WorldWideScience

Sample records for brazil genotype distribution

  1. Geographic distribution of hepatitis C virus genotypes in Brazil

    Directory of Open Access Journals (Sweden)

    Campiotto S.

    2005-01-01

    Full Text Available Brazil is a country of continental dimension with a population of different ethnic backgrounds. Thus, a wide variation in the frequencies of hepatitis C virus (HCV genotypes is expected to occur. To address this point, 1,688 sequential samples from chronic HCV patients were analyzed. HCV-RNA was amplified by the RT-PCR from blood samples collected from 1995 to 2000 at different laboratories located in different cities from all Brazilian States. Samples were collected in tubes containing a gel separator, centrifuged in the site of collection and sent by express mail in a refrigerated container to Laboratório Bioquímico Jardim Paulista, São Paulo, SP, Brazil. HCV- RNA was extracted from serum and submitted to RT and nested PCR using standard procedures. Nested PCR products were submitted to cycle sequencing reactions without prior purification. Sequences were analyzed for genotype determination and the following frequencies were found: 64.9% (1,095 for genotype 1, 4.6% (78 for genotype 2, 30.2% (510 for genotype 3, 0.2% (3 for genotype 4, and 0.1% (2 for genotype 5. The frequencies of HCV genotypes were statistically different among Brazilian regions (P = 0.00017. In all regions, genotype 1 was the most frequent (51.7 to 74.1%, reaching the highest value in the North; genotype 2 was more prevalent in the Center-West region (11.4%, especially in Mato Grosso State (25.8%, while genotype 3 was more common in the South (43.2%. Genotypes 4 and 5 were rarely found and only in the Southeast, in São Paulo State. The present data indicate the need for careful epidemiological surveys throughout Brazil since knowing the frequency and distribution of the genotypes would provide key information for understanding the spread of HCV.

  2. Hepatitis B virus genotypes circulating in Brazil: molecular characterization of genotype F isolates

    Directory of Open Access Journals (Sweden)

    Virgolino Helaine A

    2007-11-01

    Full Text Available Abstract Background Hepatitis B virus (HBV isolates have been classified in eight genotypes, A to H, which exhibit distinct geographical distributions. Genotypes A, D and F are predominant in Brazil, a country formed by a miscegenated population, where the proportion of individuals from Caucasian, Amerindian and African origins varies by region. Genotype F, which is the most divergent, is considered indigenous to the Americas. A systematic molecular characterization of HBV isolates from different parts of the world would be invaluable in establishing HBV evolutionary origins and dispersion patterns. A large-scale study is needed to map the region-by-region distribution of the HBV genotypes in Brazil. Results Genotyping by PCR-RFLP of 303 HBV isolates from HBsAg-positive blood donors showed that at least two of the three genotypes, A, D, and F, co-circulate in each of the five geographic regions of Brazil. No other genotypes were identified. Overall, genotype A was most prevalent (48.5%, and most of these isolates were classified as subgenotype A1 (138/153; 90.2%. Genotype D was the most common genotype in the South (84.2% and Central (47.6% regions. The prevalence of genotype F was low (13% countrywide. Nucleotide sequencing of the S gene and a phylogenetic analysis of 32 HBV genotype F isolates showed that a great majority (28/32; 87.5% belonged to subgenotype F2, cluster II. The deduced serotype of 31 of 32 F isolates was adw4. The remaining isolate showed a leucine-to-isoleucine substitution at position 127. Conclusion The presence of genotypes A, D and F, and the absence of other genotypes in a large cohort of HBV infected individuals may reflect the ethnic origins of the Brazilian population. The high prevalence of isolates from subgenotype A1 (of African origin indicates that the African influx during the colonial slavery period had a major impact on the circulation of HBV genotype A currently found in Brazil. Although most genotype F

  3. Biotyping and genotyping (MLVA16 of Brucella abortus isolated from cattle in Brazil, 1977 to 2008.

    Directory of Open Access Journals (Sweden)

    Sílvia Minharro

    Full Text Available Brucellosis is a worldwide distributed zoonosis that causes important economic losses to animal production. In Brazil, information on the distribution of biovars and genotypes of Brucella spp. is scarce or unavailable. This study aimed (i to biotype and genotype 137 Brazilian cattle isolates (from 1977 to 2008 of B. abortus and (ii to analyze their distribution. B. abortus biovars 1, 2 and 3 (subgroup 3b were confirmed and biovars 4 and 6 were first described in Brazil. Genotyping by the panel 1 revealed two groups, one clustering around genotype 40 and another around genotype 28. Panels 2A and 2B disclosed a high diversity among Brazilian B. abortus strains. Eighty-nine genotypes were found by MLVA16. MLVA16 panel 1 and 2 showed geographic clustering of some genotypes. Biotyping and MLVA16 genotyping of Brazilian B. abortus isolates were useful to better understand the epidemiology of bovine brucellosis in the region.

  4. Prevalence, incidence density, and genotype distribution of GB virus C infection in a cohort of recently HIV-1-infected subjects in Sao Paulo, Brazil.

    Directory of Open Access Journals (Sweden)

    Maria Teresa M Giret

    Full Text Available BACKGROUND: The results of previous studies elsewhere have indicated that GB virus C (GBV-C infection is frequent in patients infected with the human immunodeficiency virus type 1 (HIV-1 due to similar transmission routes of both viruses. The aim of this study was to determine the prevalence, incidence density and genotypic characteristics of GBV-C in this population. METHODOLOGY/PRINCIPAL FINDINGS: The study population included 233 patients from a cohort primarily comprised of homosexual men recently infected with HIV-1 in São Paulo, Brazil. The presence of GBV-C RNA was determined in plasma samples by reverse transcriptase-nested polymerase chain reaction and quantified by real-time PCR. GBV-C genotypes were determined by direct sequencing. HIV viral load, CD4+ T lymphocyte and CD8+ T lymphocyte count were also tested in all patients. The overall prevalence of GBV-C infection was 0.23 (95% CI: 0.18 to 0.29 in the study group. There was no significant difference between patients with and without GBV-C infection and Glycoprotein E2 antibody presence regarding age, sex, HIV-1 viral load, CD4+ and CD8+T cell counts and treatment with antiretroviral drugs. An inverse correlation was observed between GBV-C and HIV-1 loads at enrollment and after one year. Also, a positive but not significant correlation was observed between GBV-C load and CD4+ T lymphocyte. Phylogenetic analysis of the GBV-C isolates revealed the presence of genotype 1 and genotype 2, these sub classified into subtype 2a and 2b. CONCLUSION/SIGNIFICANCE: GBV-C infection is common in recently HIV -1 infected patients in Sao Paulo, Brazil and the predominant genotype is 2b. This study provides the first report of the GBV-C prevalence at the time of diagnosis of HIV-1 and the incidence density of GBV-C infection in one year.

  5. Asian genotypes of dengue virus 4 in Brazil.

    Science.gov (United States)

    Pinho, A C O; Sardi, S I; Paula, F L; Peixoto, I B; Brandão, C J; Fernandez, F M C; Campos, G S

    2015-10-01

    Dengue virus, commonly transmitted by mosquitoes, causes a human disease of significant social impact and presents a serious public health problem in Brazil. This report describes the unusual emergence of DENV-4 in northern Brazil after a nearly 30-year-long absence. DENV-4 genotype I is of Asian origin and was identified in the serum of patients receiving treatment at a hospital serving the Salvador area (Brazilian state of Bahia). The identification of dengue virus serotypes through molecular and phylogenetic analysis is essential for predicting disease severity or fatal illness, principally in endemic countries such as Brazil.

  6. HIV AND HCV COINFECTION: PREVALENCE, ASSOCIATED FACTORS AND GENOTYPE CHARACTERIZATION IN THE MIDWEST REGION OF BRAZIL

    Directory of Open Access Journals (Sweden)

    Solange Zacalusni Freitas

    2014-12-01

    Full Text Available A cross-sectional study on prevalence, associated factors and genotype distribution of HCV infection was conducted among 848 HIV-infected patients recruited at reference centers in the Midwest Region of Brazil. The prevalence rate of HIV-HCV coinfection was 6.9% (95% CI: 5.2 to 8.6. In multivariable analysis, increasing age, use of illicit drugs (injection and non-injection, a history of blood transfusion before 1994, and the absence of a steady partnership were significant independent associated factors for HIV-HCV coinfection. The phylogenetic analysis based on the NS5B region revealed the presence of two major circulating genotypes of HCV: genotypes 1 (58.3% and 3 (41.7%. The prevalence of HIV-HCV coinfection was lower than those reported in studies conducted with HIV-infected patients in different regions of Brazil, due to the fact that illicit drug use is not a frequent mode of HIV transmission in this region of Brazil. Serologic screening of HIV-patients for HCV before initiating antiretroviral treatment, a comprehensive identification of associated factors, and the implementation of effective harm reduction programs are highly recommended to provide useful information for treatment and to prevent HCV coinfection in these patients.

  7. HIV and HCV coinfection: prevalence, associated factors and genotype characterization in the Midwest Region of Brazil.

    Science.gov (United States)

    Freitas, Solange Zacalusni; Teles, Sheila Araújo; Lorenzo, Paulo Cesar; Puga, Marco Antonio Moreira; Tanaka, Tayana Serpa Ortiz; Thomaz, Danilo Yamamoto; Martins, Regina Maria Bringel; Druzian, Angelita Fernandes; Lindenberg, Andréa Siqueira Campos; Torres, Marina Sawada; Pereira, Sérgio A; Villar, Livia Melo; Lampe, Elisabete; Motta-Castro, Ana Rita Coimbra

    2014-01-01

    A cross-sectional study on prevalence, associated factors and genotype distribution of HCV infection was conducted among 848 HIV-infected patients recruited at reference centers in the Midwest Region of Brazil. The prevalence rate of HIV-HCV coinfection was 6.9% (95% CI: 5.2 to 8.6). In multivariable analysis, increasing age, use of illicit drugs (injection and non-injection), a history of blood transfusion before 1994, and the absence of a steady partnership were significant independent associated factors for HIV-HCV coinfection. The phylogenetic analysis based on the NS5B region revealed the presence of two major circulating genotypes of HCV: genotypes 1 (58.3%) and 3 (41.7%). The prevalence of HIV-HCV coinfection was lower than those reported in studies conducted with HIV-infected patients in different regions of Brazil, due to the fact that illicit drug use is not a frequent mode of HIV transmission in this region of Brazil. Serologic screening of HIV-patients for HCV before initiating antiretroviral treatment, a comprehensive identification of associated factors, and the implementation of effective harm reduction programs are highly recommended to provide useful information for treatment and to prevent HCV coinfection in these patients.

  8. Estimation of Genotype Distributions and Posterior Genotype Probabilities for β-Mannosidosis in Salers Cattle

    OpenAIRE

    Taylor, J F; Abbitt, B.; Walter, J P; Davis, S. K.; Jaques, J. T.; Ochoa, R. F.

    1993-01-01

    β-Mannosidosis is a lethal lysosomal storage disease inherited as an autosomal recessive in man, cattle and goats. Laboratory assay data of plasma β-mannosidase activity represent a mixture of homozygous normal and carrier genotype distributions in a proportion determined by genotype frequency. A maximum likelihood approach employing data transformations for each genotype distribution and assuming a diallelic model of inheritance is described. Estimates of the transformation and genotype dist...

  9. Genotypic characterization of psittacid herpesvirus isolates from Brazil

    Science.gov (United States)

    Luppi, Marcela Miranda; Luiz, Ana Paula Moreira Franco; Coelho, Fabiana Magalhães; Ecco, Roselene; da Fonseca, Flávio Guimarães; Resende, Mauricio

    2016-01-01

    Thirty-six isolates of psittacid herpesvirus (PsHV), obtained from 12 different species of psittacids in Brazil, were genotypically characterized by restriction fragment length polymorphism (RFLP) analysis and PCR amplification. RFLP analysis with the PstI enzyme revealed four distinct restriction patterns (A1, X, W and Y), of which only A1 (corresponding to PsHV-1) had previously been described. To study PCR amplification patterns, six pairs of primers were used. Using this method, six variants were identified, of which, variants 10, 8, and 9 (in this order) were most prevalent, followed by variants 1, 4, and 5. It was not possible to correlate the PCR and RFLP patterns. Twenty-nine of the 36 isolates were shown to contain a 419 bp fragment of the UL16 gene, displaying high similarity to the PsHV-1 sequences available in GenBank. Comparison of the results with the literature data suggests that the 36 Brazilian isolates from this study belong to genotype 1 and serotype 1. PMID:26887248

  10. RAPD identification of Varroa destructor genotypes in Brazil and other regions of the Americas.

    Science.gov (United States)

    Guerra, J C V; Issa, M R C; Carneiro, F E; Strapazzon, R; Moretto, G

    2010-01-01

    The mite Varroa destructor is the main pest causing damage to apiculture worldwide. In Brazil and other parts of the world, where bees of African origin and their hybrids predominate, the bees can survive these mites without treatment. Studies have shown a correlation between the various genotypes of the mite and its fertility in different geographical regions. Information about mite genotype could be helpful in understanding the diverse effects and relationships of the mite with bees in different regions of the world. DNA analysis by RAPD technique has permitted identification of three distinct genotypes in the mite V. destructor, namely Russian, Japanese and Papua New Guinea. We found predominance of the Russian genotype in Brazil, along with other parts of South America, and in Cuba and Mexico. The Japanese genotype was exclusively found on Fernando de Noronha Island in Brazil.

  11. Dengue virus 3 genotype I in Aedes aegypti mosquitoes and eggs, Brazil, 2005-2006.

    Science.gov (United States)

    Vilela, Ana P P; Figueiredo, Leandra B; dos Santos, João R; Eiras, Alvaro E; Bonjardim, Cláudio A; Ferreira, Paulo C P; Kroon, Erna G

    2010-06-01

    Dengue virus type 3 genotype I was detected in Brazil during epidemics in 2002-2004. To confirm this finding, we identified this virus genotype in naturally infected field-caught Aedes aegypti mosquitoes and eggs. Results showed usefulness of virus investigations in vectors as a component of active epidemiologic surveillance. PMID:20507754

  12. An autochthonous case of hepatitis C virus genotype 5a in Brazil: phylogenetic analysis

    DEFF Research Database (Denmark)

    Ribeiro, L.C.; Souto, F.J.D.; do Espirito-Santo, M.P.;

    2009-01-01

    strain is not closely related to any sequences of genotype 5a from other geographical areas, diverging from the African and European subclades known so far. These data suggest that genotype 5a HCV might have been circulating at a low level in Brazil longer than previously supposed....

  13. Atypical Toxoplasma gondii genotype in feral cats from the Fernando de Noronha Island, northeastern Brazil.

    Science.gov (United States)

    Melo, R P B; Almeida, J C; Lima, D C V; Pedrosa, C M; Magalhães, F J R; Alcântara, A M; Barros, L D; Vieira, R F C; Garcia, J L; Mota, R A

    2016-07-15

    Toxoplasma gondii isolates from Brazil have a different phenotypic and genotypic pattern, with predominance of virulent isolates and recombinant genotypes, compared to the North Hemisphere. Considering that a new T. gondii genotype, non-pathogenic to mice, was previously identified from free-range chickens from the Fernando de Noronha Island, Brazil, this study aimed to identify genotypes of this parasite in tissue samples of feral cats (Felis catus) from this Brazilian Island. Anti-T. gondii IgG antibodies were detected in 18/31 (58%) feral cats. Two non-virulent T. gondii isolates were obtained by mouse bioassay. Genotyping was performed by PCR-RFLP using 10 genetic markers (SAG1, SAG2, SAG3, BTUB, GRA6, c22-8, c29-2, PK1, L358 and Apico) and an atypical strain of T. gondii (ToxoDB #146) was identified. This is the first report of this genotype in feral cats. PMID:27270396

  14. Prevalence of hepatitis C virus (HCV infection and HCV genotypes of hemodialysis patients in Salvador, Northeastern Brazil

    Directory of Open Access Journals (Sweden)

    Silva L.K.

    2006-01-01

    Full Text Available Hepatitis C virus (HCV infection has been identified as the major cause of chronic liver disease among patients on chronic hemodialysis (HD, despite the important reduction in risks obtained by testing candidate blood donors for anti-HCV antibodies and the use of recombinant erythropoietin to treat anemia. A cross-sectional study was performed to estimate the prevalence of HCV infection and genotypes among HD patients in Salvador, Northeastern Brazil. Anti-HCV seroprevalence was determined by ELISA in 1243 HD patients from all ten different dialysis centers of the city. HCV infection was confirmed by RT-PCR and genotyping was performed by restriction fragment length polymorphism. Anti-HCV seroprevalence among HD patients was 10.5% (95% CI: 8.8-12.3 (Murex anti-HCV, Abbott Murex, Chicago, IL, USA. Blood samples for qualitative HCV detection and genotyping were collected from 125/130 seropositive HD patients (96.2%. HCV-RNA was detected in 92/125 (73.6% of the anti-HCV-positive patients. HCV genotype 1 (77.9% was the most prevalent, followed by genotype 3 (10.5% and genotype 2 (4.6%. Mixed infections of genotypes 1 and 3 were found in 7.0% of the total number of patients. The present results indicate a significant decrease in anti-HCV prevalence from 23.8% detected in a study carried out in 1994 to 10.5% in the present study. The HCV genotype distribution was closely similar to that observed in other hemodialysis populations in Brazil, in local candidate blood donors and in other groups at risk of transfusion-transmitted infection.

  15. Global distribution of novel rhinovirus genotype

    DEFF Research Database (Denmark)

    Briese, Thomas; Renwick, Neil; Venter, Marietjie;

    2008-01-01

    Global surveillance for a novel rhinovirus genotype indicated its association with community outbreaks and pediatric respiratory disease in Africa, Asia, Australia, Europe, and North America. Molecular dating indicates that these viruses have been circulating for at least 250 years....

  16. Global distribution of novel rhinovirus genotype

    DEFF Research Database (Denmark)

    Briese, Thomas; Renwick, Neil; Venter, Marietjie;

    2008-01-01

    Global surveillance for a novel rhinovirus genotype indicated its association with community outbreaks and pediatric respiratory disease in Africa, Asia, Australia, Europe, and North America. Molecular dating indicates that these viruses have been circulating for at least 250 years Udgivelsesdato...

  17. Risk Factors for Infection with Different Hepatitis C Virus Genotypes in Southern Brazil

    Directory of Open Access Journals (Sweden)

    Marisa Lúcia Romani Paraboni

    2012-01-01

    Full Text Available Objectives. To investigate the proportion of different genotypes in countryside microregions in southern Brazil, and their association with risk factors. Methods. Cross-sectional study including a convenience sample of patients who tested positive for HCV-RNA and were referred to a regional health center for genotyping, from December 2003 to January 2008. Data were obtained through the National Disease Surveillance Data System, from laboratory registers and from patient charts. Identification of genotypes was carried out using the Restriction Fragment Length Polymorphism “in house” technique. Independent associations with genotypes were evaluated in multinomial logistic regression and prevalence rates of genotypes were estimated with modified Poisson regression. Results. The sample consisted of 441 individuals, 41.1±12.0 years old, 56.5% men. Genotype 1 was observed in 41.5% (95% CI 37.9–48.1 of patients, genotype 2 in 19.3% (95% CI 15.0–23.6, and genotype 3 in 39.2% (95% CI 35.6–43.0. HCV genotype was significantly associated with gender and age. Dental procedures were associated with higher proportion of genotype 2 independently of age, education, and patient treatment center. Conclusions. The hepatitis C virus genotype 1 was the most frequent. Genotype 2 was associated with female gender, age, and dental procedure exposition.

  18. Distribution of HCV genotypes in the metropolitan area of Naples

    Directory of Open Access Journals (Sweden)

    Giuseppe Sodano

    2013-08-01

    Full Text Available The Hepatitis C virus is characterized by high genomic variability that leads to the identification of six different genotypes and many subtypes. In this work, we show the prevalence of genotypes in patients living in the metropolitan area of Naples collected in one year, analyzing differences in the distribution depending on sex and age groups.

  19. Toxoplasma gondii in free-ranging wild small felids from Brazil: molecular detection and genotypic characterization.

    Science.gov (United States)

    Cañón-Franco, W A; Araújo, F A P; López-Orozco, N; Jardim, M M A; Keid, L B; Dalla-Rosa, C; Cabral, A D; Pena, H F J; Gennari, S M

    2013-11-01

    Brazil harbors the largest number of wild Neotropical felid species, with ten of the twelve species recorded in the American continent. Although these animals are considered to be definitive hosts for Toxoplasma gondii, there are few descriptions of the parasite in these species. Here, we performed a molecular detection of T. gondii by amplification of the marker ITS-1 from tissue samples obtained from 90 free-ranging wild small Neotropical felids from Rio Grande do Sul - Brazil. Of the sampled animals, 34.4% (n=31) were positive including the species Puma yagouaroundi - jaguarondi (9/22), Leopardus geoffroyi - Geoffroy's cat (6/22), Leopardus tigrinus - oncilla (8/28), Leopardus wiedii - margay (6/10), Leopardus pardalis - ocelot (1/1) and Leopardus colocolo - Pampas cat (1/7). Toxoplasma DNA was detected with a frequency of 14.6% (63/433) in primary samples of tongue (16/56), brain (8/43), skeletal muscle (15/83), heart (7/63), diaphragm (3/56), vitreous humor (2/44), eye muscle (6/44) and eyeball (6/44). Multilocus PCR-RFLP genotyping of eleven small Neotropical felids using the molecular markers SAG1, 5'3'SAG2, alt. SAG2, SAG3, BTUB, GRA6, c22-8, c29-2, L358, PK1, Apico and CS3 allowed the partial characterization of eight genotypes. We fully characterized two new genotypes that have not been described previously in Brazil (Lw#31Tn from L. wiedii and Py#21Sm from P. yagouaroundi) and one genotype Py#56Br from P. yagouaroundi that has been described previously in isolates from cats, dogs and capybaras from São Paulo state. This study constitutes the first detection and genotypic characterization of T. gondii in free-ranging felids in Brazil, demonstrating the occurrence of the parasite in wild populations and suggesting its potential transmissibility to humans and other domestic and wild animals. PMID:23932730

  20. Hepatitis B Virus Genotype D Isolates Circulating in Chapeco, Southern Brazil, Originate from Italy.

    Directory of Open Access Journals (Sweden)

    Carolina Souza Gusatti

    Full Text Available Hepatitis B virus genotype A1 (HBV/A1, of African origin, is the most prevalent genotype in Brazil, while HBV/F predominates in the other South American countries. However, HBV/D is the most common in the three states of southern Brazil, where 'islands' of elevated prevalence, as Chapecó and other cities, have been described. In this study, 202 HBV chronic carriers attending in 2013 the viral hepatitis ambulatory of Chapecó, were investigated. In comparison with previous studies performed in the same ambulatory, a rapid aging of the HBV infected population was observed (mean age of the newly diagnosed patients increasing from 29.9 ± 10.3 years in 1996 to 44.4 ± 13.3 years in 2013, probably due to a singular vaccination schedule at Chapecó that included not only children but also adolescents. Phylogenetic and BLAST analyses (S region classified 91 HBV isolates into genotypes A (n = 3 and D (n = 88. The majority of HBV/D isolates were closely related to D3 sequences. To understand the reasons for the absence or near absence of genotypes A and F, and how HBV/D was introduced in the south of Brazil, HBV/D infected patients were inquired about their genealogical and geographical origins. Forty-three (52% patients have their four grandparents of Italian origin, vs. seven (8% who have their four grandparents of Brazilian origin. At all, 65 out of 83 (78% patients had at least one grandparent originating from Italy. Taking into consideration the fact that Italy is one of the few countries where subgenotype D3 is predominant, the results strongly suggested that HBV/D was introduced in Brazil through Italian immigration which culminated between 1870 and 1920.

  1. Distribution of Gossypol Glands on Cotton (Gossypium hirsutum L. Genotypes

    Directory of Open Access Journals (Sweden)

    Yuksel BOLEK

    2010-06-01

    Full Text Available Knowing the distribution of gossypol glands in cotton will help to improve advanced cultivars with great economic return. The aim is to compare cotton genotypes for the number and the distribution of gossypol glands found in different plant parts and to determine the genotypes which contain more gossypol glands in their vegetative parts but little or none in their seeds. The intensity and the greatness of the gossypol amount is an important part of defending against plant insects and diseases. Randomized complete block design with two replications was applied at the Agricultural Research Institute of Kahramanmara? during 2006-2007. Thirty seven cotton genotypes belonging to Gossypium hirsutum L. species were the plant material. The number of gossypol glands on stem, boll walls (carpels, leaves and seeds were visually counted and ranged from 0 to 142 per cm2 for stem, 0 to 135 per cm2 for leaf, 0 to 85 per cm2 for boll wall and 0 to 16 per mm2 for embryo (kernel. Number of gossypol glands varied for the genotypes and years but this variance was too low in some genotypes. Genotypes �Acala Royale�, �Acala Prema�, �Deltaopal� and �Carmen� had relatively more gossypol glands on their vegetative parts but less in their seed. Measured parameters were positively and significantly correlated to each other. Correlations for number of gossypol glands between stem and leaf (r=0.77**, boll wall (r=0.65** and seed (r=0.32** were significant.

  2. HIV-1 genotypic resistance profile of patients failing antiretroviral therapy in Paraná, Brazil

    Directory of Open Access Journals (Sweden)

    Paula Virginia Michelon Toledo

    2010-08-01

    Full Text Available Antiretroviral therapy (ART has reduced morbidity and mortality related to human immunodeficiency virus (HIV infection, but in spite of this advance, HIV mutations decrease antiretroviral susceptibility, thus contributing to treatment failure in patients. Genotyping HIV-1 allows the selection of new drugs after initial drug failure. This study evaluated the genotypic profile of HIV-1 isolates from treated (drug-experienced patients in Paraná, Brazil. The prevalence of mutations in reverse transcriptase (RT and protease (PR genes were assessed. We analyzed 467 genotypes of patients with HIV-1 viral loads above 1,000 copies/mL. Mutations at HIV-1 RT and PR genes and previously used ART regimens were recorded. The most prevalent RT mutations were: 184V (68.31%, 215YF (51.6%, 103NS (46%, 41L (39.4%, 67N (38.54%, 210W (23.5%, 190ASE (23.2%, and 181C (17.4%. PR mutations were 90M (33.33%, 82ATFS (29%, 46I (26.8% and 54V (22.2%. The prevalence of mutations was in line with previous national and international reports, except to nonnucleoside analogue reverse transcriptase inhibitors related mutations, which were more prevalent in this study. Previous exposure to antiretroviral drugs was associated with genotypic resistance to specific drugs, leading to treatment failure in HIV patients.

  3. A Sign of Superspreading in Tuberculosis Highly Skewed Distribution of Genotypic Cluster Sizes

    NARCIS (Netherlands)

    Ypma, Rolf J. F.; Altes, Hester Korthals; van Soolingen, Dick; Wallinga, Jacco; van Ballegooijen, W. Marijn

    2013-01-01

    Background: Molecular typing is a valuable tool for gaining insight into spread of Mycobacterium tuberculosis. Typing allows for clustering of cases whose isolates share an identical genotype, revealing epidemiologic relatedness. Observed distributions of genotypic cluster sizes of tuberculosis (TB)

  4. A sign of superspreading in tuberculosis: highly skewed distribution of genotypic cluster sizes.

    NARCIS (Netherlands)

    Ypma, R.J.; Altes, H.K.; Soolingen, D. van; Wallinga, J.; Ballegooijen, W.M. van

    2013-01-01

    BACKGROUND: Molecular typing is a valuable tool for gaining insight into spread of Mycobacterium tuberculosis. Typing allows for clustering of cases whose isolates share an identical genotype, revealing epidemiologic relatedness. Observed distributions of genotypic cluster sizes of tuberculosis (TB)

  5. Distribution study of Chlamydia trachomatis genotypes in symptomatic patients in Buenos Aires, Argentina: association between genotype E and neonatal conjunctivitis

    Directory of Open Access Journals (Sweden)

    Corominas Ana I

    2010-02-01

    Full Text Available Abstract Background Chlamydia trachomatis infections are the most prevalent sexually transmitted bacterial infections in the world. There is scarce data available referring to the distribution of C. trachomatis genotypes in Argentina. The aim of this study was to identify the genotypes of C. trachomatis circulating in the metropolitan area of Buenos Aires (Argentina associated with ophthalmia neonatorum and genital infections. Findings From 2001 to 2006, 199 positive samples for C. trachomatis infection from symptomatic adult patients and neonates with ophthalmia neonatorum from two public hospitals were studied. C. trachomatis genotypes were determined by PCR-RFLP of an ompA fragment. Genotype E was the most prevalent regardless of the sample origin (46.3% 57/123 in adults and 72.4% 55/76 in neonates, followed by genotype D (19.5% 24/123 and F (14.6% 18/123 in adults, and G (9.2% 7/76 and D (7.9% 6/76 in neonates. We detected a significantly higher frequency of genotype E (p ophthalmia neonatorum than in genital specimens. Genotype D was associated with genital localization (p Conclusion We found a particularly increased frequency of C. trachomatis genotype E in neonatal conjunctivitis, which may indicate an epidemiological association between this genotype and the newborn population. The present study also contributed to increase the knowledge on genotype distribution of Chlamydia trachomatis in symptomatic adult patients in Buenos Aires, Argentina, in which genotypes E, D and F were the predominant ones.

  6. Infection of the central nervous system with dengue virus 3 genotype I causing neurological manifestations in Brazil

    Directory of Open Access Journals (Sweden)

    Danilo Bretas de Oliveira

    2016-02-01

    Full Text Available Abstract: A case of dengue virus 3 (DENV-3 genotype I infection with neurological manifestations occurred in Belo Horizonte, Minas Gerais in October 2012. The serotype was detected by PCR, and the genotype was assessed by sequencing and phylogenetic analysis of the C-prM region. The virus causing neurological manifestations clustered with other sequences of DENV-3 genotype I. Because neurological manifestations of DENV are possibly misdiagnosed in Brazil, this study serves as an alert of the importance of DENV diagnoses in CNS infections.

  7. Mycorrhizal colonization and diversity and corn genotype yield in soils of the Cerrado region, Brazil

    Directory of Open Access Journals (Sweden)

    Sueli da Silva Aquino

    2015-12-01

    Full Text Available The degree of interaction between arbuscular mycorrhizal fungi (AMF and host plants appears to depend on plant genotype, which differentially promotes symbiosis and leads to different degree of mycotrophism. The aim of the present study was to assess root colonization, spore density, and diversity of AMF, as well as yield of corn (Zea mays genotypes in soil from the Cerrado region of Brazil. Number of spores (NS, mycorrhizal colonization (COL, plant and ear height, dry weight (DW, and grain yield (GY were assessed in inbred lines and their hybrids. Randomized blocks were used for the experimental design, with 30 treatments (genotypes and three repetitions. The experiment was conducted on the farm of the UNESP-São Paulo State University, Campus of Ilha Solteira, and the plots were composed of two 2-m-long rows, with 0.85 m between rows, and 0.20 m between plants. Qualitative and quantitative changes were observed in fungal compositions, as well as preferential associations between symbiotic partners. Analysis of AMF diversity revealed the presence of 12 species, with Scutellospora calospora and Entrophospora colombiana being the most abundant in all treatments. The species Acaulospora longula, Glomus etunicatum, Glomus macrocarpum, and Gigaspora margarita exhibited preferential associations with certain genotypes. COL and GY differed among inbred lines and single-cross hybrids and the significant correlations between COL and the DW and GY showed positive interactions between the plants and AMF. There was no correlation between heterosis for GY and heterosis for colonization. These single-cross hybrids HS83 × E3 and D3 × F5 exhibited high GY, evidencing a productivity potential for the low technological condition used.

  8. Hepatitis B virus genotype E detected in Brazil in an African patient who is a frequent traveler

    Directory of Open Access Journals (Sweden)

    R Sitnik

    2007-12-01

    Full Text Available Genotype E of hepatitis B virus (HBV has not been described in Brazil and is found mainly in Africa. Genotype A is the most prevalent in Brazil, and genotypes B, C, D, and F have already been reported. We report here an HBV genotype E-infected patient and some characterization of surface (S protein, DNA polymerase (P and precore/core (preC/C coding regions based on the viral genome. The patient is a 31-year-old black man with chronic hepatitis B who was born and raised in Angola. He has been followed by a hepatologist in São Paulo, Brazil, since November 2003, and he is a frequent traveler to Latin America, Africa, and Europe. In 2003, he was diagnosed with HBV infection and started treatment with lamivudine with the later addition of adefovir dipivoxil. No known risk factor was identified. Serologically, he is HBsAg and anti-HBe positive, but HBeAg and anti-HBs negative. DNA sequence analysis of the S/P region confirmed that this patient is infected with genotype E, subtype ayw4. The preC/C region showed G1896A and G1899A mutations but no mutations in the basal core promoter. Nucleotide substitutions common in genotype E were also observed (C1772, T1858 and A1757. Although this is not an autochthonous case and there is no evidence of further spread, the description of this case in Brazil highlights the current risk of viral genotypes spreading with unprecedented speed due to constant travel around the world.

  9. Genotyping and antimicrobial susceptibility of Clostridium perfringens isolated from Tinamidae, Cracidae and Ramphastidae species in Brazil

    Directory of Open Access Journals (Sweden)

    Rodrigo Otávio Silveira Silva

    2014-03-01

    Full Text Available The aim of this study was to isolate, genotype and evaluate the antimicrobial susceptibility of Clostridium perfringens found in species Tinamidae, Cracidae and Ramphastidae in Brazil. C. perfringens was isolated in 13 (5% out of 260 swab samples and five (8.3% out of 60 stool samples. All strains were classified as C. perfringens type A, and nine (50% were positive for the beta-2 toxin-encoding gene. No strains were positive for the necrotic enteritis toxin B-like (NetB-encoding gene. All isolates were susceptible to penicillin, metronidazole and vancomycin, whereas four (22.2%, five (27.8% and 13 (72.2% strains were considered resistant to erythromycin, oxytetracycline and lincomycin, respectively.

  10. Diversity of genotypes in CTX-M-producing Klebsiella pneumoniae isolated in different hospitals in Brazil

    Directory of Open Access Journals (Sweden)

    Thiago Pavoni Gomes Chagas

    2011-10-01

    Full Text Available OBJECTIVE: The present study was undertaken to characterize CTX-M ESBL-producing Klebsiella pneumoniae collected from hospitals in different cities of Brazil. MATERIAL AND METHODS: Eighty-five K. pneumoniae strains isolated from hospitalized patients in six different hospitals of three cities of Brazil were analyzed. ESBL production was confirmed by the standard double-disk synergy test and the Etest®. The MIC50 and MIC90 for ESBL-producing isolates were determined by the Etest® method. The antimicrobial susceptibilities of bacterial isolates were determined using the agar diffusion method according to the CLSI. Screening for blaTEM, blaSHV, blaCTX-M genes and class 1 integron was performed by PCR amplification. To determine the genomic diversity of CTX-M-producers, isolates were analyzed by macrorestriction profile analysis following PFGE. RESULTS AND DISCUSSION: Seventy-one K. pneumoniae isolates were ESBL-producing. PCR and sequencing experiments detected 38 CTX-M-producing K. pneumoniae belonged to groups CTX-M 1, CTX-M 2, CTX-M 8 and CTX-M 9. The association of different types ESBL (CTX-M, SHV and TEM was frequent. All K. pneumoniae isolates carried class 1 integron. PFGE analysis revealed thirty-one clonal types among CTX-M-producing isolates. The data presented herein illustrate the diversity of genotypes of CTX-M producing K. pneumoniae among Brazilians hospitals.

  11. Genotypic and antimicrobial characterization of pathogenic bacteria at different stages of cattle slaughtering in southern Brazil.

    Science.gov (United States)

    Loiko, Márcia R; de Paula, Cheila M D; Langone, Ana C J; Rodrigues, Rochele Q; Cibulski, Samuel; Rodrigues, Rogério de O; Camargo, Anderson C; Nero, Luís A; Mayer, Fabiana Q; Tondo, Eduardo C

    2016-06-01

    Meat can be contaminated in different stages of the slaughtering process and the identification of these stages is the starting point to implement adequate control measures. The objectives of this study were to assess the presence of pathogenic microorganisms in cattle carcasses, to identify the most important contamination points of the slaughtering process, and to evaluate the possible risk factors related to them in a cattle slaughterhouse. To this aim, 108 cattle carcasses were sampled at three stages of the slaughtering process: Point 1 (hides after bleeding); Point 2 (carcasses after hide removal); and Point 3 (carcasses immediately after division). Escherichia coli O157:H7, Listeria monocytogenes and Salmonella Livingstone were isolated from the carcasses. Phenotypic and genotypic characterization indicated that there was cross-contamination among animals, since bacteria with identical genotypic and phenotypic profiles were isolated from different animals at the same sampling day. Furthermore, this is the first report about the isolation of E. coli O157:H7 in a bovine slaughterhouse from southern Brazil. PMID:26896744

  12. Global geographic distribution of Trichinella species and genotypes.

    Science.gov (United States)

    Feidas, Haralambos; Kouam, Marc K; Kantzoura, Vaia; Theodoropoulos, Georgios

    2014-08-01

    Maximum entropy ecological niche modeling was utilized to describe the global geographic distribution of Trichinella species and genotypes and to assess their invasive risk in new areas other than the ones currently known. Also, space-time scan statistic was utilized to identify global spatiotemporal clusters of infection. A database containing 3209 records for 12 species and genotypes identified at the International Trichinella Reference Center (ITRC) as well as climate, elevation, and land cover data extracted from various databases were used. Ecological niche modeling implemented in the Maxent program indicated new potential ranges for T. spiralis (T1), T. nativa (T2), T. britovi (T3), T. pseudospiralis (T4), T. murrelli (T5), T6, T. papuae (T10), and T. zimbabwensis (T11). The area under the curve values for the test data of the models ranged from 0.901 to 0.998, indicating that the models were very good to excellent. The most important bioclimatic factor in modeling the ranges for T. spiralis (T1), T. nativa (T2), T. britovi (T3), T6, and T. zimbabwensis (T11) was temperature, for T. pseudospiralis (T4) and T. papuae (T10) was precipitation, and for T. murrelli (T5) was land cover. T. spiralis (T1), T. britovi (T3), and T. pseudospiralis (T4) had the same primary land cover which was "Grass Crops". The primary land covers were "Conifer Boreal Forest" for T. nativa (T2), "Cool Fields and Woods" for T. murrelli (T5), "Upland Tundra" for T6, "Tropical Rainforest" for T. papuae (T10), and "Crops and Town" for T. zimbabwensis (T11). The scan statistic analyses revealed the presence of significant spatiotemporal clusters (ppseudospiralis (T4), T. murrelli (T5), T6, and T. nelsoni (T7). No significant clusters were found for T. papuae (T10) and T. zimbabwensis (T11). PMID:24951834

  13. Prevalence, genotypes and factors associated with HCV infection among prisoners in Northeastern Brazil

    Institute of Scientific and Technical Information of China (English)

    Bruno Fernandes de Oliveira Santos; Nathalie Oliveira de Santana; Alex Vianey Callado Franca

    2011-01-01

    AIM: To determine hepatitis C virus (HCV) seroprevalence and its genotypes, and to identify the factors associated with HCV infection. METHODS: This cross-sectional study, conducted in two prisons (one male and one female) in the State of Sergipe, Brazil, comprised 422 subjects. All of the prisoners underwent a rapid test for the detection of HCV antibodies. Patients with a positive result were tested for anti- HCV by enzyme linked immunosorbent assay and for HCV RNA by qualitative polymerase chain reaction (PCR). The virus genotype was defined in every serum sample that presented positive for PCR-HCV. In order to determine the factors independently associated with positive serology for HCV, multivariate logistic regression was used. RESULTS: HCV seroprevalence was 3.1%. Of the 13 subjects with positive anti-HCV, 11 had viremia confirmed by PCR. Of these, 90.9% had genotype 1. A total of 43 (10.2%) were injecting drug users, and HCV seroprevalence in this subgroup was 20.6%. The variable most strongly associated with positive serology for HCV was use of injecting drugs [odds ratio (OR), 23.3; 95% confidence interval (CI), 6.0-90.8]. Age over 30 years (OR, 5.5; 95%CI, 1.1-29.2), history of syphilis (OR, 9.8; 95%CI, 1.7-55.2) and history of household contact with HCV positive individual (OR, 14.1; 95%CI, 2.3-85.4) were also independently associated with HCV infection. CONCLUSION: Most of the HCV transmissions result from parenteral exposure. However, there is evidence to suggest a role for sex and household contact with an infected subject in virus transmission.

  14. Potential geographic distribution of hantavirus reservoirs in Brazil.

    Directory of Open Access Journals (Sweden)

    Stefan Vilges de Oliveira

    Full Text Available Hantavirus cardiopulmonary syndrome is an emerging zoonosis in Brazil. Human infections occur via inhalation of aerosolized viral particles from excreta of infected wild rodents. Necromys lasiurus and Oligoryzomys nigripes appear to be the main reservoirs of hantavirus in the Atlantic Forest and Cerrado biomes. We estimated and compared ecological niches of the two rodent species, and analyzed environmental factors influencing their occurrence, to understand the geography of hantavirus transmission. N. lasiurus showed a wide potential distribution in Brazil, in the Cerrado, Caatinga, and Atlantic Forest biomes. Highest climate suitability for O. nigripes was observed along the Brazilian Atlantic coast. Maximum temperature in the warmest months and annual precipitation were the variables that most influence the distributions of N. lasiurus and O. nigripes, respectively. Models based on occurrences of infected rodents estimated a broader area of risk for hantavirus transmission in southeastern and southern Brazil, coinciding with the distribution of human cases of hantavirus cardiopulmonary syndrome. We found no demonstrable environmental differences among occurrence sites for the rodents and for human cases of hantavirus. However, areas of northern and northeastern Brazil are also apparently suitable for the two species, without broad coincidence with human cases. Modeling of niches and distributions of rodent reservoirs indicates potential for transmission of hantavirus across virtually all of Brazil outside the Amazon Basin.

  15. High proportion of hepatitis C virus genotypes 1 and 3 in a large cohort of patients from Southern Brazil

    Directory of Open Access Journals (Sweden)

    Cláudia Maria Dornelles da Silva

    2007-11-01

    Full Text Available Hepatitis C virus (HCV isolates have been divided into six genotypes (1 to 6. The duration of hepatitis C standard treatment is 48 weeks for patients infected with HCV genotype 1 vs 24 weeks for those infected with genotypes 2 and 3. A total of 1544 HCV isolates from chronic patients living in the southern Brazilian states of Rio Grande do Sul (RS, n = 627 and Santa Catarina (SC, n = 917 were genotyped by restriction fragment length polymorphism (RFLP of polymerase chain reaction (PCR products. In RS, 338 (53.9%; 95% CI 50.0 - 57.8%, 34 (5.4%; 95% CI 3.8 - 7.4% and, 255 (40.7%; 95% CI 36.9 - 44.6% samples were from genotypes 1, 2, and 3, respectively. In SC, 468 (51%; 95% CI 47.8 - 54.2%, 26 (2.9%; 95% CI 1.9 - 4.1% and, 423 (46.1%; 95% CI 42.9 - 49.3% samples were from genotypes 1, 2, and 3, respectively. Genotyping results were confirmed by direct nucleotide sequencing of PCR products derived from 68 samples, without any discrepancy between PCR-RFLP and nucleotide sequencing methods. In conclusion, almost half of the hepatitis C patients from South of Brazil are infected by genotypes 2 and 3 and, these results have important consequential therapeutic implications as they can be treated for only 24 weeks, not 48.

  16. Detection of the emerging rotavirus G12P[8] genotype at high frequency in brazil in 2014: Successive replacement of predominant strains after vaccine introduction.

    Science.gov (United States)

    Luchs, Adriana; Cilli, Audrey; Morillo, Simone Guadagnucci; Gregório, Debora de Souza; de Souza, Karen Aparecida Farias; Vieira, Heloísa Rosa; Fernandes, Adeline de Mira; Carmona, Rita de Cássia Compagnoli; Timenetsky, Maria do Carmo Sampaio Tavares

    2016-04-01

    The continuum characterization of rotavirus (RVA) genotypes is essential to understand how vaccine introduction could impact virus epidemiology. In the present study, an unexpected rapid changing pattern of RVA genotypes distribution in Brazilian population during three followed seasons is described. From January/2012 to December/2014, a total of 3441 fecal specimens were collected from collaborating centers across Southern, Southeastern and Midwest of Brazil. All specimens were screened for RVA using ELISA, and genotyped by RT-PCR. Differences in proportions were tested using Chi-Squares. A p-value of less than 0.05 was considered statistically significant. RVA was detected in 19.7% (677/3441). Among RVA positive cases (n=677), a total of 652 (96.3%) samples were successfully amplified by RT-PCR. G3P[8] remained prevalent in 2012 (37.6%, 69/185) and 2013 (40.1%, 74/186) (χ(2)=0.107, p=0.743), but declined markedly in 2014 (3.5%, 10/281) (χ(2)=71.770, p=0.000). G12P[8] was second highest strain in 2012 (22.7%, 42/185), decrease rapidly in 2013 (2.7%, 5/186) (χ(2)=26.224, p=0.000) and re-emerged as the predominant genotype in 2014 (86.6%, 243/281) (χ(2)=118.299, p=0.000). From July/2014, G12P[8] was the single genotype detected in all regions studied. The sudden emergence, spread and predominance of G12P[8] strain in Brazil, raised the hypothesis of a possible G12 outbreak being in progress. Nationally, the long term decline in gastroenteritis hospitalization observed in the country after RVA vaccine introduction was confirmed. Nevertheless, the sharp increase in diarrhea hospitalization prevalence from 2013 to 2014 observed in Southern and Southeastern regions is consistent with what appears to be an outbreak of G12P[8]. Continued surveillance is needed to verify the effectiveness of the RotarixTM vaccine in Brazil together with potential emergence of unusual genotypes.

  17. GENOTYPE BY ENVIRONMENT INTERACTIONS IN Pinus taeda L. IN SOUTH AND SOUTH-EAST BRAZIL

    Directory of Open Access Journals (Sweden)

    Estefano Paludzyszyn Filho

    2001-01-01

    Full Text Available Genotype x environment interactions of stem volume were investigated by assessing the variation in 46 open-pollinated loblolly pine (Pinus taeda L. families from first-generation cloned seed orchard in four genetic trials in the south and south-east Brazil. They were used to obtain least squares and restricted maximum likelihood (REML estimates of variance components. Familie-by- trial interaction effects were evaluated by adjusting the mixed univariate model that contained data of two and four places tested by the likelihood ratio test. Breeding values from local data (univarate procedure and predicted to the others sites (multivariate procedure were obtained from best linear unbiased prediction (BLUP. The adjusted and average (obtained from local and predicted to other sites breeding values were used to select parents and trees. The interaction effects and the adjusting of the mixed models were statistically significant, respectively, by F test and by likelihood ratio test. The loss of potential gain, sustained by not selecting the best families by site was 3.2%. For parents, the loss in mean productivity by indirect selection was respectively 2.3%. In the individual tree selection for seedling seed orchard, no loss of potential gain was observed when selection was carried by average genetic breeding values. For clonal seed orchard with the selection of ten more greater genetic breeding values trees, the interaction cause a inflation of 2% in the average productivity. In this case, the selection by average breeding values was the best procedure and may prove to be a useful tool, in selection stem volume, when genotype x environment interaction is significant.

  18. Human Papillomavirus Genotype Distribution in Invasive Cervical Cancer in Pakistan.

    Science.gov (United States)

    Loya, Asif; Serrano, Beatriz; Rasheed, Farah; Tous, Sara; Hassan, Mariam; Clavero, Omar; Raza, Muhammad; De Sanjosé, Silvia; Bosch, F Xavier; Alemany, Laia

    2016-01-01

    Few studies have assessed the burden of human papillomavirus (HPV) infection in Pakistan. We aim to provide specific information on HPV-type distribution in invasive cervical cancer (ICC) in the country. A total of 280 formalin-fixed paraffin-embedded tissue blocks were consecutively selected from Shaukat Khanum Memorial Cancer Hospital and Research Centre (Lahore, Pakistan). HPV-DNA was detected by SPF10 broad-spectrum PCR followed by DNA enzyme immunoassay and genotyping by LiPA25. HPV-DNA prevalence was 87.5% (95%CI: 83.0-91.1), with 96.1% of cases histologically classified as squamous cell carcinoma. Most of the HPV-DNA positive cases presented single infections (95.9%). HPV16 was the most common type followed by HPV18 and 45. Among HPV-DNA positive, a significantly higher contribution of HPV16/18 was detected in Pakistan (78.4%; 72.7-83.3), compared to Asia (71.6%; 69.9-73.4) and worldwide (70.8%; 69.9-71.8) and a lower contribution of HPVs31/33/45/52/58 (11.1%; 7.9-15.7 vs. 19.8%; 18.3-21.3 and 18.5%; 17.7-19.3). HPV18 or HPV45 positive ICC cases were significantly younger than cases infected by HPV16 (mean age: 43.3, 44.4, 50.5 years, respectively). A routine cervical cancer screening and HPV vaccination program does not yet exist in Pakistan; however, the country could benefit from national integrated efforts for cervical cancer prevention and control. Calculated estimations based on our results show that current HPV vaccine could potentially prevent new ICC cases. PMID:27483322

  19. Evidence for the co-circulation of dengue virus type 3 genotypes III and V in the Northern region of Brazil during the 2002-2004 epidemics

    Directory of Open Access Journals (Sweden)

    Meri Bordignon Nogueira

    2008-08-01

    Full Text Available The reintroduction of dengue virus type 3 (DENV-3 in Brazil in 2000 and its subsequent spread throughout the country was associated with genotype III viruses, the only DENV-3 genotype isolated in Brazil prior to 2002. We report here the co-circulation of two different DENV-3 genotypes in patients living in the Northern region of Brazil during the 2002-2004 epidemics. Complete genomic sequences of viral RNA were determined from these epidemics, and viruses belonging to genotypes V (Southeast Asia/South Pacific and III were identified. This recent co-circulation of different DENV-3 genotypes in South America may have implications for pathological and epidemiological dynamics.

  20. Group a rotavirus and norovirus genotypes circulating in the northeastern Brazil in the post-monovalent vaccination era.

    Science.gov (United States)

    Sá, Ana Caroline C; Gómez, Mariela M; Lima, Ila Fernanda N; Quetz, Josiane S; Havt, Alexandre; Oriá, Reinaldo B; Lima, Aldo A; Leite, José Paulo G

    2015-09-01

    Group A rotaviruses (RVA) and noroviruses (NoV) are the leading cause of acute gastroenteritis (AGE) worldwide. Childhood diarrhea deaths and hospital admissions have declined since the introduction of the monovalent (G1P[8]) vaccine (Rotarix(®) [RV1]) in the National Immunization Program in Brazil in 2006. This study aims to investigate the epidemiological profile of NoV and RVA infections from children with AGE in the Northeastern region of Brazil in the post vaccine season. Two-hundred fecal samples collected from children up to 10 years old in Fortaleza, Ceará between 2008-2009 were screened for the presence of RVA and NoV. Positive samples were genotyped and sequenced. The RVA screening revealed 12% prevalence and all RVA strains belonged to G2P[4] genotype. Phylogenetic analysis based on the 11 RVA genome segments sequenced from eight samples revealed a DS-1-like genotype constellation: I2-R2-C2-M2-A2-N2-T2-E2-H2. For NoV screening, the prevalence observed was 17% and the following genotypes were detected: GII.4 (59%), GII.12 (17%), GII.6 (9%), GII.3 (6%), and GII.? (9%). At least four different NoVs genotypes and two RVA G2P[4] variants were identified circulating in the Northeastern region of Brazil. RVA phylogenetic analysis suggests that the RVA G2P[4] strains might have originated from intragenogroup reassortment events. Whether the genetic modifications observed in these contemporary G2P[4] RVA strains may impact the long-term effectiveness of the current vaccination programs remains to be explored. These data reinforce the importance of surveillance for monitoring the emergence of new strains of RVA and NoV and their impact on cases of acute gastroenteritis.

  1. Hepatitis C virus genotypes distribution and transmission risk factors in Luxembourg from 1991 to 2006

    Institute of Scientific and Technical Information of China (English)

    2008-01-01

    AIM: To analyze the Hepatitis C virus (HCV) genotype distribution and transmission risk factors in a population of unselected patients in Luxembourg. METHODS: Epidemiological information (gender, age and transmission risks) were collected from 802patients newly diagnosed for hepatitis C and living in Luxembourg, among whom 228 patients referred from prison. Genotyping using 5'noncoding (5'NC) sequencing was performed. We compared categorical data using the Fisher's exact F-test and odds ratios (OR) were calculated for evaluating association of HCV genotype and risk factors. RESULTS: The sex ratio was predominantly male (2.2) and individuals aged less than 40 years represented 49.6% of the population. Genotype l was predominant (53.4%) followed by genotype 3 (33%). Among risk factors, intravenous drug usage (IVDU) was the most frequently reported (71.4%) followed by medical-related transmission (17.6%) including haernophilia, transfusion recipients and other nosocomial reasons. Genotype 3was significantly associated to IVDU (OR=4.84,P<0.0001= whereas genotype l was significantly associated with a medical procedure (OR = 2.42,P<0.001=.The HCV genotype distribution from inmate patients differed significantly from the rest of the population (Chi-square test with four degrees of freedom, P<0.0001= with a higher frequency of genotype 3 (46.5% vs 27.5%) and a lower frequency of genotype 1 and 4 (44.7% vs 56.8%and 5.3% vs 9.6%,respectively).IVDU was nearly exclusively reported as a risk factor in prison. CONCLUSION: We report the first description of the HCV genotype distribution in Luxembourg. The repartition is similar to other European countries, with one of the highest European prevalence rates of genotype 3 (33%). Since serology screening became available in 1991,IVDU remains the most common way of HCV transmission in Luxembourg.

  2. Distribution of HBV genotypes among HBV carriers in Benin:phylogenetic analysis and virological characteristics of HBV genotype E

    Institute of Scientific and Technical Information of China (English)

    Kei Fujiwara; Atsushi Ozasa; Yuko Sakamoto; Isao Arita; Ahmed El-Gohary; Agossou Benoit; Sophie I Ogoundele-Akplogan; Namiko Yoshihara; Ryuzo Ueda; Masashi Mizokami; Yasuhito Tanaka; Etsuro Orito; Tomoyoshi Ohno; Takanobu Kato; Kanji Sugihara; Izumi Hasegawa; Mayumi Sakurai; Kiyoaki Ito

    2005-01-01

    AIM: To determine the distribution of Hepatitis B virus (HBV) genotypes in Benin, and to clarify the virological characteristics of the dominant genotype.METHODS: Among 500 blood donors in Benin, 21 HBsAg-positive donors were enrolled in the study. HBV genotypes were determined by enzyme immunoassay and restriction fragment length polymorphism. Complete genome sequences were determined by PCR and direct sequencing.RESULTS: HBV genotype E (HBV/E) was detected in 20/21 (95.2%), and HBV/A in 1/21 (4.8%). From the age-specific prevalence of HBeAg to anti-HBe seroconversion (SC) in 19 HBV/E subjects, SC was estimated to occur frequently in late teens in HBV/E.The comparison of four complete HBV/E genomes from HBeAg-positive subjects in this study and five HBV/E sequences recruited from the database revealed that HBV/E was distributed throughout West Africa with very low genetic divers ity (nucleotide homology 96.7-99.2%).Based on the sequences in the basic core promoter (BCP)to precore region of the nine HBV/E isolates compared to those of the other genotypes, a nucleotide substitution in the BCP, G1757A, was observed in HBV/E.CONCLUSION: HBV/E is predominant in the Republic of Benin, and SC is estimated to occur in late teens in HBV/E. The specific nucleotide substitution G1757A in BCP, which might influence the virological characteristics,is observed in HBV/E.

  3. Distribution and heterogeneity of hepatitis C genotypes in hepatitis patients in Cameroon.

    Science.gov (United States)

    Pasquier, Christophe; Njouom, Richard; Ayouba, Ahidjo; Dubois, Martine; Sartre, Michèle Tagni; Vessière, Aurelia; Timba, Isabelle; Thonnon, Jocelyn; Izopet, Jacques; Nerrienet, Eric

    2005-11-01

    Hepatitis C virus infects humans world-wide. The virus genome varies greatly and it has several genotypes. HCV infection is highly prevalent in Central Africa and Cameroon. Initial studies on the genetic variability of HCV showed infection with HCV genotypes 1, 2, and 4. We have now sequenced the NS5b and E2 regions of 156 HCV isolates collected from patients presenting for diagnosis in Yaounde and used the data to describe the distribution of HCV genotypes and subtypes in patients with hepatitis in Cameroon. Genotype 1 was more frequent than Genotypes 4 and 2. Genotypes 1 and 4 were highly heterogeneous, containing many subtypes described previously (1b, 1c, 1e, 1h, 1l, 4f, 4t, 4p, 4k) and unsubtyped groups. There was a systematic phylogenetic concordance between NS5b and E2 sequence clustering. The Genotype 2 sequences did not vary. Neither subject age nor gender influenced HCV distribution. HCV Genotypes 1 and 4 are very heterogeneous in Cameroon, perhaps due to ancient infections. The homogeneity of HCV Genotype 2 indicates its more recent introduction from western Africa.

  4. Distribution and heterogeneity of hepatitis C genotypes in hepatitis patients in Cameroon.

    Science.gov (United States)

    Pasquier, Christophe; Njouom, Richard; Ayouba, Ahidjo; Dubois, Martine; Sartre, Michèle Tagni; Vessière, Aurelia; Timba, Isabelle; Thonnon, Jocelyn; Izopet, Jacques; Nerrienet, Eric

    2005-11-01

    Hepatitis C virus infects humans world-wide. The virus genome varies greatly and it has several genotypes. HCV infection is highly prevalent in Central Africa and Cameroon. Initial studies on the genetic variability of HCV showed infection with HCV genotypes 1, 2, and 4. We have now sequenced the NS5b and E2 regions of 156 HCV isolates collected from patients presenting for diagnosis in Yaounde and used the data to describe the distribution of HCV genotypes and subtypes in patients with hepatitis in Cameroon. Genotype 1 was more frequent than Genotypes 4 and 2. Genotypes 1 and 4 were highly heterogeneous, containing many subtypes described previously (1b, 1c, 1e, 1h, 1l, 4f, 4t, 4p, 4k) and unsubtyped groups. There was a systematic phylogenetic concordance between NS5b and E2 sequence clustering. The Genotype 2 sequences did not vary. Neither subject age nor gender influenced HCV distribution. HCV Genotypes 1 and 4 are very heterogeneous in Cameroon, perhaps due to ancient infections. The homogeneity of HCV Genotype 2 indicates its more recent introduction from western Africa. PMID:16173014

  5. Distribution of Candida albicans genotypes among family members

    Science.gov (United States)

    Mehta, S. K.; Stevens, D. A.; Mishra, S. K.; Feroze, F.; Pierson, D. L.

    1999-01-01

    Thirty-three families (71 subjects) were screened for the presence of Candida albicans in mouthwash or stool specimens; 12 families (28 subjects) were culture-positive for this yeast. An enrichment procedure provided a twofold increase in the recovery of C. albicans from mouthwash specimens. Nine of the twelve culture-positive families had two positive members each, two families had three positive members each, and one family had four positive members. Genetic profiles were obtained by three methods: pulsed-field gel electrophoresis; restriction endonuclease analysis, and random amplification of polymorphic DNA analysis. DNA fingerprinting of C. albicans isolated from one body site three consecutive times revealed that each of the 12 families carried a distinct genotype. No two families shared the same strain, and two or more members of a family commonly shared the same strain. Intrafamily genotypic identity (i.e., each member within the family harbored the same strain) was demonstrated in six families. Genotypes of isolates from husband and wife differed from one another in five families. All three methods were satisfactory in determining genotypes; however, we concluded that restriction endonuclease analysis provided adequate resolving power.

  6. Genotypic diversity, pathogenic potential and the resistance profile of Salmonella Typhimurium strains isolated from humans and food from 1983 to 2013 in Brazil.

    Science.gov (United States)

    Almeida, Fernanda; Medeiros, Marta Inês Cazentini; Rodrigues, Dália dos Prazeres; Falcão, Juliana Pfrimer

    2015-11-01

    Salmonella enterica subsp. enterica serovar Typhimurium is one of the leading serovars that causes salmonellosis worldwide. However, few studies have molecularly characterized S. Typhimurium strains in Brazil. In this study, we genotyped 92 S. Typhimurium strains isolated from humans (43) and food (49) between 1983 and 2013 in Brazil using PFGE, multiple-locus variable number of tandem repeats analysis (MLVA) and enterobacterial repetitive intergenic consensus PCR (ERIC-PCR). Moreover, we assessed the frequency of 12 virulence markers by PCR and the resistance profile against 12 antimicrobials. More than 85.8% of the strains studied carried 11 of the virulence markers or more. Thirty-three strains (25%) were multidrug resistant (MDR). The 92 S. Typhimurium studied were grouped by PFGE as PFGE-A, PFGE-B1 and PFGE-B2; by MLVA as MLVA-A, MLVA-B1 and MLVA-B2; and, finally, by ERIC-PCR as ERIC-A and ERIC-B. The strains isolated from humans before the mid-1990s were allocated to all clusters. The strains isolated from humans after the mid-1990s were distributed in the PFGE-B1, MLVA-B1, MLVA-B2 and ERIC-A clusters. The strains isolated from food were distributed in all clusters, except in PFGE-B2. All typing results suggested that the S. Typhimurium strains of human clinical origin isolated before the mid-1990s were genetically more diverse, which might indicate the selection of a more adapted S. Typhimurium subtype after Salmonella Enteritidis became the most prevalent serovar in Brazil. Regarding strains isolated from food, the results suggest the current circulation of more than one subtype. Furthermore, the high frequency of virulence genes and the presence of MDR strains reinforces their potential hazard for humans and the risk of their presence in foods in Brazil.

  7. HCV genotype distribution and possible transmission risks in Lahore, Pakistan

    Institute of Scientific and Technical Information of China (English)

    Waqar; Ahmad; Bushra; Ijaz; Fouzia; Tahir; Javed; Shah; Jahan; Imran; Shahid; Fawad; Mumtaz; Khan; Sajida; Hassan

    2010-01-01

    AIM: To investigate the prevalence of hepatitis C virus (HCV) genotypes and their association with possible transmission routes in the general population of Lahore, as the data exclusively related to this city is limited. METHODS: Complete data regarding patient's history, possible route of infection and biochemical tests was collected from the public hospital for 1364 patients. SPSS version 16 windows software was used for data analysis by univariate and multivariate techniques. RESULTS: Age range ≤ 40 yea...

  8. The Evolving Genotypic Profile of HIV-1 Mutations Related to Antiretroviral Treatment in the North Region of Brazil.

    Science.gov (United States)

    Lopes, Carmen Andréa F; Soares, Marcelo A; Falci, Diego R; Sprinz, Eduardo

    2015-01-01

    HIV related mutations can be associated with decreased susceptibility to antiretrovirals and treatment failures. There is scarce information about HIV mutations in persons failing HIV treatment in North of Brazil. Our aim was to evaluate evolution of HIV subtypes and mutations patterns related to antiretroviral therapy in this region. We investigated HIV resistance profile in adults failing antiretroviral regimen in Northern Brazil from January, 2004, through December, 2013. Genotype data was evaluated through Stanford University algorithm. There were 377 genotypes from different individuals to evaluate. Resistance mutations were similar to worldwide reports and related to antiretroviral exposure. Most prevalent mutations in the reverse transcriptase gene were M184V (80.1%) and K130N (40.6%). Thymidine associated mutations were more frequent in multiexperienced patients. Most common protease mutations were M46I, V82A, I54V, L90M, I84V, M46L, and L76V. Subtype B was the most prevalent (90.7%). There were differences between subtypes B and non-B mutations. We documented for the first time subtypes and patterns of HIV associated mutations in Northern Brazil. A1 subtype was identified for the first time in this area. Depending on drug regimen and how experienced the patient is, an empirical switch of a failing antiretroviral treatment could be a reasonable option.

  9. Analysis of Mycobacterium tuberculosis Genotypic Lineage Distribution in Chile and Neighboring Countries.

    Science.gov (United States)

    Lagos, Jaime; Couvin, David; Arata, Loredana; Tognarelli, Javier; Aguayo, Carolina; Leiva, Tamara; Arias, Fabiola; Hormazabal, Juan Carlos; Rastogi, Nalin; Fernández, Jorge

    2016-01-01

    Tuberculosis (TB), caused by the pathogen Mycobacterium tuberculosis (MTB), remains a disease of high importance to global public health. Studies into the population structure of MTB have become vital to monitoring possible outbreaks and also to develop strategies regarding disease control. Although Chile has a low incidence of MTB, the current rates of migration have the potential to change this scenario. We collected and analyzed a total of 458 M. tuberculosis isolates (1 isolate per patient) originating from all 15 regions of Chile. The isolates were genotyped using the spoligotyping method and the data obtained were analyzed and compared with the SITVIT2 database. A total of 169 different patterns were identified, of which, 119 patterns (408 strains) corresponded to Spoligotype International Types (SITs) and 50 patterns corresponded to orphan strains. The most abundantly represented SITs/lineages were: SIT53/T1 (11.57%), SIT33/LAM3 (9.6%), SIT42/LAM9 (9.39%), SIT50/H3 (5.9%), SIT37/T3 (5%); analysis of the spoligotyping minimum spanning tree as well as spoligoforest were suggestive of a recent expansion of SIT42, SIT50 and SIT37; all of which potentially evolved from SIT53. The most abundantly represented lineages were LAM (40.6%), T (34.1%) and Haarlem (13.5%). LAM was more prevalent in the Santiago (43.6%) and Concepción (44.1%) isolates, rather than the Iquique (29.4%) strains. The proportion of X lineage was appreciably higher in Iquique and Concepción (11.7% in both) as compared to Santiago (1.6%). Global analysis of MTB lineage distribution in Chile versus neighboring countries showed that evolutionary recent lineages (LAM, T and Haarlem) accounted together for 88.2% of isolates in Chile, a pattern which mirrored MTB lineage distribution in neighboring countries (n = 7378 isolates recorded in SITVIT2 database for Peru, Brazil, Paraguay, and Argentina; and published studies), highlighting epidemiological advantage of Euro-American lineages in this region

  10. Analysis of Mycobacterium tuberculosis Genotypic Lineage Distribution in Chile and Neighboring Countries

    Science.gov (United States)

    Lagos, Jaime; Couvin, David; Arata, Loredana; Tognarelli, Javier; Aguayo, Carolina; Leiva, Tamara; Arias, Fabiola; Hormazabal, Juan Carlos; Rastogi, Nalin; Fernández, Jorge

    2016-01-01

    Tuberculosis (TB), caused by the pathogen Mycobacterium tuberculosis (MTB), remains a disease of high importance to global public health. Studies into the population structure of MTB have become vital to monitoring possible outbreaks and also to develop strategies regarding disease control. Although Chile has a low incidence of MTB, the current rates of migration have the potential to change this scenario. We collected and analyzed a total of 458 M. tuberculosis isolates (1 isolate per patient) originating from all 15 regions of Chile. The isolates were genotyped using the spoligotyping method and the data obtained were analyzed and compared with the SITVIT2 database. A total of 169 different patterns were identified, of which, 119 patterns (408 strains) corresponded to Spoligotype International Types (SITs) and 50 patterns corresponded to orphan strains. The most abundantly represented SITs/lineages were: SIT53/T1 (11.57%), SIT33/LAM3 (9.6%), SIT42/LAM9 (9.39%), SIT50/H3 (5.9%), SIT37/T3 (5%); analysis of the spoligotyping minimum spanning tree as well as spoligoforest were suggestive of a recent expansion of SIT42, SIT50 and SIT37; all of which potentially evolved from SIT53. The most abundantly represented lineages were LAM (40.6%), T (34.1%) and Haarlem (13.5%). LAM was more prevalent in the Santiago (43.6%) and Concepción (44.1%) isolates, rather than the Iquique (29.4%) strains. The proportion of X lineage was appreciably higher in Iquique and Concepción (11.7% in both) as compared to Santiago (1.6%). Global analysis of MTB lineage distribution in Chile versus neighboring countries showed that evolutionary recent lineages (LAM, T and Haarlem) accounted together for 88.2% of isolates in Chile, a pattern which mirrored MTB lineage distribution in neighboring countries (n = 7378 isolates recorded in SITVIT2 database for Peru, Brazil, Paraguay, and Argentina; and published studies), highlighting epidemiological advantage of Euro-American lineages in this region

  11. Distribution of Hepatitis C virus genotypes in city of Mashhad, North-east of Iran

    Directory of Open Access Journals (Sweden)

    M Rastin

    2014-01-01

    Full Text Available Purpose: Six major hepatitis C virus genotypes have been characterised, which vary in their geographical distribution. The prevalence of hepatitis C virus (HCV in an area is not constant, and depends on the changes in route of infection, which may change over time. In this study, the distribution of HCV genotypes in Mashhad, the capital of Razavi Khorasan province in north-east of Iran was investigated. Mashhad is a holy city of Shiate Moslems, which attracts more than 20 million tourists and pilgrims every year. Materials and Methods: Two hundred and seventy-eight HCV infected subjects (227 males and 51 females were included in this study. HCV genotypes were analysed by type specific reverse transcriptase polymerase chain reaction (RT-PCR. Results: Genotype 3a was detected in 49.6%, 1a in 36.3%, 1b in 12.6% and 2a in 0.4%. Two HCV genotypes were detected in 1.1% cases; 1a +3a in 1%, 3a + 1b in 0.4%. Genotypes 2b and 3b were not detected in any samples. Conclusion: We demonstrated that despite the previous reports on the frequency of HCV genotypes in Iran, 3a is the predominant genotype in Mashhad.

  12. Prevalence and genotype distribution of rotaviruses in children with gastroenteritis in Rize province

    Directory of Open Access Journals (Sweden)

    Selim Dereci

    2015-07-01

    Full Text Available Determination of the distribution of rotavirus genotypes is essential for understanding the epidemiology of this virus responsible for nearly half a million of deaths in patients with gastroenteritis worldwide. In the present study, we aimed to genotype the rotavirus strains isolated from diarrheal stool samples in children under 5 years old. A total of 1297 fecal samples were collected, and rotavirus antigen was detected in 73 of these samples. Antigen-positive samples were transferred to the Public Health Agency of Turkey, Molecular Microbiology Research Laboratory, and were tested for determination of genotypes G and P using semi-nested multiplex polymerase chain reaction method performed with consensus- and genotype-specific primers. Twelve specimens were found to be negative for rotavirus in genotyping method. All the positive-strains were in G1-4, G8-9, P(4, P(8, and P(9 genotypes. The most frequent GP genotype combinations were found to be G9P(8 in 21 strains (34.4%, G2P(4 in 14 strains (23.0%, and G1P(8 in 12 strains (19.7%. We found 10 distinct genotypes amongst a total of 61 strains. Among the strains isolated and genotyped in our study, 90.2% (55/61 and 67.2% (41/61 have already been included in the two existing commercial vaccines. In conclusion, these findings implicate the necessity of development of region-specific vaccines after evaluation of the local genotype distribution. Further studies on the large number of rotavirus strains would contribute to this process. 

  13. Prevalence and genotyping of hepatitis C virus in blood donors in the state of Pará, Northern Brazil

    Directory of Open Access Journals (Sweden)

    Aldemir B Oliveira-Filho

    2010-02-01

    Full Text Available Given the scarcity of epidemiological information on hepatitis C virus (HCV infection in Northern Brazil, we determined the prevalence and genotypic frequency in blood donors in the state of Pará (PA. Blood samples from all of the blood donors at the Fundação HEMOPA (blood bank of PA from 2004-2006 were screened for the presence of antibodies to anti-HCV and samples seroreactive to anti-HCV were further tested for HCV RNA using real-time PCR. In total, 116 HCV-RNA samples were genotyped, based on maximum likelihood phylogenetic analyses, using BioEdit, Modelgenerator, PHYML and FigTree software. The population consisted of 242,726 volunteers who donated blood from 2004-2006; the most common subgroup was males between the ages of 18-29 years old (37.30%. Within the whole group, 1,112 blood donors (0.46% had indeterminate or positive serology; among these, 28.78% were males whose ages ranged from 18-29 years. A diagnosis of chronic HCV infection was confirmed for 304 donors (60.20% males; 66.45% were 30-49 years old, resulting in a prevalence of HCV RNA in 0.13% of the samples (304 of 242,726. HCV genotyping revealed a high frequency of genotype 1 (108/116 followed by genotype 3 (8/116. This study found HCV infection to be relatively infrequent in PA; genotype 1 was most commonly isolated. This information can help guide prevention and control policies aimed at efficient diagnosis and control measures.

  14. The Distribution of Hepatitis C Virus Genotypes in Patients with Chronic Hepatitis C Infection

    Directory of Open Access Journals (Sweden)

    Sevin Kırdar

    2015-12-01

    Full Text Available Objective: Hepatitis C virus (HCV infection represents a major public health problem worldwide. HCV can cause chronic hepatitis infection which may ultimately result in cirrhosis and hepatocellular carcinoma. Seven major genotypes and more than 100 subtypes of HCV are shown by sequence analysis. Genotype 1 is associated with more severity of liver disease than genotypes 2 and 3 and sustained response totreatment is known to be less. In this study, we aimed to determine the HCV genotype distribution in chronic hepatitis C patients. Materials and Methods: A total of 50 patients with chronic HCV infection who attended the Microbiology Laboratory at Adnan Menderes University Hospital between August 2007 and December 2010 found to be positive for anti-HCV and HCV-RNA were included in the study. Anti-HCV testing was performed using microparticle Enzyme-Linked immunosorbent assay test kit (Murex Anti-HCV version 4, UK with autoanalyser (Grifols Triturus, Spain. The quantification of serum HCV-RNA was carried out by a realtime polymerase chain reaction method with two different systems (Cobas TaqMan HCV, Roche Diagnostics, Germany and RotorGene 6000,Corbett Research, USA. HCV genotype analysis was performed by using a kit (HCV-TS; AB Analitica, Italy based on the reverse hybridization of 5’-untranslated region and amplified products with genotype-specific probes. Results: The mean age of the 50 chronic hepatitis C patients [27 (54% female, and 23 (46% male] was 57.1±14.3 years. Genotype 1b was found in 36 (72% subjects, genotype 1a in nine (18%, genotype 2b in one (2%, genotype 3 in one (2%, and genotype 1a/1b was found in three (6% patients. No statistically significant difference was detected in HCV-RNA quantities and anti-HCV index between HCV genotypes (p>0.05. Conclusion: Compatible with the previous data obtained in Turkey, genotype 1b was found to be the most common HCV genotype in patients with chronic hepatitis C followed in our hospital.

  15. Genotypic distribution of hepatitis C virus in Thailand and Southeast Asia.

    Science.gov (United States)

    Wasitthankasem, Rujipat; Vongpunsawad, Sompong; Siripon, Nipaporn; Suya, Chutima; Chulothok, Phrutsada; Chaiear, Kasemporn; Rujirojindakul, Pairaya; Kanjana, Sawan; Theamboonlers, Apiradee; Tangkijvanich, Pisit; Poovorawan, Yong

    2015-01-01

    The majority of hepatitis C virus (HCV) infection results in chronic infection, which can lead to liver cirrhosis and hepatocellular carcinoma. Global burden of hepatitis C virus (HCV) is estimated at 150 million individuals, or 3% of the world's population. The distribution of the seven major genotypes of HCV varies with geographical regions. Since Asia has a high incidence of HCV, we assessed the distribution of HCV genotypes in Thailand and Southeast Asia. From 588 HCV-positive samples obtained throughout Thailand, we characterized the HCV 5' untranslated region, Core, and NS5B regions by nested PCR. Nucleotide sequences obtained from both the Core and NS5B of these isolates were subjected to phylogenetic analysis, and genotypes were assigned using published reference genotypes. Results were compared to the epidemiological data of HCV genotypes identified within Southeast Asian. Among the HCV subtypes characterized in the Thai samples, subtype 3a was the most predominant (36.4%), followed by 1a (19.9%), 1b (12.6%), 3b (9.7%) and 2a (0.5%). While genotype 1 was prevalent throughout Thailand (27-36%), genotype 3 was more common in the south. Genotype 6 (20.9%) constituted subtype 6f (7.8%), 6n (7.7%), 6i (3.4%), 6j and 6m (0.7% each), 6c (0.3%), 6v and 6xa (0.2% each) and its prevalence was significantly lower in southern Thailand compared to the north and northeast (p = 0.027 and p = 0.030, respectively). Within Southeast Asia, high prevalence of genotype 6 occurred in northern countries such as Myanmar, Laos, and Vietnam, while genotype 3 was prevalent in Thailand and Malaysia. Island nations of Singapore, Indonesia and Philippines demonstrated prevalence of genotype 1. This study further provides regional HCV genotype information that may be useful in fostering sound public health policy and tracking future patterns of HCV spread. PMID:25962112

  16. Genotypic distribution of hepatitis C virus in Thailand and Southeast Asia.

    Directory of Open Access Journals (Sweden)

    Rujipat Wasitthankasem

    Full Text Available The majority of hepatitis C virus (HCV infection results in chronic infection, which can lead to liver cirrhosis and hepatocellular carcinoma. Global burden of hepatitis C virus (HCV is estimated at 150 million individuals, or 3% of the world's population. The distribution of the seven major genotypes of HCV varies with geographical regions. Since Asia has a high incidence of HCV, we assessed the distribution of HCV genotypes in Thailand and Southeast Asia. From 588 HCV-positive samples obtained throughout Thailand, we characterized the HCV 5' untranslated region, Core, and NS5B regions by nested PCR. Nucleotide sequences obtained from both the Core and NS5B of these isolates were subjected to phylogenetic analysis, and genotypes were assigned using published reference genotypes. Results were compared to the epidemiological data of HCV genotypes identified within Southeast Asian. Among the HCV subtypes characterized in the Thai samples, subtype 3a was the most predominant (36.4%, followed by 1a (19.9%, 1b (12.6%, 3b (9.7% and 2a (0.5%. While genotype 1 was prevalent throughout Thailand (27-36%, genotype 3 was more common in the south. Genotype 6 (20.9% constituted subtype 6f (7.8%, 6n (7.7%, 6i (3.4%, 6j and 6m (0.7% each, 6c (0.3%, 6v and 6xa (0.2% each and its prevalence was significantly lower in southern Thailand compared to the north and northeast (p = 0.027 and p = 0.030, respectively. Within Southeast Asia, high prevalence of genotype 6 occurred in northern countries such as Myanmar, Laos, and Vietnam, while genotype 3 was prevalent in Thailand and Malaysia. Island nations of Singapore, Indonesia and Philippines demonstrated prevalence of genotype 1. This study further provides regional HCV genotype information that may be useful in fostering sound public health policy and tracking future patterns of HCV spread.

  17. Genotyping of human parvovirus B19 in clinical samples from Brazil and Paraguay using heteroduplex mobility assay, single-stranded conformation polymorphism and nucleotide sequencing

    Directory of Open Access Journals (Sweden)

    Marcos César Lima de Mendonça

    2011-06-01

    Full Text Available Heteroduplex mobility assay, single-stranded conformation polymorphism and nucleotide sequencing were utilised to genotype human parvovirus B19 samples from Brazil and Paraguay. Ninety-seven serum samples were collected from individuals presenting with abortion or erythema infectiosum, arthropathies, severe anaemia and transient aplastic crisis; two additional skin samples were collected by biopsy. After the procedure, all clinical samples were classified as genotype 1.

  18. Genotyping of human parvovirus B19 in clinical samples from Brazil and Paraguay using heteroduplex mobility assay, single-stranded conformation polymorphism and nucleotide sequencing.

    Science.gov (United States)

    Mendonça, Marcos César Lima de; Ferreira, Ana Maria de Amorim; Santos, Marta Gonçalves Matos dos; Oviedo, Elva Cristina; Bello, Maria Sônia Dal; Siqueira, Marilda Mendonça; Maceira, Juan Manuel Piñeiro; von Hubinger, Maria Genoveva; Couceiro, José Nelson dos Santos Silva

    2011-06-01

    Heteroduplex mobility assay, single-stranded conformation polymorphism and nucleotide sequencing were utilised to genotype human parvovirus B19 samples from Brazil and Paraguay. Ninety-seven serum samples were collected from individuals presenting with abortion or erythema infectiosum, arthropathies, severe anaemia and transient aplastic crisis; two additional skin samples were collected by biopsy. After the procedure, all clinical samples were classified as genotype 1.

  19. THE DOHA DEVELOPMENT AGENDA AND BRAZIL: A CLOSER LOOK INTO THE DISTRIBUTIONAL IMPACTS INSIDE AGRICULTURE

    OpenAIRE

    Ferreira Filho, Joaquim Bento de Souza; Horridge, Mark

    2006-01-01

    In this paper we extend previous results about how trade integration can affect poverty and income distribution in Brazil. To assess the impacts of a Doha Development Agenda (DDA) scenario on poverty and income distribution in Brazil, a 2 computable general equilibrium model (CGE) of Brazil was used, linked to a microsimulation (MS) model. This method was proposed by Ferreira Filho and Horridge, and guarantees consistency between both models. The model comprises 112,055 Brazilian households a...

  20. Phenotypic and genotypic characterization of two Toxoplasma gondii isolates in free-range chickens from Uberlândia, Brazil.

    Science.gov (United States)

    Lopes, C S; Franco, P S; Silva, N M; Silva, D A O; Ferro, E A V; Pena, H F J; Soares, R M; Gennari, S M; Mineo, J R

    2016-07-01

    The aim of this study was to determine the seroprevalence of Toxoplasma gondii infection in free-range chickens from Uberlândia, Minas Gerais state, Brazil, and characterize the genotypic and phenotypic features of two isolates of this parasite, considering the importance of these hosts in the epidemiology of toxoplasmosis. Serum samples from 108 free-range chickens were obtained from ten different districts, and submitted to the modified agglutination test (MAT) for the presence of anti-T. gondii antibodies, and brain and heart tissue samples from infected chickens were processed for mouse bioassay. An overall seroprevalence of 71·3% was found and antibody titres ranged from 16 to 4096. After confirmation of seropositivity by mouse bioassay, the determination of the T. gondii genotypes of two isolates was performed by PCR-RFLP, using primers for the following markers: SAG1, SAG2, SAG3, BTUB, GRA6, c22-8, c29-2, L358, PK1, new SAG2, Apico and CS3. These T. gondii isolates, designated TgChBrUD1and TgChBrUD2, were obtained from heart samples of free-range chickens. The TgChBrUD1 isolate belonged to ToxoDB PCR-RFLP genotype 11 and the TgChBrUD2 isolate belonged to ToxoDB PCR-RFLP genotype 6. Both isolates demonstrated high virulence in a rodent model, with the TgChBrUD1 isolate able to induce brain cysts, in accord with its pattern of multiplication rates in human fibroblast culture. Taken together, these results reveal high prevalence of T. gondii infection in free-range chickens throughout Uberlândia, indicating an important degree of oocyst environmental contamination and the existence of considerable risk for T. gondii transmission to humans by consumption of free-range chicken as a food source.

