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Sample records for bream sparus aurata

  1. Taurine supplemented plant protein based diets with alternative lipid sources for juvenile sea bream, sparus aurata

    Science.gov (United States)

    Two lipid sources were evaluated as fish oil replacements in fishmeal free, plant protein based diets for juvenile gilthead sea bream, Sparus aurata. A twelve week feeding study was undertaken to examine the performance of fish fed the diets with different sources of essential fatty acids (canola o...

  2. Visualizing mineralization in deformed opercular bones of larval gilthead sea bream (Sparus aurata)

    OpenAIRE

    Morel, Catherine; Adriaens, Dominique; Boone, Matthieu; Wolf, T.; Van Hoorebeke, Luc; Sorgeloos, Patrick

    2010-01-01

    During the rearing process of gilthead sea bream (Sparus aurata), abnormal development of the opercular bone is particularly common (Aquaculture 156, 1997, 165). In order to alleviate its occurrence in rearing facilities, it's crucial to identify the very first physical signs of deviation in normal skeletal development. Nano-CT-scanning was tested for its applicability to quantify deviations in bone mineralization levels. Seven opercles were dissected from larvi of 65 days post hatching, rand...

  3. Season and size effects: changes in the quality of gilthead sea bream (Sparus aurata L.

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    R. RODRIGUEZ

    2012-12-01

    Full Text Available The aim of this study was to investigate the potential effects of the harvesting season and fish size on the final quality of cage farmed gilthead sea bream (Sparus aurata. Morphometric analysis, fat stores estimation, fillet composition analysis, fillet fatty acids profile determination and sensory analysis were carried out on a commercially-sized cage farmed gilthead sea bream (Sparus aurata L. population in April, June, July and November. Both season and size had a significant effect in morphometric characteristics. The most pronounced size effect was the change of fish shape, becoming rounder with fish growth, the increase of filleting yield and condition index. An increase of both peritoneal and perivisceral fat was observed in summer months. In general size had little effect on muscle composition. A significant size effect was observed in the fillet lipid content, which increased with it. Fillet lipid content was unexpected lower in the warm period (June-July. This can be possibly attributed to feeding restrictions that may have occurred, due to anoxic incidents related to high temperatures.Slight seasonal differentiations occurred in the muscle fatty acids. No significant differences were found in the organoleptic characteristics of the two size groups compared.

  4. Histopathology of cultured sea bream Sparus aurata infected with sanguinicolid trematodes.

    Science.gov (United States)

    Padrós, F; Zarza, C; Crespo, S

    2001-01-26

    The present study is the first report of a sanguinicolid infection affecting sea bream Sparus aurata cultured in net cages in the NE of Spain. The disease was associated with trickling mortalities during the cold season (1999 and 2000). Examination of gill wet mounts of the affected population revealed that sanguinicolid infection was present in 82.6 and 100% of the fish sampled in 1999 and 2000, respectively. Adult flukes, which were located in the kidney, were tentatively identified as members of the family Sanguinicolidae, subfamily Cardicolinae. Eggs and miracidia were found in the gill vascular structures. The inflammatory response triggered by the parasites was moderate and the lesions caused by either eggs and miracidia in the gills or adult flukes in the kidney were not extremely severe, possibly because of the moderate intensity of the parasitosis. Histological observations of sanguinicolid infected sea bream presented here are compared with those reported in other fish species. The role played on sea bream morbility and mortality by other factors (occurrence of a simultaneous moderate monogenean infection, immunological impairement related to low water temperatures) is discussed. PMID:11253874

  5. Cloning, expression, and tissue localisation of prolactin in adult sea bream (Sparus aurata).

    Science.gov (United States)

    Santos, C R; Brinca, L; Ingleton, P M; Power, D M

    1999-04-01

    A major action of prolactin (PRL) in teleost fish is the maintenance of hydromineral balance in euryhaline species in fresh water. The function of PRL in marine teleosts is less certain and unlike euryhaline teleosts, such as tilapia and salmon, there is relatively little information about protein or gene structure. Associated with studies to determine potential functions of PRL, pituitary prolactin cDNA has been cloned and sequenced from sea bream (Sparus aurata), a marine teleost. The sequence obtained spanned 1349 bp and contained an open reading frame encoding a protein of 212 amino acids composed of a putative signal peptide of 24 residues and a mature protein of 188 amino acids. N-terminal sequencing of the native protein confirmed unambiguously the cleavage site, Ala24, Val25, predicted from alignments of the sea bream PRL cDNA with that of other teleosts. The presence of only one form of PRL in sea bream was supported by identification using Northern blots of only a single transcript of 1.35 kb. Reverse transcription and polymerase chain reaction techniques coupled with Southern blot analysis resulted in the detection of PRL in the pituitary but also in the intestine, liver, ovary, and testes. PMID:10094859

  6. A Comparative BAC Map for the Gilthead Sea Bream (Sparus aurata L.

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    Heiner Kuhl

    2011-01-01

    Full Text Available This study presents the first comparative BAC map of the gilthead sea bream (Sparus aurata, a highly valuated marine aquaculture fish species in the Mediterranean. High-throughput end sequencing of a BAC library yielded 92,468 reads (60.6 Mbp. Comparative mapping was achieved by anchoring BAC end sequences to the three-spined stickleback (Gasterosteus aculeatus genome. BACs that were consistently ordered along the stickleback chromosomes accounted for 14,265 clones. A fraction of 5,249 BACs constituted a minimal tiling path that covers 73.5% of the stickleback chromosomes and 70.2% of the genes that have been annotated. The N50 size of 1,485 “BACtigs” consisting of redundant BACs is 337,253 bp. The largest BACtig covers 2.15 Mbp in the stickleback genome. According to the insert size distribution of mapped BACs the sea bream genome is 1.71-fold larger than the stickleback genome. These results represent a valuable tool to researchers in the field and may support future projects to elucidate the whole sea bream genome.

  7. Visualizing mineralization in deformed opercular bones of larval gilthead sea bream (Sparus aurata)

    OpenAIRE

    Morel, C.; Adriaens, D.; Boone, M.; Wolf, T.; Van Hoorebeke, L.; Sorgeloos, P.

    2010-01-01

    P>During the rearing process of gilthead sea bream (Sparus aurata), abnormal development of the opercular bone is particularly common (Aquaculture 156, 1997, 165). In order to alleviate its occurrence in rearing facilities, it's crucial to identify the very first physical signs of deviation in normal skeletal development. Nano-CT-scanning was tested for its applicability to quantify deviations in bone mineralization levels. Seven opercles were dissected from larvi of 65 days post hatching, ra...

  8. Cloning, characterization, and tissue distribution of prolactin receptor in the sea bream (Sparus aurata).

    Science.gov (United States)

    Santos, C R; Ingleton, P M; Cavaco, J E; Kelly, P A; Edery, M; Power, D M

    2001-01-01

    The prolactin receptor (PRLR) was cloned and its tissue distribution characterized in adults of the protandrous hermaphrodite marine teleost, the sea bream (Sparus aurata). An homologous cDNA probe for sea bream PRLR (sbPRLR) was obtained by RT-PCR using gill mRNA. This probe was used to screen intestine and kidney cDNA libraries from which two overlapping clones (1100 and 2425 bp, respectively) were obtained. These clones had 100% sequence identity in the overlapping region (893 bp) and were used to deduce the complete amino acid sequence of sbPRLR. The receptor spans 2640 bp and encodes a protein of 537 amino acids. Features characteristic of PRLR, two pairs of cysteines, WS box, hydrophobic transmembrane domain, box 1, and box 2, were identified and showed a high degree of sequence identity to PRLRs from other vertebrate species. SbPRLR is 29 and 32% identical to tilapia (Oreochromis niloticus) and goldfish (Carassius auratus) PRLRs, respectively. In the sea bream two PRLR transcripts of 2.8 and 3.2 kb were detected in the intestine, kidney, and gills and a single transcript of 2.8 kb was detected in skin and pituitary by Northern blot. Spermiating gonads (more than 95% male tissue; gonado-somatic index of 0.6) contained, in addition to the 2.8-kb transcript, three more transcripts of 1.9, 1.3, and 1.1 kb. RT-PCR, which is a far more sensitive method than Northern blot, detected PRLR mRNA in gills, intestine, brain, pituitary, kidney, liver, gonads, spleen, head-kidney, heart, muscle, and bone. Immunohistochemistry using specific polyclonal antibodies raised against an oligopeptide from the extracellular domain of sbPRLR detected PRLR in several epithelial tissues of juvenile sea bream, including the anterior gut, renal tubule, choroid membrane of the third ventricle, saccus vasculosus, branchial chloride cells, and branchial cartilage. PMID:11161768

  9. Fast skeletal muscle transcriptome of the Gilthead sea bream (Sparus aurata determined by next generation sequencing

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    Garcia de la serrana Daniel

    2012-05-01

    Full Text Available Abstract Background The gilthead sea bream (Sparus aurata L. occurs around the Mediterranean and along Eastern Atlantic coasts from Great Britain to Senegal. It is tolerant of a wide range of temperatures and salinities and is often found in brackish coastal lagoons and estuarine areas, particularly early in its life cycle. Gilthead sea bream are extensively cultivated in the Mediterranean with an annual production of 125,000 metric tonnes. Here we present a de novo assembly of the fast skeletal muscle transcriptome of gilthead sea bream using 454 reads and identify gene paralogues, splice variants and microsatellite repeats. An annotated transcriptome of the skeletal muscle will facilitate understanding of the genetic and molecular basis of traits linked to production in this economically important species. Results Around 2.7 million reads of mRNA sequence data were generated from the fast myotomal of adult fish (~2 kg and juvenile fish (~0.09 kg that had been either fed to satiation, fasted for 3-5d or transferred to low (11°C or high (33°C temperatures for 3-5d. Newbler v2.5 assembly resulted in 43,461 isotigs >100 bp. The number of sequences annotated by searching protein and gene ontology databases was 10,465. The average coverage of the annotated isotigs was x40 containing 5655 unique gene IDs and 785 full-length cDNAs coding for proteins containing 58–1536 amino acids. The v2.5 assembly was found to be of good quality based on validation using 200 full-length cDNAs from GenBank. Annotated isotigs from the reference transcriptome were attributable to 344 KEGG pathway maps. We identified 26 gene paralogues (20 of them teleost-specific and 43 splice variants, of which 12 had functional domains missing that were likely to affect their biological function. Many key transcription factors, signaling molecules and structural proteins necessary for myogenesis and muscle growth have been identified. Physiological status affected the

  10. Nutritional regulation of glucose-6-phosphatase gene expression in liver of the gilthead sea bream (Sparus aurata)

    OpenAIRE

    Caseras Surribas, Anna; Metón Teijeiro, Isidoro; Vives, C.; Egea Liria, Miriam; Fernández González, Felipe Javier; Vázquez Baanante, Ma. Isabel

    2002-01-01

    To examine the role of glucose-6-phosphatase (G6Pase) in glucose homeostasis in the diabeteslike experimental model of carnivorous fish, we analysed postprandial variations and the effect of starvation, ration size and diet composition on the regulation of G6Pase expression at the enzyme activity and mRNA level in the liver of gilthead sea bream (Sparus aurata ). G6Pase expression increased in long-term starved or energy-restricted fish. In contrast to data reported for other fish species, sh...

  11. Characterization of the cellular damage induced by Aflatoxin B1 in sea bream (Sparus aurata Linnaeus, 1758 hepatocytes

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    Giuseppe Crescenzo

    2010-01-01

    Full Text Available Gilthead sea bream (Sparus aurata L. is one of the most intensively farmed fish spe- cies in the Mediterranean, greatly studied for the relevant economic value, although its sensitivity to Aflatoxin B1 (AFB1 has to be investigated, yet. The aim of this study was to perform an in vitro evalua- tion of cytotoxic potential of AFB1 on S. aurata hepatocytes in order to grade the range of AFB1 toxicity, and the boundary between acute and long-term toxicity. Primary monolayer cultures of hepatocytes from S. aurata juveniles were treated with a wide range of concentrations from 5x103 ng/ml to 2x10 2x10-5 ng/ml of AFB1 for a different period of exposure (24, 48, 72 hours. The cytotoxic activity was characterized by MTT reduction assay. After each exposition hepatocytes were examined for morphologic alterations and apoptosis induction. AFB1 exposure significantly reduced cell viability in a dose- and time-depend- ent manner. Dose-response curves obtained after 24, 48 and 72 hrs revealed that prolonged exposure times lead to a significant increase of the toxicpotencyofAFB toxic potency of AFB AFB1. Ourresultsdemonstratethat Our results demonstrate that S. aurata hepatocytes are highly sensitive to AFB1 exposure. Such scientific findings could provide new insights to investigate the real impact of aflatoxin on marine farmed fish.

  12. Epitheliocystis agents in sea bream Sparus aurata: morphological evidence for two distinct chlamydia-like developmental cycles.

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    Crespo, S; Zarza, C; Padrós, F; Marín de Mateo, M

    1999-06-23

    The morphology of membrane-bound intracellular inclusions, or 'cysts', of epitheliocystis from sea bream Sparus aurata is described. Inclusions under the light microscope appear either granular or amorphous. Granular inclusions do not elicit a proliferative host reaction and contain the 3 distinctive developmental stages of chlamydial organisms: the highly pleomorphic reproductive form or reticulate body, the condensing form or intermediate body and the infective non-dividing rather uniform elementary body. Amorphous inclusions may elicit a proliferative host reaction and contain prokaryotic organisms which differ morphologically from those reported within granular cysts. More or less elongated electron-lucent organisms divide by fission to give rise to electron-dense non-dividing small cells with a dense nucleoid. Vacuolated and non-vacuolated small cells are reported. The morphology and developmental cycle of sea bream epitheliocystis agents would support their chlamydial nature; however, the immunohistochemical study conducted on gill samples which carried both inclusions failed to demonstrate the expression of lipopolysaccharide (LPS) chlamydial antigen. The different stages of the 2 distinct developmental cycles described in the present study are compared with electron microscope observations of epitheliocystis organisms reported from different host species. The hypothesis that epitheliocystis infection in the sea bream might be caused by a unique highly pleomorphic chlamydia-like agent, the life history of which includes 2 entirely different developmental cycles, is discussed. PMID:10439904

  13. Influence of the diet on the microbial diversity of faecal and gastrointestinal contents in gilthead sea bream (Sparus aurata) and intestinal contents in goldfish (Carassius auratus)

    OpenAIRE

    Silva, Flavia Cristina De Paula; Nicoli, Jacques Robert; Zambonino, Jose Luiz; Kaushik, Sadasivam; Gatesoupe, François-Joel

    2011-01-01

    Fish intestinal microbiota changes with diet and this effect is of particular interest considering the increasing substitution of fish meal by plant protein sources. The objective of this work was to study the effects of partial substitution of fish meal with lupin and rapeseed meals on gut microbiota of the gilthead sea bream (Sparus aurata) and in goldfish (Carassius auratus). Faecal, gastrointestinal and intestinal contents were characterized by culture-based and molecular methods. Vibrion...

  14. Impact of Fishmeal Replacement in Diets for Gilthead Sea Bream (Sparus aurata) on the Gastrointestinal Microbiota Determined by Pyrosequencing the 16S rRNA Gene

    OpenAIRE

    Estruch, Guillem; COLLADO AMORES, MARÍA CARMEN; Peñaranda, D.S.; Tomas-Vidal, A.; Jover Cerda, Miguel; Pérez-Martínez, G; Martínez-Llorens, Silvia

    2015-01-01

    [EN] Recent studies have demonstrated the impact of diet on microbiota composition, but the essential need for the optimization of production rates and costs forces farms and aquaculture production to carry out continuous dietary tests. In order to understand the effect of total fishmeal replacement by vegetable-based feed in the sea bream (Sparus aurata), the microbial composition of the stomach, foregut, midgut and hindgut was analysed using high-throughput 16S rDNA sequ...

  15. Comparison among Different Gilthead Sea Bream (Sparus aurata) Farming Systems: Activity of Intestinal and Hepatic Enzymes and 13C-NMR Analysis of Lipids

    OpenAIRE

    Vincenzo Zonno; Francesco Paolo Fanizzi; Carlo Storelli; Giorgia Bressani; Pascali, Sandra A. De; Laura Del Coco; Paride Papadia

    2009-01-01

    In order to evaluate differences in general health and nutritional values of gilthead sea bream (Sparus aurata), the effects of semi-intensive, land-based tanks and sea-cages intensive rearing systems were investigated, and results compared with captured wild fish. The physiological state was determined by measuring the activity of three different intestinal digestive enzymes: alkaline phosphatase (ALP), leucine aminopeptidase (LAP) and maltase; and the activity of the hepatic ALP. Also, the ...

  16. Exposure of sea bream (Sparus aurata) to toxic concentrations of benzo[a]pyrene: possible human health effect.

    Science.gov (United States)

    Zena, R; Speciale, A; Calabrò, C; Calò, M; Palombieri, D; Saija, A; Cimino, F; Trombetta, D; Lo Cascio, P

    2015-12-01

    Polycyclic aromatic hydrocarbons (PAHs) can accumulate in the food chain, due to their lipophilic properties. Fish can accumulate contaminants including PAHs and frequent consumption of such contaminated fish can pose risk to human health. The aim of this study was to clarify if acute exposure of sea bream (Sparus aurata, a fish species of great economic importance in the Atlantic and Mediterranean areas) to a PAH, benzo[a]pyrene (B[a]P), at a dose that can induce CYP1A and pathological changes in fish gills, liver and muscle, can induce accumulation in muscle. We investigated the cytotoxic effects (as changes in cell viability, DNA laddering and glutathione content) of in vitro exposure of human peripheral blood mononuclear cells (PBMCs) to organic extracts obtained from muscle of sea breams previously exposed via water to B[a]P (2mg/l, for 12, 24 and 72 h). At this level of exposure, B[a]P caused morphological changes, inflammatory response and CYP1A induction not only in sea bream gills and liver but also in muscle; furthermore, in fish muscle we observed a substantial B[a]P accumulation, which may be associated with the increased CYP1A activity in liver and especially in muscle. However, when PBMCs were exposed to organic extracts obtained from sea bream muscle contaminated with B[a]P, a toxic, although modest effect was revealed, consisting in a significant decrease in cell glutathione levels without alterations in cell viability and DNA laddering. This suggests that consumption of sea breams from B[a]P contaminated waters might represent a risk for human health. PMID:26232038

  17. A gene-based radiation hybrid map of the gilthead sea bream Sparus aurata refines and exploits conserved synteny with Tetraodon nigroviridis

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    Tsalavouta Matina

    2007-02-01

    Full Text Available Abstract Background Comparative teleost studies are of great interest since they are important in aquaculture and in evolutionary issues. Comparing genomes of fully sequenced model fish species with those of farmed fish species through comparative mapping offers shortcuts for quantitative trait loci (QTL detections and for studying genome evolution through the identification of regions of conserved synteny in teleosts. Here a comparative mapping study is presented by radiation hybrid (RH mapping genes of the gilthead sea bream Sparus aurata, a non-model teleost fish of commercial and evolutionary interest, as it represents the worldwide distributed species-rich family of Sparidae. Results An additional 74 microsatellite markers and 428 gene-based markers appropriate for comparative mapping studies were mapped on the existing RH map of Sparus aurata. The anchoring of the RH map to the genetic linkage map resulted in 24 groups matching the karyotype of Sparus aurata. Homologous sequences to Tetraodon were identified for 301 of the gene-based markers positioned on the RH map of Sparus aurata. Comparison between Sparus aurata RH groups and Tetraodon chromosomes (karyotype of Tetraodon consists of 21 chromosomes in this study reveals an unambiguous one-to-one relationship suggesting that three Tetraodon chromosomes correspond to six Sparus aurata radiation hybrid groups. The exploitation of this conserved synteny relationship is furthermore demonstrated by in silico mapping of gilthead sea bream expressed sequence tags (EST that give a significant similarity hit to Tetraodon. Conclusion The addition of primarily gene-based markers increased substantially the density of the existing RH map and facilitated comparative analysis. The anchoring of this gene-based radiation hybrid map to the genome maps of model species broadened the pool of candidate genes that mainly control growth, disease resistance, sex determination and reversal, reproduction as well

  18. Mast cells in the intestine and gills of the sea bream, Sparus aurata, exposed to a polychlorinated biphenyl, PCB 126.

    Science.gov (United States)

    Lauriano, Eugenia Rita; Calò, Margherita; Silvestri, Giuseppa; Zaccone, Daniele; Pergolizzi, Simona; Lo Cascio, Patrizia

    2012-02-01

    The presence of mast cells has been reported in all classes of vertebrates, including many teleost fish families. The mast cells of teleosts, both morphologically and functionally, show a close similarity to the mast cells of mammals. Mast cells of teleosts, localized in the vicinity of blood vessels of the intestine, gills and skin, may play an important role in the mechanisms of inflammatory response, because they express a number of functional proteins, including piscidins, which are antimicrobical peptides that act against a broad-spectrum of pathogens. An increase in the number of mast cells in various tissues and organs of teleosts seems to be linked to a wide range of stressful conditions, such as exposure to heavy metals (cadmium, copper, lead and mercury), exposure to herbicides and parasitic infections. This study analyzed the morphological localization and abundance of mast cells in the intestine and gills of sea bream, Sparus aurata, after a 12, 24 or 72 h exposure to PCB 126, a polychlorinated biphenyl, which is a potent immunotoxic agent. In the organs of fish exposed to PCB 126, it was observed that in addition to congestion of blood vessels, there was extravasation of red blood cells, infiltration of lymphocytes, and a progressive increase in numbers of mast cells. These data confirm the immunotoxic action of PCB, and the involvement of mast cells in the inflammatory response. PMID:21565388

  19. Assessment of freshness and freeze-thawing of sea bream fillets (Sparus aurata) by a cytosolic enzyme: Lactate dehydrogenase.

    Science.gov (United States)

    Diop, Mamadou; Watier, Denis; Masson, Pierre-Yves; Diouf, Amadou; Amara, Rachid; Grard, Thierry; Lencel, Philippe

    2016-11-01

    The evaluation of freshness and freeze-thawing of fish fillets was carried out by assessment of autolysis of cells using a cytosolic enzyme lactate dehydrogenase. Autolysis plays an important role in spoilage of fish and postmortem changes in fish tissue are due to the breakdown of the cellular structures and release of cytoplasmic contents. The outflow of a cytosolic enzyme, lactate dehydrogenase, was studied in sea bream fillets and the Sparus aurata fibroblasts (SAF-1) cell-line during an 8day storage period at +4°C. A significant increase of lactate dehydrogenase release was observed, especially after 5days of storage. The ratio between the free and the total lactate dehydrogenase activity is a promising predictive marker to measure the quality of fresh fish fillets. The effect of freeze-thawing on cytosolic lactate dehydrogenase and lysosomal α-d-glucosidase activities was also tested. Despite the protecting effect of the tissue compared to the cell-line, a loss of lactate dehydrogenase activity, but not of α-d-glucosidase, was observed. In conclusion, lactate dehydrogenase may be used as a marker to both assess freshness of fish and distinguish between fresh and frozen-thawed fish fillets. PMID:27211667

  20. Sample size matters in dietary gene expression studies—A case study in the gilthead sea bream (Sparus aurata L.

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    Fotini Kokou

    2016-05-01

    Full Text Available One of the main concerns in gene expression studies is the calculation of statistical significance which in most cases remains low due to limited sample size. Increasing biological replicates translates into more effective gains in power which, especially in nutritional experiments, is of great importance as individual variation of growth performance parameters and feed conversion is high. The present study investigates in the gilthead sea bream Sparus aurata, one of the most important Mediterranean aquaculture species. For 24 gilthead sea bream individuals (biological replicates the effects of gradual substitution of fish meal by plant ingredients (0% (control, 25%, 50% and 75% in the diets were studied by looking at expression levels of four immune-and stress-related genes in intestine, head kidney and liver. The present results showed that only the lowest substitution percentage is tolerated and that liver is the most sensitive tissue to detect gene expression variations in relation to fish meal substituted diets. Additionally the usage of three independent biological replicates were evaluated by calculating the averages of all possible triplets in order to assess the suitability of selected genes for stress indication as well as the impact of the experimental set up, thus in the present work the impact of FM substitution. Gene expression was altered depending of the selected biological triplicate. Only for two genes in liver (hsp70 and tgf significant differential expression was assured independently of the triplicates used. These results underlined the importance of choosing the adequate sample number especially when significant, but minor differences in gene expression levels are observed.

  1. Grow-Out Of Sea Bream Sparus aurata in Turkey, Particularly in a Land-Based Farm With Recirculation System in Çanakkale: Better Use of Water, Nutrients and Space

    OpenAIRE

    , Erdem Ökte

    2002-01-01

    Gilthead sea bream (Sparus aurata) is being farmed in Turkey mostly in cages in the Aegean coast from Çe?me to Fethiye. There is also a new facility that utilizes land-based re-circulation technology in Çanakkale. Stocking densities, growth, maturation and FCR, water quality, diseases and management are examined in the different types of production techniques.

  2. Use of microarray technology to assess the time course of liver stress response after confinement exposure in gilthead sea bream (Sparus aurata L.

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    Cairns Michael T

    2010-03-01

    Full Text Available Abstract Background Selection programs for growth and stress traits in cultured fish are fundamental to the improvement of aquaculture production. The gilthead sea bream (Sparus aurata is the main aquacultured species in the Mediterranean area and there is considerable interest in the genetic improvement of this species. With the aim of increasing the genomic resources in gilthead sea bream and identifying genes and mechanisms underlying the physiology of the stress response, we developed a cDNA microarray for gilthead sea bream that is enriched by suppression substractive hybridization with stress and immunorelevant genes. This microarray is used to analyze the dynamics of gilthead sea bream liver expression profile after confinement exposure. Results Groups of confined and control juvenile fish were sampled at 6, 24, 72 and 120 h post exposure. GeneSpring analyses identified 202 annotated genes that appeared differentially expressed at least at one sampling time (P Conclusions Collectively, these findings show the complex nature of the adaptive stress response with a clear indication that the ER is an important control point for homeostatic adjustments. The study also identifies metabolic pathways which could be analyzed in greater detail to provide new insights regarding the transcriptional regulation of the stress response in fish.

  3. Cultivation of gilthead sea bream (Sparus aurata Linnaeus, 1758 in low salinity inland brackish geothermal water

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    Samuel Appelbaum

    2009-04-01

    Full Text Available In the course of attempting to culture gilthead sea bream in inland brackish geothermal water, indoor and outdoor research studies have been ongoing from early 2006 at the "Bengis Centre for Desert Aquaculture" of the Institutes for Desert Research in Israel, some of which are described below. Gilthead sea bream fingerlings with an average weight of 19 g were acclimated to and reared for 56 days in brackish water of three salinities: 2.5, 3.5 and 4.5 ppt collected from three different locations. At the salinity of 3.5 ppt the fish grew best (P Artemia nauplii plus larval dry feed and were grown in brackish water of 2.6 ppt salinity for 8 weeks reaching a weight gain of 92% at a survival rate of 83%. The control groups at sea water (39 ppt reached at the same time a weight gain of 95% at a survival rate of 98%. In a further study gilthead sea bream juveniles with an average weight of 2.24 g were reared in brackish water with a salinity of 3.6 ppt for 8 weeks and received salt added diets of 4% and 6%. Compared with the control diet with no salt added, both salt rich diets significantly improved the fingerlings growth and survival rates as well as the feed conversion ratio. The 6% salt rich diet promoted fish weight gain by 560% which was the best performance (P <0.05 followed by 448% weight gain with the 4% salt rich diet while the lowest performance with a weight gain of only 360% was obtained with the control (no salt added diet. The next study with higher levels of salt added diets showed that gilthead sea bream post larvae with an average weight of 0.58 g reared in brackish geothermal water of 2.9 ppt salinity for 10 weeks grew best (P < 0.05 obtaining a weight gain of 778% with the highest survival rate of 88% when fed a diet containing 12% salt. In the studies with the salt rich diets, the salt incorporated was obtained by the evaporation of brine produced during the process of desalination of the brackish geothermal water from the same

  4. Comparison among Different Gilthead Sea Bream (Sparus aurata Farming Systems: Activity of Intestinal and Hepatic Enzymes and 13C-NMR Analysis of Lipids

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    Vincenzo Zonno

    2009-12-01

    Full Text Available In order to evaluate differences in general health and nutritional values of gilthead sea bream (Sparus aurata, the effects of semi-intensive, land-based tanks and sea-cages intensive rearing systems were investigated, and results compared with captured wild fish. The physiological state was determined by measuring the activity of three different intestinal digestive enzymes: alkaline phosphatase (ALP, leucine aminopeptidase (LAP and maltase; and the activity of the hepatic ALP. Also, the hepatic content in protein, cholesterol, and lipid were assessed. 13C-NMR analysis for qualitative and quantitative characterization of the lipid fraction extracted from fish muscles for semiintensive and land based tanks intensive systems was performed. The lipid fraction composition showed small but significant differences in the monounsaturated/saturated fatty acid ratio, with the semi-intensive characterized by higher monounsaturated and lower saturated fatty acid content with respect to land based tanks intensive rearing system.

  5. Distribution and depletion of sulfadiazine after a multiple per os dosing in gilthead sea bream (Sparus aurata fed two different diets

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    G. RIGOS

    2013-07-01

    Full Text Available The distribution and depletion profile of sulfadiazine (SDZ were investigated in gilthead sea bream (Sparus aurata fed on fish oil (FO or plant oil-based (PO diets. Fish averaging 230 g were given medicated feed containing 25 mg SDZ kg-1 fish for 5 days at 24-26oC. Blood and muscle plus skin were sampled on days 1, 3, 5, 6, 8 and 9.  Differences in plasma and fillet SDZ levels between the two groups were statistically insignificant. The maximum drug concentrations in plasma were 3.2 ± 1.9 μg mL-1 and 2.9 ± 1.2 μg mL-1 in the PO and FO groups, respectively. In post-medicated samples depletion rapidly reached concentrations close to the level of quantification at 72 h post medication. Withdrawal times to reach consumer safety levels were calculated to be 103 and 118 h for the FO and the PO groups, respectively. N4-acetylation was found to be the major metabolic pathway of SDZ in gilthead sea bream fillet accounting for 23 and 19% of the parent compound in the FO and the PO groups, respectively. Overall, alteration of the dietary lipid profile induced insignificant effects on the kinetics of SDZ. The high tissue SDZ levels during medication and the fast removal of the parent compound and its metabolites from edible tissues of gilthead sea bream reflect a promising antibacterial profile.

  6. Effects of dietary nutrient composition on de novo lipogenesis in gilthead sea bream (Sparus aurata)

    DEFF Research Database (Denmark)

    Ekmann, Kim Schøn

    far, there are no indications that differences in nutrient digestibility coefficients can explain these differences, since gilthead sea bream largely digests dietary nutrients similarly or better than salmonids. As dietary nutrients upon digestion can be endogenously converted into other nutrients or...... metabolites, it can be hard to quantitatively conclude on the fate of them. Using stable isotope tracers (such as 13C labelled starch or protein) allows us to trace specific nutrients and determine to which extent they are endogenously converted into other metabolites. The present thesis comprises three...... supporting papers which look into the conversion of dietary starch and protein into body lipids as well as the consequences of this on fatty acid profile of the fish. Results from paper I showed that between 4.2 and 8.4% of digested starch was converted into body lipids de novo, corresponding to a synthesis...

  7. Lysine and Leucine Deficiencies Affect Myocytes Development and IGF Signaling in Gilthead Sea Bream (Sparus aurata.

    Directory of Open Access Journals (Sweden)

    Sheida Azizi

    Full Text Available Optimizing aquaculture production requires better knowledge of growth regulation and improvement in diet formulation. A great effort has been made to replace fish meal for plant protein sources in aquafeeds, making necessary the supplementation of such diets with crystalline amino acids (AA to cover the nutritional requirements of each species. Lysine and Leucine are limiting essential AA in fish, and it has been demonstrated that supplementation with them improves growth in different species. However, the specific effects of AA deficiencies in myogenesis are completely unknown and have only been studied at the level of hepatic metabolism. It is well-known that the TOR pathway integrates the nutritional and hormonal signals to regulate protein synthesis and cell proliferation, to finally control muscle growth, a process also coordinated by the expression of myogenic regulatory factors (MRFs. This study aimed to provide new information on the impact of Lysine and Leucine deficiencies in gilthead sea bream cultured myocytes examining their development and the response of insulin-like growth factors (IGFs, MRFs, as well as key molecules involved in muscle growth regulation like TOR. Leucine deficiency did not cause significant differences in most of the molecules analyzed, whereas Lysine deficiency appeared crucial in IGFs regulation, decreasing significantly IGF-I, IGF-II and IGF-IRb mRNA levels. This treatment also down-regulated the gene expression of different MRFs, including Myf5, Myogenin and MyoD2. These changes were also corroborated by a significant decrease in proliferation and differentiation markers in the Lysine-deficient treatment. Moreover, both Lysine and Leucine limitation induced a significant down-regulation in FOXO3 gene expression, which deserves further investigation. We believe that these results will be relevant for the production of a species as appreciated for human consumption as it is gilthead sea bream and demonstrates

  8. Lysine and Leucine Deficiencies Affect Myocytes Development and IGF Signaling in Gilthead Sea Bream (Sparus aurata)

    Science.gov (United States)

    Azizi, Sheida; Nematollahi, Mohammad Ali; Mojazi Amiri, Bagher; Vélez, Emilio J.; Lutfi, Esmail; Navarro, Isabel; Capilla, Encarnación; Gutiérrez, Joaquim

    2016-01-01

    Optimizing aquaculture production requires better knowledge of growth regulation and improvement in diet formulation. A great effort has been made to replace fish meal for plant protein sources in aquafeeds, making necessary the supplementation of such diets with crystalline amino acids (AA) to cover the nutritional requirements of each species. Lysine and Leucine are limiting essential AA in fish, and it has been demonstrated that supplementation with them improves growth in different species. However, the specific effects of AA deficiencies in myogenesis are completely unknown and have only been studied at the level of hepatic metabolism. It is well-known that the TOR pathway integrates the nutritional and hormonal signals to regulate protein synthesis and cell proliferation, to finally control muscle growth, a process also coordinated by the expression of myogenic regulatory factors (MRFs). This study aimed to provide new information on the impact of Lysine and Leucine deficiencies in gilthead sea bream cultured myocytes examining their development and the response of insulin-like growth factors (IGFs), MRFs, as well as key molecules involved in muscle growth regulation like TOR. Leucine deficiency did not cause significant differences in most of the molecules analyzed, whereas Lysine deficiency appeared crucial in IGFs regulation, decreasing significantly IGF-I, IGF-II and IGF-IRb mRNA levels. This treatment also down-regulated the gene expression of different MRFs, including Myf5, Myogenin and MyoD2. These changes were also corroborated by a significant decrease in proliferation and differentiation markers in the Lysine-deficient treatment. Moreover, both Lysine and Leucine limitation induced a significant down-regulation in FOXO3 gene expression, which deserves further investigation. We believe that these results will be relevant for the production of a species as appreciated for human consumption as it is gilthead sea bream and demonstrates the importance of

  9. Intraspecific variation in aerobic and anaerobic locomotion: gilthead sea bream (Sparus aurata) and Trinidadian guppy (Poecilia reticulata) do not exhibit a trade-off between maximum sustained swimming speed and minimum cost of transport

    OpenAIRE

    Jon C Svendsen; Tirsgaard, Bjørn; Cordero, Gerardo A.; Steffensen, John F.

    2015-01-01

    Intraspecific variation and trade-off in aerobic and anaerobic traits remain poorly understood in aquatic locomotion. Using gilthead sea bream (Sparus aurata) and Trinidadian guppy (Poecilia reticulata), both axial swimmers, this study tested four hypotheses: (1) gait transition from steady to unsteady (i.e., burst-assisted) swimming is associated with anaerobic metabolism evidenced as excess post exercise oxygen consumption (EPOC); (2) variation in swimming performance (critical swimming spe...

  10. Quantitative trait loci involved in sex determination and body growth in the gilthead sea bream (Sparus aurata L. through targeted genome scan.

    Directory of Open Access Journals (Sweden)

    Dimitrios Loukovitis

    Full Text Available Among vertebrates, teleost fish exhibit a considerably wide range of sex determination patterns that may be influenced by extrinsic parameters. However even for model fish species like the zebrafish Danio rerio the precise mechanisms involved in primary sex determination have not been studied extensively. The zebrafish, a gonochoristic species, is lacking discernible sex chromosomes and the sex of juvenile fish is difficult to determine. Sequential protandrous hermaphrodite species provide distinct determination of the gender and allow studying the sex determination process by looking at the mechanism of sex reversal. This is the first attempt to understand the genetic basis of phenotypic variation for sex determination and body weight in a sequential protandrous hermaphrodite species, the gilthead sea bream (Sparus aurata. This work demonstrates a fast and efficient strategy for Quantitative Trait Loci (QTL detection in the gilthead sea bream, a non-model but target hermaphrodite fish species. Therefore a comparative mapping approach was performed to query syntenies against two other Perciformes, the European sea bass (Dicentrarchus labrax, a gonochoristic species and the Asian sea bass (Lates calcarifer a protandrous hermaphrodite. In this manner two significant QTLs, one QTL affecting both body weight and sex and one QTL affecting sex, were detected on the same linkage group. The co-segregation of the two QTLs provides a genomic base to the observed genetic correlation between these two traits in sea bream as well as in other teleosts. The identification of QTLs linked to sex reversal and growth, will contribute significantly to a better understanding of the complex nature of sex determination in S. aurata where most individuals reverse to the female sex at the age of two years through development and maturation of the ovarian portion of the gonad and regression of the testicular area. [Genomic sequences reported in this manuscript have been

  11. Molecular characterization of a gilthead sea bream (Sparus aurata) muscle tissue cDNA for carnitine palmitoyltransferase 1B (CPT1B).

    Science.gov (United States)

    Boukouvala, Evridiki; Leaver, Michael J; Favre-Krey, Laurence; Theodoridou, Maria; Krey, Grigorios

    2010-10-01

    Understanding the control of piscine fatty acid metabolism is important for determining the nutritional requirements of fish, and hence for the production of optimal aquaculture diets. The regulation and expression of carnitine palmitoyltransferase 1 (CPT1; EC No 2.3.1.21) are critical processes in the control of fatty acid metabolism, and here we report a cDNA from gilthead sea bream (Sparus aurata) which encodes a protein with high identity to vertebrate CPT1. This sea bream CPT1 mRNA is predominantly expressed in skeletal and cardiac muscle, with little expression in other tissues. Phylogenetic analysis of other vertebrate CPT1 sequences show that fish genomes contain a single gene related to mammalian CPT1B, and a further two multi-gene families related to mammalian CPT1A. Genes related to mammalian CPT1C are absent in fish. Therefore, based on both functional and evolutionary orthology to mammalian CPT1B, the sea bream CPT1 reported here is a CPT1B isoform. Sea bream CPT1B mRNA expression progressively decreases in heart and muscle up to 12h after last feeding, but returns to initial, non-fasted levels after 72h. In contrast, in liver non-fasted expression is low, but strongly increases at 24 and 72h after last feeding. In white muscle and liver, CPT1B mRNA expression is highly correlated with the expression of peroxisomal proliferator-activated receptor beta (PPARbeta). Thus fatty acid metabolism by CPT1B and its control by PPARs are similar in fish and mammals, but multiple genes for CPT1A-like proteins in fish also suggest different and more complex pathways of lipid utilisation than in mammals. PMID:20601065

  12. Lipid hydrolysis and oxidation in farmed gilt head sea bream (Sparus aurata) slaughtered and chilled under different icing conditions

    Energy Technology Data Exchange (ETDEWEB)

    Aubourg, S. P.; Alvarez, V.; Pena, J.

    2010-07-01

    This work focuses on the slaughtering and chilled storage (up to 14 days) related to the commercialization of fresh farmed gilt head sea bream (Spa rus aurata). A slurry ice (SI) system was applied and evaluated in comparison to traditional flake ice (FI). Lipid hydrolysis and oxidation were analyzed and compared to sensory acceptance and trimethylamine (TMA) formation. An important quality loss could be assessed in fish during slaughtering and chilling storage, according to sensory assessment and TMA formation. However, lipid damage development was found relatively low, in agreement to the different lipid quality indexes checked (lipid hydrolysis; primary, secondary and tertiary lipid oxidation). No development of rancid odor and no polyunsaturated fatty acid losses were detected. The employment of SI as a slaughtering and chilling strategy was found useful to inhibit quality loss in gilthead seabream resulting in a shelf life increase and a TMA and free fatty acid formation inhibition. (Author)

  13. Effect of short chain fructooligosaccharides (scFOS) on immunological status and gut microbiota of gilthead sea bream (Sparus aurata) reared at two temperatures.

    Science.gov (United States)

    Guerreiro, Inês; Serra, Cláudia R; Enes, Paula; Couto, Ana; Salvador, Andreia; Costas, Benjamín; Oliva-Teles, Aires

    2016-02-01

    The effects of dietary short chain fructooligosaccharides (scFOS) incorporation on hematology, fish immune status, gut microbiota composition, digestive enzymes activities, and gut morphology, was evaluated in gilthead sea bream (Sparus aurata) juveniles reared at 18 °C and 25 °C. For that purpose, fish with 32 g were fed diets including 0, 0.1, 0.25 and 0.5% scFOS during 8 weeks. Overall, scFOS had only minor effects on gilthead sea bream immune status. Lymphocytes decreased in fish fed the 0.1% scFOS diet. Fish fed the 0.5% scFOS diet presented increased nitric oxide (NO) production, while total immunoglobulins (Ig) dropped in those fish, but only in the ones reared at 25 °C. Red blood cells, hemoglobin, bactericidal activity and NO were higher at 25 °C, whereas total white blood cells, circulating thrombocytes, monocytes and neutrophils were higher at 18 °C. In fish fed scFOS, lymphocytes were higher at 18 °C. Total Ig were also higher at 18 °C but only in fish fed 0.1% and 0.5% scFOS diets. No differences in gut bacterial profiles were detected by PCR-DGGE (polymerase chain reaction denaturing gradient gel electrophoresis) between dietary treatments. However, group's similarity was higher at 25 °C. Digestive enzymes activities were higher at 25 °C but were unaffected by prebiotics incorporation. Gut morphology was also unaffected by dietary prebiotic incorporation. Overall, gut microbiota composition, digestive enzymes activities and immunity parameters were affected by rearing temperature whereas dietary scFOS incorporation had only minor effects on these parameters. In conclusion, at the tested levels scFOS does not seem worthy of including it in gilthead sea bream juveniles diets. PMID:26721230

  14. Impact of Fishmeal Replacement in Diets for Gilthead Sea Bream (Sparus aurata on the Gastrointestinal Microbiota Determined by Pyrosequencing the 16S rRNA Gene.

    Directory of Open Access Journals (Sweden)

    G Estruch

    Full Text Available Recent studies have demonstrated the impact of diet on microbiota composition, but the essential need for the optimization of production rates and costs forces farms and aquaculture production to carry out continuous dietary tests. In order to understand the effect of total fishmeal replacement by vegetable-based feed in the sea bream (Sparus aurata, the microbial composition of the stomach, foregut, midgut and hindgut was analysed using high-throughput 16S rDNA sequencing, also considering parameters of growth, survival and nutrient utilisation indices.A total of 91,539 16S rRNA filtered-sequences were analysed, with an average number of 3661.56 taxonomically assigned, high-quality sequences per sample. The dominant phyla throughout the whole gastrointestinal tract were Actinobacteria, Protebacteria and Firmicutes. A lower diversity in the stomach in comparison to the other intestinal sections was observed. The microbial composition of the Recirculating Aquaculture System was totally different to that of the sea bream gastrointestinal tract. Total fishmeal replacement had an important impact on microbial profiles but not on diversity. Streptococcus (p-value: 0.043 and Photobacterium (p-value: 0.025 were highly represented in fish fed with fishmeal and vegetable-meal diets, respectively. In the stomach samples with the vegetable diet, reads of chloroplasts and mitochondria from vegetable dietary ingredients were rather abundant. Principal Coordinate Analysis showed a clear differentiation between diets in the microbiota present in the gut, supporting the presence of specific bacterial consortia associated with the diet.Although differences in growth and nutritive parameters were not observed, a negative effect of the vegetable diet on the survival rate was determined. Further studies are required to shed more light on the relationship between the immune system and sea bream gastrointestinal tract microbiota and should consider the modulation of

  15. Impact of Fishmeal Replacement in Diets for Gilthead Sea Bream (Sparus aurata) on the Gastrointestinal Microbiota Determined by Pyrosequencing the 16S rRNA Gene.

    Science.gov (United States)

    Estruch, G; Collado, M C; Peñaranda, D S; Tomás Vidal, A; Jover Cerdá, M; Pérez Martínez, G; Martinez-Llorens, S

    2015-01-01

    Recent studies have demonstrated the impact of diet on microbiota composition, but the essential need for the optimization of production rates and costs forces farms and aquaculture production to carry out continuous dietary tests. In order to understand the effect of total fishmeal replacement by vegetable-based feed in the sea bream (Sparus aurata), the microbial composition of the stomach, foregut, midgut and hindgut was analysed using high-throughput 16S rDNA sequencing, also considering parameters of growth, survival and nutrient utilisation indices.A total of 91,539 16S rRNA filtered-sequences were analysed, with an average number of 3661.56 taxonomically assigned, high-quality sequences per sample. The dominant phyla throughout the whole gastrointestinal tract were Actinobacteria, Protebacteria and Firmicutes. A lower diversity in the stomach in comparison to the other intestinal sections was observed. The microbial composition of the Recirculating Aquaculture System was totally different to that of the sea bream gastrointestinal tract. Total fishmeal replacement had an important impact on microbial profiles but not on diversity. Streptococcus (p-value: 0.043) and Photobacterium (p-value: 0.025) were highly represented in fish fed with fishmeal and vegetable-meal diets, respectively. In the stomach samples with the vegetable diet, reads of chloroplasts and mitochondria from vegetable dietary ingredients were rather abundant. Principal Coordinate Analysis showed a clear differentiation between diets in the microbiota present in the gut, supporting the presence of specific bacterial consortia associated with the diet.Although differences in growth and nutritive parameters were not observed, a negative effect of the vegetable diet on the survival rate was determined. Further studies are required to shed more light on the relationship between the immune system and sea bream gastrointestinal tract microbiota and should consider the modulation of the microbiota to

  16. Molecular endocrine changes of Gh/Igf1 axis in gilthead sea bream (Sparus aurata L.) exposed to different environmental salinities during larvae to post-larvae stages.

    Science.gov (United States)

    Mohammed-Geba, Khaled; Yúfera, Manuel; Martínez-Rodríguez, Gonzalo; Mancera, Juan Miguel

    2016-08-01

    The influence of acclimation of the euryhaline gilthead sea bream (Sparus aurata) larvae/post-larvae to brackish water on growth, energetic contents, and mRNA levels of selected hormones and growth-regulating hypothalamic neurohormones was assessed. Specimens from 49 days post-hatching were acclimated during 28 days to two different environmental salinities: 38 and 20 psu (as brackish water). Both groups were then transferred to 38 psu and acclimated for an additional week. Early juveniles were sampled after 28 days of acclimation to both salinities and one week after transfer to 38 psu. Pituitary adenylate cyclase-activating peptide (adcyap1; pacap), somatostatin-I (sst1), growth hormone (gh1), insulin-like growth factor-I (igf1), and prolactin (prl) mRNA expression were all studied by QPCR. Post-larvae acclimated to 20 psu showed better growth performance and body energetic content than post-larvae maintained at 38 psu. prl, adcyap1, and igf1 mRNA expression levels increased in 20-psu-acclimated post-larvae but decreased upon transfer to 38 psu. GH1 expression did not show significant changes under both experimental conditions. Our results suggested an enhanced general performance for post-larvae in brackish water, supported by the actions of adcyap1, igf1, and prl. PMID:26947706

  17. Biomagnification of organochlorine pollutants in farmed and wild gilthead sea bream (Sparus aurata) and stable isotope characterization of the trophic chains

    International Nuclear Information System (INIS)

    Organochlorine pollutants (pesticides and polychlorinated biphenyls) were analysed in farmed and wild gilthead sea bream (Sparus aurata) tissues (white muscle and liver) from the Western Mediterranean (Spain) and in their diets. Determination was carried out by gas chromatography coupled to tandem mass spectrometry after clean up of the fatty extracts by normal phase HPLC, with detection limits around 0.1 ng/g. Carbon and nitrogen stable isotope ratios were also determined in the samples. Organochlorine compounds concentration was found to be uniform throughout the year in farmed fish, in both white muscle and liver. In contrast, wild fish showed contamination profiles that reflect environmental factors and the biological cycle. Although biomagnification factors for white muscle and liver were found to be 2.4 and 3.0, respectively for farmed fish, and 0.15 and 0.54 for wild specimens, wild fish presented higher levels of organochlorine contaminants than farmed fish. Nitrogen stable isotopes determination in muscle from wild and farmed sea bream during the year gave us a profile related to the biological cycle. δ15N mean values from farmed fish were 2.0 per mille higher than from wild fish throughout the year that corresponding to close to one trophic step. δ13C values were stable during the year, and also more enriched in the case of farmed fish. The low levels of contaminants found in the feed supplied to farmed fish explain the organochlorine concentrations in their tissues which remain below wild fish, in spite of the intensive culture conditions and higher trophic level of cultured specimens

  18. Immunocytochemical and ultrastructural characterization of mammosomatotrope-, growth hormone-, and prolactin-cells from the gilthead sea bream (Sparus aurata l., Teleostei): an ontogenic study.

    Science.gov (United States)

    Villaplana, Mariano; García Ayala, Alfonsa; García Hernández, Maria Pilar; Agulleiro, Blanca

    2003-03-01

    Growth hormone (GH), prolactin (PRL), and mammosomatotrope (MS) cells of gilthead sea bream, Sparus aurata, a teleost fish, were studied in specimens from hatching to 15 months (adults) using conventional electron microscopy and an immunogold method using anti-tilapia GH sera and anti-chum salmon PRL serum. MS cells, immunoreactive to both anti-GH sera and anti-PRL sera, had been first identified in fish in a previous study in newly hatched larvae and in older larvae and juvenile specimens of Sparus aurata by light microscopic immunocytochemistry. In the present work, MS cells reacted positively to immunogold label only in older larvae and juveniles and their secretory granules immunoreacted with both GH and PRL antisera or with only one of them. MS cells were ultrastructurally similar to the PRL cells, with which they coincided in time. This is the first report on the ultrastructural characterization of MS cells in fish. In adults, the secretory granules of GH cells (immunoreactive to anti-GH serum) were mainly round, of variable size, and had a homogeneous, highly electron-dense content. Irregularly shaped secretory granules were also present. PRL cells (immunoreactive to anti-PRL serum) were usually observed in a follicular arrangement; they showed few, small, and mainly round secretory granules with a homogeneous and high or medium electron-dense content. Some oval or elongated secretory granules were also observed. GH and PRL cells that showed involutive features were also found. In newly hatched larvae, GH, PRL, and MS cells could not be distinguished either by their ultrastructure or by the immunogold labeling of the secretory granules. In 1-day-old larvae, presumptive GH and PRL cells were observed according to their position in the pituitary gland. In 2-day-old larvae, a few cells showed some of the ultrastructural features described for GH and PRL cells of adults. During development, the number, size, and shape of the secretory granules in both cell types

  19. Vessel noise pollution as a human threat to fish: assessment of the stress response in gilthead sea bream (Sparus aurata, Linnaeus 1758).

    Science.gov (United States)

    Celi, Monica; Filiciotto, Francesco; Maricchiolo, Giulia; Genovese, Lucrezia; Quinci, Enza Maria; Maccarrone, Vincenzo; Mazzola, Salvatore; Vazzana, Mirella; Buscaino, Giuseppa

    2016-04-01

    This study examined the effects of boat noise pollution on the stress indices of gilthead sea bream (Sparus aurata, Linnaeus 1758). To assess the stress response in these fish, biometric values and plasma parameters such as ACTH, cortisol, glucose, lactate, haematocrit, Hsp70, total protein, cholesterol, triglycerides and osmolarity were analysed. After acclimatization of the animals, the experiment was carried out in a tank fitted with underwater speakers where the fish were exposed to sound treatments (in duplicate) consisting of: 10 days of no sound (control treatment; the animals were only exposed to the experimental tank's background noise) and 10 days of noise derived from original recordings of motor boats, including recreational boats, hydrofoil, fishing boat and ferry boat (vessel noise treatment). The exposure to noise produced significant variations in almost all the plasma parameters assessed, but no differences were observed in weights and fork lengths. A PERMANOVA analysis highlighted significantly increased values (p < 0.05) of ACTH, cortisol, glucose, lactate, haematocrit, Hsp70, cholesterol, triglycerides and osmolarity in the fish exposed to vessel noise for 10 days. This study clearly highlights that anthropogenic noise negatively affects fish, and they are valuable targets for detailed investigations into the effects of this global pollutant. Finally, these experimental studies could represent part of the science that is able to improve the quality of the policies related to management plans for maritime spaces (Marine Strategy Framework Directive 56/2008 CE) that are aimed at stemming this pollutant phenomenon. PMID:26581747

  20. Multidisciplinary analytical investigation of phospholipids and triglycerides in offshore farmed gilthead sea bream (Sparus aurata) fed commercial diets.

    Science.gov (United States)

    Anedda, Roberto; Piga, Carlo; Santercole, Viviana; Spada, Simona; Bonaglini, Elia; Cappuccinelli, Roberto; Mulas, Gilberto; Roggio, Tonina; Uzzau, Sergio

    2013-06-01

    In this work, a quantitative characterisation of lipid (both triglycerides and phospholipids) rearrangements in the muscle of offshore-raised gilthead sea bream was carried out as a function of fish growth between April and September. Relative percentages of lipid classes and fatty acids/acyls composition of the commercial feeds and fish dorsal muscles were assessed by means of an interdisciplinary analytical approach. A combination of preparative chemistry and experimental results from NMR spectroscopy, GC, 3D-TLC as well as proximate analysis permitted the observed growth parameters in key metabolic events to be linked with fish fattening and lipid turnover. While defined effects of feed composition on fatty acid profiles of fillets were ascertained, the relative increase of fatty acyls in triglycerides and phospholipids were also estimated enabling detailed evaluation of TAG:PL ratio in adult offshore-farmed gilthead sea bream. NMR was also used to quantify PUFA regiospecific distribution in TAG and PL. PMID:23411224

  1. UTILIZATION OF CORN GLUTEN MEAL AS A PROTEIN SOURCE IN DIETS FOR GILTHEAD SEA BREAM (Sparus aurata L. JUVENILES

    Directory of Open Access Journals (Sweden)

    Murat Yiğit

    2012-01-01

    Full Text Available The utilization of corn gluten meal (CGM was evaluated as a partial fish meal (FM substitute in practical diets for gilthead sea bream juveniles. Four test diets (isonitrogenous and isoca¬loric, 52% protein and 10% lipid, 19 kJ/g diet containing increasing levels of CGM were for¬mulated to replace anchovy meal at levels of 0%, 10%, 20%, and 30%. Triplicate groups of ju¬venile sea bream (initial body weight of 1.5 g were reared in a Recirculating Aquaculture System (RAS over 45 days at 18±2°C. Fish fed a diet containing 10% of CGM showed com¬parable growth performance similar to the control diet containing FM as the sole protein source. No mortality was observed in all treatment groups. Dietary CGM inclusion levels of 20% and 30% showed lower growth performance, feed utilization, and protein efficiency com¬pared to the control and the 10% CGM inclusion diets. However these values were not signifi¬cantly different among fish fed the CGM10 and CGM20 diets. Economical analyses also con¬firmed the growth related experimental results in terms of best profit obtained with the 10% CGM inclusion diet. Results in the present study showed that CGM alone without any amino acid supplements can substitute FM up to 10% with no adverse effects on growth performance, feed utilization, or economical inputs in gilthead sea bream juveniles.

  2. Necesidades de mantenimiento y crecimiento de la Dorada (Sparus aurata L.)

    OpenAIRE

    Jauralde García, Ignacio

    2016-01-01

    [EN] The gilthead sea bream (Sparus aurata) is the fish specie more produced in the Mediterranean countries, however, the real nutrient requirements have not been studied until recently. The aim of the present thesis was to develop a multi-factorial model that allow to estimate the nutritional requirements of the gilthead sea bream, considering several growth situations for the weight range between 20 and 450g, as well as to design diets with a optimum nutritive composition....

  3. Dietary Lipid and Carbohydrate Interactions: Implications on Lipid and Glucose Absorption, Transport in Gilthead Sea Bream (Sparus aurata) Juveniles.

    Science.gov (United States)

    Castro, Carolina; Corraze, Geneviève; Basto, Ana; Larroquet, Laurence; Panserat, Stéphane; Oliva-Teles, Aires

    2016-06-01

    A digestibility trial was performed with gilthead sea bream juveniles (IBW = 72 g) fed four diets differing in lipid source (fish oil, FO; or a blend of vegetable oil, VO) and starch content (0 %, CH-; or 20 %, CH+) to evaluate the potential interactive effects between carbohydrates and VO on the processes involved in digestion, absorption and transport of lipids and glucose. In fish fed VO diets a decrease in lipid digestibility and in cholesterol (C), High Density Lipoprotein(HDL)-C and Low Density Lipoprotein (LDL)-C (only in CH+ group) were recorded. Contrarily, dietary starch induced postprandial hyperglycemia and time related alterations on serum triacylglycerol (TAG), phospholipid (PL) and C concentrations. Fish fed a CH+ diet presented lower serum TAG than CH- group at 6 h post-feeding, and the reverse was observed at 12 h post-feeding for TAG and PL. Lower serum C and PL at 6 h post-feeding were recorded only in VOCH+ group. No differences between groups were observed in hepatic and intestinal transcript levels of proteins involved in lipid transport and hydrolysis (FABP, DGAT, GPAT, MTP, LPL, LCAT). Lower transcript levels of proteins related to lipid transport (ApoB, ApoA1, FABP2) were observed in the intestine of fish fed the CH+ diet, but remained unchanged in the liver. Overall, transcriptional mechanisms involved in lipid transport and absorption were not linked to changes in lipid serum and digestibility. Dietary starch affected lipid absorption and transport, probably due to a delay in lipid absorption. This study suggests that a combination of dietary VO and starch may negatively affect cholesterol absorption and transport. PMID:27023202

  4. Intraspecific variation in aerobic and anaerobic locomotion: gilthead sea bream (Sparus aurata) and Trinidadian guppy (Poecilia reticulata) do not exhibit a trade-off between maximum sustained swimming speed and minimum cost of transport.

    Science.gov (United States)

    Svendsen, Jon C; Tirsgaard, Bjørn; Cordero, Gerardo A; Steffensen, John F

    2015-01-01

    Intraspecific variation and trade-off in aerobic and anaerobic traits remain poorly understood in aquatic locomotion. Using gilthead sea bream (Sparus aurata) and Trinidadian guppy (Poecilia reticulata), both axial swimmers, this study tested four hypotheses: (1) gait transition from steady to unsteady (i.e., burst-assisted) swimming is associated with anaerobic metabolism evidenced as excess post exercise oxygen consumption (EPOC); (2) variation in swimming performance (critical swimming speed; U crit) correlates with metabolic scope (MS) or anaerobic capacity (i.e., maximum EPOC); (3) there is a trade-off between maximum sustained swimming speed (U sus) and minimum cost of transport (COTmin); and (4) variation in U sus correlates positively with optimum swimming speed (U opt; i.e., the speed that minimizes energy expenditure per unit of distance traveled). Data collection involved swimming respirometry and video analysis. Results showed that anaerobic swimming costs (i.e., EPOC) increase linearly with the number of bursts in S. aurata, with each burst corresponding to 0.53 mg O2 kg(-1). Data are consistent with a previous study on striped surfperch (Embiotoca lateralis), a labriform swimmer, suggesting that the metabolic cost of burst swimming is similar across various types of locomotion. There was no correlation between U crit and MS or anaerobic capacity in S. aurata indicating that other factors, including morphological or biomechanical traits, influenced U crit. We found no evidence of a trade-off between U sus and COTmin. In fact, data revealed significant negative correlations between U sus and COTmin, suggesting that individuals with high U sus also exhibit low COTmin. Finally, there were positive correlations between U sus and U opt. Our study demonstrates the energetic importance of anaerobic metabolism during unsteady swimming, and provides intraspecific evidence that superior maximum sustained swimming speed is associated with superior swimming

  5. Neuroglial cells in long-term primary cultures from the gilthead sea bream (Sparus aurata L.: new functional in vitro model from bony fish brain

    Directory of Open Access Journals (Sweden)

    Gerardo Centoducati

    2013-01-01

    Full Text Available Neuroglia has been historically considered the “glue” of the nervous system, as the ancient Greek name suggests, being simply referred as non-neuronal cells, with supporting functions for neurons in the CNS of mammalian and lower vertebrates. All around the world, approximately 283 cell lines were obtained from fish, yet none of these was from the brain of Sparus aurata, neither in cell lines nor as primary culture. Here we describe a novel in vitro reproducible neuroglial marine model for establishing primary neuroglial cell cultures, by dissociating the whole brain of seabream juveniles. We showed that proliferating neural stem cells produced alongside three generating lineages, such as neuronal precursor cells, astroglial precursor cells and oligodendroglia precursor cells, which developed respectively neurons, astrocytes and oligodendrocytes. The radial glia, finely described by morphological studies and immunochemical antigen expression, showed a peculiar spatial distribution, giving rise simultaneously both to astrocytes and neuronal precursors within a highly proliferative assemblate. Radial glia cells were assessed by glial fibrillary acidic protein (GFAP and vimentin reactivity, astrocytes by GFAP, neurons by the neuron-specific markers for ubiquitin carboxy-terminal hydrolase 1 (UCHL1 and intermediate filament associated protein (NF, whereas myelinating oligodendrocytes were immunostained with anti-myelin basic protein (MBP and anti-O4. Our findings suggest that seabream neuroglial cells gain in 3-4 weeks of culturing proliferation, neuroglial differentiation, and oligodendrocyte maturation with myelination, thus disclosing on the possibility that mixed neuroglial cultures can accelerate the maturation of oligodendrocytes and the regeneration of CNS injury in fish.

  6. Sea Cucumber Meal as Alternative Protein Source to Fishmeal in Gilthead Sea Bream (Sparus aurata) Nutrition: Effects on Growth and Welfare

    OpenAIRE

    Piccinno, Manuela; Schiavone, Roberta; Zilli, Loredana; Sicuro, Benedetto; Storelli, Carlo; Vilella, Sebastiano

    2013-01-01

    The aim of the present study was to evaluate the effect of sea cucumber meal on gilthead sea bream growth. Two diets were used: a fishmeal based diet (control) and a diet containing 18% of sea cucumber meal inclusion (HM). A 100-days growth trial was carried out (120 fish, initial mean body weight of 35.28 ± 9.31 g). The experimental plan used was monofactorial, balanced with three replicates for two experimental treatments (fish diet). The diets were isolipidic (CL 15.80 ± 0.5 %), isonit...

  7. Effect of high-level fish meal replacement by plant proteins in gilthead sea bream (Sparus aurata) on growth and body/fillet quality traits

    OpenAIRE

    M. de Francesco; Parisi, G.; Perez Sanchez, J.; Gomez Requeni, P; Medale, Francoise; Kaushik, Sadasivam; M. Mecatti; Poli, B

    2007-01-01

    Juvenile gilthead sea bream (initial body weight ca. 100 g) were reared in an indoor flow through marine water system for 1 year. Fish were fed two isoenergetic [19.2 kJ g−1 dry matter (DM)] and isoproteic (426 g kg−1 DM) diets either based on fish meal (diet FM) or on a mixture of plant protein sources (diet PP), replacing 75% of fish meal protein. The growth trial was conducted in duplicate, two tanks for each dietary treatment. Growth performance and feed utilization were regis...

  8. Plant oils' inclusion in high fish meal-substituted diets: Effect on digestion and nutrient absorption in gilthead sea bream (Sparus aurata L.)

    OpenAIRE

    Santigosa, Ester; García-Meilán, Irene; Valentín, Juana María; Navarro, Isabel; Pérez-Sánchez, Jaume; Gallardo, María Ángeles

    2011-01-01

    Here, we performed an 11-week trial to study the effects of four experimental diets on the digestion, nutrient absorption and intestinal histology of gilthead sea bream. The diets were formulated with a low fish meal content (25%) and were rich (75%) in plant proteins. Fish oil (FO) was replaced at 0%, 33%, 66% and 100% by graded levels of a blend of vegetable oils (VO) (diets FO, 33VO, 66VO and 100VO respectively). Protease activity increased in the pyloric caeca (PC) and decreased in the pr...

  9. IGF-I and IGF-II effects on local IGF system and signaling pathways in gilthead sea bream (Sparus aurata) cultured myocytes.

    Science.gov (United States)

    Azizi, Sheida; Nematollahi, Mohammad Ali; Mojazi Amiri, Bagher; Vélez, Emilio J; Salmerón, Cristina; Chan, Shu Jin; Navarro, Isabel; Capilla, Encarnación; Gutiérrez, Joaquim

    2016-06-01

    The insulin-like growth factors (IGFs) have a fundamental role in a vast range of functions acting through a tyrosine-kinase receptor (IGF-IR). IGFs in muscle can affect the expression of components of the local IGF system, myogenic regulatory factors (MRFs), proliferating (proliferating cell nuclear antigen, PCNA) or differentiating molecules (myosin heavy chain, MHC) and, lead to the activation of different signaling pathways. The response of all these genes to IGFs incubation at two different times in day 4 cultured myocytes of gilthead sea bream was analyzed. Both IGFs increased the expression of IGF-I and IGFBP-5, but showed different effects on the receptors, with IGF-I suppressing the expression of both isoforms (IGF-IRa and IGF-IRb) and IGF-II up-regulating only IGF-IRb. Moreover, the protein levels of PCNA and target of rapamycin (TOR) increased after IGF-II incubation, although a decline in Myf5 and a rise in MHC gene expression was caused by IGF-I. Taken together, these results provide evidence for the importance of IGFs on controlling muscle development and growth in gilthead sea bream and suggest that each IGF may be preferentially acting through a specific IGF-IR. Moreover, the data support the hypothesis that IGF-II has a more important role during proliferation, whereas IGF-I seems to be relevant for the differentiation phase of myogenesis. PMID:26602376

  10. Effect of ration size on fillet fatty acid composition, phospholipid allostasis and mRNA expression patterns of lipid regulatory genes in gilthead sea bream (Sparus aurata).

    Science.gov (United States)

    Benedito-Palos, Laura; Calduch-Giner, Josep A; Ballester-Lozano, Gabriel F; Pérez-Sánchez, Jaume

    2013-04-14

    The effect of ration size on muscle fatty acid (FA) composition and mRNA expression levels of key regulatory enzymes of lipid and lipoprotein metabolism have been addressed in juveniles of gilthead sea bream fed a practical diet over the course of an 11-week trial. The experimental setup included three feeding levels: (i) full ration until visual satiety, (ii) 70 % of satiation and (iii) 70 % of satiation with the last 2 weeks at the maintenance ration. Feed restriction reduced lipid content of whole body by 30 % and that of fillet by 50 %. In this scenario, the FA composition of fillet TAG was not altered by ration size, whereas that of phospholipids was largely modified with a higher retention of arachidonic acid and DHA. The mRNA transcript levels of lysophosphatidylcholine acyltransferases, phosphatidylethanolamine N-methyltransferase and FA desaturase 2 were not regulated by ration size in the present experimental model. In contrast, mRNA levels of stearoyl-CoA desaturases were markedly down-regulated by feed restriction. An opposite trend was found for a muscle-specific lipoprotein lipase, which is exclusive of fish lineage. Several upstream regulatory transcriptions were also assessed, although nutritionally mediated changes in mRNA transcripts were almost reduced to PPARα and β, which might act in a counter-regulatory way on lipolysis and lipogenic pathways. This gene expression pattern contributes to the construction of a panel of biomarkers to direct marine fish production towards muscle lean phenotypes with increased retentions of long-chain PUFA. PMID:22856503

  11. Trace elements in farmed and wild gilthead seabream, Sparus aurata

    International Nuclear Information System (INIS)

    In the present study, we sought to determine the concentration of several trace elements (total and organic mercury, selenium, cadmium, lead, arsenic, copper, chromium, iron, manganese, molybdenum, vanadium and zinc) in muscle tissue of gilthead seabream (Sparus aurata), an edible finfish species that is widely cultured in the Mediterranean area. The phenomenon of bioaccumulation and bioamplification (especially of mercury) was investigated by comparing farmed fish with wild specimens of the same species. Farmed specimens showed mercury and arsenic concentrations significantly lower than wild specimens, and no relationship was found between mercury concentration and body size. The lower level of methylmercury in farmed fish has important implications for consumer health.

  12. Toxic Effects of Domoic Acid in the Seabream Sparus aurata

    Science.gov (United States)

    Nogueira, Isabel; Lobo-da-Cunha, Alexandre; Afonso, António; Rivera, Socorro; Azevedo, Joana; Monteiro, Rogério; Cervantes, Rosa; Gago-Martinez, Ana; Vasconcelos, Vítor

    2010-01-01

    Neurotoxicity induced in fish by domoic acid (DA) was assessed with respect to occurrence of neurotoxic signs, lethality, and histopathology by light microscopy. Sparus aurata were exposed to a single dose of DA by intraperitoneal (i.p.) injection of 0, 0.45, 0.9, and 9.0 mg DA kg−1 bw. Mortality (66.67 ± 16.67%) was only observed in dose of 9.0 mg kg−1 bw. Signs of neurological toxicity were detected for the doses of 0.9 and 9.0 mg DA kg−1 bw. Furthermore, the mean concentrations (±SD) of DA detected by HPLC-UV in extracts of brain after exposure to 9.0 mg DA kg−1 bw were 0.61 ± 0.01, 0.96 ± 0.00, and 0.36 ± 0.01 mg DA kg−1 tissue at 1, 2, and 4 hours. The lack of major permanent brain damage in S. aurata, and reversibility of neurotoxic signs, suggest that lower susceptibility to DA or neuronal recovery occurs in affected individuals. PMID:21116416

  13. Retinal development in the gilthead seabream Sparus aurata.

    Science.gov (United States)

    Pavón-Muñoz, T; Bejarano-Escobar, R; Blasco, M; Martín-Partido, G; Francisco-Morcillo, J

    2016-02-01

    The retinal development of the gilthead seabream Sparus aurata has been analysed from late embryonic development to juvenile stages using classical histological and immunohistological methods. Five significant phases were established. Phases 1 and 2 comprise the late embryonic and hatching stages, respectively. The results indicate that during these early stages the retina is composed of a single neuroblastic layer that consists of undifferentiated retinal progenitor cells. Phase 3 (late prolarval stage) is characterized by the emergence of the retinal layers and the appearance of neurochemical profiles in differentiating photoreceptors, amacrine and ganglion cells. Phases 4 and 5 comprise the late larval and juvenile stages. In these stages, all the retinal cell types can be detected immunohistochemically. All the maturational events described are first detected in the central retina and, as development progresses, spread to the rest of the retina following a central-to-peripheral gradient. The results of this study suggest that S. aurata is an altricial teleost species that hatches with a morphologically undifferentiated retina. The most relevant processes involved in retinogenesis occur during the late prolarval stage (phase 3). PMID:26507100

  14. Toxic effects of domoic acid in the seabream Sparus aurata.

    Science.gov (United States)

    Nogueira, Isabel; Lobo-da-Cunha, Alexandre; Afonso, António; Rivera, Socorro; Azevedo, Joana; Monteiro, Rogério; Cervantes, Rosa; Gago-Martinez, Ana; Vasconcelos, Vítor

    2010-01-01

    Neurotoxicity induced in fish by domoic acid (DA) was assessed with respect to occurrence of neurotoxic signs, lethality, and histopathology by light microscopy. Sparus aurata were exposed to a single dose of DA by intraperitoneal (i.p.) injection of 0, 0.45, 0.9, and 9.0 mg DA kg(-1) bw. Mortality (66.67 ± 16.67%) was only observed in dose of 9.0 mg kg(-1) bw. Signs of neurological toxicity were detected for the doses of 0.9 and 9.0 mg DA kg(-1) bw. Furthermore, the mean concentrations (±SD) of DA detected by HPLC-UV in extracts of brain after exposure to 9.0 mg DA kg(-1) bw were 0.61 ± 0.01, 0.96 ± 0.00, and 0.36 ± 0.01 mg DA kg(-1) tissue at 1, 2, and 4 hours. The lack of major permanent brain damage in S. aurata, and reversibility of neurotoxic signs, suggest that lower susceptibility to DA or neuronal recovery occurs in affected individuals. PMID:21116416

  15. Determination of sulfadiazine, trimethoprim, and N(4) -acetyl-sulfadiazine in fish muscle plus skin by Liquid Chromatography-Mass Spectrometry. Withdrawal-time calculation after in-feed administration in gilthead sea bream (Sparus aurata L.) fed two different diets.

    Science.gov (United States)

    Zonaras, V; Tyrpenou, A; Alexis, M; Koupparis, M

    2016-10-01

    This study presents a depletion study for sulfadiazine and trimethoprim in muscle plus skin of gilthead sea bream (Sparus aurata L.). N(4) -acetyl-sulfadiazine, the main metabolite of sulfadiazine (SDZ), was also examined. The fish were held in seawater at a temperature of 24-26 °C. SDZ and trimethoprim (TMP) were administered orally with medicated feed for five consecutive days at daily doses of 25 mg SDZ and 5 mg TMP per kg of fish body weight per day. Two different diets, fish oil- and plant oil-based diets, were investigated. Ten fish were sampled at each of the days 1, 3, 5, 6, 8, 9, 10, and 12 after the start of veterinary medicine administration. However for the calculation of the withdrawal periods, sampling day 1 was set as 24 h after the last dose of the treatment. Fish samples were analyzed for SDZ, TMP, and acetyl-sulfadiazine (AcSDZ) residues by liquid chromatography-mass spectrometry. SDZ and TMP concentrations declined rapidly from muscle plus skin. Considering a maximum residue limit of 100 μg/kg for the total of sulfonamides and 50 μg/kg for TMP residues in fish muscle plus skin, the withdrawal periods of the premix trimethoprim-sulfadiazine 50% were calculated as 5 and 6 days, at 24-26 °C, in fish oil (FO) and plant oil (PO) groups, respectively. The investigation of this work is important to protect consumers by controlling the undesirable residues in fish. PMID:26987772

  16. The effects of swimming pattern on the energy use of gilthead seabream (Sparus aurata L.)

    DEFF Research Database (Denmark)

    Steinhausen, Maria Faldborg; Steffensen, John Fleng; Andersen, Niels Gerner

    2010-01-01

    Oxygen consumption ( ) was measured for gilthead seabream (Sparus aurata) during spontaneous and forced activities. During spontaneous activity, the swimming pattern was analysed for the effect on   on the average speed (U), turning rate (¿) and change in speed (¿U). All swimming characteristics ...

  17. Ontogenia da cartilagem e do osso em dourada, Sparus aurata (Linnaeus, 1758)

    OpenAIRE

    Faustino, Manuel Almeida dos Ramos

    1996-01-01

    Dissertação de mestrado, Química Celular, Unidade de Ciências Exactas e Humanas, Universidade do Algarve, 1996 A dourada (Sparus aurata L) é uma das mais importantes espécies comerciais do sul da Europa, tendo o seu cultivo em cativeiro sido grandemente incrementado, entre nós, na última década. O estudo e caracterização da ontogenia das estruturas que formam o esqueleto, fornece-nos conhecimentos essenciais, para posteriores melhoramentos na produção de larvas e, provavelmente um melho...

  18. Envasado, conservación y desarrollo de nuevos productos de dorada (Sparus aurata)

    OpenAIRE

    Espinosa Vicente, Míriam del Carmen

    2015-01-01

    La presente Tesis Doctoral está constituida en cinco capítulos, orientados al estudio de estrategias que contribuyen a diversificar la oferta del sector acuícola a través de procesos de conservación y desarrollo de nuevos productos a partir de filetes de dorada. El objetivo general de la presente Tesis Doctoral fue la aplicación de estrategias y tecnologías en la conservación para incrementar la vida útil de filetes de dorada (Sparus aurata) frescos y cocinados. Para llevar a cabo este o...

