene transcription. In addition, CFS was a more potent inducer of TLR9 expression than the probiotic bacteria in the presence of S. typhi. Expression levels of CASP8 and IRAK4 were also increased by CFS, and both treatments induced TOLLIP gene expression. Our results indicate that the probiotic strain B. breve CNCM I-4035 affects the intestinal immune response, whereas its supernatant exerts anti-inflammatory effects mediated by DCs. This supernatant may protect immune system from highly infectious agents such as Salmonella typhi and can down

  3. Prevalence of HCV infection and associated factors among illicit drug users in Breves, State of Pará, northern Brazil.

    Science.gov (United States)

    Pacheco, Suzy Danielly Barbosa; Silva-Oliveira, Gláucia Caroline; Maradei-Pereira, Luciana Maria Cunha; Crescente, José Ângelo Barletta; Lemos, José Alexandre Rodrigues de; Oliveira-Filho, Aldemir Branco de

    2014-01-01

    Illicit drug users (DUs) are vulnerable to hepatitis C virus (HCV) infection. The shared use of illicit drugs is the main method of HCV transmission. A cross-sectional study was conducted in Breves, in northern Brazil. We surveyed 187 DUs to determine the prevalence of and factors associated with HCV infection. The prevalence of anti-HCV antibodies was 36.9%, and the prevalence of hepatitis C virus-ribonucleic acid (HCV-RNA) was 31%. Hepatitis C virus infection was associated with tattoos, intravenous drug use, shared use of equipment for drug use, drug use for longer than 3 years, and daily drug use. Strategies for preventing and controlling HCV transmission should be implemented among DUs.

  4. Prevalence of HCV infection and associated factors among illicit drug users in Breves, State of Pará, northern Brazil

    Directory of Open Access Journals (Sweden)

    Suzy Danielly Barbosa Pacheco

    2014-06-01

    Full Text Available Introduction: Illicit drug users (DUs are vulnerable to hepatitis C virus (HCV infection. The shared use of illicit drugs is the main method of HCV transmission. Methods: A cross-sectional study was conducted in Breves, in northern Brazil. We surveyed 187 DUs to determine the prevalence of and factors associated with HCV infection. Results: The prevalence of anti-HCV antibodies was 36.9%, and the prevalence of hepatitis C virus-ribonucleic acid (HCV-RNA was 31%. Hepatitis C virus infection was associated with tattoos, intravenous drug use, shared use of equipment for drug use, drug use for longer than 3 years, and daily drug use. Conclusions: Strategies for preventing and controlling HCV transmission should be implemented among DUs.

  5. Breve panorama de la psicología educacional en el Perú

    Directory of Open Access Journals (Sweden)

    Aníbal Meza

    1990-06-01

    Full Text Available Se da cuenta de los orígenes y los desarrollos temprano y reciente de la Psicología Educacional en el Perú; luego se hace una presentación por áreas temáticas o de actividad: Educación Especial, Estimulación Temprana, Investigación Psicoeducacional en varias formas (psicométrica, básica con proyecciones educacionales y otros. Además se consignan los trabajos teóricos y las revisiones de la literatura, así como la producción tecnológica (textos y pruebas psicológicas. Finalmente se recogen algunos balances sobre aspectos de la Psicología Educacional, se ensaya una evaluación de conjunto y se delinean algunas perspectivas de la disciplina en el país.

  6. Versão Portuguesa da Escala Breve de Redes Sociais de Lubben (LSNS-6)

    OpenAIRE

    Ribeiro, Oscar; Teixeira, Laetitia; Duarte, Natália; Azevedo, Maria João; Araújo, Lia; Barbosa, Susana; Paúl, Constança

    2012-01-01

    A avaliação das redes sociais na investigação e prática gerontológica requer o uso de instrumentos válidos e eficazes que sejam simples, concisos e de fácil aplicação na população idosa. A Escala de Redes Sociais de Lubben (LSNS) é um dos instrumentos mais utilizados para avaliar a integração social e o risco de isolamento social em idosos residentes na comunidade. Este artigo debruça-se sobre a tradução e validação da versão abreviada da escala (LSNS-6) para o Português Europeu e expõe as su...

  7. Overexpression and characterization of a novel transgalactosylic and hydrolytic β-galactosidase from a human isolate Bifidobacterium breve B24.

    Science.gov (United States)

    Yi, Sung Hun; Alli, Inteaz; Park, Kwan Hwa; Lee, Byonghoon

    2011-10-01

    After the complete gene of a β-galactosidase from human isolate Bifidobacterium breve B24 was isolated by PCR and overexpressed in E. coli, the recombinant β-galactosidase was purified to homogeneity and characterized for the glycoside transferase (GT) and glycoside hydrolase (GH) activities on lactose. One complete ORF encoding 691 amino acids (2,076 bp) was the structural gene, LacA (galA) of the β-gal gene. The recombinant enzyme shown by activity staining and gel-filtration chromatography was composed of a homodimer of 75 kDa with a total molecular mass of 150 kDa. The K(m) value for lactose (95.58 mM) was 52.5-fold higher than the corresponding K(m) values for the synthetic substrate ONPG (1.82 mM). This enzyme with the optimum of pH 7.0 and 45°C could synthesize approximately 42.00% of GOS from 1M of lactose. About 97.00% of lactose in milk was also quickly hydrolyzed by this enzyme (50 units) at 45°C for 5h to produce 46.30% of glucose, 46.60% of galactose and 7.10% of GOS. The results suggest that this recombinant β-galactosidase derived from a human isolate B. breve B24 may be suitable for both the hydrolysis and synthesis of galacto-oligosaccharides (GOS) in milk and lactose processing. Copyright © 2011 Elsevier B.V. All rights reserved.

  8. The effect of Lactobacillus casei and Bifidobacterium breve on antibiotic-associated diarrhea treatment: randomized double-blind clinical trial

    Directory of Open Access Journals (Sweden)

    Daniela Nogueira Prado de Souza

    2012-02-01

    Full Text Available INTRODUCTION: Antibiotic-associated diarrhea (AAD is an important side effect of this specific class of drugs. The objective of this study was to investigate the effect of the use of probiotics in the treatment of AAD. METHODS: A group of hospitalized patients, who contracted diarrhea during or after 7 days of suspension of antimicrobial medication, was blindly randomized to receive a standardized diet associated with the use of the probiotics (Lactobacillus casei and Bifidobacterium breve or its corresponding placebo, three times a day. RESULTS: Seventy patients were studied. For the experimental (n=35 and control (n=35 groups, respectively, the average time of treatment was 5.06±2.18 and 5.49±3.17 days (p=0.95, and the average duration of diarrhea, among those who were healed, was 4.87±2.13 and 4.52±2.55 days (p=0.36. Four (11.4% patients who received probiotics and ten (28.6% who received the placebo were not cured (p=0.13, and relapse rates were similar between both groups. Seven patients from each group, in addition to diarrhea, presented cases of bloating and/or abdominal cramps and/or vomiting (p=1.00. CONCLUSIONS: In this light, it is concluded that L. casei associated with B. breve, in the administered dosage and frequency, has no effect on the antibiotic-associated diarrhea. Similar studies need to be conducted with higher doses of these or other probiotics.

  9. Development of a luciferase-based reporter system to monitor Bifidobacterium breve UCC2003 persistence in mice

    Directory of Open Access Journals (Sweden)

    Hill Colin

    2008-09-01

    Full Text Available Abstract Background Probiotics such as bifidobacteria have been shown to maintain a healthy intestinal microbial balance and help protect against infections. However, despite these benefits, bifidobacteria still remain poorly understood at the biochemical, physiological and especially the genetic level. Herein we describe, for the first time, the development of a non-invasive luciferase-based reporter system for real-time tracking of Bifidobacterium species in vivo. Results The reporter vector pLuxMC1 is based on the recently described theta-type plasmid pBC1 from B. catenatulatum 1 and the luxABCDE operon from pPL2lux 2. Derivatives of pLuxMC1, harbouring a bifidobacterial promoter (pLuxMC2 as well as a synthetically derived promoter (pLuxMC3 3 placed upstream of luxABCDE, were constructed and found to stably replicate in B. breve UCC2003. The subsequent analysis of these strains allowed us to assess the functionality of pLuxMC1 both in vitro and in vivo. Conclusion Our results demonstrate the potential of pLuxMC1 as a real-time, non-invasive reporter system for Bifidobacterium. It has also allowed us, for the first time, to track the colonisation potential and persistence of this probiotic species in real time. An interesting and significant outcome of the study is the identification of the caecum as a niche environment for B. breve UCC2003 within the mouse gastrointestinal tract (GI tract.

  10. The use of ozone in an artificial seawater environment and its ability to degrade Gymnodinium breve toxins

    Energy Technology Data Exchange (ETDEWEB)

    Schneider, K.R.

    1991-01-01

    The objectives of this research were to establish the practicality of currently used oxidant tests for ozone-treated artificial seawater and to determine the effectiveness of using ozone to reduce toxins associated with Gymnodinium breve, the red tide-causing dinoflagellate found in the Gulf of Mexico off the coast of Florida. In addition to its beneficial role, some emphasis was placed on ascertaining if any harmful by-products could be formed during the ozonation process. Three tests using amperometric titration, potassium iodide (KI) and N,N-diethyl-p-phenylene-diamine (DPD) were performed to determine their ability to detect ozone-produced oxidants in various solutions. These methods yielded different results when bromine and ammonia concentrations were varied in an artificial seawater (ASW) environment. The KI test yielded up to 100 percent higher estimates for each sample than did the amperometric and DPD tests. To test for the possible production of harmful by-products during the ozonation process, ASW samples were spiked with 1 gram of hesperetin. In experiments where the seawater mix was exposed to 27 ppm of ozone prior to the introduction of the organic precursor, small but measurable amounts of tribromomethane were detected via gas chromatography/mass spectroscopy. As the ozone dose was increased to 135 ppm, the recoverable levels of tribromomethane increased. When G. breve toxins were exposed to ozone treatment, samples displayed a three log reduction in the total amount of toxin recovered after ten minutes. Reduction in toxin levels directly correlated with reduction of toxicity as determined by a fish bioassay. It is significant to report that even after 10 minutes of ozonation, comparable to dose levels of that might be used in a commercial depuration facility, some toxins were still recoverable by HPLC analysis.

  11. William Marçais y su obra sobre el dialecto árabe hablado en Tremecén. Trascendencia para la historia de la dialectología de Argelia

    OpenAIRE

    Ikram Hadef, Sarra

    2017-01-01

    Breve presentación de la figura del arabista francés William Marçais y sus estudios sobre el dialecto árabe de Tremecén, estudiando el contenido de la obra Le dialecte arabe parlé à Tlemcen. Grammaire, textes et glossaire (1902) y su labor pionera en la constitución de una dialectología moderna argelina. Short introduction to the life of the French Orientalist William Marçais and his works about the Arabic dialect of the region of Tlemcen, focusing attention on the contents of Le dialecte ...

  12. Percepción de docentes directivos, profesores, alumnos y apoderados sobre la incidencia de la JECD en el contexto institucional estudio de casos en la región metropolitana de Chile

    OpenAIRE

    Rebolledo Zurita, María Magdalena

    2012-01-01

    A modo de introducción hacemos una breve reseña sobre la sociedad actual, la expansión de la educación en América Latina y las políticas que llevaron a Chile a participar en un proceso de reforma educativa, posteriormente presentamos capítulo a capítulo el trabajo realizado. La mundialización de la modernidad se refiere principalmente a ese proceso de alargamiento de los métodos de conexión entre diferentes contextos sociales o regionales que se convierten en una red a lo largo de toda la...

  13. Periodismo científico: reflexiones sobre la práctica en América Latina

    Directory of Open Access Journals (Sweden)

    Luisa Massarani

    2012-11-01

    Full Text Available Este artículo tiene como objetivo hacer algunas reflexiones sobre el periodismo científico en América Latina. Empezaremos con un breve rescate de un movimiento iniciado en los años 1960, que se diseminó y resultó en la creación, de forma concertada, en asociaciones de periodismo científico en algunos países de la región. En seguida, presentaremos los datos de una encuesta a periodistas que cubren temas de ciencia y tecnología acerca de sus percepciones respecto de la práctica y las perspectivas de la profesión. De acuerdo con los resultados, hay optimismo entre los profesionales en el campo, lo que sugiere que las semillas dejadas por el movimiento de los años 1960 tienen ecos importantes en el presente.

  14. Problemática sobre la disponibilidad de los Bienes Jurídicos Individuales y Responsabilidades Emergentes

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    Ignacio Curbelo Solari

    2013-12-01

    Full Text Available Sobre el concepto de Disponibilidad. Concepto de Bien Jurídico y sus funciones. El Consentimiento. Breve Historia. Bienes Jurídicos Colectivos e Individuales. Naturaleza Jurídica del Consentimiento. Posición de Jescheck, Roxin y Muñoz Conde. Posición de los principales autores nacionales: Prof. Gonzalo Fernández, Miguel Langón, Germán Aller, Pedro Montano. Los requisitos del consentimiento. En la doctrina extranjeraEn la doctrina uruguaya El consentimiento presunto. Requisitos del consentimiento presunto.Relevancia del consentimiento en el Derecho Positivo. Derecho extranjero Derecho Uruguayo. Diversas situaciones. El art. 44 del CP. La Vida. El consentimiento y la responsabilidad de los médicos. Las intervenciones transexuales. La Eutanasia La ley 18.473 de Voluntad anticipada. La auto puesta en peligro.

  15. El 40 aniversario del INECOL. Algunas reflexiones sobre una destacada institución científica mexicana

    OpenAIRE

    Zunino, Mario

    2016-01-01

    Resumen: Se presentan algunas reflexiones acerca del INECOL (Instituto de Ecología A.C., Xalapa, México) a cuarenta años de su fundación en la Ciudad de México, su sucesivo traslado a Xalapa y, algunos meses después de la publicación de un libro sobre su historia. Un breve sumario de algunos acontecimientos de especial importancia en la vida y el desarrollo del Instituto, está acompañado de una evaluación de la importancia internacional de las actividades de investigación y formación académic...

  16. Análisis teórico y experimental sobre seguridad en redes Wi-Fi

    OpenAIRE

    Romero Navarro, Fernando Pablo

    2014-01-01

    Este proyecto realiza un minucioso estudio sobre los mecanismos de seguridad que están disponibles para los dispositivos Wi-Fi, conforme a las especificaciones de la versión del estándar IEEE 802.11 publicada en el año 2007, incluyendo también una breve descripción de las debilidades y de los ataques más importantes que han sido descubiertos y que afectan a estos mecanismos de seguridad. En lo que respecta a la parte práctica, se documenta tanto el análisis como el diseño y se discusten cuest...

  17. Clave para diferenciar los géneros de Palmae en la Amazonía a partir del aparato vegetativo --- complementada por una breve descripción de los géneros, la distribución geográfica y ecológica, y los nombres vernáculos más comunes

    Directory of Open Access Journals (Sweden)

    1990-01-01

    Full Text Available CLE POUR DIFFERENCIER LES GENRES DE PALMAE EN AMAZONIE A PARTIR DE L’APPAREIL VEGETATIF COMPLETEE PAR UNE BREVE DESCRIPTION DES GENRES, LA DISTRIBUTION GEOGRAPHIQUE ET ECOLOGIQUE ET LES NOMS VERNACULAIRES LES PLUS COURANTS. Une clé de détermination des genres de palmiers de l’Amazonie est proposée. Elle n’utilise que des caractères végétatifs et s’applique aux plantules, juvéniles et adultes. 41 genres de palmiers sont traités. Une description sommaire de chaque genre est donnée avec des informations sur sa distribution géographique, son écologie, ses noms vernaculaires les plus communs. Se propone una clave analítica para diferenciar a partir de los caracteres vegetativos los géneros de palmeras de la cuenca amazónica. Esta clave se aplica tanto a las plántulas y juveniles como a los adultos. Se provee también una breve descripción de los 41 géneros considerados, con datos sobre la distribución geográfica, ecología, y nombres vernáculos. KEY FOR IDENTIFICATION OF AMAZONIAN PALM GENERA FROM VEGETATIVE CHARACTERS WITH A SUPPLEMENT INCLUDING SHORT DESCRIPTIONS OF GENERA, NOTES ON DISTRIBUTION AREAS AND ECOLOGY, AND THE MOST COMMON VERNACULAR NAMES. A key for identification of Amazonian palm genera from vegetative characters is proposed. It is applied to seedlings, juvenile and adult palms. 41 genera are treated. A short description of each genus with data on distribution, ecology, and vernacular names is given.

  18. Analysing Old Testament poetry: Basic issues in contemporary exegesis

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    G. T. M. Prinsloo

    1991-08-01

    Full Text Available The wealth of publications on matters relating to Old Testament poetry is witness to the fact that this subject has become a focal point in Old Testament studies. In this paper, an overview of contemporary publications is given. The basic issues, both on the level of poetic theory and practical application, are pointed out. A tendency towards a comprehensive literary approach is definitely present and should be encouraged. Only when a poem is analysed on all levels and by all means, will the richness of its meaning be appreciated.

  19. the word that moves 1. academic exegesis and spiritual reading

    African Journals Online (AJOL)

    that a good teacher has to offer are earned through assiduous effort and hard work. ..... could she have pressed her petition, had she not first been fired by .... search for liturgical material against the background of the reading of. Scripture, the ...

  20. A brief exegesis of End Triassic Extinction issues

    Science.gov (United States)

    Whiteside, J. H.; Grice, K.; Fox, C.; Kent, D. V.; Olsen, P. E.; Irmis, R. B.

    2017-12-01

    Recent reports of environmental proxy records through the end-Triassic extinction (ETE), in some cases coupled with high-resolution geochronologic data, provide new insights into cause and effect. For example, the emplacement of vast volumes of basalt in the Central Atlantic Magmatic Province (CAMP) are temporally associated with carbon isotopic excursions (CIEs), indications of widespread oceanic euxinia, distinct regional and perhaps very abrupt global sea level change, massive changes in atmospheric CO2, and the proliferation of "disaster" species, both on land and ocean. In the least, these indicate major disruptions in how the Earth works. However some striking and critical issues remain unresolved at a very basic level. Most important are the uncertainties in the stratigraphic relationships of marine extinctions to the various environmental proxy sections, particularly the GSSP for the base Hettangian in Austria, and the UK sections (notably St. Audrie's Bay). Here, the sequence of sporomorph and marine "invertebrate" turnover occurs in different order relative to the proxy record and lithostratigraphy. Thus the sequence of environmental events are, at present, of uncertain relationship to the extinction. Second, it is unclear what processes the various CIEs reflect in different environments; the canonical initial isotopic excursion in the UK, demonstrably correlatable over a huge area, was recorded in a lake in a restricted basin, unlike the isotopic data from surrounding marine strata. Could some CIEs in non-marine basins be diagenetic in nature, caused by the contact effects of overlying basalts? Finally, how does the clear and dramatic tropical non-marine record of the ETE, precisely located relative to the CAMP, relate to the marine record of the ETE, particularly at higher latitudes, where continental biotic turnover is not nearly as dramatic? Do these records correlate in a sufficiently tight temporal interval such that causation can be inferred? These issues can be resolved by: 1) finding biologically-independent proxies of time, such as the magnetic polarity chron E23r in the marine and high latitude realms; and 2) appropriately designed resampling efforts in the marine sections to determine the position and magnitude of the ETE.

  1. Alman Reformistlerin Tefecilik Yorumu (Usury Exegesis of German Reformists

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    Kürşat Haldun AKALIN

    2010-01-01

    Full Text Available The German Reformation was caused to the outbreak of the modern revolt against the Hebraic and medieval catholician prohibition of usury. Because Luther asserted that the human was free, under no obligation to observe dead Mosaic ordinances. He explained that the Deuteronomic commantment on usury was not intented to take place of the civil law or to supplant existing secular authorities. The age of the German reformers is the time of Deuteronomy’s crisis and demise. Luther applauded the Deuteronomic discrimination. He insisted that God allowed the Hebrews to take usury from alien peoples not because usury was meritorious or a part of the common law, but because He wished thus to indicate His wrath against the Gentiles.Melanchton’s views on the traffic in annuities are much more than those of Luther. Melanchton’s formula implies the increasing bifurcation of human relations into distinct spheres: one, the world of friendship and free services; the other, the world of commercial intercourse and economic calculus. Zwingli (1484-1531 , like Luther and Melanchthon, seems loathe to concede that a strict prohibition of usury might be inferred from Scriptures. But in spite of this they don’t expressly depart from the ethic of brotherhood. Martin Bucer (1491-1551 says persons may rightly claim ten per cent interest under the law. According to him, the objections to usury depend on the Old Testament apply not to all increments on loans, but properly to bitting usury upon usury.

  2. Puente sobre el Maracaibo

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    Equipo Editorial

    2017-05-01

    Full Text Available Para salvar el lago de Maracaibo (Venezuela, el conocido ingeniero italiano Riccaido Morandi ha presentado un proyecto de puente para ferrocarril y carretera, de hormigón pretensado, de tramos independientes, constituyendo un sistema Gerber, que tiene una longitud total de 8.720 m y un vano central de 400 m de luz. Este último tramo, en unión de los dos adyacentes al mismo, constituirán, una vez terminada la obra, un conjunto continuo. El tablero del puente se halla a una altura de 45 m sobre el nivel del agua en la zona del canal, con objeto de permitir la navegación. El tablero, de 20,90 m de anchura total, se ha subdividido en tres partes, dos simétricas respecto al eje, laterales, de 7,20 m de anchura, para la circulación en sentidos opuestos por carretera, una plataforma central, de 5 m de ancho, para el ferrocarril y dos andenes para el paso de peatones y servicios.

  3. Sobre el nombre "Quechua"

    Directory of Open Access Journals (Sweden)

    Rodolfo Cerrón-Palomino

    1985-06-01

    Full Text Available Uno de los primeros problemas con el que a menudo tropiezael estudioso de los idiomas ágrafos, como es el caso de las lenguasaborígenes de América, es el de su denominación (glotónimo.En la medida ,en que, por razones históricas, las noticias sobretales lenguas, y por consiguiente los primeros estudios sobre lasmismas, surgen a partir del contacto con los miembros de la civilización occidental, los nombres de los distintos idiomas, así comode los grupos étnicos que los manejan, son el resultado casi siempre arbitrario cie designaciones fortuitas hechas por viajeros, misiioneros y exploradores, quienes por lo general se basan, para ello, bien en descripciones físicas de los grupo étnicos, en ciertos indios geográficos que constituyen el habitat de los mismos, e incluso en prejuicios que se nutren de un marcado europocentrismo.

  4. Fome, comida e bebida na música popular brasileira: um breve ensaio

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    Francisco de Assis Guedes de Vasconcelos

    2015-09-01

    Full Text Available O artigo reflete sobre como as temáticas fome, consumo de refrigerantes e consumo de feijão com arroz são enfocadas na música popular brasileira. Investigamos os anos de ditadura militar (1964-1985. O foco da análise é a chamada canção de protesto, gênero musical caracterizado por críticas estético-cultural, político-ideológica e social aos governos militares. Tomando como referencial teórico os estudos de ideologia e filosofia da linguagem de Mikhail Bakhtin, evidenciamos que a canção de protesto retratou elementos dos contextos econômico, político e social, e propiciou a difusão de hábitos e ideologias alimentares saudáveis ou não saudáveis, contribuindo para a construção da identidade alimentar brasileira.

  5. Uma breve história de Cadernos de Saúde Pública

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    Marilia Sá Carvalho

    2015-07-01

    Full Text Available Resumo O presente artigo foi redigido para integrar o número comemorativo dos 20 anos da Ciência e Saúde Coletiva (C&SC. Aproveitamos essa oportunidade para relembrar a trajetória exitosa de Cadernos de Saúde Pública (CSP nos seus trinta anos de existência, passando de publicação trimestral de circulação restrita para um periódico mensal indexado nas principais bases de dados bibliográficas. Buscamos, adicionalmente, sintetizar alguns temas que abordamos em editoriais recentes, incluindo a internacionalização de periódicos, a demanda por artigos relevantes e originais, o plágio e a revisão por pares. Apresentamos também o sistema de gerenciamento de artigos desenvolvido por CSP e concluímos com uma reflexão sobre a cooperação entre os periódicos do campo da Saúde Coletiva.

  6. Miradas fragmentarias: sobre los desnudos en la obra fotográfica de Norma Patiño

    Directory of Open Access Journals (Sweden)

    Raymundo Mier

    2003-06-01

    Full Text Available Este texto (en realidad un conjunto de breves textos pretende una aproximación a las fotografías de Norma Patiño a partir de la conjunción de tres puntos de vista: una reflexión sobre el objeto y el acto fotográficos, una reflexión sobre la condición significativa de mirada y sus límites en la aprehensión del cuerpo con el espectro de sus sentidos, y una reflexión sobre los sentidos de la experiencia corporal y la desnudez en el dominio del erotismo. Las diversas series fotográficas de Norma Patiño, que exploran por distintas vías estas tres esferas de la significación del cuerpo y la fuerza icónica de imagen fotográfica, emplean y recrean una vastedad de recursos estéticos en la tradición los desnudos fotográficos, pero también hacen visibles sus límites, sus fracturas, hace patente las tensiones a la deriva en la aprehensión de la corporalidad y en las aporías de la representación del cuerpo y del juego de la transgresión.

  7. Algunos apuntes sobre comunidad

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    Clara Pérez Cárdenas

    2000-08-01

    Full Text Available Comunidad es, entre los temas de estudio del curriculum de los residentes en Medicina General Integral, uno de aquellos en las que mayores dificultades encontramos para poderlo impartir, pues la bibliografía sobre el tema está un tanto dispersa. Es un aspecto que los propios residentes no evalúan con justeza; sin embargo es de sumo interés que como profesionales de la salud no obviemos que nuestro trabajo de promoción, prevención y tratamiento, debe darse a nivel de individuo, pero también en las familias y en la comunidad como un todo, y por lo tanto debamos incorporar, al menos, nociones de lo que es una comunidad y qué es participación comunitaria para tener idea de cómo fomentarla, única vía para que nuestras acciones de salud sean más efectivasCommunity is one of the most difficult subjects to be taught among those included in the curriculum of General Comprehensive Medicine residents, since bibliography on this topic is a bit dispersed. This aspect, which is not properly evaluated by residents, is very important for health professionals to do our work of promotion, prevention and treatment not only at the level of the individual, but also at the level of the family and of the community as a whole. Therefore, we need to have some notions about community and community participation to be able to promote them, as the only way to make our health actions more effective

  8. LOS BENEFICIOS DE LA LECTURA COMPARTIDA DE LIBROS: BREVE REVISIÓN

    Directory of Open Access Journals (Sweden)

    Edurne Goikoetxea Iraola

    2015-01-01

    Full Text Available La lectura compartida de libros infantiles ilustrados se refiere a la lectura en voz alta que hace un adulto en compañía de un niño, generalmente prelector. Esta actividad es una de las prácticas que se tiene en cuenta en los estudios sociológicos y económicos sobre inversión parental y logro educativo. El propósito de este trabajo es revisar los beneficios de la lectura compartida y describir las posibles razones que explican estos beneficios. La investigación realizada con niños de diferente clase social y nivel de desarrollo del lenguaje ha demostrado que la lectura compartida tiene importantes efectos directos en el desarrollo del lenguaje oral y el conocimiento de lo impreso, e indirectos en la alfabetización temprana y el aprendizaje de la lectura. Se presentan los principales modelos de interacción durante la lectura: la lectura dialógica y la lectura con énfasis en lo impreso. También se analizan las características de la lectura compartida que mejor explican su beneficio tales como el hecho de tratarse de una actividad rutinaria, el rico vocabulario de los libros, el estilo de interacción entre el adulto y el niño, la experiencia emocional que genera y la exposición al texto escrito. Finalmente, se discuten algunas implicaciones educativas incluyendo orientaciones acerca de la frecuencia de la lectura compartida, la edad de inicio, las características de los libros, la función del entrenamiento y los modelos de interacción entre el adulto y el niño para el óptimo desarrollo de esta actividad tan valiosa en la alfabetización temprana y el posterior rendimiento y hábito lector.

  9. Breve examen del Tribunal de las Aguas de Valencia y de su proceso

    Directory of Open Access Journals (Sweden)

    Fairén Guillén, Víctor

    2003-07-01

    Full Text Available Se me pide, de parte de mi compañero y amigo de la Academia, Dr José M" Castán Vázquez, que elabore un pequeño trabajo para la revista «Arbor» —a cuyas líneas tuve vedado el acceso por muchos años; mientras duró aquella ordenación y aquella Nomenklatura al frente del CSIC— ; por su director actual. Prof. Dr. PEDRO GARCÍA BARRENO, al que trato casi constantemente-, Y sobre el Tribunal de las Aguas de Valencia, organismo jurídico jurisdiccional —pese a incluso enfurecidos adversarios— único, y admirado por todo el mundo, menos por quienes aspiran a transformar una de las huertas españolas mejor cuidadas, en una selva de hormigón.
    Ahí van esas líneas, resumen de un libro que, a pie de obra, me costó más de 25 años de elaboración allá en Valencia, y sin ayuda práctica de nadie sino del referido Tribunal. Desafío a que las críticas que se me hagan, se funden en observaciones directas; y no enlatadas en «ordenadores» del terreno y de sus habitantes. No admito «realidades visuales», que no son sino ficciones inmerecidamente decoradas con ese equívoco nombre, lo que ya está dando sus frutos.

