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  1. Oxidative Stress in BPH

    Directory of Open Access Journals (Sweden)

    Murat Savas

    2009-01-01

    The present study has shown that there were not relationship between potency of oxidative stress and BPH. Further well designed studies should be planned to find out whether the oxidative stress-related parameters play role in BPH as an interesting pathology in regard of the etiopathogenesis. Keywords: benign prostatic hyperplasia, oxidative stress, prostate

  2. Benign prostate hyperplasia (BPH) - resources

    Science.gov (United States)

    Resources - benign prostatic hyperplasia (BPH); Prostate enlargement resources; BPH resources ... The following organizations provide information on benign prostatic hyperplasia ( prostate enlargement ... Urology Care Foundation -- www. ...

  3. [History of laser in BPH therapy].

    Science.gov (United States)

    Grande, Marco; Facchini, Francesco; Moretti, Matteo; Larosa, Michelangelo; Leone, Marco; Ziglioli, Francesco; Pozzoli, Gian Luigi; Frattini, Antonio

    2014-01-01

    Laser technology has been used in the treatment of BPH for more than 15-20 years in order to challenge transurethral resection of the prostate. The aim of this review article is to analyze the evolution of laser in BPH therapy, from early coagulative techniques - progressively abandoned for their elevated postoperative morbidity and unfavorable outcomes - to the newer techniques of vaporization, resection and enucleation of the prostate. A better comprehension of tissue-laser interactions, the improvement of laser technology and a growing clinical experience have lead to the development of different laser systems (Holmium, KTP, Thulium laser) that challenge TURP. Today, HoLEP and, secondarily, PVP are the laser techniques supported by more clinical evidences and represent valid alternatives to TURP.

  4. Map-based Cloning and Characterization of the BPH18 Gene from Wild Rice Conferring Resistance to Brown Planthopper (BPH) Insect Pest

    Science.gov (United States)

    Ji, Hyeonso; Kim, Sung-Ryul; Kim, Yul-Ho; Suh, Jung-Pil; Park, Hyang-Mi; Sreenivasulu, Nese; Misra, Gopal; Kim, Suk-Man; Hechanova, Sherry Lou; Kim, Hakbum; Lee, Gang-Seob; Yoon, Ung-Han; Kim, Tae-Ho; Lim, Hyemin; Suh, Suk-Chul; Yang, Jungil; An, Gynheung; Jena, Kshirod K.

    2016-01-01

    Brown planthopper (BPH) is a phloem sap-sucking insect pest of rice which causes severe yield loss. We cloned the BPH18 gene from the BPH-resistant introgression line derived from the wild rice species Oryza australiensis. Map-based cloning and complementation test revealed that the BPH18 encodes CC-NBS-NBS-LRR protein. BPH18 has two NBS domains, unlike the typical NBS-LRR proteins. The BPH18 promoter::GUS transgenic plants exhibited strong GUS expression in the vascular bundles of the leaf sheath, especially in phloem cells where the BPH attacks. The BPH18 proteins were widely localized to the endo-membranes in a cell, including the endoplasmic reticulum, Golgi apparatus, trans-Golgi network, and prevacuolar compartments, suggesting that BPH18 may recognize the BPH invasion at endo-membranes in phloem cells. Whole genome sequencing of the near-isogenic lines (NILs), NIL-BPH18 and NIL-BPH26, revealed that BPH18 located at the same locus of BPH26. However, these two genes have remarkable sequence differences and the independent NILs showed differential BPH resistance with different expression patterns of plant defense-related genes, indicating that BPH18 and BPH26 are functionally different alleles. These findings would facilitate elucidation of the molecular mechanism of BPH resistance and the identified novel alleles to fast track breeding BPH resistant rice cultivars. PMID:27682162

  5. The Expression of Lactoferrin in BPH-EPS, BPH-free EPS and Prostate Cancer Secretion

    Institute of Scientific and Technical Information of China (English)

    Xu Kexin; Wang Xiaofeng; Hou Shukun; Wang Yunchun

    2003-01-01

    To examine the expression of lactoferrin(Lf) in both the expressed prostatic secretion(EPS)of BPHpatients,normal males and the secretion of prostate cancer cell lines PC-3 and DU145.The potential correlation of Lf with prostaticcarcinogenesis was also investigated.Methods:Forty EPS samples obtained from 20 BPH patients and 20 normal males as well as thesecretions of prostate cancer cell lines PC-3 and DU145 were subjected to two-dimensional gel electrophoresis(2-DE).Massspectrometry was performed to confirm the nature of the expressed proteins in EPS and prostatic cancer secretion. Results:Based on theresulting electrophoretograms and the followed mass spectrometry analysis,several differentially expressed proteins were detected andthe up-regulation of Lf (MW 35KDa,pI 7-7.5)in BPH-EPS,compared with BPH-free EPS,was also observed. More importantly, Lf wasabsent in prostate cancer cell lines PC-3 and DU145.Conclusion:The results indicate Lf may be produced specifically by benignprostatic epithelium and prostate lost its Lf secretion during the process of carcinogenesis.

  6. Physician perceptions of sexual dysfunction related to benign prostatic hyperplasia (BPH) symptoms and sexual side effects related to BPH medications.

    Science.gov (United States)

    Seftel, A; Rosen, R; Kuritzky, L

    2007-01-01

    In a large-scale epidemiology study, 50% of aging men reported erectile dysfunction (ED) or ejaculatory dysfunction (EjD), with lower urinary tract symptoms (LUTS) an independent risk factor for each of these conditions. In light of the shift from urologists (UROs) to primary care/internal medicine physicians (PCPs) for the initial management of men with LUTS associated with benign prostatic hyperplasia (BPH), a survey was conducted to assess the perceptions of UROs and PCPs regarding sexual dysfunction (SD) in men with LUTS/BPH and the effects of BPH treatments (alpha(1)-adrenergic receptor antagonists (alpha-blockers) and 5alpha-reductase inhibitors (5ARIs)) on sexual function. The survey was mailed to 7500 UROs and 2500 PCPs, with 1275 (13%) surveys returned (1087 by UROs, 177 by PCPs and 11 by other specialty). Alpha-blocker monotherapy was the most common medication prescribed by both UROs (56%) and PCPs (47%). UROs estimated that 19% of their patients with LUTS/BPH experienced SD owing to their symptoms compared with the estimate of 27% by PCPs. UROs estimated that 19% of their patients experienced SD owing to their BPH medication compared with the PCP estimate of 24%. The incidence of EjD owing to BPH medications estimated by UROs (32%) was higher than that estimated by PCPs (22%); the rate of ED estimated by PCPs (34%) was higher than that estimated by UROs (23%). UROs were more aware than PCPs of the specific sexual side effects caused by alpha-blockers versus 5ARIs. These results suggest that physicians are underestimating the prevalence of SD in men with LUTS/BPH. As men with LUTS/BPH are at increased risk for SD, physicians should be especially cognizant of BPH treatment-related sexual side effects.

  7. Does Inflammation Mediate the Obesity and BPH Relationship?

    DEFF Research Database (Denmark)

    Fowke, Jay H; Koyama, Tatsuki; Fadare, Oluwole;

    2016-01-01

    BACKGROUND: BPH is a common disease associated with age and obesity. However, the biological pathways between obesity and BPH are unknown. Our objective was to investigate biomarkers of systemic and prostate tissue inflammation as potential mediators of the obesity and BPH association. METHODS......: Participants included 191 men without prostate cancer at prostate biopsy. Trained staff measured weight, height, waist and hip circumferences, and body composition by bioelectric impedance analysis. Systemic inflammation was estimated by serum IL-6, IL-1β, IL-8, and TNF-α; and by urinary prostaglandin E2...... composition scales, systemic inflammation, and prostate tissue inflammation against BPH outcomes, including prostate size at ultrasound and LUTS severity by the AUA-symptom index (AUA-SI). RESULTS: Prostate size was significantly associated with all obesity measures. For example, prostate volume was 5.5 to 9...

  8. Management of the complications of BPH/BOO

    Directory of Open Access Journals (Sweden)

    Mark J Speakman

    2014-01-01

    Full Text Available Most men will develop histological BPH if they live long enough. Approximately, half will develop benign prostatic enlargement (BPE and about half of these will get BOO with high bladder pressures and low flow, this in turn leads to detrusor wall hypertrophy. Many of these men will only have lower urinary tract symptoms (LUTS but a significant number will also suffer the other complications of BPH. These include urinary retention (acute and chronic, haematuria, urinary tract infection, bladder stones, bladder wall damage, renal dysfunction, incontinence and erectile dysfunction. Recognition of the complications of BPH/BOO early allows more effective management of these complications. This is particularly important for the more serious urinary infections and also for high-pressure chronic retention (HPCR. Complications of LUTS/BPH are very rare in clinical trials because of their strict inclusion and exclusion criteria but are more common in real life practice.

  9. Development of Interpretable Predictive Models for BPH and Prostate Cancer

    Science.gov (United States)

    Bermejo, Pablo; Vivo, Alicia; Tárraga, Pedro J; Rodríguez-Montes, JA

    2015-01-01

    BACKGROUND Traditional methods for deciding whether to recommend a patient for a prostate biopsy are based on cut-off levels of stand-alone markers such as prostate-specific antigen (PSA) or any of its derivatives. However, in the last decade we have seen the increasing use of predictive models that combine, in a non-linear manner, several predictives that are better able to predict prostate cancer (PC), but these fail to help the clinician to distinguish between PC and benign prostate hyperplasia (BPH) patients. We construct two new models that are capable of predicting both PC and BPH. METHODS An observational study was performed on 150 patients with PSA ≥3 ng/mL and age >50 years. We built a decision tree and a logistic regression model, validated with the leave-one-out methodology, in order to predict PC or BPH, or reject both. RESULTS Statistical dependence with PC and BPH was found for prostate volume (P-value < 0.001), PSA (P-value < 0.001), international prostate symptom score (IPSS; P-value < 0.001), digital rectal examination (DRE; P-value < 0.001), age (P-value < 0.002), antecedents (P-value < 0.006), and meat consumption (P-value < 0.08). The two predictive models that were constructed selected a subset of these, namely, volume, PSA, DRE, and IPSS, obtaining an area under the ROC curve (AUC) between 72% and 80% for both PC and BPH prediction. CONCLUSION PSA and volume together help to build predictive models that accurately distinguish among PC, BPH, and patients without any of these pathologies. Our decision tree and logistic regression models outperform the AUC obtained in the compared studies. Using these models as decision support, the number of unnecessary biopsies might be significantly reduced. PMID:25780348

  10. Non‑Hormonal treatment of BPH/BOO

    Directory of Open Access Journals (Sweden)

    Nadir I Osman

    2014-01-01

    Full Text Available Objectives: To review the use of non-hormonal pharmacotherapies in the treatment of lower urinary tract symptoms (LUTS due to presumed benign prostatic hyperplasia (BPH. Materials and Methods: A search of the PUBMED database was conducted for the terms BPH, LUTS, bladder outlet obstruction, alpha-adrenoceptor blockers, anti-muscarinics, and phosphodiesterase-5-inhibitors. Results: Medical therapy has long been established as the accepted standard of care in the treatment of male LUTS. The aim of treatment is improvement in symptoms and quality of life whilst minimizing adverse effects. The agents most widely used as 1 st line therapy are alpha-blockers (AB, as a standalone or in combination with 2 other classes of drug; 5-α reductase inhibitors and anti-muscarinics. AB have rapid efficacy, improving symptoms and flow rate in a matter of days, these effects are then maintained over time. AB do not impact on prostate size and do not prevent acute urinary retention or the need for surgery. Anti-mucarinics, alone or in combination with an AB are safe and efficacious in the treatment of bothersome storage symptoms associated with LUTS/BPH. Phosphodiesterase-5 inhibitors are an emerging treatment option that improve LUTS without improving flow rates. Conclusions: AB are the most well-established pharmacotherapy in the management of men with LUTS/BPH. The emergence of different classes of agent offers the opportunity to target underlying pathophysiologies driving symptoms and better individualize treatment.

  11. PDE-5 Inhibitors for BPH-Associated LUTS.

    Science.gov (United States)

    Brousil, Philip; Shabbir, Majid; Zacharakis, E; Sahai, Arun

    2015-01-01

    Lower urinary tract symptoms associated with benign prostatic hyperplasia (BPH-LUTS) are a highly prevalent problem, and with considerable burden to quality of life. Evidence has emerged that a strong correlation exists in men suffering both BPH-LUTS and erectile dysfunction (ED). Phosphodiesterase type 5 inhibitors (PDE5i) have been shown to be highly effective in treating ED and more recently there is evidence that men with LUTS also benefit. In this review article we discuss the common pathogenic pathways of ED and LUTS, the scientific basis of PDE5i use, the efficacy of PDE5i in LUTS either as monotherapy or in combination with other established medications used in LUTS.

  12. Physical mapping of Bph3, a brown planthopper resistance locus in rice

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    Jirapong Jairin

    2007-09-01

    Full Text Available Resistance to brown planthopper (BPH, a destructive phloem feeding insect pest, is an important objective in rice breeding programs in Thailand. The broad-spectrum resistance gene Bph3 is one of the major BPH resistance genes identified so far in cultivated rice and has been widely used in rice improvement programs. This resistance gene has been identified and mapped on the short arm of chromosome 6. In this study, physical mapping of Bph3 was performed using a BC3F3 population derived from a cross between Rathu Heenati and KDML105. Recombinant BC3F3 individuals with the Bph3 genotype were determined by phenotypic evaluation using modified mass tiller screening at the vegetative stage of rice plants. The recombination events surrounding the Bph3 locus were used to identify the co-segregate markers. According to the genome sequence of Nipponbare, the Bph3 locus was finally localized approximately in a 190 kb interval flanked by markers RM19291 and RM8072, which contain twenty-two putative genes. Additional phenotypic experiment revealed that the resistance in Rathu Heenati was decreased by increasing nitrogen content in rice plants through remobilization of nitrogen. This phenomenon should be helpful for identifying the Bph3 gene.

  13. Contribution of Orexin to the Neurogenic Hypertension in BPH/2J Mice.

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    Jackson, Kristy L; Dampney, Bruno W; Moretti, John-Luis; Stevenson, Emily R; Davern, Pamela J; Carrive, Pascal; Head, Geoffrey A

    2016-05-01

    BPH/2J mice are a genetic model of hypertension associated with an overactive sympathetic nervous system. Orexin is a neuropeptide which influences sympathetic activity and blood pressure. Orexin precursor mRNA expression is greater in hypothalamic tissue of BPH/2J compared with normotensive BPN/3J mice. To determine whether enhanced orexinergic signaling contributes to the hypertension, BPH/2J and BPN/3J mice were preimplanted with radiotelemetry probes to compare blood pressure 1 hour before and 5 hours after administration of almorexant, an orexin receptor antagonist. Mid frequency mean arterial pressure power and the depressor response to ganglion blockade were also used as indicators of sympathetic nervous system activity. Administration of almorexant at 100 (IP) and 300 mg/kg (oral) in BPH/2J mice during the dark-active period (2 hours after lights off) markedly reduced blood pressure (-16.1 ± 1.6 and -11.0 ± 1.1 mm Hg, respectively;Phypertension during the dark period in BPH/2J mice.

  14. NF-κB and androgen receptor variant expression correlate with human BPH progression

    DEFF Research Database (Denmark)

    Austin, David C; Strand, Douglas W; Love, Harold L;

    2016-01-01

    BACKGROUND: Benign prostatic hyperplasia (BPH) is a common, chronic progressive disease. Inflammation is associated with prostatic enlargement and resistance to 5α-reductase inhibitor (5ARI) therapy. Activation of the nuclear factor-kappa B (NF-κB) pathway is linked to both inflammation and ligand......-independent prostate cancer progression. METHODS: NF-κB activation and androgen receptor variant (AR-V) expression were quantified in transition zone tissue samples from patients with a wide range of AUASS from incidental BPH in patients treated for low grade, localized peripheral zone prostate cancer to advanced......-V expression as well as cellular growth and differentiation were assessed. RESULTS: Canonical NF-κB signaling was found to be upregulated in late versus early stage BPH, and to be strongly associated with non-insulin dependent diabetes mellitus. Elevated expression of AR-variant 7 (AR-V7), but not other AR...

  15. Inflammatory Responses in a Benign Prostatic Hyperplasia Epithelial Cell Line (BPH-1) Infected with Trichomonas vaginalis.

    Science.gov (United States)

    Kim, Sang-Su; Kim, Jung-Hyun; Han, Ik-Hwan; Ahn, Myoung-Hee; Ryu, Jae-Sook

    2016-04-01

    Trichomonas vaginalis causes the most prevalent sexually transmitted infection worldwide. Trichomonads have been detected in prostatic tissues from prostatitis, benign prostatic hyperplasia (BPH), and prostate cancer. Chronic prostatic inflammation is known as a risk factor for prostate enlargement, benign prostatic hyperplasia symptoms, and acute urinary retention. Our aim was to investigate whether T. vaginalis could induce inflammatory responses in cells of a benign prostatic hyperplasia epithelial cell line (BPH-1). When BPH-1 cells were infected with T. vaginalis, the protein and mRNA of inflammatory cytokines, such as CXCL8, CCL2, IL-1β, and IL-6, were increased. The activities of TLR4, ROS, MAPK, JAK2/STAT3, and NF-κB were also increased, whereas inhibitors of ROS, MAPK, PI3K, NF-κB, and anti-TLR4 antibody decreased the production of the 4 cytokines although the extent of inhibition differed. However, a JAK2 inhibitor inhibited only IL-6 production. Culture supernatants of the BPH-1 cells that had been incubated with live T. vaginalis (trichomonad-conditioned medium, TCM) contained the 4 cytokines and induced the migration of human monocytes (THP-1 cells) and mast cells (HMC-1 cells). TCM conditioned by BPH-1 cells pretreated with NF-κB inhibitor showed decreased levels of cytokines and induced less migration. Therefore, it is suggested that these cytokines are involved in migration of inflammatory cells. These results suggest that T. vaginalis infection of BPH patients may cause inflammation, which may induce lower urinary tract symptoms (LUTS).

  16. Testosterone Replacement Therapy and BPH/LUTS. What is the Evidence?

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    Baas, Wesley; Köhler, Tobias S

    2016-06-01

    Contrary to the previous dogma that prostatic growth is directly proportional to testosterone levels, emerging research has suggested a lack of testosterone may be a risk factor for lower urinary tract symptoms (LUTS) and benign prostatic hyperplasia (BPH). Within this review article, we have demonstrated the current understanding of the physiology of hypogonadism and its interplay with prostatic and lower urinary tract physiology. The current evidence suggests that not only does testosterone replacement therapy (TRT) not worsen LUTS, but that hypogonadism itself is an important risk factor for LUTS/BPH.

  17. Elemental concentration analysis in PCa, BPH and normal prostate tissues using SR-TXRF

    Energy Technology Data Exchange (ETDEWEB)

    Leitao, Roberta G.; Anjos, Marcelino J.; Canellas, Catarine G.L.; Lopes, Ricardo T., E-mail: roberta@lin.ufrj.b [Coordenacao dos Programas de Pos-graduacao de Engenharia (COPPE/UFRJ), Rio de Janeiro, RJ (Brazil). Lab. de Instrumentacao Nuclear; Correia, Rodrigo C., E-mail: marcelin@lin.ufrj.b [Universidade do Estado do Rio de Janeiro (UERJ), Rio de Janeiro, RJ (Brazil). Inst. de Fisica; Palumbo Junior, Antonio; Souza, Pedro A.V.R.; Nasciutti, Luiz E., E-mail: nasciutt@ufrj.b [Universidade Federal do Rio de Janeiro (UFRJ), RJ (Brazil). Centro de Ciencias da Saude. Lab. de Interacoes Celulares; Ferreira, Luiz C., E-mail: luiz.ferreira@ipec.fiocruz.b [Fundacao Oswaldo Cruz (FIOCRUZ), Rio de Janeiro, RJ (Brazil)

    2009-07-01

    Prostate cancer (PCa) is one of the main causes of illness and death all over the world. In Brazil, prostate cancer currently represents the second most prevalent malignant neoplasia in men, representing 21% of all cancer cases. Benign Prostate Hyperplasia (BPH) is an illness prevailing in men above the age of 50, close to 90% after the age of 80. The prostate presents a high zinc concentration, about 10-fold higher than any other body tissue. In this work, samples of human prostate tissues with cancer (PCa), BPH and normal tissue were analyzed utilizing the total reflection X-ray fluorescence spectroscopy using synchrotron radiation technique (SRTXRF) to investigate the differences in the elemental concentrations in these tissues. SR-TXRF analyses were performed at the X-Ray fluorescence beamline at Brazilian National Synchrotron Light Laboratory (LNLS), in Campinas, Sao Paulo. It was possible to determine the concentrations of the following elements: P, S, K, Ca, Fe, Cu, Zn, Br and Rb. By using Mann-Whitney U test it was observed that almost all elements presented concentrations with significant differences alpha = 0.05) between the groups studied. The elements and groups were: S, K, Ca, Fe, Zn, Br and Rb (PCa X Normal); S, Fe, Zn and Br (PCa X BPH); K, Ca, Fe, Zn, Br and Rb (BPH X Normal). (author)

  18. Prostatic Artery Embolization (PAE) for Symptomatic Benign Prostatic Hyperplasia (BPH): Part 1, Pathological Background and Clinical Implications.

    Science.gov (United States)

    Sun, Fei; Crisóstomo, Verónica; Báez-Díaz, Claudia; Sánchez, Francisco M

    2016-01-01

    Pathological features of benign prostatic hyperplasia (BPH) dictate various responses to prostatic artery embolization (PAE). Typically, BPH originates in the transition zone and periurethral region, where should be considered the primary target area in PAE procedures. Given that histological heterogeneity of components in hyperplasia nodules, epithelial or stromal, identifying the more responsive nodules to PAE will have clinical implications. Since some lower urinary tract symptoms (LUTS) in patients with BPH are usually related to bladder outlet obstruction-induced changes in bladder function rather than to outflow obstruction directly, proper selection of candidate patients prior to PAE is of great clinical importance. BPH is a typical chronic progressive condition, suggesting PAE could aim not only to relieve LUTS but also to delay or prevent the clinical progression. Awareness of the pathological background of BPH is essential for interventional radiologists to improve clinical outcomes and develop new treatment strategies in clinical practice of PAE.

  19. Prostatic Artery Embolization (PAE) for Symptomatic Benign Prostatic Hyperplasia (BPH): Part 1, Pathological Background and Clinical Implications

    Energy Technology Data Exchange (ETDEWEB)

    Sun, Fei, E-mail: feisun@ccmijesususon.com; Crisóstomo, Verónica, E-mail: crisosto@ccmijesususon.com; Báez-Díaz, Claudia, E-mail: cbaez@ccmijesususon.com; Sánchez, Francisco M., E-mail: msanchez@ccmijesususon.com [Jesús Usón Minimally Invasive Surgery Centre (Spain)

    2016-01-15

    Pathological features of benign prostatic hyperplasia (BPH) dictate various responses to prostatic artery embolization (PAE). Typically, BPH originates in the transition zone and periurethral region, where should be considered the primary target area in PAE procedures. Given that histological heterogeneity of components in hyperplasia nodules, epithelial or stromal, identifying the more responsive nodules to PAE will have clinical implications. Since some lower urinary tract symptoms (LUTS) in patients with BPH are usually related to bladder outlet obstruction-induced changes in bladder function rather than to outflow obstruction directly, proper selection of candidate patients prior to PAE is of great clinical importance. BPH is a typical chronic progressive condition, suggesting PAE could aim not only to relieve LUTS but also to delay or prevent the clinical progression. Awareness of the pathological background of BPH is essential for interventional radiologists to improve clinical outcomes and develop new treatment strategies in clinical practice of PAE.

  20. Androgen regulated genes in human prostate xenografts in mice: relation to BPH and prostate cancer.

    Directory of Open Access Journals (Sweden)

    Harold D Love

    Full Text Available Benign prostatic hyperplasia (BPH and prostate carcinoma (CaP are linked to aging and the presence of androgens, suggesting that androgen regulated genes play a major role in these common diseases. Androgen regulation of prostate growth and development depends on the presence of intact epithelial-stromal interactions. Further, the prostatic stroma is implicated in BPH. This suggests that epithelial cell lines are inadequate to identify androgen regulated genes that could contribute to BPH and CaP and which could serve as potential clinical biomarkers. In this study, we used a human prostate xenograft model to define a profile of genes regulated in vivo by androgens, with an emphasis on identifying candidate biomarkers. Benign transition zone (TZ human prostate tissue from radical prostatectomies was grafted to the sub-renal capsule site of intact or castrated male immunodeficient mice, followed by the removal or addition of androgens, respectively. Microarray analysis of RNA from these tissues was used to identify genes that were; 1 highly expressed in prostate, 2 had significant expression changes in response to androgens, and, 3 encode extracellular proteins. A total of 95 genes meeting these criteria were selected for analysis and validation of expression in patient prostate tissues using quantitative real-time PCR. Expression levels of these genes were measured in pooled RNAs from human prostate tissues with varying severity of BPH pathologic changes and CaP of varying Gleason score. A number of androgen regulated genes were identified. Additionally, a subset of these genes were over-expressed in RNA from clinical BPH tissues, and the levels of many were found to correlate with disease status. Our results demonstrate the feasibility, and some of the problems, of using a mouse xenograft model to characterize the androgen regulated expression profiles of intact human prostate tissues.

  1. Androgen regulated genes in human prostate xenografts in mice: relation to BPH and prostate cancer.

    Science.gov (United States)

    Love, Harold D; Booton, S Erin; Boone, Braden E; Breyer, Joan P; Koyama, Tatsuki; Revelo, Monica P; Shappell, Scott B; Smith, Jeffrey R; Hayward, Simon W

    2009-12-21

    Benign prostatic hyperplasia (BPH) and prostate carcinoma (CaP) are linked to aging and the presence of androgens, suggesting that androgen regulated genes play a major role in these common diseases. Androgen regulation of prostate growth and development depends on the presence of intact epithelial-stromal interactions. Further, the prostatic stroma is implicated in BPH. This suggests that epithelial cell lines are inadequate to identify androgen regulated genes that could contribute to BPH and CaP and which could serve as potential clinical biomarkers. In this study, we used a human prostate xenograft model to define a profile of genes regulated in vivo by androgens, with an emphasis on identifying candidate biomarkers. Benign transition zone (TZ) human prostate tissue from radical prostatectomies was grafted to the sub-renal capsule site of intact or castrated male immunodeficient mice, followed by the removal or addition of androgens, respectively. Microarray analysis of RNA from these tissues was used to identify genes that were; 1) highly expressed in prostate, 2) had significant expression changes in response to androgens, and, 3) encode extracellular proteins. A total of 95 genes meeting these criteria were selected for analysis and validation of expression in patient prostate tissues using quantitative real-time PCR. Expression levels of these genes were measured in pooled RNAs from human prostate tissues with varying severity of BPH pathologic changes and CaP of varying Gleason score. A number of androgen regulated genes were identified. Additionally, a subset of these genes were over-expressed in RNA from clinical BPH tissues, and the levels of many were found to correlate with disease status. Our results demonstrate the feasibility, and some of the problems, of using a mouse xenograft model to characterize the androgen regulated expression profiles of intact human prostate tissues.

  2. Contribution of Diagnostic Tests and Drug Therapy in Screening of Benign Prostatic Hyperplasia (BPH in Western Algerian Hospital

    Directory of Open Access Journals (Sweden)

    Abdelkrim Berroukche

    2014-12-01

    Full Text Available The incidence of benign prostatic hyperplasia (BPH is known through the American and European continents whereas it remains much to make to know the epidemiologic profile of this pathology in Algeria. This study aims to show a contribution of diagnostic tests and drug therapy in screening of BPH in Western Algerian hospital. Our study was carried out on 385 men recruited in the Urology department of Saida hospital during the period 2010-2013, consulting for urologic problems whose 120 patients, aged between 45-84 years, have BPH. BPH frequency was 31.1 %. It was prevalent in the age – specific range of 65-74 years. The physical, biological and histological examinations revealed that 75 % of the patients were diagnosed by digital rectal examination (DRE, 70 % have serum-TPSA level lower than 4 ng/ml and 71.2 % have a histological type named "prostatic hyperplasia adeno-fibro-Leio myomatic (or PHAFLM". As for an assumption of responsibility concerning BPH patients 52.7 % have chosen a medical treatment. This work showed the proportion of the subjects carrying BPH in this area of Algeria. This study provides information on the incidence of BPH in this area of Algeria. But more work needs to come to reinforce the results to uncover the various risk factors (age, race, diet, hormones that are behind the development of this disease.

  3. Effect of testicular irradiation on established BPH in the dog: evidence of a non-steroidal testicular factor for BPH maintenance

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    Grayhack, J.T.; Lee, C.; Brand, W.

    1985-12-01

    Irradiation of the testes of four to 13-year-old male beagles with benign prostatic hyperplasia (BPH) was undertaken to attempt to evaluate the possibility that the testes secrete a non-androgenic accessory sex gland-stimulating substance that may have a critical role in the development of BPH. Thirteen non-irradiated and shoulder irradiated control and 16 beagles subjected to 1500 to 2200 rads single dose testis irradiation had pre-irradiation, interval post-irradiation and terminal caliper measurements of prostatic length, width and depth, prostatic and testicular biopsies, and determination of serum testosterone and estradiol levels. Four beagles survived in a group observed for 109 weeks post testis irradiation, 12 in a group observed for 51 and 10 in a group observed for 59 weeks. The wet weight of the prostate was determined at sacrifice. Ratios of the final/initial length and width and final actual/initial calculated weight of the prostate were significantly decreased in testis-irradiated as compared to control beagles. Histologic evaluation also demonstrated a significant difference in degree of prostatic stimulation in control and testis-irradiated groups. The serum testosterone and estradiol levels were not significantly different in the testis-irradiated and control beagles.

  4. Current Pharmacological Treatment for Male LUTS due to BPH: Dutasteride or Finasteride?

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    Pirozzi, Luisella; Sountoulides, Petros; Castellan, Pietro; Presicce, Fabrizio; Lombardo, Riccardo; Romero, Marilena; De Nunzio, Cosimo; Tubaro, Andrea; Schips, Luigi; Cindolo, Luca

    2015-01-01

    Benign prostatic hyperplasia (BPH) is a potentially progressive disease which is commonly associated with bothersome lower urinary tract symptoms (LUTS) and might result in complications, such as acute urinary retention and BPH-related surgery. In the current medical therapy scenario for LUTS attributed to BPH, only one class of drugs, 5-α reductase inhibitors (5ARIs), has been found to be effective in reducing the risk of disease progression. The two 5ARIs that are currently available include finasteride and dutasteride. These two drugs have different pharmacokinetic and pharmacodynamic properties. Greater suppression of dehydrotestosterone is achieved by dutasteride (>90% dutasteride vs 70% finasteride) which theoretically should correlate with greater efficacy in alleviating urinary symptoms. Unfortunately, this hypothesis has not yet been clinically demonstrated. The pertinent literature is scarce and heterogeneous and produces low scientific levels of evidence. The present review article aims to evaluate the comparative head-to-head studies in order to evaluate if the hypothetical clinical differences between dutasteride and finasteride do exist. Pharmacological treatment with either drug results in similar symptom improvements; however dutasteride seems to have a better profile in reducing the risk of prostate surgery and acute urinary retention (AUR). More studies are necessary to better evaluate both the clinical and pharmacoeconomic profile of the two 5ARIs.

  5. Contribution of Diagnostic Tests and Drug Therapy in Screening of Benign Prostatic Hyperplasia (BPH) in Western Algerian Hospital

    OpenAIRE

    Abdelkrim Berroukche; Abderahmane Labani; Mohamed Terras

    2014-01-01

    The incidence of benign prostatic hyperplasia (BPH) is known through the American and European continents whereas it remains much to make to know the epidemiologic profile of this pathology in Algeria. This study aims to show a contribution of diagnostic tests and drug therapy in screening of BPH in Western Algerian hospital. Our study was carried out on 385 men recruited in the Urology department of Saida hospital during the period 2010-2013, consulting for urologic problems whose 120 patien...

  6. A STUDY ON EXPRESSION OF ARmRNA IN HUMEN BPH TISSUE WITH IN SITU RT-PCR

    Institute of Scientific and Technical Information of China (English)

    邱曙东; 霍涌玮; 张秋养; 葛玲

    2002-01-01

    Objecive To check and compare the expression le vels of human androgen receptor (AR) mRNA in both normal adult prostate (NAP) an d benign prostate hyperplasia (BPH) tissues, and to try to find if BPH results f rom the abnormal transcription of ARmRNA in prostate. Methods Expression of the human ARmRNA in 14 paraffin-embedded prosta te tissues (4 cases of NAP and 10 cases of BPH) was studied with the direct in situ reverse transcription-polymerase chain reaction (RT-PC R). Quantitative analysis of the ARmRNA products was performed using the image a nalysis system. Results ①Specific ARmRNA was detected in bo th NAP and BPH speci mens and in both epithelia and interstitial cells. The positive products were re latively densely localized in the cytoplasm of perinuclear zone. ② The intensit y of ARmRNA signals in epithelial cells was significantly stronger than that in interstitial cells (P<0.001). However, there was no statistically significa nt difference in ARmRNA level between NAP and BPH; ③ The heterogeneity of ARmRN A signal and the androgen-independent cells were observed in prostatic epitheli a. Stronger positive signals of ARmRNA were shown in a few basal-cell layer (BC L) cells of BPH tissue, but were not found in that of NAP tissue. Conclusion Results of this study show that there is no signifi cant difference in the ARmRNA expression between NAP and BPH groups in both epit helium and interstitial cells. It may indicate that BPH does not result from the ARmRNA transcription in the prostate.

  7. A STUDY ON EXPRESSION OF ARmRNA IN HUMEN BPH TISSUE WITH IN SITU RT-PCR

    Institute of Scientific and Technical Information of China (English)

    2002-01-01

    Objecive:To check and compare the expression levels of human androgen receptor(AR) mRNA in both normal adult prostate(NAP)and benign prostate hyperplasia(BPH) tissues,and to try to find if BPH results from the abnormal transcription of ARmRNA in prostate.Methods:Expression of the human ARmRNA in 14 paraffinembedded prostate tissues(4 cases of NAP and 10 cases of BPH)was studied with the direct in situ reverse transcription-polymerase chain reaction(RT-PCR).Quantitative analysis of the ARmRNA products was performed using the image analysis system.Results:(1)Specific ARmRNA was detected in both NAP and BPH specimens and in both epithelia and interstitial cells,The positive products were relatively densely localized in the cytoplasm of perinuclear zone.(2)The intensity of ARmRNA signals in epithelial cells was significantly stronger than that in interstitial cells(P<0.001).However,there was no statistically significant difference in ARmRNA level between NAP and BPH;(3)The heterogeneity of ARmRNA signal and the androgen-independent cells were observed in prostatic epithelia.Stronger positive signals of ARmRNA were shown in a few basal-cell layer(BCL) cells of BPH tissue,but were not found in that of NAP tissue,Conclusion:Results of this study show that there is no significant difference in the ARmRNA expression between NAP and BPH groups in both epithelium and interstitial cessl,It may indicate that BPH does not result from the ARmRNA transcription in the prostate.

  8. Contrast-enhanced transrectal ultrasound for assessing vascularization of hypoechoic BPH nodules in the transition and peripheral zones: comparison with pathological examination.

    Science.gov (United States)

    Yang, Jing Chun; Tang, Jie; Li, Yanmi; Fei, Xiang; Shi, Huaiyin

    2008-11-01

    The purpose of this study was to investigate the vascularization of zonal location of hypoechoic benign prostatic hyperplasia (BPH) nodules and to evaluate the clinical value of contrast-enhanced transrectal ultrasound (CETRUS) for assessing vascularity of hypoechoic BPH nodules. Sixty-two patients with hypoechoic biopsy-proven BPH nodules in transition zone (TZ) (32 patients) or peripheral zone (PZ) (30 patients) of the prostate gland underwent CETRUS examination between January 2006 and September 2006. The enhancement characteristics of hypoechoic BPH nodules were observed and time to enhancement (AT), time to peak intensity (TTP) and peak intensity (PI) were measured with ACQ time-intensity curve analysis software. In addition, microvessel density (MVD) and vascular endothelial growth factor (VEGF) immunoreactivity were determined in the biopsy specimens. Microvessels were identified by immunohistochemical staining of endothelial cells for CD34. Findings were compared between hypoechoic BPH nodules located in PZ and TZ. The most common enhancement characteristic of hypoechoic BPH nodules in PZ was nonenhanced area inside (21/30), while most of hypoechoic BPH nodules in TZ appeared homogeneous enhancement (28/32). The average AT and TTP were significantly longer, the average PI was significantly lower in hypoechoic BPH nodules located in PZ than TZ (p BPH nodules located in TZ than PZ (p BPH nodules. Hypoechoic BPH nodules located in PZ and TZ showed significant difference in vascularization, which indirectly verified our finding that BPH nodule could occur in the peripheral zone. CETRUS could afford information on the vascularity of hypoechoic BPH nodules in a noninvasive manner and this could be used to improve selection of nodules for biopsy.

  9. Chronic tempol prevents hypertension, proteinuria, and poor feto-placental outcomes in BPH/5 mouse model of preeclampsia.

    Science.gov (United States)

    Hoffmann, Darren S; Weydert, Christine J; Lazartigues, Eric; Kutschke, William J; Kienzle, Martha F; Leach, Jenny E; Sharma, Jennifer A; Sharma, Ram V; Davisson, Robin L

    2008-04-01

    Recently we described a mouse model, BPH/5, that spontaneously develops the hallmark clinical features of preeclampsia. BPH/5 exhibit impaired placentation before the onset of hypertension and proteinuria, supporting a causal role for the placenta in the pathogenesis of preeclampsia. Here we tested the hypothesis that an increase in reactive oxygen species (ROS) early in pregnancy results in placental abnormalities leading to the maternal symptoms of preeclampsia. We further hypothesized that chronic antioxidant therapy would ameliorate both feto-placental abnormalities and maternal symptoms. ROS levels measured by dihydroethidium revealed significant increases in oxidative stress in BPH/5 placentas at midgestation compared with C57 controls. This increase in ROS was correlated with reduced expression and activity of cytoplasmic superoxide dismutase in early and midgestation BPH/5 placentas. These abnormalities in placental oxidant factors occurred before the onset of maternal symptoms, suggesting a possible causal link between increased ROS and maternal and feto-placental pathology in this model. In support of this, chronic treatment of BPH/5 with the superoxide dismutase-mimetic Tempol throughout gestation significantly improved fetal growth and survival. Furthermore, Tempol ameliorated pregnancy-induced increases in blood pressure and proteinuria in BPH/5 mothers. We confirmed that Tempol radical was present in plasma, and it normalized ROS levels in all placental zones in BPH/5. These data for the first time demonstrate an important causative role for increased ROS in the placenta in the pathogenesis of preeclampsia in a model that spontaneously develops the disease. The results also strongly suggest the potential utility of antioxidant therapy in treating preeclampsia.

  10. Differential proteome analysis of conditioned medium of BPH-1 and LNCaP cells

    Institute of Scientific and Technical Information of China (English)

    CHEN Wen-zheng; PANG Bo; YANG Bo; ZHOU Jian-guang; SUN Ying-hao

    2011-01-01

    Background Although the introduction of serum prostate-specific antigen (PSA) measurements into clinical practice has revolutionized the care of patients with prostate cancer,there are well-recognized limitations of PSA,and there is a critical need to identify additional prostate cancer biomarkers to assist in early detection and prognosis.In this regard,high resolution proteomic technology has the unexceptionable superiority to find those high abundance biomarkers.The purpose of this study was to search new tumor markers by proteomic technology.Methods The proteins in conditioned medium (CM) of BPH-1 and LNCaP cells were profiled by two-dimensional electrophoresis and identified by matrix-assisted laser desorption ionization mass spectrometry (MALDI-TOF-MS).The corresponding mRNA levels of some identified proteins were analyzed by RT-PCR.Results Totally 11 differentially expressed proteins (6 up-regulated including creatine kinase,brain (CKB),triosephosphate isomerase 1 (TPI1),isocitrate dehydrogenase 2 (IDH2) and 5 down-regulated including glutathione S-transferase pi (GST-pi)) in the CM were identified using MALDI-TOF-MS and database search.The expression pattern between mRNA and CM protein levels of CKB,IDH2,TPI1 and GST-pi in BPH-1 and LNCaP was similar.Conclusion We proved a feasible and effective way to search new tumor markers by a proteomics-based strategy and identified 11 potentially useful proteins in CM of BPH-1 and LNCaP cells to distinguish prostate cancer from benign prostatic hypertrophy.

  11. Low Intraprostatic DHT Promotes the Infiltration of CD8+ T Cells in BPH Tissues via Modulation of CCL5 Secretion

    Directory of Open Access Journals (Sweden)

    Yu Fan

    2014-01-01

    Full Text Available Clinical studies suggested thatandrogen might be associated with infiltrating T cells in prostate of benign prostatic hyperplasia (BPH patients, but detail of T-cell subset and mechanism still remained unclear. The present study tested the hypothesis that intraprostatic 5α-dihydrotestosterone (DHT exerts effects on T cells recruitment by BPH epithelial cells. Prostate tissues from 64 cases of BPH patients after transurethral resection of prostate (TURP were divided into 2 groups: (1 no medication history; (2 administration of 5α-reductase type II inhibitor-finasteride 5 mg daily for at least 6 months before surgery. Group 2 presented significantly higher CD8+ T cells infiltration than group 1, but no changes in CD4+ T cells (immunohistochemistry and flow cytometry. In vitro study more CD8+ T cell migrated to the prostate tissue lysates from group 2 and BPH-1 cells in low DHT condition. Transcription of chemokine (C-C motif Ligand 5 (CCL5 mRNA in BPH-1 cells and chemokine (C-C motif receptor 5 (CCR5 mRNA in CD8+ T cells were upregulated in low DHT condition (q-PCR. CCL5 expression was also identified to be higher in group 2 prostate tissues by IHC. This study suggested that intraprostatic DHT may participate in regulating inflammatory response which was induced by human prostatic epithelial cell, via modulating CCL5 secretion.

  12. Synthesis and Crystal Structure of a Neodymium Ionic Associate [Nd(dmso)7Cl][Bph4]2

    Institute of Scientific and Technical Information of China (English)

    2000-01-01

    The title compound, [Nd(dmso)7Cl][Bph4]2(dmso=dimethylsulfoxide, Bph4=tetraphenylboron anion) was synthesized in organic solvent acetone/EtOH and the structure was determined by single-crystal X-ray diffraction. The crystal is triclinic with space group P, and the crystal data are as follows: a=1.2554(1) nm, b=1.2799(1) nm, c=2.4598(3) nm; α=93.53(1)°, β=104.50(1)°, γ=112.77(1)°, V=3.4722(6) nm3, Z=2, Mr=1365.01, Dc=1.306 g·cm-3. The crystal is composed of a big cation [Nd(dmso)7Cl]2+ and two big anions [BPh4]-. The Nd3+ is coordinated by a chlorine atom and seven oxygen atoms from seven dimethylsulfoxides forming a distorted square antiprism coordination polyhedron,while the BPh-4 anion is charge-equilibrium ion.

  13. Growth and characterization of benzaldehyde 4-nitro phenyl hydrazone (BPH) single crystal: A proficient second order nonlinear optical material

    Science.gov (United States)

    Saravanan, M.; Abraham Rajasekar, S.

    2016-04-01

    The crystals (benzaldehyde 4-nitro phenyl hydrazone (BPH)) appropriate for NLO appliance were grown by the slow cooling method. The solubility and metastable zone width measurement of BPH specimen was studied. The material crystallizes in the monoclinic crystal system with noncentrosymmetric space group of Cc. The optical precision in the whole visible region was found to be excellent for non-linear optical claim. Excellence of the grown crystal is ascertained by the HRXRD and etching studies. Laser Damage Threshold and Photoluminescence studies designate that the grown crystal contains less imperfection. The mechanical behaviour of BPH sample at different temperatures was investigated to determine the hardness stability of the grown specimen. The piezoelectric temperament and the relative Second Harmonic Generation (for diverse particle sizes) of the material were also studied. The dielectric studies were executed at varied temperatures and frequencies to investigate the electrical properties. Photoconductivity measurement enumerates consummate of inducing dipoles due to strong incident radiation and also divulge the nonlinear behaviour of the material. The third order nonlinear optical properties of BPH crystals were deliberate by Z-scan method.

  14. Mitochondria-dependent apoptogenic activity of the aqueous root extract of Croton membranaceus against human BPH-1 cells.

    Science.gov (United States)

    Afriyie, D K; Asare, G A; Bugyei, K; Lin, J; Peng, J; Hong, Z

    2015-01-15

    Croton membranaceus aqueous root extract (CMARE) is among the widely used phytotherapeutics in Ghana for the management of benign prostatic hyperplasia (BPH) and prostate cancer. However, the mechanism of action of CMARE remains to be elucidated. This study aimed to establish whether apoptosis is involved in the antiproliferative effect of CMARE on human BPH-1 cells. We determined the effect of treatment with 0, 1, 3, and 5 mg/mL CMARE for 24, 48, and 72 h on the viability and morphology of BPH-1 cells using the MMT assay and phase-contrast microscopy, respectively. We examined the apoptosis-inducing effects of CMARE after 48 h at the cellular level using Hoescht 33258 and JC-1 dye staining and flow cytometry analysis. We performed reverse transcription polymerase chain reaction and Western blotting to confirm the apoptotic effects of CMARE at the molecular level. CMARE induced a significant dose-dependent inhibition in the proliferation of BPH-1 cells (P < 0.05) and an alteration in their morphology and a reduction their density. Furthermore, CMARE induced dose-dependent staining of the nuclear chromatin, significant DNA fragmentation with G₀/G₁ sub-diploid cells (P < 0.01), and loss of the mitochondrial membrane potential in the treated cells compared to the controls after 48 h (P < 0.01). Additionally, while CMARE induced a significant upregulation of the mRNA and protein levels of Bax, those of Bcl2 did not change significantly. Therefore, induction of mitochondria-dependent apoptosis of BPH-1 cells may be a possible mechanism of action of CMARE.

  15. Possible factors influencing high serum Prostate-specific Antigen (PSA in Indonesian patients with Benign Prostatic Hyperplasia (BPH

    Directory of Open Access Journals (Sweden)

    Djoko Rahardjo

    2001-03-01

    Full Text Available Benign prostatic hyperplasia (BPH cases in Indonesia frequently associated with high serum prostate specific antigen (PSA. To explore possible factors that could increase serum PSA level, we performed a retrospective, cross-sectional study on 805 consecutive patients in Sumber Waras and Dr. Cipto Mangunkusumo Hospitals from 1994 to 1997. Clinical manifestations were evaluated and prostate biopsies were performed if indicated. Complete histopathological data were only available in 82 BPH patients with no urinary retention from 1998-1999 and a thin section of paraffin blocks of BPH patients which still could be found from 1994-1999 was analyzed using flow cytometer to obtain the S-phase fraction as a parameter of proliferative activity, From 805 patients, 461 (57% presented with urinary retention and need to be catheteized. Catheteization significantly increased PSA level if compared to noncatheterized patients (16.3 vs. 6,8 ng/mL, p= 0,000. Another data of 82 uncatheteized patients from 1998-1999 has revealed that 79 patients (96.3% had chronic prostatitis and 19 (23.2% showed the presence of prostatic-intraepithelial neoplasia (PIN with an increase of PSA level (5.4 ng/mL. The S-phase fraction of BPH without PIN cases was significantly higher in cases with PSA > 4 ng/ml than patients with PSA ≤ 4 ng/ml (I3.1% vs. 8.9%, p=0,008. As conclusion, the high serum PSA level was mostly due to urethral catheteization and increased prostate volume. There was a tendency of increasing PSA in subclinical inflammation and PIN. Cases with high PSA also showed high proliferative activities which is suggestive of mitogenic activity. (Med J Indones 2001; 10:22-8Keywords: BPH, high PSA, PIN, proliferative activity, s-phase fraction

  16. Silodosin is effective for treatment of LUTS in men with BPH: a systematic review

    Institute of Scientific and Technical Information of China (English)

    Hui Ding; Wan Du; Zi-Zhen Hou; Han-Zhang Wang; Zhi-Ping Wang

    2013-01-01

    The aim of this study was to systematically review the evidence on the efficacy and safety of silodosin treatments on lower urinary tract symptoms (LUTS) in men with benign prostatic hyperplasia (BPH) from randomized controlled trials.We searched PubMed (1966-December 2011),Embase (1974-December 2011) and the Cochrane Library Database (2011,Issue 12).The assessed outcome measures were the change from baseline for the International Prostate Symptom Score (IPSS),quality of life (QoL) score,peak urine maximum flow rate (Qmax),QoL related to urinary symptoms and adverse effects.Two authors independently assessed the study quality and extracted data.All data were analysed using RevMan 5.1.The meta-analysis included four randomized controlled trials with a total of 2504 patients.The study durations were each 12 weeks.At the follow-up end points,the pooled results showed that the change from baseline for the silodosin group was significantly higher than the placebo group for the IPSS,QoL score and Qmax(mean difference (MD)=-2.78,P<0.00001; MD=-0.42,P=0.004; MD=1.17,P<0.00001,respectively) and patients felt more satisfied with QoL related to urinary symptoms in the silodosin group than the placebo group.Ejaculation disorder was the most commonly reported adverse effect.The pooled results also showed that the silodosin group was superior to the 0.2 mg tamsulosin group with respect to the IPSS and QoL score (IPSS:MD=-1.14,P=0.02; QoL score:MD=-0.26,P=0.02) and inferior to the 0.2 mg tamsulosin group with respect to Qmax (MD=-0.85,P=0.01).In contrast,there was no significant difference in the incidence of ejaculation disorder and dizziness between the silodosin and 0.2 mg tamsulosin groups.The current meta-analysis suggested that silodosin is an effective therapy for LUTS in men with BPH and is not inferior to 0.2 mg tamsulosin.

  17. Photoselective green-light laser vaporisation vs.TURP for BPH: meta-analysis

    Institute of Scientific and Technical Information of China (English)

    Hui Ding; Wan Du; Ze-Ping Lu; Zhen-Xing Zhai; Han-Zhang Wang; Zhi-Ping Wang

    2012-01-01

    This study sought to evaluate the efficacy and safety of photoselective vaporisation (PVP) vs.transurethral resection of the prostate (TURP) for patients with benign prostatic hyperplasia (BPH).Eligible studies were identified from electronic databases (Cochrane Library,PubMed and EMBASE).The database search,quality assessment and data extraction were performed independently by two reviewers.Efficacy (primary outcomes:maximum urinary flow rate (Qmax),international prostate symptom score (IPSS),postvoid residual urine (PVR) and quality of life (QoL); secondary outcomes:operative time,hospital time and catheter removal time) and safety (complications,such as transfusion and capsular perforation) were explored by using Review Manager 5.0.Six randomized controlled trials (RCTs) and five case-controlled studies of 1398 patients met the inclusion criteria.A meta-analysis of the extractable data showed that there were no differences in IPSS,Qmax,QoL or PVR between PVP and TURP (mean difference (MD):prostate sizes < 70 ml,Qmax at 24 months,MD=0.01,P=0.97; IPSS at 12 months,MD=0.18,P=0.64; QoL at 12 months,MD=-0.00,P=0.96; PVR at 12 months,MD=0.52,P=0.43; prostate sizes >70 ml,Qmax at 6 months,MD=-3.46,P=0.33; IPSS at.6 months,MD=3.11,P=0.36; PVR at 6 months,MD=25.50,P=-0.39).PVP was associated with a shorter hospital time and catheter removal time than TURP,whereas PVP resulted in a longer operative time than TURP.For prostate sizes < 70 ml,there were fewer transfusions,capsular perforations,incidences of TUR syndrome and clot retentions following PVP compared with TURP.These results indicate that PVP is as effective and safe as TURP for BPH at the mid-term patient follow-up,in particular for prostate sizes <70 ml.Due to the different energy settings available for green-light laser sources and the higher efficiency and performance of higher-quality lasers,large-sample,long-term RCTs are required to verify whether different energy settings affect outcomes.

  18. A case-based evaluation of SRD5A1, SRD5A2, AR, and ADRA1A as candidate genes for severity of BPH.

    Science.gov (United States)

    Klotsman, M; Weinberg, C R; Davis, K; Binnie, C G; Hartmann, K E

    2004-01-01

    In men with a clinical diagnosis of benign prostatic hyperplasia (BPH), polytomous logistic regression analysis was conducted to evaluate associations between two silent polymorphisms in SRD5A1 (codon positions 30 and 116), two polymorphisms in SRD5A2 (Val89Leu substitution and C to T transition in intron 1), a trinucleotide (CAG)n repeat in androgen receptor (AR), and an Arg492Cys substitution in ADRA1A and clinical parameters that characterize severity of BPH. Candidate gene selection was based on two mechanistic pathways targeted by pharmacotherapy for BPH: (1) androgen metabolic loci contributing to prostate growth (static obstruction); and (2) factors affecting smooth muscle tone (dynamic obstruction). Polymorphisms in SRD5A2 were not associated with severity of BPH; however, SRD5A1 polymorphisms were associated with severity of BPH. The process(es) in which these silent single-nucleotide polymorphisms (SNPs) influence BPH phenotypes is unknown and additional studies will be needed to assess whether these SNPs have direct functional consequences. The characterization of additional molecular factors that contribute to static and dynamic obstruction may help predict response to pharmacotherapy and serve to identify novel drug targets for the clinical management of BPH.

  19. BPH Procedural Treatment: The Case for Value-Based Pay for Performance

    Directory of Open Access Journals (Sweden)

    Mark Stovsky

    2008-01-01

    Full Text Available The concept of “pay for performance” (P4P applied to the practice of medicine has become a major foundation in current public and private payer reimbursement strategies for both institutional and individual physician providers. “Pay for performance” programs represent a substantial shift from traditional service-based reimbursement to a system of performance-based provider payment using financial incentives to drive improvements in the quality of care. P4P strategies currently embody rudimentary structure and process (as opposed to outcomes metrics which set relatively low-performance thresholds. P4P strategies that align reimbursement allocation with “free market” type shifts in cognitive and procedural care using evidence-based data and positive reinforcement are more likely to produce large-scale improvements in quality and cost efficiency with respect to clinical urologic care. This paper reviews current paradigms and, using BPH procedural therapy outcomes, cost, and reimbursement data, makes the case for a fundamental change in perspective to value-based pay for performance as a reimbursement system with the potential to align the interests of patients, physicians, and payers and to improve global clinical outcomes while preserving free choice of clinically efficacious treatments.

  20. A Crystallizable dinuclear tuck-in-tuck-over tuck-over dialkyl Tren uranium complex and double dearylation of BPh(4)(-) to give the BPh(2)-functionalized metallocycle [U{N(CH(2)CH(2)NSiMe(3))(2)(CH(2)CH(2)NSiMe(2)CHBPh(2))}(THF)].

    Science.gov (United States)

    Gardner, Benedict M; McMaster, Jonathan; Lewis, William; Blake, Alexander J; Liddle, Stephen T

    2009-08-05

    The unprecedented formation of a dinuclear tuck-in-tuck-over tuck-over dialkyl Tren-uranium(IV) complex and the first example of double dearylation of BPh(4)(-) in a molecular context to give a BPh(2)-functionalized uranium metallocycle are reported.

  1. Risk of Prostate Cancer after Trans Urethral Resection of BPH: A Cohort and Nested Case-Control Study

    Energy Technology Data Exchange (ETDEWEB)

    Karlsson, Camilla T., E-mail: Camilla.thellenberg@onkologi.umu.se [Department of Radiation Sciences, Oncology, Umeå University, SE-901 87 Umeå (Sweden); Wiklund, Fredrik; Grönberg, Henrik [Department of Medical Epidemiology and Biostatistics, Karolinska Institute, Stockholm SE-171 77 (Sweden); Bergh, Anders [Department of Medical Biosciences, Pathology, Umeå University, SE-901 87 Umeå (Sweden); Melin, Beatrice [Department of Radiation Sciences, Oncology, Umeå University, SE-901 87 Umeå (Sweden)

    2011-11-08

    Epidemiological and experimental evidence suggests that inflammation plays a role in both prostate cancer (PCa) and benign prostate hyperplasia (BPH). This study evaluates the risk of PC after transurethral resection (TURP) for BPH and estimates the PCa risk related to presence of inflammation in the resected material. The Pathology Department at the University Hospital of Umeå (Umeå, Sweden) identified BPH cases (n = 7,901) that underwent TURP between 1982 and 1997. Using these pathological specimens, we compared the incidence of PCa in the cohort to the population and calculated the standardized incidence and mortality ratios (SIR and SMR). Inflammation, the androgen receptor (AR), and p53 were evaluated in a nested case-control study of 201 cases and controls. Inflammation was graded severe or mild-moderate. In the follow-up period after TURP, cases developed prostate cancer and the controls did not. After TURP, SIR for prostate cancer increased [1.26, CI 95% (1.17–1.35) ], whereas SMR decreased [0.59, CI 95% (0.47–0.73) ]. Presence of inflammation at the time of TURP did not differ between cases and controls nor were there differences in p53 or AR staining. The data suggest a small increased risk of PCa after TURP and decreased PCa mortality. Inflammation at the time of TURP is not associated with PCa risk in this material. The increased PCa risk may be attributed to increased surveillance and PSA screening.

