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Sample records for breeze training transcript

  1. Warm Breeze

    Institute of Scientific and Technical Information of China (English)

    1999-01-01

    Middle-aged female painter Wang Yingchun is a first-grade artist at the Research Instituteof Chinese Painting. With a solid foundation in: Chinese painting, oil painting andsculpture she began to experiment in the early 1980s with stone carving, murals, folkart, landscapes, flowers and birds, cubism, expressionism and abstractionism. Living ina time of social transformation, she felt pressed to create her own artistic style. Aftervisiting South America, she produced a batch of works which drew the essence of theBeast Group and used a new technique, without sketching the contours of flowers, sothat the paintings look wild, romantic and exuberant. This painting Warm Breeze displaysWang’s style: While extensively studying the paintings of various schools, she makes hertraditional Chinese ink paintings tinted with modern color.

  2. Using Virtual Reference Transcripts for Staff Training.

    Science.gov (United States)

    Ward, David

    2003-01-01

    Describes a method of library staff training based on chat transcript analysis in which graduate student workers at a university reference desk examined transcripts of actual virtual reference desk transactions to analyze reference interviews. Discusses reference interview standards, reference desk behavior, and reference interview skills in…

  3. A speech-perception training tool to improve phonetic transcription

    Science.gov (United States)

    Padgitt, Noelle R.; Munson, Benjamin; Carney, Edward J.

    2005-09-01

    University instruction in phonetics requires students to associate a set of quasialphabetic symbols and diacritics with speech sounds. In the case of narrow phonetic transcription, students are required to associate symbols with sounds that do not function contrastively in the language. This learning task is challenging, given that students must discriminate among different variants of sounds that are not used to convey differences in lexical meaning. Consequently, many students fail to learn phonetic transcription to a level of proficiency needed for practical application (B. Munson and K. N. Brinkman, Am. J. Speech Lang. Path. [2004]). In an effort to improve students' phonetic transcription skills, a computerized training program was developed to trains students' discrimination and identification of selected phonetic contrasts. The design of the training tool was based on similar tools that have been used to train phonetic contrasts in second-language learners of English (e.g., A. Bradlow et al., J. Acoust. Soc. Am. 102, 3115 [1997]). It consists of multiple stages (bombardment, discrimination, identification) containing phonetic contrasts that students have identified as particularly difficult to perceive. This presentation will provide a demonstration of the training tool, and will present preliminary data on the efficacy of this tool in improving students' phonetic transcription abilities.

  4. The Impact of Endurance Training on Human Skeletal Muscle Memory, Global Isoform Expression and Novel Transcripts.

    Directory of Open Access Journals (Sweden)

    Maléne E Lindholm

    2016-09-01

    Full Text Available Regularly performed endurance training has many beneficial effects on health and skeletal muscle function, and can be used to prevent and treat common diseases e.g. cardiovascular disease, type II diabetes and obesity. The molecular adaptation mechanisms regulating these effects are incompletely understood. To date, global transcriptome changes in skeletal muscles have been studied at the gene level only. Therefore, global isoform expression changes following exercise training in humans are unknown. Also, the effects of repeated interventions on transcriptional memory or training response have not been studied before. In this study, 23 individuals trained one leg for three months. Nine months later, 12 of the same subjects trained both legs in a second training period. Skeletal muscle biopsies were obtained from both legs before and after both training periods. RNA sequencing analysis of all 119 skeletal muscle biopsies showed that training altered the expression of 3,404 gene isoforms, mainly associated with oxidative ATP production. Fifty-four genes had isoforms that changed in opposite directions. Training altered expression of 34 novel transcripts, all with protein-coding potential. After nine months of detraining, no training-induced transcriptome differences were detected between the previously trained and untrained legs. Although there were several differences in the physiological and transcriptional responses to repeated training, no coherent evidence of an endurance training induced transcriptional skeletal muscle memory was found. This human lifestyle intervention induced differential expression of thousands of isoforms and several transcripts from unannotated regions of the genome. It is likely that the observed isoform expression changes reflect adaptational mechanisms and processes that provide the functional and health benefits of regular physical activity.

  5. Cosmic rays in a galactic breeze

    Science.gov (United States)

    Taylor, Andrew M.; Giacinti, Gwenael

    2017-01-01

    Motivated by the discovery of the nonthermal Fermi bubble features both below and above the Galactic plane, we investigate a scenario in which these bubbles are formed through galacto-centric outflow. Cosmic rays (CR) both diffusing and advecting within a galactic breeze outflow, interacting with the ambient gas present, give rise to γ -ray emission, providing an approximately flat surface brightness profile of this emission, as observed. Applying the same outflow profile further out within the disk, the resultant effects on the observable CR spectral properties are determined. A hardening in the spectra due to the competition of advective and diffusive propagation within a particular energy range is noted, even in the limiting case of equal CR diffusion coefficients in the disk and halo. It is postulated that this hardening effect may relate to the observed hardening feature in the CR spectrum at a rigidity of ≈200 GV .

  6. Cosmic Rays in a Galactic Breeze

    CERN Document Server

    Taylor, Andrew M

    2016-01-01

    Motivated by the discovery of the non-thermal Fermi bubble features both below and above the Galactic plane, we investigate a scenario in which these bubbles are formed through Galacto-centric outflow. Cosmic rays (CR) both diffusing and advecting within a Galactic breeze outflow, interacting with the ambient gas present, give rise to gamma-ray emission, providing an approximately flat surface brightness profile of this emission, as observed. Applying the same outflow profile further out within the disk, the resultant effects on the observable CR spectral properties are determined. A hardening in the spectra due to the competition of advective and diffusive propagation within a particular energy range is noted, even in the limiting case of equal CR diffusion coefficients in the disk and halo. It is postulated that this hardening effect may relate to the observed hardening feature in the CR spectrum at a rigidity of $\\approx 200$ GV.

  7. The Influence of Soil Moisture, Coastline Curvature, and Land-Breeze Circulations on Sea-Breeze Initiated Precipitation

    Science.gov (United States)

    Baker, David R.; Lynn, Barry H.; Boone, Aaron; Tao, Wei-Kuo; Simpson, Joanne

    2000-01-01

    Idealized numerical simulations are performed with a coupled atmosphere/land-surface model to identify the roles of initial soil moisture, coastline curvature, and land breeze circulations on sea breeze initiated precipitation. Data collected on 27 July 1991 during the Convection and Precipitation Electrification Experiment (CAPE) in central Florida are used. The 3D Goddard Cumulus Ensemble (GCE) cloud resolving model is coupled with the Goddard Parameterization for Land-Atmosphere-Cloud Exchange (PLACE) land surface model, thus providing a tool to simulate more realistically land-surface/atmosphere interaction and convective initiation. Eight simulations are conducted with either straight or curved coast-lines, initially homogeneous soil moisture or initially variable soil moisture, and initially homogeneous horizontal winds or initially variable horizontal winds (land breezes). All model simulations capture the diurnal evolution and general distribution of sea-breeze initiated precipitation over central Florida. The distribution of initial soil moisture influences the timing, intensity and location of subsequent precipitation. Soil moisture acts as a moisture source for the atmosphere, increases the connectively available potential energy, and thus preferentially focuses heavy precipitation over existing wet soil. Strong soil moisture-induced mesoscale circulations are not evident in these simulations. Coastline curvature has a major impact on the timing and location of precipitation. Earlier low-level convergence occurs inland of convex coastlines, and subsequent precipitation occurs earlier in simulations with curved coastlines. The presence of initial land breezes alone has little impact on subsequent precipitation. however, simulations with both coastline curvature and initial land breezes produce significantly larger peak rain rates due to nonlinear interactions.

  8. Dynamical analysis of sea-breeze hodograph rotation in Sardinia

    Directory of Open Access Journals (Sweden)

    N. Moisseeva

    2014-09-01

    Full Text Available This study investigates the diurnal evolution of sea-breeze rotation over an island in the mid-latitudes. Earlier research on sea-breezes in Sardinia shows that the onshore winds around various coasts of the island exhibit both the theoretically predicted clockwise rotation as well as seemingly anomalous anti-clockwise rotation. A non-hydrostatic fully compressible numerical model (WRF is used to simulate wind fields on and around the island on previously-studied sea-breeze days and is shown to accurately capture the circulation on all coasts. Diurnal rotation of wind is examined and patterns of clockwise and anti-clockwise rotation are identified. A dynamical analysis is performed by extracting individual forcing terms from the horizontal momentum equations. Analysis of several regions around the island shows that the direction of rotation is a result of a complex interaction between near-surface and synoptic pressure gradient, Coriolis and advection forcings. An idealized simulation is performed over an artificial island with dramatically simplified topography, yet similar dimensions and latitude to Sardinia. Dynamical analysis of the idealized case reveals a rather different pattern of hodograph rotation to the real Sardinia, yet similar underlying dynamics. The research provides new insights into the dynamics underlying sea-breeze hodograph rotation, especially in coastal zones with complex topography and/or coastline.

  9. Cortical gene transcription response patterns to water maze training in aged mice

    Directory of Open Access Journals (Sweden)

    Martin Bronwen

    2011-06-01

    Full Text Available Abstract Background The hippocampus mediates the acquisition of spatial memory, but the memory trace is eventually transferred to the cortex. We have investigated transcriptional activation of pathways related to cognitive function in the cortex of the aged mouse by analyzing gene expression following water maze training. Results We identified genes that were differentially responsive in aged mice with accurate spatial performance during probe trials or repeated swimming sessions, relative to home cage conditions. Effective learners exhibited significantly greater activation of several pathways, such as the mitogen-activated protein kinase and insulin receptor signaling pathways, relative to swimmers. The genes encoding activity-related cytoskeletal protein (Arc and brain-derived neurotrophic factor (BDNF were upregulated in proficient learners, relative to swimmers and home cage controls, while the gene encoding Rho GTPase activating protein 32 (GRIT was downregulated. We explored the regulation of Arc, BDNF, and GRIT expression in greater morphological detail using in situ hybridization. Recall during probe trials enhanced Arc expression across multiple cortical regions involved in the cognitive component of water maze learning, while BDNF expression was more homogeneously upregulated across cortical regions involved in the associational and sensorimotor aspects of water maze training. In contrast, levels of GRIT expression were uniformly reduced across all cortical regions examined. Conclusions These results suggest that cortical gene transcription is responsive to learning in aged mice that exhibit behavioral proficiency, and support a distributed hypothesis of memory storage across multiple cortical compartments.

  10. Granulation of coke breeze fine for using in the sintering process

    Directory of Open Access Journals (Sweden)

    Mohamed F.M.

    2010-01-01

    Full Text Available Coke breeze is the main fuel used in the sintering process. The value of -3+1 mm. represents the most favorable particle size for coke breeze in the sintering process. About 20% of total coke fines (-0.5 mm are produced during different steps of preparation. Introducing these fines during the sintering process proves to be very harmful for different operating parameters. Thus ,this study aims at investigating the production of granules resulting from these fines using molasses as organic binder and its application in sintering of an iron ore. The results showed that the granules having the highest mechanical properties were obtained with 14.5 wt % molasses addition. The sintering experiments were performed by using coke breeze in different shapes (-3+1 mm in size, coke breeze without sieving and coke breeze granules -3+1 mm. The reduction experiments, microscopic structure and X-ray analysis for the produced sinter were carried out. The results revealed that, all sinter properties (such as shatter test, productivity of sinter machine and blast furnace, reduction time and chemical composition for produced sinter by using coke breeze with size -3+1 mm and coke breeze granules were almost the same. The iron ore sinter which was produced by using coke breeze without sieving yielded low productivity for both sinter machine and blast furnace. Furthermore, using coke breeze without sieving in sintering of an iron ore decreases the vertical velocity of sinter machine and increases the reduction time.

  11. Sea Breeze Front Storm and Its Composite Analysis in Beibu Gulf

    Institute of Scientific and Technical Information of China (English)

    2011-01-01

    [Objective] The aim was to study the sea breeze front storm and its composite analysis in Beibu Gulf. [Method] By dint of routine observation data, ground automatic meteorology data and European numerical mode grid data, rainstorm caused by sea breeze in Guangxi during 1990 and 2007 and the strong convection triggered by sea breeze on June 5 in 2008 were expounded. The main characteristics of the configuration of circulation in the low and high latitude of breeze front of middle scale were summarized. [Resu...

  12. Sea breezes and coastal jets in southeastern Spain

    Science.gov (United States)

    Kottmeier, Christoph; Palacio-Sese, Pablo; Kalthoff, Norbert; Corsmeier, Ulrich; Fiedler, Franz

    2000-11-01

    Sea breezes and low-level jet (LLJ) streams are studied in the region between the Eastern Castilla-La Mancha and the Mediterranean coast of southeastern Spain. The simplified concept of two-dimensional terrain and meteorological conditions explains the spatially and diurnally varying wind system consistently. The changes, as a function of time, height and distance to the coast, of temperature, pressure and wind as well as of certain observed phenomena, such as LLJs and strongly baroclinic zones, result from the response of the mesoscale flow to differential heating. Wind changes are generally found to be consistent with mesoscale changes in geostrophic winds as estimated from station pressure measurements. The LLJs mark the penetration depth of the sea breeze during daytime, where a baroclinic zone develops at the transition between continental air masses and marine air masses, typically at a distance of 150 km from the coast. The analysis is based on aerological data and ground-based measurements from the European Field Experiment in a Desertification Threatened Area (EFEDA) experiments in 1991 and 1994 as well as on 3-hourly data from three synoptic stations of the Spanish meteorological network for April-October in 1991 and 1994. After corrections were made for instrument errors and atmospheric tides, diurnal pressure variations document vertically integrated mass fluxes perpendicular to the coast. Amplitudes of diurnal pressure and temperature changes scatter significantly and are largest in midsummer. They are weakly correlated and pressure amplitudes remain large in spring and autumn when diurnal temperature changes are reduced. With about 6 h delay to the regular development of onshore winds at the coast during daytime, pronounced wind peaks are observed over the eastern plateau at Albacete and Barrax in the late afternoon. Case studies show that they are accompanied by development of baroclinically driven LLJs, whereas winds at Tomelloso, 220-km inland

  13. Accuracy of Wind Prediction Methods in the California Sea Breeze

    Science.gov (United States)

    Sumers, B. D.; Dvorak, M. J.; Ten Hoeve, J. E.; Jacobson, M. Z.

    2010-12-01

    In this study, we investigate the accuracy of measure-correlate-predict (MCP) algorithms and log law/power law scaling using data from two tall towers in coastal environments. We find that MCP algorithms accurately predict sea breeze winds and that log law/power law scaling methods struggle to predict 50-meter wind speeds. MCP methods have received significant attention as the wind industry has grown and the ability to accurately characterize the wind resource has become valuable. These methods are used to produce longer-term wind speed records from short-term measurement campaigns. A correlation is developed between the “target site,” where the developer is interested in building wind turbines, and a “reference site,” where long-term wind data is available. Up to twenty years of prior wind speeds are then are predicted. In this study, two existing MCP methods - linear regression and Mortimer’s method - are applied to predict 50-meter wind speeds at sites in the Salinas Valley and Redwood City, CA. The predictions are then verified with tall tower data. It is found that linear regression is poorly suited to MCP applications as the process produces inaccurate estimates of the cube of the wind speed at 50 meters. Meanwhile, Mortimer’s method, which bins data by direction and speed, is found to accurately predict the cube of the wind speed in both sea breeze and non-sea breeze conditions. We also find that log and power law are unstable predictors of wind speeds. While these methods produced accurate estimates of the average 50-meter wind speed at both sites, they predicted an average cube of the wind speed that was between 1.3 and 1.18 times the observed value. Inspection of time-series error reveals increased error in the mid-afternoon of the summer. This suggests that the cold sea breeze may disrupt the vertical temperature profile, create a stable atmosphere and violate the assumptions that allow log law scaling to work.

  14. Application of fine-grained coke breeze fractions in the process of iron ore sintering

    Directory of Open Access Journals (Sweden)

    M. Niesler

    2014-01-01

    Full Text Available The testing cycle, described in the paper, included fine-grained coke breeze granulation tests and iron concentrate sintering tests with the use of selected granulate samples. The use of granulated coke breeze in the sintering process results in a higher process efficiency, shorter sintering duration and fuel saving.

  15. Impacts of topography and land degradation on the sea breeze over eastern Spain

    NARCIS (Netherlands)

    Miao, J.F.; Kroon, L.J.M.; Vilà-Guerau de Arellano, J.; Holtslag, A.A.M.

    2003-01-01

    A three-dimensional non-hydrostatic atmospheric model RAMS, version3b, is used to examine the impact of complex topography on the sea breeze under heterogeneous and degradation land use characteristics. In the study, it is shown that topography plays an important role in the sea-breeze circulation b

  16. Sea breeze-induced wind sea growth in the central west coast of India.

    Digital Repository Service at National Institute of Oceanography (India)

    Aboobacker, V.M.; Seemanth, M.; Samiksha, S.V.; Sudheesh, K.; Kerkar, J.; Vethamony, P.

    in the northwest direction, and grow progressively while propagating towards the coast. Relationships between wind speed and wind sea height have been derived, and they fairly explain the generation of wind seas by the sea breeze system. Since, the land breeze...

  17. The impact of sea breeze under different synoptic patterns on air pollution within Athens basin.

    Science.gov (United States)

    Mavrakou, Thaleia; Philippopoulos, Kostas; Deligiorgi, Despina

    2012-09-01

    Air quality in densely populated urban coastal areas is directly related to the coupling of the synoptic and the local scale flows. The dispersion conditions within Athens basin, under the influence of different meteorological forcings, lead to distinct spatio-temporal air pollution patterns. The aim of the current observational research is to identify and examine the effect of sea breeze under different atmospheric circulation patterns on air pollution levels for a one-year study period (2007). The study employs surface pressure maps, routine meteorological observations at two coastal sites and nitrogen monoxide (NO), nitrogen dioxide (NO(2)) and ozone (O(3)) concentrations from a network of four air quality stations within the Athens basin. A three-step methodology is applied that incorporates a set of criteria for classifying atmospheric circulation and identifying sea breeze events under each circulation pattern. Two types of sea breeze development are identified (pure sea breeze-PSB and modified sea breeze-MSB) with distinct characteristics. Sea breeze is found to develop more frequently under offshore compared to onshore and parallel to the shoreline background flows. Poor dispersion conditions (high nitrogen oxides-NO(x) and O(3) concentrations) are connected to the pure sea breeze cases and to those cases where sea breeze interacts with a moderate northerly flow during the warm period. The levels of NO(x) and O(3) for the northern Athens basin area are found to be significantly higher during the sea breeze days compared to the Etesian days. Regarding the diurnal variation of ozone for the sea breeze days, peak concentrations and higher intra-daily ranges are observed. Day-to-day pollution accumulation (build-up effect) is measured for O(3) at the northern stations in the Athens basin.

  18. Dispersal of seeds by the tropical sea breeze.

    Science.gov (United States)

    Greene, D F; Quesada, M; Calogeropoulos, C

    2008-01-01

    Given the dependence of most wind-pollinated and wind-dispersed species on low relative humidity (RH) for abscission, and the minimization of RH in the early afternoon, there ought to be a marked directional bias in seed dispersal at sites with a strong local diurnal circulation. We filmed the abscission of seeds of five wind-dispersed tropical species near the coast of Mexico (Jalisco). We found that (1) most abscission occurred during the period from 10:00 to 17:00 hours; (2) there was a strong bias for landward dispersal due to the midday sea breeze; (3) the little nocturnal dispersal that occurred was toward the sea (due to the night land breeze); (4) there was no abscission in the absence of wind (i.e., indoors) except for one species; and (5) holding relative humidity constant, the proportion of seeds diurnally abscising is strongly correlated with horizontal wind speed. We predict that a similar bias (this time for upslope dispersal) for xerochastic dispersal will occur in complex terrain. We conclude that in coastal and mountainous terrain, dispersal models (and inverse modeling efforts) must include a directionality term.

  19. Hot Jupiter Breezes: Time-dependent Outflows from Extrasolar Planets

    CERN Document Server

    Owen, James E

    2015-01-01

    We explore the dynamics of magnetically controlled outflows from Hot Jupiters, where these flows are driven by UV heating from the central star. In these systems, some of the open field lines do not allow the flow to pass smoothly through the sonic point, so that steady-state solutions do not exist in general. This paper focuses on this type of magnetic field configuration, where the resulting flow becomes manifestly time-dependent. We consider the case of both steady heating and time-variable heating, and find the time scales for the corresponding time variations of the outflow. Because the flow cannot pass through the sonic transition, it remains subsonic and leads to so-called breeze solutions. One manifestation of the time variability is that the flow samples a collection of different breeze solutions over time, and the mass outflow rate varies in quasi-periodic fashion. Because the flow is subsonic, information can propagate inward from the outer boundary, which determines, in part, the time scale of the...

  20. Idealized WRF model sensitivity simulations of sea breeze types and their effects on offshore windfields

    Science.gov (United States)

    Steele, C. J.; Dorling, S. R.; von Glasow, R.; Bacon, J.

    2013-01-01

    The behaviour and characteristics of the marine component of sea breeze cells have received little attention relative to their onshore counterparts. Yet there is a growing interest and dependence on the offshore wind climate from, for example, a wind energy perspective. Using idealized model experiments, we investigate the sea breeze circulation at scales which approximate to those of the southern North Sea, a region of major ongoing offshore wind farm development. We also contrast the scales and characteristics of the pure and the little known corkscrew and backdoor sea breeze types, where the type is pre-defined by the orientation of the synoptic scale flow relative to the shoreline. We find, crucially, that pure sea breezes, in contrast to corkscrew and backdoor types, can lead to substantial wind speed reductions offshore and that the addition of a second eastern coastline emphasises this effect through generation of offshore "calm zones". The offshore extent of all sea breeze types is found to be sensitive to both the influence of Coriolis acceleration and to the boundary layer scheme selected. These extents range, for example for a pure sea breeze produced in a 2 m s-1 offshore gradient wind, from 0 km to 21 km between the Mellor-Yamada-Nakanishi-Niino and the Yonsei State University schemes respectively. The corkscrew type restricts the development of a backdoor sea breeze on the opposite coast and is also capable of traversing a 100 km offshore domain even under high along-shore gradient wind speed (>15 m s-1) conditions. Realistic variations in sea surface skin temperature and initializing vertical thermodynamic profile do not significantly alter the resulting circulation, though the strengths of the simulated sea breezes are modulated if the effective land-sea thermal contrast is altered. We highlight how sea breeze impacts on circulation need to be considered in order to improve the accuracy of both assessments of the offshore wind energy climate and

  1. Interactions between the thermal internal boundary layer and sea breezes

    Energy Technology Data Exchange (ETDEWEB)

    Steyn, D.G. [The Univ. of British Columbia, Dept. of Geography, Atmospheric Science Programme, Vancouver (Canada)

    1997-10-01

    In the absence of complex terrain, strongly curved coastline or strongly varying mean wind direction, the Thermal Internal Boundary Layer (TIBL) has well known square root behaviour with inland fetch. Existing slab modeling approaches to this phenomenon indicate no inland fetch limit at which this behaviour must cease. It is obvious however that the TIBL cannot continue to grow in depth with increasing fetch, since the typical continental Mixed Layer Depths (MLD) of 1500 to 2000 m must be reached between 100 and 200 km from the shoreline. The anticyclonic conditions with attendant strong convection and light winds which drive the TIBL, also drive daytime Sea Breeze Circulations (SBC) in the coastal zone. The onshore winds driving mesoscale advection of cool air are at the core of TIBL mechanisms, and are invariably part of a SBC. It is to be expected that TIBL and SBC be intimately linked through common mechanisms, as well as external conditions. (au)

  2. The seasonal characteristics of the breeze circulation at a coastal Mediterranean site in South Italy

    DEFF Research Database (Denmark)

    Federico, S.; Pasqualoni, L.; Sempreviva, Anna Maria;

    2010-01-01

    at about 600 m from the coastline in a flat open area at the foot of a mountain chain located in a region of complex orography. We study the seasonal behaviour of the sea-land breeze circulation by analysing two years of hourly data of wind speed and direction, temperature, radiation and relative humidity......We present a study on the characteristics of the sea breeze flow at a coastal site located in the centre of the Mediterranean basin at the southern tip of Italy. This study is finalized to add new data on breeze circulations over a narrow peninsula and present a unique experimental coastal site...... from a surface meteorological station, eighteen-months data from a wind profiler, and two-year data from the ECMWF analysis. Results show that breezes dominate the local circulation and play a major role for the local climate. They are modulated by the season, through the sea-land temperature...

  3. Temperature, comfort and pollution levels during heat waves and the role of sea breeze.

    Science.gov (United States)

    Papanastasiou, Dimitris K; Melas, Dimitris; Bartzanas, Thomas; Kittas, Constantinos

    2010-05-01

    During the summer of 2007 several Greek regions suffered periods of extreme heat, with midday temperatures of over 40 degrees C on several consecutive days. High temperatures were also recorded on the east coast of central Greece, where a complex sea breeze circulation system frequently develops. The more intense events occurred at the end of June and July. The highest temperatures were observed on 26 June and 25 July, while the sea breeze developed only on 25 July. Meteorological data collected at two sites-a coastal urban location and an inland suburban site that is not reached by the sea breeze flow-as well as pollution data collected at the urban site, were analysed in order to investigate the relationship between sea breeze development and the prevailing environmental conditions during these two heat wave events. The analysis revealed that sea breeze development affects temperature and pollution levels at the shoreline significantly, causing a decrease of approximately 4 degrees C from the maximum temperature value and an increase of approximately 30% in peak PM10 levels. Additionally, several stress indices were calculated in order to assess heat comfort conditions at the two sites. It was found that nocturnal comfort levels are determined mainly by the urban heat island effect, the intensity of which reaches up to 8 degrees C, while the applied indices do not demonstrate any significant daytime thermal stress relief due to sea breeze development.

  4. Scaling Characteristics of Developing Sea Breezes Simulated in a Water Tank

    Science.gov (United States)

    Yuan, Renmin; Sun, Jianning; Luo, Tao; Wu, Xuping

    2013-09-01

    Sea-breeze circulations in a stably stratified environment have been simulated in a water tank. The floor of the tank was divided into two halves representing land and sea; the land side was heated from the bottom of the tank, and the sea side was insulated by an underlying sponge slab. The temperature profiles over both land and sea sides, the land-sea temperature difference, and the horizontal temperature distributions were measured. Particle tracking velocimetry was applied to obtain the two-dimensional velocity field orthogonal to the coastline. It was shown that the overall flow consists of a closed circulation caused by the horizontal temperature difference between land and sea, and a strong updraft occurring at the sea-breeze front. The dimensionless governing parameters are calculated from the measurements and used to characterize the developing sea breezes. The analysis confirms the scaling laws for sea-breeze velocity and depth. The results indicate that the scaling characteristics of the sea-breeze translation speed during the developing period are different to those during the following maintaining period. A criterion for the onset of the sea breeze is proposed based on these results.

  5. Idealized WRF model sensitivity simulations of sea breeze types and their effects on offshore windfields

    Directory of Open Access Journals (Sweden)

    C. J. Steele

    2012-06-01

    Full Text Available The behaviour and characteristics of the marine component of sea breeze cells have received little attention relative to their onshore counterparts. Yet there is a growing interest and dependence on the offshore wind climate from, for example, a wind energy perspective. Using idealized model experiments, we investigate the sea breeze circulation at scales which approximate to those of the Southern North Sea, a region of major ongoing offshore wind farm development. We also contrast the scales and characteristics of the pure and the little known corkscrew and backdoor sea breeze types, where the type is pre-defined by the orientation of the synoptic scale flow relative to the shoreline. We find, crucially, that pure sea breezes, in contrast to corkscrew and backdoor types, can lead to substantial wind speed reductions offshore and that the addition of a second eastern coastline emphasises this effect through generation of offshore "calm zones". The offshore extent of all sea breeze types is found to be sensitive to both the influence of Coriolis acceleration and to the boundary layer scheme selected. These extents range, for example for a pure sea breeze produced in a 2 m s−1 offshore gradient wind, from 10 km to 40 km between the Mellor-Yamada-Nakanishi-Niino and the Yonsei State University schemes, respectively. The corkscrew type restricts the development of a backdoor sea breeze on the eastern coast and is also capable of traversing a 100 km offshore domain even under high gradient wind speed (>15 m s−1 conditions. Realistic variations in sea surface skin temperature during the sea breeze season do not significantly affect the circulation, suggesting that a thermal contrast is only needed as a precondition to the development of the sea breeze. We highlight how sea breeze impacts on circulation need to be considered in order to improve the accuracy of

  6. Numerical study of the evolution of a sea-breeze front under two environmental flows

    Science.gov (United States)

    Liang, Zhaoming; Wang, Donghai

    2015-06-01

    The evolution of a sea-breeze front (SBF) in parallel and offshore environmental flows was investigated by using high-resolution simulations of two SBF cases from the Bohai Bay region, China. The results show that the combination of a distinct vertical wind shear caused by the sea-breeze circulation with a neutral or slightly stable atmospheric stratification associated with the thermal inner boundary layer promoted the occurrence and maintenance of a Kelvin-Helmholtz billow (KHB). In a parallel environmental flow, the SBF evolved into a few connected segments because of the inhomogeneity of the sea-breeze direction and intensity as it penetrated inland. A significant upward vertical motion occurred at the two ends of the SBF segment owing to the sea-breeze convergence and was accelerated by the KHB. The KHB made a notable contribution to the intensity at the ends of the segment, whereas the intensity at the middle segment was primarily attributed to the convergence between the sea breeze and the parallel flow. In the offshore environmental flow, the clockwise rotation of the offshore flow varying with time increased the downstream convergence of the interface between the sea breeze and the offshore flow and pushed the downstream convergence zone to an orientation consistent with the offshore flow. The air parcels ascending from the downstream part of the SBF were continuously lifted by the downstream convergence zone during their advection, leading to a significant downstream development of the SBF. The significant upward vertical motion caused by the sea-breeze convergence behind the upstream end of the SBF was shifted to the upstream end of the SBF by the KHB, which enhanced the intensity of the upstream end of the SBF.

  7. Cycle training modulates satellite cell and transcriptional responses to a bout of resistance exercise.

    Science.gov (United States)

    Murach, Kevin A; Walton, R Grace; Fry, Christopher S; Michaelis, Sami L; Groshong, Jason S; Finlin, Brian S; Kern, Philip A; Peterson, Charlotte A

    2016-09-01

    This investigation evaluated whether moderate-intensity cycle ergometer training affects satellite cell and molecular responses to acute maximal concentric/eccentric resistance exercise in middle-aged women. Baseline and 72 h postresistance exercise vastus lateralis biopsies were obtained from seven healthy middle-aged women (56 ± 5 years, BMI 26 ± 1, VO2max 27 ± 4) before and after 12 weeks of cycle training. Myosin heavy chain (MyHC) I- and II-associated satellite cell density and cross-sectional area was determined via immunohistochemistry. Expression of 93 genes representative of the muscle-remodeling environment was also measured via NanoString. Overall fiber size increased ~20% with cycle training (P = 0.052). MyHC I satellite cell density increased 29% in response to acute resistance exercise before endurance training and 50% with endurance training (P training, MyHC I satellite cell density decreased by 13% in response to acute resistance exercise (acute resistance × training interaction, P trained state in response to resistance exercise. Similar satellite cell and gene expression response patterns indicate coordinated regulation of the muscle environment to promote adaptation. Moderate-intensity endurance cycle training modulates the response to acute resistance exercise, potentially conditioning the muscle for more intense concentric/eccentric activity. These results suggest that cycle training is an effective endurance exercise modality for promoting growth in middle-aged women, who are susceptible to muscle mass loss with progressing age.

  8. Idealized WRF model sensitivity simulations of sea breeze types and their effects on offshore windfields

    Directory of Open Access Journals (Sweden)

    C. J. Steele

    2013-01-01

    Full Text Available The behaviour and characteristics of the marine component of sea breeze cells have received little attention relative to their onshore counterparts. Yet there is a growing interest and dependence on the offshore wind climate from, for example, a wind energy perspective. Using idealized model experiments, we investigate the sea breeze circulation at scales which approximate to those of the southern North Sea, a region of major ongoing offshore wind farm development. We also contrast the scales and characteristics of the pure and the little known corkscrew and backdoor sea breeze types, where the type is pre-defined by the orientation of the synoptic scale flow relative to the shoreline. We find, crucially, that pure sea breezes, in contrast to corkscrew and backdoor types, can lead to substantial wind speed reductions offshore and that the addition of a second eastern coastline emphasises this effect through generation of offshore "calm zones". The offshore extent of all sea breeze types is found to be sensitive to both the influence of Coriolis acceleration and to the boundary layer scheme selected. These extents range, for example for a pure sea breeze produced in a 2 m s−1 offshore gradient wind, from 0 km to 21 km between the Mellor-Yamada-Nakanishi-Niino and the Yonsei State University schemes respectively. The corkscrew type restricts the development of a backdoor sea breeze on the opposite coast and is also capable of traversing a 100 km offshore domain even under high along-shore gradient wind speed (>15 m s−1 conditions. Realistic variations in sea surface skin temperature and initializing vertical thermodynamic profile do not significantly alter the resulting circulation, though the strengths of the simulated sea breezes are modulated if the effective land-sea thermal contrast is altered. We highlight how sea breeze impacts on circulation need to be

  9. Breeze transients as triggers for the initiation of deep moist convection on mountains slopes

    Science.gov (United States)

    Giaiotti, D.; Stel, F.; Gladich, I.; Giacomini, A.

    2010-09-01

    Deep moist convection frequency is characterized by seasonal and diurnal cycles, related to the interplay between the different elements which force and sustain it: onset, instability, wind pattern. Besides the instability diurnal cycles, the low level triggers initiating the convective motions are considered responsible for the modulations of the cycle. In some areas, like in the southern side of the Alpine ridge, the onset and the reversal of breezes produce microscale areas of flow convergence, at the lower levels, due breezes transients and breezes interaction with the orography. This work presents a numerical analysis of the convection initiation generated by breezes transients. The Weather Research and Forecasting model (WRF) has been used to make simplified case studies and real cases simulations in which orography, solar radiation exposure and sea surface temperature variations are considered. General results support the thesis that the daily modulation of thunderstorm development is significantly accounted by the trigger effect of breezes transients, when synoptic winds are negligible of course. The results produced by the large set of simulations are compared with the climatological data of convective cells development in the Northeastern Italian region.

  10. Large-eddy simulation of sea breeze at an idealized peninsular site

    Science.gov (United States)

    Rizza, Umberto; Miglietta, Mario Marcello; Anabor, Vagner; Degrazia, Gervasio A.; Maldaner, Silvana

    2015-08-01

    A high-resolution large-eddy simulation (LES) has been performed to simulate a sea-breeze circulation over an idealized peninsular domain. The simulation is forced with the surface latent/sensible heat fluxes and the large-scale horizontal pressure gradient that are obtained from a mesoscale simulation. This methodology allows the investigation of the physical phenomena that are peculiar for a sea-breeze circulation and that generally require spatial resolution approximately equal to 100 m or less. Here, small-scale dynamical effects associated to these phenomena, i.e. the interaction between the sea-breeze front with the convective turbulence generated over-land, the formation of the zero-velocity layer, and the development of the Kelvin-Helmholtz billows, are investigated. Results from the present numerical study have revealed the formation of a zero-velocity layer that is initially near the ground then it rises to define a well-marked sea-breeze depth. Scaling analysis applied to the LES output fields reveals that during the phase of inland penetration the scaling laws for sea-breeze strength and depth have both a proportionality coefficient equal to 0.15.

  11. Lake breezes in the southern Great Lakes region and their influence during BAQS-Met 2007

    Directory of Open Access Journals (Sweden)

    D. M. L. Sills

    2011-02-01

    Full Text Available Mesoscale observations from the BAQS-Met field experiment during the summer of 2007 were integrated and manually analyzed in order to identify and characterize lake breezes in the southern Great Lakes region of North America, and assess their potential impact on air quality. Lake breezes were found to occur on 90% of study days, often occurring in conditions previously thought to impede their development. They affected all parts of the study region, including southwestern Ontario and nearby portions of southeast Michigan and northern Ohio, occasionally penetrating inland from 100 km to over 200 km. Occurrence rates and penetration distances were found to be higher than previously reported in the literature. This more accurate depiction of observed lake breezes allows a better understanding of their influence on the production and transport of pollutants in this region.

    The observational analyses were compared with output from subsequent runs of a high-resolution numerical weather prediction model. The model accurately predicted lake breeze occurrence in a variety of synoptic wind regimes, but selected cases showed substantial differences in the detailed timing and location of lake-breeze fronts, and with the initiation of deep moist convection. Knowledge of such strengths and weaknesses will assist with interpretation of results from air quality modelling driven by this meteorological model.

  12. Warm Breeze from the starboard bow: a new population of neutral helium in the heliosphere

    CERN Document Server

    Kubiak, M A; Sokół, J M; Swaczyna, P; Grzedzielski, S; Alexashov, D B; Izmodenov, V V; Moebius, E; Leonard, T; Fuselier, S A; Wurz, P; McComas, D J

    2014-01-01

    We investigate the signals from neutral He atoms observed from Earth orbit in 2010 by IBEX. The full He signal observed during the 2010 observation season can be explained as a superposition of pristine neutral interstellar He gas and an additional population of neutral He that we call the Warm Breeze. The Warm Breeze is approximately two-fold slower and 2.5 times warmer than the primary interstellar He population, and its density in front of the heliosphere is ~7% that of the neutral interstellar helium. The inflow direction of the Warm Breeze differs by ~19deg from the inflow direction of interstellar gas. The Warm Breeze seems a long-term feature of the heliospheric environment. It has not been detected earlier because it is strongly ionized inside the heliosphere, which brings it below the threshold of detection via pickup ion and heliospheric backscatter glow observations, as well as by the direct sampling of GAS/Ulysses. Possible sources for the Warm Breeze include (1) the secondary population of inters...

  13. Urban effects of Chennai on sea breeze induced convection and precipitation

    Indian Academy of Sciences (India)

    Matthew Simpson; Sethu Raman; R Suresh; U C Mohanty

    2008-12-01

    Doppler radar derived wind speed and direction profiles showed a well developed sea breeze circulation over the Chennai, India region on 28 June, 2003. Rainfall totals in excess of 100 mm resulted from convection along the sea breeze front. Inland propagation of the sea breeze front was observed in radar reflectivity imagery. High-resolution MM5 simulations were used to investigate the influence of Chennai urban land use on sea breeze initiated convection and precipitation. A comparison of observed and simulated 10 m wind speed and direction over Chennai showed that the model was able to simulate the timing and strength of the sea breeze. Urban effects are shown to increase the near surface air temperature over Chennai by 3.0 K during the early morning hours. The larger surface temperature gradient along the coast due to urban effects increased onshore flow by 4.0m s−1. Model sensitivity study revealed that precipitation totals were enhanced by 25 mm over a large region 150 km west of Chennai due to urban effects. Deficiency in model physics related to night-time forecasts are addressed.

  14. The impact of land and sea surface variations on the Delaware sea breeze at local scales

    Science.gov (United States)

    Hughes, Christopher P.

    The summertime climate of coastal Delaware is greatly influenced by the intensity, frequency, and location of the local sea breeze circulation. Sea breeze induced changes in temperature, humidity, wind speed, and precipitation influence many aspects of Delaware's economy by affecting tourism, farming, air pollution density, energy usage, and the strength, and persistence of Delaware's wind resource. The sea breeze front can develop offshore or along the coastline and often creates a near surface thermal gradient in excess of 5°C. The purpose of this dissertation is to investigate the dynamics of the Delaware sea breeze with a focus on the immediate coastline using observed and modeled components, both at high resolutions (~200m). The Weather Research and Forecasting model (version 3.5) was employed over southern Delaware with 5 domains (4 levels of nesting), with resolutions ranging from 18km to 222m, for June 2013 to investigate the sensitivity of the sea breeze to land and sea surface variations. The land surface was modified in the model to improve the resolution, which led to the addition of land surface along the coastline and accounted for recent urban development. Nine-day composites of satellite sea surface temperatures were ingested into the model and an in-house SST forcing dataset was developed to account for spatial SST variation within the inland bays. Simulations, which include the modified land surface, introduce a distinct secondary atmospheric circulation across the coastline of Rehoboth Bay when synoptic offshore wind flow is weak. Model runs using high spatial- and temporal-resolution satellite sea surface temperatures over the ocean indicate that the sea breeze landfall time is sensitive to the SST when the circulation develops offshore. During the summer of 2013 a field campaign was conducted in the coastal locations of Rehoboth Beach, DE and Cape Henlopen, DE. At each location, a series of eleven small, autonomous thermo-sensors (i

  15. Breezes Along the Coast of Tamaulipas, Northeastern Mexico: Contribution to the Wind Energy Potencial

    Science.gov (United States)

    Orozco, J. M.; Rivas, D.

    2012-12-01

    The near-surface wind along the coast of northeastern Mexico and western Gulf of Mexico, with emphasis on the breeze regime, is characterized. Wind data from the North American Regional Reanalysis (NARR) for the period from 2000 to 2010 are used, which are validated by comparison with surface in-situ observations (weather stations). The validation shows a good agreement between the NARR product and the observations. The preliminary results show that the diurnal frequency associated with breezes has a significant contribution to the variability of the wind in the study area. This suggests that the breeze regime can be important for the wind power generation, and given the extensive coastal plain of Tamaulipas, the implementation of electricity-generation prototypes in the area is possible.

  16. 78 FR 62300 - Prairie Breeze Wind Energy LLC; Supplemental Notice That Initial Market-Based Rate Filing...

    Science.gov (United States)

    2013-10-15

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF ENERGY Federal Energy Regulatory Commission Prairie Breeze Wind Energy LLC; Supplemental Notice That Initial Market... in the above-referenced proceeding, of Prairie Breeze Wind Energy LLC's application for...

  17. Land Breeze and Thermals: A Scale Threshold to Distinguish Their Effects

    Institute of Scientific and Technical Information of China (English)

    2005-01-01

    Land breeze is a type of mesoscale circulation developed due to thermal forcing over a heterogeneous landscape. It can contribute to atmospheric dynamic and hydrologic processes through affecting heat and water fluxes on the land-atmosphere interface and generating shallow convective precipitation. If the scale of the landscape heterogeneity is smaller than a certain size, however, the resulting land breeze becomes weak and becomes mixed up with other thermal convections like thermals. This study seeks to identify a scale threshold to distinguish the effects between land breeze and thermals. Two-dimensional simulations were performed with the Regional Atmospheric Modeling System (RAMS) to simulate thermals and land breeze. Their horizontal scale features were analyzed using the wavelet transform. The thermals developed over a homogeneous landscape under dry or wet conditions have an initial scale of 2-5 km during their early stage of development. The scale jumps to 10-15 km when condensation occurs. The solution of an analytical model indicates that the reduced degree of atmospheric instability due to the release of condensation potential heat could be one of the contributing factors for the increase in scale.The land breeze, on the other hand, has a major scale identical to the size of the landscape heterogeneity throughout various stages of development. The results suggest that the effects of land breeze can be clearly distinguished from those of thermals only if the size of the landscape heterogeneity is larger than the scale threshold of about 5 km for dry atmospheric processes or about 15 km for moist ones.

  18. Transforming the Transcript to Reflect the Whole Scholar: Legitimizing Pedagogical Training for Graduate Student Instructors

    Science.gov (United States)

    Iorio, Josh; Decker, Mark Lowry

    2011-01-01

    Although universities acknowledge that teacher training is critical for ensuring quality undergraduate education, research has repeatedly demonstrated that universities typically do an inadequate job of preparing graduate students for their instructor role. In this paper, we show that both graduate students and universities find the pedagogical…

  19. Power training and postmenopausal hormone therapy affect transcriptional control of specific co-regulated gene clusters in skeletal muscle

    Science.gov (United States)

    Fey, Vidal; Törmäkangas, Timo; Ronkainen, Paula H. A.; Taaffe, Dennis R.; Takala, Timo; Koskinen, Satu; Cheng, Sulin; Puolakka, Jukka; Kujala, Urho M.; Suominen, Harri; Sipilä, Sarianna; Kovanen, Vuokko

    2010-01-01

    At the moment, there is no clear molecular explanation for the steeper decline in muscle performance after menopause or the mechanisms of counteractive treatments. The goal of this genome-wide study was to identify the genes and gene clusters through which power training (PT) comprising jumping activities or estrogen containing hormone replacement therapy (HRT) may affect skeletal muscle properties after menopause. We used musculus vastus lateralis samples from early stage postmenopausal (50–57 years old) women participating in a yearlong randomized double-blind placebo-controlled trial with PT and HRT interventions. Using microarray platform with over 24,000 probes, we identified 665 differentially expressed genes. The hierarchical clustering method was used to assort the genes. Additionally, enrichment analysis of gene ontology (GO) terms and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways was carried out to clarify whether assorted gene clusters are enriched with particular functional categories. The analysis revealed transcriptional regulation of 49 GO/KEGG categories. PT upregulated transcription in “response to contraction”—category revealing novel candidate genes for contraction-related regulation of muscle function while HRT upregulated gene expression related to functionality of mitochondria. Moreover, several functional categories tightly related to muscle energy metabolism, development, and function were affected regardless of the treatment. Our results emphasize that during the early stages of the postmenopause, muscle properties are under transcriptional modulation, which both PT and HRT partially counteract leading to preservation of muscle power and potentially reducing the risk for aging-related muscle weakness. More specifically, PT and HRT may function through improving energy metabolism, response to contraction as well as by preserving functionality of the mitochondria. Electronic supplementary material The online version of this

  20. Transforming the Transcript to Reflect the Whole Scholar: Legitimizing Pedagogical Training for Graduate Student Instructors

    Directory of Open Access Journals (Sweden)

    Josh Iorio, PhD

    2011-08-01

    Full Text Available Although universities acknowledge that teacher training is critical for ensuring quality undergraduate education, research has repeatedly demonstrated that universities typically do an inadequate job of preparing graduate students for their instructor role. In this paper, we show that both graduate students and universities find the pedagogical development of graduate students to be a valid endeavor, and while graduate students strive to legitimize their own pedagogical development, universities must more fully and officially engage in the process. We conclude with a short list of recommendations for universities to consider toward the goal of legitimizing graduate student pedagogical development.

  1. Impact of sea breeze on wind-seas off Goa, west coast of India

    Indian Academy of Sciences (India)

    S Neetu; Satish Shetye; P Chandramohan

    2006-04-01

    After withdrawal of the Indian Summer Monsoon and until onset of the next monsoon,i.e.,roughly during November-May, winds in the coastal regions of India are dominated by sea breeze.It has an impact on the daily cycle of the sea state near the coast.The impact is quite significant when large scale winds are weak.During one such event,1 –15 April 1997,a Datawell directional waverider buoy was deployed in 23 m water depth off Goa,west coast of India.Twenty-minute averaged spectra,collected once every three hours,show that the spectrum of sea-breeze-related ‘wind-seas’ peaked at 0.23 ± 0.05 Hz. These wind-seas were well separated from swells of frequencies less than 0.15 Hz.The TMA spectrum (Bouws et al 1985) matched the observed seas spectra very well when the sea-breeze was active and the fetch corresponding to equilibrium spectrum was found to be 77 ± 43 km during such occasions. We emphasize on the diurnal cycle of sea-breeze-related sea off the coast of Goa and write an equation for the energy of the seas as a function of the local wind.

  2. Impact of Bay-Breeze Circulations on Surface Air Quality and Boundary Layer Export

    Science.gov (United States)

    Loughner, Christopher P.; Tzortziou, Maria; Follette-Cook, Melanie; Pickering, Kenneth E.; Goldberg, Daniel; Satam, Chinmay; Weinheimer, Andrew; Crawford, James H.; Knapp, David J.; Montzka, Denise D.; Diskin, Glenn S.; Dickerson, Russell R.

    2014-01-01

    Meteorological and air-quality model simulations are analyzed alongside observations to investigate the role of the Chesapeake Bay breeze on surface air quality, pollutant transport, and boundary layer venting. A case study was conducted to understand why a particular day was the only one during an 11-day ship-based field campaign on which surface ozone was not elevated in concentration over the Chesapeake Bay relative to the closest upwind site and why high ozone concentrations were observed aloft by in situ aircraft observations. Results show that southerly winds during the overnight and early-morning hours prevented the advection of air pollutants from the Washington, D.C., and Baltimore, Maryland, metropolitan areas over the surface waters of the bay. A strong and prolonged bay breeze developed during the late morning and early afternoon along the western coastline of the bay. The strength and duration of the bay breeze allowed pollutants to converge, resulting in high concentrations locally near the bay-breeze front within the Baltimore metropolitan area, where they were then lofted to the top of the planetary boundary layer (PBL). Near the top of the PBL, these pollutants were horizontally advected to a region with lower PBL heights, resulting in pollution transport out of the boundary layer and into the free troposphere. This elevated layer of air pollution aloft was transported downwind into New England by early the following morning where it likely mixed down to the surface, affecting air quality as the boundary layer grew.

  3. The Effects of Highly Detailed Urban Roughness Parameters on a Sea-Breeze Numerical Simulation

    Science.gov (United States)

    Varquez, Alvin Christopher G.; Nakayoshi, Makoto; Kanda, Manabu

    2015-03-01

    We consider the effects of detailed urban roughness parameters on a sea-breeze simulation. An urban roughness database, constructed using a new aerodynamic parametrization derived from large-eddy simulations, was incorporated as a surface boundary condition in the advanced Weather Research and Forecasting model. The zero-plane displacement and aerodynamic roughness length at several densely built-up urban grids were three times larger than conventional values due to the consideration of building-height variability. A comparison between simulations from the modified model and its default version, which uses uniform roughness parameters within a conventional method, was conducted for a 2-month period during summer. Results showed a significant improvement in the simulation of surface wind speed but not with temperature. From the 2-month study period, a day with an evident sea-breeze penetration was selected and simulated at higher temporal resolution. Sea-breeze penetration weakened and was more delayed over urbanized areas. The slow sea-breeze penetration also lessened heat advection downwind allowing stronger turbulent mixing and a deeper boundary layer above urban areas. Horizontal wind-speed reduction due to the increased urban surface drag reached heights of several hundreds of metres due to the strong convection.

  4. Idealised numerical simulations of aerodynamic roughness length effects on sea breeze characteristics

    Energy Technology Data Exchange (ETDEWEB)

    Prtenjak, M.T. [Zagreb Univ. (Croatia). Andrija Mohorovicic Geophysical Inst.

    2002-07-01

    A two-dimensional hydrostatic meso-{gamma}-scale model was used to study possible effects of a step change in roughness on the daytime sea breeze. Idealised numerical simulations with the length of aerodynamical roughness (z{sub 0}) ranging from 0.05 m to 1.0 m and by an increase of 0.05 m in all land grid points were made. Two z{sub 0} effects could be pointed out: an increase in the mixing process and a reduction in the wind speeds in the surface layer. An increase of both heights and magnitudes of the sea breeze circulation, caused by a vertical extension of the turbulent field, followed a faster inland penetration of the sea breeze. The second effect resulting from a successive increase in z{sub 0} values was a retardation of the sea breeze front near the ground, visible in its slope. This effect allowed the already faster inland penetration of the marine air high above the land. (orig.)

  5. Object-location training elicits an overlapping but temporally distinct transcriptional profile from contextual fear conditioning.

    Science.gov (United States)

    Poplawski, Shane G; Schoch, Hannah; Wimmer, Mathieu E; Hawk, Joshua D; Walsh, Jennifer L; Giese, Karl P; Abel, Ted

    2014-12-01

    Hippocampus-dependent learning is known to induce changes in gene expression, but information on gene expression differences between different learning paradigms that require the hippocampus is limited. The bulk of studies investigating RNA expression after learning use the contextual fear conditioning task, which couples a novel environment with a footshock. Although contextual fear conditioning has been useful in discovering gene targets, gene expression after spatial memory tasks has received less attention. In this study, we used the object-location memory task and studied gene expression at two time points after learning in a high-throughput manner using a microfluidic qPCR approach. We found that expression of the classic immediate-early genes changes after object-location training in a fashion similar to that observed after contextual fear conditioning. However, the temporal dynamics of gene expression are different between the two tasks, with object-location memory producing gene expression changes that last at least 2 hours. Our findings indicate that different training paradigms may give rise to distinct temporal dynamics of gene expression after learning.

  6. Characteristics of Sea Breeze Front Development with Various Synoptic Conditions and Its Impact on Lower Troposphere Ozone Formation

    Institute of Scientific and Technical Information of China (English)

    Hyo-Eun JI; Soon-Hwan LEE; Hwa-Woon LEE

    2013-01-01

    To examine the correlation between the sizes of sea breeze fronts and pollutants under the influence of synoptic fields,a numerical simulation was conducted in the southeast coastal area of the Korean Peninsula,where relatively high concentrations of pollutants occur because of the presence of various kinds of industrial developments.Sea breeze and sea breeze front days during the period 2005-09 were identified using wind profiler data and,according to the results,the number of days were 72 and 53,respectively.When synoptic forcing was weak,sea breeze fronts moved fast both in horizontal fields and in terms of wind velocity,while in thc case of strong synoptic forcing,sea breeze fronts remained at the coast or moved slowly due to strong opposing flows.In this case,the sea breeze front development function and horizontal potential temperature difference were larger than with weak synoptic forcing.The ozone concentration that moves together with sea breeze fronts was also formed along the frontal surfaces.Ozone advection and diffusion in the case of strong synoptic forcing was suppressed at the frontal surface and the concentration gradient was large.The vertical distribution of ozone was very low due to the Thermal Internal Boundary Layer (TIBL) being low.

  7. Photovoltaic at Hollywood and Desert Breeze Recreational Centers

    Energy Technology Data Exchange (ETDEWEB)

    Ammerman, Shane [Clark County Comprehensive Planning Department, NV (United States)

    2015-09-24

    Executive Summary Renewable Energy Initiatives for Clark County Parks and Recreation Solar Project DOE grant # DE-EE0003180 In accordance with the goals of the Department of Energy (DOE) Office of Energy Efficiency and Renewable Energy for promoting solar energy as clean, carbon-free and cost-effective, the County believed that a recreational center was an ideal place to promote solar energy technologies to the public. This project included the construction of solar electricity generation facilities (40kW) at two Clark County facility sites, Desert Breeze Recreational Center and Hollywood Recreational Center, with educational kiosks and Green Boxes for classroom instruction. The major objectives and goals of this Solar Project include demonstration of state of the art technologies for the generation of electricity from solar technology and the creation of an informative and educational tool in regards to the benefits and process of generating alternative energy. Clark County partnered with Anne Johnson (design architect/consultant), Affiliated Engineers Inc. (AEI), Desert Research Institute (DRI), and Morse Electric. The latest photovoltaic technologies were used in the project to help create the greatest expected energy savings for60443 each recreational center. This coupled with the data created from the monitoring system will help Clark County and NREL further understand the real time outputs from the system. The educational portion created with AEI and DRI incorporates material for all ages with a focus on K - 12. The AEI component is an animated story telling the fundamentals of how sunlight is turned into electricity and DRI‘s creation of Solar Green Boxes brings environmental education into the classroom. In addition to the educational component for the public, the energy that is created through the photovoltaic system also translates into saved money and health benefits for the general public. This project has helped Clark County to further add to its own

  8. Nearshore Coastal Dynamics on a Sea-Breeze Dominated Micro-Tidal Beach (NCSAL)

    Science.gov (United States)

    Torres-Freyermuth, A.; Puleo, J. A.; Ruiz de Alegría-Arzaburu, A.; Figlus, J.; Mendoza, T.; Pintado-Patino, J. C.; Pieterse, A.; Chardon-Maldonado, P.; DiCosmo, N. R.; Wellman, N.; Garcia-Nava, H.; Palemón-Arcos, L.; Roberts, T.; López-González, J.; Bravo, M.; Ojeda, E.; Medellín, G.; Appendini, C. M.; Figueroa, B.; González-Leija, M.; Enriquez, C.; Pedrozo-Acuña, A.; Salles, P.