  1. Prevalence and genotype distribution of Pneumocystis jirovecii in Cuban infants and toddlers with whooping cough.

    Science.gov (United States)

    Monroy-Vaca, Ernesto X; de Armas, Yaxsier; Illnait-Zaragozí, María T; Toraño, Gilda; Diaz, Raúl; Vega, Dania; Alvarez-Lam, Ileana; Calderón, Enrique J; Stensvold, Christen R

    2014-01-01

    This study describes the prevalence and genotype distribution of Pneumocystis jirovecii obtained from nasopharyngeal (NP) swabs from immunocompetent Cuban infants and toddlers with whooping cough (WC). A total of 163 NP swabs from 163 young Cuban children with WC who were admitted to the respiratory care units at two pediatric centers were studied. The prevalence of the organism was determined by a quantitative PCR (qPCR) assay targeting the P. jirovecii mitochondrial large subunit (mtLSU) rRNA gene. Genotypes were identified by direct sequencing of mtLSU ribosomal DNA (rDNA) and restriction fragment length polymorphism (RFLP) analysis of the dihydropteroate synthase (DHPS) gene amplicons. qPCR detected P. jirovecii DNA in 48/163 (29.4%) samples. mtLSU rDNA sequence analysis revealed the presence of three different genotypes in the population. Genotype 2 was most common (48%), followed in prevalence by genotypes 1 (23%) and 3 (19%); mixed-genotype infections were seen in 10% of the cases. RFLP analysis of DHPS PCR products revealed four genotypes, 18% of which were associated with resistance to sulfa drugs. Only contact with coughers (prevalence ratio [PR], 3.51 [95% confidence interval {CI}, 1.79 to 6.87]; P = 0.000) and exposure to tobacco smoke (PR, 1.82 [95% CI, 1.14 to 2.92]; P = 0.009) were statistically associated with being colonized by P. jirovecii. The prevalence of P. jirovecii in infants and toddlers with WC and the genotyping results provide evidence that this population represents a potential reservoir and transmission source of P. jirovecii.

  2. Temporal distribution of tuberculosis in the State of Amazonas, Brazil

    Directory of Open Access Journals (Sweden)

    Marlucia da Silva Garrido

    2015-06-01

    Full Text Available Tuberculosis (TB is one of the infectious diseases that contributes most to the morbidity and mortality of millions of people worldwide. Brazil is one of 22 countries that accounts for 80% of the tuberculosis global burden. The highest incidence rates in Brazil occur in the States of Amazonas and Rio de Janeiro. The aim of this study was to describe the temporal distribution of TB in the State of Amazonas. Between 2001 and 2011, 28,198 cases of tuberculosis were reported in Amazonas, distributed among 62 municipalities, with the capital Manaus reporting the highest (68.7% concentration of cases. Tuberculosis was more prevalent among males (59.3% aged 15 to 34 years old (45.5%, whose race/color was predominantly pardo (64.7% and who had pulmonary TB (84.3%. During this period, 81 cases of multidrug-resistant TB were registered, of which the highest concentration was reported from 2008 onward (p = 0.002. The municipalities with the largest numbers of indigenous individuals affected were São Gabriel da Cachoeira (93%, Itamarati (78.1%, and Santa Isabel do Rio Negro (70.1%. The future outlook for this region includes strengthening the TB control at the primary care level, by expanding diagnostic capabilities, access to treatment, research projects developed in collaboration with the Dr. Heitor Vieira Dourado Tropical Medicine Foundation .;Fundação de Medicina Tropical Dr. Heitor Vieira Dourado (FMT-HVD.; and financing institutions, such as the project for the expansion of the Clinical Research Center and the creation of a hospital ward for individuals with transmissible respiratory diseases, including TB.

  3. Genotyping of Leptospira directly in urine samples of cattle demonstrates a diversity of species and strains in Brazil.

    Science.gov (United States)

    Hamond, C; Pestana, C P; Medeiros, M A; Lilenbaum, W

    2016-01-01

    The aim of this study was to identify Leptospira in urine samples of cattle by direct sequencing of the secY gene. The validity of this approach was assessed using ten Leptospira strains obtained from cattle in Brazil and 77 DNA samples previously extracted from cattle urine, that were positive by PCR for the genus-specific lipL32 gene of Leptospira. Direct sequencing identified 24 (31·1%) interpretable secY sequences and these were identical to those obtained from direct DNA sequencing of the urine samples from which they were recovered. Phylogenetic analyses identified four species: L. interrogans, L. borgpetersenii, L. noguchii, and L. santarosai with the most prevalent genotypes being associated with L. borgpetersenii. While direct sequencing cannot, as yet, replace culturing of leptospires, it is a valid additional tool for epidemiological studies. An unexpected finding from this study was the genetic diversity of Leptospira infecting Brazilian cattle.

  4. Genotyping of Mycobacterium leprae present on Ziehl-Neelsen-stained microscopic slides and in skin biopsy samples from leprosy patients in different geographic regions of Brazil

    Directory of Open Access Journals (Sweden)

    Amanda Nogueira Brum Fontes

    2012-12-01

    Full Text Available We analysed 16 variable number tandem repeats (VNTR and three single-nucleotide polymorphisms (SNP in Mycobacterium leprae present on 115 Ziehl-Neelsen (Z-N-stained slides and in 51 skin biopsy samples derived from leprosy patients from Ceará (n = 23, Pernambuco (n = 41, Rio de Janeiro (n = 22 and Rondônia (RO (n = 78. All skin biopsies yielded SNP-based genotypes, while 48 of the samples (94.1% yielded complete VNTR genotypes. We evaluated two procedures for extracting M. leprae DNA from Z-N-stained slides: the first including Chelex and the other combining proteinase and sodium dodecyl sulfate. Of the 76 samples processed using the first procedure, 30.2% were positive for 16 or 15 VNTRs, whereas of the 39 samples processed using the second procedure, 28.2% yielded genotypes defined by at least 10 VNTRs. Combined VNTR and SNP analysis revealed large variability in genotypes, but a high prevalence of SNP genotype 4 in the Northeast Region of Brazil. Our observation of two samples from RO with an identical genotype and seven groups with similar genotypes, including four derived from residents of the same state or region, suggest a tendency to form groups according to the origin of the isolates. This study demonstrates the existence of geographically related M. leprae genotypes and that Z-N-stained slides are an alternative source for M. leprae genotyping.

  5. Tsallis statistics in the income distribution of Brazil

    Science.gov (United States)

    Soares, Abner D.; Moura, Newton J., Jr.; Ribeiro, Marcelo B.

    2016-07-01

    This paper discusses the empirical evidence of Tsallis statistical functions in the personal income distribution of Brazil. Yearly samples from 1978 to 2014 were linearized by the q-logarithm and straight lines were fitted to the entire range of the income data in all samples, producing a two-parameters-only single function representation of the whole distribution in every year. The results showed that the time evolution of the parameters is periodic and plotting one in terms of the other reveals a cycle mostly clockwise. It was also found that the empirical data oscillate periodically around the fitted straight lines with the amplitude growing as the income values increase. Since the entire income data range can be fitted by a single function, this raises questions on previous results claiming that the income distribution is constituted by a well defined two-classes-base income structure, since such a division in two very distinct income classes might not be an intrinsic property of societies, but a consequence of an a priori fitting-choice procedure that may leave aside possibly important income dynamics at the intermediate levels.

  6. ISOLATION AND GENOTYPING OF Toxoplasma gondii IN SERONEGATIVE URBAN RATS AND PRESENCE OF ANTIBODIES IN COMMUNICATING DOGS IN BRAZIL.

    Science.gov (United States)

    Ruffolo, Bruno Bergamo; Toledo, Roberta dos Santos; Martins, Felippe Danyel Cardoso; Bugni, Felipe Monteiro; Costa, Letícia da; Marana, Elizabete Regina Marangoni; Navarro, Italmar Teodorico; Garcia, João Luis; Su, Chunlei; Freire, Roberta Lemos

    2016-01-01

    The role of rodents in the epidemiology of toxoplasmosis was investigated in Londrina, Paraná State, Brazil. One hundred and eighty-one Rattus rattus and one Mus musculus were caught in 37 places. Blood and tissues were collected and submitted to the indirect fluorescence antibody test (IFAT) and the bioassay. Serum samples from 61 contacting dogs were also collected. Sixteen rats (8.8%) were positive for Toxoplasma gondii, but just two of them were positive by serology and bioassay test. Antibodies were found in nine (4.9%) rats. Tissues of nine rats bioassayed were positive and four isolates were obtained. Restriction fragment length polymorphism (RFLP) analysis was performed using 12 markers (SAG1, SAG2, SAG2-alt, C22-8, C29-2, L358, PK1, BTUB, GRA6, SAG3, Apico, CS3). Genotyping revealed that the four strains isolated from this study have been isolated before in cats and chickens from Brazil. None of the isolates was identified like clonal archetypal T-types I, II, and III. The rats presented lower serologic Toxoplasma gondii prevalence (8.8%) compared to contacting dogs (70.5%).

  7. Evaluation of Brucella abortus S19 vaccines commercialized in Brazil: immunogenicity, residual virulence and MLVA15 genotyping.

    Science.gov (United States)

    Miranda, Karina Leite; Poester, Fernando Padilla; Minharro, Silvia; Dorneles, Elaine Maria Seles; Stynen, Ana Paula Reinato; Lage, Andrey Pereira

    2013-06-24

    Live attenuated Brucella abortus S19 is the most effective vaccine against brucellosis in cattle. The assessment of the immunological parameters is essential to guarantee the biological quality of live anti-bacteria vaccines. The evaluation of genetic stability of live bacterial vaccines is also important in quality control. The aims of the present study were to compare (i) the immunogenicity and residual virulence, and (ii) the genotypic profile (MLVA15) of the eight S19 vaccines commercialized in Brazil to the USDA S19 reference strain. Two batches of each of the eight S19 commercial vaccines used in Brazil (A-H) were tested. They were submitted to the potency and residual virulence in vivo tests recommended by OIE and typed by the multiple-locus variable-number tandem repeat (VNTR) analysis (MLVA) described for Brucella spp. Our results demonstrated that all S19 vaccines commercialized in Brazil would be approved by Brazilian and OIE recommendations for potency and residual virulence. Furthermore, the S19 vaccine is genetically very homogeneous, as all but two batches (from the same manufacturer) tested showed identical MLVA15 profile. The two batches with different profiles presented six repeat units in locus Bruce07, instead of the five found in all other strains, including the USDA S19 reference strain. Although presenting a slightly different profile, this vaccine was also protective, as demonstrated by the immunogenicity and residual virulence assays performed. Therefore, the commercial Brazilian S19 vaccines were in accordance to Brazilian and international standards for immunogenicity and residual virulence tests. Moreover, our results also show that MLVA could be a useful inclusion to the list of in vitro tests required by the official control authorities to be applied to the commercial S19 vaccines, as an efficient assay to guarantee the quality and stability of the vaccine strains. PMID:23664986

  8. Prevalence and genotype distribution of human papillomavirus among Hakka women in China

    Science.gov (United States)

    Zeng, Xiang-Xing; Yan, Li-Xiang; Huang, Xiu-Xia; He, Cai-Hua; Liu, Wei-Guo; Yuan, Wen-Qing; Qiu, Yan-Ping

    2016-01-01

    Background Human papillomavirus (HPV) infection is the primary risk factor for cervical cancer. HPV genotypes are associated with varying degrees of pathogenicity. To better formulate strategies for cervical cancer prevention, we investigated the population-specific distribution of HPV genotypes, including those with high carcinogenicity. Methods From January to December 2012, a cervical cancer-screening program for HPV infection in Hakka women of Heyuan City Guangdong province was conducted. Of 736,000 women residents, 8,284 volunteers were recruited. The cytology specimens of 107 women were not adequate and excluded. Thus, 8,177 women submitted to polymerase chain reaction (PCR) sequencing of 16 HPV genotypes via MassARRAY spectrometry. Results Risk stratification based on genotypes indicated that the prevalence of overall, high-risk, and low-risk HPV infections was 12.27%, 14.20%, and 0.79%, respectively. Of the 1,003 women positively infected, 82.75% were infected with a single HPV type; 17.25% were infected with ≥2 types. Analysis revealed a U-shaped curve in HPV prevalence that correlated with age group, with peaks at ages 18–24 y (22.03%) and 60–65 y (25%). The most frequently detected HPV genotype was HPV-52 (26.81%), and then HPV-16 (17.54%), HPV-58 (14.25%), HPV-18 (10.16%), HPV-68 (8.27%), HPV-39 (5.68%), and HPV-51 (5.38%). Conclusions HPV-52 is the most prevalent genotype infecting Hakka women. Therefore, vaccination against HPV-52 is imperative. The prevalence of HPV infection is highest in the younger (18–24 y) and older (60–65 y) age groups, indicating that screening for HPV in Hakka women should be performed early and maintained in the elderly. PMID:27570770

  9. Distribution of Beijing Genotype Among Clinical Isolates of M. tuberculosis Circulating in Kazakhstan

    Directory of Open Access Journals (Sweden)

    Ainur Akhmetova

    2014-12-01

    Full Text Available Introduction. Methods of genotyping of M. tuberculosis play an important role in tuberсulosis (TB infection control. These techniques are used to detect or exclude laboratory errors, control recurrent cases, and determine ways of TB transmission. Today, there are more than 10 methods of genotyping; MIRU-VNTR is one of the most widely used methods in the world. In this study we aimed to estimate biological diversity of clinical isolates of M. tuberculosis from different regions of Kazakhstan based on MIRU-VNTR analysis.Materials and methods. MIRU-VNTR was used to genotype 134 clinical isolates of M. tuberculosis isolated from new cases and recurrent cases of TB from different regions of Kazakhstan.  Amplification was done using 15 MIRU-VNTR loci. Determination of the number of tandem repeats in the corresponding locus was performed via Quantity One v.4.4.0 (BioRad, USA software. H37Rv (NC_000962 reference strain was used as a positive control.Results. Phylogenic tree was built using www.miru-vntr.org web-resource based on the results of MIRU-VNTR analysis. Beijing family strains associated with drug resistance to antituberculosis drugs were prevalent among all isolates of M. tuberculosis circulating in Kazakhstan. Strains of the Beijing genotype were prevalent in both new cases (65% and recurrent cases (89.4% of tuberculosis. The second meaningful genotype that is spread in the territory of Kazakhstan is LAM, the frequency of distribution is 7.3% in new and 4.5% in recurrent cases. Other families of M. tuberculosis such as Ural, Haarlem, CAS, NEW-1, S were found in less than 4% of cases.Conclusion. Prevalence of Beijing family strains among all isolates of M. tuberculosis from different regions of Kazakhstan was shown. Strains of this family are prevalent among young people. This genotype is responsible for ongoing TB transmission in the present time. This genotype is more virulent; therefore, investigation of the epidemiology of the Beijing

  10. Distribution and Genotypic Analysis of Rubella Virus in West Java on 2011–2013

    Directory of Open Access Journals (Sweden)

    Acep T. Hardiana

    2015-03-01

    Full Text Available Rubella spreads around the world and dangerous especially for pregnant women because it can cause abortion, fetal death or congenital rubella syndrome (CRS almost 90% cases. Spread and identifiation of rubella genotypes in Indonesia is important to ensure the indigenous or importation virus. The purpose of this study was to determine the rubella genotype distribution and spread in West Java in effective prevention efforts. This study was conducted by examining the urine samples of suspect measles patients using WHO protocol through the virus isolation in vero cells, PCR, DNA sequencing, and analysis of the sequencing results. Samples taken from the measles-rubella surveillance program nationwide in 2011 ̶ 2013. Of the 251 urine samples were examined, 32 samples (12.7% were positive. A total of 28 cases (87.5% were genotype 1E while the remaining 4 cases (12.5% were genotype 2B. Rubella virus spread primarily occurs in District of Kuningan, Garut, Tasikmalaya, Bandung City, Cimahi City, and Tasikmalaya City. Prevention of the rubella diseases and CRS surveillance in endemic areas should be priority task to break the chain of transmission.

  11. Prevalence and genotype distribution of human papillomavirus infection in asymptomatic women in Liaoning province, China.

    Science.gov (United States)

    Xue, Hui; Lin, Xuyong; Li, Tianren; Yan, Xiaoxia; Guo, Kejun; Zhang, Yi

    2015-07-01

    Infection by human papillomavirus (HPV) is a necessary cause of cervical cancer. The purpose of this study was to investigate the prevalence and genotype distribution of HPV infection in Chinese women who were asymptomatic for cervical diseases. Cervical cytology samples were collected from 6479 asymptomatic Chinese women of Liaoning province, and tested for various HPV genotypes using a chip hybridization assay. HPV was found in 10.3% of all the asymptomatic women studied, with the prevalence of high risk HPV (HR HPV) and low risk HPV (LR HPV) being 9.5% and 1.1%, respectively. HPV genotypes 16, 52, and 58 were found the most frequently genotypes in the HR HPV positive women, and were present in 26.2%, 19.4% and 13.8%, respectively. A graph of HR HPV positive infection rates as a function of age is U-shaped, with a peak in women less than 30 years old and a second peak among women older than 50 years. Nearly half of the women infected with either HR HPV or LR HPV presented a normal looking cervix upon visual examination. The current study demonstrates that the epidemiology of HPV infection in asymptomatic Chinese women in Liaoning province is different from that in women from other regions, even from patients with cervical lesions in the same region. These findings could be used to guide the generation and design of an HPV vaccine for this population.

  12. Frequency distribution of hepatitis C virus genotypes in different geographical regions of Pakistan and their possible routes of transmission

    Directory of Open Access Journals (Sweden)

    Riazuddin Sheikh

    2008-05-01

    Full Text Available Abstract Background Information regarding hepatitis C virus genotypes and subtypes circulating in Pakistan and various risk factors for their transmission are not known well. The specific objective of this study was to find out the frequency of various HCV genotypes present in well-characterized Pakistani HCV isolates and their possible routes of transmission. Methods A total of 3351 serum samples were tested by type-specific genotyping assay. Out of 3351 HCV RNA positive patients, 2039 were males and 1312 were females. As regard as genotyped samples, 2165 belonged to Punjab region, 823 belonged to N.W.F.P., 239 to Sindh and 124 patients were from Balochistan. Results Out of the total 3351 tested serum samples, type-specific PCR fragments were observed in 3150 (94.00% serum samples. The distribution of genotypes of the typeable samples as determined by this assay, was as follows: 1664 (49.05% genotype 3a; 592 (17.66% genotype 3b; 280 (8.35% genotype 1a; 252 (7.52% genotype 2a; 101 (3.01% genotype 1b; 50 (1.49% with genotype 4; 25 (0.75% with 3c; 27 (0.80% genotype 2b; 6 (0.18% with subtype 5a; 5 (0.15% genotype 1c; 4 (0.12% with subtype 6a; 3 (0.09% genotype 2c; and 161 (4.80% patients were infected with mixed infection. Two hundred and one (5.99% serum samples were found untypeable by the present genotyping system. More than 86% and 72% patients with genotypes 3a and 3b respectively had received multiple injections in past. For genotypes 1a and 1b the route of transmission was major/minor surgery along with unknown reasons. Majority of the cases with type 2a, 2b and indeterminate genotypes were sporadic. Mixed infections were common in thalassaemic patients. Conclusion The most common HCV genotype in Pakistan is type 3a. Regional difference in genotypes was observed only in Balochistan province of Pakistan. More than 70% of the cases were acquired in hospitals through reuse of needles/syringes and major/minor surgery that is very common in this

  13. Helicobacter pylori vacA and cagA genotypes in patients from northeastern Brazil with upper gastrointestinal diseases

    Directory of Open Access Journals (Sweden)

    Meyssa Quezado de Figueiredo Cavalcante

    2012-06-01

    Full Text Available Helicobacter pylori causes chronic gastric inflammation and significantly increases the risk of duodenal and gastric ulcer disease and distal gastric carcinoma. In this study, we evaluated the Helicobacter pylori vacA and cagA genotypes in patients from a Brazilian region where there is a high prevalence of gastric cancer. Polymerase chain reaction (PCR was used to investigate vacA mosaicism and cagA status in the gastric mucosa of 134 H. pylori-positive patients, including 76 with gastritis: 28 with peptic ulcer disease and 30 with gastric cancer. The s1m1 variant was the predominant vacA genotype observed, whereas the s1 allele was more frequently observed in patients with more severe diseases associated with H. pylori infection [p = 0.03, odds ratio (OR = 5.72, 95% confidence interval (CI = 1.15-38.60]. Furthermore, all of the s1 alleles were s1b. Mixed vacA m1/m2 strains were found more frequently in patients with gastric cancer and a cagA-positive status was significantly associated with gastric cancer (p = 0.016, OR = 10.36, 95% CI = 1.35-217.31. Patients with gastric cancer (21/21, 100%, p = 0.006 or peptic ulcers (20/21, 95%, p = 0.02 were more frequently colonised by more virulent H. pylori strains compared to gastritis patients (41/61, 67.2%. In conclusion, in the northeastern of Brazil, which is one of the regions with the highest prevalence of gastric cancer in the country, infection with the most virulent H. pylori strains, carrying the cagA gene and s1m1 vacA alleles, predominates and is correlated with more severe H. pylori-associated diseases.

  14. MELTING CURVE ANALYSIS FOR THE SCREENING OF HEPATITIS B VIRUS GENOTYPES A, D AND F IN PATIENTS FROM A GENERAL HOSPITAL IN SOUTHERN BRAZIL

    Directory of Open Access Journals (Sweden)

    Carlos Eduardo BECKER

    2013-09-01

    Full Text Available Context Hepatitis B virus (HBV can cause fulminant hepatitis, cirrhosis and hepatocellular carcinoma, and is one of the most common causes of acute and chronic liver failure. The genetic variants of HBV can be decisive for the evolution of these diseases as well as for the election of therapy. Objectives The aim of this study was to evaluate and standardize an in house methodology based on the analysis of the melting curve polymerase chain reaction (PCR of real-time (qPCR to screen for genotypes A, D and F of HBV in patients from a hospital in Rio Grande do Sul, Brazil. Methods We evaluated 104 patients presumably with HBV chronic infection. Viral DNA was extracted from plasma and viral genotypes and different mutations were determined using PCR-based protocols. Results A PCR-based methodology was standardized for the analysis of genotypes A, D and F of HBV. The technique was based in a nested PCR with the final step consisting of a multiplex real-time PCR, using the melting curve as a tool for the differentiation of fragments. A higher frequency of genotype D (44.4%, followed by genotype A (22.2% and genotype F (3.7% was observed. Conclusion The standardized assay, a nested PCR-multiplex qPCR using specific primers, provides a rapid and accurate method for the differentiation of HBV genotypes that are more frequent in Southern Brazil – A, D and F. This method can be applied in the clinical practice.

  15. Prevalence, risk factors and genotypes of hepatitis B infection among HIV-infected patients in the State of MS, Central Brazil

    Directory of Open Access Journals (Sweden)

    Solange Zacalusni Freitas

    2014-09-01

    Full Text Available Objectives: A cross-sectional study on prevalence of HBV and HDV infection, risk factors and genotype distribution of HBV infection was conducted among 848 HIV-infected patients in Mato Grosso do Sul, Central Brazil. Methods: Serum samples of 848 participants were tested for hepatitis B surface antigen (HBsAg, hepatitis B core antibody (anti-HBc and hepatitis surface antibody (anti-HBs. HBsAg positive samples were tested for anti-HBc IgM, HBeAg, anti-HBe, anti-HCV, and total anti-HDV. HBsAg and anti-HBc positive were subjected to DNA extraction. Viral DNA was amplified by semi-nested PCR for the regions pre-S/S and then purified and genotyped/subgenotyped by direct sequencing. Student's t-test, chi-square test and Fisher's exact test were used to compare variables and to evaluate association between HBV positivity (defined as anti-HBc and/or HBsAg positivity and risk factors. Results: Among the 848 HIV infected patients investigated 222 had serological markers of HBV infection. The prevalence rate of HIV-HBV coinfection was 2.5% (21/848; 95% CI: 1.4–3.5%; 484 (57.1% patients were susceptible for HBV infection. There were no cases of anti-HDV positive and only one (0.1% anti-HCV-positive case among the HIV-HBV coinfected patients. Male gender, increasing age, family history of hepatitis, use of illicit drug, and homosexual activity were independent factors associated with HBV exposure. The phylogenetic analysis based on the S gene region revealed the presence of genotypes D (76.9%, F (15.4% and A (7.7% in the study sample. Conclusion: This study demonstrates the low prevalence of HIV-HBV infection and also highlights the need for early vaccination against HBV as well as testing for HBV, HCV and HDV in all HIV-infected individuals.

  16. Genotypes of Candida albicans involved in development of candidiasis and their distribution in oral cavity of non-candidiasis individuals.

    Science.gov (United States)

    Takagi, Yuki; Hattori, Hisao; Adachi, Hidesada; Takakura, Shunji; Horii, Toshinobu; Chindamporn, Ariya; Kitai, Hiroki; Tanaka, Reiko; Yaguchi, Takashi; Fukano, Hideo; Kawamoto, Fumihiko; Shimozato, Kazuo; Kanbe, Toshio

    2011-01-01

    Genotype characteristics and distribution of commensal Candida albicans should be studied to predict the development of candidiasis, however, extensive genotype analysis of commensal C. albicans has not been made. In this study, 508 C. albicans isolates were collected from patients with/without candidiasis and divided into 4 isolate groups (SG-1, oral cavity of non-candidiasis patients; SG-2, patients with cutaneous candidiasis; SG-3, patients with vaginal candidiasis; SG-4, patients with candidemia). These isolates were characterized to study the relationship between genotypes and pathogenicity using microsatellite analysis. Using CDC3 and CAI, 5 genotypes (I, 111: 115/33: 41; II, 115: 119/23: 23; III, 115: 123/18: 27; IV, 115: 123/33: 40; and V, 123: 127/32: 41) were found in 4.2%, 8.9%, 7.1%, 2.2% and 3.1% of the isolates, respectively. Genotypes II and III were commonly found in all isolate groups. These genotypes were further divided into 28 types by additional HIS3 and CAIII microsatellite markers. In this analysis, C. albicans with type 6 and type 23 was widely distributed as a commensal species in the oral cavity of non-candidiasis patients and found to be related with candidiasis development. Additionally, genotypes I and IV were found in SG-2 and/or SG-4, suggesting that the fungus with those genotypes is also involved in this development. In contrast, genotype V was not identified in any infective isolates.

  17. HPV genotype distribution in older Danish women undergoing surgery due to cervical cancer

    DEFF Research Database (Denmark)

    Hammer, Anne; Mejlgaard, Else; Gravitt, Patti;

    2015-01-01

    , Belgium) at the Department of Pathology, Aarhus University Hospital, Denmark. The main outcome was to estimate the age-specific prevalence of high-risk HPV genotypes included in the bivalent, the quadrivalent, and the nonavalent vaccine. RESULTS: Of 121 cases of cervical cancer included in this study, 113...... to increase with age (p = 0.1). The prevalence of HPV types included in the nonavalent vaccine was stable around 90% until the age of 75 years and then dropped to 63%. CONCLUSION: In the absence of waning immunity, the nonavalent HPV vaccine would be predicted to reduce cervical cancer burden in Denmark......INTRODUCTION: The prevalence of human papillomavirus (HPV)16/18 in cervical cancer may decrease with age. This study aimed to describe the HPV genotype distribution in Danish women aged 55 years or older with cervical cancer. MATERIAL AND METHODS: In this cross-sectional study we identified 153...

  18. In vitro culture of a novel genotype of Ehrlichia sp. from Brazil.

    Science.gov (United States)

    Zweygarth, E; Schöl, H; Lis, K; Cabezas Cruz, A; Thiel, C; Silaghi, C; Ribeiro, M F B; Passos, L M F

    2013-11-01

    Ehrlichiae are obligate intracytoplasmic Gram-negative, tick-borne bacteria belonging to the Anaplasmataceae family. Ehrlichioses are considered emerging diseases in both humans and animals. Several members of the genus Ehrlichia have been isolated and propagated in vitro. This study describes the continuous propagation of a Brazilian Ehrlichia sp. isolate in IDE8 tick cells, canine DH82 cells and bovine aorta cells. Initially, the organisms were isolated from the haemolymph of a Rhipicephalus (Boophilus) microplus tick into IDE8 cells. Infected IDE8 cells were brought from Brazil to Germany, where the organisms were continuously propagated in IDE8, DH82 and bovine aorta cells. Bovine aorta cells were infected and propagated for 3 months, corresponding to six subcultures, whereas the other two infected cell lines were kept for more than 1 year. During the cultivation period, 36 and 14 subcultures were carried out in IDE8 and DH82 cell cultures, respectively. Reinfection of IDE8 cells with organisms grown in DH82 cells was achieved. Sequence analysis made with a fragment of the 16S rRNA gene showed that this Ehrlicha sp. is closely related to Ehrlichia canis. However, the maximum likelihood phylogenetic tree shows that it falls in a separate phylogenetic clade from E. canis.

  19. Genotypic and phenotypic characterisation of enteroaggregative Escherichia coli from children in Rio de Janeiro, Brazil.

    Directory of Open Access Journals (Sweden)

    Fernanda L S França

    Full Text Available Enteroaggregative Escherichia coli (EAEC is a significant cause of diarrhoeal illness in both children and adults. Genetic heterogeneity and recovery of EAEC strains from both healthy and diseased individuals complicates our understanding of EAEC pathogenesis. We wished to establish if genetic or phenotypic attributes could be used to distinguish between strains asymptomatically colonising healthy individuals and those which cause disease. Genotypic screening of a collection of twenty four EAEC isolates from children with and without diarrhoea revealed no significant differences in the repertoire of putative virulence factors present in either group of strains. In contrast, EAEC strains from phylogroup A were more strongly associated with asymptomatic groups whereas strains from phylogroup D were more associated with cases of diarrhoea. Phenotypic screening revealed no differences in the ability of strains from either cohort of children to form biofilms, to adhere to and invade cells in tissue culture or to cause disease in the Caenorhabditis elegans model of infection. However, the latter assay did reveal significant reduction in nematode killing rates when specific virulence factors were deleted from human pathogenic strains. Our results suggest that current models of infection are not useful for distinguishing avirulent from pathogenic strains of EAEC but can be useful in studying the effect of specific virulence factors.

  20. Prevalence of hepatitis C virus among users attending a voluntary testing centre in Rio Grande, southern Brazil: predictive factors and hepatitis C virus genotypes.

    Science.gov (United States)

    Germano, F N; dos Santos, C A; Honscha, G; Strasburg, A; Gabbi, B; Mendoza-Sassi, R A; Soares, E A; Seuánez, H N; Soares, M A; Martínez, A M B

    2010-07-01

    We estimated the prevalence of hepatitis C (HCV) infection and associated risk factors in 750 individuals attending the Voluntary Counseling and Testing Center of Rio Grande (VCT/RG), in Southern Brazil, and identified viral genotypes. Demographic data and risk factors for HCV transmission were also collected and analysed. Anti-HCV antibody-positive individuals were tested for HCV-RNA and genotyped by sequencing the 5' untranslated region of the viral genome. Prevalence estimates of anti-HCV and HCV-RNA were 6% and 5.5%, respectively. We identified genotypes 1 (67%), 2 (2%) and 3 (31%); the latter was more prevalent than in other regions of Brazil. Anti-HCV prevalence in VCT/RG users was similar to previous reports. Age, previous blood transfusion, sexual orientation and injecting drug use were independent predictors of HCV infection. The presence of multiple risk factors was also associated with a higher risk for HCV infection. HCV genotype was not associated with any variable analysed in this study. PMID:20852195

  1. HPV prevalence and genotype distribution in a population-based split-sample study of well-screened women using CLART HPV2 human papillomavirus genotype microarray system

    DEFF Research Database (Denmark)

    Bonde, Jesper; Rebolj, Matejka; Ejegod, Ditte Møller;

    2014-01-01

    BACKGROUND: Human papillomavirus (HPV) genotyping assays are becoming increasingly attractive for use in mass screening, as they offer a possibility to integrate HPV screening with HPV vaccine monitoring, thereby generating a synergy between the two main modes of cervical cancer prevention...... furthermore updates the genotype distribution in Denmark prior to the inclusion of the HPV-vaccinated cohorts into the screening program, and as such represents a valuable baseline for future vaccine impact assessment........ The Genomica CLART HPV2 assay is a semi-automated PCR-based microarray assay detecting 35 high-risk and low-risk HPV genotypes. However, few reports have described this assay in cervical screening. An aim of the present study, Horizon, was to assess the prevalence of high-risk HPV infections in Copenhagen...

  2. Distribution of Cytomegalovirus Genotypes among Neonates Born to Infected Mothers in Islamabad, Pakistan

    Science.gov (United States)

    Mujtaba, Ghulam; Khurshid, Adnan; Sharif, Salmaan; Alam, Muhammad Masroor; Aamir, Uzma Bashir; Shaukat, Shahzad; Angez, Mehar; Rana, Muhammad Suleman; Umair, Massab; Shah, Aamer Ali; Zaidi, Syed Sohail Zahoor

    2016-01-01

    infection during pregnancy is a major cause of congenital CMV infection with comparable distribution of all three genotypes: gB, gN and gH in symptomatic and asymptomatic neonates. Our findings emphasize to conduct a comprehensive large scale survey and introduction of country wide routine screening at maternity clinics for early diagnosis of CMV to reduce its associated devastating outcomes. PMID:27367049

  3. Molecular characterization, distribution, and dynamics of hepatitis C virus genotypes in blood donors in Colombia.

    Science.gov (United States)

    Mora, Mónica Viviana Alvarado; Romano, Camila Malta; Gomes-Gouvêa, Michele Soares; Gutiérrez, Maria Fernanda; Carrilho, Flair José; Pinho, João Renato Rebello

    2010-11-01

    Hepatitis C virus (HCV) is a frequent cause of acute and chronic hepatitis and a leading cause for cirrhosis of the liver and hepatocellular carcinoma. HCV is classified in six major genotypes and more than 70 subtypes. In Colombian blood banks, serum samples were tested for anti-HCV antibodies using a third-generation ELISA. The aim of this study was to characterize the viral sequences in plasma of 184 volunteer blood donors who attended the "Banco Nacional de Sangre de la Cruz Roja Colombiana," Bogotá, Colombia. Three different HCV genomic regions were amplified by nested PCR. The first of these was a segment of 180 bp of the 5'UTR region to confirm the previous diagnosis by ELISA. From those that were positive to the 5'UTR region, two further segments were amplified for genotyping and subtyping by phylogenetic analysis: a segment of 380 bp from the NS5B region; and a segment of 391 bp from the E1 region. The distribution of HCV subtypes was: 1b (82.8%), 1a (5.7%), 2a (5.7%), 2b (2.8%), and 3a (2.8%). By applying Bayesian Markov chain Monte Carlo simulation, it was estimated that HCV-1b was introduced into Bogotá around 1950. Also, this subtype spread at an exponential rate between about 1970 to about 1990, after which transmission of HCV was reduced by anti-HCV testing of this population. Among Colombian blood donors, HCV genotype 1b is the most frequent genotype, especially in large urban conglomerates such as Bogotá, as is the case in other South American countries. PMID:20872715

  4. Indoor radon distribution in metropolitan region of Belo Horizonte, Brazil

    Energy Technology Data Exchange (ETDEWEB)

    Santos, Talita O.; Oliveira, Arno H. de, E-mail: talitaolsantos@yahoo.com.b, E-mail: arnoheeren@gmail.co [Universidade Federal de Minas Gerais (UFMG), Belo Horizonte, MG (Brazil). Dept. de Engenharia Nuclear; Rocha, Zildete; Barreto, Alberto A.; Souza, Leticia A.C. de; Miguel, Ronaldo A., E-mail: rochaz@cdtn.b, E-mail: aab@cdtn.b, E-mail: lets.coutinho@gmail.co, E-mail: ram@cdtn.b [Centro de Desenvolvimento da Tecnologia Nuclear (CDTN/CNEN-MG), Belo Horizonte, MG (Brazil)

    2009-07-01

    Human beings are exposed to ionizing radiation from many natural sources. Radon and its progeny have been recognized as the most important contributors to the natural radioactivity dose, accounting for about half of all human exposure to ionizing radiation. Radon ({sup 222}Rn) is a alpha-radioactive noble gas derived from the natural series of uranium (2{sup 38}U), which occurs in a wide concentration range in all geological materials, especially, in rocks, soils and waters. By diffusion and convection, radon migrates from the rocks and soils to atmosphere and through fissures, pipes and holes it may enter the dwellings and other buildings. Another important radon source in dwellings is its emanation from the construction material. The radon progeny concentration in dwellings has been receiving considerable global attention due to its potential effect in causing lung cancer if it deposited in upper respiratory tract when inhaled. This paper presents radon concentration distribution in dwellings in Metropolitan Region of Belo Horizonte - RMBH. The effective dose estimate is also presented for the RMBH inhabitants. The geological settings of the area are Archean rocks of Granitic Gnaissic Complex and of metasediments sequences of the great Precambrian unit of the Iron Quadrangle of Minas Gerais, Brazil. Radon concentration measurements were carried out with continuous detector AlphaGUARD PQ200PRO (Genitron), in passive mode and with passive detectors E-PERM{sup R} Eletret Ion Chamber-EIC. The radon progeny concentration was carried out with a solid state alpha spectroscope, the DOSEman PRO (Sarad). It was found an indoor radon concentration varying in a large range from 18.5 to 2671.4 Bq/m{sup -3}, with an average value of 148.0 Bqm{sup -3} and geometric mean equal to 128.2 Bqm{sup -3}. The variable results are due mainly to region geological factors and building material composition of dwellings. The equilibrium factor between radon and its progeny were determined

  5. A new species of Decimiana Uvarov (Insecta, Mantodea, Acanthopidae from Brazil, with remarks on the distribution of Decimiana bolivari (Chopard

    Directory of Open Access Journals (Sweden)

    Eliomar Menezes

    2012-11-01

    Full Text Available Decimiana Uvarov is a Neotropical genus of Mantodea with five South American species, three of them known from Brazil: Decimiana tessellata (Charpentier; Decimiana clavata Ippolito & Lombardo; and Decimiana bolivari (Chopard. A fourth species from Brazil is described and new records of Decimiana bolivari (Chopard from Brazil are presented and its distribution discussed.

  6. Α-Thalassemia: Genotypic Profile Associated with Ethnicity and Hematological Differentiation of Iron Deficiency Anemia in the Region of Uberaba, Minas Gerais, Brazil.

    Science.gov (United States)

    de Souza, Renata A Volpe; Carlos, Aline Menezes; de Souza, Bruna M Bereta; Rodrigues, Cibele Velloso; Pereira, Gilberto de Araujo; Moraes-Souza, Helio

    2015-01-01

    α-Thalassemia (α-thal) is a hereditary hemoglobinopathy characterized by microcytic anemia due to impaired production of α chains of human globin. Brazilian studies show that the most common genotype is an -α(3.7) deletion with the loss of one or two α genes. As the production of α chains is not as accentuated in these cases, the correct diagnosis can only be achieved through molecular analysis that is not usually routinely performed by laboratories. We investigated the occurrence of α-thal babies born between September 2011 to January 2013 at the hospital of the Universidade Federal do Triângulo Mineiro (UFTM), Uberaba, Brazil, and blood donors of the Uberaba Regional Blood Center, Hemominas Foundation, Uberaba, Brazil, correlating it with ethnicity and differences between hematological parameters of donors, α-thal and iron deficiency patients. α-Thalassemia was investigated for the most common deleted alleles (-α(3.7), -α(4.2), - -(SEA), - -(FIL), - -(THAI), -(α)(20.5) and - -(MED)). The incidence in newborns was 13.16% with a predominance of heterozygosity for the -α(3.7) genotype (12.35%), followed by the -α(3.7)/-α(3.7) (0.46%) and αα/-α(4.2) genotypes (0.35%). In blood donors, the prevalence of α-thal was 14.89%, with all cases being heterozygous for the -α(3.7) deletion. There was an association of the α-thal genotype with African ancestors for both groups, thereby confirming published data and showing the strong influence of Blacks on the composition of the population of Brazil's southeastern region. Minor changes were found between hematological parameters of blood donors with iron deficiency and α-thal that did not contribute to the differential diagnosis between the two types of anemia.

  7. Case Study: Tax reform, income distribution and poverty in Brazil: an applied general equilibrium analysis

    OpenAIRE

    Joaquim B de Souza Ferreira Filho; Carliton V dos Santos; Sandra M do Prado Lima

    2010-01-01

    This case study considers two frequently advocated approaches to reducing indirect taxation in Brazil: reduction in taxes on food; reduction in taxes on intermediate inputs to agriculture. To asses the effects of both on income distribution poverty levels, a bottom-up general equilibrium model of Brazil (TERM-BR) is linked to a microsimulation model. It is shown that one of the favoured policies is more poverty reducing, the other more income inequality reducing. Perhaps even more importantly...

  8. Cyanobacterial dominance in Brazil: Distribution and environmental preferences

    NARCIS (Netherlands)

    Soares, M.C.S.; Miranda, A.; Mello, M.M.; Roland, F.; Lurling, M.

    2013-01-01

    Based on a literature survey, we evaluated the periods of cyanobacterial dominance in Brazil. We hypothesized that variability of environmental forces along the country will promote or facilitate temporal and spatial mosaic in cyanobacterial dominance. The most striking outcomes are related to the d

  9. Hepatitis C virus (HCV) genotypes in 373 Italian children with HCV infection: changing distribution and correlation with clinical features and outcome

    OpenAIRE

    Bortolotti, F; Resti, M; Marcellini, M; Giacchino, R.; Verucchi, G.; Nebbia, G; Zancan, L; Marazzi, M G; Barbera, C; Maccabruni, A; Zuin, G.; Maggiore, G; Balli, F.; Vajro, P; Lepore, L

    2005-01-01

    Background and aim: Little is known of hepatitis C virus (HCV) genotypes in HCV infected children. This retrospective, multicentre study investigated genotype distribution and correlation with clinical features and outcome in a large series of Italian children.

  10. Myxomycetes in Alagoas state (Brazil and notes on its distribution

    Directory of Open Access Journals (Sweden)

    Andrea Carla Caldas Bezerra

    2014-09-01

    Full Text Available The state of Alagoas is located in the northeastern region of Brazil, and covers an area of 27,767 km2. Several different ecosystem types exist in this area, including mangroves forests and Atlantic rainforest on the coast, and Caatinga inland. The literature reports the occurrence of 43 species of Myxomycetes in Alagoas, all of which are recorded in humid forest environments, or in stored sugarcane bagasse. This study is the first one to record members of family Didymiaceae, and the genera Badhamia, Comatricha, Perichaena and Stemonaria in Alagoas. A species key is presented. Licea succulenticola, Reticularia jurana and Stemonaria longa are new references for the Caatinga biome. Licea succulenticola is also reported for the first time in Brazil.

  11. Potential Geographic Distribution of Hantavirus Reservoirs in Brazil

    OpenAIRE

    de Oliveira, Stefan Vilges; Escobar, Luis E.; Peterson, A. Townsend; Gurgel-Gonçalves, Rodrigo

    2013-01-01

    Hantavirus cardiopulmonary syndrome is an emerging zoonosis in Brazil. Human infections occur via inhalation of aerosolized viral particles from excreta of infected wild rodents. Necromys lasiurus and Oligoryzomys nigripes appear to be the main reservoirs of hantavirus in the Atlantic Forest and Cerrado biomes. We estimated and compared ecological niches of the two rodent species, and analyzed environmental factors influencing their occurrence, to understand the geography of hantavirus transm...

  12. Distribution of forensic marker allelic frequencies in Pernambuco, Northestern Brazil.

    Science.gov (United States)

    Santos, S M; Souza, C A; Rabelo, K C N; Souza, P R E; Moura, R R; Oliveira, T C; Crovella, S

    2015-01-01

    Pernambuco is one of the 27 federal units of Brazil, ranking seventh in the number of inhabitants. We examined the allele frequencies of 13 short tandem repeat loci (CFS1PO, D3S1358, D5S818, D7S820, D8S1179, D13S317, D16S539, D18S51, D21S11, FGA, TH01, vWA, and TPOX), the minimum recommended by the Federal Bureau of Investigation and commonly used in forensic genetics laboratories in Brazil, in a sample of 609 unrelated individuals from all geographic regions of Pernambuco. The allele frequencies ranged from 5 to 47.2%. No significant differences for any loci analyzed were observed compared with other publications in other various regions of Brazil. Most of the markers observed were in Hardy-Weinberg equilibrium. The occurrence of the allele 47.2 (locus FGA) and alleles 35.1 and 39 (locus D21S11), also described in a single study of the Brazilian population, was observed. The other forensic parameters analyzed (matching probability, power of discrimination, polymorphic information content, paternity exclusion, complement factor I, observed heterozygosity, expected heterozygosity) indicated that the studied markers are very informative for human forensic identification purposes in the Pernambuco population. PMID:25966202

  13. Essential Oil Variation from Twenty Two Genotypes of Citrus in Brazil-Chemometric Approach and Repellency Against Diaphorina citri Kuwayama.

    Science.gov (United States)

    Andrade, Moacir Dos Santos; Ribeiro, Leandro do Prado; Borgoni, Paulo Cesar; Silva, Maria Fátima das Graças Fernandes da; Forim, Moacir Rossi; Fernandes, João Batista; Vieira, Paulo Cezar; Vendramin, José Djair; Machado, Marcos Antônio

    2016-01-01

    The chemical composition of volatile oils from 22 genotypes of Citrus and related genera was poorly differentiated, but chemometric techniques have clarified the relationships between the 22 genotypes, and allowed us to understand their resistance to D. citri. The most convincing similarities include the synthesis of (Z)-β-ocimene and (E)-caryophyllene for all 11 genotypes of group A. Genotypes of group B are not uniformly characterized by essential oil compounds. When stimulated with odor sources of 22 genotypes in a Y-tube olfactometer D. citri preferentially entered the arm containing the volatile oils of Murraya paniculata, confirming orange jasmine as its best host. C. reticulata × C. sinensis was the least preferred genotype, and is characterized by the presence of phytol, (Z)-β-ocimene, and β-elemene, which were not found in the most preferred genotype. We speculate that these three compounds may act as a repellent, making these oils less attractive to D. citri. PMID:27338332

  14. Essential Oil Variation from Twenty Two Genotypes of Citrus in Brazil-Chemometric Approach and Repellency Against Diaphorina citri Kuwayama.

    Science.gov (United States)

    Andrade, Moacir Dos Santos; Ribeiro, Leandro do Prado; Borgoni, Paulo Cesar; Silva, Maria Fátima das Graças Fernandes da; Forim, Moacir Rossi; Fernandes, João Batista; Vieira, Paulo Cezar; Vendramin, José Djair; Machado, Marcos Antônio

    2016-01-01

    The chemical composition of volatile oils from 22 genotypes of Citrus and related genera was poorly differentiated, but chemometric techniques have clarified the relationships between the 22 genotypes, and allowed us to understand their resistance to D. citri. The most convincing similarities include the synthesis of (Z)-β-ocimene and (E)-caryophyllene for all 11 genotypes of group A. Genotypes of group B are not uniformly characterized by essential oil compounds. When stimulated with odor sources of 22 genotypes in a Y-tube olfactometer D. citri preferentially entered the arm containing the volatile oils of Murraya paniculata, confirming orange jasmine as its best host. C. reticulata × C. sinensis was the least preferred genotype, and is characterized by the presence of phytol, (Z)-β-ocimene, and β-elemene, which were not found in the most preferred genotype. We speculate that these three compounds may act as a repellent, making these oils less attractive to D. citri.

  15. New Toxoplasma gondii genotypes isolated from free-range chickens from the Fernando de Noronha, Brazil: unexpected findings

    Science.gov (United States)

    Worldwide comparison of Toxoplasma gondii isolates from free range chickens has indicated that T. gondii isolates from Brazil are phenotypically and genetically different than isolates from other countries; most strains from Brazil are pathogenic to mice, there is great genetic variability, most iso...

  16. Genotypes of Candida albicans isolated from healthy individuals and their distribution in patients with oral candidiasis.

    Science.gov (United States)

    Takagi, Yuki; Fukano, Hideo; Shimozato, Kazuo; Tanaka, Reiko; Horii, Toshinobu; Kawamoto, Fumihiko; Kanbe, Toshio

    2013-12-01

    For the study of Candida albicans genotypes involved in development of candidiasis, Candida albicans isolates were collected from healthy volunteers and patients with oral candidiasis and genotyped on the basis of 25S rDNA and microsatellite polymorphisms. In the microsatellite analysis using two microsatellite markers (CDC3 and CAI), 63 healthy volunteer isolates were classified into 35 genotypes (allelic relations to CDC3 alleles 1:2/CAI alleles 1:2), among which genotypes II (115:119/23:23), III (115:123/18:27), and V (123:127/32:41) were found at frequencies of 12.7%, 7.9%, and 7.9%, respectively. In 68 oral candidiasis isolates classified into 39 genotypes, genotypes II and III were identified in 4.4% and 20.6% of the isolates, respectively. The frequency of genotype III was higher in the candidiasis isolates than in the healthy isolates (p oral candidiasis.

  17. Geographic distribution of phlebotomine sandfly species (Diptera: Psychodidae in Central-West Brazil

    Directory of Open Access Journals (Sweden)

    Paulo Silva de Almeida

    2015-06-01

    Full Text Available This study updates the geographic distributions of phlebotomine species in Central-West Brazil and analyses the climatic factors associated with their occurrence. The data were obtained from the entomology services of the state departments of health in Central-West Brazil, scientific collections and a literature review of articles from 1962-2014. Ecological niche models were produced for sandfly species with more than 20 occurrences using the Maxent algorithm and eight climate variables. In all, 2,803 phlebotomine records for 127 species were analysed. Nyssomyia whitmani, Evandromyia lenti and Lutzomyia longipalpis were the species with the greatest number of records and were present in all the biomes in Central-West Brazil. The models, which were produced for 34 species, indicated that the Cerrado areas in the central and western regions of Central-West Brazil were climatically more suitable to sandflies. The variables with the greatest influence on the models were the temperature in the coldest months and the temperature seasonality. The results show that phlebotomine species in Central-West Brazil have different geographical distribution patterns and that climate conditions in essentially the entire region favour the occurrence of at least one Leishmania vector species, highlighting the need to maintain or intensify vector control and surveillance strategies.

  18. Geographic distribution of phlebotomine sandfly species (Diptera: Psychodidae) in Central-West Brazil

    Science.gov (United States)

    de Almeida, Paulo Silva; de Andrade, Andrey José; Sciamarelli, Alan; Raizer, Josué; Menegatti, Jaqueline Aparecida; Hermes, Sandra Cristina Negreli Moreira; de Carvalho, Maria do Socorro Laurentino; Gurgel-Gonçalves, Rodrigo

    2015-01-01

    This study updates the geographic distributions of phlebotomine species in Central-West Brazil and analyses the climatic factors associated with their occurrence. The data were obtained from the entomology services of the state departments of health in Central-West Brazil, scientific collections and a literature review of articles from 1962-2014. Ecological niche models were produced for sandfly species with more than 20 occurrences using the Maxent algorithm and eight climate variables. In all, 2,803 phlebotomine records for 127 species were analysed. Nyssomyia whitmani, Evandromyia lenti and Lutzomyia longipalpis were the species with the greatest number of records and were present in all the biomes in Central-West Brazil. The models, which were produced for 34 species, indicated that the Cerrado areas in the central and western regions of Central-West Brazil were climatically more suitable to sandflies. The variables with the greatest influence on the models were the temperature in the coldest months and the temperature seasonality. The results show that phlebotomine species in Central-West Brazil have different geographical distribution patterns and that climate conditions in essentially the entire region favour the occurrence of at least one Leishmania vector species, highlighting the need to maintain or intensify vector control and surveillance strategies. PMID:26018450

  19. Distribution of nuclear medicine service in Brazil; Distribuicao do servico de medicina nuclear no Brasil

    Energy Technology Data Exchange (ETDEWEB)

    Silva, Ana Carolina Costa da; Duarte, Alessandro; Santos, Bianca Maciel dos [Faculdade Metodo de Sao Paulo (FAMESP), Sao Paulo, SP (Brazil)

    2011-10-26

    The Brazil does not posses a good distribution of nuclear medicine service por all his territory. This paper shows the difference among country regions as far the number of clinics of nuclear medicine as is concerning, and also doctors licensed in the area and radioprotection supervisors, both licensed by the Brazilian Nuclear Energy Commission (CNEN)

  20. Dry season distribution of hydroids in a small tropical estuary, Pernambuco, Brazil

    NARCIS (Netherlands)

    Calder, D.R.; Maÿal, E.M.

    1998-01-01

    Hydroid distribution patterns along a horizontal ecocline in the Rio Formoso/Rio Ariquindá/Rio Porto Alegre system, a small and seasonally poikilohaline estuary on the tropical northeast coast of Brazil, were investigated. Collecting was undertaken during the dry season, in November 1993, by diving

  1. Brazil.

    Science.gov (United States)

    1985-09-01

    Brazil's population in 1985 was 135 million, with an annual growth rate (1982) of 2.3%. The infant mortality rate (1981) was 92/1000, and life expectancy stood at 62.8 years. 76% of the adult population was literate. Brazil is a federal republic which recognizes 5 political parties. 55% of the population is Portuguese, Italian, German, Japanese, African, or American Indian; 38% is white. Of the work force of 50 million, 35% are engaged in agriculture, 25% work in industry, and 40% are employed in services. Trade union membership totals 6 million. The agricultural sector accounts for 12% of the GDP and 40% of exports. Brazil is largely self-sufficient in terms of food. The GDP was US$218 billion in 1984, with an annual growth rate of 4%. Per capita GDP was US$1645. Brazil's power, transportation, and communications systems have improved greatly in recent years, providing a base for economic development. High inflation rates have been a persistent problem.

  2. FTO Genotype and 2-Year Change in Body Composition and Fat Distribution in Response to Weight-Loss Diets

    OpenAIRE

    Zhang, Xiaomin; Qi, Qibin; Zhang, Cuilin; Hu, Frank B.; Sacks, Frank M.; Qi, Lu

    2012-01-01

    Recent evidence suggests that the fat mass and obesity-associated gene (FTO) genotype may interact with dietary intakes in relation to adiposity. We tested the effect of FTO variant on weight loss in response to 2-year diet interventions. FTO rs1558902 was genotyped in 742 obese adults who were randomly assigned to one of four diets differing in the proportions of fat, protein, and carbohydrate. Body composition and fat distribution were measured by dual-energy x-ray absorptiometry and comput...

  3. Temporal changes in HCV genotype distribution in three different high risk populations in San Francisco, California

    Directory of Open Access Journals (Sweden)

    Evans Jennifer

    2011-08-01

    Full Text Available Abstract Background Hepatitis C virus (HCV genotype (GT has become an important measure in the diagnosis and monitoring of HCV infection treatment. In the United States (U.S. HCV GT 1 is reported as the most common infecting GT among chronically infected patients. In Europe, however, recent studies have suggested that the epidemiology of HCV GTs is changing. Methods We assessed HCV GT distribution in 460 patients from three HCV-infected high risk populations in San Francisco, and examined patterns by birth cohort to assess temporal trends. Multiple logistic regression was used to assess factors independently associated with GT 1 infection compared to other GTs (2, 3, and 4. Results Overall, GT 1 was predominant (72.4%, however younger injection drug users (IDU had a lower proportion of GT 1 infections (54.7% compared to older IDU and HIV-infected patients (80.5% and 76.6%, respectively. Analysis by birth cohort showed increasing proportions of non-GT 1 infections associated with year of birth: birth before 1970 was independently associated with higher adjusted odds of GT 1: AOR 2.03 (95% CI: 1.23, 3.34. African-Americans as compared to whites also had higher adjusted odds of GT 1 infection (AOR: 3.37; 95% CI: 1.89, 5.99. Conclusions Although, HCV GT 1 remains the most prevalent GT, especially among older groups, changes in GT distribution could have significant implications for how HCV might be controlled on a population level and treated on an individual level.

  4. Distribution of periphytic algae in wetlands (Palm swamps, Cerrado), Brazil.

    Science.gov (United States)

    Dunck, B; Nogueira, I S; Felisberto, S A

    2013-05-01

    The distribution of periphytic algae communities depends on various factors such as type of substrate, level of disturbance, nutrient availability and light. According to the prediction that impacts of anthropogenic activity provide changes in environmental characteristics, making impacted Palm swamps related to environmental changes such as deforestation and higher loads of nutrients via allochthonous, the hypothesis tested was: impacted Palm swamps have higher richness, density, biomass and biovolume of epiphytic algae. We evaluated the distribution and structure of epiphytic algae communities in 23 Palm swamps of Goiás State under different environmental impacts. The community structure attributes here analyzed were composition, richness, density, biomass and biovolume. This study revealed the importance of the environment on the distribution and structuration of algal communities, relating the higher values of richness, biomass and biovolume with impacted environments. Acidic waters and high concentration of silica were important factors in this study. Altogether 200 taxa were identified, and the zygnemaphycea was the group most representative in richness and biovolume, whereas the diatoms, in density of studied epiphyton. Impacted Palm swamps in agricultural area presented two indicator species, Gomphonema lagenula Kützing and Oedogonium sp, both related to mesotrophic to eutrophic conditions for total nitrogen concentrations of these environments.

  5. Distribution of HPV genotypes in cervical intraepithelial lesions and cervical cancer in Tanzanian women

    Directory of Open Access Journals (Sweden)

    Vidal Adriana C

    2011-11-01

    Full Text Available Abstract Background Infection with human papillomavirus (HPV is associated with uterine cervical intraepithelial neoplasia (CIN and invasive cancers (ICC. Approximately 80% of ICC cases are diagnosed in under-developed countries. Vaccine development relies on knowledge of HPV genotypes characteristic of LSIL, HSIL and cancer; however, these genotypes remain poorly characterized in many African countries. To contribute to the characterization of HPV genotypes in Northeastern Tanzania, we recruited 215 women from the Reproductive Health Clinic at Kilimanjaro Christian Medical Centre. Cervical scrapes and biopsies were obtained for cytology and HPV DNA detection. Results 79 out of 215 (36.7% enrolled participants tested positive for HPV DNA, with a large proportion being multiple infections (74%. The prevalence of HPV infection increased with lesion grade (14% in controls, 67% in CIN1 cases and 88% in CIN2-3. Among ICC cases, 89% had detectable HPV. Overall, 31 HPV genotypes were detected; the three most common HPV genotypes among ICC were HPV16, 35 and 45. In addition to these genotypes, co-infection with HPV18, 31, 33, 52, 58, 68 and 82 was found in 91% of ICC. Among women with CIN2-3, HPV53, 58 and 84/83 were the most common. HPV35, 45, 53/58/59 were the most common among CIN1 cases. Conclusions In women with no evidence of cytological abnormalities, the most prevalent genotypes were HPV58 with HPV16, 35, 52, 66 and 73 occurring equally. Although numerical constraints limit inference, findings that 91% of ICC harbor only a small number of HPV genotypes suggests that prevention efforts including vaccine development or adjuvant screening should focus on these genotypes.

  6. Studies on chaetognaths off Ubatuba region, Brazil: I. distribution and abundance

    OpenAIRE

    Liang Tsui Hua; Luz Amelia Vega-Pérez

    1994-01-01

    The distribution of chaetognath species off Ubatuba region, São Paulo State, Brazil, was studied during a program of multidisciplinary research. Ten species belonging to the genera Sagitta, Krohnita and Pterosagitta were identified. S. enflata was the dominant species followed by S.friderici and S. hispida. The species S. enflata, S. hispida, S. tenuis, S. bipunctata and JC pacifica were found in the Shelf water whereas S. serratodentata, S. minima, S. hexaptera and P. draco in the Tropical w...

  7. The diversity and distribution of Heliconia (Heliconiaceae) in Brazil A diversidade e distribuição de Heliconia (Heliconiaceae)

    OpenAIRE

    John Kress

    1990-01-01

    A review of the phylogenetic relationships of the Zingiberales is presented and the distribution patterns and diversity of Heliconia are discussed. Sixty-five species names have been applied to the heliconias that occur in Brazil. Of these 65 names, 28 are generally recognized synonyms. Of the remaining 37 species of Brazilian Heliconia, some controversy exists over the taxonomic status of at least eight. Two primary areas of distribution of species of Heliconia exist in Brazil: the Amazon ba...

  8. Distribution of hepatitis B virus genotypes in patients with chronic hepatitis B in Turkey

    Institute of Scientific and Technical Information of China (English)

    Mustafa Sunbul; Hakan Leblebicioglu

    2005-01-01

    AIM: Hepatitis B virus (HBV) strains isolated worldwide has been classified into eight genomic groups deduced from genome comparisons and designated as genotypes A to H. We aimed to investigate prevalence of HBV genotypes and subtypes in Turkey.METHODS: A total of 88 chronic hepatitis B (CHB) patients from 15 hospitals throughout the country were included.Patients who were HBsAg positive in serum at least for 6 mo, who had HBV-DNA in serum and elevation of ALT levels more than two times upper limit of normal, and who had percutaneous liver biopsy within 6 mo were included. Genotyping of HBV was done by restriction fragment length polymorphism (RFLP). The patients received subcutaneous 9 MU interferon-α 2a thrice a week for a period of 6 mo.RESULTS: Genotype D was detected in 78 of 88 (88.7%)patients, however, genotyping failed in two patients (2.3%),while no product was obtained in eight (9.0%) patients.Regarding subtypes, D2 was more prevalent (67 patients between 78% and 85.9%) followed by subtype D2+deletion (seven patients of 78 or 8.9%), subtype D1 (three patients of 78% or 3.9%) and subtype D3 (one patient of 78% or 1.3%). Thirty-three patients (37.5%) were HBeAg positive compared to 55 (62.5%) anti-HBe positive patients. The endpoint for the viral response of HBeAg positive patients was 27.2%, while it was found 52.7% in HBeAg negative patients (P<0.05). Long-term persistent viral response was 29.5% for all patients.CONCLUSION: This multi-center study indicates that the predominant genotype with CHB patients in Turkey like in other Mediterranean countries is genotype D.

  9. Phosphorus fractionation distribution in Guapimirim estuary: SE Brazil.

    Science.gov (United States)

    Vicente, Michel Arthur Faria; de Melo, Gustavo Vaz; Baptista Neto, José Antonio; de Oliveira, Allan Sandes

    2016-01-01

    The Guapimirim estuary is the main tributary of Guanabara bay and is located in the northeast portion. Although it is protected, this estuary has been experiencing strong anthropogenic pressure, which has led to changes in the natural characteristics. Large amounts of sewage are dumped into the bay through tributaries, thereby changing the water and bottom sediment quality. One of the main elements of sewage is phosphorus. Despite its importance to life, a high concentration of this nutrient in the environment can result in eutrophication. This work describes the phosphorus distribution in its different fractions in the bottom sediment at 16 stations located in the main channel of the Guapimirim estuary. These results are correlated with data on grain size, organic matter and calcium carbonate content in the bottom sediment and with physicochemical parameters of the bottom water. The grain size decreases toward the mouth of the estuary, whereas the organic matter and carbonate content increase. The salinity increases significantly at 3.5 km upstream from the mouth, where there is also a notable increase in fine sediments; the same site is the mean position of the salinity front. The temperature and pH increase in the same direction. The Pinorg-total ranges between 3.18 and 7.13 µmol g(-1), increasing toward the mouth. The same trend is observed for the other phosphorus fractions P-Ca, P-Fe and P-f.a., which range from 0.68 to 1.91, 0.79 to 1.71 and 0.03 to 0.93 µmol g(-1), respectively. The P-Ca and P-Fe fractions are the most representative in the Pinorg-total, occurring at 26.3 and 26.0 %, respectively. PMID:27610325

  10. Chronic Hepatitis C treatment for genotype 2 or 3 in Brazil: cost effectiveness analysis of peginterferon plus ribavirin as first choice treatment

    Directory of Open Access Journals (Sweden)

    Carine Raquel Blatt

    2014-04-01

    Full Text Available Brazilian Guidelines to HCV treatment (2007 recommended that the first choice treatment for patients with chronic hepatitis C (CHC and genotype 2 or 3 is interferon alpha (IFN plus ribavirin (RBV for 24 weeks. The aim of this study is compare the cost and effectiveness to Hepatitis C treatment in patients with genotype 2 or 3 of peginterferon alpha (PEG as the first choice of treatment within PEG for those that do not respond to IFN. The target population is CHC patients with genotype 2 or 3 in Brazil. The interventions are: PEG-SEC (first IFN plus RBV for 24 weeks, after, for non-responders and relapsers subsequently PEG plus RBV for 48 weeks; PEG-FIRST24 (PEG+RBV for 24 weeks. The type of the study is cost-effectiveness analysis. The data sources are: Effectiveness data from meta-analysis conducted on the Brazilian population. Treatment cost from Brazilian micro costing study is converted into USD (2010. The perspective is the Public Health System. The outcome measurements are Sustained Viral Response (SVR and costs. PEG-FIRST24 (SVR: 87.8%, costs: USD 8,338.27 was more effective and more costly than PEG-SEC (SVR: 79.2%, costs: USD 5,852.99. The sensitivity analyses are: When SVR rates with IFN was less than 30% PEG-FIRST is dominant. On the other hand, when SVR with IFN was more then 75% PEG-SEC is dominant (SVR=88.2% and costs USD $ 3,753.00. PEG-SEC is also dominant when SVR to PEG24 weeks was less than 54%. In the Brazilian context, PEG-FIRST is more effective and more expensive than PEG-SEC. PEG-SEC could be dominant when rates of IFN therapy are higher than 75% or rates of PEG24 therapy are lower than 54%.

  11. Genotype-Phenotype Correlations in CYP1B1-Associated Primary Congenital Glaucoma Patients Representing Two Large Cohorts from India and Brazil.

    Directory of Open Access Journals (Sweden)

    Mônica Barbosa de Melo

    Full Text Available Primary congenital glaucoma (PCG, occurs due to the developmental defects in the trabecular meshwork and anterior chamber angle in children. PCG exhibits genetic heterogeneity and the CYP1B1 gene has been widely implicated worldwide. Despite the diverse mutation spectra, the clinical implications of these mutations are yet unclear. The present study attempted to delineate the clinical profile of PCG in the background of CYP1B1 mutations from a large cohort of 901 subjects from India (n=601 and Brazil (n=300.Genotype-phenotype correlations was undertaken on clinically well characterized PCG cases from India (n=301 and Brazil (n=150 to assess the contributions of CYP1B1 mutation on a set of demographic and clinical parameters. The demographic (gender, and history of consanguinity and quantitative clinical (presenting intraocular pressure [IOP] and corneal diameter [CD] parameters were considered as binary and continuous variables, respectively, for PCG patients in the background of the overall mutation spectra and also with respect to the prevalent mutations in India (R368H and Brazil (4340delG. All these variables were fitted in a multivariate logistic regression model using the Akaike Information Criterion (AIC to estimate the adjusted odds ratio (OR using the R software (version 2.14.1.The overall mutation spectrum were similar across the Indian and Brazilian PCG cases, despite significantly higher number of homozygous mutations in the former (p=0.024 and compound heterozygous mutations in the later (p=0.012. A wide allelic heterogeneity was observed and only 6 mutations were infrequently shared between these two populations. The adjusted ORs for the binary (demographic and continuous (clinical variables did not indicate any susceptibility to the observed mutations (p>0.05.The present study demonstrated a lack of genotype-phenotype correlation of the demographic and clinical traits to CYP1B1 mutations in PCG at presentation. However, the

  12. Distribution of Acanthamoeba Genotypes Isolated from Recreational and Therapeutic Geothermal Water Sources in Southwestern Iran.

    Science.gov (United States)

    Niyyati, Maryam; Saberi, Reza; Latifi, Alireza; Lasjerdi, Zohreh

    2016-01-01

    A comprehensive survey was conducted along 10 km of geothermal rivers in southwestern Iran. A total of 40 water samples were tested for the presence of Acanthamoeba spp., and genotypes were determined by targeting the diagnostic fragment 3 region of the 18S rRNA gene. The pathogenic potential of all positive isolates was also identified using tolerance ability test. High occurrences of Acanthamoeba (50%) were detected in the sampling areas. Based on sequencing analysis, isolates belonging to T4 (93.7%) and T2 (6.25%) genotypes were reported. Thermo- and osmotolerance tests revealed that five strains are highly pathogenic. Since every collection site of this study was associated with high human activity, posting of warning signs, monitoring of recreational water sources, and awareness of high-risk people are of utmost importance. To the best of our knowledge, the present research is the first to report T2 genotype from geothermal water sources in Iran.

  13. Quantitative micro-PIXE comparison of elemental distribution in Ni-hyperaccumulating and non-accumulating genotypes of Senecio coronatus

    Science.gov (United States)

    Mesjasz-Przybyłowicz, J.; Przybyłowicz, W. J.; Prozesky, V. M.; Pineda, C. A.

    1997-07-01

    The Ni hyperaccumulator, plant species Senecio coronatus (Thunb.) Harv., Asteraceae is an example of plant adaptation mechanisms to different ecological conditions. This widespread species can inter alia be found on serpentine outcrops and the genotypes growing in serpentine soils show different ways of adaptation. The populations from two distant localities take up and translocate Ni in concentrations which are normally phytotoxic, while plants growing on a different site, in the vicinity of another hyperaccumulating species, absorb amounts which are typical for most of the plants found on serpentine soils. The NAC nuclear microprobe was used to compare the distribution of Ni and other elements in selected organs and cells with simultaneous use of PIXE and proton BackScattering (BS). Quantitative maps of stems showed large differences in concentrations and distributions of major and trace elements. In hyperaccumulating genotypes Ni is present everywhere within stem tissues, but the highest concentrations were found in the epidermis, cortex and phloem. In non-accumulating plants Ni was concentrated in the phloem. In the leaf epidermis Ni was concentrated in the cell walls for both accumulating and non-accumulating plants. These results suggest that biochemical diversity is more than morphological, because investigated genotypes belong to the same taxon.

  14. Correlation between genotypes of tRNA-linked short tandem repeats in Entamoeba nuttalli isolates and the geographical distribution of host rhesus macaques.

    Science.gov (United States)

    Feng, Meng; Komiyama, Tomoyoshi; Yanagi, Tetsuo; Cheng, Xunjia; Sherchand, Jeevan B; Tachibana, Hiroshi

    2014-01-01

    Several polymorphic markers, including serine-rich protein genes, have been used for the genotyping of isolates from the morphologically indistinguishable protozoan parasites Entamoeba histolytica, Entamoeba dispar, and Entamoeba nuttalli. Genotypes of tRNA-linked short tandem repeats (STRs) are highly polymorphic, but the correlation with geographical distribution is unknown. We have recently isolated 15 E. nuttalli strains from wild rhesus macaques in four locations in Kathmandu Valley, Nepal. The sequences of the serine-rich protein genes of the E. nuttalli strains differed among the four locations. In this study, we analyzed tRNA-linked STRs in six loci of the 15 strains. Two genotypes were found in loci N-K2, R-R, and S(TGA)-D, three in locus S-Q, and five in locus D-A. In locus A-L, one major genotype and ten minor genotypes were found, resulting in mixtures of two to six genotypes in eight strains. By combination of the main genotypes in the six loci, the 15 strains were divided into nine genotypes. The genotypes observed in E. nuttalli strains were quite different from those in E. histolytica and E. dispar. A phylogenetic tree constructed from tRNA-linked STRs in the six loci reflected the different places of isolation. These results suggest that sequence diversity of tRNA-linked STRs in E. nuttalli occurs with relatively high frequency and might be a marker of geographical distribution of host rhesus macaques, even in limited areas.

  15. Distribution of human papillomavirus (HPV genotypes and bacterial vaginosis presence in cervical samples from Paraguayan indigenous

    Directory of Open Access Journals (Sweden)

    Pamela Mongelos

    2015-10-01

    Conclusions: A large variety of HPV genotypes was detected and showed a slightly different pattern from previous studies on urban women in Paraguay, with the predominance of HR-HPV. Furthermore, the information of co-infections involved in BV could be useful for the improvement of national prevention programs, as well as for laboratory surveillance of these genital infections.

  16. Rotavirus genotype distribution during the pre-vaccine period in Bolivia: 2007–2008

    Science.gov (United States)

    Rivera, Rosario; Forney, Kristen; Castro, Maria René; Rebolledo, Paulina A.; Mamani, Nataniel; Patzi, Maritza; Halkyer, Percy; Leon, Juan S.; Iñiguez, Volga

    2013-01-01

    Summary Objectives Rotavirus is the most important etiology of severe diarrhea in Bolivia. The monovalent attenuated human oral rotavirus vaccine Rotarix® was introduced in Bolivia in 2008. We describe the molecular epidemiology of circulating rotavirus strains before vaccine introduction. Methods Two thousand one hundred thirty-five diarrheal samples were collected from hospitals in four Bolivian cities during 2007–2008. Forty-three percent (445 of 1030 rotavirus-positive samples) were analyzed for G and P genotypes. Among those, 331 were electropherotyped by polyacrylamide gel electrophoresis. Disease severity was quantified using a modified Vesikari scale. Results Among the 445 samples, five genotypes were found to be prevalent: G9P[8] (33%), G1P[6] (17%), G2P[4] (13%), G9P[6] (12%), and G1P[8] (4%). Co-infections with two or more strains accounted for 14% of samples. The most prevalent strain, G9, showed greater electropherotype diversity compared to other serogroups. Strain G1P[6] generally infected younger children and peaked later in the year than other strains. No particular genotype was associated with a higher severity score, though there was a significant difference in the duration of diarrhea between genotypes. Conclusions During the 2-year pre-vaccine period, substantial diversity of rotavirus co-circulating strains was observed. These data constitute a baseline against which changes in circulating strains post-vaccine introduction can be monitored. PMID:23688547

  17. Age-age correlation for early selection of rubber tree genotypes in São Paulo State, Brazil

    OpenAIRE

    Paulo de Souza Gonçalves; Nelson Bortoletto; Átila Bento Beleti Cardinal; Lígia Regina Lima Gouvêa; Reginaldo Brito da Costa; Mário Luiz Teixeira de Moraes

    2005-01-01

    In a field trial involving 68 rubber tree (Hevea spp.) clones calculation of genotypic correlation coefficients revealed significant age-age correlation from age 1 to 6 (immature period) for girth A and for age 7 to 12 (mature period) for girth B and for age 7 to 12 (production of latex) for yield. Rank correlation coefficients between all immature ages of girth (girth A), all ages of mature girth (girth B) and all annual rubber production (yield) were significant for the three traits, with t...

  18. Sentinel phenotype for rubella embryopathy: time-space distribution in Brazil

    Directory of Open Access Journals (Sweden)

    Iêda Maria Orioli

    2011-10-01

    Full Text Available The dyad comprising eye anomalies and congenital heart defects in the same newborn has been proposed as the best sentinel phenotype for the early detection of rubella embryopathy. Time-space birth prevalence distributions of the eye-heart dyad were described in 36 Brazilian hospitals from the Latin-American Collaborative Study of Congenital Anomalies - ECLAMC network, for the period 1994-2008. Seventy dyad cases observed among 554,531 births showed seasonal variation (Χ2 = 5.84; p < 0.05, suggesting an environmental etiology, with an increase in cases in October-March and acrophase in December. The secular distribution of dyad prevalence rates was consistent with the distribution of rubella cases in Brazil, showing a decrease from 1994 to 2004, followed by an increase until 2008. Two geographic clusters were identified, one with high and the other with low dyad prevalence. In the high prevalence cluster, a secular increase was observed, starting in 1999, matching the rubella epidemic waves observed in Brazil in 1998-2000 and 2006.

  19. Distribution, management and diversity of yam local varieties in Brazil: a study on Dioscorea alata L.

    Science.gov (United States)

    Siqueira, M V B M; Nascimento, W F; Silva, L R G; Ferreira, A B; Silva, E F; Ming, L C; Veasey, E A

    2014-02-01

    Widely spread in the tropics, yams were introduced into Brazil during the colonial period and are currently grown throughout the country. Despite its importance as a pharmacological and food source, there is a lack of studies describing how and where this tuber is grown in Brazil. The aim of this study was to provide an overview of the cultivation and distribution of Dioscorea alata in different Brazilian regions. A total of 63 farmers were visited in different municipalities and communities in four regions in the country: South, Southeast, Northeast and Midwest. Semi-structured interviews were conducted to collect socio-economic, use, management and diversity data for this crop. The majority of interviewers were men, married, with children, using retirement benefits and agriculture as income and family labour as the main support in the yam cultivation. A wide distribution of this species was found, with the occurrence of D. alata in the four sampled regions. A variety of vernacular names for this species was collected, differing according to the region where it is cultivated. Most farmers cultivate yams in fields, however an increased usage of home gardens for the cultivation of this tuber was found. Also, most farmers cultivate yams in association with other crops in areas of different sizes and slash and burn practices, although mostly disappearing, are still being used by many farmers. The results of this study provide more concrete data on the distribution and diversity of this important crop. PMID:25055086

  20. Rediscovery and geographic distribution of Philodryas agassizii (Jan, 1863 (Squamata: Dipsadidae in the state of Paraná, Southern Brazil

    Directory of Open Access Journals (Sweden)

    Gilberto Alves de Souza Filho

    2014-03-01

    Full Text Available Philodryas agassizii (Dipsadidae occurs in Brazil, Argentina, and Uruguay; in Brazil, it has been recorded in open areas of the regions Northeast, Central-West, Southeast and South. In the state of Paraná, it has only old records in southeastern of the state. We report, here, the rediscovery of P. agassizii in Paraná, forty years after the last record, and review its distribution within the state, including the central-south region.

  1. Rediscovery and geographic distribution of Philodryas agassizii (Jan, 1863) (Squamata: Dipsadidae) in the state of Paraná, Southern Brazil

    OpenAIRE

    Gilberto Alves de Souza Filho; Luciano Leandro Plombon

    2014-01-01

    Philodryas agassizii (Dipsadidae) occurs in Brazil, Argentina, and Uruguay; in Brazil, it has been recorded in open areas of the regions Northeast, Central-West, Southeast and South. In the state of Paraná, it has only old records in southeastern of the state. We report, here, the rediscovery of P. agassizii in Paraná, forty years after the last record, and review its distribution within the state, including the central-south region.

  2. Spatial distribution of stored grain insects in a rice storage and processing facility in Brazil

    OpenAIRE

    Lazzari, F. N.; Lazzari, F. A.; Lazzari, S. M. N.; Ceruti, F. C.

    2010-01-01

    This study describes the spatial distribution of stored product insects captured biweekly using foodbaited cage traps in a large rice storage and processing facility, in the state of Rio Grande do Sul, Brazil. Monitoring started in August 2009 and will be carried out for 1 year, the first 5 months of sampling being presented in this study. From end of August 2009 until the end of December 2009, a total of 9893 insects were captured in the 99 cage traps. The most abundant species were: Carpop...

  3. Hepatitis C genotype distribution and homology among geographically disparate injecting drug users in Afghanistan.

    Science.gov (United States)

    Sanders-Buell, Eric; Rutvisuttinunt, Wiriya; Todd, Catherine S; Nasir, Abdul; Bradfield, Andrea; Lei, Esther; Poltavee, Kultida; Savadsuk, Hathairat; Kim, Jerome H; Scott, Paul T; de Souza, Mark; Tovanabutra, Sodsai

    2013-07-01

    Hepatitis C virus (HCV) prevalence is high among injecting drug users in Afghanistan, but transmission dynamics are poorly understood. Samples from HCV-infected injecting drug users were sequenced to determine circulating genotypes and potential transmission linkages. Serum samples were obtained from injecting drug user participants in Hirat, Jalalabad, and Mazar-i-Sharif between 2006 and 2008 with reactive anti-HCV rapid tests. Specimens with detected HCV viremia were amplified and underwent sequence analysis. Of 113 samples evaluated, 25 samples (35.2%) were only typeable in NS5B, nine samples (12.7%) were only typeable in CE1, and 37 samples (52.1%) were genotyped in both regions. Of those with typeable HCV, all were Afghan males with a mean age of 31.1 (standard deviation [SD] ± 8.0) years and mean duration of injecting of 3.9 (SD ± 4.3) years. Most reported residence outside Afghanistan in the last decade (90.1%) and prior incarceration (76.8%). HCV genotypes detected were: 1a, (35.2%, n = 25), 3a (62.0%, n = 44), and 1b (2.8%, n = 2). Cluster formation was detected in NS5B and CE1 and were generally from within the same city. All participants within clusters reported being a refugee in Iran compared to 93.5% of those outside clusters. Only 22.2% (4/11) of those within clusters had been refugees in Pakistan and these four individuals had also been refugees in Iran. Predominance of genotype 3a and the association between HCV viremia and having been a refugee in Iran potentially reflects migration between Afghanistan and Iran among IDUs from Mazar-i-Sharif and Hirat and carry implications for harm reduction programs for this migratory population. PMID:23918535

  4. Distribution of multilocus genotypes of Escherichia coli within and between host families.

    OpenAIRE

    Caugant, D A; Levin, B. R.; Selander, R K

    1984-01-01

    Isolates from the intestinal Escherichia coli flora of 28 members of five families (including parents, children, and household pets) in Amherst, Massachusetts, and Rochester, New York, were characterized by the electrophoretic mobilities of 12 enzymes to estimate the extent of sharing of strains among associated and unassociated hosts. Among the 655 isolates examined, 60 different combinations of electromorphs (electrophoretic types or ETs), each representing a distinctive multilocus genotype...

  5. Hepatitis A Virus Genotype Distribution during a Decade of Universal Vaccination of Preadolescents

    Directory of Open Access Journals (Sweden)

    Lucía D’Andrea

    2015-03-01

    Full Text Available A universal vaccination program among preadolescents was implemented in Catalonia, Spain, during the period of 1999–2013 and its effectiveness has been clearly demonstrated by an overall significant attack rate reduction. However, reductions were not constant over time, and increases were again observed in 2002–2009 due to the occurrence of huge outbreaks. In the following years, in the absence of large outbreaks, the attack rate decreased again to very low levels. However, an increase of symptomatic cases in the <5 age group has recently been observed. This is an unexpected observation since children younger than 6 are mostly asymptomatic. Such a long vaccination campaign offers the opportunity to analyze not only the effectiveness of vaccination, but also the influence of the circulating genotypes on the incidence of hepatitis A among the different age groups. This study has revealed the emergence of genotype IC during a foodborne outbreak, the short-lived circulation of vaccine-escape variants isolated during an outbreak among the men-having-sex-with-men group, and the association of genotype IIIA with the increase of symptomatic cases among the very young. From a public health perspective, two conclusions may be drawn: vaccination is better at an early age, and the vaccination schedule must be complete and include all recommended vaccine doses.