  10. ¿Los Mejores Perfumes Vienen en Envases Pequeños?: Potencial de las Intervenciones Breves en el Contexto Educativo

    Directory of Open Access Journals (Sweden)

    MADELEINE BARRERA

    2015-01-01

    Full Text Available Recientemente se han desarrollado intervenciones escolares breves basadas en teorías motivacionales y de psicologíasocial. Estas intervenciones, algunas de solamente 15 minutos, han obtenido resultados positivos a largo plazo sobredominios como el desempeño académico y la convivencia escolar. Este artículo hace una revisión de sus objetivos,características, fundamentos teóricos, evaluaciones y condiciones necesarias para su diseño e implementación. En general,el objetivo de estas intervenciones breves es transformar creencias, pensamientos o sentimientos de un segmentoparticular de la población estudiantil, considerado en riesgo. Además, buscan romper círculos viciosos al intervenir enmomentos específicos de procesos recursivos. Finalmente, esta revisión analiza sus alcances, implicaciones y limitaciones,y sugiere futuras investigaciones.

  11. Bifidobacterium breve UCC2003 metabolises the human milk oligosaccharides lacto-N-tetraose and lacto-N-neo-tetraose through overlapping, yet distinct pathways

    Science.gov (United States)

    James, Kieran; Motherway, Mary O’Connell; Bottacini, Francesca; van Sinderen, Douwe

    2016-01-01

    In this study, we demonstrate that the prototype B. breve strain UCC2003 possesses specific metabolic pathways for the utilisation of lacto-N-tetraose (LNT) and lacto-N-neotetraose (LNnT), which represent the central moieties of Type I and Type II human milk oligosaccharides (HMOs), respectively. Using a combination of experimental approaches, the enzymatic machinery involved in the metabolism of LNT and LNnT was identified and characterised. Homologs of the key genetic loci involved in the utilisation of these HMO substrates were identified in B. breve, B. bifidum, B. longum subsp. infantis and B. longum subsp. longum using bioinformatic analyses, and were shown to be variably present among other members of the Bifidobacterium genus, with a distinct pattern of conservation among human-associated bifidobacterial species. PMID:27929046

  12. Parmênides e frege: um breve estudo sobre as relações entre o poema sobre a natureza e as investigações lógicas

    Directory of Open Access Journals (Sweden)

    Rafael Huguenin

    2013-06-01

    Full Text Available O presente texto tem como objetivo estabelecer algumas relações entre o poema de Parmênides e as Investigações Lógicas, de Frege. Mais especificamente, nosso objetivo é iluminar certos aspectos do poema de Parmênides por meio de uma comparação com certas noções utilizadas por Frege para caracterizar aspectos centrais de seu pensamento.The aim of this paper is to establish some relationships between Parmenides' Poem and Frege's Logical Investigations. More specifically, our objective is to illuminate some aspects of the Parmenides' Poem by comparing it to certain notions used by Frege in his Logical Investigations.

  13. Breve recopilación sobre la historia del Pueblo Gitano: desde su salida del Punjab, hasta la Constitución Española de 1978 Veinte hitos sobre la "otra" historia de España

    Directory of Open Access Journals (Sweden)

    Jesús María APARICIO GERVÁS

    2006-01-01

    Full Text Available Uno de los primeros pasos para trabajar la Educación Intercultural, no nos quepa la menor duda que es el del conocimiento de la realidad del otro. Y en este sentido nos preguntamos, ¿qué conocemos de la historia, las costumbres y las tradiciones del Pueblo Gitano? ¿No estaremos educativamente actuando a través de estereotipos y prejuicios? Pues bien, nuestro objetivo no es otro que el de intentar acercarnos a tan sólo algunos aspectos de su realidad histórica, tan escasamente conocida. Realidad que nos permita incorporarla a la propia Historia de España y que, a su vez, nos facilite, más adelante, el comprender mejor sus costumbres y sus tradiciones. En definitiva, acercarnos un paso más hacia la convivencia.

  14. Mario Bunge en la Universidad Nacional de La Plata : Breve testimonio de su visita en el año 2000

    OpenAIRE

    Bunge, Mario

    2000-01-01

    Radio Universidad Nacional de La Plata registra un breve testimonio del Dr. Mario Bunge en su visita a la UNLP en el año 2000. Resalta su paso por esta Universidad, así como las características de la misma. Mario Augusto Bunge: nació en Buenos Aires el 21 de septiembre de 1919. Físico y filósofo, se graduó en la UNLP en 1952. Reside en Montreal (Canadá).

  15. Fundamentos psicolingüísticos y psicométricos de un test breve de bilingüismo quechua-español (TBB

    Directory of Open Access Journals (Sweden)

    Raúl Gonzales Moreyra

    1985-12-01

    Full Text Available En base a trabajos de investigación previos, el autor propone un Test Breve de Bilingüismo Quechua-Español formado por reactivos gráficos. Se presenta los resultados de los análisis de validez y confiabilidad obtenidos así como el análisis de ítems y el escalograma. Todas las mencionadas mediciones permiten considerar la prueba como aceptable para futuras investigaciones.

  16. Associação entre distúrbios do ombro e trabalho: breve revisão da literatura Association between shoulder diseases and work: a brief review

    Directory of Open Access Journals (Sweden)

    Hélio Pires de Mendonça Jr.

    2005-06-01

    Full Text Available Este artigo apresenta os resultados de uma breve revisão da literatura focalizada nos distúrbios do ombro. As publicações foram pesquisadas nos sites do Medline, Ovid, Lilacs e periódicos Capes, no período de janeiro de 1993 a julho de 2004, com as seguintes palavras-chave: shoulder pain, rotator cuff, job, dor no ombro, manguito rotador, trabalho. Foram selecionados os artigos de acordo com os seguintes critérios: estudo epidemiológico publicado em inglês, português ou espanhol, com informações sobre a carga física ou sobre os fatores psicossociais do trabalho, explicitação do método de avaliação da exposição, presença de dor no ombro ou tendinopatia do manguito rotador. Vinte artigos preencheram todos os critérios de inclusão. Encontrou-se associação positiva entre os distúrbios do ombro e o trabalho praticado pelos sujeitos dos estudos revisados. Restaram como limite da revisão: a heterogeneidade dos artigos quanto à avaliação da exposição, ao tipo de diagnóstico, à metodologia utilizada na condução da pesquisa, e aos meios para avaliação da dor. Os autores discutem a necessidade de aprofundamento dos métodos de investigação de problemas musculoesqueléticos relacionados ao trabalho.This article presents the results of a brief review of shoulder diseases and their association with work. Pertinent studies, identified by the keywords shoulder pain, rotator cuff, and job were reviewed in the reference databases Medline, Ovid, Lilacs and Capes journals (January 1993 to July 2004. The articles were chosen according to the following criteria: either epidemiological or experimental surveys having been published in English, Portuguese or Spanish, with information about physical demands or psychosocial factors of work, evaluation of exposure, presence of shoulder pain or rotator cuff lesion. After the search, 20 articles were included in the revision. The articles show a relationship between shoulder diseases

  17. Bifidobacterium breve UCC2003 surface exopolysaccharide production is a beneficial trait mediating commensal-host interaction through immune modulation and pathogen protection.

    Science.gov (United States)

    Fanning, Saranna; Hall, Lindsay J; van Sinderen, Douwe

    2012-01-01

    Bifidobacteria constitute a substantial proportion of the human gut microbiota. There are currently many bifidobacterial strains with claimed probiotic attributes. The mechanism through which these strains reside within their host and exert benefits to the host is far from fully understood. We have shown in the case of Bifidobacterium breve UCC2003 that a cell surface exopolysaccharide (EPS) plays a role in in vivo persistence. Biosynthesis of two possible EPSs is controlled by a bidirectional gene cluster which guides alternate EPS synthesis by means of a reorienting promoter. The presence of EPS impacts on host immune response: the wild type, EPS-positive B. breve UCC2003 efficiently evades the adaptive B-cell host response, while its isogenic, EPS-deficient equivalent elicits a strong adaptive immune response. Functionally, EPS positive strains were more resilient to presence of acid and bile and were responsible for reduced colonization levels of Citrobacter rodentium, a gut pathogen. In conclusion, we have found that EPS is important in host interactions and pathogen protection, the latter indicative of a probiotic ability for the EPS of B. breve UCC2003.

  18. Cross-feeding by Bifidobacterium breve UCC2003 during co-cultivation with Bifidobacterium bifidum PRL2010 in a mucin-based medium.

    Science.gov (United States)

    Egan, Muireann; Motherway, Mary O'Connell; Kilcoyne, Michelle; Kane, Marian; Joshi, Lokesh; Ventura, Marco; van Sinderen, Douwe

    2014-11-25

    Bifidobacteria constitute a specific group of commensal bacteria that commonly inhabit the mammalian gastrointestinal tract. Bifidobacterium breve UCC2003 was previously shown to utilize a variety of plant/diet/host-derived carbohydrates, including cellodextrin, starch and galactan, as well as the mucin and HMO-derived monosaccharide, sialic acid. In the current study, we investigated the ability of this strain to utilize parts of a host-derived source of carbohydrate, namely the mucin glycoprotein, when grown in co-culture with the mucin-degrading Bifidobacterium bifidum PRL2010. B. breve UCC2003 was shown to exhibit growth properties in a mucin-based medium, but only when grown in the presence of B. bifidum PRL2010, which is known to metabolize mucin. A combination of HPAEC-PAD and transcriptome analyses identified some of the possible monosaccharides and oligosaccharides which support this enhanced co-cultivation growth/viability phenotype. This study describes the potential existence of a gut commensal relationship between two bifidobacterial species. We demonstrate the in vitro ability of B. breve UCC2003 to cross-feed on sugars released by the mucin-degrading activity of B. bifidum PRL2010, thus advancing our knowledge on the metabolic adaptability which allows the former strain to colonize the (infant) gut by its extensive metabolic abilities to (co-)utilize available carbohydrate sources.

  19. Myosin-cross-reactive antigen (MCRA protein from Bifidobacterium breve is a FAD-dependent fatty acid hydratase which has a function in stress protection

    Directory of Open Access Journals (Sweden)

    Ross R

    2011-02-01

    Full Text Available Abstract Background The aim of this study was to determine the catalytic activity and physiological role of myosin-cross-reactive antigen (MCRA from Bifidobacterium breve NCIMB 702258. MCRA from B. breve NCIMB 702258 was cloned, sequenced and expressed in heterologous hosts (Lactococcus and Corynebacterium and the recombinant proteins assessed for enzymatic activity against fatty acid substrates. Results MCRA catalysed the conversion of palmitoleic, oleic and linoleic acids to the corresponding 10-hydroxy fatty acids, but shorter chain fatty acids were not used as substrates, while the presence of trans-double bonds and double bonds beyond the position C12 abolished hydratase activity. The hydroxy fatty acids produced were not metabolised further. We also found that heterologous Lactococcus and Corynebacterium expressing MCRA accumulated increasing amounts of 10-HOA and 10-HOE in the culture medium. Furthermore, the heterologous cultures exhibited less sensitivity to heat and solvent stresses compared to corresponding controls. Conclusions MCRA protein in B. breve can be classified as a FAD-containing double bond hydratase, within the carbon-oxygen lyase family, which may be catalysing the first step in conjugated linoleic acid (CLA production, and this protein has an additional function in bacterial stress protection.

  20. Myosin-cross-reactive antigen (MCRA) protein from Bifidobacterium breve is a FAD-dependent fatty acid hydratase which has a function in stress protection.

    Science.gov (United States)

    Rosberg-Cody, Eva; Liavonchanka, Alena; Göbel, Cornelia; Ross, R Paul; O'Sullivan, Orla; Fitzgerald, Gerald F; Feussner, Ivo; Stanton, Catherine

    2011-02-17

    The aim of this study was to determine the catalytic activity and physiological role of myosin-cross-reactive antigen (MCRA) from Bifidobacterium breve NCIMB 702258. MCRA from B. breve NCIMB 702258 was cloned, sequenced and expressed in heterologous hosts (Lactococcus and Corynebacterium) and the recombinant proteins assessed for enzymatic activity against fatty acid substrates. MCRA catalysed the conversion of palmitoleic, oleic and linoleic acids to the corresponding 10-hydroxy fatty acids, but shorter chain fatty acids were not used as substrates, while the presence of trans-double bonds and double bonds beyond the position C12 abolished hydratase activity. The hydroxy fatty acids produced were not metabolised further. We also found that heterologous Lactococcus and Corynebacterium expressing MCRA accumulated increasing amounts of 10-HOA and 10-HOE in the culture medium. Furthermore, the heterologous cultures exhibited less sensitivity to heat and solvent stresses compared to corresponding controls. MCRA protein in B. breve can be classified as a FAD-containing double bond hydratase, within the carbon-oxygen lyase family, which may be catalysing the first step in conjugated linoleic acid (CLA) production, and this protein has an additional function in bacterial stress protection.

  1. Myosin-cross-reactive antigen (MCRA) protein from Bifidobacterium breve is a FAD-dependent fatty acid hydratase which has a function in stress protection

    LENUS (Irish Health Repository)

    Rosberg-Cody, Eva

    2011-02-17

    Abstract Background The aim of this study was to determine the catalytic activity and physiological role of myosin-cross-reactive antigen (MCRA) from Bifidobacterium breve NCIMB 702258. MCRA from B. breve NCIMB 702258 was cloned, sequenced and expressed in heterologous hosts (Lactococcus and Corynebacterium) and the recombinant proteins assessed for enzymatic activity against fatty acid substrates. Results MCRA catalysed the conversion of palmitoleic, oleic and linoleic acids to the corresponding 10-hydroxy fatty acids, but shorter chain fatty acids were not used as substrates, while the presence of trans-double bonds and double bonds beyond the position C12 abolished hydratase activity. The hydroxy fatty acids produced were not metabolised further. We also found that heterologous Lactococcus and Corynebacterium expressing MCRA accumulated increasing amounts of 10-HOA and 10-HOE in the culture medium. Furthermore, the heterologous cultures exhibited less sensitivity to heat and solvent stresses compared to corresponding controls. Conclusions MCRA protein in B. breve can be classified as a FAD-containing double bond hydratase, within the carbon-oxygen lyase family, which may be catalysing the first step in conjugated linoleic acid (CLA) production, and this protein has an additional function in bacterial stress protection.

  2. Informe preliminar sobre paludrina

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    Aquiles Peñuela Rozo

    1948-05-01

    Full Text Available En el año de 1945, Curd Davey y Rose eligieron la pirimidina como substancia de experimentación debido a que esta substancia se encuentra en las nucleo-proteinas y por su presencia en algunas sulfadrogas especial mente la sulfadiazina, de conocidas propiedades anti-palúdicas. Ensayaron la pirimidina y derivados en infestaciones por plasmodium gallinaceum en gallinas; y comprobaron que estas substancias tenían realmente acción antipalúdica. Pero al trasladar sus experiencias al hombre, encontraron que la pirimidina y sus derivados eran demasiado tóxicos. Pensando, entonces, en la similitud química del grupo de la pirimida y de la guanidina, incluyeron este último grupo en los compuestos para ensayar, descubriendo que la inclusión de la guanidina aumentaba grandemente la acción de las substancias en experiencia. Se obtuvo de esta manera un producto denominado en el numero 4-888, o paludrina, cuya fórmula o nombre químico es N. P. clorofenil N5 isopropildiguanidina, droga que se encontró muy activa contra las formas sanguíneas del Plasmodium Gallinaceum y que también se hallo activa contra las formas exoeritrocíticas del parásito. Esta capacidad de obrar sobre la fase exoeritrocítica es prácticamente exclusiva de esta substancia. Hasta hace poco tiempo esta fase exoeritrocítica, había sido demostrada solamente en trabajos de experimentación en las  aves; pero en la actualidad ha quedado también demostrada en la enfermedad humana, por experiencias del Filipino Nolasco and África, quien logro demostrar el plasmodium Vivax en el tejido retículo endotelial del pulmón de enfermos de paludismo, aunque ya Brug, en el año de 1940 había hecho comunicaciones en el mismo sentido. Se comprende la grande importancia de la paludrina, en el tratamiento del paludismo; si se considera que las recidivas de la enfermedad son debidas a la existencia de esta fase xeoeritrocítica.

  3. União Europeia: Uma breve história do futuro para um governo dos bens comuns europeu

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    António Covas

    2017-05-01

    Full Text Available A integração europeia é um bom exemplo de aplicação de uma teoria dos comuns, embora esta perspetiva não seja muito habitual. Estamos, porém, convencidos de que a via do federalismo cooperativo é uma excelente aproximação a esta teoria dos bens comuns ou colaborativa, como agora se diz. 60 anos depois do tratado de Roma e num ano, 2017, em que tudo pode acontecer, inclusive, uma “tragédia dos comuns”, decidimos que há motivos suficientes para fazer uma breve viagem ao futuro do projeto europeu e desenhar um decálogo dos “comuns europeus”, como guião para o próximo programa governativo da União Europeia. | European Union: A brief History of the Future for a government of European commons. It is not a very usual outlook, but from the political point of view, the European integration is a good application of the commons based theory. Besides, we are also convinced that the cooperative federalism method is an excellent approach to this theory. In 2017, and 60 years after the Treaty of Rome, everything seems again at stake, that is why we decided to make a small journey to the future of the European project and, for that purpose, to redesign the European commons catalogue for the next European government.

  4. Oral administration of Bifidobacterium breve attenuates UV-induced barrier perturbation and oxidative stress in hairless mice skin.

    Science.gov (United States)

    Ishii, Yuki; Sugimoto, Saho; Izawa, Naoki; Sone, Toshiro; Chiba, Katsuyoshi; Miyazaki, Kouji

    2014-07-01

    Recent studies have shown that some probiotics affect not only the gut but also the skin. However, the effects of probiotics on ultraviolet (UV)-induced skin damage are poorly understood. In this study, we aim to examine whether oral administration of live Bifidobacterium breve strain Yakult (BBY), a typical probiotic, can attenuate skin barrier perturbation caused by UV and reactive oxygen species (ROS) in hairless mice. The mice were orally supplemented with a vehicle only or BBY once a day for nine successive days. Mouse dorsal skin was irradiated with UV from days 6 to 9. The day after the final irradiation, the transepidermal water loss (TEWL), stratum corneum hydration, and oxidation-related factors of the skin were evaluated. We elucidated that BBY prevented the UV-induced increase in TEWL and decrease in stratum corneum hydration. In addition, BBY significantly suppressed the UV-induced increase in hydrogen peroxide levels, oxidation of proteins and lipids, and xanthine oxidase activity in the skin. Conversely, antioxidant capacity did not change regardless of whether BBY was administered or not. In parameters we evaluated, there was a positive correlation between the increase in TEWL and the oxidation levels of proteins and lipids. Our results suggest that oral administration of BBY attenuates UV-induced barrier perturbation and oxidative stress of the skin, and this antioxidative effect is not attributed to enhancement of antioxidant capacity but to the prevention of ROS generation.

  5. Cloning and characterization of ginsenoside Ra1-hydrolyzing beta-D-xylosidase from Bifidobacterium breve K-110.

    Science.gov (United States)

    Hyun, Yang-Jin; Kim, Bomi; Kim, Dong-Hyun

    2012-04-01

    beta-D-Xylosidase (E.C. 3.2.1.37) from Bifidobacterium breve K-110, which hydrolyzes ginsenoside Ra1 to ginsenoside Rb2, was cloned and expressed in Escherichia coli. The (His6)-tagged recombinant enzyme, designated as XlyBK- 110, was efficiently purified using Ni²⁺-affinity chromatography (109.9-fold, 84% yield). The molecular mass of XylBK- 100 was found to be 55.7 kDa by SDS-PAGE. Its sequence revealed a 1,347 bp open reading frame (ORF) encoding a protein containing 448 amino acids, which showed 82% identity (DNA) to the previously reported glycosyl hydrolase family 30 of Bifidobacterium adolescentis ATCC 15703. The Km and Vmax values toward p-nitrophenyl-beta-D-xylopyranoside (pNPX) were 1.45mM and 10.75 micromol/min/mg, respectively. This enzyme had pH and temperature optima at 6.0 and 45 degrees C, respectively. XylBK-110 acted to the greatest extent on xyloglucosyl kakkalide, followed by pNPX and ginsenoside Ra1, but did not act on p-nitrophenyl-alpha-Larabinofuranoside, p-nitrophenyl-beta-D-glucopyranoside, or p-nitrophenyl-beta-D-fucopyranoside. In conclusion, this is the first report on the cloning and expression of beta-Dxylosidase- hydrolyzing ginsenoside Ra1 and kakkalide from human intestinal microflora.

  6. Transcriptional and functional characterization of genetic elements involved in galacto-oligosaccharide utilization by Bifidobacterium breve UCC2003

    Science.gov (United States)

    O'Connell Motherway, Mary; Kinsella, Michael; Fitzgerald, Gerald F; Sinderen, Douwe

    2013-01-01

    Several prebiotics, such as inulin, fructo-oligosaccharides and galacto-oligosaccharides, are widely used commercially in foods and there is convincing evidence, in particular for galacto-oligosaccharides, that prebiotics can modulate the microbiota and promote bifidobacterial growth in the intestinal tract of infants and adults. In this study we describe the identification and functional characterization of the genetic loci responsible for the transport and metabolism of purified galacto-oligosaccharides (PGOS) by Bifidobacterium breve UCC2003. We further demonstrate that an extracellular endogalactanase specified by several B. breve strains, including B. breve UCC2003, is essential for partial degradation of PGOS components with a high degree of polymerization. These partially hydrolysed PGOS components are presumed to be transported into the bifidobacterial cell via various ABC transport systems and sugar permeases where they are further degraded to galactose and glucose monomers that feed into the bifid shunt. This work significantly advances our molecular understanding of bifidobacterial PGOS metabolism and its associated genetic machinery to utilize this prebiotic. PMID:23199239

  7. Las tablas, breves “diccionarios morales” en la edición de obras del XVII

    Directory of Open Access Journals (Sweden)

    Beatriz Gutiérrez Mueller

    2015-03-01

    Full Text Available A mediados del siglo XVI, se inició la costumbre de agregar un índice, más comúnmente llamado tabla, colocado por lo común al final del volumen impreso como guía de lectura y que constaba de unos pocos folios sin numeración. Para mediados del XVII, sin embargo, las tablas se fueron convirtiendo en abultados resúmenes de lo que contenía un libro y, en algunos casos, en lo que podría denominarse “breves diccionarios morales”, como en el volumen V de las Obras completas de Juan de Palafox y Mendoza, de 1665. Si bien muchas fueron escuetas, hubo casos de tablas narrativas, como la que aparece en una obra de Juan Bautista Poza [1647]. En Arte de predicar la palabra de Dios [1667], incluso, se ofrece una manera de hacerlas. En este artículo podrá apreciarse que existía un criterio editorial moralizante en las prensas españolas del Barroco, a la hora de seleccionar qué contenidos debían indizarse, sin dejar de ser las tablas, como hasta nuestros días, un lugar útil que facilita la búsqueda de un tema.

  8. Ensaios sobre economia do empreendorismo

    OpenAIRE

    Gabrielito Rauter Menezes

    2015-01-01

    Esta tese é composta por três ensaios sobre Economia do Empreendedorismo. O primeiro deles trata sobre os determinantes do empreendedorismo no Brasil a partir de modelos de escolha ocupacional, usando os microdados da Pesquisa Nacional por Amostra por Domicílios (PNAD) do ano de 2012. A estratégia empírica adotada empregou os modelos de escolha discreta na estimação da escolha ocupacional. Os resultados demonstraram que existem efeitos significativos para as variáveis: anos de estudos iniciai...

  9. Doce magnos oradores: Breves consideraciones biográficas e intento de acercamiento a uno de sus discursos

    OpenAIRE

    JOSELYN CORREDOR TAPIAS

    2012-01-01

    Este artículo ofrece una visión general sobre la vida de doce grandes oradores. En primera instancia, se presentan unas consideraciones teóricas sobre su biografía; posteriormente, se da a conocer uno de los discursos más famosos de algunos de los oradores escogidos. En el caso de otros, solo se presenta un fragmento; luego, se exponen algunas de sus frases célebres. Respecto de dos oradores, infortunadamente, no se presenta el texto del discurso debido a que no se halló –al menos hasta el mo...

  10. Conversaciones sobre el litoral cordillerano

    OpenAIRE

    Browne,Enrique; Browne,Tomás

    2009-01-01

    Dos arquitectos dialogan sobre los Andes desde su propia vivencia. El territorio de Chile se entiende como límite natural entre mar y cordillera, donde suceden apretadamente una serie de eventos particulares: fallas geológicas, cerros que penetran en las ciudades y torrentes que bajan al mar caracterizan y diferencian sus paisajes.

  11. Sobre Humanismo y Medicina

    Directory of Open Access Journals (Sweden)

    Adolfo de Francisco Zea

    2000-12-01

    Full Text Available

    En esta disertación sobre “Humanismo y Medicina”, título del libro que tuve ocasión de publicar hace un par de años, destacaré algunas de las relaciones que existen entre la medicina y las bellas artes, concretamente la música y la pintura. La Medicina tiene como objeto de su acción al Hombre. Definida como la ciencia y el arte de prevenir, curar o aliviar la enfermedad, no tiene como meta la simple prolongación de la vida biológica.

    Su tarea consiste en postergar la muerte, prevenir o reducir los padecimientos y las deformidades, curar las enfermedades, ampliar las capacidades biológicas y psicológicas de los seres humanos y ocuparse de los sufrimientos de las personas. Para Tristam Engelhardt, es la Medicina de las personas.

    Se concibe a la persona humana como el individuo de conciencia reflexiva normal que es a la vez miembro pleno de la comunidad, con los deberes y derechos que presuponen su pertenencia a ella. A esta acepción de carácter moral de la persona humana, que corresponde a lo que se llama persona “en sentido estricto”, se agrega la acepción social, que hace relación con aquellas personas a las que se otorgan prácticamente los mismos derechos, como es el caso de los niños, de los individuos que perdieron el carácter de personas por padecer de lesiones cerebrales o demencia, y de aquellos que nunca llegarán a serlo como los retrasados mentales profundos.

    Al sostener que no todos los seres humanos son personas “en sentido estricto”, la Bioética que expone Engelhardt impone, sin embargo, como obligación moral, tratar a estos últimos con pleno respeto y comprensión. Lo que es importante acerca de nosotros mismos como seres humanos, es el hecho real de que seamos personas; no simplemente nuestra pertenencia al género Horno sapiens como tal.

    Para Víctor Frankl, la persona humana es de carácter espiritual y en tal virtud, es diferente del organismo psicofísico cuya

  12. Breves considerações acerca da poesia, da filosofia e da crítica literária

    Directory of Open Access Journals (Sweden)

    Affonso Henrique Vieira da Costa

    2016-07-01

    Full Text Available O trabalho se constitui diante de uma indagação: É possível falar sobre a arte? Ele tem por objetivo tecer considerações em torno da relação existente entre poesia, filosofia e crítica literária.

  13. Estudios sobre Plantas Andinas, X

    Directory of Open Access Journals (Sweden)

    Cuatrecasas José

    1967-09-01

    Full Text Available Durante la preparación del género Baccharis para Prima Flora Colombiana tuve que estudiar gran número de especies andinas, tropicales y extratropicales de fuera de Colombia; el objeto principal de tales estudios fue el de tipificar las especies íntimamente relacionadas con las colombianas y establecer su diferenciación taxonómica. La consulta de los tipos específicos y la identificación de gran número de colecciones procedentes de las regiones andinas hasta el sur del continente americano permitió precisar el concepto de bastantes especies y de su área geográfica, al mismo tiempo que el establecimiento de numerosas sinonimias.  La mayor parte de las novedades taxonómicas y de los comentarios derivados de tal estudio va incluída en el texto de las Astereae de Colombia a publicar en breve. El objeto de este artículo es dar a conocer otra parte de las novedades relativas a especies andinas de los países vecinos y describir varias entidades taxonómicas deficientemente conocidas, incorporando a su conocimiento los resultados de muchas disecciones llevadas a cabo en los mencionados estudios.  El trabajo basico para estas notas fue hecho en la Smithsonian Institution; se consultaron también las colecciones de los herbarios europeos de Londres, París, Florencia, Ginebra y Madrid en viaje hecho en otoño de 1963. National Science Foundation de Washington D.C. subvencionó los trabajos.

  14. A PEC 65 E O PLANEJAMENTO URBANO NO BRASIL: Um recorte sobre a inobservância dos direitos da sociedade civil em questões de meio ambiente nas estratégias de governo

    Directory of Open Access Journals (Sweden)

    Ingrid Elena Schnoor

    2017-02-01

    Full Text Available A Avaliação de Impacto Ambiental (AIA é o instrumento-modelo vigente que auxilia no planejamento e na tomada de decisão sobre empreendimentos que impactem negativamente o meio ambiente. É um instrumento técnico-racional, a ser aplicado sobre situações complexas. Mesmo sendo vital para a gestão da ação antrópica sobre os recursos e sobre o meio ambiente no planeta, por sua idade, (surgiu nos anos 60 do século XX, está, ainda, em processo de desenvolvimento e amadurecimento. E, por seu caráter regulador que impõe dificuldades ao avanço de empreendimentos econômicos, incomoda. Se a forma de avaliar (linear e contínua não dialoga com os problemas decorrentes (complexos, isto pode levar a um resultado tendencioso e incompleto, passível de questionamento sobre sua eficácia. É o que se tem visto. Este artigo expõe a Proposta de Emenda Constitucional, PEC 65/2012, em tramitação para aprovação, cujo texto tenta anular a eficácia da AIA e calar a voz da sociedade, fazendo um breve recorte histórico para mostrar os riscos de tal aprovação se concretizar.

  15. Dietary, nondigestible oligosaccharides and Bifidobacterium breve M-16V suppress allergic inflammation in intestine via targeting dendritic cell maturation.