  19. Metabolomics as a Powerful Tool for Molecular Quality Assessment of the Fish Sparus aurata

    Directory of Open Access Journals (Sweden)

    Francesco Capozzi

    2011-02-01

    Full Text Available The molecular profiles of perchloric acid solutions extracted from the flesh of Sparus aurata fish specimens, produced according to different aquaculture systems, have been investigated. The 1H-NMR spectra of aqueous extracts are indicative of differences in the metabolite content of fish reared under different conditions that are already distinguishable at their capture, and substantially maintain the same differences in their molecular profiles after sixteen days of storage under ice. The fish metabolic profiles are studied by top-down chemometric analysis. The results of this exploratory investigation show that the fish metabolome accurately reflects the rearing conditions. The level of many metabolites co-vary with the rearing conditions and a few metabolites are quantified including glycogen (stress indicator, histidine, alanine and glycine which all display significant changes dependent on the aquaculture system and on the storage times.

  20. Impact of synthetic surfactants and oil dispersants on the gills of juvenile gilthead (Sparus aurata L.

    Directory of Open Access Journals (Sweden)

    Maria Angela Masini

    2014-12-01

    Full Text Available Histological, scanning electron microscopic and immunohistochemical studies were performed on the gills of juvenile gilthead (Sparus aurata L. exposed to various concentrations of three anionic detergents: sodium dodecyl sulphate (SDS, safety sea cleaner 2 (SSC2, and FINASOL OSR2, in short term (96 h and chronic (28 days experiments. The mortality rate, median lethal concentration (LC50 and the no observed effect concentration (NOEC, were calculated. At concentrations above 10 mg/L, mortality was from 50 to 100% after exposure to SDS and SSC2, both in short term and chronic experiments. Gills were spongiotic and there were many lamellar fusions, membrane disruptions, hydropic degenerations and exfoliations of lamellar and interlamellar epithelium after 96 h of treatment and more markedly after chronic experiment. Microridges of the cell surface were less evident or absent. The enzymes of ion transport were down regulated. Exposure to FINASOL OSR2 (100 mg/L showed no evident branchial alteration.

  1. Cardicola aurata sp. n. (Digenea: Sanguinicolidae) from Mediterranean Sparus aurata L. (Teleostei: Sparidae) and its unexpected phylogenetic relationship with Paradeontacylix McIntosh, 1934.

    Science.gov (United States)

    Holzer, A S; Montero, F E; Repullés, A; Nolan, M J; Sitja-Bobadilla, A; Alvarez-Pellitero, P; Zarza, C; Raga, J A

    2008-12-01

    A new sanguinicolid trematode, Cardicola aurata sp. n., is described from gilthead seabream Sparus aurata L., from off the Spanish Mediterranean coast. The morphology of C. aurata sp. n. generally agrees with the diagnosis of the genus, however, in contrast to all other reported Cardicola spp. the male pore is located sub-medially at the posterior end of the body instead of sinistrally before the posterior end of the body. Based on a comparison of the morphology as well as partial 28S and ITS2 rDNA sequence data from the present species with that from closely related species, it was decided to emend the diagnosis of Cardicola rather than create a new genus, as the aberrant position of the male pore is likely to be an autapomorphy. The phylogenetic analyses revealed a close relationship between Cardicola and Paradeontacylix, two genera with considerable morphological differences; C. aurata sp. n. occupies a position intermediate to these genera. Thus, a morphological comparison of Cardicola, Paradeontacylix and Braya, a genus which is morphologically similar to Cardicola but clusters basal to the Cardicola/Paradeontacylix clade, was conducted. The results of this comparison showed that despite large differences with regard to body shape, the organisation of the internal organs is very similar in species of Cardicola and Paradeontacylix. The synopsis of morphological data and molecular phylogeny allows for interpretations regarding the importance of different morphological features for the phylogenetic inference of the Sanguinicolidae. PMID:18639649

  2. Quantitative trait loci for a neurocranium deformity, lack of operculum, in gilthead seabream (Sparus aurata L.).

    Science.gov (United States)

    Negrín-Báez, D; Navarro, A; Afonso, J M; Toro, M A; Zamorano, M J

    2016-04-01

    Lack of operculum, a neurocranial deformity, is the most common external abnormality to be found among industrially produced gilthead seabream (Sparus aurata L.), and this entails significant financial losses. This study conducts, for the first time in this species, a quantitative trait loci (QTL) analysis of the lack of operculum. A total of 142 individuals from a paternal half-sibling family (six full-sibling families) were selected for QTL mapping. They had previously shown a highly significant association with the prevalence of lack of operculum in a segregation analysis. All the fish were genotyped for 106 microsatellite markers using a set of multiplex PCRs (ReMsa1-ReMsa13). A linear regression methodology was used for the QTL analysis. Four QTL were detected for this deformity, two of which (QTLOP1 and QTLOP2) were significant. They were located at LG (linkage group) nine and LG10 respectively. Both QTL showed a large effect (about 27%), and furthermore, the association between lack of operculum and sire allelic segregation observed was statistically significant in the QTLOP1 analysis. These results represent a significant step towards including marker-assisted selection for this deformity in genetic breeding programmes to reduce the incidence of the deformity in the species. PMID:26995565

  3. Stress and Humoral Innate Immune Response of Gilthead Seabream Sparus aurata Cultured in Sea Cages.

    Science.gov (United States)

    Salati, Fulvio; Roncarati, Alessandra; Angelucci, Giulia; Fenza, Alessandra; Meluzzi, Adele

    2016-09-01

    Innate and acquired immune responses of Gilthead Seabream Sparus aurata was studied under normal culture and short-term stressful conditions for 18 months in offshore sea cages in Alghero Bay, Italy. Every 45 d, 50 fish were sampled and divided into two groups: fish in the first group (normal culture conditions) were bled after harvesting; fish in the second group were put into a tank under stressful conditions (crowding and confinement) and bled after 2 h. Innate humoral immunity, such as complement-like, hemagglutination, and lysozyme activities, was determined in the sera of both groups. Pathogen challenge was not performed, but the specific humoral immune response was assessed against the most common pathogens affecting cultured fish in Sardinia. Stressed fish, compared with the control, showed a lower lysozyme activity against Vibrio (Listonella) anguillarum, which was not clearly correlated with temperatures. Complement-like activity differed between the first and second half of the study and, at the end of the trial, a slightly higher activity was recorded in the controls than in the stressed fish. Hemagglutination activity was mainly higher in the stressed fish than in control fish. Confinement, crowding, and cold water temperature caused decreased lysozyme activity in short-term stressed Gilthead Seabream compared with those reared normally. The specific humoral immune response, against V. anguillarum, Tenacibaculum mesophilum, Enterococcus Seriolicida, and Aeromonas sobria, fluctuated during the rearing period, particularly during the first year of culture. Received October 12, 2015; accepted March 24, 2016. PMID:27485027

  4. Patterns of primary growth increments in otoliths of Sparus aurata larvae in relation to water temperature and food consumption

    OpenAIRE

    Morales-nin, B; Gutiérrez Merino, Emilia; Massutí, Sofia

    1995-01-01

    Sparus aurata larvae reared under controlled water-temperature conditions during the first 24 days after hatching displayed a linear relationship between age (t) and standard length (SL): SL = 2.68 + 0.19 t (r2 = 0.91l). Increments were laid down in the sagittae with daily periodicity starting on day of hatching. Standard length (SL) and sagittae radius (OR) were correlated: SL(mm) = 2.65 + 0.012 OR(mm). The series of measurements of daily growth increment widths (DWI), food density and water...

  5. EFFECTS OF FISH OIL, DHA OIL AND LECITHIN IN MICROPARTICULATE DIETS ON STRESS TOLERANCE OF LARVAL GILTHEAD SEABREAM (SPARUS AURATA)

    Institute of Scientific and Technical Information of China (English)

    刘镜恪; 王文琪; 李岿然; 雷霁霖

    2002-01-01

    The effects of natural fish oil, DHA oil and soybean lecithin in microparticulate diets on stress tolerance of larval gilthead seabream (Sparus aurata) were investigated after 15 days feeding trials. The tolerance of larval gilthead seabream to various stress factors such as exposure to air ( lack of dissolved oxygen), changes in water temperature (low) and salinity ( high) were determined. This study showed that microparticulate diet with natural fish oil and soybean lecithin was the most effective for increasing the tolerance of larval gilthead seabream to various stresses, and that microparticulate diet with natural fish oil and palmitic acid (16:0) was more effective than microparticulate diet with DHA oil and soybean lecithin.

  6. Immunotoxicological effects of inorganic arsenic on gilthead seabream (Sparus aurata L.)

    International Nuclear Information System (INIS)

    Highlights: •Short exposure to arsenic increases the hepato-somatic index and produces histopathological alterations in the liver. •Arsenic is bioaccumulated in the liver of gilthead seabream but no in the muscle. •Arsenic-exposure affects the innate immune system in the gilthead seabream. •Ten days of exposure to As enhances the immune parameters. -- Abstract: Arsenic (As) has been associated with multitude of animal and human health problems; however, its impact on host immune system has not been extensively investigated. In fish, there are very few works on the potential risks or problems associated to the presence of arsenic. In the present study we have evaluated the effects of exposure (30 days) to sub-lethal concentrations of arsenic (5 μM As2O3) in the teleost fish gilthead seabream (Sparus aurata), with special emphasis in the innate immune response. The arsenic concentration was determined using atomic fluorescence spectrometry (AFS) in liver and muscle of exposed fish showing As accumulation in the liver after 30 days of exposure. The hepatosomatic index was increased at significant extent after 10 days but returned to control values after 30 days of exposure. Histological alterations in the liver were observed including hypertrophy, vacuolization and cell-death processes. Focusing on the immunological response, the humoral immune parameters (seric IgM, complement and peroxidase activities) were no affected to a statistically significant extent. Regarding the cellular innate parameters, head-kidney leucocyte peroxidase, respiratory burst and phagocytic activities were significantly increased after 10 days of exposition compared to the control fish. Overall, As-exposure in the seabream affects the immune system. How this might interfere with fish biology, aquaculture management or human consumers warrants further investigations. This paper describes, for the first time, the immunotoxicological effects of arsenic exposure in the gilthead seabream

  7. Immunotoxicological effects of inorganic arsenic on gilthead seabream (Sparus aurata L.)

    Energy Technology Data Exchange (ETDEWEB)

    Guardiola, F.A.; Gónzalez-Párraga, M.P.; Cuesta, A. [Department of Cell Biology and Histology, Faculty of Biology, Campus Regional de Excelencia Internacional “Campus Mare Nostrum”, University of Murcia, 30100 Murcia (Spain); Meseguer, J. [Department of Cell Biology and Histology, Faculty of Biology, Campus Regional de Excelencia Internacional “Campus Mare Nostrum”, University of Murcia, 30100 Murcia (Spain); Department of Agricultural Chemistry, Geology and Pedology, Faculty of Chemistry, Campus Regional de Excelencia Internacional “Campus Mare Nostrum”, University of Murcia, 30100 Murcia (Spain); Martínez, S.; Martínez-Sánchez, M.J.; Pérez-Sirvent, C. [Department of Agricultural Chemistry, Geology and Pedology, Faculty of Chemistry, Campus Regional de Excelencia Internacional “Campus Mare Nostrum”, University of Murcia, 30100 Murcia (Spain); Esteban, M.A., E-mail: aesteban@um.es [Department of Cell Biology and Histology, Faculty of Biology, Campus Regional de Excelencia Internacional “Campus Mare Nostrum”, University of Murcia, 30100 Murcia (Spain)

    2013-06-15

    Highlights: •Short exposure to arsenic increases the hepato-somatic index and produces histopathological alterations in the liver. •Arsenic is bioaccumulated in the liver of gilthead seabream but no in the muscle. •Arsenic-exposure affects the innate immune system in the gilthead seabream. •Ten days of exposure to As enhances the immune parameters. -- Abstract: Arsenic (As) has been associated with multitude of animal and human health problems; however, its impact on host immune system has not been extensively investigated. In fish, there are very few works on the potential risks or problems associated to the presence of arsenic. In the present study we have evaluated the effects of exposure (30 days) to sub-lethal concentrations of arsenic (5 μM As{sub 2}O{sub 3}) in the teleost fish gilthead seabream (Sparus aurata), with special emphasis in the innate immune response. The arsenic concentration was determined using atomic fluorescence spectrometry (AFS) in liver and muscle of exposed fish showing As accumulation in the liver after 30 days of exposure. The hepatosomatic index was increased at significant extent after 10 days but returned to control values after 30 days of exposure. Histological alterations in the liver were observed including hypertrophy, vacuolization and cell-death processes. Focusing on the immunological response, the humoral immune parameters (seric IgM, complement and peroxidase activities) were no affected to a statistically significant extent. Regarding the cellular innate parameters, head-kidney leucocyte peroxidase, respiratory burst and phagocytic activities were significantly increased after 10 days of exposition compared to the control fish. Overall, As-exposure in the seabream affects the immune system. How this might interfere with fish biology, aquaculture management or human consumers warrants further investigations. This paper describes, for the first time, the immunotoxicological effects of arsenic exposure in the

  8. Assessment of gold nanoparticle effects in a marine teleost (Sparus aurata) using molecular and biochemical biomarkers.

    Science.gov (United States)

    Teles, M; Fierro-Castro, C; Na-Phatthalung, P; Tvarijonaviciute, A; Trindade, T; Soares, A M V M; Tort, L; Oliveira, M

    2016-08-01

    Gold nanoparticles (AuNP) are increasingly employed in a variety of applications and are likely to be increasing in the environment, posing a potential emerging environmental threat. Information on possible hazardous effects of engineered nanoparticles is urgently required to ensure human and environmental safety and promote the safe use of novel nanotechnologies. Nevertheless, there is a lack of comprehensive knowledge on AuNP effects in marine species. The present study aimed to assess AuNP effects in a marine teleost, Sparus aurata, by combining endpoints at different biological levels (molecular and biochemical). For that purpose, fish were exposed via water for 96h to 4, 80 and 1600μgL(-1) of AuNP (∼40nm) coated with citrate or polyvinylpyrrolidone (PVP). Results revealed a significant impact of AuNP-PVP in the hepatic expression of antioxidant, immune and apoptosis related genes. Total oxidative status was increased in plasma after exposure to the lowest concentration of AuNP-PVP, although without altering the total antioxidant capacity. Furthermore, AuNP did not induce significant damage in the liver since the activity of neither hepatic indicator (aspartate aminotransferase and alkaline phosphatase) increased. Overall, the present study demonstrated that AuNP, even with a biocompatible coating is able to alter oxidative status and expression of relevant target genes in marine fish. Another important finding is that effects are mainly induced by the lowest and intermediate concentrations of the PVP coated AuNP revealing the importance of different coatings. PMID:27267391

  9. Tamoxifen persistently disrupts the humoral adaptive immune response of gilthead seabream (Sparus aurata L.).

    Science.gov (United States)

    Rodenas, M C; Cabas, I; Abellán, E; Meseguer, J; Mulero, V; García-Ayala, A

    2015-12-01

    There is increasing concern about the possible effect of pharmaceutical compounds may have on the fish immune system. Bath exposition of 17α-ethynylestradiol (EE2), a synthetic estrogen used in oral contraceptives, altered the immune response of the gilthead seabream (Sparus aurata L.), a marine hermaphrodite teleost. Tamoxifen (Tmx) is a selective estrogen-receptor modulator used in hormone replacement therapy, the effects of which are unknown in fish immunity. This study aims to investigate the effects of dietary administration of EE2 (5 μg/g food) and Tmx (100 μg/g food) on the immune response of gilthead seabream, and the capacity of the immune system to recover its functionality after a recovery period. The results show for the first time the reversibility of the effect of EE2 and Tmx on the fish immune response. Tmx promoted a transient alteration in hepatic vitellogenin gene expression of a different magnitude to that produced by EE2. Both, EE2 and Tmx inhibited the induction of interleukin-1β gene expression while reversed the inhibition of ROI production in leukocytes following vaccination. However, none of these effects were observed after ceasing EE2 and Tmx exposure. EE2 and Tmx stimulated the antibody response of vaccinated fish although Tmx, but not EE2, altered the antibody response and modulated the percentage of IgM(+) B lymphocytes of vaccinated fish during the recovery phase. Taken together, our results suggest that EE2 and Tmx might alter the capacity of fish to appropriately respond to infection and show that Tmx has a long-lasting effect on humoral adaptive immunity. PMID:26234710

  10. Evaluation of gemfibrozil effects on a marine fish (Sparus aurata) combining gene expression with conventional endocrine and biochemical endpoints.

    Science.gov (United States)

    Teles, M; Fierro-Castro, C; Na-Phatthalung, P; Tvarijonaviciute, A; Soares, A M V M; Tort, L; Oliveira, M

    2016-11-15

    The information on the potential hazardous effects of gemfibrozil (GEM) on marine fish is extremely scarce. In the current study, molecular, endocrine and biochemical parameters were assessed in Sparus aurata after 96h waterborne exposure to a GEM concentration range. Hepatic mRNA levels of target genes known to be regulated via peroxisome proliferator-activated receptor α (pparα) in mammals, such as apolipoprotein AI (apoa1) and lipoprotein (lpl) were significantly increased, without a concomitant activation of the ppar pathways. GEM (15μgL(-1)) induced an upregulation in mRNA levels of interleukin 1β (il1β), tumour necrosis factor-α (tnfα) and caspase 3 (casp3), suggesting an activation of proinflammatory processes in S. aurata liver. However, mRNA levels of genes related with the antioxidant defence system and cell-tissue repair were unaltered under the tested experimental conditions. Higher levels of GEM induced a cortisol rise, an indication that it is recognized as a stressor by S. aurata. Cortisol levels and the mRNA levels of il1β, tnfα and casp3 may be suggested as potential biomarkers of GEM effects in marine fish. PMID:27474849

  11. Lipid hydrolysis and oxidation in farmed gilthead seabream (Sparus aurata) slaughtered and chilled under different icing conditions

    OpenAIRE

    Pena, Javier; Álvarez, Victoria; Aubourg, Santiago P.

    2010-01-01

    This work focuses on the slaughtering and chilled storage (up to 14 days) related to the commercialization of fresh farmed gilthead seabream (Sparus aurata). A slurry ice (SI) system was applied and evaluated in comparison to traditional flake ice (FI). Lipid hydrolysis and oxidation were analyzed and compared to sensory acceptance and trimethylamine (TMA) formation. An important quality loss could be assessed in fish during slaughtering and chilling storage, according to sensory assessment a...

  12. Skin healing and scale regeneration in fed and unfed sea bream, Sparus auratus

    Directory of Open Access Journals (Sweden)

    Canario Adelino VM

    2011-10-01

    Full Text Available Abstract Background Fish scales are an important reservoir of calcium and phosphorus and together with the skin function as an integrated barrier against environmental changes and external aggressors. Histological studies have revealed that the skin and scales regenerate rapidly in fish when they are lost or damaged. In the present manuscript the histological and molecular changes underlying skin and scale regeneration in fed and fasted sea bream (Sparus auratus were studied using a microarray 3 and 7 days after scale removal to provide a comprehensive molecular understanding of the early stages of these processes. Results Histological analysis of skin/scales revealed 3 days after scale removal re-epithelisation and formation of the scale pocket had occurred and 53 and 109 genes showed significant up or down-regulation, respectively. Genes significantly up-regulated were involved in cell cycle regulation, cell proliferation and adhesion, immune response and antioxidant activities. 7 days after scale removal a thin regenerated scale was visible and only minor changes in gene expression occurred. In animals that were fasted to deplete mineral availability the expression profiles centred on maintaining energy homeostasis. The utilisation of fasting as a treatment emphasised the competing whole animal physiological requirements with regard to barrier repair, infection control and energy homeostasis. Conclusions The identification of numerous genes involved in the mitotic checkpoint and cell proliferation indicate that the experimental procedure may be useful for understanding cell proliferation and control in vertebrates within the context of the whole animal physiology. In response to skin damage genes of immune surveillance were up-regulated along with others involved in tissue regeneration required to rapidly re-establish barrier function. Additionally, candidate fish genes were identified that may be involved in cytoskeletal re

  13. Lipid hydrolysis and oxidation in farmed gilthead seabream (Sparus aurata slaughtered and chilled under different icing conditions

    Directory of Open Access Journals (Sweden)

    Pena, Javier

    2010-06-01

    Full Text Available This work focuses on the slaughtering and chilled storage (up to 14 days related to the commercialization of fresh farmed gilthead seabream (Sparus aurata. A slurry ice (SI system was applied and evaluated in comparison to traditional flake ice (FI. Lipid hydrolysis and oxidation were analyzed and compared to sensory acceptance and trimethylamine (TMA formation. An important quality loss could be assessed in fish during slaughtering and chilling storage, according to sensory assessment and TMA formation. However, lipid damage development was found relatively low, in agreement to the different lipid quality indexes checked (lipid hydrolysis; primary, secondary and tertiary lipid oxidation. No development of rancid odor and no polyunsaturated fatty acid losses were detected. The employment of SI as a slaughtering and chilling strategy was found useful to inhibit quality loss in gilthead seabream resulting in a shelf life increase and a TMA and free fatty acid formation inhibition.Este trabajo estudia el sacrificio y conservación en hielo (hasta 14 días de dorada (Sparus aurata. Para ello, se aplicó hielo líquido (SI de forma comparativa con hielo tradicional (FI en escamas. Se analizó el desarrollo de hidrólisis y oxidación lipídicas, procediéndose a su comparación con la evaluación sensorial y la formación de trimetilamina (TMA. Se observó una importante pérdida de calidad de acuerdo con la valoración sensorial y la formación de TMA. Sin embargo, la alteración lipídica fue baja, a tenor de los índices ensayados (hidrólisis lipídica; oxidación lipídica primaria, secundaria y terciaria. No se detectaron desarrollo de olor rancio ni pérdida de ácidos grasos poliinsaturados. El empleo de SI en esta especie resultó ser útil al objeto de inhibir la pérdida de calidad; así, se observó un incremento en el tiempo de vida útil y una inhibición en la formación de TMA y ácidos grasos libres.

  14. Comparative evaluation of sunflower oil and linseed oil as dietary ingredient for gilthead seabream (Sparus aurata fingerlings

    Directory of Open Access Journals (Sweden)

    Wassef Elham A.

    2015-03-01

    Full Text Available A feeding trial was conducted to define the optimal mixtures of either sunflower oil (SFO or linseed oil (LO with fish oil (FO, in fish meal (FM based diets for gilthead seabream (Sparus aurata fingerling, without significant effect on fish performance, fatty acid composition and liver structure. The trial lasted nine weeks with 420 fish (~4.0 g testing seven isonitrogenous (~48% CP and isolipidic (~18% L diets contained three incremental inclusions of either SFO or LO (40, 48, 56 g kg-1 and the only-fish oil control (CTRL diet. Results showed that the combination of 32 g fish oil plus 48 g of either SFO or LO kg-1 diet as the lipid source had performed the best among all. Fatty acid (FA composition of muscle lipids evidenced that specific fatty acids were selectively retained or utilized. Diet induced- changes in hepatic morphology with vegetable oil inclusion level were further described. Linolenic acid (α-LNA, n-3 had led to less pronounced steatosis symptoms than linoleic acid (LOA, n-6 in liver cells. This study provides sound support for the use of preferably sunflower oil then linseed oil as a complementary lipid resource (48 g kg diet-1 with marine fish oil in gilthead seabream fingerling diets. Therefore, represents novel data on the potential of using sunflower oil (SFO as a possible dietary partial substitute of fish oil for the species.

  15. Importance of prebiotics in aquaculture as immunostimulants. Effects on immune system of Sparus aurata and Dicentrarchus labrax.

    Science.gov (United States)

    Carbone, Donatella; Faggio, Caterina

    2016-07-01

    Infectious diseases in fish represent a major problem for the aquaculture field as they produce extensive damages and loss. Over the last few years, with increased development of the aquaculture industry, different methods have been used to contrast these pathologies. Common interest has led to the use of components (as additives in diets) that could contrast diseases without causing any negative impact on the environment. These components are represented by prebiotics, probiotics, and plant extracts. In this review, the effects of prebiotics are described. Prebiotics are indigestible fibres fermented by gut enzymes and commensal bacteria, whose beneficial effects are due to the by-products generated from fermentation. The influence of pre-biotics on the immune system of fish is called immunosaccharides. Mannanoligosaccharides (MOS), Fructooligosaccharides (FOS) and Inulin act at different levels in the innate immune response. For example, through phagocytosis, lysozyme activity, and the complement system activity, an increase in fish growth and an amelioration of their health status is brought about. In this review, the use of prebiotics in aquaculture, such as immunostimulants, has been highlighted: particularly in two teleost fish species, Sparus aurata and Dicentrarchus labrax. The results demonstrate that the road is still long and further studies are required, but the use of prebiotics, individually or coupled together, can open the doors to pioneering a new model of alternative components to antimicrobial agents. PMID:27074444

  16. Gyrodactylus orecchiae sp. n. (Monogenea: Gyrodactylidae) from farmed populations of gilthead seabream (Sparus aurata) in the Adriatic Sea.

    Science.gov (United States)

    Paladini, Giuseppe; Cable, Joanne; Fioravanti, Maria L; Faria, Patricia J; Di Cave, David; Shinn, Andrew P

    2009-03-01

    Gyrodactylus orecchiae sp. n. (Monogenea, Gyrodactylidae) is described from the skin, fins, eyes and gills of juvenile Sparus aurata L. (gilthead seabream) following two outbreaks of gyrodactylosis amongst stocks held in inshore floating cages on the Adriatic coast of Albania and Croatia. Fish were heavily infected (1000+ gyrodactylids/fish) with G. orecchiae which reportedly resulted in approximately 2-10% mortality amongst the infected stock. Morphologically, the haptoral hooks of G. orecchiae most closely resemble those of Gyrodactylus arcuatus Bychowsky, 1933 in the approximate shape of the ventral bar with its pronounced ventral bar processes and marginal hook sickles which possess a square line to the inner edge of the sickle blade and large rounded heels. The marginal hooks are also morphologically similar to those of Gyrodactylus quadratidigitus Longshaw, Pursglove et Shinn, 2003 and Gyrodactylus colemanensis Mizelle et Kritsky, 1967, but G. orecchiae can be readily discriminated from all three species by the characteristic infolding of the hamuli roots and the shape of the marginal hook sickle. Molecular sequencing of the ITS1, 5.8S, ITS2 regions (513+157+404 bp, respectively) of G. orecchiae and alignment with other gyrodactylids for which these same genomic regions have been determined, suggests that this is a new species. No similarities were found when the ITS1 region of G. orecchiae was compared with 84 species of Gyrodactylus available on GenBank. PMID:19391328

  17. Enteromyxum leei (Myxozoa: Myxosporea) infection in gilthead sea bream (Sparus aurata): Immune-related studies

    OpenAIRE

    Estensoro Atienza, Itziar

    2013-01-01

    La presente tesis doctoral estudia diversos aspectos de la enteromixosis de la dorada, relacionados principalmente con la transmisión y la caracterización del parásito, con los factores que afectan a su establecimiento en el hospedador y a la interacción parásito-hospedador, y con la respuesta inmunitaria inducida por el parásito. En primer lugar, se desarrolló un nuevo método de transmisión de E. leei, que mejora los resultados obtenidos por los hasta ahora disponibles. El parásito se tr...

  18. Mercury Accumulation, Structural Damages, and Antioxidant and Immune Status Changes in the Gilthead Seabream (Sparus aurata L.) Exposed to Methylmercury.

    Science.gov (United States)

    Guardiola, F A; Chaves-Pozo, E; Espinosa, C; Romero, D; Meseguer, J; Cuesta, A; Esteban, M A

    2016-05-01

    In aquatic systems, mercury (Hg) is an environmental contaminant that causes acute and chronic damage to multiple organs. In fish, practically all of the organic Hg found is in the form of methylmercury (MeHg), which has been associated with animal and human health problems. This study evaluates the impact of waterborne-exposure to sublethal concentrations of MeHg (10 μg L(-1)) in gilthead seabream (Sparus aurata). Hg was seen to accumulate in liver and muscle, and histopathological damage to skin and liver was detected. Fish exposed to MeHg showed a decreased biological antioxidant potential and increased levels of the reactive oxygen molecules compared with the values found in control fish (nonexposed). Increased liver antioxidant enzyme activities (superoxide dismutase and catalase) were detected in 2 day-exposed fish with respect to the values of control fish. However, fish exposed to MeHg for 10 days showed liver antioxidant enzyme levels similar to those of the control fish but had increased hepato-somatic index and histopathological alterations in liver and skin. Serum complement levels were higher in fish exposed to MeHg for 30 days than in control fish. Moreover, head-kidney leukocyte activities increased, although only phagocytosis and peroxidase activities showed a significant increase after 10 and 30 days, respectively. The data show that 30 days of exposure to waterborne MeHg provokes more significant changes in fish than a short-term exposure of 2 or 10 days. PMID:26906265

  19. Melatonin synthesis under calcium constraint in gilthead sea bream (Sparus auratus L.)

    NARCIS (Netherlands)

    Abbink, W.; Kulczkowska, E.; Kalamarz, H.; Guerreiro, P.M.G.; Flik, G.

    2008-01-01

    Brain or blood plasma melatonin was analysed as a measure for pineal melatonin production in sea bream. Access to calcium was limited by diluting the seawater to 2.5‰ and removing calcium from the diet or by prolonged feeding of vitamin D-deficient diet. Interactions/relations between melatonin and

  20. Acidophilic granulocytes in the gills of gilthead seabream Sparus aurata: evidence for their responses to a natural infection by a copepod ectoparasite.

    Science.gov (United States)

    Lui, Alice; Manera, Maurizio; Giari, Luisa; Mulero, Victoriano; Dezfuli, Bahram Sayyaf

    2013-09-01

    Immunohistochemical and ultrastructural studies were conducted on the gills of gilthead seabream, Sparus aurata L., naturally infected with the copepod ectoparasite Ergasilus lizae (Krøyer, 1863) in order to assess pathology and the host immune cell response. Gills of 56 gilthead seabream were screened for ectoparasites; 36 specimens (64.3%) harbored E. lizae. Intensity of infection was 32.7 ± 8.7 (mean ± SE). Pathological alterations to the gills of the host were more pronounced in close proximity to the copepod site of attachment. The parasite attached to the gills by means of its modified second antennae, occluded the arteries, provoked epithelial hyperplasia and hemorrhages and most often caused lamellar disruption. Numerous granular cells were encountered near the site of E. lizae attachment. In both infected and uninfected gills, the granular cells lay within the filaments and frequently occurred within the connective tissue inside and outside the blood vessels of the filaments. The type of granular cell was identified by immunohistochemical staining by using the monoclonal antibody G7 (mAb G7), which specifically recognizes acidophilic granulocytes (AGs) of S. aurata and with an anti-histamine antibody (as a marker for mast cells, MCs) on sections from 13 uninfected gills and 21 parasitized gills. The use of mAb G7 revealed that, in gills harboring copepods, the number of G7-positive cells (i.e., AGs; 32.9 ± 3.9, mean number of cells per 45,000 μm2 ± SE) was significantly higher than the density of the same cells in uninfected gills (15.3 ± 3.8; ANOVA, P < 0.05). Few histamine-positive granular cells (i.e., MCs) were found in the uninfected and parasitized gills. Here, we show, for the first time in S. aurata infected gills, that AGs rather than MCs are recruited and involved in the response to E. lizae infection in seabream. PMID:23644766

  1. Effect of seaweed supplementation on growth performance, immune and oxidative stress responses in gilthead seabream (Sparus aurata

    Directory of Open Access Journals (Sweden)

    Augusto Cesar dos Santos Queiroz

    2014-06-01

    Full Text Available Seaweeds have important nutraceutical properties, including antioxidant and biological response-modifying qualities. Their dietary supplementation may increase the immune and antioxidant capacity of fish necessary to cope to stressful conditions and minimizing disease outbreaks. There are essentially four major groups of seaweed that can be classified by color, namely green (Chlorophyta, brown/yellow (Phaeophyta, red (Rhodophyta, and blue-green (Cyanophyta. Some green seaweed has active radical scavenging properties. Red and brown have bioactive compounds that play a vital role as antihypertensive and antioxidant. The current study was designed to evaluate the effects of seaweed supplementation on growth performance, immune and oxidative stress responses in gilthead seabream (Sparus aurata. Three seaweed species, each from one of the following groups, Rhodophyta (R, Phaeophyta (P and Chlorophyta (C, were supplemented to the experimental diets at 2.5% and 7.5% and tested against a control diet (Ctrl with no supplementation. In addition to six diets with seaweed in two different levels of supplementation: R2.5%, P2.5%, C2.5%, R7.5%, P7.5% and C7.5%; a diet with a mix (M, supplemented at 7.5% (2.5% of each algae was also tested. A total of 360 seabream fingerlings, 13.07 ± 0.13cm and 31.17 ± 0.63g were allocated in 24 rectangular tanks, 115L capacity each, with a 4L/min water output connected to a recirculation seawater system. Fish were fed twice a day until satiation and water quality (temperature: 20.5 ºC; D.O.: 8.13 mg/L; Ammonia: 0.5 mg/L Nitrite: 0.5 mg/L were daily monitored. At day 40 of the trial, an intermediate sampling was carried out. Standard zootechnical parameters were measured for the growth performance determination. In addition, blood, liver and intestine samples were collected for the evaluation of the immune (lysozyme and peroxidase and oxidative stress (lipid peroxidation, glutathione peroxidase, glutathione reductase

  2. Risk assessment for parasites in cultures of Diplodus puntazzo (Sparidae) in the Western Mediterranean: prospects of cross infection with Sparus aurata.

    Science.gov (United States)

    Sánchez-García, Neus; Raga, Juan Antonio; Montero, Francisco E

    2014-08-29

    The sharpsnout seabream Diplodus puntazzo is of interest in Mediterranean fish farming. Disease is an important problem because parasites can spread quickly in culture conditions and fish often develop high parasite burdens. Here we assess the risk that documented parasites pose to the sustainability of D. puntazzo farming. This study specifically considers metazoan and protist parasites recorded from wild and farmed D. puntazzo in scientific literature. Risk assessment studies involve the identification, characterization and qualitative quantification of the risk in question (parasitoses in this case) and the probability of establishment. We considered the parasite species which may be difficult to manage as a priority for research into potential management strategies. Those parasites which could be transmitted from cultures of gilthead seabream (Sparus aurata) were also included in this study. Four groups of parasites represented a risk to D. puntazzo farming, ranging from moderate to high: Ciliophora, Myxozoa, Monogenea and Copepoda. Three parasite species were considered high risk to D. puntazzo cultures: Amyloodinium sp., Cryptocaryon sp. and Enteromyxum leei. These species were responsible for high mortalities in cultures of these and other fish species. In addition Sparicotyle chrysophrii, Caligus ligusticus and Gnathia vorax entail a moderate risk to D. puntazzo Mediterranean farms. No important episodes have been related to caligids and isopods in Mediterranean sparids, nevertheless they should be properly managed to prevent future problems. PMID:24893693

  3. Preliminary insights into the incorporation of rosemary extract (Rosmarinus officinalis L.) in fish feed: influence on performance and physiology of gilthead seabream (Sparus aurata).

    Science.gov (United States)

    Hernández, A; García García, B; Caballero, M J; Hernández, M D

    2015-08-01

    Gilthead seabream (Sparus aurata) were fed a basal (control) diet and four experimental diets (R600, R1200, R1800 and R2400), containing 600, 1200, 1800 and 2400 mg kg(-1), respectively, of rosemary extract (Rosmarinus officinalis L.). At 4 and 12 weeks from the beginning of the ongrowing period, the fish were sacrificed, blood was drawn to obtain plasma and the liver and intestines were dissected. Growth and feed intake were unaffected by rosemary extract addition. A histological examination of the intestine revealed no differences among the dosages, while the liver showed a sharp decrease in hepatic steatosis in diets supplemented with rosemary extract. Furthermore, plasma alanine aminotransferase was lower with these diets at the end of the ongrowing period. Rosemary extract reduced the plasma levels of glucose and triglycerides on week 4 and glucose and HDL/LDL cholesterol ratio on week 12, suggesting better transport and energy metabolism of the lipids. Overall, the most evident effect of rosemary extract was observed with the 600 mg kg(-1) dose. PMID:25968936

  4. Effects of dietary lipid level on growth and feed utilisation of gilthead seabream (Sparus aurata L. reared at Mediterranean summer temperature

    Directory of Open Access Journals (Sweden)

    Fulvio Mongile

    2014-02-01

    Full Text Available We investigated the effects of different dietary lipid levels on gilthead seabream, Sparus aurata, reared at Mediterranean summer temperature. Sixty fish (average weight 75 g per tank were randomly distributed, in triplicate groups, in a recirculating rearing system (27±1°C and fed ad libitum five isonitrogenous (46% dietary protein diets with increasing lipid level (16, 18, 20, 22 and 24% named D16, D18, D20, D22 and D24, respectively, over 89 days. Specific growth rate and final body weight were not affected by dietary lipid levels. Feed conversion ratio was significantly higher (P≤0.05 in D16 as compared to the other treatments, most likely due to the shortage of dietary energy supply, coped with a significantly higher voluntary feed intake. Consequently, we obtained a significantly lower protein efficiency ratio and gross protein efficiency in D16. Gross lipid efficiency was significantly higher in D16 and D18 than in the other treatments. Biometric parameters and lipase activity in gut content were not influenced by dietary treatments. In conclusion, D18 seems the most suitable diet for gilthead seabream reared at Mediterranean summer temperature, providing both the lowest fish in fish out (FIFO ratio and a protein sparing effect, which makes gilthead seabream’s production economically and environmentally more sustainable.

  5. Glycogenesis and de novo lipid synthesis from dietary starch in juvenile gilthead sea bream (Sparus aurata) quantified with stable isotopes

    DEFF Research Database (Denmark)

    Ekmann, Kim Schøn; Dalsgaard, Anne Johanne Tang; Holm, Jørgen;

    2013-01-01

    , and estimated deposition rates of de novo synthesised lipid originating from starch ranged from 18·7 to 123·7 mg/kg biomass per d. Dietary treatments did not significantly affect growth, feed performance or body composition of the fish, while the hepatosomatic index and glycogen content of whole fish...... synthesis and deposition under the specified experimental conditions...

  6. Morphological characterization of the oral cavity of the gilthead seabream (Sparus aurata) with emphasis on the teeth-age adaptation.

    Science.gov (United States)

    Elgendy, Samir A A; Alsafy, Mohamed A M; Tanekhy, Mahmoud

    2016-03-01

    Gilthead sea bream with different age groups that collected from Seawater fisheries, Ismailia Governorate, Egypt, were examined by gross anatomy and scanning electron microscopy to assess the morphological characteristics of the oral cavity. Teeth patterns showed that the gilthead sea bream is adapted to the feeding pattern according to age development, as it modified from spiny form teeth in young fishes to obelisk-like teeth and flat dome-like teeth in growing fishes, with differentiation of teeth into three pairs of canine and conical teeth, that later differentiated to small and large flat teeth. With development, the apical pouch also showed morphological differentiation from curve-like in small fishes to Y-letter shape in medium-sized fishes, which later grow to completely covering the lower jaw in grown adult fishes. Tongue papillae, on the other hand, showed some differentiation being smoother in growing fishes than adult ones. Consistent with development differentiation, the palatine region of young fishes appeared separated from the palate by deep palatine fissure, while that the same palatine region was continuous with the palate with a remnant of palatine fissure as shallow groove was noticed in grown big fishes. Taste buds were characterized in the oral cavity of small and large fishes however in large fishes; the taste buds were more prominent especially at the palate and palatine folds. These and other morphological features of the oral cavity and the feeding habits in small and large gilthead sea bream fishes were recorded. Microsc. Res. Tech. 79:227-236, 2016. © 2016 Wiley Periodicals, Inc. PMID:26791335

  7. Myostatin-2 gene structure and polymorphism of the promoter and first intron in the marine fish Sparus aurata: evidence for DNA duplications and/or translocations

    Directory of Open Access Journals (Sweden)

    Funkenstein Bruria

    2011-02-01

    Full Text Available Abstract Background Myostatin (MSTN is a member of the transforming growth factor-ß superfamily that functions as a negative regulator of skeletal muscle development and growth in mammals. Fish express at least two genes for MSTN: MSTN-1 and MSTN-2. To date, MSTN-2 promoters have been cloned only from salmonids and zebrafish. Results Here we described the cloning and sequence analysis of MSTN-2 gene and its 5' flanking region in the marine fish Sparus aurata (saMSTN-2. We demonstrate the existence of three alleles of the promoter and three alleles of the first intron. Sequence comparison of the promoter region in the three alleles revealed that although the sequences of the first 1050 bp upstream of the translation start site are almost identical in the three alleles, a substantial sequence divergence is seen further upstream. Careful sequence analysis of the region upstream of the first 1050 bp in the three alleles identified several elements that appear to be repeated in some or all sequences, at different positions. This suggests that the promoter region of saMSTN-2 has been subjected to various chromosomal rearrangements during the course of evolution, reflecting either insertion or deletion events. Screening of several genomic DNA collections indicated differences in allele frequency, with allele 'b' being the most abundant, followed by allele 'c', whereas allele 'a' is relatively rare. Sequence analysis of saMSTN-2 gene also revealed polymorphism in the first intron, identifying three alleles. The length difference in alleles '1R' and '2R' of the first intron is due to the presence of one or two copies of a repeated block of approximately 150 bp, located at the 5' end of the first intron. The third allele, '4R', has an additional insertion of 323 bp located 116 bp upstream of the 3' end of the first intron. Analysis of several DNA collections showed that the '2R' allele is the most common, followed by the '4R' allele, whereas the '1R

  8. Utilization of desalinated b rackish water resi dues for cultivation of the m a rine fish species, Dicentrarchus labra x, Sparus aurata, and Sciaenops ocellatus

    Directory of Open Access Journals (Sweden)

    Mutaz A. Al - Qutob

    2013-05-01

    Full Text Available The utilization of brackish water residues from desalination units for fish mariculture inPalestine could represent an environmental friendly alternative of disposing brine water which could havean adverse effect on wild life. In this study, three juvenile marine fish species (Gilt-head bream S. aurata, European sea bass D. labrax, and red drum S. ocellatus with average weights of 0.7-4.9 g, wereacclimated and reared in brine brackish water residues collected from Jericho desalination units withsalinities of 6.5 ‰ and 11 ‰ diluted sea water as control for 3-7 months. Fish were fed 56 % richprotein diet. Brine brackish water contained high levels of CL-1 (3369 mgL-1, Na+1(3735 mgL-1, K+1(300mgL-1, SO4 -2 (716 mgL-1 with the divalent ions Mg+2 (57.3 mgL-1 and Ca+2 (276 mgL-1.Theexperimental well showed more than the maximum allowable concentration of Cr (14.49 μgL-1, Ag (5.3μgL-1 and Mn (27.88 μgL-1 for water quality of fisheries use. The sea bass with an average weight of0.76 g (at a salinity of 6.5 ‰ showed an acceptable growth performance parameters and reached apercentage weight gain (% WG of 6345.23 % and a survival rate of 77.5 % compared to control groupsat 11 ‰ that reached at the same time a % WG of 6543.78 % and a survival rate of 82 % after 30weeks. The red drum juveniles reached a % WG of 2661.6 % and 2673.92 % after 15 weeks at 6.5 ‰and 11 ‰ respectively while sea bream reached a % WG of 241.63 % and 772.44 % after 15 weeks at6.5 ‰ and 11 ‰ respectively. Survival rate was only 5 % at both salinities for the two species. In afurther study sea bass fingerlings with an average weight of 20.5 g were reared in brackish water of 6.5‰ salinity for 7 weeks and were fed superior fish meal with fish oil. They received diets of 2.1 %, 3.0%, and 4.0 % of body weight. Sea bass fingerlings which received diets of 2.1 %, had the highest FCE(0.82 and PER (1.46 with the lowest FCR (1.22. These results confirm that the most

  9. Development and validation of an HPLC method for the determination of six penicillin and three amphenicol antibiotics in gilthead seabream (Sparus Aurata) tissue according to the European Union Decision 2002/657/EC.

    Science.gov (United States)

    Evaggelopoulou, Evaggelia N; Samanidou, Victoria F

    2013-02-15

    A confirmatory high performance liquid chromatography method for the determination of six penicillin antibiotics and three amphenicol antibiotics in gilthead seabream (Sparus Aurata) tissue was developed. Ampicillin (AMP), penicillin G (PG), penicillin V (PV), oxacillin (OXA), cloxacillin (CLO), dicloxacillin (DICLO), thiamphenicol (TAP), florfenicol (FFC) and chloramphenicol (CAP) were separated on an Inertsil, C(8) (250×4 mm, 5 μm) column by gradient elution with a mobile phase consisting of ammonium acetate 0.05 M and acetonitrile at 25°C. Diode array detection with monitoring at 225 nm (for the determination of AMP, PG, PV, TAP and FFC), 240 nm (for OXA, CLO and DICLO) and 278 nm (for CAP) was applied. Examined antibiotics were isolated from gilthead seabream tissue by liquid-liquid extraction and further clean-up was performed by solid phase extraction using Oasis HLB (200 mg/6 mL) cartridges. The developed method was fully validated in terms of selectivity, linearity, accuracy, precision, stability and sensitivity according to the European Union Decision 2002/657/EC. PMID:23194530

  10. Desarrollo de un programa piloto a nivel nacional de mejora genética en dorada (Sparus aurata L.) : estimación de parámetros genéticos de caracteres de crecimiento y calidad e interacción genotipo ambiente

    OpenAIRE

    Lee Montero, Ivonne Carola

    2012-01-01

    [ES] En este estudio, se ha protocolizado la metodología que permite la integración de la selección genética bajo los condicionantes propios del sistema de producción de dorada (Sparus aurata L.) poniéndola en práctica a nivel nacional en colaboración con seis empresas españolas. Se criaron peces descendientes de tres lotes industriales (ICCM: Canarias, CULMASUR S.A.: Andalucía, PISCIMAR S.L.: Valencia), marcados con Passive Integrated Transponder (PIT) y mezclados, en las instala...

  11. Can Early Life-Stages of the Marine Fish Sparus aurata be Useful for the Evaluation of the Toxicity of Linear Alkylbenzene Sulphonates Homologues (LAS C10-C14 and Commercial LAS?

    Directory of Open Access Journals (Sweden)

    M. Hampel

    2002-01-01

    Full Text Available Most commercial household cleaning agents and personal care products contain the anionic surfactant linear alkylbenzene sulphonates (LAS as the active compound. After their use they are discharged, theoretically after adequate wastewater treatment, into receiving waters finally reaching estuaries and coastal waters. Laboratory toxicity tests are useful tools in determining at which concentration a certain wastewater compound becomes hazardous for an existing group of organisms. Early life-stage toxicity tests include exposure during the most sensitive development period of the organism. In fish, this type of assay has shown to predict accurately maximum acceptable toxicant concentration (MATC values (comprised in the range defined by the NOEC and LOEC in fish early life-stage tests. For this reason, larvae of the seabream, Sparus aurata, were exposed to increasing concentrations of LAS homologues (C10-C14 and commercial LAS. Obtained LC50 values ranged between 0.1 and 3.0 mg l-1 and were compared with LC50 values of previous hatching experiments with the same species. Larvae proved to be more sensitive to LAS exposure of individual homologues than eggs, except in the case of commercial LAS. LC50 values can be directly employed to determine their potential risk in a concrete environment with known pollutant concentrations. Dividing the LC50 value with the found homologue concentration and extrapolating with certain security factors proposed by different environmental organisms, potentially hazardous pollutant concentrations may be detected. Average estuarine or coastal LAS concentrations are generally below toxicity limits for this kind of organism, considering that the average alkyl chain length of commercial LAS is 11.6 carbon atoms.

  12. Virulence and molecular typing of Vibrio harveyi strains isolated from cultured dentex, gilthead sea bream and European sea bass.

    Science.gov (United States)

    Pujalte, M J; Sitjà-Bobadilla, A; Macián, M C; Belloch, C; Alvarez-Pellitero, P; Pérez-Sánchez, J; Uruburu, F; Garay, E

    2003-06-01

    Vibrio harveyi was isolated from internal organs or ulcers of diseased and apparently healthy gilthead sea bream (Sparus aurata) and European sea bass (Dicentrarchus labrax) cultured in several fish farms located on the Spanish Mediterranean coast. The prevalence of the bacterium was significantly higher in European sea bass than in gilthead sea bream, and was closely related to the season in both fish species, occurring almost exclusively on warm months (June to November). After phenotypic characterization, a selection of forty five isolates from gilthead sea bream, sea bass, and several isolates previously obtained from common dentex (Dentex dentex) of the same area, were molecularly typed by automated ribotyping and random amplified polymorphic DNA (RAPD) analysis. Cluster analysis of data established 8 RAPD types and 13 ribotypes among wild isolates, and the combination of both techniques allowed to define fourteen different groups and a clear discrimination of all outbreaks and samplings. Several strains isolated from diseased gilthead sea bream and sea bass and also from asymptomatic sea bream, were tested for virulence in both fish species by intracoelomic injection. All the isolates (11) were pathogenic for sea bass, with nine out of the eleven LD50 values ranging from 1.5 x 10(5) to 1.6 x 10(6) cfu/fish. Gilthead sea bream was unaffected by the seven tested strains, even by those more virulent for sea bass, and only one strain caused a 10% mortality at 4.2 x 10(7) cfu/fish. This is the first report on virulence of V. harveyi for sea bass. PMID:12866856

  13. Sparus aurata L. liver EROD and GST activities, plasma cortisol, lactate, glucose and erythrocytic nuclear anomalies following short-term exposure either to 17β-estradiol (E2) or E2 combined with 4-nonylphenol

    International Nuclear Information System (INIS)

    Immature Sparus aurata L. (gilthead seabream) were exposed to 17β-estradiol (E2) 4000 ng/l and to the same E2 concentration mixed with 50,000 ng/l 4-nonylphenol (E2+NP) during 4, 8, 12 and 16 h. E2 availability and E2 plasma level variations were assessed. Liver biotransformation capacity was measured as ethoxyresorufin-O-deethylase (EROD) and glutathione-S-transferase (GST) activities. Plasma cortisol, lactate and glucose were also determined. Genotoxicity was assessed through erythrocytic nuclear anomalies (ENA) frequency. Liver EROD activity significantly decreased during the whole experiment for both treatments, with the exception of 16 h exposure to E2. Liver GST activity was significantly increased after 8 and 12 h of exposure either to E2 or E2+NP. An endocrine disruption expressed as plasma cortisol decrease was observed after 16 h exposure under both tested conditions, concomitantly with a plasma lactate increase. No genotoxic responses, measured as ENA frequency, were detected. Analyzing the E2 water concentration in aquaria without fish it was demonstrated an intense and fast E2 loss, considerably reducing its availability to fish. In the presence of fish, E2 water levels were drastically reduced after 4 h exposure, being this reduction more pronounced in E2 aquarium when compared to E2+NP aquarium. In addition, it was demonstrated a rapid E2 uptake from the water since the highest E2 plasma concentrations were observed after 4 h exposure, followed by a continuous decrease, which became more pronounced between 8 and 12 h of exposure. Furthermore, during the first 8 h exposure to E2 and E2+NP, seabream plasma E2 concentrations were higher than the initial water exposure concentration. Comparing the E2 plasma levels in both seabream-exposed groups, it was clear that its concentration is always higher in E2+NP-treated fish. Despite the previous results, no significant differences were found in the measured responses between E2 and E2+NP

  14. Sparus aurata L. liver EROD and GST activities, plasma cortisol, lactate, glucose and erythrocytic nuclear anomalies following short-term exposure either to 17{beta}-estradiol (E{sub 2}) or E{sub 2} combined with 4-nonylphenol

    Energy Technology Data Exchange (ETDEWEB)

    Teles, M.; Pacheco, M.; Santos, M.A

    2005-01-05

    Immature Sparus aurata L. (gilthead seabream) were exposed to 17{beta}-estradiol (E{sub 2}) 4000 ng/l and to the same E{sub 2} concentration mixed with 50,000 ng/l 4-nonylphenol (E{sub 2}+NP) during 4, 8, 12 and 16 h. E{sub 2} availability and E{sub 2} plasma level variations were assessed. Liver biotransformation capacity was measured as ethoxyresorufin-O-deethylase (EROD) and glutathione-S-transferase (GST) activities. Plasma cortisol, lactate and glucose were also determined. Genotoxicity was assessed through erythrocytic nuclear anomalies (ENA) frequency. Liver EROD activity significantly decreased during the whole experiment for both treatments, with the exception of 16 h exposure to E{sub 2}. Liver GST activity was significantly increased after 8 and 12 h of exposure either to E{sub 2} or E{sub 2}+NP. An endocrine disruption expressed as plasma cortisol decrease was observed after 16 h exposure under both tested conditions, concomitantly with a plasma lactate increase. No genotoxic responses, measured as ENA frequency, were detected. Analyzing the E{sub 2} water concentration in aquaria without fish it was demonstrated an intense and fast E{sub 2} loss, considerably reducing its availability to fish. In the presence of fish, E{sub 2} water levels were drastically reduced after 4 h exposure, being this reduction more pronounced in E{sub 2} aquarium when compared to E{sub 2}+NP aquarium. In addition, it was demonstrated a rapid E{sub 2} uptake from the water since the highest E{sub 2} plasma concentrations were observed after 4 h exposure, followed by a continuous decrease, which became more pronounced between 8 and 12 h of exposure. Furthermore, during the first 8 h exposure to E{sub 2} and E{sub 2}+NP, seabream plasma E{sub 2} concentrations were higher than the initial water exposure concentration. Comparing the E{sub 2} plasma levels in both seabream-exposed groups, it was clear that its concentration is always higher in E{sub 2}+NP-treated fish. Despite

  15. Türkiye'de kültür ve doğal çipura (Sparus aurata l.) populasyonları arasındaki filogenetik farklılıkların mitokondriyal gen bölgelerinin dna dizi analizleri ile tanımlanması

    OpenAIRE

    DOĞANKAYA, Levent

    2010-01-01

      Bu çalışmada, kıyılarımızda bulunan doğal çipura (Sparus aurata)  populasyonları ile balık çiftliklerinde üretilen çipura populasyonlarının filogenetik ilişkilerinin incelenmesi ve bu stoklar arasındaki farklılıkların belirlenmesi amaçlanmıştır.

    Araştırma için Adana, Mersin, Antalya, Bodrum ve İzmir olmak üzere beş istasyon seçilmiştir. Her istasyonda doğal ve kültür balık stoklarından temin edilen örneklerin karaciğerlerinde...

  16. PREVALENCE AND IDENTIFICATION OF VIBRIO SPP. ISOLATED ON AQUACULTURED GILTHEAD SEA BREAM

    Directory of Open Access Journals (Sweden)

    C. Scarano

    2011-01-01

    Full Text Available The aim of the study was to investigate the prevalence of Vibrio spp isolated from gilthead sea bream (Sparus aurata farmed on sea cages and to identify and characterize the pathogen by molecular techniques. Eighty fish were collected from two hatcheries located on the North-Est Sardinian Mediterranean coast, and microbiological analysis were performed on different body parts such as skin, gills, muscle and intestinal tract. Subsequently 100 pure colonies with typical morphology and phenotypic characteristics were selected and submitted to the molecular identification. The analysis on the prevalence of Vibrio spp showed the effect of the hatchery rearing system (P<0.001, of the date of sampling (P<0.001, and of the body part (P<0.001. All the strains selected were confirmed to be members of the genus Vibrio spp by the molecular method/techinique/identification, whereas the rpoA gene sequence analyses allowed to identify 89 strains belonging to the species Vibrio harveyi, 6 to V. diabolicus, 2 to V. parahaemolyticus and 1 to V. mediterranei.

  17. ANÁLISIS DE LA SITUACIÓN ECONÓMICA-FINANCIERA DEL SECTOR PRODUCTOR DE LA DORADA (Sparus aurata L.), LUBINA (Dicentrarchus labrax L.) Y CORVINA (Argyrosomus regius A.), EN EL LITORAL MEDITERRANEO ESPAÑOL

    OpenAIRE

    BENITO ALAS, FERNANDO

    2016-01-01

    [EN] The state of the Spanish aquaculture Sea bream, Sea bass and Meagre produced in fish farms in the Spanish Mediterranean coast is analyzed setting as objective the analysis of the economical and financial situation of companies in the sector to characterize the successful companies. For this project a study was made during the period from 2002 to 2011, on the period before the economical crisis (2002-2007) and also during the crisis (2007-2011), to interpret the evolution of the compa...

  18. Effects of dietary energy density and digestible protein:energy ratio on de novo lipid synthesis from dietary protein in gilthead sea bream (Sparus aurata) quantified with stable isotopes

    DEFF Research Database (Denmark)

    Ekmann, Kim Schøn; Dalsgaard, Anne Johanne Tang; Holm, Jørgen;

    2013-01-01

    MS was applied to quantify the 13C enrichment of whole-body lipid from dietary DP. Between 18·6 and 22·4% of the carbon derived from protein was recovered in the lipid fraction of the fish, and between 21·6 and 30·3% of the total lipid deposited could be attributed to dietary protein. DP retention...

  19. Garlic Bream

    Institute of Scientific and Technical Information of China (English)

    1999-01-01

    Ingredients:One whole bream weighing about750g,100g garlic,3g salt,15g cookingwine,2g pepper,3g MSG,5g sugar,10g soy sauce,5g sesame oil,optionalamount of clear stock,minced scallionand ginger root and 1000g cooking oil(actual consumption only 100g).Method:1.Clean the bream,slit open bothsides,then smear over the soy sauceand soak for a while.2.Heat the wok and add in cookingoil.Put in the bream when the oil is80% hot.Fry the fish until it turnsgolden yellow.Remove.leaving someoil in the wok.3.Stir-fry the scallion and ginger until

  20. 3种滤料生物滤器的挂膜与黑鲷幼鱼循环水养殖效果%Biofilm cultivation with three stuffings and their effect on the growth of young black sea bream, Sparus macrocephalus

    Institute of Scientific and Technical Information of China (English)

    王威; 曲克明; 朱建新; 王海增; 崔正国; 夏斌

    2012-01-01

    young black sea bream, Sparus macrocephalus. We tested the effect of using microbial water purified agents as a strain for three different kinds of fillers (pottery ring, elastic brush, and biochemical cotton fiber) during biofilm cultivation. The bio-film maturation time for the three fillers was 25, 32, and 28 d, respectively. Our results suggested that maintaining high dissolved oxygen concentrations, changing the water, and adding nutrients and NaCOs promoted the growth and propagation of nitrifying bacteria. Conversely, NO2-N concentrations cannot be held at high levels for an extended period because ammonia-oxidizing bacteria and nitrifying bacteria require dissolved oxygen and alkalinity for survival. Young black sea bream grew well in the simple recirculating aquaculture system. After 40 d feeding, the mean body weight and feed conversion rate of black sea bream in the three experimental groups differed significantly from that in the control group (P95%. The activity of serum LSZ and liver SOD was significantly higher in the experimental groups than the control group (P<0.05). Similarly, the quantity and diversity of digestive tract flora was significantly higher in the experimental groups than the control group. We identified several different kinds of beneficial bacteria in the three experimental groups, relative to the control. Our results suggest that these beneficial bacteria are dominant, and thus inhibited the survival of pathogenic bacteria. During circulation of the water, the beneficial bacteria are able to enter the fish body during respiration and ingestion, thereby enhancing the nonspecific immunity of the fish. Our results suggest that different filter materials have different bio-film maturation times under the same operating conditions. A recirculating aquaculture system can not only improve fish growth, but also enhance their immune performance.

  1. Movements of grey mullet Liza aurata and Chelon labrosus associated with coastal fish farms in the western Mediterranean Sea

    OpenAIRE

    P. ARECHAVALA-LOPEZ; Uglem, Ingebrigt; Sanchez-Jerez, P.; Fernandez-Jover, D.; Bayle-Sempere, J. T.; Nilsen, Rune

    2010-01-01

    Grey mullet occur in abundance around sea bream and sea bass farms where they forage on waste fish feed, a behaviour that could modify their natural movement pattern and distribution. In this study, we used visual census to record grey mullet aggregations at fish farms in the western Mediterranean Sea. We also mapped the movements of 2 species (Liza aurata and Chelon labrosus) between farms and adjacent coastal fishing areas, using acoustic telemetry. Grey mullet were frequently observed in t...

  2. Natural abundance of N-15 and C-13 in fish tissues and the use of stable isotopes as dietary protein tracers in rainbow trout and gilthead sea bream

    OpenAIRE

    Beltran, M; Fernandez-borras, J.; Medale, Francoise; Perez-sanchez, J.; Kaushik, Sadasivam; Blasco, J.

    2009-01-01

    For developing efficient diets, two sets of experiments examined whether the use and allocation of dietary protein can be traced by labelling with stable isotopes (N-15 and C-13) in two culture fish (Oncorhynchus mykiss and Sparus aurata). In the first experiment, natural abundance and tissue distribution of these isotopes were determined, by measuring the delta C-13 and delta N-15 values by isotopic ratio mass spectrometry, in fingerlings (14-17 g) adapted to diets differing in the percentag...

  3. Untersuchungen zur Qualität von Doraden auf dem deutschen Markt

    OpenAIRE

    Karl, Horst; Meyer, Carsten

    2002-01-01

    Mediterranean fish species and fish species from other southern countries are retailed increasingly on the German market. The fish is stored in ice eviscerated or as whole fish and imported by truck or by airplane. In the present investigation, the freshness, the sensory quality and the microbiological status was determined of gilthead sea breams (Sparus aurata) from farms in Italy and France and from sea breams (Sparus ehrenbergi) of wild captures from Senegal. It is shown, that farmed gilth...

  4. Daily rhythms of digestive enzyme activity and gene expression in gilthead seabream (Sparus aurata) during ontogeny.

    Science.gov (United States)

    Mata-Sotres, José Antonio; Moyano, Francisco Javier; Martínez-Rodríguez, Gonzalo; Yúfera, Manuel

    2016-07-01

    In order to identify daily changes in digestive physiology in developing gilthead seabream larvae, the enzyme activity (trypsin, lipases and α-amylase) and gene expression (trypsinogen-try, chymotrypsinogen-ctrb, bile salt-activated lipase-cel1b, phospholipase A2-pla2 and α-amylase-amy2a) were measured during a 24h cycle in larvae reared under a 12h light/12h dark photoperiod. Larvae were sampled at 10, 18, 30 and 60days post-hatch. In each sampling day, larvae were sampled every 3h during a complete 24h cycle. The enzyme activity and gene expression exhibited a marked dependent behavior to the light/darkness cycle in all tested ages. The patterns of activity and expression of all tested enzymes were compared to the feeding pattern found in the same larvae, which showed a rhythmic feeding pattern with a strong light synchronization. In the four tested ages, the activities of trypsin, and to a lesser extent lipases and amylase, were related to feeding activity. Molecular expression of the pancreatic enzymes tended to increase during the night, probably as an anticipation of the forthcoming ingestion of food that will take place during the next light period. It follows that the enzymatic activities are being regulated at translational and/or post-translational level. The potential variability of enzyme secretion along the whole day is an important factor to take into account in future studies. A particularly striking consequence of the present results is the reliability of studies based in only one daily sample taken at the same hour of the day, as those focused to assess ontogeny of digestive enzymes. PMID:26987267

  5. Control de l'expressió del gen de la glucoquinasa en fetge de "Sparus Aurata"

    OpenAIRE

    Egea Liria, Miriam

    2007-01-01

    Els peixos carnívors estan adaptats a la utilització d'elevades quantitats de proteïnes. Aquestes són el component essencial de la seva dieta natural. D'altra banda, els peixos carnívors presenten una certa intolerància a la glucosa, ja que tenen una baixa capacitat per aprofitar els carbohidrats de la dieta. Aquesta característica queda reflectida en l'elevada hiperglicèmia postprandial que s'observa en alimentar els peixos carnívors amb dietes d'elevat contingut en carbohidrats. Pel que fa ...

  6. Expression of digestive enzyme precursors under different feeding conditions in Sparus aurata larvae

    OpenAIRE

    Sánchez-Amaya, María Isabel; Yúfera, Manuel; Martínez-Rodríguez, Gonzalo

    2009-01-01

    The onset of feeding is a crucial step during the development of marine fish lar-vae. As with most marine species, the digestive tract is not totally mature at mouth opening, but it develops over the whole larval period (Sánchez-Amaya et al., 2007). The digestive capacity during early development of the digestive sys-tem is provided by the pancreatic enzymes (proteases, lipases and glucosidases) in conjunction with alkaline proteolitic enzymes secreted by the intestine, which are important pr...

  7. Update on Fish Disease Situation in Italy

    OpenAIRE

    Vendramin, Niccolò; Toffan, A.

    2012-01-01

    In this presentation we provide a general overview of the aquatic animal health issues related to the aquaculture sector and wild environment in Italy in 2011.Considering saltwater species European sea bass (Dicentrarchus labrax) and Gilthead sea bream (Sparus aurata) are still the most widely farmed species, nevertheless some “new” candidates are employed often.The first two species represents more than 95% of the total production while the remaining is obtained by different promising candid...

  8. PHYTOPLANKTON ASSEMBLAGES AT FISH FARM IN MASLINOVA BAY (THE ISLAND OF BRAČ)

    OpenAIRE

    Sanda Skejić; Živana Ninčević Gladan; Ivona Marasović

    2012-01-01

    The aim of this study was to establish phytoplankton composition at the sea bream (Sparus aurata) and sea bass (Dicentrarchus labrax) fish farm in the middle Adriatic Sea. The investigation was performed from September 2005 to September 2006 at a station located in Maslinova Bay at the island of Brač. Considering the whole research period, diatoms generally prevailed in terms of abundance while dinoflagellates were particularly abundant in June. Number of species of diatoms in comparison to d...

  9. MERAMOD - predicting the deposition and benthic impact of aquaculture in the Eastern Mediterranean

    OpenAIRE

    Cromey, C.J.; Thetmeyer, Helmut; Lampadariou, N.; Black, K.D.; Kögeler, J.; Karakassis, I.

    2012-01-01

    A model, composed of coupled particle tracking and benthic response modules, for predicting waste solids flux and benthic impacts of gilthead sea bream (Sparus aurata L.) and sea bass (Dicentrarchus labrax L.) aquaculture, was tested at six sites with different hydrodynamics, bathymetries and biomasses in the Aegean and Ionian Seas, Eastern Mediterranean with observations of sediment trap flux and benthic impact indicators. Seven sediment trap validation studies were conducted that varied in ...

  10. Studies on metazoan parasites of two marine fish species of interest for aquaculture: Seriola dumerili and Sparus aurata

    OpenAIRE

    Repullés Albelda, Aigües

    2013-01-01

    0.1. Introducción general La acuicultura ha experimentado un importante aumento durante los últimos 50 años, resultado del incremento global de población y la creciente demanda de proteínas de alta calidad. En los últimos 40 años, la proporción de reservas pesqueras moderadamente explotadas ha descendido progresivamente hasta estabilizarse en el 15% y en la actualidad, la mayoría de los stocks pesqueros están sobreexplotados y (FAO, 2010). Más de la mitad de los productos acuáticos provien...

  11. Gilthead seabream (Sparus aurata) Mx gene promoters respond differentially to IPNV and VHSV infections in RTG-2 cells.

    Science.gov (United States)

    González-Mariscal, J A; Fernández-Trujillo, M A; Alonso, M C; García-Rosado, E; Álvarez, M C; Béjar, J

    2016-03-01

    The understanding of virus-host interactions relies on the knowledge of the regulatory mechanisms of the type I interferon (IFN I)-stimulated genes (ISGs). Among ISGs, those coding Mx proteins play a main role due to their direct antiviral activity. The study of these genes in gilthead seabream is interesting, since this species displays high natural resistance to viral diseases, being asymptomatic carrier of infectious pancreatic necrosis virus (IPNV) and viral haemorrhagic septicaemia virus (VHSV). Gilthead seabream has three Mx genes (Mx1, Mx2, and Mx3), encoding proteins with a wide spectrum of antiviral activity. The structure of the three promoters (pMx1, pMx2 and pMx3) has been previously disclosed, and their response to poly I:C in RTG-2 cells characterized. To further analyze these promoters, their response to two viral infections has been evaluated in the present study. For that purpose, RTG-2 cells transiently transfected with the luciferase gene under the control of each promoter were inoculated with either IPNV or VHSV at two different doses. The highest and lowest fold induction values were recorded for pMx2 and pMx3, respectively. The promoter induction was always stronger after VHSV inoculation than in IPNV-inoculated cells. In addition, the higher dose of VHSV tested induced higher response of the three promoters, whereas in IPNV-infected cells the highest induction was recorded after inoculation with the lower viral dose. To further study the response of the Mx2 promoter, RTG-2 cells stably transfected with the luciferase gene under the control of pMx2 were stimulated with poly I:C and subsequently infected with IPNV or VHSV. Interestingly, IPNV infection inhibited the induction caused by poly I:C, suggesting an antagonistic activity of IPNV on Mx2 transcription. In contrast, VHSV infection did not alter the response triggered by poly I:C. These results highlight the specific regulation that controls the activity of each promoter, and support the existence of complex interactions between host cells, specific Mx promoters, and viruses, which are responsible for the final outcome of a viral infection. PMID:26964720

  12. Metabolic fingerprinting of gilthead seabream (Sparus aurata liver to track interactions between dietary factors and seasonal temperature variations

    Directory of Open Access Journals (Sweden)

    Tomé S. Silva

    2014-08-01

    Full Text Available Farmed gilthead seabream is sometimes affected by a metabolic syndrome, known as the “winter disease”, which has a significant economic impact in the Mediterranean region. It is caused, among other factors, by the thermal variations that occur during colder months and there are signs that an improved nutritional status can mitigate the effects of this thermal stress. For this reason, a trial was undertaken where we assessed the effect of two different diets on gilthead seabream physiology and nutritional state, through metabolic fingerprinting of hepatic tissue. For this trial, four groups of 25 adult gilthead seabream were reared for 8 months, being fed either with a control diet (CTRL, low-cost commercial formulation or with a diet called “Winter Feed” (WF, high-cost improved formulation. Fish were sampled at two time-points (at the end of winter and at the end of spring, with liver tissue being taken for FT-IR spectroscopy. Results have shown that seasonal temperature variations constitute a metabolic challenge for gilthead seabream, with hepatic carbohydrate stores being consumed over the course of the inter-sampling period. Regarding the WF diet, results point towards a positive effect in terms of performance and improved nutritional status. This diet seems to have a mitigating effect on the deleterious impact of thermal shifts, confirming the hypothesis that nutritional factors can affect the capacity of gilthead seabream to cope with seasonal thermal variations and possibly contribute to prevent the onset of “winter disease”.

  13. Enrichment of gilthead seabream (Sparus aurata L.) diet with palm fruit extracts and probiotics: Effects on skin mucosal immunity.

    Science.gov (United States)

    Cerezuela, Rebeca; Guardiola, Francisco A; Cuesta, Alberto; Esteban, M Ángeles

    2016-02-01

    Fish skin mucus contains numerous immune substances still poorly studied. To date, there are no studies regarding the possible influence of dietary supplements on such important substances. In the present work, a commercial diet used as control diet was enriched with: 1) probiotic Shewanella putrefaciens (Pdp11 diet, 10(9) cfu g(-1)); 2) probiotic Bacillus sp. (Bacillus diet, 10(9) ufc g(-1)); 3) aqueous date palm fruits extracts (DPE diet, 4%), and 4) a combination of Pdp11 + Bacillus sp + aqueous DPE (Mix diet). After 2 and 4 weeks of the feeding trial, enzymatic activities (proteases, antiproteases and peroxidases), IgM levels and terminal carbohydrates abundance were determined in skin mucus. In addition, the expression of certain immune related genes was evaluated in the skin. Our results demonstrated the significant alteration of the terminal carbohydrate abundance in skin mucus. Carbohydrates more affected by experimental diets were N-acetyl-galactosamine, N-acetyl-glucosamine, galactose, mannose, glucose and fucose. IgM, peroxidase activity and protease were also significantly higher in fish fed enriched diets. For last, an important up-regulation on the immune related gene studied on the skin was also detected. Present findings provide robust evidence that fish skin mucosal immunity can be improved by the diet. PMID:26712151

  14. PHYTOPLANKTON COMPOSITION AT THE FISH AND SHELLFISH FARM IN THE KALDONTA BAY (CRES ISLAND)

    OpenAIRE

    Marija Tomec

    2004-01-01

    The Kaldonta Bay is situated at the south–western coast of the Cres island in the Lošinj channel, rather protected from larger influence of general sea water current. In the Bay there are installed 44 floating cages of 5 by 10 m dimensions. The cages are used for the culture of about 70 tons of sea water fish: gilthead sea bream (Sparus aurata), sea bass (Dicentrarchus labrax), sharp–snouted sparus (Diplodus puntazzo) and dentex (Dentex dentex). Besides some physico–chemical parameters (sea w...

  15. Induction of apoptosis in sea bream fibroblasts by Vibrio harveyi haemolysin and evidence for an anti-apoptotic role of heat shock protein 70.

    Science.gov (United States)

    Deane, E E; Jia, A; Qu, Z; Chen, J-X; Zhang, X-H; Woo, N Y S

    2012-04-01

    In this study, we exposed black sea bream, Mylio macrocephalus (Basilewsky), fibroblast (BSF) and silver sea bream, Sparus sarba Forsskål, fibroblast (SSF) cell lines to a recombinant Vibrio harveyi haemolysin (VHH) and investigated mechanisms involved in apoptosis. A decrease in mitochondrial membrane potential, followed by an increase in caspase 3 activity, occurred within 2-8 h of VHH exposure, in both cell lines; however, VHH did not alter cellular levels of reactive oxygen species. As heat shock protein 70 (HSP70) is known to prevent the onset of apoptosis in certain mammalian cells, we aimed to test whether such a protective effect is operative in VHH-exposed fibroblasts. The amounts of HSP70 were elevated in SSF and BSF via an acute heat shock or an acute heat shock followed by a 6 h recovery. It was found that the VHH-mediated reduction in mitochondrial membrane potential was suppressed in cells that had a 6 h post-heat shock recovery, and the protective effect of heat shock-induced HSP70 was attenuated following treatment of cells with the HSP70 inhibitor, quercetin. This study demonstrates how haemolysin causes cell death via induction of apoptosis and provides evidence as to the role of HSP70 as an anti-apoptotic factor. PMID:27081923

  16. Acute toxicity modeling of rainbow trout and silver sea bream exposed to waterborne metals.

    Science.gov (United States)

    Liao, C M; Lin, M C

    2001-01-01

    Of three proposed acute toxicity models, the uptake-depuration (UD) model, the time-integrated concentration (TIC) model, and the concentration-time (CT) model are derived and verified with acute toxicity data to estimate the internal residues of waterborne metals in fish as a function of a few constants and variables. The main factors are the exposure time, the external exposure concentration, the bioconcentration factor (BCF), and the depuration rate constant (k2). The UD model is based on the concept of residue levels at the cell membrane well correlating with the whole-body concentrations, whereas the TIC and the CT models are based on the idea of irreversible inhibition of the enzyme acetylcholinesterase (AChE) governing the metal acute toxicity in that metals in the entire fish or in the aqueous phase can be described by the critical area under the time-concentration curve that is associated with a critical TIC of toxicant in the target tissue. A highly significant correlation (r2 > 0.9) was found between predictions and LC50(t) data for both the TIC and the CT models, indicating successfully describe 4- to 18-d LC50(t) data of arsenic (As), cobalt (Co), copper (Cu), and Co/Cu mixture in rainbow trout (Oncorhyuchus mykiss) and of Cu in fingerlings and subadults of silver sea bream (Sparus sarba). The time-dependent lethal internal concentration at the site of action that causes 50% mortality is also predicted for a given compound and species. It concludes that the TIC and the CT models can be applied to regulate the acute toxicity and to estimate incipient LC50 values and internal residues of waterborne metals in fish. PMID:11501285

  17. hybridization behaviour between two common European cyprinid fish species - silver bream Blicca bjoerkna and common bream Abramis brama - in a controlled environment

    OpenAIRE

    Nzau Matondo, Billy; Ovidio, Michaël; Philippart, Jean-Claude; Poncin, Pascal

    2009-01-01

    The egg release–mating comparison, heterospecific matings and mating success under two hybridization conditions – (i) mixing one sex per species and (ii) mixing both sexes from each species – were investigated to determine whether silver bream Blicca bjoerkna and common bream Abramis brama can hybridize in nature. The results revealed that non-matings in hybridization experiments of silver bream females × common bream males can be explained by territorial and aggressiveness act...