  6. Genotype distribution of cervical human papillomavirus DNA in women with cervical lesions in Bioko, Equatorial Guinea

    Directory of Open Access Journals (Sweden)

    Carro-Campos Patricia

    2009-09-01

    Full Text Available Abstract Background The HVP vaccine is a useful tool for preventing cervical cancer. The purpose of this study is to determine the most frequent HPV genotypes in Equatorial Guinea in order to develop future vaccination strategies to apply in this country. Methods A campaign against cervical cancer was carried out in the area on a total of 1,680 women. 26 of the women, following cytological screening, were treated surgically with a loop electrosurgical excision procedure (LEEP. Cases were studied histologically and were genotyped from paraffin blocks by applying a commercial kit that recognized 35 HPV types. Results Cytological diagnoses included 17 HSIL, 1 LSIL, 5 ASC-H and 3 AGUS. Histological diagnosis resulted in 3 cases of microinvasive squamous cell carcinoma stage IA of FIGO, 9 CIN-3, 8 CIN-2, 2 CIN-1, 3 flat condylomas and mild dysplasia of the endocervical epithelium. Fifteen of twenty-five cases genotyped were positive for HPV (60%. HPV 16 and 33 were identified in four cases each, HPV 58 in two other cases, and HPV 18, 31, 52, and 82 in one case, with one HPV 16 and 58 coinfection. Conclusion The frequency of HPV types in the African area varies in comparison to other regions, particularly in Europe and USA. Vaccination against the five most common HPV types (16, 33, 58, 18, and 31 should be considered in the geographic region of West Africa and specifically in Equatorial Guinea.

  7. Distribution of Human Papillomavirus Genotypes among HIV-positive and HIV-negative Women in Cape Town, South Africa

    Directory of Open Access Journals (Sweden)

    Alicia C. McDonald

    2014-03-01

    Full Text Available Objective: HIV-positive women are known to be at high risk of Human Papillomavirus (HPV infection and its associated cervical pathology. Here we describe the prevalence and distribution of HPV genotypes among HIV-positive and negative women in South Africa, with and without cervical intraepithelial neoplasia (CIN. Methods: We report data on 1,371 HIV-positive women and 8,050 HIV-negative women, aged 17-65 years, recruited into three sequential studies in Cape Town, South Africa, conducted among women who had no history of cervical cancer screening recruited from the general population. All women were tested for HIV. Cervical samples were tested for high risk HPV DNA (Hybrid Capture 2 with positive samples tested to determine the specific genotype (Line Blot. CIN status was determined based on colposcopy and biopsy. Results: The HPV prevalence was higher among HIV-positive women (52.4% than among HIV-negative women (20.8% overall and in all age groups. Younger women, aged 17-19 years, had the highest HPV prevalence regardless of HIV status. HIV-positive women were more likely to have CIN 2 or 3 than HIV-negative women. HPV 16, 35, and 58 were the most common high-risk HPV types with no major differences in the type distribution by HIV status. HPV 18 was more common in older HIV-positive women (40-65 years with no or low grade disease, but less common in younger women (17-29 years with CIN 2 or 3, compared to HIV-negative counterparts (pConclusion: HIV-positive women were more likely to have high risk HPV than HIV-negative women but among those with HPV, the distribution of HPV types was similar by HIV status. Screening strategies incorporating HPV genotyping and vaccination should be effective in preventing cervical cancer in both HIV-positive and negative women living in sub-Saharan Africa.

  8. Global epidemiology of hepatitis C virus infection: An up-date of the distribution and circulation of hepatitis C virus genotypes

    Science.gov (United States)

    Petruzziello, Arnolfo; Marigliano, Samantha; Loquercio, Giovanna; Cozzolino, Anna; Cacciapuoti, Carmela

    2016-01-01

    AIM To review Hepatitis C virus (HCV) prevalence and genotypes distribution worldwide. METHODS We conducted a systematic study which represents one of the most comprehensive effort to quantify global HCV epidemiology, using the best available published data between 2000 and 2015 from 138 countries (about 90% of the global population), grouped in 20 geographical areas (with the exclusion of Oceania), as defined by the Global Burden of Diseases project (GBD). Countries for which we were unable to obtain HCV genotype prevalence data were excluded from calculations of regional proportions, although their populations were included in the total population size of each region when generating regional genotype prevalence estimates. RESULTS Total global HCV prevalence is estimated at 2.5% (177.5 million of HCV infected adults), ranging from 2.9% in Africa and 1.3% in Americas, with a global viraemic rate of 67% (118.9 million of HCV RNA positive cases), varying from 64.4% in Asia to 74.8% in Australasia. HCV genotype 1 is the most prevalent worldwide (49.1%), followed by genotype 3 (17.9%), 4 (16.8%) and 2 (11.0%). Genotypes 5 and 6 are responsible for the remaining < 5%. While genotypes 1 and 3 are common worldwide, the largest proportion of genotypes 4 and 5 is in lower-income countries. Although HCV genotypes 1 and 3 infections are the most prevalent globally (67.0% if considered together), other genotypes are found more commonly in lower-income countries where still account for a significant proportion of HCV cases. CONCLUSION A more precise knowledge of HCV genotype distribution will be helpful to best inform national healthcare models to improve access to new treatments. PMID:27678366

  9. Potential geographic distribution and conservation of Audubon's Shearwater, Puffinus lherminieri in Brazil

    Directory of Open Access Journals (Sweden)

    Ana Cecília P.A. Lopes

    2014-01-01

    Full Text Available Audubon's Shearwater (Puffinus lherminieri Lesson 1839 is a tropical seabird occurring mainly between southern Canada and the southeast coast of Brazil. Puffinus lherminieri is considered Critically Endangered on the Brazilian Red List because it only occurs in two known localities, both of which contain very small populations. However, many offshore islands along the Brazilian coast are poorly known and the discovery of new colonies would be of considerable significance for the conservation of this species. The aim of this study was to estimate the potential geographic distribution of Audubon's Shearwater in Brazil, based on ecological niche model (ENM using Maxent algorithm with layers obtained from AquaMaps environmental dataset. The ENM was based on 37 records for reproduction areas in North and South America. The model yielded a very broad potential distribution, covering most of the Atlantic coast ranging from Brazil to the US. When filtered for islands along the Brazilian coast, the model indicates higher levels of environmental suitability near the states of São Paulo, Rio de Janeiro, Espírito Santo and Bahia. However, P. lherminieri prefers islands in environments with warm saline water. Thus, based on the influence of currents that act on the Brazilian coast we can infer undiscovered colonies are most likely to occur on islands on coast of Bahia, Espírito Santo and extreme north of the Rio de Janeiro. These should be intensively surveyed while the islands south of Cabo Frio should be discarded. The existence of new populations would have profound effects on the conservation status of this enigmatic and rarely seen seabird.

  10. IS6110 restriction fragment length polymorphism of Mycobacterium tuberculosis isolated from patients with pulmonary tuberculosis in Campinas, Brazil: evidence of intercontinental distribution of strains

    Directory of Open Access Journals (Sweden)

    Ana Lucia Roscani Calusni

    2003-07-01

    Full Text Available Tuberculosis (TB is a major concern in developing countries. In Brazil, few genotyping studies have been conducted to verify the number of IS6110 copies present in local prevalent strains of Mycobacterium tuberculosis, the distribution and clustering of strains. IS6110 DNA fingerprinting was performed on a sample of M. tuberculosis isolates from patients with AFB smear-positive pulmonary TB, at a hospital in Brazil. The IS6110 profiles were analyzed and compared to a M. tuberculosis database of the Houston Tuberculosis Initiative, Houston, US. Seventy-six fingerprints were obtained from 98 patients. All M. tuberculosis strains had an IS6110 copy number between 5-21 allowing for differentiation of the isolates. Human immunodeficiency virus infection was confirmed in nearly half the patients of whom data was available. Fifty-eight strains had unique patterns, while 17 strains were grouped in 7 clusters (2 to 6 strains. When compared to the HTI database, 6 strains matched isolates from El Paso, Ciudad de Juarez, Houston, and New York. Recently acquired infections were documented in 19% of cases. The community transmission of infection is intense, since some clustered strains were recovered during the four-year study period. The intercontinental dissemination of M. tuberculosis strains is suspected by demonstration of identical fingerprints in a distant country.

  11. SPATIAL DISTRIBUTION OF PHLEBOTOMINAE IN PUERTO IGUAZU-MISIONES, ARGENTINA-BRAZIL-PARAGUAY BORDER AREA

    Directory of Open Access Journals (Sweden)

    Maria Soledad Santini

    2013-07-01

    Full Text Available SUMMARY The first Argentinian autochthonous human case of visceral leishmaniasis (VL was confirmed in Posadas (Misiones in 2006. Since then, the disease has increased its incidence and geographical distribution. In the 2006-2012 period, 107 human cases were detected (11 deaths. The presence of Lutzomyia longipalpis was detected in peridomiciles in Puerto Iguazú urban area in 2010; some of these findings were associated with households where cases of canine VL had already been reported. The objective of this study was to ascertain the abundance and spatial distribution of Lu. longipalpis in Puerto Iguazú City, on the Argentina-Brazil-Paraguay border. Lu. longipalpis proved to be exclusively urban and was found in 31% of the households sampled (n = 53, 67% of which belonged to areas of low abundance, 20% to areas of moderate abundance and 13% to areas of high abundance. Nyssomyia whitmani was the only species found both in urban and peri-urban environments, and Migonemyia migonei was registered only on the outskirts of the city. Due to the fact that Puerto Iguazú is considered to be at moderate risk at the moment, it is necessary to intensify human and canine case controls, as well as take integrated prevention and control measures regarding the environment, vectors and reservoirs on the Argentina-Brazil-Paraguay border area.

  12. Detection of Onchocerca volvulus (Nematoda: Onchocercidae infection in vectors from Amazonian Brazil following mass Mectizan™ distribution

    Directory of Open Access Journals (Sweden)

    Verônica Marchon-Silva

    2007-03-01

    Full Text Available Detection of Onchocerca volvulus in Simulium populations is of primary importance in the assessment of the effectiveness of onchocerciasis control programs. In Brazil, the main focus of onchocerciasis is in the Amazon region, in a Yanomami reserve. The main onchocerciasis control strategy in Brazil is the semi-annually mass distribution of the microfilaricide ivermectin. In accordance with the control strategy for the disease, polymerase chain reaction (PCR was applied in pools of simuliids from the area to detect the helminth infection in the vectors, as recommended by the Onchocerciasis Elimination Program for the Americas and the World Health Organization. Systematic sampling was performed monthly from September 1998 to October 1999, and a total of 4942 blackflies were collected from two sites (2576 from Balawaú and 2366 from Toototobi. The molecular methodology was found to be highly sensitive and specific for the detection of infected and/or infective blackflies in pools of 50 blackflies. The results from the material collected under field conditions showed that after the sixth cycle of distribution of ivermectin, the prevalence of infected blackflies with O. volvulus had decreased from 8.6 to 0.3% in Balawaú and from 4 to 0.1% in Toototobi.

  13. Adaptabilidade e estabilidade de genótipos de girassol no Brasil Central Adaptability and stability of sunflower genotypes in Central Brazil

    Directory of Open Access Journals (Sweden)

    Anna Karolina Grunvald

    2008-11-01

    Full Text Available O objetivo deste trabalho foi avaliar a adaptabilidade e estabilidade de genótipos de girassol, no Brasil Central, quanto ao rendimento de grãos e de óleo. Os dados foram obtidos de ensaios de genótipos de girassol coordenados pela Embrapa Soja, de 2004 a 2007. Foram usados os métodos de Eberhart & Russell, Porto et al., Rocha et al. e Annicchiarico. Foi realizada a decomposição do índice de recomendação de Annicchiarico nos ambientes favoráveis e desfavoráveis. Este método e o de Porto et al. foram similares e mais adequados para avaliar a adaptabilidade dos genótipos. Em relação ao rendimento de grãos, as variedades BRSGira 02 e Nutrissol apresentaram adaptação ampla. Os híbridos Agrobel 959, MG50 e V03005 e as variedades Catissol e Multissol apresentaram adaptação aos ambientes favoráveis, e os híbridos BRHS 02 e BRHS 04 apresentaram adaptação aos ambientes desfavoráveis. Quanto ao rendimento de óleo, os híbridos Agrobel 959, V03005, MG50, VDH 487, EXP 1441, EXP 1447 e EXP 1446 e as variedades BRSGira 01, BRSGira 02 e Nutrissol apresentaram adaptação ampla. O híbrido MG52 e as variedades Catissol e Multissol mostraram adaptação aos ambientes favoráveis, e BRHS 04 e BRHS 02 mostraram adaptação aos ambientes desfavoráveis. Os genótipos selecionados mostraram-se estáveis, mas em níveis diferenciados.The objective of this study was to evaluate the adaptability and stability of sunflower genotype grain and oil yield, in Central Brazil. The analyzed data were obtained from sunflower genotype trials coordinated by Embrapa Soja, from 2004 to 2007. Genotype adaptability and stability were evaluated using the methods of Eberhart & Russell, Porto et al., Rocha et al., and Annicchiarico. The Annicchiarico method divides sites into favorable and unfavorable environments. This method and the one of Porto et al. were similar and more adequate to evaluate genotypic adaptability. For grain yield, the varieties BRSGira

  14. Detection of porcine circovirus genotypes 2a and 2b in aborted foetuses from infected swine herds in the State of São Paulo, Brazil

    Directory of Open Access Journals (Sweden)

    de Castro Alessandra MMG

    2012-05-01

    Full Text Available Abstract Background Porcine circovirus type 2 (PCV2 has been associated with several disease complexes, including reproductive failure. The aim of this study was to identify the subtypes of PCV2 that are associated with reproductive failure in pigs from the State of São Paulo, Brazil and to investigate co-infections with other infectious organisms. Findings Samples of 168 aborted foetuses or mummified foetuses from five farrow-to-finish swine farms known to be infected with PCV2 and located in the State of São Paulo were tested for PCV2 by polymerase chain reaction (PCR. Positive samples were additionally tested for porcine parvovirus (PPV, Leptospira spp. and Brucella spp. by PCR. PCV2 was detected in 18 of the samples (10.7%. PPV, Brucella spp. and Leptospira spp were found in 2, 10 and 0 cases, respectively. Eleven PCV2 strains were sequenced and determined to be either genotype 2a (n = 1 or 2b (n = 10. Conclusions The findings indicate that the frequency of PCV2 infections in aborted porcine foetuses from the State of São Paulo is rather low (10.7% and that co-infection with other pathogens is common and may be involved in PCV2 associated reproductive failure. No repeatable, characteristic amino acid motifs for regions of the PCV2 capsid protein seemed to be associated with abortion in sows.

  15. Phenotypic and genotypic characteristics of shiga toxin-producing Escherichia coli strains isolated from children in São Paulo, Brazil

    Directory of Open Access Journals (Sweden)

    Beatriz EC Guth

    2002-12-01

    Full Text Available The biochemical and serological characteristics, virulence properties, and genetic relatedness of Shiga toxin-producing Escherichia coli (STEC strains isolated in São Paulo, from April 1989 through March 1990, were determined. This is also the first report on clinic findings of human STEC infections in Brazil. The only three STEC strains identified in that period were lysine decarboxylase negative, belonged to serotype O111ac: non-motile, were Stx1 producers, carried the eae and astA genes, and 2 of them also presented the EHEC-hly sequence. The children carrying STEC were all boys, with less than two years old, and had no previous history of hospitalization. None of them presented blood in stools. Vomiting, cough and coryza were the most common clinical manifestations observed. Although the STEC strains were isolated during summer months, and presented similar phenotypic and genotypic characteristics, carbohydrate fermentation patterns and PFGE analysis suggested that these diarrheal episodes were not caused by a single clone.

  16. Microspatial Distributional Patterns of Vectors of Cutaneous Leishmaniasis in Pernambuco, Northeastern Brazil

    Directory of Open Access Journals (Sweden)

    Maria Rita Donalisio

    2012-01-01

    Full Text Available The purpose of this study is to analyze the spatial distribution and population trends through time of Lutzomyia species in a long-term focus of cutaneous leishmaniasis transmission in an Atlantic Forest area, northeastern Brazil. Sand fly populations of different ecological niches were monitored spatiotemporally in 2009. To summarize vegetation characteristics and phenology, we calculated the Normalized Difference Vegetation Index from Landsat images. Using niche modeling approaches, we assessed suites of environmental factors to identify areas of transmission risk. Although 12 species were detected, L. whitmani was the most abundant and broadly distributed across the area, particularly in peridomiciliary locations, and associated negatively with denser vegetation areas. On the other hand, L. complexa, L. sordelli, and L. tupynambai were found almost exclusively in forested areas (<0.05, and associated positively with denser vegetation. Lutzomyia species' occurrences are related to specific environmental combinations (with contrast among species in the region.

  17. Spatial distribution of epibenthic molluscs on a sandstone reef in the Northeast of Brazil.

    Science.gov (United States)

    Martinez, A S; Mendes, L F; Leite, T S

    2012-05-01

    The present study investigated the distribution and abundance of epibenthic molluscs and their feeding habits associated to substrate features (coverage and rugosity) in a sandstone reef system in the Northeast of Brazil. Rugosity, low coral cover and high coverage of zoanthids and fleshy alga were the variables that influenced a low richness and high abundance of a few molluscan species in the reef habitat. The most abundant species were generalist carnivores, probably associated to a lesser offer and variability of resources in this type of reef system, when compared to the coral reefs. The results found in this study could reflect a normal characteristic of the molluscan community distribution in sandstone reefs, with low coral cover, or could indicate a degradation state of this habitat if it is compared to coral reefs, once that the significantly high coverage of fleshy alga has been recognized as a negative indicator of reef ecosystems health.

  18. Phlebotomine sand flies (Diptera, Psychodidae from Rio de Janeiro State, Brazil: species distribution and potential vectors of leishmaniases

    Directory of Open Access Journals (Sweden)

    Bruno Moreira Carvalho

    2014-03-01

    Full Text Available Phlebotomine sand flies (Diptera, Psychodidae from Rio de Janeiro State, Brazil: Species distribution and potential vectors of leishmaniases. Rio de Janeiro State, in Brazil, has endemic areas of both cutaneous and visceral leishmaniases. In these areas, entomologic surveillance actions are highly recommended by Brazil's Ministry of Health. The present work describes the results of sand fly captures performed by the Health Department of Rio de Janeiro State between 2009 and 2011 in several municipalities. An updated species list and distribution of phlebotomine sand flies in the state are provided based on an extensive literature review. Currently, the sand fly fauna of Rio de Janeiro State has 65 species, belonging to the genera Brumptomyia (8 spp. and Lutzomyia (57 spp.. Distribution maps of potential leishmaniases vector species Lutzomyia (Nyssomyia intermedia, L. migonei, L. (N. whitmani, L. (N. flaviscutellata and L. (Lutzomyia longipalpis are provided and their epidemiological importance is discussed.

  19. Frequency and genotypic distribution of GB virus C (GBV-C among Colombian population with Hepatitis B (HBV or Hepatitis C (HCV infection

    Directory of Open Access Journals (Sweden)

    Carrilho Flair J

    2011-07-01

    Full Text Available Abstract Background GB virus C (GBV-C is an enveloped positive-sense ssRNA virus belonging to the Flaviviridae family. Studies on the genetic variability of the GBV-C reveals the existence of six genotypes: genotype 1 predominates in West Africa, genotype 2 in Europe and America, genotype 3 in Asia, genotype 4 in Southwest Asia, genotype 5 in South Africa and genotype 6 in Indonesia. The aim of this study was to determine the frequency and genotypic distribution of GBV-C in the Colombian population. Methods Two groups were analyzed: i 408 Colombian blood donors infected with HCV (n = 250 and HBV (n = 158 from Bogotá and ii 99 indigenous people with HBV infection from Leticia, Amazonas. A fragment of 344 bp from the 5' untranslated region (5' UTR was amplified by nested RT PCR. Viral sequences were genotyped by phylogenetic analysis using reference sequences from each genotype obtained from GenBank (n = 160. Bayesian phylogenetic analyses were conducted using Markov chain Monte Carlo (MCMC approach to obtain the MCC tree using BEAST v.1.5.3. Results Among blood donors, from 158 HBsAg positive samples, eight 5.06% (n = 8 were positive for GBV-C and from 250 anti-HCV positive samples, 3.2%(n = 8 were positive for GBV-C. Also, 7.7% (n = 7 GBV-C positive samples were found among indigenous people from Leticia. A phylogenetic analysis revealed the presence of the following GBV-C genotypes among blood donors: 2a (41.6%, 1 (33.3%, 3 (16.6% and 2b (8.3%. All genotype 1 sequences were found in co-infection with HBV and 4/5 sequences genotype 2a were found in co-infection with HCV. All sequences from indigenous people from Leticia were classified as genotype 3. The presence of GBV-C infection was not correlated with the sex (p = 0.43, age (p = 0.38 or origin (p = 0.17. Conclusions It was found a high frequency of GBV-C genotype 1 and 2 in blood donors. The presence of genotype 3 in indigenous population was previously reported from Santa Marta region in

  20. History, distribution, and seasonal abundance of the Least Tern Sternula antillarum (Aves: Charadriiformes: Sternidae in Brazil

    Directory of Open Access Journals (Sweden)

    Caio J. Carlos

    2013-04-01

    Full Text Available We review existing data on the distribution of the Least Tern, Sternula antillarum along the Brazilian coast, based on the literature and museum specimens, and present results of a year-long study (October 2008 to September 2009 on the seasonal abundance of this species on a large tidal flat area, Cajuais Bank, in the State of Ceará, north-eastern Brazil. We evaluate whether the observed variation in the abundance of terns is due to the occurrence of an undocumented breeding colony, or alternatively, whether it results from an influx of migrants from the Northern Hemisphere. The recovery of historical data revealed that all literature references on the distribution of birds in the Americas, published up to the late 1990s include Brazil in the non-breeding range of the Least Tern. This inclusion is based on a few, old (late 19th and early 20th centuries museum specimens, all of which have been collected on the northern and north-eastern coasts of this country'. From the late 1980s, birds continued to be occasionally recorded along the coastline, running from the State of Amapá (01°N up to the State of Bahia (10°S, with records of single individuals in south-eastern and southern Brazil. An alleged record from Rocas Atoll, 260 km off the Brazilian mainland, might tentatively refer to the Old World Little Tern S. albifrons. At Cajuais Bank, Least Terns occurred from October 2008 to April 2009, and in September 2009. The highest numbers (> 800 individuals were recorded in January-February (Southern Hemisphere's summer. The species was observed in rather small numbers (< 30 in March-April (early-mid Southern Hemisphere fall and in September (early Southern Hemisphere spring, being absent from the area in May-August (Southern Hemisphere's fall and winter. In October-December 2008 (Southern Hemisphere's spring, and September 2009 (early Southern Hemisphere's summer, no birds were in breeding plumage. In January, about 32% of the birds were in

  1. Wilson's disease in Southern Brazil: genotype-phenotype correlation and description of two novel mutations in ATP7B gene

    Directory of Open Access Journals (Sweden)

    Ricardo Schmitt de Bem

    2013-08-01

    Full Text Available OBJECTIVE: Wilson's disease (WD is an inborn error of metabolism caused by abnormalities of the copper-transporting protein encoding gene ATP7B. In this study, we examined ATP7B for mutations in a group of patients living in southern Brazil. METHODS: 36 WD subjects were studied and classified according to their clinical and epidemiological data. In 23 subjects the ATP7B gene was analyzed. RESULTS: Fourteen distinct mutations were detected in at least one of the alleles. The c.3207C>A substitution at exon 14 was the most common mutation (allelic frequency=37.1% followed by the c.3402delC at exon 15 (allelic frequency=11.4%. The mutations c.2018-2030del13 at exon 7 and c.4093InsT at exon 20 are being reported for the first time. CONCLUSION: The c.3207C>A substitution at exon 14, was the most common mutation, with an allelic frequency of 37.1%. This mutation is the most common mutation described in Europe.

  2. Distribution and Abundance of Necrophagous Flies (Diptera: Calliphoridae and Sarcophagidae) in Maranhão, Northeastern Brazil

    OpenAIRE

    Pereira de Sousa, José Roberto; Carvalho-Filho, Fernando da Silva; Esposito, Maria Cristina

    2015-01-01

    This study aimed at surveying the local calliphorid and sarcophagid species in Maranhão State (Brazil) to determine their distribution and abundance, as well as the distribution of exotic Chrysomya species. In total, 18,128 calliphorid specimens were collected, distributed in 7 genera and 14 species. The species Hemilucilia semidiaphana (Rondani, 1850) and Paralucilia paraensis (Mello, 1969) were new state records. Chrysomya albiceps (Wiedemann, 1819) and Cochliomyia macellaria (F., 1775) wer...

  3. Phenotypic and genotypic variant of MDR-Mycobacterium tuberculosis multiple isolates in the same tuberculosis episode, Rio de Janeiro, Brazil

    Directory of Open Access Journals (Sweden)

    M.K.N. Andrade

    2009-05-01

    Full Text Available Assuming that the IS6110-restriction fragment length polymorphism (RFLP changes at a constant rate of 3.2 years, this methodology was applied to demonstrate, for the first time, variant patterns of Mycobacterium tuberculosis (MTB in multiple isolates obtained at short time intervals from sputum and blood of an HIV+ patient with multiple admissions to the Emergency Room and to the multidrug-resistant tuberculosis (MDR-TB Reference Center of a secondary-care hospital in Rio de Janeiro, Brazil. In sputum, the IS6110-RFLP appeared in isolates with two variant patterns with 10 and 13 IS6110 copies. However, blood presented only the pattern corresponding to 10 copies, suggesting compartmentalization. With regard to the exact match of 10 of 13 bands, this may be a subpopulation with the same clonal origin and this may be related to the IS6110 transposition. A susceptibility test demonstrated an MDR profile (INH R, RIF R, SM R, and EMB R, with the sputum isolate also exhibiting EMB S (R = resistant; S = sensitive. A gene mutation confirmed resistance only to streptomycin. There was agreement between the results of the phenotypic test and the clinical response to MDR-TB treatment, suggesting serious implications with regard to treatment administration based exclusively on molecular methods, and calling attention to the fact that more effective control strategies against the emergence of MDR strains must be implemented by the TB control program to prevent transmission of MDR-MTB strains at health facilities in areas highly endemic for TB.

  4. Epstein-Barr virus and human papillomavirus infections and genotype distribution in head and neck cancers.

    Directory of Open Access Journals (Sweden)

    Zeyi Deng

    Full Text Available To investigate the prevalence, genotypes, and prognostic values of Epstein-Barr virus (EBV and human papillomavirus (HPV infections in Japanese patients with different types of head and neck cancer (HNC.HPV and EBV DNA, EBV genotypes and LMP-1 variants, and HPV mRNA expression were detected by PCR from fresh-frozen HNC samples. HPV genotypes were determined by direct sequencing, and EBV encoded RNA (EBER was examined by in situ hybridization.Of the 209 HNC patients, 63 (30.1% had HPV infection, and HPV-16 was the most common subtype (86.9%. HPV E6/E7 mRNA expression was found in 23 of 60 (38.3% HPV DNA-positive cases detected. The site of highest prevalence of HPV was the oropharynx (45.9%. Among 146 (69.9% HNCs in which EBV DNA was identified, 107 (73.3% and 27 (18.5% contained types A and B, respectively, and 124 (84.9% showed the existence of del-LMP-1. However, only 13 (6.2% HNCs were positive for EBER, 12 (92.3% of which derived from the nasopharynx. Co-infection of HPV and EBER was found in only 1.0% of HNCs and 10.0% of NPCs. Kaplan-Meier survival analysis showed significantly better disease-specific and overall survival in the HPV DNA+/mRNA+ oropharyngeal squamous cell carcinoma (OPC patients than in the other OPC patients (P = 0.027 and 0.017, respectively. Multivariate analysis showed that stage T1-3 (P = 0.002 and HPV mRNA-positive status (P = 0.061 independently predicted better disease-specific survival. No significant difference in disease-specific survival was found between the EBER-positive and -negative NPC patients (P = 0.155.Our findings indicate that co-infection with HPV and EBV is rare in HNC. Oropharyngeal SCC with active HPV infection was related to a highly favorable outcome, while EBV status was not prognostic in the NPC cohort.

  5. H. pylori clinical isolates have diverse babAB genotype distributions over different topographic sites of stomach with correlation to clinical disease outcomes

    Directory of Open Access Journals (Sweden)

    Sheu Shew-Meei

    2012-05-01

    Full Text Available Abstract Background Intragenomic recombination between babA and babB mediates antigenic variations and may help H. pylori colonization. This study determined whether variable genotypes of babA and babB correlate to different clinical disease outcomes, and can distribute over the different gastric niches. Results This study enrolled 92 clinical strains (45 from peptic ulcer, 27 from gastritis, and 20 from gastric cancer to detect whether the babA and babB are at locus A or B by PCR reactions using the primers designed from the upstream and variable region of the babA and babB genes. Four genotypes of babA and babB (A B, AB B, A AB, AB AB were found. The distribution of the 4 genotypes in 92 clinical strains was significantly different among patients with different gastric diseases (p vs. 9.7%, p p p > 0.05. Besides, the study enrolled 19 patients to verify whether variable genotypes of babAB existed in the different gastric niches. Among the patients infected with more than one babAB genotypes over antrum and corpus, there were higher rate of genotypes as A B or AB AB in isolates from antrum than in those from corpus (75.0 % vs. 16.7%, p  Conclusions The H. pylori isolate with the AB AB genotype correlates with an increased gastric cancer risk, and colonize in an antrum predominant manner.

  6. Anatomical distribution of Mycobacterium bovis genotypes in experimentally infected white-tailed deer.

    Science.gov (United States)

    Thacker, Tyler C; Palmer, Mitchell V; Robbe-Austerman, Suelee; Stuber, Tod P; Waters, W Ray

    2015-10-22

    Mycobacterium bovis (M. bovis) causes tuberculosis in white-tailed deer (WTD). Natural infection of WTD with M. bovis is most closely mimicked by instilling inoculum into palatine tonsillar crypts. One hundred fifty days after intratonsillar inoculation, M. bovis was cultured from 30 tissues originating from 14 deer. Whole-genome sequencing (WGS) was performed on the original inoculum, single colonies subcultured from the original inoculum, and M. bovis isolated from each culture positive tissue. Single nucleotide polymorphisms (SNP) were identified by comparing the derived sequences to the reference strain AF2122/97. Results indicate that the majority of the SNPs that were identified were homogeneous between the inoculum and the isolates from the tissues. The majority of individual tissues had different WGS genotypes from each other, suggesting that dissemination of M. bovis beyond the initial site of infection may require few mycobacteria representing a bottleneck. PMID:26243696

  7. Distribution of multilocus genotypes of Escherichia coli within and between host families.

    Science.gov (United States)

    Caugant, D A; Levin, B R; Selander, R K

    1984-06-01

    Isolates from the intestinal Escherichia coli flora of 28 members of five families (including parents, children, and household pets) in Amherst, Massachusetts, and Rochester, New York, were characterized by the electrophoretic mobilities of 12 enzymes to estimate the extent of sharing of strains among associated and unassociated hosts. Among the 655 isolates examined, 60 different combinations of electromorphs (electrophoretic types or ETs), each representing a distinctive multilocus genotype, were identified, of which 85% were recovered from only a single individual. On average, 11% of the ETs isolated from the same family were shared by two or more members; 4.9% of ETs were shared among members of unassociated families living in the same city; and only 2% were shared by families in different cities. All three ETs that were recovered from multiple hosts in the present study are widespread clones that have been isolated from many other host individuals in North America and Sweden. PMID:6376625

  8. [Trends and spatial distribution of mortality from external causes in Salvador, Bahia State, Brazil].

    Science.gov (United States)

    de Freitas, E D; Paim, J S; da Silva, L M; Costa, M da C

    2000-01-01

    Since 1980, external causes (ICD-9 E800-E999) have been ranked as the second leading causal group for mortality in Brazil, thus becoming a major public health problem. This study aimed to describe spatial distribution trends for violent deaths in the urban setting of Salvador, a city in Northeast Brazil, for the years 1988, 1991, and 1994. An ecological study was conducted, and mortality data were obtained from death certificates and the archives of the Institute for Forensic Medicine. There was an increase of 34.6% in the number of deaths from external causes between 1988 and 1994. The highest mortality rates were among men from 20 to 29 years of age (from 192.0 to 262.0/100,000) and those 65 years and over (from 188.7 to 258.1/100,000). Homicides were the leading cause of violent deaths in about 75.0% of neighborhoods. The authors discuss the need for comprehensive public policies and an interdisciplinary approach to elucidate the causes and deal with the problem of violence. PMID:11175529

  9. 3D elemental distribution images by XRF{mu}CT at LNLS-Brazil

    Energy Technology Data Exchange (ETDEWEB)

    Pereira, G.R.; Rocha, H.S.; Calza, C. [Nuclear Instrumentation Laboratory (LIN), COPPE, UFRJ, P.O. Box 68509, 21945-970 Rio de Janeiro (Brazil); Anjos, M.J. [Physics Institute, UERJ (Brazil); Lima, I. [Nuclear Instrumentation Laboratory (LIN), COPPE, UFRJ, P.O. Box 68509, 21945-970 Rio de Janeiro (Brazil); University Department of Mechanical Engineering and Energy, UERJ (Brazil); Perez, C.A. [Brazilian Synchrotron Light Laboratory (Brazil); Lopes, R.T., E-mail: ricardo@lin.ufrj.br [Nuclear Instrumentation Laboratory (LIN), COPPE, UFRJ, P.O. Box 68509, 21945-970 Rio de Janeiro (Brazil)

    2011-10-01

    An X-ray Transmission Microtomography (CT) system combined with an X-ray Fluorescence Microtomography (XRF{mu}CT) system was implemented in the Brazilian Synchrotron Light Laboratory (LNLS), Campinas, Brazil. The main objective of this work is to determine the elemental distribution in biological samples (breast, prostate and lung samples) in order to verify the concentration of some elements correlated with characteristics and pathology of each tissue observed by the transmission CT. The experiments were performed at the X-Ray Fluorescence beamline (D09B-XRF) of the Brazilian Synchrotron Light Laboratory, Campinas, Brazil. A quasi-monochromatic beam produced by a multilayer monochromator was used as an incident beam. The sample was placed on a high precision goniometer and translation stages that allow its rotation as well as translation perpendicular to the beam. The fluorescence photons were collected with an energy dispersive HPGe detector placed at 90{sup o} to the incident beam, while transmitted photons were detected with a fast Na(Tl) scintillation counter placed behind the sample on the beam direction. The CT images were reconstructed using a filtered back-projection algorithm and the XRF{mu}CT were reconstructed using a filtered back-projection algorithm with absorption corrections. The 3D images were reconstructed using the 3D-DOCTOR software.

  10. Schistosomal glomerulopathy and changes in the distribution of histological patterns of glomerular diseases in Bahia, Brazil

    Directory of Open Access Journals (Sweden)

    Washington Luis Conrado dos-Santos

    2011-11-01

    Full Text Available Distinct patterns of glomerular lesions, including membranoproliferative glomerulonephritis and focal segmental glomerulosclerosis, are associated with infection by Schistosoma mansoni or Schistosoma japonicum. Evidence suggests that immune complex deposition is the main mechanism underlying the different forms of schistosomal glomerulonephritis and that immune complex deposition may be intensified by portal hypertension. The relationship between focal segmental glomerulosclerosis and schistosomiasis remains poorly understood. A clinicopathologic classification of schistosomal glomerulopathies was proposed in 1992 by the African Association of Nephrology. In Brazil, mass treatment with oral medications has led to a decrease in the occurrence of schistosomal glomerulopathy. In a survey of renal biopsies performed in Salvador, Brazil, from 2003-2009, only 24 (4% patients were identified as positive for S. mansoni infection. Among these patients, only one had the hepatosplenic form of the disease. Focal segmental glomerulosclerosis was found in seven patients and membranoproliferative glomerulonephritis was found in four patients. Although retrospective studies on the prevalence of renal diseases based on kidney biopsies may be influenced by many patient selection biases, a change in the distribution of glomerulopathies associated with nephrotic syndrome was observed along with a decline in the occurrence of severe forms of schistosomiasis.

  11. Studies on radioactivity distribution and radioactive mineral identification in uranium ores from Espinharas (PB), Brazil

    International Nuclear Information System (INIS)

    Studies about the identification of radioactive minerals in uranium bearing rocks from Espinharas (PB), Brazil are presented. Autoradiography with α-sensitive nuclear emulsions was utilized for determining radioctivity distributions and for localizing radioactive minerals, in combination with microscopy, X-ray diffractometry, PIXE and eletron microprobe analysis for its identification. Mineralized gneisse and feldspatic rock, the two principal samples studied, show distinct differences in radioactive distribution patterns, however the main carriers for U and Th seem to be the same. Microanalysis shows that elements are associated with Si, Ca, Fe and Al an some trace elements like Y, Zr, Ti, etc. U and Th are distributed uniformly in feldspatic rock and inhomogeneously in mineralized gneisse, indicating that the zonary structure of the radioactive cristals, frequently observed in gneisse, could be due to variable U:Th ratios. Chemical analysis, X-ray diffraction datas and microscopic studies indicates that the principal carrier for radioactivity in the rocks of Espinharas is a silicate mineral of U and Th, probably situaded in the series of transition: Coffinite -> uraninite, thorogummite -> thorianite. Some additional experiments about leachability of uranium with diluted sulfuric acid are reported, which confirm the different nature of radioactivity distribution in feldspatic and gneissic rocks. (author)

  12. Variations in Concentration and Distribution of Health-Related Elements Affected by Environmental and Genotypic Differences in Rice Grains

    Institute of Scientific and Technical Information of China (English)

    REN Xue-liang; LIU Qing-long; WU Dian-xing; SHU Qing-yao

    2006-01-01

    A research work was conducted to investigate the variations in concentration and distribution of health-related elements affected by environmental and genotypic differences in rice grains. The grain of Xieqingzao B (indica rice variety) and Xiushui 110 (japonica rice variety) were divided into: hull, bran and milled rice, based on the conventional rice consumption and process. Xieqingzao B was grown at four different locations, and at one location, it was planted in the same field and season as Xiushui 110. In addition, another four indica and four japonica varieties were cultivated in the same field and time to analyze the elements in milled rice. The average concentrations of total P and phytic acid P were the highest in the bran, followed by milled rice and hull; Zn, K, Mg, and As concentrations were the highest in bran, followed by hull and milled rice, while Fe, Ca, and Cu concentrations were the highest in the hull, but similar in bran and milled rice. The result indicated that genotype and environment significantly affected the concentrations of all the tested elements, while the distribution of the above elements in grains was not in the same order as concentration. Moreover, all the elements except 97.7% of Cu and 93.2% of Fe was deposited in the hull on average, were mostly distributed either in the bran (37.3% and 57.7% for K and phytic acid P) or in milled rice (41.7%, 42.6%, 40.3%, 49.8% for Zn, Mg, As, total P, respectively).

  13. Genotypic Difference of Plants in K—Enrichment Capability and the Distribution of K in Plant Rhizosphere

    Institute of Scientific and Technical Information of China (English)

    YANWEIDONG; SHIWEIMNG; 等

    1997-01-01

    Plant genotypic difference of potassium-enrichment capalbility and potassium(K) distribution at root-soil interace of different plant genotypes were studied by using seven plant species and eight varieties of tobacco(Nicotiana tabacum L.) The results indicated that K enrichment capability was: Ethiopian guizotia(Guizotia abyssinica Cass.)>feather cockscomb(Celosia argentea L.)>alligator alternanthera(Alternathera philoxeroides (Mart.) Griseb.)>tobacco>sesbania(Sesbania cannabina(Retz.)Pers.)>wheat(Triticum aestivum L.)>broadbean(Vicia faba L.).Ethiopian guizotia showed very high K-enrichment capability at different soil K levles,and the K content in its dry matter was over 110 mg kg-1 when soil K was fully supplied ,and about 60 mg kg-1 when no K fertilizer was applied.For alligator alternanthera,the capabiltiy to accumulate K was closely related with its growth medium,When it was grown on soils ,both the K content and K uptake rate of the plant were similar to whose of tobacco.Evident K depletion was observed in the rhizospere of all plant species,and the depletion rate was related to the capability of enrichment of plant .

  14. Changes in Test Scores Distribution for Students of the Fourth Grade in Brazil: A Relative Distribution Analysis for the Years 1997-2005

    Science.gov (United States)

    Rodrigues, Clarissa Guimaraes; Rios-Neto, Eduardo Luiz Goncalves; de Xavier Pinto, Cristine Campos

    2013-01-01

    In Brazil, the mean of math test scores for students of the fourth grade declined by approximately 0.2 standard deviation in the late 1990s. However, the potential changes in the distribution of scores have never been addressed. It is unclear if the decline was caused by deterioration in student performance levels at the upper and/or lower tails…

  15. Evidence for the Gompertz curve in the income distribution of Brazil 1978-2005

    Science.gov (United States)

    Moura, N. J., Jr.; Ribeiro, M. B.

    2009-01-01

    This work presents an empirical study of the evolution of the personal income distribution in Brazil. Yearly samples available from 1978 to 2005 were studied and evidence was found that the complementary cumulative distribution of personal income for 99% of the economically less favorable population is well represented by a Gompertz curve of the form G(x) = exp [exp (A-Bx)], where x is the normalized individual income. The complementary cumulative distribution of the remaining 1% richest part of the population is well represented by a Pareto power law distribution P(x) = βx-α. This result means that similarly to other countries, Brazil’s income distribution is characterized by a well defined two class system. The parameters A, B, α, β were determined by a mixture of boundary conditions, normalization and fitting methods for every year in the time span of this study. Since the Gompertz curve is characteristic of growth models, its presence here suggests that these patterns in income distribution could be a consequence of the growth dynamics of the underlying economic system. In addition, we found out that the percentage share of both the Gompertzian and Paretian components relative to the total income shows an approximate cycling pattern with periods of about 4 years and whose maximum and minimum peaks in each component alternate at about every 2 years. This finding suggests that the growth dynamics of Brazil’s economic system might possibly follow a Goodwin-type class model dynamics based on the application of the Lotka-Volterra equation to economic growth and cycle.

  16. A Unique Pattern of HCV Genotype Distribution on Hainan Island in China Revealed by Evolutionary Analysis

    Directory of Open Access Journals (Sweden)

    Tao Wu

    2016-06-01

    Full Text Available Background/Aims: Different genotypes of HCV may differ in both disease progression and response to antiviral therapies. Hainan Island has been inhabited by the “Li” aboriginal minority for centuries. We aimed to provide a better understanding of HCV infection on Hainan Island, so that the information would help improve strategies for HCV prevention and control on the island and in the wider country. Methods: Using RT-PCR and DNA sequencing, we determined HCV sequences from 100 patients living on Hainan Island. Results: Phylogenetic analysis classified these sequences into six subtypes: 6a (n=35, 1b (n=31, 3b (n=16, 2a (n=8, 3a (n=6, and 1a (n=4. By including reference sequences reported from elsewhere in China, phylogeographic trees were reconstructed to indicate their migration patterns. While the predominant 6a isolates were estimated to have origins in Guangdong and Guangxi provinces, the increase in 3b strains must have resulted from IDU network transmission from the southwest. A Bayesian Skyline Plot for subtype 1a, which is rare in China, showed a rapid population growth since 1998. Although slowed in rate around 2005, this growth continued to the present. Not found for any other HCV lineage. Conclusions: Overall, a delayed growth pattern may indicate the unique history of 1a dissemination in China and its recently increasing prevalence, despite measures taken to improve HCV prevention.

  17. HCMV gB genotypes in cervical secretion and placenta tissues in the state of Espírito Santo, Southeastearn Brazil Genótipos gB de citomegalovírus humano em secreção cervical e placenta no Espírito Santo, Sudeste do Brasil

    Directory of Open Access Journals (Sweden)

    Liliana Cruz Spano

    2007-09-01

    Full Text Available Human cytomegalovirus (HCMV displays genetic variability in several regions, supposed to be related with strain-specific tissue tropism and immunopathogenesis. Based on sequence variation in the UL55 gene that encodes gB glycoprotein, HCMV strains can be assigned to one of four genotypes. Previous studies have addressed gB genotyping mostly by investigating strains derived from immunosuppressed patients, sometimes without previous knowledge about genotype distribution in a geographic area. The present study verified the distribution of HCMV gB genotypes of strains obtained from immunocompetent women at Vitória City, Espírito Santo State, Southeastern, Brazil. The HCMV genome was extracted from their cervical secretion, fetal and maternal placenta tissues (chorionic villous and decidua from abortion cases and from white blood cells (WBCs. HCMV genotyping was performed by restriction fragment length polymorphism analyses of amplified product from the high variability site of the UL55 gene. All four genotypes were observed in both cervical secretion and placenta, whereas in WBCs a single gB1 genotype was detected. HCMV gB1 and gB2 genotypes were detected, respectively, in nine and in six of the 23 studied samples, while gB3 and gB4 were each found in four separate samples of the total. The differences in genotype frequency were not considered statistically significant. No mixed genotype infection was observed. The results indicated that the four gB HCMV genotypes had no particular tropism for placenta tissues and that all genotypes circulated within immunocompetent women at the time and in the region of study.O citomegalovírus humano (HCMV apresenta variabilidade em diversas regiões do genoma, supostamente relacionada ao tropismo tecidual e imunopatogênese viral. Baseando-se na variação de seqüência do gene UL55 que codifica a glicoproteína gB, o HCMV pode ser classificado em um dos quatro genótipos. Estudos prévios têm investigado a

  18. EVALUATION OF CIRCUMSPOROZOITE PROTEIN OF Plasmodium vivax TO ESTIMATE ITS PREVALENCE IN OIAPOQUE , AMAPÁ STATE, BRAZIL, BORDERING FRENCH GUIANA

    Science.gov (United States)

    GOMES, Margarete do Socorro Mendonça; VIEIRA, José Luiz Fernandes; CASSIANO, Gustavo Capatti; MUSSET, Lise; LEGRAND, Eric; NACHER, Mathieu; COUTO, Vanja Suely Calvosa D'Almeida; MACHADO, Ricardo Luiz Dantas; COUTO, Álvaro Augusto Ribeiro D'Almeida

    2016-01-01

    SUMMARY Malaria is a major health problem for people who live on the border between Brazil and French Guiana. Here we discuss Plasmodium vivax distribution pattern in the town of Oiapoque, Amapá State using the circumsporozoite (CS) gene as a marker. Ninety-one peripheral blood samples from P. vivax patients have been studied. Of these, 64 individuals were from the municipality of Oiapoque (Amapá State, Brazil) and 27 patients from French Guiana (August to December 2011). DNA extraction was performed, and a fragment of the P. vivax CS gene was subsequently analyzed using PCR/RFLP. The VK210 genotype was the most common in both countries (48.36% in Brazil and 14.28% in French Guiana), followed by the P. vivax-like (1.10% in both Brazil and French Guiana) and VK247 (1.10% only in Brazil) in single infections. We were able to detect all three CS genotypes simultaneously in mixed infections. There were no statistically significant differences either regarding infection site or parasitaemia among individuals with different genotypes. These results suggest that the same genotypes circulating in French Guiana are found in the municipality of Oiapoque in Brazil. These findings suggest that there may be a dispersion of parasitic populations occurring between the two countries. Most likely, this distribution is associated with prolonged and/or more complex transmission patterns of these genotypes in Brazil, bordering French Guiana. PMID:27680177

  19. Genotype Distribution and Molecular Epidemiology of Hepatitis C Virus in Hubei, Central China.

    Directory of Open Access Journals (Sweden)

    Jing Peng

    Full Text Available Little is known about the molecular epidemiology of hepatitis C virus (HCV infection in Central China.A total of 570 patients from Hubei Province in central China were enrolled. These patients were tested positive for HCV antibody prior to blood transfusion. Among them, 177 were characterized by partial NS5B and/or Core-E1 sequences and classified into five subtypes: 1b, 83.0% (147/177; 2a, 13.0% (23/177; 3b, 2.3% (4/177; 6a, 1.1% (2/177; 3a, 0.6% (1/177. Analysis of genotype-associated risk factors revealed that paid blood donation and transfusion before 1997 were strongly associated with subtypes 1b and 2a, while some subtype 2a cases were also found in individuals with high risk sexual behaviors; subtypes 3b, 6a, and 3a were detected only in intravenous drug users. Phylogeographic analyses based on the coalescent datasets demonstrated that 1b, 2a, 3b, and 6a were locally epidemic in Hubei Province. Among them, subtype 1b Hubei strains may have served as the origins of this subtype in China, and 2a and 3b Hubei strains may have descended from the northwest and southwest of China, respectively, while 6a Hubei strains may have been imported from the central south and southwest.The results suggest that the migration patterns of HCV in Hubei are complex and variable among different subtypes. Implementation of mandatory HCV screening before donation has significantly decreased the incidence of transfusion-associated HCV infection since 1997. More attention should be paid to intravenous drug use and unsafe sexual contact, which may have become new risk factors for HCV infection in Hubei Province.

  20. Prevalence and Distribution of High-Risk Genotypes of HPV in Women with Severe Cervical Lesions in Madrid, Spain: Importance of Detecting Genotype 16 and Other High-Risk Genotypes.

    Science.gov (United States)

    Mateos Lindemann, Maria Luisa; Sánchez Calvo, Juan Manuel; Chacón de Antonio, Jesús; Sanz, Itziar; Diaz, Esperanza; Rubio, Maria Dolores; de la Morena, Maria Luisa

    2011-01-01

    Background. Persistent infection with high-risk human papillomavirus (HR-HPV) has been demonstrated to be the necessary causal factor for developing cervical cancer. To know the most prevalent HR-HPV in different geographical areas is important to design diagnostic tests and implementation of vaccines. Objectives. The goal of this study is to evaluate the prevalence of HR-HPV in a total of 1001 patients, 198 with normal cytology results, 498 with low-grade squamous intraepithelial lesion (LSIL), and 205 with high-grade squamous intraepithelial lesion (HSIL) who attended our gynaecology department for opportunistic screening of HPV infection. Study design. Cervical samples were taken in a PreservCyt vial (Cytyc Corporation, Boxborough, MA). Hybrid capture assay was carried out following the manufacturer's instructions (Digene Corp., Gaithersburg, MD). All samples were further studied with polymerase chain reaction (PCR) (Linear Array HPV Genotyping Test, Roche Diagnostics, Mannheim, Germany). Results. Genotype 16 was the most prevalent HR-HPV in the three groups, 17.8% in the patients with normal cytology results, 22.3% in the LSIL group, and 60% in the HSIL group. Genotype 18 had a very low prevalence in all groups. Other HR-HPV genotypes such as genotype 31, genotype 58 and genotype 52 were found in significant numbers in HSIL patients. Discussion. Our data show that genotypes 16, 31, 58, and 52 are the most prevalent HR-HPV in cervical samples with severe intraepithelial lesion in Spain. There may be some geographical variation in prevalence of carcinogenic types, and it must be considered for designing diagnostic tests and vaccine.

  1. Spatial distribution and socioeconomic context of tuberculosis in Rio de Janeiro, Brazil

    Directory of Open Access Journals (Sweden)

    Alessandra Gonçalves Lisbôa Pereira

    2015-01-01

    Full Text Available OBJECTIVE To analyze the spatial distribution of risk for tuberculosis and its socioeconomic determinants in the city of Rio de Janeiro, Brazil.METHODS An ecological study on the association between the mean incidence rate of tuberculosis from 2004 to 2006 and socioeconomic indicators of the Censo Demográfico (Demographic Census of 2000. The unit of analysis was the home district registered in the Sistema de Informação de Agravos de Notificação (Notifiable Diseases Information System of Rio de Janeiro, Southeastern Brazil. The rates were standardized by sex and age group, and smoothed by the empirical Bayes method. Spatial autocorrelation was evaluated by Moran’s I. Multiple linear regression models were studied and the appropriateness of incorporating the spatial component in modeling was evaluated.RESULTS We observed a higher risk of the disease in some neighborhoods of the port and north regions, as well as a high incidence in the slums of Rocinha and Vidigal, in the south region, and Cidade de Deus, in the west. The final model identified a positive association for the variables: percentage of permanent private households in which the head of the house earns three to five minimum wages; percentage of individual residents in the neighborhood; and percentage of people living in homes with more than two people per bedroom.CONCLUSIONS The spatial analysis identified areas of risk of tuberculosis incidence in the neighborhoods of the city of Rio de Janeiro and also found spatial dependence for the incidence of tuberculosis and some socioeconomic variables. However, the inclusion of the space component in the final model was not required during the modeling process.

  2. Geographic Distribution of Chagas Disease Vectors in Brazil Based on Ecological Niche Modeling

    Directory of Open Access Journals (Sweden)

    Rodrigo Gurgel-Gonçalves

    2012-01-01

    Full Text Available Although Brazil was declared free from Chagas disease transmission by the domestic vector Triatoma infestans, human acute cases are still being registered based on transmission by native triatomine species. For a better understanding of transmission risk, the geographic distribution of Brazilian triatomines was analyzed. Sixteen out of 62 Brazilian species that both occur in >20 municipalities and present synanthropic tendencies were modeled based on their ecological niches. Panstrongylus geniculatus and P. megistus showed broad ecological ranges, but most of the species sort out by the biome in which they are distributed: Rhodnius pictipes and R. robustus in the Amazon; R. neglectus, Triatoma sordida, and T. costalimai in the Cerrado; R. nasutus, P. lutzi, T. brasiliensis, T. pseudomaculata, T. melanocephala, and T. petrocchiae in the Caatinga; T. rubrovaria in the southern pampas; T. tibiamaculata and T. vitticeps in the Atlantic Forest. Although most occurrences were recorded in open areas (Cerrado and Caatinga, our results show that all environmental conditions in the country are favorable to one or more of the species analyzed, such that almost nowhere is Chagas transmission risk negligible.

  3. Comparison of spatial interpolation methods for the estimation of precipitation distribution in Distrito Federal, Brazil

    Science.gov (United States)

    Borges, Pablo de Amorim; Franke, Johannes; da Anunciação, Yumiko Marina Tanaka; Weiss, Holger; Bernhofer, Christian

    2016-01-01

    Available climatological information of Distrito Federal does not satisfy the requirements for detailed climate diagnosis, as they do not provide the necessary spatial resolution for water resources management purposes. Annual and seasonal climatology (1971-2000) of precipitation from 6 meteorological stations and 54 rain gauges from Central Brazil were used to test eight different spatial interpolation methods. Geographical factors (i.e., altitude, longitude and latitude) explain a large portion of precipitation in the region, and therefore, multivariate models were included. The performance of estimations was assessed through independent validation using mean square error, correlation coefficient and Nash-Sutcliffe efficiency criterion. Inverse distance weighting (IDW), ordinary kriging (OK) and the multivariate regression with interpolation of residuals by IDW (MRegIDW) and OK (MRegOK) have performed the lowest errors and the highest correlation and Nash-Sutcliffe efficiency criterion. In general, interpolation methods provide similar spatial distributions of rainfall wherever observation network is dense. However, the inclusion of geographical variables to the interpolation method should improve estimates in areas where the observation network density is low. Nevertheless, the assessment of uncertainties using a geostatistical method provides supplementary and qualitative information which should be considered when interpreting the spatial distribution of rainfall.

  4. The effects of non-agricultural activities on income distribution in rural Brazil

    Directory of Open Access Journals (Sweden)

    Henrique D. Neder

    2003-06-01

    Full Text Available The growth of non-agricultural activity since the 1980s has reconfigured the economic and social dynamic in Brazil’s rural areas. Our paper intends to describe the impact of this growth on income distribution in rural Brazil. We made use of a method of decomposition of two indicators of income distribution applied to1992 and 1999 Brazilian National Household Sample Survey (PNAD data: the variation coefficient and the Gini index. It was observed that non-agricultural activities have increased the concentration of rural income and that this phenomenon is more prominent in Region I (states of Brazil’s Northeast and the state of Tocantins and Region IV (states of São Paulo and Mato Grosso do Sul and the Distrito Federal. It was found that the income concentrating effect of each non-agricultural activity branch (commerce, transformation industry, administration, service differed by region. In Region I, income from the "Social/Public Administration and Other Activities branch " (a PNAD designation presents a coefficient of relative concentration greater than a unit, which indicates that income from this activities branch acts to increase the concentration of non-agricultural income in this region. In Region IV, income from industrial activities and from Social/ Public Administration and Other Activities both present coefficients of relative concentration greater than a unit; therefore, both branches act to increase the concentration of income from non-agricultural activities in this region.

  5. Serum uric acid distribution according to SLC22A12 W258X genotype in a cross-sectional study of a general Japanese population

    Directory of Open Access Journals (Sweden)

    Okada Rieko

    2011-03-01

    Full Text Available Abstract Background Although SLC22A12 258X allele was found among those with hypouricemia, it was unknown that serum uric acid distribution among those with SLC22A12 258X allele. This study examined serum uric acid (SUA distribution according to SLC22A12 W258X genotype in a general Japanese population. Methods Subjects were 5,023 health checkup examinees (3,413 males and 1,610 females aged 35 to 69 years with creatinine SLC22A12 W258X was genotyped with a polymerase chain reaction with confronting two-pair primers. Results The genotype frequency was 4,793 for WW, 225 for WX, and 5 for XX, which was in Hardy-Weinberg equilibrium (p = 0.164 with X allele 0.023 (95% confidence interval [0.021-0.027]. Mean (range SUA was 6.2 (2.1-11.4 mg/dL for WW, 3.9 (0.8-7.8 mg/dL for WX, and 0.8 (0.7-0.9 mg/dL for XX among males, and 4.5 (1.9-8.9 mg/dL, 3.3 (2.0-6.5 mg/dL, and 0.60 (0.5-0.7 mg/dL among females, respectively. Six individuals with SUA less than 1.0 mg/dL included two males with XX genotype, one male with WX genotype, and three females with XX genotype. Subjects with WX genotype were 14 (77.8% of 18 males with a SUA of 1.0-2.9 mg/dL, and 28 (34.6% of 81 females with the same range of SUA. The corresponding values were 131 (25.1% of 522 males and 37 (3.5% of 1,073 females for SUA 3.0-4.9 mg/dL, and 8 (0.4% of 2,069 males and 5 (1.1% of 429 females for SUA 5.0-6.9 mg/dL. The X allele effect for SUA less than 3 mg/dL was significantly (p Conclusions Although SLC22A12 W258X was a determining genetic factor on SUA, SUA of those with WX genotype distributed widely from 0.8 mg/dL to 7.8 mg/dL. It indicated that other genetic traits and/or lifestyle affected SUA of those with WX genotype, as well as those with WW genotype.

  6. Avaliação de genótipos de mandioca em diferentes épocas de colheita no Estado do Acre Evaluation of cassava genotypes in different harvesting times in the State of Acre, Brazil

    Directory of Open Access Journals (Sweden)

    Hélia Alves de Mendonça

    2003-06-01

    Full Text Available No Estado do Acre, a mandioca tem grande importância econômica e social, constituindo-se num dos principais produtos básicos da alimentação da população, principalmente na forma de farinha, mas com grande potencial também para o consumo in natura. O objetivo deste trabalho foi avaliar genótipos de mandioca em diferentes épocas de colheita no Estado do Acre. Foram avaliados dez genótipos de mandioca em quatro épocas de colheita utilizando o delineamento de blocos casualizados com quatro repetições, em esquema de parcelas subdivididas, sendo as épocas as parcelas e os genótipos as subparcelas, nas safras 1999/2000 e 2000/2001. Os genótipos MD-33 e Pão apresentaram alto rendimento de raízes e resistência à podridão radicular; o primeiro é indicado para a industrialização e o segundo para o consumo in natura. A colheita aos 14 meses após o plantio proporcionou o maior rendimento de raízes, mas apresentou maior incidência de podridão radicular. O teor de amido e a incidência de podridão radicular variaram em razão dos genótipos e épocas de colheita avaliados.The cassava has a great economical and social importance as a basic food for the population of the State of Acre, Brazil. It is used to produce flour and it has a great potential for fresh consumption too. The aim of this work was to evaluate genotypes of cassava at different harvesting times in the State of Acre, Brazil. Ten cassava genotypes were evaluated at four harvesting times in a randomized block experimental design, arranged in a split-plot scheme with four replications, with the harvesting time in the plots and the genotypes in the subplots, during the 1999/2000 and 2000/2001 seasons. The MD-33 and Pão genotypes presented high root yield and resistance to the root rot. The MD-33 genotype is indicated for industrialization and the Pão genotype for fresh consumption. Harvesting 14 months after planting resulted in the highest root yield, but presented

  7. Genetic parameters and investigation of genotype × environment interactions in Nellore × Hereford crossbred for resistance to cattle ticks in different regions of Brazil.

    Science.gov (United States)

    Ayres, D R; Pereira, R J; Boligon, A A; Baldi, F; Roso, V M; Albuquerque, L G

    2015-02-01

    Data from 6,156 Nellore × Hereford crossbred cattle, distributed in 18 herds located in the Brazilian states of Mato Grosso do Sul (MS), São Paulo (SP), Paraná (PR) and Rio Grande do Sul (RS), were analysed in order to investigate genetic variation for resistance to the cattle tick through the estimation of variance components and genetic parameters for counting ticks (Rhipicephalus (Boophilus) microplus) in natural infestation, and also a possible genotype × environment interaction. The tick count data (CC) were transformed to log10(CC + 1) and grouped into two regions, defined by cluster analysis and analysed using the method of restricted maximum likelihood. The statistical model included the additive genetic effect as random and fixed effects of the contemporary group (CG) and genetic group (GG) as classificatory and the age of the animal at the time of counting (linear effect) and individual heterozygosity (linear effect, ranging from 0 to 1) as covariates. In the studied regions, the effect of heterosis found was inversely proportional; in other words, the count of ticks decreased with the increase of heterozygosity. The observed heritability estimation for resistance to tick infestation were 0.12 ± 0.04 and 0.11 ± 0.04 for groups A (RS and south PR) and B (MS, SP and north PR), respectively. The results of this study suggest that selection for animals resistant to ticks would be possible using the tick count to estimate the genetic value of animals, but with a slow genetic progress. The genetic correlation for tick count between the two groups (A and B) was 0.84 ± 0.27 and genotype × environment interaction for this trait was not observed in the studied population. PMID:25108748

  8. Serotype and genotype distribution among invasive Streptococcus pneumoniae isolates in Colombia, 2005-2010.

    Science.gov (United States)

    Parra, Eliana L; Ramos, Viviana; Sanabria, Olga; Moreno, Jaime

    2014-01-01

    In Colombia, a laboratory-based surveillance of invasive Streptococcus pneumoniae isolates as part of SIREVA II PAHO has been conducted since 1994. This study describes the serotype distribution, antimicrobial resistance, and genetic relationships of pneumococcal isolates recovered in Colombia from 2005 to 2010. In this study, demographic data of invasive S. pneumoniae isolates were analyzed, and antimicrobial susceptibility patterns were determined. Pulse field gel electrophoresis (n = 629) and multilocus sequence typing (n = 10) were used to determine genetic relationship of isolates with minimal inhibitory concentration to penicillin ≥0.125 µg/mL. A total of 1775 isolates of S. pneumoniae were obtained. Fifteen serotypes accounted for 80.7% of isolates. Serotype 14 (23.1%) was the most frequent in the general population. Penicillin resistance was 30.7% in meningitis and 9.0% in non-meningitis. Clones Spain(6B)ST90, Spain(9V)ST156, Spain(23F)ST81, and Colombia(23F)ST338 were associated to isolates. Additionally, serotype 6A isolates were associated with ST460 and ST473, and 19A isolates with ST276, ST320, and ST1118. In conclusion, the surveillance program provided updated information of trends in serotype distribution, antimicrobial resistance and the circulation of clones in invasive pneumococcal diseases. These results could be helpful to understand the epidemiology of S. pneumoniae in Colombia, and provide a baseline to measure the impact of vaccine introduction.

  9. The Impact of Global Climate Change on the Geographic Distribution and Sustainable Harvest of Hancornia speciosa Gomes (Apocynaceae) in Brazil

    Science.gov (United States)

    Nabout, João Carlos; Magalhães, Mara Rúbia; de Amorim Gomes, Marcos Aurélio; da Cunha, Hélida Ferreira

    2016-04-01

    The global Climate change may affect biodiversity and the functioning of ecosystems by changing the appropriate locations for the development and establishment of the species. The Hancornia speciosa, popularly called Mangaba, is a plant species that has potential commercial value and contributes to rural economic activities in Brazil. The aim of this study was to evaluate the impact of global climate change on the potential geographic distribution, productivity, and value of production of H. speciosa in Brazil. We used MaxEnt to estimate the potential geographic distribution of the species in current and future (2050) climate scenarios. We obtained the productivity and value of production for 74 municipalities in Brazil. Moreover, to explain the variation the productivity and value of production, we constructed 15 models based on four variables: two ecological (ecological niche model and the presence of Unity of conservation) and two socio-economic (gross domestic product and human developed index). The models were selected using Akaike Information Criteria. Our results suggest that municipalities currently harvesting H. speciosa will have lower harvest rates in the future (mainly in northeastern Brazil). The best model to explain the productivity was ecological niche model; thus, municipalities with higher productivity are inserted in regions with higher environmental suitability (indicated by niche model). Thus, in the future, the municipalities harvesting H. speciosa will produce less because there will be less suitable habitat for H. speciosa, which in turn will affect the H. speciosa harvest and the local economy.

  10. The Impact of Global Climate Change on the Geographic Distribution and Sustainable Harvest of Hancornia speciosa Gomes (Apocynaceae) in Brazil.

    Science.gov (United States)

    Nabout, João Carlos; Magalhães, Mara Rúbia; de Amorim Gomes, Marcos Aurélio; da Cunha, Hélida Ferreira

    2016-04-01

    The global Climate change may affect biodiversity and the functioning of ecosystems by changing the appropriate locations for the development and establishment of the species. The Hancornia speciosa, popularly called Mangaba, is a plant species that has potential commercial value and contributes to rural economic activities in Brazil. The aim of this study was to evaluate the impact of global climate change on the potential geographic distribution, productivity, and value of production of H. speciosa in Brazil. We used MaxEnt to estimate the potential geographic distribution of the species in current and future (2050) climate scenarios. We obtained the productivity and value of production for 74 municipalities in Brazil. Moreover, to explain the variation the productivity and value of production, we constructed 15 models based on four variables: two ecological (ecological niche model and the presence of Unity of conservation) and two socio-economic (gross domestic product and human developed index). The models were selected using Akaike Information Criteria. Our results suggest that municipalities currently harvesting H. speciosa will have lower harvest rates in the future (mainly in northeastern Brazil). The best model to explain the productivity was ecological niche model; thus, municipalities with higher productivity are inserted in regions with higher environmental suitability (indicated by niche model). Thus, in the future, the municipalities harvesting H. speciosa will produce less because there will be less suitable habitat for H. speciosa, which in turn will affect the H. speciosa harvest and the local economy.

  11. Molecular investigation of zoonotic genotypes of Giardia intestinalis isolates in humans, dogs and cats, sheep, goats and cattle in Araçatuba (São Paulo State, Brazil by the analysis of ß-giardin gene fragments

    Directory of Open Access Journals (Sweden)

    Elenir Alves Macedo de Godoy

    2013-10-01

    Full Text Available In the period from July 2009 to October 2010, fecal samples from 61 animals and 154 humans from the municipality of Aracatuba (São Paulo State, Brazil were studied. Fecal samples from animals were collected in the Municipal Animal Shelter and the Veterinary Hospital of the Universidade Estadual Paulista. Human fecal specimens were collected in playschools in the outskirts of the city by the private network of clinical analysis laboratories of the municipal. Diagnosis was done by optical microscopy using the Faust and Hoffmann, Pons and Janer techniques. The genotypes of Giardia intestinalis were characterized by PCR-RFLP and confirmed by sequencing the ß-giardin gene. Human specimens were positive in 25.3% (39/154 of the cases with 26.8% (36/134 of the specimens from children and 15% (3/20 from adults being positive. The frequency of G. intestinalis among the animals was 23.0% (14/61. A total of 32 isolates of G. intestinalis obtained from human feces and six from dogs and cats were characteristic of the A genotype (AI and AII/AIII. The results of this study in respect to frequency of giardiasis are similar to reported in most studies in Brazil. The prevalence observed in animal populations conforms to worldwide infection rates. G. intestinalis genotypes considered zoonotic were detected in both pets and humans from the city of Aractuba, suggesting a possible zoonotic transmission of the parasite in the northwestern region of São Paulo State. The absence of these genotypes in farm animals may imply that they are not involved in the chain of transmission to humans in this region.

  12. Diversity, natural history, and geographic distribution of snakes in the Caatinga, Northeastern Brazil.

    Science.gov (United States)

    Guedes, Thaís B; Nogueira, Cristiano; Marques, Otavio A V

    2014-09-19

    The present study is a synthesis on snake diversity and distribution in the Caatinga region of northeastern Brazil, providing an updated species list and data on natural history and geographic distribution. Our study is based on the careful revision of 7,102 voucher specimens, housed in 17 herpetological collections, complemented by data on taxonomic literature. We recorded a total of 112 snake species in the Caatinga, belonging to nine families: Anomalepididae, Leptotyphlopidae, Typhlopidae, Aniliidae, Boidae, Viperidae, Elapidae, Colubridae, and Dipsadidae. Our list includes at least 13 never recorded species for this region, as well as distribution records for all species known from the Caatinga (including expansion and new records of distribution). The snake assemblage of the Caatinga is complex, sharing species with other continental open areas (38.4%), forested areas (27.7%), and both open and forested areas (32.1%). The richest areas were isolated plateaus, followed by contact areas, semi-arid caatinga, and sandy dunes of the São Franscisco River. We identified 22 Caatinga endemic species with the sandy dunes of São Franscico River showing the highest endemism level (12 species, with six endemic species restricted to the area) followed by semi-arid caatinga, and isolated plateaus (eight endemic species each, and six and three endemic species with restricted distribution to each area, respectively). Most species show relatively restricted ranges in parts of the Caatinga. The snake assemblage in Caatinga includes mainly terrestrial species (38.4%), followed by fossorial/cryptozoic (26.8%), arboreal/semi-arboreal (26.8%), and aquatic/semi-aquatic (7.1%) species. Vertebrates are the most important dietary item (80.4%), with 56.6% of species being generalist consumers of this kind of prey; 24.4% are frog-eaters, 7.8% prey on caecilians/amphisbaenians, 6.7% lizard-eaters, 3.3% mammal-eaters, and 1.1% are fish-eaters. Only 18.7% of the snakes eat invertebrate

  13. Phlebotominae distribution in Janaúba, an area of transmission for visceral leishmaniasis in Brazil

    Directory of Open Access Journals (Sweden)

    Érika Monteiro Michalsky

    2009-02-01

    Full Text Available In Brazil, visceral leishmaniasis (VL is caused by Leishmania chagasi parasites that are transmitted to man through the bites of infected females of Lutzomyia longipalpis sand flies. In order to evaluate transmission risk and to clarify the epidemiology of this tropical disease, studies focused on the vector and favorable environmental conditions are of fundamental importance. In this work, we surveyed the phlebotomine sand fly fauna in Janaúba, a Brazilian municipality that is endemic for VL. During a two-year period, entomological captures were performed monthly in 15 districts with high, moderate and low profiles of VL transmission. A total of 14,591 phlebotomine sand flies were captured (92% L. longipalpis, with a predominance of males. Most specimens were captured in the peri-domicile setting, although the number of specimens captured in the intra-domicile setting emphasises the anthropophilic behaviour of this insect. The population density of L. longipalpis was modulated by climate variations, particularly with clear increases immediately after the rainy season. However, the pattern of distribution did not coincide with the occurrence of human or canine cases of VL. This suggests that the eco-epidemiology of VL is particular to each area of transmission and must be taken into account during the design of public health control actions.

  14. Phlebotominae distribution in Janaúba, an area of transmission for visceral leishmaniasis in Brazil.

    Science.gov (United States)

    Michalsky, Erika Monteiro; França-Silva, João Carlos; Barata, Ricardo Andrade; Lara e Silva, Fabiana de Oliveira; Loureiro, Angélica Marciano Fernandes; Fortes-Dias, Consuelo Latorre; Dias, Edelberto Santos

    2009-02-01

    In Brazil, visceral leishmaniasis (VL) is caused by Leishmania chagasi parasites that are transmitted to man through the bites of infected females of Lutzomyia longipalpis sand flies. In order to evaluate transmission risk and to clarify the epidemiology of this tropical disease, studies focused on the vector and favorable environmental conditions are of fundamental importance. In this work, we surveyed the phlebotomine sand fly fauna in Janaúba, a Brazilian municipality that is endemic for VL. During a two-year period, entomological captures were performed monthly in 15 districts with high, moderate and low profiles of VL transmission. A total of 14,591 phlebotomine sand flies were captured (92% L. longipalpis), with a predominance of males. Most specimens were captured in the peri-domicile setting, although the number of specimens captured in the intra-domicile setting emphasises the anthropophilic behaviour of this insect. The population density of L. longipalpis was modulated by climate variations, particularly with clear increases immediately after the rainy season. However, the pattern of distribution did not coincide with the occurrence of human or canine cases of VL. This suggests that the eco-epidemiology of VL is particular to each area of transmission and must be taken into account during the design of public health control actions.

  15. Distribution of radionuclides in the sediments of Cananeia-Iguape Estuarine Complex (SP, Brazil)

    Energy Technology Data Exchange (ETDEWEB)

    Ribeiro, Andreza P.; Ferreira, Paulo A.L.; Mahiques, Michel M., E-mail: andrezpr@usp.br, E-mail: paulo.alves.ferreira@usp.br, E-mail: mahiques@usp.br, E-mail: rfigueira@usp.br [Universidade de Sao Paulo (IO/USP), Sao Paulo, SP (Brazil). Instituto Oceanografico; Franca, Elvis J.; Figueira, Rubens C.L., E-mail: ejfranca@cnen.gov.br [Centro Regional de Ciencias Nucleares do Nordeste (CRCN-NE/CNEN-PE), Recife, PE (Brazil)

    2013-07-01

    With the advent of techniques that enabled the detection of radioactivity, a new frontier was opened in the many areas of Earth sciences, as the radionuclides can be used as tracers for processes of physical, chemical and biological natures. In this context, natural ({sup 40}K, {sup 232}Th and {sup 238}U) and artificial ({sup 137}Cs) radionuclides were measured through the means of high-resolution gamma spectrometry, a non-destructive technique, in the Cananeia-Iguape Estuarine Complex (Sao Paulo, Brazil). The activities obtained are 107.61 - 573.84 Bq kg{sup -1} for {sup 40}K, 11.11 - 73.65 Bq kg{sup -1} for {sup 232}Th, 2.27 - 60.76 Bq kg{sup -1} for {sup 238}U, and 0.23 - 3.49 Bq kg{sup -1} for {sup 137}Cs, with {sup 137}Cs content within the observed range for samples environmentally affected only by the fallout of past nuclear tests. Also, these radionuclides presented a significant (α = 0.05) correlation with grain size distribution and organic carbon content as well. (author)

  16. Distribution of radionuclides in the sediments of Cananeia-Iguape Estuarine Complex (SP, Brazil)

    International Nuclear Information System (INIS)

    With the advent of techniques that enabled the detection of radioactivity, a new frontier was opened in the many areas of Earth sciences, as the radionuclides can be used as tracers for processes of physical, chemical and biological natures. In this context, natural (40K, 232Th and 238U) and artificial (137Cs) radionuclides were measured through the means of high-resolution gamma spectrometry, a non-destructive technique, in the Cananeia-Iguape Estuarine Complex (Sao Paulo, Brazil). The activities obtained are 107.61 - 573.84 Bq kg-1 for 40K, 11.11 - 73.65 Bq kg-1 for 232Th, 2.27 - 60.76 Bq kg-1 for 238U, and 0.23 - 3.49 Bq kg-1 for 137Cs, with 137Cs content within the observed range for samples environmentally affected only by the fallout of past nuclear tests. Also, these radionuclides presented a significant (α = 0.05) correlation with grain size distribution and organic carbon content as well. (author)

  17. Spatial distribution of trace elements in topsoils adjacent to main avenues of Sao Paulo city, Brazil

    International Nuclear Information System (INIS)

    In the present study, the concentration and distribution of Ba, Cu, Mo, Pb, S, Zn and Zr in soils collected along two main avenues (Pinheiros River Highway and Tiete River Highway) with high traffic density in the metropolitan region of Sao Paulo, Brazil, are presented, and their possible sources are discussed. These elements are strongly considered as contaminants originated from vehicular emissions. The analytical technique employed was XRF. The data set was evaluated by a t test for independent samples (group: avenues) at a 0.05 significance level. According to t test, the average contents obtained from Pinheiros River Highways are significantly different than the Tiete River, except for Mo. Multivariate statistic approaches (Pearson Correlation, Cluster and Factorial Analysis - FA) were adopted for data treatment. FA identified two main factors which accounted for about 86% of the total variance. The behavior of Ba, Cu, Pb, S and Zn were explained by the Factor 1. This indicates that the elements may have similar sources, probably related to gas emissions escaping from the vehicle fuel system. Factor 2 included Mo and Zr, suggesting their origin in the sample soils may be associated with the deterioration process of some device in the vehicular engine system or may be associated with the chemical composition of the urban soil analyzed. (author)

  18. Spatial distribution of metals in soil samples from Zona da Mata, Pernambuco, Brazil using XRF technique

    International Nuclear Information System (INIS)

    Soil contamination is today one of the most important environmental issues for society. In the past, soil pollution was not considered as important as air and water contamination, because this was more difficult to be controlled, becoming an important topic in studies of environmental protection worldwide. Based on this, this paper provides information on the determination of metals in soil samples collected in Zona da Mata, Pernambuco, Brazil, where normally the application of pesticides, insecticides and other agricultural additives are used in a disorderly manner and without control. A total of 24 sampling points were monitored. The analysis of Mn, Fe, Ni, Zn, Br, Rb, Sr, Pb, Ti, La, Al, Si and P were performed using Energy Dispersive X-Ray Fluorescence. In order to assess the development of analytical method, inorganic Certified Reference Materials (IAEA-SOIL-7 and SRM 2709) were analyzed. In each sampling site, the geoaccumulation index were calculated to estimate the level of metal contamination in the soil, this was made taking into account the resolution 460 of the National Environmental Council (CONAMA in Portuguese). The elemental distribution patterns obtained for each metal were associated with different pollution sources. This assessment provides an initial description of pollution levels presented by metals in soils from several areas of Zona da Mata, providing quantitative evidence and demonstrating the need to improve the regulation of agricultural and industrial activities. (author)

  19. Distribution of dengue vectors in neighborhoods with different urbanization types of Manaus, state of Amazonas, Brazil

    Directory of Open Access Journals (Sweden)

    Claudia M Ríos-Velásquez

    2007-08-01

    Full Text Available Aedes aegypti and Ae. albopictus are vectors of dengue viruses, which cause endemic disease in the city of Manaus, capital of the state of Amazonas, Brazil. More than 53 thousand cases have been registered in this city since the first epidemic in 1998. We evaluated the hypothesis that different ecological conditions result in different patterns of vector infestation in Manaus, by measuring the infestation level in four neighborhoods with different urbanization patterns, during the rainy (April, dry (August, and transitional (November seasons. Ae. aegypti predominated throughout the study areas and sampling periods, representing 86% of all specimens collected in oviposition traps. High frequencies of houses positive for both species were observed in all studied sites, with Ae. aegypti present in more than 84% of the houses in all seasons. Ae. albopictus, on the other hand, showed more spatial and temporal variation in abundance. We found no association between infestation level and house traits. This study highlights the homogeneity of dengue vector distribution in Manaus.

  20. Spatial distribution of metals in soil samples from Zona da Mata, Pernambuco, Brazil using XRF technique

    Energy Technology Data Exchange (ETDEWEB)

    Fernandez, Zahily Herrero; Santos Junior, Jose Araujo dos; Amaral, Romilton dos Santos; Menezes, Romulo Simoes Cezar; Santos, Josineide Marques do Nascimento; Bezerra, Jairo Dias; Damascena, Kennedy Francys Rodrigues, E-mail: zahily1985@gmail.com, E-mail: jaraujo@ufpe.br, E-mail: romilton@ufpe.br, E-mail: rmenezes@ufpe.br, E-mail: neideden@hotmail.com, E-mail: jairo.dias@ufpe.br, E-mail: kennedy.eng.ambiental@gmail.com [Universidade Federal de Pernambuco (UFPE), Recife, PE (Brazil). Centro de Tecnologia e Geociencias. Departamento de Energia Nuclear; Alvarez, Juan Reinaldo Estevez, E-mail: jestevez@ceaden.cu [Centro de Aplicaciones Tecnologicas y Desarrollo Nuclear (CEADEN), Havana (Cuba); Silva, Edvane Borges da, E-mail: edvane.borges@pq.cnpq.br [Universidade Federal de Pernambuco (UFPE), Vitoria de Santo Antao, PE (Brazil). Nucleo de Biologia; Franca, Elvis Joacir de; Farias, Emerson Emiliano Gualberto de, E-mail: ejfranca@cnen.gov.br, E-mail: emersonemiliano@yahoo.com.br [Centro Regional de Ciencias Nucleares do Nordeste (CRCN-NE/CNEN-PE), Recife, PE (Brazil); Silva, Alberto Antonio da, E-mail: alberto.silva@barreiros.ifpe.edu.br [Instituto Federal de Educacao, Ciencia e Tecnologia de Pernambuco (IFPE), Barreiros, PE (Brazil)

    2015-07-01

    Soil contamination is today one of the most important environmental issues for society. In the past, soil pollution was not considered as important as air and water contamination, because this was more difficult to be controlled, becoming an important topic in studies of environmental protection worldwide. Based on this, this paper provides information on the determination of metals in soil samples collected in Zona da Mata, Pernambuco, Brazil, where normally the application of pesticides, insecticides and other agricultural additives are used in a disorderly manner and without control. A total of 24 sampling points were monitored. The analysis of Mn, Fe, Ni, Zn, Br, Rb, Sr, Pb, Ti, La, Al, Si and P were performed using Energy Dispersive X-Ray Fluorescence. In order to assess the development of analytical method, inorganic Certified Reference Materials (IAEA-SOIL-7 and SRM 2709) were analyzed. In each sampling site, the geoaccumulation index were calculated to estimate the level of metal contamination in the soil, this was made taking into account the resolution 460 of the National Environmental Council (CONAMA in Portuguese). The elemental distribution patterns obtained for each metal were associated with different pollution sources. This assessment provides an initial description of pollution levels presented by metals in soils from several areas of Zona da Mata, providing quantitative evidence and demonstrating the need to improve the regulation of agricultural and industrial activities. (author)

  1. Seasonal Variation and Frequency Distribution of Ectoparasites in Crossbreed Cattle in Southeastern Brazil

    Directory of Open Access Journals (Sweden)

    Maria do Socorro Ferraz da Costa

    2014-01-01

    Full Text Available The aims of this study were to evaluate the seasonal variation and frequency distribution of Rhipicephalus (Boophilus microplus, Haematobia irritans, and Dermatobia hominis on crossbred heifers under field conditions in the northeast of Minas Gerais state, southeastern Brazil. From November 2007 to September 2009 (23 months, 40 heifers aged 16.6±2.4 months were divided into groups A (1/4 Holstein × 3/4 Gir and B (1/2 Holstein × 1/2 Gir and had the monthly infestation estimated along with the climatic conditions. The mean maximum and minimum temperatures were 28.5 and 19°C, respectively. The ectoparasites were present on animals in all months of the year. The levels of ticks on the animals were low (3.0±0.2 ticks/animal, with the highest density in midwinter. The temperature was the climatic factor that most influenced the tick levels. The population of H. irritans (13.9±0.3 flies/animal and D. hominis (1.5±0.2 larvae/animal on heifers was more influenced by rainfall and exhibited two population peaks during the year. 1/2 Holstein heifers harbored significantly more H. irritans and D. hominis than 1/4 Holstein heifers. The results are discussed considering the most appropriate periods to apply ectoparasiticides and the genetic make-up of the animals.