    Science.gov (United States)

    de Kivit, Sander; Kostadinova, Atanaska I; Kerperien, JoAnn; Morgan, Mary E; Muruzabal, Veronica Ayechu; Hofman, Gerard A; Knippels, Leon M J; Kraneveld, Aletta D; Garssen, Johan; Willemsen, Linette E M

    2017-07-01

    Dietary intervention with short-chain galacto-oligosaccharides (scGOS), long-chain fructo-oligosaccharides (lcFOS) and Bifidobacterium breve M-16V ( Bb ) (GF/ Bb ) suppresses food allergic symptoms in mice, potentially via intestinal epithelial cell (IEC)-derived galectin-9. Furthermore, in vitro studies showed galacto- and fructo-oligosaccharides (GF) to enhance the immunomodulatory capacity of a TLR9 ligand representing bacterial CpG DNA when exposed to IEC. In this study, we investigated whether GF/ Bb modulates dendritic cells (DCs) and subsequent Th2 and regulatory T cell (T reg ) frequency in the small intestinal lamina propria (SI-LP). BALB/c mice were fed GF/ Bb during oral OVA sensitization. DC and T cell phenotype were determined in SI-LP mononuclear cells using flow cytometry. Murine bone marrow-derived DCs (BMDCs) were exposed to recombinant galectin-9 or human monocyte-derived DCs (moDCs) and were cultured in IEC-conditioned medium from GF and TLR9 ligand-exposed HT-29 cells. GF/ Bb reduced allergic symptoms and enhanced serum galectin-9 levels, while suppressing activation, restoring phagocytic capacity, and normalizing CD103 expression of SI-LP DCs of OVA-allergic mice. In vitro, galectin-9 suppressed LPS-induced activation markers and cytokine secretion by BMDCs, and IEC-conditioned medium suppressed moDC activation in a galectin-9-dependent manner. Besides suppression of SI-LP DC activation, dietary GF/ Bb also lowered the frequency of activated Th2 cells, while enhancing T reg in the SI-LP of OVA-allergic mice compared to the control diet. Dietary intervention with GF/ Bb enhances galectin-9 and suppresses allergic symptoms of OVA-allergic mice in association with reduced intestinal DC and Th2 activation and increased T reg frequency in these mice. © Society for Leukocyte Biology.

  16. Impiego di materiali plastici nell'industria alimentare: rilascio di 'Endocrine Disruptors' valutato mediante test a breve termine in vitro

    Directory of Open Access Journals (Sweden)

    D. Reali

    2003-05-01

    Full Text Available

    Obiettivi: valutazione del rischio di esposizione della popolazione a composti chimici estrogenomimetici rilasciati da materiali plastici utilizzati nell’industria delle bevande e nel packaging di alimenti

    Metodi: messa a punto di un metodo di estrazione di composti organici da flussi acquosi tramite l’uso di resine di amberlite® XAD®-2 con passaggio di matrice acquosa (50ml/min attraverso tubi di adduzione. L’analisi chimica di eventuali composti chimici migranti da tubi e contenitori per bevande è stata effettuata con GC/MS e arricchimento con tecnica SPME. L’attività estrogeno mimetica degli estratti è stata saggiata con un test biologico a breve termine, il Yeast Estrogen Screen. Il costrutto di Saccharomyces cerevisiae è vettore di sequenze di DNA che codificano il recettore estrogenico alfaumano e i rispettivi response elements legati alla regione promotrice del gene reporter LacZ per la ßgalattosidasi. L’induzione di sintesi di ß-galattosidasi dipende dall’attivazione del gene reporter conseguente all’affinità di legame ligando-recettore e viene misurata spettrofotometricamente. La potenza estrogenica viene calcolata in riferimento all’attività elicitata dall’ormone naturale 17ß-estradiolo nelle stesse condizioni sperimentali preso come controllo positivo di riferimento (RIE.

    Risultati preliminari e Conclusioni: l’analisi tossicologica in vitro ha evidenziato che nell’acqua che è stata a contatto con silicone e PVC in condizioni dinamiche sono presenti composti chimici (1-10 μg/lt: con attività estrogeno-mimetica (RIE= 87.31;44.28. Sono emersi aspetti interessanti da studiare ulteriormente nell’ottica della promozione della sicurezza alimentare.

  17. Congresos internacionales sobre la enseñanza de la estadística: una visión histórica y de contenido

    OpenAIRE

    Ospina Botero, David; Vasco Uribe, Carlos E.

    2009-01-01

    Los autores hacen un breve reseña histórica de los cuatro congresos internacionales de la enseñanza de la estadística (ICOTS). Con base en los temas tratados en ellos especialmente en los dos últimos, clasifican los dos tópicos más actuales del debate sobre la enseñanza de la estadística y la teoría de las probabilidades. Entre los ya tradicionales, reseñan la enseñanza de esos temas en la educación básica y en la universidad, la formación de los profesores, el uso de los computadores y de...

  18. Estudo comparativo sobre representações visuais em interfaces com base em ícones de dispositivos móveis da Apple

    OpenAIRE

    Marshal Becon Lauzer

    2013-01-01

    Esta dissertação teve por objetivo verificar se existe um diferencial estético nas representações visuais nas interfaces gráficas dos dispositivos móveis da Apple e, se existir, se esse diferencial efetivamente influencia o vínculo do usuário com a marca e se o usuário tem consciência dele. Para tanto é apresentada uma breve análise diacrônica sobre as interfaces computacionais, bem como uma descrição de momentos chave na história da referida empresa. A partir disto, são abordadas questões qu...

  19. ALGUMAS CONSIDERAÇÕES SOBRE O ZONEAMENTO URBANO: O EXEMPLO DO MUNICIPIO DE GANDU-BA

    Directory of Open Access Journals (Sweden)

    Ecirio Barreto Santos de Oliveira

    2012-06-01

    Full Text Available Resumo O presente trabalho tem como objetivo fazer uma breve análise sobre o zoneamento do espaço urbano do Município de Gandu. Esse debate sobre o zoneamento no contexto do planejamento urbano foi enfatizado a partir da ótica das Ciências Sociais, mas precisamente por meio da análise geográfica. Para tanto, os procedimentos metodológicos utilizados para a materialização desse trabalho pautaram-se basicamente no levantamento de referenciais teóricos, com base na análise de obras que trabalham com este tema e a pesquisa cartográfica. Posteriormente, realizou-se o levantamento documental, no qual se realizou visita a Secretária de Infraestrutura Urbana do Município de Gandu para coletar o Plano Diretor Urbano dessa supracitada unidade federativa. Após isso, analisou o mapa do zoneamento urbano contido no Plano Diretor Municipal de Gandu, no qual ficou constatado que o zoneamento do espaço urbano de Gandu é de cunho funcionalista.

  20. El género y el futuro de los estudios sobre el genocidio y la prevención

    Directory of Open Access Journals (Sweden)

    Elisa von Joeden-Forgey

    2016-12-01

    Full Text Available Este artículo aborda los aportes de la investigación sobre género para la definición del delito de genocidio y para aprehenderlo como proceso histórico. Se afirma que la violencia de género es un elemento central de aquel crimen. Más allá de los debates sobre la violencia y la violación sexual, se argumenta que abordar estas atrocidades en general desde una perspectiva de género brinda herramientas importantes para un sistema de advertencia que debe ser incorporado a la metodología de investigación y a las estrategias de confección de informes de las Naciones Unidas, la Corte Internacional de Justicia (CPI, las organizaciones de derechos humanos y los organismos gubernamentales y servicios de inteligencia. Por medio de un breve análisis de los casos de Darfur y Srebrenica, el artículo demuestra el modo en que las conceptualizaciones que no incorporan la dimensión de género no logran reconocer y describir de manera adecuada los tipos específicos de violencia que son, en general, indicadores del crimen de genocidio. Además, se propone que esta deficiencia puede contribuir o perpetuar, sin advertirlo, las estrategias de negación de los genocidios.

  1. Reflexiones críticas y autocríticas sobre Pedagogía Crítica

    Directory of Open Access Journals (Sweden)

    Paz Gimeno Lorente

    2013-01-01

    Full Text Available En este breve texto, hemos querido exponer la visión que, desde Fedicaria-Aragón, tenemos sobre la situación actual del pensamiento crítico en nuestro sistema educativo. En torno a esta cuestión, se han generado algunas falacias que deseamos apuntar, ya que una educación crítica no puede desarrollarse nunca en un contexto escolar apoyado sobre unos valores hegemónicos que contradicen la finalidad de formar a unos ciudadanos críticos y rebeldes frente a las situaciones de injusticia. En este texto señalamos algunos de los condicionantes que socavan esta orientación escolar crítica, desde los que corresponden a un sistema educativo colonizado por el sistema económico, hasta los bucles político-educativos que han entrelazado una ideología seudoprogresista y una práctica neoconservadora, y que, en la actualidad, han derivado en una «plana» realidad capitalista. A pesar de ello, desde Fedicaria, pretendemos mantener una «resistencia» crítica minoritaria, espacio simbólico donde se ubica el germen de los cambios sociales.

  2. Bifidobacterium breve with α-linolenic acid and linoleic acid alters fatty acid metabolism in the maternal separation model of irritable bowel syndrome.

    Science.gov (United States)

    Barrett, Eoin; Fitzgerald, Patrick; Dinan, Timothy G; Cryan, John F; Ross, R Paul; Quigley, Eamonn M; Shanahan, Fergus; Kiely, Barry; Fitzgerald, Gerald F; O'Toole, Paul W; Stanton, Catherine

    2012-01-01

    The aim of this study was to compare the impact of dietary supplementation with a Bifidobacterium breve strain together with linoleic acid & α-linolenic acid, for 7 weeks, on colonic sensitivity and fatty acid metabolism in rats. Maternally separated and non-maternally separated Sprague Dawley rats (n = 15) were orally gavaged with either B. breve DPC6330 (10(9) microorganisms/day) alone or in combination with 0.5% (w/w) linoleic acid & 0.5% (w/w) α-linolenic acid, daily for 7 weeks and compared with trehalose and bovine serum albumin. Tissue fatty acid composition was assessed by gas-liquid chromatography and visceral hypersensitivity was assessed by colorectal distension. Significant differences in the fatty acid profiles of the non-separated controls and maternally separated controls were observed for α-linolenic acid and arachidonic acid in the liver, oleic acid and eicosenoic acid (c11) in adipose tissue, and for palmitoleic acid and docosahexaenoic acid in serum (pbreve DPC6330 to MS rats significantly increased palmitoleic acid, arachidonic acid and docosahexaenoic acid in the liver, eicosenoic acid (c11) in adipose tissue and palmitoleic acid in the prefrontal cortex (pbreve DPC6330 to non separated rats significantly increased eicosapentaenoic acid and docosapentaenoic acid in serum (pbreve DPC6330 in combination with linoleic acid and α-linolenic acid to maternally separated rats significantly increased docosapentaenoic acid in the serum (pbreve DPC6330 with fatty acid supplementation to non-separated rats significantly increased liver and serum docosapentaenoic acid (pbreve DPC6330 influenced host fatty acid metabolism. Administration of B. breve DPC6330 to maternally separated rats significantly modified the palmitoleic acid, arachidonic acid and docosahexaenoic acid contents in tissues. The effect was not observed in non-separated animals.

  3. Bifidobacterium breve and Lactobacillus rhamnosus treatment is as effective as budesonide at reducing inflammation in a murine model for chronic asthma.

    Science.gov (United States)

    Sagar, Seil; Morgan, Mary E; Chen, Si; Vos, Arjan P; Garssen, Johan; van Bergenhenegouwen, Jeroen; Boon, Louis; Georgiou, Niki A; Kraneveld, Aletta D; Folkerts, Gert

    2014-04-16

    Asthma is estimated to affect as many as 300 million people worldwide and its incidence and prevalence are rapidly increasing throughout the world, especially in children and within developing countries. Recently, there has been a growing interest in the use of potentially beneficial bacteria for allergic diseases. This study is aimed at exploring the therapeutic effects of long-term treatment with two different beneficial bacterial strains (Bifidobacterium breve M-16 V and Lactobacillus rhamnosus NutRes1) and a glucocorticoid (budesonide), as a reference treatment, on inflammatory response in a murine model for chronic allergic asthma. To mimic the chronic disease in asthmatic patients, we used the murine ovalbumin-induced asthma model combined with prolonged allergen exposure. Airway function; pulmonary airway inflammation; airway remodelling, mRNA expression of pattern recognition receptors, Th-specific cytokines and transcription factors in lung tissue; mast cell degranulation; in vitro T cell activation; and expression of Foxp3 in blood Th cells were examined. Lactobacillus rhamnosus reduced lung resistance to a similar extent as budesonide treatment in chronically asthmatic mice. Pulmonary airway inflammation, mast cell degranulation, T cell activation and airway remodelling were suppressed by all treatments. Beneficial bacteria and budesonide differentially modulated the expression of toll-like receptors (TLRs), nod-like receptors (NLRs), cytokines and T cell transcription factors. Bifidobacterium breve induced regulatory T cell responses in the airways by increasing Il10 and Foxp3 transcription in lung tissue as well as systemic by augmenting the mean fluorescence intensity of Foxp3 in blood CD4+ T cells. These findings show that Bifidobacterium breve M-16 V and Lactobacillus rhamnosus NutRes1 have strong anti-inflammatory properties that are comparable to budesonide and therefore may be beneficial in the treatment of chronic asthma.

  4. Early gut colonization by Bifidobacterium breve and B. catenulatum differentially modulates eczema risk in children at high risk of developing allergic disease.

    Science.gov (United States)

    Ismail, Intan H; Boyle, Robert J; Licciardi, Paul V; Oppedisano, Frances; Lahtinen, Sampo; Robins-Browne, Roy M; Tang, Mimi L K

    2016-12-01

    An altered compositional signature and reduced diversity of early gut microbiota are linked to development of allergic disease. We investigated the relationship between dominant Bifidobacterium species during the early post-natal period and subsequent development of allergic disease in the first year of life. Faecal samples were collected at age 1 week, 1 month and 3 months from 117 infants at high risk of allergic disease. Bifidobacterium species were analysed by quantitative PCR and terminal restriction fragment length polymorphism. Infants were examined at 3, 6 and 12 months, and skin prick test was performed at 12 months. Eczema was diagnosed according to the UK Working Party criteria. The presence of B. catenulatum at 3 months was associated with a higher risk of developing eczema (OR adj = 4.5; 95% CI: 1.56-13.05, p adj = 0.005). Infants colonized with B. breve at 1 week (OR adj = 0.29; 95% CI: 0.09-0.95, p adj = 0.04) and 3 months (OR adj = 0.15; 95% CI: 0.05-0.44, p adj = 0.00001) had a reduced risk of developing eczema. Furthermore, the presence of B. breve at 3 months was associated with a lower risk of atopic sensitization at 12 months (OR adj = 0.38; 95% CI: 0.15-0.98, p adj = 0.05). B. breve colonization patterns were influenced by maternal allergic status, household pets and number of siblings. Temporal variations in Bifidobacterium colonization patterns early in life are associated with later development of eczema and/or atopic sensitization in infants at high risk of allergic disease. Modulation of the early microbiota may provide a means to prevent eczema in high-risk infants. © 2016 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.

  5. Intervención breve para adolescentes que inician el consumo abusivo de alcohol y otras drogas: su eficacia en un escenario clínico

    Directory of Open Access Journals (Sweden)

    Carlos Augusto Rodríguez Durán

    2017-06-01

    Full Text Available Introducción: en las últimas décadas, se ha observado en los adolescentes mexicanos un incremento en la cantidad y frecuencia del consumo de alcohol y drogas ilícitas; los programas de intervención breve han demostrado ser eficaces para reducir el consumo y abuso de sustancias en diversas poblaciones. Objetivo: determinar la eficacia del Programa de Intervención Breve para Adolescentes que Inician el Consumo Abusivo de Alcohol y otras Drogas (PIBA, así como el mantenimiento del cambio en los jóvenes que asistieron. Metodo: se utilizó un diseño de medidas repetidas, no probabilístico y sin grupo control. En el seguimiento participaron 60 usuarios consumidores de sustancias de entre 16 y 20 años de edad, de ambos sexos, habitantes de la Ciudad de México. Resultados: al comparar la preintervención vs. el seguimiento, se encontró que los adolescentes que participaron en el PIBA mantuvieron una reducción en su patrón de consumo (cantidad, frecuencia y tiempo de abstinencia de alcohol, mariguana e inhalables; disminuyeron los problemas asociados con el consumo; e incrementaron su nivel de autoeficacia percibida. La mejoría fue mayor en quienes concluyeron la intervención breve que en quienes no terminaron todas las sesiones. Conclusiones: el PIBA fue coadyuvante en la consecución de las metas propuestas por la mayoría de los adolescentes que asistieron a este tratamiento psicológico.

  6. Regulation on power efficiency in the automobile; Regulacion sobre eficiencia energetica en el automovil

    Energy Technology Data Exchange (ETDEWEB)

    Maldonado Susano, Armando; Romero de Vivar Uvaldo, Pascual [Comision Nacional para el Ahorro de Energia, Mexico, D.F. (Mexico)

    2001-07-01

    In this article it is presented a brief analysis of one of the regulations on the yield of fuel consumption of the light vehicles that promoted substantial changes in the Mexican automotive industry, the decree denominated PREMCE (Average of minimum fuel yield by company), as well as some international experiences relative to the subject. It is also indicated the evolution, in the last years, of the average yields of fuel consumption (Km/L) in Mexico in the new automobiles and the more important technological factors that affect their energy efficiency. [Spanish] En este articulo se presenta un breve analisis de una de las regulaciones sobre el rendimiento de consumo de combustible de los vehiculos ligeros que promovio cambios sustanciales en la industria automotriz mexicana, el decreto denominado Premce (Promedio de rendimiento minimo de combustible por empresa), asi como algunas experiencias internacionales relativas al tema. Se senala tambien la evolucion, en los ultimos anos, de los rendimientos promedio de consumo de combustible (Km/L) en Mexico en los automoviles nuevos y los factores tecnologicos mas importantes que influyen en su eficiencia energetica.

  7. REFLEXÕES SOBRE MODERNIDADE, TURISMO E CAMPO SOCIAL NO ESTADO DE ALAGOAS - BRASIL

    Directory of Open Access Journals (Sweden)

    Daniel Arthur Lisboa de Vasconcelos

    2013-02-01

    Full Text Available O presente artigo, de caráter ensaístico, visa a contribuir com reflexões sobre modernidade, turismo e campo social sociais no Estado de Alagoas - Brasil. Utilizando o referencial de autores como Giddens (2001, Jameson (2002 e Harvey (1992, dentre outros, sintetizaremos algumas características das transições socioculturais da modernidade para o atual estágio do modo de produção capitalista, período que muitos têm considerado como a “pós-modernidade”. Posteriormente, empreenderemos breve descrição crítica acerca do atual modelo de turistificação no Estado, com base nos conceitos de Habitus e Campo Social, de Pierre Bourdieu. Conclui-se que o turismo em Alagoas está ancorado em roteiros que, em sua dominância, têm se consolidado sob os enunciados de Sol e Mar, tendência natural do turismo de massa que ocorre nas regiões intertropicais do mundo inteiro, em caso mais especifico, no Nordeste Brasileiro, e que também sofre direta influência da formação histórica de uma cultura de alheamento às potencialidades dos patrimônios identitários locais.

  8. Governança na internet: um estudo sobre o Marco Civil brasileiro

    Directory of Open Access Journals (Sweden)

    Laura Vilela Rodrigues Rezende

    2016-01-01

    Full Text Available El trabajo tiene como propósito realizar un estudio sobre la legislación que rige el uso de Internet en Brasil, en el Marco Civil de Internet (Ley 12.965/2014. Se trata de una investigación descriptiva, de naturaleza cua litativa, basada en recopilación bibliográfica y estudio comparativo. Se ex pone inicialmente un breve histórico de Internet en Brasil y su panorama actual. Luego, se presenta el concepto de gobernanza en Internet y los su cesos que marcaron las discusiones acerca de esta temática. Se aborda el Marco Civil de Internet en Brasil, el histórico de su creación y los principios que lo rigen. Se concluye que es un importante instrumento oficial regla mentario para asegurar la libertad y los derechos del usuario. Su concep ción se fundamentó en la participación de la sociedad civil, lo que puede considerarse inédito en lo que se refiere a la creación de leyes reglamenta rias. Se considera que esta sea una ley sofisticada en cuanto a sus directri ces, lo que la vuelve una de las más progresistas del mundo. Pese a todo lo anterior, su trayectoria democrática de creación le otorga un carácter ge neralista y superficial.

  9. Medicina antienvelhecimento: notas sobre uma controvérsia sociotécnica

    Directory of Open Access Journals (Sweden)

    Antônio Nogueira Leitão

    2014-12-01

    Full Text Available Após algumas décadas de batalha, a geriatria e a gerontologia se tornaram as legítimas ciências do envelhecimento. Hoje surge uma contestação a tal condição. Em sua breve história, a medicina antienvelhecimento se afirmou como prática médica que questiona o modo de se endereçar o envelhecimento biológico. Com isso, toda a medicina é questionada. Aqui, exploramos especialmente como essa controvérsia se estrutura em torno dos fundamentos das ciências do envelhecimento. Há bases para esses questionamentos? Como eles foram tratados por aqueles que os receberam? Tendo em vista uma perspectiva sociotécnica, é interessante pensar que, para geriatras e gerontólogos, a necessária crítica à medicina antienvelhecimento também traz uma importante reflexão sobre o modo como as ciências do envelhecimento vêm tratando seu objeto.

  10. Tendencias actuales en la investigación sobre corrosión de armaduras

    Directory of Open Access Journals (Sweden)

    Andrade, Carmen

    1988-12-01

    Full Text Available During the last 20 years, the corrosion of concrete rebars is increasily being considered a major problem due the high costs spent by some Governments on repair and of civil concrete structures. In the present paper, a brief description on the causes of this type of corrosion is offered together with some references to the costs of repairing of bridge decks published by USA researchs. Finally, the main lines of research which, in opinion of the authors have priority, are commented.

    El fenómeno de la corrosión de armaduras en hormigón, en los últimos 20 años, ha pasado a ser un problema económico de primera magnitud por los costos que supone para algunos Gobiernos la reparación y el mantenimiento de muchas Obras Públicas. En el presente artículo se hacen unas breves referencias a las causas más frecuentes que provocan esta corrosión y se dan algunas cifras ofrecidas por investigadores norteamericanos sobre los costos en reparación de puentes de hormigón armado. Finalmente, se comentan las líneas de investigación que, a juicio de los autores, son prioritarias en esta materia.

  11. Evaluación breve del lenguaje oral en adultos mediante la batería de neuropsicología cognitiva: NEUROBEL

    OpenAIRE

    Adrian-Torres, Jose Antonio

    2015-01-01

    La batería NEUROBEL (Batería breve de evaluación del lenguaje oral en adultos) es una nueva prueba de evaluación de sondeo (screening), que ha sido publicada recientemente en España con los valores de un estudio piloto pensada para profesionales que necesiten una valoración, en un corto periodo de tiempo, de los principales procesos implicados en el lenguaje oral desde paradigmas psicolingüísticos. La batería NEUROBEL puede considerarse una versión abreviada de otras baterías más largas de ev...

  12. Validez de la escala breve de Zung para tamizaje del episodio depresivo mayor en la población general de Bucaramanga, Colombia

    OpenAIRE

    Campo, Adalberto; Díaz, Luis Alfonso; Rueda, Germán Eduardo

    2006-01-01

    Introducción. Las escalas breves para identificar trastornos depresivos conservan la utilidad de las escalas extensas como instrumentos para tamizaje. Sin embargo, no se cuenta con una escala de estas características validada en población general colombiana. Objetivo. Diseñar una escala abreviada de la escala de Zung para depresión para tamizaje de episodio depresivo mayor en adultos residentes en la comunidad general. Materiales y métodos. A partir de la aplicación de la escala de Zung de ve...

  13. Validez de la escala breve de Zung para tamizaje del episodio depresivo mayor en la población general de Bucaramanga, Colombia

    OpenAIRE

    Adalberto Campo; Luis Alfonso Díaz; Germán Eduardo Rueda

    2006-01-01

    Introducción. Las escalas breves para identificar trastornos depresivos conservan la utilidad de las escalas extensas como instrumentos para tamizaje. Sin embargo, no se cuenta con una escala de estas características validada en población general colombiana. Objetivo. Diseñar una escala abreviada de la escala de Zung para depresión para tamizaje de episodio depresivo mayor en adultos residentes en la comunidad general. Materiales y métodos. A partir de la aplicación de la escala de Zung...

  14. Validación inicial de una Escala Breve de Satisfacción con los Estudios en estudiantes universitarios de Lima

    OpenAIRE

    Merino-Soto, César; Dominguez-Lara, Sergio; Fernández-Arata, Manuel

    2017-01-01

    Objetivo: Realizar el análisis psicométrico preliminar de la Escala Breve de Satisfacción con los Estudios (EBSE) en estudiantes universitarios. Método: Se evaluó una muestra de 590 estudiantes (75,9% mujeres) de edades comprendidas entre los 17 y los 47 años (M = 20,37; DE = 3,155). Adicionalmente, se utilizaron medidas de ansiedad (GAD-2) y depresión (PHQ-2). Se aplicó un análisis factorial exploratorio, para conjuntamente diferenciar el EBSE, GAD-2 y PHQ-2. Finalmente, la fiabilidad por...

  15. A vida de Augusto, de Nicolau de Damasco: tradução acompanhada de breve introdução

    Directory of Open Access Journals (Sweden)

    Sandra Lúcia Rodrigues da Rocha

    2014-12-01

    Full Text Available A Vida de Augusto, de Nicolau de Damasco (séc. I a.C., é uma das mais antigas biografias de um rei na tradição helenística. Seu valor como fonte para o estudo do gênero bíos  impõe-se por si só. Neste trabalho, apresento uma breve introdução à primeira metade da obra em sua atual forma fragmentária, assim como uma tradução dos respectivos  fragmentos para o Português.

  16. Eficacia de la Terapia Psicoeducativa Motivacional Breve Dual (TPMB-D) en pacientes hospitalizados con trastorno por uso de sustancias y patología dual

    OpenAIRE

    Rubio Perlado, Begoña; Benito, Ana; Juan Porcar, María; Francés, Sonia; Real, Matías; Haro, Gonzalo

    2015-01-01

    Introducción: El término patología dual (PD) se refiere a la comorbilidad de los trastornos por uso de sustancias (TUS) con trastornos mentales graves. Se asocia con peores resultados de tratamiento que cada uno de estos trastornos por separado, por lo que es importante desarrollar terapias específicas para estos pacientes. En este sentido, el Hospital Provincial de Castellón adaptó la Terapia Psicoeducativa Motivacional Breve (efectiva en pacientes con TUS en Unidad de Desinto...

  17. Eficacia de la Terapia Psicoeducativa Motivacional Breve Dual (TPMB-D) en pacientes hospitalizados con trastorno por uso de sustancias y patología dual

    OpenAIRE

    Benito Delegido, Ana María

    2015-01-01

    El Hospital Provincial de Castellón adaptó la Terapia Psicoeducativa Motivacional Breve (efectiva en pacientes con trastorno por uso de sustancias (TUS) en Unidad de Desintoxicación Hospitalaria) para pacientes con patología dual (PD)(TPMB-D). Este estudio experimental doble ciego usó dos grupos aleatorios para evaluar la eficacia de la TPMB-D comparada con terapia ocupacional (TO) en pacientes hospitalizados con TUS y con PD. Completaron el estudio 163 pacientes, de los que el 68.7% prese...

  18. "Entre dos mundos" : las relaciones diplomáticas hispano-musulmanas durante la Edad Moderna : una breve síntesis

    Directory of Open Access Journals (Sweden)

    Miguel Fernando Gómez Vozmediano

    2008-01-01

    Full Text Available El presente artículo se ocupa, de forma breve y sintética, de una serie de contactos diplomáticos protagonizados durante la Edad Moderna por intermediarios (mercaderes, rescatadores de cautivos, renegados... que trabajaban al servicio de la Monarquía hispánica y de los gobiernos que conforman el actual Magreb.The present article briefly analyses a serie of diplomatic contacts carried out by intermediaries (merchants, redeemers of captives, renegades… serving the Spanish Monarchy and the North Africa governments all along the Early Modern Age.

  19. Psicoterapia psicodinâmica breve: estratégia terapêutica e mudança no padrão de relacionamento conflituoso

    OpenAIRE

    Yoshida,Elisa Medici Pizão; Elyseu Jr,Sebastião; Silva,Fernanda Robert de Carvalho Santos; Finotelli Jr,Itor; Sanches,Fabrícia Medeiros; Penteado,Elisa Frederich; Massei,Ariane Cristina; Rocha,Gláucia Mitsuko Ataka da; Enéas,Maria Leonor Espinosa

    2009-01-01

    Examinaram-se possíveis relações entre mudanças no padrão de relacionamento conflituoso de paciente, de 48 anos, submetida a psicoterapia breve psicodinâmica, e a estratégia terapêutica adotada pela terapeuta. Foi também avaliada a "magnitude" da mudança em sintomas psicopatológicos ao final do processo e entrevistas de acompanhamento (3 e 6 meses), com instrumentos de autorrelato: Inventário Beck de Depressão (BDI), Escala de Alexitimia de Toronto (TAS), Escala de Avaliação de Sintomas-40 (E...

  20. La legación de Francesco Barberini en España: unos retratos para el cardenal y un breve pontificio para Diego Velázquez, «clerico coniugato»

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    Giordano, Silvano

    2004-06-01

    Full Text Available New documents found in the Vatican Secret Archive concerning the shipment from Madrid to Rome of certain portraits painted by Velazquez for Cardinal Francesco Barberini are presented here. Among them is a preparatory drawing for a portrait of the Conde Duque de Olivares, to be painted according to the express indications left by the Cardinal and his secretary Cassiano del Pozzo in Madrid, in 1626. Also examined is the pontificial brief granted Velazquez in that year thanks to Barberini's intervention with the Pope, by which the painter could enjoy an ecclesiastical benefice even though he was a married layman with children. All of this information demonstrates the Conde Duque's interest in promoting the painter's career, the extraordinarily favorable treatment that the Sevillian received during his first years at the Alcázar, and his well-known slowness when it came time to paint, which —surprisingly— already manifested itself during this early period in the service of Felipe IV.