  2. Risk of Prostate Cancer after Trans Urethral Resection of BPH: A Cohort and Nested Case-Control Study.

    Science.gov (United States)

    Karlsson, Camilla T; Wiklund, Fredrik; Grönberg, Henrik; Bergh, Anders; Melin, Beatrice

    2011-11-08

    Epidemiological and experimental evidence suggests that inflammation plays a role in both prostate cancer (PCa) and benign prostate hyperplasia (BPH). This study evaluates the risk of PC after transurethral resection (TURP) for BPH and estimates the PCa risk related to presence of inflammation in the resected material. The Pathology Department at the University Hospital of Umeå (Umeå, Sweden) identified BPH cases (n = 7,901) that underwent TURP between 1982 and 1997. Using these pathological specimens, we compared the incidence of PCa in the cohort to the population and calculated the standardized incidence and mortality ratios (SIR and SMR). Inflammation, the androgen receptor (AR), and p53 were evaluated in a nested case-control study of 201 cases and controls. Inflammation was graded severe or mild-moderate. In the follow-up period after TURP, cases developed prostate cancer and the controls did not. After TURP, SIR for prostate cancer increased [1.26, CI 95% (1.17-1.35)], whereas SMR decreased [0.59, CI 95% (0.47-0.73)]. Presence of inflammation at the time of TURP did not differ between cases and controls nor were there differences in p53 or AR staining. The data suggest a small increased risk of PCa after TURP and decreased PCa mortality. Inflammation at the time of TURP is not associated with PCa risk in this material. The increased PCa risk may be attributed to increased surveillance and PSA screening.

  3. Risk of Prostate Cancer after Trans Urethral Resection of BPH: A Cohort and Nested Case-Control Study

    Directory of Open Access Journals (Sweden)

    Beatrice Melin

    2011-11-01

    Full Text Available Epidemiological and experimental evidence suggests that inflammation plays a role in both prostate cancer (PCa and benign prostate hyperplasia (BPH. This study evaluates the risk of PC after transurethral resection (TURP for BPH and estimates the PCa risk related to presence of inflammation in the resected material. The Pathology Department at the University Hospital of Umeå (Umeå, Sweden identified BPH cases (n = 7,901 that underwent TURP between 1982 and 1997. Using these pathological specimens, we compared the incidence of PCa in the cohort to the population and calculated the standardized incidence and mortality ratios (SIR and SMR. Inflammation, the androgen receptor (AR, and p53 were evaluated in a nested case-control study of 201 cases and controls. Inflammation was graded severe or mild-moderate. In the follow-up period after TURP, cases developed prostate cancer and the controls did not. After TURP, SIR for prostate cancer increased [1.26, CI 95% (1.17–1.35], whereas SMR decreased [0.59, CI 95% (0.47–0.73]. Presence of inflammation at the time of TURP did not differ between cases and controls nor were there differences in p53 or AR staining. The data suggest a small increased risk of PCa after TURP and decreased PCa mortality. Inflammation at the time of TURP is not associated with PCa risk in this material. The increased PCa risk may be attributed to increased surveillance and PSA screening.

  4. Intraprostatische Phytoöstrogen-Gewebsspiegel in verschiedenen österreichischen Regionen im BPH- und Prostatakarzinomgewebe

    Directory of Open Access Journals (Sweden)

    Brössner C

    2006-01-01

    Full Text Available Einleitung: Zahlreiche Studien weisen darauf hin, daß die niedrige Inzidenz an Erkrankungen der Prostata (BPH und/oder Prostatakarzinom in asiatischen Ländern unter anderem am hohen Konsum an Phytoöstrogenen liegt. Weiters suggerieren jüngste Studien, daß das Prostatavolumen mit der Höhe der intraprostatischen Genisteinspiegel (= ein Phytoöstrogen negativ korreliert [1, 2]. Die intraprostatischen Genisteinspiegel sind bei asiatischen Männern im Vergleich zu österreichischen Männern um ein Vielfaches höher. Uns interessierte die Frage, ob analog zum Ost-West-Gefälle in unterschiedlichen geographischen Regionen Österreichs unterschiedlich hohe Gewebsspiegel nachweisbar sind. In einer Subgruppenanalyse evaluierten wir Unterschiede zwischen städtischem und ländlichem Umfeld. Material und Methodik: Prostatagewebechips von 103 österreichischen Männern von 3 verschiedenen Zentren (Graz, Salzburg, Oberwart wurden im Rahmen einer TUR-P oder radikalen Prostatektomie gewonnen und bei –40 °C gelagert. Davon wurden Enterolacton- (repräsentativ für die Gruppe der Lignane und Genisteinspiegel (repräsentativ für die Gruppe der Isoflavone mittels "monoclonal antibody based immunoassay" bestimmt und verglichen. Jede Messung wurde pro Patient doppelt durchgeführt. Ergebnisse: Die Enterolacton-Gewebsspiegel waren vergleichbar bei Männern, die in urbanem (median 19,1 ng/g dry weight; range 1,6–76,4 oder ruralem Umfeld lebten (median 15,7; range 0,6–140,6, p = 0,99. Ebenso vergleichbar waren für diese Gruppen die Werte für Genistein: 9,3 (range 0,1–156,7 und 20,5 (range 4,6–47,4, p = 0,77. Bei Männern, die in verschiedenen österreichischen geographischen Regionen lebten, waren ebenfalls keine Unterschiede zwischen den beiden Parametern Enterolacton und Genistein (p = 0,1 bzw. p = 0,65 zu finden. Schlußfolgerung: Generell sind innerhalb der untersuchten Gruppen die intraprostatischen Phytoöstrogenspiegel vergleichbar. Unter den

  5. Positive Response to Thermobalancing Therapy Enabled by Therapeutic Device in Men with Non-Malignant Prostate Diseases: BPH and Chronic Prostatitis

    Directory of Open Access Journals (Sweden)

    Ivan Gerasimovich Aghajanyan

    2016-04-01

    Full Text Available Background: The most common types of non-malignant prostate diseases are benign prostatic hyperplasia (BPH and chronic prostatitis (CP. The aim of this study was to find out whether thermobalancing therapy with a physiotherapeutic device is effective for BPH and CP. Methods: During a 2.5-year period, 124 men with BPH over the age of 55 were investigated. Clinical parameters were tested twice: via the International Prostate Symptom Score (IPSS and via ultrasound measurement of prostate volume (PV and uroflowmetry maximum flow rate (Qmax, before and after six months of therapy. In 45 men with CP under the age of 55, the dynamics of the National Institute of Health Chronic Prostatitis Symptom Index (NIH-CPSI were studied. Results: The results of the investigated index tests in men with BPH confirmed a decrease in IPSS (p < 0.001, a reduction in PV (p < 0.001, an increase in Qmax (p < 0.001, and an improvement of quality of life (QoL (p < 0.001. NIH-CPSI scores in men with CP indicated positive dynamics. Conclusions: The observed positive changes in IPSS, PV, and Qmax in men with BPH and the improvement in NIH-CPSI-QoL in patients with CP after using a physiotherapeutic device for six months as mono-therapy, support the view that thermobalancing therapy with the device can be recommended for these patients. Furthermore, the therapeutic device is free of side effects.

  6. Probing the molecular basis of substrate specificity, stereospecificity, and catalysis in the class II pyruvate aldolase, BphI.

    Science.gov (United States)

    Baker, Perrin; Carere, Jason; Seah, Stephen Y K

    2011-05-03

    BphI, a pyruvate-specific class II aldolase found in the polychlorinated biphenyls (PCBs) degradation pathway, catalyzes the reversible C-C bond cleavage of (4S)-hydroxy-2-oxoacids to form pyruvate and an aldehyde. Mutations were introduced into bphI to probe the contribution of active site residues to substrate recognition and catalysis. In contrast to the wild-type enzyme that has similar specificities for acetaldehyde and propionaldehyde, the L87A variant exhibited a 40-fold preference for propionaldehyde over acetaldehyde. The specificity constant of the L89A variant in the aldol addition reaction using pentaldehyde is increased ∼50-fold, making it more catalytically efficient for pentaldehyde utilization compared to the wild-type utilization of the natural substrate, acetaldehyde. Replacement of Tyr-290 with phenylalanine or serine resulted in a loss of stereochemical control as the variants were able to utilize substrates with both R and S configurations at C4 with similar kinetic parameters. Aldol cleavage and pyruvate α-proton exchange activity were undetectable in the R16A variant, supporting the role of Arg-16 in stabilizing a pyruvate enolate intermediate. The pH dependence of the enzyme is consistent with a single deprotonation by a catalytic base with pK(a) values of approximately 7. In H20A and H20S variants, pH profiles show the dependence of enzyme activity on hydroxide concentration. On the basis of these results, a catalytic mechanism is proposed.

  7. Shrinkage of Prostate and Improved Quality of Life: Management of BPH Patients with Croton membranaceus Ethanolic Root Extract

    Directory of Open Access Journals (Sweden)

    George Awuku Asare

    2015-01-01

    Full Text Available Benign prostatic hyperplasia (BPH is an enlargement of the prostate. The study aimed at validating the use of freeze-dried Croton membranaceus ethanolic root extract for BPH management. Thirty-three patients were observed before and after 3-month administration of 20 mg t.i.d orally. The International Prostate Symptom Score (IPSS, and the International Index of Erectile Function (IIEF questionnaires were used. Total/free PSA (tPSA, fPSA, renal, liver function, lipid tests, and ultrasonographic imaging were performed. Thirty (30 patients (66 ± 11 years completed the study. IPSS results showed 37% had severe, 40% moderate, and 23% mild symptoms before; 57% and 43% had moderate and mild symptoms, respectively, after treatment. IIED of patients’ results showed 30% with severe, 40% moderate, 24% mild-moderate, 3% mild, and 3% no erectile dysfunction before treatment and 20% severe, 43% moderate, and 37% mild-moderate dysfunction, after treatment. Quality of life (QoL improved (P=0.001. Significant but non-pathological increases in total and indirect bilirubin as well as apolipoprotein A occurred. Mean tPSA reduced from 27.9 ± 19.0 to 16.2 ± 11.8 ng/mL (P=0.002; fPSA from 6.1 ± 4.8 to 3.9 ± 2.9 ng/mL (P=0.045; and prostate volume from 101.8 ± 41.3 to 54.5 ± 24.8 cm3  (P=0.023. C. membranaceus shrinks the prostate and improves QoL.

  8. Two-micron (thulium) laser resection of the prostate-tangerine technique: a new method for BPH treatment

    Institute of Scientific and Technical Information of China (English)

    Shu-Jie Xia

    2009-01-01

    Two-micron (thulium) laser resection of the prostate-tangerine technique (TmLRP-TT) is a transurethral pro-cedure that uses a thulium laser fiber to dissect whole prostatic lobes off the surgical capsule, similar to peeling a tangerine. We recently reported the primary results. Here we introduce this procedure in detail. A 70-W, 2-μm (thulium) laser was used in continuous-wave mode. We joined the incision by making a transverse cut from the level of the verumontanum to the bladder neck, making the resection sufficiently deep to reach the surgical capsule, and resected the prostate into small pieces, just like peeling a tangerine. As we resected the prostate, the pieces were vaporized, sufficiently small to be evacuated through the resectoscope sheath, and the use of the mechanical tissue morcellator was not required. The excellent hemostasis of the thulium laser ensured the safety of TmLRP-TT. No patient required blood transfusion. Saline irrigation was used intraoperatively, and no case of transurethral resec-tion syndrome was observed. The bladder outlet obstruction had clearly resolved after catheter removal in all cases. We designed the tangerine technique and proved it to be the most suitable procedure for the use of thulium laser in the treatment of benign prostatic hyperplasia (BPH). This procedure, which takes less operative time than standard techniques, is safe and combines efficient cutting and rapid organic vaporization, thereby showing the great superi-ority of the thulium fiber laser in the treatment of BPH. It has been proven to be as safe and efficient as transurethral resection of the prostate (TURP) during the 1-year follow-up.April 2009.

  9. SYNTHESIS OF CATIONIC CERIUM COMPOUNDS [CP2CE(L)2][BPH4] (L = TETRAHYDROFURAN OR TETRAHYDROTHIOPHENE) AND THE CRYSTAL-STRUCTURE OF THE TETRAHYDROTHIOPHENE DERIVATIVE

    NARCIS (Netherlands)

    HEERES, HJ; MEETSMA, A; TEUBEN, JH

    1991-01-01

    Protolysis of the cerium alkyl Cp2*CeCH(SiMe3)2 (1) by triethylammoniumtetraphenylborate provides a useful route to cationic cerium compounds [Cp2*Ce(L)2][BPh4] (2, L = tetrahydrofuran; 3, L = tetrahydrothiophene). The crystal structure of the tetrahydrothiophene derivative was determined by X-ray d

  10. Comparison of trauma extent between transurethral plasmakinetic resection of the prostate and holmium laser enucleation treatment of BPH

    Institute of Scientific and Technical Information of China (English)

    Ying-Shu Zhen

    2016-01-01

    Objective:To study the trauma extent of transurethral plasmakinetic resection of the prostate and holmium laser enucleation treatment of BPH. Methods:A total of 134 cases of patients with benign prostatic hyperplasia were selected for study and randomly divided into PKRP group who received transurethral plasmakinetic resection of the prostate and HoLEP group who received transurethral holmium laser enucleation of the prostate, and serum prostate-specific antigen, cortin and medulla hormone as well as thyroid hormone content were detected. Results:1 d after operation, serum t-PSA and f-PSA content of both groups were higher than those before operation and serum t-PSA and f-PSA content of HoLEP group were lower than those of PKRP group, serum ACTH, COR, FC, CBG, NE, E and rT3 content of HoLEP group were significantly lower than those of PKRP group, FT3, TT3, FT4 and TT4 content were significantly higher than those of PKRP group, and TSH content was without significant difference;6 months after operation, serum t-PSA and f-PSA content of both groups were lower than those before operation and serum t-PSA and f-PSA content of HoLEP group were not different from those of PKRP group. Conclusion:Both transurethral plasmakinetic resection of the prostate and holmium laser enucleation can effectively remove hyperplastic prostate tissue, and the surgical trauma of HoLEP is less.

  11. Gambaran Histopatologi Penyakit Benign Prostatic Hyperplasia (BPH) dan Kanker Prostat di Laboratorium Patologi Anatomi Rumah Sakit Umum pusat Umum Pusat Haji Adam Malik Medan, periode 2008-2009

    OpenAIRE

    Hamawi, Mohd Lokman

    2010-01-01

    Background: Benign Prostatic Hyperplasia and prostate cancer are global heath problems, in either incidence occurrences or mortality. So, in order for early diagnosis and therapy, requires histopathologic features of BPH and prostate cancer. Location: Location of the study is at Rumah Sakit Umum Pusat Haji Adam Malik,Medan. It is choosen due to the number of cases available and to take account that this hospital is one of the centre of reference from parts of Sumatera Uta...

  12. Safety and efficacy of a novel Prunus domestica extract (Sitoprin, CR002) on testosterone-induced benign prostatic hyperplasia (BPH) in male Wistar rats.

    Science.gov (United States)

    Swaroop, Anand; Bagchi, Manashi; Kumar, Pawan; Preuss, Harry G; Bagchi, Debasis

    2015-01-01

    The efficacy of a novel Prunus domestica bark extract (Sitoprin, CR002) was investigated on testosterone propionate (TP)-induced benign prostatic hyperplasia (BPH) in male Wistar rats. BPH was induced by daily subcutaneous administration of TP (3.0 mg/kg) over a period of 15 days (interim sacrifice group) and for an additional 21 days (terminal sacrifice group). We evaluated the dose-dependent efficacy (0, 50, 100 and 200 mg/kg body weight/day) of CR002 and a control group against BPH, and compared with a reference standard Prunus africana extract (CR001). Extensive clinical examinations were carried out on days 1, 7, 14, 21, 28 and 35 of treatment period to determine the onset, duration and severity of clinical signs. Clinical pathology, hematology, biochemistry and histopathology were performed on days 15 and 35, prior to necropsy. Animals were fasted overnight prior to blood collection. Prostate glands and tissues were examined. On day 36, histopathology of ventral prostrate of control rats demonstrates single layer of columnar mucin secreting epithelial cells along with a lumen occupied with eosinophilic secretion. In contrast, CR002 and CR001 groups (100 and 200 mg/kg/day) exhibited no hyperplasia and proliferation of epithelial cells. Prostate histopathology of these treated groups was comparable with control rats. The hyperplasia and hypertrophy of prostrate was reduced to single-layered cell indicating the efficacy of CR002 and CR001. Overall, results demonstrate that CR002 exhibits therapeutic efficacy/activity in TP-induced BPH in rats, which is comparable to CR001.

  13. 前列腺液IL-6、IL-8在诊断BPH合并CP的临床意义%The clinical significance of IL-6 and IL-8 in EPS in diagnosis of BPH with chronic prostatitis

    Institute of Scientific and Technical Information of China (English)

    李兴斌; 王海峰; 常丛旺; 杨宇; 郭鹏飞; 赖建平

    2012-01-01

    Objective To investigate the significance that the concentration levels change of IL-6 and IL-8 in EPS were used to distinguish simple BPH and BPH with chronic prostatitis. Methods Patients with BPH who needed prostate surgery could through prostatic massage to get EPS before surgery, then the Concentration of IL-6 and IL-8 were measured. The prostate tissue specimens were Collected in surgery, according to the pathological prostatitis diagnosis standard to confirm research object of patients who had simple BPH and BPH with chronic prostatitis. Results The IL-6 and IL-8 in EPS which BPH with chronic prostatitis expressed was higher than simple BPH. Conclusion The Concentration levels of IL-6 and IL-8 in EPS which were close to patients whether BPH with chronic prostatitis were more effective to distinguish simple BPH and BPH with chronic prostatitis.%目的 探讨前列腺液(EPS)内IL-6(白细胞介素6)、IL-8浓度水平对区分单纯良性前列腺增生(BPH)和BPH合并慢性前列腺炎(CP)的意义.方法 100例前列腺手术的BPH病人,在术前行前列腺液IL-6、IL-8浓度检测.术中取前列腺组织标本,根据病理前列腺炎诊断标准确定单纯BPH患者与BPH合并CP患者.结果 BPH合并CP病人EPS中IL-6、L-8浓度较单纯BPH病人明显升高(P<0.01或P<0.05).结论 EPS中IL-6、IL-8浓度水平与BPH患者是否伴发CP的关系密切,二者在区别单纯BPH与BPH合并CP时具有较高价值.

  14. Benign Prostatic Hyperplasia (BPH)

    Science.gov (United States)

    ... of this procedure aren't available. Open or robot-assisted prostatectomy The surgeon makes an incision in ... transurethral microwave therapy. If you have open or robot-assisted prostatectomy, you might need to restrict activity ...

  15. Effects of obesity on lower urinary tract symptoms in Korean BPH patients%研究韩国人良性前列腺增生患者的肥胖症对下尿道综合症的影响

    Institute of Scientific and Technical Information of China (English)

    Seung Hwan Lee; Joon Chul Kim; Ji-Youl Lee; Jang Hwan Kim; Cheol Young Oh; Seung Wook Lee; Se Jeong Yoo; Byung Ha Chung

    2009-01-01

    We analyzed the effects of obesity on lower urinary tract symptoms (LUTSs) in Korean benign prostatic hyperplasia (BPH) patients. This is a multicenter, cross-sectional, prospective study conducted in four centers in Korea. A total of 602 men with LUTSs secondary to BPH were included. BPH/LUTSs cases were men aged ≥ 40 years with international prostate symptom scores (IPSS) ≥ 8 points. Height, weight and waist circumference were measured. Among the 602 patients, 156 patients had a waist circumference above 90 cm, representing central obesity, and 215 patients had a body mass index above 25 kg m~(-2). Waist circumference was positively correlated with prostate volume (P = 0.034). Men with waist circumference > 90 cm experienced a 1.36-fold increased risk of severe LUTSs (95% CI 0.82-2.41) compared with men with waist circumference ≤ 90 cm. Prostate volume was positively correlated with urgency and nocturia in men with central obesity. In this population of Korean men diagnosed with BPH, central obesity rather than overall obesity seems to be the more important predictor of LUTSs correlated with BPH.

  16. BPH combined with prostatitis:differences in clinical characters%良性前列腺增生合并前列腺炎患者的临床特点分析

    Institute of Scientific and Technical Information of China (English)

    李可; 杨金瑞

    2009-01-01

    目的 探讨合并前列腺炎的良性前列腺增生(BPH)患者的临床特点. 方法 BPH患者100例.按是否合并前列腺炎分为单纯组(34例)和合并组(66例).比较2组患者年龄、前列腺体积、PSA与前列腺特异性抗原密度(PSAD)、国际前列腺症状评分(IPSS)的差异,同时分析B超检查对BPH合并前列腺炎的检出情况. 结果单纯组患者年龄为(66.5±6.4)、合并组为(69.9±7.2)岁;单纯组平均前列腺体积为(47.5±26.7)、合并组为(71.4±39.3)ml.单纯组患者PSA和PS-AD中位数分别为3.40和0.08 ng/ml,合并组分别为8.07和0.12 ng/ml;平均IPSS分别为19.9和22.2.2组患者平均年龄、前列腺体积、PSA与PSAD值及IPSS评分比较,差异均有统计学意义(P<0.05).合并前列腺炎患者前列腺体积与炎症浸润程度及腺体破坏程度具有相关性(r分别为0.29,0.25,P<0.01).PSA与前列腺炎浸润分级和破坏分级均具有相关性(r分别为0.319和0.214,P值均<0.05).PSAD与浸润分级具有相关性(r=0.212,P<0.05).B超诊断BPH合并前列腺炎的敏感性为21.2%,特异性82.4%. 结论 BPH患者多伴前列腺炎,且以慢性炎性细胞浸润为主;合并前列腺炎的患者临床检测指标明显高于单纯BPH患者.B超对BPH合并前列腺炎的检出能力不令人满意.%Objective To analysis the differences of age, prostate volume, serum PSA and PSAD, IPSS between the BPH and BPH with prostatitis. Methods Clinical data of 100 patients whom received surgical treatments with a pathologic diagnosis of BPH were retrospectively analyzed. The occurrence of prostatitis was determined by pathology. The differences of age, prostate volume, serum PSA and PSAD, IPSS between the BPH cases and BPH with prostatitis cases were analyzed. The capability of B-ultrasound in diagnosis of the prostatitis combined with BPH was evaluated. Re-suits 66% BPH patients were found combined with prostatitis. There was a significant correlation between inflammatory infiltration

  17. BPH-1通过分泌PGE2上调前列腺间质细胞ERRα的表达%Prostaglandin E2 Mediates Up-regulation of ERRα Expression with BPH-1 Condition Medium in Prostatic Stromal Cells

    Institute of Scientific and Technical Information of China (English)

    苗琳; 石建党; 周颖; 杜小玲; 吴荃; 王克明; 张琚

    2008-01-01

    雌激素受体相关受体α(estrogen receptor-related receptor α,ERRα)是一类可以直接或间接参与雌激素应答反应的孤儿核受体,它与雌激素受体(estrogen receptor,ER)在结构上有很强的同源性.雌激素效应在良性前列腺增生(benign prostatic hyperplasis,BPH)的发生和发展中起着重要的作用.通常,孤儿核受体的转录活性多受一些非经典激素如维生素A衍生物、前列腺素类、固醇的调控.本文研究前列腺上皮细胞分泌的活性因子对间质细胞ERRα表达调控的分子机制.收集前列腺增生上皮细胞系BPH-1和前列腺癌上皮细胞系DU-145的条件培养液(condition medium,CM)培养间质细胞,采用实时定量RT-PCR和Western印迹法检测前列腺间质细胞(prostatic stromal cells,PrSC)中ERRα的表达,筛选CM中影响ERRα表达的活性因子.研究结果显示,BPH-1的CM可以上调ERRα的表达,而DU-145的CM对ERRα的表达没有影响;BPH-1中合成前列腺素E2(prostaglandin E2,PGE2)的限速酶--环氧合酶2(cyclooxygenase-2,COX-2)的mRNA表达水平和PGE2的分泌水平明显高于DU-145中COX-2表达水平和PGE2分泌水平;用经添加COX-2抑制剂NS-398的培养液处理BPH-1,其CM中PGE2的浓度明显下降,并失去了对ERRα表达的上调作用;添加PGE2可上调间质细胞中ERRα的表达.结果表明,BPH-1通过分泌PGE2促进间质细胞ERRα的表达,提示:在良性前列腺增生的发生和发展中,上皮细胞的旁分泌作用可促进间质细胞由ERRα介导的雌激素效应.

  18. Synthesis and Crystal Structure of Monochloridehepta (dimethyl sulfoxide)gadolinium (Ⅲ) di(tetraphenyl boron) Salt [Gd(dmso) 7Cl][BPh4]2

    Institute of Scientific and Technical Information of China (English)

    张道; 郭松山; 王汉章; 郁开北

    2000-01-01

    The crystal of the title compound (C62H82B2ClGdO7S7 Mr= 1378.02)is triclinic with space group P-1. a=12. 532(2), b=12. 774(2), c=24. 580(5)A; α=93.389(9), β=104. 640(8), γ=112. 923(9)°, V=3451.4(8)A3, Z=2, Dc=1.326g/cm3. μ(MoKα)=1. 258mm-1, F(000)=1426. R=0.036 , Rw= 0. 091for 12101 reflections with I>2σ(I). The crystal is composed of a discrete cation [Gd(dm-so )7Cl]+ and two anions [BPh4]-. The Gd( Ⅲ ) ion is coordinated by a chlorine and seven oxygen atoms from seven monodenate dimethylsulfoxides formimg a distorted square antiprism coordination polyhedron.

  19. Can finasteride reverse the progress of benign prostatic hyperplasia? A two-year placebo-controlled study. The Scandinavian BPH Study Group

    DEFF Research Database (Denmark)

    Andersen, J T; Ekman, P; Wolf, H;

    1995-01-01

    OBJECTIVES. To study if placebo-induced improvement in men with symptomatic benign prostatic hyperplasia (BPH) is maintained over 2 years, and to study the efficacy and safety from intervention with finasteride 5 mg for 24 months. METHODS. This was a multicenter, double-blind, placebo...... rate, prostate volume, postvoiding residual urinary volume, and serum concentrations of prostate-specific antigen together with laboratory safety parameters were measured at entry and at months 12 and 24. Interim physical and laboratory examinations were performed when indicated clinically. RESULTS....... The maximum urinary flow rate decreased in the placebo group, but improved in the finasteride group, resulting in a between-group difference of 1.8 mL/s at 24 months (P prostate volume was +12% in the placebo group versus -19% in the finasteride-treated group (P

  20. 利用BAC-ISH对抗性基因Bph3物理定位及水稻第四染色体着丝粒识别的研究%Physical Location of Resistance Gene, Bph3 and Identification of Chromosome 4 in Oryza sativa with BAC-ISH

    Institute of Scientific and Technical Information of China (English)

    覃瑞; 鄢彗民; 宋运淳

    2003-01-01

    用水稻BAC克隆21K19作探针进行了ISH.这个克隆是由第4染色体着丝粒连锁的RFLP标记RZ69筛选得到.在Cot-l DNA 封阻条件下,DAB均获得第4染色体着丝粒区和间期染色质上的一对信号.这一方法不仅可用于特定染色体识别,而且可区分其长臂和短臂.因此,还获得了第四染色体的臂比值以作为它的形态识别依据.由于RFLP标记RZ69与抗性基因Bph3紧密连锁,从而确定抗性基因Bph3位于水稻第4染色体着丝粒处.相对于常规的RFLP-FISH,BAC-ISH可以提高5倍以上的检出率.%Rice chromosome 4 was identified by ISH of centromere-linked BAC clone 21K19. This clone was screened by a single copy RFLP marker RZ69. It only showed a pair of signal spots on centromere regions of each member of one chromosome pair, i.e.,chromosome 4 as well as interphase chromatin under the blocking of rice Cot-1 DNA with DAB detection system. This is applicable not only for the recognizing of chromosome 4 but also for the distinguishing of its long and short arms. Therefore, we also obtained the arm ratio data of chromosome 4 for its morphological identification. From the molecular genetic map, we know that the RZ69 linked to resistance gene, Bph3, so in this study, resistance gene, Bph3 was physical located in centromere region of chromosome 4 in Oryza. sativa.Compared with the routine FISH of single copy RFLP marker can increase the detection rate more than five times.

  1. BPH中高度前列腺上皮内瘤的病理学观察%A Pathological Study of High Grade Prostatic Intraepithelial Neoplasia in BPH

    Institute of Scientific and Technical Information of China (English)

    蔡军; 卢慕峻; 赵雁飞

    2000-01-01

    目的了解良性前列腺增生症(BPH)病例中高度前列腺上皮内瘤(HGPIN)的发生率,并探讨HGPIN的病理基础及其与前列腺癌(PCa)之间的关系. 方法对54例临床诊断为BPH的病例进行病理学观察. 结果"54例BPH病例中有22例为HGPIN,占40.7%,平均年龄71.4岁.病理学特点为前列腺分泌层细胞明显增生,层次增多,核及核仁增大,核膜增厚,核仁清晰,有完整或不完整的基底细胞层,有完整的基底膜,并可见1例病理性核分裂象.腺体表现为四种形态,簇状、微乳头状、筛网状和平坦状. 结论 HGPIN在BPH中发生率较高,并且从病理形态学表现支持HGPIN为前列腺最可能的癌前病变.

  2. Field Calibration of the δ11B-pH Proxy in Corals and Calcified Algae at a Shallow Hydrothermal Vent and Adjacent Coral Reef

    Science.gov (United States)

    Day, R. D.; Christopher, S. J.; Young, C.; Brainard, R. E.; Butterfield, D. A.; Stewart, J.

    2015-12-01

    There is increasing interest in using the boron isotopic composition (δ11B) of biogenic carbonates as a proxy for seawater pH to better understand recent ocean acidification. The utility of this proxy hinges on the production of robust species-specific δ11B-pH calibrations; yet, challenges remain in the interpretation of boron isotope data due to biases introduced by physiological, environmental, and analytical factors. The shallow hydrothermal vents in the Maug Islands caldera (Marianas Islands) and the adjacent coral reefs exhibit a localized gradient > 1 pH unit. This gradient was used as a natural laboratory to assess the efficacy of using skeletal δ11B in a variety of corals (Porites spp., Pocillipora spp., Acropora spp.) and calcified algae (Halimeda spp. and Corallinales) as biosensors of seawater pH. Three sites were selected representing oceanic background, intermediate, and low pH zones, and direct seawater pH measurements were recorded for 3 months using SeaFETs. Corals and algae growing naturally in situ were collected from these 3 sites. In addition, corals and algae collected from a background location were stained and transplanted to these sites and allowed to grow for 3 months. Measurements of δ11B in skeletal material made by multi-collector inductively coupled mass spectrometry are compared to direct seawater pH measurements to assess the sensitivity and robustness of the δ11B proxy in these candidate biosensors in predicting ambient pH in the field. These data will inform ongoing efforts by the Archive of Coral Ecosystem Specimens (ACES) to collect marine carbonates for analysis and archival in the Marine Environmental Specimen Bank for broad-scale, long-term monitoring of ocean acidification and the associated impacts to coral reefs. Concurrent analyses of other trace elements, heavy metals, and isotopes in these samples will also be performed to assess their utility as biosensors for additional water chemistry parameters on coral reefs.

  3. Correlation between clinical prameter and histology of BPH%良性前列腺增生症组织形态学 及临床相关因素分析

    Institute of Scientific and Technical Information of China (English)

    李涛; 徐月敏; 金三宝; 王益鑫

    2001-01-01

    Purpose:Through analyzing the correlation between the parameter of the patient with benign prostate hyperplasia (BPH) and the histology of BPH,to explore the changeable cure of the BPH.Method:Hematoxylin and Eosin staining (HE)、Masson staining coupled with computer-assisted quantitative morphometric analysis was used to determine the hitological composition of 30 cases of BPH.Results:①The mean area densities of epithelium,glandular lumen,connective tissue and smooth muscle of BPH was 14.87±5.90%、22.54±6.99%、24.60±7.78% and37.98±9.33%.respectively.② The percentage area density of epithelium was bigger (P<0.01) and the percentage area density of smooth muscle was smaller (P<0.05) as the increasing of the weight of enucleated prostate adenoma.③ The percentage area density of smooth muscle was positive relation with the IPSS score of patients.(P=0.006).Conclusions:①Proliferation is mainly occurred in interstitial tissue,especially smooth muscle.②there is some kind of correlation between IPSS score and components of prostate tissue in BPH.This may benefit to the selection of clinical management.%目的:探讨良性前列腺增生症(BPH)临床参数与其组织学改变的关系,以指导临床治疗方法的选择。方法:应用HE染色和Masson染色结合计算机辅助图像分析的方法,对30 例BPH前列腺组织构成成分和5例正常前列腺组织进行了形态学的定量分析。结果:①BPH前列腺组织中上皮、腺腔、结缔组织和平滑肌的面积百分比分别是14.87%±5.90%、 22.54%±6.99%、24.60%±7.78%和37.98%±9.33%。间质∶上皮为(5.30±3.78)∶1, 平滑肌∶间质为(0.61±0.12)∶1。②随着腺体重量的增加,上皮面积百分比增加(P<0.01),平滑肌面积百分比下降 (P<0.05)。③BPH前列腺组织标本中的平滑肌面积百分比与IPSS 呈明显正相关(P<0.01)。结论:①BPH的前列腺以间质增生为主,且间质中的平滑肌增生更为突出。②BPH

  4. A multinuclear solid-state NMR study of alkali metal ions in tetraphenylborate salts, M[BPh4] (M = Na, K, Rb and Cs): what is the NMR signature of cation-pi interactions?

    Science.gov (United States)

    Wu, Gang; Terskikh, Victor

    2008-10-16

    We report a multinuclear solid-state ( (23)Na, (39)K, (87)Rb, (133)Cs) NMR study of tetraphenylborate salts, M[BPh 4] (M = Na, K, Rb, Cs). These compounds are isostructural in the solid state with the alkali metal ion surrounded by four phenyl groups resulting in strong cation-pi interactions. From analyses of solid-state NMR spectra obtained under stationary and magic-angle spinning (MAS) conditions at 11.75 and 21.15 T, we have obtained the quadrupole coupling constants, C Q, and the chemical shift tensor parameters for the alkali metal ions in these compounds. We found that the observed quadrupole coupling constant for M (+) in M[BPh 4] is determined by a combination of nuclear quadrupole moment, Sternheimer antishielding factor, and unit cell dimensions. On the basis of a comparison between computed paramagnetic and diamagnetic contributions to the total chemical shielding values for commonly found cation-ligand interactions, we conclude that cation-pi interactions give rise to significantly lower paramagnetic shielding contributions than other cation-ligand interactions. As a result, highly negative chemical shifts are expected to be the NMR signature for cations interacting exclusively with pi systems.

  5. “诊断性”TURP在BPH伴血清PSA异常患者中的应用价值%The Role of diagnostic TURP in patients with elevated serum PSA levels BPH

    Institute of Scientific and Technical Information of China (English)

    袁祖亮; 李文成; 贾瑞鹏; 许露伟; 徐郑; 梁凯; 吴剑平; 窦全亮; 朱佳庚

    2013-01-01

    目的:探讨“诊断性”TURP在BPH伴血清PSA异常患者中的应用价值及意义.方法:对BPH伴血清PSA异常患者行“诊断性”TURP,然后对手术切除的前列腺组织行病理检查,按照病理诊断结果分为PCa组和BPH组,分别评估PCa组患者的临床分期及Gleason评分和BPH组患者TURP术后3个月、6个月、1年及随访结束时血清PSA、IPSS评分、生活质量等.结果:87例BPH伴血清PSA异常者行“诊断性”TURP,病理诊断为PCa 5例,Gleason评分5~8分,肿瘤分期T1a~T2a,行PCa根治性切除术;病理诊断为BPH 82例,出院后均随访2~4年,其中血清PSA恢复正常者77例,显著下降者3例,持续异常者2例,IPSS评分均有明显降低.结论:对血清PSA异常患者,“诊断性”TURP可有助于诊断PCa并对下尿路症状的改善和血清PSA的正常化具重要作用.%Objective:To evaluate the value and significance of diagnostic transurethral resection of the prostate(TURP) in the patients with persistently abnormal serum PSA levels.Methods:BPH patients with elevated serum PSA levels (>4 μg/L) underwent TURP.According to pathological examination of prostate tissue removed in TURP,we divided the patients into the PCa group and BPH group.Gleason score,clinical stage were evaluated for PCa patients,serum PSA testing and the International Prostate Symptom Score (IPSS) etc were evaluated for BPH patients every three months,six months and one year respectively.Results:Of 87 patients,82 cases were diagnosed as BPH by pathological examination postoperatively,5 cases were PCa,Gleason score 5-8,tumor stage T1a-T2a and radical prostatectomy was done.Followed up 2-4 years,77 patients have normal serum PSA,3 serum PSA reduced greatly,2 persistently abnormal serum PSA,IPSS for all patients reduced greatly.Conclusion:Diagnostic TURP can be used as an important method in the diagnosis of patients with elevated serum PSA levels.Also the lower urinary tract symptoms could be relieved by TURP

  6. Pyramiding the Blast Resistant Gene Pi9 and the Brown Planthopper Gene Bph18(t) to Develop Restorer Lines in Rice (Oryza sativa L.)%聚合抗稻瘟病基因Pi9和抗褐飞虱基因Bp h18(t)选育水稻恢复系

    Institute of Scientific and Technical Information of China (English)

    马文清; 裴庆利; 梁云涛; 刘丕庆; 赵开军; 王春连; 林纬; 杨培忠; 于洁

    2014-01-01

    水稻(Oryza sativa L.)作为粮食作物,在全世界粮食生产中具有极其重要的地位。稻瘟病、褐飞虱等病虫害的发生严重危害着水稻的安全生产,培育并利用水稻抗性品种能经济有效地预防水稻病虫害的发生,因此,培育抗性品种在水稻安全生产中尤为重要。本研究以具有抗稻瘟病基因Pi9的抗病品系75-1-127为抗病亲本,以携带有抗褐飞虱基因Bph18(t)的水稻材料C4064为抗虫亲本,以性状优良的恢复系测679作为轮回亲本,进行杂交、回交和自交,并辅以田间多代选择。在分离群体中,使用与Pi9紧密连锁的SCAR标记pB8跟踪目标基因Pi9,利用与Bph18(t)紧密连锁的标记KC16跟踪目标基因Bph18(t)。通过分子标记辅助选择、农艺性状评价和抗病抗虫鉴定,选育出聚合了Pi9和Bph18(t)基因、对稻瘟病和褐飞虱的抗性水平接近抗病亲本或抗虫亲本的恢复系。%Rice (Oryza sativa L.), a cereal crop, plays an ultimately important role in the global food production. The occurrence of the rice disease and pest such as the rice blast (RB) and the brown planthopper (BPH) can seve-rely damage the safe production of rice, and the development and application of the resistant varieties can prevent the occurrence of the rice disease and pest economically and effectively, so the development of the resistant variety is crucial in the safe production of rice. In this study, we used the disease resistant line 75-1-127 carrying rice blast resistant gene Pi9 as the disease-resistant parent, the BPH-resistant line C4064 harboring brown planthopper resistant gene Bph18(t) as the BPH-resistant parent, and the elite restoring line Ce679 as recurrent parents, to hy-bridize, backcross, selfcross and field select for multiple generations. The Pi9-linked SCAR marker pB8 was used to select Pi9, and the Bph18(t)-linked marker KC16 was used to select Bph18(t) in the segregating populations. By

  7. A phytosterol enriched refined extract of Brassica campestris L. pollen significantly improves benign prostatic hyperplasia (BPH) in a rat model as compared to the classical TCM pollen preparation Qianlie Kang Pule'an Tablets.

    Science.gov (United States)

    Wang, Ruwei; Kobayashi, Yuta; Lin, Yu; Rauwald, Hans Wilhelm; Fang, Ling; Qiao, Hongxiang; Kuchta, Kenny

    2015-01-15

    In Qinghai Province, the Brassica campestris L. pollen preparation Qianlie Kang Pule'an Tablet (QKPT) is traditionally used for BPH therapy. However, in QKPT the content of supposedly active phytosterols is relatively low at 2.59%, necessitating high doses for successful therapy. Therefore, a phytosterol enriched (4.54%) refined extract of B. campestris pollen (PE) was developed and compared with QKPT in a BPH rat model. Six groups of rats (n=8 each), namely sham-operated distilled water control, castrated distilled water control, castrated QKPT 2.0g/kg, castrated PE 0.1g/kg, castrated PE 0.2g/kg, and castrated PE 0.4g/kg, were intragastrically treated with the respective daily doses. Testosterone propionate (0.3mg/day) was administered to all castrated rats, while the sham-operated group received placebo injections. After 30 days, the animals were sacrificed and prostates as well as seminal vesicles excised and weighted in order to calculate prostate volume index (PVI) as well as prostate index (PI) and seminal vesicle index (SVI), defined as organ weight in g per 100g body weight. Compared with sham-operated controls, PI (p<0.01), PVI (p<0.01), and SVI (p<0.01) were all significantly increased in all castrated, testosterone treated rats. After treatment with PE at 0.4 and 0.2g/kg or QKPT at 2.0g/kg per day, both indices were significantly reduced (p<0.01) as compared to the castrated distilled water control. For PE at 0.1g/kg per day only PI was significantly reduced (p<0.05). At the highest PE concentration of 0.4g/kg per day both PI and SVI were also significantly reduced when compared to the QKPT group (p<0.05). Both PE and QKPT demonstrated curative effects against BPH in the applied animal model. In its highest dose at 0.4g/kg per day, PE was clearly superior to QKPT.

  8. Transurethral Electrotherapy: Treatment of 211 Cases of Benign Prostatic Hyperplasia%经尿道电气化术治疗前列腺增生症(BPH)-附211例报告

    Institute of Scientific and Technical Information of China (English)

    金一笑; 郑军政; 丁光海

    2003-01-01

    目的:讨论经尿道电气化治疗良性前列腺增生症.方法:采用经尿道电气化术(TUVP)治疗BPH211例,随访5~15m.结果:术后国际前列腺症状评分(IPSS)平均7.5分,最大尿流率平均17.4ml/S,残余尿量28ml.结论:经尿道前列腺电气化术是一种安全、有效、并发症少的手术方法.

  9. Simultaneous tension-free hernioplasty and transurethral resection of prostate for the treatment of BPH complicated with inguinal herniahe%18F-FDG PET-CT在胃癌术前诊断中的临床应用研究

    Institute of Scientific and Technical Information of China (English)

    王瑜

    2011-01-01

    目的 探讨18F-FDG PET-CT在胃癌术前诊断中的应用价值.方法 筛选该院初诊胃癌患者55例对其进行CT、PET和PET-CT检查,与术后的病理学检查比较,分析诊断结果.结果 恶性胃癌的SUVmas平均值明显高于良性胃癌,P<0.05;PET-CT在灵敏度上高于CT和PET,在阳性检测率上三者没有区别,P>0.05;PET-CT与术后病理检查的符合性较好,P<0.05.结论 18F-FDG PET-CT在胃癌术前诊断具有较大的诊断价值.%Aim To investigate the surgical effects of transurethral resection of prostate in combination with tension-free hemioplasty in patients with benign prostate hyperplasia(BPH) and inguinal hernia. Methods The clinical material carries on the review analysis that 38 cases of BPH complicated with inguinal hernia were treated with TURP and one stage tension-free repair of inguinal hernia from July 2005 to February 2011. In this group 38 cases,with an the average age of 69 (55 ~ 86) year old, were slanting hernia, 3 recurrence slanting hernia, merge the hypertension 7,2 Diabetes 3, chronic bronchitis 3. Results They all went through surgical operation smoothly, with tension-free Hernioplasty time of 30 ~95 min, average 45 min, transurethral Resection of Prostate time 35 ~ 125 min, average 55 min, the excision prostate gland weight 15 ~ 60 g, average 25 g, bleed measure a 40 ~ 300 ml, average 90 ml. Nobody had blood tranfusion. Conclusion Transurethral resection of prostate in combination with tension-free hernioplasty has a satisfied effect for benign prostate hyperplasia with inguinal hernia, has avoided second-time surgery, has the advantages of small damage and few complications.

  10. 移行带前列腺癌与良性前列腺增生患者磁共振波谱的检测%Differentiation of prostate cancer from benign prostatic hyperplasia (BPH) in the transitional zone by magnetic resonance spectroscopic imaging

    Institute of Scientific and Technical Information of China (English)

    李飒英; 陈敏; 王蕊; 王文超; 赵伟峰; 周诚

    2010-01-01

    对2007年7月至2009年12月收治的20例移行带前列腺癌和20例良性前列腺增生(BPH)患者的磁共振波谱成像(MRSI)资料进行回顾性分析.移行带癌与增生活检组织体素的胆碱和肌酸与枸橼酸盐比值(CC/Ci)分别为2.26±1.35与0.81±0.22,胆碱与肌酸比值(Cho/Cr)分别为4.12±1.65与1.25±0.35,两者比较差异有统计学意义(t=5.17,t=7.16,均P<0.01);此二比值鉴别前列腺癌与BPH的特异性、敏感性和准确性分别为99%、86%和93%,有重要的临床价值.%Twenty patients with prostate cancer and 20 patients with benign prostate hyperplasia (BPH) in the transitional zone underwent magnetic resonance imaging (MRI) /magnetic resonance spectroscopic imaging ( MRSI ) examinations. The choline + creatine/citrate (CC/Ci) ratio and the choline/creatine (Cho/Cr) ratio were evaluated in each voxel with cancer or BPH confirmed by pathological results.Discriminant analysis was used to determine the power of the two ratios in differentiation between cancer and BPH. The CC/Ci ratio and Cho/Cr ratio for cancer voxels were significantly higher than those in the voxels with BPH in the transitional zone (CC/Ci: 2.26 ± 1.35 vs. 0.81 ±0.22, t =5.17, P <0. 01, Cho/Cr:4. 12 ± 1.65 vs. 1.25 ±0. 35, P<0. 01 ). As for the discriminant function with the CC/Cr ratio and the Cho/Cr ratio, the specificity, sensitivity, and accuracy were 99%, 86%, 93% respectively for the differentiation between cancer and BPH.

  11. Synthesis, spectroscopic and redox properties of the mononuclear NiII, NiII(BPh2)2 containing (B-C) bond and trinuclear CuII-NiII-CuII type-metal complexes of ,'-(4-amino-1-benzyl piperidine)-glyoxime

    Indian Academy of Sciences (India)

    Ahmet Kilic; Esref Tas; Ismail Yilmaz

    2009-01-01

    The novel vic-dioxime ligand containing the 4-amino-1-benzyl piperidine group, ,'-(4-amino-1-benzyl piperidine)-glyoxime, (LH2) has been prepared from 4-amino-1-benzyl piperidine with anti-dichloroglyoxime at -15°C in absolute THF. Mononuclear NiII metal complex has been obtained with 1 : 2 metal/ligand ratio. The NiII complex of this ligand is proposed to be square planar geometry. IR spectra show that the ligand acts in a tetradentate manner and coordinates N4 donor groups of LH2 to NiII ion. The detection of H-bonding (O-H$\\cdots$O) in the [Ni(LH))2] (${\\rm 1}$) metal complex by IR spectra supported the square-planar MN4 coordination of mononuclear complex. The disappereance of H-bonding (O-H$\\cdots$O) in the [Ni(L)2(BPh2)2] (2) complex shows that the BPh$^{+}_{2}$-capped groups (BPh$^{+}_{2}$ cation formed BPh4 anion) attaches to the main oxime core. MN4 coordination of the [Ni(LH)2] (1) and [Ni(L)2(BPh2)2] (2) metal complexes were also determined by 1H-NMR spectroscopy. In the trinuclear CuII-NiII-CuII metal complexes, the NiII ion centered into the main oxime core by the coordination of the imino groups while the two CuII ions coordinate dianionic oxygen donors of the oxime groups and linked to the ligands of 1,10-phenanthroline, 2,2'-bipyridine, and 4,4'-bipyridine. The ligand and their mono and trinuclear metal complexes were characterized by elemental analyses, FT-IR, UV-Vis, 1H and 13C-NMR spectra, magnetic susceptibility measurements, molar conductivity, cyclic voltammetry, mass spectra and X-ray powder techniques. The cyclic voltammetric results show that the cathodic peak potential of [Ni(L)2(BPh2)2] shifted toward more negative value compared to that of [Ni(LH)2], probably due to a decreasing effect of back donation of metal-oxime moieties as a result of the BPh$^{+}_{2}$-bridged complex formation. Also, the formation of the trinuclear CuII-NiII-CuII metal complexes caused considerable changes on the CV behaviour of mononuclear [Ni(LH)2] (1

  12. 前列腺增生症合并膀胱过度活动症相关因素研究%Study of BPH With Coexisting Overactive Bladder Dysfunction

    Institute of Scientific and Technical Information of China (English)

    王国勇

    2013-01-01

    Objective: The aim of this study was to use a systematic schedule ,including urodynamics.to describe the rate of coexisting overactive bladder(OAB) in patients with baldder outlet obstruction(BOO) caused by benign prostatic hyperplasis(BPH).We also identified differences between the paticents with pure BOO compared with BOO combined with OAB(BOO+OAB). Methods:One hundered and five men referred to our clinic due to LUTS were included. Patients with a history that might affect their bladder function were excluded . After cystometry and pressure-flow studies,the patients were divided into pure BOO and BOO+OAB. Of the 79 men .Results:64.6% had pure BOO.BOO+OAB was found in 35.4%. PSA,IPSS, volume ,voided volume , PVR , obstruction grade and OABSS differed significantly between the groups .The patients with BOO+OAB had a higher PSA,IPSS, volume ,OABSS, voided smaller volumes, PVR were smaller and were more obstructed .We found no differences in Age ,Cr or Q-max .There was a strong association between OAB and BOO, the percentage of OAB increasing with increased obstruction. Age ,Cr and Q-max did not predict whether the patients had a combined BOO+OAB or not . Conclusions:These finding indicate that BOO is a progressive disease , which in time causes pronounced obstruction and perhaps in itself contributes to the development of OAB.%目的:系统的通过尿流动力学检查,明确良性前列腺增生症合并膀胱过度活动症的相关因素,以利临床医师明确区分 BPH 的 LUTS症状是单纯 BOO 或 BOO+OAB,以具体病情制定进一步治疗.方法:105例有明确 LUTS 的 BPH 的患者.有影响膀胱功能的其他疾病除外.第一步均进行常规的病史、体格检查和 DRE ,IPSS、OABSS、年龄、血清肌酐、PSA、尿液的常规检查和培养等、B 超测定前列腺体积,第二部行尿流率检查:记录 Qmax、VV、PVR(残余尿量,导尿法);第三步:行尿流动力学膀胱充盈测压和压力-流率测定.第四步:

  13. Prostate Enlargement: Benign Prostatic Hyperplasia (BPH)

    Science.gov (United States)

    ... not they have urinary problems. Digital rectal exam Medical Tests A health care provider may refer men to a urologist—a doctor ... the following side effects should contact a health care provider right away or get emergency medical care: hives rash itching shortness of breath rapid, ...

  14. LUTS in BPH patients with histological prostatitis before and after transurethral resection of the prostate%合并组织学前列腺炎的良性前列腺增生患者TURP手术对下尿路症状的影响

    Institute of Scientific and Technical Information of China (English)

    黄向华; 关挺; 覃斌; 梁毅文; 吴清国; 李长赞; 韦钢山; 季汉初; 梁阳冰; 陈红秋

    2013-01-01

    Objective:To investigate the effects of transurethral resection of the prostate (TURP) on lower urinary tract symptoms (LUTS) in patients with benign prostatic hyperplasia (BPH) complicated by histological prostatitis. Methods:This study included 432 cases of BPH pathologically confirmed after TURP. Excluding those with LUTS-related factors before and after surgery and based on the international prostatitis histological classification of diagnostic criteria, the remaining 144 cases were divided into groups A ( pure BPH, n = 30) , B ( mild inflammation, n = 55) , C ( moderate inflammation, n = 31) , and D ( severe inflammation, n = 28 ) . Each group was evaluated for LUTS by IPSS before and a month after surgery. Results: A total of 399 cases (92.4% ) were diagnosed as BPH with histological prostatitis, 269 (67.4% ) mild, 86 (21. 6% ) moderate and 44 ( 11. 0% ) severe. The preoperative IPSS was 21.43 ±6.09 in group A, 21.75 ±5.97 in B, 27. 84 ±4.18 in C and 31.00 ±2.92 in D, with statistically significant differences among different groups (P<0. 001) except between A and B (P = 1.000); the postoperative IPSS was 5. 60 ±2. 16 in A, 7. 36 ±2.77 in B, 11.55 ±3. 39 in C and 16. 89 ± 3. 37 in D, with statistically significant differences among different groups (P < 0.01) , and remarkably lower than the preoperative one (P<0.001). Almost all the infiltrating inflammatory cells in BPH with histological prostatitis were lymphocytes. Conclusion; BPH is mostly complicated with histological chronic prostatitis. The severity of LUTS is higher in BPH patients with histological prostatitis than in those without before and after TURP, and positively correlated with the grade of inflammation. Those complicated with moderate or severe histological prostatitis should take medication for the management of LUTS.%目的:探讨合并组织学前列腺炎的良性前列腺增生(BPH)患者行经尿道前列腺电切术(TURP)对下尿路症状的影响. 方法:对2009年5月至2011

  15. 中老年男性人群代谢综合征和良性前列腺增生的相关性分析%Analysis of the Correlation Between Metabolic Syndrome and BPH in Middle and Old-aged Men

    Institute of Scientific and Technical Information of China (English)

    朱波; 李勐

    2014-01-01

    Objective] To explore the correlation between metabolic syndrome and benign prostatic hyperplasia (BPH) in middle and old‐aged men .[Methods] Totally 151 middle and old‐aged men(≥50 years old) in physical exami‐nation center of our hospital from May 2008 to July 2013 were chosen .The general examinations included age ,height , weight ,blood pressure ,past history and IPSS score .The laboratory tests included fasting blood glucose ,blood lipids and PSA .The image test was transrectal ultrasound for measuring prostate volume .Urine flow rate(QMax) was measured . The incidence of BPH between metabolic syndrome and non‐metabolic syndrome was compared .The correlation of factors in metabolic syndrome with BPH was analyzed .[Results]According to examination results ,all cases were divided into metabolic syndrome group( n =37) and non‐metabolic syndrome group( n =114) .The incidence of BPH in metabolic syndrome group and non‐metabolic syndrome was 32 .4% (12/37) and 12 .3% (14/114) ,respectively .There was signifi‐cant difference in IPSS ,prostate volume and PSA between two groups( P<0 .05) .There was no significant difference in QMax between two groups( P=0 .071) .Obesity ,hyperlipidemia and diabetes mellitus were the risk factors of BPH(OR=1 .72 ,3 .31 ,2 .07 ,P=0 .042 ,0 .029 ,0 .046) .[Conclusion]The morbidity of BPH in patients with metabolic syn‐drome is higher .IPSS ,prostate volume and PSA are the main influential factors of BPH patients .Obesity ,hyperlipi‐demia and diabetes are the risk factors of BPH .%目的 探讨中老年男性人群代谢综合征与良性前列腺增生(BPH)的相关性。方法 选择2008年5月至2013年7月于本院体检中心体检的中老年男性(≥50岁)共151例,检查一般项目包括年龄、身高、体质量、血压、既往史、国际前列腺症状评分表(IPSS)评分等。实验室检查包括空腹血糖、血脂、前列腺特异性抗原(PSA)等。影像学检查包括经直

  16. Effects of Danggui-beimu-kushen Pill on expression of PCNA, bcl-2 and bax in BPH mice model%当归贝母苦参丸对良性前列腺增生模型小鼠PCNA、bcl-2和bax表达的影响

    Institute of Scientific and Technical Information of China (English)

    张奇峰; 黄宗轩; 高国政; 贺铁豪

    2011-01-01

    Objective To study the effect of Danggui-beimu-kushen Pill on benign prostatic hyperplasia (BPH) and its possible mechanism. Methods BPH model mouse was produced by intraperitoneal injection of testosterone propionate. The high, medium and low dose treatment group was fed high, middle and low dose of Danggui-beimu-kushen Pill respectively, while the positive control group was fed Qianliekang liquid, 600 mg/kg. All mice were executed on the 21 day. Such values were observed as the prostate index changes, pathological changes of prostate by HE staining light microscopy and the expression changes of PCNA,bcl-2 and bax by immunohistochemistry. Results The prostate index of the treatment group was lower than the model group; the PCNA and bcl-2 expression were lower than the model group, while the bax expression was higher than the model group. Meanwhile; there is no significant difference among the treatment groups.Moreover, the difference between the treatment groups and the positive control group has no statistic meaning.Conclusion Danggui-beimu-kushen Pill can suppress BPH in mice. The mechanism may be related to the reduction expression of cell-proliferation protein PCNA and apoptosis profilin bcl-2, and the increase of bax expression.%目的 探讨当归贝母苦参丸对良性前列腺增生(BPH)模型小鼠的影响及作用机理.方法 将60只雄性小鼠,按照随机数字表法随机分为6组:空白组,模型组,阳性对照组,高、中、低剂量治疗组.空白组给予腹腔注射12.5 mg/kg·d-1橄榄油,其他各组采用腹腔注射内酸睾酮原液制作小鼠BPH模型,同时高、中、低剂量治疗组分别予高、中、低剂量当归贝母苦参丸水煎溶液灌胃,阳性对照组灌胃前列康水混悬液600 mg/kg.给药第21天处死,观察前列腺指数(PI)的变化;HE染色光镜观察前列腺病理改变;免疫组化方法检测PCNA、bcl-2和bax表达的变化.结果 当归贝母苦参丸治疗组PI低于模型组;PCNA、bcl-2表

  17. Bcl-w和PDCD5在前列腺增生和前列腺癌组织细胞中的表达及相关性%Expressions of bcl-w and PDCD5 in tissues of BPH and prostate cancer

    Institute of Scientific and Technical Information of China (English)

    高庆强; 陈仁富; 朱海涛; 孙晓磊; 胡亮亮; 孙晓青

    2014-01-01

    目的 研究凋亡相关基因Bcl-w和PDCD5在前列腺癌(PCa)、良性前列腺增生(BPH)和正常前列腺(NP)组织中表达的相关性及其意义.方法 通过免疫组织化学法检测10例NP、25例BPH及25例PCa组织切片Bcl-w和PDCD5的表达.结果 NP组、BPH组、PCa组Bcl-w染色阳性率分别为20%、36%、64%;NP组,BPH组,PCa组PDCD5染色阳性率分别为80%、68%、28%.将Bcl-w蛋白和PDCD5蛋白在不同病例组织中的染色强度指数(staining intensity index,SII)做进一步的Pearson直线相关性分析,提示在NP、BPH以及PCa癌组织中,Bcl-w SII和PDCD5 SII呈负相关(r=-0.904,P<0.05).结论 不论Bcl-w的过度高表达,还是PDCD5的低表达都与前列腺癌的发病相关,Bcl-w和PDCD5在前列腺癌的发生与发展中可能互相影响.这两项指标可作为辅助检测前列腺癌变的基因标志物.%Objectives To study the relationship of the expressions of apoptosis-related gene Bcl-w and PDCD5 in tissues of prostate cancer(PCa),BPH and normal human prostate(NP).Methods The expressions of Bcl-w and PDCD5 were observed separately in 10 normal human prostate(NP),25 of BPH,and 25 of prostate cancer(PCa) by Immunohistochemical means.Results Bcl-w positive rate in normal human prostate(NP) was 20%,in BPH was 36%,and in prostate cancer(PCa) was 64%.PDCD5 positive rate in normal human prostate (NP) was 80%,in BPH was68%,and in prostate cancer(PCa) was 28%.There was a negative relationship between Bcl-w SII and PDCD5 SII in every lesion.Conclusions It suggests that both Bcl-w and PDCD5 could be used as molecular markers of carcinogenesis for prostate cancer(PCa).

