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Sample records for brasilense tarrand krieg

  1. Krieg und Literatur

    Directory of Open Access Journals (Sweden)

    Elfi N. Theis

    2007-03-01

    Full Text Available Schreiben gegen Krieg und Gewalt heißt der Band 19 der Schriften-Reihe des Erich-Maria-Remarque-Archivs, in dem es um Ingeborg Bachmann und die deutschsprachige Literatur 1945-1980 geht. Der Band enthält die Beiträge zu einem Symposion, das am 14.-15. Januar 2005 an der Universität Nottingham stattgefunden hat. Im Mittelpunkt stand die Frage, welche Strategien im Umgang mit Nationalsozialismus, Holocaust, zweitem Weltkrieg, Kaltem Krieg oder Vietnamkrieg und auch dem deutschen Kolonialismus bei Bachmann und anderen deutschsprachigen Autoren zu finden sind. Anlass zur Tagung war die in Wien und Salzburg konzipierte Ausstellung Schreiben gegen den Krieg: Ingeborg Bachmann, 1926-1973. In insgesamt dreizehn Beiträgen wird im vorliegenden Band die literarische Auseinandersetzung mit dem Thema Gewalt und Krieg beleuchtet.

  2. Biosíntesis de ácido antranílico y ácido indolacético a partir de triptófano en una cepa de Azospirillum brasilense nativa de Tamaulipas, México

    OpenAIRE

    J. L. Hernández-Mendoza; J. D. Quiroz-Velásquez; V. R. Moreno-Medina; N. Mayek-Pérez

    2008-01-01

    La bacteria Azospirillum brasilense [Tarrand et al., 1979] metaboliza el triptófano y produce diversos metabolitos; entre ellos, el ácido indolacético. En este trabajo se utilizó la cepa CBG 497 de A. brasilense aislada de maíz para identificar los metabolitos producidos a partir de triptófano en laboratorio. Entre los metabolitos identificados destacan los ácidos antranílico e indolacético. El ácido antranílico es un compuesto intermediario en la síntesis y degrada...

  3. Mathematik und Krieg

    DEFF Research Database (Denmark)

    Booss-Bavnbek, Bernhelm

    2004-01-01

    Dieser Beitrag ist eine leicht aktualisierte Zusammenfassung der Ergebnisse gemeinsamer Arbeit mit dem Roskilder Mathematikhistoriker Jens Høyrup und einer internationalen Konferenz zum gleichen Thema, die in Karlskrona im August 2002 aus Anlass des 60. Geburtstags des Verfassers stattfand. Die T...... Themen “Mathematik im Zweiten Weltkrieg”, “Mathematik als Teil der modernen Kriegführung”, “Mathematisierung des Krieges” und “Mathematik und Vernunft” werden behandelt....

  4. Der Erste Schleswigsche Krieg 1848 -1851. Vorgeschichte, Verlauf und Folgen

    DEFF Research Database (Denmark)

    Christensen, Jens Ole; Adriansen, Inge

    Dieses Heft über den Ersten Schleswigschen Krieg 1848-1851 bringt eine leicht zugängliche Übersicht über die Vorgeschichte und den Verlauf des Krieges sowie seine weitreichenden Auswirkungen in vielen Bereichen.......Dieses Heft über den Ersten Schleswigschen Krieg 1848-1851 bringt eine leicht zugängliche Übersicht über die Vorgeschichte und den Verlauf des Krieges sowie seine weitreichenden Auswirkungen in vielen Bereichen....

  5. Der Zweite Schleswigsche Krieg 1864. Vorgeschichte, Verlauf und Folgen

    DEFF Research Database (Denmark)

    Christensen, Jens Ole; Adriansen, Inge

    Dieses Buch über den Zweiten Schleswigschen Krieg bietet eine gut lesbare Übersicht zur Vorgeschichte und zum Verlauf des Krieges sowie zu dessen tiefgreifenden und langandauernden Folgen auf vielen Gebieten.......Dieses Buch über den Zweiten Schleswigschen Krieg bietet eine gut lesbare Übersicht zur Vorgeschichte und zum Verlauf des Krieges sowie zu dessen tiefgreifenden und langandauernden Folgen auf vielen Gebieten....

  6. Ovita ovia Zürn - "Zürn's Krieg"

    NARCIS (Netherlands)

    Gewald, J.B.; Förster, von L.; Henrichsen, D.; Bollig, M.

    2004-01-01

    Am 12. Januar 1904 brach in Namibia ein Krieg zwischen der deutschen Kolonialverwaltung und grossen Teilen der lokalen Bevölkerung aus - ein Krieg, der später als Herero-Krieg in die Annalen der Kolonialgeschichte eingehen sollte. Dieses Kapitel versucht in dichter Beschreibung der Ereignisse

  7. Krieg und Literatur War and Literature

    Directory of Open Access Journals (Sweden)

    Elfi N. Theis

    2007-03-01

    Full Text Available Schreiben gegen Krieg und Gewalt heißt der Band 19 der Schriften-Reihe des Erich-Maria-Remarque-Archivs, in dem es um Ingeborg Bachmann und die deutschsprachige Literatur 1945-1980 geht. Der Band enthält die Beiträge zu einem Symposion, das am 14.-15. Januar 2005 an der Universität Nottingham stattgefunden hat. Im Mittelpunkt stand die Frage, welche Strategien im Umgang mit Nationalsozialismus, Holocaust, zweitem Weltkrieg, Kaltem Krieg oder Vietnamkrieg und auch dem deutschen Kolonialismus bei Bachmann und anderen deutschsprachigen Autoren zu finden sind. Anlass zur Tagung war die in Wien und Salzburg konzipierte Ausstellung Schreiben gegen den Krieg: Ingeborg Bachmann, 1926-1973. In insgesamt dreizehn Beiträgen wird im vorliegenden Band die literarische Auseinandersetzung mit dem Thema Gewalt und Krieg beleuchtet.Volume 19 of the series published by the Erich-Maria-Remarque Archive is entitled “Writing against War and Violence” (“Schreiben gegen Krieg und Gewalt” and approaches Ingeborg Bachmann and German language literature from 1945 to 1980. The volume contains contributions based on a symposium that took place at the University of Nottingham on January 14-15, 2005. Central to the symposium was the question as to which strategies Bachmann and other German language authors utilized in their approach to National Socialism, the Holocaust, the Second World War, the Cold War, or the Vietnam War, as well as German colonialism. The impetus for the conference was the exhibition Writing Against the War: Ingeborg Bachmann, 1926-1973 conceived in Vienna and Salzburg. In the volume at hand, thirteen contributions in total illuminate literary confrontations with the themes of war and violence.

  8. Der Krieg als eine besondere Form des Konflikts

    Directory of Open Access Journals (Sweden)

    Sebastian Schneider

    2011-12-01

    Full Text Available Comme le montrent les débats politiques et scientifiques actuels, la notion de guerre ne décrit pas uniquement une condition, mais a des implications normatives larges. Dans les situations de guerre, l’interdiction générale de tuer est en partie levée ou limitée. Afin d’éviter toute instrumentalisation politique et arbitraire de la notion de guerre, il faut repenser les critères de distinction entre la notion de guerre et les autres formes de conflit. Cet article se propose d’évoquer les réflexions philosophiques ainsi que politiques sur le thème de la guerre et critique le manque de réflexion sur la distinction entre la notion de guerre et les autres formes de conflits.Wie sich an aktuellen politischen und wissenschaftlichen Debatten zeigen lässt, ist der Begriff des Krieges nicht nur eine reine Zustandsbeschreibung, sondern hat auch weitreichende normative Implikationen. Im Krieg ist das ansonsten generelle Tötungsverbot partiell aufgehoben oder eingeschränkt. Um den Begriff nicht einer willkürlichen politischen Instrumentalisierung zu übergeben, muss über Kriterien zur Abgrenzung von Krieg und anderen Formen des Konflikts nachgedacht werden. Der Artikel wirft einen Blick auf die Beschäftigung der Philosophie und Politikwissenschaft mit dem Thema Krieg und kritisiert die mangelnde Abgrenzung des Krieges zu anderen Konfliktformen.

  9. R ad, D er Heilige Krieg im alten Israel. Gotting

    African Journals Online (AJOL)

    Test

    G erhard von. R ad, D er Heilige Krieg im alten Israel. Gotting en, Vandenhoeck & Ruprecht, 1952, 2 Aufl., 84 S. Hierdie geskrif van die bekende Heidelberger Outestamentikus is die uitwerking van 'n voordrag in 1949 op die somersamekoms van die. Britse Society for Old Testament Study gehou en aan daardie belang-.

  10. 10 Jahre danach: Der Irak-Krieg 2003 und das (Medien- Schlachtfeld II

    Directory of Open Access Journals (Sweden)

    Karl H. Stingeder

    2013-06-01

    Full Text Available Hinsichtlich der Informationsstrategie der US-Administration von George W. Bush und des US-–Militärs im Zuge des Irak-Krieges 2003, lässt sich eine gut geplante und organisierte PR feststellen. Die eingebetteten Journalisten waren dabei ein wichtiges Mittel zum Zweck. Sie sorgten für eine Bilderhoheit im Krieg. Auch waren sie als Quelle für „unverfängliche Berichte“ geplant. – Eine vordergründig „neutrale Berichterstattung“, die von negativen Aspekten des Krieges ablenken sollte. Dabei war die Medienlandschaft der USA nach dem 11. September 2001 durch weitgehende Konformität geprägt. Kritik am Präsidenten oder an der Regierung wurde häufig als unpatriotisch gewertet. Somit wurde ein idealer Nährboden für Propaganda geschaffen.Der Irak-Krieg 2003 stellt einen politisch gewollten und inszenierten Krieg dar. Im Zuge der Anschuldigungen gegen Saddam Hussein sowie infolge der medialen Inszenierung des Kriegs kristallisierte sich heraus, dass Politik und Militär den Sprung ins moderne Informationszeitalter geschafft haben. Beim modernen Informationskrieg findet der Krieg auf einer weiteren, virtuellen Ebene statt. Information, Nichtinformation und Desinformation sind die effizienten Waffen dieses Krieges. Dabei wird die Desinformation der Öffentlichkeit bewusst in Kauf genommen und JournalistInnen werden als Vermittler politischer Propaganda instrumentalisiert.

  11. Desinformation und Destabilisierung – Russlands unerklärter Krieg

    OpenAIRE

    Heinke, Daniel H.

    2016-01-01

    „Wir sind im Krieg mit Russland.“ Diese gleichermaßen lakonische wie wuchtige Bewertung des Journalisten Jan-Philipp Hein lässt den Leser unwillkürlich zusammenzucken, klingt sie doch wie ein aus der Zeit gefallenes Zitat einer längst überwundenen Ost-West-Konfrontation. Zudem erscheint sie verstörend irreal, da die sich in den Vordergrund drängenden aktuellen sicherheitspolitischen Herausforderungen wie islamistischer Terrorismus, anhaltend große (und vielfach unkontrollierte) Flüchtlingsstr...

  12. [Sascha Möbius. Das Gedähtnis der Reichsstadt. Unruhen und Kriege in der lübeckischen Chronistik und Kriege und Erinnerungskultur des späten mittelalters und der frühen Neuzeit

    Index Scriptorium Estoniae

    Hormuth, Dennis

    2012-01-01

    Arvustus: Sascha Möbius. Das Gedähtnis der Reichsstadt. Unruhen und Kriege in der lübeckischen Chronistik und Kriege und Erinnerungskultur des späten mittelalters und der frühen Neuzeit. (Göttingen, 2011)

  13. Polymers selection for a liquid inoculant of Azospirillum brasilense ...

    African Journals Online (AJOL)

    In this study, we used a method of accelerated degradation to select a polymer and a concentration to maintain cell stability of a liquid inoculant based on the strain C16 Azospirillum brasilense. A screening at 45°C was made to compare the protectant effect of five polymers on the viability of the strain (p/v): carrageenan ...

  14. Review: Larissa Förster, Postkoloniale Erinnerungslandschaften. Wie Deutsche und Herero in Namibia des Kriegs von 1904 gedenken (2010 Buchbesprechung: Larissa Förster, Postkoloniale Erinnerungslandschaften. Wie Deutsche und Herero in Namibia des Kriegs von 1904 gedenken (2010

    Directory of Open Access Journals (Sweden)

    Reinhart Kößler

    2010-01-01

    Full Text Available Review of the Monograph: Larissa Förster (2010, Postkoloniale Erinnerungslandschaften. Wie Deutsche und Herero in Namibia des Kriegs von 1904 gedenken, Frankfurt am Main & New York: Campus, ISBN 978-3-593-39160-1, 391 pages. Besprechung der Monographie: Larissa Förster (2010, Postkoloniale Erinnerungslandschaften. Wie Deutsche und Herero in Namibia des Kriegs von 1904 gedenken, Frankfurt am Main & New York: Campus, ISBN 978-3-593-39160-1, 391 Seiten.

  15. [Jörg Zägel (in Zusammenarbeit mit Reiner Steinweg). Vergangenheitsdiskurse in der Ostseeregion. Bd. 1. Auseinandersetzungen in den nordischen Staaten über Krieg. Völkermord, Diktatur, Besatzung und Vertreibung; B. 2 : Die Sicht auf Krieg, Diktat

    Index Scriptorium Estoniae

    Hecker-Stampehl, Jan

    2011-01-01

    Arvustus : Jörg Zägel (in Zusammenarbeit mit Reiner Steinweg). Vergangenheitsdiskurse in der Ostseeregion. Bd. 1. Auseinandersetzungen in den nordischen Staaten über Krieg. Völkermord, Diktatur, Besatzung und Vertreibung. Berlin : LIT Verlag, 2007. (Kieler Schriften zur Friedenswissenschaft. 14); Bd. 2 : Die Sicht auf Krieg, Diktatur, Völkermord, Besatsung und Vertreibung in Russland, Polen und den baltischen Staaten. Berlin: Lit Verlag, 2007. (Kieler Schriften zur Friedenswissenschaft. 15)

  16. Azospirillum brasilense siderophores with antifungal activity against Colletotrichum acutatum.

    Science.gov (United States)

    Tortora, María L; Díaz-Ricci, Juan C; Pedraza, Raúl O

    2011-04-01

    Anthracnose, caused by the fungus Colletotrichum acutatum is one of the most important diseases in strawberry crop. Due to environmental pollution and resistance produced by chemical fungicides, nowadays biological control is considered a good alternative for crop protection. Among biocontrol agents, there are plant growth-promoting bacteria, such as members of the genus Azospirillum. In this work, we demonstrate that under iron limiting conditions different strains of A. brasilense produce siderophores, exhibiting different yields and rates of production according to their origin. Chemical assays revealed that strains REC2 and REC3 secrete catechol type siderophores, including salicylic acid, detected by thin layer chromatography coupled with fluorescence spectroscopy and gas chromatography-mass spectrometry analysis. Siderophores produced by them showed in vitro antifungal activity against C. acutatum M11. Furthermore, this latter coincided with results obtained from phytopathological tests performed in planta, where a reduction of anthracnose symptoms on strawberry plants previously inoculated with A. brasilense was observed. These outcomes suggest that some strains of A. brasilense could act as biocontrol agent preventing anthracnose disease in strawberry.

  17. Directed mutagenesis affects recombination in Azospirillum brasilense nif genes

    Directory of Open Access Journals (Sweden)

    C.P. Nunes

    2000-12-01

    Full Text Available In order to improve the gene transfer/mutagenesis system for Azospirillum brasilense, gene-cartridge mutagenesis was used to replace the nifD gene with the Tn5 kanamycin resistance gene. The construct was transferred to A. brasilense by electrotransformation. Of the 12 colonies isolated using the suicide plasmid pSUP202 as vector, only four did not show vector integration into the chromosome. Nevertheless, all 12 colonies were deficient in acetylene reduction, indicating an Nif- phenotype. Four Nif- mutants were analyzed by Southern blot, using six different probes spanning the nif and Km r genes and the plasmid vector. Apparently, several recombination events occurred in the mutant genomes, probably caused mainly by gene disruption owing to the mutagenesis technique used: resistance gene-cartridge mutagenesis combined with electrotransformation.Com o objetivo de melhorar os sistemas de transferência gênica e mutagênese para Azospirillum brasilense, a técnica de mutagênese através do uso de um gene marcador ("gene-cartridge mutagenesis" foi utilizada para substituir a região genômica de A. brasilense correspondente ao gene nifD por um segmento de DNA do transposon Tn5 contendo o gene que confere resistência ao antibiótico canamicina. A construção foi transferida para a linhagem de A. brasilense por eletrotransformação. Doze colônias transformantes foram isoladas com o plasmídeo suicida pSUP202 servindo como vetor. Dessas, somente quatro não possuíam o vetor integrado no cromossomo da bactéria. Independentemente da integração ou não do vetor, as 12 colônias foram deficientes na redução do gás acetileno, evidenciando o fenótipo Nif -. Quatro mutantes Nif - foram analisados através da técnica de Southern blot, utilizando-se seis diferentes fragmentos contendo genes nif, de resistência à canamicina e do vetor como sondas. Os resultados sugerem a ocorrência de eventos recombinacionais variados no genoma dos mutantes. A

  18. Eumenes II. als persona non grata: Pergamon, Rom und der 3. Makedonische Krieg

    OpenAIRE

    Christoph Kenkel

    2012-01-01

    Christoph Kenkel treads on difficult terrain with his Bachelor Thesis Eumenes II als persona non grata: Pergamon, Rom und der 3. Makedonische Krieg ( Eumenes II as persona non grata: Pergamon, Rome and the Third Macedonian War), but manages to develop his own convincing interpretation of a research topic subjected to strong discourse.Starting with the deprecatory demeanor of the Roman Senate towards Eumenes II Sotor (197-159) in 167 B.C., the author begins a search for this unusual rejection,...

  19. Purification and binding analysis of the nitrogen fixation regulatory NifA protein from Azospirillum brasilense

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    L.M.P. Passaglia

    1998-11-01

    Full Text Available NifA protein activates transcription of nitrogen fixation operons by the alternative sigma54 holoenzyme form of RNA polymerase. This protein binds to a well-defined upstream activator sequence (UAS located at the -200/-100 position of nif promoters with the consensus motif TGT-N10-ACA. NifA of Azospirillum brasilense was purified in the form of a glutathione-S-transferase (GST-NifA fusion protein and proteolytic release of GST yielded inactive and partially soluble NifA. However, the purified NifA was able to induce the production of specific anti-A. brasilense NifA-antiserum that recognized NifA from A. brasilense but not from K. pneumoniae. Both GST-NifA and NifA expressed from the E. coli tac promoter are able to activate transcription from the nifHDK promoter but only in an A. brasilense background. In order to investigate the mechanism that regulates NifA binding capacity we have used E. coli total protein extracts expressing A. brasilense nifA in mobility shift assays. DNA fragments carrying the two overlapping, wild-type or mutated UAS motifs present in the nifH promoter region revealed a retarded band of related size. These data show that the binding activity present in the C-terminal domain of A. brasilense NifA protein is still functional even in the presence of oxygen.

  20. Und es gibt sie doch: Frauen, die Krieg und Frieden theoretisieren

    Directory of Open Access Journals (Sweden)

    Ellen Krause

    2001-11-01

    Full Text Available Sandra Hedinger liest Bertha von Suttner, Rosa Luxemburg, Hannah Arendt, Betty Reardon, Ann J. Tickner und Jean Bethke Elshtain auf Aussagen zum Geschlechterverhältnis, zu Kriegsursachen und zur Kriegsüberwindung. Dabei zeigt sie den engen Zusammenhang dieser Pole im Denken der behandelten Theoretikerinnen. Hedingers Buch ist eine überzeugende Aufforderung an die wissenschaftliche Beschäftigung mit den Internationalen Beziehungen, den herkömmlichen, männlichen Textkorpus um bisher ignorierte Überlegungen und Autorinnen zu erweitern.

  1. Rezension zu: Judith Butler: Krieg und Affekt. Zürich u.a.: diaphanes 2009.

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    Rolf Löchel

    2009-10-01

    Full Text Available Seit einigen Jahren nimmt die gender-Theoretikerin Judith Butler in zunehmendem Maße auch Themen der politischen Ethik in den Blick. Ihre in den drei Texten des schmalen Bandes vorgetragene pazifistische Ideen und ihre Überlegungen angesichts des Irak-Kriegs, aber auch zur Burka oder der universellen Geltung von Menschenrechten sind allerdings nicht immer so originell oder gar überzeugend wie Butlers bahnbrechenden gender-theoretischen Entwürfe während der ersten Hälfte der 1990er Jahre.

  2. Fritz Haber und der „Krieg der Chemiker“: Leben und Werk eines umstrittenen Wissenschaftlers

    OpenAIRE

    Friedrich, B

    2015-01-01

    Der Physikochemiker Fritz Haber (1868-1934) ging für sein Ammoniak-Syntheseverfahren zur Herstellung von künstlichem Dünger („Brot aus der Luft“) in die Geschichte ein. Das Haber-Bosch-Verfahren wurde aber ebenso wichtig für die Erzeugung von Schießpulver. Während des Ersten Weltkriegs entwickelte Haber Giftgaswaffen für die deutschen Truppen und wurde deswegen auch als „Vater des Gaskrieges“ bekannt. Der Erste Weltkrieg wird auch als „Krieg der Chemiker“ bezeichnet. 1933 weigerte sich Haber,...

  3. Prokofieff: Krieg und Frieden (Sinfonische Suite), Die Verlobung im Kloster (Sommernacht-Suite), Russische Overtüre. Philharmonia Orchestra, Neeme Järvi / G. W.

    Index Scriptorium Estoniae

    G. W.

    1993-01-01

    Uuest heliplaadist "Prokofieff: Krieg und Frieden (Sinfonische Suite), Die Verlobung im Kloster (Sommernacht-Suite), Russische Overtüre. Philharmonia Orchestra, Neeme Järvi. (AD: 1991). Chandos/Koch CD 9096

  4. Economic analysis of corn inoculated with Azospirillum brasilense associated with nitrogen sources and doses

    Directory of Open Access Journals (Sweden)

    Fernando Shintate Galindo

    2017-08-01

    Full Text Available Azospirillum brasilense is a bacterium known for its biological nitrogen fixation (BNF in corn crops. However, there is a lack of comprehensive research defining how much mineral N should be applied to maximize the efficiency of BNF and attain high, economically sustainable yields. Moreover, it would be interesting to investigate whether adding urea with NBPT urease inhibitor might increase BNF in grasses. Therefore, the aim of this study was to determine the effect of inoculation with Azospirillum brasilense associated with N sources and doses in a Cerrado biome soil by evaluating the grain yield of irrigated corn in economic terms. The experiment was conducted in Selvíria, MS, Brazil under a no-till system on a Latossolo Vermelho distrófico (Oxisol. The experiment was set up as a randomized block design with four replications in a 2 × 5 × 2 factorial arrangement consisting of two sources of N (urea and urea with NBPT urease enzyme inhibitor and five N doses applied as top-dressing (0, 50, 100, 150, and 200 kg ha-1, with and without the inoculation of seeds with A. brasilense. Inoculation with Azospirillum brasilense makes corn growth much more profitable, irrespective of the dose and source of N. Addition of 200 kg ha-1 N in the form of conventional urea coupled with inoculation with Azospirillum brasilense increases grain yield; however, the highest economic return is obtained with N applied at 100 kg ha-1 with conventional urea and inoculation.

  5. Insa Eschebach, Regina Mühlhäuser (Hg.: Krieg und Geschlecht. Sexuelle Gewalt im Krieg und Sex-Zwangsarbeit in NS-Konzentrationslagern. Berlin: Metropol Verlag 2008.

    Directory of Open Access Journals (Sweden)

    Veronika Springmann

    2009-11-01

    Full Text Available In den letzten Jahren sind die Auseinandersetzung mit den Akteuren und Funktionen von Gewalt und auch die Forschungen und Deutungsweisen zu sexueller Gewalt forciert worden. Der Tagungsband versammelt neue Forschungsbeiträge und Interpretationsmuster zu sexueller Gewalt im Krieg und Sex-Zwangsarbeit in NS-Konzentrationslagern, um erstens den aktuellen Stand der Forschung aufzuzeigen und zweitens nach künftigen Perspektiven zu fragen. Die Aufsätze informieren hervorragend über aktuelle Fragen und Debatten zu diesen Themen.In recent years, the conflict with protagonists of and functions of violence has accelerated, as has research on and interpretation of sexual violence. The conference volume collects new contributions to research on and interpretive patterns of sexual violence in war and in sexual forced labor in National Socialist concentration camps, in order first to depict the current state of research and second to look for future perspectives. The brilliant essays inform readers on the current questions and debates on these topics.

  6. Der allerschönste Krieg : [aus "Kõige ilusam sõda"¡ ("Der allerschönste Krieg")] : [luuletused] / Asko Künnap ; tlk. Irja Grönholm ja Gisbert Jänicke

    Index Scriptorium Estoniae

    Künnap, Asko

    2006-01-01

    Sisu: Der allerschönste Krieg : Ich suchte mein nichtgelebtes Leben ; Wie fühlt sich's Seite an Seite ; So toll, dass wir kommen konnten ; Wie kommt man lebend aus Schweden zurück? ; Im Schlaf vereint ; Ruft die Spinnen - dass sie die Nacht weben ; Du hältst die Welt von mir fern ; Ruder mich zur Insel ; In den Augen des Mädchens aus dem Norden ; Der allerschönste Krieg ; Der Teufel im Sakko zu sechshundert Kronen. Orig.: Kõige ilusam sõda : Otsisin oma elamata jäänud elu ; Kuidas on kõrvuti olla ; Nii tore, et tulla saime ; Kuidas tulla elusana tagasi Rootsist? ; Unes ühes ; Kutsuge ämblikud - las punuvad öö ; Sina hoiad maailma minust ära ; Sõua mind saareni ; Ühe põhjamaa tüdruku silmades ; Kõige ilusam sõda ; Kurat kuuesajakroonises pintsakus

  7. 10 Jahre danach: Der Irak-Krieg 2003 und das (Medien- Schlachtfeld I - Der Medien-Golfkrieg im Spannungsfeld zwischen PR und Propaganda

    Directory of Open Access Journals (Sweden)

    Karl Stinged

    2013-03-01

    Full Text Available Hinsichtlich der Informationsstrategie der US-Administration von George W. Bush und des US-Militärs im Zuge des Irak-Krieges 2003, lässt sich eine gut geplante und organisierte PR feststellen. Die eingebetteten Journalisten waren dabei ein wichtiges Mittel zum Zweck. Sie sorgten für eine Bilderhoheit im Krieg. Auch waren sie als Quelle für "unverfängliche Berichte" geplant. Eine vordergründig "neutrale Berichterstattung", die von negativen Aspekten des Krieges ablenken sollte.Die Medienlandschaft der USA nach dem 11. September 2001 war durch eine weitgehende Konformität geprägt. Kritik am Präsidenten oder an der Regierung wurde häufig als unpatriotisch gewertet. Somit wurde ein idealer Nährboden für Propaganda geschaffen. Propaganda ist systematische, persuasive Public Relations, die mit manipulativen Mitteln arbeitet. Die eigentliche politische Agenda, welche von den Propagandisten verfolgt wird, soll verschleiert werden.Der Irak-Krieg 2003 ist ein politisch gewollter und inszenierter Krieg. Im Zuge der Anschuldigungen gegen Saddam Hussein sowie in Folge der medialen Inszenierung des Kriegs, kristallisierte sich heraus, dass Politik und Militär den Sprung ins moderne Informationszeitalter geschafft haben. Beim modernen Informationskrieg findet der Krieg auf einer weiteren, virtuellen Ebene statt. Information, Nichtinformation und Desinformation sind die effizienten Waffen dieses Krieges. Dabei wird die Desinformation der Öffentlichkeit bewusst in Kauf genommen. JournalistInnen werden dabei als Vermittler politischer Propaganda instrumentalisiert.Die amerikanische Bevölkerung wurde durch die gezielte Manipulation der Medien zu einer Pro-Kriegs-Haltung verleitet. Die Behauptungen, die im Zuge der Anschuldigungen gegen das irakische Regime, aufgestellt wurden, erwiesen sich größtenteils als falsch und manipulativ. Die im Zuge der Vorbereitungen sowie während des Irak-Kriegs 2003 verwendete PR-Strategien können daher als

  8. Identification and characterization of the two-component NtrY/NtrX regulatory system in Azospirillum brasilense

    Directory of Open Access Journals (Sweden)

    M.L. Ishida

    2002-06-01

    Full Text Available Two Azospirillum brasilense open reading frames (ORFs exhibited homology with the two-component NtrY/NtrX regulatory system from Azorhizobium caulinodans. These A. brasilense ORFs, located downstream to the nifR3ntrBC operon, were isolated, sequenced and characterized. The present study suggests that ORF1 and ORF2 correspond to the A. brasilense ntrY and ntrX genes, respectively. The amino acid sequences of A. brasilense NtrY and NtrX proteins showed high similarity to sensor/kinase and regulatory proteins, respectively. Analysis of lacZ transcriptional fusions by the ß-galactosidase assay in Escherichia coli ntrC mutants showed that the NtrY/NtrX proteins failed to activate transcription of the nifA promoter of A. brasilense. The ntrYX operon complemented a nifR3ntrBC deletion mutant of A. brasilense for nitrate-dependent growth, suggesting a possible cross-talk between the NtrY/X and NtrB/C sensor/regulator pairs. Our data support the existence of another two-component regulatory system in A. brasilense, the NtrY/NtrX system, probably involved in the regulation of nitrate assimilation.

  9. Effect of seed inoculation with Azospirillum brasilense and nitrogen fertilization rates on maize plant yield and silage quality

    Directory of Open Access Journals (Sweden)

    Fernando Reimann Skonieski

    Full Text Available ABSTRACT The objective of this study was to determine the effect of Azospirillum brasilense inoculation and different nitrogen (N rates applied as topdressing on the productivity of a maize crop and the nutritional value of maize silage. Two experiments were conducted in the 2012/2013 and 2013/2014 harvests. Treatments were distributed in a randomized block design in a factorial arrangement, which consisted of two maize hybrids (AS 1572 and Defender coupled with nitrogen rates (0, 60, 120, 240, and 480 kg ha-1, inoculated or uninoculated with A. brasilense. Inoculated seeds were treated with the A. brasilense strains Ab-V5 and Ab-V6. Inoculation with A. brasilense showed interaction with the hybrids, agricultural years, and nitrogen rates for the maize plant yield. In the 2012/2013 agricultural year, inoculation increased the AS 1572 hybrid silage yield by 6.16% and, in the 2013/2014 harvest, A. brasilense inoculation produced an increase of 16.15% for the Defender hybrid. Nitrogen fertilization applied at 0, 60, and 120 kg ha-1 N benefited the plants inoculated with A. brasilense. The statistical equations revealed that N rates between 0 and 184 kg ha-1 in A. brasilense inoculated plants raised the plant productivity for silage when compared with the control plants. Regarding the nutritional value of the silage, inoculation with A. brasilense increased the ether extract levels and total digestible nutrients and reduced the amount of acid detergent fiber. For all this, positive results with inoculation for silage yield are dependent on nitrogen fertilization rate. Inoculation with A. brasilense can promote changes in the maize silage quality, but with obtained results it is not possible to definitely conclude upon nutritive value of maize silage.

  10. Kloster Eberbach im Krieg – Bedeutende Verluste der Abtei während des Bauernkriegs und des Dreißigjährigen Kriegs

    Directory of Open Access Journals (Sweden)

    Markus Studer

    2012-05-01

    Full Text Available The seminar paper „KlosterEberbach im Krieg. Bedeutende Verluste der Abtei während des Bauernkriegs und des Dreißigjährigen Kriegs“ (Eberbach Abbey at War: Significant losses during the Peasant’s War and the Thirty-Year War by Markus Studer deals with the history of Eberbach Abbey in the Rheingau area and focusses on the role of the abbey within the religious order of the Cistercians.After the abbey was founded in 1136 near to the River Kisselbach, it managed to build up a productive economic network and could acquire many estates. With an extensive group of donorsand a close relationship with the archbishop of Mainz, the abbey could establish a worldy position of power,which in turn favoured the expansion of the Cistercians.A peasant revolt struck the monastery in 1525 and its legendary „big barrel“ was plundered. This act was considered a grave humiliation and led to an identity crisis. Traces of this crisis can actually be found in the historical sources. After this event, the economy of Eberbach was badly damaged and in the course of the Thirty Years War it became utterly shattered. Positioned on the bottom of a valley and protected by only one defensive wall, the abbey was easy prey for the Swedish and Hessian troops. As a consequence, many monks fled into exile. Not only were precious works of art and religious items stolen, but also the vast library (numbering 754 works was plundered. The location of the majority of the stolen books remains uncertain to this day.

  11. FTIR spectroscopic study of biofilms formed by the rhizobacterium Azospirillum brasilense Sp245 and its mutant Azospirillum brasilense Sp245.1610

    Science.gov (United States)

    Tugarova, Anna V.; Scheludko, Andrei V.; Dyatlova, Yulia A.; Filip'echeva, Yulia A.; Kamnev, Alexander A.

    2017-07-01

    Biofilms are spatially and metabolically structured communities of microorganisms, representing a mode of their existence which is ubiquitous in nature, with cells localised within an extracellular biopolymeric matrix, attached to each other, at an interface. For plant-growth-promoting rhizobacteria (PGPR), the formation of biofilms is of special importance due to their primary localisation at the surface of plant root systems. In this work, FTIR spectroscopy was used, for the first time for bacteria of the genus Azospirillum, to comparatively study 6-day-mature biofilms formed on the surface of ZnSe discs by the rhizobacterium Azospirillum brasilense Sp245 and its mutant A. brasilense Sp245.1610. The mutant strain, having an Omegon Km insertion in the gene of lipid metabolism fabG1 on the plasmid AZOBR_p1, as compared to the wild-type strain Sp245 (see http://dx.doi.org/10.1134/S1022795413110112)

  12. Repressor mutant forms of the Azospirillum brasilense NtrC protein.

    Science.gov (United States)

    Huergo, Luciano F; Assumpção, Marcelo C; Souza, Emanuel M; Steffens, M Berenice R; Yates, M Geoffrey; Chubatsu, Leda S; Pedrosa, Fábio O

    2004-10-01

    The Azospirillum brasilense mutant strains FP8 and FP9, after treatment with nitrosoguanidine, showed a null Nif phenotype and were unable to use nitrate as their sole nitrogen source. Sequencing of the ntrC genes revealed single nucleotide mutations in the NtrC nucleotide-binding site. The phenotypes of these strains are discussed in relation to their genotypes.

  13. Rhizobial Inoculation, Alone or Coinoculated with Azospirillum brasilense, Promotes Growth of Wetland Rice

    Directory of Open Access Journals (Sweden)

    Leandro Hahn

    Full Text Available ABSTRACT Rhizobia and associative bacteria promote growth in rice plants (Oryza sativa L. through a series of mechanisms, but most studies on inoculation have been performed based on inoculation with these bacteria in a separate or singular manner. The objective of this study was to assess the efficiency of single/isolated inoculation and inoculation combined with symbiotic rhizobia from forage legume and with Azospirillum brasilense on promoting growth and the root colonization process in wetland rice. Two rhizobia among four isolates from a greenhouse and a laboratory experiment were selected that efficiently promoted seed germination and rice plant growth in a sterilized substrate and in soil. The two most efficient isolates (UFRGS Vp16 and UFRGS Lc348 were inoculated alone or in combination with a commercial product containing A. brasilense in two field experiments using two wetland rice cultivars over two growing seasons. In the field experiments, these isolates coinoculated with A. brasilense promoted larger increases in the agronomic variables of wetland rice compared to the control without inoculation. Confocal laser microscopy confirmed the presence of inoculated bacteria tagged with gfp (UFRGS Vp16, UFRGS Lc348, and A. brasilense colonizing the root surface of the rice seedlings, mainly in the root hairs and lateral roots.

  14. CRECIMIENTO DE Cedrela odorata L. BIOFERTILIZADA CON Rhizophagus intraradices y Azospirillum brasilense EN VIVERO

    Directory of Open Access Journals (Sweden)

    Juan F. Aguirre-Medina

    2014-01-01

    Full Text Available Cedrela odorata L. es una especie que se distingue por la calidad de su madera. El cedro esta - blecido en campo debe adaptarse a cambios en disponibilidad de recursos; la sobrevivivencia es mayor cuando el sistema radical se asocia con microorganismos que mejoran la nutrición y crecimiento. El objetivo del presente trabajo fue evaluar el efecto de Rhizophagus intraradi - ces y Azospirillum brasilense sobre el crecimiento de C. odorata y la capacidad de absorción de nitrógeno (N y fósforo (P. Las semillas de cedro se sembraron en macetas de acuerdo con los siguientes trata - mientos: testigo, fertilización 15 N -15 P -15 K , R. intraradices , A. brasilense , R. intraradices + A. brasilense , y R. intraradices + A. brasilense + fertilización 15 N -15 P -15 K . Las variables morfológicas y fisiológicas de rendimiento y la colonización radical se registraron cada 28 días, y los contenidos de N y P, al final del experimento (140 días. En general, R. intraradices promovió mayor crecimiento vegetal ( P ≤ 0.05 de los componentes morfológicos y fisiológicos del rendimiento. La altura máxima (97 cm se registró en las plantas con R. intraradices + A. brasilense + fertilización 15 N -15 P -15 K , representando 40 % más altura que el testigo. El N y P incrementaron con la inoculación de los microorganismos solos y combinados.

  15. Comparison of Two Cellulomonas Strains and Their Interaction with Azospirillum brasilense in Degradation of Wheat Straw and Associated Nitrogen Fixation

    OpenAIRE

    Halsall, Dorothy M.; Gibson, Alan H.

    1986-01-01

    A mutant strain of Cellulomonas sp. CS1-17 was compared with Cellulomonas gelida 2480 as the cellulolytic component of a mixed culture which was responsible for the breakdown of wheat straw to support asymbiotic nitrogen fixation by Azospirillum brasilense Sp7 (ATCC 29145). Cellulomonas sp. strain CSI-17 was more efficient than was C. gelida in cellulose breakdown at lower oxygen concentrations and, in mixed culture with A. brasilense, it supported higher nitrogenase activity (C2H2 reduction)...

  16. Judith Butler: Krieg und Affekt. Zürich u.a.: diaphanes 2009.

    Directory of Open Access Journals (Sweden)

    Rolf Löchel

    2009-11-01

    Full Text Available Seit einigen Jahren nimmt die gender-Theoretikerin Judith Butler in zunehmendem Maße auch Themen der politischen Ethik in den Blick. Ihre in den drei Texten des schmalen Bandes vorgetragene pazifistische Ideen und ihre Überlegungen angesichts des Irak-Kriegs, aber auch zur Burka oder der universellen Geltung von Menschenrechten sind allerdings nicht immer so originell oder gar überzeugend wie Butlers bahnbrechenden gender-theoretischen Entwürfe während der ersten Hälfte der 1990er Jahre.For a number of years the gender theorist Judith Butler has been increasingly concerned with topics related to political ethics. The three texts in the slim volume contain her ideas of pacifism and her thoughts prompted by the Iraq war as well as on burkas or the universal import of human rights. These texts are, however, not always as original or even convincing as Butler’s groundbreaking gender theoretical models of the first half of the 1990s.

  17. Eumenes II. als persona non grata: Pergamon, Rom und der 3. Makedonische Krieg

    Directory of Open Access Journals (Sweden)

    Christoph Kenkel

    2012-11-01

    Full Text Available Christoph Kenkel treads on difficult terrain with his Bachelor Thesis Eumenes II als persona non grata: Pergamon, Rom und der 3. Makedonische Krieg ( Eumenes II as persona non grata: Pergamon, Rome and the Third Macedonian War, but manages to develop his own convincing interpretation of a research topic subjected to strong discourse.Starting with the deprecatory demeanor of the Roman Senate towards Eumenes II Sotor (197-159 in 167 B.C., the author begins a search for this unusual rejection, facing the challenge of a confusing array of sources and the many diverging opinions of researchers. Further complications arise from the complex political situation at the eve of the Third Macedonian War that raises a variety of new issues.Nevertheless Kenkel manages to see through the machinations and rumors of Roman politics by applying logical thought, and explains plausibly why Eumenes, who unjustly got caught in the crossfire, has always been an ally of Rome, despite of many contemporary rumors and keen allegations.

  18. Cold War in Southern Africa Kalter Krieg im Südlichen Afrika

    Directory of Open Access Journals (Sweden)

    Matthew Graham

    2010-01-01

    Full Text Available Review Article: Cold War in Southern Africa Gary Baines, Peter Vale (eds. (2008, Beyond the Border War: New Perspectives on Southern Africa’s Late-Cold War Conflicts, Pretoria: Unisa Press, ISBN 978 1 86888 456 8, xix + 342 pp. Sue Onslow (ed. (2009, Cold War in Southern Africa: White Power, Black Liberation, Abingdon: Routledge, ISBN 978 0 415 47420 7, 253 pp. Vladimir Shubin (2008, The Hot “Cold War”: The USSR in Southern Africa, London: Pluto Press, ISBN 978 0745324722, 320 pp. Review Article: Kalter Krieg im Südlichen Afrika Gary Baines, Peter Vale (eds. (2008, Beyond the Border War: New Perspectives on Southern Africa’s Late-Cold War Conflicts, Pretoria: Unisa Press, ISBN 978 1 86888 456 8, xix + 342 S. Sue Onslow (ed. (2009, Cold War in Southern Africa: White Power, Black Liberation, Abingdon: Routledge, ISBN 978 0 415 47420 7, 253 S. Vladimir Shubin (2008, The Hot “Cold War”: The USSR in Southern Africa, London: Pluto Press, ISBN 978 0745324722, 320 S.

  19. Israelkritik und Antisemitismus? Eine vergleichende Analyse der deutschen Presseberichterstattung über 2. Intifada und Gaza-Krieg

    Directory of Open Access Journals (Sweden)

    Markus Maurer

    2011-10-01

    Full Text Available Die deutsche Medienberichterstattung über den Nahost-Konflikt wurde verschiedentlich dahingehend kritisiert, einen anti-israelische Bias zu haben, der die alten, gebräuchlichen Vorurteile und Stereotype gegenüber Juden stärke und die Anschuldigung unterstütze, die Juden würden die Tragik des Holocaust missbrauchen um die israelische Palästina-Politik zu rechtfertigen: Insbesondere während des Gaza-Krieges sei Israel als Aggressor dargestellt, der palästinensische Terrorismus dagegen heruntergespielt worden. Bisher gibt es kaum systematische Forschung zu dieser Frage. Die vorliegende Studie soll diese Lücke zu füllen helfen, indem sie eine vergleichende Inhaltsanalyse der Berichterstattung über die 2. Intifada und den Gaza-Krieg in Die Welt, Frankfurter Allgemeine Zeitung, Süddeutsche Zeitung, Frankfurter Rundschau und Tageszeitung vornimmt. Eine repräsentative Stichprobe von 396 Artikeln aus diesen Zeitungen, die das gesamte politische Spektrum von rechts nach links abdecken, wurde entlang der drei Dimensionen (1 Darstellung des Verhaltens der Konfliktparteien, (2 Bewertung ihrer Intentionen und Handlungen, und (3 Interpunktion des Konfliktes & Darstellung seiner Opfer analysiert. Die Ergebnisse der Studie zeigen, dass die Darstellung beider Konflikte weit differenzierter war als von ihren Kritikern angenommen. Auch während des Gaza-Krieges hat die deutsche Qualitätspresse ihr Bestes getan, sich nicht auf die Seite der Palästinenser zu schlagen. Falls sie tatsächlich zur Stärkung anti-israelischer oder sogar antisemitischer Einstellungen beigetragen hat, so war dies eher der Erzeugung von Reaktanz geschuldet als einem anti-israelischen Bias der Berichterstattung.

  20. Interaction of Azospirillum brasilense and Glomus intrarradix in Sugar Cane Roots.

    Science.gov (United States)

    Bellone, Carlos H; de Bellone Silvia, Carrizo

    2012-03-01

    Fifteen-day-old variety NA 56-79 sugar cane seedlings were inoculated with Azospirillum brasilense and Glomus intrarradix. This article aims at examining changes in sugar cane root seedlings inoculated with Glomus intrarradix and Azospirillum brasilense, the increase in microbial biomass and the acetylene reduction process as well. The internal root colonization was studied 20 days after inoculation using scanning and a transmission electron microscope. Both microorganisms entered the sugar cane root through the emergent lateral roots. The microorganisms were capable of coexisting both intra and intercellularly, producing changes in the cell wall, thus allowing colonization and interaction between the organisms. These changes increased the number of microorganisms inside the root as well as acetylene nitrogen reduction. Sugar cane plant biomass increased with joint-inoculation. The number of endophytic microorganisms and nitrogen fixing activity increased when they were colonized by Azospirillum and Glomus together.

  1. Tryptophan, thiamine and indole-3-acetic acid exchange between Chlorella sorokiniana and the plant growth-promoting bacterium Azospirillum brasilense.

    Science.gov (United States)

    Palacios, Oskar A; Gomez-Anduro, Gracia; Bashan, Yoav; de-Bashan, Luz E

    2016-06-01

    During synthetic mutualistic interactions between the microalga Chlorella sorokiniana and the plant growth-promoting bacterium (PGPB) Azospirillum brasilense, mutual exchange of resources involved in producing and releasing the phytohormone indole-3-acetic acid (IAA) by the bacterium, using tryptophan and thiamine released by the microalga, were measured. Although increased activities of tryptophan synthase in C. sorokiniana and indole pyruvate decarboxylase (IPDC) in A. brasilense were observed, we could not detect tryptophan or IAA in the culture medium when both organisms were co-immobilized. This indicates that no extra tryptophan or IAA is produced, apart from the quantities required to sustain the interaction. Over-expression of the ipdC gene occurs at different incubation times: after 48 h, when A. brasilense was immobilized alone and grown in exudates of C. sorokiniana and at 96 h, when A. brasilense was co-immobilized with the microalga. When A. brasilense was cultured in exudates of C. sorokiniana, increased expression of the ipdC gene, corresponding increase in activity of IPDC encoded by the ipdC gene, and increase in IAA production were measured during the first 48 h of incubation. IAA production and release by A. brasilense was found only when tryptophan and thiamine were present in a synthetic growth medium (SGM). The absence of thiamine in SGM yielded no detectable IAA. In summary, this study demonstrates that C. sorokiniana can exude sufficient tryptophan and thiamine to allow IAA production by a PGPB during their interaction. Thiamine is essential for IAA production by A. brasilense and these three metabolites are part of a communication between the two microorganisms. © FEMS 2016. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.

