WorldWideScience

Sample records for bravo pinus pinaster

  1. Vermicompost enhances germination of the maritime pine (Pinus pinaster Ait.)

    OpenAIRE

    Lazcano, Cristina; Sampedro, Luis; Zas Arregui, Rafael; Domínguez, Jorge

    2010-01-01

    We investigated the effect of vermicompost on the germination and early development of six different progenies of the maritime pine (Pinus pinaster Ait.). We compared the effects of incorporating solid vermicompost into the potting media to those of vermicompost water extract to asses the extent of not physically-mediated positive effects. The incorporation of vermicompost in the growing media of maritime pine increased germination by 16%, and particularly, addition of vermicom...

  2. A comparison of estimation methods for fitting Weibull, Johnson's SB and beta functions to Pinus pinaster, Pinus radiata and Pinus sylvestris stands in northwest Spain

    Energy Technology Data Exchange (ETDEWEB)

    Gorgoseo, J. J.; Rojo, A.; Camara-Obregon, A.; Dieguez-Aranda, U.

    2012-07-01

    The purpose of this study was to compare the accuracy of the Weibull, Johnson's SB and beta distributions, fitted with some of the most usual methods and with different fixed values for the location parameters, for describing diameter distributions in even-aged stands of Pinus pinaster, Pinus radiata and Pinus sylvestris in northwest Spain. A total of 155 permanent plots in Pinus sylvestris stands throughout Galicia, 183 plots in Pinus pinaster stands throughout Galicia and Asturias and 325 plots in Pinus radiata stands in both regions were measured to describe the diameter distributions. Parameters of the Weibull function were estimated by Moments and Maximum Likelihood approaches, those of Johnson's SB function by Conditional Maximum Likelihood and by Knoebel and Burkhart's method, and those of the beta function with the method based on the moments of the distribution. The beta and the Johnson's SB functions were slightly superior to Weibull function for Pinus pinaster stands; the Johnson's SB and beta functions were more accurate in the best fits for Pinus radiata stands, and the best results of the Weibull and the Johnson's SB functions were slightly superior to beta function for Pinus sylvestris stands. However, the three functions are suitable for this stands with an appropriate value of the location parameter and estimation of parameters method. (Author) 44 refs.

  3. Chemical composition and antimicrobial activity of the essential oils of Pinus pinaster

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    Nouara Ait Mimoune

    2013-08-01

    Full Text Available Objective: To investigate the antimicrobial activity and chemical composition of essential oils of Pinus pinaster. Methods: Essential oils were extracted from the needles by hydrodistillation. The chemical composition of the obtained essential oils was analyzed using GC-MS technique. The antimicrobial potential has been tested against six microorganisms performing the disc diffusion assay. Results: Twenty-three components have been identified. β-caryophyllene (30.9% and β-selinene (13.45% were predominant compounds. The essential oil exhibited a moderate activity against Staphylococcus aureus, Bacillus subtilis and Escherichia coli, but did not affect the growth of Erwinia amylovora. Aspergillus flavus and Aspergillus niger were not inhibited by maritime pine essential oils. Conclusions: The essential oils from Pinus pinaster can be used as an antibacterial agent.

  4. Chemical composition and antimicrobial activity of the essential oils of Pinus pinaster

    Institute of Scientific and Technical Information of China (English)

    Nouara Ait Mimoune; Djouher Ait Mimoune; Aziza Yataghene

    2013-01-01

    Objective: To investigate the antimicrobial activity and chemical composition of essential oils ofPinus pinaster. Methods: Essential oils were extracted from the needles by hydrodistillation. The chemical composition of the obtained essential oils was analyzed using GC-MS technique. The antimicrobial potential has been tested against six microorganisms performing the disc diffusion assay.Results:Twenty-three components have been identified. β-caryophyllene (30.9%) and β-selinene (13.45%) were predominant compounds. The essential oil exhibited a moderate activity againstStaphylococcus aureus, Bacillus subtilis and Escherichia coli, but did not affect the growth of Erwinia amylovora. Aspergillus flavus and Aspergillus niger were not inhibited by maritime pine essential oils.Conclusions:The essential oils from Pinus pinaster can be used as an antibacterial agent.

  5. Estudio de potenciales alelopáticos originados por Eucalyptus globulus Labill., Pinus pinaster Ait. y Pinus radiata D.

    OpenAIRE

    Ballester, A.; Arias, A. M.; Cobián, B.; López Calvo, E.; Vieitez, E.

    2011-01-01

    Se ha estudiado el potencial alelopático de Eucalyptus globulus Labill., Pinus pinaster Ait. y Pinus radiata D. sobre el crecimiento y la germinación de diferentes especies herbáceas. Extractos acuosos de hojas y acículas recogidas en los meses de enero y abril inhiben fundamentalmente la germinación de las semillas de festuca, siendo la acción más importante en el mes de abril que en el de enero. El contacto directo entre hojas y acículas y las semillas a ensayar produce una inhibición muy f...

  6. The effects of fire severity on ectomycorrhizal colonization and morphometric features in Pinus pinaster Ait. seedlings

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    Pablo Vásquez-Gassibe

    2016-04-01

    Full Text Available Aim of the study: Mycorrhizal fungi in Mediterranean forests play a key role in the complex process of recovery after wildfires. A broader understanding of an important pyrophytic species as Pinus pinaster and its fungal symbionts is thus necessary for forest restoration purposes. This study aims to assess the effects of ectomycorrhizal symbiosis on maritime pine seedlings and how fire severity affects fungal colonization ability.Area of study: Central Spain, in a Mediterranean region typically affected by wildfires dominated by Pinus pinaster, a species adapted to fire disturbance.Material and Methods: We studied P. pinaster root apexes from seedlings grown in soils collected one year after fire in undisturbed sites, sites moderately affected by fire and sites highly affected by fire. Natural ectomycorrhization was observed at the whole root system level as well as at two root vertical sections (0-10 cm and 10-20 cm. We also measured several morphometric traits (tap root length, shoot length, dry biomass of shoots and root/shoot ratio, which were used to test the influence of fire severity and soil chemistry upon them.Main results: Ectomycorrhizal colonization in undisturbed soils for total and separated root vertical sections was higher than in soils that had been affected by fire to some degree. Inversely, seedling vegetative size increased according to fire severity.Research highlights: Fire severity affected soil properties and mycorrhizal colonization one year after occurrence, thus affecting plant development. These findings can contribute to a better knowledge of the factors mediating successful establishment of P. pinaster in Mediterranean forests after wildfires. 

  7. Desarrollo de un Adsorbente Basado en Taninos de Corteza de Pinus pinaster Development of an Adsorbent Based on Tannins from Pinus pinaster Bark

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    G. Vázquez

    2005-01-01

    Full Text Available Se han preparado partículas de gel de taninos de corteza de Pinus pinaster empleando un proceso en dos etapas. En la primera etapa (de pre unión cruzada, los taninos reaccionan con formaldehído. En la segunda (de granulación, la disolución taninos-formaldehído parcialmente gelificada se dispersa sobre una mezcla de decalina y un agente tensoactivo no iónico. Se ha analizado la influencia de las condiciones de operación de ambas etapas sobre el rendimiento y características de las partículas obtenidas. Las partículas, ligeramente porosas y prácticamente esféricas, se han empleado para la adsorción de cadmio de disoluciones acuosas. Los espectros de dispersión de rayos X obtenidos tras la adsorción han confirmado la presencia de cadmio sobre la superficie de las partículas y, por tanto, su capacidad para la adsorción de cationes metálicos.Tannin-gel particles, based on tannins extracted from Pinus pinaster bark, have been prepared in a two-stage process. In the first stage (pre-crosslinking, tannins react with formaldehyde. In the second stage (granulation, the partially gelled tannin-formaldehyde solution is dispersed into a mixture of decalin and a non-ionic surfactant. The influence of operation conditions of both stages on the yield and characteristics of the particles has been analysed. The particles, slightly porous and nearly spherical, have been used to adsorb cadmium from aqueous solutions. X-ray energy dispersion spectra obtained after adsorption have confirmed the presence of cadmium on particle surface and, therefore, their capacity for metal cation adsorption.

  8. Fungal communities and fire associated to pinus pinaster in a mediterránean region

    OpenAIRE

    Vásquez Gassibe, Pablo

    2014-01-01

    El objetivo de esta investigación fue estudiar cómo las comunidades de hongos de bosques mediterráneos dominados por Pinus pinaster Ait. están influenciados por el fuego y las diferentes condiciones edafoclimáticas que caracterizan específicamente estas zonas. Para evaluar el efecto del fuego se analizó la sucesión de las comunidades de hongos después de un incendio en un ecosistema mediterráneo en el noroeste de España y se desarrollaron los primeros modelos simples de clasificación para ...

  9. Envelope-like retrotransposons in the plant kingdom: evidence of their presence in gymnosperms (Pinus pinaster).

    Science.gov (United States)

    Miguel, Célia; Simões, Marta; Oliveira, Maria Margarida; Rocheta, Margarida

    2008-11-01

    Retroviruses differ from retrotransposons due to their infective capacity, which depends critically on the encoded envelope. Some plant retroelements contain domains reminiscent of the env of animal retroviruses but the number of such elements described to date is restricted to angiosperms. We show here the first evidence of the presence of putative env-like gene sequences in a gymnosperm species, Pinus pinaster (maritime pine). Using a degenerate primer approach for conserved domains of RNaseH gene, three clones from putative envelope-like retrotransposons (PpRT2, PpRT3, and PpRT4) were identified. The env-like sequences of P. pinaster clones are predicted to encode proteins with transmembrane domains. These sequences showed identity scores of up to 30% with env-like sequences belonging to different organisms. A phylogenetic analysis based on protein alignment of deduced aminoacid sequences revealed that these clones clustered with env-containing plant retrotransposons, as well as with retrotransposons from invertebrate organisms. The differences found among the sequences of maritime pine clones isolated here suggest the existence of different putative classes of env-like retroelements. The identification for the first time of env-like genes in a gymnosperm species may support the ancestrality of retroviruses among plants shedding light on their role in plant evolution.

  10. Forest structure and plant diversity in maritime pine (Pinus pinaster Ait.) stands in central Spain

    Energy Technology Data Exchange (ETDEWEB)

    Osorio, L. F.; Bravo, F.; Zaldivar, P.; Pando, V.

    2009-07-01

    The relationship between forest structure and plant diversity in Mediterranean Maritime pine stands (Pinus pinaster Ait.) in the Iberian Range (Spain) was studied. Forty eight stands were sampled. In each, a circular plot (15 m radius) and a transect (25*1 m{sup 2}) were established to estimate stand variables and record presence and abundance of vascular species respectively. Canonical correlation analysis (CCA), simple correlations and multiple stepwise linear regressions were used to explore the relationship between plant diversity and forest structure. Correlation between diversity measurements and stand variables is very weak, but significant correlations were found when evaluating each set of variables separately. Presence and cover of some species (for instance, Veronica arvensis L. or Micropyrum tenellum (L.) Link) is correlated with stand variables; however, determination coefficients found in step-by-step regression are not significant. (Author) 34 refs.

  11. Adsorption of phenol on formaldehyde-pretreated Pinus pinaster bark: equilibrium and kinetics.

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    Vázquez, G; González-Alvarez, J; García, A I; Freire, M S; Antorrena, G

    2007-05-01

    This work studies phenol adsorption on Pinus pinaster bark that has been previously treated with formaldehyde in acid medium. The influence of several variables such as solid/liquid ratio, pH and initial concentration of phenol in the solution on the adsorption capacity of the bark has been analysed. A kinetic model based on phenol diffusion within the pores of the adsorbent was in agreement with the results obtained for high initial concentrations of phenol, allowing the determination of diffusion coefficients. Adsorption equilibrium data were fitted by the Freundlich and BET isotherms. From their parameters phenol adsorption capacity and intensity, as well as the specific surface (BET) of the adsorbent, were determined.

  12. Assessing wildfire occurrence probability in Pinus pinaster Ait. stands in Portugal

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    Marques, S.; Garcia-Gonzalo, J.; Botequim, B.; Ricardo, A.; Borges, J. G.; Tome, M.; Oliveira, M. M.

    2012-11-01

    Maritime pine (Pinus pinaster Ait.) is an important conifer from the western Mediterranean Basin extending over 22% of the forest area in Portugal. In the last three decades nearly 4% of Maritime pine area has been burned by wildfires. Yet no wildfire occurrence probability models are available and forest and fire management planning activities are thus carried out mostly independently of each other. This paper presents research to address this gap. Specifically, it presents a model to assess wildfire occurrence probability in regular and pure Maritime pine stands in Portugal. Emphasis was in developing a model based on easily available inventory data so that it might be useful to forest managers. For that purpose, data from the last two Portuguese National Forest Inventories (NFI) and data from wildfire perimeters in the years from 1998 to 2004 and from 2006 to 2007 were used. A binary logistic regression model was build using biometrics data from the NFI. Biometric data included indicators that might be changed by operations prescribed in forest planning. Results showed that the probability of wildfire occurrence in a stand increases in stand located at steeper slopes and with high shrubs load while it decreases with precipitation and with stand basal area. These results are instrumental for assessing the impact of forest management options on wildfire probability thus helping forest managers to reduce the risk of wildfires. (Author) 57 refs.

  13. Water availability and branch length determine delta(13)C in foliage of Pinus pinaster.

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    Warren, Charles R.; Adams, Mark A.

    2000-05-01

    The stable carbon isotope composition (delta(13)C) of foliage integrates signals resulting from environmental and hydraulic constraints on water movement and photosynthesis. We used branch length as a simple predictor of hydraulic constraints to water fluxes and determined the response of delta(13)C to varying water availability. Foliage up to 6 years old was taken from Pinus pinaster Ait. trees growing at four sites differing in precipitation (P; 414-984 mm year(-1)) and potential evaporation (ET; 1091-1750 mm year(-1)). Branch length was the principal determinant of temporal trends in delta(13)C. The strong relationship between delta(13)C and branch length was a function of hydraulic conductance, which was negatively correlated with branch length (r(2) = 0.84). Variation in P and ET among sites was reflected in delta(13)C, which was negatively correlated with P/ET (r(2) = 0.66). However, this analysis was confounded by differences in branch length. If the effects of branch length on delta(13)C were first removed, then the 'residual' delta(13)C was more closely related to P/ET (r(2) = 0.99), highlighting the importance of accounting for variation in hydraulic constraints to water flux between sites and years. For plant species that exhibit considerable phenotypic plasticity in response to changes in environment (e.g., variation in leaf area, branch length and number, or stem form), the environmental effects on delta(13)C in foliage can only be reliably assessed if deconvoluted from hydraulic constraints.

  14. Adaptive potential of maritime pine (Pinus pinaster populations to the emerging pitch canker pathogen, Fusarium circinatum.

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    Margarita Elvira-Recuenco

    Full Text Available There is a concern on how emerging pests and diseases will affect the distribution range and adaptability of their host species, especially due to different conditions derived from climate change and growing globalization. Fusarium circinatum, which causes pitch canker disease in Pinus species, is an exotic pathogen of recent introduction in Spain that threatens its maritime pine (P. pinaster stands. To predict the impact this disease will have on the species, we examine host resistance traits and their genetic architecture. Resistance phenotyping was done in a clonal provenance/progeny trial, using three-year-old cuttings artificially inoculated with the pathogen and maintained under controlled environmental conditions. A total number of 670 ramets were assessed, distributed in 10 populations, with a total of 47 families, 2 to 5 half-sibs per family, and 3-7 ramets per clone. High genetic variation was found at the three hierarchical levels studied: population, family and clone, being both additive and non-additive effects important. Narrow-sense and broad-sense heritability estimates were relatively high, with respective values of 0.43-0.58 and 0.51-0.8, depending on the resistance traits measured (lesion length, lesion length rate, time to wilting, and survival. These values suggest the species' high capacity of evolutionary response to the F. circinatum pathogen. A population originated in Northern Spain was the most resistant, while another from Morocco was the most susceptible. The total number of plants that did not show lesion development or presented a small lesion (length<30 mm was 224 out of 670, indicating a high proportion of resistant trees in the offspring within the analyzed populations. We found large differences among populations and considerable genetic variation within populations, which should allow, through natural or artificial selection, the successful adaptation of maritime pine to pitch canker disease.

  15. Incorporating exposure to pitch canker disease to support management decisions of Pinus pinaster Ait. in the face of climate change

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    Serra-Varela, María Jesús; Alía, Ricardo; Pórtoles, Javier; Gonzalo, Julián; Soliño, Mario; Grivet, Delphine; Raposo, Rosa

    2017-01-01

    Climate change is gravely affecting forest ecosystems, resulting in large distribution shifts as well as in increasing infection diseases and biological invasions. Accordingly, forest management requires an evaluation of exposure to climate change that should integrate both its abiotic and biotic components. Here we address the implications of climate change in an emerging disease by analysing both the host species (Pinus pinaster, Maritime pine) and the pathogen’s (Fusarium circinatum, pitch canker) environmental suitability i.e. estimating the host’s risk of habitat loss and the disease`s future environmental range. We constrained our study area to the Spanish Iberian Peninsula, where accurate climate and pitch canker occurrence databases were available. While P. pinaster is widely distributed across the study area, the disease has only been detected in its north-central and north-western edges. We fitted species distribution models for the current distribution of the conifer and the disease. Then, these models were projected into nine Global Climate Models and two different climatic scenarios which totalled to 18 different future climate predictions representative of 2050. Based on the level of agreement among them, we created future suitability maps for the pine and for the disease independently, which were then used to assess exposure of current populations of P. pinaster to abiotic and biotic effects of climate change. Almost the entire distribution of P. pinaster in the Spanish Iberian Peninsula will be subjected to abiotic exposure likely to be driven by the predicted increase in drought events in the future. Furthermore, we detected a reduction in exposure to pitch canker that will be concentrated along the north-western edge of the study area. Setting up breeding programs is recommended in highly exposed and productive populations, while silvicultural methods and monitoring should be applied in those less productive, but still exposed, populations

  16. Effects of Recent Minimum Temperature and Water Deficit Increases on Pinus pinaster Radial Growth and Wood Density in Southern Portugal.

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    Kurz-Besson, Cathy B; Lousada, José L; Gaspar, Maria J; Correia, Isabel E; David, Teresa S; Soares, Pedro M M; Cardoso, Rita M; Russo, Ana; Varino, Filipa; Mériaux, Catherine; Trigo, Ricardo M; Gouveia, Célia M

    2016-01-01

    Western Iberia has recently shown increasing frequency of drought conditions coupled with heatwave events, leading to exacerbated limiting climatic conditions for plant growth. It is not clear to what extent wood growth and density of agroforestry species have suffered from such changes or recent extreme climate events. To address this question, tree-ring width and density chronologies were built for a Pinus pinaster stand in southern Portugal and correlated with climate variables, including the minimum, mean and maximum temperatures and the number of cold days. Monthly and maximum daily precipitations were also analyzed as well as dry spells. The drought effect was assessed using the standardized precipitation-evapotranspiration (SPEI) multi-scalar drought index, between 1 to 24-months. The climate-growth/density relationships were evaluated for the period 1958-2011. We show that both wood radial growth and density highly benefit from the strong decay of cold days and the increase of minimum temperature. Yet the benefits are hindered by long-term water deficit, which results in different levels of impact on wood radial growth and density. Despite of the intensification of long-term water deficit, tree-ring width appears to benefit from the minimum temperature increase, whereas the effects of long-term droughts significantly prevail on tree-ring density. Our results further highlight the dependency of the species on deep water sources after the juvenile stage. The impact of climate changes on long-term droughts and their repercussion on the shallow groundwater table and P. pinaster's vulnerability are also discussed. This work provides relevant information for forest management in the semi-arid area of the Alentejo region of Portugal. It should ease the elaboration of mitigation strategies to assure P. pinaster's production capacity and quality in response to more arid conditions in the near future in the region.

  17. Adaptive consequences of human-mediated introgression for indigenous tree species: the case of a relict Pinus pinaster population.

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    Ramírez-Valiente, José Alberto; Robledo-Arnuncio, Juan José

    2014-12-01

    Human-induced gene movement via afforestation and restoration programs is a widespread phenomenon throughout the world. However, its effects on the genetic composition of native populations have received relatively little attention, particularly in forest trees. Here, we examine to what extent gene flow from allochthonous plantations of Pinus pinaster Aiton impacts offspring performance in a neighboring relict natural population and discuss the potential consequences for the long-term genetic composition of the latter. Specifically, we conducted a greenhouse experiment involving two contrasting watering treatments to test for differences in a set of functional traits and mortality rates between P. pinaster progenies from three different parental origins: (i) local native parents, (ii) exotic parents and (iii) intercrosses between local mothers and exotic fathers (intraspecific hybrids). Our results showed differences among crosses in cumulative mortality over time: seedlings of exotic parents exhibited the lowest mortality rates and seedlings of local origin the highest, while intraspecific hybrids exhibited an intermediate response. Linear regressions showed that seedlings with higher water-use efficiency (WUE, δ(13)C) were more likely to survive under drought stress, consistent with previous findings suggesting that WUE has an important role under dry conditions in this species. However, differences in mortality among crosses were only partially explained by WUE. Other non-measured traits and factors such as inbreeding depression in the relict population are more likely to explain the lower performance of native progenies. Overall, our results indicated that intraspecific hybrids and exotic individuals are more likely to survive under stressful conditions than local native individuals, at least during the first year of development. Since summer drought is the most important demographic and selective filter affecting tree establishment in Mediterranean ecosystems

  18. Determining mode I cohesive law of Pinus pinaster by coupling double cantilever beam test with digital image correlation

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    J. Xavier

    2015-01-01

    Full Text Available The direct identification of the cohesive law in pure mode I of Pinus pinaster is addressed. The approach couples the double cantilever beam (DCB test with digital image correlation (DIC. Wooden beam specimens loaded in the radial-longitudinal (RL fracture propagation system are used. The strain energy release rate in mode I ( is uniquely determined from the load-displacement ( curve by means of the compliance-based beam method (CBBM. This method relies on the concept of equivalent elastic crack length ( and therefore does not require the monitoring of crack propagation during test. The crack tip opening displacement in mode I is determined from the displacement field at the initial crack tip. The cohesive law in mode I is then identified by numerical differentiation of the relationship. Moreover, the proposed procedure is validated by finite element analyses including cohesive zone modelling. It is concluded that the proposed data reduction scheme is adequate for assessing the cohesive law in pure mode I of P. pinaster

  19. Efficient preparation of catechin thio conjugates by one step extraction/depolymerization of pine (Pinus pinaster) bark procyanidins.

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    Selga, Ariadna; Torres, Josep Lluís

    2005-10-05

    The skin penetrating antioxidant cysteamine derivative of (-)-epicatechin as well as other thio conjugates were efficiently obtained with high yields from pine (Pinus pinaster) bark by simultaneous one pot extraction and depolymerization using water and cysteamine hydrochloride. The influence of the concentration of bark, acid, and cysteamine, as well as the reaction time on the total conversion, was studied. The total conversion into the epicatechin and catechin conjugates was as high as 47 g/kg pine bark with 1666 g cysteamine/kg bark and 28 g/kg with 166 g cysteamine/kg bark. A fast cleanup step by absorption/desorption on XAD-16 greatly facilitated further purification of the active major component. At a pilot scale, 4beta-(2-aminoethylthio)epicatechin (1) (conversion 263 g, purity 35% by reversed phase high-performance liquid chromatography/weight) was obtained from 17 kg of pine bark after simultaneous extraction/depolymerization followed by cleanup with the polymeric resin in approximately 10 h. The results show that pine (P. pinaster) bark is a suitable source of flavanols for the preparation of active thio derivatives. Conditions are given for the fast and efficient preparation of the conjugates.

  20. Environmental influences on post-harvest natural regeneration of Pinus pinaster Ait. in Mediterranean forest stands submitted to seed-tree selection method

    OpenAIRE

    Rodríguez García, Encarna; Juez, Libertad; Bravo Oviedo, Felipe

    2010-01-01

    Research on natural regeneration is crucial for the development of sustainable forestry practices, in light of the global climate changes taking place. In this study, 151 plots were sampled in six Pinus pinaster stands that were naturally regenerated by the seed-tree method in Mediterranean forests in central Spain. The objectives of the survey were to study the suitability of different forest stands designated for natural regeneration as well as to analyse seedling establishment and the rela...

  1. Plastic Response of Tracheids in Pinus pinaster in a Water-Limited Environment: Adjusting Lumen Size instead of Wall Thickness

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    Carvalho, Ana; Nabais, Cristina; Vieira, Joana; Rossi, Sergio; Campelo, Filipe

    2015-01-01

    The formation of wood results from cambial activity and its anatomical properties reflect the variability of environmental conditions during the growing season. Recently, it was found that wood density variations in conifers growing under cold-limited environment result from the adjustment of cell wall thickness (CWT) to temperature. Additionally, it is known that intra-annual density fluctuations (IADFs) are formed in response to precipitation after the summer drought. Although IADFs are frequent in Mediterranean conifers no study has yet been conducted to determine if these structures result from the adjustment of lumen diameter (LD) or CWT to soil water availability. Our main objective is to investigate the intra-ring variation of wood anatomical features (LD and CWT) in Pinus pinaster Ait. growing under a water-limited environment. We compared the tracheidograms of LD and CWT for the years 2010–2013 in P. pinaster growing in the west coast of Portugal. Our results suggest a close association between LD and soil moisture content along the growing season, reinforcing the role of water availability in determining tracheid size. Compared with CWT, LD showed a higher intra- and inter-annual variability suggesting its strong adjustment value to variations in water availability. The formation of a latewood IADF appears to be predisposed by higher rates of cell production in spring and triggered by early autumn precipitation. Our findings reinforce the crucial role of water availability on cambial activity and wood formation in Mediterranean conifers, and emphasize the high plasticity of wood anatomical features under Mediterranean climate. PMID:26305893

  2. Plastic Response of Tracheids in Pinus pinaster in a Water-Limited Environment: Adjusting Lumen Size instead of Wall Thickness.

    Science.gov (United States)

    Carvalho, Ana; Nabais, Cristina; Vieira, Joana; Rossi, Sergio; Campelo, Filipe

    2015-01-01

    The formation of wood results from cambial activity and its anatomical properties reflect the variability of environmental conditions during the growing season. Recently, it was found that wood density variations in conifers growing under cold-limited environment result from the adjustment of cell wall thickness (CWT) to temperature. Additionally, it is known that intra-annual density fluctuations (IADFs) are formed in response to precipitation after the summer drought. Although IADFs are frequent in Mediterranean conifers no study has yet been conducted to determine if these structures result from the adjustment of lumen diameter (LD) or CWT to soil water availability. Our main objective is to investigate the intra-ring variation of wood anatomical features (LD and CWT) in Pinus pinaster Ait. growing under a water-limited environment. We compared the tracheidograms of LD and CWT for the years 2010-2013 in P. pinaster growing in the west coast of Portugal. Our results suggest a close association between LD and soil moisture content along the growing season, reinforcing the role of water availability in determining tracheid size. Compared with CWT, LD showed a higher intra- and inter-annual variability suggesting its strong adjustment value to variations in water availability. The formation of a latewood IADF appears to be predisposed by higher rates of cell production in spring and triggered by early autumn precipitation. Our findings reinforce the crucial role of water availability on cambial activity and wood formation in Mediterranean conifers, and emphasize the high plasticity of wood anatomical features under Mediterranean climate.

  3. "Contrasting patterns of selection at Pinus pinaster Ait. Drought stress candidate genes as revealed by genetic differentiation analyses".

    Science.gov (United States)

    Eveno, Emmanuelle; Collada, Carmen; Guevara, M Angeles; Léger, Valérie; Soto, Alvaro; Díaz, Luis; Léger, Patrick; González-Martínez, Santiago C; Cervera, M Teresa; Plomion, Christophe; Garnier-Géré, Pauline H

    2008-02-01

    The importance of natural selection for shaping adaptive trait differentiation among natural populations of allogamous tree species has long been recognized. Determining the molecular basis of local adaptation remains largely unresolved, and the respective roles of selection and demography in shaping population structure are actively debated. Using a multilocus scan that aims to detect outliers from simulated neutral expectations, we analyzed patterns of nucleotide diversity and genetic differentiation at 11 polymorphic candidate genes for drought stress tolerance in phenotypically contrasted Pinus pinaster Ait. populations across its geographical range. We compared 3 coalescent-based methods: 2 frequentist-like, including 1 approach specifically developed for biallelic single nucleotide polymorphisms (SNPs) here and 1 Bayesian. Five genes showed outlier patterns that were robust across methods at the haplotype level for 2 of them. Two genes presented higher F(ST) values than expected (PR-AGP4 and erd3), suggesting that they could have been affected by the action of diversifying selection among populations. In contrast, 3 genes presented lower F(ST) values than expected (dhn-1, dhn2, and lp3-1), which could represent signatures of homogenizing selection among populations. A smaller proportion of outliers were detected at the SNP level suggesting the potential functional significance of particular combinations of sites in drought-response candidate genes. The Bayesian method appeared robust to low sample sizes, flexible to assumptions regarding migration rates, and powerful for detecting selection at the haplotype level, but the frequentist-like method adapted to SNPs was more efficient for the identification of outlier SNPs showing low differentiation. Population-specific effects estimated in the Bayesian method also revealed populations with lower immigration rates, which could have led to favorable situations for local adaptation. Outlier patterns are discussed

  4. Pyrolysis of Pinus pinaster in a two-stage gasifier: Influence of processing parameters and thermal cracking of tar

    Energy Technology Data Exchange (ETDEWEB)

    Fassinou, Wanignon Ferdinand; Toure, Siaka [Laboratoire d' Energie Solaire-UFR-S.S.M.T. Universite de Cocody, 22BP582 Abidjan 22 (Ivory Coast); Van de Steene, Laurent; Volle, Ghislaine; Girard, Philippe [CIRAD-Foret, TA 10/16, 73, avenue J.-F. Breton, 34398 Montpellier, Cedex 5 (France)

    2009-01-15

    A new two-stage gasifier with fixed-bed has recently been installed on CIRAD facilities in Montpellier. The pyrolysis and the gasifier units are removable. In order to characterise the pyrolysis products before their gasification, experiments were carried out, for the first time only with the pyrolysis unit and this paper deals with the results obtained. The biomass used is Pinus pinaster. The parameters investigated are: temperature, residence time and biomass flow rate. It has been found that increasing temperature and residence time improve the cracking of tars, gas production and char quality (fixed carbon rate more than 90%, volatile matter rate less than 4%). The increase of biomass flow rate leads to a bad char quality. The efficiency of tar cracking, the quality and the heating value of the charcoal and the gases, indicate that: temperature between 650 C and 750 C, residence time of 30 min, biomass flow rate between 10 and 15 kg/h should be the most convenient experimental conditions to get better results from the experimental device and from the biomass pyrolysis process. The kinetic study of charcoal generation shows that the pyrolysis process, in experimental conditions, is a first-order reaction. The kinetic parameters calculated are comparable with those found by other researchers. (author)

  5. Basic density, extractive content and moisture sorption properties of Pinus pinaster wood infected with the pinewood nematode, Bursaphelenchus xylophilus

    Institute of Scientific and Technical Information of China (English)

    Valeria Reva; Luı´s Fonseca; Jose´ L. Lousada; Isabel Abrantes; Anto´ nio R. Figueiredo; Domingos X. Viegas

    2015-01-01

    The pinewood nematode (PWN), Bursaphe-lenchus xylophilus, has become one of the most severe threats to pine forest worldwide. Nematodes, migrating through resin canals and feeding on the living cells, induce rapid metabolic changes in ray parenchyma cells, create cavitation areas, decrease xylem water content and oleo-resin exudation, and cause necrosis of parenchyma and cambial cells. This study focused on the impact of PWN infection on technological parameters of wood and evalu-ated the impact of anatomic and biochemical incidences of tree defense reactions on basic density, extractive content and moisture sorption properties of Pinus pinaster wood. Samples of infected and uninfected wood were studied. The presence of nematodes reduced wood basic density by 2% and decreased the total content of extractives in infected wood as compared with uninfected (5.98 and 8.90% of dry wood mass, respectively). Extractives in infected trees had inverse distribution along the trunk as compared with uninfected trees. The adsorption isotherms for infected and uninfected wood had similar positioning. We recorded differences (some statistically significant) in the equilibrium moisture content of infected and uninfected wood under varying environmental conditions. Despite the verified differences in wood basic density, extractive con-tent and moisture sorption properties, the overall conclu-sion is that the PWN had a slight impact on these characteristics of wood.

  6. A GIS-Based Multicriteria Evaluation for Aiding Risk Management Pinus pinaster Ait. Forests: A Case Study in Corsican Island, Western Mediterranean Region

    Science.gov (United States)

    Pasqualini, Vanina; Oberti, Pascal; Vigetta, Stéphanie; Riffard, Olivier; Panaïotis, Christophe; Cannac, Magali; Ferrat, Lila

    2011-07-01

    Forest management can benefit from decision support tools, including GIS-based multicriteria decision-aiding approach. In the Mediterranean region, Pinus pinaster forests play a very important role in biodiversity conservation and offer many socioeconomic benefits. However, the conservation of this species is affected by the increase in forest fires and the expansion of Matsucoccus feytaudi. This paper proposes a methodology based on commonly available data for assessing the values and risks of P. pinaster forests and to generating maps to aid in decisions pertaining to fire and phytosanitary risk management. The criteria for assessing the values (land cover type, legislative tools for biodiversity conservation, environmental tourist sites and access routes, and timber yield) and the risks (fire and phytosanitation) of P. pinaster forests were obtained directly or by considering specific indicators, and they were subsequently aggregated by means of GIS-based multicriteria analysis. This approach was tested on the island of Corsica (France), and maps to aid in decisions pertaining to fire risk and phytosanitary risk ( M. feytaudi) were obtained for P. pinaster forest management. Study results are used by the technical offices of the local administration— Corsican Agricultural and Rural Development Agency (ODARC)—for planning the conservation of P. pinaster forests with regard to fire prevention and safety and phytosanitary risks. The decision maker took part in the evaluation criteria study (weight, normalization, and classification of the values). Most suitable locations are given to target the public intervention. The methodology presented in this paper could be applied to other species and in other Mediterranean regions.

  7. Microwave-assisted extraction and ultrasonic extraction to determine polycyclic aromatic hydrocarbons in needles and bark of Pinus pinaster Ait. and Pinus pinea L. by GC-MS.

    Science.gov (United States)

    Ratola, Nuno; Lacorte, Sílvia; Barceló, Damià; Alves, Arminda

    2009-01-15

    Two different extraction strategies (microwave-assisted extraction (MAE) and ultrasonic extraction (USE)) were tested in the extraction of the 16 US Environmental Protection Agency (EPA) polycyclic aromatic hydrocarbons (PAHs) from pine trees. Extraction of needles and bark from two pine species common in the Iberian Peninsula (Pinus pinaster Ait. and Pinus pinea L.) was optimized using two amounts of sample (1g and 5 g) and two PAHs spiking levels (20 ng/g and 100 ng/g). In all cases, the clean-up procedure following extraction consisted in solid-phase extraction (SPE) with alumina cartridges. Quantification was done by gas chromatography (GC) with mass spectrometry (MS), using five deuterated PAH surrogate standards as internal standards. Limits of detection were globally below 0.2 ng/g. The method was robust for the matrices studied regardless of the extraction procedures. Recovery values between 70 and 130% were reached in most cases, except for high molecular weight PAHs (indeno[1,2,3-cd]pyrene, dibenzo[a,h]anthracene and benzo[ghi]perylene). A field study with naturally contaminated samples from eight sites (four in Portugal and four in Catalonia, Spain) showed that needles are more suitable biomonitors for PAHs, yielding concentrations from 2 to 17 times higher than those found in bark. The levels varied according to the sampling site, with the sum of the individual PAH concentrations between 213 and 1773 ng/g (dry weight). Phenanthrene was the most abundant PAH, followed by fluoranthene, naphthalene and pyrene.

  8. Salicylic acid up-regulates the expression of chloroplastic Cu, Zn-superoxide dismutase in needles of maritime pine (Pinus pinaster Ait.)

    OpenAIRE

    Azevedo, Herlânder; Lino-Neto, Teresa; Tavares, Rui

    2004-01-01

    International audience; Several studies have supported a major role of salicylic acid in modulating plant response against abiotic and biotic stresses, by induction antioxidant capacity. In this work, a full-length cDNA encoding a Cu,Zn - superoxide dismutase was isolated by screening a Pinus pinaster needle cDNA library. The predicted protein of 215 amino acid residues has a molecular mass of 22.1 kDa and exhibits a N-terminal transit peptide, which putatively targets the protein to the chlo...

  9. Effects of Recent Minimum Temperature and Water Deficit Increases on Pinus pinaster Radial Growth and Wood Density in Southern Portugal

    Science.gov (United States)

    Kurz-Besson, Cathy B.; Lousada, José L.; Gaspar, Maria J.; Correia, Isabel E.; David, Teresa S.; Soares, Pedro M. M.; Cardoso, Rita M.; Russo, Ana; Varino, Filipa; Mériaux, Catherine; Trigo, Ricardo M.; Gouveia, Célia M.

    2016-01-01

    Western Iberia has recently shown increasing frequency of drought conditions coupled with heatwave events, leading to exacerbated limiting climatic conditions for plant growth. It is not clear to what extent wood growth and density of agroforestry species have suffered from such changes or recent extreme climate events. To address this question, tree-ring width and density chronologies were built for a Pinus pinaster stand in southern Portugal and correlated with climate variables, including the minimum, mean and maximum temperatures and the number of cold days. Monthly and maximum daily precipitations were also analyzed as well as dry spells. The drought effect was assessed using the standardized precipitation-evapotranspiration (SPEI) multi-scalar drought index, between 1 to 24-months. The climate-growth/density relationships were evaluated for the period 1958-2011. We show that both wood radial growth and density highly benefit from the strong decay of cold days and the increase of minimum temperature. Yet the benefits are hindered by long-term water deficit, which results in different levels of impact on wood radial growth and density. Despite of the intensification of long-term water deficit, tree-ring width appears to benefit from the minimum temperature increase, whereas the effects of long-term droughts significantly prevail on tree-ring density. Our results further highlight the dependency of the species on deep water sources after the juvenile stage. The impact of climate changes on long-term droughts and their repercussion on the shallow groundwater table and P. pinaster’s vulnerability are also discussed. This work provides relevant information for forest management in the semi-arid area of the Alentejo region of Portugal. It should ease the elaboration of mitigation strategies to assure P. pinaster’s production capacity and quality in response to more arid conditions in the near future in the region. PMID:27570527

  10. Adaptation of fine roots to annual fertilization and irrigation in a 13-year-old Pinus pinaster stand.

    Science.gov (United States)

    Bakker, M R; Jolicoeur, E; Trichet, P; Augusto, L; Plassard, C; Guinberteau, J; Loustau, D

    2009-02-01

    Effects of fertilization and irrigation on fine roots and fungal hyphae were studied in 13-year-old maritime pine (Pinus pinaster Aït. in Soland), 7 years after the initiation of the treatments. The fertilization trials consisted of a phosphorus treatment, a complete fertilizer treatment (N, P, K, Ca and Mg), and an unfertilized treatment (control). Fertilizers were applied annually and were adjusted according to foliar target values. Two irrigation regimes (no irrigation and irrigation of a set amount each day) were applied from May to October. Root samples to depths of 120 cm were collected in summer of 2005, and the biomass of small roots (diameter 2-20 mm) and fine roots (diameter fertilizer treatments reduced the size of the fine root system, especially in the top soil layers, but did not affect small roots. Compared with control treatments, fine root morphology was affected by both fertilizer treatments with the fine roots having increased specific root length/area, and irrigation tended to reinforce this finer morphology. The amount of hyphae in the mesh ingrowth bags was higher in the fertilization and irrigation treatments than in the controls, suggesting further extension of the root system (ectomycorrhizal infection) and thus of the uptake system. Irrigation had no significant effect on the size of the fine root system, but resulted in a shallower rooting system. Total root to shoot ratios were unaffected by the treatments, but fine root mass:needle mass and fine root area index:leaf area index ratios decreased with increasing nutrient supply. Overall, compared with the control fine roots, increased nutrient supply resulted in a lower fine root biomass but the dynamic fraction of the finest roots was greater. Irrigation had only limited effects on fine root size, distribution and morphology.

  11. The role of population origin and microenvironment in seedling emergence and early survival in Mediterranean maritime pine (Pinus pinaster Aiton.

    Directory of Open Access Journals (Sweden)

    Natalia Vizcaíno-Palomar

    Full Text Available Understanding tree recruitment is needed to forecast future forest distribution. Many studies have reported the relevant ecological factors that affect recruitment success in trees, but the potential for genetic-based differences in recruitment has often been neglected. In this study, we established a semi-natural reciprocal sowing experiment to test for local adaptation and microenvironment effects (evaluated here by canopy cover in the emergence and early survival of maritime pine (Pinus pinaster Aiton, an emblematic Mediterranean forest tree. A novel application of molecular markers was also developed to test for family selection and, thus, for potential genetic change over generations. Overall, we did not find evidence to support local adaptation at the recruitment stage in our semi-natural experiment. Moreover, only weak family selection (if any was found, suggesting that in stressful environments with low survival, stochastic processes and among-year climate variability may drive recruitment. Nevertheless, our study revealed that, at early stages of recruitment, microenvironments may favor the population with the best adapted life strategy, irrespectively of its (local or non-local origin. We also found that emergence time is a key factor for seedling survival in stressful Mediterranean environments. Our study highlights the complexity of the factors influencing the early stages of establishment of maritime pine and provides insights into possible management actions aimed at environmental change impact mitigation. In particular, we found that the high stochasticity of the recruitment process in stressful environments and the differences in population-specific adaptive strategies may difficult assisted migration schemes.

  12. Descendant root volume varies as a function of root type: estimation of root biomass lost during uprooting in Pinus pinaster

    Directory of Open Access Journals (Sweden)

    Frédéric eDanjon

    2013-10-01

    Full Text Available Root systems of woody plants generally display a strong relationship between the cross-sectional area (CSA or cross-sectional diameter (CSD of a root and the dry weight of biomass (DWd or root volume (Vd that has grown (i.e., is descendent from that point. Specification of this relationship allows one to quantify root architectural patterns and estimate the amount of material lost when root systems are extracted from the soil. However, specifications of this relationship generally do not account for the fact that root systems are comprised of multiple types of roots. We assessed whether the relationship between CSD and Vd varies as a function of root type. Additionally, we sought to identify a more accurate and time-efficient method for estimating missing root volume than is currently available.We used a database that described the 3D root architecture of Pinus pinaster root systems (5, 12, or 19 yr from a stand. We determined the relationship between CSD and Vd for 10,000 root segments from intact root branches. Models were specified that did and did not account for root type. The relationships were then applied to the diameters of 11,000 broken root ends to estimate the volume of missing roots.CSD was nearly linearly related to the square root of Vd, but the slope of the curve varied greatly as a function of root type. Sinkers and deep roots tapered rapidly, as they were limited by available soil depth. Distal shallow roots tapered gradually, as they were less limited spatially. We estimated that younger trees lost an average of 17% of root volume when excavated, while older trees lost 4%. Missing volumes were smallest in the central parts of root systems and largest in distal shallow roots.The slopes of the curves for each root type are synthetic parameters that account for differentiation due to genetics, soil properties, or mechanical stimuli. Accounting for this differentiation is critical to estimating root loss accurately.

  13. Change of the temporal and spatial distribution of precipitation by a tree cover of Pinus pinaser; Modificacion de la distribucion temporal y espacial de la precipitacion por una cubierta arborea de Pinus pinaster

    Energy Technology Data Exchange (ETDEWEB)

    Rodriguez Suarez, J. A.; Diaz-Fierros, F.; Soto, B.

    2009-07-01

    Throughfall and stem flow volume generation was measured over 1 year period in a Pinus pinaster stand 9 years old. Throughfall was measured using 8 collectors in a fixed position connected to a tipping bucket rainfall gauge in a representative 10 x 10 m plot of the forest and stem flow was measured in three trees using a rubber ring around the trunk connected to a tipping bucket rainfall gauge. The two tipping bucket rainfall gauges was connected to a data logger programmed to record data every 5 minutes. (Author) 4 refs.

  14. Potencialidades do Pinus pinaster e Cytisus multiflorus na fitoestabilização de escombreiras na mina de ouro de Santo António (Penedono Potencialities of Pinus pinaster and Cytisus multiflorus on the phytostabilization of the Santo António (Penedono gold mine dumps

    Directory of Open Access Journals (Sweden)

    M.M. Abreu

    2007-07-01

    Full Text Available A mina de Santo António, localizada a cerca de 10 km ao norte de Penedono (Viseu, foi um importante centro mineiro, tendo-se aí principalmente explorado o ouro e outros elementos químicos, como o arsénio. Actualmente, todo o complexo mineiro está abandonado e bastante degradado. As escombreiras, cortadas por dois barrancos de grande dimensão, estão sujeitas a intensa erosão eólica e hídrica. Estas apresentam teores totais elevados para vários elementos, dos quais se destacam As, Pb, Cd e Au que atingem, respectivamente, 40 000, 653, 14 e 9,8 mg kg-1 . Estudou-se na área mineira, e em particular na envolvente das escombreiras, os solos e as plantas aí desenvolvidas (Pinus pinaster, Quercus pyrenaica, Castanea sativa, Cytisus multiflorus e uma gramínea do género Agrostis. As águas de drenagem da escombreira não vegetalizada apresentavam valores de pH de 2,58 e teores de As e Cd respectivamente de 160 e 0,05 mg/L. As águas recolhidas a jusante de escombreiras vegetalizadas com pinheiros e giestas, mostraram um decréscimo drástico de todos os elementos, em especial do As que ocorria com valores ambientalmente aceitáveis (The Santo António mine is located 10 km north of Penedono (Viseu, Portugal. This mine was in the first half of the twenty’s century an important gold and arsenic mining centre. Nowadays the mining complex is abandoned and presents high level of physical degradation. The waste dumps crossed by two large gullies are under strong water and wind erosion. The total concentrations of As, Pb, Cd e Au, in the dump material reached values as high as 40 000, 653, 14 and 9,8 mg kg-1, respectively. Soils and native plants as Pinus pinaster, Quercus pyrenaica, Castanea sativa, Cytisus multiflorus and gramineous plants (gen. Agrostis from the mining area and the waste dumps surroundings were collect and analysed. The draining waters from a no vegetated dump presented a pH value of 2.58, and As and Cd concentrations of 160

  15. FlorNExT®, a cloud computing application to estimate growth and yield of maritime pine (Pinus pinaster Ait.) stands in Northeastern Portugal

    Energy Technology Data Exchange (ETDEWEB)

    Barreiro, S.; Rua, J.; Tomé, M.

    2016-07-01

    Aim of the study. To introduce and describe FlorNExT®, a free cloud computing application to estimate growth and yield of maritime pine (Pinus pinaster Ait.) even-aged stands in the Northeast of Portugal (NE Portugal). Area of study: NE Portugal. Material and methods: FlorNExT® implements a dynamic growth and yield modelling framework which integrates transition functions for dominant height (site index curves) and basal area, as well as output functions for tree and stand volume, biomass, and carbon content. Main results: FlorNExT® is freely available from any device with an Internet connection at: http://flornext.esa.ipb.pt/. Research highlights: This application has been designed to make it possible for any stakeholder to easily estimate standing volume, biomass, and carbon content in maritime pine stands from stand data, as well as to estimate growth and yield based on four stand variables: age, density, dominant height, and basal area. FlorNExT® allows planning thinning treatments. FlorNExT® is a fundamental tool to support forest mobilization at local and regional scales in NE Portugal. (Author)

  16. FlorNExT®, a cloud computing application to estimate growth and yield of maritime pine (Pinus pinaster Ait. stands in Northeastern Portugal

    Directory of Open Access Journals (Sweden)

    Fernando Pérez-Rodríguez

    2016-07-01

    Full Text Available Aim of the study: To introduce and describe FlorNExT®, a free cloud computing application to estimate growth and yield of maritime pine (Pinus pinaster Ait. even-aged stands in the Northeast of Portugal (NE Portugal. Area of study: NE Portugal. Material and methods: FlorNExT® implements a dynamic growth and yield modelling framework which integrates transition functions for dominant height (site index curves and basal area, as well as output functions for tree and stand volume, biomass, and carbon content. Main results: FlorNExT® is freely available from any device with an Internet connection at: http://flornext.esa.ipb.pt/. Research highlights: This application has been designed to make it possible for any stakeholder to easily estimate standing volume, biomass, and carbon content in maritime pine stands from stand data, as well as to estimate growth and yield based on four stand variables: age, density, dominant height, and basal area. FlorNExT® allows planning thinning treatments. FlorNExT® is a fundamental tool to support forest mobilization at local and regional scales in NE Portugal. Keywords: forest management; maritime pine; forest modelling; knowledge transfer tool.

  17. Potassium nutrition of ectomycorrhizal Pinus pinaster: overexpression of the Hebeloma cylindrosporum HcTrk1 transporter affects the translocation of both K(+) and phosphorus in the host plant.

    Science.gov (United States)

    Garcia, Kevin; Delteil, Amandine; Conéjéro, Geneviève; Becquer, Adeline; Plassard, Claude; Sentenac, Hervé; Zimmermann, Sabine

    2014-02-01

    Mycorrhizal associations are known to improve the hydro-mineral nutrition of their host plants. However, the importance of mycorrhizal symbiosis for plant potassium nutrition has so far been poorly studied. We therefore investigated the impact of the ectomycorrhizal fungus Hebeloma cylindrosporum on the potassium nutrition of Pinus pinaster and examined the involvement of the fungal potassium transporter HcTrk1. HcTrk1 transcripts and proteins were localized in ectomycorrhizas using in situ hybridization and EGFP translational fusion constructs. Importantly, an overexpression strategy was performed on a H. cylindrosporum endogenous gene in order to dissect the role of this transporter. The potassium nutrition of mycorrhizal pine plants was significantly improved under potassium-limiting conditions. Fungal strains overexpressing HcTrk1 reduced the translocation of potassium and phosphorus from the roots to the shoots of inoculated plants in mycorrhizal experiments. Furthermore, expression of HcTrk1 and the phosphate transporter HcPT1.1 were reciprocally linked to the external inorganic phosphate and potassium availability. The development of these approaches provides a deeper insight into the role of ectomycorrhizal symbiosis on host plant K(+) nutrition and in particular, the K(+) transporter HcTrk1. The work augments our knowledge of the link between potassium and phosphorus nutrition via the mycorrhizal pathway.

  18. Carbon and nitrogen accumulation in forest floor and surface soil under different geographic origins of Maritime pine (Pinus pinaster Aiton.) plantations

    Energy Technology Data Exchange (ETDEWEB)

    Ozdemir, E.; Oral, H. V.; Akburak, S.; Makineci, E.; Yilmaz, E.

    2013-09-01

    Aim of study: To determine if plantations consisting of different geographic origins of the Maritime pine (Pinus pinaster Aiton.) could have altered C and N stocks in the forest floor and surface soils. Area of study: Forest floor and mineral soil C and N stocks were measured in four adjacent plantations of different geographic origins of Maritime pine (Gironde, Toulon, Corsica and Spain) and adjacent primary native Sessile oak (Quercus petraea L.) at Burunsuz region in Belgrad Forest where is located in the Istanbul province in the Marmara geographical region between 41° 09’-41° 12’ N latitude and 28° 54’-29° 00’ E longitude in Turkey. Material and methods: Plots were compared as common garden experiments without replications. 15 surface soil (0-10 cm) and 15 forest floor samples were taken from each Maritime pine origins and adjacent native Sessile oak forest. C and N contents were determined on LECO Truspec 2000 CN analyzer. The statistical significance of the results was evaluated by one-way Analysis of Variance (ANOVA). Research highlights: Forest floor carbon mass, nitrogen concentration and nitrogen mass of forest floor showed a significant difference among origins. Soil carbon mass and nitrogen mass did not significantly differ among investigated plots. (Author)

  19. Phosphorus acquisition from phytate depends on efficient bacterial grazing, irrespective of the mycorrhizal status of Pinus pinaster

    OpenAIRE

    2012-01-01

    Background and aims : Phosphorus from phytate, although constituting the main proportion of organic soil P, is unavailable to plants. Despite the well-known effects of rhizosphere trophic relationships on N mineralization, no work has been done yet on P mineralization. We hypothesized that the interactions between phytate-mineralizing bacteria, mycorrhizal fungi and bacterial grazer nematodes are able to improve plant P use from phytate. Methods : We tested this hypothesis by growing Pinus pi...

  20. Estudio de la variabilidad genética de Pinus Pinaster ait. en un ensayo de procedencias situado en el término municipal de La Cistérniga (Valladolid)

    OpenAIRE

    Manso Escribano, Sara

    2015-01-01

    En este TFM se estudia el crecimiento y la rectitud del tallo de Pinus pinaster Ait. de 6 años de edad en una parcela de ensayo de procedencias situada en el término municipal de La Cistérniga, provincia de Valladolid, y se encuadra en un proyecto más amplio de seguimiento de dicha parcela. Estos ensayos son importantes para precisar el crecimiento y adaptación de las distintas procedencias, así como para poder efectuar recomendaciones de uso de Materiales Forestales de Reproducción de las di...

  1. Biophysical modelling of intra-ring variations in tracheid features and wood density of Pinus pinaster trees exposed to seasonal droughts.

    Science.gov (United States)

    Wilkinson, Sarah; Ogée, Jérôme; Domec, Jean-Christophe; Rayment, Mark; Wingate, Lisa

    2015-03-01

    Process-based models that link seasonally varying environmental signals to morphological features within tree rings are essential tools to predict tree growth response and commercially important wood quality traits under future climate scenarios. This study evaluated model portrayal of radial growth and wood anatomy observations within a mature maritime pine (Pinus pinaster (L.) Aït.) stand exposed to seasonal droughts. Intra-annual variations in tracheid anatomy and wood density were identified through image analysis and X-ray densitometry on stem cores covering the growth period 1999-2010. A cambial growth model was integrated with modelled plant water status and sugar availability from the soil-plant-atmosphere transfer model MuSICA to generate estimates of cell number, cell volume, cell mass and wood density on a weekly time step. The model successfully predicted inter-annual variations in cell number, ring width and maximum wood density. The model was also able to predict the occurrence of special anatomical features such as intra-annual density fluctuations (IADFs) in growth rings. Since cell wall thickness remained surprisingly constant within and between growth rings, variations in wood density were primarily the result of variations in lumen diameter, both in the model and anatomical data. In the model, changes in plant water status were identified as the main driver of the IADFs through a direct effect on cell volume. The anatomy data also revealed that a trade-off existed between hydraulic safety and hydraulic efficiency. Although a simplified description of cambial physiology is presented, this integrated modelling approach shows potential value for identifying universal patterns of tree-ring growth and anatomical features over a broad climatic gradient.

  2. Determination of the Content and Structure of Polyprenols from Greek Pinus pinaster Leaves%希腊海岸松叶中聚戊烯醇含量与化学结构分析

    Institute of Scientific and Technical Information of China (English)

    段鑫; 陶冉; 王成章; 薛志革

    2014-01-01

    The isopentenyl unit numbers of polyprenols from Greek Pinus pinaster leaves was 14 to 20 by contrasting the retention time of polyprenol reference substance using HPLC. And the polyprenols structure was confirmed asω-trans2-cisn-OH by 1 H and 13C spectrum. The HPLC conditions of a Kromasil C18 ODS-1(150 mm × 4. 6 mm, 5 μm) column, methanol-isopropanol (9∶16,v/v) as the mobile phase at a flow rate of 0. 50 mL/min, column temperature of 25 ℃,PDA detector with detective wavelength of 210 nm were used. The polyprenols from Greek P. pinaster leaves in the range of 1. 10 -12. 10 μg had good linearity (r=0.996 3), the average recovery was 98. 21 %, and RSD was 2. 27 % (n =6). The result showed that the polyprenols content of dried leaves was 0. 33 % . The method was accurate, reproducible and quick to determine the isopentenyl unit number and content of polyprenols from Greek P. pinaster leaves.%利用HPLC与聚戊烯醇对照品保留时间对照的定性方法,确定海岸松叶中聚戊烯醇的异戊烯基单元数为14~20;利用1H NMR和13C NMR确定海岸松聚戊烯醇化学结构为ω-trans2-cisn-OH构型。采用Kromasil C18 ODS-1(150 mm ×4.6 mm,5μm)色谱柱,以甲醇-异丙醇(体积比9∶16)为流动相,流速为0.50 mL/min,检测波长为210 nm,柱温为25℃,PDA检测器等色谱条件,测定海岸松叶聚戊烯醇在1.10~12.10μg范围内线性良好(r=0.9963),平均回收率为98.21%,RSD为2.27%(n=6),海岸松干叶中聚戊烯醇含量为0.33%。

  3. The host plant Pinus pinaster exerts specific effects on phosphate efflux and polyphosphate metabolism of the ectomycorrhizal fungus Hebeloma cylindrosporum: a radiotracer, cytological staining and (31) P NMR spectroscopy study.

    Science.gov (United States)

    Torres-Aquino, Margarita; Becquer, Adeline; Le Guernevé, Christine; Louche, Julien; Amenc, Laurie K; Staunton, Siobhan; Quiquampoix, Hervé; Plassard, Claude

    2017-02-01

    Ectomycorrhizal (ECM) association can improve plant phosphorus (P) nutrition. Polyphosphates (polyP) synthesized in distant fungal cells after P uptake may contribute to P supply from the fungus to the host plant if they are hydrolyzed to phosphate in ECM roots then transferred to the host plant when required. In this study, we addressed this hypothesis for the ECM fungus Hebeloma cylindrosporum grown in vitro and incubated without plant or with host (Pinus pinaster) and non-host (Zea mays) plants, using an experimental system simulating the symbiotic interface. We used (32) P labelling to quantify P accumulation and P efflux and in vivo and in vitro nuclear magnetic resonance (NMR) spectroscopy and cytological staining to follow the fate of fungal polyP. Phosphate supply triggered a massive P accumulation as newly synthesized long-chain polyP in H. cylindrosporum if previously grown under P-deficient conditions. P efflux from H. cylindrosporum towards the roots was stimulated by both host and non-host plants. However, the host plant enhanced (32) P release compared with the non-host plant and specifically increased the proportion of short-chain polyP in the interacting mycelia. These results support the existence of specific host plant effects on fungal P metabolism able to provide P in the apoplast of ectomycorrhizal roots.

  4. The effects of the recent minimum temperature and water deficit increases on Pinus pinaster wood radial growth and density in southern Portugal.

    Directory of Open Access Journals (Sweden)

    Cathy Béatrice Kurz Besson

    2016-08-01

    Full Text Available Western Iberia has recently shown increasing frequency of drought conditions coupled with heatwave events, leading to exacerbated limiting climatic conditions for plant growth. It is not clear to what extent wood growth and density of agroforestry species have suffered from such changes or recent extreme climate events.To address this question, tree-ring width and density chronologies were built for a P. pinaster stand in southern Portugal and correlated with climate variables, including the minimum, mean and maximum temperatures and the number of cold days. Monthly and maximum daily precipitations were also analyzed as well as dry spells. The drought effect was assessed using the standardized precipitation-evapotranspiration (SPEI multi-scalar drought index, between 1 to 24-months. The climate-growth/density relationships were evaluated for the period 1958-2011.We show that both wood radial growth and density highly benefit from the strong decay of cold days and the increase of minimum temperature. Yet the benefits are hindered by long-term water deficit, which results in different levels of impact on wood radial growth and density. Despite of the intensification of long-term water deficit, tree-ring width appears to benefit from the minimum temperature increase, whereas the effects of long-term droughts significantly prevail on tree-ring density. Our results further highlight the dependency of the species on deep water sources after the juvenile stage. The impact of climate changes on long-term droughts and their repercussion on the shallow groundwater table and P. pinaster’s vulnerability are also discussed. This work provides relevant information for forest management in the semi-arid area of the Alentejo region of Portugal. It should ease the elaboration of mitigation strategies to assure P. pinaster’s production capacity and quality in response to more arid conditions in the near future in the region.

  5. 感染松萎蔫病的葡萄牙海岸松中内生细菌的分离%Endophytic bacteria isolated from pine wilt-affected Pinus pinaster trees

    Institute of Scientific and Technical Information of China (English)

    Vieira dos Santos M C; Proenca D A N; Fonseca L; Abrantes I M de O; Morais P V

    2011-01-01

    The characteristic rapid death of maritime pine, Pinus pinaster, after infection by the pinewood nematode (PWN), Bursaphelenchus xylophilus, suggests the involvement of other organisms in pine wilt disease (PWD) . Endophytic bacteria are ubiquitous in nature and colonize a broad spectrum of plant species. They colonize plants internally without harming them and although they have been reported to promote plant growth and stimulate plant defence mechanisms. Although such bacteria may be involved in improving plant health, they have also been seen as a potential cause of the low regeneration capacity of mature trees. The bacteria carried out by the PWN throughout pine trees may also contribute to the rapid wilting of the maritime pine trees. This study was made to assess the endophytic microbial community structure of pine wilt affected and non-affected P. pinaster, based on culture isolates, amplified ribosomal DNA restriction analysis (ARDRA) and denaturing gradient gel electrophorosis (DGGE) profiling, and to relate the results to a possible association between the characteristics and bacteria associated with the PWN. The microbial community from trees and PWNs included strains from Beta and Gammaproteobacteria, but Alphaproteobacteria and Bacteroidetes were only found in the endophytic community whereas Actinobacteria and Firmicutes were only found to be associated with the nematode. ARDRA and DGGE profiles showed differences in the diversity of the endophytic microbial community that may be related to the presence of the PWN. Those differences seem to be associated with the number of strains of the genera Burkholderia, Pseudomonas and Luteibacter. Isolates belonging to the species Janthinobacterium agaricidamnosum, P. lutea and Dyella yeojuensis were isolated from trees and were associated with the PWN. This research documents the presence of bacteria associated with the PWN by molecular methods and the impact of the nematode in the plant endophytic microbial

  6. Phytochemical analysis of Pinus eldarica bark.

    Science.gov (United States)

    Iravani, S; Zolfaghari, B

    2014-01-01

    Bark extract of Pinus pinaster contains numerous phenolic compounds such as catechins, taxifolin, and phenolic acids. These compounds have received considerable attentions because of their anti-inflammatory, antimutagenic, anticarcinogenic, antimetastatic and high antioxidant activities. Although P. pinaster bark has been intensely investigated in the past; there is comparably less information available in the literature in regard to P. eldarica bark. Therefore, the aim of this study was to determine the chemical composition of P. eldarica commonly found in Iran. A reversed-phase high pressure liquid chromatography (RP-HPLC) method for the determination of catechin, caffeic acid, ferulic acid, and taxifolin in P. pinaster and P. eldarica was developed. A mixture of 0.1% formic acid in deionized water and 0.1% formic acid in acetonitrile was used as the mobile phase, and chromatographic separation was achieved on a Nova pack C18 at 280 nm. The two studied Pinus species contained high amounts of polyphenolic compounds. Among four marker compounds, the main substances identified in P. pinaster and P. eldarica were taxifolin and catechin, respectively. Furthermore, the composition of the bark oil of P. eldarica obtained by hydrodistillation was analyzed by gas chromatography/mass spectroscopy (GC/MS). Thirty-three compounds accounting for 95.1 % of the oil were identified. The oils consisted mainly of mono- and sesquiterpenoid fractions, especially α-pinene (24.6%), caryophyllene oxide (14.0%), δ-3-carene (10.7%), (E)-β-caryophyllene (7.9%), and myrtenal (3.1%).

  7. Sergio Bravo y tendencias del montaje Film EditingTendencies in Sergio Bravo's Filmography

    OpenAIRE

    Pablo Corro Pemjean

    2010-01-01

    El texto ahonda, en términos, críticos tanto en la singular doctrina sobre el montaje cinematográfico propuesta por Gilles Deleuze en sus textos sobre cine, como en el sentido y forma que adopta el montaje en la filmografía del documentalista chileno Sergio Bravo Ramos. Las escuelas de montaje, que distingue el filósofo y que, como escuelas cinematográficas nacionales son asimiladas a conciencias estético-políticas, se identifican en los documentales de Bravo y permiten distinguir, a través d...

  8. Manuel Alvarez Bravo, photographe mexicain de l’abstraction figurative

    OpenAIRE

    Laurent Aubague

    2011-01-01

    Parmi les multiples facettes de la longue œuvre de Manuel Alvarez Bravo (1902-2002), celle qui correspond à la décennie des années 1925-1935 peut susciter un intérêt particulier dans la mesure où elle semble répondre à une orientation esthétique originale, apparemment contradictoire, pouvant être qualifiée d’abstraction figurative.When considering the multi-faceted work Manuel Alvarez Bravo produced throughout his lifetime (1902-2002), the decade 1925-35 offers particular interest insofar as ...

  9. CASTLE BRAVO: Fifty Years of Legend and Lore. A Guide to Off-Site Radiation Exposures

    Science.gov (United States)

    2013-01-31

    old sailing sloop Seeadler. January 2013 CASTLE BRAVO: Fifty Years of Legend and Lore Introduction 9 Article 22 of the League of Nations...DTRIAC SR-12-001 CASTLE BRAVO: FIFTY YEARS OF LEGEND AND LORE A Guide to Off-Site Radiation Exposures January 2013 Distribution A: Approved for...2013 2. REPORT TYPE Special Report 3. DATES COVERED (From-To) 1954 4. TITLE AND SUBTITLE Castle Bravo: Fifty Years of Legend and Lore A Guide to

  10. Alternativas de mejora de Pinus pinaster Ait. para el interior de Galicia.

    OpenAIRE

    De La Mata Pombo, R.; Zas Arregui, Rafael; Merlo Sánchez, E.; Sampedro Pérez, Luis

    2009-01-01

    14 páginas, 3 tablas, 3 figuras / 12 diapositivas -- Comunicación presentada al 5º Congreso Forestal Español que se celebró del 21 y 25 de septiembre de 2009 en Ávila, bajo el lema “Montes y sociedad: saber qué hacer”.

  11. El programa de mejora genética de Pinus pinaster en Galicia

    OpenAIRE

    Zas, R; Merlo, E.

    2008-01-01

    El pino marítimo es una de las especies más importantes del sector forestal gallego. Fruto de esta importancia, desde los años 80 se lleva a cabo un programa de mejora cuyo fin último es el abastecimiento de semilla de alta calidad genética pasa su uso en repoblación. En este trabajo se revisan las actuaciones realizadas al respecto tanto en la zona costera como en el interior de Galicia. Se describe el dispositivo experimental del programa, los objetivos y la estra...

  12. Impact of wildfire on the edaphic microarthropod community in a Pinus pinaster forest in central Italy

    Directory of Open Access Journals (Sweden)

    Lisa C

    2015-12-01

    Full Text Available The purpose of this study was to investigate the response of the soil microarthropod community to wildfire in forest ecosystems. The edaphic microarthropod communities of pine stands burned in 2001, in 2009, both in 2001 and in 2009, in 2012 were compared to an area never burned. Sampling was conducted in the spring and autumn of 2011 and 2012 in the areas burned in 2001, in 2009 and those never burned, while in the area burned in 2012, soil samples were collected in March (10 days after fire, June and September. The abundance and biodiversity of the microarthropod community were assessed. A multitemporal analysis was also carried out to assess the effect of fire on soil microarthropod abundance 5 months, 2-3 years and 10-11 years after fire. The results showed that the abundance of edaphic microarthropod communities decreased dramatically in areas burned twice, but also in areas burned once in 2009 and five months after the fire in the area burned in 2012. Different taxonomic groups did not seem to respond to fire in the same way, some taxa being more sensitive than others. Pseudoscorpionida decreased in both the short- and the long-term, while Diplopoda, Thysanoptera and Symphyla showed a reduction in the intermediate- and long-term. In the short-term, Diptera and Coleoptera larvae appeared to be the most influenced taxonomic groups. Our study shows that biomonitoring is a valuable tool to investigate the reaction of forest ecosystems to fire, and that edaphic microarthropods can provide interesting answers about the direct and indirect effects of fire on soil.

  13. Mechanical properties of structural maritime pine sawn timber from Galicia (Pinus pinaster Ait. ssp. atlantica)

    Energy Technology Data Exchange (ETDEWEB)

    Carballo, J.; Hermoso, E.; Fernandez-Golfin, J. I.

    2009-07-01

    The use of maritime pine sawn timber in structural applications requires knowledge of its mechanical properties. Standards have changed, however, since the last research on this timber was performed. In the present study, 491 beams of maritime pine from Gaelic, of structural-use size but different cross-section, were tested according to these modified standards. Each beam was visually graded according to standard UNE 56.544 and subjected to a four point bending test. The strength classes assigned by the visual grades awarded suggest this material to have greater structural capacity than that currently assumed. The relationships between the modulus of elasticity, strength and density were also examined. (Author) 25 refs.

  14. High-density SNP assay development for genetic analysis in maritime pine (Pinus pinaster).

    Science.gov (United States)

    Plomion, C; Bartholomé, J; Lesur, I; Boury, C; Rodríguez-Quilón, I; Lagraulet, H; Ehrenmann, F; Bouffier, L; Gion, J M; Grivet, D; de Miguel, M; de María, N; Cervera, M T; Bagnoli, F; Isik, F; Vendramin, G G; González-Martínez, S C

    2016-03-01

    Maritime pine provides essential ecosystem services in the south-western Mediterranean basin, where it covers around 4 million ha. Its scattered distribution over a range of environmental conditions makes it an ideal forest tree species for studies of local adaptation and evolutionary responses to climatic change. Highly multiplexed single nucleotide polymorphism (SNP) genotyping arrays are increasingly used to study genetic variation in living organisms and for practical applications in plant and animal breeding and genetic resource conservation. We developed a 9k Illumina Infinium SNP array and genotyped maritime pine trees from (i) a three-generation inbred (F2) pedigree, (ii) the French breeding population and (iii) natural populations from Portugal and the French Atlantic coast. A large proportion of the exploitable SNPs (2052/8410, i.e. 24.4%) segregated in the mapping population and could be mapped, providing the densest ever gene-based linkage map for this species. Based on 5016 SNPs, natural and breeding populations from the French gene pool exhibited similar level of genetic diversity. Population genetics and structure analyses based on 3981 SNP markers common to the Portuguese and French gene pools revealed high levels of differentiation, leading to the identification of a set of highly differentiated SNPs that could be used for seed provenance certification. Finally, we discuss how the validated SNPs could facilitate the identification of ecologically and economically relevant genes in this species, improving our understanding of the demography and selective forces shaping its natural genetic diversity, and providing support for new breeding strategies.

  15. Cartas de un general porfirista: Correspondencia familiar de Ignacio Bravo, 1889-1918 Letters from a Porfirian General: Family Correspondence of Ignacio Bravo, 1889-1918

    Directory of Open Access Journals (Sweden)

    Martín Ramos Díaz

    2012-08-01

    Full Text Available La correspondencia familiar de Ignacio Bravo muestra facetas poco conocidas de los primeros años de vida del territorio de Quintana Roo, de algunos episodios de la revolución mexicana y del exilio del presidente Victoriano Huerta en Texas. Pero, sobre todo, las cartas personales del general Bravo delinean el itinerario de una familia de militares en los años finales del siglo XIX y primeros del siglo XX, en el México de Porfirio Díaz. El presente artículo da la noticia del hallazgo de cientos de piezas de correspondencia personal de Bravo y al mismo tiempo realiza una antología de la información que ayuda a comprender, con nuevos datos, sucesos recurrentes en la historiografía regional del sureste mexicano, como la pacificación de los mayas, la creación del territorio federal de Quintana Roo en la frontera México-Belice y el entorno selvático y hostil para los pioneros que se establecieron en esa alejada región.Ignacio Bravo's family correspondence reveals little-known aspects of the early years of Quintana Roo, certain episodes of the Mexican Revolution and President Victoriano Huerta's exile in Texas. Above all, General Bravo's personal letters trace the lives of a family of military men in the late 19th and early 20th century in the Mexico of Porfirio Díaz. The article reveals the discovery of hundreds of pieces of Bravo's personal correspondence and provides an anthology of the information that uses new data to explain recurrent events in the regional historiography of the Mexican southeast, such as the pacification of the Maya, the creation of the federal territory of Quintana Roo on the Mexico-Belize border and the hostile, jungle environment for the pioneers who settled in this far-off region.

  16. Economic Value of Water in the Rio Bravo Basin (Mexico) : The Case of Bajo San Juan, Juarez and Saltillo

    OpenAIRE

    2005-01-01

    This paper evaluates the economic value of water in three locations in the Rio Bravo Basin. Formally known as the Bravo-Conchos, this basin overlaps across five Mexican states, beneath the Rio Bravo in northeastern Mexico. The paper also estimates the level of economic output in terms of value added for the main economic activities, using official statistics. The evaluation of water use pr...

  17. Technical problems produced by the Bravo pH test in nonerosive reflux disease patients

    Institute of Scientific and Technical Information of China (English)

    Andrés; de; Hoyos; Edgar; Alain; Esparza

    2010-01-01

    AIM: To evaluate the technical failures of the Bravo pH test in a population with nonerosive gastroesophageal reflux disease. METHODS: Over the course of a year, we prospectively studied a population of 66 nonerosive reflux disease patients who received a Bravo pH test. The number and frequency of all technical failures were documented, quantified and analyzed. RESULTS: A total of 66 patients, with a mean age of 41.7 years, were studied. Technical failures occurred in 15.15% of the sample. The most frequent...

  18. Mediation of seed provisioning in the transmission of environmental maternal effects in Maritime pine (Pinus pinaster Aiton).

    Science.gov (United States)

    Zas, R; Cendán, C; Sampedro, L

    2013-09-01

    Although maternal environmental effects are increasingly recognized as an important source of phenotypic variation with relevant impacts in evolutionary processes, their relevance in long-lived plants such as pine trees is largely unknown. Here, we used a powerful sample size and a strong quantitative genetic approach to analyse the sources of variation of early seedling performance and to identify seed mass (SM)-dependent and -independent maternal environmental effects in Maritime pine. We measured SM of 8924 individual seeds collected from 10 genotypes clonally replicated in two environments of contrasting quality (favourable and stressful), and we measured seedling growth rate and biomass allocation to roots and shoots. SM was extremely variable (up to 14-fold) and strongly determined by the maternal environment and the genotype of the mother tree. The favourable maternal environment led to larger cones, larger seeds and reduced SM variability. The maternal environment also determined the offspring phenotype, with seedlings coming from the favourable environment being 35% larger and with greater root/shoot ratio. Transgenerational plasticity appears, thus, to be a relevant source of phenotypic variation in the early performance of this pine species. Seed provisioning explained most of the effect of the maternal environment on seedling total biomass. Environmental maternal effects on seedling biomass allocation were, however, determined through SM-independent mechanisms, suggesting that other epigenetic regulation channels may be involved.

  19. Instructional Design of Entrepreneurship Courses: Interview Research of Wyoming BRAVO! Entrepreneurs

    Science.gov (United States)

    Kolb, Belinda J.