    2014-12-01

    A comprehensive field experiment devoted to the study of coastal processes on a micro-tidal beach was conducted from March 30th to April 12th 2014 in Sisal, Yucatán México. Wave conditions in the study area are controlled by local (i.e., sea-breezes) and meso-scale (i.e., Nortes) meteorological events. Simultaneous measurements of waves, tides, winds, currents, sediment transport, runup, and beach morphology were obtained in this experiment. Very dense nearshore instrumentation arrays allow us the study of the cross-/along- shore variability of surf/swash zone dynamics during different forcing conditions. Strong sea-breeze wind events produced a diurnal cycle with a maximum wind speed of 14 m/s. The persistent sea-breeze system forces small-amplitude (Hs1 m) Norte event, lasting 48 hours, reached the coast on April 8th generating a long-period swell (Tp>10 s) arriving from the NNW. This event induced an eastward net sediment transport across a wide surf zone. However, long-term observations of sand impoundment at a groin located near the study area suggests that the net sediment transport in the northern Yucatan peninsula is controlled by sea-breeze events and hence swash zone dynamics play an important role in the net sediment budget of this region. A comparative study of surf and swash zone dynamics during both sea-breeze and Norte events will be presented. The Institute of Engineering of UNAM, throughout an International Collaborative Project with the University of Delaware, and CONACYT (CB-167692) provided financial support. The first author acknowledges ONR Global for providing financial support throughout the Visiting Scientist Program.

  9. Photovoltaic at Hollywood and Desert Breeze Recreational Centers

    Energy Technology Data Exchange (ETDEWEB)

    Ammerman, Shane [Clark County Comprehensive Planning Department, NV (United States)

    2015-09-24

    Executive Summary Renewable Energy Initiatives for Clark County Parks and Recreation Solar Project DOE grant # DE-EE0003180 In accordance with the goals of the Department of Energy (DOE) Office of Energy Efficiency and Renewable Energy for promoting solar energy as clean, carbon-free and cost-effective, the County believed that a recreational center was an ideal place to promote solar energy technologies to the public. This project included the construction of solar electricity generation facilities (40kW) at two Clark County facility sites, Desert Breeze Recreational Center and Hollywood Recreational Center, with educational kiosks and Green Boxes for classroom instruction. The major objectives and goals of this Solar Project include demonstration of state of the art technologies for the generation of electricity from solar technology and the creation of an informative and educational tool in regards to the benefits and process of generating alternative energy. Clark County partnered with Anne Johnson (design architect/consultant), Affiliated Engineers Inc. (AEI), Desert Research Institute (DRI), and Morse Electric. The latest photovoltaic technologies were used in the project to help create the greatest expected energy savings for60443 each recreational center. This coupled with the data created from the monitoring system will help Clark County and NREL further understand the real time outputs from the system. The educational portion created with AEI and DRI incorporates material for all ages with a focus on K - 12. The AEI component is an animated story telling the fundamentals of how sunlight is turned into electricity and DRI‘s creation of Solar Green Boxes brings environmental education into the classroom. In addition to the educational component for the public, the energy that is created through the photovoltaic system also translates into saved money and health benefits for the general public. This project has helped Clark County to further add to its own

  10. A case study of sea breeze blocking regulated by sea surface temperature along the English south coast

    Directory of Open Access Journals (Sweden)

    J. K. Sweeney

    2013-09-01

    Full Text Available The sensitivity of sea breeze structure to sea surface temperature (SST and coastal orography is investigated in convection-permitting Met Office Unified Model simulations of a case study along the south coast of England. Changes in SST of 1 K are shown to significantly modify the structure of the sea breeze. On the day of the case study the sea breeze was partially blocked by coastal orography, particularly within Lyme Bay. The extent to which the flow is blocked depends strongly on the static stability of the marine boundary layer. In experiments with colder SST, the marine boundary layer is more stable, and the degree of blocking is more pronounced. The implications of prescribing fixed SST from climatology in numerical weather prediction model forecasts of the sea breeze are discussed.

  11. Impaired transcriptional activity of Nrf2 in age-related myocardial oxidative stress is reversible by moderate exercise training.

    Directory of Open Access Journals (Sweden)

    Sellamuthu S Gounder

    Full Text Available Aging promotes accumulation of reactive oxygen/nitrogen species (ROS/RNS in cardiomyocytes, which leads to contractile dysfunction and cardiac abnormalities. These changes may contribute to increased cardiovascular disease in the elderly. Inducible antioxidant pathways are regulated by nuclear erythroid 2 p45-related factor 2 (Nrf2 through antioxidant response cis-elements (AREs and are impaired in the aging heart. Whereas acute exercise stress (AES activates Nrf2 signaling and promotes myocardial antioxidant function in young mice (~2 months, aging mouse (>23 months hearts exhibit significant oxidative stress as compared to those of the young. The purpose of this study was to investigate age-dependent regulation of Nrf2-antioxidant mechanisms and redox homeostasis in mouse hearts and the impact of exercise. Old mice were highly susceptible to oxidative stress following high endurance exercise stress (EES, but demonstrated increased adaptive redox homeostasis after moderate exercise training (MET; 10m/min, for 45 min/day for ~6 weeks. Following EES, transcription and protein levels for most of the ARE-antioxidants were increased in young mice but their induction was blunted in aging mice. In contrast, 6-weeks of chronic MET promoted nuclear levels of Nrf2 along with its target antioxidants in the aging heart to near normal levels as seen in young mice. These observations suggest that enhancing Nrf2 function and endogenous cytoprotective mechanisms by MET, may combat age-induced ROS/RNS and protect the myocardium from oxidative stress diseases.

  12. NUMERICAL EXPERIMENT ON THE EFFECT OF URBANIZATION UPON SUMMER LAND-SEA BREEZES IN THE COASTLAND OF GUANGXI

    Institute of Scientific and Technical Information of China (English)

    WEN Wei-jun; SHEN Tong-li; DING Zhi-ying; SONG Jie-hui

    2010-01-01

    The 2003-2006 observations were utilized to analyze the surface characteristics of summer land-sea breezes along the coastland of Guangxi and the Weather Research and Forecast model was applied to simulate the breeze structure on August 1-2,2006.Results show that 1)the intensity and distributions of the breezes reproduced from improved urban underlying surface were close to observations.In the daytime the coastwise urban band was a convergent belt of sea breeze,corresponding to the centers of torrential rains; in the nighttime hours the surface of the Gulf of Tonkin(the Vietnamese name)or the Northern Bay(the Chinese name)acted as a convergent zone of land breezes,likely to produce convectivecloud cluster; 2)the experiment on urbanization showed the heat island effect enhancing(weakening)the sea(land)breeze development.Furthermore,the heat island effect mitigated the atmospheric cooling viaradiation over the cities in the night,weakening sinking motion correspondingly,thereby suppressing the dominant factor responsible for the steady development of temperature inversion.As a result,the inversion vigor was reduced greatly,but nevertheless no strong effect of the decreased subsidence was found upon the inversion height.

  13. A climatological study of sea breeze clouds in the southeast of the Iberian Peninsula (Alicante, Spain)

    Energy Technology Data Exchange (ETDEWEB)

    Azorin-Molina, C. [Grupo de Climatologia, Universidad de Barcelona, Barcelona, Cataluna (Spain)]. E-mail: cazorin@ceam.es; Sanchez-Lorenzo, A. [Grupo de Climatologia, Universidad de Barcelona, Barcelona, Cataluna (Spain); Calbo, J. [Grupo de Fisica Ambiental, Universidad de Girona, Campus Montilivi, Cataluna (Spain)

    2009-01-15

    Sea breezes blow under anticyclonic weather types, weak surface pressure gradients, intense solar radiation and relatively cloud-free skies. Generally, total cloud cover must be less than 4/8 in order to cause a thermal and pressure difference between land and sea air which allows the development of this local wind circulation. However, many numerical and observational studies have analyzed the ability of sea breezes to generate clouds in the convective internal boundary layer and in the sea breeze convergence zone. Accordingly, the aim of this study is to statistically analyze the impact of sea breezes on cloud types in the convective internal boundary layer and in the sea breeze convergence zone. The study area is located in the southeast of the Iberian Peninsula (province of Alicante, Spain) and the survey corresponds to a 6-yr study period (2000-2005). This climatological study is mainly based on surface cloud observations at the Alicante-Ciudad Jardin station (central coastal plain) and on an extensive cloud observation field campaign at the Villena-Ciudad station (Prebetic mountain ranges) over a 3-yr study period (2003-2005). The results confirm the hypothesis that the effect of sea breezes on cloud genera is to increase the frequency of low (Stratus) and convective (Cumulus) clouds. Sea breezes trigger the formation of thunderstorm clouds (Cumulonimbus) at the sea breeze convergence zone, which also have a secondary impact on high-level (Cirrus, Cirrocumulus, Cirrostratus), medium-level (Altostratus, Altocumulus) and low-level clouds (Stratus, Stratocumulus, Nimbostratus) associated with the Cumulonimbus clouds (e.g., Cumulonimbus anvil). [Spanish] Las brisas marinas soplan bajo tipos de tiempo anticiclonicos, debiles gradientes de presion atmosferica, radiacion solar intensa y cielos practicamente despejados. Por lo general, la cobertura nubosa total debe ser inferior a 4/8 para que se genere un diferencial termico y de presion entre el aire sobre las

  14. Reducibility mill scale industrial waste via coke breeze at 850-950ºC

    Directory of Open Access Journals (Sweden)

    Gaballah N.M.

    2015-01-01

    Full Text Available Mill scale is a very attractive industrial waste due to its elevated iron content (about = 69.33% Fe besides being suiTab. for direct recycling to the blast furnace via sintering plant. In this paper the characteristics of raw materials and the briquettes produced from this mill scale were studied by different methods of analyses. The produced briquettes were reduced with different amounts of coke breeze at varying temperatures, and the reduction kinetics was determined. The activation energy of this reaction ≈ 61.5 kJ/mole for reduction of mill scale with coke breeze in the form of briquettes with 2% molasses where the chemical reaction interface model is applicable.

  15. The Relationship Between Sea Breeze Forcing and HF Radar-Derived Surface Currents in Monterey Bay

    Science.gov (United States)

    2014-06-01

    and Mr. Richard Lind, to whom I owe great appreciation. I would like to thank Mr. Mike Cook again for making me an expert in Matlab . He also assisted...such as aircraft, lidar , radiosondes and wind profilers, have been used over the years to measure the horizontal and vertical movement of sea breeze...useful, and it was made easy due to the existing MATLAB HFR_Progs toolbox. Using harmonic analysis for winds was not a common idea, but it provided

  16. Investigation of sea-breeze convergence in Salento Peninsula (southeastern Italy)

    Science.gov (United States)

    Comin, Alcimoni Nelci; Miglietta, Mario Marcello; Rizza, Umberto; Acevedo, Otavio Costa; Degrazia, Gervasio Annes

    2015-06-01

    The frequency, the location and the characteristics of convective rainfall events induced by the convergence of different sea breeze systems on a Mediterranean peninsula (Salento, in southeastern Italy) are analyzed. Such events have been studied considering satellite/radar images and output fields from two Limited Area Models in the summer period of 2011-2013. A total of 20 days have been detected in which the precipitation due to sea-breeze convergence was clearly observed in satellite and radar images. The synoptic conditions associated with these events have been identified considering the averages of some relevant meteorological parameters in the selected days and the anomaly with respect to the climate. The presence of a cold trough in the central Mediterranean basin appears as a fundamental ingredient for the occurrence of sea breeze convergence and associated precipitation. High-resolution simulations with two state-of-art numerical models have revealed that both of them are generally able to simulate a convergence pattern correctly, apart from a couple of cases for each model. The higher rainfall amounts occur with weak synoptic wind, and weak-to-moderate values of Convective Available Potential Energy (CAPE). When the synoptic wind is of moderate intensity, the region of convergence moves toward the Adriatic coast for a prevailing southerly component, and toward the Ionian coast for a prevailing northerly component. On the opposite, the skin sea surface temperature is relatively uniform and the difference between the Ionian and the Adriatic Seas, surrounding the peninsula on the east and west side, is generally smaller than 1 K, having only a marginal effect on the sea breeze patterns. Similarly, the value of CAPE before the occurrence of rainfall has low prognostic value. The results shows that limited area models with a grid spacing of few km appear as appropriate tools for the simulation for such relatively small scale phenomena.

  17. A sea breeze induced thunderstorm over an inland station over Indian South Peninsula - A case study

    Science.gov (United States)

    Bhate, Jyoti; Kesarkar, Amit P.; Karipot, Anandakumar; Bala Subrahamanyam, D.; Rajasekhar, M.; Sathiyamoorthy, V.; Kishtawal, C. M.

    2016-10-01

    The dynamic interaction of sea breeze with the prevailing synoptic flows can give rise to meteorological conditions conducive for the occurrence to the thunderstorms over coastal and adjoining regions. Here, we present a rare case study of the genesis of the thunderstorm that occurred on 4th May 2011 at 1500Z over Gadanki (13.5°N, 79.2°E), one of the tropical inland stations (100 km) near to the east coast of the Indian peninsula. The objective of present work is to understand the underlying physical mechanism of initiation of such convection over this region. A set of meteorological observations obtained from microwave radiometer profiler, eddy covariance flux tower system, and Doppler weather radar, are used for investigating the convection genesis characteristics. In conjunction with observations, to bridge the gap of lack of high resolutional spatial observations, the high-resolution (2 km) model analysis is developed using Weather Research and Forecasting (WRF) model and four-dimensional data assimilation technique. The analysis of thermodynamical and dynamical indices carried out from the model analysis as well as observations. Results obtained from this study indicated the presence of a wind discontinuity line and a warm air advection from the north Indian region towards Gadanki caused this area hot dry and convectively active. The sea breeze front propagated over hot and dry area few hours before the genesis of the thunderstorm. The moisture flux convergence increased with the inland propagation of sea breeze front. We found that the inland penetration of sea-breeze front caused advection of moist and cold air over warm and dry region; reduction in dew point depression causing bulging of dry line and lowering of lifting condensation level; development of shear in wind direction and speed; increase in low level convergence and vertical velocity, upward transport of moist air and finally increase in helicity of the environment. The wind shear instability

  18. TRAINS: Dialogue Transcription Tools.

    Science.gov (United States)

    1994-08-01

    speak next? Sacks , Schegloff and Jefferson (1974) propose that conversants use a locally-managed turn-taking protocol. The basic unit is the turn...of Rochester, September. Sacks , Harvey, Emanuel A. Schegloff , and Gail Jefferson . 1974. A simplest systematics for the organization of turn-taking for

  19. Time course analysis reveals gene-specific transcript and protein kinetics of adaptation to short-term aerobic exercise training in human skeletal muscle.

    Science.gov (United States)

    Egan, Brendan; O'Connor, Paul L; Zierath, Juleen R; O'Gorman, Donal J

    2013-01-01

    Repeated bouts of episodic myofibrillar contraction associated with exercise training are potent stimuli for physiological adaptation. However, the time course of adaptation and the continuity between alterations in mRNA expression and protein content are not well described in human skeletal muscle. Eight healthy, sedentary males cycled for 60 min at 80% of peak oxygen consumption (VO2peak) each day for fourteen consecutive days, resulting in an increase in VO2peak of 17.5±3.8%. Skeletal muscle biopsies were taken at baseline, and on the morning following (+16 h after exercise) the first, third, seventh, tenth and fourteenth training sessions. Markers of mitochondrial adaptation (Cyt c and COXIV expression, and citrate synthase activity) were increased within the first week of training, but the mtDNA/nDNA ratio was unchanged by two weeks of training. Accumulation of PGC-1α and ERRα protein during training suggests a regulatory role for these factors in adaptations of mitochondrial and metabolic gene expression. A subset of genes were transiently increased after one training session, but returned to baseline levels thereafter, which is supportive of the concept of transcriptional capacity being particularly sensitive to the onset of a new level of contractile activity. Thus, gene-specific temporal patterns of induction of mRNA expression and protein content are described. Our results illustrate the phenomenology of skeletal muscle plasticity and support the notion that transcript level adjustments, coupled to accumulation of encoded protein, underlie the modulation of skeletal muscle metabolism and phenotype by regular exercise.

  20. Time course analysis reveals gene-specific transcript and protein kinetics of adaptation to short-term aerobic exercise training in human skeletal muscle.

    Directory of Open Access Journals (Sweden)

    Brendan Egan

    Full Text Available Repeated bouts of episodic myofibrillar contraction associated with exercise training are potent stimuli for physiological adaptation. However, the time course of adaptation and the continuity between alterations in mRNA expression and protein content are not well described in human skeletal muscle. Eight healthy, sedentary males cycled for 60 min at 80% of peak oxygen consumption (VO2peak each day for fourteen consecutive days, resulting in an increase in VO2peak of 17.5±3.8%. Skeletal muscle biopsies were taken at baseline, and on the morning following (+16 h after exercise the first, third, seventh, tenth and fourteenth training sessions. Markers of mitochondrial adaptation (Cyt c and COXIV expression, and citrate synthase activity were increased within the first week of training, but the mtDNA/nDNA ratio was unchanged by two weeks of training. Accumulation of PGC-1α and ERRα protein during training suggests a regulatory role for these factors in adaptations of mitochondrial and metabolic gene expression. A subset of genes were transiently increased after one training session, but returned to baseline levels thereafter, which is supportive of the concept of transcriptional capacity being particularly sensitive to the onset of a new level of contractile activity. Thus, gene-specific temporal patterns of induction of mRNA expression and protein content are described. Our results illustrate the phenomenology of skeletal muscle plasticity and support the notion that transcript level adjustments, coupled to accumulation of encoded protein, underlie the modulation of skeletal muscle metabolism and phenotype by regular exercise.

  1. Reducing Staphylococcus aureus bacterial counts in a dental clinic using an Ionic Breeze air purifier: a preliminary study.

    Science.gov (United States)

    Hubar, J Sean; Pelon, William; Strother, Elizabeth A; Sicard, F Scott

    2009-01-01

    Aerosols and droplets generated by dental procedures are contaminated with blood and bacteria and represent a potential route for the transmission of disease. This study sought to determine if Ionic Breeze air purifiers are effective in collecting and destroying bacteria found in dental aerosols (such as Staphylococcus aureus). This study placed one Sharper Image Professional Series Ionic Breeze Quadra unit and one Ionic Breeze GP unit (with germicidal protection) in dental operatories within the Louisiana State University School of Dentistry. After six hours of operation, bacterial samples were collected and streaked over surfaces of petri dishes containing trypticase soy sucrose bacitracin agar that had been supplemented with 5% sheep blood. The samples were incubated at 37 degrees C for 48 hours; at that point, the microbial colonies were counted. Additional testing was performed on suspect colonies to identify S. aureus strains and to determine if any of those isolates were pathogenic with or without antibiotic resistance. The Ionic Breeze GP unit killed more than 99% of all bacteria on the stainless steel collecting blades. The non-germicidal Ionic Breeze Quadra air purifier collected numerous bacteria that were found to include some pathogenic strains of S. aureus; however, none of these were resistant to antibiotics.

  2. Modelling tidal influence on sea breezes with models of different complexity

    Directory of Open Access Journals (Sweden)

    Jana Fischereit

    2016-09-01

    Full Text Available Tides influence both the formation and development of sea breezes. The aim of this study is to investigate the tidal influence to decide which model complexity is needed to reproduce the main influence of tides in a numerical model of coastal meteorology. Two processes are considered: (a the influence of tides on sea breezes through the effect of tidal currents on the surface wind and (b the thermal influence through the flooding and drying of mudflats in the intertidal area. The processes are considered separately by representing the ocean in the non-hydrostatic mesoscale atmosphere model METRAS with different complexity, ranging from a homogeneous stationary surface to a shallow-water model coupled to METRAS with a two-way exchange of momentum. The model system is applied in a case study to the German Bight, where large mudflats exist at low tide.The results show that the main influence of tides originates from a change in the mudflat heat budget through flooding and drying. The influence of tidal currents on the surface wind is small. Therefore, we conclude that no coupled atmosphere-ocean model is needed to reproduce the main influence of tides on sea breezes in a numerical model. Instead, we suggest to use an atmosphere model which simulates the change of surface cover in the intertidal area and includes a realistic spatial sea surface temperature distribution. For this it is essential to simulate the change in surface cover with the correct timing because the results show that the atmosphere reacts very sensitively to that change.

  3. Impact of sea breeze of the sea off Goa, west coast of India

    Digital Repository Service at National Institute of Oceanography (India)

    Neetu, S.; Shetye, S.R.; Chandramohan, P.

    . OBSERVATIONS AND ANALYSIS The wind observations were made using an anemometer located on the terrace of the main building ofthe National Institute of Oceanography, Goa. The height ofthe anemometer was about 50 m above sea level. The recorded data were ten...Goa is oriented along approximately 340°. Hence the sea breeze at the height of about 50 metres above sea level recorded by the anemometer is almost along the coastline. Though this angle might have come under the influence of the shape of the building on which...

  4. Estimating the seaward extent of sea breeze from QuickSCAT scatterometry

    Digital Repository Service at National Institute of Oceanography (India)

    Aparna, M.; Shetye, S.R.; Shankar, D.; Shenoi, S.S.C.; Mehra, P.; Desai, R.G.P.

    wind vectors spaced equally along the normal to the coast, but the differing coordinates of wind vectors in the swath during each pass of the satellite does not meet this requirement. Hence, we define a set of regularly spaced 0.1C176 C2 1C176 cells (C...023107$05.00 L13601 1of4 averaged. The wind vectors thus chosen were considered equally spaced. To enhance the local signal (herein, that associated with the sea-breeze system), the mean of the wind vectors over all the cells was subtracted from each...

  5. Using Breeze for Communication and Assessment of Internships: An Exploratory Study

    Directory of Open Access Journals (Sweden)

    Kelly Wilkinson

    2008-07-01

    Full Text Available An important component of internships is the connection between students, universities and employers. “High-quality internships . . . encourage contact between faculty and students as well as develop cooperation among students,” (Implementing and Assessing Internships, 2002, 67. This “good practice” must exist to ensure good performance assessment (Implementing and assessing internships 2002. Students in an internship program were given web cameras and access to the software Breeze to use for a video journal. Focus groups were conducted to determine the students’ opinions of using this type of assessment. Students overwhelmingly preferred this type of assessment and saw the benefits of using the technology beyond the internship.

  6. Theoretical calculations of interactions between urban breezes and mountain slope winds in the presence of basic-state wind

    Science.gov (United States)

    Seo, Jaemyeong Mango; Ganbat, Gantuya; Han, Ji-Young; Baik, Jong-Jin

    2017-02-01

    Many big cities around the world are located near mountains. In city-mountain regions, thermally and topographically forced local winds are produced and they affect the transport of pollutants emitted into the urban atmosphere. A better understanding of the dynamics of thermally and topographically forced local winds is necessary to improve the prediction of local winds and to cope with environmental problems. In this study, we theoretically examine the interactions of urban breezes with mountain slope winds in the presence of basic-state wind within the context of the response of a stably stratified atmosphere to prescribed thermal and mechanical forcing. The interactions between urban breezes and mountain slope winds are viewed through the linear superposition of individual analytical solutions for urban thermal forcing, mountain thermal forcing, and mountain mechanical forcing. A setting is considered in which a city is located downwind of a mountain. In the nighttime, in the mountain-side urban area, surface/near-surface horizontal flows induced by mountain cooling and mountain mechanical forcing cooperatively interact with urban breezes, resulting in strengthened winds. In the daytime, in the urban area, surface/near-surface horizontal flows induced by mountain heating are opposed to urban breezes, giving rise to weakened winds. It is shown that the degree of interactions between urban breezes and mountain slope winds is sensitive to mountain height and basic-state wind speed. Particularly, a change in basic-state wind speed affects not only the strength of thermally and mechanically induced flows (internal gravity waves) but also their vertical wavelength and decaying rate. The examination of a case in a setting in which a city is located upwind of a mountain reveals that basic-state wind direction is an important factor that significantly affects the interactions of urban breezes with mountain slope winds.

  7. Physical training and weight loss in dogs lead to transcriptional changes in genes involved in the glucose-transport pathway in muscle and adipose tissues

    DEFF Research Database (Denmark)

    Herrera Uribe, Juber; Vitger, Anne Désiré; Ritz, Christian

    2016-01-01

    on calorie restriction and physical training (FD group, n = 5) or calorie restriction alone (DO group, n = 7). mRNA expression of 12 genes and six microRNAs were investigated using quantitative real-time PCR (qPCR). In the FD group, FOXO1 and RAC1 were expressed at lower levels in adipose tissue, whereas...... and two microRNAs were significantly downregulated (NRF2, RAC1, ESRRA, AKT2, PGC1a and mir-23; P calorie restriction causes regulation of several metabolic genes in both tissues. The mild exercise, incorporated into this study design, was sufficient to elicit transcriptional...

  8. Numerical simulation of sea breeze characteristics observed at tropical coastal site, Kalpakkam

    Indian Academy of Sciences (India)

    P Jamima; J Lakshminarasimhan

    2004-06-01

    Sea breeze characteristics around Kalpakkam tropical coastal site are studied using an Advanced Regional Prediction System (ARPS) mesoscale model, which is non-hydrostatic, compressible atmospheric prediction model following the terrain coordinate system. Various options such as surface physics, atmospheric radiation physics, Coriolis force, microphysics, cumulus parameterization and 1.5 level TKE closure scheme for diffusion are included in the model. A joint meteorological field experiment was carried out by IITM-Pune and IGCAR at Kalpakkam by deploying state-of-the-art sensors and tether balloon systems for observing the height profiles of meteorological parameters. The data obtained from the field experiment are used here to compare the results from numerical simulations. From the simulated results, it is seen that duration of the sea breeze is 6 hours which agrees well with the observations. The height of the Thermal Internal Boundary Layer (TIBL) is also simulated from the vertical profiles of potential temperature. Simulated wind speed and wind directions are compared with the 50m tower data and potential temperature profiles are compared with the kytoon data. Results are in good agreement with the observed values except during night time wherein a small difference is seen in the wind speed.

  9. Impact of land-sea breezes at different scales on the diurnal rainfall in Taiwan

    Science.gov (United States)

    Huang, Wan-Ru; Wang, Shih-Yu

    2014-10-01

    The formation mechanism of diurnal rainfall in Taiwan is commonly recognized as a result of local forcings involving solar thermal heating and island-scale land-sea breeze (LSB) interacting with orography. This study found that the diurnal variation of the large-scale circulation over the East Asia-Western North Pacific (EAWNP) modulates considerably the diurnal rainfall in Taiwan. It is shown that the interaction between the two LSB systems—the island-scale LSB and the large-scale LSB over EAWNP—facilitates the formation of the early morning rainfall in western Taiwan, afternoon rainfall in central Taiwan, and nighttime rainfall in eastern Taiwan. Moreover, the post-1998 strengthening of a shallow, low-level southerly wind belt along the coast of Southeast China appears to intensify the diurnal rainfall activity in Taiwan. These findings reveal the role of the large-scale LSB and its long-term variation in the modulation of local diurnal rainfall.

  10. Observed and simulated sea breeze characteristics over Rayong coastal area, Thailand

    Science.gov (United States)

    Phan, Tung Thanh; Manomaiphiboon, Kasemsan

    2012-05-01

    This work presents the detailed characterization of sea breeze (SB) over the Rayong coastal area, one of the most rapidly developed and highly industrialized areas during the last decade in Thailand, using observation data analysis and fine-resolution (2 km) mesoscale meteorological modeling with incorporation of new land cover and satellite-derived vegetation fraction data sets. The key characteristics considered include frequency of SB occurrence, sea-breeze day (SBD) identification, degree of inland penetration, and boundary layer development. It was found that SBs occur frequently in the winter due mainly to relatively large land-sea temperature contrasts and minimally in the wet season. Monthly mean SB onset and cessation times are at around 12-15 local time (LT) and 18-21 LT, respectively, and its strength peaks during the early- to mid-afternoon. Monthly SB hodographs generally exhibit clockwise rotations, and SB inland penetration (at PCD-T tower) ranges widely with the monthly means of 25-55 km from the coast. Mesoscale MM5 modeling was performed on two selected SBDs (13 January and 16 March 2006), on which the SBs are under weak and onshore strong influences from background winds, respectively. Simulated near-surface winds and temperature were found to be in fair-to-acceptable agreement with the observations. The SB circulation along the Rayong coast is clearly defined with a return flow aloft and a front on 13 January, while it is enhanced by the onshore background winds on 16 March. Another SB along the Chonburi coast also develops separately, but their fronts merge into one in the mid-afternoon, resulting in large area coverage by the SB. Simulated planetary boundary layer height over the land area is significantly affected by a thermal internal boundary layer (TIBL) induced by an SB, which is found to be low near the coast and increases toward the front (up to 800-1,000 m along the Rayong coast).

  11. An Israeli haboob: Sea breeze activating local anthropogenic dust sources in the Negev loess

    Science.gov (United States)

    Crouvi, Onn; Dayan, Uri; Amit, Rivka; Enzel, Yehouda

    2017-02-01

    Meso-scale weather systems, such as convective haboobs, are considered to be an important dust generation mechanism. In Israel, however, rather than of meso-scale weather systems, most dust storms are generated by synoptic-scale systems, originating from Sahara and Arabia. Consequently, only distal sources of suspended and deposited dust in Israel are currently reported. Here we report the first detailed study on the merging of synoptic- and meso-scale weather systems leading to a prominent dust outbreak over the Negev, Israel. During the afternoon of May 2nd, 2007, a massive dust storm covered the northern Negev, forming a one kilometer high wall of dust. The haboob was associated with PM10 concentrations of 1000-1500 μg m-3 that advanced at a speed of 10-15 m s-1 and caused temporary closure of local airports. In contrast to most reported haboobs, this one was generated by a sea breeze front acting as a weak cold front enhanced by a cold core cyclone positioned over Libya and Egypt. The sea breeze that brought cold and moist marine air acted as a gravity current with strong surface winds. The sources for the haboob were the loessial soils of the northwestern Negev, especially agricultural fields that were highly disturbed in late spring to early summer. Such surface disturbance is caused by agricultural and/or intensive grazing practices. Our study emphasizes the importance of local dust sources in the Negev and stresses loess recycling as an important process in contemporary dust storms over Israel.

  12. The influence of the summer sea breeze on thermal comfort in Funchal (Madeira). A contribution to tourism and urban planning.

    Energy Technology Data Exchange (ETDEWEB)

    Lopes, Antonio; Lopes, Sergio; Joao Alcoforado, Maria [Univ. de Lisboa (Portugal). Centro der Estudos Geograficos; Matzarakis, Andreas [Freiburg Univ. (Germany). Meteorological Inst.

    2011-10-15

    Tourism plays a crucial role for the development of coastal areas. Despite the mildness of Madeira's climate, very hot days can occur during summer, a situation to which most tourists from northern Europe (the majority of foreign tourists) are poorly adapted. As sea breezes strongly contribute to moderate heat stress in urban environments, their influence on the thermal comfort on the island has been studied. Sea breezes occurred on 84 % of the days during the period under study (May to October 2006). They usually start around 09:30 h and end after 22:00 h, with an average duration of about 12:50 hours and a mean velocity of 2.9 m/s. Physiologically Equivalent Temperature (PET) was used to evaluate the thermal comfort of a sample of days during the summer of 2006. It was concluded that most of the sites in the city are ''slightly comfortable'' during normal days with sea breeze, but only shore sites and the highest green areas offer some comfort during hot days. Inside the city, the thermal perception is generally ''hot'' and strong heat stress can occur. As sea breezes are important to mitigate heat stress, some basic guidelines were presented: urban planners should take advantage of this wind system avoiding dense construction near the shoreline that would act as a barrier to the renewal of the air inside the city. In terms of tourism, planners and local authorities should provide solutions to mitigate the negative effects during hot periods, creating a system to warn and relocate more vulnerable tourists to places near the shore line, to the mountains, to gardens and air-conditioned buildings. In combination with other components (beauty of the island, gastronomy, cultural values and safety), climate information can be a factor of attractiveness to tourists. (orig.)

  13. Air pollutant transport in a coastal environment. Part 1: Two-dimensional simulations of sea-breeze and mountain effects

    Science.gov (United States)

    Lu, Rong; Turco, Richard P.

    1994-01-01

    Over the southern California coastal region, observations of the vertical distributions of pollutants show that maximum concentrations can occur within temperature inversion layers well above the surface. A mesoscale model is used to study the dynamical phenomena that cause such layers, including sea breezes and mountain flows, and to study the characteristics of air pollutant transport in a coastal environment capped by a temperature inversion. The mathematical and physical structure of the model is described. Two-dimensional simulations corresponding to four configurations of coastal plains and mountains are discussed. The simulations reveal that pollutant transport over a coastal plain is strongly influenced by the topographic configuration, including the height of coastal mountains and their distance from the coastline. Sea breezes induced by land-sea thermal contrasts, as well as upslope winds induced along mountain flanks, both create vertical transport that can lead to the formation of elevated pollution layers. The sea-breeze circulation generates pollution layers by undercutting the mixed layer and lofting pollutants into the stable layer. Heating of mountain slopes acts to vent pollutants above the mountain ridge during the day; during the evening, pollutants can be injected directly into the inversion layer from the decaying upslope flows. In a land-sea configuration with mountains close to the coastline, the sea breeze and heated-mountain flow are strongly coupled. In the afternoon, this interaction can produce upslope flow from which polluted air is detrained into the inversion layer as a return circulation. When the mountains lie farther inland, however, pollutants may be trapped aloft when the mixed layer stabilizes in the late afternoon. As the nocturnal boundary layer forms over the coast in the evening, polluted mixed-layer air is effectively left behind in the inversion layer. In the Los Angeles Basin, the formation mechanism for elevated

  14. Evaluation of weather research and forecasting model parameterizations under sea-breeze conditions in a North Sea coastal environment

    Science.gov (United States)

    Salvador, Nadir; Reis, Neyval Costa; Santos, Jane Meri; Albuquerque, Taciana Toledo de Almeida; Loriato, Ayres Geraldo; Delbarre, Hervé; Augustin, Patrick; Sokolov, Anton; Moreira, Davidson Martins

    2016-12-01

    Three atmospheric boundary layer (ABL) schemes and two land surface models that are used in the Weather Research and Forecasting (WRF) model, version 3.4.1, were evaluated with numerical simulations by using data from the north coast of France (Dunkerque). The ABL schemes YSU (Yonsei University), ACM2 (Asymmetric Convective Model version 2), and MYJ (Mellor-Yamada-Janjic) were combined with two land surface models, Noah and RUC (Rapid Update Cycle), in order to determine the performances under sea-breeze conditions. Particular attention is given in the determination of the thermal internal boundary layer (TIBL), which is very important in air pollution scenarios. The other physics parameterizations used in the model were consistent for all simulations. The predictions of the sea-breeze dynamics output from the WRF model were compared with observations taken from sonic detection and ranging, light detection and ranging systems and a meteorological surface station to verify that the model had reasonable accuracy in predicting the behavior of local circulations. The temporal comparisons of the vertical and horizontal wind speeds and wind directions predicted by the WRF model showed that all runs detected the passage of the sea-breeze front. However, except for the combination of MYJ and Noah, all runs had a time delay compared with the frontal passage measured by the instruments. The proposed study shows that the synoptic wind attenuated the intensity and penetration of the sea breeze. This provided changes in the vertical mixing in a short period of time and on soil temperature that could not be detected by the WRF model simulations with the computational grid used. Additionally, among the tested schemes, the combination of the localclosure MYJ scheme with the land surface Noah scheme was able to produce the most accurate ABL height compared with observations, and it was also able to capture the TIBL.

  15. Identifying a Sea Breeze Circulation Pattern Over the Los Angeles Basin Using Airborne In Situ Carbon Dioxide Measurements

    Science.gov (United States)

    Brannan, A. L.; Schill, S.; Trousdell, J.; Heath, N.; Lefer, B. L.; Yang, M. M.; Bertram, T. H.

    2014-12-01

    The Los Angeles Basin in Southern California is an optimal location for a circulation study, due to its location between the Pacific Ocean to the west and the Santa Monica and San Gabriel mountain ranges to the east, as well as its booming metropolitan population. Sea breeze circulation carries air at low altitudes from coastal to inland regions, where the air rises and expands before returning back towards the coast at higher altitudes. As a result, relatively clean air is expected at low altitudes over coastal regions, but following the path of sea breeze circulation should increase the amount of anthropogenic influence. During the 2014 NASA Student Airborne Research Program, a highly modified DC-8 aircraft completed flights from June 23 to 25 in and around the LA Basin, including missed approaches at four local airports—Los Alamitos and Long Beach (coastal), Ontario and Riverside (inland). Because carbon dioxide (CO2) is chemically inert and well-suited as a conserved atmospheric tracer, the NASA Langley Atmospheric Vertical Observations of CO2 in the Earth's Troposphere (AVOCET) instrument was used to make airborne in situ carbon dioxide measurements. Combining measured wind speed and direction data from the aircraft with CO2 data shows that carbon dioxide can be used to trace the sea breeze circulation pattern of the Los Angeles basin.

  16. Interstellar neutral helium in the heliosphere from IBEX observations. IV. Flow vector, Mach number, and abundance of the Warm Breeze

    CERN Document Server

    Kubiak, M A; Bzowski, M; Sokol, J M; Fuselier, S A; Galli, A; Heirtzler, D; Kucharek, H; Leonard, T W; Moebius, D J McComas E; Park, J; Schwadron, N A; Wurz, P

    2016-01-01

    With the velocity vector and temperature of the pristine interstellar neutral (ISN) He recently obtained with high precision from a coordinated analysis summarized by McComas et al.2015b, we analyzed the IBEX observations of neutral He left out from this analysis. These observations were collected during the ISN observation seasons 2010---2014 and cover the region in the Earth's orbit where the Warm Breeze persists. We used the same simulation model and a very similar parameter fitting method to that used for the analysis of ISN He. We approximated the parent population of the Warm Breeze in front of the heliosphere with a homogeneous Maxwell-Boltzmann distribution function and found a temperature of $\\sim 9\\,500$ K, an inflow speed of 11.3 km s$^{-1}$, and an inflow longitude and latitude in the J2000 ecliptic coordinates $251.6^\\circ$, $12.0^\\circ$. The abundance of the Warm Breeze relative to the interstellar neutral He is 5.7\\% and the Mach number is 1.97. The newly found inflow direction of the Warm Bree...

  17. Atmospheric electrical field measurements near a fresh water reservoir and the formation of the lake breeze

    Directory of Open Access Journals (Sweden)

    Francisco Lopes

    2016-06-01

    Full Text Available In order to access the effect of the lakes in the atmospheric electrical field, measurements have been carried out near a large man-made lake in southern Portugal, the Alqueva reservoir, during the ALqueva hydro-meteorological EXperiment 2014. The purpose of these conjoint experiments was to study the impact of the Alqueva reservoir on the atmosphere, in particular on the local atmospheric electric environment by comparing measurements taken in the proximity of the lake. Two stations 10 km apart were used, as they were located up- and down-wind of the lake (Amieira and Parque Solar, respectively, in reference to the dominant northwestern wind direction. The up-wind station shows lower atmospheric electric potential gradient (PG values than the ones observed in the down-wind station between 12 and 20 UTC. The difference in the atmospheric electric PG between the up-wind and the down-wind station is ~30 V/m during the day. This differential occurs mainly during the development of a lake breeze, between 10 and 18 UTC, as a consequence of the surface temperature gradient between the surrounding land and the lake water. In the analysis presented, a correlation is found between the atmospheric electric PG differences and both wind speed and temperature gradients over the lake, thus supporting the influence of the lake breeze over the observed PG variation in the two stations. Two hypotheses are provided to explain this observation: (1 The air that flows from the lake into the land station is likely to increase the local electric conductivity through the removal of ground dust and the transport of cleaner air from higher altitudes with significant light ion concentrations. With such an increase in conductivity, it is expected to see a reduction of the atmospheric electric PG; (2 the resulting air flow over the land station carries negative ions formed by wave splashing in the lake's water surface, as a result of the so-called balloelectric effect

  18. Evaluation of the atmospheric model WRF on the Qatar peninsula for a converging sea-breeze event

    Science.gov (United States)

    Balan Sobhana, Sandeepan; Nayak, Sashikant; Panchang, Vijay

    2016-04-01

    Qatar, a narrow peninsula covering an area of 11437 sq km, extends northwards into the Arabian Gulf for about 160km and has a maximum width of 88km. The convex shape of the coast-line and narrowness of the peninsula results in the Qatar region experiencing complex wind patterns. The geometry is favorable for formation of the land-sea breeze from both coastal sides of the peninsula. This can lead to the development of sea breeze convergence zones in the middle of the country. Although circulations arising from diurnal thermal contrast of land and water are amongst most intensively studied meteorological phenomena, there is no reported study for the Qatar peninsula and very few studies are reported for the Arabian Gulf region as whole. It is necessary to characterize the wind field for applications such as assessing air pollution, renewable energy etc. A non-hydrostatic mesoscale model, Weather Research and Forecast (WRF) with a nested high resolution grid permits the investigation of such fine scale phenomena. Data from eighteen land based Automated Weather Stations (AWS) and two offshore buoys deployed and maintained by the Qatar Meteorological Department were analyzed. Based on the analysis a clear case of sea breeze convergence were seen on 18 September 2015. Model simulations were used to investigate the synoptic conditions associated with the formation of this event. The season is characterized by week ambient north westerly wind over the Arabian Gulf. The WRF model performance is validated using observed in-situ data. Model simulations show that vertical extent of sea breeze cell was up to 1 km and the converging sea breeze regions were characterized with high vertical velocities. The WRF simulation also revealed that with high resolution, the model is capable of reproducing the fine scale patterns accurately. The error of predictions in the inner domain (highest resolution) are found to be relatively lower than coarse resolution domain. The maximum wind speed

  19. Effect of the catalyzing combustion of coke breeze on the energy saving in sinter process

    Institute of Scientific and Technical Information of China (English)

    2001-01-01

    The mechanism of additive ZC-1 for catalyzing combustion of coke breeze in sintering of iron one fines was studied by using X-ray Diffraction, TGA and GC (Gas Chromatographer), by which energy saving was achieved on sinter pot test. The results show that the distance between cleavage planes is enlarged and the π-electrons are re-distibuted because of the insertion of cations/molecules of ZC-1 into the cleavage planes of carbon , resulting in the weakening of C-C bond, improving the adsorption of oxygen and lowering the activation energy of gasification of carbon from 25.8 kJ/mol to 18.9 kJ/mol , and the burning rate being increased and combustion residual reduced, all of which can lead to lower solid fuel consumption . Compared with the sintering test without addition of additives , the solid fuel consumption is reduced by 16.0%, meanwhile, the rate of finished sinter, the output of finished sinter and the tumbling index are increased by 2.03%, 7.0% and 3.71%, respectively, with incorporating 0.2% of additive ZC-1 in the sintering mixture, which is in agreement with the catalyzing mechanism of ZC-1.

  20. Fire Whirl Evolution Observed during a Valley Wind-Sea Breeze Reversal

    Directory of Open Access Journals (Sweden)

    Daisuke Seto

    2011-01-01

    Full Text Available This observational study documented the atmospheric environment of a prescribed fire conducted in a narrow valley when a small fire whirl developed during a mesoscale wind reversal. Based on analysis of in situ meteorological measurements, it is hypothesized that the fire whirl formed due to the presence of strong vertical wind shear caused by the interaction of a sea breeze front with a weaker up-valley wind. Vorticity generated by the interaction of the wind shear and the fire front was estimated to be ~0.2 s−1. Peak turbulence kinetic energy was caused by the wind shear rather than the buoyancy generated by the fire front. It was also found that the convective Froude number itself may not be sufficient for fire whirl prediction since it is less relevant to the near-surface boundary-layer turbulence generated by environmental wind shear. Observations from this case study indicate that even low-intensity prescribed fires can result in the formation of fire whirls due to mesoscale changes in the ambient atmospheric environment.

  1. Uma teoria termodinâmica para brisas: teste utilizando simulações numéricas A thermodynamic theory for breezes: test using numeric simulations

    Directory of Open Access Journals (Sweden)

    Clênia Rodrigues Alcântara

    2008-03-01

    Full Text Available O objetivo deste trabalho foi testar uma teoria termodinâmica em brisas marítimas-terrestres acopladas com brisas de vale-montanha através de simulações numéricas tridimensionais em uma região da costa leste do Nordeste Brasileiro, considerando a presença e a ausência da topografia. Embora o contraste de temperatura entre as superfícies seja importante na formação da brisa, a eficiência termodinâmica é fundamental na determinação da sua intensidade. Tem-se que a inclinação faz com que a diferença de pressão entre dois pontos fique maior durante o dia e menor durante a noite contribuindo para a formação de brisas marítimas mais intensas e de brisas terrestres menos intensas, respectivamente. A máxima queda de pressão ocorre por volta de três horas antes da máxima intensidade da brisa. Isso porque grande parte da energia disponibilizada para as circulações é gasta para vencer dissipação, principalmente, no período diurno, quando esses processos são realmente efetivos. Do ponto de vista puramente termodinâmico a inclinação da montanha atua para intensificar a brisa durante o dia e para enfraquecê-la durante a noite.In this work we test a thermodynamic theory for sea-land breeze coupled with valley-mountain breeze through tri-dimensional numeric simulations. We verify the presence of such a breeze in Northeast Brazil's east coast and perform experiments with and without the topography of the region. Although the temperature contrast between two surfaces is important in forming the breezes, the thermodynamic efficiency is a key parameter for the breeze intensity. The presence of the slope causes the pressure difference between two points to increase during the day and to decrease during the night. This contributes for more intense daytime breeze and less intense nighttime ones. The maximum pressure drop occurs about three hours prior the maximum intensity of the sea breeze. This is because most of the available

  2. Surface current dynamics under sea breeze conditions observed by simultaneous HF radar, ADCP and drifter measurements

    Science.gov (United States)

    Sentchev, Alexei; Forget, Philippe; Fraunié, Philippe

    2017-02-01

    Ocean surface boundary layer dynamics off the southern coast of France in the NW Mediterranean is investigated by using velocity observations by high-frequency (HF) radars, surface drifting buoys and a downward-looking drifting acoustic Doppler current profiler (ADCP). The analysis confirms that velocities measured by HF radars correspond to those observed by an ADCP at the effective depth z f = k -1, where k is wavenumber of the radio wave emitted by the radar. The radials provided by the radars were in a very good agreement with in situ measurements, with the relative errors of 1 and 9 % and root mean square (RMS) differences of 0.02 and 0.04 m/s for monostatic and bistatic radar, respectively. The total radar-based velocities appeared to be slightly underestimated in magnitude and somewhat biased in direction. At the end of the survey period, the difference in the surface current direction, based on HF radar and ADCP data, attained 10°. It was demonstrated that the surface boundary layer dynamics cannot be reconstructed successfully without taking into the account velocity variation with depth. A significant misalignment of ˜30° caused by the sea breeze was documented between the HF radar (HFR-derived) surface current and the background current. It was also found that the ocean response to a moderate wind forcing was confined to the 4-m-thick upper layer. The respective Ekman current attained the maximum value of 0.15 m/s, and the current rotation was found to be lagging the wind by approximately 40 min, with the current vector direction being 15-20° to the left of the wind. The range of velocity variability due to wind forcing was found comparable with the magnitude of the background current variability.

  3. Interaction Of Mesoscale Convective Systems With The Land - Sea Breezes Along The Guinea Coast Of West Africa

    Science.gov (United States)

    Coulibaly, A.; Omotosho, B. J.; Sylla, M. B.; Fink, A. H.

    2015-12-01

    1-3 hourly METARS/SYNOP observation data of wind (speed and direction), air temperature over land, covering the period 1983-2012, were used in order to characterize land-sea breezes (LSB) over five coastline stations (Abidjan, Accra, Lomé, Cotonou and Ikeja-Lagos) in the Guinean Coast, West Africa. In additional to this, 8 years (2003-2010) sea surface temperature (SST) data from adjacent Atlantic Ocean and monthly METARS/SYNOP observation data of precipitation (1983-2012) were also used. Based on wind speed and direction, the wind roses of all months over all stations have been plotted. These wind roses reveal that, globally, the northerly winds occurred rarely for the months of February, March, and April at Cotonou (Benin Republic).This is seen for other stations (Lomé and Accra). The night/morning time northerly winds appear at all stations, except Accra where there is no northerly wind or is very weak. According to the stations basis, the period of that night/morning northerly winds varies, except in summer period (July - September), where the normal s/w monsoon winds can weaken override at all stations. Set criteria based on the diurnal reversal of wind direction, and the thermal gradient necessary to drive the wind circulation, was used to identify land-sea breeze days. On station-wide basis, sea-breezes occur, at least all period of year, but less frequent in the summer months, where the minimum value of monthly mean temperature gradient is observed for selected stations.

  4. Physical training and weight loss in dogs lead to transcriptional changes in genes involved in the glucose-transport pathway in muscle and adipose tissues.

    Science.gov (United States)

    Herrera Uribe, Juber; Vitger, Anne D; Ritz, Christian; Fredholm, Merete; Bjørnvad, Charlotte R; Cirera, Susanna

    2016-02-01

    Obesity is a worldwide problem in humans and domestic animals. Interventions, including a combination of dietary management and exercise, have proven to be effective for inducing weight loss in humans. In companion animals, the role of exercise in the management of obesity has received relatively little attention. The aim of the present study was to investigate changes in the transcriptome of key energy metabolism genes in muscle and adipose tissues in response to diet-induced weight loss alone, or combined with exercise in dogs. Overweight pet dogs were enrolled on a weight loss programme, based on calorie restriction and physical training (FD group, n = 5) or calorie restriction alone (DO group, n = 7). mRNA expression of 12 genes and six microRNAs were investigated using quantitative real-time PCR (qPCR). In the FD group, FOXO1 and RAC1 were expressed at lower levels in adipose tissue, whereas ESRRA and AKT2 were more highly expressed in muscle, when compared with the DO group. Comparing expression before and after the intervention, in the DO group, nine genes and three microRNAs showed significant altered expression in adipose tissue (PPARG, ADIPOQ and FOXO1; P ESRRA, AKT2, PGC1a and mir-23; P < 0.001) in muscle. Thus, calorie restriction causes regulation of several metabolic genes in both tissues. The mild exercise, incorporated into this study design, was sufficient to elicit transcriptional changes in adipose and muscle tissues, suggesting a positive effect on glucose metabolism. The study findings support inclusion of exercise in management of canine obesity.

  5. Review of: Maddie Breeze: Seriousness and Women’s Roller Derby. Gender, Organization and Ambivalence. London u.a.: Palgrave Macmillan 2015.

    Directory of Open Access Journals (Sweden)

    Wiebke Dierkes

    2016-12-01

    Full Text Available In her autoethnographic study in the field of sports sociology Maddie Breeze presents a captivating analysis of the roller derby scene. Starting from empirical material she develops a new sociological concept of 'seriousness' and '(wanting-to-be-taken-seriously' which contrasts with established, linear and normative models – both are re-conceived as a contradictory and ambivalent practice. Thus, Breeze is able to show how new forms of identity and of organization can be successfully established in processes of transformation, without having to negate or dismiss previous ones. A highly recommendable book, not only for sports sociologists.

  6. Utilizing TRMM to Analyze Sea Breeze Thunderstorm Patterns During El Nino Southern Oscillations and Their Effects upon Available Fresh Water for South Florida Agricultural Planning and Management

    Science.gov (United States)

    Cooley, Clayton; Billiot, Amanda; Lee, Lucas; McKee, Jake

    2010-01-01

    Water is in high demand for farmers regardless of where you go. Unfortunately, farmers in southern Florida have fewer options for water supplies than public users and are often limited to using available supplies from surface and ground water sources which depend in part upon variable weather patterns. There is an interest by the agricultural community about the effect weather has on usable surface water, however, research into viable weather patterns during La Nina and El Nino has yet to be researched. Using rainfall accumulation data from NASA Tropical Rainfall Measurement Mission (TRMM) satellite, this project s purpose was to assess the influence of El Nino and La Nina Oscillations on sea breeze thunderstorm patterns, as well as general rainfall patterns during the summer season in South Florida. Through this research we were able to illustrate the spatial and temporal variations in rainfall accumulation for each oscillation in relation to major agricultural areas. The study period for this project is from 1998, when TRMM was first launched, to 2009. Since sea breezes in Florida typically occur in the months of May through October, these months were chosen to be the months of the study. During this time, there were five periods of El Nino and two periods of La Nina, with a neutral period separating each oscillation. In order to eliminate rainfall from systems other than sea breeze thunderstorms, only days that were conducive to the development of a sea breeze front were selected.

  7. A numerical study of sea breeze circulation observed at a tropical site Kalpakkam on the east coast of India, under different synoptic flow situations

    Indian Academy of Sciences (India)

    C V Srinivas; R Venkatesan; K M Somayaji; A Bagavath Singh

    2006-10-01

    A simulation study of the sea breeze circulation and thermal internal boundary layer (TIBL) characteristics has been carried out at the tropical site Kalpakkam on the east coast of India, for operational atmospheric dispersion prediction. The community based PSU/NCAR MM5 Mesoscale meteorological model is used for the study. Three cases on typical days in summer (24 May 2003), southwest (SW) monsoon (1 July 2001) winter season (2 February 2003) with different largescale flow pattern are studied. The MM5 model is used with 3 nested domains with horizontal grid resolutions 18 km, 6 km and 2 km and 26 vertical levels. The model is integrated for 24 hours in the above cases with initial and boundary conditions taken from NCEP-FNL analyses data. Observations of 10 meteorological stations and coastal boundary layer experiments conducted at Kalpakkam are used for comparison and validation of the simulation. The characteristics of simulated sea breeze and TIBL at Kalpakkam are seen to vary in the above cases according to the prevailing large-scale winds and surface fluxes. The sea breeze circulation is seen to develop early with larger strength and inland propagation in the summer case under the influence of moderate synoptic wind and strong heating conditions than in the SW monsoon and winter cases. The horizontal and vertical extents of TIBL are found to be larger in the summer case than in other cases. Although model parameters agree in general with observations, all the fine features are not clearly captured and some slowness in model sea breeze development is also seen. The results indicate the need to improve i) the initial conditions by assimilation of available surface/upper air observations to reduce model bias and ii) surface net radiation parameterisation. The model could predict the essential features of the local circulation and further improvement is expected with better initial condition data and incorporation of more realistic surface data.

  8. The role of refinery flaring events and bay breezes on a high surface ozone episode during the Houston, Texas DISCOVER-AQ field campaign

    Science.gov (United States)

    Loughner, C.; Follette-Cook, M. B.; Fried, A.; Pickering, K. E.

    2015-12-01

    The highest observed surface ozone concentrations in the Houston metropolitan area in 2013 occurred on September 25, which coincided with the Texas DISCOVER-AQ (Deriving Information on Surface Conditions from Column and Vertically Resolved Observations Relevant to Air Quality) field campaign. Surface ozone was elevated throughout the Houston metropolitan area with maximum 8-hour average ozone peaking along the western shore of Galveston Bay, reaching 124 ppbv, almost 50 ppbv above the current EPA standard of 75 ppbv. The NASA P-3B aircraft observed plumes from refinery flares west and northwest of Galveston Bay that were transported over the water. Continental air pollution from the north was transported into the Houston metropolitan area where it mixed with locally generated emissions. A bay breeze circulation formed causing pollutants that were transported out over the water in the morning to recirculate back inland where they mixed with freshly emitted pollution near the bay breeze convergence zone. The highest surface ozone concentrations were reported near the bay breeze front. This ozone episode will be presented using measurements made during the DISCOVER-AQ field campaign and a CMAQ model simulation with integrated source apportionment, which tracks the contribution of emissions source groups and regions on ozone concentrations.

  9. The role of bay breezes and regional transport on a high surface ozone episode during the Houston, Texas DISCOVER-AQ field campaign

    Science.gov (United States)

    Loughner, C.; Follette-Cook, M. B.; Pickering, K. E.; Estes, M. J.