  2. Growth and yield of corn hybrids in response to association with Azospirillum brasilense and nitrogen fertilization

    Directory of Open Access Journals (Sweden)

    Deniele Marini

    2015-02-01

    Full Text Available There is a growing interest in optimizing the positive effects of the association between Azospirillum bacteria and corn crop in order to reduce the use of nitrogen fertilizers. This study aimed to evaluate the inoculation efficiency of an A. brasilense-based commercial product in association with different rates of nitrogen fertilization in two corn genotypes. The experiment was arranged in a 2 x 2 x 5 factorial randomized block design, with four replications. The treatments consisted of two corn hybrids (30F53 and CD386; with and without inoculation with a commercial product based on A. brasilense and five nitrogen rates (0, 40, 80, 120 and 160 kg ha-1. The variables plant height, basal stem diameter, leaf area, shoot dry matter, leaf nitrogen content, length and diameter of the cob, weight of 100 grains and grain yield were evaluated. Inoculation with A. brasilense provided increases of 11 and 12% in leaf area and shoot dry matter, respectively. There were differences in the response of the corn hybrids for most variables and the increase in nitrogen supply provided increments in the growth and yield of corn.

  3. Heiliger Krieg? Die Kriegserzählungen des Alten Israel in den Büchern Josua und Deuteronomium im Spiegel der politischen Reden George W. Bushs nach dem 11. September 2001 und aktueller hermeneutischer Debatten

    OpenAIRE

    Münch-Wirtz, Julia

    2010-01-01

    Spätestens seit dem 11. September 2001 hat der Terminus Heiliger Krieg Hochkonjunktur. Sowohl die immer wiederkehrende Gewalt als auch die signifikante Verbindung von Religion und Politik veranlassen vor allem die alttestamentliche Theologie dazu, diese Thematik zu einem wesentlichen Bestandteil der wissenschaftlichen Reflexion zu machen. In diesem Kontext stellt sich die Frage, was es überhaupt bedeutet, von einem Heiligen Krieg zu sprechen, und aufgrund welcher Konvention oder Tradition...

  4. Toxicidade de herbicidas utilizados na cultura da cana-de-açúcar à bactéria diazotrófica Azospirillum brasilense Toxicity of herbicides applied on sugarcane to the diazotrophic bacterium Azospirillum brasilense

    Directory of Open Access Journals (Sweden)

    S.O Procópio

    2011-01-01

    Full Text Available Objetivou-se neste trabalho identificar herbicidas utilizados na cultura da cana-de-açúcar que não alteram o crescimento ou a capacidade de fixação biológica de nitrogênio (FBN da bactéria diazotrófica Azospirillum brasi lense. Dezoito herbicidas - paraquat, ametryn, amicarbazone, diuron, metribuzin, [hexazinone + diuron], [hexazinone + clomazone], clomazone, isoxaflutole, sulfentrazone, oxyfluorfen, imazapic, imazapyr, [trifloxysulfuron-sodium + ametryn], S-metolachlor, glyphosate, MSMA e 2,4-D - foram testados em suas doses comerciais quanto ao impacto sobre o crescimento da bactéria em meio líquido DIGs. As variáveis capacidade de suporte de crescimento (carrying capacity do meio de cultura, duração da fase lag e tempo de geração de A. brasilense foram calculadas a partir de dados de densidade ótica obtidos, em intervalos regulares, durante a incubação de culturas por 55 h. O impacto dos herbicidas na atividade da nitrogenase de A. brasilense foi avaliado em meio semissólido NFb, sem N, pela técnica da atividade de redução do acetileno (ARA. Os efeitos dos herbicidas sobre as variáveis de crescimento e ARA foram comparados ao controle pelo teste de Dunnett. Paraquat, oxyfluorfen, [trifloxysulfuron-sodium + ametryn] e glyphosate reduziram a capacidade do meio DIGs em suportar o crescimento de A. brasilense. Esse efeito foi associado ao aumento da duração da fase lag e do tempo de geração para [trifloxysulfuron-sodium + ametryn] e ao aumento no tempo de geração para glyphosate. MSMA, paraquat e amicarbazone reduzem a FBN in vitro de A. brasilense, porém essa redução é mais severa na presença do paraquat. Os demais herbicidas não alteram o crescimento e a FBN de A. brasilense.The objective of this work was to identify the herbicides applied on sugarcane that do not affect the growth nor the process of biological nitrogen fixation (BNF of the diazotrophic bacterium Azospirillum brasilense. Commercial doses of

  5. Enhanced activity of ADP glucose pyrophosphorylase and formation of starch induced by Azospirillum brasilense in Chlorella vulgaris.

    Science.gov (United States)

    Choix, Francisco J; Bashan, Yoav; Mendoza, Alberto; de-Bashan, Luz E

    2014-05-10

    ADP-glucose pyrophosphorylase (AGPase) regulates starch biosynthesis in higher plants and microalgae. This study measured the effect of the bacterium Azospirillum brasilense on AGPase activity in the freshwater microalga Chlorella vulgaris and formation of starch. This was done by immobilizing both microorganisms in alginate beads, either replete with or deprived of nitrogen or phosphorus and all under heterotrophic conditions, using d-glucose or Na-acetate as the carbon source. AGPase activity during the first 72h of incubation was higher in C. vulgaris when immobilized with A. brasilense. This happened simultaneously with higher starch accumulation and higher carbon uptake by the microalgae. Either carbon source had similar effects on enzyme activity and starch accumulation. Starvation either by N or P had the same pattern on AGPase activity and starch accumulation. Under replete conditions, the population of C. vulgaris immobilized alone was higher than when immobilized together, but under starvation conditions A. brasilense induced a larger population of C. vulgaris. In summary, adding A. brasilense enhanced AGPase activity, starch formation, and mitigation of stress in C. vulgaris. Copyright © 2014 Elsevier B.V. All rights reserved.

  6. Metabolic profiling of two maize (Zea mays L.) inbred lines inoculated with the nitrogen fixing plant-interacting bacteria Herbaspirillum seropedicae and Azospirillum brasilense

    National Research Council Canada - National Science Library

    Liziane Cristina Brusamarello-Santos; Françoise Gilard; Lenaïg Brulé; Isabelle Quilleré; Benjamin Gourion; Pascal Ratet; Emanuel Maltempi de Souza; Peter J Lea; Bertrand Hirel

    2017-01-01

    .... Two established model strains of Nif+ diazotrophic bacteria, Herbaspirillum seropedicae and Azospirillum brasilense and their Nif- couterparts defficient in nitrogenase activity, were used to evaluate the impact of the bacterial...

  7. Residual effect of nitrogen fertilization and Azospirillum brasilense inoculation in the maize cultureEfeito residual da adubação nitrogenada e inoculação de Azospirillum brasilense na cultura do milho

    Directory of Open Access Journals (Sweden)

    Jackson Huzar Novakowiski

    2011-12-01

    Full Text Available The diazotrophic bacteria Azospirillum brasilense is an organism able to fix nitrogen of atmosphere and produce plant hormones. Nevertheless, there is lack of information with regard to use in field conditions, especially in production systems that have presence of animals in a determined year period. The objective of paper was to evaluate the association of the nitrogen residual effect of fertilization in pasture winter and the inoculation with A. brasilense in the maize culture. Were carried two experiments in Guarapuava (PR in season 2008/09 with randomized block design with split plots in three replication. The main parcel consisted of application 5 levels of N (0, 75, 150, 225 e 300 kg ha-1 in pasture of black oat and ryegrass occupied by bovines and 4 treatments in the maize (T1 = control; T2 = inoculation of A. brasilense; T3 = 75 kg ha-1 of N; T4 = 150 kg ha-1 of N. Were evaluated: plants population, ears per plant, productivity, mass thousand grains, damage grains, row per ear and grains per row. There was residual effect of nitrogen applied in the pasture on maize culture. The mass thousand grains and the number of rows per ear of maize presented quadratic response for increase of nitrogen level in the pasture. The inoculation of A. brasilense provided higher productivity than control same with increase the nitrogen level applied in the pasture, with quadratic response in the experiment 1 and linear in the experiment 2.A bactéria diazotrófica Azospirillum brasilense é um organismo capaz de fixar nitrogênio da atmosfera e produzir hormônios vegetais. No entanto, há carência de informações a respeito de seu uso em condições de campo, sobretudo em sistemas de produção que tem a presença de animais em um determinado período do ano. O objetivo do trabalho foi avaliar a associação do efeito residual da adubação nitrogenada na pastagem de inverno e a inoculação de A. brasilense na cultura do milho. Foram conduzidos dois

  8. Crecimiento de Azospirillum brasilense en presencia de disacáridos: sacarosa y lactosa

    OpenAIRE

    Yudyt Díaz-Saez; Manuel Díaz-de los Ríos; Mario Alberto-Casas; Arianna Nuñez-Caraballo; Marlene Martínez-Mora

    2013-01-01

    La obtención de biopolímeros a partir de fermentaciones bacterianas requiere de la selección adecuada de las condiciones del proceso. Se evaluó el crecimiento de la cepa Azospirillum brasilense 8-INICA en medio líquido suplementado con disacáridos. Las mediciones se realizaron a las 23 h de incubación con la determinación de la densidad óptica, la concentración de azúcares reductores y concentración de carbohidratos totales, así como la determinación del pH del sobrenadante. Se confirmó el má...

  9. Krieg der Geschlechter – Politik der Geschlechter The War of the Sexes—The Politics of the Sexes

    Directory of Open Access Journals (Sweden)

    Ruth Hagengruber

    2005-03-01

    Full Text Available Während der Topos vom Krieg der Geschlechter Jahrhunderte alt ist, ist die gendersensible Bewertung politischer Mittel ein junges Forschungsgebiet. Das Buch ist als Handbuch politikwissenschaftlicher Grundbegriffe konzipiert. In den Beiträgen österreichischer, schweizerischer und deutscher Theoretikerinnen werden Schlüsselbegriffe der Politikwissenschaft diskutiert, historische Entwicklungen nachgezeichnet und methodische Ansätze erläutert. Die klare Organisation der Texte, die durch Textpfade und Begriffserklärungen im Glossar ergänzt werden, sowie eine umfassende Bibliographie bieten eine hervorragende Diskussions- und Forschungsgrundlage über die politische Disziplin hinaus.While the theme of the war of the sexes is centuries old, the gender sensitive review of political resources is a new area of research. The collection sees itself as a handbook for basic vocabulary of political science. In the contributions from Austrian, Swiss, and German theoreticians, the key terms of political science are discussed, historical developments sketched, and methodological approaches elucidated. The clear organization of the texts, which are supplemented by text paths and explanation of terms in the glossary, and the comprehensive bibliography offer a wonderful foundation for discussion and research that reaches well beyond the political discipline.

  10. Involvement of indole-3-acetic acid produced by Azospirillum brasilense in accumulating intracellular ammonium in Chlorella vulgaris.

    Science.gov (United States)

    Meza, Beatriz; de-Bashan, Luz E; Bashan, Yoav

    2015-01-01

    Accumulation of intracellular ammonium and activities of the enzymes glutamine synthetase (GS) and glutamate dehydrogenase (GDH) were measured when the microalgae Chlorella vulgaris was immobilized in alginate with either of two wild type strains of Azospirillum brasilense or their corresponding indole-3-acetic acid (IAA)-attenuated mutants. After 48 h of immobilization, both wild types induced higher levels of intracellular ammonium in the microalgae than their respective mutants; the more IAA produced, the higher the intracellular ammonium accumulated. Accumulation of intracellular ammonium in the cells of C. vulgaris followed application of four levels of exogenous IAA reported for A. brasilense and its IAA-attenuated mutants, which had a similar pattern for the first 24 h. This effect was transient and disappeared after 48 h of incubation. Immobilization of C. vulgaris with any bacteria strain induced higher GS activity. The bacterial strains also had GS activity, comparable to the activity detected in C. vulgaris, but weaker than when immobilized with the bacteria. When net activity was calculated, the wild type always induced higher GS activity than IAA-attenuated mutants. GDH activity in most microalgae/bacteria interactions resembled GS activity. When complementing IAA-attenuated mutants with exogenous IAA, GS activity in co-immobilized cultures matched those of the wild type A. brasilense immobilized with the microalga. Similarity occurred when the net GS activity was measured, and was higher with greater quantities of exogenous IAA. It is proposed that IAA produced by A. brasilense is involved in ammonium uptake and later assimilation by C. vulgaris. Copyright © 2014 Institut Pasteur. Published by Elsevier Masson SAS. All rights reserved.

  11. Fluorescein Isothiocyanate-Labeled Lectin Analysis of the Surface of the Nitrogen-Fixing Bacterium Azospirillum brasilense by Flow Cytometry

    OpenAIRE

    Yagoda-Shagam, Janet; Barton, Larry L.; Reed, William P.; Chiovetti, Robert

    1988-01-01

    The cell surface of Azospirillum brasilense was probed by using fluorescein isothiocyanate (FITC)-labeled lectins, with binding determined by fluorescence-activated flow cytometry. Cells from nitrogen-fixing or ammonium-assimilating cultures reacted similarly to FITC-labeled lectins, with lectin binding in the following order: Griffonia simplicifolia II agglutinin > Griffonia simplicifolia I agglutinin > Triticum vulgaris agglutinin > Glycine max agglutinin > Canavalia ensiformis agglutinin >...

  12. Comparison of Two Cellulomonas Strains and Their Interaction with Azospirillum brasilense in Degradation of Wheat Straw and Associated Nitrogen Fixation

    Science.gov (United States)

    Halsall, Dorothy M.; Gibson, Alan H.

    1986-01-01

    A mutant strain of Cellulomonas sp. CS1-17 was compared with Cellulomonas gelida 2480 as the cellulolytic component of a mixed culture which was responsible for the breakdown of wheat straw to support asymbiotic nitrogen fixation by Azospirillum brasilense Sp7 (ATCC 29145). Cellulomonas sp. strain CSI-17 was more efficient than was C. gelida in cellulose breakdown at lower oxygen concentrations and, in mixed culture with A. brasilense, it supported higher nitrogenase activity (C2H2 reduction) and nitrogen fixation with straw as the carbon source. Based on gravimetric determinations of straw breakdown and total N determinations, the efficiency of nitrogen fixation was 72 and 63 mg of N per g of straw utilized for the mixtures containing Cellulomonas sp. and C. gelida, respectively. Both Cellulomonas spp. and Azospirillum spp. exhibited a wide range of pH tolerance. When introduced into sterilized soil, the Cellulomonas sp.-Azospirillum brasilense association was more effective in nitrogen fixation at a pH of 7.0 than at the native soil pH (5.6). This was also true of the indigenous diazotrophic microflora of this soil. The potential implications of this work to the field situation are discussed. PMID:16347043

  13. Residual phosphate fertilization and Azospirillum brasilense in the common bean in succession to maize intercropped with Marandu grass

    Directory of Open Access Journals (Sweden)

    Lourdes Dickmann

    Full Text Available ABSTRACT One of the alternatives for achieving sustainable agriculture and a reduction in production costs, especially with phosphate fertilisers, is to inoculate seeds with bacteria of the genus Azospirillum. The aim of this study therefore, was to evaluate residual phosphate fertilisation and Azospirillum brasilense, together with the contribution of straw from maize intercropped with Marandu grass, on leaf nutritional content, yield components and winter bean yield. The experiment was carried out on the Teaching and Research Farm, of the School of Engineering at UNESP, located in Selvíria in the State of Mato Grosso do Sul, in a typic clayey dystrophic Red Latosol. The experimental design was of randomised blocks with four replications in a 5 x 2 factorial scheme. The treatments consisted of beans sown on straw from maize intercropped with Marandu grass on areas that had received five levels of P2O5 in the form of MAP, applied during an initial cultivation of black oats (0, 30, 60, 120 and 240 kg ha-1, both with and without inoculation of the oat and maize which preceded the beans with Azospirillum brasilense. Leaf nutrient content, leaf chlorophyll index (ICF, yield components and bean productivity were all evaluated. Inoculation with Azospirillum brasilense of the black oat and maize seeds improved the nutritional status of the plants, but had a negative effect on grain yield. Fertilisation of the oat crop with phosphorus had a positive residual effect on the beans, with increases in yield components and grain yield.

  14. Accumulation of fatty acids in Chlorella vulgaris under heterotrophic conditions in relation to activity of acetyl-CoAcarboxylase, temperature, and co-immobilization with Azospirillum brasilense [corrected].

    Science.gov (United States)

    Leyva, Luis A; Bashan, Yoav; Mendoza, Alberto; de-Bashan, Luz E

    2014-10-01

    The relation between fatty acid accumulation, activity of acetyl-CoA carboxylase (ACC), and consequently lipid accumulation was studied in the microalgae Chlorella vulgaris co-immobilized with the plant growth-promoting bacterium Azospirillum brasilense under dark heterotrophic conditions with Na acetate as a carbon source. In C. vulgaris immobilized alone, cultivation experiments for 6 days showed that ACC activity is directly related to fatty acid accumulation, especially in the last 3 days. In co-immobilization experiments, A. brasilense exerted a significant positive effect over ACC activity, increased the quantity in all nine main fatty acids, increased total lipid accumulation in C. vulgaris, and mitigated negative effects of nonoptimal temperature for growth. No correlation between ACC activity and lipid accumulation in the cells was established for three different temperatures. This study demonstrated that the interaction between A. brasilense and C. vulgaris has a significant effect on fatty acid and lipid accumulation in the microalgae.

  15. Accumulation fatty acids of in Chlorella vulgaris under heterotrophic conditions in relation to activity of acetyl-CoA carboxylase, temperature, and co-immobilization with Azospirillum brasilense

    Science.gov (United States)

    Leyva, Luis A.; Bashan, Yoav; Mendoza, Alberto; de-Bashan, Luz E.

    2014-10-01

    The relation between fatty acid accumulation, activity of acetyl-CoA carboxylase (ACC), and consequently lipid accumulation was studied in the microalgae Chlorella vulgaris co-immobilized with the plant growth-promoting bacterium Azospirillum brasilense under dark heterotrophic conditions with Na acetate as a carbon source. In C. vulgaris immobilized alone, cultivation experiments for 6 days showed that ACC activity is directly related to fatty acid accumulation, especially in the last 3 days. In co-immobilization experiments, A. brasilense exerted a significant positive effect over ACC activity, increased the quantity in all nine main fatty acids, increased total lipid accumulation in C. vulgaris, and mitigated negative effects of nonoptimal temperature for growth. No correlation between ACC activity and lipid accumulation in the cells was established for three different temperatures. This study demonstrated that the interaction between A. brasilense and C. vulgaris has a significant effect on fatty acid and lipid accumulation in the microalgae.

  16. Enzyme Production and Nitrogen Fixation by Free, Immobilized and Coimmobilized Inoculants of Trichoderma harzianum and Azospirillum brasilense and Their Possible Role in Growth Promotion of Tomato

    Directory of Open Access Journals (Sweden)

    Momein H. El-Katatny

    2010-01-01

    Full Text Available A plant growth-promoting rhizobacterium (Azospirillum brasilense strain Az and a biocontrol fungus (Trichoderma harzianum strain T24 have been evaluated for their individual and combined production of hydrolytic enzymes, nitrogen fixation and their possible role in growth promotion of tomato seedlings. The studied organisms were inoculated as free or calcium alginate-encapsulated cells. All freshly prepared macrobeads showed high encapsulation capacity (EC/% of inocula compared with dry macrobeads. Results of enzyme production did not exhibit consistent pattern of the effect of encapsulation process on enzyme production. Beads entrapping bacterial and/or fungal cells were used successfully in 3 repeated cycles in the presence of fresh sterile culture medium in each growth cycle. Enzyme production by immobilized bacterial and/or fungal cells increased as the growth cycles were repeated. Co-culturing of A. brasilense with T. harzianum (free or immobilized in semisolid nitrogen deficient medium (N-free medium enabled A. brasilense to fix nitrogen on pectin, chitin and carboxymethyl cellulose. The activity of nitrogen fixation by A. brasilense in the case of single and combined cultures with Trichoderma (using dry encapsulated beads into the sterile soil increased with the addition of carbon source. Most of inoculations with free or alginate macrobead formulations of T. harzianum and/or A. brasilense showed significant increase in the growth parameters of tomato seedlings. The root system grew more profusely in the case of all seeds treated with A. brasilense. The growth parameters of Az/T24-treated seeds using dry coimmobilized macrobeads were higher than those of the untreated control. Moreover, the effect was improved significantly in soil enriched with different C sources. Enhanced tomato seedling growth after the co-inoculation could be due to the synergistic effect of both Trichoderma and Azospirillum. Finally, co-inoculation with Azospirillum

  17. Dual RNA-seq transcriptional analysis of wheat roots colonized by Azospirillum brasilense reveals up-regulation of nutrient acquisition and cell cycle genes.

    Science.gov (United States)

    Camilios-Neto, Doumit; Bonato, Paloma; Wassem, Roseli; Tadra-Sfeir, Michelle Z; Brusamarello-Santos, Liziane C C; Valdameri, Glaucio; Donatti, Lucélia; Faoro, Helisson; Weiss, Vinicius A; Chubatsu, Leda S; Pedrosa, Fábio O; Souza, Emanuel M

    2014-05-16

    The rapid growth of the world's population demands an increase in food production that no longer can be reached by increasing amounts of nitrogenous fertilizers. Plant growth promoting bacteria (PGPB) might be an alternative to increase nitrogenous use efficiency (NUE) in important crops such wheat. Azospirillum brasilense is one of the most promising PGPB and wheat roots colonized by A. brasilense is a good model to investigate the molecular basis of plant-PGPB interaction including improvement in plant-NUE promoted by PGPB. We performed a dual RNA-Seq transcriptional profiling of wheat roots colonized by A. brasilense strain FP2. cDNA libraries from biological replicates of colonized and non-inoculated wheat roots were sequenced and mapped to wheat and A. brasilense reference sequences. The unmapped reads were assembled de novo. Overall, we identified 23,215 wheat expressed ESTs and 702 A. brasilense expressed transcripts. Bacterial colonization caused changes in the expression of 776 wheat ESTs belonging to various functional categories, ranging from transport activity to biological regulation as well as defense mechanism, production of phytohormones and phytochemicals. In addition, genes encoding proteins related to bacterial chemotaxi, biofilm formation and nitrogen fixation were highly expressed in the sub-set of A. brasilense expressed genes. PGPB colonization enhanced the expression of plant genes related to nutrient up-take, nitrogen assimilation, DNA replication and regulation of cell division, which is consistent with a higher proportion of colonized root cells in the S-phase. Our data support the use of PGPB as an alternative to improve nutrient acquisition in important crops such as wheat, enhancing plant productivity and sustainability.

  18. Cellular responses during morphological transformation in Azospirillum brasilense and Its flcA knockout mutant.

    Directory of Open Access Journals (Sweden)

    Xingsheng Hou

    Full Text Available FlcA is a response regulator controlling flocculation and the morphological transformation of Azospirillum cells from vegetative to cyst-like forms. To understand the cellular responses of Azospirillum to conditions that cause morphological transformation, proteins differentially expressed under flocculation conditions in A. brasilense Sp7 and its flcA knockout mutant were investigated. Comparison of 2-DE protein profiles of wild-type (Sp7 and a flcA deletion mutant (Sp7-flcAΔ revealed a total of 33 differentially expressed 2-DE gel spots, with 22 of these spots confidently separated to allow protein identification. Analysis of these spots by liquid chromatography-tandem mass spectrometry (LC-MS/MS and MASCOT database searching identified 48 proteins (≥10% emPAI in each spot. The functional characteristics of these proteins included carbon metabolism (beta-ketothiolase and citrate synthase, nitrogen metabolism (Glutamine synthetase and nitric oxide synthase, stress tolerance (superoxide dismutase, Alkyl hydroperoxidase and ATP-dependent Clp protease proteolytic subunit and morphological transformation (transducer coupling protein. The observed differences between Sp7 wild-type and flcA- strains enhance our understanding of the morphological transformation process and help to explain previous phenotypical observations. This work is a step forward in connecting the Azospirillum phenome and genome.

  19. Biofertilization with Azospirillum brasilense improves in vitro culture of Handroanthus ochraceus, a forestry, ornamental and medicinal plant.

    Science.gov (United States)

    Llorente, Berta E; Alasia, María A; Larraburu, Ezequiel E

    2016-01-25

    Biofertilization with plant growth-promoting rhizobacteria is a potential alternative to plant productivity. Here, in vitro propagation of Handroanthus ochraceus (yellow lapacho), a forest crop with high economic and environmental value, was developed using the Azospirillum brasilense strains Cd and Az39 during rhizogenesis. Epicotiles of in vitro plantlets were multiplied in Woody Plant Medium (WPM). For rooting, elongated shoots were transferred to auxin-free Murashige-Skoog medium with Gamborg's vitamins and WPM, both at half salt concentration (½MSG and ½WPM), and inoculated with Cd or Az39 at the base of each shoot. Anatomical studies were performed using leaves cleared and stained with safranin for optical microscopy and leaves and roots metalized with gold-palladium for scanning electron microscopy (SEM). In ½WPM auxin-free medium, A. brasilense Cd inoculation produced 55% of rooting, increased root fresh and dry weight (45% and 77%, respectively), and led to lower stomata size and density with similar proportion of open and closed stomata. Both strains selectively increased the size or density of glandular trichomes in ½MSG. Moreover, bacteria were detected on the root surface by SEM. In conclusion, the difference in H. ochraceus response to A. brasilense inoculation depends on the strain and the plant culture media. Cd strain enhanced rooting in auxin-free ½WPM and produced plantlets with features similar to those expected in ex vitro plants. This work presents an innovative in vitro approach using beneficial plant-microorganism interaction as an ecologically compatible strategy in plant biotechnology. Copyright © 2015 Elsevier B.V. All rights reserved.

  20. Accumulation of intra-cellular polyphosphate in Chlorella vulgaris cells is related to indole-3-acetic acid produced by Azospirillum brasilense.

    Science.gov (United States)

    Meza, Beatriz; de-Bashan, Luz E; Hernandez, Juan-Pablo; Bashan, Yoav

    2015-06-01

    Accumulation of intra-cellular phosphate, as polyphosphate, was measured when the microalga Chlorella vulgaris was immobilized in alginate with either of two wild-type strains of the microalgae growth-promoting bacterium Azospirillum brasilense or their corresponding IAA-attenuated mutants. Wild type strains of A. brasilense induced higher amounts of intra-cellular phosphate in Chlorella than their respective mutants. Calculations comparing intra-cellular phosphate accumulation by culture or net accumulation by the cell and the amount of IAA that was produced by each of these strains revealed that higher IAA was linked to higher accumulations of intra-cellular phosphate. Application of four levels of exogenous IAA reported for A. brasilense and their IAA-attenuated mutants to cultures of C. vulgaris enhanced accumulation of intra-cellular phosphate; the higher the content of IAA per culture or per single cell, the higher was the amount of accumulated phosphate. When an IAA-attenuated mutant was complemented with exogenous IAA, accumulation of intra-cellular phosphate at the culture level was even higher than phosphate accumulation with the respective wild type strains. When calculating the net accumulation of intra-cellular phosphate in the complementation experiment, net intra-cellular phosphate induced by the IAA-attenuated mutant was completely restored and was similar to the wild strains. We propose that IAA produced by A. brasilense is linked to polyphosphate accumulation in C. vulgaris. Copyright © 2015 Institut Pasteur. Published by Elsevier Masson SAS. All rights reserved.

  1. Die nuklearen Anlagen von Hanford (1943-1987) Eine Fallstudie über die Schnittstellen von Physik, Biologie und die US-amerikanische Gesellschaft zur Zeit des Kalten Krieges

    Science.gov (United States)

    Macuglia, Daniele

    Die Geschichte des Kalten Krieges eröffnet viele Möglichkeiten, sich näher mit den Schnittstellen von Physik und Biologie während des 20. Jahrhunderts zu befassen. Nicht nur das Unglück in Tschernobyl aus dem Jahr 1986, auch das Beispiel der nuklearen Anlagen in Hanford in den Vereinigten Staaten zeigt die biologischen Folgen von nuklearer Physik.

  2. Effect of Azospirillum brasilense and Burkholderia unamae Bacteria on Maize Photosynthetic Activity Evaluated Using the Photoacoustic Technique

    Science.gov (United States)

    Gordillo-Delgado, F.; Marín, E.; Calderón, A.

    2016-09-01

    In this work, the photosynthetic process of maize plants ( Zea mays), which were grown using seeds inoculated with plant growth promoting bacteria Azospirillum brasilense and Burkholderia unamae, was monitored. Photothermal and photobaric signals obtained by a time-resolved photoacoustic measurement configuration were used for measuring the oxygen evolution rate in situ. A frequency-resolved configuration of the method was utilized to determine the oxygen diffusion coefficient and the thermal diffusivity of the maize leaves. The latter parameters, which can be used as indicators of the photosynthetic activity of maize, are found to vary according to the plant-microbe interaction. Treatment with plant growth promoting bacteria induced a decrease in the oxygen diffusion coefficient of about 20 %.

  3. Expression and characterization of an N-truncated form of the NifA protein of Azospirillum brasilense

    Directory of Open Access Journals (Sweden)

    C.Y. Nishikawa

    2012-02-01

    Full Text Available Azospirillum brasilense is a nitrogen-fixing bacterium associated with important agricultural crops such as rice, wheat and maize. The expression of genes responsible for nitrogen fixation (nif genes in this bacterium is dependent on the transcriptional activator NifA. This protein contains three structural domains: the N-terminal domain is responsible for the negative control by fixed nitrogen; the central domain interacts with the RNA polymerase σ54 co-factor and the C-terminal domain is involved in DNA binding. The central and C-terminal domains are linked by the interdomain linker (IDL. A conserved four-cysteine motif encompassing the end of the central domain and the IDL is probably involved in the oxygen-sensitivity of NifA. In the present study, we have expressed, purified and characterized an N-truncated form of A. brasilense NifA. The protein expression was carried out in Escherichia coli and the N-truncated NifA protein was purified by chromatography using an affinity metal-chelating resin followed by a heparin-bound resin. Protein homogeneity was determined by densitometric analysis. The N-truncated protein activated in vivo nifH::lacZ transcription regardless of fixed nitrogen concentration (absence or presence of 20 mM NH4Cl but only under low oxygen levels. On the other hand, the aerobically purified N-truncated NifA protein bound to the nifB promoter, as demonstrated by an electrophoretic mobility shift assay, implying that DNA-binding activity is not strictly controlled by oxygen levels. Our data show that, while the N-truncated NifA is inactive in vivo under aerobic conditions, it still retains DNA-binding activity, suggesting that the oxidized form of NifA bound to DNA is not competent to activate transcription.

  4. Expression and characterization of an N-truncated form of the NifA protein of Azospirillum brasilense

    Energy Technology Data Exchange (ETDEWEB)

    Nishikawa, C.Y.; Araújo, L.M.; Kadowaki, M.A.S.; Monteiro, R.A.; Steffens, M.B.R.; Pedrosa, F.O.; Souza, E.M.; Chubatsu, L.S. [Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Paraná, Curitiba, PR (Brazil)

    2012-01-27

    Azospirillum brasilense is a nitrogen-fixing bacterium associated with important agricultural crops such as rice, wheat and maize. The expression of genes responsible for nitrogen fixation (nif genes) in this bacterium is dependent on the transcriptional activator NifA. This protein contains three structural domains: the N-terminal domain is responsible for the negative control by fixed nitrogen; the central domain interacts with the RNA polymerase σ{sup 54} factor and the C-terminal domain is involved in DNA binding. The central and C-terminal domains are linked by the interdomain linker (IDL). A conserved four-cysteine motif encompassing the end of the central domain and the IDL is probably involved in the oxygen-sensitivity of NifA. In the present study, we have expressed, purified and characterized an N-truncated form of A. brasilense NifA. The protein expression was carried out in Escherichia coli and the N-truncated NifA protein was purified by chromatography using an affinity metal-chelating resin followed by a heparin-bound resin. Protein homogeneity was determined by densitometric analysis. The N-truncated protein activated in vivo nifH::lacZ transcription regardless of fixed nitrogen concentration (absence or presence of 20 mM NH{sub 4}Cl) but only under low oxygen levels. On the other hand, the aerobically purified N-truncated NifA protein bound to the nifB promoter, as demonstrated by an electrophoretic mobility shift assay, implying that DNA-binding activity is not strictly controlled by oxygen levels. Our data show that, while the N-truncated NifA is inactive in vivo under aerobic conditions, it still retains DNA-binding activity, suggesting that the oxidized form of NifA bound to DNA is not competent to activate transcription.

  5. Characterization of Cell Surface and EPS Remodeling of Azospirillum brasilense Chemotaxis-like 1 Signal Transduction Pathway mutants by Atomic Force Microscopy

    Energy Technology Data Exchange (ETDEWEB)

    Billings, Amanda N [ORNL; Siuti, Piro [ORNL; Bible, Amber [University of Tennessee, Knoxville (UTK); Alexandre, Gladys [University of Tennessee, Knoxville (UTK); Retterer, Scott T [ORNL; Doktycz, Mitchel John [ORNL; Morrell-Falvey, Jennifer L [ORNL

    2011-01-01

    To compete in complex microbial communities, bacteria must quickly sense environmental changes and adjust cellular functions for optimal growth. Chemotaxis-like signal transduction pathways are implicated in the modulation of multiple cellular responses, including motility, EPS production, and cell-to-cell interactions. Recently, the Che1 chemotaxis-like pathway from Azospirillum brasilense was shown to modulate flocculation. In A. brasilense, cell surface properties, including EPS production, are thought to play a direct role in promoting flocculation. Using atomic force microscopy (AFM), we have detected distinct changes in the surface morphology of flocculating A. brasilense Che1 mutant strains that are absent in the wild type strain. Whereas the wild type strain produces a smooth mucosal extracellular matrix, the flocculating Che1 mutant strains produce distinctive extracellular fibril structures. Further analyses using flocculation inhibition and lectin-binding assays suggest that the composition of EPS components in the extracellular matrix differs between the cheA1 and cheY1 mutants, despite an apparent similarity in the macroscopic floc structures. Collectively, these data indicate that mutations in the Che1 pathway that result in increased flocculation are correlated with distinctive changes in the extracellular matrix structure produced by the mutants, including likely changes in the EPS structure and/or composition.

  6. Nitrogen Fixation Associated with Development and Localization of Mixed Populations of Cellulomonas sp. and Azospirillum brasilense Grown on Cellulose or Wheat Straw

    Science.gov (United States)

    Halsall, Dorothy M.; Goodchild, David J.

    1986-01-01

    Mixed cultures of Cellulomonas sp. and Azospirillum brasilense were grown with straw or cellulose as the carbon source under conditions favoring the fixation of atmospheric nitrogen. Rapid increases in cell numbers, up to 109 cells per g of substrate, were evident after 4 and 5 days of incubation at 30°C for cellulose and straw, respectively. Nitrogen fixation (detected by acetylene reduction measured on parallel cultures) commenced after 2 and 4 days of incubation for straw and cellulose, respectively, and continued for the duration of the experiment. Pure cultures of Cellulomonas sp. showed an increase in cell numbers, but CO2 production was low, and acetylene reduction was not detected on either cellulose or straw. Pure cultures of A. brasilense on cellulose showed an initial increase in cell numbers (107 cells per g of substrate) over 4 days, followed by a decline presumably caused by the exhaustion of available carbon substrate. On straw, A. brasilense increased to 109 cells per g of substrate over 5 days and then declined slowly; this growth was accompanied by acetylene reduction. Scanning electron micrographs of straw incubated with a mixed culture under the above conditions for 8 days showed cells of both species in close proximity to each other. Evidence was furnished that the close spatial relationship of cells from the two species facilitated the mutually beneficial association between them and thus increased the efficiency with which the products of straw breakdown were used for nitrogen fixation. Images PMID:16347042

  7. Mutation in a gene encoding anti-sigma factor in A. brasilense confers tolerance to elevated temperature, antibacterial peptide and PEG-200 via carotenoid synthesis.

    Science.gov (United States)

    Mishra, Mukti Nath; Thirunavukkarasu, Nagarajan; Sharma, Indra Mani; Jagnnadham, Medicharla V; Tripathi, Anil K

    2008-10-01

    Azospirillum brasilense Sp7 has been shown to overproduce carotenoids if the anti-sigma factor (anti-sigma(E))-encoding gene is inactivated. The anti-sigma mutant (Car-1) of A. brasilense Sp7 was more tolerant to the stresses generated by elevated temperature (40 degrees C), PEG-200 (30 mg mL(-1)) and the antibacterial agent Polymyxin-B (PMB, 25 microg mL(-1)) but not to elevated salinity (15 mg mL(-1)). Inhibition of carotenoid synthesis by diphenylamine inhibited the ability of the mutant to tolerate all the three stresses. Out of the four stress agents, only elevated temperature and salinity induced the rpoE promoter and increased the carotenoid content in Sp7 as well as in the Car-1 mutant. Comparison of the membrane permeability of the parent and the mutant by a PMB-N-phenyl-1-naphthylamine coupled assay showed that the presence of carotenoids in the mutant reduced the permeability of their membranes. Our study indicates that the carotenoid synthesis, which is under the control of extracytoplasmic function sigma factor (sigma(E)) in A. brasilense Sp7, plays a positive role in tolerating elevated temperature, the antibacterial peptide and PEG-200.

  8. Effect of ATP and 2-oxoglutarate on the in vitro interaction between the NifA GAF domain and the GlnB protein of Azospirillum brasilense

    Energy Technology Data Exchange (ETDEWEB)

    Sotomaior, P.; Araújo, L.M.; Nishikawa, C.Y.; Huergo, L.F.; Monteiro, R.A.; Pedrosa, F.O.; Chubatsu, L.S.; Souza, E.M. [Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Paraná, Curitiba, PR (Brazil)

    2012-09-21

    Azospirillum brasilense is a diazotroph that associates with important agricultural crops and thus has potential to be a nitrogen biofertilizer. The A. brasilense transcription regulator NifA, which seems to be constitutively expressed, activates the transcription of nitrogen fixation genes. It has been suggested that the nitrogen status-signaling protein GlnB regulates NifA activity by direct interaction with the NifA N-terminal GAF domain, preventing the inhibitory effect of this domain under conditions of nitrogen fixation. In the present study, we show that an N-terminal truncated form of NifA no longer required GlnB for activity and lost regulation by ammonium. On the other hand, in trans co-expression of the N-terminal GAF domain inhibited the N-truncated protein in response to fixed nitrogen levels. We also used pull-down assays to show in vitro interaction between the purified N-terminal GAF domain of NifA and the GlnB protein. The results showed that A. brasilense GlnB interacts directly with the NifA N-terminal domain and this interaction is dependent on the presence of ATP and 2-oxoglutarate.

  9. Development of alginate-based aggregate inoculants of Methylobacterium sp. and Azospirillum brasilense tested under in vitro conditions to promote plant growth.

    Science.gov (United States)

    Joe, M M; Saravanan, V S; Islam, M R; Sa, T

    2014-02-01

    To develop co-aggregated bacterial inoculant comprising of Methylobacterium oryzae CBMB20/Methylobacterium suomiense CBMB120 strains with Azospirillum brasilense (CW903) strain and testing their efficiency as inoculants for plant growth promotion (PGP). Biofilm formation and co-aggregation efficiency was studied between A. brasilense CW903 and methylobacterial strains M. oryzae CBMB20 and M. suomiense CBMB120. Survival and release of these co-aggregated bacterial strains entrapped in alginate beads were assessed. PGP attributes of the co-aggregated bacterial inoculant were tested in tomato plants under water-stressed conditions. Results suggest that the biofilm formation efficiency of the CBMB20 and CBMB120 strains increased by 15 and 34%, respectively, when co-cultivated with CW903. Co-aggregation with CW903 enhanced the survivability of CBMB20 strain in alginate beads. Water stress index score showed least stress index in plants inoculated with CW903 and CBMB20 strains maintained as a co-aggregated inoculant. This study reports the development of co-aggregated cell inoculants containing M. oryzae CBMB20 and A. brasilense CW903 strains conferred better shelf life and stress abatement in inoculated tomato plants. These findings could be extended to other PGP bacterial species to develop multigeneric bioinoculants with multiple benefits for various crops. © 2013 The Society for Applied Microbiology.

  10. Characterization of cell surface and extracellular matrix remodeling of Azospirillum brasilense chemotaxis-like 1 signal transduction pathway mutants by atomic force microscopy

    Energy Technology Data Exchange (ETDEWEB)

    Doktycz, Mitchel John [ORNL; Morrell-Falvey, Jennifer L [ORNL

    2011-01-01

    To compete in complex microbial communities, bacteria must sense environmental changes and adjust cellular functions for optimal growth. Chemotaxis-like signal transduction pathways are implicated in the regulation of multiple behaviors in response to changes in the environment, including motility patterns, exopolysaccharide production, and cell-to-cell interactions. In Azospirillum brasilense, cell surface properties, including exopolysaccharide production, are thought to play a direct role in promoting flocculation. Recently, the Che1 chemotaxis-like pathway from A. brasilense was shown to modulate flocculation, suggesting an associated modulation of cell surface properties. Using atomic force microscopy, distinct changes in the surface morphology of flocculating A. brasilense Che1 mutant strains were detected. Whereas the wild-type strain produces a smooth mucosal extracellular matrix after 24 h, the flocculating Che1 mutant strains produce distinctive extracellular fibril structures. Further analyses using flocculation inhibition, lectin-binding assays, and comparison of lipopolysaccharides profiles suggest that the extracellular matrix differs between the cheA1 and the cheY1 mutants, despite an apparent similarity in the macroscopic floc structures. Collectively, these data indicate that disruption of the Che1 pathway is correlated with distinctive changes in the extracellular matrix, which likely result from changes in surface polysaccharides structure and/or composition.

  11. Und es gibt sie doch: Frauen, die Krieg und Frieden theoretisieren They Still Exist: Women’s Writings on Peace and War

    Directory of Open Access Journals (Sweden)

    Ellen Krause

    2001-11-01

    Full Text Available Sandra Hedinger liest Bertha von Suttner, Rosa Luxemburg, Hannah Arendt, Betty Reardon, Ann J. Tickner und Jean Bethke Elshtain auf Aussagen zum Geschlechterverhältnis, zu Kriegsursachen und zur Kriegsüberwindung. Dabei zeigt sie den engen Zusammenhang dieser Pole im Denken der behandelten Theoretikerinnen. Hedingers Buch ist eine überzeugende Aufforderung an die wissenschaftliche Beschäftigung mit den Internationalen Beziehungen, den herkömmlichen, männlichen Textkorpus um bisher ignorierte Überlegungen und Autorinnen zu erweitern.Sandra Hedinger examines texts by Bertha von Suttner, Rosa Luxemburg, Hannah Arendt, Betty Reardon, Ann J. Tickner, and Jean Bethke Elshtain for their ideas on gender relations, on the causes of war, and on ways to overcome war. Hedinger’s reading of these texts shows that the ideas of these theorists were closely linked. Hedinger’s book presents a request to the field of International Relations to open itself to new considerations and to hitherto ignored female authors.

  12. Martina Thiele, Tanja Thomas, Fabian Virchow (Hg.: Medien – Krieg – Geschlecht. Affirmationen und Irritationen sozialer Ordnungen. Wiesbaden: VS Verlag für Sozialwissenschaften 2010.

    Directory of Open Access Journals (Sweden)

    Ricarda Drüeke

    2010-11-01

    Full Text Available Die Autor/-innen des Sammelbandes geben einen Überblick über wichtige Fragestellungen zu Medien, Militär und Krieg sowie Geschlecht auf der Grundlage unterschiedlicher methodischer und theoretischer Zugänge. Ziel der Beiträge ist eine Analyse der Verschränkungen und Interaktionen zwischen diesen Bereichen aus gender- und gesellschaftstheoretischer Sicht. Im Zentrum stehen die Affirmationen und Irritationen sozialer Ordnungen, insbesondere der Geschlechterordnungen, etwa in der medialen Berichterstattung über Soldatinnen und Selbstmordattentäterinnen, in Darstellungen von Frauen und Männern in Kriegsfilmen und in Theaterstücken zur Zeit des Ersten Weltkriegs sowie historische Blicke auf frühe Kriegsberichterstatterinnen. Ergänzt werden diese Auseinandersetzungen durch die Perspektive von Journalistinnen, die aus ihrer eigenen Erfahrung berichten.Based on several different methodological and theoretical approaches, the authors of the anthology give an overview on important questions concerning media, military and war, as well as gender. An analysis of the entanglements and interactions between these domains from the viewpoint of gender and social theory is the goal of the papers. The following are in the center of the studies: affirmations and irritations of social orders, especially of gender orders, for example in media reports about female soldiers and female suicide bombers, in depictions of women and men in war movies, and in theater plays during the time of WWI, as well as historical views on early female war correspondents. These discussions are completed by the perspectives of female journalists who share their own experiences.

  13. Physiological, structural and molecular traits activated in strawberry plants after inoculation with the plant growth-promoting bacterium Azospirillum brasilense REC3.

    Science.gov (United States)

    Guerrero-Molina, M F; Lovaisa, N C; Salazar, S M; Martínez-Zamora, M G; Díaz-Ricci, J C; Pedraza, R O

    2015-05-01

    The plant growth-promoting strain REC3 of Azospirillum brasilense, isolated from strawberry roots, prompts growth promotion and systemic protection against anthracnose disease in this crop. Hence, we hypothesised that A. brasilense REC3 can induce different physiological, structural and molecular responses in strawberry plants. Therefore, the aim of this work was to study these traits activated in Azospirillum-colonised strawberry plants, which have not been assessed until now. Healthy, in vitro micropropagated plants were root-inoculated with REC3 under hydroponic conditions; root and leaf tissues were sampled at different times, and oxidative burst, phenolic compound content, malondialdehyde (MDA) concentration, callose deposition, cell wall fortification and gene expression were evaluated. Azospirillum inoculation enhanced levels of soluble phenolic compounds after 12 h post-inoculation (hpi), while amounts of cell wall bound phenolics were similar in inoculated and control plants. Other early responses activated by REC3 (at 24 hpi) were a decline of lipid peroxidation and up-regulation of strawberry genes involved in defence (FaPR1), bacterial recognition (FaFLS2) and H₂O₂ depuration (FaCAT and FaAPXc). The last may explain the apparent absence of oxidative burst in leaves after bacterial inoculation. Also, REC3 inoculation induced delayed structural responses such as callose deposition and cell wall fortification (at 72 hpi). Results showed that A. brasilense REC3 is capable of exerting beneficial effects on strawberry plants, reinforcing their physiological and cellular characteristics, which in turns contribute to improve plant performance. © 2014 German Botanical Society and The Royal Botanical Society of the Netherlands.