    2010-01-01

    This qualitative study investigated the opportunity recognition process of Wyoming BRAVO! Entrepreneur (WBE) Award winners or nominees, in order to better inform the learner analysis and organizational strategy components of instructional design, specifically with respect to entrepreneurship courses. This study may be of significance to post…

  20. Unreported complication of Bravo pH capsule dislodged intothe pyriform sinus

    Institute of Scientific and Technical Information of China (English)

    2015-01-01

    We report an unexpected, previously unreported complicationof Bravo pH capsule dislodgement. During BravopH testing of a 44-year-old man with gastroesophagealreflux disease, we were unable to endoscopicallyvisualize the capsule attached to the esophageal wallafter deployment. After multiple attempts to detect thecapsule, it was visualized in the left pyriform sinus. Asthere was significant risk for pulmonary dislodgement,ENT and pulmonary physicians were immediatelyconsulted to review options for safe removal. Ultimately,ENT successfully retrieved the capsule with a foreignbody removal forceps. The Bravo pH test is generallya well-tolerated diagnostic tool used to confirm thepresence of abnormal esophageal acid reflux. While fewcomplications have been reported, technical difficultiescan occur, including poor data reception, misplacement,and early dislodgement. Rarely, more serious complicationscan occur, ranging from esophageal wall traumato capsule aspiration. Gastroenterologists performingthis procedure should be aware of the low, but nontrivial,risk of complications.

  1. Composition of pectic polysaccharides in a Portuguese apple (Malus domestica Borkh. cv Bravo de Esmolfe)

    OpenAIRE

    2014-01-01

    Malus domestica Borkh. cv Bravo de Esmolfe is a typical Portuguese apple cultivar classified as Protected Designation and Origin (PDO). It is a traditional product produced under strict conditions and labelled with a specific law protected designation. This cultivar presents quite good sweetness and flavor. The monosaccharide composition of the pectic polysaccharides from this traditional apple is herein reported for the first time. Based on the molar ratios obtained from the sugar compositio...

  2. Existing opportunities to adapt the Rio Grande/Bravo Basin Water Resources Allocation Framework

    OpenAIRE

    2016-01-01

    The study of the Rio Grande/Bravo (RGB) Basin water allocation demonstrates how the United States (U.S.) and Mexico have consolidated a transboundary framework based on water sharing. However, the water supply no longer meets the ever-increasing demand for water or the expectations of different stakeholders. This paper explores opportunities for an enhanced management regime that will address past problems and better examine how to balance demands for a precious resource and environmental nee...

  3. A Crítica ao Cinema Brasileiro Atual: a retórica das revistas Veja e Bravo!

    Directory of Open Access Journals (Sweden)

    GOMES, Regina

    2010-07-01

    Full Text Available The article presents the results of a rhetorical-comparative study of film reviews about Brazilian movies, published in magazines Veja and Bravo!, between the years 1997 and 2004. Although different institutions, the magazines used similar argumentative resources to convince the reader with aesthetic judgments predominantly positive of recent Brazilian cinema. However, Bravo! presented persuasive rhetoric more stronger that Veja magazine. The dialogue between rhetoric and journalistic criticism demarcated the discussion relating to the methods of interpretation and meaning construction in film reviews.

  4. José Julián Bravo (1874-1927), a hitherto unknown conchologist from Peru

    NARCIS (Netherlands)

    Mogollón Avila, V.; Breure, A.S.H.

    2009-01-01

    The recent finding of a private shell collection from Peru, dating from the early 20th century, sheds some new light on the history of Neotropical malacology. The collection of J.J. Bravo is partly reconstructed and some data is given on his life and conchological activities. It shows that, in the e

  5. Ordenamiento territorial, turismo y ambiente en Valle de Bravo, México

    Directory of Open Access Journals (Sweden)

    Nancy Sierra López

    2011-01-01

    Full Text Available Detrás de ese paisaje idílico de agua, bosques y arquitectura, que la mercadotecnia ofrece y que la tradición evoca, se desarrollaron fuertes conflictos de intereses, necesidades sociales insatisfechas, así como la depredación ambiental constante. En Valle de Bravo pueden observarse paisajes con diferentes visiones y lógicas socioeconómicas, como resultado de un conjunto de políticas de carácter territorial formuladas por el Estado y por la iniciativa privada, con el objetivo de fomentar la actividad turística como eje del desarrollo capitalista. El paisaje creado en algunas zonas ofrece una imagen de prosperidad y confort, mientras que en otras es posible observar la segregación socioterritorial que esta actividad ha generado y que excluye a la mayoría de sus habitantes de los beneficios que el turismo puede traer.

  6. Apunten, disparen, flash: Elena Poniatowska, Víctor Casasola y Manuel Álvarez Bravo

    OpenAIRE

    Soltero Sánchez, Evangelina

    2008-01-01

    Teniendo en cuenta la importancia de la fotografía en México, y de la misma en la obra de Elena Poniatowska, el artículo analiza las relaciones entre fotografía y literatura a través de dos obras de la escritora: Manuel Álvarez Bravo. El artista, su obra, sus tiempos (1991) y Las soldaderas (1999). La elección de los textos viene motivada, de un lado, por reproducirse en ellos imágenes de los dos camarógrafos más importantes de la fotografía mexicana del siglo XX; de otro, por tener orígenes ...

  7. Medical status of Marshallese accidentally exposed to 1954 Bravo fallout radiation, January 1983-December 1984

    Energy Technology Data Exchange (ETDEWEB)

    Adams, W.H.; Engle, J.R.; Harper, J.A.; Heotis, P.M.; Scott, W.A.

    1986-01-01

    March 1, 1984, was the 30th anniversary of the Bravo thermonuclear test that resulted in the accidental exposure of the populations of Rongelap and Utirik atolls to radioactive fallout. The chronicling of the medical events resulting from that exposure is continued in this report, which covers the period from January 1983 through December 1984. An updated listing of all relevant publications from the Medical Department Brookhaven National Laboratory, is presented in the Reference Section. Thirty years of observation continue to show no detectable increase in mortality in the exposed population as a result of that exposure. The survival curves of the high-exposure Rongelap group, the low-exposure Utirik population, and an unexposed group of Rongelap people matched by age and sex to the exposed Rongelap group in 1957 continue to be similar. 89 refs., 2 figs., 6 tabs.

  8. BRAVO/TeleTrend: a comprehensive WWW-based neuromonitoring system for the neurosurgery ICU.

    Science.gov (United States)

    Nenov, V I; Buxey, F; Yamaguchi, Y

    1999-01-01

    This paper describes BRAVO/TeleTrend--a comprehensive client/server-based system for remote access, review and analyses of continuously acquired multiparametric physiological data from Intensive Care unit (ICU) patients. The system is designed as a distributed three tier model and implemented in Java (Sun Microsystems). TeleTrend is a data review package, which interfaces to existing physiological bedside monitors such as the BRAVO suite of products (Nicolet Biomedical, Madison, WI) and the vital signs monitors compatible with the Unity Network (Marquette Electronics, Milwaukee, WI). It does not transfer over the web the entire patient record, which can be hundreds of megabytes. Instead, it provides tools to view a compressed representation of the raw data in a trend display and to zoom into the raw data if needed. Thus, it eliminates the need for a high-bandwidth Internet connection and makes possible the use of a slower modem access to the vast amount of physiological data acquired per patient. In addition, TeleTrend features a rule-based module capable of generating clinical alerts, which is a potentially useful tool for neurointensivists and other critical care personnel. Finally, TeleTrend is intended as a multi-user, semi real-time telemedical application, which features built-in white-board and chat components. These components allow several physicians at different locations around the world to simultaneously view and brainstorm over critical chunks of continuously recorded raw and trend data. By allowing the end-user user to switch on-the-fly from monitoring patients in one ICU to those in another, and by integrating an HL7 interface TeleTrend steps over the boundaries of a single ICU. Thus, it can be provide a medical enterprise-wide solution to the remote access of an important component of the electronic patient medical record. Currently in house validation, verification and alpha testing of the system are underway.

  9. Datos para una biografía del jurista Pedro Murillo Velarde y Bravo

    Directory of Open Access Journals (Sweden)

    Luis Díaz de la Guardia y López

    2001-01-01

    Full Text Available En el presente artículo se pretende aportar una serie de coordenadas para fijar la vida y el pensamiento de Pedro Murillo Velarde y Bravo (Laujar 1696-Puerto de Santa María 1753. Hidalgo de familia de caballeros, sobrino de un Obispo de Pamplona, rompe en principio con el entorno familiar de búsqueda de ascenso social para dedicarse a la misión en Filipinas, llegando a ser procurador provincial de la Compañía en Filipinas ante la Corte de Madrid y Roma. Gran jurista, tiistoriador, geógrafo, poeta, político y orador. Por medio de documentos y obras del autor no sólo se desentraña la vida del jesuíta sino también su pensamiento frente a las Filipinas, la política o la religión.This article intends to contribute a series of coordinates to establish the life and thinking of Pedro Murillo Velarde y Bravo (Laujuar 1696-Puerto de Santa María 1753. Noble from a family of gentlemen and nephew of a Bishop of Pamplona, firstly breaks away from the family environment that seeks social promotion to dedicate himself to the mission in the Philippines, becoming the Society of Jesus'provincial representative in the Philippines before the Court of Madrid and Rome. Great jurist, historian, geographer, poet, politician and orator. Through the documents and works of the autor, not only is the life of the Jesuit unravelled but also his thinking on the Philippines politics and religión.

  10. Prácticas ambientales de las empresas turísticas en valle de Bravo

    Directory of Open Access Journals (Sweden)

    Hernández Peñaloza1 Nadia

    2012-05-01

    Full Text Available El presente artículo analiza las prácticas ambientales de los actores sociales que se relacionan con las empresas turísticas del municipio de Valle de Bravo, Estado de México. Cabe mencionar que dichas prácticas se consideraron desde el punto de vista socio- económico, es decir, no sólo como se asumen en cuanto al manejo de los recursos naturales, sino también desde su entorno social y económico. Por ello, a partir de la propuesta metodológica de la teoría de sistemas complejos, se analizan los efectos producidos en los entornos de este destino turístico, lo que permite visualizar deuna manera holística la situación que prevalece para al cuidado del ambiente con la participación de todos los actores sociales de la localidad. Los resultados encontrados muestran una gran debilidaden la implementación de dichas prácticas, ya que éstas, actualmente, son impuestas por el gobierno municipal y no se asumen como una iniciativa de los propios actores que participan en las empresas turísticas. Por esta razón, las condiciones de deterioro ambiental en Valle de Bravo son cada vez mayores. A partir de los resultados generados por este estudio, se formulan recomendaciones de implementación y mejoramiento de las prácticas ambientales que contribuyen a la conservación y al cuidado del ambiente.

  11. Taxonomic position of Pinus uliginosa Neumann as related to other taxa of Pinus mugo complex

    OpenAIRE

    Wiesław Prus-Głowacki; Ewa Bajus; Halina Ratyńska

    2014-01-01

    Studies on taxonomic position of Pinus uliginosa Neumann, P. uncinata Ram. and P. mugo Turra, indicate a significant specificity of P. uliginosa population from Wielkie Torfowisko Batorowskie Peat Bog. Pinus uncinata in respect of genetic similarity is close to Pinus mugo Turra populations from the Tatra Mts. The time of divergence of P. uliginosa from the common ancestor, taking into account Nei's genetic distances, is twice as long as in the case of Pinus uncinata. In the view of the result...

  12. Anomalous needle numbers on dwarf shoots of Pinus mugo and P. uncinata (Pinaceae

    Directory of Open Access Journals (Sweden)

    Krystyna Boratyńska

    2011-01-01

    Full Text Available The frequency of occurrence of abnormal, three- (or more needle dwarf shoots of most southern and central European two-needle pine (Pinus species were studied. No specimens with more than two-needle dwarf shoots were found in a population of P. nigra Arnold subsp. salzmannii (Dunal Franco from the Iberian Peninsula and in two populations of P. uliginosa Neumann from the Sudeten Mountains in Central Europe. Single specimens were found within one population of P. pinaster Aiton from the Iberian Peninsula and among six populations of P. sylvestris L. from the Iberian Peninsula and Central Europe. Abnormal dwarf shoots mostly with three, but also four, five or six needles were found among 24 of 25 surveyed populations of P. mugo Turra and P. uncinata Ramond. The average frequency of specimens with at least one three-needle dwarf shoot was 24% for P. mugo and 20% for P. uncinata. The frequencies of occurrence varied significantly among studied populations and were highest in samples collected from the upper elevational range limits of the species in the mountains and near the northern limits of their ranges. The frequency of abnormal dwarf shoots in the same populations was significantly high in 2-3 consecutive years. Needles from three-needle dwarf shoots were not significantly shorter than those of two-needle shoots.

  13. Bioecology of the fungus Sphaeropsis sapinea dyko & Sutton - agents of pinus species decline

    Directory of Open Access Journals (Sweden)

    Milijašević Tanja

    2002-01-01

    Full Text Available Sphaeropsis sapinea is a cosmopolitan fungus, identified in more than 50 countries of the world, on all continents, but it is primarily the species of warm lands. It is also a polyphagous fungus recorded from 11 coniferous genera. The most endangered and the most frequent host plants are Pinus species - it occurs on 48 pine species, among which the most susceptible are Pinus Radiata, P. nigra, P. sylvestris, P. ponderosa, P. resinosa, P. mugo, P. pinaster and P. elliotti. The greatest damage is caused on the introduced Pinus species and on those cultivated in artificial plantations, shelterbelts and in urban environments. In Yugoslavia S. sapinea is widely distributed both in the continental and in the Mediterranean parts. It was identified from ten pine species and six hosts from other coniferous genera. By the study in our country, the new hosts of this fungus were detected - Pinus jeffrey, P. peuce and P. heldreichii. The most endangered species in our country is Austrian pine, both in urban environments, and in plantations The symptoms of the disease are bud wilt, curling, stunting and necrosis of current year shoots and needles, dieback of top shoots, parts of crown or tree tops, branch and stem bark canker, root collar rot on the young plants in nurseries and their dying. This fungus also prevents seed germination of Pinus species and causes blue sap stain of the freshly cut wood, although sap stain was also observed on standing trees. More rarely it causes root rot and crown wilt of Pinus species. The main symptoms of infection, both of young plants and older trees, are the dieback of current year shoots S. sapinea can penetrate through buds, bark of young shoots and needles. The critical time of infection is the period from mid April to mid May. Then infection mainly penetrates through the bark of young shoots, which results in their dying. Infection through the needles occurs mainly at the time of their sudden growth or during summer

  14. Geochemistry of the thermal springs from San Antonio El Bravo zone, Chihuahua, Mexico; Geoquimica de manantiales termales de la zona de San Antonio El Bravo, Chihuahua, Mexico

    Energy Technology Data Exchange (ETDEWEB)

    Tello Hinojosa, Enrique [Gerencia de Proyectos Geotermoelectricos de la Comision Federal de Electricidad, Morelia (Mexico)

    1996-05-01

    Isotopic and chemical analysis o water produced by 14 springs in the San Antonio El Bravo, Chihuahua, Mexico geothermal area, were carried out in order to establish the geochemical characteristics of the groundwater and to know their interaction with deeper geothermal fluids. We made two samplings of water and gases in 1984 and 1995. The chemical composition of waters produced by the springs in of sodium-bicarbonate-chloride type. It was found, according to the Na:K:Mg relative content, that most hot springs are located in the partial equilibrium zone, whereas the lowest temperature hot springs shift toward the groundwaters domain. The temperature estimated from gas geothermometry was 129 degrees celsius. The isotopic composition at Ojo Caliente and Infiernito springs presents enrichment in {delta}{sup 18}O, product rock-water interaction at high temperature. The Agua Roque spring is located in the line of meteoric waters. Analysis of metals was carried out too, the concentration of gold element is 0.09 mg/l in Ojo Caliente and Infiernito springs, whereas silver, aluminum and iron elements were not detected. The water quality of the springs for agricultural use, is classified between C2-S1, C3-S1, C3- S2, C4-S3 and C4-S4 types, that suggests that only the water from Agua Roque can be used for irrigation. The arsenic element was not detected but the concentration of the boron element is high for irrigation use (2.39 ppm). [Espanol] Los analisis quimicos e isotopicos de agua de 14 manantiales de la zona de San Antonio El Bravo. Chihuahua, Mexico, fueron realizados con el fin de conocer las caracteristicas geoquimicas del acuifero somero y su interaccion con fluidos geotermicos. Se realizaron 2 muestreos tanto de agua como de gases en 1984 y 1995. En ambos muestreos se encontro que la composicion quimica del agua de todos los manantiales es del tipo bicarbonatado-clorurado-sodico. De acuerdo con el contenido relativo de Na:K:Mg el agua de los manantiales mas calientes

  15. El Turismo Residencial en Valle de Bravo, México. Una Interpretación de su Ciclo de Vida

    OpenAIRE

    Deverdun Reyna, Marie Eugenie; Osorio García, Maribel; Iracheta Cenecorta, Alfonso

    2016-01-01

    El turismo residencial en Valle de Bravo (Estado de México) surge a partir de la consolidación de su presa, iniciando un proceso de transformación no sólo paisajística, sino territorial y socioeconómica, el cual además, ha marcado la pauta para llevar a este destino a convertirse en uno de los sitios turísticos más importantes del Estado de México. El presente artículo tiene como objetivo realizar una caracterización territorial y urbana de las etapas que ha tenido Valle de Bravo debido a la ...

  16. Development of a United States-Mexico Emissions Inventory for the Big Bend Regional Aerosol and Visibility Observational (BRAVO) Study.

    Science.gov (United States)

    Kuhns, Hampden; Knipping, Eladio M; Vukovich, Jeffrey M

    2005-05-01

    The Big Bend Regional Aerosol and Visibility Observational (BRAVO) Study was commissioned to investigate the sources of haze at Big Bend National Park in southwest Texas. The modeling domain of the BRAVO Study includes most of the continental United States and Mexico. The BRAVO emissions inventory was constructed from the 1999 National Emission Inventory for the United States, modified to include finer-resolution data for Texas and 13 U.S. states in close proximity. The first regional-scale Mexican emissions inventory designed for air-quality modeling applications was developed for 10 northern Mexican states, the Tula Industrial Park in the state of Hidalgo, and the Popocatépetl volcano in the state of Puebla. Emissions data were compiled from numerous sources, including the U.S. Environmental Protection Agency (EPA), the Texas Natural Resources Conservation Commission (now Texas Commission on Environmental Quality), the Eastern Research Group, the Minerals Management Service, the Instituto Nacional de Ecología, and the Instituto Nacional de Estadistica Geografía y Informática. The inventory includes emissions for CO, nitrogen oxides, sulfur dioxide, volatile organic compounds (VOCs), ammonia, particulate matter (PM) < 10 microm in aerodynamic diameter, and PM < 2.5 microm in aerodynamic diameter. Wind-blown dust and biomass burning were not included in the inventory, although high concentrations of dust and organic PM attributed to biomass burning have been observed at Big Bend National Park. The SMOKE modeling system was used to generate gridded emissions fields for use with the Regional Modeling System for Aerosols and Deposition (REMSAD) and the Community Multiscale Air Quality model modified with the Model of Aerosol Dynamics, Reaction, Ionization and Dissolution (CMAQ-MADRID). The compilation of the inventory, supporting model input data, and issues encountered during the development of the inventory are documented. A comparison of the BRAVO emissions

  17. Trace elements and organic compounds associated with riverbed sediments in the Rio Grande/Rio Bravo basin, Mexico and Texas

    Science.gov (United States)

    Lee, R.W.; Wilson, J.T.

    1997-01-01

    In 1991, the Texas Natural Resource Conservation Commission (TNRCC) was mandated by the Texas Clean Rivers Act (Senate Bill 818) to assess water quality of rivers in Texas. Recent efforts to collect information for the assessment of water quality in the Rio Grande/Rio Bravo Basin have involved Federal agencies on both sides of the 1,248-mile U.S.-Mexico border?U.S. Environmental Protection Agency, U.S. Geological Survey (USGS), Secretaria de Desarollo Social (Secretary for Social Development, Mexico), National Water Commission of Mexico, and International Boundary and Water Commission?as well as State and local agencies in a spirit of international cooperation. Substantial efforts have been made to gather data needed to determine the quality of water and ecological status of the Rio Grande/Rio Bravo, especially at sites along the border (fig. 1). The purpose of this report is to assess selected historical data of trace elements and organic compounds in riverbed sediments of the Rio Grande/Rio Bravo, and of the Pecos River and the Arroyo Colorado in Texas.

  18. Assessment of acute toxicity of the ethanolic extract of Lychnophora pinaster (Brazilian arnica

    Directory of Open Access Journals (Sweden)

    Simone A. Ferreira

    2014-10-01

    Full Text Available Species of the Lychnophora genus are plants native to Brazil, popularly known as "Brazilian arnica" and used in folk medicine as alcoholic and hydro-alcoholic preparations for the treatment of bruises, inflammation, pain, rheumatism and insect bites. The present study aimed to evaluate the safety of the use of Lychnophora pinaster Mart., Asteraceae. Acute toxicity of the crude ethanolic extract was evaluated by administration of the extract by oral route to male and female Swiss mice. A single extract dose of 125, 250 or 500 mg/kg was administered and the effects on spontaneous locomotor activity, exploratory behavior, muscle strength, body weight, food and water consumption, relative organ weight, histology, as well as hematological and biochemical parameters were evaluated. The three doses administered to the animals did not cause muscle tone alterations, but doses of 250 and 500 mg/kg induced a significant inhibition of the spontaneous locomotor activity and exploratory behavior of the animals in open-field test. There was no alteration to hematological parameters and consumption of water and food, body weight variation and organs relative weight. Changes were observed in AST and ALT during assessment of biochemical parameters. The histopathological evaluation showed that the extract provoked cellular alterations, such as vacuolar degeneration and inflammation in kidneys and liver at all doses. Liver morphometric analyses of male and female mice showed that the extract did not have dose-dependent effects. Although females showed a significant increase in inflammatory cells, the effect was not dose-dependent.

  19. Trophic relationships: effect of bacteria, fungi and nematodes interactions on P nutrition from phytate in the rhizosphere of Pinus pinaster seedlings

    OpenAIRE

    2011-01-01

    Aims and Background: Phytate accounts for large P fractions accumulating in soil. However, plants are poorly able to access this source of P because of their low phytate mineralizing capacity. In contrast to plants, some rhizosphere bacteria are able to mineralize phytate. This activity could benefit first to bacterial grow th and plant P availability could be enhanced only through bacterial grazers liberating nutrients locked up in bacterial biomass. We hypothesize that phytase- producing ba...

  20. Existing Opportunities to Adapt the Rio Grande/Bravo Basin Water Resources Allocation Framework

    Directory of Open Access Journals (Sweden)

    Luzma Fabiola Nava

    2016-07-01

    Full Text Available The study of the Rio Grande/Bravo (RGB Basin water allocation demonstrates how the United States (U.S. and Mexico have consolidated a transboundary framework based on water sharing. However, the water supply no longer meets the ever-increasing demand for water or the expectations of different stakeholders. This paper explores opportunities for an enhanced management regime that will address past problems and better examine how to balance demands for a precious resource and environmental needs. Based on an overview of the RGB Basin context and the water allocation framework, as well as a discussion on stakeholders’ ability to achieve solutions, this paper explores three key questions: (1 Does the current binational water allocation framework meet current and future human and environmental needs? (2 How can the U.S.-Mexico water allocation framework be adapted to balance social and environmental water demands so it can support and preserve the RGB Basin ecosystem? (3 What are the main opportunities to be explored for expanding the U.S.-Mexico water resources allocation framework? The U.S.-Mexico water resources framework is subject to broad interpretation and may be adapted to the circumstances taking the fullest advantage of its flexibility. Policy recommendations highlight the existing flexibility of the binational framework, the potential to move forward with an ad hoc institutional arrangement, and the creation of political will to achieve change through stakeholders recommendations.

  1. Composition of pectic polysaccharides in a Portuguese apple (Malus domestica Borkh. cv Bravo de Esmolfe

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    Ana S. Silva

    2014-08-01

    Full Text Available Malus domestica Borkh. cv Bravo de Esmolfe is a typical Portuguese apple cultivar classified as Protected Designation and Origin (PDO. It is a traditional product produced under strict conditions and labelled with a specific law protected designation. This cultivar presents quite good sweetness and flavor. The monosaccharide composition of the pectic polysaccharides from this traditional apple is herein reported for the first time. Based on the molar ratios obtained from the sugar composition, the presumable pectin structure could be inferred. The cell-wall polysaccharides present in the alcohol-insoluble residue (AIR of unpeeled BE apple were sequentially fractionated. In addition, pectic material was also extracted by citric acid treatment prior to heat extraction at acidic pH. The water soluble pectin, imidazole soluble pectin and sodium carbonate soluble pectin account for 44, 16 and 40 % of the AIR, respectively. The pectic polysaccharides extracted in the presence of citric acid had lower galacturonic acid content and higher neutral sugars content. The homogalacturonan (HG and less-substituted rhamnogalacturonan (RG domains are extracted first. Pectin treated with citric acid has been shown to contain more substituted polymers, especially RG-I. In addition, the relatively higher Xylose/Galacturonic acid ratio found in the citric acid extract demonstrates that the xylogalacturonan (XG domain presumably is present in the pectic material of the unpeeled BE apple.

  2. Fossil records of subsection Pinus (genus Pinus, Pinaceae) from the Cenozoic in Japan.

    Science.gov (United States)

    Yamada, Toshihiro; Yamada, Mariko; Tsukagoshi, Minoru

    2014-03-01

    Extant pines of subsection Pinus (section Pinus, genus Pinus, Pinaceae) are predominantly distributed in Eastern Asia. However, the extent of diversification in the section has yet to be fully clarified. We reviewed fossil records of subsection Pinus from Japan and collected permineralized materials, in which anatomical details are preserved for better understanding of the diversification. Our results suggest that this subsection appeared in Japan no earlier than the Middle Eocene, with extant species (i.e., Pinus densiflora and Pinus thunbergii) appearing around the beginning of the Pleistocene. Pinus fujiii (Early Miocene to Early Pleistocene) is inferred to have a close affinity to P. thunbergii based on the medial arrangement of its leaf resin canals. Additionally, P. fujiii has a similar cone morphology to those of extant species living in China, bridging the morphological gap between P. thunbergii and Chinese relatives of P. thunbergii as inferred by molecular phylogenetic analyses. Our results also suggest that taxonomic revisions of Pinus miocenica and Pinus oligolepis are required among the Japanese fossil species reported to date.

  3. Wind pollination in Pinus roxburghii

    Institute of Scientific and Technical Information of China (English)

    Vinod Prasad KHANDURI; Chandra Mohan SHARMA

    2007-01-01

    Stigmatic pollen load and pollen concentration in the air were studied in the natural population of Pinus roxburghii at Ashtavakra (900 m asl), in the Pauri forest division of Garhwal Himalaya, India. The results reflect diurnal pollen occurrence in P. roxburghii, with the strong significant correlations between pollen concentrations in the air and wind speed, air temperature and relative air humidity. A significant correlation was also observed between microsporangium dehiscence and pollen occurrence in the air. The maximum concentration of pollen grains in the air and higher rates of pollen deposition onto the megasporophylls were between 12 pm and 16 pm of the day, which conforms the best time for pollination in a day in P. roxburghii. The receptivity of Ovulate strobili varied from 3 to 5days, however, the bagged strobili remained receptive up to 6 days.

  4. IpsiHand Bravo: an improved EEG-based brain-computer interface for hand motor control rehabilitation.

    Science.gov (United States)

    Holmes, Charles Damian; Wronkiewicz, Mark; Somers, Thane; Liu, Jenny; Russell, Elizabeth; Kim, DoHyun; Rhoades, Colleen; Dunkley, Jason; Bundy, David; Galboa, Elad; Leuthardt, Eric

    2012-01-01

    Stroke and other nervous system injuries can damage or destroy hand motor control and greatly upset daily activities. Brain computer interfaces (BCIs) represent an emerging technology that can bypass damaged nerves to restore basic motor function and provide more effective rehabilitation. A wireless BCI system was implemented to realize these goals using electroencephalographic brain signals, machine learning techniques, and a custom designed orthosis. The IpsiHand Bravo BCI system is designed to reach a large demographic by using non-traditional brain signals and improving on past BCI system pitfalls.

  5. Medical status of Marshallese accidentally exposed to 1954 Bravo fallout radiation: January 1988 through December 1991

    Energy Technology Data Exchange (ETDEWEB)

    Howard, J.E.; Heotis, P.M.; Scott, W.A.; Adams, W.H.

    1995-07-01

    The purpose of this report is to disseminate information concerning the medical status of 253 Marshallese exposed to fallout radiation in 1954. This report discusses the medical care provided and the medical findings for the years 1988-1991. Details of the BRAVO thermonuclear accident that caused the exposure have been published, and a 1955 article in the Journal of the American Medical Association describing the acute medical effects in the exposed population remains a definitive and relevant description of events. Participation in the Marshall Islands Medical Program by the exposed Marshallese is voluntary. In the spring and fall of each year, medical surveillance is provided to exposed and unexposed cohorts. Examinations performed include: a cancer-related examination as defined by the American Society, an annual thyroid examination and thyroid function testing, serum prolactin testing looking for pituitary tumors, annual blood counts to include platelets, and evaluation for paraneoplastic evidence of neoplasms. This report details the medical program, medical findings, and thyroid surgery findings. Deaths (4 exposed and 10 nonexposed) that occurred during the reporting period are discussed. There is a mild but relatively consistent depression of neutrophil, lymphocyte, and platelet concentrations in the blood of the exposed population. This depression appears to be of no clinical significance. Thyroid hypofunction, either clinical or biochemical, has been documented as a consequence of radiation exposure in 14 exposed individuals. Previously, one other exposed person was diagnosed with basal cell carcinoma. During this reporting period, a thyroid nodule was identified in an individual who was in utero during the exposure. Upon pathologic review, the nodule was diagnosed as occult papillary carcinoma.

  6. Taxonomic position of Pinus uliginosa Neumann as related to other taxa of Pinus mugo complex

    Directory of Open Access Journals (Sweden)

    Wiesław Prus-Głowacki

    2014-01-01

    Full Text Available Studies on taxonomic position of Pinus uliginosa Neumann, P. uncinata Ram. and P. mugo Turra, indicate a significant specificity of P. uliginosa population from Wielkie Torfowisko Batorowskie Peat Bog. Pinus uncinata in respect of genetic similarity is close to Pinus mugo Turra populations from the Tatra Mts. The time of divergence of P. uliginosa from the common ancestor, taking into account Nei's genetic distances, is twice as long as in the case of Pinus uncinata. In the view of the results the supposition that Pinus uliginosa from Wielkie Torfowisko Batorowskie Peat Bog is a marginal population of P. uncinata can be excluded, because of their significant difference in genetic structure. The presumable reasons of specificity of P. uliginosa population are disscused.

  7. a Review of Late Holocene Fluvial Systems in the Karst Maya Lowlands with Focus on the Rio Bravo, Belize

    Science.gov (United States)

    Beach, T.; Luzzadder-Beach, S.; Krause, S.; Doyle, C.

    2015-12-01

    The Maya Lowlands is mostly an internally draining karst region with about 400 m of regional relief. Fluvial and fluviokarst systems drain the edges of this landscape either from low limestone uplands or igneous and metamorphic complexes. Thus far most fluvial research has focused around archaeology projects, and here we review the extant research conducted across the region and new research on the transboundary Rio Bravo watershed of Belize and Guatemala. The Rio Bravo drains a largely old growth tropical forest today, but was partly deforested around ancient Maya cities and farms from 3,000 to 1000 BP. Several studies estimate that 30 to 40 percent of forest survived through the Maya period. Work here has focused on soils and sediment movement along slope catenas, in floodplain sites, and on contributions from groundwater with high dissolved loads of sulfate and calcium. We review radiocarbon dates and present new dates and soil stratigraphy from these sequences to date slope and floodplain movement, and we estimate ancient land use from carbon isotopic and pollen evidence. Aggradation in this watershed occurred by flooding, gypsum precipitation, upland erosion, and ancient Maya canal building and filling for wetland farming. Soil erosion and aggradation started at least by 3,000 BP and continued through the ancient Maya period, though reduced locally by soil conservation, post urban construction, and source reduction, especially in Maya Classic period from 1700 to 1000 BP.

  8. Socialisation et dépendance. Théories de l’impérialisme chez Luciano Ferrari Bravo et Giovanni Arrighi

    OpenAIRE

    Melegari, Diego

    2016-01-01

    L’article compare les positions de Luciano Ferrari Bravo et Giovanni Arrighi à la fin des années 1970, notamment en ce qui concerne la théorie de l’impérialisme et la relation théorie-politique.

  9. Contrafuegos o el resurgimiento de los núcleos agrarios. (Reflexiones a propósito del V informe de Gobierno - Región Valle de Bravo

    Directory of Open Access Journals (Sweden)

    Sergio Zamorano Camiro

    2005-01-01

    Full Text Available La precarización de la vida campesina en los núcleos agrarios de la región Valle de Bravo puede ser remontada reactivando su estructura legal de organización. Esta constituye un importante activo de los actores colectivos que ha sido olvidado por los gobiernos y que debe ser movilizado como capital social para fomentar el desarrollo local. El núcleo agrario de Bosencheve, en el municipio de Villa de Allende fue la sede elegida por el Lic. Arturo Montiel Rojas para informar de los efectos de su gestión gubernamental en la región Valle de Bravo, que comprende a los municipios de Amanalco, Donato Guerra, Ixtapan del Oro, Otzoloapan, Santo Tomás, Valle de Bravo, Villa de Allende, Villa Victoria y Zacazonapan. Esta elección singular de la sede (un núcleo agrario, relacionada con el dato de COESPO-INEGI (2000 acerca del elevado grado de marginación (1.35 que padecen los habitantes de la región Valle de Bravo nos sugiere hacer con nuestros lectores las reflexiones que siguen.

  10. Water Management for Competing Uses: Environmental Flows in the Transboundary Rio Grande/Rio Bravo

    Science.gov (United States)

    Sandoval Solis, S.; McKinney, D. C.

    2011-12-01

    Introduction Due to high water demand, the scarcity of water, and the complexity of water allocation, environmental flows have not been considered as an integral part of the water management in the Rio Grande/Rio Bravo transboundary basin. The Big Bend reach is located between the cities of Presidio/Ojinaga to Amistad international reservoir, along the main stream (Fig. 1). Important environmental habitats such as the Big Bend National and State Park in the U.S., the Maderas del Carmen, Cañon de Santa Elena and Ocampo natural reserved areas in Mexico are ecologically threatened because of the lack of environmental water management policies. Several efforts have been undertaken by scientists, government agencies and NGOs to determine the environmental flows for this reach and water management policies that can provide these flows. Objective The objective of this research is to describe a water management policy that can conciliate environmental and human water uses in the Big Bend region. In other words, define a policy that can provide environmental flows without harming water supply for stakeholders or increasing flood risk, within legal and physical constraints of the system. Methodology First, the system was characterized identifying water users, hydraulic infrastructure, and water allocation according to state, federal and international regulations. Second, a hydrograph for environmental flows was proposed that mimics the hydrologic characteristics of the prior dam alteration. Third, a water planning model was constructed to evaluate alternative policies. Fourth, the water management is proposed to provide environmental restoration flows from Luis L. Leon reservoir. This policy considers mechanisms that reduce flooding and drought risks, while meting national and international water regulations. Results Three types of natural flow regimes are considered: (1) median flows aimed to provide the base flow in the region, (2) high flows to provide transversal

  11. Pinus taeda AND Pinus oocarpa PLYWOOD MANUFACTURING WITH FENOL-FORMALDHEYDE RESIN

    Directory of Open Access Journals (Sweden)

    Setsuo Iwakiri

    2002-01-01

    Full Text Available The objective of this study was to evaluate the quality of the plywood, manufactured from Pinus taeda and Pinus oocarpa, with 20 and 24 years old respectively, using three differentformulations of the fenol-formaldheyde resin. The results of the glue line shearing tests not showed statistical differences between the species and three resin formulations. In the hot water test, all of the boards were classified as “BR” and, the boards of the Pinus oocarpa produced with formulations (1and (3, were classified as “WBP”. The different resin formulations not influenced on the modulus ofelasticity (MOE and modulus of rupture (MOR The boards of Pinus taeda with formulations (1 and(2 showed higher values of MOE in comparison of the Pinus oocarpa. The MOR of the Pinus oocarpa withformulation (2 was higher than Pinus taeda. The mechanical properties of the plywood wereprobably influenced by wood variability related to sapwood and hartwood, thickness of growth ringsand, springwood and summerwood.

  12. Bravo capsule system optimizes intragastric pH monitoring over prolonged time: Effects of ghrelin on gastric acid and hormone secretion in the rat

    Institute of Scientific and Technical Information of China (English)

    Tobias Rudholm; Per Mikael Hellstrom; Elvar Theodorsson; Colin Allan Campbell; Peter Geoffrey McLean; Erik Naslund

    2008-01-01

    AIM: To evaluate measurements of intragastric pH with the Bravo capsule system over a prolonged time. METHODS: A Bravo capsule was placed inside the rat gastric body and pH was studied for periods up to five consecutive days. For comparison, a gastric fistula model was used. Effects of ghrelin and esomeprazole, with or without pentagastrin, on gastric pH were studied. In addition, effects of esomeprazole on plasma ghrelin, gastrin and somatostatin were analyzed. RESULTS: All rats recovered after surgery. The average 24-h pH during free feeding was 2.3±0.1 (η = 20) with a variation of 18%±6% over 5 d. Ghrelin, 2400 pmol/kg, t.i.d, increased pH from 1.7± 0.1 to 3.1±0.3 (P<0.01) as recorded with the Bravo system. After esomeprazole (1 mg/kg, 3 mg/kg and 5 mg/kg) there was a dose-dependent pH increase of maximally 3.4±0.1, with day-to-day variation over the entire period of 8%±3%. The fistula and pH studies generated similar results. Acid inhibition with esomeprazole increased plasma ghrelin from 10±2 pmol/L to 65±26 pmol/L (P<0.001), and somatostatin from 10±2 pmol/L to 67±18 pmol/L (P <0.001). CONCLUSION: pH measurements with the Bravo capsule are reliable, and comparable to those of the gastric fistula model. The Bravo system optimizes accurate intragastric pH monitoring over prolonged periods and allows both short- and long-term evaluation of effects of drugs and hormones.

  13. Knowledge about useful entomofauna in the county of La Purísima Palmar de Bravo, Puebla State, Mexico

    Directory of Open Access Journals (Sweden)

    D. H. Zetina

    2007-06-01

    Full Text Available Eighteen species of edible insects belonging to 15 families of six orders of Insecta were recorded in the county of La Purísima Palmar de Bravo in Puebla State, Mexico. They had the following taxonomic distribution among the different families: Lygaeidae, Pentatomidae, Membracidae, Cercopidae, Psocidae, Melolonthidae, Curculionidae, Cossidae, Megathymidae, Pyralidae, Geometridae and Apidae, all with a single recorded species; the families Acrididae, Formicidae and Vespidae with two species in each family. The latter were the most abundant families, and Hymenoptera was the most salient order with five species. Three species were reported as new registers of edible insects for Mexico and also for the world. The nutritive value of insects in terms of macro and micronutrients is discussed.

  14. Desigualdades, diferencias y experiencias de (no pertenencia en educación, de Ana Bravo-Moreno (Ed.

    Directory of Open Access Journals (Sweden)

    Ivy Jacaranda Jasso Martínez

    2016-01-01

    Full Text Available El texto que nos presenta Ana Bravo-Moreno es una colección de capítulos que tienen como eje transversal el tema de la desigualdad generada a partir de la/s diferencia/s, analizando cómo se producen, negocian y cambian estas en contextos específicos. Se trata de un tema en boga, que se ha venido discutiendo en la última década, especialmente respecto a las diferentes posturas que han evidenciado y discutido la manera y estrategias en que se vive y experimenta la diversidad cultural en el aula y en la escuela. El libro consta de ocho capítulos, más la introducción, donde se pasa revista de diferentes casos (España, Argentina, Estados Unidos, Grecia, y cada capítulo aborda con diferente profundidad la temática.

  15. Cloning and Sequence Analysis of Full-Length cDNA of Phenylalanine Ammonia-Lyase of Pinus Massoniana%马尾松苯丙氨酸解氨酶基因cDNA全长克隆与序列分析

    Institute of Scientific and Technical Information of China (English)

    曹福祥; 王猛; 龙绛雪

    2010-01-01

    苯丙氨酸解氨酶(Phenylalanine ammonia-lyase,PAL)是连接植物初级代谢和次级代谢途径关键酶、限速酶,催化苯丙氨酸转化为肉桂酸,促进黄酮、木质素等次生代谢产物的合成,在植物抗病过程中有重要意义.采用RT-PCR和RACE技术,从马尾松中克隆到PAL基因的cDNA全长.此cDNA全长2 700 bp,包括2 154 bp的完整ORF,编码717个氨基酸的蛋白,相对分子质量与等电点分别为78 200和5.81.其推导的蛋白序列与火炬松(Pinus taeda)、欧洲赤松(Pinus sylvestris)和海岸松(Pinus pinaster)的苯丙氨酸解氨酶同源性分别为98.2%、99.4%和97.8%.

  16. Carbon content of forest floor and mineral soil in Mediterranean Pinus spp. and Oak stands in acid soils in Northern Spain

    Energy Technology Data Exchange (ETDEWEB)

    Herrero, C.; Turrión, M.B.; Pando, V.; Bravo, F.

    2016-07-01

    Aim of the study: The aim of the study was to determine the baseline carbon stock in forest floor and mineral soils in pine and oak stands in acid soils in Northern Spain. Area of study: The study area is situated in northern Spain (42° N, 4° W) on “Paramos y Valles” region of Palencia. aterial and methods: An extensive monitoring composed of 48 plots (31 in pine and 17 in oak stands) was carried out. Litter layers and mineral soil samples, at depths of 0-30 cm and 30-60 cm, were taken in each plot. An intensive monitoring was also performed by sampling 12 of these 48 plots selected taken in account species forest composition and their stand development stage. Microbial biomass C (CMB), C mineralization (CRB), and soil organic C balance at stand level were determined in surface soil samples of intensive monitoring. Main results: No differences in soil C content were detected in the two forest ecosystems up to 60 cm depth (53.0±25.8 Mg C ha-1 in Pinus spp. plantations and 60.3±43.8 Mg C ha-1 in oak stands). However, differences in total C (CT), CMB and CRB were found in the upper 10 cm of the soils depending on the stand development stage in each species forest composition (Pinus nigra, Pinus pinaster, Pinus sylvestris and Quercus pyrenaica). Plots with high development stage exhibited significant lower metabolic quotient (qCO2), so, meant more efficient utilization of C by the microbial community. The C content in the forest floor was higher in pine stands (13.7±0.9 Mg C ha-1) than in oak stands (5.4±0.7 Mg C ha-1). A greater turnover time was found in pine ecosystems vs. oak stands. In contrast, forest floor H layer was nonexistent in oak stands. Research highlights: Results about litterfall, forest floor and mineral soil dynamics in this paper can be used strategically to reach environmental goals in new afforestation programs and sustainable forest management approaches. (Author)

  17. Characterization of the volatile fraction emitted by phloems of four pinus species by solid-phase microextraction and gas chromatography-mass spectrometry.

    Science.gov (United States)

    Santos, A M; Vasconcelos, T; Mateus, E; Farrall, M H; Gomes da Silva, M D R; Paiva, M R; Branco, M

    2006-02-10

    Pine forests constitute some of the most important renewable resources supplying timber, paper and chemical industries, among other functions. Characterization of the volatiles emitted by different Pinus species has proven to be an important tool to decode the process of host tree selection by herbivore insects, some of which cause serious economic damage to pines. Variations in the relative composition of the bouquet of semiochemicals are responsible for the outcome of different biological processes, such as mate finding, egg-laying site recognition and host selection. The volatiles present in phloem samples of four pine species, P. halepensis, P. sylvestris, P. pinaster and P. pinea, were identified and characterized with the aim of finding possible host-plant attractants for native pests, such as the bark beetle Tomicus piniperda. The volatile compounds emitted by phloem samples of pines were extracted by headspace solid-phase micro extraction, using a 2cm 50/30mm divinylbenzene/carboxen/polydimethylsiloxane table flex solid-phase microextraction fiber and its contents analyzed by high-resolution gas chromatography, using flame ionization and a non polar and chiral column phases. The components of the volatile fraction emitted by the phloem samples were identified by mass spectrometry using time-of-flight and quadrupole mass analyzers. The estimated relative composition was used to perform a discriminant analysis among pine species, by means of cluster and principal component analysis. It can be concluded that it is possible to discriminate pine species based on the monoterpenes emissions of phloem samples.

  18. Fire Occurrence Environments in Pinus pumila Forests

    Institute of Scientific and Technical Information of China (English)

    2010-01-01

    In recent years, many serious forest fires occurred in precious Pinus pumila forests in Daxing'anling Mountains of Heilongjiang Province and Inner Mongolia. But up to now, there is still a lack of proper understanding of fire occurrence environments in P. pumila forests. In present paper, we investigated and studied the fire occurrence environments. The results showed that fires in P. pumila forests had their own special fire environments. Abundant fuel, drought weather, dry thunder and high altitude terrai...

  19. Two new antitumor diterpenes from Pinus sylvestris

    Institute of Scientific and Technical Information of China (English)

    Qiang Wang; Zhang Rong; Jie Teng; Yoshihisa Takaishi; Hong Quan Duan

    2008-01-01

    Two new diterpenes,15-ethyl- 18-methyl pinifolate (1)and 18-hydroxy-labda-8(17),13E-dien-15-acetate (2),were isolated fromthe needles of Pinus sylvestris.Their structures were elucidated by spectroscopic methods,including 2D-NMR spectra.Compound1 exhibited the significant eytotoxic activity against the human carcinoma cell lines Hela,SK-N-SH and BEL-7402 in vitro.

  20. Historical events associated with fallout from Bravo Shot-Operation castle and 25 y of medical findings

    Energy Technology Data Exchange (ETDEWEB)

    Cronkite, E.P.; Conard, R.A.; Bond, V.P. [Brookhaven National Labs., Upton, NY (United States)

    1997-07-01

    The events prior to Bravo Shot-Operation Castle that led to a decision not to evacuate the Marshallese prior to testing the thermonuclear bombs are presented as are the actions taken after the fallout incident in evacuating the exposed Marshallese and the military personnel. The initial medical effects (findings during first 6 wk after exposure) are briefly described and are followed by description of long term effects, namely, induction of one case or fatal acute myeloid leukemia and a large number of thyroid tumors (benign and malignant) in addition to hypothyroidism in adults and children and two cases of cretinism. The hypothyroidism and cretinism responded well to administration of oral thyroxine. During the first 25 y, there was also much unrest and political agitation initiated by exposed and unexposed Marshallese who were very unhappy as a result of relocation and inability to return to their homelands and feeling that all illness and deaths were due to the mysterious radiation, which they understandably did not understand. The difficulties in part were ameliorated by financial aid from the U.S. Congress. In view of one of us (EPC), no one agency or person in the U.S. Government was willing to take the responsibility for care of the Marshallese and its financing. The exposed and non-exposed Marshallese had their lifestyle changed, some of their homelands made uninhabitable for several years and could aptly be called {open_quotes}nuclear nomads,{close_quotes} an expression coined by others. 14 refs., 3 tabs.

  1. Effects of SO/sub 2/ on the germination of conifer pollen. [Pinus mugo; Pinus nigra; Pinus sylvestris

    Energy Technology Data Exchange (ETDEWEB)

    Keller, T.; Beda, H.

    1984-01-01

    Collections on agar of fresh and stored pollen grains of Pinus mugo, P. nigra and P. sylvestris were fumigated with SO/sub 2/ for 16 or 24 h. The ability of pollen of P. mugo and P. sylvestris to germinate was decreased by storage but sensitivities to SO/sub 2/ were not greatly altered. Germination was inhibited consistently by atmospheres with 0.225 ppm SO/sub 2/. At 0.075 ppm - a typical ambient concentration during temperature inversions - P. nigra and P. sylvestris were more sensitive than P. mugo which responded in the same way as pollen of Abies alba.

  2. Spontaneous Hybridization between Pinus mugo and Pinus sylvestris at the Lithuanian Seaside

    DEFF Research Database (Denmark)

    Danusevicius, Darius; Marozas, Vitas; Brazaitis, Gediminas;

    2012-01-01

    We address the problem of spontaneous hybridization between an exotic species Pinus mugo and the native/local P. sylvestris at the seaside spit of Kursiu Nerija in Lithuania. The objective was to identify spontaneous hybrids between P. mugo and P. sylvestris based on morphology traits among the i...

  3. Relações de produtividade, área folhear e alternância na macieira bravo de esmolfe: Yield efficiency, leaf area and alternate bearing indices of apple cv. bravo de esmolfe

    Directory of Open Access Journals (Sweden)

    Alberto Santos

    2008-01-01

    Full Text Available A macieira Bravo de Esmolfe é a mais conhecida e valorizada das variedades regionais, mas o conhecimento técnico disponível é escasso e torna-se necessário aprofundá-lo, em especial no que se refere às formas de gestão e controlo de uma das suas características mais negativas, a forte tendência para alternar as produções. Assim, pretendeu-se identificar algumas relações de produtividade que não desencadeiem alternância, quando cultivada no porta-enxerto ananicante EMLA9, que tem sido considerado entre os melhores actualmente disponíveis para o seu cultivo em regime intensivo. Para o efeito foram seleccionadas 30 macieiras de 8 anos, de estatura semelhante, e que apresentaram diferentes níveis de produtividade no ano de 2004. Em Novembro de 2004 foram recolhidas as folhas e medida a sua área, e contabilizadas as respectivas inflorescências em Abril de 2005, o que forneceu uma perspectiva da produção para essa campanha. Tendo em conta tais valores, e recorrendo ao seu historial produtivo, foram calculados alguns parâmetros que permitiram a análise do comportamento e regularidade de produção das macieiras seleccionadas. Os resultados obtidos nesta primeira abordagem sugerem que intervalos de produtividade da ordem de 8 a 11 g de maçã por folha durante a campanha, a que correspondem 20 a 30 maçãs por cm de diâmetro do tronco, bem como valores entre 10 e 15 folhas por maçã produzida, evitam a alternância de produção, desde que sejam feitas mondas atempadas, conseguindo-se assim bom equilíbrio produção/vegetação.The Bravo de Esmolfe apple tree is the most reputed and valued regional variety, but the available technical knowledge about it does not suffice. It must be deepened, especially on what concerns tools for management and control of one of this cultivar’s most negative characteristics, the strong tendency to alternate bearing. Therefore, the aim of this study was to identify some yield relations that

  4. 乔松Pinus wallichiana与不丹松Pinus bhutanica之初步辨析%A preliminary analysis of the difference between Pinus wallichiana and Pinus bhutanica

    Institute of Scientific and Technical Information of China (English)

    郑维列; 徐阿生

    2003-01-01

    根据最新的文献资料,初步分析了乔松Pinus wallichiana与不丹松Pinus bhutanica在一年生小枝、针叶(主要是树脂道)、球果、种鳞、鳞脐和种子等六个方面的性状区别特征,结合以前对藏东南和藏南地区单维管束松树的调查所掌握的材料,认为不丹松是从乔松中分出来的;它们的分布区存在重叠,但各自构成的群落具有较大的生态学差异;通过性状差异性质的辨析,讨论了不丹松种级地位成立所需的关键特征,初步得出不丹松种级地位难以成立的结论.

  5. Estados del ego, transacciones, juegos y comunicación no verbal en "Luna nueva", Tener y no tener" y "Río Bravo" de Howard Hawks

    OpenAIRE

    Requeijo Rey, Paula

    2012-01-01

    Esta Tesis doctoral pretende estudiar la comunicación intrapersonal e interpersonal en tres de las películas del director Howard Hawks: Luna nueva (1940), Tener y no tener (1944) y Río Bravo (1959). Hemos elegido estos tres filmes por los actores que intervienen en ellos y la calidad de sus interpretaciones, por sus guionistas y porque son representativos de tres de los géneros principales que el realizador norteamericano trabajó: comedia (Luna nueva), aventuras (Tener y no tener) y wester...

  6. Sedimentary record of water column trophic conditions and sediment carbon fluxes in a tropical water reservoir (Valle de Bravo, Mexico).

    Science.gov (United States)

    Carnero-Bravo, Vladislav; Merino-Ibarra, Martín; Ruiz-Fernández, Ana Carolina; Sanchez-Cabeza, Joan Albert; Ghaleb, Bassam

    2015-03-01

    Valle de Bravo (VB) is the main water reservoir of the Cutzamala hydraulic system, which provides 40% of the drinking water consumed in the Mexico City Metropolitan Area and exhibits symptoms of eutrophication. Nutrient (C, N and P) concentrations were determined in two sediment cores to reconstruct the water column trophic evolution of the reservoir and C fluxes since its creation in 1947. Radiometric methods ((210)Pb and (137)Cs) were used to obtain sediment chronologies, using the presence of pre-reservoir soil layers in one of the cores as an independent chronological marker. Mass accumulation rates ranged from 0.12 to 0.56 g cm(-2) year(-1) and total organic carbon (TOC) fluxes from 122 to 380 g m(-2) year(-1). Total N ranged 4.9-48 g m(-2) year(-1), and total P 0.6-4.2 g m(-2) year(-1). The sedimentary record shows that all three (C, N and P) fluxes increased significantly after 1991, in good agreement with the assessed trophic evolution of VB and with historic and recent real-time measurements. In the recent years (1992-2006), the TOC flux to the bottom of VB (average 250 g m(-2) year(-1), peaks 323 g m(-2) year(-1)) is similar to that found in highly eutrophic reservoirs and impoundments. Over 1/3 of the total C burial since dam construction, circa 70,000 t, has occurred in this recent period. These results highlight the usefulness of the reconstruction of carbon and nutrient fluxes from the sedimentary record to assess carbon burial and its temporal evolution in freshwater ecosystems.

  7. Germinação de aquênios de arnica (Lychnophora pinaster Mart. armazenados em diferentes condições Seed germination of arnica (Lychnophora pinaster Mart stored at different conditions

    Directory of Open Access Journals (Sweden)

    Paulo Régis Bandeira de Melo

    2007-02-01

    Full Text Available A antecipação da coleta pode contribuir para redução da rápida deterioração das sementes no campo, preservando, assim, sua qualidade fisiológica. No caso da arnica (Lychnophora pinaster Mart., a formação dos aquênios ocorre de maneira desuniforme na planta, sendo dristibuída ao longo do período de frutificação. Assim, em se tratando de uma espécie medicinal não domesticada, ameaçada de extinção, e tendo seu potencial genético ainda pouco estudado, urge a necessidade de monitoramento do comportamento dos aquênios no armazenamento, visando garantir sua reprodução e conseqüente preservação. Esse trabalho foi conduzido com a finalidade de se verificar o desempenho germinativo de aquênios de arnica coletados em dois estádios de maturação e armazenados em diferentes condições. Os aquênios utilizados foram provenientes do município de Itumirim, MG, tendo a coleta sido realizada em dezembro de 2003, em função das características morfológicas dos capítulos, constituindo, assim, dois estádios de maturação. Após a coleta, os aquênios foram submetidos à secagem, pré-limpeza e separados com auxílio de um soprador vertical. Os aquênios foram acondicionados em dois tipos de embalagens (papel ou plástico e armazenados em diferentes ambientes (câmara fria a 10ºC/50%UR ou ambiente de laboratório, por um período de 6 meses. O teste de germinação foi conduzido em BOD ajustada à temperatura alternada 20-30ºC e fotoperíodo de 12 horas. O substrato utilizado foi o papel mata-borrão e os aquênios foram dispostos em caixas plásticas tipo gerbox. Foram utilizadas 4 repetições de 100 aquênios para cada tratamento. A análise sanitária foi efetuada pelo método "Blotter test" com 4 repetições de 50 aquênios por tratamento. As avaliações da umidade, da qualidade fisiológica e sanitária foram realizadas aos 0, 2, 4 e 6 meses. Pelos resultados, verificou-se que não houve diferença entre as quatro condi

  8. Spontaneous Hybridization between Pinus mugo and Pinus sylvestris at the Lithuanian Seaside: A Morphological Survey

    OpenAIRE

    Darius Danusevičius; Vitas Marozas; Gediminas Brazaitis; Raimundas Petrokas; Knud Ib Christensen

    2012-01-01

    We address the problem of spontaneous hybridization between an exotic species Pinus mugo and the native/local P. sylvestris at the seaside spit of Kursiu Nerija in Lithuania. The objective was to identify spontaneous hybrids between P. mugo and P. sylvestris based on morphology traits among the individuals naturally regenerating at the seaside spit. The field inventory was carried out over the entire Lithuanian part of the spit, and 200 individuals morphologically intermediate between P. sylv...

  9. Genetic Analysis of Pinus sylvestris L. and Pinus sylvestris forma turfosa L. Using RAPD Markers

    Directory of Open Access Journals (Sweden)

    Beáta ÁBRAHÁM

    2010-03-01

    Full Text Available The purpose of the present study was to determine the level of genetic diversity within and among Ciuc basin, Romania (populations from Mohos and Luci raised bogs in Harghita Mountain and Sumuleu in Ciuc Mountain Pinus sylvestris populations using molecular markers. Two of populations (Mohos and Luci seems to be the descendants that survived the continental glaciation. Genetic diversity was analyzed by RAPD (Random Amplified Polymorphic DNA. Nine primers were selected for analysis, which generated reproducible bands. On base of presence or absence of homologues bands Nei’s gene diversity, the percentage of polymorphic loci and Nei’s unbiased genetic distance were calculated. The level of genetic variation among populations was found to be low. For both populations the variation values among populations were higher than within populations. The fossil records and geological historical data explain the extremely low genetic diversity of this species. Pinus sylvestris experienced strong bottlenecks during its evolutionary history, which caused the loss of genetic variation. Genetic drift and breeding in post-bottlenecked small populations may be the major forces that contribute to low genetic diversity and genetic differentiation of populations. Human activities may have accelerated the loss of genetic diversity in Pinus sylvestris.

  10. [Individual biomass of natural Pinus densiflora].

    Science.gov (United States)

    Wang, C; Jin, Y; Jin, C; Liu, J; Jin, Y

    2000-02-01

    The aboveground biomass of individuals with different growth potentials in natural Pinus densiflora forest with different stand densities was measured in Yanbian, Jilin Province. The variation of individual biomass affected by densities was in order of dominant tree branch > needle > bark. The biomass components of P. densifliora with different growth potentials varied markedly with the approaching of density class III, and the change of intermediate trees was similar to the whole stand. The vertical distributions of biomass of different trees were different from each other, but all showed that the biomass of trunks and barks was mainly distributed below 6 m high from ground, that of branches was within 6-10 m high, that of needles was uniform in the upper, middle and lower layers, and that of branches and needles in upper layer was least affected by density.

  11. RHIZOGENIC RESPONSE OF PINUS MAXIMARTINEZII RZEDOWSKI

    Directory of Open Access Journals (Sweden)

    M. C. Ojeda-Zacaría

    2015-11-01

    Full Text Available El piñón azul o maxi piñónPinus maximartineziiRzedowski es una especie endémica en peligro de extinción debido a la alteración de sus poblaciones en su hábitat natural y que ha sobrevivido a una restricción genética extrema, además es considerado el más raro de los pinos piñoneros. En este trabajo se evaluó la respuesta rizogénica de brotes dePinus maximartinezii, obtenidos a partir de embriones cigóticos regenerado vía organogénesis, los cuales se sometieron a tratamientos de enraizamientoin vitro. La mayor respuesta de enraizamiento se presentó con pulso de 24 h en presencia de 2.0 mg L-1 de IBA en los medios de cultivo DCR, GD y MS, al 100 y 50% de su concentración original más 0.5% de carbón activado por L-1. Después de ocho semanas se evaluó la variable conforme al número de brotes con raíces, la prueba de X2 mostró que el enraizamiento es independiente de los medios de cultivo así como la concentración de los mismos y dependiente al tiempos de pulso. La formación de raíces obtenidas en los brotes permite proponer esta especie con capacidad para ser propagadain vitro.

  12. Fire effects in Pinus uncinata Ram plantations

    Directory of Open Access Journals (Sweden)

    Adrián Cardil Forradellas

    2016-04-01

    Full Text Available Aim of study: Understanding fire ecology of main forest species is essential for a sound, scientifically based on managing of wildlands and also to assess likely implications due to changes in fire regime under a global change scenario. Few references can be found about fire ecology of Pinus uncinata Ram. (PU. PU species grows in the Central Pyrenees where large, severe wildland fires did not occur frequently in the past. However, several fires with extreme fire behavior have affected PU stands in last years and they might disturb other PU forest in the future.Area of study: Cabdella fire (February 2012, in Lleida province, is one of the several wildland fires occurred in 2012 (winter season in the Central Pyrenees. Fire affected a large PU plantation (102 ha located at 1.800-2,100 meters above the sea.Material and methods: We have analyzed first order fire effects in three fireline intensity thresholds along three years in terms of mortality ratio, scorched height, percentage of scorched crown volume and bark char height.Main results: PU seems to be a very tolerant species to low and medium fire line intensity but fire effects were very significant when fire line intensity was high. In medium fireline intensity sites, probability of mortality ranged from 15 to 30% and the dead trees had the highest values on scorched height and percentage of scorched crown volume.Research highlights: Results from this work supports that prescribed burning might be used to efficiently decrease fuel load and fuel vertical continuity while avoiding considerable PU mortality. It also displayed that when fuel management has been implemented, PU mortality might be limited even under extreme fire behavior.Abbreviations used: PU: Pinus uncinata Ram.

  13. Volatile constituents of Pinus roxburghii from Nepal

    Directory of Open Access Journals (Sweden)

    Prabodh Satyal

    2013-01-01

    Full Text Available Background: Pinus roxburghii Sarg. Is one of 3 species of pine found in Nepal, the oil of which is traditionally used to treat cuts, wounds, boils, and blisters. Objective: To obtain, analyze, and examine the anti-microbial and cytotoxic activities of the essential oils of P. roxburghii. Materials and Methods: Three plant parts (cone, needle, and bark of Pinus roxburghii were collected in Biratnagar, Nepal. The essential oils were obtained by hydrodistillation, and the chemical compositions were determined by GC-MS. The needle and cone essential oils were screened for anti-microbial activity against Bacillus cereus, Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli, and Aspergillus niger; brine shrimp (Artemia salina lethality; and in-vitro cytotoxicity against MCF-7 cells. Results: GC-MS analysis for the cone oil revealed 81 compounds with 78 components being identified (95.5% of the oil while 98.3% of needle oil was identified to contain 68 components and 98.6% of the bark oil (38 components was identified. The 3 essential oils were dominated by sesquiterpenes, particularly (E-caryophyllene (26.8%-34.5% and α-humulene (5.0%-7.3% as well as monoterpene alcohols terpinen-4-ol (4.1%-30.1% and α-terpineol(2.8%-5.0%. The monoterpene δ-3-carene was present only in needle and cone essential oils (2.3% and 6.8%, respectively. Bio-activity assays of the cone essential oil of P. roxburghii showed remarkable cytotoxic activity (100% killing of MCF-7 cells at 100 μg/mL along with notable brine shrimp lethality (LC50 =11.8 μg/mL. The cone essential oil did not show anti-bacterial activity, but it did exhibit anti-fungal activity against Aspergillus niger (MIC=39 μg/mL. Conclusion: The bioactivity of P. roxburghii essential oil is consistent with its traditional medicinal use.

  14. Book review / reseña: LORENTE, J. P., SÁNCHEZ GIMÉNEZ, S. y CABAÑAS BRAVO, M., Vae victis! Los artistas del exilio y sus museos.

    Directory of Open Access Journals (Sweden)

    Jesús F. Pascual Molina

    2010-01-01

    Full Text Available Reseña del libro: LORENTE, J. P., SÁNCHEZ GIMÉNEZ, S. y CABAÑAS BRAVO, M., Vae victis! Los artistas del exilio y sus museos, Gijón, Ediciones Trea, 2009. 340 páginas ISBN: 978-84-9704-458-5.

  15. Some soil properties and microbial biomass of Pinus maritima, Pinus pinea and Eucalyptus camaldulensis from the Eastern Mediterranean coasts

    OpenAIRE

    Nacide Kizildag; Husniye Aka Sagliker; Ahu Kutlay; Sahin Cenkseven; Cengiz Darici

    2012-01-01

    Background: Salt-affected soils occupy wide areas that have ecological importance in semi-arid and arid regions. Excessive amounts of salt have adverse effects on soil physical and chemical properties and also on the microbiological processes. The soils of Pinus maritima, Pinus pinea, and Eucalyptus camaldulensis were found to be under salinity stress in the present study area. Thus, the carbon, nitrogen, phosphorus contents, microbial biomass, and carbon mineralization were determined in the...

  16. Valores energéticos e composição bromatológica dos fenos de jureminha, feijão bravo e maniçoba para aves Energy values and chemical composition of jureminha, bravo beans and maniçoba hays for poultry

    Directory of Open Access Journals (Sweden)

    Fernando Guilherme Perazzo Costa

    2007-08-01

    Full Text Available Um experimento foi conduzido para determinação dos valores de energia metabolizável aparente (EMA e energia metabolizável aparente corrigida pelo balanço de nitrogênio (EMAn e da composição química dos fenos de feijão-bravo (FFB, jureminha (FJ e maniçoba (FM para uso na alimentação de aves. Duzentos e oitenta pintos com 17 dias de idade foram distribuídos aleatoriamente em sete tratamentos, em esquema fatorial 3 x 2 + 1 (três tipos de feno x dois níveis de substituição da dieta-referência + dieta-referência, com quatro repetições de dez aves. Os níveis de EMA e EMAn dos fenos de jureminha, feijão-bravo e maniçoba foram 3.205 e 2.911, 2.990 e 2.648, 2.728 e 2.419, determinados com 15% de substituição da dieta-referência pelo alimento e 2.678 e 2.371, 2.875 e 2.523, 2.277 e 1.956 com substituição de 30%, respectivamente, e evidenciaram influência do nível de substituição da dieta-referência sobre os valores de EMA e EMAn das dietas. Os valores de EMA e EMAn, para aves, dos fenos de jureminha e feijão-bravo são superiores aos do feno de maniçoba.An experiment was carried out to determine the apparent metabolizable energy (AME, apparent metabolizable energy corrected for nitrogen balance (AMEn and chemical composition of bravo bean hay (HBB, jureminha hay (HJ and maniçoba hay (HM for use in birds feeding. Two hundred and eighty, 17 days old broiler chicks were distributed according to a completely randomized design, into seven treatments organized in a factorial arrangement 3 x 2 + 1 (three hay types x two levels of substitution of the reference diet + reference diet, with four repetitions of 10 birds each. The contents of AME and AMEn of jureminha, bravo bean and maniçoba hays were 3.205 and 2.911, 2.990 and 2.648, 2.728 and 2.419, respectively, when the ingredients test substituted 15% of the reference diet, and 2.678 and 2.371, 2.875 and 2.523, 2.277 and 1.956, respectively, when the ingredients test

  17. Pinus monophylla establishment in an expanding Pinus-Juniperus woodland: Environmental conditions, facilitation and interacting factors

    Energy Technology Data Exchange (ETDEWEB)

    Chambers, Jeanne C. [USDA Forest Service, Reno, NV (United States). Rocky Mountain Research Station

    2001-02-01

    The tree species comprising Pinus-Juniperus woodlands are rapidly expanding into shrub-grasslands throughout their range. Observational studies indicate that establishment is facilitated by nurse plants, but little information exists on the mechanisms involved. I examined both abiotic and biotic factors influencing Pinus monophylla establishment in Artemisia tridentata steppe with expanding populations of P. monophylla and Juniperus osteosperma. I also examined the effects of seed burial and predation on seedling establishment. Microhabitats under trees and shrubs had higher extractable P and K, higher organic matter, total nitrogen and cation exchange capacity than interspace microhabitats. Soil water contents (0-15 cm) were lower in interspaces than under shrubs or trees due to dry surface (0-5 cm) soils. Soil temperatures (at 1 and 15 cm) were lowest under trees, intermediate under shrubs, and highest in interspaces. Timing and rate of seedling emergence were temperature dependent with the order of emergence paralleling mean growing season temperatures: tree interspace = shrub interspace > under shrub > under Juniperus {>=} under Pinus. Seed burial was required for rooting and the highest emergence occurred from depths of 1 and 3 cm indicating that caching by birds and rodents is essential and that animals bury seeds at adequate if not optimal depths for emergence. Seedlings required micro-environmental modification for survival; all seedlings, including those that emerged from seeds and transplants, died within the first year in interspace microhabitats. Survival in under-tree or under-shrub microhabitats depended on soil water availability and corresponded closely to soil water contents over the 3-yr study. Under-shrub microhabitats had more favourable soil and micro-environmental characteristics than under-tree microhabitats and had the highest seedling life spans for the first-year seedling cohort. Predation of Pinus seedlings by rodents was a significant

  18. by Pinus virginiana in Mine Restoration

    Directory of Open Access Journals (Sweden)

    Jenise M. Bauman

    2012-01-01

    Full Text Available This study evaluated the influence of planting sites on the establishment and ectomycorrhizal (ECM colonization of American chestnut (Castanea denetata (Marsh. Borkh. on an abandoned coal mine in an Appalachian region of the United States. Root morphotyping and sequencing of the fungal internal transcribed spacer (ITS region were used to identify the ECM species associated with the chestnut seedlings. Germination, survival, ECM root colonization, and growth were assessed in three habitats: forest edge, center (plots without vegetation, and pine plots (a 10-year-old planting of Pinus virginiana. Seedlings in pine plots had higher survival (38% than the other plot types (center 9% and forest edge 5%; P=0.007. Chestnuts found colonized by ECM within the pine plots were larger (P=0.02, contributed by a larger root system (P=0.03. Forest edge and pine plots had more ECM roots than seedlings in center plots (P=0.04. ITS fungal sequences and morphotypes found among chestnut and pine matched Scleroderma, Thelephora, and Pisolithus suggesting these two plant species shared ECM symbionts. Results indicated that the presence of P. virginiana had a greater facilitative effect on growth and survival of chestnut seedlings.

  19. Déterminisme génétique de la croissance en hauteur du Pin maritime (Pinus pinaster Ait.) . III. - Evolution des composants de la variance phénotypique et génotypique

    OpenAIRE

    Kremer, Antoine

    1981-01-01

    Cette étude se propose de suivre l’évolution des composantes de la variance phénotypique et génotypique de l’accroissement en hauteur en utilisant le coefficient de variation exprimé au niveau de chaque composante, l’accroissement en hauteur étant estimé par le cumul des accroissements successifs. Le coefficient de variation de l’erreur, de l’effet station, de l'interaction famille X station et de l’interaction mère X père décroît avec le temps alors que la variance des effets princip...

  20. Paired comparison of water, energy and carbon exchanges over two young maritime pine stands (Pinus pinaster Ait.): effects of thinning and weeding in the early stage of tree growth.

    Science.gov (United States)

    Moreaux, Virginie; Lamaud, Eric; Bosc, Alexandre; Bonnefond, Jean-Marc; Medlyn, Belinda E; Loustau, Denis

    2011-09-01

    The effects of management practices on energy, water and carbon exchanges were investigated in a young pine plantation in south-west France. In 2009-10, carbon dioxide (CO(2)), H(2)O and heat fluxes were monitored using the eddy covariance and sap flow techniques in a control plot (C) with a developed gorse layer, and an adjacent plot that was mechanically weeded and thinned (W). Despite large differences in the total leaf area index and canopy structure, the annual net radiation absorbed was only 4% lower in plot W. We showed that higher albedo in this plot was offset by lower emitted long-wave radiation. Annual evapotranspiration (ET) from plot W was 15% lower, due to lower rainfall interception and transpiration by the tree canopy, partly counterbalanced by the larger evaporation from both soil and regrowing weedy vegetation. The drainage belowground from plot W was larger by 113 mm annually. The seasonal variability of ET was driven by the dynamics of the soil and weed layers, which was more severely affected by drought in plot C. Conversely, the temporal changes in pine transpiration and stem diameter growth were synchronous between sites despite higher soil water content in the weeded plot. At the annual scale, both plots were carbon sinks, but thinning and weeding reduced the carbon uptake by 73%: annual carbon uptake was 243 and 65 g C m(-2) on plots C and W, respectively. Summer drought dramatically impacted the net ecosystem exchange: plot C became a carbon source as the gross primary production (GPP) severely decreased. However, plot W remained a carbon sink during drought, as a result of decreases in both GPP and ecosystem respiration (R(E)). In winter, both plots were carbon sources, plots C and W emitting 67.5 and 32.4 g C m(-2), respectively. Overall, this study highlighted the significant contribution of the gorse layer to mass and energy exchange in young pine plantations.

  1. Mycorrhizal association of maritime pine, Pinus pinaster, with Rhizopogon roseolus has contrasting effects on the uptake from soil and root-to-shoot transfer of {sup 137}Cs, {sup 85}Sr and {sup 95m}Tc

    Energy Technology Data Exchange (ETDEWEB)

    Ladeyn, Ingrid; Plassard, Claude [INRA, UMR 1222, Biogeochimie du Sol et de la Rhizosphere, place Viala, 34060 Montpellier (France); Staunton, Siobhan [INRA, UMR 1222, Biogeochimie du Sol et de la Rhizosphere, place Viala, 34060 Montpellier (France)], E-mail: staunton@montpellier.inra.fr

    2008-05-15

    The beneficial role of mycorrhizal association on plant nutrition and water supply is well-known, however, very little information exists with respect to the availability of radionuclides. We have measured the effect of controlled mycorrhizal association on the root uptake from soil and accumulation in leaves of three radionuclides. The radionuclides have contrasting chemical and biological properties: Cs is strongly adsorbed on soil, has no biological role and is a close analogue of potassium; Sr is less strongly adsorbed on soil and behaves very similarly to calcium; and Tc is very mobile in soil as pertechnetate, but immobilised when reduced to Tc(IV), it is also considered to be easily assimilated by biological systems. We found that mycorrhizal association had no effect on root-to-needle transfer of Cs, but increased root uptake and that this increase could not be explained by improved potassium nutrition. In contrast, the symbiotic relation decreased Tc soil-to-needle transfer, but this resulted from complex dynamics of root uptake and rapid immobilisation of Tc in soil. No effect of mycorrhizal association on Sr, like its stable analogue Ca, was observed. The addition of a phytotoxic metal, Cu, inhibited mycorrhizal association, thus eliminating the effects observed for non-contaminated plant-fungus couples, but had no additional effect on radionuclide dynamics.

  2. El valor del suelo habitacional y la intervención de agentes externos Valle de Bravo, Estado de México

    Directory of Open Access Journals (Sweden)

    Ariadna Zuleica Torres Pulido

    2015-03-01

    Full Text Available Como parte fundamental de los estudios de planeación se encuentra el territorio, el suelo como componente esencial de la estructura urbana y sustento de las diferentes actividades realizadas por el hombre, cobra singular importancia al momento que el valor, el uso y los agentes que en ellos interviene se vuelven factores que impulsan o retraen el desarrollo de un lugar, trayendo como consecuencia segregaciones sociales. De ahí que en este trabajo se da a conocer, a partir del caso de Valle de Bravo, la influencia que ejercen los agentes externos en los cambios del valor de suelo habitacional, tomando como punto de partida la evolución del patrón de ocupación del suelo y las actividades económicas.