    2014-12-01

    The highest observed surface ozone concentrations in the Houston metropolitan area in 2013 occurred on September 25, which coincided with the Texas DISCOVER-AQ (Deriving Information on Surface Conditions from Column and Vertically Resolved Observations Relevant to Air Quality) field campaign. Surface ozone was elevated throughout the Houston metropolitan area. Maximum 8-hour average ozone peaked along the western shore of Galveston Bay, reaching 124 ppbv, almost 50 ppbv above the current EPA standard of 75 ppbv, at La Porte Sylvan Beach. Continental air pollution from the north and northeast was transported into the Houston metropolitan area where it mixed with locally generated emissions. A bay breeze circulation formed causing pollutants that were transported out over the water in the morning to recirculate back inland where they mixed with freshly emitted pollution near the bay breeze convergence zone. The highest surface ozone concentrations were reported near the bay breeze front at La Porte Sylvan Beach. This ozone episode will be presented using measurements made during the DISCOVER-AQ field campaign and WRF and CMAQ model simulations.

  10. Gene Transcription and Virulence Potential of Listeria monocytogenes Strains After Exposure to Acidic and NaCl Stress

    DEFF Research Database (Denmark)

    Olesen, Inger; Vogensen, Finn Kvist; Jespersen, Lene

    2009-01-01

    response and invasion (clpC, clpP, inlA, inlB, prfA, and sigB) were induced in 4140. Long-term adaptation of EGD-e to NaCl stress increased transcription of genes important for the intracellular life cycle (actA, hly, iap, inlA, inlB, plcA, plcB, and prfA), while few changes were observed for 4140...

  11. Severe Weather Caused by Heat Island and Sea Breeze Effects in the Metropolitan Area of São Paulo, Brazil

    Directory of Open Access Journals (Sweden)

    Felipe Vemado

    2016-01-01

    Full Text Available The Metropolitan Area of São Paulo (MASP is one of the most populated regions of the planet with one of the largest impervious regions as well. This research work aims to characterize MASP heat island (HI effect and its interaction with the local sea breeze (SB inflow in rainfall amounts and deep convection. The combined SB-HI produces direct circulation over the MASP and produces severe weather and socioeconomic impacts. All SB-HI episodes between 2005 and 2008 are identified and analyzed with surface and upper air measurements, weather radar, and satellite data. The current work indicates that intense SB-HI episodes are related to air and dew point temperatures above 30°C and 20°C, respectively, right after the passage of the SB front over MASP. Results indicate that the precipitation related to SB-HI episodes is up to 600 mm or about four times higher than that in rural or less urbanized areas in its surroundings. Measurements indicate that 74% of SB-HI episodes are related to NW winds in earlier afternoon hours. Moving cold fronts in southern Brazil tend to intensify the SB-HI circulation in MASP. A conceptual model of these patterns is presented in this paper.

  12. A modeling study of convection initiation prior to the merger of a sea-breeze front and a gust front

    Science.gov (United States)

    Abulikemu, Abuduwaili; Xu, Xin; Wang, Yuan; Ding, Jinfeng; Zhang, Shushi; Shen, Wenqiang

    2016-12-01

    Convection initiation (CI) prior to the merger of a sea-breeze front (SBF) with a gust front (GF) in North China is investigated using a real-data Weather Research and Forecasting (WRF) simulation with a high resolution of 444.4 m. The overall evolution of the GF and SBF is well reproduced by the simulation. The GF was produced by the decaying convective storm over northern Beijing, while the SBF came from the Bohai Sea. Several convective cells were generated between the two fronts even though they were still about 25-30 km far away from each other. During the development of these cells, the low-level convergence and conditional instability averaged within the intermediate area between the two fronts were enhanced significantly, both of which favored the initiation of convection. Vertical momentum budgets were conducted in the intermediate area as well as along the backward trajectories of parcels within a selected convective cell. The vertical acceleration was decomposed into dynamic and buoyant components, respectively. The diagnostic results showed that the dynamic acceleration dominated in the low level, while buoyant acceleration became evident only when the parcel reached a high altitude above 2 km. Therefore the dynamic forcing appeared to be more relevant to CI. The dynamic acceleration was further decomposed into four terms based on anelastic approximation. The positive dynamic acceleration was mainly caused by fluid extension associated with the low-level convergence, while fluid twisting in the vertical contributed negatively to the dynamic acceleration. The other two terms related to horizontal curvature and height variation of density were negligibly small.

  13. Identification and interpretation of representative ozone distributions in association with the sea breeze from different synoptic winds over the coastal urban area in Korea.

    Science.gov (United States)

    Hwang, Mi-Kyoung; Kim, Yoo-Keun; Oh, In-Bo; Lee, Hwa Woon; Kim, Cheol-Hee

    2007-12-01

    To aid the studies of long-term impact assessment of cumulative ozone (O3) exposures, the representative 8-hr O3 pollution patterns have been identified over the Greater Seoul Area (GSA) in Korea. Principal component analysis and two-stage clustering techniques were used to identify the representative O3 patterns, and numerical and observational analyses were also used to interpret the identified horizontal distribution patterns. The results yielded three major O3 distribution patterns, and each of the three patterns was found to have strong correlations with local and synoptic meteorological conditions over the GSA. For example, pattern 1, accounting for 46% of O3 concentration distributions, mostly occurred under relatively weak westerly synoptic winds. The predominant features of this pattern were infrequent high O3 levels but a distinct gradient of O3 concentration from the western coastal area to the eastern inland area that was mainly induced by the local sea breeze. Pattern 2, accounting for 31% of O3 concentration distributions, was found with higher O3 levels in the western coastal area but lower in the eastern inland area. This is due to the modified sea breeze under the relatively stronger easterly opposing synoptic wind, affecting the high O3 occurrence in the western coastal area only. However, pattern 3, accounting for 21% of O3 concentration distributions, showed significantly higher O3 concentrations over the whole GSA mainly due to the retarded and slow-moving sea-breeze front under the weak opposing synoptic flow. Modeling study also indicated that local and synoptic meteorological processes play a major role in determining the high O3 concentration distribution patterns over the GSA.

  14. A scale model wind tunnel study of dispersion in the Cleveland area. Laboratory simulation of lake breeze effects on diffusion from ground level emissions

    Science.gov (United States)

    Hoydysh, W. G.

    1974-01-01

    A wind tunnel simulation of the diffusion patterns in a sea breeze was attempted. The results indicate that the low level onshore flow was well simulated for neutral, stable, unstable, and elevated inversion conditions. Velocity, turbulence, shear stress, and temperature data were taken, and the spread of emissions from ground level sources was investigated. Comparison is made with theoretical predictions by E. Inoue and with the open, homogeneous plane field results of Pasquill. Agreement with the predictions by Inoue is good, and the comparison with Pasquill's results shows that the wind tunnel flows are shifted two categories towards more stable. The discrepancy may be explained as a matter of averaging time.

  15. Regulating transcription traffic around DSBs.

    Science.gov (United States)

    Plosky, Brian S

    2015-05-07

    If a double-strand break (DSB) occurs and either a DNA polymerase or RNA polymerase is coming along, how do we save the train? In this issue of Molecular Cell, Ui et al. (2015) describe a connection between an elongation factor and a repressive complex to prevent transcription in proximity to a DSB.

  16. INTERSTELLAR NEUTRAL HELIUM IN THE HELIOSPHERE FROM IBEX OBSERVATIONS. IV. FLOW VECTOR, MACH NUMBER, AND ABUNDANCE OF THE WARM BREEZE

    Energy Technology Data Exchange (ETDEWEB)

    Kubiak, Marzena A.; Swaczyna, P.; Bzowski, M.; Sokół, J. M. [Space Research Centre of the Polish Academy of Sciences (CBK PAN), 00-716 Warsaw (Poland); Fuselier, S. A.; McComas, D. J. [Southwest Research Institute, San Antonio, TX (United States); Galli, A.; Wurz, P. [Physikalisches Institut, Universität Bern, Bern (Switzerland); Heirtzler, D.; Kucharek, H.; Leonard, T. W.; Möbius, E.; Park, J.; Schwadron, N. A., E-mail: mkubiak@cbk.waw.pl [Space Science Center and Department of Physics, University of New Hampshire, Durham, NH (United States)

    2016-04-15

    Following the high-precision determination of the velocity vector and temperature of the pristine interstellar neutral (ISN) He via a coordinated analysis summarized by McComas et al., we analyzed the Interstellar Boundary Explorer (IBEX) observations of neutral He left out from this analysis. These observations were collected during the ISN observation seasons 2010–2014 and cover the region in the Earth's orbit where the Warm Breeze (WB) persists. We used the same simulation model and a parameter fitting method very similar to that used for the analysis of ISN He. We approximated the parent population of the WB in front of the heliosphere with a homogeneous Maxwell–Boltzmann distribution function and found a temperature of ∼9500 K, an inflow speed of 11.3 km s{sup −1}, and an inflow longitude and latitude in the J2000 ecliptic coordinates 251.°6, 12.°0. The abundance of the WB relative to ISN He is 5.7% and the Mach number is 1.97. The newly determined inflow direction of the WB, the inflow directions of ISN H and ISN He, and the direction to the center of the IBEX Ribbon are almost perfectly co-planar, and this plane coincides within relatively narrow statistical uncertainties with the plane fitted only to the inflow directions of ISN He, ISN H, and the WB. This co-planarity lends support to the hypothesis that the WB is the secondary population of ISN He and that the center of the Ribbon coincides with the direction of the local interstellar magnetic field (ISMF). The common plane for the direction of the inflow of ISN gas, ISN H, the WB, and the local ISMF is given by the normal direction: ecliptic longitude 349.°7 ± 0.°6 and latitude 35.°7 ± 0.6 in the J2000 coordinates, with a correlation coefficient of 0.85.

  17. Modelling the chemistry and transport of bromoform within a sea breeze driven convective system during the SHIVA Campaign

    Science.gov (United States)

    Hamer, P. D.; Marécal, V.; Hossaini, R.; Pirre, M.; Warwick, N.; Chipperfield, M.; Samah, A. A.; Harris, N.; Robinson, A.; Quack, B.; Engel, A.; Krüger, K.; Atlas, E.; Subramaniam, K.; Oram, D.; Leedham, E.; Mills, G.; Pfeilsticker, K.; Sala, S.; Keber, T.; Bönisch, H.; Peng, L. K.; Nadzir, M. S. M.; Lim, P. T.; Mujahid, A.; Anton, A.; Schlager, H.; Catoire, V.; Krysztofiak, G.; Fühlbrügge, S.; Dorf, M.; Sturges, W. T.

    2013-08-01

    We carry out a case study of the transport and chemistry of bromoform and its product gases (PGs) in a sea breeze driven convective episode on 19 November 2011 along the North West coast of Borneo during the "Stratospheric ozone: Halogen Impacts in a Varying Atmosphere" (SHIVA) campaign. We use ground based, ship, aircraft and balloon sonde observations made during the campaign, and a 3-D regional online transport and chemistry model capable of resolving clouds and convection explicitly that includes detailed bromine chemistry. The model simulates the temperature, wind speed, wind direction fairly well for the most part, and adequately captures the convection location, timing, and intensity. The simulated transport of bromoform from the boundary layer up to 12 km compares well to aircraft observations to support our conclusions. The model makes several predictions regarding bromine transport from the boundary layer to the level of convective detrainment (11 to 12 km). First, the majority of bromine undergoes this transport as bromoform. Second, insoluble organic bromine carbonyl species are transported to between 11 and 12 km, but only form a small proportion of the transported bromine. Third, soluble bromine species, which include bromine organic peroxides, hydrobromic acid (HBr), and hypobromous acid (HOBr), are washed out efficiently within the core of the convective column. Fourth, insoluble inorganic bromine species (principally Br2) are not washed out of the convective column, but are also not transported to the altitude of detrainment in large quantities. We expect that Br2 will make a larger relative contribution to the total vertical transport of bromine atoms in scenarios with higher CHBr3 mixing ratios in the boundary layer, which have been observed in other regions. Finally, given the highly detailed description of the chemistry, transport and washout of bromine compounds within our simulations, we make a series of recommendations about the physical and

  18. Modelling the chemistry and transport of bromoform within a sea breeze driven convective system during the SHIVA Campaign

    Directory of Open Access Journals (Sweden)

    P. D. Hamer

    2013-08-01

    Full Text Available We carry out a case study of the transport and chemistry of bromoform and its product gases (PGs in a sea breeze driven convective episode on 19 November 2011 along the North West coast of Borneo during the "Stratospheric ozone: Halogen Impacts in a Varying Atmosphere" (SHIVA campaign. We use ground based, ship, aircraft and balloon sonde observations made during the campaign, and a 3-D regional online transport and chemistry model capable of resolving clouds and convection explicitly that includes detailed bromine chemistry. The model simulates the temperature, wind speed, wind direction fairly well for the most part, and adequately captures the convection location, timing, and intensity. The simulated transport of bromoform from the boundary layer up to 12 km compares well to aircraft observations to support our conclusions. The model makes several predictions regarding bromine transport from the boundary layer to the level of convective detrainment (11 to 12 km. First, the majority of bromine undergoes this transport as bromoform. Second, insoluble organic bromine carbonyl species are transported to between 11 and 12 km, but only form a small proportion of the transported bromine. Third, soluble bromine species, which include bromine organic peroxides, hydrobromic acid (HBr, and hypobromous acid (HOBr, are washed out efficiently within the core of the convective column. Fourth, insoluble inorganic bromine species (principally Br2 are not washed out of the convective column, but are also not transported to the altitude of detrainment in large quantities. We expect that Br2 will make a larger relative contribution to the total vertical transport of bromine atoms in scenarios with higher CHBr3 mixing ratios in the boundary layer, which have been observed in other regions. Finally, given the highly detailed description of the chemistry, transport and washout of bromine compounds within our simulations, we make a series of recommendations about

  19. Atmospheric boundary layer in a mountain valley during katabatic wind and valley breeze. Atmosphaerische Grenzschicht in einem Gebirgstal bei Berg- und Talwind

    Energy Technology Data Exchange (ETDEWEB)

    Freytag, C.

    1988-06-01

    This work is an attempt to investigate physical relationships during katabatic wind and valley breeze in a large Alpine valley. Being experimental in character, the work is oriented to measuring results. From measurements along the axis of the valley and in the adjacent medium-range mountains, as they were carried out during the MERKUR experiment, the budgets of mass, energy, and impulse for individual areas of the valley atmosphere could be calculated. These budgets serve as basic data for investigating the circulation, energetics, and dynamics of the mountain wind system. The introductory paragraphs give a brief overview on the state of research and open questions. The description of the experiment and of the courses of katabatic wind and valley breeze during the case study make up the second part of the work. The main part comprises the setting down of budget equations and the demonstration of the time curve and spatial structure of the individual terms. The questions raised are then discussed in the light of the author's measurements and other authors' results. Conclusions from the budget analyses are completed by simple model calculations. (orig.).

  20. Wind: backing the breeze

    Energy Technology Data Exchange (ETDEWEB)

    Wood, Elisa

    2005-11-15

    Wind development in the US rises when the 1.9 cents/kWh production tax credit (PTC) is in force and drops when it languishes. In 2005 Congress renewed the subsidy before it expired, giving the industry more than two years of assurance that the credit will be in force. The American Wind Energy Association predicts that the United States will have seen 2500 MW of new wind capacity installed during 2005, a record-breaker with almost 50% more development than the previous record year, 2001. Beyond that, the trade group expects strong and steady growth of 2000 MW during 2006 and 2007, the year the credit once again ends. Emerging Energy Research, an independent firm that analyses wind markets, technologies and strategies, forecasts that wind development could reach 3000 MW in 2006, and rise even higher in 2007. 2004 saw a meagre 389 MW of new wind projects caused by the expiry of PTC. The author reviews the demand for wind energy in the US, offshore wind potential - estimated at 900,000 MW, and emerging companies. (UK)

  1. Analyses on characteristics of sea-land breeze over Donghai island of Zhanjiang in February of 2011%湛江东海岛二月海陆风环流特征研究

    Institute of Scientific and Technical Information of China (English)

    徐峰; 王晶; 张羽; 张书文; 黄克鑫

    2012-01-01

    The average wind characteristics and sea-land breeze characteristics are analyzed by using the data of wind profiler during February 2011 on Donghai island of Zhanjiang. The results show that: the prevailing wind direction is northeasterly on Donghai island during February 2011 at an altitude of 150 m and the total frequency of occurrences of three directions E, ENE, NE is 66. 6% , in contrast, the total frequency of occurrences of westerly is only 1 % . There is an obvious difference of occurrence frequency between westerly winds and easterly winds. The hourly changes of average monthly wind speed is restricted to small range about 1 m/s from 1:00 to 15:00; and fluctuation becomes larger during 15:00 to 20:00, the maximal speed of 2. 1 m/s appears at 16:00. In February, only two days meet the conditions of sea-land hreeze. In sea-!and breeze day, the common duration of sea breeze is from 13:00 to 20:00, lasting 7 h, and land breeze's common duration is from 2:00 to 7:00, lasting 5 h. The mean speed of land breeze is 0. 8 m/s, which are smaller than 2. 1 m/s of sea breeze. The vertical height of land breezeis about 1200 m during the sea-land breeze day, which differs slightly from sea breeze. The wind speeds of both sea breeze ami land breeze have the similar (rends of increasing first and then decreasing with increasing altitude. The maximal wind speed of sea breeze appears at 750 m, and that of land breeze appears at 500 m. The sea breeze circulation is much stronger than land breeze in the region of 500 to 750 m height. The wind direction basically remains unchanged above 2 km and that is the large-scale uniform systematic west wind circulation.%利用2011年2月湛江东海岛风廓线雷达资料,系统分析了湛江东海岛2月平均风场特征及海陆风特征,结果表明:2月湛江东海岛150 m高度处以东偏北出现频率最大,在E、ENE和NE三个方位的风向出现频率之和为66.6%,偏西七个方位的风向出现频率之和仅为1

  2. A Discriminative Model for Polyphonic Piano Transcription

    Directory of Open Access Journals (Sweden)

    Poliner Graham E

    2007-01-01

    Full Text Available We present a discriminative model for polyphonic piano transcription. Support vector machines trained on spectral features are used to classify frame-level note instances. The classifier outputs are temporally constrained via hidden Markov models, and the proposed system is used to transcribe both synthesized and real piano recordings. A frame-level transcription accuracy of 68% was achieved on a newly generated test set, and direct comparisons to previous approaches are provided.

  3. Florida Agriculture - Utilizing TRMM to Analyze Sea Breeze Thunderstorm Patterns During El Nino Southern Oscillations and Their Effects Upon Available Fresh Water for South Florida Agricultural Planning and Management

    Science.gov (United States)

    Billiot, Amanda; Lee, Lucas; McKee, Jake; Cooley, Zachary Clayton; Mitchell, Brandie

    2010-01-01

    This project utilizes Tropical Rainfall Measuring Mission (TRMM) and Landsat satellite data to assess the impact of sea breeze precipitation upon areas of agricultural land use in southern Florida. Water is a critical resource to agriculture, and the availability of water for agricultural use in Florida continues to remain a key issue. Recent projections of statewide water use by 2020 estimate that 9.3 billion gallons of water per day will be demanded, and agriculture represents 47% of this demand (Bronson 2003). Farmers have fewer options for water supplies than public users and are often limited to using available supplies from surface and ground water sources which depend in part upon variable weather patterns. Sea breeze thunderstorms are responsible for much of the rainfall delivered to Florida during the wet season (May-October) and have been recognized as an important overall contributor of rainfall in southern Florida (Almeida 2003). TRMM satellite data was used to analyze how sea breeze-induced thunderstorms during El Nino and La Nina affected interannual patterns of precipitation in southern Florida from 1998-2009. TRMM's Precipitation Radar and Microwave Imager provide data to quantify water vapor in the atmosphere, precipitation rates and intensity, and the distribution of precipitation. Rainfall accumulation data derived from TRMM and other microwave sensors were used to analyze the temporal and spatial variations of rainfall during each phase of the El Nino Southern Oscillation (ENSO). Through the use of TRMM and Landsat, slight variations were observed, but it was determined that neither sea breeze nor total rainfall patterns in South Florida were strongly affected by ENSO during the study period. However, more research is needed to characterize the influence of ENSO on summer weather patterns in South Florida. This research will provide the basis for continued observations and study with the Global Precipitation Measurement Mission.

  4. Boosting transcription by transcription: enhancer-associated transcripts.

    Science.gov (United States)

    Darrow, Emily M; Chadwick, Brian P

    2013-12-01

    Enhancers are traditionally viewed as DNA sequences located some distance from a promoter that act in cis and in an orientation-independent fashion to increase utilization of specific promoters and thereby regulate gene expression. Much progress has been made over the last decade toward understanding how these distant elements interact with target promoters, but how transcription is enhanced remains an object of active inquiry. Recent reports convey the prevalence and diversity of enhancer transcription and transcripts and support both as key factors with mechanistically distinct, but not mutually exclusive roles in enhancer function. Decoupling the causes and effects of transcription on the local chromatin landscape and understanding the role of enhancer transcripts in the context of long-range interactions are challenges that require additional attention. In this review, we focus on the possible functions of enhancer transcription by highlighting several recent enhancer RNA papers and, within the context of other enhancer studies, speculate on the role of enhancer transcription in regulating differential gene expression.

  5. Language Training: English Training

    CERN Multimedia

    2004-01-01

    If you wish to participate in one of the following courses, please discuss with your supervisor and apply electronically directly from the course description pages that can be found on the Web at: http://www.cern.ch/Training/ or fill in an "application for training" form available from your Divisional Secretariat or from your DTO (Divisional Training Officer). Applications will be accepted in the order of their receipt. Language Training Françoise Benz tel. 73127 language.training@cern.ch General and Professional English Courses The next session will take place: from 1st March to 25 June 2004 (2 weeks break at Easter). These courses are open to all persons working on the Cern site, and to their spouses. For registration and further information on the courses, please consult our Web pages: http://cern.ch/Training or contact Mr. Liptow: tel. 72957.

  6. Language Training: English Training

    CERN Multimedia

    2004-01-01

    If you wish to participate in one of the following courses, please discuss with your supervisor and apply electronically directly from the course description pages that can be found on the Web at: http://www.cern.ch/Training/ or fill in an "application for training" form available from your Divisional Secretariat or from your DTO (Divisional Training Officer). Applications will be accepted in the order of their receipt. LANGUAGE TRAINING Françoise Benz tel. 73127 language.training@cern.ch General and Professional English Courses The next session will take place: from 1st March to 25 June 2004 (2 weeks break at Easter). These courses are open to all persons working on the Cern site, and to their spouses. For registration and further information on the courses, please consult our Web pages: http://cern.ch/Training or contact Mr. Liptow: tel. 72957.

  7. Research on the Interaction of Tianjin Urban Heat Island Circulation and Sea Breeze Circulation%天津地区城市热岛环流与海风环流相互作用的研究

    Institute of Scientific and Technical Information of China (English)

    东高红; 尉英华; 解以扬; 刘一玮; 李英华

    2015-01-01

    Using the mesoscale TJ-WRF model data and encryption automatic weather station (AWS)data, etc.,we analyze the distribution characteristics of Tianjin urban heat island circulation and sea breeze cir-culation as well as the interaction of them.Through several typical weather cases (Local strong thunder-storms seen in urban areas and the weather without any processes affected by the sea breeze),the trigger mechanism of the interaction between urban heat island circulation and sea breeze circulation for local thun-derstorms is analyzed.The results show that the extended height of urban heat island circulation is near 800 hPa,spatial extention is about 20 km,and the upward motion is obvious.The extended height of sea breeze circulation is near 800-750 hPa and spatial extention is about 40-60 km.The stretch height of the sea breeze front is about 950-900 hPa in the forefront of the sea breeze circulation,and the upward speed is 0.2 m·s-1 on average,slightly stronger than the heat island circulation.The sea breeze can weaken the urban heat island effect while the urban heat island can block the sea breeze circulation.With the sea breeze circulation pushed near to the urban,the sea breeze circulation and the urban heat island circulation encounter,then the two circulations get superimposed and the upward motion is significantly strength-ened,causing the maximum upward speed to go up to 0.6 m·s-1 .Under the favorable weather conditions the local severe thunderstorms can be triggered.%利用中尺度数值模式(TJ-WRF)模拟资料及天津加密自动气象站资料等,选取几个典型天气过程(城区附近局地强雷暴天气过程、受海风影响明显的无天气过程)个例,分析研究天津城市热岛环流与海风环流的空间结构特征及相互作用;重点对城市热岛环流与海风环流相互作用触发局地强雷暴的机制进行分析。结果表明:城市热岛环流的伸展高度基本在800 hPa

  8. Language Training: French Training

    CERN Multimedia

    Françoise Benz

    2004-01-01

    General and Professional French Courses The next session will take place from 11 October to 17 December 2004. These courses are open to all persons working on the CERN site, and to their spouses. For registration and further information on the courses, please consult our Web pages: http://cern.ch/Training or contact Mrs. Fontbonne: Tel. 72844. Writing Professional Documents in French This course is designed for people with a good level of spoken French. Duration: 30 hours Price: 660 CHF (for 8 students) For further information and registration, please consult our Web pages: http://cern.ch/Training or contact Mrs. Fontbonne: tel. 72844. FORMATION EN LANGUES LANGUAGE TRAINING Françoise Benz 73127 language.training@cern.ch

  9. Language Training: French Training

    CERN Document Server

    Françoise Benz

    2005-01-01

    General and Professional French Courses The next session will take place from 18 April to 30 June 2005. These courses are open to all persons working on the CERN site, and to their spouses. For registration and further information on the courses, please consult our Web pages: http://cern.ch/Training or contact Mrs. Benz : Tel. 73127. Writing Professional Documents in French The next session will take place from 18 April to 30 June 2005. This course is designed for people with a good level of spoken French. Duration: 30 hours Price: 660 CHF (for 8 students) For further information and registration, please consult our Web pages: http://cern.ch/Training or contact Mrs. Benz : Tel. 73127. FORMATION EN LANGUES LANGUAGE TRAINING Françoise Benz 73127 language.training@cern.ch

  10. Language Training: French Training

    CERN Multimedia

    2004-01-01

    If you wish to participate in one of the following courses, please discuss with your supervisor and apply electronically directly from the course description pages that can be found on the Web at: http://www.cern.ch/Training/ or fill in an "application for training" form available from your Divisional Secretariat or from your DTO (Divisional Training Officer). Applications will be accepted in the order of their receipt. LANGUAGE TRAINING Françoise Benz tel. 73127 language.training@cern.ch General and Professional French Courses The next session will take place from 26 April to 02 July 2004. These courses are open to all persons working on the Cern site, and to their spouses. For registration and further information on the courses, please consult our Web pages: http://cern.ch/Training or contact Mrs. Benz: Tel. 73127. Writing Professional Documents in French The next session will take place from 26 April to 02 July 2004. This course is designed for people with a good level...

  11. Language Training: French Training

    CERN Document Server

    2004-01-01

    If you wish to participate in one of the following courses, please discuss with your supervisor and apply electronically directly from the course description pages that can be found on the Web at: http://www.cern.ch/Training/ or fill in an "application for training" form available from your Divisional Secretariat or from your DTO (Divisional Training Officer). Applications will be accepted in the order of their receipt. LANGUAGE TRAINING Françoise Benz tel. 73127 language.training@cern.ch General and Professional French Courses The next session will take place from 26 April to 02 July 2004. These courses are open to all persons working on the Cern site, and to their spouses. For registration and further information on the courses, please consult our Web pages: http://cern.ch/Training or contact Mrs. Benz: Tel. 73127. Writing Professional Documents in French The next session will take place from 26 April to 02 July 2004. This course is designed for people with a good level of s...

  12. Harvesting wind energy from the sea breeze in peri-urban coastal areas by means of small scale wind turbines - Case study: Viladecans, Llobregat Delta, northeast of Iberian Peninsula

    Science.gov (United States)

    Rojas, Jose I.; Cabrera, Barbara; Mazon, Jordi

    2016-04-01

    Wind speed data recorded during 18 years (1993-2010) in the Llobregat Delta (15 km south of Barcelona city; northeast of the Iberian Peninsula) were used to assess the wind energy generated by off-grid small scale wind turbines (the IT-PE-100 and the HP-600W) for the whole year and for the sea breeze period. The computations were made using QBlade, FAST and AeroDyn simulation tools and manufacturer power curves. Using manufacturer data, the HP-600W with hub-height 8 m would deliver 157 kWh during the whole year (78 kWh during the sea breeze period), with an average power of 18 W (37 W). In this work, the results of the simulations are compared with power and energy production data measured in an HP-600W turbine installed in situ from December 2014 to April 2016. Also, the measured power is compared to the power obtained by applying the measured wind in the period 2014-2016 to the manufacturer power curve and the power curve obtained with the simulations. The results of the computations agree with the experimental data, thus validating the proposed approach for wind resource estimation. The feasibility of using a vertical axis wind turbine for obtaining wind energy from the local, thermal wind regimes is also studied. This research confirms that the sea-breeze is an interesting wind energy resource for micro-generation in peri-urban coastal areas where large-scale wind farms cannot be implemented.

  13. Training safely, Training safety

    Directory of Open Access Journals (Sweden)

    Jianjun Wu

    2014-09-01

    Full Text Available It is the basic requirement of maritime safety education to guarantee the safety of teaching operation while training the crew's occupation safety capability. Marine Training Center of Shanghai Maritime University has undertaken the practical teaching of "marine survival" for many years and come up with the whole safety procedures of training. Based on the requirements of SOLAS convention and regulations of STCW over crew training, this paper introduces the safety allocation, utilization and maintenance of teaching equipments. Through the investigation of the safety situation of students' practical operation, the safety teaching method named "four in one" has been put forward, which includes the pre-teaching safety precaution, the whole monitor during the teaching process, the post-teaching summary evaluation, and the reset and standby of teaching facilities. Finally, during the learning and training of "marine survival", crews and students are called on to place priority on personal safety rather than acquisition of knowledge and skills. Only in this way can they be capable of self-protection and protection of others in the career of seafaring.

  14. Mapping Yeast Transcriptional Networks

    OpenAIRE

    Hughes, Timothy R; de Boer, Carl G.

    2013-01-01

    The term “transcriptional network” refers to the mechanism(s) that underlies coordinated expression of genes, typically involving transcription factors (TFs) binding to the promoters of multiple genes, and individual genes controlled by multiple TFs. A multitude of studies in the last two decades have aimed to map and characterize transcriptional networks in the yeast Saccharomyces cerevisiae. We review the methodologies and accomplishments of these studies, as well as challenges we now face....

  15. Massively Systematic Transcript End Readout (MASTER): Transcription Start Site Selection, Transcriptional Slippage, and Transcript Yields

    Science.gov (United States)

    Vvedenskaya, Irina O.; Zhang, Yuanchao; Goldman, Seth R.; Valenti, Anna; Visone, Valeria; Taylor, Deanne M.; Ebright, Richard H.; Nickels, Bryce E.

    2015-01-01

    SUMMARY We report the development of a next-generation sequencing-based technology that entails construction of a DNA library comprising up to at least 47 (~16,000) bar-coded sequences, production of RNA transcripts, and analysis of transcript ends and transcript yields ("massively systematic transcript end readout," MASTER). Using MASTER, we define full inventories of transcription start sites ("TSSomes") of Escherichia coli RNA polymerase for initiation at a consensus core promoter in vitro and in vivo, we define the TSS-region DNA-sequence determinants for TSS selection, reiterative initiation ("slippage synthesis"), and transcript yield, and we define effects of DNA topology and NTP concentration. The results reveal that slippage synthesis occurs from the majority of TSS-region DNA sequences and that TSS-region DNA sequences have profound, up to 100-fold, effects on transcript yield. The results further reveal that TSSomes depend on DNA topology, consistent with the proposal that TSS selection involves transcription-bubble expansion ("scrunching") and transcription-bubble contraction ("anti-scrunching"). PMID:26626484

  16. Massively Systematic Transcript End Readout, "MASTER": Transcription Start Site Selection, Transcriptional Slippage, and Transcript Yields.

    Science.gov (United States)

    Vvedenskaya, Irina O; Zhang, Yuanchao; Goldman, Seth R; Valenti, Anna; Visone, Valeria; Taylor, Deanne M; Ebright, Richard H; Nickels, Bryce E

    2015-12-17

    We report the development of a next-generation sequencing-based technology that entails construction of a DNA library comprising up to at least 4(7) (∼ 16,000) barcoded sequences, production of RNA transcripts, and analysis of transcript ends and transcript yields (massively systematic transcript end readout, "MASTER"). Using MASTER, we define full inventories of transcription start sites ("TSSomes") of Escherichia coli RNA polymerase for initiation at a consensus core promoter in vitro and in vivo; we define the TSS-region DNA sequence determinants for TSS selection, reiterative initiation ("slippage synthesis"), and transcript yield; and we define effects of DNA topology and NTP concentration. The results reveal that slippage synthesis occurs from the majority of TSS-region DNA sequences and that TSS-region DNA sequences have profound, up to 100-fold, effects on transcript yield. The results further reveal that TSSomes depend on DNA topology, consistent with the proposal that TSS selection involves transcription-bubble expansion ("scrunching") and transcription-bubble contraction ("anti-scrunching").

  17. A Nonnatural Transcriptional Coactivator

    Science.gov (United States)

    Nyanguile, Origene; Uesugi, Motonari; Austin, David J.; Verdine, Gregory L.

    1997-12-01

    In eukaryotes, sequence-specific DNA-binding proteins activate gene expression by recruiting the transcriptional apparatus and chromatin remodeling proteins to the promoter through protein-protein contacts. In many instances, the connection between DNA-binding proteins and the transcriptional apparatus is established through the intermediacy of adapter proteins known as coactivators. Here we describe synthetic molecules with low molecular weight that act as transcriptional coactivators. We demonstrate that a completely nonnatural activation domain in one such molecule is capable of stimulating transcription in vitro and in vivo. The present strategy provides a means of gaining external control over gene activation through intervention using small molecules.

  18. Evaluating Training

    Institute of Scientific and Technical Information of China (English)

    Zhang Huanhai; Zhang Lei's

    2008-01-01

    @@ Clause 6.2.2: Competence, Awareness and Training in ISO9001:2000 standard sets the training requirements for organizations. Problems with evaluating the outcomes of training arise during examination and verification.

  19. Language Training: English Training

    CERN Document Server

    Françoise Benz

    2004-01-01

    If you wish to participate in one of the following courses, please discuss with your supervisor and apply electronically directly from the course description pages that can be found on the Web at: http://www.cern.ch/Training/ or fill in an 'application for training' form available from your Divisional Secretariat or from your DTO (Divisional Training Officer). Applications will be accepted in the order of their receipt. General and Professional English Courses The next session will take place from 04 October 2004 to 11 February 2005 (3 weeks break at Christmas). These courses are open to all persons working on the CERN site, and to their spouses. For registration and further information on the courses, please consult our Web pages: http://cern.ch/Training or contact Mr. Liptow, tel. 72957. Oral Expression This course is intended for people with a good knowledge of English who want to practise and maintain their speaking skills while extending their vocabulary. There will be approximately 8 participants in...

  20. The Transcription Factor Encyclopedia

    DEFF Research Database (Denmark)

    Yusuf, Dimas; Butland, Stefanie L; Swanson, Magdalena I

    2012-01-01

    ABSTRACT: Here we present the Transcription Factor Encyclopedia (TFe), a new web-based compendium of mini review articles on transcription factors (TFs) that is founded on the principles of open access and collaboration. Our consortium of over 100 researchers has collectively contributed over 130...

  1. The transcriptional landscape

    DEFF Research Database (Denmark)

    Nielsen, Henrik

    2011-01-01

    The application of new and less biased methods to study the transcriptional output from genomes, such as tiling arrays and deep sequencing, has revealed that most of the genome is transcribed and that there is substantial overlap of transcripts derived from the two strands of DNA. In protein codi...

  2. 海风锋在渤海西岸局地暴雨过程中的作用%The Role of Sea Breeze Front in Local Storm of Bohai Coast

    Institute of Scientific and Technical Information of China (English)

    东高红; 何群英; 刘一玮; 解以扬; 戴云伟

    2011-01-01

    利用常规观测资料、地面加密自动站资料、多普勒雷达观测资料及中尺度TJ-WRF模式输出资料,对2009年7月6日天津宁河地区出现的暴雨天气过程进行了分析,重点分析了渤海湾海风锋对沿岸局地暴雨的触发机理。结果表明:此次局地暴雨过程是在有利天气背景条件下发生的,暴雨发生地存在局地层结不稳定和较好的水汽条件。海风锋本身有一辐合抬升区,区域内有弱对流存在;当海风锋移到局地存在层结不稳定且水汽充足的区域,其抬升区的辐合上升运动迅速加强,从而触发该地区雷暴的新生发展;海风锋与迎面移来的雷暴相遇,会对雷暴的加速发展起到加强作用。利用中尺度WRF模式输出资料进行分析看到,两条辐合线相交处易激发出强雷暴,雷暴出现在近地层大气暖干区的北端、湿空气的交汇处;海风锋对雷暴的新生发展有明显的触发抬升作用。%Using the data obtained by conventional observations,ground densified automatic weather stations, Doppler radar observations and mesoscale TJ-WRF model,we analyze the heavy rain occurring on 6 July 2009 at Ninghe District of Tianjin and highlight the trigger mechanism of coastal local storm caused by sea breeze front of the Bohai Gulf.The results show that the heavy rain occurred in the favorable weather conditions,and there were local instability and adequate vapor conditions.Sea breeze front itself has a convergent uplift area where weak convection exists;when sea breeze front moves to the unstable and adequate vapor areas,it will strengthen the convergence positive movement of convergent uplift area and lead to the new storm development.The meeting of the sea breeze front and thunderstorm will play a strengthened role in its development.By analyzing the data obtained by mesoscale WRF model,we can see that at the warm and dry ground in front area near the north end of the wet air interchange

  3. PROSPECTS FOR COKE BREEZE RECOVERY

    Directory of Open Access Journals (Sweden)

    D. N. Mihnovets

    2012-01-01

    Full Text Available Researches give grounds to believe in the possibility of receiving briquettes from coke waste mixed with peat dry coal and their use for smelting iron in the cupola or as a household fuel.

  4. DNA supercoiling during transcription

    Science.gov (United States)

    Ma, Jie; Wang, Michelle D.

    2017-01-01

    The twin-supercoiled-domain model describes how transcription can drive DNA supercoiling, and how DNA supercoiling, in turn plays an important role in regulating gene transcription. In vivo and in vitro experiments have disclosed many details of the complex interactions in this relationship, and recently new insights have been gained with the help of genome-wide DNA supercoiling mapping techniques and single molecule methods. This review summarizes the general mechanisms of the interplay between DNA supercoiling and transcription, considers the biological implications, and focuses on recent important discoveries and technical advances in this field. We highlight the significant impact of DNA supercoiling in transcription, but also more broadly in all processes operating on DNA.

  5. Modeling of Slovak Language for Broadcast News Transcription

    OpenAIRE

    Staš, Ján; JUHÁR Jozef

    2015-01-01

    The paper describes recent progress in the development the Slovak language models for transcription of spontaneous speech such as broadcast news, educational talks and lectures, or meetings. This work extends previous research oriented on the automatic transcription of dictated speech and brings some new extensions for improving perplexity and robustness of the Slovak language models trained on the web-based and electronic language resources for being more precise in recognition of spontaneou...

  6. 架空输电线路微风振动防治措施介绍及瞻望%Measures of Introduction and Look Forward to Prevent Breeze Vibration of Overhead Power Transmission Lines

    Institute of Scientific and Technical Information of China (English)

    李阔; 成印健; 成志

    2013-01-01

    随着输电线路大量兴建,输电线路微风振动问题也越来越引起线路工作人员的重视。为了防止线路因微风振动引起的破坏,在线路设计时要加强措施来防止振动破坏。采用自阻尼导线和防振线夹来防振是一种通过改变线路自身组成部分特性来防振的措施,采用护线条、阻尼线、防振锤、阻尼弹簧间隔棒这些消振装置来防振是另外一种有效的防振措施,两种方法可根据具体情况来选择采用。同时,在超高压乃至特高压迅速发展的今天,利用阻尼线与防振锤混合消振装置以及阻尼弹簧间隔棒来防止微风振动已经越来越受到电力工作者的青睐,成为了一种趋势。%Along with the power transmission lines of construction ,the problem to prevent breeze vibration has caused line workers' atten-tion more and more. To prevent lines for vibration caused by the breeze destruction,in the line design people must strengthen the measures to pre-vent the vibration damage. Adopting the damping wires and vibration- proof line clip to prevent vibration is an effective way by which changing the line itself component characteristics. Adopting Armor Rod, Damping Line, Vibration- proof Hammer,damping spring spacer these vibrating de-vices to prevent vibration is another kind of effective vibration control measures . The two methods can be adopted according to the specific condi-tions. At the same time when the extra high voltage system and even ultra high voltage system is on the development, Using damping line and vi-bration- proof hammer mixed vibrating device class and damping spring spacer to prevent breeze vibration has got the favour of power workers more and more.

  7. Language Training: English Training

    CERN Multimedia

    Françoise Benz

    2004-01-01

    General and Professional English Courses The next session will take place from 04 October 2004 to 11 February 2005 (3 weeks break at Christmas). These courses are open to all persons working on the CERN site, and to their spouses. For registration and further information on the courses, please consult our Web pages: http://cern.ch/Training or contact Mr. Liptow, tel. 72957. Oral Expression This course is intended for people with a good knowledge of English who want to practise and maintain their speaking skills while extending their vocabulary. There will be approximately 8 participants in a class. Speaking activities will include discussions, meeting simulations, role-plays etc. depending on the needs of the students. Duration: 20 hours (2 hours a week) Price: 440 CHF (for 8 students) For further information, please contact Mr. Liptow, tel. 72957. Date and timetable will be fixed when there are sufficient participants enrolled. FORMATION EN LANGUES LANGUAGE TRAINING Françoise Benz 73127 langua...

  8. Language Training: English Training

    CERN Document Server

    Françoise Benz

    2004-01-01

    La prochaine session se déroulera du 04 octobre 2004 au 11 février 2005 (interruption de 3 semaines à Noël). Ces cours s'adressent à toute personne travaillant au CERN ainsi qu'à leur conjoint. Pour vous inscrire et voir tout le détail des cours proposés, consultez nos pages Web : http://cern.ch/Training Vous pouvez aussi contacter M. Liptow, tél. 72957. General and Professional English Courses The next session will take place from 04 October 2004 to 11 February 2005 (3 weeks break at Christmas). These courses are open to all persons working on the CERN site, and to their spouses. For registration and further information on the courses, please consult our Web pages: http://cern.ch/Training or contact Mr. Liptow, tel. 72957. Oral Expression This course is intended for people with a good knowledge of English who want to practise and maintain their speaking skills while extending their vocabulary. There will be approximately 8 participants ...

  9. Language Training: English Training

    CERN Multimedia

    Françoise Benz

    2004-01-01

    General and Professional English Courses The next session will take place from 04 October 2004 to 11 February 2005 (3 weeks break at Christmas). These courses are open to all persons working on the CERN site, and to their spouses. For registration and further information on the courses, please consult our Web pages: http://cern.ch/Training or contact Mr. Liptow, tel. 72957. Oral Expression This course is intended for people with a good knowledge of English who want to practise and maintain their speaking skills while extending their vocabulary. There will be approximately 8 participants in a class. Speaking activities will include discussions, meeting simulations, role-plays etc. depending on the needs of the students. Duration: 20 hours (2 hours a week) Price: 440 CHF (for 8 students) For further information, please contact Mr. Liptow, tel. 72957. Date and timetable will be fixed when there are sufficient participants enrolled. FORMATION EN LANGUES LANGUAGE TRAINING Françoise Benz 73127 languag...

  10. Language Training - French Training

    CERN Multimedia

    2007-01-01

    General and Professional French Courses The next session will take place from 29 January to 30 March 2007. These courses are open to all persons working on the CERN site, and to their spouses. For registration and further information on the courses, please consult our Web pages: http://cern.ch/Training or contact Mrs. Benz : Tel. 73127. Writing Professional Documents in French The next session will take place from 29 January to 30 March 2007. This course is designed for people with a good level of spoken French. Duration: 30 hours Price: 660 CHF (for 8 students) For further information and registration, please consult our Web pages:   http://cern.ch/Training or contact Mrs. Benz : Tel. 73127. Writing Professional Documents in English The next session will take place from January to June 2007 (break at Easter). This course is designed for people with a good level of spoken English. Duration: 30 hours Price: 660 CHF (for 8 students) Timetable will be fixed after discussion with the students. For registratio...

  11. Strength Training

    Science.gov (United States)

    ... strengthens your heart and lungs. When you strength train with weights, you're using your muscles to ... see there are lots of different ways to train with weights. Try a few good basic routines ...

  12. The transcription factor encyclopedia.

    Science.gov (United States)

    Yusuf, Dimas; Butland, Stefanie L; Swanson, Magdalena I; Bolotin, Eugene; Ticoll, Amy; Cheung, Warren A; Zhang, Xiao Yu Cindy; Dickman, Christopher T D; Fulton, Debra L; Lim, Jonathan S; Schnabl, Jake M; Ramos, Oscar H P; Vasseur-Cognet, Mireille; de Leeuw, Charles N; Simpson, Elizabeth M; Ryffel, Gerhart U; Lam, Eric W-F; Kist, Ralf; Wilson, Miranda S C; Marco-Ferreres, Raquel; Brosens, Jan J; Beccari, Leonardo L; Bovolenta, Paola; Benayoun, Bérénice A; Monteiro, Lara J; Schwenen, Helma D C; Grontved, Lars; Wederell, Elizabeth; Mandrup, Susanne; Veitia, Reiner A; Chakravarthy, Harini; Hoodless, Pamela A; Mancarelli, M Michela; Torbett, Bruce E; Banham, Alison H; Reddy, Sekhar P; Cullum, Rebecca L; Liedtke, Michaela; Tschan, Mario P; Vaz, Michelle; Rizzino, Angie; Zannini, Mariastella; Frietze, Seth; Farnham, Peggy J; Eijkelenboom, Astrid; Brown, Philip J; Laperrière, David; Leprince, Dominique; de Cristofaro, Tiziana; Prince, Kelly L; Putker, Marrit; del Peso, Luis; Camenisch, Gieri; Wenger, Roland H; Mikula, Michal; Rozendaal, Marieke; Mader, Sylvie; Ostrowski, Jerzy; Rhodes, Simon J; Van Rechem, Capucine; Boulay, Gaylor; Olechnowicz, Sam W Z; Breslin, Mary B; Lan, Michael S; Nanan, Kyster K; Wegner, Michael; Hou, Juan; Mullen, Rachel D; Colvin, Stephanie C; Noy, Peter John; Webb, Carol F; Witek, Matthew E; Ferrell, Scott; Daniel, Juliet M; Park, Jason; Waldman, Scott A; Peet, Daniel J; Taggart, Michael; Jayaraman, Padma-Sheela; Karrich, Julien J; Blom, Bianca; Vesuna, Farhad; O'Geen, Henriette; Sun, Yunfu; Gronostajski, Richard M; Woodcroft, Mark W; Hough, Margaret R; Chen, Edwin; Europe-Finner, G Nicholas; Karolczak-Bayatti, Magdalena; Bailey, Jarrod; Hankinson, Oliver; Raman, Venu; LeBrun, David P; Biswal, Shyam; Harvey, Christopher J; DeBruyne, Jason P; Hogenesch, John B; Hevner, Robert F; Héligon, Christophe; Luo, Xin M; Blank, Marissa Cathleen; Millen, Kathleen Joyce; Sharlin, David S; Forrest, Douglas; Dahlman-Wright, Karin; Zhao, Chunyan; Mishima, Yuriko; Sinha, Satrajit; Chakrabarti, Rumela; Portales-Casamar, Elodie; Sladek, Frances M; Bradley, Philip H; Wasserman, Wyeth W

    2012-01-01

    Here we present the Transcription Factor Encyclopedia (TFe), a new web-based compendium of mini review articles on transcription factors (TFs) that is founded on the principles of open access and collaboration. Our consortium of over 100 researchers has collectively contributed over 130 mini review articles on pertinent human, mouse and rat TFs. Notable features of the TFe website include a high-quality PDF generator and web API for programmatic data retrieval. TFe aims to rapidly educate scientists about the TFs they encounter through the delivery of succinct summaries written and vetted by experts in the field. TFe is available at http://www.cisreg.ca/tfe.

  13. Language Training - English Training

    CERN Multimedia

    HR Department

    2009-01-01

    General and Professional English Courses The next session will take place: from 2nd March to end of June 2009 (1/2 weeks break at Easter). These courses are open to all persons working on the CERN site, and to their spouses. For registration and further information on the courses, please consult our Web pages: http://cern.ch/Training or contact Nathalie Dumeaux, tel. 78144. Oral Expression The next session will take place from 2nd March to end of June 2009 (1/2 weeks break at Easter). This course is intended for people with a good knowledge of English who want to enhance their speaking skills. There will be on average of 8 participants in a class. Speaking activities will include discussions, meeting simulations, role-plays etc. depending on the needs of the students. Duration: 30 hours Price: 660 CHF (for a minimum of 8 students) Writing Professional Documents in English The next session will take place from 2nd March to end of June 2009 (1/2 weeks break at Easter). This course is designed for people w...

  14. Language Training - English Training

    CERN Multimedia

    HR Department

    2009-01-01

    General and Professional English Courses The next session will take place: from 2nd March to end of June 2009 (1/2 weeks break at Easter). These courses are open to all persons working on the CERN site, and to their spouses. For registration and further information on the courses, please consult our Web pages: http://cern.ch/Training or contact Nathalie Dumeaux, tel. 78144. Oral Expression The next session will take place from 2nd March to end of June 2009 (1/2 weeks break at Easter). This course is intended for people with a good knowledge of English who want to enhance their speaking skills. There will be an average of 8 participants per class. Speaking activities will include discussions, meeting simulations, role-plays, etc., depending on the needs of the students. Duration: 30 hours Price: 660 CHF (for a minimum of 8 students) Writing Professional Documents in English The next session will take place from 2nd March to end of June 2009 (1/2 weeks break at Easter). This course is designed for people ...

  15. Rhythm quantization for transcription

    NARCIS (Netherlands)

    Cemgil, A.T.; Desain, P.W.M.; Kappen, H.J.

    1999-01-01

    Automatic Music Transcription is the extraction of an acceptable notation from performed music. One important task in this problem is rhythm quantization which refers to categorization of note durations. Although quantization of a pure mechanical performance is rather straightforward, the task becom

  16. Mapping yeast transcriptional networks.

    Science.gov (United States)

    Hughes, Timothy R; de Boer, Carl G

    2013-09-01

    The term "transcriptional network" refers to the mechanism(s) that underlies coordinated expression of genes, typically involving transcription factors (TFs) binding to the promoters of multiple genes, and individual genes controlled by multiple TFs. A multitude of studies in the last two decades have aimed to map and characterize transcriptional networks in the yeast Saccharomyces cerevisiae. We review the methodologies and accomplishments of these studies, as well as challenges we now face. For most yeast TFs, data have been collected on their sequence preferences, in vivo promoter occupancy, and gene expression profiles in deletion mutants. These systematic studies have led to the identification of new regulators of numerous cellular functions and shed light on the overall organization of yeast gene regulation. However, many yeast TFs appear to be inactive under standard laboratory growth conditions, and many of the available data were collected using techniques that have since been improved. Perhaps as a consequence, comprehensive and accurate mapping among TF sequence preferences, promoter binding, and gene expression remains an open challenge. We propose that the time is ripe for renewed systematic efforts toward a complete mapping of yeast transcriptional regulatory mechanisms.

  17. Transcription Dynamics in Living Cells.

    Science.gov (United States)

    Lenstra, Tineke L; Rodriguez, Joseph; Chen, Huimin; Larson, Daniel R

    2016-07-01

    The transcription cycle can be roughly divided into three stages: initiation, elongation, and termination. Understanding the molecular events that regulate all these stages requires a dynamic view of the underlying processes. The development of techniques to visualize and quantify transcription in single living cells has been essential in revealing the transcription kinetics. They have revealed that (a) transcription is heterogeneous between cells and (b) transcription can be discontinuous within a cell. In this review, we discuss the progress in our quantitative understanding of transcription dynamics in living cells, focusing on all parts of the transcription cycle. We present the techniques allowing for single-cell transcription measurements, review evidence from different organisms, and discuss how these experiments have broadened our mechanistic understanding of transcription regulation.

  18. Non-transcriptional regulatory processes shape transcriptional network dynamics

    OpenAIRE

    Ray, J. Christian J; Tabor, Jeffrey J.; Igoshin, Oleg A.

    2011-01-01

    Information about the extra- or intracellular environment is often captured as biochemical signals propagating through regulatory networks. These signals eventually drive phenotypic changes, typically by altering gene expression programs in the cell. Reconstruction of transcriptional regulatory networks has given a compelling picture of bacterial physiology, but transcriptional network maps alone often fail to describe phenotypes. In many cases, the dynamical performance of transcriptional re...

  19. DNA Topoisomerases in Transcription

    DEFF Research Database (Denmark)

    Rødgaard, Morten Terpager

    2015-01-01

    This Ph.D. thesis summarizes the main results of my studies on the interplay between DNA topoisomerases and transcription. The work was performed from 2011 to 2015 at Aarhus University in the Laboratory of Genome Research, and was supervised by associate professor Anni H. Andersen. Most of the ex......This Ph.D. thesis summarizes the main results of my studies on the interplay between DNA topoisomerases and transcription. The work was performed from 2011 to 2015 at Aarhus University in the Laboratory of Genome Research, and was supervised by associate professor Anni H. Andersen. Most...... topoisomerase-DNA cleavage complex. The second study is an investigation of how topoisomerases influence gene regulation by keeping the genome in an optimal topological state....

  20. SNFing HIV transcription

    Directory of Open Access Journals (Sweden)

    Bukrinsky Michael

    2006-08-01

    Full Text Available Abstract The SWI/SNF chromatin remodeling complex is an essential regulator of transcription of cellular genes. HIV-1 infection induces exit of a core component of SWI/SNF, Ini1, into the cytoplasm and its association with the viral pre-integration complex. Several recent papers published in EMBO Journal, Journal of Biological Chemistry, and Retrovirology provide new information regarding possible functions of Ini1 and SWI/SNF in HIV life cycle. It appears that Ini1 has an inhibitory effect on pre-integration steps of HIV replication, but also contributes to stimulation of Tat-mediated transcription. This stimulation involves displacement of the nucleosome positioned at the HIV promoter.

  1. ENGLISH TRAINING

    CERN Multimedia

    2003-01-01

    If you wish to participate in one of the following courses, please discuss with your supervisor and apply electronically directly from the course description pages that can be found on the Web at: http://www.cern.ch/Training/ or fill in an "application for training" form available from your Divisional Secretariat or from your DTO (Divisional Training Officer). Applications will be accepted in the order of their receipt. LANGUAGE TRAINING Françoise Benz tel. 73127 language.training@cern.ch PLACES AVAILABLE Writing Professional Documents in English This course is designed for people with a good level of spoken English who need to improve their professional writing (administrative, scientific, technical). Duration: 20 hours (2 hours a week) Price: 440 CHF (for 8 students) Date and timetable will be fixed when there are sufficient participants enrolled. Oral Expression This course is intended for people with a good knowledge of English who want to practise and maintain their sp...

  2. Training scientists and engineers for the year 2000

    Energy Technology Data Exchange (ETDEWEB)

    Trivelpiece, A.W.

    1990-05-08

    This paper is a transcript of testimony by Alvin W. Trivelpiece, director of ORNL, before Congressional Subcommittee on Science, Technology, and Space. Dr. Trivelpiece discusses the importance of training scientist and engineers for the year 2000. (FSD)

  3. Non-transcriptional regulatory processes shape transcriptional network dynamics.

    Science.gov (United States)

    Ray, J Christian J; Tabor, Jeffrey J; Igoshin, Oleg A

    2011-10-11

    Information about the extra- or intracellular environment is often captured as biochemical signals that propagate through regulatory networks. These signals eventually drive phenotypic changes, typically by altering gene expression programmes in the cell. Reconstruction of transcriptional regulatory networks has given a compelling picture of bacterial physiology, but transcriptional network maps alone often fail to describe phenotypes. Cellular response dynamics are ultimately determined by interactions between transcriptional and non-transcriptional networks, with dramatic implications for physiology and evolution. Here, we provide an overview of non-transcriptional interactions that can affect the performance of natural and synthetic bacterial regulatory networks.