  14. The ntrBC genes of Azospirillum brasilense are part of a nifR3-like-ntrB-ntrC operon and are negatively regulated.

    Science.gov (United States)

    Machado, H B; Yates, M G; Funayama, S; Rigo, L U; Steffens, M B; Souza, E M; Pedrosa, F O

    1995-08-01

    A cosmid able to complement the Nif- and nitrate-dependent growth phenotypes of the Azospirillum brasilense mutant FP9 was isolated from a genomic library of the wild-type strain FP2. A 6-kb DNA region was sequenced and showed two open reading frames (ORFs) identified as the ntrB and ntrC genes. An ORF1 located upstream from the ntrB gene and coding for a 36-kDa polypeptide showed similarity to the nifR3 gene of Rhodobacter capsulatus and the ORF1 of Rhizobium leguminosarum, both located upstream from the ntrB gene in a complex operon. Two other unidentified ORFs (ORF5 and partial ORF4) coding for hydrophobic polypeptides were also observed. delta ORF1-ntrBC, ORF1, ntrB, and ntrC mutants obtained by recombination of suicide plasmids containing an insertion of a promoterless lacZ kanamycin cassette showed decreased nitrogenase activities and were unable to grow on nitrate as the sole N source. These phenotypes were restored by complementation with plasmids containing the ntrC gene. Analysis of lacZ transcriptional fusions suggested that the ORF1-ntrBC operon in Azospirillum brasilense is expressed from a promoter located upstream from the ORF1 and that it is negatively regulated by the ntrC gene product.

  15. A SAM-dependent methyltransferase cotranscribed with arsenate reductase alters resistance to peptidyl transferase center-binding antibiotics in Azospirillum brasilense Sp7.

    Science.gov (United States)

    Singh, Sudhir; Singh, Chhaya; Tripathi, Anil Kumar

    2014-05-01

    The genome of Azospirillum brasilense harbors a gene encoding S-adenosylmethionine-dependent methyltransferase, which is located downstream of an arsenate reductase gene. Both genes are cotranscribed and translationally coupled. When they were cloned and expressed individually in an arsenate-sensitive strain of Escherichia coli, arsenate reductase conferred tolerance to arsenate; however, methyltransferase failed to do so. Sequence analysis revealed that methyltransferase was more closely related to a PrmB-type N5-glutamine methyltransferase than to the arsenate detoxifying methyltransferase ArsM. Insertional inactivation of prmB gene in A. brasilense resulted in an increased sensitivity to chloramphenicol and resistance to tiamulin and clindamycin, which are known to bind at the peptidyl transferase center (PTC) in the ribosome. These observations suggested that the inability of prmB:km mutant to methylate L3 protein might alter hydrophobicity in the antibiotic-binding pocket of the PTC, which might affect the binding of chloramphenicol, clindamycin, and tiamulin differentially. This is the first report showing the role of PrmB-type N5-glutamine methyltransferases in conferring resistance to tiamulin and clindamycin in any bacterium.

  16. Genome Sequence of Azospirillum brasilense CBG497 and Comparative Analyses of Azospirillum Core and Accessory Genomes provide Insight into Niche Adaptation

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    Victor González

    2012-09-01

    Full Text Available Bacteria of the genus Azospirillum colonize roots of important cereals and grasses, and promote plant growth by several mechanisms, notably phytohormone synthesis. The genomes of several Azospirillum strains belonging to different species, isolated from various host plants and locations, were recently sequenced and published. In this study, an additional genome of an A. brasilense strain, isolated from maize grown on an alkaline soil in the northeast of Mexico, strain CBG497, was obtained. Comparative genomic analyses were performed on this new genome and three other genomes (A. brasilense Sp245, A. lipoferum 4B and Azospirillum sp. B510. The Azospirillum core genome was established and consists of 2,328 proteins, representing between 30% to 38% of the total encoded proteins within a genome. It is mainly chromosomally-encoded and contains 74% of genes of ancestral origin shared with some aquatic relatives. The non-ancestral part of the core genome is enriched in genes involved in signal transduction, in transport and in metabolism of carbohydrates and amino-acids, and in surface properties features linked to adaptation in fluctuating environments, such as soil and rhizosphere. Many genes involved in colonization of plant roots, plant-growth promotion (such as those involved in phytohormone biosynthesis, and properties involved in rhizosphere adaptation (such as catabolism of phenolic compounds, uptake of iron are restricted to a particular strain and/or species, strongly suggesting niche-specific adaptation.

  17. Chemical composition and deoxynivalenol in wheat of Central- Southern Paraná: nitrogen fertilization in top dressing associated with Azospirillum brasilense

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    Thiago Montagner Souza

    2014-02-01

    Full Text Available The impact of agricultural management practices on the quality of grain was evaluated in wheat (Triticum aestivum L. BRS Tangará from the South Central region of Paraná State (Ponta Grossa in the crop years of 2010 and 2011. The field trial was carried out in succession with soybean (2010 and corn (2011. The treatments included inoculation of seeds with Azospirillum brasilense and increasing levels of nitrogen application in top dressing (0, 30, 60, 90 and 120 kg ha-1. The experimental design was in randomized block, factorial 2 x 5 (inoculation x N levels, with four replications. The parameters evaluated were water activity, moisture, protein, and grain contamination by deoxynivalenol (DON. The data were subjected to analysis of variance, comparison of means by Tukey’s test (p<0.05 and regression for nitrogen levels. The inoculation of seeds with A. brasilense increased the protein content in grain in 2010 (+1.6%; 16.9 g 100g-1 and 2011 (+1.7%, 15.7 g 100g-1, independently of the nitrogen level (p<0.01. Levels of nitrogen in 2010 presented a positive linear response with protein content, increasing by 14.2% using non-inoculated seeds (p<0.01, R2=0.955 and 14.4% for those inoculated with A. brasilense (p<0.01, R2=0.906. However, in 2011 a quadratic response was observed between nitrogen levels and protein content (p<0.01, R2=0.99, with stabilization or reduction in protein content using high levels of nitrogen (?120 kg ha-1. The contamination by DON was greater using high levels of nitrogen (3574 ?g kg-1, 120 kg ha-1; non-inoculated seeds in 2011, with a quadratic response between nitrogen levels and contamination of grains (p<0.05, R2=0.772. Furthermore, 37.5% of the samples presented contamination by DON higher than the maximum tolerated limit established by Brazilian legislation (2000 ?g kg-1; whole-wheat grain. The data demonstrates that proper management of nitrogen enhances intrinsic effects arising from plant breeding.

  18. FTIR spectroscopic studies of selenite reduction by cells of the rhizobacterium Azospirillum brasilense Sp7 and the formation of selenium nanoparticles

    Science.gov (United States)

    Kamnev, Alexander A.; Mamchenkova, Polina V.; Dyatlova, Yulia A.; Tugarova, Anna V.

    2017-07-01

    Microbially driven reduction of selenium oxyanions to elementary selenium, often in the form of nanoparticles (NPs), is widespread in nature. A diversity of possible applications of such biogenic NPs, including those in nanobiotechnology, as well as the specificity of methodologies and mechanisms of their formation via ;green synthesis; are very attractive features justifying further studies of the processes of selenium oxyanion bioreduction and the resulting Se0 nanostructures. In this study, live biomass of the rhizobacterium Azospirillum brasilense Sp7 (harvested after the logarithmic growth phase and washed from culture medium components) was used to obtain extracellular Se NPs relatively homogeneous in size (with average diameters within 50-100 nm) in the process of selenite reduction. Both the control cultures of A. brasilense Sp7 and those incubated with SeO32- (producing Se NPs), as well as the resulting separated Se NPs were studied using Fourier transform infrared (FTIR) spectroscopy in the transmission mode (measured as dried layers on a ZnSe disc), in addition to transmission electron microscopy (TEM), to compare metabolic changes in cells and the bioorganic layers associated with the Se NPs. In the control culture (stored for 24 h in physiological saline), FTIR spectroscopic signs of poly-3-hydroxybutyrate (a 'reserve' biopolyester) were observed as a response to the lack of nutrients (trophic stress), whereas they were virtually absent in cells incubated for 24 h in physiological saline with 10 mM SeO32- (toxicity stress). FTIR spectra of Se NPs separated from bacterial cells showed bands typical of proteins, polysaccharides and lipids associated with the particles (in line with their TEM images showing a thin layer over the NPs), in addition to strong carboxylate bands, which evidently stabilise the NP structure and morphology.

  19. Adubação nitrogenada associada à inoculação com Azospirillum brasilense e Herbaspirillum seropedicae na cultura do milho

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    Janaína Dartora

    2013-10-01

    Full Text Available Bactérias dos gêneros Azospirillum e Herbaspirillum são fixadoras de nitrogênio (N atmosférico e consideradas promotoras do crescimento vegetal. Desta forma, desenvolveu-se o presente trabalho com o objetivo de avaliar o desenvolvimento e a produtividade do milho em resposta à inoculação de A. brasilense e H. seropedicae associada à adubação nitrogenada. O experimento foi conduzido em delineamento experimental de blocos ao acaso em esquema fatorial 4 x 5, com quatro repetições. Os tratamentos foram constituídos da inoculação de sementes (testemunha, estirpe de A. brasilense - Ab-V5, estirpe de H. seropedicae - SmR1 e combinação Ab-V5 + SmR1 e doses de N (0, 40, 80, 120 e 160 kg ha-1 de N. Foram avaliados: diâmetro basal do colmo, altura de planta, matéria seca de parte aérea e área foliar, nas fases vegetativa e reprodutiva, e produtividade. A adubação nitrogenada favoreceu o desenvolvimento da cultura até a máxima dose estudada. A inoculação combinada das estirpes proporcionou incrementos de 12% na matéria seca de parte aérea e 7% na produtividade em relação à testemunha indicando benefícios da combinação de bactérias diazotróficas que interagem de forma diferenciada com a planta.

  20. Eficiência da inoculação de Azospirillum brasilense associado com enraizador no crescimento e na produção de alface

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    Antonio Anicete de Lima

    2017-06-01

    Full Text Available Objetivou-se avaliar o efeito da inoculação de Azospirillum brasilense (AzBr com e sem enraizador, no crescimento, produção de biomassa e resistência a pragas da alface, cultivar Lucy Brown. O experimento foi conduzido em casa de vegetação em recipientes plásticos com capacidade de 8,0 L no espaçamento 40 x 20 cm. O delineamento experimental foi de blocos casualizados em esquema fatorial (2x3+1, sendo o primeiro fator Azospirillum com e sem enraizador de crescimento e o segundo doses de nitrogênio em cobertura, 0%, 50% e 100% da recomendação de adubação e uma testemunha com adubação convencional, com quatro repetições. Em doses médias de 50% de N em cobertura, tanto o AzBr, quanto a associação AzBr mais enraizador, apresentaram um incremento significativo na massa fresca da parte aérea e de raízes de 19,36% e 23,66%, respectivamente, em relação a dose máxima de N e a testemunha. A massa seca da parte aérea não diferiu significativamente nos diferentes tratamentos, embora tenha mostrado um pequeno aumento com as doses crescente de N. A massa seca de raízes diminuiu 29,24% no tratamento com 100% de N, quando comparado ao tratamento sem cobertura nitrogenada mais AzBr. O estande final de plantas foi significativamente maior no tratamento no qual N não foi aplicado em cobertura, destacando-se a inoculação com AzBr, média de 96,98% de plantas estabelecidas, contra 51,56% na testemunha convencional, observando-se que até a dose média de N, houve uma maior tolerância das plantas aos sintomas de minas foliares e as viroses do Grupo Tospovirus.Azospirillum brasilense inoculation efficiency associated with rooting growth and production of lettuceAbstract: In the survey evaluated the effect of Azospirillum brasilense (AzBr inoculated with and without rooting, on growth, biomass production and lettuce pest resistance, cultivar Lucy Brown. The experiment was conducted in a greenhouse in plastic bags with 8.0 L capacity in

  1. Maize Inoculation with Azospirillum brasilense Ab-V5 Cells Enriched with Exopolysaccharides and Polyhydroxybutyrate Results in High Productivity under Low N Fertilizer Input.

    Science.gov (United States)

    Oliveira, André L M; Santos, Odair J A P; Marcelino, Paulo R F; Milani, Karina M L; Zuluaga, Mónica Y A; Zucareli, Claudemir; Gonçalves, Leandro S A

    2017-01-01

    Although Azospirillum strains used in commercial inoculant formulations presents diazotrophic activity, it has been reported that their ability to produce phytohormones plays a pivotal role in plant growth-promotion, leading to a general recommendation of its use in association with regular N-fertilizer doses. In addition, a high variability in the effectiveness of Azospirillum inoculants is still reported under field conditions, contributing to the adoption of the inoculation technology as an additional management practice rather than its use as an alternative practice to the use of chemical inputs in agriculture. To investigate whether the content of stress-resistance biopolymers would improve the viability and performance of Azospirillum inoculants when used as substitute of N-fertilizers, biomass of A. brasilense strain Ab-V5 enriched in exopolysaccharides (EPS) and polyhydroxybutirate (PHB) was produced using a new culture medium developed by factorial mixture design, and the effectiveness of resulting inoculants was evaluated under field conditions. The culture medium formulation extended the log phase of A. brasilense cultures, which presented higher cell counts and increased EPS and PHB contents than observed in the cultures grown in the OAB medium used as control. An inoculation trial with maize conducted under greenhouse conditions and using the biopolymers-enriched Ab-V5 cells demonstrated the importance of EPS and PHB to the long term bacterial viability in soil and to the effectiveness of inoculation. The effectiveness of liquid and peat inoculants prepared with Ab-V5 cells enriched with EPS and PHB was also evaluated under field conditions, using maize as target crop along different seasons, with the inoculants applied directly over seeds or at topdressing under limiting levels of N-fertilization. No additive effect on yield resulted from inoculation under high N fertilizer input, while inoculated plants grown under 80% reduction in N fertilizer

  2. Maize Inoculation with Azospirillum brasilense Ab-V5 Cells Enriched with Exopolysaccharides and Polyhydroxybutyrate Results in High Productivity under Low N Fertilizer Input

    Directory of Open Access Journals (Sweden)

    André L. M. Oliveira

    2017-09-01

    Full Text Available Although Azospirillum strains used in commercial inoculant formulations presents diazotrophic activity, it has been reported that their ability to produce phytohormones plays a pivotal role in plant growth-promotion, leading to a general recommendation of its use in association with regular N-fertilizer doses. In addition, a high variability in the effectiveness of Azospirillum inoculants is still reported under field conditions, contributing to the adoption of the inoculation technology as an additional management practice rather than its use as an alternative practice to the use of chemical inputs in agriculture. To investigate whether the content of stress-resistance biopolymers would improve the viability and performance of Azospirillum inoculants when used as substitute of N-fertilizers, biomass of A. brasilense strain Ab-V5 enriched in exopolysaccharides (EPS and polyhydroxybutirate (PHB was produced using a new culture medium developed by factorial mixture design, and the effectiveness of resulting inoculants was evaluated under field conditions. The culture medium formulation extended the log phase of A. brasilense cultures, which presented higher cell counts and increased EPS and PHB contents than observed in the cultures grown in the OAB medium used as control. An inoculation trial with maize conducted under greenhouse conditions and using the biopolymers-enriched Ab-V5 cells demonstrated the importance of EPS and PHB to the long term bacterial viability in soil and to the effectiveness of inoculation. The effectiveness of liquid and peat inoculants prepared with Ab-V5 cells enriched with EPS and PHB was also evaluated under field conditions, using maize as target crop along different seasons, with the inoculants applied directly over seeds or at topdressing under limiting levels of N-fertilization. No additive effect on yield resulted from inoculation under high N fertilizer input, while inoculated plants grown under 80% reduction in

  3. Coberturas vegetais, doses de nitrogênio e inoculação de sementes com Azospirillum brasilense em arroz de terras altas no sistema plantio direto Cover crops, nitrogen rates and seeds inoculation with Azospirillum brasilense in upland rice under no-tillage

    Directory of Open Access Journals (Sweden)

    Douglas de Castilho Gitti

    2013-01-01

    Full Text Available O arroz é importante fonte de energia e proteínas para a população mundial, principalmente na Ásia e Oceania. No Brasil, juntamente com o feijão, constitui a base da alimentação. Tecnologias sustentáveis que reduzam custos da produção e aumentem a produtividade do arroz podem garantir seu suprimento em períodos de alta demanda. O objetivo deste trabalho foi avaliar diferentes coberturas vegetais antecessoras (milheto [Pennisetum americanum], crotalária [Crotalaria juncea], guandu [Cajanus cajan], braquiária [Brachiaria ruziziensis], milheto + crotalária e milheto + guandu, doses de nitrogênio (N em cobertura (0, 40, 80 e 120 kg ha-1 e o efeito da inoculação de sementes com Azospirillum brasilense no arroz de terras altas em sistema plantio direto no desenvolvimento e na produtividade. Utilizou-se o delineamento experimental em blocos casualizados em esquema fatorial 6x4x2 com quatro repetições. O estudo foi desenvolvido em um Latossolo Vermelho, em Selvíria, Estado do Mato Grosso do Sul, Brasil, em 2011/2012. O cultivo do guandu antecedendo o arroz proporcionou maior produtividade do arroz somente em comparação a B. ruziziensi. A produtividade do arroz em função das doses de N em cobertura se ajustou a uma função quadrática. Não houve influência da inoculação de sementes com A. brasilense sobre a produtividade do arroz, porém houve interação entre a inoculação e as coberturas vegetais sobre o teor de N foliar, número de panículas por m², matéria seca de plantas de arroz e a massa de cem grãos.Rice is an important source of energy and protein for the world population, mainly in Asia and Oceania. In Brazil, together with common beans, it is the population's basic food. Sustainable technologies that reduce rice production costs and increase productivity can warrant its supply on period of high demand. The objective of this study was to evaluate different preceding cover crops (millet [Pennisetum americanum

  4. Coberturas vegetais, doses de nitrogênio e inoculação de sementes com Azospirillum brasilense em arroz de terras altas no sistema plantio direto Cover crops, nitrogen rates and seeds inoculation with Azospirillum brasilense in upland rice under no-tillage

    Directory of Open Access Journals (Sweden)

    Douglas de Castilho Gitti

    2012-01-01

    Full Text Available O arroz é importante fonte de energia e proteínas para a população mundial, principalmente na Ásia e Oceania. No Brasil, juntamente com o feijão, constitui a base da alimentação. Tecnologias sustentáveis que reduzam custos da produção e aumentem a produtividade do arroz podem garantir seu suprimento em períodos de alta demanda. O objetivo deste trabalho foi avaliar diferentes coberturas vegetais antecessoras (milheto [Pennisetum americanum], crotalária [Crotalaria juncea], guandu [Cajanus cajan], braquiária [Brachiaria ruziziensis], milheto + crotalária e milheto + guandu, doses de nitrogênio (N em cobertura (0, 40, 80 e 120 kg ha-1 e o efeito da inoculação de sementes com Azospirillum brasilense no arroz de terras altas em sistema plantio direto no desenvolvimento e na produtividade. Utilizou-se o delineamento experimental em blocos casualizados em esquema fatorial 6x4x2 com quatro repetições. O estudo foi desenvolvido em um Latossolo Vermelho, em Selvíria, Estado do Mato Grosso do Sul, Brasil, em 2011/2012. O cultivo do guandu antecedendo o arroz proporcionou maior produtividade do arroz somente em comparação a B. ruziziensi. A produtividade do arroz em função das doses de N em cobertura se ajustou a uma função quadrática. Não houve influência da inoculação de sementes com A. brasilense sobre a produtividade do arroz, porém houve interação entre a inoculação e as coberturas vegetais sobre o teor de N foliar, número de panículas por m², matéria seca de plantas de arroz e a massa de cem grãos.Rice is an important source of energy and protein for the world population, mainly in Asia and Oceania. In Brazil, together with common beans, it is the population's basic food. Sustainable technologies that reduce rice production costs and increase productivity can warrant its supply on period of high demand. The objective of this study was to evaluate different preceding cover crops (millet [Pennisetum americanum

  5. Sequencing and promoter analysis of the nifENXorf3orf5fdxAnifQ operon from Azospirillum brasilense Sp7

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    Potrich D.P.

    2001-01-01

    Full Text Available A 40-kb DNA region containing the major cluster of nif genes has been isolated from the Azospirillum brasilense Sp7 genome. In this region three nif operons have been identified: nifHDKorf1Y, nifENXorf3orf5fdxAnifQ and orf2nifUSVorf4. The operons containing nifENX and nifUSV genes are separated from the structural nifHDKorf1Y operon by about 5 kb and 10 kb, respectively. The present study shows the sequence analysis of the 6045-bp DNA region containing the nifENX genes. The deduced amino acid sequences from the open reading frames were compared to the nif gene products of other diazotrophic bacteria and indicate the presence of seven ORFs, all reading in the same direction as that of the nifHDKorf1Y operon. Consensus sigma54 and NifA-binding sites are present only in the promoter region upstream of the nifE gene. This promoter is activated by NifA protein and is approximately two-times less active than the nifH promoter, as indicated by the ß-galactosidase assays. This result suggests the differential expression of the nif genes and their respective products in Azospirillum.

  6. Enhanced performance of the microalga Chlorella sorokiniana remotely induced by the plant growth-promoting bacteria Azospirillum brasilense and Bacillus pumilus.

    Science.gov (United States)

    Amavizca, Edgar; Bashan, Yoav; Ryu, Choong-Min; Farag, Mohamed A; Bebout, Brad M; de-Bashan, Luz E

    2017-02-01

    Remote effects (occurring without physical contact) of two plant growth-promoting bacteria (PGPB) Azospirillum brasilense Cd and Bacilus pumilus ES4 on growth of the green microalga Chlorella sorokiniana UTEX 2714 were studied. The two PGPB remotely enhanced the growth of the microalga, up to six-fold, and its cell volume by about three-fold. In addition to phenotypic changes, both bacteria remotely induced increases in the amounts of total lipids, total carbohydrates, and chlorophyll a in the cells of the microalga, indicating an alteration of the microalga's physiology. The two bacteria produced large amounts of volatile compounds, including CO2, and the known plant growth-promoting volatile 2,3-butanediol and acetoin. Several other volatiles having biological functions in other organisms, as well as numerous volatile compounds with undefined biological roles, were detected. Together, these bacteria-derived volatiles can positively affect growth and metabolic parameters in green microalgae without physical attachment of the bacteria to the microalgae. This is a new paradigm on how PGPB promote growth of microalgae which may serve to improve performance of Chlorella spp. for biotechnological applications.

  7. Increased Salinity Tolerance of Cowpea Plants by Dual Inoculation of an Arbuscular Mycorrhizal Fungus Glomus clarum and a Nitrogen-fixer Azospirillum brasilense.

    Science.gov (United States)

    Rabie, G H; Aboul-Nasr, M B; Al-Humiany, A

    2005-03-01

    Pot greenhouse experiments were carried out to attempt to increase the salinity tolerance of one of the most popular legume of the world; cowpea; by using dual inoculation of an Am fungus Glomus clarum and a nitrogen-fixer Azospirillum brasilense. The effect of these beneficial microbes, as single- or dual inoculation-treatments, was assessed in sterilized loamy sand soil at five NaCl levels (0.0~7.2 ds/m) in irrigating water. The results of this study revealed that percentage of mycorrhizal infection, plant height, dry weight, nodule number, protein content, nitrogenase and phosphatase activities, as well as nutrient elements N, P, K, Ca, Mg were significantly decreased by increasing salinity level in non-mycorrhized plants in absence of NFB. Plants inoculated with NFB showed higher nodule numbers, protein content, nitrogen concentration and nitrogenase activities than those of non-inoculated at all salinity levels. Mycorrhized plants exhibited better improvement in all measurements than that of non-mycorrhized ones at all salinity levels, especially, in the presence of NFB. The concentration of Na(+) was significantly accumulated in cowpea plants by rising salinity except in shoots of mycorrhizal plants which had K(+)/Na(+) ratios higher than other treatments. This study indicated that dual inoculation with Am fungi and N-fixer Azospirillum can support both needs for N and P, excess of NaCl and will be useful in terms of soil recovery in saline area.

  8. Interação de bactérias benéficas associativas (Herbaspirillum seropedicae e Azospirillum brasilense) com diferentes espécies de gramíneas (Zea mays, Brachypodium distachyon e Setaria viridis)

    OpenAIRE

    Amaral, Fernanda Plucani do

    2014-01-01

    Tese (doutorado) - Universidade Federal de Santa Catarina, Centro de Ciências Agrárias, Programa de Pós-Graduação em Recursos Genéticos Vegetais, Florianópolis, 2014. O nitrogênio é um nutriente essencial no crescimento e desenvolvimento das plantas. A habilidade da planta em suprir a necessidade de nitrogênio necessária pode vir da fixação biológica de nitrogênio (FBN), através de interações com bactérias diazotróficas associativas. Azospirillum brasilense, que coloniza a rizosfera, e Her...

  9. Short term effects of Glomus claroideum and Azospirillum brasilense on growth and root acid phosphatase activity of Carica papaya L. under phosphorus stress.

    Science.gov (United States)

    Alarcón, Alejandro; Davies, Frederick T; Egilla, Johnatan N; Fox, Theodore C; Estrada-Luna, Arturo A; Ferrera-Cerrato, Ronald

    2002-01-01

    Arbuscular mycorrhizal fungi (AMF) are able to increase root enzymatic activity of acid and alkaline phosphatases. However, the role of AMF on phosphatase activity has not been reported in papaya (Carica papaya L.), which is frequently established at places with soil phosphorus (P) deficiencies. The goals of this research were to determine the effect of Glomus claroideum (Gc), and plant growth promoting rhizobacterium Azospirillum brasilense strain VS7 [Ab]) on root phosphatase activity and seedling growth of Carica papaya L. cv. Red Maradol under low P conditions. There were four treatments-colonization with: 1) Gc, 2) Ab, 3) Gc+Ab, and 4) non-inoculated seedlings. Plants were established in a coarse sand:sandy loam substrate under P-limitation (11 microg P ml(-1)), supplied with a modified Long Ashton Nutrient Solution. Seedling growth was severely reduced by low P. Gc+Ab inoculated plants had greater total dry matter and leaf area than non-colonized plants. Gc-inoculated plants had greater leaf area than non-colonized plants. Treatments did not differ in leaf area ratio, specific leaf area and, total chlorophyll content. There was a non-significant effect on stem relative growth rate with Gc and Gc+Ab plants. Mycorrhizal colonization enhanced the bacterial population 3.4-fold in the Gc+Ab treatment compared with the population quantified in Ab treatment. Soluble and extractable root acid phosphatase activity (RAPA) was higher in Gc inoculated plants. We discussed on the possible relation among both inoculated microorganisms and also with the P-limitation which plants were established.

  10. Metabolic profiling of two maize (Zea mays L.) inbred lines inoculated with the nitrogen fixing plant-interacting bacteria Herbaspirillum seropedicae and Azospirillum brasilense.

    Science.gov (United States)

    Brusamarello-Santos, Liziane Cristina; Gilard, Françoise; Brulé, Lenaïg; Quilleré, Isabelle; Gourion, Benjamin; Ratet, Pascal; Maltempi de Souza, Emanuel; Lea, Peter J; Hirel, Bertrand

    2017-01-01

    Maize roots can be colonized by free-living atmospheric nitrogen (N2)-fixing bacteria (diazotrophs). However, the agronomic potential of non-symbiotic N2-fixation in such an economically important species as maize, has still not been fully exploited. A preliminary approach to improve our understanding of the mechanisms controlling the establishment of such N2-fixing associations has been developed, using two maize inbred lines exhibiting different physiological characteristics. The bacterial-plant interaction has been characterized by means of a metabolomic approach. Two established model strains of Nif+ diazotrophic bacteria, Herbaspirillum seropedicae and Azospirillum brasilense and their Nif- couterparts defficient in nitrogenase activity, were used to evaluate the impact of the bacterial inoculation and of N2 fixation on the root and leaf metabolic profiles. The two N2-fixing bacteria have been used to inoculate two genetically distant maize lines (FV252 and FV2), already characterized for their contrasting physiological properties. Using a well-controlled gnotobiotic experimental system that allows inoculation of maize plants with the two diazotrophs in a N-free medium, we demonstrated that both maize lines were efficiently colonized by the two bacterial species. We also showed that in the early stages of plant development, both bacterial strains were able to reduce acetylene, suggesting that they contain functional nitrogenase activity and are able to efficiently fix atmospheric N2 (Fix+). The metabolomic approach allowed the identification of metabolites in the two maize lines that were representative of the N2 fixing plant-bacterial interaction, these included mannitol and to a lesser extend trehalose and isocitrate. Whilst other metabolites such as asparagine, although only exhibiting a small increase in maize roots following bacterial infection, were specific for the two Fix+ bacterial strains, in comparison to their Fix- counterparts. Moreover, a number

  11. Metabolic profiling of two maize (Zea mays L. inbred lines inoculated with the nitrogen fixing plant-interacting bacteria Herbaspirillum seropedicae and Azospirillum brasilense.

    Directory of Open Access Journals (Sweden)

    Liziane Cristina Brusamarello-Santos

    Full Text Available Maize roots can be colonized by free-living atmospheric nitrogen (N2-fixing bacteria (diazotrophs. However, the agronomic potential of non-symbiotic N2-fixation in such an economically important species as maize, has still not been fully exploited. A preliminary approach to improve our understanding of the mechanisms controlling the establishment of such N2-fixing associations has been developed, using two maize inbred lines exhibiting different physiological characteristics. The bacterial-plant interaction has been characterized by means of a metabolomic approach. Two established model strains of Nif+ diazotrophic bacteria, Herbaspirillum seropedicae and Azospirillum brasilense and their Nif- couterparts defficient in nitrogenase activity, were used to evaluate the impact of the bacterial inoculation and of N2 fixation on the root and leaf metabolic profiles. The two N2-fixing bacteria have been used to inoculate two genetically distant maize lines (FV252 and FV2, already characterized for their contrasting physiological properties. Using a well-controlled gnotobiotic experimental system that allows inoculation of maize plants with the two diazotrophs in a N-free medium, we demonstrated that both maize lines were efficiently colonized by the two bacterial species. We also showed that in the early stages of plant development, both bacterial strains were able to reduce acetylene, suggesting that they contain functional nitrogenase activity and are able to efficiently fix atmospheric N2 (Fix+. The metabolomic approach allowed the identification of metabolites in the two maize lines that were representative of the N2 fixing plant-bacterial interaction, these included mannitol and to a lesser extend trehalose and isocitrate. Whilst other metabolites such as asparagine, although only exhibiting a small increase in maize roots following bacterial infection, were specific for the two Fix+ bacterial strains, in comparison to their Fix- counterparts

  12. Elemental composition of strawberry plants inoculated with the plant growth-promoting bacterium Azospirillum brasilense REC3, assessed with scanning electron microscopy and energy dispersive X-ray analysis.

    Science.gov (United States)

    Guerrero-Molina, M F; Lovaisa, N C; Salazar, S M; Díaz-Ricci, J C; Pedraza, R O

    2014-07-01

    The elemental composition of strawberry plants (Fragaria ananassa cv. Macarena) inoculated with the plant growth-promoting bacterium Azospirillum brasilense REC3, and non-inoculated controls, was studied using scanning electron microscopy (SEM) and energy dispersive X-ray (EDS) analysis. This allowed simultaneous semi-quantification of different elements in a small, solid sample. Plants were inoculated and grown hydroponically in 50% or 100% Hoagland solution, corresponding to limited or optimum nutrient medium, respectively. Bacteria-inoculated plants increased the growth index 45% and 80% compared to controls when grown in 100% and 50% Hoagland solution, respectively. Thus, inoculation with A. brasilense REC3 in a nutrient-limited medium had the strongest effect in terms of increasing both shoot and root biomass and growth index, as already described for Azospirillum inoculated into nutrient-poor soils. SEM-EDS spectra and maps showed the elemental composition and relative distribution of nutrients in strawberry tissues. Leaves contained C, O, N, Na, P, K, Ca and Cu, while roots also had Si and Cl. The organic fraction (C, O and N) accounted for over 96.3% of the total chemical composition; of the mineral fraction, Na had higher accumulation in both leaves and roots. Azospirillum-inoculated and control plants had similar elemental quantities; however, in bacteria-inoculated roots, P was significantly increased (34.33%), which constitutes a major benefit for plant nutrition, while Cu content decreased (35.16%). © 2013 German Botanical Society and The Royal Botanical Society of the Netherlands.

  13. Efecto de la coinmovilización de la microalga Chlorella sorokiniana con la bacteria promotora de crecimiento vegetal Azospirillum brasilense, en el crecimiento poblacional microalgal y capacidad de remoción de amonio como uso potencial en el tratamiento..

    Directory of Open Access Journals (Sweden)

    Guillot Monroy Gabriel

    2006-06-01

    Full Text Available Se desarrollaron dos experimentos de coinmovilización de la microalga Chlorella sorokiniana con la bacteria promotora
    de crecimiento vegetal (PGPB Azospirillum brasilense en esferas de alginato, para evaluar la influencia de la bacteria en el crecimiento poblacional y en la capacidad de remoción de amonio de la microalga; con el fin de establecer el potencial que pueda tener la coinmovilización de estos microorganismos en el tratamiento terciario de aguas residuales. Los resultados revelan un significativo incremento de la población cuando C.
    sorokiniana es coinmovilizada con A. brasilense, aunque no se observó un efecto significativo en la remoción de amonio. Los resultados obtenidos en el presente trabajo, reflejan la importancia de avanzar en el estudio de un sistema coinmovilizado altamente eficiente para ser utilizado en tratamiento de aguas residuales. La coinmovilización
    de C. sorokiniana con A. brasilense, muestra la habilidad de esta bacteria para influir de manera positiva en el crecimiento y fisiología de la microalga y por lo tanto la remoción de amonio puede ser incrementada.

  14. Dänemark: Der Gute Krieg

    DEFF Research Database (Denmark)

    Jakobsen, Peter Viggo

    2015-01-01

    on Danish foreign and security policy is that Denmark had a good war. Denmark did not go to war in Afghanistan to win. Denmark went to war to support the right causes (anti-terrorism, human rights and democracy) and the right allies (United States, United Kingdom and NATO) in order to generate pride at home...... discernible impact on Danish foreign and security policy. Denmark has continued to engage in new combat operations led by the major NATO powers. Danish F-16s dropped 988 smart munitions during war in Libya in 2011 (11% of the NATO total), it was one of the few nations to support the US call for air strikes...

  15. Quantenmechanik im Kalten Krieg David Bohm und Richard Feynman

    CERN Document Server

    Forstner, Christian

    2007-01-01

    Mitte des 20. Jahrhunderts entwickelten David Bohm und Richard Feynman zwei grundlegend verschiedene Ansätze der moderne Quantenmechanik: Bohm eine realistische Deutung mit Hilfe verborgener Parameter und Feynman den Pfadintegralformalismus. Dies ist umso bemerkenswerter, weil beide Physiker von ähnlichen Voraussetzungen ausgingen und aus ähnlichen Zusammenhängen stammten. Durch ihren vergleichenden Ansatz bietet diese Studie mehr als einen Beitrag zur Geschichte der Quantentheorie. Mit der Frage nach den sozialen und kulturellen Bedingungen der Theoriebildung ist sie darüberhinaus von wissenschaftssoziologischem und wissenschaftstheoretischem Interesse. Die anfangs ähnliche und später unterschiedliche Einbindung der beiden Wissenschaftler in die Scientific Community erlaubt es überdies zu untersuchen, welchen Anpassungsdruck die jeweilige Gruppe auf den individuellen Wissenschaftler und die Kernbestandteile seiner Forschungen ausübt und welche neuen Freiheitsgrade für die Theoriebildung entstehen, ...

  16. Die Tötung des Unschuldigen, insbesondere im Krieg

    Directory of Open Access Journals (Sweden)

    Harald Maihold

    2007-03-01

    Full Text Available Darf man einen Unschuldigen töten? Mit dieser heute noch aktuellen Frage hatten sich bereits die Theologen und Juristen im Spanien des 16. Jahrhunderts auseinanderzusetzen: Darf der Richter im Prozess aufgrund falscher Zeugen wider besseres Wissen einen Unschuldigen zum Tode verurteilen? Ist eine christliche Stadt zur Tötung oder Auslieferung eines Predigers an einen heidnischen Tyrannen berechtigt, wenn davon ihre Existenz abhängt? Muss sich der Prediger von sich aus für das Gemeinwesen opfern? Der Beitrag zeigt, wie neben das „strafrechtliche“ Schuldmodell, wonach der Unschuldige keinen Nachteil verdient und nicht getötet werden darf, in der Spanischen Spätscholastik ein stärker auf Nutzenargumente, insbesondere das Gemeinwohl, abstellendes Utilitätsmodell tritt, das die Tötung des Unschuldigen aus schuldfremden Gründen erlaubt. Damit geht ein Wandel des Staatsverständnisses einher – weg vom Gottesstaat hin zum irdischen Machtstaat, der das Leben seiner Bürger für das Gemeinwesen opfert.

  17. Beyond Vom Kriege: The Character and Conduct of Modern War

    National Research Council Canada - National Science Library

    Hooker, Jr, R. D

    2005-01-01

    It is the tragedy of history that man cannot free himself from war. Indeed, far more than by the development of art or literature or trade or political institutions, the history of man has been determined by the wars he has fought...

  18. Polymers selection for a liquid inoculant of Azospirillum brasilense ...

    African Journals Online (AJOL)

    Sandra Lucia Cortes Patiño

    2015-08-19

    Aug 19, 2015 ... Sci. Asia 33(1):69-77. Velineni S, Brahmaprakash GP (2011). Survival and phosphate solubilizing ability of Bacillus megaterium in liquid inoculants under high temperature and desiccation stress. J. Agric. Sci. Technol. 13(5):795-802. Vessey JK (2003) Plant growth promoting rhizobacteria as biofertilizers.

  19. Der Hundertjährige Krieg (1558-1660/61) in Estland / Margus Laidre

    Index Scriptorium Estoniae

    Laidre, Margus, 1959-

    2006-01-01

    Ülemvõimu pärast Läänemerel peetud sõdade perioodist, mida autor nimetaks Eesti ajaloo Saja-aastaseks sõjaks (1558-1660/61), mis jaguneks : 1) Vene-Liivi etapp 1558-1562, 2) Taani-Poola etapp 1563-1570, 3) Vene etapp 1570-1595, 4) 1595-1599 vaheetapp, 5) 1600-1629 "kolmekümneaastane sõda Rootsi ja Poola vahel, 6) 1629-1654 vaherahude etapp, 1655-1660/61 Rootsi triumf. Eesti rahvastiku suurusest 16.-18. sajandil

  20. Krieg der Bilder - Zur Zugänglichmachung österreichischer Filmquellen zum Ersten Weltkrieg

    Directory of Open Access Journals (Sweden)

    Thomas Ballhausen

    2014-06-01

    Full Text Available Schon in der Frühzeit des Films machten sich die Verantwortlichen und Produzenten Gedanken, wie Material dauerhaft bewahrt, sinnvoll archiviert und wieder zugänglich gemacht werden könnte: Der Wunsch nach der adäquaten Sicherung, Lagerung und weiteren Bearbeitung unter Achtung der dualen Verantwortlichkeit gegenüber den Beständen als auch der Öffentlichkeit – der Kernaufgaben eines jeden Archivs – ist bereits für das späte 19. Jahrhundert dokumentiert. Für eine zeitgemäße Erleichterung im seriösen Materialzugriff bieten wissenschaftliche Editionen und das Internet hervorragende Voraussetzungen. Insbesondere am Beispiel der österreichischen Filmquellen zum Ersten Weltkrieg lassen sich die Vorzüge beider Vermittlungsoptionen verdeutlichen.

  1. 10 Jahre danach: Der Irak-Krieg 2003 und das (Medien-Schlachtfeld III

    Directory of Open Access Journals (Sweden)

    Karl H. Stingeder

    2013-09-01

    Full Text Available Im dritten Teil seiner dreiteiligen Analyse des Irakkriegs und der >embedded journalists< untersucht Karl H. Stingeder die Informationsstrategie der US-Regierung und prüft die Frage, ob die mediale Inszenierung der US-Regierung als politische Propaganda gewertet werden kann.

  2. "Croatian Memories": eine Interviewsammlung mit personlichen Berichten uber Krieg und Traumata

    NARCIS (Netherlands)

    van Hessen, Adrianus J.; de Jong, Franciska M.G.; Scagliola, Stef; Apostolopoulos, Nicolas; Pagenstecher, Cord

    2013-01-01

    This book chapter summarizes the aims and methodology underlying the collection oral history interviews that has been generated in the context of the project Croatian Memories. It includes an overview of facts and figures for the contents of the collection and the interviewees.

  3. Rezension zu: Insa Eschebach, Regina Mühlhäuser (Hg.: Krieg und Geschlecht. Sexuelle Gewalt im Krieg und Sex-Zwangsarbeit in NS-Konzentrationslagern. Berlin: Metropol Verlag 2008.

    Directory of Open Access Journals (Sweden)

    Veronika Springmann

    2009-10-01

    Full Text Available In recent years, the conflict with protagonists of and functions of violence has accelerated, as has research on and interpretation of sexual violence. The conference volume collects new contributions to research on and interpretive patterns of sexual violence in war and in sexual forced labor in National Socialist concentration camps, in order first to depict the current state of research and second to look for future perspectives. The brilliant essays inform readers on the current questions and debates on these topics.

  4. Rezension: Habsburgs schmutziger Krieg – Ermittlungen zur österreichisch-ungarischen Kriegsführung 1914 – 1918

    Directory of Open Access Journals (Sweden)

    Paul Winkler

    2015-06-01

    Full Text Available Ein AutorInnenkollektiv hat sich mit dieser Publikation dem schmutzigen 1. Weltkrieg der Habsburger zwischen 1914 und 1918 gewidmet und präsentiert dabei bedeutende Ergebnisse. Paul Winkler hat für die MEDIENIMPULSE rezensiert ... Beschlüssen, Kalkulationen und Kontrollverlust spüren Hannes Leidinger, Verena Moritz, Karin Moser und Wolfram Dornik in ihrer problemorientierten Untersuchung der dunkelsten Kapitel des letzten Waffengangs der Habsburger nach. Eine erschreckende Vielzahl dokumentierter Pflichtverletzungen und Gräuel erhellt Mechanismen der Gewalteskalation gegen pauschal verdächtigte Massen. Eine quellenorientierte Pionierleistung mit zweifelsohne nachhaltiger Beeinflussung einer österreichischen Erinnerungskultur – auch über das Gedenkjahr 2014 hinaus.

  5. Die Eroberung der Atmosphäre: Wetterbeeinflussung in Süddeutschland zur Zeit des Kalten Krieges

    DEFF Research Database (Denmark)

    Achermann, Dania

    2013-01-01

    on the activities at the Institut flir Physik der Atmosphäre in Southern Bavaria, where fog dissipation and hail control were prirnarily developed for civilian and local purposes. This example depicts the relationship between the research conducted there and American developments, and demonstrates how the local...

  6. Synthesis of poly(3-hydroxybutyrate using enzymes Azospirillum brasilense in vitro

    Directory of Open Access Journals (Sweden)

    Soheila Abbasi

    2017-06-01

    Full Text Available Introduction:Polyhydroxyalkaniates (PHAS are bacterial polymers that are formed as naturally occurring storage polyesters by a wide range of microorganisms usually under unbalanced growth conditions. Mechanical properties of PHAS make them suitable replacements for petrochemically produced bulk plastics (polyethylene, polypropylene etc., but in contrast to these commodity plastics PHA are completely degradable to carbon dioxide and water through natural microbiological mineralization. PHAS can be produced by biotechnological processes under controlled conditions. Polyhydroxybotyric acid (PHB was the first of thePHAS discovered and is the most abundant polyester found in bacteria. Materials and methods: First, a rich bacterial suspension prepared from poly(3-hydroxybutyrate, then the cells were broken down and ensure the absence of live cells.The cell free enzymes were added to M9 medium.Then the sample turbidity was measured at a wavelength of 600 nm and incubated at 30°C and centrifiuged at 120rpm. Next, the amount of PHB as well as solution turbidity were measured and compared with control. The physical and chemical analyzes of extracted PHB samples such as UV absorbtion, FTIR, HPLC and HNMR assesed and compared with standard sample. Results: In twelve days this experiment was conducted, from the third day, the amount of polyhydroxybutyrate was measurable and the highest polyhydroxybutyrate was gained on the tenth day. The level of PHB was constant. Moreover, the level of glucose was decreased gradually till the tenth day. By Physico-chemical analysis, production of polyhydroxybutyrate was approved. Discussion and conclusion: In this experiment, the reaction efficiency was 37.5 percent. Probably the rest of glucose could be changed to other intermediate compounds by other enzymes in the sample.Therefore, by purification of the enzyme, usage of specific substrate and optimization of conditions outside the cell could help the production of polyhydroxybutyrate continually. Production is very cost-effective, by this method.

  7. Preußische Verwaltung in der Provinz:Entstehung, Personal, Aufgaben und Leistungen der Kriegs- und Domänenkammer in Hamm 1767 bis 1806

    OpenAIRE

    Ditté, R. (Rainer)

    2011-01-01

    Gegenstand der folgenden Studie ist die Verwaltungstätigkeit der 1767 in Hamm für die Grafschaft Mark etablierten preußischen Provinzialverwaltungsbehörde während der knapp 40 Jahre ihres Bestehens. Ziel ist, ihre Aufgaben zu verdeutlichen und zu untersuchen, in welchem Maße ihr Handeln durch die Einbindung in die zentrale Verwaltungsorganisation bestimmt und durch die politischen und gesellschaftlichen Rahmenbedingungen einer Epoche zögernd einsetzender Reformen beeinflusst wurde. Damit soll...