  3. EL VALOR DEL SUELO HABITACIONAL Y LA INTERVENCIÓN DE AGENTES EXTERNOS VALLE DE BRAVO, ESTADO DE MÉXICO

    Directory of Open Access Journals (Sweden)

    Ariadna Zuleica Torres Pulido

    2010-01-01

    Full Text Available Como parte fundamental de los estudios de planeación se encuentra el territorio, el suelo como componente esencial de la estructura urbana y sustento de las diferentes actividades realizadas por el hombre, cobra singular importancia al momento que el valor, el uso y los agentes que en ellos interviene se vuelven factores que impulsan o retraen el desarrollo de un lugar, trayendo como consecuencia segregaciones sociales. De ahí que en este trabajo se da a conocer, a partir del caso de Valle de Bravo, la infl uencia que ejercen los agentes externos en los cambios del valor de suelo habitacional, tomando como punto de partida la evolución del patrón de ocupación del suelo y las actividades económicas.

  4. Realidad o quimera de los futuros conflictos hídricos: el caso de la cuenca del Río Grande/Bravo

    OpenAIRE

    Nava, L.F.

    2016-01-01

    La cuenca binacional del Río Grande/Río Bravo (RGB) es una fuente esencial de agua dulce para más de 13 millones de personas que viven en el Sudoeste de los Estados Unidos y el norte de México. La frontera entre estos países es conocida por ser una de las más activas en el mundo en términos de intercambios de bienes, y una de las más ajenas a la movilidad de personas. La sección de la frontera que corre el RGB representa una serie de asimetrías de poder y dinámicas de la globalización. Tambié...

  5. Manuel et Lola Álvarez Bravo : le couple fondateur de la modernité photographique au Mexique

    OpenAIRE

    Aubague, Laurent

    2014-01-01

    L'essor de la modernité photographique au Mexique tient au travail que Manuel Álvarez Bravo et sa femme Lola Álvarez initièrent dès les années 1930 au Mexique. La production de ce couple surprend à la découverte des multiples échos existant entre l’œuvre de l'un et de l'autre. L'ensemble de leur création est aussi important comme manifestation d'une ouverture à toutes les inventions de l'art photographique aux quatre coins du monde. En cela, ils ont été à l'unisson avec les recherches esthéti...

  6. [Storage proteins from seeds of Pinus pinea L].

    Science.gov (United States)

    Nasri, Nizar; Triki, Saïda

    2007-05-01

    The Mediterranean stone pine Pinus pinea L. (gymnosperm, Pinaceae) is much appreciated for its seed production, widely used in food preparation in the Mediterranean Basin. Seeds contain 25% proteins on a dry-weight basis. Pinus pinea accumulate globulins as major storage proteins in seeds (75% of total storage proteins), composed of several subunits of 10 to 150 kDa, revealed by SDS-PAGE. The albumin fraction (15%) represents three subunits of 14, 24 and 46 kDa. Glutelins, the least soluble fraction, represents a small proportion (10%). Their constitutive units have frequent PM of 43 kDa. Prolamins also represent a very small percentage (1 to 2%).

  7. Spontaneous Hybridization between Pinus mugo and Pinus sylvestris at the Lithuanian Seaside: A Morphological Survey

    Directory of Open Access Journals (Sweden)

    Darius Danusevičius

    2012-01-01

    Full Text Available We address the problem of spontaneous hybridization between an exotic species Pinus mugo and the native/local P. sylvestris at the seaside spit of Kursiu Nerija in Lithuania. The objective was to identify spontaneous hybrids between P. mugo and P. sylvestris based on morphology traits among the individuals naturally regenerating at the seaside spit. The field inventory was carried out over the entire Lithuanian part of the spit, and 200 individuals morphologically intermediate between P. sylvestris and P. mugo were identified. Based on a weighted trait index, the intermediate individuals were grouped into two groups, one morphologically close to P. sylvestris and another close to P. mugo. The needle micromorphological traits of the putative hybrids were of intermediate values between P. mugo and P. sylvestris. The results provide a strong evidence of spontaneous hybridization between P. mugo and P. sylvestris in Lithuanian seaside spit of Kursiu Nerija.

  8. Spontaneous hybridization between Pinus mugo and Pinus sylvestris at the Lithuanian seaside: a morphological survey.

    Science.gov (United States)

    Danusevičius, Darius; Marozas, Vitas; Brazaitis, Gediminas; Petrokas, Raimundas; Christensen, Knud Ib

    2012-01-01

    We address the problem of spontaneous hybridization between an exotic species Pinus mugo and the native/local P. sylvestris at the seaside spit of Kursiu Nerija in Lithuania. The objective was to identify spontaneous hybrids between P. mugo and P. sylvestris based on morphology traits among the individuals naturally regenerating at the seaside spit. The field inventory was carried out over the entire Lithuanian part of the spit, and 200 individuals morphologically intermediate between P. sylvestris and P. mugo were identified. Based on a weighted trait index, the intermediate individuals were grouped into two groups, one morphologically close to P. sylvestris and another close to P. mugo. The needle micromorphological traits of the putative hybrids were of intermediate values between P. mugo and P. sylvestris. The results provide a strong evidence of spontaneous hybridization between P. mugo and P. sylvestris in Lithuanian seaside spit of Kursiu Nerija.

  9. Evolution of genome size and complexity in Pinus.

    Directory of Open Access Journals (Sweden)

    Alison M Morse

    Full Text Available BACKGROUND: Genome evolution in the gymnosperm lineage of seed plants has given rise to many of the most complex and largest plant genomes, however the elements involved are poorly understood. METHODOLOGY/PRINCIPAL FINDINGS: Gymny is a previously undescribed retrotransposon family in Pinus that is related to Athila elements in Arabidopsis. Gymny elements are dispersed throughout the modern Pinus genome and occupy a physical space at least the size of the Arabidopsis thaliana genome. In contrast to previously described retroelements in Pinus, the Gymny family was amplified or introduced after the divergence of pine and spruce (Picea. If retrotransposon expansions are responsible for genome size differences within the Pinaceae, as they are in angiosperms, then they have yet to be identified. In contrast, molecular divergence of Gymny retrotransposons together with other families of retrotransposons can account for the large genome complexity of pines along with protein-coding genic DNA, as revealed by massively parallel DNA sequence analysis of Cot fractionated genomic DNA. CONCLUSIONS/SIGNIFICANCE: Most of the enormous genome complexity of pines can be explained by divergence of retrotransposons, however the elements responsible for genome size variation are yet to be identified. Genomic resources for Pinus including those reported here should assist in further defining whether and how the roles of retrotransposons differ in the evolution of angiosperm and gymnosperm genomes.

  10. Pathways of stand development in ageing Pinus sylvestris forests

    NARCIS (Netherlands)

    Kint, Vincent; Mohren, G.M.J.; Geudens, Guy; Wulf, de R.; Lust, Noel

    2004-01-01

    Question: What are the main pathways of long-term stand development in forest ecosystems on oligotrophic and acidic sandy soils? Location: Nine forest reserves at different locations in The Netherlands: all ageing Pinus sylvestris forests that are no longer managed and where massive regeneration of

  11. An interesting chemical polymorphism in Pinus sylvestris L.

    Directory of Open Access Journals (Sweden)

    Jerzy Szweykowski

    2014-01-01

    Full Text Available Intra- and interpopulational polymorphism in the production of phenolic compounds is described in Polish populations of Pinus sylvestris L. Two mutually exclusive forms of pine trees are present in changing proportions in all populations studied. This allows three groups of populations to be distinguished. The character of this differentiation is discussed.

  12. A new C-methylated flavonoid glycoside from Pinus densiflora.

    Science.gov (United States)

    Jung, M J; Choi, J H; Chung, H Y; Jung, J H; Choi, J S

    2001-12-01

    A new C-methyl flavonol glycoside, 5,7,8,4'-tetrahydroxy-3-methoxy-6-methylflavone 8-O-beta-D-glucopyranoside (1), has been isolated from the needles of Pinus densiflora, together with kaempferol 3-O-beta-(6"-acetyl)-galactopyranoside.

  13. [Systemic allergic reaction after ingestion of pine nuts, Pinus pinea].

    Science.gov (United States)

    Nielsen, N H

    1990-11-26

    An in vivo open oral provocation with pine nuts (Pinus pinea) confirmed information about systemic reaction after ingestion of pine nuts. In vitro tests suggested a systemic IgE allergic reaction. Pine nuts are employed in sweets and cakes and, as in the present case, in green salads.

  14. Variations in electrical impedance and phase angle among seedlings of Pinus densata and parental species in Pinus tabuliformis habitat environment

    Institute of Scientific and Technical Information of China (English)

    Xinyu Chen; Huwei Yuan; Xiange Hu; Jingxiang Meng; Xianqing Zhou; Xiao-Ru Wang; Yue Li

    2015-01-01

    Electrical impedance (EI) and phase angle (PHI) parameters in AC impedance spectroscopy are important electrical parameters in the study of medical pathology. However, little is known about their application in variation and genetic relationship studies of forest trees. In order to test whether impedance parameters could be used in genetic relationship analysis among conifer species, EI and PHI were measured in a seedling experiment test composed of Pinus tabuliformis, Pinus yunnanensis, and Pinus densata in a habitat of Pinus tabuliformis. The results showed that variations in both EI and PHI among species were sig-nificant in different electric frequencies, and the EI and PHI values measured in the two populations of P. densata were between the two parental species, P. yunnanensis and P. tabuliformis. These results show that these two impedance parameters could reflect the genetic relationship among pine species. This was the first time using the two AC impedance spectroscopy parameters to test the genetic relationship analysis between tree species, and would be a hopeful novel reference methodology for future studies in evolution and genetic variation of tree species.

  15. A Physical Assessment of the Opportunities for Improved Management of the Water Resources of the Bi-National Rio Grande/Rio Bravo Basin

    Science.gov (United States)

    Aparicio, J.; McKinney, D.; Valdes, J.; Guitron, A.; Thomas, G.

    2007-05-01

    The hydro-physical opportunities for expanding the beneficial uses of the fixed water supply in the Rio Grande/Bravo Basin to better satisfy an array of water management goals are examined. These include making agriculture more resilient to periodic conditions of drought, improving the reliability of supplies to cities and towns, and restoring lost environmental functions in the river system. This is a comprehensive, outcome-neutral, model- based planning exercise performed by some 20 technical, primarily non-governmental institutions from both countries, aimed at proposing strategies that can reduce future conflicts over water throughout the entire basin. The second track consists in generating a set of future water management scenarios that respond to the needs and objectives of the basin stakeholders in each segment and each country. An array of scenarios for improved water management has been developed for the lower Rio Grande/Rio Bravo basin in Texas and the Mexican state of Tamaulipas. Another set under development will focus on the Rio Conchos and the El Paso/Juarez region. Eventually, scenarios will be generated such that will comprehend the entire basin on both sides of the border. These scenarios are the product of consultations with agricultural water districts, governmental organizations and environmental NGOs. They include strategies for reducing the physical losses of water in the system, conservation transfers, improvements in the operations of the Mexican and international reservoirs, improvements in environmental flow conditions, improvements in reliability of water supplies, and drought coping strategies.These scenarios will be evaluated for hydrologic feasibility by the basin-wide model and the gaming exercises. Modeling is necessary to understand how these options will affect the entire system and how they can be crafted to maximize the benefits and avoid unintended or uncompensated effects. The scenarios that have the potential to provide large

  16. Protetores físicos para semeadura direta de Pinus elliottii Engelm Shelters for direct seeding of Pinus elliottii Engelm

    Directory of Open Access Journals (Sweden)

    Vilmar Luciano Matteiq

    2001-10-01

    Full Text Available A utilização de protetores físicos tem se revelado uma eficiente técnica para o sucesso da semeadura direta de pinus. O presente trabalho avaliou diferentes tipos e tamanhos de protetores físicos de pontos de semeadura para a implantação de povoamentos de Pinus elliottii por semeadura direta. Como protetores, foram utilizados copos plástico, copos de papel e laminado de madeira. Esses protetores foram colocados sobre os pontos semeados com três sementes cada. Foi avaliada a emergência, sobrevivência, perdas por pássaros, perdas por tombamento e número de pontos com, pelo menos, uma planta viva, seis meses após a semeadura. Os protetores utilizados influenciaram positivamente, nos resultados, para todas as variáveis analisadas, por evitar perdas de sementes causadas por arraste ou soterramento e, principalmente por diminuíram os danos causados por pássaros que juntamente com as formigas, foram os principais problemas encontrados na implantação do povoamento de pinus por semeadura direta.The use of individual shelters has become an efficient method for direct seeding of pine. This work investigated the use of different kinds and sizes of shelters placed at sowing sites to establish new populations of directly seeded Pinus elliottii. Plastic and paper cups with open bottoms, or wood slats were used as protective shields. These were placed at sowing sites, each one containing 3 seeds. Emergencies, survival, losses due to birds or lodging, and number of sites with at least one live plant were analyzed six months after sowing. Shelters had positive effects on all variables analyzed both for avoiding losses resulted from dragging or burying of seeds, and for diminidhing the harm caused by birds which along with ants were the main problems found during the establishment of directly seeded pinus population.

  17. Resistência de amendoim - bravo aos herbicidas inibidores da enzima ALS Resistance of the weed wild poinsettia to ALS inhibitor herbicides

    Directory of Open Access Journals (Sweden)

    Dionisio L. P. Gazziero

    1998-12-01

    Full Text Available Uma das práticas agrícolas mais eficientes para o controle de plantas daninhas na agricultura é o uso de herbicidas seletivos. Todavia, a aplicação de um mesmo herbicida, ou de herbicidas com o mesmo mecanismo de ação, durante anos consecutivos, na mesma área, pode resultar na seleção de biótipos de plantas daninhas resistentes a esses herbicidas. Com o objetivo de confirmar a resistência de um biótipo (E-298 da planta daninha amendoim-bravo (Euphorbia heterophylla aos herbicidas inibidores da enzima ALS, encontrado em área de cultivo de soja, no município de Cafelândia, PR, foi conduzido um experimento em área pertencente à Embrapa Soja, Londrina, PR. As plantas provenientes da população com suspeita de resistência foram tratadas com diversos herbicidas e doses, e comparadas com plantas de uma população suscetível de amendoim-bravo. Os tratamentos foram estabelecidos considerando as doses recomendadas dos herbicidas, metade delas e, duas, quatro e oito vezes superiores a recomendada. Os produtos e as doses aplicadas foram cloransulan nas doses 0,0; 0,015; 0,03;0,06; 0,12; 0,24 kg i.a./ha, mais o espalhante adesivo Agral 0,2% v/v, imazetaphapyr nas doses 0,0; 0,05; 0,1; 0,2; 0,4 e 0,8 kg i.a./ha, imazaquin nas doses 0,0; 0,075; 0,15; 0,3; 0,6; 1,2 kg i.a./ha, mais o espalhante adesivo Assist 0,3% v/v, sulfentrazone nas doses 0,0; 0,3; 0,6; 1,2; 2,4; 4,8 kg i.a./ha, mais o espalhante adesivo Assist 0,3% v/v, e lactofen nas doses 0,0; 0,082; 0,165; 0,33; 0,664; 1,329 kg i.a./ha. O biótipo resistente apresentou diferentes níveis de resistência cruzada entre os herbicidas cloransulan, imazethapyr e o imazaquin, e as relações médias de GR50 obtidas foram > 6,85; 11,90 e > 7,18, respectivamente. As curvas de dose-resposta do biótipo resistente apresentaram valores inferiores ao biótipo suscetível, em todas as doses estudadas para os herbicidas cloransulan, imazethapyr e imazaquin. Para os herbicidas sulfentrazone e

  18. Five year report on the medical follow up of Marshallese receiving special medical care related to 1954 Bravo fallout radiation (January 1992--1996)

    Energy Technology Data Exchange (ETDEWEB)

    Vaswani, A.N.; Howard, J.E.

    1999-06-01

    This is the 17th and final report of the Marshall Islands Medical Program as carried out by the Brookhaven National Laboratory (BNL). The purpose of these publications has been to provide information on the medical status of 253 Marshallese exposed to radiation fallout in 1954. The medical program fulfills a commitment to disclose unique medical information relevant to public health. Details of the Bravo thermonuclear accident that caused the exposure have been published. A 1955 article in the Journal of the American Medical Association, which described the acute medical effects on the population that required special medical care, remains a definitive and relevant description of events. Marshallese participation in this Congressionally mandated program is voluntary. Throughout the 44 years of the program, each participating individual`s relevant medical findings, laboratory data, disease morbidity, and mortality have been published in the BNL reports in a manner preserving patient confidentiality. In each report, there has been an attempt to interpret these findings and to infer the role of radiation exposure in their development. An equally important aspect of the reports has been the presentation of data that allows for analyses of the medical consequences of the Marshallese exposure.

  19. Vigilando la Calidad del Agua de los Grandes Rios de la Nacion: El Programa NASQAN del Rio Grande (Rio Bravo del Norte)

    Science.gov (United States)

    Lurry, Dee L.; Reutter, David C.; Wells, Frank C.; Rivera, M.C.; Munoz, A.

    1998-01-01

    La Oficina del Estudio Geologico de los Estados Unidos (U.S. Geological Survey, 0 USGS) ha monitoreado la calidad del agua de la cuenca del Rio Grande (Rio Bravo del Norte) desde 1995 como parte de la rediseiiada Red Nacional para Contabilizar la Calidad del Agua de los Rios (National Stream Quality Accounting Network, o NASOAN) (Hooper and others, 1997). EI programa NASOAN fue diseiiado para caracterizar las concentraciones y el transporte de sedimento y constituyentes quimicos seleccionados, encontrados en los grandes rios de los Estados Unidos - incluyendo el Misisipi, el Colorado y el Columbia, ademas del Rio Grande. En estas cuatro cuencas, el USGS opera actualmente (1998) una red de 40 puntos de muestreo pertenecientes a NASOAN, con un enfasis en cuantificar el flujo en masa (la cantidad de material que pasa por la estacion, expresado en toneladas por dial para cada constituyente. Aplicacando un enfoque consistente, basado en la cuantificacion de flujos en la cuenca del Rio Grande, el programa NASOAN esta generando la informacion necesaria para identificar fuentes regionales de diversos contaminantes, incluyendo sustancias qui micas agricolas y trazas elementos en la cuenca. EI efecto de las grandes reservas en el Rio Grande se puede observar segun los flujos de constituyentes discurren a 10 largo del rio. EI analisis de los flujos de constituyentes a escala de la cuenca proveera los medios para evaluar la influencia de la actividad humana sobre las condiciones de calidad del agua del Rio Grande.

  20. TRYPANOCIDAL ACTIVE COMPOUNDS FROM SCOTTISH ABIES NOBILIS AND PINUS SYLVESTRIS

    Directory of Open Access Journals (Sweden)

    Veronique Seidel

    2013-02-01

    Full Text Available Three active compounds obtained from the aerial parts of Abies nobilis and Pinus sylvestris isolated by different chromatographic techniques. Their structures were identified by NMR (1H, 13C, COSY, HMBC spectroscopic and mass spectrometric data and established as catechin, dehydroabietic acid and Dihydroconiferyl alcohol. The isolated compounds were exhibited activity against blood stream form of parasite Trypanosoma brucei brucei (S 427.

  1. Uptake of trifluoroacetate by Pinus ponderosa via atmospheric pathway

    Science.gov (United States)

    Benesch, J. A.; Gustin, M. S.

    Trifluoroacetate (TFA, CF 3COO -), a break down product of hydro(chloro)-fluorocarbons (HFC/HCFCs), has been suggested to contribute to forest decline syndrome. To investigate the possible effects, Pinus ponderosa was exposed to TFA applied as mist (150 and 10,000 ng l -1) to foliar surfaces. Needles accumulated TFA as a function of concentration and time. However, no adverse physiological responses, as plant morphology, photosynthetic and conductance rates, were observed at the TFA concentrations used in this study.

  2. DEVELOPMENT OF PINUS SYLVESTRIS L. MALE GAMETOPHYTE IN URBAN ENVIRONMENT

    OpenAIRE

    L.P. Khlebova; O. V. Bychkova

    2016-01-01

    The peculiarities of the development of the male gametophyte of Pinus silvestris L. in the urban environment were studied. Quality of pollen was evaluated by laboratory experiments through germination at the nutrient medium and by histochemical staining to determine the presence of redox enzymes. It was shown that pollen viability is closely linked to climatic and weather conditions during the period of microsporogenesis and mass pollination. Atmospheric pollution affected the pollen signific...

  3. Two new triterpenoids from the roots of Pinus densiflora.

    Science.gov (United States)

    Otaka, Junnosuke; Komatsu, Masabumi; Miyazaki, Yasumasa; Futamura, Yushi; Osada, Hiroyuki

    2017-03-01

    Chemical investigation of the roots of Pinus densiflora led to the isolation of two new triterpenoids, (24S)-3β-methoxy-24,25-epoxy-lanost-9(11)-ene (1) and 29-acetoxy-3α-methoxyserrat-14-en-21α-ol (2), together with three known serratene-type triterpenoids (3-5) and four known diterpenoids (6-9). Their structures were determined by spectroscopic analyses.

  4. Cutin fluorescence in early embryos of Pinus and Tsuga

    OpenAIRE

    Ewa Szczuka; Irena Gielwanowska

    2014-01-01

    Embryos of Pinus nigra Arnold and Tsuga canadensis Carr. (Pinaceae) at different stages of development were dissected from fresh, unfixed seeds and examined in a fluorescence microscope with 400 nm excitation light. The embryos of the investigated species showed cutin fluorescence after auramine 0 staining. At first the fluorescing cutin layer was formed on the apical part of the embryo with a well developed secondary suspensor, then it extended over the lateral surface of the embryo; the sus...

  5. Glacial Refugium of Pinus pumila (Pall.) Regel in Northeastern Siberia

    Energy Technology Data Exchange (ETDEWEB)

    Shilo, N A; Lozhkin, A V; Anderson, P M; Brown, T A; Pakhomov, A Y; Solomatkina, T B

    2007-02-10

    One of the most glowing representatives of the Kolyma flora [1], ''Pinus pumila'' (Pall.) Regel (Japanese stone pine), is a typical shrub in larch forests of the northern Okhotsk region, basins of the Kolyma and Indigirka rivers, and high-shrub tundra of the Chukchi Peninsula. It also forms a pine belt in mountains above the forest boundary, which gives way to the grass-underbrush mountain tundra and bald mountains. In the southern Chukchi Peninsula, ''Pinus pumila'' along with ''Duschekia fruticosa'' (Rupr.) Pouzar and ''Betula middendorffii'' Trautv. et C. A. Mey form trailing forests transitional between tundra and taiga [2]. Pinus pumila pollen, usually predominating in subfossil spore-and-pollen spectra of northeastern Siberia, is found as single grains or a subordinate component (up 2-3%, rarely 10%) in spectra of lacustrine deposits formed during the last glacial stage (isotope stage 2) in the Preboreal and Boreal times of the Holocene. Sometimes, its content increases to 15-22% in spectra of lacustrine deposits synchronous to the last glacial stage near the northern coast of the Sea of Okhotsk [3], evidently indicating the proximity of Japanese stone pine thickets.

  6. Fumonisin production by Gibberella fujikuroi strains from Pinus species.

    Science.gov (United States)

    Mirete, S; Patiño, B; Vázquez, C; Jiménez, M; Hinojo, M J; Soldevilla, C; González-Jaén, M T

    2003-12-31

    Fumonisins are important mycotoxins basically produced by strains from the Gibberella fujikuroi species complex (with anamorphs in Fusarium genus) which contaminate food and feed products representing a risk to human and animal health. In this work, we report for the first time the fumonisin production of Fusarium moniliforme Sheldon strains associated to edible pine nuts of Pinus pinea. P. pinea is an important and widely distributed Pinus species in the Mediterranean area where their pine nuts are consumed raw or slightly processed in diverse food products. In this work, characterization and further identification of those strains were performed by polymerase chain reaction-restriction fragment length polymorphisms (PCR-RFLPs) of the intergenic spacer region of the rDNA (IGS) with the aid of the eight mating populations (A-H) described for G. fujikuroi species complex. The method was powerful to detect polymorphism, allowing discrimination between individuals and could be used to study the genetic relationships among them and within the G. fujikuroi species complex. Fusarium strains associated to Pinus radiata were also included in the present study. These strains did not produce fumonisins and showed no close relation with the strains isolated from P. pinea. The approach used in this work was rapid and proved to be efficient to assist identification and to characterize and analyse relatedness of new isolates within the G. fujikuroi species complex.

  7. Ectomycorrhizal diversity associated with Cedrus deodara and Pinus wallichiana in the Kashmir Himalaya, India.

    Science.gov (United States)

    Itoo, Zahoor Ahmad; Reshi, Zafar A

    2014-01-01

    The present study was undertaken to document the ectomycorrhizal diversity associated with the Cedrus deodara and Pinus wallichiana in the Kashmir Himalaya, India. The extensive field surveys carried out in the Kashmir Himalaya at five study sites resulted in the collection and identification of 76 potential ectomycorrhizal fungal species associated with the Cedrus deodara and Pinus wallichiana. Maximum 32 number of species were found associated with Pinus wallichiana, 19 with Cedrus deodara and 25 species were found growing in association with both the conifers. The present study reveals that Cedrus deodara and Pinus wallichiana in the Kashmir Himalaya, India harbour diverse ectomycorrhizal fungal species.

  8. Mineral Analysis of Pine Nuts (Pinus spp. Grown in New Zealand

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    Leo P. Vanhanen

    2013-04-01

    Full Text Available Mineral analysis of seven Pinus species grown in different regions of New Zealand; Armand pine (Pinus armandii Franch, Swiss stone pine (Pinus cembra L., Mexican pinyon (Pinus cembroides Zucc. var. bicolor Little, Coulter pine (Pinus coulteri D. Don, Johann’s pine (Pinus johannis M.F. Robert, Italian stone pine (Pinus pinea L. and Torrey pine (Pinus torreyana Parry ex Carrière, was carried out using an inductively coupled plasma optical emission spectrophotometer (ICP-OES analysis. Fourteen different minerals (Al, B, Ca, Cr, Cu, Fe, K, Mg, Mn, Na, Ni, P, S and Zn were identified in all seven varieties, except that no Al or Na was found in Pinus coulteri D. Don. New Zealand grown pine nuts are a good source of Cu, Mg, Mn, P and Zn, meeting or exceeding the recommended RDI for these minerals (based on an intake of 50 g nuts/day while they supplied between 39%–89% of the New Zealand RDI for Fe. Compared to other commonly eaten tree-nuts New Zealand grown pine nuts are an excellent source of essential minerals.

  9. Photosynthesis and respiration in the needles of Pinus sibirica and Pinus pumila and their putative hybrids

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    A.P. Zotikova

    2013-12-01

    Full Text Available A putative interspecific hybridization in Pinaceae family was investigated. Very rarely the physiological methods were involved in hybridization processes that occurs in the hybrid zones. It is well known that in most gymnosperms, the plastid genome is inherited from the paternal component while the mitochondrion is inherited from the maternal one. Therefore functioning pattern of organelles in the hybrid plant can suggest parent, from which they were inherited. The aim of this study was to indirectly establish the inheritance energy-transducing organelles (mitochondria, chloroplast according to their functioning. Current year needles from Siberian Stone Pine (Pinus sibirica Du Tour and Japanese Stone Pine (Pinus pumila (Pall. Regel as parent species and their putative hybrids were collected from Baikal Region. The photosynthesis rate was determined by using the spectrophotometer. The study of emission CO2 under dark respiration of needle was conducted with laser optical-acoustic gasanalyzer. The quantity was measured at 1, 2 and 3 hour after experiment start. The rate of the photoreduction ferricyanide potassium was characterized by the primary photochemical processes activity at the level of photosystem II. Comparison of pure species was shown that Japanese Stone Pine had higher functional activity of chloroplast as compared with SiberianStone Pine in spite of the fact that they are growing in similar environment conditions. Two of three analyzed hybrids had decreasedactivity of their chloroplasts. Unfortunately, in this case we can't conclude if the chloroplasts were inherited from Siberian Stone Pine orfrom Japanese Stone Pine. Chloroplast activity of the third hybrid was approximately similar to that of Japanese Stone Pine suggesting thatits chloroplasts were inherited from this parent. Consequently, the Siberian Stone Pine and the Japanese Stone Pine were maternal and paternal, respectively parents of

  10. Application of various statistical methods to analyze genetic diversity of Austrian (Pinus nigra Arn. and Scots pine (Pinus sylvestris L. based on protein markers

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    Lučić Aleksandar

    2011-01-01

    Full Text Available This paper presents the results of studies on protein polymorphism in seeds of Scots pine (Pinus sylvestris L. and Austrian pine (Pinus nigra Arn. as the most important economic species of the genus Pinus in Serbia. Polymorphism of protein markers was determined in selected genotypes originating from seven populations (Scots pine and six populations (Austrian pine. Analysis of protein markers was performed using two statistical methods, NTSYS and correspondence analysis. Both methods give the same arrangement of the analyzed populations, whereby, because of a different view of genetic distances, they can and should be combined, enabling easier and more precise understanding of mutual relationships of the observation units.

  11. El hombre y la mujer en la traducción. Análisis del comportamiento de la traducción de Carmen Martín Gaite frente a la de Juan Bravo Castillo en Madame Bovary

    OpenAIRE

    Sánchez Moros, Tamara

    2016-01-01

    Uno de los asuntos que se dirimen en la teoría de la traducción es la existencia o no de una supuesta traducción femenina. Sin pretender entrar en tal dilema, el presente trabajo de fin de grado analizará el comportamiento interno de las traducciones de una traductora y de un traductor: la de Carmen Martín Gaite y la de Juan Bravo Castillo sobre la novela de Gustave Flaubert, Madame Bovary. A través del reconocimiento de los diferentes procedimientos de traducción utilizados po...

  12. UNA EXPERIENCIA DE COOPERACIÓN PARA LA DEFINICIÓN DE ESTRATEGIAS DE CARA A LA PUESTA EN MARCHA DE PROYECTOS DE ECOTURISMO EN LA CUENCA DE VALLE DE BRAVO-AMANALCO (ESTADO DE MÉXICO)

    OpenAIRE

    Galacho Jiménez, Federico Benjamín; Arrebola Castaño, Juan Antonio; Blanco Sepúlveda, Rafael; Gómez Moreno, María Luisa; Luque Gil, Ana María; Rubio Barquero, Luís Miguel

    2012-01-01

    Este capítulo de libro esta basado en la experiencia desarrollada al amparo del proyecto A/023607/09, financiado por la AECID, que lleva por título: “Definición de estrategias para la puesta en marcha de proyectos de ecoturismo en la Cuenca de Valle de Bravo-Amanalco –Estado de México, México-". Se exponen las estratégicas y los mecanismos de actuación sobre las que asentar posibles alternativas de desarrollo local y rural basadas en proyectos de ecoturismo, siempre de acuerdo con el aprovech...

  13. LOCALIDADES CON RECURSOS TURÍSTICOS Y EL PROGRAMA PUEBLOS MÁGICOS EN MEDIO DEL PROCESO DE LA NUEVA RURALIDAD. LOS CASOS DE TEPOTZOTLÁN Y VALLE DE BRAVO EN EL ESTADO DE MÉXICO

    OpenAIRE

    Guadalupe Hoyos Castillo; Oscar Hernández Lara

    2008-01-01

    Este artículo examina el proceso socio territorial de los municipios de Tepotzotlán y Valle de Bravo, a propósito de la instrumentación del Programa Pueblos Mágicos, esto desde la perspectiva analítica de la nueva ruralidad de perfil turístico. Aquí sostenemos que el programa puede acelerar los cambios sociales y de actividades en el territorio, al tiempo que concentra inversión ya que focaliza su actuación en estructuras turísticas dinámicas con larga trayectoria sin alcanzar derrama territo...

  14. Factores bióticos y abióticos que determinan la seroprevalencia de anticuerpos contra Trypanosoma Cruzi en el municipio de Palmar de Bravo, Puebla, México Biotic and abiotic determinants of seroprevalence of antibodies against Trypanosoma cruzi in Palmar de Bravo, Puebla, Mexico

    Directory of Open Access Journals (Sweden)

    Francisca Sosa-Jurado

    2004-02-01

    Full Text Available OBJETIVO: Determinar la prevalencia de anticuerpos contra Trypanosoma cruzi y su relación con los factores bióticos y abióticos en Palmar de Bravo, Puebla, México. MATERIAL Y MÉTODOS: Estudio transversal efectuado en agosto de 2000 a septiembre de 2001, con una muestra aleatoria simple de 390 voluntarios residentes en Palmar de Bravo, Puebla, México. Se hizo determinación de anticuerpos contra T cruzi con técnicas serológicas validadas, búsqueda del vector y de reservorios domésticos, así como determinación de asociación entre caso positivo y factores de riesgo bióticos y abióticos. El análisis estadístico consistió en índice Kappa para las pruebas diagnósticas, empleando tabla de contingencia de 2 x 2; ji cuadrada corregida de Yates, exacta de Fisher y la razón de posibilidad para estimar la significancia de la asociación de factores bióticos y abióticos. RESULTADOS: La seroprevalencia fue de 4% en la población humana estudiada y de los reservorios (equinos, porcinos y caninos, sólo 10% de los caninos resultaron reactivos. Los vectores identificados fueron T barberi y T pallidipennis, con índice de dispersión e índice de colonización de 55 y 40%, respectivamente. Los factores de riesgo más importantes fueron la altitud (>2 150 y OBJECTIVE: To establish the relationship between seroprevalence for antibodies against Trypanosoma cruzi and its relationship with biotic and abiotic factors. MATERIAL AND METHODS: A cross-sectional study was conducted between August 2000 and September 2001. The study population consisted of a simple random sample of 390 volunteers residing in Palmar de Bravo, Puebla, Mexico. Sample and data collection procedures included assaying antibodies against T cruzi with validated assays, and searching for domestic reservoirs and triatomine bugs. The relationship between biotic and abiotic factors with seropositivity was assessed. Statistical analysis was conducted using Kappa values for

  15. Emerging Needle Blight Diseases in Atlantic Pinus Ecosystems of Spain

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    Esther Ortíz de Urbina

    2016-12-01

    Full Text Available Red band needle blight caused by Dothistroma septosporum and D. pini, and brown spot needle blight caused by Lecanosticta acicola provoke severe and premature defoliation in Pinus, and subsequent reduction of photosynthetic surfaces, vitality, and growth in young and adult trees. The recurrent damage results in branch and tree death. Until recently, pine needle blight diseases have had only minor impacts on native and exotic forest trees in the North of Spain, but in the past five years, these pathogen species have spread widely and caused severe defoliation and mortality in exotic and native plantations of Pinus in locations where they were not detected before. In an attempt to understand the main causes of this outbreak and to define the effectiveness of owners’ management strategies, four research actions were implemented: a survey of the management activities implemented by the owners to reduce disease impact, the evaluation of specific symptoms and damage associated with infection, and the identification of the causative pathogenic species and their reproductive capacity. Morphological characteristics of the fungus and molecular identification were consistent with those of Lecanosticta acicola and Dothistroma spp., D. septosporum, D. Pini, and both mating types were present for the three identified pathogens. The local silvicultural management performed, mainly pruning and thinning, was not resulting in the expected improvement. The results of this study can be applied to establish guidelines for monitoring and controlling the spread of needle blight pathogens.

  16. Annual shoot growth components related to growth of Pinus brutia.

    Science.gov (United States)

    Isik, Fikret; Isik, Kani; Yildirim, Tolga; Li, Bailian

    2002-01-01

    Shoot elongation patterns of Pinus brutia Ten. were studied in six natural populations and 10 open-pollinated families within each population. The data were collected from a provenance-progeny trial that was thinned at Ages 13 and 17 years. Annual height increment was partitioned into first flush (spring shoot) and subsequent flushes (summer shoots) and the contribution of each to annual height increment was measured from Ages 7 to 17. Spring shoot elongation patterns were similar in all populations and families for 9 out of 10 years. In contrast, at all ages, populations differed significantly in total summer shoot growth and number of summer flushes. Families within populations differed in number of summer flushes in 7 out of 10 years. Summer shoot growth was the major cause of the differences in annual height growth among the six populations. Significant and high correlations were observed between summer shoot growth at Ages 7 to 12 and height at Age 13. A population from near the middle of the species' altitudinal range had more summer flushes than populations from higher or lower elevations, indicating an opportunistic growth pattern. Compared with mid-elevation populations, low- and high-elevation populations had more conservative growth patterns that depended mainly on growth of spring shoots. We conclude that summer shoot growth can serve as an explanatory variable to predict height growth of populations. Differences in shoot elongation patterns among Pinus brutia populations may be useful for selecting seed sources and for gene conservation programs.

  17. Modelagem do crescimento e de biomassa individual de Pinus

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    Ana Beatriz Schikowski

    2013-09-01

    Full Text Available Este estudo tem como objetivo testar modelos matemáticos para estimativas de biomassa de diferentes compartimentos de Pinus spp., a partir de variáveis de fácil mensuração. Os dados utilizados são provenientes de plantios localizados no centro sul do estado do Paraná. Foram utilizados dados de peso seco total e parcial de 35 árvores de Pinus spp., obtidos por meio do método destrutivo direto. De cada árvore amostrada foram medidos também o CAP (circunferência à altura do peito e a altura total. Os modelos para estimativa de biomassa de folhagem não apresentaram bom desempenho, verificado pelos indicadores de ajuste. Entretanto, para os compartimentos: galhos, raízes, casca, fuste e para biomassa total, os ajustes apresentaram elevados valores de R² e baixos valores de Syx%. O modelo de crescimento de Richards obteve melhor desempenho que os demais testados para a estimativa da biomassa total.

  18. Factors Affecting Growth of Pinus radiata in Chile

    Science.gov (United States)

    Alvarez-Munoz, Jose Santos

    The Chilean forestry industry is based on hundreds of thousands of hectares of Pinus radiata plantations that have been established in a variety of soil and climate conditions. This approach has resulted in highly variable plantation productivity even when the best available technology was used. Little information is known about the ecophysiology basis for this variability. We explored the spatial and temporal variation of stand growth in Chile using a network of permanent sample plots from Modelo Nacional de Simulacion de Pino radiata. We hypothesized that the climate would play an important role in the annual variations in productivity. To answer these questions we developed the following projects: (1) Determination of site resource availability from historical data from automatic weather stations (rainfall, temperatures) and a geophysical model for solar irradiation, (2) Determination of peak annual leaf area index (LAI) for selected permanent sample plots using remote sensing technologies, (3) Analysis of soil, climate, canopy and stand factors affecting the Pinus radiata plantation growth and the use efficiency of site resources. For project 1, we estimated solar irradiation using the r.sun , Hargreaves-Samani (HS), and Bristow-Campbell (BC) models and validated model estimates with observations from weather stations. Estimations from a calibrated r.sun model accounted for 94% of the variance (r2=0.94) in monthly mean measured values. The r.sun model performed quite well for a wide range of Chilean conditions when compared with the HS and BC models. Our estimates of global irradiation may be improved with better estimates of cloudiness as they become available. Our model was able to provide spatial estimates of daily, weekly, monthly and yearly solar irradiation. For project 2, we estimated the inter-annual variation of LAI (Leaf Area Index), using remote sensing technologies. We determined LAI using Landsat Thematic Mapper (TM) data covering a 5 year period

  19. The design of a real-time formative evaluation of the implementation process of lifestyle interventions at two worksites using a 7-step strategy (BRAVO@Work

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    Wierenga Debbie

    2012-08-01

    Full Text Available Abstract Background Worksite health promotion programs (WHPPs offer an attractive opportunity to improve the lifestyle of employees. Nevertheless, broad scale and successful implementation of WHPPs in daily practice often fails. In the present study, called BRAVO@Work, a 7-step implementation strategy was used to develop, implement and embed a WHPP in two different worksites with a focus on multiple lifestyle interventions. This article describes the design and framework for the formative evaluation of this 7-step strategy under real-time conditions by an embedded scientist with the purpose to gain insight into whether this this 7-step strategy is a useful and effective implementation strategy. Furthermore, we aim to gain insight into factors that either facilitate or hamper the implementation process, the quality of the implemented lifestyle interventions and the degree of adoption, implementation and continuation of these interventions. Methods and design This study is a formative evaluation within two different worksites with an embedded scientist on site to continuously monitor the implementation process. Each worksite (i.e. a University of Applied Sciences and an Academic Hospital will assign a participating faculty or a department, to implement a WHPP focusing on lifestyle interventions using the 7-step strategy. The primary focus will be to describe the natural course of development, implementation and maintenance of a WHPP by studying [a] the use and adherence to the 7-step strategy, [b] barriers and facilitators that influence the natural course of adoption, implementation and maintenance, and [c] the implementation process of the lifestyle interventions. All data will be collected using qualitative (i.e. real-time monitoring and semi-structured interviews and quantitative methods (i.e. process evaluation questionnaires applying data triangulation. Except for the real-time monitoring, the data collection will take place at baseline and

  20. Antimicrobial activities of several parts of Pinus brutia, Juniperus oxycedrus, Abies cilicia, Cedrus libani and Pinus nigra.

    Science.gov (United States)

    Diğrak, M; Ilçim, A; Hakki Alma, M

    1999-11-01

    In this study, the antimicrobial activities of several parts of various trees grown in the Kahramanmaraş region of Turkey were investigated by the disc diffusion method. Chloroform, acetone and methanol extracts of leaves, resins, barks, cones and fruits of Pinus brutia Ten., Juniperus oxycedrus L., Abies cilicia Ant. & Kotschy Carr., Cedrus libani A. Rich. and Pinus nigra Arn. were prepared and tested against Bacillus megaterium DSM 32, Bacillus subtilis IMG 22, Bacillus cereus FMC 19, Escherichia coli DM, Klebsiella pneumoniae FMC 3, Enterobacter aerogenes CCM 2531, Staphylococcus aureus Cowan 1, Mycobacterium smegmatis RUT, Proteus vulgaris FMC 1, Listeria monocytogenes Scoot A, Pseudomonas aeruginosa DSM 5007, Candida albicans CCM 314, Candida tropicalis MDC 86 and Penicillium italicum K. The results showed that antifungal effects were not observed for the whole extracts, E. coli was not inhibited by any of the plant extracts except by the chloroform and acetone extracts of the leaves of A. cilicia, which showed inhibition zones of 16-18 mm, respectively. All the plant extracts used in this study inhibited the development of the other bacteria studied. When the results of this study were compared with an ampicillin standard, it was found that the microorganisms studied were generally susceptible, intermediate or resistant to the extracts of species when compared with the ampicillin standard. On the other hand, the acetone and methanol extracts of Juniperus fruits were found to be quite resistant.

  1. Some soil properties and microbial biomass of Pinus maritima, Pinus pinea and Eucalyptus camaldulensis from the Eastern Mediterranean coasts

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    Nacide Kizildag

    2012-12-01

    Full Text Available Background: Salt-affected soils occupy wide areas that have ecological importance in semi-arid and arid regions. Excessive amounts of salt have adverse effects on soil physical and chemical properties and also on the microbiological processes. The soils of Pinus maritima, Pinus pinea, and Eucalyptus camaldulensis were found to be under salinity stress in the present study area. Thus, the carbon, nitrogen, phosphorus contents, microbial biomass, and carbon mineralization were determined in the soils sampled from the Tarsus-Karabucak Forest of the Eastern Mediterranean Region (Turkey. Method: Carbon mineralization of all samples was measured by the CO2 respiration method over 30 d at 28�C and constant moisture. Results: There were no significant differences in the carbon mineralization among the soils. The average fungi count in 1 g of air dried soils of E. camaldulensis, P. pinea, and P. maritima were found to be a 72000 colony forming unit (cfu/g, 25300 cfu/g, and 28500 cfu/g, respectively. The total bacterial counts were 4x103 cfu/g, 10x103 cfu/g, and 7x103 cfu/g and the counts of anaerobic bacteria were 17800 cfu/g, 42900 cfu/g, and 27300 cfu/g, respectively. Conclusion: It is possible to conclude that salt, as an ecological factor, had no effect on microbial activity. This may be as a result of heavy rains which decreased the salt concentrations of the soil in the sampling region.

  2. Individual Growth Environment Suitable for Naturally Regenerated Young Pinus koraiensis under Secondary Natural Forest

    Institute of Scientific and Technical Information of China (English)

    FANShaohui; ZHANGQun; SHENHailong

    2004-01-01

    Impacts on growth of young trees of Pinus Koraiensis of 6 environmental factors of intensity of sunlight, direct sunlight, thickness of soil humus, neighboring trees, upper canopy species, herbs and shrubs were investigated on young tree of Pinus Koraiensis and 4 neighboring trees which are considered the structural unit of the microenvironment. Results indicated that the 6 environmental factors under investigation had effects, to various extents, on growth of the young trees. Based on the findings, suitable growing conditions for regenerated young tree of Pinus Koraiensis under forest were identified and corresponding silvicultural measures were proposed for operational practice.

  3. 火炬松、湿地松和马尾松采穗圃营建技术%Building Techniques of Cuttings Orchard of Pinus taeda,Pinus elliottii and Pinus massoniana

    Institute of Scientific and Technical Information of China (English)

    来端

    2001-01-01

    In this paper,the effect of time and retention hei ght of topping on quantity,length and basal diameter of coppice shoot of Pinus taeda,Pinus elliottii and Pinus massonian a has been studied.The results showed that because of difference of b iological properties among three tree species, suitable time and retention heigh t of topping among three tree species are different.The suitable topping time o f Pinus taeda is August 20 to August 25,the best retention height of topping is 15 cm; The suitable topping time of Pinus masson iana is July 30, the best retention height of topping is 8 cm; The su itable topping time of Pinus elliottii is August 15 to Aug ust 20,the best retention height of topping is 15 cm.%通过不同剪顶时间和剪顶高度对火炬松、湿地松和马尾 松的萌条数量、长度和径粗的影响研究.结果表明:不同树种由于其生物学特性不同,适宜 的截顶时间、截顶高度表现不一致.火炬松适宜的截顶时间为8月20日至8月25日,截顶高度 以15 cm为最宜;马尾松适宜的截顶时间为7月30日,截顶高度为8 cm;湿地松适宜的截顶时 间为8月15日至8月20日,截顶高度为15 cm.

  4. Quality of Pinus elliottii sawn timber from tapped forests

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    Pedro Henrique Gonzalez de Cademartori

    2012-12-01

    Full Text Available This study aimed to characterize the quality of Pinus elliottii sawn timber extracted from tapped forests and processed in a sawmill in São José do Norte (RS. Four butt logs and four upper logs for each of the three existing diameter grades were selected and sawed. The wood pieces were analyzed after sawing and after kiln drying. The presence of knots, which occurred due to the absence of forest management and influenced the qualitative classification of the wood pieces, was observed mainly in the samples from upper logs. The process of resin tapping contributed to a higher incidence of resin pockets in the samples from butt logs, also influencing the qualitative classification of the samples. The appearance of drying defects did not modify the classification of the wood samples from butt and upper logs.

  5. Needles of Pinus halepensis as biomonitors of bioaerosol emissions.

    Science.gov (United States)

    Galès, Amandine; Latrille, Eric; Wéry, Nathalie; Steyer, Jean-Philippe; Godon, Jean-Jacques

    2014-01-01

    We propose using the surface of pine trees needles to biomonitor the bioaerosol emissions at a composting plant. Measurements were based on 16S rRNA gene copy numbers of Saccharopolyspora rectivirgula, a bioindicator of composting plant emissions. A sampling plan was established based on 29 samples around the emission source. The abundance of 16S rRNA gene copies of S. rectivirgula per gram of Pinus halepensis needles varied from 104 to 102 as a function of the distance. The signal reached the background level at distances around the composting plant ranging from 2 km to more than 5.4 km, depending on the local topography and average wind directions. From these values, the impacted area around the source of bioaerosols was mapped.

  6. NUTRIENTS CONCENTRATION AND RETRANSLOCATION IN THE Pinus taeda L. NEEDLES

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    Márcio Viera

    2010-03-01

    Full Text Available Aiming at evaluating nutrients concentration and retranslocation in the Pinus taeda L. needles, this study was developed in two stands, in native grass area and in second rotation area, with same species and same age (7.5 years old in Cambará do Sul, RS. The needles were collected in plants in four orthogonal points (South, North, East and West, sampled new needles, mature needles and old needles. The material was dried in a stove, milled and chemically analyzed (macro and micronutrients. The concentrations of N, P, K, B, Cu and Zn had decreased, of Ca, Fe and Mn increased, and the Mg and S have remained constant with the age of the needles. The retranslocation rate (old-new needles was more than 50% for most nutrients, except for Mn and Fe, showed that cumulative effect and the Ca reference element.

  7. Variation among matsutake ectomycorrhizae in four clones of Pinus sylvestris.

    Science.gov (United States)

    Vaario, Lu-Min; Lu, Jinrong; Koistinen, Arto; Tervahauta, Arja; Aronen, Tuija

    2015-04-01

    Tricholoma matsutake is an ectomycorrhizal fungus that forms commercially important mushrooms in coniferous forests. In this study, we explored the ability of T. matsutake to form mycorrhizae with Pinus sylvestris by inoculating emblings produced through somatic embryogenesis (SE) in an aseptic culture system. Two months after inoculation, clones with less phenolic compounds in the tissue culture phase formed mycorrhizae with T. matsutake, while clones containing more phenols did not. Effects of inoculation on embling growth varied among clones; two of the four tested showed a significant increase in biomass and two had a significant increase in root density. In addition, results suggest that clones forming well-developed mycorrhizae absorbed more Al, Fe, Na, P, and Zn after 8 weeks of inoculation. This study illustrates the value of SE materials in experimental work concerning T. matsutake as well as the role played by phenolic compounds in host plant response to infection by mycorrhizal fungi.

  8. Isolation and characterization of Korean pine (Pinus koraiensis) convicilin.

    Science.gov (United States)

    Jin, Tengchuan; Wang, Yang; Chen, Yu-Wei; Albillos, Silvia M; Kothary, Mahendra H; Fu, Tong-Jen; Tankersley, Boyce; McHugh, Tara H; Zhang, Yu-Zhu

    2014-07-01

    A vicilin-like globulin seed storage protein, termed convicilin, was isolated for the first time from Korean pine (Pinus koraiensis). SDS-PAGE analysis revealed that Korean pine convicilin was post-translationally processed. The N-terminal peptide sequences of its components were determined. These peptides could be mapped to a protein translated from an embryo abundant transcript isolated in this study. Similar to vicilin, native convicilin appeared to be homotrimeric. Differential scanning calorimetry (DSC) analyses revealed that this protein is less resistant to thermal treatment than Korean pine vicilin. Its transition temperature was 75.57 °C compared with 84.13 °C for vicilin. The urea induced folding-unfolding equilibrium of pine convicilin monitored by intrinsic fluorescence could be interpreted in terms of a two-state model, with a Cm of 4.41 ± 0.15 M.

  9. Microwave vacuum drying characteristics of Pinus massoniana wood

    Institute of Scientific and Technical Information of China (English)

    Li Xian-jun; Li Wen-jun; Zhang Bi-guang

    2007-01-01

    Microwave-vacuum (MV) drying characteristics of plantation Masson pine (Pinus massoniana) were studied experimentally for various levels of microwave radiation time, initial moisture content (MC), vacuum level and wood thickness. The results show that the process of MV drying for wood can be significantly divided into a short accelerating rate drying period, a long constant rate drying period and falling rate drying period, and the second drying period can extend to levels of mean MC below the fiber saturation point. With the increase of initial MC and microwave radiation time, the drying rate of wood increases significantly. The vacuum level affects the drying rate in a slightly positive way. Within the range of 2 to 6 cm, the effects of sample thickness on the drying rate can be negligible.

  10. Needles of Pinus halepensis as biomonitors of bioaerosol emissions.

    Directory of Open Access Journals (Sweden)

    Amandine Galès

    Full Text Available We propose using the surface of pine trees needles to biomonitor the bioaerosol emissions at a composting plant. Measurements were based on 16S rRNA gene copy numbers of Saccharopolyspora rectivirgula, a bioindicator of composting plant emissions. A sampling plan was established based on 29 samples around the emission source. The abundance of 16S rRNA gene copies of S. rectivirgula per gram of Pinus halepensis needles varied from 104 to 102 as a function of the distance. The signal reached the background level at distances around the composting plant ranging from 2 km to more than 5.4 km, depending on the local topography and average wind directions. From these values, the impacted area around the source of bioaerosols was mapped.

  11. Germinación in vitro de Pinus cubensis Griseb

    Directory of Open Access Journals (Sweden)

    Raima Cantillo Ardebol

    2006-01-01

    Full Text Available Se hace alusión a la micropropagación de Pinus cubensis, Griseb, como aspecto que satisface la demanda de semillas para la reforestación en áreas de Pinares de Mayarí, hábitat de esta endémica región, amenazada por minería y explotación maderera. Se utilizaron semillas, determinando el mejor método de desinfección, evaluando su germinación en medio con diferentes composiciones de reguladores del crecimiento, y la influencia de la presencia o ausencia de la cubierta seminal. El mejor resultado se obtuvo con Cloralex al 20% durante 15 minutos e implantando semillas sin testa en medio MS libre de reguladores del crecimiento.

  12. Cutin fluorescence in early embryos of Pinus and Tsuga

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    Ewa Szczuka

    2014-01-01

    Full Text Available Embryos of Pinus nigra Arnold and Tsuga canadensis Carr. (Pinaceae at different stages of development were dissected from fresh, unfixed seeds and examined in a fluorescence microscope with 400 nm excitation light. The embryos of the investigated species showed cutin fluorescence after auramine 0 staining. At first the fluorescing cutin layer was formed on the apical part of the embryo with a well developed secondary suspensor, then it extended over the lateral surface of the embryo; the suspensor remained nonfluorescent. The fluorescing cutin layer occurred on the apical and side surface of the embryo, undergoing differentiation into the shoot axis and root initials. It is assumed that polarization and nutrition of the embryo may be influenced by presence of the cuticle.

  13. ESTABLISHMENT OF Pinus elliottii Engelm STANDS WITH DIRECT FIELD SOWING

    Directory of Open Access Journals (Sweden)

    César Augusto Guimarães Finger

    2010-08-01

    Full Text Available This experiment was undertaken to test the possibility of use of direct field sowing as a method of  forest formation. The soil preparation consisted of mechanic mowing followed by cleaning of small circles of 40 cm of diameter, where four seeds of Pinus elliottii Engelm were sowed, previously stratified, and protected by a bottomless plastic glass fastened to the sowing point. The treatments tested were direct sowing, sowing of seeds involved by paper envelop and seedling plantation as testify treatment. The results were not satisfactory, however, being observed at the most 38.46% of the sowing places with seedlings after 84 days of sowing. The main factor that contribuited to the failure of the method was seedling mortality caused by water deficit and high temperatures.

  14. Anatomic Study of Female Sterility of Pinus tabulaeformis Carr.

    Institute of Scientific and Technical Information of China (English)

    Cheng Pengjun; Li Fenglan; Zheng Caixia

    2003-01-01

    The anatomic research on the mutant clone of Pinus tabulaeformis Carr. in the seed orchard in Xingcheng, LiaoningProvince was carried out. The female cone of the mutant clone looked like normal, but its ovules degenerated in the early stage. Thispaper tries to find out the reason and time of ovule abortion. It seems that the ovule abortion is probably caused by female sterilitybecause the microspores of this mutant clone were normal. Through the serial observations on the one-year-old macrosporangiatesand the ovules of two-year-old female cones of mutant and normal clone, it is found that the reason of ovule abortion in mutant cloneis the failure of the mitosis of free nuclei in the female gametophyte, and the time is about in the early April.

  15. Genetic relationships among some Pinus, Picea and Abies species revealed by RAPD markers

    Directory of Open Access Journals (Sweden)

    Kovačević Dragan

    2013-01-01

    Full Text Available Studies were undertaken to identify genetic relationships among ten different species of the family Pinaceae through randomly amplified polymorphic DNA (RAPD markers. Eighteen arbitrary RAPD primers produced 123 fragments of which 107 were polymorphic (87%. The similarity coefficient values varied from 0.34 to 0.67. The highest similarity coefficient was detected between Pinus wallichiana and P. strobus as well as between Picea abies and P. orientalis, and the lowest was detected between threePinus species (P. heldreichii, P. peuce and P. wallichiana and Picea omorika. The analysis of RAPD markers confirmed the genetic relationships among species. GenusPicea is clearly separated from genus Pinus and is closer to genus Abies (A. concolor than to genus Pinus, what confirms up-to-date numerous comparative-morphological, anatomical, chemotaxonomic and molecular results of these closely related genera. Furthermore, on the basis of our results, pine species from different subgenera -Pinus and Strobus are clearly separated. This statement is in agreement with contemporary intrageneric classification of the genus Pinus. [Projekat Ministarstva nauke Republike Srbije, br.173029

  16. A laboratorial study on influence of alkaline and oxidative environment on preservation of Pinus tabulaeformis pollen

    Institute of Scientific and Technical Information of China (English)

    Fang TIAN; Xianyong CAO; Qinghai XU; Yuecong LI

    2009-01-01

    Different sedimentary settings can influence preservation of pollens, which would lead to mis-interpretation of fossil pollen spectrum. This study investigates the influence on the preservation of Pinus tabulaeformis pollen by simulating alkaline and oxidative environment in the laboratory. There was no obvious change in the content ofPinus tabulaeformis pollen while comparing the original with the ones that were immersed with 10% NaOH liquor for ten days, or boiled for five hours, and or boiled with 20%-30% NaOH for one hour,respectively. However, the pollen fossils were obviously corroded and eroded after being boiled with 40% NaOH for one hour and were seriously corroded after five hours. The result indicates that Pinus tabulaeformis pollen is quite durable in alkaline environment and heating condition within a shorter period of time, although alkaline environment has a disadvantage for its preservation. We also tested the influence of oxidation on Pinus tabulae-formis pollen preservation with KMnO4 as oxidant. The result presents that the number of remaining Pinus tabulaeformis pollen grains decreased quickly after being dipped in KMnO4 along with extending the reaction time and reinforcing oxidant. The rate of remnant pollen grains was less than 1% after being dipped with 2% KMnO4 for one hour. It is suggested that oxidative environment has stronger influence on Pinus tabulaeformis pollen preserva tion than alkaline environment.

  17. Crescimento e bromatologia do feijão-bravo (Capparis flexuosa L. em área de Caatinga no Curimataú paraibano, Brasil Growth and bromatology of Capparis flexuosa at Curimatau region, Paraiba state, Brazil

    Directory of Open Access Journals (Sweden)

    Jorge Xavier de Almeida Neto

    2011-06-01

    Full Text Available Este trabalho teve como objetivo caracterizar aspectos relacionados com o crescimento e a bromatologia do feijão-bravo (Capparis flexuosa L. em uma área no Curimataú, Semiárido Paraibano. Para a análise da evolução do crescimento desta espécie formou-se quatro grupos de plantas segundo a classe de altura: GI até 0,50 m; GII de 0,51 a 1 m; GIII de 1,01 a 1,50 m e GIV a >1,51 m. No período de março a dezembro de 2007 determinou-se a altura e o número de ramos dos indivíduos, sendo o diâmetro do caule registrado de março a setembro. Avaliou-se também, a composição bromatológica das folhas e talos do feijão-bravo. Em todos os grupos, o feijão-bravo apresentou crescimento lento no período chuvoso. Entretanto, até o mês de agosto, observou-se o aumento no número de ramos das plantas, independente do grupo estabelecido. No Período amostrado, não se constatou diferença (p > 0,05 entre plantas em termos de diâmetro, em todos os grupos amostrados. Os dados bromatológicos registrados foram os seguintes: matéria orgânica (91,95%; cinzas (8,05%; matéria seca (47,1% e umidade (52,9%. Os teores de proteína bruta (8,13%; fibra bruta (32,32%; extrato etéreo (5,37%; e energia bruta (5.015 cal g-1 do feijão-bravo, dado a estas características, sugerem que esta espécie apresenta potencial para ser utilizada como forrageira.The general aim of this study was to describe the growth and bromatology of Capparis flexuosa L. in an area of Caatinga, Paraiba State, Brazil. To analyze the growth evolution, the plants were divided in four groups according to the height classes: GI until 0.50 m; GII from 0.51 to 1 m; GIII from 1.01 to 1.50 m and GIV above >1.51 m; the plants height, the number of branches and the basal diameter were determined, in the period between March and December, 2007. The bromatological composition of leaves and branches of Capparis flexuosa was evaluated. It was showed, in all groups, that Capparis flexuosa

  18. Quality of water and sediment in streams affected by historical mining, and quality of Mine Tailings, in the Rio Grande/Rio Bravo Basin, Big Bend Area of the United States and Mexico, August 2002

    Science.gov (United States)

    Lambert, Rebecca B.; Kolbe, Christine M.; Belzer, Wayne

    2008-01-01

    The U.S. Geological Survey, in cooperation with the International Boundary and Water Commission - U.S. and Mexican Sections, the National Park Service, the Texas Commission on Environmental Quality, the Secretaria de Medio Ambiente y Recursos Naturales in Mexico, the Area de Proteccion de Flora y Fauna Canon de Santa Elena in Mexico, and the Area de Proteccion de Flora y Fauna Maderas del Carmen in Mexico, collected samples of stream water, streambed sediment, and mine tailings during August 2002 for a study to determine whether trace elements from abandoned mines in the area in and around Big Bend National Park have affected the water and sediment quality in the Rio Grande/Rio Bravo Basin of the United States and Mexico. Samples were collected from eight sites on the main stem of the Rio Grande/Rio Bravo, four Rio Grande/Rio Bravo tributary sites downstream from abandoned mines or mine-tailing sites, and 11 mine-tailing sites. Mines in the area were operated to produce fluorite, germanium, iron, lead, mercury, silver, and zinc during the late 1800s through at least the late 1970s. Moderate (relatively neutral) pHs in stream-water samples collected at the 12 Rio Grande/Rio Bravo main-stem and tributary sites indicate that water is well mixed, diluted, and buffered with respect to the solubility of trace elements. The highest sulfate concentrations were in water samples from tributaries draining the Terlingua mining district. Only the sample from the Rough Run Draw site exceeded the Texas Surface Water Quality Standards general-use protection criterion for sulfate. All chloride and dissolved solids concentrations in water samples were less than the general-use protection criteria. Aluminum, copper, mercury, nickel, selenium, and zinc were detected in all water samples for which each element was analyzed. Cadmium, chromium, and lead were detected in samples less frequently, and silver was not detected in any of the samples. None of the sample concentrations of

  19. Studies on phenolic acids variation in Central European Pinus species 1. Five Polish populations of Pinus mugo Turra and some related forms

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    Tadeusz Krzaczek

    2014-01-01

    Full Text Available Phenolic acids were investigated in the needles of eight different pine populations, five of which were Pinus mugo, two -- P. silvestris and one a critical form described by Neumann as P. uliginosa. Twenty different phenolic acids were detected. Six were discovered in pine needles for the first time (salicylic acid, gentisic acid, γ-resorcylic acid, o-hydroxyphenylacetic acid, β-resorcylic acid, p-hydroxyphenylacetic acid. Each of the investigated taxons was shown to exhibit a characteristic spectrum of phenolic acids. The critical form from the locus classicus of Pinus uliginosa is of intermediate character.

  20. Isoenzymatic variability in progeny of Pinus mugo Turra x Pinus sylvestris L. hybrids from Bór na Czerwonem, in experimental culture

    OpenAIRE

    Maria A. Bobowicz; Władysław Danielewicz

    2014-01-01

    The article describes the genetic structure of 12-year old seedlings. They were grown in an experimental culture representing the progeny of natural hybrids of Pinus mugo Turra and Pinus sylvestris L. from the peatbog Bór na Czerwonem. The studies were carried out on seedlings, employing electrophoresis of enzymatic proteins from winter buds. Parallel isoenzymatic analyses were also conducted for trees of P. mugo Turra from Tatra Mountains and for P. sylvestris L. trees from Białowieża in ord...

  1. Resistência de amendoim-bravo aos herbicidas inibidores da enzima acetolactato sintase Wild poinsettia resistance to acetolactate synthase inhibitor herbicides

    Directory of Open Access Journals (Sweden)

    Ribas A. Vidal

    1999-12-01

    Full Text Available O controle contínuo de plantas daninhas através da aplicação de herbicidas que apresentam atividade em um único local de ação nas plantas favorece a seleção de biótipos resistentes a estes herbicidas, em certas espécies vegetais. Quatro experimentos foram conduzidos em condições casa-de-vegetação, na Faculdade de Agronomia da Universidade Federal do Rio Grande do Sul, com os objetivos de avaliar a ocorrência de resistência aos herbicidas inibidores da enzima acetolactato sintase (ALS em vários biótipos de leiteiro ou amendoim-bravo (Euphorbia heterophylla EPHHL e avaliar a ocorrência de resistência múltipla a herbicidas com atividade em outros locais de ação. Biótipo oriundo de Passo Fundo foi resistente ao imazethapyr, enquanto biótipo oriundo de Porto Alegre foi suscetível. O biótipo de Passo Fundo apresentou resistência cruzada aos herbicidas imidazolinonas: imazapyr, imazaquin e imazethapyr; sulfoniluréias: chlorimuron, nicosulfuron e metsulfuron; e sulfonanilida: flumetsulan. Este biótipo não foi resistente aos herbicidas com os seguintes mecanismos de ação: inibidores de EPSPs, mimetizadores de auxina, inibidores dos fotossistemas I e II e inibidores de PROTOX. A confirmação de resistência aos inibidores de ALS em biótipos oriundos de Nãome-Toque, Passo Fundo e Rio Pardo sugere ampla dispersão no Rio Grande do Sul de resistência de E. heterophylla aos herbicidas deste mecanismo de ação.The continuous weed control with herbicides of only one site of action selects biotypes resistant to these herbicides. Four experiments were conducted in greenhouse of UFRGS, Brazil, to confirm the occurence of wild poinsettia (Euphorbia heterophylla biotypes resistance to herbicides inhibitors of acetholactate synthase (ALS, and to determine whether there was cross resistance to herbicides with other site of action. A biotype from Passo Fundo -RS was resistant to imazethapyr, whereas a biotype from Porto Alegre -RS

  2. Design and methods for the Better Resiliency Among Veterans and non-Veterans with Omega-3's (BRAVO) study: A double blind, placebo-controlled trial of omega-3 fatty acid supplementation among adult individuals at risk of suicide.

    Science.gov (United States)

    Marriott, Bernadette P; Hibbeln, Joseph R; Killeen, Therese K; Magruder, Kathryn M; Holes-Lewis, Kelly; Tolliver, Bryan K; Turner, Travis H

    2016-03-01

    Suicide remains the 10th leading cause of death among adults in the United States (U.S.). Annually, approximately 30 per 100,000 U.S. military Veterans commit suicide, compared to 14 per 100,000 U.S. civilians. Symptoms associated with suicidality can be treatment resistant and proven-effective pharmaceuticals may have adverse side-effects. Thus, a critical need remains to identify effective approaches for building psychological resiliency in at-risk individuals. Omega-3 highly unsaturated fatty acids (n-3 HUFAs) are essential nutrients, which must be consumed in the diet. N-3 HUFAs have been demonstrated to reduce symptoms of depression, anxiety, and impulsivity - which are associated with suicide risk. Here we present the design and methods for the Better Resiliency Among Veterans and non-Veterans with Omega-3's (BRAVO) study, which is a double blind, randomized, controlled trial among individuals at risk of suicide of an n-3 HUFA versus placebo supplementation in the form of all natural fruit juice beverages. The BRAVO study seeks to determine if dietary supplementation with n-3 HUFAs reduces the risk for serious suicidal behaviors, suicidal thinking, negative emotions, and symptoms associated with suicide risk. Sub-analyses will evaluate efficacy in reducing depressive symptoms, alcohol, and nicotine use. A sub-study utilizes functional magnetic resonance imaging (fMRI) to evaluate the neuropsychological and neurophysiological effects of n-3 HUFAs. We also outline selection of appropriate proxy outcome measures for detecting response to treatment and collection of ancillary data, such as diet and substance use, that are critical for interpretation of results.

  3. Evidence of recent deep magmatic activity at Cerro Bravo-Cerro Machín volcanic complex, central Colombia. Implications for future volcanic activity at Nevado del Ruiz, Cerro Machín and other volcanoes

    Science.gov (United States)

    Londono, John Makario

    2016-09-01

    In the last nine years (2007-2015), the Cerro Bravo-Cerro Machín volcanic complex (CBCMVC), located in central Colombia, has experienced many changes in volcanic activity. In particular at Nevado del Ruiz volcano (NRV), Cerro Machin volcano (CMV) and Cerro Bravo (CBV) volcano. The recent activity of NRV, as well as increasing seismic activity at other volcanic centers of the CBCMVC, were preceded by notable changes in various geophysical and geochemical parameters, that suggests renewed magmatic activity is occurring at the volcanic complex. The onset of this activity started with seismicity located west of the volcanic complex, followed by seismicity at CBV and CMV. Later in 2010, strong seismicity was observed at NRV, with two small eruptions in 2012. After that, seismicity has been observed intermittently at other volcanic centers such as Santa Isabel, Cerro España, Paramillo de Santa Rosa, Quindío and Tolima volcanoes, which persists until today. Local deformation was observed from 2007 at NRV, followed by possible regional deformation at various volcanic centers between 2011 and 2013. In 2008, an increase in CO2 and Radon in soil was observed at CBV, followed by a change in helium isotopes at CMV between 2009 and 2011. Moreover, SO2 showed an increase from 2010 at NRV, with values remaining high until the present. These observations suggest that renewed magmatic activity is currently occurring at CBCMVC. NRV shows changes in its activity that may be related to this new magmatic activity. NRV is currently exhibiting the most activity of any volcano in the CBCMVC, which may be due to it being the only open volcanic system at this time. This suggests that over the coming years, there is a high probability of new unrest or an increase in volcanic activity of other volcanoes of the CBCMVC.

  4. Bravo, aktrissa! / interv. Galina Volkova

    Index Scriptorium Estoniae

    2004-01-01

    Vene Draamateatris käivad vene näitekirjaniku N. Ptushkina komöödia "Braavo, Laurensia" proovid, millega tähistatakse näitlejanna L. Golovataja juubelit. Küsimustele vastavad näidendi lavastaja E. Toman, näitlejad A. Garejev, J. Tarassenko, L. Shinkarjova ja lavastaja abi S. Krassman

  5. Bravo! Neurology at the opera.

    Science.gov (United States)

    Matthews, Brandy R

    2010-01-01

    Opera is a complex musical form that reflects the complexity of the human condition and the human brain. This article presents an introduction to the portrayal of medical professionals in opera, including one neurologist, as well as two characters in whom neurological disease contributes to the action of the musical drama. Consideration is also given to the neuroanatomy and neuropathology of opera singers with further speculation regarding the neural underpinnings of the passion of opera's audience.

  6. Caracterização dos taninos condensados das espécies maniçoba (Manihot pseudoglazovii), flor-de-seda (Calotropis procera), feijão-bravo (Capparis flexuosa, L) e jureminha (Desmanthus virgatus) Characterization of condensed tannin of the species maniçoba (Manihot pseudoglaziovii), flor-de-seda (Calotropis procera), feijão-bravo (Capparis flexuosa) and jureminha (Desmanthus virgatus)

    OpenAIRE

    S.E.S.B.S. Cruz; BEELEN, P. M. G.; Silva,D.S.; PEREIRA, W. E.; Beelen, R; Beltrão,F.S.

    2007-01-01

    Caracterizaram-se os taninos condensados (TC) e determinaram-se a composição bromatológica e a digestibilidade in vitro da matéria seca (DIVMS) das espécies maniçoba (Manihot pseudoglazovii Pax & Hoffman), flor-de-seda (Calotropis procera), feijão-bravo (Capparis flexuosa, L) e jureminha (Desmanthus virgatus (L.) Willd). As concentrações em tanino solúvel, tanino ligado ao resíduo e tanino total (TT) das espécies foram determinadas pelo método butanol-HCL e a adstringência pelo método de difu...

  7. Caracterização dos taninos condensados das espécies maniçoba (Manihot pseudoglazovii, flor-de-seda (Calotropis procera, feijão-bravo (Capparis flexuosa, L e jureminha (Desmanthus virgatus Characterization of condensed tannin of the species maniçoba (Manihot pseudoglaziovii, flor-de-seda (Calotropis procera, feijão-bravo (Capparis flexuosa and jureminha (Desmanthus virgatus

    Directory of Open Access Journals (Sweden)

    S.E.S.B.S. Cruz

    2007-08-01

    Full Text Available Caracterizaram-se os taninos condensados (TC e determinaram-se a composição bromatológica e a digestibilidade in vitro da matéria seca (DIVMS das espécies maniçoba (Manihot pseudoglazovii Pax & Hoffman, flor-de-seda (Calotropis procera, feijão-bravo (Capparis flexuosa, L e jureminha (Desmanthus virgatus (L. Willd. As concentrações em tanino solúvel, tanino ligado ao resíduo e tanino total (TT das espécies foram determinadas pelo método butanol-HCL e a adstringência pelo método de difusão radial. Para a DIVMS, foi utilizado método de dois estágios. Foram observadas diferenças entre as espécies (PCondensed tannins (CT of the species maniçoba (Manihot pseudoglaziovii, flor-de-seda (Calotropis procera, feijão-bravo (Capparis flexuosa and jureminha (Desmanthus virgatus were characterized and the chemical composition and in vitro dry matter digestibility (IVDMD of those species were determined. Concentration of soluble tannin (ST, bound tannin (BT and total tannin (TT of the plants was determined using butanol-HCL method; astringency was determined, using radial diffusion method, and the IVDMD was determined using two-stages method. Concentration and astringency of condensed tannins varied between species (P<0.01. Jureminha was the species that presented the highest value (2.4% TT and 13.7-3 astringency. No tannins were detected in flor-de-seda. Crude protein was higher than 16% in all studied species, and the ADF and lignin values were lower than 39 and 15%, respectively. The IVDMD was low in jureminha (43% and high in flor-de-seda (80%. The correlation between IVDMD and TT was low (r²=0.097.

  8. Investigation on Effect of Forestation by Mixing Pinus massoniana with Schima superba

    Institute of Scientific and Technical Information of China (English)

    HUANGWenchao; HUANGLili

    2004-01-01

    Since forestation of pure forest of Pinus massoniana is liable to suffer from pest calamity, soil erosion, decrease of soil fertility, and difficulty in wood production in the hilly areas of southern China, we conducted an investigation on the three types of forests in Gao'an County of Jiangxi Province, namely, the pure forest of Pinus massoniana, the pure forest of Schima superba, and the mixed forest consisting of the two species, setting up standard stand, measuring and studying the growing stock, biomass, leaf area, roots,soil, vegetation, pests, litters, soil erosion, microclimate, etc., with the following results. ① The average height, diameter at chest height, and volume of the mixed forest are higher than those of the pure forest of Pinus massoniana by 30.9%, 31.7% and 10.6%, respectively. ② The biomass of the mixed forest is 2.24 times as much as that of the pure forest of Pinus massoniana. ③ The litters of the mixed forest is 3.37 times and 1.96 times as much as the litters of the pure forest of Pinus massoniana and the pure forest of Schima superba respectively. ③ The occurrence of pest calamity per tree is lower and the conditions of soil erosion and microclimate are better than those of the pure forest ofPinus massoniana. ④ The mixed forest of the two species is an excellent mixed type of needle-leaf and deciduous forest worth being popularized in China's subtropical areas, especially in the low hilly areas. It is recommended in plantation that the mixed patterns be between trees, lines, small blocks, or scattered-dots, the ratio of mixture of Pinus massoniana and Schima superba be 5 to 1, or 3 to 1, and the initial space between trees be 1.2, 1.5, or 2.0 m while the initial density be 6 944 to 4 440 trees per square hectare.