  2. Altitudinal distribution of birds in a mountainous region in southeastern Brazil

    Directory of Open Access Journals (Sweden)

    Francisco Mallet-Rodrigues

    2010-08-01

    Full Text Available We studied the altitudinal distribution of 426 bird species in the Serra dos Órgãos, a mountainous region in southeastern Brazil. Thirty-four localities were visited between 1991 and 2009. Our study revealed a decline in bird species richness with elevation, although a smaller number of species was recorded at lower altitudes (below 300 m possibly due to local extinctions caused by the intense human occupation of the region. A less diverse avifauna was found above 2,000 m, with only one species (Caprimulgus longirostris recorded exclusively in this altitudinal range. Most endemic species were found between 300 and 1,200 m, but the endemism was more significant at higher altitudes. Nearly half of the birds found above 1,400 m were endemic species. Most of the threatened species from the state of Rio de Janeiro recorded in our study were found below 1,200 m, but no significant difference was found between the proportions of threatened species among different altitudinal ranges. Species of seventeen genera have exhibited some replacement (sometimes with partial overlap along altitudinal gradients.

  3. Skeletonema potamos (Bacillariophyta in Patos Lagoon, southern Brazil: Taxonomy and distribution

    Directory of Open Access Journals (Sweden)

    Lezilda Carvalho Torgan

    2011-07-01

    Full Text Available We analyzed the morphogical features of the centric diatom Skeletonema potamos (Weber Hasle from Patos Lagoon, southern Brazil, using light and scanning electron microscopy. We discuss the abundance and dis- tribution of the species along the salinity gradient in the lagoon. Samples from the water surface were taken monthly at eight stations along the longitudinal axis of the lagoon, from December 1987 to December 1988. The species were counted by the Utermöhl method, and the density (cells.mL-1 was estimated based on live cells. The morphology of the specimens agrees with the type, from the Little Miami River, Ohio, U.S.A., except for the convexity and the pattern of granules on the valve face. Skeletonema potamos was found in the winter and spring, and was distributed in the limnetic, oligohaline and mesohaline zones of the lagoon. The cell con- centration appeared to be controlled by the salinity, with a significant negative correlation observed. Light and competition probably also influence the development of S. potamos populations in the Patos Lagoon.

  4. Prevalence and geospatial distribution of bovine cysticercosis in the state of Mato Grosso, Brazil.

    Science.gov (United States)

    Rossi, Gabriel Augusto Marques; de Simoni, Heloisa Adélia Stefanoni; Lopes, Welber Daniel Zanetti; Almeida, Henrique Meiroz de Souza; Soares, Vando Edésio; Vidal, Ana Maria Centola; Ferraudo, Antonio Sergio; Mathias, Luis Antonio

    2016-08-01

    This study focused on estimating the prevalence and evaluating the geospatial distribution of bovine cysticercosis in the state of Mato Grosso, Brazil. To this, we used data of 6,200,497 animals slaughtered during the years of 2013 and 2014, and from 141 municipalities of the state. The prevalence observed for this period was 0.0873% (95% CI 0.0851-0.0897). Regarding the cysticerci detected, the calcified ones were the most frequent (74.43%). The high odds ratios were observed in animals reared in the Administrative Regions of Sinop, Barra do Garças, Água Boa, Cáceres, Barra do Bugres, Cuiabá, Pontes Lacerda, Rondonópolis, Matupa, São Félix do Araguaia and Lucas do Rio Verde, respectively. Furthermore, the results indicate the existence of a relation between the areas with high cysticercosis prevalence and human population density. We highlight the need of the development of a risk model based on the origin to improve cysticercosis detection in endemic areas. PMID:27435651

  5. Natural radionuclides distribution in the shelf and upper slope of southeast Brazil

    Energy Technology Data Exchange (ETDEWEB)

    Cordero, Luisa M.; Figueira, Rubens C.L., E-mail: luisa.cordero@usp.b, E-mail: rfigueira@usp.b [Universidade de Sao Paulo (USP), SP (Brazil). Inst. Oceanografico. Lab. de Quimica Inorganica Marinha; Mahiques, Michel M., E-mail: mahiques@usp.b [Universidade de Sao Paulo (USP), SP (Brazil). Inst. Oceanografico. Lab. de Analise de Materia Organica; Tessler, Moyses G., E-mail: mgtessle@usp.b [Universidade de Sao Paulo (USP), SP (Brazil). Inst. Oceanografico. Lab. de Espectrometria Gama

    2009-07-01

    In recent decades, Oceanography has been using a variety of radionuclides as tracers to understand the ocean dynamic processes, handling and disposal of sediments of seabed. In this context, the determination of natural radionuclides distributions ({sup 238}U, {sup 232}Th and {sup 40}K) has been carried out with sediments samples from the shelf and upper slope off Southeast Brazil using a gamma spectrometry technique. The samples were sliced into strata of 2 cm, dried, ground and properly packed to be analysed. The concentration of activities was performed in a hyperpure Ge detector with a resolution of 1,9 keV for the peak of 1332,3 keV of {sup 60}Co, model GEM50P by EGG and ORTEC. The study area is located between latitudes 28 deg 40'S and 23 deg 00'S and extends from Cabo Frio (RJ) to Cabo de Santa Marta Grande (SC). The activity concentrations varied from 0,6 to 52,8 BqKg{sup -1} for {sup 238}U, from 1,6 to 50,9BqKg{sup -1} for {sup 232}Th and from 65,4 to 873,3 BqKg{sup -1} for {sup 40}K. From these results it is possible to establish a correlation between the depositional area dynamics and the samples size parameters. (author)

  6. Fasciola hepatica IN BOVINES IN BRAZIL: DATA AVAILABILITY AND SPATIAL DISTRIBUTION

    Directory of Open Access Journals (Sweden)

    Sita C. Bennema

    2014-01-01

    Full Text Available Fasciolosis is a disease of importance for both veterinary and public health. For the first time, georeferenced prevalence data of Fasciola hepatica in bovines were collected and mapped for the Brazilian territory and data availability was discussed. Bovine fasciolosis in Brazil is monitored on a Federal, State and Municipal level, and to improve monitoring it is essential to combine the data collected on these three levels into one dataset. Data were collected for 1032 municipalities where livers were condemned by the Federal Inspection Service (MAPA/SIF because of the presence of F. hepatica. The information was distributed over 11 states: Espírito Santo, Goiás, Minas Gerais, Mato Grosso do Sul, Mato Grosso, Pará, Paraná, Rio de Janeiro, Rio Grande do Sul, Santa Catarina and São Paulo. The highest prevalence of fasciolosis was observed in the southern states, with disease clusters along the coast of Paraná and Santa Catarina and in Rio Grande do Sul. Also, temporal variation of the prevalence was observed. The observed prevalence and the kriged prevalence maps presented in this paper can assist both animal and human health workers in estimating the risk of infection in their state or municipality.

  7. Distribution, abundance and biomass of Chaetognaths off São Sebastião region, Brazil in February 1994

    OpenAIRE

    Tsui-Hua Liang; Luz Amelia Vega-Perez

    2002-01-01

    The distribution, abundance, biomass, population structure and feeding habits of chaetognaths collected off São Sebastião region, Brazil, in February 1994 are described. Bongo nets were hauled obliquely to collect zooplankton samples. Forty-three samples obtained with the 333 urn mesh were analysed. In this study, 7 chaetognath species belonging to two genera were identified. Sagitta friderici, S. tenuis and S. bipunctata were grouped into the neritic category, and Sagitta enflata, S. hispida...

  8. Diversity and distribution of the bryophyte flora in montane forests in the Chapada Diamantina region of Brazil

    OpenAIRE

    Emilia de Brito Valente; Kátia Cavalcanti Pôrto; Cid José Passos Bastos; Jana Ballejos-Loyola

    2013-01-01

    Bryophytes constitute an important component of tropical rain forests, which provide microhabitats favorable for their establishment. Bryophytes are also quite responsive to changes in microclimate, which makes them good bioindicators. This study aimed to determine the diversity and distribution of bryophytes in upper and lower montane forests of the Chapada Diamantina region of the state of Bahia, Brazil. To that end, we studied community aspects such as richness, diversity, substrates colon...

  9. Local distribution and abundance of Cardisoma guanhumi Latreille, 1928 (Brachyura: Gecarcinidae) in southern Brazil.

    Science.gov (United States)

    Oliveira-Neto, J F; Batista, E; Metri, R; Metri, C B

    2014-02-01

    The blue land crab, Cardisoma guanhumi Latreille, 1828 (Brachyura: Gecarcinidae) is officially included in the list of over-exploited species in Brazil, although still abundantly found in the state of Santa Catarina, the southern limit of its distribution. This species was found in forested areas, gardens, and grassy areas, including crabs with carapace width larger than 80mm. The existence of this population with these characteristics is surprising, since there is only one official record of the species in the southern region. The objectives of this study are to estimate the abundance and occupation patterns of C. guanhumi in this region. Correlations with conservation were discussed. The absolute abundance of crabs in the middle of summer activity was established for an area of 100,000 m2. A smaller area was mapped and divided into sampling units for statistical analyses. We distributed approximately 240 crabs in a forested area of about 3,000 m2 and 150 crabs in grassy areas (90,000 m2). The statistical test of Kruskal-Wallis test showed that there are significant differences between the sizes of the openings of the galleries inside the forest and that located in grassy areas. In the forest, the openings tend to be much larger. Burrows were found at a distance of 150 metres from the channel. The number of galleries was higher in the forested area, although the burrows were more densely grouped in grassy areas. Although C. guanhumi seems to be adjusting well to changes caused by human occupation, small forested areas are more conducive to growth and conservation of this species.

  10. Spatial distribution of mollusks in the intertidal zone of sheltered beaches in southeastern of Brazil

    Directory of Open Access Journals (Sweden)

    Eliane P. de Arruda

    2003-06-01

    Full Text Available The spatial distribution of mollusks in the intertidal zone was examined monthly from August 1995 through July 1997, in Enseada, Barra Velha and Araçá beaches in southeastern of Brazil. One study sector was selected in Enseada and Barra Velha, and two sectors in Araçá (Araçá I and Araçá II. The sectors were 10 m wide and equivalent in length to the width of the intertidal zone. Each sector was divided into three horizontal levels: lower, middle and upper, where the samples were taken with a cylinder corer with a base area of 0.16 m². In order to characterize the intertidal environment in these areas, some environmental variables were analyzed. In general, the mollusks were distributed in the sectors as follows: Enseada - Olivella minuta (Link, 1807 in the lower level and Tagelus plebeius (Lightfoot, 1786 in the upper level; Araçá I - O. minuta in the lower level, Tellina lineata Turton, 1819 and Anomalocardia brasiliana (Gmelin, 1791 in the middle levels; Araçá II - Cerithium atratum (Born, 1778 in the lower level, O. minuta in the lower and middle levels, and A. brasiliana and Corbula caribaea Orbigny, 1842 in the middle level; Barra Velha - Tagelus divisus (Spengler, 1794, Lucina pectinata (Gmelin, 1791 and Tellina versicolor De Kay, 1843 in the lower level, and A. brasiliana and Macoma constricta (Brugüìere, 1792 in the upper level. The intertidal zone of the study sectors could be divided into two biological zones: the upper zone, where T. plebeius, A. brasiliana and M. constricta were more abundant; and the lower zone, where O. minuta, C. atratum, T. lineata, T. versicolor, C. caribaea, T. divisus and L. pectinata were abundant.

  11. Spatial distribution of schistosomiasis and geohelminthiasis cases in the rural areas of Pernambuco, Brazil

    Directory of Open Access Journals (Sweden)

    Verônica Santos Barbosa

    2012-10-01

    Full Text Available INTRODUCTION: The prevalence and intensity of geohelminth infections and schistosomiasis remain high in the rural areas of Zona da Mata, Pernambuco (ZMP, Brazil, where these parasites still represent a significant public health problem. The present study aimed to spatially assess the occurrences of schistosomiasis and geohelminthiasis in the ZMP. METHODS: The ZMP has a population of 1,132,544 inhabitants, formed by 43 municipalities. An ecological study was conducted, using secondary data relating to positive human cases and parasite loads of schistosomiasis and positive human cases of geohelminthiasis that were worked up in Excel 2007. We used the coordinates of the municipal headquarters to represent the cities which served as the unit of analysis of this study. The Kernel estimator was used to spatially analyze the data and identify distribution patterns and case densities, with analysis done in ArcGIS software. RESULTS: Spatial analysis from the Kernel intensity estimator made it possible to construct density maps showing that the northern ZMP was the region with the greatest number of children infected with parasites and the populations most intensely infected by Schistosoma mansoni. In relation to geohelminths, there was higher spatial distribution of cases of Ascaris lumbricoides and Trichuris trichiura in the southern ZMP, and greater occurrence of hookworms in the northern/central ZMP. CONCLUSIONS: Despite several surveys and studies showing occurrences of schistosomiasis and geohelminthiasis in the ZMP, no preventive measures that are known to have been effective in decreasing these health hazards have yet been implemented in the endemic area.

  12. Water pollution and distribution of the black fly (Diptera: Simuliidae) in the Atlantic Forest, Brazil.

    Science.gov (United States)

    Docile, Tatiana N; Figueiró, Ronaldo; Gil-Azevedo, Leonardo H; Nessimian, Jorge L

    2015-09-01

    Black flies have medical importance because some species are vectors of the unenocerciasis and Mansonelosis, nevertheless, their ecology and potential use as bioindicators is still poorly studied in the Neotropical Region. In Brazil, bioindicators use is strongly focused in a multimetrical ecological index approach; this way, we investigated the black fly spatial distribution, in relation to abiotic factors correlated to water quality, to provide baseline information for their utilization as standalone indicators of lotic systems integrity. We have tested the hypothesis that environmental changes related to urbanization, lead to decreased abundance and loss in the number of species of the black fly fauna. The sampling was conducted in 10 urban and 10 preserved streams during the dry season (August-September) of 2012, in the mountainous region of Teres6polis, State of Rio de Janeiro, Brazil. The streams were characterized for their environmental integrity conditions and physico-chemical properties of water. In each stream, five different rapid points were sampled in a section of 50 meters, 10 meters apart from each other. The black flies were sampled with a kick-net sampler on rocky substrates. The material was separated and the larvae were sorted in morphotypes, and later, the final instar specimens were dissected and identified with the help of taxonomical literature at species level. A total abundance of 488 larvae from nine species were collected, 5 (1.02 %) in extremely impacted streams, 470 (96.31 %) in intermediate streams and 13 (2.66 %) in preserved streams. The visual evaluation (HII) differed in relation to the water physico-chemical evaluation, in which more variation in the characterization of the sampling sites was observed. In Canonical Correspondence Analysis Simulium subpallidum, S. inscrustatum and S. pertinax were significantly associated with intermediate values of most of the variables, and then to intermediate impacted sites. On the other hand

  13. Water pollution and distribution of the black fly (Diptera: Simuliidae) in the Atlantic Forest, Brazil.

    Science.gov (United States)

    Docile, Tatiana N; Figueiró, Ronaldo; Gil-Azevedo, Leonardo H; Nessimian, Jorge L

    2015-09-01

    Black flies have medical importance because some species are vectors of the unenocerciasis and Mansonelosis, nevertheless, their ecology and potential use as bioindicators is still poorly studied in the Neotropical Region. In Brazil, bioindicators use is strongly focused in a multimetrical ecological index approach; this way, we investigated the black fly spatial distribution, in relation to abiotic factors correlated to water quality, to provide baseline information for their utilization as standalone indicators of lotic systems integrity. We have tested the hypothesis that environmental changes related to urbanization, lead to decreased abundance and loss in the number of species of the black fly fauna. The sampling was conducted in 10 urban and 10 preserved streams during the dry season (August-September) of 2012, in the mountainous region of Teres6polis, State of Rio de Janeiro, Brazil. The streams were characterized for their environmental integrity conditions and physico-chemical properties of water. In each stream, five different rapid points were sampled in a section of 50 meters, 10 meters apart from each other. The black flies were sampled with a kick-net sampler on rocky substrates. The material was separated and the larvae were sorted in morphotypes, and later, the final instar specimens were dissected and identified with the help of taxonomical literature at species level. A total abundance of 488 larvae from nine species were collected, 5 (1.02 %) in extremely impacted streams, 470 (96.31 %) in intermediate streams and 13 (2.66 %) in preserved streams. The visual evaluation (HII) differed in relation to the water physico-chemical evaluation, in which more variation in the characterization of the sampling sites was observed. In Canonical Correspondence Analysis Simulium subpallidum, S. inscrustatum and S. pertinax were significantly associated with intermediate values of most of the variables, and then to intermediate impacted sites. On the other hand

  14. High-risk human papilloma virus genotypes in cervical carcinoma of Serbian women: Distribution and association with pathohistological findings.

    Science.gov (United States)

    Stamenković, Miodrag; Knežević, Aleksandra; Knežević, Ivana; Kuzmanović, Igor; Karalić, Danijela; Milenković, Sanja; Jovanović, Tanja

    2016-09-01

    A significant role of high-risk Human papilloma viruses (HR HPV) in the development of cervical carcinoma is well known. HR HPV 16 and 18 account for approximately 70% of all cases of cervical cancer worldwide. The incidence of cervical cancer in Serbia, is one of the highest in Europe. The aim of our study was to investigate the distribution of HR HPV types in cervical carcinoma of Serbian women, as well as association between the HPV types and pathohistological findings. The study included 80 archival cervical cancer tissues from the same number of patients. The presence of HPV DNA was determined using MY09/MY11 primers for L1 gene and GP1/GP2 primers for E1 gene. HPV was detected in 78.75% tissues. HR HPV genotypes found in the decreasing order of frequency were: HPV16 (80.39%), HPV33 (7.84%), HPV58 (5.88%), HPV18 (1.96%), HPV45 (1.96%) and HPV53 (1.96%). The examined tissues were 91.25% squamous cell carcinomas and 8.75% adenocarcinoma. The high frequency of HPV 16 was observed in both types of carcinoma (80.8% and 75%, respectively) while the prevalence of HPV18 was low. These results may contribute to the implementation of cervical carcinoma prevention program in Serbia, including the selection of the most appropriate vaccine and immunization program. PMID:27461126

  15. Spontaneous viral clearance, viral load, and genotype distribution of hepatitis C virus (HCV) in HIV-infected patients with anti-HCV antibodies in Europe

    DEFF Research Database (Denmark)

    Soriano, Vincent; Mocroft, Amanda; Rockstroh, Juergen;

    2008-01-01

    BACKGROUND: Variables influencing serum hepatitis C virus (HCV) RNA levels and genotype distribution in individuals with human immunodeficiency virus (HIV) infection are not well known, nor are factors determining spontaneous clearance after exposure to HCV in this population. METHODS: All HCV...... antibody (Ab)-positive patients with HIV infection in the EuroSIDA cohort who had stored samples were tested for serum HCV RNA, and HCV genotyping was done for subjects with viremia. Logistic regression was used to identify variables associated with spontaneous HCV clearance and HCV genotype 1. RESULTS......: Of 1940 HCV Ab-positive patients, 1496 (77%) were serum HCV RNA positive. Injection drug users (IDUs) were less likely to have spontaneously cleared HCV than were homosexual men (20% vs. 39%; adjusted odds ratio [aOR], 0.36 [95% confidence interval {CI}, 0.24-0.53]), whereas patients positive...

  16. Whole genome comparisons suggest random distribution of Mycobacterium ulcerans genotypes in a Buruli ulcer endemic region of Ghana.

    Directory of Open Access Journals (Sweden)

    Anthony S Ablordey

    2015-03-01

    Full Text Available Efforts to control the spread of Buruli ulcer--an emerging ulcerative skin infection caused by Mycobacterium ulcerans--have been hampered by our poor understanding of reservoirs and transmission. To help address this issue, we compared whole genomes from 18 clinical M. ulcerans isolates from a 30 km2 region within the Asante Akim North District, Ashanti region, Ghana, with 15 other M. ulcerans isolates from elsewhere in Ghana and the surrounding countries of Ivory Coast, Togo, Benin and Nigeria. Contrary to our expectations of finding minor DNA sequence variations among isolates representing a single M. ulcerans circulating genotype, we found instead two distinct genotypes. One genotype was closely related to isolates from neighbouring regions of Amansie West and Densu, consistent with the predicted local endemic clone, but the second genotype (separated by 138 single nucleotide polymorphisms [SNPs] from other Ghanaian strains most closely matched M. ulcerans from Nigeria, suggesting another introduction of M. ulcerans to Ghana, perhaps from that country. Both the exotic genotype and the local Ghanaian genotype displayed highly restricted intra-strain genetic variation, with less than 50 SNP differences across a 5.2 Mbp core genome within each genotype. Interestingly, there was no discernible spatial clustering of genotypes at the local village scale. Interviews revealed no obvious epidemiological links among BU patients who had been infected with identical M. ulcerans genotypes but lived in geographically separate villages. We conclude that M. ulcerans is spread widely across the region, with multiple genotypes present in any one area. These data give us new perspectives on the behaviour of possible reservoirs and subsequent transmission mechanisms of M. ulcerans. These observations also show for the first time that M. ulcerans can be mobilized, introduced to a new area and then spread within a population. Potential reservoirs of M. ulcerans

  17. Filling data gaps on the diversity and distribution of Amazonian bats (Chiroptera): the case of Amapá, easternmost Brazil

    OpenAIRE

    Ana C. M. Martins; Enrico Bernard; Renato Gregorin; Wanuyze A. S. da Silva

    2011-01-01

    Brazil is the second most bat species-rich country in the world, but the information on its species diversity, occurrence and distribution is still heterogeneous and fragmented. None of the Brazilian biomes are well surveyed for bats, but this situation is more critical in Amazonia, an area covering nearly 2/3 of the country. Here we provide updated information on the bats of Amapá, once a data gap in the diversity and distribution of bats in the easternmost Amazonia, and the Guiana Shield as...

  18. Distribution of trace elements in tissues of shrimp species Litopenaeus vannamei (Boone, 1931) from Bahia, Brazil.

    Science.gov (United States)

    Silva, E; Viana, Z C V; Onofre, C R E; Korn, M G A; Santos, V L C S

    2016-02-01

    In this study, concentrations of trace elements in tissues of shrimp species (Litopenaeus vannamei) from farming and zone natural coastal located in the northeastern Brazil were investigated. The elements determination was performed by optical emission spectrometry with inductively coupled plasma (ICP OES). The following ranges of concentrations in the tissues were obtained in µg g-1 dry weight: Al: 13.4-886.5, Cd: 0.93-1.80; Cu: 24.8-152; Fe: 3.2-410.9; Mn: 0.36-24.4; Se: 0.094-9.81 and Zn: 20.3-109.4. The shrimp muscle can be a good iron source (about 88.9 mg-1g dry weight). The distribution of Se concentration in tissues showed much variation between locations, and the concentration levels found in shrimp muscles of wild samples were high, where its levels in 67% of muscle and 50% of others tissues samples exceeded the ANVISA limit, indicating evidence of selenium bioaccumulation. Significant correlation was observed between the following pairs of elements: Fe-Zn (r= -0.70), Mn-Cu (r= -0.74), Se-Cu (r= -0.68), Se-Mn (r= 0.82) in the muscles; Fe-Al (r= 0.99), Mn-Al (r= 0.62), Mn-Fe (r= 0.62), Se-Al (r = 0.88), Se-Fe (r= 0.87), Se-Mn (r= 0.58) in the exoskeleton and Cu-Zn (r = 0.68), Al-Cu (r= 0.88), Fe-Cu (r= 0.95) and Fe-Al (r= 0.97) in the viscera. PMID:26909636

  19. Spatial distribution of triatomines (Reduviidae: Triatominae in urban areas of the city of Salvador, Bahia, Brazil

    Directory of Open Access Journals (Sweden)

    Karine de Souza O. Santana

    2011-05-01

    Full Text Available Environmental changes have a strong influence on the emergence and/or reemergence of infectious diseases. The city of Salvador, Brazil -currently the focus of a housing boom linked to massive deforestation- is an example in point as the destruction of the remaining areas of the Atlantic Forest around the city has led to an increased risk for Chagas disease. Human domiciles have been invaded by the triatomine vectors of Trypansoma cruzi, the flagellate protozoan causing Chagas disease, a problem of particular concern in urban/suburban areas of the city such as the Patamares sector in the north-east, where numbers of both the vector and human cases of the disease have increased lately. To control and prevent further deterioration of the situation, the control programme for Chagas disease, developed by the Bahia Center for Zoonosis Control, has divided the area into a grid of designated surveillance units (ZIs that are subjected to vector examination. In six out of 98 of these ZIs, 988 triatomes were collected and georeferenced during the 3-year period between 2006 and 2009. The hottest months, that are also generally the driest, showed the highest numbers of triatomines with Triatoma tibiamaculata being the predominant species (98.3% with Panstrongylus geniculatus present only occasionally (0.6%. Fifty-four percent of all triatomines captured were found inside the homes, and 48.6% out of 479 individuals in the affected ZIs selected for analysis tested positive for T. cruzi infection. The study presented here is a pioneering initiative to map the spatial distribution of triatomines based on geographical information systems with the additional aim of contributing to an expanded knowledge-base about T. cruzi and its vectors in urban areas and raise public health awareness of the risks involved.

  20. Coexistence and geographical distribution of Leguminosae in an area of Atlantic forest in the semi-arid region of Brazil

    Institute of Scientific and Technical Information of China (English)

    Jacira R. LIMA; Vidal F. MANSANO; Francisca S. ARA(U)JO

    2012-01-01

    Understanding the factors that affect plant species distribution and coexistence in areas with high plant species diversity is a challenge for ecologists.According to some authors,species occupy specific niches,but for others,species coexistence and geographical distribution patterns are random.Floristic composition of the family Leguminosae was studied on moist and dry slopes of the Baturité mountains in semi-arid northeastern Brazil and was compared with findings for other plant formations elsewhere in Brazil.Substantial floristic differences were found between the moist windward and dry leeward slopes of the Baturité mountains despite their close geographical proximity.The leeward slope was slightly more diverse than the windward slope.Similarity analyses showed that the windward face is floristically allied to the Amazon forest,whereas the leeward slope is similar to other dry-area formations of northeastern Brazil,such as thorny woodland (caatinga) and seasonal forests.The strong floristic differences that were observed between the windward and leeward slopes corroborate the theory of ecological niche conservatism,which holds that species occurrence is closely linked to environmental factors,such as temperature and precipitation.

  1. Genotypic distribution and phylogenetic characterization of Enterocytozoon bieneusi in diarrheic chickens and pigs in multiple cities, China: potential zoonotic transmission.

    Directory of Open Access Journals (Sweden)

    Wei Li

    Full Text Available This study investigated diarrheic broiler and layer chickens (60 days; n=64 for E. bieneusi genotypes in northeast China and evaluated the potential roles of chickens and pigs in zoonotic transmission of microsporidiosis. Two 45-day-old layer chickens in city Jixi, Heilongjiang province and one 23-day-old broiler chicken in city Songyuan, Jilin province were identified to harbor a human-pathogenic E. bieneusi genotype Henan-IV and a new genotype named CC-1, respectively, by nested PCR and sequence analysis of the ribosomal internal transcribed spacer (ITS. Eleven of 64 (17.2% duodenal mucosal specimens from pigs in city Tianjin, city Tongliao of Inner Mongolia, cities Jilin and Songyuan of Jilin province, and cities Daqing, Harbin, and Suihua of Heilongjiang province, were positive for E. bieneusi, with the infection rates of weaned pigs (35%, 7/20 significantly higher than preweaned ones (3.6%, 1/28; P<0.05. Nucleotide sequences of the ITS were obtained from 6 pig specimens, belonging to 3 known genotypes CHN7, EbpC, and Henan-IV. That the previous reports have described the occurrence of genotypes EbpC and Henan-IV in humans and EbpC in wastewater in central China and the clustering of genotypes CC-1 and CHN7 into a major phylogenetic group of E. bieneusi genotypes with zoonotic potential indicated that chickens and pigs could be potential sources of human micorsporidiosis. To our knowledge, this is the first report describing the existence of zoonotic E. bieneusi genotypes in diarrheic chickens.

  2. Selection of common bean (Phaseolus vulgaris L.) genotypes using a genotype plus genotype x environment interaction biplot.

    Science.gov (United States)

    Corrêa, A M; Teodoro, P E; Gonçalves, M C; Santos, A; Torres, F E

    2016-01-01

    Recently, the genotype plus genotype x environment interaction (GGE) biplot methodology has been used to investigate genotype x environment interactions in several crop species, but has not been applied to the common bean (Phaseolus vulgaris L.) crop in Brazil. The aim of this study was to identify common bean genotypes that exhibit high grain yield and stability in the State of Mato Grosso do Sul, Brazil. We conducted 12 trials from 2000 to 2006 in the municipalities of Aquidauana and Dourados, and evaluated 13 genotypes in a randomized block design with three replications. Grain yield data were subjected to individual and joint analyses of variance. After analyzing the GE interaction, the adaptability and phenotypic stability of the common bean genotypes were analyzed using GGE biplot methodology. The genotypes EMGOPA-201, Xamego, and Aporé are recommended for growing in Mato Grosso do Sul, because they exhibited high grain yield and phenotypic stability. PMID:27525915

  3. Spatial urban restructuring for economic growth with distribution: The case of Ceara (Brazil)

    OpenAIRE

    Raphael BAR-EL; Schwartz, Dafna

    2003-01-01

    One of the most important challenges of economic policy is the combination between economic growth and the reduction of inequality and poverty. The change of the regime in Brazil can be seen as a reaction to the failure of economic policy in this field. We focus on the case of one of Brazil's states in the Northeast, Ceara, that experienced a rapid macro economic growth in the last decade, but with no reduction of poverty and inequality. Established economic theory shows that economic growth ...

  4. Perilestes eustaquioi sp. nov. and new distributional records of Perilestidae (Odonata in Brazil

    Directory of Open Access Journals (Sweden)

    Angelo B. M. Machado

    2015-10-01

    Full Text Available ABSTRACT Perilestes eustaquioi sp. nov. (holotype male deposited in ABMM collection from the state of Bahia (municipality of Una, northeastern Brazil, is described and illustrated based on one male specimen. It differs from the other species of the genus mainly by the larger size of the anteclypeus in relations to the postclypeus. Together with P. fragilis Hagen in Selys, 1862 from the state of Sergipe and P. solutus Williamson & Williamson, 1924 from the state of Ceará, these are the first records of Perilestidae from northeastern Brazil.

  5. Distribution of genotypes C825T polymorphism G-protein β3-subunit gene in patients with hypertension depending on body mass index

    Directory of Open Access Journals (Sweden)

    Prystupa L.N.

    2015-09-01

    Full Text Available The aim of the study was to investigate the frequency of genotypes of C825T polymorphism G-protein β3-subunit gene (GNB3 in patients with arterial hypertension (AH, depending on body mass index (BMI. The study involved 155 patients with verified diagnosis of AH (study group and 50 healthy individuals (control group. The patients of the main group were divided into 3 groups according to BMI: I - 35 patients with normal body weight, II - 38 patients with overweight, III - 82 patients with obesity. We used general clinical, anthropometric, instrumental, molecular-genetic and statistical methods. Probability of differences in the frequency of alleles and genotypes was determined using χ² criteria. Pairwise comparison of groups was made using nonparametric Mann-Whitney test. The difference was considered statistically significant at p <0,05. Investigation of the distribution of genotypes C825T polymorphism GNB3 in patients with AH according to BMI showed statistically significant increase in the frequency of genotypes C / T and T / T and T allele in patients with overweight and obesity as compared with patients with normal body weight (χ² = 26 8; p <0.001. The risk of weight increase in AH patients with T allele carriers is 2,2 times higher than in C allele carriers. Association of C825T polymorphism of GNB3 with a tendency to obesity and overweight in patients with AH was proved.

  6. Absence of Helicobacter pylori high tetracycline resistant 16S rDNA AGA926-928TTC genotype in gastric biopsy specimens from dyspeptic patients of a city in the interior of São Paulo, Brazil

    Directory of Open Access Journals (Sweden)

    Suzuki Rodrigo

    2012-05-01

    Full Text Available Abstract Background Treatment effectiveness of Helicobacter pylori varies regionally and is decreasing worldwide, principally as a result of antibiotic resistant bacterium. Tetracycline is generally included in second line H. pylori eradication regimens. In Brazil, a high level of tetracycline resistance (TetR is mainly associated with AGA926-928TTC 16 S rDNA nucleotide substitutions. As H. pylori culture is fastidious, we investigated the primary occurrence of H. pylori 16 S rDNA high level TetR genotype using a molecular approach directly on gastric biopsies of dyspeptic patients attending consecutively at Hospital das Clinicas of Marilia, São Paulo, Brazil. Methods Gastric biopsy specimens of 68 peptic ulcer disease (PUD and 327 chronic gastritis (CG patients with a positive histological diagnosis of H. pylori were investigated for TetR 16 S rDNA genotype through a molecular assay based on amplification of a 16 S rDNA 545 bp fragment by polymerase chain reaction and HinfI restriction fragment length polymorphism (PCR/RFLP. Through this assay, AGA926-928TTC 16 S rDNA TetR genotype resulted in a three DNA fragment restriction pattern (281, 227 and 37 bp and its absence originated two DNA fragments (264 and 281 bp due to a 16 S rDNA conserved Hinf I restriction site. Results The 545 bp 16 S rDNA PCR fragment was amplified from 90% of gastric biopsies from histological H. pylori positive patients. HinfI RFLP revealed absence of the AGA926–928TTC H. pylori genotype and PCR products of two patients showed absence of the conserved 16 S rDNA HinfI restriction site. BLASTN sequence analysis of four amplicons (two conserved and two with an unpredicted HinfI restriction pattern revealed a 99% homology to H. pylori 16 S rDNA from African, North and South American bacterial isolates. A nucleotide substitution abolished the conserved HinfI restriction site in the two PCR fragments with unpredicted HinfI RFLP, resulting in an

  7. Influence of agricultural biomass burning on aerosol size distribution and dry deposition in southeastern Brazil.

    Science.gov (United States)

    Rocha, Gisele O; Allen, Andrew G; Cardoso, Arnaldo A

    2005-07-15

    The size distributed composition of ambient aerosols is used to explore seasonal differences in particle chemistry and to show that dry deposition fluxes of soluble species, including important plant nutrients, increase during periods of biomass (sugar cane trash) burning in São Paulo State, Brazil. Measurements were made at a single site centrally located in the State's sugar cane growing region but away from the immediate vicinity of burns, so that the airsampled was representative of the regional background. Calculation of ion equivalent balances showed that during burning periods smaller particles (Aitken and accumulation modes) were more acidic, containing higher concentrations of SO4(2-), oxalate, NO3-, HCOO-, CH3COO-, and CI-, but insufficient NH4+ and K+ to achieve neutrality. Larger particles showed an anion deficit due to the presence of unmeasured ions and comprised resuspended dusts modified by accumulation of nitrate, chloride, and organic anions. Increases of resuspended particles during the burning season were attributed to release of earlier deposits from the surfaces of burning vegetation as well as increased vehicle movement on unsurfaced roads. During winter months the relative contribution of combined emissions from road transport and industry diminished due to increased emissions from biomass combustion and other activities specifically associated with the harvest period. Positive increments in annual particulate dry deposition fluxes due to higher fluxes during the sugar cane harvest were 44.3% (NH4+), 42.1% (K+), 31.8% (Mg2+), 30.4% (HCOO-), 12.8% (CI-), 6.6% (CH3COO-), 5.2% (Ca2+), 3.8% (SO4(2-)), and 2.3% (NO3-). Na+ and oxalate fluxes were seasonally invariant. Annual aerosol dry deposition fluxes (kg ha(-1)) were 0.5 (Na+), 0.25 (NH4+), 0.39 (K+), 0.51 (Mg2+), 3.19 (Ca2+), 1.34 (Cl-), 4.47 (NO3-), 3.59 (SO4(2-)), 0.58 (oxalate), 0.71 (HCOO-), and 1.38 (CH3COO-). Contributions of this mechanism to combined aerosol dry deposition and

  8. Studies on chaetognaths off Ubatuba region, Brazil: I. distribution and abundance

    Directory of Open Access Journals (Sweden)

    Liang Tsui Hua

    1994-12-01

    Full Text Available The distribution of chaetognath species off Ubatuba region, São Paulo State, Brazil, was studied during a program of multidisciplinary research. Ten species belonging to the genera Sagitta, Krohnita and Pterosagitta were identified. S. enflata was the dominant species followed by S.friderici and S. hispida. The species S. enflata, S. hispida, S. tenuis, S. bipunctata and JC pacifica were found in the Shelf water whereas S. serratodentata, S. minima, S. hexaptera and P. draco in the Tropical water. Only S. friderici was found associated to Coastal water. Hydrological conditions affected population structure, size of individuals and abundance.A ocorrência, distribuição, freqüência dos estágios de maturidade e comprimento total do corpo das espécies do filo Chaetognatha foram estudados. As amostras foram obtidas com o auxílio de rede Bongo, nos verões de 1985 - 1987 e invernos de 1986 e 1987, durante o Projeto "Utilização Racional do Ecossistema Costeiro da Região Tropical Brasileira, Estado de São Paulo". Dez espécies foram identificadas, sendo Sagitta enflata, S. friderici e S. hispida as espécies mais abundantes. S. enflata, S. hispida, S. tenuis, S. bipunctata e Krohnita pacifica estão associadas à água de Plataforma enquanto que S. serratodentata, S. hexaptera, S. minima e Pterosagitta draco à água Tropical. Apenas S. friderici mostrou preferência por água Costeira. Diferenças sazonais na estrutura da população, tamanho dos indivíduos, abundância e distribuição estão associados à hidrodinâmica local. Nos verões, os quetognatos apresentaram maior número de estágios maduros nas amostras examinadas, comprimentos maiores e baixa abundância como conseqüência da intrusão da água Central do Atlântico Sul que provocou uma estratificação térmica característica. Em contraposição, nestas amostras, nos invernos, a população é formada por indivíduos de estágios jovens, de comprimentos menores e grande

  9. Mapping Fractional Cropland Distribution in Mato Grosso, Brazil Using Time Series MODIS Enhanced Vegetation Index and Landsat Thematic Mapper Data

    Directory of Open Access Journals (Sweden)

    Changming Zhu

    2015-12-01

    Full Text Available Mapping cropland distribution over large areas has attracted great attention in recent years, however, traditional pixel-based classification approaches produce high uncertainty in cropland area statistics. This study proposes a new approach to map fractional cropland distribution in Mato Grosso, Brazil using time series MODIS enhanced vegetation index (EVI and Landsat Thematic Mapper (TM data. The major steps include: (1 remove noise and clouds/shadows contamination using the Savizky–Gloay filter and temporal resampling algorithm based on the time series MODIS EVI data; (2 identify the best periods to extract croplands through crop phenology analysis; (3 develop a seasonal dynamic index (SDI from the time series MODIS EVI data based on three key stages: sowing, growing, and harvest; and (4 develop a regression model to estimate cropland fraction based on the relationship between SDI and Landsat-derived fractional cropland data. The root mean squared error of 0.14 was obtained based on the analysis of randomly selected 500 sample plots. This research shows that the proposed approach is promising for rapidly mapping fractional cropland distribution in Mato Grosso, Brazil.

  10. Privatization of electricity distribution in the Northeast of Brazil: The good, the bad, the ugly or the naive?

    International Nuclear Information System (INIS)

    This paper analyzes the evolution of five electricity distribution companies in the Northeast of Brazil using technical and financial indicators. Three privatized and two public firms were analyzed between 1997 and 2008. The financial indicators are used as proxies for the capacity of the business to generate value for shareholders, while the technical indicators are used as proxies for service quality provided to consumers. We observed that the privatized firms had their financial indicators improved after privatization, increasing the value of the firm for the shareholders. However, there is no evidence that privatization affected the quality of service provided to consumers.

  11. Temporal distribution of five bat species (Chiroptera, Phyllostomidae from Panga Reserve, south-eastern Brazil

    Directory of Open Access Journals (Sweden)

    Wagner A. Pedro

    2002-09-01

    Full Text Available Data about activity patterns, hourly and monthly, on five phyllostomid bats, Glossophaga soricina (Pallas, 1766, Anoura caudifer (E. Geoffroy, 1818, Camilla perspicillata (Linnaeus, 1758, Sturnira lilium (E. Geoffroy, 1810 and Platyrrhinus lineatus (E. Geoffroyi, 1810, studied over a one year period at the Panga Ecological Reserve, Uberlandia, Minas Gerais state, south-eastern Brazil, are reported and discussed.

  12. Temporal distribution of five bat species (Chiroptera, Phyllostomidae) from Panga Reserve, south-eastern Brazil

    OpenAIRE

    Wagner A Pedro; Valdir A. Taddei

    2002-01-01

    Data about activity patterns, hourly and monthly, on five phyllostomid bats, Glossophaga soricina (Pallas, 1766), Anoura caudifer (E. Geoffroy, 1818), Camilla perspicillata (Linnaeus, 1758), Sturnira lilium (E. Geoffroy, 1810) and Platyrrhinus lineatus (E. Geoffroyi, 1810), studied over a one year period at the Panga Ecological Reserve, Uberlandia, Minas Gerais state, south-eastern Brazil, are reported and discussed.

  13. Fuel distribution logistics in Brazil: technical and economic aspects; Aspectos tecnico-economicos da logistica da distribuicao de combustiveis no Brasil

    Energy Technology Data Exchange (ETDEWEB)

    Esteves, Heloisa Borges B.; Bicalho, Lucia N. [Agencia Nacional do Petroleo, Gas Natural e Biocombustiveis (ANP), Rio de Janeiro, RJ (Brazil)

    2008-07-01

    In Brazil, there are around 250 companies operating in the automotive fuel distribution segment, responsible for the automotive fuels wholesale commercialization activity. Those companies supply not only gas stations, but also final consumers and TRR's. Their clients are distributed regionally, which requires flexibility and the ability to supply clients efficiently at the smaller possible cost, transforming variables such as the transportation alternatives adopted, the location of the storage facilities and its correct dimension on key decisions. This paper analyses the general scenario of the fuels distribution logistics in Brazil from a economic perspective, and its impacts on the companies competitive strategies. (author)

  14. Seasonal chaetognath abundance and distribution in a tropical estuary (Southeastern, Brazil

    Directory of Open Access Journals (Sweden)

    Luiz Loureiro Fernandes

    2005-06-01

    Full Text Available This study focuses on the seasonal variation of the chaetognath species in the Vitória Bay/Passage Channel estuarine system, Espírito Santo, Brazil, in terms of their abundance and distribution. Specimens of chaetognaths were collected between July 1997 and April 1998 at 10 sampling stations, with a cylindrical-conical plankton net of 200 µm mesh and 30 cm mouth, fitted with a mechanical flowmeter. Five chaetognath species were identified: Sagitta enflata, Sagitta decipiens, Sagitta hispida, Sagitta friderici and Sagitta minima. Most of them were distributed in areas of high salinity (e.g. at the stations closest to the outer estuary. The dominant species, S. enflata and S. friderici, were more frequent in the outer estuary where salinities varied from 32 (wet season - summer to 28 (dry season - winter. S. friderici was the only species found right in the middle of the Passage Channel, at a station close to the main freshwater input into the estuary. Results showed that chaetognaths only enter the estuary due to the tidal effect, and that they are not typical residents of this system. This is to be expected because the group normally inhabits only truly marine regions.Este estudo abordou a abundância e distribuição sazonal das espécies de quetognatos, no sistema estuarino baía de Vitória/Canal da Passagem, Espírito Santo, Brasil. Os organismos foram coletados entre julho de 1997 e abril de 1998 em 10 estações amostrais, utilizando uma rede de plâncton cilíndrico-cônica de 200µm de malha e 30 cm de abertura de boca, dotada de um fluxômetro mecânico. Cinco espécies de quetognatos foram identificadas: Sagitta enflata, Sagitta decipiens, Sagitta hispida, Sagitta friderici e Sagitta minima. A maioria destas espécies esteve distribuída em áreas com alta salinidade (e.g. estações próximas a saída do estuário. As espécies dominantes S. enflata e S. friderici foram mais freqüentes na parte externa do estuário onde as

  15. Estabilidade fenotípica de genótipos de algodoeiro no Estado do Mato Grosso Phenotypic stability in cotton genotypes in Mato Grosso State, Brazil

    Directory of Open Access Journals (Sweden)

    Eulália Soler Sobreira Hoogerheide

    2007-05-01

    Full Text Available Foram avaliados oito genótipos de algodoeiro herbáceo, sendo três linhagens e cinco cultivares, com o objetivo de estimar a adaptabilidade e estabilidade fenotípica para o caráter produtividade de algodão em caroço, pelo método Eberhart e Russell. Foram conduzidos 12 experimentos em 11 locais no Estado do Mato Grosso, sob um delineamento de blocos ao acaso com oito repetições, no ano agrícola 2000/2001. Praticamente todos os genótipos apresentaram coeficiente de determinação acima de 85%, exceto Delta Opal. As estimativas de adaptabilidade indicam que todos os genótipos apresentaram adaptação ampla (b i = 1. Quanto à estabilidade, os genótipos CNPA ITA 90, BRS Antares, CNPA 96-124, CNPA 96-283 e BRS Aroeira revelaram-se estáveis (S²d i= 0. Os melhores genótipos, caracterizados pela maior produtividade, estabilidade e adaptabilidade ampla foram CNPA ITA 90, BRS Aroeira e CNPA 96-124.Eight cotton genotypes, three lines and five cultivars, were evaluated for estimation of phenotypic adaptability and stability parameters relative to cotton yield using the method proposed by Eberhart and Russell. Twelve yield trials, in randomized complete blocks, comprising eight replications, were carried out in 11 locations of the Mato Grosso State, during the 2000/2001 crop season. All the genotypes showed determination coefficient above of 85%, except Delta Opal. For the estimates of adaptability, all the genotypes presented broad adaptation (b i = 1. The genotypes CNPA ITA 90, BRS Antares, CNPA 96-124, CNPA 96-283 and BRS Aroeira showing hight stability (S²d i= 0. The best genotypes, characterized by higher yield, stability and broad adaptability, were CNPA ITA 90, BRS Aroeira and CNPA 96-124.

  16. The diversity and distribution of Heliconia (Heliconiaceae in Brazil A diversidade e distribuição de Heliconia (Heliconiaceae

    Directory of Open Access Journals (Sweden)

    John Kress

    1990-07-01

    Full Text Available A review of the phylogenetic relationships of the Zingiberales is presented and the distribution patterns and diversity of Heliconia are discussed. Sixty-five species names have been applied to the heliconias that occur in Brazil. Of these 65 names, 28 are generally recognized synonyms. Of the remaining 37 species of Brazilian Heliconia, some controversy exists over the taxonomic status of at least eight. Two primary areas of distribution of species of Heliconia exist in Brazil: the Amazon basin (21 species and the Atlantic coastal forest (20 species.É apresentada uma revisão filogenética das Zingiberales e os padrões de distribuição geográfica e a diversidade das Heliconia são apresentados. Para o gênero são referidos 65 nomes de espécies dos quais provavelmente 28 sejam sinonímias. Das 37 espécies restantes, existe controvérsia pelo menos para oito delas. Duas áreas de distribuição de espécies de Heliconia são apresentadas para o Brasil: a bacia amazônica (21 espécies e a floresta costeira Atlântica (20 espécies.

  17. Spatial-temporal distribution of fire-protected savanna physiognomies in Southeastern Brazil

    Directory of Open Access Journals (Sweden)

    Marcelo H.O. Pinheiro

    2010-06-01

    Full Text Available The analysis of the influence of edaphic finer textures, as a facilitating factor for the expansion of forest formations in the absence of fire, was possible thanks to rare characteristics found in a savanna fragment located in the State of São Paulo, Brazil. The total suppression of fire for over four decades, and the occurrence of two savanna physiognomies, cerrado sensu stricto and cerradão, allowed the conduction of this study based on the hypothesis that cerradão, a physiognomy of forest aspect consisting of fire-sensitive tree and shrubs species, is favored by fire absence and higher soil hydric retention capacity. Edaphic samples were collected from a regular grid of 200 m² for the production of isopletic maps of the distribution of clay, fine sand, coarse sand and silt edaphic textures by the geostatistic method of ordinary kriging. Changes in the areas occupied by both savanna physiognomies, defined on the basis of aerial photographs taken over a period of 43 years, were assessed through mean variation rates. Besides corroborating the hypothesis of edaphic hydric retention as a facilitating factor for the expansion of forest physiognomies in savanna areas, we were able to infer the positive influence of higher precipitation on the increase in cerradão expansion rates.A influência de texturas edáficas finas, como fator de facilitação para a expansão de formações florestais sobre áreas savânicas, através da maior retenção hídrica edáfica, na ausência de incêndios, foi possível ser estudada graças às características encontradas em um fragmento savânico com 38,8 ha, situado em Corumbataí (SP. A supressão total do fogo por quatro décadas, e a ocorrência de duas fisionomias, cerrado sensu stricto e cerradão, permitiram a condução deste estudo. Amostras de solo foram coletadas em uma grade regular de 200 m², abrangendo toda a área do fragmento. Foram produzidos mapas iso-pléticos, com a distribuição das

  18. Spatial distribution of dengue incidence and socio-environmental conditions in Campinas, São Paulo State, Brazil, 2007.

    Science.gov (United States)

    Costa, José Vilton; Donalisio, Maria Rita; Silveira, Liciana Vaz de Arruda

    2013-08-01

    This study aimed to analyze the spatial distribution of dengue risk and its association with socio-environmental conditions. This was an ecological study of the counts of autochthonous dengue cases in the municipality of Campinas, São Paulo State, Brazil, in the year 2007, aggregated according to 47 coverage areas of municipal health centers. Spatial models for mapping diseases were constructed with Bayesian hierarchical models, based on Integrated Nested Laplace Approximation (INLA). The analyses were stratified according to two age groups, 0 to 14 years and above 14 years. The results indicate that the spatial distribution of dengue risk is not associated with socio-environmental conditions in the 0 to 14 year age group. In the age group older than 14 years, the relative risk of dengue increases significantly as the level of socio-environmental deprivation increases. Mapping of socio-environmental deprivation and dengue cases proved to be a useful tool for data analysis in dengue surveillance systems.

  19. Phenotypic and genotypic characteristics of shiga toxin-producing Escherichia coli strains isolated from children in São Paulo, Brazil

    OpenAIRE

    Guth Beatriz EC; Ramos Sônia RTS; Cerqueira Aloysio MF; Andrade João RC; Gomes Tânia AT

    2002-01-01

    The biochemical and serological characteristics, virulence properties, and genetic relatedness of Shiga toxin-producing Escherichia coli (STEC) strains isolated in São Paulo, from April 1989 through March 1990, were determined. This is also the first report on clinic findings of human STEC infections in Brazil. The only three STEC strains identified in that period were lysine decarboxylase negative, belonged to serotype O111ac: non-motile, were Stx1 producers, carried the eae and astA genes, ...

  20. Susceptibility of biallelic haplotype and genotype frequencies to genotyping error.

    Science.gov (United States)

    Moskvina, Valentina; Schmidt, Karl Michael

    2006-12-01

    With the availability of fast genotyping methods and genomic databases, the search for statistical association of single nucleotide polymorphisms with a complex trait has become an important methodology in medical genetics. However, even fairly rare errors occurring during the genotyping process can lead to spurious association results and decrease in statistical power. We develop a systematic approach to study how genotyping errors change the genotype distribution in a sample. The general M-marker case is reduced to that of a single-marker locus by recognizing the underlying tensor-product structure of the error matrix. Both method and general conclusions apply to the general error model; we give detailed results for allele-based errors of size depending both on the marker locus and the allele present. Multiple errors are treated in terms of the associated diffusion process on the space of genotype distributions. We find that certain genotype and haplotype distributions remain unchanged under genotyping errors, and that genotyping errors generally render the distribution more similar to the stable one. In case-control association studies, this will lead to loss of statistical power for nondifferential genotyping errors and increase in type I error for differential genotyping errors. Moreover, we show that allele-based genotyping errors do not disturb Hardy-Weinberg equilibrium in the genotype distribution. In this setting we also identify maximally affected distributions. As they correspond to situations with rare alleles and marker loci in high linkage disequilibrium, careful checking for genotyping errors is advisable when significant association based on such alleles/haplotypes is observed in association studies.

  1. Prenatal pesticide exposure and PON1 genotype associated with adolescent body fat distribution evaluated by dual X-ray absorptiometry (DXA).

    Science.gov (United States)

    Tinggaard, J; Wohlfahrt-Veje, C; Husby, S; Christiansen, L; Skakkebaek, N E; Jensen, T K; Grandjean, P; Main, K M; Andersen, H R

    2016-07-01

    Many modern pesticides have endocrine disrupting abilities and early-life exposure may affect growth and disease risk later in life. Previously, we reported associations between prenatal pesticide exposure and higher childhood body fat content measured by anthropometry. The associations were affected by child PON1 Q192R genotype. We aimed to study whether prenatal pesticide exposure was still associated with body fat content and distribution in the children at puberty and the potential impact of both maternal and child PON1 Q192R genotype. In this prospective cohort study of 247 children born by occupationally exposed or unexposed women (greenhouse workers and controls) two follow-up examinations (age 10-15 and 11-16 years) including simple anthropometry, skinfold measurements, pubertal staging and blood sampling were performed. Total and regional fat% was determined by dual X-ray absorptiometry (DXA) at age 10-15. Prenatal pesticide exposure was associated with increased total, android, and gynoid fat percentage (DXA) at age 10-15 years after adjustment for sex, socioeconomic status, and puberty (all β = 0.5 standard deviation score (SDS) p android-gynoid ratio: β = 0.1, both p android fat deposition, independent of puberty. Girls appeared more susceptible than boys. Furthermore, the association depended on maternal and child PON1 Q192R genotype.

  2. Stability and adaptability of runner peanut genotypes based on nonlinear regression and AMMI analysis

    Directory of Open Access Journals (Sweden)

    Roseane Cavalcanti dos Santos

    2012-08-01

    Full Text Available The objective of this work was to estimate the stability and adaptability of pod and seed yield in runner peanut genotypes based on the nonlinear regression and AMMI analysis. Yield data from 11 trials, distributed in six environments and three harvests, carried out in the Northeast region of Brazil during the rainy season were used. Significant effects of genotypes (G, environments (E, and GE interactions were detected in the analysis, indicating different behaviors among genotypes in favorable and unfavorable environmental conditions. The genotypes BRS Pérola Branca and LViPE‑06 are more stable and adapted to the semiarid environment, whereas LGoPE‑06 is a promising material for pod production, despite being highly dependent on favorable environments.

  3. Simulation of Distributed Generation with Photovoltaic Microgrids—Case Study in Brazil

    Directory of Open Access Journals (Sweden)

    Gustavo Azevedo Xavier

    2015-05-01

    Full Text Available Elevated prices and lack of proper legislation and government incentives have been the main barriers in the development of the photovoltaic market in Brazil. In an attempt to overcome those barriers, a microgrid model was proposed and simulated. In the proposed microgrids, residential consumers are connected to each other to maximize the investment return by trading the surplus of generated energy among them. Different topologies and scenarios were studied from electrical energy and economic standpoints. Stochastic data of solar radiation were simulated for the city of Viçosa, Minas Gerais, Brazil, for the period of one year, considering the statistical behavior of a series over 20 years. The system output power and energy balance were calculated considering a model for photovoltaic generators and the radiation simulated data. By determining the generated energy and electrical needs of the microgrid members, the cash flow and economic feasibility were calculated. Sensitivity analyses were performed by varying economic parameters to determine situations where investment becomes feasible. This paper shows that microgrid contributes to improve the economics and the initial investments. The number of participants in a microgrid, the electricity and the equipment costs are important parameters to speed up the economic and technical feasibility process.

  4. Populational fluctuation and spatial distribution of Alphitobius diaperinus (Panzer) (Coleoptera; Tenebrionidae) in a poultry house, Cascavel, Parana state, Brazil.

    Science.gov (United States)

    Chernaki-Leffer, A M; Almeida, L M; Sosa-Gómez, D R; Anjos, A; Vogado, K M

    2007-05-01

    Knowledge of the population fluctuation and spatial distribution of pests is fundamental for establishing an appropriate control method. The population fluctuation and spatial distribution of the Alphitobius diaperinus in a poultry house in Cascavel, in the state of Parana, Brazil, was studied between October, 2001 and October 2002. Larvae and adults of the lesser mealworm were sampled weekly using Arends tube traps (n = 22) for six consecutive flock grow-outs. The temperature of the litter and of the poultry house was measured at the same locations of the tube traps. Beetle numbers increased continuously throughout all the sampling dates (average 5,137 in the first week and 18,494 insects on the sixth week). Significantly greater numbers of larvae were collected than adults (1 to 20 times in 95% of the sampling points). There was no correlation between temperature and the number of larvae and adults collected, therefore no fluctuation was observed during the sampling period. The population growth was correlated to litter re-use. The highest temperatures were observed in deep litter. The spatial distribution of larvae and adults in the poultry house was heterogeneous during the whole period of evaluation. Results suggest that monitoring in poultry houses is necessary prior to adopting and evaluating control measures due to the great variability of the insect distribution in the poultry house. PMID:17876430

  5. Monitoring and spatial distribution of heterotrophic bacteria and fecal coliforms in the Rodrigo de Freitas Lagoon, Rio de Janeiro, Brazil

    Directory of Open Access Journals (Sweden)

    Lutterbach Márcia T. S.

    2001-01-01

    Full Text Available The distribution of heterothrophic bacteria and fecal coliforms was monitored at four sampling stations located near the shore of the Rodrigo de Freitas Lagoon, in the city of Rio de Janeiro, Brazil. Water samples were collected, monthly from October 1994 through September 1998. The highest heterothrophic count (6.5x10 7 CFU/100mL was recorded at stations 2 and 4 during August 1998 and the lowest (10 ³ CFU/100 mL at station 3 during February 1995. With respect to fecal coliforms, the highest and lowest counts were 1.6x10 5 coliforms/100mL at station 3 during March 1997 and <1 coliform/100mL at all the stations during February 1995 and September 1997 as well as station 3 during February 1998. The data indicated a percentage increase of the microorganisms surveyed over time at all the sampling stations studied.

  6. RAD genotyping reveals fine-scale genetic structuring and provides powerful population assignment in a widely distributed marine species, the American lobster (Homarus americanus).

    Science.gov (United States)

    Benestan, Laura; Gosselin, Thierry; Perrier, Charles; Sainte-Marie, Bernard; Rochette, Rémy; Bernatchez, Louis

    2015-07-01

    Deciphering genetic structure and inferring connectivity in marine species have been challenging due to weak genetic differentiation and limited resolution offered by traditional genotypic methods. The main goal of this study was to assess how a population genomics framework could help delineate the genetic structure of the American lobster (Homarus americanus) throughout much of the species' range and increase the assignment success of individuals to their location of origin. We genotyped 10 156 filtered SNPs using RAD sequencing to delineate genetic structure and perform population assignment for 586 American lobsters collected in 17 locations distributed across a large portion of the species' natural distribution range. Our results revealed the existence of a hierarchical genetic structure, first separating lobsters from the northern and southern part of the range (FCT  = 0.0011; P-value = 0.0002) and then revealing a total of 11 genetically distinguishable populations (mean FST  = 0.00185; CI: 0.0007-0.0021, P-value genetic structure. We discuss the implications of these findings for the conservation and management of highly connected marine species, particularly regarding the geographic scale of demographic independence.

  7. Key locations for soybean genotype assessment in Central Brazil Locais-chave para avaliação de genótipos de soja na Região Central do Brasil

    Directory of Open Access Journals (Sweden)

    Roberto Miranda Pacheco

    2009-05-01

    Full Text Available The objective of this work was to identify key locations for the establishment of soybean (Glycine max genetic breeding programs, in the Central Region of Brazil. Grain yield data of three maturity groups of soybean genotypes, from regional trials conducted over three years, at 18 locations in Brazilian Cerrado were used. A key location for the early phases of the breeding program was defined as the site that best classifies the winning genotypes in the region. Key locations for the final phases were defined as those sites that best represent each environmental stratum, in relation to the adaptability of the respective winning genotype. This adaptability was estimated by additive main effects and multiplicative interaction (AMMI model analysis, using the distance between the score of each location in a stratum and the score of the winning genotype, which characterizes such stratum in an AMMI biplot. The locations that best classified the winning genotypes over space and time were Mineiros, Placas and Rio Verde. For the final phases of genotype selection, with data from the three maturity group, the recommended locations were: Buritis, Chapadão do Céu, Iraí, Pamplona, Placas, Planaltina, Rio Verde, Sacramento, Senador Canedo, Uberaba, and Uberlândia.O objetivo deste trabalho foi identificar locais-chave para o estabelecimento de programas de melhoramento genético de soja (Glycine max, na Região Central do Brasil. Foram utilizados dados de produtividade de grãos de genótipos de soja, de três ciclos de maturação, obtidos de ensaios regionais conduzidos por três anos em 18 localidades da região. O local-chave para a condução das fases preliminares do programa foi definido como a localidade que melhor classifica os genótipos vencedores na região. Os locais-chave para as fases finais foram definidos como os que melhor representam cada estrato ambiental identificado, em termos da adaptabilidade do respectivo genótipo vencedor. Essa

  8. Nuclear medicine annual external occupational dose distribution: Rio de Janeiro, Brazil, year 2005.

    Science.gov (United States)

    Mauricio, Claudia L P; Lima, Ana L S; da Silva, Herica L R; Souza-Santos, Denison; Silva, Claudio R

    2011-03-01

    Brazil has about 300 nuclear medicine services (NMS), 44 of them located in the state of Rio de Janeiro (RJ). Most nuclear medicine staff are routinely monitored for external dose. This paper makes a statistical analysis of all the RJ NMS annual external occupational doses in year 2005. Around 100 professionals of RJ NMS received annual doses >4.0 mSv, considering only external doses, but no one receives doses higher than the mean annual dose limit of 20 mSv. Extremities dosemeters are used by about 10 % of the staff. In some cases, these doses are more than 10 times higher than the dose in thorax. The maximum ratio of extremity dose/thorax dose, in 2005, was 72. This study shows the importance to improve radiation protection procedures in nuclear medicine, mainly because the number of occupational individuals in nuclear medicine and their external doses are increasing. PMID:21051433

  9. [The growth and distribution of the population of Brazil: recent trends].

    Science.gov (United States)

    Martine, G; Camargo, L

    1984-01-01

    Population trends underwent profound changes in Brazil during the last few decades. An important decrease in the rate of population growth was registered in the 1970s. This decrease is attributable to a decline in the level of fertility, which was observed in all regions and in both urban and rural areas. In order to explain this decline, it is necessary to analyze both structural and circumstantial factors related to the political, economic, and social context of the times. Main trends in population redistribution during the 1970s can be regrouped in terms of interregional exchanges and rural-urban migration. A growing convergence between these 2 types of patterns is observed in that population is increasingly being concentrated in densely populated areas of intense economic activities. The implications of these trends for public action are analyzed in the final section.

  10. CagA phosphorylation EPIYA-C motifs and the vacA i genotype in Helicobacter pylori strains of asymptomatic children from a high-risk gastric cancer area in northeastern Brazil

    Science.gov (United States)

    Braga, Lucia Libanez Bessa Campelo; de Oliveira, Maria Aparecida Alves; Gonçalves, Maria Helane Rocha Batista; Chaves, Fernando Kennedy; Benigno, Tiago Gomes da Silva; Gomes, Adriana Dias; Silva, Cícero Igor Simões Moura; Anacleto, Charles; Batista, Sérgio de Assis; Queiroz, Dulciene Maria Magalhães

    2014-01-01

    Helicobacter pylori infection is one of the most common infections worldwide and is associated with gastric diseases. Virulence factors such as VacA and CagA have been shown to increase the risk of these diseases. Studies have suggested a causal role of CagA EPIYA-C in gastric carcinogenesis and this factor has been shown to be geographically diverse. We investigated the number of CagA EPIYA motifs and the vacA i genotypes in H. pylori strains from asymptomatic children. We included samples from 40 infected children (18 females and 22 males), extracted DNA directly from the gastric mucus/juice (obtained using the string procedure) and analysed the DNA using polymerase chain reaction and DNA sequencing. The vacA i1 genotype was present in 30 (75%) samples, the i2 allele was present in nine (22.5%) samples and both alleles were present in one (2.5%) sample. The cagA-positive samples showed distinct patterns in the 3’ variable region of cagA and 18 of the 30 (60%) strains contained 1 EPIYA-C motif, whereas 12 (40%) strains contained two EPIYA-C motifs. We confirmed that the studied population was colonised early by the most virulent H. pylori strains, as demonstrated by the high frequency of the vacA i1 allele and the high number of EPIYA-C motifs. Therefore, asymptomatic children from an urban community in Fortaleza in northeastern Brazil are frequently colonised with the most virulent H. pylori strains. PMID:25494468

  11. Vertical and time distribution of Diplopoda (Arthropoda: Myriapoda in a monodominant forest in Pantanal of Mato Grosso, Brazil

    Directory of Open Access Journals (Sweden)

    Leandro D. Battirola

    2009-09-01

    Full Text Available In this study different sampling techniques for Diplopoda in soil, tree trunks and canopies were applied in an integrated way in the northern region of the Pantanal of Mato Grosso, Brazil. This was done in order to assess the relationship within the fauna in each forest strata, as well as its richness and temporal distribution. In all these habitats there were a total of 1,354 diplopods, distributed in four taxonomic orders, with Polyxenida being predominant over Polydesmida, Spirostreptida and Spirobolida. The largest representation was found on the trunks of the Vochysia divergens (721 ind., intercepted by tree photoecletors, whereas in the canopies sampling reached only 65 specimens. In the edaphic stratum 568 diplopods were captured, most with the use of the Winkler extractor, followed by pitfall traps and soil photoecletors. In spite of being an important group in these environments, both in terms of richness and diversity, this was less than has been observed in other Neotropical areas. However, due to seasonal changes in the Pantanal the existence of a relationship between the soil and the tree fauna was found as well as different survival strategies observed during the flood period. Regarding vertical distribution, the greatest richness and variety of taxonomic groups was found in the forest's edaphic environment demonstrating its association mainly with this forest stratum.

  12. Natural gas distribution in Brazil - opportunities of improvement; Distribuicao de gas natural no pais - oportunidades de melhoria

    Energy Technology Data Exchange (ETDEWEB)

    Correa, Silvia R. [PETROBRAS, Rio de Janeiro, RJ (Brazil); Quintella, Odair M.; Farias Filho, Jose R. de [Universidade Federal Fluminense, Niteroi, RJ (Brazil)

    2005-07-01

    Great are the challenges established by the Brazilian Government related to goals to be achieved for the increment of the Natural Gas participation in brazilian energetic matrix, from current 5% to 12%, up to 2010. The enlargement of the distribution infrastructure of the gas (gas-pipelines 'mesh') in Brazil is considered one of the greatest challenges for the growth of the Brazilian market of Natural Gas, accomplishment that involves elevated investments. This paper presents a model of Management System for the good organizational performance of the small Natural Gas Supplying Brazilian Companies focused on criteria of Leadership, Strategies and Plans and Results, established by the Premio TOP Empresarial and by the 'Rumo a Excelencia', held by the 'Progama Qualidade Rio' and 'Fundacao para o Premio Nacional da Qualidade', respectively. The management practices of these companies were reviewed, considering the context of the energetic Brazilian scenario, subjected to the political and operational definitions and uncertainties, the available financial resources, limited or not prioritized, and actual barriers to be surpassed by the Gas Supplying Companies in order to achieve the pre-established government goals for this segment. The implementation of the proposed simplified Model, seen as improvement opportunities for the segment of Natural Gas distribution, will lead the Gas Distribution Companies to a intermediary stage envisioning the real steps towards the excellence of the performance. (author)

  13. Phenotypic and genotypic profile of pyrethroid resistance in populations of the mosquito Aedes aegypti from Goiânia, Central West Brazil

    Directory of Open Access Journals (Sweden)

    Francesca Guaracyaba Garcia Chapadense

    2015-10-01

    Full Text Available ABSTRACTINTRODUCTION:The mosquito Aedes aegypti has evolved resistance to pyrethroid insecticides. The present study evaluated Ae. aegypti from Goiânia for the resistant phenotype and for mutations associated with resistance.METHODS:Insecticide dose-response bioassays were conducted on mosquitoes descended from field-collected eggs, and polymerase chain reaction (PCR was used to genotype 90 individuals at sites implicated in pyrethroid resistance.RESULTS:All mosquito populations displayed high levels of resistance to deltamethrin, as well as high frequencies of the 1016Ile kdr and 1534Cys kdrmutations.CONCLUSIONS:Aedes aegypti populations in the Goiânia area are highly resistant to deltamethrin, presumably due to high frequencies of kdr(knockdown-resistance mutations.

  14. Taxonomic key for the genera of Elmidae (Coleoptera, Byrrhoidea occurring in Goiás State, Brazil, including new records and distributional notes

    Directory of Open Access Journals (Sweden)

    Felipe F. Barbosa

    2013-06-01

    Full Text Available A taxonomic key for the genera of Elmidae (Coleoptera, Byrrhoidea occurring in Goiás State, Brazil, including new records and distributional notes. Despite their great diversity and high abundance in Neotropical aquatic environments, the fauna of Elmidae remains practically unknown in some areas and even entire biomes in this region. In this work we bring, for the first time, faunistic data for the Elmidae of central Brazil. The aim of this work was to inventory the Elmidae fauna in central, southwestern and southeastern Goiás State, Brazil and to produce a taxonomic key, at genus level, for adults from the studied region. The taxonomic key presented herein offers means for the identification of all the 13 genera known to occur in Goiás, 11 of them being new records for the State. Moreover, the number of named species registered for Goiás increased from one to nine.

  15. Spatial distribution of schistosomiasis foci on Itamaracá Island, Pernambuco, Brazil

    Directory of Open Access Journals (Sweden)

    CS Barbosa

    2004-08-01

    Full Text Available Acute cases of schistosomiasis have been found on the coastal area of Pernambuco, Brazil, due to environmental disturbances and disorderly occupation of the urban areas. This study identifies and spatially marks the main foci of the snail host species, Biomphalaria glabrata on Itamaracá Island. The chaotic occupation of the beach resorts has favoured the emergence of transmission foci, thus exposing residents and tourists to the risk of infection. A database covering five years of epidemiological investigation on snails infected by Schistosoma mansoni in the island was produced with information from the geographic positioning of the foci, number of snails collected, number of snails tested positive, and their infection rate. The spatial position of the foci were recorded through the Global Positioning System (GPS, and the geographical coordinates were imported by AutoCad. The software packages ArcView and Spring were used for data processing and spatial analysis. AutoCad 2000 was used to plot the pairs of coordinates obtained from GPS. Between 1998 and 2002 5009 snails, of which 12.2% were positive for S. mansoni, were collected in Forte Beach. A total of 27 foci and areas of environmental risk were identified and spatially analyzed allowing the identification of the areas exposed to varying degrees of risk.

  16. Chaetognatha of the Brazil-Malvinas (Falkland confluence: distribution and associations

    Directory of Open Access Journals (Sweden)

    Alina M. Crelier

    Full Text Available The planktonic chaetognaths from the Brazil-Malvinas (Falkland confluence, extending between 36º 30' - 50º 5' S and 60º 33' - 41º 7' W, were studied. Ten species were found: Eukrohnia hamata (Möbius, 1875 (Eukrohniidae, Pterosagitta draco (Krohn, 1853 (Pterosagittidae, Sagitta enflata Grassi, 1881, Sagitta gazellae Ritter-Zahony, 1909, Sagitta hexaptera d´Orbigny, 1834, Sagitta lyra Krohn, 1853, Sagitta minima Grassi, 1881, Sagitta planctonis Steinhaus, 1896, Sagitta serratodentata Krohn, 1853, and Sagitta tasmanica Thomson, 1947 (Sagittidae. Sagitta gazellae was the most abundant species followed by E. hamata, S. tasmanica and S. serratodentata. The association analysis among the different species, salinity and temperature revealed two groups of species, one related to higher salinities and warmer waters (P. draco, S. hexaptera and S. serratodentata and the other to lower salinities and colder waters (E. hamata, S. gazellae and S. tasmanica. The fact that P. draco and S. hexaptera, formerly defined as warm-water species, appeared further south than previously reported might be related to the existence of warm core eddies up to 46º S in September and October 1988.

  17. Distribution of the type III DNA methyltransferases modA, modB and modD among Neisseria meningitidis genotypes: implications for gene regulation and virulence.

    Science.gov (United States)

    Tan, Aimee; Hill, Dorothea M C; Harrison, Odile B; Srikhanta, Yogitha N; Jennings, Michael P; Maiden, Martin C J; Seib, Kate L

    2016-01-01

    Neisseria meningitidis is a human-specific bacterium that varies in invasive potential. All meningococci are carried in the nasopharynx, and most genotypes are very infrequently associated with invasive meningococcal disease; however, those belonging to the 'hyperinvasive lineages' are more frequently associated with sepsis or meningitis. Genome content is highly conserved between carriage and disease isolates, and differential gene expression has been proposed as a major determinant of the hyperinvasive phenotype. Three phase variable DNA methyltransferases (ModA, ModB and ModD), which mediate epigenetic regulation of distinct phase variable regulons (phasevarions), have been identified in N. meningitidis. Each mod gene has distinct alleles, defined by their Mod DNA recognition domain, and these target and methylate different DNA sequences, thereby regulating distinct gene sets. Here 211 meningococcal carriage and >1,400 disease isolates were surveyed for the distribution of meningococcal mod alleles. While modA11-12 and modB1-2 were found in most isolates, rarer alleles (e.g., modA15, modB4, modD1-6) were specific to particular genotypes as defined by clonal complex. This suggests that phase variable Mod proteins may be associated with distinct phenotypes and hence invasive potential of N. meningitidis strains. PMID:26867950

  18. Distribution of the type III DNA methyltransferases modA, modB and modD among Neisseria meningitidis genotypes: implications for gene regulation and virulence.

    Science.gov (United States)

    Tan, Aimee; Hill, Dorothea M C; Harrison, Odile B; Srikhanta, Yogitha N; Jennings, Michael P; Maiden, Martin C J; Seib, Kate L

    2016-01-01

    Neisseria meningitidis is a human-specific bacterium that varies in invasive potential. All meningococci are carried in the nasopharynx, and most genotypes are very infrequently associated with invasive meningococcal disease; however, those belonging to the 'hyperinvasive lineages' are more frequently associated with sepsis or meningitis. Genome content is highly conserved between carriage and disease isolates, and differential gene expression has been proposed as a major determinant of the hyperinvasive phenotype. Three phase variable DNA methyltransferases (ModA, ModB and ModD), which mediate epigenetic regulation of distinct phase variable regulons (phasevarions), have been identified in N. meningitidis. Each mod gene has distinct alleles, defined by their Mod DNA recognition domain, and these target and methylate different DNA sequences, thereby regulating distinct gene sets. Here 211 meningococcal carriage and >1,400 disease isolates were surveyed for the distribution of meningococcal mod alleles. While modA11-12 and modB1-2 were found in most isolates, rarer alleles (e.g., modA15, modB4, modD1-6) were specific to particular genotypes as defined by clonal complex. This suggests that phase variable Mod proteins may be associated with distinct phenotypes and hence invasive potential of N. meningitidis strains.

  19. [DISTRIBUTION OF GENOTYPES OF C825T POLYMORPHISM β3-SUBUNIT G-PROTEIN GENE IN PATIENTS WITH ARTERIAL HYPERTENSION ACCORDING THE DEGREE OF OBESITY].

    Science.gov (United States)

    Moiseyenko, I; Prystupa, L; Garbuzova, V; Pogorielova, O; Opolonskaya, N

    2015-01-01

    Arterial hypertension (AH) and obesity - risk factors for cardiovascular diseases and their complications, leading to high morbidity and mortality. These nosologies notedly linked, because have common etiological factors, pathophysiological mechanisms and genetic determination. The aim this research was to analyze the distribution of genotypes of the C825T polymorphism of β3-subunit G-protein gene (GNB3) according the degree of obesity and to assess the risk of obesity in patients with AH. Patients were divided into three groups according the degree of obesity. We used clinical, anthropometric, instrumental, molecular-genetic and statistical methods. The significance of differences of alleles and genotypes frequency was determined by test χ². For comparing the groups used nonparametric Mann-Whitney and Kruskal-Wallis tests. A value of phypertension and obesity (χ² = 27,976, p obesity. The risk of obesity in T allele carriers was in 2.2 times higher than in C allele carriers in patients with AH. In summary, our study showed association of C825T polymorphism of the GNB3 with obesity, but did not prove the association this with the degree of obesity i patients with AH.

  20. Prevalence, Genotype Distribution and Risk Factors for Cervical Human Papillomavirus Infection in the Grand Tunis Region, Tunisia.

    Directory of Open Access Journals (Sweden)

    Monia Ardhaoui

    Full Text Available Implementation of Human Papillomavirus (HPV vaccination should be considered a key cervical cancer prevention strategy in Tunisia, where Pap smear screening is not efficient. This study aims to estimate the prevalence and to identify risk factors associated with HPV infection among women from Grand Tunis, Tunisia. We conducted a cross-sectional study, between December 2012 and May 2013. Eligible women for this study were those aged 18-65 years, sexually active, who sought medical attention at their primary health care centre or clinic in Grand Tunis, Tunisia and who gave written consent. A liquid-based Pap smear sample was obtained from all women using a cervical brush. Only women with betaglobin positive test were further analysed for HPV detection and typing. A nested-PCR of the L1 region was performed followed by reverse line blot hybridization to facilitate the specific detection of 31 HPV genotypes. Multiple logistic regression modeling was used for the analysis of associations between variables with some considered possible confounders after checking for interactions. A total of 391 women were enrolled in this study and 325 out of the 391 cervical samples were positive for the betaglobin test. Overall HPV prevalence was 13.2% [9.8%-17.5%], with the following most prevalent HPV genotypes: HPV6 (40%, HPV40 (14%, HPV16 (12%, HPV52 (9%, HPV31 and HPV59 (7%, followed by HPV68 (4%. Mean age of HPV positive women was 40.7±0.92 years. Independently associated risk factors of HPV infection were smoking (OR:2.8 [0.8-9.6], low income (OR:9.6 [1.4-63.4, bad housing type (OR:2.5 [1-6.8], partner with multiple sexual relationship (OR:4.5 [0.9-22.9] and single women (widowed, divorced, separated, never married (OR:6.9 [1.1-42.2]. This study provides the first national-based estimate of HPV prevalence in Tunisia. Our findings contribute to the evidence on the current burden of HPV infection, the critical role of sexual behaviour and socioeconomic status

  1. IMPLEMENTATION OF DISTRIBUTION CENTERS AS LOGISTICS COMPETITIVE ADVANTAGE: STUDY ON OIL COMPANY DISTRIBUTOR IN SOUTHEAST BRAZIL

    Directory of Open Access Journals (Sweden)

    Henrique Albernaz

    2014-12-01

    Full Text Available This study aims to present how distribution centers implementation in organization is able to provide a competitive advantage over its competitors. The qualitative research was based on multiple case studies. Thus, these cases were focused on lubricants segment national distribution company. It was intended to introduce improvements recognizing distribution centers (DCs importance as competitive advantage. DC Macaé-RJ and Piracicaba-SP were chosen to represent this scenario. Therefore, as results it was found increased sales and operating leverage of results within the market in which it operates.

  2. Impact of genotype and cooking style on the content, retention, and bioacessibility of β-carotene in biofortified cassava (Manihot esculenta Crantz) conventionally bred in Brazil.

    Science.gov (United States)

    Berni, Paulo; Chitchumroonchokchai, Chureeporn; Canniatti-Brazaca, Solange G; De Moura, Fabiana F; Failla, Mark L

    2014-07-16

    Biofortification is a strategy for decreasing micronutrient deficiencies in vulnerable populations by increasing nutrient density in staple food crops. Roots from five varieties of cassava biofortified with β-carotene (βC), three parental accessions, and one variety of commonly consumed white cassava from Brazil were investigated. Roots from biofortified varieties contained up to 23-fold higher βC than white cassava, and the additional complement of βC was primarily the all-trans isomer. At least 68% of βC per gram fresh weight was retained after boiling or boiling and briefly frying. Micellarization of βC during simulated digestion of fried root exceeded that of boiled root. Apical uptake of all-trans-βC from mixed micelles by Caco-2 cells was affected by an interaction between variety and cooking style. These results suggest that Brazilian cassava biofortified with βC has the potential to reduce vitamin A deficiency without requiring major changes in local and ethnic styles of home cooking.

  3. Factors structuring the spatial distribution of Chironomidae larvae community in the floodplain of the northern Pantanal, Brazil

    Directory of Open Access Journals (Sweden)

    Maitê Tambelini dos Santos

    2013-06-01

    Full Text Available AIM: In order to identify the factors structuring the spatial distribution of the Chironomidae community, we analyzed the effects of water and sediment characteristics, flood duration and type of phytophysiognomic unit on the taxa composition of this community. MÉTODOS: O estudo foi realizado em 26 parcelas do Sítio de Amostragem de Longa Duração (SALD, localizado em uma área sazonalmente inundável na parte norte do Pantanal (Brasil. The study was conducted at 26 plots of the Long-Term Sampling Site, located in a seasonally flooded area in northern Pantanal (Brazil. Sediment samples were taken from each plot to analyze the Chironomidae community, organic matter and particle size; limnological parameters (depth, water temperature, dissolved oxygen, electrical conductivity and pH; besides the duration of flood. RESULTS: We identified 432 individuals distributed into 21 Chironomidae taxa. Polypedilum and Ablabesmyia were the most frequent spatially. The multivariate multiple regression analysis showed that the community composition was significantly related to the flood duration and grain size characteristics, but not to phytophysiognomic units and limnological parameters. Plots with predominantly sandy substrate and flooded for a shorter time presented a greater occurrence of taxa. CONCLUSIONS: The gradient produced by the spatial distribution of sediment grain size and flood duration determined the distribution of Chironomidae taxa. Changes in the hydrological functioning of the Pantanal, such as those from reservoir construction in the uplands region and roads and dykes construction in the floodplain could then impact the local chironomid biodiversity.