  8. Die Pugwash Conferences on Science and World Affairs Ein Beispiel für erfolgreiche „Track-II-Diplomacy“ der Naturwissenschaftler im Kalten Krieg

    Science.gov (United States)

    Neuneck, Götz

    "Kein Zeitalter der Geschichte ist stärker von den Naturwissenschaften durchdrungen und abhängiger von ihnen als das 20. Jahrhundert" schreibt Eric Hobsbawn im Kapitel "Zauberer und Lehrlinge: Die Naturwissenschaften" seines Buches "Zeitalter der Extreme".

  9. Cover crops, nitrogen rates and seeds inoculation with Azospirillum brasilense in upland rice under no-tillage

    OpenAIRE

    Douglas de Castilho Gitti; Orivaldo Arf; José Roberto Portugal; Daiene Camila Dias Chaves Corsini; Ricardo Antônio Ferreira Rodrigues; Flávio Hiroshi Kaneko

    2012-01-01

    O arroz é importante fonte de energia e proteínas para a população mundial, principalmente na Ásia e Oceania. No Brasil, juntamente com o feijão, constitui a base da alimentação. Tecnologias sustentáveis que reduzam custos da produção e aumentem a produtividade do arroz podem garantir seu suprimento em períodos de alta demanda. O objetivo deste trabalho foi avaliar diferentes coberturas vegetais antecessoras (milheto [Pennisetum americanum], crotalária [Crotalaria juncea], guandu [Cajanus caj...

  10. Fourth-Generation War and Other Myths

    Science.gov (United States)

    2005-11-01

    while the second trinity accords with the subjective nature of war. Carl von Clausewitz , Vom Kriege. Hinterlassenes Werk des Generals Carl von Clausewitz , 19th...kleinem Krieg, gehalten auf der Kriegs-Schule 1810 und 1811," in Werner Hahlweg, ed., Carl von Clausewitz : Schriften -Aufsdtze - Studien - Briefe

  11. Absorption efficiency of N, P, K through triple inoculation of wheat (Triticum aestivum L.) by Azospirillum brasilense, Streptomyces sp., Glomus intraradices and manure application.

    Science.gov (United States)

    Ardakani, Mohammad Reza; Mazaheri, Daryush; Mafakheri, Saeed; Moghaddam, Ali

    2011-04-01

    This study examined the effects of different biofertilizers and manure on the absorption and absorption efficiency of macronutrients by wheat in Alborz Province, Iran. The experimental design was factorial in the form of a randomized complete block design with three replications. The four factors of the experiment were Azospirillum (with 600 g/ha and without), mycorrhiza (with 1 kg/ha and without), Streptomyces (with 0.5 kg/ha and without) and manure (with 30 t/ha and without). To measure N, P and K, plants were harvested one time when grains were at dough stage and another time at plant maturity, oven dried, grinded and analyzed. Azospirillum, mycorrhiza and manure had a significant effect (p ≤ 0.01) on most of the measured traits, but the effect of Streptomyces was not significant. The two-fold and three-fold interactions significantly affected the absorption and absorption efficiency of the three macronutrients. In most cases, four-fold interactions yielded the highest values. Overall, the experimental results indicate that biofertilizers and manure can naturally meet most of the plant's nutritional requirements, improve the efficiency of nutrient absorption and replace part of chemical fertilizers in sustainable agricultural production systems.

  12. Absorption efficiency of N, P, K through triple inoculation of wheat (Triticum aestivum L.) by Azospirillum brasilense, Streptomyces sp., Glomus intraradices and manure application

    OpenAIRE

    Ardakani, Mohammad Reza; Mazaheri, Daryush; Mafakheri, Saeed; Moghaddam, Ali

    2011-01-01

    This study examined the effects of different biofertilizers and manure on the absorption and absorption efficiency of macronutrients by wheat in Alborz Province, Iran. The experimental design was factorial in the form of a randomized complete block design with three replications. The four factors of the experiment were Azospirillum (with 600 g/ha and without), mycorrhiza (with 1 kg/ha and without), Streptomyces (with 0.5 kg/ha and without) and manure (with 30 t/ha and without). To measure N, ...

  13. Estandarización de un medio de cultivo a partir de fuentes agroindustriales para la multiplicación de Azospirillum brasilense

    Directory of Open Access Journals (Sweden)

    Diego Rivera

    2012-07-01

    Full Text Available The Plackett-Burman statistical design was initially applied for Screening the most important nutritional variables (four carbon sources, two nitrogen sources, K2 HPO4 y MgSO4 and a trace elements solution affecting microorganism biomass production. Subsequently, a optimising the process by applied a BoxBehnken design for the most important factors tree. The results showed that biomass (UFC/mL production was strongly affected by nutritional and physico conditions of culture medium. The highest yield was obtained in the following conditions: 3g/L sucrose, 6,75 mL/L molasses, 0,77 mL/L glycerol, 14,42 mL/L soybean hydrolyzate, 0,848 g/L yeast hidrolyzate, 0,25 g/L K2 HPO4 , ⅗ aeration and shaking at 120 rpm 26 hours total fermentation time during which 1 x 109 UFC/mL were obtained.

  14. "Death Does Not Rot": Transitional Justice and Local “Truths” in the Aftermath of the War in Northern Uganda "Death does not rot": Transitional Justice und lokale “Wahrheiten” im vom Krieg gezeichneten Norduganda

    Directory of Open Access Journals (Sweden)

    Barbara Meier

    2013-01-01

    Full Text Available The article looks at the way Acholi in northern Uganda address war-related matters of “peace” and “justice” beyond the mainstream human rights discourse reflecting some of the basic concepts that are decisive for the way people deal with transitional and local justice. The relationality and the segmentary structure of Acholi society play major roles in categorising “peace” and “war” while being at odds with the globalised standards of human rights that have been brought into play by international agencies, civil society and church organisations as well as the Ugandan state. A major argument is that a one-dimensional understanding of the cosmological underpinnings of rituals as a locally embedded tool of transitional justice (TJ has an impact on the failure of TJ in northern Uganda. Thus the article highlights the specific cultural dilemmas in which the process of peace currently appears to be stuck.Dieser Beitrag untersucht, wie Acholi in Norduganda jenseits des Menschenrechtsdiskurses „Frieden“ und „Gerechtigkeit“ verstehen, und zeigt grundlegende lokale Denkweisen und Rechtsvorstellungen auf, die für ihren Umgang mit Transitional-Justice-Verfahren entscheidend sind. Bei der Definition der Begriffe „Krieg“ und „Frieden“ spielen Relationalität und Segmentarität der Acholi-Gesellschaft eine wesentliche Rolle, stehen aber im Gegensatz zu den globalisierten Menschenrechtsstandards, wie sie von internationalen Akteuren, zivilgesellschaftlichen Gruppen, kirchlichen Organisationen und auch dem ugandischen Staat vertreten werden. Die Autorin betont, dass ein eindimensionales Verständnis der kosmologischen Hintergründe von Ritualen, die für Transitional-Justice-Maßnahmen herangezogen wurden, zum Scheitern des Konzepts der Transitional Justice in Norduganda beigetragen hat. Sie identifiziert die spezifischen kulturellen Dilemmata, in denen der Friedensprozess in Norduganda derzeit festzustecken scheint.

  15. Experts in the cold war. War experiences and peace conceptions of US-American physicists 1920-1963; Experten im Kalten Krieg. Kriegserfahrungen und Friedenskonzeptionen US-amerikanischer Kernphysiker 1920-1963

    Energy Technology Data Exchange (ETDEWEB)

    Wunderle, Ulrike

    2015-07-01

    The study is dedicated to the American elite of nuclear physicists, which explained after the second world war the possibilities of their science for war and peace. What induced their thinking and handling? The focus lies on the scientific shapings and war experiences of the first really international generation of physicists, which began in the 1920th years their career before many of their representatives had to fly from the NS regime from Europe and cooperated in the Manhattan project in the construction of the atomic bomb. These experiences the author refers to in order to get on the track of the explanations of the exper elite in the immediate afterwar time and in the cold war. How far their internationally and by actual war experiences shaped thinking about their own contribution as scientist to the national security - in the sense of discouragement or the cooperative conflict regulation - found its expression in the cold war, is drawn on different action levels of the participants from the Geneve conference ''Atoms for Peace'' of 1955 until the signing of the so-called Limited Test Ban Treaty of 1963.

  16. The public organization of feelings and the nuclear threat. On the political psychology of war and peace. Oeffentliche Gefuehlsorganisation und nukleare Bedrohung. Zur politischen Psychologie von Krieg und Frieden

    Energy Technology Data Exchange (ETDEWEB)

    Koenig, H.

    1987-07-01

    Lasting control of the new world-threatening potential for destruction is only possible if the passionate, instinctive nature of man is reflected on the social and individual level. The instincts and instinctive desires which are suppressed in every human social process emerge in (mostly subconscious) fantasies of total power, which the state makes use of: it does not prevent the injustice fed by instinctive desires but monopolizes it. (orig.).

  17. En torno a la esencia del poder. Un estudio comparativo entre Max Scheler y José Ortega y Gasset

    National Research Council Canada - National Science Library

    Ricardo Gibu Shimabukuro

    2015-01-01

    Ortega’s admiration for Scheler ’s renowned and distinctive way of thinking can be contrasted with his harsh criticism of the relationship between life and war presented in Der Genius des Krieges und der deutsche Krieg (1915). «Life...

  18. Veröffentlichungen des Landesarchivs Schleswig-Holstein / Landesarchiv Schleswig-Holstein publikationer

    OpenAIRE

    Spoorendonk, Anke; Hering, Rainer; De Wulf, Peter; Rosenplänter, Johannes; Rathjen, Jörg; Adriansen, Inge; Hansen Nielsen, Leif; Schultz Hansen, Hans; Imberger, Elke; Jørgensen, Steffen Elmer; Frandsen, Steen Bo; Krieger, Martin; Göttsch-Elten, Silke; Schmidt-Elsaeßer, Eberhard

    2016-01-01

    Der deutsch-dänische Krieg von 1864 ist in der Literatur eingehend untersucht worden – soweit es sich um die großpolitischen und militärischen Ereignisse handelt. Das Interesse für die Verhältnisse der Zivilbevölkerung, die Zivilverwaltung und die Nationalbewegungen während des Krieges sowie auch für die wirtschaftlichen Folgen und die Identitätenbildung nach dem Krieg ist dagegen bisher gering gewesen. Um dies zu ändern, haben deutsche und dänische Historikerinnen und Historiker dieses Buch ...

  19. Disease: H00119 [KEGG MEDICUS

    Lifescience Database Archive (English)

    Full Text Available rt N, Raemaekers T, Quelhas D, Jaeken J, Mills P, Winchester B, Krieger M, Annaert W, Matthijs G ... TITLE ... ..., Zeevaert R, Mills P, Garcia-Silva MT, Briones P, Winchester B, Morelle W, Krieg

  20. Características agronômicas do trigo em função de Azospirillum brasilense, ácidos húmicos e nitrogênio em casa de vegetação

    National Research Council Canada - National Science Library

    Rodrigues, Luan F. O. S; Guimarães, Vandeir F; Silva, Mônica B. da; Pinto Junior, Artur S; Klein, Jeferson; Costa, Andreia C. P. R. da

    2014-01-01

    ... húmicos e diferentes doses de nitrogênio nas características agronômicas do trigo. Os experimentos foram instalados no dia 21 de maio de 2011 e a colheita foi procedida no dia 13 de setembro de 2011...

  1. 想起と記憶 : ギュンター・グラス『玉ねぎの皮をむきながら』をめぐって

    OpenAIRE

    依岡, 隆児

    2007-01-01

    Hier wird das Problem der Erinnerung und des Gedachtnisses in Gunter Grass' autobiographischem Werk Beim Hauten der Zwiebel (2006) erortert. Das Werk ruft wegen des Grass 'schen Bekenntnisses Mitglied der Waffen SS gewesen zu sein, groBe Sensation hervor, aber stellt zugleich das Problem in Bezug auf das Gedenken an den Krieg und das Erinnern daran in Deutschland und in den anderen Landern dar. Seit dem Krieg sind schon 60 Jahre vergangen, und die Tendenz herrscht jetzt, dass die Nachkriegsze...

  2. [Effect of Azospirillum lectins on the Activity of Proteolytic Enzymes and Their Inhibitors in Wheat Seedling Roots].

    Science.gov (United States)

    Alen'kina, S A; Nikitina, V E

    2015-01-01

    The lectins of associative nitrogen-fixing strains Azospirillum brasilense Sp7 and Sp245 were shown to exerte a multidirectional effect on the activity of acidic (pH 3.5), neutral (6.8), and alkaline (pH 7.8) proteinases. The lectin of the epiphytic A. brasilense Sp7 decreased proteolytic activity at all pH values, whereas the lectin of the endophytic A. brasilense Sp245 activated neutral and alkaline proteinases, while not affecting the alkaline ones. Experiments with protease inhibitors made it possible to conclude that the lectins of the studied A. brasilense strains alter the ratio between the activities of different protease types in germinating seeds. The activity of trypsin inhibitors in wheat seedling roots was found to increase in the presence of the lectins. Our results indicate a broader spectrum of effects of azospirilla lectins on the host plant organism.

  3. Partial characterization of nif genes from the bacterium Azospirillum amazonense

    Directory of Open Access Journals (Sweden)

    D.P. Potrich

    2001-09-01

    Full Text Available Azospirillum amazonense revealed genomic organization patterns of the nitrogen fixation genes similar to those of the distantly related species A. brasilense. Our work suggests that A. brasilense nifHDK, nifENX, fixABC operons and nifA and glnB genes may be structurally homologous to the counterpart genes of A. amazonense. This is the first analysis revealing homology between A. brasilense nif genes and the A. amazonense genome. Sequence analysis of PCR amplification products revealed similarities between the amino acid sequences of the highly conserved nifD and glnB genes of A. amazonense and related genes of A. brasilense and other bacteria. However, the A. amazonense non-coding regions (the upstream activator sequence region and the region between the nifH and nifD genes differed from related regions of A. brasilense even in nitrogenase structural genes which are highly conserved among diazotrophic bacteria. The feasibility of the 16S ribosomal RNA gene-based PCR system for specific detection of A. amazonense was shown. Our results indicate that the PCR primers for 16S rDNA defined in this article are highly specific to A. amazonense and can distinguish this species from A. brasilense.

  4. Anmeldelser af litteratur om den græsk-romerske oldtid

    DEFF Research Database (Denmark)

    Krasilnikoff, Jens

    2007-01-01

    Anmeldelse af Neville Morley, "Trade in Classical Antiquity". Key Themes in Ancient History. Cambridge UP, 2007. p.22. Anmeldelse af J. Bingen, "Hellenistic Egypt. Monarchy, Society, economy, Culture", Edinburgh UP, 2007, pp.22-23. Anmeldelse af C. Koehn, "Krieg-Diplomatie-Ideologie. Zur Ausenpol......Anmeldelse af Neville Morley, "Trade in Classical Antiquity". Key Themes in Ancient History. Cambridge UP, 2007. p.22. Anmeldelse af J. Bingen, "Hellenistic Egypt. Monarchy, Society, economy, Culture", Edinburgh UP, 2007, pp.22-23. Anmeldelse af C. Koehn, "Krieg-Diplomatie-Ideologie. Zur...

  5. Supplement to: Astronomical ephemerides, navigation and war. The astonishing cooperation of the ephemeris institutes of Germany, England, France and the USA during the Second World War based on documents in the archives of the Astronomisches Rechen-Institut. Scans of the documents. (German Title: Supplement zu: Astronomische Ephemeriden, Navigation und Krieg. Die erstaunliche Zusammenarbeit der Ephemeriden-Institute von Deutschland, England, Frankreich und den USA im Zweiten Weltkrieg nach Dokumenten im Archiv des Astronomischen Rechen-Instituts. Scans der Dokumente.)

    Science.gov (United States)

    Wielen, Roland; Wielen, Ute

    In a previous paper (Wielen R. und Wielen U. 2016a: Astronomical Ephemerides, Navigation and War), we have presented the astonishing cooperation of the ephemeris institutes of Germany, England, France and the USA during the Second World War. We were able to use numerous archivalia which we also describe and comment in that paper. In the present paper, we publish colour scans of these archivalia. All the documents shown here are held in the archives of the Astronomisches Rechen-Institut in Heidelberg.

  6. Astronomical ephemerides, navigation and war. The astonishing cooperation of the ephemeris institutes of Germany, England, France and the USA during the Second World War based on documents in the archives of the Astronomisches Rechen-Institut. Edition of the documents. (German Title: Astronomische Ephemeriden, Navigation und Krieg. Die erstaunliche Zusammenarbeit der Ephemeriden-Institute von Deutschland, England, Frankreich und den USA im Zweiten Weltkrieg nach Dokumenten im Archiv des Astronomischen Rechen-Instituts. Edition der Dokumente.)

    Science.gov (United States)

    Wielen, Roland; Wielen, Ute

    During the whole period of the Second World War, England and the USA have exchanged astronomical ephemerides with Germany, even though these data were used for the navigation of warships and aircraft and were therefore of war importance. This astonishing fact is attested by numerous documents which survived in the archives of the Astronomisches Rechen-Institut (ARI). In Germany, the exchange was even explicitly authorized by the ministry which supervised the ARI (i.e. the Reichserziehungsminister). We present here examples of ephemerides for the Sun, the Moon, planets and stars, and explain the position determination by means of astronomical data. Ephemerides were published in almanacs which were computed and issued by special ephemeris institutes. We describe the agreements on the international exchange of ephemerides which were reached in peace times, and the continuation of this exchange during the war using intermediaries in neutral countries, first in the USA (U.S. Naval Observatory, USNO), and, from 1942 onwards, in Sweden (Stockholm Observatory). Involved persons were especially H. Spencer Jones (Astronomer Royal, Greenwich), J. F. Hellweg und W. J. Eckert (USNO), B. Lindblad (Sweden), and A. Kopff (ARI). All those relevant documents which are hold in the archives of the ARI, are described and annotated in detail. Scans of these documents are presented in a separate supplement.

  7. Vom Dämonischen. Zur politischen Medientheorie des Daniel Suarez

    Directory of Open Access Journals (Sweden)

    Alessandro Barberi

    2012-12-01

    Full Text Available Die Romane des Programmierers Daniel Suarez sind weit mehr als reine Fiktionen. Seine terminatorischen Inszenierungen des Endkampfs zwischen Maschinen und Menschen basieren auf äußerst realen Studien zu derzeitigen (Kriegs-Technologien, die als politische Steuerungsmedien eingesetzt werden.This is a review of Daniel Suarez's books Daemon (2009, Darknet (2011 and Kill Decision (2013.

  8. 78 FR 51275 - Members of Senior Executive Service Performance Review Boards

    Science.gov (United States)

    2013-08-20

    ..., Executive Director, Office of Equity, Diversity and Inclusion, Office of the Commissioner Paul D. DeNard... Government Entities (TEGE) ] Gregory E. Kane, Deputy Chief Financial Officer, Chief Financial Office (CFO) David A. Krieg, IRS Human Capital Officer, Human Capital Office (HCO) Pamela J. LaRue, Chief Financial...

  9. Vaimuliku ja ajalookirjutaja Timann Brakeli "Kristlik vestlus" (1579) kui alahinnatud ajalooallikas / Aija Sakova-Merivee

    Index Scriptorium Estoniae

    Sakova-Merivee, Aija, 1980-

    2014-01-01

    Arvustus: Timann Brakel, Peter von Brakel. Christlich Gesprech. Darstellung der Geshcichte Livlands vor und während des "Livländischen Krieges" bis 1578 in gereimter Dialogform und Prosa von dem Prediger und Geschichtsschreiber Timann Brakel. Originaltext und Kommentaren, Erzählungen, Bildern und dem Lebenslauf des Timann Brakel. (Beiträge zur baltischen Geschichte, Band 19). Hirschheydt, 2012

  10. 2235-IJBCS-Article-Rachelle Ida Yapo

    African Journals Online (AJOL)

    hp

    volcano-sédimentaires de côte d'ivoire. International Journal of Innovation and. Applied Studies, 2: 372-383. Nikiema J, Gläber W, Krieg R, Schirmer M. 2013. ... groundwater vulnerability based on a modified drastic model, GIS and an analytic hierarchy process (AHP) method: the case of Egirdir Lake basin. (Isparta,.

  11. Antibacterial activity of whole plant extract of Marrubium vulgare

    African Journals Online (AJOL)

    SERVER

    2007-11-26

    . Clin. Exp. Hyprtens.26(6): 465-474. Pelczar MJ, Chan ECS, Krieg NR (1993). Microbiology, Int. Ed.Mcgraw. Hill, New York, p. 578. Robert B, Henry T (1880). Medicinal Plants, J & A Churchill, New. Burlington Street, London ...

  12. Das Primat der Politik in kaiserlicher Armee, Reichswehr, Wehrmacht und Bundeswehr

    OpenAIRE

    Dietz, Andreas

    2011-01-01

    Das Primat der Politik in kaiserlicher Armee, Reichswehr, Wehrmacht und Bundeswehr : rechtliche Sicherungen der Entscheidungsgewalt über Krieg und Frieden zwischen Politik und Militär. - Tübingen : Mohr Siebeck, 2011. - XXVII, 780 S. - (Jus publicum ; 210). - Zugl.: Augsburg, Univ., Habil.-Schr., 2010/11

  13. Producción y propiedades de alginato de Sargassum sinicola (Setchell y gardner) y su aplicación en inmovilización celular

    OpenAIRE

    Yabur Pacheco, Ricardo

    2005-01-01

    Se llevó a cabo la producción de alginato de sodio del alga café S. sinicola como material para la inmovilización de la microalga Chlorella sorokiniana y co-inmovilización con la bacteria promotora de crecimiento de plantas, Azospirillum brasilense, para el tratamiento de aguas residuales. El alginato también se empleó para la formulación de microesferas de A. brasilense, para la inoculación de semillas de tomate, como promotores de crecimiento de plantas. Durante la producción se analizaron ...

  14. Plant Growth Promoting Rhizobacteria

    Indian Academy of Sciences (India)

    IAS Admin

    Crop parameters. Rhizobium leguminosarum. Direct growth promotion of canola and lettuce. Pseudomonas putida. Early developments of canola seedlings, growth stimulation of tomato plant. Azospirillum brasilense andA. irakense. Growth of wheat and maize plants. P. flurescens. Growth of pearl millet, increase in growth, ...

  15. Role of bioinoculants in development of salt-tolerance of Vicia faba ...

    African Journals Online (AJOL)

    Through biological inoculation technology, the bacterial-mycorrhizal-legume tripartite symbiosis in saline conditions was documented and the effects of dual inoculation with Azospirillum brasilense (NFB) and Arbuscular mycorrhizal (Am) fungus Glomus clarum on the host plants (Vicia faba) in pot cultures were investigated ...

  16. „Geschichte des Mitmachens“ oder Opferdiskurs. Frauen als Autorinnen von Kriegsliteratur

    Directory of Open Access Journals (Sweden)

    Astrid Gehrig

    2008-03-01

    Full Text Available Cordula Mahr ermöglicht mit ihrer Untersuchung von 70 Autobiographien deutscher Frauen einen Blick auf die „Heimatfront“, ein Blick, der bisher in einer vornehmlich männlich besetzten Kriegsliteratur, die Krieg in erster Linie als Fronterlebnis definiert, vernachlässigt wurde. Die Autorin konzentriert sich auf die Passagen der Autobiographien, die sich direkt auf den Zweiten Weltkrieg beziehen, und berücksichtigt ausschließlich nach 1960 erschienene Texte. In den Darstellungen überwiegt in Bezug auf den Krieg und den Nationalsozialismus das Selbstverständnis als Geschädigte und Leidtragende. Nur sieben Frauen schreiben in ihren Autobiographien auch ihre „Geschichte des Mitmachens”. Mahrs Untersuchung gibt gerade durch diese Auffälligkeit Anlass, auch über formale Strukturmerkmale der Autobiographie nachzudenken, z. B. darüber, ob diese eventuell eine Selbstdarstellung als Opfer begünstigen.

  17. A Noção de “fórmula” em Análise do Discurso: quadro teórico e metodológico

    Directory of Open Access Journals (Sweden)

    Pedro Ivo Silveira Andretta

    2012-12-01

    Full Text Available KRIEG-PLANQUE, Alice. A noção de “fórmula” em análise do discurso: quadro teórico e metodológico. Tradução de Luciana S. Salgado e Sírio Possenti. São Paulo: Parábola Editorial, 2010. (Série Lingua[gem] 39.

  18. Rezension von: Bettina Engels, Corinna Gayer (Hg.: Geschlechterverhältnisse, Frieden und Konflikt. Feministische Denkanstöße für die Friedens- und Konfliktforschung. Baden-Baden: Nomos Verlag 2011.

    Directory of Open Access Journals (Sweden)

    Heinz-Jürgen Voß

    2012-11-01

    Full Text Available Die feministische Friedens- und Konfliktforschung steht vor neuen Herausforderungen, u. a. dadurch, dass Kriege aktuell insbesondere durch Verweis auf Frauen- und Homosexuellenrechte gerechtfertigt werden. Die Autor/-innen in dem von Bettina Engels und Corinna Gayer herausgegebenen Band wenden sich diesen Herausforderungen zu. Neben einer klaren Analyse bieten die Beiträge auch Anregungen für feministische Antworten gegen die ‚feindliche Übernahme‘.

  19. On 23 March ESA’s third Automated Transfer Vehicle (ATV), named in honour of Amaldi, was launched on board an Ariane rocket.

    CERN Multimedia

    CERN Video Productions

    2012-01-01

    Live webcast from CERN on the occasion of the launch of a "Space Ferry", named after Edoardo Amaldi, by the European Space Agency (ESA). Amaldi was CERN's first Secretary General and founding father, and a visionary pioneer for ESA. With the participation of Ugo Amaldi, CERN physicist and son of Edoardo Amaldi, Carlo Rubbia, Nobel Laureate in Physics and Former Director General of CERN and Arturo Russo, historian and author with John Kriege of CERN and ESA's History

  20. Capabilities for Joint Analysis in the Department of Defense: Rethinking Support for Strategic Analysis

    Science.gov (United States)

    2016-01-01

    RR1051.html Lostumbo, Michael J., Michael J. McNerney, Eric Peltz, Derek Eaton, David R. Frelinger, Victoria A. Greenfield, John Halliday, Patrick Mills ...draw on earlier research for two under secretaries for USD(AT&L), Michael Wynne and Kenneth Krieg. Stuart Johnson and James Bexfield provided very... Stuart H. Starr Electrical engineering 2009 Dr. Thomas L. Allen Economics and operations research 2008 Mr. Walter F. Hollis Mathematics, physics 2007 Dr

  1. Research Program in Tropical Infectious Diseases

    Science.gov (United States)

    1994-02-10

    Ph.D.; Jose A. Lopez, M.D.; Llewellyn J. Legters, M.D.; Richard E. Krieg, Ph .). 7. PERFORMING ORGANIZATION NAME(S) AND ADORESS(ES) 8. PERFORMING...W.B. Saunders Co., Phila., PA. 282-297. 12. Organizacion Panamericana de la Salud . 1989. XXXIV Reunion. Situacion de los programas de malaria en las...chlorophyll a concentration); water con- (Orloci 1978) based on the absolute distance dis- ductivity; pH ; and dissolved oxygen. Water anal- similarity

  2. „… höher als die Liebe zur Wissenschaft steht die Treue zum eigenen Vaterland …“: Hallenser Romanisten im Ersten Weltkrieg

    Directory of Open Access Journals (Sweden)

    Annette Schiller

    2014-12-01

    Full Text Available Die 1914 verbreitete Kriegsbegeisterung nahm auch die Romanisten nicht aus. Briefe und Tagebuchnotizen aus der Zeit zeigen, wie Lehrende und Studenten unseres Faches, deren gemeinsamer Bezug die Zugehörigkeit zum Romanischen Seminar Halle war, vom „Großen Krieg“ betroffen waren und führen uns die Situation und Geisteshaltung der Professoren und Studenten und die Rückwirkungen des Krieges auf das Fach vor Augen.

  3. Pharmacology and rationale for imatinib in the treatment of scleroderma

    OpenAIRE

    Moinzadeh, Pia; Krieg,; Hunzelmann,

    2013-01-01

    Pia Moinzadeh, Nicolas Hunzelmann, Thomas Krieg Department of Dermatology and Venerology, University of Cologne, Germany Abstract: Systemic sclerosis (scleroderma) is a chronic, multisystem, fibrotic disease. Although the pathogenesis is not completely understood, early vascular damage leads to an inflammatory reaction and a severe fibrotic response. Therapy of systemic sclerosis is still not convincing and is mainly restricted to the management of organ complications. A wide choice of immun...

  4. „Tace coi morti il monte“: Raumdarstellungen in der italienischen Literatur der Alpenfront

    Directory of Open Access Journals (Sweden)

    Martina Meidl

    2016-06-01

    Full Text Available Der Beitrag stellt Ausschnitte aus der italienischen Literatur der Grande Guerra vor, in denen das Gebirge der Alpenfront als Projektionsraum eines übersteigerten Vitalismus, als Anziehungspunkt einer Sehnsucht nach Sublimität und Transzendenz, als Ort ethischer und sozialer Werte oder als Schauplatz traumatischer Kriegserfahrungen inszeniert wird. Im Kontext des erlittenen Krieges erscheint die Landschaft nicht nur als lebens-, sondern auch identitätsraubender Raum.

  5. Private Military Firms as Instruments of U.S. Foreign Policy: The Case of Columbia

    Science.gov (United States)

    2007-06-01

    Report for Congress, updated November, 2006, p.6. 63 Singer, Corporate Warriors, p.206. 64 Cornelius Friesendorf “Drogen, Krieg und Drogenkrieg- Die...June 13, 2007. 84 Daniel Burton-Rose and Wayne Madsen, “Corporate Soldiers: The U.S. Government Privatizes the Use of Force,” Multinational Monitor...Rose, Daniel and Madsen, Wayne . “Corporate Soldiers: The U.S. Government Privatizes the Use of Force.” Multinational Monitor, March 1999, 20, 3

  6. Plant Cell Protolytic Enzymes Activity under Exposure to Lectins of Endophytic and Epiphytic Azospirillum Strains

    Directory of Open Access Journals (Sweden)

    S.A. Alen’kina

    2016-05-01

    Full Text Available We studied the ability of lectins isolated from the surface of the two strains of nitrogen-fixing soil bacteria of the genus Azospirillum, A. brasilense Sp7 (epiphytic and A. brasilense Sp245 (endophytic, to show have a regulating effect on the activity of pectinolytic enzymes in the roots of wheat seedlings. Research results showed that the lectins under study can cause the induction of the activity of polygalacturonase, pectinesterase, pectatlyase from the plant cell wall, thereby ensuring the bacteria penetration in the plant tissues, as well as the induction of plants responses which, being combined with growth-stimulating effect of bacteria, contributes to the formation of plants stability and productivity.

  7. Increased Growth of the Microalga Chlorella vulgaris when Coimmobilized and Cocultured in Alginate Beads with the Plant-Growth-Promoting Bacterium Azospirillum brasilense†

    Science.gov (United States)

    Gonzalez, Luz E.; Bashan, Yoav

    2000-01-01

    Coimmobilization of the freshwater microalga Chlorella vulgaris and the plant-growth-promoting bacterium Azospirillum brasilense in small alginate beads resulted in a significantly increased growth of the microalga. Dry and fresh weight, total number of cells, size of the microalgal clusters (colonies) within the bead, number of microalgal cells per cluster, and the levels of microalgal pigments significantly increased. Light microscopy revealed that both microorganisms colonized the same cavities inside the beads, though the microalgae tended to concentrate in the more aerated periphery while the bacteria colonized the entire bead. The effect of indole-3-acetic acid addition to microalgal culture prior to immobilization of microorganisms in alginate beads partially imitated the effect of A. brasilense. We propose that coimmobilization of microalgae and plant-growth-promoting bacteria is an effective means of increasing microalgal populations within confined environments. PMID:10742237

  8. Plant-microorganism-soil interactions influence the Fe availability in the rhizosphere of cucumber plants.

    Science.gov (United States)

    Pii, Youry; Penn, Alexander; Terzano, Roberto; Crecchio, Carmine; Mimmo, Tanja; Cesco, Stefano

    2015-02-01

    Iron (Fe) is a very important element for plants, since it is involved in many biochemical processes and, often, for the low solubility of the natural Fe sources in soil, plants suffer from Fe - deficiency, especially when grown on calcareous soils. Among the numerous plant growth-promoting rhizobacteria (PGPR) that colonize the rhizosphere of agronomically important crops, Azospirillum brasilense has been shown to exert strong stimulating activities on plants, by inducing alterations of the root architecture and an improvement of mineral nutrition, which could result from an enhancement of ion uptake mechanisms as well as by increased bioavailability of nutrients. Some studies have also established that A. brasilense can act as biocontrol agent, by preventing the growth and/or virulence of phytopathogens, most likely through the production of microbial siderophores that sequester Fe from the soil. Despite microbial siderophores complexed with Fe could be an easily accessible Fe source for plants, the possible involvement of A. brasilense in improving Fe nutrition in plants suffering from the micronutrient deficiency has not been investigated yet. Within the present research, the characterization of the physiological and biochemical effects induced by Fe starvation and PGPR inoculation in cucumber plants (Cucumis sativus L. cv. Chinese Long) was carried out. The analyses of root exudates released by hydroponically grown plants highlighted that cucumber plants respond differently depending on the nutritional status. In addition, following the cultivation period on calcareous soil, also the root exudates found in the extracts suggested a peculiar behaviour of plants as a function of the treatment. Interestingly, the presence of the inoculum in soil allowed a faster recovery of cucumber plants from Fe-deficiency symptoms, i.e. increase in the chlorophyll content, in the biomass and in the Fe content of leaves. These observations might suggest a feasible application of

  9. Identification and characterization of trans-3-hydroxy-l-proline dehydratase and Δ1-pyrroline-2-carboxylate reductase involved in trans-3-hydroxy-l-proline metabolism of bacteria

    Directory of Open Access Journals (Sweden)

    Seiya Watanabe

    2014-01-01

    Full Text Available trans-4-Hydroxy-l-proline (T4LHyp and trans-3-hydroxy-l-proline (T3LHyp occur mainly in collagen. A few bacteria can convert T4LHyp to α-ketoglutarate, and we previously revealed a hypothetical pathway consisting of four enzymes at the molecular level (J Biol Chem (2007 282, 6685–6695; J Biol Chem (2012 287, 32674–32688. Here, we first found that Azospirillum brasilense has the ability to grow not only on T4LHyp but also T3LHyp as a sole carbon source. In A. brasilense cells, T3LHyp dehydratase and NAD(PH-dependent Δ1-pyrroline-2-carboxylate (Pyr2C reductase activities were induced by T3LHyp (and d-proline and d-lysine but not T4LHyp, and no effect of T3LHyp was observed on the expression of T4LHyp metabolizing enzymes: a hypothetical pathway of T3LHyp → Pyr2C → l-proline was proposed. Bacterial T3LHyp dehydratase, encoded to LhpH gene, was homologous with the mammalian enzyme. On the other hand, Pyr2C reductase encoded to LhpI gene was a novel member of ornithine cyclodeaminase/μ-crystallin superfamily, differing from known bacterial protein. Furthermore, the LhpI enzymes of A. brasilense and another bacterium showed several different properties, including substrate and coenzyme specificities. T3LHyp was converted to proline by the purified LhpH and LhpI proteins. Furthermore, disruption of LhpI gene from A. brasilense led to loss of growth on T3LHyp, d-proline and d-lysine, indicating that this gene has dual metabolic functions as a reductase for Pyr2C and Δ1-piperidine-2-carboxylate in these pathways, and that the T3LHyp pathway is not linked to T4LHyp and l-proline metabolism.

  10. Alternative rooting induction of semi-hardwood olive cuttings by several auxin-producing bacteria for organic agriculture systems

    OpenAIRE

    M. C. Montero-Calasanz; Santamaría, C.; M. Albareda; Daza, A.; Duan, J.; Glick, B. R.; Camacho, M.

    2013-01-01

    Southern Spain is the largest olive oil producer region in the world. In recent years organic agriculture systems have grown exponentially so that new alternative systems to produce organic olive cuttings are needed. Several bacterial isolates, namely Pantoea sp. AG9, Chryseobacterium sp. AG13, Chryseobacterium sp. CT348, Pseudomonas sp. CT364 and Azospirillum brasilense Cd (ATCC 29729), have been used to induce rooting in olive semi-hardwood cuttings of Arbequina, Hojiblanca and Picual culti...

  11. HONGO ENDOMICORRÍZICO Y BACTERIA FIJADORA DE NITRÓGENO INOCULADAS A Coffea arabica EN VIVERO

    Directory of Open Access Journals (Sweden)

    Juan Francisco Aguirre-Medina

    2011-01-01

    Full Text Available Con el objetivo de determinar el efecto de la inoculación con Azospirillum brasilense y Glomus intraradice en café, se estableció la presente investigación en un vivero en el Campo Experimental Rosario Izapa, Chiapas, México de septiembre de 2005 a abril de 2006. Se utilizó un suelo Andosol¿mólico típico de la región cafetalera del Soconusco Chiapas, México. El A. brasilense tuvo una concentración de 100 x 106 bacterias por gramo de turba y el hongo micorrízico, cuarenta esporas por gramo de suelo con infección de 95% en el sistema radical de cebolla. Los tratamientos utilizados fueron los microorganismos solos, la combinación de ambos y el testigo sin inocular que se distribuyeron en un diseño de bloques completos al azar. Se evaluaron variables morfológicas y fisiológicas del rendimiento cada treinta días a partir del segundo mes de siembra durante siete meses y se determinó el contenido de N, P y Ca en el tejido vegetal. Los resultados se analizaron estadísticamente y las diferencias entre tratamientos se compararon de acuerdo a Tukey 5%. Los resultados indican una respuesta diferencial entre los microorganismos. A. brasilense indujo mayor desarrollo radical y la simbiosis doble G. intraradices + A. brasilense mejoró el desarrollo del tallo y lámina foliar. El contenido de N, P y Ca se presentó de forma consistente con G. intraradices.

  12. Polihidroxialcanoatos de cepas de Azospirillum spp. aisladas de raíces de Lycopersicon esculentum Mill. “tomate” y Oryza sativa L. “arroz” en Lambayeque

    Directory of Open Access Journals (Sweden)

    Katty Baca

    2010-01-01

    Full Text Available En este trabajo se determinó la concentración de polihidroxialcanoatos (PH As de cepas de Azospirillum aisladas de raíces de Lycopersicon esculentum Mill. “tomate” y Oryza sativa L. “arroz”, como una alternativa ante la acumulación de plásticos derivados del petróleo. R aíces previamente desinfectadas se sembraron en medio Nfb se misólido, donde las bacterias fijadoras de nitrógeno se reconocieron por una película blanquecina bajo la superficie y el viraje del indicador al azul. El género Azospirillum se identificó en medio rojo de Congo, obteniéndose 96 cepas de A. lipoferum y A. brasilense en tomate y arroz. Se realizó una fermentación discontinua con caldo Azotobacter modificado, alimentando con una solución saturada de ácido málico cada 12 horas y se realizaron tinciones con Sudán Negro B. Se seleccionaron las cepas con mayor nú mero de gránulos de PHAs (en tomate , 18 de A. lipoferum y 2 de A. brasilense y en arroz, 10 de A. lipoferum y 10 de A. brasilense y se cuantificó la biomasa y PHAs. La concentración de PHAs alcanzó 0 . 661 gL - 1 en A. lipoferum KM(T - 73 y 0 . 738 gL - 1 en A. br asilense KM(T - 19. Las cepas de A. lipoferum y A. brasilense aisladas de tomate alcanzaron una mayor concentración de biomasa y PHAs frente a las cepas aisladas de arroz.

  13. The use of bacteria for detecting toxic effects of pollutants in soil and water

    Science.gov (United States)

    Obiakor, Maximilian; Wilson, Susan; Tighe, Matthew; Pereg, Lily

    2017-04-01

    Microbial abundance and diversity are essential for sustaining soil structure and function and have been strongly linked to human health and wellbeing. Antimony (Sb) in the environment can present an ecological hazard and depending on concentration can be lethal. The toxic effects of Sb(III) and Sb(V) on the model soil bacterium Azospirillum brasilense Sp7 were assessed in exposure-dose-response assays and water samples from an Sb contaminated creek were analyzed for bacterial mortality. In both cases, Sb(III) and Sb(V) greatly affected the survival of A. brasilense Sp7 cells. The Sb(III) had a greater toxic effect than Sb(V) at all concentrations tested. Critical concentrations of Sb also caused variant colonies to appear, indicating both acute and sub-lethal effects, which were dose and time dependent. This work demonstrates the usefulness of A. brasilense as an indicator species to detect harmful effects of an environmental pollutant of emerging concern.

  14. Assessing the Effect of Organic Compounds, Biofertilizers and Chemical Fertilizers on Morphological Properties,yield and Yield Components of Forage Sorghum (Sorghum bicolor

    Directory of Open Access Journals (Sweden)

    A.H Saeidnejad

    2012-12-01

    Full Text Available Recently, using the source of organic fertilizers and biofertilizers in sustainable crop production is growing. In order to evaluate the effect of organic compounds, biofertilizers and chemical fertilizer on morphological properties, yield and yield components of forage Sorghum (sorghum bicolor a field experiment was conducted in the Research Farm, College of Agriculture, Ferdowsi University of Mashhad in 2008.The treatments were seed inoculation with the combination of Azotobacter chroococcum and Azospirillum brasilense, Compost (15 t/ha, Vermicompost (10 t/ha, seed inoculation with Azotobacter and Azospirillum and compost (10t/ha, seed inoculation with Azotobacter chroococcum and Azospirillum brasilense and Vermicompost (7t/ha, seed inoculation with Pseudomonas flurescence, seed inoculation with Pseudomonas flurescence and Azotobacter chroococcum and Azospirillum brasilense combination, seed inoculation with Pseudomonas flurescence and compost (15t/ha, chemical fertilizer (80 kg/h urea fertilizer and 50 kg/h super phosphate fertilizer and control. Harvesting was performed in 2 cuts in flowering stage. Plant height, number of tiller per plant and SPAD reading was significantly affected by the treatments. Stem diameter was not affected by any treatments. There was a significant difference among all treatments in terms of fresh and dry forage yield. There were no significant differences among all treatments in terms of stem and leaf dry matter. In general, result of this experiment indicated that organic amendments and biofertilizers could be acceptable alternatives for chemical fertilizers.

  15. A novel interaction between plant-beneficial rhizobacteria and roots: colonization induces corn resistance against the root herbivore Diabrotica speciosa.

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    Franciele Santos

    Full Text Available A number of soil-borne microorganisms, such as mycorrhizal fungi and rhizobacteria, establish mutualistic interactions with plants, which can indirectly affect other organisms. Knowledge of the plant-mediated effects of mutualistic microorganisms is limited to aboveground insects, whereas there is little understanding of what role beneficial soil bacteria may play in plant defense against root herbivory. Here, we establish that colonization by the beneficial rhizobacterium Azospirillum brasilense affects the host selection and performance of the insect Diabrotica speciosa. Root larvae preferentially orient toward the roots of non-inoculated plants versus inoculated roots and gain less weight when feeding on inoculated plants. As inoculation by A. brasilense induces higher emissions of (E-β-caryophyllene compared with non-inoculated plants, it is plausible that the non-preference of D. speciosa for inoculated plants is related to this sesquiterpene, which is well known to mediate belowground insect-plant interactions. To the best of our knowledge, this is the first study showing that a beneficial rhizobacterium inoculant indirectly alters belowground plant-insect interactions. The role of A. brasilense as part of an integrative pest management (IPM program for the protection of corn against the South American corn rootworm, D. speciosa, is considered.

  16. INOCULATION OF DIAZOTROPHIC BACTERIA AND NITROGEN FERTILIZATION IN TOPDRESSING IN IRRIGATED CORN

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    VANESSA ZIRONDI LONGHINI

    2016-01-01

    Full Text Available Corn is a nitrogen-intensive crop, and the use of management practices such as inoculation of the seed with diazotrophic bacteria, which can maximize crop productivity and reduce the need of nitrogen fertilizers, may result in lower production costs. The present study aimed to evaluate the effect of inoculation of corn seed with Azospirillum brasilense and controlled addition of nitrogen to topdressing on the nutrition, production components, and productivity of crop grain. The experimental design was a randomized block design, with four replications in a 2 × 5 factorial scheme. The treatments consisted of inoculation or not of corn seed with A. brasilense (at 100 mL per 25 kg of seed and five nitrogen (N levels in topdressing (0, 30, 60, 90, and 120 kg N ha-1 from urea [45% N] were applied when the corn was in the phenological growth stage V6. Foliar macronutrients, foliar chlorophyll index (FCI, production components, and yield of corn grain were valuated. Inoculation of corn seeds with A. brasilense increased plant height and grain yield. Fertilization in topdressing, with N levels up to 120 kg ha-1, linearly increased the foliar nutrients and productivity of corn cultivated in the spring/summer in the low-altitude Cerrado region of Brazil.

  17. Microorganisms associated to tomato seedlings growing in saline culture act as osmoprotectant

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    Daniel Cortés-Jiménez

    2014-06-01

    Full Text Available Less than 0.5% of total water in the world is available for human consumption and agriculture. The major part of the world's water is saline and salinity in soils interferes in germination of seeds and the posterior development of the plant. In order to increase the osmotolerance of tomato, seedlings were associated with Azospirillum brasilense Cd, Azospirillum brasilense Cd transformed bacteria with a plasmid harboring a trehalose biosynthesis gene-fusion or Chlorella vulgaris. Two plant culture media: Hydroponic and Murashige and Skoog were tested. In the first set of studies seedlings were associated to single free cells meanwhile in a second set single and combined free cells were studied. A positive interaction between transformed Azospirillum and Chlorella vulagris and tomato plants was observed. Seedlings showed a salt concentration tolerance, as sodium chloride, up to 200 mM. According to our results, the association of plants with A. brasilense Cd-BIF and C. vulgaris is a viable approach to increase their salt tolerance and biomass, as consequence the possible use of sea water to irrigate horticultural plants.

  18. Policey, Biopolitik und Liberalismus. Vom Zugriff der Macht auf das Leben (Bios

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    Wolfgang Neurath

    2012-06-01

    Full Text Available Wenn Politik die Fortsetzung des Krieges mit anderen Mitteln ist (Foucault, dann formuliert die moderne Biopolitik neue Standards und Normen in der Regierung des Einzelnen und des Lebens ganzer Populationen. Dieser Artikel untersucht diese historische Entwicklung der Erziehung bzw. der Zivilisierung, die späterhin im Sinne der Züchtung des Lebens zum Kennzeichen der Modernität im Sinne aufgeklärter Gouvernementalität wurde, um dann in den modernen (NeoLiberalismus einzugehen.