  18. News Girls in Physics: Getting girls engaged with physics Schools Lecture: How to explore the universe: the IOP schools lecture series 2009 Elastomobile Competition: Rubber-band vehicles go for gold Congress: Congress celebrates centenary Outreach Programme: Tales of the Stars inspires young children from around the world Physics Olympiad: BPhO selects top students for International Physics Olympiad Mobile Science: Mobile teaching lab visits rural Turkey China: Inspiration and competition in China

    Science.gov (United States)

    2009-07-01

    Girls in Physics: Getting girls engaged with physics Schools Lecture: How to explore the universe: the IOP schools lecture series 2009 Elastomobile Competition: Rubber-band vehicles go for gold Congress: Congress celebrates centenary Outreach Programme: Tales of the Stars inspires young children from around the world Physics Olympiad: BPhO selects top students for International Physics Olympiad Mobile Science: Mobile teaching lab visits rural Turkey China: Inspiration and competition in China

  19. Simultaneous transurethral resection of bladder tumor and prostate for bladder carcinoma patients complicated with BPH%同期经尿道电切术治疗膀胱癌合并良性前列腺增生的临床分析

    Institute of Scientific and Technical Information of China (English)

    李碧君; 单炽昌; 王在盛; 叶亲永

    2011-01-01

    目的 探讨同期经尿道切除膀胱肿瘤和前列腺治疗表浅性膀胱癌合并良性前列腺增生症的手术安全性和临床疗效.方法 72例表浅性膀胱癌合并良性前列腺增生症患者,先行经尿道膀胱肿瘤电切术(TURBT)切除肿瘤后同期行经尿道前列腺电切术(TURP)切除前列腺.结果 患者均顺利完成手术,无膀胱穿孔和电切综合征发生,术后随访14~54个月,平均24个月,35例发生膀胱肿瘤复发,平均复发时间16个月,复发部位均不在膀胱颈口和前列腺尿道,全部再次行TURBT.结论 同期经尿道切除膀胱肿瘤和前列腺治疗表浅性膀胱癌合并良性前列腺增生症手术安全、短期疗效确切,可适用于一部分年龄较大伴有严重的下尿路梗阻的且肿瘤分期、分级低的表浅性膀胱肿瘤患者.%Objective To evaluate the feasibility and clinical efficacy of simultaneous transurethral resection of bladder tumor (TURBT) and transurethral resection of prostate (TURP) in treatment of superficial bladder cancer(SBC) with benign prostatic hyperplasia(BPH).Methods The clinical data of 72 patients with SBC and BPH were retrospectively reviewed.All the patients received simultaneous TURBT and TURP.Results All patients were successfully operated, no bladder perforation and transurethral resection syndrome occurred.The follow-up period ranged from 14-54months.Recurrence of bladder cancer occurred in 35 patients after average of 16months, but no bladder neck and prostatic urethra implantation.All of them were treated with TURBT again.Conclusions Our data indicate that simultaneous TURBT and TURP is a safe and effective treatment for old SBC patients with BPH, and is applicable to some old patients with lowstage and low-grade superficial bladder tumors complicated with severe lower urinary tract obstruction.

  20. Sequence of the bphD gene encoding 2-hydroxy-6-oxo-(phenyl/chlorophenyl)hexa-2,4-dienoic acid (HOP/cPDA) hydrolase involved in the biphenyl/polychlorinated biphenyl degradation pathway in Comamonas testosteroni: evidence suggesting involvement of Ser112 in catalytic activity.

    Science.gov (United States)

    Ahmad, D; Fraser, J; Sylvestre, M; Larose, A; Khan, A; Bergeron, J; Juteau, J M; Sondossi, M

    1995-04-14

    The nucleotide sequence of bphD, encoding 2-hydroxy-6-oxo-(phenyl/chlorophenyl)hexa-2,4-dienoic acid hydrolase involved in the biphenyl/polychlorinated biphenyl degradation pathway of Comamonas testosteroni strain B-356, was determined. Comparison of the deduced amino-acid sequence with published sequences led to the identification of a 'lipase box', containing a consensus pentapeptide sequence GlyXaaSerXaaGly. This suggested that the mechanism of action of this enzyme may involve an Asp-Ser-His catalytic triad similar to that of classical lipases and serine hydrolases. Further biochemical and genetic evidence for the active-site involvement of Ser112 was obtained by showing that a semipurified enzyme was inhibited by PMSF, a classic inhibitor of serine hydrolases, and by site-directed Ser112-->Ala mutagenesis.

  1. 特拉唑嗪治疗良性前列腺增生合并原发性高血压的临床研究%Efficacy of terazosin in the treatment of the patients with benign prostatic hyperpla-sia (BPH) complicated with hypertension

    Institute of Scientific and Technical Information of China (English)

    庄红雨; 彭涛; 许学敏

    2014-01-01

    Objective To observe and evaluate the efficacy,safety and compliance of terazosin in the treat-ment of the patients with benign prostatic hyperplasia ( BPH) complicated with hypertension. Methods 200 cases of patients with BPH complicated with hypertension were selected and randomly divided into observation group and con-trol group,100 cases in each group. Patients in control group were treated with normal antihypertensive drug,while the patients in observation group were added oral administration of terazosin on the basis of the therapy of control group. The patients were followed up for 3 months. The changes of international prostate symptom score ( IPSS) ,blood pres-sure,maximal urinary flow rate (Qmax)and the adverse reactions before the treatment,at the end of the 1st,3rd month of treatment were observed and recorded. Results At the end of the 3rd month of treatment,the systolic and diastolic blood pressures of the patients in the two groups were significantly lower (P0. 05 ) . Conclusion Terazosin in the treatment of BPH complicated with hypertension can improve the lower urinary tract symptoms and the life quality of the patients. It can help control the blood pressure of the patients with good safety and compliance,it is worthy of clinical application.%目的:评价特拉唑嗪治疗良性前列腺增生( BPH)合并原发性高血压患者的有效性、安全性及依从性。方法选择200例BPH合并原发性高血压患者作为研究对象,将其随机分为观察组和对照组,每组100例。对照组患者给予常规降压药物治疗,观察组患者在对照组的基础上加用口服特拉唑嗪,随访3个月。治疗前、治疗第1个月末、治疗第3个月末,记录两组患者的国际前列腺症状评分( IPSS)变化值、血压、最大尿流率( Qmax)的变化及不良反应发生情况。结果治疗第3个月末,两组患者的收缩压和舒张压均较治疗前显著下降(P0.05)。结论特拉唑嗪治疗BPH合并高血压可

  2. 经直肠实时超声弹性成像对前列腺增生和前列腺癌鉴别诊断的价值%The value of transrectal real-time ultrasonic elastography in the differential diagnosis between BPH and PCa

    Institute of Scientific and Technical Information of China (English)

    肖世伟; 左毅刚; 王剑松; 丁明霞; 冯蕾; 刘松

    2013-01-01

    Objective:To investigate the value of transrectal real-time ultrasonic elastography (TRTE) in the differential diagnosis between BPH and PCa,and the correlation of TRTE with Gleason scores and clinical stages.Method:Patients that were doubtful of PCa underwent a conventional TRTE check between October 2011 and September 2012 in our hospital.The results of strain ratio (SR) gained from quantitative analysis and elastic scores gained according to Ophir prostate five points-elastic scores were compared and analyzed with pathologic results and clinical stages to investigate the value of TRTE for BPH and PCa differential diagnosis.Result:The area under the Receiver Operating Characteristic curves (ROC curves) of SR max were 0.704 for differential diagnosis of BPH and PCa(95%CI 0.570-0.837,P<0.01),when a cutoff point of 16.50 was used,SR max had a sensitivity of 64.50% and a specificity 82.9% ; the area under the ROC curves of SR min were 0.723 for differential diag nosis of BPH and PCa(95%CI 0.592-0.853,P<0.01),when a cutoff point of 10.76 was used,SR min had a sensitivity of 61.30% and a specificity 85.7% ; when a cutoff point of 3 was used,elastic score had a sensitivity of 83.90% and a specificity of 65.70%.SR max has a low positive correlation with.Gleason scores (r=0.322,P=0.038); SR min has a moderately positive correlation with Gleason scores (r=0.413,P=0.021); SR max and SR min has no significant statistically significant correlation with clinical stages(r=-0.114,P =0.543; r=-0.060,P=0.748).Elastic score has a moderately positive correlation with Gleason scores and clinical stages respectively(r=0.450,P=0.011; r=0.595,P<0.001).Conclusion:TRTE is valuahle for differential diagnosis of BPH and PCa,and it has a certain degree of correlation with Gleason scores and clinical stages.%目的:探讨经直肠实时超声弹性成像(transrectal real-time ultrasonic elastography,TRTE)对前列腺增生(BPH)和前列腺癌(PCa)鉴别诊断的价值

  3. 丹七川故饮合深部热疗治疗良性前列腺增生伴膀胱过度活动症的临床观察%Clinical observation on treating BPH concomitant with OAB in Dan-qi-chuan-gu-yin plus deep-part thermotherapy

    Institute of Scientific and Technical Information of China (English)

    田亚伟; 夏阳; 王文杰

    2015-01-01

    目的:探讨丹七川故饮合深部热疗治疗良性前列腺增生伴膀胱过度活动症的临床疗效和安全性。方法:选择BPH伴OAB患者23例,口服自拟中药方丹七川故饮和进行深部热疗12周,治疗前后分别评价IPSS、OABSS、Qmax、尿急次数、日间排尿、夜尿等指标。结果:治疗后IPSS评分、OABSS评分、Qmax、尿急次数、日间排尿次数、夜尿次数等指标均较治疗前有改善,有统计学意义(P<0.01)。23例患者均未出现尿储留及不良反应。结论:丹七川故饮合深部热疗治疗良性前列腺增生伴膀胱过度活动症患者疗效满意,安全性高。%Objective: To observe the clinical effect and safety of treating BPH concomitant with OAB in Dan-qi-chuan-gu-yin plus deep-part thermotherapy. Methods:23cases were treated with Dan-qi-chuan-gu-yin plus deep-part thermotherapy 12 weeks. IPSS, OABSS, Qmax, urgency, urination in the day and night urination respectively were evaluated before and after treatment. Results: The above indexes are better in pretreatment than that of posttreatment (P<0.01).Urinary retention and adverse reactions do not appear in all 23 cases. Conclusion: Dan-qi-chuan-gu-yin plus deep-part thermotherapy has positive curative effect and safety on treating BPH concomitant with OAB.

  4. Systematic study of spin crossover and structure in [Co(terpyRX)2](Y)2 systems (terpyRX = 4'-alkoxy-2,2':6',2''-terpyridine, X = 4, 8, 12, Y = BF4(-), ClO4(-), PF6(-), BPh4(-))

    DEFF Research Database (Denmark)

    Nielsen, Pia; Nielsen, Hans Toftlund; Bond, Andrew;

    2009-01-01

    simulation of the EPR spectra of frozen solutions of [Co(terpyR8)(2)](BF(4))(2) x H(2)O, recorded at S-, X-, and Q-band frequencies, allowed accurate determination of the g and A values of the low spin ground state. The temperature dependence of the polycrystalline powder EPR spectra of this and the R4 and R......A family of spin crossover cobalt(II) complexes of the type [Co(terpyRX)(2)](Y)(2) x nH(2)O (X = 4, 8, 12 and Y = BF(4)(-), ClO(4)(-), PF(6)(-), BPh(4)(-)) has been synthesized, whereby the alkyl chain length, RX, and counteranion, Y, have been systematically varied. The structural (single crystal......12 complexes is explained in terms of Jahn-Teller effects using the warped Mexican hat potential energy surface model perturbed by the low symmetry of the ligands. While well recognized in Cu(II) systems, this is one of the few times this approach has been used for Co(II)....

  5. BPH gene expression profile associated to prostate gland volume.

    Science.gov (United States)

    Descazeaud, Aurelien; Rubin, Mark A; Hofer, Matthias; Setlur, Sunita; Nikolaief, Nathalie; Vacherot, Francis; Soyeux, Pascale; Kheuang, Laurence; Abbou, Claude C; Allory, Yves; de la Taille, Alexandre

    2008-12-01

    The aim of the current study was to analyze gene expression profiles in benign prostatic hyperplasia and to compare them with phenotypic properties. Thirty-seven specimens of benign prostatic hyperplasia were obtained from symptomatic patients undergoing surgery. RNA was extracted and hybridized to Affymetrix Chips containing 54,000 gene expression probes. Gene expression profiles were analyzed using cluster, TreeView, and significance analysis of microarrays softwares. In an initial unsupervised analysis, our 37 samples clustered hierarchically in 2 groups of 18 and 19 samples, respectively. Five clinical parameters were statistically different between the 2 groups: in group 1 compared with group 2, patients had larger prostate glands, had higher prostate specific antigen levels, were more likely to be treated by alpha blockers, to be operated by prostatectomy, and to have major irritative symptoms. The sole independent parameter associated with this dichotome clustering, however, was the prostate gland volume. Therefore, the role of prostate volume was explored in a supervised analysis. Gene expression of prostate glands 60 mL were compared using significance analysis of microarrays and 227 genes were found differentially expressed between the 2 groups (>2 change and false discovery rate of <5%). Several specific pathways including growth factors genes, cell cycle genes, apoptose genes, inflammation genes, and androgen regulated genes, displayed major differences between small and large prostate glands.

  6. Effect of tamsulosin on ejaculatory function in BPH/LUTS

    Institute of Scientific and Technical Information of China (English)

    Sang Hoon Song; Jae Seog Hyun; Nam Cheol Park; Hwancheol Son; Kwang Taek Kim; Sae Woong Kim; Du Geon Moon; Ki Hak Moon; Kwangsung Park; Jong Kwan Park; Sung-Won Lee

    2011-01-01

    This study was undertaken to determine the impact on ejaculatory function of tamsulosin (0.2 mg) given once daily (OD) for 12 weeks and to identify risk factors for ejaculatory dysfunction in patients undergoing this treatment.Males with an International Prostatic Symptom Score (IPSS) ≥8 were enrolled in this study.All participants completed questionnaires,including the IPSS and the Male Sexual Health Questionnaire (MSHQ),and serum prostate-specific antigen,transrectal ultrasound and uroflowmetry with post-void residual were measured.After initiating 0.2 mg OD tamsulosin,patients were re-evaluated on the fourth and twelfth weeks of medication.The chi-squared test,the independent t-test and one-way ANOVA were used to compare means.Binary logistic regression analysis was used to calculate the odds ratio for all risk factors.A total of 177 men constituted the study cohort.No significant difference was observed between baseline and follow-up for the erectile function,ejaculatory function,satisfaction,sexual activity and desire domains (EFD,EjFD,SDA and ADD) or for erectile or ejaculatory bother mean scores.After 12 weeks,the overall incidence of ejaculatory dysfunction (EjD) was 13.4%.Incidences of the seven different types of EjD (decreased frequency,delay,dryness,decreased strength/force,decreased volume,decreased pleasure and pain at ejaculation) were 2.4%,3.1%,3.9%,3.9%,6.3%,7.1% and 3.1%,respectively.Baseline EjFD scores were higher for I PSS responders than for non-responders (26.09 vs.24.06,P=0.03).An EjFD score reduction was more frequent in IPSS responders.The incidence of EjD was small,but not negligible and was more frequent in patients with less lower urinary tract symptoms,a smaller prostate,higher baseline MSHQ totals and higher EjFD scores.

  7. Treating Enlarged Prostate (BPH): Which Drugs Work Best

    Science.gov (United States)

    ... prescribed. C Prices are based on nationwide average retail prices for March 2012. Consumer Reports Best Buy Drugs obtained prices from data provided by Source Healthcare Analytics, LLC., which is not involved in our analysis ...

  8. Numerical simulations of a diode laser BPH treatment system

    Energy Technology Data Exchange (ETDEWEB)

    Esch, V; London, R A; Papademetriou, S

    1999-02-23

    Numerical simulations are presented of the laser-tissue interaction of a diode laser system for treating benign prostate hyperplasia. The numerical model includes laser light transport, heat transport, cooling due to blood perfusion, thermal tissue damage, and enthalpy of tissue damage. Comparisons of the simulation results to clinical data are given. We report that a reasonable variation from a standard set of input data produces heating times which match those measured in the clinical trials. A general trend of decreasing damage volume with increasing heating time is described. We suggest that the patient-to- patient variability seen in the data can be explained by differences in fundamental biophysical properties such as the optical coefficients. Further work is identified, including the measurement and input to the model of several specific data parameters such as optical coefficients, blood perfusion cooling rate, and coagulation rates.

  9. "回缩液"治疗前列腺增生症(BPH)%"Restoring Injection" Treating Benign Prostatic Hypertrophy (BPH)

    Institute of Scientific and Technical Information of China (English)

    黄志华; 张梅贞

    1999-01-01

    采用以中药为主配成"回缩液"直接注射到前列腺体内,使增生的前列腺逐渐"回缩",从而解除因前列腺增生压迫后尿道引起的排尿障碍,恢复正常的排尿功能.

  10. Systematic study of spin crossover and structure in [Co(terpyRX)2](Y)2 systems (terpyRX = 4'-alkoxy-2,2':6',2''-terpyridine, X = 4, 8, 12, Y = BF4(-), ClO4(-), PF6(-), BPh4(-)).

    Science.gov (United States)

    Nielsen, Pia; Toftlund, Hans; Bond, Andrew D; Boas, John F; Pilbrow, John R; Hanson, Graeme R; Noble, Christopher; Riley, Mark J; Neville, Suzanne M; Moubaraki, Boujemaa; Murray, Keith S

    2009-08-03

    A family of spin crossover cobalt(II) complexes of the type [Co(terpyRX)(2)](Y)(2) x nH(2)O (X = 4, 8, 12 and Y = BF(4)(-), ClO(4)(-), PF(6)(-), BPh(4)(-)) has been synthesized, whereby the alkyl chain length, RX, and counteranion, Y, have been systematically varied. The structural (single crystal X-ray diffraction) and electronic (magnetic susceptibility, electron paramagnetic resonance (EPR)) properties have been investigated within this family of compounds. Single crystal X-ray diffraction analysis of [Co(terpyR8)(2)](ClO(4))(2), [Co(terpyR8)(2)](BF(4))(2) x H(2)O, and [Co(terpyR4)(2)](PF(6))(2) x 3 H(2)O, at 123 K, revealed compressed octahedral low spin Co(II) environments and showed varying extents of disorder in the alkyl tail portions of the terpyRX ligands. The magnetic and EPR studies were focused on the BF(4)(-) family and, for polycrystalline solid samples, revealed that the spin transition onset temperature (from low to high spin) decreased as the alkyl chain lengthened. EPR studies of polycrystalline powder samples confirmed these results, showing signals only due to the low spin state at the temperatures seen in magnetic measurements. Further to this, simultaneous simulation of the EPR spectra of frozen solutions of [Co(terpyR8)(2)](BF(4))(2) x H(2)O, recorded at S-, X-, and Q-band frequencies, allowed accurate determination of the g and A values of the low spin ground state. The temperature dependence of the polycrystalline powder EPR spectra of this and the R4 and R12 complexes is explained in terms of Jahn-Teller effects using the warped Mexican hat potential energy surface model perturbed by the low symmetry of the ligands. While well recognized in Cu(II) systems, this is one of the few times this approach has been used for Co(II).

  11. Alpha blocker prazosin for the treatment of benign prostatic hypertrophy (BPH.

    Directory of Open Access Journals (Sweden)

    Punekar S

    1995-10-01

    Full Text Available In this study medical treatment with alpha blocker-prazosin is compared with transurethral resection of prostate (TURP in 62 patients suffering from benign enlargement of prostate with a gland size of less than 20 gms. After thorough interrogation patients were offered either TURP or prazosin therapy. Symptom scoring, residual volume of urine and urinary flow rates were estimated in both the groups before and 3 months after the therapy. 23.5% patients in prazosin group while 90% of patients in TURP group had significant improvement. This distinctly brings out the superiority of TURP for benign enlargement of prostate.

  12. Finasterid ved symptomgivende benign prostatahypertrofi. Et toårs placebokontrolleret studie. Skandinaviske BPH-Studiegruppe

    DEFF Research Database (Denmark)

    Andersen, J T; Wolf, H; Ekman, P;

    1996-01-01

    The efficacy and safety of treatment with finasteride 5 mg daily for 24 months was assessed in this multicentre double blind placebo-controlled study including 707 patients with moderately symptomatic benign prostatic hyperplasia. Efficacy parameters were changes in voiding- and bladder storage...... symptoms assessed by a validated symptom score, changes in maximum urinary flow rate and changes in the prostate volume. In the finasteride patients, symptom score improved during the whole study with a significant difference between active treatment and placebo after 24 months (p ... rate increased in finasteride treated patients resulting in a difference between these and the placebo treated patients of 1.8 ml/s after 24 months (p Prostate volume was reduced by 19% in the finasteride treated patients versus an increase of 12% in the placebo treated patient group (p

  13. Prostatic Artery Embolization (PAE) for Symptomatic Benign Prostatic Hyperplasia (BPH): Part 2, Insights into the Technical Rationale.

    Science.gov (United States)

    Sun, Fei; Crisóstomo, Verónica; Báez-Díaz, Claudia; Sánchez, Francisco M

    2016-02-01

    Rationale of prostatic artery embolization (PAE) in the treatment of symptomatic benign prostatic hyperplasia is conventionally believed to include two parts: shrinkage of the enlarged prostate gland as a result of PAE-induced ischemic infarction and potential effects to relax the increased prostatic smooth muscle tone by reducing the number and density of α1-adrenergic receptor in the prostate stroma. This review describes new insights into the likely mechanisms behind PAE, such as ischemia-induced apoptosis, apoptosis enhanced by blockage of androgens circulation to the embolized prostate, secondary denervation following PAE, and potential effect of nitric oxide pathway immediately after embolization. Studies on therapeutic mechanisms in PAE may shed light on potentially new treatment strategies and development of novel techniques.

  14. Prostatic Artery Embolization (PAE) for Symptomatic Benign Prostatic Hyperplasia (BPH): Part 2, Insights into the Technical Rationale

    Energy Technology Data Exchange (ETDEWEB)

    Sun, Fei, E-mail: feisun@ccmijesususon.com; Crisóstomo, Verónica, E-mail: crisosto@ccmijesususon.com; Báez-Díaz, Claudia, E-mail: cbaez@ccmijesususon.com; Sánchez, Francisco M., E-mail: msanchez@ccmijesususon.com [Jesús Usón Minimally Invasive Surgery Centre (Spain)

    2016-02-15

    Rationale of prostatic artery embolization (PAE) in the treatment of symptomatic benign prostatic hyperplasia is conventionally believed to include two parts: shrinkage of the enlarged prostate gland as a result of PAE-induced ischemic infarction and potential effects to relax the increased prostatic smooth muscle tone by reducing the number and density of α{sub 1}-adrenergic receptor in the prostate stroma. This review describes new insights into the likely mechanisms behind PAE, such as ischemia-induced apoptosis, apoptosis enhanced by blockage of androgens circulation to the embolized prostate, secondary denervation following PAE, and potential effect of nitric oxide pathway immediately after embolization. Studies on therapeutic mechanisms in PAE may shed light on potentially new treatment strategies and development of novel techniques.

  15. Invasively estimated international continence society obstruction classification versus noninvasively assessed bladder outlet obstruction probability in treatment recommendation for LOTS suggestive of BPH

    NARCIS (Netherlands)

    Boormans, Joost L.; van Venrooij, Ger E. P. M.; Boon, Tom A.

    2007-01-01

    OBJECTIVES To investigate the contribution of urodynamically proven presence or absence (International Continence Society classification) of bladder outlet obstruction (BOO) to treatment recommendations for lower urinary tract symptoms (LUTS) suggestive of benign prostatic hyperplasia, and to invest

  16. GenBank blastx search result: AK062096 [KOME

    Lifescience Database Archive (English)

    Full Text Available AK062096 001-044-H12 M86348.1 Pseudomonas sp. LB400 biphenyl dioxygenase (bphA), biphenyl dioxygenase (bph...E), biphenyl dioxygenase (bphF) and biphenyl dioxygenase (bphG)s, complete cds, and dihydrodiol dehydrogenase (bphB), partial cds.|BCT BCT 1e-14 +3 ...

  17. Wirksamkeit und Verträglichkeit von Mepartricin 3 x 50.000 IE täglich versus Mepartricin 1 x 150.000 IE täglich in der Therapie von Patienten mit unterer Harntraktsymptomatik bei BPH

    Directory of Open Access Journals (Sweden)

    Priemer V

    2001-01-01

    Full Text Available Ziel der Studie ist es, die Wirksamkeit und Verträglichkeit von Mepartricin in der neuen Einmaldosierung (150.000 IE = 40 mg mit der bisherigen 3x täglichen Dosierung (zu jeweils 50.000 IE zu vergleichen. Material und Methoden: 170 Patienten wurde in die Studie aufgenommen. Die Patientendaten wurden aus 5 verschiedenen Doppelblindstudien, die den gleichen Fragebogen benutzten, gepoolt und in der aktuellen Studie analysiert. 70 Patienten waren in der Gruppe, die 3x täglich 50.00 IE Mepartricin erhielten und 100 Patienten waren in der Gruppe, die 1x täglich 150.000 IE Mepartricin erhielten. Eingangs, ein sowie zwei Monate nach Studieneintritt wurden die Patienten bezüglich Nykturie, Pollakisurie und Dysurie evaluiert. Weiters wurden jeweils der Uroflow, Restharn, Nebenwirkungen sowie das Serumcholesterin analysiert. Ergebnisse: Das mittlere Alter der Patienten in der 3 x 50.000 IE Gruppe war 62,8 a, in der 1 x 150.000 IE Gruppe 65,7a (p 0,05. Die Nykturie nahm innerhalb zweier Monate in der 3 x 50.000 IE Gruppe von 3,8 auf 1,4 ab, in der 1 x 150.000 E Gruppe nahm sie sogar von 3,9 auf 0,9 ab. Damit ist der Rückgang in der mit 1 x 150.000 IE behandelten Gruppe signifikant besser als in der 3 x 50.000 IE Vergleichsgruppe (p = 0,001. Die Pollakisurie reduzierte sich in der 3 x 50000 IE Gruppe von 8,3 auf 5,2, in der 1 x 150.000 IE Gruppe von 8,5 auf 4,9 (p = 0,106. Im Bereich der Dysurie zeigte sich nach 2 Monaten ein hochsignifikanter Unterschied zugunsten der 1 x 150.000 IE Form (p = 0,002. Während in der 3 x 50.000 IE Gruppe der Qmax von 8,6 auf 12 ml/sek stieg, stieg er in der 1 x 150.000 IE Gruppe von 7,4 auf 11,9 ml/sek. Im gleichen Zeitraum (2 Monate reduzierte sich der Restharn in der 3 x 50.000 IE Gruppe von 78 auf 46 ml, in der 1 x 150.000 IE Gruppe sank er von 82 auf 36 ml (p = 0,056. Die Nebenwirkungen betrafen den Gastrointestinaltrakt und betrugen 10 % bzw. 12 % (p = 0,9. Schlußfolgerung: Diese gepoolte Analyse zeigt, daß beide Formen therapeutisch äquivalent, die neue Einmaldosierung in einigen Parametern signifikant besser wirksam war.

  18. An experiental study of photon irradiates for the cultured cells of BPH%光子对前列腺增生培养细胞照射的实验研究

    Institute of Scientific and Technical Information of China (English)

    黄恒前; 莫曾南; 杨小丽; 庞友红

    2008-01-01

    目的:观察Dolphin-2000型前列腺光子治疗机照射对前列腺增生组织培养细胞的作用及其可能机制.方法:采用Dolphin-2000型前列腺光子治疗机对前列腺增生组织培养细胞照射后经MTT法检测其增殖情况.结果:与对照组(未照射)相比,在照射剂量484 CGY~522 CGY范围,光子照射对前列腺增生组织培养细胞(上皮细胞和成纤维细胞)增殖有明显抑制作用.结论:Dolphin-2000型前列腺光子治疗机照射能抑制前列腺增生组织细胞增殖.

  19. Transurethral Resection of the Prostate (TURP) Versus Original and PErFecTED Prostate Artery Embolization (PAE) Due to Benign Prostatic Hyperplasia (BPH): Preliminary Results of a Single Center, Prospective, Urodynamic-Controlled Analysis

    Energy Technology Data Exchange (ETDEWEB)

    Carnevale, Francisco C., E-mail: fcarnevale@uol.com.br [University of Sao Paulo Medical School, Interventional Radiology Unit (Brazil); Iscaife, Alexandre, E-mail: iscaifeboni@yahoo.com.br; Yoshinaga, Eduardo M., E-mail: dumuracca@ig.com.br [University of Sao Paulo Medical School, Division of Urology (Brazil); Moreira, Airton Mota, E-mail: motamoreira@gmail.com [University of Sao Paulo Medical School, Interventional Radiology Unit (Brazil); Antunes, Alberto A., E-mail: antunesuro@uol.com.br; Srougi, Miguel, E-mail: srougi@uol.com.br [University of Sao Paulo Medical School, Division of Urology (Brazil)

    2016-01-15

    PurposeTo compare clinical and urodynamic results of transurethral resection of the prostate (TURP) to original and PErFecTED prostate artery embolization (PAE) methods for benign prostatic hyperplasia.MethodsWe prospectively randomized 30 patients to receive TURP or original PAE (oPAE) and compared them to a cohort of patients treated by PErFecTED PAE, with a minimum of 1-year follow-up. Patients were assessed for urodynamic parameters, prostate volume, international prostate symptom score (IPSS), and quality of life (QoL).ResultsAll groups were comparable for all pre-treatment parameters except bladder contractility and peak urine flow rate (Q{sub max}), both of which were significantly better in the TURP group, and IIEF score, which was significantly higher among PErFecTED PAE patients than TURP patients. All groups experienced significant improvement in IPSS, QoL, prostate volume, and Q{sub max}. TURP and PErFecTED PAE both resulted in significantly lower IPSS than oPAE but were not significantly different from one another. TURP resulted in significantly higher Q{sub max} and significantly smaller prostate volume than either original or PErFecTED PAE but required spinal anesthesia and hospitalization. Two patients in the oPAE group with hypocontractile bladders experienced recurrence of symptoms and were treated with TURP. In the TURP group, urinary incontinence occurred in 4/15 patients (26.7 %), rupture of the prostatic capsule in 1/15 (6.7 %), retrograde ejaculation in all patients (100 %), and one patient was readmitted for temporary bladder irrigation due to hematuria.ConclusionsTURP and PAE are both safe and effective treatments. TURP and PErFecTED PAE yield similar symptom improvement, but TURP is associated with both better urodynamic results and more adverse events.

  20. The application of UPP in stent of prostate of BPH%尿道压力分布定位在前列腺支架置入术中的应用

    Institute of Scientific and Technical Information of China (English)

    何小舟; 巢志复; 许滔

    2000-01-01

    目的为了更好地解决前列腺增生患者支架置入术中,前列腺增生梗阻的长度、部位与支架相匹配的问题.方法经尿道测压(UPP)检查测定前列腺尿道长度,据此选择合适的镍钛记忆合金支架,行前列腺支架置入术.结果手术均一次成功,术后病人恢复自行排尿,最大尿流率(MFR)由术前4.2±2.0 ml/s上升为12.5±2.5 ml/s,平均尿流率(AFR)由术前1.8±0.8 ml/s上升为4.0±1.5 ml/s,残余尿由术前平均下降至40~90 ml,有显著差异(P<0.01).结论 UPP检查能准确地判定前列腺尿道的长度及压力,为支架准备提供精确的依据,并能很好地判断术后支架的位置和疗效.

  1. Characterization of the molecular degradation mechanism of diphenyl ethers by Cupriavidus sp. WS.

    Science.gov (United States)

    Wang, Sheng; Bai, Naling; Wang, Bing; Feng, Zhuo; Hutchins, William C; Yang, Ching-Hong; Zhao, Yuhua

    2015-11-01

    Commonly used flame retardants, such as polybrominated diphenyl ethers, are extremely persistent in the environment, causing serious environmental risks. Certain strains of bacteria are able to degrade several low brominated congeners of PBDEs aerobically. However, the aerobic degradation pathway is not yet well understood, particularly at the genetic level. In this study, we isolated Cupriavidus sp. WS from the environment that could degrade diphenyl ether (DE), 4-bromodiphenyl ether, and 4,4'-bromodiphenyl ether. DE was completely degraded in 6 days without any detectable end-product. Using transposon mutagenesis, several DE degradation-deficient mutants were obtained. Knocking out bphA1, bphA2, and bphA3 eliminated the ability of the Cupriavidus sp. WS bacterium to degrade DE, indicating that the bph genes play a crucial role in DE degradation by this strain. The specific roles of bphA, bphB, and bphC were identified by systematically expressing these genes in Escherichia coli. The dihydrodiol product of BphA was dehydrogenated into 2,3-dihydroxydiphenyl ether by BphB. 2,3-Dihydroxydiphenyl ether was then decomposed into phenol and 2-pyrone-6-carboxylic acid by BphC. Thus, BphA, BphB, and BphC act sequentially in the aerobic degradation of DE, 4-bromodiphenyl ether, and 4,4'-dibromodiphenyl ether by the Cupriavidus sp. WS bacterium.

  2. Role of Acoustic Radiation Force Impulse (ARFI) Elastography in Determination of Severity of Benign Prostate Hyperplasia

    Science.gov (United States)

    Alan, Bircan; Utangaç, Mazhar; Göya, Cemil; Dağgülli, Mansur

    2016-01-01

    Background The aim of this study was to investigate the potential contribution of acoustic radiation force impulse (ARFI) elastography to the determination of the severity of benign prostate hypertrophy (BPH) by performing shear wave velocity (SWV) measurements of the prostate using ARFI technology. Material/Methods Sixty BPH patients and 40 healthy volunteers were included in this study. SWV measurements of the prostate were performed by transabdominal ultrasonography (US), both in the BPH patients and control subjects. The BPH patients also underwent uroflowmetry measurements. Using the International Prostate Symptom Score (IPSS), the BPH patients were divided into two subgroups, a mild-to-moderate BPH group and a severe BPH group, to compare SWV values. Results The BPH patients had higher SWV values for the central area of the prostate compared to the control subjects (2.52±0.59 m/s and 1.47±0.42 m/s, pprostate were higher in the severe BPH group compared to the mild-to-moderate BPH group (2.62±0.58 and 2.25±0.55, p=0.02). Conclusions Our ARFI elastography results indicated that the central prostate SWV values of BPH patients were significantly higher relative to those of a healthy control group. The central prostate SWV values increased in proportion to the increased severity of BPH. Measurement of SWV by ARFI technology constitutes a non-invasive alternative to other methods for the determination of BPH severity. PMID:27876713

  3. Genetic basis of multiple resistance to the brown planthopper (Nilaparvata lugens Stål) and the green rice leafhopper (Nephotettix cincticeps Uhler) in the rice cultivar 'ASD7' (Oryza sativa L. ssp. indica).

    Science.gov (United States)

    Van Mai, Tan; Fujita, Daisuke; Matsumura, Masaya; Yoshimura, Atsushi; Yasui, Hideshi

    2015-12-01

    The rice cultivar ASD7 (Oryza sativa L. ssp. indica) is resistant to the brown planthopper (BPH; Nilaparvata lugens Stål) and the green leafhopper (Nephotettix virescens Distant). Here, we analyzed multiple genetic resistance to BPH and the green rice leafhopper (GRH; Nephotettix cincticeps Uhler). Using two independent F2 populations derived from a cross between ASD7 and Taichung 65 (Oryza sativa ssp. japonica), we detected two QTLs (qBPH6 and qBPH12) for resistance to BPH and one QTL (qGRH5) for resistance to GRH. Linkage analysis in BC2F3 populations revealed that qBPH12 controlled resistance to BPH and co-segregated with SSR markers RM28466 and RM7376 in plants homozygous for the ASD7 allele at qBPH6. Plants homozygous for the ASD7 alleles at both QTLs showed a much faster antibiosis response to BPH than plants homozygous at only one of these QTLs. It revealed that epistatic interaction between qBPH6 and qBPH12 is the basis of resistance to BPH in ASD7. In addition, qGRH5 controlled resistance to GRH and co-segregated with SSR markers RM6082 and RM3381. qGRH5 is identical to GRH1. Thus, we clarified the genetic basis of multiple resistance of ASD7 to BPH and GRH.

  4. Genetic basis of multiple resistance to the brown planthopper (Nilaparvata lugens Stål) and the green rice leafhopper (Nephotettix cincticeps Uhler) in the rice cultivar ‘ASD7’ (Oryza sativa L. ssp. indica)

    Science.gov (United States)

    Van Mai, Tan; Fujita, Daisuke; Matsumura, Masaya; Yoshimura, Atsushi; Yasui, Hideshi

    2015-01-01

    The rice cultivar ASD7 (Oryza sativa L. ssp. indica) is resistant to the brown planthopper (BPH; Nilaparvata lugens Stål) and the green leafhopper (Nephotettix virescens Distant). Here, we analyzed multiple genetic resistance to BPH and the green rice leafhopper (GRH; Nephotettix cincticeps Uhler). Using two independent F2 populations derived from a cross between ASD7 and Taichung 65 (Oryza sativa ssp. japonica), we detected two QTLs (qBPH6 and qBPH12) for resistance to BPH and one QTL (qGRH5) for resistance to GRH. Linkage analysis in BC2F3 populations revealed that qBPH12 controlled resistance to BPH and co-segregated with SSR markers RM28466 and RM7376 in plants homozygous for the ASD7 allele at qBPH6. Plants homozygous for the ASD7 alleles at both QTLs showed a much faster antibiosis response to BPH than plants homozygous at only one of these QTLs. It revealed that epistatic interaction between qBPH6 and qBPH12 is the basis of resistance to BPH in ASD7. In addition, qGRH5 controlled resistance to GRH and co-segregated with SSR markers RM6082 and RM3381. qGRH5 is identical to GRH1. Thus, we clarified the genetic basis of multiple resistance of ASD7 to BPH and GRH. PMID:26719745

  5. Role of Acoustic Radiation Force Impulse (ARFI) Elastography in Determination of Severity of Benign Prostate Hyperplasia.

    Science.gov (United States)

    Alan, Bircan; Utangaç, Mazhar; Göya, Cemil; Dağgülli, Mansur

    2016-11-23

    BACKGROUND The aim of this study was to investigate the potential contribution of acoustic radiation force impulse (ARFI) elastography to the determination of the severity of benign prostate hypertrophy (BPH) by performing shear wave velocity (SWV) measurements of the prostate using ARFI technology. MATERIAL AND METHODS Sixty BPH patients and 40 healthy volunteers were included in this study. SWV measurements of the prostate were performed by transabdominal ultrasonography (US), both in the BPH patients and control subjects. The BPH patients also underwent uroflowmetry measurements. Using the International Prostate Symptom Score (IPSS), the BPH patients were divided into two subgroups, a mild-to-moderate BPH group and a severe BPH group, to compare SWV values. RESULTS The BPH patients had higher SWV values for the central area of the prostate compared to the control subjects (2.52±0.59 m/s and 1.47±0.42 m/s, pprostate were higher in the severe BPH group compared to the mild-to-moderate BPH group (2.62±0.58 and 2.25±0.55, p=0.02). CONCLUSIONS Our ARFI elastography results indicated that the central prostate SWV values of BPH patients were significantly higher relative to those of a healthy control group. The central prostate SWV values increased in proportion to the increased severity of BPH. Measurement of SWV by ARFI technology constitutes a non-invasive alternative to other methods for the determination of BPH severity.

  6. Bacterial Community in Different Populations of Rice Brown Planthopper Nilaparvata lugens (Stål)

    Institute of Scientific and Technical Information of China (English)

    XU Hong-xing; ZHENG Xu-song; YANG Ya-jun; WANG Xin; YE Gong-yin; LU Zhong-xian

    2014-01-01

    The structures of bacterial communities in the brown planthopper (BPH) Nilaparvata lugens (Stål) from different geographic and resistant virulent populations were analyzed by using denatured gradient gel electrophoresis (DGGE). Results showed that the bacterial communities in BPH nymph from the first to the fifth instars varied with nymphal growth and development. The bacterial communities in the first-instar BPH nymph were similar to those in adults. Nine geographic BPH populations were divided into three groups based on the cluster analysis of DGGE fingerprint. The first group was from the Philippines;the second group was from Thailand and Hainan, Yunnan and Zhejiang provinces of China; and the third group was from Vietnam and Guangxi, Hunan and Jiangxi provinces of China. BPH populations adapted to different resistant rice varieties. The BPH populations from Mudgo (with resistant gene Bph1) and ASD7 (with resistant gene bph2) differed with those of the susceptible rice variety TN1.

  7. Risk stratification for benign prostatic hyperplasia.

    Science.gov (United States)

    Zattoni, Fabio; Ficarra, Vincenzo; Novara, Giacomo

    2017-03-18

    Benign prostatic hyperplasia (BPH) represents an important public health problem in ageing men due to frequently associated lower urinary tract symptoms (LUTS), which may impair quality of life. BPH is also a progressive disease, mainly characterized by a worsening of LUTS over time, and in some patients by the occurrence of serious outcomes such as acute urinary retention and need for BPH-related surgery. The management of BPH and LUTS in men should move forward its focus on symptom control only. Indeed, the goals of therapy for BPH are not only to improve bothersome LUTS but also to identify those patients at risk of unfavourable outcomes in order to optimize their management and reduce complications. Risk stratification and tailored treatment should improve the reductions in both symptoms and the long-term consequences of BPH and BPH treatments. To do this, clinicians need to know possible factors that may support the develop of PBH and possible risks due to the BPH itself.

  8. Androgen Receptor Roles in the Development of Benign Prostate Hyperplasia

    OpenAIRE

    IZUMI, KOUJI; Mizokami, Atsushi; Lin, Wen-Jye; Lai, Kuo-Pao; Chang, Chawnshang

    2013-01-01

    Benign prostate hyperplasia (BPH) is a major cause of lower urinary tract symptoms, with an increased volume of transitional zone and associated with increased stromal cells. It is known that androgen/androgen receptor (AR) signaling plays a key role in development of BPH, and that blockade of this signaling decreases BPH volume and can relieve lower urinary tract symptoms, but the mechanisms of androgen/AR signaling in BPH development remain unclear, and the effectiveness of current drugs fo...

  9. Cost effectiveness comparison of dutasteride and finasteride in patients with benign prostatic hyperplasia - the Markov model based on data from Montenegro

    OpenAIRE

    Dabanović Vera; Kostić Marina; Janković Slobodan

    2016-01-01

    Background/Aim. Benign prostatic hyperplasia (BPH) is one of the most common disease among males aging 50 years and more. The rise of the prevalence of BPH is related to aging, and since duration of life time period has the tendency of rising the prevalence of BPH will rise as costs of BPH treatment will and its influence on health economic budget. Dutasteride is a new drug similar to finasteride, inhibits enzyme testosterone 5-alpha reductase, diminish sym...

  10. Diet-dependent fecundity of the spiders Atypena formosana and Pardosa pseudoannulata, predators in irrigated rice

    DEFF Research Database (Denmark)

    Sigsgaard, Lene; Toft, Søren; Villareal, Sylvia

    2001-01-01

    The fecundity of the spiders Atypena formosana and Pardosa pseudoannulata was assessed on diets of brown planthopper Nilaparvata lugens (BPH), green leafhopper Nephotettix virescens (GLH), Collembola (Entomobryidae), Drosophila melanogaster and three prey mixtures; BPH-GLH, BPH-GLH-Collembola and...

  11. The correlation between metabolic syndrome and benign prostatic hyperplasia and drug efficacy evaluation

    Institute of Scientific and Technical Information of China (English)

    李欣

    2014-01-01

    Objective To explore the correlation between metabolic syndrome(MS)and benign prostatic hyperplasia(BPH)and the drug treatment of BPH.Methods 300cases of BPH patients treated between May 2011 to June2012 in our hospital and Tonghai County hospitals of traditonal Chinese medicine were included.The patients were divided into observing group(n=100)and control

  12. Correlation between benign prostate hyperplasia and the related indicators of atherosclerosis

    Institute of Scientific and Technical Information of China (English)

    张瑞华

    2012-01-01

    Objective To investigate the correlation between benign prostate hyperplasia (BPH) and atherosclerotic related indicators. Methods Totally 125 patients were divided into two groups based on prostate volume(PV):50 cases without BPH(PV≤30 ml) and 75 cases with BPH (PV>30ml)

  13. Association of Clinical Benign Prostate Hyperplasia with Prostate Cancer Incidence and Mortality Revisited: A Nationwide Cohort Study of 3 009 258 Men

    DEFF Research Database (Denmark)

    Ørsted, David Dynnes; Bojesen, Stig E; Nielsen, Sune F;

    2011-01-01

    BACKGROUND: Although benign prostate hyperplasia (BPH) and prostate cancer (PCa) share features such as hormone-dependent growth and response to treatment with antiandrogen therapy, BPH is generally not considered a premalignant lesion. OBJECTIVE: To determine whether clinical BPH is associated...

  14. Androgen receptor roles in the development of benign prostate hyperplasia.

    Science.gov (United States)

    Izumi, Kouji; Mizokami, Atsushi; Lin, Wen-Jye; Lai, Kuo-Pao; Chang, Chawnshang

    2013-06-01

    Benign prostate hyperplasia (BPH) is a major cause of lower urinary tract symptoms, with an increased volume of transitional zone and associated with increased stromal cells. It is known that androgen/androgen receptor (AR) signaling plays a key role in development of BPH, and that blockade of this signaling decreases BPH volume and can relieve lower urinary tract symptoms, but the mechanisms of androgen/AR signaling in BPH development remain unclear, and the effectiveness of current drugs for treating BPH is still limited. The detailed mechanisms of androgen/AR signaling need to be clarified, and new therapies are needed for better treatment of BPH patients. This review focuses on roles of AR in epithelial and stromal cells in BPH development. In epithelial cells, AR may contribute to BPH development via epithelial cell-stromal cell interaction with alterations of epithelial-mesenchymal transition, leading to proliferation of stromal cells. Data from several mouse models with selective knockout of AR in stromal smooth-muscle cells and/or fibroblasts indicate that the AR in stromal cells can also promote BPH development. In prostatic inflammation, AR roles in infiltrating macrophages and epithelial and stromal cells have been linked to BPH development, which has led to discovery of new therapeutic targets. For example, targeting AR with the novel AR degradation enhancer, ASC-J9 offers a potential therapeutic approach against BPH development.

  15. Impact of primary hypertension on hematuria of the patients with benign prostatic hyperplasia

    Institute of Scientific and Technical Information of China (English)

    GUO Li-jun; TANG Yuan; GUO Chao-ming; ZHANG Xiang-hua

    2010-01-01

    Background Both benign prostatic hyperplasia (BPH) and primary hypertension are common in the elderly men. The purpose of this study was to investigate the possible effect of primary hypertension on the hematuria in patients with BPH.Methods All patients who underwent transurethral resection of prostate or opening operation had confirmed diagnoses of BPH histologically. comparative analysis of packet was used to analyze the incidence of hematuria in 423 BPH patients with or without hypertension. Immunostaining of CD34 and vascular endothelial growth factor (VEGF) was carried out in tissues of 50 cases of simple BPH and 50 cases of BPH accompanied with hypertension. Results The incidence of hematuria in the BPH with hypertension was significantly higher than that in the simple BPH (P<0.01). Furthermore, the incidence of hematuria in patients who had hypertension for more than 10 years was clearly higher than that in the patients who had hypertension for less than 10 years (P <0.01). Both microvessel density (MVD) based on CD34 immunostaining and VEGF expression were significantly higher in the BPH tissues of patients with hypertension than that in the simple BPH (P<0.01, P<0.05).Conclusions Long-term hypertension may significantly increase the incidence of hematuria in patients with both BPH and hypertension. Increased MVD level and VEGF expression may account for the higher incidence of hematuria in these patients.

  16. Androgen receptor CAG polymorphism and the risk of benign prostatic hyperplasia in a Brazilian population

    Directory of Open Access Journals (Sweden)

    Vanderlei Biolchi

    2012-06-01

    Full Text Available Benign prostatic hyperplasia (BPH is a very frequent age-related proliferative abnormality in men. Polymorphic CAG repeat in the androgen receptor (AR can alter transactivation of androgen-responsive genes and potentially influence BPH risk. We investigated the association between CAG repeat length and risk of BPH in a case-control study of a Brazilian population. We evaluated 214 patients; 126 with BPH and 88 healthy controls. DNA was extracted from peripheral leucocytes and the AR gene was analyzed using fragment analysis. Hazard ratio (HR and 95% confidence interval were estimated using logistic regression models. Mean CAG length was not different between patients with BPH and controls. The CAG repeat length was examined as a categorical variable (CAG 21 and CAG 22 and did not differ between the control vs. the BPH group. We found no evidence for an association between AR CAG repeat length in BPH risk in a population-based sample of Brazilians.

  17. Current clinical management strategies for benign prostatic hyperplasia

    Institute of Scientific and Technical Information of China (English)

    ZHANG Xiang-hua

    2008-01-01

    @@ This article summarizes the major new findings on clinical management for benign prostatic hyperplasia (BPH) that were presented at the annual meeting of the American Urological Association (AUA) in May 2008.The management of symptomatic BPH has been changed significantly over the last decade in response to the availability of new treatment options.Prior to the 1980s,open prostatectomy was the only widely accepted intervention for BPH.Since then,the advent of new medical therapies for BPH and the introduction of a range of minimally invasive therapies have provided for men with lower urinary tract symptoms (LUTS) secondary to BPH.1 In this year's AUA meeting,several new findings were reported in the filed of BPH which involved epidemiology,clinical progression,drug therapy and new technologies in surgical therapy of BPH.

  18. Association of Variants in Genes Related to the Immune Response and Obesity with Benign Prostatic Hyperplasia in CLUE II

    Science.gov (United States)

    Lopez, David S.; Peskoe, Sarah B.; Tsilidis, Konstantinos K.; Hoffman-Bolton, Judy; Helzlsouer, Kathy J.; Isaacs, William B.; Smith, Michael W.; Platz, Elizabeth A.

    2014-01-01

    BACKGROUND Chronic inflammation and obesity may contribute to the genesis or progression of benign prostatic hyperplasia (BPH) and BPH-associated lower urinary tract symptoms (LUTS). The influence of variants in genes related to these states on BPH has not been studied extensively. Thus, we evaluated the association of 17 single nucleotide polymorphisms (SNPs) in immune response genes (IL1B, IL6, IL8, IL10, TNF, CRP, TLR4, RNASEL) and genes involved in obesity, including insulin regulation (LEP, ADIPOQ, PPARG, TCF7L2), with BPH. METHODS BPH cases (N=568) and age-frequency matched controls (N=568) were selected from among adult male CLUE II cohort participants who responded in 2000 to a mailed questionnaire. BPH was defined as BPH surgery, use of BPH medications, or symptomatic BPH (American Urological Association Symptom Index Score ≥15). Controls were men who had not had BPH surgery, did not use BPH medications, and whose symptom score was ≤7. Age-adjusted odds ratios (OR) and 95% confidence intervals (CI) were estimated using logistic regression. RESULTS None of the candidate SNPs was statistically significantly associated with BPH. However, we could not rule out possible weak associations for CRP rs1205 (1082C>T), ADIPOQ rs1501299 (276C>A), PPARG rs1801282 (-49C>G), and TCF7L2 rs7903146 (47833T>C). After summing risk alleles, men with ≥4 had an increased BPH risk compared with those with ≤1 (OR, 1.78; 95% CI, 1.10-2.89; Ptrend=0.006). CONCLUSION SNPs in genes related to immune response and obesity, especially in combination, may be associated with BPH. PMID:25224558

  19. Impact of Nitrogen, Phosphorus and Potassium on Brown Planthopper and Tolerance of Its Host Rice Plants

    Institute of Scientific and Technical Information of China (English)

    Md Mamunur RASHID; Mahbuba JAHAN; Khandakar Shariful ISLAM

    2016-01-01

    The brown planthopper (BPH),Nilaparvata lugens (Stål), appeared as a devastating pest of rice in Asia. Experiments were conducted to study the effects of three nutrients, nitrogen (N), phosphorus (P) and potassium (K), on BPH and its host rice plants. Biochemical constituents of BPH and rice plants with varying nutrient levels at different growth stages, and changes in relative water content (RWC) of rice plants were determined in the laboratory. Feeding of BPH and the tolerance of rice plants to BPH with different nutrient levels were determined in the nethouse. Concentrations of N and P were found much higher in the BPH body than in its host rice plants, and this elemental mismatch is an inherent constraint on meeting nutritional requirements of BPH. Nitrogen was found as a more limiting element for BPH than other nutrients in rice plants. Application of N fertilizers to the rice plants increased the N concentrations both in rice plants and BPH while application of P and K fertilizers increased their concentrations in plant tissues only but not in BPH. Nitrogen application also increased the level of soluble proteins and decreased silicon content in rice plants, which resulted in increased feeding of BPH with sharp reduction of RWC in rice plants ultimately caused susceptible to the pest. P fertilization increased the concentration of P in rice plant tissues but not changed N, K, Si, free sugar and soluble protein contents, which indicated little importance of P to the feeding of BPH and tolerance of plant against BPH. K fertilization increased K content but reduced N, Si, free sugar and soluble protein contents in the plant tissues which resulted in the minimum reduction of RWC in rice plants after BPH feeding, thereby contributed to higher tolerance of rice plants to brown planthopper.