  4. Distribution and conservation of three important bird groups of the Atlantic Forest in north-east Brazil.

    Science.gov (United States)

    Pereira, G A; Araújo, H F P; Azevedo-Júnior, S M

    2016-06-27

    The Pernambuco Endemism Center in north-east Brazil has the most fragmented forest cover and the largest number of threatened birds of the whole Atlantic Forest. We analyzed the distribution of three groups of bird species: forest-dependent, endemic and/or threatened using the interpolation method of Inverse Distance Weighting. We also checked the concentration of these birds in protected and unprotected areas, suggesting new sites that need to be protected. The richness concentration of forest-dependent, endemic and/or threatened birds in 123 sites were analysed. There was a greater concentration of the three groups in north Alagoas, south and north Pernambuco, and north and west Paraíba. The distribution of the three groups was almost regular in different vegetation types, although a lower concentration was found in the pioneer formation. There was a greater concentration of birds from all three groups between Pernambuco and Alagoas, and this must be due to the presence of more forest fragments with better structure and vegetation heterogeneity. The protected and unprotected areas hosted important records of endemic and/or threatened birds. We suggested some important places for implementation of new protected areas due to the larger concentrations of the target birds and because they are located within the boundaries of the Important Bird Areas. PMID:27355983

  5. Abundance and spatial-temporal distribution of the shrimp Xiphopenaeus kroyeri (Decapoda: Penaeidae): an exploited species in southeast Brazil.

    Science.gov (United States)

    Silva, E R; Sancinetti, G S; Fransozo, A; Azevedo, A; Costa, R C

    2016-04-19

    This study evaluated the abundance and spatial-temporal distribution of the shrimp Xiphopenaeus kroyeri in the coastal region of Macaé, state of Rio de Janeiro, southeastern Brazil. Monthly samples were obtained from March 2008 to February 2010 in six stations located in Inner (5, 10 and 15m depth) and Outer (25, 35 and 45m depth) areas. It was used a commercial fishery boat equipped with an otter-trawl net (3.5 m mouth width, mesh size 20mm and 15mm in the cod end). Water samples were taken for determination of temperature and salinity, and sediment samples for determination of texture and organic matter content. A total of 7146 shrimps were sampled. About 95% of all shrimps were caught in the shallow area, i.e., depths <20m. Greatest abundances were recorded in winter and spring. No significant correlation was observed between sediment (phi) and abundance. The distribution of X. kroyeri in the studied area was closely related to seasonal cold waterfront of the South Atlantic Central Water (SACW) and temperature was the main factor affecting the species abundance.

  6. Influence of environmental variables on the distribution of intertidal porcellanid crabs in Sepetiba Bay, Rio de Janeiro, Brazil

    Directory of Open Access Journals (Sweden)

    Luciana A de Mattos

    2014-11-01

    Full Text Available This study aims to assess the distribution patterns of porcellanid crabs in the intertidal zone of Sepetiba Bay, Rio de Janeiro, Brazil, identifying the abiotic determinants. Samples were collected seasonally (dry and rainy seasons from January 2005 to August 2007, during low tides in external, intermediate and internal sectors. The crabs were hand caught in consolidated substrates with sampling effort of two people in three different flood zones (lower, medium and higher for 30 min. Water temperature, salinity, accumulated rainfall, the physical and chemical variables of sediment were analyzed concerning interaction in the distribution patterns of the species. A total of 3,389 crabs, belonging to five genera and eight species, were obtained. With the exception of Petrolisthes armatus, found throughout the intertidal range, all species inhabited the intertidal bottom of Sepetiba Bay exclusively. Favorable environmental conditions of the Sepetiba Bay enabled the success of colonization of several porcellanid species which are a refuge and feeding environment of the Marambaia Island and continental (Junqueira and Ibicuí of the outer zone. The number of species recorded in this study supporting the hypothesis that features of substratum and the availability of food and refuges are factors limiting the establishment of this family in Sepetiba Bay.

  7. Filling data gaps on the diversity and distribution of Amazonian bats (Chiroptera: the case of Amapá, easternmost Brazil

    Directory of Open Access Journals (Sweden)

    Ana C. M. Martins

    2011-04-01

    Full Text Available Brazil is the second most bat species-rich country in the world, but the information on its species diversity, occurrence and distribution is still heterogeneous and fragmented. None of the Brazilian biomes are well surveyed for bats, but this situation is more critical in Amazonia, an area covering nearly 2/3 of the country. Here we provide updated information on the bats of Amapá, once a data gap in the diversity and distribution of bats in the easternmost Amazonia, and the Guiana Shield as well. Rapid biological assessments (5,551 mistnet.hours were conducted in conservation units and areas of concern, resulting in 1,695 captures, 59 species, 36 genera and six families for the State. New records for the state and for the Guiana Shield area are reported. With our records, 82 species of bats are currently known in Amapá, filling a gap in the knowledge of bat fauna in the Amazon River's delta region.

  8. Spatial distribution of the estuarine ichthyofauna of the Rio Formoso (Pernambuco, Brazil, with emphasis on reef fish

    Directory of Open Access Journals (Sweden)

    Andréa C. G. de Paiva

    2009-06-01

    Full Text Available The objectives of the present study were to identify species of reef fish that use the Rio Formoso estuary (northeastern Brazil as a refuge and natural nursery and to describe the spatial distribution of the estuary fish fauna in rainy and dry seasons. A total of 5475 specimens, across 78 species and 39 families, were analyzed; 51.3% of the species were of reef origin. Among these, Eucinostomus melanopterus (Bleeker, 1863, E. gula (Cuvier, 1830, and Sphoeroides testudineus (Linnaeus, 1758, in this order, were the most abundant in the upper estuary; S. greeleyi Gilbert, 1900, E. melanopterus, and Lutjanus synagris (Linnaeus, 1758 were the most abundant in the mid estuary; and E. gula and Albula vulpes (Linnaeus, 1758 in the lower estuary. The percentages of reef species were 39.2%, 54.2% and 66.7% for the upper, mid and lower regions, respectively. The greatest diversity was found in the upper estuary and greatest abundance occurred in the mid region. The type of sediment was a strong determinant of the spatial distribution of fish fauna. The most abundant species were found in a mesohaline (5-18 to euhaline (30-40 salinity regimen, suggesting some capacity for osmotic regulation. The fish fauna of the Rio Formoso estuary receives a direct influence from the reefs and coastal region between Sirinhaém and Tamandaré, thereby providing a greater richness of reef fish.

  9. Updating the geographical distribution and frequency of Aedes albopictus in Brazil with remarks regarding its range in the Americas

    Directory of Open Access Journals (Sweden)

    Roberta Gomes Carvalho/

    2014-09-01

    Full Text Available The geographical distribution of Aedes albopictus in Brazil was updated according to the data recorded across the country over the last eight years. Countrywide house indexes (HI for Ae. albopictus in urban and suburban areas were described for the first time using a sample of Brazilian municipalities. This mosquito is currently present in at least 59% of the Brazilian municipalities and in 24 of the 27 federal units (i.e., 26 states and the Federal District. In 34 Brazilian municipalities, the HI values for Ae. albopictus were higher than those recorded for Ae. aegypti, reaching figures as high as HI = 7.72 in the Southeast Region. Remarks regarding the current range of this mosquito species in the Americas are also presented. Nineteen American countries are currently infested and few mainland American countries have not confirmed the occurrence of Ae. albopictus. The large distribution and high frequency of Ae. albopictus in the Americas may become a critical factor in the spread of arboviruses like chikungunya in the new world.

  10. Abundance and spatial-temporal distribution of the shrimp Xiphopenaeus kroyeri (Decapoda: Penaeidae): an exploited species in southeast Brazil.

    Science.gov (United States)

    Silva, E R; Sancinetti, G S; Fransozo, A; Azevedo, A; Costa, R C

    2016-04-19

    This study evaluated the abundance and spatial-temporal distribution of the shrimp Xiphopenaeus kroyeri in the coastal region of Macaé, state of Rio de Janeiro, southeastern Brazil. Monthly samples were obtained from March 2008 to February 2010 in six stations located in Inner (5, 10 and 15m depth) and Outer (25, 35 and 45m depth) areas. It was used a commercial fishery boat equipped with an otter-trawl net (3.5 m mouth width, mesh size 20mm and 15mm in the cod end). Water samples were taken for determination of temperature and salinity, and sediment samples for determination of texture and organic matter content. A total of 7146 shrimps were sampled. About 95% of all shrimps were caught in the shallow area, i.e., depths <20m. Greatest abundances were recorded in winter and spring. No significant correlation was observed between sediment (phi) and abundance. The distribution of X. kroyeri in the studied area was closely related to seasonal cold waterfront of the South Atlantic Central Water (SACW) and temperature was the main factor affecting the species abundance. PMID:27097079

  11. Diversity and distribution of the bryophyte flora in montane forests in the Chapada Diamantina region of Brazil

    Directory of Open Access Journals (Sweden)

    Emilia de Brito Valente

    2013-09-01

    Full Text Available Bryophytes constitute an important component of tropical rain forests, which provide microhabitats favorable for their establishment. Bryophytes are also quite responsive to changes in microclimate, which makes them good bioindicators. This study aimed to determine the diversity and distribution of bryophytes in upper and lower montane forests of the Chapada Diamantina region of the state of Bahia, Brazil. To that end, we studied community aspects such as richness, diversity, substrates colonized, life forms and floristic similarity between areas and regions. In 2007 and 2008, we collected specimens from six forest sites, located from the north to the south of the Chapada Diamantina region. We identified a total of 205 infrageneric taxa. In comparison with the lower montane forests, the upper montane forests presented higher diversity and species richness, as well as greater numbers of substrates colonized, life form types, species of restricted geographic distribution and species typical of shaded areas. We also found low similarity in the species composition, the populations of the upper and lower montane forests forming two large and distinct groups. Although presenting relatively high floristic homogeneity among themselves, the Chapada Diamantina areas presented little similarity with those of the Atlantic Forest. This can be explained by the differences between the two regions in terms of environmental conditions, precipitation, seasonality, elevation and continentality.

  12. Forest Fires in Southwestern Amazonia During 2005: Extent and Distribution in Eastern Acre State, Brazil

    Science.gov (United States)

    Brown, I.; Moulard, E. M.; Nakamura, J.; Schroeder, W.; Maldonado, M.; Vasconcelos, S. S.; Selhorst, D.

    2007-05-01

    The extended drought in western Amazonia during 2005 provided the conditions for wild fires that spread in old- growth rain forests and cleared areas of the contiguous areas of Madre de Dios, Peru, Acre, Brazil, and Pando, Bolivia, collectively known as the MAP Region. The greatest extent of the wild fires occurred in eastern Acre State with 60,000 km2 of diverse land uses that range from intensely occupied colonization areas, large cattle ranches, extractive and biological reserves and indigenous areas. At the request of the Public Ministry of Acre and other government agencies we analyzed Landsat 5 and CBERS 2 imagery for forests with canopies affected by fires, using visual interpretation and manual digitalization of polygons. Accuracy assessment was done with 180 aerial photos. The total area of forest with canopies affected by fires was 267,000 ha, roughly five times recent annual deforestation rates for Acre State. Omission and commission errors were 28% and 2%, respectively. Burn scars in non-forest areas were determined using ASTER and CBERS 2 imagery via supervised classification. Total open area with burn scars was 203,000 ha. The total of open area and forests affected by fires exceeded 470,000 ha due to three factors: (1) some images used did not include the last weeks of burning; (2) ground fires in forests that did not affect the canopy and therefore were not detected; and (3) concern of the interpreters to avoid commission errors. Of the twelve municipalities of eastern Acre, most affected were Acrelandia, Placido de Castro, Epitaciolandia with >31%, >19% and >17% of the municipality affected, respectively). The largest impact, >114,000 ha, occurred in the Rio Branco Municipality. Similar patterns of burning occurred in Pando and in Madre de Dios. The environmental, social and economic disaster that these fires produced may be a harbinger of future impacts in southwestern Amazonia if current climate predictions prove to be correct.

  13. Distribution of HPV Genotype in Invasive Cervical Carcinoma and Cervical Intraepithelial Neoplasia in Zhejiang Province, Southeast China: Establishing the Baseline for Surveillance

    Directory of Open Access Journals (Sweden)

    Xiao-Xian Xu

    2015-09-01

    Full Text Available Human papillomavirus (HPV are firmly established as the principal causative agent for cervical carcinoma. Current vaccines may provide some protection for women from cervical carcinoma linked to HPV genotype 16 and 18. This may be the best vaccine for Western women, but the geographical variation in HPV distributions may not make it the most appropriate vaccine for China or Asia. This study provided an observational, retrospective, hospital-based cross-sectional study on the distribution of HPV genotypes among 5410 women with invasive cervical cancer (ICC or cervical intraepithelial neoplasia (CIN. Overall, the positive rates of the four HPV types included in current prophylactic vaccines were counted, the two high-risk types (HPV-16 and -18 covered by current vaccines represented 66.9% of women with squamous cancer, 55.0% with adenocarcinoma, 64.9% with adenosquamous carcinoma and 77.4% of other type ICC, as well as 59.5% of CIN III, 45.0% of CIN II and 38.1% of CIN I cases. As expected, two low-risk types (HPV-6 and -11 included in the quadrivalent vaccine did not show good coverage data. Particularly worth mentioning is the fact that the addition of HPV-52 and -58 to the vaccine cocktail would increase cancer protection in our population, potentially preventing up to beyond 16% of squamous/adenosquamous carcinoma and other type of cervical cancers, and 7.75% of adenocarcinomas. It might also potentially reduce the rate of CIN III by a further 28.6% and CIN II and I by a third. This study established the baseline for surveillance in Zhejiang Province, and provides data for further vaccine designs: a quadrivalent HPV vaccine covering HPV-16/-58/-18/-52, would be more welcome in our region in the forthcoming year compared to the currently available vaccine.

  14. Firm size and productivity. Evidence from the electricity distribution industry in Brazil

    Energy Technology Data Exchange (ETDEWEB)

    Tovar, Beatriz [Departmento de Analisis Economico Aplicado y EIT, Universidad de Las Palmas de Gran Canaria (Spain); Javier Ramos-Real, Francisco [Departamento de Analisis Economico, Facultad de Ciencias Economicas y Empresariales, Campus de Guajara, Universidad de La Laguna, La Laguna, S/C de Tenerife, Espana (Spain); De Almeida, Edmar Fagundes [IE-UFRJ (Instituto de Economia-Universidade Federal do Rio de Janeiro) (Brazil)

    2011-02-15

    In this paper we apply Stochastic Frontier Analysis through a distance function to investigate the impact of firm size on productivity development in electricity distribution. We use a sample of seventeen Brazilian firms from 1998 to 2005 and decompose productivity into technical efficiency, scale efficiency and technical change. Moreover, a further step is to decompose the technical change measurement into several components. The results indicate that firm size is important for industry's productivity, and therefore a key aspect to consider when making decisions that affect the market structure in the electricity distribution industry. (author)

  15. Firm size and productivity. Evidence from the electricity distribution industry in Brazil

    International Nuclear Information System (INIS)

    In this paper we apply Stochastic Frontier Analysis through a distance function to investigate the impact of firm size on productivity development in electricity distribution. We use a sample of seventeen Brazilian firms from 1998 to 2005 and decompose productivity into technical efficiency, scale efficiency and technical change. Moreover, a further step is to decompose the technical change measurement into several components. The results indicate that firm size is important for industry's productivity, and therefore a key aspect to consider when making decisions that affect the market structure in the electricity distribution industry. (author)

  16. Genotype-environment interaction and the number of test sites for the genetic improvement of rubber trees (Hevea in São Paulo State, Brazil

    Directory of Open Access Journals (Sweden)

    Reginaldo Brito da Costa

    2000-03-01

    Full Text Available The present study quantifies the possible genotype-environment interactions and determines the ideal number of test sites for rubber trees [Hevea brasiliensis (Willd ex Adr. de Juss. Muell Arg] in the plateau region of São Paulo State. The study was based on the genetic correlation among progenies at three different sites and on estimates of genetic gains with indirect selection of rubber trees. Twenty-two half-sib progenies were planted at the Jaú, Pindorama and Votuporanga experimental stations using random blocks with five replications and 10 plants per plot. At three years of age, the plants were evaluated for their total number of latex ring vessels (NR, rubber production (RP, bark thickness (BT and girth (SG. There was significant genetic variability in the characters RP, SG and BT, mainly among progenies from Pindorama and Votuporanga. The effects of genotype-site interactions were significant for RP and SG. The finding of significant interactions was not a complicating factor because of the large genetic correlation detected. These results indicate that the use of two sites is more profitable when the gains in efficiency of selection are greater than 10%. Thus, Pindorama and Votuporanga will satisfactorily attend the studied region.O presente estudo objetivou quantificar possíveis interações de progênie x local, bem como determinar o número ideal de locais de experimentação com base em estimativas de correlação genética entre progênies nos diferentes locais e estimativa de ganhos genéticos com seleção indireta em seringueira [Hevea brasiliensis (Willd ex Adr. de Juss. Müell. Arg.]. Vinte e duas progênies de meio-irmãos foram plantadas nas Estações Experimentais de Jaú, Pindorama e Votuporanga, todas em SP, no delineamento de blocos ao acaso, com cinco repetições e dez plantas por parcela. Aos três anos de idade foram avaliados os caracteres: número total de anéis de vasos laticíferos, produção de borracha

  17. Breeding Guild Determines Frog Distributions in Response to Edge Effects and Habitat Conversion in the Brazil's Atlantic Forest.

    Science.gov (United States)

    Ferreira, Rodrigo B; Beard, Karen H; Crump, Martha L

    2016-01-01

    Understanding the response of species with differing life-history traits to habitat edges and habitat conversion helps predict their likelihood of persistence across changing landscape. In Brazil's Atlantic Forest, we evaluated frog richness and abundance by breeding guild at four distances from the edge of a reserve: i) 200 m inside the forest, ii) 50 m inside the forest, iii) at the forest edge, and iv) 50 m inside three different converted habitats (coffee plantation, non-native Eucalyptus plantation, and abandoned pastures, hereafter matrix types). By sampling a dry and a wet season, we recorded 622 individual frogs representing 29 species, of which three were undescribed. Breeding guild (i.e. bromeliad, leaf-litter, and water-body breeders) was the most important variable explaining frog distributions in relation to edge effects and matrix types. Leaf-litter and bromeliad breeders decreased in richness and abundance from the forest interior toward the matrix habitats. Water-body breeders increased in richness toward the matrix and remained relatively stable in abundance across distances. Number of large trees (i.e. DBH > 15 cm) and bromeliads best explained frog richness and abundance across distances. Twenty species found in the interior of the forest were not found in any matrix habitat. Richness and abundance across breeding guilds were higher in the rainy season but frog distributions were similar across the four distances in the two seasons. Across matrix types, leaf-litter species primarily used Eucalyptus plantations, whereas water-body species primarily used coffee plantations. Bromeliad breeders were not found inside any matrix habitat. Our study highlights the importance of primary forest for bromeliad and leaf-litter breeders. We propose that water-body breeders use edge and matrix habitats to reach breeding habitats along the valleys. Including life-history characteristics, such as breeding guild, can improve predictions of frog distributions in

  18. Breeding Guild Determines Frog Distributions in Response to Edge Effects and Habitat Conversion in the Brazil's Atlantic Forest.

    Directory of Open Access Journals (Sweden)

    Rodrigo B Ferreira

    Full Text Available Understanding the response of species with differing life-history traits to habitat edges and habitat conversion helps predict their likelihood of persistence across changing landscape. In Brazil's Atlantic Forest, we evaluated frog richness and abundance by breeding guild at four distances from the edge of a reserve: i 200 m inside the forest, ii 50 m inside the forest, iii at the forest edge, and iv 50 m inside three different converted habitats (coffee plantation, non-native Eucalyptus plantation, and abandoned pastures, hereafter matrix types. By sampling a dry and a wet season, we recorded 622 individual frogs representing 29 species, of which three were undescribed. Breeding guild (i.e. bromeliad, leaf-litter, and water-body breeders was the most important variable explaining frog distributions in relation to edge effects and matrix types. Leaf-litter and bromeliad breeders decreased in richness and abundance from the forest interior toward the matrix habitats. Water-body breeders increased in richness toward the matrix and remained relatively stable in abundance across distances. Number of large trees (i.e. DBH > 15 cm and bromeliads best explained frog richness and abundance across distances. Twenty species found in the interior of the forest were not found in any matrix habitat. Richness and abundance across breeding guilds were higher in the rainy season but frog distributions were similar across the four distances in the two seasons. Across matrix types, leaf-litter species primarily used Eucalyptus plantations, whereas water-body species primarily used coffee plantations. Bromeliad breeders were not found inside any matrix habitat. Our study highlights the importance of primary forest for bromeliad and leaf-litter breeders. We propose that water-body breeders use edge and matrix habitats to reach breeding habitats along the valleys. Including life-history characteristics, such as breeding guild, can improve predictions of frog

  19. The Design and Program Evaluation of a Distributed PBL Curriculum for Training Family Doctors in Brazil

    Science.gov (United States)

    Tomaz, Jose B. C.; van der Molen, Henk T.; Mamede, Silvia

    2013-01-01

    Over the past decade Problem-based Learning (PBL) and distance education have been combined as educational approaches in higher education. This combination has been called distributed PBL (dPBL). However, more research is needed to obtain more evidence and deeper insight in how to design and implement dPBL. The present study aims at describing the…

  20. Detection and distribution of rotavirus in raw sewage and creeks in Sao Paulo, Brazil

    Energy Technology Data Exchange (ETDEWEB)

    Mehnert, D.U.; Stewien, K.E. (Univ. of Sao Paulo (Brazil))

    1993-01-01

    Rotavirus invection is an important cause of hospitalization and mortality of infants and children in developing countries, especially where the water supply and sewage disposal systems are in precarious conditions. This report describes the detection, quantitation, and distribution of rotaviruses in domestic sewage and sewage polluted creeks in the city of San Paulo. 22 refs., 1 fig., 3 tabs.

  1. Vertical distribution of 137Cs in the native forest soil at Londrina region (Parana, Brazil)

    International Nuclear Information System (INIS)

    The 137 Cs depth distribution has been studied in several areas in the world and its knowledge is very important to verify its behavior in the soil matrix. The form of 137 Cs depth distribution more observed in an undisturbed soil it is exponential type. In this work, the study of 137 Cs depth distribution was accomplished at three native forests and a coffee yard, built before the 137 Cs fallout, in the region of Londrina (Parana). The sampling was conducted in 1 cm, 2 cm, 4 cm and 5 cm increment depth. The 137 Cs inventory observed for the forests was 248 Bq m-2 (Mata1), 338 Bq m-2 (Mata2) and 325 Bq m-2 (MataUEL). No 137 Cs activity was detected in the coffee yard. The 137 Cs depth distribution for the three forests presented the exponential type. For the soil class of the forests, 137 Cs adsorbed on the soil particles can be considered fixed or slowly exchangeable. (author)

  2. Firm size and productivity. Evidence from the electricity distribution industry in Brazil

    International Nuclear Information System (INIS)

    In this paper we apply Stochastic Frontier Analysis through a distance function to investigate the impact of firm size on productivity development in electricity distribution. We use a sample of seventeen Brazilian firms from 1998 to 2005 and decompose productivity into technical efficiency, scale efficiency and technical change. Moreover, a further step is to decompose the technical change measurement into several components. The results indicate that firm size is important for industry's productivity, and therefore a key aspect to consider when making decisions that affect the market structure in the electricity distribution industry. - Research Highlights: →We apply Stochastic Frontier Analysis through a distance function to investigate the impact of firm's size on productivity development in electricity distribution using a sample of eighteen Brazilian firms from 1998 to 2005. →Productivity is decomposed into technical efficiency, scale-efficiency and technical change. →Firm size is important for the industry's productivity, and therefore a key aspect to consider when making decisions that affect the market structure in the electricity distribution industry.

  3. Spatial distribution and diversity of bird community in an urban area of Southeast Brazil

    Directory of Open Access Journals (Sweden)

    Marco Antônio Manhães

    2005-03-01

    Full Text Available Since the campus of the Federal University of Juiz de Fora, Minas Gerais State, Brazil, has different landscapes, it turns up to be a useful ecological model to evaluate the influence of habitat heterogeneity on bird communities. Our research goals were to know the local avifauna and compare its composition and bird diversity within the different landscapes. Species were identified in point counts without distance estimation, in four habitats: secondary woodlot, lake and surroundings, scrub/abandoned grazing areas and urban areas. One hundred and twenty-one species were identified, but no difference in diversity among the habitats was found. However, analyses indicated the existence of greater similarities among the sampling points belonging to the same kind of habitat. Results suggests that small and isolated forest fragments in urban areas fail to sustain a greater diversity than the adjacent areas, even though the environment's heterogeneous aspect favours local bird richness.O Campus da Universidade Federal de Juiz de Fora possui diferentes paisagens e pode representar um modelo ecológico útil para avaliar a influência da heterogeneidade de hábitats sobre a diversidade de aves. Os objetivos deste trabalho foram conhecer a avifauna local e comparar a composição e diversidade de aves nas distintas paisagens existentes. As espécies foram identificadas em pontos de contagem, em quatro hábitats: mata secundária, lago e arredores, capoeira/pastagem abandonada e urbanizada/jardinada. Foram identificadas 121 espécies, mas não houve diferença de diversidade entre os hábitats. Entretanto, as análises indicaram a existência de maiores similaridades entre os pontos amostrais pertencentes ao mesmo tipo de hábitat. Os resultados sugerem que pequenos fragmentos de mata fortemente isolados em áreas urbanas não sustentam uma diversidade maior do que as áreas adjacentes mas o aspecto heterogêneo do ambiente pode favorecer a riqueza de aves

  4. The effectiveness of retreatment with peginterferon alfa and ribavirin in patients with chronic viral hepatitis C genotype 2 and 3: a prospective cohort study in Brazil

    Directory of Open Access Journals (Sweden)

    Artico Simara

    2012-12-01

    Full Text Available Abstract Background More than 50% of patients infected with chronic hepatitis C virus (HCV do not respond to treatment with conventional interferon (IFN combined with ribavirin (RBV. The aim of our study was to evaluate the effectiveness of retreatment with peginterferon alfa-2a or 2b (PEG-IFN 2a or 2b concomitantly with RBV in patients with HCV genotype 2 and 3, which were non-responders or relapsers to initial treatment with IFN / RBV and to identify possible predictors of sustained virological response (SVR. Methods From September 2003 to March 2009 a cohort of 216 patients who had previously failed therapy with a regimen of standard interferon and ribavirin, were followed in a specialized service implemented in the Brazilian Unified Health System, Rio Grande do Sul. All patients were retreated with PEG-IFN 2a or 2b per week, associated with RBV, through oral route, with doses determined according to weight (1,000 mg if weight ≤ 75 Kg and 1,250 mg if ≥ 75 Kg per day for 48 weeks. The HCV-RNA was tested by Polymerase Chain Reaction (PCR. Virological Response (VR within 48 weeks and SVR in the 72 weeks was considered for evaluation of treatment efficacy. Analyses were performed in patients who received at least one dose of PEG-IFN. Results The SVR rate for non-responders to previous treatment was 34.4% and for relapsers was 50% (p = 0.031. As predictive factors that contribute to improve SVR, were identified the age (p = 0.005, to be relapsers to previous treatment (p = 0.023 and present liver biopsy examination Metavir F0-F2 (p = 0.004. In assessing the safety profile, 51 patients (23.6% discontinued treatment prematurely. Conclusions This alternative retreatment for patients who have failed prior therapies for anti-HCV, has demonstrated promising SVR rate, provided that it includes a careful selection of patients with predictors of response and adverse events monitored.

  5. Distribution of superantigens in group A streptococcal isolates from Salvador, Brazil

    OpenAIRE

    Berman, Hillary F; Tartof, Sara Yee; Reis, Joice N; Reis, Mitermayer G.; Lee W Riley

    2014-01-01

    Background Group A streptococcus (GAS) causes invasive disease, superficial disease, and can asymptomatically colonize humans. Superantigens are one virulence factor found in GAS. Previous studies found associations between the genes that encode superantigens and emm type of GAS. It is unknown if these associations are due to underlying biological factors that limit the distribution of superantigens or, alternatively, if these associations are due to the expansion of local GAS linages where t...

  6. Regional validity of the parameters of a distributed runoff-erosion model in the semi-arid region of Brazil

    Institute of Scientific and Technical Information of China (English)

    SRINIVASAN; Vajapeyam; Srirangachar; PAIVA; Fernanda; Maria; de; Lima

    2009-01-01

    The present paper examines the validity of the application of a distributed rainfall-runoff-erosion model on a regional basis in a semi-arid region of Brazil. The model tested is known as WESP (Water Erosion Simulation Program) developed by Lopes (1987). The model simulates the hydrograph and the sediment graph for individual events and thus, the model when properly calibrated can serve as a predictive tool for runoff and soil erosion on an individual or a continuous basis. The possibility of the existence of regionally applicable values for the parameters of the model WESP would be very interesting from the point of view of predictability of runoff and soil erosion from ungauged basins in the region. For this purpose, data collected in several experimental units in the experimental basin of Sumé as well as in the experimental basin of So Joo de Cariri have been used. The model was calibrated and validated in each of the experimental units (consisting of erosion plots and micro-basins) and the average value from all of the units for each of the parameters was considered to be the regional value. With these average values, all the events in all the experimental units were simulated. The results show that the runoff and erosion values simulated with this single parameter set were very good in all the units, being well within the acceptable deviations.

  7. Regional validity of the parameters of a distributed runoff-erosion model in the semi-arid region of Brazil

    Institute of Scientific and Technical Information of China (English)

    SRINIVASAN Vajapeyam Srirangachar; PAIVA Fernanda Maria de Lima

    2009-01-01

    The present paper examines the validity of the application of a distributed rainfall-runoff-erosion model on a regional basis in a semi-arid region of Brazil.The model tested is known as WESP (Water Erosion Simulation Program) developed by Lopes (1987).The model simulates the hydrograph and the sedi-ment graph for individual events and thus, the model when properly calibrated can serve as a predic-tive tool for runoff and soil erosion on an individual or a continuous basis.The possibility of the exis-tence of regionally applicable values for the parameters of the model WESP would be very interesting from the point of view of predictability of runoff and soil erosion from ungauged basins in the region.For this purpose, data collected in several experimental units in the experimental basin of Sume as well as in the experimental basin of Sao Joao de Cariri have been used.The model was calibrated and validated in each of the experimental units (consisting of erosion plots and micro-basins) and the av-erage value from all of the units for each of the parameters was considered to be the regional value.With these average values, all the events in all the experimental units were simulated.The results show that the runoff and erosion values simulated with this single parameter set were very good in all the units, being well within the acceptable deviations.

  8. Stratigraphical discontinuities, tropical landscape evolution and soil distribution relationships in a case study in SE-Brazil

    Directory of Open Access Journals (Sweden)

    M. Cooper

    2002-09-01

    Full Text Available On a regional summit surface in the county of Piracicaba (SP within the Peripheric Depression of São Paulo, formed of discontinued flattened tops, there is an abrupt transition between a Typic Hapludox and a Kandiudalfic Eutrudox, together with two stoneline layers. Using stratigraphical, mineralogical, and cartographic studies, this transition and the soil distribution of this surface were studied, correlating them with the different parent materials and the morphoclimatic model of landscape evolution in Southeastern Brazil. The Typic Hapludox was formed on a sandy Cenozoic deposit (Q that overlies a pellitic deposit of the Iratí formation (Pi, representing a regional erosive discordance. Westwards to the Piracicaba River, this sequence is interrupted by a diabase sill overlain by a red clayey material which gave origin to the Kandiudalfic Eutrudox. Two post-Permian depositional events were identified by the two stonelines and stratigraphical discontinuities. The first event generated the deposition of a sandy sediment in the form of levelled alveoluses on regional barriers, most of these formed by dikes and diabase sills, probably during a drier phase. The second depositional event, leading to the deposition of the red clay was probably the dissection of the previously formed pediplane during a humid climate, followed by another pedimentation process during a later, drier period.

  9. Spatial distribution and enteroparasite contamination in peridomiciliar soil and water in the Apucaraninha Indigenous Land, southern Brazil.

    Science.gov (United States)

    da Silva, Joseane Balan; Piva, Camila; Falavigna-Guilherme, Ana Lúcia; Rossoni, Diogo Francisco; de Ornelas Toledo, Max Jean

    2016-04-01

    The prevalence and distribution of soil and water samples contaminated with enteroparasites of humans and animals with zoonotic potential (EHAZP) in Apucaraninha Indigenous Land (AIL), southern Brazil, was evaluated. An environmental survey was conducted to evaluate the presence of parasitic forms in peridomiciliary soil and associated variables. Soil samples were collected from 40/293 domiciles (10 domiciles per season), from November 2010 to June 2011, and evaluated by modified methods of Faust et al. and Lutz. Analyses of water from seven consumption sites were also performed. The overall prevalence of soil samples contaminated by EHAZP was 23.8 %. The most prevalent parasitic forms were cyst of Entamoeba spp. and eggs of Ascaris spp. The highest prevalence of contaminated soil samples was observed in winter (31 %). The probability map obtained with geostatistical analyses showed an average of 47 % soil contamination at a distance of approximately 140 m. The parasitological analysis of water did not detect Giardia spp. or Cryptosporidium spp. and showed that all collection points were within the standards of the Brazilian law. However, the microbiological analysis showed the presence of Escherichia coli in 6/7 sampled points. Despite the low level of contamination by EHAZP in peridomiciliar soil and the absence of pathogenic protozoa in water, the AIL soil and water (due to the presence of fecal coliforms) are potential sources of infection for the population, indicating the need for improvements in sanitation and water treatment, in addition periodic treatment of the population with antiparasitic. PMID:26960764

  10. Ecological distribution of the shrimp camarao serrinha Artemesia longinaris (Decapoda, Penaeidae) in Fortaleza bay, Ubatuba, Brazil, in relation to abiotic factors

    OpenAIRE

    Fransozo, A.; Costa, R.C.; Castilho, A.L.; Mantelatto, F.L.

    2004-01-01

    The spatial and temporal distribution of the shrimp Artemesia longinaris in Fortaleza Bay, northern coast of Sao Paulo State, Brazil, in relation to abiotic factors, was studied. Shrimp samples were obtained on a monthly basis from November 1988 through October 1989 at seven pre-delimited transects on board of a fishing vessel supplied with double otter trawl nets. Depth was recorded at the midpoint of each transect; water and sediment samples were taken to measure salinity, temperature, text...

  11. Sodium and Potassium Absorption and Distribution in Relation to Growth and Internal Potassium Use Efficiency of K-efficient and -inefficient rice genotypes

    Institute of Scientific and Technical Information of China (English)

    2001-01-01

    A pot experiment with two rice (Oriza sativa L.) genotypes differing in internal potassium use efficiency (IKUE) was conducted under different sodium (Na) and potassium (K) levels. Adding NaC1 at a proper level enhanced rice vegetative growth and increased grain yield and IKUE under low potassium. Addition of higher rate of NaC1 had a negative effect on the growth of the K-efficient rice genotype, but did not for the K-inefficient genotype. Under low-K stress, higher NaC1 decreased IKUE of the K-efficient rice genotype but increased IKUE for the K-inefficient genotype. At tillering stage and under low-K stress, adding NaCl increased K and Na contents and decreased the ratio of K/Na for both genotypes. At harvesting stage under low-K stress, adding NaG1 increased K and Na contents and K/Na ratio for the K-efficient genotype but decreased the K/Na ratio for the K-inefficient genotype. The accumulated Na was mostly deposited in the roots and sheaths. At tillering stage, the K and Na contents and the K/Na ratios in different parts for both genotypes decreased in the following sequence: K+ in sheaths > K+ in blades > K+ in roots; Na+ in roots > Na+ in sheaths > Na+ in blades; and K/Na in sheaths 》 K/Na in roots. The K-efficient genotype had a lower K/Na ratio in roots and sheaths than the K-inefficient genotype under low-K stress. At harvesting stage, K and Na contents in grains were not affected, whereas K/Na ratio in the rice straws was increased for the K-efficient genotype but decreased for the K-inefficient genotype by Na addition. However, this was not the case under K sufficient condition.

  12. Diversity and distribution of oligochaetes in tropical forested streams, southeastern Brazil

    Directory of Open Access Journals (Sweden)

    Luciana Falci Theza Rodrigues

    2015-01-01

    Full Text Available The distribution and beta diversity of oligochaete assemblages were investigated in different spatial scales to verify the influence of environmental factors and geographic distance on their structure. Two types of mesohabitats (riffles and pools were sampled in eight first-order streams located in four preservation areas (Poço D’Anta Municipal Biological Reserve, Santa Cândida Municipal Biological Reserve, Ibitipoca State Park and a private farm called Fazenda Floresta and two Atlantic Forest phytophysiognomies (Seasonal Semideciduous Forest and Rocky Field. Variations in the taxon richness, abundance and composition of the oligochaete assemblages occurred between streams and phytophysiognomies, but not between riffles and pools in the same stream. Low beta diversity values were found and both turnover and nestedness contributed similarly in the environments studied, which could have occurred because of the high capability of oligochaetes to adapt to different environmental conditions. Although the canonical correspondence analysis explained 85.5% of the data (first three axes, the partial Mantel test showed greater influence of geographic distance on the faunal composition than the environmental variables measured. Simple linear regression confirmed this result and showed that the decay of similarity increased with distance between streams. The information from this study sheds light on how environmental and spatial factors determine the variation in the distribution and diversity of oligochaetes in forested low-order streams.

  13. Spatial distribution of fish assemblages along environmental gradients in the temporary ponds of Northern Pantanal, Brazil

    Directory of Open Access Journals (Sweden)

    Karina K. Tondato

    2013-02-01

    Full Text Available Ten temporary ponds in northern Pantanal were studied in July 2006 to explore whether a spatial distribution pattern existed in the composition of fish assemblages, and to identify which environmental variables determined their distribution. The existence of any spatial pattern was tested using the multivariate Mantel correlogram, while the influence of environmental variables was quantified by a Canonical Correspondence Analysis (CCA. A total of 8735 individuals was sampled from 29 species, predominantly represented by Hyphessobrycon elachys and Serrapinnus calliurus. Composition of fish assemblages varied among ponds, but this variation had no significant spatial pattern for any of the distance classes considered, thus indicating that the species composition varied independently of the distance between ponds. This suggests that stochastic dispersal processes did not influence the spatial structure of species, as predicted by the neutral theory. Conversely, species composition in the ponds was determined by variables that included depth, macrophyte richness and cover. Species such as Markiana nigripinnis, Crenicichla vittata and Moenkhausia sanctaefilomenae occurred in deeper waters, while Parauchenipterus striatulus, Eigenmannia trilineata and Psellogrammus kennedyi were mainly associated with greater richness and macrophyte cover, as already demonstrated by the niche theory applied in ponds which tended to have similar characteristics and a similar fish composition.

  14. Two Distinct Hepatitis C Virus Genotype 1a Clades Have Different Geographical Distribution and Association With Natural Resistance to NS3 Protease Inhibitors.

    Science.gov (United States)

    De Luca, Andrea; Di Giambenedetto, Simona; Lo Presti, Alessandra; Sierra, Saleta; Prosperi, Mattia; Cella, Eleonora; Giovanetti, Marta; Torti, Carlo; Caudai, Cinzia; Vicenti, Ilaria; Saladini, Francesco; Almi, Paolo; Grima, Pierfrancesco; Blanc, Pierluigi; Fabbiani, Massimiliano; Rossetti, Barbara; Gagliardini, Roberta; Kaiser, Rolf; Ciccozzi, Massimo; Zazzi, Maurizio

    2015-04-01

    Background.  Hepatitis C virus (HCV) genotype 1 is the most prevalent worldwide. Subtype 1a, compared with 1b, shows lower response rates and higher propensity to select for drug resistance to NS3 and selected NS5A and nonnucleoside NS5B inhibitors. Two distinct clades of subtype 1a have been described. Methods.  Using Bayesian methodology, we performed a time-scaled phylogeny reconstruction of clade separation and characterized the geographic distribution, phylodynamics, and association with natural resistance variants of NS3 sequences from 362 patients carrying subtype 1a HCV. Results.  All sequences segregated in 2 clearly distinct clades. Clade I showed an earlier origin from the common ancestor compared with clade II. Clade I virus was more prevalent in non-European countries, represented mostly by United States, compared with European (75.7% vs 49.3%; P < .001). The prevalence of the natural NS3 variant Q80K, associated with resistance to the macrocyclic protease inhibitor simeprevir, was detected in 51.6% of clade I and 0% of clade II (P < .001); clade I showed a lower genetic barrier for Q80K, whereas no sign of selective pressure at any protease inhibitor resistance-associated codon was detected. Conclusions.  Hepatitis C virus subtype 1a clades have a clearly different distribution in Europe and the United States, and the natural resistance mutation Q80K is exclusively associated with clade I. PMID:26213689

  15. Thermal Niches of Two Invasive Genotypes of the Wheat Curl Mite Aceria tosichella: Congruence between Physiological and Geographical Distribution Data

    Science.gov (United States)

    2016-01-01

    The wheat curl mite (WCM), Aceria tosichella Keifer, is a major pest of cereals worldwide. It is also a complex of well-defined genetic lineages with divergent physiological traits, which has not been accounted for in applied contexts. The aims of the study were to model the thermal niches of the two most pestiferous WCM lineages, designated MT-1 and MT-8, and to assess the extent to which temperature determines the distribution of these lineages. WCM population dynamics were modeled based on thermal niche data from March to November on the area of Poland (>311,000 km2). The most suitable regions for population development were predicted and compared to empirical field abundance data. Congruence between modeled parameters and field data for mite presence were observed for both WCM lineages although congruence between modeled thermal suitability and mite field abundance was observed only for MT-8. Thermal niche data for MT-1 and MT-8 provide biological insights and aid monitoring and management of WCM and the plant viruses it vectors. The presented models accurately estimate distributions of WCM and can be incorporated into management strategies for both current and predicted climate scenarios. PMID:27123590

  16. Serotype Distribution and Antimicrobial Resistance of Streptococcus pneumoniae prior to Introduction of the 10-Valent Pneumococcal Conjugate Vaccine in Brazil, 2000-2007: Pneumococcal Serotypes Prior to Conjugate Vaccine Introduction

    OpenAIRE

    Menezes, Ana Paula de O.; Campos, Leila C.; dos Santos, Milena S.; Azevedo, Jailton; dos Santos, Renan Cardoso Nery; Carvalho, Maria da Gloria S.; Beall, Bernard W.; Martin, Stacey W.; Salgado, Katia; Reis, Mitermayer G.; Ko, Albert I.; Reis, Joice N

    2010-01-01

    This study describes the serotype distribution and antibiotic resistance patterns among 397 S. pneumoniae meningitis case isolates recovered in Salvador, Brazil, during the period of 2000-2007, before introduction of the 10-valent pneumococcal conjugate vaccine.

  17. TRI12 based quantitative real-time PCR assays reveal the distribution of trichothecene genotypes of F. graminearum and F. culmorum isolates in Danish small grain cereals.

    Science.gov (United States)

    Nielsen, L K; Jensen, J D; Rodríguez, A; Jørgensen, L N; Justesen, A F

    2012-07-16

    Quantitative real-time PCR assays, based on polymorphisms in the TRI12 gene of the trichothecene pathway, were developed to identify and quantify the trichothecene genotypes producing 3-acetyl-deoxynivalenol (3ADON), 15-acetyl-deoxynivalenol (15ADON) or nivalenol (NIV) in the Fusarium graminearum species complex, Fusarium culmorum, Fusarium cerealis and Fusarium pseudograminearum. These assays were applied on a total of 378 field samples of cereal grain of wheat, barley, triticale, rye and oats collected from 2003 to 2007 to study the trichothecene genotype composition in Danish cereals. The three genotypes, 3ADON, 15ADON and NIV were found in all five cereal species, great annual variation in the occurrence of the trichothecene genotypes was evident with considerable variation between the samples. 3ADON was the dominant genotype in barley, triticale, rye and oats while 15ADON was most dominant in wheat. The NIV genotype was found at low levels in most samples. Study of genotype composition within the Danish F. graminearum and F. culmorum population was based on principal component analysis (PCA). PCA revealed that the dominating genotype of F. graminearum in wheat is 15ADON. For barley, the PCA analysis indicated that the F. graminearum population consisted of all three genotypes, and in triticale, the F. graminearum population consisted mainly of 15ADON genotype. F. culmorum/F. cerealis showed correlation to the NIV genotype in wheat and triticale but not in barley. F. culmorum/F. cerealis also showed some correlation to 3ADON especially in wheat and triticale. Selected wheat and barley samples from 1957 to 2000 showed low amounts of F. graminearum and F. culmorum in general but with a dominance of the 3ADON genotype. 15ADON was not detected in these samples, except for very low amounts in the sample representing the years from 1997 to 2000. Detection of low amounts of the 15ADON genotype in these historical samples and the relatively high amounts of 15ADON

  18. Radiopharmacy education in Brazil

    OpenAIRE

    Ralph Santos-Oliveira; Marta De Souza Albernaz

    2014-01-01

    The number of schools of pharmacy has been increasing each year in Brazil. From 2002 to 2013 over 300 new schools were opened in Brazil with a final number of 415 schools of pharmacy in operation around the country. Of these schools, only 28 schools offer a course in radiopharmacy (7.77%). However, the demand for such trained professionals has grown exponentially in Brazil, especially following amendment 49 (February 2006) that broke the monopoly on the production, distribution, and marketing...

  19. Global distribution of polymorphisms associated with delayed Plasmodium falciparum parasite clearance following artemisinin treatment: genotyping of archive blood samples.

    Science.gov (United States)

    Murai, Kenji; Culleton, Richard; Hisaoka, Teruhiko; Endo, Hiroyoshi; Mita, Toshihiro

    2015-06-01

    The recent emergence and spread of artemisinin-resistant Plasmodium falciparum isolates is a growing concern for global malaria-control efforts. A recent genome-wide analysis study identified two SNPs at genomic positions MAL10-688956 and MAL13-1718319, which are linked to delayed clearance of parasites following artemisinin combination therapy (ACT). It is expected that continuous artemisinin pressure will affect the distribution of these SNPs. Here, we investigate the worldwide distribution of these SNPs using a large number of archived samples in order to generate baseline data from the period before the emergence of ACT resistance. The presence of SNPs in MAL10-688956 and MAL13-1718319 was assessed by nested PCR RFLP and direct DNA sequencing using 653 global P. falciparum samples obtained before the reported emergence of ACT resistance. SNPs at MAL10-688956 and MAL13-1718319 associated with delayed parasite clearance following ACT administration were observed in 8% and 3% of parasites, respectively, mostly in Cambodia and Thailand. Parasites harbouring both SNPs were found in only eight (1%) isolates, all of which were from Cambodia and Thailand. Linkage disequilibrium was detected between MAL10-688956 and MAL13-1718319, suggesting that this SNP combination may have been selected by ACT drug pressure. Neither of the SNPs associated with delayed parasite clearance were observed in samples from Africa or South America. Baseline information of the geographical difference of MAL10-688956 and MAL13-1718319 SNPs provides a solid basis for assessing whether these SNPs are selected by artemisinin-based combination therapies.

  20. Identification of Cryptosporidium species and genotypes in dairy cattle in Brazil Identificação de espécies e genótipos de Cryptosporidium em bovinos leiteiros no Brasil

    Directory of Open Access Journals (Sweden)

    Flavio Medeiros Paz e Silva

    Full Text Available In this study, we identified Cryptosporidium species and genotypes present in dairy cattle in the central region of São Paulo state, Brazil. Fecal specimens were collected from 200 animals (100 calves and 100 cows in ten dairy farms. Fecal samples were examined using microscopic examination (ME, enzyme immunoassay (EIA and polymerase chain reaction (PCR. Cryptosporidium species and genotypes were determined by restriction fragment length polymorphism (RFLP or DNA sequencing analysis of the SSU-rRNA and GP60 genes. The occurrence of Cryptosporidium spp. infection was 14% (28/200. The occurrence in calves (26% was significantly higher than in cows (2%. Of the 27 Cryptosporidium-positive specimens submitted to genotyping, C. andersoni was identified in 23 (85.1%, C. bovis in three (11.1%, and the zoonotic C. parvum subtype IIaA15G2R1 in one (3.7%. The study demonstrates that Cryptosporidium spp. infection was common and widespread in dairy cattle in this region and that calves have a high prevalence of C. andersoni. Furthermore, the presence of C. parvum subtype IIaA15G2R1 indicates that dairy calves from this region should be considered a potential source of zoonotic Cryptosporidium oocysts.No presente estudo foram identificadas espécies e genótipos de Cryptosporidium originadas de bovinos leiteiros na região central do estado de São Paulo, Brasil. Amostras fecais foram coletadas de 200 animais (100 bezerros e 100 vacas em 10 propriedades leiteiras. As amostras foram examinadas utilizando os métodos de microscopia óptica (MO, ensaio imunoenzimático (EI e reação em cadeia da polimerase (PCR. As espécies e genótipos de Cryptosporidium foram determinados pelo método de polimorfismo no tamanho dos fragmentos de restrição (RFLP ou sequenciamento dos genes SSU-rRNA e GP60. A infecção por Cryptosporidium spp. teve ocorrência de 14% (28/200. A ocorrência em bezerros (26% foi significativamente maior do que em vacas (2%. Do total de 27

  1. TRI12 based quantitative real-time PCR assays reveal the distribution of trichothecene genotypes of F. graminearum and F. culmorum isolates in Danish small grain cereals

    DEFF Research Database (Denmark)

    Nielsen, L.K.; Jensen, Jens Due; Rodriguez, A.;

    2012-01-01

    species complex, Fusarium culmorum, Fusarium cerealis and Fusarium pseudograminearum. These assays were applied on a total of 378 field samples of cereal grain of wheat, barley, triticale, rye and oats collected from 2003 to 2007 to study the trichothecene genotype composition in Danish cereals. The three...... genotypes, 3ADON, 15ADON and NIV were found in all five cereal species, great annual variation in the occurrence of the trichothecene genotypes was evident with considerable variation between the samples. 3ADON was the dominant genotype in barley, triticale, rye and oats while 15ADON was most dominant...

  2. Vertical distribution of benthic marine organisms on rocky coasts of the Fernando de Noronha archipelago (Brazil

    Directory of Open Access Journals (Sweden)

    Verena Rapp de Eston

    1986-01-01

    Full Text Available Qualitative samples of flora and fauna were collected in several points, from the supralittoral fringe down to 30 m, using mainly aqualungs. Species distribution and abundance were determined along four transects, up to 200 m long, in selected places. Samples were taken from 25 x 25 cm quadrats positioned at fixed intervals along the transects down to a variable depth, depending on algal percentage cover and their substrate. The intertidal zone was populated essentially by molluscs. The subtidal was covered predominantly by macroalgae. Montastrea cavernosa, Siderastrea stellata and Mussismillia hispida were the only animal species with a significant percentage cover within the quadrats. Dictyopteris justii, D. plagiograma, Dictyota cervicornis, D. linearis, D. mertensii, Sargassum platycarpum, Sargassum sp, and Stypopodium zonale were the plants with the highest biomass and/or percentage cover, along with Amphiroa fragilissima, Caulerpa verticillata, and crustose corallines. The scarcity of sea urchins, along with the dominance of algae known to produce chemical feeding deterrents or to have a calcareous nature, points towards a strong pressure by predators, with fishes probably playing a key role in the maintenance of the structure of this subtidal community.

  3. Spatial and temporal distribution of ichthyoplankton in the upper Uruguay river, Brazil

    Directory of Open Access Journals (Sweden)

    Samara Hermes-Silva

    2009-08-01

    Full Text Available The distribution and abundance of fish eggs and larvae was analyzed in three sections of the Upper Uruguay river, in a stretch of 290 km. Samples were collected monthly from October, 2001 to March, 2002 during 48-h cycles at 6-h intervals between each sampling. Surface and bottom samples were collected with a 0.5-mm mesh cylindroconical net. Fishes from the Upper Uruguay river were reproductively active mainly from October to January, and this activity was more intense at the Ligeiro and Chapecó tributaries and Chapecó main river. It was observed that the tributaries are important spawning grounds and larval nursery sites, indicating the importance of preserving such environments.Foram analisadas a distribuição e abundância de ovos e larvas de peixes em três seções do Alto rio Uruguai, num trecho de 290 km. As coletas foram realizadas mensalmente no período de outubro de 2001 a março de 2002, durante um ciclo de 48 horas com intervalos de 6 horas entre as amostragens. Foram feitas coletas de superfície e fundo, nas margens e no canal central do rio, utilizando-se redes de plâncton do tipo cônicocilíndricas de malha 0,5mm. As maiores ocorrências foram verificadas entre os meses de outubro a janeiro, sendo que a atividade reprodutiva foi mais intensa nos tributários Ligeiro e Chapecó e no rio Uruguai, na foz do rio Chapecó. Foi observado neste estudo que alguns tributários se destacam como locais de desova e desenvolvimento de larvas de peixe, indicando a importância de se preservar estes ambientes.

  4. Vertical and horizontal distribution of pollination systems in cerrado fragments of central Brazil

    Directory of Open Access Journals (Sweden)

    Fernanda Quintas Martins

    2007-05-01

    Full Text Available In fragments of the cerrado, we determined the frequency of pollination systems and analyzed their spatial distribution. We placed 38 transects, sampling 2,280 individuals and 121 species. As expected in Neotropical regions, bee-pollination was the most frequent pollination system. We found a decrease in the frequency of plants pollinated by beetles towards the fragment interior. Similarly, we found significant variation in relation to height just for the bats; there was an increase in the frequency of plants pollinated by bats towards the higher heights. In general, we found no horizontal and vertical variation in the pollination systems, probably as consequence of the more open physiognomy of the cerrado vegetation.As principais pressões seletivas nas estratégias de polinização originam principalmente do ambiente em que plantas ocorrem, como subdossel, dossel, borda ou interior de um fragmento. Diferentes condições ambientais aumentam as diferenças entre os nichos ecológicos e podem implicar diferenças nas proporções dos sistemas de polinização. Em fragmentos de cerrado, determinamos a freqüência dos sistemas de polinização e analisamos sua distribuição espacial. Lançamos 38 transecções aleatoriamente, amostrando 2.280 indivíduos e 121 espécies. Como esperado para regiões neotropicais, a polinização por abelhas foi o sistema de polinização mais freqüente. Encontramos uma diminuição na freqüência de plantas polinizadas por besouros em direção ao interior do fragmento. De modo similar, encontramos uma variação significativa em relação à altura somente para os morcegos, havendo um aumento na freqüência de plantas em direção a alturas mais altas. Em geral, não encontramos variações horizontais e verticais nos sistemas de polinização, provavelmente, como conseqüência da fisionomia mais aberta de cerrado.

  5. Distributed deformation structures in shallow water carbonates subsiding through a simple stress field (Jandaira Formation, NE Brazil)

    Science.gov (United States)

    Bertotti, Giovanni; Bisdom, Kevin; Bezerra, Hilario; Reijmer, John; Cazarin, Carol

    2016-04-01

    Despite the scarcity of major deformation structures such as folds and faults, the flat-lying, post-rift shallow water carbonates of the Jandaira Formation (Potiguar Basin, NE Brazil) display well-organized fracture systems distributed of tens of km2. Structures observed in the outcropping carbonates are sub-vertical, generally N-S trending mode I and hybrid veins and barren fractures, sub-vertical roughly E-W trending stylolites and sub-horizontal stylolites. These features developed during subsidence in a simple and constant stress field characterized by, beside gravity, a significant horizontal stress probably of tectonic origin. The corresponding depth curves have different origin and slopes and, therefore, cross each other resulting in position of the principal stresses which change with depth. As a result, the type and amount of fractures affecting subsiding rocks change despite the fact that the far-field stresses remain constant. Following early diagenesis and porosity elimination in the first 100-200m depth, Jandaira carbonates experienced wholesale fracturing at depths of 400-800m resulting in a network of NNW-NE trending fractures partly organized in conjugate sets with a low interfault angle and a sub-vertical intersection, and sub-vertical stylolites roughly perpendicular to the fractures. Intense fluid circulation was activated as a consequence through the carbonates. With increasing subsidence, sub-horizontal stylolites formed providing calcite which precipitated in the open fractures transforming them in veins. The Jandaira formation lost thereby the permeability it had reached during the previous stage. Because of the lack of major deformation, the outcrops of the Jandaira Formation is an excellent analog for carbonate reservoirs in the Middle East, South Atlantic and elsewhere.

  6. HPV genotypes in invasive cervical cancer in Danish women

    DEFF Research Database (Denmark)

    Kirschner, Benny; Junge, Jette; Holl, Katsiaryna;

    2013-01-01

    Human papillomavirus (HPV) genotype distribution in invasive cervical cancers may differ by geographic region. The primary objective of this study was to estimate HPV-genotype distribution in Danish women with a diagnosis of invasive cervical cancer....

  7. Sodium and Potassium Absorption and Distribution in Relation to Growth and Internal Potassium Use Efficiency of K—efficient and—inefficient rice genotypes

    Institute of Scientific and Technical Information of China (English)

    LIUJIANXIANG; YANGXIAOE; 等

    2001-01-01

    A pot experiment with two rice(Oriza saiva L.)genotypes differing in internal potassium use efficiency (IKUE) was condcucted under different sodium( Na) and potassium(k) levels.Addin NaCl at a proper level enhanced rice vegetative growth and increased grain yield and IKUE under low potassium Addition of higher rate of NaCl had a negative effect on the growth of the K-efficient rice genotype,but did not fro the K-inefficient genotype.Under low-K stress,higher NaCl decreased IKUE of the K-efficient rices genotype but increased IKUE for the K-inefficient genotype,At tillering stage and under low-K stress,adding NaCl increased K and Na contents and decreased the ration of K/Na both genotypes ,At harvesting stage under low-K stress,adding NaCl increased K and Na contents and K/Na ratio for the K-efficient genotype but decreased the K/Na ratio for the K=inefficient genotype.The accumulated Na was mostly deposited in the roots and sheaths ,At tillering stage,the K and Na contents and the K/Na ratios in different parts for both genotypes decreased in the following sequence:K+ in sheaths >K+ in blades>K+ in roots;Na+ in roots >Na+ in sheaths>Na+ in blades;and K/Na in sheaths ≥K/Na in roots.The K-effcient genotype had a lower K/Na ratio in roots and sheaths than the K-inefficient genotype under low-K stress,At harvesting stage,K and Na contents in grains were not affected ,hereas K/Na ratio in the rice straws was increased for the K-efficient genotype but decreased for the K-inefficient genotype by Na addition.However,this was not the case under K sufficient condition.

  8. Distribution of hepatitis B infection in Brazil: the epidemiological situation at the beginning of the 21 st century.

    Science.gov (United States)

    Souto, Francisco José Dutra

    2016-02-01

    Brazil was formerly considered a country with intermediate hepatitis B endemicity, with large heterogeneity between Brazilian regions and areas of high prevalence, especially in the Amazon basin. Systematic vaccination of children was initiated in 1998. Between 2004 and 2009, a large population-based study reported decreased prevalence in all regions of Brazil. This review analyzed the current hepatitis B epidemiological situation in Brazil through a systematic search of the scientific literature in MEDLINE, LILACS, and CAPES thesis database, as well as disease notifications to the Information System for Notifiable Diseases. The search strategy identified 87 articles and 13 theses, resulting in 100 total publications. The most recent results indicate reduced hepatitis B prevalence nationwide, classifying Brazil as having low endemicity. Most studies showed HBV carrier prevalence less than 1%. However, there are still isolated regions with increased prevalence, particularly the Amazon, as well as specific groups, such as homeless people in large cities and isolated Afro-descendant communities in the center of the country. This review alsao detected successful vaccination coverage reported in a few studies around the country. The prevalence of anti-HBs alone ranged from 50% to 90%. However, isolated and distant localities still have low coverage rates. This review reinforces the downward trend of hepatitis B prevalence in Brazil and the need to intensify vaccination strategies for young people and adults in specific regions with persisting higher HBV infection prevalence. PMID:26689276

  9. Distribution of hepatitis B infection in Brazil: the epidemiological situation at the beginning of the 21 st century

    Directory of Open Access Journals (Sweden)

    Francisco José Dutra Souto

    2015-01-01

    Full Text Available Abstract Brazil was formerly considered a country with intermediate hepatitis B endemicity, with large heterogeneity between Brazilian regions and areas of high prevalence, especially in the Amazon basin. Systematic vaccination of children was initiated in 1998. Between 2004 and 2009, a large population-based study reported decreased prevalence in all regions of Brazil. This review analyzed the current hepatitis B epidemiological situation in Brazil through a systematic search of the scientific literature in MEDLINE, LILACS, and CAPES thesis database, as well as disease notifications to the Information System for Notifiable Diseases. The search strategy identified 87 articles and 13 theses, resulting in 100 total publications. The most recent results indicate reduced hepatitis B prevalence nationwide, classifying Brazil as having low endemicity. Most studies showed HBV carrier prevalence less than 1%. However, there are still isolated regions with increased prevalence, particularly the Amazon, as well as specific groups, such as homeless people in large cities and isolated Afro-descendant communities in the center of the country. This review alsao detected successful vaccination coverage reported in a few studies around the country. The prevalence of anti-HBs alone ranged from 50% to 90%. However, isolated and distant localities still have low coverage rates. This review reinforces the downward trend of hepatitis B prevalence in Brazil and the need to intensify vaccination strategies for young people and adults in specific regions with persisting higher HBV infection prevalence.

  10. Characteristics of the distribution of doctors in the Mais Médicos (More Doctors) Program in the states of Brazil's Northeast.

    Science.gov (United States)

    Nogueira, Priscila Tamar Alves; Bezerra, Adriana Falangola Benjamin; Leite, Antonio Flaudiano Bem; Carvalho, Islândia Maria de Sousa; Gonçalves, Rogério Fabiano; Brito-Silva, Keila Silene de

    2016-09-01

    Inequalities in access to health, reflecting shortages and inadequate geographical distribution of health professionals, have been indicated as a challenge for Brazil. This paper analyzes the geographical distribution of professionals of the Mais Médicos Program allocated in the Northeastern Region of Brazil, through a descriptive cross-sectional study. Secondary data provided by the Health Ministry were used, and thematic maps of distribution of doctors in the Region were prepared. Data on 4,716 doctors who became members of health teams in 1,294 municipalities in six Indigenous Health Districts in the years 2013 and 2014 were analyzed. The greater part of the municipalities of the Region received between one and five doctors. The municipalities most benefited had, at least, 20% of their population in extreme poverty. 99.9% of the doctors were allocated in a Health Center or Primary Healthcare Unit. The majority were women (57%), predominantly of the 45-49 age group (24%). In spite of the advances achieved by the Program - such as distribution of the doctors in locations with greater vulnerability - some States continue to have significant shortages of healthcare.

  11. 腮腺肿瘤中HPV37种亚型的分布%Distribution of 37 Kinds of Human Papillomavirus Genotypes in Parotid Gland Tumors

    Institute of Scientific and Technical Information of China (English)

    王妍; 陈晓平; 薛晓成; 张燚; 陈群; 张竞飞

    2012-01-01

    目的 探讨腮腺肿瘤(Parotid Gland Tumor)与人乳头状瘤病毒(Human Papillomavirus,HPV)感染及其亚型的相关性.方法 采用核酸分子快速导流杂交基因芯片分型技术(Hybri Max)对59例腮腺肿瘤石蜡包埋组织标本进行37种HPV亚型检测,并以20例健康志愿者的正常口腔黏膜组织为对照.结果 腮腺肿瘤石蜡包埋组织标本HPV总检出率为57.6%(34/59),对照组均为阴性(0/20),两组比较差异有统计学意义(χ2=20.234,P<0.05);腮腺良性、恶性肿瘤组织中HPV阳性率分别为59.6%(31/52)和42.9%(3/7),两组HPV阳性率比较差异无统计学意义(χ2=0.189,P>0.05);腮腺肿瘤组中HPV感染亚型以高危亚型为主(80.7%),其中以HPV16、18、52型为主(61.4%);腮腺肿瘤组中HPV单一亚型、多重亚型均可感染,但以混合亚型感染为主(52.9%).结论 腮腺肿瘤的发生可能与HPV感染有相关性.腮腺肿瘤中HPV高、低危亚型均可感染,以高危亚型HPV16、18、52型感染为主.%Objective To investigate the distribution of HPV subtypes in the pariotid gland tumors and to e-valuate the relationship between HPV and pariotid gland tumors. Methods 37 HPV subtypes were detected in paraffin - embedded tissues of 59 cases with parotid gland tumors by flow - through hybridization and gene chip( Hybrimax) technology. 20 cases of normal oral mucosa were used as controls. Results HPV general positive rate of pariotid gland tumors was 57. 6% (34/59). The rate in control group were all negative (0/ 20). The HPV -positive rates of benign tumor and malignent tumor of pariotid gland were 59.6% (31/52) and 42. 9% (3/7) . The high - risk HPV(80. 7% ) was the most prevalent type in the group of parotid gland tumors and high - risk HPV - 16/18/52 were mainly prevalent types (61. 4%). Both single - HPV and multi - HPV genotype existed in the HPV - positive samples and most of cases (52. 9% ) were infected with multi - HPV genotype. Conclusion Incidence of parotid gland

  12. HBV genotypic variability in Cuba.

    Science.gov (United States)

    Loureiro, Carmen L; Aguilar, Julio C; Aguiar, Jorge; Muzio, Verena; Pentón, Eduardo; Garcia, Daymir; Guillen, Gerardo; Pujol, Flor H

    2015-01-01

    The genetic diversity of HBV in human population is often a reflection of its genetic admixture. The aim of this study was to explore the genotypic diversity of HBV in Cuba. The S genomic region of Cuban HBV isolates was sequenced and for selected isolates the complete genome or precore-core sequence was analyzed. The most frequent genotype was A (167/250, 67%), mainly A2 (149, 60%) but also A1 and one A4. A total of 77 isolates were classified as genotype D (31%), with co-circulation of several subgenotypes (56 D4, 2 D1, 5 D2, 7 D3/6 and 7 D7). Three isolates belonged to genotype E, two to H and one to B3. Complete genome sequence analysis of selected isolates confirmed the phylogenetic analysis performed with the S region. Mutations or polymorphisms in precore region were more common among genotype D compared to genotype A isolates. The HBV genotypic distribution in this Caribbean island correlates with the Y lineage genetic background of the population, where a European and African origin prevails. HBV genotypes E, B3 and H isolates might represent more recent introductions.

  13. Avaliação de genótipos de pimentão no período de inverno, em Araguari, MG Genotypic evaluation of sweet pepper in the winter season in Araguari, MG, Brazil

    Directory of Open Access Journals (Sweden)

    José Ricardo Peixoto

    1999-10-01

    Full Text Available Desenvolveu-se um trabalho de pesquisa na Fazenda Jordão em Araguari, MG (18º38'30" S e 48º11'18" O, na época do inverno, com o objetivo de verificar o desempenho agronômico de genótipos de pimentão. Utilizou-se o delineamento experimental de blocos casualizados, com 17 tratamentos (17 genótipos e quatro repetições. A parcela experimental foi constituída por uma fileira com 30 plantas, no espaçamento de 1,00 x 0,45 m. Efetuou-se um total de dez colheitas sendo a primeira feita aos 28 de julho de 1996 (77 dias após o transplante e a última em 9 de novembro de 1996. Vários híbridos F1 apresentaram boas características agronômicas, podendo ser plantadas na região, no período de inverno, principalmente Lygia, Magali e Magali R, Acuário, Luis, Fresco (BS 50-30 e Sambor. O híbrido Lygia F1 destacou-se em produtividade, seguido por Acuário F1 e Magali F1, e também em número total de frutos, porém apresentou formato de fruto próximo ao quadrado e classificação inferior de frutos extra AA. Os genótipos Acuário F1, Sambor F1, Fresco (BS 50-30 F1, Luis F1 e All Big se destacaram em frutos tipo extra AA, enquanto Magna Super, Nacional AG-506, Itaipu e Hércules AG-672, apresentaram maior número de frutos tipo extra A.An experimental field was carried out in the Jordão farm, located in Araguari, MG, Brazil, at 18º38'30" S and 48º11'18" W, during the winter season, with the purpose of evaluating the agronomic potential of sweet pepper cultivars. The experimental design was in randomized blocks with 17 treatments (17 genotypes with four replications. The experimental plots were made up each of one line with 30 plants, with 1 m of space between lines and 0,45 m between plants. A total of ten harvestings were made starting on July 28, 1996 (77 days after transplanting and ending on November 9, 1996. Several F1 hybrids as Lygia F1, Magali F1, Magali R F1, Acuário F1, Luis F1, Fresco (BS 50-30 F1 and Sambor F1 showed good

  14. Three new species and new distributional records of Oecetis McLachlan 1877 (Trichoptera: Leptoceridae: Leptocerinae) from Brazil.

    Science.gov (United States)

    Henriques-Oliveira, Ana Lucia; Dumas, Leandro Lourenço; Nessimian, Jorge Luiz

    2014-01-01

    The genus Oecetis is widespread over the world, being most diverse in tropical areas, especially in the Australian Region. Of about 400 described species, only 34 occur in the Neotropical Region. Herein, we describe and illustrate three new species of Oecetis from Brazil: Oecetis angelae sp. nov., O. danielae sp. nov. and O. iara sp. nov. Furthermore, O. doesburgi, O. dominguezi, and O. knutsoni are recorded for the first time in Brazil. Also, we provide new state records for 7 species: O. amazonica, O. connata, O. excisa, O. fibra, O. iguazu, O. inconspicua, and O. paranensis. PMID:24872297

  15. Three new species and new distributional records of Oecetis McLachlan 1877 (Trichoptera: Leptoceridae: Leptocerinae) from Brazil.

    Science.gov (United States)

    Henriques-Oliveira, Ana Lucia; Dumas, Leandro Lourenço; Nessimian, Jorge Luiz

    2014-01-07

    The genus Oecetis is widespread over the world, being most diverse in tropical areas, especially in the Australian Region. Of about 400 described species, only 34 occur in the Neotropical Region. Herein, we describe and illustrate three new species of Oecetis from Brazil: Oecetis angelae sp. nov., O. danielae sp. nov. and O. iara sp. nov. Furthermore, O. doesburgi, O. dominguezi, and O. knutsoni are recorded for the first time in Brazil. Also, we provide new state records for 7 species: O. amazonica, O. connata, O. excisa, O. fibra, O. iguazu, O. inconspicua, and O. paranensis.

  16. Fauna of Simuliidae (Diptera from the state of Espírito Santo, Brazil: distribution, new records and list of species

    Directory of Open Access Journals (Sweden)

    Karina Bertazo

    2013-03-01

    Full Text Available Fauna of Simuliidae (Diptera from the state of Espírito Santo, Brazil: Distribution, new records and list of species. The fauna of Simuliidae in Brazil is composed of 87 species, 17 of which are recorded from the state of Espírito Santo. Entomological collections were carried out in 2010-2011 with the objective of increasing the knowledge of the species richness of this family in the state. Ninety three rivers and streams were sampled, each collection being carried out in a 50m transect. During the study period 30 species were collected, 13 of which represent new records for the state, 12 of the genus Simulium and one of the genus Lutzsimulium. Among these new state records one, Simulium lobatoi, also represents a new record from southeastern Brazil. The other newly recorded species are: Lutzsimulium hirticosta, Simulium distinctum, Simulium exiguum, Simulium oyapockense, Simulium botulibranchium, Simulium petropoliense, Simulium clavibranchium, Simulium rappae, Simulium minusculum, Simulium dinellii, Simulium ochraceum and Simulium scutistriatum.

  17. The distinct distribution and phylogenetic characteristics of dengue virus serotypes/genotypes during the 2013 outbreak in Yunnan, China: Phylogenetic characteristics of 2013 dengue outbreak in Yunnan, China.

    Science.gov (United States)

    Wang, Binghui; Yang, Henglin; Feng, Yue; Zhou, Hongning; Dai, Jiejie; Hu, Yunzhang; Zhang, Li; Wang, Yajuan; Baloch, Zulqarnain; Xia, Xueshan

    2016-01-01

    Since 2000, sporadic imported cases of dengue fever were documented almost every year in Yunnan Province, China. Unexpectedly, a large-scale outbreak of dengue virus (DENV) infection occurred from August to December 2013, with 1538 documented cases. In the current study, 81 dengue-positive patient samples were collected from Xishuangbanna, the southernmost prefecture of the Yunnan province, and 23 from Dehong, the westernmost prefecture of the Yunnan province. The full-length envelope genes were amplified and sequenced. Phylogenetic analysis revealed that nine strains (39.1%) and 14 strains (60.9%) from the Dehong prefecture were classified as genotype I of DENV-1 and Asian I genotype of DENV-2, respectively. All strains from Xishuangbanna were identified as genotype II of DENV-3. Bayesian coalescent analysis indicates that the outbreak originated from bordering southeastern Asian countries. These three epidemic genotypes were predicted to originate in Thailand and then migrate into Yunnan through different routes.

  18. Mendel: a simple excel workbook to compare the observed and expected distributions of genotypes/phenotypes in transgenic and knockout mouse crosses involving up to three unlinked loci by means of a χ2 test.

    Science.gov (United States)

    Montoliu, Lluís

    2012-06-01

    The analysis of transgenic and knockout mice always involves the establishment of matings with individuals carrying different loci, segregating independently, whose presence is expected among the progeny, according to a Mendelian distribution. The appearance of distorted inheritance ratios suggests the existence of unexpected lethal or sub-lethal phenotypes associated with some genotypes. These situations are common in a number of cases, including: testing transgenic founder mice for germ-line transmission of their transgenes; setting up heterozygous crosses to obtain homozygous individuals, both for transgenic and knockout mice; establishing matings between floxed mouse lines and suitable cre transgenic mouse lines, etc. The Pearson's χ(2) test can be used to assess the significance of the observed frequencies of genotypes/phenotypes in relation to the expected values, in order to determine whether the observed cases fit the expected distribution. Here, I describe a simple Excel workbook to compare the observed and expected distributions of genotypes/phenotypes in transgenic and knockout mouse crosses involving up to three unlinked loci by means of a χ(2) test. The file is freely available for download from my laboratory's web page at: http://www.cnb.csic.es/~montoliu/Mendel.xls .

  19. Distributed photovoltaic generation in Brazil: An economic viability analysis of small-scale photovoltaic systems in the residential and commercial sectors

    International Nuclear Information System (INIS)

    This paper examines the economic viability of small-scale, grid-connected photovoltaics in the Brazilian residential and commercial sectors after the introduction of the net metering regulation in April 2012. This study uses the discounted cash flow method to calculate the specific investment costs that are necessary for photovoltaic systems to be economically viable for each of the 63 distribution networks in Brazil. We compare these values to the system costs that are estimated in the comprehensive study on photovoltaics that was developed by the Brazilian Association of Electric and Electronic Industries (ABINEE). In our calculation, we utilize the current electricity tariffs, including fees and taxes, which we obtained through telephone interviews and publicly available information. We obtained a second important parameter by simulating PV-systems with the program PV⁎Sol at the distribution company headquarters' locations. In our base case scenario that reflects the current situation, in none of the distribution networks photovoltaics is economically viable in either the commercial or residential sectors. We improved the environment for grid-connected photovoltaics in our scenarios by assuming both lower PV-system costs and a lower discount rate to determine the effect on photovoltaics viability. - Highlights: • We calculate the economic viability of photovoltaics in the residential and commercial sectors in Brazil. • The PV⁎Sol simulations are carried out at the headquarter locations for the 63 distribution companies. • Currently in none of the distribution networks, photovoltaics is economically viable in either the commercial or residential sectors. • We analyze how the variation of the specific investment costs and of the discount rate affects the economic viability

  20. Hepatitis C virus genotypes in Pakistan: a systemic review

    Directory of Open Access Journals (Sweden)

    Ali Ijaz

    2011-09-01

    Full Text Available Abstract Background and aim Phylogenetic analysis has led to the classification of hepatitis C virus (HCV into 1-6 major genotypes. HCV genotypes have different biological properties, clinical outcome and response to antiviral treatment and provide important clues for studying the epidemiology, transmission and pathogenesis. This article deepens the current molecular information about the geographical distribution of HCV genotypes and subgenotypes in population of four provinces of Pakistan. 34 published papers (1996-2011 related to prevalence of HCV genotypes/serotypes and subgenotypes in Pakistan were searched. Result HCV genotype/s distribution from all 34 studies was observed in 28,400 HCV infected individuals in the following pattern: 1,999 (7.03% cases of genotype 1; 1,085 (3.81% cases of genotype 2; 22,429 (78.96% cases of genotype 3; 453 (1.59% cases of genotype 4; 29 (0.10% cases of genotype 5; 37 (0.13% cases of genotype 6; 1,429 (5.03% cases of mixed genotypes, and 939 (3.30% cases of untypeable genotypes. Overall, genotype 3a was the predominant genotype with a rate of 55.10%, followed by genotype 1a, 3b and mixed genotype with a rate of 10.25%, 8.20%, and 5.08%, respectively; and genotypes 4, 5 and 6 were rare. Genotype 3 occurred predominately in all the provinces of Pakistan. Second more frequently genotype was genotype 1 in Punjab province and untypeable genotypes in Sindh, Khyber Pakhtunkhwa and Balochistan provinces.

  1. Molecular characterization of group A rotavirus isolates obtained from hospitalized children in Salvador, Bahia, Brazil

    Directory of Open Access Journals (Sweden)

    Karina Serravalle

    2007-02-01

    Full Text Available Rotavirus is a major cause of infectious diarrhea in infants and young children. The objective of this study was to characterize the genotypes of Human Rotavirus found in children hospitalized with acute diarrhea in the Pediatric Hospital Prof. Hosannah de Oliveira of the UFBA in Salvador, Bahia, Brazil, during the years of 1999, 2000 and 2002. Fecal samples were analyzed (n=358 by methods EIARA and SDS-PAGE for detection of Rotavirus. Positive samples of one or two of these methods (n=168 were submitted to RT-PCR and Multiplex-Nested PCR to determine genotypes G and P. A hundred sixty-eight (46.9% samples were positive and 190 (53.1% negative. Only 17 (4.7% samples had divergent results. The distribution of genotypes G during the first year, showed that the genotype G9 was present in 96,8% of the analyzed samples, in the second year, it was responsible for 96% and in the third year, 88,1%. The characterization of genotypes P demonstrated that the genotype P1A[8] was the most outstanding in all years. In this study we discuss the benefit to control the genotypes of Rotavirus through the molecular characterization for the development of potential vaccines.

  2. White grubs (Coleoptera, Melolonthidae in the "Planalto Region", Rio Grande do Sul state, Brazil: Key for identification, species richness and distribution

    Directory of Open Access Journals (Sweden)

    Mariana A. Cherman

    2013-09-01

    Full Text Available White grubs (Coleoptera, Melolonthidae in the "Planalto Region", Rio Grande do Sul state, Brazil: Key for identification, species richness and distribution. The objective of this study was to survey the occurrence and geographic distribution of white grub species (Coleoptera, Melolonthidae in cultivated and non-cultivated fields of the "Planalto Region", Rio Grande do Sul state, Brazil and develop a key at genus-level. Twenty-eight species from 15 genera and three subfamilies were recorded: Dynastinae, Melolonthinae and Rutelinae. The species or genera recorded for the first time in the state are: Cyclocephala metrica, C. tucumana, Isonychus albicinctus, Liogenys bidenticeps, L. fusca, L. obesa and L. sinuaticeps, Paranomala violacea, as well as unidentified species of Amononyx, Dicrania, Leucothyreus, Macrodactylus, Plectris and Rhizogeniates. Among the species recorded, 23 were associated with winter crops. Only Cyclocephala metrica, Dyscinetus rugifrons, two species of Leucothyreus and one species of the tribe Sericini were not present in cultivated crop fields. Cyclocephala flavipennis and Diloboderus abderus occurred in most of the municipalities sampled, often associated with Plectris sp., C. modesta and C. putrida. The highest richness of melolonthids was concentrated in the northeast of the Planalto region.

  3. Richness, geographic distribution and ecological aspects of the fern community within the Murici Ecological Station in the state of Alagoas, Brazil

    Directory of Open Access Journals (Sweden)

    Anna Flora de Novaes Pereira

    2013-12-01

    Full Text Available We conducted a floristic survey of ferns within the Murici Ecological Station (remnant of the northeastern Atlantic Forest, located near the municipalities of Messias and Murici, in the state of Alagoas, Brazil. To increase knowledge of the ferns of Alagoas, we evaluated the species occurring in the study area in terms of richness, composition, geographic distribution, similarities with species in other Brazilian biomes, regional conservation status and ecological aspects. Data were obtained from field work conducted between March 2009 and September 2010. We identified 107 species of ferns, of which 19 represent new records for Alagoas. The richest families were Pteridaceae (29 species and Polypodiaceae (22 species. The richest genera were Adiantum (15 species and Thelypteris (9 species. Most of the species sampled are widely distributed throughout Brazil and the Americas. Within the context of the northeastern Atlantic Forest, 12 species were considered endangered. Concerning the ecological aspects, 88.8% of the species identified were herbaceous, 57.9% were terrestrial and 70.0% occurred in the forest interior.