  19. Kritik af Krigens Fornuft

    DEFF Research Database (Denmark)

    Harste, Gorm

    Krig har siden 1500-tallet udviklet sig til et selvtilstrækkeligt og selvreferentielt system, der, som Clausewitz viser, kan udgøre fortsættelsen af politik, men i et andet medium. Det medium af kamp og konflikt har historisk uddifferentieret sig og er blevet formet afkoblet fra det politiske sys...... Luhmanns systemteori og udgør for så vidt et svar på det værk Luhmann ikke skrev om Der Krieg der Gesellschaft....

  20. Some critical remarks about new and rare to Polish flora desmids from the Suwałki Lakeland

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    Grażyna M. Tomaszewicz

    2014-01-01

    Full Text Available Species of desmids found in the Suwałki Lakeland are presented; these species are new and rare in the flora of Poland: Cosmarium contractum var. retusum (W. et G. S. West W. Krieg. et Gerl., C. hammeri var. protuberans W. et G. S. West, C. plicatum Reinsch var. plicatum, C. pseudoconnatum var. pseudoconnatum f. latior Borge, Staurastrurim gemelliparum Nordst., S. tohopekaligense var. trifurcatum W. et G. S. West. A critical view is expressed on the creation of infraspecific units within some of these species.

  1. Essays on Strategy 6

    Science.gov (United States)

    1989-01-01

    politics. Ludendorff , however, the so-called "faithful disciple" of Clausewitz, did not see eye to eye with his teacher on this point. He claimed, " War ...the total meaning of war as a ", ntinuation of politics" so as to correctly handle the relationship between war and politics, than to pose as a lover of... Ludendorff , Der Totale Krieg, p.10. 10. Clausewitz, p. 410. 11. Ibid., p. 119. 1.Ibid., p. 121. 1.See "The World Strategy of Social-Imperialism of Soviet

  2. Power, structures, and norms

    OpenAIRE

    Steinel, Anna

    2007-01-01

    Die vorliegende Arbeit beschäftigt sich mit der Untersuchung der institutionalisierten Beziehungen zwischen der NATO und Russland seit 1997 und konzentriert sich dabei besonders auf die Ergebnisse des „Permanent Council“ (PJC, 1997) und des „NATO-Russia Council“ (NRC, 2002). Das Vermächtnis von Strukturen, die während des kalten Krieges entstanden, beeinflusst weiterhin die Interaktionen zwischen der NATO und Russland. Dies wurde insbesondere während der Kosovo-Krise und nach dem 11. Septe...

  3. Sobre Clausewitz, Aron, Raymond : Buenos Aires, Ediciones Nueva Visión, 2009. 155 páginas. ISBN: 978-950-602-589-2.

    OpenAIRE

    Ricardo J. Laleff Ilieff

    2011-01-01

    La figura de Clausewitz ha sido objeto de interés de distintos analistas de la guerra y la teoría política. Así pues, el escritor de Vom Kriege se convirtió en un tópico de referencia para abordar problemáticas fundantes de la modernidad. En esta oportunidad, el lector se encontrará con una recopilación de trabajos que vieron la luz en conferencias y en revistas especializadas de Francia, Inglaterra y Alemania. En este sentido, el presente ejemplar complementa o apoya muchas de las tesis que ...

  4. Review: Dorothée Ninck Gbeassor, Heidi Schär Sall, David Signer, Daniel Stutz & Elena Wertli (1999). Überlebenskunst in Übergangswelten: ethnopsychologische Betreuung von Asylsuchenden

    OpenAIRE

    Hegner, Victoria

    2002-01-01

    Das vorliegende Buch ist das erste von bisher zwei erschienenen Büchern des Ethnologisch-Psychologischen Zentrums in Zürich. In der Einrichtung der Asyl-Organisation für den Kanton Zürich finden Asylsuchende in schweren Krisen stationäre oder ambulante Betreuung. Die Flüchtlinge, die hierher kommen, sind dem Krieg, der Folter und/oder dem Gefängnis in der einstigen Heimat physisch entkommen. Die Migration und allmähliche Ankunft im kulturell und sozial Neuen stellt für sie jedoch eine Überfor...

  5. Demokratieförderung und die Legitimität amerikanischer Außenpolitik: aus amerikanischen Fachzeitschriften, 2. Halbjahr 2004

    OpenAIRE

    Greve, Patricia; Kolkmann, Michael

    2005-01-01

    Gut eineinhalb Jahre nach dem Irak-Krieg hat sich in den USA die wissenschaftliche Debatte über die amerikanische Politik der Demokratieförderung intensiviert. Dabei gibt die instabile Lage im Nachkriegs-Irak Anlaß, die Politik von Präsident George W. Bush kritisch zu beleuchten und nach der grundsätzlichen Legitimität amerikanischer Politik zu fragen. Zugleich wird darüber diskutiert, welche außenpolitische Strategie die Bush-Administration in ihrer zweiten Amtszeit verfolgen wird und verfol...

  6. Critique of the War Reason

    DEFF Research Database (Denmark)

    Harste, Gorm

    was soldier and prisoner of war from age 15-17, would not write a “Der Krieg der Gesellschaft”. Yet the attempt to narrow this lacuna is indeed a heavy burden and a difficult task, in which, firstly, it is methodologically decisive to get the basic distinctions right about a second order observation of war....... Conflict is basically a problem of essentially contested communication. Once this historical self-reference was established around the 17th century, war, thirdly, became delimited by its structural couplings to religion, mass media (propaganda), finance, welfare for victims and veterans, law, politics...

  7. Leadership at the Short End of the Bayonet: The Direction of Leader Training at the U.S. Army Ranger School

    Science.gov (United States)

    1990-12-30

    the natural and inspired precursors of the Blit:krieg principles which we’e later codified by CaptaL •B .H. Liddell Hart i," England .... Pommel’s...ni-. enp iiaceom&,t aod use of cr’ @- , " ..ed .*e eao:.l. ,’ eCO ,3ljfS nC’. obsta:l* brem.:hing. and LLA , l .uual ties were feceS1a’y o* mllSLOM

  8. En torno a la esencia del poder. Un estudio comparativo entre Max Scheler y José Ortega y Gasset

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    Ricardo Gibu Shimabukuro

    2015-01-01

    Full Text Available Ortega’s admiration for Scheler ’s renowned and distinctive way of thinking can be contrasted with his harsh criticism of the relationship between life and war presented in Der Genius des Krieges und der deutsche Krieg (1915. «Life» is not just the struggle for growth and power. It is also characterised by believing and trusting in the permanence of things, in the capacity of disassociating oneself from nature and of taking refuge in our own intimacy and in our will for transforming nature from a world drawn from imagination. The development of Scheler ’s arguments will allow us to redefine life through the action of a principle that overcomes it: that of the «spirit». With his latter statements, several aspects of Ortega’s criticism are overcome. A matter of comparing and contrasting both philosophers’ view on the specificity of human life thus emerges. Our purpose is to approach this issue with the notion of «power».

  9. EFECTO DE LA BIOFERTILIZACIÓN SOBRE EL CRECIMIENTO EN MACETA DE PLANTAS DE CAÑA DE AZÚCAR (Saccharum officinarum EFEITO DA BIOFERTILIZAÇÃO NO CRESCIMENTO DE PLANTAS EM VASOS açúcar de cana (Saccharum officinarum EFFECT OF BIOFERTILIZATION ON THE GROWTH OF POTTED SUGARCANE PLANTS (Saccharum officinarum

    Directory of Open Access Journals (Sweden)

    LILIANA SERNA-COCK

    2011-12-01

    Full Text Available El uso de microorganismos como fertilizante, ha demostrado tener efectos benéficos sobre el crecimiento de plantas y son una alternativa al uso de fertilizantes guímicos, sin embargo, cada microorganismo difiere en sus efectos benéficos. En este trabajo se evaluó el efecto de la aplicación de microorganismos fertilizantes, Azospirillum brasilense, Azotobacter chroccocum y Trichoderma lignorum sobre el crecimiento en maceta de plantas de caña de azúcar variedad CC 934418. El crecimiento de las plantas se midió en términos de diámetro del tallo, longitud de tallo y raíces, y número de hojas y raíces a los 15, 30 y 45 días de la siembra. El crecimiento de las plantas mostró diferencias estadísticamente significativas entre los tratamientos. Los microorganismos fertilizantes mostraron efecto positivo sobre el crecimiento de plantas de caña de azúcar, siendo Azospirillum brasilense y Trichoderma lignorum los microorganismos gue ejercieron mayor efecto sobre el diámetro del tallo y los sistemas radical y foliar de la planta. Se observaron los efectos beneficiosos de Trichoderma lignorum sobre el crecimiento de la hoja. Este es un nuevo aporte científico, ya que esta especie no ha sido reportada como promotora de crecimiento vegetal.0 uso de microrganismos como fertilizante, tem sido demonstrado gue têm efeitos benéficos no crescimento das plantas e são considerados uma alternativa ao uso de fertilizantes guímicos, no entanto, cada microrganismo possui diferentes efeitos benéficos. Neste estudo foi avaliado o efeito da aplicação de microorganismos fertilizantes, Azospirillum brasilense, Azotobacter chroccocum e Trichoderma lignorum no crescimento de cana-de-açucar da variedade CC 934418 plantadas em vasos. 0 crescimento das plantas foi medido em termos do diâmetro do caule, comprimento de caule e da raiz e número de folhas e raízes nos dias 15, 30 e 45 após a semeadura. 0 crescimento da planta mostrou diferen

  10. Seeds inoculation with diazotrophic bacteria and nitrogen application in side-dressing and leaf in maizeInoculação de sementes com bactéria diazotrófica e aplicação de nitrogênio em cobertura e foliar em milho

    Directory of Open Access Journals (Sweden)

    Claudinei Kappes

    2013-05-01

    Full Text Available Considering the importance of nitrogen management and its biological fixation with diazotrophic bacteria, this study was carried out aiming to evaluate the agronomic performance of maize, in response to seed inoculation with Azospirillum brasilense and nitrogen application in side-dressing and leaf. The experiment was conducted in Selvíria, Mato Grosso of Sul State, Brazil, during the growing season 2010/2011, on a clayey Rhodic Haplustox (20º 20’ S and 51º 24’ W, with altitude of 340 m. Sixteen treatments were established with four replications, in randomized blocks with the combination of the factors A. brasilense (with and without inoculante, nitrogen rate (0 and 90 kg ha-1, in V5 growth stage and urea leaf application (0, 4, 8 and 12%: application in V5 and V8 growth stage. The maize hybrid used was the DKB 390 YG®, sowed in the row spacing of 0.9 m. Parameters measured were productive and morphological components of culture and crop yield. Increase in maize yield by seed inoculation with A. brasilense was observed. The application of 90 kg ha-1 of nitrogen in side-dressing provided higher chlorophyll leaf index, stalk diameter and prolificacy, however, the yield not was increased. The application of urea leaf did not agronomic efficiency and, therefore, should not be used as the unique form of supply and alternative to nitrogen addition to crop. Considerando a importância do manejo do nitrogênio e da sua fixação biológica através de bactérias diazotróficas, conduziu-se este trabalho com o objetivo de avaliar o desempenho agronômico do milho, em função da inoculação das sementes com Azospirillum brasilense e da aplicação de nitrogênio em cobertura e foliar. O experimento foi conduzido no município de Selvíria – MS, durante o ano agrícola 2010/2011, sob Latossolo Vermelho distrófico típico argiloso (20º 20’ S e 51º 24’ W, com altitude de 340 m. Foram estabelecidos dezesseis tratamentos com quatro repeti

  11. Plant growth-promoting bacteria associated with nitrogen fertilization at topdressing in popcorn agronomic performance

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    Leandro Teodoski Spolaor

    2016-03-01

    Full Text Available ABSTRACT The use of plant growth-promoting bacteria is a promising alternative with low environmental impact to increase the efficiency of use of chemical fertilizers, ensuring high yield with better cost-effective ratio. In maize crops, several studies have demonstrated an increased yield when Azospirillum-based inoculants are used. In the case of popcorn, there are no available studies related to use of inoculation and its response on yield parameters. Thus, the aim of this study was to evaluate the field performance of popcorn when inoculated with the commercial product Masterfix L (A. brasilense Ab-V5 and A. brasilense Ab-V6 and the non-commercial inoculant UEL (A. brasilense Ab-V5 + Rhizobium sp. 53GRM1 associated with nitrogen fertilization. The trials were conducted in Londrina and Maringá, Paraná State, Brazil, in a randomized block design with four replications, in a split plot design with the inoculation treatments located in the plots (uninoculated, Masterfix L, and UEL and the different N rates located in the subplots where ammonium sulphate was applied in the topdressing at the V6 stage (0, 50, 100, and 150 kg∙ha–1. The variance analysis showed significant effects (p < 0.05 of inoculation (Londrina environment and N rates (both environments only for grain yield. There was no inoculation effect in the grain yield when inoculants were applied together with N-fertilization at topdressing. In the absence of N-fertilization at topdressing, the inoculants Masterfix L. and UEL promoted higher grain yield as compared to the uninoculated plants, with resulting increases of 13.21 and 26.61% in yield, respectively.

  12. EFECTO DE LA BIOFERTILIZACIÓN SOBRE EL CRECIMIENTO EN MACETA DE PLANTAS DE CAÑA DE AZÚCAR (Saccharum officinarum) EFEITO DA BIOFERTILIZAÇÃO NO CRESCIMENTO DE PLANTAS EM VASOS açúcar de cana (Saccharum officinarum) EFFECT OF BIOFERTILIZATION ON THE GROWTH OF POTTED SUGARCANE PLANTS (Saccharum officinarum)

    OpenAIRE

    LILIANA SERNA-COCK; CAMILO ARIAS-GARCÍA; LEIDY JOHANA VALENCIA HERNANDEZ

    2011-01-01

    El uso de microorganismos como fertilizante, ha demostrado tener efectos benéficos sobre el crecimiento de plantas y son una alternativa al uso de fertilizantes guímicos, sin embargo, cada microorganismo difiere en sus efectos benéficos. En este trabajo se evaluó el efecto de la aplicación de microorganismos fertilizantes, Azospirillum brasilense, Azotobacter chroccocum y Trichoderma lignorum sobre el crecimiento en maceta de plantas de caña de azúcar variedad CC 934418. El crecimiento de las...

  13. Characterization of a Major Cluster of nif, fix, and Associated Genes in a Sugarcane Endophyte, Acetobacter diazotrophicus

    Science.gov (United States)

    Lee, Sunhee; Reth, Alexander; Meletzus, Dietmar; Sevilla, Myrna; Kennedy, Christina

    2000-01-01

    A major 30.5-kb cluster of nif and associated genes of Acetobacter diazotrophicus (syn. Gluconacetobacter diazotrophicus), a nitrogen-fixing endophyte of sugarcane, was sequenced and analyzed. This cluster represents the largest assembly of contiguous nif-fix and associated genes so far characterized in any diazotrophic bacterial species. Northern blots and promoter sequence analysis indicated that the genes are organized into eight transcriptional units. The overall arrangement of genes is most like that of the nif-fix cluster in Azospirillum brasilense, while the individual gene products are more similar to those in species of Rhizobiaceae or in Rhodobacter capsulatus. PMID:11092875

  14. GERMINATION OF GRASSES DUE TO INOCULATION DIAZOTROPHIC BACTERIA

    Directory of Open Access Journals (Sweden)

    C. D. A. Moreira

    2014-07-01

    Full Text Available The germination of forage grasses suffers from numbness and a natural tendency to low quality. The use of microorganisms inoculated in seeds with the purpose of increasing and meet the demand of some nutrient has been shown to be efficient, but the role of the microorganism in germination and rate of force is still unknown. Therefore the goal as study was to evaluate the germination rate of seeds of three cultivars of Brachiaria brizantha CV. Marandu, b., b. brizantha CV. Xaraés and b. humidícola cv Tupi and a cultivar of millet, P. hybrid cv Massai depending on the bacterium Azospirillum brasilense diazotrofic inoculation (nitrogen-fixing. Germination test was used in seed dispersal to assess the effect of first count (VPC in the treatments with and without inoculation. It was done also conducted further tests of electrical conductivity, weight of thousand seeds and water content. The delineation used was randomized entirely (DIC and the statistical analysis carried out through the analysis of variance and comparison of means using the Tukey test, the 5% probability. Massai grass seeds have the highest rate of force of first count in both treatments. Inoculation of bacterium Azospirillum brasilense did not affect the values of force of first count on seeds of the cultivars Marandu, Xaraés, Tupi and Massai. The seeds of the massai have higher germination speed relative the other cultivars evaluated when inoculated.

  15. Cellulose Decomposition and Associated Nitrogen Fixation by Mixed Cultures of Cellulomonas gelida and Azospirillum Species or Bacillus macerans

    Science.gov (United States)

    Halsall, Dorothy M.; Gibson, Alan H.

    1985-01-01

    Mixed cultures of Cellulomonas gelida plus Azospirillum lipoferum or Azospirillum brasilense and C. gelida plus Bacillus macerans were shown to degrade cellulose and straw and to utilize the energy-yielding products to fix atmospheric nitrogen. This cooperative process was followed over 30 days in sand-based cultures in which the breakdown of 20% of the cellulose and 28 to 30% of the straw resulted in the fixation of 12 to 14.6 mg of N per g of cellulose and 17 to 19 mg of N per g of g straw consumed. Cellulomonas species have certain advantages over aerobic cellulose-degrading fungi in being able to degrade cellulose at oxygen concentrations as low as 1% O2 (vol/vol) which would allow a close association between cellulose-degrading and microaerobic diazotrophic microorganisms. Cultures inoculated with initially different proportions of A. brasilense and C. gelida all reached a stable ratio of approximately 1 Azospirillum/3 Cellulomonas cells. PMID:16346898

  16. Maize response to inoculation with strains of plant growth-promoting bactéria

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    Janaína Dartora

    Full Text Available ABSTRACT The aim of this study was to evaluate the response of maize to inoculation with strains of plant growth-promoting bacteria (PGPB in two cultivation years. The experiment was set in a randomized block design with four replicates in two cultivation years (2012/13 and 2013/14. The treatments consisted of PGPB inoculation: control (without N and without inoculation; 30 kg of N ha-1 at sowing (N1; 160 kg of N ha-1 (N1 + 130 kg of N ha-1 as top-dressing; N1 + A. brasilense, Ab-V5; N1 + A. brasilense, HM053; N1 + Azospirillum sp. L26; N1 + Azospirillum sp. L27; N1 + Enhydrobacter sp. 4331; N1 + Rhizobium sp. 8121. Basal stem diameter, plant height, leaf area, shoot dry matter and yield were evaluated. The strain of Rhizobium sp. 8121and the isolate Azospirillum sp. L26 associated with 30 kg of N ha-1 at sowing promoted yields equivalent to that of the N fertilization of 160 kg ha-1, demonstrating the potential to be used in the inoculation of maize seeds.

  17. Rapid quantification of rice root-associated bacteria by flow cytometry.

    Science.gov (United States)

    Valdameri, G; Kokot, T B; Pedrosa, F de O; de Souza, E M

    2015-03-01

    To understand the mechanism of plant-bacterium interaction, it is critical to enumerate epiphytic bacteria colonizing the roots of the host. We developed a new approach, based on flow cytometry, for enumerating these bacteria and used it with rice plants, 7 and 20 days after colonization with Herbaspirillum rubrisubalbicans and Azospirillum brasilense. The results were compared with those obtained with the traditional plate count method. Both methods gave similar numbers of H. rubrisubalbicans associated with rice roots (c. 10(9) CFU g(-1) ). However, flow cytometry gave a number of viable cells of rice-associated A. brasilense that was approx. 10-fold greater than that obtained with the plate count method. These results suggest that the plate count method can underestimate epiphytic populations. Flow cytometry has the additional advantage that it is more precise and much faster than the plate count method. Determination of precise number of root-associated bacteria is critical for plant-bacteria interaction studies. We developed a flow cytometry approach for counting bacteria and compared it with the plate count method. Our flow cytometry assay solves two major limitations of the plate count method, namely that requires long incubation times of up to 48 h and only determines culturable cells. This flow cytometry assay provides an efficient, precise and fast tool for enumerating epiphytic cells. © 2014 The Society for Applied Microbiology.

  18. Unexpected phytostimulatory behavior for Escherichia coli and Agrobacterium tumefaciens model strains.

    Science.gov (United States)

    Walker, Vincent; Bruto, Maxime; Bellvert, Floriant; Bally, René; Muller, Daniel; Prigent-Combaret, Claire; Moënne-Loccoz, Yvan; Comte, Gilles

    2013-05-01

    Plant-beneficial effects of bacteria are often underestimated, especially for well-studied strains associated with pathogenicity or originating from other environments. We assessed the impact of seed inoculation with the emblematic bacterial models Agrobacterium tumefaciens C58 (plasmid-cured) or Escherichia coli K-12 on maize seedlings in nonsterile soil. Compared with the noninoculated control, root biomass (with A. tumefaciens or E. coli) and shoot biomass (with A. tumefaciens) were enhanced at 10 days for 'PR37Y15' but not 'DK315', as found with the phytostimulator Azospirillum brasilense UAP-154 (positive control). In roots as well as in shoots, Agrobacterium tumefaciens and E. coli triggered similar (in PR37Y15) or different (in DK315) changes in the high-performance liquid chromatography profiles of secondary metabolites (especially benzoxazinoids), distinct from those of Azospirillum brasilense UAP-154. Genome sequence analysis revealed homologs of nitrite reductase genes nirK and nirBD and siderophore synthesis genes for Agrobacterium tumefaciens, as well as homologs of nitrite reductase genes nirBD and phosphatase genes phoA and appA in E. coli, whose contribution to phytostimulation will require experimental assessment. In conclusion, the two emblematic bacterial models had a systemic impact on maize secondary metabolism and resulted in unexpected phytostimulation of seedlings in the Azospirillum sp.-responsive cultivar.

  19. Victor F. Weisskopf (1908 - 2002): Physikgeschichte

    Science.gov (United States)

    Jacobi, Manfred

    2002-11-01

    Universal gebildete und interessierte Menschen sind selten geworden in unserer Zeit. Victor F. Weisskopf war einer von ihnen. Sein geistiger Horizont umfasste nicht nur sein eigentliches Fachgebiet, die Physik, wo er sich durch fundamentale Arbeiten in den Bereichen der Quantenfeldtheorie, der Kern- und Elementarteilchenphysik hervortat. Daneben bildeten Kunst und Musik sowie ein außergewöhnliches Engagement in sozialen und politischen Angelegenheiten die Eckpunkte seines Lebens. Der Einsatz für die Verständigung zwischen den Machtblöcken während des Kalten Krieges war für ihn ebenso selbstverständlich wie das Bedürfnis, die neuen Ideen der Physik einem breiten Publikum nahe zu bringen. Die Wissenschaft erschien ihm als das geeignete Mittel, um zum Wohle der Menschheit zu wirken.

  20. Qualidade de Vida: uma fórmula?

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    Marcela Franco Fossey

    2013-01-01

    Full Text Available O presente trabalho aborda materiais diversos com orientações sobre como as pessoas podem ter um estilo de vida mais saudável. Uma reflexão preliminar sobre esses materiais permite apontar como colocam em evidência uma característica bastante proeminente das sociedades modernas, especialmente as ocidentais: a necessidade de cuidar do corpo social. Permite, igualmente, identificar alguns sintagmas cujo funcionamento parece ser tipicamente formulaico (Krieg-Planque, 2010, 2011, o que seria um forte indício de que ser saudável e ter uma longa vida, no mundo ocidental contemporâneo, está relacionado com um funcionamento discursivo bastante complexo.

  1. Duelo, imagen (post) expresionista e intertexto bíblico: una interpretación de “Iglesia abandonada (Balada de la Gran Guerra)”, de Federico García Lorca

    OpenAIRE

    Llera, José Antonio

    2015-01-01

    El artículo aborda una interpretación en clave comparatista de uno de los poemas de la sección segunda de Poeta en Nueva York, incidiendo en el concepto de duelo proveniente del psicoanálisis y en las imágenes que componen la serie de grabados de Otto Dix, Der Krieg. Además, explora su imaginario religioso a través de sus vínculos intertextuales con los textos bíblicos, así como las convergencias temáticas con la poesía expresionista alemana y con otras narraciones de la I Guerra Mundial, com...

  2. How to Keep our Youth away from IS: The Need for Narrative Analysis and Strategy

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    Bob de Graaff

    2015-10-01

    Full Text Available The German war theorist Carl von Clausewitz pointed out that in order to defeat an opponent one had to crush his point of gravitation (Schwerpunkt.[1] In the case of non-state opponents, like the Islamic State, which do occupy territory, but more or less in a nomadic way, moving like a snake among the rocks, it is difficult to target a physical point of gravitation. In fact their Schwerpunkt is their ideological narrative, which functions as a redoubtable pull-factor, responsible for attracting throngs of foreign fighters. So far the West has shown a poor record in targeting this narrative. This paper tries to map some of the elements in IS’s Schwerpunkt that look vulnerable and may be targeted in order to diminish the power of the Islamic State as much as possible without actually fighting the self-proclaimed Caliphate. [1] http://www.clausewitz.com/readings/VomKriege1832/TOC.htm.

  3. Sexuelle Gewalt in Besatzungsalltag und Vernichtungskrieg

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    Silke Schneider

    2005-03-01

    Full Text Available Als Grundlage für ihre Analyse des Umgangs der Wehrmacht mit sexueller Gewalt im Zweiten Weltkrieg dienen Birgit Beck die Akten militärgerichtlicher Verfahren. Das Thema wird von der Autorin in die Geschichte der sexuellen Gewalt in Kriegen von der Frühen Neuzeit bis zur Gegenwart eingeordnet. Ziel ist die Überprüfung geschichts- und sozialwissenschaftlicher Annahmen über die Ursachen sexueller Gewalt im Krieg am konkreten historischen Beispiel. Die Gründe für die militärgerichtliche Verfolgung sexueller Gewalt – die nur einen Bruchteil der Verfahren insgesamt darstellt – sieht Beck in erster Linie in übergeordneten militärischen Verhaltensvorgaben, dem Männerbild der Wehrmacht und dem Schutz der deutschen „Volksgemeinschaft“ begründet; für einen systematischen Einsatz sexueller Gewalt durch die Wehrmacht bieten die untersuchten Verfahren keine ausreichenden Belege.

  4. [Peter von Brackel. Christlich Gesprech von der grawsamen Zerstörung in Lifland durch den Muscowiter vom 58. Jar her geschehenn...] / Stefan Donecker

    Index Scriptorium Estoniae

    Donecker, Stefan, 1977-

    2015-01-01

    Arvustus: Christlich Gesprech von der grawsamen Zerstörung in Lifland durch den Muscowiter vom 58. Jar her geschehenn: auch ihren Ursachen mit einer kurtzen Predig und Vermanung, wie beid, Gotlosenn unnd Frommen, diese schreckliche Mutation fruchtbarlich behertzigen und ihnen zu Nutz machen sollen: durch Timannum Brakel Livoniensem, der Gemeine Christi vonn der Augsburgischen Confession Prediger zu Anttorf einfeltig gestellet unnd inn Druck verfertiget = Darstellung der Geschichte Livlands vor und während des "Livländischen Krieges" bis 1578 in gereimter Dialogform und Prosa von dem Prediger und Geschichtsschreiber Timann Brakel : Originaltext und Übersetzung ins Hochdeutsche mit Kommentaren, Ergänzungen, Bildern und dem Lebenslauf des Timann Brakel. (Beiträge zur baltischen Geschichte, 19). Verlag Harro von Hirschheydt. Wedemark 2012

  5. Critique of War Reason. A Perspective on Self-referential Systems, 11th-21st Centuries

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    Gorm Harste

    2016-03-01

    Full Text Available This paper is a summary of my 700-page very academic thesis, in Danish, to be published by Aarhus University Press (AUP. A shorter booklet based on it was published by AUP too (November 2014, 250 pages and so were a number of shorter articles in English, French and German. In Luhmann’s systems theory and in sociology at large there is a missing link consisting in the lack of a sociology of war. A number of German systems theoreticians use Luhmann’s theory to fill that gap. Yet Luhmann (born 1927, who was a soldier and a prisoner of war from age 15-17, would not write a “Der Krieg der Gesellschaft”. The attempt to narrow this lacuna is indeed a heavy burden and a difficult task, in which it is decisive firstly to get the basic distinctions right about a second order observation of war as a conflict system – to be distinct from a military organisational system. This, I do by beginning with a reconceptualization of Carl von Clausewitz’ form analysis and self-description of war from Vom Kriege (1832. The central point is to observe the self-reference of war, or how war became war about war. Conflict is basically a problem of essentially contested communication. Once this historical self-reference established around the 17th century was in place, war became delimited by its structural couplings to religion, mass media (propaganda, finance, welfare for victims and veterans, law, politics and other functional systems. The costs of war increased, reconstituted and transformed modern society in a way that has formed a range of risks and – of course – neglected blind spots.

  6. „Geschichte des Mitmachens“ oder Opferdiskurs. Frauen als Autorinnen von Kriegsliteratur “History of Participation” or Victim Discourse: Women as Authors of War Literature

    Directory of Open Access Journals (Sweden)

    Astrid Gehrig

    2008-03-01

    Full Text Available Cordula Mahr ermöglicht mit ihrer Untersuchung von 70 Autobiographien deutscher Frauen einen Blick auf die „Heimatfront“, ein Blick, der bisher in einer vornehmlich männlich besetzten Kriegsliteratur, die Krieg in erster Linie als Fronterlebnis definiert, vernachlässigt wurde. Die Autorin konzentriert sich auf die Passagen der Autobiographien, die sich direkt auf den Zweiten Weltkrieg beziehen, und berücksichtigt ausschließlich nach 1960 erschienene Texte. In den Darstellungen überwiegt in Bezug auf den Krieg und den Nationalsozialismus das Selbstverständnis als Geschädigte und Leidtragende. Nur sieben Frauen schreiben in ihren Autobiographien auch ihre „Geschichte des Mitmachens”. Mahrs Untersuchung gibt gerade durch diese Auffälligkeit Anlass, auch über formale Strukturmerkmale der Autobiographie nachzudenken, z. B. darüber, ob diese eventuell eine Selbstdarstellung als Opfer begünstigen.Cordula Mahr’s examination of 70 autobiographies of German women provides insight into the “home front,” insight that till now has been neglected in male-dominated war literature, which defines war first and foremost as an experience of the front. The author concentrates on passages in autobiographies that refer to the World War II directly and takes into account only those texts that appeared before 1960. Self-images as the marred and the suffering woman with respect to war and to National Socialism dominate the descriptions. Only seven women include their own “history of participation” in their autobiographies. Mahr’s study also incites a consideration of the formal structural features of the autobiography, for example, whether the structures may potentially aid the construction of a self-portrait as victim.

  7. Kinematografische Propaganda und Zensur in Österreich-Ungarn von 1914–1918 als gescheitertes kybernetisches Modell

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    Paul Winkler

    2014-12-01

    Full Text Available (Medien-Pädagogik, verstanden als Medienverbund zur programmierten "Unterrichtung" einer breiten Masse wurde bereits vor 1914 erfahren. Zweifelsohne kam es jedoch während dem Ersten Weltkrieg zu einer intensiven Orchestrierung der zur Verfügung stehenden Medien. Die angestrebte Volkserziehung kann dabei als kybernetisches Regelkreismodell betrachtet werden. Als ganz spezielles systemimmanentes Instrument, stellte sich dabei das Kino heraus. Anfangs etwas zögerlich, verstand man es mit Verlauf des Krieges auch in der Donaumonarchie, die Möglichkeiten der bewegten Bilder immer umfassender auszunützen. Das Kino sollte als Regler, dem Publikum Führungsgrößen vor Augen führen und das einzelne Individuum gleichzeitig zu einer Art Selbstregulation auffordern. Vorstellungs- und Wertewelten der Menschen sollten homogenisiert werden und das System in dieser Weise stabilisieren. Offenes Feedback war unerwünscht; Grundstimmungen und Diskurse innerhalb der Bevölkerung blieben jedoch nicht unbemerkt und wurden auf der Leinwand bearbeitet, wobei begründete Befürchtungen, Zweifel und Ängste, zu entkräften versucht wurden. Mit fortschreitender Kriegsdauer taten sich Zensur und Propaganda immer schwerer, ein geschöntes Bild der Situation glaubhaft darzustellen. Schließlich vermochte das Flimmern der Leinwände, die Botschaften des Systems nicht mehr zu vermitteln. Das kybernetische Modell der Volkserziehung über Kamera und Leinwand, welches die Gesellschaft militärisch überprägte, Überlegenheit predigte, Gewalt normalisierte und das kriegsbedingte Sterben humanisierte, musste letztlich scheitern. Der vorliegende Artikel soll einen kurzen Einblick in den Aufbau dieses – letztlich gescheiterten – regulativen Werkes der kinematografischen Propaganda und Zensur in Österreich-Ungarn geben, wobei der Fokus auf der dokumentarischen Berichterstattung vom Krieg liegt, die eine vermeintliche Realität repräsentieren sollte.

  8. Massenmedien und Versöhnung: Die Berichterstattung über den deutsch-französischen Friedensprozeß nach dem 2. Weltkrieg

    Directory of Open Access Journals (Sweden)

    Susanne Jaeger

    2003-10-01

    Full Text Available Der vorliegende Beitrag beinhaltet die ersten Ergebnisse einer Langzeitstudie, bei der flächendeckend die deutsche Nachkriegsberichterstattung über Frankreich von 1946 bis 1970 inhaltsanalytisch ausgewertet wurde. Die Studie stützt sich dabei auf das Modell der Nachrichtenfaktoren von Johan Galtung, welche nach seiner Ansicht die Auswahl der Themen bestimmen, die zu einer Nachricht werden. Genau in diesen Selektionsroutinen liegt jedoch die implizite Gefahr, Konflikte zu vertiefen statt sie einzudämmen bzw. durch ein breites Verständnis der Hintergründe gewaltfrei bearbeitbar zu machen. Für die Zeit nach einem Krieg könnten sie Hindernisse auf dem Weg zur Annäherung und Aussöhnung ehemaliger Gegner darstellen. Wie jedoch sieht die Berichterstattung nach dem Krieg tatsächlich aus? Zeigen Massenmedien Bereitschaft zu einer veränderten Berichterstattung? Der Fall der französisch-deutschen Aussöhnung, welcher als Beispiel für einen gelungenen Aussöhnungsprozess gelten kann, belegt, dass Friedensprozesse von den Medien durchaus adäquat begleitet werden können: Z.B. ist der Anteil "positiver" Themen bzw. Berichterstattung konsistent höher als der "negativer", und der Anteil von Non-Elite-Themen steigt an und kündet von Interesse an französischer Lebensart und Kultur. Die Ergebnisse zeigen, dass Negativismus in den Medien kein Naturgesetz ist, sondern überwunden werden kann, wenn Frieden und Versöhnung auf der Tagesordnung stehen.

  9. SOILS, FERTILIZATION AND MANAGEMENT OF WATER Halotolerant/alkalophilic bacteria associated with the cyanobacterium Arthrospira platensis (Nordstedt Gomont that promote early growth in Sorghum bicolor (L. Moench

    Directory of Open Access Journals (Sweden)

    Liliana Gómez G

    2012-01-01

    Full Text Available Arthrospira platensis associated bacteria (APAB identified through molecuar biology like Bacillus okhensis, Indibacter alkaliphilus and Halomonas sp., are also producing 3-indol acetic acid (IAA, these bacteria was used in early plant growth promotion tests over Sorghum bicolor, these bioassay was considered indirect evidence to suggest that APAB also may have stimulatory effects over A. platensis growth naturally. I. alkaliphilus and B. okhensis enhanced early germination of S. bicolor seads, with better results than that achieved by Azospirillum brasilense, bacterium used like reference as a common plant growth promoting rizobacteria. The three APAB enhanced significative differences (P≤0.05 over morphoagronomic parameters, I. alkaliphilus and B. okhensis exhibith better resoults in elongation stimulation and root and foliage dry weight. Above evidence suggest this bacteria like plant growth promoting and it recomended testing with A. platensis axenic cultures and its associated bactteri for understanding true interaction between them.

  10. Ocorrência e diversidade de bactérias diazotróficas associadas a gramíneas forrageiras do Pantanal Sul Matogrossense Occurrence and diversity of diazotrophic bacteria associated to forage grasses of the Pantanal in the state of Mato Grosso do Sul

    Directory of Open Access Journals (Sweden)

    Marivaine da Silva Brasil

    2005-04-01

    Full Text Available Este trabalho teve por objetivo avaliar a ocorrência e a diversidade genética de bactérias fixadoras de N2 associadas às gramíneas nativas Elyonurus muticus (capim carona e Axonopus purpusii (capim mimoso e à gramínea exótica Brachiaria humidicola (braquiária que formam as pastagens na região da Nhecolândia. As coletas das plantas e solo foram feitas nos períodos de seca e de cheia para determinar a população de bactérias diazotróficas. Identificaram-se Azospirillum brasilense, A. lipoferum, A. amazonense, Herbaspirillum spp., Burkholderia spp. em amostras de solo, raízes e folhas das três espécies forrageiras. As populações dessas bactérias foram menores na época da cheia em comparação com a época da seca. A diversidade genética das bactérias isoladas foi avaliada por meio da técnica de análise de restrição do DNA ribossomal amplificado (ARDRA. Os isolados foram divididos em cinco grupos genotípicos distintos. Os isolados de A. brasilense e A. lipoferum apresentaram cerca de 50 % de similaridade, enquanto A. amazonense formou um grupo a parte, com apenas 25 % de similaridade em relação ao grupo das espécies do gênero. As bactérias do gênero Herbaspirillum formaram um grupo isolado com apenas 25 % de similaridade em relação ao gênero Azospirillum. O quinto grupo foi formado por apenas um isolado com 25 % de similaridade em relação aos demais.This study was carried out to verify the occurrence and genetic diversity of diazotrophic bacteria associated to the native graminaceus plants Elyonurus muticus (Carona grass and Axonopus purpusii (Mimoso grass and the exotic Brachiaria humidicola (Brachiaria that form the pastures in the Nhecolândia region of the Pantanal in the state of Mato Grosso do Sul, Brazil. The plants were harvested in the dry and rainy seasons and the diazotrophic bacteria populations in the soil and on roots and leaves of the three grasses were determined. Strains belonging to the

  11. Molecular responses in root-associative rhizospheric bacteria to variations in plant exudates

    Science.gov (United States)

    Abdoun, Hamid; McMillan, Mary; Pereg, Lily

    2015-04-01

    Plant exudates are a major factor in the interface of plant-soil-microbe interactions and it is well documented that the microbial community structure in the rhizosphere is largely influenced by the particular exudates excreted by various plants. Azospirillum brasilense is a plant growth promoting rhizobacterium that is known to interact with a large number of plants, including important food crops. The regulatory gene flcA has an important role in this interaction as it controls morphological differentiation of the bacterium that is essential for attachment to root surfaces. Being a response regulatory gene, flcA mediates the response of the bacterial cell to signals from the surrounding rhizosphere. This makes this regulatory gene a good candidate for analysis of the response of bacteria to rhizospheric alterations, in this case, variations in root exudates. We will report on our studies on the response of Azospirillum, an ecologically, scientifically and agriculturally important bacterial genus, to variations in the rhizosphere.

  12. AMPLIFICATION OF AZOSPIRILLUM SP. JG3 GLPD GENE FRAGMENT USING DEGENERATE PRIMERS GENERATED BY WEB-BASED TOOLS

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    Stalis Norma Ethica

    2013-12-01

    Full Text Available Primaclade and In Silico web-based tools were used as a strategy to obtain the correct-size PCR amplicon targeting a fragment of gene encoding glycerol-3-phosphate dehydrogenase (glpD of Azospirillum sp. JG3. The bacterial strains are soil, Gram-negative PGPR (Plant-Growth Promoting Rhizobacteria isolated from an agricultural land in Purwokerto, Central Java, Indonesia, which have ability to produce several commercial enzymes. The aim is to obtain a pair of reliable degenerate primers from a limited number of glpD sequences from other Azospirilla retrieved in GenBank using bioinformatics approach. We demonstrated degenerate primer design that led to successful PCR amplification corresponding to the targeted DNA fragment. Homology analysis showed that the obtained DNA fragment is 61% and 99% similar to sn-glycerol-3-phosphate dehydrogenase genes of Azospirillum brasilense and Stenotrophomonas maltophili respectively.

  13. A simple and efficient method for poly-3-hydroxybutyrate quantification in diazotrophic bacteria within 5 minutes using flow cytometry

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    L.P.S. Alves

    Full Text Available The conventional method for quantification of polyhydroxyalkanoates based on whole-cell methanolysis and gas chromatography (GC is laborious and time-consuming. In this work, a method based on flow cytometry of Nile red stained bacterial cells was established to quantify poly-3-hydroxybutyrate (PHB production by the diazotrophic and plant-associated bacteria, Herbaspirillum seropedicae and Azospirillum brasilense. The method consists of three steps: i cell permeabilization, ii Nile red staining, and iii analysis by flow cytometry. The method was optimized step-by-step and can be carried out in less than 5 min. The final results indicated a high correlation coefficient (R2=0.99 compared to a standard method based on methanolysis and GC. This method was successfully applied to the quantification of PHB in epiphytic bacteria isolated from rice roots.

  14. A simple and efficient method for poly-3-hydroxybutyrate quantification in diazotrophic bacteria within 5 minutes using flow cytometry.

    Science.gov (United States)

    Alves, L P S; Almeida, A T; Cruz, L M; Pedrosa, F O; de Souza, E M; Chubatsu, L S; Müller-Santos, M; Valdameri, G

    2017-01-16

    The conventional method for quantification of polyhydroxyalkanoates based on whole-cell methanolysis and gas chromatography (GC) is laborious and time-consuming. In this work, a method based on flow cytometry of Nile red stained bacterial cells was established to quantify poly-3-hydroxybutyrate (PHB) production by the diazotrophic and plant-associated bacteria, Herbaspirillum seropedicae and Azospirillum brasilense. The method consists of three steps: i) cell permeabilization, ii) Nile red staining, and iii) analysis by flow cytometry. The method was optimized step-by-step and can be carried out in less than 5 min. The final results indicated a high correlation coefficient (R2=0.99) compared to a standard method based on methanolysis and GC. This method was successfully applied to the quantification of PHB in epiphytic bacteria isolated from rice roots.

  15. Genome-wide identification of microRNA and siRNA responsive to endophytic beneficial diazotrophic bacteria in maize.

    Science.gov (United States)

    Thiebaut, Flávia; Rojas, Cristian A; Grativol, Clícia; Motta, Mariana Romeiro; Vieira, Tauan; Regulski, Michael; Martienssen, Robert A; Farinelli, Laurent; Hemerly, Adriana S; Ferreira, Paulo C G

    2014-09-06

    Small RNA (sRNA) has been described as a regulator of gene expression. In order to understand the role of maize sRNA (Zea mays-hybrid UENF 506-8) during association with endophytic nitrogen-fixing bacteria, we analyzed the sRNA regulated by its association with two diazotrophic bacteria, Herbaspirillum seropedicae and Azospirillum brasilense. Deep sequencing analysis was done with RNA extracted from plants inoculated with H. seropedicae, allowing the identification of miRNA and siRNA. A total of 25 conserved miRNA families and 15 novel miRNAs were identified. A dynamic regulation in response to inoculation was also observed. A hypothetical model involving copper-miRNA is proposed, emphasizing the fact that the up-regulation of miR397, miR398, miR408 and miR528, which is followed by inhibition of their targets, can facilitate association with diazotrophic bacteria. Similar expression patterns were observed in samples inoculated with A. brasilense. Moreover, novel miRNA and siRNA were classified in the Transposable Elements (TE) database, and an enrichment of siRNA aligned with TE was observed in the inoculated samples. In addition, an increase in 24-nt siRNA mapping to genes was observed, which was correlated with an increase in methylation of the coding regions and a subsequent reduction in transcription. Our results show that maize has RNA-based silencing mechanisms that can trigger specific responses when plants interact with beneficial endophytic diazotrophic bacteria. Our findings suggest important roles for sRNA regulation in maize, and probably in other plants, during association with diazotrophic bacteria, emphasizing the up-regulation of Cu-miRNA.

  16. Effect of immobilized rhizobacteria and organic amendment in bulk and rhizospheric soil of Cistus albidus L.

    Science.gov (United States)

    Mengual, Carmen Maria; del Mar Alguacil, Maria; Roldan, Antonio; Schoebitz, Mauricio

    2013-04-01

    A field experiment was carried out to assess the effectiveness of the immobilized microbial inoculant and the addition of organic olive residue. The microbial inoculant contained two rhizobacterial species identified as Azospirillum brasilense and Pantoea dispersa immobilized in a natural inert support. Bacterial population densities were 3.5×109 and 4.1×109 CFU g-1 of A. brasilense M3 and P. dispersa C3, respectively. The amendment used was the organic fraction extracted with KOH from composted "alperujo". The raw material was collected from an olive-mill and mixed with fresh cow bedding as bulking agent for composting. The inoculation of rhizobacteria and the addition of organic residue were employed for plant growth promotion of Cistus albidus L. and enhancement of soil physicochemical, biochemical and biological properties in a degraded semiarid Mediterranean area. One year after planting, the available phosphorus and potassium content in the amended soils was about 100 and 70% respectively higher than in the non-amended soil. Microbial inoculant and their interaction with organic residue increased the aggregate stability of the rhizosphere soil of C. albidus (by 12% with respect to control soil) while the organic residue alone not increased the aggregate stability of the rhizosphere of C. albidus. Microbial biomass C content and enzyme activities (dehydrogenase, urease, protease-BAA and alkaline phosphatase) of the rhizosphere of C. albidus were increased by microbial inoculant and organic residue interaction but not by microbial inoculation alone. The microbial inoculant and organic residue interaction were the most effective treatment for stimulating the roots dry weight of C. albidus (by 133% with respect to control plants) and microbial inoculant was the most effective treatment for increase the shoot dry weigh of plants (by 106% with respect to control plants). The combined treatment, involving microbial inoculant and addition of the organic residue

  17. Alternative rooting induction of semi-hardwood olive cuttings by several auxin-producing bacteria for organic agriculture systems

    Directory of Open Access Journals (Sweden)

    M. C. Montero-Calasanz

    2013-01-01

    Full Text Available Southern Spain is the largest olive oil producer region in the world. In recent years organic agriculture systems have grown exponentially so that new alternative systems to produce organic olive cuttings are needed. Several bacterial isolates, namely Pantoea sp. AG9, Chryseobacterium sp. AG13, Chryseobacterium sp. CT348, Pseudomonas sp. CT364 and Azospirillum brasilense Cd (ATCC 29729, have been used to induce rooting in olive semi-hardwood cuttings of Arbequina, Hojiblanca and Picual cultivars of olive (Olea europea L. The first four strains were previously selected as auxin-producing bacteria and by their ability to promote rooting in model plants. They have been classified on the basis of their 16S rDNA gene sequence. The known auxin producer A. brasilense Cd strain has been used as a reference. The inoculation of olive cuttings was performed in two different ways: (i by dipping cuttings in a liquid bacterial culture or (ii by immersing them in a paste made of solid bacterial inoculant and sterile water. Under nursery conditions all of the tested bacterial strains were able to induce the rooting of olive cuttings to a similar or greater extent than the control cuttings treated with indole-3-butyric acid (IBA. The olive cultivars responded differently depending on the bacterial strain and the inoculation method. The strain that consistently gave the best results was Pantoea sp. AG9, the only one of the tested bacterial strains to express the enzyme 1-aminocyclopropane-1-carboxylate (ACC deaminase. The results are also discussed in terms of potential commercial interest and nursery feasibility performance of these strains.