  20. 中西药2种药物治疗方案治疗前列腺增生症的最小成本分析%Cost-minimization Analysis of 2 Drug Therapy Projects Including Traditional Chinese Medcines and Chemical Medcines in BPH Patients

    Institute of Scientific and Technical Information of China (English)

    徐航; 方芸; 黄莉莉

    2006-01-01

    目的:评价中西药2种药物治疗方案治疗前列腺增生症的经济性.方法:对60例前列腺增生症患者分别应用非那雄胺+盐酸坦索罗辛(A组)、前列康+六味地黄丸(B组)进行治疗,观察其疗效及不良反应,运用药物经济学最小成本法进行分析.结果:2组患者在治疗的有效率和不良反应方面均无显著性差异(P>0.05);成本分别为1 562.10、233.25元,有显著性差异(P<0.05).结论:前列康+六味地黄丸治疗前列腺增生症较为经济.

  1. Experience of Operative Resection-Opened of Prostate on the Benign Prostatic Hypertrophy(BPH) Patients at High Risk%开放性手术治疗56例高危前列腺增生症临床观察

    Institute of Scientific and Technical Information of China (English)

    周福荣; 庞建; 虎法静; 李苏俊; 袁波

    2003-01-01

    目的:探讨开放性前列腺切除术治疗高危前列腺增生症的安全性和疗效.方法:回顾性分析我院1990~2002年540例开放性前列腺手术资料,其中56例为高危前列腺增生症.结果:经充分术前准备、手术技巧的熟练和改进、术中充分肯定的止血,手术顺利,出血少,疗效满意,未出现严重并发症.结论:开放性前列腺切除术治疗高危前列腺患者,只要术前准备充分,手术处理仔细,术后重视,是一种安全的、并发症少、疗效确切的治疗选择.

  2. Bacteriophytochrome controls carotenoid-independent response to photodynamic stress in a non-photosynthetic rhizobacterium, Azospirillum brasilense Sp7.

    Science.gov (United States)

    Kumar, Santosh; Kateriya, Suneel; Singh, Vijay Shankar; Tanwar, Meenakshi; Agarwal, Shweta; Singh, Hina; Khurana, Jitendra Paul; Amla, Devinder Vijay; Tripathi, Anil Kumar

    2012-01-01

    Ever since the discovery of the role of bacteriophytochrome (BphP) in inducing carotenoid synthesis in Deinococcus radiodurans in response to light the role of BphPs in other non-photosynthetic bacteria is not clear yet. Azospirillum brasilense, a non-photosynthetic rhizobacterium, harbours a pair of BphPs out of which AbBphP1 is a homolog of AtBphP1 of Agrobacterium tumefaciens. By overexpression, purification, biochemical and spectral characterization we have shown that AbBphP1 is a photochromic bacteriophytochrome. Phenotypic study of the ΔAbBphP1 mutant showed that it is required for the survival of A. brasilense on minimal medium under red light. The mutant also showed reduced chemotaxis towards dicarboxylates and increased sensitivity to the photooxidative stress. Unlike D. radiodurans, AbBphP1 was not involved in controlling carotenoid synthesis. Proteome analysis of the ΔAbBphP1 indicated that AbBphP1 is involved in inducing a cellular response that enables A. brasilense in regenerating proteins that might be damaged due to photodynamic stress.

  3. Androgens and estrogens in benign prostatic hyperplasia: past, present and future.

    Science.gov (United States)

    Nicholson, Tristan M; Ricke, William A

    2011-01-01

    Benign prostatic hyperplasia (BPH) and associated lower urinary tract symptoms (LUTS) are common clinical problems in urology. While the precise molecular etiology remains unclear, sex steroids have been implicated in the development and maintenance of BPH. Sufficient data exists linking androgens and androgen receptor pathways to BPH and use of androgen reducing compounds, such as 5α-reductase inhibitors which block the conversion of testosterone into dihydrotestosterone, are a component of the standard of care for men with LUTS attributed to an enlarged prostate. However, BPH is a multifactorial disease and not all men respond well to currently available treatments, suggesting factors other than androgens are involved. Testosterone, the primary circulating androgen in men, can also be metabolized via CYP19/aromatase into the potent estrogen, estradiol-17β. The prostate is an estrogen target tissue and estrogens directly and indirectly affect growth and differentiation of prostate. The precise role of endogenous and exogenous estrogens in directly affecting prostate growth and differentiation in the context of BPH is an understudied area. Estrogens and selective estrogen receptor modulators (SERMs) have been shown to promote or inhibit prostate proliferation signifying potential roles in BPH. Recent research has demonstrated that estrogen receptor signaling pathways may be important in the development and maintenance of BPH and LUTS; however, new models are needed to genetically dissect estrogen regulated molecular mechanisms involved in BPH. More work is needed to identify estrogens and associated signaling pathways in BPH in order to target BPH with dietary and therapeutic SERMs.

  4. Measurement of benign prostatic hyperplasia treatment effects on male sexual function

    Science.gov (United States)

    Skolarus, TA; Wei, JT

    2010-01-01

    Benign prostatic hyperplasia (BPH) is the leading cause of lower urinary tract symptoms among the aging male population. Epidemiological, pathophysiological and clinical studies indicate that many of these men also suffer from declining sexual function, especially those undergoing treatment for their BPH-related urinary symptoms. Although urinary symptoms and quality of life may improve with BPH therapy, the resulting effects on sexual function vary by medical, surgical and minimally invasive approaches and have not been consistently reported. As comprehensive, validated instruments to measure male sexual function are now available for routine use in the clinical setting, urologists and primary care providers caring for patients with BPH have the opportunity to monitor both urinary and sexual function before, during and after BPH therapy. Herein, we describe the relationship between BPH and its treatments on male sexual function, the role of new measures for sexual functioning and opportunities for future work to improve the care of men suffering from both maladies. PMID:19536125

  5. Adiponectin deficiency contributes to the development and progression of benign prostatic hyperplasia in obesity

    Science.gov (United States)

    Fu, Shi; Xu, Huan; Gu, Meng; Liu, Chong; Wang, Qiong; Wan, Xiang; Chen, Yanbo; Chen, Qi; Peng, Yubing; Cai, Zhikang; Zhou, Juan; Wang, Zhong

    2017-01-01

    The incidence of benign prostatic hyperplasia (BPH) is increasing among obese individuals, but few studies have fully explained the underlying mechanisms. We aimed to elucidate the relationship between obesity and BPH. Herein, we show that in prostatic epithelial and stromal cells, adiponectin exerts multifunctional effects including anti-proliferation, blocking of G1/S-phase progression and the promotion of apoptosis via inhibiting the MEK-ERK-p90RSK axis. Furthermore, we found that a high-fat diet (HFD) led to adiponectin deficiency and microscopic BPH in a mouse model of obesity. And an adiponectin supplement protected the obese mice from microscopic BPH. The present study provides evidence that adiponectin is a protective regulator in the development and progression of BPH and that adiponectin deficiency causally links BPH with obesity. PMID:28256562

  6. Epidemiology and etiology of benign prostatic hyperplasia and bladder outlet obstruction

    Directory of Open Access Journals (Sweden)

    Nishant D Patel

    2014-01-01

    Full Text Available Benign prostatic hyperplasia (BPH is a histological diagnosis associated with unregulated proliferation of connective tissue, smooth muscle and glandular epithelium. BPH may compress the urethra and result in anatomic bladder outlet obstruction (BOO; BOO may present as lower urinary tract symptoms (LUTS, infections, retention and other adverse events. BPH and BOO have a significant impact on the health of older men and health-care costs. As the world population ages, the incidence and prevalence of BPH and LUTS have increased rapidly. Although non-modifiable risk factors - including age, genetics and geography - play significant roles in the etiology of BPH and BOO, recent data have revealed modifiable risk factors that present new opportunities for treatment and prevention, including sex steroid hormones, the metabolic syndrome and cardiovascular disease, obesity, diabetes, diet, physical activity and inflammation. We review the natural history, definitions and key risk factors of BPH and BOO in epidemiological studies.

  7. Medical and Surgical Treatment Modalities for Lower Urinary Tract Symptoms in the Male Patient Secondary to Benign Prostatic Hyperplasia: A Review.

    Science.gov (United States)

    Macey, Matthew Ryan; Raynor, Mathew C

    2016-09-01

    Benign prostatic hyperplasia (BPH) with lower urinary tract symptoms (LUTS) is one of the most common ailments affecting aging men. Symptoms typically associated with BPH include weak stream, hesitancy, urgency, frequency, and nocturia. More serious complications of BPH include urinary retention, gross hematuria, bladder calculi, recurrent urinary tract infection, obstructive uropathy, and renal failure. Evaluation of BPH includes a detailed history, objective assessment of urinary symptoms with validated questionnaires, and measurement of bladder function parameters, including uroflowmetry and postvoid residual. In general, treatment of LUTS associated with BPH is based on the effect of the symptoms on quality of life (QOL) and include medical therapy aimed at reducing outlet obstruction or decreasing the size of the prostate. If medical therapy fails or is contraindicated, various surgical options exist. As the elderly population continues to grow, the management of BPH will become more common and important in maintaining patient's QOL.

  8. Carvedilol for the treatment of benign prostatic hypertrophy in patients with heart failure?

    Science.gov (United States)

    Rohrer, Colleen K; Page, Robert Lee; Shakar, Simon F; Lindenfeld, Joann

    2011-10-01

    Heart failure (HF) and benign prostatic hypertrophy (BPH) are two conditions that commonly coexist in men 60 years and older. Carvedilol is the only β-adrenergic blocker approved for HF that also has additional α1-adrenergic blockade. As α1-adrenergic blockers are used in the treatment of BPH, it is intuitive that carvediolol could improve BPH symptoms. We present a case where carvedilol was replaced with bisoprolol resulting in acute urinary retention. When carvediolol was reinstituted, the patient's symptoms of BPH resolved. Benign prostatic hypertrophy was later diagnosed by digital rectal exam. Six month after reinstituting the carvediolol, the patient remains free of his BPH symptoms. This case suggests that carvedilol may be considered for the management of HF with systolic dysfunction in patients with concomitant BPH thus eliminating the need for an α1-adrenergic blockers.

  9. Expression of hypoxia-inducible factor 1α in human normal, benign, and malignant prostate tissue

    Institute of Scientific and Technical Information of China (English)

    都镇先; 藤山千里; 陈永昕; 真崎善二郎

    2003-01-01

    Objective To investigate hypoxia-inducible factor 1α (HIF-1α) protein expression in normal prostates (NP), benign prostatic glandular hyperplasia (BPH), and prostate adenocarcinoma (Pca).Methods HIF-1α protein expression was determined by immunohistochemistry in formalin-fixed and paraffin-embedded specimens obtained from 13 cases of NP, 28 cases of BPH, and 34 cases of Pca. In cases of Pca, the relationship between HIF-1α protein expression and certain clinicopathological factors, such as clinicopathologic stage and Gleason score, was evaluated.Results NP manifested no immunoreactivity, whereas Pca and BPH showed significantly increased HIF-1α protein expression. A significantly higher expression was observed in Pca specimens compared with BPH samples. In Pca, no significant relationship between HIF-1α protein expression and clinicopathological factors was found.Conclusion Our findings of increased HIF-1α protein expression in BPH and Pca specimens suggests the potential role of this protein in BPH and Pca.

  10. Resistance of transgenic rice pure lines to brown planthopper

    Institute of Scientific and Technical Information of China (English)

    2001-01-01

    @@Brown planthopper (Nilaparvata lugens, BPH) is one of the most damaging rice insect pests. Recent studies showed that lectin (GNA), coded by the gna gene from snowdrop (Galanthus nivalis) was toxic to BPH in artificial diet assay (Powell et al, 1993, 1995). Here we report the development of homozygous transgenic rice lines contained the gna gene and the BPH bioassay test of the homozygous lines.

  11. Pathological and morphological features of bladder neck sclerosis

    OpenAIRE

    Klochay V.V.; Krupin V.N.; Nashivochnikova N.A.; Artifeksova A.A.

    2011-01-01

    The research goal is to estimate a condition of microcirculation in a bladder neck in patients with benign prostatic hyperplasia (BPH) and its value in pathogenesis of bladder neck sclerosis. Materials and methods: Work basis included the analysis of treatment results of 1171 patients with BPH who carried complex examination, operative treatment and further supervision. The basic group was made up of 182 patients with BPH who developed bladder neck sclerosis in the postoperative period. The c...

  12. Recent advances in treatment for Benign Prostatic Hyperplasia [version 1; referees: 2 approved

    Directory of Open Access Journals (Sweden)

    Simon van Rij

    2015-12-01

    Full Text Available Clinical benign prostatic hyperplasia (BPH, often identified as a worsening ability of a male to pass urine, is a significant problem for men in our society. In 2015, the use of personalised medicine is tailoring treatment to individual patient needs and to genetic characteristics. Technological advances in surgical treatment are changing the way BPH is treated and are resulting in less morbidity. The future of BPH treatments is exciting, and a number of novel techniques are currently under clinical trial.

  13. Incidence, Predictors, and Associated Outcomes of Prostatism after Kidney Transplantation

    OpenAIRE

    2009-01-01

    Background and objectives: Renal transplantation is increasingly performed in elderly patients, and the incidence of benign prostatic hyperplasia (BPH) increases with age. Anuric males on dialysis may have occult BPH and not develop obstructive symptoms until urine flow is restored after transplantation. If left untreated, BPH poses a risk for numerous complications, including acute urinary retention (AUR), recurrent urinary tract infections (UTI), and renal failure. The authors hypothesized ...

  14. Relationship of blood pressure variability and heart rate variability with prostatic volume in patients with benign prostatic hyperplasia

    Institute of Scientific and Technical Information of China (English)

    金江丽

    2014-01-01

    Objective To investigate the relationship of blood pressure variability(BPV)and heart rate variability(HRV)with prostatic volume(PV)in patients with benign prostatic hyperplasia(BPH).Methods A total of133 patients admitted to our department between January2011 and April 2013 were analyzed retrospectively.The patients were divided into BPH group and non-BPH group according to the PV value.The ambulatory blood

  15. Withania coagulans Extract Induces Cell Apoptosis and Inhibits COX-2 Expression in a Rat Model of Benign Prostatic Hyperplasia

    Directory of Open Access Journals (Sweden)

    Sarbishegi

    2016-08-01

    Full Text Available Background Phytotherapy is a popular treatment option in cases of benign prostatic hyperplasia (BPH, with many different herbal products being used for the treatment of this condition. Withania coagulans (WC is an herbal medicine that has shown anti-tumoral, anti-inflammatory, and antioxidant effects. Objectives This study examined the effect of Withania coagulans extract (WCE on prostatic cell apoptosis and cyclooxygenase-2 (COX-2 expression in cases of benign prostatic hyperplasia (BPH in rats. Methods Forty Wistar rats were equally divided into five groups: control, sham, BPH, BPH + WCE, and BPH + CLX (celecoxib as a positive control group. The induction of BPH was achieved via the subcutaneous injection of 3 mg/kg of testosterone propionate (TP daily for 28 days. The animals received WCE, celecoxib, or distilled water by oral gavage accompanied by the TP injection. After four weeks, the prostate glands of the rats were weighed to measure the prostatic index (PI. The ventral lobes of the prostates were dissected and processed with paraffin blocks in order to study the number of mast cells. A TUNEL analysis was performed to evaluate the cell apoptosis, while the expression of COX-2 was examined using immunohistochemistry. Results BPH was obvious in the ventral lobe of the prostate, and the administration of WCE markedly decreased the PI and the number of mast cells (P < 0.001 in the BPH rats. Additionally, the WCE treatment induced prostatic cell apoptosis when compared to the BPH group. Furthermore, following the WCE treatment, the expression of COX-2 in the prostatic tissues was significantly decreased when compared to the BPH groups. Conclusions According to the results of this study, WCE was effective in the treatment of BPH in rats. It may therefore have beneficial effects in the treatment of patients with BPH.

  16. Ursolic acid reduces prostate size and dihydrotestosterone level in a rat model of benign prostatic hyperplasia.

    Science.gov (United States)

    Shin, In-Sik; Lee, Mee-Young; Jung, Da-Young; Seo, Chang-Seob; Ha, Hye-Kyung; Shin, Hyeun-Kyoo

    2012-03-01

    Benign prostatic hyperplasia (BPH) is characterized by hyperplasia of prostatic stromal and epithelial cells, which can lead to lower urinary tract symptoms. The prevalence of BPH increases in an age-dependent manner. We investigated the protective effect of ursolic acid in BPH development using a testosterone-induced BPH rat model. BPH was induced in experimental groups by daily subcutaneous injections of testosterone propionate (TP), for a period of four weeks. Ursolic acid was administrated daily by oral gavage at a dose level of 5mg/kg during the four weeks of TP injections. Animals were sacrificed on the scheduled termination, before prostates were weighed and subjected to histopathological examination. TP and dihydrotestosterone (DHT) levels in the serum and prostate were also measured. BPH-induced animals displayed an increase in prostate weight with increased testosterone and DHT levels in both the serum and prostate. However, ursolic acid treatment resulted in significant reductions in prostate weight and testosterone and DHT levels in both the serum and prostate, compared with BPH-induced animals. Histopathological examination also showed that ursolic acid treatment suppressed TP-induced prostatic hyperplasia. These findings indicate that ursolic acid may effectively inhibit the development of BPH and it may be a useful agent in BPH treatment.

  17. Oxybutynin

    Science.gov (United States)

    ... a disorder of the nervous system that causes muscle weakness); fast or irregular heartbeat; high blood pressure; benign prostatic hypertrophy (BPH, enlargement of the prostate, a male reproductive ...

  18. Effects of herbivore-induced rice volatiles on the host selection behavior of brown planthopper, Nilaparvata lugens

    Institute of Scientific and Technical Information of China (English)

    2002-01-01

    It has been suggested that herbivore would react to volatiles produced by herbivore infested plant due to potential change, either positive or negative, in the acceptability of the host plant. This hypothesis was tested for the brown planthopper (BPH) in the laboratory. Sixteen components of the headspace volatiles from rice seedlings with different treatments were collected with SPME and Tenax-TA trap and analyzed with GC and GC-MS. Significant differences in volatile emissions were observed for rice plants with different treatments. Undamaged control plants, mechanically damaged plants and the plants infested by BPH for 1 or 2 d emitted much lower amounts of volatiles compared to the plants infested by BPH for 3 or 5 d. The plants infested by BPH for 3 or 5 d emitted several volatiles that were not detected in undamaged control plants, mechanically damaged plants or the plants infested by BPH for 1 or 2 d. Spodoptera litura infested plants released much higher amounts of volatiles than those in all other treatments, and the contents of several green leaf volatiles, methyl salicylate and terpenoids increased dramatically. In dual-choice flight tunnel experiments, adult BPH females showed no significant preference between the untreated healthy plants and mechanically damaged plants or the plants infested by BPH adult females. However, rice plants damaged by S. litura had a clearly repellent effects on BPH adult females compared to healthy undamaged plants, mechanically damaged plants or the plants infested by BPH.

  19. Mapping of two new brown planthopper resistance genes from wild rice

    Institute of Scientific and Technical Information of China (English)

    2001-01-01

    A brown planthopper (BPH) resistance line, B5, derived its resistance genes from the wild rice Oryza officinalis Wall exwatt, was hybridized with Taichung Native 1, a cultivar highly susceptible to BPH. A mapping population composed of randomly selected 167 F2 individuals was used for determining the BPH resistance genes by the restriction fragment length polymorphism analysis (RFLP). Bulked segregant analysis was conducted to identify RFLP makers linked to the BPH resistance genes in B5. The results indicat-ed that the markers linked to BPH resistance are located at two genomic regions on the long arm of chromosome 3 and the short arm of chromosome 4, respectively. The existence of the two loci was further assessed by the quantitative trait locus (QTL) analysis. We located the two loci at a 3.2 cM interval between G1318 and R1925 on chromosome 3 and a 1.2 cM interval between C820 and S11182 on chromosome 4. Comparison with the BPH genes that have been reported indicated that the BPH resistance genes in B5 are novel. These two genes may be useful BPH resistance resource for rice breeding. Furthermore, the mapping of the two genes is useful for cloning the BPH resistance genes.

  20. Structural properties of platinum(II) biphenyl complexes containing 1,10-phenanthroline derivatives

    Science.gov (United States)

    Rillema, D. Paul; Cruz, Arvin J.; Tasset, Brandon J.; Moore, Curtis; Siam, Khamis; Huang, Wei

    2013-06-01

    Seven platinum(II) complexes formulated as Pt(bph)L, where bph is the 2,2'-biphenyl dianion and L = 4-methyl-1,10-phenanthroline (4-Mephen), 5-methyl-1,10-phenanthroline (5-Mephen), 5-chloro-1,10-phenanthroline (5-Clphen), 5,6-dimethyl-1,10-phenanthroline (5,6-Me2phen), 4,7-dimethyl-1,10-phenanthroline (4,7-Me2phen), 4,7-diphenyl-1,10-phenanthroline (4,7-Ph2phen) and 3,4,7,8-tetramethyl-1,10-phenanthroline (3,4,7,8-Me4phen) are reported. Protons attached to the phen ligand resonate downfield from those attached to the bph ligand and two proton signals are split by interaction with 195Pt. Pt(bph)(3,4,7,8-Me4phen), Pt(bph)(4,7-Me2phen), Pt(bph)(5,6-Me2phen), Pt(bph)(4,7-Ph2phen) and Pt(bph)(5-Mephen) crystallize in the space groups Pna21, P21/n, P21/c, P - 1 and Pca21, respectively. The structures of the complexes deviate from true planarity and divide themselves into two groups where the bph and phen ligands cross in an X configuration or bow out in a butterfly (B) configuration. Circular dichroism revealed two different spectra with respect to the X and B configurations.

  1. 76 FR 6788 - Radio Broadcasting Services; AM or FM Proposals To Change The Community of License.

    Science.gov (United States)

    2011-02-08

    ... COMMISSION Radio Broadcasting Services; AM or FM Proposals To Change The Community of License. AGENCY... 3338, BPH- 20101222ABD, From MARLOW, OK, To LAWTON, OK; FISHER RADIO REGIONAL GROUP, INC., Station KQDI-FM, Facility ID 32386, BPH-20101222ABO, From GREAT FALLS, MT, To HIGHWOOD, MT; THE MONTANA...

  2. 78 FR 48625 - Radio Broadcasting Services; Centerville, Lovelady, Midway, and Oakwood, Texas

    Science.gov (United States)

    2013-08-09

    ... COMMISSION 47 CFR Part 73 Radio Broadcasting Services; Centerville, Lovelady, Midway, and Oakwood, Texas... applications (File Nos. BPH-20120529ADK and BPH-20120529ADI). List of Subjects in 47 CFR Part 73 Radio, Radio broadcasting. Federal Communications Commission. Nazifa Sawez, Assistant Chief, Audio Division, Media...

  3. Clinical effects of statins on benign prostatic hyperplasia complicating metabolic syndrome in elderly patients

    Institute of Scientific and Technical Information of China (English)

    曾小芳

    2014-01-01

    Objective To evaluate the effect of simvastatin and atorvastatin on clinical progression of benign prostatic hyperplasia(BPH)in elderly patients with metabolic syndrome(MS).Methods A total of 135 patients with BPH and MS aged 60 years and over were divided into three groups:simvastatin group(n=45,40 mg/d),atorvasta-

  4. LILRA3 Is Associated with Benign Prostatic Hyperplasia Risk in a Chinese Population

    Directory of Open Access Journals (Sweden)

    Jielin Sun

    2013-04-01

    Full Text Available A recent prostate cancer (PCa genome-wide association study (GWAS identified rs103294, a single nucleotide polymorphism (SNP located on LILRA3, a key component in the regulation of inflammatory inhibition, to be significantly associated with PCa risk in a Chinese population. Because inflammation may be a common etiological risk factor between PCa and benign prostatic hyperplasia (BPH, the current study was conducted to investigate the association of rs103294 with BPH risk. rs103294 was genotyped in a Chinese population of 426 BPH cases and 1,008 controls from Xinhua Hospital in Shanghai, China. Association between rs103294, BPH risk and clinicopathological traits were tested with adjustment for age. rs103294 was significantly associated with BPH risk with a p-value of 0.0067. Individuals with risk allele “C” had increased risk for BPH (OR = 1.34, 95% CI: 1.09–1.66. Stratified analysis revealed a stronger association risk for younger patients who are below 72 years old (OR = 1.51, 95% CI: 1.06–2.16. Our study represents the first effort to demonstrate that LILRA3 gene is significantly associated with BPH risk in a Chinese population. Our results support a common role of inflammation in the development of PCa and BPH. Additional studies are needed to further evaluate our results.

  5. Immunohistochemical analysis of estrogen receptors in prostate and clinical correlation in men with benign prostatic hyperplasia

    Science.gov (United States)

    Gangkak, Goto; Mittal, Alka; Yadav, Sher Singh; Tomar, Vinay; Yadav, Ajay; Mehta, Jayanti

    2017-01-01

    Purpose Estrogens act through interaction with 2 receptor subtypes, ER alpha (ERα) and ER beta (ERβ), in human prostate. The aim of the present study was to semiquantitatively assess the differential expression of ER subtypes in human benign prostatic hyperplasia (BPH) by use of immunocytochemistry (IHC) methods and to explore their relationship with various measures of BPH. Materials and Methods A total of 45 patients with BPH undergoing transurethral resection of the prostate and 22 patients with bladder cancer with normal prostate undergoing surveillance cystoscopy were studied as cases and controls, respectively. Quantitative immunolabeling of ER subtypes was scored by use of a semiquantitative scale. Also, correlations were assessed between ER levels in prostate and various measures of BPH. Results Overall, we found strong immunostaining for ERα in stroma and for ERβ in epithelium, respectively. The IHC score for ERα differed significantly between BPH patients and controls in both stroma (p≤0.001) and epithelium (p=0.008), respectively. The ERβ IHC score was also significantly higher in the epithelium of BPH patients (p=0.01). Also, we found a significant correlation between prostatic ER levels and various clinical measures of BPH. Conclusions ERs may play an important role in the pathogenesis of BPH.

  6. Validation of a patient reported outcome questionnaire for assessing success of endoscopic prostatectomy

    Directory of Open Access Journals (Sweden)

    Tania Hossack

    2014-12-01

    Conclusions: This is the first study to validate the PGI-I as a PRO measure to surgery for BOO. This suggests a potential for the PGI-I to be used to assess surgical therapies for BPH and may be a valuable addition for measuring outcomes in clinical trials evaluating surgical interventions for BPH.

  7. Incidence of benign prostate hypertrophy in Danish men with and without HIV infection

    DEFF Research Database (Denmark)

    Ahlström, Magnus Glindvad; Kronborg, Gitte; Larsen, Carsten S

    2015-01-01

    BACKGROUND: Information on risk of benign prostate hypertrophy (BPH) in HIV-infected men is sparse. We aimed to estimate the incidence of being diagnosed with BPH among HIV-infected men compared with an age and sex-matched comparison cohort from the background population. To exclude that family...

  8. [FEATURES OF CLINICAL COURSE AND MORPHOMETRIC PARAMETERS OF BENIGN PROSTATIC HYPERPLASIA IN MEN WITH METABOLIC SYNDROME AND ANDROGEN DEFICIENCY].

    Science.gov (United States)

    Tyuzikov, I A; Grekov, E A; Kalinchenko, S Yu

    2015-01-01

    The study was aimed to the evaluation of the effect of the components of the metabolic syndrome (obesity, insulin resistance (IR)) and androgen deficiency on the clinical course of lower urinary tract symptoms against the background of benign prostatic hyperplasia (LUTS/BPH) and nocturia, as well as on some of the parameters of BPH (prostate volume, residual urine volume, total prostate-specific antigen (PSA) blood level). The comprehensive survey of 160 men with LUTS/BPH (mean age 56.7 ± 3.3 years) was performed; based on the results of survey, three comparison groups were formed: Group 1 (n = 70)--patients with isolated obesity; Group 2 (n = 36)--patients with obesity and insulin resistance; and Group 3 (n = 54)--patients with obesity, insulin resistance and androgen deficiency. The control group consisted of 30 patients with LUTS/BPH without these metabolic and hormonal disorders. In patients with LUTS/BPH and obesity, higher frequency of nocturia compared with the control group was revealed (63.7% vs 23.3%; P resistance and androgen deficiency had the highest average prostate volume and residual urine volume compared with those of other groups (P resistance, androgen deficiency are associated pathological conditions, greatly aggravating the clinical course of LUTS/BPH due to adverse impact on the BPH parameters, acting both together and separately. The most severe LUTS/BPH were associated with the presence of all three of the above systemic disorders.

  9. Immunodetection of a brown planthopper (Nilaparvata lugens Stål) salivary catalase-like protein into tissues of rice, Oryza sativa.

    Science.gov (United States)

    Petrova, A; Smith, C M

    2014-02-01

    Saliva plays an important role in host plant-phloem-feeding insect molecular interactions. To better elucidate the role of insect saliva, a series of experiments were conducted to establish if catalase from the salivary glands of the brown planthopper (BPH; Nilaparvata lugens Stål) was secreted into rice host plant tissue during feeding. Catalase is the main enzyme that decomposes hydrogen peroxide (H2O2) at high concentrations. H2O2 is a part of the free radicals system that mediates important physiological roles including signalling and defence. Previous studies have suggested that H2O2 is involved in the rice endogenous response to BPH feeding. If, the BPH secretes catalase into host plant tissue this will counter the effects of H2O2, from detoxification to interfering with plant signalling and defence mechanisms. When BPHs were fed on a hopper-resistant rice variety for 24 h, catalase activity in the salivary glands increased 3.5-fold compared with hoppers fed on a susceptible rice variety. Further supporting evidence of the effects of BPH catalase was demonstrated by immunodetection analyses where results from two independent sources: BPH-infested rice tissue and BPH-probed artificial diets, suggest that the BPH secretes catalase-like protein during feeding. The possible physiological roles of BPH-secreted catalase are discussed.

  10. Increased Antioxidant Quality Versus Lower Quantity Of High Density Lipoprotein In Benign Prostatic Hyperplasia

    Directory of Open Access Journals (Sweden)

    Aydin Ozgur

    2015-10-01

    Full Text Available Background: Oxidative stress may be involved in the pathogenesis of every human disease. To understand its possible role in benign prostatic hyperplasia (BPH, we measured the overall oxidative status of patients with BPH and the serum activity of the high density lipoprotein (HDL-related antioxidant enzymes paraoxonase 1 (PON1 and arylesterase (ARE.

  11. Expression of indica rice resistant sources to Brown planthopper(BPH) in indica-japonica hybrids and their utillzation

    Institute of Scientific and Technical Information of China (English)

    YANGTibin; XUSuoshun; GUFulin

    1992-01-01

    We studied the genetic mode in transferring BPH-resistance genes from indica varieties to japonica varieties January 1988 to December 1989 in Nanjing, Jiangsu Province. Indica varieties selected on a basis of BPH-resistance genes, i.e., Yankeng 2 (japonica), 02428 (japonica), 40316 (indica-japonica progeny),

  12. Analysis and reliability of data from 24-hour frequency-volume charts in men with lower urinary tract symptoms due to benign prostatic hyperplasia

    NARCIS (Netherlands)

    Gisolf, KWH; van Venrooij, GEPM; Eckhardt, MD; Boon, TA

    2000-01-01

    Objectives: The aims of this study were to analyse the data from frequency-volume charts and to study the reliability of these charts in men with lower urinary tract symptoms (LUTS) due to benign prostatic hyperplasia (BPH), Methods: Males with LUTS due to BPH were consecutively included in the stud

  13. Diagnosis of prostate cancer by quantitative analysis of 3DMRSI data: A new model

    Institute of Scientific and Technical Information of China (English)

    QUAN Hong; WANG Xiaoying; BAO Shanglian; WANG Huiliang; LI Feiyu; HUANG Rong

    2005-01-01

    Three-dimensional magnetic resonance spectroscopic imaging (3DMRSI) is helpful to identify prostate cancer (PC)from benign prostate hyperplasia (BPH) and to show the distribution of tumor infiltration. Combined with the (Cho + Cre)/Cit ratio, the z-score model can effectively discriminate prostate cancer from stromal benign prostate hyperplasia (sBPH) and detect small malignant lesions (SML).

  14. Epidemiology and management of symptomatic benign prostatic hyperplasia

    NARCIS (Netherlands)

    K.M.C. Verhamme (Katia)

    2004-01-01

    textabstractBenign prostatic hyperplasia (BPH) is the most common non-cancerous form of cell growth in men and usually begins with the formation of microscopic nodules in younger men. As BPH progresses, overgrowth occurs in the central area of the prostate, called the transition zone, which wraps ar

  15. Association of a Common Variant at 10q26 and Benign Prostatic Hyperplasia Aggressiveness in Han Chinese Descent

    Directory of Open Access Journals (Sweden)

    Xin Gu

    2013-01-01

    Full Text Available Recent studies reported that rs2252004 at 10q26 was significantly associated with prostate cancer (PCa risk in a Japanese population and was subsequently confirmed in a Chinese population. We aimed to assess the relationship between this locus and risk/aggressiveness of benign prostatic hyperplasia (BPH. The current study included 426 BPH cases and 1,008 controls from Xinhua Hospital in Shanghai, China. All BPH patients were treated with α-adrenergic blockers and 5α-reductase inhibitors for at least 9 months. Associations between rs2252004 and BPH risk/aggressiveness were tested using logistic regression. Associations between rs2252004 and clinical parameters including International Prostate Symptom Score (IPSS, total prostate volume (TPV, total PSA (tPSA, and free PSA (fPSA were evaluated by linear regression. Allele “A” in rs2252004 was significantly associated with increased risk for aggressiveness of BPH in a Chinese population (OR = 1.42, 95% CI: 1.04–1.96, P=0.03. Patients with the genotype “A/A” (homozygous minor allele had an increase of IPSS and TPV after treatment (P=0.045 and 0.024, resp.. No association was observed between rs2252004, BPH risk, and baseline clinicopathological traits (All P>0.05. Our study is the first to show that rs2252004 at 10q26 was associated with BPH aggressiveness and efficacy of BPH treatment.

  16. Labda-8(17),12,14-trien-19-oic acid contained in fruits of Cupressus sempervirens suppresses benign prostatic hyperplasia in rat and in vitro human models through inhibition of androgen and STAT-3 signaling.

    Science.gov (United States)

    Verma, Vikas; Sharma, Vikas; Singh, Vishal; Kumar, Rajeev; Khan, Mohammad F; Singh, Anil K; Sharma, Rolee; Arya, Kamal R; Maikhuri, J P; Dalela, Diwakar; Maurya, Rakesh; Gupta, Gopal

    2014-08-01

    Fruit extract of Cupressus sempervirens (CS), which is used traditionally to treat Benign Prostatic Hyperplasia (BPH)-like urinary symptoms in patients, was scientifically validated for anti-BPH activity. The ethanolic fruit extract of CS inhibited proliferation of human BPH-stromal cells and the activity was localized to its chloroform-soluble, diterpene-rich fraction. Eight major diterpenes isolated from this fraction exhibited moderate to potent activity and the most active diterpene (labda-8(17),12,14-trien-19-oic acid) exhibited an IC50 of 37.5 μM (antiproliferative activity against human BPH-stromal cells). It significantly inhibited activation (phosphorylation) of Stat-3 in BPH-stromal cells and prevented transactivation of androgen sensitive KLK3/PSA and TMPRSS2 genes in LNCaP cells. Labda-8(17),12,14-trien-19-oic acid-rich CS fraction prevented prostatic hyperplasia in rat model and caused TUNEL labeling of stromal cells with lower expressions of IGF-I, TGF-ß and PCNA, and bcl-2/bax ratio. Human BPH tissues exhibited precise lowering of stromal component after incubation in labda-8(17),12,14-trien-19-oic acid, ex vivo. We conclude that labda-8(17),12,14-trien-19-oic acid contained in CS exhibits anti-BPH activity through inhibition of stromal proliferation and suppression of androgen action in the prostate, presenting a unique lead structure for further optimization of anti-BPH activity.

  17. Utility of Combination of Diagnostic Tests in Early Detection of Prostate Tumors in West Algerian Hospital

    Directory of Open Access Journals (Sweden)

    Abdelkrim Berroukche

    2014-09-01

    Full Text Available In West of Algeria, incidence of prostate cancer (PCa is growing and epidemiological data on benign prostatic hyperplasia (BPH are contradictory. The prostate specific antigen (PSA joins other tests, as digital rectal examination (DRE and ultrasound, for better management of patient. This study aimed to assess the association of previously tests with PSA-assay in diagnosis of PCa and BPH, in West Algeria. A retrospective study was performed on two groups of 234 BPH and 56 PCa diagnosed between 2010–2012 at the urology department of the hospital in Saida. Patients underwent various diagnostic tests; DRE, ultrasound, PSA and pathological examination. BPH is more common than PCa and the most dominant age group was from 70 to 79 years. DRE was positive in 67 % of BPH and 63.3 % PCa. Ultrasound examination revealed that the prostatic structure was heterogeneous in 25.5 % of BPH and 92 % PCa. Total PSA (TPSA was higher than the cut-off value of 4 ng / ml in 37% of BPH and 75 % PCa. Histological forms of Prostatic adenomyofibroma and prostatic adenocarcinoma were the most represented. This study shows that the combination of different tests is more efficient than using a test alone for a definitive diagnosis of PCa or BPH.

  18. Clinical value of prostate segmentation and volume determination on MRI in benign prostatic hyperplasia.

    Science.gov (United States)

    Garvey, Brian; Türkbey, Barış; Truong, Hong; Bernardo, Marcelino; Periaswamy, Senthil; Choyke, Peter L

    2014-01-01

    Benign prostatic hyperplasia (BPH) is a nonmalignant pathological enlargement of the prostate, which occurs primarily in the transitional zone. BPH is highly prevalent and is a major cause of lower urinary tract symptoms in aging males, although there is no direct relationship between prostate volume and symptom severity. The progression of BPH can be quantified by measuring the volumes of the whole prostate and its zones, based on image segmentation on magnetic resonance imaging. Prostate volume determination via segmentation is a useful measure for patients undergoing therapy for BPH. However, prostate segmentation is not widely used due to the excessive time required for even experts to manually map the margins of the prostate. Here, we review and compare new methods of prostate volume segmentation using both manual and automated methods, including the ellipsoid formula, manual planimetry, and semiautomated and fully automated segmentation approaches. We highlight the utility of prostate segmentation in the clinical context of assessing BPH.

  19. Expression analysis of thrombospondin 2 in prostate cancer and benign prostatic hyperplasia.

    Science.gov (United States)

    Matos, A R; Coutinho-Camillo, C M; Thuler, L C S; Fonseca, F P; Soares, F A; Silva, E A; Gimba, E R

    2013-06-01

    Thrombospondin 2 (TSP2) is a protein with important roles in different tumor types, mainly related to tumor inhibition. However, there are limiting data regarding TSP2 in prostate cancer (PCa) and benign prostatic hyperplasia (BPH). We aimed to investigate TSP2 transcript and protein expression in tumoral and non-tumoral prostate tissues and cell lines, and its implications for PCa diagnosis and progression. TSP2 transcript expression was evaluated by real time PCR in PCa and BPH tissue samples and in tumoral and non-tumoral cell lines. TSP2 protein expression analysis was conducted by immunohistochemistry in a tissue microarray (TMA) containing PCa and BPH tissue samples. TSP2 transcript was down-regulated in PCa tissue samples and cell lines, when compared to BPH and non-tumoral samples (PBPH tissue samples (PBPH epithelial and stromal compartments. TSP2 stromal staining scores were significantly lower in PCa than in BPH tissues (PBPH diagnosis.

  20. Association between Single Nucleotide Polymorphism of Vitamin D Receptor Gene FokI Polymorphism and Clinical Progress of Benign Prostatic Hyperplasia

    Directory of Open Access Journals (Sweden)

    Li Ruan

    2015-01-01

    Full Text Available Background. The aim of the study was to investigate the association between single nucleotide polymorphism (SNP of vitamin D receptor (VDR gene and clinical progress of benign prostatic hyperplasia (BPH in Chinese men. Methods. The DNA was extracted from blood of 200 BPH patients with operation (progression group and 200 patients without operation (control group, respectively. The genotypes of VDR gene FokI SNP represented by “F/f” were identified by PCR-restriction fragment length polymorphism. The odds ratio (OR of having progression of BPH for having the genotype were calculated. Results. Our date indicated that the f alleles of the VDR gene FokI SNP associated with the progression of BPH (P=0.009. Conclusion. For the first time, our study demonstrated that VDR gene FokI SNP may be associated with the risk of BPH progress.

  1. Benign prostate hyperplasia and stem cells: a new therapeutic opportunity.

    Science.gov (United States)

    Notara, Maria; Ahmed, Aamir

    2012-12-01

    Most men over 50 experience some lower urinary tract symptoms of nocturia, poor stream, urgency and frequency for urination, due to hyperplastic enlargement of the prostate (benign prostate hyperplasia, BPH). BPH is thought to be a disease with multiple aetiologies including hormone signalling, disruption of proliferation and apoptosis dynamics and chronic inflammation with changes in the morphology and phenotype of the prostate stroma. It has been proposed, recently, that stromal stem cells in prostate may be caused by the development of BPH. This review focuses on this putative role of stromal stem or stem-like cells in the development of BPH and assesses the potential of targeting the stem cells for the treatment of BPH.

  2. 四苯硼钠在活性炭表面吸附过程的疏水相互作用%Hydrophobic interaction for the adsorption of sodium tetraphenylborate onto activated carbon

    Institute of Scientific and Technical Information of China (English)

    于玲; 王晓玲; 邹立壮; 支献华; 朱书全

    2003-01-01

    In this paper, the adsorption characteristics of sodium tetraphenylborate(NaBPh4) on activated carbon at 298.2,303.2,308.2,313..2 and 323.2 K was studied.The results show that the adsorption isotherm of NaBPh4 on activated carbon at different temperatures could be described using Langrnuir equation. Furthermore, the standard Gibbs energy, enthalpy, entropy and hydrophobic interaction Gibbs energy for the adsorption of NaBPh4 on activated carbon were studied, and the result shows that the hydrophobic interaction of BPh4- ion plays the most important role for the transfer of NaBPh4 from water to activated carbon surface.

  3. Malondialdehyde in benign prostate hypertrophy: a useful marker?

    Directory of Open Access Journals (Sweden)

    Rosaria Alba Merendino

    2003-01-01

    Full Text Available Benign prostate hypertrophy (BPH is the most common benign tumor in men due to obstruction of the urethra and, finally, uremia. Malondialdehyde (MDA is a product derived from peroxidation of polyunsaturated fatty acids and related esters. Evaluation of MDA in serum represents a non-invasive biomarker of oxidative stress. Prostate-specific antigen (PSA is a sensitive marker for prostatic hypertrophy and cancer. We analyzed MDA serum levels to evaluate the oxidative stress in BPH. To this end, 22 BPH patients and 22 healthy donors were enrolled. Data show an increase of MDA level in BPH patients and a positive correlation between PSA and MDA levels. In conclusion, we describe a previously unknown relationship between PSA and MDA as an index of inflammation and oxidative stress in BPH.

  4. The Association Between Metabolic Syndrome and Characteristics of Benign Prostatic Hyperplasia: A Systematic Review and Meta-Analysis.

    Science.gov (United States)

    Wang, Jian-Ye; Fu, Yan-Yan; Kang, De-Ying

    2016-05-01

    The purpose of this systematic review was to examine the association of metabolic syndrome (MS) with measures of benign prostatic hyperplasia (BPH) including prostate growth rate, prostate volume, International Prostate Symptom Score (IPSS), prostate-specific antigen (PSA) level, and maximal flow rate.Medline, Cochrane CENTRAL, EMBASE, CBM, and Google Scholar databases were searched until March 23, 2015 using combinations of the keywords benign prostate hyperplasia/BPH, metabolic syndrome, total prostate volume, prostate growth rate, prostate specific antigen, International Prostate Symptom Score/IPSS, maximal flow rate. Cohort or case-control studies of patients with BPH and MS that reported quantitative outcomes were included. The pooled mean differences of the outcome measures were compared between patients with and without MS.A total of 158 potentially relevant studies were identified, and 8 were included in the meta-analysis. The 8 studies included in the meta-analysis contained a total of 3093 BPH patients, wherein 1241 had MS and 1852 did not have MS. BPH patients with MS had a significantly higher prostate growth rate (pooled mean difference = 0.67 mL/y, P flow rate (pooled mean difference = -1.41 mL/s, P = .345) between BPH patients with and without MS. A borderline nonsignificant difference in PSA (pooled mean difference = 0.24 ng/mL, P = 0.056) was noted between BPH patients with and without MS.The results of this meta-analysis are consistent with literature indicating that BPH patients with MS have a higher prostate growth rate and larger prostate volume than those without MS; however, further study is necessary to determine the association of BPH and metabolic disorder elements and the potential risk of disease progression in BPH patients with MS.

  5. Survivin and NAIP in Human Benign Prostatic Hyperplasia: Protective Role of the Association of Serenoa repens, Lycopene and Selenium from the Randomized Clinical Study

    Science.gov (United States)

    Morgia, Giuseppe; Micali, Antonio; Rinaldi, Mariagrazia; Irrera, Natasha; Marini, Herbert; Puzzolo, Domenico; Pisani, Antonina; Privitera, Salvatore; Russo, Giorgio I.; Cimino, Sebastiano; Ieni, Antonio; Trichilo, Vincenzo; Altavilla, Domenica; Squadrito, Francesco; Minutoli, Letteria

    2017-01-01

    Benign prostatic hyperplasia (BPH) treatment includes the apoptosis machinery modulation through the direct inhibition of caspase cascade. We previously demonstrated that Serenoa repens (Ser) with lycopene (Ly) and selenium (Se) reawakened apoptosis by reducing survivin and neuronal apoptosis inhibitory protein (NAIP) levels in rats. The aim of this study was to evaluate the effectiveness of Ser-Se-Ly association on survivin and NAIP expression in BPH patients. Ninety patients with lower urinary tract symptoms (LUTS) due to clinical BPH were included in this randomized, double-blind, placebo-controlled trial. Participants were randomly assigned to receive placebo (Group BPH + placebo, n = 45) or Ser-Se-Ly association (Group BPH + Ser-Se-Ly; n = 45) for 3 months. At time 0, all patients underwent prostatic biopsies. After 3 months of treatment, they underwent prostatic re-biopsy and specimens were collected for molecular, morphological, and immunohistochemical analysis. After 3 months, survivin and NAIP were significantly decreased, while caspase-3 was significantly increased in BPH patients treated with Ser-Se-Ly when compared with the other group. In BPH patients treated with Ser-Se-Ly for 3 months, the glandular epithelium was formed by a single layer of cuboidal cells. PSA showed high immunoexpression in all BPH patients and a focal positivity in Ser-Se-Ly treated patients after 3 months. Evident prostate specific membrane antigen (PSMA) immunoexpression was shown in all BPH patients, while no positivity was present after Ser-Se-Ly administration. Ser-Se-Ly proved to be effective in promoting apoptosis in BPH patients. PMID:28327526

  6. Survivin and NAIP in Human Benign Prostatic Hyperplasia: Protective Role of the Association of Serenoa repens, Lycopene and Selenium from the Randomized Clinical Study

    Directory of Open Access Journals (Sweden)

    Giuseppe Morgia

    2017-03-01

    Full Text Available Benign prostatic hyperplasia (BPH treatment includes the apoptosis machinery modulation through the direct inhibition of caspase cascade. We previously demonstrated that Serenoa repens (Ser with lycopene (Ly and selenium (Se reawakened apoptosis by reducing survivin and neuronal apoptosis inhibitory protein (NAIP levels in rats. The aim of this study was to evaluate the effectiveness of Ser-Se-Ly association on survivin and NAIP expression in BPH patients. Ninety patients with lower urinary tract symptoms (LUTS due to clinical BPH were included in this randomized, double-blind, placebo-controlled trial. Participants were randomly assigned to receive placebo (Group BPH + placebo, n = 45 or Ser-Se-Ly association (Group BPH + Ser-Se-Ly; n = 45 for 3 months. At time 0, all patients underwent prostatic biopsies. After 3 months of treatment, they underwent prostatic re-biopsy and specimens were collected for molecular, morphological, and immunohistochemical analysis. After 3 months, survivin and NAIP were significantly decreased, while caspase-3 was significantly increased in BPH patients treated with Ser-Se-Ly when compared with the other group. In BPH patients treated with Ser-Se-Ly for 3 months, the glandular epithelium was formed by a single layer of cuboidal cells. PSA showed high immunoexpression in all BPH patients and a focal positivity in Ser-Se-Ly treated patients after 3 months. Evident prostate specific membrane antigen (PSMA immunoexpression was shown in all BPH patients, while no positivity was present after Ser-Se-Ly administration. Ser-Se-Ly proved to be effective in promoting apoptosis in BPH patients.

  7. Characterization of fibrillar collagens and extracellular matrix of glandular benign prostatic hyperplasia nodules.

    Directory of Open Access Journals (Sweden)

    Tyler M Bauman

    Full Text Available Recent studies have associated lower urinary tract symptoms (LUTS in men with prostatic fibrosis, but a definitive link between collagen deposition and LUTS has yet to be demonstrated. The objective of this study was to evaluate ECM and collagen content within normal glandular prostate tissue and glandular BPH, and to evaluate the association of clinical parameters of LUTS with collagen content.Fibrillar collagen and ECM content was assessed in normal prostate (48 patients and glandular BPH nodules (24 patients using Masson's trichrome stain and Picrosirius red stain. Second harmonic generation (SHG imaging was used to evaluate collagen content. Additional BPH tissues (n = 47 were stained with Picrosirius red and the association between clinical parameters of BPH/LUTS and collagen content was assessed.ECM was similar in normal prostate and BPH (p = 0.44. Total collagen content between normal prostate and glandular BPH was similar (p = 0.27, but a significant increase in thicker collagen bundles was observed in BPH (p = 0.045. Using SHG imaging, collagen content in BPH (mean intensity = 62.52; SEM = 2.74 was significantly higher than in normal prostate (51.77±3.49; p = 0.02. Total collagen content was not associated with treatment with finasteride (p = 0.47 or α-blockers (p = 0.52, pre-TURP AUA symptom index (p = 0.90, prostate-specific antigen (p = 0.86, post-void residual (PVR; p = 0.32, prostate size (p = 0.21, or post-TURP PVR (p = 0.51. Collagen content was not associated with patient age in patients with BPH, however as men aged normal prostatic tissue had a decreased proportion of thick collagen bundles.The proportion of larger bundles of collagen, but not total collagen, is increased in BPH nodules, suggesting that these large fibers may play a role in BPH/LUTS. Total collagen content is independent of clinical parameters of BPH and LUTS. If fibrosis and overall ECM deposition are

  8. Antiproliferative and Antioxidant Effects of Withania coagulans Extract on Benign Prostatic Hyperplasia in Rats

    Directory of Open Access Journals (Sweden)

    Sarbishegi

    2016-01-01

    Full Text Available Background Benign prostate hyperplasia (BPH is a common urological disorder in elderly men. Phytotherapy is frequently used to alleviate the symptoms of this condition. Objectives The present study investigated the effect of Withania coagulans extract (WCE, which is known to have antioxidant, anti-inflammatory, antihyperglycemic, and anti-cancer properties, on testosterone-induced BPH in rats. Materials and Methods Forty Wistar rats were divided into five groups (each n = 8: the control group, the untreated BPH group, and three WCE-treated groups (WCE250, 500, and 1000. BPH was induced with 3 mg/kg subcutaneous injections of testosterone propionate for four weeks. WCE was concomitantly administrated by oral gavage. At the end of the induction schedule, the animals were sacrificed and their prostate glands were dissected, weighed, and fixed for histological examination (H&E and proliferating cell nuclear antigen [PCNA] staining. Half of each sample was prepared for measurement of malondialdehyde (MDA and total antioxidant capacity (TAC levels in the prostate. Results The present study revealed that BPH caused elevation of MDA levels, suppression of TAC levels, and increased PCNA expression in the prostate gland. Interestingly, in a dose-dependent manner, WCE caused decreased MDA levels and increased TAC levels in the prostate gland, compared to the untreated BPH group. Histopathological examinations showed a reduction in PCNA expression in the prostate epithelium of the WCE animals. Conclusions W. coagulans inhibits the development of BPH can be useful for the treatment of this condition.

  9. Evolution of low-light adapted peripheral light-harvesting complexes in strains of Rhodopseudomonas palustris.

    Science.gov (United States)

    Kotecha, Abhay; Georgiou, Theonie; Papiz, Miroslav Z

    2013-03-01

    Purple bacteria have peripheral light-harvesting (PLH) complexes adapted to high-light (LH2) and low-light (LH3, LH4) growth conditions. The latter two have only been fully characterised in Rhodopseudomonas acidophila 7050 and Rhodopseudomonas palustris CGA009, respectively. It is known that LH4 complexes are expressed under the control of two light sensing bacteriophytochromes (BphPs). Recent genomic sequencing of a number of Rps. palustris strains has provided extensive information on PLH genes. We show that both LH3 and LH4 complexes are present in Rps. palustris and have evolved in the same operon controlled by the two adjacent BphPs. Two rare marker genes indicate that a gene cluster CL2, containing LH2 genes and the BphP RpBphP4, was internally transferred within the genome to form a new operon CL1. In CL1, RpBphP4 underwent gene duplication to RpBphP2 and RpBphP3, which evolved to sense light intensity rather than spectral red/far-red intensity ratio. We show that a second LH2 complex was acquired in CL1 belonging to a different PLH clade and these two PLH complexes co-evolved together into LH3 or LH4 complexes. The near-infrared spectra provide additional support for our conclusions on the evolution of PLH complexes based on genomic data.