  4. Distribution and abundance of Cladocera (Branchiopoda in the Paraíba do Sul River estuary, Rio de Janeiro, Brazil

    Directory of Open Access Journals (Sweden)

    José Mauro Sterza

    2006-12-01

    Full Text Available To characterize the cladoceran community of the Paraíba do Sul River estuary, located in the district of São João da Barra, Rio de Janeiro, Brazil, cladocerans were collected monthly in nine sampling stations from September 2002 until August 2003. Samples were obtained by subsurface tows using a plankton net with a 30 cm opening mouth and 70 micron mesh size, fitted with a mechanical flowmeter. Environmental parameters such as salinity and temperature were also obtained. Seventeen species of Cladocera were identified: Pseudoevadne tergestina, Penilia avirostris, Macrothrix triserialis, Moina micrura, Simocephalus kerhervei, Simocephalus vetalus, Simocephalus latirostris, Simocephalus serrulatus, Alona rectangula, Alona quadrangularis, Bosmina longirostris, Bosminopsis deitersi, Camptocercus dadayi, Ceriodaphnia richardi, Diaphanosoma fluviatile, Kurzia latissima and Pleuroxus similis. The highest total abundance of Cladocera occurred in April in the marine zone of the estuary. The most abundant species during this period was Penilia avirostris. At the mixing and freshwater zones of the estuary, the most abundant species were Moina micrura, mainly in January; and Simocephalus vetalus and Bosmina longirostris during spring. From this scenario, it can be inferred that the cladoceran community of the Paraíba do Sul River estuary presents characteristics of marine, brackish and freshwater environments. Temperature and salinity seem to limit the occurrence and distribution of cladocerans in the estuary.O presente estudo teve como objetivo caracterizar a comunidade zooplanctônica de cladóceros no estuário do Rio Paraíba do Sul, localizado no município de São João da Barra, RJ. O zooplâncton foi coletado mensalmente de setembro/2002 a agosto/2003 em nove estações através de arrastos subsuperficiais com uma rede de plâncton com abertura de boca de 30 cm e malha de 70 mµ, dotada de fluxômetro mecânico. Foram medidas simultaneamente a

  5. Distribution, abundance and biomass of Chaetognaths off São Sebastião region, Brazil in February 1994

    Directory of Open Access Journals (Sweden)

    Tsui-Hua Liang

    2002-01-01

    Full Text Available The distribution, abundance, biomass, population structure and feeding habits of chaetognaths collected off São Sebastião region, Brazil, in February 1994 are described. Bongo nets were hauled obliquely to collect zooplankton samples. Forty-three samples obtained with the 333 urn mesh were analysed. In this study, 7 chaetognath species belonging to two genera were identified. Sagitta friderici, S. tenuis and S. bipunctata were grouped into the neritic category, and Sagitta enflata, S. hispida, S. minima and Krohnita pacifica into the semi-neritic group. The analysis of the community structure distinguished 3 zones: 1 a nearshore zone evidenced by low richness; 2 an offshore zone evidenced by higher number of species and 3 another offshore zone, located south and south-westward of São Sebastião Island, characterised by higher richness but with dominance of one species. The nearshore zone was dominated by the neritic species S. friderici and S. tenuis, whereas the offshore zone was dominated by S. enflata. Abundance and biomass increase from nearshore toward offshore zones by about two orders of magnitude. Gut content analysis revealed over 90% of empty guts. The chaetognath population was composed mainly of juveniles. The diets among the different chaetognath species was very similar, composed mostly of small copepods and appendicularians.No presente trabalho foram estudados a distribuição, abundância, biomassa, estrutura da população e hábito alimentar dos quetógnatos coletados na região de São Sebastião, Brasil, em fevereiro de 1994. As 43 amostras de zooplâncton utilizadas na elaboração deste trabalho foram obtidas através de arrastos oblíquos usando uma rede Bongô (333 um, providas de fluxômetro. Foram identificadas sete espécies de Chaetognatha pertencentes a dois gêneros. Sagitta friderici, S. tenuis e S. bipunctata foram agrupadas como espécies neríticas, enquanto que Sagitta enflata, S. hispida, S. minima e

  6. Clinical value of human papillomavirus genotype distribution in HPV infection%人乳头瘤病毒亚型在其感染相关疾病中检测的意义

    Institute of Scientific and Technical Information of China (English)

    贾英; 周敏; 吴瑾; 唐良萏

    2009-01-01

    目的 通过分析人乳头瘤病毒(human papiilomavirus,HPV)不同亚型的分布特点,探讨不同的基因型与HPV感染相关疾病的关系.方法 回顾性分析在门诊确诊为宫颈上皮内瘤样病变(cervical intraepithelial neoplasia.CIN)Ⅰ、CIN Ⅱ、CINⅢ、宫颈浸润癌、生殖道湿疣、宫颈HPV亚临床感染共353例患者的HPV哑型的分布情况.结果 353例妇女中HPV阳性176例,阳性率为49.86%.浸润癌中HPV的检出率为100%,且均为高危性HPV感染;HPV感染率在CIN Ⅰ、CIN Ⅱ、CINⅢ、浸润癌逐渐增高,与病变程度密切相关(P<0.05).共检测出15种HPV亚型,依次为16、58、52、33、53、11、31、6、18型等,前4种亚型在宫颈癌和癌前病变中最常见,16、58型等高危型HPV感染在HPV感染相关的良性疾病中仍然最常见,低危型HPV在生殖道湿疣、亚临床感染中却相对少见.结论 HPV检测和分型具有重要意义,HPV感染的型别具有地域差异性;HPV16型的感染仍是女性健康的最大威胁;应重视生殖道湿疣和SPI等良性病变患者的治疗和随访.%Objective To analyze the distribution of human papillomavirus ( HPV) genotype in the disea-ses associated with HPV infection and explore the role of HPV genotype in these diseases. Methods Retro-spective analysis was carried out to compare HPV genotypes of 353 outpatients suffering the diseases associated with HPV infection including cervical caner, cervical intraepithelial neoplasia (CIN) , genital due condyloma, and subclinical cervical human papillomavirus infection (SP1) in our department from January to October 2008. HybriMax were used to detect HPV genotype. The distribution data of HPV genotype were statistically analyzed. Results Totally 176 HPV positive females were found with a positive rate of 49. 86%. HPV positive rate in cervical cancer was 100% , and all of them were high risk HPV. The positive rate was increased with the severi-ty of CIN, and the higher histological

  7. Distribution of HCV genotype and single nucleotide polymorphisms (SNPs of IL-28B gene in HIV/HCV-coinfected Thai populations

    Directory of Open Access Journals (Sweden)

    A Avihingsanon

    2012-11-01

    Full Text Available Introduction: Hepatitis C virus (HCV infection remains a major silent killer, worldwide, particularly in resource poor settings where treatment of hepatitis C is mainly impossible. Pegylated interferon-α (PEG-IFN plus ribavirin (RBV are the recommended treatment for patients with chronic hepatitis C genotype 2/3. Recent study revealed that treatment responses against HCV infection by PEG-IFN and RBV are significantly associated with the single nucleotide polymorphisms (SNPs of interleukin-28B (IL-28B gene. There is limited data about the HCV genotype and SNPs of IL-28B in HIV-infected Thai population. Therefore, we aimed to investigate HCV genotype and the SNP patterns of the IL-28B gene in our HIV/HCV coinfection. Methods: Quantification of HCV RNA was done by a real-time polymerase chain reaction assay (Abbott with lower limit of detection of <12 copies/ml. HCV RNA-positive samples based on reverse transcriptase-polymerase chain reaction (RT-PCR of the 5'UTR were amplified with primer specific for the core and NS5B regions. Nucleotide sequences of both regions were analyzed for the genotype by phylogenetic analysis. DNA sample was extracted from PBMCs or sera. Then SNPs within IL-28B gene were detected by TaqMan real-time PCR (rs8099917 and rs12979860. The data were analyzed by allelic discrimination (AD software on the ABI-7900HT. Results: Totally 60 HIV/HCV-coinfected patients were studied. Median HCV RNA were 5.8 log10 copies/mL, 70% of them had HCV RNA >100,000 copies/mL. After sequencing, the phylogenetic analyses in this study showed that genotype 3 was the most prevalent in this population (56%; following by genotype 1 (30% and 6 (13%. Approximately 4% of them had infected for both genotypes 1 and 3. For IL-28B at rs8099917 and rs12979860 position, 95% of them were major allele (T/T or C/C and 5% were heterozygous (T/G or C/T. Conclusions: HCV genotype 3 is the most prevalent in our HIV/HCV coinfection. 95% of our patients have

  8. Distribution of natural resistance to NS3 protease inhibitors in hepatitis C genotype 1a separated into clades 1 and 2 and in genotype 1b of HIV-infected patients.

    Science.gov (United States)

    Bagaglio, S; Uberti-Foppa, C; Messina, E; Merli, M; Hasson, H; Andolina, A; Galli, A; Lazzarin, A; Morsica, G

    2016-04-01

    Naturally occurring resistance-associated variants (RAVs) within the protease domain of hepatitis C virus (HCV) genotype (G) 1a separated into clades 1 and 2, and G1b were investigated in 59 HIV/HCV coinfected patients. RAVs were detected in 10/23 G1a/clade 1 and 1/19 G1b (p 0.0059). A similar frequency of RAVs was found when comparing G1a/clade 2 and G1b (p 0.1672). A cross-resistance to the macrocyclic compounds simeprevir and paritaprevir was detected in two G1a/clade 2 and 1 G1b sequences and none of G1a/clade 1 sequences. The simultaneous characterization of subtype and natural RAVs by population analysis of the NS3 domain by may add important information for anti-HCV treatment strategies including protease inhibitors.

  9. Aging, Alzheimer’s, and APOE genotype influence the expression and neuronal distribution patterns of microtubule motor protein dynactin-P50

    Directory of Open Access Journals (Sweden)

    Orwa eAboud

    2015-03-01

    Full Text Available Reports from neural cell cultures and experimental animal studies provide evidence of age- and disease-related changes in retrograde transport of spent or misfolded proteins destined for degradation or recycling. However, few studies address these issues in human brain from those who either age without dementia and overt neuropathology, or succumb to Alzheimer’s; especially as such propensity may be influenced by APOE genotype. We studied the expression and distribution of the dynein subunit dynactin-P50, the β amyloid precursor protein (βAPP, and hyperphosphorylated tau (P-tau in tissues and tissue sections of brains from non-demented, neuropathology-free patients and from Alzheimer patients, with either APOE ε3,3 or APOE ε4,4. We found that advanced age in patients without dementia or neuropathological change was associated with coordinated increases in dynactin-P50 and βAPP in neurons in pyramidal layers of the hippocampus. In contrast, in Alzheimer’s, βAPP and dynactin were significantly reduced. Furthermore, the dynactin-P50 and βAPP that was present was located primarily in dystrophic neurites in Aβ plaques. Tissues from Alzheimer patients with APOE ε3,3 had less P-tau, more βAPP, dynactin-P50, and synaptophysin than did tissues from Alzheimer patients carrying APOE ε4,4. It is logical to conclude, then, that as neurons age successfully, there is coordination between retrograde delivery and maintenance and repair, as well as between retrograde delivery and degradation and/or recycling of spent proteins. The buildup of proteins slated for repair, synaptic viability, transport, and re-cycling in neuron soma and dystrophic neurites suggest a loss of this coordination in Alzheimer neurons. Inheritance of APOE ε3,3 rather than APOE ε4,4, is associated with neuronal resilience, suggestive of better repair capabilities, more synapses, more efficient transport, and less hyperphosphorylation of tau. We conclude that even in disease

  10. Distribution, feeding behavior and control strategies of the exotic land snail Achatina fulica (Gastropoda: Pulmonata) in the northeast of Brazil Ecologia do caracol exótico Achatina fulica (Gastropoda:Pulmonata) no nordeste do Brasil

    OpenAIRE

    FS. Albuquerque; MC. Peso-Aguiar; MJT. Assunção-Albuquerque

    2008-01-01

    The goal of this study was to document the distribution and establishment A. fulica such as their feeding preference and behavior in situ. The study was carried out at the city of Lauro de Freitas, Bahia state, Brazil, between November 2001 and November 2002. We used catch per unit effort methods to determine abundance, distribution, habitat choice and food preferences. The abundance and distribution of A. fulica was most representative in urban area, mainly near to the coastline. Lots and ho...

  11. Exploring the diversity and distribution of neotropical avian malaria parasites--a molecular survey from Southeast Brazil.

    Directory of Open Access Journals (Sweden)

    Gustavo A Lacorte

    Full Text Available Southeast Brazil is a neotropical region composed of a mosaic of different tropical habitats and mountain chains, which allowed for the formation of bird-rich communities with distinct ecological niches. Although this region has the potential to harbor a remarkable variety of avian parasites, there is a lack of information about the diversity of malarial parasites. We used molecular approaches to characterize the lineage diversity of Plasmodium and Haemoproteus in bird communities from three different habitats in southeast Brazil based on the prevalence, richness and composition of lineages. We observed an overall prevalence of 35.3%, with a local prevalence ranging from 17.2% to 54.8%. Moreover, no significant association between prevalence and habitat type could be verified (p>0.05. We identified 89 Plasmodium and 22 Haemoproteus lineages, with 86% of them described for the first time here, including an unusual infection of a non-columbiform host by a Haemoproteus (Haemoproteus parasite. The composition analyses of the parasite communities showed that the lineage composition from Brazilian savannah and tropical dry forest was similar, but it was different from the lineage composition of Atlantic rainforest, reflecting the greater likeness of the former habitats with respect to seasonality and forest density. No significant effects of habitat type on lineage richness were observed based on GLM analyses. We also found that sites whose samples had a greater diversity of bird species showed a greater diversity of parasite lineages, providing evidence that areas with high bird richness also have high parasite richness. Our findings point to the importance of the neotropical region (southeast Brazil as a major reservoir of new haemosporidian lineages.

  12. Spatial distribution and risk factors for Toxoplasma gondii seropositivity in cattle slaughtered for human consumption in Rondônia, North region, Brazil.

    Science.gov (United States)

    de Souza, Juliana Bianca Rocha; Soares, Vando Edésio; Maia, Maerle Oliveira; Pereira, Cleidiane Magalhães; Ferraudo, Antônio Sergio; Cruz, Breno Cayeiro; Pires Teixeira, Weslen Fabrício; Felippelli, Gustavo; Maciel, Willian Giquelin; Gonçalves, Walter Antonio; da Costa, Alvimar José; Zanetti Lopes, Welber Daniel

    2016-08-15

    The present study aimed to evaluate Toxoplasma gondii seroprevalence in cattle slaughtered for human consumption from rural properties in the state of Rondônia, North region, Brazil; the seroprevalence was determined using indirect immunofluorescence assays (IFATs). Additionally, spatial distribution and risk factors associated with toxoplasmosis were also analyzed. Of the 1000 cattle serum samples examined, 53 (5.3%) were determined to be seropositive for T. gondii with antibody titers (IgG) ≥64. In regard to results of the studied risk factors (presence of cats, cats with free access to cattle, breeding system, animal's gender, consumption of raw milk by humans on the property and cattle abortion in the last 12 months) and the odds ratio (OR) of each of these factors influencing cattle to acquire toxoplasmosis, only animals raised on a feeder/stocker/backgrounder system presented a higher probability of being seropositive for T. gondii (OR≥1, P=0.04) than cattle raised only in a feeder/stocker system. There was no association between the occurrence of reproductive problems and T. gondii seropositivity. Based on results obtained in the Brazilian state of Rondônia, it could be concluded that the presence of cats and their contact with cattle on each property, cattle breeding purpose and cattle abortion in the last 12 months were not considered risk factors for T. gondii infection in cattle. Considering that the presence of T. gondii was detected in animals slaughtered in the state of Rondônia, consuming raw or undercooked meat from seropositive cattle should be considered a route of transmission of T. gondii to humans. However, the prevalence of toxoplasmosis diagnosed in cattle from this state (5.30%) is lower than the prevalence of toxoplasmosis observed in South, Southeast and Center-West regions of Brazil, which may vary between 48.5% and 71.0%. The low prevalence of toxoplasmosis in cattle is highlighted in Rondônia, which is the sixth largest state

  13. Spatial distribution and risk factors for Toxoplasma gondii seropositivity in cattle slaughtered for human consumption in Rondônia, North region, Brazil.

    Science.gov (United States)

    de Souza, Juliana Bianca Rocha; Soares, Vando Edésio; Maia, Maerle Oliveira; Pereira, Cleidiane Magalhães; Ferraudo, Antônio Sergio; Cruz, Breno Cayeiro; Pires Teixeira, Weslen Fabrício; Felippelli, Gustavo; Maciel, Willian Giquelin; Gonçalves, Walter Antonio; da Costa, Alvimar José; Zanetti Lopes, Welber Daniel

    2016-08-15

    The present study aimed to evaluate Toxoplasma gondii seroprevalence in cattle slaughtered for human consumption from rural properties in the state of Rondônia, North region, Brazil; the seroprevalence was determined using indirect immunofluorescence assays (IFATs). Additionally, spatial distribution and risk factors associated with toxoplasmosis were also analyzed. Of the 1000 cattle serum samples examined, 53 (5.3%) were determined to be seropositive for T. gondii with antibody titers (IgG) ≥64. In regard to results of the studied risk factors (presence of cats, cats with free access to cattle, breeding system, animal's gender, consumption of raw milk by humans on the property and cattle abortion in the last 12 months) and the odds ratio (OR) of each of these factors influencing cattle to acquire toxoplasmosis, only animals raised on a feeder/stocker/backgrounder system presented a higher probability of being seropositive for T. gondii (OR≥1, P=0.04) than cattle raised only in a feeder/stocker system. There was no association between the occurrence of reproductive problems and T. gondii seropositivity. Based on results obtained in the Brazilian state of Rondônia, it could be concluded that the presence of cats and their contact with cattle on each property, cattle breeding purpose and cattle abortion in the last 12 months were not considered risk factors for T. gondii infection in cattle. Considering that the presence of T. gondii was detected in animals slaughtered in the state of Rondônia, consuming raw or undercooked meat from seropositive cattle should be considered a route of transmission of T. gondii to humans. However, the prevalence of toxoplasmosis diagnosed in cattle from this state (5.30%) is lower than the prevalence of toxoplasmosis observed in South, Southeast and Center-West regions of Brazil, which may vary between 48.5% and 71.0%. The low prevalence of toxoplasmosis in cattle is highlighted in Rondônia, which is the sixth largest state

  14. Distribution and Properties of Aerosol and Gas Phase Constituents within Biomass Burning Regional Haze in Brazil, 2012, during the Sambba (South American Biomass Burning Analysis) Field Campaign

    Science.gov (United States)

    Darbyshire, E.; Morgan, W.; Allan, J. D.; Flynn, M.; Liu, D.; O'Shea, S.; Trembath, J.; Szpek, K.; Langridge, J.; Brooke, J.; Ferreira De Brito, J.; Johnson, B. T.; Haywood, J.; Longo, K.; Artaxo, P.; Coe, H.

    2014-12-01

    Biomass Burning (BB) aerosols (BBA) impact upon weather, climate, ecosystems and human health at global and regional scales. Yet quantitative evaluation is impeded by a limited understanding of BB processes and a dearth of in-situ measurements. Thus large model uncertainties prevail, especially in data poor, intensive BB regions such as Brazil. Hence the timely nature of the SAMBBA campaign, utilizing aircraft (UK Facility for Airborne Atmospheric Measurement BAe-146) and ground based observations out of Porto Velho in Sept-Oct 2012. This work utilizes aircraft measurements to characterize BB regional haze - the inhomogeneous accumulation of aged BBA capped within the boundary layer, present across swathes of Brazil. As context, aerosol optical depth (AOD) and meteorological climatologies are presented and compared to the synoptic conditions of 2012. Throughout the early flights an expansive area of elevated (>1) AOD persisted, although in transitioning toward the wet season, rain out and advection significantly reduced its spatial extent and magnitude in western regions of Brazil. Concurrent decreases in haze BBA concentrations (~50%) were observed from the aircraft measurements sampling in these deforested/forested areas. However, the relative vertical structure, composition, physical and optical properties remained similar. The lofted maxima in aerosol concentrations at ~1.5km, typically not captured in models, is potentially important for regional climate. Significant differences were observed, however, during flights over the eastern savannah-like regions of Brazil, which remained drier throughout. Here, haze BBA concentrations resembled those in the west prior to wash out, with the exception of high loadings of refractive black carbon. This acted to lower the single scattering albedo and alter the number size distribution. The observed haze BBA west-east split is also present at source and remains similar throughout fresh plume evolution, thus we conclude

  15. Amphibian-killing chytrid in Brazil comprises both locally endemic and globally expanding populations.

    Science.gov (United States)

    Jenkinson, T S; Betancourt Román, C M; Lambertini, C; Valencia-Aguilar, A; Rodriguez, D; Nunes-de-Almeida, C H L; Ruggeri, J; Belasen, A M; da Silva Leite, D; Zamudio, K R; Longcore, J E; Toledo, F L; James, T Y

    2016-07-01

    Chytridiomycosis, caused by the fungus Batrachochytrium dendrobatidis (Bd), is the emerging infectious disease implicated in recent population declines and extinctions of amphibian species worldwide. Bd strains from regions of disease-associated amphibian decline to date have all belonged to a single, hypervirulent clonal genotype (Bd-GPL). However, earlier studies in the Atlantic Forest of southeastern Brazil detected a novel, putatively enzootic lineage (Bd-Brazil), and indicated hybridization between Bd-GPL and Bd-Brazil. Here, we characterize the spatial distribution and population history of these sympatric lineages in the Brazilian Atlantic Forest. To investigate the genetic structure of Bd in this region, we collected and genotyped Bd strains along a 2400-km transect of the Atlantic Forest. Bd-Brazil genotypes were restricted to a narrow geographic range in the southern Atlantic Forest, while Bd-GPL strains were widespread and largely geographically unstructured. Bd population genetics in this region support the hypothesis that the recently discovered Brazilian lineage is enzootic in the Atlantic Forest of Brazil and that Bd-GPL is a more recently expanded invasive. We collected additional hybrid isolates that demonstrate the recurrence of hybridization between panzootic and enzootic lineages, thereby confirming the existence of a hybrid zone in the Serra da Graciosa mountain range of Paraná State. Our field observations suggest that Bd-GPL may be more infective towards native Brazilian amphibians, and potentially more effective at dispersing across a fragmented landscape. We also provide further evidence of pathogen translocations mediated by the Brazilian ranaculture industry with implications for regulations and policies on global amphibian trade. PMID:26939017

  16. Description of Microvelia urucara sp. nov. and new distributional data on veliids (Insecta: Heteroptera: Veliidae: from the Amazon River floodplain, Brazil

    Directory of Open Access Journals (Sweden)

    Felipe F. F Moreira

    2011-10-01

    Full Text Available Based on material collected on streams and lakes from the Amazon River floodplain, Brazil, Microvelia urucara sp. nov. is described, illustrated and compared with similar species. The new species, like many other Neotropical Microvelia Westwood, 1834, does not present striking modifications on the body or appendages, but can be separated from its congeners by features of the male genitalia. Distributional data is presented for other veliids collected along the Amazon River, and Paravelia capixaba Moreira, Nessimian & Rúdio, 2010 and Microvelia summersi Drake & Harris, 1928 are recorded for the first time from the Brazilian Amazon. Rhagovelia jubata Bacon, 1948 is newly recorded from the state of Amazonas, and Microvelia mimula White, 1879, M. pulchella Westwood, 1834 and M. venustatis Drake & Harris, 1933 are recorded for the first time from the state of Pará.

  17. An analysis of the spatiotemporal distribution of American cutaneous leishmaniasis in counties located along road and railway corridors in the State of Maranhao, Brazil

    Directory of Open Access Journals (Sweden)

    Vicente Silva Goncalves Neto

    2013-06-01

    Full Text Available Introduction The incidence of American cutaneous leishmaniasis (ACL is increasing in Latin America, especially in Brazil, where 256,587 cases were confirmed in the last decade. Methods This study used a Bayesian model to examine the spatial and temporal distribution of ACL cases between 2000 and 2009 in 61 counties of State of Maranhão located along the three main road and railway corridors. Results During the study period, 13,818 cases of ACL were recorded. There was a significant decrease in the incidence of ACL in the ten study years. The recorded incidence rate ranged from 7.36 to 241.45 per 100,000 inhabitants. The relative risk increased in 77% of the counties, decreased in 18% and was maintained in only five counties. Conclusions Although there was a decreased incidence of the disease, ACL was present in all of the examined municipalities, thus maintaining the risk of contracting this illness.

  18. Streptococcus suis in employees and the environment of swine slaughterhouses in São Paulo, Brazil: Occurrence, risk factors, serotype distribution, and antimicrobial susceptibility.

    Science.gov (United States)

    Soares, Taíssa Cook Siqueira; Gottschalk, Marcelo; Lacouture, Sonia; Megid, Jane; Ribolla, Paulo Eduardo Martins; Pantoja, José Carlos de Figueiredo; Paes, Antonio Carlos

    2015-10-01

    Streptococcus suis is an important pathogen in the swine industry. This article is the first to report the occurrence, risk factors, serotype distribution, and antimicrobial susceptibility of S. suis recovered from employees and environmental samples of swine slaughterhouses in Brazil. Tonsillar swabs from all 139 pig-slaughtering employees and 261 environmental swabs were collected for detection of S. suis and serotyping by monoplex and multiplex polymerase chain reaction, respectively. Antimicrobial susceptibility was determined by the disk-diffusion method. Although S. suis was not detected in any of the tested employees, it was isolated from 25% of the environmental samples. Significant differences (P cephalexin (75%) were observed. However, multidrug resistance was observed in all the isolates. Because S. suis is present in the environment of swine slaughterhouses, on carcasses and knives, as well as on the hands of employees in all areas, all employees are at risk of infection. PMID:26424907

  19. Selection of Cactus Pear Forage (Opuntia spp. and (Nopalea spp. Genotypes resistant to the Carmine Cochineal (Dactylopius opuntiae cockerell, 1929 in the State of Paraíba, Brazil

    Directory of Open Access Journals (Sweden)

    Edson Batista Lopes

    2010-04-01

    Full Text Available Since 2001, the giant cactus pear (O. ficus-indica has been decimated by carmine cochineal (Dactylopius opuntiae Cockerell, 1929, considered a potentially devastating pest. The objective of this research was to select genotypes resistant of cactus pear to the carmine cochineal. The experiment was conducted at the Laboratory of Plant Protection of Lagoa Seca Experimental Station owned by the Agricultural Research Corporation of Paraiba-EMEPA-PB, during the months of February to July 2009. We evaluated 22 genotypes of cactus pear (Opuntia spp. and Nopalea spp.. Of each genotype were selected five cladodes, standardized in relation to the physiological state (age of the cladodes. They were divided into four quadrants, where one in every five of them were applied “toothpicks” infested with the insect colonies, a total of 20 colonies/cladodes. Weekly cladodes of the infested genotypes were inspected to evaluate the growth of old colonies and the emergence of new colonies. This procedure took 60 days. The results showed that the genotypes Palma ornamental (Opuntia stricta,, X-Italiana, Palma Gigante, F5, F8, F11, V12, IPA-Clone 20, Orelha-de-onça (Opuntia sp., Redonda, Branco São Pedro, Formosa, Lingua-de-vaca and Gigantona are susceptible to the carmine cochineal. Already the genotypes Baiana or Alagoana, Palma Doce or Miúda Orelha-de-elefante-africana, Orelha-de-elefante-mexicana, Orelha-de-onça (Nopalea sp. and Palma Azul are resistant to the carmine cochineal.

  20. Seis novas larvas do gênero Micrathyria Kirby e notas sobre a distribuição no Brasil (Odonata, Libellulidae Six new larvae of genus Micrathyria Kirby and notes on distribution in Brazil. (Odonata, Libellulidae

    Directory of Open Access Journals (Sweden)

    Cristiane Vieira de Assis

    1994-01-01

    Full Text Available Six new Micrathyria Kirby, 1889 larvae from Brazil are described and compared to those described by SANTOS (1968, 1972, 1978 and NEEDHAM (1943. The distribution of the species is studied based on material deposited in colection of Museu Nacional, Universidade Federal do Rio de Janeiro (MN-UFRJ.

  1. Spatial variations of environmental tracer distributions in water from a mangrove ecosystem: The case of Babitonga Bay (Santa Catarina, Brazil)

    International Nuclear Information System (INIS)

    The hydrologic complex of Babitonga Bay (Brazil) forms a vast environmental complex, where agriculture, shellfish farming, and industries coexist with a unique natural area of Atlantic rain forest. The origin of different continental hydrological components, the environmental transition between saline and fresh waters, and the influence of the seasonality on Babitonga Bay waters are evaluated using isotopes and chemistry. The end of the dry season is marked by a fast response of continental water to the first rainfall, while in the Bay this change is delayed in time. At the end of the rainy season waters show a more homogeneous isotopic composition, suggesting the harmonisation of hydrological and hydrogeological systems. Moreover, δ13C and δ15N of DIC and POM allows for the definition of the biogeochemical processes originating and transporting chemical compounds in the coastal and transition areas and for the determination of three distinct end-members: terrestrial, marine and urban. (author)

  2. Spatial variations of environmental tracers distribution in water from a mangrove ecosystem: the case of Babitonga Bay (Santa Catarina, Brazil)

    International Nuclear Information System (INIS)

    The hydrologic complex of Babitonga Bay (Brazil) forms a vast environmental complex, where agriculture, shellfish farming, and industries coexist with a unique natural area of Atlantic rain forest. The origin of different continental hydrological components, the environmental transition between saline and fresh waters, and the influence of the seasonality on Babitonga Bay waters are evaluated using isotopes and chemistry. The end of the dry season is marked by a fast response of continental water to the first rainfall, while in the Bay this change is delayed in time. At the end of the rainy season waters show a more homogeneous isotopic composition, suggesting the harmonisation of hydrological and hydrogeological systems. Moreover δ13C and δ15N of DIC, and POM allows for the definition of the biogeochemical processes originating and transporting chemical compounds in the coastal and transition areas and for the determination of three distinct end-members: terrestrial, marine and urban. (author)

  3. Diesel oil safety stock in storage and distribution terminals: a case study: Porto Velho/Rio, Brazil

    Energy Technology Data Exchange (ETDEWEB)

    Freitas, Rubens C. [Agencia Nacional do Petroleo, Gas Natural e Biocombustiveis (ANP), Rio de Janeiro, RJ (Brazil); D' agosto, Marcio A. [Coordenacao dos Programas de Pos-Graduacao de Engenharia (COPPE/UFRJ), RJ (Brazil)

    2012-07-01

    This paper presents a methodology for calculating diesel oil safety stock in distributor terminals located in the northern of Brazil, applied on a case study for terminals in the state of Rondonia. From the mapping of logistic flows related to transport and storage of diesel oil, the risk factors that cause uncertainties in lead time were analyzed and quantified. Through a research conducted among economic agents (producers, distributors and carriers) operating in the region, it was measured the relevance and impact of risk factors on the safety stock in the terminals. The safety stock was calculated by the classical equation of inventories, as well as by the methodology proposed in this paper. The difference between the two methods has unveiled inefficiencies derived from uncertainties in lead time. Recommendations of actions to mitigate the risk factors are included at the end of this paper. (author)

  4. Distribution of human immunodeficiency virus type 1 subtypes in the State of Amazonas, Brazil, and subtype C identification

    Energy Technology Data Exchange (ETDEWEB)

    Cunha, L.K.H. [Departamento de Parasitologia, Universidade Federal do Amazonas, Manaus, AM (Brazil); Kashima, S.; Amarante, M.F.C.; Haddad, R.; Rodrigues, E.S. [Laboratório de Biologia Molecular, Hemocentro de Ribeirão Preto, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, SP (Brazil); Silva, K.L.T.; Lima, T.A.; Castro, D.B.; Brito, F.C.; Almeida, E.G. [Diretoria de Ensino e Pesquisa,Fundação de Hematologia e Hemoterapia do Amazonas, Manaus, AM (Brazil); Covas, D.T. [Laboratório de Biologia Molecular, Hemocentro de Ribeirão Preto, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, SP (Brazil); Malheiro, A. [Departamento de Parasitologia, Universidade Federal do Amazonas, Manaus, AM (Brazil); Diretoria de Ensino e Pesquisa,Fundação de Hematologia e Hemoterapia do Amazonas, Manaus, AM (Brazil)

    2012-01-20

    Few studies have reported the molecular epidemiological characterization of HIV-1 in the Northern region of Brazil. The present study reports the molecular and epidemiological characterization of 31 HIV-1 isolates from blood donors from the State of Amazonas who donated blood between April 2006 and March 2007. Serum/plasma samples from all donors were screened for HIV antibodies by ELISA and the results confirmed by Western blot analysis. Genomic DNA was extracted from the buffy coat using the Super Quik-Gene-DNA Isolation kit. Nested PCR was performed on the env, gag, and pol regions of HIV-1 using the Gene Amp PCR System 9700. Sequencing reactions were performed using the inner PCR primers and the DYEnamic™ ET Dye Terminator Kit, and phylogenetic analysis was performed using the gag, pol, and env gene sequences. We collected samples from 31 blood donors who tested positive for HIV-1 in confirmatory experiments. The male:female ratio of blood donors was 3.4:1, and the mean age was 32.4 years (range: 19 to 61 years). Phylogenetic analysis showed that subtype B is the most prevalent among Northern Brazilian HIV-1-seropositive blood donors. One HIV-1 subtype C and one circulating recombinant form (CRF-BF) of HIV-1 were identified in the State of Amazonas. This is the first study showing the occurrence of a possible “homogenous” subtype C in this region of Brazil. This finding could contribute to a better characterization of the HIV-1 strains that circulate in the country. Key words: HIV-1; Subtypes; Phylogenetic analysis; Blood donors; Molecular and epidemiological characterization.

  5. Distribution of human immunodeficiency virus type 1 subtypes in the State of Amazonas, Brazil, and subtype C identification

    International Nuclear Information System (INIS)

    Few studies have reported the molecular epidemiological characterization of HIV-1 in the Northern region of Brazil. The present study reports the molecular and epidemiological characterization of 31 HIV-1 isolates from blood donors from the State of Amazonas who donated blood between April 2006 and March 2007. Serum/plasma samples from all donors were screened for HIV antibodies by ELISA and the results confirmed by Western blot analysis. Genomic DNA was extracted from the buffy coat using the Super Quik-Gene-DNA Isolation kit. Nested PCR was performed on the env, gag, and pol regions of HIV-1 using the Gene Amp PCR System 9700. Sequencing reactions were performed using the inner PCR primers and the DYEnamic™ ET Dye Terminator Kit, and phylogenetic analysis was performed using the gag, pol, and env gene sequences. We collected samples from 31 blood donors who tested positive for HIV-1 in confirmatory experiments. The male:female ratio of blood donors was 3.4:1, and the mean age was 32.4 years (range: 19 to 61 years). Phylogenetic analysis showed that subtype B is the most prevalent among Northern Brazilian HIV-1-seropositive blood donors. One HIV-1 subtype C and one circulating recombinant form (CRF-BF) of HIV-1 were identified in the State of Amazonas. This is the first study showing the occurrence of a possible “homogenous” subtype C in this region of Brazil. This finding could contribute to a better characterization of the HIV-1 strains that circulate in the country. Key words: HIV-1; Subtypes; Phylogenetic analysis; Blood donors; Molecular and epidemiological characterization

  6. Study of the distribution characteristics of rare earth elements in Solanum lycocarpum from different tropical environments in Brazil by neutron activation analysis

    International Nuclear Information System (INIS)

    In this work, the concentration of eight rare earth elements (REE), La, Ce, Nd, Sm, Eu, Tb, Yb and Lu, was determined by neutron activation analysis (INAA), in plant leaves of Solanum lycocarpum. This species is a typical Brazilian 'cerrado' plant, widely distributed in Brazil. The analysis of the plant reference materials CRM Pine Needles (NIST 1575) and Spruce Needles (BCR 101) proved that the methodology applied was sufficiently accurate and precise for the determination of REE in plants. In order to better evaluate the uptake of the REE from the soil to the plant, the host soil was also analyzed by ESiAA. The studied areas were Salitre, MG, Serra do Cipo, MG, Lagoa da Pampulha and Mangabeiras, in Belo Horizonte, MG, and Cerrado de Emas, in Pirassununga, SP. The results were analyzed through the calculation of transfer factors soil-plant and by using diagrams normalized to chondrites. The data obtained showed different transfer factors from soil to plant as the subtract changes. Similar distribution patterns for the soil and the plant were obtained in all the studied sites, presenting an enrichment of the light REE (La to Sm), in contrast to the heavy REE (Eu to Lu), less absorbed. These results indicate that the light REE remain available to the plant in the more superficial soil layers. The similarity between the distribution patterns indicates a typical REE absorption by this species, in spite of the significant differences in the substratum . (author)

  7. DISTRIBUTION OF TREE AND SHRUBS SPECIES ALONG OF THE SOIL HUMIDITY GRADIENT FROM PUNCTUAL HEADWATERS OF THE SANTA CRUZ STREAM WATERSHED, LAVRAS, SE BRAZIL

    Directory of Open Access Journals (Sweden)

    Lilian Vilela Andrade Pinto

    2005-09-01

    Full Text Available The objective of this research was to verify the distribution of the tree and shrubs species along the soil humiditygradient in the punctual headwaters environment localized at the Santa Cruz stream watershed, Lavras, Minas Gerais State, Brazil.To understanding the vegetation it was performed a structural survey of shrubs and trees stratum of three degraded and disturbedheadwaters. In each sampled headwaters it was instaled 4 plots of 50 x10m located above (P1, below (P2, on the right (P3 and onthe left (P4 of the headwaters. The area around of the headwaters was separated in two gradients of species. The first gradientconsisted in species present in the plot P2, following the river bed and in the firsts 10m length of the plots P1, P3 and P4. The secondgradient comprised of species present in the plots P1, P3 and P4 from of the 10m length of each plot. In the structural surveys they wererecorded 1.217 individuals distributed in 110 species, 80 genus and 43 botanical families. It was concluded that the exclusiveness ofthe species in the distinct gradients, the value of density of the contrasty individuals for the species in the gradients and the model ofspatial distribution of the species along the river bed demonstrated the specificity of the environment to the headwaters. The specificitydemands different strategies for conservation and reclamation of the headwaters.

  8. Prenatal pesticide exposure and PON1 genotype associated with adolescent body fat distribution evaluated by dual X-ray absorptiometry (DXA)

    DEFF Research Database (Denmark)

    Tinggaard, J; Wohlfahrt-Veje, C; Husby, S;

    2016-01-01

    ) at age 10-15. Prenatal pesticide exposure was associated with increased total, android, and gynoid fat% (DXA) at age 10-15 years after adjustment for sex, socioeconomic status, and puberty (all β = 0.5 standard deviation score (SDS) p ... (total fat: β = 0.7 SDS, android-gynoid ratio: β = 0.1, both p ... circumference were found. Prenatal pesticide exposure was associated with higher adolescent body fat content, including android fat deposition, independent of puberty. Girls appeared more susceptible than boys. Furthermore, the association depended on maternal and child PON1 Q192R genotype....

  9. Prevalence and genotype distribution of cervical human papillomavirus infection among women in Nanjing%南京地区妇女宫颈人乳头瘤病毒感染和亚型分布情况

    Institute of Scientific and Technical Information of China (English)

    刘邺; 成建

    2013-01-01

    Objective;To analyze the prevalence and genotype distribution of cervical human papillomavirus (HPV) infection among women in Nanjing,China. Methods:Fast nucleic acid hybridization chip was performed to detect cervical HPV DNA and genotype of 7 531 women and the prevalence and genotype distribution were analyzed. Results:The overall prevalence of cervical HPV infection was 33.0% among women in Nanjing (age-standardized prevalence was 37.3%). Among 2 485 HPV-positive women,the overall prevalence of HPV infection was highest in the group aged ≤24 years (43.3% )and there was significant difference between the prevalence of different age-stratified group; the overall prevalence of multiple genotypes HPV infection was highest in the group aged ≤24 years(40.0%)and there was significant difference between the prevalence of different age-stratified group;among the detected 21 HPV genotypes,the most common high-risk HPV genotypes were HPV16(8.6%) ,HPV58 (7.6%) ,HPV52(5.2%). Conclusion;The prevalence of cervical human papillomavirus among women aged ≤24 years in Nanjing was relatively high, cervical disease prevention program should give priority to this population;HPV 16,HPV 58 and HPV 52 were the most prevalent cervical HPV genotypes among women in Nanjing.%目的:了解南京地区妇女宫颈人乳头瘤病毒(human papillomavirus,HPV)感染及亚型分布情况.方法:用核酸分子芯片快速杂交法对7 531名妇女进行宫颈脱落细胞HPV DNA基因分型检测,并对HPV感染率、亚型分布进行统计分析.结果:南京地区妇女宫颈部位HPV感染率为33.0%(年龄标准化率为37.3%);在2 485例HPV感染者中,≤24岁年龄组妇女感染率最高,为43.3%,各年龄组间HPV感染率有显著性差异;≤24岁年龄组妇女多重亚型感染率最高,为40.0%,各年龄组间的多重亚型感染率有显著性差异;在所检测出来的21种亚型中,HPV16、58、52是最常见的高危亚型,感染率分别为8.6%、7.6

  10. Genetic Marker Analysis of a Global Collection of Isolates of Citrus tristeza virus: Characterization and Distribution of CTV Genotypes and Association with Symptoms.

    Science.gov (United States)

    Hilf, Mark E; Mavrodieva, Vessela A; Garnsey, Stephen M

    2005-08-01

    ABSTRACT Genetic markers amplified from three noncontiguous regions by sequence specific primers designed from the partial or complete genome sequences of Citrus tristeza virus (CTV) isolates T3, T30, T36, and VT were used to assess genetic relatedness of 372 isolates in an international collection. Eighty-five isolates were judged similar to the T3 isolate, 81 to T30, 11 to T36, and 89 to VT. Fifty-one isolates were mixed infections by two or more identifiable viral genotypes, and 55 isolates could not be assigned unequivocally to a group defined by marker patterns. Maximum parsimony analysis of aligned marker sequences supported the grouping of isolates on the basis of marker patterns only. Specific disease symptoms induced in select citrus host plants were shared across molecular groups, although symptoms were least severe among isolates grouped by markers with the T30 isolate and were most severe among isolates grouped by markers with the T3 isolate. Isolates assigned the same genotype showed variable symptoms and symptom severity. A classification strategy for CTV isolates is proposed that combines genetic marker patterns and nucleotide sequence data. PMID:18944413

  11. Distribution of rare earths, thorium and uranium in bryophytes and soils in Tripui Ecological Station, Minas Gerais, Brazil

    Energy Technology Data Exchange (ETDEWEB)

    Palmieri, Helena E.L. [Centro de Desenvolvimento da Tecnologia Nuclear (CDTN/CNEN-MG), Belo Horizonte, MG (Brazil)], E-mail: help@cdtn.br; Nalini Junior, Herminio A. [Universidade Federal de Ouro Preto (UFOP), MG (Brazil). Dept. de Geologia], E-mail: nalini@degeo.ufop.br; Friese, Kurt [Helmholtz Centre for Environmental Research- UFZ, Magdeburg (Germany). Dept. of Lake Research], E-mail: kurt.friese@ufz.de

    2007-07-01

    The concentrations of rare earth elements (REEs), thorium and uranium were determined in liverworts (Noteroclada confluens (Tayl.) and Dumortirea hirsute (Sw.) Nees), in mosses (Leucobryum martianum (Hornsh.) Hampe, Vesicularia vesicularis (Schwaegr.) Broth., Pyrrhobruym spiniforme (Hedw.) Mitt. and Sematophyllum subsimplex (Hedw.) Mitt.) and in the soil upon which they were growing. The samples were collected on the margins of the main streams of the Tripui Ecological Station, located in the valley of the Tripui stream near the town of Ouro Preto, Minas Gerais State, Brazil. For decades, this Station has been the object of interest of many studies due to its historical, ecological and environmental importance. Analyses in bryophytes (mosses and liverworts) were determined by inductively coupled plasma mass spectrometry (ICP-MS) and in the soil samples by using neutron activation analysis (NAA), specifically the k{sub 0}-standardization method and the energy dispersive spectrometry technique (EDS). Lanthanum (La), cerium (Ce) and neodymium (Nd) were present in higher concentrations in soils and bryophytes than other REEs. It was observed that in all the collected bryophytes species the elements Th, U, Y, La, Ce, Pr, Nd, Sm, Eu, Gd, Tb, Dy, Ho, Er and Yb were transferred from the soil water to these plants. These bryophytes presented different capacities of accumulating these elements with the liverworts (Noteroclada confluens and Dumortirea hirsute) and the moss Leucobryum martianum showing a more efficient accumulation capacity than the other bryophyte species. (author)

  12. Distribution of strain type and antimicrobial susceptibility of Escherichia coli isolates causing meningitis in a large urban setting in Brazil.

    Science.gov (United States)

    Berman, Hillary; Barberino, Maria Goreth; Moreira, Edson Duarte; Riley, Lee; Reis, Joice N

    2014-05-01

    The clinical management of meningitis caused by Escherichia coli is greatly complicated when the organism becomes resistant to broad-spectrum antibiotics. We sought to characterize the antimicrobial susceptibilities, sequence types (ST), and presence of known drug resistance genes of E. coli isolates that caused meningitis between 1996 and 2011 in Salvador, Brazil. We then compared these findings to those for E. coli isolates from community-acquired urinary tract infections (UTI) that occurred during the same time period and in the same city. We found that 19% of E. coli isolates from cases of meningitis and less than 1% of isolates from UTI were resistant to third-generation cephalosporins. The sequence types of E. coli isolates from cases of meningitis included ST131, ST69, ST405, and ST62, which were also found among isolates from UTI. Additionally, among the E. coli isolates that were resistant to third-generation cephalosporins, we found genes that encode the extended-spectrum beta-lactamases CTX-M-2, CTX-M-14, and CTX-M-15. These observations demonstrate that compared to E. coli strains isolated from cases of community-acquired UTI, those isolated from cases of meningitis are more resistant to third-generation cephalosporins, even though the same sequence types are shared between the two forms of extraintestinal infections.

  13. Distribution of rare earths, thorium and uranium in bryophytes and soils in Tripui Ecological Station, Minas Gerais, Brazil

    International Nuclear Information System (INIS)

    The concentrations of rare earth elements (REEs), thorium and uranium were determined in liverworts (Noteroclada confluens (Tayl.) and Dumortirea hirsute (Sw.) Nees), in mosses (Leucobryum martianum (Hornsh.) Hampe, Vesicularia vesicularis (Schwaegr.) Broth., Pyrrhobruym spiniforme (Hedw.) Mitt. and Sematophyllum subsimplex (Hedw.) Mitt.) and in the soil upon which they were growing. The samples were collected on the margins of the main streams of the Tripui Ecological Station, located in the valley of the Tripui stream near the town of Ouro Preto, Minas Gerais State, Brazil. For decades, this Station has been the object of interest of many studies due to its historical, ecological and environmental importance. Analyses in bryophytes (mosses and liverworts) were determined by inductively coupled plasma mass spectrometry (ICP-MS) and in the soil samples by using neutron activation analysis (NAA), specifically the k0-standardization method and the energy dispersive spectrometry technique (EDS). Lanthanum (La), cerium (Ce) and neodymium (Nd) were present in higher concentrations in soils and bryophytes than other REEs. It was observed that in all the collected bryophytes species the elements Th, U, Y, La, Ce, Pr, Nd, Sm, Eu, Gd, Tb, Dy, Ho, Er and Yb were transferred from the soil water to these plants. These bryophytes presented different capacities of accumulating these elements with the liverworts (Noteroclada confluens and Dumortirea hirsute) and the moss Leucobryum martianum showing a more efficient accumulation capacity than the other bryophyte species. (author)

  14. Hepatitis C virus genotypes in Myanmar

    Science.gov (United States)

    Win, Nan Nwe; Kanda, Tatsuo; Nakamoto, Shingo; Yokosuka, Osamu; Shirasawa, Hiroshi

    2016-01-01

    Myanmar is adjacent to India, Bangladesh, Thailand, Laos and China. In Myanmar, the prevalence of hepatitis C virus (HCV) infection is 2%, and HCV infection accounts for 25% of hepatocellular carcinoma. In this study, we reviewed the prevalence of HCV genotypes in Myanmar. HCV genotypes 1, 3 and 6 were observed in volunteer blood donors in and around the Myanmar city of Yangon. Although there are several reports of HCV genotype 6 and its variants in Myanmar, the distribution of the HCV genotypes has not been well documented in areas other than Yangon. Previous studies showed that treatment with peginterferon and a weight-based dose of ribavirin for 24 or 48 wk could lead to an 80%-100% sustained virological response (SVR) rates in Myanmar. Current interferon-free treatments could lead to higher SVR rates (90%-95%) in patients infected with almost all HCV genotypes other than HCV genotype 3. In an era of heavy reliance on direct-acting antivirals against HCV, there is an increasing need to measure HCV genotypes, and this need will also increase specifically in Myanmar. Current available information of HCV genotypes were mostly from Yangon and other countries than Myanmar. The prevalence of HCV genotypes in Myanmar should be determined.

  15. Soils distribution model based on relation between geology, geomorphology and pedology, at the High Plateau of Distrito Federal, Brazil

    OpenAIRE

    Inara Oliveira Barbosa; Marilusa Pinto Coelho Lacerda; Marina Rolim Bilich

    2010-01-01

    Studies of the relation between geology and geomorphic surfaces are important for understanding the soil distribution in the landscape. In the Distrito Federal the scale survey does not evidence some pedologic features. This study aimed to generate a pedological distribution model in Distrito Federal High Plateau with more detailed scale, from the relations between geology, geomorphology and soils. The study area is located in the midwest portion of the Distrito Federal. To generate the soil ...

  16. Recent advances in the study of avian malaria: an overview with an emphasis on the distribution of Plasmodium spp in Brazil

    Directory of Open Access Journals (Sweden)

    Érika Martins Braga

    2011-08-01

    Full Text Available Avian malaria parasites (Plasmodium have a worldwide distribution except for Antarctica. They are transmitted exclusively by mosquito vectors (Diptera: Culicidae and are of particular interest to health care research due to their phylogenetic relationship with human plasmodia and their ability to cause avian malaria, which is frequently lethal in non-adapted avian hosts. However, different features of avian Plasmodium spp, including their taxonomy and aspects of their life-history traits, need to be examined in more detail. Over the last 10 years, ecologists, evolutionary biologists and wildlife researchers have recognized the importance of studying avian malaria parasites and other related haemosporidians, which are the largest group of the order Haemosporida by number of species. These studies have included understanding the ecological, behavioral and evolutionary aspects that arise in this wildlife host-parasite system. Molecular tools have provided new and exiting opportunities for such research. This review discusses several emerging topics related to the current research of avian Plasmodium spp and some related avian haemosporidians. We also summarize some important discoveries in this field and emphasize the value of using both polymerase chain reaction-based and microscopy-based methods in parallel for wildlife studies. We will focus on the genus Plasmodium, with an emphasis on the distribution and pathogenicity of these parasites in wild birds in Brazil.

  17. Influence of Topographic and Hydrographic Factors on the Spatial Distribution of Leptospirosis Disease in São Paulo County, Brazil: An Approach Using Geospatial Techniques and GIS Analysis

    Science.gov (United States)

    Ferreira, M. C.; Ferreira, M. F. M.

    2016-06-01

    Leptospirosis is a zoonosis caused by Leptospira genus bacteria. Rodents, especially Rattus norvegicus, are the most frequent hosts of this microorganism in the cities. The human transmission occurs by contact with urine, blood or tissues of the rodent and contacting water or mud contaminated by rodent urine. Spatial patterns of concentration of leptospirosis are related to the multiple environmental and socioeconomic factors, like housing near flooding areas, domestic garbage disposal sites and high-density of peoples living in slums located near river channels. We used geospatial techniques and geographical information system (GIS) to analysing spatial relationship between the distribution of leptospirosis cases and distance from rivers, river density in the census sector and terrain slope factors, in Sao Paulo County, Brazil. To test this methodology we used a sample of 183 geocoded leptospirosis cases confirmed in 2007, ASTER GDEM2 data, hydrography and census sectors shapefiles. Our results showed that GIS and geospatial analysis techniques improved the mapping of the disease and permitted identify the spatial pattern of association between location of cases and spatial distribution of the environmental variables analyzed. This study showed also that leptospirosis cases might be more related to the census sectors located on higher river density areas and households situated at shorter distances from rivers. In the other hand, it was not possible to assert that slope terrain contributes significantly to the location of leptospirosis cases.

  18. Biomphalaria species distribution and its effect on human Schistosoma mansoni infection in an irrigated area used for rice cultivation in northeast Brazil

    Directory of Open Access Journals (Sweden)

    Delmany Moitinho Barboza

    2012-09-01

    Full Text Available The role of irrigated areas for the spread of schistosomiasis is of worldwide concern. The aim of the present study was to investigate the spatial distribution of the intermediate snail host Biomphalaria in an area highly endemic for schistosomiasis due to Schistosoma mansoni, evaluating the relationship between irrigation and types of natural water sources on one hand, and the influence of place and time of water exposure on the intensity of human infection on the other. A geographical information system (GIS was used to map the distribution of the intermediate snail hosts in Ilha das Flores, Sergipe, Brazil, combined with a clinical/epidemiological survey. We observed a direct correlation between the intensity of human infection with S. mansoni and irrigation projects. Malacological studies to identify snail species and infection rates showed that B. glabrata is the main species responsible for human schistosomiasis in the municipality, but that B. straminea also plays a role. Our results provide evidence for a competitive selection between the two snail species in rice fields with a predominance of B. glabrata in irrigation systems and B. straminea in natural water sources.

  19. Population biology and distribution of the portunid crab Callinectes ornatus (Decapoda: Brachyura in an estuary-bay complex of southern Brazil

    Directory of Open Access Journals (Sweden)

    Timoteo T. Watanabe

    2014-08-01

    Full Text Available Trawl fisheries are associated with catches of swimming crabs, which are an important economic resource for commercial as well for small-scale fisheries. This study evaluated the population biology and distribution of the swimming crab Callinectes ornatus (Ordway, 1863 in the Estuary-Bay of São Vicente, state of São Paulo, Brazil. Crabs were collected from a shrimp fishing boat equipped with a semi-balloon otter-trawl net, on eight transects (four in the estuary and four in the bay from March 2007 through February 2008. Specimens caught were identified, sexed and measured. Samples of bottom water were collected and the temperature and salinity measured. A total of 618 crabs were captured (332 males, 267 females and 19 ovigerous females, with a sex ratio close to 1:1. A large number of juveniles were captured (77.67%. Crab spatial distributions were positively correlated with salinity (Rs = 0.73, p = 0.0395 and temperature (Rs = 0.71, p = 0.0092. Two peaks of recruitment occurred, in summer and autumn, and ovigerous females were mostly captured during summer, showing a seasonal reproductive pattern. The results showed that C. ornatus uses the bay as a nursery area for juvenile development. Callinectes ornatus is not yet a legally protected species, and the minimum allowed size of crabs caught in the area, although already restricted, should be carefully evaluated since the removal of large numbers of juveniles could negatively impact the local population.

  20. Spatial distribution and concentration assessment of total petroleum hydrocarbons in the intertidal zone surface sediment of Todos os Santos Bay, Brazil.

    Science.gov (United States)

    Silva, Carine S; Moreira, Icaro T A; de Oliveira, Olivia M C; Queiroz, Antonio F S; Garcia, Karina S; Falcão, Brunno A; Escobar, Narayana F C; Rios, Mariana Cruz

    2014-02-01

    The primary objective of this study was to investigate the concentrations and spatial distribution of the total petroleum hydrocarbons (TPHs) in the intertidal zone surface sediment of Todos os Santos Bay, Brazil, to assess the distribution and degree of contamination by TPHs, measure the level of TPH degradation in the surface sediment, and identify the organic matter sources. The surface sediment used in this study was collected in 50 stations, and TPHs, isoprenoid alkanes (pristane and phytane), and unresolved complex mixture (UCM) were analyzed by gas chromatography with a flame ionization detector. The total concentrations ranged from 0.22 to 40,101 μg g(-1) dry weight and showed a strong correlation with the total organic carbon (TOC) content. The highest TPH concentrations were observed in samples from the mangrove sediments of a river located near a petroleum refinery. Compared with other studies in the world, the TPH concentrations in the intertidal surface sediment of Todos os Santos Bay were below average in certain stations and above average in others. An analysis of the magnitude of UCM (0.11 to 17,323 μg g(-1) dry weight) and the ratios nC17/Pr and nC18/Ph suggest that an advanced state of oil weathering, which indicates previous contamination. The molar C/N ratios varied between 5 and 43, which indicate organic matter with a mixed origin comprising marine and continental contributions. PMID:24100798

  1. Study on the Genotype Distributions of Human Papillomavirus Infection in Women of Tianjin%天津市妇女人乳头瘤病毒感染各亚型分布的研究

    Institute of Scientific and Technical Information of China (English)

    黎科; 李琳

    2015-01-01

    目的:调查天津市妇女宫颈人乳头瘤病毒(HPV)感染现状和基因各亚型分布情况,为研究宫颈癌疫苗和防治宫颈癌提供依据。方法采用聚合酶链式反应(Polymerase Chain Reaction,PCR)反向点杂交基因芯片技术对6132名做宫颈癌筛查的女性宫颈脱落细胞标本进行HPV基因分型检测,分析HPV在女性人群中的感染率、基因型别和年龄分布特点。结果6132例标本中HPV阳性例数为1296例,总感染率为21.14%。共检测出23种基因亚型,其中高危型18种、低危型5种。高危型HPV52及16感染率最高,分别为11.46%和10.85%,高危型出现次数的排序:52>16>58>53>51;低危型HPV6感染率最高,其次为HPV43。20~29岁及30~39岁组感染率远高于其他年龄组,差异有统计学意义。结论天津市妇女HPV52、16型感染最常见,年轻妇女HPV感染率最高,HPV分型检测在宫颈癌的早期预防、早期诊断具有重要意义。%Objective To provide theoretical basis for the development of vaccine and the treatment of cervical cancer, the status of human papillomavirus (HPV) in cervical cells of Tianjin women was investigated. Methods HPV genotype in 6 132 screening women was detected by human papillomavirus polymerase chain reaction (PCR) types diagnostic kit. The infection rate, genotype and age distribution in the female were analyzed. ResultsThe HPV infection women was 1 296 in screening 6 132 cases and the total infection rate was 21.14%. 23 genotypes were found. 18 kinds of the high risk and 5 kinds of low risk HPV genotypes were detected. The top risk genotypes were HPV52 and HPV16, and their infection rates were 11.46% and 10.85%, respectively. The infection rate of high risk HPV sorted as HPV52>16>58>53>51. The infection rate of HPV6 was highest in the low risk genotypes and was in the second rate for HPV43. The infection rate of 20~29 and 30~39 groups was much higher than that of the other

  2. Analysis and Detection of Subtype Distribution of Human Papillomavirus Genotype in 347 Patients with Condyloma Acuminatum%347例尖锐湿疣患者人乳头瘤病毒基因型检测及分析

    Institute of Scientific and Technical Information of China (English)

    凌勇活; 孙菲; 张霞; 阮建波

    2016-01-01

    Objective:To analyze the subtype distribution of Human Papillomavirus ( HPV ) genotypes in patients with Condyloma Acuminatum ( CA ) , and to present relevant findings for HPV prevention efforts .Methods:Lesions were excised from 347 patients with CA , and the flow-through hybridization technique was used to detect and classify 21 HPV genotypes according to the appropriate subtype distribution .Results:The overall prevalence of HPV in CA patients was 95.10%.All 21 genotypes of HPV were found .In the 347 samples , the main HPV genotypes were 6 ( 36.31%) , 11 ( 29.39%) , 16 ( 13.83%) , 52 ( 11.24%) , 58 ( 8.07%) , and 39 (8.07%).105 (31.82%) patients were found to have a single low-risk HPV infection, 92 (27.88%) patients with a single high-risk HPV infection and 133(40.30%) patients with multi-ple HPV infections .There were significant differences in single high-risk HPV infection and multi-ple HPV infection between female and male patients ( P0.05 ) .Conclusion:HPV 6 and HPV 11 were the most com-monly found genotypes , followed by HPV16, HPV52, HPV58, HPV39.Identification of the most prevalent genotypes is significant for efforts to develop preventative vaccines in Dongguan .%目的:分析尖锐湿疣(CA)患者人乳头瘤病毒(HPV)基因型的感染情况,为制定HPV感染的预防策略提供理论依据。方法:采用核酸分子快速导流杂交基因芯片技术,对347例尖锐湿疣患者皮损组织进行HPV感染21种基因亚型检测。结果:347份 CA 组织标本中 HPV 阳性330例,检出率95.10%,21种HPV亚型均能检测出。排名位于前6位的亚型检出率由高到低依次是HPV6(36.31%,126/347)、HPV11(29.39%,102/347)、HPV16(13.83%,48/347)、HPV52(11.24%,39/347)、HPV58(8.07%,28/347)、HPV39(8.07%,28/347)。单一低危型感染占31.82%(105/330),单一高危型感染占27.88%(92/330),多重感染占40.30%(133/330)。不同

  3. Soils distribution model based on relation between geology, geomorphology and pedology, at the High Plateau of Distrito Federal, Brazil

    Directory of Open Access Journals (Sweden)

    Inara Oliveira Barbosa

    2010-06-01

    Full Text Available Studies of the relation between geology and geomorphic surfaces are important for understanding the soil distribution in the landscape. In the Distrito Federal the scale survey does not evidence some pedologic features. This study aimed to generate a pedological distribution model in Distrito Federal High Plateau with more detailed scale, from the relations between geology, geomorphology and soils. The study area is located in the midwest portion of the Distrito Federal. To generate the soil map it was used geoprocessing tools (GIS supported by field checking. The soil map was obtained from crossing the geoforms (hypsometric and slope with the geology map. The established pattern of soil distribution in the Distrito Federal High Plateau was shown to be representative. The study allowed establishing the relations between the soil classes with geomorphology and defining altimetry classes, slope and geology on the soil occurrence.

  4. Avaliação de genótipos de triticale e trigo em ambientes favoráveis e desfavoráveis no Estado de São Paulo Evaluation of triticale and wheat genotypes under favorable and unfavorable environments in the State of São Paulo, Brazil

    Directory of Open Access Journals (Sweden)

    JOÃO CARLOS FELICIO

    2001-01-01

    Full Text Available Avaliou-se a adaptação de 18 genótipos de triticale e dois de trigo em experimentos instalados em dois ambientes, na Estação Experimental de Agronomia do Instituto Agronômico, localizada em Mococa (SP, no período de 1996-99. No primeiro ambiente, aplicou-se a irrigação por aspersão em condição de sequeiro (favorável e, no segundo, várzea úmida com irrigação por inundação (desfavorável. A diversidade entre os ambientes propiciou diferença entre os padrões de resposta dos genótipos. No ambiente irrigado por aspersão, houve maior produtividade para ambas as espécies, sendo o genótipo de triticale TATU 4/ARDI 1 o que apresentou o melhor rendimento de grãos. Na várzea úmida, destacou-se o triticale LT 978.82/ASAD//TARASCA. O IAC 60 (trigo apresentou grande variação no rendimento de grãos de um ano para outro, nas condições de várzea. O ciclo das plantas, da emergência ao espigamento, foi mais longo entre os genótipos de triticale nas condições de várzea úmida, quando comparado ao cultivo irrigado por aspersão. Esses resultados indicam que os genótipos de trigo apresentam adaptação específica para o ambiente de sequeiro com irrigação por aspersão, e o grupo de genótipos de triticale, adaptação mais ampla.The environment adaptation of eighteen triticale and two wheat genotypes were evaluated in experiments carried out at two different sites of the Mococa Agronomy Experimental Station, São Paulo State, Brazil, in the period 1996-99. The favorable environment was provided with sprinkler irrigation on an upland; the unfavorable condition consisted of a moist flat area with basin irrigation system. The environment diversity induced different genotypes response patterns. Under the sprinkler irrigation system the yields were higher for both species, but the triticale genotype TATU 4/ARDI 1 presented the highest grain yield. Under moist and flat area condition the triticale LT 978.82/ASAD//TARASCA showed

  5. USE OF THE DE LIOCOURT QUOTIENT IN THE EVALUATION OF THE DIAMETRIC DISTRIBUTION IN FRAGMENTS OF OMBROPHYLOUS FOREST, PERNAMBUCO STATE-BRAZIL

    Directory of Open Access Journals (Sweden)

    Francisco Tarcísio Alves Junior

    2010-08-01

    Full Text Available Great part of the biodiversity of the ecosystem of tropical forests is being lost even before we have full knowledge of its natural wealth, making it important perform studies that can provide knowledge and the maintenance of its structure, besides making possible the exploration of its products, goods and/or services in a planned and rational form, guaranteeing the continuous flow of these resources. The general objective of this study was to use the De Liocourt quotient in the evaluation of the diametric distribution in fragments of Ombrophylous Forest located in the city of Catende, Pernambuco state - Brazil, having as specific objectives: to describe the diametric structure of the fragments and the species of greater importance using the Value of Importance Index (VI; and to evaluate the degradation and the state of succession of the studied areas. The diametric distribution revealed uneven-aged forests as the diametric curve of distribution resembled a reverse J-shape. The values of basal area in the fragments were of 23.6 and 20.9 m2.ha-1, for Mata das Caldeiras and Mata das Galinhas. The fragments were, on average, in the secondary period of succession. Some species presented difficulties in the rate of recruitment, which could lead to the extinguishing of some species in the future. Species, such as Tapirira guianensis Aubl., Dialium guianense (Aubl. Sandwith and Brosimum discolor Schott, demonstrated a distinct diametric structure among the fragments. The species Plathymenia foliolosa Benth. presented accented discontinuities in the diametric structure in both areas.

  6. Spatial distribution pattern of Mezilaurus itauba (Meins. Taub. Ex mez. in a seasonal forest area of the southern Amazon, Brazil

    Directory of Open Access Journals (Sweden)

    Ebert A

    2016-06-01

    Full Text Available Spatial analysis of forest tree distribution is a powerful tool to respond to basic ecological questions, and represent a useful support to strategies of genetic conservation and sustainable management practices of forest resources. Spatial analysis techniques combined with the use of Geographical Information Systems have been commonly applied to the study of stochastic processes in order to determine the existence of clusters to be related to microenviromental conditions and/or genetic factors. The present study focused on the distribution patterns of individuals of Mezilaurus itauba in a seasonal forest of the southern Amazon, with the aim of providing information about the spatial arrangement of these species at the juvenile and adult stages. Ripley’s K function with radius of 10, 20 and 30 m was used to describe spatial distribution patterns. The hypothesis of complete spatial randomness (CSR of individuals was tested by constructing confidence envelopes for the Ripley’s K function through Monte Carlo simulations using a Poisson homogeneous process. The results obtained suggest a general random distribution of individuals, though a tendency to clustering at close distances was detected for individuals classified as adults (DBH > 50 cm. Contrastingly, a completely randomized spatial pattern was found for juveniles trees (DBH < 50 cm. Our results provide a useful baseline for the development of sustainable management plans and conservation of Mezilaurus itauba, as well as for other economically-exploited, native tree species in the southern Amazon forest.

  7. Caracterização de genótipos de trigo do bloco de cruzamento da Embrapa Trigo, RS, Brasil Characterization of wheat genotypes from the crossing block of the national wheat research center, RS, Brazil

    Directory of Open Access Journals (Sweden)

    Gilberto Peripolli Bevilaqua

    2003-10-01

    Full Text Available No Centro Nacional de Pesquisa de Trigo (CNPT - Embrapa Trigo, em 1999, genótipos de trigo integrantes do bloco de cruzamento foram avaliados quanto à altura de planta, à duração do ciclo até o espigamento, ao comprimento do pedúnculo e ao número de grãos por espiga. Relacionando essas características, foram estudados 122 genótipos em duas épocas de semeadura, que consistiram os blocos. Foram medidos dez pedúnculos do afilho principal, de cada genótipo, e contou-se o número total de grãos de cada espiga. Para a apresentação dos resultados, foram considerados os valores médios dessas avaliações. Para a análise estatística, considerou-se o experimento como blocos casualizados. Dos resultados obtidos, concluiu-se que os genótipos de trigo BR 18, BR 42 e Sonora 64 destacam-se por porte baixo e ciclo precoce, enquanto Florida 301 e "Trigo de chapéu" destacam-se por porte alto e ciclo longo até o espigamento. A altura de planta mostra elevada correlação com comprimento do pedúnculo e duração do ciclo até o espigamento, e esta, com o comprimento de pedúnculo. Os genótipos de trigo CNT 10 e PF 92130 são indicados com finalidades artesanais por suas características de pedúnculo longo e número de grãos por espiga acima da média. O estudo mostra grande variabilidade genotípica em trigo quanto às características avaliadas, fornecendo informações sobre os genótipos que poderão ser utilizados por melhoristas, técnicos e agricultores.Wheat genotypes from the crossing block were caracterized at the National Wheat Research Center (Embrapa Trigo in 1999. Plant traits evaluated were height, cycle up to heading, peduncle length, and number of grains per spike. One hundred and twenty-two genotypes were evaluated at two seeding dates that consisted blocks. For each tretament, ten peduncles from the main tiller were measured and the total number of grain in each spike was counted. The mean values obtained in such

  8. Modeling of spatial distribution for scorpions of medical importance in the São Paulo State, Brazil

    Directory of Open Access Journals (Sweden)

    José Brites-Neto

    2015-07-01

    Full Text Available Aim: In this work, we aimed to develop maps of modeling geographic distribution correlating to environmental suitability for the two species of scorpions of medical importance at São Paulo State and to develop spatial configuration parameters for epidemiological surveillance of these species of venomous animals. Materials and Methods: In this study, 54 georeferenced points for Tityus serrulatus and 86 points for Tityus bahiensis and eight environmental indicators, were used to generate species distribution models in Maxent (maximum entropy modeling of species geographic distributions version 3.3.3k using 70% of data for training (n=38 to T. serrulatus and n=60 to T. bahiensis and 30% to test the models (n=16 for T. serrulatus and n=26 for T. bahiensis. The logistic threshold used to cut models in converting the continuous probability model into a binary model was the “maximum test sensitivity plus specificity,” provided by Maxent, with results of 0.4143 to T. serrulatus and of 0.3401 to T. bahiensis. The models were evaluated by the area under the curve (AUC, using the omission error and the binomial probability. With the data generated by Maxent, distribution maps were produced using the “ESRI® ArcGIS 10.2.2 for Desktop” software. Results: The models had high predictive success (AUC=0.7698±0.0533, omission error=0.2467 and p<0.001 for T. serrulatus and AUC=0.8205±0.0390, omission error=0.1917 and p<0.001 for T. bahiensis and the resultant maps showed a high environmental suitability in the north, central, and southeast of the state, confirming the increasing spread of these species. The environmental variables that mostly contributed to the scorpions species distribution model were rain precipitation (28.9% and tree cover (28.2% for the T. serrulatus and temperature (45.8% and thermal amplitude (12.6% for the T. bahiensis. Conclusion: The distribution model of these species of medical importance scorpions in São Paulo State

  9. Distribution and conservation of annual fishes (Cyprinodontiformes: Rivulidae in the municipality of Chuí, southern Brazil

    Directory of Open Access Journals (Sweden)

    Matheus Vieira Volcan

    2010-12-01

    Full Text Available This study is based on captures of annual fishes conducted from August 2004 to June 2009, aiming at the dissemination of data on their occurrence, distribution and conservation in the municipality of Chuí. Four species were recorded: Austrolebias charrua Costa e Cheffe, Austrolebias luteofl ammulatus (Vaz-Ferreira, Sierra-de-Soriano e Scaglia-de-Paulete, Austrolebias prognathus (Amato and Cynopoecilus melanotaenia (Regan, captured at 14 different sampling points distributed in the floodplains of Chuí and São Miguel streams, near the road of Barra do Chuí. All ponds where annual fishes were recorded in Chuí were altered by some form of human activity, arising mainly from rice culture and trampling by cattle. The main area of annual fish occurrence in the municipality that is most relevant to conservation is located in the floodplain of the Chuí stream.

  10. Diversity and distribution of sandflies (Diptera: Psychodidae: Phlebotominae in a military area in the state of Amazonas, Brazil

    Directory of Open Access Journals (Sweden)

    Luís Henrique Monteiro Gomes

    2013-08-01

    Full Text Available This study reports the distribution, ecotopes and fauna diversity of sandflies captured in five training bases on a military reserve in Manaus, state of Amazonas (AM. A total of 10,762 specimens were collected, which were distributed among 58 species, with the highest number recorded at Base Instruction 1 (BI1. A higher rate of species richness was found at the Base Instruction Boina Rajada and low levels of diversity associated with a high abundance index with the clear dominance of Lutzomyia umbratilis, Lutzomyia ruii and Lutzomyia anduzei were found at BI1. The abundance of Lu. umbratilis raises the possibility of outbreaks of American cutaneous leishmaniasis by the main vector of the disease in AM.

  11. Spatial distribution pattern of Mezilaurus itauba (Meins.) Taub. Ex mez. in a seasonal forest area of the southern Amazon, Brazil

    OpenAIRE

    Ebert A; Brito Da Costa R; Brondani GE

    2016-01-01

    Spatial analysis of forest tree distribution is a powerful tool to respond to basic ecological questions, and represent a useful support to strategies of genetic conservation and sustainable management practices of forest resources. Spatial analysis techniques combined with the use of Geographical Information Systems have been commonly applied to the study of stochastic processes in order to determine the existence of clusters to be related to microenviromental conditions and/or genetic facto...

  12. Distribution and growth in adults of Macrobrachium acanthurus Wiegmann, (Decapoda, Palaemonidae) in a tropical coastal lagoon, Brazil

    OpenAIRE

    Edélti Faria Albertoni; Cleber Palma-Silva; Francisco de Assis Esteves

    2002-01-01

    Macrobrachium acanthurus Wiegmann, 1836 is a Palaemonidae commonly found in Brazilian coastal environments. At Imboassica lagoon, located in the north of the State of Rio de Janeiro, it is found in two stages of its life cycle: as larvae and as reproductive adults. This work had as its goal the evaluation of adults distribution, estimating the weight/length relationship and the condition factor of these adults. After sampling in two regions of the lagoon, one interior and another closer to th...

  13. High sensitivity C-reactive protein distribution in the elderly: the Bambuí Cohort Study, Brazil

    International Nuclear Information System (INIS)

    The measurement of the serum concentration of the acute-phase reactant C-reactive protein (CRP) provides a useful marker in clinical practice. However, the distribution of CRP is not available for all age and population groups. This study assessed the distribution of high sensitivity CRP (hs-CRP) by gender and age in 1470 elderly individuals from a Brazilian community that participates in the Bambuí Cohort Study. Blood samples were collected after 12 h of fasting and serum samples were stored at -70°C. Measurements were made with a commercial hs-CRP immunonephelometric instrument. More than 50% of the results were above 3.0 mg/L for both genders. Mean hs-CRP was higher in women (3.62 ± 2.58 mg/L) than in men (3.03 ± 2.50 mg/L). This difference was observed for all ages, except for the over-80 age group. This is the first population-based study to describe hs-CRP values in Latin American elderly subjects. Our results indicate that significant gender differences exist in the distribution of hs-CRP, and suggest that gender-specific cut-off points for hs-CRP would be necessary for the prediction of cardiovascular risks

  14. High sensitivity C-reactive protein distribution in the elderly: the Bambuí Cohort Study, Brazil

    Energy Technology Data Exchange (ETDEWEB)

    Assunção, L.G.S. [Programa de Pós-Graduação em Patologia, Faculdade de Medicina, Universidade Federal de Minas Gerais, Belo Horizonte, MG (Brazil); Eloi-Santos, S.M. [Programa de Pós-Graduação em Patologia, Faculdade de Medicina, Universidade Federal de Minas Gerais, Belo Horizonte, MG (Brazil); Departamento de Propedêutica Complementar, Faculdade de Medicina, Universidade Federal de Minas Gerais, Belo Horizonte, MG (Brazil); Peixoto, S.V. [Departamento de Enfermagem Aplicada, Escola de Enfermagem, Universidade Federal de Minas Gerais, Belo Horizonte, MG (Brazil); Centro de Pesquisas René Rachou, Fundação Oswaldo Cruz, Belo Horizonte, MG (Brazil); Lima-Costa, M.F. [Departamento de Medicina Preventiva e Social, Universidade Federal de Minas Gerais, Belo Horizonte, MG (Brazil); Centro de Pesquisas René Rachou, Fundação Oswaldo Cruz, Belo Horizonte, MG (Brazil); Vidigal, P.G. [Programa de Pós-Graduação em Patologia, Faculdade de Medicina, Universidade Federal de Minas Gerais, Belo Horizonte, MG (Brazil); Departamento de Propedêutica Complementar, Faculdade de Medicina, Universidade Federal de Minas Gerais, Belo Horizonte, MG (Brazil)

    2012-09-28

    The measurement of the serum concentration of the acute-phase reactant C-reactive protein (CRP) provides a useful marker in clinical practice. However, the distribution of CRP is not available for all age and population groups. This study assessed the distribution of high sensitivity CRP (hs-CRP) by gender and age in 1470 elderly individuals from a Brazilian community that participates in the Bambuí Cohort Study. Blood samples were collected after 12 h of fasting and serum samples were stored at -70°C. Measurements were made with a commercial hs-CRP immunonephelometric instrument. More than 50% of the results were above 3.0 mg/L for both genders. Mean hs-CRP was higher in women (3.62 ± 2.58 mg/L) than in men (3.03 ± 2.50 mg/L). This difference was observed for all ages, except for the over-80 age group. This is the first population-based study to describe hs-CRP values in Latin American elderly subjects. Our results indicate that significant gender differences exist in the distribution of hs-CRP, and suggest that gender-specific cut-off points for hs-CRP would be necessary for the prediction of cardiovascular risks.

  15. High sensitivity C-reactive protein distribution in the elderly: the Bambuí Cohort Study, Brazil

    Directory of Open Access Journals (Sweden)

    L.G.S. Assunção

    2012-12-01

    Full Text Available The measurement of the serum concentration of the acute-phase reactant C-reactive protein (CRP provides a useful marker in clinical practice. However, the distribution of CRP is not available for all age and population groups. This study assessed the distribution of high sensitivity-CRP (hs-CRP by gender and age in 1470 elderly individuals from a Brazilian community that participates in the Bambuí Cohort Study. Blood samples were collected after 12 h of fasting and serum samples were stored at -70°C. Measurements were made with a commercial hs-CRP immunonephelometric instrument. More than 50% of the results were above 3.0 mg/L for both genders. Mean hs-CRP was higher in women (3.62 ± 2.58 mg/L than in men (3.03 ± 2.50 mg/L. This difference was observed for all ages, except for the over-80 age group. This is the first population-based study to describe hs-CRP values in Latin American elderly subjects. Our results indicate that significant gender differences exist in the distribution of hs-CRP, and suggest that gender-specific cut-off points for hs-CRP would be necessary for the prediction of cardiovascular risks.

  16. Genotypes Distribution among Hepatitis B Virus Infectied Patients with Different Immune Statuses in Guangxi North Region%广西桂北地区HBV感染者不同免疫状态HBV基因型分布研究

    Institute of Scientific and Technical Information of China (English)

    杨丽莎; 吴淋玲; 蒋冬香; 王绩业; 黄亚琴

    2012-01-01

    In order to find out the distribution of Genotype of those people infected with HBV(hepatitis B virus )from north Guangxi and the relationship between different immune status of HBV infected people and their genotypes, the HBV infected people are classified into three types according to immune tolerance, immune clearance ( response ) and immune incompetence (residues) . 150 cases from each type, a total of 450 cases are chosen to be tested with real time fluorescence quantitative PCR assay for detection of HBV infection in three kinds of different immune state of the HBV genotype. In the 450 cases, 323 cases belong to type B, 94 cases belong to type B, 23 cases belong to mixed type B+C and 10 cases belong to none B and none C type. Type B are the majority in all the three HBV immunestatus, made up to 70%, 78% , 67. 33% of each type. The different immune state genotype proportion difference don't have statistical significance; immune state and genotypic correlation isn't statistically significant; type B HBV-DNA load is higher than that of type C, groups of persons aged 30 years or older with type C are significantly higher than that of < 30 years of age, the difference was statistically significant; among the genotypes of alanine aminotransferase ( ALT ) , aspartate aminotransferase ( AST ) , total bilirubin ( Tbil ) positive rate showed no significant difference between male and female; there was no significant difference in genotype distribution. The results show that, in North Guangxi HBV genotypes B, C accounts for the proportion, a small amount of B+C hybrid, occasionally fails to type HBV infection; among immune tolerance, immune clearance ( response ) and immune incompetence ( residues ) type B are in majority in these three kinds of immune state, chronic HBV infection immunity with the HBV genotype correlations were not statistically significant.%为了了解广西桂北地区乙型肝炎病毒(Hepatitis B Virus,HBV)感染者基因型分布情况及探

  17. Genotype Distribution of Human Papillomavirus among Women with Cervical Cytological Abnormalities or Invasive Squamous Cell Carcinoma in a High-Incidence Area of Esophageal Carcinoma in China.

    Science.gov (United States)

    Wang, Yuanyuan; Wang, Shaohong; Shen, Jinhui; Peng, Yanyan; Chen, Lechuan; Mai, Ruiqin; Zhang, Guohong

    2016-01-01

    Data of HPV genotype including 16 high-risk HPV (HR-HPV) and 4 low-risk HPV from 38,397 women with normal cytology, 1341 women with cervical cytology abnormalities, and 223 women with ISCC were retrospectively evaluated by a hospital-based study. The prevalence of high-risk HPV (HR-HPV) was 6.51%, 41.83%, and 96.86% in women with normal cytology, cervical cytology abnormalities, and ISCC, respectively. The three most common HPV types were HPV-52 (1.76%), HPV-16 (1.28%), and HPV-58 (0.97%) in women with normal cytology, whereas the most prevalent HPV type was HPV-16 (16.85%), followed by HPV-52 (9.55%) and HPV-58 (7.83%) in women with cervical cytology abnormalities. Specifically, HPV-16 had the highest frequency in ASC-H (24.16%, 36/149) and HSIL (35.71%, 110/308), while HPV-52 was the most common type in ASC-US (8.28%, 53/640) and LSIL (16.80%, 41/244). HPV-16 (75.78%), HPV18 (10.31%), and HPV58 (9.87%) were the most common types in women with ISCC. These data might contribute to increasing the knowledge of HPV epidemiology and providing the guide for vaccine selection for women in Shantou. PMID:27610364

  18. Analysis of phenotype, genotype and serotype distribution in erythromycin-resistant group B streptococci isolated from vaginal flora in Southern Ireland.

    LENUS (Irish Health Repository)

    Kiely, R A

    2010-02-01

    The screening of 2000 women of childbearing age in Cork between 2004 and 2006 produced 37 erythromycin-resistant group B streptococcus (GBS) isolates. PCR analysis was performed to determine the basis for erythromycin resistance. The ermTR gene was most frequently expressed (n = 19), followed by the ermB gene (n = 8). Four isolates harboured the mefA gene. Six isolates yielded no PCR products. Some phenotype-genotype correlation was observed. All isolates expressing the mefA gene displayed the M phenotype whilst all those expressing ermB displayed the constitutive macrolide resistance (cMLS(B)) phenotype. Of 19 isolates that expressed the ermTR gene, 16 displayed the inducible macrolide resistance (iMLS(B)) phenotype. Serotype analysis revealed that serotypes III and V predominated in these isolates. The identification of two erythromycin-resistant serotype VIII isolates among this collection represents the first reported finding of erythromycin resistance in this serotype. A single isolate was non-typable using two latex agglutination serotyping kits.

  19. Distribution of 226Ra, 232Th and 40K in soils and sugar cane crops at Corumbatai river basin, Sao Paulo State, Brazil

    International Nuclear Information System (INIS)

    The common use of phosphate fertilizers NPK and amendments in sugar cane crops in Brazilian agriculture may increase the 226Ra, 232Th and 40K activity concentrations in soils and their availability for plants and human food chain. Thus, the main aim of this study was to evaluate the distribution of 226Ra, 232Th and 40K in soils and sugar cane crops in the Corumbatai river basin, Sao Paulo State, Brazil. The gamma spectrometry was utilized to measure the 226Ra, 232Th and 40K activity concentration in all samples. The soil-to-sugar cane transfer factors (TF) were quantified using the ratio between the radionuclide activity concentration in sugar cane and its activity concentration in soil. The results show that, although radionuclides incorporated in phosphate fertilizers and amendments are annually added in the sugar cane crops, if utilized in accordance with the recommended rates, their use does not lead to hazards levels in soils. The soil-to-sugar cane transfer of radionuclides occurred in the following order 40K>226Ra>232Th. Therefore, under these conditions, radionuclides intake through consumption of sugar is not hazardous to human health.