  18. Identification of nif genes in N2-fixing bacterial strains isolated from rice fields along the Yangtze River Plain.

    Science.gov (United States)

    Xie, Guang Hui; Cui, Zongjun; Yu, Jun; Yan, Jing; Hai, Weili; Steinberger, Yosef

    2006-01-01

    The aim of this research was to identify nifH and nifHDKYE ' genes in twenty strains of N2-fixing heterotrophic bacteria isolated from rice fields in the Yangtze River Plain. Southern hybridization of the total DNA from each strain was performed with the Klebsiella pneumoniae nifHDKYE ' gene probe (6.2 kb Eco RI fragment from pSA30) and the Azospirillum brasilense nifH gene probe (0.6 kb Eco RI-Hin dIII fragment from pHU8). We found that Eco RI fragments of total DNA from Aeromonas hydrophila HY2, Bacillus azotoformans FD, Bacillus licheniformis NCH1, NCH5, WH4, Bacillus brevis NC2, Bacillus pumilus NC12, Bacillus cereus NCH2, Citrobacter freundii HY5, HY9, Derxia gummosa HZ5, Pseudomonas mendocina HZ1 and Pseudomonas pseudoalcaligenes WH3 were positively hybridized with both of the probes. Agrobacterium radiobacter HY17, Corynebacterium sp. HY12, YZ and Pseudomonas sp. HY11 had Eco RI fragments hybridized with the K. pneumoniae nifHDKYE ' gene probe. An Eco RI fragment of total DNA from Bacillus megaterium YY4 was positively hybridized to the A. brasilense nifH gene probe. No hybridization sign was found in the total DNA fragments from Alcaligenes cupidus YY6 and Corynebacterium sp. NC11 hybridized with either of the gene probes. The data provide the number and size of EcoRI fragments of the total DNA hybridized with the nif gene probes for these strains of rarely studied species, suggesting additional evidence for N2 fixing and nif gene diversity of N2-fixing bacteria in rice fields along the Yangtze River Plain. ((c) 2006 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim).

  19. KONY 2012, Military Humanitarianism, and the Magic of Occult Economies KONY 2012, militärische Humanität und die Magie verborgener Wirtschaftsbeziehungen

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    Sverker Finnström

    2012-01-01

    Full Text Available The global success of the film KONY 2012 by Invisible Chil-dren, Inc., manifests far greater magical powers than those of Joseph Kony and his ruthless Lord’s Resistance Army, which it portrays. The most prominent feature of the Invisible Children lobby is the making and constant remaking of a master narrative that depoliticizes and dehistoricizes a murky reality of globalized war into an essentialized black-and-white story. The magic of such a digestible storyline, with Ugandan rebel leader Joseph Kony as a global poster boy for evil personified, not only plays into the hands of the oppressive Ugandan government but has also become handy for the US armed forces as they seek to increase their presence on the African continent. As the US-led war on terror is renewed and expanded, Invisible Children’s humanitarian slogan, “Stop at nothing”, has proven to be exceptionally selective, manifesting the occult economy of global activism that calls for military interventions.Der weltweite Erfolg des Films KONY 2012 von Invisible Children, Inc. offenbart weit stärkere magische Kräfte, als sie dem darin portraitierten Joseph Kony und seiner skrupellosen „Lord’s Resistance Army“ zugeschrieben werden. Die wichtigste Wirkung der Lobbyarbeit von Invisible Children besteht darin, die finstere Realität des globalisierten Krieges zu entpolitisieren, zu enthistorisieren und auf ein immer neu variiertes Schwarz-Weiß-Denkmuster zu reduzieren. Die magische Wirkung einer solchen leicht verwertbaren inhaltlichen Reduktion – mit dem ugandischen Rebellenführer Joseph Kony als globalem Inbegriff des personifizierten Bösen – liegt im Interesse der repressiven ugandischen Regierung, insbesondere aber auch der US-Streitkräfte, die ihre Präsenz auf dem afrikanischen Kontinent verstärken wollen. Während der US-geführte Krieg gegen den Terror wieder aufgenommen und ausgeweitet wird, hat sich der von Invisible Children genutzte Slogan „Stop at

  20. Singapore-India Relations: A Return to History

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    Ming Hwa Ting

    2009-01-01

    Full Text Available Michael Leifer observes Singapore-India relations to be ‘diplomatically distant’. This observation was correct during the Cold War when differing political ideologies made it difficult for these two countries to develop close relations. With the end of the Cold War, bilateral relations improved rapidly, especially on the economic front. Consequently, most literature focuses on the economic interaction between them, at the expense of other significant developments on the political, military as well as social and cultural fronts. In order to better understand Singapore-India relations in the present, a well-rounded approach is necessary. Hence, this article addresses this lacuna in the present scholarship by providing a comprehensive overview that takes into account developments in both the areas of high and low politics. In so doing, this article argues that Singapore-India relations are now no longer ‘diplomatically distant’, but instead mirror the close relations they had during the colonial period, and so represent a ‘return to history’ instead. --- Michael Leifer beschreibt die Beziehungen zwischen Singapur und Indien als "diplomatisch distanziert". Diese Beobachtung war während des Kalten Krieges korrekt, als unterschiedliche politische Ideologien eine enge Zusammenarbeit dieser beiden Länder erschwerten. Mit dem Ende des Kalten Krieges verbesserten sich bilaterale Beziehungen – vor allem im wirtschaftlichen Bereich – rapide. Aus diesem Grund konzentrieren sich die meisten Publikationen auf die wirtschaftliche Interaktion der beiden Länder und vernachlässigen gleichzeitig weitere wichtige Entwicklungen in der politischen, militärischen, sozialen und kulturellen Sphäre. Um die Beziehungen zwischen Singapur und Indien besser zu verstehen, ist ein vielseitigerer Ansatz notwendig. Der vorliegende Artikel versucht diese Lücke gegenwärtiger Forschung zu schließen und einen umfassenden Überblick der Entwicklungen

  1. In situ radiation measurement and estimation of U/Th ratio to reflect on the uranium bearing potential zone in metamorphic rocks of Mahendragarh district, Haryana

    Science.gov (United States)

    Somani, O. P.; Sunil Kumar, T. S.; Kukreti, B. M.; Bhaumik, B. K.; Gorikhan, R. A.

    2009-06-01

    Gross gamma radiation survey was carried out using Nal(Tl) scintillator based portable gamma ray spectrometer (PGRS) around areas of Gaonri, Dholera, Pachnota and Meghot in Mahendragarh district, Haryana. Geologically the area forms part of north Delhi fold belt comprising calc-silicate, quartz biotite schist, impure marble, quartzite and pegmatite rocks. Equivalent uranium (eU3O8) concentration in ppm was estimated in situ on a regular grid pattern of 500 m (E-W) × 1000 m (N-S) and grab samples were collected at grid locations for analyzing in the laboratory for estimating the contents of eU3Os Raeq, ThO2 and %K. A comparison with the laboratory analysed grab samples for eU308 data and in situ radiation measurements shows a good match of the two sets of data. The in situ measurements indicate higher concentration of eU3O8 in Chapra Bibipur in northeastern most corners, Maghot area in central part, Gaonri in western part and Pachnota in southwestern part of the study area. As index to uranium favorability, U(Raeq)-Th contour map (prepared using Surfer software with Krieging interpolation method for this grid size) based on the data on grab samples was generated which show three major clusters of relatively high U-Th ratio. The blocks delineated are enriched in sodic mineral albite which support albite hosted uranium mineralization potential in metamorphic rocks in Haryana.

  2. NATO aggression against FRY: “A war on the border between law and morality”

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    Radojičić Mirjana

    2011-01-01

    Full Text Available The paper focuses on the ethical aspects of NATO aggression (or so-called “humanitarian intervention”, exerted against the FRY, actually Serbia, in the period from March to June 1999. The paper has been conceived as a critical dialogue with Jürgen Habermas, or rather the positions presented by him in a text entitled “Bestialität und Humanität: Eine Krieg an der Grenze zwischen Recht und Moral”. Following a short presentation of Habermas’s point of view, in the introduction to her paper the author discusses the moral implications of, as he points out, “the surgical precision of the air-strikes, as well as the programmatic sparing of the civilian population”, which are, according to him, the characteristics of this aggression with “a highly legitimating effect”. She then focuses on the key and declaratively moral argument that served to justify the aggression - the protection of human rights of Kosmet Albanians and the prevention of the humanitarian catastrophe they had been allegedly exposed to. After analyzing this argument thoroughly, in several steps, the author concludes that the aggression was not, as Habermas claimed, a “war on the border between law and morality”, but that it was actually beyond the borders of both law and morality and was deeply situated into a domain of interest-driven armed acts of the US foreign policy.

  3. Radiant business. Hazards of international, illicit trafficking with nuclear materials; Strahlende Geschaefte. Gefahren des internationalen Atomschmuggels

    Energy Technology Data Exchange (ETDEWEB)

    Attali, J.

    1996-03-01

    Since the Cold War has been terminated, public media increasingly come out with reports about cases of illicit trafficking with nuclear technology and nuclear materials. So far, the potential hazard has not been exploding into the big disaster, but imagine what may happen if uranium or plutonium falls into the hands of terrorists, fanatics, or Mafia-type organisations ? The author has been investigating into this problem on behalf of the Secretary General of the UN. He has been travelling all around the world in pursuit of information and indications, and now presents us with the essential results of his mission, compiled in this explosive report. (orig./HP) [Deutsch] Seit dem Ende des Kalten Krieges berichten die Medien immer haeufiger ueber Schmuggel von Atomtechnologie und spaltbarem Material. Bis heute ist uns eine Katastrophe erspart geblieben. Was geschieht aber, wenn Uran oder Plutonium in die Haende von Terroristen, Fanatikern oder mafiaaehnlichen Kartellen faellt? Der Autor ist im Auftrag des Generalsekretaers der Vereinten Nationen dieser Frage nachgegangen. Rund um die Welt fuehrten ihn seine Nachforschungen und Gespraeche. Die wichtigsten Ergebnisse aus seinem brisanten Bericht enthuellt er in diesem Buch. (orig./HP)

  4. Privatiserung von Geschichte. Probleme einer differenzierten Aufarbeitung

    Directory of Open Access Journals (Sweden)

    Birthe Kundrus

    2000-11-01

    Full Text Available Ausgangspunkt der Untersuchung von Vera Neumann ist die These, daß im Krieg Erlebtes und Erlittenes in den Wiederaufbau- und Wirtschaftswunderjahren Westdeutschlands einem Thematisierungstabu unterlegen sei. Anhand von 50 Interviews, die in den 80er Jahren im Rahmen des von Lutz Niethammer geleiteten Projekts „Lebensgeschichte und Sozialgeschichte im Ruhrgebiet 1930–1960“ (LUSIR entstanden sind und die sie jetzt anhand der Tonbandprotokolle und Abschriften ein zweites Mal auswertet, möchte die Historikerin diese Verschüttungen aufspüren. Im Anschluß an Niethammers These der „Privatisierung von Geschichte“ will sie zeigen, daß seelische und körperliche Kriegsfolgen wie Deprivations- und Überlastungsgefühle, der Verlust von Angehörigen und Kriegsbeschädigungen „privatisiert“, d.h. an die Familien übertragen wurden. Dort seien dann in erster Linie die weiblichen Familienmitglieder mit der Versorgungs- und Pflegearbeit konfrontiert worden. An vier Fallbeispiele schließt sich eine detaillierte Auswertung des gesamten Interviewmaterials an. Diesen Teilen folgt ein Abschnitt zur staatlichen Kriegsopferversorgung in der frühen Bundesrepublik.

  5. O sintagma "progressão continuada" sob a lupa da análise do discurso: um caso de fórmula discursiva?

    Directory of Open Access Journals (Sweden)

    Rosana de Barros Silva e Teixeira

    2014-03-01

    Full Text Available Este artigo apresenta os resultados de uma pesquisa acerca do conceito de fórmula discursiva. Em pauta, está o sintagma "progressão continuada", regime de ciclos implantado em 1998 no estado de São Paulo. Para embasar a análise, os pressupostos teóricos da Análise de Discurso francesa foram eleitos, segundo os quais quatro propriedades incidem na caracterização da fórmula (KRIEG-PLANQUE, 2010: cristalização do significante; caráter discursivo; funcionamento como referente social e aspecto(s polêmico(s. Em adição, o aporte teórico-metodológico da Linguística de Corpus (BERBER SARDINHA, 2004 foi utilizado a fim de proceder à investigação do corpus (de 432.146 palavras. Os dados, provenientes de gêneros discursivos variados, referem-se ao período de 1997 a 2011. Por meio deles, foi possível concluir que "progressão continuada" pode ser considerada uma fórmula, uma vez que os traços que a definem sinalizam sua constituição discursiva.

  6. RADIKALISIERUNGSPROZESSE WESTLICHER DSCHIHADISTEN. EINE UNTERSUCHUNG AM BEISPIEL DENIS CUSPERT

    Directory of Open Access Journals (Sweden)

    Niklas Palm

    2017-03-01

    Full Text Available Diese Arbeit setzt sich mit der Frage auseinander, wie sich westliche Dschihadisten radikalisieren und welche konkreten Faktoren dazu beitragen können. Anhand theoretisch ausgearbeiteter Modelle, soll der Frage am konkreten Beispiel Denis Cuspert nachgegangen werden. Aufgrund der Brisanz des Themas, allein in der Bundesrepublik sind in den letzten Jahren über 850 Personen nach Syrien oder in den Irak ausgereist, liegt die Notwendigkeit einer Auseinandersetzung mit dessen Hintergründen nahe. Gerade hinsichtlich Cuspert, dem die Sicherheitsbehörden ein erhebliches Mobilisierungs- und Gefahrenpotential zusprechen, wurden bisher vor allem nicht-akademische Beiträge gewidmet, weshalb diese Arbeit den Anspruch erhebt, diese zu sammeln und den Radikalisierungsprozess Cusperts in einer theoriegeleiteten, empirischen Untersuchung darzulegen. Die Ergebnisse spiegeln, wie in der Forschung zur Radikalisierung vielfach ersichtlich wird, die Vielschichtigkeit des Prozesses wieder. So sind es, wie so oft, persönliche Krisen und Diskriminierungserfahrungen, Identitätsprobleme, Einflüsse extremistischer Bewegungen sowie Narrative dass der Westen einen Krieg gegen den Islam führt, die letztlich dazu führen, dass er in den Dschihad zieht. Was der Fall Cuspert aber auch lehrt ist, dass sich vor allem aufgrund von individueller Charakteristika erklären lässt, warum in fundamentalistischen Kreisen manch einer zur Waffe greift und ein anderer nicht.

  7. ASPECTOS DE UMA NÃO LEITURA: PROJETOS POLÍTICO-PEDAGÓGICOS E DEMOCRACIA

    Directory of Open Access Journals (Sweden)

    Márcio Antônio Gatti

    2017-04-01

    Full Text Available O objetivo deste estudo é analisar discursivamente as recusas e/ou resistências para a liberação dos Projetos Político-Pedagógicos (PPP durante a coleta de dados que compuseram o corpus de nossa pesquisa em torno do termo “democracia” nos PPP de escolas da rede estadual situadas no município de Sorocaba – SP. Para tanto, baseia-se na noção de fórmula proposta por Krieg-Planque (2010, cujos trabalhos se enquadram na Análise do Discurso de orientação francesa. As análises demonstram que as recusas e/ou resistências são índices do caráter polêmico em torno do termo “democracia” e de seu termo correlato “gestão democrática”. Indicam também que a comunidade escolar é negligenciada no processo de difusão do PPP, de modo que o documento tenha como destinatário apenas os órgãos burocráticos superiores. Conclui-se que a negociação do termo “democracia” afeta diretamente o funcionamento da gestão democrática e do PPP, colocando em discussão a finalidade destes.

  8. One hundred years of the Piston aeroplane engine. Part 2. The period from 1919 to the 1950s; Hundert Jahre Kolbenflugmotor. Teil 2. Die Zeit zwischen 1919 und den 1950er Jahren

    Energy Technology Data Exchange (ETDEWEB)

    Meinig, U. [Hengst GmbH und Co. KG, Muenster (Germany)

    2003-11-01

    To celebrate the centenary of the Wright brothers' legendary first flight, this series of articles has been dedicated to examining the development history of the piston aeroplane engine. Part 2 of three parts focuses on the following issues from a mainly German perspective: the development of piston aeroplane engines against the backdrop of burgeoning commercial and sports aviation in the 1920s and 1930s. The following sections then describe the enforced air armament of Germany in the run-up to World War II, the efforts to increase aeroplane engine performance during the war, and the final stages of development towards the end of the era of large aeroplane engine construction in the late 1950s. (orig.) [German] Aus Anlass des hundertsten Jahrestages des Erstflugs der Gebrueder Wright wird hier die Entwicklungsgeschichte des Kolbenflugmotors beleuchtet. Dieser Teil 2 von drei Teilen widmet sich, mit dem Schwerpunkt auf der Situation in Deutschland, zunaechst der Entwicklung der Kolbenflugmotoren vor dem Hintergrund der aufkeimenden Verkehrs- und Sportfliegerei in den 1920er- und 1930er-Jahren. Anschliessend werden skizziert: die forcierte Luftruestung Deutschlands vor dem Zweiten Weltkrieg, die Anstrengungen zur Steigerung der Leistungsfaehigkeit von Flugmotoren waehrend des Krieges und die letzten Entwicklungsschritte bis zum Ende des Baus von Grossflugmotoren Ende der 1950er-Jahre. (orig.)

  9. Prof. Dr. rer. nat. habil. Günter Hoppe zum 85. Geburtstag

    Directory of Open Access Journals (Sweden)

    H.-J. Bautsch

    2004-01-01

    Full Text Available Die ersten Kontakte mit dem Museum für Naturkunde hatte Günter Hoppe bereits vor über fünfundfünfzig Jahren. Er besuchte Paul Ramdohr, der damals noch, bis zu seiner Berufung im April 1950 nach Heidelberg, Direktor des Mineralogisch-Petrographischen Institutes und Museums war. Zu dieser Zeit was Günter Hoppe Student an der Universität Halle und mit seiner Abschlussarbeit beschäftigt. Sein Lehrer in Halle war Ferdinand von Wolff, noch im hohen Alter Direktor des Mineralogisch-Petrographischen Institutes. Begonnen hatte Günter Hoppe 1938 ein Studium der Chemie in Halle, das durch die Einberufung zum Militärdienst unterbrochen werden musste. Nach Rückkehr aus dem Krieg nahm er mit Beginn des Studienbetriebes an der Universität Halle 1946 sein Studium wieder auf, jetzt aber in der Mineralogie, wohl angeregt durch eine Praktikumstätigkeit in einem Bergbaubetrieb. doi:10.1002/mmng.20040070111

  10. Continuity in a Changing World: Malaysia’s Coercive Security Apparatus in the Age of Terror and Beyond

    Directory of Open Access Journals (Sweden)

    Andrew Humphreys

    2009-01-01

    Full Text Available The Malaysian government’s use of its repressive security legislation has had a significant impact on Malaysia’s modern political history. The focus of the present article is on the government’s use of its coercive security apparatus since the terrorist attacks of 9/11. My argument is that the apparatus is largely unchanged by the current global climate of the "War on Terror." Notably, Malaysia’s use of coercion has become increasingly less criticized by other governments, notably those in the West. Perhaps as a consequence, the government has become increasingly bold in its crackdowns against opposition elements. Following the political upheaval of the 2008 election, however, the future of the security apparatus is in question. --- Der Einsatz repressiver Sicherheitsgesetze durch die malaysische Regierung beeinflusste die moderne politische Geschichte Malaysias in nicht unerheblicher Weise. Dieser Beitrag analysiert den Einsatz des Sicherheitsapparates durch die malaysische Regierung seit den Terroranschlägen des 11. September 2001. Ich argumentiere, dass dieser Apparat im gegenwärtigen weltweiten Klima des "Kriegs gegen den Terrorismus" größtenteils unverändert blieb, dass jedoch gleichzeitig Malaysias Einsatz von Gewalt von anderen (vor allem westlichen Regierungen weniger kritisiert wird als früher. Eine mögliche Konsequenz dessen ist die zunehmend rücksichtslosere Vorgehensweise der Regierung gegen oppositionelle Strömungen. Als Folge des politischen Umschwungs nach der Wahl 2008 steht die Zukunft des Sicherheitsapparates jedoch in den Sternen.

  11. Molecular characterization of nitrogen-fixing bacteria isolated from brazilian agricultural plants at São Paulo state Caracterização molecular de bactérias fixadoras de nitrogênio isoladas de plantas brasileiras no estado de São Paulo

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    Érica. L. Reinhardt

    2008-09-01

    Full Text Available Fourteen strains of nitrogen-fixing bacteria were isolated from different agricultural plant species, including cassava, maize and sugarcane, using nitrogen-deprived selective isolation conditions. Ability to fix nitrogen was verified by the acetylene reduction assay. All potentially nitrogen-fixing strains tested showed positive hybridization signals with a nifH probe derived from Azospirillum brasilense. The strains were characterized by RAPD, ARDRA and 16S rDNA sequence analysis. RAPD analyses revealed 8 unique genotypes, the remaining 6 strains clustered into 3 RAPD groups, suggesting a clonal origin. ARDRA and 16S rDNA sequence analyses allowed the assignment of 13 strains to known groups of nitrogen-fixing bacteria, including organisms from the genera Azospirillum, Herbaspirillum, Pseudomonas and Enterobacteriaceae. Two strains were classified as Stenotrophomonas ssp. Molecular identification results from 16S rDNA analyses were also corroborated by morphological and biochemical data.Quatorze linhagens de bactérias fixadoras de nitrogênio foram isoladas de diferentes espécies de plantas, incluindo cassava, milho e cana-de-açúcar, usando condições seletivas desprovidas de nitrogênio. A capacidade de fixar nitrogênio foi verificada por ensaio de redução de acetileno. Todas as linhagens fixadoras de nitrogênio testadas apresentaram hibridização positiva com sonda de gene nifH derivada de Azospirillum brasilense. As linhagens foram caracterizadas por RAPD, ARDRA e sequenciamento do gene 16S rDNA. As análises de RAPD revelaram 8 genótipos, as 6 linhagens restantes foram agrupadas em 3 grupos de RAPD, sugerindo uma origem clonal. ARDRA e seqüências de 16S rDNA foram alocadas em 13 grupos conhecidos de bactérias fixadoras de nitrogênio, incluindo organismos dos gêneros Azospirillum, Herbaspirillum, Pseudomonas e Enterobacteriaceae. Duas linhagens foram classificadas como Stenotrophomonas ssp. Os resultados da identifica

  12. Evaluación de la asociación bacterias fijadoras de nitrógeno - líneas interespecíficas de arroz-nitrógeno, en Typic haplustalf. Ibagué, Colombia Evaluation of the association nitrogen fixing bacterias interspecific - rice lines - nitrogen, in typic haplustalf. Ibagué, Colombia

    Directory of Open Access Journals (Sweden)

    Margarita M Vallejo

    2008-01-01

    Full Text Available El estudio se llevó a cabo en la hacienda Cauchitos, municipio de Ibagué, departamento del Tolima (Norte 4° 23' 51" y Oeste 75° 9' 7", 979 msnm, 24.3°C, bosque seco tropical (bs-T, con el objetivo de evaluar las asociaciones entre bacterias fijadoras de nitrógeno con inóculo y sin él en diez líneas interespecíficas de arroz, con tres dosis de nitrógeno (0%, 50% y 100% de 250 kg/ha-1 y tres repeticiones por tratamiento. La inoculación se realizó con 1 cm³ de unidades formadoras de colonias por 250 g de semilla de cada cultivar. Se aislaron 2.260 bacterias de los géneros Azotobacter spp y Azospirillum spp, se identificaron las especies A. brasilense, A. lipoferum, A. amazonense y del género Azotobacter las especies A. chroococcum, A. vinelandii, A. paspali y A. beijerinckii. Respecto al inóculo no se encontraron diferencias significativas al realizar su aplicación, se determinó que Azotobacter spp y Azospirillum spp fueron géneros típicos de la flora bacteriana en el cultivo del arroz y en condiciones de campo hubo efecto de los tratamientos en la flora bacteriana, y Azotobacter spp fue el predominante en cada uno de los tratamientos.The study was carried out at the Cauchitos farm, Ibague municipality department of Tolima, with bounds: North 4°23'51" and west 75°9'7", 979 ansm, the average temperature is 24,3°C, tropical dry forest (bs-t in the Holdridge classification. The purpose of this study was to evaluate the association between the nitrogen fixation bacteries with and without inoculo in 10 interespecific rice lines with three nitrogen dosis (0, 50 and 100% de 250 kg/ ha-1 and three repetitions. The inoculation was realized with 10(8 former units of colonies per millimeter. 2.260 bacteries of the generums Azotobacter spp y Azospirrillum spp., and identification the species: Azospirillum brasilense, Azospirillum lipoferum, Azospirillum amazonense, were identified and from the genus Azotobacter were identified the

  13. Growth promoting characteristics of rhizobacteria and AM Fungi for biomass amelioration of Zea mays

    Directory of Open Access Journals (Sweden)

    Kumar Manoj

    2015-01-01

    Full Text Available Plant growth promoting rhizobacteria (PGPR and mycorrhiza were evaluated on the growth (biomass and yield of Zea mays. In the present study, selective rhizospheric PGPR (Azotobacter chroococcum, Pseudomonas aeruginosa, Azospirillum brasilense and Streptomyces sp. and a combination of six strains of arbuscular mycorrhizal fungi (AMF (Acaulospora morrowae, Gigaspora margarita, Glomus constrictum, Glomus mossae, Glomus aggregatum and Scutellospora calospora were isolated and identified with standard methods and 16S rRNA sequence analysis. PGPR and AMF were checked for their growth-promoting behavior under specific treatment conditions. The 30-48-day-old treated plants in all combinations showed a significantly higher mass value. The average dry weight from the shoot was in a range from 41-52% as compared to the control. This increase also translated into a higher mass value of the roots. Overall, an 82% growth rate was observed in terms of height as the consequence of biomass production, specifically in the case of AMF + rhizobacteria combination. We report an efficient, sustainable and cost-effective biofertilizer for enhanced biomass of Z. mays, one of the staple food crops worldwide.

  14. Bioremediation of hydrocarbons contaminating sewage effluent using man-made biofilms: effects of some variables.

    Science.gov (United States)

    Al-Mailem, D M; Kansour, M K; Radwan, S S

    2014-11-01

    Biofilm samples were established on glass slides by submerging them in oil-free and oil-containing sewage effluent for a month. In batch cultures, such biofilms were effective in removing crude oil, pure n-hexadecane, and pure phenanthrene contaminating sewage effluent. The amounts of the removed hydrocarbons increased with increasing biofilm surface area exposed to the effluent. On the other hand, addition of the reducing agent thioglycollate dramatically inhibited the hydrocarbon bioremediation potential of the biofilms. The same biofilm samples removed contaminating hydrocarbons effectively in three successive batch bioremediation cycles but started to become less effective in the cycles thereafter, apparently due to mechanical biofilm loss during successive transfers. As major hydrocarbonoclastic bacteria, the biofilms harbored species belonging to the genera Pseudomonas, Microvirga, Zavarzinia, Mycobacterium, Microbacterium, Stenotrophomonas, Gordonia, Bosea, Sphingobium, Brachybacterium, and others. The nitrogen fixer Azospirillum brasilense and the microalga Ochromonas distigma were also present; they seemed to enrich the biofilms, with nitrogenous compounds and molecular oxygen, respectively, which are known to enhance microbiological hydrocarbon degradation. It was concluded that man-made biofilms based upon sewage microflora are promising tools for bioremediation of hydrocarbons contaminating sewage effluent.

  15. Oviposition Attractancy of Bacterial Culture Filtrates: response of Culex quinquefasciatus

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    S Poonam

    2002-04-01

    Full Text Available Oviposition attractants could be used for monitoring as well as controlling mosquitoes by attracting them to lay eggs at chosen sites. In the present study, culture filtrates of seven bacterial species were tested for their attractancy against gravid females of Culex quinquefasciatus. When their oviposition active indices (OAI were studied, the culture filtrates of Bacillus cereus and Pseudomonas fluorescens exhibited oviposition attractancy (OAI = >0.3 at 100 ppm and the OAI were respectively 0.70 and 0.47. Culture filtrates of B. thuringiensis var. israelensis (wild type, B. t. var. israelensis (mutant and B. sphaericus showed attractancy at 2000 ppm with OAI of respectively 0.71, 0.59 and 0.68. However, the OAI of B. megaterium as well as Azospirillum brasilense was 0.13 (at 2000 ppm, which was less than 0.3 required to be considered them as attractants. When the oviposition attractancy of the bacterial culture filtrates were compared with that of a known oviposition attractant, p-cresol (at 10 ppm, the culture filtrates of B. t. var. israelensis (wild type and B. cereus were found to be more active than p-cresol, respectively with 64.2 and 54.3% oviposition.

  16. Screening plant growth promoting rhizobacteria for improving seed germination, seedling growth and yield of maize.

    Science.gov (United States)

    Nezarat, S; Gholami, A

    2009-01-01

    The effect of Plant Growth-Promoting Rhizobacteria (PGPR) on seed germination, seedling growth and yield of field grown maize were evaluated in three experiments. In these experiments six bacterial strains include P. putida strain R-168, P. fluorescens strain R-93, P. fluorescens DSM 50090, P. putida DSM291, A. lipoferum DSM 1691 and A. brasilense DSM 1690 were used. Results of first study showed seed inoculation significantly enhanced seed germination and seedling vigour of maize. In second experiment, leaf and shoot dry weight and also leaf surface area significantly were increased by bacterial inoculation in both sterile and non-sterile soil. The results showed that inoculation with bacterial treatments had a more stimulating effect on growth and development of plants in nonsterile than sterile soil. In the third experiment, Inoculation of maize seeds with all bacterial strains significantly increased plant height, 100 seed weight, number of seed per ear and leaf area. The results also showed significant increase in ear and shoot dry weight of maize.

  17. Halotolerant/alkalophilic bacteria associated with the cyanobacterium Arthrospira platensis (Nordstedt Gomont that promote early growth in Sorghum bicolor (L. Moench

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    Gómez G. Liliana Cecilia

    2012-04-01

    Full Text Available

    Arthrospira platensis associated bacteria (APAB identified through molecuar biology like Bacillus okhensis, Indibacter alkaliphilus and Halomonas sp., are also producing 3-indol acetic acid (IAA, these bacteria was used in early plant growth promotion tests over Sorghum bicolor, these bioassay was considered indirect evidence to suggest that APAB also may have stimulatory effects over A. platensis growth naturally. I. alkaliphilus and B. okhensis enhanced early germination of S. bicolor seads, with better results than that achieved by Azospirillum brasilense, bacterium used like reference as a common plant growth promoting rizobacteria. The three APAB enhanced significative differences (P≤0.05 over morphoagronomic parameters, I. alkaliphilus and B. okhensis exhibit better resoults in elongation stimulation and root and foliage dry weight. Above evidence suggest this bacteria like plant growth promoting and it recomended testing with A. platensis axenic cultures and its associated bactteri for understanding true interaction between them.

  18. Compatible bacterial mixture, tolerant to desiccation, improves maize plant growth.

    Directory of Open Access Journals (Sweden)

    Dalia Molina-Romero

    Full Text Available Plant growth-promoting rhizobacteria (PGPR increase plant growth and crop productivity. The inoculation of plants with a bacterial mixture (consortium apparently provides greater benefits to plant growth than inoculation with a single bacterial strain. In the present work, a bacterial consortium was formulated containing four compatible and desiccation-tolerant strains with potential as PGPR. The formulation had one moderately (Pseudomonas putida KT2440 and three highly desiccation-tolerant (Sphingomonas sp. OF178, Azospirillum brasilense Sp7 and Acinetobacter sp. EMM02 strains. The four bacterial strains were able to adhere to seeds and colonize the rhizosphere of plants when applied in both mono-inoculation and multi-inoculation treatments, showing that they can also coexist without antagonistic effects in association with plants. The effects of the bacterial consortium on the growth of blue maize were evaluated. Seeds inoculated with either individual bacterial strains or the bacterial consortium were subjected to two experimental conditions before sowing: normal hydration or desiccation. In general, inoculation with the bacterial consortium increased the shoot and root dry weight, plant height and plant diameter compared to the non-inoculated control or mono-inoculation treatments. The bacterial consortium formulated in this work had greater benefits for blue maize plants even when the inoculated seeds underwent desiccation stress before germination, making this formulation attractive for future field applications.

  19. Compatible bacterial mixture, tolerant to desiccation, improves maize plant growth.

    Science.gov (United States)

    Molina-Romero, Dalia; Baez, Antonino; Quintero-Hernández, Verónica; Castañeda-Lucio, Miguel; Fuentes-Ramírez, Luis Ernesto; Bustillos-Cristales, María Del Rocío; Rodríguez-Andrade, Osvaldo; Morales-García, Yolanda Elizabeth; Munive, Antonio; Muñoz-Rojas, Jesús

    2017-01-01

    Plant growth-promoting rhizobacteria (PGPR) increase plant growth and crop productivity. The inoculation of plants with a bacterial mixture (consortium) apparently provides greater benefits to plant growth than inoculation with a single bacterial strain. In the present work, a bacterial consortium was formulated containing four compatible and desiccation-tolerant strains with potential as PGPR. The formulation had one moderately (Pseudomonas putida KT2440) and three highly desiccation-tolerant (Sphingomonas sp. OF178, Azospirillum brasilense Sp7 and Acinetobacter sp. EMM02) strains. The four bacterial strains were able to adhere to seeds and colonize the rhizosphere of plants when applied in both mono-inoculation and multi-inoculation treatments, showing that they can also coexist without antagonistic effects in association with plants. The effects of the bacterial consortium on the growth of blue maize were evaluated. Seeds inoculated with either individual bacterial strains or the bacterial consortium were subjected to two experimental conditions before sowing: normal hydration or desiccation. In general, inoculation with the bacterial consortium increased the shoot and root dry weight, plant height and plant diameter compared to the non-inoculated control or mono-inoculation treatments. The bacterial consortium formulated in this work had greater benefits for blue maize plants even when the inoculated seeds underwent desiccation stress before germination, making this formulation attractive for future field applications.

  20. Effect of plant growth-promoting bacteria on the growth and fructan production of Agave americana L.

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    Neyser De La Torre-Ruiz

    Full Text Available ABSTRACT The effect of plant growth-promoting bacteria inoculation on plant growth and the sugar content in Agave americana was assessed. The bacterial strains ACO-34A, ACO-40, and ACO-140, isolated from the A. americana rhizosphere, were selected for this study to evaluate their phenotypic and genotypic characteristics. The three bacterial strains were evaluated via plant inoculation assays, and Azospirillum brasilense Cd served as a control strain. Phylogenetic analysis based on the 16S rRNA gene showed that strains ACO-34A, ACO-40 and ACO-140 were Rhizobium daejeonense, Acinetobacter calcoaceticus and Pseudomonas mosselii, respectively. All of the strains were able to synthesize indole-3-acetic acid (IAA, solubilize phosphate, and had nitrogenase activity. Inoculation using the plant growth-promoting bacteria strains had a significant effect (p < 0.05 on plant growth and the sugar content of A. americana, showing that these native plant growth-promoting bacteria are a practical, simple, and efficient alternative to promote the growth of agave plants with proper biological characteristics for agroindustrial and biotechnological use and to increase the sugar content in this agave species.

  1. Microbial Inoculation Improves Growth of Oil Palm Plants (Elaeis guineensis Jacq.).

    Science.gov (United States)

    Om, Azlin Che; Ghazali, Amir Hamzah Ahmad; Keng, Chan Lai; Ishak, Zamzuri

    2009-12-01

    Introduction of diazotrophic rhizobacteria to oil palm tissues during the in vitro micropropagation process establishes an early associative interaction between the plant cells and bacteria. In the association, the diazotrophs provide the host plants with phytohormones and fixed nitrogen. This study was conducted to observe growth of bacterised tissue cultured oil palm plants under ex vitro conditions after 280 days of growth. Root dry weight, shoot dry weight, root volume, bacterial colonisation, leaf protein and chlorophyll content of the host plants were observed. The results revealed that the inocula successfully colonised roots of the host plants. Plants inoculated with Acetobacter diazotrophicus (R12) had more root dry weight and volume than plants inoculated with Azospirillum brasilense (Sp7). Leaf protein and chlorophyll content were higher in the bacterised plants compared to Control 2 plants (inoculated with killed Sp7). These results suggest that the diazotrophs successfully improved the growth of the host plant (oil palm) and minimised the amount of N fertiliser necessary for growth.

  2. Evaluación de la asociación bacterias fijadoras de nitrógeno - líneas interespecíficas de arroz-nitrógeno, en Typic haplustalf. Ibagué, Colombia

    Directory of Open Access Journals (Sweden)

    Margarita M Vallejo

    2008-01-01

    Full Text Available El estudio se llevó a cabo en la hacienda Cauchitos, municipio de Ibagué, departamento del Tolima (Norte 4° 23' 51" y Oeste 75° 9' 7", 979 msnm, 24.3°C, bosque seco tropical (bs-T, con el objetivo de evaluar las asociaciones entre bacterias fijadoras de nitrógeno con inóculo y sin él en diez líneas interespecíficas de arroz, con tres dosis de nitrógeno (0%, 50% y 100% de 250 kg/ha-1 y tres repeticiones por tratamiento. La inoculación se realizó con 1 cm³ de unidades formadoras de colonias por 250 g de semilla de cada cultivar. Se aislaron 2.260 bacterias de los géneros Azotobacter spp y Azospirillum spp, se identificaron las especies A. brasilense, A. lipoferum, A. amazonense y del género Azotobacter las especies A. chroococcum, A. vinelandii, A. paspali y A. beijerinckii. Respecto al inóculo no se encontraron diferencias significativas al realizar su aplicación, se determinó que Azotobacter spp y Azospirillum spp fueron géneros típicos de la flora bacteriana en el cultivo del arroz y en condiciones de campo hubo efecto de los tratamientos en la flora bacteriana, y Azotobacter spp fue el predominante en cada uno de los tratamientos.

  3. Chlorella sorokiniana UTEX 2805, a heat and intense, sunlight-tolerant microalga with potential for removing ammonium from wastewater.

    Science.gov (United States)

    de-Bashan, Luz E; Trejo, Adan; Huss, Volker A R; Hernandez, Juan-Pablo; Bashan, Yoav

    2008-07-01

    In the summer of 2003, a microalga strain was isolated from a massive green microalgae bloom in wastewater stabilization ponds at the treatment facility of La Paz, B.C.S., Mexico. Prevailing environmental conditions were air temperatures over 40 degrees C, water temperature of 37 degrees C, and insolation of up to 2400 micromol m2 s(-1) at midday for several hours at the water surface for four months. The microalga was identified as Chlorella sorokiniana Shih. et Krauss, based on sequencing its entire 18S rRNA gene. In a controlled photo-bioreactor, this strain can grow to high population densities in synthetic wastewater at temperatures of 40-42 degrees C and light intensity of 2500 micromol m2 s(-1) for 5h daily and efficiently remove ammonium from the wastewater under these conditions better than under normal lower temperature (28 degrees C) and lower light intensity (60 micromol m2 s(-1)). When co-immobilized with the bacterium Azospirillum brasilense that promotes growth of microalgae, the population of microalga grew faster and removed even more ammonium. Under exposure to extreme growth conditions, the quantity of four photosynthetic pigments increased in the co-immobilized cultures. This strain of microalga has potential as a wastewater treatment agent under extreme conditions of temperature and light intensity.

  4. The crude plant juices of desert plants as appropriate culture media for the cultivation of rhizospheric microorganisms

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    Eman H. Nour

    2012-01-01

    Full Text Available The exclusive use of plant juices, not as a mere supplement to synthetic culture media, for culturing rhizospheric microorganisms (RMO is introduced here. Juices were prepared from desert (Mesembryanthemum crystallinum L., Zygophyllum album L., Carpobrotus edulis L. as well as cultivated (Trifolium alexandrinum L., Beta vulgaris L. plants. Colonies of RMO (Azospirillum brasilense, Enterobacter agglomerans and Klebsiella pneumoniae nicely developed on surface-inoculated agar plates prepared from crude and diluted juice of M. crystallinum (ice plant. Furthermore, hundreds of RMO colonies developed on various standard culture media were replicated (>90% on agar plates of different plant juices. RMO cells grew nicely in liquid ice plant juice, with doubling times comparable to those grown in the reference culture medium. RMO populations resident in various host plants were able to develop on culture media prepared from homologous and heterologous juices. The application of a thin semi-solid overlay agar on the surfaces of inoculated agar plates significantly increased the recovery of micro-colonies on agar plates, particularly those prepared from plant juices.

  5. Die Korrespondenz zwischen Petar Konjovic (1883-1970 und Zdenek Chalabala (1899-1962

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    Mosusova Nadežda

    2002-01-01

    Full Text Available (nemački Slawische Gemeinsamkeit und auch politische Ursachen brachten nach dem Ersten Weltkriege zwei neue Staaten, zusammen, Jugoslawien und Tschechoslowakei. Kulturelle Beziehungen waren sehr lebendig (sie waren auch aktuell vor dem Kriege auf dem musikalischen Gebiete, in dem Konzertsaal, in der Oper. Besondere Aufmerksamkeit erhob die Oper Koštana (1929 von Petar Konjovic, mit der Premiere in damaligen Jugoslawien in 1931 (Zagreb, Belgrad Ljubljana, die sie auch nach Tschechoslowakei brachte. Nach der Aufführung in dem Nationaltheater Brno/Brünn (ins Tschechisch übersetzt von Zdenek Knittl, September 1932, für deren Erfolg sich der tschechische Dirigent Zdenek Chalabala sehr engagiert hatte, kam die Oper Koštana in die Sphäre der künstlerischen Aufmerksamkeit des Westens (z. B. Universal war sehr interessiert und dabei auf die Szene des Prager Nationaltheater 1935, auch unter der Leitung von Chalabala. Seine Anstrengungen waren keine blosse Höflichkeit, überbieten jedenfalls eine normale zwischenstaatliche Kurtoasie und auch beide Nationaltheater, nich ohne Verdienst dieses Dirigenten stellten ihre beste Besetzungen dem serbischen Tonsätzer zur Verfügung. Leider verhinderte der Zweite Weltkrieg Koštanas "Drang nach Westen". In der Nachkriegszeit erlebte diese Oper eine Premiere in Bratislava (Slowakei 1948, und noch ein Gastspiel der Belgrader Oper in Warschau (l960. In der Korrespondenz Konjovic - Chalabala handelt sich um die Vorbereitungen der beiden tschechischen Premieren und um die Bearbeitung der Koštana, von Chalabala initiert und von dem Komponisten durchgeführt oder autorisiert. Der Briefumtausch dauerte 1932-1940, mit cca 100 Schreiben (hier nur ein Viertel geschildert. Ein Teil der Korrespondenz, die Briefe von Konjovic zu Chalabala, kamen auf private Weise von der Dirigenten Witwe Bela Rosumova (Opernsängerin, Brünner Koštana durch die damalige pensionierte Solistin der Zagreber Oper, Marta Wellar-Rak (

  6. Das Problem der sogenannten zusammengesetzten Bezirke auf dem Balkan im 11. Jahrhundert: Zwei fallbeispiele

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    Krsmanović Bojana

    2009-01-01

    Full Text Available (nemački In der vorliegenden Arbeit wurden Charakteristika der sogenannten zusammengesetzten militärisch-zivilen Bezirke behandelt, die aus zwei oder drei Untereinheiten bestanden. Diese Untereinheiten der zusammengesetzten Bezirke hatten in der Regel feste zivile und militärische Verwaltungsstrukturen, d.h. sie hatten eine gewisse Unabhängigkeit. Ein zusammengesetzter Bezirk konnte in den Quellen als ein 'thema' (im Singular bezeichnet werden oder auch im Plural als 'themata'. An seiner Spitze befand sich ein Dux/katepano oder gelegentlich auch ein Stratege. Der zivilen Verwaltung stand ein Richter/Prätor vor, welcher häufig das Amt eines anagrapheus innehatte. Weiterhin wurde der zusammengesetzte Bezirk Voleron-Strymon-Thessalonike naher untersucht sowie die Probleme, die mit dem Status seiner Unterheiten, insbesondere mit Voleron, zusammenhängen. Es ist möglich, dass aus denjenigen Gebieten des Balkans, die nach dem Jahr 1018 unter byzantinische Herrschaft gefallen waren, ein zusammengesetzter Bezirk gebildet wurde. Die Rede ist von dem Thema Bulgaria-Sirmium-Paradounavon. Es wird vermutet, dass die demographischen Umstände sowie die ethnische Zusammensetzung der Bevölkerung der Region Einfluss auf die Charakteristika des zusammengesetzten Bezirks hatten. Die zivile Verwaltungsstruktur war hier nicht derart beständig wie in den anderen Gebieten des Balkans, die bereits vor dem Krieg von 976-1018 unter byzantinischer Herrschaft standen. Sirmium und Paradounavon scheinen im zivilen Bereich der Verwaltung von Amtsträgern aus Bulgarien geleitet worden zu sein, wahrend die militärische Führung anscheinend unabhängig von Bulgarien war.