  4. Training Attestations

    CERN Multimedia

    HR Department

    2004-01-01

    In the past, paper Training Attestations were printed during the first quarter of a New Year for Staff Members having followed courses and seminars the previous year in the context of CERN's official Training Programmes.  The attestations were sent to Divisional Training Officers (DTO's) for distribution to all Staff Members concerned within their Division. In practise many Staff Members did not find this paper useful, however some expressed a wish to be able to have access to a self-service facility in case of need. This change was discussed by the Joint Training Board (JTB) and with the DTO's during 2003 and was supported. As a consequence, following a collaboration between HR-PMD and IT-AIS, from 2004 onwards paper Training Attestations will no longer be printed and distributed as before. Those requiring a list of training followed in the past can now obtain their attestation directly from the Human Resources Toolkit (HRT) application for all training followed since 1997. https://hrt.cern...

  5. LANGUAGE TRAINING

    CERN Multimedia

    2004-01-01

    If you wish to participate in one of the following courses, please discuss with your supervisor and apply electronically directly from the course description pages that can be found on the Web at: http://www.cern.ch/Training/ or fill in an "application for training" form available from your Divisional Secretariat or from your DTO (Divisional Training Officer). Applications will be accepted in the order of their receipt. LANGUAGE TRAINING Françoise Benz tel. 73127 language.training@cern.ch FRENCH TRAINING General and Professional French Courses The next session will take place from 26 January to 02 April 2004. These courses are open to all persons working on the Cern site, and to their spouses. For registration and further information on the courses, please consult our Web pages: http://cern.ch/Training or contact Mrs. Benz : Tel. 73127. Writing Professional Documents in French The next session will take place from 26 January to 02 April 2004. This course is designed for peop...

  6. LANGUAGE TRAINING

    CERN Multimedia

    2004-01-01

    If you wish to participate in one of the following courses, please discuss with your supervisor and apply electronically directly from the course description pages that can be found on the Web at: http://www.cern.ch/Training/ or fill in an "application for training" form available from your Divisional Secretariat or from your DTO (Divisional Training Officer). Applications will be accepted in the order of their receipt. LANGUAGE TRAINING Françoise Benz tel. 73127 language.training@cern.ch FRENCH TRAINING General and Professional French Courses The next session will take place from 26 January to 02 April 2004. These courses are open to all persons working on the Cern site, and to their spouses. For registration and further information on the courses, please consult our Web pages: http://cern.ch/Training or contact Mrs. Benz: Tel. 73127. Writing Professional Documents in French The next session will take place from 26 January to 02 April 2004. This course is designed for people wi...

  7. Addressing some Common Problems in Transcript Analysis

    Directory of Open Access Journals (Sweden)

    Patrick J. Fahy

    2001-01-01

    Full Text Available Computer conferencing is one of the more useful parts of computer-mediated communications (CMC, and is virtually ubiquitous in distance education. The temptation to analyze the resulting interaction has resulted in only partial success, however (Henri, 1992; Kanuka and Anderson, 1998; Rourke, Anderson, Garrison and Archer, 1999; Fahy, Crawford, Ally, Cookson, Keller and Prosser, 2000. Some suggest the problem is made more complex by failings of both technique and, more seriously, theory capable of guiding transcript analysis research (Gunawardena, Lowe and Anderson, 1997.We have previously described development and pilot-testing of an instrument and a process for transcript analysis, call the the TAT (Transcript Analysis Tool, based on a model originally developed by Zhu (1996. We found that the instrument and coding procedures used provided acceptable – sometimes excellent – levels of interrater reliability (varying from 70 percent to 94 percent in pilot applications, depending upon user training and practice with the instrument, and that results of pilots indicated the TAT discriminated well among the various types of statements found in online conferences (Fahy, et al., 2000.

  8. Deciphering Transcriptional Regulation

    DEFF Research Database (Denmark)

    Valen, Eivind

    in different cell types. This thesis presents several methods for analysis and description of promoters. We focus particularly the binding sites of TFs and computational methods for locating these. We contribute to the ¿eld by compiling a database of binding preferences for TFs which can be used for site...... published providing an unbiased overview of the transcription start site (TSS) usage in a tissue. We have paired this method with high-throughput sequencing technology to produce a library of unprecedented depth (DeepCAGE) for the mouse hippocampus. We investigated this in detail and focused particularly...

  9. Strength Training

    Science.gov (United States)

    ... en español Entrenamiento de la fuerza muscular Strength training is a vital part of a balanced exercise routine that includes aerobic activity and flexibility exercises. Regular aerobic exercise, such as running or ...

  10. Supervisor training

    DEFF Research Database (Denmark)

    Pedersen, Inge Nygaard

    2015-01-01

    This article fills a gap in knowledge about supervisor training programmes in the field of music therapy in Europe. Sparse research exists which demonstrates evidence of effective professional supervision upon the outcome of music therapy clinical practice. The article has its focus on the experi......This article fills a gap in knowledge about supervisor training programmes in the field of music therapy in Europe. Sparse research exists which demonstrates evidence of effective professional supervision upon the outcome of music therapy clinical practice. The article has its focus...... on the experience of an integrated supervisor training programme offered in Aalborg, Denmark in 2009/2010. In this programme general issues of professional supervision and the application of artistic media as a core element in the supervisory process were Integrated. It is the hope of the author that this article...... will inspire other music therapists to develop supervisor training programmes for professional music therapists and also to undertake further research into professional supervision....

  11. Nucleocytoplasmic shuttling of transcription factors

    DEFF Research Database (Denmark)

    Cartwright, P; Helin, K

    2000-01-01

    To elicit the transcriptional response following intra- or extracellular stimuli, the signals need to be transmitted to their site of action within the nucleus. The nucleocytoplasmic shuttling of transcription factors is a mechanism mediating this process. The activation and inactivation...... of the transcriptional response is essential for cells to progress through the cell cycle in a normal manner. The involvement of cytoplasmic and nuclear accessory molecules, and the general nuclear membrane transport components, are essential for this process. Although nuclear import and export for different...... transcription factor families are regulated by similar mechanisms, there are several differences that allow for the specific activation of each transcription factor. This review discusses the general import and export pathways found to be common amongst many different transcription factors, and highlights...

  12. Transcriptional Silencing of Retroviral Vectors

    DEFF Research Database (Denmark)

    Lund, Anders Henrik; Duch, M.; Pedersen, F.S.

    1996-01-01

    . Extinction of long-term vector expression has been observed after implantation of transduced hematopoietic cells as well as fibroblasts, myoblasts and hepatocytes. Here we review the influence of vector structure, integration site and cell type on transcriptional silencing. While down-regulation of proviral...... transcription is known from a number of cellular and animal models, major insight has been gained from studies in the germ line and embryonal cells of the mouse. Key elements for the transfer and expression of retroviral vectors, such as the viral transcriptional enhancer and the binding site for the t......RNA primer for reverse transcription may have a major influence on transcriptional silencing. Alterations of these elements of the vector backbone as well as the use of internal promoter elements from housekeeping genes may contribute to reduce transcriptional silencing. The use of cell culture and animal...

  13. DNA topology and transcription.

    Science.gov (United States)

    Kouzine, Fedor; Levens, David; Baranello, Laura

    2014-01-01

    Chromatin is a complex assembly that compacts DNA inside the nucleus while providing the necessary level of accessibility to regulatory factors conscripted by cellular signaling systems. In this superstructure, DNA is the subject of mechanical forces applied by variety of molecular motors. Rather than being a rigid stick, DNA possesses dynamic structural variability that could be harnessed during critical steps of genome functioning. The strong relationship between DNA structure and key genomic processes necessitates the study of physical constrains acting on the double helix. Here we provide insight into the source, dynamics, and biology of DNA topological domains in the eukaryotic cells and summarize their possible involvement in gene transcription. We emphasize recent studies that might inspire and impact future experiments on the involvement of DNA topology in cellular functions.

  14. The post-transcriptional operon

    DEFF Research Database (Denmark)

    Tenenbaum, Scott A.; Christiansen, Jan; Nielsen, Henrik

    2011-01-01

    A post-transcriptional operon is a set of monocistronic mRNAs encoding functionally related proteins that are co-regulated by a group of RNA-binding proteins and/or small non-coding RNAs so that protein expression is coordinated at the post-transcriptional level. The post-transcriptional operon...... model (PTO) is used to describe data from an assortment of methods (e.g. RIP-Chip, CLIP-Chip, miRNA profiling, ribosome profiling) that globally address the functionality of mRNA. Several examples of post-transcriptional operons have been documented in the literature and demonstrate the usefulness...

  15. Mastering Transcription: Multiplexed Analysis of Transcription Start Site Sequences.

    Science.gov (United States)

    Hochschild, Ann

    2015-12-17

    In this issue of Molecular Cell, Vvedenskaya et al. (2015) describe a high-throughput sequencing-based methodology for the massively parallel analysis of transcription from a high-complexity barcoded template library both in vitro and in vivo, providing a powerful new tool for the study of transcription.

  16. French Training

    CERN Multimedia

    Françoise Benz

    2003-01-01

    General and Professional French Courses The next session will take place: from 13 October to 19 December 2003. These courses are open to all persons working on the CERN site, and to their spouses. For registration and further information on the courses, please consult our Web pages or contact Mrs. Fontbonne: Tel. 72844. Writing Professional Documents in French This course is designed for people with a good level of spoken French. Duration: 30 hours Price: 660 CHF (for 8 students) For further information and registration, please consult our Web pages or contact Mrs. Fontbonne: Tel. 72844. Language Training Françoise Benz Tel.73127 language.training@cern.ch

  17. Transcriptional and post-transcriptional profile of human chromosome 21.

    Science.gov (United States)

    Nikolaev, Sergey I; Deutsch, Samuel; Genolet, Raphael; Borel, Christelle; Parand, Leila; Ucla, Catherine; Schütz, Frederic; Duriaux Sail, Genevieve; Dupré, Yann; Jaquier-Gubler, Pascale; Araud, Tanguy; Conne, Beatrice; Descombes, Patrick; Vassalli, Jean-Dominique; Curran, Joseph; Antonarakis, Stylianos E

    2009-08-01

    Recent studies have demonstrated extensive transcriptional activity across the human genome, a substantial fraction of which is not associated with any functional annotation. However, very little is known regarding the post-transcriptional processes that operate within the different classes of RNA molecules. To characterize the post-transcriptional properties of expressed sequences from human chromosome 21 (HSA21), we separated RNA molecules from three cell lines (GM06990, HeLa S3, and SK-N-AS) according to their ribosome content by sucrose gradient fractionation. Polyribosomal-associated RNA and total RNA were subsequently hybridized to genomic tiling arrays. We found that approximately 50% of the transcriptional signals were located outside of annotated exons and were considered as TARs (transcriptionally active regions). Although TARs were observed among polysome-associated RNAs, RT-PCR and RACE experiments revealed that approximately 40% were likely to represent nonspecific cross-hybridization artifacts. Bioinformatics discrimination of TARs according to conservation and sequence complexity allowed us to identify a set of high-confidence TARs. This set of TARs was significantly depleted in the polysomes, suggesting that it was not likely to be involved in translation. Analysis of polysome representation of RefSeq exons showed that at least 15% of RefSeq transcripts undergo significant post-transcriptional regulation in at least two of the three cell lines tested. Among the regulated transcripts, enrichment analysis revealed an over-representation of genes involved in Alzheimer's disease (AD), including APP and the BACE1 protease that cleaves APP to produce the pathogenic beta 42 peptide. We demonstrate that the combination of RNA fractionation and tiling arrays is a powerful method to assess the transcriptional and post-transcriptional properties of genomic regions.

  18. Language training

    CERN Multimedia

    2006-01-01

    If you wish to participate in one of the following courses, please tell to your supervisor and apply electronically from the course description pages that can be found on the Web at: http://www.cern.ch/Training/ or fill in an 'application for training' form available from your Departmental Secretariat or from your DTO (Departmental Training Officer). Applications will be accepted in the order in which they are received. General and Professional English Courses The next session will take place from beginning of October 2006 to beginning of February 2007 (3 weeks break at Christmas).These courses are open to all persons working on the CERN site and to their spouses. For registration and further information on the courses, please consult our Web pages: http://cern.ch/Training or contact Tessa Osborne, tel.16 23 40. Oral Expression The next session will take place from beginning of October 2006 to beginning of February 2007 (3 weeks break at Christmas).This course is intended for people with a good knowl...

  19. Language Training

    CERN Multimedia

    Françoise Benz

    2005-01-01

    If you wish to participate in one of the following courses, please tell to your supervisor and apply electronically from the course description pages that can be found on the Web at: http://www.cern.ch/Training/ or fill in an 'application for training' form available from your Departmental Secretariat or from your DTO (Departmental Training Officer). Applications will be accepted in the order in which they are received. General and Professional English Courses The next session will take place: from end of September 2005 to middle of February 2006 (2/ 3 weeks break at Christmas). These courses are open to all persons working on the CERN site, and to their spouses. For registration and further information on the courses, please consult our Web pages: http://cern.ch/Training or contact Mr. Liptow: tel. 72957. Oral Expression The next session will take place from end of September to December 2005. This course is intended for people with a good knowledge of English who want to practise and maintain the...

  20. Language training

    CERN Multimedia

    Françoise Benz

    2006-01-01

    If you wish to participate in one of the following courses, please tell to your supervisor and apply electronically from the course description pages that can be found on the Web at: http://www.cern.ch/Training/ or fill in an 'application for training'form available from your Departmental Secretariat or from your DTO (Departmental Training Officer). Applications will be accepted in the order in which they are received. General and Professional English Courses The next session will take place from beginning of October 2006 to beginning of February 2007 (3 weeks break at Christmas).These courses are open to all persons working on the CERN site and to their spouses. For registration and further information on the courses, please consult our Web pages: http://cern.ch/Training or contact Tessa Osborne, tel.16 23 40. Oral Expression The next session will take place from beginning of October 2006 to beginning of February 2007 (3 weeks break at Christmas).This course is intended for people with a goo...

  1. Technical Training: Technical Training Seminar

    CERN Multimedia

    2004-01-01

    TECHNICAL TRAINING Monique Duval tel. 74924 technical.training@cern.ch Monday 9 February 2004 From 10:00 to 12:00 - IT Auditorium - bldg. 31, 3rd floor ANSOFT High-Frequency Seminar David Prestaux, Application Engineer, ANSOFT F-78535 BUC, France This Technical Training seminar will present two Ansoft application products: Ansoft HFSS and Ansoft Designer. Ansoft HFSS makes use of the Finite Element Method (FEM) to calculate field solutions from first principles. It can accurately predict all high-frequency behaviours such as dispersion, mode conversion, and losses due to materials and radiation. Ansoft Designer is a suite of design tools to fully integrate high-frequency, physics-based electromagnetic simulations into a seamless system-level simulation environment. Ansoft Designer uses a simple interface to give complete control over every design task, by a method allowing multiple solvers, Solver on Demand. • Introduction • Overview of the Ansoft Total solution • Ansoft HFSS 9...

  2. Transcriptional networks controlling adipocyte differentiation

    DEFF Research Database (Denmark)

    Siersbæk, R; Mandrup, Susanne

    2011-01-01

    Adipocyte differentiation is regulated by a complex cascade of signals that drive the transcriptional reprogramming of the fibroblastic precursors. Genome-wide analyses of chromatin accessibility and binding of adipogenic transcription factors make it possible to generate "snapshots" of the trans...

  3. Sports Training

    Science.gov (United States)

    1988-01-01

    Practitioners of martial arts have long seen a need for a precise method of measuring the power of a karate kick or a boxer's punch in training and competition. Impax sensor is a piezoelectric film less than one thousandth of an inch thick, yet extremely durable. They give out a voltage impulse when struck, the greater the force of impact, the higher the voltage. The impulse is transmitted to a compact electronics package where voltage is translated into a force-pounds reading shown on a digital display. Impax, manufactured by Impulse Technology, Inc. is used by martial arts instructors, practitioners, U.S. Olympic Committee Training Center, football blocking sleds, and boxers as well as police defensive tactics, providing a means of evaluating the performance of recruits.

  4. Language Training

    CERN Multimedia

    HR Department

    Permanence A "permanence" for language Training has been set up. If anyone has a question or requires information on any aspect of English or French training please come to our office 5 4-016 at the following times. Lucette Fournier - French courses Monday 13.30 - 15.30 Tuesday\t10.30 - 12.30 Tessa Osborne - English courses Wednesday\t12.00 - 14.00 Thursday\t11.00 - 13.00   New courses Specific English and French courses - Exam preparation/ We are now offering specific courses in English and French leading to a recognised external examination (e.g. Cambridge, DELF, DALF). If you are interested in following one of these courses and have at least an upper intermediate level of English or French, please enrol through the following link:  English courses French courses Or contact: Tessa Osborne 72957 (English courses) Lucette Fournier 73483 (French courses) Language Training Nathalie Dumeaux Tel. 78144 nathalie.dumeaux@cern.ch

  5. Language Training

    CERN Multimedia

    HR Department

    2009-01-01

    PermanenceA "permanence" for language Training has been set up. If anyone has a question or requires information on any aspect of English or French training please come to our office 5 4-016 at the following times. Lucette Fournier French courses Monday 13.30 - 15.30 Tuesday\t10.30 - 12.30 Tessa Osborne English courses Wednesday\t12.00 - 14.00 Thursday\t11.00 - 13.00 New courses Specific English and French courses - Exam preparation/ We are now offering specific courses in English and French leading to a recognised external examination (e.g. Cambridge, DELF and BULATS). If you are interested in following one of these courses and have at least an upper intermediate level of English or French, please enrol through the following link: http://English courses http://French courses Or contact: Tessa Osborne 72957 (English courses) Lucette Fournier 73483 (French courses) Language Training Nathalie Dumeaux Tel. 78144 mailto:nathalie.dumeaux@cern.ch

  6. Sigma Factors for Cyanobacterial Transcription

    Directory of Open Access Journals (Sweden)

    Sousuke Imamura

    2009-04-01

    Full Text Available Cyanobacteria are photosynthesizing microorganisms that can be used as a model for analyzing gene expression. The expression of genes involves transcription and translation. Transcription is performed by the RNA polymerase (RNAP holoenzyme, comprising a core enzyme and a sigma (σ factor which confers promoter selectivity. The unique structure, expression, and function of cyanobacterial σ factors (and RNAP core subunits are summarized here based on studies, reported previously. The types of promoter recognized by the σ factors are also discussed with regard to transcriptional regulation.

  7. Transcription regulation mechanisms of bacteriophages

    Science.gov (United States)

    Yang, Haiquan; Ma, Yingfang; Wang, Yitian; Yang, Haixia; Shen, Wei; Chen, Xianzhong

    2014-01-01

    Phage diversity significantly contributes to ecology and evolution of new bacterial species through horizontal gene transfer. Therefore, it is essential to understand the mechanisms underlying phage-host interactions. After initial infection, the phage utilizes the transcriptional machinery of the host to direct the expression of its own genes. This review presents a view on the transcriptional regulation mechanisms of bacteriophages, and its contribution to phage diversity and classification. Through this review, we aim to broaden the understanding of phage-host interactions while providing a reference source for researchers studying the regulation of phage transcription. PMID:25482231

  8. RNA-guided transcriptional regulation

    Energy Technology Data Exchange (ETDEWEB)

    Church, George M.; Mali, Prashant G.; Esvelt, Kevin M.

    2016-02-23

    Methods of modulating expression of a target nucleic acid in a cell are provided including introducing into the cell a first foreign nucleic acid encoding one or more RNAs complementary to DNA, wherein the DNA includes the target nucleic acid, introducing into the cell a second foreign nucleic acid encoding a nuclease-null Cas9 protein that binds to the DNA and is guided by the one or more RNAs, introducing into the cell a third foreign nucleic acid encoding a transcriptional regulator protein or domain, wherein the one or more RNAs, the nuclease-null Cas9 protein, and the transcriptional regulator protein or domain are expressed, wherein the one or more RNAs, the nuclease-null Cas9 protein and the transcriptional regulator protein or domain co-localize to the DNA and wherein the transcriptional regulator protein or domain regulates expression of the target nucleic acid.

  9. Technical Training: Technical Training Seminar

    CERN Multimedia

    2004-01-01

    TECHNICAL TRAINING Monique Duval tel. 74924 technical.training@cern.ch Tuesday 3 February 2004 From 09:00 to 13:30 - Training Centre Auditorium - bldg. 593, room 11 USB (Universal Serial Bus) CYPRESS Seminar Claudia Colombini, Field Application Engineer CYPRESS ActiveComp Electronic GmbH D-85077 MANCHING, Germany As a pioneer in USB, CYPRESS sets the standard for cost-effective solutions without sacrificing functionality, performance or reliability. Having shipped over 200 million USB devices, Cypress is the undisputed market leader and demonstrates unmatched USB expertise. With the industry's broadest selection of USB solutions, Cypress has the right silicon, software and support for every USB application, from Low-speed to High-Speed and USB On-The-Go (OTG). 9:00 - 10:30 Overview of USB systems. USB CYPRESS product overview. Peripherals: Low Speed, Full Speed, High Speed (1.1 and 2.0). Hub Solutions, Embedded Host Solutions, On-The-Go (OTG) and wireless USB. USB Development Tools (first part) 10:30 -...

  10. National Capital Planning Commission Meeting Transcripts

    Data.gov (United States)

    National Capital Planning Commission — Transcripts of the monthly (with the exception of August) National Capital Planning Commission meeting transcripts are provided for research to confirm actions taken...

  11. Strength Training. A Key to Athletic Training.

    Science.gov (United States)

    Whiteside, Patricia W.

    Characteristics of an effective strength training program are analyzed and descriptions are offered of different kinds of weight training activities. Comparisons are made between concentric, isometric, eccentric, and isokinetic training methods. The fundamentals and techniques of an exemplary training program are outlined and the organization and…

  12. SELECTING AND TRAINING THE TRAINING OFFICER.

    Science.gov (United States)

    TAYLOR, NANCY

    TO ACHIEVE THE OBJECTIVES OF TRAINING IN INDUSTRY--TECHNICAL AND LIBERAL EDUCATION, SPECIFIC JOB SKILLS, AND THE DEVELOPMENT OF ATTITUDES--THE TRAINING OFFICER MUST KNOW THE COMPANY WITHIN WHICH HE IS WORKING, AS WELL AS MANAGEMENT THEORY AND TRAINING METHODS. THE SELECTION OF TRAINING OFFICERS IS BASED ON A JOB SPECIFICATION, AN OUTGROWTH OF A…

  13. ACADEMIC TRAINING

    CERN Multimedia

    Françoise Benz

    2002-01-01

    6, 7 May LECTURE SERIES from 11.00 to 12.00 hrs - Auditorium, bldg. 500 Decoding the Human Genome, Scientific basis and ethic and social aspects by S.E. Antonarakis and A. Mauron / Univ. of Geneva Decoding the Human genome is a very up-to-date topic, raising several questions besides purely scientific, in view of the two competing teams (public and private), the ethics of using the results, and the fact that the project went apparently faster and easier than expected. The lecture series will address the following chapters: Scientific basis and challenges, Ethical and social aspects of genomics. Academic Training Françoise Benz Tel. 73127

  14. Media Training

    CERN Document Server

    CERN. Geneva

    2009-01-01

    With the LHC starting up soon, the world's media are again turning their attention to CERN. We're all likely to be called upon to explain what is happening at CERN to media, friends and neighbours. The seminar will be given by BBC television news journalists Liz Pike and Nadia Marchant, and will deal with the kind of questions we're likely to be confronted with through the restart period. The training is open for everybody. Make sure you arrive early enough to get a seat - there are only 200 seats in the Globe. The session will also be webcast: http://webcast.cern.ch/

  15. Iterative Chat Transcript Analysis: Making Meaning from Existing Data

    Directory of Open Access Journals (Sweden)

    Steven Baumgart

    2016-04-01

    Full Text Available Objective – In order to better contextualize library data about patron satisfaction with reference services, we analyzed an existing corpus of chat transcripts. Having conducted a similar analysis in 2010, we also compared librarian behaviors over time. Methods – Drawing from the library literature, we identified a set of librarian behaviors closely associated with patron satisfaction. These behaviors include listening to and understanding patrons’ needs, inviting patrons to use the service again, and providing instruction or completing a search for patrons. Analysis of the chat transcripts included establishing a coding schema, applying these codes to individual chat transcripts, and analyzing these codes across the corpus of transcripts for frequency and correlation with other codes. The currently presented analysis used chat transcripts from the fall of 2013 and seeks changes in librarian behavior over time in order to gauge the success of establishing best practices and improving training standardization over the last three years. Results – The analysis shows that librarian behaviors have changed over time, pointing to what campus librarians are doing well, and that implementation of best practices at a campus level after the 2010 analysis may have increased these positive behaviors. The analysis also shows opportunities for further standardization and reinforcement of best practices. Conclusion – Qualitative analysis of already-collected data serves as a model for other units and suggests areas for process improvement, including enhanced coder training and code schema design. Further analysis of chat patrons’ questions is also warranted, including investigation of the relationship between subject- and location-specific questions and referrals.

  16. English Training

    CERN Multimedia

    2003-01-01

    General and Professional English Courses The next session will take place: from 03 March to 28 June 2003 (2 weeks break at Easter). These courses are open to all persons working on the Cern site, and to their spouses. For registration and further information on the courses, please consult our Web pages: http://cern.ch/Training or contact Mrs. Benz: tel.73127 or Mr. Liptow: tel.72957. Writing Professional Documents in English This course is designed for people with a good level of spoken English. Duration: 20 hours (2 hours a week) Price: 440 CHF (for 8 students) For registration and further information, please consult our Web pages: http://cern.ch/Training or contact Mrs. Tessa Osborne: Tessa.Osborne@cern. Oral Expression This course is intended for people with a good knowledge of English who want to practise and maintain their speaking skills while extending their vocabulary. There will be approximately 8 participants in a class. Speaking activities will include discussions, meeting simulations, role-p...

  17. Language Training

    CERN Multimedia

    2003-01-01

    Formation en anglais Cours d'anglais général et professionnel La prochaine session se déroulera: du 06 octobre 2003 au 13 février 2004 (interruption de 3/4 semaines à Noël). Ces cours s'adressent à toute personne travaillant au CERN ainsi qu'à leur conjoint. Pour vous inscrire et voir tout le détail des cours proposés, consultez nos pages Web: http://cern.ch/Training Vous pouvez aussi contacter Mme Benz, tél. 73127 ou Mr Liptow, tél. 72957. General and Professional English Courses The next session will take place: from 06 October 2003 to 13 February 2004 (3/4 weeks break at Christmas). These courses are open to all persons working on the Cern site, and to their spouses. For registration and further information on the courses, please consult our Web pages: http://cern.ch/Training or contact Mrs. Benz: tel. 73127 or Mr. Liptow: tel. 72957. Writing Professional Documents in English This course is designed for people with a good level of spoken English. Duration: 20 hours (2 hours a week) Price: ...

  18. Language Training

    CERN Multimedia

    2003-01-01

    Formation en anglais Cours d'anglais général et professionnel La prochaine session se déroulera: du 06 octobre 2003 au 13 février 2004 (interruption de 3/4 semaines à Noël). Ces cours s'adressent à toute personne travaillant au CERN ainsi qu'à leur conjoint. Pour vous inscrire et voir tout le détail des cours proposés, consultez nos pages Web: http://cern.ch/Training Vous pouvez aussi contacter Mme Benz, tél. 73127 ou Mr Liptow, tél. 72957. General and Professional English Courses The next session will take place: from 06 October 2003 to 13 February 2004 (3/4 weeks break at Christmas). These courses are open to all persons working on the Cern site, and to their spouses. For registration and further information on the courses, please consult our Web pages: http://cern.ch/Training or contact Mrs. Benz: tel. 73127 or Mr. Liptow: tel. 72957. Writing Professional Documents in Engl...

  19. English Training

    CERN Multimedia

    2003-01-01

    General and Professional English Courses The next session will take place: from 03 March to 28 June 2003 (2 weeks break at Easter). These courses are open to all persons working on the Cern site, and to their spouses. For registration and further information on the courses, please consult our Web pages: http://cern.ch/Training or contact Mrs. Benz: tel.73127 or Mr. Liptow: tel.72957. Writing Professional Documents in English This course is designed for people with a good level of spoken English. Duration: 20 hours (2 hours a week) Price: 440 CHF (for 8 students) For registration and further information, please consult our Web pages: http://cern.ch/Training or contact Mrs. Tessa Osborne: Tessa.Osborne@cern. Oral Expression This course is intended for people with a good knowledge of English who want to practise and maintain their speaking skills while extending their vocabulary. There will be approximately 8 participants in a class. Speaking activities will include discussions, meeting simulations, rol...

  20. Language Training

    CERN Multimedia

    2003-01-01

    Formation en anglais Cours d'anglais général et professionnel La prochaine session se déroulera: du 06 octobre 2003 au 13 février 2004 (interruption de 3/4 semaines à Noël). Ces cours s'adressent à toute personne travaillant au CERN ainsi qu'à leur conjoint. Pour vous inscrire et voir tout le détail des cours proposés, consultez nos pages Web: http://cern.ch/Training Vous pouvez aussi contacter M. Liptow, tél. 72957. General and Professional English Courses The next session will take place: from 06 October 2003 to 13 February 2004 (3/4 weeks break at Christmas). These courses are open to all persons working on the Cern site, and to their spouses. For registration and further information on the courses, please consult our Web pages: http://cern.ch/Training or contact Mr. Liptow: tel. 72957. Writing Professional Documents in English This course is designed for people with a good level of...

  1. French training

    CERN Multimedia

    2007-01-01

    The next session will take place from 29 January to 30 March 2007. These courses are open to all persons working on the CERN site, and their spouses. For registration and further information on the courses, please consult our Web pages: http://cern.ch/Training or contact Mrs. Benz: tel. 73127. Writing Professional Documents in French The next session will take place from 29 January to 30 March 2007. This course is designed for people with a good level of spoken French. Duration: 30 hours Price: 660 CHF (for 8 students) For further information and registration, please consult our Web pages: http://cern.ch/Training or contact Mrs. Benz: tel. 73127. Writing Professional Documents in English The next session will take place from January to June 2007 (break at Easter). This course is designed for people with a good level of spoken English. Duration: 30 hours Price: 660 CHF (for 8 students) Timetable will be fixed after discussion with the students. For further information and registration, please consu...

  2. Intermediate and long-term memories of associative learning are differentially affected by transcription versus translation blockers in Lymnaea.

    Science.gov (United States)

    Sangha, Susan; Scheibenstock, Andi; McComb, Chloe; Lukowiak, Ken

    2003-05-01

    Aerial respiratory behaviour in the pond snail, Lymnaea stagnalis, can be operantly conditioned. This associative learning then undergoes consolidation into a long-lasting memory which, depending on the training procedure used, causes intermediate-term memory (ITM; lasting 3 h) or long-term memory (LTM; lasting >6 h) to be formed. We determined the differential susceptibility of these two forms of memory to translation and transcription blockers. The injection of a translation blocker, Anisomycin, 2.5 h before training prevents the establishment of both ITM and LTM. On the other hand, injection of the transcription blocker Actinomycin D, 2.5 h before training, did not prevent the establishment of ITM, but did, however, prevent LTM formation. Thus in Lymnaea, following associative learning, both ITM and LTM are dependent on new protein synthesis. ITM appears to be dependent on protein synthesis from preexisting transcription factors, whilst LTM is dependent on protein synthesis from new transcription messages.

  3. Escherichia coli transcriptional regulatory network

    Directory of Open Access Journals (Sweden)

    Agustino Martinez-Antonio

    2011-06-01

    Full Text Available Escherichia coli is the most well-know bacterial model about the function of its molecular components. In this review are presented several structural and functional aspects of their transcriptional regulatory network constituted by transcription factors and target genes. The network discussed here represent to 1531 genes and 3421 regulatory interactions. This network shows a power-law distribution with a few global regulators and most of genes poorly connected. 176 of genes in the network correspond to transcription factors, which form a sub-network of seven hierarchical layers where global regulators tend to be set in superior layers while local regulators are located in the lower ones. There is a small set of proteins know as nucleoid-associated proteins, which are in a high cellular concentrations and reshape the nucleoid structure to influence the running of global transcriptional programs, to this mode of regulation is named analog regulation. Specific signal effectors assist the activity of most of transcription factors in E. coli. These effectors switch and tune the activity of transcription factors. To this type of regulation, depending of environmental signals is named the digital-precise-regulation. The integration of regulatory programs have place in the promoter region of transcription units where it is common to observe co-regulation among global and local TFs as well as of TFs sensing exogenous and endogenous conditions. The mechanistic logic to understand the harmonious operation of regulatory programs in the network should consider the globalism of TFs, their signal perceived, coregulation, genome position, and cellular concentration. Finally, duplicated TFs and their horizontal transfer influence the evolvability of members of the network. The most duplicated and transferred TFs are located in the network periphery.

  4. 针对微风发电的城市风场特性初步研究%A Preliminary Research of Urban Wind Farm Characteristics for the Breeze Power Generation

    Institute of Scientific and Technical Information of China (English)

    李登君; 韦雅文; 季丰

    2013-01-01

    近年来,风电行业在全球都获得了快速发展。但传统风电机组在城市环境中却难以正常工作。为了实现在城市中进行微风发电,就需要探究其原因,对城市风场特性进行分析和研究。本研究以南京作为代表城市,使用便携式风速仪和固定式气象站对城市环境中的风场特性进行了观测和分析。根据结果,总结出影响风力发电效果的城市风场特性主要有三大方面:低风速、频变风向和湍流。最后对照传统风力发电选址的技术标准提出城市风力发电的发展方向。%In recent years, the wind power industry in the world has achieved rapid growth. But traditional wind turbine is difcult to work properly in an urban environment. A preliminary study of urban wind farm characteristics was made in order to fnd out the reason and make the generation breeze in urban area to become reality. In this study, portable anemometer and fxed weather station were used to observe and analysis for the characteristics of urban wind farm in Nanjing city, which was made as representative. According to the measurement results, the three mainly infuencing factors of urban wind farm characteristics that impact the efect of wind power generation are:low wind velocity, frequent changing wind direction and turbulence. At last, the direction of development for urban wind power generation was put forward according to the technical standards of address selection for traditional wind power generation.

  5. Observation on the characteristics of sea-land breezes and its influence to air quality over Pearl River Delta region during dry season%珠三角干季海陆风特征及其对空气质量影响的观测

    Institute of Scientific and Technical Information of China (English)

    吴蒙; 罗云; 吴兑; 范绍佳

    2016-01-01

    Based on the sounding data obtained from the boundary layer observation experiment over Pearl River Delta (PRD) region during October 2004 and the corresponding hourly PM2.5 data, the characteristics of sea-land breezes and its influenceson quality over PRD region were discussed by Recirculation Factor (RF) and other methods. Recirculation factor was a useful tool to represent the atmospheric horizontal transmission capacity.With the influence of the cold air and warm air confront with each other during pollution days, the sea-land breezes occurred frequently as a result of the weak system wind, and air quality index had significant relation with the RF, the RF of 100~400m was about 0.5~0.8. The horizontal transmission capacity of vertical wind field was weak under the influence of sea-land breezes, not conducive to the spread of pollutants. As PRD controlled by cold air overall, the RF of vertical windreach up to 0.9, and sea-land breezes circulation was hard to developing,so the horizontal transmissions capacity was powerful and the pollutants could be spread effectually. Among the observations, the frequency of sea-land breezes was about 47.8%at the coastal station, and the 72.7%of sea-land breezes day was pollution day, the wind directionhad visible change in clockwise over time. The sea breeze occurred in 16:00, and reached the maximum in 20:00 with the influence height was about 600~800m. In the night, sea breeze may transport the pollutants back to inland, and gave rise to a peak value of PM2.5 concentration appeared at 19:00 to 21:00 in inland station.%通过2004年10月在珠江三角洲(以下简称“珠三角”)开展大气边界层观测试验得到的垂直风温资料和逐时PM2.5浓度资料,利用局地环流指数(RF)等方法研究了珠三角海陆风特征及其对空气质量的影响.结果表明:局地环流指数是表征局地大气输送能力的有效指标;冷暖气团对峙导致珠三角污染日背景风场较弱,沿海海

  6. "Training Floors" and "Training Ceilings": Metonyms for Understanding Training Trends

    Science.gov (United States)

    Felstead, Alan; Jewson, Nick

    2014-01-01

    This article outlines a conceptual framework for mapping and understanding training trends. It uses the metonyms of floors and ceilings to distinguish between different types of training configurations. The argument is made that the ups and downs of employer reports of training activity are a crude basis on which to make judgements about the…

  7. English training

    CERN Multimedia

    2003-01-01

    You have a good level of English BUT... You still need to improve your speaking or You have problems writing professional documents Would you like to work in a small group on either of these areas? Then, the following courses are for you! Writing Professional Documents in English The aim of the course is for students to improve their professional writing. Participants will work on technical, scientific or administrative documents depending on the needs of the group. Duration: 20 hours (2 hours a week) Price: 440 CHF (for 8 students) Oral Expression The emphasis will be on oral expression with necessary feed-back. Speaking activities will include discussions, meeting simulations, role-plays etc. Duration: 20 hours (2 hours a week) Price: 440 CHF (for 8 students) For registration and further information, please consult our Web pages: http://cern.ch/Training or contact Mr. Liptow: tel. 72957 / Mrs. Tessa Osborne: Tessa.Osborne@cern.ch.

  8. English Training

    CERN Multimedia

    2003-01-01

    You have a good level of English BUT... You still need to improve your speaking or You have problems writing professional documents Would you like to work in a small group on either of these areas? Then, the following courses are for you! Writing Professional Documents in English The aim of the course is for students to improve their professional writing. Participants will work on technical, scientific or administrative documents depending on the needs of the group. Duration: 20 hours (2 hours a week) Price: 440 CHF (for 8 students) Oral Expression The emphasis will be on oral expression with necessary feed-back. Speaking activities will include discussions, meeting simulations, role-plays etc. Duration: 20 hours (2 hours a week) Price: 440 CHF (for 8 students) For registration and further information, please consult our Web pages: http://cern.ch/Training or contact Mr. Liptow: tel. 72957 / Mrs. Tessa Osborne: Tessa.Osborne@cern.ch.

  9. ACADEMIC TRAINING

    CERN Multimedia

    Françoise Benz

    2002-01-01

    25, 26, 27, 28 February and 1st March from 11.00 to 12.00 hrs - Auditorium, bldg. 500 LECTURE SERIES Neutrino masses and oscillations by A. de Rujula / CERN-TH This course will not cover its subject in the customary way. The emphasis will be on the simple theoretical concepts (helicity, handedness, chirality, Majorana masses) which are obscure in most of the literature, and on the quantum mechanics of oscillations, that ALL books get wrong. Which, hopefully, will not deter me from discussing some of the most interesting results from the labs and from the cosmos. Academic Training Françoise Benz Secretariat Tel. 73127 francoise.benz@cern.ch

  10. Nuclear manpower training

    Energy Technology Data Exchange (ETDEWEB)

    Suh, In Suk; Lee, H. Y.; Joe, B. J.; Lee, S. H.; Lee, E. J.; Yoo, B. H.; Seo, K. W.; Lee, W. K.; Jun, H. I.; Yang, K. N.; Kim, Y. J.; Kim, I. H.; Kim, M. Y.; Ju, Y. C.; Hyun, H. Y.; Choi, I. G.; Hong, C. S.; Won, J. Y.; Nam, J. Y.; Lee, H. J.

    1997-01-01

    This report describes the annual results of training courses. the scope and contents are as follows: 1. Regional and interregional training courses 2. Training courses assisted by foreign experts 3. Training courses for nuclear industry personnel 4. Training courses for internal staff-members 5. Training courses under the law. This Nuclear Training Center executed the open-door training courses for 2,400 engineers/scientists from the regulatory body, nuclear industries, research institutes and other related organizations by means of offering 51 training courses during the fiscal year 1996. (author). 23 refs.

  11. Pervasive transcription: detecting functional RNAs in bacteria.

    Science.gov (United States)

    Lybecker, Meghan; Bilusic, Ivana; Raghavan, Rahul

    2014-01-01

    Pervasive, or genome-wide, transcription has been reported in all domains of life. In bacteria, most pervasive transcription occurs antisense to protein-coding transcripts, although recently a new class of pervasive RNAs was identified that originates from within annotated genes. Initially considered to be non-functional transcriptional noise, pervasive transcription is increasingly being recognized as important in regulating gene expression. The function of pervasive transcription is an extensively debated question in the field of transcriptomics and regulatory RNA biology. Here, we highlight the most recent contributions addressing the purpose of pervasive transcription in bacteria and discuss their implications.

  12. Subventricular zone microglia transcriptional networks.

    Science.gov (United States)

    Starossom, Sarah C; Imitola, Jaime; Wang, Yue; Cao, Li; Khoury, Samia J

    2011-07-01

    Microglia play an important role in inflammatory diseases of the central nervous system. There is evidence of microglial diversity with distinct phenotypes exhibiting either neuroprotection and repair or neurotoxicity. However the precise molecular mechanisms underlying this diversity are still unknown. Using a model of experimental autoimmune encephalomyelitis (EAE) we performed transcriptional profiling of isolated subventricular zone microglia from the acute and chronic disease phases of EAE. We found that microglia exhibit disease phase specific gene expression signatures, that correspond to unique gene ontology functions and genomic networks. Our data demonstrate for the first time, distinct transcriptional networks of microglia activation in vivo, that suggests a role as mediators of injury or repair.

  13. NAC transcription factors in senescence

    DEFF Research Database (Denmark)

    Podzimska-Sroka, Dagmara; O'Shea, Charlotte; Gregersen, Per L.;

    2015-01-01

    Within the last decade, NAC transcription factors have been shown to play essential roles in senescence, which is the focus of this review. Transcriptome analyses associate approximately one third of Arabidopsis NAC genes and many crop NAC genes with senescence, thereby implicating NAC genes...... as important regulators of the senescence process. The consensus DNA binding site of the NAC domain is used to predict NAC target genes, and protein interaction sites can be predicted for the intrinsically disordered transcription regulatory domains of NAC proteins. The molecular characteristics...

  14. Spaced cognitive training promotes training transfer

    Directory of Open Access Journals (Sweden)

    Zuowei eWang

    2014-04-01

    Full Text Available Cognitive training studies yield wildly inconsistent results. One dimension on which studies vary is the scheduling of training sessions (Morrison & Chein, 2011. In this study, we systematically address whether or not spacing of practice influences training and transfer. We randomly assigned 115 fifth grade children to an active control group or one of four training groups who received working memory training based on a running span task (Zhao et al., 2011. All groups received the same total amount of training: 20 sessions of training with 60 trials for an average of 20 minutes per session. The training was spread across 2 days, 5 days, 10 days, or 20 days. The active control group received 20-minute sessions of math instruction for 20 sessions. Before and after training participants in all five groups performed a single transfer test that assessed fluid intelligence, the Raven's Progressive Matrices Test. Overall, participants in all four training groups improved significantly on the training task (at least partially, as reflected by increased speed. More importantly, the only training group to show significant improvement on the Raven's was the group who had the greatest amount of spacing (20 days group during training and improvement in this group was significantly higher than that of the control group.

  15. Spaced cognitive training promotes training transfer.

    Science.gov (United States)

    Wang, Zuowei; Zhou, Renlai; Shah, Priti

    2014-01-01

    Cognitive training studies yield wildly inconsistent results. One dimension on which studies vary is the scheduling of training sessions (Morrison and Chein, 2011). In this study, we systematically address whether or not spacing of practice influences training and transfer. We randomly assigned 115 fifth grade children to an active control group or one of four training groups who received working memory training based on a "running span" task (Zhao et al., 2011). All groups received the same total amount of training: 20 sessions of training with 60 trials for an average of 20 min per session. The training was spread across 2, 5, 10, or 20 days. The active control group received 20-min sessions of math instruction for 20 sessions. Before and after training participants in all five groups performed a single transfer test that assessed fluid intelligence, the Raven's Progressive Matrices Test. Overall, participants in all four training groups improved significantly on the training task (at least partially), as reflected by increased speed. More importantly, the only training group to show significant improvement on the Raven's was the group who had the greatest amount of spacing (20 days group) during training and improvement in this group was significantly higher than that of the control group.

  16. Training the Sales Neophyte

    Science.gov (United States)

    Harris, Clyde E., Jr.

    1975-01-01

    The article reappraises initial sales training and presents a program emphasizing objectives, responsibility for training, program content, and teaching techniques. Formal Initial Responsive Sales Training System (FIRSTS) is the name of the program explored and evaluated. (Author/MW)

  17. Toilet training tips

    Science.gov (United States)

    Potty training ... show signs that they are ready for toilet training between ages 18 and 30 months. Before 18 ... way that she is ready to start toilet training. Children are ready when they: Show interest in ...

  18. Language training

    CERN Multimedia

    2007-01-01

    General and Professional English Courses The next session will take place from 26 February or 5 March to end of June 2007 (1/2 weeks break at Easter). These courses are open to all persons working on the CERN site, and to their spouses. For registration and further information on the courses, please consult our Web pages: http://cern.ch/Training or contact Françoise Benz, tel. 73127. Oral Expression The next session will take place from 26 February or 5 March to end of June 2007 (1/2 weeks break at Easter). This course is intended for people with a good knowledge of English who want to practise and maintain their speaking skills while extending their vocabulary. There will be a maximum of 10 participants in a class. Speaking activities will include discussions, meeting simulations, role-plays etc. depending on the needs of the students. Duration: 30 hours Price: 660 CHF (for a minimum of 8 students). Writing Professional Documents in English The next session will take place from 26 February or...

  19. Language training

    CERN Multimedia

    Françoise Benz

    2005-01-01

    General and Professional English Courses The next session will take place: from end of September 2005 to middle of February 2006 (2/ 3 weeks break at Christmas). These courses are open to all persons working on the CERN site, and to their spouses. For registration and further information on the courses, please consult our Web pages: http://cern.ch/Training or contact Mr. Liptow: tel. 72957. Oral Expression in English The next session will take place from end of September to December 2005. This course is intended for people with a good knowledge of English who want to practise and maintain their speaking skills while extending their vocabulary. There will be a maximum of 10 participants in a class. Speaking activities will include discussions, meeting simulations, role-plays etc. depending on the needs of the students. Duration: 20 hours Price: 440 CHF (for a minimum of 8 students) Writing Professional Documents in English The next session will take place from end of September 2005 to middle of Feb...

  20. Language training

    CERN Multimedia

    2007-01-01

    General and Professional English Courses The next session will take place: from 26 February or 5 March to end of June 2007 (1/2 weeks break at Easter). These courses are open to all persons working on the CERN site, and to their spouses. For registration and further information on the courses, please consult our Web pages: http://cern.ch/Training or contact Françoise Benz, tel. 73127. Oral Expression The next session will take place from 26 February or 5 March to end of June 2007 (1/2 weeks break at Easter). This course is intended for people with a good knowledge of English who want to practise and maintain their speaking skills while extending their vocabulary. There will be a maximum of 10 participants in a class. Speaking activities will include discussions, meeting simulations, role-plays etc. depending on the needs of the students. Duration: 30 hours Price: 660 CHF (for a minimum of 8 students). Writing Professional Documents in English The next session will take place from 26 February or 5 M...

  1. Language Training

    CERN Multimedia

    Françoise Benz

    2005-01-01

    General and Professional English Courses The next session will take place: from end of September 2005 to middle of February 2006 (2/ 3 weeks break at Christmas). These courses are open to all persons working on the CERN site, and to their spouses. For registration and further information on the courses, please consult our Web pages: http://cern.ch/Training or contact Mr. Liptow: tel. 72957. Oral Expression in English The next session will take place from end of September to December 2005. This course is intended for people with a good knowledge of English who want to practise and maintain their speaking skills while extending their vocabulary. There will be a maximum of 10 participants in a class. Speaking activities will include discussions, meeting simulations, role-plays etc. depending on the needs of the students. Duration: 20 hours Price: 440 CHF (for a minimum of 8 students) Writing Professional Documents in English The next session will take place from end of September 2005 to middle of Febr...

  2. Language training

    CERN Multimedia

    2006-01-01

    General and Professional English Courses The next session will take place from beginning of October 2006 to beginning of February 2007 (3 weeks break at Christmas).These courses are open to all persons working on the CERN site and to their spouses. For registration and further information on the courses, please consult our Web pages: http://cern.ch/Training or contact Tessa Osborne, tel.16 23 40. Oral Expression The next session will take place from beginning of October 2006 to beginning of February 2007 (3 weeks break at Christmas).This course is intended for people with a good knowledge of English who want to practise and maintain their speaking skills while extending their vocabulary. There will be a maximum of 10 participants in a class. Speaking activities will include discussions, meeting simulations, role-plays etc., depending on the needs of the students. Duration: 30 hours (2 hours a week) Price: 660 CHF (for a minimum of 8 students). Writing Professional Documents in English The next ses...

  3. Transcription factors in alkaloid biosynthesis.

    Science.gov (United States)

    Yamada, Yasuyuki; Sato, Fumihiko

    2013-01-01

    Higher plants produce a large variety of low-molecular weight secondary compounds. Among them, nitrogen-containing alkaloids are the most biologically active and are often used pharmaceutically. Whereas alkaloid chemistry has been intensively investigated, alkaloid biosynthesis, including the relevant biosynthetic enzymes, genes and their regulation, and especially transcription factors, is largely unknown, as only a limited number of plant species produce certain types of alkaloids and they are difficult to study. Recently, however, several groups have succeeded in isolating the transcription factors that are involved in the biosynthesis of several types of alkaloids, including bHLH, ERF, and WRKY. Most of them show Jasmonate (JA) responsiveness, which suggests that the JA signaling cascade plays an important role in alkaloid biosynthesis. Here, we summarize the types and functions of transcription factors that have been isolated in alkaloid biosynthesis, and characterize their similarities and differences compared to those in other secondary metabolite pathways, such as phenylpropanoid and terpenoid biosyntheses. The evolution of this biosynthetic pathway and regulatory network, as well as the application of these transcription factors to metabolic engineering, is discussed.

  4. Transcriptional networks in plant immunity.

    Science.gov (United States)

    Tsuda, Kenichi; Somssich, Imre E

    2015-05-01

    Next to numerous abiotic stresses, plants are constantly exposed to a variety of pathogens within their environment. Thus, their ability to survive and prosper during the course of evolution was strongly dependent on adapting efficient strategies to perceive and to respond to such potential threats. It is therefore not surprising that modern plants have a highly sophisticated immune repertoire consisting of diverse signal perception and intracellular signaling pathways. This signaling network is intricate and deeply interconnected, probably reflecting the diverse lifestyles and infection strategies used by the multitude of invading phytopathogens. Moreover it allows signal communication between developmental and defense programs thereby ensuring that plant growth and fitness are not significantly retarded. How plants integrate and prioritize the incoming signals and how this information is transduced to enable appropriate immune responses is currently a major research area. An important finding has been that pathogen-triggered cellular responses involve massive transcriptional reprogramming within the host. Additional key observations emerging from such studies are that transcription factors (TFs) are often sites of signal convergence and that signal-regulated TFs act in concert with other context-specific TFs and transcriptional co-regulators to establish sensory transcription regulatory networks required for plant immunity.

  5. Transcription factor-based biosensor

    Science.gov (United States)

    Dietrich, Jeffrey A; Keasling, Jay D

    2013-10-08

    The present invention provides for a system comprising a BmoR transcription factor, a .sigma..sup.54-RNA polymerase, and a pBMO promoter operatively linked to a reporter gene, wherein the pBMO promoter is capable of expression of the reporter gene with an activated form of the BmoR and the .sigma..sup.54-RNA polymerase.

  6. Dynamic training devices in CRM training

    Science.gov (United States)

    Lawver, J.

    1984-01-01

    Pilot training effectiveness and flying safety of a seasonal tour flight company are described. The change from single pilot to two pilot operated twin otters is examined. The use of the ATC 810 training device, its possibilities and training capacity is outlined. Problem areas which may arise, emergency system and pilot/passenger interaction are analyzed.

  7. Ensemble approach combining multiple methods improves human transcription start site prediction.

    LENUS (Irish Health Repository)

    Dineen, David G

    2010-01-01

    The computational prediction of transcription start sites is an important unsolved problem. Some recent progress has been made, but many promoters, particularly those not associated with CpG islands, are still difficult to locate using current methods. These methods use different features and training sets, along with a variety of machine learning techniques and result in different prediction sets.

  8. Stepwise mechanism for transcription fidelity

    Directory of Open Access Journals (Sweden)

    Zorov Savva

    2010-05-01

    Full Text Available Abstract Background Transcription is the first step of gene expression and is characterized by a high fidelity of RNA synthesis. During transcription, the RNA polymerase active centre discriminates against not just non-complementary ribo NTP substrates but also against complementary 2'- and 3'-deoxy NTPs. A flexible domain of the RNA polymerase active centre, the Trigger Loop, was shown to play an important role in this process, but the mechanisms of this participation remained elusive. Results Here we show that transcription fidelity is achieved through a multi-step process. The initial binding in the active centre is the major discrimination step for some non-complementary substrates, although for the rest of misincorporation events discrimination at this step is very poor. During the second step, non-complementary and 2'-deoxy NTPs are discriminated against based on differences in reaction transition state stabilization and partly in general base catalysis, for correct versus non-correct substrates. This step is determined by two residues of the Trigger Loop that participate in catalysis. In the following step, non-complementary and 2'-deoxy NTPs are actively removed from the active centre through a rearrangement of the Trigger Loop. The only step of discrimination against 3'-deoxy substrates, distinct from the ones above, is based on failure to orient the Trigger Loop catalytic residues in the absence of 3'OH. Conclusions We demonstrate that fidelity of transcription by multi-subunit RNA polymerases is achieved through a stepwise process. We show that individual steps contribute differently to discrimination against various erroneous substrates. We define the mechanisms and contributions of each of these steps to the overall fidelity of transcription.

  9. Fanning the Optimal Breeze with an Abanico

    Science.gov (United States)

    Goon, Grace; Marthelot, Joel; Reis, Pedro; MIT EGS Lab Team

    Flexible hand-held fans, or abanicos, are universally employed as cooling devices that are both portable and sustainable. Their to and fro axial motion about one's hand generates an airflow that increases the evaporation rate near the skin and refreshes. We study this problem in the context of fluid-structure interaction, through precision model experiments. We first characterize the elastic properties of a semi-circular thin plates with various thickness and evaluate their aerodynamic performance in a custom built apparatus. The air velocity profile that results from the flapping motion of the fan is characterized for different driving conditions. A systematic variation of the geometric and elastic parameters, along with an exploration of the parameter space of the periodic driving motion (amplitude and frequency), allows us to establish optimal design and operational conditions for maximal output of the generated airflow, while minimizing the input power.

  10. Sea breeze generated waves and coastal morphology

    NARCIS (Netherlands)

    Verhagen, H.J.; Savov, B.

    1999-01-01

    For the determination of the stability of coastlines, coastal erosion and the design of erosion protection studies, the “local” wave climate is the most important input parameter. For morphology, “local” means just outside the breaker line. On relatively calm days the local wave climate is strongly

  11. Analysis of Phonetic Transcriptions for Danish Automatic Speech Recognition

    DEFF Research Database (Denmark)

    Kirkedal, Andreas Søeborg

    2013-01-01

    recognition system depends heavily on the dictionary and the transcriptions therein. This paper presents an analysis of phonetic/phonemic features that are salient for current Danish ASR systems. This preliminary study consists of a series of experiments using an ASR system trained on the DK-PAROLE corpus....... The analysis indicates that transcribing e.g. stress or vowel duration has a negative impact on performance. The best performance is obtained with coarse phonetic annotation and improves performance 1% word error rate and 3.8% sentence error rate....