  18. State of bream populations in reconstructed water bodies of Moldova

    OpenAIRE

    Adrian USATYI; Marin USATYI; Oleg CREPIS; Mihail SHATUNOVSKII; Alexandr BOBYREV; Aurel CEBANU

    2010-01-01

    Bream, Abramis brama L., is a key species in fish communities of Moldovian Rivers and a main component of bottom food chains of river and lake ecosystems. With the reconstruction of water bodies, mainly for hydroelectric power stations needs, the ecological conditions changed substantially, which results in modifications in population state of biota. The negative effects of water bodies’ reconstruction upon population status of bream in several Moldovian Rivers and reservoirs were studied. Th...

  19. State of bream populations in reconstructed water bodies of Moldova

    Directory of Open Access Journals (Sweden)

    Adrian USATYI

    2010-11-01

    Full Text Available Bream, Abramis brama L., is a key species in fish communities of Moldovian Rivers and a main component of bottom food chains of river and lake ecosystems. With the reconstruction of water bodies, mainly for hydroelectric power stations needs, the ecological conditions changed substantially, which results in modifications in population state of biota. The negative effects of water bodies’ reconstruction upon population status of bream in several Moldovian Rivers and reservoirs were studied. These effects manifest in detrimental changes in bream growth, age composition and reproductive success. The conclusion is made that the economical gain after the regulation of large and middle-size rivers is opposed by the negative impact upon fish community as a whole, as well as upon the ecology of individual species as applied to the indicator species Abramis brama.

  20. Low cytochrome b variation in bream Abramis brama.

    Science.gov (United States)

    Hayden, B; Coscia, I; Mariani, S

    2011-05-01

    Variability in cytochrome b (cytb) in European populations of bream Abramis brama was assessed. The cytb gene was found to be strongly conserved in A. brama relative to other cyprinid taxa. This limits the usefulness of this marker in examining geographical genetic structure in this species and raises interesting questions as to the recent evolutionary history of the species. PMID:21539561

  1. PHYTOPLANKTON COMPOSITION IN FISH FARMS ALONG THE EASTERN ADRIATIC COAST

    OpenAIRE

    Marija Tomec

    2006-01-01

    Investigations of net phytoplankton composition were performed at three fish farms situated at the northern, middle and southern part of the eastern Adriatic Sea coast, respectively. In the northern part investigations were conducted in the Limski kanal, in the middle part at the Ugljan island and in the southern part in the place Drače on the Pelješac peninsula (Figure 1). At all three localities fish culture included mostly two species: gilthead sea bream (Sparus aurata) and sea bass (Dicen...

  2. 3D Morphology, Ultrastructure and Development of Ceratomyxa puntazzi Stages: First Insights into the Mechanisms of Motility and Budding in the Myxozoa

    Czech Academy of Sciences Publication Activity Database

    Alama-Bermejo, G.; Bron, J. E.; Raga, J. A.; Holzer, Astrid S.

    2012-01-01

    Roč. 7, č. 2 (2012), e32679. E-ISSN 1932-6203 Institutional support: RVO:60077344 Keywords : ELECTRON-MICROSCOPIC DESCRIPTION * BREAM SPARUS-AURATA * N-SP MYXOSPOREA * HOST-PARASITE RELATIONSHIP * DICENTRARCHUS-LABRAX L * CULTURED SEA BASS * TETRACAPSULOIDES-BRYOSALMONAE * DIPLODUS-PUNTAZZO * ENTERIC PARASITE * ACTIN DYNAMICS Subject RIV: GJ - Animal Vermins ; Diseases, Veterinary Medicine Impact factor: 3.730, year: 2012 http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0032679

  3. The effect of alpha-ketoglutarate on a piscine skin model: a molecular and morphological study

    OpenAIRE

    Costa, Rita

    2009-01-01

    Dissertação mest., Ciências Biomédicas, Universidade do Algarve, 2009 Alpha-ketoglutarate (AKG) is a key intermediate in the tricarboxylic acid cycle with important functions in glutamate and glutamine metabolism. Its effect after oral administration was studied in adult sea bream Sparus aurata skin and scales in order to evaluate the effect on collagen synthesis. Scales were removed from one side of the body and allowed to regenerate for 14 days; the control group received unt...

  4. Update on Fish Disease Situation in Italy

    DEFF Research Database (Denmark)

    Vendramin, Niccolò; Toffan, A.

    2012-01-01

    In this presentation we provide a general overview of the aquatic animal health issues related to the aquaculture sector and wild environment in Italy in 2011. Considering saltwater species European sea bass (Dicentrarchus labrax) and Gilthead sea bream (Sparus aurata) are still the most widely...... Amyloodinium ocellatum) and gill flukes (Diplectanum aequans and Sparicotyle chrysophrii) mainly present in inland farms (earth ponds and concrete tanks based); isopods crustacea (Ceratotoa, Anylocra) and Enteromyxidiosis (Enteromyxum leei) represent a treat for offshore cages. Among the dismetabolic unknown...

  5. Partition of aerobic and anaerobic swimming costs and their correlation to tail-beat frequency and burst activity in Sparus aurata

    DEFF Research Database (Denmark)

    Steffensen, John Fleng

    2012-01-01

    until fatigue at 10°C. The anaerobic swimming cost was measured as the excess postexercise oxygen consumption (EPOC) following each swimming speed. To determine tail-beat frequency, amplitude and burst and coast behaviour, the peduncle position was determined at 25 s·' by video tracking. The data showed...... EPOC and both the burst frequency (bursts min·') and burst distance (percentage burst distance) were found to predict EPOC by linear regressions. The low temperature used in the present study resulted in a prolonged recovery time, which increased with the anaerobic contribution to 10 hours after...

  6. The Role of stress coping style in reproduction and other biological aspects in the aquaculture species, Senegalese sole (Solea senegalensis) and gilthead seabream (Sparus aurata)

    OpenAIRE

    Ibarra Zatarain, Zohar; Balasch, Joan Carles

    2015-01-01

    Los individuos de una misma población consistentemente difieren en sus respuestas al estrés y comúnmente se reconocen como estilos afrontamiento al estrés (EAE). Los EAE se caracterizan por dos tipos comportamientos: proactivo y reactivo. Los peces proactivos se reconocen por su mayor actividad, son impulsivos en sus decisiones, se arriesgan más en situaciones de peligro y muestran bajos niveles de glucocorticoides, en comparación con los peces reactivos. Numerosos estudios han demostrado que...

  7. The role of stress coping style in reproduction and other biological aspects in the aquaculture species, Senegalese sole (Solea senegalensis) and gilthead seabream (Sparus aurata).

    OpenAIRE

    Ibarra Zatarain, Zohar

    2015-01-01

    Los individuos de una misma población consistentemente difieren en sus respuestas al estrés y comúnmente se reconocen como estilos afrontamiento al estrés (EAE). Los EAE se caracterizan por dos tipos comportamientos: proactivo y reactivo. Los peces proactivos se reconocen por su mayor actividad, son impulsivos en sus decisiones, se arriesgan más en situaciones de peligro y muestran bajos niveles de glucocorticoides, en comparación con los peces reactivos. Numerosos estudios han demostrado que...

  8. Effect of seaweed supplementation on growth performance, immune and oxidative stress responses in gilthead seabream (Sparus aurata)

    OpenAIRE

    Augusto Cesar dos Santos Queiroz; Rui Pereira; Andreia Ferreira Domingues

    2014-01-01

    Seaweeds have important nutraceutical properties, including antioxidant and biological response-modifying qualities. Their dietary supplementation may increase the immune and antioxidant capacity of fish necessary to cope to stressful conditions and minimizing disease outbreaks. There are essentially four major groups of seaweed that can be classified by color, namely green (Chlorophyta), brown/yellow (Phaeophyta), red (Rhodophyta), and blue-green (Cyanophyta). Some green seaweed has active ...

  9. Effectiveness of the anaesthetic MS-222 in gilthead seabream, Sparus aurata: effect of feeding time and day-night variations in plasma MS-222 concentration and GST activity.

    Science.gov (United States)

    Vera, L M; Montoya, A; Sánchez-Vázquez, F J

    2013-02-17

    Feeding time is a potent zeitgeber capable of synchronising behavioural and physiological daily rhythms in fish. However, the effect of feeding time on the daily rhythm of drugs toxicity and/or effectiveness remains unexplored to date. In this paper we investigated the day/night variations in the effectiveness of an anaesthetic commonly used in fish (Tricaine, MS-222) in a teleost of great chronobiological and aquaculture interest (gilthead seabream). To this end, fish were kept under LD 12:12 and fed at mid-light (ML), mid-darkness (MD) or random times (RD). The time needed to induce anaesthesia (reduction of locomotor activity) during MS-222 exposure (65 mg/L) as well as the recovery period were investigated at ML and MD in the three experimental groups using specialised video tracking software. In addition, daily rhythms of GST activity in the liver (as an indicator of detoxification processes) and plasma MS-222 concentration (related to uptake) were determined. The results revealed that MS-222 effectiveness in the ML group was higher during the day than at night (significant reduction of activity after 3 min vs. 5 min) whereas in the MD group, the daily variation of MS-222 effectiveness was inverted (significant reduction of activity after 7 min at ML vs. 2 min at MD), suggesting that feeding time can shift the day-night variations in the effectiveness of MS-222. Hepatic GST also seemed to be affected by feeding time: in fish fed at MD or RD this enzyme activity showed significant differences during the day, and the highest levels were found at different times of the day in each group. Plasma MS-222 concentrations were higher at ML (142.4±12.8 ng/ml) than at MD (96.3±10.9 ng/ml) (t-Student, peffectiveness of this anaesthetic. Furthermore, manipulation of the feeding schedule can be used to modify the daily variations in MS-222 effectiveness, which has basic as well applied implications for optimising anaesthesia protocols in fish aquaculture. PMID:23291569

  10. Impact of fish species on levels of lead accumulation in the meat of common bream (Abramis brama L.), white bream (Blicca bjoerkna L.) and common bleak (Alburnus alburnus L.) from the Vistula River (Poland)

    OpenAIRE

    Magdalena STANEK; Janusz DĄBROWSKI; Bogdan JANICKI; Aleksandra Roślewska; Anita Strzelecka

    2015-01-01

    The aim of this work was to compare the concentration of lead in the meat of common bream (Abramis brama L.), white bream (Blicca bjoerkna L.) and common bleak (Alburnus alburnus L.). The experimental fish were obtained in natural condition from Vistula River, located within Toru., near wastewater treatment plant. The study involved 60 individuals of freshwater fish caught in autumn. Analyses were carried out on 10 individuals of common bream, 20 white bream and 30 individuals of common bleak...

  11. Fish polar lipids retard atherosclerosis in rabbits by down-regulating PAF biosynthesis and up-regulating PAF catabolism

    Directory of Open Access Journals (Sweden)

    Nasopoulou Constantina

    2011-11-01

    Full Text Available Abstract Background Platelet activating factor (PAF has been proposed as a key factor and initial trigger in atherosclerosis. Recently, a modulation of PAF metabolism by bioactive food constituents has been suggested. In this study we investigated the effect of fish polar lipid consumption on PAF metabolism. Results The specific activities of four PAF metabolic enzymes; in leukocytes, platelets and plasma, and PAF concentration; either in blood cells or plasma were determined. Samples were acquired at the beginning and at the end of a previously conducted study in male New Zealand white rabbits that were fed for 45 days with atherogenic diet supplemented (group-B, n = 6 or not (group-A, n = 6 with gilthead sea bream (Sparus aurata polar lipids. The specific activity of PAF-Acetylhydrolase (PAF-AH; a catabolic enzyme of PAF, was decreased in rabbits' platelets of both A and B groups and in rabbits' leukocytes of group A (p 0.05. Free and bound PAF levels increased in group A while decreased in group B (p Conclusions Gilthead sea bream (Sparus aurata polar lipids modulate PAF metabolism upon atherosclerotic conditions in rabbits leading to lower PAF levels and activity in blood of rabbits with reduced early atherosclerotic lesions compared to control group.

  12. Variability of morphological characteristics of bream Abramis brama in water basins of upper Volga region

    OpenAIRE

    I. A. Stolbunov

    2005-01-01

    Data on variability of morphological readings of bream in the Rybinsk reservoir, and also in the system of reservoirs and water-currents (lake Galichskoe – river Kostroma – river Sot’ – the Kostroma expansion – Gorki reservoir) were generalized.

  13. First record of the common bream (Abramis brama) introduced to the Iberian Peninsula

    OpenAIRE

    Benejam Vidal, Lluís; Carol Bruguera, Joaquim; Alcaraz Cazorla, Carles; García-Berthou, Emili

    2005-01-01

    This is the first record of the common bream, Abramis brama (Linnaeus, 1758), introduced into the Iberian Peninsula. Eight individuals of this cyprinid fish species were captured (of a total of 978 fish) in the Boadella Reservoir (Catalonia, Spain) on August 18, 2004. This reservoir is only 14 km away from France, where the bream is native, and contains several exotic freshwater fish that are still not widespread in Spain. The further introduction of species and the illegal translocation of t...

  14. Morphometric indices of the bream (Abramis brama) in the Kremenchuk reservoir

    OpenAIRE

    D. S. Khrystenko

    2006-01-01

    The article considers the long-term resultant action of complex of existing factors on the morphometric indices of the bream (Abramis brama L.) in the Kremenchuk reservoir. The breams with the best exterior indices were fished by traditional fishing net during more than 40 years. This fact resulted in the survival of individuals with certain direction of variability and conduces to the change of an average index to particular value. As a result of the research, functional accordance of the mo...

  15. A survey on osmoregulatory potential of Bream, Abramis brama (Berg, 1949) fry for restocking management programs

    OpenAIRE

    Reza Asgari; Bagher Mojazi Amiri; Soheil Eagderi; Mohammad Ali Nematollahi

    2013-01-01

    Bream (Abramis brama, Berg, 1949; Family: Cyprinidae) is commercially valuable fish in the Caspian Sea fishing industry. Iranian Fisheries Organization annually produces and release up to 19 million Bream fries size for recruiting of this species. Its fries are mostly released into the Anzali wetland with 4 ppt salinity. Meanwhile, they sometimes are released into Sefidrood River (0.5 ppt), Sefidrood River estuary (8 ppt) and directly into the Caspian Sea (12 ppt). To determine whether these ...

  16. Effects of Starvation in Rock Bream, Oplegnathus fasciatus and Olive Flounder, Paralichthys olivaceus

    OpenAIRE

    Park, In-Seok; Gil, Hyun Woo; Yoo, Gwang Yeol; Oh, Ji Su

    2015-01-01

    We assessed the effects of various dietary conditions on the growth, phenotypic traits, and morphometric dimensions of rock bream, Oplegnathus fasciatus and on the morphometric dimensions of sectioned olive flounder, Paralichthys olivaceus. Rock bream in the fed group increased in body weight, standard length, and condition factor, but these parameters decreased significantly for fish in the starved group (P < 0.05). The head connection dimensions of fish in the fed group decreased, while for...

  17. Complete life cycle of Myxobolus rotundus (Myxosporea: Myxobolidae), a gill myxozoan of common bream Abramis brama.

    Science.gov (United States)

    Székely, Cs; Hallett, S L; Atkinson, S D; Molnár, K

    2009-06-10

    The life cycle of Myxobolus rotundus Nemeczek, 1911, a myxosporean parasite of the gills of common bream Abramis brama L., was studied under laboratory conditions. Mature Myxobolus spp. spores from plasmodia in the gills of wild bream were used to infect naïve oligochaete worms in a flow-through system of aquaria. Triactinomyxon-type actinospores were released from the oligochaetes 1 yr later and allowed to continually flow into a tank containing uninfected bream fry. The gills of the fry were checked for development of plasmodia in squash preparations 3 d postexposure, and then at weekly intervals for 8 wk. Tissue samples were fixed at each time point. Developing plasmodia were first observed 17 d post-exposure (Day 17). Mature spores were collected from plasmodia on Day 56 and were added to plastic dishes containing parasite-free Tubifex tubifex oligochaetes. Second-generation actinospores were released from these worms 8 mo post-exposure, and were morphologically identical to first-generation spores. Myxospores obtained from the bream fry were morphologically identical to those identified in wild bream as M. rotundus. Small subunit ribosomal RNA gene sequences obtained from first- and second-generation actinospores and the bream fry myxospores were 100% similar to M. rotundus spores from the original wild fish. PMID:19694174

  18. THE INFLUENCE OF FISHING ON THE AGE STRUCTURE OF BREAM (ABRAMIS BRAMA) OF THE DNIEPER-BUG ESTUARY REGION

    OpenAIRE

    U. Pilipеnko; І. Lobanov; V. Kornijenko

    2013-01-01

    The analysis of the dynamics of the age structure of the Lower Dnieper population of bream (Abramis brama) under increased pressure fishing is carried out. The data on the dynamics of indicators of the middle-weighted age of bream catches in the last ten years are determined.

  19. THE INFLUENCE OF FISHING ON THE AGE STRUCTURE OF BREAM (ABRAMIS BRAMA OF THE DNIEPER-BUG ESTUARY REGION

    Directory of Open Access Journals (Sweden)

    U. Pilipеnko

    2013-03-01

    Full Text Available The analysis of the dynamics of the age structure of the Lower Dnieper population of bream (Abramis brama under increased pressure fishing is carried out. The data on the dynamics of indicators of the middle-weighted age of bream catches in the last ten years are determined.

  20. Influence of fasting on muscle composition and antioxidant defenses of market-size Sparus macrocephalus *

    OpenAIRE

    Zhang, Xiao-dong; Wu, Tian-xing; Cai, Li-sheng; Zhu, Yong-fei

    2007-01-01

    The study was conducted to investigate fasting effects on flesh composition and antioxidant defenses of market-size Sparus macrocephalus. Two hundred fish (main initial weight 580 g) were divided into two groups (control and fasted) and reared in 6 cages. After two weeks of adaptation, group I fasted for 28 d; group II was fed normally as a control. In 3, 7, 14, 21 and 28 d, 6 fish per group were sampled for proximate flesh composition, liver antioxidant enzyme activities and malondialdehyde ...

  1. THE CONTENT OF SOME COMPONENTS OF METABOLISM IN MUSCLE TISSUE OF BREAM ABRAMIS BRAMA (L.

    Directory of Open Access Journals (Sweden)

    Ekaterina Flerova

    2014-02-01

    Full Text Available Physiological condition is one of the most important characteristic used for evaluating single individuals, populations and fish stocks in general. The data on a number of physiological indicators of bream the Gorky water reservoir were presented. Amount of moisture, dry matter in the muscular tissue was determined of using two-stage determination of total moisture. Simple lipids were determined by extraction with petroleum-ether. The analysis of the results suggests that the indices of water, dry matter and simple lipids content in muscular tissue of mature males, females and juvenile individuals of bream were close. Decrease of water amount in the muscle tissue, increase of dry matter end lipids takes place along with bream ageing.

  2. A survey on osmoregulatory potential of Bream, Abramis brama (Berg, 1949 fry for restocking management programs

    Directory of Open Access Journals (Sweden)

    Reza Asgari

    2013-03-01

    Full Text Available Bream (Abramis brama, Berg, 1949; Family: Cyprinidae is commercially valuable fish in the Caspian Sea fishing industry. Iranian Fisheries Organization annually produces and release up to 19 million Bream fries size for recruiting of this species. Its fries are mostly released into the Anzali wetland with 4 ppt salinity. Meanwhile, they sometimes are released into Sefidrood River (0.5 ppt, Sefidrood River estuary (8 ppt and directly into the Caspian Sea (12 ppt. To determine whether these alternative locations are suitable for release, sixty fingerling size Bream (0.5±0.13 g b.w. were exposed to four salinity levels of these locations i.e. 0.5, 4, 8 and 12 ppt, for 120 hrs. The results displayed that accumulated mortality rate was significantly lower in exposed fish to 4 ppt (PPP

  3. Influence of fasting on muscle composition and antioxidant defenses of market-size Sparus macrocephalus

    Institute of Scientific and Technical Information of China (English)

    2007-01-01

    The study was conducted to investigate fasting effects on flesh composition and antioxidant defenses of market-size Sparus macrocephalus. Two hundred fish (main initial weight 580 g) were divided into two groups (control and fasted) and reared in 6 cages. After two weeks of adaptation, group Ⅰ fasted for 28 d; group Ⅱ was fed normally as a control. In 3, 7, 14, 21 and 28 d,6 fish per group were sampled for proximate flesh composition, liver antioxidant enzyme activities and malondialdehyde flesh content analyses. In fasted fish, the reduction of lipid content in muscle occurred after day 3, and, compared to controls, the content of protein decreased from day 14, the activities of liver antioxidative enzymes superoxide dismutase (SOD) and glutathione peroxidase (GPX) increased from day 3, and flesh malondialdehyde levels increased from day 21. Flesh fat reduction shows that fasting may be used as a technique to reduce flesh lipid content in Sparus macrocephalus. However, considering flesh protein loss and the subsequent oxidative stress, the fasting technique should be used with precautions.

  4. Morphometric indices of the bream (Abramis brama in the Kremenchuk reservoir

    Directory of Open Access Journals (Sweden)

    D. S. Khrystenko

    2006-12-01

    Full Text Available The article considers the long-term resultant action of complex of existing factors on the morphometric indices of the bream (Abramis brama L. in the Kremenchuk reservoir. The breams with the best exterior indices were fished by traditional fishing net during more than 40 years. This fact resulted in the survival of individuals with certain direction of variability and conduces to the change of an average index to particular value. As a result of the research, functional accordance of the morphometric indices with the environmental conditions was ascertained.

  5. Impact of fish species on levels of lead accumulation in the meat of common bream (Abramis brama L., white bream (Blicca bjoerkna L. and common bleak (Alburnus alburnus L. from the Vistula River (Poland

    Directory of Open Access Journals (Sweden)

    Magdalena STANEK

    2015-06-01

    Full Text Available The aim of this work was to compare the concentration of lead in the meat of common bream (Abramis brama L., white bream (Blicca bjoerkna L. and common bleak (Alburnus alburnus L.. The experimental fish were obtained in natural condition from Vistula River, located within Toru., near wastewater treatment plant. The study involved 60 individuals of freshwater fish caught in autumn. Analyses were carried out on 10 individuals of common bream, 20 white bream and 30 individuals of common bleak. The muscles samples for analyses were taken from the large side muscle of fish body above the lateral line. There were chosen for analyses individuals with similar biometric measurements. Due to a relatively low amounts of meat obtained from white bream and common bleak, the material from individuals of similar body length was combined (about 2-3 pieces. Pb concentrations were determined by atomic absorption spectrophotometer Solaar 939 QZ, ATI Unicam. Analyses of variance (test post hoc -Tukey test indicated that the mean value of lead was the highest in the meat of common bream (0.086 ƒĘgEg-1 wet weight and the lowest in the meat of white bream (0.075 ƒĘgEg-1 wet weight. There were no statistical significant differences in the lead content between the analyzed fish species (at p< 0.05. Analysis of correlation indicated a negative and statistical significant correlation between the fish body length and Pb concentration.

  6. Variability of morphological characteristics of bream Abramis brama in water basins of upper Volga region

    Directory of Open Access Journals (Sweden)

    I. A. Stolbunov

    2005-09-01

    Full Text Available Data on variability of morphological readings of bream in the Rybinsk reservoir, and also in the system of reservoirs and water-currents (lake Galichskoe – river Kostroma – river Sot’ – the Kostroma expansion – Gorki reservoir were generalized.

  7. Comparative study on milt quality features of different finfish species

    Directory of Open Access Journals (Sweden)

    Rodolfo Ballestrazzi

    2010-01-01

    Full Text Available The aim of this research was to study the main sperm characteristics of three different finfish species. Twenty-one gilt-  head sea bream (Sparus aurata, 20 brown trout (Salmo trutta, morpha fario and 15 rainbow trout (Oncorhynchus  mykiss male broodstocks, farmed under optimal conditions for each species and fed standard diets for broodstocks, were  manually stripped. Brown trout yielded small amounts of sperm (4.5 vs18.13 ml that were very concentrated (≅8.5 x  109 vs 1.24 x 109 Szoa/ml with respect to the other species. The duration of spermatozoan motility for gilthead sea  bream sperm was significantly longer (almost 50 min, in comparison to the one-minute motility of Salmonids. Single  fatty acids of brown trout sperm were higher than in the other two species for almost all detected fatty acids. In partic-  ular, eicosapentaenoic acid (EPA was at least three times more concentrated in brown trout than in rainbow trout or gilt-  head sea bream sperm (1238.3 µg/g vs305.6 and 333.3 µg/g, respectively; P   total unsaturated fatty acid classes were significantly higher in brown trout sperm than in the other two species - almost  double with respect to gilthead sea bream sperm and more than double in comparison to RT sperm (P  

  8. DIETS IN SEA BASS AND SEA BREAM REARING — DEVELOPMENT AND PROSPECTIVE

    Directory of Open Access Journals (Sweden)

    Lav Bavčević

    2006-07-01

    Full Text Available Sea bass feeding, in the early beginning of cage culture activity, was based on modified trout pellets and minced sardines. Soon–after knowledge on sea bass and sea bream nutritional needs has been rapidly improved. Recommended balance between digestible proteins and digestible energy (DP/DE in sea bass and sea bream diets according to different authors is between 19— 21 MJ/kg and 21–24 MJ/kg. Variations of optimal DP/DE in scientific publications in the past, seems to be reflected on significant variation of DP/DE in commercial diets of different producers. Simple method to estimate optimal amino acid balance in the diet is amino acid analysis of whole body protein. Fat content in feed for sea bass and sea bream is around 20 %. Required enrichment with essential fatty acids (especially HUFA n 3 is usually resolved when at least 7% of fish oil is added in the diet. Essential fatty acid (EFA relative content in farmed sea bass and sea bream muscle fat was found to be similar to the relative content of EFA in fish oil. Vitamins–mineral premixes are standardized and given in higher concentration only in special feeds which can be enriched with immunostimulatns, and used in specific circumstances to improve immunobalance. To establish new feed production for sea bass and sea bream rearing, beside needed knowledge of nutritive value of raw materials and seasonal variations in fish demand, is important to know growth dynamics of cultured organisms and, is the most important — market situation. Competition and increased problems with quality raw material supply are constantly increasing production cost effective value. Today the margin for income–outcome equilibrium are 20 000 t, with the accent that it will be higher in coming years.

  9. Toxicity of dispersant application: Biomarkers responses in gills of juvenile golden grey mullet (Liza aurata).

    Science.gov (United States)

    Milinkovitch, Thomas; Godefroy, Joachim; Théron, Michaël; Thomas-Guyon, Hélène

    2011-10-01

    Dispersant use in nearshore areas is likely to increase the exposure of aquatic organisms to petroleum. To measure the toxicity of this controversial response technique, golden grey mullets (Liza aurata) were exposed to mechanically dispersed oil, chemically dispersed oil, dispersant alone in seawater, water-soluble fraction of oil and to seawater as a control treatment. Several biomarkers were assessed in the gills (enzymatic antioxidant activities, glutathione content, lipid peroxidation) and in the gallbladder (polycylic aromatic hydrocarbons metabolites). The significant differences between chemically dispersed oil and water soluble fraction of oil highlight the environmental risk to disperse an oil slick when containment and recovery can be conducted. The lack of significance between chemically and mechanically dispersed oil suggests that dispersant application is no more toxic than the natural dispersion of the oil slick. The results of this study are of interest in order to establish dispersant use policies in nearshore areas. PMID:21592637

  10. Comparison of genetic variation of wild and farmed Bream (Abramis brama orientalis; berg, 1905) using microsatellite markers

    OpenAIRE

    Zeinab Hosseinnia; Ali Shabany; Hamed Kolangi-Miandare

    2014-01-01

    Bream (Abramis brama orientalis) is one of the most commercially valuable fish in the Caspian Sea. The aim of this study was to compare levels of genetic polymorphism between wild and farmed Bream populations using seven microsatellite loci. Genetic diversity was investigated by studying samples collected from two regions; Chaboksar and the Artificial Propagation Center of Guilan province. Allele frequency was found to have declined in wild and cultured fish due to inbreeding and genetic drif...

  11. The effect of artificial feed on blood biochemistry profile and liver histology of wild saddled bream, Oblada melanura (Sparidae)

    OpenAIRE

    FERRI, Josipa; Popović, Natalija Topić; Čož-Rakovac, Rozelinda; Beer-Ljubić, Blanka; Strunjak-Perović, Ivančica; Škeljo, Frane; Jadan, Margita; Petrić, Mirela; Barišić, Josip; Šimpraga, Miljenko; Stanić, Rino

    2011-01-01

    Abstract Floating fish farms attract a great number of wild fish species, changing their behaviour and physiology. The saddled bream, Oblada melanura, sampled from populations aggregated around the Adriatic fish farm and from natural/control populations, were analysed for differences in eleven blood biochemistry parameters and liver histomorphology. The levels of lactate dehydrogenase (LDH), alkaline phosphatase (ALKP) and urea (URE) in cage-associated saddled bream (428.00 ? SD 32...

  12. The role of freshwater habitats for the reproduction of common bream Abramis brama (L.) in a brackish water system

    Science.gov (United States)

    Kafemann, R.; Thiel, R.; Finn, J.E.; Neukamm, R.

    1998-01-01

    Abundance and biomass data for juveniles and adults, length frequency histograms and the electron microprobe analysis (EPMA) of otoliths were used to indicate density, migration and reproduction of common bream Abramis brama in the Kiel Canal drainage, Germany. The reproduction of common bream was primarily restricted to two types of spawning habitats: one in the Haaler Au, a freshwater tributary and another in shallow, oligohaline portion of the main Canal. Both spawning habitats were morphologically characterized as shallow with submerged vegetation. During April to June concentrations of spawners were observed, whereas age-0 common bream dominated from August through December. The distribution of age-0 common bream was primarily restricted to fresh and oligohaline waters. Outside the spawning season, the distribution of common bream was less obvious. Adult fish were more widely distributed within the Canal, indicating a tolerance for higher salinities. During the spawning season common bream seem to show an exceptional mobility between spawning and feeding habitats, which are denoted by different salinities.

  13. Induced artificial androgenesis in common tench, Tinca tinca (L., using common carp and common bream eggs

    Directory of Open Access Journals (Sweden)

    Dariusz Kucharczyk

    2014-03-01

    Full Text Available This study presents artificial induction using tench eggs, Tinca tinca (L., of androgenetic origin. The oocytes taken from common bream, Abramis brama (L. and common carp, Cyprinus carpio L. were genetically inactivated using UV irradiation and then inseminated using tench spermatozoa. Androgenetic origin (haploid or diploid embryos was checked using a recessive colour (blond and morphological markers. The percentage of hatched embryos in all experimental groups was much lower than in the control groups. All haploid embryos showed morphological abnormalities, which were recorded as haploid syndrome (stunted body, poorly formed retina, etc.. The optimal dose of UV irradiation of common bream and common carp eggs was 3456 J m–2. At this dose, almost 100% of haploid embryos were produced at a hatching rate of over 6%. Lower UV-ray doses affected abnormal embryo development. The highest yield of tench androgenesis (about 2% was noted when eggs were exposed to thermal shock 30 min after egg activation.

  14. Dietary keto-acid feed-back on pituitary activity in gilthead sea bream

    DEFF Research Database (Denmark)

    Ibarz, Antoni; Costa, Rita; Harrison, Adrian Paul;

    2010-01-01

    The influence of a daily oral dose of alpha-ketoglutarate (AKG, 0.1 g/kg body weight), an intermediate metabolite in the Krebs cycle and a dietary additive, on the pituitary proteome of gilthead sea bream was determined by two-dimensional electrophoresis (2-DE). A high-resolution map of the sea...... bream pituitary proteome was generated. Proteins with a modified expression between Controls and AKG treated fish were further analysed by MALDI-TOF/TOF-MS and liquid chromatography combined with a nanoelectrospray (LC-MS/MS). The main changes in the proteome induced by AKG treatment were grouped....... Metabolic proteins up-regulated with AKG supplementation included fructose-bis-phosphate aldolase, glyceraldehyde-phosphate dehydrogenase and malate dehydrogenase, all related to glucose metabolism (p...

  15. THE CONTENT OF SOME COMPONENTS OF METABOLISM IN MUSCLE TISSUE OF BREAM ABRAMIS BRAMA (L.)

    OpenAIRE

    Ekaterina Flerova; Aleksandra Kostyleva

    2014-01-01

    Physiological condition is one of the most important characteristic used for evaluating single individuals, populations and fish stocks in general. The data on a number of physiological indicators of bream the Gorky water reservoir were presented. Amount of moisture, dry matter in the muscular tissue was determined of using two-stage determination of total moisture. Simple lipids were determined by extraction with petroleum-ether. The analysis of the results suggests that the indices of water...

  16. Detection of Range Finding Test of Mercury Chloride in Yellowfin Sea Bream (Acanthopagrus latus)

    OpenAIRE

    Aliakbar Hedayati; Alireza Safahieh; Ahmad Savari; Jasem Ghofle Marammazi

    2010-01-01

    Toxicity tests allow the determination of pollution effects, providing direct evidence of the biological responses of marine organisms to contaminants. Fifty four yellow fine sea bream all immature male in same size (120 g final body weight average) were obtained from Mahshahr creeks with hooks in a Upon capture. In laboratory Fish maintained in a seawater re-circulatory system (300-L tanks) equipped with physical/biological filters and with aeration. All samples were acclimated for one weeks...

  17. PIXE analysis of otoliths from reared red sea bream, pagrus major (temminck et schlegl)

    International Nuclear Information System (INIS)

    PIXE analysis was applied to estimate mineral concentration in red sea bream otoliths without cutting or polishing. Detected elements include Sr, Fe, Mn and Zn, which are commonly found in the otoliths in marine fishes. Strontium-Calcium concentration ratio is calculated by means of combined X-ray yields. The ratio doesn't indicate clear correlaion with mean reared seawater temperature. It is caused by the diffraction error induced by rough topographies of the otoliths surface. (author)

  18. Beaufort Region Environmental Assessment and Monitoring program (BREAM). Final report for 1992/1993

    International Nuclear Information System (INIS)

    The Beaufort Region Environmental Assessment and Monitoring (BREAM) program was established to identify environmental research and monitoring priorities related to future hydrocarbon development activities in the Beaufort Sea and Mackenzie Delta region. The activities occurring during the third year of BREAM focused on major oil spills. Three planning meetings were held: a Project Initiation Meeting and technical meetings of the Community-Based Concerns and Catastrophic Oil Spill Working Groups. The initiation meeting had goals that included identifying specific tasks to be completed by the two Working Groups, discussion of contents and scope of materials being prepared for an oil spill workshop, and determining project schedules. The Community-Based Concerns group focused its work on identifying ecological concerns related to oil spills and their cleanup, identifying community-based ecological issues and concerns, and incorporating local and traditional knowledge into the BREAM program. The group suggested changes to the wording of existing impact hypotheses and oil spill scenarios, and recommended changes in a list of valued ecosystem components. The oil spill group reviewed ecological concerns related to oil spills, and reviewed each oil spill scenario and impact hypothesis selected for an interdisciplinary workshop held in February 1993. The workshop evaluated four of the most important oil spill impact hypotheses (offshore platform blowout, river barge spill of diesel fuel, under-ice spill from a pipeline river crossing, and a pipeline spill affecting mammals). Further research and monitoring related to a number of impact hypotheses was recommended by workshop participants. 57 refs., 29 figs., 12 tabs

  19. Molecular Cloning and Sequence Analysis of IGF-I from Triangular Bream(Megalobrama terminalis)

    Institute of Scientific and Technical Information of China (English)

    TONG Fu-dan; LIU Hong-yun

    2004-01-01

    The insulin-like growth factor Ⅰ(IGF-Ⅰ)gene of triangular bream(Megalobrama terminalis)(GenBank No.AY247412)(Tb)was cloned for the first time from liver by RT-PCR. The nucleotide sequence analysis showed the Tb IGF-Ⅰ cDNA consisted of 486 nucleotides and encoded 117 amino acids including B,C,A,D and E five domains. Analysis of E-domain indicated that cloned Tb IGF-Ⅰ belonged to IGF-Ⅰ Ea-2 subtype. Identity analysis showed the IGF-Ⅰ nucleotide sequence shared 99.8% homology with bluntnose bream,88.8% with grass Carp,85.8% with common carp; the pre-IGF-Ⅰ amine acid sequence shared 99.4% with bluntnose bream,88.8% with grass carp,85.4% homology with common carp. In the Cyprinus Carpio,the higher homology of nucleotide sequence and amino acid sequence in IGF-Ⅰshowed that the closer relationship the fishes have. These results could provide basic data for the research on Tb germplasm and the development and utilization of biological feed additives.

  20. FAST Real Time PCR for control of intra-species recycling in aquaculture feed, focused to the most relevant fish species farmed in Europe.

    Science.gov (United States)

    Espiñeira, Montserrat; Vieites, Juan M

    2016-08-01

    Recent regulations in animal feed composition prohibit intra-species recycling, the recycling of one given animal species to the same species, in order to avoid potential safety risks to human and animal health. These regulations have generated the need of their control in aquaculture by effective and specific analytical techniques. To date, most studies of species identification and detection in feedstuffs are focused on land species, but few studies are focused on species composition in fish feed. The present work describes five methodologies based in Real Time PCR for detection of the most relevant fish species farmed in Europe: gilthead sea bream (Sparus aurata); sea bass (Dicentrarchus labrax); turbot (Scophthalmus maximus); rainbow trout (Onchorynchus mykiss); and salmon (Salmo salar), in order to guarantee the intra-species recycling regulation in aquaculture feedstuffs. PMID:26988512

  1. Duplicación, organización genómica y regulación transcripcional de los receptores de la hormona del crecimiento de peces. Aspectos básicos y aplicados en dorada Sparus aurata.

    OpenAIRE

    Saera Vila, Alfonso

    2009-01-01

    Los primeros receptores de hormona de crecimiento (GHR) descritos en peces se agruparon en dos grupos, las secuencias descritas en peces salmónidos y las descritas en peces no salmónidos. Las de peces no salmónidos presentaron una gran conservación estructural con las secuencias de mamíferos, pero las secuencias de peces salmónidos muestraron un bajo nivel de identidad aminoacídica con ausencia de un par de cisteínas extracelulares y un diferente patrón de tirosinas intracelulares. Llegados a...

  2. Efecto de la contaminación de la contaminación por nanopartículas metálicas en larvas de dorada (Sparus aurata). Estudio comparativo con otras especies

    OpenAIRE

    Mario Jesús Rosado Rodríguez

    2016-01-01

    Actualmente la contaminación por metales pesados y metaloides en medio acuático ha resultado ser uno de los mayores problemas ambientales, a pesar de que suelen encontrarse a niveles traza en el medio ambiente. Durante la última década, se ha investigado la presencia de muchos de estos elementos metálicos en medio acuático y sus efectos en los organismos debido a su alta toxicidad, persistencia y acumulación en los tejidos. Para los organismos vivos la acumulación de metales es tóxica, y por ...

  3. Caracterización de las colinesterasas presentes en cerebro y músculo de dorada (sparus aurata) y estudio de su inhibición por insecticidas organofosforados

    OpenAIRE

    Albendín García, Gemma

    2009-01-01

    Las colinesterasas se han venido utilizando como biomarcadores específicos para diagnosticar la exposición de poblaciones naturales a plaguicidas organofosforados y carbamatos. Mientras que los residuos de estos compuestos en el agua y en los tejidos de los peces generalmente desaparecen al cabo de varios días, la actividad colinesterasa en éstos permanece inhibida durante varias semanas. En relación con el ambiente acuático, a pesar de que la contaminación por este tipo de sustancias c...

  4. Variation in heavy metals concentration in the edible oyster Crassostrea madrasensis, clam Polymesoda erosa and grey mullet Liza aurata from coastline of India

    Digital Repository Service at National Institute of Oceanography (India)

    Gawade, L.; Chari, N.V.H.; Sarma, V.V.; Ingole, B

    . Variation in heavy metals concentration in the edible oyster Crassostrea madrasensis, clam Polymesoda erosa and grey mullet Liza aurata from coastline of India, Indian Journal of Science, 2013, 2(4), 59-63, www.discovery.org.in http://www.discovery.org.in... RESEARCH Gawade Lata et al. Variation in heavy metals concentration in the edible oyster Crassostrea madrasensis, clam Polymesoda erosa and grey mullet Liza aurata from coastline of India, Indian Journal of Science, 2013, 2(4), 59-63, www.discovery.org.in...

  5. Efecto del 17a-etinilestradiol sobre el sistema inmunitario y reproductor de la dorada (Sparus aurata L.). Caracterización funcional del receptor de estrógenos asociado a proteína G = Effect of 17a-ethinylestradiol on inmune and reproductive system of the gilthead seabream (Sparus aurata L.). Functional characterization of the G protein-couplet estrogen receptor

    OpenAIRE

    Cabas Sánchez, Isabel

    2013-01-01

    OBJETIVOS: 1. Evaluar la capacidad del EE2 de provocar una respuesta estrogénica en vivo en ejemplares macho de dorada 2. Evaluar la capacidad del EE2 de alterar la fisiología y la respuesta inmunitaria local de la gónada 3. Evaluar la habilidad del EE2 de alterar la respuesta inmunitaria sistémica, analizando actividades inmunitarias de leucocitos de riñón cefálico, el perfil de expresión de macrófagos y la capacidad en vivo de responder a un reto inmunológico. 4. Realizar una ...

  6. Risk assessment of the total mercury in Golden gray mullet (Liza aurata from Caspian Sea

    Directory of Open Access Journals (Sweden)

    Seyed Mehdi Hosseini

    2013-12-01

    Full Text Available Mercury is the most toxic heavy metal in the aquatic ecosystems which originates both from natural and industrial resources and is ultimately deposited in sediments as methyl mercury. This metal is quickly transferred through the food chain and accumulated in organisms. In this study, the human health risk due to consumption of Mullet (Liza aurata in the Caspian Sea, were evaluated by measuring the concentration of mercury in muscle samples using Atomic absorbtion spectrophotometer (Perkin Elmer FIAS-100 and cold vapor technique. A total of 60 fresh Mullet samples were collected by local fisherman from 12 stations on the southern coast of Caspian Sea in Mazandaran province situated in the north of Iran. The average concentration of mercury in Mullet muscle was 0.137 µg/g of fresh weight (0.432 µg/g dry weight which was less than the allowable amount for human consumption determined by the international organizations such as United States Environmental Protection Agency, World Health Organization, Food and Agriculture Organizations and the Food and Drug Administration. The calculations indicated that daily and weekly mercury uptake for Iranian consumers is lower than the guide values (Acceptable Daily Intake and Provisional Tolerable Weekly Intake provided by international organizations. Also, Hazard Quotient Index was below 1 (0.35. Therefore, the consumption of the Mullet is not a serious threat to the consumer’s health and a consumption permitted rate of 51 g per day is recommended.

  7. Toxicity of dispersant application: Biomarkers responses in gills of juvenile golden grey mullet (Liza aurata)

    International Nuclear Information System (INIS)

    Dispersant use in nearshore areas is likely to increase the exposure of aquatic organisms to petroleum. To measure the toxicity of this controversial response technique, golden grey mullets (Liza aurata) were exposed to mechanically dispersed oil, chemically dispersed oil, dispersant alone in seawater, water-soluble fraction of oil and to seawater as a control treatment. Several biomarkers were assessed in the gills (enzymatic antioxidant activities, glutathione content, lipid peroxidation) and in the gallbladder (polycylic aromatic hydrocarbons metabolites). The significant differences between chemically dispersed oil and water soluble fraction of oil highlight the environmental risk to disperse an oil slick when containment and recovery can be conducted. The lack of significance between chemically and mechanically dispersed oil suggests that dispersant application is no more toxic than the natural dispersion of the oil slick. The results of this study are of interest in order to establish dispersant use policies in nearshore areas. - Highlights: → This study simulates and evaluates the toxicity of dispersant use in nearshore area. → Dispersant use toxicity is assessed through biomarkers measurement in a fish species. → Chemical dispersion of an oil slick increases the petroleum toxicity. → Dispersant use does not enhance the toxicity of a mechanically dispersed oil slick. → This work leads to conclusions concerning dispersant use policies in nearshore area. - When the meteorological conditions induce the dispersion of the oil slick (e.g. wave), the application of dispersant does not increase the toxicity of petroleum.

  8. Effect of artificial regulations of Artemia n-3 HUFA content on growth and survival of black seabream ( Sparus macrocephalus) larvae

    Science.gov (United States)

    Liu, Jing-Ke

    1998-06-01

    The requirement for dietary n-3 HUFA (highly unsaturated fatty acid) for growth and survival of black seabream ( Sparus macrocephalus) larvae was tested using Artemia with various levels of n-3 HUFA. Four treatments with Artemia differing in their n-3 HUFA were prepared by feeding them various oil emulsions. The results indicated that dietary n-3 HUFA significantly influences fish n-3 HUFA levels and are necessary for good growth and survival of black seabream larvae. The proper value of n-3 HUFA level and DHA, EPA level in Artemia should be 4.273% and 0.873% (wet weight) for good growth and survival in black seabream larvae respectively.

  9. [Influence of development pace on pharyngeal teeth formula in Abramis brama (L.) bream: experimental data].

    Science.gov (United States)

    Bolotovskii, A A; Levin, B A

    2011-01-01

    An experiment on acceleration and retardation of ontogenesis with thyroid manipulation has revealed direct changes in definitive dentition of pharyngeal bones in Abramis brama bream. As development pace accelerates, the number of teeth reduces to the formula 5-4. When development pace slows down, this number increases to the formula 6-5. Moreover, an additional minor row of teeth (1.6-5.1, 2.6-5.2) is formed. The observed changes transcend typical changes happening in nature. It is assumed that heterochronies provoke changes in the number of teeth. PMID:21786649

  10. Mechanism of hybridization between bream Abramis brama and roach Rutilus rutilus in their native range.

    Science.gov (United States)

    Kuparinen, A; Vinni, M; Teacher, A G F; Kähkönen, K; Merilä, J

    2014-01-01

    Mechanisms of hybridization between bream Abramis brama and roach Rutilus rutilus were studied within the native range of the species in a lake in southern Finland. Through the genetic analysis of A. brama, R. rutilus and putative hybrids, hybridization is shown to have occurred between female A. brama and male R. rutilus. These results match with previous findings from introduced habitats, suggesting that mating between female A. brama and male R. rutilus is the predominant mechanism through which the two species hybridize. PMID:24383808

  11. Aspects on respiratory physiology of cultured Sea bream, Sparidentex hasta (Valenciennes 1830, Kingdom of Bahrain

    Directory of Open Access Journals (Sweden)

    Khadija Zainal

    2016-06-01

    The fish regulatory ability to withstand declining oxygen concentration in the water was limited. A typical steep straight line relationship was found between oxygen consumption rate and oxygen concentration indicating a non regulatory ability and extreme in-tolerance to hypoxia. Therefore, the fish is considered as oxy-conformer, i.e., unable to continue metabolism at anaerobic condition. Correlation between minimum (basic oxygen consumption rate and body weight was of non-linear form. The present study provides comparative data to base on for further prospective related studies on juvenile Sea bream and other fish species.

  12. Effect of dietary taurine and cystine on growth performance of juvenile red sea bream Pagrus major

    OpenAIRE

    Matsunari, Hiroyuki; Furuita, Hirofumi; Yamamoto, Takeshi; Kim, Shin-Kwon; Sakakura, Yoshitaka; Takeuchi, Toshio

    2008-01-01

    Two experiments were conducted to investigate the effect of dietary taurine and cystine on growth and body composition of juvenile red sea bream Pagrus major. In Experiment I, a casein-based semi-purified diet included a small amount of fish meal were supplemented with taurine at the levels of 0 (control) and 1.0%. The experimental diets in Experiment II were without fishmeal and supplemented with taurine at 0 (control), 0.5, 1.0 and 2.0% or cystine at 1.0 and 2.0%. These diets were fed three...

  13. Genetic diversity of Liza aurata (Risso, 1810 in the coastal regions of Golstan province, using microsatellite marker

    Directory of Open Access Journals (Sweden)

    Zohreh Ghodsi

    2011-06-01

    Full Text Available Golden grey mullet (Liza aurata is a commercially valuable fish with great demand due to its delicious taste in southern coastal parts of the Caspian Sea. Genetic diversity of marine resources is of vital importance in their management and protection, as this is the first prerequisite for maintaining the consistency of populations in an inconsistent environmental conditions. In this study, we have used six microsatellite locations to investigate the level of genetic variation of Liza aurata in Gomishan and Miyankale regions in Golestan province. The results showed no conspicuous genetic variations in these two regions using Fst, Rst and AMOVA and accordingly a relatively high level of gene flow was found among the populations. Genetic variations in Gomishan (mean number of alleles per locus, Na=14.667, mean effective number of alleles, Ne=10.355, observed heterozygosity, Ho=0.905 and expected heterozygosity, He=0.894 and Miyankale (Na=15, Ne=10.223, Ho=0.863 and He=0.892 were not statistically different. There were evidences for genetic bottleneck in the populations. Protection and restoration of habitats can help to increase the population size and decrease risk of vulnerability of the species in the future.

  14. [Effect of food availability in early ontogenesis on the rate of growth and numbers of bream Abramis brama L. (Cypriniformes, Cyprinidae) in Kursh Bay of the Baltic Sea].

    Science.gov (United States)

    Naumenko, E N

    2011-01-01

    On the basis of studies of zooplankton and ichthyoplankton in the coastal zone of Kursh Bay of the Baltic Sea, zooplankton production accessible for juveniles of bream Abramis brama L. at early stages of ontogenesis, degree of removal of food resources, and food availability of juveniles of the bream that was reflected in the rate of growth were calculated. It is concluded that the removal of more than 60% of production of food zooplankton by bream juveniles leads to an increase of their mortality at early stages and a decrease in the rate of increase in the body weight. PMID:21786657

  15. Observation of reproductive behaviour of the common bream .i.Abramis brama./i. by the scientific echosounder

    Czech Academy of Sciences Publication Activity Database

    Prchalová, M.; Kubečka, Jan

    Praha: ICARIS, 2002. s. 275. [International Conference on Reservoir Limnology and Water Quality /4./. 12.08.2002-16.08.2002, České Budějovice] Institutional research plan: CEZ:AV0Z6017912 Keywords : reproductive behaviour * common bream Subject RIV: EH - Ecology, Behaviour

  16. Relationship between liver PCB content and biochemical and morphometric measures in bream (Abramis brama) from Rybinsk Reservoir

    Energy Technology Data Exchange (ETDEWEB)

    Tillitt, D.E.; Zajicek, J.L. [NBS, Columbia, MO (United States); Chuiko, G.M.; Flerov, B.A.; Stepanova, V.M.; Zhelnin, Y.Y.; Pod`gornaya, V.A. [IBIW, Borok (Russian Federation)

    1995-12-31

    PCBs are widely distributed in aquatic environments throughout the world, due to local contamination and global transport. However, little is known of the local distribution of PCBs in Rybinsk Reservoir, Russia. The Rybinsk Reservoir, constructed in the 1940`s, is the largest artificial waterbody in Europe (4,550 km{sup 2}) and provides drinking water for cities in the surrounding area. Industrialization in Cherepovetz, a city at the northeastern portion of the reservoir including the largest metallurgical facility in Europe, has resulted in chemical contamination of the reservoir. The objective of this study was to investigate the extent of PCB contamination in Bream taken from six locations in the reservoir and to compare the chemical analyses with biochemical and morphometric indicators of fish health. Concentrations of PCBs ranged from non-detected to 3.4 ug/g of liver, with the greatest concentrations found in fish taken near the industrialized area. The pattern of the PCB congeners in the livers of Bream taken near Cherepovetz closely resembled that of the standard Aroclor 1254. PCB content was negatively correlated with protein content and acetylcholinesterase (AChE) activity in Bream liver. Other morphometric factors and biochemical measures varied among locations, but were unrelated to concentrations of PCBs in the livers of Bream.

  17. The analysis of the pharyngeal-sieve mechanism and the efficiency of food intake in the bream (Abramis brama, Cyprinidae).

    NARCIS (Netherlands)

    Hoogenboezem, W.

    1991-01-01

    This thesis describes the mechanism and the selectivity of food intake in bream ( Abramis brama ). It is a compilation of six articles which have been published (or will soon be published) in international journals.In the first chapter, diets and feeding modes in cyprinid fishes are described

  18. Threonine affects digestion capacity and hepatopancreatic gene expression of juvenile blunt snout bream (Megalobrama amblycephala).

    Science.gov (United States)

    Habte-Tsion, Habte-Michael; Ren, Mingchun; Liu, Bo; Xie, Jun; Ge, Xianping; Chen, Ruli; Zhou, Qunlan; Pan, Liangkun

    2015-08-28

    The present study conducted a 9-week feeding trial to investigate the effects of threonine (Thr) on the digestion capacity and hepatopancreas gene expression of juvenile blunt snout bream (Megalobrama amblycephala). For this purpose, three tanks (300 litres/tank) were randomly arranged and assigned to each experimental diet. Juvenile fish were fed with diets containing graded Thr levels (0·58, 1·08, 1·58, 2·08 or 2·58 % of the diet) to apparent satiation four times daily. At the end of the feeding trial, the results indicated that hepatopancreas weight, hepatosomatic index, hepatopancreatic protein content, intestinal weight, intestosomatic index and intestinal protein content increased with increasing dietary Thr levels up to 1·58 % and thereafter decreased (Pamylase and lipase elevated as dietary Thr levels increased up to 1·58 % (Pamylase, lipase, target of rapamycin and insulin-like growth factor-I were up-regulated, and the highest values were observed with 1·58 % dietary Thr or 1·58 and 2·08 % dietary Thr, whereas the relative gene expression levels of eukaryotic translation initiation factor 4E-binding protein 2 gradually decreased (P< 0·10) as dietary Thr levels increased up to 1·58 % and thereafter significantly increased (P< 0·05), which could explain that about 1·58 % dietary Thr could improve the growth and development of digestive organs and activities of digestive enzymes of juvenile blunt snout bream. PMID:26202077

  19. Molecular characterisation and biological activity of a novel CXC chemokine gene in rock bream (Oplegnathus fasciatus).

    Science.gov (United States)

    Kim, Ju-Won; Kim, Eun-Gyeong; Kim, Do-Hyung; Shim, Sang Hee; Park, Chan-Il

    2013-05-01

    Chemokines are chemoattractant cytokines defined by the presence of four conserved cysteine residues. In mammals, these cytokines can be divided into four subfamilies depending on the arrangement of the first two conserved cysteines in the sequence, and include the CXC(α), CC(β), C(γ), and CX3C(δ) classes. We identified CXC chemokine cDNA, designated RbCXC, isolated using expressed sequence tag analysis of a lipopolysaccharide (LPS)-stimulated rock bream liver cDNA library. The full-length RbCXC cDNA (742 bp) contained an open reading frame of 342 bp encoding 114 amino acids. Results from phylogenetic analysis showed that RbCXC was strictly separated into a distinct clade compared to other known CXC chemokine subgroups. RbCXC was significantly expressed in the trunk kidney, liver, spleen, gill, peripheral blood leukocytes (PBLs), and head kidney. Rock bream PBLs were stimulated with several mitogens, including LPS and polyinosinic-polycytidylic acid (poly I:C), which significantly induced the expression of RbCXC mRNA. RbCXC mRNA expression was examined in several tissues under conditions of bacterial and viral challenge. Experimental challenges revealed that all examined tissues from fish infected with Edwardsiella tarda and red sea bream iridovirus showed significant increases in RbCXC expression compared to the control. In the case of Streptococcus iniae infection, RbCXC mRNA expression was markedly upregulated in the kidney, spleen, and liver. In addition, a maltose binding protein fusion recombinant RbCXC (~53 kDa) was produced in an Escherichia coli expression system and purified. Subsequently, the addition of purified recombinant RbCXC (rRbCXC) to kidney leukocytes was examined to investigate the impact of proliferative and chemotactic activity. The rRbCXC induced significant kidney leukocyte proliferation and attraction at concentrations ranging from 10 to 300 μg/mL, suggesting that it can be utilised as an immune stimulant and/or molecular adjuvant to

  20. Coordinated gene expression during gilthead sea bream skeletogenesis and its disruption by nutritional hypervitaminosis A

    Directory of Open Access Journals (Sweden)

    Zambonino-Infante Jose

    2011-02-01

    Full Text Available Abstract Background Vitamin A (VA has a key role in vertebrate morphogenesis, determining body patterning and growth through the control of cell proliferation and differentiation processes. VA regulates primary molecular pathways of those processes by the binding of its active metabolite (retinoic acid to two types of specific nuclear receptors: retinoic acid receptors (RARs and retinoid X receptors (RXRs, which promote transcription of downstream target genes. This process is well known in most of higher vertebrates; however, scarce information is available regarding fishes. Therefore, in order to gain further knowledge of fish larval development and its disruption by nutritional VA imbalance, the relative expression of some RARs and RXRs, as well as several genes involved in morpho- and skeletogenesis such as peroxisome proliferator-activated receptors (PPARA, PPARB and PPARG; retinol-binding protein (RBP; insulin-like growth factors I and II (IGF1 and IGF2, respectively; bone morphogenetic protein 2 (Bmp2; transforming growth factor β-1 (TGFB1; and genes encoding different extracellular matrix (ECM proteins such as matrix Gla protein (mgp, osteocalcin (bglap, osteopontin (SPP1, secreted protein acidic and rich in cysteine (SPARC and type I collagen α1 chain (COL1A1 have been studied in gilthead sea bream. Results During gilthead sea bream larval development, specific expression profiles for each gene were tightly regulated during fish morphogenesis and correlated with specific morphogenetic events and tissue development. Dietary hypervitaminosis A during early larval development disrupted the normal gene expression profile for genes involved in RA signalling (RARA, VA homeostasis (RBP and several genes encoding ECM proteins that are linked to skeletogenesis, such as bglap and mgp. Conclusions Present data reflects the specific gene expression patterns of several genes involved in larval fish RA signalling and skeletogenesis; and how

  1. Age and Growth Characteristics of Crimson Sea Bream Paragyrops edita Tanaka in Beibu Gulf

    Institute of Scientific and Technical Information of China (English)

    HOU Gang; FENG Bo; LU Huosheng; ZHU Junfeng

    2008-01-01

    Age and growth characteristics of crimson sea bream Paragyrops edita Tanaka in Beibu Gulf were studied through bot-tom trawling and gillnet fleets fishing from July 2006 to December 2007.A total number of 1155 individuals,ranging from 49 to 249mmin standard length was examined.The age of the fish was determined from sagittal otoliths.One year growth was made up of onetranslucent and one opaque zone.A maximum likelihood estimation procedure was used to fit the Von Bertalanffy,Logistic andGompertz growth functions to the length-at-age data.ARSS indicated that there were no significant differences in growth betweensexes in the three growth models (P>0.05),and the Von Bertalanffy growth function L=292.8{1-exp[-0.167(t+1.116)]} was se-lected as the most appropriate growth model according to Akaike's information criterion (AIC).

  2. Detection of Range Finding Test of Mercury Chloride in Yellowfin Sea Bream (Acanthopagrus latus

    Directory of Open Access Journals (Sweden)

    Aliakbar Hedayati

    2010-07-01

    Full Text Available Toxicity tests allow the determination of pollution effects, providing direct evidence of the biological responses of marine organisms to contaminants. Fifty four yellow fine sea bream all immature male in same size (120 g final body weight average were obtained from Mahshahr creeks with hooks in a Upon capture. In laboratory Fish maintained in a seawater re-circulatory system (300-L tanks equipped with physical/biological filters and with aeration. All samples were acclimated for one weeks in a 15 aerated fiberglass tank containing 46 ppt saltwater maintained at 25 C under a constant 12:12 L:D photoperiod. Acclimatized Fish were fed daily with a live feed (fresh shrimp and daily we check water quality and water parameters. Hg2+ tested concentrations were 20, 50, 100, 200, 500, 1000, 2000, 5000 and10000 µg/l, Groups of six male yellow fine sea bream (120 g were exposed for 96 h to each of the Range Finding Test for LC50, in fiberglass tank equipped with aeration with 100 l of test medium. The control group was exposed to filtered sea water in similar conditions. Test medium was not renewed during the assay and no food was provided to the animals. Values of pH, Temperature, and salinity were measured at time 0, 24, 48, 72 and 96 h. At the end of the bioassay, Range values were determined as 500-1000 µg/l (fifty percent of mortality between 500 and 1000. Range Finding Test values indicated that mercury is more toxic to A. latus. Range obtained in the present study compare with corresponding values that have been published in the literature for other species of fish, show different Range of mercury in different species and even different time, but what is important, lower value of Range Finding Test for A. latus compare with most species and confirm sensitively of A. latus to low mercury doses.

  3. First record of red filament threadfin bream, Nemipterus marginatus (Valenciennes, 1830) (Perciformes, Nemipteridae),from Chinese waters

    Institute of Scientific and Technical Information of China (English)

    NING Ping; WU Renxie; LIU Jing

    2011-01-01

    We collected five specimens of threadfin bream from Beihai,Guangxi,China in March 2010.These were subsequently identified as red filament threadfin bream Nemipterus marginatus (Valenciennes,1830),being the first record of this species from Chinese waters.N.marginatus is distinguished by the following characteristics:lower border of eye lies above a line from tip of snout to upper base of pectoral fin; mouth oblique,maxillary extending to lower anterior border of pupil; teeth in jaws in several rows,pointed; upper jaw with 3 to 5 pairs of small recurved canines; suborbital with straight lower edge and rounded posterior edge; pectoral fins extending to between level of anus and origin of anal fin; pelvic fins reaching to the first or second anal rays; caudal fm forked,upper lobe tails into a short reddish filament; dorsal fin bluish with a yellow margin distally and a broad yellow median band which subdivides posteriorly into 3 small bands.

  4. Diel activity and variability in habitat use of white sea bream in a temperate marine protected area.

    Science.gov (United States)

    Di Lorenzo, Manfredi; Fernández, Tomás Vega; Badalamenti, Fabio; Guidetti, Paolo; Starr, Richard M; Giacalone, Vincenzo Maximiliano; Di Franco, Antonio; D'Anna, Giovanni

    2016-05-01

    Fish populations are often comprised of individuals that use habitats and associated resources in different ways. We placed sonic transmitters in, and tracked movements of, white sea bream (Diplodus sargus sargus) in the no-take zone of a Mediterranean marine protected area: the Torre Guaceto marine protected area, (Adriatic Sea, Italy). Tagged fish displayed three types of diel activity patterns in three different habitats: sand, rocky reefs and "matte" of the seagrass Posidonia oceanica. Individuals were more active during the day than at night. Overall, white sea bream displayed a remarkable behavioural plasticity in habitat use. Our results indicate that the observed behavioural plasticity in the marine protected area could be the result of multiple ecological and environmental drivers such as size, sex and increased intra-specific competition. Our findings support the view that habitat diversity helps support high densities of fishes. PMID:26922044

  5. The behaviour and ecology of adult common bream Abramis brama (L.) in a heavily modified lowland river

    OpenAIRE

    Gardner, Christopher John

    2013-01-01

    Anthropogenic influences on riverine ecosystems have led to numerous impacts that have compromised fish communities. Large European lowland rivers have been amongst the most impacted aquatic environments globally. However, paradoxically, these environments have also been amongst the least studied, due the inherent difficulties of studying fish in large, turbid, dynamic environments. Common bream Abramis brama (L.) populations are amongst those impacted by reduced lateral and longitudinal conn...

  6. Mitochondrial Genome Variation after Hybridization and Differences in the First and Second Generation Hybrids of Bream Fishes

    Science.gov (United States)

    Zhang, Wei-Zhuo; Xiong, Xue-Mei; Zhang, Xiu-Jie; Wan, Shi-Ming; Guan, Ning-Nan; Nie, Chun-Hong; Zhao, Bo-Wen; Hsiao, Chung-Der; Wang, Wei-Min; Gao, Ze-Xia

    2016-01-01

    Hybridization plays an important role in fish breeding. Bream fishes contribute a lot to aquaculture in China due to their economically valuable characteristics and the present study included five bream species, Megalobrama amblycephala, Megalobrama skolkovii, Megalobrama pellegrini, Megalobrama terminalis and Parabramis pekinensis. As maternal inheritance of mitochondrial genome (mitogenome) involves species specific regulation, we aimed to investigate in which way the inheritance of mitogenome is affected by hybridization in these fish species. With complete mitogenomes of 7 hybrid groups of bream species being firstly reported in the present study, a comparative analysis of 17 mitogenomes was conducted, including representatives of these 5 bream species, 6 first generation hybrids and 6 second generation hybrids. The results showed that these 17 mitogenomes shared the same gene arrangement, and had similar gene size and base composition. According to the phylogenetic analyses, all mitogenomes of the hybrids were consistent with a maternal inheritance. However, a certain number of variable sites were detected in all F1 hybrid groups compared to their female parents, especially in the group of M. terminalis (♀) × M. amblycephala (♂) (MT×MA), with a total of 86 variable sites between MT×MA and its female parent. Among the mitogenomes genes, the protein-coding gene nd5 displayed the highest variability. The number of variation sites was found to be related to phylogenetic relationship of the parents: the closer they are, the lower amount of variation sites their hybrids have. The second generation hybrids showed less mitogenome variation than that of first generation hybrids. The non-synonymous and synonymous substitution rates (dN/dS) were calculated between all the hybrids with their own female parents and the results indicated that most PCGs were under negative selection. PMID:27391325

  7. IMPACT OF OOCYTE SIZE ON LHRHa INDUCED OVULATION AND FERTILIZED EGG QUALITY IN SADDLED BREAM OBLADA MELANURA (LINNAEUS, 1758)

    OpenAIRE

    Nenad Antolović; Valter Kožul; Nikša Glavić; Jakša Bolotin

    2013-01-01

    The objective of this study was to evaluate the effects of oocyte size and luteinizing hormone releasing hormone analogue (LHRHa) on ovulatory success in artificial fertilization. Vitellogenic females with maximum oocyte diameters 400-550 µm were repeatedly injected with LHRHa (20 µg kg-1 per injection). Fish with maximum oocyte diameters 500 µm spawned within 48-54 h. These results demonstrate that injected LHRHa is effective for ovulation of saddled bream with maximum oocyte diameters >500µ...

  8. Comparison of genetic variation of wild and farmed Bream (Abramis brama orientalis; berg, 1905 using microsatellite markers

    Directory of Open Access Journals (Sweden)

    Zeinab Hosseinnia

    2014-09-01

    Full Text Available Bream (Abramis brama orientalis is one of the most commercially valuable fish in the Caspian Sea. The aim of this study was to compare levels of genetic polymorphism between wild and farmed Bream populations using seven microsatellite loci. Genetic diversity was investigated by studying samples collected from two regions; Chaboksar and the Artificial Propagation Center of Guilan province. Allele frequency was found to have declined in wild and cultured fish due to inbreeding and genetic drift. Significant population differentiation (Fst was observed between wild and farmed populations, which could be explained by the low number of alleles in two populations. Significant deviations from the Hardy-Weinberg equilibrium were found at more loci. Beyond the null alleles' hypothesis, heterozygote deficiency may have arisen due to inbreeding. Both populations showed lowest genetic diversity according to the number of alleles and genotypes per each locus. This approach was carried out for the first time and could provide information regarding the genetic variability of farmed and wild abramis brama fish using microsatellite markers. Results could be used for the management and conservation of artificial Bream propagation programs.

  9. The testis and ovary transcriptomes of the rock bream (Oplegnathus fasciatus: A bony fish with a unique neo Y chromosome

    Directory of Open Access Journals (Sweden)

    Dongdong Xu

    2016-03-01

    Full Text Available The rock bream (Oplegnathus fasciatus is considerably one of the most economically important marine fish in East Asia and has a unique neo-Y chromosome system that is a good model to study the sex determination and differentiation in fish. In the present study, we used Illumina sequencing technology (HiSeq2000 to sequence, assemble and annotate the transcriptome of the testis and ovary tissues of rock bream. A total of 40,004,378 (NCBI SRA database SRX1406649 and 53,108,992 (NCBI SRA database SRX1406648 high quality reads were obtained from testis and ovary RNA sequencing, respectively, and 60,421 contigs (with average length of 1301 bp were obtained after de novo assembling with Trinity software. Digital gene expression analysis reveals 14,036 contigs that show gender-enriched expressional profile with either testis-enriched (237 contigs or ovary-enriched (581 contigs with RPKM >100. There are 237 male- and 582 female-abundant expressed genes that show sex dimorphic expression. We hope that the gonad transcriptome and those gender-enriched transcripts of rock bream can provide some insight into the understanding of genome-wide transcriptome profile of teleost gonad tissue and give useful information in fish gonad development.

  10. Chemical contamination of the Rybinsk Reservoir, northwest Russia: Relationship between liver polychlorinated biphenyls (PCB) content and health indicators in bream (Abramis brama)

    Science.gov (United States)

    Chuiko, G.M.; Tillitt, D.E.; Zajicek, J.L.; Flerov, B.A.; Stepanova, V.M.; Zhelnin, Y.Y.; Podgornaya, V.A.

    2007-01-01

    The Rybinsk Reservoir (Russia) is the largest artificial waterbody in Europe (4550 km2) and provides drinking water for population of the cities located along the coast line. Industrialization in Cherepovets at the northeastern portion of the reservoir, including one of the largest metallurgical facilities in Europe, has resulted in chemical contamination of the reservoir. The extent of polychlorinated biphenyls (PCB) contamination in bream liver, a common fish species, taken from six locations in the Rybinsk Reservoir and Volga River, and biochemical and morphometric biomarkers of fish health were investigated. Liver PCB concentrations ranged from non-detected to 3.4 ??g/g wet wt of liver, with the greatest concentrations found in fish taken near the industrialized area in Sheksna Reach of Rybinsk Reservoir. The source of the bream contamination is the PCB pollution of bottom organisms and sediments conditioned with industrialization facilities of Cherepovets. The patterns of the PCB congeners in the livers of bream taken near Cherepovets were similar at all of the stations that were sampled around the reservoir and Volga River. Among the common fish health biomarkers used only liver total ChE activity and liver-somatic index in bream near Cherepovets can reflect environmental pollution. Other morphometric (FCF, Clark's condition factors, and spleen-somatic index) and biochemical (protein content and acetylcholinesterase activity in the brain) biomarkers related with fish health varied among locations, but were not correlated to the concentrations of PCBs in the bream livers. ?? 2006 Elsevier Ltd. All rights reserved.

  11. Taurine and fish development: insights for the aquaculture industry.

    Science.gov (United States)

    Pinto, Wilson; Rønnestad, Ivar; Dinis, Maria Teresa; Aragão, Cláudia

    2013-01-01

    Expansion of the aquaculture industry is limited by incomplete knowledge on fish larval nutritional requirements. Nevertheless, it is believed that dietary taurine deficiencies may be particularly critical for fish larvae. The reasons include the high taurine levels found during egg and yolk-sac stages of fish, suggesting that taurine may be of pivotal importance for larval development. Moreover, unlike aquaculture feeds, natural preys of fish larvae contain high taurine levels, and dietary taurine supplementation has been shown to increase larval growth in several fish species. This study aimed to further explore the physiological role of taurine during fish development. Firstly, the effect of dietary taurine supplementation was assessed on growth of gilthead sea bream (Sparus aurata) larvae and growth, metamorphosis success and amino acid metabolism of Senegalese sole (Solea senegalensis) larvae. Secondly, the expression of taurine transporter (TauT) was characterised by qPCR in sole larvae and juveniles. Results showed that dietary taurine supplementation did not increase sea bream growth. However, dietary taurine supplementation significantly increased sole larval growth, metamorphosis success and amino acid retention. Metamorphosis was also shown to be an important developmental trigger to promote taurine transport in sole tissues, while evidence for an enterohepatic recycling pathway for taurine was found in sole at least from juvenile stage. Taken together, our studies showed that the dependence of dietary taurine supplementation differs among fish species and that taurine has a vital role during the ontogenetic development of flatfish, an extremely valuable group targeted for aquaculture production. PMID:23392894

  12. Relationships between circulating androgens, aggressive behaviour and breeding tubercles in males of the common bream Abramis brama L. in an aquarium environment.

    Science.gov (United States)

    Poncin, P; Matondo, B Nzau; Termol, C; Kestemont, P; Philippart, J C

    2011-09-01

    In this study, relationships between circulating androgens, aggressive behaviour and breeding tubercles in males of common bream Abramis brama were examined in an aquarium environment. The breeding tubercles of fish were counted, the number of attacks was quantified by male status and circulating rates of testosterone and 11-ketotestosterone from blood plasma were analysed using radioimmunoassay procedures. The results revealed that no significant differences were found between circulating testosterone and 11-ketotestosterone in territorial and nonterritorial males. Furthermore, no significant correlations were found between circulating androgens, androgens and aggression, androgens and tubercles and breeding tubercles and aggression in common bream by male status. However, territorial fish displayed a significantly higher level of aggressive behaviour and breeding tubercles than nonterritorial fish. In natural environments, the occurrence of breeding tubercles during the spawning season could contribute to identifying the behavioural status of common bream males. PMID:21132526

  13. Moon phase influences the diet of southern Ray's bream Brama australis.

    Science.gov (United States)

    Horn, P L; Forman, J S; Dunn, M R

    2013-04-01

    Diet composition of the southern Ray's bream Brama australis was examined from stomach contents of 399 specimens sampled by bottom trawl on Chatham Rise to the east of South Island, New Zealand, over 3 years. Prey items were predominantly mesopelagic fishes and crustaceans. Multivariate analysis indicated that moon phase explained more of the diet variability than any other predictor examined. It appears likely that diet composition is influenced by a combination of changes in both tidal flows and illumination. Different combinations of prey were consumed by B. australis at different times of the lunar cycle. An influence of moon phase on feeding by fishes has rarely been reported, but it is likely that moon phase influences the diets of other species that specialize in mesopelagic prey. The most important prey group by mass for B. australis was Myctophidae (primarily Lampanyctodes hectoris), followed by Stomiiformes (primarily Maurolicus australis) and shrimps (Sergestes spp). An ontogenetic shift in diet was observed, from numerical dominance by small crustaceans including amphipods and euphausiids (with some fishes) in smaller (mass 1440 g) B. australis. PMID:23557312

  14. Effect of dietary docosahexaenoic acid on lipogenesis and lipolysis in black sea bream, Acanthopagrus schlegeli

    Institute of Scientific and Technical Information of China (English)

    JI Hong; OM Ahamd Daud; YOSHIMATSU Takao; UMINO Testuya; NAKAGAWA Heisuke; FURUHASHI Makoto; SAKAMOTO Shuichi

    2007-01-01

    Hatchery-reared juvenile black sea breams are characterized by a low level of highly unsaturated fatty acids in their bodies, as compared with wild fish. To assess the effect of docosahaxaenoic acid (DHA) on lipogenic and lipolysis enzymes, one-year fish were reared on a casein-based purified diet and a DHA fortified diet (1.5% DHA ethyl ester/kg diet) for 60 d, followed with a period of 55 d for starvation. Dietary DHA was effectively incorporated into the fish body. Fortification of DHA depressed activities of glucose-6-phosphate dehydrogenase and NADP-isocitrate dehydrogenase as lipogenic enzymes in the hepatopancreas and intraperitoneal fat body. Carnitine palmitoyltransferase as lipolysis enzyme in the hepatopancreas was active in the DHA fortified fish. Starvation after feeding experiment induced increased carnitine palmitoyltransferase activity in both control and DHA fortified fish and the activity remained higher in the DHA fortified fish, while the monoenes were selectively consumed prior to highly unsaturated fatty acids. These results indicated that dietary DHA depressed lipogenesis and activated lipolysis.