  9. Molecular Characterization of a Dehydroascorbate Reductase from Pinus bungeana

    Institute of Scientific and Technical Information of China (English)

    Hai-Ling Yang; Ying-Ru Zhao; Cai-Ling Wang; Zhi-Ling Yang; Qing-Yin Zeng; Hai Lu

    2009-01-01

    Dehydroascorbate reductase (DHAR) plays a critical role in the ascorbate-glutathione recycling reaction for most higher plants. To date, studies on DHAR in higher plants have focused largely on Arabidopsis and agricultural plants, and there is virtually no information on the molecular characteristics of DHAR in gymnosperms. The present study reports the cloning and characteristics of a DHAR (PbDHAR) from a pine, Pinus bungeana Zucc. ex Endl. The PbDHAR gene encodes a protein of 215 amino acid residues with a calculated molecular mass of 24.26 kDa. The predicted 3-D structure of PbDHAR showed a typical glutathione S-transferase fold. Reverse transcription-polymerase chain reaction revealed that the PbDHAR was a constitutive expression gene in P. bungeana. The expression level of PbDHAR mRNA in P. bungeana seedlings did not show significant change under high temperature stress. The recombinant PbDHAR was overexpressed in Escherichia coll following purification with affinity chromatography. The recombinant PbDHAR exhibited enzymatic activity (19.84μmol/min per mg) and high affinity (a K_m of 0.08 mM) towards the substrates dehydroascorbate (DHA). Moreover, the recombinant PbDHAR was a thermostable enzyme, and retained 77% of its initial activity at 55 ℃. The present study is the first to provide a detailed molecular characterization of the DHAR in P. bungeana.

  10. Urbanization in China drives soil acidification of Pinus massoniana forests.

    Science.gov (United States)

    Huang, Juan; Zhang, Wei; Mo, Jiangming; Wang, Shizhong; Liu, Juxiu; Chen, Hao

    2015-01-01

    Soil acidification instead of alkalization has become a new environmental issue caused by urbanization. However, it remains unclear the characters and main contributors of this acidification. We investigated the effects of an urbanization gradient on soil acidity of Pinus massoniana forests in Pearl River Delta, South China. The soil pH of pine forests at 20-cm depth had significantly positive linear correlations with the distance from the urban core of Guangzhou. Soil pH reduced by 0.44 unit at the 0-10 cm layer in urbanized areas compared to that in non-urbanized areas. Nitrogen deposition, mean annual temperature and mean annual precipitation were key factors influencing soil acidification based on a principal component analysis. Nitrogen deposition showed significant linear relationships with soil pH at the 0-10 cm (for ammonium N(NH4+(-N)), P urbanization gradient, instead their levels were higher in urban than in urban/suburban area at the 0-10 cm layer. Our results suggested N deposition particularly under the climate of high temperature and rainfall, greatly contributed to a significant soil acidification occurred in the urbanized environment.

  11. Effect of trace elements on growth of Pinus tabulaeformis seedling

    Institute of Scientific and Technical Information of China (English)

    2002-01-01

    The stimulative effect of trace elements on seed germination and seedling growth of Pinus tabulaeformis was tested. The experiments were carried out on seed soak and topdressing with different trace elements and varied concentrat ions at the nursery of Gardens Research Institute, Harbin, in 2000-2001. The experimental results showed that soaking seed with 1% and 0.2% concentrations of Mn element produced best result for seed germination, and the germination rate was increased by 9%~19% for the seeds treated with 1% concentration and 12%~14% for the seeds treated with 0.2% concentration compared with the control group. The seeds treated with boron element had lowest germination rate. For trace element topdressing, Mn and Mo elements presented good result for seedling growth and th e treatment with low concentration was even better. The height or chlorophyll co ntent of the seedlings with spray of low-concentration Mn and Mo element was muc h higher than that of untreated ones. In the contrast to the treating method of seed soak, topdressing (application of spraying on foliage) had evident effect o n seedling growth.

  12. Bending of Pinus jeffreyi in response to wind

    Directory of Open Access Journals (Sweden)

    Stephen H. Bullock

    2015-12-01

    Full Text Available Aim of study: To evaluate the degree of trunk sway in relation to wind velocity, with varying temporal integration and to compare this relation among seasons.Area of study: Sierra de Juárez, Baja California, MéxicoMaterials and Methods: Displacements of a 19 m tall Jeffrey pine tree were recorded at 6 m from a three dimensional digital compass during one year, at c. 4 Hz. Adjacent wind speed at 6 m was recorded at 1 Hz.Main results: Sway was essentially unaffected by wind in the same second  but increasing dependence of cumulative displacement on average sustained wind speed was found for intervals of 1 to 60 minutes (r2 up to 0.89.  The relation is generally log-linear but apparently differs in parameters between seasons.Research highlights: Wind-sway relations are clear from integration of several-to-many minutes. However, to estimate cumulative stress, sub-second data on sway are essential.  Sub-second, precision measurements of sway can be registered from small, inexpensive sensors.Keywords: biomechanics; Pinus jeffreyi; seasonality; stress accumulation; time series; tree bending.

  13. Genetic transformation of Pinus taeda by particle bombardment

    Institute of Scientific and Technical Information of China (English)

    2002-01-01

    A protocol is presented for genetically engineering loblolly pine (Pinus taeda L.) using particle bombardment. This protocol enabled the routine transformation of loblolly pine plants that were previously difficult to transform. Mature zygotic embryos were used to be bombarded and to generate organogenic callus and transgenic regenerated plants. Plasmid pB48.215 DNA contained a synthetic Bacillus thuringiensis (B.t.) cryIAc coding sequence flanked by the double cauliflower mosaic virus (CaMV) 35S promoter and nopaline synthase (Nos) terminator sequences, and the selectable marker gene, neomycin phosphotransferase II (nptII) controlled by the promoter of the nopaline synthase gene was introduced into loblolly pine tissues by particle bombardment. The transformed tissues were proliferated and selected by kanamycin resistance conferred by the introduced NPTII gene. Shoot regeneration was induced from the kanamycin-resistant callus, and transgenic plantlets were then produced. The presence of the introduced genes in the transgenic loblolly pine plants was confirmed by polymerase chain reactions (PCR) analysis, by Southern blot analysis, and insect feeding assays. The recovered transgenic plants were acclimatized and then established in soil.

  14. Dynamics of monoterpene emissions in Pinus sylvestris during early spring

    Energy Technology Data Exchange (ETDEWEB)

    Baeck, J.; Hari, P.; Juurola, E. [Department of Forest Ecology, University of Helsinki (Finland); Hakola, H. [Finnish Meteorological Institute, Research and Development, Helsinki (Finland); Kulmala, M. [Department of Physical Sciences, University of Helsinki (Finland)

    2005-07-01

    The seasonal dynamics of biogenic volatile organic compound (BVOC) emissions, which can be related to the formation and growth of secondary organic aerosols, represent an important but at the present poorly understood linkage between vegetation activity and climate. Although a close relationship between photosynthesis and terpenoid emissions has been proposed, high monoterpene emission rates for Scots pine shoots (Pinus sylvestris) are frequently recorded during spring, in times when photosynthetic activity is strongly inhibited due to inherent seasonal restrictions. We suggest that terpenoid emissions are related to either photosynthesis or photorespiration for precursors for terpenoid biosynthesis. We developed two dynamic models describing temporal fluctuations in Scots pine monoterpene emissions, calculating the emissions by using CO{sub 2} exchange and ambient climate data. The models accurately predicted the measured monoterpene flux, and especially in March-April, during the time when photosynthesis was negligible and ambient temperatures were between -5 and +15 C, a good agreement was found with measured emissions and the model involving photorespiration. (orig.)

  15. Antioxidative properties of the essential oil from Pinus mugo.

    Science.gov (United States)

    Grassmann, Johanna; Hippeli, Susanne; Vollmann, Renate; Elstner, Erich F

    2003-12-17

    The essential oil from Pinus mugo (PMEO) was tested on its antioxidative capacity. For this purpose, several biochemical test systems were chosen (e.g., the Fenton System, the xanthine oxidase assay, or the copper-induced oxidation of low-density lipoprotein (LDL)). The results show that there is moderate or weak antioxidative activity when tested in aqueous environments, like in the Fenton system, xanthine oxidase induced superoxide radical formation, or in the HOCl driven fragmentation of 1-aminocyclopropane-1-carboxylic acid (ACC). In contrast, when tested in more lipophilic environments (e.g., the ACC-cleavage by activated neutrophils in whole blood) the PMEO exhibits good antioxidative activity. PMEO does also show good antioxidative capacity in another lipophilic test system (i.e., the copper induced oxidation of LDL). Some components of PMEO (i.e., Delta(3)-carene, camphene, alpha-pinene, (+)-limonene and terpinolene) were also tested. As the PMEO, they showed weak or no antioxidant activity in aqueous environments, but some of them were effective antioxidants regarding ACC-cleavage by activated neutrophils in whole blood or copper-induced LDL-oxidation. Terpinolene, a minor component of PMEO, exhibited remarkable protection against LDL-oxidation.

  16. MICROBIOTA OF PINUS POLLEN AS ADJUVANT FACTOR OF ALLERGY

    Directory of Open Access Journals (Sweden)

    Tetiana Shevtsova

    2016-06-01

    Full Text Available Bacteria, their endotoxin and mold found on pollen can be a reason of respiratory symptoms in sensitized individuals. This question concerns an anemophilous pollen more acute. In this work quantitative by dilution plating method and qualitative microbial analysis by MALDI-TOF MS Biotyper of pollen and other plants organs of Pinus sylvestris L., P. nigra Arnold, P. mugo Turra, P. armandii Franch., P. wallichiana A.B. Jacks from Nitra, Slovakia are performed which shows quantitative and species differences in mesophilic aerobic (0.00-6.27 log cfu/g and anaerobic bacteria (0.00-3.70 log cfu/g, enterococci (0.00 log cfu/g, coliform bacteria (0.00-5.29 log cfu/g, lactobacilli (0.00-4.20 log cfu/g, microscopic fungi and yeasts (2.60-5.29 log cfu/g content. Representatives of Pseudomonas (14, Bacillus (2, Acinetobacter (1, Arthrobacter (1, Pantoea (1, Klebsiella (1, Penicillium (6, Aspergillus (4, Cladosporium (1, Debaryomyces (1 genera were revealed on pine trees. The allergenic potential of the identified association of microorganisms on pollen has been evaluated based on published data. The results may be useful for aerobiologists, allergists and microbiologists, at least at the local level.

  17. [Soil microbial functional diversity of different altitude Pinus koraiensis forests].

    Science.gov (United States)

    Han, Dong-xue; Wang, Ning; Wang, Nan-nan; Sun, Xue; Feng, Fu-juan

    2015-12-01

    In order to comprehensively understand the soil microbial carbon utilization characteristics of Pinus koraiensis forests, we took the topsoil (0-5 cm and 5-10 cm) along the 700-1100 m altitude in Changbai Mountains and analyzed the vertical distributed characteristics and variation of microbial functional diversity along the elevation gradient by Biolog microplate method. The results showed that there were significant differences in functional diversity of microbial communities at different elevations. AWCD increased with the extension of incubation time and AWCD at the same soil depth gradually decreased along with increasing altitude; Shannon, Simpson and McIntosh diversity index also showed the same trend with AWCD and three different diversity indices were significantly different along the elevation gradient; Species diversity and functional diversity showed the same variation. The utilization intensities of six categories carbon sources had differences while amino acids were constantly the most dominant carbon source. Principal component analysis (PCA) identified that soil microbial carbon utilization at different altitudes had obvious spatial differentiation, as reflected in the use of carbohydrates, amino acids and carboxylic acids. In addition, the cluster of the microbial diversity indexes and AWCD values of different altitudes showed that the composition of vegetation had a significant impact on soil microbial composition and functional activity.

  18. Biocontrol of Fusarium circinatum Infection of Young Pinus radiata Trees

    Directory of Open Access Journals (Sweden)

    Eugenia Iturritxa

    2017-01-01

    Full Text Available Pitch canker, caused by the fungus Fusarium circinatum, is a major disease of Pinus radiata currently controlled to some extent in nurseries by good hygiene and application of synthetic fungicides. The aim of this study was to evaluate alternative strategies to control fungal infections in nurseries and young pine plantations. The antagonistic effects of biocontrol bacteria and essential oils against F. circinatum in vitro and in young P. radiata trees were assessed. Pseudomonas fluorescens, Erwinia billingiae, and Bacillus simplex reduced the growth of the fungus in vitro by 17%–29%, and decreased the density of the mycelial mat. In young P. radiata trees, the length of F. circinatum lesions was reduced by 22%–25% by the same bacterial strains. Direct application of cinnamon and/or clove essential oils to wounds in stems of two-year-old P. radiata trees also limited the damage caused by F. circinatum. Lesion length was reduced by 51% following treatment with cinnamon oil (10% v/v, and by 45% following treatment with clove oil (15% v/v or a combination of both oils. However, the oils were toxic to younger trees. The biocontrol bacteria and essential oils show promise as prophylactic treatments to reduce the devastating effects of F. circinatum on P. radiata.

  19. Urbanization in China drives soil acidification of Pinus massoniana forests

    Science.gov (United States)

    Huang, Juan; Zhang, Wei; Mo, Jiangming; Wang, Shizhong; Liu, Juxiu; Chen, Hao

    2015-09-01

    Soil acidification instead of alkalization has become a new environmental issue caused by urbanization. However, it remains unclear the characters and main contributors of this acidification. We investigated the effects of an urbanization gradient on soil acidity of Pinus massoniana forests in Pearl River Delta, South China. The soil pH of pine forests at 20-cm depth had significantly positive linear correlations with the distance from the urban core of Guangzhou. Soil pH reduced by 0.44 unit at the 0-10 cm layer in urbanized areas compared to that in non-urbanized areas. Nitrogen deposition, mean annual temperature and mean annual precipitation were key factors influencing soil acidification based on a principal component analysis. Nitrogen deposition showed significant linear relationships with soil pH at the 0-10 cm (for ammonium N (-N), P deposition particularly under the climate of high temperature and rainfall, greatly contributed to a significant soil acidification occurred in the urbanized environment.

  20. Effects of "short" photoperiods on seedling growth of Pinus brutia.

    Science.gov (United States)

    Iakovoglou, V; Radoglou, K; Kostopoulou, P; Dini-Papanastasi, O

    2012-03-01

    This study investigated how nurseries could benefit by inducing "short" photoperiods as low as 4 hr to produce "better" seedlings characterized by more vigorous roots; a substantial feature to overcome transplanting stress. The carryover effect of the photoperiod was also investigated on seedlings that grew for 30 days more underthe consistent 14 hr photoperiod. Seedlings of Pinus brutia were subjected to 4, 6, 8 and 14 hr photoperiod for 3 week. Fifteen seedlings were used to evaluate the leaf area, the root and shoot dry weight and their ratio. Six and sixteen seedlings were used to evaluate the shoot electrolyte leakage and the root growth potential, respectively. Based on the results, the 6 and 8 hr photoperiod indicated greater root allocation (4.8 and 4.9 mg, respectively) and chlorophyll content (3.7 and 4.4, respectively). They also indicated greater leaf area values (3.3 and 3.5 cm2, respectively) along with the 14 hr (3.4 cm2). The photoperiod effect continued even after seedlings were subjected at consistent photoperiod. Overall, "short" photoperiods could provide "better" P. brutia seedlings to accommodate immediate massive reforestation and afforestation needs.

  1. Aboveground Tree Biomass for Pinus ponderosa in Northeastern California

    Directory of Open Access Journals (Sweden)

    Todd A. Hamilton

    2013-03-01

    Full Text Available Forest managers need accurate biomass equations to plan thinning for fuel reduction or energy production. Estimates of carbon sequestration also rely upon such equations. The current allometric equations for ponderosa pine (Pinus ponderosa commonly employed for California forests were developed elsewhere, and are often applied without consideration potential for spatial or temporal variability. Individual-tree aboveground biomass allometric equations are presented from an analysis of 79 felled trees from four separate management units at Blacks Mountain Experimental Forest: one unthinned and three separate thinned units. A simultaneous set of allometric equations for foliage, branch and bole biomass were developed as well as branch-level equations for wood and foliage. Foliage biomass relationships varied substantially between units while branch and bole biomass estimates were more stable across a range of stand conditions. Trees of a given breast height diameter and crown ratio in thinned stands had more foliage biomass, but slightly less branch biomass than those in an unthinned stand. The observed variability in biomass relationships within Blacks Mountain Experimental Forest suggests that users should consider how well the data used to develop a selected model relate to the conditions in any given application.

  2. Antioxidant, antimutagenic, and antitumor effects of pine needles (Pinus densiflora).

    Science.gov (United States)

    Kwak, Chung Shil; Moon, Sung Chae; Lee, Mee Sook

    2006-01-01

    Pine needles (Pinus densiflora Siebold et Zuccarini) have long been used as a traditional health-promoting medicinal food in Korea. To investigate their potential anticancer effects, antioxidant, antimutagenic, and antitumor activities were assessed in vitro and/or in vivo. Pine needle ethanol extract (PNE) significantly inhibited Fe(2+)-induced lipid peroxidation and scavenged 1,1-diphenyl- 2-picrylhydrazyl radical in vitro. PNE markedly inhibited mutagenicity of 2-anthramine, 2-nitrofluorene, or sodium azide in Salmonella typhimurium TA98 or TA100 in Ames tests. PNE exposure effectively inhibited the growth of cancer cells (MCF-7, SNU-638, and HL-60) compared with normal cell (HDF) in 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. In in vivo antitumor studies, freeze-dried pine needle powder supplemented (5%, wt/wt) diet was fed to mice inoculated with Sarcoma-180 cells or rats treated with mammary carcinogen, 7,12-dimethylbenz[a]anthracene (DMBA, 50 mg/kg body weight). Tumorigenesis was suppressed by pine needle supplementation in the two model systems. Moreover, blood urea nitrogen and aspartate aminotransferase levels were significantly lower in pine needle-supplemented rats in the DMBA-induced mammary tumor model. These results demonstrate that pine needles exhibit strong antioxidant, antimutagenic, and antiproliferative effects on cancer cells and also antitumor effects in vivo and point to their potential usefulness in cancer prevention.

  3. Antinociceptive and antiinflammatory activities of pine (Pinus densiflora) pollen extract.

    Science.gov (United States)

    Choi, Eun-Mi

    2007-05-01

    The study aimed to evaluate the antinociceptive and antiinflammatory activity of pine (Pinus densiflora) pollen in mice. The antinociceptive activity was determined using acetic acid-induced abdominal constriction and formalin-induced licking, and the hot plate test. Antiinflammatory effects were evaluated using carrageenan- and formalin-induced paw edema, and arachidonic acid-induced ear edema in mice. The ethanol extract of pine pollen (100 and 200 mg/kg, p.o.) produced a significant inhibition of both phases of the formalin pain test in mice, a reduction in mouse writhing induced by acetic acid and an elevation of the pain threshold in the hot plate test in mice. The pine pollen extract also produced a significant inhibition of carrageenan- and formalin-induced paw edema as well as arachidonic acid-induced ear edema in mice. The inhibitions were similar to those produced by aminopyrine and indomethacin, p.o. The different polyphenols found in pine pollen could account for the antinociceptive and antiinflammatory actions. The results obtained indicate that the extract possesses analgesic and antiinflammatory effects.

  4. An allelopathic substance in red pine needles (Pinus densiflora).

    Science.gov (United States)

    Kato-Noguchi, Hisashi; Fushimi, Yoshiko; Shigemori, Hideyuki

    2009-03-01

    Aqueous methanol extracts of red pine (Pinus densiflora) needles inhibited the growth of roots and shoots of cress (Lepidium sativum), lettuce (Lactuca sativa), alfalfa (Medicago sativa), ryegrass (Lolium multiflorum), timothy (Pheleum pratense), Digitaria sanguinalis and Echinochloa crus-galli. Increasing the extract concentration increased inhibition, suggesting that the pine needles may have growth inhibitory substances and possess allelopathic potential. The aqueous methanol extract of the pine needles was purified, and a main inhibitory substance was isolated and determined by spectral data as 9alpha,13beta-epidioxyabeit-8(14)en-18-oic acid. This substance inhibited root and shoot growth of cress and Echinochloa crus-galli seedlings at concentrations greater than 0.1 mM. The endogenous concentration of the substance was 0.13 mmol/kg pine needle. These results suggest that 9alpha,13beta-epidioxyabeit-8(14)en-18-oic acid may contribute to the growth inhibitory effect of the pine needles and may play an important role in the allelopathy of red pine.

  5. Paleoclimatic implications of glacial and postglacial refugia for Pinus pumila in western Beringia

    Science.gov (United States)

    Anderson, Patricia M.; Lozhkin, Anatoly V.; Solomatkina, Tatiana B.; Brown, Thomas A.

    2010-03-01

    Palynological results from Julietta Lake currently provide the most direct evidence to support the existence of a glacial refugium for Pinus pumila in mountains of southwestern Beringia. Both percentages and accumulation rates indicate the evergreen shrub survived until at least ˜ 19,000 14C yr BP in the Upper Kolyma region. Percentage data suggest numbers dwindled into the late glaciation, whereas pollen accumulation rates point towards a more rapid demise shortly after ˜ 19,000 14C yr BP. Pinus pumila did not re-establish in any great numbers until ˜ 8100 14C yr BP, despite the local presence ˜ 9800 14C yr BP of Larixdahurica, which shares similar summer temperature requirements. The postglacial thermal maximum (in Beringia ˜ 11,000-9000 14C yr BP) provided Pinus pumila shrubs with equally harsh albeit different conditions for survival than those present during the LGM. Regional records indicate that in this time of maximum warmth Pinus pumila likely sheltered in a second, lower-elevation refugium. Paleoclimatic models and modern ecology suggest that shifts in the nature of seasonal transitions and not only seasonal extremes have played important roles in the history of Pinus pumila over the last ˜ 21,000 14C yr BP.

  6. Paleoclimatic implications of glacial and postglacial refugia for Pinus pumila in western Beringia

    Energy Technology Data Exchange (ETDEWEB)

    Anderson, P M; Lozhkin, A V; Solomatkina, T B; Brown, T A

    2010-02-05

    Palynological results from Julietta Lake currently provide the most direct evidence to support the existence of a glacial refugium for Pinus pumila in mountains of southwestern Beringia. Both percentages and accumulation rates indicate the evergreen shrub survived until at least {approx}19,000 14C yr B.P. in the Upper Kolyma region. Percentage data suggest numbers dwindled into the late glaciation, whereas pollen accumulation rates point towards a more rapid demise shortly after {approx}19,000 14C yr B.P. Pinus pumila did not re-establish in any great numbers until {approx}8100 14C yr B.P., despite the local presence {approx}9800 14C yr B.P. of Larix dahurica, which shares similar summer temperature requirements. The postglacial thermal maximum (in Beringia {approx}11,000-9000 14C yr B.P.) provided Pinus pumila shrubs with equally harsh albeit different conditions for survival than those present during the LGM. Regional records indicate that in this time of maximum warmth Pinus pumila likely sheltered in a second, lower-elevation refugium. Paleoclimatic models and modern ecology suggest that shifts in the nature of seasonal transitions and not only seasonal extremes have played important roles in the history of Pinus pumila over the last {approx}21,000 14C yr B.P.

  7. Tree mortality patterns following prescribed fire for Pinus and Abies across the southwestern United States

    Science.gov (United States)

    van Mantgem, Philip J.; Nesmith, Jonathan C. B.; Keifer, MaryBeth; Brooks, Matthew

    2012-01-01

    The reintroduction of fire to historically fire-prone forests has been repeatedly shown to reduce understory fuels and promote resistance to high severity fire. However, there is concern that prescribed fire may also have unintended consequences, such as high rates of mortality for large trees and fire-tolerant Pinus species. To test this possibility we evaluated mortality patterns for two common genera in the western US, Pinus and Abies, using observations from a national-scale prescribed fire effects monitoring program. Our results show that mortality rates of trees >50 DBH were similar for Pinus (4.6% yr-1) and Abies (4.0% yr-1) 5 years following prescribed fires across seven sites in the southwestern US. In contrast, mortality rates of trees >50 cm DBH differed between Pinus (5.7% yr-1) and Abies (9.0% yr-1). Models of post-fire mortality probabilities suggested statistically significant differences between the genera (after including differences in bark thickness), but accounting for these differences resulted in only small improvements in model classification. Our results do not suggest unusually high post-fire mortality for large trees or for Pinus relative to the other common co-occurring genus, Abies, following prescribed fire in the southwestern US.

  8. Atividade da acetolactato sintase de plantas de milho e de amendoim-bravo (Euphorbia heterophylla resistentes e suscetíveis ao imazaquin ALS activity of wild poinsettia (Euphorbia heterophylla and corn (Zea mays resistant and susceptible to imazaquin

    Directory of Open Access Journals (Sweden)

    M.F. Oliveira

    2002-04-01

    Full Text Available O amendoim-bravo, (Euphorbia heterophylla é uma importante planta daninha em mais de 56 países, inclusive no Brasil, onde tem sido relatado o aparecimento de populações resistentes aos herbicidas inibidores da acetolactato sintase (ALS. O objetivo do trabalho foi avaliar o efeito do herbicida imazaquin na atividade da ALS extraída das plantas de milho e amendoim-bravo, resistentes e suscetíveis ao produto. Sementes de dois genótipos de milho e vários biótipos de amendoim-bravo provenientes de diferentes regiões agrícolas brasileiras foram cultivadas em casa de vegetação por 21 dias. A atividade da ALS extraída das folhas das plantas foi determinada na presença de doses de imazaquin. A etapa de purificação da enzima foi substituída por uma centrifugação de 2.800 rpm por dois minutos. Equações de regressão linear para absorbância em função do log da concentração de imazaquin foram ajustadas para cada população, visando obtenção do I50. A dose de imazaquin necessária para inibir 50% da atividade da ALS (I50 na variedade de milho Pioneer 3162 IR (I50 260 µM foi 4.333 vezes maior que a dose requerida pela BRS 473 (0,06 µM, a qual é suscetível ao imazaquin. Os biótipos de amendoim-bravo provenientes do Rio Grande do Sul apresentaram valores de I50 de 1.961,3 e 13,8 mM para os biótipos resistentes e suscetíveis, respectivamente. Os biótipos provenientes de Cafelândia e Maringá (PR e Viçosa (MG apresentaram valores de I50 maiores que 5.000 mM para os biótipos resistentes e maiores que 1.000 mM para os suscetíveis. O amendoim-bravo coletado na Embrapa Milho e Sorgo, em área que nunca foi tratada com herbicidas inibidores da ALS, apresentou I50 de 12,2 mM. Conclui-se que a medida in vitro da atividade da ALS é um método sensível para determinação da presença de biótipos resistentes à ação do herbicida imazaquin. A etapa de purificação da ALS pode ser substituída por um método que envolve uma r

  9. Microsporogenesis and meiotic behavior in nine species of the genus Pinus

    Institute of Scientific and Technical Information of China (English)

    Hui-Sheng DENG; Da-Ming ZHANG; De-Yuan HONG; Cheng-Xin FU

    2009-01-01

    The meiotic behavior of 10 taxa (nine species and one variety) of the genus Pinus was investigated using pollen mother cells (PMCs) to reveal the differentiation among karyotypes. Chromosome spreads were prepared by conventional squashing. The meiotic index and the average configuration were higher, whereas the frequency of aberrance (chromosomal bridges, fragments, or micronuclei) was lower, in all l0 taxa compared with other gymnosperms. The meiotic index, average configuration, and frequency of irregularity were found to be uniform among the species. It was shown that the genomes of the Pinus species investigated were highly stable, confirming results of previous mitotic analyses in this genus. However, slight differentiation of homologous chromosomes among genomes was revealed by analysis of meiotic configurations in Pinus nigra var. poiretiana. Quadrivalents were observed in 9.31% of PMCs in this species. This is the first time that quadrivalents have been observed in gynmosperms.

  10. Chemical properties of tannic extracts from bark of Pinus oocarpa and their use as adhesive

    Directory of Open Access Journals (Sweden)

    Michel Cardoso Vieira

    2014-03-01

    Full Text Available This work evaluated the properties of aqueous extracts obtained from the bark of Pinus oocarpa under addition of sodium sulfite and sodium bisulfite and the possibility of employment of tannins from the bark as an adhesive for bonding wood. After evaluation of the chemical properties of tannic extracts it was decided to employ the extraction with distilled water under addition of / 5% sodium sulfite to prepare for the tannin-formaldehyde adhesive. Adhesive phenol formaldehyde and urea-formaldehyde were modified with 10% tannin Pinus oocarpa and the effect of this addition on the quality of the adhesive was evaluated. The addition from the bark of Pinus oocarpa showed that it is possible to use pure tannin as an adhesive because of its good gluing characteristics. The addition of tannic extract to synthetic adhesives contributed to increase viscosity values. Thus the substitution of synthetic adhesives by tannins is possible only up to 10%.

  11. Modeling seed dispersal distances: implications for transgenic Pinus taeda.

    Science.gov (United States)

    Williams, Claire G; LaDeau, Shannon L; Oren, Ram; Katul, Gabriel G

    2006-02-01

    Predicting forest-tree seed dispersal across a landscape is useful for estimating gene flow from genetically engineered (GE) or transgenic trees. The question of biocontainment has yet to be resolved, although field-trial permits for transgenic forest trees are on the rise. Most current field trials in the United States occur in the Southeast where Pinus taeda L., an indigenous species, is the major timber commodity. Seed dispersal distances were simulated using a model where the major determinants were: (1) forest canopy height at seed release, (2) terminal velocity of the seeds, (3) absolute seed release, and (4) turbulent-flow statistics, all of which were measured or determined within a P. taeda plantation established from seeds collected from wild forest-tree stands at the Duke Forest near Durham, North Carolina, USA. In plantations aged 16 and 25 years our model results showed that most of the seeds fell within local-neighborhood dispersal distances, with estimates ranging from 0.05 to 0.14 km from the source. A fraction of seeds was uplifted above the forest canopy and moved via the long-distance dispersal (LDD) process as far as 11.9-33.7 km. Out of 10(5) seeds produced per hectare per year, roughly 440 seeds were predicted to be uplifted by vertical eddies above the forest canopy and transported via LDD. Of these, 70 seeds/ha traveled distances in excess of 1 km from the source, a distance too great to serve as a biocontainment zone. The probability of LDD occurrence of transgenic conifer seeds at distances exceeding 1 km approached 100%.

  12. Cytogenetic variability in pinus sylvestris L. populations experiencing anthropogenic influence

    Energy Technology Data Exchange (ETDEWEB)

    Oudalova, A.; Geras' kin, S.; Vasiliev, D.; Dikarev, V. [Russian Institute of Agricultural Radiology and Agroecology, Obninsk (Russian Federation)

    2004-07-01

    Techno-genic pollution has become one of the most significant ecological factors determining biosphere existence and development. An analysis of genetic consequences of the radiation accidents in the South Urals and Chernobyl has shown that mutation and recombination processes are considerably accelerated in plant and animal's populations experiencing techno-genic influence. This implies that there are complicated adaptation processes leading to changes in genetic structure of populations and increasing genetic load. Pinus sylvestris L. populations growing at the territory of the 'radon' Leningrad regional radioactive waste reprocessing enterprise and Sosnovy Bor town were monitored 6 years (1997-2002) by a set of cyto-genetical and morphological tests. Cytogenetic damage levels within intercalary meristem of needle as well as in root meristem of seedlings were found to significantly exceed corresponding controls. A higher radioresistance of the Scots pine seeds analyzed was demonstrated with an acute {gamma}-radiation that also revealed a selection process directed at an enhancement of repair efficiency and resulting in a shift of mean values of radioresistance in populations towards higher values. An enlargement of variance of studied cytogenetic parameters was found in the populations experiencing techno-genic influence. This indicates, with an account of phenomenon of the enhanced radioresistance, that there are processes of cyto-genetical adaptation in the investigated regions. An analysis of the structure of ecological-genetical variability was carried out with the purpose of separating two components in the inter-population variability - the first is engaged to the genetically determined variability of biological characteristics intrinsic for this species, and the second is responsible for the variability originating from anthropogenic contamination of the natural habitat. Changes of these two types of variability were studied in dependence on

  13. Rapid changes of induced volatile organic compounds in Pinus massoniana

    Institute of Scientific and Technical Information of China (English)

    REN Qin; JIN Youju; HU Yongiian; CHEN Huajun; LI Zhenyu

    2007-01-01

    Using the thermal-desorption cold trap gas chromatography/mass spectrometer(TCT-GC-MS)technique,the composition and relative contents of volatile compounds were analyzed in undamaged(control),insect-damaged(ID)and artificially-damaged(AD)leaves ofPinus massoniana in field at different times and levels of damage.Results showed that although volatile substances were highly released earlier in AD leaves plants,they were significantly less abundant in AD than in ID leaves treatments.Also,the damage level considerably influenced the changes of induced volatile products from leaves.Compared with the control,the emission rate of camphene,β-pinene,phellandrene,caryophyllene and(E)farnesene was high after 1 h in 25%-40% ID-affected leaves,whereas that of tricyclene,myrcene,camphene,β-Pinene,phellandrene and caryophyllene reached its maximum after 24 h in 60%-75% D-affected leaves.In the same manner,some volatile compounds in the AD leaves treatment displayed their peaks just after 1 h,but others after 24 h.The AD and ID leaves at the damage level of 25%-40% did not exhibit an obvious regularity with time;however,in 60%- 75% AD leaves,peaks of volatile substances were attained after 1 or 2 h.Our results also showed that the relative content ofβ-pinene increased and was higher in damaged than control plants,β-pinene plays an important role in inducing the insect resistance of P.massoniana trees.

  14. Stem compression reversibly reduces phloem transport in Pinus sylvestris trees.

    Science.gov (United States)

    Henriksson, Nils; Tarvainen, Lasse; Lim, Hyungwoo; Tor-Ngern, Pantana; Palmroth, Sari; Oren, Ram; Marshall, John; Näsholm, Torgny

    2015-10-01

    Manipulating tree belowground carbon (C) transport enables investigation of the ecological and physiological roles of tree roots and their associated mycorrhizal fungi, as well as a range of other soil organisms and processes. Girdling remains the most reliable method for manipulating this flux and it has been used in numerous studies. However, girdling is destructive and irreversible. Belowground C transport is mediated by phloem tissue, pressurized through the high osmotic potential resulting from its high content of soluble sugars. We speculated that phloem transport may be reversibly blocked through the application of an external pressure on tree stems. Thus, we here introduce a technique based on compression of the phloem, which interrupts belowground flow of assimilates, but allows trees to recover when the external pressure is removed. Metal clamps were wrapped around the stems and tightened to achieve a pressure theoretically sufficient to collapse the phloem tissue, thereby aiming to block transport. The compression's performance was tested in two field experiments: a (13)C canopy labelling study conducted on small Scots pine (Pinus sylvestris L.) trees [2-3 m tall, 3-7 cm diameter at breast height (DBH)] and a larger study involving mature pines (∼15 m tall, 15-25 cm DBH) where stem respiration, phloem and root carbohydrate contents, and soil CO2 efflux were measured. The compression's effectiveness was demonstrated by the successful blockage of (13)C transport. Stem compression doubled stem respiration above treatment, reduced soil CO2 efflux by 34% and reduced phloem sucrose content by 50% compared with control trees. Stem respiration and soil CO2 efflux returned to normal within 3 weeks after pressure release, and (13)C labelling revealed recovery of phloem function the following year. Thus, we show that belowground phloem C transport can be reduced by compression, and we also demonstrate that trees recover after treatment, resuming C

  15. DENSITY MANAGEMENT DIAGRAM OF Pinus taeda L. UNTHINNED STAND

    Directory of Open Access Journals (Sweden)

    Paulo Sérgio Pigatto Schneider

    2010-03-01

    Full Text Available This work was developed with the purpose of studying the efficiency of the models which express the density-diameter relationship in stands of Pinus taeda L., implanted in several spacing and handled in unthinning density, and were measured every year until the of 18 age. Results indicated that all of the size-density relationship of Tang model presented good statistical precision, was more efficient than the others, presenting excellent coefficient of determination (0.99, low standard error of estimate (0.0948, low coefficient of variation (1.17%, low bias (0.0086 and high efficiency (0.8976. The slope of the size-density didn’t allow proving the universality of the self-tinning law, with value of this slope is equal -3/2. With help of the size-density model, 6 Indexes of Density of the Stands (IDPs were generated, with variation from the maximum 1400 to the minimum 600, with interval of class of IDP equal to 200, having as reference the standard diameter of 25 cm. The population’s Density Management Diagram (DMD, elaborated for the dendrometric variables – average diameter, basal area and volume for hectare (by IDP – presented good efficiency with low mistake in the estimate of the values of these variables. Real volume and estimate volume per hectare in DMD presented an absolute difference of just -7.39 m3ha-1 and a relative difference of -1.79 %, showing a great precision of the DMD model. The estimates volume of DMD per hectare, in relation to the real values, presented a value of efficiency equal to 0.99, what indicates a high precision, and an  value equal to 0.00034, not significant to 0.01% of probability, demonstrating that the real and the estimate values don't differ statistical amongst themselves. 

  16. Paternity and gene dispersal in limber pine (Pinus flexilis James).

    Science.gov (United States)

    Schuster, W S; Mitton, J B

    2000-03-01

    This study provides empirical information on intrapopulation gene dispersal via pollen, the size of genetic 'neighbourhoods', and interpopulation gene flow in a long-lived conifer, limber pine (Pinus flexilis). We used allozyme loci for a paternity analysis of 518 seeds produced in an isolated population of limber pine located in north-eastern Colorado, U.S.A., separated by 2 km from the nearest conspecific trees and nearly 100 km from populations in the Rocky Mountains. We also used indirect techniques (FST analyses) to estimate gene flow rates among subdivisions of the study population and among five widely separated populations. Within the main study population limber pine exhibited a polymorphism level of 50%, observed heterozygosity of 0.159, and 2.36 alleles per polymorphic locus. Mountain populations were slightly more variable. The main study population showed significant differentiation in allozymes among neighbouring subpopulations. The mean FST was 0.031 and the gene flow rate among subpopulations was estimated as 7.8 migrants per generation. Among widely separated populations the mean FST was 0.035 and the gene flow rate was estimated as 6.9 migrants per generation. The paternity analysis indicated a best estimate of 6.5% pollen immigration (minimum 1.1%) from populations 2 km to 100+ km away. For 4% of the seeds examined, paternity could be ascribed to a single tree in the study population. Fractional paternity and likelihood methods were used to estimate pollen dispersal distances for the remainder of the seeds. Mean pollen dispersal distance was estimated at 140 m using the fractional method, similar to results from the other techniques. This compares with a mean distance of 172 m between potential mates. These results suggest near-panmictic pollen dispersal over this population, which covers about 15 ha. The observed allozyme differences and surprisingly low estimates of among-subpopulation gene flow are ascribed to a probable restriction of gene

  17. Biomass production of young lodgepole pine (Pinus contorta var. latifolia stands in Latvia

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    Jansons A

    2013-01-01

    Full Text Available Biomass as a source of renewable energy is gaining an increasing importance in the context of emission targets set by the European Union. Large areas of abandoned agricultural land with different soils are potentially available for establishment of biomass plantations in the Baltic states. Considering soil and climatic requirements as well as traits characteristic for lodgepole pine (Pinus contorta Dougl. var. latifolia Engelm and the scarcity of published knowledge, we assessed the above-ground biomass of Pinus contorta in comparison to that of native Scots pine (Pinus sylvestris L. and factors affecting biomass production. Data were collected in 3 experimental trials, located in two sites in central part of Latvia: Zvirgzde and Kuldiga (56°41’ N, 24°28’ E and 57°03’ N, 21°57’ E, respectively. Trials were established with density 5000 tree ha-1, using seed material from Canada (50°08’-60°15’ N, 116°25’-132°50’ W and two Pinus contorta stands with unknown origin growing in Latvia. Results reveal that absolute dry aboveground biomass of Pinus contorta reaches 114 ± 6.4 t ha-1 at age 16 on a fertile former arable land, 48 ± 3.6 and 94 ± 9.4 t ha-1 at age 22 and 25, respectively, on a sandy forest land (Vacciniosa forest type. The biomass is significantly (p < 0.01 and considerably (more than two-fold higher than that of the native Pinus sylvestris and the productivity is similar (in fertile soils or higher (on poor soils than reported for other species in energy-wood plantations. Provenance was a significant factor affecting the above-ground biomass, and the ranking of provenances did not change significantly between different soil conditions. It provides opportunities for further improvement of productivity using selection.

  18. Organization of the endoplasmic reticulum in dividing cells of the gymnosperms Pinus brutia and Pinus nigra, and of the pterophyte Asplenium nidus.

    Science.gov (United States)

    Zachariadis, M; Quader, H; Galatis, B; Apostolakos, P

    2003-01-01

    Endoplasmic reticulum (ER) organization in the dividing cells of the pterophyte Asplenium nidus and of the gymnosperms Pinus brutia and Pinus nigra has been studied by immunolocalization techniques using the monoclonal antibody 2E7, which recognizes luminar ER resident proteins containing C-terminal HDEL sequences. In the pterophyte, the ER reorganization during cell cycle is similar to that in angiosperms. Among others, prominent ER gatherings were found at the mitotic spindle poles and in the phragmoplast during cytokinesis. However, in the gymnosperms examined, the ER displays a unique pattern of reorganization not described so far. In both the Pinus species, well-defined ER patterns are successively formed during cell cycle. They are the preprophase ER-band, the prophase- metaphase- and anaphase ER-spindle, the interzonal ER-system, the ER-phragmoplast and an ER-system lining the daughter cell wall. The ER patterns are closely similar to that of the correspondent microtubule (MT) arrangements with which they are co-organized. Observations made on P. nigra root-cells affected by oryzalin, colchicine and cytochalasin D favour the conclusion that the pattern of ER organization is controlled during mitosis and cytokinesis by the MT cytoskeleton.

  19. Seed source variation and conservation of Pinus wallichiana in India

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    M. Thapliyal

    2008-09-01

    Full Text Available Pinus wallichiana A.B. Jacks is an important component of the middleand high altitude Himalayan forests having large natural distribution ranging between 260 to 360 N latitude and 690 to 750 E longitudes. It is commonly known as Himalayan blue pine or blue pine, being indigenous to Himalayan Mountain regions and because of its bluish or grayish-green leaves. It is a five needle pine which gained world-wide attention for its resistance to blister rust among white pines. This species has been crossed successfully with other white pines and vigorous hybrids have been obtained. Considerable variation in morphological and anatomical characteristics of needles, cones and seeds in natural stands exists across the natural distribution of the species, especially in mesic and xeric habitats. These variationssuggested the differentiation of this species in ecotypes or varieties as reported by various authors. However, the level of genetic diversity was found to be relatively high and the degree of genetic differentiation was low compared to other pines. Thewide range of climatic conditions in the natural distribution of this pine is expected to result in high genetic variation within different populations of the species. The study aims to determine the nature and extent of variation present in the populations of the species in respect to cone and seed characteristics across its natural distribution. Seed of 17 seed sources from the states of Uttaranchal and Himachal Pradesh was collected and analyzed for cone characters (fresh weight of cones, cone length, cone width, specific gravity and seed characters (seed weight, moisture content, germination percent, cotyledon number. Significant variations have beenobserved in these traits among different seed sources of the species. The cone weight varied from 44.4 to114g and the higher cone weight was recorded at higher altitudes.The germination percent varied from 40 to 85 whereas cotyledon number varied from 7

  20. Seed source variation and conservation of Pinus wallichiana in India

    Directory of Open Access Journals (Sweden)

    Manisha Thapliyal

    2013-12-01

    Full Text Available Pinus wallichiana A.B. Jacks is an important component of the middle and high altitude Himalayan forests having large natural distribution ranging between 260 to 360 N latitude and 690 to 750 E longitudes. It is commonly known as Himalayan blue pine or blue pine, being indigenous to Himalayan Mountain regions and because of its bluish or grayish-green leaves. It is a five needle pine which gained world-wide attention for its resistance to blister rust among white pines. This species has been crossed successfully with other white pines and vigorous hybrids have been obtained. Considerable variation in morphological and anatomical characteristics of needles, cones and seeds in natural stands exists across the natural distribution of the species, especially in mesic and xeric habitats. These variations suggested the differentiation of this species in ecotypes or varieties as reported by various authors. However, the level of genetic diversity was found to be relatively high and the degree of genetic differentiation was low compared to other pines. The wide range of climatic conditions in the natural distribution of this pine is expected to result in high genetic variation within different populations of the species. The study aims to determine the nature and extent of variation present in the populations of the species in respect to cone and seed characteristics across its natural distribution. Seed of 17 seed sources from the states of Uttaranchal and Himachal Pradesh was collected and analyzed for cone characters (fresh weight of cones, cone length, cone width, specific gravity and seed characters (seed weight, moisture content, germination percent, cotyledon number. Significant variations have been observed in these traits among different seed sources of the species. The cone weight varied from 44.4 to114g and the higher cone weight was recorded at higher altitudes. The germination percent varied from 40 to 85 whereas cotyledon number varied

  1. Micropropagation of an endangered species Pinus armandii var. Amamiana

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    Katsuaki Ishii

    2013-12-01

    Full Text Available For micropropagation via organ culture, mature embryos were excised from the seeds of Pinus armandii. Franch. var. amamiana (Koidz. Hatusima, an endangered species only inhabiting the south west islands of Japan. Adventitious buds were induced on the surface of the embryo on 1/2 DCR medium containing BAP, and they grew shoots after subculturing to medium containing activated charcoal or a low concentration of thidiazuron. From the elongated shoots, root primordia and roots were induced in medium containing IBA as an auxine. We found that a low concentration of zeatin or BAP added to the medium was beneficial for plant regeneration of mature embryos of this species. For micropropagation via somatic embryogenesis, embryogenic cell suspensions were induced from a mature and immature seed of P. armandii var. amamiana on MS liquid medium supplemented with 1 ľM 2, 4-D and 3 ľM BAP. The suspensions were incubated in the dark at 250. Induced suspension cells were transferred to ammonium free MS liquid medium supplemented with 1 ľM 2, 4-D, 3 ľM BAP and 30m M L-glutamine and subcultured every 2 weeks. In the other set of the experiment, the induction rate of somatic embryogenesis was high with ammonium free half strength MS medium. In order to develop somatic embryos, the suspension cells were transferred to ammonium free MS medium supplemented with 10 ľM ABA, 0.2% activated charcoal, 10% PEG (MW6000, 30m M L-glutamine and 6% maltose. The cultures were incubated under a 16h light/8h dark photoperiod. After 1-2 months of culture, differentiation of embryos progressed and cotyledonary embryos were obtained. These embryos were transferred on ammonium free MS solid medium under 16 h photoperiod. After 2-3 weeks plantlets with roots and green cotyledons were obtained. Plantlets were transplanted to vermiculite containing modified MS liquid medium in 200 ml culture flasks, then out planted after habituation procedure.

  2. Mesohabitats, fish assemblage composition, and mesohabitat use of the Rio Grande silvery minnow over a range of seasonal flow regimes in the Rio Grande/Rio Bravo del Norte, in and near Big Bend National Park, Texas, 2010-11

    Science.gov (United States)

    Moring, J. Bruce; Braun, Christopher L.; Pearson, Daniel K.

    2014-01-01

    In 2010–11, the U.S. Geological Survey (USGS), in cooperation with the U.S. Fish and Wildlife Service, evaluated the physical characteristics and fish assemblage composition of mapped river mesohabitats at four sites on the Rio Grande/Rio Bravo del Norte (hereinafter Rio Grande) in and near Big Bend National Park, Texas. The four sites used for the river habitat study were colocated with sites where the U.S. Fish and Wildlife Service has implemented an experimental reintroduction of the Rio Grande silvery minnow (Hybognathus amarus), a federally listed endangered species, into part of the historical range of this species. The four sites from upstream to downstream are USGS station 08374340 Rio Grande at Contrabando Canyon near Lajitas, Tex. (hereinafter the Contrabando site), USGS station 290956103363600 Rio Grande at Santa Elena Canyon, Big Bend National Park, Tex. (hereinafter the Santa Elena site), USGS station 291046102573900 Rio Grande near Ranger Station at Rio Grande Village, Tex. (hereinafter the Rio Grande Village site), and USGS station 292354102491100 Rio Grande above Stillwell Crossing near Big Bend National Park, Tex. (hereinafter the Stillwell Crossing site).

  3. Penafsiran Volume Pohon Pinus merkusii melalui Foto Udara (Studi Kasus BKPH Majenang, KPH Banyumas Barat

    Directory of Open Access Journals (Sweden)

    Sahid -

    2013-01-01

    Full Text Available Interpretation of  Stock Volume of Pinus merkusii  using Aerial Photographs (Case Study in BKPH Majenang, West Banyumas FMU The study aims to compile and analyse the growing stock volume of Pinus merkusii by using stand parameter measured through aerial photographs. The study was conducted by using black-white panchromatic aerial photographs produced in 2005 and the scale was 1 : 20,000. The study was conducted in Pinus merkusii taping plots in the Majenang BKPH, under the forest districts of west Banyumas, Perum Perhutani using tree height, crown diameter and number of trees per hectare as parameters. Data analysis was done to define the relationship among these parameters in regression equation. The steps of the study were defining measurement plots in aerial photographs, measurement of stand parameters in the aerial photographs and field observation. Data analysis was performed using micro computer. As a result, the regression equation for each location as follows: V = 72,415 – 0,231 N + 35,242 H – 24,454D – 0,923 (N x D, with the determination coefficient was 0,789 and the significance of 95%. Where: V = growing stock volume (m3, N = the number of trees per hectare, H = tree height (m and D = crown diameter (m. It was concluded that number of tree per hectare, tree height and crown diameter were good predictors for standing stock volume of Pinus merkusii.

  4. Studies on Ectomycorrhizae—XV. Mycorrhizae formed by Rhizopogon luteolus on Pinus silvestris

    NARCIS (Netherlands)

    Uhl, M.

    1988-01-01

    The mycorrhizae of Rhizopogon luteolus on Pinus silvestris are comprehensively described. They are characterized by a coralloid habit, ochre to yellow colour, highly differentiated rhizomorphs, a two-layered mantle and a differentiated autofluorescence in mantle, Hartig net and rhizomorphs. The lite

  5. Fertility variation and status number in clonal seed orchards of Pinus sylvestris.

    Science.gov (United States)

    Bilir, Nebi; Temiraga, Halime

    2012-11-15

    The present study was carried out to evaluate fertility variation, status number and gene diversity based on strobili productions in two clonal seed orchards of Scots pine (Pinus sylvestris L.). There were large differences among clones for the female and male strobili productions in the orchards. Positive and significant (pseed equally from clones or genetinc thinning.

  6. Reproduction ecology of Pinus halepensis : a monoecious, wind-pollinated and partially serotinous Mediterranean pine tree

    NARCIS (Netherlands)

    Goubitz, Shirrinka

    2002-01-01

    Fire is an important factor in the evolution and ecology of Mediterranean plant species. The fire frequency has increased in the 20st century. Pines are the most important tree species in the area. Pinus halepensis is the only natural pine in parts of the east Mediterranean basin, such as Israel and

  7. [Genetic control of the isoenzymes in Cembra pine (Pinus cembra L.) in the Ukrainian Carpathian Mountains].

    Science.gov (United States)

    Pirko, Ia V; Korshikov, I I

    2001-01-01

    Genetic control of GOT, GDH, DIA, MDH, SOD, FDH, ADH, ACP, and LAP enzymes was studied in the seed megagametophytes of cembra pine (Pinus cembra L.) from the natural population of the Ukrainian Carpa-thian mountains. Efficient electrophoretic separation was obtained for 21 loci products. The analysis of allele segregation in heterozygous trees confirms monogenic inheritance of the revealed variants.

  8. EFFECT OF ATMOSPHERIC AMMONIA ON THE NITROGEN-METABOLISM OF SCOTS PINE (PINUS-SYLVESTRIS) NEEDLES

    NARCIS (Netherlands)

    PEREZSOBA, M; STULEN, [No Value; VANDEREERDEN, LJM

    1994-01-01

    Four-year-old seedlings of Scots pine (Pinus sylvestris L.) were exposed to filtered air (FA), and to FA supplemented with NH3 (60 and 240 mu g m(-3)) in controlled-environment chambers for 14 weeks. Exposure to the higher NH, concentration resulted in an increased activity of glutamine synthetase (

  9. Multi-Season Monoterpene and Sesquiterpene Analysis of Pinus taeda Needle Tissue

    Science.gov (United States)

    Pinus taeda (Loblolly pine) is one of the worlds most important timber crop and accounts for a significant portion of the southeastern U.S. landcover. Biogenic voltile organic compound (BVOC) content was extracted from the tissue material of P. taeda needles and analyzed over a m...

  10. Fungal species-specific responses of ectomycorrhizal Scots pine (Pinus sylvestris to elevated (CO2)

    NARCIS (Netherlands)

    Gorissen, A.; Kuyper, T.W.

    2000-01-01

    Ectomycorrhizal seedlings of Scots pine (Pinus sylvestris) inoculated with the nitrotolerant Laccaria bicolor and the nitrophobic Suillus bovinus were exposed to ambient (350 l l1) and elevated (700 l l1) [CO2]. After 79 d the seedlings were labelled for 28 d with 14CO2, after which they were harves

  11. Two new sesquiterpene glycosides isolated from the fresh needles of Pinus massoniana Lamb.

    Science.gov (United States)

    Xiao, Yun-Chuan; Ye, Liang; Zhao, Man-Xi; Yan, Cui-Qi; Wang, Wei; Huang, Quan-Shu; Liang, Kai; Meng, Bao-Hua; Ke, Xiao

    2017-02-01

    Two new sesquiterpene glycosides, namely massonside A (1) and massonside B (2), were isolated from the n-Bu extract of the fresh needles of Pinus massoniana Lamb. Their structures were established by 1D, 2D nuclear magnetic resonance and high-resolution mass spectrometry. Their biological activities were profiled by the anti-HBV and anti-HCV assays.

  12. Grafting Techniques of Pinus densiflora var. Zhangwuensis%彰武松嫁接技术

    Institute of Scientific and Technical Information of China (English)

    叶晓梅

    2012-01-01

    通过对樟子松实生苗及其与彰武松的嫁接苗生长对照,研究了樟子松嫁接彰武松的可行性,表明樟子松嫁接彰武松成活率平均为77.3%,嫁接苗在嫁接后1a、2a高度生长分别高于樟子松实生苗36.0%和44.0%,在嫁接后9a,高度和地径生长分别高于樟子松82.0%和47.0%,以樟子松为砧木嫁接彰武松可以在北方推广。%Seeded plant of Pinus sylvestnis var. mongolica Litv. were compared with the grafted seed- ling with Pinus densiflora var. Zhangwuensis in terms of their growth. The result showed that the grafted seedlings would reach 77.3%, the height growth at 1 a and 2 a is 36.0% and 44.0% over the seedlings of Pinus sylvestnis var. mongolica Litv. After 9 years, the height growth and stem growth were 82.0% and 47.0% over, indicating the seedlings with Pinus sylvestnis var. mongolica Litv. as the stock is suitable for north China,

  13. Draft Genome Sequence of Phytopathogenic Fungus Fusarium fujikuroi CF-295141, Isolated from Pinus sylvestris

    Science.gov (United States)

    Bertoni-Mann, Michele; Sánchez-Hidalgo, Marina; González-Menéndez, Victor

    2016-01-01

    Here, we report the draft genome sequence of a new strain of Fusarium fujikuroi, isolated from Pinus sylvestris, which was also found to produce the mycotoxin beauvericin. The Illumina-based sequence analysis revealed an approximate genome size of 44.2 Mbp, containing 164 secondary metabolite biosynthetic clusters. PMID:27795279

  14. WATER-USE ALONG A HYDROLOGICAL GRADIENT IN CENTRAL FLORIDA: A TALE OF TWO PINUS SPECIES

    Science.gov (United States)

    Although central Florida is relatively flat, the distribution of species on the landscape is controlled by subtle changes in elevation. Along a four-meter elevation gradient, xeric sandhill vegetation dominated by Pinus palustris (Longleaf pine) gives way to mesic pine flatwoods...

  15. Pycnogenol

    Science.gov (United States)

    Pycnogenol is the US registered trademark name for a product derived from the pine bark of a ... known as Pinus pinaster. The active ingredients in pycnogenol can also be extracted from other sources, including ...

  16. Volume de madeira de Pinus taeda L. em diferentes espaços vitais de crescimento. Wood volume of Pinus taeda L. at different growing spacings.

    Directory of Open Access Journals (Sweden)

    Rodrigo LIMA

    2015-12-01

    Full Text Available Pinus taeda L. é uma das espécies do gênero Pinus mais plantadas na região Sul do Brasil por apresentar excelente crescimento e ótima adaptação às condições climáticas e de solo. Essa espécie é utilizada em larga escala, principalmente para a produção de celulose, construção civil, laminação, produção de móveis, particulados e serraria. Objetivou-se avaliar a produção volumétrica de Pinus taeda L. em diferentes espaços vitais de crescimento (entre 1 m2 e 16 m2 por planta propiciados por nove diferentes espaçamentos entre árvores de um ensaio em cinco blocos ao acaso. O trabalho baseou-se nas medidas de altura e DAP em 25 árvores internas da parcela, aos sete anos após plantio das mudas oriundas de pomar de sementes clonal. Valores estimados de volume por hectare foram inversamente proporcionais ao aumento do espaço vital, alcançando entre 74,2 e 274,8 m3 /ha. Os incrementos médios em volume atingiram entre 10,60 e 39,25 m3 /ha/ano. Concluiu-se que, se o objetivo é a produção volumétrica mesmo com diâmetros pequenos, deve-se optar por espaços vitais menores. Quando se deseja maiores diâmetros, a opção é por espaços maiores. No presente caso, o melhor compromisso entre produção volumétrica e diâmetros grandes pode estar nos espaços vitais intermediários, entre 5 e 8 m2 para cada árvore. Pinus taeda L. is one of the most Pinus species planted in southern Brazil, because it presents excellent growth and optimum adaptation to climatic and soil condition. The species is used in large scale, mainly for cellulose production, construction, laminating, production of furniture, particulates and sawmill. It was aimed to evaluate the Pinus taeda L. volumetric production at different growth vital spaces (ranging 1 m² and 16 m² per plant provided by nine different spacings between plants, the trial was installed in a randomized blocks with five replications. The study was based on height and DBH measurements

  17. 568 Prevalence of Sensitization to Parietaria, Pinus, Cupressus and Morus Pollens in Patients from Craic

    OpenAIRE

    Leal-Garcia, Liborio Rafael; González-Díaz, Sandra; Arias-Cruz, Alfredo; del Carmen Zarate, Maria; Garcia-Calderin, Diego; Gallego, Claudia

    2012-01-01

    Background The pollens of Cupressus, Parietaria, Pinus and Morus are important causes of allergic respiratory diseases. In a study of pollen concentration in Monterrey in 2004, these pollens occupied the first places in frequency. The objective was to determine the prevalence of sensitization to Parietaria, Pinus, Cupressus and Morus in patients from CRAIC (Regional Center of allergy and clinical immunology, Monterrey, México) Methods Is an observational, cross-comparative, double-blind study...

  18. Identification case of evidence in timber tracing of Pinus radiate, using high-resolution melting (HRM) analysis.

    Science.gov (United States)

    Solano, Jaime; Anabalón, Leonardo; Encina, Francisco

    2016-03-01

    Fast, accurate detection of plant species and their hybrids using molecular tools will facilitate assessment and monitoring of timber tracing evidence. In this study the origin of unknown pine samples is determined for a case of timber theft in the region of Araucania southern Chile. We evaluate the utility of the trnL marker region for species identification applied to pine wood based on High Resolution Melting. This efficient tracing methods can be incorporated into forestry applications such as certification of origin. The object of this work was genotype identification using high-resolution melting (HRM) and trnL approaches for Pinus radiata (Don) in timber tracing evidence. Our results indicate that trnL is a very sensitive marker for delimiting species and HRM analysis was used successfully for genotyping Pinus samples for timber tracing purposes. Genotyping samples by HRM analysis with the trnL1 approach allowed us to differentiate two wood samples from the Pinaceae family: Pinus radiata (Don) and Pseudotsuga menziesii (Mirb.) Franco. The same approach with Pinus trnL wood was not able to discriminate between samples of Pinus radiata, indicating that the samples were genetically indistinguishable, possibly because they have the same genotype at this locus. Timber tracing with HRM analysis is expected to contribute to future forest certification schemes, control of illegal trading, and molecular traceability of Pinus spp.

  19. Evaluación de Pinus pseudostrobus Lindl. y Pinus greggii Engelm. con dos densidades de plantación en Michoacán, México

    OpenAIRE

    H. Jesús Muñoz Flores; Gabriela Orozco Gutierrez; Víctor M. Coria Avalos; J. Jesús García Sánchez; Yadira Yolanda Muñoz Vega; Gabriel Salvador Cruz

    2011-01-01

    En la región Purhépecha, del estado de Michoacán, los problemas ambientales son la deforestación, disminución de la biodiversidad, erosión de suelos, agotamiento de manantiales, contaminación por agroquímicos, cambio de uso de suelo y fragmentación de bosques; y para contrarrestar estos efectos se han establecido plantaciones desde 1960 a la fecha. El objetivo fue evaluar el desarrollo en altura, diámetro, supervivencia y el estado fitosanitario de la plantación de Pinus pseudostrobus Lindl.,...

  20. Estimation of hydraulic characteristics in the Santa Fe Group aquifer system using computer simulations of river and drain pulses in the Rio Bravo study area, near Albuquerque, New Mexico

    Science.gov (United States)

    Roark, D.M.

    2001-01-01

    In 1997, the U.S. Geological Survey conducted a hydrologic investigation of the surface-water/ground-water interaction of the Rio Grande and the surrounding alluvium and the Santa Fe Group aquifer system in an area near the Rio Bravo Bridge, south of Albu- querque, New Mexico. A set of existing wells and new wells were instrumented to monitor water levels in a section perpendicular to the Rio Grande on the east side of the river. Equipment to measure stream stage was installed at two sites--on the Albuquerque Riverside Drain and on the Rio Grande. A short-duration river pulse and a long-duration river pulse were used to stress the ground-water system while the changes in water levels were monitored. A ground- water flow-model simulation using the principle of superposition was used to estimate the hydraulic characteristics of the local ground-water system. Simulated horizontal hydraulic conductivities varied from 0.03 to 100 feet per day, and vertical hydraulic conductivities varied from 1.5 x 10-6 to 0.01 foot per day. The specific yield of layer 1 was estimated to be 0.3. Specific storage for layers 2 through 11 was 1.0 x 10-6. Water entering the model from the river along a 300-foot-wide cross section during simulation of the short-duration pulse averaged 7.46 x 10-3 cubic foot per second and during the long-duration pulse was 1.66 x 10-3 cubic foot per second. The average flux from the model to the drain during the short-duration pulse was 3.18 x 10-3 cubic foot per second. The average flux for the long-duration pulse was 7.14 x 10-3 cubic foot per second from the drain to the model.

  1. Characterization of polymorphic microsatellite markers in Pinus armandii (Pinaceae), an endemic conifer species to China1

    Science.gov (United States)

    Dong, Wan-Lin; Wang, Ruo-Nan; Yan, Xiao-Hao; Niu, Chuan; Gong, Lin-Lin; Li, Zhong-Hu

    2016-01-01

    Premise of the study: Pinus armandii (Pinaceae) is an important conifer tree species in central and southwestern China, and it plays a key role in the local forest ecosystems. To investigate its population genetics and design effective conservation strategies, we characterized 18 polymorphic microsatellite markers for this species. Methods and Results: Eighteen novel polymorphic and 16 monomorphic microsatellite loci of P. armandii were isolated using Illumina MiSeq technology. The number of alleles per locus ranged from two to five. The expected heterozygosity ranged from 0.061 to 0.609 with an average of 0.384, and the observed heterozygosity ranged from 0.063 to 0.947 with an average of 0.436. Seventeen loci could be successfully transferred to five related Pinus species (P. koraiensis, P. griffithii, P. sibirica, P. pumila, and P. bungeana). Conclusions: These novel microsatellites could potentially be used to investigate the population genetics of P. armandii and related species.

  2. Discussion on Pipe Model through Hydraulic Architecture of Pinus tabulaeformis Seedling

    Institute of Scientific and Technical Information of China (English)

    ZHAIHongbo; LIJiyue; NIELishui

    2004-01-01

    In the paper, the hydraulic architecture parameters of Pinus tabulaeformis seedlings (4 years old) were measured by improved flushing method under normal water condition in the green house and the basic theory of hydraulic architecture is used to discuss the rationality of the pipe model. The results of the experiment and simulation show that the differences of hydraulic conductivity, specific conductivity and leaf specific conductivity is great in different stems and branches of Pinus tabulaeformis seedlings. The hydraulic conductivity of non-constriction area is higher than that of constriction area. The devotion of functional xylem of stem to unit leaf growth is not a constant, namely, the Huber value is diverse. Even though the pipe model has been accepted in some areas, its precondition is not perfect, and it is helpless in correctly understanding the essence of water transport in seedlings from the prospective of water physiology.

  3. Outcrossing rates in a natural stand and in a seed orchard of Pinus peuce

    Directory of Open Access Journals (Sweden)

    P. Zhelev

    2013-12-01

    Full Text Available The mating system parameters were studied in a natural stand and in a seed orchard of the Balkan endemic, Pinus peuce Griseb. bymeans of isozyme gene markers. The results indicate that the multilocus estimates of the outcrossing rates are low relative to otherwidespread conifers but concord to these reported for other pine species with limited area of distribution. No significant differenceswere detected between the natural stand and the seed orchard estimates. Multilocus estimates of outcrossing rate were within the range0.77-0.79, while mean single-locus estimates were lower (0.69-0.73. The results indicate that at least 20% of the progeny of Pinus peuceis due to self-fertilization. Possible reasons for the results observed and the implications for tree breeding and gene conservation are discussed.

  4. Radial variation of wood physical properties in Pinus patula growing in Mozambique

    Directory of Open Access Journals (Sweden)

    Claudio Gumane Francisco Juizo

    2015-09-01

    Full Text Available  This study aimed to evaluate wood physical properties of Pinus patula, aged 38, from a forest stand located in Machipanda, central region of Mozambique. Determination of physical properties, such as basic specific gravity, shrinkage, and moisture content of wood in the pith-bark followed the recommendations of the Panamerican Standard - COPANT. Results showed statistically significant differences at significance level of 5%, in the radial direction for the shrinkage coefficient in the pith-bark. Radial and tangential shrinkage values were 6.06 and 8.80% in juvenile wood and 6.18 and 8.65% in adult wood, respectively, with anisotropy coefficient of 1.43 for juvenile wood and 2.44 for adult wood. Pinus patula growing in Inhamacari forest shows stable wood at age 38. It can be used for structural purposes and for manufacture of joinery and furniture.

  5. Variability of Pinus mugo Turra clones from Ostry Wierch peat bog

    Directory of Open Access Journals (Sweden)

    Alina Bączkiewicz

    2014-01-01

    Full Text Available Present study aimed at determining genetic structure of the Pinus mugo Turra population, growing at a peat bog, on Ostry Wierch slopes in the High Tatra mountains, in respect to the number of genotypes present in the population, and at defining variabilities of morphological and anatomical traits. In the examined plants, isoenzymatic analysis demonstrated existence of only two genotypes (A and B, which formed relatively vast clones. Mathematical-statistical analysis of 13 anatomical - morphological traits of the needles proved that the examined clump consisted of two clearly distinguishable groups, corresponding to the two earlier electrophoretically identified clones A and B. The obtained data allowed to conclude that the method based on anatomical-morphological measurements may, at least in some cases, permit identification of Pinus mugo clones

  6. Reconstructing the plant mitochondrial genome for marker discovery: a case study using Pinus.

    Science.gov (United States)

    Donnelly, Kevin; Cottrell, Joan; Ennos, Richard A; Vendramin, Giovanni Guiseppe; A'Hara, Stuart; King, Sarah; Perry, Annika; Wachowiak, Witold; Cavers, Stephen

    2016-12-20

    Whole-genome-shotgun (WGS) sequencing of total genomic DNA was used to recover ~1 Mbp of novel mitochondrial (mtDNA) sequence from Pinus sylvestris (L.) and three members of the closely-related Pinus mugo species complex. DNA was extracted from megagametophyte tissue from six mother trees from locations across Europe and 100 bp paired-end sequencing was performed on the Illumina HiSeq platform. Candidate mtDNA sequences were identified by their size and coverage characteristics, and by comparison with published plant mitochondrial genomes. Novel variants were identified, and primers targeting these loci were trialled on a set of 28 individuals from across Europe. In total, 31 SNP loci were successfully resequenced, characterising 15 unique haplotypes. This approach offers a cost effective means of developing marker resources for mitochondrial genomes in other plant species where reference sequences are unavailable. This article is protected by copyright. All rights reserved.

  7. Volatiles as Chemosystematic Markers for Distinguishing Closely Related Species within the Pinus mugo Complex.

    Science.gov (United States)

    Celiński, Konrad; Bonikowski, Radosław; Wojnicka-Półtorak, Aleksandra; Chudzińska, Ewa; Maliński, Tomasz

    2015-08-01

    Headspace solid-phase microextraction (HS-SPME) coupled to GC/MS analysis was used to identify the constituents of pine-needle volatiles differentiating three closely-related pine species within the Pinus mugo complex, i.e., P. uncinata Ramond ex DC., P. uliginosa G.E.Neumann ex Wimm., and P. mugo Turra. Moreover, chemosystematic markers were proposed for the three analyzed pine species. The major constituents of the pine-needle volatiles were α-pinene (28.4%) and bornyl acetate (10.8%) for P. uncinata, δ-car-3-ene (21.5%) and α-pinene (16.1%) for P. uliginosa, and α-pinene (20%) and δ-car-3-ene (18.1%) for P. mugo. This study is the first report on the application of the composition of pine-needle volatiles for the reliable identification of closely-related pine species within the Pinus mugo complex.

  8. Photosynthetic acclimation to enriched CO{sub 2} concentrations in Pinus Ponderosa

    Energy Technology Data Exchange (ETDEWEB)

    Torres, M.P. [California State Univ., Humbolt, CA (United States)

    1995-11-01

    By the middle of the 21st century earth`s ambient CO{sub 2} level is expected to increase two-fold ({approximately}350 umol/L). Higher levels of CO{sub 2} are expected to cause major changes in the morphological, physiological, and biochemical traits of the world`s vegetation. Therefore, we constructed an experiment designed to measure the long-term acclimation processes of Pinus Ponderosa. As a prominent forest conifer, Pinus Ponderosa is useful when assessing a large scale global carbon budget. Eighteen genetically variable families were exposed to 3 different levels of CO{sub 2} (350 umol/L, 525 umol/L, 700 umol/L), for three years. Acclimation responses were quantified by assays of photosynthetic rate, chlorophyll fluorescence, and chlorophyll pigment concentrations.

  9. Modelling silviculture alternatives for managing Pinus pinea L. forest in North-East Spain

    Energy Technology Data Exchange (ETDEWEB)

    Piqu-Nicalau, M.; Rio, M. del; Calama, R.; Montero, G.

    2011-07-01

    A yield model was developed to simulate silviculture alternatives for Pinus pinea L. in north-east Spain (Catalonia). The model uses several functions to estimate the main silvicultural parameters at stand level and a disaggregation system to predict diameter distributions. From a network of 75 temporary plots a system of equations to predict stand variables was simultaneously fitted for two stand density types, namely low and high density stands, using the three stage least-squares method (3SLS). The diameter distributions were estimated by the Weibull distribution function using the parameter recovery method (PRM) and the method of moments. Based on this yield model, two silviculture alternatives were simulated for each stand density type and site class, resulting in 16 silviculture scenarios. The yield model and silviculture alternatives offer a management tool and a guide for the sustainable forest management of even aged Pinus pinea forests in this region. (Author) 56 refs.

  10. Niche of insect borers within Pinus massoniana infected by pine wood nematode

    Institute of Scientific and Technical Information of China (English)

    SONG Jiying; LUO Youqing; SHI Juan; YAN Xiaosu; CHEN Weiping; JIANG Ping

    2006-01-01

    In November 2003 and June 2004,the insect borers and their spatial distribution within Pinus massoniana were investigated in Zhoushan City,in East China's Zhejiang Province,where pine wood nematode (Bursaphelenchus xylophilus) are typically found.The niche width,proportional similarity of niche and the niche overlap of dominant species of dying trees were computed.Results show that five insect species infect and damage Pinus massoniana,which had been infected by pine wood nematodes,among which four are wood boring beetles and one termite.Species within host trees vary from winter to summer and all the species have their own niche width,proportional similarity of niche and the niche overlap.They can achieve competitive equilibrium and coexistence according to their biological characteristics and life habits.

  11. Family Outcrossing Rates of Pinus caribaea Morelet var. caribaea in Seed Orchard and Natural Populations

    Institute of Scientific and Technical Information of China (English)

    2002-01-01

    Family outcrossing rates of Pinus caribaea var. caribaea populations that differed in origin and management were estimated by using isozyme markers. The family outcrossing rates were estimated by (1)fixing the probability of pollen gene frequency (p); (2) allowing pollen gene frequency to vary among families. The estimates of family outcrossing rates for all the populations varied widely among families (clones of the seed orchard), ranging from 0.39 to 2.0. The average family outcrossing rates of both m...

  12. A Study on the Impacts of Climate Change on the Distribution of Pinus tabulaeformis in China

    Institute of Scientific and Technical Information of China (English)

    2002-01-01

    A special interpolation program GREEN has been developed to determine the climatic parameters for the distribution region of Chinese pine (Pinus tabulaeformis Carr.) based on the researches on the distribution of the tree species. The central region, which is the most suitable distribution region, and potential distribution region of the species under current climatic conditions were mapped using a Geographic Information System IDRISI. According to the averaged predictions by 5 GCMs for 2030, a projecti...

  13. Climatic signal from Pinus leucodermis axial resin ducts: a tree-ring time series approach

    OpenAIRE

    Antonio Saracino; Angelo Rita; Sergio Rossi; Laia Andreu-Hayles; G. Helle; Luigi Todaro

    2016-01-01

    Developing long-term chronologies of tree-ring anatomical features to evaluate climatic relationships within species might serve as an annual proxy to explore and elucidate the climatic drivers affecting xylem differentiation. Pinus leucodermis response to climate was examined by analyzing vertical xylem resin ducts in wood growing at high elevation in the Apennines of peninsular Southern Italy. Early- and latewood tree-ring resin duct chronologies, spanning the 1804–2010 time period, were co...