  12. Investigating transcription reinitiation through in vitro approaches.

    Science.gov (United States)

    Dieci, Giorgio; Fermi, Beatrice; Bosio, Maria Cristina

    2014-01-01

    By influencing the number of RNA molecules repeatedly synthesized from the same gene, the control of transcription reinitiation has the potential to shape the transcriptome. Transcription reinitiation mechanisms have been mainly addressed in vitro, through approaches based on both crude and reconstituted systems. These studies support the notion that transcription reinitiation and its regulation rely on dedicated networks of molecular interactions within transcription machineries. At the same time, comparison with in vivo transcription rates suggests that additional mechanisms, factors and conditions must exist in the nucleus, whose biochemical elucidation is a fascinating challenge for future in vitro transcription studies.

  13. Technical Training: Technical Training Seminar

    CERN Multimedia

    2004-01-01

    Tuesday 30 March TECHNICAL TRAINING SEMINAR From 9:00 to 12:00 and from 13:00 to 16:00 hrs - Council Chamber, Salle B, Salle des Pas Perdus National Instruments (NI) on Tour 2004 Claudia Jüngel, Evrem Yarkin, Joel Clerc, Hervé Baour / NATIONAL INSTRUMENTS The special event NI on Tour 2004, run in Germany, Austria and Switzerland, will be at CERN on March 30. Technical seminars and free introductory courses will be offered all day long in the Council Chamber, Salle B, and Salle des Pas Perdus (buildings 61 and 503). Technical conferences: 09:00 - 12:00 Data acquisition systems on PCs. Industrial measurement and control techniques. 13:00 - 16:00 Advanced LabVIEW software and PXI instrumentation. Measuring instruments and system components for teststand automation. Introductory courses: 09:00 - 12:00 DIAdem: Data analysis and presentation 13:00 - 16:00 Data acquisition with LabVIEW Language: English and French Free special seminar. Registration is recommended with National Instruments Switzerland (please sp...

  14. Technical Training: Technical Training Seminar

    CERN Multimedia

    2004-01-01

    Tuesday 30 March TECHNICAL TRAINING SEMINAR From 9:00 to 12:00 and from 13:00 to 16:00 hrs - Council Chamber, Salle B, Salle des Pas Perdus National Instruments (NI) on Tour 2004 Claudia Jüngel, Evrem Yarkin, Joel Clerc, Hervé Baour / NATIONAL INSTRUMENTS The special event NI on Tour 2004, run in Germany, Austria and Switzerland, will be at CERN on March 30. Technical seminars and free introductory courses will be offered all day long in the Council Chamber, Salle B, and Salle des Pas Perdus (buildings 61 and 503). Technical conferences: 09:00 - 12:00 Data acquisition systems on PCs. Industrial measurement and control techniques. 13:00 - 16:00 Advanced LabVIEW software and PXI instrumentation. Measuring instruments and system components for teststand automation. Introductory courses: 09:00 - 12:00 DIAdem: Data analysis and presentation 13:00 - 16:00 Data acquisition with LabVIEW Language: English and French Free special seminar. Registration is recommended with National Instruments Swi...

  15. Exercise induces transient transcriptional activation of the PGC-1a gene in human skeletal muscle

    DEFF Research Database (Denmark)

    Pilegaard, Henriette; Saltin, Bengt; Neufer, P. Darrell

    2003-01-01

    Endurance exercise training induces mitochondrial biogenesis in skeletal muscle. The peroxisome proliferator activated receptor co-activator 1a (PGC-1a) has recently been identified as a nuclear factor critical for coordinating the activation of genes required for mitochondrial biogenesis in cell...... culture and rodent skeletal muscle. To determine whether PGC-1a transcription is regulated by acute exercise and exercise training in human skeletal muscle, seven male subjects performed 4 weeks of one-legged knee extensor exercise training. At the end of training, subjects completed 3 h of two......-legged knee extensor exercise. Biopsies were obtained from the vastus lateralis muscle of both the untrained and trained legs before exercise and after 0, 2, 6 and 24 h of recovery. Time to exhaustion (2 min maximum resistance), as well as hexokinase II (HKII), citrate synthase and 3-hydroxyacyl...

  16. Training in Inductive Reasoning.

    Science.gov (United States)

    Tomic, Welko

    This study investigated the effects of K. J. Klauer's (1989) inductive reasoning training program of teaching children. Effects of training and the range of transfer of the training were assessed. The subjects were 34 third-grade Dutch children of average ability, matched on age, sex, and IQ. Children from the training condition (N=17) received…

  17. Functionality of intergenic transcription: an evolutionary comparison.

    Directory of Open Access Journals (Sweden)

    Philipp Khaitovich

    2006-10-01

    Full Text Available Although a large proportion of human transcription occurs outside the boundaries of known genes, the functional significance of this transcription remains unknown. We have compared the expression patterns of known genes as well as intergenic transcripts within the ENCODE regions between humans and chimpanzees in brain, heart, testis, and lymphoblastoid cell lines. We find that intergenic transcripts show patterns of tissue-specific conservation of their expression, which are comparable to exonic transcripts of known genes. This suggests that intergenic transcripts are subject to functional constraints that restrict their rate of evolutionary change as well as putative positive selection to an extent comparable to that of classical protein-coding genes. In brain and testis, we find that part of this intergenic transcription is caused by widespread use of alternative promoters. Further, we find that about half of the expression differences between humans and chimpanzees are due to intergenic transcripts.

  18. Functionality of Intergenic Transcription: An Evolutionary Comparison

    Science.gov (United States)

    Visagie, Johann; Giger, Thomas; Joerchel, Sabrina; Petzold, Ekkehard; Green, Richard E; Lachmann, Michael; Pääbo, Svante

    2006-01-01

    Although a large proportion of human transcription occurs outside the boundaries of known genes, the functional significance of this transcription remains unknown. We have compared the expression patterns of known genes as well as intergenic transcripts within the ENCODE regions between humans and chimpanzees in brain, heart, testis, and lymphoblastoid cell lines. We find that intergenic transcripts show patterns of tissue-specific conservation of their expression, which are comparable to exonic transcripts of known genes. This suggests that intergenic transcripts are subject to functional constraints that restrict their rate of evolutionary change as well as putative positive selection to an extent comparable to that of classical protein-coding genes. In brain and testis, we find that part of this intergenic transcription is caused by widespread use of alternative promoters. Further, we find that about half of the expression differences between humans and chimpanzees are due to intergenic transcripts. PMID:17040132

  19. Sry is a transcriptional activator.

    Science.gov (United States)

    Dubin, R A; Ostrer, H

    1994-09-01

    The SRY gene functions as a genetic switch in gonadal ridge initiating testis determination. The mouse Sry and human SRY open reading frames (ORFs) share a conserved DNA-binding domain (the HMG-box) yet exhibit no additional homology outside this region. As judged by the accumulation of lacZ-SRY hybrid proteins in the nucleus, both the human and mouse SRY ORFs contain a nuclear localization signal. The mouse Sry HMG-box domain selectively binds the sequence NACAAT in vitro when challenged with a random pool of oligonucleotides and binds AACAAT with the highest affinity. When put under the control of a heterologous promotor, the mouse Sry gene activated transcription of a reporter gene containing multiple copies of the AACAAT binding site. Activation was likewise observed for a GAL4-responsive reporter gene, when the mouse Sry gene was linked to the DNA-binding domain of GAL4. Using this system, the activation function was mapped to a glutamine/histidine-rich domain. In addition, LexA-mouse Sry fusion genes activated a LexA-responsive reporter gene in yeast. In contrast, a GAL4-human SRY fusion gene did not cause transcriptional activation. These studies suggest that both the human and the mouse SRY ORFs encode nuclear, DNA-binding proteins and that the mouse Sry ORF can function as a transcriptional activator with separable DNA-binding and activator domains.

  20. Mutual interdependence of splicing and transcription elongation.

    Science.gov (United States)

    Brzyżek, Grzegorz; Świeżewski, Szymon

    2015-01-01

    Transcription and splicing are intrinsically linked, as splicing needs a pre-mRNA substrate to commence. The more nuanced view is that the rate of transcription contributes to splicing regulation. On the other hand there is accumulating evidence that splicing has an active role in controlling transcription elongation by DNA-dependent RNA polymerase II (RNAP II). We briefly review those mechanisms and propose a unifying model where splicing controls transcription elongation to provide an optimal timing for successive rounds of splicing.

  1. Training In Diversity Management

    Directory of Open Access Journals (Sweden)

    Sonja Treven

    2011-07-01

    Full Text Available The labor force all around the world is becoming increasingly diverse. Thus, organizations that can manage employee diversity effectively gain a competitive advantage. In such organizations diversity training is a necessity. Diversity training helps managers understand and value individual differences and develop strong diagnostic skills. The paper explores various approaches to training, like awareness-based and skill-based diversity training. A special attention to potential problems that may occur in the process of diversity training is given.

  2. A unified model for yeast transcript definition.

    Science.gov (United States)

    de Boer, Carl G; van Bakel, Harm; Tsui, Kyle; Li, Joyce; Morris, Quaid D; Nislow, Corey; Greenblatt, Jack F; Hughes, Timothy R

    2014-01-01

    Identifying genes in the genomic context is central to a cell's ability to interpret the genome. Yet, in general, the signals used to define eukaryotic genes are poorly described. Here, we derived simple classifiers that identify where transcription will initiate and terminate using nucleic acid sequence features detectable by the yeast cell, which we integrate into a Unified Model (UM) that models transcription as a whole. The cis-elements that denote where transcription initiates function primarily through nucleosome depletion, and, using a synthetic promoter system, we show that most of these elements are sufficient to initiate transcription in vivo. Hrp1 binding sites are the major characteristic of terminators; these binding sites are often clustered in terminator regions and can terminate transcription bidirectionally. The UM predicts global transcript structure by modeling transcription of the genome using a hidden Markov model whose emissions are the outputs of the initiation and termination classifiers. We validated the novel predictions of the UM with available RNA-seq data and tested it further by directly comparing the transcript structure predicted by the model to the transcription generated by the cell for synthetic DNA segments of random design. We show that the UM identifies transcription start sites more accurately than the initiation classifier alone, indicating that the relative arrangement of promoter and terminator elements influences their function. Our model presents a concrete description of how the cell defines transcript units, explains the existence of nongenic transcripts, and provides insight into genome evolution.

  3. The great repression: chromatin and cryptic transcription.

    Science.gov (United States)

    Hennig, Bianca P; Fischer, Tamás

    2013-01-01

    The eukaryotic chromatin structure is essential in correctly defining transcription units. Impairing this structure can activate cryptic promoters, and lead to the accumulation of aberrant RNA transcripts. Here we discuss critical pathways that are responsible for the repression of cryptic transcription and the maintenance of genome integrity.

  4. TAF7: traffic controller in transcription initiation.

    Science.gov (United States)

    Gegonne, Anne; Devaiah, Ballachanda N; Singer, Dinah S

    2013-01-01

    TAF7, a component of the TFIID complex, controls the first steps of transcription. It interacts with and regulates the enzymatic activities of transcription factors that regulate RNA polymerase II progression. Its diverse functions in transcription initiation are consistent with its essential role in cell proliferation.

  5. Interplay between DNA supercoiling and transcription elongation.

    Science.gov (United States)

    Ma, Jie; Wang, Michelle

    2014-01-01

    Transcription-coupled DNA supercoiling has been shown to be an important regulator of transcription that is broadly present in the cell. Here we review experimental work which shows that RNA polymerase is a powerful torsional motor that can alter DNA topology and structure, and DNA supercoiling in turn directly affects transcription elongation.

  6. 18 CFR 1b.12 - Transcripts.

    Science.gov (United States)

    2010-04-01

    ... 18 Conservation of Power and Water Resources 1 2010-04-01 2010-04-01 false Transcripts. 1b.12 Section 1b.12 Conservation of Power and Water Resources FEDERAL ENERGY REGULATORY COMMISSION, DEPARTMENT OF ENERGY GENERAL RULES RULES RELATING TO INVESTIGATIONS § 1b.12 Transcripts. Transcripts, if any,...

  7. Finger Movements in Transcription Typing

    Science.gov (United States)

    1980-05-07

    Dr. Alfred F. Smode Office of Nvel Research Dr. Richard Gibson Training Aalysis & Rvalustion Group Arlington, VA 22217Dr. ~chnd Clson(TANG) Bureau of...Personnel PAD Center 1 Dr. Lloyd Hitchcock San Diego, CA 92152 Human Factors lagineerig 1 Roger W. loinSto, bh.D Division (6022) Code L52 I Technical Dirlctor

  8. A Grounded Theory Study of Supervision of Preservice Consultation Training

    Science.gov (United States)

    Newman, Daniel S.

    2012-01-01

    The purpose of this study was to explore a university-based supervision process for consultants-in-training (CITs) engaged in a preservice level consultation course with applied practicum experience. The study was approached from a constructivist worldview using a grounded theory methodology. Data consisted of supervision session transcripts,…

  9. Transcriptional and post-transcriptional regulation of a NAC1 transcription factor in Medicago truncatula roots.

    Science.gov (United States)

    D'haeseleer, Katrien; Den Herder, Griet; Laffont, Carole; Plet, Julie; Mortier, Virginie; Lelandais-Brière, Christine; De Bodt, Stefanie; De Keyser, Annick; Crespi, Martin; Holsters, Marcelle; Frugier, Florian; Goormachtig, Sofie

    2011-08-01

    • Legume roots develop two types of lateral organs, lateral roots and nodules. Nodules develop as a result of a symbiotic interaction with rhizobia and provide a niche for the bacteria to fix atmospheric nitrogen for the plant. • The Arabidopsis NAC1 transcription factor is involved in lateral root formation, and is regulated post-transcriptionally by miRNA164 and by SINAT5-dependent ubiquitination. We analyzed in Medicago truncatula the role of the closest NAC1 homolog in lateral root formation and in nodulation. • MtNAC1 shows a different expression pattern in response to auxin than its Arabidopsis homolog and no changes in lateral root number or nodulation were observed in plants affected in MtNAC1 expression. In addition, no interaction was found with SINA E3 ligases, suggesting that post-translational regulation of MtNAC1 does not occur in M. truncatula. Similar to what was found in Arabidopsis, a conserved miR164 target site was retrieved in MtNAC1, which reduced protein accumulation of a GFP-miR164 sensor. Furthermore, miR164 and MtNAC1 show an overlapping expression pattern in symbiotic nodules, and overexpression of this miRNA led to a reduction in nodule number. • This work suggests that regulatory pathways controlling a conserved transcription factor are complex and divergent between M. truncatula and Arabidopsis.

  10. Contributions of in vitro transcription to the understanding of human RNA polymerase III transcription.

    Science.gov (United States)

    Dumay-Odelot, Hélène; Durrieu-Gaillard, Stéphanie; El Ayoubi, Leyla; Parrot, Camila; Teichmann, Martin

    2014-01-01

    Human RNA polymerase III transcribes small untranslated RNAs that contribute to the regulation of essential cellular processes, including transcription, RNA processing and translation. Analysis of this transcription system by in vitro transcription techniques has largely contributed to the discovery of its transcription factors and to the understanding of the regulation of human RNA polymerase III transcription. Here we review some of the key steps that led to the identification of transcription factors and to the definition of minimal promoter sequences for human RNA polymerase III transcription.

  11. Transcription factories: genetic programming in three dimensions.

    Science.gov (United States)

    Edelman, Lucas Brandon; Fraser, Peter

    2012-04-01

    Among the most intensively studied systems in molecular biology is the eukaryotic transcriptional apparatus, which expresses genes in a regulated manner across hundreds of different cell types. Several studies over the past few years have added weight to the concept that transcription takes place within discrete 'transcription factories' assembled inside the cell nucleus. These studies apply innovative technical approaches to gain insights into the molecular constituents, dynamical behaviour and organizational regulators of transcription factories, providing exciting insights into the spatial dimension of transcriptional control.

  12. Transcriptional Regulation of Heart Development in Zebrafish

    Science.gov (United States)

    Lu, Fei; Langenbacher, Adam D.; Chen, Jau-Nian

    2016-01-01

    Cardiac transcription factors orchestrate the complex cellular and molecular events required to produce a functioning heart. Misregulation of the cardiac transcription program leads to embryonic developmental defects and is associated with human congenital heart diseases. Recent studies have expanded our understanding of the regulation of cardiac gene expression at an additional layer, involving the coordination of epigenetic and transcriptional regulators. In this review, we highlight and discuss discoveries made possible by the genetic and embryological tools available in the zebrafish model organism, with a focus on the novel functions of cardiac transcription factors and epigenetic and transcriptional regulatory proteins during cardiogenesis. PMID:27148546

  13. Contribution of transcription to animal early development.

    Science.gov (United States)

    Wang, Jianbin; Davis, Richard E

    2014-01-01

    In mature gametes and during the oocyte-to-embryo transition, transcription is generally silenced and gene expression is post-transcriptionally regulated. However, we recently discovered that major transcription can occur immediately after fertilization, prior to pronuclear fusion, and in the first cell division of the oocyte-to-embryo transition in the nematode Ascaris suum. We postulate that the balance between transcriptional and post-transcriptional regulation during the oocyte-to-embryo transition may largely be determined by cell cycle length and thus the time available for the genome to be transcribed.

  14. Transcriptional networks in leaf senescence.

    Science.gov (United States)

    Schippers, Jos H M

    2015-10-01

    Plant senescence is a natural phenomenon known for the appearance of beautiful autumn colors and the ripening of cereals in the field. Senescence is a controlled process that plants utilize to remobilize nutrients from source leaves to developing tissues. While during the past decades, molecular components underlying the onset of senescence have been intensively studied, knowledge remains scarce on the age-dependent mechanisms that control the onset of senescence. Recent advances have uncovered transcriptional networks regulating the competence to senesce. Here, gene regulatory networks acting as internal timing mechanisms for the onset of senescence are highlighted, illustrating that early and late leaf developmental phases are highly connected.

  15. The transcriptional regulation of pluripotency

    Institute of Scientific and Technical Information of China (English)

    Jia-Chi Yeo; Huck-Hui Ng

    2013-01-01

    The defining features of embryonic stem cells (ESCs) are their self-renewing and pluripotent capacities.Indeed,the ability to give rise into all cell types within the organism not only allows ESCs to function as an ideal in vitro tool to study embryonic development,but also offers great therapeutic potential within the field of regenerative medicine.However,it is also this same remarkable developmental plasticity that makes the efficient control of ESC differentiation into the desired cell type very difficult.Therefore,in order to harness ESCs for clinical applications,a detailed understanding of the molecular and cellular mechanisms controlling ESC pluripotency and lineage commitment is necessary.In this respect,through a variety of transcriptomic approaches,ESC pluripotency has been found to be regulated by a system of ESC-associated transcription factors; and the external signalling environment also acts as a key factor in modulating the ESC transcriptome.Here in this review,we summarize our current understanding of the transcriptional regulatory network in ESCs,discuss how the control of various signalling pathways could influence pluripotency,and provide a future outlook of ESC research.

  16. Technical training - places available

    CERN Multimedia

    2013-01-01

    If you would like more information on a course, or have any other inquiry/suggestions, please contact Technical.Training@cern.ch. Eva Stern and Elise Romero, Technical Training Administration (Tel: 74924)

  17. Technical training: places available

    CERN Multimedia

    2014-01-01

    If you would like more information on a course, or have any other inquiry/suggestions, please contact Technical.Training@cern.ch. Eva Stern and Marie Lahchimi, Technical Training Administration (Tel: 74924)

  18. Technical training: places available

    CERN Multimedia

    2013-01-01

    If you would like more information on a course, or for any other inquiry/suggestions, please contact Technical.Training@cern.ch. Eva Stern and Elise Romero, Technical Training Administration (Tel: 74924)

  19. Technical training: places available

    CERN Multimedia

    2013-01-01

    If you would like more information on a course, or for any other inquiry/suggestions, please contact Technical.Training@cern.ch. Eva Stern and Elise Romero, Technical Training Administration (Tél : 74924)  

  20. Technical training: places available

    CERN Multimedia

    2014-01-01

    If you would like more information on a course, or have any other inquiry/suggestions, please contact Technical.Training@cern.ch. Eva Stern and Elise Romero, Technical Training Administration (Tel: 74924)

  1. Importance of Gait Training

    Science.gov (United States)

    ... amputation exercises done under the supervision of a physical therapist, the initial training is provided by the prosthetist ... this stage, it is best to involve a physical therapist for regular gait training sessions. Once it is ...

  2. Moldova - Value Chain Training

    Data.gov (United States)

    Millennium Challenge Corporation — The evaluation of the GHS value chain training subactivity wwas designed to measure the extent, if any, to which the training activities improved the productivity...

  3. DBD: a transcription factor prediction database.

    Science.gov (United States)

    Kummerfeld, Sarah K; Teichmann, Sarah A

    2006-01-01

    Regulation of gene expression influences almost all biological processes in an organism; sequence-specific DNA-binding transcription factors are critical to this control. For most genomes, the repertoire of transcription factors is only partially known. Hitherto transcription factor identification has been largely based on genome annotation pipelines that use pairwise sequence comparisons, which detect only those factors similar to known genes, or on functional classification schemes that amalgamate many types of proteins into the category of 'transcription factor'. Using a novel transcription factor identification method, the DBD transcription factor database fills this void, providing genome-wide transcription factor predictions for organisms from across the tree of life. The prediction method behind DBD identifies sequence-specific DNA-binding transcription factors through homology using profile hidden Markov models (HMMs) of domains. Thus, it is limited to factors that are homologus to those HMMs. The collection of HMMs is taken from two existing databases (Pfam and SUPERFAMILY), and is limited to models that exclusively detect transcription factors that specifically recognize DNA sequences. It does not include basal transcription factors or chromatin-associated proteins, for instance. Based on comparison with experimentally verified annotation, the prediction procedure is between 95% and 99% accurate. Between one quarter and one-half of our genome-wide predicted transcription factors represent previously uncharacterized proteins. The DBD (www.transcriptionfactor.org) consists of predicted transcription factor repertoires for 150 completely sequenced genomes, their domain assignments and the hand curated list of DNA-binding domain HMMs. Users can browse, search or download the predictions by genome, domain family or sequence identifier, view families of transcription factors based on domain architecture and receive predictions for a protein sequence.

  4. Criticality safety training

    Energy Technology Data Exchange (ETDEWEB)

    Woodruff, S.K. [Los Alamos National Lab., NM (United States)

    1997-06-01

    Criticality safety training is an important element of the Plutonium Facility safety program at Los Alamos National Laboratory. Training consists of student self-study handbooks and hands-on performance-based training in a mock-up laboratory containing gloveboxes, trolley conveyor system, and self-monitoring instruments. A 10-minute video tape and lecture was presented to describe how training in this area is conducted.

  5. Mitotic Transcriptional Activation: Clearance of Actively Engaged Pol II via Transcriptional Elongation Control in Mitosis.

    Science.gov (United States)

    Liang, Kaiwei; Woodfin, Ashley R; Slaughter, Brian D; Unruh, Jay R; Box, Andrew C; Rickels, Ryan A; Gao, Xin; Haug, Jeffrey S; Jaspersen, Sue L; Shilatifard, Ali

    2015-11-05

    Although it is established that some general transcription factors are inactivated at mitosis, many details of mitotic transcription inhibition (MTI) and its underlying mechanisms are largely unknown. We have identified mitotic transcriptional activation (MTA) as a key regulatory step to control transcription in mitosis for genes with transcriptionally engaged RNA polymerase II (Pol II) to activate and transcribe until the end of the gene to clear Pol II from mitotic chromatin, followed by global impairment of transcription reinitiation through MTI. Global nascent RNA sequencing and RNA fluorescence in situ hybridization demonstrate the existence of transcriptionally engaged Pol II in early mitosis. Both genetic and chemical inhibition of P-TEFb in mitosis lead to delays in the progression of cell division. Together, our study reveals a mechanism for MTA and MTI whereby transcriptionally engaged Pol II can progress into productive elongation and finish transcription to allow proper cellular division.

  6. District nurse training

    OpenAIRE

    Elliott, Arnold; Freeling, Paul; Owen, John

    1980-01-01

    Training for district nursing is being reviewed. By 1981 district nurses will have a new administrative structure, a new curriculum, and a new examination. Training for nursing, like that for general practice, is to become mandatory. The history of the development of district nurse training is briefly described.

  7. Hard-Nosed Training

    Science.gov (United States)

    Willard, Norman, Jr.

    1973-01-01

    Training supervisor at Citibank accepts role as manager and considers aim to increase corporate earnings through cost-effective training. Based on an address delivered at the First Annual Training in Business and Industry Conference in New York, New York. (DS)

  8. Strength Training for Girls.

    Science.gov (United States)

    Connaughton, Daniel; Connaughton, Angela; Poor, Linda

    2001-01-01

    Strength training can be fun, safe, and appropriate for young girls and women and is an important component of any fitness program when combined with appropriate cardiovascular and flexibility activities. Concerns and misconceptions regarding girls' strength training are discussed, presenting general principles of strength training for children…

  9. Training and Learning

    Science.gov (United States)

    Luntley, Michael

    2008-01-01

    Some philosophers of education think that there is a pedagogically informative concept of training that can be gleaned from Wittgenstein's later writings: training as initiation into a form of life. Stickney, in "Training and Mastery of Techniques in Wittgenstein's Later Philosophy: A response to Michael Luntley" takes me to task for ignoring this…

  10. Employment and Training Programs.

    Science.gov (United States)

    Minnesota State Office of the Legislative Auditor, St. Paul. Program Evaluation Div.

    This report examines the effectiveness of employment and training programs in Minnesota and discusses the impact of the 1985 Jobs Bill state legislation. Chapter 1 provides an introduction to Minnesota's programs and to studies of employment and training programs conducted nationwide. Chapter 2 studies the use of Job Training Partnership Act funds…

  11. Catching transcriptional regulation by thermostatistical modeling

    Science.gov (United States)

    Frank, Till D.; Cheong, Alex; Okada-Hatakeyama, Mariko; Kholodenko, Boris N.

    2012-08-01

    Gene expression is frequently regulated by multiple transcription factors (TFs). Thermostatistical methods allow for a quantitative description of interactions between TFs, RNA polymerase and DNA, and their impact on the transcription rates. We illustrate three different scales of the thermostatistical approach: the microscale of TF molecules, the mesoscale of promoter energy levels and the macroscale of transcriptionally active and inactive cells in a cell population. We demonstrate versatility of combinatorial transcriptional activation by exemplifying logic functions, such as AND and OR gates. We discuss a metric for cell-to-cell transcriptional activation variability known as Fermi entropy. Suitability of thermostatistical modeling is illustrated by describing the experimental data on transcriptional induction of NFκB and the c-Fos protein.

  12. Systematic genetic analysis of transcription factors to map the fission yeast transcription-regulatory network.

    Science.gov (United States)

    Chua, Gordon

    2013-12-01

    Mapping transcriptional-regulatory networks requires the identification of target genes, binding specificities and signalling pathways of transcription factors. However, the characterization of each transcription factor sufficiently for deciphering such networks remains laborious. The recent availability of overexpression and deletion strains for almost all of the transcription factor genes in the fission yeast Schizosaccharomyces pombe provides a valuable resource to better investigate transcription factors using systematic genetics. In the present paper, I review and discuss the utility of these strain collections combined with transcriptome profiling and genome-wide chromatin immunoprecipitation to identify the target genes of transcription factors.

  13. Transcription in Archaea: in vitro transcription assays for mjRNAP.

    Science.gov (United States)

    Smollett, Katherine; Blombach, Fabian; Werner, Finn

    2015-01-01

    The fully recombinant Methanocaldococcus jannaschii RNA polymerase allows for a detailed dissection of the different stages of the transcription. In the previous chapter, we discussed how to purify the different components of the M. jannaschii transcription system, the RNA polymerase subunits, and general transcription factors and how to assemble a functional M. jannaschii enzyme. Standard in vitro transcription assays can be used to examine the different stages of transcription. In this chapter, we describe how some of these assays have been optimized for M. jannaschii RNA polymerase, which transcribes at much higher temperatures than many other transcription complexes.

  14. Balanced Branching in Transcription Termination

    CERN Document Server

    Harrington, K J; Liang, S

    2000-01-01

    The theory of stochastic transcription termination based on free-energy competition requires two or more reaction rates to be delicately balanced over a wide range of physical conditions. A large body of work on glasses and large molecules suggests that this should be impossible in such a large system in the absence of a new organizing principle of matter. We review the experimental literature of termination and find no evidence for such a principle but many troubling inconsistencies, most notably anomalous memory effects. These suggest that termination has a deterministic component and may conceivably be not stochastic at all. We find that a key experiment by Wilson and von Hippel allegedly refuting deterministic termination was an incorrectly analyzed regulatory effect of Mg2+ binding.

  15. Transcriptional Regulation and Macrophage Differentiation.

    Science.gov (United States)

    Hume, David A; Summers, Kim M; Rehli, Michael

    2016-06-01

    Monocytes and macrophages are professional phagocytes that occupy specific niches in every tissue of the body. Their survival, proliferation, and differentiation are controlled by signals from the macrophage colony-stimulating factor receptor (CSF-1R) and its two ligands, CSF-1 and interleukin-34. In this review, we address the developmental and transcriptional relationships between hematopoietic progenitor cells, blood monocytes, and tissue macrophages as well as the distinctions from dendritic cells. A huge repertoire of receptors allows monocytes, tissue-resident macrophages, or pathology-associated macrophages to adapt to specific microenvironments. These processes create a broad spectrum of macrophages with different functions and individual effector capacities. The production of large transcriptomic data sets in mouse, human, and other species provides new insights into the mechanisms that underlie macrophage functional plasticity.

  16. Control and signal processing by transcriptional interference

    OpenAIRE

    2009-01-01

    A transcriptional activator can suppress gene expression by interfering with transcription initiated by another activator. Transcriptional interference has been increasingly recognized as a regulatory mechanism of gene expression. The signals received by the two antagonistically acting activators are combined by the polymerase trafficking along the DNA. We have designed a dual-control genetic system in yeast to explore this antagonism systematically. Antagonism by an upstream activator bears ...

  17. A New Vaccinia Virus Intermediate Transcription Factor

    OpenAIRE

    Sanz, Patrick; Moss, Bernard

    1998-01-01

    Transcription of the vaccinia virus genome is mediated by a virus-encoded multisubunit DNA-dependent RNA polymerase in conjunction with early-, intermediate-, and late-stage-specific factors. Previous studies indicated that two virus-encoded proteins (capping enzyme and VITF-1) and one unidentified cellular protein (VITF-2) are required for specific transcription of an intermediate promoter template in vitro. We have now extensively purified an additional virus-induced intermediate transcript...

  18. Strength Training and Your Child

    Science.gov (United States)

    ... Feeding Your 1- to 2-Year-Old Strength Training and Your Child KidsHealth > For Parents > Strength Training ... prevent injuries and speed up recovery. About Strength Training Strength training is the practice of using free ...

  19. Radiological worker training

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1998-10-01

    This Handbook describes an implementation process for core training as recommended in Implementation Guide G441.12, Radiation Safety Training, and as outlined in the DOE Radiological Control Standard (RCS). The Handbook is meant to assist those individuals within the Department of Energy, Managing and Operating contractors, and Managing and Integrating contractors identified as having responsibility for implementing core training recommended by the RCS. This training is intended for radiological workers to assist in meeting their job-specific training requirements of 10 CFR 835. While this Handbook addresses many requirements of 10 CFR 835 Subpart J, it must be supplemented with facility-specific information to achieve full compliance.

  20. Workmanship Training Industry

    Science.gov (United States)

    Patterson, Ashley; Sikes, Larry; Corbin, Cheryl; Rucka, Rebecca

    2015-01-01

    Special processes require special skills, knowledge and experienced application. For over 15 years, the NASA Johnson Space Center's Receiving, Inspection and Test Facility (RITF) has provided Workmanship Standards compliance training, issuing more than 500 to 800 training completion certificates annually. It is critical that technicians and inspectors are trained and that they maintain their proficiency to implement the applicable standards and specifications. Training services include 'hands-on' training to engineers, technicians, and inspectors in the areas of electrostatic discharge (ESD), soldering, fiber optics, lithium battery handling, torque and wire safety, and wire wrapping.

  1. Promoter proximal polyadenylation sites reduce transcription activity

    DEFF Research Database (Denmark)

    Andersen, Pia Kjølhede; Lykke-Andersen, Søren; Jensen, Torben Heick

    2012-01-01

    on transcription requires promoter proximity, as demonstrated using artificial constructs and supported by a genome-wide data set. Importantly, transcription down-regulation can be recapitulated in a gene context devoid of splice sites by placing a functional bona fide pA site/transcription terminator within ∼500...... base pairs of the promoter. In contrast, promoter-proximal positioning of a pA site-independent histone gene terminator supports high transcription levels. We propose that optimal communication between a pA site-dependent gene terminator and its promoter critically depends on gene length and that short...

  2. Heritable change caused by transient transcription errors.

    Directory of Open Access Journals (Sweden)

    Alasdair J E Gordon

    2013-06-01

    Full Text Available Transmission of cellular identity relies on the faithful transfer of information from the mother to the daughter cell. This process includes accurate replication of the DNA, but also the correct propagation of regulatory programs responsible for cellular identity. Errors in DNA replication (mutations and protein conformation (prions can trigger stable phenotypic changes and cause human disease, yet the ability of transient transcriptional errors to produce heritable phenotypic change ('epimutations' remains an open question. Here, we demonstrate that transcriptional errors made specifically in the mRNA encoding a transcription factor can promote heritable phenotypic change by reprogramming a transcriptional network, without altering DNA. We have harnessed the classical bistable switch in the lac operon, a memory-module, to capture the consequences of transient transcription errors in living Escherichia coli cells. We engineered an error-prone transcription sequence (A9 run in the gene encoding the lac repressor and show that this 'slippery' sequence directly increases epigenetic switching, not mutation in the cell population. Therefore, one altered transcript within a multi-generational series of many error-free transcripts can cause long-term phenotypic consequences. Thus, like DNA mutations, transcriptional epimutations can instigate heritable changes that increase phenotypic diversity, which drives both evolution and disease.

  3. Swinger RNAs with sharp switches between regular transcription and transcription systematically exchanging ribonucleotides: Case studies.

    Science.gov (United States)

    Seligmann, Hervé

    2015-09-01

    During RNA transcription, DNA nucleotides A,C,G, T are usually matched by ribonucleotides A, C, G and U. However occasionally, this rule does not apply: transcript-DNA homologies are detectable only assuming systematic exchanges between ribonucleotides. Nine symmetric (X ↔ Y, e.g. A ↔ C) and fourteen asymmetric (X ↔ Y ↔ Z, e.g. A ↔ C ↔ G) exchanges exist, called swinger transcriptions. Putatively, polymerases occasionally stabilize in unspecified swinger conformations, possibly similar to transient conformations causing punctual misinsertions. This predicts chimeric transcripts, part regular, part swinger-transformed, reflecting polymerases switching to swinger polymerization conformation(s). Four chimeric Genbank transcripts (three from human mitochondrion and one murine cytosolic) are described here: (a) the 5' and 3' extremities reflect regular polymerization, the intervening sequence exchanges systematically between ribonucleotides (swinger rule G ↔ U, transcript (1), with sharp switches between regular and swinger sequences; (b) the 5' half is 'normal', the 3' half systematically exchanges ribonucleotides (swinger rule C ↔ G, transcript (2), with an intercalated sequence lacking homology; (c) the 3' extremity fits A ↔ G exchanges (10% of transcript length), the 5' half follows regular transcription; the intervening region seems a mix of regular and A ↔ G transcriptions (transcript 3); (d) murine cytosolic transcript 4 switches to A ↔ U + C ↔ G, and is fused with A ↔ U + C ↔ G swinger transformed precursor rRNA. In (c), each concomitant transcript 5' and 3' extremities match opposite genome strands. Transcripts 3 and 4 combine transcript fusions with partial swinger transcriptions. Occasional (usually sharp) switches between regular and swinger transcriptions reveal greater coding potential than detected until now, suggest stable polymerase swinger conformations.

  4. [The robotic surgeon training].

    Science.gov (United States)

    Crestani, Alessandro; Rossanese, Marta; Abbinante, Maria; Calandriello, Mattia; Kungulli, Afrovita; Giannarini, Gianluca; Ficarra, Vincenzo

    2015-10-01

    The widespread robotic surgery in the world highlighted the relevance of the training programs for young urologists and residents. In the last years, urologic societies and some independent robotic surgeons strongly worked to standardize some general and specific training modules. Theoretical and practical sections of robotic training programs have been recently specified. The role of simulators, dry and wet laboratories, bedside assistance, and modular (step-by-step) training at console represent the most relevant elements of robotic surgeon training. Ideally, these didactic tools should be available in modern training centers. The development of structured robotic training programs should be considered as one of the priorities that the urologic community must take into account in the near future.

  5. Academic Training: Academic Training Lectures-Questionnaire

    CERN Multimedia

    2004-01-01

    ACADEMIC TRAINING Françoise Benz tel. 73127 academic.training@cern.ch SUGGEST AND WIN! Its time to plan the 2004-2005 lecture series. From today until March 19 you have the chance to give your contribution to planning for next year's Academic Training Lecture Series. At the web site: http://cern.ch/Academic.Training/questionnaire you will find questionnaires proposing topics in high energy physics, applied physics and science and society. Answering the questionnaire will help ensure that the selected topics are as close as possible to your interests. In particular requests and comments from students will be much appreciated. To encourage your contribution, the AT Committee will reward one lucky winner with a small prize, a 50 CHF coupon for a book purchase at the CERN bookshop.

  6. Carbohydrate availability and exercise training adaptation: too much of a good thing?

    Science.gov (United States)

    Bartlett, Jonathan D; Hawley, John A; Morton, James P

    2015-01-01

    Traditional nutritional approaches to endurance training have typically promoted high carbohydrate (CHO) availability before, during and after training sessions to ensure adequate muscle substrate to meet the demands of high daily training intensities and volumes. However, during the past decade, data from our laboratories and others have demonstrated that deliberately training in conditions of reduced CHO availability can promote training-induced adaptations of human skeletal muscle (i.e. increased maximal mitochondrial enzyme activities and/or mitochondrial content, increased rates of lipid oxidation and, in some instances, improved exercise capacity). Such data have led to the concept of 'training low, but competing high' whereby selected training sessions are completed in conditions of reduced CHO availability (so as to promote training adaptation), but CHO reserves are restored immediately prior to an important competition. The augmented training response observed with training-low strategies is likely regulated by enhanced activation of key cell signalling kinases (e.g. AMPK, p38MAPK), transcription factors (e.g. p53, PPARδ) and transcriptional co-activators (e.g. PGC-1α), such that a co-ordinated up-regulation of both the nuclear and mitochondrial genomes occurs. Although the optimal practical strategies to train low are not currently known, consuming additional caffeine, protein, and practising CHO mouth-rinsing before and/or during training may help to rescue the reduced training intensities that typically occur when 'training low', in addition to preventing protein breakdown and maintaining optimal immune function. Finally, athletes should practise 'train-low' workouts in conjunction with sessions undertaken with normal or high CHO availability so that their capacity to oxidise CHO is not blunted on race day.

  7. Transcriptional interference by RNA polymerase pausing and dislodgement of transcription factors.

    Science.gov (United States)

    Palmer, Adam C; Egan, J Barry; Shearwin, Keith E

    2011-01-01

    Transcriptional interference is the in cis suppression of one transcriptional process by another. Mathematical modeling shows that promoter occlusion by elongating RNA polymerases cannot produce strong interference. Interference may instead be generated by (1) dislodgement of slow-to-assemble pre-initiation complexes and transcription factors and (2) prolonged occlusion by paused RNA polymerases.

  8. U.S. Army Research Laboratory (ARL) Corporate Dari Document Transcription and Translation Guidelines

    Science.gov (United States)

    2012-10-01

    text file format. 15. SUBJECT TERMS Transcription, Translation, guidelines, ground truth, Optical character recognition, OCR, Machine Translation, MT...foreign language into a target language in order to train, test, and evaluate optical character recognition (OCR) and machine translation (MT) embedded...the final word in this sequence). 5. Spacing: When printed words give the illusion of having a space in between letters in the word due to

  9. Transcription of Byzantine Chant - Problems, Possibilities, Formats

    DEFF Research Database (Denmark)

    Troelsgård, Christian

    2007-01-01

    Discusses the problems and possibilities for transsription of Byzantine chant on the basis of medieval musical manuscripts. A relatively 'neutral' style of transcription is suggested for musicological purposes.......Discusses the problems and possibilities for transsription of Byzantine chant on the basis of medieval musical manuscripts. A relatively 'neutral' style of transcription is suggested for musicological purposes....

  10. Transcription and the aspect ratio of DNA

    DEFF Research Database (Denmark)

    Olsen, Kasper Wibeck; Bohr, Jakob

    2013-01-01

    analysis of transcription. It is shown that under certain reasonable assumptions transcription is only possible if the aspect ratio is in the regime corresponding to further twisting. We find this constraint to be in agreement with long-established crystallographic studies of DNA....

  11. RNA polymerase II collision interrupts convergent transcription

    DEFF Research Database (Denmark)

    Hobson, David J; Wei, Wu; Steinmetz, Lars M

    2012-01-01

    Antisense noncoding transcripts, genes-within-genes, and convergent gene pairs are prevalent among eukaryotes. The existence of such transcription units raises the question of what happens when RNA polymerase II (RNAPII) molecules collide head-to-head. Here we use a combination of biochemical...

  12. Preliminary research on the carbonyl compounds under of sea-land breeze at the west coast of the Pearl River Estuary%珠江口西岸冬季海陆风背景下羰基化合物的初步研究

    Institute of Scientific and Technical Information of China (English)

    庄延娟; 王伯光; 刘灿

    2011-01-01

    A field observation was continuously conducted at Xinken, Guangzhou, China, using DNPH-HPLC/UV method to measure the composition and the concentration of volatile carbonyl compounds under sea-land breeze in the winter period from December 7th-9th, 2008. The distribution of carbonyl and their sources were studied. The results show that 17 carbonyl species were detected with a concentration range from 7.78×10 9 to 31.78×10-9. The concentrations of most carbonyls were higher on the ground than on the roof. Acetone was the most abundant carbonyl species at Xinken,followed by formaldehyde and acetaldehyde. The three compounds accounted for more than 70% of all measured carbonyls. Two different variation trends of the carbonyl compounds at Xinken were found. The first one was on December 8th suggesting the long-distance transport of urban pollutants while the other one was on December 7th and December 9th showing the influence of sea-land breeze. Based on the ratios among formaldehyde, acetaldehyde and propanal and the component correlation analysis, it indicates that the carbonyl compounds were mainly impacted by the urban anthropogenic pollution sources which had a close relationship with the long-distance transport and sea-land breeze influence. Secondary products from atmospheric photochemical reactions and local emission from vegetation were also the important contributors.%于2008年12月7~9日对珠江三角洲新垦的大气环境进行连续加强观测,采用DNPH-HPL洲分析方法测量了冬季海陆风条件下大气挥发性羰基化合物的组成及浓度水平,研究了污染特征和来源.结果表明,新垦大气中共检测到17种挥发性羰基化合物,浓度变化范围为7.78×10-9~31.78×10-9,大多数物质地面浓度高于楼顶.其中浓度最高的污染物是丙酮,其次是甲醛和乙醛,三者占总羰基化合物浓度的比例高于70%.大气挥发性羰基化合物的浓度分布呈现出以12月8日为代表的受城区污

  13. The Journey of a Transcription Factor

    DEFF Research Database (Denmark)

    Pireyre, Marie

    Plants have developed astonishing networks regulating their metabolism to adapt to their environment. The complexity of these networks is illustrated by the expansion of families of regulators such as transcription factors in the plant kingdom. Transcription factors specifically impact...... transcriptional networks by integrating exogenous and endogenous stimuli and regulating gene expression accordingly. Regulation of transcription factors and their activation is thus highly important to modulate the transcriptional programs and increase fitness of the plant in a given environment. Plant metabolism...... is regulated to allocate resources to growth and/or defense at different time points. Among plant chemical defenses are the amino acid-derived glucosinolates (GLS). Their absolute and relative accumulation is tightly regulated at basal level, but also in response to e.g. pathogen attack and hormone stimuli...

  14. Combinatorial Regulation in Yeast Transcription Networks

    Science.gov (United States)

    Li, Hao

    2006-03-01

    Yeast has evolved a complex network to regulate its transcriptional program in response to changes in environment. It is quite common that in response to an external stimulus, several transcription factors will be activated and they work in combinations to control different subsets of genes in the genome. We are interested in how the promoters of genes are designed to integrate signals from multiple transcription factors and what are the functional and evolutionary constraints. To answer how, we have developed a number of computational algorithms to systematically map the binding sites and target genes of transcription factors using sequence and gene expression data. To analyze the functional constraints, we have employed mechanistic models to study the dynamic behavior of genes regulated by multiple factors. We have also developed methods to trace the evolution of transcriptional networks via comparative analysis of multiple species.

  15. Transcription Factor Networks in Drosophila melanogaster

    Directory of Open Access Journals (Sweden)

    David Y. Rhee

    2014-09-01

    Full Text Available Specific cellular fates and functions depend on differential gene expression, which occurs primarily at the transcriptional level and is controlled by complex regulatory networks of transcription factors (TFs. TFs act through combinatorial interactions with other TFs, cofactors, and chromatin-remodeling proteins. Here, we define protein-protein interactions using a coaffinity purification/mass spectrometry method and study 459 Drosophila melanogaster transcription-related factors, representing approximately half of the established catalog of TFs. We probe this network in vivo, demonstrating functional interactions for many interacting proteins, and test the predictive value of our data set. Building on these analyses, we combine regulatory network inference models with physical interactions to define an integrated network that connects combinatorial TF protein interactions to the transcriptional regulatory network of the cell. We use this integrated network as a tool to connect the functional network of genetic modifiers related to mastermind, a transcriptional cofactor of the Notch pathway.

  16. Histone variants in plant transcriptional regulation.

    Science.gov (United States)

    Jiang, Danhua; Berger, Frédéric

    2017-01-01

    Chromatin based organization of eukaryotic genome plays a profound role in regulating gene transcription. Nucleosomes form the basic subunits of chromatin by packaging DNA with histone proteins, impeding the access of DNA to transcription factors and RNA polymerases. Exchange of histone variants in nucleosomes alters the properties of nucleosomes and thus modulates DNA exposure during transcriptional regulation. Growing evidence indicates the important function of histone variants in programming transcription during developmental transitions and stress response. Here we review how histone variants and their deposition machineries regulate the nucleosome stability and dynamics, and discuss the link between histone variants and transcriptional regulation in plants. This article is part of a Special Issue entitled: Plant Gene Regulatory Mechanisms and Networks, edited by Dr. Erich Grotewold and Dr. Nathan Springer.

  17. Transcriptional factors, Mafs and their biological roles

    Institute of Scientific and Technical Information of China (English)

    Mariko Tsuchiya; Ryoichi Misaka; Kosaku Nitta; Ken Tsuchiya

    2015-01-01

    The Maf family of transcription factors is characterizedby a typical bZip structure; these transcription factorsact as important regulators of the development anddifferentiation of many organs and tissues, includingthe kidney. The Maf family consists of two subgroupsthat are characterized according to their structure largeMaf transcription factors and small Maf transcriptionfactors. The large Maf subgroup consists of fourproteins, designated as MAFA, MAFB, c-MAF and neuralretina-specific leucine zipper. In particular, MAFA is adistinct molecule that has been attracting the attentionof researchers because it acts as a strong transactivatorof insulin, suggesting that Maf transcription factors arelikely to be involved in systemic energy homeostasis. Inthis review, we focused on the regulation of glucose/energy balance by Maf transcription factors in variousorgans.

  18. Transcriptional Regulation of Plant Secondary Metabolism

    Institute of Scientific and Technical Information of China (English)

    Chang-Qing Yang; Xin Fang; Xiu-Ming Wu; Ying-Bo Mao; Ling-Jian Wang; Xiao-Ya Chen

    2012-01-01

    Plant secondary metabolites play critical roles in plant-environment interactions.They are synthesized in different organs or tissues at particular developmental stages,and in response to various environmental stimuli,both biotic and abiotic.Accordingly,corresponding genes are regulated at the transcriptional level by multiple transcription factors.Several families of transcription factors have been identified to participate in controlling the biosynthesis and accumulation of secondary metabolites.These regulators integrate internal (often developmental) and external signals,bind to corresponding cis-elements — which are often in the promoter regions — to activate or repress the expression of enzyme-coding genes,and some of them interact with other transcription factors to form a complex.In this review,we summarize recent research in these areas,with an emphasis on newly-identified transcription factors and their functions in metabolism regulation.

  19. Transcriptional profiling of epidermal differentiation.

    Science.gov (United States)

    Radoja, Nada; Gazel, Alix; Banno, Tomohiro; Yano, Shoichiro; Blumenberg, Miroslav

    2006-10-03

    In epidermal differentiation basal keratinocytes detach from the basement membrane, stop proliferating, and express a new set of structural proteins and enzymes, which results in an impermeable protein/lipid barrier that protects us. To define the transcriptional changes essential for this process, we purified large quantities of basal and suprabasal cells from human epidermis, using the expression of beta4 integrin as the discriminating factor. The expected expression differences in cytoskeletal, cell cycle, and adhesion genes confirmed the effective separation of the cell populations. Using DNA microarray chips, we comprehensively identify the differences in genes expressed in basal and differentiating layers of the epidermis, including the ECM components produced by the basal cells, the proteases in both the basal and suprabasal cells, and the lipid and steroid metabolism enzymes in suprabasal cells responsible for the permeability barrier. We identified the signaling pathways specific for the two populations and found two previously unknown paracrine and one juxtacrine signaling pathway operating between the basal and suprabasal cells. Furthermore, using specific expression signatures, we identified a new set of late differentiation markers and mapped their chromosomal loci, as well as a new set of melanocyte-specific markers. The data represent a quantum jump in understanding the mechanisms of epidermal differentiation.

  20. Nickel-responsive transcriptional regulators.

    Science.gov (United States)

    Musiani, Francesco; Zambelli, Barbara; Bazzani, Micaela; Mazzei, Luca; Ciurli, Stefano

    2015-09-01

    Nickel is an essential micronutrient for a large number of living organisms, but it is also a toxic metal ion when it accumulates beyond the sustainable level as it may result if and when its cellular trafficking is not properly governed. Therefore, the homeostasis and metabolism of nickel is tightly regulated through metal-specific protein networks that respond to the available Ni(II) concentration. These are directed by specific nickel sensors, able to couple Ni(II) binding to a change in their DNA binding affinity and/or specificity, thus translating the cellular level of Ni(II) into a modification of the expression of the proteins devoted to modulating nickel uptake, efflux and cellular utilization. This review describes the Ni(II)-dependent transcriptional regulators discovered so far, focusing on their structural features, metal coordination modes and metal binding thermodynamics. Understanding these properties is essential to comprehend how these sensors correlate nickel availability to metal coordination and functional responses. A broad and comparative study, described here, reveals some general traits that characterize the binding stoichiometry and Ni(II) affinity of these metallo-sensors.

  1. Academic Training: String Theory

    CERN Multimedia

    Françoise Benz

    2005-01-01

    2004-2005 ACADEMIC TRAINING PROGRAMME LECTURE SERIES 6, 7, 8, 9 and 10 June from 11.00 to 12.00 hrs - Auditorium, bldg. 500 on 6, 7, 8 & 10 June, TH Auditorium, bldg. 4, 3rd floor on 9 June String Theory by C. Johnson / Univ. of Southern California, USA ENSEIGNEMENT ACADEMIQUE ACADEMIC TRAINING Françoise Benz 73127 academic.training@cern.ch If you wish to participate in one of the following courses, please discuss with your supervisor and apply electronically directly from the course description pages that can be found on the Web at: http://www.cern.ch/Training/ or fill in an 'application for training' form available from your Divisional Secretariat or from your DTO (Divisional Training Officer). Applications will be accepted in the order of their receipt.

  2. Cross cultural training

    Institute of Scientific and Technical Information of China (English)

    王容

    2014-01-01

    Under the background of economic globalization, the globalization of human resources management determines the success of enterprise success or failure, in a sense, human resource is the first resource of the enterprise. Cross-cultural training is the core problems in global human resource management, it can let employees quickly familiar with the company's business and job content, understand the enterprise culture and core idea, to play a huge role in promoting enterprise development. Therefore, strengthen enterprise staff training, improve the comprehensive quality of enterprise personnel has become the urgent problems now in the process of enterprise development. Articles in samsung electronics of South Korea, for example, introduces the samsung especial y cross-cultural training content, training method and training effect. And analysis of cross-cultural training impact in the global business team and establish business relationship.

  3. Language Training: Anglais

    CERN Document Server

    Françoise Benz

    2004-01-01

    If you wish to participate in one of the following courses, please discuss with your supervisor and apply electronically directly from the course description pages that can be found on the Web at: http://www.cern.ch/Training/ or fill in an 'application for training' form available from your Divisional Secretariat or from your DTO (Divisional Training Officer). Applications will be accepted in the order of their receipt. Writing Professional Documents in English This course is designed for people with a good level of spoken English. Duration: 2 hours a week between 26 April and 1 July 2004. Timetable: Thursdays 12.00 to 14.00 Price: 440 CHF (for 8 students and 20 hours) For registration and further information, please consult our Web pages: http://cern.ch/Training or contact Mr. Liptow: tel. 72957 FORMATION EN LANGUES LANGUAGE TRAINING Françoise Benz 73127 language.training@cern.ch

  4. Safety training news

    CERN Multimedia

    Safety Training, HSE Unit

    2014-01-01

      SELF-RESCUE MASK The "Self-Rescue Mask" face-to-face training course has been replaced by a revised version. This measure concerns both the initial and the refresher course. For personnel who have successfully attended the initial or refresher Self-Rescue Mask training within the last three years, their Self-Rescue Mask training will still be valid.  The course description and registration form can be found in the training catalogue on the Safety Training Service’s website or catalogue. The Self-Rescue Mask training course is a mandatory prerequisite for following the new "Portable ODH Detector" e-learning course.   PORTABLE ODH DETECTOR A new e-learning awareness course, "Portable ODH Detector", is available via the SIR application on CERN’s intranet. Personnel requiring a portable ODH detector of the DRÄGER x-am 5000 type to allowed access th...

  5. Virtual Training Devices Laboratory

    Data.gov (United States)

    Federal Laboratory Consortium — The Virtual Training Devices (VTD) Laboratory at the Life Cycle Software Engineering Center, Picatinny Arsenal, provides a software testing and support environment...

  6. Universal Drive Train Facility

    Data.gov (United States)

    Federal Laboratory Consortium — This vehicle drive train research facility is capable of evaluating helicopter and ground vehicle power transmission technologies in a system level environment. The...

  7. Transcription-dependent degradation controls the stability of the SREBP family of transcription factors.

    Science.gov (United States)

    Sundqvist, Anders; Ericsson, Johan

    2003-11-25

    Cholesterol metabolism is tightly controlled by members of the sterol regulatory element-binding protein (SREBP) family of transcription factors. Here we demonstrate that the ubiquitination and degradation of SREBPs depend on their transcriptional activity. Mutations in the transactivation or DNA-binding domains of SREBPs inhibit their transcriptional activity and stabilize the proteins. The transcriptional activity and degradation of these mutants are restored when fused to heterologous transactivation or DNA-binding domains. When SREBP1a was fused to the DBD of Gal4, the ubiquitination and degradation of the fusion protein depended on coexpression of a promoter-reporter gene containing Gal4-binding sites. In addition, disruption of the interaction between WT SREBP and endogenous p300/CBP resulted in inhibition of SREBP-dependent transcription and stabilization of SREBP. Chemical inhibitors of transcription reduced the degradation of transcriptionally active SREBP1a, whereas they had no effect on the stability of transcriptionally inactive mutants, demonstrating that transcriptional activation plays an important role in the degradation of SREBPs. Thus, transcription-dependent degradation of SREBP constitutes a feedback mechanism to regulate the expression of genes involved in cholesterol metabolism and may represent a general mechanism to regulate the duration of transcriptional responses.