  15. Microsatellite Development for an Endangered Bream Megalobrama pellegrini (Teleostei, Cyprinidae Using 454 Sequencing

    Directory of Open Access Journals (Sweden)

    Zuogang Peng

    2012-03-01

    Full Text Available Megalobrama pellegrini is an endemic fish species found in the upper Yangtze River basin in China. This species has become endangered due to the construction of the Three Gorges Dam and overfishing. However, the available genetic data for this species is limited. Here, we developed 26 polymorphic microsatellite markers from the M. pellegrini genome using next-generation sequencing techniques. A total of 257,497 raw reads were obtained from a quarter-plate run on 454 GS-FLX titanium platforms and 49,811 unique sequences were generated with an average length of 404 bp; 24,522 (49.2% sequences contained microsatellite repeats. Of the 53 loci screened, 33 were amplified successfully and 26 were polymorphic. The genetic diversity in M. pellegrini was moderate, with an average of 3.08 alleles per locus, and the mean observed and expected heterozygosity were 0.47 and 0.51, respectively. In addition, we tested cross-species amplification for all 33 loci in four additional breams: M. amblycephala, M. skolkovii, M. terminalis, and Sinibrama wui. The cross-species amplification showed a significant high level of transferability (79%–97%, which might be due to their dramatically close genetic relationships. The polymorphic microsatellites developed in the current study will not only contribute to further conservation genetic studies and parentage analyses of this endangered species, but also facilitate future work on the other closely related species.

  16. The impact of an oil spill on organs of bream Abramis brama in the Po River.

    Science.gov (United States)

    Giari, L; Dezfuli, B S; Lanzoni, M; Castaldelli, G

    2012-03-01

    An oil spill into the River Lambro occurred on 23 February 2010 and reached the Po River the following day. Breams captured here on 1 March 2010, along with a sample from a control site, were examined by light and electron microscopy. The main affected organs were skin and gill with slight or no damage to liver, kidney, and intestine. The gills exhibited lamellar aneurisms, fusion of secondary lamellae, edema with epithelial lifting, mucous cell hypertrophy, and mucus hypersecretion. Significantly higher mucous cell density was observed in the skin of exposed fish. Histochemical staining revealed that acid glycoconjugates were prevalent in epidermal mucous cells in the exposed Abramis brama, whereas neutral and mixed glycoconjugates were dominant in the control fish. Rodlet cells were significantly more abundant in the kidney of exposed fish and showed ultrastructural differences compared to controls. These histopathologic effects were indicators of chemical stress due to exposure to oil. The present study is one of the first which explores the acute effects of this incident and makes part of a few reports focused on freshwater oil spill. PMID:22030380

  17. Kudoa unicapsula n. sp. (Myxosporea: Kudoidae) a parasite of the Mediterranean mullets Liza ramada and L. aurata (Teleostei: Mugilidae).

    Science.gov (United States)

    Yurakhno, V M; Ovcharenko, M O; Holzer, A S; Sarabeev, V L; Balbuena, J A

    2007-11-01

    A new multivalvulid myxozoan parasite, Kudoa unicapsula n. sp., is described from the intestinal mesentery, intestine and pyloric caeca of the thin-lipped grey mullet Liza ramada (Risso 1826) and the golden grey mullet L. aurata (Risso, 1810) from the Mediterranean coastal waters of Spain. It is characterized by the presence of elongated, rice corn-like white cysts of 0.47-0.56 x 0.18-0.38 mm, filled with tetracapsulate, slightly asymmetric spores, rectangular in apical view and tear-shaped in lateral view with four polar capsules of considerably different size and slightly unequal spore valves with rounded edges, overlapping each other on the apex of the spore. One large polar capsule includes a polar filament coiled in two to three turns, and the other three polar capsules, which are very small, posses only a rudimental filament. Both light and electron microscopy data showed that this species differs from all previously described Kudoa spp. with unequal polar capsules. The molecular analysis based on 18S and 28S ribosomal deoxyribonucleic acid DNA sequence data of K. unicapsula n. sp. indicates a close relationship and thus phylogenetic clustering together with K. trifolia, a myxozoan from the same host and the same geographical location. PMID:17846792

  18. Effects of α-tocopheryl acetate supplementation in preslaughter diet on antioxidant enzyme activities and fillet quality of commercial-size Sparus macrocephalus *

    OpenAIRE

    Zhang, Xiao-Dong; Wu, Tian-xing; Cai, Li-sheng; Zhu, Yong-fei

    2007-01-01

    This study examined the effects of dietary α-tocopheryl acetate supplementation on antioxidant enzyme activities and fillet quality in commercial-size Sparus macrocephalus. Three hundred fish [main initial weight (350±12) g] were divided into three groups (E250, E500 and E1000) and reared in 9 cages. The fish were fed for 8 weeks with three diets containing different levels of dietary α-tocopheryl acetate (289, 553, 1 069 mg/kg). Over the experimental period, fish were fed to satiation and re...

  19. Changes in the contents of strontium, barium, and lead in scales of bream Abramis brama from the Mozhaisk Reservoir over a quarter century.

    Science.gov (United States)

    Saltykova, E A; Pelgunova, L A; Sokolova, E L; Skomorokhov, M O; Demidova, T B; Golubtsov, A S

    2016-03-01

    The heavy metal contents in the scales of bream (Abramis brama) from the Mozhaisk Reservoir collected in the second half of the 1980s were compared to the current values. The concentrations of three out of the seven elements studied in the bream scales have changed severalfold during the past quarter century: that of strontium has decreased, and those of barium and lead have increased. Short-term variations of heavy metal contents have proved to be smaller than the observed long-term differences. There is grounds to believe that these long-term differences adequately reflect the changes that have occurred in the water body. PMID:27193879

  20. Dynamics of the content of H2O, Na, K, Ca and Mg in the eggs of bream, Abramis brama L. in natural conditions and under stress

    OpenAIRE

    Vladimir Ivanovich Martemyanov

    2016-01-01

    Objective: To study the dynamics of H2O, Na, K, Ca and Mg content in the oocytes of bream, Abramis brama observed in prespawning period on breeding ground and in reply to catching, transportation and subsequent remaining of spawners in a cage. Methods: For research on the dynamics of H2O and cations content in the oocytes of bream females, Abramis brama in the course of eggs transition from maturity Stage IV to Stage V, fishes were caught from breeding ground in the coastal zon...

  1. A New Chiroptera Record in Guangxi,China:Murina aurata%广西翼手目一新纪录——金管鼻蝠

    Institute of Scientific and Technical Information of China (English)

    李友邦; Neil M.Furey; 韦龙韬

    2010-01-01

    2009年11月,在广西西北部木论国家级自然保护区小洞丹洞穴中采到一翼手目标本,经鉴定为金管鼻蝠Murina aurata,是广西翼手目新纪录.目前,在木论自然保护区的18个洞穴调查中,只在该洞发现有分布.标本现保存于广西师范大学生物多样性标本馆.

  2. Molecular cloning and expression analysis of interleukin (IL)-15 and IL-15 receptor α from rock bream, Oplegnathus fasciatus.

    Science.gov (United States)

    Bae, Jin-Sol; Shim, Sang Hee; Hwang, Seong Don; Kim, Ju-Won; Park, Dae-Won; Park, Chan-Il

    2013-10-01

    Mammalian interleukin (IL)-15 plays an important role in the activation of CD8(+) T cells and natural killer (NK) cells along with its receptor α (IL-15Rα). To understand the potential roles of IL-15 and IL-15Rα in fish, we identified IL-15 and IL-15Rα cDNA from rock bream (Oplegnathus fasciatus) and investigated their gene expression profiles after bacterial and viral infection. Coding regions of rock bream (Rb) IL-15 and RbIL-15Rα cDNAs were 534 and 402 bp encoding 177 and 133 amino acid residues, respectively. The sushi domain of IL-15Rα was highly conserved between rock bream and other species. Unlike other IL-15Rαs, RbIL-15Rα does not have a transmembrane region. Gene expression of RbIL-15 and RbIL-15Rα was widely expressed in different tissues of healthy fish, especially immune-related tissues. RbIL-15 and RbIL-15Rα were highly induced in the kidney and spleen after infection with Edwardsiella tarda, Streptococcus iniae and red seabream iridovirus. Gene expression patterns of RbIL-15 and RbIL-15Rα were similar in the kidney and spleen after pathogen infection. However, these genes were differentially induced in the liver after pathogen infection. These results suggest that the different responses of RbIL-15 and RbIL-15Rα to pathogen infection may be induced by different tissues or cell types. PMID:23911652

  3. Feeding effect of selenium enriched rotifers on larval growth and development in red sea bream Pagrus major

    OpenAIRE

    Kim, Hee-Jin; Sakakura, Yoshitaka; Maruyama, Isao; Nakamura, Toshio; Takiyama, Kazushi; Fujiki, Haruyuki; Hagiwara, Atsushi

    2014-01-01

    Feeding trials were conducted to investigate the effect of selenium (Se)-enriched rotifers on growth and development of red sea bream Pagrus major larvae. Fish were reared from fertilized eggs (98% hatch rate) to 20. days post hatch (dph) at 19. °C with two different food sources; non-enriched S-type rotifers (0.0. μg. Se/g D.W., control diet) or Se-enriched rotifers (2.2. μg. Se/g D.W., Se-enriched diet) at 10. rotifers/mL, respectively. On the last day of larviculture, the Se-enriched diet ...

  4. Design and Application of a Solar Mobile Pond Aquaculture Water Quality-Regulation Machine Based in Bream Pond Aquaculture.

    Directory of Open Access Journals (Sweden)

    Xingguo Liu

    Full Text Available Bream pond aquaculture plays a very important role in China's aquaculture industry and is the main source of aquatic products. To regulate and control pond water quality and sediment, a movable solar pond aquaculture water quality regulation machine (SMWM was designed and used. This machine is solar-powered and moves on water, and its primary components are a solar power supply device, a sediment lifting device, a mechanism for walking on the water's surface and a control system. The solar power supply device provides power for the machine, and the water walking mechanism drives the machine's motion on the water. The sediment lifting device orbits the main section of the machine and affects a large area of the pond. Tests of the machine's mechanical properties revealed that the minimum illumination necessary for the SMWM to function is 13,000 Lx and that its stable speed on the water is 0.02-0.03 m/s. For an illumination of 13,000-52,500 Lx, the sediment lifting device runs at 0.13-0.35 m/s, and its water delivery capacity is 110-208 m(3/h. The sediment lifting device is able to fold away, and the angle of the suction chamber can be adjusted, making the machine work well in ponds at different water depths from 0.5 m to 2 m. The optimal distance from the sediment lifting device to the bottom of the pond is 10-15 cm. In addition, adjusting the length of the connecting rod and the direction of the traction rope allows the SMWM to work in a pond water area greater than 80%. The analysis of water quality in Wuchang bream (Parabramis pekinensis and silver carp (Hypophthalmichthys molitrix culture ponds using the SMWM resulted in decreased NH3(+-N and available phosphorus concentrations and increased TP concentrations. The TN content and the amount of available phosphorus in the sediment were reduced. In addition, the fish production showed that the SMWM enhanced the yields of Wuchang bream and silver carp by more than 30% and 24%, respectively. These

  5. Design and Application of a Solar Mobile Pond Aquaculture Water Quality-Regulation Machine Based in Bream Pond Aquaculture.

    Science.gov (United States)

    Liu, Xingguo; Xu, Hao; Ma, Zhuojun; Zhang, Yongjun; Tian, Changfeng; Cheng, Guofeng; Zou, Haisheng; Lu, Shimin; Liu, Shijing; Tang, Rong

    2016-01-01

    Bream pond aquaculture plays a very important role in China's aquaculture industry and is the main source of aquatic products. To regulate and control pond water quality and sediment, a movable solar pond aquaculture water quality regulation machine (SMWM) was designed and used. This machine is solar-powered and moves on water, and its primary components are a solar power supply device, a sediment lifting device, a mechanism for walking on the water's surface and a control system. The solar power supply device provides power for the machine, and the water walking mechanism drives the machine's motion on the water. The sediment lifting device orbits the main section of the machine and affects a large area of the pond. Tests of the machine's mechanical properties revealed that the minimum illumination necessary for the SMWM to function is 13,000 Lx and that its stable speed on the water is 0.02-0.03 m/s. For an illumination of 13,000-52,500 Lx, the sediment lifting device runs at 0.13-0.35 m/s, and its water delivery capacity is 110-208 m(3)/h. The sediment lifting device is able to fold away, and the angle of the suction chamber can be adjusted, making the machine work well in ponds at different water depths from 0.5 m to 2 m. The optimal distance from the sediment lifting device to the bottom of the pond is 10-15 cm. In addition, adjusting the length of the connecting rod and the direction of the traction rope allows the SMWM to work in a pond water area greater than 80%. The analysis of water quality in Wuchang bream (Parabramis pekinensis) and silver carp (Hypophthalmichthys molitrix) culture ponds using the SMWM resulted in decreased NH3(+)-N and available phosphorus concentrations and increased TP concentrations. The TN content and the amount of available phosphorus in the sediment were reduced. In addition, the fish production showed that the SMWM enhanced the yields of Wuchang bream and silver carp by more than 30% and 24%, respectively. These results

  6. Effects of α-tocopheryl acetate supplementation in preslaughter diet on antioxidant enzyme activities and fillet quality of commercial-size Sparus macrocephalus

    Institute of Scientific and Technical Information of China (English)

    2007-01-01

    This study examined the effects of dietary α-tocopheryl acetate supplementation on antioxidant enzyme activities and fillet quality in commercial-size Sparus macrocephalus. Three hundred fish [main initial weight (350±12) g] were divided into three groups (E250, E500 and E1000) and reared in 9 cages. The fish were fed for 8 weeks with three diets containing different levels of dietary α-tocopheryl acetate (289, 553, 1069 mg/kg). Over the experimental period, fish were fed to satiation and reached a final mean weight of (465±28) g without significant body weight difference and proximate composition difference. Fillet α-tocopherol was significantly (P<0.05) different between groups, reaching levels of 14.2, 22.1, 30.9 μg/mg fillet for groups E250,E500 and E1000, respectively. Total serum superoxide dismutase (SOD) activity increased significantly (P<0.05) in fish fed the diets high in α-tocopheryl acetate, but serum glutathione peroxidase (GPX) activity was unaffected. In storage on ice, fillets offish fed the diets high in α-tocopheryl acetate exhibited significantly lower (P<0.05) levels of oxidation. These results suggested that increased dietary α-tocopheryl acetate could increase its flesh deposition, increase the activity of SOD and prevent lipid peroxidation of Sparus macrocephalus fillets in retail storage on ice.

  7. Induction of gynogenesis in red crucian carp using spermatozoa of blunt snout bream

    Institute of Scientific and Technical Information of China (English)

    SUN Yuandong; LIU Yun; TAO Min; LIU Shaojun; ZHANG Chun; DUAN Wei; SHEN Jiamin; WANG Jing; ZENG Chen; LONG Yu

    2007-01-01

    Gynogenetic diploid was induced in red crucian carp (RCC) ( Carassius auratus Red Variety) eggs using UV-irradiated spermatozoa from blunt snout bream (B) (Megalobrama amblycephala ) or from mirror carp (C) (Cyprinus carpio. L). Spermatozoa were genetically inactivated by an appropriate UV dosage, and then the maternal DNA was duplicated with cold shock at 0-4 ℃. When using the spermatozoa of B, the fertilization rate, hatching rate and survival at first feeding were 52.6 ± 3.0 %, 23.6 ± 4.1 % and 15.7 ± 3.4 %, respectively, and the survival at first feeding was significantly higher than that ( 11.3 ± 2.2 % ) when using the spermatozoa of C (Cyprinus carpio. L). According to the morphological characteristics, the chromosome number and the degree of gonadal development, gynogenetic RCC could be distinguished from the control hybrids of RCC♀ × B ♂ . The individuals with red body color, 100 chromosomes and normal gonadal development were successful gynogenetic RCC, while the individuals with 124 or 148 chromosomes and delayed gonadal development were hybrids of (RCC × B). The triploid hybrids (RCC × B) (2 years old) were sterile, but the tetraploid hybrids (RCC × B) were sexually mature age of two. In the present study, compared to the spermatozoa of C, the advantages of spermatozoa of B as the activation source were that could increase the survival at first feeding of gynogenetic individuals and simplify the confirmation of gynogenetic status, which suggested that the spermof B was an effective activation source for inducing gynogenesis in crucian carp.

  8. Food resources utilization by bream (IAbramis brama/I) and roach (IRutilus rutilus/I) in a canyon-shaped reservoir

    Czech Academy of Sciences Publication Activity Database

    Vašek, Mojmír

    Liběchov: Ústav živočišné fyziologie a genetiky, 2001. s. 186. [European Congress of Ichthyology: In the heart of Europe! /10./. 03.09.2001-07.09.2001, Praha] Institutional research plan: CEZ:AV0Z6017912 Keywords : bream * roach * diet composition Subject RIV: EH - Ecology, Behaviour

  9. [The nature of changes of some immunophysiological characteristics in bream (Abramis brama) infected with plerocercoids (Ligula intestinalis) at various stages of parasite development].

    Science.gov (United States)

    Silkina, N I; Mikriakov, V R; Mikriakov, D V

    2012-01-01

    The data from studies of the antimicrobial properties of blood serum, the content of total lipids, and antioxidant activity of immunocompetent tissues and organs of breams Abramis brama infected with plerocercoids Ligula intestinalis depending on the phase of development of the parasite are presented. The quantitative characteristics of the studied parameters are determined. PMID:23136746

  10. PCDDs, PCDFs, and dioxin-like PCBs in breams (Abraims brama) from German rivers. Results from the German Environmental Specimen Bank

    Energy Technology Data Exchange (ETDEWEB)

    Schroeter-Kermani, C. [Federal Environmental Agency, Berlin (Germany); Herrmann, T.; Paepke, O. [ERGO Forschungsgesellschaft mbH, Hamburg (Germany); Stachel, B. [Wasserguetestelle Elbe der Arbeitsgemeinschaft fuer die Reinhaltung der Elbe, Hamburg (Germany)

    2004-09-15

    In July 2002 a new regulation of the European Commission came into force setting maximum permitted values for PCDD/Fs in food. Until the end of 2004 these limit values have to be verified with current monitoring data, especially in regard to the integration of dioxin-like PCBs in this regulation. It is well known that fish accumulate lypophilic substances to a high degree from their aquatic surroundings so that fish and fish products are an important source of human intake of dioxins. Several studies have been published recently regarding the contamination of marine fish species with PCDD/Fs and dioxin-like PCBs, but less is known about the contamination of fresh water fish with these substances. To bridge this gap, the German environmental specimen bank (ESB) analyzed archived bream muscle samples for PCDD/Fs and dioxin-like PCBs. Breams are widely distributed in Europe and often used as a monitoring organism for fresh water and sediment contamination because of their small migration radius. As adult breams feed mainly on benthic invertebrates they are permanently in direct contact with the sediments. Although bream is less consumed at least in Germany the here presented results might facilitate general statements regarding the contamination of other fresh water fish species with PCDD/Fs and dioxin-like PCBs.

  11. The sub-cellular fate of mercury in the liver of wild mullets (Liza aurata) – Contribution to the understanding of metal-induced cellular toxicity

    International Nuclear Information System (INIS)

    Highlights: • This study is one of the first reports of Hg sub-cellular partitioning in wild fish. • Hg partition in Liza aurata liver differed between reference and contaminated sites. • Levels of Hg in sub-cellular fractions were in line with environmental contamination. • Lysosomes plus microsomes was the main fraction bounding Hg at the contaminated site. • Sub-cellular partitioning of Hg revealed to be a promising indicator of cellular toxicity. - Abstract: Mercury is a recognized harmful pollutant in aquatic systems but still little is known about its sub-cellular partitioning in wild fish. Mercury concentrations in liver homogenate (whole organ load) and in six sub-cellular compartments were determined in wild Liza aurata from two areas – contaminated (LAR) and reference. Water and sediment contamination was also assessed. Fish from LAR displayed higher total mercury (tHg) organ load as well as in sub-cellular compartments than those from the reference area, reflecting environmental differences. However, spatial differences in percentage of tHg were only observed for mitochondria (Mit) and lysosomes plus microsomes (Lys + Mic). At LAR, Lys + Mic exhibited higher levels of tHg than the other fractions. Interestingly, tHg in Mit, granules (Gran) and heat-denaturable proteins was linearly correlated with the whole organ. Low tHg concentrations in heat stable proteins and Gran suggests that accumulated levels might be below the physiological threshold to activate those detoxification fractions

  12. Heavy metal accumulation in Diplodus annularis, Liza aurata, and Solea vulgaris relevant to their concentration in water and sediment from the southwestern Mediterranean (coast of Sfax).

    Science.gov (United States)

    Ben Salem, Zohra; Ayadi, Habib

    2016-07-01

    The concentrations of heavy metals (Cd, Cu, Fe, Pb, Ni, and Zn) were measured in the liver, gills, and muscle of Solea vulgaris, Liza aurata, and Diplodus annularis, collected from the south coast of Sfax (Gabes Gulf, southwestern Mediterranean). The concentrations of heavy metals in water exhibited the following decreasing order (expressed in μg l(-1)): Fe > Ni > Zn > Cu > Pb > Cd whereas the trend is somewhat different in sediments (mg kg(-1) D.W.) Fe > Zn > Pb > Ni > Cu > Cd. The levels of heavy metals varied significantly among fish species and tissues. Heavy metal levels were found generally higher in the liver and gills than the muscle in all species. The liver was the target organ for Cd, Cu, Fe, Ni, and Zn accumulation. Nickel and lead, however, exhibited their highest concentrations in the gills. The three studied fishes showed a difference in metals accumulation decreasing in following order S. vulgaris > D. annularis > L. aurata. Solea vulgaris with the highest TFwater, TFsediment, and metal concentrations in tissues would be considered as a potential bio-indicator in the south coast of Sfax for the assessment of environmental pollution status. Comparative studies with Luza zone indicate considerable bioaccumulation of heavy metals (Pb and Zn) in the various tissues of fish samples of the south coast of Sfax. PMID:27040537

  13. Prebiotics effect on immune and hepatic oxidative status and gut morphology of white sea bream (Diplodus sargus).

    Science.gov (United States)

    Guerreiro, Inês; Couto, Ana; Machado, Marina; Castro, Carolina; Pousão-Ferreira, Pedro; Oliva-Teles, Aires; Enes, Paula

    2016-03-01

    The aim of this study was to evaluate the effects of short-chain fructooligosaccharides (scFOS), xylooligosaccharides (XOS) and galactooligosaccharides (GOS) on immune and hepatic oxidative status, and gut morphology of white sea bream juveniles. Four diets were formulated: a control diet with fish meal (FM) and plant feedstuffs (PF) (30FM:70PF) and three test diets similar to the control but supplemented with 1% of scFOS, XOS or GOS. Dietary prebiotic incorporation did not affect total blood cell counts, hematocrit, hemoglobin, red blood indices or differential white blood cell counts. Fish fed GOS had lower ACH50 and nitric oxide than fish fed control diet. XOS enhanced immune status through the increase in alternative complement pathway (ACH50), lysozyme and total immunoglobulin. The higher activity of glucose 6-phosphate dehydrogenase in fish fed FOS compared to the other dietary groups was the only related antioxidant enzyme affected by prebiotics in the liver. GOS ameliorated the precocious adverse effects of PF based diet on gut histomorphology, as denoted by the lower incidence of histological alterations in fish fed GOS for 15 days. In conclusion, XOS and GOS at 1% might have potential to be used as prebiotics in white sea bream juveniles. PMID:26802896

  14. Mercury levels and trends (1993-2009) in bream (Abramis brama L.) and zebra mussels (Dreissena polymorpha) from German surface waters.

    Science.gov (United States)

    Lepom, Peter; Irmer, Ulrich; Wellmitz, Jörg

    2012-01-01

    Mercury concentrations have been analysed in bream (Abramis brama L.) and zebra mussels (Dreissena polymorpha) collected at 17 freshwater sites in Germany from 1993-2009 and 1994-2009, respectively, within the German Environmental Specimen programme. Mercury concentrations in bream ranged from 21 to 881 ng g(-1) wet weight with lowest concentrations found at the reference site Lake Belau and highest in fish from the river Elbe and its tributaries. Statistical analysis revealed site-specific differences and significant decreasing temporal trends in mercury concentrations at most of the sampling sites. The decrease in mercury levels in bream was most pronounced in fish from the river Elbe and its tributary Mulde, while in fish from the river Saale mercury levels increased. Temporal trends seem to level off in recent years. Mercury concentrations in zebra mussels were much lower than those in bream according to their lower trophic position and varied by one order of magnitude from 4.1 to 42 ng g(-1) wet weight (33-336 ng g(-1) dry weight). For zebra mussels, trend analyses were performed for seven sampling sites at the rivers Saar and Elbe of which three showed significant downward trends. There was a significant correlation of the geometric mean concentrations in bream and zebra mussel over the entire study period at each sampling site (Pearson's correlation coefficient=0.892, p=0.00002). A comparison of the concentrations in bream with the environmental quality standard (EQS) of 20 ng g(-1) wet weight set for mercury in biota by the EU showed that not a single result was in compliance with this limit value, not even those from the reference site. Current mercury levels in bream from German rivers exceed the EQS by a factor 4.5-20. Thus, piscivorous top predators are still at risk of secondary poisoning by mercury exposure via the food chain. It was suggested focusing monitoring of mercury in forage fish (trophic level 3 or 4) for compliance checking with the EQS for

  15. Dynamics of the content of H2O, Na, K, Ca and Mg in the eggs of bream,Abramis brama L. in natural conditions and under stress

    Institute of Scientific and Technical Information of China (English)

    Vladimir Ivanovich Martemyanov

    2016-01-01

    Objective:To study the dynamics of H2O, Na, K, Ca and Mg content in the oocytes of bream, Abramis brama observed in prespawning period on breeding ground and in reply to catching, transportation and subsequent remaining of spawners in a cage. Methods: For research on the dynamics of H2O and cations content in the oocytes of bream females,Abramis brama in the course of eggs transition from maturity Stage IV to Stage V, fishes were caught from breeding ground in the coastal zone of the Volga Reach of the Rybinsk reservoir at the Vereteya Station. For studying the influence of stress, the bream (76 individuals) were caught from breeding ground by hauling the seine during 15 min. Capture, sorting and transportation for 3 h to the ponds were the stress factors. Samples of oocytes from 6–8 fishes were taken immediately after capturing, then two more were taken during transportation. Later fishes were removed from the cage in certain time intervals. Concentration of Na and K in the dissolved samples of oocytes was measured by the spectrometer (Flapho-4, Carl Zeiss, Iena, Germany) and content of Ca and Mg was measured by atomic-absorption spectrometer-1 (the same producer). Results: In natural conditions before spawning in the course of maturation of oocytes from maturity Stage IV to V, H2O content in the ovicells of bream has increased by 3.3% and concentration of Na, K, Ca and Mg has decreased by 24.9%, 38.1%, 56.2% and 65.7%, accordingly. Stress caused by capturing, transportation and the subsequent remaining of bream spawners in a cage did not change parameters of water-salt exchange of the oocytes. Conclusions: In natural conditions before spawning, the maturation of oocytes of bream from maturity Stage IV to V take place. Stress caused by capturing, transportation and the subsequent remaining of bream spawners in a cage prevents the transition of eggs from maturity Stage IV to V. It is suggested that in order to develop optimal technique stimulating oocytes

  16. Dynamics of the content of H2O, Na, K, Ca and Mg in the eggs of bream, Abramis brama L. in natural conditions and under stress

    Directory of Open Access Journals (Sweden)

    Vladimir Ivanovich Martemyanov

    2016-04-01

    Full Text Available Objective: To study the dynamics of H2O, Na, K, Ca and Mg content in the oocytes of bream, Abramis brama observed in prespawning period on breeding ground and in reply to catching, transportation and subsequent remaining of spawners in a cage. Methods: For research on the dynamics of H2O and cations content in the oocytes of bream females, Abramis brama in the course of eggs transition from maturity Stage IV to Stage V, fishes were caught from breeding ground in the coastal zone of the Volga Reach of the Rybinsk reservoir at the Vereteya Station. For studying the influence of stress, the bream (76 individuals were caught from breeding ground by hauling the seine during 15 min. Capture, sorting and transportation for 3 h to the ponds were the stress factors. Samples of oocytes from 6–8 fishes were taken immediately after capturing, then two more were taken during transportation. Later fishes were removed from the cage in certain time intervals. Concentration of Na and K in the dissolved samples of oocytes was measured by the spectrometer (Flapho-4, Carl Zeiss, Iena, Germany and content of Ca and Mg was measured by atomic-absorption spectrometer-1 (the same producer. Results: In natural conditions before spawning in the course of maturation of oocytes from maturity Stage IV to V, H2O content in the ovicells of bream has increased by 3.3% and concentration of Na, K, Ca and Mg has decreased by 24.9%, 38.1%, 56.2% and 65.7%, accordingly. Stress caused by capturing, transportation and the subsequent remaining of bream spawners in a cage did not change parameters of water-salt exchange of the oocytes. Conclusions: In natural conditions before spawning, the maturation of oocytes of bream from maturity Stage IV to V take place. Stress caused by capturing, transportation and the subsequent remaining of bream spawners in a cage prevents the transition of eggs from maturity Stage IV to V. It is suggested that in order to develop optimal technique

  17. Genetic diversity of and differentiation among five populations of blunt snout bream (Megalobrama amblycephala revealed by SRAP markers: implications for conservation and management.

    Directory of Open Access Journals (Sweden)

    Wei Ji

    Full Text Available The blunt snout bream (Megalobrama amblycephala is an important freshwater aquaculture fish throughout China. Because of widespread introductions of this species to many regions, the genetic diversity of wild and natural populations is now threatened. In the present study, SRAP (sequence-related amplified polymorphism markers were used to assess genetic diversity of blunt snout bream. Three natural populations (Liangzi Lake, Poyang Lake and Yuni Lake, one cultured population (Nanxian and one genetic strain ('Pujiang No. 1' of blunt snout bream were screened with 88 SRAP primer combinations, of which 13 primer pairs produced stable and reproducible amplification patterns. In total, 172 bands were produced, of which 132 bands were polymorphic. Nei's gene diversity (h and Shannon's information index (I values provided evidence of differences in genetic diversity among the five populations (Poyang Lake>Liangzi Lake>Nanxian>'Pujiang No. 1'>Yuni Lake. Based on cluster analysis conducted on genetic distance values, the five blunt snout bream populations were divided into three groups, Poyang Lake and Liangzi Lake (natural populations, Nanxian and 'Pujiang No. 1' (cultured population and genetically selected strain, and Yuni Lake (natural population. Significant genetic differentiation was found among the five populations using analysis of molecular variance (AMOVA, with more genetic divergence existing among populations (55.49%, than within populations (44.51%. This molecular marker technique is a simple and efficient method to quantify genetic diversity within and among fish populations, and is employed here to help manage and conserve germplasm variability of blunt snout bream and to support the ongoing selective breeding programme for this fish.

  18. Development temperature has persistent effects on muscle growth responses in gilthead sea bream.

    Directory of Open Access Journals (Sweden)

    Daniel Garcia de la serrana

    Full Text Available Initially we characterised growth responses to altered nutritional input at the transcriptional and tissue levels in the fast skeletal muscle of juvenile gilthead sea bream. Fish reared at 21-22°C (range were fed a commercial diet at 3% body mass d(-1 (non-satiation feeding, NSF for 4 weeks, fasted for 4d (F and then fed to satiation (SF for 21d. 13 out of 34 genes investigated showed consistent patterns of regulation between nutritional states. Fasting was associated with a 20-fold increase in MAFbx, and a 5-fold increase in Six1 and WASp expression, which returned to NSF levels within 16h of SF. Refeeding to satiation was associated with a rapid (<24 h 12 to 17-fold increase in UNC45, Hsp70 and Hsp90α transcripts coding for molecular chaperones associated with unfolded protein response pathways. The growth factors FGF6 and IGF1 increased 6.0 and 4.5-fold within 16 h and 24 h of refeeding respectively. The average growth in diameter of fast muscle fibres was checked with fasting and significant fibre hypertrophy was only observed after 13d and 21d SF. To investigate developmental plasticity in growth responses we used the same experimental protocol with fish reared at either 17.5-18.5°C (range (LT or 21-22°C (range (HT to metamorphosis and then transferred to 21-22°C. There were persistent effects of development temperature on muscle growth patterns with 20% more fibres of lower average diameter in LT than HT group of similar body size. Altering the nutritional input to the muscle to stimulate growth revealed cryptic changes in the expression of UNC45 and Hsp90α with higher transcript abundance in the LT than HT groups, whereas there were no differences in the expression of MAFbx and Six1. It was concluded that myogenesis and gene expression patterns during growth are not fixed, but can be modified by temperature during the early stages of the life cycle.

  19. Pronounced population genetic differentiation in the rock bream Oplegnathus fasciatus inferred from mitochondrial DNA sequences.

    Science.gov (United States)

    Xiao, Yongshuang; Li, Jun; Ren, Guijing; Ma, Daoyuan; Wang, Yanfeng; Xiao, ZhiZhong; Xu, Shihong

    2016-05-01

    The population genetic structure of the rock bream (Oplegnathus fasciatus) along the coastal waters of China was estimated based on three mtDNA fragments (D-loop, COI, and Cytb). A total of 112 polymorphic sites were checked, which defined 63 haplotypes. A pattern with high levels of haplotype diversity (hCOI = 0.886 ± 0.034, hCytb = 0.874 ± 0.023) and low levels of nucleotide diversity (лCOI = 0.009 ± 0.005, лCytb = 0.006 ± 0.003) was detected based on the COI and Cytb fragments, and high levels of genetic diversity (hD-loop = 0.995 ± 0.007, лD-loop = 0.021 ± 0.011) were detected from the mtDNA D-loop. The population genetic diversity of O. fasciatus in south China was significantly higher than those of north China. Three genealogical clades were checked in the O. fasciatus populations based on the NJ and MST analyses of mtDNA COI gene sequence, and the genetic distances among the clades ranged from 0.018 to 0.025. Significant population genetic differentiation was also checked based on the Fst (0.331, p = 0.000) and exact p (0.000) test analyses. No significant population differentiations were checked based on mtDNA D-loop and Cytb fragments. Using a variety of phylogenetic methods, coalescent reasoning, and molecular dating interpreted in conjunction with paleoclimatic and physiographic evidences, we inferred that the genetic make-up of extant populations of O. fasciatus was shaped by Pleistocene environmental impacts on the historical demography of this species. Coalescent analyses (neutrality tests, mismatch distribution analysis, and Bayesian skyline analyses) showed that the species along coastline of China has experienced population expansions originated in its most recent history at about 169-175 kya before present. PMID:25427804

  20. Proximate Composition, Microbiological Quality and Sensory Attributes of Mahi-mahi (Coryphaena hippurus and Emperor Sea Bream (Lethrinus spp. Fillets Sold on Retail Market

    Directory of Open Access Journals (Sweden)

    Cătălina Nicoleta Boițeanu

    2014-11-01

    Full Text Available Introduction: The variety of seafood on the European market has considerably increased in recent years. This study presents data to the main nutritional values and the sensory properties of two exotic fish species, mahi-mahi (Coryphaena hippurus and emperor sea bream (Lethrinus spp., sold on retail market in Germany. Information on the quality of frozen and glazed fillets is still missing, but is important to characterize these products. This also includes details on the substances added to increase the water-binding ability. Aims: The paper aims to assess the quality of exotic fish fillets sold on the German market, through the evaluation of physical and chemical parameters, microbiological quality and sensory attributes. Materials and methods: 10 samples of each fish species were analysed after homogenisation to determine the following parameters:  pH-value (with a pH meter; water (gravimetrically; ash (in  a muffle furnace at 550 °C; salt (NaCl; by auto-titration; fat (by a modified Smedes method; protein (with a LECO TruSpecN based on the principles of the Dumas combustion method; total phosphorus content (photometrically; total volatile basic nitrogen (TVB-N, using the EU reference method; total viable bacteria count (TVC and specific fish spoiling bacteria (SSO (by decimal dilutions method. The sensory assessments were done with cooked and fried fillets by a panel consisting of 6 specialists, using a descriptive method. Results: Lipid, ash and salt contents of mahi-mahi were comparable to the emperor sea bream values. Due to low lipid content, both species can be classified as lean species. The protein amount of emperor sea bream was significantly higher compared to mahi-mahi and many other common fish species. In mahi-mahi samples, slightly higher values of total phosphates content were found. The pH values of emperor sea bream were in a normal range for fresh fish, whereas in mahi-mahi significantly higher values were determined which

  1. Bioremediation using Gracilaria chouae co-cultured with Sparus macrocephalus to manage the nitrogen and phosphorous balance in an IMTA system in Xiangshan Bay, China.

    Science.gov (United States)

    Wu, Hailong; Huo, Yuanzi; Han, Fang; Liu, Yuanyuan; He, Peimin

    2015-02-15

    A cage experiment using the red alga Gracilaria chouae co-cultured with the black seabream Sparus macrocephalus in Xiangshan Bay, China was conducted to measure the nutrient flux of the integrated multi-trophic aquaculture (IMTA) system. Results showed that trash fish were the main nutrient input contributor and adult fish were the main nutrient output contributor in the system. Contents of N and P in adult fish accounted for 54.45% and 59.48% of N and P in trash fish and fry, which suggests that 45.55% of N and 40.52% of P generated by fish farming were released into to the water. G. chouae proved to be an efficient bioremediation species in this IMTA system. To balance the excess nutrients generated by the system, 231.09 kg of seedlings should be cultured and 5315.07 kg of adult seaweed should be harvested. PMID:25561001

  2. Attractive effect of acoustic taming on Sparus macrocephalus in a cage%网箱中黑鲷音响驯化的诱集效果探究

    Institute of Scientific and Technical Information of China (English)

    陈德慧; 刘洪生; 胡庆松; 王伟定; 章守宇

    2012-01-01

    音响驯化作为鱼类行为控制技术之一,对海洋牧场内的鱼种行为控制有明显的作用。在驯化声源中,目标鱼种栖息海域背景噪声有望具有更佳的驯化效果。为此,在天然水域的网箱中采用录制的黑鲷栖息地背景噪声对黑鲷(Sparus macrocephalus)进行了音响驯化。由现场观察及数据分析,自然海域声音配合投饵能对黑鲷产生较好的集聚效果。黑鲷幼苗音响驯化一般经历3个过程,即环境适应,对饵料的需求达到一定量,建立声音与摄食之间的条件反射;黑鲷能识别声音,并维持一定的记忆时间。实验结果表明,采用海域背景噪声的音响驯化,配合投饵是一种有效的手段,并可以使黑鲷对声音的短期记忆转化为长期记忆。研究亮点:目前国内音响驯化研究环境主要停留在室内水槽中,自然水域网箱中的音响驯化国内未见报道。并且尝试将黑鲷放流海域的水下背景噪声作为驯化声源,探究鱼群诱集效果。另外将Atkinson-Shiffrin记忆理论运用到鱼类音响驯化领域中,探讨了定时声音刺激对黑鲷的行为反馈以及记忆变化的影响。%Fish acoustic taming, as one of the technology in the control of fish behavior, have good promotion in fish control in sea ranching. Among the sound sources, habitat waters background sound of target fishbased acoustic taming intends to involve better taming effect. To verify this, habitat waters' background sound of Sparus macrocephalus is utilized to conduct the acoustic taming in a cage. The results based on the analysis of data and participant experiment observation showed excellent aggregation effect. Acoustic taming on juvenile Sparus macrocephalus needs three stages : acclimatization ; impulsion of demanding food ( outside sign is that fish reaches certain length and weight) ; building conditioned reflex between sound and bait. Sparus macrocephalus can identify different sounds and

  3. GROWTH PROMOTION OF RED SEA BREAM, PAGROSOMUS MAJOR, BY ORAL ADMINISTRATION OF RECOMBINANT EEL AND SALMON GROWTH HORMONE

    Institute of Scientific and Technical Information of China (English)

    徐迎利; 苗宏志; 刘振辉; 邓勇; 兰山; 王尧; 张培军; 徐斌; 麦康森

    2001-01-01

    Recombinant eel GH and yeast containing chinook salmon growth hormone (reGHand rcsGH) were incorporated into gelatin and sodium alginate (reGH-GS and rcsGH-GS) or polymer ma-trix (reGH-HP55) to protect the hormone from proteolytic cleavage in the stomach. The diets containin greGH-GS, rcsGH-GS, reGH-HP55 and free-reGH or uncoated-rcsGH were administered to red sea bream. Feeding of reGH-GS, reGH-HP55 and rcsGH-GS diets resulted in significant increases in body weight and fork length over those of controls. These results strongly suggest that gelatin and sodium algi-nate as well as polymer matrix protected the hormone from proteolytic enzymes in the gastrointestinal tract to allow the bioactive hormone to enter the circulation and eventually stimulato fish growth.

  4. GROWTH PROMOTION OF RED SEA BREAM, PAGROSOMUS MAJOR, BY ORAL ADMINISTRATION OF RECOMBINANT EEL AND SALMON GROWTH HORMONE

    Institute of Scientific and Technical Information of China (English)

    2001-01-01

    Recombinant eel GH and yeast containing chinook salmon growth hormone (reGH and rcsGH) were incorporated into gelatin and sodium alginate (reGH-GS and rcsGH-GS) or polymer matrix (reGH-HP55) to protect the hormone from proteolytic cleavage in the stomach. The diets containing reGH-GS, rcsGH-GS, reGH-HP55 and free-reGH or uncoated-rcsGH were administered to red sea bream. Feeding of reGH-GS, reGH-HP55 and rcsGH-GS diets resulted in significant increases in body weight and fork length over those of controls. These results strongly suggest that gelatin and sodium alginate as well as polymer matrix protected the hormone from proteolytic enzymes in the gastrointestinal tract to allow the bioactive hormone to enter the circulation and eventually stimulate fish growth.

  5. Purification and characterization of a collagenolytic serine proteinase from the skeletal muscle of red sea bream (Pagrus major).

    Science.gov (United States)

    Wu, Guo-Ping; Chen, Su-Hua; Liu, Guang-Ming; Yoshida, Asami; Zhang, Ling-Jing; Su, Wen-Jin; Cao, Min-Jie

    2010-03-01

    A collagenolytic serine proteinase (CSP) was purified from red sea bream (Pagrus major) skeletal muscle to homogeneity by ammonium sulfate fractionation and chromatographies including DEAE-Sephacel, Phenyl Sepharose and Hydroxyapatite. The molecular mass of CSP was approximately 85 kDa as estimated by SDS-PAGE and gel filtration. Optimum temperature and pH of CSP were 40 degrees C and 8.0, respectively. CSP was specifically inhibited by serine proteinase inhibitors, while inhibitors to other type proteinases did not show much inhibitory effects. The K(m) and k(cat) values of CSP for Boc-Leu-Lys-Arg-MCA were 3.58 microM and 0.13 s(-1) at 37 degrees C, respectively. Furthermore, CSP hydrolyzed gelatin and native type I collagen effectively though its degradation on myosin heavy chain (MHC) was not significant, suggesting its involvement in the texture tenderization of fish muscle during the post-mortem stage. PMID:19945542

  6. Growth promotion of red sea bream, Pagrosomus major, by oral administration of recombinant eel and salmon growth hormone

    Science.gov (United States)

    Xu, Bin; Mai, Kang-Sen; Xu, Ying-Li; Miao, Hong-Zhi; Liu, Zhen-Hui; Dong, Yong; Lan, Shan; Wang, Rao; Zhang, Pei-Jun

    2001-06-01

    Recombinant eel GH and yeast containing chinook salmon growth hormone (reGH and rcsGH) were incorporated into gelatin and sodium alginate (reGH-GS and rcsGH-GS) or polymer matrix (reGH-HP55) to protect the hormone from proteolytic cleavage in the stomach. The diets containing reGH-GS, rcsGH-GS, reGH-HP55 and free-reGH or uncoated-rcsGH were administered to red sea bream. Feeding of reGH-GS, reGH-HP55 and rcsGH-GS diets resulted in significant increases in body weight and fork length over those of controls. These results strongly suggest that gelatin and sodium alginate as well as polymer matrix protected the hormone from proteolytic enzymes in the gastrointestinal tract to allow the bioactive hormone to enter the circulation and eventually stimulate fish growth.

  7. Molecular identification and functional characterisation of the interferon regulatory factor 1 in the blunt snout bream (Megalobrama amblycephala).

    Science.gov (United States)

    Zhan, Fan-Bin; Liu, Han; Lai, Rui-Fang; Jakovlić, Ivan; Wang, Wen-Bin; Wang, Wei-Min

    2016-07-01

    Interferon regulatory factors (IRFs) play a key role in mediating the host response against pathogen infection and other important biological processes. This is the first report of an IRF family member in blunt snout bream Megalobrama amblycephala. The complete cDNA of M. amblycephala (Ma) IRF1 gene has 1422 nucleotides (nt.), with an open reading frame of 858 nt, encoding a polypeptide of 285 amino acids. The putative MaIRF1 polypeptide shared significant structural homology with known IRF1 homologs: a conserved IRF domain was found at the N-terminal and an IRF association domain 2 at the C-terminal. Phylogenetic analysis showed that MaIRF1 amino acid sequence clustered with other teleost IRF1s, with a grass carp ortholog exhibiting the highest similarity. MaIRF1 mRNA expression patterns were studied using quantitative real-time PCR in healthy fish tissues and after a challenge with Aeromonas hydrophila bacterium. It was constitutively expressed in all examined tissues: the highest in blood, the lowest in muscle. The expression after A. hydrophila challenge was up-regulated in liver, spleen and kidney, but down-regulated in intestine and gills. At the protein level, similar expression patterns were observed in liver and gills. Patterns differed in intestine (up-regulation), spleen (down-regulation) and kidney (expression mostly unchanged). This study indicates that MaIRF1 gene plays an important role in the blunt snout bream immune system, hence providing an important base for further studies. PMID:27150048

  8. Chlorinated hydrocarbons- (CHC) and PCDD/F-levels in sediments and breams (Abramis brama) from the river Elbe (contribution to the German Environmental Specimen Banking)

    Energy Technology Data Exchange (ETDEWEB)

    Oxynos, K. [GSF-Forschungszentrum fuer Umwelt und Gesundheit, Institut fuer Oekologische Chemie, Oberschleissheim (Germany); Schramm, K.W. [GSF-Forschungszentrum fuer Umwelt und Gesundheit, Institut fuer Oekologische Chemie, Oberschleissheim (Germany); Marth, P. [GSF-Forschungszentrum fuer Umwelt und Gesundheit, Institut fuer Oekologische Chemie, Oberschleissheim (Germany); Schmitzer, J. [GSF-Forschungszentrum fuer Umwelt und Gesundheit, Institut fuer Oekologische Chemie, Oberschleissheim (Germany); Kettrup, A. [GSF-Forschungszentrum fuer Umwelt und Gesundheit, Institut fuer Oekologische Chemie, Oberschleissheim (Germany)

    1995-09-01

    Chlorinated hydrocarbons have been determined in sediments and breams (Abramis brama) from different locations along the river Elbe, starting from the border to the Czech Republic down-stream up to Cumlosen (river km 470), near the frontier of the former German Democratic Republic. High levels of hexachlorobenzene (HCB) have been found in sediments. HCB, DDT-metabolites and octachlorostyrene (OCS) have been the most dominant compounds in bream, especially fish from eastern sampling sites have been heavily contaminated. Furthermore, sediments from 1991-1993 have been analysed to determine polychlorinated dibenzo-p-dioxins and dibenzofurans (PCDD/F). The CHC-burden of the river Elbe declines downstream, whereas the PCDD/F-content increases in that direction. (orig.)

  9. Complement factor D homolog involved in the alternative complement pathway of rock bream (Oplegnathus fasciatus): Molecular and functional characterization and immune responsive mRNA expression analysis.

    Science.gov (United States)

    Godahewa, G I; Perera, N C N; Bathige, S D N K; Nam, Bo-Hye; Noh, Jae Koo; Lee, Jehee

    2016-08-01

    The complement system serves conventional role in the innate defense against common invading pathogens. Complement factor D (CfD) is vital to alternative complement pathway activation in cleaving complement factor B. This catalytic reaction forms the alternative C3 convertase that is crucial for complement-mediated pathogenesis. In this study, rock bream (Oplegnathus fasciatus) CfD (OfCfD) was characterized and OfCfD mRNA expression was investigated. OfCfD encodes 277 amino acids (aa) for a 30-kDa polypeptide. A domain analysis of the deduced OfCfD aa sequence showed a single serine protease trypsin superfamily domain, a serine active region, three active sites, and three substrate-binding sites. Pairwise sequence comparisons indicated that OfCfD has the highest identity (84.5%) with Oreochromis niloticus CfD. The phylogenetic tree revealed a common ancestral origin of CfD members, with fish CfD distinct from other vertebrate orthologs. The structural arrangement of the OfCfD gene (2451 bp) contained five exons interrupted by four introns. A spatial transcriptional analysis indicated that OfCfD transcripts constitutively expressed in all of the examined rock bream tissues, and that they were highest in the spleen and liver. In addition, OfCfD transcripts were immunologically upregulated by lipopolysaccharide (LPS) (12 h p.i.), Streptococcus iniae (12 h p.i.), rock bream iridovirus (RBIV) (6-12 h p.i.), and poly I:C (6 h p.i.) in spleen tissue. OfCfD is a trypsin protease and its recombinant protein showed strong protease activity similar to that of trypsin, indicating its catalytic function in the alternative pathway. Together, our findings suggest that OfCfD might be involved in immune responses in rock bream. PMID:27311435

  10. Ocean warming alters cellular metabolism and induces mortality in fish early life stages: A proteomic approach.

    Science.gov (United States)

    Madeira, D; Araújo, J E; Vitorino, R; Capelo, J L; Vinagre, C; Diniz, M S

    2016-07-01

    Climate change has pervasive effects on marine ecosystems, altering biodiversity patterns, abundance and distribution of species, biological interactions, phenology, and organisms' physiology, performance and fitness. Fish early life stages have narrow thermal windows and are thus more vulnerable to further changes in water temperature. The aim of this study was to address the sensitivity and underlying molecular changes of larvae of a key fisheries species, the sea bream Sparus aurata, towards ocean warming. Larvae were exposed to three temperatures: 18°C (control), 24°C (warm) and 30°C (heat wave) for seven days. At the end of the assay, i) survival curves were plotted for each temperature treatment and ii) entire larvae were collected for proteomic analysis via 2D gel electrophoresis, image analysis and mass spectrometry. Survival decreased with increasing temperature, with no larvae surviving at 30°C. Therefore, proteomic analysis was only carried out for 18°C and 24°C. Larvae up-regulated protein folding and degradation, cytoskeletal re-organization, transcriptional regulation and the growth hormone while mostly down-regulating cargo transporting and porphyrin metabolism upon exposure to heat stress. No changes were detected in proteins related to energetic metabolism suggesting that larval fish may not have the energetic plasticity needed to sustain cellular protection in the long-term. These results indicate that despite proteome modulation, S. aurata larvae do not seem able to fully acclimate to higher temperatures as shown by the low survival rates. Consequently, elevated temperatures seem to have bottleneck effects during fish early life stages, and future ocean warming can potentially compromise recruitment's success of key fisheries species. PMID:27062348

  11. Caspase-1 and IL-1β processing in a teleost fish.

    Directory of Open Access Journals (Sweden)

    Marta I R Reis

    Full Text Available Interleukine-1β (IL-1β is the most studied pro-inflammatory cytokine, playing a central role in the generation of systemic and local responses to infection, injury, and immunological challenges. In mammals, IL-1β is synthesized as an inactive 31 kDa precursor that is cleaved by caspase-1 generating a 17.5 kDa secreted active mature form. The caspase-1 cleavage site strictly conserved in all mammalian IL-1β sequences is absent in IL-1β sequences reported for non-mammalian vertebrates. Recently, fish caspase-1 orthologues have been identified in sea bass (Dicentrarchus labrax and sea bream (Sparus aurata but very little is known regarding their processing and activity. In this work it is shown that sea bass caspase-1 auto-processing is similar to that of the human enzyme, resulting in active p24/p10 and p20/p10 heterodimers. Moreover, the presence of alternatively spliced variants of caspase-1 in sea bass is reported. The existence of caspase-1 isoforms in fish and in mammals suggests that they have been evolutionarily maintained and therefore are likely to play a regulatory role in the inflammatory response, as shown for other caspases. Finally, it is shown that sea bass and avian IL-1β are specifically cleaved by caspase-1 at different but phylogenetically conserved aspartates, distinct from the cleavage site of mammalian IL-1β.

  12. PHYTOPLANKTON ASSEMBLAGES AT FISH FARM IN MASLINOVA BAY (THE ISLAND OF BRAČ

    Directory of Open Access Journals (Sweden)

    Sanda Skejić

    2012-07-01

    Full Text Available The aim of this study was to establish phytoplankton composition at the sea bream (Sparus aurata and sea bass (Dicentrarchus labrax fish farm in the middle Adriatic Sea. The investigation was performed from September 2005 to September 2006 at a station located in Maslinova Bay at the island of Brač. Considering the whole research period, diatoms generally prevailed in terms of abundance while dinoflagellates were particularly abundant in June. Number of species of diatoms in comparison to dinoflagellates through the investigated period was similar. From 111 species of phytoplankton found, there were 55 species of Bacillariophyceae (diatoms, 47 species of Dinophyta (dinoflagellates, 5 species of Prymnesiophyceae, 3 Chrysophyceae and 1 Euglenophyta. Among the diatoms, the majority of species belonged to genus Chaetoceros. The most represented dinoflagellate genera were Oxytoxum and Gymnodinium. There were no considerable differences in phytoplankton composition with respect to different depths, but seasonal influence was significant. Biodiversity and abundance ranges of phytoplankton species indicated good water conditions and there were no evident alterations induced by the increased release of nutrients.

  13. Applications of self-organizing maps for ecomorphological investigations through early ontogeny of fish.

    Directory of Open Access Journals (Sweden)

    Tommaso Russo

    Full Text Available We propose a new graphical approach to the analysis of multi-temporal morphological and ecological data concerning the life history of fish, which can typically serves models in ecomorphological investigations because they often undergo significant ontogenetic changes. These changes can be very complex and difficult to describe, so that visualization, abstraction and interpretation of the underlying relationships are often impeded. Therefore, classic ecomorphological analyses of covariation between morphology and ecology, performed by means of multivariate techniques, may result in non-exhaustive models. The Self Organizing map (SOM is a new, effective approach for pursuing this aim. In this paper, lateral outlines of larval stages of gilthead sea bream (Sparus aurata and dusky grouper (Epinephelus marginatus were recorded and broken down using by means of Elliptic Fourier Analysis (EFA. Gut contents of the same specimens were also collected and analyzed. Then, shape and trophic habits data were examined by SOM, which allows both a powerful visualization of shape changes and an easy comparison with trophic habit data, via their superimposition onto the trained SOM. Thus, the SOM provides a direct visual approach for matching morphological and ecological changes during fish ontogenesis. This method could be used as a tool to extract and investigate relationships between shape and other sinecological or environmental variables, which cannot be taken into account simultaneously using conventional statistical methods.

  14. Levels of 210Po in some commercial fish species consumed in the Aegean Sea coast of Turkey and the related dose assessment to the coastal population

    International Nuclear Information System (INIS)

    Concentrations of 210Po were determined in the edible muscle tissue of five species of marine fish: Sardine (Sardine plichardus) and Anchovy (Engraulis encrasicolus), Red mullet (Mullus barbatus), Horse mackerel (Trachurus trachurus) and Gilt-head sea bream (Sparus aurata) collected from five stations in the Aegean Sea Region of Turkey during the fishery season 2010. The 210Po concentrations in the fish samples were found to vary from not detected levels to 389 ± 45 Bq kg−1 dry wt. These variations in 210Po content in different species are probably due to differences in metabolism and feeding patterns. The highest levels for 210Po were observed in Anchovy (E. encrasicolus) species. -- Highlights: ► This study was determined the 210Po levels in the fish. ► Different fish species were collected in the Turkish coast of Aegean Sea. ► Higher 210Po concentrations were found in Anchovy. ► The annual dose from the consumption of the fish samples was calculated. ► The ingestion dose was found in the range 0.041–14.66 μSv y−1

  15. Comparison of heavy metal concentration of some marine fishes from Black and Aegean Seas

    Directory of Open Access Journals (Sweden)

    Makedonski Lubomir

    2015-06-01

    Full Text Available Major part of healthy human diet consist of marine fish and seafood products. And it is not surprising that there are numerous studies based on metal accumulation in various fish species. Fish may also be used for heavy metal monitoring programs of marine environments due to their easy sampling, sample preparation and chemical analysis. Concentrations of lead, cadmium, nickel, copper, manganese, zinc, iron, chromium, total mercury and total arsenic were determined in edible part of two commercially valuable fish Greek aquaculture species European sea bass (Dicentrarchus labrax and gilthead sea bream (Sparus aurata purchased from Bulgarian market during 2011. The concentration of metals was measured by atomic absorption spectrophotometry (AAS. The concentration of the heavy metals in examined fish species ranged as follow: Pb 0.008 - 0.013; Cd 0.0017 - 0.022; Ni 0.007 - 0.012; Cu 0.054 - 0.115; Mn 0.043 - 0.09; Zn 0.14 - 0.15; Fe 0.17 - 0.19; Cr 0.05 - 0.07; Hg 0.11 - 0.13; As 1.6 - 1.8 mg kg-1 wet weight, respectively.

  16. Culture of marine sponges with potential applications in Aquaculture and Biotechnology

    Directory of Open Access Journals (Sweden)

    Susana M F Ferreira

    2014-06-01

    In this study, the sponges were collected from the sea bottom in the surrounding areas of Peniche (central western coast of Portugal, by scuba diving. They were sealed in plastic zip bags during transportation to the surface and laboratory, to avoid air contact, which they are not able to endure. They were maintained in a closed water circulation system, transplanted into artificial substrates of plastic and fed every two days, with a mixed solution of microalgae Nanochloropsis salina culture and faeces of gilt-head sea bream (Sparus aurata. As this species is susceptible to the light, half of the tanks of the culture system were protected with a black cloth and the other half were submitted to an acclimation process to this factor, in order for them to be used in aquariophilia. The sponges dimension and weight were assessed. The establishment of an efficient culture strategy will allow to use sponges as ornamental organism, as well as a diet for other interesting commercial species (also potentially as probiotics, or even as source of extracts for different biotechnology fields.

  17. Dinàmica deIs nutrients midó-C(13) i prote'ina-NI(5) en la truita irisada (Oncorhynchus mykiss) i l'orada (Sparus aurata): efectes de la gelatinització deIs carbohidrats, la natació sostinguda i la ritmicitat

    OpenAIRE

    Felip Arias, Olga Mª

    2013-01-01

    [cat] L’èxit de l’aqüicultura moderna es basa en el control sobre la reproducció de les espècies, en el millor coneixement de la seva biologia, en les innovacions tecnològiques i en el desenvolupament d’aliments específics. En base a aquests requisits per la millora de la pràctica de l’aqüicultura, i també a l'esmenada necessitat de fonts alternatives de les matèries primeres (farina i oli de peix) per als pinsos d’engreix, es van plantejar els estudis que constitueixen la present tesis...

  18. Dinàmica deIs nutrients midó-C(13) i prote'ina-NI(5) en la truita irisada (Oncorhynchus mykiss) i l'orada (Sparus aurata): efectes de la gelatinització deIs carbohidrats, la natació sostinguda i la ritmicitat

    OpenAIRE

    Felip Arias, Olga Mª

    2013-01-01

    L’èxit de l’aqüicultura moderna es basa en el control sobre la reproducció de les espècies, en el millor coneixement de la seva biologia, en les innovacions tecnològiques i en el desenvolupament d’aliments específics. En base a aquests requisits per la millora de la pràctica de l’aqüicultura, i també a l'esmenada necessitat de fonts alternatives de les matèries primeres (farina i oli de peix) per als pinsos d’engreix, es van plantejar els estudis que constitueixen la present tesis. Els trebal...

  19. Purification and characterization of a gelatinolytic metalloproteinase from the skeletal muscle of red sea bream (Pagrus major).

    Science.gov (United States)

    Wu, Guo-Ping; Cao, Min-Jie; Chen, Su-Hua; Weng, Wu-Yin; Cai, Qiu-Feng; Su, Wen-Jin

    2010-05-12

    A gelatinolytic metalloproteinase (gMP) from red sea bream ( Pagrus major ) skeletal muscle was highly purified by ammonium sulfate fractionation and column chromatographies including (diethylamino)ethyl (DEAE)-Sephacel, phenyl-Sepharose, and gelatin-Sepharose. Purified gMP revealed two bands with molecular masses of 52 and 55 kDa as estimated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) under reducing conditions. The 55 kDa band is quite possibly a glycosylated form of the 52 kDa band. The proteinase revealed optimal activity at 40 degrees C and pH 8.0. Metalloproteinase inhibitors including ethylenediaminetetraacetic acid (EDTA), ethylene glycol bis(2-aminoethyl ether)-N,N,N',N'-tetraacetic acid (EGTA), and 1,10-phenanthroline specifically suppressed its activity. gMP was also significantly inhibited by cysteine and dithiothreitol. Divalent metal ion Ca(2+) is essential for its gelatinolytic activity. Thus, the proteinase is regarded as a matrix metalloproteinase-like proteinase. Furthermore, gMP hydrolyzed gelatin and type-I collagen effectively even at 4 degrees C, suggesting the possibility of its involvement in the texture tenderization of fish muscle during the post-mortem stage. PMID:20384341

  20. Transcriptional variants of Dmrt1 and expression of four Dmrt genes in the blunt snout bream, Megalobrama amblycephala.

    Science.gov (United States)

    Su, Lina; Zhou, Fengjuan; Ding, Zhujin; Gao, Zexia; Wen, Jiufu; Wei, Wei; Wang, Qijun; Wang, Weimin; Liu, Hong

    2015-12-01

    Doublesex and Mab3 related transcription factor (DMRT), characterized by a conserved DM domain, function as sex-related transcription factors and also play critical roles in ontogenesis. In this study, 4 Dmrt genes in the blunt snout bream, Megalobrama amblycephala, were identified, characterized and their mRNA expression in different adult organs, during embryogenesis and gonadal development in larvae were determined by quantitative real time PCR. There are 4 Dmrt1 isoforms in the M. amblycephala genome, which were expressed highly in the testis and weakly in the ovary. The complete cDNAs of the M. amblycephala Dmrt2a, Dmrt2b and Dmrt3 were predicted to encode 510, 328 and 449 amino acids, respectively. The M. amblycephala Dmrt2a mRNA peaked at 11hpf (hour post fertilizing) during early embryonic stages, while Dmrt2b was highly expressed during late embryonic stages. Both the M. amblycephala Dmrt2a and Dmrt2b were expressed highly in the gill and exhibited a sexually dimorphic expression pattern. The M. amblycephala Dmrt3 was expressed highly in the gill, muscle and brain, at 40dph (day post hatching) during early development and at stage V in the testis during gonadal development. All fish Dmrts except Dmrt5 were found in the M. amblycephala genome. The observed expression patterns of these Dmrts in developing embryos and larvae, as well as different adult organs indicate conserved sexual or extragonadal functions of the Dmrts through evolution. PMID:26188158

  1. A Comprehensive Analysis of Codon Usage Patterns in Blunt Snout Bream (Megalobrama amblycephala Based on RNA-Seq Data

    Directory of Open Access Journals (Sweden)

    Xiaoke Duan

    2015-05-01

    Full Text Available Blunt snout bream (Megalobrama amblycephala is an important fish species for its delicacy and high economic value in China. Codon usage analysis could be helpful to understand its codon biology, mRNA translation and vertebrate evolution. Based on RNA-Seq data for M. amblycephala, high-frequency codons (CUG, AGA, GUG, CAG and GAG, as well as low-frequency ones (NUA and NCG codons were identified. A total of 724 high-frequency codon pairs were observed. Meanwhile, 14 preferred and 199 avoided neighboring codon pairs were also identified, but bias was almost not shown with one or more intervening codons inserted between the same pairs. Codon usage bias in the regions close to start and stop codons indicated apparent heterogeneity, which even occurs in the flanking nucleotide sequence. Codon usage bias (RSCU and SCUO was related to GC3 (GC content of 3rd nucleotide in codon bias. Six GO (Gene ontology categories and the number of methylation targets were influenced by GC3. Codon usage patterns comparison among 23 vertebrates showed species specificities by using GC contents, codon usage and codon context analysis. This work provided new insights into fish biology and new information for breeding projects.

  2. [RAPD fingerprinting of common bream Abramis brama L., roach Rutilus rutilus L., and their F1 hybrids].

    Science.gov (United States)

    Khrisanfova, G G; Ludannyĭ, R I; Slyn'ko, Iu V; Iakovlev, V N; Cemenova, S K

    2004-10-01

    The polymerase chain reaction with arbitrary primers (RAPD-PCR) was used to study and to evaluate the genetic variation in the hybrid progeny of two Cyprinidae species, common bream Abramis brama and roach Rutilus rutilus. Genetic polymorphism was studied in 20 fishes (young of the current year) obtained in four individual crosses: R. rutilus x R. rutilus (RR), A. brama x A. brama (AA), R. rutilus x A. brama (RA), and A. brama x R. rutilus (AR). Amplification spectra obtained with eight primers contained 288 fragments, 97.6% of which proved to be polymorphic. The proportion of polymorphic fragments was 75.0% in the RR progeny, 58.1% in the AA progeny, 84.9% in the AR progeny, and 77.8% in the RA progeny. Classification analysis in the space of principal components was performed with the first four components, which together accounted for 64% of the total variance of the character under study. The individual contributions of components I, II, III, and IV were 26.8, 16.8, 11.5, and 8.9%, respectively. Fishes of the two pure species and the hybrid progeny (direct and reverse hybrids together) were clearly differentiated in the space of principal components I and II. The best differentiation of the four samples (RR, AA, RA, and AR) was observed in the space of principal components II and IV. Possible causes of high genetic variation in interspecific hybrids are discussed. PMID:15575514

  3. Significant population genetic structure detected in the rock bream Oplegnathus fasciatus (Temminck & Schlegel, 1844) inferred from fluorescent-AFLP analysis

    Science.gov (United States)

    Xiao, Yongshuang; Ma, Daoyuan; Xu, Shihong; Liu, Qinghua; Wang, Yanfeng; Xiao, Zhizhong; Li, Jun

    2016-05-01

    Oplegnathus fasciatus (rock bream) is a commercial rocky reef fish species in East Asia that has been considered for aquaculture. We estimated the population genetic diversity and population structure of the species along the coastal waters of China using fluorescent-amplified fragment length polymorphisms technology. Using 53 individuals from three populations and four pairs of selective primers, we amplified 1 264 bands, 98.73% of which were polymorphic. The Zhoushan population showed the highest Nei's genetic diversity and Shannon genetic diversity. The results of analysis of molecular variance (AMOVA) showed that 59.55% of genetic variation existed among populations and 40.45% occurred within populations, which indicated that a significant population genetic structure existed in the species. The pairwise fixation index F st ranged from 0.20 to 0.63 and were significant after sequential Bonferroni correction. The topology of an unweighted pair group method with arithmetic mean tree showed two significant genealogical branches corresponding to the sampling locations of North and South China. The AMOVA and STRUCTURE analyses suggested that the O. fasciatus populations examined should comprise two stocks.

  4. Allometric growth in rock bream larvae (Oplegnathus fasciatus Temminck et Schlegel 1844)%条石鲷早期发育阶段的生长模式

    Institute of Scientific and Technical Information of China (English)

    何滔; 肖志忠; 刘清华; 李军

    2012-01-01

    The rock bream, Oplegnathus fasciatus, a subtropical and carnivorous species, is an economically important marine fish in East Asia. The high commercial and ornamental value makes it a promising aquaculture species in the future. However, to some extent, the lack of information on ontogenetic development has restricted the breeding industry of this species. In this study, the allometric growth in rock bream was analyzed. The results are as follows: at the general condition for fingerling-production, the total length and body weight of rock bream larvae were measured from hatching to 50 days post hatching. The increase of total length and body weight could be estimated with the Cubic function and took on the S-Curve. The curve could be divided into three phases and each phase possessed different growth rate. The head length, head height, trunk height, eye diameter, mouth width, rostrum length, abdomen length, and tail fin length of rock bream were measured and the relationship between them and the total length was analyzed. The result showed the allometric growth in rock bream larvae. By analyzing the inflexion points in growth curves, in combination with morphological development of the larvae, we found that some important organs (head, mouth, eye, digestive tract and fins) had developed prior to other ones. In rearing rock bream larvae, the best environmental condition should be established by making the important organs prior development.%为研究条石鲷在早期发育阶段的生长特性和重要功能器官的异速生长规律,测定了条石鲷仔稚幼鱼(0~50日龄)全长和体质量随日龄的生长变化. 通过统计学方法分析发现,全长、体质量随日龄的变化均符合Cubic函数关系式,其变化曲线呈S型.全长、体质量随日龄的变化可分为3个阶段,不同阶段的全长、体质量生长率具有显著性差异(P<0.05).运用SPSS 13.0和OriginPro 7.5软件分析了条石鲷仔稚幼鱼头长、头高、体高、

  5. Production and characterization of recombinantly derived peptides and antibodies for accurate determinations of somatolactin, growth hormone and insulin-like growth factor-I in European sea bass (Dicentrarchus labrax).

    Science.gov (United States)

    de Celis, S Vega-Rubín; Gómez-Requeni, P; Pérez-Sánchez, J

    2004-12-01

    A specific radioimmunoassay (RIA) for European sea bass (Dicentrarchus labrax) growth hormone (GH) was developed and validated. For this purpose, a stable source of GH was produced by means of recombinant DNA technology in a bacteria system. The identity of the purified protein (ion exchange chromatography) was demonstrated by Western blot and a specific GH antiserum was raised in rabbit. In Western blot and RIA system, this antiserum recognized specifically native and recombinant GH, and it did not cross-react with fish prolactin (PRL) and somatolactin (SL). In a similar way, a specific polyclonal antiserum against the now available recombinant European sea bass SL was raised and used in the RIA system to a sensitivity of 0.3 ng/ml (90% of binding of tracer). Further, European sea bass insulin-like growth factor-I (IGF-I) was cloned and sequenced, and its high degree of identity with IGF-I peptides of barramundi, tuna, and sparid fish allowed the use of a commercial IGF-I RIA based on barramundi IGF-I antiserum. These assay tools assisted for the first time accurate determinations of SL and GH-IGF-I axis activity in a fish species of the Moronidae family. Data values were compared to those found with gilthead sea bream (Sparus aurata), which is currently used as a Mediterranean fish model for growth endocrinology studies. As a characteristic feature, the average concentration year round of circulating GH in growing mature males of European sea bass was higher than in gilthead sea bream. By contrast, the average concentration of circulating SL was lower. Concerning to circulating concentration of IGF-I, the measured plasma values for a given growth rate were also lower in European sea bass. These findings are discussed on the basis of a different energy status that might allowed a reduced but more continuous growth in European sea bass. PMID:15560873

  6. Immune responses and stress resistance in red sea bream, Pagrus major, after oral administration of heat-killed Lactobacillus plantarum and vitamin C.

    Science.gov (United States)

    Dawood, Mahmoud A O; Koshio, Shunsuke; Ishikawa, Manabu; Yokoyama, Saichiro

    2016-07-01

    The present study evaluated the interactive benefits of dietary administration of heat-killed Lactobacillus plantarum (LP) and vitamin C (VC) on the growth, oxidative status and immune response of red sea bream (Pagrus major). A diet without LP and VC supplements was employed as a control diet. Four other test diets with 0 or 1 g LP kg(-1) combined with 0.5 or 1 g VC kg(-1) (2 × 2 factorial design) were fed to red sea bream (2 ± 0.01 g) for 56 days. A significant interaction was found between LP and VC on final body weight (FNW), weight gain (WG), hematocrit (HCT), serum bactericidal (BA) and lysozyme (LZY) activities, mucus LZY and peroxidase (PA) activities, nitro blue tetrazolium (NBT), catalase, mucus secretion and tolerance against low salinity stress test (LT50) (P < 0.05). In addition, FNW, WG, specific growth rate, feed and protein efficiency ratio, serum (BA, LZY, PA and NBT), mucus (LZY and PA), superoxide dismutase, malondialdehyde and mucus secretion were significantly affected by either LP or VC (P < 0.05). Furthermore, only LP was a significant factor on survival, plasma total cholesterol, mucus BA and alternative complement pathway (P < 0.05). However, VC supplementation affected on HCT and LT50. Interestingly, fish fed with both LP at 1 g kg(-1) diet with VC at 0.5 or 1 g kg(-1) diet showed higher growth, humoral and mucosal immune responses, anti-oxidative status, mucus secretion and LT50 as well as decreased plasma, triglyceride and total cholesterol levels than the fish fed control diet (P < 0.05). These results demonstrated that dietary LP and VC had a significant interaction for red sea bream with the capability of improving growth performance and enhancing stress resistance by immunomodulation. PMID:27095173

  7. Dietary effects of adenosine monophosphate to enhance growth, digestibility, innate immune responses and stress resistance of juvenile red sea bream, Pagrus major.

    Science.gov (United States)

    Hossain, Md Sakhawat; Koshio, Shunsuke; Ishikawa, Manabu; Yokoyama, Saichiro; Sony, Nadia Mahjabin

    2016-09-01

    Our study explored the dietary effects of adenosine monophosphate (AMP) to enhance growth, digestibility, innate immune responses and stress resistance of juvenile red sea bream. A semi-purified basal diet supplemented with 0% (Control), 0.1% (AMP-0.1), 0.2% (AMP-0.2), 0.4% (AMP-0.4) and 0.8% (AMP-0.8) purified AMP to formulate five experimental diets. Each diet was randomly allocated to triplicate groups of fish (mean initial weight 3.4 g) for 56 days. The results indicated that dietary AMP supplements tended to improve growth performances. One of the best ones was found in diet group AMP-0.2, followed by diet groups AMP-0.1, AMP-0.4 and AMP-0.8. The Apparent digestibility coefficients (dry matter, protein and lipid) also improved by AMP supplementation and the significantly highest dry matter digestibility was observed in diet group AMP-0.2. Fish fed diet groups AMP-0.2 and AMP-0.4 had significantly higher peroxidase and bactericidal activities than fish fed the control diet. Nitro-blue-tetrazolium (NBT) activity was found to be significantly (P  0.05) by dietary supplementation. In contrast, catalase activity decreased with AMP supplementation. Moreover, the fish fed AMP supplemented diets had better improvement (P stress resistances. Interestingly, the fish fed diet groups AMP-0.2 and AMP-0.4 showed the least oxidative stress condition. Finally it is concluded that, dietary AMP supplementation enhanced the growth, digestibility, immune response and stress resistance of red sea bream. The regression analysis revealed that a dietary AMP supplementation between 0.2 and 0.4% supported weight gain and lysozyme activity as a marker of immune functions for red sea bream, which is also inline with the most of the growth and health performance parameters of fish under present experimental conditions. PMID:27514786

  8. Hepatic β-Oxidation and Regulation of Carnitine Palmitoyltransferase (CPT) I in Blunt Snout Bream Megalobrama amblycephala Fed a High Fat Diet

    OpenAIRE

    Lu, Kang-Le; Xu, Wei-Na; Wang, Li-na; Zhang, Ding-Dong; Zhang, Chun-Nuan; Liu, Wen-Bin

    2014-01-01

    High-fat diets may promote growth, partly through their protein-sparing effects. However, high-fat diets often lead to excessive fat deposition, which may have a negative impact on fish such as poor growth and suppressive immune. Therefore, this study investigated the effects of a fat-rich diet on the mechanisms of fat deposition in the liver. Three-hundred blunt snout bream (Megalobrama amblycephala) juveniles (initial mass 18.00±0.05 g) were fed with one of two diets (5% or 15% fat) for 8 w...