  10. Applications of DNA-Markers to Analyze Rice Planthopper Resistance Genes%应用DNA标记分析稻飞虱的抗性基因

    Institute of Scientific and Technical Information of China (English)

    寒川一成; 曾娟; 钱忠海

    2003-01-01

    Recent achievements in molecular tagging and mapping of genetic loci for rice planthopper resistance in rice were briefly reviewed. Four rice genes for the brown planthopper (BPH) resistance, Bph 1, bph 2, bph 4 and Bph 9, and four putative BPH-resistance genes, Bph 10(t), bph 11(t), bph 12(t) and Bph 13(t), introgressed from wild rice species with different genomes have so far been mapped onto 5 of 12 rice chromosomes. Of them, Bph 1, bph 2, Bph 9 and Bph 10(t), have been found forming a linkage block on the long arm of rice chromosome 12, in the vicinity of about 25 cM from the bph 2 locus. Several QTLs affecting field resistance and ovicidal activities have also been detected. The whitebacked planthopper (WBPH) resistance genes, Wbph 1, Wbph 2 and Wbph 6(t) have been tagged or tentatively mapped. QTLs for ovicidal resistance to WBPH in japonica rice have been analyzed in detail. The effective QTL has been detected on the short arm of chromosome 6, and a dominant ovicidal gene Ovc has been identified at the locus. One QTL on chromosome 1 and two QTLs on chromosome 5 increased WBPH egg mortality in the presence of ovicidal gene Ovc. QTL mapping with DNA-markers will increase our understandings of complicated physiological and genetic mechanisms in varietal resistance in crop plants. Marker-assisted selection will facilitate to develop insect resistant crops with polygenic basis, and to introduce valuable insect resistance traits from wild relatives into improved crop varieties in order to increase durability and genetic diversity of insect resistance in the crops.%简要地回顾了水稻抗飞虱的遗传位点定位和作图的新进展.来自具有不同基因组的野生稻渗入系的4个抗褐飞虱基因Bph 1、 bph 2、 bph 4和Bph 9,以及4个暂定名抗褐飞虱基因Bph 10(t)、bph 11(t)、bph 12(t)和Bph 13(t),目前已被定位于水稻12条染色体中的5条.其中,Bph 1、 bph 2、 Bph 9和Bph 10(t)在水稻第12染色体的长臂上形成1个连锁区段,位于bph

  11. Pyramiding blast, bacterial blight and brown planthopper resistance genes in rice restorer lines

    Institute of Scientific and Technical Information of China (English)

    JI Zhi-juan; Yang Shu-dong; ZENG Yu-xiang; LIANG Yan; YANG Chang-deng; QIAN Qian

    2016-01-01

    Rice blast, bacterial blight (BB) and brown planthopper (BPH) are the three main pests of rice. This study investigated pyr-amiding genes resistant to blast, BB and BPH to develop restorer lines. Ten new lines with blast, BB and/or BPH resistance genes were developed using marker-assisted selection (MAS) technique and agronomic trait selection (ATS) method. Only HR13 with resistance genes to blast, BB and BPH was obtained. In addition to blast and BB resistance, four lines (HR39, HR41, HR42, HR43) demonstrated moderate resistance to BPH, but MAS for BPH resistance genes were not conducted in developing these four lines. These data suggested that there were unknown elite BPH resistance genes in the Zhongzu 14 donor parent. A more effective defense was demonstrated in the lines withPi1 andPi2 genes although the weather in 2012 was favorable to disease incidence. Blast resistance of the lines with a single resistance gene,Pita, was easily inlfuenced by the weather. Overal, the information obtained through pyramiding multiple resistance genes on developing the restorer lines is helpful for rice resistance breeding.

  12. Computed Tomography of the Prostate Gland in Healthy Intact Dogs and Dogs with Benign Prostatic Hyperplasia.

    Science.gov (United States)

    Pasikowska, J; Hebel, M; Niżański, W; Nowak, M

    2015-10-01

    To date, there is only scarce data on the evaluation of the prostate gland in dogs using computed tomography (CT). The aims of our study were to describe CT features of BPH in dogs and to determine the size of the prostate gland in healthy male dogs and dogs with benign prostatic hyperplasia (BPH) through CT. Additionally, we aimed to compare and establish the most useful parameters for CT measurements of the prostate in patients with BPH. The study population consisted of 20 healthy intact male dogs and 20 male intact dogs with confirmed BPH. Pre- and post-contrast CT studies were evaluated. The most common CT features in dogs with recognized BPH were symmetrical prostatomegaly and heterogeneity of the prostatic parenchyma. The mean prostatic density (D) was 56HU (±4.39) in pre-contrast CT images and 84HU (±8) in post-contrast images in dogs with BPH. The mean prostatic length (L) was 43.87 mm (±11), the mean width (W) amounted to 48.95 mm (±8.76) and the mean height (H) reached 44.9 mm (±9.48) in clinically affected patients. The mean ratios were: rL - 2,12 (±0.5); rW - 2.39 (±0.53) and rH - 2.16 (±0.39) in the BPH group. The prostate should be considered to be enlarged when rL exceeds 3.05; rW exceeds 3.38 and rH exceeds 2.94. Our findings indicated that CT is a useful tool in diagnosing prostate disorders, including BPH. The heterogeneity, density and ratios of prostatic length, width and height can be useful parameters in the diagnosis of BPH.

  13. Effects of Melandrium firmum methanolic extract on testosterone-induced benign prostatic hyperplasia in Wistar rats

    Institute of Scientific and Technical Information of China (English)

    Mee-Young Lee; In-Sik Shin; Chang-Seob Seo; Nam-Hun Lee; Hye-Kyung Ha; Jong-Keun Son; Hyeun-Kyoo Shin

    2012-01-01

    Benign prostatic hyperplasia (BPH) is an age-related disease of unknown aetiology characterized by prostatic enlargement coincident with distinct alterations in tissue histomorphology.Instead of therapeutic agents that can cause severe side effects,plant extracts are frequently used to treat BPH.In this study,we investigated whether the Melandrium firmum methanolic extract (M FM E) improves BPH,using the testosterone propionate (TP)-induced BPH rat model.Castration was performed via the scrotal route under sodium pentobarbital anaesthesia.BPH in castrated rats was generated via daily subcutaneous injections of TP (3 mg kg-1) dissolved in corn oil,for 4 weeks.MFME was administered daily by oral gavage at a dose of 200 mg kg-1 for 4 weeks,along with the TP injections.The control group received injections of corn oil subcutaneously.At the scheduled termination of the experiment,all rats were killed and their prostates weighed; the relative prostate weight (prostate/body weight ratio) was calculated,and histomorphological changes in the prostate were examined.Additionally,we measured the levels of testosterone and dihydrotestosterone (DHT) in the serum and the prostate.Experimentally induced BPH led to marked decreases in the relative prostate weight and the DHT levels in the serum and the prostate.Histologically,BPH was evident in the ventral lobe of the prostate,and MFME treatment suppressed the severity of the lesions.These results indicate that MFME effectively inhibits the development of BPH induced by testosterone in a rat model.Further studies will be needed to identify the compound(s) responsibility for inducing the protective effect against BPH and determine its mechanism of action.

  14. Effect of β Radiation on Bcl-2 and Bax Expressions in Benign Prostate Hyperplasia Tissues

    Institute of Scientific and Technical Information of China (English)

    MA Qing-jie; GAO Shi; ZHAO Jie; GU Xin-quan; CAI Shan-yu; ZHAO Guo-qing

    2008-01-01

    The authors chose specimens from nine normal prostate tissues(NP group),15 benign prostate hyperplasia(BPH) prostates(BPH group),and 35 BPH prostates that had been treated with 90Sr/90Y Prostatic Hyperplasia Applicator(exposure group),The expressions of bcl-2 and bax in stroma and epithelia of prostate tissues were demonstrated by means of immunohistochemical staining,and the staining positive rate was semiquantatively determined,so as to observe the expression of bcl-2 and bax genes in the prostate tissues of normal individuals and BPH patients,before and after β radiation,and to evaluate the influence of β radiation on bcl-2 and bax expressions,The expressions of gene bcl-2 in the prostate epithelia of NP and BPH are significantly higher than those in the prostate stroma(P<0.01),However,the expressions of bcl-2 in the prostate epithelia and stroma of the BPH group are obviously higher than those in the NP group(P<0.01),The expression of gene bax in the prostate epithelia of the NP group is higher than that in the BPH group(P<0.05),However,bcl-2 expressions in the prostate epithelia and stroma of the BPH group are significantly higher than the bax expressions(P<0.01),Compared with those of the NP group,the expressions of bcl-2 in the prostate epithelia and stroma of the exposure group decrease remarkably,even as the expressions of the bax notably increase(P<0.01),Thus,the administration of β radiation can remarkably affect bcl-2 and bax gene expressions,to regulate cell apoptosis,in the prostate tissues of BPH.

  15. Dynamic contrast-enhanced MRI in diagnosis of central gland prostate cancer and benign prostatic hyperplasia%动态增强MRI诊断前列腺中央腺体癌和不同类型前列腺增生

    Institute of Scientific and Technical Information of China (English)

    李鹏; 杨文君; 陈志强; 郭玉林; 刘云; 杨文静; 李燕; 杜奕

    2013-01-01

    目的 探讨3.0T LAVA多期动态增强扫描对前列腺中央腺体癌(CGPCa)和不同类型前列腺增生(BPH)的诊断价值.方法 回顾性分析经病理证实的34例CGPCa(CGPCa组)和70例BPH(BPH组)患者的MRI、动态增强MRI(DCE-MRI)资料,分析T2WI和动态增强表现,将信号强度-时间(SI-T)曲线分为速升下降型、速升平台型、缓升平台型和持续缓升型,并对前3种曲线计算达峰时间(Tmax)、最大强化程度(SImax)和最快强化率(Rmax),比较两组患者SI-T曲线类型及Tmax、SImax、Rmax的差异.结果 CGPCa组SI-T曲线以速升下降型和速升平台型为主;BPH组中基质增生为主型(sBPH)SI-T曲线类型与CGPCa类似;腺体增生为主型(gBPH)以持续缓升型多见;混合型增生(mBPH)四种曲线均可见,但以持续缓升型最多.CGPCa组、sBPH亚组、mBPH亚组、gBPH亚组间SI-T曲线类型的差异有统计学意义(P<0.01),但CGPCa组与sBPH亚组的差异无统计学意义(P>0.05),CGPCa组与gBPH亚组、mBPH亚组的差异均有统计学意义(P均<0.01).CGPCa组、sBPH亚组和mBPH亚组间Tmax、SImax、Rmax的差异均有统计学意义(P均<0.05),但CGPCa组和sBPH亚组间Tmax、SImax、Rmax存在较明显的重叠.结论 3.0T LAVA多期动态增强扫描SI-T曲线有助于CGPCa与gBPH、mBPH的鉴别诊断.Tmax、SImax、Rmax对CGPCa和sBPH、mBPH具有一定鉴别诊断价值.%Objective To explore the value of 3.0T liver acquisition with volume acceleration (LAVA) dynamic contrastenhanced MRI (DCE-MRI) in the diagnosis of central gland prostate cancer (CGPCa) and benign prostatic hyperplasia (BPH).Methods MRI and DCE-MRI data of 34 patients with CGPCa (CGPCa group) and 70 patients with BPH (BPH group) proved by pathology were analyzed retrospectively.By analyzing the characteristics of T2WI and DCE-MRI,the styles of the signal intensity-time curve (SI-T) were defined as rapidly ascending followed with descending curve,rapidly ascending followed with plateau

  16. Gclust Server: 50744 [Gclust Server

    Lifescience Database Archive (English)

    Full Text Available 50744 Mtu_Rv3568c=bphC Cluster Sequences Related Sequences(4) 300 PROBABLE BIPHENYL...-2,3-DIOL 1,2-DIOXYGENASE BPHC (23OHBP OXYGENASE) (2,3-DIHYDROXYBIPHENYL DIOXYGENASE) (2,3-DIHYDROXYBIPHENYL... 1,2-DIOXYGENASE) (DHBD) 2 1.00e-31 0.0 0.0 0.0 0.0 6.45 0.0 Show 50744 Cluster ID 50744 Sequence ID Mtu_Rv3568c=bph...elated Sequences(4) Sequence length 300 Representative annotation PROBABLE BIPHENYL-2,3-DIOL 1,2-DIOXYGENASE BPH

  17. PROSTATIC INTRAEPITHELIAL NEOPLASIA: HISTOLOGICAL ASSOCIATIONS

    Directory of Open Access Journals (Sweden)

    E. N. Gorbunova

    2009-01-01

    Full Text Available The authors determined the detection rates of prostatic intraepithelial neoplasia (PIN in 2317 patients with benign prostatic hyperplasia (BPH and prostate cancer (PC; and those of chronic prostatitis and fibrosis in patients with PIN, BPH, or PC. There was no difference in median age between the groups. PC was found to be more concurrent with PIN 2 than with BPH. The severer inflammation or fibrosis is, more likely there is a concomitance with PIN 2 or PC. There is evidence for the theory of inflammation is a factor of carcinogenesis. Prostatic fibrosis may also initiate carcinogenesis.

  18. PROSTATIC INTRAEPITHELIAL NEOPLASIA: HISTOLOGICAL ASSOCIATIONS

    Directory of Open Access Journals (Sweden)

    E. N. Gorbunova

    2014-08-01

    Full Text Available The authors determined the detection rates of prostatic intraepithelial neoplasia (PIN in 2317 patients with benign prostatic hyperplasia (BPH and prostate cancer (PC; and those of chronic prostatitis and fibrosis in patients with PIN, BPH, or PC. There was no difference in median age between the groups. PC was found to be more concurrent with PIN 2 than with BPH. The severer inflammation or fibrosis is, more likely there is a concomitance with PIN 2 or PC. There is evidence for the theory of inflammation is a factor of carcinogenesis. Prostatic fibrosis may also initiate carcinogenesis.

  19. Convective Water Vapor Energy for Lower Urinary Tract Symptoms/Benign Prostatic Hyperplasia.

    Science.gov (United States)

    DeLay, Kenneth Jackson; McVary, Kevin T

    2016-08-01

    Benign prostatic hyperplasia (BPH) refers to proliferation of smooth muscle and epithelial cells within the transition zone of the prostate. Half of men over 40 develop histologic BPH. About half of men with BPH develop an enlarged prostate gland, called benign prostatic enlargement; among these, about half develop some degree of bladder outlet obstruction. Bladder outlet obstruction and changes in smooth muscle tone and resistance may result in lower urinary tract symptoms, including storage disturbances (such as daytime urinary urgency, frequency, and nocturia) and voiding disturbances (such as urinary hesitancy, weak urinary stream, straining to void, and prolonged voiding).

  20. The Explore of Prostatic Intraepithelial Neoplasia with MSCT Perfusion Imaging%前列腺上皮内瘤CT灌注成像表现初探

    Institute of Scientific and Technical Information of China (English)

    吕绍茂; 吴莉; 韩丹

    2009-01-01

    目的 了解前列腺增生(BPH)中前列腺上皮内瘤(PIN)的CT灌注成像表现,并初步探讨PIN与前列腺癌(PCa)及良性BPH的灌注成像表现的关系.资料与方法 BPH25例,其中PIN样变9例,良性BPH 16例.PCa 34例,行前列腺病变中心层面灌注扫描,得到各组灌注图(PF)、强化峰值图(PEI)、达峰时间图(TTP)、血容量(BV)值和时间密度曲线(TDC),并对各组灌注值进行对比分析.结果 PIN的PF值大于良性BPH,TTP值小于良性BPH,差异有统计学意义.PIN的TTP值大于PCa组,PF值小于PCa组,差异有统计学意义. 结论 PIN的CT灌注成像表现提示,PIN的PF值和邢值与PCa和良性BPH不同,介于两者间.这种灌注特征可能为早期发现PCa提供一定帮助.%Objective To understand the CT perfusion imaging performance of prostatic intraepithelial neoplasia (PIN) in benign prostatic hyperplasia (BPH) and to explore the relationship between PIN,PC and BPH in the perfusion imaging performance. Materials and Methods All cases underwent routine CT pain scan and perfusion CT scan. The patients were divided into BPH group (25 eases,9 cases of PIN were included) ,and PC group (34cases). All the original maps of perfusion CT scan were transferred to an outline workstation to create color perfusion maps and to measure the blood flow parameters containing PF,PEI,TTP, BV of the prostate cortex and medulla using the function CT software. Results The PF value of PIN was greater than that of benign BPH. The TTP value of PIN was less than that of benign BPH. The differences between PIN and BPH in the TIP value and the PF value had statistical significance. The TrP value of PIN is greater than that of PC group and the PF value is less than that of PC group, which had statistical significant differences. Conclusion The performance of CT perfusion imaging in PIN is different from that of benign BPH and PC. The features of the CT perfusion imaging may be useful for the early detection of PC.

  1. [Combined therapy of voiding irritative disorders after surgical treatment of benign prostatic hyperplasia with Andro-Gin].

    Science.gov (United States)

    Loran, O B; Luk'ianov, I V; Markov, A V

    2005-01-01

    The results of operative and rehabilitative treatment of benign prostatic hyperplasia (BPH) was analysed for 69 BPH patients with postoperative irritative disorders of voiding. The patients were divided into four groups by rehabilitative therapy: group 1 received antibacterial therapy; group 2--antibacterial therapy+alpha-adrenoblockers; group 3--antibacterial therapy+transrectal laser and magnetic physiotherapy; group 4--antibacterial therapy+alpha-adrenoblockers+transrectal laser and magnetic physiotherapy. The worst result was obtained in group 1, the best one in groups 3 and 4 (the response was compatible). Thus, the addition of physiotherapy to the complex of postoperative rehabilitation of patients operated for BPH is justified and provides treatment improvement.

  2. The link between benign prostatic hyperplasia and prostate cancer

    DEFF Research Database (Denmark)

    Ørsted, David Dynnes; Bojesen, Stig E

    2013-01-01

    studies have shown that men with BPH have an increased risk of prostate cancer and prostate-cancer-related mortality, it remains unclear whether this association reflects a causal link, shared risk factors or pathophysiological mechanisms, or detection bias upon statistical analysis. Establishing BPH...... as a causal factor for prostate cancer development could improve the accuracy of prognostication and expedite intervention, potentially reducing the number of men who die from prostate cancer....... therapy. Furthermore, risk factors such as prostate inflammation and metabolic disruption have key roles in the development of both diseases. Despite these commonalities, BPH and prostate cancer exhibit important differences in terms of histology and localization. Although large-scale epidemiological...

  3. Effects of nitrogen on the tolerance of brown planthopper,Nilaparvata Lugens, to adverse environmental factors

    Institute of Scientific and Technical Information of China (English)

    ZHONG-XIANLU; KONG-LUENHEONG; XIAO-PINGYU; CUIHU

    2005-01-01

    The effect of nitrogen content in rice plants on the tolerance of brown planthopper (BPH), Nilaparvata lugens Stal to high temperature, starvation and insecticide, was studied in the laboratory at International Rice Research Institute (IRRI), Philippines. Survival of nymphs and adults, fecundity and egg hatchability were significantly increased by the increase of nitrogen content in host plants at 38℃. Moreover, the survival of nymphs,fecundity and egg hatchability were significantly higher in BPH populations on rice plants with a high nitrogen regimen than those on rice plants with a low nitrogen regimen.Meanwhile, the tolerance of female adults to starvation and nymphs to growth regulator buprofezin on rice plants with a high nitrogen regimen were slightly increased. This indicates that the tolerances of BPH to adverse environmental stresses were positively increased by the application of nitrogenous fertilizer. The outbreak potential of BPH induced by the excessive application of fertilizer in rice fields was also discussed.

  4. Reasons for the weak correlation between prostate volume and urethral resistance parameters in patients with prostatism

    NARCIS (Netherlands)

    R. Kranse (Ries); R. van Mastrigt (Ron); F.H. Schröder (Fritz); J.L.H.R. Bosch (Ruud)

    1995-01-01

    textabstractIn an attempt to increase our understanding of the clinical syndrome of benign prostatic hyperplasia (BPH) an analysis was made of the association between prostate volume as measured by transrectal ultrasound and several reported urodynamically determined urethral resis

  5. Genetic Analysis and Preliminary Mapping of Two Recessive Resistance Genes to Brown Planthopper, Nilaparvata lugens St(a)l in Rice

    Institute of Scientific and Technical Information of China (English)

    Hou Li-yuan; PENG Suo-tang; WEI Xing-hua; Yu Ping; XU Qun; YUAN Xiao-ping; Yu Han-yong; WANG Yi-ping; WANG Cai-hong; WAN Guo; TANG Sheng-xiang

    2011-01-01

    An F2 population derived from the cross of WB01,an introgression line resistant to brown planthopper (BPH) originated from Oryza rufipogon Griff.and a susceptible indica variety 9311,was developed for genetic analysis and gene mapping.The population with 303 Fz:3 families was genotyped by 141 simple sequence repeat (SSR) markers and used for gene mapping.Two softwares,Mapmaker/Exp 3.0 and Windows QTL Cartographer V2.0 were applied to detect QTLs.Totally,two QTLs resistant to BPH,named temporarily as bph22(t) and bph23(t),were identified to locate on chromosomes 4 and 8,individually had LOD values of 2.92and 3.15,and explained 11.3% and 14.9% of the phenotypic variation,respectively.

  6. Medline Plus

    Full Text Available ... is encased within the prostate gland. As a man ages, the prostate typically enlarges in size in ... urinate, and incontinence. Less than half of all men with BPH have symptoms of the disease, or ...

  7. Prostate Ultrasound

    Medline Plus

    Full Text Available ... as detailed as with the transrectal probe. An MRI of the pelvis may be obtained as an ... Benign Prostatic Hyperplasia (BPH) Prostate Cancer Ultrasound- and MRI-Guided Prostate Biopsy Images related to Ultrasound - Prostate ...

  8. Mechanism of Intramolecular Rhodium- and Palladium-Catalyzed Alkene Alkoxyfunctionalizations

    KAUST Repository

    Vummaleti, Sai V. C.

    2015-11-13

    Density functional theory calculations have been used to investigate the reaction mechanism for the [Rh]-catalyzed intramolecular alkoxyacylation ([Rh] = [RhI(dppp)+] (dppp, 1,3-bis(diphenylphosphino)propane) and [Pd]/BPh3 dual catalytic system assisted intramolecular alkoxycyanation ([Pd] = Pd-Xantphos) using acylated and cyanated 2-allylphenol derivatives as substrates, respectively. Our results substantially confirm the proposed mechanism for both [Rh]- and [Pd]/ BPh3-mediated alkoxyfunctionalizations, offering a detailed geometrical and energetical understanding of all the elementary steps. Furthermore, for the [Rh]-mediated alkoxyacylation, our observations support the hypothesis that the quinoline group of the substrate is crucial to stabilize the acyl metal complex and prevent further decarbonylation. For [Pd]/BPh3-catalyzed alkoxycyanation, our findings clarify how the Lewis acid BPh3 cocatalyst accelerates the only slow step of the reaction, corresponding to the oxidative addition of the cyanate O-CN bond to the Pd center. © 2015 American Chemical Society.

  9. Medline Plus

    Full Text Available ... enlarges in size in a process called benign hypertrophy, which means that the gland got larger without ... in several of the symptoms of benign prostatic hypertrophy, or BPH. Symptoms may include a slowed or ...

  10. Medical Tests for Prostate Problems

    Science.gov (United States)

    ... frequency—urination eight or more times a day urinary urgency—the inability to delay urination urinary incontinence—the ... prostatitis and another with BPH may both experience urinary urgency. Sometimes symptoms for the same prostate problem differ ...

  11. Transurethral resection of the prostate a 3 year experience

    Directory of Open Access Journals (Sweden)

    N O Chukwujama

    2011-01-01

    Conclusion: Transurethral resection of prostate is a safe and effective treatment modality in the surgical management of men with bladder outlet obstruction secondary to BPH and advanced CAP in our environment.

  12. Anoctamin 1 (TMEM16A) is essential for testosterone-induced prostate hyperplasia.

    Science.gov (United States)

    Cha, Joo Young; Wee, Jungwon; Jung, Jooyoung; Jang, Yongwoo; Lee, Byeongjun; Hong, Gyu-Sang; Chang, Beom Chul; Choi, Yoon-La; Shin, Young Kee; Min, Hye-Young; Lee, Ho-Young; Na, Tae-Young; Lee, Mi-Ock; Oh, Uhtaek

    2015-08-04

    Benign prostatic hyperplasia (BPH) is characterized by an enlargement of the prostate, causing lower urinary tract symptoms in elderly men worldwide. However, the molecular mechanism underlying the pathogenesis of BPH is unclear. Anoctamin1 (ANO1) encodes a Ca(2+)-activated chloride channel (CaCC) that mediates various physiological functions. Here, we demonstrate that it is essential for testosterone-induced BPH. ANO1 was highly amplified in dihydrotestosterone (DHT)-treated prostate epithelial cells, whereas the selective knockdown of ANO1 inhibited DHT-induced cell proliferation. Three androgen-response elements were found in the ANO1 promoter region, which is relevant for the DHT-dependent induction of ANO1. Administration of the ANO1 blocker or Ano1 small interfering RNA, inhibited prostate enlargement and reduced histological abnormalities in vivo. We therefore concluded that ANO1 is essential for the development of prostate hyperplasia and is a potential target for the treatment of BPH.

  13. About the Prostate

    Science.gov (United States)

    ... develops from the transition zone that surrounds the urethra, or urinary tube. This is why BPH may cause more difficulty with urination than prostate cancer typically does. Treatment-Related Changes Because the prostate ...

  14. Consideration of Drug Therapy in Benign Prostatic Hypertrophy

    Directory of Open Access Journals (Sweden)

    G. Diamantis

    2015-01-01

    Full Text Available Benign prostatic hypertrophy (BPH has become a major global health problem both in its frequency by which it determines the complications and the problems of diagnosis and treatment it requires. BPH is a heterogeneous disease. The symptoms attributed to BPH may have other coexisting causes and growth factors both androgen-dependent and independent, which promotes prostate enlargement. It is well known that prostate size correlates poorly with the symptoms so that reducing prostate using 5-alphareductase or alphablocants inhibitors may not always be sufficient. A better understanding of the pathophysiology of BPH and its interactions with other drugs will help the development of new substances with a better efficiency. This present work aims to be a modest contribution related to medical treatment in benign prostatic hyperplasia and the role that the generalist practitioner should play in managing of this urinary disease quite common in elderly men.

  15. The Role of Minimally Invasive Surgical Techniques in the Management of Large-gland Benign Prostatic Hypertrophy.

    Science.gov (United States)

    Sivarajan, Ganesh; Borofsky, Michael S; Shah, Ojas; Lingeman, James E; Lepor, Herbert

    2015-01-01

    Lower urinary tract symptoms (LUTS) secondary to benign prostatic hypertrophy (BPH) are among the most common medical issues for aging men. Population-based studies suggest that 13.8% of men in their 40s and more than 40% of men over age 60 have BPH. When LUTS are refractory to medical therapy and bothersome enough to warrant surgical intervention, transurethral resection of the prostate and open simple prostatectomy have been the historical reference-standard procedures for decades. Both procedures are highly effective and offer durable improvements in urinary functional outcomes. However, they also have the potential for considerable perioperative complications and morbidity. In an effort to limit surgical morbidity, a variety of minimally invasive surgical techniques to treat BPH have been introduced. Herein we present a comprehensive, evidence-based review of the efficacy and safety profile of modern minimally invasive treatments for large-gland BPH.

  16. Enlarged prostate - what to ask your doctor

    Science.gov (United States)

    What to ask your doctor about enlarged prostate; Benign prostatic hypertrophy - what to ask your doctor; BPH - what to ... nlm.nih.gov/pubmed/23234640 . Roehrborn CG. Benign prostatic hyperplasia: Etiology, pathophysiology, epidemiology, and natural history. In: Wein ...

  17. Effect of Nitrogen on Water Content, Sap Flow, and Tolerance of Rice Plants to Brown Planthopper, Nilaparvata lugens

    Institute of Scientific and Technical Information of China (English)

    LU Zhong-xian; S. VILLAREAL; YU Xiao-ping; K. L. HEONG; HU Cui

    2004-01-01

    Water content (WC) and sap flow from leaf sheath of rice plants with varying nitrogen levels at different growth stages,and fluctuations in relative water content (RWC) of rice plants being damaged by brown planthopper (BPH), Nilaparvata lugens were determined in the laboratory, and the tolerance of rice plants to BPH at different nitrogen regimes was evaluated in the greenhouse at International Rice Research Institute (IRRI), the Philippines. The results indicated that both WC and RWC were increased significantly, as the amount of sap flow from rice plants was reduced statistically, with the increase of nitrogen content in rice plants. RWC in rice plants applied with high nitrogen fertilizer decreased drastically by the injury of BPH nymphs, while the reduced survival duration of rice plants with the increase of nitrogen content was recorded. These may be considered to be one of the important factors in increasing the susceptibility to BPH damage on rice plants applied with nitrogen fertilizer.

  18. Effect of Nitrogen on Water Content, Sap Flow, and Tolerance of Rice Plants to Brown Planthopper, Nilaparvata lugens

    Institute of Scientific and Technical Information of China (English)

    LuZhong-xian; S.VILLAREAL; YuXiao-ping; K.L.HEONG; HuCui

    2004-01-01

    Water content (WC) and sap flow from leaf sheath of rice plants with varying nitrogen levels at different growth stages and fluctuations in relative water content (RWC) of rice plants being damaged by brown planthopper (BPH), Nilaparvata lugens were determincd in the laboratory, and the tolerance of rice plants to BPH at different nitrogen regimes was evaluated in the greenhouse at International Rice Research institute (1RRI), the Philippines. The results indicated that both WC and RWC were increased significantly, as the amount of sap flow from rice plants was reduced statistically, with the in crease of nitrogen content inrice plants. RWC in rice plants applied with high nitrogen fertilizer decreased drastically by the injury, of BPH nymphs, while the reduced survival duration of rice plants with the increase of nitrogen content was recorded. These may be considered to be one of the important factors in increasing the susceptibility' to BPH damage on rice plants applied with nitrogen fertilizer

  19. Prostate Ultrasound

    Medline Plus

    Full Text Available ... also known as benign prostatic hyperplasia (BPH) , with measurements acquired as needed for any treatment planning. detect ... Rarely, a small amount of blood may be present in the sperm or urine following the procedure. ...

  20. Prostate Ultrasound

    Medline Plus

    Full Text Available ... also known as benign prostatic hyperplasia (BPH) , with measurements acquired as needed for any treatment planning. detect ... accredited facilities database . This website does not provide cost information. The costs for specific medical imaging tests, ...

  1. Pharmacological investigation of an Ayurvedic formulation on testosterone propionate-induced benign prostatic hyperplasia rats

    Directory of Open Access Journals (Sweden)

    Soni Hardik

    2014-06-01

    Conclusion: The findings of this study concludes that Herbo-mineral formulation has promising effect on testosterone propionate-induced BPH in male wistar rats. [J Exp Integr Med 2014; 4(2.000: 131-136

  2. Research Progress of Brown Planthopper Resistance Genes in Rice%水稻抗褐飞虱基因研究进展

    Institute of Scientific and Technical Information of China (English)

    祝莉莉; 胡亮; 杜波

    2011-01-01

    综述了水稻抗褐飞虱基因的研究进展.褐飞虱是对水稻为害最严重的害虫之一.它栖息于稻丛基部,吸食韧皮部汁液.褐飞虱具有不同的生物型.在抗性品种的选择压力下,将产生一种新的生物型褐飞虱群体克服该抗性品种.因此,寻找新的抗性基因是培育新的抗褐飞虱水稻品种的关键.合适的水稻抗褐飞虱的鉴定方法是克隆水稻抗褐飞虱基因的基础.常用的方法有苗期集团鉴定、蜜露量测定、电子取食监测系统等.迄今为止,科学家已经在栽培稻和野生稻中定位了21个水稻抗褐飞虱基因,并且Bph14基因已经被武汉大学生命科学学院杂交水稻国家重点实验室成功克隆.该结果为克隆其他水稻抗褐飞虱基因以及研究水稻抗褐飞虱的分子机制奠定了基础.%The research progresses on brown planthopper (Nilap arv ata lugens Stal, BPH) resistance genes in rice were summarized. BPH is one of the most destructive pests in crop production worldwide. They gather at the base part of rice plant and suck assimilates from the phloem. BPH has various biotypos. Under the selection pressure of resistant variety, a new biotype of BPH might come out to overcome the resistance of the variety. Exploring new resistance gene is critical for developing resistant rice variety. Appropriate evaluation of germplasm for BPH resistance is the key to identify resistance genes correctly.There are several types of screening methods such as the standard seodbex screening technique, honey dew test, electronic monitoring system and so on. To date, 21 BPH-resistance genes have been identified from cultivated and wild rice species.Bph14 is the fast report of BPH-resistance gene in rice. These results provide basis for the clone of more BPH resistance genes and the study on molecular mechanism of rice resistance to BPH.

  3. Cost effectiveness comparison of dutasteride and finasteride in patients with benign prostatic hyperplasia - the Markov model based on data from Montenegro

    Directory of Open Access Journals (Sweden)

    Dabanović Vera

    2016-01-01

    Full Text Available Background/Aim. Benign prostatic hyperplasia (BPH is one of the most common disease among males aging 50 years and more. The rise of the prevalence of BPH is related to aging, and since duration of life time period has the tendency of rising the prevalence of BPH will rise as costs of BPH treatment will and its influence on health economic budget. Dutasteride is a new drug similar to finasteride, inhibits enzyme testosterone 5-alpha reductase, diminish symptoms of BPH, reduce risk of the complications and increases quality of life in patients with BPH. But, the use of dutasteride is limited by its high costs. The aim of this study was to compare cost effectiveness of dutasteride and finasteride from the perspective of a purchaser of health care service (Republic Institute for Health Insuranse, Montenegro. Меthods. We constructed a Markov model to compare cost effectivenss of dutasteride and finasteride using data from the available pharmacoeconomic literature and data about socioeconomic sphere actual in Montenegro. A time horizon was estimated to be 20 years, with the duration of 1 year per one cycle. The discount rate was 3%. We performed Monte Carlo simulation for virtual cohort of 1,000 patients with BPH. Results. The total costs for one year treatment of BPH with dutasteride were estimated to be 6,458.00 € which was higher comparing with finasteride which were 6,088.56 €. The gain in quality adjusted life years (QALY were higher with dutasteride (11.97 QALY than with finasteride (11.19 QALY. The results of our study indicate that treating BPH with dutasteride comparing to finasteride is a cost effective option since the value of incremental cost-effectiveness ratio (ICER is 1,245.68 €/QALY which is below estimated threshold (1,350.00 € per one gained year of life. Conclusion. Dutasteride is a cost effective option for treating BPH comparing to finasteride. The results of this study provide new information for health care decision

  4. Exendin-4 shows no effects on the prostatic index in high-fat-diet-fed rat with benign prostatic hyperplasia by improving insulin resistance.

    Science.gov (United States)

    Zheng, J-X; Xiao, Y-C; Hu, Y-R; Hao, M; Kuang, H-Y

    2015-03-01

    Benign prostatic hyperplasia (BPH) is a prevalent disease globally, and accumulating evidence has indicated an association between BPH, insulin resistance (IR) and diabetes. Exendin-4 is widely used in clinics, which could enhance the proliferation of pancreatic β cells. The ability of exendin-4 to promote tumorigenesis has been of concern, and whether exendin-4 would enhance the propagation of BPH is not fully understood. We aimed to determine whether glucagon-like peptide-1 receptors (GLP-1Rs) were expressed in rat prostate and to determine the effect of exendin-4 on prostate of BPH. Male Wistar rats were used and assigned to six groups: normal diet (ND), high-fat diet (HFD), HFD + exendin-4, HFD + BPH, HFD + BPH + exendin-4 and HFD + BPH + rosiglitazone group. After castration, steroids were injected subcutaneously for 4 weeks to induce BPH. Rats were kept on high-fat diet to induce IR. Treatment groups were treated with exendin-4 and rosiglitazone. Prostatic index and HOMA-IR index were used to evaluate the prostatic hyperplasia status and the degree of IR respectively. The expression of GLP-1R was indicated not only by immunohistochemistry, but also by Western blot analysis. The expression of GLP-1R was significantly higher, and HOMA-IR index and body weight significantly decreased after administration of exendin-4. However, no significant differences in the prostatic index were observed between exendin-4 treatment groups and non-exendin-4 treatment groups. Prostatic index was not influenced by exendin-4 maybe by improving IR and weight loss.

  5. Benign Prostatic Hyperplasia

    OpenAIRE

    Gil Ortega, Joan

    2015-01-01

    Benign prostatic hyperplasia (BPH) is a prevalent disease but its molecular mechanism remains unknown. Using human tissue samples from 16 patients diagnosed with BPH, we performed an ultrastructural study to clarify the mechanism and the role of glandular cells in this pathology. We have made a description of all the changes that suffers the prostatic epithelium. We have shown that the glandular architecture presents many non-physiological forms such as papillae and papillary fronds. Basal c...

  6. Stromal Androgen Receptor Roles in the Development of Normal Prostate, Benign Prostate Hyperplasia, and Prostate Cancer

    OpenAIRE

    Wen, Simeng; Chang, Hong-Chiang; Tian, Jing; Shang, Zhiqun; Niu, Yuanjie; Chang, Chawnshang

    2015-01-01

    The prostate is an androgen-sensitive organ that needs proper androgen/androgen receptor (AR) signals for normal development. The progression of prostate diseases, including benign prostate hyperplasia (BPH) and prostate cancer (PCa), also needs proper androgen/AR signals. Tissue recombination studies report that stromal, but not epithelial, AR plays more critical roles via the mesenchymal-epithelial interactions to influence the early process of prostate development. However, in BPH and PCa,...

  7. N-Cadherin in Prostate Cancer: Downstream Pathways and their Translational Application for Castrate-Resistant Prostate Cancer

    Science.gov (United States)

    2012-09-01

    Prostate Center, University of British Columbia, Vancouver, British Columbia, Canada. 5Department of Pathology , Geffen School of Medicine, University...Medicine: doi:10.1038/nm.2236 a 0 .000 -0.100 BPH 0 .011 Untreated ( all grades) NHT treated ( all the time points) Recurrent ( documented...antibody (Clone32, BD Transduction Laboratories). (a) BPH : benign prostate hyperplasia; NHT: neoadjuvant hormone therapy; CRPC: castrate resistant

  8. Expression of tNASP in Prostate Cancer: Opportunities for a Novel Diagnostic and Prognostic Biomarker Development

    Science.gov (United States)

    2013-12-01

    and to distinguish prostate cancer from BPH . Detection of tNASP protein expression levels in needle biopsies and postoperative prostate cancer...samples will be correlated with pathologic indicators of aggressive prostate cancer for diagnostic and prognostic purposes. We propose two specific aims...hypothesis that tNASP-specific antibodies are present in the blood of prostate cancer patients, but not BPH patients or otherwise healthy men. We will test

  9. Interleukin-1α Is a Paracrine Inducer of FGF7, a Key Epithelial Growth Factor in Benign Prostatic Hyperplasia

    OpenAIRE

    Giri, Dipak; Ittmann, Michael

    2000-01-01

    Benign prostatic hyperplasia (BPH) is an extremely common disease of older men in which there is benign overgrowth of the prostatic transition zone, leading to obstruction of urine outflow. FGF7, a potent growth factor for prostatic epithelial cells, is increased by threefold in BPH and is correlated with increased epithelial proliferation in this condition. Immunohistochemistry of normal and hyperplastic prostate revealed that FGF7-expressing fibroblastic cells were present in higher numbers...

  10. Overcoming Drug Resistant Prostate Cancer with APE1/Ref 1 Blockade

    Science.gov (United States)

    2015-10-01

    immunofluorescent images of non- inflamed or non-inflamed human prostates representing non-diseased controls, BPH specimens, or prostate cancer... BPH , and cancer) that exhibited 30 leukocytes per 20x section. Three 20x fields per prostate section were averaged for each data point, and all...metastases We have acquired the first 12 specimens of human prostate cancer metastases from our human pathology core, as described in the proposal

  11. Proteomics of Peripheral Leukocytes in Patients with Elevated Serum Levels of PSA

    Science.gov (United States)

    2012-09-01

    chronic prostatitis (CP) or benign prostatic hyperplasia ( BPH ) [1]. If, after an elevated PSA test, our test could distinguish among these...prostate disease, even though each individual has an elevated PSA level. Two patients with pathology reports showing only enlargement or inflammation but...short amount of time available, we chose to not pursue this work further. BPH / CP PrCA S ig n e d S u m S co re Figure 1. Discrimination between

  12. Overcoming Drug Resistant Prostate Cancer with APE1/Ref-1 Blockade

    Science.gov (United States)

    2015-10-01

    immunofluorescent images of non- inflamed or non-inflamed human prostates representing non-diseased controls, BPH specimens, or prostate cancer... BPH , and cancer) that exhibited 30 leukocytes per 20x section. Three 20x fields per prostate section were averaged for each data point, and all...metastases We have acquired the first 12 specimens of human prostate cancer metastases from our human pathology core, as described in the proposal

  13. Apoptosis and hormonal milieu in ductal system of normal prostate and benign prostatic hyperplasia

    Institute of Scientific and Technical Information of China (English)

    Shu-Jie XIA; Chun-Xiao XU; Xiao-Da TANG; Wan-Zhong WANG; De-Li DU

    2001-01-01

    Aim: To study theapoptotic rate (AR) and the androgen and estrogen milieu in the proximal and distal ductal sys tems of prostate, in order to help exploring the effects of these factors on prostatic growth and the pathogenesis of be nign prostatic hypertrophy (BPH). Methods: The proximal and distal ends of the ductal system were incised from 20 normal prostate as well as the hypertrophic prostate tissue from 20 patients with BPH. The AR was determined by the DNA end-labeling method and dihydrotestosterone (DHT) and estrodiol (E2), by radioimmunoassay. Results:There was no significant difference in DHT and E2 density between the proximal and distal ends of the ductal systems in normal prostate. E2 appeared to be higher in BPH than in normal prostatic tissues, but the difference was statistically in significant. In normal prostatic tissue, the AR was significantly higher in the distal than in the proximal ends of the ductal system ( P < 0.05), while the AR of the proximal ends was significantly higher ( P < 0.01) than that in the BPH tissue. No significant correlation was noted between the DHT and E2 density and the AR both in the normal prostate and BPH tissues. Conclusion: The paper is the first time describing a difference in AR in different regions of the ductal system of normal prostate, while the hormonal milieu is similar, indicating a functional inhomogeneity of these regions. A low AR in the proximal duct, where BPH originates, and an even lower AR in the BPH tissue, sug gesting the participation of apoptosis in the BPH pathogenesis.

  14. [Prostatilen effects on voiding disorders in patients with benign prostatic hyperplasia].

    Science.gov (United States)

    Al'-Shukri, S Kh; Gorbachev, A G; Borovets, S Iu; Belousov, V Ia; Kuz'min, I V

    2005-01-01

    The authors review the experience with prostatilen treatment gained in the urological clinic of the St-Petersburg State Medical University and analyze treatment outcomes for 96 patients with benign prostatic hyperplasia (BPH) aged from 51 to 89 years. Prostatilen proved its efficacy in relieving manifestations of infravesical obstruction in BPH, therefore, prostatilen administration in the treatment of patients with initial or moderate subjective and objective symptoms is justified. Dimexide suppositoria containing 30 mg of prostatilen are preferable.

  15. Low-valent pentafulvene titanium dinitrogen complex as a precursor for cationic titanium complexes

    NARCIS (Netherlands)

    Scherer, Axel; Haase, Detlev; Saak, Wolfgang; Beckhaus, Ruediger; Meetsma, Auke; Bouwkamp, Marco W.; Beckhaus, Rüdiger

    2009-01-01

    Treatment of titanium dinitrogen complex [Cp*(eta(6)-C(5)H(4)=C(10)H(14))Ti](2)(mu-N(2)) (1) with ferrocenium borate, [Cp(2)Fe][BPh(4)], in THF results in oxidation of the titanium center, affording the titanium(IV) pentafulvene compound [Cp*(eta(6)-C(5)H(4)=C(10)H(14))Ti(THF)][BPh(4)] (2). Treatmen

  16. Adoptive Immunotherapy Combined with Hematopoietic Cell Transplantation as a Therapeutic Treatment of Prostate Cancer

    Science.gov (United States)

    2009-07-01

    matched normal donor, has developed into a highly effective therapy for the treatment of hematological malignancies. The canine model has played a...palpation. We employed both of these techniques, performed with the aid of a veterinarian , to determine whether BPH was evident after a DLI of...a veterinarian on day 21 and 47 by rectal palpation. Together, these data suggest there was no indication of BPH based on prostate enlargement

  17. Measuring disease-specific health status in men with benign prostatic hyperplasia. Measurement Committee of The American Urological Association.

    Science.gov (United States)

    Barry, M J; Fowler, F J; O'Leary, M P; Bruskewitz, R C; Holtgrewe, H L; Mebust, W K

    1995-04-01

    In preparation for an outcomes study of benign prostatic hyperplasia (BPH), two measures of disease-specific health status were developed to supplement a symptom score and overall health status measures. The symptom problem index (SPI) captures how troublesome patients find their urinary symptoms. The BPH impact index (BII) measures how much their urinary problems affect various domains of health. A prospective revalidation of the refined instruments (N = 108 BPH patients and 50 controls) documented that both indices had good internal consistency (Cronbach's alpha = 0.88 and 0.79, respectively) and test-retest (r = 0.88 for both) reliabilities, correlated strongly with symptom scores (r = 0.86 and 0.77), and discriminated between BPH and control subjects (receiver-operating characteristic areas = 0.87 and 0.85, respectively). These indices were nearly as responsive as symptom scores in 50 men actively treated for BPH, and much more responsive than a non-disease-specific General Health Index (GHI), a Mental Health Index (MHI), and an Activity Index (AI). Finally, these measures capture most of the health status significance of BPH symptoms. In linear regression models constructed to predict scores on the GHI, MHI, and AI, symptom scores added little explanatory power to the SPI and, particularly, to the BII. These measures help clarify how BPH affects overall health status and function. Such measures have an important role to play in studies of the outcomes of treatment for BPH, and probably for other conditions that interfere with health status and function.

  18. Role of MicroRNA in Aggressive Prostate Cancer

    Science.gov (United States)

    2013-07-01

    prostatic hyperplasia (BPH) specimens (Fig. 8A and B) because BPH is considered benign tissue detected with DAB2IP expression. Furthermore, the...AD_________________ Award Number: W81XWH-11-1-0491 TITLE: Role of microRNA in aggressive prostate ...SUBTITLE Role of microRNA in aggressive prostate cancer 5a. CONTRACT NUMBER W81XWH-11-1-0491 5b. GRANT NUMBER 5c. PROGRAM ELEMENT NUMBER

  19. Impact of catheter on uroflow rate in pressure-flow study

    Institute of Scientific and Technical Information of China (English)

    张鹏; 武治津; 高居忠

    2004-01-01

    @@ The importance of a pressure-flow study in the diagnostic work-up of patients suffering from benign prostatic hyperplasia (BPH) has been recognized. However, there is still uncertainty regarding the role the catheter might play in affecting uroflow rate during a pressure-flow study. In this present study, we retrospectively analyzed voiding data from pressure-flow studies taken before and after catheterization in 44 patients suffering from BPH to investigate whether catheterization has an effect on uroflow rate.

  20. Wheat bran and the induction of intestinal benzo(a)pyrene hydroxylase by dietary benzo(a)pyrene.

    Science.gov (United States)

    Clinton, S K; Visek, W J

    1989-03-01

    The mucosa of the intestine responds to polycyclic aromatic hydrocarbons (PAH) with the rapid induction of benzo(a)pyrene hydroxylase (BPH). Studies were conducted to determine if dietary fiber would reduce exposure of the intestine to dietary benzo(a)pyrene (BP) as indicated by intestinal BPH activity. In all studies, female Sprague-Dawley rats were fed a fiber-free purified diet for 7 d, whereupon they were switched to experimental diets for 48 h. After 48 h their small intestinal mucosa was assayed for BPH activity. Diets for the initial study contained 0, 100, 400, 800, or 1200 mg BP/kg diet, each with and without 10% soft white wheat bran. Enzyme induction with 100 and 400 mg BP/kg diet was partially inhibited by bran, but with higher concentrations of BP there was no protective effect. The inhibition in BP-induced intestinal BPH activity was observed with 10% wheat bran but not with 3.3 or 6.6%. Subsequent studies showed no significant inhibition in BPH induction with cellulose or lignin, whereas all forms of wheat bran (hard red, soft white, or finely ground soft white) caused significant inhibition. In the final study, a diet containing charcoal-broiled beef, a known source of PAH, was compared with diets containing raw beef or soybean protein, each with and without 10% soft white wheat bran. BPH activity remained low with raw beef and soybean protein whether or not fiber was added. However, intestinal BPH activity was raised ninefold by charcoal-broiled beef. The addition of bran reduced BPH activity to 65% of that observed with the fiber-free, charcoal-broiled beef diet.(ABSTRACT TRUNCATED AT 250 WORDS)

  1. Correlation between molecular biomarkers and risk factors for the clinical progression of benign prostatic hyperplasia using tissue microarray immunostaining

    Institute of Scientific and Technical Information of China (English)

    Ma Ding; Yang Bing; Zhou Zhe; Pan Dongliang; Zhang Xianghua

    2014-01-01

    Background The pathogenesis of benign prostatic hyperplasia (BPH) has been widely studied,and several biomarkers are known to play roles in its development.This study aimed to investigate the possible role of cysteine-rich protein 61 (CYR61),vascular endothelial growth factor (VEGF),androgen receptor (AR),interleukin-6 (IL-6),cytochrome c,caspase-3,and proliferating cell nuclear antigen (PCNA) in the clinical progression of BPH.Methods Tissue specimens from 96 BPH cases who underwent transurethral resection of the prostate were processed and transferred to tissue microarrays.Patient age,prostate volume,serum prostate-specific antigen (PSA) level,and International Prostate Symptom Score (IPSS) of all BPH cases were collected before surgery.The expression of CYR61,VEGF,AR,IL-6,cytochrome c,caspase-3,and PCNA was examined by immunostaining in the BPH specimens,and any possible correlation between the different biomarkers and risk factors for BPH clinical progression was analyzed.Results The expression of CYR61,VEGF,AR,IL-6,cytochrome c,caspase-3,and PCNA in the BPH cases was 68.8% (66/96),77.1% (74/96),43.8% (42/96),31.3% (30/96),35.4% (34/96),56.3% (54/96),and 29.2% (28/96),respectively.The expression of both CYR61 and VEGF was positively correlated with patient age,prostate volume,and serum PSA level (P <0.05).Furthermore,cytochrome c and caspase-3 expression were inversely related to prostate volume (P <0.05),and AR expression was positively related to serum PSA level (P <0.05).Conclusion CYR61 and VEGF expression might serve as biomarkers for predicting the clinical progression of BPH due to effects on stromal cell proliferation and angiogenesis.

  2. Intraprostatic ozone therapy: A minimally invasive approach in benign prostatic hyperplasia

    OpenAIRE

    Shabbir Hussain; Sharma, Deepti B.; Fanindra S Solanki; Ajay Pathak; Dhananjay Sharma

    2017-01-01

    Introduction: Transurethral resection of prostate (TURP) remains the golden standard therapy since decades. There are various minimally invasive therapies (MITs) for the treatment of benign prostatic hyperplasia (BPH). Still, there is a need for therapy with lesser side effects and better outcome. We had studied the effect of intraprostatic ozone injection (IPOI) as an MIT for patients with BPH who have failed trial without catheter (TWOC). Materials and Methods: Thirty elderly patients wi...

  3. Comparison of telomerase activity in prostate cancer, prostatic intraepithelial neoplasia and benign prostatic hyperplasia

    Directory of Open Access Journals (Sweden)

    Soleiman Mahjoub

    2006-11-01

    Full Text Available BACKGROUND: Telomerase is a reverse transcriptase enzyme that synthesizes telomeric DNA on chromosome ends. The enzyme is important for the immortalization of cancer cells because it maintains the telomeres. METHODS: Telomerase activity (TA was measured by fluorescence-based telomeric repeat amplification protocol (FTRAP assay in prostate carcinoma and benign prostatic hyperplasia (BPH. RESULTS: TA was present in 91.4% of 70 prostate cancers, 68.8% of 16 prostatic intraepithelial neoplasia (PIN, 43.3% of 30 BPH*, 21.4% of 14 atrophy and 20% of 15 normal samples adjacent to tumor. There was not any significant correlation between TA, histopathological tumor stage or gleason score. In contrast to high TA in the BPH* tissue from the cancer-bearing gland, only 6.3% of 32 BPH specimens from patients only diagnosed with BPH were telomerase activity-positive. CONCLUSIONS: These results indicate that TA is present in most prostate cancers. The high rate of TA in tissue adjacent to tumor may be attributed either to early molecular alteration of cancer that was histologically unapparent, or to the presence of occult cancer cells. Our findings suggest that the re-expression of telomerase activity could be one step in the transformation of BPH to PIN. KEY WORDS: Telomerase activity, prostate cancer, prostatic intraepithelial neoplasia, benign prostatic hyperplasia.

  4. Pathological and morphological features of bladder neck sclerosis

    Directory of Open Access Journals (Sweden)

    Klochay V.V.

    2011-12-01

    Full Text Available The research goal is to estimate a condition of microcirculation in a bladder neck in patients with benign prostatic hyperplasia (BPH and its value in pathogenesis of bladder neck sclerosis. Materials and methods: Work basis included the analysis of treatment results of 1171 patients with BPH who carried complex examination, operative treatment and further supervision. The basic group was made up of 182 patients with BPH who developed bladder neck sclerosis in the postoperative period. The control group consisted of 200patients with BPH who did not develop the described pathology. clinico-anamnestic, laboratory methods and instrumental studies were used. Results: Disturbance of microcirculation in a bladder neck was considered to be a pathogenetic factor of sclerosis development as one of seri¬ous complications of operative treatment in case of BPH. The presence of microcirculatory disorders was confirmed by histological analysis. Inflammation in the bladder neck should be considered as an aggravating factor. Conclusion: The presence of microcirculatory disorders in the bladder neck in patients with BPH requires preoperative corrective therapy for prevention of sclerosis

  5. Apoptotic Pathways Linked to Endocrine System as Potential Therapeutic Targets for Benign Prostatic Hyperplasia

    Science.gov (United States)

    Minutoli, Letteria; Rinaldi, Mariagrazia; Marini, Herbert; Irrera, Natasha; Crea, Giovanni; Lorenzini, Cesare; Puzzolo, Domenico; Valenti, Andrea; Pisani, Antonina; Adamo, Elena B.; Altavilla, Domenica; Squadrito, Francesco; Micali, Antonio

    2016-01-01

    Benign prostatic hyperplasia (BPH) is a chronic condition common in older men that can result in bothersome lower urinary tract symptoms. The molecular mechanisms and networks underlying the development and the progression of the disease are still far from being fully understood. BPH results from smooth muscle cell and epithelial cell proliferation, primarily within the transition zone of the prostate. Apoptosis and inflammation play important roles in the control of cell growth and in the maintenance of tissue homeostasis. Disturbances in molecular mechanisms of apoptosis machinery have been linked to BPH. Increased levels of the glycoprotein Dickkopf-related protein 3 in BPH cause an inhibition of the apoptosis machinery through a reduction in B cell lymphoma (Bcl)-2 associated X protein (Bax) expression. Inhibitors of apoptosis proteins influence cell death by direct inhibition of caspases and modulation of the transcription factor nuclear factor-κB. Current pharmacotherapy targets either the static component of BPH, including finasteride and dutasteride, or the dynamic component of BPH, including α-adrenoceptor antagonists such as tamsulosin and alfuzosin. Both these classes of drugs significantly interfere with the apoptosis machinery. Furthermore, phytotherapic supplements and new drugs may also modulate several molecular steps of apoptosis. PMID:27529214

  6. Investigation of granulomatous prostatitis incidence following intravesical BCG therapy

    Science.gov (United States)

    Balasar, Mehmet; Doğan, Metin; Kandemir, Abdulkadir; Taskapu, Hakan Hakki; Cicekci, Faruk; Toy, Hatice; Gurbuz, Recai

    2014-01-01

    In the present manuscript, we studied the incidence of granulomatous prostatitis in the prostatectomy specimen of the patients who underwent transurethral resection of the prostate (TURP) after superficial bladder cancer treatment with intravesical Bacillus Calmette-Guerin (BCG) and were diagnosed with benign prostate hyperplasia (BPH). The clinical data and histopathological specimen records of 472 patients who underwent TUR-P due to BPH diagnosis, obtained over a period of 6 years in the urology department of Private Konya Hospital, Konya, Turkey, were studied retrospectively. The cases were divided into two groups as (Group I) who did not undergo any treatment and as (Group II) who underwent BCG treatment. The frequency and the clinical course of the cases with granulomatous prostatitis were studied histopathologically. There were in total 472 patients who underwent TUR-P. Out of the 459 patients who did not undergo BCG treatment (Group I), the histopathological specimen records of 262 (57%) was BPH, of 197 (43%) BPH + chronic prostatitis. Of the second group, 13 cases underwent intravesical BCG treatment before surgical intervention due to superficial bladder CA diagnosis. In this group 4 of the cases were diagnosed as (30%) BPH, 9 as (70%) chronic prostatitis + BPH. 6 out of the 9 chronic prostatitis cases were chronic prostatitis, 2 caseous granulomatous prostatitis, 1 non-caseous granulomatous prostatitis. Granulomatous prostatitis cases should require no specific therapy. Conclusion: In patients with obstruction complaints following intravesical BCG treatment, granulomatous prostatitis should also be considered and treatment plans should be made accordingly. PMID:25035779

  7. Diabetes, growth hormone-insulin-like growth factor pathways and association to benign prostatic hyperplasia.

    Science.gov (United States)

    Wang, Zongwei; Olumi, Aria F

    2011-01-01

    Diabetes significantly increases the risk of benign prostatic hyperplasia (BPH) and low urinary tract symptoms (LUTS). The major endocrine aberration in connection with the metabolic syndrome is hyperinsulinemia. Insulin is an independent risk factor and a promoter of BPH. Insulin resistance may change the risk of BPH through several biological pathways. Hyperinsulinemia stimulates the liver to produce more insulin-like growth factor (IGF), another mitogen and an anti-apoptotic agent which binds insulin receptor/IGF receptor and stimulates prostate growth. The levels of IGFs and IGF binding proteins (IGFBPs) in prostate tissue and in blood are associated with BPH risk, with the regulation of circulating androgen and growth hormone. Stromal-epithelial interactions play a critical role in the development and growth of the prostate gland and BPH. Previously, we have shown that the expression of c-Jun in the fibroblastic stroma can promote secretion of IGF-I, which stimulates prostate epithelial cell proliferation through activating specific target genes. Here, we will review the epidemiologic, clinical, and molecular findings which have evaluated the relation between diabetes and development of BPH.