  14. APPLICATION OF NEUTRON RADIOGRAPHY TO INVESTIGATE CHANGES IN PERMEABILITY IN BACTERIA TREATED PINUS RADIATA TIMBER

    OpenAIRE

    Nijdam,J. J.; Lehmann, E.; Keey,R B

    2004-01-01

    The permeability of softwoods can be enhanced by selective bacterial attack of the pit membranes. In this paper, green flat-sawn Pinus radiata sapwood boards were sprinkled for various exposure times with a nutrient solution containing a mixed bacterial population. The timber samples were subsequently dried and the tangential absorption of water was measured using neutron radiography to track the movement of moisture within the wood. There was a significant increase in water absorption after ...

  15. Ophiostoma ips asociado al insecto descortezador (Dendroctonus adjunctus ) del pino de las alturas (Pinus hartwegii )

    OpenAIRE

    2009-01-01

    In the pino de las alturas (Pinus hartwegii Lindl) it has been reported the presence of the bark beetle Dendroctonus adjunctus associated with the genus Ophiostoma spp., which causes the blue-stain of the wood. The objective of this study was to identify and characterize the Ophiostoma species associated with the bark beetle D. adjunctus in P. hartwegii. Galleries and insects were collected in the Zoquiapan Experimental Forest Station (ZEFS), of the Universidad Autonoma Chapingo, in Zoquiap...

  16. Antifungal metabolites from fungal endophytes of Pinus strobus

    DEFF Research Database (Denmark)

    Sumarah, Mark W; Kesting, Julie R; Sørensen, Dan;

    2011-01-01

    The extracts of five foliar fungal endophytes isolated from Pinus strobus (eastern white pine) that showed antifungal activity in disc diffusion assays were selected for further study. From these strains, the aliphatic polyketide compound 1 and three related sesquiterpenes 2-4 were isolated and c...... by spectroscopic analyses including 2D NMR, HRMS and by comparison to literature data where available. The isolated compounds 1, 2, and 5 were antifungal against both the rust Microbotryum violaceum and Saccharomyces cerevisae....

  17. Site index models for calabrian pine (Pinus brutia Ten.) in Thasos Island, Greece

    OpenAIRE

    Kyriaki Kitikidou; Diamantis Bountis; Elias Milios

    2011-01-01

    A site index model for Calabrian pine (Pinus brutia Ten.) in Thasos island (North Aegean sea, Greece) is presented. The model was fitted and validated from 150 stem analyses, obtained from 75 fixed-area plots from five experimental sites. Four height growth equations of difference form were tested and the BAILEY and CLUTTER (1974) function was considered appropriate due to its good performance with both fitting and validation data. The results show errors lower than 5% and little bias.

  18. Site index models for calabrian pine (Pinus brutia Ten. in Thasos Island, Greece

    Directory of Open Access Journals (Sweden)

    Kyriaki Kitikidou

    2011-01-01

    Full Text Available A site index model for Calabrian pine (Pinus brutia Ten. in Thasos island (North Aegean sea, Greece is presented. The model was fitted and validated from 150 stem analyses, obtained from 75 fixed-area plots from five experimental sites. Four height growth equations of difference form were tested and the BAILEY and CLUTTER (1974 function was considered appropriate due to its good performance with both fitting and validation data. The results show errors lower than 5% and little bias.

  19. Effect of tube shelters on the growth of young Turkish pines (Pinus brutia Ten., Pinaceae)

    OpenAIRE

    Leroy, Céline; Caraglio, Yves

    2003-01-01

    International audience; Young Turkish pine (Pinus brutia Ten.) individuals were planting, in the Montpellier region, with or without tube shelter alternating. The effect of these tubes was determinate thanks to the growth study of the main polycyclic stem (several elongation phases separated by rest periods). The analysis was done by a quantitative comparison of successive annual shoots and growth units on individuals of the same age, some with tube shelters and some without. The trees grown ...

  20. 樟子松育苗技术%Nursery Technology of Pinus sylvestnis

    Institute of Scientific and Technical Information of China (English)

    张晓青; 李冬梅

    2011-01-01

    The paper introduced Pinus sylvestris breeding techniques, including seed preparation, soil preparation, fertilizing, planting and cultivation and management, etc. , so as to provide technical reference in reproduction seedlings.%介绍了樟子松育苗技术,包括种子准备、整地施肥、播种以及抚育管理等内容,从而为樟子松的苗木繁殖提供技术参考。

  1. Climate-influenced ponderosa pine (Pinus ponderosa) seed masting trends in western Montana, USA

    OpenAIRE

    Christopher R. Keyes; Rubén Manso González

    2015-01-01

    Aim of study: The aim of this study was to analyze 10-year records of ponderosa pine (Pinus ponderosa) seed production, in order to confirm synchronic seed production and to evaluate cyclical masting trends, masting depletion effect, and climate-masting relationships. Area of study: The study area was located in a P. ponderosa stand in the northern Rocky Mountains (western Montana, USA). Material and methods: The study was conducted in one stand that had been subjected to a silvicul...

  2. Growth response of Pinus densiflora seedlings inoculated with three indigenous ectomycorrhizal fungi in combination

    OpenAIRE

    Dalong,M; Luhe,W; Guoting,Y; M Liqiang; Chun,L

    2011-01-01

    Pinus densiflora seedlings were inoculated with three indigenous ectomycorrhizal fungi (Cenococcum geophilum, Rhizopogon roseolus and Russula densifolia) in single-, two-, and three-species treatments. After 8 months, the colonization rates of each ectomycorrhizal species, seedling growth and the nutrition were assessed in each treatment. P. densiflora seedlings inoculated with different ECM species composition showed an increase in height and basal diameter and improved seedling root and sho...

  3. Labdane-type diterpenes active against acne from pine cones (Pinus densiflora).

    Science.gov (United States)

    Sultan, Md Zakir; Jeon, Young-Min; Moon, Surk-Sik

    2008-03-01

    Bioassay-guided extraction and fractionation of the aqueous methanolic extract of the cones of Pinus densiflora (Pinaceae) afforded one new labdane-type diterpene aldehyde, 15-nor-14-oxolabda-8(17),12 E-diene-18-oic acid, along with eight known diterpenes. Their structures were elucidated using spectroscopic methods as well as by comparison with previously reported data. The isolates showed antibacterial (Propionibacterium acnes) and antifungal activities.

  4. Factors affecting the emission of monoterpenes from red pine (Pinus densiflora)

    Energy Technology Data Exchange (ETDEWEB)

    Yokouchi, Y.; Ambe, Y.

    1984-08-01

    The mechanism of monoterpene emission from Pinus densiflora was studied using an environmentally controlled gas cabinet. It was found that monoterpene emission rate increases exponentially with temperature and is also influenced by light. These observations were explained reasonably by a mechanism whereby monoterpene emission rate depends on the monoterpene amount in the leaf oil and its saturated vapor pressure. 14 references, 2 figures, 1 table.

  5. Soil and Stocking Effects on Caliciopsis Canker of Pinus strobus L.

    OpenAIRE

    Isabel A. Munck; Thomas Luther; Stephen Wyka; Donald Keirstead; Kimberly McCracken; William Ostrofsky; Wayne Searles; Kyle Lombard; Jennifer Weimer; Bruce Allen

    2016-01-01

    Soil and stand density were found to be promising predictive variables associated with damage by the emerging disease of eastern white pine, Caliciopsis canker, in a 2014 survey with randomly selected eastern white pine (Pinus strobus L.) stands. The objective of this study was to further investigate the relationship between soil and stocking in eastern white pine forests of New England by stratifying sampling across soils and measuring stand density more systematically. A total of 62 eastern...

  6. Higher-than-present Medieval pine (Pinus sylvestris) treeline along the Swedish Scandes

    OpenAIRE

    2015-01-01

    The upper treeline of Scots pine (Pinus sylvestris L.) is renowned as a sensitive indicator of climate change and variability. By use of megafossil tree remains, preserved exposed on the ground surface, treeline shift over the past millennium was investigated at multiple sites along the Scandes in northern Sweden. Difference in thermal level between the present and the Medieval period, about AD 1000-1200, is a central, although controversial, aspect concerning the detection and attribution of...

  7. Dissipation of excess excitation energy of the needle leaves in Pinus trees during cold winters

    Science.gov (United States)

    Zhang, AO; Cui, Zhen-Hai; Yu, Jia-Lin; Hu, Zi-Ling; Ding, Rui; Ren, Da-Ming; Zhang, Li-Jun

    2016-12-01

    Photooxidative damage to the needle leaves of evergreen trees results from the absorption of excess excitation energy. Efficient dissipation of this energy is essential to prevent photodamage. In this study, we determined the fluorescence transients, absorption spectra, chlorophyll contents, chlorophyll a/ b ratios, and relative membrane permeabilities of needle leaves of Pinus koraiensis, Pinus tabulaeformis, and Pinus armandi in both cold winter and summer. We observed a dramatic decrease in the maximum fluorescence ( F m) and substantial absorption of light energy in winter leaves of all three species. The F m decline was not correlated with a decrease in light absorption or with changes in chlorophyll content and chlorophyll a/ b ratio. The results suggested that the winter leaves dissipated a large amount of excess energy as heat. Because the cold winter leaves had lost normal physiological function, the heat dissipation depended solely on changes in the photosystem II supercomplex rather than the xanthophyll cycle. These findings imply that more attention should be paid to heat dissipation via changes in the photosystem complex structure during the growing season.

  8. Effects Of Drought Stress on Germination in Fourteen Provenances of Pinus Brutia Ten. Seeds in Turkey

    Directory of Open Access Journals (Sweden)

    Hakan Şevik

    2015-02-01

    Full Text Available Pinus brutia Ten., Red pine, known to be tough drought resistant pine specie, could effectively be used for afforestation of disturbed areas. It is of great interest for the afforestation in arid zones. Appropriate seed sources for the specific areas guarantees reforestation success. Away from its native areas Pinus brutia Ten. is planted for its ornamental value and timber production purposes. Selection of drought resistant provenances can very well increase the survival success. In this study, the effects of water potential on germination were studied in fourteen provenances of Pinus brutia Ten. from Turkey. Water potentials between 0 and -8 bars were obtained using polyethylene glycol 6000 (PEG-6000 solutions. Seeds were kept for 35 day at 20 ± 0.5°C. A decrease in water potential produced a marked reduction in germination percentage and germination value. As a result, significant variations between the provenances were found. It was determined that, under a -8 bar water stress, Isparta-Bucak and Mersin-Silifke, respectively corresponding to 58% and 57% of the control group, were the least water stress affected provenances.

  9. Genome size and base composition of five Pinus species from the Balkan region.

    Science.gov (United States)

    Bogunic, F; Muratovic, E; Brown, S C; Siljak-Yakovlev, S

    2003-08-01

    The 2C DNA content and base composition of five Pinus (2 n=24) species and two Pinus subspecies from the Balkan region have been estimated by flow cytometry. P. heldreichii (five populations) and P. peuce (one population) were assessed for the first time, as also were subspecies of P. nigra (three populations-two of subspecies nigra and one of subspecies dalmatica) along with P. sylvestris, and P. mugo from the same region. The 2C DNA values of these Pinus ranged from 42.5 pg to 54.9 pg (41.7-53.8 x 10(9)bp), and the base composition was quite stable (about 39.5% GC). Significant differences were observed between two subspecies of P. nigra and even between two populations of subsp. nigra. The two other species (P. sylvestris and P. mugo) had 2C values of 42.5 pg and 42.8 pg, respectively, while that of P. peuce was 54.9 pg. These genome sizes are in accordance with published values except for P. sylvestris, which was 20% below estimates made by other authors.

  10. Antimicrobial activity of needle essential oil of Pinus peuce Griseb. (Pinaceae from Macedonian flora

    Directory of Open Access Journals (Sweden)

    Marija Karapandzova

    2012-03-01

    Full Text Available Chemical composition and antimicrobial activity of needle essential oil, obtained by hydrodistillation from wild Pinus peuce Griseb. (Pinaceae, growing on three different locations in R. Macedonia were investigated in period 2008/2009. Carried out GC/FID/MS analysis, one hundred and three constituents were identified belonging to the six different classes of components: monoterpene hydrocarbons, oxygenated monoterpenes, sesquiterpene hydrocarbons, oxygenated sesquiterpenes, diterpenes and other non-terpene components, representing 88.61/94.04% of the entire oil. The most abundant constituents were α-pinene (12.89/27.34%, β-pinene (6.16/13.13%, limonene + β-phellandrene (2.09/6.64% and bornyl acetate (2.92/11.67% as well as trans-(E-caryophyllene (4.63/7.13% and germacrene D (8.75/20.14%. Antimicrobial screening of Pinus peuce needle essential oil was made by hole-plate diffusion and broth dilution method against 13 bacterial isolates of Gram positive and Gram negative bacteria and one strain of Candida albicans. The most sensitive bacteria against tested Pinus peuce essential oils were Streptococcus pneumonia encompassing Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus agalactiae, Acinetobacter spp. and Streptococcus pyogenes. Minimal inhibitory concentrations (MICs of the oils ranged from 7.5- 62.5 µl/ml.

  11. Experimental study of Pinus densiflora Siebold et Zuccarini on Hyperlipidemia and lipid in Rats

    Directory of Open Access Journals (Sweden)

    Kim, Dae-Hyun

    2007-02-01

    Full Text Available In order to study the effects of Pinus densiflora on hyperlipidemia and lipid in rats, we divided the rats into groups(Normal group, Control group and Sample group and perfomed the experimental research. Hyperlipidemia and lipid in rats were induced by high fat diets for 8weeks. The sample group was administerd the extract of Pinus densiflora for 14 days and control group was administerd equal dose of oral. And then we measured the amount of serum triglyceride, Total cholesterol, LDL-cholesterol, HDL-cholesterol, Free Fatty Acid, phospholipid, Insuline, Laptin, Body weight, epididymis fat weight & rate, epididymis fat cell, Cardiac Risk Factor(CRF. The results were as followers : 1. Sample Group showed decreasing effects on Total cholesterol, Trigylceride, LDL-cholesterol, and Phospholipid levels in serum and CRF significantly. 2. Sample Group showed increasing effects on HDL-cholesterol level in serum significantly. 3. Sample Group showed decreasing effects on Insuline in serum significantly. 4. Sample Group showed increasing effects on Laptin in serum significantly. 5. Sample Group showed decreasing effects on Body weight, epididymis fat weight & rate, epididymis fat cell significantly. According to the above results, Pinus densiflora showed significant decreasing effects on hyperlipidemia and lipid in rats, it is considered that it is appropriate to apply for hyperlipidemia, obesity.

  12. Chemical composition changes in eucalyptus and pinus woods submitted to heat treatment.

    Science.gov (United States)

    Brito, J O; Silva, F G; Leão, M M; Almeida, G

    2008-12-01

    This study investigated the influence of heat treatment on the chemical composition of Eucalyptus saligna and Pinus caribaea var. hondurensis woods to understand its role in wood processing. E. saligna and P. caribaea var. hondurensis woods were treated in a laboratorial electric furnace at 120, 140, 160 and 180 degrees C to induce their heat treatment. The chemical composition of the resulting products and those from original wood were determined by gas chromatography. Eucalyptus and Pinus showed a significant reduction in arabinose, manose, galactose and xylose contents when submitted to increasing temperatures. No significant alteration in glucose content was observed. Lignin content, however, increased during the heat process. There was a significant reduction in extractive content for Eucalyptus. On the other hand, a slight increase in extractive content has been determined for the Pinus wood, and that only for the highest temperature. These different behaviors can be explained by differences in chemical constituents between softwoods and hardwoods. The results obtained in this study provide important information for future research and utilization of thermally modified wood.

  13. Laboratory Evaluation of the Anti-stain Efficacy of Crude Wood Vinegar for Pinus densiflora

    Directory of Open Access Journals (Sweden)

    Sabiha Salim

    2016-12-01

    Full Text Available In Korea, Pinus densiflora is one of the most important indigenous tree species in terms of making high-value wood products. Therefore, Korean sawmills exercise extreme caution to prevent fungal discoloration such as that caused by sapstains and molds on the timber. In this study, the effectiveness of using natural crude wood vinegar to inhibit sapstains and molds, especially on Pinus densiflora (Japanese red pine was examined. Pinus densiflora wood samples were dipped in absolute and diluted wood vinegar at different concentrations (1:1, 2:1, and 3:1; deionized water to wood vinegar dilution ratio for 3 minutes and immediately air-dried. In addition, volatile wood vinegar was also used in this study to imitate the condition of wood vinegar when exposed to open air. The degree of discoloration was examined and evaluated every 2, 4, 6, and 8 weeks according to the ASTM D4445-91 Standard Method for laboratory test. Crude wood vinegar inhibited sapstains more efficiently than it inhibited molds. Wood vinegar at a 1:1 concentration was found to be the most optimum treatment for inhibiting sapstains for at least 8 weeks.

  14. PRODUÇÃO DE MUDAS DE Pinus elliottii Engelm. MICORRIZADAS EM SOLO ARENOSO

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    Robson Andreazza

    2003-05-01

    Full Text Available A eficiência de diferentes isolados de fungos ectomicorrízicos foi avaliada para produção de mudas de pinus em solo arenoso. Os tratamentos foram: testemunha; fungo Rh 117; fungo Pt Siv.1; fungo FSE – RS (nativo e fungo F1 – RS (nativo. O trabalho foi desenvolvido em casa de vegetação cujas mudas foram produzidas em solo sob processo de “arenização”, coletado no município de São Francisco de Assis, RS. Determinoaram-se a massa verde da parte aérea e radicular, massa seca da parte aérea, altura de planta, comprimento e área superficial específica radicular, colonização micorrízica e teores de nitrogênio, fósforo e potássio. Os fungos F1 – RS e Pt Silv.1 beneficiaram as mudas de pinus nos parâmetros radiculares como massa verde radicular, comprimento e área superficial específica da raiz. A associação micorrízica não favoreceu a absorção de nutrientes (N, P e K e no desenvolvimento da parte área da muda de pinus.

  15. Produção de mudas de Pinus elliottii Engelm. micorrizadas em solo arenoso.

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    Rodrigo Ferreira da Silva

    2010-08-01

    Full Text Available A eficiência de diferentes isolados de fungos ectomicorrízicos foi avaliada para produção de mudas de pinus em solo arenoso. Os tratamentos foram: testemunha; fungo Rh 117; fungo Pt Siv.1; fungo FSE - RS (nativo e fungo F1 - RS (nativo. O trabalho foi desenvolvido em casa de vegetação cujas mudas foram produzidas em solo sob processo de "arenização", coletado no município de São Francisco de Assis, RS. Determinoaram-se a massa verde da parte aérea e radicular, massa seca da parte aérea, altura de planta, comprimento e área superficial específica radicular, colonização micorrízica e teores de nitrogênio, fósforo e potássio. Os fungos F1 - RS e Pt Silv.1 beneficiaram as mudas de pinus nos parâmetros radiculares como massa verde radicular, comprimento e área superficial específica da raiz. A associação micorrízica não favoreceu a absorção de nutrientes (N, P e K e no desenvolvimento da parte área da muda de pinus.

  16. Distribution of chemical compartments of soil organic matter and c stocks of a cambisol from south Brazil as affected by Pinus afforestation

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    Henrique Cesar Almeida

    2012-01-01

    Full Text Available Distribution and stocks of soil organic matter (SOM compartments after Pinus monoculture introduction in a native pasture area of a Cambisol, Santa Catarina, Brazil, were investigated. Pinus introduction increased soil acidity, content of exchangeable Al+3 and diminished soil nutrients. Nevertheless, soil C stock increased in all humic fractions of the 0-5 cm layer after Pinus afforestation. In the subsurface, the vegetation change only promoted SOM redistribution from the NaOH-extractable humic substances to a less hydrophobic humin fraction. Under Pinus, soil organo-mineral interactions were relevant up to a 15 cm depth, while in pasture environment, this mechanism occurred mainly in the surface layer.

  17. Adaptation of stone pines Pinus sibirica Du Tour and Pinus koraiensis Siebold et Zucc. to various environmental factors in the testing sites

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    G. V. Kuznetsova

    2016-10-01

    Full Text Available Provenance trials of Pinus sibirica Du Tour and Pinus koraiensis Siebold et Zucc. (here after «studied species» were studied. In our study we assessed the growth parameters as well as anatomical and morphological parameters of the studied species corresponding to different provenances of their testing in the south of the Krasnoyarsk and Khabarovsk territories.We determined that the growth rate of trees corresponding to different provenances is determined not only by the inherited characteristics, but also by adaptation. At both experimental regions the offspring of trees corresponding to local provenance are clearly better adapted. Nevertheless, at Krasnoyarsk Krai provenance trials, we found that the phenotypic indicators and degree of preservation of the offspring of two Korean pine corresponding to Obluchensky and Chuguevsky provenances are at the same level as for the local Siberian pine. Tree rings widths have been measured for the Siberian pine corresponding to different provenances at both plantations. We conclude that at the Ermakovskoe plantation there is a positive impact of the environmental conditions on tree-ring width for Korean pine corresponding to different provenances, and in Khabarovsk Krai there is a negative impact of the environmental conditions on tree-ring width for the Siberian pine corresponding to different provenances.

  18. Composition and Bioactivity of Lipophilic Metabolites from Needles and Twigs of Korean and Siberian Pines (Pinus koraiensis Siebold & Zucc. and Pinus sibirica Du Tour).

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    Shpatov, Alexander V; Popov, Sergey A; Salnikova, Olga I; Kukina, Tatyana P; Shmidt, Emma N; Um, Byung Hun

    2017-02-01

    Lipophilic extractive metabolites in different parts of the shoot system (needles and defoliated twigs) of Korean pine, Pinus koraiensis, and Siberian pine, Pinus sibirica, were studied by GC/MS. Korean pine needles comprised mainly bornyl p-coumarate, heterocyclic 15-O-functionalized labdane type acids (lambertianic acid), 10-nonacosanol, sterols and their esters. While Siberian pine needles contained less bornyl p-coumarate, lambertianic acid, sterols and their esters, but were richer in other 15-O-functionalized labdane type acids. The major components of the twig extract of P. koraiensis were lambertianic acid, abietane and isopimarane type acids, cembrane type alcohols, 8-O-functionalized labdanoids, sterols, sterol esters, and acylglycerols. The same extract of P. sibirica differed in larger amounts of other 15-O-functionalized labdane type acids and pinolenic acid glycerides, but in less quantities of cembranoids and 8-O-functionalized labdanoids. The labdane type pinusolic acid was detected for the first time in Korean pine. P. koraiensis was found to be unique in the genus for an ability to synthesize phyllocladane diterpenoids. The content of bound Δ(5) -unsaturated polymethylene-interrupted fatty acids in the twig extracts of the both pines was similar or superior to that in their seed oil. Among the pines' metabolites tested isocembrol was strongest in inhibition of both α-glucosidase (IC50 2.9 μg/ml) and NO production in activated macrophages (IC50 3.6 μg/ml).

  19. Spatial Genetic Structure within and among Seed Stands of Pinus engelmannii Carr. and Pinus leiophylla Schiede ex Schltdl. & Cham, in Durango, Mexico

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    María Elena Ortiz-Olivas

    2017-01-01

    Full Text Available Studies of spatial genetic structure (SGS are important because they offer detailed insights into historical demographic and evolutionary processes and provide important information regarding species conservation and management. Pinus engelmannii and P. leiophylla var. leiophylla are two important timber tree species in Mexico, covering about 2.5 and 1.9 million hectares, respectively. However, studies in relation to population genetics are unfortunately scant. The aim of this research was to use amplified fragment length polymorphisms (AFLP analysis to identify potential differences in spatial genetic structure within and among seven Pinus engelmannii and nine P. leiophylla var. leiophylla seed stands in Durango, Mexico. Within the 16 seed stands of the two tested pine species, no significant SGS was detected, although SGS was detected among the seed stands. We concluded that the collection of seed in only some seed stands should not significantly alter the degree of genetic differentiation within the (collected seed. Distances between seed orchards and pollen propagators of more than 24 km for P. engelmannii and 7 km for P. leiophylla may be sufficient to limit contamination. Finally, local seeds should be used for (reforestation.

  20. Larvicidal effect of dried leaf extracts from Pinus caribaea against Aedes aegypti (Linnaeus, 1762 (Diptera: Culicidae Efeito larvicida dos extratos de folhas secas de Pinus caribaea contra Aedes aegypti (Linnaeus, 1762 (Diptera: Culicidae

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    Luiz Alberto Kanis

    2009-08-01

    Full Text Available In this study, the larvicidal activity of dried leaf extracts from Pinus caribaea Morelet against Aedes aegypti was evaluated for the first time. Pinus caribaea extracts were obtained by macerating dried leaves in alkaline hydroethanol, ethanol and acetone solutions followed by evaporation under reduced pressure. The lignin content was quantified using the thioglycolic acid complexation method. Lethality bioassays (LC50 and LC90 were carried out in accordance with the recommendations of the World Health Organization. The results showed that the acetone extract from Pinus caribaea was more active, and that larvicidal activity was associated with lignin concentration.Neste trabalho foi avaliada pela primeira vez a atividade larvicida contra Aedes aegypti de extratos obtido a partir de folhas secas de Pinus caribae. Os extratos de Pinus caribaea foram obtidos a partir da maceração de folhas secas em solução hidroetanólica alcalina, etanol e acetona, seguido de evaporação sob pressão reduzida. O teor de lignina foi quantificado usando o método de complexação com ácido tioglicóliclo. Os ensaios de letalidade foram conduzidos de acordo com a recomendação da Organização Mundial da Saúde. Os resultados demonstraram que o extrato obtido com acetona foi mais ativo, e a atividade larvicida esta associada com a concentração de ligninas.

  1. Biometrical study of some individuals chosen from Pinus mugo turra populations in the peat bog "Bór na Czerwonem"

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    Alina Bączkiewicz

    2014-01-01

    Full Text Available Sixteen individuals were sampled for study of variation in 17 anatomical and morphological characters. Only individuals of low polycormic growth (trait typical for Pinus mugo Turra connected with incurved one-year-cone stipes (a similar situation exists in Pinus sylvestris L. were chosen, thus the sample studied cannot be treated as a random one. It has been shown by multivariate statistical analysis that these 16 individuals are quite different from each other, Mahalanobis'generalized distances between them being nearly 50% significantly different from 0. The sample studied in this respect is distinctly different from pure stands of both putative parental species (i.e. Pinus mugo and P. sylvestris. Every plant studied shows a different combination of traits typical (or nearly typical for both the above-mentioned species and traits that are truly intermediate between them. The results support the frequently expressed opinion that the mountain pine population from the peat bog "Bór na Czerwonem" is, in fact, a hybrid swarm formed by hybridization between Pinus mugo and Pinus sylvestris.

  2. ATIVIDADE ANTIMICROBIANA IN VITRO DO LÁTEX DO AVELOZ (Euphorbia tirucalli L., PINHÃO BRAVO (Jatropha mollissima L. E PINHÃO ROXO (Jatropha gossypiifolia L. SOBRE MICRORGANISMOS PATOGÊNICOS.

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    Francisco Angelo Gurgel da Rocha

    2010-01-01

    Full Text Available Por milênios, o Homem tem explorado a flora como recurso terapêutico. Muitas das espécies medicinais utilizadas possuem fitoquímicos com potencial aplicação no controle do crescimento microbiano. A resistência microbiana crescente representa um sério risco à Saúde Coletiva, sendo necessário o desenvolvimento de projetos que visem a prospecção e/ou o desenvolvimento de novas drogas antimicrobianas. Na Caatinga da Região do Seridó do RN são encontradas as plantas medicinais Jatropha mollissima (Pohl Baill (Pinhão-bravo, a Jatropha gossypiifolia L (Pinhão-roxo e Euphorbia tirucalli L (Aveloz, pertencentes à Família Euphorbiaceae, na qual estão presentes fitoquímicos antimicrobianos. O presente projeto objetivou caracterizar a ação antibacteriana do látex destas espécies vegetais contra os patógenos Staphylococcus aureus, Salmonella typhimurium, Salmonella typhi e Listeria monocytogenes. Duplicatas de Placas de Petri contendo ágar Mueller-Hinton foram semeadas em superfície com culturas padronizadas de cada microrganismo. Foram perfurados poços de 10 mm de diâmetro nos quais se inoculou 100 µL das diluições dos látex testados. As placas foram incubadas a 35±2oC/24 h. Os halos de inibição quando existentes foram medidos e registrados. L. monocytogenes apresentou a maior sensibilidade ao látex de J. mollissima; S. aureus foi inibido por J. mollissima e J. gossypiifolia, sendo mais sensível no primeiro caso. S. typhi e S. typhimurium apresentaram os maiores halos de inibição (J. gossypiifolia, sendo S. typhi mais sensível ao látex de J. gossypiifolia e S. typhimurium ao de J. mollissima. E. tirucalli não apresentou atividade inibitória. Padrões de sazonalidade parecem influenciar a capacidade inibitória do látex. Recomendamos que sejam efetuados estudos mais detalhados acerca das propriedades antimicrobianas do látex de J. mollissima e J. gossypiifolia. PALAVRAS-CHAVE: atividade antimicrobiana, Jatropha

  3. Eficiência dos herbicidas oxadiazil, oxadiazon, oxyfluorfen e Imazapyr sobre a cultura de pinus Efficiency of the oxadiazil, oxadiazon, oxyfluorfen and imazapyr herbicides on Pinus cropping

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    Wilson da Silva

    1999-08-01

    Full Text Available Avaliou-se a seletividade de alguns herbicidas para mudas de Pinus caribaea var. Hondurensis e suas eficiências no controle de plantas daninhas. As mudas com 20 cm de altura, produzidas em tubetes, foram transplantadas num espaçamento de 2,0 m x 2,0 m, em parcelas de quatro fileiras com 10,0 m de comprimento. Os tratamentos utilizados foram oxadiazil (600; 800 e 1000 g ha-1, oxadiazon (1140; 1520 e 1900 g ha-1, oxyfluorfen (720 g ha-1, imazapyr (250 g ha-1 e testemunhas (capinada e sem capina, dispostos em blocos ao acaso, com quatro repetições. Os herbicidas foram aplicados sete dias após o transplante, usando-se pulverizador costal, pressurizado com CO2, calibrado para 200 L ha- 1 de calda. As principais plantas daninhas com maior infestação foram: Brachiaria plantaginea e Ipomoea grandifolia e com menor infestação: Galinsoga parviflora e Bidens pilosa. O oxadiazil apresentou excelente controle de B. plantaginea, I. grandifolia e G. parviflora, não sendo eficiente para controle de B. pilosa, embora proporcionasse controle superior ao observado pelo oxadiazon. O oxadiazon foi eficiente até 45 dias após o tratamento para B. plantaginea, G. parviflora e até 90 dias para I. grandifolia. Tanto o oxyfluorfen quanto o imazapyr apresentaram excelente controle das plantas daninhas. De todos os herbicidas avaliados, apenas o imazapyr não deve ser recomendado para aplicação sobre o dossel de Pinus caribaea var. Hondurensis, em face da toxicidade provocada.The selectivity of some herbicides were evaluated on Pinus caribaea var. Hondurensis seedlings as well as these herbicide efficiencies in the weed control. When the tubule-produced seedlings were 20 cm height they were transplanted at 2,0 m x 2,0 m spacings in fourrows plots with 10,0 m length. The treatments with oxadiazil (600; 800 and 1000 g ha-1, oxadiazon (1140; 1520 and 1900 g ha-1, oxyfluorfen (720 g ha-1, imazpyr (250 g ha-1 and controls (weeded and without weeding were disposed

  4. Chemotaxonomic implications of the n-alkane composition and the nonacosan-10-ol content in Picea omorika, Pinus heldreichii, and Pinus peuce.

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    Nikolić, Biljana; Tešević, Vele; Bojović, Srdjan; Marin, Petar D

    2013-04-01

    The n-alkane composition and the nonacosan-10-ol content in the needle cuticular waxes of Serbian spruce (Picea omorika), Bosnian pine (Pinus heldreichii), and Macedonian pine (Pinus peuce) were compared. The amount of nonacosan-10-ol in the needle waxes of P. omorika was higher than those in P. heldreichii and P. peuce. The range of n-alkanes was also wider in P. omorika (C18 -C35 ) than in P. heldreichii and P. peuce (C18 -C33 ). The dominant n-alkanes were C29 in the needle waxes of P. omorika, C23 , C27 , and C25 in those of P. heldreichii, and C29 , C25 , C27 , and C23 in those of P. peuce. The waxes of P. omorika contained higher amounts of n-alkanes C29 , C31 , and C33 , while those of P. heldreichii and P. peuce had higher contents of n-alkanes C21 , C22 , C23 , C24 , and C26 . The principal component analysis of the contents of nine n-alkanes showed a clear separation of the Serbian spruce populations from those of the two investigated pine species, which partially overlapped. The separation of the species was due to high contents of the n-alkanes C29 and C31 (P. omorika), C19 , C20 , C21 , C22 , C23 , and C24 (P. heldreichii), and C28 (P. peuce). Cluster analysis also showed a clear separation between the P. omorika populations on one side and the P. heldreichii and P. peuce populations on the other side. The n-alkane and terpene compositions are discussed in the light of their usefulness in chemotaxonomy as well as with regard to the biogeography and phylogeny of these rare and endemic conifers.

  5. Response of needle dark respiration of Pinus koraiensis and Pinus sylvestriformis to elevated CO2 concentrations for four growing seasons' exposure

    Institute of Scientific and Technical Information of China (English)

    ZHOU YuMei; HAN ShiJie; ZHANG HaiSen; XIN LiHua; ZHENG JunQiang

    2007-01-01

    The long-term effect of elevated CO2 concentrations on needle dark respiration of two coniferous species-Pinus koraiensis and Pinus sylvestriformis on the Changbai Mountain was investigated using open-top chambers. P. Koraiensis and P. Sylvestriformis were exposed to 700,500μmol·mol-1 CO2 and ambient CO2(approx.350 μmol·mol-1)for four growing seasons. Needle dark respiration was measurd during the second, third and fourth growing seasons' exposure to elevated CO2.The results showed that needle dark respiration rate increased for P. Koraiensis and P. Sylvestriformis grown at elevated CO2 concentrations during the second growing season, could be attributed to the change of carbohydrate and/or nitrogen content of needles. Needle dark respiration of P. Koraiensis was stimulated and that of P. Sylvestriformis was inhibited by elevated CO2 concentrations during the third growing season. Different response of the two tree species to elevated CO2 mainly resulted from the difference in the growth rate. Elevated CO2 concentrations inhibited needle dark respiration of both P. Koraiensis and P. Sylvestriformis during the fourth growing season. There was consistent trend between the short-term effect and the long-term effect of elevated CO2 on needle dark respiration in P. Sylvestriformis during the third growing season by changing measurement CO2 concentrations. However, the short-term effect was different from the long-term effect for P. Koraiensis. Response of dark respiration of P. Koraiensis and P. Sylvestriformis to elevated CO2 concentrations was related to the treatment time of CO2 and the stage of growth and development of plant. The change of dark respiration for the two tree species was determined by the direct effect of CO2 and long-term acclimation. The prediction of the long-term response of needle dark respiration to elevated CO2 concentration based on the short-term response is in dispute.

  6. Cambial activity and xylem cell development in Pinus cembra and Pinus sylvestris at their climatic limits in the Eastern Alps in 2007.

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    Swidrak, Irene; Gruber, Andreas; Oberhuber, Walter

    2011-12-20

    It has been frequently stressed that at distributional boundaries, like at the Alpine timberline and within dry inner Alpine environments, tree growth will be affected first by changing climate conditions. Climate in 2007 was characterized by the occurrence of exceptionally mild temperatures in spring (3.4 and 2.7 °C above long-term mean (LTM) at timberline and the valley sites, respectively) with an almost continuous drought period recorded in April and slightly warmer than average temperatures throughout summer (1.3 °C above LTM at both sites). We compared temporal dynamics of cambial activity and xylem cell development in Pinus cembra at the Alpine timberline (1950 m a.s.l.) and Pinus sylvestris at a xeric inner Alpine site (750 m a.s.l.) by repeated cellular analyses of micro-cores (n = 5 trees/site). While onset of wood formation in P. sylvestris and P. cembra differed by about two weeks (12 and 27 April, respectively), maximum daily growth rates peaked on 6 May at the valley site and on 23 June at timberline. At both sites maximum tracheid production was reached prior to occurrence of more favourable climatic conditions during summer, i.e. an increase in precipitation and temperature. Xylem formation ended on 31 August and 28 October at the xeric site and at timberline, respectively. This study demonstrates the plasticity of tree-ring formation along an altitudinal transect in response to water availability and temperature. Whether early achievement of maximum growth rates is an adaptation to cope with extreme environmental conditions prevailing at limits of tree growth needs to be analysed more closely by taking belowground carbon allocation into account.

  7. Isoenzymatic variability in progeny of Pinus mugo Turra x Pinus sylvestris L. hybrids from Bór na Czerwonem, in experimental culture

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    Maria A. Bobowicz

    2014-01-01

    Full Text Available The article describes the genetic structure of 12-year old seedlings. They were grown in an experimental culture representing the progeny of natural hybrids of Pinus mugo Turra and Pinus sylvestris L. from the peatbog Bór na Czerwonem. The studies were carried out on seedlings, employing electrophoresis of enzymatic proteins from winter buds. Parallel isoenzymatic analyses were also conducted for trees of P. mugo Turra from Tatra Mountains and for P. sylvestris L. trees from Białowieża in order to determine similarities of the hybrids to the 'pure' species, thought to represent parental forms, and for natural hybrids from Bór na Czerwonem. The obtained electrophoretic patterns made the determination of genetic structure of the population possible, namely of the observed heterozygosity, expected heterozygosity, Wright's fixation index and coefficients of similarity according to Nei and Hedrick. Genetic distances between the populations were also estimated. The variability of 11 enzymatic loci demonstrated the highest level of variability in the population of hybrids. Coefficients of genetic differentiation (GST and of gene flow, (Nm suggested a close relationship of the hybrids to P. mugo which may indicate an introgressive type of the hybrid swarm, from which the studied trees originated. The analysis of genetic similarity coefficients, according to Nei and Hedrick, demonstrated also an extensive similarity of the two hybrid groups to P. mugo. However, upon analysis of individual enzymatic loci, some of them highly resembled P. mugo while others were similar to P. sylvestris. The closest genetic similarity was demonstrated for the group of hybrids from the peatbog and the respective of hybrides group from culture. The variable gene expression as well as asymmetric inheritance of isoenzymes provided additional evidence of the introgressive character of the studied trees from the hybrid population.

  8. The Influence of Pinus brutia on the Water Balance of Fractured Mediterranean Mountain Environments

    Science.gov (United States)

    Eliades, Marinos; Bruggeman, Adriana; Lubczynski, Maciek; Christou, Andreas

    2016-04-01

    In dry Mediterranean environments, both rainfall and temperature vary throughout the year and frequent droughts occur. The mountainous topography is characterized by steep slopes, often leading to shallow soil layers with limited water storage capacity. While for most of the tree species, these conditions can be characterized as unfavourable, Pinus brutia trees manage to survive and thrive. The main objective of this study is to define and quantify the water balance components of a Pinus brutia forest at tree level. Our study was conducted from 30/12/2014 until 31/09/2015 in an 8966-m2 fenced area of Pinus brutia forest. The site is located on the northern foothills of Troodos mountain at 620 m elevation, in Cyprus. The slope of the site ranged between 0 and 82%. The average daily minimum temperature is 5 0C in January and the average daily maximum temperature is 35 oC in August. The mean annual rainfall is 425 mm. We measured the diameter at breast height (DBH) from a total of 122 trees. Based on the average DBH, four trees were selected for monitoring (two were above the average DBH and two were below). We measured soil depth in a 1-m grid around each of the four selected trees. We processed soil depths in ArcGIS software (ESRI) to create a soil depth map. We used a Total Station and a differential GPS for the creation of a high resolution DEM of the area covering the four selected trees. We installed soil moisture sensors at 15-cm depth at distances of 1 and 2 m from the selected trees and a second sensor at 30-cm depth when the soil was deeper than 20 cm.. We randomly installed four metric manual rain gauges under each trees' canopy to measure throughfall and for stemflow we installed a plastic tube around each tree trunk and connected it to a manual rain gauge. We used six sap flow heat ratio method instruments to determine sap flow rates of the Pinus brutia trees. Two trees had one sensor installed at 1.3 m height facing north. The remaining trees had two sap

  9. Reproductive biology and early establishment of Pinus elliottii var. elliottii in Brazilian sandy coastal plain vegetation: implications for biological invasion

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    Fernando Campanhã Bechara

    2013-04-01

    Full Text Available Pinus is the most invasive woody taxon, exceeded only by herbaceous plants. This study reports the reproductive biology and early establishment of Pinus elliottii Engelm. var. elliottii, describing its invasive properties in a protected natural area of the Brazilian coastal sandy plains. We evaluated the seed germination and rain, longevity of seed viability and the initial dynamics of the seedlings of Pinus elliottii var elliottii through field and laboratory experiments. We recorded a continuous seed rain of about 204.0 viable seeds m- 2 per year, with a 90 % germination rate. The seeds exhibited a low longevity of viability in the soil and a dense, permanent seedling bank that may explain the high levels of pine invasion. The environmental impact caused by the pine's biological invasion suggests the recommendation for its immediate eradication, together with a restoration plan to restitute the native biodiversity gradually.

  10. Inhibitory effects of phenolic compounds from needles of Pinus densiflora on nitric oxide and PGE2 production.

    Science.gov (United States)

    Kwon, Joo Hee; Kim, Jee Hun; Choi, Sun Eun; Park, Kwan Hee; Lee, Min Won

    2010-12-01

    The needles of Pinus densiflora Siebold et Zuccarini, a representative Pinus species that grows in Korea, have been used in oriental traditional medicine as remedies for rheumatitis, hemorrhage, cancer, etc. Phytochemical examination of the needles of Pinus densiflora Siebold et Zuccarini led to the isolation of four lignans, one flavan-3-ol, two flavonols and one organic acid. They were identified as icariside E(4) (1), cupressoside A (2), schizandriside (3), (+)-isolariciresinol (4), (+)-catechin (5), quercetin 3-O-β-D-glucopyranoside (6), 5,7,8,4'-tetrahydroxy-3-methoxy-6-methylflavone 8-O-β-D-glucopyranoside (7) and (-)-shikimic acid (8). In order to evaluate the anti-inflammatory effects of these compounds, their inhibitory activities against nitric oxide and prostaglandin E(2) production in IFN-γ- and lipopolysaccharide-stimulated RAW 264.7 cells were examined.

  11. Chemical, Antioxidant and Antimicrobial Investigations of Pinus cembra L. Bark and Needles

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    Anca Miron

    2011-09-01

    Full Text Available The chemical constituents and biological activity of Pinus cembra L. (Pinaceae, native to the Central European Alps and the Carpathian Mountains, are not well known. The aim of the present work was to examine the phenolic content, antioxidant and antimicrobial effects of hydromethanolic extracts of Pinus cembra L. bark and needles. Bark extract had higher concentrations of total phenolics (299.3 vs. 78.22 mg gallic acid equivalents/g extract, flavonoids (125.3 vs. 19.84 mg catechin equivalents/g extract and proanthocyanidins (74.3 vs. 12.7 mg cyanidin equivalents/g extract than needle extract and was more active as a free radical scavenger, reducing agent and antimicrobial agent. The EC50 values in the 2,2-diphenyl-1-picrylhydrazyl (DPPH, 2,2'-azino-bis(3-ethylbenzo-thiazoline-6-sulfonic acid diammonium salt (ABTS and reducing power assays were 71.1, 6.3 and 26 mg/mL for bark extract and 186.1, 24 and 104 mg/mL for needle extract, respectively. In addition, needle extract showed ferrous ions chelating effects (EC50 = 1,755 μg/mL. The antimicrobial effects against Staphylococcus aureus, Sarcina lutea, Bacillus cereus, Escherichia coli, Pseudomonas aeruginosa and Candida albicans were assessed by the agar diffusion method. Both extracts (4 mg/well were active against all the microorganisms tested; bark extract showed higher inhibition on all strains. These results indicate that Pinus cembra L. bark and needles are good sources of phytochemicals with antioxidant and antimicrobial activities.

  12. Chemical, antioxidant and antimicrobial investigations of Pinus cembra L. bark and needles.

    Science.gov (United States)

    Apetrei, Cristina Lungu; Tuchilus, Cristina; Aprotosoaie, Ana Clara; Oprea, Adrian; Malterud, Karl Egil; Miron, Anca

    2011-09-13

    The chemical constituents and biological activity of Pinus cembra L. (Pinaceae), native to the Central European Alps and the Carpathian Mountains, are not well known. The aim of the present work was to examine the phenolic content, antioxidant and antimicrobial effects of hydromethanolic extracts of Pinus cembra L. bark and needles. Bark extract had higher concentrations of total phenolics (299.3 vs. 78.22 mg gallic acid equivalents/g extract), flavonoids (125.3 vs. 19.84 mg catechin equivalents/g extract) and proanthocyanidins (74.3 vs. 12.7 mg cyanidin equivalents/g extract) than needle extract and was more active as a free radical scavenger, reducing agent and antimicrobial agent. The EC₅₀ values in the 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2'-azino-bis(3-ethylbenzo-thiazoline-6-sulfonic acid) diammonium salt (ABTS) and reducing power assays were 71.1, 6.3 and 26 mg/mL for bark extract and 186.1, 24 and 104 mg/mL for needle extract, respectively. In addition, needle extract showed ferrous ions chelating effects (EC₅₀ = 1,755 μg/mL). The antimicrobial effects against Staphylococcus aureus, Sarcina lutea, Bacillus cereus, Escherichia coli, Pseudomonas aeruginosa and Candida albicans were assessed by the agar diffusion method. Both extracts (4 mg/well) were active against all the microorganisms tested; bark extract showed higher inhibition on all strains. These results indicate that Pinus cembra L. bark and needles are good sources of phytochemicals with antioxidant and antimicrobial activities.

  13. Metal(loid) allocation and nutrient retranslocation in Pinus halepensis trees growing on semiarid mine tailings.

    Science.gov (United States)

    Parraga-Aguado, Isabel; Querejeta, Jose-Ignacio; González-Alcaraz, María Nazaret; Conesa, Hector M

    2014-07-01

    The goal of this study was to evaluate internal metal(loid) cycling and the risk of metal(loid) accumulation in litter from Pinus halepensis trees growing at a mine tailing disposal site in semiarid Southeast Spain. Internal nutrient retranslocation was also evaluated in order to gain insight into the ability of pine trees to cope with the low-fertility soil conditions at the tailings. We measured metal(loid) concentrations in the foliage (young and old needles), woody stems and fresh leaf litter of pine trees growing on tailings. The nutrient status and stable isotope composition of pine foliage (δ(13)C, δ(15)N, δ(18)O as indicators of photosynthesis and water use efficiency) were also analyzed. Tailing soil properties in vegetation patches and in adjacent bare soil patches were characterized as well. Significant amounts of metal(loid)s such us Cd, Cu, Pb and Sb were immobilized in the woody stems of Pinus halepensis trees growing on tailings. Leaf litterfall showed high concentrations of As, Cd, Sb, Pb and Zn, which thereby return to the soil. However, water extractable metal(loid) concentrations in tailing soils were similar between vegetation patches (mineral soil under the litter layer) and bare soil patches. The pines growing on mine tailings showed very low foliar P concentrations in all leaf age classes, which suggests severe P deficiency. Young (current year) needles showed lower accumulation of metal(loid)s, higher nutrient concentrations (P and K), and higher water use efficiency (as indicated by and δ(13)C and δ(18)O data) than older needles. Substantial nutrient resorption occurred before leaf litterfall, with 46% retranslocation efficiency for P and 89% for K. In conclusion, phytostabilization of semiarid mine tailings with Pinus halepensis is feasible but would require careful monitoring of the trace elements released from litterfall, in order to assess the long term risk of metal(loid) transfer to the food chain.

  14. Effect of elevated ambient CO2 concentration on water use efficiency of Pinus sylvestriformis

    Institute of Scientific and Technical Information of China (English)

    Han Shijie; Zhang Junhui; Wang Chenrui; Zou Chunjing; Zhou Yumei; Wang Xiaochun

    1999-01-01

    Pinus sylvestriformis is an important species as an indicator of global climate changes in Changbai Mountain, China. The water use efficiency (WUE) of this species ( 11-year old ) was studied on response to elevated CO2 concentration at 500± 100 μL· L-1 by directly injecting CO2 into the canopy under natural condition in 1998-1999. The results showed that the elevated CO2 concentration reduced averagely stomatal opening, stomatal conductance and stomatal density to 78%, 80% and 87% respectively, as compared to normal ambient. The elevated CO2 reduced the transpiration and enhances the water use efficiency (WUE) of plant.

  15. Fire Burning Characteristics, Attacking Techniques and Safety Precautions in Pinus pumila Scrubs

    Institute of Scientific and Technical Information of China (English)

    2010-01-01

    In China, Pinus pumila is mainly distributed in Daxing’anling, while those growing in the eastern Daxing’anling are primarily covered under the jurisdiction of Huzhong Forest Bureau. P. pumila is an evergreen shrub, 3-6 m high, and always grows vigorously. Its trunks creep along the ground, usually 10 m long or more. It is easily flammable because of the large amounts of resins and volatile oils in its body. Attacking forest fires is a dangerous work, especially in P. pumila scrubs. The fires in P. pumila s...

  16. Pinus chiapensis: un enfoque ecológico de su anatomía foliar

    OpenAIRE

    E. Vilela; Salvador Acosta Castellanos

    2000-01-01

    Se registraron datos ecológicos y caracteres anatómicos de acículas de Pinus chiapensis, una especie amenazada, en el estado de Oaxaca, México. Evidencias fitogeográficas sugieren que la distribución actual de esta especie es relictual. Aunque habita áreas de alta humedad y precipitación, las hojas aciculares son xeromórficas (presencia de estomas hundidos, cutícula gruesa y esclerénquima subepidérmico). Estos resultados apoyan la hipótesis de una adaptación paleoclimática de la especie....

  17. Response of seedlings growth of Pinus sylvestriformis to atmospheric CO2 enrichment in Changbai Mountain

    Institute of Scientific and Technical Information of China (English)

    Han S hijie; Wang Chen rui; Zhang Junhui; Zou Chunjing; Zhou Yumei; Wang Xiaochun

    1999-01-01

    The biomass and ratio of root-shoot of Pinus sylvestriformis seedlings at CO2 concentration of 700 μL· L-1 and 500 μL· L-1 were measured using open-top chambers (OTCs) in Changbai Mountain during Jun.to Oct. in 1999. The results showed that doubling CO2 concentration was benefit to seedling growth of the species (500 μL· L-1 was better than 700 μL-L-1 ) and the biomass production was increased in both aboveground and underground parts of seedlings. Carbon transformation to roots was evident as rising of CO2 concentration.

  18. Consumption of seeds of southwestern white pine (Pinus strobiformis) by Black Bear (Ursus americanus)

    Science.gov (United States)

    Mattson, David J.; Arundel, Terry A.

    2013-01-01

    We report a discovery of black bears (Ursus americanus) consuming seeds of southwestern white pine (Pinus strobiformis) on north slopes of the San Francisco Peaks near Flagstaff, Arizona, in high-elevation, mixed-species conifer forest. In one instance, a bear had obtained seeds from cones excavated from a larder horde made by a red squirrel (Tamiasciurus hudsonicus). Consumption of seeds of southwestern white pine by bears had not been previously documented. This discovery adds to the number of species of pine used by bears for food as well as the geographic range within which the behavior occurs.

  19. Storage products and tissue interaction in the ovule of Pinus silvestris (L.

    Directory of Open Access Journals (Sweden)

    F. M. Engels

    2014-01-01

    Full Text Available The organel-sequence in ovular cells of Pinus silvestris was investigated by light- and electronmicroscopy during the post-pollination and pre-fertilization period. Changes in starch and lipid storage suppose starch to be a pool for lipid synthesis and a reserve for ovule development. The base nucellus plays an important role in the distribution of metabolites all over the ovular tissues. Lipid, starch and callose are of interest for the cells to protect them against low temperatures by means of isolation, antifreeze and plug formation respectively.

  20. Genetic divergence of Scots pine (Pinus sylvestris L. populations in Serbia revealed by RAPD

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    Lučić A.

    2011-01-01

    Full Text Available The ability of random amplified polymorphic DNA (RAPD to distinguish among Scots pine populations from Serbia was evaluated. Sixteen arbitrary 10-mer primers employed in the analysis produced 54 fragments of which 21 were polymorphic (38.89%. Certain rare and genotype-specific bands were identified which could be effectively used to distinguish between the populations. Polymorphism in RAPD markers among P. sylvestris populations was high and sufficient to distinguish each of the populations. The results obtained suggest that RAPD markers are valuable for the genetic divergence estimation in Pinus sylvestris and for the study of divergence among populations.

  1. Soil Enzyme Activities in Pinus tabuliformis (Carriére) Plantations in Northern China

    OpenAIRE

    Weiwei Wang; Deborah Page-Dumroese; Ruiheng Lv; Chen Xiao; Guolei Li; Yong Liu

    2016-01-01

    Changes in forest stand structure may alter the activity of invertase, urease, catalase and phenol oxidase after thinning Pinus tabuliformis (Carriére) plantations in Yanqing County of Beijing, China. We examined changes in these soil enzymes as influenced by time since thinning (24, 32, and 40 years since thinning) for 3 seasons (spring, summer and autumn) following harvesting at two depths in the mineral soil (0–10 cm and 10–20 cm). Invertase and urease increased significantly with time sin...

  2. Ascensão capilar de água em substratos de coco e de pinus

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    Carlos Vinicius Garcia Barreto

    2012-01-01

    Full Text Available O uso da irrigação por capilaridade na produção de mudas em viveiros pode trazer vantagens ao uso de água e sua utilização adequada requer o conhecimento da capacidade de ascensão de água no meio de crescimento das raízes, atributo pouco estudado. Para avaliar esta condição fundamental, este trabalho tem como objetivo avaliar a ascensão de água em substratos comerciais de coco e pinus, em dois padrões de tamanho de partículas e em duas condições de umidade, para indicar aquele que possua as melhores características físicas para aplicação na irrigação por capilaridade. Utilizou-se o método de ascensão capilar, com colunas desmontáveis de anéis preenchidas com substratos, avaliando-se os seguintes tempos de contato do fundo da coluna com a lâmina de água: 0,25, 0,5, 1, 2, 4, 8, 16 e 24 horas, com 10 repetições por tempo testado. Pelos resultados, observou-se que a maior ascensão capilar acumulada em 24 horas de ensaio foi obtida para os substratos de textura fina de coco e de pinus. Além disso, a hidrofobia do substrato de coco seco recomenda que as irrigações nesse material devem ter frequências maiores que substratos de pinus, evitando seu secamento. O substrato de pinus, por não apresentar esse comportamento, deve ter turnos de rega mais espaçados, principalmente sob textura fina. Devido à sua maior retenção de água e capacidade de elevação de água quando umedecido, o substrato fino de coco é mais adequado à irrigação por capilaridade em recipientes como tubetes.

  3. RESISTENCIA A BAJAS TEMPERATURAS EN Pinus hartwegii SOMETIDO A DIFERENTES TRATAMIENTOS CON POTASIO

    OpenAIRE

    2010-01-01

    Con el fin de mejorar la resistencia a bajas temperaturas, se probó la aplicación de diferentes concentraciones de Nitrato de Potasio a plantas en vivero de 13 meses de edad de Pinus hartwegii Lindl. Se estableció un diseño experimental en bloques completos al azar, con cuatro repeticiones. Los tratamientos fueron cinco aplicaciones complementarias de 180, 150 y 114 ppm K, este último es la fertilización regular en el vivero forestal, durante la fase de endurecimiento. Dieciséis brinzales de ...

  4. DETERMINING THE TRANSITION AGE FROM JUVENILE TO MATURE WOOD IN Pinus patula SCHL. et CHAM.

    OpenAIRE

    David Josué Meza Juárez; J. Jesús Vargas Hernández; Javier López Upton; Humberto Vaquera Huerta; Amparo Borja de la Rosa

    2005-01-01

    In order to compare several criteria to estimate the transition age from juvenile to mature wood formation in a 16-year-old Pinus patula plantation, radial trends of latewood proportion, wood density and tracheid length were identified in a sample of 100 trees. A wood sample from each tree was taken with an increment borer at 50 cm stem height. Latewood proportion increased gradually from pith to bark, but after 14 years old showed a higher rate of increase; wood density decreased initially a...

  5. On the specific pattern of long chain polyprenols in green needles of Pinus mugo Turra.

    Science.gov (United States)

    Kazimierczak, B; Hertel, J; Swiezewska, E; Chojnacki, T; Marczewski, A

    1997-01-01

    In green needles of Pinus mugo the most abundant polyprenols occur as a mixture of prenologues in which the dominant alcohol is built of 16 isoprene units. The characteristic spectrum of polyprenols (prenol-15, -16 and -17) was the same irrespective of the location of plant and of distinct morphological differences observed in the various selected forms of this species. The constant pattern of the polyprenols spectrum was preserved throughout the 2-year life span of needles, although the level of polyprenols was increased 2-3-fold. The polyprenol pattern in Pinaceae family differs from species to species, thus it may serve as chemotaxonomic criterion within this systematic group.

  6. Gas chromatography-mass spectrometry study of sterols from Pinus elliotti tissues.

    Science.gov (United States)

    Laseter, J. L.; Evans, R.; Weete, J. D.; Walkinshaw, C. H.

    1973-01-01

    A comparative study of the sterol components of slash pine (Pinus elliotti) callus tissue cultures, seeds, and seedlings was carried out using GC-MS techniques. Cholesterol, desmosterol, campesterol, stigmasterol, sitosterol and cycloeucalenol were identified in all tissues while lophenol and 24-methylenelophenol were identified in only the seed and seedlings. 24-Ethylidenelophenol was detected in trace concentrations in only the seedlings. Sitosterol was the predominant sterol component, i.e., 80.8, 38.1 and 47.8% of the tissue culture, seed and seedling sterols, respectively.

  7. Essential oils composition of Pinus peuce Griseb. (Pinaceae growing on Pelister Mtn., Republic of Macedonia

    Directory of Open Access Journals (Sweden)

    Marija Karapandzova

    2011-06-01

    Full Text Available The composition of essential oils obtained by hydrodistillation from needles, from branches without needles and from branches with needles of Pinus peuce Griseb. (Pinaceae from Pelister Mtn. (R. Macedonia was analyzed by GC/FID/MS. One hundred and seven components (40 monoterpenes, 37 sesquiterpenes, 9 diterpenes and 21 other components - aliphatic and cyclic hydrocarbons; aliphatic alcohols, aldehydes, and acids; phenols and other oxygenated benzene derivates were identified. The most abundant constituents were terpene hydrocarbons, encompassing the monoterpenes: α-pinene, β-pinene, limonene + β-phellandrene and bornyl acetate and the sesquiterpenes: trans (E-caryophyllene and germacrene D.

  8. Ectomycorrhizal symbiosis in vitro between Tricholoma matsutake and Pinus densiflora seedlings that resembles naturally occurring 'shiro'.

    Science.gov (United States)

    Yamada, Akiyoshi; Maeda, Ken; Kobayashi, Hisayasu; Murata, Hitoshi

    2006-03-01

    We established an in vitro ectomycorrhizal symbiosis between Tricholoma matsutake and Pinus densiflora. Mycorrhiza formed in a substrate of Modified Norkrans' C medium and granite-based soil had features similar to those observed previously only in naturally occurring mycorrhizal system called 'shiro,' and promoted the growth of plants with smaller root/shoot ratios. The in vitro formation of 'shiro' is essential for the development of T. matsutake system to produce mushrooms and is useful for the propagation and plantation of the mycorrhizal seedlings.

  9. A Comparative Study on Turpentine Oils of Oleoresins of Pinus sylvestris L. from Three Districts of Denizli

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    İbrahim Tümen

    2010-10-01

    Full Text Available Oleoresin samples collected from Pinus sylvestris L. trees from Acıpayam, Çal and Çamlıbel, three different locations in Denizli-Turkey. The constituents of the turpentine oil, obtained by hydrodistillation of oleoresin of Pinus sylvestris L., were identified by GC-MS. Fifty four constituents were detected from the turpentine oil, which constituted about between 96.2% and 98.2% of the total amount. Major constituents of the oil were a-pinene, b-pinene, camphene, longifolene, D3-carene, limonene and b-caryophyllene

  10. Effects of light radiation intensity on photosynthetic characteristics and water use efficiency of Platycladus orientalis and Pinus tabulaeformis seedlings

    Institute of Scientific and Technical Information of China (English)

    ZHANG Weiqiang; HE Kangning; WANG Yunqi; WANG Baitian; DENG Juntao; ZHOU Yi; ZHONG Xijun; LI Zhaoqing

    2007-01-01

    In order to offer a scientific basis for cultivation and management of forests,effects of light radiation intensity on photosynthetic characteristics and water use efficiency of Platycladus orientalis and Pinus tabulaeformis were studied under different soil moisture contents.By adopting artificial control methods to soil moisture,and under simulated photosynthetic radiation (SPR),the net photosynthetic rate (PN),transpiration rate (Tr),water use efficiency (WUE) and intercellular CO2 concentration (Ci,) of Platycladus orientalis and Pinus tabulaeformis in the semi-arid region of the Loess Plateau,were studied.Results are as follows:within the photon range of 0-2,200 μmol/(m2·s),PN,Tr and WUE were enhanced with an increase in SPR in both species.PN and WUE of Platycladus orientalis and Pinus tabulaeformis,however,declined with continued increase in SPR.PN,Tr,WUE and light saturation point (LSP) of Platycladus orientalis were higher than those of Pinus tabulaeformis,while light compensation point (LCP) of Platycladus orientalis was lower than that of Pinus tabulaeformis at the same soil moisture content.The efficiency of light energy utilization of Platycladus orientalis was higher than that of Pinus tabulaeformis;PN,Tr and Ci of Platycladus orientalis and Pinus tabulaeformis were enhanced by increasing soil moisture content,whereas WUE declined.At soil moistures of 7.90%,13.00% and 19.99%,LSP of Platycladus orientalis LCP was 42,25 and 13 μmol/(m2·s) respectively,with corresponding maximal net CO2 photosynthetic rates (Pmax)of 3.04,4.06 and 5.53 μmol(m2·s).At soil moistures of 7.83%,13.04% and 20.15%, the LSP of Pinus tabulaeformis was 1,100, 1,325 and 1,500 μmol/(m2·s) respectively and LCP was 60,30 and 23μmol/(m2·s), with Pmas of 1.08, 3.35 and 4.36 μmol/(m2·s)respectively.

  11. “El libro que contiene onze partidos del M. Dn Joseph de Torres”. ¿Torres y Martínez Bravo, o Torres y Vergara? Aproximaciones a una interpretación filológica del manuscrito de la colección Sánchez Garza

    Directory of Open Access Journals (Sweden)

    Delgado Parra, Gustavo

    2008-12-01

    Full Text Available The Torres Book from the Sanchez Garza collection is one of the most polemic issues in the study of Hispanic music. This controversy has been the result of two main factors. On the one hand, the authorship problems and on the other, the apparent disorder of the musical content in this manuscript due to the ineffi cient work done by the copiers who gave it form. The latter has lead to conclude, as a generalized opinion, that it is a highly corrupted copy of the organ works that could have been composed by the famous Spanish composer, organist, musical theorist and printer, Joseph de Torres y Martinez Bravo (*1670c; †1738; in which case the copier would be the one to blame for the demerit of such a renowned musician of the Royal Chapel of Madrid. If to this we add the transcription offered by the CENIDIM in 1993 which multiplies the confusion, we are facing pieces of music full of questions and a musical text which is neither effective nor convincing.El Libro de Torres de la colección Sánchez Garza, es uno de los documentos más polémicos de los últimos tiempos en la musicología hispánica. Esta polémica ha sido el resultado de dos factores fundamentales: por un lado el problema de la autoría, y por el otro, el aparente desorden del contenido musical del manuscrito, debido supuestamente, al trabajo inefi ciente de los copistas que le dieron forma. Esto último ha llevado a concluir, como opinión generalizada, que se trata de una copia corrompida de las obras para órgano que habrían sido compuestas por el prestigiado compositor, organista, teórico musical e impresor español, Joseph de Torres y Martínez Bravo (*1670c; †1738, en cuyo caso el copista del manuscrito sería el responsable de dicho desorden; lo que ha llevado a suponer, a otros investigadores, que incluso las obras en cuestión no sean de su autoría. A esto debemos agregar la confusa y a veces errónea transcripción ofrecida por el CENIDIM en 1993, en cuyo caso las

  12. Short Communication. Physiological effects of Rhizopogon Roseolus on Pinus halepensis seedlings

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    J.A. Alfonso Domínguez Núñez

    2013-12-01

    Full Text Available Aim of study: The inoculation of forest seedlings with ectomycorrhizal fungi can improve the morphological and physiological qualities of plants, especially those used for regeneration of arid areas. Rhizopogon roseolus is an ectomycorrhizal fungus (ECM commonly used for reforestation. In this study, the specific objectives were to know some morphophysiological effects of Rhizopogon Roseolus on Pinus halepensis seedlings under standard nursery conditionsArea of study: ETSI Montes and EUIT Forestal, Madrid.Material and Methods: In nursery, under well watered conditions and peat growing substrates, Aleppo pine seedlings were inoculated with R. roseolus. Five months after the inoculations, we examined the growth, water parameters (osmotic potential at full turgor [Ψπfull], osmotic potential at zero turgor [Ψπ0], and the tissue modulus of elasticity near full turgor [Emax], mycorrhizal colonization, and concentration and content of macronutrients in the seedlings. Subsequently, a trial was conducted to assess the root growth potential.Main results: The mycorrhization decreased the height and diameter of mycorrhizal seedlings but increased the root weight and root branching. R. roseolus did not cause any significant effect on the regeneration of new roots or on any of the tested hydric parameters, but it did improve N uptake of the seedlings.Research highlights: The mycorrhizal inoculation increased the N uptake. The mycorrhizal inoculation caused opposite effects on some growth parametersKeywords: Osmotic adjustment; elastic adjustment; mineral nutrition; root growth potential; nursery; Rhizopogon roseolus;  Pinus halepensis. 

  13. Photosynthetic response of Pinus sylvestriformis to elevated carbon dioxide and its influential factor analysis

    Institute of Scientific and Technical Information of China (English)

    2000-01-01

    The photosynthetic response of 12-year old Pinus sylvestriformis to elevated CO2 and its influential factors were tested and analyzed in the forest region of Changbai Mountain in 1999. Trees grown at the natural condition were controlled at three levels of CO2 concentration (350 μL.L-1·, 500 μL.L -1 and 700 μL.L-1) by CO2 rich settlement designed by us. Net photosynthetic rates (NPR), temperature, relative humidity, stomatal conductance, intercellular CO2 concentration and photosynthetic active radiation (PAR) were measured at 6:00, 8:00, 10:00, 14:00, 16:00 and 18:00 hours a day. Experimental results showed that the NPR of Pinus sylvestriformis increased by 32.6% and 123.0% at 500 μL.L-1 and 700 μL.L-1 CO2 concentration respectively, compared to ambient atmospheric CO2 concentration (350 μL.L-1). The relations between NPR and influential factors, including temperature, relative humidity, intercellular CO2 concentration and photosynthetic active radiation, were analyzed respectively by regression analysis at different CO2 concentrations.

  14. Ecophysiological responses of Pinus leucodermis at high elevation in the Mediterranean area

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    Guerrieri MR

    2008-03-01

    Full Text Available Pinus leucodermis Antoine (= Pinus heldreichii var. leucodermis is a species of the Balkan flora which in Italy grows in a small area between the Regions of Basilicata and Calabria, in the Pollino National Park. A relict of the oro-Mediterranean Tertiary forests, it is found from the lower vegetation belt, where it is mixed with evergreen sclerophyllous vegetation, up to the alpine vegetation belt beyond the closed formations of Fagus sylvatica, where it makes up stands with very old, isolated and big trees growing in open spaces between rocks. The ecophysiological and dendrochronolgy features of the P. leucodermis stands growing on the west slope of Serra di Crispo (Monte Pollino, between 1800 and 2000 m a.s.l., have been studied during recent years and some of the results are presented in this work. Ecophysiological measurements in situ show that reduced atmospheric vapour pressure deficit conditions, due to humid currents from the western Tyrrhenian Sea, allow the trees to escape from summer drought. When day summer weather conditions are optimal P. leucodermis exhibits a remarkable photosynthetic activity and adaptation to high irradiance. The tree ring width chronology documents a marked reduction of radial growth from 1950 to 1985, followed by a strong recovery, still continuing. In the same period differences between the annual minimum and maximum temperatures tend to increase. Anthropic and grazing pressure is reduced markedly after the institution of the National Park of Pollino.

  15. Abundance, diversity, and vitality of mycorrhizae of Scots pine (Pinus sylvestris L.) in lignite recultivation sites.

    Science.gov (United States)

    Münzenberger, B; Golldack, J; Ullrich, A; Schmincke, B; Hüttl, R F

    2004-07-01

    Scots pine (Pinus sylvestris L.) stands cover large areas in the Lusatian and the Middle German lignite mining districts. Due to adverse chemical substrate conditions, the root systems of the trees are restricted to the ameliorated top-spoil and the organic forest floor layers. To investigate functioning of fine root systems under the prevailing site factors, we studied mycorrhizal colonization rate and frequency as well as mycorrhizal diversity, vitality and growth phases in Scots pine ecosystems along a chronosequence in both mining districts. Mycorrhizal rate was close to 100% in both districts. Mycorrhizal abundance was higher in the organic forest floor layer than the mineral soil layer. In total, 25 morphotypes were recorded. Diversity differed between the districts. The mycorrhizae of Amphinema byssoides, Tuber puberulum, Pinirhiza discolor, Pinirhiza cf. bicolorata and E-type were present in both mining areas. These morphotypes are typical of nutrient-rich soils with high pH values. Compared with the undisturbed sites, vitality of mycorrhizae was very high at the test sites on spoil substrate, correlating with the high growth dynamics of mycorrhizae at recultivation sites. A relatively high carbon flow to the mycorrhizal root systems at these sites seems likely. Thus, mycorrhizal root systems are able to cope with the ameliorated top-spoil and the organic layer. The main reason for the adaptation is the large number of ectomycorrhizal fungal species available in this area where Pinus sylvestris is indigenous.

  16. Growth and Survival Variation among Scots Pine (Pinus sylvestris L.) Provenances.

    Science.gov (United States)

    Gülcü, Süleyman; Bilir, Nebi

    2017-01-01

    Tree height, basal diameter, and survival were examined in thirteen-year-old provenance test established by 30 seed sources of Scots pine (Pinus sylvestris L.) at two exotic sites of the species in Southern part of Turkey. Variations within provenance and among provenances and relations among the traits were estimated to compare Scots pine provenance and two other native species. Averages of tree height and basal diameter were 350 cm and 52.7 mm in Aydogmus site and 385 cm and 51.2 mm in Kemer site, respectively. There were large differences within and among provenances for the characters. Sites were similar (p > 0.05) for the characters, while there were significant differences (p ≤ 0.05) among provenances within site according to results of variance analysis (ANOVA). Scots pine provenances were higher and had more thickness than that of black pine (Pinus nigra Arnold) and Taurus cedar (Cedrus libani A. Rich.) which were natural species of the region. There were positive and significant (p pine and 53% in Taurus cedar for the sites respectively.

  17. Interpopulation genetic-ecological variation of Scots pine (Pinus sylvestris L. in Serbia

    Directory of Open Access Journals (Sweden)

    Lučić Aleksandar

    2011-01-01

    Full Text Available The genetic-ecological variation of Scots pine (Pinus sylvestris L. in Serbia was studied in the populations at five localities in western and south-western Serbia. Three groups of Scots pine (Pinus sylvestris L. populations were differentiated based on genetic research (seed protein analysis and plant community research. The first group consists of Scots pine populations on Šargan (FMU “Šargan“ and on Tara (FMU “Kaluderske Bare”, where the forests belong to the community of Scots pine and Austrian pine (Pinetum sylvestris-nigrae Pavlovic 1951. The second group covers the localities Stolovi (FMU “Radocelo-Crepuljnik“ and Zlatar (FMU “Zlatar I“, where the forests belong to the community of Scots pine and spruce (Piceo abietis-Pinetum sylvestris Stefanovic 1960. The third group comprises the Scots pine population on Pešter (FMU “Dubocica-Bare“ which belongs to the community of Scots pine with erica (Erico-Pinetum sylvestris Stefanovic 1963. Cluster analysis was performed on the basis of seed protein data and showed that there are three groups of Scots pine populations. The three populations coincide with plant communities. The community of Scots pine with erica (Erico-Pinetum sylvestris Stefanovic 1963 recorded on Pešter at the locality “Dubocica- Bare“ in the area of FE “Golija“ Ivanjica, is a special Scots pine population displayed at the greatest distance from all other populations in the cluster analysis dendrogram.

  18. High seed dispersal ability of Pinus canariensis in stands of contrasting density inferred from genotypic data

    Directory of Open Access Journals (Sweden)

    Unai López de Heredia

    2015-04-01

    Full Text Available Aim of the study: Models that combine parentage analysis from molecular data with spatial information of seeds and seedlings provide a framework to describe and identify the factors involved in seed dispersal and recruitment of forest species. In the present study we used a spatially explicit method (the gene shadow model in order to assess primary and effective dispersal in Pinus canariensis. Area of study: Pinus canariensis is endemic to the Canary Islands (Spain. Sampling sites were a high density forest in southern slopes of Tenerife and a low density stand in South Gran Canaria. Materials and methods: We fitted models based on parentage analysis from seeds and seedlings collected in two sites with contrasting stand density, and then compared the resulting dispersal distributions. Main results: The results showed that: 1 P. canariensis has a remarkable dispersal ability compared to other pine species; 2 there is no discordance between primary and effective dispersals, suggesting limited secondary dispersal by animals and lack of Janzen-Connell effect; and 3 low stand densities enhance the extent of seed dispersal, which was higher in the low density stand. Research highlights: The efficient dispersal mechanism of P. canariensis by wind inferred by the gene shadow model is congruent with indirect measures of gene flow, and has utility in reconstructing past demographic events and in predicting future distribution ranges for the species.

  19. Biogeochemical assessment of environmental risk by the example of Pinus sylvestris L.

    Directory of Open Access Journals (Sweden)

    Rybakov Dmitry Sergeevich

    2016-06-01

    Full Text Available The possibility of using annual rings of Pinus sylvestris L. to assess chemical pollution in the Republic of Karelia is discussed. With the help of principal component factor analysis the relationship between the contents of chemical elements and dendrological parameters – the width of annual rings and wood density - was revealed. The main factors contributing to the pollution of the northern part of the Republic of Karelia by the emission from iron-ore enterprise JSC «Karelsky Okatysh» are frequent ecologically significant wind directions, the distance from the source and the total mass of the emission. The value of radial growth (the average width of annual rings for five-years of Pinus sylvestris L. is connected negatively with the content of Cu, Cd, Ni and Fe. As an element of nutrition, Mn is positively correlated with the density of wood wherein it is an antagonist of Fe and partially of Cr in northern areas . Several peaks of Pb content coincide with the most intense tests of atomic weapons. Sharp maximums of Pb can also be due to a cross-border pollution and emission from regional sources.