    Se presenta nueva documentación encontrada en el Archivo Secreto Vaticano sobre el envío —desde Madrid a Roma— de algunos retratos realizados por Velázquez para el cardenal Francisco Barberini. Entre éstos un dibujo preparatorio para una imagen del Conde Duque, que el sevillano tenía que pintar según expresas indicaciones dejadas por el cardenal y su secretario Cassiano dal Pozzo en Madrid, en 1626. Asimismo se estudia el breve pontificio concedido en ese año a Velázquez, gracias a la gestión de Barberini ante el Papa, y por el que el pintor pudo disfrutar de un beneficio eclesiástico aun siendo un laico casado y con hijos. Como veremos, todas estas noticias pondrán de manifiesto el interés del Conde Duque por la carrera del pintor, el extraordinario trato de favor que éste recibía durante sus primeros años en el Alcázar, así como su conocida flema a la hora de pintar, que ya se empieza a manifestar, sorprendentemente, desde sus primeros

  1. Psicoterapias breves psicodinâmicas: características da produção científica nacional e estrangeira (1980/2003 Brief psychodynamic psychotherapies: Characteristics of national and foreign scientific production (1980/2003

    Directory of Open Access Journals (Sweden)

    Elisa Medici Pizão Yoshida

    2005-06-01

    Full Text Available Apresenta o levantamento de algumas características formais da produção científica para saber quem, onde e o que tem sido indexado nas bases de dados eletrônicas, sob os verbetes: "psicoterapias breves psicodinâmicas" e "psicoterapias psicodinâmicas de tempo limitado". As produções estrangeira e nacional foram cotejadas e críticas apresentadas. A amostra de 534 resumos (1980 a 2003, publicados em bases nacionais e internacionais, indicou que a produção estrangeira foi quantitativamente superior quanto ao número de referências, periódicos científicos e autores. No entanto, a nacional equipara-se em aspectos tais como: média de referência por autor, predomínio de autoria única e adoção de psicoterapias integrativas, em que influências de outras abordagens, que não a psicodinâmica, são identificadas. Nas duas realidades predominaram, ainda, publicações sobre psicoterapias individuais de adultos, veiculadas por periódicos psiquiátricos. Espera-se que com a expansão das bases de dados da psicologia, esta última característica venha a se reverter, ao menos no Brasil.It presents a survey of some formal characteristics of the scientific production with the aim of knowing whom, where, and what have been indexed in electronic databases, under the entry: "brief psychodynamic psychotherapy" and "time-limited psychodynamic psychotherapy". Foreign and national productions were checked and critiques were presented. The sample of 534 abstracts (1980 to 2003, published in Brazil and abroad, indicated that the foreign production was quantitatively superior concerning the number of references, journals and authors. However, the national production compares in some aspects such as: reference average by author, predominance of unique authorship and adoption of integrative psychotherapy, in which influences of other approaches, rather than the psychodynamic, are identified. In the two realities there was a predominance of publications

  2. Jorge Santayana y sus vínculos humanos en Avila. Breve recopilación epistolar

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    Pedro GARCÍA MARTÍN

    2009-11-01

    Full Text Available Es, hasta cierto punto, conocida la vinculación abulense de Santayana. Se sabe que aquí pasó su infancia a partir de los dos años; se conocen también, en etapas posteriores, sus frecuentes escapadas veraniegas para ver a su padre primero y a sus hermanas después, especialmente a su hermana Susana que aquí casó y proporcionó finalmente al filósofo una verdadera familia hasta el final de sus días. Poco se ha profundizado, sin embargo, sobre el alcance real de esta influyente vinculación y sobre la cualidad particular de este sustrato abulense.

  3. Crônica política sobre um documento contra a "ditabranda"

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    Caio Navarro de Toledo

    2009-10-01

    Full Text Available Este breve artigo, uma crônica política, examina o significado e os efeitos políticos e ideológicos de um abaixo-assinado criado na Internet, em fevereiro de 2009. "Repúdio e Solidariedade" questionou a utilização do termo "ditabranda" - difundido pelo jornal paulista Folha de S. Paulo para designar a ditadura militar brasileira -, bem como prestou solidariedade a dois acadêmicos e intelectuais da Universidade de São Paulo (USP, conhecidos por suas atuações em defesa dos direitos humanos no Brasil. Subscrito por mais de 8 mil signatários, em pouco mais de seis semanas, o abaixo-assinado pode ser considerado - como testemunham os extensos comentários nele contidos - um relevante documento na luta pelo direito à verdade e à justiça sobre os fatos ocorridos durante o regime militar brasileiro (1964-1985. Talvez o seu papel simbólico mais relevante seja o de ter fincado uma bandeira na luta ideológica em torno da memória sobre 1964. No centro dessa bandeira seria reinscrita - como propôs um dos signatários do documento - a antiga consigna: no pasarán. Ou seja, os setores democráticos e progressistas da sociedade brasileira que apoiaram "Repúdio e Solidariedade" afirmam que não aceitarão calados as "falsificações da história" que impliquem o insulto à memória dos que lutaram, foram torturados e morreram na luta pela redemocratização do país.

  4. “Cara ou coroa”: uma provocação sobre educação para índios

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    Maria Helena Paes Rodrigues

    2003-01-01

    Full Text Available O artigo apresenta-se como espaço de reflexão e algumas provocações sobre o “fazer” em educação indígena, tendo como foco de discussão a atual configuração de mundo globalizado que, com o crescente uso da tecnologia, fragiliza fronteiras entre as mais diferentes comunidades. Neste sentido, apresenta uma breve discussão sobre a compreensão de cultura, sob a perspectiva do campo dos Estudos Culturais, destacando a constituição de culturas e identidades híbridas. Localizando a escola enquanto espaço e instituição legitimada de construção e transmissão de verdades e valores simbólicos, discute a função e importância desta para as comunidades indígenas. Assim, levantam-se alguns questionamentos sobre as “verdades” e “valores” dos modelos de escola necessária propostos pelos não-índios para as comunidades indígenas.

  5. Short Assessment of Health Literacy for Portuguese-speaking Adults Evaluación breve de alfabetismo en salud en portugués Avaliação breve de alfabetismo em saúde em português

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    Daniel Apolinario

    2012-08-01

    Full Text Available OBJECTIVE: To develop and validate a short health literacy assessment tool for Portuguese-speaking adults. METHODS: The Short Assessment of Health Literacy for Portuguese-speaking Adults is an assessment tool which consists of 50 items that assess an individual's ability to correctly pronounce and understand common medical terms. We evaluated the instrument's psychometric properties in a convenience sample of 226 Brazilian older adults. Construct validity was assessed by correlating the tool scores with years of schooling, self-reported literacy, and global cognitive functioning. Discrimination validity was assessed by testing the tool's accuracy in detecting inadequate health literacy, defined as failure to fully understand standard medical prescriptions. RESULTS: Moderate to high correlations were found in the assessment of construct validity (Spearman's coefficients ranging from 0.63 to 0.76. The instrument showed adequate internal consistency (Cronbach's alpha=0.93 and adequate test-retest reliability (intraclass correlation coefficient=0.95. The area under the receiver operating characteristic curve for detection of inadequate health literacy was 0.82. A version consisting of 18 items was tested and showed similar psychometric properties. CONCLUSIONS: The instrument developed showed good validity and reliability in a sample of Brazilian older adults. It can be used in research and clinical settings for screening inadequate health literacy.OBJETIVO: Desarrollar y validar un instrumento breve para evaluación de alfabetismo en salud en el idioma portugués. MÉTODOS: El instrumento desarrollado consiste de 50 itens que evalúan la capacidad del individuo de pronunciar y comprender términos médicos comunes. Las propiedades psicométricas se evaluaron en una muestra de 226 ancianos brasileños. La validez del constructo se estableció por la correlación con el número de años de escolaridad, relato de alfabetismo funcional y desempe

  6. Immunomodulatory Effects of Lactobacillus salivarius LS01 and Bifidobacterium breve BR03, Alone and in Combination, on Peripheral Blood Mononuclear Cells of Allergic Asthmatics.

    Science.gov (United States)

    Drago, Lorenzo; De Vecchi, Elena; Gabrieli, Arianna; De Grandi, Roberta; Toscano, Marco

    2015-07-01

    The aim of this study was to evaluate probiotic characteristics of Lactobacillus salivarius LS01 and Bifidobacterium breve BR03 alone and in combination and their immunomodulatory activity in asthmatic subjects. Subjects affected by allergic asthma were recruited. Initially, LS01 and BR03 were analyzed for their growth compatibility by a broth compatibility assay. To study the antimicrobial activity of probiotic strains, an agar diffusion assay was performed. Finally, cytokine production by peripheral blood mononuclear cells (PBMCs) stimulated with LS01 and BR03 was determined by means of specific quantitative enzyme-linked immunosorbent assay (ELISA). The growth of some clinical pathogens were slightly inhibited by LS01 and LS01-BR03 co-culture supernatant not neutralized to pH 6.5, while only the growth of E. coli and S. aureus was inhibited by the supernatant of LS01 and LS01-BR03 neutralized to pH 6.5. Furthermore, LS01 and BR03 combination was able to decrease the secretion of proinflammatory cytokines by PBMCs, leading to an intense increase in IL-10 production. L. salivarius LS01 and B. breve BR03 showed promising probiotic properties and beneficial immunomodulatory activity that are increased when the 2 strains are used in combination in the same formulation.

  7. Percepción del tiempo: resultados de una intervención grupal breve para el cambio del perfil temporal

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    Cristián Oyanadel

    2014-06-01

    Full Text Available La teoría de la perspectiva del tiempo de Zimbardo (1999 plantea una clasificación de las dimensiones de la orientación temporal, medidas con el Zimbardo Time Perspective Inventory (ZTPI, la cual propone, por una parte, que si se tiene una actitud positiva y moderada hacia el pasado, el presente y el futuro, se puede desarrollar mayor bienestar y buena salud tanto mental como física, mientras que actitudes negativas o extremas reflejarían pautas de vida poco saludables. El principal objetivo de esta investigación es evaluar el efecto de una intervención grupal breve para la modificación del perfil temporal. Para este fin, se realizó un estudio cuasiexperimental con grupo control en una muestra de 28 estudiantes universitarios; 14 de ellos formaron el grupo experimental y 14 formaron el grupo control. Los resultados muestran que la intervención realizada al grupo experimental modificó la orientación temporal de los participantes hacia un perfil equilibrado. Para terminar, se concluye que, con una intervención breve y grupal que potencie un pasado positivo y un futuro y un presente más saludables, es posible lograr un perfil equilibrado. Posteriores estudios podrían evaluar el impacto de la modificación del perfil temporal en población clínica.

  8. Percepción del tiempo: resultados de una intervención grupal breve para el cambio del perfil temporal

    Directory of Open Access Journals (Sweden)

    Cristián Oyanadel

    2014-06-01

    Full Text Available La teoría de la perspectiva del tiempo de Zimbardo (1999 plantea una clasificación de las dimensiones de la orientación temporal, medidas con el Zimbardo Time Perspective Inventory (ZTPI, la cual propone, por una parte, que si se tiene una actitud positiva y moderada hacia el pasado, el presente y el futuro, se puede desarrollar mayor bienestar y buena salud tanto mental como física, mientras que actitudes negativas o extremas reflejarían pautas de vida poco saludables. El principal objetivo de esta investigación es evaluar el efecto de una intervención grupal breve para la modificación del perfil temporal. Para este fin, se realizó un estudio cuasiexperimental con grupo control en una muestra de 28 estudiantes universitarios; 14 de ellos formaron el grupo experimental y 14 formaron el grupo control. Los resultados muestran que la intervención realizada al grupo experimental modificó la orientación temporal de los participantes hacia un perfil equilibrado. Para terminar, se concluye que, con una intervención breve y grupal que potencie un pasado positivo y un futuro y un presente más saludables, es posible lograr un perfil equilibrado. Posteriores estudios podrían evaluar el impacto de la modificación del perfil temporal en población clínica.

  9. IMPACTO DEL TRATADO DE LIBRE COMERCIO ENTRE COLOMBIA Y EEUU, SOBRE EL ACCESO A LOS MEDICAMENTOS EN COLOMBIA

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    José David Millán Cano

    2012-12-01

    Full Text Available Este artículo, más que pretender continuar con la franca crítica al Tratado de Libre Comercio (TLC entre Colombia y EE.UU, busca dar una aproximación a las posibles repercusiones que este acuerdo tendrá sobre el acceso a los medicamentos de los afiliados al Sistema General de Seguridad Social en Salud (SGSSS colombiano en  mediano y largo plazo.Para lograr esto, se definirá qué se entiende por Tratado de Libre Comercio, para luego realizar una breve reseña histórica de su evolución. Luego, y para entender un poco la relación que ha unido a Colombia y a EEUU en términos comerciales, se intentará dar un panorama histórico sobre el tema. Se continuará con una descripción de cómo fue el proceso de negociación sin perder de vista los antecedentes y causas de tipo político, económico, y social que motivaron un TLC entre Colombia y EEUU, teniendo en cuenta qué sectores quedaron incluidos en la negociación, qué servicios y con qué condiciones, para luego, evaluar cómo se ve afectado el derecho a la salud y sobre todo el acceso a los medicamentos para los afiliados al SGSSS colombiano a mediano y largo plazo.

  10. Primera consulta sobre la industria de los materiales de la construcción organizada por ONUDI y UNCHS (HABITAT

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    Salas, Julián

    1985-06-01

    Full Text Available This work, devoted to the First Consultation sponsored by ONUDI-UNCHS (HABITAT relative to "Building Materials Industry", selects three different sides of it: the final version of the conclusions and recommendations prepared in Athens by the participants, the author personal answers, as a participant himself, to some questions elabored by the Consultation organizers as well as a brief account of the round tables held in the IETcc with some of the Latin American professionals who took part in the X Course of High Studies on Construction focused on this important event. Thus the aim is to try to spread among the readers of spanish language the Athens recommendations on the industry development of building materials in developing countries.

    Se recogen en este trabajo, sobre la Primera Consulta patrocinada por ONUDI-UNCHS (HABITAT relativa a la "Industria de los materiales de construcción", tres aspectos bien distintos sobre la misma: la versión definitiva de las conclusiones y recomendaciones elaboradas por los participantes en Atenas; las respuestas personales del autor, como participante, a algunas de las preguntas preparadas por los organizadores de la Consulta, así como una breve reseña de las mesas redondas celebradas en el lETcc con algunos de los profesionales iberoamericanos participantes en el X Curso de Estudios Mayores de la Construcción centradas en este importante evento. El objetivo, por tanto, es el de tratar de divulgar entre los lectores de habla hispana las recomendaciones de Atenas sobre el desarrollo de la industria de los materiales de construcción en los países en vías de desarrollo.

  11. Uma breve história do ópio e dos opióides Una historia breve del opio y de los opioides Opium and opioids: a brief history

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    Danilo Freire Duarte

    2005-02-01

    Full Text Available JUSTIFICATIVA E OBJETIVOS: Desde tempos imemoriais, o ópio e os seus derivados, além de exercerem ponderável influência sobre o comportamento dos seres humanos, têm sido empregados como sedativo e como analgésico. A partir do século XIX, com o isolamento dos alcalóides do ópio e as facilidades para o emprego dessas substâncias por via parenteral, houve aumento do interesse pelo uso criterioso dos opióides na área médica e da análise das conseqüências sociais de seu uso abusivo. Justifica-se, pelo exposto, uma revisão histórica do ópio e dos seus derivados. CONTEÚDO: A evolução dos conhecimentos sobre o ópio, produto natural extraído do Papaver somniferum, e sobre os opióides, substâncias naturais, semi-sintéticas e sintéticas extraídas do ópio, bem como as principais referências a essas substâncias desde a Antigüidade foram avaliadas. Foi enfatizado o progresso obtido a partir dos trabalhos de Setürner que resultaram no isolamento da morfina. As investigações conduzidas por outros autores na busca de substâncias sintéticas que apresentassem vantagens sobre os produtos naturais foram mencionadas. A importância da descoberta dos receptores opióides e de seus ligantes endógenos foi sublinhada. CONCLUSÕES: No alvorecer do terceiro milênio, a despeito das pesquisas realizadas com drogas analgésicas de outros grupos farmacológicos, os opióides continuam sendo os analgésicos mais potentes, embora sua eficácia seja contestada em certos tipos de dor. Os atuais conhecimentos de Farmacologia Clínica permitem selecionar o opióide a ser administrado, considerando a doença e as condições do paciente, na busca da melhor relação custo-benefício.JUSTIFICATIVA Y OBJETIVOS: Desde tiempos inmemoriales, el opio y sus derivados, junto con ejerceren ponderable influencia sobre el comportamiento de los seres humanos, han sido empleados como sedante y como analgésico. Desde el siglo XIX, con el aislamiento de los

  12. Sobre aprendizaje escolar y neurociencias

    OpenAIRE

    Terigi, Flavia

    2016-01-01

    En el marco de una creciente difusión de los discursos de y sobre las neurociencias en el campo educativo en Argentina, donde enunciados extraídos del discurso neurocientífico comienzan a transformarse en emblemas de política pública, este trabajo, escrito desde el punto de vista de la educación escolar, se propone situar los alcances y límites de los aportes de estas disciplinas para comprender el aprendizaje escolar. En él se postula que la aparición rutilante de las neurociencias en educac...

  13. Polinomios perfectos sobre F2

    OpenAIRE

    Cely Rojas, Valeria

    2010-01-01

    Este trabajo muestra el status de los números perfectos y el análogo de los números perfectos en los anillos de polinomios. Además, se presentan las demostraciones de algunas propiedades de los polinomios perfectos sobre F2 de forma comprensible, basadas en el trabajo realizado por los profesores Luis Gallardo y Oliver Rahavandrainy. / Abstract. This work shows the status artis of perfect numbers and their analogue in the rings of polynomials. Proofs of some properties of perfect polynomials ...

  14. Contabilidad del impuesto sobre beneficios

    OpenAIRE

    Luceño Martínez, Haizea

    2017-01-01

    Este Trabajo Fin de Grado pretende dar una explicación al tratamiento contable del Impuesto de Sociedades. Siendo un impuesto que hay que contabilizar, pero que se calcula siguiendo criterios fiscales, lleva a tener que realizar ajustes que tendrán su repercusión contable. El punto de partida no es otra que la norma de registro y valoración 13ª del PGC, que versa sobre cómo hay que proceder a contabilizar el Gasto por Impuesto de Sociedades, que se compone tanto del Gasto por Impuesto Co...

  15. Apontamento sobre a contabilidade em Portugal entre 1900 e 1950

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    Hernâni Olímpio Carqueja

    2011-07-01

    Para esclarecer el contexto en 1900 se procede a una breve revisión de los antecedentes desde la creación del “Aula do Comércio de Lisboa”, que es el referente de la enseñanza oficial organizada, incluyendo la partida doble, hasta 1900.

  16. Necessidade de intervenção breve por uso de álcool, tabaco e outras drogas entre usuários da atenção primária à saúde

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    Ângela Maria Mendes Abreu

    Full Text Available RESUMO Objetivos Rastrear o uso de álcool, tabaco e outras drogas entre usuários da atenção primária à saúde e identificar fatores associados à necessidade de intervenção breve por uso dessas substâncias. Métodos Estudo transversal desenvolvido com 1.489 usuários de unidade básica de saúde da cidade do Rio de Janeiro, Brasil, que responderam ao Alcohol, Smoking and Substance Involvement Screening Test entre os anos 2013 e 2014. Regressão logística multivariada foi utilizada para identificar características sociodemográficas associadas à necessidade de intervenção breve por uso de álcool e outras drogas. Resultados As substâncias mais usadas foram álcool (39,5%, tabaco (20,3% e, dentre as drogas ilícitas, maconha (1,9%. Em ambos os sexos, maiores chances de necessidade de intervenção breve por uso de tabaco e álcool foram encontradas entre pessoas de baixa escolaridade, renda domiciliar alta e que não possuíam religião. Conclusão Os resultados apontam características socioeconômicas como determinantes de necessidade de intervenção breve por uso de álcool e tabaco.

  17. Estudo da efetividade da intervenção breve para o uso de álcool e outras drogas em adolescentes atendidos num serviço de assistência primária à saúde Study on the effectiveness of brief intervention for alcohol and other drug use directed to adolescents in a primary health care unit

    Directory of Open Access Journals (Sweden)

    Denise De Micheli

    2004-09-01

    Full Text Available OBJETIVOS: O principal objetivo deste estudo foi avaliar a efetividade de uma intervenção breve e de uma orientação preventiva do uso de álcool e/ou outras drogas, dirigidas a adolescentes. MÉTODOS: Noventa e nove adolescentes que buscaram atendimento médico em um serviço ambulatorial especializado no atendimento de adolescentes foram divididos de acordo com seus níveis de consumo de substâncias em usuários no último mês (UM e não usuários no último mês (NUM. Cada um destes grupos foi dividido em dois: um grupo controle de usuários no último mês (COUM, um grupo controle de não usuários no último mês (CONUM, um grupo que receberia Intervenção Breve no caso de serem usuários no mês (UM-IB e um grupo que receberia Orientação Preventiva no caso de serem não usuários no mês (NUM-OP, totalizando quatro grupos. A orientação preventiva teve duração de 2 a 3 minutos e intervenção breve de 20 minutos, sendo realizada segundo roteiro pré-estruturado. Todos os participantes foram novamente avaliados após seis meses. RESULTADOS: No seguimento realizado ao final de seis meses, observou-se um aumento significativo na prevalência de consumo de maconha, álcool e tabaco bem como na intensidade de problemas e comportamentos de risco no grupo CONUM. No grupo NUM-OP, embora também tenha sido observado aumento na prevalência de consumo de drogas lícitas (álcool e tabaco, este foi significativamente menor em freqüência e intensidade do que o observado no grupo CONUM. Além disso, neste grupo não ocorreu aumento no uso de maconha. No grupo de adolescentes usuários no último mês que receberam Intervenção Breve (UM-IB, observou-se redução significativa na proporção de usuários em relação à maioria das substâncias avaliadas, bem como redução na intensidade dos problemas e comportamentos de risco. CONCLUSÃO: Nossos resultados confirmam a efetividade de uma sessão única de intervenção breve dirigida a

  18. O ensino de línguas para crianças no contexto educacional brasileiro: breves reflexões e possíveis provisões The teaching of children in the Brazilian educational context: brief reflections and possible provisions

    Directory of Open Access Journals (Sweden)

    Cláudia Hilsdorf Rocha

    2007-01-01

    Full Text Available O objetivo deste artigo é discutir acerca do ensino de línguas estrangeiras (LE para crianças. Respaldando-nos em princípios da teoria Sócio-cultural (Vygotsky, 1978 e do Comunicativismo (Almeida Filho, 1993, 2005, voltados para o ensino de línguas (inglês para crianças (Cameron, 2001; Brewster, Ellis & Girard, 2002; entre outros, teceremos reflexões sobre a importância da aprendizagem de línguas na infância, sobre a relevância motivação e da qualidade das interações para um ensino cujo objetivo principal recai na formação integral do aluno, como também sobre o papel da gramática, da oralidade e da língua materna no processo. Serão também feitas considerações acerca da importância da formação (contínua do professor atuante nesse contexto e de possíveis procedimentos no ensino em pauta, quando serão apresentadas breves proposições orientadas pela noção bakhtiniana de gêneros.This article aims at discussing about the foreign language teaching to young learners, taking the principles of the Sociocultural Theory (Vygotksy, 1978 and of the Communicative Approach (Almeida Filho, 1993, 2005 related to Primary English teaching (Cameron, 2001; Brewster, Ellis & Girard, 2002 as a theoretical references. Considerations about the importance of language learning in childhood will be made, as well as about the role of the grammar, oral language and mother tongue in the process. Likewise, the importance of Teacher Education will be briefly approached. This work is ended with the discussion about some possible procedures in the language teaching processes followed by a brief presentation of possible guidelines based on the bakhtinian notion of discourse genres.

  19. Constituição cidadã e representações sociais: uma reflexão sobre modelos de assistência à saúde Constitución ciudadana y representaciones sociales: una reflexión sobre modelos de atención de la salud Citizen constitution and social representations: reflecting about health care models

    Directory of Open Access Journals (Sweden)

    Sílvio Éder Dias da Silva

    2010-12-01

    Full Text Available Este artigo apresenta uma reflexão sobre o significado dos termos cidadania e saúde, abordando a Teoria das Representações Sociais como estratégia para implementação e avaliação dos modelos de assistência a saúde no Brasil. Na primeira parte, traçamos um breve histórico sobre a concepção de cidadania; na segunda, tratamos dos princípios de liberdade e igualdade pautados no pensamento de Kant; na terceira, evidenciamos a saúde como um direito do cidadão e um dever do estado; por fim, destacamos a Teoria das Representações Sociais como estratégia para avaliar e implementar os serviços de saúde prestados ao cidadão pelos modelos assistenciais de saúde em vigor no Brasil.Este artículo expone una reflexión sobre el significado del término ciudadanía, salud y la Teoría de las Representaciones Sociales como una estrategia para la implementación y evaluación de los modelos de atención a la salud en Brasil. En la primera parte, trazamos un breve resumen histórico sobre la concepción de ciudadanía; en la segunda parte, abordamos los principios de libertad e igualdad pautados en el pensamiento de Kant; en la tercera, dejamos evidenciado que la salud es un derecho del ciudadano y un deber del estado y, por fin, destacamos la Teoría de las Representaciones Sociales como una estrategia para evaluar e implementar los servicios de salud prestados al ciudadano por los modelos de atención a la salud vigentes en Brasil.This article presents a reflection on the meaning of the terms citizenship and health, addressing the Theory of Social Representations as a strategy for implementing and evaluating health care models in Brazil. First, a brief history about the concept of citizenship is presented; then the article addresses the principles of freedom and equality according to Kant; the third section of the article shows that health is as a right of the citizen and a duty of the state. Finally, the Theory of Social Representations

  20. Recursos y enlaces sobre comunidades de aprendizaje

    OpenAIRE

    Ferrer Esteban, Gerard

    2005-01-01

    Obras y artículos sobre el proyecto Comunidades de Aprendizaje desarrollado en el Estado español. Tesis doctorales sobre comunidades de aprendizaje. Fundamentación teórica de las comunidades de aprendizaje. Sitios en Internet relacionados con las comunidades de aprendizaje y proyectos afines de desarrollo comunitario en contextos escolares.

  1. Um Breve Panorama das Relações entre Política e Religião no Contexto do Estado Moderno

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    Marco Aurélio Lagreca Casamasso

    2010-12-01

    Full Text Available O artigo objetiva proporcionar, a partir da perspectiva do pensamento político moderno, um breve panorama do modelo de relação que se estabelece entre a política e a religião no contexto do Estado Moderno. Destaca-se a hegemonia do poder estatal no âmbito da sociedade política e, em particular, sua supremacia em face da religião e dos atores religiosos. Apresenta-se um relato sucinto da problemática político-religiosa no pensamento de Maquiavel, Hobbes, Spinoza, Locke, Montesquieu e Rousseau.

  2. Breves consideraciones acerca de la importancia de los árboles de decisión en el análisis de carteras

    OpenAIRE

    Julio César Riascos Hermoza; Jesús Enrique Molina Muñoz

    2016-01-01

    La cada vez más estrecha relación entre las ciencias y/o disciplinas no es ajena a la relación derivada en los últimos 50 años entre las nanzas, la Econometría, la modelación estadística y los procesos de simulación computacional. El propósito de este artículo introductorio reside en exponer algunas breves consideraciones con respecto al potencial manejo de los árboles de decisión en la teoría nanciera y particularmente como complemento al diseño de los tradicionales árboles de rentabilidad. ...

  3. Mini International Neuropsychiatric Interview (MINI): validação de entrevista breve para diagnóstico de transtornos mentais

    OpenAIRE

    Amorim, Patrícia

    2000-01-01

    Objetivos: O MINI é uma entrevista diagnóstica padronizada breve (15-30 minutos), compatível com os critérios do DSM-III-R/IV e da CID-10, que é destinada à utilização na prática clínica e na pesquisa em atenção primária e em psiquiatria, e pode ser utilizada por clínicos após um treinamento rápido (de 1 a 3 horas). A versão Plus do MINI, mais detalhada, gera diagnósticos positivos dos principais transtornos psicóticos e do humor do DSM-IV. Este artigo apresenta os resultados de quatro estudo...

  4. Efecto cristal. Reflexiones sobre arquitectura

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    Dr. Arq. Carlos Pantaleón Panaro

    2017-12-01

    Full Text Available ESPEJISMO es una ilusión óptica debido a la reflexión total de la luz cuando atraviesa capas de aire de densidad distinta, con lo cual los objetos lejanos dan una imagen invertida, como si se reflejaran sobre el agua, tal como ocurre en las llanuras de los desiertos. 1 La definición de espejismo nos explica que es una ilusión, una ficción, un desvarío, un delirio. Algo que no es real ni verdadero, la apariencia engañosa de algo que no existe. ¿Pero no existe, realmente? Tal vez se pueda decir, desde algún punto de vista, que una imagen ilusoria no es real. Pero ¿podríamos afirmarlo desde el punto de vista del fenómeno de la percepción? Si lo vemos, ¿no tendríamos derecho a decir que es real? ¿Algo tiene que ser material y poder tocarse, para ser real? ¿No basta simplemente con verlo? Este artículo de reflexión trata sobre el tema de lo ilusorio, de los espejismos y apariencias engañosas que, no obstante su naturaleza falaz, existen y se perciben, y son procesados por nuestra mente como fenómenos sensoriales que conforman nuestra percepción. El hilo conductor es un viaje a través de diferentes paisajes espejados: el del azar, el de las artes y el de la arquitectura. 1Diccionario de la Real Academia Española

  5. DE MALINOWSKI AOS PÓS-MODERNOS: UMA BREVE REFLEXÃO ACERCA DA PESQUISA ETNOGRÁFICA NA ANTROPOLOGIA

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    Vanessa Silva dos Santos

    2017-02-01

    Full Text Available O presente ensaio busca refletir acerca do método etnográfico ela- borado por Bronislaw Malinowski (1976 [1922], enfatizando as contribuições do autor, sem perder de vista as dinâmicas que vêm ocorrendo no tipo de análise por ele inaugurada. Esse texto resulta de uma revisão bibliográfica, ao revisitarmos alguns dos vários autores que vêm se debruçando analiticamente sobre o método etnográfico e a pesquisa de campo. Dessa forma, defendemos as contribuições metodológicas sistematizadas por Malinowski, sem perder de vista as críticas contemporâneas acerca dos limites dessa obra.