  8. Intermittent Transcription Dynamics for the Rapid Production of Long Transcripts of High Fidelity

    Directory of Open Access Journals (Sweden)

    Martin Depken

    2013-10-01

    Full Text Available Normal cellular function relies on the efficient and accurate readout of the genetic code. Single-molecule experiments show that transcription and replication are highly intermittent processes that are frequently interrupted by polymerases pausing and reversing directions. Although intermittent dynamics in replication are known to result from proofreading, their origin and significance during transcription remain controversial. Here, we theoretically investigate transcriptional fidelity and show that the kinetic scheme provided by the RNA-polymerase backtracking and transcript-cleavage pathway can account for measured error rates. Importantly, we find that intermittent dynamics provide an enormous increase in the rate of producing long transcripts of high fidelity. Our results imply that intermittent dynamics during transcription may have evolved as a way to mitigate the competing demands of speed and fidelity in the transcription of extended sequences.

  9. Evolution and diversification of the basal transcription machinery.

    Science.gov (United States)

    Duttke, Sascha H C

    2015-03-01

    Transcription initiation was once thought to be regulated primarily by sequence-specific transcription factors with the basal transcription machinery being largely invariant. Gradually it became apparent that the basal transcription machinery greatly diversified during evolution and new studies now demonstrate that diversification of the TATA-binding protein (TBP) family yielded specialized and largely independent transcription systems.

  10. Criterion-based laparoscopic training reduces total training time

    NARCIS (Netherlands)

    Brinkman, W.M.; Buzink, S.N.; Alevizos, L.; De Hingh, I.H.J.T.; Jakimowicz, J.J.

    2011-01-01

    The benefits of criterion-based laparoscopic training over time-oriented training are unclear. The purpose of this study is to compare these types of training based on training outcome and time efficiency. Methods During four training sessions within 1 week (one session per day) 34 medical interns (

  11. Criterion-based laparoscopic training reduces total training time

    NARCIS (Netherlands)

    Brinkman, W.M.; Buzink, S.N.; Alevizos, L.; De Hingh, I.H.J.T.; Jakimowicz, J.J.

    2012-01-01

    The benefits of criterion-based laparoscopic training over time-oriented training are unclear. The purpose of this study is to compare these types of training based on training outcome and time efficiency. Methods During four training sessions within 1 week (one session per day) 34 medical interns (

  12. Prunus transcription factors: Breeding perspectives

    Directory of Open Access Journals (Sweden)

    Valmor João Bianchi

    2015-06-01

    Full Text Available Many plant processes depend on differential gene expression, which is generally controlled by complex proteins called transcription factors (TFs. In peach, 1,533 TFs have been identified, accounting for about 5.5% of the 27,852 protein-coding genes. These TFs are the reference for the rest of the Prunus species. TF studies in Prunus have been performed on the gene expression analysis of different agronomic traits, including control of the flowering process, fruit quality, and biotic and abiotic stress resistance. These studies, using quantitative RT-PCR, have mainly been performed in peach, and to a lesser extent in other species, including almond, apricot, black cherry, Fuji cherry, Japanese apricot, plum, and sour and sweet cherry. Other tools have also been used in TF studies, including cDNA-AFLP, LC-ESI-MS, RNA and DNA blotting or mapping. More recently, new tools assayed include microarray and high-throughput DNA sequencing (DNA-Seq and RNA sequencing (RNA-Seq. New functional genomics opportunities include genome resequencing and the well-known synteny among Prunus genomes and transcriptomes. These new functional studies should be applied in breeding programs in the development of molecular markers. With the genome sequences available, some strategies that have been used in model systems (such as SNP genotyping assays and genotyping-by-sequencing may be applicable in the functional analysis of Prunus TFs as well. In addition, the knowledge of the gene functions and position in the peach reference genome of the TFs represents an additional advantage. These facts could greatly facilitate the isolation of genes via QTL (quantitative trait loci map-based cloning in the different Prunus species, following the association of these TFs with the identified QTLs using the peach reference genome.

  13. Nutrient Administration and Resistance Training

    Directory of Open Access Journals (Sweden)

    Leutholtz Brian

    2005-06-01

    Full Text Available Abstract Skeletal muscle tissue is tightly regulated throughout our bodies by balancing its synthesis and breakdown. Many factors are known to exist that cause profound changes on the overall status of skeletal muscle, some of which include exercise, nutrition, hormonal influences and disease. Muscle hypertrophy results when protein synthesis is greater than protein breakdown. Resistance training is a popular form of exercise that has been shown to increase muscular strength and muscular hypertrophy. In general, resistance training causes a stimulation of protein synthesis as well as an increase in protein breakdown, resulting in a negative balance of protein. Providing nutrients, specifically amino acids, helps to stimulate protein synthesis and improve the overall net balance of protein. Strategies to increase the concentration and availability of amino acids after resistance exercise are of great interest and have been shown to effectively increase overall protein synthesis. 123 After exercise, providing carbohydrate has been shown to mildly stimulate protein synthesis while addition of free amino acids prior to and after exercise, specifically essential amino acids, causes a rapid pronounced increase in protein synthesis as well as protein balance.13 Evidence exists for a dose-response relationship of infused amino acids while no specific regimen exists for optimal dosing upon ingestion. Ingestion of whole or intact protein sources (e.g., protein powders, meal-replacements has been shown to cause similar improvements in protein balance after resistance exercise when compared to free amino acid supplements. Future research should seek to determine optimal dosing of ingested intact amino acids in addition to identifying the cellular mechanistic machinery (e.g. transcriptional and translational mechanisms for causing the increase in protein synthesis.

  14. Positive reinforcement training in rhesus macaques-training progress as a result of training frequency.

    Science.gov (United States)

    Fernström, A-L; Fredlund, H; Spångberg, M; Westlund, K

    2009-05-01

    Positive reinforcement training (PRT) efficiency was examined as a function of training frequency in 33 pair- or triple-housed female rhesus macaques. The animals were trained three times a week, once a day or twice a day, using PRT and a clicker as a secondary reinforcer. All animals were trained on 30 sessions, with an average of 5 min per training session per animal. The behaviors, trained in succession, were Targeting (reliably touching and following a Target); Collaborating (dominant animals allowing subordinates to train while stationing); Box-training (accepting being enclosed in a small compartment while responding to Target training) and initial Injection training.Fulfilled criteria for Targeting were obtained in 32/33 animals in a median of nine training sessions. Collaboration was obtained in 27/33 animals in a median of 15 training sessions. However, only four animals completed Box-training during the 30 training sessions and started Injection training. When comparing training success in terms of number of training sessions, training twice a day was less efficient than the other two treatments. In terms of daily progress, our results suggest that from a management perspective, daily training is more conducive to quick training success than thrice weekly training. In addition, in this study no further advantages could be gained from training twice a day.

  15. Relational goods in training university groups: A research

    Directory of Open Access Journals (Sweden)

    Pietra Daniela Di Paola

    2014-09-01

    Full Text Available The aim of the study is to identify relational goods in the transcriptions produced by sound recording of a median group experience at University of Palermo. In particular, the present work proposes to analyze the most representative qualities of this phenomenon. The group becomes the elective setting where take place activities promoting professional training and encourages emerging of inter subjective space of relational learning. In this way, the story of relationship between individuals in a contest represents the central lump from which develop personal well-being and the capability to optimize human resources.Keywords: Relational good; Median training group; Well-being

  16. Nuclear Actin in Development and Transcriptional Reprogramming.

    Science.gov (United States)

    Misu, Shinji; Takebayashi, Marina; Miyamoto, Kei

    2017-01-01

    Actin is a highly abundant protein in eukaryotic cells and dynamically changes its polymerized states with the help of actin-binding proteins. Its critical function as a constituent of cytoskeleton has been well-documented. Growing evidence demonstrates that actin is also present in nuclei, referred to as nuclear actin, and is involved in a number of nuclear processes, including transcriptional regulation and chromatin remodeling. The contribution of nuclear actin to transcriptional regulation can be explained by its direct interaction with transcription machineries and chromatin remodeling factors and by controlling the activities of transcription factors. In both cases, polymerized states of nuclear actin affect the transcriptional outcome. Nuclear actin also plays an important role in activating strongly silenced genes in somatic cells for transcriptional reprogramming. When these nuclear functions of actin are considered, it is plausible to speculate that nuclear actin is also implicated in embryonic development, in which numerous genes need to be activated in a well-coordinated manner. In this review, we especially focus on nuclear actin's roles in transcriptional activation, reprogramming and development, including stem cell differentiation and we discuss how nuclear actin can be an important player in development and cell differentiation.

  17. Notation systems for transcription: an empirical investigation.

    Science.gov (United States)

    Romero, Catherine; O'Connell, Daniel C; Kowal, Sabine

    2002-11-01

    A 21-syllable question posed by Bernard Shaw in a CNN television interview with Margaret Thatcher was presented to 90 participants, either as an audio recording or as a typed transcript or as both. Participants were asked to speak it, as closely as possible, as Shaw had (or, in conditions without the audio recording, as he might have). The typed version was either an ordinary transcript or a transcript in one of three transcription systems used currently in research on spoken discourse, all of which incorporate notations for prosody. Hence, there were nine conditions in all, with five women and five men in each. Contrary to the experimental hypothesis, approximations to Shaw's original temporal measures of performance were not degraded but were instead improved significantly by the addition of a prosodically notated transcript to the audio recording and significantly more in the absence of the audio recording. Presentation of the ordinary transcript alone produced the worst approximation to Shaw's temporal measures. The usefulness, accuracy, and readability of transcripts prepared according to detailed notation systems are discussed.

  18. Outsourcing of Training

    Energy Technology Data Exchange (ETDEWEB)

    GAMIN, J.E.

    1999-06-17

    Several lessons are covered that has been learned at Fluor Daniel Hanford during their outsourcing effort. We have overcome problems because of our joint commitment to success. We continue to work our way through issues as they arise. On the positive side, several good things have happened as a result of outsourcing. Our training provider (EXITECH) has proven to be very responsive to our many different customers. We have been able to establish and support a 6-month training schedule for the first time. This has allowed the different facilities who send workers to training with more flexibility in planning their workers' time away from their jobs. In addition to the master training schedule, each facility on the Hanford Site has its own special or emergent training needs, and EXITECH has been very responsive in meeting these individual facilities' needs. Student and supervisor feedback on the quality and effectiveness of environmental, safety and health training provided by EXITECH has been very positive. Worker and supervisor feedback indicates the ''hands-on'' training serves their needs, and focuses on what they need to know to be able to perform work safely and efficiently. EXITECH strives very hard at presenting training so students can become successful in their training efforts. The workforce has noticed EXITECH's training philosophy is based on shared success with the trainee, not failure. Along the way, FDH has saved money as a result of this outsourcing. During our first year of the contract, we estimated a $400,000 savings. The decision as to outsource training is likely the most important you will make for your organization. In our situation, it fit the business goals we had established in our bid for the Hanford contract. However, if we were to begin again, we would factor some of the valuable lessons learned experienced during the first 16 months of our current outsourcing.

  19. Proactive driver training program

    Energy Technology Data Exchange (ETDEWEB)

    Vossler, W. [Kinetic Safety Consulting Inc., Grande Prairie, AB (Canada)

    2005-07-01

    Skid avoidance training is a recent approach to driver training and has been employed in various countries with a high degree of success. Among top ranked countries, motor vehicle incidents trends indicate higher incident rates among drivers are often due to lack of knowledge, experience and risk awareness. If lowered age limit experience is attained under direct supervision and in safe training conditions, it was suggested, incident frequency is reduced. A Norway study confirmed an increase in vehicle incident rates after drivers had received skid control training. The drivers were unable to maintain skill levels needed to react to critical driving tasks and had unrealistic expectations of skill after training. However, a skid avoidance training program launched in Sweden in 1999 has resulted in a 50 per cent reduction of vehicle incidents in the last 2 years. Details of the Skidcar System were presented, including details of the driving simulator, where simulation of actual driving situations is achieved by simply adjusting the amount of grip the vehicle has with the driving surface. Instructors modify driving behaviors based upon the driver's ability to maintain grip. There are over 200 units in North America. In addition, a Proactive Light Vehicle Driver Training/ Heavy Vehicle Assessment Program was initiated in 2003, with a motor vehicle incident rate reduction of 50 per cent at the end of 2004. Various examples of situations in which drivers have used their skid avoidance skills to avoid incidents were included. It was noted that the trend among driver training professionals has been towards decision-based rather than skills-based training, as skills-based training will diminish over time, and requires frequent re-training periods. Cognitive and perceptual skills were examined, as well as cognitive, associative and autonomous learning phases. It was concluded that skid avoidance is largely a decision-based skill. tabs, figs.

  20. Management of nuclear training center

    Energy Technology Data Exchange (ETDEWEB)

    Seo, In Suk; Lee, Han Young; Cho, Boung Jae; Lee, Seung Hee; Lee, Eoi Jin; You, Byung Hoon; Lee, Won Ku; Jeon, Hyung Ryeon; Seo, Kyung Won; Kim, Young Joong; Kim, Ik Hyun; Hyun, Ha Il; Choi, Il Ki; Hong, Choon Sun; Won, Jong Yeul; Joo, Yong Chang; Nam, Jae Yeul; Sin, Eun Jeong [Korea Atomic Energy Research Institute, Taejon (Korea, Republic of)

    1996-02-01

    This report describes the annual results of training courses. The scope and contents are as follows : 1. Regional and interregional training courses, 2. Training courses assisted by foreign experts, 3. Training courses for nuclear industry personnel, 4. Training courses for internal staff-members, 5. Training courses under the law. The nuclear training center executed the open-door training courses for 2,699 engineers/scientists from the regulatory body, nuclear industries, research institutes and other related organizations by means of offering 69 training courses during the fiscal year 1995. (Author) .new.

  1. Folding Kinetics of Riboswitch Transcriptional Terminators

    Science.gov (United States)

    Sauerwine, Benjamin; Widom, Michael

    2009-03-01

    Riboswitches control the expression of genes in bacteria by halting gene transcription or allowing it to proceed based on the presence of ligands in solution. A key feature of every riboswitch is a transcriptional terminator in which the messenger RNA folds into a secondary structure with the stem-loop structure of a hairpin. Through kinetic Monte Carlo simulation we show that terminators have been naturally selected to fold with high reliability on the time-scale of gene transcription. This efficient folding behavior is preserved among two classes of riboswitch and among two species of bacteria.

  2. Our evolving knowledge of the transcriptional landscape.

    Science.gov (United States)

    Hume, David A

    2008-01-01

    The development of a genome-scale approach to identification of the 5' ends of capped mRNAs (CAGE) has given new insights into many aspects of mammalian RNApolII transcription control. They include the identification of the minimal initiator motif, the different types of proximal promoter architecture, the promoters of noncoding RNAs, the transcription of retrotransposons, and the extensive impact of alternative promoters on the proteome. CAGE also offers applications as a form of expression profiling that measures promoter use, allowing more precise development of transcriptional network models.

  3. CHD chromatin remodelers and the transcription cycle.

    Science.gov (United States)

    Murawska, Magdalena; Brehm, Alexander

    2011-01-01

    It is well established that ATP-dependent chromatin remodelers modulate DNA access of transcription factors and RNA polymerases by "opening" or "closing" chromatin structure. However, this view is far too simplistic. Recent findings have demonstrated that these enzymes not only set the stage for the transcription machinery to act but are actively involved at every step of the transcription process. As a consequence, they affect initiation, elongation, termination and RNA processing. In this review we will use the CHD family as a paradigm to illustrate the progress that has been made in revealing these new concepts.

  4. Optogenetic control of transcription in zebrafish.

    Directory of Open Access Journals (Sweden)

    Hongtao Liu

    Full Text Available Light inducible protein-protein interactions are powerful tools to manipulate biological processes. Genetically encoded light-gated proteins for controlling precise cellular behavior are a new and promising technology, called optogenetics. Here we exploited the blue light-induced transcription system in yeast and zebrafish, based on the blue light dependent interaction between two plant proteins, blue light photoreceptor Cryptochrome 2 (CRY2 and the bHLH transcription factor CIB1 (CRY-interacting bHLH 1. We demonstrate the utility of this system by inducing rapid transcription suppression and activation in zebrafish.

  5. Can Trains Really Float?

    Science.gov (United States)

    McCartney, Robin Ward; Deroche, Sarah; Pontiff, Danielle

    2008-01-01

    Have you ever heard of a Maglev train? Who would be crazy enough to think that exploring how a high-tech train little known in the United States works with a group of fourth-grade students would yield understandings about the properties of magnetism, force and motion, and inquiry science? Fortunately, the authors--a college methods professor and…

  6. Constructing Training Demonstrations

    Science.gov (United States)

    2009-01-16

    training (BMT) is one of the most extensively used training methods available to modern organizations [25]. BMT is based on Bandura’s social learning theory [13...Utilizing the model provided by social learning theory , BMT includes processes such as modeling, a retention process, behavioral rehearsal

  7. Job Instruction Training.

    Science.gov (United States)

    Pfau, Richard H.

    Job Instruction Training (JIT) is a step-by-step, relatively simple technique used to train employees on the job. It is especially suitable for teaching manual skills or procedures; the trainer is usually an employee's supervisor but can be a co-worker. The JIT technique consists of a series of steps that a supervisor or other instructor follows…

  8. Training the Trainer.

    Science.gov (United States)

    Veri, Clive C.; Vonder Haar, T. A.

    Guidelines are furnished for application of psychological theory (including principles of learning, motivation, and reinforcement) in the training room, job or task analysis, formation of learning objectives, and selection of appropriate training techniques and audiovisual aids. The importance of clear communication and a supportive learning…

  9. Training Recurrent Networks

    DEFF Research Database (Denmark)

    Pedersen, Morten With

    1997-01-01

    Training recurrent networks is generally believed to be a difficult task. Excessive training times and lack of convergence to an acceptable solution are frequently reported. In this paper we seek to explain the reason for this from a numerical point of view and show how to avoid problems when...

  10. Training a cataract surgeon

    Directory of Open Access Journals (Sweden)

    M Babar Qureshi

    2014-07-01

    Full Text Available Training in cataract surgery is one of the key factors needed to ensure high quality cataract surgery with good visual outcomes and patient satisfaction. The training has to impart the right skills to the right person by the right trainer and in the right environment.

  11. Trains across the USA

    Science.gov (United States)

    McIntosh, Phyllis

    2013-01-01

    This feature article explores the topic of trains from their early history to recent trends in railroading. A glossary provides related vocabulary, a sidebar called "Romance of the Rails" adds colorful detail about train songs and railroad lore. An additional section, "Classroom Activities," by John Silver, presents three…

  12. Training Restricted Boltzmann Machines

    DEFF Research Database (Denmark)

    Fischer, Asja

    Restricted Boltzmann machines (RBMs) are probabilistic graphical models that can also be interpreted as stochastic neural networks. Training RBMs is known to be challenging. Computing the likelihood of the model parameters or its gradient is in general computationally intensive. Thus, training...

  13. Simulators in driver training.

    NARCIS (Netherlands)

    2009-01-01

    In 2010, about 150 driving simulators were being used for the basic driver training in the Netherlands. According to theories about how people learn, simulator training has both advantages and disadvantages. In order to be able to learn something from a simulator, its technical quality must be adequ

  14. Visual Impairment Training Reviewed.

    Science.gov (United States)

    Maychell, Karen; Smart, David

    1989-01-01

    A British study of training provided for social services workers with the visually impaired found that emphasis was on workers' learning the skills their clients would need, rather than on how to teach those skills; most training is geared toward the totally blind, a small proportion of the population; and workers felt the need to acquire…

  15. All aboard the Safety Train(ing)!

    CERN Multimedia

    Rosaria Marraffino

    2015-01-01

    Would you like to influence CERN’s safety courses? Do you want to help build better training courses? If your answer is yes to one or both of these questions: now is the time! The Safety Training section is looking for volunteers from the whole CERN community to test new courses before they go online for all members of the personnel.    The Safety Training section is redesigning the CERN e-learning package in order to adopt a more educational approach and to make the courses a more enjoyable experience. The section is now calling for volunteers. “We know we can do much more with testers’ help and feedback,” explains Christoph Balle, Safety Training section leader. “By having the end users actively involved in the process, we’ll achieve our goal of communicating safety in the best possible way. As the volunteers will play an active role in the development of the courses, they will be providing a service to the whole community.&am...

  16. Academic Training: 2004 - 2005 ACADEMIC TRAINING PROGRAMME

    CERN Multimedia

    Françoise Benz

    2004-01-01

    1st Term - 01 October to 17 December 2004 REGULAR LECTURE PROGRAMME New Trends in Fusion Research by A. Fasoli, EPFL, Lausanne, CH 11, 12, 13 October Physics at e+e- linear collider by K. Desch, DESY, Hamburg, D 15, 16, 17, 18, 19 November LECTURE SERIES FOR POSTGRADUATE STUDENTS Standard Model by R. Barbieri, CERN-PH-TH 6, 7, 8, 9 10 December The lectures are open to all those interested, without application. The abstract of the lectures, as well as any change to the above information (title, dates, time, place etc) will be published in the CERN Bulletin, the WWW, and by notices before each term and for each series of lectures. ENSEIGNEMENT ACADEMIQUE ACADEMIC TRAINING Françoise Benz 73127 academic.training@cern.ch If you wish to participate in one of the following courses, please discuss with your supervisor and apply electronically directly from the course description pages that can be found on the Web at: http://www.cern.ch/Training/ or fill in an 'application for training' form a...

  17. Intercultural Training: Six Measures Assessing Training Needs

    Science.gov (United States)

    Graf, Andrea; Mertesacker, Marion

    2009-01-01

    Purpose: The purpose of this paper is to develop recommendations for measures assessing intercultural training needs for international human resource management. Based on scientific as well as application-oriented criteria the aim is to select six measures assessing general intercultural competencies and with the help of behaviour ratings in…

  18. Do Sectoral Training Funds Stimulate Training?

    Science.gov (United States)

    Kamphuis, Pascal; Glebbeek, Arie C.; Van Lieshout, Harm

    2010-01-01

    Sectoral levelling funds are an arrangement aimed at alleviating a well-known theoretical problem of underinvestment in worker training because of free-rider behaviour of firms. In the Netherlands, collective agreements require firms to participate in such funds in a number of sectors. Using a comprehensive dataset of Dutch firms, we attempt to…

  19. Training Public Health Advisors.

    Science.gov (United States)

    Meyer, Pamela A; Brusuelas, Kristin M; Baden, Daniel J; Duncan, Heather L

    2015-01-01

    Federal public health advisors provide guidance and assistance to health departments to improve public health program work. The Centers for Disease Control and Prevention (CDC) prepares them with specialized training in administering public health programs. This article describes the evolving training and is based on internal CDC documents and interviews. The first federal public health advisors worked in health departments to assist with controlling syphilis after World War II. Over time, more CDC prevention programs hired them. To meet emerging needs, 3 major changes occurred: the Public Health Prevention Service, a fellowship program, in 1999; the Public Health Associate Program in 2007; and integration of those programs. Key components of the updated training are competency-based training, field experience, supervision, recruitment and retention, and stakeholder support. The enduring strength of the training has been the experience in a public health agency developing practical skills for program implementation and management.

  20. LEAH interdisciplinary training program.

    Science.gov (United States)

    Robbins, Cynthia L; Rickert, Vaughn D

    2016-08-01

    This article describes the Leadership Education in Adolescent Health (LEAH) interdisciplinary training program in the United States. The Maternal and Child Health Bureau authorized by legislation provides monies to train leaders in adolescent health through a competitive grant process. Currently, seven academic medical centers have funding to provide leadership in adolescent health (LEAH) training in five core disciplines: medicine, nursing, psychology, social work and nutrition. LEAH training programs both ensure high clinical competence in core disciplines serving adolescents and prepare trainees for leadership positions in adolescent health and public health care realms. Together, these programs trained almost 1000 long-term trainees across these five disciplines, and graduates from these programs are working in 45 of the 50 states within the United States. About 90% of these graduates are working with maternal and child/adolescent health populations, and almost all have held leadership positions in the areas of public health, advocacy, public policy, academic medical centers and/or clinical care settings.

  1. Monitoring spike train synchrony

    CERN Document Server

    Kreuz, Thomas; Houghton, Conor; Andrzejak, Ralph G; Mormann, Florian

    2012-01-01

    Recently, the SPIKE-distance has been proposed as a parameter-free and time-scale independent measure of spike train synchrony. This measure is time-resolved since it relies on instantaneous estimates of spike train dissimilarity. However, its original definition led to spuriously high instantaneous values for event-like firing patterns. Here we present a substantial improvement of this measure which eliminates this shortcoming. The reliability gained allows us to track changes in instantaneous clustering, i.e., time-localized patterns of (dis)similarity among multiple spike trains. Additional new features include selective and triggered temporal averaging as well as the instantaneous comparison of spike train groups. In a second step, a causal SPIKE-distance is defined such that the instantaneous values of dissimilarity rely on past information only so that time-resolved spike train synchrony can be estimated in real-time. We demonstrate that these methods are capable of extracting valuable information from ...

  2. Time Use on Trains

    DEFF Research Database (Denmark)

    Bjørner, Thomas

    2016-01-01

    This study explores how travel time is used and how passengers conceptualise travel time in Danish intercity trains and intercity fast trains. The new contribution to the literature this study can offer is in the inclusion of all kinds of passengers in the different compartments to understand train...... travel as a dynamic act of moving with shifts in activities. A mixed-method approach is used with self-completed questionnaires, frequency observations, shadowing observations and interviews. The findings reveal that the train passengers’ acts on the move are framed by both macro- and microstructures....... The passengers create a travel space in which they make dynamic shifts in different kinds of activities: media use, media non-use, social interactions and non-social interactions. Passengers expect the train operator to provide the travel space for different activities (including the possibility of mobile...

  3. Technical Training: Places available

    CERN Multimedia

    Monique Duval

    2004-01-01

    If you wish to participate in one of the following courses, please discuss with your supervisor and apply electronically directly from the course description pages that can be found on the Web at: http://www.cern.ch/Training/ or fill in an 'application for training' form available from your Divisional Secretariat or from your DTO (Divisional Training Officer). Applications will be accepted in the order of their receipt. The number of places available may vary. Please check our Web site to find out the current availability. Places are available in the following courses: The Joint PVSS JCOP Framework: 14 - 18.6.2004 (5 days) EXCEL 2003 - niveau 2 : 17 & 18.6.2004 (2 jours) MAGNE-04 : Magnétisme pour l'électrotechnique : 6 au 8.7.2004 (3 jours) Technical Training Monique Duval - Tel.74924 technical.training@cern.ch

  4. lncScore: alignment-free identification of long noncoding RNA from assembled novel transcripts

    Science.gov (United States)

    Zhao, Jian; Song, Xiaofeng; Wang, Kai

    2016-01-01

    RNA-Seq based transcriptome assembly has been widely used to identify novel lncRNAs. However, the best-performing transcript reconstruction methods merely identified 21% of full-length protein-coding transcripts from H. sapiens. Those partial-length protein-coding transcripts are more likely to be classified as lncRNAs due to their incomplete CDS, leading to higher false positive rate for lncRNA identification. Furthermore, potential sequencing or assembly error that gain or abolish stop codons also complicates ORF-based prediction of lncRNAs. Therefore, it remains a challenge to identify lncRNAs from the assembled transcripts, particularly the partial-length ones. Here, we present a novel alignment-free tool, lncScore, which uses a logistic regression model with 11 carefully selected features. Compared to other state-of-the-art alignment-free tools (e.g. CPAT, CNCI, and PLEK), lncScore outperforms them on accurately distinguishing lncRNAs from mRNAs, especially partial-length mRNAs in the human and mouse datasets. In addition, lncScore also performed well on transcripts from five other species (Zebrafish, Fly, C. elegans, Rat, and Sheep). To speed up the prediction, multithreading is implemented within lncScore, and it only took 2 minute to classify 64,756 transcripts and 54 seconds to train a new model with 21,000 transcripts with 12 threads, which is much faster than other tools. lncScore is available at https://github.com/WGLab/lncScore. PMID:27708423

  5. Interactions of transcription factors with chromatin.

    Science.gov (United States)

    van Bakel, Harm

    2011-01-01

    Sequence-specific transcription factors (TFs) play a central role in regulating transcription initiation by directing the recruitment and activity of the general transcription machinery and accessory factors. It is now well established that many of the effects exerted by TFs in eukaryotes are mediated through interactions with a host of coregulators that modify the chromatin state, resulting in a more open (in case of activation) or closed conformation (in case of repression). The relationship between TFs and chromatin is a two-way street, however, as chromatin can in turn influence the recognition and binding of target sequences by TFs. The aim of this chapter is to highlight how this dynamic interplay between TF-directed remodelling of chromatin and chromatin-adjusted targeting of TF binding determines where and how transcription is initiated, and to what degree it is productive.

  6. Comparison of Transcription Factor Binding Site Models

    KAUST Repository

    Bhuyan, Sharifulislam

    2012-05-01

    Modeling of transcription factor binding sites (TFBSs) and TFBS prediction on genomic sequences are important steps to elucidate transcription regulatory mechanism. Dependency of transcription regulation on a great number of factors such as chemical specificity, molecular structure, genomic and epigenetic characteristics, long distance interaction, makes this a challenging problem. Different experimental procedures generate evidence that DNA-binding domains of transcription factors show considerable DNA sequence specificity. Probabilistic modeling of TFBSs has been moderately successful in identifying patterns from a family of sequences. In this study, we compare performances of different probabilistic models and try to estimate their efficacy over experimental TFBSs data. We build a pipeline to calculate sensitivity and specificity from aligned TFBS sequences for several probabilistic models, such as Markov chains, hidden Markov models, Bayesian networks. Our work, containing relevant statistics and evaluation for the models, can help researchers to choose the most appropriate model for the problem at hand.

  7. Contributions of nuclear architecture to transcriptional control.

    Science.gov (United States)

    Stein, G S; van Wijnen, A J; Stein, J; Lian, J B; Montecino, M

    1995-01-01

    Three parameters of nuclear structure contribute to transcriptional control. The linear representation of promoter elements provides competency for physiological responsiveness within the contexts of development as well as cycle- and phenotype-dependent regulation. Chromatin structure and nucleosome organization reduce distances between independent regulatory elements providing a basis for integrating components of transcriptional control. The nuclear matrix supports gene expression by imposing physical constraints on chromatin related to three-dimensional genomic organization. In addition, the nuclear matrix facilitates gene localization as well as the concentration and targeting of transcription factors. Several lines of evidence are presented that are consistent with involvement of multiple levels of nuclear architecture in cell growth and tissue-specific gene expression during differentiation. Growth factor and steroid hormone responsive modifications in chromatin structure, nucleosome organization, and the nuclear matrix that influence transcription of the cell cycle-regulated histone gene and the bone tissue-specific osteocalcin gene during progressive expression of the osteoblast phenotype are considered.

  8. Topologies for perfect adaptation in gene transcription

    Science.gov (United States)

    Shi, Wenjia; Tang, Chao

    2014-03-01

    Adaptation is commonly used in sensory systems and signaling networks to allow the detection of further stimuli. Despite enzymatic network topologies for adaptation have been investigated systematically, the topology of transcriptional network that could perform adaptation still remains unclear, due to the complexity of transcriptional regulation. Here, we systematically investigated all three-node transcriptional networks, and found the topologies of transcriptional networks for adaptation are different from that of enzymatic ones. While both negative feedback loop (NFBL) and incoherent feed forward loop (IFFL) are capable of performing adaptation analytically, a positive self-regulation on buffer node is necessary for NFBL topology and more flexible structures emerge for IFFL than that of enzymatic networks. Most of the simulation results agree with analytical predictions. This study may explain the mechanism of adapted gene regulation behavior and supply a design table for gene regulatory adaptation.

  9. Transcriptional networks and chromatin remodeling controlling adipogenesis

    DEFF Research Database (Denmark)

    Siersbæk, Rasmus; Nielsen, Ronni; Mandrup, Susanne

    2012-01-01

    remodeling have revealed 'snapshots' of this cascade and the chromatin landscape at specific time-points of differentiation. These studies demonstrate that multiple adipogenic transcription factors co-occupy hotspots characterized by an open chromatin structure and specific epigenetic modifications...

  10. Dynamics of transcription-translation networks

    Science.gov (United States)

    Hudson, D.; Edwards, R.

    2016-09-01

    A theory for qualitative models of gene regulatory networks has been developed over several decades, generally considering transcription factors to regulate directly the expression of other transcription factors, without any intermediate variables. Here we explore a class of models that explicitly includes both transcription and translation, keeping track of both mRNA and protein concentrations. We mainly deal with transcription regulation functions that are steep sigmoids or step functions, as is often done in protein-only models, though translation is governed by a linear term. We extend many aspects of the protein-only theory to this new context, including properties of fixed points, description of trajectories by mappings between switching points, qualitative analysis via a state-transition diagram, and a result on periodic orbits for negative feedback loops. We find that while singular behaviour in switching domains is largely avoided, non-uniqueness of solutions can still occur in the step-function limit.

  11. Cognitive training for dementia

    Directory of Open Access Journals (Sweden)

    Konta, Brigitte

    2005-12-01

    Full Text Available The aim of the HTA report is to evaluate the effectiveness of cognitive training methods to treat cognitive disorders of dementia and other diseases with cognitive deficits. For this purpose, a systematic literature search was carried out first based on the DIMDI superbase retrieval. The identified publications were judged and selected by two independent, methodically competent experts. 33 publications were included in the report. Based on the studies for a normal cognitive development in old age a theory that healthy older people have a considerable capacity reserve for an improved performance in abstract abilities of thinking can be assumed. The first symptoms for older people at risk for dementia are a reduced cognitive capacity reserve. Cognitive training methods therefore focus abilities of abstract memory. Apart from types of dementia another two groups of diseases with cognitive deficits were included in the HTA report: cerebral lesions and schizophrenic psychoses. Studies with mild as well as forms of dementia heavy forms including the Alzheimer disease were included. The described training methods were very heterogeneous with regard to their contents, the temporal sequence and the outcome parameter. The studies were methodically partly contestable. Approximately a third of the studies of all publications could show improvements in the cognitive achievements by the training. Three studies concerning cognitive training methods in case of cerebral lesions were included. All three studies demonstrated a significant improvement in the training group in some outcome parameters. Special cognitive training methods were used for the treatment of cognitive deficits at schizophrenic psychoses. The neurocognitive training (NET, the "Cognitive Remediation Therapy" as well as the strategic training with coaching proved to be effective. The studies, however, were hardly comparable and very heterogeneous in detail. Summarising the cognitive training

  12. Transcription Factors in Xylem Development. Final report

    Energy Technology Data Exchange (ETDEWEB)

    Sederoff, Ronald; Whetten, Ross; O' Malley, David; Campbell, Malcolm

    1999-07-01

    Answers to the following questions are answered in this report. do the two pine Byb proteins previously identified as candidate transcription factors bind to DNA and activate transcription? In what cell types are tehse Myb proteins expressed? Are these proteins localized to the nucleus? Do other proteins in pine xylem interact with these Myb proteins? Does altered expression of these genes have an impact on xylogenesis, specifically the expression of monolignol biosynthetic genes?

  13. Biophysical models of transcription in cells

    Science.gov (United States)

    Choubey, Sandeep

    Cells constantly face environmental challenges and deal with them by changing their gene expression patterns. They make decisions regarding which genes to express and which genes not to express based on intra-cellular and environmental cues. These decisions are often made by regulating the process of transcription. While the identities of the different molecules that take part in regulating transcription have been determined for a number of different genes, their dynamics inside the cell are still poorly understood. One key feature of these regulatory dynamics is that the numbers of the bio-molecules involved is typically small, resulting in large temporal fluctuations in transcriptional outputs (mRNA and protein). In this thesis I show that measurements of the cell-to-cell variability of the distribution of transcribing RNA polymerases along a gene provide a previously unexplored method for deciphering the mechanism of its transcription in vivo. First, I propose a simple kinetic model of transcription initiation and elongation from which I calculate transcribing RNA polymerase copy-number fluctuations. I test my theory against published data obtained for yeast genes and propose a novel mechanism of transcription. Rather than transcription being initiated through a single rate-limiting step, as was previously proposed, my single-cell analysis reveals the presence of at least two rate limiting steps. Second, I compute the distribution of inter-polymerase distance distribution along a gene and propose a method for analyzing inter-polymerase distance distributions acquired in experiments. By applying this method to images of polymerases transcribing ribosomal genes in E.coli I show that one model of regulation of these genes is consistent with inter-polymerase distance data while a number of other models are not. The analytical framework described in this thesis can be used to extract quantitative information about the dynamics of transcription from single

  14. Co-transcriptional splicing in two yeasts

    OpenAIRE

    Herzel, Lydia

    2015-01-01

    Cellular function and physiology are largely established through regulated gene expression. The first step in gene expression, transcription of the genomic DNA into RNA, is a process that is highly aligned at the levels of initiation, elongation and termination. In eukaryotes, protein-coding genes are exclusively transcribed by RNA polymerase II (Pol II). Upon transcription of the first 15-20 nucleotides (nt), the emerging nascent RNA 5’ end is modified with a 7-methylguanosyl cap. This is on...

  15. Strength Training and Your Child

    Science.gov (United States)

    ... Old Feeding Your 1- to 2-Year-Old Strength Training and Your Child KidsHealth > For Parents > Strength ... help prevent injuries and speed up recovery. About Strength Training Strength training is the practice of using ...

  16. Small Business's Big Training Needs.

    Science.gov (United States)

    Kelly, Leslie

    1984-01-01

    Discusses the training needs of small businesses in light of their high failure rate (50 percent). Includes characteristics of successful small businesses, potential training needs, training methods, and topics for seminars. (JOW)

  17. Structure and regulatory function of plant transcription factors

    Institute of Scientific and Technical Information of China (English)

    2001-01-01

    The expression of inducible genes in plants is regulated byspecific transcription factors at the transcriptional level. A typical transcription factor usually contains a DNA-binding domain, a transcription regulation domain, a dimerization site and a nuclear localization domain. These functional domains define the characteristic, localization and regulatory role of a transcription factor. Transcription factors recognize and bind to specific cis-acting elements or interact with other proteins, and then activate or repress the transcription of target genes by their functional domains. In recent years, elucidation on the structure and function of transcription factors has become an important subject in plant molecular biology.

  18. MADS-box gene evolution - structure and transcription patterns

    DEFF Research Database (Denmark)

    Johansen, Bo; Pedersen, Louise Buchholt; Skipper, Martin;

    2002-01-01

    Mads-box genes, ABC model, Evolution, Phylogeny, Transcription patterns, Gene structure, Conserved motifs......Mads-box genes, ABC model, Evolution, Phylogeny, Transcription patterns, Gene structure, Conserved motifs...

  19. The regulation of transcription in memory consolidation.

    Science.gov (United States)

    Alberini, Cristina M; Kandel, Eric R

    2014-12-04

    De novo transcription of DNA is a fundamental requirement for the formation of long-term memory. It is required during both consolidation and reconsolidation, the posttraining and postreactivation phases that change the state of the memory from a fragile into a stable and long-lasting form. Transcription generates both mRNAs that are translated into proteins, which are necessary for the growth of new synaptic connections, as well as noncoding RNA transcripts that have regulatory or effector roles in gene expression. The result is a cascade of events that ultimately leads to structural changes in the neurons that mediate long-term memory storage. The de novo transcription, critical for synaptic plasticity and memory formation, is orchestrated by chromatin and epigenetic modifications. The complexity of transcription regulation, its temporal progression, and the effectors produced all contribute to the flexibility and persistence of long-term memory formation. In this article, we provide an overview of the mechanisms contributing to this transcriptional regulation underlying long-term memory formation.

  20. DMD transcript imbalance determines dystrophin levels.

    Science.gov (United States)

    Spitali, Pietro; van den Bergen, Janneke C; Verhaart, Ingrid E C; Wokke, Beatrijs; Janson, Anneke A M; van den Eijnde, Rani; den Dunnen, Johan T; Laros, Jeroen F J; Verschuuren, Jan J G M; 't Hoen, Peter A C; Aartsma-Rus, Annemieke

    2013-12-01

    Duchenne and Becker muscular dystrophies are caused by out-of-frame and in-frame mutations, respectively, in the dystrophin encoding DMD gene. Molecular therapies targeting the precursor-mRNA are in clinical trials and show promising results. These approaches will depend on the stability and expression levels of dystrophin mRNA in skeletal muscles and heart. We report that the DMD gene is more highly expressed in heart than in skeletal muscles, in mice and humans. The transcript mutated in the mdx mouse model shows a 5' to 3' imbalance compared with that of its wild-type counterpart and reading frame restoration via antisense-mediated exon skipping does not correct this event. We also report significant transcript instability in 22 patients with Becker dystrophy, clarifying the fact that transcript imbalance is not caused by premature nonsense mutations. Finally, we demonstrate that transcript stability, rather than transcriptional rate, is an important determinant of dystrophin protein levels in patients with Becker dystrophy. We suggest that the availability of the complete transcript is a key factor to determine protein abundance and thus will influence the outcome of mRNA-targeting therapies.

  1. Control of transcription by cell size.

    Directory of Open Access Journals (Sweden)

    Chia-Yung Wu

    Full Text Available Cell size increases significantly with increasing ploidy. Differences in cell size and ploidy are associated with alterations in gene expression, although no direct connection has been made between cell size and transcription. Here we show that ploidy-associated changes in gene expression reflect transcriptional adjustment to a larger cell size, implicating cellular geometry as a key parameter in gene regulation. Using RNA-seq, we identified genes whose expression was altered in a tetraploid as compared with the isogenic haploid. A significant fraction of these genes encode cell surface proteins, suggesting an effect of the enlarged cell size on the differential regulation of these genes. To test this hypothesis, we examined expression of these genes in haploid mutants that also produce enlarged size. Surprisingly, many genes differentially regulated in the tetraploid are identically regulated in the enlarged haploids, and the magnitude of change in gene expression correlates with the degree of size enlargement. These results indicate a causal relationship between cell size and transcription, with a size-sensing mechanism that alters transcription in response to size. The genes responding to cell size are enriched for those regulated by two mitogen-activated protein kinase pathways, and components in those pathways were found to mediate size-dependent gene regulation. Transcriptional adjustment to enlarged cell size could underlie other cellular changes associated with polyploidy. The causal relationship between cell size and transcription suggests that cell size homeostasis serves a regulatory role in transcriptome maintenance.

  2. Mitochondrial transcription: is a pattern emerging?

    Science.gov (United States)

    Jaehning, J A

    1993-04-01

    Despite the striking similarities of RNA polymerases and transcription signals shared by eubacteria, archaebacteria and eukaryotes, there has been little indication that transcription in mitochondria is related to any previously characterized model. Only in yeast has the subunit structure of the mitochondrial RNA polymerase been determined. The yeast enzyme is composed of a core related to polymerases from bacteriophage T7 and T3, and a promoter recognition factor similar to bacterial sigma factors. Soluble systems for studying mitochondrial transcript initiation in vitro have been described from several organisms, and used to determine consensus sequences at or near transcription start sites. Comparison of these sequences from fungi, plants, and amphibians with the T7/T3 promoter suggests some intriguing similarities. Mammalian mitochondrial promoters do not fit this pattern but instead appear to utilize upstream sites, the target of a transcriptional stimulatory factor, to position the RNA polymerase. The recent identification of a possible homologue of the mammalian upstream factor in yeast mitochondria may indicate that a pattern will eventually be revealed relating the transcriptional machineries of all eukaryotic mitochondria.

  3. Transcriptional elongation factor ENL phosphorylated by ATM recruits polycomb and switches off transcription for DSB repair.

    Science.gov (United States)

    Ui, Ayako; Nagaura, Yuko; Yasui, Akira

    2015-05-07

    Transcription is repressed if a DNA double-strand break (DSB) is introduced in close proximity to a transcriptional activation site at least in part by H2A-ubiquitination. While ATM signaling is involved, how it controls H2A-ubiquitination remains unclear. Here, we identify that, in response to DSBs, a transcriptional elongation factor, ENL (MLLT1), is phosphorylated by ATM at conserved SQ sites. This phosphorylation increases the interaction between ENL and the E3-ubiquitin-ligase complex of Polycomb Repressive Complex 1 (PRC1) via BMI1. This interaction promotes enrichment of PRC1 at transcription elongation sites near DSBs to ubiquitinate H2A leading to transcriptional repression. ENL SQ sites and BMI1 are necessary for KU70 accumulation at DSBs near active transcription sites and cellular resistance to DSBs. Our data suggest that ATM-dependent phosphorylation of ENL functions as switch from elongation to Polycomb-mediated repression to preserve genome integrity.

  4. Employee Training: An International Perspective

    OpenAIRE

    Kapsalis, Constantine

    1997-01-01

    Canada’s training effort relative to the rest of the IALS countries, measured in terms of hours of training per employee, was found to be average. The average employee in Canada received 44 hours of training in 1994, similar to the hours of training per employee in Switzerland, the United States and Germany. However, Canada’s training effort was considerably less than the Netherlands (74 hours per employee). One finding of particular interest to Canada is the virtual equality of training ...

  5. Nuclear Manpower Training

    Energy Technology Data Exchange (ETDEWEB)

    Min, B. J.; Han, K. W.; Lee, H. Y. and others

    2006-01-15

    Through the project on nuclear human resources development in 2005, the Nuclear Training Center of KAERI has provided 67 nuclear education and training courses for 3,658 persons from the domestic nuclear related organizations such as Government Agencies, nuclear industries, R and D institutes, universities, and public as well as from IAEA Member States. In addition, 6 students (MS and Ph D.) have taken nuclear technology related courses offered by UST-KAERI. The project has developed 8 programs and 12 courses. They includes programs for IAEA training, bilateral education and training, and in-house training as well as courses dealing with maintenance of nuclear power plants and management of electricity generation, thermal-hydraulics nuclear hydrogen, nuclear safeguards, radiation emergency preparedness and etc. National and international cooperation has been promoted. For ANENT, test operation, data loading and revision of the web-portal have been undertaken. Also the web-portal operation system has been established. For FNCA, NTC has cooperated for the establishment of a model of human resource development and the exchange of information/materials. With WNU, the NTC has made an effort for hosting 2007 WNU Summer Institute. The infrastructure for nuclear education and training has been strengthened. Basic directions for providing the customers with better service, This includes showing kindness to the customer, renovation of the interior of training facilities, and upgrading of web-based management system for learning and using facilities of NTC. Other efforts have resulted in the publication of 25 course materials (10 for international courses and 15 for national courses), and the improvement of education and training equipment. The International Nuclear Training and Education Center (INTEC), which was opened in 2002, has hosted 296 international and domestic events in 2005.

  6. Physical Training and Cardioprotection

    Institute of Scientific and Technical Information of China (English)

    Ping Zhang; Keith L. March; Dongming Hou

    2003-01-01

    Experimental, clinical and epidemiologic studies have provided strong evidence that physical training has beneficial effects on cardiovascular health. Numerous investigations have demonstrated that exercise increases coronary blood flow and myocardial perfusion. Importantly, training also can stimulate angiogenesis and accelerate collateral vessel growth in animal models with coronary artery occlusion. Cardiac adaptation such as increased vascularity or capillary density has been evidenced after regular endurance exercises. More recently, several studies indicate that physical training induces high levels of myocardial heat shock protein and antioxidant protein expression, which may play an important role in myocardial protection against ischemia-reperfusion injury.

  7. Space Age Training

    Science.gov (United States)

    1996-01-01

    Teledyne Brown developed a computer-based interactive multimedia training system for use with the Crystal Growth Furnace in the U.S. Microgravity Laboratory-2 mission on the Space Shuttle. Teledyne Brown commercialized the system and customized it for PPG Industries Aircraft Products. The system challenges learners with role-playing scenarios and software-driven simulations engaging all the senses using text, video, animation, voice, sounds and music. The transfer of this technology to commercial industrial process training has resulted in significant improvements in effectiveness, standardization, and quality control, as well as cost reductions over the usual classroom and on-the- job training approaches.

  8. CERN Technical Training : Vista !

    CERN Multimedia

    HR Department

    2008-01-01

    You are running Vista on your new PC – or are planning to install it? The Technical Training service is organizing a half-day training course on the new features of the VISTA operating system. This course introduces the new interfaces and presents the new functionalities for people who are experienced in the use of Windows XP. The next bilingual sessions of this course will take place on 12 December 2008 and 30 January 2009. Register using our catalogue: http://cta.cern.ch/cta2/f?p=110:9 or contact us with your questions/comments at mailto:Technical.Training@cern.ch

  9. CERN Technical Training : Vista !

    CERN Multimedia

    HR Department

    2008-01-01

    Are you running Vista on your new PC – or are you planning to install it? The Technical Training service is organizing a half-day training course on the new features of the VISTA operating system. This course introduces the new interfaces and presents the new functionalities for people who are experienced in the use of Windows XP. The next bilingual sessions of this course will take place on 12 December 2008 and 30 January 2009. Register using our catalogue: http://cta.cern.ch/cta2/f?p=110:9 or contact us with your questions/comments at mailto:Technical.Training@cern.ch

  10. CERN Technical Training : Vista !

    CERN Multimedia

    HR Department

    2008-01-01

    Are you running Vista on your new PC – or are planning to install it? The Technical Training service is organizing a half-day training course on the new features of the VISTA operating system. This course introduces the new interfaces and presents the new functionalities for people who are experienced in the use of Windows XP. The next bilingual sessions of this course will take place on 12 December 2008 and 30 January 2009. Register using our catalogue: http://cta.cern.ch/cta2/f?p=110:9 or contact us with your questions/comments at Technical.Training@cern.ch

  11. CERN Technical Training : Vista !

    CERN Multimedia

    HR Department

    2009-01-01

    Are you running Vista on your new PC – or are you planning to install it? The Technical Training service is organizing a half-day training course on the new features of the VISTA operating system. This course introduces the new interfaces and presents the new functionalities for people who are experienced in the use of Windows XP. The next bilingual session of this course will take place on 30 January 2009. Register using our catalogue: http://cta.cern.ch/cta2/f?p=110:9 or contact us with your questions/comments at mailto:Technical.Training@cern.ch

  12. CERN Technical Training : Vista !

    CERN Multimedia

    HR Department

    2008-01-01

    You are running Vista on your new PC – or are planning to install it? The Technical Training service is organizing a half-day training on the new features of the VISTA operating system. This course introduces the new interfaces and presents the new functionalities for people who are experienced using Windows XP. The next bilingual sessions of this course will take place on December 12, 2008 and January 30, 2009. Register using our catalogue : http://cta.cern.ch/cta2/f?p=110:9 or contact us with your questions/comments at Technical.Training@cern.ch

  13. Team training/simulation.

    Science.gov (United States)

    Clark, Erin A S; Fisher, Janet; Arafeh, Julia; Druzin, Maurice

    2010-03-01

    Obstetrical emergencies require the rapid formation of a team with clear communication, strong leadership, and appropriate decision-making to ensure a positive patient outcome. Obstetric teams can improve their emergency response capability and efficiency through team and simulation training. Postpartum hemorrhage is an ideal model for team and simulation training, as postpartum hemorrhage requires a multidisciplinary team with the capability to produce a protocol-driven, rapid response. This article provides an overview of team and simulation training and focuses on applications within obstetrics, particularly preparation for postpartum hemorrhage.

  14. Auditory Integration Training

    Directory of Open Access Journals (Sweden)

    Zahra Jafari

    2002-07-01

    Full Text Available Auditory integration training (AIT is a hearing enhancement training process for sensory input anomalies found in individuals with autism, attention deficit hyperactive disorder, dyslexia, hyperactivity, learning disability, language impairments, pervasive developmental disorder, central auditory processing disorder, attention deficit disorder, depressin, and hyperacute hearing. AIT, recently introduced in the United States, and has received much notice of late following the release of The Sound of a Moracle, by Annabel Stehli. In her book, Mrs. Stehli describes before and after auditory integration training experiences with her daughter, who was diagnosed at age four as having autism.

  15. Microprocessor mediates transcriptional termination of long noncoding RNA transcripts hosting microRNAs.

    Science.gov (United States)

    Dhir, Ashish; Dhir, Somdutta; Proudfoot, Nick J; Jopling, Catherine L

    2015-04-01

    MicroRNAs (miRNAs) play a major part in the post-transcriptional regulation of gene expression. Mammalian miRNA biogenesis begins with cotranscriptional cleavage of RNA polymerase II (Pol II) transcripts by the Microprocessor complex. Although most miRNAs are located within introns of protein-coding transcripts, a substantial minority of miRNAs originate from long noncoding (lnc) RNAs, for which transcript processing is largely uncharacterized. We show, by detailed characterization of liver-specific lnc-pri-miR-122 and genome-wide analysis in human cell lines, that most lncRNA transcripts containing miRNAs (lnc-pri-miRNAs) do not use the canonical cleavage-and-polyadenylation pathway but instead use Microprocessor cleavage to terminate transcription. Microprocessor inactivation leads to extensive transcriptional readthrough of lnc-pri-miRNA and transcriptional interference with downstream genes. Consequently we define a new RNase III-mediated, polyadenylation-independent mechanism of Pol II transcription termination in mammalian cells.

  16. Dynamic analysis of stochastic transcription cycles.

    Directory of Open Access Journals (Sweden)

    Claire V Harper

    2011-04-01

    Full Text Available In individual mammalian cells the expression of some genes such as prolactin is highly variable over time and has been suggested to occur in stochastic pulses. To investigate the origins of this behavior and to understand its functional relevance, we quantitatively analyzed this variability using new mathematical tools that allowed us to reconstruct dynamic transcription rates of different reporter genes controlled by identical promoters in the same living cell. Quantitative microscopic analysis of two reporter genes, firefly luciferase and destabilized EGFP, was used to analyze the dynamics of prolactin promoter-directed gene expression in living individual clonal and primary pituitary cells over periods of up to 25 h. We quantified the time-dependence and cyclicity of the transcription pulses and estimated the length and variation of active and inactive transcription phases. We showed an average cycle period of approximately 11 h and demonstrated that while the measured time distribution of active phases agreed with commonly accepted models of transcription, the inactive phases were differently distributed and showed strong memory, with a refractory period of transcriptional inactivation close to 3 h. Cycles in transcription occurred at two distinct prolactin-promoter controlled reporter genes in the same individual clonal or primary cells. However, the timing of the cycles was independent and out-of-phase. For the first time, we have analyzed transcription dynamics from two equivalent loci in real-time in single cells. In unstimulated conditions, cells showed independent transcription dynamics at each locus. A key result from these analyses was the evidence for a minimum refractory period in the inactive-phase of transcription. The response to acute signals and the result of manipulation of histone acetylation was consistent with the hypothesis that this refractory period corresponded to a phase of chromatin remodeling which significantly

  17. Academic Training: 2004 - 2005 ACADEMIC TRAINING PROGRAMME

    CERN Multimedia

    Françoise Benz

    2004-01-01

    1st Term - 01 October to 17 December 2004 REGULAR LECTURE PROGRAMME New Trends in Fusion Research by A. Fasoli, EPFL, Lausanne, CH 11, 12, 13 October Physics at e+e- linear collider by K. Desch, DESY, Hamburg, D 15, 16, 17, 18, 19 November LECTURE SERIES FOR POSTGRADUATE STUDENTS Standard Model by R. Barbieri, CERN-PH-TH 6, 7, 8, 9 10 December The lectures are open to all those interested, without application. The abstract of the lectures, as well as any change to the above information (title, dates, time, place etc) will be published in the CERN Bulletin, the WWW, and by notices before each term and for each series of lectures. ENSEIGNEMENT ACADEMIQUE ACADEMIC TRAINING Françoise Benz 73127 academic.training@cern.ch

  18. An overview on transcriptional regulators in Streptomyces.

    Science.gov (United States)

    Romero-Rodríguez, Alba; Robledo-Casados, Ivonne; Sánchez, Sergio

    2015-08-01

    Streptomyces are Gram-positive microorganisms able to adapt and respond to different environmental conditions. It is the largest genus of Actinobacteria comprising over 900 species. During their lifetime, these microorganisms are able to differentiate, produce aerial mycelia and secondary metabolites. All of these processes are controlled by subtle and precise regulatory systems. Regulation at the transcriptional initiation level is probably the most common for metabolic adaptation in bacteria. In this mechanism, the major players are proteins named transcription factors (TFs), capable of binding DNA in order to repress or activate the transcription of specific genes. Some of the TFs exert their action just like activators or repressors, whereas others can function in both manners, depending on the target promoter. Generally, TFs achieve their effects by using one- or two-component systems, linking a specific type of environmental stimulus to a transcriptional response. After DNA sequencing, many streptomycetes have been found to have chromosomes ranging between 6 and 12Mb in size, with high GC content (around 70%). They encode for approximately 7000 to 10,000 genes, 50 to 100 pseudogenes and a large set (around 12% of the total chromosome) of regulatory genes, organized in networks, controlling gene expression in these bacteria. Among the sequenced streptomycetes reported up to now, the number of transcription factors ranges from 471 to 1101. Among these, 315 to 691 correspond to transcriptional regulators and 31 to 76 are sigma factors. The aim of this work is to give a state of the art overview on transcription factors in the genus Streptomyces.