  20. Development and Characterisation of Irap Markers From Expressed Retrotransposon-like sequences in Pinus sylvestris L.

    Directory of Open Access Journals (Sweden)

    Voronova Angelika

    2014-07-01

    Full Text Available Conifer genomes are large and stably diploid, in contrast to angiosperms, which are more variable both in genome size and ploidy. Conifer genomes are characterised by multiple gene families and pseudogenes, contain large inter-gene regions and a considerable proportion of repetitive sequences. All members of plant retrotransposon orders have been identified in gymnosperm genomes, however active elements have not been described. Investigation of transposable elements in Scots pine (Pinus sylvestris L. could offer insights into transposon-mediated reorganisation under stress conditions in complex and ancient plant genomes. Nine Pinus sylvestris specific markers were developed to hypothetical long terminal repeats (LTRs from differentially expressed retrotransposon-like fragments after heat stress and insect damage. Genetic diversity of 150 trees from a naturally regenerated pine stand was investigated using the IRAP method. The developed markers revealed high levels of genetic diversity and were able to distinguish subpopulations growing in long-term differential environmental conditions. Somaclonal variation was also investigated using these markers and polymorphic fragments were identified between ramets of Scots pine clones growing in two different plantations, possibly indicating evidence of recent transposition events. Sequencing of the polymorphic fragments identified two groups of sequences containing LTR sequences of an unknown retrotransposon with homology to the LTRs of the Copia-17-PAb-I element.

  1. Rapid changes in induced non-volatile secondary metabolites in damaged Pinus massoniana Lamb.

    Institute of Scientific and Technical Information of China (English)

    Qin REN; Yongjian HU; Youju JIN; Wenhong DENG; Zhenyu LI; Li YANG; Mwange Kalima NKOMA

    2008-01-01

    Plants initiate the development of defense mechanisms as soon as pests start to cause damage to them. In order to have a thorough understanding of the physiological mechanisms of the Pinus massoniana self-defense mechanism, and to provide a theoretical founda-tion for an effective ecological management of this plant, levels of tannin, polyamine and phenolic acids were ana-lyzed in undamaged (UDL), insect-damaged (IDL) and artificially-damaged (ADL) leaves at different times. Results show that, although the content of tannin signifi-cantly increased in IDL and ADL compared to UDL, its peaks appeared earlier in ADL than in IDL treatment. Tannin concentration substantially increased again 48 h after IDL treatment. On the other hand, the damage mode considerably affected putrescine and spermidine levels in leaves. Their concentrations in IDL plants remained higher than in UDL after a relatively long time (72 h), but spermine was barely detected in any of the samples. In general, total content of phenol acids significantly increased in damaged leaf treatments (ADL and IDL), with a higher level in IDL for most of the investigated phenolic acids, except for ferulic acid. Our study showed that, when damaged by insects, Pinus massoniana rapidly produces substances required in resistance induction to insects in order to insure its self-protection.

  2. Photosynthesis, chloroplast pigments, and antioxidants in Pinus canariensis under free-air ozone fumigation

    Energy Technology Data Exchange (ETDEWEB)

    Then, Ch. [Federal Research and Training Centre for Forests, Natural Hazards and Landscape, Unit of Alpine Timberline Ecophysiology, Rennweg 1, A-6020 Innsbruck (Austria); Ecophysiology of Plants, Department of Ecology, Technische Universitaet Muenchen, Life Science Center Weihenstephan, Am Hochanger 13, D-85354 Freising (Germany); Herbinger, K. [Institute for Plant Sciences, University of Graz, Schubertstr. 51, A-8010 Graz (Austria); Luis, V.C. [Fundacion Centro de Estudios Ambientales del Mediterraneo (CEAM) Dpto, Restauracion Forestal, University of Alicante, Department of Ecology, Faculty of Sciences, Ap 99, Ctra. San Vicente del Raspeig s/n, San Vicente del Raspeig 03690, Alicante (Spain); Heerdt, C. [Ecoclimatology, Am Hochanger 13, D-85354 Freising (Germany); Matyssek, R. [Ecophysiology of Plants, Department of Ecology, Technische Universitaet Muenchen, Life Science Center Weihenstephan, Am Hochanger 13, D-85354 Freising (Germany); Wieser, G. [Federal Research and Training Centre for Forests, Natural Hazards and Landscape, Unit of Alpine Timberline Ecophysiology, Rennweg 1, A-6020 Innsbruck (Austria)], E-mail: gerhard.wieser@uibk.ac.at

    2009-02-15

    High O{sub 3} levels, driving uptake and challenging defense, prevail on the Canary Islands, being associated with the hot and dry summers of the Mediterranean-type climate. Pinus canariensis is an endemic conifer species that forms forests across these islands. We investigated the effects of ozone on photosynthesis and biochemical parameters of P. canariensis seedlings exposed to free-air O{sub 3} fumigation at Kranzberg Forest, Germany, where ambient O{sub 3} levels were similar to those at forest sites in the Canary Islands. The twice-ambient O{sub 3} regime (2xO{sub 3}) neither caused visible injury-like chlorotic or necrotic spots in the needles nor significantly affected violaxanthin, antheraxanthin and zeaxanthin levels and the de-epoxidation state of the xanthophyll cycle. In parallel, stomatal conductance for water vapour, net photosynthesis, intercellular CO{sub 2} concentration, chlorophyll fluorescence parameters, as well as antioxidant levels were hardly affected. It is concluded that presently prevailing O{sub 3} levels do not impose severe stress on P. canariensis seedlings. - Twice-ambient ozone does not significantly affect the physiological behavior of Pinus canariensis seedlings.

  3. Molecular characterization of zeta class glutathione S-transferases from Pinus brutia Ten.

    Indian Academy of Sciences (India)

    E. Oztetik; F. Kockar; M. Alper; M. Iscan

    2015-09-01

    Glutathione transferases (GSTs; EC 2.5.1.18) play important roles in stress tolerance and metabolic detoxification in plants. In higher plants, studies on GSTs have focussed largely on agricultural plants. There is restricted information about molecular characterization of GSTs in gymnosperms. To date, only tau class GST enzymes have been characterized from some pinus species. For the first time, the present study reports cloning and molecular characterization of two zeta class GST genes, namely PbGSTZ1 and PbGSTZ2 from Pinus brutia Ten., which is an economically important pine native to the eastern Mediterranean region and have to cope with several environmental stress conditions. The PbGSTZ1 gene was isolated from cDNA, whereas PbGSTZ2 was isolated from genomic DNA. Sequence analysis of PbGSTZ1 and PbGSTZ2 revealed the presence of an open reading frame of 226 amino acids with typical consensus sequences of the zeta class plant GSTs. Protein and secondary structure prediction analysis of two zeta class PbGSTZs have shared common features of other plant zeta class GSTs. Genomic clone, PbGSTZ2 gene, is unexpectedly intronless. Extensive sequence analysis of PbGSTZ2, with cDNA clone, PbGSTZ1, revealed 87% identity at nucleotide and 81% identity at amino acid levels with 41 amino acids differences suggesting that genomic PbGSTZ2 gene might be an allelic or a paralogue version of PbGSTZ1.

  4. Energy characterization of fresh and torrified pellets produced from Pinus waste wood

    Directory of Open Access Journals (Sweden)

    Thiago de Paula Protásio

    2015-12-01

    Full Text Available he objective of this research was to evaluate torrified and fresh pellets produced from Pinus waste wood, used for direct generation of thermal energy. The compaction of Pinus sp. waste wood from lumbermill was performed in a pelletizer with a planar array of 8 mm. Roastings were performed in an electric furnace, type muffle, stabilized at final temperatures of 220 °C and 250 °C for 30 min. The following pellets properties were determined: apparent and unit energy density, bulk density, energy bulk density, immediate chemical composition (volatile materials, ash and fixed carbon, high heating value and moisture. It was observed an increase in high heating value and reduction of moisture content of torrified pellets. However, the gain in calorific value was less than the mass loss of the pellets after roasting, reducing the energy densities of the pellets. The pellets raw have marketing potential in European countries such as Germany, Austria and Sweden. The methodology used for roasting is not suitable for pellets heat treatment. However, further research on pellet roasting in a wider temperature and residence time range is recommended, in order to define parameters that optimize their energetic properties.

  5. [Spectral features analysis of Pinus massoniana with pest of Dendrolimus punctatus Walker and levels detection].

    Science.gov (United States)

    Xu, Zhang-Hua; Liu, Jian; Yu, Kun-Yong; Gong, Cong-Hong; Xie, Wan-Jun; Tang, Meng-Ya; Lai, Ri-Wen; Li, Zeng-Lu

    2013-02-01

    Taking 51 field measured hyperspectral data with different pest levels in Yanping, Fujian Province as objects, the spectral reflectance and first derivative features of 4 levels of healthy, mild, moderate and severe insect pest were analyzed. On the basis of 7 detecting parameters construction, the pest level detecting models were built. The results showed that (1) the spectral reflectance of Pinus massoniana with pests were significantly lower than that of healthy state, and the higher the pest level, the lower the reflectance; (2) with the increase in pest level, the spectral reflectance curves' "green peak" and "red valley" of Pinus massoniana gradually disappeared, and the red edge was leveleds (3) the pest led to spectral "green peak" red shift, red edge position blue shift, but the changes in "red valley" and near-infrared position were complicated; (4) CARI, RES, REA and REDVI were highly relevant to pest levels, and the correlations between REP, RERVI, RENDVI and pest level were weak; (5) the multiple linear regression model with the variables of the 7 detection parameters could effectively detect the pest levels of Dendrolimus punctatus Walker, with both the estimation rate and accuracy above 0.85.

  6. Liming and fertilisation in Pinus taeda plantations with severe nutrient deficiency in savanna soils

    Directory of Open Access Journals (Sweden)

    Araína Hulmann Batista

    2014-11-01

    Full Text Available Soils with high acidity and low exchangeable bases may be responsible for low yields of Pinus taeda in a forest plantation at Jaguariaíva, Paraná State, Brazil. The aim of this study was to evaluate the effect of liming and fertilisation, applied over litter, on two selected areas with Pinus taeda plantations. Soil, litter and pine needles were evaluated for K, Ca and Mg concentrations and soil acidity parameters. Seven treatments were applied: (i complete (N, P, K, Zn, Cu, B, Mo, and lime; (ii without N, P, and K; (iii without Zn, Cu, B, and Mo; (iv without K; (v without Zn; (vi without lime; and (vii control (without nutrients and lime. Soil samples were collected at five soil depths (0-5, 5-10, 10-20, 20-40 and 40-60 cm simultaneously with litter samples. Needles were also collected from the first and second pine flushes. Liming induced soil pH, Ca2+, and Mg2+ increases, and the opposite was observed for Al3+ and Al saturation. Fertilisation increased soil exchangeable K+ concentrations and needle and litter K concentrations. The low Ca and Mg concentrations found in the plant needles might be attributable to their low mobility.

  7. Using Landsat satellite imagery to detect small-size forest stands of Pinus nigra Arn. and Pinus sylvestris L. affected by Scolytidae; Uso de imagenes satelite Landsat para la deteccion de rodales de Pinus nigra Arn. y Pinus sylvestris L. afectados por escolitidos

    Energy Technology Data Exchange (ETDEWEB)

    Blanco, E.; Bonet, J. A.; Eizaguirre, M.

    2009-07-01

    Medium resolution images from multispectral sensors like Landsat TM have been extensively used for decades in order to identify decline and defoliation generated by insects and other forest pests. The present work analyses the usefulness of these kinds of images to detect small-size stands of Pinus nigra Arn. and Pinus sylvestris L. affected by Scolytidae attacks. The study area was located in the Solsones region (Eastern Pyrenees), selecting 34 training zones (17 damaged small-size stands and 17 healthy small-size stands). The exploratory analysis of the images was conducted with the ERDAS IMAGINE 8.x. program.The results of the study showed significant differences between the affected and non-affected stands in 5 of the 7 spectral bands analysed. TM5 and TM7 bands were identified as those having the highest power to detect damaged stands. The digital levels obtained and the spaces of characteristics created, both showed trends to group small-size affected stands versus healthy, achieving improvements in the methodological procedure employed. (Author) 31 refs.

  8. Catalytic Conversion of Pinus densiflora Over Mesoporous Catalysts Using Pyrolysis Process.

    Science.gov (United States)

    Joo, Sung Kyun; Lee, In-Gu; Lee, Hyung Won; Chea, Kwang-Seok; Jo, Tae Su; Jung, Sang-Chul; Kim, Sang Chai; Ko, Chang Hyun; Park, Young-Kwon

    2016-02-01

    Catalytic pyrolysis experiments were conducted to investigate the possibility of obtaining valuable chemicals from Pinus densiflora, a native Korean tree species occupying 21.4% of the total area under forests in South Korea. Two representative mesoporous catalysts, Al-MCM-41 and Al-MSU-F, as well as hierarchical mesoporous MFI (Meso-MFI) that has both mesopores and micropores, were used as catalysts. Compared to non-catalytic pyrolysis, catalytic pyrolysis was shown to reduce the fractions of levoglucosan, phenolics, and acids in bio-oil, while increasing the fractions of aromatics, PAHs, and furans. Meso-MFI with strong acid sites showed a high selectivity toward aromatics and PAHs, whereas Al-MCM-41 and Al-MSU-F with weak acid sites exhibited a high selectivity toward furanic compounds. The results of this study indicate that choosing a catalyst with an adequate quantity of acidic sites with the required strength is critical for enhancing the production of desired chemicals from Pinus densiflora.

  9. Antioxidant Activity and Protection from DNA Damage by Water Extract from Pine (Pinus densiflora) Bark.

    Science.gov (United States)

    Jiang, Yunyao; Han, Woong; Shen, Ting; Wang, Myeong-Hyeon

    2012-06-01

    Water extract from Pinus densiflora (WPD) was investigated for its antioxidant activity and its ability to provide protection from DNA damage. A series of antioxidant assays, including a 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical-scavenging assay, a reducing power assay, a metal-chelating assay, a superoxide radical scavenging assay, and a nitrite scavenging ability, as well as a DNA damage protection assay were performed. Total phenolic content was found to be 211.32 mg Tan/g WPD. The extract scavenged 50% DPPH free radical at a concentration of 21.35 μg/mL. At that same concentration, the reducing power ability of WPD was higher than that of α-tocopherol. The extract chelated 68.9% ferrous ion at the concentration of 4 mg/mL. WPD showed better nitrite scavenging effect at the lower pH. Meanwhile, WPD exhibited a strong capability for DNA damage protection at 1 mg/mL concentration. Taken together, these data suggest water extract from Pinus densiflora could be used as a suitable natural antioxidant.

  10. Bio-ecological Peculiarities of Genus Pinus L. Species Under Conditions of Eastern Georgia

    Directory of Open Access Journals (Sweden)

    Tamar Nadiradze

    2014-06-01

    Full Text Available The article discusses bio-ecological peculiarities of the following species of genus Pinus L. spread in eastern Georgia: Pinus eldarica Medw., P. griffithii McCleland., P. cembra L., P. pallasiana Lamb., P. pinea L., P. sabiniana Dougl., P. sylvestris L., P. sosnowskyi Nakai, P. strobus L., the perspectives of their usage in Green Building and contemporary problems. We have studied the periods of bud opening, vegetation ending, starting and finishing of cambium action, sprout woodening process, time and rate of growing in height, and regularities of accumulation-transformation of storage carbohydrates. The studies revealed that the annual development cycle of all these species includes all morphological -physiological periods: sprout growth, latent growth, organic and forced rest. They are characterized by the good growth-development; almost all of them are perspective for eastern Georgia, particularly, for all regions of inner Kakheti. However, in recent years, massive drying up of pine forests groves takes place in eastern Georgia. That is why it is necessary to conduct the fitopatologic research, identify the pest causing the damage and plan the measures against it.

  11. Establishment of Pinus halepensis Mill. saplings following fire: effects of competition with shrub species

    Science.gov (United States)

    De las Heras, J.; Martínez-Sánchez, J. J.; González-Ochoa, A. I.; Ferrandis, P.; Herranz, J. M.

    2002-05-01

    An early study analysing the effects of competition from Cistus monspeliensis-dominated shrub canopy on Pinus halepensis saplings, both colonising a recently burnt area, has been extended in order to test initial predictions. Inter-specific competition effects on P. halepensis were experimentally analysed by a shrub thinning-out treatment carried out 1 year after fire. The extension of the recorded period confirmed (i) a significant increase in height, and (ii) the lack of variation in density of P. halepensis saplings when the shrub layer was removed. In contrast, the increase in relative growth rate in height (RGRh) and the decrease in mortality recorded during early post-fire stages for treated units did not persist in subsequent years. These two treatment-induced effects disappeared 1 year after the shrub clearing (29 months after fire). It is hypothesised that this time should represent a culminating point in the inter-specific competition established between Cistus and Pinus saplings simultaneously colonising recently disturbed areas and be a critical period for pine sapling survival. After this time, a reduction in relatively short-lived Cistus populations and an increase in P. halepensis abundance should be expected in the community. It is concluded that a shrub-clearing treatment could be recommendable if the initial post-fire pine sapling density is not high enough to successfully face the early critical competitive period. Consequences of early shrub competition on forest productivity during mature phases are also discussed.

  12. ORIGEM DOS COMPOSTOS HIDROFÓBICOS E SEUS EFEITOS EM FLORESTAS DE Pinus E Eucalyptus

    Directory of Open Access Journals (Sweden)

    Eduardo Saldanha Vogelmann

    2015-01-01

    Full Text Available Hydrophobicity can be understood as the soil water repellency or difficulty to soil wetting. This is associated with the coating of soil particles by hydrophobic organic substances and currently there are numerous reports of water repellency in forest areas of Eucalyptus and Pinus. Thus, this study aims at reviewing and listing the aspects related to the origin of hydrophobic compounds and the effects of the water dynamics in the forest environment, as well as implications in growth and development of trees in Eucalyptus and Pinus forests. It is recognized that the local vegetation and some bacteria and fungi, depending on their chemical composition, may release hydrophobic organic substances. Another risk factor is the occurrence of burnings which induce changes in organic compounds and promote soil drying. Furthermore, the particle size distribution and the soil pH may be indirectly associated with the occurrence of soil water repellency The occurrence of hydrophobicity requires special attention regarding the soil use and management, because this repellency affects the water movement on the surface and inside the soil profile. Changes in sorptivity, reduction in water infiltration, increase in runoff and occurrence of preferential flow are the main hydrological effects of this phenomenon. Due to their lower infiltration rates there is increased the surface flow and reducing the amount of water available, increasing the erosion and, consequently affecting the growth and development of plants.

  13. Biomass and carbon stock from Pinus caribaea var. hondurensis under homogenous stands in southwest Bahia, Brazil

    Directory of Open Access Journals (Sweden)

    Máida Cynthia Duca de Lima

    2016-06-01

    Full Text Available ABSTRACT: There is a large number of studies evaluating methods to quantify biomass for the genus Pinus in different regions of Brazil. However, knowledge about this subject in the Northeast region of Brazil is still incipient. The objective of the present study was to assess the biomass and carbon stocks and select mathematical models to estimate these variables in Pinus caribaea var. hondurensis , which is established in homogenous stands in the Southwest region of the state of Bahia (Brazil. The biomass was quantified using the destructive method. Samples of needles, bole, bark, and branches were analyzed to determine their carbon contents. Ten models were tested, and the best of them were chosen based on the following statistical indicators: adjusted determination coefficient, estimate standard error, maximum likelihood logarithm, and graphical analysis of waste. Values for total biomass and carbon stocks were 69 and 42Mg ha-1, respectively. Curtis and Schumacher-Hall Log models showed to be the most indicated to estimate the total dry biomass and carbon of the species under the conditions studied.

  14. Effect of two successive wildfires in Pinus halepensis stands of central Greece

    Directory of Open Access Journals (Sweden)

    Y. Spanos

    2008-05-01

    Full Text Available We estimated differences, five years after a wildfire, in soil and vegetation between Pinus halepensis stands that were once burnt (1998 and stands that were burnt twice in a short time interval (1995 and 1998, in the area of Penteli, central Greece. The parameters monitored were the physical and chemical attributes of upper soil layer and the vegetation composition, density and height. The results showed that five years after the wildfire, soil pH did not differ between areas burnt once and twice, while the organic matter was higher in the once-burnt areas. The vegetation composition was similar in the two areas and the dominant species were those pre-existing the fire. On the contrary, vegetation density was considerably lower in the twice-burnt areas. The height of woody species oscillated in the same levels in the two areas. The plant community was composed mainly by resprouting species, like Quercus coccifera, Pistacia lentiscus, or Phillyrea latifolia, and less individuals from seed-regenerated species, like Pinus halepensis, Cistus monspeliensis, and C. creticus. It is suggested that the recurrence of wildfire affected negatively the ecosystem attributes, and contributed to the increased risk of degradation.

  15. Orthogonal cutting forces in juvenile and mature Pinus taeda wood Forças de corte ortogonal na madeira juvenil e adulta de Pinus taeda

    Directory of Open Access Journals (Sweden)

    Raquel Gonçalves

    2005-08-01

    Full Text Available The distinct characteristics of juvenile and mature woods, which are observed particularly in softwoods, have an influence on processing due to their different mechanical resistance properties in relation to cutting operations. In the past, when most of the wood used industrially came from adult trees of natural forests, little importance was given to a distinction between different zones of the tree stem. At present, however, as the supply of mature trees with large diameters from native forests is constantly decreasing, the use of short-cycle trees has become a common practice, through the adoption of species that grow relatively fast, such as pines and eucalyptus. In both softwoods and hardwoods, juvenile wood cells are generally smaller and thinner than in mature wood, and this reflects on their density and mechanical resistance, which should have an effect on the cutting forces developed during processing. The main object of this research was to evaluate orthogonal cutting forces in juvenile and mature Pinus taeda woods. Cutting force magnitude differences were observed for those two regions of the trunk, with parallel cutting forces being 33.4% higher, on average, at the mature wood region for 90-0 cutting, and 12% higher for 90-90 cutting. This result is consistent with the distinct anatomical structures of the material, since the forces developed during machining depend directly upon its properties.As características distintas dos lenhos juvenil e adulto, existentes principalmente na formação das coníferas, influenciam na usinagem devido às diferentes propriedades de resistência mecânica ao corte. No passado, quando a maior parte da madeira utilizada industrialmente era proveniente de árvores adultas de florestas naturais, pouca importância era dada à diferenciação de zonas no tronco. Atualmente, no entanto, com o decréscimo constante do suprimento de árvores adultas de grandes diâmetros, provenientes de florestas nativas

  16. Response of needle dark respiration of Pinus koraiensis and Pinus sylvestriformis to elevated CO2 concentra-tions for four growing seasons’ exposure

    Institute of Scientific and Technical Information of China (English)

    2007-01-01

    The long-term effect of elevated CO2 concentrations on needle dark respiration of two coniferous spe- cies—Pinus koraiensis and Pinus sylvestriformis on the Changbai Mountain was investigated using open-top chambers. P. koraiensis and P. sylvestriformis were exposed to 700, 500 μmol·mol-1 CO2 and ambient CO2 (approx. 350 μmol·mol-1) for four growing seasons. Needle dark respiration was meas- ured during the second, third and fourth growing seasons’ exposure to elevated CO2. The results showed that needle dark respiration rate increased for P. koraiensis and P. sylvestriformis grown at elevated CO2 concentrations during the second growing season, could be attributed to the change of carbohydrate and/or nitrogen content of needles. Needle dark respiration of P. koraiensis was stimu- lated and that of P. sylvestriformis was inhibited by elevated CO2 concentrations during the third growing season. Different response of the two tree species to elevated CO2 mainly resulted from the difference in the growth rate. Elevated CO2 concentrations inhibited needle dark respiration of both P. koraiensis and P. sylvestriformis during the fourth growing season. There was consistent trend be- tween the short-term effect and the long-term effect of elevated CO2 on needle dark respiration in P. sylvestriformis during the third growing season by changing measurement CO2 concentrations. How- ever, the short-term effect was different from the long-term effect for P. koraiensis. Response of dark respiration of P. koraiensis and P. sylvestriformis to elevated CO2 concentrations was related to the treatment time of CO2 and the stage of growth and development of plant. The change of dark respiration for the two tree species was determined by the direct effect of CO2 and long-term acclimation. The prediction of the long-term response of needle dark respiration to elevated CO2 concentration based on the short-term response is in dispute.

  17. Impact of Experimentally Elevated Ozone on Seed Germination and Growth of Russian Pine (Pinus sylvestris) and Spruce (Picea spp.) Provenances

    NARCIS (Netherlands)

    Prozherina, Nadezda; Nakvasina, Elena; Oksanen, Elina

    2009-01-01

    The impact of elevated ozone concentrations on early ontogenetic stages of pine (Pinus sylvestris) and spruce (Picea abies, Picea obovata, P. abies X P. obovata) seedlings originating from different provenances in Russia were studied in the open-field ozone fumigation system located in Kuopio, Finla

  18. In vitro zygotic embryo culture of Pinus peuce Gris.: Optimization of culture conditions affecting germination and early seedling growth

    Directory of Open Access Journals (Sweden)

    Stojičić Dragana

    2012-01-01

    Full Text Available This study reports a protocol for the germination and early seedling growth of Pinus peuce Gris. using zygotic embryo culture. In order to overcome seed dormancy and optimize organogenesis, the effect of nutritional, plant growth regulatory and physical factors on in vitro germination and growth of isolated mature zygotic embryos of P. peuce were investigated.

  19. [Genetic diversity of isoenzymes in mountain pine (Pinus mugo Turra) in natural populations in the Ukrainian Carpathian mountains].

    Science.gov (United States)

    Pirko, Ia V

    2000-01-01

    Electrophoretic spectra of GOT, GDH, DIA, MDH, SOD, FDH, ADH, ACP, IDH enzymes in the megagametophytes of seeds of 69 mountain pine (Pinus mugo Turra) trees from natural populations of the Ukrainian Carpathian mountains have been described. 19 loci products had efficient electrophoretic separation. The analysis of alleles segregation of the heterozygous trees on the whole confirms monogenic inheritance of the discovered variants.

  20. Enhanced drought-tolerance in the homoploid hybrid species Pinus densata: implication for its habitat divergence from two progenitors.

    Science.gov (United States)

    Ma, Fei; Zhao, Changming; Milne, Richard; Ji, Mingfei; Chen, Litong; Liu, Jianquan

    2010-01-01

    The homoploid hybrid species Pinus densata is restricted to alpine habitats that exceed the altitude range of its two parental species, Pinus tabulaeformis and Pinus yunnanensis. Alpine habitats usually generate cold-induced water stress in plants. To understand the ecological differentiation between these three species, we examined their physiological responses to drought stress. Potted seedlings of three species were subjected to low, mild, moderate and severe water stress in an automatic-controlled glasshouse. Fifteen indicators of fitness were measured for each species in each treatment, and most of these decreased as drought increased. Pinus densata exhibited higher fitness than both parental species in terms of total dry mass production (TDM) and long-term water use efficiency (WUE(L)) across all treatments; several other ecophysiological traits were also extreme but not across every treatment, and not always in the highest stress treatment. These results indicate that extreme characters that have become well fixed in P. densata, confer a faster seedling growth rate and more efficient water use, which in turn should confer increased drought tolerance. These traits of P. densata likely promoted its ecological separation from its parental species and facilitated its successful colonization and establishment in high-altitude habitats.

  1. Can we expect to protect threatened species in protected areas? A case study of the genus Pinus in Mexico

    NARCIS (Netherlands)

    Aguirre Gutiérrez, J.; Duivenvoorden, J.F.

    2010-01-01

    The distribution of 56 Pinus species in Mexico was modelled with MAXENT. The pine species were classified as threatened according to IUCN criteria. Our aim was to ascertain whether or not threatened pine species were adequately represented in protected areas. Almost 70% of the species had less than

  2. Efeito da densidade e da distância de caruru-de-mancha e amendoim-bravo na cultura do feijoeiro Effect of the density and distance of slender amaranth and milkweed on the common bean (Phaseolus vulgari

    Directory of Open Access Journals (Sweden)

    A.A.M. Barroso

    2012-03-01

    Full Text Available O objetivo deste trabalho foi avaliar a interferência causada pelo caruru-demancha (Amaranthus viridis e amendoim-bravo (Euphorbia heterophylla, em função das densidades e distâncias, no feijoeiro (Phaseolus vulgaris cultivar Pérola. Como recipientes, foram utilizadas caixas de cimento-amianto, com capacidade para 50 litros, preenchidas com LatossoloVermelho-Escuro. As mudas foram formadas em bandejas de 128 células preenchidas com substrato hortícola; quando as plântulas atingiram o estádio V2, foram transplantadas para as caixas, sendo as de feijoeiro numa linha central, reproduzindo a semeadura em campo, e as das plantas daninhas nas densidades de 8, 16 e 32 plantas m-2, distanciadas de 0, 12 e 24 cm das plantas de feijão e igualmente entre si. O experimento foi conduzido no delineamento experimental de blocos casualizados, com os tratamentos dispostos em esquema fatorial 3x3+2T, com quatro repetições, constituindo as parcelas experimentais. Foram avaliadas características de crescimento e de produtividade da cultura e das plantas daninhas. Os dados obtidos foram submetidos à análise de variância pelo teste F, e as médias, comparadas pelo teste de Tukey. Observou-se que as plantas daninhas obtiveram maior desenvolvimento quando em maior distância da cultura. O caruru-de-mancha causou reduções no número de vagens e na produtividade estimada do feijoeiro. Para o caruru-de-mancha, o aumento da densidade só causou redução na produtividade da cultura quando as plantas estavam distanciadas em pelo menos 12 cm. A 0 cm, o feijoeiro tornou-se mais competitivo e não sofreu interferência das plantas daninhas, independentemente da densidade destas.The aim of this study was to evaluate the interference caused by Slender amaranth (Amaranthus viridis and Milkweed (Euphorbia heterophylla at different densities and distances in the common bean (Phaseolus vulgaris cv. Pérola. The experiment was carried out using asbestos cement boxes

  3. 四种松属植物叶的抗寒抗旱特点研究%Study on Dry Resistance and Cold Resistance of Leaf of Pinus

    Institute of Scientific and Technical Information of China (English)

    倪福太; 李长有; 王占武; 刘强

    2012-01-01

    为了研究松属植物叶片与植物抗寒抗旱特点的关系,以松属(Pinus)两种植物樟子松(Pinus sylvestris var.mongolica Litvin.)和油松(Pinus tabulaeformis Carr.)为主要实验材料,同时,观察黑松(Pinus thunbergii Parl.)和马尾松(Pinus massoniana Lamb.),研究针叶的表皮特征及叶片不同部位的解剖结构.结果表明,松属植物叶表皮外具有发达的角质层,气孔下陷,具有大的孔下室,树脂道发达.这说明松属植物叶具有旱生和寒性植物的形态结构特征.%In order to study the relationship between leaf of Pinus and drought resistance and cold resistance,epidermis characteristic and anatomy construction of needle leaf of two specices plants(Pinus sylvestrisa var.mongolica Litvin.and Pinus tabulaeformis Carr.) in Pinus are observed.The results indicated that the leaf of Pinus plants have some construction of drought resistance and cold resistance.For example:the outside on the surface of leaf is covered with a cuticle and epicuticular waxes,its stomata are stand out of the epidermis with big bore bottom room and so on.

  4. Natural production of Tuber aestivum in central Spain: Pinus spp. versus Quercus spp. brûlés

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    Luis G. Garcia-Montero

    2014-08-01

    Full Text Available Aim of study: Tuber aestivum is the most widespread edible truffle, with increasing commercial interest. This species can produce carpophores with conifer hosts, in contrast with the inability of Pinus spp. to induce fruiting in other truffle species such as Tuber melanosporum. Therefore the objective is to compare the characteristics and carpophore production of T. aestivum brûlés associated with Pinus spp. versus Quercus spp.Area of study: We studied the natural habitats of T. aestivum in the Alto Tajo Nature Reserve in central Spain.Material and methods: During 5 years, we monitored the production of carpophores and brûlé size of 145 T. aestivum brûlés associated with Pinus nigra subsp. salzmanni and P. sylvestris and Quercus ilex subsp. ballota and Q. faginea hosts. Statistical treatment was performed using the Statistica Program v. 6.Main Results: The size of brûlés associated with Pinus was significantly smaller than that of brûlés associated with Quercus. However, carpophore production per brûlé, and especially for brûlés of similar size, was greater when the host plant was a pine. After accounting for brûlé size, the production of brûlés associated with Pinus spp. was 2.23 (95% CI, between 1.35 and 3.69 and 1.61 (95% CI, between 1.02 and 2.54 times greater than the production of brûlés associated with Quercus faginea and Q. ilex subsp. ballota, respectively.Research highlights: The considerable ability of Pinus nigra subsp. salzmanni and P. sylvestris to form effective brûlés and to produce carpophores of Tuber aestivum in natural conditions was clearly demonstrated, and suggest that those species can be of use in the culture of T. aestivum.Key words: Summer truffle; Tuber aestivum; truffle culture; truffle ecology; Pinus spp.; Quercus spp.

  5. Chromatographic fingerprint analysis of Pycnogenol® dietary supplements

    Science.gov (United States)

    French maritime bark (Pinus maritima) has been widely used as an herbal remedy for various degenerative diseases. A standardized bark extract is available that complies with its USP monograph and is derived from Pinus pinaster, Ait. (Pycnogenol®, Horphag Research Ltd., UK). The method specified in...

  6. Mir v Indonezii : Bravo, Ahtisaari! / Gennadi Muravin

    Index Scriptorium Estoniae

    Muravin, Gennadi

    2005-01-01

    Soome presidendi Martti Ahtisaari eesistumisel toimunud läbirääkimistel suutsid Indoneesia valitsus ja Acehi provintsi mässulised jõuda kokkuleppele sõjategevuse, mis Sumatra saarel on kestnud ligi 30 aastat, lõpetamiseks

  7. Bravo! China: Experience Chinese Culture in Africa

    Institute of Scientific and Technical Information of China (English)

    LiShegxian

    2004-01-01

    On july 13,2004,"Hail for China and Africa; A Chinese Cultural Tour of Africa" was launched in Prertoria,South Africa,Senior Officials from china and South Africa attended the opening ceremony,including Chinese State Councilor Madame Chen Zhili ,South Africa cultural minister,agricultural minister and mayor of Pretoria.

  8. Robin Wood, "Rio Bravo," and Me

    Science.gov (United States)

    Leith, Dick

    2011-01-01

    The film scholar Robin Wood, who died recently, taught me English during the mid-1960s at Welwyn Garden City High School (now Sir Frederick Osborn Comprehensive). He was one of those teachers who "made a difference". He helped me learn that it was all right for a boy from a working-class home to study stuff such as literature. But it was…

  9. [Physiological characteristics of Pinus densiflora var. zhangwuensis and Pinus sylvestris var. mongolica seedlings on sandy lands under salt-alkali stresses].

    Science.gov (United States)

    Meng, Peng; Li, Yu-Ling; Zhang, Bai-xi

    2013-02-01

    For the popularization of Pinus densiflora var. zhangwuensis, a new afforestation tree species on the desertified and salinized-alkalized lands in Northern China, and to evaluate the salinity-alkalinity tolerance of the tree species and to better understand the tolerance mechanisms, a pot experiment with 4-year old P. densiflora var. zhangwuensis and P. sylvestris var. mongolica was conducted to study their seedlings growth and physiological and biochemical indices under the effects of three types salt (NaCl, Na2CO3, and NaHCO3 ) stresses and of alkali (NaOH) stress. Under the salt-alkali stresses, the injury level of P. densiflora var. zhangwuensis was lower, and the root tolerance index was higher. The leaf catalase (CAT) activity increased significantly by 22. 6 times at the most, as compared with the control; the leaf malondialdehyde (MDA) content had no significant increase; the leaf chlorophyll (Chl) content had a smaller decrement; and the leaf water content (LWC) increased slightly. P. sylvestris var. mongolica responded differently to the salt-alkali stresses. Its leaf CAT activity had less change, MDA content increased significantly, Chl content had significant decrease, and LWC decreased slightly. It was suggested that P. densi-flora var. zhangwuensis had a greater salinity-alkalinity tolerance than P. sylvestris var. mongolica. The higher iron concentration in P. densiflora var. zhangwuensis needles enhanced the CAT activity and Chl content, whereas the higher concentrations of zinc and copper were associated with the stronger salinity-alkalinity tolerance.

  10. Metal(loid) allocation and nutrient retranslocation in Pinus halepensis trees growing on semiarid mine tailings

    Energy Technology Data Exchange (ETDEWEB)

    Parraga-Aguado, Isabel, E-mail: isabel.parraga@upct.es [Universidad Politecnica de Cartagena, Departamento de Ciencia y Tecnología Agraria Paseo Alfonso XIII, Cartagena 48. 30203 (Spain); Querejeta, Jose-Ignacio [Water and Soil Conservation Department, Centro de Edafología y Biología Aplicada del Segura CEBAS-CSIC Campus Universitario de Espinardo, PO Box 164, Espinardo-Murcia ES-30100 (Spain); González-Alcaraz, María Nazaret; Conesa, Hector M. [Universidad Politecnica de Cartagena, Departamento de Ciencia y Tecnología Agraria Paseo Alfonso XIII, Cartagena 48. 30203 (Spain)

    2014-07-01

    The goal of this study was to evaluate internal metal(loid) cycling and the risk of metal(loid) accumulation in litter from Pinus halepensis trees growing at a mine tailing disposal site in semiarid Southeast Spain. Internal nutrient retranslocation was also evaluated in order to gain insight into the ability of pine trees to cope with the low-fertility soil conditions at the tailings. We measured metal(loid) concentrations in the foliage (young and old needles), woody stems and fresh leaf litter of pine trees growing on tailings. The nutrient status and stable isotope composition of pine foliage (δ{sup 13}C, δ{sup 15}N, δ{sup 18}O as indicators of photosynthesis and water use efficiency) were also analyzed. Tailing soil properties in vegetation patches and in adjacent bare soil patches were characterized as well. Significant amounts of metal(loid)s such us Cd, Cu, Pb and Sb were immobilized in the woody stems of Pinus halepensis trees growing on tailings. Leaf litterfall showed high concentrations of As, Cd, Sb, Pb and Zn, which thereby return to the soil. However, water extractable metal(loid) concentrations in tailing soils were similar between vegetation patches (mineral soil under the litter layer) and bare soil patches. The pines growing on mine tailings showed very low foliar P concentrations in all leaf age classes, which suggests severe P deficiency. Young (current year) needles showed lower accumulation of metal(loid)s, higher nutrient concentrations (P and K), and higher water use efficiency (as indicated by and δ{sup 13}C and δ{sup 18}O data) than older needles. Substantial nutrient resorption occurred before leaf litterfall, with 46% retranslocation efficiency for P and 89% for K. In conclusion, phytostabilization of semiarid mine tailings with Pinus halepensis is feasible but would require careful monitoring of the trace elements released from litterfall, in order to assess the long term risk of metal(loid) transfer to the food chain

  11. Phytosociology, structure and dynamics of Pinus roxburghii associations from Northern Pakistan

    Institute of Scientific and Technical Information of China (English)

    Nasrullah Khan; Kishwar Ali; Shahid Shaukat

    2014-01-01

    We investigated the phytosociology, structure and dynamics of Pinus roxburghii in 40 stands in northern areas of Pakistan by using cluster analysis (Ward’s agglomerative clustering) and ordination (Non-metric Multidimensional Scaling). Cluster analysis revealed three major groups associated with specific environmental characteristics: (1) P. roxburghii (2) Pinus-Quercus baloot and (3) Pinus-Olea ferruginea community types. NMS-ordination showed the major gradient as an amalgam of elevation (r2=0.441, p<0.01) and slope (r2=0.391, p<0.05) as the two topographic factors correlated with species distribution. The first ordination axis also showed positive correlation with soil variables like pH and electrical conductivity, suggesting that soil chemistry was related to topographic characteristics and probably acted as a secondary gradient. We also examined size class distributions, age structures and growth rates of the three communities in order to describe community development and dynamics. Total tree density was 14700 plants/ha, with P. roxburghii having a relative density of 82%to 100%. Density of juve-nile and total density and basal area of the subordinate tree species were low. The low density of trees in the smallest diameter size-class sug-gested that the recruitment of small P. roxburghii plants into the adult population may be lower than the required replacement rate for the stands. Pooled size-class distributions for the species showed a multimo-dal pattern with some regeneration gaps. Browsing, heavy logging and other anthropogenic activities were the overriding factors responsible for the poor recruitment of P. roxburghii. We concluded from the age struc-ture that the forests were characterized by the dominance of young trees. Growth rate analysis revealed that P. roxburghii was the fastest growing species among the conifers species in Pakistan. In view of its relatively fast growth and longevity, P. roxburghii seems to be a suitable choice for short

  12. [Effects of elevated ozone on Pinus armandii growth: a simulation study with open-top chamber].

    Science.gov (United States)

    Liu, Chang-Fu; Liu, Chen; He, Xing-Yuan; Ruan, Ya-Nan; Xu, Sheng; Chen, Zhen-Ju; Peng, Jun-Jie; Li, Teng

    2013-10-01

    By using open-top chamber (OTC) and the techniques of dendrochronology, this paper studied the growth of Pinus armandii under elevated ozone, and explored the evolution dynamics and adaptation mechanisms of typical forest ecosystems to ozone enrichment. Elevated ozone inhibited the stem growth of P. armandii significantly, with the annual growth of the stem length and diameter reduced by 35.0% and 12.9%, respectively. The annual growth of tree-ring width and the annual ring cells number decreased by 11.5% and 54.1%, respectively, but no significant change was observed in the diameter of tracheid. At regional scale, the fluctuation of ozone concentration showed significant correlation with the variation of local vegetation growth (NDVI).

  13. Development and Characterization of 25 EST-SSR markers in Pinus sylvestris var. mongolica (Pinaceae

    Directory of Open Access Journals (Sweden)

    Pan Fang

    2014-01-01

    Full Text Available Premise of the study: A set of novel expressed sequence tag (EST microsatellite markers was developed in Pinus sylvestris var. mongolica to promote further genetic studies in this species. Methods and Results: One hundred seventy-five EST–simple sequence repeat (SSR primers were designed and synthesized for 31,653 isotigs based on P. tabuliformis EST sequences. The primer pairs were used to identify 25 polymorphic loci in 48 individuals. The number of alleles ranged from two to eight with observed and expected heterozygosity values of 0.0435 to 0.8125 and 0.0430 to 0.7820, respectively. Conclusions: These new polymorphic EST-SSR markers will be useful for assessing genetic diversity, molecular breeding and genetic improvement, and conservation of P. sylvestris var. mongolica.

  14. Soil Type Affects Pinus ponderosa var. scopulorum (Pinaceae Seedling Growth in Simulated Drought Experiments

    Directory of Open Access Journals (Sweden)

    Alexander J. Lindsey

    2013-07-01

    Full Text Available Premise of the study: Effects of drought stress and media type interactions on growth of Pinus ponderosa var. scopulorum germinants were investigated. Methods and Results: Soil properties and growth responses under drought were compared across four growth media types: two native soils (dolomitic limestone and granite, a soil-less industry standard conifer medium, and a custom-mixed conifer medium. After 35 d of growth, the seedlings under drought stress (reduced watering produced less shoot and root biomass than watered control seedlings. Organic media led to decreased root biomass, but increased root length and shoot biomass relative to the mineral soils. Conclusions: Media type affected root-to-shoot biomass partitioning of P. ponderosa var. scopulorum, which may influence net photosynthetic rates, growth, and long-term seedling survival. Further work should examine how specific soil properties like bulk density and organic matter influence biomass allocation in greenhouse studies.

  15. Antimicrobial terpenes from oleoresin of ponderosa pine tree Pinus ponderosa: A defense mechanism against microbial invasion

    Energy Technology Data Exchange (ETDEWEB)

    Himejima, Masaki; Hobson, K.R.; Otsuka, Toshikazu; Wood, D.L.; Kubo, Isao (Univ. of California, Berkeley (United States))

    1992-10-01

    The oleoresin of the ponderosa pine, Pinus ponderosa (Pinaceae) exhibited broad antimicrobial activity. In order to identify the active compounds, the oleoresin was steam distilled to give a distillate and residue. The distillate contained mainly monoterpenes and some sesquiterpenes, while the residue consisted chiefly of four structurally related diterpene acids. An antimicrobial assay with the pure compounds indicated that the monoterpenes were active primarily against fungi, but there was also some activity against gram-positive bacteria. The diterpene acids, in contrast, only exhibited activity against gram-positive bacteria. Although not all of the identified sesquiterpenes could be tested, longifolene showed activity only against gram-positive bacteria. Therefore, it appears that the oleoresin of P. ponderosa functions as a biochemical defense against microbial invasion.

  16. Genetic Differentiation of Pinus koraiensis under Different Altitude Conditions in Changbai Mountain

    Institute of Scientific and Technical Information of China (English)

    FENGFujuan

    2004-01-01

    The genetic differentiation of Korean pine (Pinus koraiensis) in different altitudes in Changbai Mountain was analyzed by ISSR technique, and it was found that the level of genetic diversity of Korean pine reduces along with altitude increasing in Changbai Mountain. The variation of Korean pine is mainly from intra-population and there is a positive relativity between genetic distance and vertical geographic distance of Korean pine in different altitudes. The genetic coherence shows that altitude has less insulation to Korean pine. Therefore, it is deduced that the terrain formation of vertical distribution of Korean pine is a result of diffusion from lower altitude to higher altitude in the course of enlarging its adaptability.

  17. Ectomycorrhizae of Tuber huidongense and T. liyuanum with Castanea mollissima and Pinus armandii.

    Science.gov (United States)

    Wan, Shan-Ping; Yu, Fu-Qiang; Tang, Li; Wang, Ran; Wang, Yun; Liu, Pei-Gui; Wang, Xiang-Hua; Zheng, Yi

    2016-04-01

    Tuber huidongense and T. liyuanum are common commercial white truffles in China that belong to the Rufum and Puberulum groups of the genus Tuber, respectively. Their mycorrhizae were successfully synthesized with two native trees--Castanea mollissima and Pinus armandii--under greenhouse conditions. The identities of the mycorrhizae were confirmed through internal transcribed spacer (ITS) sequence analyses, and their morphological characteristics were described. All of the obtained mycorrhizae have an interlocking pseudoparenchymatous mantle, which is a typical feature of truffle mycorrhizae. The mycorrhizae of T. huidongense on the two trees have hyaline branched emanating hyphae, similar to the documented mycorrhizae of the Rufum group. The unramified, spiky, and hyaline cystidia on the mycorrhizae of T. liyuanum with both C. mollissima and P. armandii further confirmed that this characteristic is constant for the mycorrhizae of the Puberulum group. The successful mycorrhizal syntheses on the two nut-producing trees will be of economic importance in the cultivation of the two truffles.

  18. Crystallization and initial crystallographic characterization of a vicilin-type seed storage protein from Pinus koraiensis.

    Science.gov (United States)

    Jin, Tengchuan; Fu, Tong Jen; Kothary, Mahendra H; Howard, Andrew; Zhang, Yu Zhu

    2007-12-01

    The cupin superfamily of proteins includes the 7S and 11S seed storage proteins. Many members of this family of proteins are known allergens. In this study, the Korean pine (Pinus koraiensis) vicilin-type 7S seed storage protein was isolated from defatted pine-nut extract and purified by sequential gel-filtration and anion-exchange chromatography. Well diffracting single crystals were obtained by the vapor-diffusion method in hanging drops. The crystals belong to the primitive cubic space group P2(1)3, with unit-cell parameters a = b = c = 148.174 A. Two vicilin molecules were present in the asymmetric unit and the Matthews coefficient was determined to be 2.90 A(3) Da(-1), with a corresponding solvent content of approximately 58%. A molecular-replacement structural solution has been obtained using the program Phaser. Refinement of the structure is currently under way.

  19. Analysis of inter - population variability Scots pine (Pinus sylvestris L. using morphometric markers

    Directory of Open Access Journals (Sweden)

    Lučić Aleksandar

    2012-01-01

    Full Text Available The aim of this study is analysis of inter-population variability of Scots pine (Pinus sylvestris L. using morphometric parameters of cones and seedlings originating from seven populations in Serbia.The analysis of 1960 cones and their seeds was performed as well as the analysis of morphometric parameters of seedlings (height and root neck diameter that were produced from this seed. Based on the obtained results the significant differences were noticed in the values of analyzed characters for each population separately. The biological similarity or distance regarding the analyzed characters showed a significant population differentiation. By presenting the preliminary knowledge on the genetic variability of the study populations, the reported results will contribute to better understanding of the significance of the analyzed characters in the population differentiation. [Projekat Ministarstva nauke Republike Srbije, br. TR 43007

  20. [Effects of Pinus armandii seed size on rodents caching behavior and it's spatio-temporal variations].

    Science.gov (United States)

    Chen, Fan; Chen, Jin

    2011-08-01

    Pinus armandii, a native pine species, has large (about 300 mg), wingless seeds, and distributes from central to western China at an altitude of 1000-3300 m. To determine how the seed size affects rodents caching behavior, tagged seed releasing and tracking experiments were conducted at 3 sites in Northwest Yunnan province in 2006 and 2007. Our data indicated that for all sites and both years, compared with the smaller seeds, the proportions of cached large seeds were significantly higher, whereas the consumed ones were significantly lower. Meanwhile, the mean and maximum values of caching distances were also significantly increased in large seeds. Seed fate was different between the two years and within the three sites as there have different rodent community compositions.

  1. Factors influencing germination and growth of isolated embryos of Pinus heldreichii

    Directory of Open Access Journals (Sweden)

    Stojičić Dragana

    2008-01-01

    Full Text Available The effects of nutritional, hormonal, and physical factors on the germination and growth of isolated mature zygotic embryos of Pinus heldreichii were studied under in vitro conditions. The optimum medium for embryo germination and adequate conversion into seedlings was basal Gresshoff and Doy (1GD medium. All tested carbohydrates at the applied concentrations stimulated embryo growth, but only 3% sucrose increased embryo germination compared to the control, while maltose had an inhibitory effect. Among the applied plant growth regulators, only gibberelic acid (GA3 had a few benefits on the development of isolated embryos into plantlets, while the others had a negative effect. Embryos cultured on an agar-solidified medium grew better and the germination percentage was higher than in a liquid medium. Light treatment did not affect embryo germination, but generally stimulated seedling growth.

  2. MODELLING GROWTH AND YIELD OF Pinus taeda L. USING DIFUSION PROCESS

    Directory of Open Access Journals (Sweden)

    Rozane de Loyola Eisfeld

    2005-06-01

    Full Text Available This work tested a methodology for growth and yield modeling. The diffusion process is not yet widely used incommercial plantations in Brazil, but it can provide predictions comparable to others methodologies, producing satisfactory resultsto simulate growth and yield. For this purpose, 325 permanent samples established in unthinned Pinus taeda L. (loblolly pine standsowned by the International Paper of Brazil Co were used. The diffusion process methodology consists in connecting growthincrement and mortality models in Kolmogorov equation . Seventy sample plots were randomly chosen in order to make thecomparison among the observed and predicted values. In general, the diffusion process provided satisfactory estimates of number oftrees, basal area per hectare and stem volume.

  3. Identification and characterization of microsatellite markers in Pinus kesiya var. langbianensis (Pinaceae)1

    Science.gov (United States)

    Cai, Nian-Hui; Xu, Yu-Lan; Wang, Da-Wei; Chen, Shi; Li, Gen-Qian

    2017-01-01

    Premise of the study: Microsatellite primers were developed in Pinus kesiya var. langbianensis (Pinaceae), a species native to southwestern China, to investigate its genetic diversity and population structure in order to provide information for the conservation and management of this species. Methods and Results: Using next-generation sequencing, a total of 2349 putative simple sequence repeat primer pairs were designed. Eighteen polymorphic markers in 60 individuals belonging to four populations of P. kesiya var. langbianensis were identified and characterized with two to 11 alleles per locus. The observed and expected heterozygosity ranged from 0.000 to 0.800 and 0.000 to 0.840, respectively. Each of these loci cross-amplified in the closely related species P. massoniana, P. densata, P. tabuliformis, and P. yunnanensis, with one to seven alleles per locus. Conclusions: The new markers are promising tools to study the population genetics of P. kesiya var. langbianensis and related species. PMID:28224057

  4. Quantification and characterization of Si in Pinus Insignis Dougl by TXRF

    Energy Technology Data Exchange (ETDEWEB)

    Navarro, Henry; Bennun, Leonardo [Universidad de Concepcion, Laboratorio de Fisica Aplicada, Departamento de Fisica, Concepcion (Chile); Marco, Lue M. [Universidad Centro Lisandro Alvarado, Decanato de Agronomia, Depto. de Quimica, Barquisimeto (Venezuela, Bolivarian Republic of)

    2014-12-09

    A simple quantification of silicon is described, in woods such as Pinus Insigne Dougl obtained from the 8th region of Bio-Bio, 37 15'' South-73 19'' West, Chile. The samples were prepared through fractional calcination, and the ashes were directly analyzed by total reflection X-ray fluorescence (TXRF) technique. The analysis of 16 samples that were calcined is presented. The samples were weighed on plastic reflectors in a microbalance with sensitivity of 0.1 μg. Later, the samples were irradiated in a TXRF PICOFOX spectrometer, for 350 and 700 s. To each sample, cobalt was added as an internal standard. Concentrations of silicon over the 1 % in each sample and the self-absorption effect on the quantification were observed, in masses higher than 100 μg. (orig.)

  5. Rooting of Mugo pine (Pinus mugo cuttings as affected by IBA, NAA and planting substrate

    Directory of Open Access Journals (Sweden)

    Shahram Sedaghathoor

    2016-07-01

    Full Text Available Aim of study: The effect of planting substrate and concentrations of indole-3-butyric acid (IBA and naphthaleneacetic acid (NAA hormones was studied on the rooting of mugo pine cuttings. Area of study: The research was carried out in Rasht city, Guilan province, Iran. Material and Methods: Both hormones (IBA and NAA were applied at four concentrations of 0, 1000, 2000 and 4000 mg/l. Planting substrates included sand, perlite, cocopeat, sand + perlite, and sand + cocopeat (1:1. Main results: The highest rooting percentage (55% was obtained under the trilateral treatment a2b4c1 (sand × 4000 mg/l NAA × 1000 mg/l IBA. Sand + cocopeat was found to be the best rooting substrate. Research highlights: It is recommended to apply sand with 4000 mg/l and 1000mg/l concentration of experimental hormones (NAA and IBA, respectively. Keywords: auxin; rooting; Pinus mugo; vegetative propagation.

  6. Inheritance and subcellular localization of triose-phosphate isomerase in dwarf mountain pine (Pinus mugo).

    Science.gov (United States)

    Odrzykoski, I J

    2001-01-01

    Several trees with expected heterozygous phenotype for triose-phosphate isomerase (TPI) were discovered in a population of dwarf mountain pine (Pinus mugo Turra) from southern Poland. As the inheritance of this enzyme in pines has not been reported, segregation of allelic variants was tested in eight trees with putative heterozygous phenotypes for two loci, TpiA and TPIB: Linkage between these and some other isozyme loci were studied and evidence for linkage has been found between TpiA and PgdA (r = 0.10) and between TpiB and DiaD (r = 0.36), but in single trees only. The subcellular localization of TPI isozymes was determined by comparing isoenzymes from the total extract with those found in fraction enriched in plastids, prepared by differential gradient centrifugation of cellular organelles. The more slowly migrating TPI-B isozyme is located in plastids.

  7. Morphological differences between Pinus mugo Turra populations from the Tatra Mts. revealed by cone traits

    Directory of Open Access Journals (Sweden)

    Maria A. Bobowicz

    2014-01-01

    Full Text Available Two-year-old cones were collected from 388 dwarf mountain pine (Pinus mugo Turra plants of ten populations of this species from the Tatra Mts. - five of them from calcareous and five from calcium-free undersoil. Their 14 morphological traits are described. The data served for performing multivariate analysis of variance and testing of statistical hypotheses, for discriminate analysis. for calculating Mahalanobis distances between populations and plotting a dendrite based on the shortest Mahalanobis distances and for agglomerative clustering by the method of nearest neighbourhood. Wide differences in the populations were found as regards all the studied traits and the existence of two groups in the population, which, do not, however, correlate with the substrate type. The "calcareous and calcium-free" populations show statistically significant differences in six of the 14 studied traits.

  8. Distribution of elements in needles of Pinus massoniana (Lamb.) was uneven and affected by needle age.

    Science.gov (United States)

    Kuang, Yuan Wen; Wen, Da Zhi; Zhou, Guoyi; Liu, Shi Zhong

    2007-02-01

    Macronutrients (P, S, K, Na, Mg, Ca), heavy metals (Fe, Zn, Mn, Cu, Pb, Cr, Ni, Cd,) and Al concentrations as well as values of Ca/Al in the tip, middle and base sections, and sheaths of current year and previous year needles of Pinus massoniana from Xiqiao Mountain were analyzed and the distribution patterns of those elements were compared. The results indicated that many elements were unevenly distributed among the different components of needles. Possible deficiency of P, K, Ca, Mn and Al toxicity occurred in needles under air pollution. Heavy metals may threaten the health of Masson pine. Needle sheaths were good places to look for particulate pollutants, in this case including Fe, Cu, Zn, Pb, Cr, Cd and Al.

  9. Assessment of holocellulose for the production of bioethanol by conserving Pinus radiata cones as renewable feedstock.

    Science.gov (United States)

    Victor, Amudhavalli; Pulidindi, Indra Neel; Gedanken, Aharon

    2015-10-01

    Renewable and green energy sources are much sought. Bioethanol is an environmentally friendly transportation fuel. Pine cones from Pinus radiata were shown to be a potential feedstock for the production of bioethanol. Alkaline (NaOH) pretreatment was carried out to delignify the lignocellulosic material and generate holocellulose (72 wt. % yield). The pretreated biomass was hydrolysed using HCl as catalyst under microwave irradiation and hydrothermal conditions. Microwave irradiation was found to be better than the hydrothermal process. Microwave irradiation accelerated the hydrolysis of biomass (42 wt. % conversion) with the reaction conditions being 3 M HCl and 5 min of irradiation time. Interestingly, even the xylose, which is the major component of the hydrolyzate was found to be metabolized to ethanol using Baker's yeast (Saccharomyces cerevisiae) under the experimental conditions. 5.7 g of ethanol could be produced from 100 g of raw pine cones.

  10. Wood properties of Eucalyptus grandis and Pinus caribaea estimated by colorimetry

    Directory of Open Access Journals (Sweden)

    Patrícia Gomes Ribeiro Amorim

    2013-09-01

    Full Text Available The use of non-destructive technologies has been very effective in characterization and evaluation of wood quality. This study aimed to characterize the technologically wood of Pinus caribaea and Eucalyptus grandis by Colorimetry. The study was conducted at the University of Brasilia (UNB in Physics and Engineering Laboratory the Laboratory of Forest Products/LPF - Brazilian Forest Service (SFB. Species were subjected to standard tests of basic density, shrinkage and bending. For the colorimetric determination of the parameters it was used a spectrophotometer according to the methodology adopted by Camargos (1999. The radial surface of the wood is most suitable for the collecting of the colorimetric parameters and prediction of properties. Property best estimated by this technique was the density, followed by modulus of elasticity (MOE and modulus of rupture (MOR.

  11. [Genetic structure of the populations of Pallas pine (Pinus pallasiana D. Don) reforested in extreme conditions].

    Science.gov (United States)

    Korshikov, I I; Krasnoshtan, O V

    2010-01-01

    The paper reports on an intensive reforestation of Pallas pine (Pinus pallasiana D. Don) in post-fire sites in the native Mountainous Crimean populations and around the previously planted seed-producing trees in ore-mining dumps of the Krivoy Rog region. Self-sown progeny growing in the dump is characterized by a better growth and comes to the reproductive development phase earlier compared to that one growing in post-fire forest. Allele variability at 20 allozyme loci is less in self-sown progeny than in the native populations whereas its heterozygosity level is similar. Genetic distance (D(N)) among self-sown progenies in post-fire sites and ore-mining dump is comparable to that of the natural populations.

  12. The effects of heat treatment on some technological properties of Scots pine (Pinus sylvestris L.) wood.

    Science.gov (United States)

    Korkut, Süleyman; Akgül, Mehmet; Dündar, Turker

    2008-04-01

    Heat treatment is often applied to wood species to improve their dimensional stability. This study examined the effect of heat treatment on certain mechanical properties of Scots pine (Pinus sylvestris L.), which has industrially high usage potential and large plantations in Turkey. Wood specimens obtained from Bolu, Turkey, were subjected to heat treatment under atmospheric pressure at varying temperatures (120, 150 and 180 degrees C) for varying durations (2, 6 and 10h). The test results of heat-treated Scots pine and control samples showed that technological properties including compression strength, bending strength, modulus of elasticity in bending, janka-hardness, impact bending strength and tension strength perpendicular to grain suffered with heat treatment, and increase in temperature and duration further diminished technological strength values of the wood specimens.

  13. Effect of the heat treatment on physical and chemical properties of Pinus caribaea wood

    Directory of Open Access Journals (Sweden)

    Dallyene da Silva Poubel

    2013-09-01

    Full Text Available This study evaluated the effect of the heat treatment on physical properties (density, equilibrium moisture content, mass loss, volumetric and linear swelling and shrinkage and chemical composition of normal (opposite side and compression wood of Pinus caribaea Morelet. Samples of 25 x 25 x 50 mm were heat-treated under two different temperatures: 200 and 220 ºC. The results indicated a reduction in equilibrium moisture content and swelling and shrinkage following heat treatment, independent of wood type. The compression wood showed higher longitudinal swelling than normal one, however, after heat treatment, the improvements were similar between both wood types. Samples heat-treated at 200 and 220 ºC had mass losses of 9.94 and 21.03 %, respectively. The heat treatment promoted a relative increase in lignin content and a decrease in holocellulose and α-cellulose contents, indicating the occurrence of thermal degradation of these components.

  14. Pinus strobus: past and future in Europe. A page of silvicultural history and international scientific cooperation

    Directory of Open Access Journals (Sweden)

    Stelian Radu

    2013-12-01

    Full Text Available The author presents a short overview concerning eastern white pine (Pinus strobus L. introduction in different European countries, including the period of early extension in forest culture until the first blister rust crisis occurred. The species rehabilitation at the European level and the new extension between 1927 and 1972 years period in different countries, particularly in Romania, was presented. Most of the data incorporated here were taken over from the monograph of the senior author's of this paper. However, parts of the newly acquired results in our country were taken in to account, as well. Finally, the fruitful international scientific cooperation, in favor of P. strobus, including that with I.U.F.R.O was highlighted

  15. Pinus strobus: past and future in Europe. A page of silvicultural history and international scientific cooperation

    Directory of Open Access Journals (Sweden)

    Stelian Radu

    2008-09-01

    Full Text Available The author presents a short overview concerning eastern white pine(Pinus strobus L. introduction in different European countries, including the period of early extension in forest culture until the first blister rust crisis occurred. The species rehabilitation at the European level and the new extension between 1927 and 1972 years period in different countries, particularly in Romania, was presented.Most of the data incorporated here were taken over from the monograph of the senior author's of this paper. However, parts of the newly acquired results in our country were taken in to account, as well. Finally, the fruitful international scientific cooperation, in favor of P. strobus, including that with I.U.F.R.O was highlighted.

  16. Distribution of elements in needles of Pinus massoniana (Lamb.) was uneven and affected by needle age

    Energy Technology Data Exchange (ETDEWEB)

    Kuang Yuanwen [South China Botanical Garden, Chinese Academy of Sciences, 510650 Guangzhou (China)]. E-mail: kuangyw@scbg.ac.cn; Wen Dazhi [South China Botanical Garden, Chinese Academy of Sciences, 510650 Guangzhou (China)]. E-mail: dzwen@scbg.ac.cn; Zhou Guoyi [South China Botanical Garden, Chinese Academy of Sciences, 510650 Guangzhou (China)]. E-mail: gyzhou@scbg.ac.cn; Liu Shizhong [South China Botanical Garden, Chinese Academy of Sciences, 510650 Guangzhou (China)]. E-mail: lsz@scbg.ac.cn

    2007-02-15

    Macronutrients (P, S, K, Na, Mg, Ca), heavy metals (Fe, Zn, Mn, Cu, Pb, Cr, Ni, Cd,) and Al concentrations as well as values of Ca/Al in the tip, middle and base sections, and sheaths of current year and previous year needles of Pinus massoniana from Xiqiao Mountain were analyzed and the distribution patterns of those elements were compared. The results indicated that many elements were unevenly distributed among the different components of needles. Possible deficiency of P, K, Ca, Mn and Al toxicity occurred in needles under air pollution. Heavy metals may threaten the health of Masson pine. Needle sheaths were good places to look for particulate pollutants, in this case including Fe, Cu, Zn, Pb, Cr, Cd and Al. - Pine needle sections as bioindicator for heavy metals and nutrient deficiency particularly needle sheath for particle pollutants.

  17. Distribution of elements in needles of Pinus massoniana (Lamb.) was uneven and affected by needle age

    Energy Technology Data Exchange (ETDEWEB)

    Kuang Yuanwen [Institute of Ecology, South China Botanical Garden, Chinese Academy of Sciences, 510650 Guangzhou (China)]. E-mail: kuangyw@scbg.ac.cn; Wen Dazhi [Institute of Ecology, South China Botanical Garden, Chinese Academy of Sciences, 510650 Guangzhou (China)]. E-mail: dzwen@scbg.ac.cn; Zhou Guoyi [Institute of Ecology, South China Botanical Garden, Chinese Academy of Sciences, 510650 Guangzhou (China)]. E-mail: gyzhou@scbg.ac.cn; Liu Shizhong [Institute of Ecology, South China Botanical Garden, Chinese Academy of Sciences, 510650 Guangzhou (China)]. E-mail: lsz@scbg.ac.cn

    2007-01-15

    Macronutrients (P, S, K, Na, Mg, Ca), heavy metals (Fe, Zn, Mn, Cu, Pb, Cr, Ni, Cd) and Al concentrations as well as values of Ca/Al in the tip, middle, base sections and sheaths of current year and previous year needles of Pinus massoniana from Xiqiao Mountain were analyzed and the distribution patterns of those elements were compared. The results indicated that many elements were unevenly distributed among the different components of needles. Possible deficiency of P, K, Ca, Mn and Al toxicity occurred in needles under air pollution. Heavy metals may threaten the health of Masson pine. Needle sheaths were good places to look for particulate pollutants, in this case including Fe, Cu, Zn, Pb, Cr, Cd and Al. - Pine needle sections as bioindicator for heavy metals and nutrient deficiency particularly needle sheath for particle pollutants.

  18. Relationships between foliar phosphorus fractions of Pinus sylvestris var. mongolica and soil available phosphorus

    Institute of Scientific and Technical Information of China (English)

    Qiong ZHAO; Xingyu LIU; Dehui ZENG; Jinhuan LIU; Yalin HU

    2009-01-01

    In order to find out the best foliar diagnostic index of phosphorus (P) nutrition in Mongolian pine (Pinus sylvestris var. mongolica) in the southeastern Keerqin Sandy Lands, the concentrations of total nitrogen (N), inorganic P, organic P and total P in needles of different ages and soil available P were examined. The results show that in the study area, soil available P was rather low (0.12-0.63mg/kg) and was significantly correlated with inorganic P (cPi) and total P (cPt) concentrations in current year needles of Mongolian pine. The significant correlation between soil available P and needle cPt derived from the significant correlation between cPi and cPt. Compared with cPt, cPi did reflect the level of soil P supply more accurately and more directly.

  19. Effects of Ectomycorrhizal Fungi on Growth of Seedlings of Pinus densiflora.

    Science.gov (United States)

    Sim, Mi-Yeong; Eom, Ahn-Heum

    2006-12-01

    This study was conducted to investigate the different effects of ectomycorrhizal fungal (ECMF) species on the growth of seedlings of Pinus densiflora, and the effects of ECMF diversity on plant productivity. A total of five species of ECMF were isolated from root tips of pine seedlings collected from Mt. Songni and used as inocula. Pots containing pine seedlings were inoculated with either a single ECMF species or a mixture of five ECMF species. All of the seedlings formed ECM on their roots except for the control plants. The pine seedlings' growth responses varied by the different ECMF species. Also, pine seedlings inoculated with a mixture of five ECMF species showed the highest growth response. The results of the study suggest that the colonization of diverse species of ECMF will increase plant productivity, and the selection of suitable ECMF species could be an important factor for plant growth.

  20. A new abietic acid-type diterpene glucoside from the needles of Pinus densiflora.

    Science.gov (United States)

    Jung, Mee Jung; Jung, Hyun Ah; Kang, Sam Sik; Hwang, Geum-Sook; Choi, Jae Sue

    2009-12-01

    From the ethyl acetate fraction of the methanol extract of the needles of Pinus densiflora (Pinaceae), a new diterpenoid glucoside [9alpha,13alpha-epoxy-8beta,14beta-dihydroxy-abietic acid-18-O-beta-D: -glucopyranoside] (1), two flavonoid glucosides [kaempferol 3-O-beta-D: -glucoside (2) and 6-C-methyl kaempferol 3-O-beta-D: -glucoside (3)], and two monoterpenoid glucosides [bornyl 6-O-alpha-Larabinofuranosyl (1-->6)-beta-D: -glucopyranoside (4) and bornyl 6-O-beta-D: -apiofuranosyl (1-->6)-beta-D: -glucopyranoside (5)] were isolated and characterized on the basis of spectral analysis. Of all the compounds, 2 and 3 showed peroxynitrite scavenging activity.

  1. Growth response of Pinus densiflora seedlings inoculated with three indigenous ectomycorrhizal fungi in combination

    Directory of Open Access Journals (Sweden)

    M Dalong

    2011-09-01

    Full Text Available Pinus densiflora seedlings were inoculated with three indigenous ectomycorrhizal fungi (Cenococcum geophilum, Rhizopogon roseolus and Russula densifolia in single-, two-, and three-species treatments. After 8 months, the colonization rates of each ectomycorrhizal species, seedling growth and the nutrition were assessed in each treatment. P. densiflora seedlings inoculated with different ECM species composition showed an increase in height and basal diameter and improved seedling root and shoot nutrition concentrations compared to control treatment. Generally, combined inoculation had a more positive influence on the seedlings than the single inoculation. The three-species inoculation presented the highest growth and basal diameter and concentration of most nutrients except potassium. In conclusion, the results provided strong evidence for benefits of combined inoculation with the indigenous ectomycorrhizal fungi on P. densiflora seedlings under controlled conditions.

  2. Antioxidant principles from the needles of red pine, Pinus densi fl ora.

    Science.gov (United States)

    Jung, Mee Jung; Chung, Hae Young; Choi, Jin Ho; Choi, Jae Sue

    2003-11-01

    Antioxidant activity of Pinus densiflora Sieb. et Zucc. (Pinaceae) was evaluated for potential to inhibit hydroxyl radicals, inhibit total reactive oxygen species generation in kidney homogenates using 2',7'-dichlorodihydro fluorescein diacetate (DCHF-DA) and scavenge authentic peroxynitrites. The methanolic extract of P. densiflora showed strong antioxidant activity in the tested model systems and thus fractionated with several solvents. The antioxidant activity potential of the individual fraction was in the order of ethyl acetate > n-butanol > water > dichloromethane fraction. The ethyl acetate soluble fraction exhibiting strong antioxidant activity was further puri fi ed by repeated silica gel and Sephadex LH-20 column chromatographies. An active lignan (+)-isolarisiresinol xylopyranoside, as well as two active flavonoids [kaempferol 3-O-beta-galactopyranoside and its 6"-acetyl derivative], were isolated.

  3. The mycorrhizal fungus Tricholoma matsutake stimulates Pinus densiflora seedling growth in vitro.

    Science.gov (United States)

    Guerin-Laguette, Alexis; Shindo, Katsumi; Matsushita, Norihisa; Suzuki, Kazuo; Lapeyrie, Frédéric

    2004-12-01

    While it has been suggested that Matsutake mycorrhizae might not be functional and that Matsutake may behave as a saprobic fungus in soil or even have some pathogenic activity on seedlings, we investigated the consequences of Matsutake inoculation on Pinus densiflora growth. Seventy-five days after inoculation, hyphae were anchored on short roots and well-developed Hartig net palmettis were observed. Compared to both control treatments--seedlings treated with distilled water and seedlings treated with autoclaved mycelium--inoculation significantly stimulated seedling total dry weight by 70.9% and 98.0%, respectively. These findings attest that some type of symbiotic relationship must be functional and favour host growth, ruling out claims of pathogenicity under the sterile conditions used here.

  4. Seasonal variations of monoterpene emissions from Pinus densiflora in East Asia.

    Science.gov (United States)

    Lim, Jun-Ho; Kim, Jo-Chun; Kim, Ki-Joon; Son, Youn-Suk; Sunwoo, Young; Han, Jin-Seok

    2008-09-01

    The emission rates and compositions of monoterpene from Pinus densiflora were investigated in the Gumsung (GM) and Worak (WM) mountains. The standard emission rates (ERs: ERs is the monoterpene emission rate at standard temperature, 30 degrees C) from P. densiflora ranged from 0.817 to 1.704 (microgC/gdw-h). The ERs and beta-values of total monoterpene were measured at the two study sites (GM and WM). In the spring and summer, the ERs were the highest, while relatively low values (densiflora were alpha-pinene, myrcene, beta-phellandrene, d-limonene and alpha-terpinene. The fractional compositions of individual monoterpene compounds were significantly different between the two test sites in the summer and winter. The ERs of the older group (31-40 years) were higher than those in the younger group (21-30 years). However, the monoterpene compositions were similar between the two age groups.

  5. Growth response of Pinus densiflora seedlings inoculated with three indigenous ectomycorrhizal fungi in combination.

    Science.gov (United States)

    Dalong, M; Luhe, W; Guoting, Y; Liqiang, M; Chun, L

    2011-07-01

    Pinus densiflora seedlings were inoculated with three indigenous ectomycorrhizal fungi (Cenococcum geophilum, Rhizopogon roseolus and Russula densifolia) in single-, two-, and three-species treatments. After 8 months, the colonization rates of each ectomycorrhizal species, seedling growth and the nutrition were assessed in each treatment. P. densiflora seedlings inoculated with different ECM species composition showed an increase in height and basal diameter and improved seedling root and shoot nutrition concentrations compared to control treatment. Generally, combined inoculation had a more positive influence on the seedlings than the single inoculation. The three-species inoculation presented the highest growth and basal diameter and concentration of most nutrients except potassium. In conclusion, the results provided strong evidence for benefits of combined inoculation with the indigenous ectomycorrhizal fungi on P. densiflora seedlings under controlled conditions.