  6. Los cambios en la familia vistos desde la demografía; una breve reflexión

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    Julieta Quilodrán

    2008-01-01

    Full Text Available Este texto contiene una serie de reflexiones producto de años de investigación y docencia en torno a los temas de fecundidad y nupcialidad, entendida esta última en su acepción más amplia de proceso de formación familiar. En primer lugar busca ubicar la evolución de estos dos fenómenos en el contexto de la dinámica demográfica general para luego destacar cuáles son los principales cambios observados en las sociedades desarrolladas y en la región latinoamericana. La transición demográfica que se efectuó durante dos si-glos en los países de Europa, está realizándose en menos de uno en los que la iniciaron entrado el siglo xx. En torno a estos fenómenos -nupcialidad y fecundidad- han ocu-rrido al menos dos hechos inesperados: el descenso de la fecundidad por debajo del nivel de reemplazo y las transformaciones en la formación y la estabilidad de las parejas con-yugales. En el ámbito de las familias, junto con el descenso continuo de la fecundidad de las parejas se ha incrementado la sexualidad fuera del matrimonio, los hijos nacidos al margen de éste, el control natal, la interrupción de uniones, las nuevas nupcias y las uniones informales.El objetivo del artículo es analizar los aspectos de la nupcialidad que están ejerciendo influencia sobre el régimen reproductivo en la era postransicional y que son justamente los que están en el centro de la discusión sobre la segunda transición demográfica.

  7. Probiotic Streptococcus thermophilus FP4 and Bifidobacterium breve BR03 Supplementation Attenuates Performance and Range-of-Motion Decrements Following Muscle Damaging Exercise

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    Ralf Jäger

    2016-10-01

    Full Text Available Probiotics have immunomodulatory effects. However, little is known about the potential benefit of probiotics on the inflammation subsequent to strenuous exercise. In a double-blind, randomized, placebo controlled, crossover design separated by a 21-day washout, 15 healthy resistance-trained men ingested an encapsulated probiotic Streptococcus (S. thermophilus FP4 and Bifidobacterium (B. breve BR03 at 5 bn live cells (AFU concentration each, or a placebo, daily for 3 weeks prior to muscle-damaging exercise (ClinicalTrials.gov NCT02520583. Isometric strength, muscle soreness, range of motion and girth, and blood interleukin-6 (IL-6 and creatine kinase (CK concentrations were measured from pre- to 72 h post-exercise. Statistical analysis was via mixed models and magnitude-based inference to the standardized difference. Probiotic supplementation resulted in an overall decrease in circulating IL-6, which was sustained to 48 h post-exercise. In addition, probiotic supplementation likely enhanced isometric average peak torque production at 24 to 72 h into the recovery period following exercise (probiotic–placebo point effect ±90% CI: 24 h, 11% ± 7%; 48 h, 12% ± 18%; 72 h, 8% ± 8%. Probiotics also likely moderately increased resting arm angle at 24 h (2.4% ± 2.0% and 48 h (1.9% ± 1.9% following exercise, but effects on soreness and flexed arm angle and CK were unclear. These data suggest that dietary supplementation with probiotic strains S. thermophilus FP4 and B. breve BR03 attenuates performance decrements and muscle tension in the days following muscle-damaging exercise.

  8. A synbiotic mixture of scGOS/lcFOS and Bifidobacterium breve M-16V increases faecal Bifidobacterium in healthy young children.

    Science.gov (United States)

    Kosuwon, P; Lao-Araya, M; Uthaisangsook, S; Lay, C; Bindels, J; Knol, J; Chatchatee, P

    2018-04-10

    Little is known about the impact of nutrition on toddler gut microbiota. The plasticity of the toddler gut microbiota indicates that nutritional modulation beyond infancy could potentially impact its maturation. The objective of this study was to determine the effect of consuming Young Child Formula (YCF) supplemented with short chain galactooligosaccharides and long chain fructooligosaccharides (scGOS/lcFOS, ratio 9:1) and Bifidobacterium breve M-16V on the development of the faecal microbiota in healthy toddlers. A cohort of 129 Thai children aged 1-3 years were included in a randomised controlled clinical study. The children were assigned to receive either YCF with 0.95 g/100 ml of scGOS/lcFOS and 1.8×10 7 cfu/g of B. breve M-16V (Active-YCF) or Control-YCF for 12 weeks. The composition and metabolic activity of the faecal microbiota, and the level of secretory immunoglobulin A were determined in the stool samples. The consumption of Active-YCF increased the proportion of Bifidobacterium (mean 27.3% at baseline to 33.3%, at week 12, P=0.012) with a difference in change from baseline at week 12 between the Active and Control of 7.48% (P=0.030). The consumption of Active-YCF was accompanied with a more acidic intestinal milieu compared to the Control-YCF. The pH value decreased statistically significantly in the Active-YCF group from a median of 7.05 at baseline to 6.79 at week 12 (Pbreve M-16V positively influences the development of the faecal microbiota in healthy toddlers by supporting higher levels of Bifidobacterium. The synbiotic supplementation is also accompanied with a more acidic intestinal milieu and softer stools.

  9. Probiotic Streptococcus thermophilus FP4 and Bifidobacterium breve BR03 Supplementation Attenuates Performance and Range-of-Motion Decrements Following Muscle Damaging Exercise.

    Science.gov (United States)

    Jäger, Ralf; Purpura, Martin; Stone, Jason D; Turner, Stephanie M; Anzalone, Anthony J; Eimerbrink, Micah J; Pane, Marco; Amoruso, Angela; Rowlands, David S; Oliver, Jonathan M

    2016-10-14

    Probiotics have immunomodulatory effects. However, little is known about the potential benefit of probiotics on the inflammation subsequent to strenuous exercise. In a double-blind, randomized, placebo controlled, crossover design separated by a 21-day washout, 15 healthy resistance-trained men ingested an encapsulated probiotic Streptococcus ( S. ) thermophilus FP4 and Bifidobacterium ( B. ) breve BR03 at 5 bn live cells (AFU) concentration each, or a placebo, daily for 3 weeks prior to muscle-damaging exercise (ClinicalTrials.gov NCT02520583). Isometric strength, muscle soreness, range of motion and girth, and blood interleukin-6 (IL-6) and creatine kinase (CK) concentrations were measured from pre- to 72 h post-exercise. Statistical analysis was via mixed models and magnitude-based inference to the standardized difference. Probiotic supplementation resulted in an overall decrease in circulating IL-6, which was sustained to 48 h post-exercise. In addition, probiotic supplementation likely enhanced isometric average peak torque production at 24 to 72 h into the recovery period following exercise (probiotic-placebo point effect ±90% CI: 24 h, 11% ± 7%; 48 h, 12% ± 18%; 72 h, 8% ± 8%). Probiotics also likely moderately increased resting arm angle at 24 h (2.4% ± 2.0%) and 48 h (1.9% ± 1.9%) following exercise, but effects on soreness and flexed arm angle and CK were unclear. These data suggest that dietary supplementation with probiotic strains S. thermophilus FP4 and B. breve BR03 attenuates performance decrements and muscle tension in the days following muscle-damaging exercise.

  10. A transferência nos atendimentos breves de orientação psicanalítica/The short on transfer of oriented psychotherapy

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    Gabriela Gomes Costardi

    2006-01-01

    Full Text Available Esse trabalho tem como objetivo discutir a especificidade da transferência em psicoterapia breve. A metodologia utilizada é um estudo de caso. A relação de reciprocidade estabelecida inicialmente pela dupla terapêutica demarcou o espaço psicoterápico como um lugar de ‘palavra vazia’ e o cliente só pôde falar do que lhe fazia sofrer quando a estagiária saiu do lugar de sujeito que ocupava na relação. Esse deslocamento teve um efeito terapêutico para o cliente, que se fez marcar pela subtração da palavra. "Quem fala perde"(sic. Perder este lugar-sintoma foi o efeito resultante deste tratamento. Conclui-se que a proposta dos atendimentos breves de orientação psicanalítica opera a partir da sustentação do cliente enquanto sujeito do tratamento. This paper aims to discuss the specificity of transference in short-term psychotherapy. The methodology used is a case study. The reciprocity relationship established at first by the therapeutic pair determined the psychotherapeutic space as a place for an “empty word”; the patient was only able to talk about the source of his suffering when the intern stepped out of her role as the subject she had been playing in the relationship. That move had a therapeutic effect on the patient, marked by the subtraction of the word - “the one who speaks loses”. Losing that place/symptom was the resulting effect of the treatment. The conclusion herein is that the proposal of short-term psychoanalytical treatments operates from the establishment of the patient as the subject in the treatment.

  11. Transferência erótica: uma breve revisão Transferencia erótica: una breve revisión Erotic transference: a brief review

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    Luciano Rassier Isolan

    2005-08-01

    Full Text Available A transferência erótica é um processo relativamente comum, tanto na prática de psicoterapia de orientação analítica quanto de psicanálise. Apesar de, muitas vezes, trazer dificuldades no seu manejo, trata-se de um fenômeno que, se adequadamente compreendido e manejado, pode se transformar em uma ferramenta útil para o processo terapêutico. Neste trabalho será realizada uma revisão sobre o conceito de transferência erótica, ressaltando-se as suas dificuldades de manejo técnico, bem como o uso da contratransferência e a influência do gênero nessa situação.La transferencia erótica es un proceso relativamente común, tanto en la práctica de psicoterapia de orientación analítica como de psicoanálisis. A pesar de, muchas veces, traer dificultades en su manejo, se trata de un fenómeno que, si adecuadamente comprendido y manejado, puede transformarse en una herramienta útil al proceso terapéutico. En este trabajo, será realizada una revisión sobre el concepto de transferencia erótica, resaltándose sus dificultades de manejo técnico, así como el uso de la contratransferencia y la influencia del género en esa situación.Erotic transference is a relatively common process in psychotherapy and psychoanalysis. In spite of its difficult management, when appropriately understood and managed, erotic transference may become a useful tool in the therapeutic process. In this review, we will address the concept of erotic transference, with emphasis on the difficulties faced in the technical management of the process, as well as the use of countertransference and the influence of gender in this situation.

  12. Puente sobre el rio Medway

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    Freeman, Fox, Ingenieros

    1963-02-01

    have been prestressed both longitudinally and transversally. Between the two circulation roadways an elastic barrier has been placed, supported on metal posts.El puente sobre el río Medway tiene la misión de salvar dicho río en el nuevo trazado de carretera necesario para aliviar el denso tráfico estival entre Dower y Londres. Está situado en las proximidades de la ciudad de Rochester. La estructura se ha construido utilizando las vías de concurso. De todos los tipos presentados se eligió una estructura de hormigón armado. La longitud total de la obra es de 1.000 m. Los tres tramos principales de la obra salvan el río. El central es de 152 m de luz, y los dos adyacentes son de 103 m cada uno. Los accesos tienen un total de 18 tramos, que arrojan una longitud de 654 m. La anchura del puente es de 34 m, y la calzada se ha subdividido en dos direcciones de tráfico de 7,30 m cada una y, además, se han dejado dos espaldones laterales de 2,40 m de anchura. En planta se puede admitir que el puente tiene una alineación recta con una ligerísima curva, y que en el plano vertical presenta una pendiente de desagüe entre las pilas 9 y 10. Los estribos son de hormigón armado: tipo celular el del este y de hormigón en masa el del oeste. Los estribos se apoyan sobre pilotes hexagonales de hormigón armado cuya circunferencia circunscrita tiene 55 cm de radio y de 12 a 20 m de longitud. Cuando el firme se hallaba a profundidades inferiores a 6 m, los estribos se cimentaban directamente sobre el firme. Las pilas constituyen pórtico y tienen una altura variable de 9 a 30 m sobre el nivel del suelo. Los tramos de acceso forman una estructura continua simplemente apoyada. Cada tramo se compone de ocho vigas longitudinales de 30 a 41 m de longitud y de 190 toneladas cada una. Estas vigas son del tipo pretensado y prefabricado. El tramo central es del tipo cantilever, constituido por dos brazos, de 30 m de longitud, formados por vigas cajón. Cada una de estas vigas consta

  13. El pensamiento económico de Juan de Lugo. Un estudio sobre sus teorías del precio justo, del dinero y del interés

    OpenAIRE

    Monsalve Serrano, Favio

    2002-01-01

    Esta tesis aborda el pensamiento económico de Juan de Lugo y, más concretamente, sus teorías del precio justo, del dinero y de la usura. También aborda un breve análisis comparativo entre Juan de Lugo y escolásticos coetáneos, principalmente Luis de Molina, así como una proyección de las ideas de Lugo sobre el análisis económico moderno. La teoría del precio justo se aborda desde un enfoque analítico mostrando cada uno de los elementos que componen dicha teoría (respeto a la justicia, teor...

  14. Escuela e inmigración: una presentación de estudios sobre el desarrollo de habilidades lingüísticas y procesos de interdependencia entre lenguas

    Directory of Open Access Journals (Sweden)

    Ángel Huguet

    2013-05-01

    Full Text Available Al objeto de profundizar en el debate "inmigración-lengua-escuela", el trabajo presenta algunos datos derivados de la todavía escasa investigación sobre el tópico existente hasta el momento en España, poniendo especial énfasis en el caso catalán. Para ello, a partir de una breve revisión de la incidencia demográfica del fenómeno migratorio en el conjunto del Estado, mostramos los resultados de una serie de estudios centrados en: 1 el desarrollo de habilidades lingüísticas en la lengua de la escuela, y 2 el análisis de los procesos de interdependencia lingüística. Finalmente, se discuten posibles actuaciones que persiguen optimizar y reconsiderar la práctica educativa en las aulas multilingües.

  15. LOS PROGRAMAS DE CUALIFICACIÓN PROFESIONAL INICIAL: UNA VISIÓN PANORÁMICA SOBRE LAS MODALIDADES Y PERFILES PROFESIONALES EN LA REGIÓN DE MURCIA

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    Mª Teresa González González

    2012-01-01

    Full Text Available A lo largo de este artículo se abordan algunas cuestiones relacionadas con el Programa de Cualificación Profesional Inicial (PCPI. En el primer y segundo apartado del artículo se realiza una breve caracterización de este programa destinado a alumnos en situación de riesgo de exclusión educativa, así como del escenario educativo en el que se plantea. En el tercero se ofrecen datos sobre los perfiles profesionales de los PCPI que se están desarrollando en la Comunidad de la Región de Murcia (CARM. Tales datos derivan de una investigación más amplia sobre este programa y fueroobtenidos de la página web de la Consejería de Educación, y analizados una vez que se ordenaron y agruparon según modalidades de PCPI, perfiles profesionales, y centros educativos ayuntamientos u otras entidades que los imparten.El análisis realizado pone de manifiesto que no existe un patrón que caracterice la distribución de PCPI según perfiles profesionales. Éstos no se circunscriben a una u otra modalidad de PCPI como tampoco quedan ligados a la actividad económica y empresarial de las zonas en la que se...

  16. Una noticia desconcertante sobre la inscripción de San Juan de Baños ofrecida por Alvar Gómez de Castro

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    Velázquez, Isabel

    2000-12-01

    Full Text Available The paper presents and analyses brief unpublished notes by the humanist Alvar Gómez de Castro, conserved in MS number 7896 in the Biblioteca Nacional, regarding five known inscriptions. Their interest lies in the fact that they are the first references to each one and provide useful data on the history of the pieces. But the most striking is the presentation of an inscription in S. Román de Hornija, the text of which is identical to the inscription of S. Juan de Baños. The paper presents hypotheses on this puzzling and perhaps erroneous fact.Se realiza la presentación y análisis de unas breves notas inéditas del humanista Alvar Gómez de Castro, conservadas en el manuscrito 7896 de la BN, sobre cinco conocidas inscripciones. Su interés reside en que constituyen las primeras referencias de cada una de ellas y aportan datos útiles para la historia de las piezas. Pero lo más llamativo es que se presenta una inscripción en S. Román de Hornija con idéntico texto al de la inscripción de S. Juan de Baños. En este trabajo se apuntan algunas hipótesis sobre tan desconcertante y quizás errónea noticia.

  17. Consideraciones sobre la regulación jurídica ambiental de los servicios ecosistémicos en Argentina

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    Clara María Minaverry

    2016-01-01

    Full Text Available El objetivo del presente trabajo es realizar un análisis de los aspectos centrales de la normativa sobre servicios ambientales o ecosistémicos de Argentina, y una breve referencia a la de una selección de países de América Latina (Perú y Costa Rica. Se utilizaron los métodos de observación documental y el de comparación normativa. Uno de los resultados más destacados es que en el último año se han presentado proyectos de leyes ante el Congreso Nacional, cuyos lineamientos generales podrían servir como antecedentes. Sin embargo, en paralelo debería realizarse una evaluación integral respecto a si deben derogarse o modificarse ciertas normas ambientales vigentes, para que no generen nuevas contradicciones. Una de las limitaciones para el caso de Argentina, es que no se dictó una norma específica que regule a los servicios ambientales. Podemos concluir que en la normativa de los otros países de la región que cuentan con leyes sobre pago por servicios ecosistémicos, se pueden encontrar algunos aspectos de relevancia que podrían ser tenidos en cuenta para una futura norma jurídica y/o políticas públicas en Argentina.

  18. El Sistema de Registro de Información sobre Drogas: Un auxiliar diagnóstico en la evaluación de la farmacodependencia

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    JORGE GALVÁN- REYES

    1997-01-01

    Full Text Available El presente trabajo tiene como objetivo dar a conocer la utilidad del Sistema de Registro de Información sobre Drogas como fuente diagnóstica importante para la evaluación del fenómeno de la farmacodependencia en la Ciudad de México. Incluye una breve descripción del soporte metodológico de este sistema, se presentan de manera sintetizada algunos de sus resultados y se discute la importancia de sus datos como material de apoyo para el establecimiento de políticas en materia de investigación y prevención del problema. Se comentan las ventajas de contar con un sistema de este tipo sobre todo porque en nuestro país no existe un sistema de notificación obligatoria de uso de drogas.The aim of this work is to inform of the usefulness of the Drug Information Registry System as an important diagnostic source to evaluate pharmacodependence in Mexico City. A short description of the methodological frame of the system is included, some results are presented synthetically and the importance of these data as supporting material to define research and preventive program policies is discussed. The advantages of these systems are commented, especially in countries, such as Mexico, where notification of drug use is not obligatory.

  19. Puente sobre el Oosterschelde, Holanda

    Directory of Open Access Journals (Sweden)

    Hoving, H. T.

    1967-03-01

    Full Text Available This new bridge, forming part of the Delta project, is made of concrete, and was built by successive overhangs. Precast units were mainly used. The bridge is 5,000 m long, and is 11.85 m wide. The main spans are 95 m in length, except one, which can be opened up to sea traffic, which is 40 m long. The free height of the bridge over the sea is 15 m, which is insufficient for large ships. This required that one of the spans, in deep water, should open up, on hinges, and provide free passage to large ships. The intermediate bridge supports are of special interest. They rest on piles which go down to 38 m. They are hollow, cylindrical, of 4.35 m external diameter, and are driven into the ground in several stages, as they penetrate through the sands at the bottom of the Oosterschelde. To drive them in, an excavating device, inside the pile, was utilised, and also a dredging and suction mechanism, to clean out the bottom, and allow the piles to sink by gravity. But when external friction did not allow this to happen, the piles were weighed down with up to 500 tons of ballast. A series of three of these piles, in a row, makes up each bridge support. A cross member over the pile heads supports, in turn, the bridge deck. The bridge was built in successive sections, overhanging from each support, and then finally connected with a central portion. Use of precast units has been very extensive, so that the bridge can be justiaflably regarded as being prefabricated.Este nuevo puente, que forma parte del Plan de obras denominadas del Delta, es de hormigón y se ha construido por el procedimiento de voladizos sucesivos, utilizando, en su mayor parte, elementos prefabricados. Tiene una longitud total de 5.000 m; una anchura de 11,85 m; tramos principales de 95 m de luz, salvo uno, levadizo, que tiene 40 m. La altura libre sobre las ag^uas del mar es de 15 m, que es insuficiente para las naves de gran tonelaje; esto exigió que uno de sus tramos, en aguas

  20. LEITURA DIGITAL E COMPLEXIFICAÇÃO: REFLEXÕES SOBRE A CONSTITUIÇÃO DE SI

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    Nize Maria Campos Pellanda

    2009-08-01

    Full Text Available Este artigo trata da leitura abordada com ênfase no espaço digital e sustentada nos pressupostos do paradigma da complexidade. O texto inicia com uma caracterização breve da nova cultura digital e suas possibilidades em termos de uma potencialização das condições de construção do sujeito/ construção de subjetividade. A cibercultura torna-se possível a partir de uma abordagem complexa da realidade, ou seja, da consideração de diferentes dimensões dos fenômenos de forma integrada. Nesse sentido, a leitura é considera como uma ação complexa por não separar cognição da constituição de si, no processo de ler. Alguns dados de pesquisa sobre leitura com adolescentes de classes populares são trazidos para ilustrar os elementos teóricos discutidos.

  1. Sobre la formación universitaria del farmacéutico en el Postgrado y Doctorado

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    Mariño EL.

    2012-09-01

    Full Text Available Se pretende con esta publicación clarificar la situación de acceso a los programas de doctorado a los actuales egresados de Farmacia con la nueva titulación de Graduados, dado que, al menos en este tema para ellos, la normativa de hace más de un año es extraordinariamente clara, a diferencia de las opiniones sobre lo que quizás pueda llegar a pasar en otras cuestiones y muy especialmente en lo referente a las conocidas como “competencias”, su certificación ya permite la libre circulación de profesionales en el espacio europeo. Para su mejor comprensión se hace un breve y reciente recorrido de cómo se ha ido modificando, desde no hace mucho tiempo, todo lo relacionado con la realización de la Tesis Doctoral por parte del farmacéutico en general y con el asistencial en particular, partiendo siempre de la premisa de la conveniencia en la facilitación de la consecución de éste que es la mayor de las titulaciones universitarias.

  2. Consideraciones teórico-prácticas Sobre un proyecto de aula: Un ejemplo desde el lenguaje

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    JOSELYN CORREDOR TAPIAS

    2007-01-01

    Full Text Available En Este artículo se presentan, en principio, algunos planteamientos conceptuales sobre el proyecto de aula – enmarcado en la denominada “pedagogía por proyectos. Posteriormente, se proponen “nuevas definiciones” del proyecto de aula, considerándolo como práctica educativa; enseguida, se dan a conocer y se explican en forma precisa y sucinta las funciones y los elementos básicos de este tipo de proyecto pedagógico; aquí, se hace énfasis en la etapa de la elección y delimitación del tema; la formulación problemática, uno de los momentos cumbres del proyecto de aula. Finalmente, estas urdimbres teóricas se materializan en un caso ejemplificador: un proyecto de aula en el marco del léxico infantil, en donde se puede observar como: Proyecto de aula –interés investigativo- ortografía y léxico infantil constituyen un colectivo dinámico, impulsor e inquietante para producir un breve -pero necesario e interesante intento de diccionario infantil de los niños de 5o. Grado.

  3. Somos un envase : Discursos sobre el aborto

    OpenAIRE

    Löcher, Jennifer Irene

    2014-01-01

    No hay persona que no tenga opinión acerca del asunto y, cada vez más, la sociedad argentina debate sobre la pregunta de si se debe legalizar/despenalizar el aborto o no. Nunca antes se habló tanto sobre el tema públicamente: se publican artículos en los diarios, hay manifestaciones a favor y hay manifestaciones en contra de la legalización del aborto. El siguiente trabajo pretende relevar y analizar discursos que circulan socialmente alrededor del debate sobre el aborto. (Párrafo extraíd...

  4. Estudo multicasos sobre atividades inovativas

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    Sonia Regina Hierro Parolin

    2013-09-01

    Full Text Available Em estudos sobre competitividade argumenta-se que as empresas necessitam de estratégias baseadas em inovações e de capacidades internas em constantes e intensas transformações, não somente nos padrões tecnológicos, mas também no encadeamento do processo de gestão das atividades inovativas, como processos dinâmicos, não lineares e diversificados, em função de múltiplos fatores dos ambientes interno e externo. Para contribuir com essas discussões, neste artigo apresenta-se um estudo multicasos em quatro indústrias de médio e grande portes, de segmentos, densidades tecnológicas e históricos com inovações diferentes entre si. Como principais resultados, salientam-se o encadeamento das atividades inovativas como parte de uma estratégia organizacional para obter resultados com inovação e a afluência de todas as pessoas, e não somente as alocadas em pesquisa e desenvolvimento, para o cumprimento dessa estratégia.

  5. História e memória da Revolução Farroupilha: breve genealogia do mito

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    Jocelito Zalla

    2011-12-01

    Full Text Available O objetivo deste texto é apresentar um panorama das manifestações em torno da Revolução Farroupilha, desde o próprio episódio até sua consolidação como mito fundador da identidade regional no Rio Grande do Sul. Debatendo momentos importantes desse processo, como o da refração à memória sobre o evento, o de sua apropriação política pelo projeto republicano, sua reabilitação pela memória histórica e sua ritualização pelo tradicionalismo gaúcho, propõe-se reconstituir analiticamente a fixação do fato como patrimônio simbólico coeso e múltiplo, suficientemente elástico para pautar conflitos internos e tensões entre o estado e o centro do Brasil.

  6. Ampliação da técnica violonística de mão esquerda: um estudo sobre a pestana Extended left-hand guitar technique: a study on the barré

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    Bruno Madeira

    2013-06-01

    Full Text Available A técnica instrumental é um elemento da performance musical em constantes reformulações. Com o objetivo principal de estender a técnica violonística da pestana, o presente artigo foi dividido em três partes: (1 conceituações gerais sobre técnica instrumental; (2 a partir de métodos instrumentais dos últimos quatrocentos anos, um breve histórico da pestana foi delineado com o objetivo de recolher informações técnicas sobre sua realização, (3 sugestões e exemplificações de procedimentos não usuais de pestana (com dedos diferentes do indicador, de falange, bisagra e cruzada, encontrados de forma muito resumida na escassa bibliografia sobre o assunto e cuja aplicabilidade é verificada no repertório.Instrumental technique is an element of musical performance in constant reformulation. With the main objective of expanding the guitar technique of barring, this study was divided into three parts: (1 general concepts about instrumental technique; (2 from instrumental methods of the past four hundred years, a brief history of the barré was outlined in order to collect technical information about its realization, (3 suggestions and examples of unusual barring procedures (with different fingers, phalanx barré, hinge barré, crossed barré, found in the scarce bibliography and which applicability is verified in the repertoire.

  7. A nova edição de Why Suyá Sing, de Anthony Seeger, e alguns estudos recentes sobre música indígena nas terras baixas da América do Sul

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    Luís Fernando Hering Coelho

    2007-04-01

    Full Text Available Lançado originalmente em 1987, Why Suya Sing? ganhou uma nova edição norte-americana em 2004. Apesar de constituir um marco teórico e etnográfico importante nos campos da etnologia indígena e da antropologia da música, o livro ainda não conta com uma tradução para o português. Neste ensaio, oferece-se uma leitura da obra, buscando resumir alguns de seus pontos principais, junto a uma contextualização geral sumária dos estudos sobre povos Gê, e uma nota breve sobre alguns desenvolvimentos recentes nas pesquisas sobre música indígena nas terras baixas da América do Sul.Originally published in 1987, Why Suyá Sing? acquired a new US edition in 2004. Despite constituting a theoretical and ethnographic landmark in the fields of indigenous ethnology and anthropology of music, Seeger’s book has yet to be translated into Portuguese. This essay offers a reading of the work, looking to resume some of its key points, along with a brief overall contextualization of the studies on Gê peoples, and a short note on some of the recent developments in the research on indigenous music in the South American lowlands.