  19. Training brain networks and states.

    Science.gov (United States)

    Tang, Yi-Yuan; Posner, Michael I

    2014-07-01

    Brain training refers to practices that alter the brain in a way that improves cognition, and performance in domains beyond those involved in the training. We argue that brain training includes network training through repetitive practice that exercises specific brain networks and state training, which changes the brain state in a way that influences many networks. This opinion article considers two widely used methods - working memory training (WMT) and meditation training (MT) - to demonstrate the similarities and differences between network and state training. These two forms of training involve different areas of the brain and different forms of generalization. We propose a distinction between network and state training methods to improve understanding of the most effective brain training.

  20. Interactive Karyotyping Training

    Directory of Open Access Journals (Sweden)

    Ashwin Kotwaliwale

    2013-01-01

    Full Text Available Despite the wide use of newer techniques in genetic diagnostics, there remains a need for technologists to learn human chromosome morphology, identify abnormal metaphases and report clinical abnormalities. Global short age of cytogenetic trainers and a time consuming training process makes Karyotyping training difficult. We have developed a web based interactive Karyotyping training tool, KaryoTutor©, that allows technologists to learn karyotyping in an interactive environment and aids the trainer in the training process. KaryoTutor©provides visual clues for identifying abnormal chromosomes, provides instant test scores and includes a reference library of ideograms,sample chromosome images and reference materials. Trainees are able to recursively work on a case till a satisfactory result is achieved,with KaryoTutor providing interactive inputs.Additionally, trainers can assign cases and monitor trainee progress using audit trail management and other administrative features.

  1. Apprentices for training

    CERN Multimedia

    1992-01-01

    Every year CERN recruits and trains six technical and two administrative apprentices to the level of the Federal Certificate of Competence. This is performed in accordance with the laws and practice applicable in the Canton of Geneva.

  2. Plutonium Training Opportunities

    Energy Technology Data Exchange (ETDEWEB)

    Balatsky, Galya Ivanovna [Los Alamos National Lab. (LANL), Los Alamos, NM (United States); Wolkov, Benjamin [Los Alamos National Lab. (LANL), Los Alamos, NM (United States)

    2015-03-26

    This report was created to examine the current state of plutonium training in the United States and to discover ways in which to ensure that the next generation of plutonium workers are fully qualified.

  3. Does CRM training work?

    Science.gov (United States)

    Helmreich, R. L.

    1991-01-01

    Formal cockpit resource management training in crew coordination concepts increases the percentage of crews rated as above average in performance and decreases the percentage of crews rated as below average.

  4. Language Training: French

    CERN Multimedia

    Françoise Benz

    2005-01-01

    General and Professional French Courses The next session will take place from 24 January to 18 March 2005. These courses are open to all persons working on the CERN site, and to their spouses. For registration and further information on the courses, please consult our Web pages: http://www.cern.ch/Training/ or contact Mrs. Benz : Tel. 73127. Writing Professional Documents in French The next session will take place from 24 January to 18 March 2005. This course is designed for people with a good level of spoken French. Duration: 24 hours Price: 528 CHF (for 8 students) For further information and registration, please consult our Web pages: http://www.cern.ch/Training/ or contact Mrs. Benz : Tel. 73127. FORMATION EN LANGUES LANGUAGE TRAINING Françoise Benz 73127 language.training@cern.ch

  5. Language Training: French

    CERN Document Server

    Françoise Benz

    2005-01-01

    General and Professional French Courses The next session will take place from 18 April to 30 June 2005. These courses are open to all persons working on the CERN site, and to their spouses. For registration and further information on the courses, please consult our Web pages: http://cern.ch/Training or contact Mrs. Benz : Tel. 73127. Writing Professional Documents in French The next session will take place from 18 April to 30 June 2005. This course is designed for people with a good level of spoken French. Duration: 30 hours Price: 660 CHF (for 8 students) For further information and registration, please consult our Web pages: http://cern.ch/Training or contact Mrs. Benz : Tel. 73127. FORMATION EN LANGUES LANGUAGE TRAINING Françoise Benz 73127 language.training@cern.ch

  6. Language Training: French

    CERN Multimedia

    Françoise Benz

    2005-01-01

    General and Professional French Courses The next session will take place from 24 January to 18 March 2005. These courses are open to all persons working on the CERN site, and to their spouses. For registration and further information on the courses, please consult our Web pages: http://cern.ch/Training or contact Mrs. Benz : Tel. 73127. Writing Professional Documents in French The next session will take place from 24 January to 18 March 2005. This course is designed for people with a good level of spoken French. Duration: 24 hours Price: 528 CHF (for 8 students) For further information and registration, please consult our Web pages: http://cern.ch/Training or contact Mrs. Benz : Tel. 73127. FORMATION EN LANGUES LANGUAGE TRAINING Françoise Benz 73127 language.training@cern.ch

  7. The Last Horse Trains

    Institute of Scientific and Technical Information of China (English)

    2004-01-01

    Horse trains used to be the chief means of transport in southwest China's mountainous areas, areas that were almost inaccessible because of the difficult terrain. They have largely disappeared as most such areas are already serviced by modern road systems. At binzhonluo, however, the last horse trains can still be found, their drivers being Nus, Lisus and Tibetans. As I discovered at country fairs, goods shipped in by horse trains from Tibetan are mostly butter tea, edible fungus and wild orchid plants, and goods shipped to Tibet include drinks, batteries, salt and instant noodles. For centuries,horse trains have traversed the Nujiang Canyon or the Tibetan-Yi Corridor which, to be accurate, should be called an economic and cultural corridor linking Tibet and the rest of China.

  8. Boiler Operator Training.

    Science.gov (United States)

    Miller, Stanton S.

    1978-01-01

    This program, developed by the Nalco Chemical Company, helps with energy conservation in industrial plants. The program takes four to six weeks to complete. The training sessions last for about two hours. (BB)

  9. The Future Training Room.

    Science.gov (United States)

    Barbian, Jeff

    2001-01-01

    Looks at some of the electronic learning technology that has already been developed and will become common for training, including robots, lucid dreaming, tele-immersion, human interface technology, among others. (JOW)

  10. Identification of epididymis-specific transcripts in the mouse and rat by transcriptional profiling

    Institute of Scientific and Technical Information of China (English)

    Daniel S. Johnston; Terry T. Turner; Joshua N. Finger; Tracy L. Owtscharuk; S. Kopf; Scott A. Jelinsky

    2007-01-01

    As part of our efforts to identify novel contraceptive targets in the epididymis we performed transcriptional profiling on each of the 10 and 19 segments of the mouse and rat epididymidis, respectively, using Affymetrix whole genome microarrays. A total of 17 096 and 16 360 probe sets representing transcripts were identified as being expressed in the segmented mouse and rat epididymal transcriptomes, respectively. Comparison of the expressed murine transcripts against a mouse transcriptional profiling database derived from 22 other mouse tissues identified 77transcripts that were expressed uniquely in the epididymis. The expression of these genes was further evaluated by reverse transcription polymerase chain reaction (RT-PCR) analysis of RNA from 21 mouse tissues. RT-PCR analysis confirmed epididymis-specific expression of Defensin Beta 13 and identified two additional genes with expression restricted only to the epididymis and testis. Comparison of the 16 360 expressed transcripts in the rat epididymis with data of 21 other tissues from a rat transcriptional profiling database identified 110 transcripts specific for the epididymis.Sixty-two of these transcripts were further investigated by qPCR analysis. Only Defensin 22 (E3 epididymal protein)was shown to be completely specific for the epididymis. In addition, 14 transcripts showed more than 100-fold selective expression in the epididymis. The products of these genes might play important roles in epididymal and/or sperm function and further investigation and validation as contraceptive targets are warranted. The results of the studies described in this report are available at the Mammalian Reproductive Genetics (MRG) Database (http://mrg.genetics.washington.edu/).

  11. Altitude and endurance training.

    Science.gov (United States)

    Rusko, Heikki K; Tikkanen, Heikki O; Peltonen, Juha E

    2004-10-01

    The benefits of living and training at altitude (HiHi) for an improved altitude performance of athletes are clear, but controlled studies for an improved sea-level performance are controversial. The reasons for not having a positive effect of HiHi include: (1) the acclimatization effect may have been insufficient for elite athletes to stimulate an increase in red cell mass/haemoglobin mass because of too low an altitude (stress with possible overtraining symptoms and an increased frequency of infections. Moreover, the effects of hypoxia in the brain may influence both training intensity and physiological responses during training at altitude. Thus, interrupting hypoxic exposure by training in normoxia may be a key factor in avoiding or minimizing the noxious effects that are known to occur in chronic hypoxia. When comparing HiHi and HiLo (living high and training low), it is obvious that both can induce a positive acclimatization effect and increase the oxygen transport capacity of blood, at least in 'responders', if certain prerequisites are met. The minimum dose to attain a haematological acclimatization effect is > 12 h a day for at least 3 weeks at an altitude or simulated altitude of 2100-2500 m. Exposure to hypoxia appears to have some positive transfer effects on subsequent training in normoxia during and after HiLo. The increased oxygen transport capacity of blood allows training at higher intensity during and after HiLo in subsequent normoxia, thereby increasing the potential to improve some neuromuscular and cardiovascular determinants of endurance performance. The effects of hypoxic training and intermittent short-term severe hypoxia at rest are not yet clear and they require further study.

  12. National Biocontainment Training Center

    Science.gov (United States)

    2013-07-01

    Gina Papadakis ’ training included a review of the Galveston National Laboratory’s biosafety requirements for BSL2, 3 and 4 as well as BSL4 facility...training and BSL4 emergency procedures and incident response. Working with the NBTC’s expert staff, Drs. Druce and Papadakis spent time engaged in...Gina Papadakis , BSL4 Trainer Jet Newton, Dr. Julian Druce and GNL Director Dr. James LeDuc. Previous page right: The group is pictured with

  13. Effects of teacher training

    DEFF Research Database (Denmark)

    Wahlgren, Bjarne; Larsen, Lea Lund

    2010-01-01

    The article gives a short overview over existing knowledge concerning the effect of teacher training in relation to adult learning. It presents a research design for measuring the effect of teacher traning.......The article gives a short overview over existing knowledge concerning the effect of teacher training in relation to adult learning. It presents a research design for measuring the effect of teacher traning....

  14. Training cleanroom personnel.

    Science.gov (United States)

    Dixon, A M

    1991-01-01

    PEOPLE: the one resource that all companies have in common. Companies in general, and especially those involved in the clean or aseptic manufacturing environments cannot rely solely on technological innovation to increase quality and productivity. They must optimize their human assets to raise performance levels. Training is a critical factor. The training process is not simply to impart knowledge; but through education and involvement on a personal level, to alter behavior: CULTURE.

  15. Vocational Training Today: The Changing Relationship between Training and Employment.

    Science.gov (United States)

    Ducray, Gabriel

    1979-01-01

    Focusing on the changing relationship between training and employment, this article discusses (1) limitations of traditional vocational training policies, (2) the relationship between training and employment today, (3) changes in skills and the effects of structural factors on the employment situation, and (4) a modern vocational training policy.…

  16. Effects of Correspondence Training in an Abduction Prevention Training Program.

    Science.gov (United States)

    Olsen-Woods, Laurie A.; Miltenberger, Raymond G.; Foreman, Greg

    1998-01-01

    Examines the effects of adding correspondence training to a behavioral skills training package that taught abduction prevention skills to 31 children, ages 4-5 years. Results indicate that correspondence training did not improve correspondence between saying and doing target behaviors. However, both training approaches were equally effective in…

  17. Evolution of gastroenterology training.

    Science.gov (United States)

    Telleman, Hanna; Burger, Trevlyn Felicity; Mulder, Chris Jacob Johan

    2009-04-21

    There have been rapid developments in gastroenterology (GE) over the last decade. Up until the late 1980s, GE-training was incorporated in Internal Medicine training. The introduction of endoscopy has necessitated the need for additional training. Around the world different national boards have developed their own curricula which will be discussed in this paper. Emphasis will be placed on the curriculum recently introduced in The Netherlands. The internal medicine component has become a two-year requirement (Common Trunk) and the duration of training in GE has been extended to four years. Because of the growing complexity of GE, there are now four subspecialties: Interventional Endoscopy, Neuromotility, Oncology and Hepatology that trainees can choose from. These subspecialties each have predefined specific requirements. The World Gastroenterology Organization has drawn up a standard curriculum which can be of help to the boards in different countries. The curriculum emphasizes the knowledge and skill components. The curriculum also defines the training recommendations, the requirements of training facilities and competence evaluation of fellows and facilities, while less is said about research, finance and the number of gastroenterologists required. In the coming decades the curriculum will need to be revised continuously. Personalization of the curriculum will be the next challenge for the years to come.

  18. Monitoring spike train synchrony.

    Science.gov (United States)

    Kreuz, Thomas; Chicharro, Daniel; Houghton, Conor; Andrzejak, Ralph G; Mormann, Florian

    2013-03-01

    Recently, the SPIKE-distance has been proposed as a parameter-free and timescale-independent measure of spike train synchrony. This measure is time resolved since it relies on instantaneous estimates of spike train dissimilarity. However, its original definition led to spuriously high instantaneous values for eventlike firing patterns. Here we present a substantial improvement of this measure that eliminates this shortcoming. The reliability gained allows us to track changes in instantaneous clustering, i.e., time-localized patterns of (dis)similarity among multiple spike trains. Additional new features include selective and triggered temporal averaging as well as the instantaneous comparison of spike train groups. In a second step, a causal SPIKE-distance is defined such that the instantaneous values of dissimilarity rely on past information only so that time-resolved spike train synchrony can be estimated in real time. We demonstrate that these methods are capable of extracting valuable information from field data by monitoring the synchrony between neuronal spike trains during an epileptic seizure. Finally, the applicability of both the regular and the real-time SPIKE-distance to continuous data is illustrated on model electroencephalographic (EEG) recordings.

  19. Learning without training.

    Science.gov (United States)

    Beste, Christian; Dinse, Hubert R

    2013-06-03

    Achieving high-level skills is generally considered to require intense training, which is thought to optimally engage neuronal plasticity mechanisms. Recent work, however, suggests that intensive training may not be necessary for skill learning. Skills can be effectively acquired by a complementary approach in which the learning occurs in response to mere exposure to repetitive sensory stimulation. Such training-independent sensory learning induces lasting changes in perception and goal-directed behaviour in humans, without any explicit task training. We suggest that the effectiveness of this form of learning in different sensory domains stems from the fact that the stimulation protocols used are optimized to alter synaptic transmission and efficacy. While this approach directly links behavioural research in humans with studies on cellular plasticity, other approaches show that learning can occur even in the absence of an actual stimulus. These include learning through imagery or feedback-induced cortical activation, resulting in learning without task training. All these approaches challenge our understanding of the mechanisms that mediate learning. Apparently, humans can learn under conditions thought to be impossible a few years ago. Although the underlying mechanisms are far from being understood, training-independent sensory learning opens novel possibilities for applications aimed at augmenting human cognition.

  20. Evolution of gastroenterology training

    Institute of Scientific and Technical Information of China (English)

    Hanna Telleman; Trevlyn Felicity Burger; Chris Jacob Johan Mulder

    2009-01-01

    There have been rapid developments in gastroenterology (GE) over the last decade. Up until the late 1980s, GE-training was incorporated in Internal Medicine training. The introduction of endoscopy has necessitated the need for additional training. Around the world different national boards have developed their own curricula which will be discussed in this paper. Emphasis will be placed on the curriculum recently introduced in The Netherlands. The internal medicine component has become a two-year requirement (Common Trunk) and the duration of training in GE has been extended to four years. Because of the growing complexity of GE, there are now four subspecialties: Interventional Endoscopy, Neuromotility, Oncology and Hepatology that trainees can choose from. These subspecialties each have predefined specific requirements. The World Gastroenterology Organization has drawn up a standard curriculum which can be of help to the boards in different countries. The curriculum emphasizes the knowledge and skill components. The curriculum also defines the training recommendations, the requirements of training facilities and competence evaluation of fellows and facilities, while less is said about research, finance and the number of gastroenterologists required. In the coming decades the curriculum will need to be revised continuously. Personalization of the curriculum will be the next challenge for the years to come.

  1. Training induced cortical plasticity compared between three tongue training paradigms

    DEFF Research Database (Denmark)

    Kothari, Mohit; Svensson, Peter; Jensen, Jim

    2013-01-01

    The primary aim of this study was to investigate the effect of different training types and secondary to test gender differences on the training-related cortical plasticity induced by three different tongue training paradigms: 1. Therapeutic tongue exercises (TTE), 2. Playing computer games......) (control) were established using transcranial magnetic stimulation (TMS) at three time-points: (1) before tongue training, (2) immediately after training, (3) 1 h after training. Subject-based reports of motivation, fun, pain and fatigue were evaluated on 0-10 numerical rating scales (NRS) after training......-points. No significant effect of tongue training on FDI MEPs was observed (P>0.335). The tongue cortical motor map areas were not significantly increased by training (P>0.142). Training with TDS was most motivating and fun (Plevel was not different between groups...

  2. Annual training manual for security training: Protective force

    Energy Technology Data Exchange (ETDEWEB)

    1990-10-01

    Westinghouse is committed to high quality training relevant to the need of the Protective Forces at the Waste Isolation Pilot Plant (WIPP). The training programs at WIPP are designed to qualify Security personnel to perform WIPP security missions in a professional and responsible manner. The program consists of basic as well as sustainment training, as further described in this plan. This plan documents the WIPP Security training program for security personnel for calendar year 1990. The programs detailed in this plan are designed to adequately train persons to ensure the uninterrupted continuity of Department of Energy (DOE)/Westinghouse operations. The Security Training Program consists of four basic elements. These elements are (1) basic level training; (2) on-the-job training; (3) refresher training; and (4) in-service training.

  3. Structural analysis of nucleosomal barrier to transcription.

    Science.gov (United States)

    Gaykalova, Daria A; Kulaeva, Olga I; Volokh, Olesya; Shaytan, Alexey K; Hsieh, Fu-Kai; Kirpichnikov, Mikhail P; Sokolova, Olga S; Studitsky, Vasily M

    2015-10-27

    Thousands of human and Drosophila genes are regulated at the level of transcript elongation and nucleosomes are likely targets for this regulation. However, the molecular mechanisms of formation of the nucleosomal barrier to transcribing RNA polymerase II (Pol II) and nucleosome survival during/after transcription remain unknown. Here we show that both DNA-histone interactions and Pol II backtracking contribute to formation of the barrier and that nucleosome survival during transcription likely occurs through allosterically stabilized histone-histone interactions. Structural analysis indicates that after Pol II encounters the barrier, the enzyme backtracks and nucleosomal DNA recoils on the octamer, locking Pol II in the arrested state. DNA is displaced from one of the H2A/H2B dimers that remains associated with the octamer. The data reveal the importance of intranucleosomal DNA-protein and protein-protein interactions during conformational changes in the nucleosome structure on transcription. Mechanisms of nucleosomal barrier formation and nucleosome survival during transcription are proposed.

  4. Control and signal processing by transcriptional interference

    Science.gov (United States)

    Buetti-Dinh, Antoine; Ungricht, Rosemarie; Kelemen, János Z; Shetty, Chetak; Ratna, Prasuna; Becskei, Attila

    2009-01-01

    A transcriptional activator can suppress gene expression by interfering with transcription initiated by another activator. Transcriptional interference has been increasingly recognized as a regulatory mechanism of gene expression. The signals received by the two antagonistically acting activators are combined by the polymerase trafficking along the DNA. We have designed a dual-control genetic system in yeast to explore this antagonism systematically. Antagonism by an upstream activator bears the hallmarks of competitive inhibition, whereas a downstream activator inhibits gene expression non-competitively. When gene expression is induced weakly, the antagonistic activator can have a positive effect and can even trigger paradoxical activation. Equilibrium and non-equilibrium models of transcription shed light on the mechanism by which interference converts signals, and reveals that self-antagonism of activators imitates the behavior of feed-forward loops. Indeed, a synthetic circuit generates a bell-shaped response, so that the induction of expression is limited to a narrow range of the input signal. The identification of conserved regulatory principles of interference will help to predict the transcriptional response of genes in their genomic context. PMID:19690569

  5. Antioxidant supplementation does not alter endurance training adaptation

    DEFF Research Database (Denmark)

    Yfanti, Christina; Åkerström, Thorbjörn; Nielsen, Søren

    2010-01-01

    BACKGROUND: There is a considerable commercial market, especially within the sports community, claiming the need for antioxidant supplementation. One argument for antioxidant supplementation in sports is that physical exercise is associated with increased reactive oxygen and nitrogen species (RONS......) production, which may cause cell damage. However, RONS production may also activate redox sensitive signaling pathways and transcription factors, which subsequently may promote training adaptation. PURPOSE: Our aim was to investigate the effects of combined vitamin C and E supplementation to healthy...... individuals on different measures of exercise performance after endurance training. METHODS:: Using a double-blinded placebo-controlled design, moderately trained young men received either oral supplementation with vitamins C and E (n=11) or placebo (n=10) before and during 12 weeks of supervised, strenuous...

  6. Customer Satisfaction with Training Programs.

    Science.gov (United States)

    Mulder, Martin

    2001-01-01

    A model for evaluating customer satisfaction with training programs was tested with training purchasers. The model confirmed two types of projects: training aimed at achieving learning results and at changing job performance. The model did not fit for training intended to support organizational change. (Contains 31 references.) (SK)

  7. Aircraft Simulators and Pilot Training.

    Science.gov (United States)

    Caro, Paul W.

    Flight simulators are built as realistically as possible, presumably to enhance their training value. Yet, their training value is determined by the way they are used. Traditionally, simulators have been less important for training than have aircraft, but they are currently emerging as primary pilot training vehicles. This new emphasis is an…

  8. Sustainable Library Development Training Package

    Science.gov (United States)

    Peace Corps, 2012

    2012-01-01

    This Sustainable Library Development Training Package supports Peace Corps' Focus In/Train Up strategy, which was implemented following the 2010 Comprehensive Agency Assessment. Sustainable Library Development is a technical training package in Peace Corps programming within the Education sector. The training package addresses the Volunteer…

  9. Controllability analysis of transcriptional regulatory networks reveals circular control patterns among transcription factors

    DEFF Research Database (Denmark)

    Österlund, Tobias; Bordel, Sergio; Nielsen, Jens

    2015-01-01

    Transcriptional regulation is the most committed type of regulation in living cells where transcription factors (TFs) control the expression of their target genes and TF expression is controlled by other TFs forming complex transcriptional regulatory networks that can be highly interconnected. Here...... we analyze the topology and organization of nine transcriptional regulatory networks for E. coli, yeast, mouse and human, and we evaluate how the structure of these networks influences two of their key properties, namely controllability and stability. We calculate the controllability for each network...... as a measure of the organization and interconnectivity of the network. We find that the number of driver nodes n(D) needed to control the whole network is 64% of the TFs in the E. coli transcriptional regulatory network in contrast to only 17% for the yeast network, 4% for the mouse network and 8...

  10. Transcription regulatory networks analysis using CAGE

    KAUST Repository

    Tegnér, Jesper N.

    2009-10-01

    Mapping out cellular networks in general and transcriptional networks in particular has proved to be a bottle-neck hampering our understanding of biological processes. Integrative approaches fusing computational and experimental technologies for decoding transcriptional networks at a high level of resolution is therefore of uttermost importance. Yet, this is challenging since the control of gene expression in eukaryotes is a complex multi-level process influenced by several epigenetic factors and the fine interplay between regulatory proteins and the promoter structure governing the combinatorial regulation of gene expression. In this chapter we review how the CAGE data can be integrated with other measurements such as expression, physical interactions and computational prediction of regulatory motifs, which together can provide a genome-wide picture of eukaryotic transcriptional regulatory networks at a new level of resolution. © 2010 by Pan Stanford Publishing Pte. Ltd. All rights reserved.

  11. Transcription and the Pitch Angle of DNA

    CERN Document Server

    Olsen, Kasper W

    2013-01-01

    The question of the value of the pitch angle of DNA is visited from the perspective of a geometrical analysis of transcription. It is suggested that for transcription to be possible, the pitch angle of B-DNA must be smaller than the angle of zero-twist. At the zero-twist angle the double helix is maximally rotated and its strain-twist coupling vanishes. A numerical estimate of the pitch angle for B-DNA based on differential geometry is compared with numbers obtained from existing empirical data. The crystallographic studies shows that the pitch angle is approximately 38 deg., less than the corresponding zero-twist angle of 41.8 deg., which is consistent with the suggested principle for transcription.

  12. Transcription factor CTCF and mammalian genome organization

    Directory of Open Access Journals (Sweden)

    Kotova E. S.

    2014-07-01

    Full Text Available The CTCF transcription factor is thought to be one of the main participants in various gene regulatory networks including transcription activation and repression, formation of independently functioning chromatin domains, regulation of imprinting etc. Sequencing of human and other genomes opened up a possibility to ascertain the genomic distribution of CTCF binding sites and to identify CTCF-dependent cis-regulatory elements, including insulators. In the review, we summarized recent data on CTCF functioning within a framework of the chromatin loop domain hypothesis of large-scale regulation of the genome activity. Its fundamental properties allow CTCF to serve as a transcription factor, an insulator protein and a dispersed genome-wide demarcation tool able to recruit various factors that emerge in response to diverse external and internal signals, and thus to exert its signal-specific function(s.

  13. Runx transcription factors in neuronal development

    Directory of Open Access Journals (Sweden)

    Shiga Takashi

    2008-08-01

    Full Text Available Abstract Runt-related (Runx transcription factors control diverse aspects of embryonic development and are responsible for the pathogenesis of many human diseases. In recent years, the functions of this transcription factor family in the nervous system have just begun to be understood. In dorsal root ganglion neurons, Runx1 and Runx3 play pivotal roles in the development of nociceptive and proprioceptive sensory neurons, respectively. Runx appears to control the transcriptional regulation of neurotrophin receptors, numerous ion channels and neuropeptides. As a consequence, Runx contributes to diverse aspects of the sensory system in higher vertebrates. In this review, we summarize recent progress in determining the role of Runx in neuronal development.

  14. Switching on cilia: transcriptional networks regulating ciliogenesis.

    Science.gov (United States)

    Choksi, Semil P; Lauter, Gilbert; Swoboda, Peter; Roy, Sudipto

    2014-04-01

    Cilia play many essential roles in fluid transport and cellular locomotion, and as sensory hubs for a variety of signal transduction pathways. Despite having a conserved basic morphology, cilia vary extensively in their shapes and sizes, ultrastructural details, numbers per cell, motility patterns and sensory capabilities. Emerging evidence indicates that this diversity, which is intimately linked to the different functions that cilia perform, is in large part programmed at the transcriptional level. Here, we review our understanding of the transcriptional control of ciliary biogenesis, highlighting the activities of FOXJ1 and the RFX family of transcriptional regulators. In addition, we examine how a number of signaling pathways, and lineage and cell fate determinants can induce and modulate ciliogenic programs to bring about the differentiation of distinct cilia types.

  15. Battles and hijacks: noncoding transcription in plants.

    Science.gov (United States)

    Ariel, Federico; Romero-Barrios, Natali; Jégu, Teddy; Benhamed, Moussa; Crespi, Martin

    2015-06-01

    Noncoding RNAs have emerged as major components of the eukaryotic transcriptome. Genome-wide analyses revealed the existence of thousands of long noncoding RNAs (lncRNAs) in several plant species. Plant lncRNAs are transcribed by the plant-specific RNA polymerases Pol IV and Pol V, leading to transcriptional gene silencing, as well as by Pol II. They are involved in a wide range of regulatory mechanisms impacting on gene expression, including chromatin remodeling, modulation of alternative splicing, fine-tuning of miRNA activity, and the control of mRNA translation or accumulation. Recently, dual noncoding transcription by alternative RNA polymerases was implicated in epigenetic and chromatin conformation dynamics. This review integrates the current knowledge on the regulatory mechanisms acting through plant noncoding transcription.

  16. Automatic Phonetic Transcription for Danish Speech Recognition

    DEFF Research Database (Denmark)

    Kirkedal, Andreas Søeborg

    Automatic speech recognition (ASR) uses dictionaries that map orthographic words to their phonetic representation. To minimize the occurrence of out-of-vocabulary words, ASR requires large phonetic dictionaries to model pronunciation. Hand-crafted high-quality phonetic dictionaries are difficult...... of automatic phonetic transcriptions vary greatly with respect to language and transcription strategy. For some languages where the difference between the graphemic and phonetic representations are small, graphemic transcriptions can be used to create ASR systems with acceptable performance. In other languages......, like Danish, the graphemic and phonetic representations are very dissimilar and more complex rewriting rules must be applied to create the correct phonetic representation. Automatic phonetic transcribers use different strategies, from deep analysis to shallow rewriting rules, to produce phonetic...

  17. Battles and hijacks: Noncoding transcription in plants

    KAUST Repository

    Ariel, Federico

    2015-06-01

    Noncoding RNAs have emerged as major components of the eukaryotic transcriptome. Genome-wide analyses revealed the existence of thousands of long noncoding RNAs (lncRNAs) in several plant species. Plant lncRNAs are transcribed by the plant-specific RNA polymerases Pol IV and Pol V, leading to transcriptional gene silencing, as well as by Pol II. They are involved in a wide range of regulatory mechanisms impacting on gene expression, including chromatin remodeling, modulation of alternative splicing, fine-tuning of miRNA activity, and the control of mRNA translation or accumulation. Recently, dual noncoding transcription by alternative RNA polymerases was implicated in epigenetic and chromatin conformation dynamics. This review integrates the current knowledge on the regulatory mechanisms acting through plant noncoding transcription. © 2015 Elsevier Ltd.

  18. Deciphering the Innate Lymphoid Cell Transcriptional Program

    Directory of Open Access Journals (Sweden)

    Cyril Seillet

    2016-10-01

    Full Text Available Innate lymphoid cells (ILCs are enriched at mucosal surfaces, where they provide immune surveillance. All ILC subsets develop from a common progenitor that gives rise to pre-committed progenitors for each of the ILC lineages. Currently, the temporal control of gene expression that guides the emergence of these progenitors is poorly understood. We used global transcriptional mapping to analyze gene expression in different ILC progenitors. We identified PD-1 to be specifically expressed in PLZF+ ILCp and revealed that the timing and order of expression of the transcription factors NFIL3, ID2, and TCF-1 was critical. Importantly, induction of ILC lineage commitment required only transient expression of NFIL3 prior to ID2 and TCF-1 expression. These findings highlight the importance of the temporal program that permits commitment of progenitors to the ILC lineage, and they expand our understanding of the core transcriptional program by identifying potential regulators of ILC development.

  19. Transcriptional network underlying Caenorhabditis elegans vulval development.

    Science.gov (United States)

    Inoue, Takao; Wang, Minqin; Ririe, Ted O; Fernandes, Jolene S; Sternberg, Paul W

    2005-04-05

    The vulval development of Caenorhabditis elegans provides an opportunity to investigate genetic networks that control gene expression during organogenesis. During the fourth larval stage (L4), seven vulval cell types are produced, each of which executes a distinct gene expression program. We analyze how the expression of cell-type-specific genes is regulated. Ras and Wnt signaling pathways play major roles in generating the spatial pattern of cell types and regulate gene expression through a network of transcription factors. One transcription factor (lin-29) primarily controls the temporal expression pattern. Other transcription factors (lin-11, cog-1, and egl-38) act in combination to control cell-type-specific gene expression. The complexity of the network arises in part because of the dynamic nature of gene expression, in part because of the presence of seven cell types, and also because there are multiple regulatory paths for gene expression within each cell type.

  20. Conservation and implications of eukaryote transcriptional regulatory regions across multiple species

    Directory of Open Access Journals (Sweden)

    Deng Minghua

    2008-12-01

    Full Text Available Abstract Background Increasing evidence shows that whole genomes of eukaryotes are almost entirely transcribed into both protein coding genes and an enormous number of non-protein-coding RNAs (ncRNAs. Therefore, revealing the underlying regulatory mechanisms of transcripts becomes imperative. However, for a complete understanding of transcriptional regulatory mechanisms, we need to identify the regions in which they are found. We will call these transcriptional regulation regions, or TRRs, which can be considered functional regions containing a cluster of regulatory elements that cooperatively recruit transcriptional factors for binding and then regulating the expression of transcripts. Results We constructed a hierarchical stochastic language (HSL model for the identification of core TRRs in yeast based on regulatory cooperation among TRR elements. The HSL model trained based on yeast achieved comparable accuracy in predicting TRRs in other species, e.g., fruit fly, human, and rice, thus demonstrating the conservation of TRRs across species. The HSL model was also used to identify the TRRs of genes, such as p53 or OsALYL1, as well as microRNAs. In addition, the ENCODE regions were examined by HSL, and TRRs were found to pervasively locate in the genomes. Conclusion Our findings indicate that 1 the HSL model can be used to accurately predict core TRRs of transcripts across species and 2 identified core TRRs by HSL are proper candidates for the further scrutiny of specific regulatory elements and mechanisms. Meanwhile, the regulatory activity taking place in the abundant numbers of ncRNAs might account for the ubiquitous presence of TRRs across the genome. In addition, we also found that the TRRs of protein coding genes and ncRNAs are similar in structure, with the latter being more conserved than the former.

  1. PhyloScan: identification of transcription factor binding sites using cross-species evidence

    Directory of Open Access Journals (Sweden)

    Newberg Lee A

    2007-01-01

    Full Text Available Abstract Background When transcription factor binding sites are known for a particular transcription factor, it is possible to construct a motif model that can be used to scan sequences for additional sites. However, few statistically significant sites are revealed when a transcription factor binding site motif model is used to scan a genome-scale database. Methods We have developed a scanning algorithm, PhyloScan, which combines evidence from matching sites found in orthologous data from several related species with evidence from multiple sites within an intergenic region, to better detect regulons. The orthologous sequence data may be multiply aligned, unaligned, or a combination of aligned and unaligned. In aligned data, PhyloScan statistically accounts for the phylogenetic dependence of the species contributing data to the alignment and, in unaligned data, the evidence for sites is combined assuming phylogenetic independence of the species. The statistical significance of the gene predictions is calculated directly, without employing training sets. Results In a test of our methodology on synthetic data modeled on seven Enterobacteriales, four Vibrionales, and three Pasteurellales species, PhyloScan produces better sensitivity and specificity than MONKEY, an advanced scanning approach that also searches a genome for transcription factor binding sites using phylogenetic information. The application of the algorithm to real sequence data from seven Enterobacteriales species identifies novel Crp and PurR transcription factor binding sites, thus providing several new potential sites for these transcription factors. These sites enable targeted experimental validation and thus further delineation of the Crp and PurR regulons in E. coli. Conclusion Better sensitivity and specificity can be achieved through a combination of (1 using mixed alignable and non-alignable sequence data and (2 combining evidence from multiple sites within an intergenic

  2. IKAROS: a multifunctional regulator of the polymerase II transcription cycle.

    Science.gov (United States)

    Bottardi, Stefania; Mavoungou, Lionel; Milot, Eric

    2015-09-01

    Transcription factors are important determinants of lineage specification during hematopoiesis. They favor recruitment of cofactors involved in epigenetic regulation, thereby defining patterns of gene expression in a development- and lineage-specific manner. Additionally, transcription factors can facilitate transcription preinitiation complex (PIC) formation and assembly on chromatin. Interestingly, a few lineage-specific transcription factors, including IKAROS, also regulate transcription elongation. IKAROS is a tumor suppressor frequently inactivated in leukemia and associated with a poor prognosis. It forms a complex with the nucleosome remodeling and deacetylase (NuRD) complex and the positive transcription elongation factor b (P-TEFb), which is required for productive transcription elongation. It has also been reported that IKAROS interacts with factors involved in transcription termination. Here we review these and other recent findings that establish IKAROS as the first transcription factor found to act as a multifunctional regulator of the transcription cycle in hematopoietic cells.

  3. Direct competition assay for transcription fidelity.

    Science.gov (United States)

    Lubkowska, Lucyna; Kireeva, Maria L

    2015-01-01

    Accurate transcription is essential for faithful information flow from DNA to RNA and to the protein. Mechanisms of cognate substrate selection by RNA polymerases are currently elucidated by structural, genetic, and biochemical approaches. Here, we describe a fast and reliable approach to quantitative analyses of transcription fidelity, applicable to analyses of RNA polymerase selectivity against misincorporation, incorporation of dNMPs, and chemically modified rNMP analogues. The method is based on different electrophoretic mobility of RNA oligomers of the same length but differing in sequence.

  4. Transcriptional networks leading to symbiotic nodule organogenesis.

    Science.gov (United States)

    Soyano, Takashi; Hayashi, Makoto

    2014-08-01

    The symbiosis with nitrogen-fixing bacteria leading to root nodules is a relatively recent evolutionary innovation and limited to a distinct order of land plants. It has long been a mystery how plants have invented this complex trait. However, recent advances in molecular genetics of model legumes has elucidated genes involved in the development of root nodules, providing insights into this process. Here we discuss how the de novo assembly of transcriptional networks may account for the predisposition to nodulate. Transcriptional networks and modes of gene regulation from the arbuscular mycorrhizal symbiosis, nitrate responses and aspects of lateral root development have likely all contributed to the emergence and development of root nodules.

  5. Angiotensinogen Gene Transcription in Pulmonary Fibrosis

    Science.gov (United States)

    Uhal, Bruce D.; Dang, My-Trang T.; Li, Xiaopeng; Abdul-Hafez, Amal

    2012-01-01

    An established body of literature supports the hypothesis that activation of a local tissue angiotensin (ANG) system in the extravascular tissue compartment of the lungs is required for lung fibrogenesis. Transcriptional activation of the angiotensinogen (AGT) gene is believed to be a critical and necessary step in this activation. This paper summarizes the data in support of this theory and discusses transcriptional regulation of AGT, with an emphasis on lung AGT synthesis as a determinant of fibrosis severity. Genetic data linking AGT polymorphisms to the severity of disease in Idiopathic Pulmonary Fibrosis are also discussed. PMID:22500179

  6. Angiotensinogen Gene Transcription in Pulmonary Fibrosis

    Directory of Open Access Journals (Sweden)

    Bruce D. Uhal

    2012-01-01

    Full Text Available An established body of literature supports the hypothesis that activation of a local tissue angiotensin (ANG system in the extravascular tissue compartment of the lungs is required for lung fibrogenesis. Transcriptional activation of the angiotensinogen (AGT gene is believed to be a critical and necessary step in this activation. This paper summarizes the data in support of this theory and discusses transcriptional regulation of AGT, with an emphasis on lung AGT synthesis as a determinant of fibrosis severity. Genetic data linking AGT polymorphisms to the severity of disease in Idiopathic Pulmonary Fibrosis are also discussed.

  7. The retinoblastoma protein as a transcriptional repressor

    DEFF Research Database (Denmark)

    Helin, K; Ed, H

    1993-01-01

    The retinoblastoma protein (pRB) is one of the best-studied tumour suppressor gene products. Its loss during the genesis of many human tumours, its inactivation by several DNA tumour virus oncoproteins, and its ability to inhibit cell growth when introduced into dividing cells all suggest that pRB...... negatively regulates some aspect of normal cell growth. The discovery that pRB associates with transcription factors such as E2F has provided the first model for pRB function. In this review, we discuss how pRB may regulate cell growth by repressing transcription of genes essential for cell proliferation....

  8. Trainee Readiness For Diversity Training

    Directory of Open Access Journals (Sweden)

    Yunhyung Chung

    2013-11-01

    Full Text Available Although trainee readiness is critical for diversity training effectiveness, extant research has not paid attention to the relationship between trainee readiness for diversity training and diversity training outcomes. This study identifies motivational, behavioral, and cognitive trainee readiness for diversity training and proposes a theoretical framework of how individual characteristics (perceived discrimination, demographic attributes, and previous diversity-related experience and organizational characteristics (diversity climate and demographic dissimilarity influence motivational, behavioral, and cognitive trainee readiness for diversity training.

  9. Identifying discharge practice training needs.

    Science.gov (United States)

    Lees, L; Emmerson, K

    A training needs analysis tool was developed to identify nurses' discharge training needs and to improve discharge practice. The tool includes 49 elements of discharge practice subdivided into four areas: corporate, operational, clinical and nurse-led discharge. The tool was disseminated to 15 wards on two hospital sites with assistance from the practice development team. Analysis of discharge training is important to assess discharge training needs and to identify staff who may assist with training.

  10. 38 CFR 21.4265 - Practical training approved as institutional training or on-job training.

    Science.gov (United States)

    2010-07-01

    ...) The clinical training is; (a) An integral part of the course; (b) A prerequisite to the successful... 38 Pensions, Bonuses, and Veterans' Relief 2 2010-07-01 2010-07-01 false Practical training approved as institutional training or on-job training. 21.4265 Section 21.4265 Pensions, Bonuses,...

  11. TECHNICAL TRAINING: Places available**

    CERN Multimedia

    2003-01-01

    If you wish to participate in one of the following courses, please discuss with your supervisor and apply electronically directly from the course description pages that can be found on the Web at: http://www.cern.ch/Training/ or fill in an "application for training" form available from your Divisional Secretariat or from your DTO (Divisional Training Officer). Applications will be accepted in the order of their receipt. TECHNICAL TRAINING Monique Duval tel. 74924 technical.training@cern.ch ** The number of places available may vary. Please check our Web site to find out the current availability. Places are available in the following courses: Hands-on Introduction to Python Programming : 12 - 14.11.03(3 days) ACCESS 2000 - niveau 1 : 13 & 14.11.03 (2 jours) C++ for Particle Physicists : 17 - 21.11.03 (6 X 3-hour lectures) Programmation automate Schneider TSX Premium - niveau 2 : 18 - 21.11.03 (4 jours) Project Planning with MS-Project  (free of charg...

  12. TECHNICAL TRAINING: Places available**

    CERN Multimedia

    2003-01-01

    If you wish to participate in one of the following courses, please discuss with your supervisor and apply electronically directly from the course description pages that can be found on the Web at: http://www.cern.ch/Training/ or fill in an "application for training" form available from your Divisional Secretariat or from your DTO (Divisional Training Officer). Applications will be accepted in the order of their receipt. TECHNICAL TRAINING Monique Duval Tel. 74924 technical.training@cern.ch ** The number of places available may vary. Please check our Web site to find out the current availability. Places are available in the following courses: The EDMS-MTF in practice (free of charge) : 28 -  30.10.03 (6 half-day sessions) AutoCAD 2002 – Level 1 : 3, 4, 12, 13.11.03 (4 days) LabVIEW TestStand ver. 3 : 4 & 5.11.03 (2 days) Introduction to PSpice : 4.11.03 p.m. (half-day) Hands-on Introduction to Python Programming : 12 – 14.11.03 (3 days) ACCESS ...

  13. Technical Training: Places available

    CERN Multimedia

    2004-01-01

    If you wish to participate in one of the following courses, please discuss with your supervisor and apply electronically directly from the course description pages that can be found on the Web at: http://www.cern.ch/Training/ or fill in an "application for training" form available from your Divisional Secretariat or from your DTO (Divisional Training Officer). Applications will be accepted in the order of their receipt. TECHNICAL TRAINING Monique Duval tel. 74924 technical.training@cern.ch The number of places available may vary. Please check our Web site to find out the current availability. Places are available in the following courses: Computational Electromagnetics with the ELEKTRA Module of OPERA-3D : 27 & 28.4.2004 (2 days) Hands-on Introduction to Python Programming : 3 - 5.5.2004 (3 days) LabVIEW Base 2 : 6 & 7.5.2004 (2 jours) Project Planning with MS-Project : 6 & 13.5.2004 (2 days) Word 2003 - niveau 1 : 10 & 11.5.2004 (2 jours) Oracle 9i : SQL : 10 - 12.5.2004 (3...

  14. Technical Training: Places available

    CERN Multimedia

    2004-01-01

    If you wish to participate in one of the following courses, please discuss with your supervisor and apply electronically directly from the course description pages that can be found on the Web at: http://www.cern.ch/Training/ or fill in an "application for training" form available from your Divisional Secretariat or from your DTO (Divisional Training Officer). Applications will be accepted in the order of their receipt. TECHNICAL TRAINING Monique Duval tel. 74924 technical.training@cern.ch The number of places available may vary. Please check our Web site to find out the current availability. Places are available in the following courses: FrontPage XP - niveau 2 : 26 & 27.4.2004 (2 jours) The Joint PVSS JCOP Framework : 26 - 30.4.2004 (5 days) Computational Electromagnetics with the ELEKTRA Module of OPERA-3D : 27 & 28.4.2004 (2 days) Hands-on Introduction to Python Programming : 3 - 5.5.2004 (3 days) LabVIEW Base 2 : 6 & 7.5.2004 (2 jours) Project Pla...

  15. Technical Training: Places available

    CERN Multimedia

    2004-01-01

    If you wish to participate in one of the following courses, please discuss with your supervisor and apply electronically directly from the course description pages that can be found on the Web at: http://www.cern.ch/Training/ or fill in an "application for training" form available from your Divisional Secretariat or from your DTO (Divisional Training Officer). Applications will be accepted in the order of their receipt. TECHNICAL TRAINING Monique Duval tel. 74924 technical.training@cern.ch The number of places available may vary. Please check our Web site to find out the current availability. Places are available in the following courses: AutoCAD 2002 - niveau 1 : 19, 20.4 et 3, 4.5.2004 (4 jours) Oracle 8i/9i - Develop Web-based Applications with PL/SQL : 19 & 20.4.2004 (2 days) Introduction to ANSYS : 20 - 23.4.2004 (4 days) LabVIEW Hands-on : 20.4.2004 (half-day, p.m.) FrontPage XP - niveau 2 : 26 & 27.4.2004 (2 jours) The Joint PVSS JCOP Framework : 26 -...

  16. Ethics Training in Psychiatry

    Directory of Open Access Journals (Sweden)

    Sinan Guloksuz

    2009-09-01

    Full Text Available Although ethics training is one of the core components of psychiatric education, it is not sufficiently addressed in the curricula of many educational institutions. It is shown that many of the psychiatry residents received no ethics training in both residency and medical school. Predictably, over half of the psychiatry residents had faced an ethical dilemma that they felt unprepared to meet, and nearly all of them indicated ethics education would have helped them to solve this dilemma. In addition to learning about the fundamental topics of ethics like confidentiality, boundary violations, justice, benefience and nonmaleficence, psychiatrists must also learn to deal with other hidden ethical dilemmas which are mostly due to the changing world order. It is obvious that residency training should include a well developed ethics curriculum. However, some still believe that ethical principles cannot be taught and are formed in one’s early moral development. Accepting the fact that teaching ethics is difficult, we believe that it is getting easier with the new methods for teaching in medicine. These methods are clinical supervisions, rol-models, case studies, role playing, small group discussions, team based learning and “let’s talking medicine” groups which is a useful methods for discussing ethics dilemmas on daily practice and C.A.R.E (Core Beliefs, Actions, Reasons, Experience which is a special training method for teaching ethics. In this review, the need of ethics training in residency curriculum will be discussed and new methods for teaching ethics will be proposed.

  17. Technical Training: Places Available

    CERN Multimedia

    Monique Duval

    2004-01-01

    If you wish to participate in one of the following courses, please discuss with your supervisor and apply electronically directly from the course description pages that can be found on the Web at: http://www.cern.ch/Training/ or fill in an 'application for training' form available from your Divisional Secretariat or from your DTO (Divisional Training Officer). Applications will be accepted in the order of their receipt. Technical Training Monique Duval - Tel.74924 technical.training@cern.ch The number of places available may vary. Please check our Web site to find out the current availability. Places are available in the following courses: Oracle 9i: SQL: 17 - 19.5.2004 (3 days) Word 2003 - niveau 2 : 24 & 25.5.2004 (2 jours) EXCEL 2003 - niveau 1 : 27 & 28.5.2004 (2 jours) STEP7 Programming Level 1: 1 - 4.6.2004 (4 days) Oracle 9i : Programming with PL/SQL: 2 - 4.6.2004 (3 days) CST Microwave Studio: 3 & 4.6.2004 (2 days) Oracle 9i : New f...

  18. Technical Training: Places available

    CERN Multimedia

    Monique Duval

    2004-01-01

    If you wish to participate in one of the following courses, please discuss with your supervisor and apply electronically directly from the course description pages that can be found on the Web at: http://www.cern.ch/Training/ or fill in an 'application for training' form available from your Divisional Secretariat or from your DTO (Divisional Training Officer). Applications will be accepted in the order of their receipt. Technical Training Monique Duval - Tel.74924 technical.training@cern.ch The number of places available may vary. Please check our Web site to find out the current availability. Places are available in the following courses: Word 2003 - niveau 2 : 24 & 25.5.2004 (2 jours) VisualEliteHDL : 25 & 26.5.2004 (2 days) EXCEL 2003 - niveau 1 : 27 & 28.5.2004 (2 jours) STEP7 Programming Level 1: 1 - 4.6.2004 (4 days) Oracle 9i : Programming with PL/SQL: 2 - 4.6.2004 (3 days) CST Microwave Studio: 3 & 4.6.2004 (2 days) Oracle 9...

  19. Technical Training: Places available

    CERN Multimedia

    2004-01-01

    If you wish to participate in one of the following courses, please discuss with your supervisor and apply electronically directly from the course description pages that can be found on the Web at: http://www.cern.ch/Training/ or fill in an "application for training" form available from your Divisional Secretariat or from your DTO (Divisional Training Officer). Applications will be accepted in the order of their receipt. TECHNICAL TRAINING Monique Duval tel. 74924 technical.training@cern.ch The number of places available may vary. Please check our Web site to find out the current availability. Places are available in the following courses: Project Planning with MS-Project :6 & 13.5.2004 (2 days) Word 2003 - niveau 1 : 10 & 11.5.2004 (2 jours) Oracle 9i : SQL : 17 - 19.5.2004 (3 days) Word 2003 - niveau 2 : 24 & 25.5.2004 (2 jours) EXCEL 2003 - niveau 1: 27 & 28.5.2004 (2 jours) STEP7 Programming Level 1 : 1 - 4.6.2004 (4 days) Oracle 9i : Programming with PL/SQL : 2 - 4.6.2...

  20. Technical Training: Places available

    CERN Multimedia

    2004-01-01

    If you wish to participate in one of the following courses, please discuss with your supervisor and apply electronically directly from the course description pages that can be found on the Web at: http://www.cern.ch/Training/ or fill in an "application for training" form available from your Divisional Secretariat or from your DTO (Divisional Training Officer). Applications will be accepted in the order of their receipt. TECHNICAL TRAINING Monique Duval tel. 74924 technical.training@cern.ch The number of places available may vary. Please check our Web site to find out the current availability. Places are available in the following courses: Instructor-led WBTechT study or follow-up for Microsoft applications : 1.4.2004 (morning) FrontPage XP - niveau 1 : 5 & 6.4.2004 (2 jours) AutoCAD 2002 - niveau 1 : 19, 20.4 et 3, 4.5.2004 (4 jours) Oracle 8i/9i - Develop Web-based Applications with PL/SQL : 19 & 20.4.2004 (2 days) Introduction to ANSYS : 20 - 23.4.2004 (4 day...

  1. Technical Training: Places available

    CERN Multimedia

    2004-01-01

    If you wish to participate in one of the following courses, please discuss with your supervisor and apply electronically directly from the course description pages that can be found on the Web at: http://www.cern.ch/Training/ or fill in an "application for training" form available from your Divisional Secretariat or from your DTO (Divisional Training Officer). Applications will be accepted in the order of their receipt. TECHNICAL TRAINING Monique Duval tel. 74924 technical.training@cern.ch The number of places available may vary. Please check our Web site to find out the current availability. Places are available in the following courses: Instructor-led WBTechT study or follow-up for Microsoft applications : 19.2.2004 (morning) LabVIEW TestStand I (E) : 23 & 24.2.2004 (2 days) LabVIEW base 1 : 25 - 27.2.2004 (3 jours) Instructor-led WBTechT study or follow-up for Microsoft applications : 26.2.2004 (morning) CLEAN-2002 : Working in a Cleanroom : 10.3.2004 (afternoon - free of charge) C++ for Pa...

  2. Technical Training: Places available

    CERN Multimedia

    2004-01-01

    If you wish to participate in one of the following courses, please discuss with your supervisor and apply electronically directly from the course description pages that can be found on the Web at: http://www.cern.ch/Training/ or fill in an "application for training" form available from your Divisional Secretariat or from your DTO (Divisional Training Officer). Applications will be accepted in the order of their receipt. TECHNICAL TRAINING Monique Duval tel. 74924 technical.training@cern.ch The number of places available may vary. Please check our Web site to find out the current availability. Places are available in the following courses: Instructor-led WBTechT study for Microsoft applications :12.2.2004 (morning) Instructor-led WBTechT study or follow-up for Microsoft applications : 19.2.2004 (morning) LabVIEW TestStand I (E) : 23 & 24.2.2004 (2 days) LabVIEW base 1 : 25 - 27.2.2004 (3 jours) Instructor-led WBTechT study or follow-up for Microsoft applications : 19.2.2004 (morning) CLEAN-2002 ...

  3. Technical Training: Places available

    CERN Multimedia

    2004-01-01

    If you wish to participate in one of the following courses, please discuss with your supervisor and apply electronically directly from the course description pages that can be found on the Web at: http://www.cern.ch/Training/ or fill in an "application for training" form available from your Divisional Secretariat or from your DTO (Divisional Training Officer). Applications will be accepted in the order of their receipt. TECHNICAL TRAINING Monique Duval tel. 74924 technical.training@cern.ch The number of places available may vary. Please check our Web site to find out the current availability. Places are available in the following courses: LabVIEW base 1 : 25 - 27.2.2004(3 jours) Instructor-led WBTechT study or follow-up for Microsoft applications : 26.2.2004 (morning) CLEAN-2002 : Working in a Cleanroom : 10.3.2004 (afternoon - free of charge) C++ for Particle Physicists : 8 - 12.3.2004 (6 X 4-hour sessions) LabVIEW hands-on (E) : 16.3.2004 (afternoon) LabVIEW Basics 1 : 22 - 24.3.2004 ...

  4. Technical Training: Places available

    CERN Multimedia

    Monique Duval

    2004-01-01

    If you wish to participate in one of the following courses, please discuss with your supervisor and apply electronically directly from the course description pages that can be found on the Web at: http://www.cern.ch/Training/ or fill in an "application for training" form available from your Divisional Secretariat or from your DTO (Divisional Training Officer). Applications will be accepted in the order of their receipt. TECHNICAL TRAINING Monique Duval tel. 74924 technical.training@cern.ch The number of places available may vary. Please check our Web site to find out the current availability. Places are available in the following courses: C++ for Particle Physicists : 8 - 12.3.2004 (6 X 4-hour sessions) Introduction to the CERN EDMS : 9.3.2004 (1 day, free of charge) The EDMS MTF in Practice : 10.3.2004 (morning, free of charge) CLEAN-2002: Working in a Cleanroom : 10.3.2004 (afternoon, free of charge) The CERN EDMS for Engineers : 11.3.2004 (1 day, free of charge) LabVIEW hands-on (E):...