  8. Involvement of Fibroblast Growth Factor Receptor Genes in Benign Prostate Hyperplasia in a Korean Population

    Directory of Open Access Journals (Sweden)

    Hae Jeong Park

    2013-01-01

    Full Text Available Fibroblast growth factors (FGFs and their receptors (FGFRs have been implicated in prostate growth and are overexpressed in benign prostatic hyperplasia (BPH. In this study, we investigated whether single nucleotide polymorphisms (SNPs of the FGFR genes (FGFR1 and FGFR2 were associated with BPH and its clinical phenotypes in a population of Korean men. We genotyped four SNPs in the exons of FGFR1 and FGFR2 (rs13317 in FGFR1; rs755793, rs1047100, and rs3135831 in FGFR2 using direct sequencing in 218 BPH patients and 213 control subjects. No SNPs of FGFR1 or FGFR2 genes were associated with BPH. However, analysis according to clinical phenotypes showed that rs1047100 of FGFR2 was associated with prostate volume in BPH in the dominant model (GA/AA versus GG, P = 0.010. In addition, a significant association was observed between rs13317 of FGFR1 and international prostate symptom score (IPSS in the additive (TC versus CC versus TT, P = 0.0022 and dominant models (TC/CC versus TT, P = 0.005. Allele frequency analysis also showed significant association between rs13317 and IPSS (P = 0.005. These results suggested that FGFR genes could be related to progression of BPH.

  9. Molecular Cloning and Characterization of a New Cold-active Extradiol Dioxygenase from a Metagenomic Library Derived from Polychlorinated Biphenyl-contaminated Soil

    Institute of Scientific and Technical Information of China (English)

    REN He-jun; LU Yang; ZHOU Rui; DAI Chun-yan; WANG Yan; ZHANG Lan-ying

    2012-01-01

    To find new extradiol dioxygenases(EDOs,EC 1.13.11.2),a metagenomics library was constructed from polychlorinated biphenyl-contaminated soil and was screened for some dioxygenase with aromatic ring cleavage activity.A novel EDO,designated as BphC_A,was identified and heterologously expressed in Escherichia coli.The deduced amino acid sequence of BphC_A exhibited a homology of less than 60% with other known EDOs.Phylogenetic analysis of BphC_A suggests that the protein is a novel member of the EDO family.The enzyme exhibits higher substrate affinity and catalytic efficiency toward 3-methylcatechol than toward 2,3-dihydroxybiphenyl or catechol,the preferred substrate of other known EDOs.The optimum activity of purified BphC_A occurred at pH=8.5 and 35 ℃,and BphC_A showed more than 40% of its initial activity at 5 ℃.The activity of purified BphC_A was significantly induced by Mn2+ and slightly reduced bv Al3+,Cu2+ and Zn2+.

  10. Cyclooxygenase 2 (rs2745557) Polymorphism and the Susceptibility to Benign Prostate Hyperplasia and Prostate Cancer in Egyptians.

    Science.gov (United States)

    Fawzy, Mohamed S; Elfayoumi, Abdel-Rahman; Mohamed, Randa H; Fatah, Ihab R Abdel; Saadawy, Sara F

    2016-06-01

    Cyclooxygenase-2 (COX-2), an inducible isoform of cyclooxygenase, has been reported to be correlated with tumorigenesis, tumor progression, and metastasis. We aimed to evaluate the association between COX-2 (rs2745557) polymorphism and prostate cancer (PCa), benign prostate hyperplasia (BPH) risk. We also assessed the influence of other risk factors such as obesity, smoking, diabetes in modulating the risk of PCa in Egyptian men. COX-2 (rs2745557) was genotyped in 112 PC patients, 111 BPH and 120 subjects as a control group. COX-2 and PSA levels were measured by ELISA. We found that GG genotype was associated with a 17-fold increased risk for PCa and 20-fold increased the risk for BPH more than AA genotype. Also, G allele carriers of COX-2 were associated with metastatic cancer (OR = 1.3, P < 0.05) and disease aggressiveness (OR = 3.5, P < 0.001). The coexistence of obesity, smoking, or diabetes with GG genotype may lead to increasing the risk of developing BPH (OR = 3.3, 4, and 2.7, respectively) and of developing PCa (OR = 2.9, 4.9, and 3.2, respectively). Our results showed evidence suggesting the involvement of the COX-2 (rs2745557) polymorphism and its protein in PCa or BPH initiation and progression. Also, the coexistence of COX-2 (rs2745557) and obesity, smoking, or diabetes may lead to the development of PCa or BPH.

  11. Benign Prostatic Hyperplasia: A New Metabolic Disease of the Aging Male and Its Correlation with Sexual Dysfunctions

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    Giovanni Corona

    2014-01-01

    Full Text Available Metabolic syndrome (MetS is a well-recognized cluster of cardiovascular (CV risk factors including obesity, hypertension, dyslipidemia, and hyperglycaemia, closely associated with an increased risk of forthcoming cardiovascular disease and type 2 diabetes mellitus. Emerging evidence indicates that benign prostate hyperplasia (BPH and its related lower urinary tract symptoms (LUTS represent other clinical conditions frequently observed in subjects with MetS. Several modifiable factors involved in MetS determinism, such as inadequate diet, lack of physical exercise, and smoking and drinking behaviours are emerging as main contributors to the development of BPH. The pathogenetic mechanisms underlying the connection between MetS and BPH have not been completely clarified. MetS and its components, hypogonadism, and prostate inflammation probably play an important role in inducing BPH/LUTS. Although historically considered as a “normal” consequence of the aging process, BPH/LUTS should now be faced proactively, as a preventable disorder of the elderly. Type of diet and level of physical activity are now considered important factors affecting prostate health in the aging male. However, whether physical exercise, weight loss, and modifications of dietary habit can really alter the natural history of BPH/LUTS remains to be determined. Further research is advisable to better clarify these points.

  12. Association of CYP1A1, CYP1B1 and CYP17 gene polymorphisms and organochlorine pesticides with benign prostatic hyperplasia.

    Science.gov (United States)

    Kumar, Vivek; Banerjee, Basu Dev; Datta, Sudip Kumar; Yadav, Chandra Shekhar; Singh, Satyender; Ahmed, Rafat Sultana; Gupta, Sanjay

    2014-08-01

    It is well established that steroidal hormones (testosterone and estrogen) increase benign prostatic hyperplasia (BPH) risk. Cytochrome P450 (CYP) enzymes especially CYP1A1, CYP1B1 and CYP17 metabolize these hormones. Apart from that, several endocrine disrupting organochlorine pesticides (OCPs) are reported to mimic the activity of these steroidal hormones. Therefore, functional polymorphisms in these genes and exposure to such pesticides may increase BPH risk further. Our study included 100 newly diagnosed BPH subjects and 100 age-matched healthy male controls. CYP1A1, CYP1B1 and CYP17 polymorphisms were studied using PCR-RFLP and allele-specific PCR method. OCP levels in blood were analyzed by gas chromatography (GC). Levels of p,p'-DDE and endosulfan α were found to be significantly higher amongst BPH subjects as compared to controls (p-values=0.001 and 0.03 respectively) and CYP17 polymorphism was observed to be significantly associated with BPH subjects as compared to controls (p-values=0.03), indicating that these factors may be important risk factors for BPH. However, further studies are required before unequivocal conclusion.

  13. Cultivation, identification and quantification of one species of yeast-like symbiotes, Candida, in the rice brown planthopper, Nilaparvata lugens

    Institute of Scientific and Technical Information of China (English)

    Kun Pang; Sheng-Zhang Dong; Yun Hou; Ya-Lin Bian; Ke Yang; Xiao-Ping Yu

    2012-01-01

    The brown planthopper (BPH),Nilaparvata lugens St(a)l,which is one of the most destructive pests of rice,has been confirmed to harbor yeast-like symbiotes (YLS) in the fat body.Several morphologically different YLS have been previously isolated and cultured in vitro from BPH,but direct evidence is lacking to further clarify whether the cultured YLS were from BPH.In this study,one species of YLS was successfully cultured in vitro and simultaneously verified to exist in the fat body of BPH by sequence analysis and nested polymerase chain reaction (PCR).The cultured YLS isolate in vitro was identified as a member of the genus Candida on the basis of 18S rDNA (ribosomal DNA) and 5.8S-ITS (internal transcribed spacer) rDNA sequence and a phylogenetic analysis of ITS sequences from yeast.Therefore,this yeast isolate was named as Candida-like symbiotes.Candida-like symbiotes was found to exist in fat bodies,ovaries and newly laid eggs of the BPH,but not in the heads,thoraxes and mid-guts.In addition,the number of Candida-like symbiotes in 1 x 106 of purified YLS from BPH fat bodies was speculated to be (5.32 ± 0.22) × 104 on the basis of a quantitative PCR analysis.

  14. Action Mechanism of Ginkgo biloba Leaf Extract Intervened by Exercise Therapy in Treatment of Benign Prostate Hyperplasia

    Directory of Open Access Journals (Sweden)

    Chiung-Chi Peng

    2013-01-01

    overexpression of stromal, and epithelial growth factors associated with chronic inflammation, has become an atypical direct cause of mortality of aged male diseases. Ginkgo possesses anti-inflammatory, blood flow-enhancing, and free radical scavenging effects. Considering strenuous exercise can reduce BPH risks, we hypothesize Ginkgo + exercise (Ginkgo + Ex could be beneficial to BPH. To verify this, rat BPH model was induced by s.c. 3.5 mg testosterone (T and 0.1 mg estradiol (E2 per head per day successively for 8 weeks, using mineral oil as placebo. Cerenin® 8.33 μL/100 g was applied s.c. from the 10th to the 13th week, and simultaneously, Ex was applied (30 m/min, 3 times/week. In BPH, Ginkgo alone had no effect on T, 5α-reductase, and dihydrotestosterone (DHT, but suppressed androgen receptor (AR, aromatase, E2 and estrogen receptor (ER, and the proliferating cell nuclear antigen (PCNA; Ex alone significantly reduced T, aromatase, E2, ER, AR, and PCNA, but highly raised DHT. While Ginkgo + Ex androgenically downregulated T, aromatase, E2, and ER, but upregulated DHT, AR, and PCNA, implying Ginkgo + Ex tended to worsen BPH. Conclusively, Ginkgo or Ex alone may be more beneficial than Ginkgo + Ex for treatment of BPH.

  15. Transgenic rice homozygous lines expressing GNA showed enhanced resistance to rice brown planthopper

    Institute of Scientific and Technical Information of China (English)

    2001-01-01

    Mature seed-derived calli from two elite Chinese japonica rice (Oryza sativa L.) cultivars Eyi 105 and Ewan 5 were co-transformed with two plasmids, pWRG1515 and pRSSGNA1, containing the selectable marker hygromycin phosphotransferase gene (hpt), the reporter β-glucuronidase gene (gusA) and the snowdrop (Galanthus nivalis)lectin gene (gna) via particle bombardment. 61 independent transgenic rice plants were regenerated from 329 bombarded calli. 79% transgenic plants contained all the three genes, revealed by PCR/Southern blot analysis. Western blot analysis revealed that 36 out of 48 gna-containing transgenic plants expressed GNA (75 %) at various levels with the highest expression being approximately 0.5% of total soluble protein. Genetic analysis confirmed Mendelian segregation of transgenes in progeny. From the R2 generations whose R1 parent plants showing 3:1 Mendelian segregation patterns,we identified five independent homozygous lines containing and expressing all the three transgenes. Insect bioassay and feeding tests showed that these homozygous lines had significant inhibition to rice brown planthopper (Nilaparvata lugens, BPH) by decreasing BPH survival and overall fecundity, retarding BPH development and declining BPH feeding.These BPH-resistant lines have been incorporated into rice insect resistance breeding program. This is the first report that homozygous transgenic rice lines expressing GNA, developed by genetic transformation and through genetic analysis-based selection, conferred enhanced resistance to BPH, one of the most damaging insect pests in rice.

  16. Characteristic gene expression profiles of benign prostatic hypertrophy and prostate cancer.

    Science.gov (United States)

    Endo, Takumi; Uzawa, Katsuhiro; Suzuki, Hiroyoshi; Tanzawa, Hideki; Ichikawa, Tomohiko

    2009-09-01

    The molecular mechanism playing a role in the development of benign prostate hypertrophy (BPH) and prostate cancer (PC) is not well defined. We performed microarray analysis to assess the gene expression change in BPH and PC, and performed network analysis. Normal prostate, BPH and PC tissues were obtained from patients who underwent an operation at Chiba University Hospital. Using Affymetrix Human Genome U133 Plus2.0 Array, we identified genes differentially expressed. The identified genes were analyzed using the Ingenuity Pathway Analysis (IPA) to investigate the functional network and gene ontology. The microarray analysis identified 402 genes in BPH and 141 genes in PC, which were up- or down-regulated at least 5.0-fold change in PC at all dose points. Analysis using IPA software revealed eight networks in BPH and five networks in PC. We narrowed these down to the top five genes, which were up- or down-regulated on the networks in their characteristic manner. From this new perspective, comparing BPH and PC in microarray studies, our data showing gene expression profiles provide candidate genes for better understanding of disease and new therapeutic targets.

  17. Apoptotic Pathways Linked to Endocrine System as Potential Therapeutic Targets for Benign Prostatic Hyperplasia

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    Letteria Minutoli

    2016-08-01

    Full Text Available Benign prostatic hyperplasia (BPH is a chronic condition common in older men that can result in bothersome lower urinary tract symptoms. The molecular mechanisms and networks underlying the development and the progression of the disease are still far from being fully understood. BPH results from smooth muscle cell and epithelial cell proliferation, primarily within the transition zone of the prostate. Apoptosis and inflammation play important roles in the control of cell growth and in the maintenance of tissue homeostasis. Disturbances in molecular mechanisms of apoptosis machinery have been linked to BPH. Increased levels of the glycoprotein Dickkopf-related protein 3 in BPH cause an inhibition of the apoptosis machinery through a reduction in B cell lymphoma (Bcl-2 associated X protein (Bax expression. Inhibitors of apoptosis proteins influence cell death by direct inhibition of caspases and modulation of the transcription factor nuclear factor-κB. Current pharmacotherapy targets either the static component of BPH, including finasteride and dutasteride, or the dynamic component of BPH, including α-adrenoceptor antagonists such as tamsulosin and alfuzosin. Both these classes of drugs significantly interfere with the apoptosis machinery. Furthermore, phytotherapic supplements and new drugs may also modulate several molecular steps of apoptosis.

  18. Role of Transition Zone Index in the Prediction of Clinical Benign Prostatic Hyperplasia

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    Muhammet Güzelsoy

    2016-12-01

    Full Text Available Objective The objective of this study was to determine the role of the transition zone (TZ index (TZI in the prediction of clinical benign prostatic hyperplasia (BPH in patients who underwent transurethral prostatectomy (TUR-P and to analyze the correlation between the amount of resected tissue and TZ volume (TZV. Materials and Methods Twenty-six male clinical BPH patients with obstructive complaints and 17 male benign prostate enlargement (BPE patients without any complaints were included in the study. Both the groups were over the age of 50. Clinical BPH patients underwent complete TUR-P. Statistical analysis was done with SPSS. Sensitivity, specificity, positive and negative predictive values of TZI-as a method of assessing clinical BPH-were measured. Results There was a statistically significant difference in prostate volume, uroflowmetry patterns, prostate-specific antigen (PSA, International prostate symptom score (IPSS, TZV and TZI between the two groups. There was a correlation between TZV and the amount of resected tissue (r=0.97; p0.40 has a high level of sensitivity and specificity in the prediction of clinical BPH among patients who undergo TUR-P due to obstructive symptoms and reported as BPH. There is a strong correlation between the amount of resected tissue and TZV. TZI is a valuable tool in diagnosis, and TZV gives valuable information about the patient to the surgeon.

  19. miRNA Regulation Network Analysis in Qianliening Capsule Treatment of Benign Prostatic Hyperplasia

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    Liya Liu

    2015-01-01

    Full Text Available Objective. The objective of this study was to evaluate the molecular mechanism by which Qianliening capsule (QC treats benign prostatic hyperplasia (BPH. Methods. Benign prostatic hyperplasia epithelial cell line BPH-1 was treated with 0, 1.25, 2.5, and 5 mg/mL QC for 48 h, respectively. Evaluation of cell viability and observation of morphologic changes of BPH-1 cell gene expression and miRNA expression profiles were analyzed. Real-time quantitative PCR was used to confirm changes in miRNA and gene expression. GO and KEGG pathway-based approaches were used to investigate biological functions and signaling pathways affected by differentially expressed mRNAs. Results. QC inhibited BPH-1 cell proliferation. Differential expression of 19 upregulated and 2 downregulated miRNAs was observed in QC-treated BPH-1 cells compared to untreated control cells. 107 upregulated and 71 downregulated genes were identified between the two groups. Significantly enriched signaling pathways based on deregulated mRNAs were mainly involved in regulation of cell proliferation, apoptosis, and so on. Additionally, miRNA-mRNA network analysis integrated these miRNAs and genes by outlining interactions of miRNA and related genes. Conclusion. The study was the first report of differentially expressed miRNA and mRNA in QC-treated BPH-1 cells.

  20. The relationship of pathological types of benign prostatic hyperplasia to serum TPSA, FPSA and the ratio of FPSA to TPSA%良性前列腺增生病理类型与血清FPSA和TPSA及其比值的关系

    Institute of Scientific and Technical Information of China (English)

    陈日敢; 赖国修; 吕腾荣

    2011-01-01

    目的 观察总前列腺特异抗原(TPSA)、游离前列腺特异抗原(FPSA)、FPSA与TPSA 比值(F/T) 在良性前列腺增生(BPH)病理类型诊断中的作用.方法 检测98例BPH(腺瘤样型39例,纤维肌型35例,纤维肌腺瘤样型24例)、30例健康患者(对照组)的TPSA、FPSA,并计算出相应的F/T值.结果 BPH组FPSA、TPSA均显著高于对照组,F/T低于对照组,差异具有显著性(P0.05).结论 腺瘤样型BPH患者血清FPSA、TPSA高于纤维肌型和纤维肌腺瘤样型,对诊断BPH病理类型有一定程度上诊断价值,从而为临床指导用药提供参考.%Objective To observe the serum total prostate specific antigen ( TPSA ), free prostate specific antigen ( FPSA ), and the ratio of FPSA to TPSA ratio ( F/T ) in the diagnosis of pathological types of benign prostatic hyperplasia ( BPH ).Methods Ninety - eight cases of BPH ( adenomatoid type 39 cases, muscle fiber type 35 cases, fibromuscular adenomatoid type 24 cases ) and 30 healthy subjects ( control group ) were included in this study.Serum TPSA and FPSA were detected and the corresponding F/T value was calculated.Results FPSA and TPSA were significantly higher, F/T was lower in BPH group than the control group ( P < 0.05 ).Comparison of serum levels of FPSA and TPSA in different pathological types of BPH showed that serum FPSA and TPSA were higher in adenomatoid type than those in muscle fiber type group and fibromuscular adenomatoid type group( P < 0.05 ), however, there were no differences in the levels of FPSA and TPSA between muscle fiber group and fibromuscular adenomatoid group( P > 0.05 ).In addition, there was no significant difference in F/T among each type of BPH ( P > 0.05 ).Conclusion The finding that serum FPSA and TPSA levels were higher in patients with adenomatoid type BPH than those in patients with muscle fiber type and fibromuscular adenomatoid type BPH may provide help for the diagnosis of pathological types of BPH and for the treatment in

  1. Expression of αs1-casein in benign prostatic hyperplasias%乳蛋白αs1-Casein在良性前列腺增生患者中的表达

    Institute of Scientific and Technical Information of China (English)

    赵国栋; 王驭良; 张晓鹏; 许克新; 王晓峰

    2009-01-01

    目的 检测良性前列腺增生(BPH)患者组织中蛋白表达的特点,了解有无与BPH相关的特异性蛋白表达.方法 通过双向电泳方法 所发现的检测BPH组织特异性蛋白表达,αs1-Casein进行定量免疫组化研究来验证该蛋白表达的特异性.结果 通过对正常前列腺组织,BPH及前列腺癌组织进行免疫组织化学染色,发现αs1-Casein在90%(20/22)的BPH组织中呈弱到强表达,而在正常前列腺组织中未见表达(0/10),在前列腺癌组织中的表达也不足10%(3/30).结论 αs1-Casein可能是一种BPH诊断的生物标记物.%Objective To analyze the characteristic of protein profile of benign prostatic hyperplasia (BPH) tissue and to further find the special proteins associated with BPH. Methods Protein profiles of human expressed prostatic secretion (EPS) samples obtained from BPH patients and normal males were comparatively analyzed by two-dimensional gel electrophoresis. In the resulting electrophoretograms, several differentially expressed proteins were identified. In this study, a distinct expressed protein αs1-casein was validated in BPH. Results Immunohistochemistry analysis showed that αs1-casein was mainly distributed in the epithelium of hyperplastic prostate. The level of the exression of αs1-casein in the tissue of BPH was found to be higher than that in prostatic carcinoma; whereas no asl-casein was detected in normal tissue.Western blotting analysis was also revealed same expression pattern of asl-casein was detected in BPH-EPS and BPH-free EPS. Conclusion asl-casein might be associated with BPH development and be used as a potential biological marker for the diagnosis of BPH.

  2. Effects of alpha-mangostin from mangosteen pericarp extract and imidacloprid on Nilaparvata lugens (Stal.) and non-target organisms: toxicity and detoxification mechanism.

    Science.gov (United States)

    Bullangpoti, Vasakorn; Visetson, Suraphon; Milne, John; Milne, Manthana; Sudthongkong, Chaiwud; Pronbanlualap, Somchai

    2007-01-01

    The brown planthopper, Nilaparvato lugens Stat. (BPH) is the most devastating insect pest in rice fields. Outbreaks of BPH, which are resistant to many synthetic insecticides, can cause total rice crop loss. This research was done to evaluate the efficiency of extracts of mangosteen pericarp (Garcina mangostana L.) as an alternative control of BPH Thailand strain. Topical spraying was applied to various stages of nymphal and adult BPH to determine toxicity. An ethanol extract of mangosteen pericarp extract gave the best control of BPH, with LC50 of 4.5% w/v (r2 = 0.95) with 3rd instar BPH nymphs when compared with the other solvents, hexane, acetone and dichloromethane. The active compound, alpha-mangostin showed an LC50 of 5.44%w/v (r2 = 0.88). The toxicity of this extract was less than that of Imidacloprid which showed an LC50 of 0.0042% w/v (r2 = 0.99). The toxicity to non-target organisms was determined. This extract showed toxicity to guppies ((LC50 = 2.53 and 4.27 ppm for females and males, respectively; r2 = 0.97 and 0.97, respectively), bees (LC50 = 4.38% w/v, r2 = 0.95) and mice (no oral acute toxicity and no dermal inflammation but showed eye irritation in 1 day which became normal within 3 days). In vitro detoxification enzyme activities of carboxylesterase, acetylcholinesterase and glutathione-s-transferase from BPH after 24 hours exposure were also observed. Carboxylesterase showed stronger activity than other enzymes. Toxicity in terms of LC50 values of both the extract and imidacloprid treatments increased in each generation. The LC50 values for each generation were 4.22-6.67 after sequential spraying. After the ethanol extract was kept at 4 degrees C, room temperature and 55 degrees C for 3 months, the quantity of alpha-mangostin and the BPH control efficiency was lower at 55 degrees C than those for other temperatures. The results from this research indicate that mangosteen pericarp extract can be an alternative insecticide for the control of BPH

  3. 组织学前列腺炎对前列腺增生患者尿动力学参数及尿潴留的影响%The Influence of Histological Prostatitis to Urodynamic Parameters and Urinary Retention in Patients with Benign Prostatic Hyperplasia

    Institute of Scientific and Technical Information of China (English)

    黄宏双; 郑晓娴; 谢锦来; 陈智伟; 晏湘山

    2015-01-01

    Objective To discuss the influence of histological prostatitis (HP)on urodynamic parameters and urinary retention in patients with benign prostatic hyperplasia (BPH ). Methods 98 patients who underwent bipolar transurethral plasmakinetic prostatectomy (TUPKP)due to BPH were retrospectively reviewed and divided into two groups,BPH group and BPH with HP group,based on pathological characteristics of tissue samples. The International prostate symptom score(IPSS),prostate volume(PV),PSA level and urodynamic features including maximum urine flow,maximum bladder capacity, maximum detrusor contractility and bladder outlet obstruction index(BOOI)were compared between the two groups. The occurrence rate of involuntary detrusor contractions and urinary retention were analyzed in both groups. Results Patients in BPH with HP group presented significant higher IPSS total scores (P =0.020),higher PSA level(P =0.009),higher bladder outlet obstruction index (P =0.003)and lower maximum bladder capacity(P =0.004)than the patients in BPH group. Patients in BPH with HP group showed a higher occurrence rate of involuntary detrusor contraction(P =0.041)and urinary retention than that in the other group(P =0.010). Conclusion The occurrence of HP is associated with severer lower urinary tract symptoms,higher PSA level,urodynamic parameters changes such as lower maximum blad-der capacity,severer bladder outlet obstruction,higher proportion of detrusor over activity and urinary re-tention in patients with BPH.%目的:探讨合并组织学前列腺炎(HP)对前列腺增生患者的尿动力学参数及尿潴留的影响。方法回顾性分析98例接受经尿道前列腺等离子双极电切术治疗的良性前列腺增生(BPH)患者资料,根据病理诊断分为单纯 BPH 组和 BPH 合并 HP 组,比较国际前列腺症状评分(IPSS)、前列腺体积(PV)、前列腺特异性抗原(PSA)、最大尿流率、最大膀胱容量(MBC)、最大逼尿肌收缩力和膀胱出口梗阻指

  4. Inhibitory effects of Tripterygium wilfordii multiglycoside on benign prostatic hyperplasia in rats.

    Science.gov (United States)

    Shen, Hai-Nan; Xu, Yuan; Jiang, Zhen-Zhou; Huang, Xin; Zhang, Lu-Yong; Wang, Tao

    2015-06-01

    The present study was designed to evaluate the inhibitory effects of Tripterygium wilfordii multiglycoside (GTW) against testosterone-induced benign prostatic hyperplasia (BPH) in rats. A total of 45 rats were randomly divided into five groups: Group I, vehicle control group (sham-operated and treated with vehicle); Group II, BPH group; Group III, BPH rats treated with finasteride at a dose of 5 mg·kg(-1); and Groups IV and V, BPH rats treated with GTW at dose levels of 10 and 20 mg·kg(-1), respectively. The drugs were administered orally once a day for 14 days. Prostate weight, prostatic index, and the testosterone and dihydrotestosterone (DHT) levels in serum and prostate, and the serum prostate specific antigen (PSA) levels were measured; prostate tissues were taken for histopathological examination; and serum biochemical analysis was also performed. The BPH rats displayed an increase in prostate weight, prostatic index with increased testosterone and DHT levels in both the serum and prostate, and increased serum PSA levels. GTW treatment at both doses resulted in significant reductions in prostate weight, prostatic index, testosterone and DHT levels in both the serum and prostate, and serum PSA levels, compared with BPH group. Histopathological examination also indicated that GTW treatment at both doses inhibited testosterone-induced prostatic hyperplasia. Serum biochemical analysis showed that the liver and renal functions were normal. In conclusion, GTW inhibited testosterone-induced prostatic hyperplasia in rats, without host toxicity, providing a basis for the development of GTW as a novel therapy for BPH.

  5. Lifetime occupational and recreational physical activity and risk of benign prostatic hyperplasia.

    Science.gov (United States)

    Dal Maso, Luigino; Zucchetto, Antonella; Tavani, Alessandra; Montella, Maurizio; Ramazzotti, Valerio; Polesel, Jerry; Bravi, Francesca; Talamini, Renato; La Vecchia, Carlo; Franceschi, Silvia

    2006-05-15

    To investigate the relation between occupational and recreational physical activity (PA) in different periods of life and the risk of benign prostatic hyperplasia (BPH), we conducted a hospital-based, case-control study in Italy. The study included 1,369 histologically confirmed BPH and 1,451 controls, admitted to the same hospitals for acute, nonneoplastic diseases. Odds ratios (ORs) and 95% confidence intervals (CIs) of BPH, according to lifetime PA, were obtained by unconditional multiple logistic regression models, including terms for age, study center and education. Compared to the lowest level of occupational PA, the multivariate ORs for BPH for the heavy/strenuous level were 0.6 (95% CI, 0.4-0.8) at age 15-19, 0.6 (95% CI, 0.4-0.8) at age 30-39 and 0.7 (95% CI, 0.5-0.9) at age 50-59. Moreover, compared to or=5 hr/week) were 0.5 (95% CI, 0.4-0.7) at age 15-19, 0.6 (95% CI, 0.5-0.8) at age 30-39, and 0.7 (95% CI, 0.5-0.8) at age 50-59. All inverse trends in risk were significant, and no heterogeneity was found by reason of BPH-diagnosis, age at diagnosis, and body mass index (BMI). The inverse association between PA and BPH risk may be due to favorable hormonal correlates of PA, but residual confounding by socioeconomic covariates cannot be excluded. A moderate PA at any ages may help reducing a sizeable number of BPH.

  6. Chrysophanic acid reduces testosterone-induced benign prostatic hyperplasia in rats by suppressing 5α-reductase and extracellular signal-regulated kinase.

    Science.gov (United States)

    Youn, Dong-Hyun; Park, Jinbong; Kim, Hye-Lin; Jung, Yunu; Kang, JongWook; Jeong, Mi-Young; Sethi, Gautam; Seok Ahn, Kwang; Um, Jae-Young

    2017-02-07

    Benign prostatic hyperplasia (BPH) is one of the most common chronic diseases in male population, of which incidence increases gradually with age. In this study, we investigated the effect of chrysophanic acid (CA) on BPH. BPH was induced by a 4-week injection of testosterone propionate (TP). Four weeks of further injection with vehicle, TP, TP + CA, TP + finasteride was carried on. In the CA treatment group, the prostate weight was reduced and the TP-induced histological changes were restored as the normal control group. CA treatment suppressed the TP-elevated prostate specific antigen (PSA) expression. In addition, 5α-reductase, a crucial factor in BPH development, was suppressed to the normal level close to the control group by CA treatment. The elevated expressions of androgen receptor (AR), estrogen receptor α and steroid receptor coactivator 1 by TP administration were also inhibited in the CA group when compared to the TP-induced BPH group. Then we evaluated the changes in three major factors of the mitogen-activated protein kinase chain during prostatic hyperplasia; extracellular signal-regulated kinase (ERK), c-Jun-N-terminal kinase (JNK) and p38 mitogen-activated protein kinase (p38). While ERK was elevated in the process of BPH, JNK and p38 was not changed. This up-regulated ERK was also reduced as normal by CA treatment. Further in vitro studies with RWPE-1 cells confirmed TP-induced proliferation and elevated AR, PSA and p-ERK were all reduced by CA treatment. Overall, these results suggest a potential pharmaceutical feature of CA in the treatment of BPH.

  7. 水稻褐飞虱综合治理研究与示范——农业公益性行业专项“水稻褐飞虱综合防控技术研究”进展%Progress in research on the integrated management of the brown planthopper, Nilaparvata lugens (St(a)l) in China

    Institute of Scientific and Technical Information of China (English)

    林拥军; 罗举; 娄永根; 祝增荣; 唐启义; 吕仲贤; 徐红星; 郑许松; 黄凤宽; 彭兆谱; 何光存; 华红霞; 陈荣智; 郭慧芳; 陈其志; 万丙良; 吕亮; 林华峰; 刘芳; 肖汉祥; 侯茂林; 姚英娟; 何予卿; 杨长举; 翟保平; 沈晋良; 张开军; 薛晓峰; 傅强

    2011-01-01

    Because of the magnitude of the losses to rice production caused by the brown planthopper ( BPH, Nilaparvata lugens (Stal) ) , and the resistance of BPH to chemical insecticides, the frequency of BPH outbreaks, breeding of insect resistant rice varieties, insecticide resistance monitoring, development of new pesticides , new ecological management technologies, prediction technologies and the integration and demonstration of sustainable pest management technologies were investigated in central, southern and eastern China and other representative regions.The results show that BPH collected from Vietnam, Laos and southern China were mainly biotype Ⅱ , with the next most common biotype being biotype Ⅲ. Paddy field microclimate was the key factor for BPH escaping high temperature, as morning temperature increased, a large number of BPH moved to within 20 cm of the rice base to thereby minimizing the adverse effects of high temperature. Different methods of transplanting rice seedlings had a great influence on the densities of BPH; BPH densities in handed-planted rice fields were the highest, followed by those in machine-planted rice fields, with densities in direct seeded rice fields the lowest. The abundance of BPH in super hybrid rice was obviously higher than in conventional hybrid rice and high levels of nitrogen promoted BPH occurrence. Furthermore, there was an interactive effect between different rice varieties and nitrogen levels with regard to BPH abundance. BPH in China have high resistance to imidacloprid (168.1-561.5) , low to moderate resistance to buprofezin (4.2-33.1) and are sensitive to slightly resistant to nitenpyram and chlorpyrifos. Four effective pesticides with low toxicity to humans were selected from those sold on the current market; Thiamethoxam, Pymetrozine, Nitenpyram and Fenobucard. We developed three kinds of new compound pesticides, one of which has been officially registered by the Ministry of Agriculture' s Pesticide Quarantine

  8. Sperm quality and selected biochemical parameters of seminal fluid in dogs with benign prostatic hyperplasia.

    Science.gov (United States)

    Krakowski, L; Wąchocka, A; Brodzki, P; Wrona, Z; Piech, T; Wawron, W; Chałabis-Mazurek, A

    2015-09-01

    Benign prostatic hyperplasia (BPH) in dogs is most commonly associated with age and increasing concentrations of dihydrotesterone, a hormone that stimulates growth and secretion of the prostatic epithelial cells. During this process, the biochemical composition of prostatic secretion changes, which can affect the quality of semen and limit the ability of the sperm to contribute to fertilization. Therefore, the present study was conducted to examine possible correlation between BPH and biological quality of semen. The study was performed in 11 sexually mature dogs of various breeds. Animals were divided into two groups: healthy dogs (Group I; n = 5; mean age 4.32; SEM = 1.28) and dogs with BPH (Group II n = 6; mean age 6.16; SEM = 0.65). Semen and prostate secretions were collected and evaluated in this study. Standard semen examinations were conducted in the ejaculates collected; moreover, the extent of apoptosis and DNA defragmentation was determined. The selected biochemical parameters were determined in the prostate secretion. According to the examination results, there were no significant differences in standard semen parameters between the two groups of dogs. Nevertheless, morphological tests of semen in dogs with BPH demonstrated elevated percentages of primary defects in spermatozoa. A significant increase (P = 0.01) in DNA defragmentation of sperm was found in dogs with BPH. Moreover, changes in the biochemical composition of prostate secretion were demonstrated. In dogs with BPH, pH of prostate secretions was greater (P = 0.03), concentrations of cholesterol increased while concentrations of Zn and Cu decreased. The study findings reveal that BPH does not change semen quality in dogs.

  9. Will climate change affect outbreak patterns of planthoppers in Bangladesh?

    Science.gov (United States)

    Ali, M P; Huang, Dingcheng; Nachman, G; Ahmed, Nur; Begum, Mahfuz Ara; Rabbi, M F

    2014-01-01

    Recently, planthoppers outbreaks have intensified across Asia resulting in heavy rice yield losses. The problem has been widely reported as being induced by insecticides while other factors such as global warming that could be potential drivers have been neglected. Here, we speculate that global warming may increase outbreak risk of brown planthopper (Nilaparvata lugens Stål.). We present data that demonstrate the relationship between climate variables (air temperature and precipitation) and the abundance of brown planthopper (BPH) during 1998-2007. Data show that BPH has become significantly more abundant in April over the 10-year period, but our data do not indicate that this is due to a change in climate, as no significant time trends in temperature and precipitation could be demonstrated. The abundance of BPH varied considerably between months within a year which is attributed to seasonal factors, including the availability of suitable host plants. On the other hand, the variation within months is attributed to fluctuations in monthly temperature and precipitation among years. The effects of these weather variables on BPH abundance were analyzed statistically by a general linear model. The statistical model shows that the expected effect of increasing temperatures is ambiguous and interacts with the amount of rainfall. According to the model, months or areas characterized by a climate that is either cold and dry or hot and wet are likely to experience higher levels of BPH due to climate change, whereas other combinations of temperature and rainfall may reduce the abundance of BPH. The analysis indicates that global warming may have contributed to the recent outbreaks of BPH in some rice growing areas of Asia, and that the severity of such outbreaks is likely to increase if climate change exaggerates. Our study highlights the need to consider climate change when designing strategies to manage planthoppers outbreaks.

  10. A Review on Clinical Pharmacokinetics of Tamsulosin in Patients with Benign Prostatic Hyperplasia

    Directory of Open Access Journals (Sweden)

    Tolou Ghamari

    2016-08-01

    Full Text Available Context Benign prostatic hyperplasia (BPH that disturbs aged males is described as the abundant, chronic progressive disorder usually associated with lower urinary tract symptoms. The alpha 1A-adrenergic receptor antagonist, tamsulosin (Flomax®/Omnic®, seems to be an appropriate management from the view point of urological surgeons. The current review aimed to evaluate the clinical pharmacological properties of tamsulosin in prostate disorders. Evidence Acquisition United States national library of medicine (PubMed, NLM were searched from April 1991 to March 2016. The Mesh terms were: tamsulosin, tamsulosin pharmacokinetics in urology, tamsulosin in BPH and clinical pharmacokinetics of tamsulosin in BPH. Published clinical trials and review articles applicable to pharmacotherapy of tamsulosin in BPH were reviewed. Results In patients with mild international prostate symptom score (< 8 to moderate symptoms of BPH, initial treatment with an alpha 1-adrenergic antagonist monotherapy were useful. With a half-life of 9 - 13 hours, the oral bioavailability of drug was reported 100%. The drug is metabolized by liver. Excretion through renal was reported 76%. Initial dose of drug was 0.4 mg/day and the maximum dose was reported 0.8 mg/day. The drug could cause dizziness, sever drowsiness and problems in thinking, driving and many other complications. Other serious adverse reactions could be mentioned as hives, rash, itching, and difficulty in breathing, swelling of face, lips, tongue and throat. Tamsulosin therapy should be stopped in patients who experience hypotension. Conclusions To treat BPH therapeutically, it is necessary to stabilize severities of the patient’s symptoms with potential side effects of the treatment. Tamsulosin blocks alpha 1-receptors in smooth muscles both in the bladder neck and prostate, which leads to relaxation and subsequently less resistance to urinary flow. By advancement of tamsulosin, pharmacotherapy strategies could

  11. DIFFERENTIATION BETWEEN BENIGN PROSTATIC HYPERPLASIA AND PROSTATE CANCER IN THE TRANSITIONAL ZONE EVALUATED BY 1H MAGNETIC RESONANCE SPECTROSCOPIC IMAGING

    Institute of Scientific and Technical Information of China (English)

    Sa-ying Li; Min Chen; Rui Wang; Cheng Zhou

    2007-01-01

    To quantitatively evaluate the metabolic changes of benign prostatic hyperplasia (BPH) and prostate cancer in the transitional zone using magnetic resonance spectroscopic imaging ( MRSI), and to analyze the characteristics and differences of the spectra in this zonal area.Methods Eighteen patients with prostate cancer in the transitional zone underwent magnetic resonance imaging( MRI)/MRSI examinations. The ( Choline + Creatine)/Citrate (CC/Ci) ratio and the Choline/Creatine (Cho/Cr) ratio were evaluated in each voxel with cancer or BPH confirmed by pathological results. Discriminant analysis was used to determine the power of the two ratios in differentiation between cancer and BPH.Results The CC/Ci ratio and Cho/Cr ratio for cancer voxels were significantly higher than those in the voxels with BPH in the transitional zone ( CC/Ci: 2.36 ± 1.31 vs. 0.85 ± 0.29, P < 0.01; Cho/Cr: 4.14 ± 1.79 vs. 1.26 ±0.45, P < 0.01 ). As for the discriminant function with the CC/Ci ratio and the Cho/Cr ratio, the specificity, sensitivity, and accuracy were 98.6%, 85.7%, 92.9% respectively for the differentiation between cancer and BPH.Conclusions The prostate cancer is characterized by higher CC/Ci ratio and Cho/Cr ratio compared to BPH in the transitional zone. Both CC/Ci ratio and Cho/Cr ratio have high specificity, sensitivity, and accuracy in their discriminative power between cancer and BPH in this zonal area.

  12. Expressions of SIgA and α1-AR in benign prostatic hyperplasia combined with chronic prostatitis and their implications%良性前列腺增生合并慢性前列腺炎组织中SIgA、α1-AR的表达与意义

    Institute of Scientific and Technical Information of China (English)

    高卫军; 王养民; 王长海; 杨旭凯; 万霖; 李卫平

    2013-01-01

    目的:了解良性前列腺增生(BPH)合并慢性前列腺炎(CP)组织中SIgA、α1-AR的表达及意义.方法:将62例行经尿道前列腺等离子电切组织标本,根据术前前列腺按摩液(EPS)常规、经直肠前列腺B超、血清前列腺特异抗原(PSA)、国际前列腺症状评分(IPSS)、临床症状以及有无慢性骨盆疼痛综合征以及术后组织病理检查结果分为单纯BPH、BPH合并慢性前列腺炎两组.采用免疫组化、RT-PCR等实验方法研究两组前列腺组织中SIgA、α1-AR的表达. 结果:62例患者中30例为BPH合并慢性前列腺炎,32例为单纯BPH,通过对患者一般资料对比和对实验结果进行统计学分析,合并慢性前列腺炎的BPH患者SIgA、α1-AR表达显著高于单纯BPH患者(0.380 8±0.144 3 vs0.295 4±0.008 4;0.440 5±0.104 1 vs 0.383 2±0.013 6),差异有统计学意义(P<0.05).结论:SIgA、α1-AR在BPH合并慢性前列腺炎患者中的表达上调,提示慢性前列腺炎与BPH可能有一定的内在联系,炎症反应可能是BPH的一个致病因素,与BPH的病理发展过程有关.%Objective: To explore the expressions of SIgA and al-AR in benign prostatic hyperplasia (BPH) complicated by chronic prostatitis (CP) and their implications. Methods; According to the preoperative findings of expressed prostatic secretion (EPS) , transrectal prostate ultrasonography, prostate-specific antigen (PSA) , international prostate symptom score (IPSS) , clinical symptoms, chronic pelvic pain syndrome ( CPPS) and postoperative histopathology, 62 cases of BPH pathologically confirmed after transurethal plasmakinetic resection of the prostate (PKRP) were divided into a BPH group (n = 32) and a BPH + CP group (n = 30). The expressions of SIgA and al-AR in the prostate tissue were determined by immunohistochemistry and PT-PCR. Results; Of the 62 cases, 30 were found to be BPH + CP, and the other 32 to be BPH. The expressions of SIgA and al-AR were significantly higher in the

  13. Research of the correlation between obesity and benign prostatic hyperplasia%肥胖与良性前列腺增生的相关性研究

    Institute of Scientific and Technical Information of China (English)

    张杰; 潘永军; 黄捷; 陈雄; 张雄

    2016-01-01

    Objective To investigate the correlation between obesity and benign prostatic hyperplasia (BPH) ,and explore the pathogenesis of BPH .Methods A total of 120 patients with BPH were collected and divided into the normal group ,overweight group and obesity group according to the BMI .Indexs of fasting blood glucose (FBG) ,triglyceride(TG) ,high density lipoprotein cholesterol (HDL-C) ,interleukin 6 (IL-6) ,prostate volume(PV ) ,maximal urinary flow rate (Qmax ) and international prostate symptom score (IPSS) .And the correlation between obesity and BPH indexes were analyzed by statistical method .Results In the overweight group and obesity group ,the level of IPSS ,PV and IL-6 were significantly higher than those in the normal group (P<0 .05) .The score of Qmax was significantly lower than that in the normal group (P< 0 .05) .The study revealed a positive correla-tion between IPSS ,PV ,IL-6 and BMI (r= 0 .486 ,0 .518 and 0 .729 ,P< 0 .01) .And Qmax was negatively correlated with BMI (r=- 0 .391 ,P< 0 .01) .Conclusion Obesity is closely correlated with the occurrence and development of BPH ,IL-6 may play an im-portant role in the pathological and physiological process of BPH .%目的:分析肥胖与良性前列腺增生(BPH)的临床相关性,并对 BPH 的发病机制进行初步探讨。方法选择在该院泌尿外科住院的 BPH 患者120例,收集患者的体质量指数(BMI),空腹血糖(FBG)、三酰甘油(TG)、高密度脂蛋白胆固醇(HDL-C)、白介素6(IL-6)、前列腺体积(PV)、最大尿流率(Qmax)、国际前列腺症状评分(IPSS)等临床资料。按 BMI 将其分为正常组、超体质量组和肥胖组,比较分析各组中 BPH 患者各指标的差异及相关性。结果与正常组 BPH 患者比较,超体质量组和肥胖组的 IPSS 、PV 、IL-6升高,差异有统计学意义(P<0.05),而超体质量组和肥胖组的 Qmax 降低,差异有统计学意义(P<0.05);120

  14. 组织学前列腺炎对前列腺增生临床进展的影响因素分析%Analysis of the Effect of Histological Prostatitis on the Clinical Development of Prostatic Hyperplasia

    Institute of Scientific and Technical Information of China (English)

    吴自余; 马松; 王洪兵; 姜福金; 李强

    2011-01-01

    [目的]探讨组织学前列腺炎对良性前列腺增生(BPH)临床进展的影响.[方法]对手术治疗的143例BPH患者的临床资料进行回顾性分析,根据术后病理学检查前列腺炎的发生情况,分为单纯BPH组(n=35)和伴前列腺炎的BPH组(n=108),对比两组患者的发病年龄,前列腺体积,尿潴留发生率,国际前列腺症状评分(IPSS),最大尿流率 (MFR)和血清前列腺特异抗原(PSA)的数值.[结果]在所选前列腺标本中,75.6%(108/143)的标本同时合并组织学前列腺炎.伴组织学前列腺炎的BPH组患者与单纯BPH组相比其发病年龄、前列腺体积及血清PSA值明显升高(P<0.05),而且其急性尿潴留的发生率要大于单纯BPH组(P<0.05).[结论]BPH患者组织学前列腺炎的检出率较高,组织学前列腺炎明显影响BPH的临床进展,其可能为BPH的致病因素之一.%[Objective] To explore the effect of histological prostatitis on the development of benign prostatic hyperplasia(BPH). [Methods] The clinical data of 143 BPH patients treated by surgery were analyzed retrospectively. According to the incidence of prostatitis by postoperative pathological examination, all patients were divided into simple BPH group( n=35) and BPH with prostatitis group( n=108). The onset age, prostate volume, urlnary retention rate, IPSS, maximum flow rate(MFR) and serum prostate specific antigen(PSA) were compared between two groups. [Results] BPH combined with histological prostatitis occupied 75.6 % (108/143) of all prostate specimens. Compared with simple BPH group, the onset age, prostate volume and serum PSA in BPH with histological prostatitis group obviously increased( P <0.05). Moreover, the incidence of acute urinary retention in BPH with histological prostates was higher than that in simple BPH group( P <0.05). [Conclusion] The detection rate of histological prostatitis in BPH patients is higher. Histological prostatitis has obvious effect on the clinical development

  15. Prostatic Inflammation is Determinant for Prostate Overgrowth and Luts Severity in Men with Metabolic Syndrome: Highlights from Two Recently Published Multicentre Studies

    Directory of Open Access Journals (Sweden)

    Mauro Gacci

    2013-12-01

    Full Text Available Introduction: Several evidences have pointed out the possible association between Metabolic Syndrome (MetS and low urinary tract symptoms (LUTS/benign prostate hyperplasia (BPH. Recent epidemiological and histopatological evidences suggested chronic inflammation is a crucial event in BPH pathogenesis. Aim of this study is to demonstrate the correlation among pre-operatory LUTS/BPH severity, MetS features and inflammatory infiltrates in prostatectomy specimens of patients with BPH, highlighting the results of two recently published multicentre studies analyzing all the data from a preclinical and clinical point of view. Materials and methods: We conducted two retrospective study in 271 and 244 consecutive men treated with simple prostatectomy for LUTS/BPH in two tertiary referral centres. Prostate diameters and volume were measured by transrectal ultrasound, LUTS were scored by IPSS, and obstruction diagnosed by uroflowmetry. MetS was defined according to DF & AHA/NHLBI criteria. The inflammatory infiltrate was investigated according to the scoring system of chronic prostatitis (CP-CPPS and scored as inflammation score (IS ranging 3 to 9 and glandular disruption (GD. In addition, we investigated the in vitro inflammatory effects of metabolic insults on human prostatic myofibroblast cells isolated from BPH patients (hBPH. Results: Of 271 men, 86 (31.7% were affected by MetS. Prostatic volume and the anterior-posterior (AP diameter were positively associated to the number of MetS components. Among MetS determinants, only dyslipidaemia (increased serum triglycerides and reduced serum HDL levels was significantly associated with an increased risk of having a prostatic volume >60cm3. IS in prostatectomy specimens showed a step- wise association with number of MetS factors (p=0.001. Dyslipidaemia was the only factor significantly associated with IS. Positive significant correlations among MetS, IS, GD and IPSS Scores were observed. In myofibroblastic hBPH

  16. Reactions of excited-state benzophenone ketyl radical in a room-temperature ionic liquid.

    Science.gov (United States)

    Takahashi, Kenji; Tezuka, Hiroaki; Kitamura, Shingo; Satoh, Toshifumi; Katoh, Ryuzi

    2010-02-28

    The photochemistry of the benzophenone ketyl radical in D(1) excited state, BPH(D(1)), was studied by means of two-color dual-pulse laser flash photolysis (355 and 532 nm) in a room-temperature ionic liquid, 1-butyl-3-methylimidazolium bis(trifluoromethylsulfonyl)amide (Bmim-TFSA), and in methanol. Upon excitation with the 532 nm pulse, BPH(D(1)) emitted strong fluorescence. The transient absorption and fluorescence spectra of BPH(D(1)) were measured with nanosecond and sub-nanosecond time resolution, respectively. The observed Stokes shift was 1700 cm(-1) in Bmim-TFSA, and this shift was close to that in acetonitrile. The fluorescence lifetime of BPH(D(1)) was determined to be 5 ns in Bmim-TFSA, and again the value was close to that in acetonitrile. The rate constant of the reaction of BPH(D(1)) with CCl(4) in Bmim-TFSA was determined to be (2.1 +/- 0.4) x 10(9) M(-1) s(-1), which was 10 times the rate constant calculated on the basis of the bulk viscosity of Bmim-TFSA. The results are discussed in terms of the effective microscopic viscosity of the ionic liquid that was recently reported for the cage effect.

  17. Presence of PSA auto-antibodies in men with prostate abnormalities (prostate cancer/benign prostatic hyperplasia/prostatitis).

    Science.gov (United States)

    Lokant, M T; Naz, R K

    2015-04-01

    Prostate-specific antigen (PSA), produced by the prostate, liquefies post-ejaculate semen. PSA is detected in semen and blood. Increased circulating PSA levels indicate prostate abnormality [prostate cancer (PC), benign prostatic hyperplasia (BPH), prostatitis (PTIS)], with variance among individuals. As the prostate has been proposed as an immune organ, we hypothesise that variation in PSA levels among men may be due to presence of auto-antibodies against PSA. Sera from healthy men (n = 28) and men having prostatitis (n = 25), BPH (n = 30) or PC (n = 29) were tested for PSA antibody presence using enzyme-linked immunosorbent assay (ELISA) values converted to standard deviation (SD) units, and Western blotting. Taking ≥2 SD units as cut-off for positive immunoreactivity, 0% of normal men, 0% with prostatitis, 33% with BPH and 3.45% with PC demonstrated PSA antibodies. One-way analysis of variance (anova) performed on the mean absorbance values and SD units of each group showed BPH as significantly different (P prostatitis. All others were nonsignificant (P prostate abnormalities, especially differentiating BPH from prostate cancer and prostatitis.

  18. Effectiveness of a nonsteroidal anti-inflammatory drug for nocturia on patients with benign prostatic hyperplasia: a prospective non-randomized study of loxoprofen sodium 60 mg once daily before sleeping.

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    Araki T

    2004-02-01

    Full Text Available We explored the effectiveness of loxoprofen sodium (loxoprofen, which is the most common non-steroidal anti-inflammatory drug (NSAID in Japan, for patients with benign prostatic hyperplasia (BPH complaining of nocturia. A total of 93 BPH patients aged 49-84 years were enrolled in the study. These patients had received standard drug therapy with alpha1-blocker for BPH, followed by anticholinergic drugs, hypnotics, tricyclic antidepressants, and/or antiduretic hormone, but they still complained about 2 or more episodes of nocturia. They each took a single 60-mg tablet of loxoprofen prior to sleeping at night for 14 days in addition to their BPH treatments. The effects were assessed by questionnaire before and after treatment as excellent (nocturia disappeared or decreased by 2 or more voids/night, improved (nocturia decreased by 1 void/night, unchanged, or worsened (nocturia increased. Nocturia improved or disappeared in 74.2% of patients: excellent, improved, unchanged, and worsened results were obtained in 37.6%, 36.6%, 21.5%, and 4.3% of patients, respectively. The effects were better in patients whose baseline nocturia was > 2 times than in those with a lesser frequency at enrollment (P = 0.04. Loxoprofen can be an effective and useful treatment option for patients with BPH complaining of refractory nocturia.

  19. Ultrasonographic Findings of the Prostatic Disease : Comparative Analysis of the Benign and Malignant Nodules

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    Song, Yun Gyu; Kim, Ji Yang; Lee, Su Han; Kong, Su Jin; Sung, Young Soon; Kwon, Jae Soo [Masan Samsung General Hospital, Masan (Korea, Republic of)

    1996-06-15

    We evaluated the characteristics of the benign and malignant nodules on transrectal ultrasound in diagnosis of prostatic disease. Histologic examination of the trans perineal prostatic biopsy of the total 47 cases resulted in 19 cases of BPH, 8 cases of prostatic cancer, and 20 cases of normal prostatic tissue group. The hypoechoic mass in peripheral zone on TRUS had high possibility of prostatic carcinoma and the isoechoic or mixed echogenic mass in central gland had high possibility of benign lesion. Hypoechoic haloes around nodules and cysts were noted in BPH and normal prostatic tissue group, that were compatible with benign lesion. The mean value of PSA was 12.0 ng/ ml in BPH, 8.5 ng / ml in normal prostatic tissue group, and 65.6 ng / ml in prostatic cancer, which was very high in prostatic cancer. Between BPH and normal prostatic tissue group, there was no demonstrable difference in location of nodule, pattern of calcification, and echogenicity of the nodules on TRUS. The size of prostatic gland was relatively smaller and mean value of PSA was lower in normal prostatic tissue group, compared with in BPH. In conclusion, the location of the nodules and PSA value are considered to be important in differentiation of the benign and malignant prostatic nodules

  20. Pharmacological effects of saw palmetto extract in the lower urinary tract

    Institute of Scientific and Technical Information of China (English)

    Mayumi SUZUKI; Yoshihiko ITO; Tomomi FUJINO; Masayuki ABE; Satomi ONOUE; Keizo UMEGAKI; Hiroshi NOGUCHI; Shizuo YAMADA

    2009-01-01

    Saw palmetto extract (SPE), an extract from the ripe berries of the American dwarf palm, has been widely used as a thera-peutic remedy for urinary dysfunction due to benign prostatic hyperplasia (BPH) in Europe. Numerous mechanisms of action have been proposed for SPE, including the inhibition of 5α-reductase. Today, α1-adrenoceptor antagonists and muscarinic cholinoceptor antagonists are commonly used in the treatment of men with voiding symptoms secondary to BPH. The improvement of voiding symptoms in patients taking SPE may arise from its binding to pharmacologically rel-evant receptors in the lower urinary tract, such as α1-adrenoceptors, muscarinic cholinoceptors, 1,4-dihyropyridine recep-tors and vanilloid receptors. Furthermore, oral administration of SPE has been shown to attenuate the up-regulation of α1-adrenoceptors in the rat prostate induced by testosterone. Thus, SPE at clinically relevant doses may exert a direct effect on the pharmacological receptors in the lower urinary tract, thereby improving urinary dysfunction in patients with BPH and an overactive bladder. SPE does not have interactions with co-administered drugs or serious adverse events in blood biochemical parameters, suggestive of its relative safety, even with long-term intake. Clinical trials (placebo-controlled and active-controlled trials) of SPE conducted in men with BPH were also reviewed. This review should contribute to the under-standing of the pharmacological effects of SPE in the treatment of patients with BPH and associated lower urinary tract symptoms (LUTS).