  9. MARICULTURE ON CROATIAN ISLANDS

    Directory of Open Access Journals (Sweden)

    Gordana Šarušić

    2000-09-01

    Full Text Available The first attempts of intensive mariculture in Croatia commenced at the very beginning of 1980’s. The mid-eighties brought an expansion of mariculture production, which has been continuously increasing. A few different marine organisms are intensively cultured - both fish and shellfish. Among them commercially most important and highly valued species are sea bass Dicentrarchus labrax and sea bream Sparus aurata. Mussel Mytilus galloprovincialis and oyster Ostrea edulis are the most important shellfish. Fish species such as dentex Dentex dentex, red sea bream Pagrus major and sheepshead bream Puntazzo puntazzo are reared too, but in a rather small quantities. Only recently the rearing, on-growing- of bluefin tuna Thunnus thynnus started in Croatia. The juveniles (70% are reared in a Croatian hatcheries, and 30% has to be imported mainly from Italy and France, due to a higher demand for this kind of culture among the small growers. Croatian part of Adriatic sea possesses a number of geomorfologicaly suitable sites and meteorological conditions which determined the choice - type - of intensive culture. All fish species are reared in a floating cages. The choice of cages i. e. semi off-shore or floating frames, size, rearing volume and design depend on the investors personal preference. The annual turnouf of a market size bass was about 600t and 300t bream in 1996., by 10 island farms which is 70% of total production in Croatia. Including other cultured fish species last year production was up to 1000t, and it™s being estimated to be about 1300t in the following year. The shellfish production on the islands is usually individual attempt of farmers, producing minor quantities mostly in polyculture. This production has bigger potential but it’s limited owing to the EU quality control regulations which do not allow the export, and by domestic market which has drastically decreased due to the collapse of tourism during the recent war. Almost 80

  10. CYTOTOXICITY AND GENOTOXICITY OF POLYETHYLENIMINE AND NICKEL CHLORIDE IN RED SEA BREAM (Pagrosomus major) FIN CELL LINE RSBF

    Institute of Scientific and Technical Information of China (English)

    郭华荣; 张士璀

    2002-01-01

    A continuous marine fish cell line RSBF (i.e. Red Sea Bream Fin) w as utilized to screen the cytotoxicity and genotoxicity of polyethylenimine (PEI) and nickel chloride (NiCl2) in this study on the deleterious effects of aquatic genotoxins on fish. At the 0.01 to 1 μg/ml concentration tested, PEI had acute toxicity to the treated RSBF cells (IC50 =1.12, 0.92, 0.88 and 0.64 μg/ml PEI for time 0 h, 24 h, 48 h and 72 h after treatment, respectively ) and markedly inhibited their proliferation in a dose-dependent manner. At the 0.001 to 5 μ mol/L concentration tested, NiCl2 posed no acute toxicity but significantly stimulated their growt h (107%-214% of control). Random amplified polymorphic DNA (RAPD) technique was used to detect the genotoxic effects of PEI and NiCl2 by comparing the RAPD banding patterns of t he control and treated cells. RAPD analysis indicated that at the concentrations tested, PEI w as more genotoxic than NiCl2 to RSBF cells; that there was a slight dose-dep endent response in the genotoxic effect of PEI but not NiCl2; and that RAPD technique might provide a sensitive, non -specific genotoxic endpoint. And the potent cytotoxicity and genotoxicity of PEI on fish cells sho wed that we should be cautious in utilizing it as gene vector in fish gene transfer and human gene therapy.

  11. Regulation of glucose and lipid metabolism by dietary carbohydrate levels and lipid sources in gilthead sea bream juveniles.

    Science.gov (United States)

    Castro, Carolina; Corraze, Geneviève; Firmino-Diógenes, Alexandre; Larroquet, Laurence; Panserat, Stéphane; Oliva-Teles, Aires

    2016-07-01

    The long-term effects on growth performance, body composition, plasma metabolites, liver and intestine glucose and lipid metabolism were assessed in gilthead sea bream juveniles fed diets without carbohydrates (CH-) or carbohydrate-enriched (20 % gelatinised starch, CH+) combined with two lipid sources (fish oil; or vegetable oil (VO)). No differences in growth performance among treatments were observed. Carbohydrate intake was associated with increased hepatic transcripts of glucokinase but not of 6-phosphofructokinase. Expression of phosphoenolpyruvate carboxykinase was down-regulated by carbohydrate intake, whereas, unexpectedly, glucose 6-phosphatase was up-regulated. Lipogenic enzyme activities (glucose-6-phosphate dehydrogenase, malic enzyme, fatty acid synthase) and ∆6 fatty acyl desaturase (FADS2) transcripts were increased in liver of fish fed CH+ diets, supporting an enhanced potential for lipogenesis and long-chain PUFA (LC-PUFA) biosynthesis. Despite the lower hepatic cholesterol content in CH+ groups, no influence on the expression of genes related to cholesterol efflux (ATP-binding cassette G5) and biosynthesis (lanosterol 14 α-demethylase, cytochrome P450 51 cytochrome P450 51 (CYP51A1); 7-dehydrocholesterol reductase) was recorded at the hepatic level. At the intestinal level, however, induction of CYP51A1 transcripts by carbohydrate intake was recorded. Dietary VO led to decreased plasma phospholipid and cholesterol concentrations but not on the transcripts of proteins involved in phospholipid biosynthesis (glycerol-3-phosphate acyltransferase) and cholesterol metabolism at intestinal and hepatic levels. Hepatic and muscular fatty acid profiles reflected that of diets, despite the up-regulation of FADS2 transcripts. Overall, this study demonstrated that dietary carbohydrates mainly affected carbohydrate metabolism, lipogenesis and LC-PUFA biosynthesis, whereas effects of dietary lipid source were mostly related with tissue fatty acid composition

  12. Evaluation of the fishery status for King Soldier Bream Argyrops spinifer in Pakistan using the software CEDA and ASPIC

    Science.gov (United States)

    Memon, Aamir Mahmood; Liu, Qun; Memon, Khadim Hussain; Baloch, Wazir Ali; Memon, Asfandyar; Baset, Abdul

    2015-07-01

    Catch and effort data were analyzed to estimate the maximum sustainable yield (MSY) of King Soldier Bream, Argyrops spinifer (Forsskål, 1775, Family: Sparidae), and to evaluate the present status of the fish stocks exploited in Pakistani waters. The catch and effort data for the 25-years period 1985-2009 were analyzed using two computer software packages, CEDA (catch and effort data analysis) and ASPIC (a surplus production model incorporating covariates). The maximum catch of 3 458 t was observed in 1988 and the minimum catch of 1 324 t in 2005, while the average annual catch of A. spinifer over the 25 years was 2 500 t. The surplus production models of Fox, Schaefer, and Pella Tomlinson under three error assumptions of normal, log-normal and gamma are in the CEDA package and the two surplus models of Fox and logistic are in the ASPIC package. In CEDA, the MSY was estimated by applying the initial proportion (IP) of 0.8, because the starting catch was approximately 80% of the maximum catch. Except for gamma, because gamma showed maximization failures, the estimated results of MSY using CEDA with the Fox surplus production model and two error assumptions, were 1 692.08 t ( R 2=0.572) and 1 694.09 t ( R 2=0.606), respectively, and from the Schaefer and the Pella Tomlinson models with two error assumptions were 2 390.95 t ( R 2=0.563), and 2 380.06 t ( R 2=0.605), respectively. The MSY estimated by the Fox model was conservatively compared to the Schaefer and Pella Tomlinson models. The MSY values from Schaefer and Pella Tomlinson models were the same. The computed values of MSY using the ASPIC computer software program with the two surplus production models of Fox and logistic were 1 498 t ( R 2=0.917), and 2 488 t ( R 2=0.897) respectively. The estimated values of MSY using CEDA were about 1 700-2 400 t and the values from ASPIC were 1 500-2 500 t. The estimates output by the CEDA and the ASPIC packages indicate that the stock is overfished, and needs some

  13. Identification of a novel transforming growth factor-β (TGF-β6 gene in fish: regulation in skeletal muscle by nutritional state

    Directory of Open Access Journals (Sweden)

    Jakowlew Sonia B

    2010-05-01

    Full Text Available Abstract Background The transforming growth factor-β (TGF-β family constitutes of dimeric proteins that regulate the growth, differentiation and metabolism of many cell types, including that of skeletal muscle in mammals. The potential role of TGF-βs in fish muscle growth is not known. Results Here we report the molecular characterization, developmental and tissue expression and regulation by nutritional state of a novel TGF-β gene from a marine fish, the gilthead sea bream Sparus aurata. S. aurata TGF-β6 is encoded by seven exons 361, 164, 133, 111, 181, 154, and 156 bp in length and is translated into a 420-amino acid peptide. The exons are separated by six introns: >643, 415, 93, 1250, 425 and >287 bp in length. Although the gene organization is most similar to mouse and chicken TGF-β2, the deduced amino acid sequence represents a novel TGF-β that is unique to fish that we have named TGF-β6. The molecule has conserved putative functional residues, including a cleavage motif (RXXR and nine cysteine residues that are characteristic of TGF-β. Semi-quantitative analysis of TGF-β6 expression revealed differential expression in various tissues of adult fish with high levels in skin and muscle, very low levels in liver, and moderate levels in other tissues including brain, eye and pituitary. TGF-β6 is expressed in larvae on day of hatching and increases as development progresses. A fasting period of five days of juvenile fish resulted in increased levels of TGF-β6 expression in white skeletal muscle compared to that in fed fish, which was slightly attenuated by one injection of growth hormone. Conclusion Our findings provide valuable insights about genomic information and nutritional regulation of TGF-β6 which will aid the further investigation of the S. aurata TGF-β6 gene in association with muscle growth. The finding of a novel TGF-β6 molecule, unique to fish, will contribute to the understanding of the evolution of the TGF

  14. Are retailers' preferences for seafood attributes predictive for consumers wants?

    DEFF Research Database (Denmark)

    Fernández-Polancoa, José; Mueller Loose, Simone; Luna, Ladislao

    2013-01-01

    Aquaculture production has grown considerably in the southern countries of Europe during the last two decades. This increase in supply has not been matched by an equivalent rise in consumer demand, resulting in price decay. For farmed seabream (Sparus aurata) this paper examines which attributes ...

  15. Oncomiracidial development, survival and swimming behaviour of the monogenean Sparicotyle chrysophrii (Van Beneden and Hesse, 1863)

    Czech Academy of Sciences Publication Activity Database

    Repulles-Albelda, A.; Holzer, Astrid S.; Raga, J. A.; Montero, F. E.

    338-341, MAR 29 (2012), s. 47-55. ISSN 0044-8486 Institutional research plan: CEZ:AV0Z60220518 Keywords : Sparicotyle chrysophrii * Sparus aurata * Polyopisthocotylea * Oncomiracidial development * Hatching * Survival time Subject RIV: GJ - Animal Vermins ; Diseases, Veterinary Medicine Impact factor: 2.009, year: 2012 http://www.sciencedirect.com/science/article/pii/S0044848612000646

  16. Host–parasite relationship of Ceratomyxa puntazzi n. sp. (Myxozoa: Myxosporea) and sharpsnout seabream Diplodus puntazzo (Walbaum, 1792) from the Mediterranean with first data on ceratomyxid host specificity in sparids

    Czech Academy of Sciences Publication Activity Database

    Alama-Bermejo, G.; Raga, J. A.; Holzer, Astrid S.

    2011-01-01

    Roč. 182, 2/4 (2011), s. 181-192. ISSN 0304-4017 Institutional research plan: CEZ:AV0Z60220518 Keywords : Myxozoa * Ceratomyxa puntazzi * Diplodus puntazzo * Sparus aurata and Diplodus annularis * Histopathology * SSU rDNA phylogeny * Transmission electron microscopy Subject RIV: GJ - Animal Vermins ; Diseases, Veterinary Medicine Impact factor: 2.579, year: 2011

  17. Effects of cadmium, naphthalene, and DDVP on gut carbohydrases activity in bream (Abramis brama L. ) and Mozambique tilapia (Oreochromis mossambicus Peters)

    Energy Technology Data Exchange (ETDEWEB)

    Golovanova, I.L.; Chuiko, G.M.; Pavlov, D.F. (Institute of the Biology of Inland Waters, Borok (Russian Federation))

    1994-03-01

    Previous research has shown that sublethal concentrations of cadmium, naphthalene and dichlorvos (DDVP) decreased growth rates in bream and Mozambique tilapia. One of the factors known to affect fish growth is the activity of gut digestive enzymes such as of lipases, proteases, carbohydrases. We assumed that toxicant-induced inhibition of the digestive enzyme activity and, consequently, the impaired digestion of food may contribute to the reduction of growth in fish exposed to toxicants. However, the influence of toxicants on digestive enzyme activities is poorly studied. The contribution of toxicant-induced changes of digestive enzymes activity to growth rate retardation in exposed fish remains unknown. The goal of this study was to examine the influence of an organophosphorus insecticide DDVP, a polyaromatic hydrocarbon naphthalene, and a metal cadmium on fish gut carbohydrase (CH) activity. 14 refs., 2 tabs.

  18. Comparative analysis and evolutionary significance of the HMG1 gene in crucian carp,blunt snout bream,and their polyploid progeny

    Institute of Scientific and Technical Information of China (English)

    Dong Liu; Zhen Liu; Shaojun Liu; Liangguo Liu; Cuiping You; Lin Chen; Huan Zhong; Yun Liu

    2009-01-01

    The full-length mRNA of the high mobility group protein 1 coding gene (HMG1) was obtained by RACE-PCR from red crucian carp (Carassius auratus red var.),blunt snout bream (Megalobrama amblycephala),and their triploid and tetraploid progeny.The sequence contained an open reading frame of 579 nucleotides coding for 193 amino acids.The nucleotide identity of HMG1 was higher between the tetraploid hybrid and the maternal red crucian carp (99%) than between the tetraploid hybrid and the paternal blunt snout bream (97%).The nucleotide identity between the triploid hybrids and each parent (95%) was lower than that between the parents (98%).The protein identity between the tetraploid hybrid and each parent (100%) was higher than that between the triploid hybrid and each parent (97%).Our results suggest that interspecific hybridization generates a shock to the HMG1 gene in triploid hybrids,causing divergence of nucleotides.The HMG1 protein of the tetraploid hybrids was consistent with that of its parents,which reduced the barrier of cross incompatibility between alleles,providing the basis for the bisexual fertile tetraploid hybrids forming a new polyploid species in nature.The secondary and tertiary structures of the HMG1 protein contain eight helices,three switches,two DNA-binding domains in the N-terminus,and a long acidic tail in the C-terminus.Together,these data suggest that the HMG1 protein plays a role of protein-DNA interactions,facilitating various DNA-dependent activities in the nucleus.We also investigated the phylogeny of fish,amphibian,reptilian,bird,and mammalian HMG1 proteins.Our results suggest that HMG1 is an ancestral protein that has been highly conserved.These data provide clues as to how interspecific hybridization may form polyploid hybrids.

  19. Effects of various LED light spectra on antioxidant and immune response in juvenile rock bream, Oplegnathus fasciatus exposed to bisphenol A.

    Science.gov (United States)

    Choi, Ji Yong; Kim, Tae Hwan; Choi, Young Jae; Kim, Na Na; Oh, Sung-Yong; Choi, Cheol Young

    2016-07-01

    Bisphenol A (BPA) is a monomer used in plastics and plasticizers. As an environmental toxin included in industrial wastewater, it contaminates the aquatic environment and is known to cause endocrine disruption in fish. Particular wavelengths of light-emitting diodes (LEDs) are known to affect the endocrine regulation of fish. The present study aimed to investigate the effects of green and red LED light on the antioxidant and immune systems in juvenile rock bream (Oplegnathus fasciatus) exposed to BPA. We used green and red LED exposure at two intensities (0.3 and 0.5W/m(2)) for 1, 3, and 5 days. We measured liver mRNA expression and plasma levels of antioxidant enzyme superoxide dismutase (SOD) and caspase-3. Furthermore, we measured plasma levels of hydrogen peroxide (H2O2), lipid peroxidation (LPO), melatonin, and immunoglobulin M (IgM). DNA damage and apoptotic activity were measured using comet and terminal transferase dUTP nick end labeling (TUNEL) assays, respectively. We found that SOD, H2O2, and LPO increased significantly, whereas melatonin and IgM decreased significantly, suggesting that BPA induces oxidative stress and reduces immune function. Likewise, both DNA damage and apoptotic activity increased following BPA exposure. However, we found that exposure to green LED light effectively reduced the detrimental effects induced by BPA, including decreasing DNA damage, apoptotic activity, SOD mRNA expression, and plasma levels of SOD, H2O2, and LPO. Likewise, the plasma levels of melatonin and IgM increased. Thus, our results indicate that green light conditions effectively reduces oxidative stress and promotes the immune function in juvenile rock bream. PMID:27299659

  20. Combined effects of dietary fructooligosaccharide and Bacillus licheniformis on innate immunity, antioxidant capability and disease resistance of triangular bream (Megalobrama terminalis).

    Science.gov (United States)

    Zhang, Chun-Nuan; Li, Xiang-Fei; Xu, Wei-Na; Jiang, Guang-Zhen; Lu, Kang-Le; Wang, Li-Na; Liu, Wen-Bin

    2013-11-01

    This study was conducted to investigate the effects of fructooligosaccharide (FOS) and Bacillus licheniformis (B. licheniformis) and their interaction on innate immunity, antioxidant capability and disease resistance of triangular bream Megalobrama terminalis (average initial weight 30.5 ± 0.5 g). Nine experimental diets were formulated to contain three FOS levels (0, 0.3% and 0.6%) and three B. licheniformis levels (0, 1 × 10(7), 5 × 10(7) CFU g(-1)) according to a 3 × 3 factorial design. At the end of the 8-week feeding trial, fish were challenged by Aeromonas hydrophila (A. hydrophila) and survival rate was recorded for the next 7 days. The results showed that leucocyte counts, alternative complement activity as well as total serum protein and globulin contents all increased significantly (P 0.05) was observed in these parameters in terms of dietary FOS levels. Both plasma alkaline phosphatase and phenoloxidase activities were significantly (P FOS levels with the highest values observed in fish fed 0.6 and 0.3% FOS, respectively. Both immunoglobulin M content and liver superoxide dismutase (SOD) activity were significantly affected (P > 0.05) by both FOS and B. licheniformis. Liver catalase, glutathione peroxidase as well as plasma SOD activities of fish fed 1 × 10(7) CFU g(-1)B. licheniformis were all significantly (P 0.05) by either FOS levels or B. licheniformis contents, whereas a significant (P FOS and 1 × 10(7) CFU g(-1)B. licheniformis. The results of this study indicated that dietary FOS and B. licheniformis could significantly enhance the innate immunity and antioxidant capability of triangular bream, as well as improve its disease resistance. The best combination of these two prebiotics and/or probiotics was 0.3% FOS and 1 × 10(7) CFU g(-1)B. licheniformis. PMID:23932988

  1. Chemometric analysis of fatty acids profile of bream (Abramis brama), ruffe (Gymnocephalus cernua) and perch (Perca fluviatilis) meat from Lake Gopło and Włocławski Dam Reservoir

    OpenAIRE

    Bogumila Kupcewicz; Magdalena STANEK; Bogdan JANICKI

    2012-01-01

    The 18 fatty acid profiles have been determined in 63 samples of muscles from three freshwater fish species: bream, ruffe and perch by gas chromatography method. The fish were collected in natural condition from two reservoirs located in central Poland: Lake Gopło and Włocławski Reservoir. A chemometric study with the use of hierarchical cluster analysis (HCA), principal component (PCA) and stepwise linear discrimination analysis (LDA) was applied to characterize, classify and differentiate c...

  2. Effects of protein hydrolysates supplementation in low fish meal diets on growth performance, innate immunity and disease resistance of red sea bream Pagrus major.

    Science.gov (United States)

    Khosravi, Sanaz; Rahimnejad, Samad; Herault, Mikaël; Fournier, Vincent; Lee, Cho-Rong; Dio Bui, Hien Thi; Jeong, Jun-Bum; Lee, Kyeong-Jun

    2015-08-01

    This study was conducted to evaluate the supplemental effects of three different types of protein hydrolysates in a low fish meal (FM) diet on growth performance, feed utilization, intestinal morphology, innate immunity and disease resistance of juvenile red sea bream. A FM-based diet was used as a high fish meal diet (HFM) and a low fish meal (LFM) diet was prepared by replacing 50% of FM by soy protein concentrate. Three other diets were prepared by supplementing shrimp, tilapia or krill hydrolysate to the LFM diet (designated as SH, TH and KH, respectively). Triplicate groups of fish (4.9 ± 0.1 g) were fed one of the test diets to apparent satiation twice daily for 13 weeks and then challenged by Edwardsiella tarda. At the end of the feeding trial, significantly (P HFM and hydrolysate treated groups compared to those fed the LFM diet. Significant improvements in feed conversion and protein efficiency ratios were obtained in fish fed the hydrolysates compared to those fed the LFM diet. Significant enhancement in digestibility of protein was found in fish fed SH and KH diets and dry matter digestibility was increased in the group fed SH diet in comparison to LFM group. Fish fed the LFM diet showed significantly higher glucose level than all the other treatments. Whole-body and dorsal muscle compositions were not significantly influenced by dietary treatments. Histological analysis revealed significant reductions in goblet cell numbers and enterocyte length in the proximal intestine of fish fed the LFM diet. Superoxide dismutase activity and total immunoglobulin level were significantly increased in fish fed the diets containing protein hydrolysates compared to the LFM group. Also, significantly higher lysozyme and antiprotease activities were found in fish fed the hydrolysates and HFM diets compared to those offered LFM diet. Fish fed the LFM diet exhibited the lowest disease resistance against E. tarda and dietary inclusion of the hydrolysates resulted in

  3. ANTILISTERIAL ACTYVITY OF LACTIC ACID BACTERIA ISOLATED FROM GILTHEAD BREAMS AND SEA BASSES FILLETS PACKAGED MAP AGAINST PRIMITIVE STRAINS OF LISTERIA MONOCYTOGENES

    Directory of Open Access Journals (Sweden)

    M. Barile

    2011-04-01

    Full Text Available Listeria monocytogenes is the causative agent of listeriosis typically caused by ready-to-eat processed food that have a refrigerated shelf-life, but lightly preserved fish products also belong to a high-risk category. Aim of the work was to evaluate antimicrobial activity linked bacteriocin-producing of LAB isolated from gilthead breams and sea basses fillets packaged in modified atmospheres. Fifty-five LAB strains were screened against 21 strains of Listeria monocytogenes, 1 Listeria innocua held in the culture collection of Department of Zootechnical Sciences and Food Ispection (SIA and submitted to antagonistic activity using the spot on lawn and the agar well diffusion assay. Lactococcus lactis sub. lactis Sa31 was able to produce bacteriocin in agar and different broth medium. The bacteriocin man31 showed sensitivity to trypsin, pronase E and papain, inactivation at temperatures ≥ 100°C, bactericidal mode of action and antilisterial act, rapidly. The bacteriocin man31 caused a reduction of L. monocytogenes ½ c growth about log10 > 3 UFC/ml, when was applied on indicator strain at 20,480 AU/ml concentration, in vitro.

  4. Biometric parameters of the bream (Abramis brama) as indicators for long-term changes in fish health and environmental quality--data from the German ESB.

    Science.gov (United States)

    Teubner, Diana; Paulus, Martin; Veith, Michael; Klein, Roland

    2015-02-01

    Piscifaunal health depends upon the state and quality of the aquatic environment. Variations in physical condition of fish may therefore be attributed to changes in environmental quality. Based on time series of up to 20 years of biometric data of bream from multiple sampling sites of the German environmental specimen bank (ESB), this study assessed whether changes in biometric parameters are able to indicate long-term alterations in fish health and environmental quality. Evaluated biometric parameters of fish health comprised length and weight of individuals of a defined age class, the condition factor, lipid content and hepatosomatic index (HSI). Although there are negative trends of the HSI, the overall development of health parameters can be interpreted as positive. This seems to suggest that health parameters conclusively mirror the long-term improvement of water quality in the selected rivers. However, the applicability of the condition factor as well as lipid content as indicators for fish health remained subject to restrictions. Altogether, the results from the ESB confirmed the high value of biometric parameters for monitoring of long-term changes in state and quality of aquatic ecosystems. PMID:24824506

  5. Amnesic shellfish poisoning toxin stimulates the transcription of CYP1A possibly through AHR and ARNT in the liver of red sea bream Pagrus major

    International Nuclear Information System (INIS)

    To investigate the role of detoxification-related liver genes in amnesic shellfish poisoning toxin metabolism, red sea bream Pagrus major were exposed to domoic acid (DA, 2 μg g-1 wet weight) for 24 h. Hepatic mRNA expression levels of AHR, ARNT, CYP1 and GSTs were determined by semi-quantitative RT-PCR. The cytosolic factors aryl hydrocarbon receptor (AHR) and aryl hydrocarbon receptor nuclear translocator (ARNT) mRNA levels of DA exposure group were substantially enhanced by 113.3% and 90.9%, respectively. Consistent with this result, the phase I xenobiotic metabolizing enzyme (XME) cytochrome P-450 1A (CYP1A) was significantly induced. In contrast, the transcriptions of three major phase II XME glutathione S-transferases as well as heat shock protein 70 were not significantly affected by DA exposure. These results suggest a possible role of CYP1A after DA exposure in the toxin metabolism of marine fish, possibly through the AHR/ARNT signaling pathway.

  6. Parental contribution and growth hormone gene polymorphism associated with growth phenotypes of red sea bream Pagrus major in mass production: A case study

    Directory of Open Access Journals (Sweden)

    Eitaro Sawayama

    2015-11-01

    Full Text Available Red sea bream is one of the most important aquaculture fish species in Japan. To improve the productivity of this fish during seed production, improved growth traits and reduced size variation are needed. In this study, we assessed parental contribution of fast- and slow-growing individuals observed in two different rearing phases in a mass production lot: (1 50 dph reared in a tank and (2 200 dph reared in a net cage. We also assessed GH gene (pmaGH polymorphisms based on a previously developed minisatellite DNA marker. Specific broodstock individuals were significantly associated with fast- or slow-growing individuals at 50 dph and 200 dph. Significant differences in pmaGH minisatellite allele frequencies were observed between fast- and slow-growing groups at 50 dph in the frequency of two alleles (pmaGH-740 and pmaGH-900, respectively. Combining the results of DNA parentage analysis and pmaGH minisatellite allele analysis, one dam and two sires, possessing pmaGH-740, were significantly associated with the slow-growing groups. These results suggest that the minisatellite marker of pmaGH could be a useful tool for growth selection of this fish species.

  7. Bacterial vaccines for fish--an update of the current situation worldwide.

    Science.gov (United States)

    Håstein, T; Gudding, R; Evensen, O

    2005-01-01

    During the last few years, the use of vaccines for disease prevention in aquaculture has expanded both with regard to the number of fish species and number of microbial diseases. According to the responses to a questionnaire received from 41 countries, vaccination is used in the commercial aquaculture of species like Atlantic salmon (Salmo salar), rainbow trout (Oncorhynchus mykiss), sea bass (Dicentrarchus labrax), sea bream (Sparus aurata), barramundi (Lates calcarifer), tilapia (Tilapia spp), turbot (Scophthalmus maximus L.), yellowtail (Seriola quinqueradiata), purplish and gold-striped amberjack (Seriola dumereli), striped jack (Pseudocaranx dentex) and channel catfish (Ictalurus punctatus). The range of bacterial infections for which vaccines are commercially available now comprises classical vibriosis (Listonella anguillarum, Vibrio ordalii), furunculosis (Aeromonas salmonicida subsp. salmonicida), cold-water vibriosis (Vibrio salmonicida), yersiniosis (Yersinia ruckeri), pasteurellosis (Photobacterium damselae supsp. piscicida), edwardsiellosis (Edwardsiella ictaluri), winter ulcer (Moritella viscosa), and streptococcosis/lactococcosis (Streptococcus iniae, Lactococcus garviae). Furthermore, experimental vaccines are used against diseases such as infection with Vibrio harveyi and Photobacterium damsela subsp. damsela in barramundi, piscirickettsiosis and bacterial kidney disease in salmonids, as well as infection with Flexibacter maritimus (now: Tenacibaculum maritimum) in turbot. There was good agreement between the information received from different sources in the same country. Most vaccines are licensed products, but some non-licensed vaccines are also used in commercial fish farms. Most bacterial vaccines are inactivated products and recombinant vaccine technology has so far been used to a very limited extent. Salmonid fish are usually immunised with multivalent vaccines by intraperitoneal injection. In marine fish species vaccination is generally

  8. PHYTOPLANKTON COMPOSITION IN FISH FARMS ALONG THE EASTERN ADRIATIC COAST

    Directory of Open Access Journals (Sweden)

    Marija Tomec

    2006-10-01

    Full Text Available Investigations of net phytoplankton composition were performed at three fish farms situated at the northern, middle and southern part of the eastern Adriatic Sea coast, respectively. In the northern part investigations were conducted in the Limski kanal, in the middle part at the Ugljan island and in the southern part in the place Drače on the Pelješac peninsula (Figure 1. At all three localities fish culture included mostly two species: gilthead sea bream (Sparus aurata and sea bass (Dicentrarchus labrax. Beside some physico–chemical parameters (sea water temperature, salinity special attention was placed on the examination of qualitative net phytoplankton composition, which was conducted in the period of May and November 2004 and May and October 2005. Samples were collected at the depths of 0. 5 and 4 meters. According to the physico–chemical parameters, sea water temperature was influenced by the temperature of the environment. Qualitative net phytoplankton composition consisted of 153 microphytic species belonging to the systematic compartments of Cyanobacteria, Chrysophyta and Dinophyta (Table 1. The most numerous algal group were diatoms or Bacillarophyceae (84 species or 55% with relative frequencies of species from 1 to 7. Taxonomic composition of diatoms showed the community Chaetoceros–Rhizosolenia (Proboscia as the dominant one. The second numerically most dominant compartment were Dinophyta (62 species or 401% with dominant the species of the genera Ceratium and Protoperidinium. Relative frequencies of species was ranging from 1 to 7 (mass presence of specimens in the water column. From Cyanobacteria (4 species or 3%, only filamentous algae were determined, with individual presence in net phytoplankton composition. Qualitative net phytoplankton composition suggests the similarity of species composition in the water column at all investigated fish farms. From the obtained characteristics of net phytoplankton composition

  9. 10种人工鱼礁模型对黑鲷幼鱼的诱集效果%Attraction effect of various artificial reef models on Sparus macrocephalus

    Institute of Scientific and Technical Information of China (English)

    周艳波; 蔡文贵; 陈海刚; 陈丕茂; 吕国敏; 贾晓平

    2011-01-01

    Artificial reef was used in marine ranch to improve the marine ecological environment and enhance the fishery resources. Attractive effects of artificial reef may be attributed to creating habitat, shelter and spawning ground for the various kinds of fish, therefore positively affect the breeding and growth of the fish. In recent years, artificial reef construction programs have been rapidly developed in China. However, the knowledge of the effect of artificial reef on marine ecological environment is limited. The present study aimed to determine the influence of materials, structure and combination on the attractive effects of the artificial reefs and therefore provide useful data and information for the development of artificial reef construction. In the present study, the effects of ten kinds of artificial reef models with different shapes, which have been deployed in Yangmeikeng reef area, on the behavioral responses of the fish Sparus macrocephalus and the fish attraction effects of the artificial reef models were analyzed and compared with those without the artificial reef model in the trough. The result showed that in the condition without the artificial reef model in the trough, the fish liked to stay in radius of the center trough area within 40 - 70 cm. while the fish are more dispersed in the trough. After the artificial model was put into the troughs, the fish liked to stay in radius of the center trough area within 20 - 50 cm while No. 1 reef-No. 8 reef were put into the trough, the fish liked to stay in radius of the center trough area within 10 -40 cm while No. 9 reef and No. 10 reef were put into the trough. The average occurring rate of the fish inside and near the reef areas all increased, while comparing fish occurring rates in artificial reef areas with or without artificial reef. The analysis results showed that all of the ten artificial reef models have obvious attraction effects on S. macrocephalus,especially for model NO. 10. Its attraction

  10. ECTOPARASITOSES OF CULTURED MARINE FISH

    OpenAIRE

    Dario Čičin-Karlov; Zlatica Teskeredžić

    2005-01-01

    Total of 70 individua collected on the four different fish farms were examined — 40 sea bass, 10 gilthead sea bream, 10 sharp–snouted sparus and 10 dentex. The investigations were focused on the presence of the ectoparasit Diplectanum sp. which belongs to platyhelmintes, monogena, diplectanidae, and the results of the examination are presented in this paper.

  11. Chemometric analysis of fatty acids profile of bream (Abramis brama, ruffe (Gymnocephalus cernua and perch (Perca fluviatilis meat from Lake Gopło and Włocławski Dam Reservoir

    Directory of Open Access Journals (Sweden)

    Bogumila Kupcewicz

    2012-01-01

    Full Text Available The 18 fatty acid profiles have been determined in 63 samples of muscles from three freshwater fish species: bream, ruffe and perch by gas chromatography method. The fish were collected in natural condition from two reservoirs located in central Poland: Lake Gopło and Włocławski Reservoir. A chemometric study with the use of hierarchical cluster analysis (HCA, principal component (PCA and stepwise linear discrimination analysis (LDA was applied to characterize, classify and differentiate collected samples. The chemometric techniques by using fatty acids content as descriptors allow clearly distinguish 6 groups according to fish species and their geographical origin.

  12. PHYTOPLANKTON COMPOSITION AT THE FISH AND SHELLFISH FARM IN THE KALDONTA BAY (CRES ISLAND

    Directory of Open Access Journals (Sweden)

    Marija Tomec

    2004-12-01

    Full Text Available The Kaldonta Bay is situated at the south–western coast of the Cres island in the Lošinj channel, rather protected from larger influence of general sea water current. In the Bay there are installed 44 floating cages of 5 by 10 m dimensions. The cages are used for the culture of about 70 tons of sea water fish: gilthead sea bream (Sparus aurata, sea bass (Dicentrarchus labrax, sharp–snouted sparus (Diplodus puntazzo and dentex (Dentex dentex. Besides some physico–chemical parameters (sea water temperature, transparence and salinity, special attention has been paid to the qualitative composition of net phytoplankton. Investigations were performed in the period of May, September and December 2003 and February 2004 at five locations in the Kaldonta Bay (Figure 1 at the depths of 0.5 m, 5 m, 10 m and 1 m from the bottom. According to the physico–chemical parameters, sea water temperature was influenced by the temperature of the environment, and the transparence suggested to the oligotrophic situation in the investigated aquatorium. Qualitative composition of net phytoplankton comprised 161 microphytic species belonging to the systematic compartments of Cyanobacteria, Chrysophyta and Dinophyta (Table 1. The most numerous algal group were diatoms or Bacillarophyceae (98 species or 61%, with relative frequencies of species from 1 to 7. Taxonomic composition of diatoms showed Chaetoceros–Rhizosolenia (Proboscia to be the dominant community. Diatom species was the most abundant in late autumn period (beginning of December. The second most important comparatment were Dinophyta (55 species or 34.1%, with the dominant genera Ceratium and Protoperidinium. During the investigation, the representatives of Dinophyta did not show large variety of species in the water column. Relative frequency of the species was 1, rarely 2 and 3. Dinophyts were the most abundant in September. From Cyanobacteria (5 species or 3.1% only filamentous algae were determined

  13. Hepatic β-oxidation and regulation of carnitine palmitoyltransferase (CPT) I in blunt snout bream Megalobrama amblycephala fed a high fat diet.

    Science.gov (United States)

    Lu, Kang-Le; Xu, Wei-Na; Wang, Li-Na; Zhang, Ding-Dong; Zhang, Chun-Nuan; Liu, Wen-Bin

    2014-01-01

    High-fat diets may promote growth, partly through their protein-sparing effects. However, high-fat diets often lead to excessive fat deposition, which may have a negative impact on fish such as poor growth and suppressive immune. Therefore, this study investigated the effects of a fat-rich diet on the mechanisms of fat deposition in the liver. Three-hundred blunt snout bream (Megalobrama amblycephala) juveniles (initial mass 18.00 ± 0.05 g) were fed with one of two diets (5% or 15% fat) for 8 weeks. β-Oxidation capacity and regulation of rate-limiting enzymes were assessed. Large fat droplets were present in hepatocytes of fish fed the high-fat diet. This observation is thought to be largely owing to the reduced capacity for mitochondrial and peroxisomal β-oxidation in the livers of fish fed the high-fat diet, as well as the decreased activities of carnitine palmitoyltransferase (CPT) I and acyl-CoA oxidase (ACO), which are enzymes involved in fatty-acid metabolism. Study of CPT I kinetics showed that CPT I had a low affinity for its substrates and a low catalytic efficiency in fish fed the high-fat diet. Expression of both CPT I and ACO was significantly down-regulated in fish fed the high-fat diet. Moreover, the fatty-acid composition of the mitochondrial membrane varied between the two groups. In conclusion, the attenuated β-oxidation capacity observed in fish fed a high-fat diet is proposed to be owing to decreased activity and/or catalytic efficiency of the rate-limiting enzymes CPT I and ACO, via both genetic and non-genetic mechanisms. PMID:24676148

  14. Effects of berberine on the growth and immune performance in response to ammonia stress and high-fat dietary in blunt snout bream Megalobrama amblycephala.

    Science.gov (United States)

    Chen, Qing-Qing; Liu, Wen-Bin; Zhou, Man; Dai, Yong-Jun; Xu, Chao; Tian, Hong-Yan; Xu, Wei-Na

    2016-08-01

    This study aimed to figure out the effects of berberine on growth performance, immunity, oxidative stress and hepatocyte apoptosis of blunt snout bream (Megalobrama amblycephala) fed with high-fat diet. 320 fish (80.00 ± 0.90 g) were divided randomly into four trial groups (each with four replicates) and fed with 4 diets (normal diet, normal diet with 50 mg/kg berberine, high-fat diet, high-fat diet with 50 mg/kg berberine), respectively. At the end of the feeding trial, ammonia stress test was carried out for 5 days. The result showed the growth performance, immune parameters including plasm acid phosphatase (ACP) activities, lysozyme (LYZ) activities and alternative complement C3 and C4 contents were suppressed in fish fed with high-fat diets but improved in berberine diets compared with control (normal diet). Hepatopancreas oxidative status, the malondialdehyde (MDA), protein carbonyl (PC) and lipid peroxide (LPO) were increased significantly (P < 0.05) when fish were fed with high-fat diets. Berberine could slow the progression of the oxidative stress induced by high-fat through increasing superoxide dismutase (SOD) activities and total sulfydryl (T-SH) levels of fish. And the hepatocyte apoptosis in the high-fat group could also be alleviated by berberine. After the ammonia stress test, the accumulative mortality was extremely (P < 0.05) low in fish fed high-fat diet with berberine compared to other groups. It was concluded berberine as a functional feed additive significantly inhibited the progression of oxidative stress, reduced the apoptosis and enhanced the immunity of fish fed with high-fat diet. PMID:27235371

  15. Effects of dietary supplementation of Lactobacillus rhamnosus or/and Lactococcus lactis on the growth, gut microbiota and immune responses of red sea bream, Pagrus major.

    Science.gov (United States)

    Dawood, Mahmoud A O; Koshio, Shunsuke; Ishikawa, Manabu; Yokoyama, Saichiro; El Basuini, Mohammed F; Hossain, Md Sakhawat; Nhu, Truong H; Dossou, Serge; Moss, Amina S

    2016-02-01

    Pagrus major fingerlings (3·29 ± 0·02 g) were fed with basal diet (control) supplemented with Lactobacillus rhamnosus (LR), Lactococcus lactis (LL), and L. rhamnosus + L. lactis (LR + LL) at 10(6) cell g(-1) feed for 56 days. Feeding a mixture of LR and LL significantly increased feed utilization (FER and PER), intestine lactic acid bacteria (LAB) count, plasma total protein, alternative complement pathway (ACP), peroxidase, and mucus secretion compared with the other groups (P < 0.05). Serum lysozyme activity (LZY) significantly increased in LR + LL when compared with the control group. Additionally, fish fed the LR + LL diet showed a higher growth performance (Fn wt, WG, and SGR) and protein digestibility than the groups fed an individual LR or the control diet. Superoxide dismutase (SOD) significantly increased in LR and LR + LL groups when compared with the other groups. Moreover, the fish fed LR or LL had better improvement (P < 0.05) in growth, feed utilization, body protein and lipid contents, digestibility coefficients (dry matter, protein, and lipid), protease activity, total intestine and LAB counts, hematocrit, total plasma protein, biological antioxidant potential, ACP, serum and mucus LZY and bactericidal activities, peroxidase, SOD, and mucus secretion than the control group. Interestingly, fish fed diets with LR + LL showed significantly lower total cholesterol and triglycerides when compared with the other groups (P < 0.05). These data strongly suggest that a mixture of LR and LL probiotics may serve as a healthy immunostimulating feed additive in red sea bream aquaculture. PMID:26766177

  16. The Taxonomic Status of Japanese Threadfin BreamNemipterus japonicus (Bloch, 1791) (Perciformes:Nemipteridae) with a Redescription of this Species from the South China Sea Based on Morphology and DNA Barcodes

    Institute of Scientific and Technical Information of China (English)

    NING Ping; SHA Zhongli; Paul DN HEBERT; Barry RUSSELL

    2015-01-01

    Because of its importance as a food source,Nemipterus japonicus(Bloch, 1791) (Nemipteridae) or Japanese threadfin bream is the best studied of these taxa, and numerous investigations have examined its fisheries, its biology and biochemistry. De-spite such intensive work, the taxonomic status ofN. japonicushas never been seriously questioned and itis regarded as a common species, widely distributed throughout the Indo-Western Pacific Ocean. In fact, Bloch’s description of the type specimen ofN. ja-ponicus has ambiguous collection data and lacks a designation for the type locality, though it is probably Java. In this paper, DNA barcode results based on COI gene support the existence of two geographically separated lineages of the Japanese threadfin bream, both being an Indian Ocean and western Pacific lineage, with 2.7% sequence divergence, and the results indicate a possible existing of some cryptic species. The two lineages also possess a diagnostic difference in their belly color, with specimens in the South China Sea having a silver belly, while those from the Indian Ocean isolate specimen have a yellow coloration. Based upon new collections from the South China Sea, this species from the western Pacific is morphologically redescribed and its details of DNA barcode diver-sity are shown for the future investigations.

  17. The taxonomic status of Japanese threadfin bream Nemipterus japonicus (Bloch, 1791) (Perciformes: Nemipteridae) with a redescription of this species from the south china sea based on morphology and DNA barcodes

    Science.gov (United States)

    Ning, Ping; Sha, Zhongli; Hebert, Paul D. N.; Russell, Barry

    2015-02-01

    Because of its importance as a food source, Nemipterus japonicus (Bloch, 1791) (Nemipteridae) or Japanese threadfin bream is the best studied of these taxa, and numerous investigations have examined its fisheries, its biology and biochemistry. Despite such intensive work, the taxonomic status of N. japonicus has never been seriously questioned and it is regarded as a common species, widely distributed throughout the Indo-Western Pacific Ocean. In fact, Bloch's description of the type specimen of N. japonicus has ambiguous collection data and lacks a designation for the type locality, though it is probably Java. In this paper, DNA barcode results based on COI gene support the existence of two geographically separated lineages of the Japanese threadfin bream, both being an Indian Ocean and western Pacific lineage, with 2.7% sequence divergence, and the results indicate a possible existing of some cryptic species. The two lineages also possess a diagnostic difference in their belly color, with specimens in the South China Sea having a silver belly, while those from the Indian Ocean isolate specimen have a yellow coloration. Based upon new collections from the South China Sea, this species from the western Pacific is morphologically redescribed and its details of DNA barcode diversity are shown for the future investigations.

  18. Structural and functional divergence of two fish aquaporin-1 water channels following teleost-specific gene duplication

    Directory of Open Access Journals (Sweden)

    Raldúa Demetrio

    2008-09-01

    Full Text Available Abstract Background Teleost radiation in the oceans required specific physiological adaptations in eggs and early embryos to survive in the hyper-osmotic seawater. Investigating the evolution of aquaporins (AQPs in these vertebrates should help to elucidate how mechanisms for water homeostasis evolved. The marine teleost gilthead sea bream (Sparus aurata has a mammalian aquaporin-1 (AQP1-related channel, termed AQP1o, with a specialized physiological role in mediating egg hydration. However, teleosts have an additional AQP isoform structurally more similar to AQP1, though its relationship with AQP1o is unclear. Results By using phylogenetic and genomic analyses we show here that teleosts, unlike tetrapods, have two closely linked AQP1 paralogous genes, termed aqp1a and aqp1b (formerly AQP1o. In marine teleosts that produce hydrated eggs, aqp1b is highly expressed in the ovary, whereas in freshwater species that produce non-hydrated eggs, aqp1b has a completely different expression pattern or is not found in the genome. Both Aqp1a and Aqp1b are functional water-selective channels when expressed in Xenopus laevis oocytes. However, expression of chimeric and mutated proteins in oocytes revealed that the sea bream Aqp1b C-terminus, unlike that of Aqp1a, contains specific residues involved in the control of Aqp1b intracellular trafficking through phosphorylation-independent and -dependent mechanisms. Conclusion We propose that 1 Aqp1a and Aqp1b are encoded by distinct genes that probably originated specifically in the teleost lineage by duplication of a common ancestor soon after divergence from tetrapods, 2 Aqp1b possibly represents a neofunctionalized AQP adapted to oocytes of marine and catadromous teleosts, thereby contributing to a water reservoir in eggs and early embryos that increases their survival in the ocean, and 3 Aqp1b independently acquired regulatory domains in the cytoplasmatic C-terminal tail for the specific control of Aqp1b

  19. Effects of dietary pantothenic acid on growth, intestinal function, anti-oxidative status and fatty acids synthesis of juvenile blunt snout bream Megalobrama amblycephala.

    Directory of Open Access Journals (Sweden)

    Yu Qian

    dietary PA requirements of juvenile blunt snout bream were estimated to be 24.08 mg kg(-1.

  20. Morphological differences between wild and farmed Mediterranean fish

    OpenAIRE

    Arechavala-Lopez, Pablo; Sanchez-Jerez, Pablo; Bayle-Sempere, Just T.; Sfakianakis, Dimitris G.; Somarakis, Stylianos

    2012-01-01

    Gilthead seabream (Sparus aurata L.) and European seabass (Dicentrarchus labrax L.) are important commercial marine fish species both for aquaculture and fisheries in the Mediterranean. It is known that farmed individuals escape from farm facilities, but the extent of escape events is not easy to report and estimate because of the difficulty to distinguish between wild and farmed individuals. In this study, significant differences provided through morphometry evidence that the cranial and bod...

  1. New Tetrachromic VOF Stain (Type III-G.S) for Normal and Pathological Fish Tissues

    OpenAIRE

    Sarasquete, C.; Gutiérrez, M.

    2005-01-01

    A new VOF Type III-G.S stain was applied to histological sections of different organs and tissues of healthy and pathological larvae, juvenile and adult fish species (Solea senegalensis; Sparus aurata; Diplodus sargo; Pagrus auriga; Argyrosomus regius and Halobatrachus didactylus). In comparison to the original Gutiérrez VOF stain, more acid dyes of contrasting colours and polychromatic/metachromatic properties were incorporated as essential constituents of the tetrachromic VOF stain. This fa...

  2. Bioaccumulation of Heavy Metals and Microelements in Silver Bream (Brama brama L.), Northern Pike (Esox lucius L.), Sterlet (Acipenser ruthenus L.), and Common Carp (Cyprinus carpio L.) From Tisza River, Serbia.

    Science.gov (United States)

    Štrbac, Snežana; Kašanin-Grubin, Milica; Jovančićević, Branimir; Simonović, Predrag

    2015-01-01

    The aim of this study was to determine the concentrations of Al, As, B, Cd, Co, Cr, Cu, Fe, Hg, Mn, Ni, Pb, Se, Sr, and Zn in liver, gills, gonads, and brain of four ecologically different fish species in Serbia: piscivorous northern pike, benthivorous sterlet and silver bream, and omnivorous common carp. Fish were caught at four sites along the stretch of the River Tisza in the Pannonian part of Serbia during October 2010. Results revealed that heavy metals and microelements with the highest values in fish samples were Fe, Al, and Zn. The highest concentration of heavy metals and microelements was recorded in omnivorous common carp, and organs that most intensively accumulated the greatest number of metals were liver and gills, whereas the locality did not exert a marked impact on level of bioaccumulation. PMID:26039743

  3. Arbeitsgemeinschaft fuer die Reinhaltung der Elbe - pollutant monitoring of the Elbe river using breams (Abramis brama L.). A classification system; Arbeitsgemeinschaft fuer die Reinhaltung der Elbe - Schadstoffueberwachung der Elbe mit der Fischart Brassen (Abramis brama L.). Ein Klassifizierungssystem

    Energy Technology Data Exchange (ETDEWEB)

    Krieg, H.J. [Hydrobiologische Untersuchungen und Gutachten (HUuG), Tangstedt (Germany); Gaumert, T.; Reincke, H. [Wasserguetestelle Elbe, Hamburg (Germany)

    2000-08-01

    In 1979-1980, breams caught between Schnackenburg and Glueckstadt were analyzed for heavy metals and chlorinated hydrocarbons. After the German unification, the analyses were extended to further sections of the river. The report presents the results. [German] In den Jahren 1979-1980 wurden Brassen zwischen Schnackenburg und Glueckstadt fuer eine gezielte Analyse auf verschiedene Schwermetalle und Chlorkohlenwasserstoffe gefangen. Vorrangiges Ziel der damaligen ARGE-ELBE-Untersuchung war die Eignung des Weissfisches als Bioindikator zur Darstellung der Schadstoffbelastung in der Elbe. Nach der Wiedervereinigung wurde ein weiteres Sondermessprogramm mit dem Brassen durchgefuehrt. Der Brassen wurde an 10 Fangplaetzen im bundesdeutschen Elbelaengsschnitt 1994 befischt: An zwei Stationen in der Oberen Elbe, an drei Fangstellen in der Mittelelbe und an fuenf Fangorten in der Tideelbe. Die Befunde wurden hinsichtlich regionaler Belastung und Vermarktungsfaehigkeit dargestellt. In dem vorliegenden Bericht werden ausnahmslos die Schadstoffgehalte der Brassenfaenge der Elbe des Jahres 1994 ausgewertet. Zur Festlegung der Hintergrundbelastung (Referenzstandard) des Bewertungssystems wurden Brassen aus voraussichtlich gering belasteten Gewaessern beruecksichtigt. Ziel der Datenauswertung ist eine Klassifizierung, welche die regionalspezifische Kontamination im Muskelfleisch der Elbe-Brassen widerspiegelt. (orig.)

  4. ICI 182,780 has agonistic effects and synergizes with estradiol-17 beta in fish liver, but not in testis

    OpenAIRE

    Power Deborah M; Teodósio Helena R; Condeça João B; Singh Pratap B; Pinto Patrícia; Canário Adelino

    2006-01-01

    Abstract Background ICI 182,780 (ICI) belongs to a new class of antiestrogens developed to be pure estrogen antagonists and, in addition to its therapeutic use, it has been used to knock-out estrogen and estrogen receptor (ER) actions in several mammalian species. In the present study, the effects and mechanism of action of ICI were investigated in the teleost fish, sea bream (Sparus auratus). Methods Three independent in vivo experiments were performed in which mature male tilapia (Oreochrom...

  5. 真鲷虹彩病毒实时定量PCR检测方法的建立与应用%Development and application of a real-time PCR assay for the detection of red-sea bream iridovirus

    Institute of Scientific and Technical Information of China (English)

    赵玉然; 岳志芹; 谭乐义; 刘荭; 赵巍; 梁成珠; 史秀杰; 徐彪; 朱来华; 何俊强

    2011-01-01

    以真鲷虹彩病毒(Red-sea bream iridovirus,RSIV)主要衣壳蛋白(Major capsid protein,MCP)的基因保守片段为靶序列,利用Primer Express 3.0软件设计定量PCR引物,建立了RSIV的SYBR Green I实时定量PCR检测方法.将RSIV MCP基因连接pMD18-T载体,构建重组质粒,经过梯度稀释后作为标准品,根据标准品拷贝数(X)与Ct值的关系绘制了标准曲线,为Ct=-3.184 1gX+40.270,相关系数R2=0.996 9.熔解曲线分析表明,定量PCR产物的Tm值为82.5℃.该方法的检测限为2.20×102拷贝/反应,对流行性造血器官坏死病毒、淋巴囊肿病毒、蛙病毒3、甲鱼虹彩病毒都没有扩增反应,具有特异性.利用该方法对84批海水鱼类(石鲽、大菱鲆、鲈鱼)进行检测,其中5批鱼样品感染RSIV,并利用标准曲线对病毒含量进行了定量分析.%A sensitive and specific SYBR Green Ⅰ real-time PCR assay for the detection of red-sea bream iridovirus (RSIV) was established.The real-time PCR primers were designed according to the conserved region of major capsid protein (MCP) gene by using Primer Express 3.0 software.The RSIV MCP gene was inserted into pMD18-T vector to construct the recombinant plasmid.The resulted plasmid was serially diluted and used as the standards.The relationship between plasmid copy number(X)and Ct value was described as a standard curve: Ct=-3.184 lgX+40.270(with an R2 value of 0.996 9).The detection limit of the assay was 2.20×102 virus copies per reaction.The assay showed specificity and could not be amplified with RSIV and epizootic haematopoietic necrosis virus (EHNV), lymphocystis disease virus (LCDV), frog virus 3 (FV 3) ,or soft-shelled turtle iridovirus (STIV).The Tm of the specific product was obtained as 82.5 ℃ through the melting curve analysis.This assay was applied in detecting whether the sea fish samples (stone flounder, turbot, and weever) of 84 batches were infected by RSIV.It was found that the samples of 5 batches presented positive

  6. Optimization of capillary electrophoresis-inductively coupled plasma mass spectrometry for species analysis of metallothionein-like proteins extracted from liver tissues of Elbe-bream and Roe deer

    Energy Technology Data Exchange (ETDEWEB)

    Proefrock, Daniel; Prange, Andreas E-mail: andreas.prange@gkss.de; Schaumloeffel, Dirk; Ruck, Wolfgang

    2003-08-15

    Species analysis of metallothionein-like proteins (MLP) in liver tissues from Elbe-Bream (Abramis brama L.) and Roe Deer (Capreolus capreolus L.) using capillary electrophoresis (CE) combined with inductively coupled plasma mass spectrometry detection is described. In order to allow systematic development of the method, commercially available metallothionein (MT) preparations of rabbit liver were used. Optimum separation efficiency was obtained by investigating the influence of parameters such as voltage, capillary temperature, buffer concentration, buffer pH and the use of different buffer systems. Instrumental parameters such as CE capillary position, interface adjustment and contamination problems are also discussed. Separation was performed using uncoated fused silica capillaries with 75 {mu}m i.d. and 70 cm length. The optimum conditions were found to be: Separation voltage 30 kV, positive polarity, capillary temperature 288.15 K and a buffer concentration of 100 mmol l{sup -1} Tricine-NH{sub 3} adjusted to pH 7.2. Sample preparation was performed so as to minimize oxidation and heavy metal contamination of the samples. The high molecular mass protein matrix was reduced by acetonitrile precipitation. For commercial MT preparations the relative standard deviations (R.S.D) in the retention times were 0.9% for MT-1 and 1.9% for MT-2; the R.S.D.'s in the peak areas were less than 6% for MT-1 and 16% for MT-2, respectively. Under optimized conditions the MLPs in the real samples could be separated efficiently in less than 10 min. By comparison with the migration times of commercially available MT preparations, two of the observed peaks could be assigned to MT-1 and MT-2.

  7. Optimization of capillary electrophoresis-inductively coupled plasma mass spectrometry for species analysis of metallothionein-like proteins extracted from liver tissues of Elbe-bream and Roe deer

    International Nuclear Information System (INIS)

    Species analysis of metallothionein-like proteins (MLP) in liver tissues from Elbe-Bream (Abramis brama L.) and Roe Deer (Capreolus capreolus L.) using capillary electrophoresis (CE) combined with inductively coupled plasma mass spectrometry detection is described. In order to allow systematic development of the method, commercially available metallothionein (MT) preparations of rabbit liver were used. Optimum separation efficiency was obtained by investigating the influence of parameters such as voltage, capillary temperature, buffer concentration, buffer pH and the use of different buffer systems. Instrumental parameters such as CE capillary position, interface adjustment and contamination problems are also discussed. Separation was performed using uncoated fused silica capillaries with 75 μm i.d. and 70 cm length. The optimum conditions were found to be: Separation voltage 30 kV, positive polarity, capillary temperature 288.15 K and a buffer concentration of 100 mmol l-1 Tricine-NH3 adjusted to pH 7.2. Sample preparation was performed so as to minimize oxidation and heavy metal contamination of the samples. The high molecular mass protein matrix was reduced by acetonitrile precipitation. For commercial MT preparations the relative standard deviations (R.S.D) in the retention times were 0.9% for MT-1 and 1.9% for MT-2; the R.S.D.'s in the peak areas were less than 6% for MT-1 and 16% for MT-2, respectively. Under optimized conditions the MLPs in the real samples could be separated efficiently in less than 10 min. By comparison with the migration times of commercially available MT preparations, two of the observed peaks could be assigned to MT-1 and MT-2

  8. Optimization of capillary electrophoresis?inductively coupled plasma mass spectrometry for species analysis of metallothionein-like proteins extracted from liver tissues of Elbe-bream and Roe deer

    Science.gov (United States)

    Pröfrock, Daniel; Prange, Andreas; Schaumlöffel, Dirk; Ruck, Wolfgang

    2003-08-01

    Species analysis of metallothionein-like proteins (MLP) in liver tissues from Elbe-Bream ( Abramis brama L.) and Roe Deer ( Capreolus capreolus L.) using capillary electrophoresis (CE) combined with inductively coupled plasma mass spectrometry detection is described. In order to allow systematic development of the method, commercially available metallothionein (MT) preparations of rabbit liver were used. Optimum separation efficiency was obtained by investigating the influence of parameters such as voltage, capillary temperature, buffer concentration, buffer pH and the use of different buffer systems. Instrumental parameters such as CE capillary position, interface adjustment and contamination problems are also discussed. Separation was performed using uncoated fused silica capillaries with 75 μm i.d. and 70 cm length. The optimum conditions were found to be: Separation voltage 30 kV, positive polarity, capillary temperature 288.15 K and a buffer concentration of 100 mmol l -1 Tricine-NH 3 adjusted to pH 7.2. Sample preparation was performed so as to minimize oxidation and heavy metal contamination of the samples. The high molecular mass protein matrix was reduced by acetonitrile precipitation. For commercial MT preparations the relative standard deviations (R.S.D) in the retention times were 0.9% for MT-1 and 1.9% for MT-2; the R.S.D.'s in the peak areas were less than 6% for MT-1 and 16% for MT-2, respectively. Under optimized conditions the MLPs in the real samples could be separated efficiently in less than 10 min. By comparison with the migration times of commercially available MT preparations, two of the observed peaks could be assigned to MT-1 and MT-2.

  9. Functional genomics in fish towards understanding stress and immune responses at a molecular level /

    OpenAIRE

    Ribas Cabezas, Laia

    2006-01-01

    Aquesta tesis doctoral està basada en estudiar la resposta immunològica dels peixos en models d'estrès i d'activació del sistema immune des la genòmica funcional. L'aplicació de tecnologies moleculars com el Differential Display van permetre identificar y clonar por primera vegada en orades (Sparus aurata) y en altres especies de peix, el gen enolasa. Aquest enzim glucolític s'ha plantejat per primera vegada com un bon marcador molecular per estudiar el benestar dels peixos. Per mitjà de l'ús...

  10. Studie van de biologie en de kweek van zeebaars Dicentrarchus labrax (L.) in intensieve aquacultuurcondities

    OpenAIRE

    Verdonck, W.

    1989-01-01

    In Europe. the interest in sea bass (Dicentrarchus labrax) as a culture fish is fast increasing. This results in a rapid expansion of sea bass culture and of that of other marine fish species. such as seabream (Sparus aurata) and turbot (Scophtalmus maximus). In 1983 a research project was started, with the aim of culturing sea bass in the thermal effluents of the nuclear power station at Doel. In this way the possibility of producing valuable proteins is created while at the same time therma...

  11. Mx Protein involvement in the innate immune system against viral infections in Senegalese Sole (Solea senegalensis)

    OpenAIRE

    Álvarez-Torres, Daniel

    2014-01-01

    La acuicultura es una actividad de gran interés económico y ecológico, que presenta un alto potencial de crecimiento, y cuyo desarrollo sostenible constituye la vía óptima de aprovechamiento de los recursos pesqueros sin perjuicio de las poblaciones naturales de las especies cultivadas. Hasta 1990 el cultivo de peces marinos en España estaba dominado por la dorada (Sparus aurata); sin embargo, la necesidad de diversificación ha llevado al cultivo de otras especies, entre las que destaca el le...

  12. FITOPLANKTONSKI SASTAV NA UZGAJALIŠTU RIBA U UVALI MASLINOVA (OTOK BRAČ)

    OpenAIRE

    SKEJIĆ, Sanda; Marasović, Ivona; NINČEVIĆ GLADAN, Živana

    2012-01-01

    Cilj ovog rada je utvrditi sastav fitoplanktona na uzgajalištu komarči (Sparus aurata) i lubina (Dicentrarchus labrax) u srednjem Jadranu. Istraživanje je provedeno u razdoblju od rujna 2005. do rujna 2006. godine na postaji smještenoj u uvali Maslinova na otoku Braču. Promatrajući cijelo istraživano razdoblje, dijatomeje su općenito prevladavale svojom brojnošću, dok su dinoflagelati bili brojniji u lipnju. Broj vrsta dijatomeja i dinoflagelata kroz istraživano razdoblje bio je sličan. Z...

  13. SASTAV FITOPLANKTONA NA UZGAJALIŠTU RIBA I ŠKOLJKAŠA U UVALI KALDONTA (OTOK CRES)

    OpenAIRE

    Tomec, M.

    2004-01-01

    Uvala Kaldonta nalazi se na jugozapadnoj obali otoka Cresa u Lošinjskom kanalu na području bez većeg utjecaja opće morske struje. U uvali su postavljena 44 plutajuća kaveza veličine 5x10 metara u kojima se uspješno proizvodi oko 70 tona morske ribe: komarča (Sparus aurata), lubin (Dicentrarchus labrax), pic (Diplodus puntazzo) i zubatac (Dentex dentex). Uz neke fizikalnokemijske pokazatelje (temperatura mora, prozirnost, salinitet), posebna je pozornost posvećena kvalitativnom sastavu mrežnog...

  14. Contribuição de decavanadato para os efeitos IN VIVO e IN VITRO de vanadato no músculo cardíaco

    OpenAIRE

    Soares, S. S.

    2007-01-01

    Tese dout., Biologia - Fisiologia Animal, Universidade do Algarve, 2007 No presente estudo aborda-se a contribuição da espécie decamérica de vanadato nos efeitos tóxicos in vivo e in vitro de vanadato. Após administração intravenosa de decavanadato em Sparus aurata, o vanadato decamérico induz respostas nos marcadores de stresse oxidativo do tecido cardíaco distintas das promovidas pelo monovanadato, evidenciando a importância de considerar a contribuição dos diferentes olig...

  15. Effect of cysteamine hydrochloride and luteininzing hormone-releasing hormone analog on the growth and the expression of IGF-Ⅰ mRNA in the yellow-fin porgy Sparus latus%半胱胺盐酸盐和LHRH-A对黄鳍鲷IGF-Ⅰ基因表达和生长的影响

    Institute of Scientific and Technical Information of China (English)

    石和荣; 张勇; 张为民; 刘晓春; 柯浩; 林浩然

    2005-01-01

    This paper was focused on the effects of cysteamine (CSH) and luteininzing hormone-releasing hormone analog (LHRH-A, D-Ala6, Pro9-Net-LHRH) on the expression of IGF-Ⅰ mRNA and the growth in juvenile yellow-fin porgy Sparus latus.IGF-ⅠcDNA of yellow-fin porgy was cloned from the liver using GeneRacerIM kit (Invitrogen), The IGF-Ⅰ cDNA is 840 bp in length, and the open reading frame encodes a putative protein of 185 amino acids.The comparison of the deduced amino acid sequence indicated that yellow-fin porgy IGF-Ⅰshared 75.2% identity with zebrafish, 75.8% with goldfish, 84.7% with trout, 86.5% with turbot, 96.8% with grouper, and 100% with seabream.The analysis of the E domain showed that porgy IGF-Ⅰ belonged to type Ea-4.The experiments were conducted from August to October 2003 for 45 days and the effects of CSH and LHRH-A administered in the diet on GH, growth, IGF-Ⅰ mRNA were determined.Healthy juvenile yellow-fin porgy (body weight 8.9±2.5 g) were maintained in outdoor cages (15 m×1.5 m×1.2 m) in seawater under natural photoperiod.Water temperatures varied from 23 to 28° C.and the dissolved oxygen, pH and salinity were around 6.0 mg/L, 8.0 and 10‰, respectively.After acclimation of two weeks, the yellow-fin porgy were randomly and equally allocated to cages and fed formulated diet twice a day to satiation.The IGF-ⅠmRNA expression in the liver was examined with semi-quantitative RT-PCR, and GH concentrations in the pituitary were assayed with heterogenous RIA.The results showed that CSH (1.25 mg/g diet) and LHRH-A (10 μg/g diet) alone or in combination significantly increased the pituitary GH contents and the hepatic IGF- I mRNA levels of yellow-fin porgy as compared to that of the control group at the end of the experiment, The relative somatic growth rates (RSGR) of yellow-fin porgy fed with CSH and LHRH-A alone or in combination for 45 days were significantly higher than those of the control group.The relative linear growth rates (RLGR

  16. Chitosan-Mediated shRNA Knockdown of Cytosolic Alanine Aminotransferase Improves Hepatic Carbohydrate Metabolism.

    Science.gov (United States)

    González, Juan D; Silva-Marrero, Jonás I; Metón, Isidoro; Caballero-Solares, Albert; Viegas, Ivan; Fernández, Felipe; Miñarro, Montserrat; Fàbregas, Anna; Ticó, Josep R; Jones, John G; Baanante, Isabel V

    2016-02-01

    Alanine aminotransferase (ALT) catalyses a transamination reaction that links carbohydrate and amino acid metabolism. In this study, we examined the effect of silencing cytosolic ALT (cALT) expression on the hepatic metabolism in Sparus aurata. A number of siRNA and shRNA designed to down-regulate cALT expression were validated in HEK-293 cells transfected with plasmids expressing S. aurata cALT or mitochondrial ALT (mALT) isoforms: ALT silencing significantly decreased the expression levels of S. aurata mRNA cALT1 to 62% (siRNA) and 48% (shRNA) of the values observed in control cells. The effect of cALT silencing was analysed in the liver of S. aurata 72 h after intraperitoneal injection of chitosan-tripolyphosphate (TPP) nanoparticles complexed with a plasmid encoding a shRNA to down-regulate cALT expression (pCpG-si1sh1). In fish fed diets with different ratio of protein to carbohydrate and treated with chitosan-TPP-pCpG-si1sh1, cALT1 and cALT2 mRNA levels significantly decreased irrespective of the diet. Consistently, ALT activity decreased in liver of treated animals. In the liver of S. aurata treated with chitosan-TPP-pCpG-si1sh1 nanoparticles, down-regulation of cALT expression increased the activity of key enzymes in glycolysis (6-phosphofructo-1-kinase and pyruvate kinase) and protein metabolism (glutamate dehydrogenase). Besides showing for the first time that administration of chitosan-TPP-pCpG-si1sh1 nanoparticles silences hepatic cALT expression in vivo, our data support that down-regulation of cALT could improve the use of dietary carbohydrates to obtain energy and spare protein catabolism. PMID:26475146

  17. Inmunotoxicología producida por metales pesados y caracterización del moco de piel en peces= Heavy metal immunotoxicology and skin mucus in fish

    OpenAIRE

    Guardiola Abellán, Francisco Antonio

    2014-01-01

    Durante la presente Tesis Doctoral se han estudiado los efectos inmunotoxicológicos de la exposición mediante baño a arsénico, cadmio y mercurio en la dorada (Sparus aurata L.), la cual es una especie que posee la mayor tasa de producción en la acuicultura mediterránea. Por otra parte, también se han estudiado los parámetros inmunológicos y físico-químicos del moco de la piel de cinco especies de peces teleósteos marinos. En el caso de la dorada los parámetros inmunológicos en el moco se comp...

  18. Dicty_cDB: Contig-U12708-1 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available nii ATCC 3340... 74 3e-12 (Q8N0X2) RecName: Full=Sperm-associated antigen 16 protein; AltN... 74 3e-...anin mR... 71 2e-11 (Q7ZXZ2) RecName: Full=U3 small nucleolar RNA-associated... nucleot... 78 2e-13 BC158602_1( BC158602 |pid:none) Rattus norvegicus sperm associated... 78 2e-13...ifera contig VV78X121538.... 74 3e-12 BC025379_1( BC025379 |pid:none) Homo sapiens sperm associated anti... 74 3e-...11 DQ111989_1( DQ111989 |pid:none) Sparus aurata activated protein ki... 72 1e-11 AE017347_451( AE017347 |pid:none) Cryptococc

  19. Are combined AOPs effective for toxicity reduction in receiving marine environment? Suitability of battery of bioassays for wastewater treatment plant (WWTP) effluent as an ecotoxicological assessment.

    Science.gov (United States)

    Díaz-Garduño, B; Rueda-Márquez, J J; Manzano, M A; Garrido-Pérez, C; Martín-Díaz, M L

    2016-03-01

    Ecotoxicological assessment of three different wastewater treatment plant (WWTP) effluents D1, D2 and D3 was performed before and after tertiary treatment using combination of advanced oxidation processes (AOPs). A multibarrier treatment (MBT) consisting of microfiltration (MF), hydrogen peroxide photolysis (H2O2/UVC) and catalytic wet peroxide oxidation (CWPO) was applied for all effluents. Sparus aurata, Paracentrotus lividus, Isochrysis galbana and Vibrio fischeri, representing different trophic levels, constituted the battery of bioassays. Different acute toxicity effects were observed in each WWTP effluents tested. The percentage of sea urchin larval development and mortality fish larvae were the most sensitive endpoints. Significant reduction (p effluent's toxicity was observed using a classification pT-method after MBT process. Base on obtained results, tested battery of bioassays in pT-method framework can be recommended for acute toxicity preliminary evaluation of WWTP effluents for the marine environment. PMID:26741736

  20. A scanning electron microscopy study of Argulus vittatus (Rafinesque-Schmaltz, 1814) (Crustacea: Branchiura) from Algerian coast.

    Science.gov (United States)

    Ider, Djamila; Ramdane, Zouhir; Courcot, Lucie; Amara, Rachid; Trilles, Jean-Paul

    2014-06-01

    A study of the Algerian Branchiura, Argulus vittatus (Rafinesque-Schmaltz, 1814) was conducted using the scanning electron microscopy (SEM). New morphological features are reported for the first time (mouth con, first maxilla, second maxilla, structures and ornamentation of thoracic segments, structure of semen papillae, etc.). The morphology of small and large female specimens was compared. Two new hosts, Pagellus erythrinus L. and Sparus aurata L., are reported for this species. Until now, six host species were reported for A. vittatus, and stenoxenic specificity for Sparid fishes was observed for Algerian specimens. The biogeographical distribution of this species seems to be restricted to the eastern coast of Algeria. Additional information about the host specificity, ecology, and life cycle of this parasitic species were given. PMID:24728522

  1. Comparison of morphology parameters of blunt snout bream (Megalobrama amblycephala Yih) among Liangzi Lake,Poyang Lake and Yuni Lake%梁子湖、鄱阳湖和淤泥湖团头鲂的形态学比较

    Institute of Scientific and Technical Information of China (English)

    曾聪; 阎里清; 高泽霞; 曹小娟; 钱雪桥; 王卫民

    2012-01-01

    Blunt snout bream (Megalobrama amblycephala) is a widespread and one of the main aquaculture species in China. In present study,through univariate and multivariate analysis,we compared the external morphology of M. Amblycephala from three different locations (Liangzi,Poyang and Yuni Lake). The results from one-way ANOVA and one-way analysis of covariance showed significant differences (P<0. 05) in all the morphological parameters among the three populations, except for DRC, CRC,HD, DL, AL, PRPD and ND. We observed two discriminant functions among M. Amblycephala from three lakes and these were more accurate between Yuni and Liangzi populations. In the principal component analysis (PCA), three principal components were constructed and the related contribution ratios were 39. 78%,22. 63% and 6. 53%,respectively,with the cumulative contribution ratio of 68. 95%. The multivariate analysis indicated that morphological variation between Liangzi and Yuni populations was greater, while Liangzi and Poyang populations were more similar in morphology. According to Mayr's 75% rule, the differences among these three populations were still within the population level.%对团头鲂的3个种质资源区(梁子湖、鄱阳湖和淤泥湖)群体的可量性状和可数性状进行了多元统计分析.对可数性状的方差分析结果表明,3个团头鲂群体在侧线鳞数、侧线上鳞数、侧线下鳞数、胸鳍鳍条数以及臀鳍鳍条数上存在显著性差异;以体长作为协变量对可量性状进行的协方差分析显示,3个团头鲂群体在全长、体高、头长、头宽、尾柄长、尾柄高、背前区长和腹鳍前长存在显著性差异.对可量性状建立判别函数以及对可量性状平均值进行聚类分析表明梁子湖团头鲂与鄱阳湖团头鲂在外部形态上更为相似.主成分分析构建了3个主成分,其贡献率分别为39.78%、22.63%和6.53%,累计贡献率为68.95%.多元统计分析显示梁子湖群体

  2. Haematological characteristics associated with parasitism in bream, Abramis brama orientalis

    OpenAIRE

    Hayatbakhsh, Mohammad Reza; Khara, Hossein; Movahed, Rashideh; Sayadborani, Mohammad; Rohi, Javad Daghigh; Ahmadnezhad, Mohadesseh; Rahbar, Mina; Rad, Amir Sajedi

    2013-01-01

    A parasitological investigation was done on 175 specimens. Infections of A. brama orientalis were analyzed according to the age and sex. The fish also were examined for evaluation changes of haematological parameters in relation to parasitic infection. Four parasites were found, including—Caryophyllaeus laticeps and Ligula intestinalis (Cestoda), Diplostomum spathaceum (Platyhelminthes) and Trichodina sp. (Ciliophora). Among identified parasites maximum prevalence and mean intensity were rela...

  3. Haematological characteristics associated with parasitism in bream, Abramis brama orientalis.

    Science.gov (United States)

    Hayatbakhsh, Mohammad Reza; Khara, Hossein; Movahed, Rashideh; Sayadborani, Mohammad; Rohi, Javad Daghigh; Ahmadnezhad, Mohadesseh; Rahbar, Mina; Rad, Amir Sajedi

    2014-12-01

    A parasitological investigation was done on 175 specimens. Infections of A. brama orientalis were analyzed according to the age and sex. The fish also were examined for evaluation changes of haematological parameters in relation to parasitic infection. Four parasites were found, including-Caryophyllaeus laticeps and Ligula intestinalis (Cestoda), Diplostomum spathaceum (Platyhelminthes) and Trichodina sp. (Ciliophora). Among identified parasites maximum prevalence and mean intensity were related to Ligula intestinalis and Caryophyllaeus laticeps respectively. The values of prevalence and mean intensity showed significant differences among ages. Our results revealed prevalence, mean intensity and abundance had not significant difference between males and females. Parasite infection provoked reduction (P < 0.05) in haematocrit, mean cell volume and lymphocyte. On the other hand, significant increase (P < 0.05) in white blood cell (WBC), mean cell haemoglobin concentration and neutrophil in blood of infected fish was observed. Significant differences were detected for the WBC, lymphocyte and neutrophil (infected versus uninfected by Trichodina sp., Diplostomum spathaceum and Caryophyllaeus laticeps). In addition to WBC and lymphocytes, significant change was observed for the haemoglobin (Hb) (infected versus uninfected by Ligula intestinalis). PMID:25320488

  4. Emerging pathogens of gilthead seabream: characterisation and genomic analysis of novel intracellular β-proteobacteria.