  7. Privatiserung von Geschichte. Probleme einer differenzierten Aufarbeitung History of war as a private affair

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    Birthe Kundrus

    2000-11-01

    Full Text Available Ausgangspunkt der Untersuchung von Vera Neumann ist die These, daß im Krieg Erlebtes und Erlittenes in den Wiederaufbau- und Wirtschaftswunderjahren Westdeutschlands einem Thematisierungstabu unterlegen sei. Anhand von 50 Interviews, die in den 80er Jahren im Rahmen des von Lutz Niethammer geleiteten Projekts „Lebensgeschichte und Sozialgeschichte im Ruhrgebiet 1930–1960“ (LUSIR entstanden sind und die sie jetzt anhand der Tonbandprotokolle und Abschriften ein zweites Mal auswertet, möchte die Historikerin diese Verschüttungen aufspüren. Im Anschluß an Niethammers These der „Privatisierung von Geschichte“ will sie zeigen, daß seelische und körperliche Kriegsfolgen wie Deprivations- und Überlastungsgefühle, der Verlust von Angehörigen und Kriegsbeschädigungen „privatisiert“, d.h. an die Familien übertragen wurden. Dort seien dann in erster Linie die weiblichen Familienmitglieder mit der Versorgungs- und Pflegearbeit konfrontiert worden. An vier Fallbeispiele schließt sich eine detaillierte Auswertung des gesamten Interviewmaterials an. Diesen Teilen folgt ein Abschnitt zur staatlichen Kriegsopferversorgung in der frühen Bundesrepublik.Vera Neumann’s ‘book examines the question, how German women and men dealt with traumatic experiences of World War II in the years of the “German Miracle”. It argues that the emotional and financial strainth due to the war were “privatized” in the families, where especially the women had to master the various problems. Its main sources are interviews, which were made during the eighties by the LUSIR-project.

  8. Evaluation of potential crushed-salt constitutive models

    Energy Technology Data Exchange (ETDEWEB)

    Callahan, G.D.; Loken, M.C.; Sambeek, L.L. Van; Chen, R.; Pfeifle, T.W.; Nieland, J.D. [RE/SPEC Inc., Rapid City, SD (United States); Hansen, F.D. [Sandia National Labs., Albuquerque, NM (United States). Repository Isolation Systems Dept.

    1995-12-01

    Constitutive models describing the deformation of crushed salt are presented in this report. Ten constitutive models with potential to describe the phenomenological and micromechanical processes for crushed salt were selected from a literature search. Three of these ten constitutive models, termed Sjaardema-Krieg, Zeuch, and Spiers models, were adopted as candidate constitutive models. The candidate constitutive models were generalized in a consistent manner to three-dimensional states of stress and modified to include the effects of temperature, grain size, and moisture content. A database including hydrostatic consolidation and shear consolidation tests conducted on Waste Isolation Pilot Plant and southeastern New Mexico salt was used to determine material parameters for the candidate constitutive models. Nonlinear least-squares model fitting to data from the hydrostatic consolidation tests, the shear consolidation tests, and a combination of the shear and hydrostatic tests produces three sets of material parameter values for the candidate models. The change in material parameter values from test group to test group indicates the empirical nature of the models. To evaluate the predictive capability of the candidate models, each parameter value set was used to predict each of the tests in the database. Based on the fitting statistics and the ability of the models to predict the test data, the Spiers model appeared to perform slightly better than the other two candidate models. The work reported here is a first-of-its kind evaluation of constitutive models for reconsolidation of crushed salt. Questions remain to be answered. Deficiencies in models and databases are identified and recommendations for future work are made. 85 refs.

  9. Das Internet als Medium für Demokratisierung und Verständnisbildung - Beispiel Bosnien-Herzegowina

    Directory of Open Access Journals (Sweden)

    Michael Reimann

    2002-10-01

    Full Text Available "Politik und Internet" ist ein weites Feld für praktische Anwendungsversuche wie für wissenschaftliche Begleitforschung geworden. Der Artikel untersucht, inwieweit Informations- und Kommunikationstechnologien (IKT im allgemeinen und das "World Wide Web" im besonderen in einem vom Krieg zerrissenen Land wie Bosnien-Herzegowina im Sinne von "peacebuilding" für Demokratisierung und Verständigung zwischen den (ehemaligen Konfliktparteien eingesetzt werden können. Aus Ansätzen der Friedens- und Konfliktforschung, sowie der Informationswissenschaft werden dazu Kriterien abgeleitet, die für einen adäquaten Einsatz des Internets erfüllt sein sollten, bzw. aus denen abgeleitet werden kann, wie Internetangebote gestaltet sein sollten, damit sie entsprechend wirken können. Im empirischen Teil wird zunächst die Problematik des "Allgemeinen Zugangs" zum Internet in Bosnien-Herzegowina dargestellt, indem Daten zur Telekommunikationsinfrastruktur und zur Verbreitung und Nutzung des Internets gegeben und Projekte zur Verbesserung der Situation vorgestellt werden. Im weiteren werden die entwickelten Kriterien an ausgewählte IKT-Projekte und Internet-Homepages nationaler und internationaler Institutionen und Organisationen angelegt, die an Demokratisierung und Verständnisbildung beteiligt sind. Als Beispiel für "bad practice" wird die Homepage des "Office of the High Representative" der Vereinten Nationen dargestellt, da diese Website fast ausschliesslich in englischer Sprache gehalten und somit für bosnische Bürger nahezu unzugänglich ist. Als Beispiele für "good practice" - zumindest in bezug auf bestimmte Kriterien - können die Homepages der bosnischen Zentralbank, der "Mine Action Centers", sowie der OSZE Mission in Bosnien-Herzegowina gelten. Das im Bereich Bildung angesiedelte Projekt "D@dalos" deckt so gut wie alle entwickelten Kriterien ab; es ist konfliktlösungsorientiert und kann somit als ein echtes "Demokratisierungsprojekt" gelten

  10. Griechische Mythologie bei Statius : Dante, Harold Bloom und die Grenzen der politischen Psychologie

    Directory of Open Access Journals (Sweden)

    Marko Marinčič

    2010-07-01

    Full Text Available Ausgangspunkt des Aufsatzes ist die Biographie des Statius, wie sie Dante in der Göttlichen Komödie(Purg. 22 dem Autor der Thebais, der Siluae und des Achilleis-Fragments in den Mund legt. Die Geschichte von der heimlichen Bekehrung des Statius unter Domitian, die die Mehrzahl der modernen Interpreten als eine literarische Fiktion Dantes betrachtet, stützt sich in Purg. 22.88–91 auf eine psychologische Deutung der Thebais, derzufolge Statius’ Schilderung des thebanischen Bürgerkriegs als ein Symptom der Glaubensverleugnung aufzufassen sei. Es kann angenommen werden, daß die kreative Erfindung der religiösen Krise des Statius auf einer politisch-psychologischen Betrachtung der flavischen Literatur gründet, die Dante mit einer großen Zahl moderner Interpreten teilt. Die Tatsache, daß Statius einen mythischen Krieg als Sujet für sein Epos wählt, und die allgemeine Vorliebe der flavischen Epik für mythologische Stoffe werden noch heute oft auf zwei komplementär zusammenwirkende Faktoren zurückgefürt: auf die politische Unterdrückung, die eine affirmative Beschäftigung mit Zeitgschichte schwierig macht, und – in Sinne von Harold Blooms anxiety of influence – auf die künstlerische Beengung, die die nachvergilischen Epiker als Nachfolger eines kanonisierten literarischen ‘Vaters’ erfahren. Der Autor des Beitrags betont einerseits die künstlerische Expressivität, die Dantes ‘Psychopathogramm der nachaugusteischen Epik’ kennzeichnet, und untersucht andererseits – am Beispiel der Achilleis – die Grenzen der politischen Psychologie im Bereich der Literaturgeschichte.

  11. Sexuelle Gewalt in Besatzungsalltag und Vernichtungskrieg Sexual Violence in Daily Life of Occupation and War of Extermination

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    Silke Schneider

    2005-03-01

    Full Text Available Als Grundlage für ihre Analyse des Umgangs der Wehrmacht mit sexueller Gewalt im Zweiten Weltkrieg dienen Birgit Beck die Akten militärgerichtlicher Verfahren. Das Thema wird von der Autorin in die Geschichte der sexuellen Gewalt in Kriegen von der Frühen Neuzeit bis zur Gegenwart eingeordnet. Ziel ist die Überprüfung geschichts- und sozialwissenschaftlicher Annahmen über die Ursachen sexueller Gewalt im Krieg am konkreten historischen Beispiel. Die Gründe für die militärgerichtliche Verfolgung sexueller Gewalt – die nur einen Bruchteil der Verfahren insgesamt darstellt – sieht Beck in erster Linie in übergeordneten militärischen Verhaltensvorgaben, dem Männerbild der Wehrmacht und dem Schutz der deutschen „Volksgemeinschaft“ begründet; für einen systematischen Einsatz sexueller Gewalt durch die Wehrmacht bieten die untersuchten Verfahren keine ausreichenden Belege.Birgit Beck uses the files of military court actions as the foundation for her analysis of the way in which the German armed forces dealt with sexual violence during the Second World War. She locates the topic on the timeline of the history of sexual violence in war from the early modern era to the present. The goal is the review of historical and social scientific assumptions about the causes of sexual violence in war. The author sees the reasons for the military court’s persecution of sexual violence—which only presents a miniscule portion of the entire proceedings—first and foremost in the extremely ordered military behavioral guidelines, which account for both the male image of the armed forces and the protection of the German “community of folk.” Clues as to a systematic deployment of sexual violence by the German armed forces cannot, according to Beck, be derived from the proceedings.

  12. Rise and crisis of the German power companies. At the same time a contribution on the development of the energy law; Aufstieg und Krise der deutschen Stromkonzerne. Zugleich ein Beitrag zur Entwicklung des Energierechts

    Energy Technology Data Exchange (ETDEWEB)

    Becker, Peter

    2010-07-01

    The regulation of network use rates has reached a decisive stage. The German federal and states authorities have passed most of the electricity rates regulations and some of the gas rate regulations. The problem is now whether they will hold in court proceedings. The contribution attempts to clarify the picture by presenting a historical outline of power grid regulation. (orig./GL) [German] Der Aufstieg der Stromkonzerne beginnt mit drei genialen Unternehmern: Werner Siemens, Erfinder des Dynamos, Walter Rathenau, Gruender der AEG, und Hugo Stinnes, Zechenerbe, der mit einem genialen Coup das Rheinisch-Westfaelische Elektrizitaetswerk, RWE, gross machte: Wirtschaftsgeschichte, spannend und farbig erzaehlt. Die Konzerne waren so maechtig, dass sie bis ins Dritte Reich hinein das Entstehen rechtlicher Regeln verhindern konnten, die sie bei ihren Kartellabsprachen nur behindert haetten. Die Erklaerung ist einfach: Sie gehoerten, wie auch die Stadtwerke, weit ueberwiegend dem Staat, der natuerlich kein Interesse hatte, seine Wirtschaftstaetigkeit einzuengen. Erst die Nazis wollten mit ihrem Energiewirtschaftsgesetz von 1935 Preise regulieren; fuer die Aufruestung. Nach dem Krieg entstand unter dem Druck der Alliierten ein Kartellgesetz - aber nicht fuer die Energiewirtschaft. Erst die Liberalisierung - auf Druck aus Bruessel - schob die Gesetzesarbeit an: das Kartellgesetz, das Energiewirtschaftsgesetz, im Jahr 2005 dann zahlreiche Verordnungen. Dem Staat gefaellt die Regelungswut, die Komplexitaet nimmt zu... Aber das Buch erzaehlt die Entwicklung anhand zahlreicher konkreter Streitfaelle, differenziert, trotzdem uebersichtlich - und immer spannend. Einen derartigen Ueberblick gab es bisher nicht. Dabei ist er ueberfaellig, weil sich der Klimaschutz nicht nur auf den Aufstieg der Erneuerbaren Energien auswirkt, mit dem der Abstieg der Stromkonzerne einher geht. Die rechtlichen Regeln betreffen immer mehr Menschen: Verbraucher, Windkraft- und Photovoltaikbetreiber, die

  13. CARACTERIZACIÓN DE BACTERIAS DIAZOTRÓFICAS ASIMBIÓTICAS ASOCIADAS AL EUCALIPTO (Eucalyptussp. EN CODAZZI, CESAR (COLOMBIA Characterization of Diazotrophic Bacteria Non-Symbiotic Associated with Eucalyptus (Eucalyptussp. in Codazzi, Cesar (Colombia

    Directory of Open Access Journals (Sweden)

    DOLLY MELISSA OBANDO CASTELLANOS

    Full Text Available Se evaluó el efecto de las épocas climáticas (lluvia y sequía y del estrato de la muestra (suelo rizosférico, raíces y hojas sobre la población de los géneros Azotobacter, Beijerinckia, Derxia, Azospirillum, Herbaspirillum, Gluconacetobacter y Burkholderia en el Eucalipto (Eucalyptus sp.. Así mismo, se evalúo su capacidad en la producción de compuestos indólicos como promotores del crecimiento vegetal y su actividad de reducción de acetileno como indicador de la fijación biológica de nitrógeno. Los resultados no registraron diferencias estadísticas significativas en el test de Tukey (P ≤ 0.05 en la población con respecto a la época climática. Con respecto al estrato de muestra, los aislamientos tentativos de Herbaspirillum sp. y Azospirillum sp. presentaron diferencias significativas en suelo rizosférico y raíces. Se obtuvieron 44 aislamientos de los cuales se agruparon por caracterización fenotípica como: 14 presuntivos de Beijerinckia sp., 12 de Azotobacter sp., ocho de Derxia sp., cuatro de Herbarpirillum sp., cinco de Azospirillum sp., uno de Gluconacetobacter sp. y uno de Burkholderia sp. Por su alto potencial fueron seleccionados y criopreservados los aislamientos C27, C26 y C25, las cuales presentaron los mejores valores de eficiencia in vitro, superando valores de producción de las cepas de referencia utilizadas (A. chroococcum (AC1 y A. brasilense (SP7.The effect of climatic seasons (rainy and dry and the stratum sample (rhizospheric soil, roots and leaves the population of the genera Azotobacter, Beijerinckia, Derxia, Azospirillum, Herbaspirillum, Gluconacetobacter and Burkholderia in soil rhizosphere, roots and leaves of Eucalyptus (Eucalyptus sp.. It also assesses their ability to produce indoles compounds as plant growth promoters and their acetylene reduction activity as an indicator of biological fixation of nitrogen. The results showed no statistically significant differences in the Duncan test (P ≤ 0

  14. Evaluation of the association nitrogen fixing bacterias interspecific – rice lines – nitrogen, in typic haplustalf. Ibagué, Colombia Evaluación de la asociación bacterias fijadoras de nitrógeno – líneas interespecíficas de arroz–nitrógeno, en Typic haplustalf. Ibagué, Colombia

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    Bonilla Correa Carmen Rosa

    2008-03-01

    Full Text Available The study was carried out at the Cauchitos farm, Ibague municipality department of Tolima, with bounds: North 4°23'51"; and west 75°9'7";, 979 ansm, the average temperature is 24,3°C, tropical dry forest (bs–t in the Holdridge classification. The purpose of this study was to evaluate the association between the nitrogen fixation bacteries with and without inoculo in 10 interespecific rice lines with three nitrogen dosis (0, 50 and 100% de 250 kg/ ha–1 and three repetitions. The inoculation was realized with 108 former units of colonies per millimeter. 2.260 bacteries of the generums Azotobacter spp y Azospirrillum spp., and identification the species: Azospirillum brasilense, Azospirillum lipoferum, Azospirillum amazonense, were identified and from the genus Azotobacter were identified the species: A. choroococcum, A. vinelandii, A. paspali and A. veijerinckii. Weren't found none significative differences after the inoculation. Azotobacter spp and Azospirillum spp. were typical generums of the bacterian flora in the rice plantation and in field conditions were effect of the treatment effects in the bacterian . The Azotobacter spp was the predominant in generum in each one of the treatments.El estudio se llevó a cabo en la hacienda Cauchitos, municipio de Ibagué, departamento del Tolima (Norte 4° 23' 51"; y Oeste 75° 9' 7";, 979 msnm, 24.3°C, bosque seco tropical (bs–T, con el objetivo de evaluar las asociaciones entre bacterias fijadoras de nitrógeno con inóculo y sin él en diez líneas interespecíficas de arroz, con tres dosis de nitrógeno (0%, 50% y 100% de 250 kg/ha–1 y tres repeticiones por tratamiento. La inoculación se

  15. Ocorrência de bactérias diazotróficas em diferentes genótipos de cana-de-açúcar Occurrence of diazotrophic bacteria in different sugar cane genotypes

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    FÁBIO BUENO DOS REIS JUNIOR

    2000-05-01

    Full Text Available O objetivo deste trabalho foi avaliar a localização e o número de bactérias endofíticas em quatro genótipos de cana-de-açúcar e investigar sobre a possível existência de correlação com os resultados apresentados em trabalhos de quantificação da fixação biológica de nitrogênio (FBN. Fez-se um levantamento das bactérias diazotróficas presentes, e quantificou-se a população de Herbaspirillum spp. e Acetobacter diazotrophicus, em genótipos de cana-de-açúcar contrastantes quanto à capacidade de obter N da FBN. De acordo com o levantamento realizado neste trabalho, as bactérias estudadas (Azospirillum lipoferum, A. brasilense, A. amazonense, Herbaspirillum spp. e Acetobacter diazotrophicus estavam presentes nos quatro genótipos avaliados e em todas as partes da planta, exceto A. amazonense, que não foi isolado de amostras de folhas. A quantificação das bactérias Herbaspirillum spp. e A. diazotrophicus mostrou não haver diferenças significativas entre os genótipos, e que, geralmente, elas estão presentes em maior número nas raízes. Enquanto Herbaspirillum spp. mantém-se mais estável ao longo do ciclo da cultura, a população de A. diazotrophicus decresce com a aproximação do final do ciclo comercial. Pode-se sugerir que as diferenças entre as taxas de FBN encontradas nos diversos genótipos não é causada por diferenças na presença ou no número das bactérias aqui estudadas.The objective of this work was to find out the localization and number of endophytic bacteria in four sugar cane genotypes and investigate upon the possible existence of correlation to the results obtained in some studies about quantification of biological nitrogen fixation (BNF. A survey of the diazotrophic bacteria present in sugar cane genotypes differing in their capacity to obtain nitrogen through BNF was performed, and population of Herbaspirillum spp. and Acetobacter diazotrophicus was quantified. The bacteria tested in the

  16. COMO FAZER UMA AGRICULTURA VERDE USANDO O MAIS ANTIGO PROCESSO DE OBTENÇÃO DE NITROGÊNIO EM PLANTAS

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    Veronica Massena Reis

    2013-06-01

    Full Text Available Around 78% of the air is composed by nitrogen and this element is the third most important to the maintenance of life on the planet, after the carbon (C and oxygen (O. The greatparadox is life in earth it that this gas (N2 is inert and can only be reduced to a form assimilated by plants by different genus of bacteria called diazotrophs. These bacteria interact with other organisms including agricultural crops as the most famous one, the rhizobia of soybean. Another example recently incorporated to the cereal production routine is the application of another bacterium called Azospirillum brasilense described by the research group of Johanna Döberenier in the 80’s. The association of diazotrophs and grasses has been studied for about 60 years and revealed that in addition to providing nitrogen to the crops (contributing with up to 58% of its necessity, can also produce phyto-hormones, act as a biocontrol agents, solubilize phosphates and some other features; helping plants in the response of environmental stresses and refl ecting in crop productivity using reduced doses of N-fertilizer. With the objective of develop a cropmanagement based on biological nitrogen fi xation and a recommendation of inoculant application in order to improve the utilization of N-fertilizers, also preconized by the green revolution, made this research recognized worldwide for its innovation that advocates the use of a conservative practice, keeping the man in the fi eld, producing more without harming the environment.

  17. Restoration of eroded soil in the Sonoran Desert with native leguminous trees using plant growth-promoting microorganisms and limited amounts of compost and water.

    Science.gov (United States)

    Bashan, Yoav; Salazar, Bernardo G; Moreno, Manuel; Lopez, Blanca R; Linderman, Robert G

    2012-07-15

    Restoration of highly eroded desert land was attempted in the southern Sonoran Desert that had lost its natural capacity for self-revegetation. In six field experiments, the fields were planted with three native leguminous trees: mesquite amargo Prosopis articulata, and yellow and blue palo verde Parkinsonia microphylla and Parkinsonia florida. Restoration included inoculation with two of plant growth-promoting bacteria (PGPB; Azospirillum brasilense and Bacillus pumilus), native arbuscular mycorrhizal (AM) fungi, and small quantities of compost. Irrigation was applied, when necessary, to reach a rainy year (300 mm) of the area. The plots were maintained for 61 months. Survival of the trees was marginally affected by all supplements after 30 months, in the range of 60-90%. This variation depended on the plant species, where all young trees were established after 3 months. Plant density was a crucial variable and, in general, low plant density enhanced survival. High planting density was detrimental. Survival significantly declined in trees 61 months after planting. No general response of the trees to plant growth-promoting microorganisms and compost was found. Mesquite amargo and yellow palo verde responded well (height, number of branches, and diameter of the main stem) to inoculation with PGPB, AM fungi, and compost supplementation after three months of application. Fewer positive effects were recorded after 30 months. Blue palo verde did not respond to most treatments and had the lowest survival. Specific plant growth parameters were affected to varying degrees to inoculations or amendments, primarily depending on the tree species. Some combinations of tree/inoculant/amendment resulted in small negative effects or no response when measured after extended periods of time. Using native leguminous trees, this study demonstrated that restoration of severely eroded desert lands was possible. Copyright © 2012 Elsevier Ltd. All rights reserved.

  18. The Effect of Plant Growth Promoting Rhizobacteria (PGPR and Phosphate Solubilizing Microorganism (PSM on Yield and Yield Components of Wheat (cv. N80 under Different Nitrogen and Phosphorous Fertilizers Levels in Greenhouse Condition

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    S. H Bahari saravi

    2013-04-01

    Full Text Available In order to evaluate the effect of plant growth promoting rhizobacteria (PGPR and phosphate solubilizing microorganism (PSM on yield and yield components of wheat a pot experiment was conducted at Sari Agricultural Sciences and Natural Resources University during 2009. Experiment was arranged in factorial based on completely randomized design in three replicates. Treatments were included bio-fertilizer in four levels (non-inoculation control, Phosphate Barvare 2 (Pseudomonas fluorescens+Bacillus subtilis, Supernitroplus (Azotobacter brasilense+Azospirillum lipoferum and Nitroxine (Azospirillum + Pseudomona + Bacillus, three levels of chemical nitrogen fertilizer (0, 75 and 150 kg urea/ha and three levels of phosphorus fertilizer (0, 60 and 120 kg super phosphate triple/ha. Results showed that the studied treatments (biofertilizer, nitrogen and phosphate inorganic fertilizers had significant effect on grain number per spike, 1000 grain weight, grain yield, straw yield, biological yield and harvest index. Interaction effect between biofertilizer and chemical fertilizers was significant in terms of grain yield. The maximum grain yield was resulted from simultaneously applying of Nitroxine and 75 kg ha-1 nitrogen fertilizer. By contrast, the highest straw yield was obtained when 150 kg nitrogen fertilizer was used. Grain yield had the maximum correlation with biological yield (r=0.85**. Grain yield positively and significantly correlated with grain number per spike (r=0.73**, 1000 grain weight (r=0.68**, straw yield (r=0.56** and harvest index (r=0.69**. In conclusion biofertilizer inoculations could reduce application of nitrogen and phosphorus chemical fertilizers and increase plant performance.

  19. Effect of plant growth promoting rhizobacteria (PGPR application, nitrogen and zinc sulphate fertilizer on yield and nitrogen uptake in rapeseed (Brassica napus L.

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    N. Jafari

    2016-05-01

    Full Text Available In order to study the effects of simultaneous application of ZnSO4 and biological fertilizer, Azotobacter chroococcum and Azospirillum brasilense, on grain yield and nitrogen uptake efficiency in rapeseed (Brassica napus L., cv. Hyola308, a field experiment was conducted as split plot factorial based on randomized complete block design at research field of Faculty of Agricultural Sciences, University of Guilan, Iran, during growing season of 2007-2008. Results showed that urea fertilizer, ZnSO4 fertilizer and biological fertilizer had significant effects on nitrogen uptake and accumulation. Maximum grain yield (2568 kg.ha-1 were obtained in 150kgN + ZnSO4+ bio treatment. Maximum accumulation of nitrogen in rosette stage (4.9% and nitrogen content of grain (3.6% was obtained in 150 kg N.ha-1N + ZnSO4 + bio. Maximum Nitrogen uptake efficiency and nitrogen use efficiency (0.86 and 29.56 kg.kg-1, respectively were obtained in 50 kgN.ha-1N + ZnSO4 + bio. In regard to significant effects of ZnSO4 and biological fertilizer with lower N rate and high nitrogen uptake efficiency of rapeseed, it seems that the ability of uptake and use of nitrogen fertilizers was greater for seed formation in the presence of ZnSO4 and biological fertilizer in rapeseed, cv. Hyola308. The most important of mechanisms of PGPRs is increase the bioavailability of mineral nutrients with biological nitrogen fixation and soluble phosphorus and potassium that lead to economize nitrogen fertilizer in rapeseed production and minimizing environmental pollution risk.

  20. CARACTERIZACIÓN DE BACTERIAS DIAZOTRÓFICAS ASIMBIÓTICAS ASOCIADAS AL EUCALIPTO (Eucalyptus sp. EN CODAZZI, CESAR.

    Directory of Open Access Journals (Sweden)

    Dolly Melissa Obando Castellanos

    2010-09-01

    Full Text Available Se evaluó el efecto de las épocas climáticas (lluvia y sequía y del estrato de la muestra (Suelo rizosférico, raíces y hojas sobre la población de los géneros Azotobacter, Beijerinckia, Derxia, Azospirillum, Herbaspirillum, Gluconacetobacter y Burkholderia en el Eucalipto (Eucalyptus sp.. Así mismo, se evalúo su capacidad en la producción de compuestos indólicos como promotores del crecimiento vegetal y su actividad de reducción de acetileno como indicador de la fijación biológica de nitrógeno. Los resultados no registraron diferencias estadísticas significativas en el test de Tukey (P ≤ 0.05 en la población con respecto a la época climática. Con respecto al estrato de muestra, los aislamientos tentativos a Herbaspirillum sp. y Azospirillum sp. presentaron diferencias significativas en suelo rizosférico y raíces. Se obtuvieron 44 aislamientos de los cuales se agruparon por caracterización fenotípica como: 14 presuntivos del género Beijerinckia sp., 12 de Azotobacter sp., 8 de Derxia sp., 4 de Herbarpirillum sp., 5 de Azospirillum sp., 1 de Gluconacetobacter sp. y 1 de Burkholderia sp. Por su alto potencial fueron seleccionados y criopreservados los aislamientos C27, C26, C25 y C45, las cuales presentaron los mejores valores de eficiencia in vitro, superando valores de producción de las cepas de referencia utilizadas (A. chroococcum (AC-01 y A. brasilense (SP7.

  1. Isolation and identification by 16S rRNA sequence analysis of plant growth-promoting azospirilla from the rhizosphere of wheat.

    Science.gov (United States)

    Ayyaz, Khadija; Zaheer, Ahmad; Rasul, Ghulam; Mirza, Muhammad Sajjad

    2016-01-01

    The main objective of the present study was to isolate phytohormone-producing, phosphate-solubilizing strains of Azospirillum from wheat to be used as inoculants for plant growth promotion. Five Azospirillum strains were isolated from the rhizosphere of field-grown wheat (Triticum aestivum L.), and it was confirmed by BOX-polymerase chain reaction (PCR) that the isolates were different and not re-isolates of the same strain. Sequence analysis of the PCR-amplified 16S rRNA gene indicated that four isolates showed maximum similarity to Azospirillum brasilense and one isolate showed maximum similarity to Azospirillum zeae. This is the first report indicating the presence of an A. zeae like isolate in the wheat rhizosphere in Pakistan. The bacterial isolates were characterized for their plant growth-promoting traits, phosphate solubilization, and indole-3-acetic acid (IAA) production. None of the isolates showed phosphate solubilization activity in the commonly used Pikovskaya medium. However, all strains (except AzoK4) exhibited ability to solubilize tricalcium phosphate (TCP) in modified Pikovskaya medium in which sucrose was replaced by Na-malate, as well as in TCP-supplemented Luria-Bertani (LB) medium. Organic acids, such as acetic, citric, lactic, malic, and succinic acids, were detected in culture supernatants of the tested Azospirillum strains. All strains exhibited ability to produce IAA in the growth medium, except Azospirillum sp. AzoK1. Among the strains tested, the maximum IAA production (30.49±1.04mgL(-1)) and phosphate solubilization (105.50±4.93mgL(-1)) were shown by a pure culture of Azospirillum sp. AzoK2. In pot experiments, single-strain inocula of Azospirillum sp. AzoK1 and AzoK2 improved wheat plant growth. Copyright © 2016. Published by Elsevier Editora Ltda.

  2. CARACTERIZACIÓN DE BACTERIAS DIAZOTRÓFICAS ASIMBIÓTICAS ASOCIADAS AL EUCALIPTO (Eucalyptus sp. EN CODAZZI, CESAR (COLOMBIA

    Directory of Open Access Journals (Sweden)

    DOLLY MELISSA OBANDO CASTELLANOS

    2010-01-01

    Full Text Available Se evaluó el efecto de las épocas climáticas (lluvia y sequía y del estrato de la muestra (suelo rizosférico, raíces y hojas sobre la población de los géneros Azotobacter, Beijerinckia, Derxia, Azospirillum, Herbaspirillum, Gluconacetobacter y Burkholderia en el Euca- lipto (Eucalyptus sp.. Así mismo, se evalúo su capacidad en la producción de com- puestos indólicos como promotores del crecimiento vegetal y su actividad de reducción de acetileno como indicador de la fijación biológica de nitrógeno. Los resultados no registraron diferencias estadísticas significativas en el test de Tukey (P ≤ 0,05 en la población con respecto a la época climática. Con respecto al estrato de muestra, los aislamientos tentativos de Herbaspirillum sp. y Azospirillum sp. presentaron diferencias significativas en suelo rizosférico y raíces. Se obtuvieron 44 aislamientos de los cuales se agruparon por caracterización fenotípica como: 14 presuntivos de Beijerinckia sp., 12 de Azotobacter sp., ocho de Derxia sp., cuatro de Herbarpirillum sp., cinco de Azospirillum sp., uno de Gluconacetobacter sp. y uno de Burkholderia sp. Por su alto potencial fueron seleccionados y criopreservados los aislamientos C27, C26 y C25, las cuales presentaron los mejores valores de eficiencia in vitro, superando valores de producción de las cepas de referencia utilizadas (A. chroococcum (AC1 y A. brasilense (SP7.

  3. CARACTERIZACIÓN DE BACTERIAS DIAZOTRÓFICAS ASIMBIÓTICAS ASOCIADAS AL EUCALIPTO (Eucalyptus sp. EN CODAZZI, CESAR.

    Directory of Open Access Journals (Sweden)

    Divan Baldani Vera Lúcia

    2010-12-01

    Full Text Available Se evaluó el efecto de las épocas climáticas (lluvia y sequía y del estrato de la muestra (Suelo rizosférico, raíces y hojas sobre la población de los géneros Azotobacter, Beijerinckia, Derxia, Azospirillum, Herbaspirillum, Gluconacetobacter y Burkholderia en el Eucalipto (Eucalyptus sp.. Así mismo, se evalúo su capacidad en la producción de compuestos indólicos como promotores del crecimiento vegetal y su actividad de reducción de acetileno como indicador de la fijación biológica de nitrógeno. Los resultados no registraron diferencias estadísticas significativas en el test de Tukey (P ≤ 0.05 en la población con respecto a la época climática. Con respecto al estrato de muestra, los aislamientos tentativos a Herbaspirillum sp. y Azospirillum sp. presentaron diferencias significativas en suelo rizosférico y raíces. Se obtuvieron 44 aislamientos de los cuales se agruparon por caracterización fenotípica como: 14 presuntivos del género Beijerinckia sp., 12 de Azotobacter sp., 8 de Derxia sp., 4 de Herbarpirillum sp., 5 de Azospirillum sp., 1 de Gluconacetobacter sp. y 1 de Burkholderia sp. Por su alto potencial fueron seleccionados y criopreservados los aislamientos C27, C26, C25 y C45, las cuales presentaron los mejores valores de eficiencia in vitro, superando valores de producción de las cepas de referencia utilizadas (A. chroococcum (AC-01 y A. brasilense (SP7.

  4. The auxin-signaling pathway is required for the lateral root response of Arabidopsis to the rhizobacterium Phyllobacterium brassicacearum.

    Science.gov (United States)

    Contesto, Céline; Milesi, Sandrine; Mantelin, Sophie; Zancarini, Anouk; Desbrosses, Guilhem; Varoquaux, Fabrice; Bellini, Catherine; Kowalczyk, Mariusz; Touraine, Bruno

    2010-11-01

    Plant root development is highly responsive both to changes in nitrate availability and beneficial microorganisms in the rhizosphere. We previously showed that Phyllobacterium brassicacearum STM196, a plant growth-promoting rhizobacteria strain isolated from rapeseed roots, alleviates the inhibition exerted by high nitrate supply on lateral root growth. Since soil-borne bacteria can produce IAA and since this plant hormone may be implicated in the high nitrate-dependent control of lateral root development, we investigated its role in the root development response of Arabidopsis thaliana to STM196. Inoculation with STM196 resulted in a 50% increase of lateral root growth in Arabidopsis wild-type seedlings. This effect was completely abolished in aux1 and axr1 mutants, altered in IAA transport and signaling, respectively, indicating that these pathways are required. The STM196 strain, however, appeared to be a very low IAA producer when compared with the high-IAA-producing Azospirillum brasilense sp245 strain and its low-IAA-producing ipdc mutant. Consistent with the hypothesis that STM196 does not release significant amounts of IAA to the host roots, inoculation with this strain failed to increase root IAA content. Inoculation with STM196 led to increased expression levels of several IAA biosynthesis genes in shoots, increased Trp concentration in shoots, and increased auxin-dependent GUS staining in the root apices of DR5::GUS transgenic plants. All together, our results suggest that STM196 inoculation triggers changes in IAA distribution and homeostasis independently from IAA release by the bacteria.

  5. Biofilm formation enables free-living nitrogen-fixing rhizobacteria to fix nitrogen under aerobic conditions.

    Science.gov (United States)

    Wang, Di; Xu, Anming; Elmerich, Claudine; Ma, Luyan Z

    2017-07-01

    The multicellular communities of microorganisms known as biofilms are of high significance in agricultural setting, yet it is largely unknown about the biofilm formed by nitrogen-fixing bacteria. Here we report the biofilm formation by Pseudomonas stutzeri A1501, a free-living rhizospheric bacterium, capable of fixing nitrogen under microaerobic and nitrogen-limiting conditions. P. stutzeri A1501 tended to form biofilm in minimal media, especially under nitrogen depletion condition. Under such growth condition, the biofilms formed at the air-liquid interface (termed as pellicles) and the colony biofilms on agar plates exhibited nitrogenase activity in air. The two kinds of biofilms both contained large ovoid shape 'cells' that were multiple living bacteria embedded in a sac of extracellular polymeric substances (EPSs). We proposed to name such large 'cells' as A1501 cyst. Our results suggest that the EPS, especially exopolysaccharides enabled the encased bacteria to fix nitrogen while grown under aerobic condition. The formation of A1501 cysts was reversible in response to the changes of carbon or nitrogen source status. A1501 cyst formation depended on nitrogen-limiting signaling and the presence of sufficient carbon sources, yet was independent of an active nitrogenase. The pellicles formed by Azospirillum brasilense, another free-living nitrogen-fixing rhizobacterium, which also exhibited nitrogenase activity and contained the large EPS-encapsuled A1501 cyst-like 'cells'. Our data imply that free-living nitrogen-fixing bacteria could convert the easy-used carbon sources to exopolysaccharides in order to enable nitrogen fixation in a natural aerobic environment.

  6. In vitro propagation of fraser photinia using Azospirillum-mediated root development.

    Science.gov (United States)

    Llorente, Berta E; Larraburu, Ezequiel E

    2013-01-01

    Fraser photinia (Photinia × fraseri Dress.) is a woody plant of high ornamental value. The traditional propagation system for photinia is by rooting apical cuttings using highly concentrated auxin treatments. However, photinia micropropagation is an effective alternative to traditional in vivo propagation which is affected by the seasonal supply of cuttings, the long time required to obtain new plants, and the difficulties in rooting some clones.A protocol for in vitro propagation of fraser photinia using the plant growth-promoting ability of some rhizobacteria is described here. Bacterial inoculation is a new tool in micropropagation protocols that improves plant development in in vitro culture. Shoots culture on a medium containing MS macro- and microelements, Gamborg's vitamins (BM), N (6)-benzyladenine (BA, 11.1 μM), and gibberellic acid (1.3 μM) produce well-established explants. Proliferation on BM medium supplemented with 4.4 μM BA results in four times the number of shoots per initial shoot that develops monthly. Consequently, there is a continuous supply of plant material since shoot production is independent of season. Azospirillum brasilense inoculation, after 49.2 μM indole-3-butyric acid pulse treatment, stimulates early rooting of photinia shoots and produces significant increase in root fresh and dry weights, root surface area, and shoot fresh and dry weights in comparison with controls. Furthermore, inoculated in vitro photinia plants show anatomical and morphological changes that might lead to better adaptation in ex vitro conditions after transplanting, compared with the control plants.

  7. The effects of arbuscular mycorrhizal fungus and free living nitrogen fixing bacteria on growth, photosynthesis and yield of corn

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    mohsen jahan

    2009-06-01

    Full Text Available In recent years, biological fertilizers have received special attention by scientists in sustainable and low input agriculture. In order to study the effects of arbuscular mycorrhizal fungi and free living nitrogen fixing bacteria on growth and photosynthesis characteristics of corn in conventional and ecological cropping systems, a field experiment was conducted at the Research Farm of Ferdowsi University of Mashhad during year 2006. A split plots arrangement based on randomized complete block design with three replications was used. Treatments consisted four cropping systems (1- High input conventional system, 2- Medium input conventional system, 3- Low input conventional system and 4- Ecological system and four inoculations (1- Mycorrhiza fungus, Glomus intraradices, 2- Bacteria, Azotobacter paspali and Azospirillum brasilense, 3- Dual inoculation, Fungus plus bacteria, and 4- No-inoculation, control, which were allocated to main plots and sub plots, respectively. All agronomic practices and inputs application during planting and nursing for each of cropping systems were conducted according to regional traditions. Results showed that the effect of inoculation on photosynthesis rates of corn was significant, as the highest photosynthesis rate obtained in dual inoculation. Single inoculation (fungus or bacteria was ranked second. The effect of all inoculations on corn dry matter production was significant and dual inoculation produced the highest dry matter yield. The cropping systems have significant effect on corn yield and the difference between medium input conventional system and high input conventional system was significant, but the high input, low input and ecological cropping systems showed no differences. Inoculants affected the SPAD readings, and dual inoculation showed the highest SPAD readings. This study showed that utilization of low input conventional and ecological systems in combination with use of dual inoculation of

  8. Identification and Quantification of Cause and Effects in Symbiosis of Corn with Arbuscular Mycorrehiza Fungus using Structural Equation Modeling Approach

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    M Jahan

    2015-09-01

    Full Text Available Considering to necessity and importance of determining mycorrhizal symbiosis level and its effects on crop agroecological characteristics as well as other related factors affecting this relationship, beside, the lack of an assured method for this purpose, the present study designed based on new perspectives and insights for determining cause and effects relations, latent variables using structural equation modeling (SEM approach. A field experiment was conducted during 2 years. A split plots arrangement based on randomized complete block design (RCBD with three replications was used. Treatments consisted four cropping systems (high, medium, and low input conventional as well as ecological system and four inoculations (mycorrhizae fungus: Glomus intraradices, plant growth promoting rhizobacteria (PGPR: (Azospirillum brasilense, Azotobacter paspali, dual inoculation: (fungus plus bacteria, and no-inoculation (control, which were allocated to main plots and sub plots, respectively. At the first step, a confirmatory factor analyzing was conducted resulted to two distinguished factors, and then the variables which had the most loads (weight on one of these two factors were determined. At the second step, considering ecophysiological basis of crops growth and development, in order to continue analyzing, the first factor (including: leaf area index (LAI, root length colonization percent (RLCP, dry matter (DM, stem diameter (D, SPAD readings and the second factor (including: maximum photosynthesis (Amax, specific root length (SRL, canopy temperature (CT, plant height (H, soil respiration rate (SRR, variable chlorophyll fluorescence to maximum chlorophyll fluorescence (Fv/Fm, plant tissue phosphorus content (%P determined resource capture latent construct and resource utilization latent construct, respectively. Correlation coefficients, squared multiple correlation coefficients, covariance matrices, direct and indirect path coefficients were calculated

  9. Roman Policies towards Antiochus III and the Greeks from Winter 197/196 B.C. to Autumn 196 B.C.

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    Deutschmann, Eike Hellmut

    2012-01-01

    Full Text Available In the Second Macedonian War (200-196 B.C., the res publica reduced the strength of the enemy King Philip V apparently to establish a new political order in Southern Balkans: Assumedly a pro-Roman balance of forces should prevail there, untainted by influence of another major power. A particular senatorial policy towards the Greeks probably did not exist before the fighting in Hellas came to an end in summer 197 B.C. In the same year, the Seleucid king Antiochus III brought large parts of the west coast of Asia Minor under control and set about crossing the Hellespont. Rome subsequently stylized itself as the guardian of freedom for the Greeks living in Hellas and Asia Minor. The statesmen of the res publica could have perceived Antiochus’ expansion as a threat to the mentioned new order. Therefore, the Roman Policy of Freedom was possibly applied primarily to take action against the Seleucid king. Die res publica verminderte im Zweiten Makedonischen Krieg (200-196 a.c. die Macht des gegnerischen Königs Philipp V - anscheinend um eine neue politische Ordnung im südlichen Balkanraum zu etablieren: Vermutlich sollte dort ein romfreundliches Kräftegleichgewicht vorherrschen, auf das keine andere Großmacht Einfluß hat. Eine speziell an die Griechen gerichtete Politik seitens des römischen Senats gab es wahrscheinlich nicht vor Ende der Kampfhandlungen in Hellas im Sommer 197 a.c. In dem Jahr erweiterte der seleukidische König Antiochos III. seinen Einflussbereich auf große Teile der kleinasiatischen Westküste und schickte sich an, den Hellespont zu überqueren. Rom stilisierte sich in der Folgezeit zum Freiheitsgarant der in Hellas und Kleinasien lebenden Griechen. Antiochos Expansion könnte von den Staatsmännern der res publica als Bedrohung der genannten neuen Ordnung angesehen worden sein. Demzufolge wurde die römische Freiheitspolitik möglicherweise in erster Linie angewendet, um gegen den seleukidischen König vorzugehen.