  1. Inhibitory effect of Coffea arabica bean in testosterone induced prostatic hyperplasia in Sprague-Dawley rats

    Directory of Open Access Journals (Sweden)

    Kristian Alfonso G. Cueto

    2016-05-01

    Full Text Available Benign prostatic hyperplasia (BPH has been described as the uncontrolled prostate gland growth which leads to difficulty in urination. One of the treatment of BPH is saw palmetto lipid extracts which has been shown to inhibit prostate 5 α-reductase and some of its components (lauric acid, myristic acid and oleic acid also inhibit the enzyme. Coffee was also rich in fatty acids namely linoleic acid, oleic acid and palmitic acid. The aim of this research is to investigate whether coffee is effective in preventing testosterone-induced prostatic hyperplasia in rats using testosterone propionate and estradiol valerate. After and before the induction, the rats were tested for prostate specific antigen (PSA . The condition of the prostate gland of the test animals were correlated with the results of the said test and in the histopathologic results. After 14 days of experimentation, animals in the test group significantly decreased their PSA levels as compared to the BPH group. The histomorphology showed that Coffea arabica bean oil inhibited testosterone propionate while estradiol valerate induced prostatic hyperplasia. These findings indicate that Coffee arabica bean oil effectively inhibited the development of BPH. With the proven safety of coffee oil, these findings strongly support the feasibility of using Coffea arabica bean oil therapeutically in treating BPH.

  2. Genome-wide screening for components of small interfering RNA (siRNA) and micro-RNA (miRNA) pathways in the brown planthopper, Nilaparvata lugens (Hemiptera: Delphacidae).

    Science.gov (United States)

    Xu, H-J; Chen, T; Ma, X-F; Xue, J; Pan, P-L; Zhang, X-C; Cheng, J-A; Zhang, C-X

    2013-12-01

    The brown planthopper (BPH), Nilaparvata lugens, is a major rice pest in Asia, and accumulated evidence indicates that this species is susceptible to RNA interference (RNAi); however, the mechanism underlying RNAi and parental RNAi has not yet been determined. We comprehensively investigated the repertoire of core genes involved in small interfering RNA (siRNA) and micro-RNA (miRNA) pathways in the BPH by comparing its newly assembled transcriptome and genome with those of Drosophila melanogaster, Tribolium castaneum and Caenorhabditis elegans. Our analysis showed that the BPH possesses one drosha and two Dicer (dcr) genes, three dsRNA-binding motif protein genes, two Argonaute (ago) genes, two Eri-1-like genes (eri-1), and a Sid-1-like gene (sid-1). Additionally, we report for first time that parental RNAi might occur in this species, and siRNA pathway and Sid-1 were required for high efficiency of systemic RNAi triggered by exogenous dsRNA. Furthermore, our results also demonstrated that the miRNA pathway was involved in BPH metamorphosis as depletion of the ago1 or dcr1 gene severely impaired ecdysis. The BPH might be a good model system to study the molecular mechanism of systemic RNAi in hemimetabolous insects, and RNAi has potential to be developed to control this pest in agricultural settings.

  3. Fibronectin Pattern in Benign Hyperplasia and Cancer of the Prostate

    Directory of Open Access Journals (Sweden)

    Miroslava M. Janković

    2008-01-01

    Full Text Available Fibronectin (FN is a multifunctional glycoprotein involved in cell-matrix interactions. It exhibits a complex pattern of forms differing in respect to aminoacid and oligosaccharide composition. In this study we examined glycobiochemical and functional properties of the FN in benign prostatic hyperplasia (BPH and prostatic cancer (PCa, attempting to resolve disease-related differences. Two BPH sera pools and three PCa sera pools were used as the FN source. The affinity-purified molecule was characterized by SDS-PAGE, immuno- and lectin blot, lectin-affinity chromatography and adhesion assay. BPH FN existed as intact molecule, giving the main immunoreactive band at 220 kDa. In contrast, PCa FN comprised three main immunoreactive fragments of 140, 110 and 90 kDa. As for glycosylation the ratio of altogether lectin-reactive PCa FN was different from that of BPH FN manifested as a decrease of Con A- and an increase of LCA-reactive moieties. Fibroblasts adhered to both FN preparations in a concentration dependent manner, but with a significantly lower efficiency to PCa FN. The results obtained showing distinct structural characteristics of PCa FN compared to BPH FN could be important for modulation of its ligand and recognition properties expressed as gain or loss of functions or as specific markers of its origin.

  4. Treating benign prostatic hyperplasia with botulinum neurotoxin.

    Science.gov (United States)

    Brisinda, G; Vanella, S; Marniga, G; Crocco, A; Maria, G

    2011-01-01

    Botulinum toxin (BoNT) has been increasingly used in the interventional treatment of several disorders; the use of this agent has extended to a plethora of conditions including focal dystonia, spasticity, inappropriate contraction in most gastrointestinal sphincters, eye movement disorders, hyperhidrosis, genitourinary disorders and aesthetically undesirable hyperfunctional facial lines. In addition, BoNT is being investigated for the control of pain, and for the management of tension or migraine headaches and myofascial pain syndrome. Benign prostatic hyperplasia (BPH) is a common condition in ageing men; the goal of therapy is to reduce the lower urinary tract symptoms (LUTS) associated with BPH and to improve the quality of life. However, medical treatment, including drugs that relax smooth muscle within the prostate and drugs that shrink the gland are not totally effective or without complications. The standard surgical treatment for BPH is progressively changing to minimally invasive therapies, but none of them has provided clear results. The use of BoNT-A to inhibit the autonomic efferent effects on prostate growth and contraction, and inhibit the abnormal afferent effects on prostate sensation, might be an alternative treatment for BPH. BoNT injections have several advantages over drugs and surgical therapies in the management of intractable or chronic disease; systemic pharmacologic effects are rare, permanent destruction of tissue does not occur, and graded degrees of relaxation may be achieved by varying the dose injected. In this paper, clinical experience over the last years with BoNT in BPH impaired patients will be illustrated.

  5. Immunohistochemical study of the expression of epidermal growth factor receptor in benign prostatic hypertrophy, prostatic intraepithelial neoplasia and prostatic carcinoma.

    Science.gov (United States)

    Rana, Shveta; Sen, Rajeev; Kalra, Rajnish; Arora, B; Sharma, Puja; Gahlawat, Sapna

    2006-10-01

    The present study provides an analysis of immunohistochemical expression and localization of epidermal growth factor receptor (EGFR) in formalin fixed paraffin embedded specimens of prostate. Thirty-five cases each of benign prostatic hypertrophy (BPH) and prostatic carcinoma and 30 cases of prostatic intraepithelial neoplasia (PIN) were taken up for study. Streptavidin biotin peroxidase method was employed for immunohistochemical staining. EGFR positivity was observed in all the cases (100%) of BPH and PIN and in only 10 cases (28.5%) of prostatic carcinoma. In both BPH and PIN the basal cells revealed significantly higher intensity and percentage cell positivity than the luminal cells. Intensity and percentage of positively stained basal cells in BPH was higher than PIN basal cells but the difference was not statistically significant. The intensity and percentage cell positivity of BPH basal cells and PIN basal and luminal cells were significantly greater than the epithelial cells of prostatic carcinoma. Presently, the significance of variable expression of EGFR in various types of prostatic lesions is unknown.

  6. Benign prostatic hyperplasia: An overview of existing treatment

    Directory of Open Access Journals (Sweden)

    Neelima Dhingra

    2011-01-01

    Full Text Available Benign prostatic hyperplasia (BPH is the most common condition in aging men, associated with lower urinary tract symptoms (LUTS. A better understanding of the prostate physiology, function, and pathogenesis has led to the development of promising agents, useful in the management of LUTS in men. The specific approach used to treat BPH depends upon number of factors like age, prostrate size, weight, prostate-specific antigen level, and severity of the symptoms. 5α-reductase inhibitors decrease the production of dihydrotestosterone within the prostate, which results in decreased prostate volume, increased peak urinary flow rate, improvement of symptoms, decreased risk of acute urinary retention, and need for surgical intervention. α1 -adrenergic receptor (α1 -AR antagonists decrease LUTS and increase urinary flow rates in men with symptomatic BPH, but do not reduce the long-term risk of urinary retention or need for surgical intervention. Clinical efficacy of either 5α-reductase inhibitor or α1 -AR antagonist has been further improved by using combination therapy; however, long-term outcomes are still awaited. Many more potential new therapies are under development that may improve the treatment of BPH. This article gives a brief account of rationale and efficacy of different treatment options presently available in the management of BPH.

  7. Mapping of a new gene for brown planthopper resistance in cultivated rice introgressed from Oryza eichingeri

    Institute of Scientific and Technical Information of China (English)

    2001-01-01

    Wild rice species is an important source of useful genes for cultivated rice improvement. Some accessions of Oryza eichingeri (2n = 24, CC) from Africa confer strong resistance to brown planthopper (BPH), whitebacked planthopper (WBPH) and bacterial blight (BB). In the present study, restriction fragments length polymorphism (RFLP) and simple sequence repeats (SSR) analysis were performed on disomic backcross plants between Oryza sativa (2n = 24, AA) and O. eichingeri in order to identify the presenee of O. eichingeri segments and further to localize BPH-resistant gene. In the introgression lines, 1-6O. eichingeri segments were detected on rice chromosomes 1, 2, 6, or/and 10. The dominant BPH resistant gene, tentatively named Bph13(t), was mapped to chromosome 2, being 6.1 and 5.5 cM away from two microsatellite markers RM240 and RM250, respectively. The transfer and localization of this gene from O. eichingeri will contribute to the improvement of BPH resistance in cultivated rice.``

  8. Environmental Exposure to Lead as a Risk for Prostate Cancer

    Institute of Scientific and Technical Information of China (English)

    2002-01-01

    Objective To evaluate the possible role of environmental exposure to lead as a risk factor for prostate pathology in patients suffering from prostate cancer (PCA) and benign prostate hyperplasia (BPH). Methods Blood lead (BPb) level was determined in PCA and BPH cases using a graphite furnace Atomic Absorption Spectrophotometer and compared with those in a control group living in the similar socioeconomic environment. Results BPb was significantly higher in PCA and BPH cases than in normals (P<0.05). Blood levels of zinc and copper were significantly lower in PCA and BPH cases when compared with controls (P<0.05). In all the three groups, a statistically significant positive correlation between lead and thiobarbituric acid reactive substances (TBARS) measured as malondialdehyde, and negative correlation between blood lead and antioxidant GSH level, indicative of possible generation of reactive oxygen species, were also observed after adjusting for age as a possible confounders. However, positive association between blood lead and TBARS was relatively higher in PCA patients (r=0.77, P<0.05) than in BPH (r=0.32, P<0.05) and normal (r=0.30, P<0.05).Conclusion These results with limited power seem to suggest for the first time that environmental exposure of aging males to lead may be a risk factor for prostate cancer and/or benign prostate hyperplasia possibly through generation of reactive oxygen species and/or reducing the level of zinc which acts as a cellular growth protector.

  9. A New Class of Organic Luminophores With a stilbene Chromophore: 3-Phenylmethylene-1(3-H) Isobenzofuranones

    Science.gov (United States)

    Nikolov, Peter; Fratev, Filip; Minchev, Stoyan

    1983-02-01

    A new class of luminophores with a stilbene chromophore, 3-phenylmethylene-1(3H)-iso-benzofuranones (BPH's), has been investigated. The fluorescence occurs in the region 26000-16000 cm-1, the maximal quantum yield being about 0.6. As a result of substitution or higher polarity of the solvent the ππ* state of the BPH's separates from the fluorescently inactive nπ* state. The S0-S2 absorption transition of the BPH's results from an excitation which is practically localized in the stilbene fragment. The good linear correlation of the fluorescence and absorption maxima and O-O transitions in ethanol with the σp-Hammett constants has been used for an interpretation of the changes in the potential hyperfaces of S0 and S1 states.

  10. Ablation of benign prostatic hyperplasia using microbubble-mediated ultrasound cavitation.

    Science.gov (United States)

    Li, Tao; Liu, Zheng

    2010-04-01

    Benign prostatic hyperplasia (BPH) is a world-wide common disease in elderly male patients. A number of invasive physiotherapies have been used to replace prostatectomy. In this article we report our hypothesis of using microbubbles-mediated ultrasound cavitation effects to ablate prostatic tissues. Microbubble ultrasound contrast agent is widely used contrast media in ultrasonography, yet it is also found to act as cavitation nuclei or enhancer. Once excited by a high peak pressure ultrasound pulse, the mechanical effects, like shock wave and microstream, released from cavitation could produce a series of bioeffects, contributing to sonoporation, microvascular rupture and hematoma. BPH is known to have hyperplastic neovasculature and this make it possible to be disrupted by the physical effects of cavitation under existing microbubbles in circulation. Mechanical ablation of prostatic capillary or small vessels could result in pathological alterations such as thrombosis, micro-circulation blockage, prostatic necrosis and atrophia. Thereupon it could effectively treat BPH by nontraumatic ways.

  11. NF-κB and androgen receptor variant 7 induce expression of SRD5A isoforms and confer 5ARI resistance

    DEFF Research Database (Denmark)

    Austin, David C; Strand, Douglas W; Love, Harold L;

    2016-01-01

    BACKGROUND: Benign prostatic hyperplasia (BPH) is treated with 5α-reductase inhibitors (5ARI). These drugs inhibit the conversion of testosterone to dihydrotestosterone resulting in apoptosis and prostate shrinkage. Most patients initially respond to 5ARIs; however, failure is common especially...... tract symptoms secondary to advanced BPH; and, cancer free transition zone from "Incidental" patients treated for low grade, localized peripheral zone prostate cancer. Clinical, molecular and histopathological profiles were analyzed. Human prostatic stromal and epithelial cell lines were genetically...... modified to regulate NF-κB activity, androgen receptor (AR) full length (AR-FL), and AR variant 7 (AR-V7) expression. RESULTS: SRD5A2 is upregulated in advanced BPH. SRD5A2 was significantly associated with prostate volume determined by Transrectal Ultrasound (TRUS), and with more severe lower urinary...

  12. The role of chronic prostatitis in the pathogenesis and progression of benign prostatic hyperplasia%慢性前列腺炎在前列腺增生症发病与进展中的作用探讨

    Institute of Scientific and Technical Information of China (English)

    张益明; 范武林; 林国太; 林永平; 陈如

    2016-01-01

    目的 探讨慢性前列腺炎在前列腺增生症发病与进展中的可能作用.方法 回顾性分析本院2011年5月至2014年12月期间因前列腺增生就诊患者356例,根据术后病理结果分为前列腺增生并慢性前列腺炎组及单纯性前列腺增生组,统计分析两组临床特征,包括:年龄(Age)、前列腺体积(PV)、PSA、IPSS评分、是否合并急性尿潴留(AUR).结果 前列腺增生并慢性前列腺炎121/356例(34.0%),发生急性尿潴留48/121例(39.7%);单纯前列腺增生235/356例(66.0%),发生尿潴留60/235例(25.5%).两组对比年龄差别无统计学意义(P>0.05);但前列腺增生合并慢性前列腺炎组较单纯前列腺增生组PV、PSA、IPSS评分、尿潴留发生率均高,差别具有统计学意义(P<0.05).结论 前列腺增生合并慢性前列腺炎通常具有更大的体积、更高的PSA及IPSS评分、更易发生尿潴留.因此,前列腺慢性炎症在前列腺增生的发病、进展中可能起作用.%Objectives To investigate the role of chronic prostatic inflammation in the pathogenesis and progression of benign prostatic hyperplasia (BPH).Methods We enrolled 356 patients with BPH from May 2011 to December 2014 in our hospital were analyzed retrospectively.According to the pathological results,all patients were divided into the BPH with prostatitis group and BPH group.Age,prostate volume (PV),PSA,IPSS,and acute urinary retention (AUR) of the two groups were analyzed.Results In the BPH with prostatitis group,121/356 (34.0%) patients were be confirmed as BPH with prostatitis,the other 48/121 (39.7%) patients were be confirmed as acute urinary retention (AUR).In the BPH group,235/356 (66.0%) patients were be comfirmed as simple BPH.and there were 60 (25.5%) patients with AUR.There was no significant difference in patient's age between the two groups (P > 0.05),but PV,PSA,IPSS and incidence of AUR in the BPH with prostatitis group were higher than BPH group (P

  13. Upregulation of Talin-1 expression associates with advanced pathological features and predicts lymph node metastases and biochemical recurrence of prostate cancer

    Science.gov (United States)

    Xu, Ning; Chen, Hui-Jun; Chen, Shao-Hao; Xue, Xue-Yi; Chen, Hong; Zheng, Qing-Shui; Wei, Yong; Li, Xiao-Dong; Huang, Jin-Bei; Cai, Hai; Sun, Xiong-Lin

    2016-01-01

    Abstract Talin-1 functions to regulate cell–cell adhesion, and its altered expression was reported to be associated with human carcinogenesis. A total of 280 tissue specimens from prostate cancer (PCa) patients who underwent radical prostatectomy, 75 cases of benign prostatic hyperplasia (BPH) tissue, and 6 cases of normal prostate tissue specimens were collected for construction of tissue microarray and subsequently subjected to immunohistochemical staining of Talin-1 expression. Talin-1 expression was significantly higher in PCa than both normal and BPH tissues (P BPH tissue and Talin-1 expression was an independent predictor for lymph node metastasis and biochemical recurrence of PCa. Further study will investigate the underlying molecular mechanism and the role of Talin-1 in PCa. PMID:27442684

  14. {sup 90}Sr/{sup 90}Y prostatic hyperplasia applicators and its clinical effects

    Energy Technology Data Exchange (ETDEWEB)

    Shanyu Cai [Academia Sinica, Beijing, BJ (China). Inst. of Atomic Energy; Zhi Li; Feng Chen

    1996-10-01

    Several main techniques for the treatment of benign prostatic hyperplasia (BPH) are reviewed simply in this paper. A novel technique of {sup 90}Sr/{sup 90}Y intracavitary therapy is described in detail. In recent years, two different kinds of {sup 90}Sr/{sup 90}Y prostatic hyperplasia applicators including `urethra-type` and `rectum-type` have been developed. The hypertrophic prostate gland can be irradiated by {sup 90}Sr/{sup 90}Y ({beta}) rays through the wall of urethra or rectum. Based on the animal experiments, a thousand cases of BPH have been observed respectively at ten hospitals since 1992. The clinical application indicate that the {sup 90}Sr/{sup 90}Y intracavitary applicator provides a safe, effective, reliable, and non-invasive method in curing BPH. In addition, it is apt for the high risk patients and costs of treatment are low. (author)

  15. Tai Chi for Lower Urinary Tract Symptoms and Quality of Life in Elderly Patients with Benign Prostate Hypertrophy: A Randomized Controlled Trial

    Directory of Open Access Journals (Sweden)

    Seil Jung

    2012-01-01

    Full Text Available Tai chi exercise has been recommended as suitable for the improvement of health in the elderly. The purpose of this study was to investigate the effects of tai chi on lower urinary tract symptoms (LUTSs, quality of life (QoL, and sex hormone levels in patients with benign prostate hypertrophy (BPH. The elderly patients with BPH were randomized to receive tai chi or usual care. Fifty-six participants were randomized into either the tai chi group (n=28 or the control group (n=28. After 12 weeks of treatment, the tai chi group showed significant improvement in LUTS and QoL. There was a significant effect of tai chi on testosterone but no significant effect on insulin or glucose. No serious adverse events were observed during the study period. In conclusion, our results suggest that 12 weeks of tai chi may improve LUTS and QoL in elderly patients with BPH.

  16. Transurethral prostatectomy.

    Science.gov (United States)

    Holtgrewe, H L

    1995-05-01

    In properly selected patients, TURP has the highest probability of symptom relief of any strategy of BPH management except open prostatectomy, the outcomes of which are but marginally better. However, open prostatectomy suffers from lack of patient acceptance, increased postoperative discomfort, and prolonged hospitalization, factors which over the past six decades relegated it to a minor role in the surgical management of BPH. Given the advanced age and compromised health status of most men undergoing TURP, the procedure's current mortality, morbidity, and long-term complication rates are remarkable. The ultimate role of the emerging alternative strategies of BPH management--hormonal, pharmacologic, thermal, and device discussed elsewhere in this issue, will be dependent upon their durability and their long-term outcomes, which remain to be fully defined. In the meantime, TURP remains the standard against which all new therapies must be measured. The resectoscope is being challenged but is not yet ready for the history books.

  17. Prostate Artery Embolization as a New Treatment for Benign Prostate Hyperplasia: Contemporary Status in 2016.

    Science.gov (United States)

    Noor, Amir; Fischman, Aaron M

    2016-07-01

    The gold standard treatment for benign prostate hyperplasia (BPH) is transurethral resection of the prostate (TURP) or open prostatectomy (OP). Recently, there has been increased interest and research in less invasive alternative treatments with less morbidity including prostate artery embolization (PAE). Several studies have shown PAE to be an effective alternative to TURP to treat lower urinary tract symptoms (LUTS) associated with BPH with decreased morbidity. Specifically, PAE has been advantageous in selected patient populations such as those with prostates too large for TURP or unsuitable surgical candidates, showing a promising potential for the future care of patients with BPH. Further studies are being done to demonstrate the clinical applications and advantages of this therapy in reduction of LUTS.

  18. Expression of cyclooxygenase-2 in prostate adenocarcinoma and benign prostatic hyperplasia.

    Science.gov (United States)

    Lee, L M; Pan, C C; Cheng, C J; Chi, C W; Liu, T Y

    2001-01-01

    Elevated expression of cyclo-oxygenase (COX)-2 has been found in several human cancers, including prostate adenocarcinoma. To evaluate the potential prognostic role of COX-2 in prostate cancer, we assessed the expression of COX-2 in benign prostatic hyperplasia (BPH) and prostate cancer samples employing immunohistochemistry. COX-2 was over-expressed in 15 out of 18 (83%) prostate cancer samples whereas it was detected in only 22% (4 of 18) paired benign tissues. The intensity of immunostaining correlated with the tumor grading. In addition, COX-2 was expressed in 7 of the 22 (32%) BPH samples examined. The significance a COX-2 expression in the BPH samples is not known at present. This data suggest that COX-2 is over-expressed in prostate cancer and COX-2 inhibitors may be useful in combination chemotherapy or chemoprevention for prostate cancer.

  19. Spectrum of histological lesions in 185 consecutive prostatic specimens.

    Directory of Open Access Journals (Sweden)

    Mittal B

    1989-07-01

    Full Text Available One hundred and eighty five consecutive prostate specimens were studied. The predominant lesion noted was benign prostatic hyperplasia (B.P.H. (92.97%. The incidence of carcinoma was low, (7.02%. Conditions which can mimic and should be considered in the differential diagnosis of carcinoma, like basal cell hyperplasia, atypical hyperplasia and atrophy associated hyperplasia were noted in 10, 4 and 3 cases of B.P.H. respectively. None of these cases showed evidence of carcinoma. Corpora amylacea were noted in 38.91% of the cases of B.P.H. and were conspicuously absent in cases of carcinoma. Chronic prostatitis was frequently encountered (58% and metaplastic changes were seen in 11% of the cases.

  20. Study of human prostate spheroids treated with zinc using X-ray microfluorescence

    Energy Technology Data Exchange (ETDEWEB)

    Leitao, Roberta G.; Lopes, Ricardo T.; Pereira, Gabriela R., E-mail: roberta@lin.ufrj.br, E-mail: gpereira@metalmat.ufrj.br [Coordenacao dos Cursos de Pos-Graduacao em Engenharia (COPPE/UFRJ), Rio de Janeiro, RJ (Brazil); Santos, Carlos A.N., E-mail: cansantos.bio@gmail.com [Instituto Nacional de Metrologia, Qualidade e Tecnologia (DIPRO/INMETRO), Duque de Caxias, RJ (Brazil). Lab. de Biotecnologia; Palumbo Junior, Antonio; Nasciutti, Luiz E., E-mail: nasciutt@ufrj.br [Universidade Federal do Rio de Janeiro (ICB/CCS/UFRJ), Rio de Janeiro, RJ (Brazil). Lab. de Interacoes Celulares; Souza, Pedro A.V.R., E-mail: pedroaugustoreis@uol.com.br [Hospital Federal do Andarai (HFA), Rio de Janeiro, RJ (Brazil). Servico de Urologia; Anjos, Marcelino J., E-mail: marcelin@lin.ufrj.br [Universidade Estatual do Rio de Janeiro (UERJ), Rio de Janeiro, RJ (Brazil). Inst. de Fisica

    2013-07-01

    Spheroids cell culture is a useful technique for tissue engineering or regenerative medicine re-search, pharmacological and toxicological studies, and fundamental studies in cell biology. In this study, we investigated Zn distribution in cell spheroids in benign prostate hyperplasia (BPH) and prostate cancer (DU145) and analyzed the differences in the response to Zinc (0-150 μM) treatment. The measurements were performed in standard geometry of 45 deg incidence, exciting with a white beam and using an optical capillary with 20 μm diameter collimation in the XRF beam line at the Synchrotron Light National Laboratory (Campinas, Brazil). The results showed non-uniform distribution of Zn in all the spheroids analyzed. The differential response to zinc of DU145 and BPH cell spheroids suggests that zinc may have an important role in prostate cancer and BPH diagnosis. (author)

  1. [A randomized comparative study assessing once versus twice a day treatment of benign prostatic hyperplasia with terazosin].

    Science.gov (United States)

    Suzuki, H; Ohnishi, T; Ikemoto, I; Ohishi, Y; Suzuki, Y; Yamazaki, H

    2001-02-01

    We compared the efficacy of once a day administration of terazosin hydrochloride with that of twice a day administration for benign prostatic hyperplasia (BPH) patients. Forty-two patients with BPH were randomly assigned to receive a maximum dose of 2 mg terazosin either once (n = 21) or twice (n = 21) a day. International prostate symptom score (IPSS), uroflowmetry and side effect profile were determined before and 4 weeks after randomization. Both groups were similar with respect to patient age, baseline IPSS and prostate volume. After 4 weeks of treatment with terazosin, significant improvement in IPSS, maximum flow rate and mean flow rate were observed in both groups. However, these improvements did not differ significantly between them. In addition, there were no differences in side effects between the groups. In conclusion, once a day administration of terazosin hydrochloride is as effective and safe as twice a day administration in patients with BPH.

  2. Abnormality of growth regulatory factors in benign prostatic hyperplasia%生长调控因子异常与前列腺增生的关系

    Institute of Scientific and Technical Information of China (English)

    艾力·艾尼; 史永华; 辛钟成

    2011-01-01

    目的 探讨多种生长调控因子在前列腺增生过程中的作用.方法 采用免疫组织化学Max vision二步法检测61例前列腺增生(BPH)和15例正常前列腺组织中TGF-β1、b-FGF、EGFR和TGF-α的表达.结果 TGF-β1主要定位于前列腺上皮细胞浆、间质细胞浆内,呈棕黄色颗粒,BPH及正常前列腺组织中上皮细胞阳性率分别为45.90%、13.33%;间质细胞阳性率分别为24.59%、6.67%,BPH中前腺上皮细胞和间质细胞总阳性率为52.46%,正常前列腺组织中上皮细胞和间质细胞总阳性率为20.00%,两组比较差异有统计学意义P0.05).结论 TGF-β1、b-FGF、EGFR和TGF-α是促进BPH的重要因素,TGF-β1、b-FGF和TGF-α主要与前列腺间质增生有关,EGFR主要与前列腺上皮增生有关.%Objective To investigate the role of 4 growth regulatory factors in the pathogenesis of benign prostatic hyperplasia (BPH). Methods The expressions of 4 growth regulatory factors were detected in 61 BPH tissues and 15 normal prostate tissues by Immunohistochemical Max vision staining. Results The expression rate of TGF β1 in the gland epithelium cell plasma and stroma cell plasma in BPH was 45.90% and 24.59% respectively, expressed in the BPH gland epithelium and stroma cell together 52.46%, and was 20.00% in normal prostatic tissue. The expression rate of b-FGF in in epithelium cell and the stroma cell plasma plasma in BPH was 26.23% and 75.41% respectively,expressed in the BPH gland epithelium and stroma cell together 75.41%, and was 13.33% in normal prostatic tissue.Expression of TGF β 1 and b-FGF in BPH was significantly higher than that in normal prostate tissue. EGFR was expressed mainly in the gland epithelium cell membrane in BPH, and TGF- α was expressed mainly in the stroma cell in BPH. Expressions of EGFR and TGF- α in BPH were significantly higher than those in normal prostate. Conclusion Abnormality of multiple growth regulatory factors might be involved in

  3. Management of Benign Prostatic Hyperplasia in Older Adults.

    Science.gov (United States)

    Woodard, Todd J; Manigault, Kendra R; McBurrows, Niesha N; Wray, Tiffany L; Woodard, Laresa M

    Benign prostatic hyperplasia (BPH), also known as benign prostatic hypertrophy, is a nonmalignant adenomatous overgrowth of the periurethral prostate gland commonly seen in aging men. Historically, it has been assumed that the pathophysiology of lower urinary tract symptoms in men is the result of bladder outlet obstruction associated with prostate enlargement. Symptoms such as urinary hesitancy, incomplete bladder emptying, dribbling or prolonged urination, nocturia, urinary urgency, and/or urge incontinence are common. Understanding the differential diagnosis and ordering appropriate laboratory tests are essential in accurately identifying a BPH diagnosis. Management can be broken down into medical or pharmacological and surgical therapies. This article aims to provide an overview of BPH and its management in older adults.

  4. Transient Occlusion of Bilateral Internal Iliac Arteries Facilitates Bloodless Operative Field in Subcapsular Prostatectomy

    Directory of Open Access Journals (Sweden)

    Takumi Takeuchi

    2012-01-01

    Full Text Available Transurethral resection of the prostate is the gold standard of surgical treatment for benign prostatic hyperplasia (BPH. Nevertheless, open subcapsular prostatectomy is still performed for large BPH. While enucleation of prostatic adenoma is being performed, unneglectable bleeding can occur and surgeons need to rush to remove adenomas, often using fingers and in a blinded fashion. The blood supply to the prostatic capsule and adenoma can be reduced to a marked extent in subcapsular prostatectomy if the bilateral internal iliac arteries are transiently occluded. Thus, a bloodless operative field is reasonably acquired during enucleation of adenoma, which would, otherwise, be a cause for concern to surgeons due to bleeding. It is not always applicable, but it could be an option if the estimated volume of BPH is more than 100 mL. In two cases, bilateral internal iliac arteries were occluded with Bulldog clamps, and then adenomas of 159 and 97 g were enucleated.

  5. Regulation of Prostate Development and Benign Prostatic Hyperplasia by Autocrine Cholinergic Signaling via Maintaining the Epithelial Progenitor Cells in Proliferating Status

    Directory of Open Access Journals (Sweden)

    Naitao Wang

    2016-05-01

    Full Text Available Regulation of prostate epithelial progenitor cells is important in prostate development and prostate diseases. Our previous study demonstrated a function of autocrine cholinergic signaling (ACS in promoting prostate cancer growth and castration resistance. However, whether or not such ACS also plays a role in prostate development is unknown. Here, we report that ACS promoted the proliferation and inhibited the differentiation of prostate epithelial progenitor cells in organotypic cultures. These results were confirmed by ex vivo lineage tracing assays and in vivo renal capsule recombination assays. Moreover, we found that M3 cholinergic receptor (CHRM3 was upregulated in a large subset of benign prostatic hyperplasia (BPH tissues compared with normal tissues. Activation of CHRM3 also promoted the proliferation of BPH cells. Together, our findings identify a role of ACS in maintaining prostate epithelial progenitor cells in the proliferating state, and blockade of ACS may have clinical implications for the management of BPH.

  6. Occurrence of Tn4371-related mobile elements and sequences in (chloro)biphenyl-degrading bacteria.

    Science.gov (United States)

    Springael, D; Ryngaert, A; Merlin, C; Toussaint, A; Mergeay, M

    2001-01-01

    Tn4371, a 55-kb transposable element involved in the degradation and biphenyl or 4-chlorobiphenyl identified in Ralstonia eutropha A5, displays a modular structure including a phage-like integrase gene (int), a Pseudomonas-like (chloro)biphenyl catabolic gene cluster (bph), and RP4- and Ti-plasmid-like transfer genes (trb) (C. Merlin, D. Springael, and A. Toussaint, Plasmid 41:40-54, 1999). Southern blot hybridization was used to examine the presence of different regions of Tn4371 in a collection of (chloro)biphenyl-degrading bacteria originating from different habitats and belonging to different bacterial genera. Tn4371-related sequences were never detected on endogenous plasmids. Although the gene probes containing only bph sequences hybridized to genomic DNA from most strains tested, a limited selection of strains, all beta-proteobacteria, displayed hybridization patterns similar to the Tn4371 bph cluster. Homology between Tn4371 and DNA of two of those strains, originating from the same area as strain A5, extended outside the catabolic genes and covered the putative transfer region of Tn4371. On the other hand, none of the (chloro)biphenyl degraders hybridized with the outer left part of Tn4371 containing the int gene. The bph catabolic determinant of the two strains displaying homology to the Tn4371 transfer genes and a third strain isolated from the A5 area could be mobilized to a R. eutropha recipient, after insertion into an endogenous or introduced IncP1 plasmid. The mobilized DNA of those strains included all Tn4371 homologous sequences previously identified in their genome. Our observations show that the bph genes present on Tn4371 are highly conserved between different (chloro)biphenyl-degrading hosts, isolated globally but belonging mainly to the beta-proteobacteria. On the other hand, Tn4371-related mobile elements carrying bph genes are apparently only found in isolates from the environment that provided the Tn4371-bearing isolate A5.

  7. Preventive effects of lignan extract from flax hulls on experimentally induced benign prostate hyperplasia.

    Science.gov (United States)

    Bisson, Jean-François; Hidalgo, Sophie; Simons, Rudy; Verbruggen, Marian

    2014-06-01

    Consumption of diet rich in lignans may decrease the risk of some chronic hormonal conditions such as benign prostatic hyperplasia (BPH). This study investigated whether a lignan-rich extract from flaxseed hulls, LinumLife EXTRA (LLE), could prevent BPH using the testosterone propionate (TP)-induced BPH rat model. Male Wistar-Unilever rats were randomly divided into four groups of 12 rats each: a negative control group fed with control diet and receiving daily subcutaneous injections of corn oil without TP, and three groups fed with control diet (positive control), diet containing 0.5% LLE (LLE 0.5) or 1.0% LLE (LLE 1.0) and receiving daily subcutaneous injections of TP in corn oil. Treatments with diets started 2 weeks before the induction of BPH and were carried out for 5 consecutive weeks. The influence of TP and LLE on body weight (BW), food and water consumptions, and enterolactone (ENL) levels in serum and urine of rats was examined at the end of the 5-week treatment period. TP significantly diminished the mean body weight gain (MBWG) of positive control rats and their food and water consumptions while LLE reduced significantly this MBWG reduction in a dose-dependent manner. The lignan-rich extract significantly inhibited TP-induced prostate size ratio (prostate weight/rat BW) increase in comparison with positive controls (P<.001). This effect was dose dependent. Higher serum and urine levels of ENL correlated well with the dose of extract provided to rats. It was concluded that the lignan-rich flaxseed hull extract prevented the TP-induced BPH indicating it might be beneficial in the prevention of BPH.

  8. Anthropogenic and naturally occurring polybrominated phenolic compounds in the blood of cetaceans stranded along Japanese coastal waters

    Energy Technology Data Exchange (ETDEWEB)

    Nomiyama, Kei, E-mail: keinomi@agr.ehime-u.ac.jp [Center for Marine Environmental Studies (CMES), Ehime University, Bunkyo-cho 2-5, Matsuyama 790-8577 (Japan); Eguchi, Akifumi; Mizukawa, Hazuki; Ochiai, Mari; Murata, Satoko; Someya, Masayuki [Center for Marine Environmental Studies (CMES), Ehime University, Bunkyo-cho 2-5, Matsuyama 790-8577 (Japan); Isobe, Tomohiko [Senior Research Fellow Center, Ehime University, Bunkyo-cho 2-5, Matsuyama 790-8577 (Japan); Yamada, Tadasu K. [Department of Zoology, National Museum of Nature and Science, 3-23-1 Hyakunin-cho, Shinjuku-ku, Tokyo 169-0073 (Japan); Tanabe, Shinsuke [Center for Marine Environmental Studies (CMES), Ehime University, Bunkyo-cho 2-5, Matsuyama 790-8577 (Japan)

    2011-12-15

    We determined the residue levels and patterns of hydroxylated polybrominated diphenyl ethers (OH-PBDEs), and related compounds, such as PBDEs, methoxylated PBDEs (MeO-PBDEs), and bromophenols (BPhs) in the blood of eleven cetacean species stranded along the Japanese coasts. The dominant OH- and MeO-PBDE isomers found in all cetaceans were 6OH-BDE47 and 6MeO-BDE47. Additionally, 2,4,6-triBPh was dominant isomer in all cetaceans. In contrast, specific differences in the distribution of para- and meta- OH-PBDE isomers and some BPhs (potential PBDEs metabolites) were found among the cetaceans. Residue levels of {Sigma}MeO-PBDEs and 6OH-BDE47 + 2'OH-BDE68, and 2,4,6-triBPh and 6OH-BDE47 + 2'OH-BDE68 showed a significant positive correlation. These results may suggest that the large percentages of OH-PBDEs, MeO-PBDEs and 2,4,6-triBPh might share common source (i.e. biosynthesis by marine organisms), or metabolic pathway in cetacean species. Significant correlations were found between the concentrations of BDE99 and 2,4,5-triBPh. This result suggested that 2,4,5-triBPh in cetaceans could be a metabolite of BDE99. - Highlights: > We determined the concentrations of OH-PBDEs in the blood of cetacean species. > OH-PBDEs, MeO-PBDEs and 2,4,6-triBPh showed a significant positive correlation. > It was presumed that large percentages of OH-PBDEs were the natural origins. > Specific differences in the distribution of PBDE metabolites were found in cetaceans. > Some bromophenols in cetaceans could be a metabolite of PBDEs. - Specific differences in the distribution of anthropogenic and naturally occurring polybrominated phenolic compounds and the relationships were found among the cetacean species.

  9. Cell Adhesion Regulates Expression of the Androgen Receptor and Coregulators in Different Prostate Cancer Cells

    Directory of Open Access Journals (Sweden)

    Sheng Li

    2007-02-01

    Full Text Available Prostate cancer cells adhere to a tumor basement membrane, while secretoryepithelial cells reside in a suprabasal cell compartment. Since tumor cells are derived fromsuprabasal epithelial cells, they experience de-novo substratum adhesion in the context ofoncogenesis. We therefore analyzed whether cell-matrix adhesion could affect the proteinexpression and activity of the AR. In this study, AR protein expression declined uponsuspension of BPH-1-AR cells, but not in PC-3-AR cells shown by Western blot. In a timecourse study, BPH-1 cell lost AR expression within 6 hours, and the synthetic androgen,R1881 reduced the loss of AR expression. We further explored the mechanism of AR loss insuspended BPH-1 cells. BPH-1-AR cells underwent apoptosis (anoikis when suspended for2 - 5 hours. Suspension did not induce significant apoptosis or decreasing of AR expressionin PC-3 cells. Inhibition of apoptosis in suspended BPH-1-AR cells, either by expression ofBcl-2 or Bcl-xl or by treatment with Z-VAD, a caspase inhibitor, prevented loss of ARprotein. In contrast, the calpain protease inhibitor , ALLN, accelerated the loss of AR proteinexpression. Additionally, cell-matrix adhesion changed the expression of coregulators of ARin the mRNA level of prostate cancer cells. Our results demonstrate that AR proteinexpression was reduced through activation of cell death pathways, and thus indirectly through cell suspension in BPH-AR cells. The activity of AR can also be regulated by adhesion in PC-3-AR and LNCaP cells through affecting the coregulators level.

  10. Influence of immune inflammation on androgen receptor expression in benign prostatic hyperplasia tissue

    Institute of Scientific and Technical Information of China (English)

    Zong-Lin Wu; Ya Yuan; He Geng; Shu-Jie Xia

    2012-01-01

    This study was designed to investigate the association between immune inflammation and androgen receptor (AR) expression in benign prostatic hyperplasia (BPH).We retrospectively analyzed 105 prostatectomy specimens.An immune inflammation score for each specimen was defined by combining three immunohistochemical markers (CD4,CD8 and CD20).The immunohistochemical markers were CD4 and CD8 for T lymphocytes,CD20 for B lymphocytes and AR antibody for the AR in BPH samples.Rates of CD4,CD8,CD20 and AR expression in BPH were 20 (19.0%),21 (20.0%),101 (96.2%) and 48 (45.7%),respectively.Total prostate volume (TPV) was higher in the immune inflammation group than in the non-immune inflammation group (62.7 ml vs.49.2 ml,t=2.482,P<0.05).Patients in the immune inflammation group had a higher serum prostate-specific antigen (PSA) than those in the non-inflammation group (7.5 ng ml-1 vs.5.4 ng ml-1,t=2.771,P<0.05).Specifically,the immune inflammation group showed a higher rate of AR expression than the non-inflammation group (56.1% vs.28.2%,x2=7.665,P<0.05).Our study revealed a strong association between immune inflammation and TPV,serum PSA and AR expression in BPH tissue.Prostate hyperplasia caused by an immune inflammatory process may contribute to BPH progression over time.Therefore,the inflammatory response involved in BPH may be a prime therapeutic target.

  11. Three-year outcome analysis of alpha 1-blocker naftopidil for patients with benign prostatic hyperplasia in a prospective multicenter study in Japan

    Science.gov (United States)

    Masumori, Naoya; Tsukamoto, Taiji; Shibuya, Akihiko; Miyao, Noriomi; Kunishima, Yasuharu; Iwasawa, Akihiko

    2016-01-01

    Purpose Our aim was to prospectively analyze the 3-year outcomes of naftopidil treatment for patients with benign prostatic hyperplasia (BPH), including those who dropped out during follow-up and had retreatment for BPH after termination of the drug within 3 years. Patients and methods Naftopidil, 50 mg/d or 75 mg/d, was given to 117 patients having BPH aged 50 years and older who had international prostate symptom scores (IPSS) ≥8. They were prospectively followed for 3 years with periodic evaluation. If naftopidil was terminated, the reason was determined. For patients with termination, an outcome survey was done to evaluate the status of retreatment for BPH at 3 years. Results Twenty-five patients (21.4%) continued the same medication for 3 years. The total IPSS, quality of life index, BPH problem index, and maximum flow rate were significantly improved during 3 years. Treatment failure defined as symptomatic progression (an increase in the IPSS of ≥4 points compared to the baseline value), development of acute urinary retention, conversion to other α1-blockers, add-on of a 5α-reductase inhibitor, or conversion to surgery was observed in 41 patients (35.0%). In the univariate analysis, age, prostate volume, and serum prostate-specific antigen were predictors of treatment failure. Of the 50 patients who discontinued naftopidil during the follow-up, only 13 (26%) patients reported that they needed retreatment with α1-blockers and/or surgery within 3 years. Conclusion Long-term efficacy of naftopidil was observed, although older age, increased prostate volume, and elevated prostate-specific antigen at baseline were highly likely to result in treatment failure. Even after termination for various reasons, only a small portion of the patients needed retreatment for BPH within 3 years. PMID:27524886

  12. Matrix metalloproteinase 9 expression in primary human prostatic adenocarcinoma and benign prostatic hyperplasia.

    Science.gov (United States)

    Hamdy, F. C.; Fadlon, E. J.; Cottam, D.; Lawry, J.; Thurrell, W.; Silcocks, P. B.; Anderson, J. B.; Williams, J. L.; Rees, R. C.

    1994-01-01

    Matrix metalloproteinase (MMP) expression was investigated in patients with prostatic adenocarcinoma and benign prostatic hyperplasia (BPH). Forty-one men were studied: 26 had histologically proven prostate cancer, with 14 (54%) showing metastatic disease; 15 patients had BPH. Prostatic tissue was obtained from transurethral resection and needle core biopsies; gelatinolytic activity was determined by zymography. Seven gelatinolytic bands were detected, with molecular weights ranging from > 100 kilodalton (kDa) to 29 kDa. Nine of 14 patients (64%) with skeletal metastases had 92 kDa activity, present in only two of 12 patients (17%) with a negative bone scan, and absent in BPH. The 92 kDa gelatinolytic activity was expressed in 73% of aneuploid tumours compared with 20% of diploid tumours. A 97 kDa gelatinase was expressed in 80% of BPH samples and 23% of carcinoma patients. Enzyme bands of 72, 66 and 45 kDa were equally expressed in malignant tissue, irrespective of metastatic status, but were expressed in fewer BPH patients. The 97, 92, 66 and 45 kDa enzymes were identified as being pro-MMP-9 sequences by Western blotting, using a specific antibody directed against the pro sequence of the mature protein. MMP activity appeared to be increased in malignant prostatic tissue compared with BPH. Pro-MMP-9, in its 92 kDa form, was shown to be exclusively expressed by malignant prostatic tissue, and in particular by tumours that exhibited the aggressive and metastatic phenotype. Images Figure 1 Figure 2 PMID:7506923

  13. Dutasteride: an evidence-based review of its clinical impact in the treatment of benign prostatic hyperplasia

    Directory of Open Access Journals (Sweden)

    Andrew Thomson

    2005-06-01

    Full Text Available Andrew ThomsonCore Medical Publishing, Knutsford, UKIntroduction: Benign prostatic hyperplasia (BPH is a common condition affecting older men. Bothersome symptoms can progress to serious complications such as acute urinary retention (AUR requiring surgical intervention. Dutasteride, a dual 5-alfa-reductase (5AR inhibitor (5ARI, is a recently introduced therapy for the treatment of BPH. Aims: The objective of this article is to review the evidence for the treatment of BPH with dutasteride. Evidence review: Evidence from large clinical studies shows that men with an enlarged prostate achieve a measurable decrease in prostate volume by up to 26% after 4 years of treatment with dutasteride and urinary symptoms improve after 6 months of treatment. This is achieved by rapid suppression (through inhibition of 5AR of the principal androgen (dihydrotestosterone or DHT responsible for stimulating prostatic growth. Evidence suggests that dutasteride treatment results in a reduction in risk (rather than delay of the most serious complications including episodes of AUR and the need for BPH-related surgery. Early symptom relief has been achieved with the combination of an alfa blocker and dutasteride. There is good evidence that dutasteride is well tolerated; side effects limited to sexual dysfunction (reduced libido, impotence, and gynecomastia are more common compared with placebo but occur with a similar incidence to finasteride, another 5ARI. No pharmacoeconomic evidence from studies with dutasteride has so far been published.Clinical value: In conclusion, dutasteride is a valuable treatment option in men with moderate to severe BPH. Reductions in prostate volume lead to symptom relief and serious complications appear to be reduced.Key words: dutasteride, evidence-based review, benign prostatic hyperplasia (BPH, 5-alfa-reductase inhibitor

  14. Down-regulated CFTR During Aging Contributes to Benign Prostatic Hyperplasia.

    Science.gov (United States)

    Xie, Chen; Sun, Xiao; Chen, Jing; Ng, Chi Fai; Lau, Kin Mang; Cai, Zhiming; Jiang, Xiaohua; Chan, Hsiao Chang

    2015-08-01

    Benign prostatic hyperplasia (BPH) is a hyper-proliferative disease of the aging prostate; however, the exact mechanism underlying the development of BPH remains incompletely understood. The present study investigated the possible involvement of the cystic fibrosis transmembrane conductance regulator (CFTR), which has been previously shown to negatively regulate nuclear factor-κB (NF-κB)/cyclooxygenase 2 (COX2)/prostaglandin E2 (PGE2) pathway, in the pathogenesis of BPH. Our results showed decreasing CFTR and increasing COX2 expression in rat prostate tissues with aging. Furthermore, suppression of CFTR led to increased expression of COX2 and over-production of PGE2 in a normal human prostate epithelial cell line (PNT1A) with elevated NF-κB activity. PGE2 stimulated the proliferation of primary rat prostate stromal cells but not epithelial cells, with increased PCNA expression. In addition, the condition medium from PNT1A cells after inhibition or knockdown of CFTR promoted cell proliferation of prostate stromal cells which could be reversed by COX2 or NF-κB inhibitor. More importantly, the involvement of CFTR in BPH was further demonstrated by the down-regulation of CFTR and up-regulation of COX2/NF-κB in human BPH samples. The present results suggest that CFTR may be involved in regulating PGE2 production through its negative regulation on NF-κB/COX2 pathway in prostate epithelial cells, which consequently stimulates cell growth of prostate stromal cells. The overstimulation of prostate stromal cell proliferation by down-regulation of CFTR-enhanced PGE2 production and release during aging may contribute to the development of BPH.

  15. GNA Confers Resistance to Rice Brown Planthopper in Transgenic Rice Pure Lines by Both Bioassay and Feeding Tests%生物鉴定和喂养试验证明GNA赋予转基因水稻纯系对褐飞虱的抗性

    Institute of Scientific and Technical Information of China (English)

    唐克轩; 孙小芬; 万丙良; 戚华雄; 卢兴桂

    2001-01-01

    Two transgenic rice pure lines, generated by particle bombardment, were challeng ed by the first-instar nymphs of rice brown planthopper (Nilaparvata lugens, BPH) biotype I using the standard BPH bioassay and feeding test protocol essen tially the same as described by Rao et al. (1998). Both lines contained and expressed the selectable marker hygromycin phosphotransferase gene(hpt), the reporter β-glucuronidase gene(gusA) and the snowdrop (Galanthus niv alis) lectin gene (gna). Insect bioassay and feeding test results showed t hat both lines had significant deterrent effect on BPH by decreasing BPH surviva l and overall fecundity, retarding BPH development and declining BPH feeding.%参照Rao等(1998)的褐飞虱生物鉴定和喂养方法,用水稻褐飞虱生物Ⅰ型的一龄若虫食喂, 用基因枪法获得2个转基因水稻纯系. 这2个纯系均含有并表达潮霉素抗性基因(hpt)、 gusA报告基因和雪花莲凝集素基因(gna). 褐飞虱生物鉴定和喂养试验表明,水稻 纯系对褐飞虱具有显著的抑制作用. 具体表现为降低褐飞虱成活率和繁殖力、延缓褐飞虱发 育以及减少褐飞虱进食量. 通过褐飞虱生物鉴定和喂养试验证明,表达GNA的转基因水稻纯系对严重危害水稻生产的褐飞虱具有抗性作用.

  16. Lower urinary tract symptoms and benign prostatic hyperplasia and their impact on quality of life

    Directory of Open Access Journals (Sweden)

    Antonio Carbone

    2015-11-01

    Full Text Available LUTS is an acronym that refers to symptoms affecting the lower urinary tract, which are very common in elderly subjects (between 60 and 70%, and often associated with, but not always caused by, benign prostatic hypertrophy (BPH. BPH is a chronic condition characterized by an increase in the number of cells, particularly in the transition area of the prostate. BPH involves a compression of the surrounding tissues, consequently obstructing vescical voiding. Nycturia and urgency represent the most prevalent symptoms and those with the greatest impact on quality of life measured as urinary-specific health-related quality. The prevalence of BPH is directly proportional to age; therefore, the absolute number of subjects affected is growing throughout the world. BPH is one of the most common medical conditions affecting those over 50. The overall cost for the diagnosis and treatment of BPH-related LUTS, in the US, has been estimated at approximately 1.1 billion US$/year, compared to total annual expenditure for urological conditions of some 9 billion and this cost continues to increase. The quick prostate test, which was developed by the Italian Urology Society (SIU, is an easy to use instrument that can be utilized in first-level screening for evaluation of the male population with LUTS. This test can be used both in patients not on pharmacological treatment and as a therapy-monitoring instrument. A positive response to one of the questions is sufficient for requesting a more in-depth investigation, which may provide indications on the therapeutic strategy to be taken.

  17. The treatment effects of flaxseed-derived secoisolariciresinol diglycoside and its metabolite enterolactone on benign prostatic hyperplasia involve the G protein-coupled estrogen receptor 1.

    Science.gov (United States)

    Ren, Guan-Yu; Chen, Chun-Yang; Chen, Wei-Guo; Huang, Ya; Qin, Li-Qiang; Chen, Li-Hua

    2016-12-01

    Secoisolariciresinol diglucoside (SDG), a lignan extracted from flaxseed, has been shown to suppress benign prostatic hyperplasia (BPH). However, little is known about the mechanistic basis for its anti-BPH activity. The present study showed that enterolactone (ENL), the mammalian metabolite of SDG, shared the similar binding site of G1 on a new type of membranous estrogen receptor, G-protein-coupled estrogen eceptor 1 (GPER), by docking simulations method. ENL and G1 (the specific agonist of GPER) inhibited the proliferation of human prostate stromal cell line WPMY-1 as shown by MTT assay and arrested cell cycle at the G0/G1 phase, which was displayed by propidium iodide staining following flow cytometer examination. Silencing GPER by short interfering RNA attenuated the inhibitory effect of ENL on WPMY-1 cells. The therapeutic potential of SDG in the treatment of BPH was confirmed in a testosterone propionate-induced BPH rat model. SDG significantly reduced the enlargement of the rat prostate and the number of papillary projections of prostatic alveolus and thickness of the pseudostratified epithelial and stromal cells when comparing with the model group. Mechanistic studies showed that SDG and ENL increased the expression of GPER both in vitro and in vivo. Furthermore, ENL-induced cell cycle arrest may be mediated by the activation of GPER/ERK pathway and subsequent upregulation of p53 and p21 and downregulation of cyclin D1. This work, in tandem with previous studies, will enhance our knowledge regarding the mechanism(s) of dietary phytochemicals on BPH prevention and ultimately expand the scope of adopting alternative approaches in BPH treatment.

  18. Comparing gene expression profiles between Bt and non-Bt rice in response to brown planthopper infestation

    Directory of Open Access Journals (Sweden)

    Fang eWang

    2015-12-01

    Full Text Available Bt proteins are the most widely used insecticidal proteins in transgenic crops for improving insect resistance. We previously observed longer nymphal developmental duration and lower fecundity in brown planthopper (BPH fed on Bt rice line KMD2, although Bt insecticidal protein Cry1Ab could rarely concentrate in this non-target rice pest. In the present study, we performed microarray analysis in an effort to detect Bt-independent variation, which might render Bt rice more defensive and/or less nutritious to BPH. We detected 3,834 and 3,273 differentially expressed probe-sets in response to BPH infestation in non-Bt parent Xiushui 11 and Bt rice KMD2, respectively, only 439 of which showed significant differences in expression between rice lines. Our analysis revealed a shift from growth to defense responses in response to BPH infestation, which was also detected in many other studies of plants suffering biotic and abiotic stresses. Chlorophyll biosynthesis and basic metabolism pathways were inhibited in response to infestation. IAA and GA levels decreased as a result of the repression of biosynthesis-related genes or the induction of inactivation-related genes. In accordance with these observations, a number of IAA-, GA-, BR-signaling genes were downregulated in response to BPH. Thus, the growth of rice plants under BPH attack was reduced and defense related hormone signaling like JA, SA and ET were activated. In addition, growth-related hormone signaling pathways, such as GA, BR and auxin signaling pathways, as well as ABA, were also found to be involved in BPH-induced defense. On the other side, 51 probe-sets (represented 50 genes that most likely contribute to the impact of Bt rice on BPH were identified, including three early nodulin genes, four lipid metabolic genes, 14 stress response genes, three TF genes and genes with other functions. Two transcription factor genes, bHLH and MYB, together with lipid transfer protein genes LTPL65 and

  19. AB013. The study on the diagnostic value of f/tPSA to the prostate cancer with tPSA greater than 10 ng/mL

    OpenAIRE

    Li, Yan-Feng; Wang, Qi

    2016-01-01

    Background To investigate the differential value of the percentage of free to total prostate specific antigen (f/tPSA) in the diagnosis of prostate cancer (PCa) and benign prostatic hyperplasia (BPH) when the tPSA greater than or equal to 10 ng/mL. Methods A retrospective study was performed among 361 cases of PCa or BPH whose tPSA greater than or equal to 10 ng/mL and whose diagnosis were confirmed by pathology through prostate biopsy or prostate surgery in our hospital from December 1, 2011...