    Science.gov (United States)

    Seth-Smith, Helena M B; Dourala, Nancy; Fehr, Alexander; Qi, Weihong; Katharios, Pantelis; Ruetten, Maja; Mateos, José M; Nufer, Lisbeth; Weilenmann, Roseline; Ziegler, Urs; Thomson, Nicholas R; Schlapbach, Ralph; Vaughan, Lloyd

    2016-07-01

    New and emerging environmental pathogens pose some of the greatest threats to modern aquaculture, a critical source of food protein globally. As with other intensive farming practices, increasing our understanding of the biology of infections is important to improve animal welfare and husbandry. The gill infection epitheliocystis is increasingly problematic in gilthead seabream (Sparus aurata), a major Mediterranean aquaculture species. Epitheliocystis is generally associated with chlamydial bacteria, yet we were not able to localise chlamydial targets within the major gilthead seabream lesions. Two previously unidentified species within a novel β-proteobacterial genus were instead identified. These co-infecting intracellular bacteria have been characterised using high-resolution imaging and genomics, presenting the most comprehensive study on epitheliocystis agents to date. Draft genomes of the two uncultured species, Ca. Ichthyocystis hellenicum and Ca. Ichthyocystis sparus, have been de novo sequenced and annotated from preserved material. Analysis of the genomes shows a compact core indicating a metabolic dependency on the host, and an accessory genome with an unprecedented number of tandemly arrayed gene families. This study represents a critical insight into novel, emerging fish pathogens and will be used to underpin future investigations into the bacterial origins, and to develop diagnostic and treatment strategies. PMID:26849311

  5. Cloning and Tissue Expression Analysis ofPAX7 Gene in Grass Carp (Ctenopharyngodon idellus)%草鱼PAX7基因的克隆、序列分析及组织表达

    Institute of Scientific and Technical Information of China (English)

    甘甜; 冷向军; 郭婷; 胡静; 魏静; 李小勤

    2014-01-01

    Paired box gene 7(PAX7)is crucially important to the cellular renewal, differentiation and apoptosis, especially in neural crest development, gastrulation, and muscle self-renewal. ThePAX7 domains sequence is conserved among several species, such as zebrafish, apteronotidae, and rainbow trout, and the conserved sequence zebrafish was used to design degenerate primers for reverse-transcription PCR(RT-PCR). A partial sequence ofPAX7 from grass carp was obtained for a 645 bp segment encoding a 214 amino acid peptide, containing a paired box domain with 128 amino acids. The deduced protein showed homology to zebrafish(Danio rerio), apteronotidae(Sternopygus macrurus), Japanese Medaka(Oryzias latipes), gilthead bream(Sparus aurata), rainbow trout(Oncorhynchus mykiss), Atlantic salmon(Salmo salar), Arctic charr(Salvelinus alpinus), human(Homo sapiens), wild yak(Bos grunniens mutus), brown rat(Rattus norvegicus), and mouse(Mus musculus)with 90%-97% identities. Analysis of the PAX7 phylogenetic tree revealed that the grass carp joined with zebrafish, and there was a confluence of Japanese medaka, Arctic charr, Atlantic salmon, rainbow trout, apteronotidae and gilthead bream. The other branch was consisted of mouse, brown rat, wild yak and human. These results conformed to the traditional species classification evolution status. The expression in tissues was detected by Real-time quantitative PCR, which indicated that the highest level ofPAX7 gene expression was found in muscle, a lower expression level was found in foregut and skin, and the lowest detectable level was found in heart, brain, kidney and liver. The results are in agreement with the function of the gene.%配对盒基因7(Paired box 7 gene,PAX7)在神经嵴发育及原肠胚形成、肌肉自主更新与再生中扮演着重要角色,对细胞更新、分化、凋亡等起着十分重要的调控作用。参照GenBank中斑马鱼、线鳍电鳗和虹鳟等物种PAX7序列的保守区域设计简并引

  6. Rapid determination of vitellogenin in fish plasma by anion exchange high performance liquid chromatography using postcolumn fluorescence derivatization with o-phthalaldehyde.

    Science.gov (United States)

    Wu, Cuiqin; Yuan, Dongxing; Liu, Baomin

    2006-12-01

    An analytical method involving anion exchange high performance liquid chromatographic determination of vitellogenin (Vtg) in fish plasma after postcolumn fluorescence derivatization with o-phthalaldehyde (OPA) was developed. The retention time of Vtg was about 11 min. The reagent variables for derivatization were optimized. The fluorophore was excited at 335 nm and detected at 435 nm. A calibration curve was established ranging from 0.13 to 11.28 microg. The determination limit of Vtg was found to be as low as 0.13 microg. The spiked recovery was 93.6% and interassay variability was less than 4%. The method developed was used to determine Vtg in fish plasma obtained from red sea bream (Pagrosomus major), black porgy (Sparus macrocephalus) and skew band grunt (Hapalogenys nitens), without complicated sample pretreatment. The results confirmed that the method showed advantages of being simple, rapid, reproducible and sensitive. PMID:17159322

  7. Immunohistochemical detection of estrogen receptors in fish scales.

    Science.gov (United States)

    Pinto, P I S; Estêvão, M D; Redruello, B; Socorro, S M; Canário, A V M; Power, D M

    2009-01-01

    Calcium mobilization from internal stores, such as scales, induced by 17beta-estradiol during sexual maturation in salmonids is well documented. This calcium mobilization from scales is proposed to be mediated by the estrogen receptor (ER). However, the ER subtypes involved and signaling mechanisms responsible for this effect remain to be fully characterized. In the present study, we have localized ERalpha, ERbetaa and ERbetab proteins in juvenile and adult sea bream (Sparus auratus) and Mozambique tilapia (Oreochromis mossambicus) scales by immunohistochemistry with sea bream ER subtype specific antibodies. The three ERs were detected in isolated or small groups of round cells, in the basal layer of the scales of both juvenile and adult fish and the localization and signal intensity varied with the species and age of the animals. The ERs may be co-localized in cells of the scale posterior region that expressed tartrate-resistant acid phosphatase (TRAP), a marker for osteoclasts. These results suggest that the calcium mobilizing action of 17beta-estradiol on fish scales is via its direct action on ERs localized in osteoclasts. PMID:18977356

  8. Isolation of a novel aquaglyceroporin from a marine teleost (Sparus auratus): function and tissue distribution.

    NARCIS (Netherlands)

    Santos, C.R.; Estevao, M.D.; Fuentes, J.; Cardoso, J.C.; Fabra, M.; Passos, A.L.; Detmers, F.J.M.; Deen, P.M.T.; Cerda, J.; Power, D.M.

    2004-01-01

    The aquaporins (formerly called the major intrinsic protein family) are transmembrane channel proteins. The family includes the CHIP group, which are functionally characterised as water channels and the GLP group, which are specialised for glycerol transport. The present study reports the identifica

  9. Calcium mobilization from fish scales is mediated by parathyroid hormone related protein via the parathyroid hormone type 1 receptor.

    Science.gov (United States)

    Rotllant, J; Redruello, B; Guerreiro, P M; Fernandes, H; Canario, A V M; Power, D M

    2005-12-15

    The scales of bony fish represent a significant reservoir of calcium but little is known about their contribution, as well as of bone, to calcium balance and how calcium deposition and mobilization are regulated in calcified tissues. In the present study we report the action of parathyroid hormone-related protein (PTHrP) on calcium mobilization from sea bream (Sparus auratus) scales in an in vitro bioassay. Ligand binding studies of piscine 125I-(1-35(tyr))PTHrP to the membrane fraction of isolated sea bream scales revealed the existence of a single PTH receptor (PTHR) type. RT-PCR of fish scale cDNA using specific primers for two receptor types found in teleosts, PTH1R, and PTH3R, showed expression only of PTH1R. The signalling mechanisms mediating binding of the N-terminal amino acid region of PTHrP were investigated. A synthetic peptide (10(-8) M) based on the N-terminal 1-34 amino acid residues of Fugu rubripes PTHrP strongly stimulated cAMP synthesis and [3H]myo-inositol incorporation in sea bream scales. However, peptides (10(-8) M) with N-terminal deletions, such as (2-34), (3-34) and (7-34)PTHrP, were defective in stimulating cAMP production but stimulated [3H]myo-inositol incorporation. (1-34)PTHrP induced significant osteoclastic activity in scale tissue as indicated by its stimulation of tartrate-resistant acid phosphatase. In contrast, (7-34)PTHrP failed to stimulate the activity of this enzyme. This activity could also be abolished by the adenylyl cyclase inhibitor SQ-22536, but not by the phospholipase C inhibitor U-73122. The results of the study indicate that one mechanism through which N-terminal (1-34)PTHrP stimulates osteoclastic activity of sea bream scales, is through PTH1R and via the cAMP/AC intracellular signalling pathway. It appears, therefore, that fish scales can act as calcium stores and that (1-34)PTHrP regulates calcium mobilization from them; it remains to be established if this mechanism contributes to calcium homeostasis in vivo

  10. ICI 182,780 has agonistic effects and synergizes with estradiol-17 beta in fish liver, but not in testis

    Directory of Open Access Journals (Sweden)

    Power Deborah M

    2006-12-01

    Full Text Available Abstract Background ICI 182,780 (ICI belongs to a new class of antiestrogens developed to be pure estrogen antagonists and, in addition to its therapeutic use, it has been used to knock-out estrogen and estrogen receptor (ER actions in several mammalian species. In the present study, the effects and mechanism of action of ICI were investigated in the teleost fish, sea bream (Sparus auratus. Methods Three independent in vivo experiments were performed in which mature male tilapia (Oreochromis mossambicus or sea bream received intra-peritoneal implants containing estradiol-17 beta (E2, ICI or a combination of both compounds. The effects of E2 and ICI on plasma calcium levels were measured and hepatic and testicular gene expression of the three ER subtypes, ER alpha, ER beta a and ER beta b, and the estrogen-responsive genes, vitellogenin II and choriogenin L, were analyzed by semi-quantitative RT-PCR in sea bream. Results E2 treatment caused an increase in calcium levels in tilapia, while ICI alone had no noticeable effect, as expected. However, pretreatment with ICI synergistically potentiated the effect of E2 on plasma calcium in both species. ICI mimicked some E2 actions in gene expression in sea bream liver upregulating ER alpha, vitellogenin II and choriogenin L, although, unlike E2, it did not downregulate ER beta a and ER beta b. In contrast, no effects of E2 or ICI alone were detected in the expression of ERs in testis, while vitellogenin II and choriogenin L were upregulated by E2 but not ICI. Finally, pretreatment with ICI had a synergistic effect on the hepatic E2 down-regulation of ER beta b, but apparently blocked the ER alpha up-regulation by E2. Conclusion These results demonstrate that ICI has agonistic effects on several typical estrogenic responses in fish, but its actions are tissue-specific. The mechanisms for the ICI agonistic activity are still unknown; although the ICI induced up-regulation of ER alpha mRNA could be one of

  11. Development of a convenience and safety chilled sous vide fish dish: Diversification of aquacultural products.

    Science.gov (United States)

    Espinosa, M C; López, G; Díaz, P; Linares, M B; Garrido, M D

    2016-04-01

    The dynamic expansion of the ready-to-eat seabream sector in its adaptation to new lifestyles has led to the search for new presentation formats in seabream (Sparus aurata). Green sauce (olive oil, wine vinegar, garlic, fresh parsley, black pepper, basil and salt) and 60 ℃ of cooking temperature were chosen by the panellists for the sous vide cooking process. Seabream fillet and sauce were packaged in polypropylene trays, cooked, chilled and stored at 2 ℃. Microbiological (total viable counts,Enterobacteriaceae,lactic acid bacteria, anaerobic psychrotrophic, moulds and yeasts, Salmonella and Listeria monocytogenes), chemical (pH and TBARs) and sensory parameters were determined at 0, 7, 17, 34, 48 and 62 days. In the conditions used, the microbiological counts remained stable, and Salmonella and Listeria monocytogenes were absent. The acidic sauce had a positive effect on the pH of the product, and low TBARs were obtained throughout storage. The processing conditions used in the present study allowed a chilled ready-to-eat seabream product of consistently high quality up to 62 days of storage to be obtained, representing an expansion of the products offered by the aquacultural industry. PMID:25941212

  12. State of the art of Italian aquaculture

    Directory of Open Access Journals (Sweden)

    P. Melotti

    2010-04-01

    Full Text Available According to aquaculture production statistics published by FEAP (2007, Italy is the fifth largest fish producer in the European Union having a total quantity for 2006 estimated around 60,000 t. This data is exclusively referred to rainbow trout (Oncorhynchus mykiss, seabream (Sparus aurata, seabass (Dicentrarchus labrax and European eel (Anguilla anguilla but even if we consider the total aquaculture production elaborated by ISMEA (2006 through year 2005 including sturgeons, carps, striped bass, catfish and ornamental fish, Italy ranks fourth with 69,100 t after Norway (655,364 t, Great Britain (141,793 t and Greece (83,600 t. Over the last 15 years, Italian finfish production has known a decrease related to all the species mainly reared (trout, eel, carps, catfish except for the eurhyaline species that have had an important expansion (Table 1. Based on these considerations, in this work we describe the main features of fish aquaculture in Italy focalizing the attention to the single sectors of the farmed species and their trend for the future.

  13. Assessment of Tools for Marker-Assisted Selection in a Marine Commercial Species: Significant Association between MSTN-1 Gene Polymorphism and Growth Traits

    Directory of Open Access Journals (Sweden)

    Irma Sánchez-Ramos

    2012-01-01

    Full Text Available Growth is a priority trait from the point of view of genetic improvement. Molecular markers linked to quantitative trait loci (QTL have been regarded as useful for marker-assisted selection in complex traits as growth. Polymorphisms have been studied in five candidate genes influencing growth in gilthead seabream (Sparus aurata: the growth hormone (GH, insulin-like growth factor-1 (IGF-1, myostatin (MSTN-1, prolactin (PRL, and somatolactin (SL genes. Specimens evaluated were from a commercial broodstock comprising 131 breeders (from which 36 males and 44 females contributed to the progeny. In all samples eleven gene fragments, covering more than 13,000 bp, generated by PCR-RFLP, were analyzed; tests were made for significant associations between these markers and growth traits. ANOVA results showed a significant association between MSTN-1 gene polymorphism and growth traits. Pairwise tests revealed several RFLPs in the MSTN-1 gene with significant heterogeneity of genotypes among size groups. PRL and MSTN-1 genes presented linkage disequilibrium. The MSTN-1 gene was mapped in the centromeric region of a medium-size acrocentric chromosome pair.

  14. Heavy metals produce toxicity, oxidative stress and apoptosis in the marine teleost fish SAF-1 cell line.

    Science.gov (United States)

    Morcillo, Patricia; Esteban, María Á; Cuesta, Alberto

    2016-02-01

    The use of cell lines to test the toxicity of aquatic pollutants is a valuable alternative to fish bioassays. In this study, fibroblast SAF-1 cells from the marine gilthead seabream (Sparus aurata L.) were exposed for 24 h to the heavy metals Cd, Hg, MeHg (Methylmercury), As or Pb and the resulting cytotoxicity was assessed. Neutral red (NR), MTT-tetrazolio (MTT), crystal violet (CV) and lactate dehydrogenase (LDH) viability tests showed that SAF-1 cells exposed to the above heavy metals produced a dose-dependent reduction in the number of viable cells. Methylmercury showed the highest toxicity (EC50 = 0.01 mM) followed by As, Cd, Hg and Pb. NR was the most sensitive method followed by MTT, CV and LDH. SAF-1 cells incubated with each of the heavy metals also exhibited an increase in the production of reactive oxygen species and apoptosis cell death. Moreover, the corresponding gene expression profiles pointed to the induction of the metallothionein protective system, cellular and oxidative stress and apoptosis after heavy metal exposure for 24 h. This report describes and compares tools for evaluating the potential effects of marine contamination using the SAF-1 cell line. PMID:26363324

  15. Impairment of mitochondrial energy metabolism of two marine fish by in vitro mercuric chloride exposure

    International Nuclear Information System (INIS)

    Highlights: • Hg levels usually found in contaminated environments may compromise fish metabolism. • Hg impairs both the oxidative phosphorylation and the electron transport chain. • The decrease of F1Fo-ATPase activity confirmed Hg inhibition of ADP phosphorylation. • The decrease of SDH activity supported Hg inhibition of the electron transport chain. • Mitochondrial respiration is a suitable biomarker of exposure to Hg. - Abstract: The goal of this work was to understand the extent of mercury toxic effects in liver metabolism under an episode of acute contamination. Hence, the effects of in vitro mercuric chloride in liver mitochondria were assessed in two commercial marine fish: Senegalese sole (Solea senegalensis) and gilthead seabream (Sparus aurata). Liver mitochondria were exposed to 0.2 mg L−1 of mercury, the average concentration found in fish inhabiting contaminated areas. Mercuric chloride depressed mitochondrial respiration state 3 and the maximal oxygen consumption in the presence of FCCP indicating inhibitory effects on the oxidative phosphorylation and on the electron transport chain, respectively. The inhibition of F1Fo-ATPase and succinate-dehydrogenase activities also corroborated the ability of mercury to inhibit ADP phosphorylation and the electron transport chain. This study brings new understanding on the mercury levels able to impair fish mitochondrial function, reinforcing the need for further assessing bioenergetics as a proxy for fish health status

  16. Some organochlorine pesticide residues in fish species in Konya, Turkey.

    Science.gov (United States)

    Kalyoncu, Leyla; Agca, Ihsan; Aktumsek, Abdurrahman

    2009-02-01

    The levels of organochlorine pesticides were measured in 18 fish species from Konya markets, Turkey. These species were selected on the basis of their importance to local human fish consumption. The extracted residues were analyzed on a micro capillary gas chromatograph equipped with an electron capture detector. Total 14 different organochlorine pesticides were determined. These residues were detected in all fish species, except in trout, horse mackerel and bonito. DDT and its metabolites and HCH were the predominant contaminants in fish muscles. The mean concentrations of summation operator DDT were in the range between 0.0008 and 0.0828 microg g(-1). DDT was the predominant residue in Sparus aurata. Detectable levels of HCH, aldrin, and heptachlor were found in most samples. However, dieldrin, endrin, beta endosulfan, p-p' DDT, and p-p' DDD were not found in Salmo trutta. The mean of endrin ranged from 0.0040 microg g(-1) (Triglia lineate) to 0.0326 microg g(-1) (Trachurus trachurus). These results give no indication of important health risks associated with the consumption of these fishes in Konya markets. PMID:19103455

  17. The Effect of 17α-Ethynylestradiol on Steroidogenesis and Gonadal Cytokine Gene Expression Is Related to the Reproductive Stage in Marine Hermaphrodite Fish

    Directory of Open Access Journals (Sweden)

    Isabel Cabas

    2013-12-01

    Full Text Available Pollutants have been reported to disrupt the endocrine system of marine animals, which may be exposed through contaminated seawater or through the food chain. Although 17α-ethynylestradiol (EE2, a drug used in hormone therapies, is widely present in the aquatic environment, current knowledge on the sensitivity of marine fish to estrogenic pollutants is limited. We report the effect of the dietary intake of 5 µg EE2/g food on different processes of testicular physiology, ranging from steroidogenesis to pathogen recognition, at both pre-spermatogenesis (pre-SG and spermatogenesis (SG reproductive stages, of gilthead seabream (Sparus aurata L., a marine hermaphrodite teleost. A differential effect between pre-SG and SG specimens was detected in the sex steroid serum levels and in the expression profile of some steroidogenic-relevant molecules, vitellogenin, double sex- and mab3-related transcription factor 1 and some hormone receptors. Interestingly, EE2 modified the expression pattern of some immune molecules involved in testicular physiology. These differences probably reflect a developmental adjustment of the sensitivity to EE2 in the gilthead seabream gonad.

  18. Sexual ornamentation and parasite infection in males of common bream (Abramis brama)

    Czech Academy of Sciences Publication Activity Database

    Ottová, E.; Šimková, A.; Jurajda, Pavel; Gelnar, M.

    Tallinn: Estonian Marine Institute, 2004 - (Saat, T.). s. 65 ISBN 9985-4-0396-7. ISSN 1406-023X. [European Congress of Ichthyology /11./. 06.09.2004-10.09.2004, Tallinn] Institutional research plan: CEZ:AV0Z6093917 Keywords : Abramis brama * parasites Subject RIV: EG - Zoology

  19. The food of common bream (Abramis brama L.) in a biomanipulated water supply reservoir

    Czech Academy of Sciences Publication Activity Database

    Zapletal, T.; Mareš, J.; Jurajda, Pavel; Všetičková, Lucie

    2012-01-01

    Roč. 60, č. 6 (2012), s. 357-366. ISSN 1211-8516 Institutional support: RVO:68081766 Keywords : diet * Abramis brama * impoundment * biomanipulation Subject RIV: EG - Zoology http://www.mendelu.cz/dok_server/slozka.pl?id=57208;download=103037

  20. Identification of MicroRNA for Intermuscular Bone Development in Blunt Snout Bream (Megalobrama amblycephala

    Directory of Open Access Journals (Sweden)

    Shi-Ming Wan

    2015-05-01

    Full Text Available Intermuscular bone (IB, which occurs only in the myosepta of the lower teleosts, is attracting more attention of researchers due to its particular development and lack of genetic information. MicroRNAs (miRNAs are emerging as important regulators for biological processes. In the present study, miRNAs from IBs and connective tissue (CT; encircled IBs from six-month-old Megalobrama amblycephala were characterized and compared. The results revealed the sequences and expression levels of 218 known miRNA genes (belonging to 97 families. Of these miRNAs, 44 known microRNA sequences exhibited significant expression differences between the two libraries, with 24 and 20 differentially-expressed miRNAs exhibiting higher expression in the CT and IBs libraries, respectively. The expressions of 11 miRNAs were selected to validate in nine tissues. Among the high-ranked predicted gene targets, differentiation, cell cycle, metabolism, signal transduction and transcriptional regulation were implicated. The pathway analysis of differentially-expressed miRNAs indicated that they were abundantly involved in regulating the development and differentiation of IBs and CT. This study characterized the miRNA for IBs of teleosts for the first time, which provides an opportunity for further understanding of miRNA function in the regulation of IB development.

  1. Effects of sea bass and sea bream farming (Western Mediterranean Sea) on peracarid crustacean assemblages

    OpenAIRE

    Fernandez-Gonzalez, Victoria; Sanchez-Jerez, Pablo

    2011-01-01

    Benthic soft–bottom assemblages are good indicators of environmental disturbance, such as coastal aquaculture, considering their rapid response in terms of diversity and abundance. The aim of this study was to evaluate the response of peracarid assemblages to the release of waste from coastal farming as these organisms play an important ecological role. Abundance and species richness did not show significant differences between farm and control localities but did show a high spatial variabili...

  2. Coordinated gene expression during gilthead sea bream skeletogenesis and its disruption by nutritional hypervitaminosis A

    OpenAIRE

    Zambonino-Infante Jose; Mazurais David; Andree Karl B; Darias Maria; Fernández Ignacio; Gisbert Enric

    2011-01-01

    Abstract Background Vitamin A (VA) has a key role in vertebrate morphogenesis, determining body patterning and growth through the control of cell proliferation and differentiation processes. VA regulates primary molecular pathways of those processes by the binding of its active metabolite (retinoic acid) to two types of specific nuclear receptors: retinoic acid receptors (RARs) and retinoid X receptors (RXRs), which promote transcription of downstream target genes. This process is well known ...

  3. Molecular Identification and Phylogenetic Relationships of Threadfin Breams (Family: Nemipteridae Using mtDNA Marker

    Directory of Open Access Journals (Sweden)

    Vaithilingam RAVITCHANDIRANE

    2012-05-01

    Full Text Available Cytochrome c oxidase-1 gene sequences of mitochondrial genome were analyzed for species identification and phylogenetic relationship among the commercially important Nemipterus species. Sequence analysis of COI gene clearly indicated that all the nine fish species fell into distinct clads, which are genetically distant from each other and exhibited identical phylogenetic reservation. All the COI gene sequences provide sufficient phylogenetic information and evolutionary relationship to distinguish the nine Nemipterus species unambiguously. As per the neighbour-joining (NJ and maximum likelihood (ML trees, all the nine species are genetically distant from each other and exhibited identical phylogenetic reservation. Based on the NJ and ML phylogenetic trees N. mesoprion, N. zysron, N. hexodon, N. nematophorus, N. virgatus and N. bipunctatus were closely related with high bootstrap value (97. The overall mean Kimura two parameter (K2P distances between the nine species was 0.109. The intra species K2P distance was high in N. japonicus (0.069 followed by N. peronii (0.050 and N. mesoprion (0.002. This study proves the use of mtDNA COI gene sequence based approach is an alternative tool for identifying fish species at a faster pace.

  4. Effects of sea bass and sea bream farming (Western Mediterranean Sea on peracarid crustacean assemblages

    Directory of Open Access Journals (Sweden)

    Fernandez–Gonzalez, V.

    2011-06-01

    Full Text Available Benthic soft-bottom assemblages are good indicators of environmental disturbance, such as coastal aquaculture, considering their rapid response in terms of diversity and abundance. The aim of this study was to evaluate the response of peracarid assemblages to the release of waste from coastal farming as these organisms play an important ecological role. Abundance and species richness did not show significant differences between farm and control localities but did show a high spatial variability at the two studied scales. Non-metric multi- dimensional scaling (MDS analysis showed a separation between farms and controls, indicating that peracarid assemblages are modified as a result of aquaculture activities, and some species such as Ampelisca spp. showed statistical differences. Peracarids, at both species and community level, may therefore be applied as helpful indicators to assess benthic effects of coastal farming.

  5. Shelf life determination of the brined golden mullet Liza aurata during vacuum refrigerated storage using some quality aspect

    Directory of Open Access Journals (Sweden)

    Mariyam Ali

    2012-03-01

    Full Text Available Background. Salted fish products are popular in many countries around the world. Salting is one of the oldest techniques for fish preservation, and is essentially intended to increase the shelf-life of the product depressing water activity by means of dehydration and salt uptake by the fish muscle. However, the current demand for salted fish is driven more by the flavour of the product than for preservation purposes. Vacuum-packaging represents a static form of hypobaric storage. It is widely used in the food industry because of its effectiveness in reducing oxidative reactions in the product at relatively low cost. Low temperature storage is one of the primary methods to maintain fish quality, based on the reduction in the rates of microbiological, chemical and biochemical changes. Material  and methods. Fresh Golden mullets were rapidly beheaded, scaled, gutted and immediately washed with tap water then, samples were taken to the laboratory in ice box for chemical and microbial analysis of fresh fish, other samples were put in the brine (6 liter water and 2160 g salt was used for brine solution. After 14 days of brining, fish were taken out of brine solution and drained, then they were Vacuum Packed and labelled (each pack contained two fish about 1500 g weight. All the packs were stored in a refrigerator 4°C. Some quality aspects including Total Volatile Nitrogen (TVN, Peroxide Value (PV, Thiobarbituric Acid (TBA, Total Viable Count (TVC, Halophilic Bacteria (HB and presence of Clostridium Botulinum were determined in fresh mullets, fresh brined mullets after 14 days of brining, and in (Vacuum Packed VP samples stored at 4°C at intervals of 30, 60 and 90 days. Results.  TVN increased from ten mg/100 g in fresh brined after 14 days to 30.80 mg/100 g in VP brined Golden mullet after 90 days of storage at 4°C, PV increased after brining from 1.50 meq/kg in fresh brined to 28.90 meq/kg in VP brined Golden mullet after 90 days of storage at 4°C, TBA increased from 0.07 mg MDA/kg in fresh brined to 0.10 after 60 days and then, decreased to 0.09 mg MDA/kg in VP brined Golden mullet after 90 days of storage and  TVC decreased from 4.70 log CFU/gr in fresh brined to 4.40 log CFU/ gr after 30 days and then, increased to 5.70 log CFU/gr in VP brined Golden mullet after 90 days of storage at 4°C, HB increased from 4.55 log CFU/gr in fresh brined to 6.30 log CFU/gr after 90 days of storage period at 4°C and exceeded the permissible level. Clostridium botulinum toxin was not detected in any of the samples throughout the storage. Conclusions. The results from this study clearly suggested that a combination of brining, vacuum packaging and storage at refrigerated temperature prolongs the shelf-life of Golden mullet to a great extent. Our findings revealed that the longest shelf-life was for VP brined Golden mullet stored at 4°C is 30 days.

  6. Shelf life determination of the brined golden mullet Liza aurata during vacuum refrigerated storage using some quality aspect

    OpenAIRE

    Mariyam Ali

    2012-01-01

    Background. Salted fish products are popular in many countries around the world. Salting is one of the oldest techniques for fish preservation, and is essentially intended to increase the shelf-life of the product depressing water activity by means of dehydration and salt uptake by the fish muscle. However, the current demand for salted fish is driven more by the flavour of the product than for preservation purposes. Vacuum-packaging represents a static form of hypobaric storage. It is widely...

  7. SPATIAL STRUCTURE OF HELMINTH COMMUNITIES IN THE GOLDEN GREY MULLET, LIZA AURATA (ACTINOPTERYGII: MUGILIDAE), FROM THE WESTERN MEDITERRANEAN

    Czech Academy of Sciences Publication Activity Database

    Míguez-Lozano, R.; Pardo-Carranza, T.V.; Blasco-Costa, Maria Isabel; Balbuena, J. A.

    2012-01-01

    Roč. 98, č. 5 (2012), s. 904-912. ISSN 0022-3395 Institutional support: RVO:60077344 Keywords : 1914 DIGENEA HAPLOPORIDAE * PARASITE SPECIES RICHNESS * N. SP MONOGENEA * MARINE FISHES * ENVIRONMENTAL COMPONENTS * LIGOPHORUS MONOGENEA * SATURNIUS MANTER * BETA DIVERSITY * DISTANCE DECAY * BLACK-SEA Subject RIV: EH - Ecology, Behaviour Impact factor: 1.321, year: 2012 http://www.bioone.org/doi/abs/10.1645/GE-2897.1#fn2

  8. Morphological recognition of artificial F1 hybrids between three common European cyprinid species: Rutilus rutilus, Blicca bjoerkna and Abramis brama.

    OpenAIRE

    Matondo, B.N.; Ovidio, Michaël; Poncin, Pascal; Vandewalle, Pierre; Philippart, Jean-Claude

    2008-01-01

    The F1 hybrids of roach Rutilus rutilus, silver bream Blicca bjoerkna, and common bream Abramis brama from experimental reciprocal cross-breedings were identified at 18 months of age in relation to the parental species. The morphological analysis, including quantitative (plastic and meristic) and nonmetric features differing in the roach, the silver bream and the common bream, revealed that roach * common bream and roach * silver bream hybrids were easier to distinguish from their parents tha...

  9. Vaccination strategies to prevent emerging diseases for Spanish aquaculture.

    Science.gov (United States)

    Romalde, J L; Ravelo, C; López-Romalde, S; Avendaño-Herrera, R; Magariños, B; Toranzo, A E

    2005-01-01

    In recent years, three serious diseases have emerged in Spanish aquaculture. These are lactococcosis caused by Lactococcus garvieae, which is of economical importance in rainbow trout (Oncorhynchus mykiss); pseudomonadiasis caused by Pseudomonas anguilliseptica which affects gilthead seabream (Sparus aurata) and turbot (Scophthalmus maximus); and flexibacteriosis caused by Tenacibaculum maritimum which became a devastating problem in the emerging culture of sole (Solea spp). To obtain useful information for the design and development of new vaccines, antigenic characterisation of representative strains was performed. In this work we present the strategies adopted for the vaccine formulation (strains included, use of adjuvants) and administration (route, necessity of booster, etc.). The results from laboratory and/or field vaccination trials performed showed that for lactococcosis, protection lasting for five months was obtained with an oil-adjuvanted bacterin formulation. Unadjuvanted bacterin gave only a short duration of protection, which could, however, be prolonged by an antigen boost administered via the feed. A bacterin against Pseudomonas anguilliseptica gave protection for 12 weeks when tested in an experimental challenge trial in turbot. Besides the flexibacteriosis vaccine developed by our group for turbot, and due to the antigenic host-associated variability within T. maritimum, a new bacterin was developed against this bacterium to be used specifically in sole. This new bacterin, administered to sole by intraperitoneal injection, yielded RPS values of 94 % six weeks after immunization. In conclusion, these results suggest that vaccination constitutes a cost-effective method of controlling diseases that have emerged in the most important fish species being cultured in Spain. PMID:15962472

  10. Using a concentrate of phenols obtained from olive vegetation water to preserve chilled food: two case studies

    Directory of Open Access Journals (Sweden)

    Luca Fasolato

    2016-05-01

    Full Text Available Phenols are plant metabolites characterised by several interesting bioactive properties such as antioxidant and bactericidal activities. In this study the application of a phenols concentrate (PC from olive vegetation water to two different fresh products – gilt-head seabream (Sparus aurata and chicken breast – was described. Products were treated in a bath of PC (22 g/L; chicken breast or sprayed with two different solutions (L1:0.75 and L2:1.5 mg/mL; seabream and then stored under refrigeration conditions. The shelf life was monitored through microbiological analyses – quality index method for seabream and a specific sensory index for raw breast. The secondary products of lipid-peroxidation of the chicken breast were determined using the thiobarbituric acid reactive substances (TBARs test on cooked samples. Multivariate statistical techniques were adopted to investigate the impact of phenols and microbiological data were fitted by DMfit software. In seabream, the levels of PC did not highlight any significant difference on microbiological and sensory features. DMfit models suggested an effect only on H2S producing bacteria with an increased lag phase compared to the control samples (C: 87 h vs L2: 136 h. The results on chicken breast showed that the PC bath clearly modified the growth of Pseudomonas and Enterobacteriaceae. The phenol dipping was effective in limiting lipid-peroxidation (TBARs after cooking. Treated samples disclosed an increase of shelf life of 2 days. These could be considered as preliminary findings suggesting the use of this concentrate as preservative in some fresh products.

  11. Post-treatment of biologically treated wastewater containing organic contaminants using a sequence of H2O2 based advanced oxidation processes: photolysis and catalytic wet oxidation.

    Science.gov (United States)

    Rueda-Márquez, J J; Sillanpää, M; Pocostales, P; Acevedo, A; Manzano, M A

    2015-03-15

    In this paper the feasibility of a multi-barrier treatment (MBT) for the regeneration of synthetic industrial wastewater (SIWW) was evaluated. Industrial pollutants (orange II, phenol, 4-chlorophenol and phenanthrene) were added to the effluent of municipal wastewater treatment plant. The proposed MBT begins with a microfiltration membrane pretreatment (MF), followed by hydrogen peroxide photolysis (H2O2/UVC) and finishing, as a polishing step, with catalytic wet peroxide oxidation (CWPO) using granular activated carbon (GAC) at ambient conditions. During the microfiltration step (0.7 μm) the decrease of suspended solids concentration, turbidity and Escherichia coli in treated water were 88, 94 and 99%, respectively. Also, the effluent's transmittance (254 nm) was increased by 14.7%. Removal of more than 99.9% of all added pollutants, mineralization of 63% of organic compounds and complete disinfection of total coliforms were reached during the H2O2/UVC treatment step (H2O2:TOC w/w ratio = 5 and an UVC average dose accumulated by wastewater 8.80 WUVC s cm(-2)). The power and efficiency of the lamp, the water transmittance and photoreactor geometry are taken into account and a new equation to estimate the accumulated dose in water is suggested. Remaining organic pollutants with a higher oxidation state of carbon atoms (+0.47) and toxic concentration of residual H2O2 were present in the effluent of the H2O2/UVC process. After 2.3 min of contact time with GAC at CWPO step, 90 and 100% of total organic carbon and residual H2O2 were removed, respectively. Also, the wastewater toxicity was studied using Vibrio fischeri and Sparus aurata larvae. The MBT operational and maintenance costs (O&M) was estimated to be 0.59 € m(-3). PMID:25600300

  12. Selective estrogen receptor modulators differentially alter the immune response of gilthead seabream juveniles.

    Science.gov (United States)

    Rodenas, M C; Cabas, I; García-Alcázar, A; Meseguer, J; Mulero, V; García-Ayala, A

    2016-05-01

    17α-ethynylestradiol (EE2), a synthetic estrogen used in oral contraceptives and hormone replacement therapy, tamoxifen (Tmx), a selective estrogen-receptor modulator used in hormone replacement therapy, and G1, a G protein-coupled estrogen receptor (GPER) selective agonist, differentially increased the hepatic vitellogenin (vtg) gene expression and altered the immune response in adult gilthead seabream (Sparus aurata L.) males. However, no information exists on the effects of these compounds on the immune response of juveniles. This study aims, for the first time, to investigate the effects of the dietary intake of EE2, Tmx or G1 on the immune response of gilthead seabream juveniles and the capacity of the immune system of the specimens to recover its functionality after ceasing exposures (recovery period). The specimens were immunized with hemocyanin in the presence of aluminium adjuvant 1 (group A) or 120 (group B) days after the treatments ceased (dpt). The results indicate that EE2 and Tmx, but not G1, differentially promoted a transient alteration in hepatic vtg gene expression. Although all three compounds did not affect the production of reactive oxygen intermediates, they inhibited the induction of interleukin-1β (il1b) gene expression after priming. Interestingly, although Tmx increased the percentage of IgM-positive cells in both head kidney and spleen during the recovery period, the antibody response of vaccinated fish varied depending on the compound used and when the immunization was administered. Taken together, our results suggest that these compounds differentially alter the capacity of fish to respond to infection during ontogeny and, more interestingly, that the adaptive immune response remained altered to an extent that depends on the compound. PMID:27012396

  13. Threats posed by artisanal fisheries to the reproduction of coastal fish species in a Mediterranean marine protected area

    Science.gov (United States)

    Lloret, J.; Muñoz, M.; Casadevall, M.

    2012-11-01

    Artisanal fisheries are frequently considered as a sustainable activity compatible with the conservation objectives of marine protected areas (MPAs). Few studies have examined the impacts of these fisheries on the reproductive potential of exploited fish species within the marine reserves. This study evaluated the potential impact of artisanal fishing on the reproduction of coastal fish species in a Mediterranean MPA through onboard sampling from January 2008 to December 2010. Eleven sex-changing fish species constituted an important part of the catch (20% overall and up to 60% of the total gill net catch) and, in five of them, most individuals were of one sex. Artisanal fishing can negatively affect the sustainability of those coastal fishes showing sex reversal, particularly the protogynous ones such as Diplodus cervinus and Epinephelus marginatus, as well as the species with complex mating systems (e.g. some sparids, labrids and scorpaenids). In all species the average size for the individuals captured was above the minimum landing size (where this exists), but in four species (Conger conger, Diplodus puntazzo, Sphyraena spp. and Sparus aurata) it was below the size of first maturity (L50). Results show that sex and size selection by artisanal fishing not only can have an impact on the reproduction of coastal fish species but may also be exacerbating rather than reducing the impact of fishing on coastal resources. Thus, new management actions need to be urgently implemented in the MPAs where artisanal fisheries are allowed to operate in order to protect the reproductive potential of these species, particularly those showing a complicated reproductive strategy.

  14. Sustainable Resource Use of Common Bream and Roach Catch from Reduction Fishing in Östergötland

    OpenAIRE

    Svensson, Malin

    2014-01-01

    Nutrient inflows from anthropogenic sources into water systems are causing eutrophication, algal blooms and trophic changes in Swedish lakes and seas. The European water framework directive was implemented to regulate member countries' policies to achieve a good status in surface waters. Reduction fishing has shown to be an effective lake restoration tool involving removal of large quantities of planktivorous fish, decreasing the internal nutrient loads and recovering the lake status. The Adm...

  15. Structure of spawning population of the bream Abramis brama in water bodies of the Dniprovsko-Orelsky nature reserve

    Directory of Open Access Journals (Sweden)

    D. L. Bondarev

    2006-12-01

    Full Text Available The population state of important food fish Abramis brama L. in the Dniprovsko-Orelsky Nature Reserve is under consideration . Basic population parameters of the species have been studied. Assessment of modern state and determination of perspectives of further existence of the roach in the Reserve’s basins have been done.

  16. Structure of spawning population of the bream Abramis brama in water bodies of the Dniprovsko-Orelsky nature reserve

    OpenAIRE

    D. L. Bondarev

    2006-01-01

    The population state of important food fish Abramis brama L. in the Dniprovsko-Orelsky Nature Reserve is under consideration . Basic population parameters of the species have been studied. Assessment of modern state and determination of perspectives of further existence of the roach in the Reserve’s basins have been done.

  17. Observation of reproductive behaviour of the common bream .i.Abramis Brama./i. by scientific echo sounder

    Czech Academy of Sciences Publication Activity Database

    Prchalová, M.; Kubečka, Jan

    Montpellier: ICES, 2002. s. 131-132. [6th ICES Symposium on Acoustics in Fisheries and Aquatic Ecology, Book of Abstracts.. 10.06.2002-14.06.2002, Montpellier] Institutional research plan: CEZ:AV0Z6017912 Keywords : spawning * echo sounder * Abramis Subject RIV: EH - Ecology, Behaviour

  18. Attachment ability of a clamp-bearing fish parasite, Diplozoon paradoxum (Monogenea), on gills of the common bream, Abramis brama.

    Science.gov (United States)

    Wong, Wey-Lim; Gorb, Stanislav N

    2013-08-15

    Monogeneans, which are mainly fish ectoparasites, use various types of haptoral (posterior) attachment apparatus to secure their attachment onto their hosts. However, it remains unclear how strongly a monogenean can attach onto its host. In the present study, we aimed for the first time to (1) measure pull-off forces required to detach a pair of clamp-bearing monogeneans, Diplozoon paradoxum, from gills of Abramis brama and (2) determine the contribution of muscles to the clamp movements. A mean force of 6.1±2.7 mN (~246 times the animals' weight) was required to dislodge a paired D. paradoxum vertically from the gills. There were significant differences (Pfiltered lake water (20.16±8.63 μm). This suggests that the closing of the clamps is probably not due to the continuous contraction of extrinsic muscles but is caused by the elasticity of the clamp material and that muscle activity is required for clamp opening. PMID:23580722

  19. Molecular cloning and expression of toll-like receptor 4 (tlr4) in the blunt snout bream (Megalobrama amblycephala).

    Science.gov (United States)

    Lai, Ruifang; Liu, Han; Jakovlić, Ivan; Zhan, Fanbin; Wei, Jin; Yang, Pinhong; Wang, Weimin

    2016-06-01

    Toll-like receptors (TLRs) play a pivotal role in teleost innate immune system. In this study, Megalobrama amblycephala (ma) tlr4 gene was cloned, its putative polypeptide product characterized, and expression analysed. Matlr4 cDNA is 2862 bp long, with an open reading frame of 2364 bp encoding 787 amino acids. MaTlr4 is a typical TLR protein, including the extracellular part with nine leucine-rich repeat motifs, a transmembrane region and a cytoplasmic Toll/interleukin-1 receptor domain. MaTlr4 has the highest level of identity (94%) and similarity (97%) with the grass carp Tlr4.2 homolog. This was also corroborated by the phylogenetic analysis, which placed MaTlr4 in a cluster with other cyprinid homologs. Matlr4 mRNA was ubiquitously expressed in all examined tissues and during all sampled developmental stages. The observed peak in matlr4 mRNA expression during gastrula and somite stages is in good agreement with its proposed role in the development of the neural system. Temporal expression patterns of matlr4 and maMyD88 mRNAs and proteins were analyzed in liver, spleen, head kidney, trunk kidney and intestine after Aeromonas hydrophila infection. And mRNA expression varied between different time-points. Both MaTlr4 and MaMyD88 protein expressions at 12 hpi were significantly enhanced in head kidney and intestine. These results indicate that matlr4 is involved in the immune response in M. amblycephala, and that it is indeed a functional homologue of tlr4s described in other animal species. PMID:26802439

  20. FEEDING HABITS OF THE SHARP SNOUT SEA BREAM, DIPLODUS PUNTAZZO (CETTI, 1777) (TELEOSTEI: SPARIDAE) FROM BENGHAZI COAST, EASTERN LIBYA

    OpenAIRE

    Hana M Saleh* and Mohammad El-Mor

    2015-01-01

    The feeding habits of 500 specimens of Diplodus puntazzo (family: Sparidae), inhabiting Benghazi Mediterranean coast, were studied monthly from September 2013 to August 2014. The annual diet composition, monthly variations in the diet composition, the variations of diet with length and the intensity of feeding were studied. Diplodus puntazzo feed on a wide variety of prey types: crustaceans) 40.1%), cephalopods (22.1%), green algae (17.7 %), mollusks (5.2%), polychaetes (1.1%) and sediments (...

  1. Molecular cloning, tissue expression of gene Muc2 in blunt snout bream Megalobrama amblycephala and regulation after re-feeding

    Science.gov (United States)

    Xue, Chunyu; Xi, Bingwen; Ren, Mingchun; Dong, Jingjing; Xie, Jun; Xu, Pao

    2015-03-01

    Mucins are important components of mucus, which form a natural, physical, biochemical and semipermeable mucosal layer on the epidermis of fish gills, skin, and the gastrointestinal tract. As the first step towards characterizing the function of Muc2, we cloned a partial Megalobrama amblycephala Muc2 cDNA of 2 175 bp, and analyzed its tissue-specific expression pattern by quantitative real-time PCR (qPCR). The obtained sequence comprised 41 bp 5'-untranslated region (5'-UTR), 2 134 bp open reading frame encoding a protein of 711 amino acids. BLAST searching and phylogenetic analysis showed that the predicted protein contained several common secreted mucin-module domains (VWD-C8-TIL-VWD-C8) and had high homology with mucins from other vertebrates. Among four candidate reference genes ( β- Actin, RPI13α, RPII, 18S) for the qPCR, RPII was chosen as an appropriate reference gene because of its lowest variation in different tissues. M. amblycephala Muc2 was mainly expressed in the intestine, in the order (highest to lowest) middle-intestine > fore-intestine > hind-intestine. Muc2 was expressed relatively poorly in other organs (brain, liver, kidney, spleen, skin and gill). Furthermore, after 20-days of starvation, M. amblycephala Muc2 expressions after refeeding for 0 h, 3 h, 16 h, 3 d, and 10 d were significantly decreased in the three intestinal segments ( P<0.05) at 16 h, and were then upregulated to near the initial level at 10 d.

  2. Isolation and confirmation of viral nervous necrosis (VNN) disease in golden grey mullet (Liza aurata) and leaping mullet (Liza saliens) in the Iranian waters of the Caspian Sea.

    Science.gov (United States)

    Zorriehzahra, M E J; Ghasemi, M; Ghiasi, M; Karsidani, S Haghighi; Bovo, G; Nazari, A; Adel, M; Arizza, V; Dhama, K

    2016-07-15

    The present study was conducted on 428 moribund mullet fish samples to isolate and identify the causative agent of a mysterious acute mortality which recently occurred in wild mullets in Iranian waters of Caspian Sea, suspected to be due to viral nervous necrosis (VNN) disease. Disease investigation was carried out employing various diagnostic procedures such as virology, bacteriology, parasitology, haematology, histopathology, IFAT, IHC and nested RT-PCR. Brain and eye samples of affected fishes were collected in sterile conditions and then kept at -80°C for cell culture isolation and nested RT-PCR detection of the causative agent. Other tissue samples were also collected and fixed for histopathology, IHC and EM examinations. CPE was observed in cell cultures at 6days after inoculation. Nine samples were found positive with virological assay. Nested RT-PCR, performed on suspected tissues and CPE positive samples, showed that about 21 tissue samples and all the CPE positive samples were positive for VNN virus (VNNV). IFAT was selected as a confirmatory method for detecting the presence of Betanodavirus antigen, cell culture isolation results and nested RT-PCR findings. Moreover, VNNV particles with 25-30nm in diameter were also visualized in the infected brain and retina. In pathogenicity studies, guppy fishes bathed in VNNV-infected tissue culture (10(-4) TCID50) showed clinical signs similar to naturally infected mullet after 15days post infection (dpi), with mortality rates reaching up to 100% at 30dpi. Affected organ samples as examined by cell culture isolation, IFAT, IHC and histopathology, revealed the presence of VNNV in the guppy fishes. In conclusion, it was confirmed that VNNV was the main causative agent for the disease outbreak in mullet fish in the Caspian Sea, and this is such first official report of VNN disease from Iran. PMID:27283853

  3. Contaminação por mercúrio e respostas de stresse oxidativo nos olhos de peixes selvagens (Liza aurata)

    OpenAIRE

    Pereira, Ricardo Jorge Silva

    2014-01-01

    Todos os animais usam os olhos para monitorizar o ambiente circundante e manter a homeostase. O Mercúrio (Hg) (incluindo o metilmercúrio - MeHg) é um contaminante pernicioso e omnipresente em águas naturais, mas a avaliação do seu potencial para interferir com olhos de peixe é um problema maioritariamente inexplorado. Esta tese irá contribuir para preencher a lacuna de conhecimento correspondente à avaliação da acumulação de mercúrio na parede do olho de tainha-garrento (Liz...

  4. Nodavirus Colonizes and Replicates in the Testis of Gilthead Seabream and European Sea Bass Modulating Its Immune and Reproductive Functions

    Science.gov (United States)

    Valero, Yulema; Arizcun, Marta; Esteban, M. Ángeles; Bandín, Isabel; Olveira, José G.; Patel, Sonal; Cuesta, Alberto; Chaves-Pozo, Elena

    2015-01-01

    Viruses are threatening pathogens for fish aquaculture. Some of them are transmitted through gonad fluids or gametes as occurs with nervous necrosis virus (NNV). In order to be transmitted through the gonad, the virus should colonize and replicate inside some cell types of this tissue and avoid the subsequent immune response locally. However, whether NNV colonizes the gonad, the cell types that are infected, and how the immune response in the gonad is regulated has never been studied. We have demonstrated for the first time the presence and localization of NNV into the testis after an experimental infection in the European sea bass (Dicentrarchus labrax), and in the gilthead seabream (Sparus aurata), a very susceptible and an asymptomatic host fish species, respectively. Thus, we localized in the testis viral RNA in both species using in situ PCR and viral proteins in gilthead seabream by immunohistochemistry, suggesting that males might also transmit the virus. In addition, we were able to isolate infective particles from the testis of both species demonstrating that NNV colonizes and replicates into the testis of both species. Blood contamination of the tissues sampled was discarded by completely fish bleeding, furthermore the in situ PCR and immunocytochemistry techniques never showed staining in blood vessels or cells. Moreover, we also determined how the immune and reproductive functions are affected comparing the effects in the testis with those found in the brain, the main target tissue of the virus. Interestingly, NNV triggered the immune response in the European sea bass but not in the gilthead seabream testis. Regarding reproductive functions, NNV infection alters 17β-estradiol and 11-ketotestosterone production and the potential sensitivity of brain and testis to these hormones, whereas there is no disruption of testicular functions according to several reproductive parameters. Moreover, we have also studied the NNV infection of the testis in vitro to

  5. SPECIFIC FEATURES OF DISTRIBUTING AND ACCUMULATION Of HEAVY METALS ARE IN ORGANISMS Of THE FISHES BENTOPHAGES Of KREMENCHUG STORAGE POOL

    Directory of Open Access Journals (Sweden)

    А. Melnik

    2013-03-01

    Full Text Available Maintenance of heavy metals is investigational in organs and fabrics of the fishes bentophages - bream (Abramis brama L., roach (Rutilus rutilus L. and white bream (Blicca bjoerkna L. of the Kremenchug storage pool. It is set that after maintenance of heavy metals in organs and fabrics in the direction of increase of their concentration it is possible to present such row: bream < white bream < roach.

  6. SPECIFIC FEATURES OF DISTRIBUTING AND ACCUMULATION Of HEAVY METALS ARE IN ORGANISMS Of THE FISHES BENTOPHAGES Of KREMENCHUG STORAGE POOL

    OpenAIRE

    А. Melnik; N. Vlasova; E. Kolos; A. Didenko

    2013-01-01

    Maintenance of heavy metals is investigational in organs and fabrics of the fishes bentophages - bream (Abramis brama L.), roach (Rutilus rutilus L.) and white bream (Blicca bjoerkna L.) of the Kremenchug storage pool. It is set that after maintenance of heavy metals in organs and fabrics in the direction of increase of their concentration it is possible to present such row: bream < white bream < roach.

  7. In situ diel patterns of zooplankton consumption by subadult/adult roach, Rutilus rutilus, bream, Abramis brama, and bleak Alburnus alburnus

    Czech Academy of Sciences Publication Activity Database

    Vašek, Mojmír; Kubečka, Jan

    2004-01-01

    Roč. 53, č. 2 (2004), s. 203-214. ISSN 0139-7893 R&D Projects: GA AV ČR(CZ) IAA6017201 Keywords : feeding periodicity * gut fullness * cyprinids Subject RIV: EG - Zoology Impact factor: 0.536, year: 2004

  8. Sexual ornamentation and parasite infection in males of common bream (Abramis brama): a reflection of immunocompetence status or simple cost of reproduction?

    Czech Academy of Sciences Publication Activity Database

    Ottová, E.; Šimková, A.; Jurajda, Pavel; Dávidová, M.; Ondračková, Markéta; Pečínková, M.; Gelnar, M.

    2005-01-01

    Roč. 7, č. 4 (2005), s. 581-593. ISSN 1522-0613 R&D Projects: GA ČR(CZ) GA524/04/1128; GA ČR(CZ) GP524/03/P108 Institutional research plan: CEZ:AV0Z60930519 Keywords : cost of reproduction * immunocompetence * sexual ornamentation Subject RIV: EG - Zoology Impact factor: 1.614, year: 2005

  9. Growth and development of vimba bream (Vimba vimba) larvae in relation to feeding duration with live and/or dry starter feed

    Czech Academy of Sciences Publication Activity Database

    Hamáčková, J.; Prokeš, Miroslav; Kozák, P.; Peňáz, Milan; Stanny, L. A.; Policar, T.; Baruš, Vlastimil

    2009-01-01

    Roč. 287, 1-2 (2009), s. 158-162. ISSN 0044-8486 R&D Projects: GA MZe QH71305 Institutional research plan: CEZ:AV0Z60930519 Keywords : ASTA starter feed * Artemia salina * Total length * Weight * Level of ontogeny Subject RIV: GL - Fishing Impact factor: 1.925, year: 2009

  10. Effects of Ascophyllum and Spirulina Meal as Feed Additives on Growth Performance and Feed Utilization of Red Sea Bream, Pagrus major

    OpenAIRE

    Mustafa, Md. Ghulam; Takeda, Taka-aki; UMINO, Tetsuya; Wakamatsu, Shigeru; Nakagawa, Heisuke

    1994-01-01

    養魚飼料への藻類添加は生理状態の向上に有効であることが明らかにされているので、本研究ではマダイについて Ascophyllum と Spirulina の効果をみた。対照区には配含飼料に5%のセルロース、Ascophyllum 区、Spirulina 区にはそれぞれ藻類の粉末を5%添加した。初期体重85gのマダイ1年魚を77日間飼育し、成長、飼料利用率、生物学的性状、体成分に及ぼす効果をみた。いずれの藻類の添加においても成長、飼料効率、タンパク質効率、筋肉タンパク質蓄積量が向上し、筋肉成分では脂質蓄積量に増加がみられた。Spirulina の投与では悪影響は認められず、各項目においてAscophyllum より優れた効果を示し、これまでに報告された藻類に匹敵するか、もしくはそれ以上の効果を示した。...

  11. De novo assembly of the blunt snout bream (Megalobrama amblycephala) gill transcriptome to identify ammonia exposure associated microRNAs and their targets.

    Science.gov (United States)

    Sun, Shengming; Ge, Xianping; Zhu, Jian; Zhang, Wuxiao; Xuan, Fujun

    2016-01-01

    De novo transcriptome sequencing is a robust method for microRNA (miRNA) target gene prediction, especially for organisms without reference genomes. Following exposure of Megalobrama amblycephala to ammonia (0.1 or 20 mg L(-1) ), two cDNA libraries were constructed from the fish gills and sequenced using Illumina HiSeq 2000. Over 90 million reads were generated and de novo assembled into 46, 615 unigenes, which were then extensively annotated by comparing to different protein databases, followed by biochemical pathway prediction. The expression of 2666 unigenes significantly differed; 1961 were up-regulated, while 975 were down-regulated. Among these, 250 unigenes were identified as the targets for 10 conserved and 4 putative novel miRNA families by miRNA target computational prediction. We examined expression of ssa-miRNA-21 and its target genes by real-time quantitative PCR and found agreement with the sequencing data. This study demonstrates the feasibility of identifying miRNA targets by transcriptome analysis. The transcriptome assembly data represent a substantial increase in the genomic resources available for Megalobrama amblycephala and will be useful for gene expression profile analysis and miRNA functional annotation. PMID:27504260

  12. Cultivation of gilthead bream in monoculture and integrated multi-trophic aquaculture. Analysis of production and environmental effects by means of the FARM model

    DEFF Research Database (Denmark)

    Ferreira, J.G.; Saurel, Camille; Ferreira, J.M.

    Abstract: The aquaculture growth required to meet increasing protein demand by a growing world population, predicted to reach 9billion people by 2050, is driving innovation in both siting and culture practice. Limited possibilities for expansion on land and in inshore coastal areas, and technolog...... quality obtained from wild fish, and above that limit there is a rapid increase of the feed conversion ratio (FCR) and cultivation becomes financially unattractive....

  13. The use of multivariate analysis to link sediment contamination and toxicity data to establish sediment quality guidelines: an example in the Gulf of Cadiz (Spain); El uso del analisis multivariante en la union de datos de toxicidad y contaminacion para establecer guias de calidad de sedimento: Un ejemplo en la Bahia de Cadiz (Espana)

    Energy Technology Data Exchange (ETDEWEB)

    Del Valls, T. Angel; Forja, Jesus M [Departamento de Quimica Fisica, Facultad de Ciencias del Mar, Universidad de Cadiz, Cadiz, (Spain); Gomez-Parra, Abelardo [Instituto de Ciencias Marinas de Andalucia, CSIC, Puerto Real, Cadiz, (Spain)

    1998-03-01

    To evaluate marine sediment quality in the Gulf of Cadiz, chemical concentration and toxicity test endpoints from sediments were linked using multivariate analysis. Sediment samples were collected synoptically at seven stations in two littoral ecosystems of the Gulf of Cadiz (five in the Bay of Cadiz and two in the salt marsh of the Barbate River), and subjected to six separate, replicated sediment toxicity tests and comprehensive sediment chemistry analyses. The toxic effects of sediments were tested using three operational sediment phases: whole sediment, using the estuarine amphipod Microdeutopus gryllotalpa (10 d static: survival) and the estuarine clam Ruditapes philippinaru (48 h static: survival) and of the marine fish Sparus aurata (48 h static. Survival); and interstitial water, using populations of the estuarine rotifer Brachionus Plicatilis (7 d static: Population decline) and of the marine bacteria Photobacterium phosphoreum (Microtox ). To evaluate the levels of contamination, the concentrations in the sediments of organic carbon, 14 trace metals (Fe, Mn, Cu, Zn, Pb, Cd, Ag, Hg, As, Sn, V, Ni, Co and Cr) and the surfactant linear alkylbenzenesulphonate (LAS) were measured. The results of the toxicity tests were compared in a dose-response relationship between sites, demonstrating a general agreement between the toxicity values determined by all the tests, except in the case of interstitial water toxicity (principally due to toxic mixtures of trace metals). Data derived from sediment chemistry and bioassays were assembled by multivariate statistical techniques (PCA and factor analysis), showing that the two data types could be represented by only five factors corresponding to five overlapping chemical-biological effect relations. Positive prevalence of these factors in the cases studied was used to establish those ranges in chemical concentrations associated with adverse effects. The sediment quality guidelines, in terms of concentrations at or below

  14. Гистопатологии некоторых внутренних органов у леща (abramis brama linnaeus, 1758) Саратовского водохранилища

    OpenAIRE

    Минеев, А. К.

    2014-01-01

    The estimation of the absolute number of bream in the Volga-Caspian and North Caspian fishery subareas is given. The modern mathematical method of cohort analysis and the direct method used during the researches give a complete picture of the bream population and allow determining its reserves and the total allowable catch for the future under different environmental conditions and rational use of spawning stock. The reasons of changing the current dynamics of bream population such as changin...

  15. Strategies to enhance the competitiveness of semi-intensive aquaculture systems in costal earth ponds: the organic aquaculture approach

    Directory of Open Access Journals (Sweden)

    Manuel Sardinha

    2014-06-01

    Full Text Available Earthen ponds are the main production system for European seabass (Dicentrarchus labrax and gilthead seabream (Sparus aurata in Portugal and in Southern Spain. Production costs in this low productivity farming system are higher compared to intensive cage farms, and its economic sustainability depends on product differentiation and optimization of production. The development of new farming protocols, to enhance productivity of ponds and lagoons, or the implementation of certification processes to add value to products from extensive and semi-intensive systems, could render these activities more economics benefits and be attractive for a new generation of farmers. One clear strategy to differentiate and add value to seabream produced in semi-intensive systems is its production under organic standards. Feeds often represent 40-60% of the operational costs of fish farming. In relation to the traditional feeds, the organic certified feeds currently present a price-differential of about +30%. To assess it economic feasibility, it is therefore essential to validate if such higher feeding costs will represent an increase on productivity (fish growth and feed conversion or can be fully integrated in the final sale price. Throughout a full production cycle of gilthead seabream (from 9 grams until >350 grams, a series of trials allowed us to assess the effects of: a an organic certified feed; b variable feeding levels (100 and 80% of recommendations; and c culture density (0.5 and 1.0 kg/m3 on the overall growth performance, body composition and environmental impact at the fish farm level. Results show that growth performance (weight gain and feed conversion ratio of seabream fed the organic feed was similar to that found in fish fed a traditional diet. With the organic feed, a reduction of the feeding level by 20% of the manufacturer recommendations led to a lower growth, while a similar reduction on the feeding level of the traditional feed had no impact

  16. 腌制和干燥工艺对咸金线鱼中生物胺的影响%Effect of picking and drying process on biogenic amines formation in salted threadfin bream (Nemipterus hexodon)

    Institute of Scientific and Technical Information of China (English)

    陈玉峰; 吴燕燕; 邓建朝; 黄卉; 李来好; 杨贤庆; 戚勃; 周婉君

    2015-01-01

    为探明腌制和干燥工艺对咸鱼产品中生物胺的影响,本文以金线鱼(Nemipterus hexodon)为原料,通过柱前衍生HPLC技术分析金线鱼在不同腌制条件和不同干燥方式下,鱼体中8种生物胺及其相关指标的变化.结果表明:食盐对鱼体中生物胺生成有明显抑制作用(p<0.05),25%食盐腌制的金线鱼总胺量为(50.82±7.18) mg/kg,分别比食盐添加量为0%、8%和15%的总胺量降低了89.7%、45.9%和40.1%;生物胺及其相关指标的含量均随腌制温度的升高、腌制时间的延长而明显增加,0℃腌制时的总胺量为(76.72±0.69) mg/kg比15℃和25℃的总胺量分别降低9.5%、26.1%,15℃腌制时,腌制第4d比第1d总胺量增加58.9%;组胺是金线鱼腌制过程最优势的生物胺;干燥方式显著影响咸金线鱼生物胺含量变化,其中日晒方式产品的总胺量为(430.13±3.48) mg/kg,与真空干燥(60 ℃,-0.1~0.09 MPa)(404.61±9.23) mg/kg相近,30℃冷风干燥较热风干燥有利于抑制咸鱼生物胺的产生,总胺量、生物胺指数(BAI)和质量指数(QI)均最低,最能保证产品的品质和安全性.

  17. An annotated list of fish parasites (Isopoda, Copepoda, Monogenea, Digenea, Cestoda, Nematoda) collected from Snappers and Bream (Lutjanidae, Nemipteridae, Caesionidae) in New Caledonia confirms high parasite biodiversity on coral reef fish

    Czech Academy of Sciences Publication Activity Database

    Justine, J.-L.; Beveridge, I.; Boxshall, G.A.; Bray, R. A.; Miller, T.L.; Moravec, František; Trilles, J.-P.; Whittington, I.D.

    2012-01-01

    Roč. 8, č. 1 (2012), s. 22. ISSN 2046-9063 R&D Projects: GA ČR GBP505/12/G112 Institutional support: RVO:60077344 Keywords : biodiversity * coral reef s * parasites * coextinction * New Caledonia * South Pacific Subject RIV: EG - Zoology

  18. An annotated list of fish parasites (Copepoda, Monogenea, Digenea, Cestoda and Nematoda) collected from Emperors and Emperor Bream (Lethrinidae) in New Caledonia further highlights parasite biodiversity estimates on coral reef fish

    Czech Academy of Sciences Publication Activity Database

    Justine, J.-L.; Beveridge, I.; Boxshall, G.A.; Bray, R. A.; Moravec, František; Whittington, I.D.

    2010-01-01

    Roč. 2691, - (2010), s. 1-40. ISSN 1175-5326 R&D Projects: GA MŠk LC522 Institutional research plan: CEZ:AV0Z60220518 Keywords : fish * new host records * new geographical records * inventory * biogeography * South Pacific Subject RIV: GJ - Animal Vermins ; Diseases, Veterinary Medicine Impact factor: 0.853, year: 2010

  19. Struktura zooplanktonu v potravě cejna velkého (Abramis brama) v průběhu biomanipulace

    OpenAIRE

    Zapletal, T.

    2014-01-01

    The structure of zooplankton in diet of common bream (Abramis brama) was analysed in the Hamry water supply reservoir during the period 2011-2014. Fish were captured by gill netting, beach seining and electrofishing in the littoral part of reservoir. Minimum twenty specimens of each size group were taken for analysis on each sampling occasion. Zooplankton in the diet of adult bream SL 210-315 mm and subadult SL 124-186 mm made recedent proportion. Juvenile bream (0+) SL 18-51 mm consumed zoop...

  20. Formation of a local Abramis brama orientalis (BERG) population in the zone of hot effluent from the Konakovo hydroelectric power plant

    Energy Technology Data Exchange (ETDEWEB)

    Sappo, G.B.

    1976-01-01

    Bream (Abramis brama orientalis) inhabitating the part of the Ivan'kovo Reservoir directly exposed to effluent from the Konakovo Hydroelectric Power Plant are smaller and become sexually mature sooner than elsewhere in the reservoir. Moreover, they have longer tail peduncles and anal, thoracic, and dorsal fins than other bream. And, in contrast with the latter, many (20 percent) have two rows of pharyngeal teeth. Thus, the differences in ecological (growth rate, condition factor, spawning times, etc.) and meristic and plastic characters (branched rays, enlarged fins, etc.) as well as in composition of parasites point to the development of an isolated bream population.

  1. Severe fish mortality associated with 'red tide' observed in the sea off Cochin

    Digital Repository Service at National Institute of Oceanography (India)

    Naqvi, S.W.A; George, M.D.; Narvekar, P.V.; Jayakumar, D.A; Shailaja, M.S.; Sardessai, S.; Sarma, V.V.S.S.; Shenoy, D.M.; Naik, H.; Maheswaran, P.A; KrishnaKumari, L.; Rajesh, G.; Sudhir, A; Binu, M.S.

    Severe fish mortality associated with the "red tide" phenomenon caused by Noctiluca blooms was observed in the sea off Cochin, Kerala, India at depths less than 40 m. The dead fish, almost entirely comprised of the threadfin bream (Nemipterus...

  2. Cholesterol content in meat of some Cyprinidae

    Directory of Open Access Journals (Sweden)

    Živković Dragić L.

    2002-01-01

    Full Text Available The aim of this paper was to examine cholesterol content in meat of five Cyprinidae species: white bream (Bllica bjoerkna L, carp bream (Abramis brama L, baltic vimba (Vimba vimba carinata Pallas, zope (Abramis balerus L and crucian carp (Carassius carassius gibelio Bloch from the river Danube. Cholesterol content was examined in the function of season factor and individual weight. Cholesterol concentration in meat of white bream carp bream, baltic vimba, zope and crucian carp is on average level below 20 mg/100 g of meat, which makes meat of these fish species nutritively very valuable. Cholesterol content is variable during the season. Its concentration in meat and in lipids is lowest during spring, during summer it increases and during autumn decreases, except in meat of white bream. Body weight has influence on cholesterol content when its concentration is expressed as % of cholesterol in lipids. Its content in lipids decreases with increasing of individual weight, except in meat of carp bream.

  3. Is humane slaughter of fish possible for industry?

    NARCIS (Netherlands)

    Vis, van de J.W.; Kestin, S.; Robb, D.; Oehlenschlaeger, J.; Lambooij, E.; Kuhlmann, H.; Kloosterboer, K.

    2003-01-01

    The objective was to evaluate industrial and research slaughter methods for Atlantic salmon (Salmo salar), gilt-head seabream (Sparus auratus) and eel (Anguilla anguilla) with respect to welfare and quality. As a general term of reference, an optimal slaughter method should render fish unconscious u

  4. Retrospective monitoring of mercury in fish from selected European freshwater and estuary sites.

    Science.gov (United States)

    Nguetseng, Regine; Fliedner, Annette; Knopf, Burkhard; Lebreton, Benoit; Quack, Markus; Rüdel, Heinz

    2015-09-01

    Levels and trends of total mercury (THg) and methylmercury (MeHg) compounds in bream (Abramis brama) from different European sites were compared. Bream were collected between 2007 and 2013 in the estuaries of the rivers Scheldt (Netherlands), Rhône (France), Göta älv (Sweden), Tees (United Kingdom), and Mersey (UK), and in Lake Belau (Germany). A direct mercury analyzer was used to determine THg concentrations while MeHg was measured by gas chromatography/inductively coupled plasma-mass spectrometry applying stable isotope dilution. THg and MeHg in annual pool samples of bream ranged between 15.9 and 251 μg kg(-1) wet weight (ww) with lowest concentrations found at the reference site Lake Belau and highest in samples from the river Rhône. The EU environmental quality standard (EQS) of 20 μg kg(-1) ww was exceeded at all sites and in all years except at Lake Belau in 2012. Significantly decreasing trends over time were observed only in bream from the Rhône, while THg increased in bream from the Western Scheldt. The MeHg fractions of THg were always >80% and a significant difference between sites was detected only in one case (Rhône vs. Lake Belau). PMID:25989521

  5. Hybridisation between two cyprinid fishes in a novel habitat: genetics, morphology and life-history traits

    Directory of Open Access Journals (Sweden)

    Caffrey Joe

    2010-06-01

    Full Text Available Abstract Background The potential role hybridisation in adaptive radiation and the evolution of new lineages has received much recent attention. Hybridisation between roach (Rutilus rutilus L. and bream (Abramis brama L. is well documented throughout Europe, however hybrids in Ireland occur at an unprecedented frequency, often exceeding that of both parental species. Utilising an integrated approach, which incorporates geometric morphometrics, life history and molecular genetic analyses we identify the levels and processes of hybridisation present, while also determining the direction of hybridisation, through the analysis of mitochondrial DNA. Results The presence of F2 hybrids was found to be unlikely from the studied populations, although significant levels of backcrossing, involving both parental taxa was observed in some lakes. Hybridisation represents a viable conduit for introgression of genes between roach and bream. The vast majority of hybrids in all populations studied exhibited bream mitochondrial DNA, indicating that bream are maternal in the majority of crosses. Conclusions The success of roach × bream hybrids in Ireland is not due to a successful self reproducing lineage. The potential causes of widespread hybridisation between both species, along with the considerations regarding the role of hybridisation in evolution and conservation, are also discussed.

  6. A dot immunobinding assay (DIA) to detect the exotoxin produced by the pathogenic bacteria of the vibriosis in marine cage-cultured Pagrus major%海水养殖真鲷弧菌病病原菌外毒素的点酶法检测

    Institute of Scientific and Technical Information of China (English)

    吴后波; 潘金培

    2003-01-01

    Vibriosis, caused by Vibrio mimicus, became a major cause of serious economic losses in the farming of red sea bream along the coast of southern China. It has been demonstrated that the pathogenicity of V. mimicus for red sea bream is due to the production of a heat-labile exotoxin. In the present paper, a dot immunobinding assay (DIA) to detect the toxin produced by V. mimicus was developed. The lowest concentration of the toxin the DIA could detect was 0. 025/μg·mL-1 The DIA could detect the toxin in the supernatant of liver and spleen of the diseased red sea bream Pagrus major, even if the supernatant was diluted by 1000 times. It was concluded that the DIA would be useful for rapid and effective detection of the toxin in large samples.

  7. The anti-inflammatory drugs diclofenac, naproxen and ibuprofen are found in the bile of wild fish caught downstream of a wastewater treatment plant.

    Science.gov (United States)

    Brozinski, Jenny-Maria; Lahti, Marja; Meierjohann, Axel; Oikari, Aimo; Kronberg, Leif

    2013-01-01

    Pharmaceutical residues are ubiquitous in rivers, lakes, and at coastal waters affected by discharges from municipal wastewater treatment plants. In this study, the presence of 17 different pharmaceuticals and six different phase I metabolites was determined in the bile of two wild fish species, bream (Abramis brama) and roach (Rutilus rutilus). The fish were caught from a lake that receives treated municipal wastewater via a small river. Prior to analyses, the bile content was enzymatically hydrolyzed to convert the glucuronide metabolites into the original pharmaceuticals or phase I metabolites. The solid phase extracts of hydrolyzates were analyzed by liquid chromatography-tandem mass spectrometry (LC-MS/MS) in the multiple reaction monitoring mode. The anti-inflammatory drug naproxen could be detected in all the six bream and roach bile samples. Diclofenac was found in five of the bream and roach samples, while ibuprofen was detected in three bream and two roach samples. The observed bile concentrations of diclofenac, naproxen, and ibuprofen in bream ranged from 6 to 95 ng mL(-1), 6 to 32 ng mL(-1), and 16 to 34 ng mL(-1), respectively. The corresponding values in roach samples ranged from 44 to 148 ng mL(-1), 11 to 103 ng mL(-1) and 15 to 26 ng mL(-1), respectively. None of the other studied compounds could be detected. The study shows that pharmaceuticals originating from wastewater treatment plant effluents can be traced to the bile of wild bream and roach living in a lake where diclofenac, naproxen, and ibuprofen are present as pollutants. PMID:23186122

  8. 温度对平鲷胚胎发育的影响

    Institute of Scientific and Technical Information of China (English)

    李加儿; 丁彦文; 周宏团; 马荣和

    1987-01-01

    In this paper, the influence of temperature on the embryonic developments of flat-bream (Rhabdosargus sarba) is reported.The parent fish of the flatbream cultured in the pond were induced by using HCG and LRH-A and the fertilized eggs produced through artificial propagation were reared under five different water temperatures. The result of the experiment showed that the embryos of flat-bream were relatively sensitive to the change of the temperature, and its optimal temperature for the development was 21-4℃.

  9. PIXE analysis of otoliths from several species of teleost fish

    International Nuclear Information System (INIS)

    In-air PIXE was used to analyze trace elements in otoliths from several species of teleost fish in order to examine the correlation between the trace elements concentrations and environmental conditions. Mn, Fe, Zn and Sr were detected accurately in the order of ppm by using the in-air PIXE. It seemed that concentrations of Sr and Zn in red sea bream otoliths increased in proportion to higher seawater temperature. In addition, there were significant differences in trace element composition between that of reared red sea bream and rockfish and that of wild ones. Preliminary results indicate that the PIXE is a powerful technique to investigate fish otoliths. (author)

  10. PIXE analysis of otoliths from several species of teleost fish

    Energy Technology Data Exchange (ETDEWEB)

    Arai, Nobuaki; Sakamoto, Wataru [Kyoto Univ. (Japan). Faculty of Agriculture; Maeda, Kuniko

    1994-12-31

    In-air PIXE was used to analyze trace elements in otoliths from several species of teleost fish in order to examine the correlation between the trace elements concentrations and environmental conditions. Mn, Fe, Zn and Sr were detected accurately in the order of ppm by using the in-air PIXE. It seemed that concentrations of Sr and Zn in red sea bream otoliths increased in proportion to higher seawater temperature. In addition, there were significant differences in trace element composition between that of reared red sea bream and rockfish and that of wild ones. Preliminary results indicate that the PIXE is a powerful technique to investigate fish otoliths. (author).