  6. Influence of stand density on soil CO2 efflux for a Pinus densiflora forest in Korea.

    Science.gov (United States)

    Noh, Nam Jin; Son, Yowhan; Lee, Sue Kyoung; Yoon, Tae Kyung; Seo, Kyung Won; Kim, Choonsig; Lee, Woo-Kyun; Bae, Sang Won; Hwang, Jaehong

    2010-07-01

    We investigated the influence of stand density [938 tree ha(-1) for high stand density (HD), 600 tree ha(-1) for medium stand density (MD), and 375 tree ha(-1) for low stand density (LD)] on soil CO(2) efflux (R (S)) in a 70-year-old natural Pinus densiflora S. et Z. forest in central Korea. Concurrent with R (S) measurements, we measured litterfall, total belowground carbon allocation (TBCA), leaf area index (LAI), soil temperature (ST), soil water content (SWC), and soil nitrogen (N) concentration over a 2-year period. The R (S) (t C ha(-1) year(-1)) and leaf litterfall (t C ha(-1) year(-1)) values varied with stand density: 6.21 and 2.03 for HD, 7.45 and 2.37 for MD, and 6.96 and 2.23 for LD, respectively. In addition, R (S) was correlated with ST (R (2) = 0.77-0.80, P densiflora.

  7. [Genetic diversity and mating system Pinus brutia var. Stankewiczii sukacz. in small localities of Sudak (Crimea)].

    Science.gov (United States)

    Korshikov, I I; Kalafat, L A; Milchevskaya, Ya G

    2015-01-01

    A comparative analysis of genetic variation at 12 polymorphic isozyme loci, and the mating system has been carried out in mature trees and their seed progeny in three small localities of Pinus brutia var. stankewiczii Sukacz. near the town of Sudak--settlement of Novyi Svet in the Crimea. We found that embryos maintain the same allelic diversity as mother plants but their observed heterozygosity is lower on the average by 37.4%. The significant deviation of genotype distribution from the theoretically expected ratios caused by the deficiency of heterozygotes was observed at 8 out of 12 loci. Multilocus estimate of outcrossing rate (t(m)) in populations varied from 68.9 to 94.9% making on the average 80.7%.

  8. [Age dynamics of population gene pool of the Crimean pine (Pinus pallasiana D. Don) in Crimea].

    Science.gov (United States)

    Korshikov, I I; Mudrik, E A; Krasnoshtan, O V; Velikorid'ko, T I; Kalafat, L A; Podgornyĭ, D Iu

    2011-01-01

    Polymorphism of young (14-16 years), middle-aged (70-80 years) and old (120-150 years) plants and their seed embryos has been studied using 20 and 10 allozyme loci correspondingly in the population of Pinus pallasiana D. Don from Mountain Crimea. It was revealed that the old-aged trees had significantly lower level of expected heterozygosity than the young plants. The level of observed heterozygosity of embryos of the uneven-aged plants was slightly different among the embryo samples and significantly lower than in the samples of maternal trees. Supernumerary homozygotation of the embryos is caused by low level of cross-pollination in three studied samples of plants (t(m) = 0,537-0,637).

  9. [Genetic structure, subdivision, and population differentiation in Stankewiczii pine Pinus stankewiczii (Sukacz.) Fomin from Mountain Crimea].

    Science.gov (United States)

    Korshikov, I I; Gorlova, E M

    2006-06-01

    In order to analyze the genetic structure, subdivision and differentiation within and between two small isolated populations of the Crimea relict endemic, Pinus stankewiczii (Sukacz.) Fomin, electrophoretic analysis of the isozyme variation at nine enzymatic systems was carried out using 183 oldest trees. It was demonstrated that in populations of P. stankewiczii, 80% of the genes were in polymorphic state. Each tree was heterozygous at 19.1% loci, and at 21.6% loci in artificial 50-year-old plantation. The genetic structure of two populations was less differentiated (DN = 0.006), compared to their individual localities (DN = 0.008-0.009). Within-population subdivision of the diffusely dispersed populations was higher (FST-GST = 1.8-2.0%) than that of the populations themselves (0.8%).

  10. Chemical modification of coating of Pinus halepensis pollen by ozone exposure.

    Science.gov (United States)

    Naas, Oumsaad; Mendez, Maxence; Quijada, Melesio; Gosselin, Sylvie; Farah, Jinane; Choukri, Ali; Visez, Nicolas

    2016-07-01

    Pollen coating, located on the exine, includes an extractible lipid fraction. The modification of the pollen coating by air pollutants may have implications on the interactions of pollen with plant stigmas and human cells. Pinus halepensis pollen was exposed to ozone in vitro and the pollen coating was extracted with organic solvent and analyzed by GC-MS. Ozone has induced chemical changes in the coating as observed with an increase in dicarboxylic acids, short-chain fatty acids and aldehydes. 4-Hydroxybenzaldehyde was identified as the main reaction product and its formation was shown to occur both on native pollen and on defatted pollen. 4-Hydroxybenzaldehyde is very likely formed via the ozonolysis of acid coumaric-like monomers constitutive of the sporopollenin. Modification of pollen coating by air pollutants should be accounted for in further studies on effect of pollution on germination and on allergenicity.

  11. Low molecular weight carbohydrates in pine nuts from Pinus pinea L.

    Science.gov (United States)

    Ruiz-Aceituno, L; Ramos, L; Martinez-Castro, I; Sanz, M L

    2012-05-16

    Low molecular weight carbohydrates in pine nuts from Pinus pinea L. (n = 7) have been studied by gas chromatography-mass spectrometry as their trimethylsilyl oximes. Besides previously reported components, such as glucose, fructose, sucrose, and raffinose, several soluble carbohydrates have been identified for the first time in this product, including saccharides (galactose, maltose, and planteose) and cyclitols (pinitol, galactinol, galactopinitol A1, fagopyritol B1, and other glycosyl-inositols). Most abundant cyclitols were chiro-inositol, fagopyritol B1, and pinitol, with concentrations ranging from 126.7 to 222.1 mg (100 g)(-1), 94.2 to 177.1 mg (100 g)(-1), and 51.2 to 282.8 mg (100 g)(-1), respectively.

  12. Single nucleotide polymorphism discovery of Pinus radiata with chromosome walking PCR method

    Institute of Scientific and Technical Information of China (English)

    Wei LI; Hui LI; Xiaoyang CHEN; Harry WU

    2008-01-01

    In this paper, the basic principle of chromosome walking is presented and we used an actin gene of radiata pine (Pinus radiata) as an example to conduct upstream and downstream chromosome walking for EST sequences. The full genomic sequence (2154 bp) of the actin gene, including promoters 5' UTR, CDS and 3' UTR, was identified by chromosome walking. PCR amplification and DNA band sequencing from 200 unrelated radiata pine trees revealed a total of 21 SNPs for the actin gene, three in the promoter region, 15 in CDS and 4 in 3' UTR. The results of this experiment provide a technical framework for SNPs dis-covery in none coding regions of candidate genes.

  13. Environmental pollution changes in membrane lipids, antioxidants and vitality of Scots pine (Pinus sylvestris L. pollen

    Directory of Open Access Journals (Sweden)

    Paweł M. Pukacki

    2011-07-01

    Full Text Available Investigations were carried out on pollen grains of Scots pine (Pinus sylvestris L. collected from trees at 1.5, 3, 4 km and control, 20 km from the Luboń factory producing mineral fertilisers. The percentage of germination of pollen formed close to the pollution source was ca 20% lower compared to the control pollen. Lowered vitality of the pollen was effected in changes of the structure of cytoplasmic membranes. Pollen from the polluted area contained ca 15% less total phospholipids, mainly phosphatidylcholine and phosphatytidylinositol and had a lower content of soluble proteins and less of low molecular antioxidants, such as thiols and ascorbic acid. Composition of total fatty acid in phospholipids fractions showed a significant reduction in the degree of unsaturation of fatty acids. Pollen originating from the polluted area and stored at -30°C showed considerably stronger degradation of cytoplasmic membranes than control.

  14. The effects of understory vegetation on P availability in Pinus radiata forest stands:A review

    Institute of Scientific and Technical Information of China (English)

    A. Arivin Rivaie

    2014-01-01

    In many second-rotation Pinus radiata forest planta-tions, there has been a steady trend towards wider tree spacing and an increased rate of application of P fertiliser. Under these regimes, the potential for understory growth is expected to in-crease through increased light and greater nutrient resources. Therefore, understory vegetation could become a more signifi-cant component of P cycling in P. radiata forests than under closely-spaced stands. Studies have shown that growth rates and survival of trees is reduced in the presence of understory vegeta-tion due to the competition of understory vegetation with trees. Other studies have suggested that understory vegetation might have beneficial effects on nutrient cycling and conservation within forest stands. This review discusses the significance of understory vegetation in radiata pine forest stands, especially their role in enhancing or reducing P availability to forest trees.

  15. Antagonismo de Trichoderma SPP. E Bacillus subtilis (UFV3918 a Fusarium sambucinum em Pinus elliottii engelm

    Directory of Open Access Journals (Sweden)

    Caciara Gonzatto Maciel

    2014-06-01

    Full Text Available Pinus elliottii é uma espécie de importância no setor florestal e apresenta vulnerabilidade na qualidade sanitária de suas sementes, especialmente pela associação de Fusarium spp., responsável por perdas de plântulas no viveiro. Este trabalho teve como objetivo avaliar a ação antagonista in vitro e in vivo dos agentes Trichoderma spp. e Bacillus subtilis (UFV3918 no controle de Fusarium sambucinum, responsável por danos em plântulas de Pinus elliottii. O controle in vitro foi avaliado através da inibição do crescimento micelial (confronto pareado de culturas, após a incubação a 25±2 ºC e fotoperíodo de 12 h. Para os testes in vivo (desenvolvidos em condições de viveiro, as sementes inicialmente foram inoculadas com o patógeno e, na sequência, microbiolizadas com os agentes antagônicos, para posterior semeadura. Utilizaram-se as técnicas de contato com o biocontrolador em meio BDA por 48 h e peliculização, como formas de microbiolização. Tanto Trichoderma spp. quanto Bacillus subtilis (UFV3918 foram eficientes no controle in vitro de F. sambucinum, e no teste de biocontrole in vivo o produto Bacillus subtilis (UFV3918 destacou-se, reduzindo as perdas de plântulas causadas pelo patógeno, assim como potencializando as variáveis de comprimento de plântula, massa verde e massa seca.

  16. Analysis of mating system in two Pinus cembra L. populations of the Ukrainian Carpathians

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    Dmitri V. Politov

    2008-09-01

    Full Text Available In natural pine populations, a mixed mating system is typical,characterized by the proportions of selfed and outcrossed seeds. Swiss stone pine(Pinus cembra L. is one of the least studied European conifers in this respect. The mating system of six polymorphic allozyme loci were studied in haploid megagametophytes and diploid open-pollinated embryos in two stands located in theEast Carpathians. In the 'Gorgany' population (24 trees, 198 seeds the mean singlelocus estimated outcrossing rate (ts was 0.731, and the multilocus estimate (tm was 0.773. In the 'Yayko' population the outcrossing rate was lower (27 trees, 213 seeds, ts=0.645, tm=0.700, suggesting 23-30% of seeds are self-pollinated. Correlation ofoutcrossing rate estimates among loci was less than 1, (0.300 in 'Gorgany' and 0.469 in 'Yayko' indicating biparental inbreeding occurred. Differences between tm and ts (0.042 in 'Gorgany' and 0.056 in 'Yayko' can also be influenced by consanguineous mating, indicated by the presence of spatial and genetic family structure. In small isolated populations of Pinus cembra, which are typical for the Carpathian part of the species' range, inbreeding depression may negatively affect seed quality. The high proportion of selfed seeds observed here can be expected in any seedlot of this species and should be taken into account while planning gene conservation orreforestation measures. Maternal trees in these populations showed no heterozygote deficiency at these allozyme loci, and instead showed increased proportions of heterozygotes (inbreeding coefficient FIS = -0.200 in 'Gorgany' and -0.142 in 'Yayko'. Balancing selection may explain heterozygosity levels up to and above equilibrium proportions.

  17. Regeneration complexities of Pinus gerardiana in dry temperate forests of Indian Himalaya.

    Science.gov (United States)

    Kumar, Raj; Shamet, G S; Mehta, Harsh; Alam, N M; Kaushal, Rajesh; Chaturvedi, O P; Sharma, Navneet; Khaki, B A; Gupta, Dinesh

    2016-04-01

    Pinus gerardiana is considered an important species in dry temperate forests of North-Western Indian Himalaya because of its influence on ecological processes and economic dependence of local people in the region. But, large numbers of biotic and abiotic factors have affected P. gerardiana in these forests; hence, there is a crucial need to understand the regeneration dynamics of this tree species. The present investigation was conducted in P. gerardiana forests to understand vegetation pattern and regeneration processes on different sites in the region. Statistical analysis was performed to know variability in growing stock and regeneration on sample plots, while correlation coefficients and regression models were developed to find the relationship between regeneration and site factors. The vegetation study showed dominance of P. gerardiana, which is followed by Cedrus deodara, Pinus wallichiana and Quercus ilex in the region. The growing stock of P. gerardiana showed steep increasing and then steadily declining trend from lower to higher diameter class. The distribution of seedling, sapling, pole and trees was not uniform at different sites and less number of plots in each site were observed to have effective conditions for continuous regeneration, but mostly showed extremely limited regeneration. Regeneration success ranging from 8.44 to 15.93 % was recorded in different sites of the region, which suggests that in different sites regeneration success is influenced by collection of cone for extracting seed, grazing/browsing and physico-chemical properties of soil. Regeneration success showed significant correlation and relationship with most of abiotic and biotic factors. The regeneration success is lower than the requirement of sustainable forest, but varies widely among sites in dry temperate forests of Himalaya. More forest surveys are required to understand the conditions necessary for greater success of P. gerardiana in the region.

  18. The Pinus taeda genome is characterized by diverse and highly diverged repetitive sequences

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    Yandell Mark

    2010-07-01

    Full Text Available Abstract Background In today's age of genomic discovery, no attempt has been made to comprehensively sequence a gymnosperm genome. The largest genus in the coniferous family Pinaceae is Pinus, whose 110-120 species have extremely large genomes (c. 20-40 Gb, 2N = 24. The size and complexity of these genomes have prompted much speculation as to the feasibility of completing a conifer genome sequence. Conifer genomes are reputed to be highly repetitive, but there is little information available on the nature and identity of repetitive units in gymnosperms. The pines have extensive genetic resources, with approximately 329000 ESTs from eleven species and genetic maps in eight species, including a dense genetic map of the twelve linkage groups in Pinus taeda. Results We present here the Sanger sequence and annotation of ten P. taeda BAC clones and Genome Analyzer II whole genome shotgun (WGS sequences representing 7.5% of the genome. Computational annotation of ten BACs predicts three putative protein-coding genes and at least fifteen likely pseudogenes in nearly one megabase of sequence. We found three conifer-specific LTR retroelements in the BACs, and tentatively identified at least 15 others based on evidence from the distantly related angiosperms. Alignment of WGS sequences to the BACs indicates that 80% of BAC sequences have similar copies (≥ 75% nucleotide identity elsewhere in the genome, but only 23% have identical copies (99% identity. The three most common repetitive elements in the genome were identified and, when combined, represent less than 5% of the genome. Conclusions This study indicates that the majority of repeats in the P. taeda genome are 'novel' and will therefore require additional BAC or genomic sequencing for accurate characterization. The pine genome contains a very large number of diverged and probably defunct repetitive elements. This study also provides new evidence that sequencing a pine genome using a WGS approach is

  19. FAUNA DO SOLO EM ÁREAS COM Eucalyptus spp. E Pinus elliottii, SANTA MARIA, RS

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    Maria Izabel Jacques Soares

    2000-10-01

    Full Text Available Este trabalho, com início na primavera de 1996 e término no inverno de 1997, teve como objetivo realizar o levantamento populacional da fauna do solo em áreas com Eucalyptus spp. e Pinus elliottii, situadas no Campus da Universidade Federal de Santa Maria, Rio Grande do Sul, Brasil. Para a coleta do solo, utilizou-se um extrator, formando-se amostras em dez pontos, nas profundidades de 0-5, 5-10, 10-15 e de 15-20 cm, em cada estação do ano. Por meio deste estudo, pôde-se identificar alguns representantes dos Phylum Annelida e Arthropoda que participam do contexto fauna-solo-vegetação. O total de indivíduos, seu respectivo percentual e número de indivíduos por metro quadrado foram obtidos. Observou-se o predomínio do Phylum Arthropoda e, mais especificamente, da classe Insecta na área com Eucalyptus spp. e do Phylum Annelida na área com Pinus elliottii. A variável profundidade originou modelos matemáticos que explicam a distribuição populacional dentre as áreas estudadas. A variável estação do ano não apresentou uma diferença significativa. Não foi possível, portanto, estabelecer um modelo de distribuição populacional.

  20. Identification and persistence of Pinus pollen DNA on cotton fabrics: A forensic application.

    Science.gov (United States)

    Schield, Cassandra; Campelli, Cassandra; Sycalik, Jennifer; Randle, Christopher; Hughes-Stamm, Sheree; Gangitano, David

    2016-01-01

    Advances in plant genomics have had an impact on the field of forensic botany. However, the use of pollen DNA profiling in forensic investigations has yet to be applied. Five volunteers wore a jacket with Pinus echinata pollen-containing cotton swatches for a 14-day period. Pollen decay was evaluated at days 0, 3, 6, 9 and 14 by microscopy. Pollen grains were then transferred to slides using a portable forensic vacuum handle. Ten single grains per swatch were isolated for DNA analysis. DNA was extracted using a high throughput extraction method. A nine-locus short tandem repeat (STR) multiplex system, including previously published primers from Pinus taeda, was developed. DNA was amplified by PCR using fluorescent dyes and analyzed by capillary electrophoresis. Pollen counts from cotton swatches in a 14-day period exhibited an exponential decay from 100% to 17%. The success rate of PCR amplification was 81.2%. Complete and partial STR profiles were generated from 250 pollen grains analyzed (44% and 37%, respectively). Due to the limited amount of DNA, drop-in events were observed (1.87%). However, the rate of contamination with pollen from other pine individuals originating from environmental sources was 4.4%. In conclusion, this study has shown that pollen can be a stable source of forensic DNA evidence, as a proof-of-principle, and that may persist on cotton clothing for at least 14 days of wear. This method can be applied in forensic cases where pollen grains larger than 10 μm (e.g., from herbs or trees) may be transferred to clothing (worn by suspect or victim) by primary contact.

  1. The gymnosperm Pinus pinea contains both AOX gene subfamilies, AOX1 and AOX2.

    Science.gov (United States)

    Frederico, António Miguel; Zavattieri, Maria Amely; Campos, Maria Doroteia; Cardoso, Hélia Guerra; McDonald, Allison E; Arnholdt-Schmitt, Birgit

    2009-12-01

    The gymnosperm Pinus pinea L. (stone pine) is a typical Mediterranean pine used for nuts and timber production, and as an ornamental around the world. Pine genomes are large in comparison to other species. The hypothesis that retrotransposons, such as gymny, made a large contribution to this alteration in genome size was recently confirmed. However, P. pinea is unique in other various aspects. P. pinea demonstrates a different pattern of gymny organization than other Pinus subgenera. Additionally, P. pinea has a highly recalcitrant behaviour in relation to standard conifer protocols for the induction of somatic embryogenesis or rooting. Because such types of cell reprogramming can be explained as a reaction of plant cells to external stress, it is of special interest to study sequence peculiarities in stress-inducible genes, such as the alternative oxidase (AOX). This is the first report containing molecular evidence for the existence of AOX in gymnosperms at the genetic level. P. pinea AOXs were isolated by a polymerase chain reaction (PCR) approach and three genes were identified. Two of the genes belong to the AOX1 subfamily and one belongs to the AOX2 subfamily. The existence of both AOX subfamilies in gymnosperms is reported here for the first time. This discovery supports the hypothesis that AOX1 and AOX2 subfamilies arose prior to the separation of gymnosperms and angiosperms, and indicates that the AOX2 is absent in monocots because of subsequent gene loss events. Polymorphic P. pinea AOX1 sequences from a selected genetic clone are presented indicating non-allelic, non-synonymous and synonymous translation products.

  2. [Photosynthetic functions and chlorophyll fast fluorescence characteristics of five Pinus species].

    Science.gov (United States)

    Qiu, Nian-Wei; Zhou, Feng; Gu, Zhu-Jun; Jia, Shu-Qin; Wang, Xing-An

    2012-05-01

    A comparative study was made on the needle morphological characteristics, photosynthetic rate, and chlorophyll fast fluorescence induction curves of five representative Pinus species P. parvifiora, P. armandii, P. bungeana, P. tabuliformis, and P. densiflora. Significant differences were observed in the needle morphological characteristics among the five species. P. tabuliformis had the longest needle length and highest needle density, whereas P. bungeana had the highest chlorophyll content. P. densiflora and P. parvifiora had the maximum and minimum photosynthetic rate, respectively. There was a positive correlation between the photosynthetic rate and stomatal conductance across the five species. The differences in the chlorophyll fast fluorescence induction curves of the five species were mainly manifested in J-step and I-step. Although the five species had similar values of Fv/Fm, Fv/Fo and Tfm, P. parviflora had significantly higher values of dV/dt(o), dVG/d(o), V and Vi, but lower energy flux ratio psi(o), phiEo and phiRo, compared with the other four species. The low PSII activity and efficiency of P. parviflora might relate to its smallest Sm, Sm/Tfm and N. P. densiflora and P. parvifiora had the maximum and minimum vitality indices PI(ABS/CSo/CSm) and DF, respectively, and there existed significant positive correlations between the PI(CSo) and PI(CSm) and the net photosynthetic rate of the five species, suggesting that PI(CSo) and PI(CSm) could be used to estimate the photosynthetic activity of Pinus trees.

  3. Consequences for selected high-elevation butterflies and moths from the spread of Pinus mugo into the alpine zone in the High Sudetes Mountains

    OpenAIRE

    Bílá, Karolína; Šipoš, Jan; Kindlmann, Pavel; Kuras, Tomáš

    2016-01-01

    Due to changes in the global climate, isolated alpine sites have become one of the most vulnerable habitats worldwide. The indigenous fauna in these habitats is threatened by an invasive species, dwarf pine (Pinus mugo), which is highly competitive and could be important in determining the composition of the invertebrate community. In this study, the association of species richness and abundance of butterflies with the extent of Pinus mugo cover at individual alpine sites was determined. Butt...

  4. Modelos de afilamento para o sortimento do fuste de Pinus taeda L Taper function for assortment of Pinus taeda L. stem

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    Carlos Alberto Martinelli de Souza

    2008-12-01

    Full Text Available Este trabalho teve como objetivo avaliar diferentes modelos de afilamento do fuste de Pinus taeda para estimar variáveis de interesse ao longo do fuste. Foram avaliadas as funções propostas por Anony, Kozak, Munro, Silva & Sterba, Prodan e o Polinômio de 5° grau. O estudo teve como base dados de cubagem rigorosa de 68 árvores, provenientes de plantios com idade de 25 anos, pertencentes à empresa Florestal Gateados Ltda, localizada no município de Campo Belo do Sul, Santa Catarina (SC, Brasil. A partir das estatísticas coeficiente de determinação ajustado (R²aj, erro padrão das estimativas (Syx, desvio médio, média das diferenças (md e desvio padrão das diferenças (dpd foi selecionado o Polinômio do 5° grau para estimar as variáveis ao longo do fuste, como a altura comercial, o volume comercial, os diâmetros e os volumes das 1ª e 2ª toras. Em relação à altura comercial, a equação proveniente do modelo escolhido apresentou resultados sem tendências e com erros oscilando em intervalo pequeno. Em se tratando do volume comercial, pode ser observada certa tendência em subestimativas para as árvores com menores DAP e uma leve tendência para aquelas árvores com maior DAP. Em relação ao diâmetro e ao volume da 1a tora, houve superestimativa dos valores, mas com erros baixos, principalmente para o diâmetro. Para as estimativas do diâmetro e do volume da 2ª tora, os resultados foram melhores do que os encontrados na 1a tora, com leve tendência em subestimar o diâmetro e, praticamente, sem tendência para o volume.This research had as objective to evaluate different taper models for Pinus taeda and select the best one to estimate variables of interest throughout the stem. The functions proposed by Anony, Kozak, Munro, Silva & Sterba, Prodan and the 5° degree Polynomial were evaluated. With data from rigorous cubage of 68 trees, with 25 years old, coming from plantation of Florestal Gateados Ltda, located, in the

  5. Avaliação e seleção precoce para crescimento de Pinus taeda Early evaluation and selection for growth in Pinus taeda

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    Estefano Paludzyszyn Filho

    2002-12-01

    Full Text Available O objetivo deste trabalho foi avaliar a eficiência de diferentes métodos de seleção quanto aos caracteres ligados ao crescimento do fuste e o potencial da seleção precoce de genitores. Em 48 progênies de meios-irmãos de Pinus taeda L., foi medido o diâmetro do fuste a 30 cm e a 1,30 m do solo, aos 16 e 84 meses de idade, além da altura total. As progênies foram cultivadas em espaçamento tradicional e reduzido. Os componentes de variância, parâmetros e valores genéticos aditivos foram obtidos a partir de um modelo de análise individual. A seleção indireta foi de eficiência similar à direta quanto ao diâmetro (93% e menor quanto à altura (78%. A seleção seqüencial foi 16% (altura e 28% (diâmetro mais eficiente que a seleção única. A eficiência da seleção combinada foi maior em relação a direta para diâmetro. Vinte genitores selecionados por valores genéticos aditivos quanto ao diâmetro, aos 16 e 84 meses, aumentaram a média em 7,6% e 8,1%, respectivamente. A alta magnitude da correlação genética no que diz respeito ao diâmetro entre idades revelou que esse caráter, avaliado aos 16 meses de idade, prediz o crescimento aos 84 meses. Isso reduz custos de avaliação de progênies, antecipa o desbaste de genitores e a oferta de sementes melhoradas.The objective of this work was to evaluate the efficiency of different methods of selection in growth traits and early selection. Individual total height and stem diameter at 30 cm above the ground in close-spaced early genetic evaluation at 16 months were assessed. In a 84 month sib cohort, as a comparison, height and diameter at breast height were evaluated. Phenotypic and genetic parameters for height and diameter were estimated for 48 open-pollinated families of loblolly pine (Pinus taeda L. planted in Southern Brazil using an individual tree model. In this retrospective study, the indirect selection was of similar effectiveness as direct selection in stem

  6. Delimitação entre os lenhos juvenil e adulto de Pinus elliottii engelm Delimitation the juvenile and mature wood of Pinus elliottii engelm

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    Gilmara Pires de Moura Palermo

    2013-02-01

    Full Text Available Durante o crescimento, as árvores produzem diferentes tipos de tecido lenhoso. O xilema produzido nos primeiros anos até certa idade cambial é denominado lenho juvenil e apresenta propriedades físicas, mecânicas, químicas e anatômicas diferentes do xilema produzido após certa idade cambial, isto é, quando as células do câmbio já amadureceram. Dessa forma, um prévio conhecimento sobre a qualidade da matéria-prima produzida é de fundamental importância para melhor aplicabilidade do material. Com base nesse contexto, este trabalho objetivou, a partir de dados de comprimento de traqueídeos e densidade da madeira, delimitar a idade de transição entre os lenhos juvenil e adulto. Para isso, coletaram-se três árvores de Pinus elliottii var. elliottii Engelm., com 35 anos de idade, provenientes da Estação Experimental de Itapetininga, localizada em São Paulo, Brasil. De cada árvore, retiraram-se discos de aproximadamente 5 cm de espessura, extraídos a 0,05 m do solo, que foram utilizados para determinação radial da densidade pelo método de atenuação da radiação gama e comprimento dos traqueídeos. Os resultados da análise de regressão linear indicaram que o lenho juvenil está limitado aos sete primeiros anos de crescimento da árvore e o lenho adulto é formado após os 20 anos de idade. No lenho adulto há diferenças significativas entre as idades médias obtidas através do comprimento dos traqueídeos e da densidade da madeira.Trees produce different types of woody tissue during growth. The xylem produced in the first years until a certain cambial age is called juvenile wood and has different physical, mechanical, chemical and anatomical characteristics from that produced later, when the cambial cells have matured. Thus, prior knowledge of the quality of the wood is of fundamental importance for choosing the best use of the material. This work aimed at using data from length of the tracheid and wood density to

  7. 毛乌素沙地樟子松和油松人工林光合生理特性%The Photosynthetic Characteristics of Pinus Sylvestris var, Mongolica and Pinus Tabulae formis in Mu Us Sandland

    Institute of Scientific and Technical Information of China (English)

    丁晓纲; 何茜; 李吉跃; 张方秋; 刘育贤

    2011-01-01

    In the growing season, the daily gas exchange, water use efficiency of Pinus sylvestris var. mongolica and Pinus tabulae formis in Mu Us sandland were measured with a portable Li-6400 gas analysis system under natural conditions. The results showed that the diurnal courses of net photosynthetic rate and transpiration rate display a singal-peak pattern. Water use efficiency culminates in the early morning. The maximum and the average value of the net photosynthesis of the Pinus sylvestris var. mongolica is larger than that of the Pinus tabulaeformis. The average value of the water use efficiency of Pinus sylvestris var.mongolica is 3. 63 tμmolCO2/mmolH2O, which was higher than that of Pinus tabulaeformis (3. 22 μmol CO2/mmolH2O). Regression analysis showed that the net photosynthetic rate of Pinus sylvestris var. mongolica was controlled by PAR, Gs and Ci, while for Pinus tabulaeformis was PAR and Gs.%利用Li-6400便携式气体分析系统,在自然条件下对毛乌素沙地腹地樟子松和油松人工林生长季叶片气体交换、水分利用效率及相关环境因子进行测定,同时探讨了影响樟子松、油松光合速率的内外因素.结果表明:生长季内毛乌素地区油松和樟子松的光合速率、蒸腾速率都表现出一定的日变化规律,都呈单峰型变化曲线;6月、7月、8月针叶树水分利用效率曲线呈下降趋势,上午时段的水分利用效率明显高于下午时段的水分利用效率.5月和9月针叶树水分利用效率日变化呈"W"型曲线,樟子松水分利用效率生长季均值为3.6μcmo1O0 /mmo1HO0,油松水分利用效率生长季均值为3.22μmmolCO2/mmolH2O,樟子松的水分利用效率始终高于油松.樟子松的净光合速率主要受光合有效辐射、气孔导度和胞间CO2浓度的影响,限制油松净光合速率的主要因子是光合有效辐射PAR和气孔导度.

  8. Pinus afforestation in South Brazilian highlands: soil chemical attributes and organic matter composition Florestamento com Pinus em solos de altitude do Sul do Brasil: atributos químicos e matéria orgânica do solo

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    Deborah Pinheiro Dick

    2011-04-01

    Full Text Available In the last three decades, exotic tree species are being introduced in the natural pastures of the highlands located at the northeastern part of Rio Grande do Sul State (RS, Brazil. This alteration of land use may impart drastic changes in the soil attributes. In this context, this work aimed to evaluate the impact of Pinus taeda afforestation on soil chemical attributes and organic matter (SOM composition in Leptosols from Campos de Cima da Serra, RS. Soil samples under eight year old (Pi8 and 30 year old (Pi30 Pinus plantations and under native pasture (NP were studied. Contents of exchangeable cations and of micronutrients and soil pH were determined. The SOM composition was investigated by means of elemental analyses and FTIR spectroscopy. The soil under pasture had a higher content of nutrients and of SOM in comparison to Pinus soils, reflecting the higher input and decomposition rate of the below ground added residue in the grassland environment. The SOM in pasture soils showed a higher content of carbohydrate and of structures derived from microbial metabolism. Besides the depletion of nutrients and of SOM, Pinus afforestation affected the SOM quality: following afforestation, the proportion of chemically recalcitrant structures and of carboxylic groups increased, whereas N-containing groups decreased.Nas três últimas décadas, o cultivo de espécies exóticas vem sendo introduzido nas áreas de pastagem de solos de altitude localizados na região nordeste do Estado do rio Grande do Sul. Essa alteração de uso do solo pode causar mudanças drásticas nos atributos do solo. Avaliou-se o impacto do florestamento com Pinus Taeda nos atributos químicos e na composição da matéria orgânica (MOS de Neossolos Litólicos dos Campos de Cima da Serra, RS. Foram estudadas amostras de solo sob plantação de Pinus há oito (Pi8 e há 30 anos (Pi30 e sob pastagem natural (NP, sendo determinados os teores de cátions trocáveis e de

  9. Consequences for selected high-elevation butterflies and moths from the spread of Pinus mugo into the alpine zone in the High Sudetes Mountains

    Directory of Open Access Journals (Sweden)

    Karolína Bílá

    2016-06-01

    Full Text Available Due to changes in the global climate, isolated alpine sites have become one of the most vulnerable habitats worldwide. The indigenous fauna in these habitats is threatened by an invasive species, dwarf pine (Pinus mugo, which is highly competitive and could be important in determining the composition of the invertebrate community. In this study, the association of species richness and abundance of butterflies with the extent of Pinus mugo cover at individual alpine sites was determined. Butterflies at alpine sites in the High Sudetes Mountains (Mts. were sampled using Moericke yellow water traps. The results of a Canonical Correspondence Analysis (CCA indicated that at a local scale the area of alpine habitats is the main limiting factor for native species of alpine butterflies. Butterfly assemblages are associated with distance to the tree-line with the optimum situated in the lower forest zone. In addition the CCA revealed that biotic factors (i.e. Pinus mugo and alpine tundra vegetation accounted for a significant amount of the variability in species data. Regionally, the CCA identified that the species composition of butterflies and moths is associated with presence and origin of Pinus mugo. Our study provides evidence that the structure of the Lepidopteran fauna that formed during the postglacial period and also the present composition of species assemblages is associated with the presence of Pinus mugo. With global warming, Pinus mugo has the potential to spread further into alpine areas and negatively affect the local species communities.

  10. Consequences for selected high-elevation butterflies and moths from the spread of Pinus mugo into the alpine zone in the High Sudetes Mountains.

    Science.gov (United States)

    Bílá, Karolína; Šipoš, Jan; Kindlmann, Pavel; Kuras, Tomáš

    2016-01-01

    Due to changes in the global climate, isolated alpine sites have become one of the most vulnerable habitats worldwide. The indigenous fauna in these habitats is threatened by an invasive species, dwarf pine (Pinus mugo), which is highly competitive and could be important in determining the composition of the invertebrate community. In this study, the association of species richness and abundance of butterflies with the extent of Pinus mugo cover at individual alpine sites was determined. Butterflies at alpine sites in the High Sudetes Mountains (Mts.) were sampled using Moericke yellow water traps. The results of a Canonical Correspondence Analysis (CCA) indicated that at a local scale the area of alpine habitats is the main limiting factor for native species of alpine butterflies. Butterfly assemblages are associated with distance to the tree-line with the optimum situated in the lower forest zone. In addition the CCA revealed that biotic factors (i.e. Pinus mugo and alpine tundra vegetation) accounted for a significant amount of the variability in species data. Regionally, the CCA identified that the species composition of butterflies and moths is associated with presence and origin of Pinus mugo. Our study provides evidence that the structure of the Lepidopteran fauna that formed during the postglacial period and also the present composition of species assemblages is associated with the presence of Pinus mugo. With global warming, Pinus mugo has the potential to spread further into alpine areas and negatively affect the local species communities.

  11. Primer registro de Naupactus ruizi (Coleoptera: Curculionoidea asociado con Pinus ponderosa (Gymnospermae: Pinaceae en Patagonia First record of Naupactus ruizi (Coleoptera: Curculionoidea associated to Pinus ponderosa (Gymnospermae: Pinaceae in Patagonia

    Directory of Open Access Journals (Sweden)

    Cecilia A. Gómez

    2006-07-01

    Full Text Available Naupactus ruizi (Brèthes es un gorgojo de rostro corto, distribuido en la Argentina y Chile, que habita en ambientes áridos asociado con vegetación xerofítica y alcanza el rango más austral entre los miembros de la tribu Naupactini. Un relevamiento de insectos y de patógenos como plagas potenciales, realizado durante el 2005 en plantaciones de Pinus spp. en la Patagonia andina argentina, resultó en el hallazgo de adultos de N. ruizi que se alimentaban de acículas de Pinus ponderosa Dougl. ex Laws. Este hallazgo es sorprendente dado que las especies de Naupactini, consumen casi exclusivamente angiospermas. Interpretamos que el cambio de huésped, habría ocurrido como consecuencia de una colonización reciente favorecida por la amplia distribución geográfica del gorgojo y su capacidad para sobrevivir en hábitats marginales, donde probablemente las plantas nativas son escasas y el nuevo huésped presenta una gran abundancia local.Naupactus ruizi (Brèthes is a broad-nosed weevil recorded from Argentina and Chile , that inhabits arid environments with xerophitic vegetation and reaches the southern most distribution of the tribe Naupactini. A survey of potential insect pests and pathogens of plantations of Pinus spp. and other forest species, conducted during 2005 in Argentinean Patagonian Andean forestations, allowed to find N. ruizi feeding on pine needles of Pinus ponderosa Dougl. ex Laws. This finding was unexpected, since species of Naupactini consume almost exclusively angiosperms. We interpret that the new host association may represent a recent host shift, probably facilitated by the broad range of the weevil, its capacity to survive in marginal habitats where natural hosts are scarce, and the local abundance of the new host.

  12. Análise da madeira do Pinus oocarpa parte II: caracterização estrutural da lignina de madeira moída Chemical analysis of the Pinus oocarpa wood. Part II: characterization of the milled wood lignin

    Directory of Open Access Journals (Sweden)

    Sérgio Antônio Lemos de Morais

    2005-06-01

    Full Text Available Este estudo caracterizou a Lignina de Madeira Moída (LMM proveniente de Pinus oocarpa cultivado na região do Cerrado brasileiro. A LMM foi isolada e analisada por meio das espectrometrias no infravermelho com transformada de Fourier (IVTF, de ressonância magnética nuclear do próton e carbono-13 e por intermédio de métodos químicos de análise por via úmida. A LMM apresentou uma fórmula mínima igual a C9H9,2O2,6(OCH 30,8 e massas molares médias em massa (Mw e numérica (Mn de 3.969 e 1.133 Da, respectivamente. A LMM dessa madeira se enquadra dentro das ligninas típicas de coníferas.This work presents the characterization of the milled wood lignin (MWL of the Pinus oocarpa cultivated in the Brazilian cerrado. FTIR, carbon-13 and proton NMR spectroscopies as well as wet chemical methods were used. The established C9 unit formula for MWL was C9H9,2O2,6(OCH 30,8 and its relative molecular weights (Mw and (Mn were 3969 and 1133 Da, respectively. Pinus oocarpa MWL was typical of softwood lignins.

  13. Sensitive and specific detection of pine nut (Pinus spp.) by real-time PCR in complex food products.

    Science.gov (United States)

    Garino, Cristiano; De Paolis, Angelo; Coïsson, Jean Daniel; Bianchi, Daniela Manila; Decastelli, Lucia; Arlorio, Marco

    2016-03-01

    Pine nuts are a known source of food allergens and several cases of adverse immunological reaction after ingestion have been reported. To protect allergic consumers, methods to unequivocally detect the presence of pine nuts in complex matrices must be developed. A Taqman-based real time PCR method for the detection of Pinus spp. was set up. A homemade pesto spiked at known concentration of pine nut powder was used as model food. Moreover, DNA was purified from commercial foods declaring or not the presence of pine nuts. The method displayed a very high efficiency and specificity for the genus Pinus. The intrinsic LOD was 1pg of DNA, while the practical LOD evaluated on model foods was 0.1ppm of pine nuts powder, the lowest ever registered for the detection of food allergens via real-time PCR. Finally, the declared presence/absence of pine nut in commercial foods was confirmed.

  14. Control technique of sphaeropsis shoot blight in Pinus teada%火炬松枯梢病防治技术

    Institute of Scientific and Technical Information of China (English)

    沈友爱

    2001-01-01

    Sphaeropsis shoot blight in Pinus teada is a serious disease in south of China.The control experiments of cutting blight shoots,applying fertilizer and spraying fungicide were made ,The result shows that the above measures combined with spraying 50% Carbendazim wp could reach an efficacy of 88.6%.%火炬松枯梢病Sphaeropsis sapinea是为害火炬松Pinus taeda的毁灭性病害。该文通过剪枯梢、施肥和喷药相结合进行防治试验,结果表明:以剪枯梢为主,结合施肥或喷50%多菌灵的防治措施效果较好,防治率88.6%。

  15. FINE ROOT QUANTIFICATION IN A Pinus taeda L. STAND AND IN GRASSLAND AREA IN CAMBARÁ DO SUL (RS

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    Vicente Guilherme Lopes

    2010-12-01

    Full Text Available The objectives of this study were to comparatively quantify length and biomass of fine roots (≤ 2.0 mm in the soil profile (0-10, 10-20, 20-30, 30-40 cm  and in the litter of a 15-year-old Pinus taeda L. stand, as well as in an adjacent Grassland area. The samples were obtained through monolith excavation. Roots were separated through washing and collecting and were then distributed over a white sheet of paper, where images were obtained with a digital camera. Using the software Image Tool for Windows version 3.00© the images were processed to quantify root length. Subsequently, roots were dried in a stove and, weighed to determine the biomass. The vegetation in the Grassland area showed 234.28% greater density of fine roots than the adjacent area where the Pinus taeda L.stand is located.

  16. Effects of heavy metal pollution on genetic variation and cytological disturbances in the Pinus sylvestris L. population.

    Science.gov (United States)

    Prus-Głowacki, Wiesław; Chudzińska, Ewa; Wojnicka-Połtorak, Aleksandra; Kozacki, Leon; Fagiewicz, Katarzyna

    2006-01-01

    This isoenzymatic and cytogenetic study has shown significant differences in genetic composition between two groups of Pinus sylvestris trees: tolerant and sensitive to heavy metal pollution. Total and mean numbers of alleles and genotypes per locus were higher in the pollution-sensitive group of trees, but heterozygosity (Ho) was lower in this group. Fixation index (F) indicates that trees tolerant for pollution were in the Hardy-Weinberg equilibrium, while the sensitive group had a significant excess of homozygosity. Cytological analyses demonstrated numerous aberrations of chromosomes in meristematic root tissue of seedlings developed from seeds collected from trees in the polluted area. The aberrations included chromosome bridges and stickiness, laggards, retarded and forward chromosomes, and their fragments. The mitotic index was markedly lower in this group of seedlings, as compared to the control. Both isoenzymatic and cytological analyses showed a significant influence of heavy metal ions on the genetic structure of the Pinus sylvestris population.

  17. Investigation of Pinus mugo essential oil oxygenated fraction by combined use of gas chromatography and dry column chromatography.

    Science.gov (United States)

    A, M B; Coran, S A; Giannellini, V; Vincieri, F F; Moneti, G

    1981-09-01

    The oxygenated compounds of Pinus mugo Turra essential oil were investigated by a combination of GC and dry column chromatography (DCC) coordinated by GC data processing. The collected data resulted in a bar graph ("normalized" gas chromatogram) giving the RRT's and relative amounts of 68 components; 38 of them were identified by MS and IR. The described procedure may be used for essential oil analysis in general.

  18. Different patterns of genetic structure of relict and isolated populations of endangered peat-bog pine (Pinus uliginosa Neumann).

    Science.gov (United States)

    Wachowiak, W; Prus-Glowacki, W

    2009-01-01

    Recent changes in environmental conditions in populations of peat-bog pine (Pinus uliginosa Neumann) caused rapid decline or even extinction of the species in several stands in Central Europe. Conservation strategies for P. uliginosa require information about the evolutionary history and genetic structure of its populations. Using isozymes we assessed the genetic structure of P. uliginosa from four isolated stands in Poland and compared the results to genetic structures of other closely related pine species including eight populations of Pinus mugo, ten of Pinus sylvestris and one of Pinus uncinata. The level of genetic variability of P. uliginosa measured by the mean number of alleles per locus and average heterozygosity was similar to others related to P. uliginosa taxa from the reference group but it differs among populations. High genetic similarity was found between two populations of P. uliginosa from Low Silesian Pinewood. The populations were genetically distinct as compared to other populations including locus classicus of the species from the peat bog at Batorów Reserve. Very low genetic distance (DN = 0.002) and small genetic differentiation (GST = 0.003) were found between P. uliginosa and P. mugo in the sympatric populations of the species from Zieleniec peat bog suggesting the ongoing natural hybridisation and genetic contamination of peat-bog pine from this area. Some evidence for skew in allele frequency distribution potentially due to recent bottleneck was found in population from Low Silesian Pinewood. The analysed open pollinated progeny derived from two P. uliginosa stands from Low Silesian Pinewood showed the excess of homozygotes as compared to the maternal trees indicating high level of inbreeding (F = 0.105, F = 0.081). The results are discussed in the context of evolution of P. uliginosa populations, taxonomic relationships between the analysed species and conservation strategies for active protection of peat-bog pine.

  19. Plant communities with Pinus mugo (alliance Pinion mugo) in the subalpine belt of the Western Carpathians - a numerical approach

    OpenAIRE

    Jozef Šibík; Milan Valachovič; Ján Kliment

    2011-01-01

    A syntaxonomical revision of plant communities with dominant Pinus mugo in the Western Carpathians is presented. The data set of 341 relevés was examined and analysed using the detrended correspondence analysis and the cluster analysis. Major gradients and clusters were interpreted using Ellenberg’s indicator values. The major gradient in species composition was associated with available nutrients and moisture. The authors suggest distinguishing the dwarf pine stands of the supramontanous and...

  20. Effect of benfluralin on Pinus pinea seedlings mycorrhized with Pisolithus tinctorius and Suillus bellinii – Study of plant antioxidant response

    OpenAIRE

    Franco, A. R.; Pereira, Sofia I. A.; Castro, Pauia M. L.

    2015-01-01

    In this study, Pinus pinea seedlings mycorrhized with selected ectomycorrhizal fungi (ECMF), Pisolithus tinctorius and Suillus bellinii, were exposed to the herbicide benfluralin. Non-mycorrhized P. pinea seedlings and seedlings mycorrhized with ECMF were transferred to benfluralin-spiked soils at levels of 0.165, 1.65 and 16.5 mg kg 1. Plant growth and the fungal role on plant antioxidant response were assessed. In the presence of benfluralin, higher plant growth was observed in ...

  1. Effects of Restoration Techniques on Soil Carbon and Nitrogen Dynamics in Florida Longleaf Pine (Pinus palustris) Sandhill Forests

    OpenAIRE

    Martin Lavoie; Mack, Michelle C; John K. Hiers; Scott Pokswinski; Analie Barnett; Louis Provencher

    2014-01-01

    Historic fire suppression and intensive forest management in longleaf pine (Pinus palustris) sandhill forests has resulted in hardwood encroachment and degradation of this fire-dependent ecosystem. Active management is now required to restore native community structure and composition, but little is known about the long-term impacts of typical restoration techniques on ecosystem properties. In 1994, the Longleaf Pine Restoration Project (LPRP) was established in fire-excluded longleaf pine sa...

  2. SOIL SEEDBANK FROM THE UNDERSTOREY OF Pinus AND Eucalyptus IN THE FLONA DE BRASÍLIA, BRAZIL

    Directory of Open Access Journals (Sweden)

    Augusta Rosa Gonçalves

    2008-03-01

    Full Text Available An analysis of the soil seedbank from the understory of Pinus and Eucalyptus in the Floresta Nacional de Brasília wasperformed, these plantations were established in the 1980s, and since 1987 have not had adequate management. Two stands of each genuswere chosen, with and without gaps. In each one of these stands 15 plots of 2 x 2 m were implanted, where a sample of 0,30 m x 0,30 mof soil and two sub-sample of 0,125 x 0,15 m of litter were collected for a quantative and qualitative analyzis of the seedbank. The mediumdensity of seedlings emergence in the Pinus stand was 3.098,19 seeds.m-2 while for the Eucalyptus it was of 2.077,19 seeds.m-2, whichmeans that the seedbank from the Pinus understory area has a higher regeneration potential than the Eucalyptus plantations. The Pinusplantations contained in the understorey: 12 families, 25 genus and 39 species, while in the Eucalyptus: 14 families, 31 genus and 48species, proving more richness in the Eucalyptus seedbank. In both genuses the most important families were Poaceae and Asteraceae.Both Pinus and Eucalyptus seedbanks populations, with or without openings, proved that the more interference in its population higheris the number of seeds from herbaceous species in comparison to tree species. The Sørensen similarity índex applied to analyze theseedbanks in the four areas, both for soil fractions and litter, was low, 0,5 and 0, 486, respectively.

  3. Soil Nutrient, Woody Understory and Shoot and Root Growth Responses of Pinus brutia Ten. Saplings to Fire

    OpenAIRE

    Petros GANATSAS; Spanos, Ioannis; Marianthi TSAKALDIMI; Gerasimos GOUDELIS

    2012-01-01

    Nine years after a wildfire, above- and below-ground morphology of Pinus brutia Ten. saplings and the microsite factors prevailing in understorey (unburned area) and postfire conditions (burned area) of the Forest Park of Thessaloniki were studied. Major stand characteristics (density, tree canopy cover, tree height, crown height, and diameter) were measured in the unburned area. Light and soil conditions as well as plant cover of woody species were recorded in both areas (burned and unburned...

  4. 樟子松温棚容器育苗技术%Container Seedling Technique on Pinus sylvestris in Greenhouses

    Institute of Scientific and Technical Information of China (English)

    李得俊

    2012-01-01

    Container seedlings in greenhouses of Pinus sylvestris,improve the environment for the growth and shorten the breeding time,the seedlings of good quality,high survival rate.%温棚樟子松容器育苗,改善了生长环境,缩短了育苗时间,苗木质量好,造林成活率高。

  5. Exploration on Propagation Technology of Pinus sylvestris var.mongolica%樟子松繁殖技术探究

    Institute of Scientific and Technical Information of China (English)

    岳一江

    2015-01-01

    This paper research the biological characteristics and breeding technology of Pinus sylvestris var. mongolica (seedbed preparation, seed treatment, sowing, seedling management).%文章对樟子松的生物学特性和樟子松的繁殖技术(播种苗床准备、种子处理、播种操作、苗期管理)进行应用研究。

  6. Inferring Multiple Refugia and Phylogeographical Patterns in Pinus massoniana Based on Nucleotide Sequence Variation and DNA Fingerprinting

    OpenAIRE

    Xue-Jun Ge; Tsai-Wen Hsu; Kuo-Hsiang Hung; Chung-Jian Lin; Chi-Chung Huang; Chao-Ching Huang; Yu-Chung Chiang; Tzen-Yuh Chiang

    2012-01-01

    BACKGROUND: Pinus massoniana, an ecologically and economically important conifer, is widespread across central and southern mainland China and Taiwan. In this study, we tested the central-marginal paradigm that predicts that the marginal populations tend to be less polymorphic than the central ones in their genetic composition, and examined a founders' effect in the island population. METHODOLOGY/PRINCIPAL FINDINGS: We examined the phylogeography and population structuring of the P. massonian...

  7. Effects of climate variables on intra-annual stem radial increment in Pinus cembra (L.) along the alpine treeline ecotone

    OpenAIRE

    Gruber, Andreas; Zimmermann, Jolanda; Wieser, Gerhard; Oberhuber, Walter

    2009-01-01

    Within the alpine treeline ecotone tree growth is increasingly restricted by extreme climate conditions. Although intra-annual stem growth recorded by dendrometers can be linked to climate, stem diameter increments in slow-growing subalpine trees are masked by changes in tree water status.We tested the hypothesis that intra-annual radial stem growth in Pinus cembra is influenced by different climate variables along the treeline ecotone in the Austrian Alps. Dendrometer traces were compared wi...

  8. Impacts of more frequent droughts on a relict low-altitude Pinus uncinata stand in the French Alps

    OpenAIRE

    2015-01-01

    Cold microclimatic conditions provide exceptional microhabitats to Pinus uncinata stands occurring at abnormally low altitudes in seven paleorefugia of the northern French Alps. Here, P. uncinata is located at the lower bounds of its ecological limits and therefore expected to provide a sensitive indicator of climate change processes. We used dendrochronological analysis to study the growth patterns of closely spaced chronologies across an elevational transect and compare a relict low-altitud...

  9. Changes in Germination and Physiological Characteristics of Seeds of Robinia pesudoacacia and Pinus tabulaeformis during Artificial Ageing

    Institute of Scientific and Technical Information of China (English)

    2008-01-01

    In order to study seed quality changes of main afforestation species under high temperature and high relative humidity, the deterioration mechanism of seeds of Robinia pesudoacacia and Pinus tabulaeformis from arid and semiarid areas of Northern China was elucidated in this study. The seeds were artificially aged for 2 and 6 d at the temperature of 45 oC and the relative humidity (RH) of 50%, 75% and 100% , respectively. The results showed that the germinability decreased and the cell membrane deteriorated ...

  10. Holocene variability in the range distribution and abundance of Pinus, Picea abies, and Quercus in Romania; implications for their current status

    Science.gov (United States)

    Feurdean, Angelica; Tanţău, Ioan; Fărcaş, Sorina

    2011-10-01

    This paper examines fourteen fossil pollen datasets from Romania. It aims to investigate the temporal and spatial variability in the range distribution and abundance of three forest taxa, Pinus, Picea abies, and Quercus, during the Holocene. This is essential for understanding their current status in the forests of Eastern Europe, the conditions under which they arose, and the timing and processes responsible for their variability. Results from this synthesis do not indicate any apparent time lag in the establishment of Pinus diploxylon type ( Pinus sylvestris and Pinus mugo), Pinus cembra, P. abies, and Quercus across Romania within the limits of the dating resolution. However, the onset of the mass expansion of P. abies was not uniform, spreading earlier from sites in the western and north-western Carpathians (11,000-10,500 yr BP) than in the east (10,000 yr BP). We found that sites from the western, north-western, and northern Carpathians contained higher abundances of P. abies, whilst Quercus was in higher abundances in sites from the east, but there was no regional distinctiveness in the abundance of Pinus across the study area. However, P. diploxylon type was found in much higher abundance than P. cembra. Additionally, results indicate a greater proportion of Pinus (mainly P. diplxylon type) at high elevations, P. abies at mid to high elevations, and Quercus at low elevations (Pinus in the early Holocene boreal forest is likely the legacy of its local glacial refugia, fast life history strategies, high stress tolerance, and large habitat availability. In contrast, Pinus exhibited poor competitive abilities and was quickly replaced with P. abies and temperate deciduous taxa after 10,500 yr BP. P. abies has persisted in large abundances at higher elevations (above 1000 m) until the present day, as a result of good competitive abilities, and resilience to climate change and disturbance. The long-term dominance of P. abies appears to have been spatially

  11. EVALUATION OF DIFFERENT MULCH MATERIALS AND SHELTER, IN THE ESTABLISHMENT OF PLANTS OF Pinus taeda L., BY DIRECT SOWING

    Directory of Open Access Journals (Sweden)

    Marcio Ricardo Serpa

    2009-09-01

    Full Text Available This work has had as its objectives to evaluate different covering materials and physical protector's use in the forest population of Pinus taeda L. in direct sowing in the field. Three materials were used: vermiculit, dried and pricked pine needles, peel of rice and reference without covering, with and without physical protector (plastic cup of 300 ml, without botton, with five replications. The sowing was accomplished in the first half of May of 1997, being used three seeds per point. The evaluations done were: emergency at the 60 and 90 days, survival at the 120, 180 and 210 days and population density at the 210 days after sowing. The variance and averages analysis (Duncan 5%, has allowed to conclude that: the vermiculit and pine needles affected beneficially the number of plants emerged in the first 60 days, that is the most critical phase for the future of the forest population of Pinus taeda; the physical protector, provides the formation of a microenvironment, that guarantees larger percentage in the emergence, survival and initial density of plants of Pinus taeda; the direct sowing demonstrated to be a viable technique.

  12. Evidence for potential impacts of ozone on Pinus cembra L. at mountain sites in Europe: An overview

    Energy Technology Data Exchange (ETDEWEB)

    Wieser, G. [Division Alpine Timberline Ecophysiology, Bundesamt und Forschungszentrum fuer Wald, Rennweg 1, 6020 Innsbruck (Austria)]. E-mail: gerhard.wieser@uibk.ac.at; Manning, W.J. [Department of Microbiology, University of Massachusetts, Amsherst, MA 01003 (United States); Tausz, M. [Institute of Plant Sciences, Karl-Franzens Universitaet Graz, Schubertstrasse 51, 8010 Graz (Austria); School of Forest and Ecosystem Science, University of Melbourne, Water Street, Creswick Victoria 3363 (Australia); Bytnerowicz, A. [USDA Forest Service, Pacific Southwest Research Station, Riverside (United States)

    2006-01-15

    We summarize what is known about the impact of ozone (O{sub 3}) on Pinus cembra in the timberline ecotone of the central European Alps and the Carpathian Mountains. In the central European Alps exposure to ambient and two-fold ambient O{sub 3} throughout one growing season did neither cause any visible injury nor affect the photosynthetic machinery and biochemical parameters in current to 1-year-old needles. By contrast, in the southern French Alps and in the Carpathians 1-year-old needles of Pinus cembra trees showed visual symptoms similar to those observed in O{sub 3} stressed pine stands in southern California. For the southern French Alps the observed symptoms could clearly be attributed O{sub 3} and differences in O{sub 3} uptake seems to be the likely key factor for explaining the observed decline. For the Carpathians however, other reasons such as drought may not be excluded in eliciting the observed symptoms. Thus, the action of O{sub 3} has always to be evaluated in concert with other environmental impacts, determining the tree's sensitivity to stress. - An overview of ozone effects on Pinus cembra an important European coniferous tree species in the timberline ecotone.

  13. First record of the Kuwana pine mealybug Crisicoccus pini (Kuwana) in Italy: a new threat to Italian pine forests?

    Science.gov (United States)

    Boselli, Mauro; Pellizzari, Giuseppina

    2016-02-19

    The Asiatic Kuwana pine mealybug, Crisicoccus pini (Kuwana, 1902) (Hemiptera, Pseudococcidae), is reported in Italy for the first time. It was detected in September 2015 on maritime pine, Pinus pinaster, and stone pine, Pinus pinea, trees growing in the town of Cervia (Ravenna Province), Northern Italy. The mealybug has caused yellowing and decline of the pine trees. Pinus pinea is recorded here as a new host for C. pini.

  14. Geocronologia e aspectos estruturais e petrológicos do Pluton Bravo, Domínio Central da Província Borborema, Nordeste do Brasil: um granito transalcalino precoce no estágio pós-colisional da Orogênese Brasiliana

    Directory of Open Access Journals (Sweden)

    Geysson de Almeida Lages

    Full Text Available RESUMO: O Pluton Bravo (no Estado da Paraíba constitui um stock elipsoidal formado por monzo/sienogranitos porfiríticos, enclaves dioritos e zonas híbridas. Está intrudido em gnaisses migmatíticos paleoproterozoicos do Domínio Central da Província Borborema. Os sienogranitos são metaluminosos a levemente peraluminosos, e exibem altas razões de K2O/Na2O > 1,5 e FeOt/(FeOt + MgO > 0,86. Os dioritos possuem alto conteúdo de Zr (> 1.134 ppm, TiO2 ~ 1,6% e Nb > (49 ppm. As razões (LaN/YbNN estão entre 14 e 19,4, e (Eu/Eu*N, entre 0,31 e 0,37. As rochas do Pluton Bravo são moderadamente fracionadas com picos em La, Zr e forte depressão em P, Ti e menor em Sr. Os dados plotam no campo discriminante de granitos pós-tectônicos/intraplaca. As estimativas de pressão (4,4 a 6,0 Kbar baseadas no conteúdo de Alt em anfibólio sugerem posicionamento do pluton na crosta superior a média. A temperatura do liquidus de acordo com o conteúdo de Zr e SiO2 oscilou entre 847 e 893°C, e a de cristalização, calculada pelo par anfibólio-plagioclásio, entre 581 e 785°C. Exibem idades-modelo TDM = 2,35 a 2,18 e εNd (580 Ma = -18,32 a -17,03. A idade U-Pb (LA-MC-ICP-MS U-Th-Pb em zircão indica cristalização ao redor de 581 ± 2 Ma. A relação entre a idade de cristalização (~ 580 Ma, as características químicas de granito tipo-A, idades-modelo Sm-Nd maiores que 2,1 Ga e a associação com regime tectônico transcorrente contrastam com outros granitos similares, porém ligeiramente mais novos (~ 570 Ma, que ocorrem nos Domínios Central, Rio Grande do Norte e no leste da Nigéria. Isso sugere que a transição do regime compressional para direcional/componente extensional precedeu no caso deste corpo evidenciando o caráter episódico e diacrônico da Orogênese Brasiliana. Conclui-se que o Pluton Bravo constitui um bom exemplo de magmatismo pós-colisional transalcalino no Domínio Central, que, com outros exemplos no Domínio Rio Grande

  15. Influência do manejo da palhada de capim-braquiária (Brachiaria decumbens sobre o desenvolvimento inicial de soja (Glycine max e amendoim-bravo (Euphorbia heterophylla Influence of straw management of Brachiaria decumbens on the initial development of Glycine max and Euphorbia heterophylla

    Directory of Open Access Journals (Sweden)

    C.D.G. Maciel

    2003-12-01

    Full Text Available Com o objetivo de avaliar os efeitos de diferentes manejos de palhada de capim-braquiária sobre o desenvolvimento inicial da cultura de soja e da planta daninha amendoimbravo, foi conduzido um experimento em condições de casa de vegetação no NuPAMFCA/UNESP, BotucatuSP. Os tratamentos utilizados foram: manejo da palhada na superfície do solo + irrigação superficial (T1; manejo da palhada na superfície do solo + irrigação subsuperficial (T2; palhada incorporada ao solo (T3; e testemunha sem cobertura (T4. A palhada foi colhida no campo 30 dias após dessecação com o herbicida glyphosate (1,44 g i.a. ha-1. O delineamento experimental utilizado foi o inteiramente casualizado, com sete repetições, sendo as unidades experimentais vasos plásticos, com a soja e o amendoim-bravo semeados paralelamente, em linhas distintas. O T1 reduziu significativamente o índice de velocidade de germinação (IVG e a altura das plântulas de soja aos 5 e 10 dias após a emergência (DAE, ao contrário do amendoim-bravo, o qual não sofreu interferência dos tratamentos estudados, constituindo-se em uma planta-problema para sistemas produtivos com palhada de capim-braquiária. Os resultados da análise de crescimento (TCA - taxa de crescimento absoluto, TCR - taxa de crescimento relativo e TAL - taxa de assimilação líquida das plântulas de soja e amendoim-bravo apresentaram valores máximos aos 15 DAE, com exceção do T3 para soja, o qual reduziu expressivamente o desenvolvimento em relação aos demais tratamentos.An experiment was carried out under greenhouse conditions at NuPAM-FCA/UNESP, Botucatu, SP, Brazil to evaluate the effects of different types of straw management of Brachiaria decumbens on the initial development of Glycine max and Euphorbia heterophylla. The treatments were: straw on soil surface + irrigation on the top (T1; straw on soil surface + underirrigation (T2; straw incorporated into the soil (T3 and control (T4. B. decumbens

  16. COMBUSTION CHARACTERISTICS OF TORREFIED WOOD SAMPLES OF PINUS CARREBEA AND LEUCAENA LEUCOCEPHALA GROWN IN NIGERIA

    Directory of Open Access Journals (Sweden)

    Joseph Adeola FUWAPE

    2016-12-01

    Full Text Available Torrefaction of selected wood samples of Pinus Carrebea and Leucaena Leucocephala were carried out at temperatures ranging from 200 to 300°C to improve the energy parameters of biomass and to determine the effect of torrefication temperature on the physical and combustion properties of wood selected from Pinus carrebea and Leuceanea leucocephala grown in Nigeria. In this process the biomass hemicellulose is degraded, maintaining its cellulose and lignin content. The samples were dried and heated to 225, 250, 275, and 300°C. Then the torrefied mass was subjected to basic property testing on proximate analysis and heating value was calculated in order to understand the differences between raw material and its torrefied products. Specifically, the wood blocks changed from light brown to black, stemming from the partial carbonization at the wood surface. When the temperature is 225°C, the color of the wood is between dark brown and once the torrefaction temperatures are 250 and 275°C, the colors of the wood become dark and darker respectively. The results of the proximate analysis also showed that increasing of torrefied temperature; volatile fraction was reduced while fixed carbon was increased with increase in temperature from 21.34 to 52.74 and 18.58 to 56.83 for Leucaena leucocephala and Pinus carreabeanus respectively at 225 to 300°C. The volatile content is decreased from 78.58% to 62.76% with increase in temperature. Ash content of were within 1.57-3.41% of torrefied wood. It could be observed that the High calorific value (HCV for pine ranged between 19.80 and 28.06MJ/Kg for the top, 19.93and 24.96MJ/kg for middle with 19.72and 25.96MJ/Kg for base. The values recorded for raw sample and at 275°C been the lowest and highest respectively. The High calorific value (HCV were found to be on the increase and nose dive at 300°C for the tree parts used in this research. The result revealed that for Leuceana the value increased from raw up to

  17. Essential oil composition variability among natural populations of Pinus mugo Turra in Kosovo.

    Science.gov (United States)

    Hajdari, Avni; Mustafa, Behxhet; Ahmeti, Gresa; Pulaj, Bledar; Lukas, Brigitte; Ibraliu, Alban; Stefkov, Gjoshe; Quave, Cassandra L; Novak, Johannes

    2015-01-01

    Pinus mugo Turra, is a native pine species in central and southern Europe, growing in high mountains area (altitudes 1.800-2.300 m.a.s.l.). In Kosovo, it is one of the native pines too, distributed in high altitudes in the Sharri Mountains and Albanian Alps Mountains. Its populations represent an important wealth of essential oil resources available, which make this species very important in terms of economic values. The chemical composition and yields of the essential oils of dwarf pine (Pinus mugo Turra) needles, twigs and cones from six wild populations in Kosovo were investigated with the aim to assess their natural variability. The identity of P. mugo was confirmed by morphology and DNA barcoding. Sixty-two compounds were identified representing 69-95 % of the total identified compounds. The yield ranged from 0.3-0.8 % v/w in needles, 1.0-2.4 % v/w in twigs and 0.1-0.5 % v/w in cones, depending on the origin of plant material and plant organs. α-Pinene (needles: 16.9-24.5 %; twigs: 4.5-8.8 %; cones: 3.1-5.6 %), β-pinene (needles: 1.5-5.4 %; twigs: 2.2-15.4 %; cones: 1.3-14.2 %), δ-3-carene (needles: 15.4-27.8 %; twigs: 24.0-51.6 %; cones: 10.5-31.5 %), limonene + β-phellandrene (needles: 1.9-5.9 %; twigs: 12.6-24.2 %; cones: 2.1-9.3 %), (E)-caryophyllene (needles: 4.4-8.9 %; twigs: 4.0-10.8 %; cones: 10.3-26.9 %) and germacrene D (needles: 4.0-8.3 %; twigs: 0.2-6.19 %; cones: 0.1-12.4 %) were the major components of the essential oil. Principal component analysis (PCA) and hierarchical cluster analyses (HCA) suggests that the population of P. mugo clustering is not related to their geographic location, but rather seemed to be linked to local selective forces acting on chemotype diversity. Low variability related to their geographic location has an economic importance since samples originating from different locations in Kosovo can treated with same standards.

  18. Fauna do solo em áreas com Eucalyptus spp. e Pinus elliottii, Santa Maria, RS.

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    Maria Izabel Jacques Soares

    2009-10-01

    Full Text Available Normal 0 21 MicrosoftInternetExplorer4 /* Style Definitions */ table.MsoNormalTable {mso-style-name:"Tabela normal"; mso-tstyle-rowband-size:0; mso-tstyle-colband-size:0; mso-style-noshow:yes; mso-style-parent:""; mso-padding-alt:0cm 5.4pt 0cm 5.4pt; mso-para-margin:0cm; mso-para-margin-bottom:.0001pt; mso-pagination:widow-orphan; font-size:10.0pt; font-family:"Times New Roman";} Este trabalho, com início na primavera de 1996 e término no inverno de 1997, teve como objetivo realizar o levantamento populacional da fauna do solo em áreas com Eucalyptus spp. e Pinus elliottii, situadas no Campus da Universidade Federal de Santa Maria, Rio Grande do Sul, Brasil. Para a coleta do solo, utilizou-se um extrator, formando-se amostras em dez pontos, nas profundidades de 0-5, 5-10, 10-15 e de 15-20 cm, em cada estação do ano. Por meio deste estudo, pôde-se identificar alguns representantes dos Phylum Annelida e Arthropoda que participam do contexto fauna-solo-vegetação. O total de indivíduos, seu respectivo percentual e número de indivíduos por metro quadrado foram obtidos. Observou-se o predomínio do Phylum Arthropoda e, mais especificamente, da classe Insecta na área com Eucalyptus spp. e do Phylum Annelida na área com Pinus elliottii. A variável profundidade originou modelos matemáticos que explicam a distribuição populacional dentre as áreas estudadas. A variável estação do ano não apresentou uma diferença significativa. Não foi possível, portanto, estabelecer um modelo de distribuição populacional.

  19. Radiocarbon dating of a pine tree (Pinus densiflora) from Yeongwol, Korea

    Energy Technology Data Exchange (ETDEWEB)

    Kim, C.H.; Lee, J.H.; Kang, J.; Song, S.; Yun, M.H. [AMS Lab., NCIRF, Seoul National University, Seoul 151-742 (Korea, Republic of); Kim, J.C. [Dept. of Physics and Astronomy, Seoul National University, Seoul 151-742 (Korea, Republic of)

    2015-10-15

    We report the results of the dating of a pine tree (Pinus densiflora) from Yeongwol, Korea. The age of the tree was estimated to be in the range of hundreds of years, however, the tree had been broken by a strong wind in March 2010 and now only the stump of the tree is left. At the time of sampling in 2014, there were several decayed parts in the stump, so using the usual dendrochronological method (i.e. ring counting) for dating was difficult. However, we found a small wood sample with tree rings near the center of the stump that could be used for radiocarbon wiggle-match dating. Radiocarbon dates were determined using Accelerator mass spectrometry (AMS). The IntCal13 curve was used to calibrate the radiocarbon dates, and the wiggle matching technique was used to reduce the error of the calibrated ages. Based on the dating results, we suggest that the pine tree is approximately 300 years or older.

  20. Pharmacophore Modeling and Molecular Docking Studies on Pinus roxburghii as a Target for Diabetes Mellitus

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    Pawan Kaushik

    2014-01-01

    Full Text Available The present study attempts to establish a relationship between ethnopharmacological claims and bioactive constituents present in Pinus roxburghii against all possible targets for diabetes through molecular docking and to develop a pharmacophore model for the active target. The process of molecular docking involves study of different bonding modes of one ligand with active cavities of target receptors protein tyrosine phosphatase 1-beta (PTP-1β, dipeptidyl peptidase-IV (DPP-IV, aldose reductase (AR, and insulin receptor (IR with help of docking software Molegro virtual docker (MVD. From the results of docking score values on different receptors for antidiabetic activity, it is observed that constituents, namely, secoisoresinol, pinoresinol, and cedeodarin, showed the best docking results on almost all the receptors, while the most significant results were observed on AR. Then, LigandScout was applied to develop a pharmacophore model for active target. LigandScout revealed that 2 hydrogen bond donors pointing towards Tyr 48 and His 110 are a major requirement of the pharmacophore generated. In our molecular docking studies, the active constituent, secoisoresinol, has also shown hydrogen bonding with His 110 residue which is a part of the pharmacophore. The docking results have given better insights into the development of better aldose reductase inhibitor so as to treat diabetes related secondary complications.

  1. The role of endophytic fungal individuals and communities in the decomposition of Pinus massoniana needle litter.

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    Zhilin Yuan

    Full Text Available The role of fungal endophytes (FEs as "pioneer" decomposers has recently been recognized; however, the extent to which FEs contribute to litter loss is less well understood. The genetic and enzymatic bases of FE-mediated decomposition have also rarely been addressed. The effects of populations and individuals (with an emphasis on two dominant Lophodermium taxa of FEs on needle-litter decomposition were assessed for Pinus massoniana, a ubiquitous pine in southern China. Data from in vivo (microcosm experiments indicated that the percentage of litter-mass loss triggered by FEs was linearly correlated with incubation time and approached 60% after seven months. In vitro decomposition tests also confirmed that endophytic Lophodermium isolates caused 14-22% mass loss within two months. Qualitative analysis of exoenzymes (cellulase and laccase, important for lignocellulose degradation revealed that almost all of the Lophodermium isolates showed moderate or strong positive reactions. Furthermore, partial sequences of β-glucosidase (glycoside hydrolase family 3, GH3, laccase, and cellobiohydrolase (GH7 genes were amplified from Lophodermium isolates as "functional markers" to evaluate their potential for lignocellulolytic activity. Three different genes were detected, suggesting a flexible and delicate decomposition system rich in FEs. Our work highlights the possibility that the saprophytism and endophytism of FEs may be prerequisites to initiating rapid decomposition and thus may be key in Fes' contribution to litter decomposition, at least in the early stage. Potential indicators of the presence of core fungal decomposers are also briefly discussed.

  2. Modeling compatible single-tree aboveground biomass equations for masson pine (Pinus massoniana) in southern China

    Institute of Scientific and Technical Information of China (English)

    ZENG Wei-sheng; TANG Shou-zheng

    2012-01-01

    Because of global climate change,it is necessary to add forest biomass estimation to national forest resource monitoring.The biomass equations developed for forest biomass estimation should be compatible with volume equations.Based on the tree volume and aboveground biomass data of Masson pine (Pinus massoniana Lamb.) in southern China,we constructed one-,two-and three-variable aboveground biomass equations and biomass conversion functions compatible with tree volume equations by using error-in-variable simultaneous equations.The prediction precision of aboveground biomass estimates from one variable equation exceeded 95%.The regressions of aboveground biomass equations were improved slightly when tree height and crown width were used together with diameter on breast height,although the contributions to regressions were statistically insignificant.For the biomass conversion function on one variable,the conversion factor decreased with increasing diameter,but for the conversion function on two variables,the conversion factor increased with increasing diameter but decreased with increasing tree height.

  3. Essential Oil Composition of Pinus peuce Griseb. Needles and Twigs from Two National Parks of Kosovo

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    Avni Hajdari

    2016-01-01

    Full Text Available The principal aim of this study was to analyze the chemical composition and qualitative and quantitative variability of essential oils obtained from seven naturally grown populations of the Pinus peuce Grisebach, Pinaceae in Kosovo. Plant materials were collected from three populations in the Sharri National Park and from four other populations in the Bjeshkët e Nemuna National Park, in Kosovo. Essential oils were obtained by steam distillation and analyzed by GC-FID (Gas Chromatography-Flame Ionization Detection and GC-MS (Gas Chromatography-Mass Spectrometry. The results showed that the yield of essential oils (v/w dry weight varied depending on the origin of population and the plant organs and ranged from 0.7 to 3.3%. In total, 51 compounds were identified. The main compounds were α-pinene (needles: 21.6–34.9%; twigs: 11.0–24%, β-phellandrene (needles: 4.1–27.7; twigs: 29.0–49.8%, and β-pinene (needles: 10.0–16.1; twigs: 6.9–20.7%. HCA (Hierarchical Cluster Analysis and PCA (Principal Component Analyses were used to assess geographical variations in essential oil composition. Statistical analysis showed that the analyzed populations are grouped in three main clusters which seem to reflect microclimatic conditions on the chemical composition of the essential oils.