  8. Los derechos de autor sobre obtenciones vegetales

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    Rosario Silva Gilli

    2015-01-01

    Full Text Available Contenido: Las creaciones en la agricultura. Los derechos de autor sobre obtenciones vegetales. El Convenio Internacional para la protección de las obtenciones vegetales (París 1961 y sus revisiones de Ginebra 1972, 1978,1991 Objeto de la protección legal. Condiciones del cultivar. Alcance de la protección. La protección de obtenciones vegetales en el Mercosur. Los Derechos de autor en el Mercosur. El Protocolo de Armonización de normas sobre Propiedad Intelectual en el Mercosur. El Acuerdo de Cooperación y facilitación sobre la protección de las obtenciones vegetales en los Estados Partes del Mercosur.  Alcance de la normativa Mercosur. Los Derechos sobre cultivares en Uruguay. Los Derechos de autor como derechos humanos. ¿Derechos de autor vs. derecho de propiedad intelectual? Reflexión final

  9. Conocimientos sobre incontinencia urinaria en pacientes hospitalizados

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    Otto Guillen Lopez

    2003-10-01

    Full Text Available Objetivo: Determinar el nivel de conocimientos sobre incontinencia urinaria (IU en pacientes hospitalizados. Material y métodos: Estudio transversal, descriptivo; se entrevistó una muestra por saturación de 325 pacientes hospitalizados de 30 años a más del Hospital Nacional Cayetano Heredia (HNCH, Lima - Perú, utilizando un cuestionario de conocimientos sobre incontinencia, considerándose como adecuado conocimiento cuando había más de 50% de aciertos en él. Resultados: Solamente 35.7% tuvieron conocimiento adecuado sobre IU. Hasta 90% conocía acerca del éxito del tratamiento y posibilidad de curación de los pacientes incontinentes. Sin embargo, 75% consideraron erróneamente que la IU era consecuencia inevitable del envejecimiento, mientras menos de 50% de pacientes conocía el papel de ciertos medicamentos y ejercicios en la IU. Conclusiones: Se demostró un pobre conocimiento general sobre la IU, siendo necesaria una intervención de los profesionales de la salud en la educación de la población, sobre todo hospitalizada y con factores predisponentes para desarrollar IU, para así poder modificar las ideas erróneas que existen sobre el tema. (Rev Med Hered 2003; 14: 186-194.

  10. Considerações sobre o narcotráfico no Marajó: reflexões sobre o desemprego e a ilegalidade (Considerations on the drug trafficking in Marajó: reflections on unemployment and illegality

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    Christiane Pimentel Silva

    2011-01-01

    Full Text Available Resumo: O mercado de trabalho contemporâneo mostra crescente incapacidadede absorver força de trabalho, mesmo a qualificada, em razão da reestruturação produtiva, que amplia em proporção geométrica as formas de trabalho precárias e os níveis de desemprego. O resultado dessas alterações na vida social foi o aprofundamento na concentração de renda e redimensionamento das condições de reprodução da população e expansão sem precedentes da pobreza no Brasil e no mundo. A pesquisa realizada no município de Breves, no arquipélago do Marajó, parte da avaliação do desenvolvimento social do município, analisando os indicadores oficiais sobre renda, índice de pobreza, emprego, informalidade, IDH, esgotamento sanitário, etc. Também foram realizadas entrevistas com 11 pessoas envolvidas no mercado ilícito. O resultado permite compreender que, sob contexto de vulnerabilidade social, as redes do narcotráfi co conseguem se desenvolver, utilizando-se da mão de obra expulsa do mercado formal, que possui pouca possibilidade de retorno, por sua baixa qualifi cação e escolaridade.Abstract: Contemporary labor market reveals an increasing inability to absorb even qualified workforce due to the restructuring of production methods which expands in great proportions the precarious forms of employment and unemployment levels. The results of these changes in social life are: income concentration, changes in the reproduction conditions of the population; and an unprecedented expansion of poverty in Brazil and worldwide. The research conducted in the municipality of Breves, located in the archipelago of Marajó, started from the evaluation of social development of the city, examining the official income indicators, poverty rate, employment, informal market, HDI and basic sanitation. The research also included interviews with eleven people involved in the illegal market. The result reveals that a context of social vulnerability enables the

  11. PERSPECTIVAS INFANTILES SOBRE LA TERRITORIALIDAD

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    NOELIA ENRIZ

    2009-12-01

    Full Text Available In this paper we propose a different approach to children's ideas about the geographical area in the particular context of the Mbyá Guarani population, from Misiones (Argentine. The territorial fragility, that is exposed to the indigenous people in Argentina aremanifested daily in several areas. Mbyá population, has been crossed by the boundaries of national states of Paraguay, Brazil and Argentina. The sedentary and enclosures land processes in different states, have prompted changes in the logic of subsistence, housing and community organization. In Misiones province case, bureaucratic accreditation of land titles is postponed, the state is not committed to the realization of this right.RESUMEN: En este trabajo nos proponemos un acercamiento a las diversas ideas infantiles sobre el territorio habitado, en el contexto particular de la población mbyá guaraní de Misiones. La fragilidad territorialidad a la que es expuesta la población indígena en Argentina se manifiestan cotidianamente en diversas áreas. La población mbyá, ha sido surcada por los límites de los estados nacionales de Paraguay, Brasil y Argentina. La sedentarización y los cercamientos de tierras en los diferentes estados, han impulsado transformaciones de las lógicas de subsistencia, de habitación y organización comunitaria. En el caso de la provincia de Misiones la acreditación burocrática de los títulos de tierras se encuentra aplazada, el estado no se compromete con la concreción de este derecho.

  12. Consideraciones preliminares sobre la credibilidad

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    Fernando Verdugo

    2004-01-01

    Full Text Available A modo de introducción al Seminario Interno de Profesores de la Facultad de Teología de la PUC, el autor recuerda, en primer lugar, lo que suele entenderse por "credibilidad" y, también, algunos de los criterios generales por los cuales algo o alguien adquiere la calidad de creíble (el que sea razonable, relevante o significativo, capaz de producir gozo estético, etc.. Luego, en la parte más extensa, el autor vuelve a llamar la atención sobre la necesidad de atender a los condicionamientos culturales de la fe cristiana. La fe, al igual que toda experiencia humana, está culturalmente mediada. Tomar conciencia de ese hecho y asumir las consecuencias metodológicas es indispensable para una teología que quiere poner atención a los desafíos actuales y contribuir así a la credibilidad del cristianismoThis article reprints the introductory session to the internal seminar of lecturers from the Faculty of Theology of the UC. In the first place, the author sums up what is usually understood by `credibility' and the general criteria through which something or someone gains credibility (being reasonable, relevant or significant, capable of producing aesthetic pleasure. Then the author deals at length with the need to take notice of the cultural conditionings of the Christian faith. The faith, the same as any other human experience, is culturally mediated. It is crucial for theology to become aware of this fact and come to terms with its methodological consequences, if it wants to face current challenges and so contribute to the credibility of Christianity

  13. Potencial Expressivo nos Jogos Indie e Gamearte: Breve análise dos jogos Flower e Journey

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    Bruno Henrique De Paula

    2013-09-01

    Full Text Available Os jogos digitais tem se apresentado como um meio com grande potencial expressivo e comunicacional, contrariando a visão tradicional que considera os games como uma mídia que deve ser usada apenas para o entretenimento. Nos jogos independentes – indies – é possível encontrar maiores possibilidades de experimentação, o que favorece uma mudança no paradigma tradicional. Dentro deste universo indie estão inseridos os jogos Flower e Journey que introduziram interessantes inovações, um padrão estético diferencial e inovador e, também, atingiram um grande sucesso comercial e de crítica, abrindo novas referências de como se encarar os jogos digitais. O objetivo deste trabalho é refletir sobre as experiências proporcionadas por estes jogos, evidenciando como essas criações e inovações foram possíveis e como estes games se destacaram de outras produções da área. The Expressive Potential of Indie Games and Game Art: Brief analysis of Flower and Journey videogames  Abstract Digital games have been shown to be a powerful expressive and communicative medium, struggling against the traditional view of games as shallow media. It’s especially at indie games space that there has been a greater experimentation, shifting this paradigm. Flower and Journey are in this indie universe, and these games have reached a great success in many spheres, helping this paradigm shift and favoring a new way to face digital games. The objective of this work is to reflect about the gameplay experience of both games, showing how these games innovated and helping to understand why these games have reached an important position on last years. Keywords: Digital games; indie games; expression in games; aesthetics of games; digital interfaces.

  14. Tendência Pedagógica Progressista Libertária – Uma breve apresentação

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    Kathlen Luana de Oliveira

    2011-10-01

    Full Text Available O presente texto versa sobre a tendência pedagógica progressista libertária. Tem como objetivo apresentar o contexto no qual surgiram os princípios libertários para, então, abordar as críticas e a proposta educacional dessa tendência. Brevemente, será exposta a Pedagogia Libertária norteada pela anarquia, pela crítica institucional, porém com propostas de práticas educativas construídas a partir da autogestão e da solidariedade. Nesse sentido, as contribuições de Lobrot, Tragtenberg serão delineadas frente aos desafios concretos e possíveis da proposta libertária.

  15. Avaliação da influência da temperatura sobre o desenvolvimento de Aedes albopictus

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    Daniéla Cristina Calado

    2002-04-01

    Full Text Available OBJETIVO: Verificar a influência da temperatura sobre os estágios de desenvolvimento de Aedes albopictus sob condições de laboratório. MÉTODOS: Foi avaliado o período de desenvolvimento dos estágios de ovo, larva e pupa em quatro temperaturas constantes de 15ºC, 20ºC, 25ºC e 30ºC em câmaras climatizadas com umidade relativa do ar oscilando entre 70% e 85% e fotoperíodo de CE 12:12 horas. Pela análise de variância, foram testados os efeitos das quatro temperaturas constantes. RESULTADOS: O período de incubação dos ovos compreendeu 38,38; 19,09; 13,10; e 10,44 dias; o período larval dos indivíduos machos atingiu 30,13; 13,83; 7,36; e 5,57 dias; o período larval para fêmeas apresentou duração de 33,22; 15,00; 8,06; e 6,16 dias; o período pupal em machos compreendeu 8,01; 4,92; 2,40; e 1,76 dias, enquanto nas fêmeas foi de 8,15; 5,11; 2,60; e 1,88 dias sob as temperaturas de 15ºC, 20ºC, 25ºC e 30ºC, respectivamente. Em todos os estágios de desenvolvimento, foram encontradas diferenças significativas, entre as temperaturas avaliadas, em torno de 5% no tempo de desenvolvimento. O período de desenvolvimento foi inversamente relacionado à temperatura, com o período larval mais breve em machos que em fêmeas. Nas temperaturas de 20ºC a 30ºC, o desenvolvimento foi mais breve e com maior viabilidade que a 15ºC. CONCLUSÕES: Nas condições em que foram realizados os experimentos, a temperatura afetou de forma significativa e inversamente proporcional os estágios imaturos de Aedes albopictus. Esses resultados mostram que os estágios imaturos são mais suscetíveis durante os períodos de baixas temperaturas devido ao maior tempo necessário para completar o desenvolvimento, informação que pode ser utilizada em estratégias de controle.

  16. Isolation, identification and characterisation of three novel probiotic strains (Lactobacillus paracasei CNCM I-4034, Bifidobacterium breve CNCM I-4035 and Lactobacillus rhamnosus CNCM I-4036) from the faeces of exclusively breast-fed infants.

    Science.gov (United States)

    Muñoz-Quezada, Sergio; Chenoll, Empar; Vieites, José María; Genovés, Salvador; Maldonado, José; Bermúdez-Brito, Miriam; Gomez-Llorente, Carolina; Matencio, Esther; Bernal, María José; Romero, Fernando; Suárez, Antonio; Ramón, Daniel; Gil, Angel

    2013-01-01

    The aim of the present study was to isolate, identify and characterise novel strains of lactic acid bacteria and bifidobacteria with probiotic properties from the faeces of exclusively breast-fed infants. Of the 4680 isolated colonies, 758 exhibited resistance to low pH and tolerance to high concentrations of bile salts; of these, only forty-two exhibited a strong ability to adhere to enterocytes in vitro. The identities of the isolates were confirmed by 16S ribosomal RNA (rRNA) sequencing, which permitted the grouping of the forty-two bacteria into three different strains that showed more than 99 % sequence identity with Lactobacillus paracasei, Lactobacillus rhamnosus and Bifidobacterium breve, respectively. The strain identification was confirmed by sequencing the 16S-23S rRNA intergenic spacer regions. Strains were assayed for enzymatic activity and carbohydrate utilisation, and they were deposited in the Collection Nationale de Cultures de Microorganismes (CNCM) of the Institute Pasteur and named L. paracasei CNCM I-4034, B. breve CNCM I-4035 and L. rhamnosus CNCM I-4036. The strains were susceptible to antibiotics and did not produce undesirable metabolites, and their safety was assessed by acute ingestion in immunocompetent and immunosuppressed BALB/c mouse models. The three novel strains inhibited in vitro the meningitis aetiological agent Listeria monocytogenes and human rotavirus infections. B. breve CNCM I-4035 led to a higher IgA concentration in faeces and plasma of mice. Overall, these results suggest that L. paracasei CNCM I-4034, B. breve CNCM I-4035 and L. rhamnosus CNCM I-4036 should be considered as probiotic strains, and their human health benefits should be further evaluated.

  17. Breves anotações sobre a carnavalização e alguns de seus disfarces em dois filmes latinoamericanos: Como água para chocolate e Carlota Joaquina

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    Eduardo Peñuela Cañizal

    2005-12-01

    Full Text Available The author of this article sets out to examine, as an inventive process, some characteristic ways of camavalisation that have take shape in two Latin American films. He explores also the desire that involves the constmctions of images and visual narrative.

  18. LAS LIBERTADES DE EXPRESIÓN Y DE REUNIÓN EN LA CONSTITUCIÓN ESPAÑOLA: BREVE APUNTE SOBRE LOS “ESCRACHES” COMO PUNTO DE CONFLUENCIA ENTRE AMBAS LIBERTADES

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    Mercè Barceló i Serramalera

    2013-12-01

    Full Text Available El derecho de reunión es una manifestación colectiva de la libertad de expresión y, por tanto, garantía de una opinión pública libre; y garantía, a su vez, del principio democrático participativo, como la libertad de expresión. La conexión simbiótica entre ambos derechos fundamentales es el objeto principal de estas páginas que, tras el examen individual de cada derecho, buscarán los puntos de convergencia entre ambos; en concreto, a través de los llamados “escraches”.  El “escrache”, como se verá, no es más que una modalidad de ejercicio del derecho de reunión a través del que se potencia la libertad de expresión. Pero, el hecho de haber sido designado con ese nombre, especialmente por los medios de comunicación, ha propiciado que se olvide, a menudo, su naturaleza de derecho fundamental y se le identifique con una conducta molesta y perturbadora capaz de vulnerar derechos fundamentales de los afectados. En este trabajo, sin embargo, se atiende a la naturaleza jurídica de los llamados “escarches” y se formulan criterios de resolución de conflictos entre ese y otros derecho en caso de colisión. Y se propone, así, que el “escrache”, como las libertades de expresión y el mismo derecho de reunión, posee un valor preponderante frente a los derechos del ámbito privado. De modo que sólo a través de una adecuada ponderación que parta de este presupuesto y utilice criterios interpretativos derivados de la singular faceta objetiva del “escrache” (garantía de la opinión pública libre y del principio democrático participativo puede limitarse constitucionalmente esa forma de ejercicio del derecho fundamental de reunión. Palavras clave: Libertad de expresión. Derecho de reunión. “Escrache”. Derecho fundamental.

  19. Breve Reseña sobre el Libro “Tema de Ética Médica” de que es Autor el Académico Fernando Sánchez Torres

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    Mario Camacho Pinto

    1995-08-01

    Full Text Available

    La presentación de este edificante libro en reciente sesión especial de la Academia Nacional de Medicina constituyó toda una inolvidable reunión pues su autor hizo gala de un estilo fluido y diserto, fruto de un dominio del Tema que iluminó con filminas demostrativas de una evidencia objetiva y laboriosa que alcanza holgadamente el grado de excelencia. Porque progresivamente nos fue interiorizando en el contenido de todos y cada uno de los XX capítulos de que consta su obra, tratados en forma actualizada, coherente y práctica. Posteriormente con la lectura de su texto complementé la información que me he permitido hacer para los lectores de MEDICINA, en forma sucinta, un resumen acerca de lo que allí se enseña.

    Su enfoque es universal respaldado por numerosas citas bibliográficas tomadas de la literatura profana y médica mundialmente reconocidas y traídas al texto en forma oportuna y contributoria.

    Capítulos I y 11. Ética general, ética

    Médica y Bioética

    Escudriñando estos dos Capítulos nos encontramos con una afortunada descripción evolutiva de los principios y valores morales desde los griegos con su criterio hipocrático tradicional, impregnado de Metafísica, a más de naturalismo, que con la progresiva evolución conceptual a través de los siglos, de orden científico, social e individual ha venido colocando la Ética Médica en una nueva era de Derechos y Deberes para los médicos, pacientes, sociedad, Estado, situación activada últimamente por los progresos científicos y tecnológicos en las diversas áreas de la Biología, tanto experimental como de investigación, Biología Molecular, Biomedicina, Biotecnología, Bioingeniería, Ecología que llevaron al sensacional descubrimiento de la BIOÉTICA en 1971 por el norteamericano POTTER, preocupado por cómo actuar ante la Académico Mario Camacho Pinto creciente brecha abierta entre las prospeccioes experimentales de los Biólogos y los inmanentes valores éticos que garantizan la vida.

    Capítulos III y IV Cómo debiera ser el médico y ética y formación médica

    Ambos constituyen una síntesis de sendas conferencias del Autor en que proclama la excelencia de la Medicina que eleva a la categoría de Misión cuyo cometido sólo puede ser llevado a cabo por un profesional culturalmente educado y científicamente preparado para tal fin . Serán estos dos capítulos de sumo interés para educadores interesados en dar adecuada formación y oportuna orientación profesional a sus alumnos.

    Capítulos V y VI. La ética en la formación del personal de salud y la ética médica en la humanización de salud

    Son dos capítulos definitivamente indispensables e imprescindibles de conocer en Odontología, Enfermería, Psicología, Terapias, Rehabilitación y Nutrición. Su lectura debiera ser obligatoria para el infundir moral, vale decir ética en estos profesionales...

  20. Actor-Network Theory as I image it. Brief essay on traffic lights La ANT tal como yo la imagino. Breve ensayo sobre el cosmos semafórico

    Directory of Open Access Journals (Sweden)

    Carlos Enrique Silva Rios

    2011-03-01

    Full Text Available Three years ago I came to Barcelona with a firm intention that I had to change after about two months from my arrival. I wanted to study people crossing the street in this city and then compare their actions with those of the people in my hometown -  Caracas. Since carrying out a comparison between two cities was time consuming, I decided to do my research in Barcelona only. Later on, for studying people crossing the street, I decided to consider traffic lights as a starting point. Then I undertook the task of preparing a field diary based on some of the notions of Actor-Network Theory as Bruno Latour puts them. I did not use his approach as a systematic set of concepts, but as a device for imagination, i.e., as a number of ways that promote and facilitate the re-creation of worldly events. In this essay, I briefly present a part of my research experience, and I focus on the following idea: we can compose a common world, a cosmos, from a seemingly discrete entity, in my case, a traffic light. Hace tres años llegué a Barcelona con una intención firme que pasados unos dos meses tuve que echar por tierra. Quería estudiar a la gente cruzando la calle en esta ciudad y luego comparar sus actos con los de la gente de mi ciudad de origen, Caracas. Mis evaluadores, apenas se enteraron de mi propósito, me desaconsejaron, ya que comparar requería de un tiempo que no tenía. Debía dedicarme sólo a una de las ciudades. Escogí Barcelona. Más tarde, en una segunda consulta, volvieron a desaconsejarme: en lugar de estudiar a la gente que cruza la calle, me recomendaban que tomara como punto de partida el semáforo; específicamente, el cambio de luz y si era posible la luz misma. Esta especie de atomización del interés, en lugar de amilanarme, redefinió y acicateó mi entusiasmo cogitativo, y con ese impulso emprendí la tarea de elaborar un diario de campo. Como no se podía hacer eso de una manera ingenua, tomé la decisión de asumir una mirada y luego una escritura sesgada por algunas de las nociones de la Teoría del Actor-Red tal como las plantea Bruno Latour. Su manera de ver el mundo, más que recibirla como un conjunto sistemático de conceptos, la he incorporado muy lentamente como un dispositivo de imaginación, es decir, como una serie de formas que promueven y facilitan la re-creación de los acontecimientos mundanos. En este ensayo hablo brevemente de parte de esa experiencia investigativa, centrándome en una idea que para mí no deja de ser fascinante: es posible componer un mundo común, un cosmos, partiendo de una entidad aparentemente discreta; en mi caso, el semáforo.

  1. ENTRE A FICÇÃO E A OBJETIVIDADE breve estudo sobre a crônica no jornalismo brasileiro durante a Copa do Mundo de Futebol de 2002

    Directory of Open Access Journals (Sweden)

    José Carlos Marques

    2012-08-01

    Full Text Available A Copa do Mundo de Futebol de 2002, disputada no Japão e naCoreia do Sul, representava um desafio inédito para a coberturados jornais brasileiros: oferecer um conteúdo atraente para oleitor diante dos prazos de edição e publicação, os quaisantecediam em poucos momentos os horários das partidas dotorneio. Uma das estratégias para combater a efemeridade dotempo da notícia foi a de recorrer à colaboração de escritores ecronistas. Convocados para comentar o evento, essesprofissionais distanciaram-se da mera referência jornalística;assim, fatos e notícias reconstruídos passaram a adquirircontornos carregados de subjetividade e criação literária.

  2. Matrimonio, sexualidad y bioética en el magisterio pontificio: de Pío XI a Francisco. Breves consideraciones históricas sobre una enseñanza insistente y prolífica

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    Rodolfo R. de Roux

    Full Text Available Resumen Este artículo explica el porqué del interés creciente de los papas, a partir de 1930, en temas relativos al matrimonio, a la sexualidad y a la bioética. Se ponen de relieve algunos aspectos importantes de los principales documentos del magisterio pontificio en la materia, se los contextúa históricamente y se señalan las persistencias y también las evoluciones en el discurso papal.

  3. Breves reflexões sobre a transposição das directivas de contratação pública de 2014: precisaremos de um novo Código dos Contratos Públicos?

    OpenAIRE

    Caldeira, Marco

    2015-01-01

    As novas Directivas comunitárias em matéria de contratação pública obrigarão o legislador Português a proceder à revisão do Código dos Contratos Públicos. No texto apela-se a que essa transposição, que traduz uma decisão política, não seja feita de forma acrítica mas sim de forma participada e ponderada, aproveitando-se para alterar os aspectos do Código que careçam de aperfeiçoamento mas sem cair na tentação (e no erro) de proceder à elaboração de um novo Código. The Portuguese Government...

  4. Brief sketch on: The evaluation, modifications, effects, attenuations and monitored of the environmental impact produced by the mining; Breve esbozo sobre: La evaluacion, modificaciones, efectos, atenuaciones y monitoreo del impacto ambiental producido por la mineria

    Energy Technology Data Exchange (ETDEWEB)

    Antonio, Gaviria Rivera

    1992-07-01

    Any type of mining methodology is unavoidable and in the majority of the cases irrevocable due to the great necessity of row materials demanded by the consumption increasing and progress. for these reasons this activity is constantly causing damages against either t the community t or the nature it self or both. The only feasible solution to diminish this damage is to take the proper precautions to decrease the resultant degradation and impact. If it is possible to set them into tolerable levels, which must be very close to the existing environmental norms.

  5. Breve estudio sobre la influencia de la History of Spanish Literature de George Ticknor en manuales posteriores: aparición y desaparición de las mujeres en el canon literario.

    Directory of Open Access Journals (Sweden)

    Gemma Delicado Puerto.

    2008-09-01

    Full Text Available Resumen: George Ticknor, autor de una Historia de la literatura española (1849, que comprende desde el s. XIII hasta principios del s. XIX, ha influido sobremanera en manuales de literatura posteriores y, por ende, en el canon literario oficial. Ticknor y más tarde el erudito británico James Fitzmaurice-Kelly borran de la historia de la literatura española a numerosas escritoras por dos motivos: al no incluir sus nombres en la obra y de los incluidos hacer referencias negativas en la mayoría de los casos. En este artículo se analizan estas ausencias, las referencias negativas, los posibles motivos que parecen haber llevado al autor a hacerlas y las consecuencias de todo ello en manuales posteriores.Summary: George Ticknor, author of a History of Literature that covers the period from early 18th century to the XIX, has influenced in later literature manuals, and therefore in the official literary canon. Ticknor and later on the British scholar James Fitzmaurice-Kelly contributed to erase the names of a great number of women writers from the history of Spanish literature for two main reasons: by no including their names in the manual and comment negatively most of those included. Absences, negative comments, and the possible reasons that lead the authors to do so, together with the consequences of these facts are analyzed in this article.

  6. Breve relación histórica sobre la formación y el desarrollo de la noción de derecho definido como facultad o potestad («derecho subjetivo»

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    Alejandro GUZMÁN-BRITO

    2013-07-01

    Full Text Available The paper describes the key milestones in the history of the notion of legal right, modernly called «subjective». From an argument regarding the absence of that notion in the science of the Roman jurists and their likely origin at the Middle Ages, we examine the contributions of Marsilio de Padua, Guillermo de Ockham and Juan Gerson, on one hand, and Francisco de Vitoria and the sixteen century’s NeoScholastic one the other, in which Hugo Grotius founded to introduce the concept in the rationalist natural law of the seventeenth and eighteenth centuries, than collected from the German Pandectism of the next century, expanding it universally. By this study it is showed that the idea of subjective right lies essentially in the right or faculty, an attribute belonging to the category of quality, which explains that in the past it has been attributed to animals and inanimate, which also have facultative attributes, and that today rights are being claimed under the view of having right to what is claimed.

  7. Entre a recriação e a renúncia, o ato de deixar falar: breve abordagem sobre produtividade poética brasileira e polifonia a partir de Chico Alvim e Carlos Nejar

    Directory of Open Access Journals (Sweden)

    Cinthia Maritz dos Santos Ferraz Machado

    2016-08-01

    Full Text Available Este artigo propõe uma abordagem acerca da produtividade literária e da polifonia na poética brasileira contemporânea a partir de autores como Chico Alvim e Carlos Nejar. Pretendemos demonstrar que processos de criação configuram as obras destes poetas que têm em comum a característica do ato de deixar falar: de conferir ou imprimir vozes às suas criações num processo de ânima. Chico Alvim e Carlos Nejar manejam o discurso fora da ordenação ou da hegemonia. Inscrevem-no na expressão da pluralidade por meio de um movimento ou jogo dinâmico; do processo contínuo e não de um sistema operante e determinante. Em seus poemas estão inscritas a dramatis personae, responsável pelo trânsito entre o olhar e o deixar falar, em direção ao leitor.

  8. “De límites y potencias”. Breve ensayo sobre la relación entre ley y soberanía popular en la teoría liberal y republicana

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    Ignacio Luis Moretti

    2015-09-01

    Full Text Available ¿Puede efectivamente hablarse de soberanía del pueblo, si la misma debe someterse a los dictados de un procedimiento de orden superior (la constitución o el imperio de la ley? Esta es la pregunta central que pretende exponer la inherente tirantez constituyente de la soberanía popular y su relación con la ley. Lejos de constituirse en una relación simple o lineal para el pensamiento político, la misma plantea diversas problemáticas que deben ser explicitadas y analizadas. Este pequeño ensayo pretende esbozar un análisis crítico de esta relación, tomando para ello a los principales exponentes de la teoría política liberal y republicana. Relación, por último, no menor no sólo para el propio pensamiento político, sino para un contexto latinoamericano donde los procesos democráticos ponen continuamente en tensión el espacio de la institucionalidad y su relación con el poder popular.

  9. Consenso mexicano sobre la dispepsia

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    R. Carmona-Sánchez

    2017-10-01

    Full Text Available Desde la publicación de las guías de dispepsia 2007 de la Asociación Mexicana de Gastroenterología ha habido avances significativos en el conocimiento de esta enfermedad. Se realizó una revisión sistemática de la literatura en PubMed (01/2007 a 06/2016 con el fin de revisar y actualizar las guías 2007 y proporcionar nuevas recomendaciones basadas en evidencia. Se incluyeron todas las publicaciones en español e inglés, de alta calidad. Se redactaron enunciados que fueron votados utilizando el método Delphi. Se estableció la calidad de la evidencia y la fuerza de las recomendaciones según el sistema GRADE para cada enunciado. Treinta y un enunciados fueron redactados, votados y calificados. Se informan nuevos datos sobre definición, clasificación, epidemiología y fisiopatología. La endoscopia debe realizarse en dispepsia no investigada cuando hay datos de alarma o falla al tratamiento. Las biopsias gástricas y duodenales permiten confirmar infección por Helicobacter pylori y excluir enfermedad celiaca, respectivamente. Establecer una fuerte relación médico-paciente, cambios en la dieta y en el estilo de vida son útiles como medidas iniciales. Los bloqueadores H2, inhibidores de la bomba de protones, procinéticos y fármacos antidepresivos son efectivos. La erradicación de H. pylori puede ser eficaz en algunos pacientes. Con excepción de Iberogast y rikkunshito, las terapias complementarias y alternativas carecen de beneficio. No existe evidencia con respecto a la utilidad de prebióticos, probióticos o terapias psicológicas. Los nuevos enunciados proporcionan directrices basadas en la evidencia actualizada. Se presenta la discusión, el grado y la fuerza de la recomendación de cada uno de ellos.

  10. Desarrollo de un Cuestionario Breve para la Medición del Comportamiento Arriesgado en Peatones Adolescentes/ Development of a Brief Questionnaire to Measure Risky Behavior in Adolescent Pedestrians/ Desenvolvimento de um Questionário Breve para a Medição do Comportamento Arriscado em Pedestres Adolescentes

    Directory of Open Access Journals (Sweden)

    DAVID HERRERO-FERNÁNDEZ

    2015-10-01

    Full Text Available El objetivo del presente estudio fue la creación de un cuestionario autoinformado breve para valorar los comportamientos arriesgados en peatones. Para ello se contó con una muestra de 351 personas que completaron los 13 ítems iniciales del instrumento y que llevaron a cabo una tarea de percepción de riesgo a modo de medida de validez. El análisis factorial confirmatorio verificó el ajuste satisfactorio del modelo final de 8 ítems en un único factor, que alcanzó una consistencia interna satisfactoria (α=.75. Posteriormente los análisis evidenciaron la validez convergente y discriminante del cuestionario, con base en las correlaciones obtenidas con las medidas de percepción de riesgo administradas.