  5. Technical Training: Places available**

    CERN Document Server

    2004-01-01

    If you wish to participate in one of the following courses, please discuss with your supervisor and apply electronically directly from the course description pages that can be found on the Web at: http://www.cern.ch/Training/ or fill in an "application for training" form available from your Divisional Secretariat or from your DTO (Divisional Training Officer). Applications will be accepted in the order of their receipt. TECHNICAL TRAINING Monique Duval tel. 74924 technical.training@cern.ch ** The number of places available may vary. Please check our Web site to find out the current availability. Places are available in the following courses: The JAVA Programming Language Level 1 : 9 & 10.1.2004 (2 days) LabVIEW TestStand I (E) : 23 & 24.2.2004 (2 days) LabVIEW base 1 : 25 - 27.2.2004 (3 jours) CLEAN-2002 : Working in a Cleanroom : 10.3.2004 (afternoon - free of charge) C++ for Particle Physicists : 8 - 12.3.2004 ( 6 X 4-hour sessions) LabVIEW hands-on (E) 16.3...

  6. Technical Training: Places available

    CERN Multimedia

    2004-01-01

    If you wish to participate in one of the following courses, please discuss with your supervisor and apply electronically directly from the course description pages that can be found on the Web at: http://www.cern.ch/Training/ or fill in an "application for training" form available from your Divisional Secretariat or from your DTO (Divisional Training Officer). Applications will be accepted in the order of their receipt. TECHNICAL TRAINING Monique Duval tel. 74924 technical.training@cern.ch The number of places available may vary. Please check our Web site to find out the current availability. Places are available in the following courses: C++ for Particle Physicists : 8 - 12.3.2004 (6 X 4-hour sessions) Introduction to the CERN EDMS : 9.3.2004 (1 day, free of charge) The EDMS MTF in Practice : 10.3.2004 (morning, free of charge) CLEAN-2002 : Working in a Cleanroom : 10.3.2004 (afternoon, free of charge) The CERN EDMS for Engineers : 11.3.2004 (1 day, free of charge) LabVIEW...

  7. Academic Training: 2003 - 2004 Academic Training Programme

    CERN Multimedia

    2004-01-01

    ACADEMIC TRAINING Françoise Benz tel. 73127 academic.training@cern.ch 3rd Term - 5 April to 2nd July 2004 REGULAR LECTURE PROGRAMME 19, 20, 21, 22, 23 April Complex Systems, Chaos and Measurements by P. Collet / Ecole Polytechnique, Palaiseau, France 26, 27, 28, 29 April The Theory of Heavy Ion Collisions by U. Wiedemann / CERN-PH/TH 10, 11, 12, 13, 14 May Particle Identification at the LHC by D. Fournier / LAL, Orsay, France 1, 2, 3, 4 June Neural Systems, Genetic Algorithms by V. Robles Forcada and M. Perez Hernandez / Univ. Politecnica de Madrid E. 7, 8, 9, June Real Time Process Control by T. Riesco / CERN-TS 14, 15, 16, 17, 18 June The Cosmic Microwave Background by M. Zaldarriaga / Harvard University, USA 21, 22, 23, June Fixed Target Physics at CERN : Results and Prospects by J. Engelen / CERN-DG 28, 29, 30 June, 1, 2, July Search for Dark Matter by B. Sadoulet / Univ. of California, Berkeley, USA The lectures are open to all those interested, without application. The abstrac...

  8. Language Training : English Training

    CERN Document Server

    Françoise Benz

    2004-01-01

    General and Professional English Courses The next session will take place from 04 October 2004 to 11 February 2005 (3 weeks break at Christmas). These courses are open to all persons working on the CERN site, and to their spouses. For registration and further information on the courses, please consult our Web pages: http://cern.ch/Training or contact Mr. Liptow, tel. 72957. Oral Expression This course is intended for people with a good knowledge of English who want to practise and maintain their speaking skills while extending their vocabulary. There will be approximately 8 participants in a class. Speaking activities will include discussions, meeting simulations, role-plays etc. depending on the needs of the students. Duration: 20 hours (2 hours a week) Price: 440 CHF (for 8 students) For further information, please contact Mr. Liptow, tel. 72957. Date and timetable will be fixed when there are sufficient participants enrolled. FORMATION EN LANGUES LANGUAGE TRAINING Françoise Benz 73127 languag...

  9. Plant NAC-type transcription factor proteins contain a NARD domain for repression of transcriptional activation.

    Science.gov (United States)

    Hao, Yu-Jun; Song, Qing-Xin; Chen, Hao-Wei; Zou, Hong-Feng; Wei, Wei; Kang, Xu-Sheng; Ma, Biao; Zhang, Wan-Ke; Zhang, Jin-Song; Chen, Shou-Yi

    2010-10-01

    Plant-specific transcription factor NAC proteins play essential roles in many biological processes such as development, senescence, morphogenesis, and stress signal transduction pathways. In the NAC family, some members function as transcription activators while others act as repressors. In the present study we found that though the full-length GmNAC20 from soybean did not have transcriptional activation activity, the carboxy-terminal activation domain of GmNAC20 had high transcriptional activation activity in the yeast assay system. Deletion experiments revealed an active repression domain with 35 amino acids, named NARD (NAC Repression Domain), in the d subdomain of NAC DNA-binding domain. NARD can reduce the transcriptional activation ability of diverse transcription factors when fused to either the amino-terminal or the carboxy-terminal of the transcription factors. NARD-like sequences are also present in other NAC family members and they are functional repression domain when fused to VP16 in plant protoplast assay system. Mutation analysis of conserved amino acid residues in NARD showed that the hydrophobic LVFY motif may partially contribute to the repression function. It is hypothesized that the interactions between the repression domain NARD and the carboxy-terminal activation domain may finally determine the ability of NAC family proteins to regulate downstream gene expressions.

  10. Transcriptional regulation of topology modulators and transcription regulators of Mycobacterium tuberculosis.

    Science.gov (United States)

    Ghosh, Soumitra; Padmanabhan, Bhavna; Godbole, Adwait Anand; Tare, Priyanka; Ahmed, Wareed; Vasu, Kommireddy; China, Arnab; Kumar, Rupesh; Mitra, Anirban; Nagaraja, Valakunja

    2016-07-01

    Mycobacterium tuberculosis (Mtb) is a formidable pathogen which has the ability to survive the hostile environment of the host by evading the host defense system. The re-configuration of its transcriptional and metabolic process allows the pathogen to confront the adverse environment within the host macrophages. The factors that assist the transcription and modulate the DNA topology would have to play a key role in the regulation of global gene expression of the organism. How transcription of these essential housekeeping genes alters in response to growth conditions and environmental stress has not been addressed together in a set of experimental conditions in Mtb. Now, we have mapped the transcription start sites (TSS) and promoters of several genes that play a central role in the regulation of DNA topology and transcription in Mtb. Using in vivo reporter assays, we validated the activity of the identified promoter elements in different growth conditions. The variation in transcript abundance of these essential genes was also analyzed in growth phase-dependent manner. These data provide the first glimpse into the specific adaptive changes in the expression of genes involved in transcription and DNA topology modulation in Mtb.

  11. Training SVMs without offset

    Energy Technology Data Exchange (ETDEWEB)

    Steinwart, Ingo [Los Alamos National Laboratory; Hush, Don [Los Alamos National Laboratory; Scovel, Clint [Los Alamos National Laboratory

    2009-01-01

    We develop, analyze, and test a training algorithm for support vector machine cla.'>sifiers without offset. Key features of this algorithm are a new stopping criterion and a set of working set selection strategies that, although inexpensive, do not lead to substantially more iterations than the optimal working set selection strategy. For these working set strategies, we establish convergence rates that coincide with the best known rates for SYMs with offset. We further conduct various experiments that investigate both the run time behavior and the performed iterations of the new training algorithm. It turns out, that the new algorithm needs less iterations and run-time than standard training algorithms for SYMs with offset.

  12. INITIAL TRAINING OF RESEARCHERS

    Directory of Open Access Journals (Sweden)

    Karina Alejandra Cruz-Pallares

    2015-07-01

    Full Text Available The document presents results of a research that used as strategy a complementary training project with thirty-three students of a Bachelors Degree in Primary School 1997(DPS,1997 of an Education Faculty for the initial training of investigators, applied by four teachers members of the academic research group in Mexico; that develops through process of action research methodology. Highlighted in results is the strengthening of the competition of reading, understanding and writing scientific texts, which is analogous to the first feature of the graduate profile called intellectual skills. Among the conclusions it is emphasized that the initial training of teachers in a task that is quite interesting, challenging and complex, as is the educational complex phenomenon.

  13. Transportation safety training

    Energy Technology Data Exchange (ETDEWEB)

    Jones, E.

    1990-01-01

    Over the past 25 years extensive federal legislation involving the handling and transport of hazardous materials/waste has been passed that has resulted in numerous overlapping regulations administered and enforced by different federal agencies. The handling and transport of hazardous materials/waste involves a significant number of workers who are subject to a varying degree of risk should an accident occur during handling or transport. Effective transportation training can help workers address these risks and mitigate them, and at the same time enable ORNL to comply with the federal regulations concerning the transport of hazardous materials/waste. This presentation will outline how the Environmental and Health Protection Division's Technical Resources and Training Section at the Oak Ridge National Laboratory, working with transportation and waste disposal personnel, have developed and implemented a comprehensive transportation safety training program to meet the needs of our workers while satisfying appropriate federal regulations. 8 refs., 3 tabs.

  14. Simulators and endourological training.

    Science.gov (United States)

    Laguna, M Pilar; Hatzinger, Martin; Rassweiler, Jens

    2002-05-01

    Acquisition of skills laboratory training seems to be of importance in the training of surgeons, and is intended to cover the gap between theoretical learning and real practice. Economic and ethical reasons limit the use of animals during the learning process, while trends in medical change have severely restricted the available time to teach and to learn. With the incorporation of laparoscopy and the blossoming of minimally invasive techniques, mainly endoscopy, simulators have gained wide acceptance as an important tool in the surgeon's learning process. Two types of simulators are currently available: inanimates or mannequins and virtual reality simulators. A review of the recent literature shows that there is generally a significant improvement in dexterity after using simulators, whichever type is used. It is still unknown whether training simulation influences the patient's outcome positively.

  15. Alliance-focused training.

    Science.gov (United States)

    Eubanks-Carter, Catherine; Muran, J Christopher; Safran, Jeremy D

    2015-06-01

    Alliance-focused training (AFT) aims to increase therapists' ability to recognize, tolerate, and negotiate alliance ruptures by increasing the therapeutic skills of self-awareness, affect regulation, and interpersonal sensitivity. In AFT, therapists are encouraged to draw on these skills when metacommunicating about ruptures with patients. In this article, we present the 3 main supervisory tasks of AFT: videotape analysis of rupture moments, awareness-oriented role-plays, and mindfulness training. We describe the theoretical and empirical support for each supervisory task, provide examples based on actual supervision sessions, and present feedback about the usefulness of the techniques from trainees in our program. We also note some of the challenges involved in conducting AFT and the importance of maintaining a strong supervisory alliance when using this training approach.

  16. Simulation in resuscitation training.

    Science.gov (United States)

    Perkins, Gavin D

    2007-05-01

    The quality of education, CPR guidelines and the chain of survival all contribute to patient outcome following cardiac arrest. Increasing concerns about patient safety have focused attention on the methods used to train and prepare doctors for clinical practice. Reductions in clinical exposure at both undergraduate and postgraduate level have been implicated in junior doctors inability to recognise and manage critically ill patients. Simulation is used as a central training tool in contemporary advanced life support teaching. Simulation provides a learning opportunity for controlled clinical practice without putting patients or others at risk. This review examines the history and rationale for simulation training in resuscitation and provides some background to the learning theories that underpin it. The role of task trainers, high and low fidelity patient simulators and computer assisted simulation as teaching tools are discussed.

  17. E-groups training

    CERN Multimedia

    HR Department

    2012-01-01

    There will be an e-groups training course on 16 March 2012 which will cover the main e-groups functionalities i.e.: creating and managing e-groups, difference between static and dynamic e-groups, configuring posting restrictions and archives, examples of where e-groups can be used in daily work. Even if you have already worked with e-groups, this may be a good opportunity to learn about the best practices and security related recommendations when using e-groups. You can find more details as well as enrolment form for the training (it’s free) here. The number of places is limited, so enrolling early is recommended.   Technical Training Tel. 72844

  18. Technical Training: WBTechT offers online training

    CERN Multimedia

    Monique Duval

    2005-01-01

    CERN Technical Training 2005 The 2005 CERN Web-Based Technical Training portal is a computer-skills site offering multimedia learning. Visit http://www.course-source.net/sites/cern/ to self-register, consult the available programmes or request a course via EDH. A self-directed online course costs 50.- CHF for desktop applications and 90.- CHF for technical applications for three months' unlimited access. Visit the WBTechT portal or http://www.cern.ch/TechnicalTraining, and contact Technical.Training@cern.ch or your DTOs to find out more information. ENSEIGNEMENT TECHNIQUE TECHNICAL TRAINING Monique Duval 74924 technical.training@cern.ch

  19. Technical Training: WBTechT offers online training

    CERN Multimedia

    Monique Duval

    2005-01-01

    CERN Technical Training 2005 The 2005 CERN Web-Based Technical Training (WBTechT) portal is a computer-skills site offering multimedia learning. Visit http://www.course-source.net/sites/cern/ to self-register, consult the available programmes or request a course via EDH. A self-directed online course costs 50.- CHF for desktop applications and 90.- CHF for technical applications for three months' unlimited access. Visit the WBTechT portal or http://www.cern.ch/TechnicalTraining, and contact Technical.Training@cern.ch or your DTO to find out more information. ENSEIGNEMENT TECHNIQUE TECHNICAL TRAINING Monique Duval 74924 technical.training@cern.ch

  20. A compendium of nucleosome and transcript profiles reveals determinants of chromatin architecture and transcription.

    Directory of Open Access Journals (Sweden)

    Harm van Bakel

    2013-05-01

    Full Text Available Nucleosomes in all eukaryotes examined to date adopt a characteristic architecture within genes and play fundamental roles in regulating transcription, yet the identity and precise roles of many of the trans-acting factors responsible for the establishment and maintenance of this organization remain to be identified. We profiled a compendium of 50 yeast strains carrying conditional alleles or complete deletions of genes involved in transcriptional regulation, histone biology, and chromatin remodeling, as well as compounds that target transcription and histone deacetylases, to assess their respective roles in nucleosome positioning and transcription. We find that nucleosome patterning in genes is affected by many factors, including the CAF-1 complex, Spt10, and Spt21, in addition to previously reported remodeler ATPases and histone chaperones. Disruption of these factors or reductions in histone levels led genic nucleosomes to assume positions more consistent with their intrinsic sequence preferences, with pronounced and specific shifts of the +1 nucleosome relative to the transcription start site. These shifts of +1 nucleosomes appear to have functional consequences, as several affected genes in Ino80 mutants exhibited altered expression responses. Our parallel expression profiling compendium revealed extensive transcription changes in intergenic and antisense regions, most of which occur in regions with altered nucleosome occupancy and positioning. We show that the nucleosome-excluding transcription factors Reb1, Abf1, Tbf1, and Rsc3 suppress cryptic transcripts at their target promoters, while a combined analysis of nucleosome and expression profiles identified 36 novel transcripts that are normally repressed by Tup1/Cyc8. Our data confirm and extend the roles of chromatin remodelers and chaperones as major determinants of genic nucleosome positioning, and these data provide a valuable resource for future studies.

  1. A compendium of nucleosome and transcript profiles reveals determinants of chromatin architecture and transcription.

    Science.gov (United States)

    van Bakel, Harm; Tsui, Kyle; Gebbia, Marinella; Mnaimneh, Sanie; Hughes, Timothy R; Nislow, Corey

    2013-05-01

    Nucleosomes in all eukaryotes examined to date adopt a characteristic architecture within genes and play fundamental roles in regulating transcription, yet the identity and precise roles of many of the trans-acting factors responsible for the establishment and maintenance of this organization remain to be identified. We profiled a compendium of 50 yeast strains carrying conditional alleles or complete deletions of genes involved in transcriptional regulation, histone biology, and chromatin remodeling, as well as compounds that target transcription and histone deacetylases, to assess their respective roles in nucleosome positioning and transcription. We find that nucleosome patterning in genes is affected by many factors, including the CAF-1 complex, Spt10, and Spt21, in addition to previously reported remodeler ATPases and histone chaperones. Disruption of these factors or reductions in histone levels led genic nucleosomes to assume positions more consistent with their intrinsic sequence preferences, with pronounced and specific shifts of the +1 nucleosome relative to the transcription start site. These shifts of +1 nucleosomes appear to have functional consequences, as several affected genes in Ino80 mutants exhibited altered expression responses. Our parallel expression profiling compendium revealed extensive transcription changes in intergenic and antisense regions, most of which occur in regions with altered nucleosome occupancy and positioning. We show that the nucleosome-excluding transcription factors Reb1, Abf1, Tbf1, and Rsc3 suppress cryptic transcripts at their target promoters, while a combined analysis of nucleosome and expression profiles identified 36 novel transcripts that are normally repressed by Tup1/Cyc8. Our data confirm and extend the roles of chromatin remodelers and chaperones as major determinants of genic nucleosome positioning, and these data provide a valuable resource for future studies.

  2. Cross-Family Transcription Factor Interactions

    NARCIS (Netherlands)

    Bemer, Marian; Dijk, van Aalt-Jan; Immink, Richard G.H.; Angenent, Gerco C.

    2017-01-01

    Specific and dynamic gene expression strongly depends on transcription factor (TF) activity and most plant TFs function in a combinatorial fashion. They can bind to DNA and control the expression of the corresponding gene in an additive fashion or cooperate by physical interactions, forming larger p

  3. Transcriptional enhancer from milk protein genes

    Energy Technology Data Exchange (ETDEWEB)

    Casperson, Gerald F. (Ballwin, MO); Schmidhauser, Christian T. (Berkeley, CA); Bissell, Mina J. (Berkeley, CA)

    1999-01-01

    The invention relates to novel enhancer nucleotide sequences which stimulate transcription of heterologous DNA in cells in culture. The enhancers are derived from major milk protein genes by the process of deletion mapping and functional analysis. The invention also relates to expression vectors containing the novel enhancers.

  4. Transcriptional enhancer from milk protein genes

    Energy Technology Data Exchange (ETDEWEB)

    Casperson, G.F.; Schmidhauser, C.T.; Bissell, M.J.

    1999-12-21

    The invention relates to novel enhancer nucleotide sequences which stimulate transcription of heterologous DNA in cells in culture. The enhancers are derived from major milk protein genes by the process of deletion mapping and functional analysis. The invention also relates to expression vectors containing the novel enhancers.

  5. 20 CFR 901.47 - Transcript.

    Science.gov (United States)

    2010-04-01

    ... 20 Employees' Benefits 3 2010-04-01 2010-04-01 false Transcript. 901.47 Section 901.47 Employees' Benefits JOINT BOARD FOR THE ENROLLMENT OF ACTUARIES REGULATIONS GOVERNING THE PERFORMANCE OF ACTUARIAL SERVICES UNDER THE EMPLOYEE RETIREMENT INCOME SECURITY ACT OF 1974 Suspension or Termination of...

  6. Polyphenol Compound as a Transcription Factor Inhibitor

    Directory of Open Access Journals (Sweden)

    Seyeon Park

    2015-10-01

    Full Text Available A target-based approach has been used to develop novel drugs in many therapeutic fields. In the final stage of intracellular signaling, transcription factor–DNA interactions are central to most biological processes and therefore represent a large and important class of targets for human therapeutics. Thus, we focused on the idea that the disruption of protein dimers and cognate DNA complexes could impair the transcriptional activation and cell transformation regulated by these proteins. Historically, natural products have been regarded as providing the primary leading compounds capable of modulating protein–protein or protein-DNA interactions. Although their mechanism of action is not fully defined, polyphenols including flavonoids were found to act mostly as site-directed small molecule inhibitors on signaling. There are many reports in the literature of screening initiatives suggesting improved drugs that can modulate the transcription factor interactions responsible for disease. In this review, we focus on polyphenol compound inhibitors against dimeric forms of transcription factor components of intracellular signaling pathways (for instance, c-jun/c-fos (Activator Protein-1; AP-1, c-myc/max, Nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB and β-catenin/T cell factor (Tcf.

  7. Transcriptional networks in epithelial-mesenchymal transition.

    Directory of Open Access Journals (Sweden)

    Christo Venkov

    Full Text Available Epithelial-mesenchymal transition (EMT changes polarized epithelial cells into migratory phenotypes associated with loss of cell-cell adhesion molecules and cytoskeletal rearrangements. This form of plasticity is seen in mesodermal development, fibroblast formation, and cancer metastasis.Here we identify prominent transcriptional networks active during three time points of this transitional process, as epithelial cells become fibroblasts. DNA microarray in cultured epithelia undergoing EMT, validated in vivo, were used to detect various patterns of gene expression. In particular, the promoter sequences of differentially expressed genes and their transcription factors were analyzed to identify potential binding sites and partners. The four most frequent cis-regulatory elements (CREs in up-regulated genes were SRY, FTS-1, Evi-1, and GC-Box, and RNA inhibition of the four transcription factors, Atf2, Klf10, Sox11, and SP1, most frequently binding these CREs, establish their importance in the initiation and propagation of EMT. Oligonucleotides that block the most frequent CREs restrain EMT at early and intermediate stages through apoptosis of the cells.Our results identify new transcriptional interactions with high frequency CREs that modulate the stability of cellular plasticity, and may serve as targets for modulating these transitional states in fibroblasts.

  8. Transcriptional control of hepatocanalicular transporter gene expression

    NARCIS (Netherlands)

    Muller, M

    2000-01-01

    Transport processes for larger organic solutes at the canalicular membrane are mainly driven by members of the superfamily of ATP-binding cassette (ABC) transporters. The funct ions of these transporters range from bile component secretion to xenobiotica and phase II-conjugate export. The transcript

  9. The Lrp family of transcriptional regulators

    NARCIS (Netherlands)

    Brinkman, A.B.; Ettema, T.J.G.; Vos, de W.M.; Oost, van der J.

    2003-01-01

    Genome analysis has revealed that members of the Lrp family of transcriptional regulators are widely distributed among prokaryotes, both bacteria and archaea. The archetype Leucine-responsive Regulatory Protein from Escherichia coli is a global regulator involved in modulating a variety of metabolic

  10. Mitochondrial transcription: How does it end

    Energy Technology Data Exchange (ETDEWEB)

    J Byrnes; M Garcia-Diaz

    2011-12-31

    The structure of the mitochondrial transcription termination factor (MTERF1) provides novel insight into the mechanism of binding, recognition of the termination sequence and the conformational changes involved in mediating termination. Besides its functional implications, this structure provides a framework to understand the consequences of numerous diseases associated with mitochondrial DNA mutations.

  11. TRANSFAC: transcriptional regulation, from patterns to profiles.

    Science.gov (United States)

    Matys, V; Fricke, E; Geffers, R; Gössling, E; Haubrock, M; Hehl, R; Hornischer, K; Karas, D; Kel, A E; Kel-Margoulis, O V; Kloos, D-U; Land, S; Lewicki-Potapov, B; Michael, H; Münch, R; Reuter, I; Rotert, S; Saxel, H; Scheer, M; Thiele, S; Wingender, E

    2003-01-01

    The TRANSFAC database on eukaryotic transcriptional regulation, comprising data on transcription factors, their target genes and regulatory binding sites, has been extended and further developed, both in number of entries and in the scope and structure of the collected data. Structured fields for expression patterns have been introduced for transcription factors from human and mouse, using the CYTOMER database on anatomical structures and developmental stages. The functionality of Match, a tool for matrix-based search of transcription factor binding sites, has been enhanced. For instance, the program now comes along with a number of tissue-(or state-)specific profiles and new profiles can be created and modified with Match Profiler. The GENE table was extended and gained in importance, containing amongst others links to LocusLink, RefSeq and OMIM now. Further, (direct) links between factor and target gene on one hand and between gene and encoded factor on the other hand were introduced. The TRANSFAC public release is available at http://www.gene-regulation.com. For yeast an additional release including the latest data was made available separately as TRANSFAC Saccharomyces Module (TSM) at http://transfac.gbf.de. For CYTOMER free download versions are available at http://www.biobase.de:8080/index.html.

  12. Virtual Reference Transcript Analysis: A Few Models.

    Science.gov (United States)

    Smyth, Joanne

    2003-01-01

    Describes the introduction of virtual, or digital, reference service at the University of New Brunswick libraries. Highlights include analyzing transcripts from LIVE (Library Information in a Virtual Environment); reference question types; ACRL (Association of College and Research Libraries) information literacy competency standards; and the Big 6…

  13. 40 CFR 164.82 - Transcripts.

    Science.gov (United States)

    2010-07-01

    ... 40 Protection of Environment 23 2010-07-01 2010-07-01 false Transcripts. 164.82 Section 164.82 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) PESTICIDE PROGRAMS RULES OF PRACTICE GOVERNING HEARINGS, UNDER THE FEDERAL INSECTICIDE, FUNGICIDE, AND RODENTICIDE ACT, ARISING FROM REFUSALS...

  14. Harmonics of circadian gene transcription in mammals.

    Directory of Open Access Journals (Sweden)

    Michael E Hughes

    2009-04-01

    Full Text Available The circadian clock is a molecular and cellular oscillator found in most mammalian tissues that regulates rhythmic physiology and behavior. Numerous investigations have addressed the contribution of circadian rhythmicity to cellular, organ, and organismal physiology. We recently developed a method to look at transcriptional oscillations with unprecedented precision and accuracy using high-density time sampling. Here, we report a comparison of oscillating transcription from mouse liver, NIH3T3, and U2OS cells. Several surprising observations resulted from this study, including a 100-fold difference in the number of cycling transcripts in autonomous cellular models of the oscillator versus tissues harvested from intact mice. Strikingly, we found two clusters of genes that cycle at the second and third harmonic of circadian rhythmicity in liver, but not cultured cells. Validation experiments show that 12-hour oscillatory transcripts occur in several other peripheral tissues as well including heart, kidney, and lungs. These harmonics are lost ex vivo, as well as under restricted feeding conditions. Taken in sum, these studies illustrate the importance of time sampling with respect to multiple testing, suggest caution in use of autonomous cellular models to study clock output, and demonstrate the existence of harmonics of circadian gene expression in the mouse.

  15. ETS transcription factors in embryonic vascular development.

    Science.gov (United States)

    Craig, Michael P; Sumanas, Saulius

    2016-07-01

    At least thirteen ETS-domain transcription factors are expressed during embryonic hematopoietic or vascular development and potentially function in the formation and maintenance of the embryonic vasculature or blood lineages. This review summarizes our current understanding of the specific roles played by ETS factors in vasculogenesis and angiogenesis and the implications of functional redundancies between them.

  16. Insights into centromeric transcription in mitosis.

    Science.gov (United States)

    Liu, Hong

    2016-01-01

    The major role of RNA polymerase II (RNAP II) is to generate mRNAs. I recently uncovered a novel function of RNAP II in chromosome segregation in mitosis, installing the cohesin protector, Shugoshin, at centromeres. Here I will discuss the current understanding of RNAP II-dependent centromeric transcription in mitosis.

  17. Transcriptional inhibition by the retinoblastoma protein

    DEFF Research Database (Denmark)

    Fattaey, A; Helin, K; Harlow, E

    1993-01-01

    The retinoblastoma protein, pRB, appears to play a key role in coordinating the regulation of cell cycle position and transcriptional events. pRB undergoes specific cell-cycle-dependent phosphorylation, being underphosphorylated in G1 and heavily phosphorylated in S, G2, and M. The underphosphory......The retinoblastoma protein, pRB, appears to play a key role in coordinating the regulation of cell cycle position and transcriptional events. pRB undergoes specific cell-cycle-dependent phosphorylation, being underphosphorylated in G1 and heavily phosphorylated in S, G2, and M....... The underphosphorylated form is able to interact with the E2F transcription factor. Recently, we have cloned a cDNA for E2F-1. By using this clone and a series of non-pRB binding mutants, we have been able to show that the binding of pRB to E2F-1 causes inhibition of E2F-mediated transactivation. pRB's inhibition of E2F......-mediated transcription would be lost by mutation in the retinoblastoma gene in human tumours, by pRB's interaction with DNA tumour virus oncoproteins, or by phosphorylation during the cell cycle....

  18. Interaction of Restin with transcription factors

    Institute of Scientific and Technical Information of China (English)

    WU; Yousheng; LU; Fan; QI; Yinxin; WANG; Ruihua; ZHANG; Jia

    2005-01-01

    Restin, a member of melanoma-associated antigen superfamily gene, was first cloned from differentiated leukemia cell induced by all trans-retinoic acid, and was able to inhibit cell proliferation, but the molecular mechanism was not clear. Since Restin was localized in cell nucleus, and its homolog member, Necdin (neuronal growth suppressor factor), could interact with transcription factors p53 and E2F1, we proposed that Restin might also function as Necdin through interacting with some transcription factors. In this study, transcription factors p53, AP1,ATFs and E2Fs were cloned and used in the mammalian two-hybrid system to identify their interaction with Restin. The results showed that only ATF3 had a strong interaction with Restin. It is interesting to know that ATF3 was an important transcription factor for G1 cell cycle initiation in physiological stress response. It was possible that the inhibition of cell proliferation by Restin might be related with the inhibition of ATF3 activity.

  19. Transcriptional Responses to the Auxin Hormone

    NARCIS (Netherlands)

    Weijers, Dolf; Wagner, Doris

    2016-01-01

    Auxin is arguably the most important signaling molecule in plants, and the last few decades have seen remarkable breakthroughs in understanding its production, transport, and perception. Recent investigations have focused on transcriptional responses to auxin, providing novel insight into the fun

  20. Regulation of the Ets transcription factor Tel

    NARCIS (Netherlands)

    Roukens, Mark Guido

    2010-01-01

    In this thesis we report novel studies on the molecular regulation of the transcriptional repressor Tel (Translocation Ets Leukemia). The work in this thesis is presented as follows: Chapter 1 is an introduction which summarizes the literature about Tel and its Drosophila orthologue Yan as it was k

  1. NAC transcription factors: structurally distinct, functionally diverse

    DEFF Research Database (Denmark)

    Olsen, Addie Nina; Ernst, Heidi A; Leggio, Leila Lo;

    2005-01-01

    NAC proteins constitute one of the largest families of plant-specific transcription factors, and the family is present in a wide range of land plants. Here, we summarize the biological and molecular functions of the NAC family, paying particular attention to the intricate regulation of NAC protei...

  2. TCP transcription factors: architectures of plant form.

    Science.gov (United States)

    Manassero, Nora G Uberti; Viola, Ivana L; Welchen, Elina; Gonzalez, Daniel H

    2013-04-01

    After its initial definition in 1999, the TCP family of transcription factors has become the focus of a multiplicity of studies related with plant development at the cellular, organ, and tissue levels. Evidence has accumulated indicating that TCP transcription factors are the main regulators of plant form and architecture and constitute a tool through which evolution shapes plant diversity. The TCP transcription factors act in a multiplicity of pathways related with cell proliferation and hormone responses. In recent years, the molecular pathways of TCP protein action and biochemical studies on their mode of interaction with DNA have begun to shed light on their mechanism of action. However, the available information is fragmented and a unifying view of TCP protein action is lacking, as well as detailed structural studies of the TCP-DNA complex. Also important, the possible role of TCP proteins as integrators of plant developmental responses to the environment has deserved little attention. In this review, we summarize the current knowledge about the structure and functions of TCP transcription factors and analyze future perspectives for the study of the role of these proteins and their use to modify plant development.

  3. Mitochondrial transcription: how does it end?

    Science.gov (United States)

    Byrnes, James; Garcia-Diaz, Miguel

    2011-01-01

    The structure of the mitochondrial transcription termination factor (MTERF1) provides novel insight into the mechanism of binding, recognition of the termination sequence and the conformational changes involved in mediating termination. Besides its functional implications, this structure provides a framework to understand the consequences of numerous diseases associated with mitochondrial DNA mutations.

  4. Genetic and epigenetic control of RKIP transcription.

    Science.gov (United States)

    Datar, Ila; Tegegne, Hanna; Qin, Kevin; Al-Mulla, Fahd; Bitar, Milad S; Trumbly, Robert J; Yeung, Kam C

    2014-01-01

    Raf kinase inhibitory protein (RKIP) is known to modulate key signaling cascades and regulate normal physiological processes such as cellular proliferation, differentiation, and apoptosis. The expression of RKIP is found to be downregulated in several cancer metastases and the repressed RKIP expression can be reactivated on treatment with chemotherapeutic agents. RKIP is a proven tumor metastasis suppressor gene and investigating the mechanisms of transcriptional regulation of RKIP is therefore of immense clinical importance. In this review, we discuss the basal expression of RKIP in various tissues and the genetic aspects of the RKIP chromosomal locus including the structure of the RKIP promoter as well as gene regulatory elements such as enhancers. We also review the genetic and epigenetic modulation of RKIP transcription through EZH2, a component of the polycomb repressive complex 2 (PRC2) and sequence specific transcription factors (TFs) BACH1 and Snail. Emerging experimental evidence supports a unifying model in which both these TFs repress RKIP transcription in cancers by recruiting the EZH2 containing repressive complex to the proximal RKIP promoter. Finally, we review the known mechanisms employed by different types of chemotherapeutic agents to activate RKIP expression in cancer cells.

  5. Systematic clustering of transcription start site landscapes

    DEFF Research Database (Denmark)

    Zhao, Xiaobei; Valen, Eivind; Parker, Brian J;

    2011-01-01

    Genome-wide, high-throughput methods for transcription start site (TSS) detection have shown that most promoters have an array of neighboring TSSs where some are used more than others, forming a distribution of initiation propensities. TSS distributions (TSSDs) vary widely between promoters...

  6. Nuclear education and training

    CERN Document Server

    2012-01-01

    The OECD Nuclear Energy Agency (NEA) first published in 2000 Nuclear Education and Training: Cause for Concern? , which highlighted significant issues in the availability of human resources for the nuclear industry. Ten years on, Nuclear Education and Training: From Concern to Capability considers what has changed in that time and finds that, while some countries have taken positive actions, in a number of others human resources could soon be facing serious challenges in coping with existing and potential new nuclear facilities. This is exacerbated by the increasing rate of retirement as the w

  7. [Hand surgery training].

    Science.gov (United States)

    Moutet, F; Haloua, J P

    2003-10-01

    Training of the hand surgeon HAND SURGEON A CONCEPT: The hand surgeon is supposed to be in charge of all the hand lesions regarding, skeleton, muscles, tendons, nerves and vessels. He has to be able to insure reparation and coverage of all of them. So he is involved in all the structures, which insure integrity and function of the hand. PURPOSE AND WAYS OF TRAINING: To obtain the asked ability, the hand surgeon training has to be global and sustained by two underlying surgical specialities: orthopedic surgery and plastic and reconstructive surgery. From 2000 after many years of dealings, a Right to the Title in Hand Surgery was born. This Right to the Title wants to be the formal recognition of the specific training of the hand surgeon. For the well-recognized ancient hand surgeons they need to be confirmed by one's peers. Now a day the hand surgeon has to satisfy to this specific training: Passed the complete training and exam of the Orthopedic or Plastic surgery board. Spent at least 6 months as resident in the other underlying specialty. Passed a microsurgery examination. Passed one of the four national Hand Surgery diplomas (DIU/Inter-Universitary Diploma). The examinations have been harmonized. A common formation is delivered regarding hand surgery, the way of examination is the same and the formation is 2 years long. The final exam is presented in front of board of examiners where a teacher of one of the other three national diplomas is present. Spent at least 2 years in a formative hand surgery unit, listed by the French College of Hand Surgeons, as senior surgeon. Those requirements are heavy to assume and need a heavy personal involvement. That seems to be necessary to have an ability level as high as possible. Emergency surgery practice is absolutely necessary in this training. All the 17 university formative hand surgery units listed by the French College of Hand Surgeons are members of the FESUM (European Federation of the Emergency Hand Units

  8. National Biocontainment Training Center

    Science.gov (United States)

    2010-06-01

    experimentally infected animals, including non- human primates, under containment conditions. As our animal handling in containment training module...Theoretical non 16 0 0 0 16 human primate BSL3 mentorship 26 10 0 17 53 Specialized 0 18 0 17 35 training (Assay Development division) BSL3...39% 4% 11% Su ent:fk f acdity Jnd M<.)nlen<.tn ct~ ··· Onwr> (IT, i\\RC, Non ;ocntdK) tr High >chool Underr . rdd Stude nt Graduate St u(~cnt s

  9. Turning training into play

    DEFF Research Database (Denmark)

    Aarhus, Rikke; Grönvall, Erik; Larsen, Simon Bo;

    2011-01-01

    Embodied gaming has been adopted and gained credibility in the field of physical rehabilitation. In this paper, we report on findings from a six-month-long study of three groups of senior citizens, and their use of Nintendo Wii Fit in a supervised physical training context. We argue that the study...... participants generally found physical training both fun and socially engaging, and experienced improved fitness. We also argue that embodied gaming motivates seniors to do more than they think themselves capable of, and allows seniors with different mental and physical capabilities to play together. However...

  10. In Vitro Transcription Assays and Their Application in Drug Discovery.

    Science.gov (United States)

    Yang, Xiao; Ma, Cong

    2016-09-20

    In vitro transcription assays have been developed and widely used for many years to study the molecular mechanisms involved in transcription. This process requires multi-subunit DNA-dependent RNA polymerase (RNAP) and a series of transcription factors that act to modulate the activity of RNAP during gene expression. Sequencing gel electrophoresis of radiolabeled transcripts is used to provide detailed mechanistic information on how transcription proceeds and what parameters can affect it. In this paper we describe the protocol to study how the essential elongation factor NusA regulates transcriptional pausing, as well as a method to identify an antibacterial agent targeting transcription initiation through inhibition of RNAP holoenzyme formation. These methods can be used a as platform for the development of additional approaches to explore the mechanism of action of the transcription factors which still remain unclear, as well as new antibacterial agents targeting transcription which is an underutilized drug target in antibiotic research and development.

  11. Statistical Mechanics of Transcription-Factor Binding Site Discovery Using Hidden Markov Models.

    Science.gov (United States)

    Mehta, Pankaj; Schwab, David J; Sengupta, Anirvan M

    2011-04-01

    Hidden Markov Models (HMMs) are a commonly used tool for inference of transcription factor (TF) binding sites from DNA sequence data. We exploit the mathematical equivalence between HMMs for TF binding and the "inverse" statistical mechanics of hard rods in a one-dimensional disordered potential to investigate learning in HMMs. We derive analytic expressions for the Fisher information, a commonly employed measure of confidence in learned parameters, in the biologically relevant limit where the density of binding sites is low. We then use techniques from statistical mechanics to derive a scaling principle relating the specificity (binding energy) of a TF to the minimum amount of training data necessary to learn it.

  12. Adding flexibility to physician training.

    Science.gov (United States)

    Mahady, Suzanne E

    2011-05-02

    Demographic changes among junior doctors are driving demand for increased flexibility in advanced physician training, but flexible training posts are lacking. Suitable flexible training models include flexible full-time, job-share and part-time positions. Major barriers to establishing flexible training positions include difficulty in finding job-share partners, lack of funding for creating supernumerary positions, and concern over equivalence of educational quality compared with full-time training. Pilot flexible training positions should be introduced across the medical specialties and educational outcomes examined prospectively.

  13. Strength training and aerobic exercise training for muscle disease (Review)

    NARCIS (Netherlands)

    Voet, N.B.M.; Kooi, E.L. van der; Riphagen, I.I.; Lindeman, E.; Engelen, B.G.M. van; Geurts, A.C.H.

    2010-01-01

    BACKGROUND: Strength training or aerobic exercise programmes might optimise muscle and cardiorespiratory function and prevent additional disuse atrophy and deconditioning in people with a muscle disease. OBJECTIVES: To examine the safety and efficacy of strength training and aerobic exercise trainin

  14. 30 CFR 75.834 - Training.

    Science.gov (United States)

    2010-07-01

    ... 30 Mineral Resources 1 2010-07-01 2010-07-01 false Training. 75.834 Section 75.834 Mineral....834 Training. In addition to existing part 48 task training, hazard training, training for qualified persons under existing § 75.153, and annual refresher training, the following specialized training...

  15. Constructing Virtual Training Demonstrations

    Science.gov (United States)

    2008-12-01

    Taylor, Russ-Eft, & Chan, 2005). BMT is based on Bandura’s social learning theory (Hogan, Hakel, & Decker, 1986). Utilizing the model provided by... social learning theory , BMT includes processes such as modeling, a retention process, behavioral rehearsal, feedback, and methods of training transfer

  16. Training for Leadership.

    Science.gov (United States)

    Adair, John

    An indepth study of how to select and train for effective leadership is based on the belief that though innate character traits play a role of leadership, leadership potential can be developed. The author's theory known as "functional leadership," stresses that leadership is an interaction among leader, group members, and situation. The good…

  17. Aircrew Combat Preparation Training

    Science.gov (United States)

    1989-07-21

    oo0.ovs8 la.’REPORT SECURITY CLASSIFICATION lb RESTRICTIVE MARKINGS Unclassified 2a. SECURITY CLASSIFICATION AUTHORITY 3 DISTRIBUTION/ AVAILABILTY OF...DESCRIPTION: Theobjectiveof this studyis to formulate guidelines fordesigning training courseware that allow full usage of the capabilities of a given

  18. Technical Training: Places available

    CERN Multimedia

    Monique Duval

    2005-01-01

    The number of places available may vary. Please check our Web site to find out the current availability. Places are available on the following courses: LabVIEW Migration 6 to 7: 14.6.2005 (1 day) IT3T/1 - Read your mail and more with Outlook 2003 : 14.6.2005 (IT Technical Training Tutorial, free of charge) IT3T/2 - Creating, managing and using distribution lists with Simba2 : 16.6.2005 (IT Technical Training Tutorial, free of charge) FrontPage 2003 - niveau 2 : 16 & 17.6.2005 (2 jours) size="2">(1journée) Hands-on Introduction to Python Programming: 28 - 30.6.2005 (3 days) Introduction to ANSYS: 21 - 24.6.2005 (4 days) IT3T/3 - Working remotely with Windows XP: 28.6.2005 (IT Technical Training Tutorial, free of charge) IT3T/4 - Editing Websites with Frontpage 2003: 30.6.2005 (IT Technical Training Tutorial, free of charge) LabVIEW base 1 : 4 - 6.7.2005 (3 jours) LabVIEW Basics 2: AutoCorrect, Find/Replace) : 4.7.2005 (afternoon) WORD 2003 (Shor...

  19. Technical Training: Places available

    CERN Multimedia

    Monique Duval

    2005-01-01

    The number of places available may vary. Please check our Web site to find out the current availability. Places are available on the following courses: LabVIEW Real-Time (F) : 7 - 9.6.2005 (3 jours) LabVIEW Migration 6 to 7: 14.6.2005 (1 day) IT3T/1 - Read your mail and more with Outlook 2003 : 14.6.2005 (IT Technical Training Tutorial, free of charge) IT3T/2 - Creating, managing and using distribution lists with Simba2 : 16.6.2005 (IT Technical Training Tutorial, free of charge) FrontPage 2003 - niveau 2 : 16 & 17.6.2005 (2 jours) Utilisation de fichiers PDF avec ACROBAT 6.0 : 20.6.2005 (1journée) Introduction to ANSYS: 21 - 24.6.2005 (4 days) IT3T/3 - Working remotely with Windows XP : 28.6.2005 (IT Technical Training Tutorial, free of charge) IT3T/4 - Editing Websites with Frontpage 2003 : 30.6.2005 (IT Technical Training Tutorial, free of charge) WORD 2003 (Short Course I) - HowTo... Work with repetitive tasks /AutoText, AutoFormat, AutoC...

  20. Gamification for astronaut training

    NARCIS (Netherlands)

    Cornelissen, F.; Neerincx, M.A.; Smets, N.J.J.M.; Breebaart, L.; Dujardin, P.; Wolff, M.

    2012-01-01

    This paper reports on the use and the evaluation of applying gaming aspects as a means to promote self-study and increase motivation to train for executing operations on human space flight missions that have a duration that exceeds the typical duration of low earth orbit missions. The gaming aspects

  1. Technical Training: Places available

    CERN Multimedia

    Monique Duval

    2004-01-01

    If you wish to participate in one of the following courses, please discuss with your supervisor and apply electronically directly from the course description pages that can be found on the Web at: http://www.cern.ch/Training/ or fill in an 'application for training' form available from your Divisional Secretariat or from your DTO (Divisional Training Officer). Applications will be accepted in the order of their receipt. The number of places available may vary. Please check our Web site to find out the current availability. Places are available in the following courses: AutoCAD 2002 - niveau 1 : 13, 14, 23, 24.09.2004 (4 jours) Introduction to the CERN EDMS : 22.6.2004 (1 day) The CERN EDMS for local administrators : 23 & 24.6.2004 (2 days) MAGNE-04 : Magnétisme pour l'électrotechnique : 6 - 8.7.2004 (3 jours) Introduction au VHDL et utilisation du simulateur NCVHDL de CADENCE : 7 & 8.9.2004 (2 jours) ENSEIGNEMENT TECHNIQUE TECHNICAL TRAINING...

  2. Teacher-Training Projects

    Science.gov (United States)

    Greer, Leslie

    1977-01-01

    The Sociedade Brasileira de Cultura Inglesa of Sao Paolo, Brazil, is an English teaching center which also runs an introductory course to train teachers of English. This article describes some of the projects completed by prospective teachers; they include language games, pictures, cartoons, role-playing and writing creative dialogue. (CHK)

  3. Language Training: French

    CERN Multimedia

    Françoise Benz

    2004-01-01

    If you wish to participate in one of the following courses, please discuss with your supervisor and apply electronically directly from the course description pages that can be found on the Web at: http://www.cern.ch/Training/ or fill in an 'application for training' form available from your Divisional Secretariat or from your DTO (Divisional Training Officer). Applications will be accepted in the order of their receipt.General and Professional French Courses The next session will take place from 26 April to 02 July 2004. These courses are open to all persons working on the Cern site, and to their spouses. For registration and further information on the courses, please consult our Web pages: http://cern.ch/Training or contact Mrs. Benz: Tel. 73127. Writing Professional Documents in French The next session will take place from 26 April to 02 July 2004. This course is designed for people with a good level of spoken French. Duration: 30 hours Price: 660 CHF (for 8 students) For further information and registra...

  4. Dental Training Films.

    Science.gov (United States)

    Veterans Administration Medical Center, Washington, DC.

    This dental training films catalog is organized into two sections. Section I is a category listing of the films by number and title, indexed according to generalized headings; categories are as follow: anatomy, articulator systems, complete dentures, dental assisting, dental laboratory technology, dental materials, dental office emergencies,…

  5. Slow Train Coming

    Science.gov (United States)

    McNair, Stephen

    2011-01-01

    The battle for an adequate careers guidance service for adults has been a long one. A succession of governments have been formally committed to lifelong learning, to broadening choice and diversity in education and training, and to asking individuals to take more control of (and pay more for) their education, but only in the last few years have…

  6. Technician Training Experience

    CERN Multimedia

    2015-01-01

    There is a real shortage of skilled engineers & technicians in Europe and yet at the same time high unemployment levels. For years CERN has offered a great training ground for engineers & physicists and we are now aiming to do the same for technicians. Th

  7. Hard Training Bears Fruit

    Institute of Scientific and Technical Information of China (English)

    1996-01-01

    Perfect skills take time to acquire. An outstanding performance, while it may be short, is the harvest of many years’ training. Hearty rounds of applause Reward the artists’ diligence. The flowers of the arts are fertilized with sweat; Consummate skills bridge all ages, And new always springs from old

  8. Vocational Training in Ireland.

    Science.gov (United States)

    Dooney, Roy; Dunne, Paul

    This monograph, one of a series of studies of vocational education in the countries of the European Communities, describes the vocational training system in Ireland. The study was compiled from existing statistics and descriptions, and most figures cited refer to 1984. The report is organized in eight chapters. Chapter 1 covers population,…

  9. Basic Science Training Program.

    Science.gov (United States)

    Brummel, Clete

    These six learning modules were developed for Lake Michigan College's Basic Science Training Program, a workshop to develop good study skills while reviewing basic science. The first module, which was designed to provide students with the necessary skills to study efficiently, covers the following topics: time management; an overview of a study…

  10. Training International Journalists.

    Science.gov (United States)

    Bartram, John

    2000-01-01

    Examines eight key issues in training journalists. Argues: journalism educators should still be teaching the basic journalism skills that are of practical use in the marketplace; they should not show an exaggerated interest in the Ph.D. research that attracts so much coveted university funding; and they must try to put the interests and needs of…

  11. The Effect of Communication Strategy Training on the Development of EFL Learners' Strategic Competence and Oral Communicative Ability.

    Science.gov (United States)

    Rabab'ah, Ghaleb

    2016-06-01

    This study examines the effect of communication strategy instruction on EFL students' oral communicative ability and their strategic competence. In a 14-week English as a Foreign Language (EFL) course (English Use II) based on Communicative Language Teaching approach, 80 learners were divided into two groups. The strategy training group ([Formula: see text]) received CS training based on a training program designed for the purpose of the present research, whereas the control group ([Formula: see text]) received only the normal communicative course using Click On 3, with no explicit focus on CSs. The communication strategies targeted in the training program included circumlocution (paraphrase), appeal for help, asking for repetition, clarification request, confirmation request, self-repair, and guessing. Pre- and post-test procedures were used to find out the effect of strategy training on language proficiency and CS use. The effect of the training was assessed by three types of data collection: the participants' pre- and post-IELTS speaking test scores, transcription data from the speaking IELTS test, and 'Click On' Exit Test scores. The findings revealed that participants in the strategy training group significantly outperformed the control group in their IELTS speaking test scores. The results of the post-test transcription data also confirmed that the participants in the strategy training group used more CSs, which could be attributed to the CS training program. The findings of the present research have implications for language teachers, and syllabus designers.

  12. Early Exercise Affects Mitochondrial Transcription Factors Expression after Cerebral Ischemia in Rats

    Directory of Open Access Journals (Sweden)

    Yongshan Hu

    2012-02-01

    Full Text Available Increasing evidence shows that exercise training is neuroprotective after stroke, but the underlying mechanisms are unknown. To clarify this critical issue, the current study investigated the effects of early treadmill exercise on the expression of mitochondrial biogenesis factors. Adult rats were subjected to ischemia induced by middle cerebral artery occlusion followed by reperfusion. Expression of two genes critical for transcriptional regulation of mitochondrial biogenesis, peroxisome proliferator-activated receptor coactivator-1 (PGC-1 and nuclear respiratory factor-1 (NRF-1, were examined by RT-PCR after five days of exercise starting at 24 h after ischemia. Mitochondrial protein cytochrome C oxidase subunit IV (COX IV was detected by Western blot. Neurological status and cerebral infarct volume were evaluated as indices of brain damage. Treadmill training increased levels of PGC-1 and NRF-1 mRNA, indicating that exercise promotes rehabilitation after ischemia via regulation of mitochondrial biogenesis.

  13. Evolution of transcriptional networks in yeast: alternative teams of transcriptional factors for different species

    OpenAIRE

    Adriana Muñoz; Daniella Santos Muñoz; Aleksey Zimin; Yorke, James A.

    2016-01-01

    Background The diversity in eukaryotic life reflects a diversity in regulatory pathways. Nocedal and Johnson argue that the rewiring of gene regulatory networks is a major force for the diversity of life, that changes in regulation can create new species. Results We have created a method (based on our new “ping-pong algorithm) for detecting more complicated rewirings, where several transcription factors can substitute for one or more transcription factors in the regulation of a family of co-r...

  14. Maritime Education and Training Miscellaneous. Hearings Before the Ad Hoc Select Subcommittee on Maritime Education and Training of the Committee on Merchant Marine and Fisheries. House of Representatives, Ninety-Sixth Congress (December 6, 11, 1979, February 25, April 28, 1980).

    Science.gov (United States)

    Congress of the U.S., Washington, DC. House Committee on Merchant Marine and Fisheries.

    The three days of House subcommittee hearings reported in this transcript deal with the proposed Maritime Education and Training Act, H.R. 5451 and the Maritime Education and Training Elements of Maritime Appropriations Authorization Act--H.R. 6554. The primary goal of the legislation is to provide for the supply of merchant marine officers from…

  15. Training report of the FBR cycle training facility in 2001

    Energy Technology Data Exchange (ETDEWEB)

    Watanabe, Toshio; Sasaki, Kazuichi; Sawada, Makoto [Japan Nuclear Cycle Development Inst., Tsuruga, Fukui (Japan); Ozawa, Kazumasa [Hitachi Ltd., Tokyo (Japan); Nagai, Humio [Fast Technology Engineering Co. (Japan)

    2002-08-01

    The FBR Cycle Training Facility has been operating since Sep. 2000 for staff of FBR 'Monju' and ATR 'Fugen' and fireman of the local community, etc. In 2000, 6 courses of the sodium handling training were held 22 times and also eight courses of the maintenance training were 11 times and total participants were 305. In order to grade up the trainigs for providing restarting of 'Monju', the corresponding training course on sodium piping leakage accident was added as newly sodium handling training course and partial contents of some courses were devised based on some comments by the training discussing committee organized in our center. In 2001, seven sodium handling training courses were carried out 25 times and eight maintenance courses were conducted 11 times and total participated number was 220, i.e. 157 trainees for sodium training courses and 63 trainees for maintenance training courses. Additionally, since a new licensed sodium training course for sodium handling workers included head of worker will be introduced from next year owing to the fire accident of maintenance building in the experimental fast reactor 'JOYO' occurred Oct in 2001, which was coursed by sodium spontaneous combustion due to inefficient handling on tidy up of the sodium handling working, training curriculum, training text and questions for examination are provided as for preparing its course cooperated with Oarai Engineering Center. (author)

  16. Peace Corps Aquaculture Training Manual. Training Manual T0057.

    Science.gov (United States)

    Peace Corps, Washington, DC. Information Collection and Exchange Div.

    This Peace Corps training manual was developed from two existing manuals to provide a comprehensive training program in fish production for Peace Corps volunteers. The manual encompasses the essential elements of the University of Oklahoma program that has been training volunteers in aquaculture for 25 years. The 22 chapters of the manual are…

  17. The Efficacy of Stuttering Measurement Training: Evaluating Two Training Programs

    Science.gov (United States)

    Bainbridge, Lauren A.; Stavros, Candace; Ebrahimian, Mineh; Wang, Yuedong; Ingham, Roger J.

    2015-01-01

    Purpose: Two stuttering measurement training programs currently used for training clinicians were evaluated for their efficacy in improving the accuracy of total stuttering event counting. Method: Four groups, each with 12 randomly allocated participants, completed a pretest-posttest design training study. They were evaluated by their counts of…

  18. Training Directors' Perceptions about the Successful Implementation of Supervisory Training.

    Science.gov (United States)

    Taylor, Paul

    1992-01-01

    In a sample of 285 (of 1,363) corporations, reports of supervisor reaction to supervisory training were assessed to measure program success. Results highlight the importance of training administrators' role in convincing supervisors and their superiors of the value of training before implementation. (SK)

  19. Making Training Core Business: Enterprise Registered Training Organisations in Australia

    Science.gov (United States)

    Smith, Erica; Smith, Andy

    2009-01-01

    This paper examines the Australian phenomenon of Enterprise Registered Training Organizations (RTOs). These are organizations that do not have training as their main business but that are accredited to deliver training and award qualifications, primarily to their own workers. Although Enterprise RTOs have been in existence in one form or another…

  20. Adaptations to speed endurance training in highly trained soccer players

    DEFF Research Database (Denmark)

    Nyberg, Michael Permin; Fiorenza, Matteo; Lund, Anders

    2016-01-01

    PURPOSE: The present study examined whether a period of additional speed endurance training would improve intense intermittent exercise performance in highly trained soccer players during the season and whether the training changed aerobic metabolism and the level of oxidative enzymes in type I a...