  20. Distribution of soil gas radon concentration in the metropolitan region of Belo Horizonte, Minas Gerais, Brazil and correlations with lithologies and pedologies

    Energy Technology Data Exchange (ETDEWEB)

    Lara, Evelise G.; Santos, Talita de O.; Oliveira, Arno Heeren de, E-mail: heeren@nuclear.ufmg.br [Universidade Federal de Minas Gerais (UFMG), Belo Horizonte, MG (Brazil). Escola de Engenharia. Dept. de Engenharia Nuclear; Rocha, Zildete; Miguel, Ronaldo Araujo; Dutra Neto, Aimore; Menezes, Maria Angela de B.C., E-mail: rochaz@cdtn.br, E-mail: menezes@cdtn.br, E-mail: dutraa@cdtn.br, E-mail: ram@cdtn.br [Centro de Desenvolvimento da Tecnologia Nuclear (CDTN/CNEN-MG), Belo Horizonte, MG (Brazil)

    2011-07-01

    The assessment of data from a previous study of radon in indoor air of about 500 built 'slab-on-grade' dwellings in the metropolitan area of Belo Horizonte, RMBH, Brazil, showed some statistical correlations with lithologies and pedologies. Forty-five measurements of radon concentration in soil gases were performed at selected points according to population density, gamma radiation background and measurements results of the mentioned indoor radon concentrations study. This paper presents the distribution of radon concentration in soil gas in the same area, where it ranges from 7 - 93 kBq.m{sup -3}, highlighting the areas of high radon concentration in soil gas, which suggests occurrence of radon prone areas. In this regard, this work looking for correlation of radon concentrations in soil gas with lithologies and pedologies. The measurements were performed by using AlphaGUARD PQ2000 PRO SAPHYMO GmbH. The results of a radon survey in this areas has indicated that in the region radon prone areas may occur. Radon concentration measurements in soil gas are also being carried out to obtain higher statistical significance data for correlations and for comparison between equipment and probes from different systems suppliers like RTM 1688 of SARAD GmbH and RAD7 Electronic Radon Detector of DURRIDGE Company Inc. Thus, this research will represent the initial contribution to establish classification criteria, for radon in soil gas in a tropical climate similar to the Swedish criteria established by Akerblom. (author)

  1. Evapotranspiration and energy balance components spatial distribution in the north region of Minas Gerais, Brazil, using the SEBAL model and Landsat 5 TM images

    Science.gov (United States)

    Gomide, Reinaldo L.; de Paula Boratto, Isa Maria

    2014-10-01

    The determination of crop evapotranspiration (ETc) values is very useful information for planning irrigation, water supply estimation, regulation of water rights and river basins hydrologic studies. Values of ETc in the North region of Minas Gerais state, Brazil, were estimated in this research from the multispectral images of the Landsat 5 TM by means of the model Surface Energy Balance Algorithm for Land- SEBAL, based on the simplified energy balance equation of a surface covered by vegetation, using a few daily surface climatological parameters (wind speed, rainfall, air temperature and relative humidity, solar radiation). The aim of this study was to estimate the regional spatial distribution of the energy balance components and evapotranspiration in the study area, covering the irrigated perimeter of Gorutuba, involving the cities of Nova Porteirinha, Janaúba, Porteirinha, Verdelândia and Pai Pedro. Thematic maps of regional evapotranspiration and energy balance components were generated from spectral analyzes of the images obtained, associated with the used weather data. The ability of SEBAL to provide the spatial variability of energy balance components, including evapotranspiration, demonstrated its sensitivity to different occupation of the soil surface vegetation, and to high data temporal and spatial resolutions data, indicating that the SEBAL model can be used in scales and operational routine for north region of Minas Gerais.

  2. A new dwarf species, new distribution records, and supplementary descriptive notes of the centipede genus Ityphilus Cook, 1899 (Chilopoda: Geophilomorpha: Ballophilidae from central Amazonia, Brazil

    Directory of Open Access Journals (Sweden)

    Luis Alberto Pereira

    2012-01-01

    Full Text Available A new dwarf species of the centipede genus Ityphilus Cook, 1899, named I. donatellae sp. nov. (Chilopoda: Geophilomorpha: Ballophilidae is hereby proposed on the basis of specimens from the vicinity of Manaus, Central Amazonia (Brazil, previously identified as Ityphilus calinus Chamberlin, 1957 (hereby designated holotype female, paratype male and paratype female. Supplementary morphological data and new illustrations are provided after this type material. The new species, characterized by having the internal edge of the forcipular tarsungulum serrate, is herein included in a key that will enable the identification of the 10 other Neotropical members of the genus Ityphilus sharing the same trait. New distribution records and supplementary descriptive notes for Ityphilus crabilli Pereira, Minelli & Barbieri, 1994, and Ityphilus demoraisi Pereira, Minelli & Barbieri, 1995 (including the first description of the male of the latter, are also given. Undiluted 2-Phenoxyethanol (CAS No. 122-99-6 has been used as an effective clearing agent/mounting medium for the preparation of temporary mounts of all body parts of the examined specimens.

  3. Distribution and origin of polycyclic aromatic hydrocarbons in surface sediments from an urban river basin at the Metropolitan Region of Curitiba, Brazil

    Institute of Scientific and Technical Information of China (English)

    Natalicio Ferreira Leite; Patricio Peralta-Zamora; Marco Tadeu Grassi

    2011-01-01

    The concentrations of 16 polycyclic aromatic hydrocarbons (PAHs) were determined in surface sediment samples from nine sites located at the Iguacu River Basin in the Metropolitan Region of Curitiba, Brazil to evaluate their distribution and sources.The total concentration of the PAHs was greater for sediments from highly urbanized areas, while the sediments from the IraíEnvironmental Protection Area (IraíEPA) showed significantly low concentrations.The sediments from the Iguacu and Barigui rivers were classified as highly contaminated, while those from the Cercado and Curralinho rivers were classified as moderately contaminated.The predominance of PAHs containing two to four aromatic rings in most of the samples suggested the direct input of raw sewage into the water resources evaluated.Benzo[g,h,i]perylene, dibenzo[a,h]anthracene and indeno[1,2,3-cd]pyrene were predominant in sediments from the areas under the greatest urban and industrial development.The correlation between thermodynamic stability and the kinetics of evolution presented by the isomeric pairs indicated that combustion is the predominant source of PAHs in the sediments because the combustion of fossil fuels affected most of the points evaluated, followed by combustion of biomass and eventually combustion of oil product inputs.In general, the results showed that areas under strong urban influence, as well as the IraíEPA, receive contributions of PAils from similar sources.

  4. Distribution and Schistosoma mansoni infection of Biomphalaria glabrata in different habitats in a rural area in the Jequitinhonha Valley, Minas Gerais, Brazil: environmental and epidemiological aspects

    Directory of Open Access Journals (Sweden)

    Helmut Kloos

    2004-11-01

    Full Text Available This paper examines the distribution and infection of Biomphalaria glabrata with Schistosoma mansoni in all aquatic snail habitats in a rural area in the state of Minas Gerais, Brazil, in relation to physico/biotic and behavioral factors. Snail and environmental surveys were carried out semi-annually between July 2001 and November 2002 at 106 sites. Collected snails were examined in the laboratory for infection. B. glabrata densities were highest in overflow ponds, irrigation ponds, springs, canals and wells, and lowest in fishponds and water tanks. Snail densities were higher during the hot, rainy season except for streams and canals and were statistically associated with the presence of fish, pollution, and vegetation density. Tilapia fish and an unidentified Diptera larva were found to be predators of B. glabrata but ducks were not. Twenty-four of the 25 infected snails were collected in 2001(1.4% infection rate and only one in 2002, after mass chemotherapy. The occurrence of B. glabrata in all 11 snail habitats both at and away from water contact sites studied indicates widespread risk of human infection in the study area. In spite of the strong association between B. glabrata and tilapia in fishponds we do not recommend its use in schistosomiasis control for ecological reasons and its relative inefficiency in streams and dams.

  5. Influence of effluents from a Wastewater Treatment Plant on nutrient distribution in a coastal creek from southern Brazil

    Directory of Open Access Journals (Sweden)

    Isaac Rodrigues Santos

    2008-02-01

    Full Text Available The hypothesis that effluents treated through activated sludge process cause changes in nutrient biogeochemistry of receiving water bodies was investigated in Vieira creek, southern Brazil. Dissolved oxygen, suspended matter, and pH did not vary among the sampling stations. Nutrient, biochemical oxygen demand, and conductivity values were significantly higher downstream from the Wastewater Treatment Plant (WWTP effluents. Further downstream, nitrate concentrations were higher due to ammonium nitrification, organic matter remineralization and/or the occurrence of unidentified sources. Per capita nutrient emission factors were estimated to be 0.16 kg P.yr-1 and 4.14 kg N.yr-1. Under pristine conditions, low N:P ratios were observed, which were significantly increased downstream due to the high ammonium input. The mixing zone of the nitrogen-rich waters from Vieira creek with the phosphorus-enriched waters from Patos lagoon estuary was considered under high risk of eutrophication. The results could be useful for planning and management of WWTP-effluent receiving waters in temperate regions from developing countries.A hipótese de que efluentes urbanos tratados através de um sistema de lodo ativado causam alterações na qualidade de água de ambientes aquáticos foi investigada no Arroio Vieira, Rio Grande, RS. Amostras de água foram coletadas a montante e a jusante dos emissários de uma estação de tratamento de esgoto (ETE. Oxigênio, material em suspensão e pH não variaram espacialmente. Já os valores para os nutrientes e para a demanda bioquímica do oxigênio foram significativamente maiores a jusante dos efluentes. Mais a jusante, as concentrações de nitrato aumentam devido à nitrificação do nitrogênio amoniacal, remineralização da matéria orgânica e/ou ocorrência de outros aportes não-identificados. A emissão de nutrientes per capita após o tratamento dos efluentes domésticos (0.16 kg P ano-1 e 4.14 kg N ano-1

  6. Legislation perspectives about social assistance programs for electricity distribution networks extensions. Considerations from ELETROPAULO case study, Sao Paulo, Brazil

    International Nuclear Information System (INIS)

    The central issue of debate was the need to align the energy sector's options and organization with changing global patterns of economic and social development, characterized by the increasing role played by the private sector, greater integration in the world economy, and new economic and social priorities such as efficiency, decentralization, deregulation, and a closer attention to environmental issues. The aim of the work was to present legislation perspectives about social assistance programs for electricity distribution networks extensions considering a Brazilian electric utility case study

  7. Genotypic variation among lineages of Trypanosoma cruzi and its geographic aspects.

    Science.gov (United States)

    Higo, Hiroo; Miura, Sachio; Horio, Masahiro; Mimori, Tatsuyuki; Hamano, Shinjiro; Agatsuma, Takeshi; Yanagi, Tetsuo; Cruz-Reyes, Arejandro; Uyema, Norma; Rojas de Arias, A; Matta, Vivian; Akahane, Hiroshige; Hirayama, Kenji; Takeuchi, Tsutomu; Tada, Isao; Himeno, Kunisuke

    2004-12-01

    Isozyme analysis with 18 enzyme loci was conducted on 146 isolates of Trypanosoma cruzi from Mexico, Guatemala, Colombia, Ecuador, Peru, Brazil, Bolivia, Paraguay and Chile. Forty-four different MLGs (groups of isolates with identical multilocus genotypes) were identified and a phylogeny was constructed. The phylogenetic tree consisted of two main groups (T. cruzi I, T. cruzi II), and the latter was further divided into two subgroups (T. cruzi IIa, T. cruzi IIb-e). Evidence of hybridization between different MLGs of T. cruzi II was found, which means that genetic exchanges seem to have occurred in South American T. cruzi. On the other hand, the persistence of characteristic T. cruzi I and T. cruzi II isozyme patterns in single small villages in Bolivia and Guatemala suggested that genetic exchange is very rare between major lineages. A significant difference in genetic diversity was shown between T. cruzi I and T. cruzi II from several indices of population genetics. Two possibilities could explain this genetic variation in the population: differences in evolutionary history and/or different tendencies to exchange genetic material. Broad-scale geographic distributions of T. cruzi I and T. cruzi IIb-e were different; T. cruzi I occurred in Central America and south to Bolivia and Brazil, while T. cruzi IIb-e occurred in the central and southern areas of South America, overlapping with T. cruzi I in Brazil and Bolivia.

  8. Detection of Mayaro virus infections during a dengue outbreak in Mato Grosso, Brazil.

    Science.gov (United States)

    Vieira, Carla Julia da Silva Pessoa; Silva, David José Ferreira da; Barreto, Eriana Serpa; Siqueira, Carlos Eduardo Hassegawa; Colombo, Tatiana Elias; Ozanic, Katia; Schmidt, Diane Johnson; Drumond, Betânia Paiva; Mondini, Adriano; Nogueira, Maurício Lacerda; Bronzoni, Roberta Vieira de Morais

    2015-07-01

    Arboviruses are common agents of human febrile illness worldwide. In dengue-endemic areas illness due to other arboviruses have been misdiagnosed as dengue based only on clinical-epidemiological data. In this study we investigated the presence of Brazilian arboviruses in sera of 200 patients presenting acute febrile illness, during a dengue outbreak in Sinop, MT, Brazil. The results showed that 38 samples were positive to Dengue virus (DENV) type 1, two samples to DENV type 4, and six to Mayaro virus. These results indicate that arboviruses others than DENV are circulating in Sinop and the surrounding region, which are going undiagnosed. In addition, molecular and evolutionary analyses indicate that two MAYV genotypes are co-circulating in Mato Grosso, Brazil. Thus, a strong surveillance program must be implemented to evaluate and monitor the distribution and the true importance of non-dengue arboviruses in the etiology of acute febrile illnesses.

  9. [Identification and genetic variability of annatto genotypes (Bixa orellana L.) by means of hydrosoluble proteins and isoenzymes].

    Science.gov (United States)

    Medina, A M; Michelangeli, C; Ramis, C; Díaz, A

    2001-01-01

    In order to identify and to determine the genetic variability of 36 annatto genotypes (Bixa orellana L.) collected in five Venezuelan regions (Oriente, Centro, Llanos, Andes and Amazonas) and in Brazil, hydrosoluble protein patterns as well as specific isozyme patterns (alpha-esterase, beta-esterase and peroxidase) were studied using extracts of germinated annatto seeds with radicles of 10 to 15 mm long. Each electrophoretic system allowed genotype discrimination by means of unique banding patterns: both the hydrosoluble protein and the electrophoretic system of beta-esterase with nine banding patterns each; whilst alpha-esterase and peroxidase discriminated eight and three genotypes, respectively. On the other hand, a combination of all the systems permitted a greater discrimination since 34 out of 36 genotypes could be distinguished. Eight mayor groups were formed that showed high levels of genetic diversity (40 to 60%) with no association between geographic and genetic distances, probably because of human influence in the aleatory distribution of this crop. Results obtained indicated that using electrophoretic banding patterns, a classification system could be established for identification and genetic variability purposes in this species. PMID:11510424

  10. Distribution and growth in adults of Macrobrachium acanthurus Wiegmann, (Decapoda, Palaemonidae in a tropical coastal lagoon, Brazil

    Directory of Open Access Journals (Sweden)

    Edélti Faria Albertoni

    2002-12-01

    Full Text Available Macrobrachium acanthurus Wiegmann, 1836 is a Palaemonidae commonly found in Brazilian coastal environments. At Imboassica lagoon, located in the north of the State of Rio de Janeiro, it is found in two stages of its life cycle: as larvae and as reproductive adults. This work had as its goal the evaluation of adults distribution, estimating the weight/length relationship and the condition factor of these adults. After sampling in two regions of the lagoon, one interior and another closer to the ocean, we verified that females are found in a greater proportion close to the ocean, and males are found in greater number in the innermost region, amidst the aquatic macrophytes, suggesting that the fecundation takes place in this last area and the females then travel to the area closer to the ocean to spawn. Total and carapace weight/length relationship exhibited differences between males and females, presenting positive allometric growth. The relative growth of the cephalotorax related to total length showed that it correspond to 53% of the total length in females, and 50% in males. The average condition factor was significantly lower than the expected pattern, demonstrating that the species does not find ideal conditions in the lagoon. This may be attributed to the migration of the adults, to the spawning and/or to the impact caused by the frequent sandbar openings that took place at Imboassica lagoon during the studied period.

  11. Mansonella ozzardi in Amazonas, Brazil: prevalence and distribution in the municipality of Coari, in the middle Solimões River

    Directory of Open Access Journals (Sweden)

    Marilaine Martins

    2010-05-01

    Full Text Available This study investigated some epidemiological aspects of the Mansonella ozzardi in municipality of Coari, Amazonas. Clinical symptoms were correlated with the filarial infection and the parasitic infection rates (PIR were estimated in simuliid vectors. The general M. ozzardi human prevalence rate was 13.3% (231/1733, of which 10.2% (109/1069 were from the urban area and 18.4% (122/664 from the rural area. The prevalence rates were higher in men (14.5% urban and 19.7% rural than in women (6.7% urban and 17.2% rural and occurred in most age groups. The indices of microfilaremics were higher in people > 51 years old (26.9% urban and 61.5% rural. High prevalence rates were observed in retired people (27.1% urban area, housewives and farmer (41.6% and 25%, respectively, in rural area. The main clinical symptoms were joint pains and sensation of leg coldness. Only Cerqueirellum argentiscutum (Simuliidae transmits M. ozzardi in this municipality (PIR = 5.6% urban and 7.1% rural. M. ozzardi is a widely distributed parasitic disease in Coari. Thus, temporary residency in the region of people from other localities involved with the local gas exploitation might be a contributing factor in spreading the disease.

  12. Mansonella ozzardi in Amazonas, Brazil: prevalence and distribution in the municipality of Coari, in the middle Solimões River.

    Science.gov (United States)

    Martins, Marilaine; Pessoa, Felipe Arley Costa; de Medeiros, Maurício Borborema; de Andrade, Edmar Vaz; Medeiros, Jansen Fernandes

    2010-05-01

    This study investigated some epidemiological aspects of the Mansonella ozzardi in municipality of Coari, Amazonas. Clinical symptoms were correlated with the filarial infection and the parasitic infection rates (PIR) were estimated in simuliid vectors. The general M. ozzardi human prevalence rate was 13.3% (231/1733), of which 10.2% (109/1069) were from the urban area and 18.4% (122/664) from the rural area. The prevalence rates were higher in men (14.5% urban and 19.7% rural) than in women (6.7% urban and 17.2% rural) and occurred in most age groups. The indices of microfilaremics were higher in people > or = 51 years old (26.9% urban and 61.5% rural). High prevalence rates were observed in retired people (27.1% urban area), housewives and farmer (41.6% and 25%, respectively, in rural area). The main clinical symptoms were joint pains and sensation of leg coldness. Only Cerqueirellum argentiscutum (Simuliidae) transmits M. ozzardi in this municipality (PIR = 5.6% urban and 7.1% rural). M. ozzardi is a widely distributed parasitic disease in Coari. Thus, temporary residency in the region of people from other localities involved with the local gas exploitation might be a contributing factor in spreading the disease. PMID:20512236

  13. Vertical distribution of zooplankton in the water column of Lago Amapá, Rio Branco, Acre, Brazil

    Directory of Open Access Journals (Sweden)

    Erlei Cassiano Keppeler

    2004-06-01

    Full Text Available The aim of investigation was to study the model of vertical distribution in Lago Amapá, taking into consideration the seasonality of its zooplanktonic composition. Lago Amapá (10º2'36"S and 67º50'24"W is located in the floodplain of the Rio Acre. Samplings were conducted at three different depths of the water column, to study the vertical distribution of zooplankton populations and determine some physico-chemical and biological parameters of Lago Amapá. Weekly samples were taken with a Van Dorn sampler. The species showed greater concentrations at the by means of water column. Thirty-eight zooplankton species were found in the samples represented by Rotifera (30, Cladocera (5 and Cyclopoida (3. The temperature of the water column showed a tendency toward relatively high values (about 30ºC with little variation, consequently resulting in low viscosity. Based of Jaccard's index, it was seen that during the low-water phase, S1 and S3 of the three sampling stations studied, had greater similarity (Cj = 0.7058 in the middle of the water column. Lago Amapá showed characteristics in line with the intermediate disturbance hypothesis model, favoring colonization by opportunistic species such as rotifers.O objetivo desta investigação foi observar a distribuição vertical da comunidade do zooplâncton no Lago Amapá (10º2'36"S e 67º50'24"W, localizado na planície de inundação do Rio Acre. Amostragens foram conduzidas em três diferentes profundidades da coluna da água, considerando aspectos sazonais do zooplâncton, parâmetros físicos, químicos e biológicos. Coletas foram realizadas semanalmente com Garrafa de Van Dorn. As espécies apresentaram maiores concentrações no meio da coluna da água. Foram encontradas 38 espécies, assim distribuídas: Rotifera (30, Cladocera (5 e Cyclopoida (3. A temperatura da coluna da água em geral apresentou-se alta, em torno de 30ºC, com pequena variação, resultando em baixa viscosidade. O

  14. Spatial distribution of the risk of dengue and the entomological indicators in Sumare, state of Sao Paulo, Brazil.

    Directory of Open Access Journals (Sweden)

    Gerson Laurindo Barbosa

    2014-05-01

    Full Text Available Dengue fever is a major public health problem worldwide, caused by any of four virus (DENV-1, DENV-2, DENV-3 and DENV-4; Flaviviridae: Flavivirus, transmitted by Aedes aegypti mosquito. Reducing the levels of infestation by A. aegypti is one of the few current strategies to control dengue fever. Entomological indicators are used by dengue national control program to measure the infestation of A. aegypti, but little is known about predictive power of these indicators to measure dengue risk. In this spatial case-control study, we analyzed the spatial distribution of the risk of dengue and the influence of entomological indicators of A. aegypti in its egg, larva-pupa and adult stages occurring in a mid-size city in the state of São Paulo. The dengue cases were those confirmed by the city's epidemiological surveillance system and the controls were obtained through random selection of points within the perimeter of the inhabited area. The values of the entomological indicators were extrapolated for the entire study area through the geostatistical ordinary kriging technique. For each case and control, the respective indicator values were obtained, according with its geographical coordinates and analyzed by using a generalized additive model. Dengue incidence demonstrated a seasonal behavior, as well as the entomological indicators of all mosquito's evolutionary stages. The infestation did not present a significant variation in intensity and was not a limiting or determining factor of the occurrence of cases in the municipality. The risk maps of the disease from crude and adjusted generalized additive models did not present differences, suggesting that areas with the highest values of entomological indicators were not associated with the incidence of dengue. The inclusion of other variables in the generalized additive models may reveal the modulatory effect for the risk of the disease, which is not found in this study.

  15. Paracoccidioidomycosis in children in the state of Rio de Janeiro (Brazil: Geographic distribution and the study of a "reservarea"

    Directory of Open Access Journals (Sweden)

    A. J. RIOS GONÇALVES

    1998-01-01

    Full Text Available Thirty six cases of acute disseminated paracoccidioidomycosis in 3 to 12 year-old children, natives of the state of Rio de Janeiro, were seen in the period 1981-1996. All patients were residents in the rural region of 15 counties, scattered on the Southwestern part of this state. The rural region of two neighboring counties, where 16 cases (44.4% occurred, was visited. It exhibited the environmental conditions that are considered favorable to the survival of P. brasiliensis. The most important of these conditions, abundant watercourses and autochthonous forest, are distributed on well defined and limited areas, in which the dwellings are also localized. Probably, a careful epidemiological study of forthcoming cases of the disease in children may facilitate the search for the micro-niche of the fungus.No período 1981-1996 foram observados 36 casos de paracoccidioidomicose aguda disseminada em crianças, entre 3 e 12 anos de idade, nativas do estado do Rio de Janeiro. Todos os pacientes residiam em região rural, distribuída por 15 municípios localizados na parte sudoeste do estado. Dois municípios vizinhos, onde haviam ocorrido 16 (44,4% dos casos, foram visitados. Na região rural de ambos, existem as condições climáticas e ambientais favoráveis à sobrevivência do P. brasiliensis. Dentre essas condições, as mais importantes, abundantes cursos de água e vegetação nativa, distribuem-se em áreas limitadas e bem definidas. Nessas áreas localizam-se, também, as casas dos rurículas. Provavelmente, um cuidadoso estudo epidemiológico de novos casos, que venham a ocorrer na região, facilitará a busca do nicho do fungo

  16. Identificação de Genótipos de Feijão-caupi Tolerantes a Acidez em um Latossolo Vermelho-Amarelo do Estado de Roraima = Identification of cowpea genotypes sensitivity to acidicy conditions in an Oxisol of Roraima State (Brazil.

    Directory of Open Access Journals (Sweden)

    Sandra Cátia Pereira Uchôa

    2007-12-01

    Full Text Available O presente trabalho foi realizado com o objetivo de identificar genótipos de feijão-caupi (Vigna unguiculata (L Walp., tolerantes à acidez. O trabalho foi realizado em casa de vegetação localizada no Campus do Cauamé, da Universidade Federal de Roraima, utilizando um Latossolo Vermelho-Amarelo de Boa Vista-RR. Foi adotado o delineamento experimental de Blocos Inteiramente Casualizados em esquema fatorial 2 x 5 x 10 com três repetições. Os tratamentos resultaram de duas formas de localização do calcário (localizada e não-localizada, 5 profundidades (0-5; 5-10; 10-15; 15-20; 20-25 cm e 10 genótipos de feijão-caupi(IT85D-3428-4-R2,4-HM; Apiaú; Hikari Graúdo; Pretinho Precoce 1; IT85D-3428-4-3-HP; USA; UFRR Grão Verde; BRS-Mazagão; Canapum e Sempre Verde. A unidade experimental foi constituída por 2dm3 de solo, dispostos em tubo de PVC com 25cm de altura,10cm de diâmetro e duas plantas de feijão. Os tubos foram confeccionados por meio da junção de 5 anéis com 5 cm cada, sendo que no anel inferior foi colocada uma placa delgada de isopor para permitir a acomodação do solo. Aos 40 dias após a emergência, as plantas foram coletadas, sendo determinada à produção de matéria seca das raízes e parte aérea. Os genótipos IT85D-3428-4-3-HP e UFRRGRÃO VERDE foram classificados como sendo de baixa tolerância, os genótipos USA, BRS-Mazagão, IT85D-3428-4-R2,4-HM e Sempre Verde, medianamente tolerantes e os genótipos Apiaú, Hikari Graúdo, Pretinho Precoce 1 e Canapum, tolerantes à acidez.= This research was carried out objecting to identify the sensibility of cowpea (Vigna unguiculata (L Walp genotypes to acidity conditions. The experiment was installed in a greenhouse located at Campus of Cauamé (Universidade Federal de Roraima in an oxisol of Roraima State (Brazil. The experimental design adopted was a completely andomized blocks in a factorial scheme 2 x 5 x 10 with three replicates. The terms of this factorial

  17. Influência do ambiente no rendimento e na qualidade de grãos de genótipos de trigo com irrigação por aspersão no Estado de São Paulo Environmental influence on grain yield and grain quality of wheat genotypes with sprinkler irrigation in the State of São paulo, Brazil

    Directory of Open Access Journals (Sweden)

    JOÃO CARLOS FELICIO

    2001-01-01

    Full Text Available Avaliaram-se a influência do ambiente em 20 genótipos de trigo, no rendimento e qualidade de grãos, a adaptabilidade e a reação a doenças, em regiões onde a irrigação se faz necessária para permitir o bom desempenho agronômico de um genótipo. Instalaram-se os experimentos em Tatuí, Votuporanga, Ribeirão Preto e Mococa, Estado de São Paulo, no período de 1995-98. Avaliaram-se, também, a tolerância dos genótipos ao alumínio tóxico em solução nutritiva e a qualidade industrial para panificação. Com bom rendimento de grãos destacaram-se: em Tatuí, os genótipos IAC 351, IAC 335, IAC 289 e Mochis; em Votuporanga, IAC 289, TUI"S" e SERI*3/BUC; em Ribeirão Preto e Mococa, o IAC 370. No conceito de genótipo ideal, o IAC 370 apresentou alta capacidade produtiva, foi responsivo à melhoria do ambiente e sensível às condições desfavoráveis do ambiente. Entre as doenças, a ferrugem-da-folha foi a de abrangência generalizada com maior incidência em Tatuí. Anahuac, IAC 287, CAL/CHKW//VEE"S" e IAC 370 foram os mais suscetíveis ao oídio. Os genótipos Anahuac, IAC 287, JCAM//EMU"S"/YACO"S", PFAU e IAC 339 foram os mais sensíveis à toxicidade por Al3+. Os genótipos Anahuac, IAC 24, IAC 287, IAC 289, IAC 334, PFAU, TUI"S", IAC 339, IAC 370 e IAC 351 apresentaram características de farinha com bom potencial para panificação.Environmental influence was evaluated in 20 wheat genotypes in relation to grain yield and quality, adaptability and disease reaction in regions where irrigation is necessary for good agronomical performance. The experiments were carried out at Tatuí, Votuporanga, Ribeirão Preto, and Mococa in the State of São Paulo, Brazil, during 1995-98. Genotypes were also evaluated for tolerance to aluminum toxicity in nutrient solutions and for industrial quality for bread production. The genotypes IAC 351, IAC 335, IAC 289 and Mochis presented high grain yield in Tatuí; IAC 289, TUI"S" and SERI*3/BUC in

  18. Genetic diversity of noroviruses in Brazil

    Directory of Open Access Journals (Sweden)

    Julia Monassa Fioretti

    2011-12-01

    Full Text Available Norovirus (NoV infections are a major cause of acute gastroenteritis outbreaks around the world. In Brazil, the surveillance system for acute diarrhoea does not include the diagnosis of NoV, precluding the ability to assess its impact on public health. The present study assessed the circulation of NoV genotypes in different Brazilian states by partial nucleotide sequencing analysis of the genomic region coding for the major capsid viral protein. NoV genogroup II genotype 4 (GII.4 was the prevalent (78% followed by GII.6, GII.7, GII.12, GII.16 and GII.17, demonstrating the great diversity of NoV genotypes circulating in Brazil. Thus, this paper highlights the importance of a virological surveillance system to detect and characterize emerging strains of NoV and their spreading potential.

  19. Interação genótipo x ambiente em cultivares de alface na região de Jaboticabal Interaction genotype x environment of lettuce cultivars in Jaboticabal, Brazil

    Directory of Open Access Journals (Sweden)

    Eduardo B. de Figueiredo

    2004-03-01

    Full Text Available Estudou-se a interação genótipo x ambiente de doze cultivares de alface, sendo quatro do grupo lisa (Babá de Verão, Karla, Nacional e Elisa, quatro do grupo crespa (Simpson, Hortência, Verônica e Grand Rapids e quatro do grupo americana (Laidy, Tainá, Lucy Brown e Raider. Os tratamentos foram constituídos pelo cultivo da alface em dez ambientes (casa de vegetação, túnel baixo de cultivo, túnel baixo com sombrite, agrotêxtil e campo, na presença e ausência de mulching. Foram utilizados dois períodos de cultivo, agosto a novembro de 2001 e março a junho de 2002 em Jaboticabal. Cada experimento (ambiente de cultivo foi conduzido utilizando-se o delineamento de blocos casualizados, com doze cultivares e três repetições. A análise de variância conjunta demonstrou valores de F significativos (pWe evaluated the interaction genotype x environment of twelve genotypes of lettuce (Babá de Verão, Karla, Nacional, Elisa, Simpson, Hortência, Verônica, Grand Rapids, Laidy, Tainá, Lucy Brown and Raider, cultivated under greenhouse, low tunnel, low tunnel covered with black plastic screen, floating row cover (light-weight woven and open field. All these treatments were conducted with and without mulching with ten treatments in total. The experiments were conducted in two different periods (August to November, 2001 and March to June, 2002. Each experiment (growth environment was conducted using randomized blocks and three replicates. The measured variables were dry matter, volume and number of leaves. The value of F was significant (p<0,01 for the interaction genotype x environment. For the planting period from August to November/2001, the best results were obtained with the butterhead cultivars (Babá de Verão, Karla, Nacional, Elisa, cultivated under greenhouse with mulching, low tunnel without mulching and field without mulching. All cultivars had the worst performance when cultivated under tunnel with black plastic screen with or

  20. Desempenho agronômico de genótipos de girassol no norte de Minas Gerais = Agronomic performance of sunflower genotypes in the north of Minas Gerais, Brazil.

    Directory of Open Access Journals (Sweden)

    Danúbia Aparecida Costa Nobre

    2012-08-01

    Full Text Available O girassol (Helianthus annuus L. encontra-se entre as quatro culturas de maior produção de óleo comestível no mundo, e destaca-se pela sua adaptação a diferentes condições edafoclimáticas. Objetivou-se com este estudo avaliar o desempenho agronômico de dez genótipos de girassol no norte de Minas Gerais. O estudo foi conduzido nas Fazendas Experimentais da Empresa de Pesquisa Agropecuária de Minas Gerais (Epamig, nos municípios de Jaíba, distrito de Mocambinho e NovaPorteirinha. Fez-se a avaliação do desempenho agronômico destes genótipos analisando as seguintes características: floração inicial, altura das plantas, diâmetro da haste, número de folhas,circunferência do capítulo, estande final e rendimento de grãos. Os dados coletados foram submetidos à análise de variância e ao teste de Scott-Knott a 5% de probabilidade. Houve variação em todos os parâmetros avaliados no comportamento agronômico dos genótipos, os quais apresentaram bom desempenho quando cultivados em Mocambinho e Nova Porteirinha, no norte de Minas Gerais. Os genótipos avaliados apresentam ciclo precoce, exceto o SYN 039A, com ciclo médio. Com a redução do ciclo da cultura, observa-se também redução na maioria das variáveis avaliadas.The sunflower (Helianthus annuus L. is one of the four cultures of the largest production of edible oil worldwide, and stands out for its adaptation to different edafoclimatic conditions. The objective of this study was to evaluate the agronomic performance of ten cultivars of sunflower in northern Minas Gerais. The study was conducted at the Experimental Farms of the Agricultural Research Company of Minas (Epamig in the municipalities of Jaíba, District of Mocambinho and Nova Porteirinha. The agronomic perfomance of these genotypes was evaluated by reviewing these characteristics: initial flowering,plant height, stem diameter, leaf number, circumference of the chapter, final stand, and yield. The data

  1. Carbon isotope fractionation for cotton genotype selection

    Directory of Open Access Journals (Sweden)

    Giovani Greigh de Brito

    2014-09-01

    Full Text Available The objective of this work was to evaluate the carbon isotope fractionation as a phenomic facility for cotton selection in contrasting environments and to assess its relationship with yield components. The experiments were carried out in a randomized block design, with four replicates, in the municipalities of Santa Helena de Goiás (SHGO and Montividiu (MONT, in the state of Goiás, Brazil. The analysis of carbon isotope discrimination (Δ was performed in 15 breeding lines and three cultivars. Subsequently, the root growth kinetic and root system architecture from the selected genotypes were determined. In both locations, Δ analyses were suitable to discriminate cotton genotypes. There was a positive correlation between Δ and seed-cotton yield in SHGO, where water deficit was more severe. In this site, the negative correlations found between Δ and fiber percentage indicate an integrative effect of gas exchange on Δ and its association with yield components. As for root robustness and growth kinetic, the GO 05 809 genotype performance contributes to sustain the highest values of Δ found in MONT, where edaphoclimatic conditions were more suitable for cotton. The use of Δ analysis as a phenomic facility can help to select cotton genotypes, in order to obtain plants with higher efficiency for gas exchange and water use.

  2. Modelos da distribuição temporal de chuvas intensas em Piracicaba, SP Time distribution models of intense rainfall in Piracicaba, SP, Brazil

    Directory of Open Access Journals (Sweden)

    Décio E. Cruciani

    2002-04-01

    Full Text Available O estudo da variação temporal de chuvas intensas é de grande importância na hidrologia, para a análise e previsão de eventos extremos, necessárias em projetos de controle de engenharia. Com esse objetivo, foram analisados dados de pluviogramas da cidade de Piracicaba, SP, do período de 1966 a 2000, para se determinar a distribuição temporal de chuvas intensas de 60 e de 120 min de duração. As chuvas de 60 min foram subdivididas em três intervalos iguais e sucessivos de 20 min cada um, enquanto as chuvas de 120 min foram subdivididas em quatro intervalos iguais e sucessivos de 30 min cada um. O modelo de distribuição da precipitação que predominou para as chuvas de 60 e 120 min, foi do tipo exponencial negativo, com 85,7 e 50,7% dos casos, respectivamente. Para as chuvas de 60 min, com altura pluviométrica média de 20,7 mm, a distribuição foi de 72,3, 21,4 e 6,2% do total precipitado, respectivamente, nos três intervalos sucessivos de 20 min. Para as chuvas de 120 min, com altura pluviométrica média de 33,3 mm, o resultado foi de 60,1, 25,2, 11,1 e 3,6%, respectivamente, nos quatro intervalos sucessivos de 30 min. O modelo de distribuição temporal dessas chuvas não foi modificado pelo total precipitado nem pela sua duração, nos intervalos em questão.Time distribution models of intense and short rains are very important in hydrology and for extreme predictions in engineering projects. With this purpose, rain data of Piracicaba, SP, Brazil, from 1966 to 2000 were analyzed to establish time distribution models of 60 and 120 min intense rains, during the rainy season from October through March. Time distribution models were assessed by three intervals of twenty minutes duration, for 60 min rains and by four intervals of thirty minutes duration for 120 min rains. The prevailing precipitation model for both, 60 and 120 min rains was a negative exponential distribution, in 85.7 and 50.7% of cases, respectively. For 60 min

  3. Histochemical studies on genetical control of hormonal enzyme inducibility in the mouse. III. Beta-glucuronidase distribution pattern of epididymis in different genotypes

    DEFF Research Database (Denmark)

    Blecher, S R; Kirkeby, S

    1979-01-01

    This article reports the application of Hayashi's histochemical technique for beta-glucuronidase to mouse epididymis. A methodological study, which established optimal conditions for demonstrating the enzyme in this organ, is reported. The distribution pattern of beta-glucuronidase is described...... and correlated with previous data for alpha-naphthyl acetate esterase. Differences between sites of granular and diffuse reaction product for these two enzymes are recorded. Possible interpretations of these findings in terms of intracellular localization of enzymes are discussed. Studies on different strains...

  4. AMMI analysis to evaluate the adaptability and phenotypic stability of sugarcane genotypes

    Directory of Open Access Journals (Sweden)

    Luís Cláudio Inácio da Silveira

    2013-02-01

    Full Text Available Sugarcane (Saccharum sp. is one of the most important crops in Brazil. The high demand for sugarcane-derived products has stimulated the expansion of sugarcane cultivation in recent years, exploring different environments. The adaptability and the phenotypic stability of sugarcane genotypes in the Minas Gerais state, Brazil, were evaluated based on the additive main effects and multiplicative interaction (AMMI method. We evaluated 15 genotypes (13 clones and two checks: RB867515 and RB72454 in nine environments. The average of two cuttings for the variable tons of pol per hectare (TPH measure was used to discriminate genotypes. Besides the check RB867515 (20.44 t ha-1, the genotype RB987935 showed a high average TPH (20.71 t ha-1, general adaptability and phenotypic stability, and should be suitable for cultivation in the target region. The AMMI method allowed for easy visual identification of superior genotypes for each set of environments.

  5. Distribution of stream macroalgal communities from the mid-western region of Paraná state, southern Brazil: importance of local scale variation

    Directory of Open Access Journals (Sweden)

    Ciro Cesar Zanini Branco

    2009-04-01

    Full Text Available Twenty-two stream segments, representing the diverse types of environments in the mid-western region of the Paraná State, southern Brazil, were sampled for occurrence of macroalgal communities from May to October in 2002. Twenty-seven macroalgal taxa were found, with Chlorophyta as the dominant algal group, followed by Cyanobacteria, Rhodophyta and Chrysophyta. The most widespread species was Phormidium retzii. Distribution was patchy, with species number per sampling sites ranging from zero to six and correlated positively with the abundance. On the other hand, no significant correlation was found among the species number and abundance with environmental variables. Results indicated wide and random variation among the streams. Thus, it seemed that the distribution of macroalgal communities in the study area responded more closely to the local-scale variation than the regional characteristics.Durante o período de maio a outubro de 2002, vinte e dois segmentos de riachos, representando os diversos tipos de ambientes da região centro-oeste do estado do Paraná, sul do Brasil, foram amostrados quanto à comunidade de macroalgas. Foram encontradas vinte e sete macroalgas. A divisão Chlorophyta foi o grupo algal predominante, seguido por Cyanobacteria, Rhodophyta e Chrysophyta. A espécie com melhor distribuição foi Phormidium retzii. Foi observado um padrão de distribuição em mosaico. O número de espécies por ponto de amostragem variou de zero a seis e correlacionou-se positivamente com abundância de espécies. Por outro lado, nenhuma outra correlação significativa foi encontrada entre número de espécies e abundância com as variáveis ambientais. Os resultados, suportados por dados de literatura, indicaram uma variação ampla e aleatória entre os riachos, sugerindo que a distribuição da comunidade de macroalgas na área de estudos parece responder mais fortemente a variações nas condições em escala local do que em escalas

  6. Calibration and parameterization of a semi-distributed hydrological model to support sub-daily ensemble flood forecasting; a watershed in southeast Brazil

    Science.gov (United States)

    de Almeida Bressiani, D.; Srinivasan, R.; Mendiondo, E. M.

    2013-12-01

    The use of distributed or semi-distributed models to represent the processes and dynamics of a watershed in the last few years has increased. These models are important tools to predict and forecast the hydrological responses of the watersheds, and they can subside disaster risk management and planning. However they usually have a lot of parameters, of which, due to the spatial and temporal variability of the processes, are not known, specially in developing countries; therefore a robust and sensible calibration is very important. This study conduced a sub-daily calibration and parameterization of the Soil & Water Assessment Tool (SWAT) for a 12,600 km2 watershed in southeast Brazil, and uses ensemble forecasts to evaluate if the model can be used as a tool for flood forecasting. The Piracicaba Watershed, in São Paulo State, is mainly rural, but has about 4 million of population in highly relevant urban areas, and three cities in the list of critical cities of the National Center for Natural Disasters Monitoring and Alerts. For calibration: the watershed was divided in areas with similar hydrological characteristics, for each of these areas one gauge station was chosen for calibration; this procedure was performed to evaluate the effectiveness of calibrating in fewer places, since areas with the same group of groundwater, soil, land use and slope characteristics should have similar parameters; making calibration a less time-consuming task. The sensibility analysis and calibration were performed on the software SWAT-CUP with the optimization algorithm: Sequential Uncertainly Fitting Version 2 (SUFI-2), which uses Latin hypercube sampling scheme in an iterative process. The performance of the models to evaluate the calibration and validation was done with: Nash-Sutcliffe efficiency coefficient (NSE), determination coefficient (r2), root mean square error (RMSE), and percent bias (PBIAS), with monthly average values of NSE around 0.70, r2 of 0.9, normalized RMSE of 0

  7. Dengue virus type 3 in Brazil: a phylogenetic perspective

    Directory of Open Access Journals (Sweden)

    Josélio Maria Galvão de Araújo

    2009-05-01

    Full Text Available Circulation of a new dengue virus (DENV-3 genotype was recently described in Brazil and Colombia, but the precise classification of this genotype has been controversial. Here we perform phylogenetic and nucleotide-distance analyses of the envelope gene, which support the subdivision of DENV-3 strains into five distinct genotypes (GI to GV and confirm the classification of the new South American genotype as GV. The extremely low genetic distances between Brazilian GV strains and the prototype Philippines/L11423 GV strain isolated in 1956 raise important questions regarding the origin of GV in South America.

  8. 宫颈上皮内瘤变组织中HPV感染基因型的比较研究%A comparative study of HPV infective genotypes distribution in cervical intraepithelial neoplasias

    Institute of Scientific and Technical Information of China (English)

    王懿; 梅静; 耿建祥; 阚延静; 范雪梅; 夏林; 王宏景; 龙秀荣; 赵雪

    2015-01-01

    目的:比较三级别宫颈上皮内瘤变(CIN)组织标本中人乳头瘤病毒(HPV)感染的基因型分布情况及其临床意义。方法采用聚合酶链式反应和基因芯片检测技术对20例宫颈正常组织、185例宫颈CIN1级、98例宫颈CIN2级和118例宫颈CIN3级患者宫颈组织标本进行23种HPV基因分型检测,并对受检者进行相关资料分析。结果20例宫颈正常组织检出 HPV感染者2例,总的HPV感染率为10.00%(2/20);185例宫颈CINⅠ组织检出 HPV感染者83例,总的 HPV感染率为44.87%(83/185);98例宫颈CINⅡ组织检出HPV感染者83例,总的HPV感染率为84.69%(83/98);118例宫颈CINⅢ组织检出 HPV感染者108例,总的 HPV感染率为91.53%(108/118)。结论聚合酶链式反应结合基因芯片技术可应用于宫颈上皮内瘤变组织标本的HPV基因分型检测,对我国女性宫颈病变 HPV感染基因型分布的研究及宫颈癌瘤的防治及其疫苗的研发具有十分重要的意义。%Objective To compare the distribution of 23 kinds of human papillomavirus genotypes in 3 grades tissues of cervical intraepithelial neoplasias(CIN1、2、3) in women and its clinical significance .Methods Polymerase chain reaction (PCR)and gene‐chips technology were utilized for the detection of 23 kinds of HPV genotypes in cervical tissue specimens from 20 cases of normal tissues ,185 cases of CIN1grades、98 cases of CIN2grades and 118 cases of CIN3grades .And related materials of all subjects were analyzed .Results In cervical normal tissues of 20 women ,total infection rate of HPV was 10 .00% (2/20 ) ,In 185 cases of CIN1grades ,total infection rate of HPV was 44 .87% (83/185) ,In 98 cases of CIN2grades ,total infection rate of HPV was 84 .69%(83/98)and In 118 cases of CIN3 grades ,total infection rate of HPV was 91 .53% (108/118) .Conclusion PCR and gene‐chip tech‐nology can detect single and multiple HPV genotypes

  9. Genotype and environment interaction on soybean yield in Mato Grosso State, Brazil Interação de genótipos com ambientes e produtividade da soja no Estado do Mato Grosso, Brasil

    Directory of Open Access Journals (Sweden)

    José Francisco Ferraz de Toledo

    2006-05-01

    Full Text Available The objective of this work was to investigate the genotype-environment interaction in Mato Grosso State, MT. The relative importance of locations, years, sowing dates and cultivars and their interactions was analyzed from data collected in regional yield trials performed in a randomized complete block design with four replications, from 1994-1995 through 1999-2000, in nine locations and two sowing dates. Individual and pooled analyses of variance over years and locations were performed. Complementary analyses of variances partitioned MT State in two main and five smaller regions, respectively: North and South of Cuiabá; and MT-South-A (Pedra Preta area, MT-South-B (Rondonópolis and Itiquira, MT-East (Primavera do Leste and Campo Verde, MT-Central (Nova Mutum, Lucas do Rio Verde and Sorriso and MT-Parecis (Campo Novo dos Parecis and Sapezal. Locations are relatively more important than years for yield testing soybeans in the MT State, therefore, investment should be made in increasing locations rather than years to improve experimental precision. Partitioning the MT State into regions has little impact on soybean yield testing results and, consequently, on the efficiency of the soybean breeding program in the State. Breeding genotypes with broad adaptation for the MT State is an efficient strategy for cultivar development.O objetivo deste trabalho foi investigar a interação de genótipos com ambientes no Estado do Mato Grosso, MT. Locais, anos, épocas de semeadura, cultivares e respectivas interações foram analisados em relação a dados de produtividade de grãos de ensaios regionais realizados em delineamento de blocos completos casualizados, conduzidos de 1994-1995 a 1999-2000, em nove locais e duas épocas de semeadura. Análises de variâncias individuais e conjuntas de anos e locais foram efetuadas. Análises complementares foram realizadas, dividindo o Estado em duas regiões principais e cinco regiões menores, respectivamente

  10. Avaliação de genótipos de tomateiro tipo Santa Cruz no período de ver��o, em Araguari, MG Evaluation of tomato genotypes (Santa Cruz type during the summer season, in Araguari, MG, Brazil

    Directory of Open Access Journals (Sweden)

    José Ricardo Peixoto

    1999-12-01

    Full Text Available O trabalho foi desenvolvido na fazenda Jordão (município de Araguari, MG, na época do verão (período das águas, com o objetivo de verificar o desempenho agronômico de genótipos de tomateiro tipo Santa Cruz. Utilizou-se o delineamento experimental blocos casualizados, com 16 tratamentos (genótipos e quatro repetições. A parcela experimental foi constituída por duas fileiras com 12 plantas cada, no espaçamento de 1,00 m entre linhas e 0,55 m entre plantas (1 planta/cova. Efetuaram-se 17 colheitas, sendo a primeira aos 69 dias após o transplante. Vários genótipos apresentaram um bom desempenho agronômico, principalmente Saladinha, Débora Plus, SM-16 e Atlas, podendo ser cultivados no período de verão. Apenas Saladinha e Atlas ultrapassaram 140 g de peso médio, destacando-se também em frutos do tipo extra AA. Observou-se uma correlação significativa e negativa com r = -0,52 e -0,54 na primeira avaliação, e r = -0,55 e -0,45 na segunda avaliação para a produção total e produção comercial, respectivamente, em relação à incidência de geminivírus nos diferentes genótipos. Os híbridos Saladinha e SM-16 apresentaram o menor número de plantas viróticas, enquanto Santa Clara Importada, Santa Clara, Jumbo AG-592 e IAC Santa Clara, apresentaram o maior número.A field experiment was carried out in the Jordão farm in Araguari, MG, Brazil, during the summer season, to evaluate the agronomic potential of genotypes (Santa Cruz type of tomato. A randomized block design was used with 16 treatments (genotypes and four replicates. The experimental plot was made up of two lines (1.00 m between lines and 0.55 m between plants with 12 plants each (1 plant/pit. Seventeen harvests were made, starting 69 days after transplanting. Several genotypes as Saladinha, Débora Plus, SM-16 and Atlas had a good agronomic performance, being recommended to be cultivated in the region, in the summer season. Classified as AA type, the Saladinha

  11. 国内南方人群遗传性共济失调不同基因亚型的分布状况%Distribution of different genotypes of hereditary ataxia in south China

    Institute of Scientific and Technical Information of China (English)

    谢秋幼; 梁秀龄; 李洵桦

    2006-01-01

    BACKGROUND: It is of great importance to study the genotype distribution of hereditary ataxia in understanding its epidemiologic rule and pathogenetic pathway.OBJECTIVE: To analyze the distribution of different genotype of hereditary ataxia in south China.DESIGN: A case-control observation.SETTING: Department of Neurology, the First Affiliated Hospital of Sun Yat-sen University.PARTICIPANTS: Forty-three patients (26 males and 17 females) with hereditary ataxia from 36 families and 38 patients with sporadic hereditary ataxia (24 males and 14 females) were selected from the Outpatient Clinic of Neurogenetics, Department of Neurology, the First Affiliated Hospital of Sun Yat-sen University between September 1998 and September 2002. At the same time, 60 healthy individuals from the patients' families and 44randomly-selected healthy physical examinees were taken as controls. All the participants were enrolled voluntarily.METHODS: The fragments of trinucleotide repeats at different sites of mutant genes were amplified with polymerase chain reaction (PCR), and then the lengths were calculated with polyacrylamide gel electrophoresis and imaging analytical software. The repeated numbers of trinucleotide repeats in all the normal and abnormal amplified alleles were calculated respectively.MAIN OUTCOME MEASURES: Different genotype distribution in patients with hereditary ataxia.RESULTS: All the subjects were involved in the analysis of results. Of the detected patients with hereditary ataxia, the Machado-Joseph disease/spinocerebellar ataxia (SCA) 3 was the most common type of autosomal dominant SCA in South China, which was 42.0%, and was followed by SCA2 (7.4%), SCA1 (4.9%), SCA7 (3.7%), SCA6 (2.5%), SCA12 (1.2%).No patient was detected to have SCA8 SCA 10, SCA 17 dentatorubropallidoluysian atrophy (DRPLA) and Friedreich ataxia (FRDA).CONCLUSION: Autosomal dominant SCA3 is the most familiar genotype in South China. Clinical detection of hereditary ataxia should be done

  12. Molecular epidemiology and distribution of serotypes, genotypes, and antibiotic resistance genes of Streptococcus agalactiae clinical isolates from Guelma, Algeria and Marseille, France.

    Science.gov (United States)

    Bergal, A; Loucif, L; Benouareth, D E; Bentorki, A A; Abat, C; Rolain, J-M

    2015-12-01

    This study describes, for the first time, the genetic and phenotypic diversity among 93 Streptococcus agalactiae (group B Streptococcus, GBS) isolates collected from Guelma, Algeria and Marseille, France. All strains were identified by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). The molecular support of antibiotic resistance and serotyping were investigated by polymerase chain reaction (PCR). The phylogenetic lineage of each GBS isolate was determined by multilocus sequence typing (MLST) and grouped into clonal complexes (CCs) using eBURST. The isolates represented 37 sequence types (STs), 16 of which were novel, grouped into five CCs, and belonging to seven serotypes. Serotype V was the most prevalent serotype in our collection (44.1%). GBS isolates of each serotype were distributed among multiple CCs, including cps III/CC19, cps V/CC1, cps Ia/CC23, cps II/CC10, and cps III/CC17. All isolates presented susceptibility to penicillin, whereas resistance to erythromycin was detected in 40% and tetracycline in 82.2% of isolates. Of the 37 erythromycin-resistant isolates, 75.7% showed the macrolide-lincosamide-streptogramin B (MLSB)-resistant phenotype and 24.3% exhibited the macrolide (M)-resistant phenotype. Constitutive MLSB resistance (46%) mediated by the ermB gene was significantly associated with the Guelma isolates, whereas the M resistance phenotype (24.3%) mediated by the mefA/E gene dominated among the Marseille isolates and belonged to ST-23. Tetracycline resistance was predominantly due to tetM, which was detected alone (95.1%) or associated with tetO (3.7%). These results provide epidemiological data in these regions that establish a basis for monitoring increased resistance to erythromycin and also provide insight into correlations among clones, serotypes, and resistance genes. PMID:26415872

  13. Maternal Gene Polymorphisms Involved in Folate Metabolism as Risk Factors for Down Syndrome Offspring in Southern Brazil

    Directory of Open Access Journals (Sweden)

    Ana Paula Carneiro Brandalize

    2010-01-01

    Full Text Available This study aimed to investigate the role of maternal polymorphisms, as well as their risk genotypes combinations of MTR A2756G, MTRR A66G, CBS 844ins68, and RFC A80G, involved in folate/homocysteine metabolism, as possible risk factors for Down syndrome (DS in Southern Brazil. A case-control study was conducted with 239~mothers of DS children and 197 control mothers. The investigation of polymorphisms was performed by PCR and PCR-RFLP. The distribution of genotypic variants was similar in both groups when they were analyzed separately. An investigation of combined risk genotypes showed that the risk of having a DS child for one, two or three risk genotypes was 6.23, 6.96 and 5.84 (95%CI 1.48–26.26; 1.69–28.66; 1.37–24.86, respectively. The combined MTRR 66G and MTHFR 677T alleles were significantly more common among mothers of children with DS than among control mothers (OR 1.55; IC 95% 1.03–2.35. The results show that individual polymorphisms studied in this work are not associated with DS; however, the effects of the combined risk genotypes among MTR, MTRR, CBS and RFC genes are considered maternal risk factors for DS offspring in our population.

  14. Topologia do risco de acidentes do trabalho em Piracicaba, SP Spatial distribution of risks for work-related injuries in a city of Southeastern e Brazil

    Directory of Open Access Journals (Sweden)

    Lia Thieme Oikawa Zangirolani

    2008-04-01

    Full Text Available OBJETIVO: Analisar a distribuição espacial do risco de acidente do trabalho controlado por variáveis nutricionais e outras co-variáveis. MÉTODOS: Estudo caso-controle espacial de base hospitalar, tendo como variável de interesse a localização espacial dos acidentes do trabalho. Foram amostrados 794 trabalhadores, no período de maio a outubro de 2004. Os critérios de inclusão para casos (N=263 foram: ser trabalhador acidentado do trabalho, morador de Piracicaba, com idade entre 15 e 60 anos, e atendido em centro de ortopedia e traumatologia. Os controles (N=531 tiveram o mesmo critério de idade e residência na cidade, exceto que o acidente não era do trabalho, tendo sido considerandos também trabalhadores acompanhantes dos casos. A distribuição espacial da estimativa baseou-se no ajuste do modelo aditivo generalizado, tendo as coordenadas geográficas dos casos e controles como componente espacial não linear e as demais co-variáveis como componente linear. RESULTADOS: A variação da estimativa do risco espacial de acidentes do trabalho, controlada por sexo (OR=1,87; pOBJECTIVE: To assess spatial distribution of risks for work-related injuries controlled for nutritional variables and other covariables. METHODS: Hospital-based spatial case-control study with work-related injuries spatial distribution as the main variable of interest. A total of 794 workers were selected between May and October 2004. Inclusion criteria for cases (N=263 were: worker with work-related injury; living in Piracicaba (Southeastern Brazil; age between 15 and 60 years old; and cared at an orthopedics and trauma center. Controls (N=531 met the same criteria for age and residence, but had non-work-related injuries and workers accompanying cases were included as well. Spatial distribution was estimated by adjusting a generalized additive model with geographical coordinates of cases and controls as spatial non-linear component and the remaining covariables

  15. Evolution of garnet distribution, shape and composition in high-grade pelitic migmatites of Salvador da Bahia, Brazil: insights from LA-ICP-MS trace element mapping

    Science.gov (United States)

    Goncalves, Philippe; Raimondo, Tom; Santos de Souza, Jailma

    2016-04-01

    Garnet is a widely used mineral in metamorphic petrology and more particularly for thermobarometric modelling to reconstruct the P-T-t evolution of Earth's crust. This is due to its ubiquity in high grade rocks (T > 450°C), its occurrence in many assemblages of interest for thermobarometry, and mostly to its ability to preserve chemical zoning. Two types of zoning are distinguished: growth and diffusion zoning. Growth zoning reflects crystallisation coeval with changes in P-T conditions or bulk composition. This type of zoning is therefore particularly useful to unravel the P-T evolution of open systems and determine the growth mechanisms involved. However, growth zoning in major elements is commonly altered by processes such as volume diffusion, which is particularly efficient at high temperatures and for elements like Fe or Mg that have high diffusion coefficients. In such cases, information that relates to the environmental conditions of garnet growth is either totally or partially obliterated. To minimise the impact of this process on growth zoning and retain useful information, trace elements are more appropriate because of their lower diffusion coefficients compared to major elements. In this study, the distribution of trace elements in garnet has been imaged using an emerging LA-ICP-MS mapping technique. This is achieved by rastering of the focused laser beam in linear transects, which are then stitched together by post-acquisition processing to form a quantified or semi-quantified image of the trace element distribution, with excellent detection limits (ppb) over a wide isotopic range (7Li to 238U) and minimal sample preparation required. This technique has been applied to high-grade pelitic gneisses and migmatites from the Paleoproterozoic Itabuna-Salvador-Curaça belt (adjacent to the Farol da Bara, Salvador da Bahia, Brazil). Structurally, it is located in a steeply-dipping high strain zone that may have played a major role in the segregation and

  16. The HBV E Genotype Discover in Dai Nationality in Xishuangbanna, Yunnan Province

    Institute of Scientific and Technical Information of China (English)

    Hai-ping ZHAO; Yuan-ying SHEN; Ru SHEN; Yuan-yi WANG; Mei-ya FU

    2009-01-01

    To investigate the distribution of Hepatitis B virus (HBV) genotypes among the population of Dai nationality in Xishuangbanna, Yurman Province HBV genotypes of the Serum samples were tested by PCR-RFLP. This is the first time to discover the B+E genotypes in China. This finding provides new information for understanding the distribution of HBV genotype in China and a provides a basis for establishing a Chinese gene bank.

  17. Genotipagem de polimorfismos associados com sistemas de macho-esterilidade em acessos de cebola adaptados ao Brasil Genotyping of polymorphisms associated with male-sterility systems in onion accessions adapted for cultivation in Brazil

    Directory of Open Access Journals (Sweden)

    Carlos Francisco Ragassi

    2012-09-01

    lines for the Brazilian onion hybrid breeding programs. In the present work, polymerase chain reaction (PCR assays were conducted using mitochondria-derived markers in order to characterize the cytoplasm type present in different morpho-agronomic onion types cultivated in Brazil. The S cytoplasm appeared to be the most frequent one among the 66 evaluated accessions with a frequency of 56%. The T male-sterility-inducing cytoplasm was observed in 25.8% of the accessions. Of the samples, 18.2% displayed exclusively the cytoplasm N. This information could be useful to guide the onion breeding programs in the selection of parental lines aiming to develop hybrid cultivars adapted to tropical and subtropical areas of the world.

  18. Angiostrongylus costaricensis: first record of its occurrence in the State of Espirito Santo, Brazil, and a review of its geographic distribution Angiostrongylus costaricensis: primeiro relato de sua ocorrência no estado do Espírito Santo, Brasil, e revisão de sua distribuição geográfica

    OpenAIRE

    Gil Patrus M. Pena; José de Souza Andrade Filho; Samuel Curvelo de Assis

    1995-01-01

    Abdominal angiostrongyliasis is a parasitic disease caused by Angiostrongylus costaricensis, a metastrongylid nematode with wide geographic distribution, occurring from the United States to Argentina. In Brazil, the disease has been reported from the States of Rio Grande do Sul, Santa Catarina, Paraná, São Paulo, Federal District of Brasilia and Minas Gerais. We report here a case of abdominal angiostrongyliasis in a 9-year-old girl, from Itatiba, State of Espirito Santo, Brazil, submitted to...

  19. Geographic distribution's ampliation of Chiroderma doriae Thomas (Mammalia, Chiroptera) in Brazil Ampliação da distribuição geográfica de Chiroderma doriae Thomas (Mammalia, Chiroptera) no Brasil

    OpenAIRE

    Marcelo O Bordignon

    2005-01-01

    The geographic distribution ampliation of Chiroderma doriae Thomas, 1891 is related in this note with a new record from Corumba City, in pantanal ecossistem of Mato Grosso do Sul State. This record extend the geographic range of C. doriae over 630 km westward in Brazil from literature last reccord.Nesta nota é relatada a ampliação da distribuição geográfica de Chiroderma doriae Thomas, 1891 com um registro inédito na cidade de Corumbá, na região do Pantanal do Estado de Mato Grosso do Sul. Es...

  20. Spatial distribution of the Ocypode quadrata (Crustacea: Ocypodidae) along estuarine environments in the Paranaguá Bay Complex, southern Brazil Distribuição espacial de Ocypode quadrata (Crustacea: Ocypodidae) ao longo de ambientes estuarinos no sul do Brasil

    OpenAIRE

    Leonardo C. da Rosa; Carlos A. Borzone

    2008-01-01

    This study examines the spatial distribution of the ghost crabs, Ocypode quadrata Fabricius, 1787, in thirteen estuarine sandy beaches located along two main axes of the Paranaguá Bay Estuarine Complex, southern Brazil. Burrow densities of ghost crabs were measured at three beach levels established around the high tide mark during the summer and winter of 2005. All beaches showed a steep beach face slope (2.6 to 8.3º) with sediment composition varying from well sorted fine sand to very poorly...

  1. Distribution of serotypes of Streptococcus pneumoniae isolated from invasive infections over a 16-year period in the greater São Paulo area, Brazil.

    OpenAIRE

    Brandileone, M. C.; Vieira, V S; Zanella, R C; I.M. Landgraf; Melles, C E; Taunay, A; J. C. Moraes; Austrian, R.

    1995-01-01

    Capsular types of pneumococci from normally sterile body sites of 1,622 patients in Brazil were analyzed. Of 1,477 isolates from cerebrospinal fluid, 76.1% were of types represented in the currently available pneumococcal vaccine. The importance of age, time, and place in determining the optimal formulation of pneumococcal vaccine is considered.

  2. Survey and competition assay data suggest species-specific difference in host/niche adaptation influence the distribution of Fusarium graminearum species complex pathogens in Brazil

    Science.gov (United States)

    The Fusarium graminearum species complex (FGSC) comprises at least 16 toxigenic species of economic concern to cereal crops. In Brazil, six species of the FGSC have been identified, but their frequencies vary according to the host species. Although F. graminearum (Fgra) is dominant in wheat (>90%) a...

  3. Trigo duro: comportamento de genótipos no estado de São Paulo Durum wheat: evaluation of genotypes for the state of São Paulo, Brazil

    Directory of Open Access Journals (Sweden)

    Carlos Eduardo de Oliveira Camargo

    1995-01-01

    medium stature. These lines presented good grain yield in soils with low acidity. However, they did not differ in yield from the wheat cultivar IAC-60, the most cultivated actually in the State of São Paulo and from the triticale Álamos. The durum wheat line 19 (Mindum/Kingfisher "S"//Sandpiper showed at the same time immunity to the causal agents of stem (with exception of line 21 and leaf rusts and moderate resistance to the causal agent of powdery mildew. The cultivars Álamos (triticale and IAC-29 (wheat exhibited immunity to the causal agents of powdery mildew. All considered genotypes were susceptible to the leaf spots patogens, with exception of the durum wheat line 6 (Dackiye/Gerardo Vezio 394 which was moderately resistant.

  4. 308例宫颈瘤组织中HPV感染基因型分布的对比研究%A comparative study of HPV infection genotypes distribution in 308 cases of cervical intraepithelial neoplasia tissues

    Institute of Scientific and Technical Information of China (English)

    朱晓珏; 耿建祥

    2014-01-01

    目的:比较宫颈上皮内瘤病变(cervical intraepithelial neaplasia,CIN)组织标本中人乳头瘤病毒(human papillomavir-us,HPV)感染的基因型分布情况及其临床意义。方法采用聚合酶链式反应和基因芯片检测技术对53例 CINⅠ、67例 CINⅡ和188例 CINⅢ病变组织标本进行23种 HPV 基因分型检测,并对受检者进行相关资料分析。结果53例 CINⅠ患者检出 HPV感染者29例,总的 HPV 感染率为54.72%(29/53);67例 CINⅡ患者检出 HPV 感染者51例,总的 HPV 感染率为76.12%(51/67);188例 CINⅢ患者检出 HPV 感染者175例,总的 HPV 感染率为93.09%(175/188)。结论 CINⅠ、CINⅡ和 CINⅢ组织中总的 HPV 感染率存在着明显的差异性,聚合酶链式反应与基因芯片检测技术可应用于宫颈组织标本检测,一次可检测23种HPV 基因型别,对我国女性宫颈 HPV 感染的分子流行病学调查、宫颈癌瘤的防治及其疫苗研究具有非常重要的意义。%Objective To compare the distribution situation of human papillomavirus(HPV)genotypes in the tissue specimens of cervical intraepithelial neoplasia(CINⅠ,CINⅡ and CINⅢ)and its clinical significance.Methods The polymerase chain reaction (PCR)and gene-chips technology were utilized for detecting 23 kinds of HPV genotypes in the cervical tissue specimens from 53 ca-ses of CINⅠ,67 cases of CINⅡ and 188 cases of CINⅢ.And the related data of all subjects were analyzed.Results Among 53 ca-ses of CINⅠ,29 cases of HPV infection were detected with the total HPV infection rate of 54.72%9;among 67 cases of CINⅡ,52 cases of HPV infection were detected with the total HPV infection rate of 76.12%;among 188 cases of CINⅢ,175 cases of HPV infection were detected with the total HPV infection rate of 93.09%.Conclusion The significant difference of the total HPV infec-tion rate exists in the tissues of CINⅠ,CINⅡ and CINⅢ.PCR and the gene

  5. 大连地区不同年龄段女性人乳头瘤病毒感染及基因亚型分布调查%Research on HPV infection and its genotypes distribution in female at different ages in Dalian area

    Institute of Scientific and Technical Information of China (English)

    杨真; 侯剑; 李小欢; 袁小林

    2013-01-01

    OBJECTIVE To explore the prevalence of human papillomavirus (HPV) infection and its genotypes distribution in female at different ages in Dalian area and to provide theoretical basis for screening and preventing cervical cancer.METHODS The infectious status of HPV in 1341 females in Dalian area was examined with gene chip technique and various genotypes of HPV were compared and analyzed.The positive rate,multiple infection,gene type and genotypes distribution were calculated respectively at different ages.RESULTS 280 of 1 341 cases were positive for HPV (20.88%).Single,double,triplex and quadruplex genotypes of HPV accounted for 77.50% (217/280),14.64% (41/280),6.07% (17/280) and 1.79% (5/280),respectively.Low-risk genotypes,high-risk genotypes and low-risk combined with high-risk genotypes accounted for 20.00% (56/280),70.00% (196/280) and 10.00% (28/280),respectively.Twenty genotypes were detected,including low-risk genotypes (HPV-6,11,42,43) and high-risk genotypes (HPV-16,18,31,33,35,45,51,52,53,56,58,59,66,68,73,83),and the predominant genotypes were HPV43 and HPV16 respectively in low-risk and high-risk genotypes.CONCLUSION The determination of HPV genotypes was important for early discovery,diagnosis and treatment of cervical cancer.%目的 探讨大连地区不同年龄段女性人乳头瘤病毒(HPV)的感染及基因亚型分布情况,为宫颈癌的筛查及防治提供理论依据.方法 采用PCR体外扩增和DNA反向点杂交相结合的DNA芯片技术,对大连地区1 341例成年女性HPV的感染情况进行定性检测并加以分型,按不同年龄分组,分别统计HPV感染阳性率、多重感染、基因型别及亚型分布情况.结果 1341例女性标本中,共检出HPV阳性标本280例,阳性率为20.88%;单一感染217例(占77.50%),双重感染41例(占14.64%),三重感染17例(占6.07%),四重感染5例(占1.79%);单纯低危型感染56例(占20.00%),单纯高危型感染196例(占70.00%),低

  6. Microsatellite genotyping from faeces of Lontra longicaudis from southern Brazil Genotipagem através de microsatélites extraídos de amostras fecais em Lontra longicaudis do sul do Brasil

    Directory of Open Access Journals (Sweden)

    Laura I. Weber

    2009-03-01

    Full Text Available A genetic study of the neotropical river otter Lontra longicaudis (Olfers, 1818, which has an unknown conservation status, was carried out at the Taim Ecological Station and the margins of the Vargas stream, Rio Grande do Sul, southern Brazil. Faecal samples were collected, and DNA was extracted using a silica-guanidine method. Five microsatellite loci were amplified using PCR with heterologous primers previously described for Lutra lutra (Linnaeus, 1758. Sixteen faecal samples out of 29 from Taim and 11 out of 14 from Vargas stream margins contained enough DNA for genetic analysis. A total of 49 different alleles were found at both localities, from which 18 were exclusively found in individuals from Taim and 17 were exclusives from Vargas individuals. The most common allele was the same at both locations for three loci (Lut715, Lut733, and Lut818. A high level of genetic diversity was found at both sites (NeTaim=4.1, HoTaim=0.299, HeTaim=0.681; NeVargas=4.9, HoVargas=0.355, HeVargas=0.724, being higher at the Vargas stream site. A high and significant level of heterozygote deficiency was observed at most loci according to the χ2 test. The homogeneity χ2 test (PA lontra neotropical de rio Lontra longicaudis (Olfers, 1818, cujo estado de conservação é ainda desconhecido, foi estudada geneticamente na Estação Ecológica do Taim e nas margens do arroio Vargas, RS, sul do Brasil. Amostras de fezes foram coletadas e o DNA foi extraído por um método de sílica-guanidina. Cinco locos de microsatélites foram amplificados por PCR utilizando primers heterólogos previamente descritos para Lutra lutra (Linnaeus, 1758. Dezesseis amostras de fezes de um total de 29 coletadas no Taim e onze das 14 obtidas no arroio Vargas contiveram DNA suficiente para prosseguir com a analise genética. Um total de 49 alelos foram obtidos, dos quais 18 foram exclusivos de indivíduos do Taim e 17 exclusivos dos indivíduos do arroio Vargas. Em três locos (Lut715

  7. Distribution of HPV infective genotypes in condyloma acuminatum tissues of cervix%子宫颈尖锐湿疣组织中HPV感染基因型别分布

    Institute of Scientific and Technical Information of China (English)

    蔡为民; 阚延静; 耿建祥; 龙秀荣; 梅静; 王宏景; 夏林; 王旭波; 赵雪

    2015-01-01

    Purpose To compare the distribution of 23 kinds of human papillomavirus ( HPV) genotypes in tissues of condyloma acu-minata ( CA) of cervix in 120 women and its clinical significance. Methods Polymerase chain reaction ( PCR) and gene-chips tech-nology were utilized for the detection of 23 kinds of HPV genotypes in tissue specimens from 120 cases of CA in cervix and related ma-terials of all subjects were conducted and analyzed. Results There were 115 positive cases in 120 women with CA in cervix and the rate of total HPV infection was 95. 83% (115/120). The rate of single type was 70. 83% (85/120) and multiple types was 25. 00%(30/120). The predominant type of single infection was HPV11 and the infective rate was 45. 00% (54/120), followed by HPV6 (22. 50%, 27/120). Otherwise, the predominant type of multiple infections was HPV6+11 with the infective rate of 20. 00% (6/30), and HPV11+16 infection accounted for 10. 00% (3/30). Conclusions HPV11, 6, 6+11 and 11+16 are the main genotypes in the pathogenesis of CA in cervix in 120 women. PCR and gene-chip technology can detect single and multiple HPV genotyping in tis-sues of CA in cervix with high sensitivity and specificity. Detection of HPV genotypes could be used to understand the prevalence situa-tion of HPV infection in tissues of CA and tumors of cervix and further to provide references for the research and development of HPV vaccine in women.%目的:探讨子宫颈尖锐湿疣( condyloma acuminatum, CA)组织中人乳头瘤病毒( human papillomavirus, HPV)感染的基因型别分布及临床意义。方法采用PCR和基因芯片技术对120例子宫颈CA组织进行23种HPV基因分型检测,并对患者的临床病理资料进行分析。结果120例子宫颈CA组织中检出HPV阳性感染115例,HPV感染率为95.83%(115/120)。其中一重HPV感染者85例,感染率为70.83%(85/120),多重HPV感染者30例,感染率为25.00%(30/120)。一重HPV感染中HPV11型阳性检出率为45.00%(54/120),是

  8. Avaliação agronômica de genótipos de bananeiras em condições subtropicais, Vale do Ribeira, São Paulo - Brasil Agronomic evaluation of banana plants genotypes in subtropical conditions, Vale do Ribeira, São Paulo - Brazil

    Directory of Open Access Journals (Sweden)

    Edson Shigueaki Nomura

    2013-03-01

    diseases, appropriate size and resistance to drought and cold, there are few cultivars with agronomic potential to be used commercially. Aiming to evaluate the vegetative development of banana genotypes at Vale do Ribeira (Brazil conditions the following materials were tested, separated into two genomic groups: AAAA (Bucaneiro, FHIA 02 and FHIA 17, and AAAB, subdivided according to the cultivar used in the breeding: Prata (BRS Garantida, FHIA 18, BRS FHIA Maravilha, BRS Platina and PA94-01; Pacovan (BRS Japira, BRS Pacovan Ken, PV79-34, PV94-01 and BRS Vitória; and Yangambi n.2 (BRS Tropical, BRS Princesa and YB42-03. The cultivars Grande Naine (AAA, Pacovan (AAB, Prata anã (AAB and Yangambi (AAA present development, production and sensory characteristics similar to the evaluated genotypes, so they were used to compare to standards. During two cycles it was evaluated: height, pseudo stem diameter, number of active leaves (on blooming and in the harvest, the interval between planting and blooming and the interval between planting and harvest (days. It was calculated confidence intervals in different groups and types of bananas. According to the obtained data it can be concluded that genotypes from AAAB group with 'Paconvan' genitor cultivar are not suitable for cultivation in the region due to the plants height. The genotypes that have potential to be cultivated in Vale do Ribeira region are: AAAA (FHIA 02 and FHIA 17, AAAB group with 'Prata' genitor cultivar (FHIA 18, BRS Garantida and PA94-01 and with 'Yangambi n.2' genitor cultivar (BRS Tropical, BRS Princesa and Yangambi.

  9. Detecção e variabilidade de Plasmopara halstedii no Brasil e avaliação da resistência de genótipos de girassol ao míldio Detection and variability of Plasmopara halstedii in Brazil and resistance of sunflower genotypes to downy mildew

    Directory of Open Access Journals (Sweden)

    Regina Maria Villas Bôas de Campos Leite

    2007-12-01

    Full Text Available Este trabalho foi conduzido com o objetivo de identificar a raça fisiológica de Plasmopara halstedii que ocorreu em plantas de girassol coletadas no campo experimental da Embrapa Soja, Londrina, PR, em 1998, 2001 e 2002 e avaliar a reação de genótipos de girassol ao míldio. Plântulas de girassol das diferenciadoras de raças e das cultivares foram inoculadas com suspensão de zoosporângios do patógeno e foram plantadas em caixas contendo areia autoclavada. As plântulas foram mantidas em câmara climatizada, com temperatura controlada em 21ºC, por 11 dias. Em seguida, as plantas foram aspergidas intensamente com água destilada, cobertas com saco plástico e mantidas no escuro, a 18ºC. No dia seguinte, foi observada a presença de esporulação nos cotilédones. As plantas que apresentaram esporulação foram consideradas suscetíveis e as sem esporulação foram resistentes. O resultado indicou tratar-se da raça 330 (antiga raça 7 americana, nas três ocasiões. Os genótipos de girassol Embrapa 122, BRS 191 e as cultivares de girassol ornamental BRS Capri M, BRS Encanto M, BRS Oásis, BRS Paixão M, BRS Pesqueiro M, BRS Refúgio M, BRS Saudade M e BRS Saudade U e seus respectivos parentais foram suscetíveis a P. halstedii raça 330. Os genótipos AGROBEL 910, AGROBEL 920, AGROBEL 960, AGROBEL 965, C11, EXP38, M734, M742 e RUMBOSOL 91 foram resistentes à raça 330 do patógeno e podem ser indicados aos agricultores para uso em regiões de risco de ocorrência da doença.This research was carried out for identifying the physiological race of Plasmopara halstedii occurring in sunflower at the experimental field of Embrapa Soybean, Londrina, PR, Brazil, in 1998, 2001 and 2002 by evaluating the reaction of sunflower genotypes inoculated with downy mildew pathogen. Sunflower seedlings of the differentials set to identify races and of the cultivars were inoculated by immersion in zoosporangia suspension and were grown in autoclaved

  10. Dalian coastal area female HPV infection and genotyping distribution investigation%大连地区女性人乳头瘤病毒感染状况及基因分型分布情况调查

    Institute of Scientific and Technical Information of China (English)

    钱慧珍; 范晓明; 张君; 杨美娇

    2014-01-01

    Objective Research Dalian region of human papillomavirus infection rate,genotype and age distribution.Determine the area advantage type and high risk population,at the same time to verify that the HPV gene detection method and method of cytological examination of differences.Methods The real-time fluorescence quantitative PCR and gene chip hybridization typing technique in 4177 cases of Clinic Women for HPV genotyping assay.Analysis of genotype HPV infection distribution characteristics,calculation of the age in women at high risk of HPV infection rate.On HPV virus positive patients for cytology test,analysis of different types of infection patient cytology positive rate difference.Results Dalian region women's HPV infection rate was 17.09% (711/4160),hr-HPV infection rate was 13.73% (572/4160),low risk type HPV infection rate was 3.36% (140/4160),the highest infection rate in genotype HPV16 (13.50%),followed by 58,56,33,35,and 68 type.The positive rate of hr-HPV between women of different age groups showed significant difference (P< 0.05),the positive rate was the highest in < 30 years old.High risk group of mixed infection of cytology positive rate was higher than that of single hr-HPV infection group and the low risk of infection group.Conclusions Local people infected with HPV advantage of type HPV16.The positive rate of hr-HPV was associated with age,< 30 years is the peak of infection of high risk hr-HPV.The mixed infection of cytologically positive rate than other types of infection.%目的 研究大连地区人乳头瘤病毒(HPV)感染率、基因分型和年龄分布特征,确定本地区的优势型别及高风险人群,同时验证HPV基因检测方法与细胞学检测方法的差异.方法 采用实时荧光定量PCR和基因芯片杂交分型技术对4177例门诊妇女进行HPV基因分型检测.分析HPV基因型感染分布特点,计算各年龄段妇女高危型HPV的感染率.对HPV病毒阳性患者进行

  11. Measurements of experimental precision for trials with cowpea (Vigna unguiculata L. Walp.) genotypes.

    Science.gov (United States)

    Teodoro, P E; Torres, F E; Santos, A D; Corrêa, A M; Nascimento, M; Barroso, L M A; Ceccon, G

    2016-01-01

    The aim of this study was to evaluate the suitability of statistics as experimental precision degree measures for trials with cowpea (Vigna unguiculata L. Walp.) genotypes. Cowpea genotype yields were evaluated in 29 trials conducted in Brazil between 2005 and 2012. The genotypes were evaluated with a randomized block design with four replications. Ten statistics that were estimated for each trial were compared using descriptive statistics, Pearson correlations, and path analysis. According to the class limits established, selective accuracy and F-test values for genotype, heritability, and the coefficient of determination adequately estimated the degree of experimental precision. Using these statistics, 86.21% of the trials had adequate experimental precision. Selective accuracy and the F-test values for genotype, heritability, and the coefficient of determination were directly related to each other, and were more suitable than the coefficient of variation and the least significant difference (by the Tukey test) to evaluate experimental precision in trials with cowpea genotypes. PMID:27173351

  12. Occurrence and distribution of the exotic lizard Hemidactylus mabouia Moreau de Jonnès, 1818 in Ilha Grande, RJ, Brazil.

    Science.gov (United States)

    Rocha, C F D; Bergallo, H G

    2011-05-01

    The gekkonid lizard Hemidactylus mabouia is an exotic species in Brazil and is found in different ecosystems. This species was recorded at Ilha Grande, RJ, one of the largest insular remains of the Atlantic Rainforest in Brazil. In this study, the occurrence of H. mabouia was determined throughout the island, including the rain forest, restinga and anthropic environments. We used the active search method in points along 19 trails that surround Ilha Grande. At each regular interval of 100 m, we searched for the presence of H. mabouia. The species was recorded in a total of 100 points among the 719 sampled and, in all cases, the occurrence of the lizard corresponded to points located in anthropic or perianthropic areas. As most of Ilha Grande is covered by dense tropical rain forest, we believe this has restricted the invasion of H. mabouia in natural environments within the island. PMID:21755162

  13. Offshore distribution patterns of the cyanobacterium Trichodesmium erythraeum ehrenberg and associated phyto- and bacterioplankton in the southern Atlantic coast (Paraná, Brazil)

    OpenAIRE

    Adriana Siqueira; Hedda Elisabeth Kolm; Frederico Pereira Brandini

    2006-01-01

    Studies were carried out on Thrichodesmium erythraeum occurring on the inner shelf in the state of Paraná, Brazil. Temperature, salinity, rainfall, wind velocity, total bacteria, bacterial biomass, chlorophyll-a, phytoplankton, Anabaena sp., Merismopedia sp. and T. erythraeum densities were measured in surface water. Centric and pennate diatoms, Anabaena sp. and Merismopedia sp. were most abundant at 15 m isobath, while dinoflagellate abundance was relatively constant among stations. Similarl...