  11. Escribir los caminos. La construcción del espacio en la Descripción breve... de Reginaldo de Lizárraga

    Directory of Open Access Journals (Sweden)

    Silvia Tieffemberg

    2014-06-01

    Full Text Available El trabajo propone el análisis de la construcción del espacio en la Descripción breve de toda la tierra del Perú, Tucumán, Río de la Plata y Chile, escrita por Reginaldo de Lizárraga probablemente entre 1591 y 1603. El concepto de espacio geográfico se define a partir de la articulación entre la naturaleza y el elemento humano, tanto aborigen como producto de la inmigración voluntaria o forzada. Se distingue, además, un espacio no geográfico, coexistente e interactuante con el anterior, el cual –denominado espacio trascendente– se define como la articulación entre el elemento humano y el supratopos. En síntesis, el trabajo propone un análisis de la obra en la perspectiva de un desplazamiento que va de lo descriptivo a lo prescriptivo, y permite una lectura política de los espacios americanos.

  12. BREVE VISIÓN DE LOS PRINCIPALES PROTAGONISTAS DE LA ESCUELA INGLESA DE ATENCIÓN PRIMARIA DURANTE EL ÚLTIMO MEDIO SIGLO

    Directory of Open Access Journals (Sweden)

    Carlota Blanco Rubio

    2016-01-01

    Full Text Available El objetivo es hacer un breve repaso de las influencias, el núcleo duro de perfil clínico, la enfermería científica y el presente de la Escuela Inglesa de Atención Primaria durante los últimos sesenta años. Entendemos que la influencia intelectual más profunda fue la de Archie Cochrane. Entre los médicos de familia componentes del núcleo duro destacamos a John Fry (1922- 1994, David Metcalfe y Julian Tudor Hart. Por otra parte, Lisbeth Hockey (1918-2004, doctora en enfermería, fue la responsable durante varios años de la Unidad de Investigación en Cuidados de la Universidad de Edimburgo. Las fuentes de investigación historiográfica de la enfermería inglesa en internet son notables. Finalmente, Trisha Greenhalgh, médica de familia, investigadora y docente en la Universidad de Oxford, está lanzando a la vieja Escuela Inglesa de Atención Primaria hacia la mediana del siglo XXI.

  13. Enhancement of ginsenoside Rg(1) in Panax ginseng hairy root by overexpressing the α-L-rhamnosidase gene from Bifidobacterium breve.

    Science.gov (United States)

    Zhang, Ru; Zhang, Bian-Ling; Li, Gu-Cai; Xie, Tao; Hu, Teng; Luo, Zhi-Yong

    2015-10-01

    To improve the production of ginsenoside Rg1 in Panax ginseng. The α-L-rhamnosidase gene from Bifidobacterium breve (BbRha) was overexpressed into hairy root culture system using Agrobacterium rhizogenes A4. Ginsenoside Rg1 in hairy roots was obtained following transformation via overexpressed gene representing 2.2-fold higher than those of control lines. Several overexpression transgenic hairy root lines were obtained exhibiting markedly increased levels of the corresponding α-L-rhamnosidase enzymatic activity relative to control. Ginsenoside Rg1 levels in the transgenic lines were higher (2.2-fold) than those of control after following 30 days culturing, while ginsenoside Re contents in tested transgenic lines were found to be lower. The transgenic hairy roots harboring α-L-rhamnosidase gene improved the accumulation of ginsenoside Rg1 up to 3.6 mg g(-1) dry weight. BbRha gene selectively enhances the production of ginsenoside Rg1 in P. ginseng hairy roots.

  14. Bifidobacterium breve IPLA20005 affects in vitro the expression of hly and luxS genes, related to the virulence of Listeria monocytogenes Lm23.

    Science.gov (United States)

    Rios-Covian, David; Nogacka, Alicja; Salazar, Nuria; Hernández-Barranco, A M; Cuesta, Isabel; Gueimonde, Miguel; de Los Reyes Gavilán, Clara G

    2018-03-01

    Mechanistic features that characterize the interaction and inhibition of the food-borne pathogen Listeria monocytogenes by members of the genus Bifidobacterium still remain unclear. In the present work, we tried to shed light on the influence that co-cultivation of L. monocytogenes with Bifidobacterium breve may exert on both microorganisms and on virulence of the pathogen. Production of acetate and lactate was measured by gas chromatography and high-performance liquid chromatography, respectively; bacterial counts were obtained by plate count; gene expression was determined by RT-qPCR; and haemolytic activity was analyzed against goat erythrocytes. We found slightly but significantly lower final counts of Listeria and Bifidobacterium (p monocytogenes cells from cocultures than in those from monocultures. In contrast, the hly and luxS genes, which code for the cytolysin listeriolysin O and participate in biofilm formation, respectively, were overexpressed when L. monocytogenes was grown in coculture. This indicates that the presence of Bifidobacterium is able to modify the gene expression and haemolytic activity of L. monocytogenes when both microorganisms grow together.

  15. O impacto de uma psicoterapia breve pais-bebê para as representações acerca da maternidade no contexto da depressão

    Directory of Open Access Journals (Sweden)

    Schwengber, Daniela Delias de Sousa

    2009-01-01

    Full Text Available O presente estudo investigou as mudanças nas representações acerca da maternidade em uma mãe com indicadores de depressão ao longo de uma psicoterapia breve pais-bebê. Foi utilizado um delineamento de estudo de caso único, sendo que as representações maternas foram examinadas em três momentos: antes, durante e após a psicoterapia. Os relatos foram analisados a partir de quatro eixos interpretativos que constituem a constelação da maternidade de Stern (1997 vida-crescimento; relacionar-se primário; matriz de apoio; e reorganização da identidade. Os resultados revelaram que as mudanças nas representações da mãe acerca do relacionamento com a sua própria mãe desempenharam um papel central na reelaboração de esquemas a respeito de si mesma, do bebê e do relacionamento conjugal. Aponta-se para a efetividade da utilização dos temas da constelação da maternidade como eixos interpretativos na avaliação de processo psicoterápico envolvendo pais e bebê

  16. DISERTACIÓN SOBRE JUSTICIA Y EQUIDAD

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    Sayuri P. Tamura M.

    2001-01-01

    Full Text Available «Disertación sobre equidad y justicia» es básicamente una respuesta a una inquietud en particular que yo tenía sobre las distintas posiciones que existen sobre estos dos temas y cómo definir cuál es la más acertada. Hice una descripción del punto de vista de Amartya Sen –la violación de los derechos sobre la propiedad privada es justificable si evitan las hambrunas2– frente al de Robert Nozick –la propiedad privada es un derecho fundamental y como tal debe ser respetado3–, sin embargo, aunque ambos enfoques son totalmente contrarios tienen mucho sentido, por lo cual es bastante difícil decidirse por uno en particular. Fue de esta forma, enfrentando sus opiniones, que yo logré desarrollar una posición frente a estas dos perspectivas, y así dar mi opinión al DISERTACIÓN SOBRE JUSTICIA Y EQUIDAD SAYURI P. TAMURA M. Estudiante de Economía y Negocios Internacionales de la Universidad ICESI. respecto. Al final, llegué a una conclusión, que aunque no es la más original y tampoco es la única, tiene mucho sentido y seguramente de ser posible, la situación del país podría cambiar para bien.

  17. Costo-efectividad de una intervención breve de apoyo a mujeres indígenas de Hidalgo, México, que viven con consumidores excesivos de alcohol

    Directory of Open Access Journals (Sweden)

    Guillermina Natera Rey

    Full Text Available RESUMEN Objetivo Estimar la razón de costo-efectividad de una intervención breve de cinco pasos dirigida a reducir el estrés y los síntomas depresivos causados por convivir con un consumidor excesivo de alcohol. Métodos El análisis costo-efectividad se realizó usando un árbol de decisión y se basó en los síntomas depresivos medidos con la escala CES-D. La efectividad de la intervención breve se evaluó comparando un grupo de mujeres indígenas que recibieron la intervención (n = 43 con otro similar sin intervención (n = 30. Los grupos se evaluaron antes, inmediatamente después de la intervención y a los 12 meses y se llevó a cabo en el Estado de Hidalgo, en México. El tratamiento farmacológico fue seleccionado como alternativa de comparación utilizando distintas estimaciones. Para evaluar el valor de la remisión de los síntomas de depresión se emplearon las razones de cos-to-efectividad y costo-efectividad incremental. Asimismo, se realizaron análisis de sensibilidad. Resultados La intervención breve resultó ser más efectiva que la farmacológica en la remisión de síntomas depresivos y su costo fue más bajo ($US 31,24 y $US 107,60, respectivamente. Solo si la intervención breve incrementara su costo por encima de 338% o si su efectividad fuera menor de 22,8% dejaría de ser costo-efectiva. Conclusiones La intervención breve de cinco pasos es una alternativa costo-efectiva con la cual se consiguen reducir significativamente los síntomas de depresión de las mujeres indígenas ocasionados por convivir con un consumidor excesivo de alcohol, así como una opción accesible para la atención de la salud mental en el primer nivel de atención.

  18. Giacomo Leopardi y el viaje hacia la nada. Una breve introducción filosófica

    Directory of Open Access Journals (Sweden)

    Francesco Consiglio

    2014-12-01

    Full Text Available [ES] Giacomo Leopardi, poeta y filósofo italiano de los inicios del siglo XIX, elabora una imagen del mundo que une nihilismo y titanismo para contestar a la pregunta fundamental sobre el sentido de la existencia. Un interrogante, esto, al mismo tiempo metafísico, ético y estético. En el mundo materialista de Leopardi, ya no hay un sentido trascendente de la existencia y todo lo que existe se explica en los términos de su propia existencia. La dimensión ética adquiere matices ontológicos y, como en el universo de Anaximandro, la continua repetición de formas efímeras se acompaña a una suerte de pecado ontológico que conlleva la individuación: la culpa no tiene ningún sentido trascendente, sino está implicada por el simple hecho de venir al mundo, por la misma existencia. Con la indiferencia de estas dinámicas choca el sistema de valores del ser humano, que pierde sus coordenadas jerárquicas y el propio sentido de su existencia, hundido en la nada de un ser ya sin porqué, aplastado por el peso de las mecánicas de un mundo sin dios, donde ni siquiera la belleza del arte redime. Surge de aquí un profundo sentido de lo trágico al que la única respuesta puede ser un gesto titánico, un desafío rebelde. [EN] Giacomo Leopardi, Italian poet and philosopher of the early 19th century, elaborated an image of the world blending nihilism and titanism to respond to the fundamental question on the meaning of the existence. A philosophical doubt expressing at the same time both a metaphysical, ethical and esthetical concern. In Leopardi’s materialist world, there is no more transcendent sense of the existence and every being is explained in terms of its own existence. The ethical dimension acquires ontological shades and, like in Anaximander’s universe, the continuous repetition of ephemeral forms entail a kind of ontological sin deriving from individuation: fault has not transcendent sense, while it is implied by the same birth, by

  19. Impuesto sobre las transacciones financieras: Justicia fiscal

    OpenAIRE

    Mariño, José Ramón

    2016-01-01

    7 p.[Variante del título de la publicación : Boletín electrónico de Hegoa] [ESP] El Impuesto sobre las transacciones financieras se basa en establecer un gravamen sobre las operaciones financieras con el fin de estabilizar los mercados como consecuencia de la disminución de la especulación a corto plazo. Además es una fuente de financiación de inversiones socialmente deseables. Diez países de la Unión Europea han expresado su deseo de introducir el ITF, sin emba...

  20. Breves consideraciones acerca de la importancia de los árboles de decisión en el análisis de carteras

    Directory of Open Access Journals (Sweden)

    Julio César Riascos Hermoza

    2016-01-01

    Full Text Available La cada vez más estrecha relación entre las ciencias y/o disciplinas no es ajena a la relación derivada en los últimos 50 años entre las nanzas, la Econometría, la modelación estadística y los procesos de simulación computacional. El propósito de este artículo introductorio reside en exponer algunas breves consideraciones con respecto al potencial manejo de los árboles de decisión en la teoría nanciera y particularmente como complemento al diseño de los tradicionales árboles de rentabilidad. El documento sugiere que la importancia atribuida a los árboles de rentabilidad así como el modelamiento de carteras propios de la teoría financiera debe ser complementada y avanzada por herramientas de simulación cuya aplicación posibilita anticipar situaciones futuras y elaborar políticas ex-ante. De acuerdo con lo anterior, se refuerzan los análisis de un portafolio de inversión a través de árboles de decisión bajo tres escenarios que comprenden diferentes combinaciones de riesgo y rentabilidad. Mediante este ejercicio no complejo, se concluye que las rentabilidades de una cartera de inversión asociadas a los escenarios planteados arrojan resultados diversos que dependen de la probabilidad estadística de ganar o de perder.

  1. ESTEREÓTIPOS ÉTNICOS E REPRESENTAÇÕES SOCIAIS: uma breve incursão teórica

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    Karla Cristina Silva Sousa

    2015-03-01

    Full Text Available O presente artigo é fruto das discussões realizadas na dissertação de Mestrado em Educação cujo título foi “Estereótipos étnicos nas representações de crianças escolarizadas em São Luís do Maranhão” apresentada ao Programa de Pós-Graduação em Educação da Universidade Federal do Maranhão. O objetivo do artigo é situar a relação existente entre estereótipos étnicos e representações sociais, fazendo uma breve apresentação destes a partir das ideias de Jodelet, Moscovici, Tajfel, Batista, Fernandes, Bastide. Concluimos que existe uma estreita relação entre estereótipos e representações sociais. Palavras-chave: Estereótipos. Representações sociais. Preconceito. ABSTRAC This article is a result from discussions made in dissertation entitled “Ethnical stereotypes in the representations of children in school age in São Luís, Maranhão”, presented to the Masters in Education Program of Federal University of Maranhão. The purpose of the article is set the relation between ethnical stereotypes and social representations, by doing a brief presentation of them from Jodelet, Moscovivi, Tajfel, Batista, Fernandes and Bastide ideas. In conclusion I argue that exist a thin relationship between stereotypes and social epresentations. Keywords: Stereotypes. Social representations. Prejudice.

  2. UM ESTUDO SOBRE A OBRA “FUNDAMENTOS DA POLÍTICA JURÍDICA” DE OSVALDO FERREIRA DE MELO, ATRAVÉS DOS ELEMENTOS DE PERCEPÇÃO JURÍDICA DA “TEORIA TRIDIMENSIONAL DO DIREITO” DE MIGUEL REALE

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    Lehmkuhl, Mílard Zhaf Alves

    2012-01-01

    O presente trabalho apresenta uma visão da obra “Fundamentos da Política Jurídica” de Osvaldo Ferreira de Melo a partir dos elementos de Percepção Jurídica, fato – valor – norma, colhidos da obra “Teoria Tridimensional do Direito” de Miguel Reale. O artigo se inicia com uma breve apresentação da obra “Fundamentos da Política Jurídica” e sobre os elementos de percepção jurídica da “Teoria Tridimensional do Direito”. Partindo dessa contextualização e dessa tridimensionalidade como ferramenta de...

  3. Distribuição etária do sarampo e vacinação: considerações sobre alguns dados do município do Rio de Janeiro

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    Paulo C.R. Barata

    1985-03-01

    Full Text Available São apresentados alguns dados sobre a distribuição etária dos casos notificados de sarampo no Município do Rio de Janeiro entre 1964 e 1982, observando-se a modificação ocorrida nesta distribuição entre o período pré-vacinação e o período pós-vacinação. Estes dados são comparados com dados de alguns outros países e é feita uma breve discussão sobre a influência da vacinação na epidemiologia do sarampo.Some data are presented on the age distribution of the reported cases of measles in the Município do Rio de Janeiro, Brazil, between 1964 and 1982, focusing on changes in this distribution between the prevaccination and postvaccination years. These data are compared with those of some other countries and a short discussion is made about the influence of vaccination on the epidemiology of measles.

  4. A Companhia de Jesus e o Breve de 1639: o propósito e o acontecimento * The Society of Jesus and the Bull of 1639: their main intentions and the following events

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    EUNÍCIA BARROS BARCELOS FERNANDES

    2014-09-01

    Full Text Available Resumo: O artigo analisa diferentes condições na relação entre jesuítas, colonos e indígenas; apresentando matrizes de ideário e experiência que geraram o Breve de 1639, que declarava liberdade irrestrita dos indígenas, bem como sua recepção no Rio de Janeiro. A observação da mudança de contextos e de relações entre os agentes expõe a singularidade da percepção de liberdade indígena contida no Breve e como tal singularidade atingia anseios de colonos.Palavras-chave: Breve de 1639 – Companhia de Jesus – Colonos – Indígenas – Liberdade – Rio de Janeiro. Abstract: This work analyses the different perspectives in the relationship of Jesuits, settlers and amerindians; by that we present some of the arrays of the ideas and the experiences that lead to the Bull of 1639 and also the reception of this document, which declared freedom without further conditions to all amerindians, in Rio de Janeiro. The opportunity to observe the changes in the contexts and in the relationships allow us to expose singularities in the perception of liberty defended to the amerindians presented to us by the Bull and how these particularities affected lives and wishes of the settlers in Rio de Janeiro. Keywords: Bull of 1639 – Society of Jesus – Settlers – Amerindians – Liberty – Rio de Janeiro.

  5. Influência das características estruturais sobre a processabilidade do polibutadieno alto cis Influence of structural characteristics on the high-cis polybutadiene processability

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    Ivana L. Mello

    2004-01-01

    Full Text Available Características físicas, tais como peso molecular, distribuição de peso molecular e ramificações, têm importância significativa na processabilidade do polibutadieno. Assim, deve haver um balanceamento dessas características a fim de se obter boas propriedades mecânicas e boa processabilidade. Este Trabalho apresenta uma breve revisão sobre a influência das características estruturais na processabilidade do polibutadieno produzido por diferentes sistemas catalíticos. São apresentadas também algumas técnicas encontradas na literatura para melhorar a processabilidade desse elastômero.Physical characteristics, such as, molecular weight, molecular weight distribution and branching, have a significant influence on polybutadiene processability. Thus, a balance should exist in these characteristics for good mechanical properties and processability. This work brings a brief review of the influence of structural characteristics on the processability of polybutadiene produced by different catalyst systems. Also presented are some techniques from the literature aimed at improving processability of this elastomer.

  6. Lugar de dudas. Sobre la práctica del análisis histórico. Breviario de inseguridades, de R. Silva

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    Alejandro Sánchez Lopera

    2017-01-01

    Full Text Available Esta obra del historiador colombiano Renán Silva (1951 es una polémica en torno a debates perennes en el campo historiográfico. Ofrece una notable reflexión sobre la construcción autónoma de problemas y objetos de investigación, evitando la tentación de corroborar aquello que sabemos previamente (nuestro prejuicio en el material de campo. Las propuestas de Pierre Bourdieu, Norbert Elias y Michel de Certeau operan en el libro como precaución frente a la tentación empirista de que el objeto de investigación existe dado en la realidad, de forma espontánea —sin reconocer que el objeto es un recorte, una creación (rigurosa o no del investigador—. Dividido en seis breves capítulos, el libro toma distancia de los dualismos convencionales que moralizan el análisis (élites vs. masas, criticando el empirismo y la pobreza conceptual de la historiografía reciente (p. 35. Revisa, a su vez, los dilemas éticos y epistemológicos a los que se enfrenta la práctica histórica universitaria: el anacronismo, el eurocentrismo-etnocentrismo, la subordinación (periferia, subalternidad, colonialismo.

  7. Considerações sobre a aprendizagem da performance musical

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    Cerqueira,Daniel Lemos; Zorzal,Ricieri Carlini; Ávila,Guilherme Augusto de

    2012-01-01

    Este artigo oferece uma proposta para fundamentação da prática musical de instrumentos e canto, enfatizando procedimentos de estudo, baseando-se principalmente na Teoria da Aprendizagem Pianística de José Alberto Kaplan. Paralelamente, foi realizada uma breve releitura crítica da história do ensino de instrumentos musicais e métodos para educação musical, em diálogo com áreas afins à Performance Musical, entre elas Psicologia Cognitiva, Neurociência e Educação Física.

  8. Víctimas de accidentes de tránsito. Su protección en el mundo a través del aseguramiento obligatorio y breve referencia al sistema implantado en Colombia

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    Navas Herrera, María Fernanda; Pontificia Universidad Javeriana

    2009-01-01

    El presente artículo traduce en breves comentarios, una amplia muestra estadísticade los diversos sistemas de aseguramiento de las víctimas en accidentes detránsito en diferentes países del mundo. Basándose en una recopilación de datosrealizada por la Asociación Internacional de Derecho de Seguros (AIDA) durantelos últimos 10 años a nivel mundial, este escrito muestra las diferentes coberturasdel seguro obligatorio de responsabilidad civil de automóviles, las tarifas, loslímites de indemnizac...

  9. Dos trilhos aos palacetes: refletindo sobre o acervo arquitetônico da Av. Rio Branco, Santa Maria, RS

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    Maria Dinair Machado

    2015-12-01

    Full Text Available Este artigo propõe uma análise sobre a preservação e conservação do patrimônio cultural edificado no centro do município de Santa Maria, mais precisamente o patrimônio localizado na Avenida Rio Branco, na área central da cidade. O município possui sua história vinculada à ferrovia que se pode observar no patrimônio cultural edificado,  sua riqueza e diversidade, localizado no centro urbano. O nosso recorte espacial é focado na antiga Avenida Progresso, atual Avenida Rio Branco, eixo que ligava a estação férrea a Praça Saldanha Marinho e a Rua do Acampamento na área central. Para este trabalho foi  realizada  pesquisa  exploratória qualitativa, utilizando-se de observação por meio de registros fotográficos do centro histórico de Santa Maria, delimitando-se à  Avenida Rio Branco. No trabalho consta um breve histórico desta avenida, uma descrição dos prédios de valor histórico e as ações realizadas ao longo dos últimos anos. Nas conclusões, é proposta uma possibilidade de intervenção territorial a partir das necessidades como políticas públicas de preservação decorrentes do atual estado de abandono das edificações naquele local.

  10. XII ASSEMBLEIA GERAL ORDINÁRIA DO SÍNODO DOS BISPOS SOBRE A PALAVRA DE DEUS

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    Johan Konings

    2009-01-01

    Full Text Available Em outubro de 2008 transcorreu em Roma a XII Assembleia Geral Ordin ária do Sínodo dos Bispos, o qual é uma instituição permanente criada pelo Concílio Vaticano II para manter o diálogo dos pastores-bispos das Igrejas particulares. Nesta Assembleia, dedicada à Palavra de Deus na vida e na missão da Igreja, foi retomada praticamente a Constituição Dogmática Dei Verbum do Vaticano II. Depois de apresentar o tema da Palavra de Deus, traçamos um breve histórico focalizando os inícios da Tradição cristã e os tempos modernos desde Leão XIII até hoje. Em seguida descrevemos a XII Assembleia Geral, especialmente a Relatio post Disceptationem e as Propositiones. Concluímos com uma reflexão teológicopastoral sobre a Revelação e a leitura bíblica, e sugestões para a prática. ABSTRACT: In October of 2008 the XII Ordinary General Assembly of the Sinod of Bishops took place in Rome. The Sinod of Bishops is a permanent institution created by the II Vatican Council in order to maintain the dialogue of pastorsbishops of the particular Churches. This Assembly, dedicated to the Word of God in the life and in the mission of the Church, basically treated again the Dogmatic Constitution Dei Verbum of the II Vatican Council. After presenting the theme of the Word of God, we outline a brief history focusing on the beginnings of the Christian Tradition and the modern epoch since Leo XIII until today. Next we describe the XII General Assembly, especially the Relatio post Disceptationem and the Propostiones. We conclude with a theological-pastoral reflection on Revelation and the reading of the bible, and some practical suggestions.

  11. Um plano sobre o caos (reflexão acerca do evento A plan on chaos (a reflection on event

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    Ana Lúcia M. de Oliveira

    1995-02-01

    Full Text Available Inspirado no método das investigações wittgensteinianas, este ensaio parte de um breve estudo sobre a forma do fragmento para chegar a seu foco central: um questionamento da categoria de evento. Em síntese, intenta-se uma abordagem estética de um problema situado em um ponto de cruzamento entre a teoria da literatura, a história, a filosofia e até mesmo a ciência. A liga possível é sugerida por Deleuze e Guattari, em sua análise das três grandes formas de pensamento - a arte, a ciência e a filosofia - como diferentes maneiras de enfrentar o caos, traçando um plano secante que o atravessa. Como se trata de uma pesquisa ainda em curso, o que se pretende é o levantamento de questões básicas, apontando para os possíveis desdobramentos por vir.Inspired by Wittgensteinian philosophical investigation, this essay begins with an analysis of fragmentary form in order to reach the central point: a discussion on event as a category. Briefly, it seeks an aesthetic approach to a problem situated at the crossroads of literary theory, history, philosophy, and even science. A possible link is suggested by Deleuze and Guattari in their discussion of the three major trends of thought - art, science, and philosophy- as different ways of dealing with chaos, tracing a plan which traverses it. As a study still in progress, the essay is intended to raise basic questions to be developed further through future investigation.

  12. Investigación educativa reciente sobre México

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    Núñez-DelaPeña, Francisco J.; Mejía-Arauz, Rebeca

    1985-01-01

    Se presenta una muestra de la investigación educativa sobre México: 22 disertaciones doctorales aceptadas en universidades norteamericanas entre 1982 y 1984. Dos estudios conciernen a la teoría del capital humano; cinco tratan diversos aspectos relacionados con la administración educativa; uno es sobre la televisión; otro sobre las habilidades fonéticas de los niños; uno más es sobre profesionalización de los médicos; cuatro tocan aspectos de política educativa; tres tratan sobre los maestros...

  13. Un discurso de Ramón y Cajal sobre El Quijote

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    González Quirós, José Luis

    2006-04-01

    Full Text Available The article is a brief study to a lecture by Santiago Ramon y Cajal, entitled Psichology of Don Quixote and Quixotism. It is a rather unknown text, as well as one of the main works regarding the topic produced at the time of the 3rd centennary of Don Quixote. The lecture was commissioned by the Medical College of San Carlos, and was read on May 9th, 1905. The existing editions contain quite a few printing errors. Our analysis sets out Cajal’s ideas regarding the situation of Spanish science and the necessary measures that need to be taken. We also put these ideas in comparison with those of Unamuno and Ortega, the two main Spanish authors to write about the same topic in the same years. We emphasize the agreement between Unamuno and Cajal, as well as the radical difference we can find between Ortega’s and Cajal’s approaches to the subject of science.El artículo es un breve análisis del discurso de Santiago Ramón y Cajal titulado Psicología de Don Quijote y el quijotismo, un texto poco conocido que fue una de las piezas principales producida en torno al tema con motivo del III centenario del Quijote. El discurso de Ramón y Cajal le fue encargado por el Colegio Médico de San Carlos, y se pronunció en Madrid el día 9 de Mayo de 1905. Las ediciones existentes contienen algunas erratas e inexactitudes. Nuestro análisis expone las ideas de Ramón y Cajal en relación con la situación de la ciencia española y con los remedios que es necesario poner en práctica y con el espíritu que debe animarlos para poner luego en relación las ideas de Ramón y Cajal con las de Unamuno y Ortega, los dos principales autores españoles que escribieron sobre el tema en aquellos mismos años, subrayando la coincidencia entre Unamuno y Ramón y Cajal y la llamativa distancia de Ortega con las ideas de nuestro histólogo en relación con la ciencia.

  14. Pórticos, letreiros, lareiras Le Corbusier e Robert Venturi, sobre simbolismo e velocidade no modernismo

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    Rafael Urano Frajndlich

    2010-12-01

    Full Text Available Este artigo procura investigar a leitura que o arquiteto Robert Venturi faz de alguns traços da obra de Le Corbusier, para conceber suas considerações sobre arquitetura e comunicação. Para isso, foram estudadas, de modo breve, as maneiras como Le Corbusier já fazia uso, em sua obra, de algumas categorias que, posteriormente, seriam atribuídas, excelentemente, aos ditos pós-modernos: a retórica, a alusão, a memória dos centro históricos e dos interiores decorados. Tendo em vista a obra geral do arquiteto suíço, é certo que essas categorias aparecem somente nas entrelinhas de seu projeto modernista de reorganização das cidades. Entretanto, uma leitura de minúcias revela alguns temas – especificamente o interesse de Corbusier nos monumentos de Paris, seus pórticos desenhados na Ville contemporaine (1921, e a lareira com motivos surrealistas na cobertura De Beistegui (1929 que seriam objeto de pleno interesse para historiadores e arquitetos da década de 1960. Justamente, nessa recuperação de traços obscuros de Corbusier, Venturi posiciona alguns de seus temas, especificamente em suas soluções residenciais, privilegiando – e, em alguns casos, mesmo exagerando, a imagem chaminé, duplicando sua altura ou colocando-a como grande ordenadora da planta. Todo esse debate será estudado tendo, como pano de fundo, escritos recentes que realizam um balanço crítico das rupturas engendradas pela dita arquitetura pós-moderna com os modernismos. Fazendo uso de considerações de Andreas Huyssen, Paul Virilio e Beatriz Colomina, entre outros, procurou-se ora delimitar, com maior clareza, a fronteira entre essas duas correntes do século 20, ora diluir seus contornos, revelando semelhanças que, eventualmente, fazem-nas indissociáveis.

  15. Sobre una Rodoficea parasita de Gelidiaceas

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    Seoane Camba, Juan Antonio

    1982-01-01

    En este trabajo se hace un estudio de una Rodofícea parásita, encontrada sobre Gelidium cantabricum Seoane-Camba y Gelidium sesquipedale (Clemente) Thuret. Por sus características generales atribuímos esta planta al género Gelidiocolax. descrito por Gardner en 1927 para un parásito de Gelidium pulchrum Gardner de las costas americanas del Pacífico...