  4. Essential Oil Composition of Pinus peuce Griseb. Needles and Twigs from Two National Parks of Kosovo

    Science.gov (United States)

    Hajdari, Avni; Mustafa, Behxhet; Selimi, Hyrmete; Veselaj, Zeqir; Breznica, Pranvera; Novak, Johannes

    2016-01-01

    The principal aim of this study was to analyze the chemical composition and qualitative and quantitative variability of essential oils obtained from seven naturally grown populations of the Pinus peuce Grisebach, Pinaceae in Kosovo. Plant materials were collected from three populations in the Sharri National Park and from four other populations in the Bjeshkët e Nemuna National Park, in Kosovo. Essential oils were obtained by steam distillation and analyzed by GC-FID (Gas Chromatography-Flame Ionization Detection) and GC-MS (Gas Chromatography-Mass Spectrometry). The results showed that the yield of essential oils (v/w dry weight) varied depending on the origin of population and the plant organs and ranged from 0.7 to 3.3%. In total, 51 compounds were identified. The main compounds were α-pinene (needles: 21.6–34.9%; twigs: 11.0–24%), β-phellandrene (needles: 4.1–27.7; twigs: 29.0–49.8%), and β-pinene (needles: 10.0–16.1; twigs: 6.9–20.7%). HCA (Hierarchical Cluster Analysis) and PCA (Principal Component Analyses) were used to assess geographical variations in essential oil composition. Statistical analysis showed that the analyzed populations are grouped in three main clusters which seem to reflect microclimatic conditions on the chemical composition of the essential oils. PMID:27579344

  5. Increased selfing and correlated paternity in a small population of a predominantly outcrossing conifer, Pinus sylvestris.

    Science.gov (United States)

    Robledo-Arnuncio, J J; Alía, R; Gil, L

    2004-09-01

    Outcrossing rate, the rates of ovule and seed abortion, and levels of correlated paternity were estimated in a small population of Pinus sylvestris, a predominantly outcrossing conifer, and were compared with estimates from two widely dispersed woodlands of the same species, showing a range of densities. On average, seed trees of the small population showed an eight-fold higher selfing rate (25 vs. 3%) and a 100-fold greater incidence of correlated paternity (19.6 vs. 0.2%) than did trees from the large populations. No evidence was found of pollen limitation within the remnant stand, as suggested by ovule abortion rates. Investigation of the mating patterns in the small population, based on the unambiguous genealogy of 778 open-pollinated seeds, showed a large departure from random mating. Only 8% of the possible mating pairs within the stand were observed. Correlated paternity rate within a maternal sibship was negatively associated (rs = -0.398, P shared paternity among maternal sibships was negatively correlated (rs = -0.704, P < 0.001) with the distance between seed trees. Numerical simulations, based on the estimated individual pollen dispersal kernel, suggest that restricted dispersal might have been the key factor affecting mating patterns in the small population and, together with low population density, may account for the observed mating system variation between the small and the large populations. The results of this study show that a severe size reduction may substantially affect the mating system of a wind-pollinated, typically outcrossed plant species.

  6. Genet Variation of Ectomycorrhizal Suillus granulatus Fruiting Bodies in Pinus strobus Stands.

    Science.gov (United States)

    Lee, Hwa-Yong; Koo, Chang-Duck

    2016-03-01

    The genets of Suillus granulatus in a Pinus strobus stand (13 m × 60 m) were identified using random amplified polymorphic DNA molecular markers and the DNA of mushrooms that fruited for two years, and variations in genet size and distribution were analyzed. From a total of 116 mushrooms, 73 genets were identified and were grouped into three locations. The genets of mushrooms in close proximity differed from each other. The genet sizes varied at any of the three locations. The lengths of the identified genets in the pine stand ranged from 0.09 to 2.90 m. The average number of mushrooms per genet was 1.2 to 2.3, and the percentage of genets that were represented by a single mushroom was 44% to 94%. This variation in the genets of mushrooms in close proximity suggests that the ectomycorrhizal mycelial bodies of S. granulatus propagated sexually by fusing haploid spores derived from the mushrooms gills with below-ground mycelia. Therefore, it is necessary further to investigate the formation of new genets through spores in ectomycorrhizal fungal colonies.

  7. Photosynthetic induction responses of Pinus koraiensis seedlings grown in different light environments

    Institute of Scientific and Technical Information of China (English)

    ZHOUYong-bin; YINYou; LIUXing-shuang; WANGQing-li

    2004-01-01

    The time processes of photosynthetic induction responses to various irradiances in Korean pine (Pinus koraiensis) seedlings grown in open-light environments and in understory of forest were studied in an area near the Research Station of Changbai Mountain Forest Ecosystems, Jilin Province, China from July 15 to August 5, 1997. The results showed that at 200 IJmol·m-2·s-1 photosynthetic photon flux density (PPFD) and 500 pmol·m-2·s-1 PPFD, the induction time for the photosynthetic rates of understory-grown seedlings to reach 50% and 90% steady-state net photosynthetic rates was longer than that of the open-grown seedlings. The induction responses of open-growth seedlings at 500 pmol·m-2·s-1 PPFD were slower than those at 200 pmol·m-2·s-1 PPFD, but it was the very reverse for understory-growth seedlings, which indicates that the photosynthetic induction times of Korean pine seedlings grown in the understory depended on the sunfleck intensity.

  8. Spatial variability of throughfall in a Chinese Pine (Pinus tabulaeformis) plantation in northern China

    Institute of Scientific and Technical Information of China (English)

    ZHAN Weiqing; ZHANG Zhiqiang; WU Jun; XIAO Jinqiang

    2007-01-01

    The interception of rainfall by vegetation and the subsequent evaporation of intercepted water from the canopy surface play an important role in hydrological processes,and the water and energy balance of forest ecosystems.Spatial variability of interception has different effects on water yield from watersheds located in different climatic and biome regions.In order to explain the spatial patterns of interception,we adopted grid-sampling method to install rain-gauges to measure throughfall.Results show that the coefficient of variation (Cv) of throughfall tends to decline as rain intensity increases.After the canopy is saturated,Cv of throughfall remained at a constant value,which is close to the Cv of the canopy leaf area index (LAI) value 0.18.Thus,the Cv of LAI is regarded as the extremum of that of throughfall.Because of the special characteristic of Chinese pine (Pinus tabulaeformis),and the lower droopy branches,negative values for interception account for only 13% of the total samples.Furthermore,the max is above 70% of gross rainfall.

  9. Observation and modeling of NPP for Pinus elliottii plantation in subtropical China

    Institute of Scientific and Technical Information of China (English)

    MA ZeQing; LIU QiJing; WANG HuiMin; LI XuanRan; ZENG HuiQing; XU WenJia

    2008-01-01

    Based on the stem analysis of 59 individuals of Pinus elliottii in combination with tree biomass models,we calculated annual biomass increment of forest plots at Qianyanzhou Ecological Station,Chinese Academy of Sciences in subtropical China. In addition,canopy layer and community NPP were calculated based on 12 years' litter fall data. NPP of the 21-year-old forest was estimated by using the BIOME BGC model; and both measured NPP and estimated NPP were compared with flux data. Community lation between annual litter fall and annual biomass increment; and the litter fall was 1.19 times the biomass increment of living trees. From 1985 to 2005,average NPP and GPP values based on BGC simulated tree layer NPP values. NPP accounted for 30.2% (25.6%-32.9%) of GPP,while NEP accounted for 57.5% (48.1%-66.5%) of tree-layer NPP and 41.74% (37%-52%) of stand NPP. Soil respiration accounted for 77.0% of measured tree NPP and 55.9% of the measured stand NPP. NEE based on eddy covariance method was 12.97% higher than the observed NEP.

  10. Horse grazing systems: understory biomass and plant biodiversity of a Pinus radiata stand

    Directory of Open Access Journals (Sweden)

    Antonio Rigueiro-Rodríguez

    2012-02-01

    Full Text Available Horse grazing systems may affect productivity and biodiversity of understory developed under Pinus radiata D. Don silvopastoral systems, while acting as a tool to reduce the risk of fire. This study compared continuous and rotational grazing systems effect upon biomass, fractions of stem, sprouts, leaves and woody parts of Ulex europaeus L. and alpha (Species Richness, Shannon-Wiener and beta (Jaccard and Magurran biodiversity for a period of four years in a P. radiata silvopastoral system. The experiment consisted of a randomized block design of two treatments (continuous and rotational grazing. Biomass, and species abundances were measured - biodiversity metrics were calculated based on these results for a two years of grazing and two years of post-grazing periods. Both continuous and rotational grazing systems were useful tools for reducing biomass and, therefore, fire risk. The rotational grazing system caused damage to the U. europaeus shrub, limiting its recovery once grazing was stopped. However, the more intensive grazing of U. europaeus plants under rotational had a positive effect on both alpha and beta biodiversity indexes due to the low capacity of food selection in the whole plot rather than continuous grazing systems. Biomass was not affected by the grazing system; however the rotational grazing system is more appropriate to reduce U. europaeus biomass and therefore forest fire risk at a long term and to enhance pasture biodiversity than the continuous grazing system.

  11. Response of Pinus sylvestris roots to sheet-erosion exposure: an anatomical approach

    Science.gov (United States)

    Rubiales, J. M.; Bodoque, J. M.; Ballesteros, J. A.; Diez-Herrero, A.

    2008-03-01

    Anatomical changes of exposed tree roots are valuable tools to date erosion events, but the responses of diverse species under different types of erosion need still to be studied in detail. In this paper we analyze the histological changes that occur in roots of Scots pine (Pinus sylvestris L.) subjected to continuous denudation. A descriptive and quantitative study was conducted in the Senda Schmidt, a popular trail located on the northern slope of the Sierra de Guadarrama (Central Iberian System, Spain). Measurement of significant parameters allowed the moment of exposure of the roots to be identified. These parameters were: a) width of the growth ring; b) number of cells per ring; c) percentage of latewood and d) diameter of cellular light in earlywood. A one-way analysis ANOVA was also carried out in order to establish statistically significant differences between homogeneous groups of measurements in pre-exposed and exposed roots. Based on these analyses, Scots pine roots show a remarkable anatomical response to sheet-erosion exposure. Increased growth in the ring is accompanied by a slight reduction of the cell lumina of the earlywood tracheids. At the end of the ring, several rows of thick-walled tracheids define latewood tissue and visible annual borders very clearly. Furthermore, resin ducts often appear in tangential rows, increasing resin density in the tissue. All of these indicators made it possible to determine with precision the first year of exposure and to estimate precisely sheet erosion rates.

  12. Pinus sylvestris as a missing source of nitrous oxide and methane in boreal forest.

    Science.gov (United States)

    Machacova, Katerina; Bäck, Jaana; Vanhatalo, Anni; Halmeenmäki, Elisa; Kolari, Pasi; Mammarella, Ivan; Pumpanen, Jukka; Acosta, Manuel; Urban, Otmar; Pihlatie, Mari

    2016-01-01

    Boreal forests comprise 73% of the world's coniferous forests. Based on forest floor measurements, they have been considered a significant natural sink of methane (CH4) and a natural source of nitrous oxide (N2O), both of which are important greenhouse gases. However, the role of trees, especially conifers, in ecosystem N2O and CH4 exchange is only poorly understood. We show for the first time that mature Scots pine (Pinus sylvestris L.) trees consistently emit N2O and CH4 from both stems and shoots. The shoot fluxes of N2O and CH4 exceeded the stem flux rates by 16 and 41 times, respectively. Moreover, higher stem N2O and CH4 fluxes were observed from wet than from dry areas of the forest. The N2O release from boreal pine forests may thus be underestimated and the uptake of CH4 may be overestimated when ecosystem flux calculations are based solely on forest floor measurements. The contribution of pine trees to the N2O and CH4 exchange of the boreal pine forest seems to increase considerably under high soil water content, thus highlighting the urgent need to include tree-emissions in greenhouse gas emission inventories.

  13. Integrated physiological and hormonal profile of heat-induced thermotolerance in Pinus radiata.

    Science.gov (United States)

    Escandón, Mónica; Cañal, María Jesús; Pascual, Jesús; Pinto, Glória; Correia, Barbara; Amaral, Joana; Meijón, Mónica

    2016-01-01

    Despite great interest, not only from the economic point of view but also in terms of basic science, research on heat stress tolerance in conifers remains scarce. To fill this gap, a time-course experiment using expected temperature increase was performed aiming to identify physiological and biochemical traits that allow the characterization of heat-induced thermotolerance and recovery in Pinus radiata D. Don plants. Several physiological parameters were assessed during heat exposure and after recovery, and multiple phytohormones-abscisic acid (ABA), indole-3-acetic acid (IAA), cytokinins (CKs), gibberellins, jasmonic acid, salicylic acid (SA) and brassinosteroids-were quantified by ultra-performance liquid chromatography-mass spectrometry from unique sample. Furthermore, tissue specific stress-signaling was monitored by IAA and ABA immunolocalization. Multivariate statistical analysis of the data enabled clustering of the shorter- and longer-term effects of heat stress exposure. Two sequential physiological responses were identified: an immediate and a delayed response, essentially determined by specific phytohormones, proline, malondialdehyde and total soluble sugar patterns. Results showed that ABA and SA play a crucial role in the first stage of response to heat stress, probably due to the plant's urgent need to regulate stomatal closure and counteract the increase in oxidative membrane damage demonstrated in shorter-term exposures. However, in longer exposures and recovery, proline, total sugars, IAA and CKs seem to be more relevant. This integrated approach pinpointed some basic mechanisms of P. radiata physiological responses underlying thermotolerance processes and after recovery.

  14. Transpiration rates and canopy conductance of Pinus radiata growing with different pasture understories in agroforestry systems.

    Science.gov (United States)

    Miller, Blair J.; Clinton, Peter W.; Buchan, Graeme D.; Robson, A. Bruce

    1998-01-01

    We measured tree transpiration and canopy conductance in Pinus radiata D. Don at two low rainfall sites of differing soil fertility in Canterbury, New Zealand. At the more fertile Lincoln site, we also assessed the effects of two common pasture grasses on tree transpiration and canopy conductance. At the less fertile Eyrewell Forest site, the effect of no understory, and the effects of irrigation in combination with mixtures of grass or legume species were determined. Tree xylem sap flux (F(d)') was measured by the heat pulse method. Total canopy conductance to diffusion of water vapor (G(t)) was calculated by inverting a simplified Penman-Monteith model. The different treatment effects were modeled by the simple decaying exponential relationship G(t) = G(tmax)e((-bD)), where D = air saturation deficit. At the Lincoln site, trees with an understory of cocksfoot had lower F(d)' and G(tmax) than trees with an understory of ryegrass, although the sensitivity of G(t) to increasing D (i.e., the value of b) did not differ between treatments. At the Eyrewell site, irrigation only increased F(d)' in the absence of an understory, whereas the presence of understory vegetation, or lack of irrigation, or both, significantly reduced G(tmax) and increased b. We conclude that the selection of understory species is critical in designing successful agroforestry systems for low rainfall areas.

  15. THE COMBUSTION PROPERTIES OF LAMINATED WOOD MATERIALS PREPARED FROM SCOTCH PINE (Pinus sylvestris L.

    Directory of Open Access Journals (Sweden)

    Ramazan ÖZEN

    2001-01-01

    Full Text Available In this study, the combustion properties of 3 ply laminated wood material, which was produced from scotch pine (Pinus sylvestris L. impregnated with Sodium perborat, Sodium tetra borat, Imersol (I-WR 2000 and TanalithCBC (T-CBC by using dipping method has been investigated. Prepared materials have been bonded with Desmodur- VTKA adhesive and tested according to the procedure of ASTM-E 69 standards. As a result, the highest weight loss (60.83 g in laminated sample impregnated with I-WR 2000, CO rate (6340.85 ppm in neutral sample impregnated with T-CBC, CO2 rate (7.48 %, O2 rate (13.03 % and according to the first weight rate the highest combustion rate (82.73 % in control samples, heat increasing (406.55 o C in laminated sample impregnated with T-CBC have been obtained. According to these results, in the combustion tests of laminated samples sodium tetra borat and sodium perborat have been determined as a successful fire retardant chemical.

  16. Anti-pseudomonas activity of essential oil, total extract, and proanthocyanidins of Pinus eldarica Medw. bark

    Directory of Open Access Journals (Sweden)

    Masoud Sadeghi

    2016-01-01

    Full Text Available Pinus eldarica Medw. (Iranian pine is native to Transcaucasian region and has been vastly planted in Iran, Afghanistan, and Pakistan. Various parts of this plant have been widely used in traditional medicine for the treatment of various diseases including infectious conditions (e.g. infectious wounds. In this study we aimed to investigate the antibacterial activity of P. eldarica bark extract, essential oil and proanthocyanidins on three important bacteria, Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa. Antibacterial analysis was performed using standard disk diffusion method with different concentrations of essential oil, bark total hydroalcoholic extract, and bark proanthocyanidins (0.5, 1, 2 and 3 mg/ml. After incubation at 37 °C for 24 h, the antibacterial activity was assessed by measuring the zone of growth inhibition surrounding the disks. The results indicated that the essential oil, total hydroalcoholic extract, and proanthocyanidins of the bark of the P. eldarica were effective against the gram negative bacteria, P. aeruginosa, and significantly inhibited its growth in disk diffusion method (P<0.001 of which the essential oil had the most potent inhibitory effect. However, none of the bark preparations could significantly inhibit the growth of S. aureus or E. coli. Our findings showed that P. eldarica bark components have significant anti-pseudomonas activity having potentials for new sources of antibacterial agents or antibacterial herbal preparations.

  17. ENERGY BALANCE AND CO2 EXCHANGE BEHAVIOUR IN SUB-TROPICAL YOUNG PINE (Pinus roxburghii PLANTATION

    Directory of Open Access Journals (Sweden)

    B. K. Bhattacharya

    2012-08-01

    Full Text Available A study was conducted to understand the seasonal and annual energy balance behaviour of young and growing sub-tropical chir pine (Pinus roxburghii plantation of eight years age in the Doon valley, India and its coupling with CO2 exchange. The seasonal cycle of dekadal daytime latent heat fluxes mostly followed net radiation cycle with two minima and range between 50–200 Wm-2 but differed from the latter during the period when soil wetness and cloudiness were not coupled. Dekadal evaporative fraction closely followed the seasonal dryness-wetness cycle thus minimizing the effect of wind on energy partitioning as compared to diurnal variation. Daytime latent heat fluxes were found to have linear relationship with canopy net assimilation rate (Y = 0.023X + 0.171, R2 = 0.80 though nonlinearity exists between canopy latent heat flux and hourly net CO2 assimilation rate . Night-time plant respiration was found to have linear relationship (Y = 0.088 + 1.736, R2 = 0.72 with night-time average vapour pressure deficit (VPD. Daily average soil respiration was found to be non-linearly correlated to average soil temperatures (Y = -0.034X2 + 1.676X – 5.382, R2 = 0.63 The coupled use of empirical models, seasonal energy fluxes and associated parameters would be useful to annual water and carbon accounting in subtropical pine ecosystem of India in the absence high-response eddy covariance tower.

  18. Larvicidal efficacies and chemical composition of essential oils of Pinus sylvestris and Syzygium aromaticum against mosquitoes

    Institute of Scientific and Technical Information of China (English)

    Kehinde Adenike Fayemiwo; Monsuru Adebayo Adeleke; Ovie Princewill Okoro; Shola Hezekiah Awojide; Ilias Olufemi Awoniyi

    2014-01-01

    Objective:To assess the chemical composition and mosquito larvicidal potentials of essential oils of locally sourced Pinus sylvestris (P. sylvestris) and Syzygium aromaticum (S. aromaticum) against Aedes aegypti (A. aegypti) and Culex quinquefasciatus (C. quinquefasciatus). Method:The chemical composition of the essential oils of both plants was determined using GC-MS while the larvicidal bioassay was carried out using different concentrations of the oils against the larvae of A. aegypti and C. quinquefasciatus in accordance with the standard protocol. Results:The results as determined by GC-MS showed that oil of S. aromaticum has eugenol (80.5%) as its principal constituent while P. sylvestris has 3-Cyclohexene-1-methanol, .alpha., .alpha.4-trimethyl (27.1%) as its dominant constituent. Both oils achieved over 85%larval mortality within 24 h. The larvae of A. aegypti were more susceptible to the oils [LC50 (S. aromaticum)=92.56 mg/L, LC50(P. sylvestris)=100.39 mg/L] than C. quinquefasciatus [LC50(S. aromaticum)=124.42 mg/L;LC50(P. sylvestris)=128.00 mg/L]. S. aromaticum oil was more toxic to the mosquito larvae than oil of P. sylvestris but the difference in lethal concentrations was insignificant (P>0.05). Conclusion: The results justify the larvicidal potentials of both essential oils and the need to incorporate them in vector management and control.

  19. Association between herbivore stress and glutathione S-transferase expression in Pinus brutia Ten.

    Science.gov (United States)

    Semiz, A; Çelik-Turgut, G; Semiz, G; Özgün, Ö; Şen, A

    2016-03-31

    Plants have developed mechanisms to defend themselves against many factors including biotic stress such as herbivores and pathogens. Glutathione S-transferase (GST) is a glutathione-dependent detoxifying enzyme and plays critical roles in stress tolerance and detoxification metabolism in plants. Pinus brutia Ten. is a prominent native forest tree species in Turkey, due to both its economic and ecological assets. One of the problems faced by P. brutia afforestation sites is the attacks by pine processionary moth (Thaumetopoea wilkinsoni Tams.). In this study, we investigated the changes in activity and mRNA expression of GST in pine samples taken from both resistant and susceptible clones against T. wilkinsoni over a nine month period in a clonal seed orchard. It was found that the average cytosolic GST activities of trees in March and July were significantly higher than the values obtained in November. November was considered to be the control since trees were not under stress yet. In addition, RT-PCR results clearly showed that levels of GST transcripts in March and July samples were significantly higher as compared to the level seen in November. These findings strongly suggest that GST activity from P. brutia would be a valuable marker for exposure to herbivory stress.

  20. Comparative Analysis of Plant Diversity of Pinus tabulaeformis Forests in Ten Regions of Beijing Mountainous Areas

    Institute of Scientific and Technical Information of China (English)

    Li Liping; Xing Shaohua; Zhao Bo; Wang Qingchun; Cui Guofa

    2006-01-01

    Based on investigations during 1998-2003,shrubs and herbs,as well as indicators of similarity and diversity in Pinus tabulaeformis forests of ten regions around Beijing were compared and analyzed,and protection measures were suggested.Generally,the shrubs and herbs in P.tabulaeformis forests of Shidu,Mutianyu,and Yunfengshan are rich in species diversity and have great similarities.The percentages of common species in each of these three paired regions are above 50%,while many peculiar plant species that could not be found in the other nine regions exist in Labagoumen.As for plant diversity indices,plants in the P.tabulaeformis forests of Baihuashan,Shidu,and Labagoumen occur more frequently than in other regions,while the number of plants in Tanjiesi and Miaofengshan are far less.Some endangered orchid species were found distributed in P.tabulaeformis forests with a clumped spatial pattern but rarely appeared in other forest types,indicating that P.tabulaeformis forests are ideal habitats for orchid species.

  1. Ostryopsis davidiana seedlings inoculated with ectomycorrhizal fungi facilitate formation of mycorrhizae on Pinus tabulaeformis seedlings.

    Science.gov (United States)

    Bai, Shu-Lan; Li, Guo-Lei; Liu, Yong; Kasten Dumroese, R; Lv, Rui-Heng

    2009-08-01

    Reforestation in China is important for reversing anthropogenic activities that degrade the environment. Pinus tabulaeformis is desired for these activities, but survival and growth of seedlings can be hampered by lack of ectomycorrhizae. When outplanted in association with Ostryopsis davidiana plants on reforestation sites, P. tabulaeformis seedlings become mycorrhizal and survival and growth are enhanced; without O. davidiana, pines often remain without mycorrhizae and performance is poorer. To better understand this relationship, we initiated an experiment using rhizoboxes that restricted root and tested the hypothesis that O. davidiana seedlings facilitated ectomycorrhizae formation on P. tabulaeformis seedlings through hyphal contact. We found that without O. davidiana seedlings, inocula of five indigenous ectomycorrhizal fungi were unable to grow and associate with P. tabulaeformis seedlings. Inocula placed alongside O. davidiana seedlings, however, resulted in enhanced growth and nutritional status of O. davidiana and P. tabulaeformis seedlings, and also altered rhizosphere pH and phosphatase activity. We speculate that these species form a common mycorrhizal network and this association enhances outplanting performance of P. tabulaeformis seedlings used for forest restoration.

  2. Mycorrhizal synthesis of Tuber indicum with two indigenous hosts, Castanea mollissima and Pinus armandii.

    Science.gov (United States)

    Geng, Li-Ying; Wang, Xiang-Hua; Yu, Fu-Qiang; Deng, Xiao-Juan; Tian, Xiao-Fei; Shi, Xiao-Fei; Xie, Xue-Dan; Liu, Pei-Gui; Shen, Yu-Ying

    2009-09-01

    Tuber indicum is one of the most renowned commercialized fungi in China. Mycorrhizal investigations, however, have been carried out mainly with exotic trees. Up to now there is no detailed description of morphology of the mycorrhizae formed with the indigenous hosts of T. indicum. Containerized seedlings of two indigenous hosts of the fungus in southwestern China, Pinus armandii and Castanea mollissima, were inoculated with aqueous spore suspension of T. indicum in two kinds of substrates. Mycorrhizae began to form 4 months after inoculation and were harvested at 9 months. The contributing fungus of the mycorrhizae was confirmed to be T. indicum by morphological and ITS-rDNA sequence analyses. The morphology of emanating hyphae and epidermoid-like mantle appearance was similar to the mycorrhizae obtained with some European trees. The high morphological variation and the similarity to that of Tuber melanosporum makes it difficult to distinguish the mycorrhizae of the two species by morphology alone. The synthesis of mycorrhizae of T. indicum with its indigenous hosts will be of great significance for planned cultivation of the Asian black truffles.

  3. Microenvironmental heterogeneity of physical soil properties in a broad-leaved Pinus koraiensis forest gap

    Institute of Scientific and Technical Information of China (English)

    Wenbiao DUAN; Jing WANG; Yan LI

    2009-01-01

    Microenvironmental heterogeneity of soil phy-sical properties in 0-20 cm and 20-40 cm soil layers in a broad-leaved Pinus koraiensis forest gap in Xiao Xing'anl-ing Mountains were analyzed by geostatistical method.The results show that the amount of soil water, saturation water capacity, capillary water capacity and porosity in the top layer were greater than those in the lower layer, except for bulk density, where the opposite applied. Soil physical properties in the top soil layer had relatively higher ranges and coefficients of variation. The total and auto correlation spatial heterogeneity of soil physical properties in the top layer were larger than those in the lower layer. The soil water had a strong anisotropic structure in an easterly and northerly direction, but porosity shows isotropy in the same directions. With increasing spatial distance, the other three physical factors exhibited anisotropic structures. The mutual effect between semi-variograms of soil physical properties in the top layer within the spatial autocorrelation range was not significant. For spatial distribution of physical properties within different layers, the patches at the middle and lower ranks in the forest gap dominated.Patches at higher rank were only distributed in the 0-20 cm soil layer and were located north of the forest gap center.

  4. Loblolly pine (Pinus taeda) female gametophyte and embryo pH changes during seed development.

    Science.gov (United States)

    Pullman, Gerald S; Johnson, Shannon

    2009-06-01

    Stage-specific measurements of female gametophyte (FG) and embryo pH (hydrogen ion concentration) were made through the sequence of loblolly pine (Pinus taeda L.) seed development. The FG tissue from two open-pollinated trees showed similar pH profiles starting at 5.5 shortly after fertilization, increasing to about 6.1 at stage 7, levelling off at 6.3-6.5 towards the end of development and dropping to 6.0 just before cone opening. Measurements of the chalazal end were 0.05-0.2 pH units less than the micropylar end through early-to-mid-development. In contrast, embryo pH maintained a nearly constant value near 7.0 through development. Profiles of pH through seed development were similar whether portrayed by date or stage of embryo present in the seed. The pH profiles assisted in the development of improved embryogenic tissue initiation techniques. When post-autoclaving maturation medium pH was raised from about 5.3 in control medium to 5.7 or 5.5-5.7 with 2(n-morpholino)ethanesulphonic acid, cotyledonary embryo yields increased.

  5. Dispersal syndrome differentiation of Pinus armandii in Southwest China: Key elements of a potential selection mosaic

    Science.gov (United States)

    Chen, Fan; Chen, Jin

    2011-11-01

    Pinus armandii is a species of pine native to China with a wide geographical distribution and large-wingless seeds (about 300 mg). The study is to determine the variation in seed dispersal traits among populations within a relative small geographic scale and furthermore to explore if the trait differentiation results in the differences in dispersers, in particular nutcrackers ( Nucifraga caryocatactes) and scatter-hoarding rodents. We conducted studies at five sites at different elevations in northwest Yunnan Province. The study sites are separated by 10-200 km and divided into populations partly isolated by mountains and rivers. The cone and seed traits diverged significantly among the five study sites while the traits among individual trees at each site did not differ significantly. Nutcrackers and scatter-hoarding rodents presented conflicting preference in cone and seed traits: nutcrackers preferred smaller cones with smaller seeds, which increased the foraging efficiency of nutcrackers; while scatter-hoarding rodents tended to cache larger seeds. Consistent with variation in preferences by nutcrackers and scatter-hoarding rodents, in nutcracker-dominated sites, pines were characterized by smaller cones, smaller seeds, and thinner seed coats; while in sites where nutcrackers were not abundant, pines had relatively larger cones with larger seeds, which could enhance caching activities by scatter-hoarding rodents. The study provided some key elements for potential selection mosaic on cone and seed traits of a long-lived perennial tree among populations with limited geographical range.

  6. Propriedades de resistência e rigidez da madeira juvenil e adulta de Pinus taeda L.

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    Ballarin Adriano Wagner

    2003-01-01

    Full Text Available O objetivo do presente trabalho foi o estudo da variabilidade das propriedades de resistência e rigidez à flexão estática e à densidade aparente (12% entre a madeira juvenil e adulta de Pinus taeda L., de 37 anos de idade, procedente do Horto Florestal de Manduri, Estado de São Paulo. Na primeira parte do trabalho foram determinadas a região de madeira juvenil, a região de transição e a região de madeira adulta, por meio de estudos anatômicos (comprimento dos traqueídes axiais, segundo as recomendações das normas ABNT e IAWA. Os resultados mostraram que a região de madeira juvenil dessa espécie ocorre aproximadamente até o 18º anel de crescimento. Na segunda parte do trabalho foram analisados a resistência (módulo de ruptura - MOR à flexão, o módulo de elasticidade (MOE nessa mesma solicitação e a densidade aparente (12% para as madeiras juvenil e adulta. Os resultados mostraram que o MOE e o MOR da madeira juvenil foram menores e mais variáveis que aqueles obtidos para madeira adulta. A densidade apresentou a mesma tendência observada nas propriedades avaliadas no ensaio de flexão estática.

  7. Slow pyrolysis of wood barks from Pinus brutia Ten. and product compositions.

    Science.gov (United States)

    Sensöz, Sevgi

    2003-09-01

    Biomass in the form of pine bark (Pinus brutia Ten.) was pyrolysed in an externally heated fixed-bed reactor. The effects of temperature and heating rate on the yields and compositions of the products were investigated. Pyrolysis runs were performed using reactor temperatures between 300 and 500 degrees C with heating rates of 7 and 40 degrees Cmin(-1). The product yields were significantly influenced by the process conditions. The bio-oil obtained at 450 degrees C, at which the liquid product yield was maximum, was analysed. It was characterized by Fourier transform infrared spectroscopy. In addition, the solid and liquid products were analysed to determine their elemental composition and calorific value. Chemical fractionation of bio-oil showed that only low quantities of hydrocarbons were present, while oxygenated and polar fractions dominated. The empirical formula of the bio-oil with heating value of 31.03 MJkg(-1) was established as CH(1.43)O(0.332)N(0.0013).

  8. Introduction of Pinus radiata for afforestation: a review with reference to Aba, China

    Institute of Scientific and Technical Information of China (English)

    BIHui-quan; JackSimpson; LIRong-wei; YANHong; WUZong-xing; CAIShi-min; RobertEldridge

    2003-01-01

    Pinus radiata D. Don, native to a Californian coastal environment, has been introduced to many parts of the world as an exotic species for afforestation. It is now a major plantation species in the Southern Hemisphere. In 1990, it was introduced to the heavily degraded, dry Min river valley area in Aba prefecture of Sichuan Province, P. R. China. Survival and growth of young trees planted at several sites appear to be reasonable. This review is to serve as an introduction to the large body of literature on P.radiata for forest scientists in China. It covers the following aspects: P. radiata in its native environment and in ex situ plantations,provenance and genetic variations, environmental limitations and climate niche, diseases and pests, lessons from unsuccessful introductions, and the use of P. radiata for ecological restoration. The early growth of P. radiata planted in the dry river valley area is briefly described. Potential problems associated with the introduction of P. radiata in Aba and future research needs are also identified.

  9. Characterization of the Pinus massoniana transcriptional response to Bursaphelenchus xylophilus infection using suppression subtractive hybridization.

    Science.gov (United States)

    Xu, Liang; Liu, Zhen-Yu; Zhang, Kai; Lu, Quan; Liang, Jun; Zhang, Xing-Yao

    2013-05-28

    Pine wilt disease (PWD) caused by pine wood nematode (PWN), Bursaphelenchus xylophilus, is the most destructive diseases of pine and poses a threat of serious economic losses worldwide. Although several of the mechanisms involved in disease progression have been discovered, the molecular response of Pinus massoniana to PWN infection has not been explored. We constructed four subtractive suppression hybridization cDNA libraries by taking time-course samples from PWN-inoculated Masson pine trees. One-hundred forty-four significantly differentially expressed sequence tags (ESTs) were identified, and 124 high-quality sequences with transcriptional features were selected for gene ontology (GO) and individual gene analyses. There were marked differences in the types of transcripts, as well as in the timing and levels of transcript expression in the pine trees following PWN inoculation. Genes involved in signal transduction, transcription and translation and secondary metabolism were highly expressed after 24 h and 72 h, while stress response genes were highly expressed only after 72 h. Certain transcripts responding to PWN infection were discriminative; pathogenesis and cell wall-related genes were more abundant, while detoxification or redox process-related genes were less abundant. This study provides new insights into the molecular mechanisms that control the biochemical and physiological responses of pine trees to PWN infection, particularly during the initial stage of infection.

  10. Characterization of the Pinus massoniana Transcriptional Response to Bursaphelenchus xylophilus Infection Using Suppression Subtractive Hybridization

    Directory of Open Access Journals (Sweden)

    Xing-Yao Zhang

    2013-05-01

    Full Text Available Pine wilt disease (PWD caused by pine wood nematode (PWN, Bursaphelenchus xylophilus, is the most destructive diseases of pine and poses a threat of serious economic losses worldwide. Although several of the mechanisms involved in disease progression have been discovered, the molecular response of Pinus massoniana to PWN infection has not been explored. We constructed four subtractive suppression hybridization cDNA libraries by taking time-course samples from PWN-inoculated Masson pine trees. One-hundred forty-four significantly differentially expressed sequence tags (ESTs were identified, and 124 high-quality sequences with transcriptional features were selected for gene ontology (GO and individual gene analyses. There were marked differences in the types of transcripts, as well as in the timing and levels of transcript expression in the pine trees following PWN inoculation. Genes involved in signal transduction, transcription and translation and secondary metabolism were highly expressed after 24 h and 72 h, while stress response genes were highly expressed only after 72 h. Certain transcripts responding to PWN infection were discriminative; pathogenesis and cell wall-related genes were more abundant, while detoxification or redox process-related genes were less abundant. This study provides new insights into the molecular mechanisms that control the biochemical and physiological responses of pine trees to PWN infection, particularly during the initial stage of infection.

  11. Paleophytochemical Constituents from the Pliocene Fossil Wood of Pinus Armandii from Longling, Yunnan

    Institute of Scientific and Technical Information of China (English)

    ZHAO Youxing; LI Chengsen; YI Tiemei; LUO Xiaodong; ZHOU Jun

    2008-01-01

    Specimens of fossil wood preserved lignified in Pliocene brown coal and identified as Pinus armandii Francher come from an opencast coalmine at Longling in western Yunnan Province, China. Phytochemical investigation of the fossil wood isolated using liquid column chromatography seven compounds (1-7) including a new fluorene derivative named 11,11-dimethy1-11H-benzo[b]fluorene.A further 28 volatiles were detected by gas chromatogrphy-mass spectrometry(GC-MS).Spectroscopic investigation methods,including MS and 1D and 2D-NMR techniques eucidated the structure of the seven compounds.Two types of natural products,isopimara and stilbene commonly occuring in extant and pliocene fossil p. armandii indicate phytochemical fidelith during burial under certain circumstances in sediments.Discovery of stilbenes that can inhibit the activities of wood-destroying fungi in the pliocene p.armandii prompts the assumption that the chemical preservation of this pliocene fossil wood of p.armandii in brown coal might contribute to the presence of inne natural inhibitors against wood-destroying fungi.

  12. Physiological responses of needles of Pinus massoniana elite families to phosphorus stress in acid soil

    Institute of Scientific and Technical Information of China (English)

    HE You-lan; LIU Ai-qin; Mulualem Tigabu; WUPeng-fei; MA Xiang-qing; WANG Chen; Per Christer Oden

    2013-01-01

    Pinus massoniana Lamb.is a major timber species widely planted in the South China,where the soil is acidic and deficient in phosphorus (P) due to fixation by aluminum and iron.Understanding the physiological responses to rhizospheric insoluble P is essential for enhancing plantation productivity.Thus,a sand culture experiment was conducted with four levels of P treatment (0,5,20 g insoluble P and 10 g soluble P),and 11 P.massoniana elite families.Physiological responses were measured after two months of stress.Compared to the normal soluble P treatment,the insoluble P treatment significantly reduced the proline content and the APase activity in the needles,while it significantly increased the catalase activity by 1.3-fold and malondialdehyde content by 1.2-fold.Soluble protein content was unaffected by the treatments,but chlorophyll content was significantly lower in P-deprived treatment compared with soluble and insoluble P treatments.These physiological responses also exhibited highly significant variation among families (p < 0.01).The findings suggest that increased catalase activities in the presence of insoluble P might be involved in the activation of an anti-oxidation defense mechanism that scavenges the reactive oxygen species elicited by the stress.And this response has a strong genetic control that can be exploited to identify desirable genotypes.

  13. SOME NEEDLE CONTENTS AND VOLATILE ORGANIC COMPOUNDS EMITTED BY PINUS BRUTIA IN RELATION TO HERBIVORE ATTACK

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    G. SEMİZ

    2014-06-01

    Full Text Available Herbivores can cause many types of damage to plants. Caterpillars ingest small sections of the leaves, while others feed on specific parts of the leaf material. In this point, essential oils from coniferous trees contain secondary metabolites that act as feeding deterrent for a great number of herbivore insect species. Attacks by herbivores elicit changes in the bouquet of volatiles released by plants. Terpenoid chemicals exist both as constitutive and massively induced defenses in conifers. Hereby we studied the factors contributing to the specificity of induced defensive responses in economically important pine species of Turkey, Pinus brutia Ten., against most famous pest, pine processionary moth (Thaumetopoea wilkinsoni Tams. We quantified volatile organic compounds (VOCs emissions of needle and some other needle contents. Needle feeding by the caterpillar increased emissions of VOCs. We discuss the possible mechanisms responsible for reducing the tree's signalling capacity triggered by Th. wilkinsoni oviposition and how enhancement/suppression of VOCs can influence the interaction between the tree, the pest and other biotic/abiotic factors in environment.

  14. Golgi enrichment and proteomic analysis of developing Pinus radiata xylem by free-flow electrophoresis.

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    Harriet T Parsons

    Full Text Available Our understanding of the contribution of Golgi proteins to cell wall and wood formation in any woody plant species is limited. Currently, little Golgi proteomics data exists for wood-forming tissues. In this study, we attempted to address this issue by generating and analyzing Golgi-enriched membrane preparations from developing xylem of compression wood from the conifer Pinus radiata. Developing xylem samples from 3-year-old pine trees were harvested for this purpose at a time of active growth and subjected to a combination of density centrifugation followed by free flow electrophoresis, a surface charge separation technique used in the enrichment of Golgi membranes. This combination of techniques was successful in achieving an approximately 200-fold increase in the activity of the Golgi marker galactan synthase and represents a significant improvement for proteomic analyses of the Golgi from conifers. A total of thirty known Golgi proteins were identified by mass spectrometry including glycosyltransferases from gene families involved in glucomannan and glucuronoxylan biosynthesis. The free flow electrophoresis fractions of enriched Golgi were highly abundant in structural proteins (actin and tubulin indicating a role for the cytoskeleton during compression wood formation. The mass spectrometry proteomics data associated with this study have been deposited to the ProteomeXchange with identifier PXD000557.

  15. Pinus mugo Krummholz Dynamics During Concomitant Change in Pastoralism and Climate in the Central Apennines

    Directory of Open Access Journals (Sweden)

    Li Dai

    2017-02-01

    Full Text Available The dynamics of Pinus mugo krummholz during concomitant change in pastoral land use and climate in central Italy since the mid-20th century was investigated. Krummholz dynamics were detected using sequential aerial photography and fitted to a logistic regression model with elevation, grazing, proximity to beech forest, and proximity to krummholz as explanatory variables. Dendrochronological series were correlated with temperature and precipitation and fitted to a linear model. During this period krummholz doubled in extent and migrated 35–65 m upslope. Expansion was positively associated with krummholz proximity, residual pastoral grazing, and proximity to beech forest beyond 10 m and negatively associated with elevation and beech forest closer than 10 m. The logistic regression model forecasts krummholz migration by an additional 30 m upslope by 2060. During the 20th century, winter and spring minimum temperatures increased but did not result in increased radial stem growth of P. mugo. The combined evidence suggests that krummholz dynamics can be explained by the legacy of summer pastoralism and the dispersal limitations of P. mugo, rather than by climate change.

  16. The hydraulic conductivity of the xylem in conifer needles (Picea abies and Pinus mugo).

    Science.gov (United States)

    Charra-Vaskou, Katline; Mayr, Stefan

    2011-08-01

    Main resistances of the plant water transport system are situated in leaves. In contrast to angiosperm leaves, knowledge of conifer needle hydraulics and of the partitioning of resistances within needles is poor. A new technique was developed which enabled flow-meter measurements through needles embedded in paraffin and thus quantification of the specific hydraulic conductivity (K(s)) of the needle xylem. In Picea abies, xylem K(s) of needle and axes as well as in needles of different age were compared. In Pinus mugo, resistance partitioning within needles was estimated by measurements of xylem K(s) and leaf conductance (K(leaf), measured via 'rehydration kinetics'). Mean K(s) in P. abies needles was 3.5×10(-4) m(2) s(-1) MPa(-1) with a decrease in older needles, and over all similar to K(s) of corresponding axes xylem. In needles of P. mugo, K(s) was 0.9×10(-4) m(2) s(-1) MPa(-1), and 24% of total needle resistance was situated in the xylem. The results indicate species-specific differences in the hydraulic efficiency of conifer needle xylem. The vascular section of the water transport system is a minor but relevant resistance in needles.

  17. Essential-Oil Variability in Natural Populations of Pinus mugo Turra from the Julian Alps.

    Science.gov (United States)

    Bojović, Srdjan; Jurc, Maja; Ristić, Mihailo; Popović, Zorica; Matić, Rada; Vidaković, Vera; Stefanović, Milena; Jurc, Dušan

    2016-02-01

    The composition and variability of the terpenes and their derivatives isolated from the needles of a representative pool of 114 adult trees originating from four natural populations of dwarf mountain pine (Pinus mugo Turra) from the Julian Alps were investigated by GC-FID and GC/MS analyses. In total, 54 of the 57 detected essential-oil components were identified. Among the different compound classes present in the essential oils, the chief constituents belonged to the monoterpenes, comprising an average content of 79.67% of the total oil composition (74.80% of monoterpene hydrocarbons and 4.87% of oxygenated monoterpenes). Sesquiterpenes were present in smaller amounts (average content of 19.02%), out of which 16.39% were sesquiterpene hydrocarbons and 2.62% oxygenated sesquiterpenes. The most abundant components in the needle essential oils were the monoterpenes δ-car-3-ene, β-phellandrene, α-pinene, β-myrcene, and β-pinene and the sesquiterpene β-caryophyllene. From the total data set of 57 detected compounds, 40 were selected for principal-component analysis (PCA), discriminant analysis (DA), and cluster analysis (CA). The overlap tendency of the four populations suggested by PCA, was as well observed by DA. CA also demonstrated similarity among the populations, which was the highest between Populations I and II.

  18. Characterization of glutathione S-transferase from dwarf pine needles (Pinus mugo Turra).

    Science.gov (United States)

    Schröder, P; Rennenberg, H

    1992-09-01

    Glutathione S-transferase activity conjugating xenobiotics with glutathione (GSH) was found in extracts from needles of dwarf pine (Pinus mugo Turra). In vivo incubation of needle segments with the herbicide fluorodifen at 25 degrees C resulted in conversion of the xenobiotic to water-soluble products at initial rates of 0.7 nmol h(-1) g(fw) (-1). At 15 degrees C, the initial rate of product formation was decreased to 0.1 nmol h(-1) g(fw) (-1). In vitro conjugation studies with chloro-2,4-dinitrobenzene (CDNB) and 1,2-dichloro-4-nitrobenzene (DCNB) as model substrates gave apparent K(m) values of 0.5 mM GSH and 1.14 mM CDNB in the GSH/CDNB system and 0.3 mM GSH and 0.44 mM DCNB in the GSH/DCNB system. The pH optimum was between 7.7 and 7.9 for both the GSH/CDNB and the GSH/DCNB systems. The temperature optimum for these model substrates was between 30 and 35 degrees C, and only minute amounts of enzyme activity were detected at 15 degrees C. The activation energy in the temperature range of 15 to 30 degrees C was 46 kJ mol(-1). Dwarf pine glutathione S-transferase exhibited an approximate molecular weight of 52 kD.

  19. Evaluation of acute and sub-acute toxicity of Pinus eldarica bark extract in Wistar rats

    Directory of Open Access Journals (Sweden)

    Akram Ghadirkhomi

    2016-08-01

    Full Text Available Objective: Pinus eldarica (P. eldarica is one of the most common pines in Iran which has various bioactive constituents and different uses in traditional medicine. Since there is no documented evidence for P. eldarica safety, the acute and sub-acute oral toxicities of hydroalcoholic extract of P. eldarica bark were investigated in male and female Wistar rats in this study. Materials and Methods: In the acute study, a single dose of extract (2000 mg/kg was orally administered and animals were monitored for 7 days. In the sub-acute study, repeated doses (125, 250 and 500 mg/kg/day of the extract were administered for 28 days and biochemical, hematological and histopathological parameters were evaluated. Results: Our results showed no sign of toxicity and no mortality after single or repeated administration of P. eldarica. The median lethal dose (LD50 of P. eldarica was determined to be higher than 2000 mg/kg. The mean body weight and most of the biochemical and hematological parameters showed normal levels.  There were only significant decreases in serum triglyceride levels at the doses of 250 and 500 mg/kg of the extract in male rats (pConclusion: Oral administration of the hydroalcoholic extract of P. eldarica bark may be considered as relatively non-toxic particularly at the doses of 125 and 250 mg/kg.

  20. Hygroscopicity of wood from Eucalyptus grandis and Pinus taeda subjected to thermal treatment

    Directory of Open Access Journals (Sweden)

    Karina Soares Modes

    2013-03-01

    Full Text Available This study aimed to investigate the effect of the thermal treatment technique under two conditions—autoclave combined with electric oven and electric oven only—on the hygroscopic properties of wood from Pinus taeda L. and Eucalyptus grandis W. Hill ex Maiden, comparing them with results obtained for untreated wood. Three trees at age 25 years were sampled, with boards being removed at DBH level in order to make specimens 2.5 x 2.5 x 5.0 cm in size in the radial, tangential and axial direction respectively. In the combined treatment, specimens were subjected to thermal treatment in an autoclave set at 130°C/± 3°C and kgf/cm² pressure for 3 hours, then to a conditioning period, followed by heat in an electric oven set at 160°C/±1°C for the same time. Properties being evaluated included water absorption rate, volumetric swelling, water repellency effectiveness and anti-swelling efficiency. Results revealed that the thermal treatments being adopted were effective in reducing hygroscopicity and in increasing the dimensional stability of both species, revealing also that the combined autoclave and electric oven treatment provides best results.

  1. [Relationships between understory vegetation coverage and environmental factors in Pinus massoniana plantations from aerial seeding].

    Science.gov (United States)

    Zhao, Fang; Ouyang, Xun-zhi

    2015-04-01

    The relationships between understory vegetation coverage and environmental factors in Pinus massoniana plantations from aerial seeding were studied by using principal component analysis, redundancy analysis and variation partitioning. The selected environmental factors in total explained 74.2% variation of the understory vegetation coverage. At low altitude, stand characteristics were the key factor to influence the understory vegetation coverage. Stand characteristics, soil property and topographic factor were respectively explained 55.0% (including 29.1% for separateness and 25.9% for interaction with other factors), 38. 9% (including 12.1% for separateness and 26.8% for interaction with other factors) and 9.0% (including 5.6% for separateness and 3.4% for interaction with other factors) of the total variation. Average diameter at breast height and canopy density affected mostly and positively correlated with the coverage of bryophyta, graminoid and shurb groups at significant level. Noncapillary porosity and soil water content showed a highly significant positive correlation to dicranopteris coverage, whereas average diameter at breast height, canopy density, soil nutrients and enzyme activity significantly negatively correlated with it. The coverage of graminoid, bryophyta and shurb groups showed the positive correlations, which indicated the three groups could promote each other, while the dicranopteris coverage had significant negative correlation with the three groups mentioned above.

  2. Larvicidal and mosquito repellent activities of Pine (Pinus longifolia, Family: Pinaceae oil

    Directory of Open Access Journals (Sweden)

    M.A. Ansari, P.K. Mittal, R.K. Razdan & U. Sreehari

    2005-09-01

    Full Text Available Background & objectives: Various plant-based products are safe and biodegradable alternatives tosynthetic chemicals for use against mosquitoes. Oil of Pinus longifolia is traditionally used forprotection against mosquitoes in some rural areas but there is no documented report of its use againstmosquitoes. The present study was undertaken to scientifically evaluate the activity of Pine oilagainst mosquitoes.Methods: The oil was procured from the market and its contents were chemically analysed. Larvicidalactivity of oil was tested in laboratory bioassays, while repellent action was studied during wholenight bait collections in field by direct application on the skin and after its impregnation on mats.Results: Results showed varying degree of larvicidal activity of Pine oil against mosquitoes with LC50values ranging between 82 and 112 ppm. The Pine oil had strong repellent action against mosquitoesas it provided 100% protection against Anopheles culicifacies for 11 h and 97% protection againstCulex quinquefasciatus for nine hours respectively. Electrically heated mats prepared from Pine oilprovided, 94 and 88% protection against An. culicifacies and Cx. quinquefasciatus for 10 and sevenhours respectively.Interpretaion & conclusion: Pine oil is effective against mosquito larvae at very higher doses whichare not of any practical utility. However, Pine oil showed strong repellent action against An. culicifacies(malaria vector and Cx. quinquefasciatus (pest mosquito. Thus its use could be popularised asmosquito repellent.

  3. Effects of long-term elevated atmospheric CO{sub 2} concentrations on Pinus ponderosa

    Energy Technology Data Exchange (ETDEWEB)

    Surano, K.A.; Kercher, J.R. [eds.

    1993-10-01

    This report details the results from an experiment of the effects of long-term elevated atmospheric CO{sub 2} concentrations on ponderosa pine (Pinus ponderosa Laws.) saplings and seedlings. The study began in 1983 as a pilot study designed to explore the feasibility of using open-top chambers for continuous multi-year exposures on sapling-sized trees and to examine possible CO{sub 2} responses so that future research could be adequately designed. however, following the first year of exposure, preliminary results from the study indicated that measurements of CO{sub 2} responses should be intensified. Open-top chambers proved suitable for use in multiyear exposures of mature trees. With respect to the preliminary examination of CO{sub 2} responses, many interesting observations were made. The nature of the preliminary results suggests that future long-term field CO{sub 2} exposures on perennial species may be critical to the understanding and preparation for future environments. Other research reported here attempted to adapt an existing western coniferous forest growth and succession model for use in elevated CO{sub 2} scenarios using differential species responses, and assessed the usefulness of the model in that regard. Seven papers have been processed separately for inclusion in the appropriate data bases.

  4. PROPERTIES OF THERMO-MECHANICALLY TREATED WOOD FROM PINUS CARIBAEA VAR. HONDURENSIS

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    Cristiane Moreira Tavares Santos,

    2012-02-01

    Full Text Available This study aimed at evaluating the effect of thermo-mechanical treatment on properties of Pinus caribaea var. hondurensis wood. Two pressure levels (25% and 50% of the compression strength perpendicular to grain were evaluated. The treatment was applied in a laboratory hot press in one-step or two-step modes for 50 minutes. In the one-step treatment, the total pressure was applied after the temperature of the center of the wood reached 170°C. In the two-steps treatment, half of the pressure was applied after the center of the wood reached 100°C, and the final pressure was applied when it reached 170°C. The weight loss immediately after treatment was equivalent to the wood moisture content, indicating that degradation of wood polymers did not occur. However, the treatments showed decreasing values of the moisture content, which were reduced from 12.3% to 9.8%. A moderate improvement on surface roughness was achieved, while wood wettability was highly reduced in all treatments, as determined by contact angle measurement. On the other hand, the treatment applied did not improve the wood dimensional stability, but all mechanical properties presented a trend of improvement.

  5. EFFECTS OF HEAT TREATMENT ON CALABRIAN PINE (PINUS BRUTIA TEN. WOOD

    Directory of Open Access Journals (Sweden)

    Saim Ates

    2009-08-01

    Full Text Available In this study, the effects of heat treatment on some physical, mechanical, chemical properties, and cellulose crystallinity of calabrian pine (Pinus brutia Ten. were evaluated. Wood specimens were treated with heat under atmospheric pressure at three different temperatures (130, 180, and 230 oC and two different time levels (2 and 8 h. Air-dry density (Dm, oven-dry density (D0, shrinkage (β, swelling (α, fiber saturation point (FSP, compression strength parallel to grain (σc//, bending strength (σb, modulus of elasticity (MOE in bending, equilibrium moisture content (EMC, holocellulose, and alcohol solubility were decreased, whereas 1% NaOH solubility and lignin content were increased, depending on the heating temperature and time. Cellulose crystallinity of the samples was not changed significantly. 130 oC showed a minimal effect; on the other hand, 230 oC showed a maximum effect on all properties of the treated wood. That’s why, for the heat treatment process, 130 oC for 2 h should be applied in situations where mechanical properties such as modulus of elasticity, compression strength, bending strength, and hardness are important. However, 230 oC for 2 h should be used in situations where it is preferred to obtain favorable physical properties, such as density, shrinkage, swelling, and moisture content.

  6. PASTAS ESTIMULANTES EM SISTEMAS DE RESINAGEM DE Pinus elliottii var. elliottii

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    André Luiz Marretto Fusatto

    2013-01-01

    Full Text Available The use of stimulating pastes aims to increase the rate of resin flow and its time of duration. In the Forest Experiment Station of Itatinga, São Paulo state, 159 trees of Pinus elliottii var. elliottii were were submitted to the treatments with 5 stimulant pastes, (Red Alchem, Paste with Ethephon Planebrás, Black Resin Brazil, Red Eldorado and Test Paste (Paraquat + Ethephon that was compared to the Control (Black Planebrás. Biweekly chipping and collects every three months were accomplished (from the result of 5 grooves, being determined the resin weight during 2.5 years. The application of stimulating pastes with ethephon did not show significant advantage about application of pastes without ethephon. The stimulant pastes applied were different in the weightings performed. In the treatments applied, the percentage of pitch ranged from 71.33% to 76.56% and the turpentine ranged from 10.37% to 13.78%. The yield of alpha-pinene ranged from 37.70% to 53.31%, and of beta-pinene from 34.34% to 50.41%.

  7. Molecular identification of white morphotype strains of endophytic fungi from Pinus tabulaeformis.

    Science.gov (United States)

    Guo, Liang Dong; Huang, Guo R; Wang, Yu; He, Wen H; Zheng, Wei H; Hyde, Kevin D

    2003-06-01

    Sterile mycelia isolated from Pinus tabulaeformis were grouped into white morphotype strains based on cultural characteristics. Eighteen of the isolates were randomly selected and identified to various taxonomic levels based on nuclear ribosomal DNA (nrDNA) sequence analysis. The 5.8S gene and flanking internal transcribed spacer (ITS1 and ITS2) regions of nrDNA were amplified and sequenced. Phylogenetic analysis of the 5.8S gene sequences indicated that the white morphotype strains were Ascomycota. Further identification was achieved by means of sequence similarity comparison and phylogenetic analysis of the ITS regions. Results showed that strains WMS9 and WMS10 were Lophodermium species (Rhytismataceae), while strains WMS11, WMS13 and WMS18 were species of Rhytismataceae. Strains WMS2, WMS3, WMS4, WMS5 and WMS6 were identified to Rosellinia, strain WMS1 to Entoleuca, and strain WMS14 to Nemania (Xylariaceae). Strains WMS7, WMS8, WMS12, WMS15, WMS16 and WMS17 were xylariaceous species. The potential of using DNA sequence analysis in the identification of endophytic fungi is discussed.

  8. An assessment of nitrogen saturation in Pinus banksiana plots in the Athabasca Oil Sands Region, Alberta

    Directory of Open Access Journals (Sweden)

    Shaun A. WATMOUGH

    2010-08-01

    Full Text Available During the past 15 years, there has been a dramatic increase in the amount of reactive nitrogen (N in the atmosphere, leading to concerns that chronic elevated N deposition may result in negative effects on natural ecosystems. This study examines the response of jack pine (Pinus banksiana plots to N air concentrations within the Athabasca Oil Sands Region (AOSR in northern Alberta, which has experienced elevated N emissions since the 1990s. Air concentrations of nitrogen dioxide (NO2, ammonia, and nitric acid at the study plots are generally low although NO2 is strongly correlated with sulphur dioxide indicating an exposure gradient associated with industrial emissions. Nitrogen concentrations in P. banksiana foliage and two lichen indicator species (Hypogymnia physodes and Evernia mesomorpha were significantly correlated with annual NO2 exposure. Relationships between NO2 (or N exposure and other aspects of N cycling were less evident. Nitrogen content and carbon to nitrogen ratio in the forest floor and soil or potential net N mineralization rates were not correlated with N exposure. Nitrification was negligible suggesting efficient ecosystem immobilization of current N deposition. Based on the response of foliage to N exposure, sites closest to industrial activity appear to be in the early stages of N saturation.

  9. Content Changes of Several Endogenous Plant Hormones in Female-Sterile Pinus tabulaeformis Carr.

    Institute of Scientific and Technical Information of China (English)

    2005-01-01

    To determine the relationship between female sterility of Pinus tabulaeformis Carr. and plant hormones, content changes of several endogenous plant hormones in a female-sterile clone and a normal clone were analyzed during the key period of female gametophyte abortion in mutant clones. The change of ABA content showed a similar pattern between normal and mutant clones, but the ABA content of the mutant clone was always much higher than that of the normal clone. In the normal clone, the IAA content decreased significantly at the early stage of the period and reached its minimum during the first ten days of April and stabilized thereafter. The endogenous ZR levels increased sharply at the early stages and reached a maximum in the middle of April, then decreased dramatically. The IAA and ZR contents of the mutant clone did not change perceptively through the entire period. We conclude that the high levels of ABA and the deficit of IAA and ZR may be the reasons of female gametophyte abortion in the female-sterile clones of P. tabulaeformis.

  10. Monoterpene synthases of loblolly pine (Pinus taeda) produce pinene isomers and enantiomers.

    Science.gov (United States)

    Phillips, M A; Savage, T J; Croteau, R

    1999-12-01

    The turpentine fraction of conifer oleoresin is a complex mixture of monoterpene olefins and plays important roles in defense and in the mediation of chemical communication between conifer hosts and insect predators. The stereochemistry of the turpentine monoterpenes is critical in these interactions, influencing host recognition, toxicity, and potency of derived pheromones, and the stereochemical composition of these compounds lends insight into their biogenetic origin, with implications for the numbers and types of enzymes responsible and their corresponding genes. Analysis of the oleoresin from several tissues of loblolly pine (Pinus taeda) showed the derived turpentine to consist mainly of (+)-(3R:5R)-alpha-pinene and (-)-(3S:5S)-beta-pinene. Cell-free extracts from xylem tissue yielded three monoterpene synthases which together account for the monoterpene isomer and enantiomer content of the turpentine of this tissue. The major products of these enzymes, produced from the universal precursor of monoterpenes, geranyl diphosphate, were shown to be (+)-alpha-pinene, (-)-alpha-pinene, and (-)-beta-pinene, respectively. In most properties (molecular mass of approximately 60 kDa, K(m) for geranyl diphosphate of 3 microM, requirement for monovalent and divalent cations), these enzymes resemble other monoterpene synthases from conifer species.

  11. Influences of antibiotics on plantlet regeneration via organogenesis in loblolly pine (Pinus taeda L.)

    Institute of Scientific and Technical Information of China (English)

    TANG Wei; Latoya Harris; Ronald J. Newton

    2003-01-01

    Three antibiotics ampicillin, carbenicillin, and cefotaxime were evaluated for their effects on induction, growth, and differentiation of organogenic calli, as well as rooting of regenerated shoots of three loblolly pine (Pinus taeda L.) genotypes. Of the antibiotics administered, cefotaxime maximally increased the frequency of callus formation and growth rate of organogenic calli, carbenicillin maximally increased the frequency of shoot regeneration and the average number of adventitious shoots per piece of organogenic callus, ampicillin maximally decreased the rooting frequency of regenerated shoots and mean number of roots per regenerated shoot, in comparison with antibiotic-free media. Compared with the control, ampicillin minimally increased the frequency of callus formation, cefotaxime minimally increased the frequency of shoot regeneration, and carbenicillin minimally decreased the rooting frequency of regenerated shoots in three loblolly pine genotypes tested. All three antibiotics increased the frequencies of callus formation and shoot regeneration, and reduced the rooting frequency of regenerated shoots suggested that the establishment of an efficient Agrobacterium tumefaciens-mediated transformation protocol for stable integration of foreign genes into loblolly pine need to select a suitable antibiotic. This investigation could be useful for optimizing genetic transformation of conifers.

  12. Development and growth of plantlets of Pinus contorta regenerated from adventitious buds

    Energy Technology Data Exchange (ETDEWEB)

    Flygh, G.; Groenroos, R.; Arnold, S. von [Swedish Univ. of Agricultural Sciences, Uppsala (Sweden). Dept. of Forest Genetics; Hoegberg, K.A. [The Association for Forest Tree Breeding, Svaloev (Sweden)

    1998-11-01

    Before micropropagation techniques can be applied to a particular species, it is crucial to optimize the method and to determine how the micropropagated plants grow in the field. Adventitious shoots developed on embryos of Pinus contorta Dougl. ex Loud. after a 2 h pulse treatment with 250 {mu}M N6-benzyladenine. The time to first subculture after the pulse treatment influenced the yield of adventitious shoots. On average, 68% of the adventitious shoots had developed roots 12 weeks after treatment with 1.25 mM indole-3-butyric acid for 6 h. The auxin treatment stimulated early rooting (i.e. within 6 weeks) but had no effect on late rooting (i.e. after 6 weeks). The size of the plantlets was of importance for the survival when potted. All plantlets with a distinct stem elongated during the first growth period while some without did not. The relative height growth rate of plantlets was similar to that of seedlings. In the field the increase of height was similar for plantlets and seedlings. Plagiotropy was higher for the plantlets than for the seedlings (35 and 10% respectively). We concluded that most plantlets of P. contorta elongate normally and have a similar gross morphology to seedlings 23 refs, 8 figs, 4 tabs

  13. Effects of ozone and climate on ponderosa pine (Pinus ponderosa) growth in the Colorado Rocky Mountains

    Energy Technology Data Exchange (ETDEWEB)

    Peterson, D.L. (Washington Univ., Seattle, WA (United States)); Arbaugh, M.J.; Robinson, L.J. (United States Dept. of Agriculture Forest Service, Riverside, CA (United States))

    1993-01-01

    Long-term radial growth trends of ponderosa pine (Pinus ponderosa var. scopulorum) were studied in second-growth stands in the Front Range of the Colorado Rocky Mountains to determine if there has been any impact from oxidant air pollution. Although ozone concentrations are relatively high at some locations, visible pollutant injury was not found in any trees. Time series of basal area increments are generally homogeneous within stands. Concurrent periods of increasing and decreasing growth can be found in stands throughout the Front Range, which indicates that there are temporal growth trends at the regional level. Most of these trends appear to be related to the effects of stand dynamics and climate. Correlation analysis with climatic variables indicates that soil moisture supply is the dominant factor controlling interannual variation of basal area growth. Palmer hydrological drought index is highly correlated (positively) with growth during the summer months; total precipitation in spring is positively correlated with growth, and mean temperature in spring is negatively correlated with growth. There are no recent changes in growth trends that might be associated with elevated levels of ambient ozone in the Front Range. 66 refs., 5 figs., 1 tab.

  14. Evidence for selection in response to radiation exposure: Pinus sylvestris in the Chernobyl exclusion zone

    Energy Technology Data Exchange (ETDEWEB)

    Kuchma, Oleksandra, E-mail: oleksandra.kuchma@googlemail.com [Forest Genetics and Forest Tree Breeding, Buesgen Institute, Georg-August University Goettingen, Buesgenweg 2, Goettingen 37077 (Germany); Finkeldey, Reiner, E-mail: rfinkel@gwdg.de [Forest Genetics and Forest Tree Breeding, Buesgen Institute, Georg-August University Goettingen, Buesgenweg 2, Goettingen 37077 (Germany)

    2011-06-15

    Changes of genetic structures due to viability selection are likely to occur in populations exposed to rapidly and extremely changing environmental conditions after catastrophic events. However, very little is known about the extent of selective responses and in particular the proportion of the genome involved in putatively adaptive reactions for non-model plants. We used amplified fragment length polymorphisms (AFLPs) in order to investigate genetic differences between pine (Pinus sylvestris) trees which were partially exposed to extreme environmental conditions. Genetic variation patterns of pines exposed to high radiation in the Chernobyl exclusion zone with or without phenotypic stress symptoms were compared to control trees with a similar origin. Six percent of the investigated loci (15 of 222 loci) were identified as candidates for selective responses. Moderate differentiation was observed between groups of trees showing either weak or strong phenotypic responses to high radiation levels. - Highlights: > Genetic variation patterns of pines exposed to high radiation were investigated. > Pines with or without phenotypic stress symptoms were compared to control trees. > AFLP markers were used to reveal evidences of selection processes. > 15 of 222 loci are identified as candidates for selective responses. > Moderate differentiation is observed between irradiated and control trees. - Genetic responses to the exposure of trees to radiation in the Chernobyl zone may involve adaptive changes at a comparatively large part of the genome.

  15. Influences of excessive Cu on photosynthesis and growth in ectomycorrhizal Pinus sylvestris seedlings

    Institute of Scientific and Technical Information of China (English)

    HUANG Yi; TAO Shu

    2004-01-01

    Growth and photosynthesis responses were measured for Scots pine(Pinus sylvestris L. cv.) inoculated with ectomycorrhizal fungi(Suillus bovinus) under 6.5 and 25 mg/L Cu treatments to evaluate ectomycorrhizal seedlings' tolerance to heavy metal stress. Results showed that excessive Cu can significantly impair the growth and photosynthesis of pine seedlings, but such impairment is much smaller to the ectomycorrhizal seedlings. Under 25 mg/L Cu treatment, the dry weight of ectomycorrhizal seedlings is 25% lower than the control in contrary to 53% of the non-mycorrhizal seedlings, and the fresh weight of ectomycorrhizal roots was significantly higher than those of non-mycorrhizal roots, about 25% and 42% higher at 6.5 and 25 mg/L Cu treatments respectively. Furthermore, ectomycorrhizal fungi induced remarkable difference in the growth rate and pigment content of seedlings under excessive Cu stress. At 25 mg/L Cu, the contents of total chlorophyll, chlorophyll-a and chlorophyll-b were 30% higher in ectomycorrhizal plants than those in non-mycorrhizal plants. O2 evolution and electron transport of PSI and PSII were restrained by elevated Cu stress. However, no significant improvement was observed in reducing the physiological restraining in ectomycorrhizal seedlings over the non-mycorrhizal ones.

  16. Production, composition and toxicology studies of Enzogenol® Pinus radiata bark extract.

    Science.gov (United States)

    Frevel, Mathias A E; Pipingas, Andrew; Grigsby, Warren J; Frampton, Chris M; Gilchrist, Nigel L

    2012-12-01

    Enzogenol® pine bark extract is a dietary supplement and food ingredient produced by water extraction of Pinus radiata. We present production method, composition, and safety data from rat and dog toxicological and human clinical studies. The dry powder contains proanthocyanidins (>80%), taxifolin (1-2%), other flavonoids and phenolic acids (up to 8%), and carbohydrates (5-10%). Reverse mutation assays showed lack of mutagenic activity. Single and 14-day repeat dosing in rats and dogs had no influence on body weight, feed consumption, blood chemistry, and haematology at any dose level. There were no treatment related findings on gross and detailed necroscopy, organ weights, organ weight ratios and histology. The only adverse events were emesis and diarrhoea in dogs occurring mainly in un-fed condition and at the highest dose level in a total of 18% of applications. The MTD and NOAEL in the present rat and dog studies were 2500 and 750 mg/kg/day, respectively. Consumption of 480 mg/day for 6 months and 960 mg/day for 5 weeks in two human studies showed Enzogenol® had no adverse influence on liver and kidney function, haematology, and did not cause any adverse events. Our studies indicate lack of toxicity of Enzogenol® and support safe use as a food ingredient.

  17. Biological activities of procyanidins from the bark of Pinus caribaea Morelet.

    Science.gov (United States)

    Zhang, Zhengge; Du, Xueyu; Wang, Fei

    2009-01-01

    The extraction and biological activities of procyanidins from the bark of Pinus caribaea Morelet (P. caribaea) have been studied for the first time in our research. The main factors influencing the extraction, including solvent, temperature and liquid-to-solid (L/S) ratio, were discussed. The results illustrate that the suitable conditions for the extraction of procyanidins from the bark of P. caribaea are as follows: extraction temperature at 50 degrees C, L/S ratio at 11 : 1 and ethanol volume fraction at 70%. The focus of this study is to investigate the biological activities of the procyanidins from the bark of P. caribaea. Free-radical scavenging activity of the procyanidins was measured by means of 2,2-diphenyl-1-picrylhydrazyl (DPPH) method, and it was clear that the procyanidins product had a strong radical scavenging ability. Furthermore, the inhibition effect of the procyanidins from the bark of P. caribaea on different human cancer cells cultured in vitro was investigated. The results indicated that the procyanidins extracted with water had a stronger inhibition on promycelocytic cells HL-60, an effective inhibition on human stomach adenocarcinoma cells BGC-823 and human hepatocellular carcinoma cells BEL-7402, but no effect on human lung carcinoma cells A549, whereas the procyanidins extracted with ethanol had an effective inhibition on HL-60 and BGC-823, but no effect on A549 or BEL-7402.

  18. In vitro mycorrhization and acclimatization of Amanita caesareoides and its relatives on Pinus densiflora.

    Science.gov (United States)

    Endo, Naoki; Gisusi, Seiki; Fukuda, Masaki; Yamada, Akiyoshi

    2013-05-01

    Amanita caesareoides is a sister species of Amanita caesarea, also known as Caesar's mushroom and one of the most desirable edible mycorrhizal mushrooms. However, cultivation of Caesar's mushrooms has not yet been successful due to the difficulties involved in establishing pure cultures. In this study, we established pure cultures of four Asian Caesar's mushroom species, i.e., A. caesareoides, Amanita javanica, Amanita esculenta, and Amanita similis, which were identified by sequence analysis of their rDNA internal transcribed spacer (ITS) region. Five selected isolates in A. caesareoides, A. javanica, and A. esculenta were tested for ectomycorrhizal syntheses with axenic Pinus densiflora seedlings in vitro. Ectomycorrhizal tips of each fungal isolate tested were observed on pine lateral roots within 5 months of inoculation. Seventeen pine seedlings that formed ectomycorrhizas in vitro with these three Amanita species were acclimatized under non-sterile conditions. Seven months following acclimatization, ectomycorrhizal colonization by A. caesareoides was observed on newly grown root tips, which was confirmed by polymerase chain reaction restriction fragment length polymorphism analysis of the fungal rDNA ITS region. Two other Amanita species also survived during ectomycorrhizal acclimatization. These results suggest that the cultivation of A. caesareoides and its relatives can be attempted through mycorrhizal synthesis using P. densiflora as a host. This is the first report of in vitro mycorrhization of Asian Caesar's mushrooms and their acclimatization under non-sterile conditions.

  19. Pollen tube reuses intracellular components of nucellar cells undergoing programmed cell death in Pinus densiflora.

    Science.gov (United States)

    Hiratsuka, Rie; Terasaka, Osamu

    2011-04-01

    Through the process known as programmed cell death (PCD), nucelli of Pinus densiflora serve as the transmitting tissue for growth of the pollen tube. We sought to clarify the processes of degradation of nucellar cell components and their transport to the pollen tube during PCD in response to pollen tube penetration of such nucelli. Stimulated by pollination, synthesis of large amounts of starch grains occurred in cells in a wide region of the nucellus, but as the pollen tube penetrated the nucellus, starch grains were degraded in amyloplasts of nucellar cells. In cells undergoing PCD, electron-dense vacuoles with high membrane contrast appeared, assumed a variety of autophagic structures, expanded, and ultimately collapsed and disappeared. Vesicles and electron-dense amorphous materials were released inside the thickened walls of cells undergoing PCD, and those vesicles and materials reaching the pollen tube after passing through the extracellular matrix were taken into the tube by endocytosis. These results show that in PCD of nucellar cells, intracellular materials are degraded in amyloplasts and vacuoles, and some of the degraded material is supplied to the pollen tube by vesicular transport to support tube growth.

  20. Outcrossing and paternity analysis of Pinus densiflora (Japanese red pine) by microsatellite polymorphism.

    Science.gov (United States)

    Lian, C; Miwa, M; Hogetsu, T

    2001-07-01

    This study employed microsatellite loci to analyse outcrossing rate and pollen dispersal in Japanese red pine (Pinus densiflora) in an isolated stand. The average offspring outcrossing rate for 29 cones was 0.955. Significant differences in outcrossing rates between offspring groups on individual branches that extended in different directions at different heights were not detected. Male parents of 874 offspring collected from the maternal tree were assessed by exclusion using polymorphisms at three microsatellite loci. Paternity analysis indicated that at least 31% of the offspring were fertilized by pollen from trees outside the stand. The average distance of pollen migration within the study stand was 68 m, with a maximum value of 325 m. There was excess mating with nearby P. densiflora trees, of which only a few were predominant pollen donors. In addition, a weakly directional bias in P. densiflora pollination was also detected in the study stand, suggesting that female strobili on a branch of the maternal tree were more easily fertilized by pollen from trees in that direction.