  14. A novel hepatitis B virus genotyping system by using restriction fragment length polymorphism patterns of S gene amplicons

    Institute of Scientific and Technical Information of China (English)

    Guo-Bing Zeng; Shu-Juan Wen; Zhan-Hui Wang; Li Yan; Jian Sun; Jin-Lin Hou

    2004-01-01

    AIM: Traditional hepatitis B virus (HBV) genotyping methods using restriction fragment length polymorphism (RFLP) can reliably identify genotypes A to F. As HBV genotypes G and H have been recently identified, this study was to establish an accurate and simple genotyping method for all eight HBV genotypes (A to H).METHODS: Two hundred and forty HBV small S sequences obtained from GeneBank were analysed for restriction enzyme sites that would be genotype-specific. Restriction patterns following digestion with restriction enzymes BsrⅠ,StyⅠ, DpnⅠ, HpaⅡ, and EaeⅠ, were determined to identify all eight HBV genotypes. Mixed genotype infections were confirmed by cloning and further RFLP analysis.RESULTS: The new genotyping method could identify HBV genotypes A to H. Genotypes B and C could be determined by a single step digestion with BsrⅠ and StyⅠ in parallel. This was particularly useful in the Far East where genotypes B and C are predominant. Serum samples from 187 Chinese HBV carriers were analysed with this genotyping system, and the genotype distribution was 1.1% (2), 51.9% (97), 40.6% (76) and 4.8%(9) for genotypes A, B, C, and D, respectively. Mixed genotypes were found in only 3 patients (1.6%). Sequence data analysis confirmed the validity of this new method.CONCLUSION: This HBV genotyping system can identify all eight HBV genotypes. It is accurate and simple, and can be widely used for studies on HBV genotyping.

  15. Differences in ovarian aging patterns between races are associated with ovarian genotypes and sub-genotypes of the FMR1 gene

    Directory of Open Access Journals (Sweden)

    Gleicher Norbert

    2012-09-01

    Full Text Available Abstract Background Ovarian aging patterns differ between races, and appear to affect fertility treatment outcomes. What causes these differences is, however, unknown. Variations in ovarian aging patterns have recently been associated with specific ovarian genotypes and sub-genotypes of the FMR1 gene. We, therefore, attempted to determine differences in how functional ovarian reserve (FOR changes with advancing age between races, and whether changes are associated with differences in distribution of ovarian genotypes and sub-genotypes of the FMR1 gene. Methods We determined in association with in vitro fertilization (IVF FOR in 62 young Caucasian, African and Asian oocyte donors and 536 older infertility patients of all three races, based on follicle stimulating hormone (FSH, anti-Müllerian hormone (AMH and oocyte yields, and investigated whether differences between races are associated with differences in distribution of FMR1 genotypes and sub-genotypes. Results Changes in distribution of mean FSH, AMH and oocyte yields between young donors and older infertility patients were significant (all P FMR1 genotypes and sub-genotypes in patients varied significantly between races, with Asians demonstrating fewer het-norm/low sub-genotypes than Caucasians and Africans (P = 0.012. Conclusion FOR changes in different races at different rates, and appears to parallel ovarian FMR1 genotypes and sub-genotype distributions. Differences in ovarian aging between races may, therefore, be FMR1-associated.

  16. Axiom turkey genotyping array

    Science.gov (United States)

    The Axiom®Turkey Genotyping Array interrogates 643,845 probesets on the array, covering 643,845 SNPs. The array development was led by Dr. Julie Long of the USDA-ARS Beltsville Agricultural Research Center under a public-private partnership with Hendrix Genetics, Aviagen, and Affymetrix. The Turk...

  17. Violence in Schools in Brazil.

    Science.gov (United States)

    Pino, Angel

    1995-01-01

    The causes of violence in schools, as in society, are multiple and complex; they are rooted in the intolerable economic and social conditions created by Brazil's development model, characterized by unequal wealth distribution, widespread poverty, and an exclusive society. By mirroring this exclusionary process, the educational system is inherently…

  18. Origin and Evolution of Dengue Virus Type 3 in Brazil

    OpenAIRE

    Josélio Maria Galvão de Araújo; Gonzalo Bello; Hector Romero; Rita Maria Ribeiro Nogueira

    2012-01-01

    The incidence of dengue fever and dengue hemorrhagic fever in Brazil experienced a significant increase since the emergence of dengue virus type-3 (DENV-3) at the early 2000s. Despite the major public health concerns, there have been very few studies of the molecular epidemiology and time-scale of this DENV lineage in Brazil. In this study, we investigated the origin and dispersion dynamics of DENV-3 genotype III in Brazil by examining a large number (n = 107) of E gene sequences sampled betw...

  19. Radiopharmacy education in Brazil

    Directory of Open Access Journals (Sweden)

    Ralph Santos-Oliveira

    2014-01-01

    Full Text Available The number of schools of pharmacy has been increasing each year in Brazil. From 2002 to 2013 over 300 new schools were opened in Brazil with a final number of 415 schools of pharmacy in operation around the country. Of these schools, only 28 schools offer a course in radiopharmacy (7.77%. However, the demand for such trained professionals has grown exponentially in Brazil, especially following amendment 49 (February 2006 that broke the monopoly on the production, distribution, and marketing of short half-life radiopharmaceuticals, and the recent constitutional amendment project 517/2010, which was approved in the last instance and is waiting for final approval by the President. Thus, in this scenario, there are a total of 417 radiopharmacy services across the country waiting for qualified professionals to fill posts. However, while there are insufficient trained professionals, radiopharmacy services under the aegis of Agencia Nacional de Vigilancia Sanitaria - Brazilian Health Surveillance Agency allow biomedical scientists and biologists to perform specialized functions as developed in radiopharmacy services without the presence of radiopharmacists.

  20. Comparison of methods for analysis of selective genotyping survival data

    Directory of Open Access Journals (Sweden)

    Dekkers Jack CM

    2006-11-01

    Full Text Available Abstract Survival traits and selective genotyping datasets are typically not normally distributed, thus common models used to identify QTL may not be statistically appropriate for their analysis. The objective of the present study was to compare models for identification of QTL associated with survival traits, in particular when combined with selective genotyping. Data were simulated to model the survival distribution of a population of chickens challenged with Marek disease virus. Cox proportional hazards (CPH, linear regression (LR, and Weibull models were compared for their appropriateness to analyze the data, ability to identify associations of marker alleles with survival, and estimation of effects when all individuals were genotyped (full genotyping and when selective genotyping was used. Little difference in power was found between the CPH and the LR model for low censoring cases for both full and selective genotyping. The simulated data were not transformed to follow a Weibull distribution and, as a result, the Weibull model generally resulted in less power than the other two models and overestimated effects. Effect estimates from LR and CPH were unbiased when all individuals were genotyped, but overestimated when selective genotyping was used. Thus, LR is preferred for analyzing survival data when the amount of censoring is low because of ease of implementation and interpretation. Including phenotypic data of non-genotyped individuals in selective genotyping analysis increased power, but resulted in LR having an inflated false positive rate, and therefore the CPH model is preferred for this scenario, although transformation of the data may also make the Weibull model appropriate for this case. The results from the research presented herein are directly applicable to interval mapping analyses.

  1. Distribution of phlebotomine fauna (Diptera: Psychodidae) across an urban-rural gradient in an area of endemic visceral leishmaniasis in northern Brazil.

    Science.gov (United States)

    Oliveira, Davi Marcos Souza de; Saraiva, Elvira Maria; Ishikawa, Edna Aoba Yassui; Sousa, Adelson Alcimar Almeida de; Silva, Edilene Oliveira da; Silva, Ivoneide Maria da

    2011-12-01

    The number of visceral leishmaniasis (VL) cases has increased over the past 10 years in Brazil, especially in the North and Northeast regions of the country. The aim of this study was to evaluate the urbanisation of VL vectors in Barcarena, Pará, an area in northern Brazil where VL is endemic. Sandflies were captured using Centers for Disease Control (CDC) light traps along an urban-rural gradient. The CDC traps were installed inside hen houses at a height of 150 cm. A total of 5,089 sandflies were collected and 11 species were identified. The predominant species was Lutzomyia longipalpis (rate of 95.15%), which suggests its participation in the transmission of VL. A total of 1,451 Lu. longipalpis females were dissected and no Leishmania infections were detected. Most of the sandflies were captured at the border of a forest (88.25%) and no flies were captured in the urban area, which suggests that transmission is still restricted to rural sites. However, the fact that a specimen was collected in an intermediate area indicates that urbanisation is a real possibility and that vector monitoring is important.

  2. Use of space and temporal distribution of Trichechus manatus manatus Linnaeus in the region of Sagi, Rio Grande do Norte State, Brazil (Sirenia, Trichechidae

    Directory of Open Access Journals (Sweden)

    Danielle Paludo

    2002-03-01

    Full Text Available The marine manatee Trichechus manatus manatus Linnaeus, 1758 is endangered in the Brazilian Atlantic coast due to indiscriminate hunting practiced in the past, and to the low reproductive rate of the species. This work studies its use of space and temporal occurrence in the coastal area of Sagi, Northeastern Brazil. Daily observations from the beach of the animals in the sea were made during daytime from 1990 through 1993. The presence of reefs covered with marine algae is a determinant factor in the occurrence of the manatee. They were sighted feeding on algae that grow over the reefs close to beaches that were of high energy during high tide. The range used by manatees shifted according to tide. They occur in depths of 0.4 to 3.8 m; the distance from the beach varies according to the tide level. They show marked seasonality of occurrence, with higher frequencies in December - January and lowest in June - July. Two possible causes of the seasonal occurrence are discussed. Sagi is important for the conservation of the species in Northeastern Brazil as feeding and reproductive grounds.

  3. Distribution of phlebotomine fauna (Diptera: Psychodidae across an urban-rural gradient in an area of endemic visceral leishmaniasis in northern Brazil

    Directory of Open Access Journals (Sweden)

    Davi Marcos Souza de Oliveira

    2011-12-01

    Full Text Available The number of visceral leishmaniasis (VL cases has increased over the past 10 years in Brazil, especially in the North and Northeast regions of the country. The aim of this study was to evaluate the urbanisation of VL vectors in Barcarena, Pará, an area in northern Brazil where VL is endemic. Sandflies were captured using Centers for Disease Control (CDC light traps along an urban-rural gradient. The CDC traps were installed inside hen houses at a height of 150 cm. A total of 5,089 sandflies were collected and 11 species were identified. The predominant species was Lutzomyia longipalpis (rate of 95.15%, which suggests its participation in the transmission of VL. A total of 1,451 Lu. longipalpis females were dissected and no Leishmania infections were detected. Most of the sandflies were captured at the border of a forest (88.25% and no flies were captured in the urban area, which suggests that transmission is still restricted to rural sites. However, the fact that a specimen was collected in an intermediate area indicates that urbanisation is a real possibility and that vector monitoring is important.

  4. Distribution of alpha and beta thalassaemia genotypes in childbearing age people in Shanwei%汕尾市育龄人群中α、β地中海贫血基因型的分布调查

    Institute of Scientific and Technical Information of China (English)

    孙妙婷; 董响波; 黄琦玲

    2014-01-01

    objective To understand the distribution of alpha and beta thalassaemia genotypes in childbearing age people in Shanwei.Methods Pregnant woment and their spouses 1200 cases were chosen from the hospitals of each area from January 1st to December 1st 2013 as study objects using cluster sampling method.Alpha beta thalassaemia were investigated respectively.Results Among the 1200 blood samples,6 kinds of alpha thalassaemia genotypes were detected,with a total of 62 cases,63 alleles.The alpha thalassaemia gene carrying rate was 5.25% (63/1200).The genotype composition:31-a3.7/aa,49.21% of the total; 18-aSEA/aa,28.57% of the total; 7-a4.2/aa,11.11% of the total; 2-aTHAI/aa,3.17% of the total; 3--SEA/--SEA,4.76% of the total; 2-aCSa/aa,3.17% of the total.13 cases were detected with beta thalassemia gene,with a beta thalassemia gene carrying rate of 1.08%.There were five genetic types of beta thalassemia gene including IVS-2-654 (C → T) and-28 (A → G) (both accounted for 23.08%),CD41/42 (-TCTT) (30.77%),CD17 (A → T) (15.38%),and beta ECD26 (C → A) (7.69%).Conclusions The alpha thalassaemia gene carrying rate and beta thalassaemia carrying rate of Shanwei are relatively lower among the whole province.However,but the analysis of the data showed that the carrying rate was high,thalassaemia genes were carried mainly by people,most of which are natives,in regions with complex genetic background.%目的 了解汕尾市育龄人群中α、β地中海贫血基因型的分布情况.方法 采用整群抽样方法选择2013年1月1日至2013年12月31日各地区部分住院分娩和引产的孕产妇、配偶共计1200例作为研究对象,分别进行α-地中海贫血和β-地中海贫血调查.结果 在1200份血液标本中共计检测出6种α-地中海贫血基因型,共计62例,63个等位基因,α-地中海贫血基因携带率为5.25%(63/1200).其基因型构成比:-a3.7/aa 31个,占总数的49.21%;-aSEA/aa 18个,占总数的28.57

  5. 陕西省宫颈人乳头瘤病毒感染亚型分布特征分析%Distribution characteristics of human papillomavirus infection genotypes in cervix of women in Shaanxi Province

    Institute of Scientific and Technical Information of China (English)

    樊静; 李娜; 张忠明; 安瑞芳; 李娇; 赵静妮; 邹余粮; 苟文丽; 杨筱凤

    2015-01-01

    目的:明确陕西省及周边地区成年女性宫颈人乳头瘤病毒( HPV)感染基因亚型的分布及年龄特征。方法分析2009年11月至2015年4月在西安交通大学第一附属医院妇科门诊22306例宫颈样本21种HPV基因分型检测结果。 HPV分型检测采用凯普医用核酸分子快速导流杂交基因芯片技术。结果 HPV总感染率为28.84%。高危型HPV(16、18、31、33、35、39、45、51、52、53、56、58、59、66、68)感染率为26.26%,占HPV阳性标本的91.05%;低危型HPV(6、11、42、43、44、81)感染率为4.81%;高危/低危HPV混合感染率为2.22%。感染率最高的基因亚型为HPV16(9.31%),其他常见高危亚型依次为HPV52(5.08%)、HPV58(4.99%)、HPV18(2.09%)、HPV31(1.95%)、HPV39(1.86%)、HPV33(1.74%);常见低危亚型为 HPV6(2.02%)、11(1.56%)、81(1.02%)。 HPV总体感染率和高危HPV亚型感染高峰年龄在30~34岁之间,以后随年龄增长逐渐降低,各年龄组间HPV检出率有显著性差异(χ2值为4.743~85.971,均P<0.05)。结论陕西省及周边地区女性宫颈HPV处于较高的感染水平;其HPV感染基因型符合亚洲人群分布规律,但有一定区域性差异;随着年龄的增高,高危型HPV感染比例逐步缓慢增加。该结果对区域宫颈癌防治和疫苗制备具指导意义。%Objective To analyze HPV genotype distribution and age characteristics among women in Shaanxi Province and areas nearby. Methods A total of 22 306 cervical scraping samples were collected from the outpatients for cervical cancer screening in the First Affiliated Hospital of Xi’ an Jiaotong University during November 2009 to April 2015.Twenty-one HPV genotypes were examined by PCR-based hybridization gene chip assay.Results The overall HPV infection rate was 28.84%.The infection rate of high-risk HPV (16, 18, 31

  6. Phosphorus Use Efficiency by Brazilian Upland Rice Genotypes Evaluated by the 32P Dilution Technique

    International Nuclear Information System (INIS)

    The objectives of this work were to identify the most efficient upland rice genotypes in phosphorus (P) utilization, and to verify if P from the seed affects the classification of upland rice genotypes on P uptake efficiency. The experiment was conducted in a greenhouse of the Center for Nuclear Energy in Agriculture (CENA/USP), Piracicaba, Sao Paulo, Brazil, using the 32P isotope technique, and plants were grown in pots with samples of dystrophic Typic Haplustox (Oxisol). The experimental design was completely randomized with four replications. The treatments consisted of 47 upland rice genotypes and two standard plant species, efficient or inefficient in P uptake. The results were assessed through correlation and cluster analysis (multivariate). The Carisma upland rice genotype was the most efficient in P uptake, and Caripuna was the most efficient on P utilization. The P derived from seed does not influence the identification of upland rice genotypes in P uptake efficiency. (author)

  7. Complete Genome Sequences of Two Dengue Virus Serotype 1 Genotype V Strains from Different Lineages

    Science.gov (United States)

    Vedovello, Danila; Menegaldo, Tauyne; Biselli-Périco, Joice M.; Ullmann, Leila Sabrina; Araújo Junior, João Pessoa

    2016-01-01

    Previous phylogenetic studies involving dengue virus serotype 1 (DENV1) have shown several lineages of genotype V circulating worldwide. After sequencing the complete genome of strains from São José do Rio Preto, São Paulo, Brazil, we identified a list of 50 different amino acids that differ between the two lineages, announced here. PMID:27688321

  8. Complete Genome Sequences of Two Dengue Virus Serotype 1 Genotype V Strains from Different Lineages.

    Science.gov (United States)

    Vedovello, Danila; Menegaldo, Tauyne; Biselli-Périco, Joice M; Ullmann, Leila Sabrina; Araújo Junior, João Pessoa; Nogueira, Maurício Lacerda

    2016-01-01

    Previous phylogenetic studies involving dengue virus serotype 1 (DENV1) have shown several lineages of genotype V circulating worldwide. After sequencing the complete genome of strains from São José do Rio Preto, São Paulo, Brazil, we identified a list of 50 different amino acids that differ between the two lineages, announced here. PMID:27688321

  9. The Impact of Geomorphology and Human Disturbances on the Faunal Distributions in Tiquara and Angico Caves of Campo Formoso, Bahia, Brazil

    Directory of Open Access Journals (Sweden)

    André Vieira de Araujo

    2015-03-01

    Full Text Available Characterization of fauna is important for the understanding of communities and ecosystems, enabling the design of actions for conservation. In the present piece of work, we identified total 45 morphospecies belonging to the order Acarina, Pseudoscorpionida, Dictyoptera, Araneida, Amblypygi, Isopoda, Plecoptera, Amphipoda, Zygentoma, Spirostreptida, Coleoptera, Collembola, Diptera, Ensifera, Heteroptera, Hymenoptera, Lepidoptera and Psocoptera from two distinct types of caves of Campo Formoso, state of Bahia in Brazil. It was made to provide subsidies for conservation studies. The targeted caves were Tiquara Cave suffered for many years from saltpeter extraction and Angico Cave less visited cave having high tourist potential. Though the conservation status is much better in Angico cave, but we found comparatively more morphospecies in Tiquara cave.

  10. Predictors of hepatitis B virus genotype and viraemia in HIV-infected patients with chronic hepatitis B in Europe

    DEFF Research Database (Denmark)

    Soriano, Vincent; Mocroft, Amanda; Peters, Lars;

    2010-01-01

    Both natural history and treatment outcome of hepatitis B virus (HBV) infection are influenced by genotypes and viral load. Information about factors determining HBV genotype distribution and viraemia in HIV/HBV-co-infected patients is scarce.......Both natural history and treatment outcome of hepatitis B virus (HBV) infection are influenced by genotypes and viral load. Information about factors determining HBV genotype distribution and viraemia in HIV/HBV-co-infected patients is scarce....

  11. Human papillomavirus genotypes in asymptomatic young women from public schools in Rio de Janeiro, Brazil Genótipos de papilomavírus humanos em mulheres jovens assintomáticas de escolas públicas do Rio de Janeiro, Brasil

    Directory of Open Access Journals (Sweden)

    Ledy Horto Santos Oliveira

    2010-02-01

    Full Text Available INTRODUCTION: The aim of this work was to survey HPV information from a random population of young women from Rio de Janeiro, Brazil. METHODS: This cross-sectional study included cervical samples from 241 female students. To determine human papillomavirus status, polymerase chain reaction amplification was performed. HPV typing was determined by restriction fragment length polymorphism analysis. Demographic data, life style, sexual and gynecological history were obtained through use of a structured questionnaire. RESULTS: The average age of the women was 19.6 years-old (SD=3.4 years. HPV prevalence was 27.4%. Nineteen different HPV genotypes were detected, including 13 high risk types. HPV 16 was the most prevalent type (6.2%, followed by 31 (4.1 % and 66 (3.7%. Most of the oncogenic types belonged to the A9 species (28/48. The frequency of women infected by at least one oncogenic type was significantly higher than those only infected by low risk types (18.7% versus 7.5%. Cervical changes were detected in 12.5% of the sample and were significantly linked to infection with HPV types of the A9 species. Demographic variables, sexual initiation, or number of sexual partners were not associated with HPV prevalence, variety of HPV genotypes or oncogenic types. CONCLUSIONS: The relative frequency of HPV genotypes other than vaccine types in young females should be taken into account when evaluating vaccination strategies. Due to the high prevalence of HPV infection among the population studied, implementation of sex education in schools, promotion of condom use and an organized screening program to prevent cervical cancer must be encouraged for this age group.INTRODUÇÃO: O objetivo deste trabalho foi investigar a infecção por HPV em uma população randômica de mulheres jovens no estado do Rio de Janeiro, Brasil. MÉTODOS: Este estudo incluiu amostras cervicais de 241 jovens. A detecção de papilomavírus humanos foi realizada pela reação da

  12. Analyzing natural gas distribution in Brazil through EVA (Economic Value Added); Analise economico-financeira da industria de distribuicao de gas natural brasileiro sob a otica da geracao de valor

    Energy Technology Data Exchange (ETDEWEB)

    Marques, Fernando Rodrigues; Parente, Virginia [Universidade de Sao Paulo (USP), SP (Brazil). Inst. de Eletrotecnica e Energia

    2008-07-01

    Although the Brazilian gas market has been showing steady growth and positive news regarding new discoveries, it is not immune to be questioned about its capacity of value generation, and, in particular, if the new companies, operating in distribution have generated it. Value creation, and its management, has become one of the main activities of the modern corporation in order to align the management and shareholders interests. Despite its advancements, the Brazilian NG industry can be considered at its early stages when compared to those of other countries whose industry is more traditional and mature. The process of unbundling of the gas industry, as well as of the public utilities, has become one of the icons of the Brazilian government policies since the mid 90's. Taking into account regulatory change which had only allowed in the last couple of decades the participation of private ownership into the domestic gas market, a follow up of these companies' performances is of interest. Following the concept of value generation, the present study examines the Brazilian gas distribution sector. Based on a detailed analysis of the financial statements of some of the main companies in the sector, that together represent around 68% of the total Brazilian market, this paper verifies whether the gas distribution sector have presented a positive or negative EVA{sup R}, in other words, if, during the period from 2002 to 2007, these newly companies in the gas industry have created or destroyed value in Brazil. (author)

  13. The obstacles for the investments in natural gas distribution infrastructure in Brazil; Os obstaculos aos investimentos na rede de distribuicao de gas natural no Brasil

    Energy Technology Data Exchange (ETDEWEB)

    Almeida, Edmar Luiz Fagundes de; Bueno, Salua Saud; Selles, Vitor [Universidade Federal do Rio de Janeiro (UFRJ), RJ (Brazil). Inst. de Economia

    2004-07-01

    This paper analyses the main obstacles for the expansion of the Brazilian gas distribution pipelines infrastructure. The paper examines the evolution of investments in the gas chain and highlights the existence of an important unbalance between the level of investments in the upstream and transportation segments and the level of investment in the distribution network. It is clear that the level of investments in the distribution segment is not following the pace of expansion of the other segments. Given this conclusion, the paper examines the potential for increasing the level of investment in the distribution segment by augmenting the debt level of distribution companies. By analyzing the main distribution companies' financial statements, the paper shows that they there is room for an expansion in investments through financial leverage. Finally, the paper examines the main financing obstacles that impede the companies to increase their investment level. (author)

  14. Land reform and landholdings in Brazil

    OpenAIRE

    Assunção, Juliano

    2006-01-01

    Land and wealth are closely related in rural Brazil, a country characterized by high levels of inequality in terms of income or landholdings. After presenting a historical retrospective of land concentration and land reform in Brazil, this study evaluates the impact of the land reform programme undertaken in the 1990s on land ownership and land distribution. It is shown that the programme increased landownership among poor rural families and those with less educated household heads, reducing ...

  15. Concentrations and distributions of metals in tissues of stranded green sea turtles (Chelonia mydas) from the southern Atlantic coast of Brazil

    Energy Technology Data Exchange (ETDEWEB)

    da Silva, Cinthia Carneiro [Programa de Pós-Graduação em Ciências Fisiológicas – Fisiologia Animal Comparada, Instituto de Ciências Biológicas, Universidade Federal do Rio Grande, Av. Itália km 8, 96203-900, Rio Grande, Rio Grande do Sul (Brazil); Varela, Antonio Sergio; Barcarolli, Indianara Fernanda [Instituto de Ciências Biológicas, Universidade Federal do Rio Grande, Av. Itália km 8, 96203-900, Rio Grande, Rio Grande do Sul (Brazil); Bianchini, Adalto, E-mail: adaltobianchini@furg.br [Programa de Pós-Graduação em Ciências Fisiológicas – Fisiologia Animal Comparada, Instituto de Ciências Biológicas, Universidade Federal do Rio Grande, Av. Itália km 8, 96203-900, Rio Grande, Rio Grande do Sul (Brazil); Instituto de Ciências Biológicas, Universidade Federal do Rio Grande, Av. Itália km 8, 96203-900, Rio Grande, Rio Grande do Sul (Brazil)

    2014-01-01

    Silver (Ag), cadmium (Cd), copper (Cu), lead (Pb) and zinc (Zn) concentrations were analyzed in tissues of juvenile green sea turtles (Chelonia mydas) found stranded along the southern Atlantic coast in Brazil. Green sea turtles were collected (n = 29), measured (curved carapace length: CCL) and had their muscle, liver, and kidney dissected for metal concentration measurements. Sex was identified in 18 individuals (10 females and 8 males) through gonad histology. No gender differences in CCL and tissue metal concentrations were observed. In the muscle, there was a negative correlation between CCL and Cd and Cu concentrations. Metal concentrations were lower in the muscle than in the liver and kidney. Zn concentration in the muscle was the highest of all metals analyzed (16.6 mg/kg). The kidney showed the highest concentrations of Pb, Cd and Zn (5.4, 28.3 and 54.3 mg/kg, respectively), while the liver had the highest values of Ag and Cu (0.8 and 100.9 mg/kg, respectively). Tissue Ag, Zn and Cd concentrations were similar to those found in green sea turtles from other regions while Cu and Pb values were elevated, likely due to the metal-rich water and sediment reported in the collection area. In the liver and kidney, concentrations of non-essential (Ag, Cd and Pb) and essential (Cu or Zn) metals were positively correlated, likely due to an induced metallothionein synthesis to protect tissue against the toxic effect of metals. This is the first study to report and correlate the concentrations of essential and non-essential metals in tissues of green sea turtles in the Brazilian southern Atlantic coast, an important feeding and developing area for this turtle species. - Highlights: •Juvenile female and male green sea turtles have similar concentrations of metals. •Kidney accumulated more Cd, Pb and Zn while liver accumulated more Ag and Cu. •Cu and Pb concentrations are elevated in liver of sea turtles from southern Brazil. •Concentrations of Cd and Cu in

  16. Infection and genotype distribution of human papilloma virus (HPV) in female in Wenzhou%温州地区女性人乳头瘤病毒的感染情况及亚型分布特征分析

    Institute of Scientific and Technical Information of China (English)

    屠鸿翔; 虞丹丹; 陈占国; 周武; 陈栎江

    2012-01-01

    目的:了解本地区女性HPV的感染情况及型别分布,以助于HPV疫苗的研制和应用.方法:采用快速分子导流杂交分型技术对4314例女性HPV的感染情况及型别分布进行分析.结果:HPV感染阳性率为41.68% (1798/4314).10组年龄组中筛查人群以31岁~50岁的妇女为主,占72.02% (3107/4314);HPV阳性率以21岁~25岁(56.86%)及≥60岁(64.39%)的年龄组较高.各年龄组均以单重感染为主,其次为双重感染.在单重感染下,高危亚型均以HPV16、52、58为主,其次为HPV18、33;低危亚型以HPV6、11为主;常见亚型以HPV53、cp8304(81)为主.结论:温州地区女性HPV感染以单重感染为主,高危亚型优势型别为HPV16、52、58,低危亚型优势型别为HPV6、11,常见亚型优势型别为HPV53、cp8304(81).%Objective: To investigate the infection and genotype distribution of human papilloma virus in female in Wenzhou for development and application of HPV vaccine. Methods; The cervical samples of 4314 women were detected with flow -through hybridization and gene chip on HPV subtype was also analyzed. Results; The HPV positive rate was 41. 68% (1798/4314). Screened women were mainly between 31 to 50 years old in 10 age groups, accounting for72.02%. The higher HPV positive rates were occupied by 21 to 25 years old group(56.86% ) and =60 years old group(64. 39%). The common HPV infection in 10 age groups was mainly single infection, secondly double infection. In single HPV infection, HPV16, HPV52 and HPV58 were the most frequent high risk subtypes, HPV18 and HPV33 were the secondly frequent high risk subtypes; HPV6 and HPV11 were the most frequent low risk subtypes;HPV53 and HPV cp8304(81) were the most frequent common subtypes. Conclusion; The most common HPV infection was single infection in Wenzhou. The predominate high risk subtype was HPV16, HPV52 and HPV58, the predominate low risk subtype was HPV6 and HPV11, the predominate common subtype was HPV53 and HPVcp

  17. Analysis of HPV infection in cervix and genotype distribution of women in Wuhan city%武汉地区女性宫颈人乳头状瘤病毒感染与亚型分布研究

    Institute of Scientific and Technical Information of China (English)

    肖晗; 孙红; 向飞艳; 夏倩; 刘秀珍; 向贇

    2016-01-01

    OBJECTIVE To investigate HPV prevalence in cervix and genotype distribution of women in Wuhan city and enrich the molecular epidemiology data of HPV infection ,so as to provide reference for prevention against HPV infection and cervical cancer .METHODS Samples of cervical exfoliated cells were collected from 9083 women patients in Wuhan Medical and Health Center for Women and Children from May 2014 to Jun .2015 .Twenty‐one HPV subtypes were detected by gene amplification and flow‐through hybridization technology .RESULTS The total infection rate of HPV was 17 .46% (1586/9083) .Among 1586 positive samples ,the single‐subtype infection ac‐counted for 75 .35% and the multiple‐subtype infection 24 .65% .HPV‐52(4 .18% ) ,HPV‐16 (2 .49% ) ,HPV‐58 (2 .33% ) ,HPV‐39(1 .82% ) and HPV‐51(1 .63% ) were the top five prevalent subtypes .The highest HPV posi‐tive rate 38 .46% was found in below 20 years age group (P< 0 .05) .In addition ,the positive rates of 21‐25 years ,45‐50 years and 51‐55 years age group were all above 20% .There was no significant statistical difference on the HPV infection rate among different months .CONCLUSION The HPV infection rate in women in Wuhan re‐gion is relatively higher ,and the multiple‐subtype infection accounts for a considerable proportion . HPV‐52 , HPV‐16 and HPV‐58 were the top three prevalent subtypes .%目的:研究武汉地区女性宫颈人乳头状瘤病毒(HPV )感染状况及基因亚型分布,丰富 HPV感染的分子流行病学资料,为制定防治HPV感染及宫颈癌的措施提供依据。方法收集2014年5月-2015年6月在武汉市妇女儿童医疗保健中心就诊和体检的女性患者宫颈刷取物标本9083例,采用基因扩增及导流杂交技术进行21种 H PV亚型的基因分型检测。结果共检出 H PV阳性标本1586例,感染率为17.46%,其中,单一亚型感染占75.35%,多重感染占24.65%;检出频率最高的前5

  18. 常州地区女性人乳头瘤病毒感染及基因型分析%Analysis on the Human Papillomavirus (HPV) Genotypes Distribution Pattern in Infected Women in Changzhou Area

    Institute of Scientific and Technical Information of China (English)

    戚传平; 许联红; 蒋立新; 王永仿

    2011-01-01

    Objective To s:udy the distribution pattern of HPV genotypes in infected women in Changzhou area,so as to provide the evidences for the prevention of cervical diseases and the clinical application of HPV vaccines. Methods A total of 1 016 women with sexy life were detected for 21 HPV subtypes using flow-through hybridization.Results 20 different subtypes were found in 1 016 women. HPV DNA was detected in 27. 8% of all the women. The high-risk HPV was detected in 25- 6% ,the low-risk HPV was detected in 3. 4% and 6. 2% patients were infected with multiple-type HPV. The most commonly found high-risk types were HPV16tHPV58,HPV52,HPV31 and HPV68. The HPV infection rate increased a-long with the upgrade of the cervical lesions. Conclusion The subtype infection rate in Changzhou area ranked as high-risk HPV16,58,52,31 and 68. So,early screening and controlling HPV infection could decrease effectively the morbidity of cervical cancer.%目的 了解江苏常州地区妇女宫颈人乳头瘤病毒(HPV)感染的分布情况,为该地区宫颈疾病防治及疫苗的投入使用提供一定的依据.方法 采用导流杂交基因芯片技术对1 016例有性生活史妇女宫颈脱落细胞进行21种HPV基因型检测.结果 在被调查的1 016例妇女中共有20种HPV亚型被检测出来.HPV感染总阳性率为27.8%,高危型检出率为25.6%,低危型为3.4%,混合型感染为6.2%.HPV感染阳性排在前5位的亚型从高到低依次为HPV16,58,52,31和68.随着宫颈疾病病变级别升高,HPV感染率呈正向增加趋势.结论 常州地区HPV亚型感染分布以高危型HPV16,58,52,31,68为主,早期筛查和控制HPV感染能有效降低宫颈癌的发病率.

  19. 新疆维族妇女感染的HPV型别分布的研究%Distribution and Prevalence of Human Papillomavirus(HPV)Genotypes in 2473 Cytological Samples OfXinjiang Uyghur Women

    Institute of Scientific and Technical Information of China (English)

    郑义; 杨磊; 高丽敏; 井明霞; 张金丽; 芮东升; 王英红; 阿曼古丽; 何玲; 赵娟

    2012-01-01

    Objective:To investigate the types distribution of human papillomavirus(HPV)in women in Xinjiang Uyghur women.Methods:Women between 30 and 59 were chosen in cluster stratified sampling from XiaPuTaoLe Town,Xinjiang and received a population-based cervical cancer screening After consent every woman was detected and genotyped HPV DNA by PCR and Gene Chip.Results:2473 women were chosen.The total infection rate of HPV was 9.1%.The positive rate of HPV-16 infection was 6.6% among the High-Risk HPV indicating it was the most common infection.Other High-Risk HPV infection rates were ranked from high to low:HPV-59,HPV-56,HPV-18,HPV-33,HPV-58,HPV-51,HPV-31,HPV-45,HPV-52,HPV-35,HPV-39,HPV-68.HPV-11 was the most common infection among the Low-Risk HPV types.Other Low-Risk HPV infection rates were ranked from high to low:HPV-42,HPV-43,HPV-6,HPV-53,HPV-66,HPV-73.HPV-44,-83,-MM4 were not detected.The multiple infection rate was 34.2%.Conclusion:HPV-16 was the most common types in the study population.The next most common types of HPV were HPV59,56,18,33.Xinjiang Uighur women had the relatively higher risk of suffering HPV59 infection indicating the specificity of HPV infection in Xinjiang Uighur women.%目的:探讨人乳头瘤状病毒(Human papillomavirus,HPV)在新疆南部维族妇女人群中的型别分布情况.方法:以年龄在30-59岁的新疆伽师县夏普吐勒乡维吾尔族妇女人群为基础进行筛查,签署知情同意书后,采集受试者宫颈脱落细胞,利用PCR和基因芯片技术检测HPV DNA并分型.结果:共2473名妇女入选.HPV总的感染率为9.1%,高危型中HPV-16的感染率最高为6.9%,其他高危型的感染率从高到低依次为:HPV-59、HPV-56、HPV-18、HPV-33、HPV-58、HPV-51、HPV-31、HPV-45、HPV-52、HPV-68、HPV-35、HPV-39.低危型中HPV11感染率最高,其他低危型的感染率从高到低依次为HPV-42、HPV-43、HPV-6、HPV-53、HPV-66、HPV-73.HPV-44、-83、-MM4没检测到.多重感染率为34.2

  20. Origin and evolution of dengue virus type 3 in Brazil.

    Directory of Open Access Journals (Sweden)

    Josélio Maria Galvão de Araújo

    Full Text Available The incidence of dengue fever and dengue hemorrhagic fever in Brazil experienced a significant increase since the emergence of dengue virus type-3 (DENV-3 at the early 2000s. Despite the major public health concerns, there have been very few studies of the molecular epidemiology and time-scale of this DENV lineage in Brazil. In this study, we investigated the origin and dispersion dynamics of DENV-3 genotype III in Brazil by examining a large number (n=107 of E gene sequences sampled between 2001 and 2009 from diverse Brazilian regions. These Brazilian sequences were combined with 457 DENV-3 genotype III E gene sequences from 29 countries around the world. Our phylogenetic analysis reveals that there have been at least four introductions of the DENV-3 genotype III in Brazil, as signified by the presence of four phylogenetically distinct lineages. Three lineages (BR-I, BR-II, and BR-III were probably imported from the Lesser Antilles (Caribbean, while the fourth one (BR-IV was probably introduced from Colombia or Venezuela. While lineages BR-I and BR-II succeeded in getting established and disseminated in Brazil and other countries from the Southern Cone, lineages BR-III and BR-IV were only detected in one single individual each from the North region. The phylogeographic analysis indicates that DENV-3 lineages BR-I and BR-II were most likely introduced into Brazil through the Southeast and North regions around 1999 (95% HPD: 1998-2000 and 2001 (95% HPD: 2000-2002, respectively. These findings show that importation of DENV-3 lineages from the Caribbean islands into Brazil seems to be relatively frequent. Our study further suggests that the North and Southeast Brazilian regions were the most important hubs of introduction and spread of DENV-3 lineages and deserve an intense epidemiological surveillance.

  1. Origin and evolution of dengue virus type 3 in Brazil.

    Science.gov (United States)

    de Araújo, Josélio Maria Galvão; Bello, Gonzalo; Romero, Hector; Nogueira, Rita Maria Ribeiro

    2012-01-01

    The incidence of dengue fever and dengue hemorrhagic fever in Brazil experienced a significant increase since the emergence of dengue virus type-3 (DENV-3) at the early 2000s. Despite the major public health concerns, there have been very few studies of the molecular epidemiology and time-scale of this DENV lineage in Brazil. In this study, we investigated the origin and dispersion dynamics of DENV-3 genotype III in Brazil by examining a large number (n=107) of E gene sequences sampled between 2001 and 2009 from diverse Brazilian regions. These Brazilian sequences were combined with 457 DENV-3 genotype III E gene sequences from 29 countries around the world. Our phylogenetic analysis reveals that there have been at least four introductions of the DENV-3 genotype III in Brazil, as signified by the presence of four phylogenetically distinct lineages. Three lineages (BR-I, BR-II, and BR-III) were probably imported from the Lesser Antilles (Caribbean), while the fourth one (BR-IV) was probably introduced from Colombia or Venezuela. While lineages BR-I and BR-II succeeded in getting established and disseminated in Brazil and other countries from the Southern Cone, lineages BR-III and BR-IV were only detected in one single individual each from the North region. The phylogeographic analysis indicates that DENV-3 lineages BR-I and BR-II were most likely introduced into Brazil through the Southeast and North regions around 1999 (95% HPD: 1998-2000) and 2001 (95% HPD: 2000-2002), respectively. These findings show that importation of DENV-3 lineages from the Caribbean islands into Brazil seems to be relatively frequent. Our study further suggests that the North and Southeast Brazilian regions were the most important hubs of introduction and spread of DENV-3 lineages and deserve an intense epidemiological surveillance. PMID:22970331

  2. Recent transmission of drug-resistant Mycobacterium tuberculosis in a prison population in southern Brazil

    Science.gov (United States)

    Reis, Ana Julia; de David, Simone Maria Martini; Nunes, Luciana de Souza; Valim, Andreia Rosane de Moura; Possuelo, Lia Gonçalves

    2016-01-01

    ABSTRACT We conducted a cross-sectional, retrospective study, characterized by classical and molecular epidemiology, involving M. tuberculosis isolates from a regional prison in southern Brazil. Between January of 2011 and August of 2014, 379 prisoners underwent sputum smear microscopy and culture; 53 (13.9%) were diagnosed with active tuberculosis. Of those, 8 (22.9%) presented with isoniazid-resistant tuberculosis. Strain genotyping was carried out by 15-locus mycobacterial interspersed repetitive unit-variable-number tandem-repeat analysis; 68.6% of the patients were distributed into five clusters, and 87.5% of the resistant cases were in the same cluster. The frequency of drug-resistant tuberculosis cases and the rate of recent transmission were high. Our data suggest the need to implement an effective tuberculosis control program within the prison system.

  3. Effects of breed and nutrition on the productive traits of beef cattle in South East Brazil: Part 2-Tissue distribution and carcase composition.

    Science.gov (United States)

    Norman, G A; de Felicio, P E

    1982-01-01

    This paper is the second in a series recording the effects of breed and nutrition on the productive traits of Nelore, Guzerá, Charolais and Canchim cattle in south-east Brazil. Significant treatment variations were recorded in carcase bone weights but differences in meat to bone ratios were not sufficiently marked to demonstrate that bone might grow at the expense of muscle when animals are maintained on particularly severe nutritional regimes. A simple carcase weight to length ratio was shown to be highly correlated with total bone proportion but not with yield of saleable meat. Recovery of total saleable meat was only marginally and not significantly affected by breed but was significantly affected by treatment. The increased levels of fatness in the carcases of the zebu and, in particular, all animals finished in feedlot, that affected the yield of saleable meat, was also thought to be responsible for the decreased recovery of first quality meat in these carcases. In general, tissue differences were more noticeable between treatments than between breeds and reflected the varying nutritional status of the animals pre-slaughter. PMID:22054702

  4. Ecology of mosquitoes (Diptera: Culicidae in areas of Serra do Mar State Park, State of São Paulo, Brazil. II - Habitat distribution

    Directory of Open Access Journals (Sweden)

    Anthony Érico Guimarães

    2000-01-01

    Full Text Available The mosquito (Diptera: Culicidae ecology was studied in areas of Serra do Mar State Park, State of São Paulo, Brazil. Systematized biweekly human bait collections were made three times a day, for periods of 2 or 3 h each, in sylvatic and rural areas for 24 consecutive months (January 1991 to December 1992. A total of 24,943 adult mosquitoes belonging to 57 species were collected during 622 collective periods. Aedes scapularis, Coquillettidia chrysonotum, Cq. venezuelensis, Wyeomyia dyari, Wy. longirostris, Wy. theobaldi and Wy. palmata were more frequently collected at swampy and at flooded areas. Anopheles mediopunctatus, Culex nigripalpus, Ae. serratus, Ae. fulvus, Psorophora ferox, Ps. albipes and the Sabethini in general, were captured almost exclusively in forested areas. An. cruzii, An. oswaldoi and An. fluminensis were captured more frequently in a residence area. However, Cx. quinquefasciatus was the only one truly eusynanthropic. An. cruzii and Ae. scapularis were captured feeding on blood inside and around the residence, indicating that both species, malaria and arbovirus vectors respectively, may be involved in the transmission of these such diseases in rural areas.

  5. Phytoplankton and environmental factors in the Paraíba do Norte River Estuary, northeastern Brazil: composition, distribution and quantitative remarks

    Directory of Open Access Journals (Sweden)

    Roberto Sassi

    1991-01-01

    Full Text Available This paper reports the results obtained from studies on the estuarine phytoplankton of the Paraíba do Norte River in northeastern Brazil. Surface and near-bottom samples were taken at four stations numbered seawards, during high and low tides from September 1978 to September 1979. A total of 139 phytoplankters were encountered. Diatoms and dinoflagellates showed highest diversity in most saline areas. Typical freshwater organisms were restricted to stations I and II where salinity was more reduced. Maximum cell densities were: 11,256,000 cells/1, 4,380,000 cells/1, 1,276,000 cells/1 and 1,035,000 cells/1, for stations I, II, III and IV, respectively. Sewage enrichments were probably responsible for the greatest values of the first two stations. However, in these stations the turbidity reduces light penetration, limiting phytoplankton growth particularly during the rainy season. The phytoflagellates (maximum of up to 4,874,000 cells/1 and the diatoms Thalassiosira spp (maximum of up to 9,262,000 cells/1 were dominant during the annual cycle. Other important taxa were Navicula spp, Cylindrotheca closterium, Paralia sulcata, Thalassionema nitzschioides and the dinoflagellate Protoperidinium spp. Seasonal variations of phytoplankton densities, dissolved oxygen, phosphate, water transparency, temperature, salinity and suspended material are also presented.

  6. Spatial distribution of polycyclic aromatic hydrocarbons in Terminalia catappa L. (Combretaceae) bark from a selected heavy road traffic area of Rio de Janeiro City, Brazil.

    Science.gov (United States)

    Pereira Netto, Annibal D; Barreto, Renata P; Moreira, Josino C; Arbilla, Graciela

    2007-04-01

    The levels of 21 polycyclic aromatic hydrocarbons (PAHs) with molecular weights between 128 Da (naphthalene) and 300 Da (coronene) were determined in Terminalia catappa L. (Combretaceae) bark. Tree bark samples were collected in the campus of Oswaldo Cruz Foundation, a green area located in a very intensive traffic area of Rio de Janeiro City, Brazil and about 10 km away from the city center. Samples were submitted to ultrasonic extraction with dichloromethane and analyzed by high-resolution gas chromatography-mass spectrometry. Individual PAH levels varied from 1.23 to 327 ng/g and phenanthrene, fluoranthene and pyrene predominated in all samples. Total PAH levels ranged from 242 to 1640 ng/g with a mean of 628 ng/g. The levels of total PAHs and of total carcinogenic PAHs showed not a clear dependence with tree location and distances to the main routes of that area. No correlation was observed also with PAH levels previously found in total suspended particulate thus indicating that in this area tree bark PAH levels cannot be used to assess their atmospheric levels. PMID:17010512

  7. Phenotypical and genotypical characterization of Bordetella pertussis strains isolated in São Paulo, Brazil, 1988-2002 Caracterização fenotípica e genética de cepas de Bordetella pertussis isoladas em São Paulo, Brasil, 1988-2002

    Directory of Open Access Journals (Sweden)

    Célia R. Gonçalves

    2007-04-01

    Full Text Available Whooping cough or pertussis was a major cause of childhood morbidity and mortality in the world until the introduction of a whole-cell vaccine in the 1940's. However, since the early 1980's whooping cough cases have increased in many countries, becoming an important problem of public health. This increase may be due to accuracy of laboratory diagnosis and reporting of the disease, a decline in immunity over time, demographic changes, and adaptation of the bacterial population to vaccine-induced immunity. The purpose of this study was to analyze phenotypically and genotypically a collection of 67 Bordetella pertussis isolates recovered during the period 1988-2002 in São Paulo State, Brazil to determine their characteristics and relatedness. All isolates were submitted to susceptibility testing to erythromycin, serotyping, and 56 isolates were analyzed by Pulsed Field Gel Electrophoresis (PFGE. All isolates were susceptible to erythromycin and the majority of them belonged to serotype 1,3. The 56 isolates were classified into 11 PFGE profiles according to the differences in banding patterns. Although more than 60% of the isolates were recovered from patients aged less than three months, almost 15% of them were isolated from adolescents/adults evidencing the increase in the incidence of pertussis among this group of age.A coqueluche ou pertussis foi a maior causa de morbidade e mortalidade infantil em todo o mundo até a introdução de uma vacina na década de 1940. Entretanto, desde a década de 1980, a coqueluche tornou-se, em muitos países , um importante problema de saúde pública. Este acontecimento pode ser atribuído à melhoria do diagnóstico laboratorial e da notificação da doença, declínio da imunidade no decorrer do tempo, mudanças demográficas ou adaptação da população bacteriana à imunidade induzida pela vacina. O objetivo deste estudo foi analisar as características fenotípicas e genotípicas de uma coleção de 67

  8. Monthly Distribution of Phlebotomine Sand Flies, and Biotic and Abiotic Factors Related to Their Abundance, in an Urban Area to Which Visceral Leishmaniasis Is Endemic in Corumbá, Brazil

    Science.gov (United States)

    Casaril, Aline Etelvina; Fernandes, Wagner Souza; Ravanelli, Michelle de Saboya; Paranhos Filho, Antônio Conceição; Oshiro, Elisa Teruya; de Oliveira, Alessandra Gutierrez

    2016-01-01

    The monthly distribution and abundance of sand flies are influenced by both biotic and abiotic factors. The present study aimed to evaluate the seasonal distribution of sand flies and the relation between their abundance and environmental parameters, including vegetation and climate. This study was conducted over a 2-year period (April 2012 to March 2014). Monthly distribution was evaluated through the weekly deployment of CDC light traps in the peridomicile area of 5 residences in an urban area of the municipality of Corumbá in the State of Mato Grosso do Sul, Brazil. Meteorological data were obtained from the Mato Grosso do Sul Center for Weather, Climate, and Water Resources. The spectral indices were calculated based on spatial resolution images (GeoEye) and the percentage of vegetal coverage. Differences in the abundance of sand flies among the collection sites were assessed using the Kruskal-Wallis test, and the strength of correlations between environmental variables was determined by calculating Spearman’s correlation coefficients. Lutzomyia cruzi, Lu. forattinii, and Evandromyia corumbaensis were the most frequently found species. Although no significant association was found among these sand fly species and the tested environmental variables (vegetation and climate), high population peaks were found during the rainy season, whereas low peaks were observed in the dry season. The monthly distribution of sand flies was primarily determined by Lu. cruzi, which accounted for 93.94% of the specimens collected each month throughout the experimental period. The fact that sand flies were detected year-round indicates a continuous risk of infection to humans, demonstrating the need for targeted management and education programs. PMID:27783667

  9. SNP genotyping technologies

    DEFF Research Database (Denmark)

    Studer, Bruno; Kölliker, Roland

    2013-01-01

    In the recent years, single nucleotide polymorphism (SNP) markers have emerged as the marker technology of choice for plant genetics and breeding applications. Besides the efficient technologies available for SNP discovery even in complex genomes, one of the main reasons for this is the availabil......In the recent years, single nucleotide polymorphism (SNP) markers have emerged as the marker technology of choice for plant genetics and breeding applications. Besides the efficient technologies available for SNP discovery even in complex genomes, one of the main reasons...... for this is the availability of high-throughput platforms for multiplexed SNP genotyping. Advancements in these technologies have enabled increased flexibility and throughput, allowing for the generation of adequate SNP marker data at very competitive cost per data point....

  10. The legacy of organochlorine pesticide usage in a tropical semi-arid region (Jaguaribe River, Ceará, Brazil): Implications of the influence of sediment parameters on occurrence, distribution and fate.

    Science.gov (United States)

    Oliveira, André H B; Cavalcante, Rivelino M; Duaví, Wersângela C; Fernandes, Gabrielle M; Nascimento, Ronaldo F; Queiroz, Maria E L R; Mendonça, Kamila V

    2016-01-15

    Between the 1940s and 1990s, immeasurable amounts of organochlorine pesticides (OCPs) were used in endemic disease control campaigns and agriculture in the tropical semi-arid regions of Brazil. The present study evaluated the legacy of banned OCP usage, considering the levels, ecological risk and dependence on sediment physicochemical properties for the fate and distribution in the Jaguaribe River. The sum concentration of OCPs (ΣOCPs) ranged from 5.09 to 154.43 ng·g(-1), comparable to the levels found in other tropical and subtropical regions that have traditionally used OCPs. The environmental and geographical distribution pattern of p,p-DDT, p,p-DDD and p,p-DDE shows that the estuarine zone contained more than 3.5 times the levels observed in the fluvial region, indicating that the estuary of the Jaguaribe River is a sink. The temporal pattern indicates application of dichloro-diphenyl-trichloroethanes (DDTs) in the past; however, there is evidence of recent input of these pesticides. High ecological risk was observed for levels of γ-hexachlorocyclohexanes (γ-HCH) and heptachlor, and moderate ecological risk was observed for levels of DDTs in sediments from the Jaguaribe River. The heptachlor, γ-HCH and hexachlorobenzene (HCB) concentrations depend on the organic and inorganic fractions of sediment from the Jaguaribe River, whereas the p,p-DDE, p,p-DDD, p,p-DDT and α-endosulfan concentrations depend solely on the organic fraction of the sediment.

  11. Isolation and identification of Acanthamoeba spp. from thermal swimming pools and spas in Southern Brazil.

    Science.gov (United States)

    Fabres, Laura Fuhrich; Rosa Dos Santos, Sayonara Peixoto; Benitez, Lisianne Brittes; Rott, Marilise Brittes

    2016-03-01

    Free-living amoebae (FLA) are widely distributed in soil and water. A few number of them are implicated in human disease: Acanthamoeba spp., Naegleria fowleri, Balamuthia mandrillaris and Sappinia diploidea. Species of Acanthamoeba can cause keratitis and brain infections. In this study, 72 water samples were taken from both hot tubs and thermal swimming pools in the city of Porto Alegre, RS, Brazil, to determine the presence of Acanthamoeba in the water as well as perform the phenotypic and genotypic characterization of the isolates. The identification of the isolates was based on the cysts morphology and PCR amplification using genus-specific oligonucleotides. When the isolates were submitted to PCR reaction only 8 were confirmed as belonging to the genus Acanthamoeba. The sequences analysis when compared to the sequences in the GenBank, showed genotype distribution in group T3 (12,5%), T5 (12,5%), T4 (25%) and T15 (50%). The results of this study confirmed the presence of potentially pathogenic isolates of free living amoebae in hot swimming pool and spas which can present risks to human health.

  12. Soroprevalência e genótipos do vírus da hepatite C em pacientes com lúpus eritematoso sistêmico (LES em Goiânia, Brasil Hepatitis C virus seroprevalence and genotypes in patients with systemic lupus erythematosus (SLE in Goiânia, Brazil

    Directory of Open Access Journals (Sweden)

    Vitalina de Souza Barbosa

    2005-08-01

    (HCV infection is a source of concern in rheumatology because of its extrahepatic manifestations. Many studies have reported association between HCV infection and rheumatological manifestations such as: musculoskeletal pain, essential mixed cryoglobulinemia, rheumatoid arthritis, Sjögren's syndrome, vasculitis, glomerulonephritis, Raynaud's phenomenon, polyarteritis nodosa, myositis, autoantibody and other connective tissue diseases. In previous studies developed in our region, prevalences of 0.9%, 1.4%, 1.8% and 2.0% were detected among pregnant women, blood donors, leprosy patients and health professionals, respectively. OBJECTIVE: to investigate the prevalence of hepatitis C virus infection among patients with systemic lupus erythematosus (SLE in Goiânia, Brazil. METHODS: 175 patients were interviewed and had blood samples tested for HCV antibodies (anti-HCV by a third generation enzyme linked immunosorbant assay (ELISA. RNA-HCV was detected by polymerase chain reaction (PCR with primers complementary the 5' non-coding region of the HCV genoma, in all anti-HCV positive serum samples and genotyped by a line probe assay. RESULTS: an overall HCV infection prevalence of 2.3% (4/175 was found. Genotyping of RNA-HCV positive samples revealed HCV type 1 in 3 (75% and type 3 in 1 (25% patient. Clinical course was favorable in all HCV positive patients, except one, who died due to renal insuficiency related to lupus nephritis. CONCLUSIONS: anti-HCV prevalence among patients with SLE was slitghly higher than the prevanlence observed in pregnant women, healthy blood donors and leprosy patients, and similar to health professionals.

  13. Hepatitis B and C in the hemodialysis unit of Tocantins, Brazil: serological and molecular profiles

    Directory of Open Access Journals (Sweden)

    Karla P Souza

    2003-07-01

    Full Text Available A survey was conducted in the hemodialysis population of the state of Tocantins, Brazil, aiming to assess the prevalence of hepatitis B virus (HBV and hepatitis C virus (HCV infections, to analyze associated risk factors, and also to investigate these viruses genotypes distribution. During January and March 2001, all patients (n = 100 were interviewed at the unique dialysis unit in Tocantins. Blood samples were collected and serum samples were screened for HBV serological markers. Hepatitis B surface antigen positive samples were tested for HBV DNA. All samples were also tested for anti-HCV antibodies and HCV RNA. An overall prevalence of 45% was found for HBV infection (4% were HBsAg/anti-HBc positive, 2% were anti-HBc only and 39% had anti-HBc/anti-HBs markers. Concerning HCV infection, anti-HCV and HCV RNA were detected in 13% and 14% of the subjects, respectively. Three patients were HCV RNA positive and anti-HCV negative, resulting in an overall HCV prevalence of 16%. Univariate analysis of risk factors showed that only shift and length of time on hemodialysis were associated with HBV and HCV positivity, respectively. Among the four HBsAg-positive samples, HBV DNA was detected in three of them, which were identified as genotype A by restriction fragment length polymorphism (RFLP analysis. All 14 HCV RNA-positive samples were genotyped by INNO-LiPA. Genotypes 1a and 3a were found in 85% and 15%, respectively. The present data show low HBsAg and HCV prevalence rates. The risk factors associated with HBV and HCV positivity suggest that nosocomial transmission may influence in spreading these viruses in the dialysis unit studied.

  14. Noise in Genotype Selection Model

    Institute of Scientific and Technical Information of China (English)

    AI Bao-Quan; CHEN Wei; WANG Xian-Ju; LIU Guo-Tao; WEN De-Hua; LIU Liang-Gang

    2003-01-01

    We study the steady state properties ofa genotype selection model in presence of correlated Gaussian whitenoise. The effect of the noise on the genotype selection model is discussed. It is found that correlated noise can breakthe balance of gene selection and induce the phase transition which can makes us select one type gene haploid from agene group.

  15. Concentrations and distributions of metals in tissues of stranded green sea turtles (Chelonia mydas) from the southern Atlantic coast of Brazil.

    Science.gov (United States)

    da Silva, Cinthia Carneiro; Varela, Antonio Sergio; Barcarolli, Indianara Fernanda; Bianchini, Adalto

    2014-01-01

    Silver (Ag), cadmium (Cd), copper (Cu), lead (Pb) and zinc (Zn) concentrations were analyzed in tissues of juvenile green sea turtles (Chelonia mydas) found stranded along the southern Atlantic coast in Brazil. Green sea turtles were collected (n=29), measured (curved carapace length: CCL) and had their muscle, liver, and kidney dissected for metal concentration measurements. Sex was identified in 18 individuals (10 females and 8 males) through gonad histology. No gender differences in CCL and tissue metal concentrations were observed. In the muscle, there was a negative correlation between CCL and Cd and Cu concentrations. Metal concentrations were lower in the muscle than in the liver and kidney. Zn concentration in the muscle was the highest of all metals analyzed (16.6 mg/kg). The kidney showed the highest concentrations of Pb, Cd and Zn (5.4, 28.3 and 54.3 mg/kg, respectively), while the liver had the highest values of Ag and Cu (0.8 and 100.9 mg/kg, respectively). Tissue Ag, Zn and Cd concentrations were similar to those found in green sea turtles from other regions while Cu and Pb values were elevated, likely due to the metal-rich water and sediment reported in the collection area. In the liver and kidney, concentrations of non-essential (Ag, Cd and Pb) and essential (Cu or Zn) metals were positively correlated, likely due to an induced metallothionein synthesis to protect tissue against the toxic effect of metals. This is the first study to report and correlate the concentrations of essential and non-essential metals in tissues of green sea turtles in the Brazilian southern Atlantic coast, an important feeding and developing area for this turtle species.

  16. Distribution of {sup 137}Cs in three representative soils of Pernambuco state, Brazil;Distribuicao de {sup 137}Cs em tres solos representativos do estado de Pernambuco

    Energy Technology Data Exchange (ETDEWEB)

    Antunes, Patrik Diogo, E-mail: pdantunes@yahoo.com.b [Universidade Federal Rural de Pernambuco (UFRPE), Recife, PE (Brazil). Dept. de Ciencias do Solo; Sampaio, Everardo Valadares de Sa Barretto; Ferreira Junior, Antonio Luiz Goncalves; Salcedo, Ignacio Hernan, E-mail: esampaio@ufpe.b, E-mail: antonio.ferreira@ariano.nlink.com.b, E-mail: salcedo@ufpe.b [Universidade Federal Rural de Pernambuco (UFRPE), Recife, PE (Brazil). Dept. de Energia Nuclear; Galindo, Izabel Cristina Luna, E-mail: iclgalindo@uol.com.b [Universidade Federal Rural de Pernambuco (UFRPE), Recife, PE (Brazil). Dept. de Agronomia

    2010-05-15

    Contents of {sup 137}Cs have been used to quantify soil loss or accumulations, based on values of reference sites with little soil disturbance in the last decades. In Northeastern Brazil no such sites have been evaluated yet. Three areas with flat topography, native vegetation and undisturbed soil were selected in of Goiania, Araripina and Sertania counties, state of Pernambuco. Four points in each area were marked at a distance of 20 to 50 m away from each other and soil samples were collected in 3 cm depth intervals, down to 30 cm. The {sup 137}Cs activities were determined using gamma spectrometry with a hyper pure germanium detector. Average contents of {sup 137}Cs (Bq m{sup -2}) were 71.6 +- 6.3 in Goiania, 64.0 +- 13.8 in Araripina and 95.5 +- 9.8 in Sertania. Values in this range have been reported in Paraiba and Bahia, and higher ones in the Southeastern region, confirming the reduction of stocks in areas closer to the equator. The highest activities occurred in the surface layers, ranging from 0.5 to 1.6 Bq kg{sup -1}, and decreased linearly with depth until the detection limits: 18 cm in Araripina, 15 cm in Goiania, and 9 cm in Sertania. In Goiania, the activity was highest (2.06 +- 1.0 Bq kg{sup -1}) in an organic horizon above a inorganic horizon. The high activities and stock and lower depth in Sertania could be explained by the predominance of 2:1 clay minerals versus 1:1 clay minerals in the other areas. {sup 137}Cs activities were positively correlated with water and KCl pH values and negatively correlated with Al concentrations. The results confirm that, in the reference areas, {sup 137}Cs activities are highest in the surface layers, decreasing regularly down the soil profile. (author)

  17. Distribution of palaeosols and deposits in the temporal evolution of a semiarid fluvial distributary system (Bauru Group, Upper Cretaceous, SE Brazil)

    Science.gov (United States)

    Basilici, Giorgio; Bo, Patrick Führ Dal'; de Oliveira, Emerson Ferreira

    2016-07-01

    The stratigraphic and sedimentological knowledge of the Bauru Group (Upper Cretaceous, SE Brazil) is still generally insufficient and controversial. A sedimentological and palaeopedological study allowed to interpret the south-eastern portion of the Bauru Group according to the model of a fluvial distributary system. This work has two objectives: (1) to include palaeosols in the interpretation of a fluvial distributary system and (2) to give detailed information on the sedimentological and stratigraphic features of the SE portion of the Bauru Group in order to support biostratigraphical, taphonomic and palaeoecological studies. In the south-eastern portion of the Bauru Group, three genetic stratigraphic units were described and interpreted, here informally called lower, intermediate and upper units. The lower unit is constituted of muddy sandstone salt flat deposits and sandstone sheet deltas deposits and is interpreted as a basinal part of a fluvial distributary system. The intermediate unit is formed of very fine to fine-grained sandstone-filled ribbon channel and sandy sheet-shaped beds, suggesting a distal or medial portion of a fluvial distributary system. The upper unit does not match with the present models of the fluvial distributary system because mostly constituted of moderately developed, well-drained, medium- to fine-grained sandstone palaeosols, which testify pauses of sedimentation to the order of 104 years. Preserved features of sedimentary structures suggest that the parent material was formed by occasional catastrophic unconfined flows. This unit may represent the most distal portion of a fluvial distributary system generated by retrogradation of the alluvial system due to aridification of the climate. The upper unit may be interpreted also as proximal portion of fluvial distributary system if considering the coarser-grained and the well-drained palaeosols. However, the absence of channel deposits makes this interpretation unconvincing.

  18. Offshore distribution patterns of the cyanobacterium Trichodesmium erythraeum ehrenberg and associated phyto- and bacterioplankton in the southern Atlantic coast (Paraná, Brazil

    Directory of Open Access Journals (Sweden)

    Adriana Siqueira

    2006-03-01

    Full Text Available Studies were carried out on Thrichodesmium erythraeum occurring on the inner shelf in the state of Paraná, Brazil. Temperature, salinity, rainfall, wind velocity, total bacteria, bacterial biomass, chlorophyll-a, phytoplankton, Anabaena sp., Merismopedia sp. and T. erythraeum densities were measured in surface water. Centric and pennate diatoms, Anabaena sp. and Merismopedia sp. were most abundant at 15 m isobath, while dinoflagellate abundance was relatively constant among stations. Similarly, total bacterial densities were relatively homogeneous throughout the sampling area, suggesting that blooms of T. erythraeum were not yet in the senescent phase. Results showed that T. erythraeum was capable of surviving in relatively inhospitable environmental conditions, due to its ability to fix nitrogen and to photosynthesis at high light intensities.O propósito principal da presente pesquisa foi investigar as florações de Trichodesmium erythraeum na plataforma continental interna do Estado do Paraná, Brasil. Foram medidas, em águas de superfície a temperatura, salinidade, bactérias totais, biomassa bacteriana, clorofila-a, densidade fitoplanctônica, densidade das cianobactérias Anabaena sp., Merismopedia sp. e T. erythraeum. Ao contrário dos dinoflagelados, cuja abundância foi relativamente constante em todas as estações, as diatomáceas cêntricas e penadas, Anabaena sp. e Merismopedia sp. foram mais abundantes até a isóbata dos 15 m. A densidade de bactérias totais também foi relativamente homogênea na área amostrada, o que sugere que as florações de T. erythraeum não se encontravam em fase senescente. Os resultados confirmam que T. erythraeum é capaz de sobreviver em condições ambientais relativamente inóspitas devido à sua capacidade de fixar nitrogênio e efetuar a fotossíntese em altas intensidades de luz.

  19. 新疆石河子地区HPV感染的基因型分布特点%The genotype distribution characteristics of HPV infection in Shihezi region of Xinjiang

    Institute of Scientific and Technical Information of China (English)

    苟永慧; 廖新华; 程江

    2015-01-01

    Objective:To investigate the epidemic status and infection subtype distribution of HPV in cervical tissue in Shihezi area of Xinjiang,in order to provide the basis for development of HPV vaccine in this area.Methods:2 250 women were tested DNA genotyping of cervical exfoliated cells of HPV using liquid chip technology,and then statistically analyzed the infection rate of HPV and subtype distribution.Results:Among 2 250 women who were detected in the HPV,537 cases of DNA positive,the total positive rate was 23.87%.25 subtypes(HPV 73 type not found) were detected,in which high-risk subtype infection was 74.12%(398/537),low-risk subtype infection was 17.88% (96/537),mixed infection with high risk subtypes and low-risk subtype was 8.01% (43/537).The most common high-risk subtypes were HPV-16,39,and 55 type;the infection rates were 2.84% (64/2250), 2.49% (56/2250) and 2.13% (48/2250) respectively;the highest infection rate of subtype with low risk HPV were HPV-6 and HPV-11 respectively,while the infection rates were 2.27% (51/2250) and 1.69% (38/2250) respectively.Of with cervical HPV infection rate(33.64% ) and multiple HPV subtype infection rate(30.45% ) women of 25~35 years old were the highest,and differences of HPV infection rate in each age group were statistically significant(P<0.01).Conclusion:HPV subtypes in Shihezi area of Xinjiang is obviously different from other regions,which provides certain reference value to work for HPV vaccination and cervical cancer prevention and treatment in Shihezi area of Xinjiang.%目的:探讨新疆石河子地区宫颈组织中HPV的流行状况和感染亚型分布,为该地区HPV疫苗研制提供基础依据。方法:用液态芯片技术对2250例妇女进行宫颈脱落细胞HPV DNA基因分型检测,并对HPV感染率和亚型分布进行统计分析。结果:2250例妇女中共检出HPV DNA阳性537例,总检出率23.87%;共检出25种亚型(HPV 73型未检出),其中高危亚型感染74.12%(398/537)

  20. Genetic parameters and simultaneous selection for root yield, adaptability and stability of cassava genotypes

    Directory of Open Access Journals (Sweden)

    João Tomé de Farias Neto

    2013-12-01

    Full Text Available The objective of this work was to estimate genetic parameters and to evaluate simultaneous selection for root yield and for adaptability and stability of cassava genotypes. The effects of genotypes were assumed as fixed and random, and the mixed model methodology (REML/Blup was used to estimate genetic parameters and the harmonic mean of the relative performance of genotypic values (HMRPGV, for simultaneous selection purposes. Ten genotypes were analyzed in a complete randomized block design, with four replicates. The experiment was carried out in the municipalities of Altamira, Santarém, and Santa Luzia do Pará in the state of Pará, Brazil, in the growing seasons of 2009/2010, 2010/2011, and 2011/2012. Roots were harvested 12 months after planting, in all tested locations. Root yield had low coefficients of genotypic variation (4.25% and broad-sense heritability of individual plots (0.0424, which resulted in low genetic gain. Due to the low genotypic correlation (0.15, genotype classification as to root yield varied according to the environment. Genotypes CPATU 060, CPATU 229, and CPATU 404 stood out as to their yield, adaptability, and stability.

  1. Distribution of CYP2D6 Alleles and Phenotypes in the Brazilian Population

    Science.gov (United States)

    Sortica, Vinicius A.; Suarez-Kurtz, Guilherme; de Moraes, Maria Elizabete; Pena, Sergio D. J.; dos Santos, Ândrea K. Ribeiro; Romano-Silva, Marco A.; Hutz, Mara H.

    2014-01-01

    Abstract The CYP2D6 enzyme is one of the most important members of the cytochrome P450 superfamily. This enzyme metabolizes approximately 25% of currently prescribed medications. The CYP2D6 gene presents a high allele heterogeneity that determines great inter-individual variation. The aim of this study was to evaluate the variability of CYP2D6 alleles, genotypes and predicted phenotypes in Brazilians. Eleven single nucleotide polymorphisms and CYP2D6 duplications/multiplications were genotyped by TaqMan assays in 1020 individuals from North, Northeast, South, and Southeast Brazil. Eighteen CYP2D6 alleles were identified in the Brazilian population. The CYP2D6*1 and CYP2D6*2 alleles were the most frequent and widely distributed in different geographical regions of Brazil. The highest number of CYPD6 alleles observed was six and the frequency of individuals with more than two copies ranged from 6.3% (in Southern Brazil) to 10.2% (Northern Brazil). The analysis of molecular variance showed that CYP2D6 is homogeneously distributed across different Brazilian regions and most of the differences can be attributed to inter-individual differences. The most frequent predicted metabolic status was EM (83.5%). Overall 2.5% and 3.7% of Brazilians were PMs and UMs respectively. Genomic ancestry proportions differ only in the prevalence of intermediate metabolizers. The IM predicted phenotype is associated with a higher proportion of African ancestry and a lower proportion of European ancestry in Brazilians. PM and UM classes did not vary among regions and/or ancestry proportions therefore unique CYP2D6 testing guidelines for Brazilians are possible and could potentially avoid ineffective or adverse events outcomes due to drug prescriptions. PMID:25329392

  2. Distribution of CYP2D6 alleles and phenotypes in the Brazilian population.

    Directory of Open Access Journals (Sweden)

    Deise C Friedrich

    Full Text Available The CYP2D6 enzyme is one of the most important members of the cytochrome P450 superfamily. This enzyme metabolizes approximately 25% of currently prescribed medications. The CYP2D6 gene presents a high allele heterogeneity that determines great inter-individual variation. The aim of this study was to evaluate the variability of CYP2D6 alleles, genotypes and predicted phenotypes in Brazilians. Eleven single nucleotide polymorphisms and CYP2D6 duplications/multiplications were genotyped by TaqMan assays in 1020 individuals from North, Northeast, South, and Southeast Brazil. Eighteen CYP2D6 alleles were identified in the Brazilian population. The CYP2D6*1 and CYP2D6*2 alleles were the most frequent and widely distributed in different geographical regions of Brazil. The highest number of CYPD6 alleles observed was six and the frequency of individuals with more than two copies ranged from 6.3% (in Southern Brazil to 10.2% (Northern Brazil. The analysis of molecular variance showed that CYP2D6 is homogeneously distributed across different Brazilian regions and most of the differences can be attributed to inter-individual differences. The most frequent predicted metabolic status was EM (83.5%. Overall 2.5% and 3.7% of Brazilians were PMs and UMs respectively. Genomic ancestry proportions differ only in the prevalence of intermediate metabolizers. The IM predicted phenotype is associated with a higher proportion of African ancestry and a lower proportion of European ancestry in Brazilians. PM and UM classes did not vary among regions and/or ancestry proportions therefore unique CYP2D6 testing guidelines for Brazilians are possible and could potentially avoid ineffective or adverse events outcomes due to drug prescriptions.

  3. Genotyping of Brazilian Giardia duodenalis human axenic isolates

    Directory of Open Access Journals (Sweden)

    ST Coradi

    2011-01-01

    Full Text Available Giardia duodenalis is a complex species that comprises at least seven distinct genetic groups (A to G, but only genotypes A and B are known to infect humans and a wide variety of other mammals. Regardless of biological, biochemical and antigenic analysis, several isolates maintained in vitro were not genetically typed yet. So, in the present study, five Brazilian axenic isolates obtained from asymptomatic and symptomatic patients were typed in order to determine the major genetic groups to which the isolates belonged. DNA was extracted from axenic trophozoites, fragments of glutamate dehydrogenase (gdh and triosephosphate isomerase (tpi genes were amplified by PCR and the isolate genotyping was carried out using restriction fragment length polymorphism (RFLP and DNA sequencing for both genes. The results revealed that all isolates were assigned to genotype A at both analyzed loci. Indeed, DNA sequence analysis classified the four isolates obtained from asymptomatic individuals into subtype AII, while the isolate obtained from the symptomatic patient was typed as subtype AI. Despite of the limited number of isolates assessed, the findings presented herein provide interesting insights on the occurrence of Giardia genotypes in Brazil and hold the perspective for future molecular and epidemiological investigations.

  4. Preliminary report of hepatitis B virus genotype prevalence in Iran

    Institute of Scientific and Technical Information of China (English)

    Seyed-Moayed Alavian; Hossein Keyvani; Mahdi Rezai; Neda Ashayeri; Homa Mohammad Sadeghi

    2006-01-01

    AIM: To determine the prevalence of hepatitis B virus (HBV) genotypes in Iranian hepatitis B surface antigen (HBsAg) carriers, chronic hepatitis B and cirrhotic patients.METIHODS: A total of 109 HBsAg-positive patients were included in this study. HBV genotypes were determined by using INNO-LiPA methodology which is based on the reverse hybridization principle.RESULTS: The distribution of patients with different stages of liver disease was as follows: 95 (86.4%) chronic hepatitis, 11 (10%) liver cirrhosis, and 3 (2.7%)inactive carrier. Of the chronic hepatitis and liver cirrhosis patients, 26.4% were HBeAg-positive while 70% were HBeAg-negative. Genotype D was the only detected type found in all patients. CONCLUSION: Classifying HBV into genotypes has to be cost-effective and clinically relevant. Our study indicates that HBV genotype D prevails in the Mediterranean area, Near and Middle East, and South Asia. Continued efforts for understanding HBV genotype through international co-operation will reveal further virological differences of the genotypes and their clinical relevance.

  5. Analysis of the distribution of radiopharmaceuticals for nuclear medicine in Brazil; Analise da distribuicao de Radiofarmacos para servicos de Medicina Nuclear no Brasil

    Energy Technology Data Exchange (ETDEWEB)

    Kuahara, Lilian T.; Correa, Eduardo L.; Potiens, Maria P.A., E-mail: lilian547@hotmail.com [Instituto de Pesquisas Energeticas e Nucleares (IPEN/CNEN-SP), Sao Paulo, SP (Brazil)

    2013-07-01

    The objective of this study was to analyze the distribution of radiopharmaceuticals produced by Instituto de Pesquisas Energeticas e Nucleares (IPEN/CNEN), as part of a project to develop a methodology for control and calibration of activimeters used by these Nuclear Medicine Services. This survey was conducted using registry data of registered customers and, with bases in such information, we analyzed the number of clinics all over the country. Considering the distribution of radiopharmaceuticals and what the most used in 2011, there was a total of 365 clinics, and this distribution as follows: Southeast with 56%, South 18%, Northeast 15%, North 4%, and Midwest with 7%. Among the various radioisotopes provided 26 were sold and most in demand are the {sup 67}Ga, {sup 131}I and IPEN-tec (technetium generator)

  6. Distribution of natural gas: introduction of the interruptible segment in Sao Paulo, Brazil; Distribuicao de gas natural canalizado: introducao do segmento interruptivel em Sao Paulo

    Energy Technology Data Exchange (ETDEWEB)

    Paula, Claudio Paiva de [Agencia Reguladora de Saneamento e Energia do Estado de Sao Paulo (ARSESP), SP (Brazil)

    2008-07-01

    The principal objective of this paper is the better knowledge and evaluation of the natural gas distribution network interruptible branch. This new service can be appropriate for thermal power generation on flexible dispatch mode, as 'take or pay' contracts surplus jobs. The paper indicates no regulatory restraints in an interruptible network implantation and one can guess that the inexistence of contracts for this service resembles only lack of conjunctural opportunities for this job applications. These difficulties appear both in the supply and distribution branches of the gas business, which prefer flat contracts. The final conclusion is interruptible contracts can be an improvement on the distribution business and certainly can accommodate a suitable demand and supply volumes in the long-term gas market balance. (author)

  7. Biochemical Education in Brazil.

    Science.gov (United States)

    Vella, F.

    1988-01-01

    Described are discussions held concerning the problems of biochemical education in Brazil at a meeting of the Sociedade Brazileira de Bioquimica in April 1988. Also discussed are other visits that were made to universities in Brazil. Three major recommendations to improve the state of biochemistry education in Brazil are presented. (CW)

  8. Distributed Problem Based Learning in a Low Resources Setting: The design and evaluation of a problem-based, web-based curriculum for training family doctors in Brazil

    NARCIS (Netherlands)

    J.B.C. Tomaz (Cisne)

    2013-01-01

    markdownabstract__Abstract__ This thesis explored the use of distributed PBL in health professional education in a low-resources context. Three central questions were studied in this thesis: (a) Is distance education an acceptable educational strategy to train family health professionals in the Sta

  9. Brazil: public health genomics.

    Science.gov (United States)

    Castilla, E E; Luquetti, D V

    2009-01-01

    Brazil represents half of South America and one third of Latin America, having more than 186 million inhabitants. After China and India it is the third largest developing country in the world. The wealth is unequally distributed among the states and among the people. Brazil has a large and complex health care system. A Universal Public Health System (SUS: Sistema SPACEnico de Saúde) covers the medical expenses for 80% of the population. The genetic structure of the population is very complex, including a large proportion of tri- hybrid persons, genetic isolates, and a panmictic large majority. Genetic services are offered at 64 genetic centers, half of them public and free. Nationwide networks are operating for inborn errors of metabolism, oncogenetics, and craniofacial anomalies. The Brazilian Society of Medical Genetics (SBGM) has granted 120 board certifications since 1986, and 7 recognized residences in medical genetics are operating in the country. Three main public health actions promoted by the federal government have been undertaken in the last decade, ultimately aimed at the prevention of birth defects. Since 1999, birth defects are reported for all 3 million annual live births, several vaccination strategies aim at the eradication of rubella, and wheat and maize flours are fortified with folic acid. Currently, the government distributes over 2 million US dollars to finance 14 research projects aimed at providing the basis for the adequate prevention and care of genetics disorders through the SUS. Continuity of this proactive attitude of the government in the area of genomics in public health is desired. PMID:19023184

  10. Pediatric lymphomas in Brazil

    Directory of Open Access Journals (Sweden)

    Gabriela Gualco

    2010-01-01

    Full Text Available OBJECTIVE: This study provides the clinical pathological characteristics of 1301 cases of pediatric/adolescent lymphomas in patients from different geographic regions of Brazil. METHODS: A retrospective analyses of diagnosed pediatric lymphoma cases in a 10-year period was performed. We believe that it represents the largest series of pediatric lymphomas presented from Brazil. RESULTS: Non-Hodgkin lymphomas represented 68% of the cases, including those of precursor (36% and mature (64% cell origin. Mature cell lymphomas comprised 81% of the B-cell phenotype and 19% of the T-cell phenotype. Hodgkin lymphomas represented 32% of all cases, including 87% of the classical type and 13% of nodular lymphocyte predominant type. The geographic distribution showed 38.4% of the cases in the Southeast region, 28.7% in the Northeast, 16.1% in the South, 8.8% in the North, and 8% in the Central-west region. The distribution by age groups was 15-18 years old, 33%; 11-14 years old, 26%; 6-10 years old, 24%; and 6 years old or younger, 17%. Among mature B-cell lymphomas, most of the cases were Burkitt lymphomas (65%, followed by diffuse large B-cell lymphomas (24%. In the mature T-cell group, anaplastic large cell lymphoma, ALK-positive was the most prevalent (57%, followed by peripheral T-cell lymphoma, then not otherwise specified (25%. In the group of classic Hodgkin lymphomas, the main histological subtype was nodular sclerosis (76%. Nodular lymphocyte predominance occurred more frequently than in other series. CONCLUSION: Some of the results found in this study may reflect the heterogeneous socioeconomical status and environmental factors of the Brazilian population in different regions.

  11. Genetic variability in wild genotypes of Passiflora cincinnata based on RAPD markers.

    Science.gov (United States)

    Cerqueira-Silva, C B M; Conceição, L D H C S; Santos, E S L; Cardoso-Silva, C B; Pereira, A S; Oliveira, A C; Corrêa, R X

    2010-12-21

    The genetic diversity and characteristics of commercial interest of Passiflora species make it useful to characterize wild germplasm, because of their potential use for fruit, ornamental and medicinal purposes. We evaluated genetic diversity, using RAPD markers, of 32 genotypes of Passiflora cincinnata collected