  11. Dicty_cDB: Contig-U11679-1 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available Contig-U11679-1 gap included 1377 2 6572587 6573856 PLUS 1 2 U11679 0 0 0 0 0 0 0 0 0 1 0 0 0 0 ... known mRNA. 48 0.90 1 ( DQ095195 ) Profelis aurata sex -determining region Y protein ... 48 0.90 1 ( DQ095 ... 194 ) Oncifelis geoffroyi sex -determining region Y prot... 48 0.90 1 ( DQ095192 ...

  12. Dispersant use as a response to oil spills: toxicological effects on fish cardiac performance

    OpenAIRE

    Milinkovitch, Thomas; Thomas-Guyon, Hélène; Lefrançois, Christel; Imbert, Nathalie

    2013-01-01

    Dispersant use is a controversial technique used to respond to oil spills in nearshore areas. In order to assess the toxicity of this technique, this study evaluated the cardiac toxicological effects on juvenile golden grey mullets Liza aurata exposed for 48 h to either dispersant alone, chemically dispersed oil, mechanically dispersed oil, the water soluble fraction of oil or to a control condition. Following exposure, the positive inotropic effects of adrenaline were assessed in order to ev...

  13. Assessment of heavy metal concentrations in water, plankton, and fish of Lake Manzala, Egypt

    OpenAIRE

    BAHNASAWY, Mohamed; KHIDR, Abdel-Aziz; DHEINA, Nadia

    2011-01-01

    The levels of some heavy metals (Cu, Zn, Cd, and Pb) were determined in water, plankton, and fish (Liza aurata) collected from 5 sites in Lake Manzala. Metals in the water and fish exhibited a significant seasonal and regional variation, in which all metals attained their maximum value during summer, while the lowest levels were found during winter. The concentration of different metals in water, plankton, and fish tissues followed the same order: Zn > Cu > Pb > Cd. The mean concentr...

  14. Humane killing of fishes for scientific research: a comparison of two methods.

    Science.gov (United States)

    Blessing, J J; Marshall, J C; Balcombe, S R

    2010-06-01

    Two killing methods were compared on the clupeid, bony bream Nematolosa erebi and it was found that ice-slurry immersion was more humane than benzocaine overdose. The use of ice-slurry for killing N. erebi should be accepted as a standard humane method and considered similarly for other warm-water species. PMID:20557609

  15. Short-term and long-term effects of zooplanktivorous fish removal in a shallow lake: a synthesis of 15 years of data from Lake Zwemlust

    NARCIS (Netherlands)

    Van de Bund, W.J.; Van Donk, E.

    2002-01-01

    1. Removal of zooplanktivorous fish (mainly bream) in 1987 from a shallow eutrophic lake in the Netherlands, Lake Zwemlust, resulted in a quick switch from a turbid state with cyanobacteria blooms to a clear state dominated by macrophytes. 2. The clear state was not stable in the long term, however,

  16. Ectoparasites increase swimming costs in a coral reef fish

    OpenAIRE

    Binning, Sandra A.; Roche, Dominique G.; Layton, Cayne

    2013-01-01

    Ectoparasites can reduce individual fitness by negatively affecting behavioural, morphological and physiological traits. In fishes, there are potential costs if ectoparasites decrease streamlining, thereby directly compromising swimming performance. Few studies have examined the effects of ectoparasites on fish swimming performance and none distinguish between energetic costs imposed by changes in streamlining and effects on host physiology. The bridled monocle bream (Scolopsis bilineatus) is...

  17. Biomass and abundance biases in European standard gillnet sampling.

    Science.gov (United States)

    Šmejkal, Marek; Ricard, Daniel; Prchalová, Marie; Říha, Milan; Muška, Milan; Blabolil, Petr; Čech, Martin; Vašek, Mojmír; Jůza, Tomáš; Monteoliva Herreras, Agustín; Encina, Lourdes; Peterka, Jiří; Kubečka, Jan

    2015-01-01

    The European Standard EN 14757 recommends gillnet mesh sizes that range from 5 to 55mm (knot-to-knot) for the standard monitoring of fish assemblages and suggests adding gillnets with larger mesh sizes if necessary. Our research showed that the recommended range of mesh sizes did not provide a representative picture of fish sizes for larger species that commonly occur in continental Europe. We developed a novel, large mesh gillnet which consists of mesh sizes 70, 90, 110 and 135mm (knot to knot, 10m panels) and assessed its added value for monitoring purposes. From selectivity curves obtained by sampling with single mesh size gillnets (11 mesh sizes 6 - 55mm) and large mesh gillnets, we identified the threshold length of bream (Abramis brama) above which this widespread large species was underestimated by European standard gillnet catches. We tested the European Standard gillnet by comparing its size composition with that obtained during concurrent pelagic trawling and purse seining in a cyprinid-dominated reservoir and found that the European Standard underestimated fish larger than 292mm by 26 times. The inclusion of large mesh gillnets in the sampling design removed this underestimation. We analysed the length-age relationship of bream in the Římov Reservoir, and concluded that catches of bream larger than 292mm and older than five years were seriously underrepresented in European Standard gillnet catches. The Římov Reservoir is a typical cyprinid-dominated water body where the biomass of bream > 292mm formed 70% of the pelagic trawl and purse seine catch. The species-specific relationships between the large mesh gillnet catch and European Standard catch suggested that the presence of carp (Cyprinus carpio), European catfish (Silurus glanis), tench (Tinca tinca) or bream warrants the use of both gillnet types. We suggest extending the gillnet series in the European Standard to avoid misinterpretation of fish community biomass estimates. PMID:25793776

  18. Influence of pH upon the activity of glycosidases and proteinases of intestinal mucosa, chyme and microbiota in fish.

    Science.gov (United States)

    Kuz'mina, V V; Skvortsova, E G; Zolotareva, G V; Sheptitskiy, V A

    2011-09-01

    It is shown that amylolytic and proteolytic activity of the intestinal mucosa, the chyme and the intestinal flora in the fishes, zander Zander lucioperca (L.), perch Perca fluviatilis L., bream Abramis brama (L.) and roach Rutilus rutilus (L.), belonging according to their feeding habits to different ecological groups at the same pH values as well as in the pH range from 5.0 to 10.0 considerably varies. The glycosidase pH optimum of the mucosa and intestinal microbiota is 7.0, whereas that of the chyme varies from 6.0 (in roach) to 8.0 (in bream). pH optimum of the mucosa proteinases in all fish species is 10.0, whereas that of the chyme and the bacterial flora can be observed in all the range of pH values. PMID:21082240

  19. Physiological and ecological factors influencing the radiocaesium contamination of fish species from Kiev reservoir

    International Nuclear Information System (INIS)

    The monitoring of 137Cs contamination of fish from the northern part of the Kiev reservoir between 1987 and 1992 indicated systematic differences in the long-term average contamination levels of different species, which are closely related to their trophic levels. The average contamination of the predatory species: perch (Perca fluviatilis) and pike (Esox lucius) were roughly factors of 6.3 and 4.4 respectively, higher than those of the nonpredatory species: bream (Abramis brama), silver bream (Blicca bjoerkna) and rudd (Scardinius erythrophothalmus). For tench (Tinca tinca) and goldfish (Carassius sp.), this factor is 2. The solution of the caesium balance equation in fish obtained for equilibrium conditions, which is applicable to the long-term contamination, provides some explanations for these accumulation differences between the species with respect to influences by different ecological and physiological factors

  20. Physiological and ecological factors influencing the radiocaesium contamination of fish species from Kiev reservoir

    Energy Technology Data Exchange (ETDEWEB)

    Koulikov, A.O. [A.N. Severtzov Institute of Evolutionary Morphology and Ecology of Animals, Russian Academy of Sciences, Moscow (Russian Federation)

    1995-11-23

    The monitoring of {sup 137}Cs contamination of fish from the northern part of the Kiev reservoir between 1987 and 1992 indicated systematic differences in the long-term average contamination levels of different species, which are closely related to their trophic levels. The average contamination of the predatory species: perch (Perca fluviatilis) and pike (Esox lucius) were roughly factors of 6.3 and 4.4 respectively, higher than those of the nonpredatory species: bream (Abramis brama), silver bream (Blicca bjoerkna) and rudd (Scardinius erythrophothalmus). For tench (Tinca tinca) and goldfish (Carassius sp.), this factor is 2. The solution of the caesium balance equation in fish obtained for equilibrium conditions, which is applicable to the long-term contamination, provides some explanations for these accumulation differences between the species with respect to influences by different ecological and physiological factors.

  1. Host specificity, pathogenicity, and mixed infections of trypanoplasms from freshwater fishes.

    Science.gov (United States)

    Losev, Alexander; Grybchuk-Ieremenko, Anastasiia; Kostygov, Alexei Yu; Lukeš, Julius; Yurchenko, Vyacheslav

    2015-03-01

    This work summarizes the results of the 8-year study focused on Trypanoplasma sp. parasitizing freshwater fishes in the vicinity of Kyiv, Ukraine. Out of 570 fish specimens of 2 different species analyzed, 440 individuals were found to be infected. The prevalence of infection ranged from 24 % in Abramis brama Linnaeus (freshwater bream) to 100 % in Cobitis taenia Linnaeus (spined loach). The level of parasitemia also varied between moderate in freshwater bream and very high in spined loach. Interestingly, no clinical manifestations of trypanoplasmosis were observed even in extremely heavily infected C. taenia. We hypothesize that different species may differ in evolutionary timing allowing for reciprocal adaptation of the members of the "host-parasite" system. Molecular analysis of the 18S rRNA sequences revealed that several specimens were simultaneously infected with at least two different trypanoplasm species. To the best of our knowledge, this is the first report of the mixed infection with fish trypanoplasms. PMID:25544706

  2. Antibacterial and immunity enhancement properties of anaesthetic doses of thyme (Thymus vulgaris oil and three other anaesthetics in Sparidentax hasta and Acanthopagrus latus

    Directory of Open Access Journals (Sweden)

    I.S. Azad

    2014-04-01

    Full Text Available An effective alternative was discovered in the form of thyme oil for use as a fish anaesthetic (patent pending approval. The thyme oil along with a common aquaculture-grade commercial anaesthetic (AQUI-S, clove oil and quinaldine were investigated for their antimicrobial properties and its effect on the immune parameters of two important maricultured fish species, bluefin bream (Sparidentax hasta and yellowfin bream (Acanthopagrus latus. In vivo studies indicated that both the fish species had highly reduced bacterial load after the treatments and the in vitro antibacterial activity of the of the thyme oil was superior to that of the other treatments. The effects of anaesthetic dose of thyme oil, clove oil, quinaldine and AQUI-S were evaluated and compared. The reduction in the total viable vibrio counts in the anesthetized fish indicated that the vibrio were sensitive to the thyme oil. Also thyme oil produced higher non-specific immune enhancements.

  3. Cormorant predation on PIT-tagged lake fish

    DEFF Research Database (Denmark)

    Skov, Christian; Jepsen, Niels; Baktoft, Henrik;

    2014-01-01

    The present study use data from recovered PIT (Passive Integrated Transponder) tags to explore species-and size-specific annual predation rates by cormorants on three common lacustrine fishes (size range 120-367 mm) in a European lake; roach (Rutilus rutilus), common bream (Abramis brama) and perch...... (Perca fluviatilis). In addition, we quantify the level of age/size truncation that cormorant predation could introduce in a population of perch, an important fish for recreational angling as well as for trophic interactions and ecosystem function in European lakes. Based on three years of PIT tagging of...... fish in Lake Viborg and subsequent recoveries of PIT tags from nearby cormorant roosting and breeding sites, we show that cormorants are major predators of roach, bream and perch within the size groups we investigated and for all species larger individuals had higher predation rates. Perch appear to be...

  4. Studies on fish scale collagen of Pacific saury (Cololabis saira).

    Science.gov (United States)

    Mori, Hideki; Tone, Yurie; Shimizu, Kouske; Zikihara, Kazunori; Tokutomi, Satoru; Ida, Tomoaki; Ihara, Hideshi; Hara, Masayuki

    2013-01-01

    We purified and characterized Type I collagen from the scales of the Pacific saury (Cololabis saira) and compared it with collagen from other organisms. Subunit composition of C. saira collagen (2α1+α2) was similar to that of red sea bream (Pagrus major) and porcine collagen. C. saira collagen did not form a firm gel after neutralization of pH in solution. The temperature of denaturation (24-25 °C) of C. saira collagen was slightly lower than that of P. major collagen (26-27 °C). The contents of proline and hydroxyproline were lower in red sea bream and Pacific saury collagen than in porcine collagen. Circular dichroism spectra and Fourier-transformed infrared spectra showed that heat denaturation caused unfolding of the triple helices in all three collagens. PMID:25428059

  5. Biosynthetic Gene Cluster of Cetoniacytone A, an Unusual Aminocyclitol from the Endosymbiotic Bacterium Actinomyces sp. Lu 9419

    OpenAIRE

    Wu, Xiumei; Flatt, Patricia M.; Xu, Hui; Mahmud, Taifo

    2009-01-01

    A gene cluster responsible for the biosynthesis of the antitumor agent cetoniacytone A was identified in Actinomyces sp. strain Lu 9419, an endosymbiotic bacteria isolated from the intestines of the rose chafer beetle (Cetonia aurata). The nucleotide sequence analysis of the 46 kb DNA region revealed the presence of 31 complete ORFs, including genes predicted to encode a 2-epi-5-epi-valiolone synthase (CetA), a glyoxalase/bleomycin resistance protein (CetB), an acyltransferase (CetD), an FAD-...

  6. The Prevalence and Pathogenicity of the “Anchor Worm” (Lernea spp, Phylum Arthropoda) within the Finfish Inhabiting the Danube Delta Area

    OpenAIRE

    Marius Hangan; Laura Daniela Urdes; Daniela Ianitchi

    2013-01-01

    This research was conducted as a series of cross-sectional epidemiological studies. It started in the 2003 and ended in 2008, having as sample sites the Sontea-Fortuna, Gorgova-Uzlina, Dunavat-Dranov and Razim-Sinoie lakes. The aim of the research was to assess the distribution and the pathogenicity of the Lernaea copepod among the inhabiting finfish populations, in various seasons of the time period. Lernaea spp was found in ctenopharyngodon idella, cyprinus carpio, liza aurata and liza haem...

  7. Příjem potravy cejnem velkým (Abramis brama) v období tření

    OpenAIRE

    Zeman, Jan

    2011-01-01

    The main objective of this thesis was to assess food intake by bream, depending on the onset and end of spawning period. To meet the objectives of a practical experiment was carried out. This experiment was run on the dams Hamry and Brno in the years 2008 and 2009. Subsequently it was determined by standard methods. These methods (indirect, food frequency, filling index, index of superiority and gonadosomatic index. Subsequently, an analysis of stomach.

  8. Hodnocení kontaminace ryb ve významných rybářských revírech

    OpenAIRE

    ČERVENÝ, Daniel

    2012-01-01

    The object of this thesis is the assessment of fish contamination by selected foreign substances as toxic metals, polychlorinated biphenyls and pesticides in 31 selected fishing grounds. The monitoring was realized between years 2006 and 2010. Fish were caught by electric device and fishing rods. Concentrations of Hg, Cd, Pb, PCB, DDT, HCH and HCB in fish muscle tissues were monitored. Common bream (Abramis brama) was selected as a reference species for a comparison of the monitored sites. Th...

  9. Struktura zooplanktonu v potravě cejna velkého (Abramis brama) v průběhu biomanipulace

    Czech Academy of Sciences Publication Activity Database

    Zapletal, T.; Špaček, J.; Jurajda, Pavel; Všetičková, Lucie

    Brno: Mendelova univerzita, 2014 - (Kopp, R.), s. 226-231 ISBN 978-80-7509-153-6. [65 let výuky rybářství na Mendelově univerzitě v Brně. Brno (CZ), 02.12.2014-03.12.2014] R&D Projects: GA ČR GBP505/12/G112 Institutional support: RVO:68081766 Keywords : common bream * biomanipulation Subject RIV: GL - Fishing

  10. Biodiversity, Bioindication and Helminth communities of Abramis brama (Linnaeus, 1758) from the Danube River and Lake Srebarna, Bulgaria

    OpenAIRE

    KIRIN, Diana; HANZELOVÁ, Vladimira; SHUKEROVA, Sonya; KUZMANOVA, Dimitrinka

    2014-01-01

    Biomonitoring from water of the River Danube and Srebarna Lake was performed using freshwater fish and their parasites and parasite communities as bioindicators. During 2013, 78 specimens of freshwater bream (Abramis brama (L., 1758) were examined with standard techniques for parasites. Three species of parasites (Gyrodactylus elegans Nordmann, 1832, Diplozoon paradoxum Nordmann, 1832 and Pomphorhynchus tereticollis (Rudilphi, 1809)) were fixed. The analysis of the dominant structure of the f...

  11. Existují pohlavní rozdíly v žaberních aparátech našich kaprovitých ryb?

    OpenAIRE

    Bartoň, Daniel

    2015-01-01

    The main objective of presented review is to describe filter-feeding adjustments in common Czech cyprinid fish (common bream Abramis brama, roach Rutilus rutilus and bleak Alburnus alburnus) in the context of inter-sex differences. Branchial sieve used for filtering small particles is formed by branchial arches and connected branchial rakers. Shape, structure, size and number of branchial rakers are crucial for efficiency of filter-feeding. These parameters differ between species as well as b...

  12. Fish community and fisheries management of Brno Reservoir following revitalisation measures

    OpenAIRE

    Jurajda, P.; Z. Adámek; Valová, Z. (Zdenka); Janáč, M. (Michal); Roche, K.

    2015-01-01

    Brno Reservoir (259 ha) is one of the most intensively utilised waterbodies in the Czech Republic, being used simultaneously for recreation, sport, fisheries, boating and electric power production. Despite this, no consistent fish survey has yet been performed at the reservoir. Between 2009 and 2012, a number of measures, including fish biomanipulation, were applied to improve water quality. Large cyprinids (mainly common bream Abramis brama) were removed and predatory fish (e.g. pike Esox lu...

  13. Possible applications of modern fish larviculture technology to ornamental fish production

    OpenAIRE

    Dhert, P.; Lim, L C; Candreva, P.; Van Duffel, H; Sorgeloos, P.

    1997-01-01

    There has been rapid development in the marine foodfish larviculture technology in Europe since the early eighties, especially in the flat fish, turbot and halibut, and the bass and bream species. The most significant improvements in the eighties were the introduction of light control, artificial reproduction techniques, appropriate water treatment and the use of rotifers and Artemia nauplii of specific sizes and in the late eighties and early nineties the quality enhancement of live food org...

  14. 続・魚類養殖業--ブリ類およびマダイの経営分析

    OpenAIRE

    小野, 征一郎

    2013-01-01

    The Report of the Statistical Survey on Fishery Management, published by the Ministry of Agriculture, Forestry and Fisheries, was used to analyze the business performance of the fish aquaculture industry, specifically the yellowtail and red sea bream aquaculture industries, from 2005 to 2011. Focusing on corporate businesses, the core economic entities of the industry, profitability analysis, break-even point analysis (the ultimate index), and solvency analysis were performed. In addition, pr...

  15. Commercial and game fish from the Dąbie lake (Poland and their infection of Paracoenogonimus ovatus (Digenea

    Directory of Open Access Journals (Sweden)

    Angelika Anna Linowska

    2015-11-01

    Full Text Available Paracoenogonimus ovatus is the parasite that infest wide definitive host range (predator birds and at least two intermediate hosts: molluscs (Viviparus viviparus, V. fasciatus and freshwater fish from eastern and central Europe. The larvae of the parasite (metacercariae go to the muscle and connective tissue, and at high intensity of infection to the internal organs and brain of fish. A total of 128 fishes were caught in autumn 2009 and 2014, by fishing boats operating in Lake Dąbie, which is one of the largest lakes in Poland. The species selected reflect the species structure of commercial and recreational catches. They were roach, bream, silver bream, crucian carp (Cyprinidae, ruffe, perch, zander (Percidae and pike (Esocidae. The fish were filleted, and the skinned muscle was compressed under a trichinoscope, what allowed to see and count metacercariae in the tissues. To facilitate parasite isolation from the cysts, the muscle tissues were subjected to a 0.1 % solution of activated pepsin and 5 % citric acid at a temperature of 20ºC. The free larvae were measured and identified under an Olympus BX 50 microscope coupled with a camera running AxioVs40 V 4.8.2.0 software. Metacercariae are grouped primarily at depths ranging from just beneath the skin to about 50% of fillet thickness. The infection prevalence of P. ovatus metacercariae in the fish analyzed was 78,5%. The density of infection per gram of muscle tissue mass in the fish infected was 8.85 (from 1 to 84 metacercariae. The highest density was noted in roach, bream and white bream (17.09, 11.14 and 9.58, respectively. Metacercariae occurred singly in pike, parasites were not found in the muscles of crucian carp, ruffe, and perch examined. The reason for such differences in infection is the availability and composition of food, as well as the behavior of the fish examined.

  16. DMPD: Signaling by IL-12 and IL-23 and the immunoregulatory roles of STAT4. [Dynamic Macrophage Pathway CSML Database

    Lifescience Database Archive (English)

    Full Text Available 15546391 Signaling by IL-12 and IL-23 and the immunoregulatory roles of STAT4. Watf...ord WT, Hissong BD, Bream JH, Kanno Y, Muul L, O'Shea JJ. Immunol Rev. 2004 Dec;202:139-56. (.png) (.svg) (.html) (.csml) Show Signal...ing by IL-12 and IL-23 and the immunoregulatory roles of STAT4. PubmedID 15546391 Title Signal

  17. Is PCBs concentration variability between and within freshwater fish species explained by their contamination pathways?

    OpenAIRE

    Lopes, C; Perga, M.E.; Peretti, A; Roger, M.C.; Persat, H.; Babut, M.

    2011-01-01

    Many chemical, physiological, and trophic factors are known to affect ioaccumulation of polychlorinated biphenyls (PCBs) in biota. Understanding the primary factors affecting fish contamination is critical for predicting and assessing risks to upper-trophic level consumers, including humans. Here we identify PCB contamination pathways that could explain within- and between-species variability in fish concentration levels. Three freshwater river fish species (barbel, chub and bream) were sampl...

  18. Anreicherung von chlororganischen Verbindungen und Nitromoschusduftstoffen in der aquatischen Nahrungskette

    OpenAIRE

    Willitzkat, Fred R.

    2010-01-01

    Abstract Accumulation of organochlorines and nitro-musks in the aquatic food chain Organochlorines (OC) and nitro-musks were analysed in sediment and fish (homogenized whole-fish samples) from the lake Hohner See in central Schleswig-Holstein and in fish otters (Lutra lutra L.), which originated from the whole state. The fish belonged to different trophic levels: Esox lucius (pike), Perca fluviatilis (perch), Abramis brama (bream), Gymnocephalus carnuus (ruffe) and Scardinius er...

  19. Progress in live food production and use in fish and shellfish hatcheries

    OpenAIRE

    Sorgeloos, P.

    1998-01-01

    Different species of microalgae, the rotifer, Brachionus spp., and the brine shrimp, Artemia spp., are essential live food in the farming of several species of molluscs (e.g. oyster, clam, scallop), crustaceans (e.g. marine shrimp, freshwater prawn, crabs) and fish (e.g. bass, bream, grouper, turbot, flounder, halibut, ayu). A brief overview is given of the latest developments in their production and use in industrial hatcheries.

  20. Interactive Drivers of Activity in a Free-Ranging Estuarine Predator

    OpenAIRE

    Taylor, Matthew D.; McPhan, Luke; van der Meulen, Dylan E.; Gray, Charles A.; Payne, Nicholas L.

    2013-01-01

    Animal activity patterns evolve as an optimal balance between energy use, energy acquisition, and predation risk, so understanding how animals partition activity relative to extrinsic environmental fluctuations is central to understanding their ecology, biology and physiology. Here we use accelerometry to examine the degree to which activity patterns of an estuarine teleost predator are driven by a series of rhythmic and arrhythmic environmental fluctuations. We implanted free-ranging bream A...

  1. ASTAXANTHIN: A POTENTIAL CAROTENOID

    OpenAIRE

    Jyotika Dhankhar et al.

    2012-01-01

    Astaxanthin, a member of the carotenoid family, is a dark-red pigment which is the main carotenoid found in the marine world of algae and aquatic animals. Astaxanthin, is present in many types of seafood, including salmon, trout, red sea bream, shrimp and lobster, as well as in birds such as flamingo and quail. Synthetic Astaxanthin dominates the world market but recent interest in natural sources of the pigment has increased substantially. Common sources of natural Astaxanthin, are the gree...

  2. Aprovechamiento de recursos pesqueros infrautilizados para la obtención de alimentos mejorados para el cultivo de peces

    OpenAIRE

    Aurrekoetxea, G.; Perera, M.N.

    2002-01-01

    Several underutilised fishery resources were analysed, including low market value species and fish processing byproducts, and then one was selected due to its composition, amount and availability for high quality protein production for juvenile gilthead sea bream and turbot diets. Among the available extraction technologies, enzymatic hydrolysis was selected, since the resulting final product has high nutritional value. Four commercial enzymes were tested to determine their activity, and the ...

  3. CHARACTERISTICS AND NEW DATA OF RIVER ILOVA ICHTHYOFAUNA (CENTRAL CROATIA)

    OpenAIRE

    Dušan Jelić; Petar Žutinić; Mišel Jelić

    2009-01-01

    During the research 28 species of freshwater fish were found, belonging to 10 families. Most numerous family was Cyprinidae (18 species), followed by Percidae (3 species) and Cobitidae (2 species). Results showed that the most abundant in species were spirlin (Alburnoides bipunctatus) (17.4%), common roach (Rutilus rutilus) (15.7%) and prussian carp (Carassius gibelio) (12.8%). And in the terms of total fish weight, highest represented were asp (Aspius aspius) (28.9%), bream (Abramis brama) (...

  4. A notable Ichthyological find in the Kruger National Park

    Directory of Open Access Journals (Sweden)

    U de V. Pienaar

    1971-05-01

    Full Text Available A notable ichthyological find has been made in the Kruger National Park with the recording, during April 1970, of a specimen of the marine fish, Acanthopagrus berda Forskal near the confluence of the Crocodile and Komati Rivers. This is the first record of the River or Mud Bream within the fresh water river system of the Transvaal and also the furthest point inland that this fish has been collected.

  5. The Distribution of Posthodiplostomum Cuticola and Rossicotrema Donicum in Scardinius Erythrophthalmus and Hypophtalmichthys Molitrix Stemming from the Danube Delta

    OpenAIRE

    Cristiana Diaconescu; Laura Urdeş; Ştefan Diaconescu; Marius Hangan

    2010-01-01

    We investigated the pathogenicity of Posthodiplostomum cuticola and Rossicotrema donicum digenean infestations within freshwater fish populations, in the Gorgova – Uzlina, Dunavat – Dranov and Razim – Sinoie natural complexes. The so-called “Black spot disease” caused by the metacercarial infestation was diagnosed in 5 out of 11 studied freshwater fish species: rudd (Scardinius erythrophthalmus), silver carp (Hypophtalmichthys molitrix), zarte (Vimba vimba), bream (Abramis brama) and European...

  6. Fish otoliths analysis by PIXE: application to stock discrimination

    International Nuclear Information System (INIS)

    Fish otoliths are continuously deposited from fish birth to its death along with encoding environmental information. In order to decode the information, PIXE was adopted as trace elemental analysis of the otoliths. Strontium to calcium concentration ratios of red sea bream otoliths varied among rearing stations. The Sr/Ca ratios of Lake Biwa catfishes also varied between male and female and among fishing grounds. The PIXE analysis was applied to the fish stock discrimination. (author)

  7. Fish otoliths analysis by PIXE: application to stock discrimination

    Energy Technology Data Exchange (ETDEWEB)

    Arai, Nobuaki; Takai, Noriyuki; Sakamoto, Wataru [Kyoto Univ. (Japan). Faculty of Agriculture; Yoshida, Koji; Maeda, Kuniko

    1996-12-31

    Fish otoliths are continuously deposited from fish birth to its death along with encoding environmental information. In order to decode the information, PIXE was adopted as trace elemental analysis of the otoliths. Strontium to calcium concentration ratios of red sea bream otoliths varied among rearing stations. The Sr/Ca ratios of Lake Biwa catfishes also varied between male and female and among fishing grounds. The PIXE analysis was applied to the fish stock discrimination. (author)

  8. ELECTRON MICROSCOPE EVIDENCE OF VIRUS INFECTION IN CULTURED MARINE FISH

    Institute of Scientific and Technical Information of China (English)

    2000-01-01

    Electron microscope investigation on the red sea bream (Pagrosomus major), bastard halibut (Paralichthys olivaceus) and stone flounder (Kareius bicoloratus) in North China revealed virus infection in the bodies of the dead and diseased fish. These viruses included the lymphocystis disease virus (LDV), parvovirus, globular virus, and a kind of baculavirus which was not discovered and reported before and is now tentatively named baculavirus of stone flounder (Kareius bicoloratus).

  9. 養殖生簀内における魚群遊泳音の特徴

    OpenAIRE

    藤枝, 繁; 松野, 保久; 山中, 有一; 鄭, 龍晋; 岸本, 力; フジエダ, シゲル; マツノ, ヤスヒサ; ヤマナカ, ユウイチ; キシモト, チカラ; Fujieda, Shigeru; MATSUNO, Yasuhisa; Yamanaka, Yuichi; CHUNG, Yong-jin; KISHIMOTO, Chikara

    1993-01-01

    This paper describes some feature of the swimming sounds for fishes within the netting cages were recorded at Hayato culture ground in the innermost area of Kagoshima Bay. Subjects of investigation were Yellowtail Seriola quinqueradiata, Amberjack Seriola dumerili, Amberjack Seriola lalandi, Red Sea Bream Pagrus major, and Tiger Puffer Takifugu rubripes. The recorded underwater sounds were analyzed with the three kinds of method in order to obtain the characteristics of wave forms and frequen...

  10. Diminished Metal Accumulation in Riverine Fishes Exposed to Acid Mine Drainage over Five Decades

    OpenAIRE

    Ross A Jeffree; Markich, Scott J.; Twining, John R.

    2014-01-01

    Bony bream (Nematalosa erebi) and black catfish (Neosilurus ater) were sampled from the fresh surface waters of the Finniss River in tropical northern Australia, along a metal pollution gradient draining the Rum Jungle copper/uranium mine, a contaminant source for over five decades. Paradoxically, populations of both fish species exposed to the highest concentrations of mine-related metals (cobalt, copper, lead, manganese, nickel, uranium and zinc) in surface water and sediment had the lowest...

  11. Contents of radionuclides and heavy metals in fish roe of commercial fish of the Zaporizhya reservoir

    International Nuclear Information System (INIS)

    Comprehensive radiation-toxicological studies of fish roe of some commercial fish of the Zaporizhya Reservoir were conducted. It was found that the greatest number of 137Cs (4,5 Bq/kg) and 90Sr (3,2 Bq/kg) was accumulated by fish roe of perch. Heavy metals (cadmium, copper, zinc and lead), and natural radionuclides 226Ra and 232Th were accumulated to a greater amount of bream fish roe

  12. Persistent organic pollutants in muscle of fish collected from the Nové Mlýny reservoir in Southern Moravia, Czech Republic.

    Science.gov (United States)

    Zelníčková, Lenka; Svobodová, Zdeňka; Maršálek, Petr; Dobšíková, Radka

    2015-07-01

    The aim of the present study was to investigate the content of organochlorine pesticides (OCPs) and polychlorinated biphenyls (PCBs) in muscle tissues of fish, i.e. carp (Cyprinus carpio, L.), bream (Abramis brama, L.) and pike perch (Stizostedion lucioperca, L.) from the middle Nové Mlýny reservoir and compare our results with previous corresponding studies. Samples were analysed by gas chromatography with ion trap tandem mass spectrometry. The highest contents of all pollutants were determined in muscle tissue of bream. The analysis of HCHs showed that β-HCH was the most abundant. PCB congener 28 was evaluated as the predominant PCB congener. The metabolite p,p'-DDE exhibited the highest concentration of all the monitored metabolites. The monitoring confirmed significantly (P < 0.01) higher concentrations of OCP and PCB in muscle of bream compared to carp and pike perch. The contents of pollutants in our study were found to be lower in comparison to the findings of some previous studies. PMID:26092236

  13. Trophic interaction between topmouth gudgeon (Pseudorasbora parva and the co-occurring species during summer in the Dniprodzerzhynsk reservoir

    Directory of Open Access Journals (Sweden)

    Didenko A.V.

    2015-01-01

    Full Text Available Topmouth gudgeon, Pseudorasbora parva is the most common invasive fish in Europe, which can compete with the juveniles of valuable commercial fishes. The goal of this work is to study the diet of topmouth gudgeon and trophic relationships with some native fishes inhabiting the littoral zone of the Dniprodzerzhynsk reservoir. The obtained relatively low values of diet overlaps between topmouth gudgeon and other co-occurring cyprinids such as juvenile roach, Rutilus rutilus; silver bream, Blicca bjoerkna; rudd, Scardinius erythrophthalmus; Prussian carp, Carassius gibelio and bleak, Abramis brama, indicate that it is not a serious food competitor for them, especially commercially valuable ones. On the contrary, high diet overlaps were observed among some native cyprinids such as juvenile roach, silver bream, rudd, and bleak. The major prey items of topmouth gudgeon were Chydorus sphaericus and chironomids, whereas other cyprinids selected Bosmina spp. This peculiarity may be due to different vertical distributions of these fish in the littoral zone of the reservoir, where topmouth gudgeon inhabit near-bottom water layers, where they preyed on near-bottom and benthic zooplankters and chironomids, while juvenile roach, silver bream, rudd, and juvenile and adult bleak live in higher water layers, where they preyed on pelagic zooplankton.

  14. Comparative karyological analysis of four diplozoid species (Monogenea, Diplozoidae), gill parasites of cyprinid fishes.

    Science.gov (United States)

    Košková, Eva; Spakulová, Marta; Koubková, Božena; Reblánová, Marianna; Orosová, Martina

    2011-04-01

    The present study has revealed new data on chromosome complements of diplozoid parasites, namely Diplozoon paradoxum from freshwater bream Abramis brama, Paradiplozoon bliccae from white bream Blicca bjoerkna, Paradiplozoon sapae from white-eye bream Ballerus sapa, and Paradiplozoon nagibinae from zope Ballerus ballerus. Particularly, D. paradoxum is characterized by four pairs (2n=8) of long (up to 22.1 μm) chromosomes: pairs 1, 2, and 3 are metacentric and pair 4 acrocentric. Karyotypes of three Paradiplozoon species are nearly identical in number and morphological classification of chromosomes, each comprising diploid number of 14 one-armed chromosomes of very similar length ranging up to 12.5 μm in P. bliccae, 9.2 μm in P. sapae, and 9.9 μm in P. nagibinae. All four species are similar in their total complement length, ranging from 64.4 to 50.4 μm. Interspecific differences were found in location of secondary constriction: it is situated on short arm of the 1st chromosome pair in D. paradoxum, on long arm of the 7th pair in P. bliccae and on long arm of the 4th pairs in P. sapae and P. nagibinae. Phylogenetic interrelationship within the diplozoids and hypothetic karyotype evolution is here discussed. PMID:20981442

  15. Fish fauna of the lower reaches of the River Drava and surrounding marshland habitats near Donji Miholjac (Eastern Croatia

    Directory of Open Access Journals (Sweden)

    Marko Ćaleta

    2012-12-01

    Full Text Available A field research of ichthyofauna of the lower reaches of the River Drava near Donji Miholjac was made during 2006 using fishing nets and electrofisher. Additional data was collected from the local anglers to present all caught species in this area. Sampling was conducted on all types of water habitats including side arms, artificial channels, backwater arms and the main river channel. A total of 44 fish species were reported. Six species were documented from the catch of local anglers. The family Cyprinidae is represented by 24 species, Percidae by 4, Cobitidae by 3, Gobiidae and Centrarchidae by 2, while the remaining families were represented by 1 species. The most abundant species in this part of the River Drava is roach (Rutilus rutilus. The largest part of total biomass belongs to bream (Abramis brama. Other important species according to abundance in the examined area are: bitterling (Rhodeus amarus, European perch (Perca fluviatilis, silver bream (Blicca bjoerkna and common bleak (Alburnus alburnus. According to the ichthyofauna composition, the explored area is classified as a typical bream zone which is characteristic for the lower part of the river.

  16. Who Is Who: An Anomalous Predator-Prey Role Exchange between Cyprinids and Perch.

    Science.gov (United States)

    Vejřík, Lukáš; Matějíčková, Ivana; Seďa, Jaromír; Blabolil, Petr; Jůza, Tomáš; Vašek, Mojmír; Ricard, Daniel; Matěna, Josef; Frouzová, Jaroslava; Kubečka, Jan; Říha, Milan; Čech, Martin

    2016-01-01

    Piscivory in cyprinids (Cyprinidae) is extremely rare. Specifically, common bream (Abramis brama) and common carp (Cyprinus carpio) are zooplanktivorous fish in deep lentic waters. Nevertheless, we observed predation by these two cyprinids under natural conditions in the Vír Reservoir, Czech Republic. We conducted diet analysis for cyprinids caught by trawling and gillnets and the large amount of young-of-the-year (YOY) perch (Perca fluviatilis), with sizes of 37-52 mm standard length, were found in their digestive tracts. In 2010, a large amount of YOY perch caused a significant decrease in Daphnia spp. size and abundance in the reservoir. Hence, a food deficit was induced for the cyprinids, apparent also from the poor nutritional condition of common bream which was much worse than the condition of those in similar reservoirs. Common carp and common bream shifted to forced piscivory, and they utilized the YOY perch as an alternative food source. In contrast, smaller species, such as roach (Rutilus rutilus) and bleak (Alburnus alburnus), widely utilized planktonic cyanobacteria. In the following year, YOY perch occurred in significantly lower numbers and conversely, Daphnia spp. size and abundance were significantly higher. The forced piscivory was not observed. Our results indicate a switch to forced piscivory by cyprinids, which was caused by a shortage of their natural food source. Moreover, this phenomenon presents an effective mechanism for reduction in the numbers of YOY perch, ensuring the stability of the ecosystem. PMID:27276078

  17. Morphology of underyearling fishes in the zone affected by warm waters discharged from the Konakovo Power Station into Ivan'kovskoye reservoir

    Energy Technology Data Exchange (ETDEWEB)

    Boytsov, M.P.

    1974-08-03

    An examination was made of the meristic and morphological characters of underyearlings of the main commercial species, namely roach (Rutilus rutilus), bream (Abramis brama), perch (Perca fluviatilis) and pike (Esox lucius) from the zone affected by waters discharged from Konakovo power station into Ivan'kovskoye reservoir. These characters were compared with the morphological characteristics of underyearlings from a control bay. The results showed that there were no significant differences between the meristic characters of the species studied. This is connected with the earlier spawning time of the fish, as a result of which embryogeny and development of the young occurred at temperatures similar to those in the control and around the optimum for each species. The morphological characters of roach and bream underyearlings from the warm water zone and the control area did differ. There wre differences in the size of the paired and unpaired fins and in the ventroanal distance. In addition, greatest and least body depth were increased in roach underyearlings of the warm water zone, while the depth of the head at the occiput, the length of the caudal peduncle and the base length of the anal fin were increased in the bream. There were no significant differences in perch and pike underyearlings. (FMM)

  18. First detection of circovirus-like sequences in amphibians and novel putative circoviruses in fishes.

    Science.gov (United States)

    Tarján, Zoltán László; Pénzes, Judit J; Tóth, Róza P; Benkő, Mária

    2014-03-01

    The negative samples of a collection, established originally for seeking new adeno- and herpesviruses in lower vertebrates, were screened for the pres-ence of circoviruses by a consensus nested PCR targeting the gene coding for the replication-associated protein. Six fish samples representing five species, namely asp (Aspius aspius), roach (Rutilus rutilus), common bream (Abramis brama), round goby (Neogobius melanostomus) and monkey goby (Neogobius fluviatilis), as well as three frog samples were found positive for circoviral DNA. Sequence analysis of the amplicons indicated the presence of three novel putative circo-like viruses and a circovirus in Hungarian fishes and one novel circovirus in a common toad (Bufo bufo), and another one in a dead and an alive specimen of green tree frog (Litoria caerulea), respectively. In phylogeny reconstruction, the putative bream circovirus clustered together with circoviruses discovered in other cyprinid fishes recently. Three other piscine circoviral sequences appeared closest to sequences derived from different environmental samples. Surprisingly, the nucleotide sequence derived from two fish samples (a bream and a monkey goby) proved to be from porcine circovirus 2 (PCV2), almost identical to a sequence detected in Sweden previously. This is the first report on the detection of PCV2 in fish and circoviral DNA in amphibian hosts. PMID:24334078

  19. 超声波辅助提取榛子油的工艺条件优化%Optimizing Condition of Ultrasonic-Assistied Extraction of Hazelnut Seed Oil

    Institute of Scientific and Technical Information of China (English)

    杨青珍; 王锋; 李康

    2011-01-01

    To determine the effects of different hammer mill screen sizes on feed processing characteristics, growth and digestive enzyme activity of blunt snout bream(Megalobrama amblycephala) ,450 breams were randomly allocated to 3 treatments with 5 replicates and fed with the diets ground through three screen mesh sizes (0. 8,1. 0 and 1.2 mm) respectively before pelleting. Results showed that;Screen mesh size was positively correlated with mill production, and negatively correlated with electricity consumption. The hardness of the diets through 1. 2 mm mesh size was the lowest or much lower than the other two groups. The diet through 0. 8 mm mesh size had a higher leaching rate than that through 1. 0 mm mesh size and its starch gelatinization was much higher than that of the other two groups. The breams fed with the diet through 1.0 mm mesh size had numerically the highest weight gain and growth rate, and its specific growth rate was higher than those fed with the diet through 0. 8 mm mesh size. The diet through 0. 8 mm mesh size resulted in a higher condition factor of breams than that through 1.2 mm mesh size. There was no effect of hammer mill screen size on hepatopancreas index, relative length of intestine or digestive enzyme activity. In conclusion, it might be better to grind the feed of blunt snout bream through 1.0 mm mesh size.%以榛子为原料,利用超声波辅助提取榛子油.在单因素试验的基础上,通过正交试验确定了超声波辅助提取榛子油的最佳工艺条件.结果表明:在试验范围内,各因素对榛子油得率的影响大小顺序为提取温度>提取时间>超声波功率>液料比.以石油醚为溶剂提取榛子油的最佳工艺条件为:液料比为8 mL/g、提取温度为60℃、超生波功率为500 W、提取时间为60 min,榛子油得率为74.89%.

  20. Phylogeny of Trachylepis sp. (Reptilia) from Turkey inferred from mtDNA sequences.

    Science.gov (United States)

    Güçlü, Ozgür; Candan, Kamil; Kankiliç, Tolga; Kumlutaş, Yusuf; Durmuş, Salih Hakan; Poulakakis, Nikos; Ilgaz, Cetin

    2014-12-01

    The taxonomic status of the species included into the genus Trachylepis in Turkey are doubtful. So far, three morphological species have been attributed to this genus in Turkey; Trachylepis aurata. T. vittata, and T. septemtaeniata. Here, we investigated the taxonomy of the Turkish Trachylepis species by employing phylogenetic and phylogeographic approaches and using mitochondrial DNA (cytochrome b and 12 S rRNA). In total, 45 Trachylepis and 6 Mabuya specimens were used analyzed. Phylogenetic analyses were carried out using Maximum Likelihood (ML) and Bayesian Inference (BI) methods. The phylogenetic relationships and the genetic distances retrieved, revealed that the Turkish species, which currently recognized as Trachylepis, are highly diversified, forming a distinct clade that shows closer phylogenetic affinity with the species of the genus Mabuya rather than the other Trachylepis species. In this clade, the three Turkish species are monophyletic with T. vittata to branch off first in late Miocene (10.54 Mya). The other two species (T. septemtaeniata and T. aurata) seem to have sister group relationship that diverged at the end of Messinian Salinity Crisis (5.27 Mya). As a whole, the examination of mtDNA lineages in the Turkish lizards of the genus Trachylepis may contribute substantially to the refining of their taxonomic status, since the three species of Turkey, although monophyletic, represent a distinct radiation that would could probably recognized as a different genus in Mabuya sensu lato. PMID:24004307

  1. Assessment of heavy metal contamination levels and toxicity in sediments and fishes from the Mediterranean Sea (southern coast of Sfax, Tunisia).

    Science.gov (United States)

    Zohra, Ben Salem; Habib, Ayadi

    2016-07-01

    Contamination of heavy metals in sediment is regarded as a global crisis with a large share in industrializing cities like Sfax (Tunisia). Seven heavy metals such as Cadmium (Cd), Copper (Cu), Iron (Fe), Mercury (Hg), Nickel (Ni), Lead (Pb), and Zinc (Zn), and one metalloid such as Arsenic (As) in sediments and fish (D. annularis, L. aurata, and S. vulgaris) were investigated from the Southern coast of Sfax in Tunisia. The range of metals in sediments were 13.11-36; 4.42-7.92; 8.23-28.56; 50,564-11,956; 2.9-6.8; 9.13-30.51; 65.06-151.50, and 47-546 kg(-1) DW for As, Cd, Cu, Fe, Hg, Ni, Pb, and Zn. The level of studied metals in sediment samples exceeded the limits of the quality assessment guidelines (SQGs). The potential ecological risk index (PERI) proved that the investigated region could pose moderate risk for the aquatic biota. Metal bioaccumulation in the fish muscles varied significantly among species. Indeed, S. vulgaris and D. annularis accumulated higher amount of metal than L. aurata. The target hazard quotients (THQ) of individual heavy metals in fish, except for As and Hg, revealed safe levels for human consumption. Nevertheless, the total THQ indexes exceeded 1 suggesting the combined effects on muscles fish, which may constitute a risk to population's health. PMID:27040542

  2. Morphological polymorphism in tapeworms: redescription of Caryophyllaeus laticeps (Pallas, 1781) (Cestoda: Caryophyllidea) and characterisation of its morphotypes from different fish hosts.

    Science.gov (United States)

    Hanzelová, Vladimíra; Oros, Mikuláš; Barčák, Daniel; Miklisová, Dana; Kirin, Diana; Scholz, Tomáš

    2015-02-01

    Recent morphological and molecular data have shown that one of the most common parasites of freshwater fish in the Palaearctic Region, the cestode Caryophyllaeus laticeps (Pallas, 1781) (Eucestoda: Caryophyllidea), is highly polymorphic. Five distinct morphotypes of C. laticeps, largely corresponding to different fish hosts and representing separate, yet closely related genetic lineages, have been recognised and they are characterised in the present paper. Morphotype 1 from breams, Abramis brama (L.) (type-host) and Ballerus spp., corresponds to the original Taenia laticeps Pallas, 1781 and its neotype (paragenophore ex A. brama in Russia) is designated. This morphotype is characterised by a slender body and flabellate scolex. Morphotype 2 was found in the Macedonian vimba Vimba melanops (Heckel) and the vimba bream V. vimba (L.); it is typified by a more robust body, with most anterior extent of the vitelline follicles near the scolex and the cirrus-sac situated more anteriorly than in other morphotypes. Morphotype 3 is represented by worms from the common carp Cyprinus carpio L. that possess a cuneicrispitate scolex (having the form of a wedge with shallow indentations on anterior margin). Morphotype 4 from the common nase Chondrostoma nasus (L.) has a large, robust body and a wide scolex with numerous superficial grooves (wrinkles) in its anterior part. Morphotype 5 is represented by worms from the white-eye bream Ballerus sapa (Pallas); its typical characteristics are a festoon-like anterior margin of the scolex, the absence of vitelline follicles posterior to the cirrus-sac and the absence of a well-developed internal seminal vesicle. Discriminant analysis of 15 morphometric variables readily separated Morphotypes 3, 4 and 5 and confirmed the key discriminating power of traits related to the reproductive system, especially the terminal reproductive organs. Morphological polymorphism and the genetic divergence of different morphotypes of C. laticeps correspond

  3. Effect of monitoring strategies and reference data of the German Environmental Specimen Banking Program

    International Nuclear Information System (INIS)

    The constitution of the German Environmental Specimen Bank (ESB) has started in 1985, subsequent to a successful pilot study concerning the feasibility. Since that time, a multitude of technological and methodical standards have been developed, which allow for a high quality of the storage-samples and of the specimen characterization. While the storage-samples are kept for retrospective analysis, by now, already comprehensive data on the material-developing in the environment are available due to a real time monitoring of selected environmental chemicals over a period of up to twenty years. Thus, spatial and temporal trends can be described. Since the state of knowledge on critical tissue concentrations in the sublethal range is extremely low at present, it is however not possible to accomplish a direct assessment of relevancy of the substance concentrations. Hence, within the scope of the German ESB Program, the following strategies on assessment of relevancy are observed: use of biomarkers, histopathological examinations, biometric specimen characterization, use of ecological indicator groups, and development of a reference system with analytical and biometric data. Thus, for example endocrine effects in male breams in the river Saar, which correlate directly to operational discharges from municipal sewage plants, could be detected. By histopathological examinations, fibrotic and necrotic tissue adaptations on the gonads had been ascertained cumulatively, which unambiguously imply a restricted fertility of the male breams. In the river Rhine, an improved growth along the timeline could be described on the basis of biometric characterization of breams, which is regarded as rate for the reaction to all structural and material changes in the water body. Presently, with the development of a reference system based on the data collected in the scope of the Environmental Specimen Bank, a basis for the assessment of monitoring results with accumulation indicators is

  4. Sinusoidal swimming in fishes: the role of season, density of large zooplankton, fish length, time of the day, weather condition and solar radiation

    Czech Academy of Sciences Publication Activity Database

    Jarolím, Oldřich; Kubečka, Jan; Čech, Martin; Vašek, Mojmír; Peterka, Jiří; Matěna, Josef

    2010-01-01

    Roč. 654, č. 1 (2010), s. 253-265. ISSN 0018-8158 R&D Projects: GA ČR(CZ) GA206/07/1392; GA ČR(CZ) GP206/09/P266; GA AV ČR(CZ) 1QS600170504 Institutional research plan: CEZ:AV0Z60170517 Keywords : common bream Abramis brama * Daphnia * echosounder * Leptodora * roach Rutilus rutilus * Římov Reservoir * Sonar5 Subject RIV: EG - Zoology Impact factor: 1.964, year: 2010

  5. Biomass and abundance biases in European standard gillnet sampling

    Czech Academy of Sciences Publication Activity Database

    Šmejkal, Marek; Ricard, Daniel; Prchalová, Marie; Říha, Milan; Muška, Milan; Blabolil, Petr; Čech, Martin; Vašek, Mojmír; Jůza, Tomáš; Herreras, A.M.; Encina, L.; Peterka, Jiří; Kubečka, Jan

    2015-01-01

    Roč. 10, č. 3 (2015), e0122437. E-ISSN 1932-6203 R&D Projects: GA MŠk(CZ) EE2.3.20.0204; GA ČR(CZ) GPP505/12/P647; GA MŠk(CZ) EE2.3.30.0032 Institutional support: RVO:60077344 Keywords : fish sampling * gillnets * large meshes * mesh size selectivity * Improvement of European standard EN 14757 * bream (Abramis brama ) Subject RIV: GL - Fishing Impact factor: 3.234, year: 2014

  6. Rybářství v nádržích s rozdílným ekohydrologickým režimem

    Czech Academy of Sciences Publication Activity Database

    Draštík, Vladislav; Kubečka, Jan; Šovčík, P.

    Vodňany : Jihočeská univerzita, Výzkumný ústav rybářský a hydrobiologický, 2004 - (Vykusová, B.), s. 180-185 ISBN 80-85887-50-9. [Česká ichtyologická konference /7./. Vodňany (CZ), 06.05.2004-07.05.2004] R&D Projects: GA ČR(CZ) GA206/02/0520 Keywords : anglers catch * fish species composition * bream/perch ratio Subject RIV: DA - Hydrology ; Limnology

  7. Megalocytiviruses

    Directory of Open Access Journals (Sweden)

    Kazuhiro Nakajima

    2012-04-01

    Full Text Available The genus Megalocytivirus, represented by red sea bream iridovirus (RSIV, the first identified and one of the best characterized megalocytiviruses, Infectious spleen and kidney necrosis virus (ISKNV, the type species of the genus, and numerous other isolates, is the newest genus within the family Iridoviridae. Viruses within this genus are causative agents of severe disease accompanied by high mortality in multiple species of marine and freshwater fish. To date outbreaks of megalocytivirus-induced disease have occurred primarily in south-east Asia and Japan, but infections have been detected in Australia and North America following the importation of infected ornamental fish. The first outbreak of megalocytiviral disease was recorded in cultured red sea bream (Pagrus major in Japan in 1990 and was designated red sea bream iridovirus disease (RSIVD. Following infection fish became lethargic and exhibited severe anemia, petechiae of the gills, and enlargement of the spleen. Although RSIV was identified as an iridovirus, sequence analyses of RSIV genes revealed that the virus did not belong to any of the four known genera within the family Iridoviridae. Thus a new, fifth genus was established and designated Megalocytivirus to reflect the characteristic presence of enlarged basophilic cells within infected organs. Indirect immunofluorescence tests employing recently generated monoclonal antibodies and PCR assays are currently used in the rapid diagnosis of RSIVD. For disease control, a formalin-killed vaccine was developed and is now commercially available in Japan for several fish species. Following the identification of RSIV, markedly similar viruses such as infectious spleen and kidney necrosis virus (ISKNV, dwarf gourami iridovirus (DGIV, turbot reddish body iridovirus (TRBIV, Taiwan grouper iridovirus (TGIV, and rock bream iridovirus (RBIV were isolated in East and Southeast Asia. Phylogenetic analyses of the major capsid protein (MCP and

  8. NPY and sbGnRH gene expression in juvenile and adult male Brazilian flounder Paralichthys orbignyanus Expressão gênica do NPY e do sbGnRH em machos juvenis e adultos de linguado Paralichthys orbignyanus

    OpenAIRE

    Vinicius Farias Campos; Tiago Veiras Collares; Fabiana Kömmling Seixas; João Carlos Deschamps; Luis Fernando Fernandes Marins; Marcelo Hideo Okamoto; Luís André Nassr Sampaio; Ricardo Berteaux Robaldo

    2011-01-01

    The objective of this study was to evaluate neuropeptide Y (NPY) and sea bream gonadotropin-release hormone (sbGnRH) gene expression in juvenile and adult males of Brazilian flounder. Hypothalamuses from fish were sampled for total RNA extraction. After cDNA synthesis, real-time PCR was used to measure gene expression. NPY showed approximately 2-fold increases in their mRNA levels while sbGnRH showed 3-fold increases in adult fish. These results suggest that these peptides could be involved o...

  9. Present status of fish culture using warm waste water from power plants

    International Nuclear Information System (INIS)

    The research of fish culture using warm waste water from power stations in Japan has history of over ten years. It is being gradually commercialized, but still various problems remain. Among the fish culture activities, those related to nuclear power generation are described as follows: Tokai ponds of Warm Water Fish Culture Development Society of Japan (culturing crimson sea bream, ear shell, flatfish, prawn, and eel); Fukui Prefectural Fisheries Experimental Station (culturing sweetfish and young yellowtail); and, Warm Water Utilization Center of Shizuoka Prefecture (culturing ear shell). (Mori, K.)

  10. Inter- and size-specific patterns of fish seasonal migration between a shallow lake and its streams

    DEFF Research Database (Denmark)

    Skov, Christian; Brodersen, J.; Nilsson, P.A.; Hansson, L.A.; Brönmark, C.

    2008-01-01

    cues. The cyprinid fishes generally entered the streams in late afternoon or in the morning, depending on season, but overall migration patterns varied between the three streams. We suggest and discuss that our results have great implications for lake management as well as for the interpretation of...... perch (Perca fluviatilis (L.))) between a shallow lake and its streams. Migration patterns were investigated from October to June, and a substantial part of the roach (40%) and white bream (55%) populations tagged in the lake during autumn migrated during winter into the streams, whereas only very few...... seasonal trophic dynamics in shallow lakes....

  11. The Addition of Hydrocolloids (Carboxymethylcellulose, Alginate and Konjac) to Improve the Physicochemical Properties and Sensory Characteristics of Fish Sausage Formulated with Surimi Powder

    OpenAIRE

    Santana, Palestina; Huda, Nurul; Yang, Tajul Aris

    2013-01-01

    Fish sausages were prepared using threadfin bream (Nemipterus japonicus) surimi powder, to which 0.5% carboxymethylcellulose (sCMC), 0.5% alginate (ALG), 0.5% konjac (KJC), or no hydrocolloid (SP) was added. A fifth batch was prepared using frozen surimi (FS). The physicochemical properties and sensory characteristics of each sausage type were analyzed. All samples received a grade of 5 in the folding test. However, sCMC, ALG, and KJC had significantly higher (P<0.05) hardness, springi...

  12. An attempt to analyze fish otoliths by in-air PIXE

    Science.gov (United States)

    Arai, Nobuaki; Sakamoto, Wataru; Maeda, Kuniko

    1996-04-01

    Fish otoliths are generally composed of aragonite crystals. A large number of trace elements such as Sr, Fe, Mn, and Zn are included in the crystal. Especially, Sr enters the crystals much among those metals in the process of precipitation of the calcium carbonate. In addition, it is expected that Sr/Ca concentration ratios can indicate ambient temperature. In-air PIXE was adopted to analyze trace elements in otoliths of juvenile red sea bream. Preliminary results indicated that the Sr/Ca ratios of the otoliths increased proportional to rearing temperature and that PIXE was a fast and convenient technique for determination of several elements in fish otoliths.

  13. Autonomous Underwater Navigation and Optical Mapping in Unknown Natural Environments

    Directory of Open Access Journals (Sweden)

    Juan David Hernández

    2016-07-01

    Full Text Available We present an approach for navigating in unknown environments while, simultaneously, gathering information for inspecting underwater structures using an autonomous underwater vehicle (AUV. To accomplish this, we first use our pipeline for mapping and planning collision-free paths online, which endows an AUV with the capability to autonomously acquire optical data in close proximity. With that information, we then propose a reconstruction pipeline to create a photo-realistic textured 3D model of the inspected area. These 3D models are also of particular interest to other fields of study in marine sciences, since they can serve as base maps for environmental monitoring, thus allowing change detection of biological communities and their environment over time. Finally, we evaluate our approach using the Sparus II, a torpedo-shaped AUV, conducting inspection missions in a challenging, real-world and natural scenario.

  14. Autonomous Underwater Navigation and Optical Mapping in Unknown Natural Environments.

    Science.gov (United States)

    Hernández, Juan David; Istenič, Klemen; Gracias, Nuno; Palomeras, Narcís; Campos, Ricard; Vidal, Eduard; García, Rafael; Carreras, Marc

    2016-01-01

    We present an approach for navigating in unknown environments while, simultaneously, gathering information for inspecting underwater structures using an autonomous underwater vehicle (AUV). To accomplish this, we first use our pipeline for mapping and planning collision-free paths online, which endows an AUV with the capability to autonomously acquire optical data in close proximity. With that information, we then propose a reconstruction pipeline to create a photo-realistic textured 3D model of the inspected area. These 3D models are also of particular interest to other fields of study in marine sciences, since they can serve as base maps for environmental monitoring, thus allowing change detection of biological communities and their environment over time. Finally, we evaluate our approach using the Sparus II, a torpedo-shaped AUV, conducting inspection missions in a challenging, real-world and natural scenario. PMID:27472337

  15. ONE NEW SPECIES THE GENUS EURYHALIOTREMA (MONOGENEA, ANCYROCEPHALIDAE)%宽海盘虫属(单殖亚纲,锚首虫科)一新种记述

    Institute of Scientific and Technical Information of China (English)

    李海燕

    2006-01-01

    记述了寄生于广东海洋鱼类紫红笛鲷Lutjanus argentimaculatus(Forskal)和黑鲷Sparus macrocephalus(Basilewsky)鳃上的宽海盘虫属Euryhaliotrema Kritsky & Boeer,2002一新种,新种笛鲷宽海盘虫Eruyhaliotrema lutjanisp.nov.的中央大钩与联结片的形状相似于Euryhaliotrema carbunculus(Hargis,1955)Kritsky&Boeger,2002和广东宽海盘虫Euryhaliotrema guangdongensis Li et al.,2005,但交接管形状及量度不同,同时支持器的形状与已知种差别较大.

  16. 两种宽海盘虫(包括一新种)的描述%Description of two species (including a new one) in Euryhaliotrema Kritsky & Boeger, 2002

    Institute of Scientific and Technical Information of China (English)

    李海燕

    2005-01-01

    记述了寄生于海洋鱼类黑鲷Sparus macrocephalus(Basilewsky)鳃上的宽海盘虫属Euryhaliotrema Kritsky & Boeger,2002一新种和寄生于金带笛鲷Lutjanus vaigiensis(Cuoy & Gaimard)鳃上的约氏宽海盘虫Euryhaliotrema johnii Kritsky & Boeger,2002.广州宽海盘虫,新种Euryhaliotrema guang-zhouensis sp.nov.的腹联结片和交接管的形状与Eruyhaliotrema potamocetema Kritsky & Boeger,2002,E.paralonchuvi Kritsky & Boeger,2002和E. atlantica Kritsky & Boeger,2002相似,交接管呈螺旋形,均环绕两圈半,但它们的支持器形状差异较大,且背中央大钩和联结片的形状和量度都不同.

  17. Aerobic capacity influences the spatial position of individuals within fish schools

    DEFF Research Database (Denmark)

    Killen, Shaun S.; Marras, Stefano; Steffensen, John Fleng; McKenzie, David

    2012-01-01

    of juvenile mullet Liza aurata were filmed at three swim speeds in a swim tunnel, with one focal fish from each school then also measured for standard metabolic rate (SMR), maximal metabolic rate (MMR), aerobic scope (AS) and maximum aerobic swim speed. At faster speeds, fish with lower MMR and AS...... swam near the rear of schools. These trailing fish required fewer tail beats to swim at the same speed as individuals at the front of schools, indicating that posterior positions provide hydrodynamic benefits that reduce swimming costs. Conversely, fish with high aerobic capacity can withstand...... increased drag at the leading edge of schools, where they could maximize food intake while possibly retaining sufficient AS for other physiological functions. SMR was never related to position, suggesting that high maintenance costs do not necessarily motivate individuals to occupy frontal positions. In the...

  18. Anchoring novel molecular biomarker responses to traditional responses in fish exposed to environmental contamination

    Energy Technology Data Exchange (ETDEWEB)

    Nogueira, Patricia [CESAM and Departamento de Biologia, Universidade de Aveiro, Campus de Santiago, 3810-193 Aveiro (Portugal); Department of Biology and Environmental Science, University of Sussex, Falmer, Brighton BN1 9QJ (United Kingdom); Pacheco, Mario [CESAM and Departamento de Biologia, Universidade de Aveiro, Campus de Santiago, 3810-193 Aveiro (Portugal); Lourdes Pereira, M. [CICECO and Departamento de Biologia, Universidade de Aveiro, Campus de Santiago, 3810-193 Aveiro (Portugal); Mendo, Sonia [CESAM and Departamento de Biologia, Universidade de Aveiro, Campus de Santiago, 3810-193 Aveiro (Portugal); Rotchell, Jeanette M., E-mail: J.Rotchell@sussex.ac.u [Department of Biology and Environmental Science, University of Sussex, Falmer, Brighton BN1 9QJ (United Kingdom)

    2010-05-15

    The responses of Dicentrarchus labrax and Liza aurata to aquatic pollution were assessed in a contaminated coastal lagoon, using both traditional and novel biomarkers combined. DNA damage, assessed by comet assay, was higher in both fish species from the contaminated sites, whereas levels of cytochrome P450 1A1 gene expression were not significantly altered. The liver histopathological analysis also revealed significant lesions in fish from contaminated sites. Alterations in ras and xpf genes were analysed and additional pollutant-responsive genes were identified. While no alterations were found in ras gene, a downregulation of xpf gene was observed in D. labrax from a contaminated site. Suppression subtractive hybridization applied to D. labrax collected at a contaminated site, revealed altered expression in genes involved in energy metabolism, immune system activity and antioxidant response. The approach and results reported herein demonstrate the utility of anchoring traditional biomarker responses alongside novel biomarker responses. - Novel molecular biomarkers of aquatic environmental contamination in fish.

  19. “Sacred scarab” in jewelry of Ancient Egypt: the symbol interpretation problem

    Directory of Open Access Journals (Sweden)

    Lada V. Prokopovich

    2015-03-01

    Full Text Available The image of the Sacred scarab, like any other fact of culture, should be considered only in the context of certain circumstances, which involve the approach choice: viewing as either a sign or a symbol. In those cases where the scarab image stands as a symbol, while interpreting this one it should be taken into account the cultural context, which, as relevant studies show, can never be reduced to a simple interpretation scheme “Scarab — Dorbeetle — Sun symbol”. In that connection suggested is a hypothesis, according to which the image of the Sacred scarab as a symbol is divided into two, at least, concepts: a “Sunny Beetle”, to mean the beetle species Cetonia aurata (or any other similar, and a “Beetle-Demiurge”, to mean the dorbeetle.

  20. Partitioning the metabolic scope: the importance of anaerobic metabolism and implications for the oxygen- and capacity-limited thermal tolerance (OCLTT) hypothesis

    DEFF Research Database (Denmark)

    Ejbye-Ernst, Rasmus; Tirsgaard, B.; Michaelsen, Thomas Y.;

    2016-01-01

    Ongoing climate change is predicted to affect the distribution and abundance of aquatic ectotherms owing to increasing constraints on organismal physiology, in particular involving the metabolic scope (MS) available for performance and fitness. The oxygen- and capacity-limited thermal tolerance....... aurata. In contrast, both species revealed significant negative correlations between aerobic and anaerobic components of the MS, indicating a trade-off where both components of the MS cannot be optimized simultaneously. Importantly, the fraction of the MS influenced by anaerobic metabolism was on average...... performance. Our results suggest that without accounting for anaerobic metabolism within the MS, studies involving the OCLTT hypothesis could overestimate the metabolic scope available for sustainable activities and the ability of individuals and species to cope with climate change...

  1. Mueller-matrix ellipsometry studies of optically active structures in scarab beetles

    Directory of Open Access Journals (Sweden)

    Arwin H.

    2010-06-01

    Full Text Available The complexity of multilayers, photonic crystals, metamaterials and other artificial materials has promoted the use of spectroscopic, variable angle, generalized and Mueller-matrix ellipsometry. Naturally occurring structures may show even higher complexity than artificial structures but with a more narrow range of constituent materials. Fascinating reflection properties result from intricate photonic structures in, for instance, the wing scales and cuticles of insects. Currently there is a large interest to explore such functional supramolecular architectures for exploitation in nanotechnology. In this study, Mueller-matrix spectroscopic ellipsometry is applied in the spectral range of 250 to 1000 nm to investigate optical response and structures of the cuticle of Scarab beetles of the Cetoniinae subfamily. The cuticle of Cetonia aurata (the rose chafer, la cétoine dorée is green with a metallic appearance and reflects left-handed circular/elliptically polarized light. It has been suggested that the polarization of this metallic gloss is caused by a helical structure in the chitinous cuticle. We find that the polarization effect is limited to the narrow spectral range 470-550 nm whereas for shorter or longer wavelengths the reflection properties are similar to those from a near-dielectric material. Model calculations and parameterization of the nanostructure employing a heliocoidal structure are discussed. As a comparison the polarization effects from light reflected from two other beetles will be presented. Coptomia laevis has a similar appearance as Cetonia aurata but has very different polarization properties. The golden Plusiotis argentiola has very interesting properties showing both left and right-handed polarization depending on incidence angle and wavelength.

  2. High prevalence of trypanosome co-infections in freshwater fishes.

    Science.gov (United States)

    Grybchuk-Ieremenko, Anastasiia; Losev, Alexander; Kostygov, Alexei Yu; Lukeš, Julius; Yurchenko, Vyacheslav

    2014-12-01

    One thousand three hundred seventy three fish specimens of eight different species from the vicinity of Kyiv, Ukraine, were examined for the presence of trypanosomes and 921 individuals were found to be infected. The prevalence of infection ranged from 24% in freshwater bream, Abramis brama (Linnaeus), to 100% in spined loach, Cobitis 'taenia' Linnaeus. The level of parasitaemia also varied significantly between generally mild infections in pikeperch, Sander lucioperca (Linnaeus), and heavy ones in C. 'taenia'. In most cases the infections with trypanosomes were asymptomatic. Cases of co-infection with species of Trypanoplasma Laveran et Mesnil, 1901 were documented for five out of eight examined host species. Molecular analysis of the 18S rDNA sequences revealed that four hosts, namely northern pike, Esox lucius Linnaeus, freshwater bream, spined loach and European perch, Perca fluviatilis Linnaeus, were simultaneously infected with two different trypanosome species. Our findings advocate the view that to avoid the risk posed by mixed infections, subsequent molecular taxonomic studies should be performed on clonal lines derived from laboratory cultures of fish trypanosomes. PMID:25651690

  3. Longitudinal and Vertical Spatial Gradients in the Distribution of Fish within a Canyon-shaped Reservoir

    Science.gov (United States)

    Vaek, Mojmír; Kubeka, Jan; Peterka, Jií; Ech, Martin; Dratík, Vladislav; Hladík, Milan; Prchalová, Marie; Frouzová, Jaroslava

    2004-09-01

    The large-scale spatial distribution of fish was investigated within a morphometrically simple canyon-shaped reservoir with a single major tributary and a longitudinal trophic gradient (ímov Reservoir, Czech Republic). Samples of fish were taken by Nordic survey gill nets (several mesh sizes from 8 to 70 mm knot to knot) installed as surface nets at several offshore areas located along the longitudinal axis of the reservoir. Surveys were carried out in late summer during 1999-2003. An obvious distribution gradient of fish was revealed along the longitudinal axis of the ímov Reservoir. The total relative fish abundance and biomass (catch per unit effort) decreased considerably from the upstream end of the reservoir toward the dam. Roach (Rutilus rutilus), bleak (Alburnus alburnus) and bream (Abramis brama) comprised the bulk of catches at all areas. Enhanced dominance of bream was observed in the fish assemblage at the uppermost, more eutrophic area of the reservoir. The highest number of fish species and the highest abundance of young-of-the-year fish were also observed in the tributary area. In the downstream part of the reservoir, gill net surveys along the vertical depth profiles indicated that offshore fish occupied mostly the epilimnion. Extreme flood events affected the ímov Reservoir, however, it seemed they had no significant impact on the gradients described. (

  4. Contamination of Omnivorous Freshwater Fish Species and Sediments by Chlorinated Hydrocarbons in Poland

    Directory of Open Access Journals (Sweden)

    Niewiadowska Alicja

    2014-10-01

    Full Text Available The occurrence and concentrations of organochlorine pesticides and polychlorinated biphenyls (PCBs were determined in 158 muscle samples of bream (Abramis brama and roach (Rutilus rutilus, and 84 samples of sediments collected from 10 river and lake sampling sites in 2011 and 2012. The concentrations of DDTs (p,p’-DDT, o,p’-DDT, p,p’-DDE, and p,p’-DDD, HCH isomers (a-, ß-, and y-HCH, HCB, and PCBs (six indicator PCB congeners 28, 52, 101, 138, 153 and 180 were determined using the capillary gas chromatography. The mean concentrations of DDTs in bream and roach were in the range of 11.2-654 and 4.5-121 ug/kg wet weight respectively, and PCBs were in the range of 1.3-75.9 and 1.1-112 ug/kg wet weight, respectively. Mean concentrations of DDTs and PCBs in sediments were 0.5-270 ug/kg dry weight and ⋋0.1-2.2 ug/kg dry weight respectively. The study showed clear spatial differences in the levels of organochlorine pesticides and PCBs in fish and sediments from different aquatic ecosystems. The highest levels of contaminants were detected in fish and sediments from the Vistula River in vicinity of Cracow. The possible risk to the fish meat consumers and ecological risk were evaluated.

  5. The role of the Environmental Specimen Bank in environmental monitoring: The example of CHC in selected matrices; Die Rolle der Umweltprobenbank in der Umweltueberwachung am Beispiel von chlorierten Kohlenwasserstoffen in ausgewaehlten Matrizes

    Energy Technology Data Exchange (ETDEWEB)

    Marth, P.; Kettrup, A. [GSF - Forschungszentrum fuer Umwelt und Gesundheit Neuherberg GmbH, Oberschleissheim (Germany). Inst. fuer Oekologische Chemie]|[Technische Univ. Muenchen, Freising (Germany). Lehrstuhl fuer Oekologische Chemie und Umweltanalytik; Schramm, K.W.; Henkelmann, B.; Wolf, A.; Oxynos, K.; Schmitzer, J. [GSF - Forschungszentrum fuer Umwelt und Gesundheit Neuherberg GmbH, Oberschleissheim (Germany). Inst. fuer Oekologische Chemie

    1999-03-01

    For precautionary environmental protection the Environmental Specimen Bank (ESB) has been successfully established as a permanent environmental surveillance tool in Germany. The presented monitoring results on chlorinated hydrocarbons (CHC) show very clearly the effects of political decisions and can be used for the identification of pollutant sources. The CHC burden of breams (Abramis brama) from five German rivers (Elbe, Rhein, Saar, Mulde, Saale) gives an current overview of the pollution situation of different limnic ecosystems in Germany. A calculation model about the distribution of organic pollutants between different tissues offers the possibility to predict concentrations in liver and muscle of breams. Legislative emission restrictions have led to a significant decrease of dioxin levels in herring gull (Larus argentatus) eggs between 1988-1996. (orig.) [Deutsch] Die Umweltprobenbank des Bundes hat sich im Rahmen der Oekologischen Umweltbeobachtung zu einem wichtigen und permanenten Instrument der Umweltpolitik entwickelt. Neben der Langzeitkonservierung fuer zukuenftige Forschungsaufgaben wird am Beispiel der chlorierten Kohlenwasserstoffe demonstriert, dass das durchgefuehrte Monitoring-Programm als Nachweis fuer die Effektivitaet gesetzlicher Massnahmen und zur Identifizierung von Schadstoffeintragsquellen genutzt werden kann. Der vorliegende Beitrag vermittelt am Beispiel der Brassen (Abramis brama) als Akkumulationsindikator einen aktuellen und raeumlich differenzierten Ueberblick ueber die chlororganische Belastungssituation bundesdeutscher Gewaesser (Elbe, Rhein, Saar, Mulde, Saale). Betrachtungen zur Verteilung organischer Chemikalien zwischen Gewebetypen von Brassen zeigen, dass sich die Verteilung der Schadstoffkonzentrationen in Leber und Muskel mit einem geringen Fehler errechnen laesst. Die Dioxingehalte von Silbermoeweneiern (Larus argentatus) belegen, dass die Belastung durch strengere Emissionsgrenzwerte seit Beginn der neunziger Jahre

  6. Occurrence of priority and emerging organic compounds in fishes from the Rhone River (France).

    Science.gov (United States)

    Miège, C; Peretti, A; Labadie, P; Budzinski, H; Le Bizec, B; Vorkamp, K; Tronczyński, J; Persat, H; Coquery, M; Babut, M

    2012-11-01

    The main objective of this study was to collect new data on the occurrence, levels of priority and emerging organic compounds in freshwater fish sampled in the Rhone River. The 34 studied contaminants included alkylphenols, bisphenol A, polybromodiphenylethers (PBDE), perfluorinated compounds, hexabromocyclododecanes (HBCD), hexachlorobenzene and hexachlorobutadiene (HCBD). About 50 fish samples (individual specimens or pooled fish) were collected from three sites located upstream and downstream of the Lyon metropolitan area in the Rhone River (France). Four species were caught at each site, namely: the barbel (Barbus barbus), the common bream (Abramis brama), the white bream (Blicca bjoerkna) and the chub (Squalius cephalus). Some contaminants were quantified in all the 32 fish samples analysed: 4-nonylphenol, α-HBCD, the six PBDE congeners (28, 47, 99, 100, 153, 154), perfluorooctanesulfonate (PFOS) and perfluorodecanoic acid. Twenty three of the 32 samples had a concentration of PFOS above the Environmental Quality Standards (EQS) (up to six times higher than the EQS), and all the 32 samples had concentrations of PBDE above the EQS (up to 4,000 times higher, with the sum of six PBDE varying from 4.5 to 182 ng/g dry weight). Clearly, the interest to consider PFOS and HBCD as new priority substances is confirmed. In contrast, the pertinence of a priority status for HCBD, which was never quantified in our study, might have to be reconsidered in the future. PMID:22760502

  7. Concentrations of 17 elements in muscle, gills, liver and gonads of five economically important fish species from the Danube River

    Directory of Open Access Journals (Sweden)

    Lenhardt M.