  10. “New” Nationalism and Autochthony – Tales of Origin as Political Cleavage „Neuer“ Nationalismus und Autochthonie – Ursprungsmythen als politische Konfliktlinie

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    Morten Bøås

    2009-05-01

    Full Text Available The conflicts in Liberia, Eastern DRC and Côte d’Ivoire can be “read” as “wars of modernity” as they are concerned with the composition of their respective polities: who is a citizen and who is not. However, these contemporary conflicts are deeply embedded in a long history of violence: integral to this history is the issue of land. Citizenship in itself does not secure access to land, but at the very least it allows those with this status a legitimate entry to the competition for land. There is therefore a direct link between contested citizenship and land rights issues. Drawing on fieldwork material from the above-mentioned countries, this article will show how localised identity narratives under certain circumstances destroy as well as reformulate national identities, and that insight from ethnographic work on autochthonic issues can help us understand conflicts in a broad range of African countries. Die Konflikte in Liberia, im Osten der Demokratischen Republik Kongo und der Côte d’Ivoire können als „Kriege der Moderne“ angesehen werden, weil es dabei jeweils um den Aufbau des Gemeinwesens geht: Wer ist Bürger und wer nicht? Hinter diesen aktuellen Konflikten steht jedoch eine lange Geschichte der Gewalt, wobei die Landfrage das zentrale Problem darstellt. Staatsangehörigkeit allein ist keine Garantie für das Recht auf Landbesitz; sie legitimiert aber zumindest Personen mit diesem Status zur Teilnahme am Wettbewerb um Landbesitz. Deshalb besteht beim Thema Landbesitz ein direkter Bezug zwischen den Auseinandersetzungen über Staatsbürgerschafts- und Besitzrechtsfragen. Auf der Grundlage von Material aus Feldstudien in den genannten Ländern zeigt dieser Artikel, wie einheimische Identitäts-„Narrative“ in bestimmten Zusammenhängen nationale Identität sowohl zerstören als auch neu bilden, und wie Erkenntnisse zu Autochthonie-Fragen aus ethnografischen Arbeiten helfen, derartige Konflikte in zahlreichen

  11. Theory of sampling and its application in tissue based diagnosis.

    Science.gov (United States)

    Kayser, Klaus; Schultz, Holger; Goldmann, Torsten; Görtler, Jürgen; Kayser, Gian; Vollmer, Ekkehard

    2009-02-16

    A general theory of sampling and its application in tissue based diagnosis is presented. Sampling is defined as extraction of information from certain limited spaces and its transformation into a statement or measure that is valid for the entire (reference) space. The procedure should be reproducible in time and space, i.e. give the same results when applied under similar circumstances. Sampling includes two different aspects, the procedure of sample selection and the efficiency of its performance. The practical performance of sample selection focuses on search for localization of specific compartments within the basic space, and search for presence of specific compartments. When a sampling procedure is applied in diagnostic processes two different procedures can be distinguished: I) the evaluation of a diagnostic significance of a certain object, which is the probability that the object can be grouped into a certain diagnosis, and II) the probability to detect these basic units. Sampling can be performed without or with external knowledge, such as size of searched objects, neighbourhood conditions, spatial distribution of objects, etc. If the sample size is much larger than the object size, the application of a translation invariant transformation results in Kriege's formula, which is widely used in search for ores. Usually, sampling is performed in a series of area (space) selections of identical size. The size can be defined in relation to the reference space or according to interspatial relationship. The first method is called random sampling, the second stratified sampling. Random sampling does not require knowledge about the reference space, and is used to estimate the number and size of objects. Estimated features include area (volume) fraction, numerical, boundary and surface densities. Stratified sampling requires the knowledge of objects (and their features) and evaluates spatial features in relation to the detected objects (for example grey value

  12. Von den Dispositiven der Macht und ihrer sozialen Morphologie - Zur Aktualität der Machtanalysen Michel Foucaults

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    Wolfgang Neurath

    2016-12-01

    Full Text Available Dieser Beitrag rekapituliert im Blick auf die Überlappungen von Macht, Souveränität und Herrschaft die soziale Morphologie der Foucaultschen Gesellschaftstheorie und betont dabei die Aktualität von Forschungen, die sich machtkritisch an den Dispositivbegriff anlehnen. Was ein Dispositiv ist, wird im Rekurs auf Deleuze und Agamben vor Augen geführt, um in der Figur der "schizophrenen Flucht" ein entscheidendes Moment der französischen Debatten auszumachen. Dabei ist der Panoptismus nach wie vor ein bemerkenswertes Modell, um historisch die innere Funktionsweise von Disziplinargesellschaften zu begreifen, die sich zu Kontrollgesellschaften gewandelt haben. Dem Homo digitalis steht angesichts von Big Data im Semiokapitalismus des 21. Jahrhunderts ein System wechselseitiger Kontrolle vor Augen, dem er sich lückenlos unterwerfen soll. Dem setzt sich nach wie vor die Figur der widerständigen Subjektivierung entgegen, wie sie Foucault in seinem Spätwerk entworfen hat. Auf allgemeinster Ebene hebt dieser Artikel hervor, dass wir auch heute noch ein politisches Modell vor Augen haben, dass der antiken Arena im Sinne einer politischen Heterotopie entspricht, in der es im Krieg um das nackte Überleben geht. Zusammenfassend wird Foucaults Kriegsparadigma über Michel de Certeaus Modellierung von Strategie und Taktik auf die Figur des Bürgerkriegs bezogen, um insgesamt zu betonen, dass dort wo Macht ist, immer auch Widerstand möglich ist. This article recaps the social morphology of Foucault’s theory of society in the light of power, sovereignty, and domination, emphasizing the actuality of research that is referring to the concept of apparatus. This concept is pointed out in the recourse to Deleuze and Agamben, to identify – in the figure of "schizophrenic escape" – a decisive moment in the French debates. Panoptism is still a remarkable model for historical understanding of the internal functioning of disciplinary societies that have

  13. Theory of sampling and its application in tissue based diagnosis

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    Kayser Gian

    2009-02-01

    Full Text Available Abstract Background A general theory of sampling and its application in tissue based diagnosis is presented. Sampling is defined as extraction of information from certain limited spaces and its transformation into a statement or measure that is valid for the entire (reference space. The procedure should be reproducible in time and space, i.e. give the same results when applied under similar circumstances. Sampling includes two different aspects, the procedure of sample selection and the efficiency of its performance. The practical performance of sample selection focuses on search for localization of specific compartments within the basic space, and search for presence of specific compartments. Methods When a sampling procedure is applied in diagnostic processes two different procedures can be distinguished: I the evaluation of a diagnostic significance of a certain object, which is the probability that the object can be grouped into a certain diagnosis, and II the probability to detect these basic units. Sampling can be performed without or with external knowledge, such as size of searched objects, neighbourhood conditions, spatial distribution of objects, etc. If the sample size is much larger than the object size, the application of a translation invariant transformation results in Kriege's formula, which is widely used in search for ores. Usually, sampling is performed in a series of area (space selections of identical size. The size can be defined in relation to the reference space or according to interspatial relationship. The first method is called random sampling, the second stratified sampling. Results Random sampling does not require knowledge about the reference space, and is used to estimate the number and size of objects. Estimated features include area (volume fraction, numerical, boundary and surface densities. Stratified sampling requires the knowledge of objects (and their features and evaluates spatial features in relation to

  14. Bilderpolitik - Der gewöhnliche Faschismus im Kontext der Produktion und der Rezeption

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    Sabine Hänsgen

    2016-07-01

    Full Text Available Obyknovennyi fashizm / Der gewöhnliche Faschismus war ein sowjetisches Filmprojekt, das im Jahr 1965 von Michail Romm gemeinsam mit Jurij Chanjutin und Maja Turovskaja auf der Grundlage vom während der NS-Zeit entstandenen Bildmaterial realisiert wurde. Bei dem Projekt handelt es sich um den ersten umfassenden Versuch einer filmischen Reflexion über den Faschismus und implizit auch über die eigene totalitäre Vergangenheit in der nachstalinistischen Sowjetunion. Dieser Schlüsselfilm der 1960er Jahre löste eine breite internationale Diskussion aus, die Einblick in das diskursive Feld der wechselseitigen politisch-ideologischen Spiegelungen während der Zeit des Kalten Krieges gewährt. Obyknovennyi fashizm gehört zum Genre des „Kompilationsfilms“, das eine Wiederveröffentlichung und Neuinterpretation historischen Bildmaterials leistet. Montiert wurde der Film aus NS-Wochenschauen, Dokumentarfilmen und Kulturfilmen (2 Mio Meter Film, die 1945 von der Roten Armee aus den Beständen des Reichsfilmarchivs konfisziert und nach Moskau gebracht worden waren. Dieses Filmmaterial wurde ergänzt durch Fotografien, etwa die Fotoporträts des Hitler-Leibfotografen Heinrich Hoffmann oder private Schnappschüsse von Soldaten der deutschen Wehrmacht, die Jahrzehnte später im Zusammenhang mit der ersten Wehrmachtsausstellung großes Aufsehen erregten. In dem Artikel von Wolfgang Beilenhoff und Sabine Hänsgen werden die langwierige Produktionsgeschichte und die kontroverse Rezeption des Films in BRD und DDR behandelt. Besondere Aufmerksamkeit finden bei der Filmanalyse die medialen Strategien, mit denen dieser Kompilationsfilm das NS-Bildmaterial einer kritischen Lektüre unterzieht. Im Zentrum stehen das Zusammenspiel von visueller Montage und mündlichem Autorenkommentar sowie die Verwendung anderer Medien (Fotografie und Buch im Film. Im Schlussteil des Artikels wird Romms Film im Kontext anderer filmischer Auseinandersetzungen mit

  15. Power-Sharing, Conflict and Transition in Burundi: Twenty Years of Trial and Error Machtteilung, Konflikt und Wandel in Burundi: Zwanzig Jahre Versuch und Irrtum

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    Stef Vandeginste

    2009-01-01

    Full Text Available For the past twenty years, Burundi has experimented with powersharing as an instrument of political liberalisation, democratisation and conflict resolution. This contribution analyses the different meanings the concept of power-sharing has had throughout Burundi’s recent and extremely violent political transition, in particular during the lengthy peace process. It shows how national and international actors have found inspiration in the toolbox of consociationalism to negotiate and design the Arusha Peace and Reconciliation Agreement for Burundi signed in August 2000 and its posttransition Constitution. Power-sharing has been instrumental in achieving the – short-term – objective of war termination. It has also de-ethnicised political competition and reduced the (potentially destabilising effect of elections. Measured against more ambitious state-building objectives (democracy, rule of law, accountable and effective governance, power-sharing has (so far not been able to make a difference. Several factors and developments threaten the “survival” of the power-sharing model in Burundi. In Burundi wird seit zwanzig Jahren mit Power-Sharing als Instrument politischer Liberalisierung, Demokratisierung und Konfliktlösung experimentiert. Der vorliegende Beitrag analysiert, welche unterschiedlichen Bedeutungen das Konzept der Machtteilung in der jüngsten und extrem gewalttätigen Entwicklungsphase Burundis angenommen hat, insbesondere während des langwierigen Friedensprozesses. Er zeigt auf, inwieweit nationale und internationale Akteure sich bei der Aushandlung und Planung des Arusha-Abkommens zu Frieden und Versöhnung in Burundi, das im August 2000 unterzeichnet wurde, sowie der anschließenden Verfassung am Instrumentarium der Konkordanzdemokratie bedient haben. Power-Sharing erwies sich als nützlich, das kurzfristige Ziel einer Beendigung des Krieges zu erreichen, und trug zur De-Ethnisierung des politischen Wettbewerbs und zur

  16. Desempenho agronômico a campo de híbridos de milho inoculados com Azospirillum

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    Patricia Dörr de Quadros

    2014-04-01

    Full Text Available O uso de inoculantes na cultura do milho tem sido cada vez mais valorizado, em vista dos benefícios que pode trazer à cultura, como a fixação biológica do nitrogênio e o aumento da quantidade de raízes. Isto pode melhorar a absorção de água e nutrientes pela planta, contribuindo para o desenvolvimento do milho, principalmente em períodos de seca. Este estudo foi desenvolvido com o objetivo de avaliar, em condições de campo, as características agronômicas e o rendimento de grãos de híbridos de milho, inoculados, ou não, com uma mistura de três espécies de Azospirillum (A. brasilense, A. lipoferum, A. oryzae. Foram avaliados o teor relativo de clorofila nas folhas, a altura de planta, a senescência foliar, os componentes de rendimento de grãos, o teor de N, a matéria seca da parte aérea das plantas e o número mais provável de bactérias diazotróficas na rizosfera das plantas. A inoculação manteve o teor de clorofila significativamente maior até o estádio R3 das plantas, para os três híbridos testados, aumentou o rendimento da matéria seca da parte aérea, dos híbridos AS 1575 e SHS 5050, o peso de 1000 grãos, para o híbrido P32R48 e altura, para o AS 1575. Houve interação entre os genótipos de milho e as bactérias inoculadas, visto que, cada híbrido testado respondeu de forma diferente à inoculação. A inoculação de Azospirillum em milho demonstrou estimular o desenvolvimento das plantas no período vegetativo, aumentando a probabilidade de obter-se um estande de plantas uniforme, maior resistência ao estresse e maior concentração de clorofila nas folhas.

  17. Densidade e diversidade fenotípica de bactérias diazotróficas não simbióticas em solos da Reserva Biológica Serra dos Toledos, Itajubá (MG

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    Talita Filomena Silva

    2011-04-01

    Full Text Available Bactérias diazotróficas não simbióticas (BDNS atuam no desenvolvimento das plantas por meio da fixação biológica de nitrogênio e também pela produção e liberação de substâncias reguladoras do crescimento vegetal. Este estudo objetivou avaliar a densidade e diversidade fenotípica desse grupo de bactérias em fragmentos de solo da Reserva Biológica Serra dos Toledos e entorno, em Itajubá/MG. Essa reserva localiza-se na Área de Proteção Ambiental da Mantiqueira, constituindo-se em uma importante área de recarga e de abrigo à flora e fauna endêmicas. Amostras de solo superficial foram coletadas em áreas com diferentes declividades na reserva, em épocas representativas das estações de inverno (setembro/2006 e verão (abril/2007. A densidade, avaliada pelo número mais provável, utilizando os meios de cultura NFb, JNFb e Fam, para Azospirillum spp., Herbaspirillum spp. e A. amazonense, respectivamente, variou de 0,12 a 75,60 (NMP x 10(5 bactérias g-1 solo seco. Foram obtidos 172 e 174 isolados, respectivamente para as amostras de inverno e verão, dos quais 30 e 55 % apresentaram similaridade igual ou superior a 70 % com as estirpes-tipo Azospirillum brasilense, A. amazonense, A. lipoferum, Herbaspirillum seropedicae e Burkholderia brasilensis. O resultado do comportamento dos isolados com base na tolerância à salinidade nem sempre foi semelhante ao obtido pelas características fenotípicas culturais a 70 % de similaridade, sendo indicado para estudos complementares de diversidade desses organismos. As BDNS apresentam potencial de utilização em estudos de avaliação da qualidade e sustentabilidade de ecossistemas. No entanto, apesar da alta densidade e diversidade fenotípica em solos da reserva, maiores valores foram obtidos no entorno, evidenciando o efeito positivo da cobertura vegetal do tipo gramíneas sobre elas, independentemente da variação climática.

  18. Enhancement of growth and nutrient uptake of rapeseed (Brassica napus L.) by applying mineral nutrients and biofertilizers.

    Science.gov (United States)

    Yasari, Esmaeil; Azadgoleh, M A Esmaeili; Mozafari, Saedeh; Alashti, Mahsa Rafati

    2009-01-15

    For investigating the effect of chemical fertilizer as well as biofertilizers on seed yield and quality i.e. oil, protein and nutrients concentration of rapeseed (Brassica napus L.), a split-plot fertilizers application experimental design in 4 replications was carried out during the 2005-2006 growing season, at the Gharakheil Agricultural Research Station in the Mazandaran province of Iran. Rapeseed was grown as a second crop in rotation after rice. Biofertilizers treatments were two different levels: control (no seed inoculation) and seeds inoculation with a combination of Azotobacter chroococcum and Azosprillum brasilense and Azosprillum lipoferum, as main plot and chemical fertilizers comprised N, P, K and their combinations, NPKS and NPK Zn as sub plots. The maximum value of seed yield obtained at (BF+NPK Zn) 3421.2 kg h(-1) corresponding to 244.5 pods per plant and maximum concentration of Zn in leaves as well as seeds. The highest weight of 1000 seeds (4.45 g) happened to obtain at (BF+NPK S) which coinciding with the maximum K levels in leaves. The highest number of branches was obtained at (BF+NPK Zn) with 4.43 branches per plant i.e., 46.2% increase over the control. The maximum value of rapeseed oil content 47.73% obtained at T16 (BF+NK) but maximum protein concentration of seed obtained at T12 (BF+N). Overall the results indicated that inoculation resulted in increase in seeds yield (21.17%), number of pods per plant (16.05%), number of branches (11.78%), weight of 1000 grain (2.92%), oil content of seeds (1.73%) and protein (3.91%) but decrease (-0.24%) in number of seeds per pods comparing to non-Biofertilizers treatments. Irrespective to the treatments, results showed that application of Biofertilizers coincided with 3.86, 0.82, 2.25, 0.75 and 0.91% increase in concentrations of N, P, K, S and Zn in the seeds over the non-Biofertilizers treatments.

  19. Application effects of biofertilizers on the growth indices of black cumin (Nigella sativa L.

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    sorur khoram del

    2009-06-01

    Full Text Available Application of biofertilizers, especially plant growth promoting rhizobacteria (PGPR and mycorrhiza fungus is one of the most important strategies for plant nutrition compared to chemical fertilizers, especially in sustainable management of agroecosystems. In order to investigate the effect of Azotobacter and Azospirillum bacteria and Mycorrhiza fungus on the growth indices of black cumin (Nigella sativa L., a field experiment was conducted at the Agricultural Research Station of Ferdowsi University of Mashhad during growing season of 2007. A randomized complete block design with three replications was used. Treatments included: (A Azotobacter paspali, (B Azospirillum brasilense, (C the fungus of Glomus intraradaices, C+A, C+B, A+B, A+B+C, and control without using bio-fertilizers. The Azotobacter and Azospirillum inoculations were applied as liquid and the Mycorrhiza inoculation was applied in solid form on the treated seeds with Arabic resin immediately before planting. The Arabic resin was applied to increase the adherence of Mycorrhiza to seeds. In all treatments except control, the amounts of 15 mg of each bio-fertilizer were applied for 110 g of seeds. The results indicated that the inoculation of black cumin with biological fertilizers significantly increased plant height, leaf area index, dry matter accumulation and crop growth rate compared with control. The maximum plant height was observed in Azospirillum+Mycorrhiza at 89 days after emerging. The highest and lowest leaf area index was observed in B+C (0.37 and control (0.22 treatments, respectively. The fast period of vegetative growth and dry matter accumulation were observed at 40-89 days after emerging with a small decline afterwards until physiological maturity. The maximum and minimum amounts of dry matter accumulation were recorded in the B+C treatment with 66.0 gm-2, and control with 38.3 gm-2, respectively. Crop growth rate reached to its peak in 82 days after emergence

  20. BACTÉRIAS DIAZOTRÓFICAS NÃO SIMBIÓTICAS E ENRAIZAMENTO DE ESTACAS SEMILENHOSAS DE OLIVEIRA (Olea europaea L.

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    Talita Filomena Silva

    2017-01-01

    Full Text Available Non-symbiotic diazotrophic bacteria (NSDB influence plant growth mainly through biological nitrogenfixation and production and release of plant growth-regulating substances. The effect of these bacteria on rooting of olive is not described in the international literature, which has been provided by the use of hormonal treatment based on indole butyric acid (IBA. The purpose of this study was to evaluate the physiological diversity of isolates obtained from soil samples of the Biological Reserve Serra dos Toledos, Itajubá, MG state, and of reference strains of NSDB, and their potential of substitution of IBA on rooting of olive semi-hardwood cuttings from the experimental farm ‘Maria da Fé’ (FEMF-EPAMIG. The isolates were divided into five groups (G1 to G5 according with cultural phenotypic characteristics based on color, diameter and consistence of colonies, and reference strains used were Azospirillum brasilense (BR11001 t , Azospirillum amazonense (BR11040 t , Herbaspirillum seropedicae (BR11175 t and Burkholderia brasilensis (BR11340 t . The diversity of physiological was performed using phosphate solubilization, tolerance to different conditions of temperature and pH and production of indole acetic acid (IAA. To evaluate the potential of semi-hardwood cuttings rooting in olive cultivars were used the cultivars Ascolano 315, Arbequina and Grappolo 541, from the germoplasm blank of the FEMF-EPAMIG. There were 33 treatments, and the cuttings were inoculated with the bacterial isolate groups, with the reference strains, without inoculation (control and only IBA, commercially used in rooting. Only two isolates showed ability to solubilize phosphate. Groups of isolates and of reference strains showed very similar environmental requirements, with wide ranges of optimum conditions of pH (5,0-9,0 and temperature (15-35ºC for maximum growth. The isolates and reference strains showed a production IAA ranging from 110.53 to 383.58 μg mL -1 , and

  1. [Johann Laurentius Bausch and Philipp Jacob Sachs of Lewenhaimb. Foundation of the Academia Naturae

    Science.gov (United States)

    Müller, Uwe

    2008-01-01

    The biographical notes of the two municipal physicians of Schweinfurt, Leonhard Bausch (1574 to 1636) and Johann Laurentius Bausch (1605-1665) and another three physicians (Johann Michael Fehr, Georg Balthasar Wohlfarth and Georg Balthasar Metzger) who founded the Academia Naturae Curiosorum together with the younger Bausch in 1652, show that this founding was initiated by a surprisingly homogenous group, sharing the same social, educational and professional background as well as ancestral and acquired experiences. They all had been influenced by the immigration fate of their families, the rapid rise to the politically or academically educated elite in the imperial city of Schweinfurt, worn out by war and plagues. They all had studied at universities in protestant territories of the Holy Roman Empire, finishing with an educational journey (peregrinatio academica), usually to Italy. Experience of the flourishing university life beyond the frontiers of the Holy Roman Empire laid waste by the "Teutsche Krieg", the great variety of academies in Italy, the narrowness of contemporary medicine and the inability of the individual to explore the immense variety of nature: all this leads to the founding of the Academia Naturae Curiosorum and it is the point of reference of the founding documents of 1651/1652, which were first printed in 1662 (Salve Academicum). What is innovative about this is not the establishment of an academy but the desired aim and the way of achieving this. The tenor of these documents--to medically explore the variety of the divine "res naturals" in a cooperative and regulated way for the benefit of medicine and mankind and to publish the results in monographes (utilitas by curiositas)--was condensed by the later Leopoldina to the still used motto "to explore nature for the benefit of mankind". Due to Breslau's municipal physician Philipp Jacob Sachs von Lewenhaimb (1627 - 1672) the publishing activities of the academy came into being. But before the

  2. Construction nationale et construction des identités de genre en Allemagne (1848-1870

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    Alice Primi

    2009-12-01

    Full Text Available L’exemple allemand analysé dans cet article montre comment les catégories du « masculin » et du « féminin » sont construites, transformées et utilisées en lien étroit avec l’identité nationale. Trois périodes – la révolution de 1848-1849, les années 1860 et la guerre de 1870 – sont successivement examinées à travers des textes produits par des Allemandes qui tentent de participer à la formation de l’opinion publique durant ces années-là. Ces sources (principalement de nature journalistique témoignent de leurs tentatives pour intervenir dans les débats du moment, pour exprimer des opinions politiques, pour revendiquer de nouveaux droits pour les femmes et définir pour elles un statut de citoyennes dans l’Etat allemand à venir. Pour se faire entendre, ces auteures doivent se montrer dignes de la nation allemande, et, par conséquent, s’exprimer non seulement « en tant que femmes » mais aussi en tant que « femmes allemandes » ; ceci implique de se distinguer clairement du stéréotype de la « femme française ». La revendication de cette double identité – genrée et nationale – s’avère à la fois indispensable et restrictive, comme on le voit avec les thèmes de l’émancipation et des droits politiques. Beaucoup de ces femmes publiquement engagées essaient donc d’élargir leur champ d’action en jouant sur la notion de « féminité » qu’elles sont obligées de prendre en compte ; il s’agit notamment de donner à cette notion un contenu nouveau et « moderne », en lien avec les problèmes de leur temps.Der Text zeigt anhand der Analyse des deutschen Beispiels auf, wie im Zusammenspiel mit der nationalen Identität Geschlechterkategorien im 19. Jahrhundert konstruiert, verändert und benutzt wurden. Untersucht werden drei Zeiträume: die Revolution von 1848-49, die 1860er Jahre und der deutsch-französische Krieg von 1870/71. Die hier vorgestellten Quellen wurden von

  3. Faculty of social Christian teaching at Jagiellonian University

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    Stanisław Piech

    1989-12-01

    Full Text Available Die Veroffentlichung der Enzyklika "Rerum novarum" im Jahre 1891 weckte allenthalben ein grosses Interesse fur die katholische Soziallehre und regte viele zur sozialen Arbeit an. An den Universitaten wurden vermehrt auch Vortragsreihen zu diesem Fragenkreis angeboten. Die Jagellonen-Universitat, an der bereits im Mittelalter diese Problematik aufgegriffen wurde /vgl. Stanisław aus Skalbmierz und Paweł Włodkowic/, fanden die ersten Veranstaltungen dazu an der Juristischen-Fakultat im Studienjahr 1892/93 statt und seit 1899/1900 auch an der Theologischen Fakultat. Professoren der letzteren stellten 1908 -an das osterreichische Ministerium fur Kultus und Unterricht den Antrag, an der jU einen Lehrstuhl fur die Katholische Soziallehre zu grunden, was 1910 auch erfolgt ist. Der erste Lehrstuhlinhaber und Professor fur die Katholische Soziallehre, Kazimierz Zimmermann, war ein Geistlicher und bekannter Vorkampfer fur das Sozialrecht der polnischen Bewohner Grosspolens. Er gab auch die erste polnische Zeitschrift "Ruch Chrześcijańsko-Społeczny" heraus, die den Fragen der katholischen Soziallehre gewidmet war. Die Grundung des Lehrstuhls fur die Katholische Soziallehre an der Jagellonen-Universitat wurde durch die sozialistische Jugend vehement bekampft und hatte etliche Ausschreitungen zur Folge. Diese Aktion der sozialistisch eingestellten Jugend ging in die Geschichte der JU als "Zimmermann-Krieg" ein. 1926 wurde in dem nunmehr wieder unabhangigen Polen dieser Lehrstuhl durch den Minister fur Kultus und Bildung aubgeschafft. Die Bemuhungen der Bischofe von Krakau, Tschenstochau und OberSchlesien - unterstutzt durch die Theologische Fakultat der JU - um die Wiedereinrichtung des Lehrstuhls zeitigten keinen Erfolg. Angesichts dieser Lage bekam Jan Piwowarczyk, ein Geistlicher und Redaktionsmitglied der Zeitung "Głos Narodu", schon im Studienjahr 1928/ 1929 den Lehrauftrag, uber die Katholische Soziallehre zu lesen. Dann 1934/ 35 ubernahm diese

  4. Berliner Geowissenschaftlerinnen an der Friedrich-Wilhelms-Universität von 1906 bis 1945, eine Fallstudie

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    B. A. R. Mohr

    2003-01-01

    Full Text Available In dieser Untersuchung werden beispielhaft die Lebenswege und Karrieren von Berliner Geowissenschaftlerinnen im Zeitraum von 1906 bis 1945 nachgezeichnet und analysiert. Ähnlich wie an anderen deutschen bzw. westlichen Universitäten, aber im Gegensatz zu Russland, begann die Tätigkeit von Frauen in den Geowissenschaften spät, und das Fach wurde auch relativ selten gewählt, hauptsächlich wegen der zu geringen Berufschancen. Aber die besondere Situation in Berlin mit mehreren sich ergänzenden Institutionen und dem daraus resultierenden breiten Spektrum an geowissenschaftlichen Disziplinen, sowie ausgezeichneten Professoren, ließ dennoch Raum für eine Ausbildung in diesem Bereich und erlaubte, wenn auch in bescheidenem Maße, eine gewisse Karrieremöglichkeit. Während der hier untersuchten 40 Jahre haben weniger als 20 Frauen in den Geowissenschaften und benachbarten Gebieten promoviert. Mehrere dieser Frauen blieben in dem von ihnen gewählten Fach weiterhin aktiv und wurden erfolgreich. Zwei Frauen gelang eine akademische Karriere — eine als Universitätsprofessorin, die auch Schülerinnen hatte. Andere arbeiteten an staatlichen Institutionen, wie z. B. dem Geologischen Landesamt. Wenige Frauen blieben nach ihrer Verheiratung beruflich aktiv, wenn auch nicht offiziell angestellt, sondern als Ehefrauen. Andere arbeiteten als "Ersatz" für die im Kriege stehenden Männer. Einige waren aus persönlichen und politischen Gründen, insbesondere während der NS-Zeit, gezwungen, die Geowissenschaften zu verlassen, konnten aber teilweise auf anderen Gebieten erfolgreich arbeiten. This paper documents the lives and careers of women geoscientists at the Berlin Friedrich-Wilhelms-University from 1906 through 1945. Traditionally, in Germany, women had difficulties to be accepted in geosciences (except for geography/geology teachers, because of strong links between geology and mining, a field dominated clearly by men. In western European countries

  5. Zur Geschichte der Geowissenschaften im Museum für Naturkunde zu Berlin Teil 1: Aus der Vorgeschichte bis zur Gründung der Berliner Bergakademie im Jahre 1770

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    G. Hoppe

    1998-01-01

    Full Text Available Die Geschichte der beiden Institute für Mineralogie und für Paläontologie des Berliner Museums für Naturkunde mit ihren sehr großen Sammlungen beginnt mit ihrer direkten Vorgängerin, der Berliner Bergakademie, die 1770 gegründet worden ist. Aber bereits vor dieser Zeit hat es in Berlin geowissenschaftliche Interessen und Betätigungen gegeben. Diese Vorgeschichte wird mit einer Zeit begonnen, in der es den Ort Berlin noch längst nicht gab. Aus der La-Tene-Zeit, die der Zeit der griechischen Antike entspricht, stammt eine Aschenurne mit einer Sammlung fossiler Mollusken, die im norddeutschen Flachland bei Bernburg gefunden wurde. Die Zusammensetzung dieser Sammlung läßt bereits ein wissenschaftliches Herangehen erkennen. Für Berlin selbst ist kurz nach Georg Agricola eine Persönlichkeit der Renaissance zu verzeichnen, Leonhard Thurneysser zum Thurn, in dessen vielfältigen Aktivitäten auch Mineralien einen Platz hatten. In gleicher Zeit war in Berlin am brandenburgischen Hofe eine Raritätenkammer vorhanden, die spätere Kunst- und Naturalienkammer. Sie existierte bis über das Jahr 1770 hinaus und enthielt auch Mineralien und Versteinerungen. Das sich hierdurch zeigende Interesse an solchen Objekten war noch recht oberflächlich. Erst die Sammlungen privater Personen, die in Berlin seit Ende des 17. Jahrhunderts entstanden sind, zeigen ein tieferes und wissenschaftliches Interesse, wenn auch in verschiedenem Maße und in unterschiedlicher Spezialisierung. Unter ihnen ragt besonders Johann Gottlob Lehmann heraus. Als vielseitiger Naturwissenschaftler und Bergrat hielt er privat Vorlesungen in Mineralogie und Bergbaukunde. Der Siebenjährige Krieg verhinderte den Ausbau und die Fortsetzung. Erst Jahre danach, 1768, reorganisierte König Friedrich II. das preußische Bergwesen und richtete 1770 die Berliner Bergakademie ein. Hierbei kam dem Arzt und Bergrat Carl Abraham Gerhard bei der Einrichtung und als Lehrkraft eine wesentliche

  6. Die Veränderung der Grenzen in einer transnationalen Gebietskörperschaft

    Directory of Open Access Journals (Sweden)

    Karen Denni

    2009-12-01

    konfliktreichen Linie der Trennung während des deutsch-französischen Krieges von 1870-1871 zu einer friedvollen und integrierten Grenzzone innerhalb der Europäischen Union. Die Vereinbarung von 2005 über die Gründung des Eurodistrikts Straßburg-Ortenau sieht sogar die Überwindung politischer und sozialer Grenzen vor, indem langfristig eine Fusion der beiden benachbarten Gebietskörperschaften angestrebt wird. Obwohl diese Vereinbarung zu einer Verstärkung der grenzüberschreitenden Zusammenarbeit geführt hat, sind viele juristische, politische und soziale Fragen noch ungeklärt. Auch die Bürger der Grenzregion sind bisher nur unzureichend in den Entwicklungsprozess des Eurodistrikts einbezogen worden. Es ist daher noch ein langer Weg zu beschreiten, ehe der Rhein nicht mehr die Grenze zwischen Frankreich und Deutschland darstellt, sondern einen Bindestrich zwischen beiden Ländern.

  7. Ekkehart Krippendorff, investigador para la Paz. Una biografía intelectual

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    Francesco Pistolato

    2011-06-01

    Full Text Available Ekkehart Krippendorff, nacido en 1934, es un ilustre investigador para la paz alemán, prácticamente ignoto en el mundo hispano-hablante por falta de estudios sobre él y de traducción de sus obras más importantes. Educado en el nazismo y en la Alemania del Oeste de la posguerra, profundiza su formación en Ciencias Políticas en los EEUU a comienzos de los años Sesenta. Regresado a Alemania, en 1966 publica la antología Political Science, una introducción al estudio de las Ciencias Políticas estadounidenses, en aquella época desconocidas en Alemania.A pesar de su admiración a la democracia estadounidense, Krippendorff critica la política imperialista de los EEUU con el ensayo “Amerikanische Politik in Asien” de 1963 y con Die amerikanische Strategie de 1970. Su posición anticonformista impide su ingreso en la Universidad de Berlín hasta finales de los años Setenta, después de varias experiencias de docencia en los EEUU y en Italia.En los años Ochenta se compromete en el movimiento contra la instalación de los misiles de medio alcance en Alemania. Su obra teórica principal, Staat und Krieg (El Estado y la guerra de 1985 se convierte en un clásico del pacifismo en los países de idioma alemán. En ésta explica que el Estado moderno nació para reglamentar a las tropas mercenarias y garantizar a los poderosos la fuerza necesaria no sólo para defenderse de los ataques externos y para conquistar nuevos territorios, sino para controlar a los ciudadanos de su mismo país. Además de proporcionar varios ejemplos históricos para fundamentar su tesis, la obra demuestra la insensatez de las políticas de potencia con citas de literatos, filósofos y otros estudiosos de ciencias humanas. Un excursus dedicado al Tolstoi pacifista concluye esta obra riquísima e inclasificable, quizás ante todo un tratado sobre la necesidad de desconfiar de los políticos y de participar activamente en la política nacional.En los últimos veinte a

  8. Effect of Biofertilizers and Irrigation Intervals on Yield Component and Yield of Fenugreek (Trigonella foenum- graecum L.

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    M. Jaberi

    2016-02-01

    , Azad University, Birjand, Iran, during 2010-2011. Experimental treatments were irrigation intervals in three levels (every 6, 9 and 12 days and biofertilizer in five levels (nitroxin, biophosphorus, and micorhyza fungi G. mosseae, G. intraradices and control-no fertilizer. Fenugreek was planted through furrow in mid-April 2010. The distance between rows and between plants was 30 and 10 cm, respectively. Seeds were covered by Nitroxin (including: Azotobacterchroococcum, Azospirillum lipoferum and Azospirillum brasilense and biophosphrous (including: Pseudomonas fluorescens and then were cultured.They were used at a depth of 2cm below the seed from Glomusintraradiceand Glomus mosseae. Fenugreek was harvested in early July when the plants became yellow and at least 80% of the pods were reached. Statistical analysis of the data was performed using SAS 9.1 and MSTATC. Mean comparison were compared by the Duncan test at 1% and 5% level of probability. Results and Discussion: Results of statistical analysis showed that irrigation treatments and biofertilizers had significant effect on most indices. Interaction effect of irrigation and biofertilizer were significant on seed and leaf yield (p

  9. Evaluation of the Effects of Bio Fertilizers Containing non Symbiotic Nitrogen Fixing and Phosphate Solubilizing Bacteria on Quantitative and Qualitative Traits of Wheat

    Directory of Open Access Journals (Sweden)

    M Mohtadi

    2016-02-01

    4 levels: 1-noinoculation (B0, 2- Seeds inoculated with nitrogen fixing bacteria (B1, 3-Seed inoculation with phosphate solubilizing bacteria (B2, 4- Combined application of bio-fertilizers (B3. Results and Discussion Analysis of variance showed that grain yield, plant height, leaf area index, yield components, straw weight, biological yield, harvest index, percent of nitrogen and grain protein were influenced by different levels of biological and chemical fertilizers (Table 3. The highest grain yield was obtained using C2B3, combination treatments using chemical fertilizers and biofertilizers (PSB+NFB. The results of interactions between chemical fertilizers and biofertilizers showed the using 100% of the recommendations fertilizer along with biofertilizers (PSB+NFB significantly increased grain yield (Table 5, compared with control. Due to increasing activity of bacteria Aztobacter chroococum, Azospirillium brasilense enhanced nitrogen fixation and released phyto hormones and thereby increased nutrient uptake by the roots. In addition, Pseudomonas Potida and Pantoea agglomerace had beneficial effects beside phosphorus uptake. These bacteria increased absorption and dissolved nutrients in the soil around the roots. PGPRs produced the plant growth regulator, organic acids and increased the ability to absorb elements such as iron, zinc and other micro elements and ultimately were effective in increasing crop yield and percent of nitrogen and grain protein. Conclusions Results of the experiment showed that using phosphate solubilizing bacteria and nitrogen fixing simultaneously or individually increased total plant biomass, grain nitrogen, protein content, yield components and crop yield. However, the combined use of phosphate solubilizing bacteria and nitrogen fixing compared to use of individually was more s effective. A synergic effect was found between chemical fertilizers and biological fertilizers. It is recommended to apply chemical fertilizers along with

  10. A disposable laser print-cut-laminate polyester microchip for multiplexed PCR via infra-red-mediated thermal control

    Energy Technology Data Exchange (ETDEWEB)

    Ouyang, Yiwen [Department of Chemistry, University of Virginia, Charlottesville, VA 22904 (United States); Duarte, Gabriela R.M. [Department of Chemistry, University of Virginia, Charlottesville, VA 22904 (United States); Universidade Federal de Goiás, Goiânia, GO 74690-900 (Brazil); Poe, Brian L.; Riehl, Paul S. [Department of Chemistry, University of Virginia, Charlottesville, VA 22904 (United States); Santos, Fernando M. dos; Martin-Didonet, Claudia C.G. [Universidade Estadual de Goiás, Anápolis, GO 75132-400 (Brazil); Carrilho, Emanuel [Instituto de Química de São Carlos, Universidade de São Paulo, São Carlos, SP 13566-590 (Brazil); Instituto Nacional de Ciência e Tecnologia de Bioanalítica, CP 6154, Campinas, SP 13083-970 (Brazil); Landers, James P., E-mail: landers@virginia.edu [Department of Chemistry, University of Virginia, Charlottesville, VA 22904 (United States); Department of Mechanical Engineering, University of Virginia, Charlottesville, VA 22904 (United States); Department of Pathology, University of Virginia Health Science Center, Charlottesville, VA (United States)

    2015-12-11

    Infrared (IR)-mediated thermal cycling system, a method proven to be a effective for sub-μL scale polymerase chain reaction (PCR) on microchips, has been integrated with DNA extraction and separation on a glass microchip in a fully integrated micro Total Analysis System by Easley et al., in 2006. IR-PCR has been demonstrated on both glass and PMMA microdevices where the fabrication (bonding) is not trivial. Polyester-toner (PeT) microfluidic devices have significant potential as cost-effective, disposable microdevices as a result of the ease of fabrication (∼$0.25 USD and <10 min per device) and availability of commercial substrates. For the first time, we demonstrate here the thermal cycling in PeT microchips on the IR-PCR system. Undesirable IR absorption by the black-toner bonding layer was eliminated with a spatial filter in the form of an aluminum foil mask. The solution heating rate for a black PeT microchip using a tungsten lamp was 10.1 ± 0.7 °C s{sup −1} with a cooling rate of roughly −12 ± 0.9 °C s{sup −1} assisted by forced air cooling. Dynamic surface passivation strategies allowed the successful amplification of a 520 bp fragment of the λ-phage genome (in 11 min) and a 1500 bp region of Azospirillum brasilense. Using a centrosymmetric chamber configuration in a multichamber PeT microchip, homogenous temperature distribution over all chambers was achieved with inter-chamber temperature differences at annealing, extension and denaturing steps of less than ±2 °C. The effectiveness of the multichamber system was demonstrated with the simultaneous amplification of a 390 bp amplicon of human β-globin gene in five PeT PCR microchambers. The relative PCR amplification efficiency with a human β-globin DNA fragment ranged from 70% to 90%, in comparison to conventional thermal cyclers, with an inter-chamber standard deviation of ∼10%. Development of PeT microchips for IR-PCR has the potential to provide rapid, low

  11. ДИПЛОМАТИЧНІ ДОКУМЕНТИ ЯК СФЕРА ФУНКЦІОНУВАННЯ НІМЕЦЬКОЇ ФАХОВTHE DIPLOMATIC DOCUMENTS AS THE FUNCTIONING OF THE GERMAN PROFESSIONAL LANGUAGE OF DIPLOMACYОЇ МОВИ ДИПЛОМАТІЇ /

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    Олена КАМІНСЬКА

    2017-03-01

    also a set of special terms and phrases that generally creates the world’s diplomatic vocabulary. In this sense the language of diplomacy is as specific and professional as, for example, the language of a doctor, a lawyer and so on. Every diplomat clearly interprets and actively uses such concepts and terms (including the ones that came in a modern language from Latin as pacta sunt servant, modus vivendi, veto, kalter Krieg (cold war and others. However, in modern diplomatic correspondence it is not accepted to abuse specificity of the diplomatic language, the openness of the modern diplomacy and presumption of a broad disclosure are considered, and hence the availability of the document being developed. It should be noted that the German language plays a special role in the world politics, as well as in the diplomatic sphere, since it is often used as language intermediary in the communication of different cultures. Кey words: diplomacy, diplomatic document, diplomatic correspondence, cliché, figure of speech, note.

  12. Kirjalikud teated eesti libahundipärimuse kohta kuni 20. sajandini alguseni / Schriftliche Überlieferungen zum Werwolfsglauben in Estland bis Anfang des 20. Jahrhunderts

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    Tiina Vähi

    2011-01-01

    Full Text Available Die Geisteshaltung bei der Beschreibung des Werwolfglaubens ist seit Herodot (484-425 vor Chr. durch das Mittelalter bis zur Frühen Neuzeit relativ unverändert geblieben. Ein Merkmal der Kolonialliteratur ist der Ethnozentrismus bei der Darstellung von in der Peripherie lebenden Heiden bis hin zu deren Dämonisierung. Sebastian Münster (1489-1552 schrieb im fünften Band seines Werkes Cosmographey. Oder Beschreibung Aller Länder. (1544, dass es in Livland zahlreiche Hexen und Werwölfe gebe und Olaus Magnus schrieb in seinem Buch Historia de Gentibus Septentrionalibus (1555, dass Werwölfe in Livland mehr Schaden anrichteten als gewöhnliche Wölfe. Ein schreckenerregendes Bild von Übeltaten von Wölfen oder von in Werwölfe verwandelten Räubern malt der Jesuit Antonio Possevino (1533-1611, Gesandter des Papstes, in seinem Brief an die Herzogin von Mantua (verfasst in Tartu am 9. August 1585. Gegen Ende des Mittelalters erhielt die Tierwandlung, besonders die Verwandlung in einen Wolf, eine äußerst negative Bedeutung, sie wurde mit Lykanthropie in Verbindung gebracht. In einem Abschnitt von Balthasar Russows Chronica der Provintz Lyfflandt (1584, der die mehrwöchige Belagerung der Burg Toolse durch die Schweden 1574 während des Livländischen Krieges beschreibt, heißt es, dass die russischen Verteidiger den Belagerern jeden Abend als heulende Wolfsherde erschienen und die Angreifer so einschüchterten.Auch die aufgeklärten Gelehrten des 18. und beginnenden 19. Jahrhunderts kamen nicht gänzlich von den Konzepten der Vergangenheit los, ein „Werwolf“ drückte den Unglauben des Volkes aus, der von der Ungebildetheit des Volkes herrührte. August Wilhelm Hupel (1737-1819, der in der estnischen Kulturgeschichte als Sammler von Daten zur Geographie, Ethnographie und Bevölkerung Liv- und Estlands geschätzt wird, behandelte im sechsten Teil seines Werkes Nordische Miscellen (1781-1791 den Werwolfglauben, wobei er sich auf frühere von

  13. The Effects of Carboxin-thiram on Associative Relationships between Azospirillum Species and Wheat (Chamran Cultivar

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    F. rejali

    2017-03-01

    Full Text Available Introduction: Biofertilizers have been identified as alternative to chemical fertilizers to increase soil fertility and crop production in sustainable farming systems. One of the most useful kind of biofertilizers include plant growth promoting rhizobacteria (PGPR. Azospirillum is an associative rhizobacteria which can be very useful for plants such as wheat. It can help plant by fixing nitrogen through biological way, causing root development, plant strength improvement in primary phases, causing germination percent increment, improving plant tolerance in stress situations (drought, salinity, soil compaction and pathogens, secreting plant promoting hormones like cytokinin, Oxin and finally yield increment will be observable. Modern agriculture largely relies on the extensive application of agrochemicals, including inorganic fertilizers and pesticides. Although pesticides are important, their effects on nontarget organisms are of great concern because this poses a risk to the entire ecological system. The fungicides may also adversely affect the soil microflora, especially the types of microorganisms that can applied to seeds as bacterial inoculants. Considering useful effects of plant growth promoting rhizobacteria especially Azospirillum on Wheat, this study was done in order to survey interaction effects between fungicide and available biofertilizers in Iran market. Materials and Methods: Effect of carboxin tiram in 2 levels (applied, non-applied as fungicide, on efficacy of wheat plant (Chamran Cultivar and final crop yields under association conditions with 5 Azospirillum species (A.brasilense, A.lipoferum, A.halopraeferense, A.irakense, A.sp using powdery and liquid formulation were studied in a greenhouse test for four months in Soil and Water Research Institute.At first some properties of used soil, including soil texture, pH, EC,organic carbon and available soil K, P, Fe, Zn, Mn and Cu were measured by laboratory methods.Nutrient Broth

  14. EDITORIAL: Van der Waals interactions in advanced materials, in memory of David C Langreth Van der Waals interactions in advanced materials, in memory of David C Langreth

    Science.gov (United States)

    Hyldgaard, Per; Rahman, Talat S.

    2012-10-01

    Solid State Commun. 17 1425 [4]Gunnarsson O and Lundqvist B I 1976 Exchange and correlation in atoms, molecules, and solids by the spin-density-functional formalism Phys. Rev. B 13 4274 [5]Langreth D C and Mehl M J 1981 Beyond the local-density approximation in calculations of ground-state electronic properties Phys. Rev. B 47 446 [6]Dion M, Rydberg H, Schröder E, Langreth D C and Lundqvist B I 2004 Van der Waals density functional for general geometries Phys. Rev. Lett. 92 246401 Thonhauser T, Cooper V R, Li S, Puzder A, Hyldgaard P and Langreth D C 2007 Van der Waals density functional: self-consistent potential and the nature of the van der Waals bond Phys. Rev. B 76 125112 [7]Lee K, Murray E D, Kong L, Lundqvist B I and Langreth D C 2010 A higher-accuracy van der Waals density functional Phys. Rev. B 82 081101 [8]Rapcewicz K and Ashcroft N W 1991 Fluctuation attraction in condensed matter: a nonlocal functional approach Phys. Rev. B 44 4032 Lundqvist B I, Andersson Y, Shao H, Chan S and Langreth D C 1995 Density functional theory including van der Waals forces Int. J. Quant. Chem. 56 247 [9]Langreth D C et al 2009 A density functional for sparse matter J. Phys.: Condens. Matter 21 084203 [10]For example, Kohn W, Meir Y and Makarov D E 1998 The exchange-correlation energy of a metallic surface Phys. Rev. Lett. 80 4153 Kurth S and Perdew J P 1999 Phys. Rev. B 59 10461 Dobson J F and Wang J 1999 Phys. Rev. Lett. 82 2123 Pitarke J M and Perdew J P 2003 Phys. Rev. B 67 045101 Vydrov O A and van Voorhi T 2009 Phys. Rev. Lett. 103 063004 [11]For example, Grimme S 2004 J. Comput. Phys. 25 1463 Tkatchenko A and Scheffler M 2009 Phys. Rev. Lett. 102 073005 Grimme S, Antony J, Ehrlich S and Krieg H 2010 J. Chem. Phys. 132 154004 [12]Burke K 2012 Perspectives on density functional theory J. Chem. Phys. 136 150901 Van der Waals interactions in advanced materials contents Van der Waals interactions in advanced materials, in memory of David C LangrethPer Hyldgaard and Talat S