  16. Reflexiones sobre el concepto de justicia

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    Víctor C. Pascual Planchuelo

    2005-06-01

    Full Text Available Este ensayo constituye una reflexión sobre el concepto de Justicia. No se centra, como tantos estudios al respecto, en el plano teórico y metafísico, sino que atiende problemas de mayor actualidad: la opinión de los ciudadanos, la postura de los profesionales y otros asuntos controvertidos del ámbito del Derecho.

  17. Sobre a dor e a delícia da atuação Psicológica no SUAS

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    Alexandre Franca Barreto

    Full Text Available Este artigo traz reflexões sobre o trabalho do psicólogo no Sistema Único de Assistência Social, com base em uma experiência de seis anos de atuação no campo e em pesquisa em serviços de execução de medidas socioeducativas e educação social de rua, nos Municípios de Olinda e Recife, PE. Após breve histórico de minha trajetória no SUAS, procuro contextualizar minha identidade no atual cenário éticopolítico da profissão, envolvido pelas transformações no contexto da formação e pelas tendências atuais nos cenários da prática psicológica em políticas públicas. Proponho alguns elementos norteadores do exercício profissional na lógica do pêndulo: o cuidar e ser cuidado, a agressividade e a passividade, escutar o Outro e falar de si, como polos por onde devemos transitar para desenvolver uma prática qualificada e criativa. Por fim, são apontados questionamentos sobre a dinâmica do psicólogo nos serviços de assistência social, com algumas recomendações para o suporte e a qualificação do trabalho.

  18. QUALIDADE DO AR – PARÂMETROS DE CONTROLE E EFEITOS NA SAÚDE HUMANA: UMA BREVE REVISÃO

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    Antônio Hermeson Castro

    2013-11-01

    Full Text Available Estudos relacionados à poluição atmosférica e a qualidade do ar crescem a cada ano. Em geral as pesquisas desenvolvidas envolvem o conhecimento sobre os componentes nocivos a saúde presentes no meio ambiente. De acordo com a legislação brasileira os poluentes de maior importância monitorados são representados pelo monóxido de carbono (CO, os óxidos de nitrogênio (NOx, o dióxido de enxofre (SO2, o ozônio (O3, a fumaça e os materiais particulados: partículas inaláveis (PM10 e PM2,5 e partículas totais em suspensão (PTS. Outras substâncias como os compostos orgânicos BTEX (benzeno, tolueno, etilbenzeno e xilenos também foram investigados devido à elevada abundancia em atmosferas urbanas. Em particular, este artigo apresenta uma revisão sobre os principais poluentes atmosféricos, fontes de emissão, ocorrência, legislação e efeitos sobre a saúde humana.

  19. Relatório brasileiro sobre desenvolvimento social

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    Luiz Felipe Lampreia

    1995-08-01

    Full Text Available O relatório nacional brasileiro, elaborado como parte do processo de preparação da participação brasileira na Cúpula Mundial para o Desenvolvimento Social, busca traçar um quadro da situação do Brasil em relação aos três temas centrais do evento: alívio e redução da pobreza, expansão do emprego produtivo e integração social. Com o objetivo de qualificar a peculiaridade dos desafios sociais com os quais o Brasil se defronta, a primeira parte consiste de um quadro abrangente, onde estão expostos os dados gerais mais relevantes. A segunda parte, de caráter conceituai, explora a relação à dicotomia desenvolvimento econômico/desenvolvimento social, políticas econômicas/políticas sociais. Passa-se, então, à parte do relatório mais diretamente ligada aos temas centrais da Cúpula. O capítulo sobre pobreza busca sistematizar algumas das principais características desse fenômeno no Brasil; em "Emprego e mercado de trabalho" examinam-se questões como a baixa taxa de desemprego aberto do mercado brasileiro e sua relação com a má qualidade dos postos de trabalho existentes; finalmente, "Integração social e políticas sociais" salienta a necessidade de novas formas de solidariedade social, o que implicaria a reformulação da lógica de financiamento das políticas sociais e sua integração num conjunto coeso, ao lado de novas parcerias do Estado com a sociedade e do setor público com o setor privado. À guisa de conclusão, alinham-se as principais diretrizes do Governo Federal para o período 1995-1999. Depois de enfatizar a necessidade de que quatro condições prévias - fortalecimento da democracia, manutenção da estabilidade econômica, recuperação do crescimento sustentado e reforma do Estado - sejam alcançadas, identificam-se cinco metas prioritárias na área social: agricultura, educação, emprego, saúde e segurança. O relatório termina com breve esboço da estrutura do Programa Comunidade Solid

  20. Los campeonatos escolares en España: breve síntesis histórica = School Championships in Spain: a brief historical summary

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    Antonio Rivero Herráiz

    2013-03-01

    Full Text Available El objetivo de este trabajo es realizar un breve análisis histórico de los campeonatos deportivos escolares en España desde sus inicios en el año 1948, así como de su posterior evolución en etapas más recientes. En este sentido, dos han sido los factores que han tenido una influencia decisiva en el desarrollo y en la organización del los campeonatos deportivos escolares en España: las leyes reguladoras de la Educación Física y la disyuntiva entre la organización de un deporte para escolares o de un deporte para niños en edad escolar. De este estudio también se desprende que la implantación de un buen plan de infraestructuras deportivas resulta imprescindible para el adecuado desarrollo de la actividad y, al mismo tiempo, que la implicación de los centros escolares es la más eficaz estrategia para alcanzar los objetivos deseados de motivación y participación de niños y jóvenes.-------------------------------------------------------------------------------The objective of this work is to realise a brief historical analysis of the scholastic sport championships in Spain from its beginnings in 1948, as well as of their later evolution in more recent stages. In this sense, two have been the factors that have had a decisive influence in the development and the organization of the scholastic sport championships in Spain: the regulating laws of the Physical education and the dilemma between the organization of a sport for students or a sport for children in scholastic age. This study also it is come off that the implantation of a good sport facilities plan is essential for the suitable development of the activity and, at the same time, that the implication of the scholastic centres is the most effective strategy to reach the wished objectives of motivation and participation of children and young people.

  1. Two β-galactosidases from the human isolate Bifidobacterium breve DSM 20213: molecular cloning and expression, biochemical characterization and synthesis of galacto-oligosaccharides.

    Directory of Open Access Journals (Sweden)

    Sheryl Lozel Arreola

    Full Text Available Two β-galactosidases, β-gal I and β-gal II, from Bifidobacterium breve DSM 20213, which was isolated from the intestine of an infant, were overexpressed in Escherichia coli with co-expression of the chaperones GroEL/GroES, purified to electrophoretic homogeneity and biochemically characterized. Both β-gal I and β-gal II belong to glycoside hydrolase family 2 and are homodimers with native molecular masses of 220 and 211 kDa, respectively. The optimum pH and temperature for hydrolysis of the two substrates o-nitrophenyl-β-D-galactopyranoside (oNPG and lactose were determined at pH 7.0 and 50°C for β-gal I, and at pH 6.5 and 55°C for β-gal II, respectively. The kcat/Km values for oNPG and lactose hydrolysis are 722 and 7.4 mM-1s-1 for β-gal I, and 543 and 25 mM-1s-1 for β-gal II. Both β-gal I and β-gal II are only moderately inhibited by their reaction products D-galactose and D-glucose. Both enzymes were found to be very well suited for the production of galacto-oligosaccharides with total GOS yields of 33% and 44% of total sugars obtained with β-gal I and β-gal II, respectively. The predominant transgalactosylation products are β-D-Galp-(1→6-D-Glc (allolactose and β-D-Galp-(1→3-D-Lac, accounting together for more than 75% and 65% of the GOS formed by transgalactosylation by β-gal I and β-gal II, respectively, indicating that both enzymes have a propensity to synthesize β-(1→6 and β-(1→3-linked GOS. The resulting GOS mixtures contained relatively high fractions of allolactose, which results from the fact that glucose is a far better acceptor for galactosyl transfer than galactose and lactose, and intramolecular transgalactosylation contributes significantly to the formation of this disaccharide.

  2. Two β-galactosidases from the human isolate Bifidobacterium breve DSM 20213: molecular cloning and expression, biochemical characterization and synthesis of galacto-oligosaccharides.

    Science.gov (United States)

    Arreola, Sheryl Lozel; Intanon, Montira; Suljic, Jasmina; Kittl, Roman; Pham, Ngoc Hung; Kosma, Paul; Haltrich, Dietmar; Nguyen, Thu-Ha

    2014-01-01

    Two β-galactosidases, β-gal I and β-gal II, from Bifidobacterium breve DSM 20213, which was isolated from the intestine of an infant, were overexpressed in Escherichia coli with co-expression of the chaperones GroEL/GroES, purified to electrophoretic homogeneity and biochemically characterized. Both β-gal I and β-gal II belong to glycoside hydrolase family 2 and are homodimers with native molecular masses of 220 and 211 kDa, respectively. The optimum pH and temperature for hydrolysis of the two substrates o-nitrophenyl-β-D-galactopyranoside (oNPG) and lactose were determined at pH 7.0 and 50°C for β-gal I, and at pH 6.5 and 55°C for β-gal II, respectively. The kcat/Km values for oNPG and lactose hydrolysis are 722 and 7.4 mM-1s-1 for β-gal I, and 543 and 25 mM-1s-1 for β-gal II. Both β-gal I and β-gal II are only moderately inhibited by their reaction products D-galactose and D-glucose. Both enzymes were found to be very well suited for the production of galacto-oligosaccharides with total GOS yields of 33% and 44% of total sugars obtained with β-gal I and β-gal II, respectively. The predominant transgalactosylation products are β-D-Galp-(1→6)-D-Glc (allolactose) and β-D-Galp-(1→3)-D-Lac, accounting together for more than 75% and 65% of the GOS formed by transgalactosylation by β-gal I and β-gal II, respectively, indicating that both enzymes have a propensity to synthesize β-(1→6) and β-(1→3)-linked GOS. The resulting GOS mixtures contained relatively high fractions of allolactose, which results from the fact that glucose is a far better acceptor for galactosyl transfer than galactose and lactose, and intramolecular transgalactosylation contributes significantly to the formation of this disaccharide.

  3. Utilizzo della metodologia di progettazione secondo ISO 9001:2000 per il re-engineering dei ricoveri chirurgici in degenza breve

    Directory of Open Access Journals (Sweden)

    G. Tarassi

    2003-05-01

    Full Text Available

    Obiettivi: migliorare l’assistenza al paziente, riducendo
    il periodo della degenza nella fase immediatamente
    postoperatoria; ridurre l’inappropriatezza di ricoveri in particolare inseriti nella lista del decreto sui LEA; utilizzare con maggior efficienza i posti letto e le risorse infermieristiche; avviare la organizzazione dell’attività per processi all’interno del Sistema qualità ISO 9001:2000.


    Materiali e metodi: la riorganizzazione ha interessato
    tutte le 6 unità operative di area chirurgica (chirurgia generale, chirurgia plastica, urologia, oculistica, ortopedia, ginecologia i servizi di prenotazione ed accettazione ricoveri, l’ambulatorio degli accertamenti preoperatori per un’area polidisciplinare di degenza breve da lunedì a venerdì con patologie chirurgiche con degenza media tra 0 e 4 giorni. Definiti i membri del gruppo di progetto, è
    stato stilato un calendario comprensivo di 2 riesami, 2 verifiche ed 1 validazione, secondo la metodologia ISO. È stato inoltre realizzato uno studio preliminare effettuato in collaborazione con tre ospedali.

    Risultati: risparmio di giornate di degenza a parità di case-mix; risparmio risorse infermieristiche a parità di assistenza; effettuazione DRG chirurgici a rischio di inappropriatezza in Day Surgery o One Day Surgery; verifica di un buon livello di customer satisfation; articolazione di unica segreteria per l’intero processo da prenotazione, accertamenti preoperatori, ricovero, intervento, dimissione, controlli con unico riferimento per il paziente con buon gradimento complessivo; costruzione di percorsi diagnostico-terapeutici
    condivisi tra diverse.

    Conclusioni: il progetto ha permesso di utilizzare in modo più efficiente posti letto e di organizzare per processi l’attività assistenziale con soddisfazione dell’utenza esterna e

  4. Administration of Bifidobacterium breve PS12929 and Lactobacillus salivarius PS12934, Two Strains Isolated from Human Milk, to Very Low and Extremely Low Birth Weight Preterm Infants: A Pilot Study

    Directory of Open Access Journals (Sweden)

    Laura Moles

    2015-01-01

    Full Text Available The preterm infant gut has been described as immature and colonized by an aberrant microbiota. Therefore, the use of probiotics is an attractive practice in hospitals to try to reduce morbidity and mortality in this population. The objective of this pilot study was to elucidate if administration of two probiotic strains isolated from human milk to preterm infants led to their presence in feces. In addition, the evolution of a wide spectrum of immunological compounds, including the inflammatory biomarker calprotectin, in both blood and fecal samples was also assessed. For this purpose, five preterm infants received two daily doses (~109 CFU of a 1 : 1 mixture of Bifidobacterium breve PS12929 and Lactobacillus salivarius PS12934. Bacterial growth was detected by culture-dependent techniques in all the fecal samples. The phylum Firmicutes dominated in nearly all fecal samples while L. salivarius PS12934 was detected in all the infants at numerous sample collection points and B. breve PS12929 appeared in five fecal samples. Finally, a noticeable decrease in the fecal calprotectin levels was observed along time.

  5. Administration of Bifidobacterium breve PS12929 and Lactobacillus salivarius PS12934, two strains isolated from human milk, to very low and extremely low birth weight preterm infants: a pilot study.

    Science.gov (United States)

    Moles, Laura; Escribano, Esperanza; de Andrés, Javier; Montes, María Teresa; Rodríguez, Juan M; Jiménez, Esther; Sáenz de Pipaón, Miguel; Espinosa-Martos, Irene

    2015-01-01

    The preterm infant gut has been described as immature and colonized by an aberrant microbiota. Therefore, the use of probiotics is an attractive practice in hospitals to try to reduce morbidity and mortality in this population. The objective of this pilot study was to elucidate if administration of two probiotic strains isolated from human milk to preterm infants led to their presence in feces. In addition, the evolution of a wide spectrum of immunological compounds, including the inflammatory biomarker calprotectin, in both blood and fecal samples was also assessed. For this purpose, five preterm infants received two daily doses (~10(9) CFU) of a 1:1 mixture of Bifidobacterium breve PS12929 and Lactobacillus salivarius PS12934. Bacterial growth was detected by culture-dependent techniques in all the fecal samples. The phylum Firmicutes dominated in nearly all fecal samples while L. salivarius PS12934 was detected in all the infants at numerous sample collection points and B. breve PS12929 appeared in five fecal samples. Finally, a noticeable decrease in the fecal calprotectin levels was observed along time.

  6. Efetividade da intervenção breve para o uso abusivo de álcool na atenção primária: revisão sistemática

    Directory of Open Access Journals (Sweden)

    Maria Odete Pereira

    2013-06-01

    Full Text Available A pesquisa identificou estudos clínicos acerca das intervenções breves para o uso abusivo de álcool e analisou a eficácia dos aconselhamentos para os consumidores. Estudo bibliográfico sistemático que utilizou artigos publicados no período de 1997 a 2010. Os achados mostraram que a estratégia de intervenção breve é efetiva na redução da frequência e quantidade de álcool, com melhores resultados quando aplicada na atenção primária. Todos os profissionais da área da saúde obtêm bons resultados com o manejo dessa terapia. Não foi possível definir se a estratégia é mais eficaz se aplicada em bebedores de uso nocivo ou em crônicos, uma vez que a intervenção depende da mobilização de recursos motivacionais do paciente e de mudanças de atitude relacionadas ao consumo de álcool.

  7. Análise de Teoria de Resposta ao Item de um instrumento breve de avaliação de comportamentos antissociais = Item Response Theory Analysis of a brief instrument for assessing antisocial behaviors

    Directory of Open Access Journals (Sweden)

    Hauck Filho, Nelson

    2014-01-01

    Full Text Available Comportamentos antissociais são comuns a diversas condições psicopatológicas, incluindo transtornos da personalidade (e. g. , antissocial e narcisista e transtornos do humor (e. g. , transtorno bipolar. Todavia, até o momento, havia uma importante lacuna no contexto brasileiro no que diz respeito à avaliação breve dos comportamentos antissociais em indivíduos adultos de contextos não carcerários. Em virtude disso, o presente estudo teve como objetivo a construção e a análise mediante Teoria de Resposta ao Item de um instrumento breve para uso em pesquisas e rastreio junto à população geral adulta. As análises das respostas de 204 estudantes universitários (média de idades = 23,56 anos; DP = 7,70; 60,6% mulheres a um conjunto de itens permitiram reter 13 itens com excelentes propriedades psicométricas. Esses itens se mostraram avaliativos de um fator geral de antissocialidade, interpretável como uma propensão ao antagonismo, à não cooperação e à agressão em uma diversidade de contextos sociais. Limitações do estudo são discutidas ao final

  8. Análisis de las formulaciones sobre la resistencia a cortante del hormigón

    Directory of Open Access Journals (Sweden)

    Freire Tellado, Manuel J.

    2005-06-01

    Full Text Available In this paper, we analyse all of the formulations stated in the Spanish regulation, which allows to evaluate concrete contribution to a structure shear strength. We also offer a historical review of the different formulations included not only in the Spanish regulation, but also in the current versions of other relevant guidelines; this opens a short discussion on the problem to be considered. Afterwards, we contrast results obtained under the Spanish regulation with those obtained under other rules. A numerical analysis is done parallely on two rectangular sections, one plane (axb and the other being edgeways (bxa. Both have the same dimensions and are under the action of the same bending moment. Finally, the formulations previously stated are applied to a floor concrete wrought; we study different types of resistance on the concrete with three armed solutions for each case, we compare results obtained, and this leads to some curious conclusions.El artículo realiza un análisis de la expresiones de la normativa española que permiten estimar la contribución del hormigón en la resistencia a cortante de una estructura, sugiriendo algunas precisiones, mejoras y refinamientos posibles. Seguidamente se realiza un repaso de las diferentes expresiones que planteó la normativa española a lo largo de su historia y de las versiones actuales de otras normas de reconocido prestigio, realizándose una breve discusión sobre el problema del ancho a considerar. Posteriormente se contrastan los resultados de las formulaciones españolas con los que derivan de las otras normas citadas, análisis que se realiza paralelamente sobre dos secciones, una plana y otra de canto, de iguales dimensiones y sometidas a la actuación del mismo momento flector. Finalmente se aplican las formulaciones anteriores a la sección de un forjado, estudiando hormigones de distinta resistencia con tres soluciones de armado en cada caso, comparándose los resultados obtenidos, y

  9. A randomised controlled trial of the probiotic Bifidobacterium breve BBG-001 in preterm babies to prevent sepsis, necrotising enterocolitis and death: the Probiotics in Preterm infantS (PiPS) trial.

    Science.gov (United States)

    Costeloe, Kate; Bowler, Ursula; Brocklehurst, Peter; Hardy, Pollyanna; Heal, Paul; Juszczak, Edmund; King, Andy; Panton, Nicola; Stacey, Fiona; Whiley, Angela; Wilks, Mark; Millar, Michael R

    2016-08-01

    Necrotising enterocolitis (NEC) and late-onset sepsis remain important causes of death and morbidity in preterm babies. Probiotic administration might strengthen intestinal barrier function and provide protection; this is supported by published meta-analyses, but there is a lack of large well-designed trials. To test the use of the probiotic Bifidobacterium breve strain BBG-001 to prevent NEC, late-onset sepsis and death in preterm babies while monitoring probiotic colonisation of participants. Double-blind, randomised, placebo-controlled trial. Recruitment was carried out in 24 hospitals, and the randomisation programme used a minimisation algorithm. Parents, clinicians and outcome assessors were blinded to the allocation. Babies born between 23 and 30 weeks' gestation and randomised within 48 hours of birth. Exclusions included life-threatening or any gastrointestinal malformation detected within 48 hours of birth and no realistic chance of survival. Active intervention: 1 ml of B. breve BBG-001 in one-eighth-strength infant formula Neocate(®) (Nutricia Ltd, Trowbridge, UK), (6.7 × 10(7) to 6.7 × 10(9) colony-forming units) per dose administered enterally. Placebo: 1 ml of one-eighth-strength infant formula Neocate. Started as soon as practicable and continued daily until 36 weeks' postmenstrual age. Primary outcomes were an episode of bloodstream infection, with any organism other than a skin commensal, in any baby between 72 hours and 46 weeks' postmenstrual age; an episode of NEC Bell stage ≥ 2 in any baby; and death before discharge from hospital. Secondary outcomes included stool colonisation with B. breve. In total, 654 babies were allocated to receive probiotic and 661 to receive placebo over 37 months from July 2010. Five babies were withdrawn; 650 babies from the probiotic group and 660 from the placebo group were included in the primary analysis. Baseline characteristics were well balanced. There was no evidence of benefit for the primary

  10. breve panorámica

    Directory of Open Access Journals (Sweden)

    Sandra M. Camelo Roa

    2005-01-01

    Full Text Available Desde los terrenos de la psicoinmunología, la presente revisión se propone una mirada panorámica a sus principales postulados, líneas de investigación, resultados y perspectivas. Para ello, inicialmente se abordaron algunos aspectos históricos de la relación entre la vida psíquica y la salud; posteriormente, se revisaron aspectos como la fisiología del sistema inmune especificando los tipos de inmunidad adquirida e inmunidad natural. Temas como las interacciones entre el sistema inmune y cerebro fueron revisados desde la evidencia experimental y resumidos en cuatro grupos: condicionamiento de la respuesta inmune, lesiones cerebrales, estrés e inmunidad y emociones e inmunidad. Finalmente, se contemplaron algunos métodos para el estudio del sistema inmune como los ensayos in vitro, citometría de flujo y ensayos in vivo. Como observaciones finales, toda la literatura sugiere que existe relación entre el sistema inmune y diversos estados psíquicos. Pero aún surgen preguntas de esta indiscutible relación sin tener una respuesta satisfactoria.

  11. Breve Manuale di Geografia Umana

    NARCIS (Netherlands)

    Minca, C.; Colombino, A.

    2012-01-01

    Il percorso per andare al lavoro, la scelta sul dove trascorrere le prossime vacanze, le nostre opinioni sulla politica estera, le nostre preoccupazioni per la crisi economica, le nostre affermazioni di identità, il nostro desiderio di fuga dal quotidiano, perfino la scelta di acquistare una casa in

  12. Transferência e complexo de édipo, na obra de Freud: notas sobre os destinos da transferência Transference and oedipus complex, within Freud's theory: notes about the transference destiny

    Directory of Open Access Journals (Sweden)

    Lara Cristina d'Avila Lourenço

    2005-04-01

    Full Text Available Este artigo comunica algumas considerações sobre o conceito de transferência, na teoria freudiana. Especial atenção é voltada para as relações entre transferência, complexo de Édipo e complexo de castração. Partindo dessas relações e da hipótese segundo a qual Freud não apresenta um final possível para o complexo de Édipo, o presente trabalho entende que esse autor não fornece uma teoria sobre o final da transferência. Esse pensamento evoca o impasse freudiano em relação aos términos das análises. Nesse ponto, é feita uma breve referência à teoria lacaniana sobre a castração e a transferência ao final de análise.This article discloses some considerations about the transference conception situated within Freud's theory. The relations between transference, Oedipus complex and castration complex are highlighted. Starting from these relations and from the allegedly in wich Freud doesn't reveal a possible ending to Oedipus complex, this current work perceives that this author does not provide a theory about the transference ending. This idea drives into Freud's impasse, as far as the analysis ending is concerned. In this issue, a short reference is produced on Lacan's castration theory and on that of the transference at end of the analysis.

  13. II Jornadas sobre Emprendimiento Social y Colectivo

    OpenAIRE

    Centro de Investigación en Gestión de Empresas

    2013-01-01

    La línea temática principal del encuentro es el emprendimiento social y colectivo así como las diferentes líneas de investigación de los institutos y centros de la Red relacionadas, como: responsabilidad social, empleo y reincorporación al trabajo, inserción laboral, servicios sociales y dependencia, género, cambios legislativos, utilidad social, medio ambiente, desarrollo local,... Centro de Investigación en Gestión de Empresas (2013). II Jornadas sobre Emprendimiento Social y Colectivo. ...

  14. Literatura infantil sobre problemas infantiles: la diabetes

    OpenAIRE

    Lázaro Fernández, Patricia

    2015-01-01

    Debido al creciente número de casos de diabetes infantil que se está dando en la sociedad actual, no es raro encontrarse en las aulas cada vez más casos de esta enfermedad. Por esta razón, he decidido analizar dos libros sobre esta temática, para hacer un acercamiento del material seleccionado a las aulas y poder desarrollar junto con los niños un aprendizaje colectivo y cooperativo. En este trabajo se expone la importancia de la literatura y la literatura infantil como una ...

  15. Soportes apoyados sobre vigas pretensadas (EE. UU.

    Directory of Open Access Journals (Sweden)

    Editorial, Equipo

    1965-12-01

    Full Text Available This work has been awarded a prize by the Prestreesed Concrete Institute. It refers to a number of flyovers on the Arroyo Seco, of California. This river has been canalized, to help drainage in case of heavy rainfall. The canal runs through a highly populated zone, and a motor road and accessory ramps had to cross the canal. The resulting design involves three prestressed girders, lightly arched in a parabolic outline, resting on each river shore, running along the line of the bridge, over the canal. It was decided that the bridge piles should not rest on the river bed, in order not to reduce its flow capacity. The girders of a maximum length of 39 ms, are box shaped, and stiffened with thin diaphragms. The cylindrical pile, 1.50 m in diameter, is an integral part of the girder, and applies to it a concentrated load of about 4000 tons. The girders rest on reinforced concrete cubes, which in turn are supported by concrete slabs and piles driven into the soil.Esta obra ha sido premiada por el Prestressed Concrete Institute y se refiere a una serie de pasos superiores sobre el Arroyo Seco, en California, que se ha canalizado para servir de desagüe en el caso de avenidas torrenciales. El canal se halla en una zona de edificación densa y se trata del paso—sobre aquél—de una autopista y rampas de acceso. La solución adoptada consistió en tres vigas pretensadas, ligeramente arqueadas con perfil parabólico, apoyadas en las dos márgenes y situadas en correspondencia con los tres soportes, sobre el propio canal, ya que ninguno de aquéllos debía apoyarse en el lecho de este último al objeto de no disminuir su capacidad de desagüe. Las vigas—de unos 39 m de longitud la mayor—son del tipo cajón, rigidizadas con unos diafragmas de pequeño espesor. El soporte cilíndrico, de 1,50 m de diámetro, se solidariza con la viga y le transmite una notable carga concentrada, del orden de 4.000 toneladas. Los apoyos de estas vigas son simples dados

  16. Ensayo sobre la melancolía

    Directory of Open Access Journals (Sweden)

    Marta Chacón Salinas

    2013-07-01

    Full Text Available Este trabajo es un intento de delimitar la melancolía del conjunto de los trastornos depresivos y de reflexionar sobre sus características tal como aparecen en el discurso de los melancólicos. Se consideran algunas hipótesis en la línea psicoanalítica freudiana y lacaniana (Lambottc, Kristeva para entender cómo se va constituyendo en sus características principales: la intolerancia a la pérdida del objeto y el desfallecimiento del símbolo que le impide elaborar este duelo.

  17. Comentarios sobre dibujo analítico

    OpenAIRE

    Mata Botella, Elena

    2004-01-01

    Cuaderno de apoyo a la docencia del DIBUJO DE ARQUITECTURA que pretende hacer reflexionar al alumno (de primero o segundo de carrera) sobre un tipo de dibujo que aquí se ha llamado “dibujo analítico”. Un dibujo que a través de operaciones gráficas y conceptuales como la esquematización y la selección de información, tiende a alejarse de la descripción del objeto arquitectónico para adentrarse en determinados aspectos o dimensiones que subyacen en el proyecto arquitectónico. Unas notas intr...

  18. Sobre la evaluación docente

    OpenAIRE

    Chamizo, José Antonio

    2012-01-01

    Las universidades requieren una reflexión profunda sobre sus metas primordiales para construir una cultura de la evaluación de la actividad de enseñanza, la cual es diferente de la de investigación. La idea principal es que el proceso de evaluación sumativa, burocrático, superficial y cerrado, sea transformado en uno que involucre a los profesores y sea y abierto. Universities require a deep reflection on their ultimate goals to build a culture of evaluation on teaching activities, which a...

  19. Refuerzo de pavimentos sobre arcillas expansivas

    OpenAIRE

    Zornberg, Jorge

    2012-01-01

    El refuerzo de la capa base de pavimentos se ha utilizado a los efectos de: (i) aumentar la vida útil de un pavimento manteniendo el espesor de la capa base, o (ii) disminuir el espesor de la capa base manteniendo la vida útil del pavimento. Este artículo describe una tercera aplicación del refuerzo basal de pavimentos, a saber, la mitigación de grietas longitudinales inducidas en pavimentos construidos sobre subrasantes compuestas de arcillas expansivas. Aunque todavía existe la necesidad de...

  20. Cinco puntos sobre la revista Barcelona

    OpenAIRE

    Bertone Crippa, Mauro

    2012-01-01

    El humor, como género y narrativa mediática, ocupa un lugar destacado al interior del campo del periodismo gráfico argentino. Como emergente contemporáneo dentro de este ámbito, la revista Barcelona se vuelve un objeto de estudio sugestivo al trabajar, a partir del registro del humor, sobre la agenda y la enunciación de los medios gráficos de actualidad. De esta manera, se analizan las condiciones de producción del discurso de Barcelona para ver qué novedades aporta al campo del hu...