    2013-01-01

    Full Text Available The Danube River in the vicinity of the city of Belgrade receives large amounts of untreated or poorly treated communal and industrial waste waters. The aim of this study was to assess elemental accumulation patterns in a number of economically important fish species in this area that belong to different trophic levels. Concentrations of 17 elements (Al, As, B, Ba, Cd, Co, Cr, Cu, Fe, Li, Mn, Mo, Ni, Pb, Se, Sr and Zn were assessed in liver, muscle, gills and gonads of silver carp (Hypophthalmichthys molitrix, freshwater bream (Abramis brama, white bream (Blicca bjoerkna, common carp (Cyprinus carpio and wels catfish (Silurus glanis from the Danube River in Serbia by the use of ICP-OES. Silver carp specimens were differentiated from the other four species by high concentrations of Al and Fe in the liver. Common carp specimens were differentiated by high concentrations of Zn in gills, muscle and liver. Distribution of elements among different tissues had a consistent pattern among the species. Concentrations of Pb, Cd, As, Zn, Cu and Fe in muscle were at acceptable levels for human consumption, while concentrations of Fe and Zn were above maximum acceptable concentrations in liver and gonads.

  8. Cormorant predation on PIT-tagged lake fish

    Directory of Open Access Journals (Sweden)

    Christian Skov

    2014-02-01

    Full Text Available The present study use data from recovered PIT (Passive Integrated Transponder tags to explore species- and size-specific annual predation rates by cormorants on three common lacustrine fishes (size range 120-367 mm in a European lake; roach (Rutilus rutilus, common bream (Abramis brama and perch (Perca fluviatilis. In addition, we quantify the level of age/size truncation that cormorant predation could introduce in a population of perch, an important fish for recreational angling as well as for trophic interactions and ecosystem function in European lakes. Based on three years of PIT tagging of fish in lake Viborg and subsequent recoveries of PIT tags from nearby cormorant roosting and breeding sites, we show that cormorants are major predators of roach, bream and perch within the size groups we investigated and for all species larger individuals had higher predation rates. Perch appear to be the most vulnerable of the three species and based on a comparison with mortality estimates from lakes without significant avian predation, this study suggest that predation from cormorants can induce age/size truncation in lake Viborg, leaving very few larger perch in the lake. This truncation reduces the likelihood of anglers catching a large perch and may also influence lower trophic levels in the lake and thus turbidity as large piscivorous perch often play an important structuring role in lake ecosystem functioning.

  9. CHARACTERIZATION OF LACTATE DEHYDROGENASE ISOZYME PATTERN AND MORPHOLOGY OF THREE MARINE FISH CELL LINES

    Institute of Scientific and Technical Information of China (English)

    郭华荣; 张士璀; 李红岩; 童裳亮; 相建海

    2002-01-01

    Three continuous marine fish cell lines of FG (i.e. , Flounder Gill) from flounder (Paralichthys olivaceus) gill, SPH (i.e., Sea Perch Heart) fro m sea perch (Lateolabrax japonicus) heart and RSBF (i.e., Red Sea Bream Fin) from red se a bream (Pagrosomus major) fin, were characterized by lactate dehydrogenase (LDH) is ozyme and morphological analysis. The LDH isozyme patterns of these three cell lines and their corresponding tissues of origin were investigated and compared. The results sho wed: (1) No difference was found in the LDH isozyme patterns of FG and flounder gill tissue. However, the LDH isozyme patterns of SPH and RSBF were significantly different from their cor responding tissues of origin; (2) LDH isozyme patterns of FG, SPH and RSBF were markedly di fferent from each other and could serve as genetic markers for species identification and de tection of cross contamination. Morphological change analysis of these three cell lines in compa rison to their original tissues indicated that FG cells still appeared epithelioid without mor phological transformation. However, morphological changes were found in SPH and RSBF compa red to their original tissues. Therefore, the cellular morphology was still plastic in the relatively stable culture conditions, and it was possible that change of LDH patterns was related to morphological changes of fish cells in vitro.

  10. Experimental studies on the life-cycle of Sebekia wedli (Pentastomida: Sebekidae).

    Science.gov (United States)

    Junker, K; Boomker, J; Booyse, D G

    1998-12-01

    Four young Nile crocodiles (Crocodylus niloticus) were infected with infective pentastome larvae obtained from naturally infected Mozambique bream, Oreochromis mossambicus, and red-breasted bream, Tilapia rendalli swierstrai in the Kruger National Park. At day 95 post infection one of the crocodiles died and three female and four male S. wedli were recovered from its lungs. One pair was found in copula but the uteri of the females were not yet developed. Males and females were of about equal size. After 226 d post infection the three remaining crocodiles were sacrificed. Two of these harboured no pentastomes but eight were taken from the lungs of the third. The sex ratio had shifted in favour of the females, seven females and one male being present. One of the females recovered from the crocodiles was placed in saline and produced 3,400 eggs. These were used to infect eight guppies, Poecilia reticulata. Within 31 d two infective stages of S. wedli had developed in one of the guppies thus completing the life-cycle of the pentastome. S. wedli recovered from experimentally infected final hosts were slightly smaller than those recovered from a wild-caught final host. PMID:10192834

  11. Molecular characterization of carnitine palmitoyltransferase IA in Megalobrama amblycephala and effects on its expression of feeding status and dietary lipid and berberine.

    Science.gov (United States)

    Lu, Kang-Le; Zhang, Ding-Dong; Wang, Li-Na; Xu, Wei-Na; Liu, Wen-Bin

    2016-01-01

    Carnitine palmitoyltransferase I (CPT I, EC 2.3.1.21) controls the main regulatory step of fatty acid oxidation, and hence studies of its molecular characterization are useful to understand lipid metabolism in cultured fish. Here, a full-length cDNA coding CPT I was cloned from liver of blunt snout bream Megalobrama amblycephala. This cDNA obtained covered 2499bp with an open reading frame of 2181bp encoding 726 amino acids. This CPT I mRNA predominantly expressed in heart and white muscle, while little in eye and spleen. The phylogenetic tree constructed on the basis of sequence alignments among several vertebrate species suggests that this blunt snout bream CPT I sequence belongs to the CPT IA family. In order to investigate the characterization of CPT IA mRNA expression, post-prandial experiment and feeding trial were conducted. The results showed that CPT IA mRNA expression was unchanged from 2 to 12h, and then significantly increased at 24h post-feeding in liver and heart. Berberine, an alkaloid, was identified as a promising lipid-lowering drug. In order to elucidate the effect of berberine on CPT I expression, fish were fed for 8 weeks with three diets (low-fat diet (LFD, 5% fat), high-fat diet (HFD, 15% fat), and berberine-supplemented diet (BSD, 15% fat). The results showed that HFD could decrease the expression of CPT IA and PPARα, while BSD increased those expressions. PMID:26342959

  12. Some remarks on the occurrence, host-specificity and validity of Myxobolus rotundus Nemeczek, 1911 (Myxozoa: Myxosporea).

    Science.gov (United States)

    Molnár, Kálmán; Székely, Csaba; Hallett, Sascha L; Atkinson, Stephen D

    2009-01-01

    Myxobolus rotundus Nemeczek, 1911 is a common and specific parasite of the common bream Abramis brama (L.). Small, round or ellipsoidal shaped plasmodia of this species develop in the gill and exhibit strong histotropism to the secondary gill lamellae with plasmodial development in their capillary network. M. rotundus is frequently found in mixed infection with M. bramae Reuss, 1906, a parasite of the afferent arteries of gill filaments. The round spores of M. rotundus resemble several other Myxobolus spp., but can be distinguished from these by their small subunit ribosomal RNA gene sequence (GenBank accession no. EU710583), which also differs from the sequence for 'M. rotundus' from the skin of Chinese goldfish Carassius auratus auratus (L.), which we suggest has been misidentified. The SSU rRNA gene sequence of M. rotundus myxospores from bream corresponded to Triactinomyxon type 4 actinospores (AY495707) isolated from Tubifex tubifex (Müller) by Hallett et al. (2005), and we infer from this that these are alternate life stages. PMID:19048408

  13. Effect of acid and alkaline solubilization on the properties of surimi based film

    Directory of Open Access Journals (Sweden)

    Thummanoon Prodpran

    2005-05-01

    Full Text Available The effect of acid and alkaline solubilizing processes on the properties of the protein based film from threadfin bream surimi was investigated. Surimi films prepared from both processes had the similar light transmission, tensile strength (TS and elongation at break (EAB (P<0.05. However, film with alkaline process had slightly lower water vapor permeability (WVP, compared to that prepared by acid solubilizing process. The protein concentration in the film-forming solution directly affected the properties of the film. Increase in protein concentration resulted in an increase in TS, EAB as well as WVP. The film prepared by acid solubilizing process had an increase in yellowish color as evidenced by the continuous increase in b* and E* values during the storage at r oom temperature. The acid and alkali solubilizing processes caused the degradation of muscle protein in surimi, especially with increasing exposure time. Therefore, solubilizing process had the influence on the properties of the protein film from threadfin bream surimi.

  14. Nuclear magnetic resonance metabonomic profiling using tO2PLS

    International Nuclear Information System (INIS)

    Graphical abstract: -- Highlights: •Transposition of O2PLS input matrix (tO2PLS) to analyze metabonomics data. •tO2PLS specific components describe features that separate and define sample groups. •Application of tO2PLS to a 1H NMR metabonomics study of black bream fish. -- Abstract: Blood plasma collected from adult fish (black bream, Sparidae) exposed to a dose of 5 mg kg−1 17β-estradiol underwent metabonomic profiling using nuclear magnetic resonance (NMR). An extension of the orthogonal 2 projection to latent structure (O2PLS) analysis, tO2PLS, was proposed and utilized to classify changes between the control and experimental metabolic profiles. As a bidirectional modeling tool, O2PLS examines the (variable) commonality between two different data blocks, and extracts the joint correlations as well as the unique variations present within each data block. tO2PLS is a proposed matrix transposition of O2PLS to allow for commonality between experiments (spectral profiles) to be observed, rather than between sample variables. tO2PLS analysis highlighted two potential biomarkers, trimethylamine-N-oxide (TMAO) and choline, that distinguish between control and 17β-estradiol exposed fish. This study presents an alternative way of examining spectroscopic (metabolite) data, providing a method for the visual assessment of similarities and differences between control and experimental spectral features in large data sets

  15. PCDDs, PCDFs and PCBs in farmed fish produced in Greece: Levels and human population exposure assessment.

    Science.gov (United States)

    Costopoulou, Danae; Vassiliadou, Irene; Leondiadis, Leondios

    2016-03-01

    Fish is among the essential components of Mediterranean diet and has beneficial effects on human health. Farmed fish is an affordable alternative to wild fish and a significant food export product for Greece. Published studies worldwide have reported significant levels of environmental pollutants in fish tissues. Especially for PCDDs/Fs and PCBs, the studies suggest that the most important contribution to human dietary intake is from fish and seafood. In the present study, we investigate the levels of PCDDs/Fs, dioxin-like and non dioxin-like PCBs in the most common farmed fish species produced in Greece i.e. sea bass, sea bream and rainbow trout. These species are widely consumed in Greece and are also exported to many countries worldwide. The mean levels found were WHO-PCDD/F-TEQ: 0.22 pg g(-1) wet weight (w.w.), WHO-PCDD/F-PCB-TEQ: 0.88 pg g(-1) w.w. for sea bream, WHO-PCDD/F-TEQ: 0.13 pg g(-1) w.w., WHO-PCDD/F-PCB-TEQ: 0.68 pg g(-1) w.w. for sea bass and WHO-PCDD/F-TEQ: 0.10 pg g(-1) w.w., WHO-PCDD/F-PCB-TEQ: 0.43 pg g(-1) w.w. for rainbow trout. For non dioxin-like PCBs, mean sum values found were 8.02 ng g(-1) w.w. for sea bream, 5.24 ng g(-1) w.w. for sea bass and 2.90 ng g(-1) w.w. for rainbow trout. All concentrations found were far below maximum levels set by the European Union and in the same range as wild-caught fish also presented for comparison. Daily intake from the consumption of farmed fish species examined is calculated at 1.3 pg WHO-TEQ kg(-1) b.w., which is at the lowest end of TDI values proposed by the WHO. PMID:26745380

  16. Measurement of PCB concentrations in waters using a biomonitoring programme

    International Nuclear Information System (INIS)

    The book describes a PCB biomonitoring programme which was developed for measuring instantaneous PCB concentrations and permits the compilation of PCB action cadastres for different types of waters and subsequent derivation of current trends. Six representative congeners were selected as a basis for the quantitative routine analysis. The fish species bream (abramis brama) and roach (rutilus rutilus) were used as indicators in the PCB biomonitoring programme on account of their distribution and ecological demands. The age and growth rate of each fish destined for analysis was determined so as to ensure that only healthy fish would be used. In both fish species the dorsal musulature with its low scatter of test results and consistent PCB pattern (internal quantification) proved a representative body region. (orig.)

  17. Metazoan parasites of Brama australis from southern Chile: a tool for stock discrimination?

    Science.gov (United States)

    Oliva, M E; Espinola, J F; Ñacari, L A

    2016-03-01

    The metazoan parasites of 403 specimens of the southern ray's bream Brama australis from three localities in southern Chile (Lebu 36° 70' S; 73° 40' W, Calbuco 41° 50' S; 73° 08' W and Punta Arenas 53° 10' S; 70° 50' W) were recorded. More than 23 400 parasite specimens belonging to 12 taxa were registered. Metazoan parasites were dominated by the copepod Hatschekia conifera, constituting 97% of the total number of parasites; the larval cestode Hepatoxylon trichiuri was the second most important parasite (2·1% of the total number of parasites). The remaining 10 species constituted <1% of the metazoan parasites. Parasitological evidence, based on univariate and multivariate analysis, does not support the existence of discrete stocks in the studied zone. PMID:26813161

  18. Evaluation of monitoring data of the German Environmental Specimen Bank using the Hasse diagram technique; Bewertung von Monitoringdaten der Umweltprobenbank des Bundes mit der Hasse-Diagramm-Technik

    Energy Technology Data Exchange (ETDEWEB)

    Helm, D. [Umweltbundesamt, Fachgebiet II 1.4-S, Berlin (Germany)

    2003-07-01

    On behalf of the Federal Environment Ministry the Federal Environmental Agency operates the German Environmental Specimen Bank (ESB), which targets are to record and evaluate pollution data of selected ecological systems. Scope of the present study is to check the possibilities of the Hasse diagram technique (HDT) for the assessment of ESB data, mainly with regard to evaluations and conclusions. Xenobiotic body burden in Bream musculature (Abramis brama); measured in 1997, was exemplary used to answer these questions. Focus was put on ecosystemic assessment. The results clearly show that HDT is a valuable method for the graphical display, interpretation and comparative evaluation of pollution data, HDT visualises data qualitatively and in summary. Additionally, differences between pollution patterns are revealed, which suggest different contamination processes and entries. An ecosystemic approach of assessment is achieved by simultaneous consideration of different xenobiotics. (orig.)

  19. Equilibrium sampling of polychlorinated biphenyls in River Elbe sediments – Linking bioaccumulation in fish to sediment contamination

    DEFF Research Database (Denmark)

    Schäfer, Sabine; Antoni, Catherine; Möhlenkamp, Christel;

    2015-01-01

    sediment (Clip⇔sed) by multiplying concentrations in the equilibrium sampling polymer with lipid to polymer partition coefficients. We have applied silicone coated glass jars for equilibrium sampling of seven ‘indicator’ polychlorinated biphenyls (PCBs) in sediment samples from ten locations along the...... River Elbe to measure Cfree of PCBs and their Clip⇔sed. For three sites, we then related Clip⇔sed to lipid-normalized PCB concentrations (Cbio,lip) that were determined independently by the German Environmental Specimen Bank in common bream, a fish species living in close contact with the sediment: (1......) In all cases, Cbio,lip were below Clip⇔sed, (2) there was proportionality between the two parameters with high R2 values (0.92–1.00) and (3) the slopes of the linear regressions were very similar between the three stations (0.297; 0.327;0.390). These results confirm the close link between PCB...

  20. Use of a wire scanner for monitoring residual gas ionization in Soreq Applied Research Accelerator Facility 20 keV/u proton/deuteron low energy beam transport beam line

    International Nuclear Information System (INIS)

    The ion source end of the Soreq Applied Research Accelerator Facility accelerator consists of a proton/deuteron ECR ion source and a low energy beam transport (LEBT) beam line. An observed reduction of the radio frequency quadrupole transmission with increase of the LEBT current prompted additional study of the LEBT beam properties. Numerous measurements have been made with the LEBT bream profiler wire biased by a variable voltage. Current-voltage characteristics in presence of the proton beam were measured even when the wire was far out of the beam. The current-voltage characteristic in this case strongly resembles an asymmetric diodelike characteristic, which is typical of Langmuir probes monitoring plasma. The measurement of biased wire currents, outside the beam, enables us to estimate the effective charge density in vacuum.

  1. Use of a wire scanner for monitoring residual gas ionization in Soreq Applied Research Accelerator Facility 20 keV∕u proton∕deuteron low energy beam transport beam line.

    Science.gov (United States)

    Vainas, B; Eliyahu, I; Weissman, L; Berkovits, D

    2012-02-01

    The ion source end of the Soreq Applied Research Accelerator Facility accelerator consists of a proton∕deuteron ECR ion source and a low energy beam transport (LEBT) beam line. An observed reduction of the radio frequency quadrupole transmission with increase of the LEBT current prompted additional study of the LEBT beam properties. Numerous measurements have been made with the LEBT bream profiler wire biased by a variable voltage. Current-voltage characteristics in presence of the proton beam were measured even when the wire was far out of the beam. The current-voltage characteristic in this case strongly resembles an asymmetric diodelike characteristic, which is typical of Langmuir probes monitoring plasma. The measurement of biased wire currents, outside the beam, enables us to estimate the effective charge density in vacuum. PMID:22380317

  2. Physicochemical properties and sensory characteristics of sausage formulated with surimi powder.

    Science.gov (United States)

    Santana, Palestina; Huda, Nurul; Yang, Tajul Aris

    2015-03-01

    The objectives of this study were to determine the physicochemical properties and sensory characteristics of fish sausage made with 100 % threadfin bream (Nemipterus japonicus) surimi powder (SP100), a mix of 50 % surimi powder and 50 % frozen surimi (SP50), and a control (100 % frozen surimi). No significant differences in protein content and folding test results (P > 0.05) were detected among the SP100 and SP50 samples and the control. Gel strength of SP100 was lower (P > 0.05) than that of the control. The texture profile analysis (TPA) values (hardness, cohesiveness, springiness, and chewiness) of SP100 were significantly lower (P Malaysian commercial fish sausages. The water holding capacity, and emulsion stability of SP100 were significantly lower (P Malaysian commercial fish sausages. PMID:25745219

  3. Measurement of single-fish target strength in the South China Sea

    Institute of Scientific and Technical Information of China (English)

    CHEN Guobao; LI Yongzhen; CHEN Pimao; ZHANG Jun; FANG Lichen; LI Nana

    2012-01-01

    We measured the target strength (TS) of three commercial fish species:whitespotted spinefoot (Siganus canaliculatus),black porgy (Acanthopagrus schlegelii),and creek red bream (Lutjanus argentimaculatus),in the South China Sea.The TS of caged or tethered fish (n=76 total) was measured using a Simrad EY60 portable scientific echosounder at 120 kHz.We evaluated the relationship between TS and total length (TL,cm) for the three species.This is the first attempt to use split-beam acoustics to measure single-fish TS in the South China Sea by Chinese researchers.Our results will improve the accuracy and precision of acoustic abundance estimates of commercially important species and further the development of underwater acoustic survey techniques in fisheries in the South China Sea.

  4. Recent advances in studies on photosynthetic microorganisms and photobioreactors. ; Production of useful carotenoids using minute algae. Hikari gosei biseibutsu no kino kaihatsu to foto baioriakuta. ; Bisai sorui ni yoru yuyo karotenoido no seisan

    Energy Technology Data Exchange (ETDEWEB)

    Kobayashi, M.; Tsuji, Y. (Higashimaru Shoyu Co. Ltd., Hyogo (Japan)); Kakizono, T.; Nagai, S. (Hiroshima Univ. (Japan). Faculty of Engineering)

    1993-11-25

    Astaxanthin (3,3[prime]-dihydroxy-[beta],[beta]-carotene-4,4[prime]-dione) is a red carotenoid dye present in aquatic organisms such as crustacea and fish, and has a close relationship with the manifestation of colors of the bodies and meat of these organisms. Astaxanthin is currently used as a color-enhancing agent for cultivated fish such as red sea bream, rainbow trout, and salmon. Recently, astaxanthin has been found to be a much stronger antioxidant than [beta]-carotene or [alpha]-tocophenol, and its application to foods and pharmaceuticals is expected. In this article, studies by the authors on Haematococcus pluvialis, one of the most promising microorganisms as a source of producing astaxanthin, are described as an example of production of useful carotenoids using minute algae, and are compared with Dunaliella, as a [beta]-carotene producing microorganism, which is already produced commercially. 1 fig., 1 tab.

  5. Biochemical characteristics of four marine fish skins in Korea.

    Science.gov (United States)

    Cho, Jae-Kwon; Jin, Young-Guk; Rha, Sung-Ju; Kim, Seon-Jae; Hwang, Jae-Ho

    2014-09-15

    In this study, we investigated the biochemical characteristics of the fish skins of four industrial species: olive flounder (Paralichthys olivaceus), black rockfish (Sebastes schlegeli), sea bass (Lateolabrax maculatus) and red sea bream (Pagrus major). There is high domestic demand in Korea for farming of these fish for human consumption. Crude protein contents in the skin of these fish ranged from 73% to 94% by dry weight; this was in part due to a high content of the structural protein, collagen. Among the four species, olive flounder had the thickest dermal and epidermal layers in the dorsal skin. This species was also associated with the highest extraction ratio of acid-soluble collagen. We also examined whether fish skin could be a cost-effective alternative to current fish meal sources. Our analysis indicates that, when supplemented with additional fish oils and essential amino acids, fish skin is a viable alternative for fish meal formulations. PMID:24767045

  6. CHARACTERISTICS AND NEW DATA OF RIVER ILOVA ICHTHYOFAUNA (CENTRAL CROATIA

    Directory of Open Access Journals (Sweden)

    Dušan Jelić

    2009-04-01

    Full Text Available During the research 28 species of freshwater fish were found, belonging to 10 families. Most numerous family was Cyprinidae (18 species, followed by Percidae (3 species and Cobitidae (2 species. Results showed that the most abundant in species were spirlin (Alburnoides bipunctatus (17.4%, common roach (Rutilus rutilus (15.7% and prussian carp (Carassius gibelio (12.8%. And in the terms of total fish weight, highest represented were asp (Aspius aspius (28.9%, bream (Abramis brama (18.7% and pike (Esox lucius (11.4%. Presence of four new fish species has been confirmed in Ilova River: bullhead (Cottus gobio, trout (Salmo trutta, danube whitefinned gudgeon (Romanogobio vladykovi and tench (Tinca tinca.

  7. Local and regional variability in fish community structure, richness and diversity of 56 Danish lakes with contrasting depth and trophic state

    DEFF Research Database (Denmark)

    Menezes, Rosemberg; Borchsenius, Finn; Svenning, J.-C.;

    Habitat distribution of fish might be influenced by food availability, competition, predation,composition of aquatic plants and water clarity. It has been found that a shift from a turbid to a clear water state in a lake lead to higher proportion of piscivorous fish and a habitat shift of prey fish...... determined by a higher risk of predation in the littoral and upper pelagic zones. In Danish lakes, key species such as roach, perch and ruffe have shown a shift towards a more pelagic/profundal distribution in response to reduced nutrient loading, whereas bream and pikeperch remain in the pelagic....../profundal zones. Nevertheless, information about how the within-lake variability in fish abundance, richness and diversity changes in the littoral and pelagic areas along contrasting depth and trophic state is scarce. It is expected that eutrophic lakes present lower within lake habit heterogeneity than...

  8. Isolation of Japanese Encephalitis Virus from Brain Tissues of Bat in Yunnan Province%从云南省蝙蝠脑组织中分离出乙型脑炎病毒

    Institute of Scientific and Technical Information of China (English)

    张海林; 张云智; 黄文丽; 米竹青; 龚鹤琴; 王静林

    2001-01-01

    In July 1997, a strain (GB30) of virus was isolated from 60 samples of brain tissues of Murina aurata (Chiroptera: Vespertilionidae) co llec ted in Gengma county, Yunnan province, China. Isolation of virus was negative fr om 4 samples of brain tissues of Rousettus leschenaulti (Chiroptera: Pteropo did ae) collected in Gengma. GB30 virus strain could regularly cause illness and dea th in suckling mice, produced evident CPE in BHK21 cells. It agglutinated red b lood cells of dove at pH5.75~7.4. This virus has been identified serologically by hemagglutination inhibition and immunofluorescent tests using Japanese enceph alitis (JE), dengue (DEN) type 1,2,3,4, and chikungunya (CHIK) viruses monoclona l antibodies, and JE and sindbis (SIN) viruses immune sera. It showed specific r eaction to JE virus only and no reaction with DEN 1~4, CHIK and SIN viruses. Th erefore it can be identified as JE virus. This is the first report on the isolat ion of JE virus from Murina aurata. The results showed that bats are conside red as the reservoir and amplifier host of JE virus transmission in nature.%为进一步阐明蝙蝠在保存乙脑病毒中的作用,于1997年7月,在云南省耿马 县捕捉蝙蝠64只,取脑组织作病毒分离,从一只金管鼻蝠脑组织中分离出1株病毒。该毒株 能引起BHK21细胞病变和乳鼠发病死亡,在pH5.75~7.4时能凝集鸽红血球,经用单克隆抗 体血凝抑制和免疫荧光试验鉴定,证实为乙型脑炎病毒。进一步证明蝙蝠在乙型脑炎病毒保 存和扩散中具有重要作用。从金管鼻蝠体内分离出乙型脑炎病毒属国内外首次报道。

  9. Fish remnants from the excavations of the Bronze Age barrow near Maryanskoe village (Dnepropetrovsk region, Ukraine

    Directory of Open Access Journals (Sweden)

    O. M. Kovalchuk

    2014-10-01

    Full Text Available The Bronze Age mound (2.5–2.3 kya BC is located near the Maryanskoe village (Apostolovskyi district, Dnepropetrovsk region and was excavated in 1953. The results of determination of the fish remnants, which were found during the excavation, are presented in the paper. Eleven species belonging to 9 genera, 5 families and 5 orders (Acipenseriformes, Cypriniformes, Siluriformes, Esociformes, Perciformes were identified: russian sturgeon Acipenser gueldenstaedtii Brandt et Ratzeburg, 1833, stellate sturgeon A. stellatus Pallas, 1771, common ide Idus idus (Linnaeus, 1758, common roach Rutilus rutilus (Linnaeus, 1758, pontic roach R. frisii (Nordmann, 1840, common bream Abramis brama (Linnaeus, 1758, common carp Cyprinus carpio Linnaeus, 1758, tench Tinca tinca (Linnaeus, 1758, european catfish Silurus glanis Linnaeus, 1758, northern pike Esox lucius (Linnaeus, 1758, and zander Sander lucioperca (Linnaeus, 1758. Most of them are quite common in the Dnieper river basin. It was found that carp fishes predominate in the number of species. Most of the bone remnants in the collection belong to zander, catfish and pike, while common roach, pontic roach and common bream are identified by the few bones. This may indicate a different role of these species in the diet of the local population. The ratio of skeletal elements in the collection is the evidence of the fish cutting on the site. Body length and weight was reconstructed for 64 fish specimens. It was found that they were mature and small-sized, except for catfish, pike and perch. Taking into account the characteristics of the funeral rituals of the Yamna culture population, fish bones from the mound near Maryanskoe can be remnants of the parting meal.

  10. Toxicity of degradation products of the antifouling biocide pyridine triphenylborane to marine organisms.

    Science.gov (United States)

    Onduka, Toshimitsu; Ojima, Daisuke; Ito, Mana; Ito, Katsutoshi; Mochida, Kazuhiko; Fujii, Kazunori

    2013-11-01

    We evaluated the acute toxicities of the main degradation products of pyridine triphenylborane (PTPB), namely, diphenylborane hydroxide (DPB), phenylborane dihydroxide (MPB), phenol, and biphenyl, to the alga Skeletonema costatum, the crustacean Tigriopus japonicus, and two teleosts, the red sea bream Pagrus major and the mummichog Fundulus heteroclitus. DPB was the most toxic of the degradation products to all four organisms. The acute toxicity values of DPB for S. costatum, T. japonicus, red sea bream, and mummichog were 55, 70, 100, and 200-310 μg/L, respectively. The degradation products were less toxic than PTPB to S. costatum and T. japonicus; however, the toxicities of DPB and PTPB to the fish species were similar. We also examined changes in the inhibition of growth rate of S. costatum as well as the percentage of immobilization of T. japonicus as end points of toxicity of PTPB after irradiation of PTPB with 432 ± 45 W/m(2) of 290-700 nm wavelength light. After 7 days of irradiation with this light, the concentration of PTPB in the test solutions decreased markedly. A decrease in toxic effects closely coincided with the decrease in the concentration of PTPB caused by the irradiation. PTPB probably accounted for most of the toxicity in the irradiation test solutions. Because the concentrations of PTPB that were acutely toxic to S. costatum and T. japonicus were <10 % of the corresponding concentrations of its degradation products, PTPB probably accounted for most of the toxicity in the irradiation test solutions. PMID:23929384

  11. Long-term patterns in estuarine fish growth across two climatically divergent regions.

    Science.gov (United States)

    Doubleday, Zoë A; Izzo, Christopher; Haddy, James A; Lyle, Jeremy M; Ye, Qifeng; Gillanders, Bronwyn M

    2015-12-01

    Long-term ecological datasets are vital for investigating how species respond to changes in their environment, yet there is a critical lack of such datasets from aquatic systems. We developed otolith growth 'chronologies' to reconstruct the growth history of a temperate estuarine fish species, black bream (Acanthopagrus butcheri). Chronologies represented two regions in south-east Australia: South Australia, characterised by a relatively warm, dry climate, and Tasmania, characterised by a relatively cool, wet climate. Using a mixed modelling approach, we related inter-annual growth variation to air temperature, rainfall, freshwater inflow (South Australia only), and El Niño-Southern Oscillation events. Otolith chronologies provided a continuous record of growth over a 13- and 21-year period for fish from South Australia and Tasmania, respectively. Even though fish from Tasmania were sourced across multiple estuaries, they showed higher levels of growth synchronicity across years, and greater year-to-year growth variation, than fish from South Australia, which were sourced from a single, large estuary. Growth in Tasmanian fish declined markedly over the time period studied and was negatively correlated to temperature. In contrast, growth in South Australian fish was positively correlated to both temperature and rainfall. The stark contrast between the two regions suggests that Tasmanian black bream populations are more responsive to regional scale environmental variation and may be more vulnerable to global warming. This study highlights the importance of examining species response to climate change at the intra-specific level and further validates the emerging use of growth chronologies for generating long-term ecological data in aquatic systems. PMID:26245148

  12. Maternal inheritance in polyploid fish inferred from mitochondrial ATPase genes analysis

    Institute of Scientific and Technical Information of China (English)

    Jinpeng Yan; Xinhong Guo; Shaojun Liu; Jun Xiao; Zhen Liu; Yubao Chen; Yun Liu

    2009-01-01

    The sequences of the ATPase8/6 genes for the triploid, tetraploid and pentaploid hybrids as well as for their male parent blunt snout bream were determined. In order to examine mitochondrial maternal inheritance, the sequences were subjected to a comparative sequence analysis with the homologous sequences of red crucian carp, their female parent, and zebrafish as the outgroup. Base compo-sition and variation as well as the divergences based on nucleotide sequences and deduced amino acid sequences were calculated. Phy-logenetic trees were also constructed with maximum parsimony (MP), minimum evolution (ME), neighbor joining (NJ) and the unweighted pair group method with arithmetic mean (UPGMA) algorithms in MEGA 3.1. The results showed that most nucleotide sub-stitutions occurred at the third codon position of the two genes and thus represented synonymous mutations. The nucleotide sequence divergences of the ATPase8/6 genes ranged from 0.0% to 21.6% among ingroup samples (three types of polyploids and their parents), and 27.0-28.2% between their ingroup and the outgroup samples. All the polyploids were considerably closer in sequence relationship to the female parent red crucian carp (0.0-3.3%) compared to their male parent blunt snout bream (21.0-21.6%). The phylogenetic trees also showed a similar result. In conclusion, the mitochondrial ATPase8/6 genes of artificial polyploid fish stringently indicated maternal inheritance. Our results also suggested that the ATPase8/6 genes are valuable genetic markers to track genealogies and variations in the progenies of the hybrids.

  13. Ship-based MAX-DOAS measurements of tropospheric NO2 and SO2 in the South China and Sulu Sea

    Science.gov (United States)

    Schreier, S. F.; Peters, E.; Richter, A.; Lampel, J.; Wittrock, F.; Burrows, J. P.

    2015-02-01

    In November 2011, ship-based Multi-Axis Differential Optical Absorption Spectroscopy (MAX-DOAS) measurements were performed within the SHIVA campaign on board RV Sonne in the South China and Sulu Sea. Spectral measurements for a total of eleven days could be used to retrieve tropospheric slant column densities (SCDs) of nitrogen dioxide (NO2) and sulfur dioxide (SO2) in the marine environment. The NO2 fit was performed following recommendations developed during the CINDI campaign and adapted for the ship-based measurements. We found that the inclusion of a cross section for liquid water and an empirical correction spectrum accounting for the effects of liquid water and vibrational Raman scattering (VRS) slightly improved the NO2 fit quality, especially at lower elevation angles and for lower NO2 levels. The conversion of SCDs into tropospheric NO2 vertical columns (TVC NO2) has been achieved using both a simple geometric approach and the Bremian advanced MAX-DOAS Retrieval Algorithm (BREAM), which is based on the optimal estimation method and accounts for atmospheric radiative transfer. We found good agreement between the geometric approach using the 15° measurements and BREAM, revealing that measurements at 15° elevation angle can be used for retrieving TVC NO2 in tropical marine environments when SZA is smaller than 75°. As expected, the values of TVC NO2 were generally low (2 × 1015 molec cm-2) in the morning when the RV Sonne was heading along the coast of Borneo. This is in good agreement with satellite measurements. The results of the profile retrieval show that the boundary layer values of NO2 are environment. Interestingly, we also found elevated tropospheric SO2 amounts for measurements taken in a busy shipping lane, consistent with the time series of tropospheric NO2.

  14. Kudoa iwatai and two novel Kudoa spp., K. trachuri n. sp. and K. thunni n. sp. (Myxosporea: Multivalvulida), from daily consumed marine fish in western Japan.

    Science.gov (United States)

    Matsukane, Yuuki; Sato, Hiroshi; Tanaka, Shuhei; Kamata, Yoichi; Sugita-Konishi, Yoshiko

    2011-04-01

    Infection of marine fish by certain myxosporean species of the genus Kudoa results in unsightly cyst formation in the trunk muscle or post-mortem myoliquefaction, causing a great economic loss to aquaculture industries, capture fisheries, and fish dealers. In addition, consumers encountering unsightly Kudoa cysts in fish fillets believe them to be unknown foreign materials acquired during processing. To identify prevalent Kudoa spp. encountered in daily life by the Japanese population, fresh fish slices (sashimi) or fish fillets with whitish spots were collected during a 7-month period (May to December 2008) at local markets in the city of Yamaguchi, western Japan. Kudoa cysts were found in three Japanese seaperches (Lateolabrax japonicus), two black sea bream (Acanthopagrus schlegelii), two Japanese jack mackerel (Trachurus japonicus), and one albacore (Thunnus alalunga). Kudoa iwatai was identified in all the examined Japanese seaperch and black sea bream from Japan's Inland Sea, as assessed by morphology and genetic analysis of the 18S and 28S ribosomal RNA gene (rDNA). Kudoa trachuri n. sp. from two Japanese jack mackerel fished in the Japanese Sea off Nagasaki and Kudoa thunni n. sp. from one albacore fished in the Pacific Ocean had a spore, which was semiquadrate in shape in apical views and ovoid in lateral views, with four equal shell valves and drop-like polar capsules. Scanning electron microscopy revealed that these three Kudoa species had different types of small projections at the apex of each valve. The 18S and 28S rDNA sequences of K. trachuri n. sp. and K. thunni n. sp. were found to be closely related to those of Kudoa crumena; however, these sequences were distinct in each of the species, which additionally exhibited different morphological features. PMID:21053015

  15. Spatial variation in elemental composition of otoliths of three species of fish (family Sparidae)

    Science.gov (United States)

    Gillanders, B. M.; Kingsford, M. J.

    2003-08-01

    Determining the nursery habitat of fishes that have moved from estuarine nursery habitats is difficult. The elemental fingerprints of otoliths of three species of sparids were determined to investigate their utility as a natural tag of the nursery habitat. Juvenile Pagrus auratus (snapper), Rhabdosargus sarba (tarwhine) and Acanthopagrus australis (bream) were collected from two sites in each of 15, six and three estuaries, respectively, and their otoliths analysed by solution-based inductively coupled plasma-mass spectrometry. Significant differences in otolith chemistry were found for all three species of juveniles collected from different estuaries. The same patterns among estuaries were not seen for all species, although it was not possible to sample the same sites within an estuary for all species. For bream, significant differences in otolith chemistry were found among all three estuaries, whereas for tarwhine the six estuaries were separated into three groups. For snapper, a number of estuaries could be separated, but there was some overlap for other estuaries. All three species were collected from the same site within one estuary and their otoliths analysed. Significant differences were found among species, but the implication of this finding remains unclear as the three species show differences in microhabitat use and may also differ in age. Because the elemental fingerprints of juveniles vary among estuaries or groups of estuaries, the nursery or recruitment estuary of adult fish could now be determined by analysing the juvenile region of adult otoliths. Thus, connectivity between estuaries and open coastal populations could be determined. Such information will have major implications for fisheries management because it will provide information on the distance that fish have moved from their recruitment estuary and the number of estuaries that contribute to each adult population.

  16. NPY and sbGnRH gene expression in juvenile and adult male Brazilian flounder Paralichthys orbignyanus Expressão gênica do NPY e do sbGnRH em machos juvenis e adultos de linguado Paralichthys orbignyanus

    Directory of Open Access Journals (Sweden)

    Vinicius Farias Campos

    2011-11-01

    Full Text Available The objective of this study was to evaluate neuropeptide Y (NPY and sea bream gonadotropin-release hormone (sbGnRH gene expression in juvenile and adult males of Brazilian flounder. Hypothalamuses from fish were sampled for total RNA extraction. After cDNA synthesis, real-time PCR was used to measure gene expression. NPY showed approximately 2-fold increases in their mRNA levels while sbGnRH showed 3-fold increases in adult fish. These results suggest that these peptides could be involved on hypothalamic regulation of Brazilian flounder sexual maturation.O objetivo deste estudo foi avaliar a expressão gênica do neuropeptídeo Y (NPY e da variante sea bream do hormônio liberador de gonadotrofinas (sbGnRH em linguados machos juvenis e adultos. O hipotálamo foi isolado para a extração de RNA total. Após a síntese de cDNA, a PCR em tempo real foi usada para avaliar a expressão gênica. Foi observado um aumento de aproximadamente duas vezes nos níveis de NPY e de aproximadamente três vezes nos níveis de sbGnRH nos peixes adultos. Esses resultados demonstram que estes peptídeos podem estar envolvidos na regulação, via hipotálamo, da maturação sexual no linguado.

  17. ARTIFICIAL PROPAGATION AND BREEDING OF MARINE FISH IN CHINA

    Institute of Scientific and Technical Information of China (English)

    洪万树; 张其永

    2002-01-01

    Since the 1990s, artificial propagation and breeding technique of marine fish in China have developed by way of increasing species and fry numbers, with special stress laid on valuable species. Large quantities of artificial fry can meet the needs of both marine cage culture and pond culture for most species. Experimental results obtained by scientists have been put into use in actual production. Fish fry production has entered a period of sustainable development. So far, at least 44 species (21 families) of marine fish have been successfully bred in China. The artificial fry number of large yellow croaker (Pseudosciaena crocea) exceeded 300 million in 1999. The species whose artificial fry numbers have each surpassed 10 million annually are red drum (Sciaenops ocellatus), Japanese seabass (Lateolabrax japonicus), cuneate drum (Nibea miichthioides), spring spawning red seabream (Pagrosomus major) and threebanded sweetlip (Plectorhynchus cinctus). Millions of artificial fry are bred annually in the species of black porgy (Sparus macrocephalus), Russell's snapper (Lutjanus russelli), javelin grunt (Pomadasys hasta), miiuy croaker (Miichthys miiuy) and skewband grunt (Hapalogenys nitens). The fish in the family Sciaenidae are the main species in artificial propagation and breeding. Some problems and prospects on marine fish culture and stock enhancement are also discussed and some proposals for sustainable development are put forward in this article.

  18. Effects of an artificial reef system on demersal nekton assemblages in Xiangshan Bay, China

    Science.gov (United States)

    Jiang, Yazhou; Lin, Nan; Yuan, Xingwei; Jiao, Haifeng; Shentu, Jikang; Li, Shengfa

    2016-01-01

    In 2012, an artificial reef system was deployed in Xiangshan Bay, China, to enhance its fishery resources. To determine the effect of the artificial reef system on the demersal nekton assemblages, a beforeafter- control-impact study design was applied. Comparisons of assemblages from impact and control habitats revealed that the assemblage in the impact area had a gradual response to reef deployment. The assemblages in the impact and control areas changed in different ways after reef deployment. During the study period, total biomass, species richness and average body weight in the control area remained relatively stable, whereas there were significant increases in these indicators in the impact area. Responses to the reefs differed among nekton species, inducing assemblage succession in the reefs post-deployment. Sparus macrocephalus and Cynoglossus abbreviatus benefited most from reef deployment. Conversely, smallsized shrimp Palaemon gravieri showed a progressive decrease in biomass following reef deployment. Overall, the artificial reef system diversified the demersal nekton assemblage, enhanced the total biomass, and increased the proportion of large-sized species.

  19. The relationships between heavy metal (Cd, Cr, Cu, Fe, Pb, Zn) levels and the size of six Mediterranean fish species

    International Nuclear Information System (INIS)

    Significant relationships between metal concentrations and fish size were negative. - Heavy metal (Cd, Cr, Cu, Fe, Pb, Zn) concentrations in the muscle, gill and liver of six fish species (Sparus auratus, Atherina hepsetus, Mugil cephalus, Trigla cuculus, Sardina pilchardus and Scomberesox saurus) from the northeast Mediterranean Sea were measured and the relationships between fish size (length and weight) and metal concentrations in the tissues were investigated by linear regression analysis. Metal concentrations (as μg/g d.w.) were highest in the liver, except for iron in the gill of Scomberesox saurus and lowest in the muscle of all the fish species. Highest concentrations of Cd (4.50), Cr (17.1) and Pb (41.2) were measured in liver tissues of T. cuculus, Sardina pilchardus and A. hepsetus, respectively. The liver of M. cephalus showed strikingly high Cu concentrations (202.8). The gill of Scomberesox saurus was the only tissue that showed highest (885.5) iron concentrations. Results of linear regression analysis showed that, except in a few cases, significant relationships between metal concentrations and fish size were negative. Highly significant (P<0.001) negative relationships were found between fish length and Cr concentrations in the liver of A. hepsetus and M. cephalus, and Cr concentrations in the gill of T. cuculus. Cr and Pb concentrations in the liver and Cu concentrations in all the tissues of Scomberesox saurus also showed very significant (P<0.001) negative relationships. Negative relationships found here were discussed

  20. Description of Two Species (Including One New Species)of Genus Haliotrema and One Species of Genus Euryhaliotrema%两种海盘虫(包括一新种)及一种宽海盘虫的记述

    Institute of Scientific and Technical Information of China (English)

    李海燕; 颜心茂

    2007-01-01

    记述了寄生于黑鲷Sparus macrocephalus(Basilewsky)与紫红笛鲷Lutjanus argentimaculatus (Forskai)鳃上的海盘虫属-新种及寄生于勒氏笛鲷Lutjanus russelli(Bleek,1849)鳃上的螺管海盘虫Haliotrema spirotubiforum Zhang,2001,长阴宽海盘虫Euryhaliotrema chrysotaeniae(Young,1968)Kritsky&.Boeger,2002.瘤钩海盘虫,新种Haliotrema tukerhamatus sp.nov.的两对中央大钩钩尖基部有一瘤状突起;背中央大钩和背联结片的形状均相似于Haliotrema longihamus Zhukov,1976,但新种背腹中央大钩形状相同,均没有内外突的分化;腹联结片前缘凹入,中部后缘有一显著的向后凸起,而已知种背腹中央大钩形状相异,腹中央大钩内外突分化明显,腹联结片平直;新种交接管近端多次弯曲,远端环绕一圈,而已知种交接管仅在中部环绕一圈.

  1. [Frost-resistance of subtropical evergreen woody plants: an evaluation based on plant functional traits].

    Science.gov (United States)

    Xu, Yi-Lu; Yang, Xiao-Dong; Xu, Yue; Xie, Yi-Ming; Wang, Liang-Yan; Yan, En-Rong

    2012-12-01

    Evaluating the frost-resistance of evergreen woody plants is of significance in guiding the species selection in forest management in subtropical region. In this paper, an investigation was made on the functional traits (including specific leaf area, stem wood density, leaf area, leaf dry matter content, leaf relative electrical conductance, and twig wood density) of 64 common evergreen broad-leaved and coniferous woody plant species in the Ningbo region of Zhejiang Province, East China, after a severe snowstorm in early 2008, aimed to select the evergreen woody plants with high ability of freeze-tolerance, and to establish a related evaluation system. By using a hierarchy analysis approach, the weight values of the functional traits of each species were determined, and an index system for evaluating the plants tolerance ability against freeze and mechanical damage was established. Based on this system, 23 evergreen plant species with high tolerance ability against freeze and mechanical damage, such as Cyclobalanopsis gilva, Cyclobalanopsis nubium, Neolitsea aurata, and Vacciniuim mandarinorum, were selected. In the meantime, on the basis of the ordering with each of the functional traits, the ordering of the tolerance ability of the 64 plant species against freeze and mechanical damage was made, and a list for the frost-resistance ability of the subtropical evergreen woody plant species in Ningbo region was constituted. PMID:23479868

  2. Trace elements in two marine fish species during estuarine residency: Non-essential versus essential

    International Nuclear Information System (INIS)

    Highlights: ► We assessed essential and non-essential trace elements loads in two marine fish. ► We found similarly low levels of Zn, Cr, and As in both sites and species. ► We compared recommended daily allowances with the estimated daily intake. ► Arsenic was higher than tolerable commercial levels and USA average daily intake. - Abstract: Trace element levels in fish are of particular interest, owing the potential risk to human health. In accordance, juveniles of Dicentrarchus labrax and of Liza aurata were sampled and arsenic, cadmium, chromium, selenium and zinc were determined in the muscle. The levels of trace elements in muscle demonstrated to be similar for both species and sites, with the exception of selenium levels at reference, which seemed to be higher in D. labrax. Moreover, apart from arsenic levels in muscle, all elements were in conformity with the existent regulatory guidelines for fish consumption. The dietary intake of each element was also calculated, with arsenic and selenium showing intakes above the recommended dietary allowances. Nevertheless, no arsenic speciation was carried out and thus no accurate risk evaluation could be established. Additionally, selenium levels never exceeded the dietary allowances more than five times, which are considered safe.

  3. The occurrence of acanthocephalans in teleost fish from the Bizerte lagoon, Tunisia.

    Science.gov (United States)

    Gargouri, L; Antar, R; Zarrouk, F; Maamouri, F

    2016-01-01

    Seven species of acanthocephalans were identified for the first time in teleost fish from the Bizerte lagoon, Tunisia, including Neoechinorhynchus (Hebesoma) agilis (Rudolphi, 1819) Van Cleave, 1916; N. (Neoechinorhynchus) rutili Müller, 1780 (Neoechinorhynchidae); Acanthocephaloide propinquus (Dujardin, 1845) Meyer, 1932; A. incrassatus (Molin, 1858) Meyer, 1932 (Arhythmacanthidae); Longicollum pagrosomi Yamaguti, 1935 (Pomphorhynchidae); Rhadinorhynchus cadenati (Golvan et Houin, 1964) Golvan, 1969 (Rhadinorhynchidae); and Telosentis exiguus (von Linstow, 1901) Van Cleave, 1923 (Illiosentidae). Acanthocephaloide propinquus, N. (N.) rutili, L. pagrosomi and R. cadenati were restricted to the rectum and the posterior intestine, whereas the other species (A. incrassatus, T. exiguus and N. (H.) agilis) colonized two or three parts of the intestine. The most diverse acanthocephalan fauna was recorded in Lithognathus mormyrus, with three species; two species were reported from Trachurus trachurus; and a single species was recorded in Liza aurata and in Atherina boyeri. Neoechinorhynchus (N.) rutili, L. pagrosomi and R. cadenati attained the lowest prevalences of less than 6% and were considered as to be rare species. Acanthocephaloide incrassatus, A. propinquus, T. exiguus and N. (H.) agilis were satellite species. The diversity of the acanthocephalan fauna in the fish from the Bizerte lagoon is compared with previous studies from other localities. The report of A. incrassatus, A. propinquus and N. (N.) rutili in L. mormyrus presents new host records in Mediterranean waters. PMID:25726842

  4. Revisión taxonómica del género Pseudocyphellaria Vain. (Lobariaceae-Ascomycetes liquenizados para Colombia

    Directory of Open Access Journals (Sweden)

    Forero Enrique

    2006-06-01

    Full Text Available Se presenta la revisión taxonómica del género Pseudocyphellaria (Ascomycetes liqenizados para Colombia. El género Pseudocyphellaria Vain., con cerca de 110 especies en el mundo, hace parte de la familia Lobariaceae, una
    familia de líquenes de distribución cosmopolita, con mayor número de especies en la región austral. Para Colombia,
    con este estudio, se reconocen seis especies (P. aurata (Ach. Vain., P. crocata (L. Vain., P. intricata (Del. Vain., P. arvidssonii D. Galloway, P. clathrata (De Not. Malme, y P. encoensis R. Sant., de las cuales tres, P. arvidssonii D. Galloway, P. clathrata (De Not. Malme, y P. encoensis R. Sant., constituyen nuevos registros para el país. De igual manera, se presentan descripciones morfoanatómicas, complementadas con datos de pruebas químicas con K, P, C, KC y cromatografías en capa fina de las especies encontradas, así como, comentarios de datos ecológicos y de distribución geográfica. Se incluye una clave genérica ilustrada que permite el fácil reconocimiento del género dentro de la familia Lobariaceae y una clave ilustrada para las especies de Pseudocyphellaria que crecen en Colombia.

  5. Molecular Evolution of Multiple Arylalkylamine N-Acetyltransferase (AANAT in Fish

    Directory of Open Access Journals (Sweden)

    Bina Zilberman-Peled

    2011-05-01

    Full Text Available Arylalkylamine N-acetyltransferase (AANAT catalyzes the transfer of an acetyl group from acetyl coenzyme A (AcCoA to arylalkylamines, including indolethylamines and phenylethylamines. Multiple aanats are present in teleost fish as a result of whole genome and gene duplications. Fish aanat1a and aanat2 paralogs display different patterns of tissue expression and encode proteins with different substrate preference: AANAT1a is expressed in the retina, and acetylates both indolethylamines and phenylethylamines; while AANAT2 is expressed in the pineal gland, and preferentially acetylates indolethylamines. The two enzymes are therefore thought to serve different roles. Here, the molecular changes that led to their specialization were studied by investigating the structure-function relationships of AANATs in the gilthead seabream (sb, Sperus aurata. Acetylation activity of reciprocal mutated enzymes pointed to specific residues that contribute to substrate specificity of the enzymes. Inhibition tests followed by complementary analyses of the predicted three-dimensional models of the enzymes, suggested that both phenylethylamines and indolethylamines bind to the catalytic pocket of both enzymes. These results suggest that substrate selectivity of AANAT1a and AANAT2 is determined by the positioning of the substrate within the catalytic pocket, and its accessibility to catalysis. This illustrates the evolutionary process by which enzymes encoded by duplicated genes acquire different activities and play different biological roles.

  6. The Prevalence and Pathogenicity of the “Anchor Worm” (Lernea spp, Phylum Arthropoda within the Finfish Inhabiting the Danube Delta Area

    Directory of Open Access Journals (Sweden)

    Marius Hangan

    2013-10-01

    Full Text Available This research was conducted as a series of cross-sectional epidemiological studies. It started in the 2003 and ended in 2008, having as sample sites the Sontea-Fortuna, Gorgova-Uzlina, Dunavat-Dranov and Razim-Sinoie lakes. The aim of the research was to assess the distribution and the pathogenicity of the Lernaea copepod among the inhabiting finfish populations, in various seasons of the time period. Lernaea spp was found in ctenopharyngodon idella, cyprinus carpio, liza aurata and liza haematocheila, only in two out of the four sampled complex of lakes, Razim-Sinoie and Dunavat-Dranov. The highest prevalence of the parasite was recorded in the autumns and at the beginning of the springs. The frequency of the parasitism was highest in the C. idella captured in the Dunavat-Dranov complex lakes (86.29%. The lesions caused by the copepod were mainly localized on the eye balls, the tegmentum and on the fins, where hemorrhagic and proliferative processes, as well as an overall increase in tegmentum mucus secretion, were noticed.

  7. ORGANOCHLORINE PESTICIDES IN FISH OF THE KILIYA DELTA OF THE DANUBE (2003–2005

    Directory of Open Access Journals (Sweden)

    Yu. Sytnik

    2015-09-01

    Full Text Available Purpose. Identification and analysis of the content of organochlorine pesticides (OCP in organs and tissues of benthivorous and predatory fish species of the Kiliya delta of the Danube (Eastern mouth and their comparison with previous years. Methodology. The analysis of the samples of aquatic organisms for the content of residual organochlorine pesticides was carried out by gas-liquid chromatography (GLH based on a unified methodology. The chromatographic analysis was carried out on the gas chromatograph "Color 5" with electron capture detector at the Institute of Hydrobiology of the National Academy of Sciences of Ukraine. Findings. The effect of stable organochlorine pesticides on aquatic organisms is characterized by marked persistence in the environment and by high ability for migration and accumulation on different levels of food chains in aquatic ecosystems. The continuous presence of OCP in all investigated organs and tissues of selected species with the presence of a certain amount of fat and consequently, the fixation of residual organochlorine pesticides. Among benthivorous fishes, the content of OCP in organs and tissues (total is as follows: vimba → roach → wild carp → bream → gibel carp. Among the predators: asp → perch → European catfish → pikeperch. Originality. Recently, a significant number of scientists all around the world returns to the problem of the accumulation and distribution (redistribution of OCP in the components of aquatic ecosystems. This is associated with certain threats to the environment and human health, that could lead to negative consequences as a result of the consumption of fish as the highest trophic level in fresh waters of Ukraine. Practical value. The obtained data indicate that the major commercial fish species of the Kiliya delta of the Danube river contains residual quantities of pesticides. This indicates to the need for continuous hygienic and toxicological monitoring of fish caught

  8. (90)Sr in fish from the southern Baltic Sea, coastal lagoons and freshwater lake.

    Science.gov (United States)

    Zalewska, Tamara; Saniewski, Michał; Suplińska, Maria; Rubel, Barbara

    2016-07-01

    Activity concentrations of radioactive (90)Sr were studied in four fish species: herring, flounder, sprat and cod caught in the southern Baltic Sea in two periods: 2005-2009 and 2013-2014. The study included also perch from the coastal lagoons - Vistula Lagoon and Szczcin Lagoon and a freshwater lake - Żarnowieckie Lake as well as additional lake species: pike and bream. (90)Sr activity concentrations were compared in relation to species and to particular tissue: muscle, whole fish (eviscerated) and bones. In 2014, in the Baltic, the maximal (90)Sr concentrations were found in fishbones: herring - 0.39 Bq kg(-1) w.w., cod - 0.48 Bq kg(-1) w.w., and flounder - 0.54 Bq kg(-1) w.w. In the whole fish the maximal concentrations were found in flounder - 0.16 Bq kg(-1) w.w. and cod - 0.15 Bq kg(-1) w.w., while in herring - 0.022 Bq kg(-1) w.w. and sprat - 0.026 Bq kg(-1) w.w. they stayed at lower level. Relatively high (90)Sr concentrations were detected in whole fish from freshwater Lake Żarnowieckie: perch - 0.054 Bq kg(-1) w.w., pike - 0.062 Bq kg(-1) w.w. and bream - 0.140 Bq kg(-1) w.w. Concentration ratio (CR) determined for particular fish tissues and for whole eviscerated fish in relation to (90)Sr concentrations in seawater and lake water were showing significant variability unlike the corresponding (137)Cs concentration ratios which are stable and specific for fish species. The study corroborates with the conviction of the growing role of (90)Sr in the overall radioactivity in the southern Baltic Sea as compared to (137)Cs. PMID:27061778

  9. Variations of 210Po and 210Pb in various marine organisms from Western English Channel: contribution of 210Po to the radiation dose

    International Nuclear Information System (INIS)

    Measurements of 210Po were carried out in various marine matrices (mussels, oysters, seaweed, fish, and abalones) and in seawater at several points along the French coast, over a period of 2 years (2003-2005). These measurements contribute to a better knowledge of this element, since few recent data exist for the French coast. Marked seasonal variations have been revealed in some species and there are differences according to the way of life of these species. Activities in mussels (Mytilus edulis) and oysters (Crassostrea gigas) are similar and varying between 90 and 600 Bq kg-1 (d.w.). Activities in macroalgae (Fucus serratus) are lowest, between 4 and 16 Bq kg-1 (d.w.). In oyster, abalone (Haliotis tuberculata) and fish (Solea solea, Sparus sp.), the strongest activities are measured in the digestive glands, the gills and the gonads. 210Po/210Pb ratios in all cases have values of more than one for all species. From a significant number of measurements, CFs were calculated for seaweed (between 4.6 x 103 and 5.0 x 103) and for molluscs, with highest CFs (>105) found for the digestive gland and gills of the oysters, the digestive gland of the abalones and the liver of fish. Finally, the activities measured have made it possible to estimate the internal dose from chronic exposure due to 210Po received by the marine organisms (0.05 μG h-1 for macroalgae, between 0.70 and 1.5 μG h-1 for mussels and oyster), and the contribution of seafood to the dose received by humans (46-129 μSv y-1)

  10. Variations of {sup 210}Po and {sup 210}Pb in various marine organisms from Western English Channel: contribution of {sup 210}Po to the radiation dose

    Energy Technology Data Exchange (ETDEWEB)

    Connan, O. [Institut de Radioprotection et de Surete Nucleaire, Laboratoire de Radioecologie de Cherbourg-Octeville, IRSN/DEI/SECRE/LRC, Rue Max Pol Fouchet, 50130 Cherbourg-Octeville (France)], E-mail: olivier.connan@irsn.fr; Germain, P.; Solier, L.; Gouret, G. [Institut de Radioprotection et de Surete Nucleaire, Laboratoire de Radioecologie de Cherbourg-Octeville, IRSN/DEI/SECRE/LRC, Rue Max Pol Fouchet, 50130 Cherbourg-Octeville (France)

    2007-10-15

    Measurements of {sup 210}Po were carried out in various marine matrices (mussels, oysters, seaweed, fish, and abalones) and in seawater at several points along the French coast, over a period of 2 years (2003-2005). These measurements contribute to a better knowledge of this element, since few recent data exist for the French coast. Marked seasonal variations have been revealed in some species and there are differences according to the way of life of these species. Activities in mussels (Mytilus edulis) and oysters (Crassostrea gigas) are similar and varying between 90 and 600 Bq kg{sup -1} (d.w.). Activities in macroalgae (Fucus serratus) are lowest, between 4 and 16 Bq kg{sup -1} (d.w.). In oyster, abalone (Haliotis tuberculata) and fish (Solea solea, Sparus sp.), the strongest activities are measured in the digestive glands, the gills and the gonads. {sup 210}Po/{sup 210}Pb ratios in all cases have values of more than one for all species. From a significant number of measurements, CFs were calculated for seaweed (between 4.6 x 10{sup 3} and 5.0 x 10{sup 3}) and for molluscs, with highest CFs (>10{sup 5}) found for the digestive gland and gills of the oysters, the digestive gland of the abalones and the liver of fish. Finally, the activities measured have made it possible to estimate the internal dose from chronic exposure due to {sup 210}Po received by the marine organisms (0.05 {mu}G h{sup -1} for macroalgae, between 0.70 and 1.5 {mu}G h{sup -1} for mussels and oyster), and the contribution of seafood to the dose received by humans (46-129 {mu}Sv y{sup -1})

  11. Histological and molecular studies of species of Myxobolus Bütschli, 1882 (Myxozoa: Myxosporea) in the gills of Abramis, Blicca and Vimba spp. (Cyprinidae), with the redescription of M. macrocapsularis Reuss, 1906 and M. bliccae Donec & Tozyyakova, 1984.

    Science.gov (United States)

    Molnár, Kálmán; Cech, Gábor; Székely, Csaba

    2011-06-01

    Although Myxobolus spp. from cyprinid fishes are generally characterised by a strict host-specificity, this study has found that the breams Abramis brama (L.), Blicca bjoerkna (L.) and Vimba vimba (L.) may be infected by the same Myxobolus spp. It is demonstrated that M. macrocapsularis Reuss, 1906, a parasite of the gill filaments of B. bjoerkna, can also infect A. brama. In the same way, M. bliccae Donec & Tozyyakova, 1984, also a parasite of B. bjoerkna, can also occur in V. vimba. The molecular sequences of M. macrocapsularis spores from B. bjoerkna and A. brama were 100% identical. Two of the 18S rDNA sequences of three replicate samples of M. bliccae from B. bjoerkna were 100% identical, whereas the third sequence exhibited a 99.7% similarity with sequences from V. vimba. M. bliccae sequences of spores collected from V. vimba showed a 99.8% similarity to the first two isolates and 99.6% to the third. Data obtained by morphological, histological and molecular biological methods all suggest that Myxobolus spp., known for their strict host-specificity, may sometimes infect several closely related cyprinids. PMID:21544710

  12. Distribution of metals (Fe, Mn, Zn, Cu) in fish tissues in two lakes of different trophy in Northwestern Poland.

    Science.gov (United States)

    Rajkowska, Monika; Protasowicki, Mikołaj

    2013-04-01

    This study presents concentrations of iron, manganese, zinc, and copper in selected tissues of two fish species: pike (Esox lucius L.) and bream (Abramis brama L.) living in lakes Ińsko and Wisola, Northwestern Poland. The lakes differ in their trophic status. The effect of gender and environmental conditions on metals accumulation was also investigated. Metal analyses were performed using inductively coupled plasma atomic emission spectroscopy. Considering all studied fish species and tissues, the average metal concentrations (micrograms per gram wet weight) in both lakes occurred in the following ranges: Fe 0.8-240.6, Mn 0.2-8.4, Zn 3.0-185.9, and Cu 0.14-7.76. The lowest levels of the studied metals were always detected in the muscles. The spleen, kidneys, and liver were found to accumulate the highest amounts of Fe. In the case of the other metals, the highest levels were found, as follows: Mn in skin, gills, and gonads, Zn in digestive tract and gills, Cu in liver. Heavy metal content in fish gonads was observed to be sex dependent. PMID:22923375

  13. Reservoir to river passage of age-0+ year fishes, indication of a dispersion pathway for a non-native species.

    Science.gov (United States)

    Janáč, M; Jurajda, P; Kružíková, L; Roche, K; Prášek, V

    2013-03-01

    This study demonstrates passage of age-0+ year individuals of pikeperch Sander lucioperca, common bream Abramis brama and non-native tubenose goby Proterorhinus semilunaris from the Nové Mlýny Reservoir into the River Dyje (Danube River basin, Czech Republic) through the turbine of a hydropower facility. Most fishes had standard length (LS ) in the range 12-33 mm. Seasonal patterns corresponded with spawning activity, i.e. an early single spawning event for S. lucioperca, multiple spawning events for A. brama and continuous spawning with a later start and prolonged duration for P. semilunaris. Sander lucioperca, P. semilunaris and larger A. brama (>22 mm) drifted almost exclusively during the dark; smaller A. brama displayed no preference for light or dark. Proterorhinus semilunaris displayed significantly lower mortality than other species when passing through the turbine (3% compared to 18%). The passage of high numbers of P. semilunaris from the reservoir (estimated at 473 000 individuals per year), and their subsequent mass downstream drift, may have contributed to rapid population establishment along the River Dyje and the quick downstream expansion. PMID:23464556

  14. Distribution and Diet of 0+ Fish within a Canyon-Shaped European Reservoir in Late Summer

    Science.gov (United States)

    Vaek, Mojmír; Kubeka, Jan; Matna, Josef; Sea, Jaromír

    2006-05-01

    The distribution and diet of age 0+ fish were studied in the deep canyon-shaped ímov Reservoir (Czech Republic), which is characterized by a longitudinal trophic gradient. During late summer of two years, 0+ fish were sampled from inshore and offshore habitats along the longitudinal reservoir axis. Offshore catches of 0+ fish from the surface layer were dominated by roach (Rutilus rutilus ), bream (Abramis brama ) and perch (Perca fluviatilis ), whereas in the deeper open water perch predominated. Inshore catches of 0+ fish were constituted mainly by perch and roach. The proportions of roach in the inshore catches were highest at the upper and most eutrophic part of the reservoir, whereas the proportions of perch in the inshore catches were higher at the downstream areas. Total catches of both inshore and offshore 0+ fish increased upstream in the reservoir. Offshore 0+ perch were of consistently smaller size than inshore 0+ perch. Inshore 0+ perch had significantly smaller size at the upstream reservoir part than at the downstream, more lacustrine regions. The diet of both inshore and offshore 0+ fish consisted predominantly of crustacean zooplankton. Perch diet was generally dominated both by cladocerans and copepods, whereas roach diet consisted chiefly of cladocerans.

  15. Histopathology of feral fish from a PCB-contaminated freshwater lake

    Energy Technology Data Exchange (ETDEWEB)

    Koponen, Kari; Ritola, Ossi; Huuskonen, Sirpa E.; Lindstroem-Seppae, Pirjo [Univ. of Kuopio (Finland). Dept. of Physiology; Myers, Mark S. [National Oceanic and Atmospheric Administration, Seattle, WA (United States). National Marine Fisheries Service

    2001-05-01

    The purpose of this study was to evaluate the potential toxic effects of chronic sublethal polychlorinated biphenyl (PCB) exposure on feral fish, using histopathology as an endpoint. Histopathological study of bream (Abramis brama) and asp (Aspius aspius) living in a PCB-polluted freshwater lake revealed abnormal cellular changes in the renal corpuscle of both species. Dilation of glomerular capillaries (DGC), mesangial edema (ME), an adhesion between visceral and parietal layers of Bowman's capsule (ABC), and filling of Bowman's space (FBS), were highly prevalent features in lake fish. The prevalence of each of these lesions was significantly lower, or totally absent in fish caught from reference locations. Cellular alterations in liver, gill, gonads, spleen, and intestine were all linked to seasonal changes. The results suggest that some of the observed histopathological changes in renal glomeruli, particularly DGC and ME, could possibly indicate a prolonged chemical stress caused by PCBs and related compounds. It is also possible that chronic PCB exposure may have suppressed and weakened the immuno systems of exposed fish making them more vulnerable to secondary parasitic infection.

  16. Biomonitoring of polychlorinated biphenyls (PCBs) in heavily polluted aquatic environment in different fish species.

    Science.gov (United States)

    Brázová, Tímea; Hanzelová, Vladimíra; Miklisová, Dana; Šalgovičová, Danka; Turčeková, L'udmila

    2012-11-01

    The distribution and concentrations of polychlorinated biphenyls (PCBs) were determined in fish species (European perch Perca fluviatilis, northern pike Esox lucius, pike perch Sander lucioperca, wels catfish Silirus glanus, common carp Cyprinus carpio, European eel Anguilla anguilla, freshwater bream Abramis brama, goldfish Carassius auratus, and roach Rutilus rutilus) in a heavily polluted water reservoir Zemplínska šírava (Slovakia). The study performed at two different time points 5 years apart (2004 and 2009) revealed serious PCB contamination of fish muscle tissue and significant interspecies as well as tissue-specific differences in PCB uptake by fish. Total PCBs broadly correlated with the trophic position of individual fish species within a food chain (P 0.05). The study has shown that the kind of fish, its feeding habit, and specific conditions of the habitat are mutually interrelated factors that are responsible for significant variations in fish body burdens. A tendency to PCB biomagnification was also proved in some fish species of this water reservoir. PMID:22173787

  17. Sizing up your enemy: individual predation vulnerability predicts migratory probability.

    Science.gov (United States)

    Skov, Christian; Baktoft, Henrik; Brodersen, Jakob; Brönmark, Christer; Chapman, Ben B; Hansson, Lars-Anders; Nilsson, P Anders

    2011-05-01

    Partial migration, in which a fraction of a population migrate and the rest remain resident, occurs in an extensive range of species and can have powerful ecological consequences. The question of what drives differences in individual migratory tendency is a contentious one. It has been shown that the timing of partial migration is based upon a trade-off between seasonal fluctuations in predation risk and growth potential. Phenotypic variation in either individual predation risk or growth potential should thus mediate the strength of the trade-off and ultimately predict patterns of partial migration at the individual level (i.e. which individuals migrate and which remain resident). We provide cross-population empirical support for the importance of one component of this model--individual predation risk--in predicting partial migration in wild populations of bream Abramis brama, a freshwater fish. Smaller, high-risk individuals migrate with a higher probability than larger, low-risk individuals, and we suggest that predation risk maintains size-dependent partial migration in this system. PMID:20980300

  18. Determination of sulfur and selected trace elements in metallothionein-like proteins using capillary electrophoresis hyphenated to inductively coupled plasma mass spectrometry with an octopole reaction cell

    International Nuclear Information System (INIS)

    The determination of sulfur in biologically relevant samples such as metalloproteins is described. The analytical methodology used is based on robust on-line coupling between capillary electrophoresis (CE) and octopole reaction cell inductively-coupled plasma mass spectrometry (ORC-ICP-MS). Polyatomic ions that form in the plasma and interfere with the determination of S at mass 32 are minimised by addition of xenon to the collision cell. The method has been applied to the separation and simultaneous element-specific detection of sulfur, cadmium, copper, and zinc in commercially available metallothionein preparations (MT) and metallothionein-like proteins (MLP) extracted from liver samples of bream (Abramis brama L.) caught in the river Elbe, Germany. Instrumental detection limits have been calculated according to the German standard procedure DIN 32645 for the determination of sulfur and some simultaneously measured trace elements in aqueous solution. For sulfur detection limits down to 1.3 μg L-1 (34S) and 3.2 μg L-1 (32S) were derived. For the other trace elements determined simultaneously detection limits ranging from 300 ng L-1 (58Ni) to 500 ng L-1 (66Zn, 55Mn) were achieved. For quantification of sulfur and cadmium in a commercially available MT preparation under hyphenated conditions the use of external calibration is suggested. Finally, the need for proper sample-preparation technique will be discussed. (orig.)

  19. [Differences in the infestation rate of young cyprinid fishes (Cypriniformes) by metacercaria of Posthodiplostomum Cuticola (Digenea, Diplostomatidae) in river and lake systems of the Lake Chany basin (Western Siberia)].

    Science.gov (United States)

    Iadrenkina, E N

    2014-01-01

    A total of 12 fish species were studied for the invasion of P. cuticola (Nordmann, 1832) metacercariae in the Chany Lake estuaries, river and lake systems during different periods of water level. All infected individuals were represented by juveniles of the family Cyprinidae, except for adults of the dace Leuciscus leuciscus. (L.). Under an average water level (2010) the highest rate of fish invasion was revealed in the estuary zone, where the prevalence of infection (PI) constituted 37.5%, 13.4%, and 5.9% for the gudgeon Gobio gobio, the roach Rutilus rutilus (L.), and the dace L. leuciscus (L.), respectively. An infested carp Cyprinus carpio L. (Heckel) (PI - 13.5%) was recorded in the lower Kargat River, and the goldfish Carassius auratus (L.) (PI - 3.2%), in the lake system. Mean intensity of infection (MI) constituted 1.3, 1.4, 2.5, 2.6, and 1.0 in gudgeon, roach, dace, carp, and bream (Abramis brama L.), respectively. During dry season (2011) with high water salinity only two fish species were infected with P. cuticola metacercariae, the goldfish (PI - 7.9%) and the roach (PI - 1.5%). No correlation between PI and fish density was revealed. It is assumed that the high degree of water salinity is a limiting factor regulating the efficiency of cercariae transition from the first intermediate host (Planorbis planorbis (L.)) to the second one (Pisces, Cyprinidae). PMID:25693328

  20. Endocrine disrupting chemicals-Linking internal exposure to vitellogenin levels and ovotestis in Abramis brama from Dutch surface waters.

    Science.gov (United States)

    Reinen, Jelle; Suter, Marc J-F; Vögeli, A Christiane; Fernandez, Mariana F; Kiviranta, Hannu; Eggen, Rik I L; Vermeulen, Nico P E

    2010-11-01

    The exposure of male bream from three Dutch freshwater locations to endocrine disrupting compounds (EDCs) and corresponding effects are described in this study. Fish specimen displaying reproductive disorders associated with high levels of plasma vitellogenin (VTG) concentrations and occurrence of ovotestis (OT) were investigated. To provide information on the full spectrum of EDCs in fish tissue, adipose tissue samples of individual fish were analyzed for nearly 130 chemicals targeting different compound classes (bisphenols, alkylphenols, pesticides, polychlorinated dibenzo-p-dioxins (PCDDs), dibenzofurans (PCDFs), polychlorinated biphenyls (PCBs), hydroxylated polychlorinated biphenyls (OH-PCBs), polybrominated diphenyl ethers (PBDEs) and biphenyls (PBBs)) and steroid hormones. To establish whether tissue from specimen with reproductive disorders shows a spectrum of EDCs that is qualitatively and quantitatively different from that of controls free of symptoms, bioassay-directed fractionation was performed using the recombinant yeast estrogen screen (YES), the E-Screen bioassay, the human sulfotransferase 1E1 (SULT1E1) inhibition assay, and the coumestrol-based estrogen receptor α (ERα) high resolution screening (HRS) assay. No differences in estrogenicity could be observed between the cases and controls and steroidal estrogens accounted for the majority of estrogenicity found in the complex mixtures. In this study, the combination of the different assays employed to measure total estrogenicity and the SULT1E1 inhibition does not predict the outcome of unwanted physiological effects, however, it can be used to determine the presence of EDCs in fish samples and their estrogenic effects. PMID:21787654