  20. [Transurethral radiofrequency thermal destruction as the method of choice in patients with benign prostatic hyperplasia with a high degree of surgical risk and the presence of a cystostoma (or pronounced infravesical obstruction with preserved micturition)].

    Science.gov (United States)

    Apolikhin, O I; Sivkov, A V; Dorofeev, S D; Oshchepkov, V N; Tolstova, S S

    1997-01-01

    The authors employed transurethral radiofrequency thermodestruction (TRT) in 27 BPH patients with infravesical obstruction and grave somatic status excluding radical surgery. 17 of them had cystostomic drainage. A single procedure was not long and ran without general anesthesia. In patients able to urinate objective and subjective improvement was recorded, in those with cystostomic drainage suprapubic fistula was eliminated in 10 patients. In some cases TRT produced the results comparable to transurethral resection of the prostate. TRT complications comprise mainly infection and inflammation (11.1%). By efficacy and safety TRT occupies an intermediate position between thermotherapy and prostatic transurethral resection. This method is alternative in BPH patients with cystostoma and severe associated diseases.

  1. Extravasation of Urine Associated with Bilateral Complete Ureteral Duplication, Vesicoureteral Reflux and Benign Prostatic Hyperplasia.

    Science.gov (United States)

    Suzuki, Issei; Kaga, Kanya; Takei, Kohei; Tokura, Yuumi; Sakamoto, Kazumasa; Nishihara, Daisaku; Mizuno, Tomoya; Yuki, Hideo; Betsunoh, Hironori; Abe, Hideyuki; Yashi, Masahiro; Fukabori, Yoshitatsu; Yamanishi, Tomonori; Kamai, Takao

    2017-02-01

    We report a rare case of extravasation of urine, which may be associated with bilateral complete ureteral duplication, vesicoureteral reflux (VUR), and benign prostatic hyperplasia (BPH). A 71-year-old male presented with a complaint of right abdominal pain. An extravasation of urine was noted, and was improved by indwelling urethral catheterization. Transurethral resection of the prostate and the endoscopic subureteral injection of dextanomer/hyaluronic acid were performed for the treatment of BPH and VUR, respectively. The post-surgery recovery was successful.

  2. Experience with the combination of dutasteride and tamsulosin in the long-term management of benign prostatic hyperplasia.

    Science.gov (United States)

    Chughtai, Bilal; Elterman, Dean S; Lee, Richard; Te, Alexis E; Kaplan, Steven A

    2012-10-01

    Lower urinary tract symptoms (LUTS) caused by benign prostatic hyperplasia (BPH) commonly affect older men. These bothersome symptoms can lead to a decreased quality of life. Currently, two classes of drugs - α-adrenergic blockers and 5α-reductase inhibitors - are prescribed to treat LUTS secondary to BPH. Due to their different mechanisms of action, these medications work in a synergistic manner. Trials of combination therapy have been conducted to assess its effect compared with monotherapy. Current data support combination therapy in men with moderately enlarged prostates and moderate to severe symptoms.

  3. Effects of acoustic treatment on the interior noise levels of a twin-engine propeller aircraft - Experimental flight results and theoretical predictions

    Science.gov (United States)

    Beyer, T. B.; Powell, C. A.; Daniels, E. F.; Pope, L. D.

    1984-01-01

    In-flight noise level measurements were made within two cabin configurations of a general aviation business aircraft. The Fairchild Merlin IVC twin-engine aircraft was tested with bare walls and fiberglass insulation and in an executive trim configuration. Narrow-band and octave format data were subjected to analyses which permitted identification of the blade passage harmonics (BPH). Cabin noise level reductions (insertion losses) due to added insulation varied with position in the cabin, the BPH number, cabin pressure, and engine torque. The measurements were closely predicted using the propeller aircraft interior noise (PAIN) mode.

  4. The expression and clinical significance of BRAF in prostate cancer%BRAF在前列腺癌组织中表达及意义

    Institute of Scientific and Technical Information of China (English)

    Zhihong Wen; Xiaoli Zou; Guang Yang; Hui Wang

    2011-01-01

    Objective:The aim of this study was to investigate the expression of BRAF of prostate cancer,and to explore its relations with clinic-pathological factors.Methods:Immunohistochemistry was used to detect the expression of BRAF in 74 cases of prostate cancer,and 51 cases of benign prostate hyperplasia (BPH).Results:The positive rate of BRAF had significant difference between patients with prostate cancer and BPH (P 0.05).Conclusion:The expression of BRAF may play a role in the tumorigenesis and progression of colorectal cancer.BRAF could be a factor to diagnose the typing of prostate cancers and predict the prognosis of prostate cancers.

  5. Effects of endosymbiote on feeding, development, and reproduction of brown planthopper, Nilaparvata lugens stal

    Institute of Scientific and Technical Information of China (English)

    2001-01-01

    @@The biotype 1,2, and 3 of N. Lugensobtained from IRRI, the Philippines, were reared continuously on susceptible variety TN1, resistant varieties Mudgo (Bph1), and ASD7 (Bph2),respectively. To eliminate the yeast-like symbionts (YLS) in N.lugens,about 60-d old plants of TN1 bearing 3-d old eggs were laid at 26℃ for 2 d and exposed at 35℃ in an incubator for 3 d. After a hot treatment, the plants were transferred into an incubator at 26℃ for egg development. The hatched aposymbiotic nymphs were employed in this experiment.

  6. Изучение фармакологической эффективности густых экстрактов ярутки полевой и эспарцета песчаного на модели доброкачественной гиперплазии предстательной железы у крыс

    OpenAIRE

    2015-01-01

    The benign prostatic hyperplasia (BPH) is the most wide spread urologic pathology of men of "gold" reproductive age, and preventive care and treatment of BPH is an issue of acute importance for urologists and andrologists of the whole world. Research of the last decade let to say about obvious tendency of this disease progression, that is caused by rise of a menhood rate of the elder age groups. The range of medicines for the treatment of prostatic diseases, presented at the world market, is ...

  7. Arm-in-Arm Response Regulator Dimers Promote Intermolecular Signal Transduction

    Science.gov (United States)

    Baker, Anna W.; Satyshur, Kenneth A.; Moreno Morales, Neydis

    2016-01-01

    ABSTRACT Bacteriophytochrome photoreceptors (BphPs) and their cognate response regulators make up two-component signal transduction systems which direct bacteria to mount phenotypic responses to changes in environmental light quality. Most of these systems utilize single-domain response regulators to transduce signals through unknown pathways and mechanisms. Here we describe the photocycle and autophosphorylation kinetics of RtBphP1, a red light-regulated histidine kinase from the desert bacterium Ramlibacter tataouinensis. RtBphP1 undergoes red to far-red photoconversion with rapid thermal reversion to the dark state. RtBphP1 is autophosphorylated in the dark; this activity is inhibited under red light. The RtBphP1 cognate response regulator, the R. tataouinensis bacteriophytochrome response regulator (RtBRR), and a homolog, AtBRR from Agrobacterium tumefaciens, crystallize unexpectedly as arm-in-arm dimers, reliant on a conserved hydrophobic motif, hFWAhL (where h is a hydrophobic M, V, L, or I residue). RtBRR and AtBRR dimerize distinctly from four structurally characterized phytochrome response regulators found in photosynthetic organisms and from all other receiver domain homodimers in the Protein Data Bank. A unique cacodylate-zinc-histidine tag metal organic framework yielded single-wavelength anomalous diffraction phases and may be of general interest. Examination of the effect of the BRR stoichiometry on signal transduction showed that phosphorylated RtBRR is accumulated more efficiently than the engineered monomeric RtBRR (RtBRRmon) in phosphotransfer reactions. Thus, we conclude that arm-in-arm dimers are a relevant signaling intermediate in this class of two-component regulatory systems. IMPORTANCE BphP histidine kinases and their cognate response regulators comprise widespread red light-sensing two-component systems. Much work on BphPs has focused on structural understanding of light sensing and on enhancing the natural infrared fluorescence of these

  8. Modeling the contributions of phytoplankton and non-algal particles to spectral scattering properties in near-shore and lagoon waters

    Science.gov (United States)

    Vadakke-Chanat, Sayoob; Shanmugam, Palanisamy

    2017-03-01

    Particular attention was focused on modeling the spectral scattering properties of phytoplankton (bph(λ)) and non-algal particles (detrital organic and inorganic sediments bNAP(λ)) from absorption and attenuation measurements in near-shore and lagoon waters. The absorption line height (aLH(676)) measured above a linear background between 648 nm and 714 nm in particulate and dissolved organic matter absorption spectra (ap(λ)) is a spectral feature that is primarily associated with the chlorophyll with significantly less pigment package effect compared to the blue peak, and hence it is solely attributed to the phytoplankton absorption (aph). The correlation of aph(λ) with bph(λ) in terms of the spectral shape and the relation of aLH(676) with chlorophyll concentration hold the key to derive bph(648) from the aLH(676) measurements. bNAP(648) values are then determined by subtracting the bph(648) from bp(648), allowing the power-law model to derive the bNAP(λ). In-situ determination of bph (λ) is subsequently achieved by subtracting the featureless bNAP(λ) from bp(λ) provided by the ac-s sensor. These data form the basis for the development of models for independent estimates of bph(λ) and bNAP(λ) based on the measurements of aLH and suspended sediment concentration or turbidity. The validity of this method was demonstrated in a wide variety of samples from coastal and inland environments. Comparison of the modeled and measured spectral variations of bph(λ) showed the mean relative percent difference between these two data to be within 20%. bNAP(λ) predictions also had an error a few percent and the correlation coefficient close to unity. When comparing the modeled bph(λ) with laboratory culture data, the results were exceptionally good although discrepancies in size and refractive index of cells of monospecific lab culture samples and natural assemblages due to the simultaneous presence of different species. The proposed approach and models are highly

  9. APPLICATION OF TRANSURETHRAL MICROWAVE THERMOTHERAPY FOR PATIENTS WITH ACUTE URINARY RETENTION AND SEVERE COMBINED COMORBIDITY FROM BENIGN PROSTATIC HYPERPLASIA

    Directory of Open Access Journals (Sweden)

    A. Mysak

    2014-12-01

    Conclusions. Taking into consideration the minimally invasive nature, favourable tolerability and absence of adverse effects, TUMT can be considered a method of choice in patients with BPH-triggered AUR and contraindications to major surgical treatments and general anaesthesia. Remote outcomes of TUMT may be evaluated as satisfactory, with good effects in 71.62% patients. However in prostatic volumes exceeding 85 cm3 and pronounced intravesical pattern of BPH growth the efficcacy of TUMT is arguable. KEY WORDS: benign prostatic hyperplasia, acute urinary retention, transurethral microwave hermotherapy.

  10. [THE PATIENTS QUALITY OF LIFE ESTlMATION IN PRESENCE OF METASTASIS OF A RENAL-CELL CANCER IN THE BONES ON BACKGROUND OF THE BISPHOSPHONATES APPLICATION].

    Science.gov (United States)

    Boychuk, S I; Dedkov, A G; Volkov, I B; Kovahlichuk, P A; Kostyuk, V Yu

    2016-04-01

    Results of the patients quality of life (QL) estimation, while presence of a renal-cell cancer metastasis in the bones, using questionnaire QLQ-C30 and Karnofsky index, as well as the pain visual-analogue scale on background of treatment with bisphosphonates (BPH), were adduced. After conclusion of the combined treatment the general state improvement, a trustworthy reduction of the pain syndrome severity, as well as the patients' psychoemotional and social state, were noted. Complex treatment of patients, using BPH, have promoted a positive impact on their QL as well as a reduction of a skeleton complications rate.

  11. Individual extraction constants of some univalent anions in the two-phase water-phenyltrifluoromethyl sulfone system.

    Science.gov (United States)

    Makrlík, Emanuel; Selucký, Pavel; Vaňura, Petr

    2011-12-01

    From extraction experiments and g-activity measurements, the extraction constants corresponding to the general equilibrium Cs+(aq) + A- (aq) Cs+(org) + A- (org) taking place in the two-phase water-phenyltrifluoromethyl sulfone (FS 13) system (A-= I-, ClO4-, MnO4-, Br-3, I-3, picrate, tetraphenylborate (BPh-4); aq = aqueous phase, org = FS 13 phase) were evaluated. Furthermore, the individual extraction constants of these 7 anions in the mentioned two-phase system were calculated; they were found to increase in the series of I-< ClO4- < Br-3 < MnO4-, picrate < I-3 < BPh-4.

  12. Effects of Kangquan Recipe(康泉方) on Sex Steroids and Cell Proliferation in Rats with Benign Prostatic Hyperplasia

    Institute of Scientific and Technical Information of China (English)

    黄源鹏; 杜建; 洪振丰; 陈治卿; 吴锦发; 赵锦燕

    2009-01-01

    Objective:To investigate the effects of Kangquan Recipe(康泉方,KQR)on sex steroids and cell proliferation in an experimental benign prostatic hyperplasia(BPH)model in rats.Methods:Seventy-two SD rats were randomly divided into six groups:the normal group,the model group,the finasteride group,and the low-, middle-,and high-dose KQR groups,12 in each group.Except those in the normal group,the rats were injected with testosterone after castration for the establishment of BPH model and then given respectively w...

  13. Differential expression of 5-alpha reductase isozymes in the prostate and its clinical implications

    Directory of Open Access Journals (Sweden)

    Kai Wang

    2014-04-01

    Full Text Available The development of human benign or malignant prostatic diseases is closely associated with androgens, primarily testosterone (T and dihydrotestosterone (DHT. T is converted to DHT by 5-alpha reductase (5-AR isozymes. Differential expression of 5-AR isozymes is observed in both human benign and malignant prostatic tissues. 5-AR inhibitors (5-ARI are commonly used for the treatment of benign prostatic hyperplasia (BPH and were once promoted as chemopreventive agents for prostate cancer (PCa. This review discusses the role of the differential expression of 5-AR in the normal development of the human prostate and in the pathogenesis and progression of BPH and PCa.

  14. Study of microorganisms degrading PCB in vegetated contaminated soil

    Directory of Open Access Journals (Sweden)

    Veronika Kurzawova

    2010-12-01

    Full Text Available Removal of PCBs from contaminated soil is one of the challenges ofenvironmental microbiology. In our study, we aimed to isolate,characterize and identify microorganisms from contaminated soiland to find out the plant effect on microbial diversity in theenvironment. Microorganisms were isolated by two ways, directextraction and isolation after cultivation with biphenyl as a solesource of carbon. Isolated bacteria were biochemically characterizedand the composition of ribosomal proteins in bacterial cells wasdetermined by mass spectrometry MALDI-TOF. Bacteria withrequired properties were chosen and the bphA gene was amplifiedand detected. Bacteria with detected bphA gene were then identifiedby 16S rRNA sequence analyses.

  15. 经尿道前列腺电切术89例治疗体会

    Institute of Scientific and Technical Information of China (English)

    黄伟; 娄斌; 刘磊

    2011-01-01

    @@ 良性前列腺增生(Benign Hypertrophy of Prostate,BPH)是老年男性的常见病.经尿道前列腺电切术(Transurethral Electroresection of Prostate,TURP)具有手术时间短、并发症少、住院时间短、疗效好等特点,被公认为是治疗良性前列腺增生的“金标准”.我院2006年1月-2010年10月共实施TURP治疗BPH 89例,效果满意,报告如下.

  16. Immunohistochemical Studies of the Expression of Matrix Metalloproteinase-2 and Metalloproteinase-9 in Human Prostate Cancer

    Institute of Scientific and Technical Information of China (English)

    曾汉青; 肖亚军; 鲁功成; 陈勇

    2003-01-01

    To study the expression of matrix metalloproteinase-2 and -9 in human prostate cancer,matrix metalloproteinase-2 and -9 were immunohistochemically detected in tissues of prostate cancer and benign prostatic hyperplasia (BPH). Our results showed that matrix metalloproteinase-2 and -9 levels in prostate cancer were much higher than those in tissues of BPH, with the cancer invasion being positively correlated with the expression of the metalloproteinases. It is concluded that matrix metalloproteinase-2 and -9 are better molecular markers, which are of help in the diagnosis and prediction of prognosis of prostate cancer.

  17. Clinical analysis of binign prostatic hyperplasia complicated with chronic prostatitis in 52 cases%良性前列腺增生合并慢性前列腺炎患者的临床特点分析

    Institute of Scientific and Technical Information of China (English)

    黄固山; 李云最; 张华旦; 韦辉柳; 莫恩平

    2016-01-01

    Objective To explore the clinical features of binign prostatic hyperplasia ( BPH) complicated with chronic prostatitis ( CP ) .Methods Ninety-six patients who underwent transurethrue resection of prostate ( TURP) for the treatment of binign prostatic hyperplasia were collected in our hospital from January 2010 to Novem-ber 2014.They were divided into BPH with CP group(n=52) and BPH group(n=44) according to the pathological diagnosis after the operation.The International Prostate Symptom Score(IPSS), quality of life(QOL) score, maxi-mum urinary flow rate( Qmax) and residual urine volume were compared between the two groups.The relationship be-tween prostate volume and the incidence of BPH complicated with CP and the relationship between IPSS scores and the levels of CP were analyzed.Results The prostate volume, QOL and residual urine volume in the BPH with CP group were significantly higher than those in the BPH group(P<0.05), while the IPSS scores and Qmax in the BPH with CP group were signigicantly lower than those in the BPH group(P<0.05).There were significantly positive cor-relations between prostate volume and CP occurred and between IPSS scores and the levels of CP(P<0.05).Conclu-sion Compared with the patients with simple BPH, the patients with BPH complicated with CP have greater prostate volume and more serious lower urinary tract symptoms.CP plays an important role in the development of BPH.%目的:探讨良性前列腺增生( BPH)合并慢性前列腺炎( CP)患者的临床特点。方法选择2010-01~2014-11该院收治96例BPH患者,均行经尿道前列腺电切术( TURP)治疗,根据术后标本病理检查结果分为BPH合并CP组(52例)和单纯BPH组(44例),比较两组患者国际前列腺症状评分(IPSS)、生活质量评分(QOL)、最大尿流率(Qmax)、剩余尿量等,并分析前列腺体积与CP发生之间以及IPSS与CP程度分级之间的相关性。结果 BPH合并CP组前列腺体积

  18. 78 FR 23565 - Radio Broadcasting Services; AM or FM Proposals To Change the Community of License.

    Science.gov (United States)

    2013-04-19

    ... COMMISSION Radio Broadcasting Services; AM or FM Proposals To Change the Community of License. AGENCY... BROADCASTING, INC., Station KAKS, Facility ID 69858, BPH- 20130318AGJ, From HUNTSVILLE, AR, To GOSHEN, AR; CARROLL COUNTY BROADCASTING, INC., Station KTHS, Facility ID 35668, BP-20130318AGK, From BERRYVILLE,...

  19. 75 FR 1621 - Radio Broadcasting Services; AM or FM Proposals To Change the Community of License

    Science.gov (United States)

    2010-01-12

    ... COMMISSION Radio Broadcasting Services; AM or FM Proposals To Change the Community of License AGENCY: Federal... ELDON, MO, To ST. THOMAS, MO; COX RADIO, INC., Station WALR-FM, Facility ID 48728, BPH-20091124ABA, From... 8438, BMP-20091125ABD, From ASHLAND, VA, To POWHATAN, VA; HAMPTONS COMMUNITY RADIO CORPORATION,...

  20. 76 FR 55388 - Radio Broadcasting Services; AM or FM Proposals To Change the Community of License.

    Science.gov (United States)

    2011-09-07

    ... COMMISSION Radio Broadcasting Services; AM or FM Proposals To Change the Community of License. AGENCY... proposals to change the community of license: CBS Radio East Inc., Station WLZL, Facility ID 72177, BPH-20110812ACL, from Annapolis, MD, to Bowie, MD; Indiana Community Radio Corporation, Station WYER, Facility...

  1. 78 FR 69086 - Radio Broadcasting Services; AM or FM Proposals To Change the Community of License

    Science.gov (United States)

    2013-11-18

    ... COMMISSION Radio Broadcasting Services; AM or FM Proposals To Change the Community of License AGENCY: Federal... the community of license: CBS RADIO ANNAPOLIS LLC, Station WLZL, Facility ID 20983, BMPH-20131022ALD..., BPH-20130924AJT, From BATH, NY, To AVOCA, NY; PRITCHARD BROADCASTING CORPORATION, Station...

  2. 75 FR 74732 - Radio Broadcasting Services; AM or FM Proposals To Change The Community of License

    Science.gov (United States)

    2010-12-01

    ... COMMISSION Radio Broadcasting Services; AM or FM Proposals To Change The Community of License AGENCY: Federal..., Facility ID 46969, BPH-20091230AAY, from Titusville, FL, to Deltona, FL; Hawaii Public Radio, Inc., Station KIPM, Facility ID 172438, BMPED-20101019ACS, from Hana, HI, to Waikapu, HI; Huron Broadcasting,...

  3. 78 FR 41062 - Radio Broadcasting Services; AM or FM Proposals To Change the Community of License

    Science.gov (United States)

    2013-07-09

    ... COMMISSION Radio Broadcasting Services; AM or FM Proposals To Change the Community of License AGENCY: Federal...-20130509ABB, From SMITHS GROVE, KY, To HISEVILLE, KY; HOG RADIO, INC., Station KCYT, Facility ID 51098, BPH- 20130603AES, From OZARK, AR, To FAYETTEVILLE, AR; JOY BROADCASTING, INC., Station WXGN, Facility ID...

  4. 76 FR 50732 - Radio Broadcasting Services; AM or FM Proposals To Change the Community of License

    Science.gov (United States)

    2011-08-16

    ... COMMISSION Radio Broadcasting Services; AM or FM Proposals To Change the Community of License AGENCY: Federal... the community of license: ALELUYA BROADCASTING NETWORK, Station NEW, Facility ID 123270, BMPED..., Facility ID 170991, BPH-20110620AGF, From HAMILTON, TX, To HICO, TX; NORMIN BROADCASTING CO., Station...

  5. ALFUZOSIN IN THE TREATMENT OF BENIGN PROSTATIC HYPERPLASIA - EFFECTS ON SYMPTOM SCORES, URINARY FLOW-RATES AND RESIDUAL VOLUME - A MULTICENTER, DOUBLE-BLIND, PLACEBO-CONTROLLED TRIAL

    NARCIS (Netherlands)

    HANSEN, BJ; NORDLING, J; MENSINK, HJA; WALTER, S; MEYHOFF, HH; LEENARTS, JAF; OOSTEN, JK; VANSOEST, FF; DIJKMAN, GA; HOEKSTRA, JW; VANBAASBANK, NJW; BIJLEVELD, RT; BRAAM, PFCM; SCHLATMANN, TJM; FELDERHOF, J; KHOE, GSS; DIK, P; MENSINK, HJA; SKOV, J; CHRISTOFFERSEN, J; GEERDSEN, JP; HVIDT, [No Value; DAHL, C; LUKE, M; LENDORPH, A; JACOBSEN, B; BILDE, T; MORTENSEN, S; LARSEN, EH

    1994-01-01

    In order to assess the efficacy and safety of alfuzosin, a selective alpha-1 receptor antagonist, 205 patients with Benign Prostatic Hyperplasia (BPH) were randomly assigned in a double-blind, placebo-controlled manner, to receive either alfuzosin 2,5 mg TID or placebo TID during 12 weeks. After 12

  6. Engineered FGF-2 expression induces glandular epithelial hyperplasia in the murine prostatic dorsal lobe.

    NARCIS (Netherlands)

    Takahashi, N.; Takeuchi, T.; Nishimatsu, H.; Kamijo, T.; Tomita, K.; Schalken, J.A.; Teshima, S.; Kitamura, T.

    2004-01-01

    OBJECTIVE: It is known that androgens and stromal-epithelial interactions are required for the formation and growth of the prostate. FGF-2 is overexpressed in prostatic stromal cells in benign prostatic hypertrophy (BPH)/prostate cancer. This supports the paracrine/autocrine growth of prostatic epit

  7. [Serum metabolomics analysis on benign prostate hyperplasia in mice based on liquid chromatography-mass spectrometry].

    Science.gov (United States)

    Geng, Yue; Sun, Fengxia; Ma, Yu; Deng, Ligang; Lü, Jianyun; Li, Teng; Wang, Congcong

    2014-12-01

    Benign prostatic hyperplasia (BPH) increasingly becomes a common factor affecting the quality of life of aging men. Its pathogenesis has not yet been fully elucidated. Ultra-high pressure liquid chromatography-quadrupole time-of-flight mass spectrometry (UPLC-QTOF-MS) was employed to detect the changes of serum metabolites in normal mice, benign prostatic hyperplasia model mice and BPH model mice with finasteride intervention. The serum metabolite profiles of the three groups of mice were analyzed. Partial least squares-discriminant analysis (PLS-DA) was used for group differentiation and biomarker selection. The results showed good distinction among the three groups of mice serum metabolite spectra. Three potential biomarkers, 1-hexadecanoyl-SN-glycero-3-phosphocholine, 1-O-hexadecyl-2-O-acetyl-sn-glyceryl-3-phosphorylcholine and (Z)-13-docosenamide, were discovered and identified. They all indicated the occurrence of benign prostatic hypertrophy is closely related to the disorders of lipid metabolism. Coinpared with the control group, the contents of the first two substances were significantly increased in the serum of BPH model mice, and significantly decreased after intervened by finasteride. The contents of (Z)-13-docosenamide decreased significantly in the serum of model group, and increased after intervened by finasteride. Compared with the control group, the contents of three biomarkers in finasteride group did not recover completely and had significant differences. This study is conductive to open new avenues of diagnosis and medical treatment for BPH.

  8. Differential blood-based diagnosis between benign prostatic hyperplasia and prostate cancer: miRNA as source for biomarkers independent of PSA level, Gleason score, or TNM status.

    Science.gov (United States)

    Leidinger, Petra; Hart, Martin; Backes, Christina; Rheinheimer, Stefanie; Keck, Bastian; Wullich, Bernd; Keller, Andreas; Meese, Eckart

    2016-08-01

    Since the benefit of prostate-specific antigen (PSA) screening remains controversial, new non-invasive biomarkers for prostate carcinoma (PCa) are still required. There is evidence that microRNAs (miRNAs) in whole peripheral blood can separate patients with localized prostate cancer from healthy individuals. However, the potential of blood-based miRNAs for the differential diagnosis of PCa and benign prostatic hyperplasia (BPH) has not been tested. We compared the miRNome from blood of PCa and BPH patients and further investigated the influence of the tumor volume, tumor-node-metastasis (TNM) classification, Gleason score, pretreatment risk status, and the pretreatment PSA value on the miRNA pattern. By microarray approach, we identified seven miRNAs that were significantly deregulated in PCa patients compared to BPH patients. Using quantitative real time PCR (qRT-PCR), we confirmed downregulation of hsa-miR-221* (now hsa-miR-221-5p) and hsa-miR-708* (now hsa-miR-708-3p) in PCa compared to BPH. Clinical parameters like PSA level, Gleason score, or TNM status seem to have only limited impact on the overall abundance of miRNAs in patients' blood, suggesting a no influence of these factors on the expression of deregulated miRNAs.

  9. Analysis of risk factors for benign prostatic hyperplasia and metabolic syndrome in the elderly undergoing health examination

    Institute of Scientific and Technical Information of China (English)

    王蕾蕾

    2013-01-01

    Objective To investigate the prevalences of benign prostatic hyperplasia (BPH) and metabolic syndrome (MS) and to analyze the risk factors for benign prostatic hyperplasia and metabolic syndrome in the elderly.Methods A total of 490 elderly men undergoing health examination in our hospital from January 2010 to

  10. Development of a decision aid for the treatment of benign prostatic hyperplasia : A four stage method using a Delphi consensus study

    NARCIS (Netherlands)

    Lamers, Romy E D; Cuypers, Maarten; Garvelink, Mirjam M.; Bosch, J. L H Ruud; Kil, Paul J M

    2016-01-01

    Objective: To develop a web-based decision aid (DA) for the treatment of lower urinary tract symptoms due to benign prostatic hyperplasia (LUTS/BPH). Methods: From February-September 2014 we performed a four-stage development method: 1: Two-round Delphi consensus method among urologists, 2: Identify

  11. Prostate-specific extracellular vesicles as a novel biomarker in human prostate cancer

    Science.gov (United States)

    Park, Yong Hyun; Shin, Hyun Woo; Jung, Ae Ryang; Kwon, Oh Sung; Choi, Yeong-Jin; Park, Jaesung; Lee, Ji Youl

    2016-01-01

    Extracellular vesicles (EVs) may play an important role in cancer development and progression. We aimed to investigate the prognostic potential of prostate-specific EVs in prostate cancer (PCa) patients. Plasma and prostate tissue were collected from patients who underwent surgery for PCa (n = 82) or benign prostatic hyperplasia (BPH, n = 28). To analyze the quantity of EVs in prostate, we performed transmission electron microscopy (TEM), immuno-TEM with CD63 and prostate-specific membrane antigen (PSMA), and immunofluorescence staining. After EV isolation from plasma, CD63 and PSMA concentration was measured using ELISA kits. PSMA-positive areas in prostate differed in patients with BPH, and low-, intermediate-, and high-risk PCa (2.4, 8.2, 17.5, 26.5%, p BPH, and low-, intermediate-, and high-risk PCa (21.9, 43.4, 49.2, 59.9 ng/mL, p BPH (AUC 0.943). Patients with lower plasma PSMA-positive EV concentration had greater prostate volume (50.2 vs. 33.4 cc, p pathologic Gleason score (p = 0.025). During the median follow-up of 18 months, patients with lower plasma PSMA-positive EV concentration tended to have a lower risk of biochemical failure than those with higher levels of prostate-specific EVs (p = 0.085). PMID:27503267

  12. Variation of prostate-specific antigen expression in different tumour growth patterns present in prostatectomy specimens

    NARCIS (Netherlands)

    M.P.W. Gallee; E. Visser-de Jong (E.); J.A.G.M. van der Korput (J. A G M); Th.H. van der Kwast (Theo); F.J.W. ten Kate (Fiebo); F.H. Schröder (Fritz); J. Trapman (Jan)

    1990-01-01

    textabstractA series of 55 randomly chosen radical prostatectomy specimens was analyzed for expression of prostate-specific antigen (PSA) by immunohistochemical techniques. Tissue sections were selected in such a manner that in addition to glandular benign prostatic hyperplasia (BPH), one or more di

  13. Metabolic Stress Induced by Arginine Deprivation Induces Autophagy Cell Death in Prostate Cancer

    Science.gov (United States)

    2011-08-01

    Cell biology, physiology and pathology . Oct 2009 4. Changou A, F Chuang, R Bold, HJ Kung. Use of a deconvoluting microscope to evaluate autophagy...mRNA from primary prostate tissue was examined for ASS expression by RT-PCR. D, normal liver as a positive control for cytoplasmic ASS staining. BPH

  14. Absolute Quantitation of DNA Methylation of 28 Candidate Genes in Prostate Cancer Using Pyrosequencing

    Directory of Open Access Journals (Sweden)

    Nataڑa Vasiljeviš

    2011-01-01

    Full Text Available Aberrant DNA methylation plays a pivotal role in carcinogenesis and its mapping is likely to provide biomarkers for improved diagnostic and risk assessment in prostate cancer (PCa. We quantified and compared absolute methylation levels among 28 candidate genes in 48 PCa and 29 benign prostate hyperplasia (BPH samples using the pyrosequencing (PSQ method to identify genes with diagnostic and prognostic potential.

  15. Oxidative Stress Indicators in Patients with Prostate Disorders in Enugu, South-East Nigeria

    Directory of Open Access Journals (Sweden)

    Romanda Duru

    2014-01-01

    Full Text Available Depletion of cellular antioxidants can result from free radical formation due to normal endogenous reactions and the ingestion of exogenous substances and environmental factors. The levels of reactive oxygen species-(ROS- scavenging enzymes such as SOD and glutathione peroxidase have been shown to be significantly altered in malignant cells and in primary cancer tissues. The aim of this study was to determine the antioxidant status of patients with prostate disorders in South-East Nigeria to ascertain the possible role of depletion of antioxidants in prostatic degeneration. 104 subjects made up of 40 PCa patients, 32 with BPH, and 32 controls participated in this study. The levels of superoxide dismutase, glutathione peroxidase, vitamin C, and vitamin E were estimated using standard procedures. The results show that both the BPH and PCa patients had a significant decrease (P<0.05 in GPX, SOD, vitamin C, and vitamin E levels compared to the control subjects. However, there was also a significant decrease (P<0.05 in SOD and vitamin C levels in PCa patients when compared with the BPH group. This indicates that patients with BPH and prostate cancer have decreased antioxidant status and may benefit from micronutrient supplementation.

  16. Pairwise Gibbs energies of interaction involving N-alkyl-2- pyrrolidinones and related compounds in aqueous solution obtained from kinetic medium effects

    NARCIS (Netherlands)

    Apperloo, Joke J.; Streefland, Lisette; Engberts, Jan B.F.N.; Blandamer, Michael J.

    2000-01-01

    Kinetic solvent effects of N-alkyl-2-pyrrolidinones and structurally related compounds on the water-catalyzed hydrolysis reactions of p-methoxyphenyl dichloroacetate (MPDA), 1-benzoyl-3-phenyl-1,2,4-triazole (BPhT), and 1-benzoyl-1,2,4-triazole (BT) in highly dilute aqueous solutions at pH 4 and 298

  17. Diagnostic and predictive value of voiding diary data versus prostate volume, maximal free urinary flow rate, and Abrams-Griffiths number in men with lower urinary tract symptoms suggestive of benign prostatic hyperplasia

    NARCIS (Netherlands)

    van Venrooij, Ger E. P. M.; van Melick, Harm H. E.; Eckhardt, Mardy D.; Boon, Tom A.

    2008-01-01

    OBJECTIVES To investigate the information of voiding data in relation to symptoms and well-being in men with lower urinary tract symptoms (LUTS) suggestive of benign prostatic hyperplasia (BPH) and to compare this information with that of prostate volume (Vprostate), maximal free urinary flow rate (

  18. Urodynamic implications of benign prostatic hyperplasia

    DEFF Research Database (Denmark)

    Jensen, K M; Andersen, J T

    1990-01-01

    By the age of 60, about 70% of men have developed benign prostatic hyperplasia (BPH), and 85%-95% of these have symptomatic dysfunction of the lower urinary tract, 10%-20% undergoing prostatectomy. Although transurethral resection of the prostate is generally considered to be a safe and effective...

  19. Biomarkers of Prostatic Cancer: An Attempt to Categorize Patients into Prostatic Carcinoma, Benign Prostatic Hyperplasia, or Prostatitis Based on Serum Prostate Specific Antigen, Prostatic Acid Phosphatase, Calcium, and Phosphorus.

    Science.gov (United States)

    Sarwar, Shahana; Adil, Mohammed Abdul Majid; Nyamath, Parveen; Ishaq, Mohammed

    2017-01-01

    Prostatitis, BPH, and P.Ca are the most frequent pathologies of the prostate gland that are responsible for morbidity in men. Raised levels of PSA are seen in different pathological conditions involving the prostate. PAP levels are altered in inflammatory or infectious or abnormal growth of the prostate tissue. Serum calcium and phosphorus levels were also found to be altered in prostate cancer and BPH. The present study was carried out to study the levels of PSA, PAP, calcium, and phosphorus in serum of patients with Prostatitis, BPH, or P.Ca and also to evaluate the relationship between them. Males in the age group of 50-85 years with LUTS disease symptoms and with PSA levels more than 4 ng/mL were included. A total of 114 patients were analyzed including 30 controls. Prostatitis in 35.7% of cases, BPH in 35.7% of the cases, and P.Ca in 28.57% of the cases were observed. Thus, the nonmalignant cases constitute a majority. PSA, a marker specific for prostatic conditions, was significantly high in all the diseases compared to controls. A rise in serum PSA and PAP indicates prostatitis or, in combination with these two tests, decreased serum calcium shows advanced disease.

  20. Ejaculatory dysfunction in the treatment of lower urinary tract symptoms.

    Science.gov (United States)

    DeLay, Kenneth Jackson; Nutt, Max; McVary, Kevin T

    2016-08-01

    The link between lower urinary tract symptoms (LUTS) secondary to benign prostatic hyperplasia (BPH) and sexual dysfunction is well established. Sexual dysfunction can encompass both ejaculatory dysfunction (EjD) and erectile dysfunction (ED). Ejaculatory dysfunction can consist of premature ejaculation, delayed ejaculation, retrograde ejaculation, anejaculation, decreased force of ejaculation and pain upon ejaculation. The impact of different medical and surgical therapies on ejaculatory function will be reviewed. We reviewed the various categories of LUTS treatment including the canonical epidemiology and pathophysiology as well as the surgical and medical treatments for LUTS/BPH. We note that most surgeries and several medical treatments have a certain but ill-defined negative impact on ejaculatory function. Several MISTs and selected medical therapies appear to have little impact on EjD. Both EjD and BPH are very common disorders in men under the care of an urologist. It is well documented that there is a clinical association between these two entities. Unfortunately many of the medical treatments and almost all surgical treatment impact the ejaculatory function of the patient. The surgical treatment of BPH often leads to retrograde ejaculation while medical treatment leads to anejaculation.

  1. Suppressing male spermatogenesis-associated protein 5-like gene expression reduces vitellogenin gene expression and fecundity in Nilaparvata lugens Stål

    Science.gov (United States)

    In our previous study with the brown planthopper (BPH), Nilaparvata lugens, triazophos (tzp, organophosphate) treatments led to substantial up-regulation of a male spermatogenesis-associated protein 5-like gene (NlSPATA5) compared to untreated controls. Mating with tzp-treated males significantly in...

  2. Review of naturopathy of medical mushroom, Ophiocordyceps sinensis, in sexual dysfunction

    Directory of Open Access Journals (Sweden)

    Kanitta Jiraungkoorskul

    2016-01-01

    Kanitta Jiraungkoorskul, received her B.PH. in Environmental Science and Technology, and M.Sc. in Industrial Hygiene and Safety with thesis in title “Factors affecting urinary cadmium level and health risk assessment among farmer in Phrathatphadaeng Subdistrict, Mae Sod District, Tak Province.

  3. Biomarkers of Prostatic Cancer: An Attempt to Categorize Patients into Prostatic Carcinoma, Benign Prostatic Hyperplasia, or Prostatitis Based on Serum Prostate Specific Antigen, Prostatic Acid Phosphatase, Calcium, and Phosphorus

    Science.gov (United States)

    Sarwar, Shahana; Nyamath, Parveen; Ishaq, Mohammed

    2017-01-01

    Prostatitis, BPH, and P.Ca are the most frequent pathologies of the prostate gland that are responsible for morbidity in men. Raised levels of PSA are seen in different pathological conditions involving the prostate. PAP levels are altered in inflammatory or infectious or abnormal growth of the prostate tissue. Serum calcium and phosphorus levels were also found to be altered in prostate cancer and BPH. The present study was carried out to study the levels of PSA, PAP, calcium, and phosphorus in serum of patients with Prostatitis, BPH, or P.Ca and also to evaluate the relationship between them. Males in the age group of 50–85 years with LUTS disease symptoms and with PSA levels more than 4 ng/mL were included. A total of 114 patients were analyzed including 30 controls. Prostatitis in 35.7% of cases, BPH in 35.7% of the cases, and P.Ca in 28.57% of the cases were observed. Thus, the nonmalignant cases constitute a majority. PSA, a marker specific for prostatic conditions, was significantly high in all the diseases compared to controls. A rise in serum PSA and PAP indicates prostatitis or, in combination with these two tests, decreased serum calcium shows advanced disease. PMID:28168057

  4. Phytotherapeutic Agents for Benign Prostatic Hyperplasia: An Overview.

    Science.gov (United States)

    Passi, Neelima Dhingra

    2016-06-20

    Benign prostatic hyperplasia (BPH) the most common condition in aging men is the non malignant enlargement of the prostate gland with increase in numbers of both epithelial and stromal cells within the periurethal transition zone of the prostate. Sources of symptoms in patient with BPH appear to be both static and dynamic component. Management of BPH has undergone a rapid evolution over the past decade to aid men with lower urinary tract symptoms attributed to bladder outlet obstruction. Treatment of clinical BPH aims to improve symptoms, prevent urinary tract infections, avoid renal insult, relief obstruction and improve bladder emptying.Prostate cancer patients and those with benign prostatic hyperplasia are increasingly exploring the use of plant derived non-nutritive compounds with protective or disease preventive properties, especially due to long term side effects of pharmacological treatment and risk of mortality associated with surgical procedures. Phytotherapeutic preparations are plant extracts with different components obtained by different extraction procedures. Numerous mechanisms of action have been postulated for mono and combination plant extracts. This article give a brief account of rationale and efficacy of various existing phytotherapeutic agents in the management of benign prostatic hyperplasia, including the herbs which hold the potential promise are also mentioned , although much research is still required.

  5. Testosterone metabolism of fibroblasts grown from prostatic carcinoma, benign prostatic hyperplasia and skin fibroblasts

    Energy Technology Data Exchange (ETDEWEB)

    Schweikert, H.U.; Hein, H.J.; Romijn, J.C.; Schroeder, F.H.

    1982-02-01

    The metabolism of (1,2,6,7-3H)testosterone was assessed in fibroblast monolayers derived from tissue of 5 prostates with benign hyperplasia (BPH), 4 prostates with carcinoma (PC), and 3 biopsy samples of skin, 2 nongenital skin (NG) and 1 genital skin. The following metabolites could be identified: androstanedione androstenedione, dihydrotestosterone, androsterone, epiandrosterone, androstane-3 alpha, 17 beta-diol and androstane-3 beta, 17 beta-diol. Testosterone was metabolized much more rapidly in fibroblasts originating from prostatic tissue than in fibroblasts derived from NG. A significantly higher formation of 5 alpha-androstanes and 3 alpha-hydroxysteroids could be observed in fibroblasts from BPH as compared to PC. 17-ketosteroid formation exceeded 5 alpha-androstane formation in BPH, whereas 5 alpha-reduction was the predominant pathway in fibroblasts grown from PC and NG. Since testosterone metabolism in fibroblasts of prostatic origin therefore resembles in many aspects that in whole prostatic tissue, fibroblasts grown from prostatic tissues might be a valuable tool for further investigation of the pathogenesis of human BPH and PC.

  6. Negative control of epithelial cell proliferation by prostatic stroma

    NARCIS (Netherlands)

    A. Kooistra (Anko)

    1995-01-01

    textabstractProstatic diseases are rather frequently occurring disorders in the male population. Prostatic adenoma -better known as benign prostatic hyperplasia (BPH)- is the most common benign neoplasm in men. Prostate cancer is the most commonly diagnosed malignancy in men and second leading cause

  7. Bio-effects of near-zero magnetic fields on the growth, development and reproduction of small brown planthopper, Laodelphax striatellus and brown planthopper, Nilaparvata lugens.

    Science.gov (United States)

    Wan, Gui-jun; Jiang, Shou-lin; Zhao, Zong-chao; Xu, Jing-jing; Tao, Xiao-rong; Sword, Gregory A; Gao, Yue-bo; Pan, Wei-dong; Chen, Fa-jun

    2014-09-01

    Magnetic fields markedly affect the growth and development of many species of organisms potentially due to cryptochrome and endogenous presence of magnetic materials. Sensitivity to magnetic fields can also be involved in geomagnetic orientation by some long-distance migratory insects. In this study, near-zero magnetic fields (NZMF) in relation to normal geomagnetic fields (GMF) were setup using the Hypomagnetic Field Space System (HMFs) to investigate the effects of magnetic fields on the growth, development and reproduction of two species of migratory planthopper, the small brown planthopper (abbr. SBPH), Laodelphax striatellus, and the brown planthopper (abbr. BPH), Nilaparvata lugens. Exposure of both L. striatellus and N. lugens to NZMF delayed egg and nymphal developmental durations and decreased adult weight and female fecundity. The 1st-5th instars of SBPH and BPH showed different responses to NZMF. The 4th instar was significantly affected by NZMF, especially for BPH males, in which NZMF exposure reduced the difference in development duration between females and males. Compared with GMF, the vitellogenin transcript levels of newly molted female adults and the number of eggs per female were significantly reduced in both planthopper species, indicating a negative effect on fertility under NZMF. Our findings provided experimental evidence that NZMF negatively affected the growth and development of SBPH and BPH, with particularly strong effects on reproduction.

  8. The medical management of lower urinary tract symptoms and benign prostatic hyperplasia.

    Science.gov (United States)

    Holtgrewe, H L

    1998-11-01

    Prostatism is a widely used term assigned to the symptom complex of older men with voiding dysfunction. The cause of the syndrome has routinely been ascribed to an enlarged prostate. More recent thinking recognizes that many men with such symptoms do not, in fact, have prostate enlargement or benign prostatic hyperplasia (BPH) and that such symptoms are not a surrogate for BPH. Such recognition is essential if cost effective medical management of lower urinary tract symptoms (LUTS) is to be achieved. Prostate volume has emerged as a key factor in the selection of medical therapy of LUTS and BPH not only regarding symptom relief but also to the newer concept of the prevention of disease progression and the avoidance of future adverse events in those men with true BPH. In the United States, medical management is now first line therapy for LUTS. The proper selection of therapy based on the patient's individual pathophysiologic characteristics is now made possible by many new recent studies within the medical literature.

  9. Correlation of the American Urological Association symptom index with self-administered versions of the Madsen-Iversen, Boyarsky and Maine Medical Assessment Program symptom indexes. Measurement Committee of the American Urological Association.

    Science.gov (United States)

    Barry, M J; Fowler, F J; O'Leary, M P; Bruskewitz, R C; Holtgrewe, H L; Mebust, W K

    1992-11-01

    We correlated the American Urological Association (AUA) symptom index with other indexes that have been used to measure symptoms for benign prostatic hyperplasia (BPH) and compared their psychometric properties. A self-administered questionnaire that allowed derivation of AUA, Maine Medical Assessment Program, Madsen-Iversen and Boyarsky symptom scores was completed by 76 men with clinically defined BPH, 59 younger control subjects, and 27 men before and after prostatectomy. The scores from the 4 indexes were strongly correlated (r = 0.77 to 0.93). All 4 indexes had good internal consistency and test-retest reliabilities. All indexes were predictive of patient global ratings of the degree of bother from the urinary condition. The AUA index discriminated BPH patients from controls significantly better than the Maine Medical Assessment Program index, and equivalently to the Madsen-Iversen and Boyarsky indexes (despite having fewer items). All 4 indexes were responsive when BPH patients underwent prostatectomy, although the AUA and Madsen-Iversen indexes were significantly more sensitive.

  10. Prostate-specific antigen as an estimator of prostate volume in the management of patients with symptomatic benign prostatic hyperplasia

    NARCIS (Netherlands)

    Mochtar, CA; Kiemeney, LALM; van Riemsdijk, MM; Barnett, GS; Laguna, MP; Debruyne, FMJ; de la Rosette, JJMCH

    2003-01-01

    Objectives: To assess the ability of serum prostate specific antigen (PSA) to estimate prostate volume (PV) to aid in the management of patients with benign prostatic hyperplasia (BPH). Methods: From 1989 to 2002, data were collected from 2264 patients complaining of lower urinary tract symptoms (LU

  11. Prazolin as a treatment for benign prostatic hypertrophy: a study in a tertiary care centre

    Directory of Open Access Journals (Sweden)

    Donepudi Pavan Kumar

    2016-06-01

    Conclusions: We therefore conclude that prazosin is also a very effective drug for the treatment of BPH, in terms of the urine outflow and decreased frequency and nocturia. [Int J Basic Clin Pharmacol 2016; 5(3.000: 639-642

  12. Cloning and characterization of rice RH3 gene induced by brown planthopper

    Institute of Scientific and Technical Information of China (English)

    WANG Xiaolan; WENG Qingmei; YOU Aiqing; ZHU Lili; HE Guangcun

    2003-01-01

    Experiments have showed that the histone H3 gene is correlated with development, cell speciality and stress response. The RH3 full-length cDNA was isolated from the cDNA library of rice infested by brown planthopper (BPH) with EST (Accession no. BU572343) screened from rice SSH library as probe. This gene encodes histone H3 protein including 136 amino acids, with one amino acid different from a kind of disease resistance-related protein in rice (AF467728). At the position 126, the aspartic acid is replaced by lysine. The time course results showed that the expression of the RH3 began to increase at 8 h after BPH-feeding, and got to its peak at 96 h. Regulations of the gene expression in treatments with stress/defense signal molecules were analyzed by Northern blot. Water deficit and Pyricularia grisea increased the expression of RH3 while ABA down-regulated the gene. The enhanced accumulation of RH3 transcripts in the vascular bundle and short cell of stem after BPH feeding was revealed by RNA in situ hybridization. It is the first time to report that RH3 is correlated with the response of rice to BPH.

  13. Insecticide resistance selection in rice planthoppers

    Institute of Scientific and Technical Information of China (English)

    2001-01-01

    @@ Brown planthopper (BPH, Nilaparvata lugens Stal) and white backed planthopper (WBPH, Sogatella furcifera Horvath) are the main insects on rice in China. The insecticide resistance of the two planthoppers have often been reported. Availability of the resistant population is a prerequisite for studying the resistance mechanism. In this paper, one method to select methamidophos resistance of the two planthoppers was recommended.

  14. The insecticide resistance in two planthoppers from three areas to three insecticides

    Institute of Scientific and Technical Information of China (English)

    2002-01-01

    @@ Migrating insects brown planthopper (BPH), Nilaparata lugens Stal and white-backed planthopper (WBPH), Sogatellafurcifera Horvath are both most harmful insects on rice in China. Chemical control is thought to be the best way to manage them, but it may cause insecticide resistance.

  15. Clinical significance of the leptin and leptin receptor expressions in prostate tissues

    Institute of Scientific and Technical Information of China (English)

    Jung Hoon Kim; Shin Young Lee; Soon Chul Myung; Young Sun Kim; Tae-Hyoung Kim; Mi Kyung Kim

    2008-01-01

    Aim: To evaluate the expression of leptin and leptin receptor in benign prostatic hyperplasia (BPH) and prostate cancer (Pca), and to investigate whether they are associated with the development and progression of Pca. Methods:Immunohistochemical staining was performed to examine the expression of leptin and leptin receptor in BPH and Pca.Pca was divided into three groups: localized Pca, locally advanced Pca and metastatic Pca. The positive staining was identified and the percentage of the positive staining was graded. We also assessed the relationship between both the Gleason score and body mass index (BMI) and Pca. Results: The percentage of the leptin expression in Pca was significantly higher than that in BPH (P < 0.01). For the Pca group, the expressed levels of leptin showed a considerable correlation with localized Pca and metastatic Pca (P < 0.05). Leptin receptor, however, did not reveal a definite difference between BPH and Pca. The expression of leptin indicated a significant difference between well-differen-tiated Pca (Gleason score < 6) and poorly differentiated Pca (Gleason score 8-10) (P < 0.05), The relation between the leptin expression level in Pca and the BMI was not remarkable (P = 0.447). Conclusion: Our results suggest that leptin might have a promoting effect on the carcinogenesis and progression of Pca.

  16. Occupational physical activity in relation with prostate cancer and benign prostatic hyperplasia.

    Science.gov (United States)

    Lagiou, Areti; Samoli, Evi; Georgila, Christina; Minaki, Ploumi; Barbouni, Anastasia; Tzonou, Anastasia; Trichopoulos, Dimitrios; Lagiou, Pagona

    2008-08-01

    Using data from two case-control studies undertaken in Athens, Greece from 1994 to 1997, we have examined the association of occupational physical activity with the risk of prostate cancer and benign prostatic hyperplasia (BPH). Cases consisted of 320 patients with histologically confirmed incident prostate cancer and 184 patients with surgically treated BPH. Controls were 246 patients hospitalized for minor conditions. Occupations before retirement were classified, independently and blindly as to case-control status, into high, medium, and low physical activity levels. After fine controlling for years of schooling, there was a suggestive inverse association of physical activity with prostate cancer (P for trend 0.12) and a significant one with BPH (P for trend 0.04). The odds ratio (95% confidence interval) for high versus low activity was 0.69 (0.40-1.22) for prostate cancer and 0.59 (0.31-1.11) for BPH. The association of physical activity with both conditions tended to be more pronounced among men 65 years old or younger. Given the high frequency of occurrence of the examined conditions in the male population and our limited knowledge about other modifiable risk factors, preventive measures may have to focus on increasing physical activity.

  17. [Lower urinary tract symptoms and male sexual dysfunction: the multinational survey of the aging.male (MSAM-7)

    NARCIS (Netherlands)

    Rosen, R.; Altwein, J.; Boyle, P.; Kirby, R.S.; Lukacs, B.; Meuleman, E.J.H.; O'Leary, M.; Puppo, P.; Chris, R.; Giuliano, F.

    2004-01-01

    OBJECTIVES: Lower urinary tract symptoms (LUTS), which are often caused by benign prostatic hypertrophy (BPH), and sexual dysfunction are common in older men, with an overall prevalence of > 50% in men aged > 50 years. Men with LUTS have been reported to experience sexual dysfunction, includin

  18. Jasmonic acid carboxyl methyltransferase regulates development and herbivory-induced defense response in rice

    Institute of Scientific and Technical Information of China (English)

    Jinfeng Qi; Yonggen Lou; Jiancai Li; Xiu Han; Ran Li; Jianqiang Wu; Haixin Yu; Lingfei Hu; Yutao Xiao; Jing Lu

    2016-01-01

    Jasmonic acid (JA) and related metabolites play a key role in plant defense and growth. JA carboxyl methyltransferase (JMT) may be involved in plant defense and development by methylating JA to methyl jasmonate (MeJA) and thus influencing the concentrations of JA and related metabolites. However, no JMT gene has been well characterized in monocotyledon defense and development at the molecular level. After we cloned a rice JMT gene, OsJMT1, whose encoding protein was localized in the cytosol, we found that the recombinant OsJMT1 protein catalyzed JA to MeJA. OsJMT1 is up-regulated in response to infestation with the brown planthopper (BPH; Nilaparvata lugens). Plants in which OsJMT1 had been overexpressed (oe-JMT plants) showed reduced height and yield. These oe-JMT plants also exhibited increased MeJA levels but reduced levels of herbivore-induced JA and jasmonoyl-isoleucine (JA-Ile). The oe-JMT plants were more attractive to BPH female adults but showed increased resistance to BPH nymphs, probably owing to the different responses of BPH female adults and nymphs to the changes in levels of H2O2 and MeJA in oe-JMT plants. These results indicate that OsJMT1, by altering levels of JA and related metabolites, plays a role in regulating plant development and herbivore-induced defense responses in rice.

  19. Prospective, Randomized, Multinational Study of Prostatic Urethral Lift Versus Transurethral Resection of the Prostate

    DEFF Research Database (Denmark)

    Sønksen, Jens; Barber, Neil J; Speakman, Mark J

    2015-01-01

    BACKGROUND: Transurethral resection of the prostate (TURP) is considered the gold standard for male lower urinary tract symptoms (LUTS) secondary to benign prostatic hyperplasia (BPH). However, TURP may lead to sexual dysfunction and incontinence, and has a long recovery period. Prostatic urethral...

  20. Reasons for the weak correlation between prostate volume and urethral resistance parameters in patients with prostatism

    NARCIS (Netherlands)

    J.L.H.R. Bosch (Ruud); R. Kranse (Ries); R. van Mastrigt (Ron); F.H. Schröder (Fritz); W.K. Mebust (W.)

    1995-01-01

    textabstractIn an attempt to increase our understanding of the clinical syndrome of benign prostatic hyperplasia (BPH) an analysis was made of the association between prostate volume as measured by transrectal ultrasound and several reported urodynamically determined urethral resistance parameters.

  1. Update on the use of dutasteride in the management of benign prostatic hypertrophy

    Directory of Open Access Journals (Sweden)

    Joe Miller

    2007-04-01

    Full Text Available Joe Miller, Thomas H TarterDivision of Urology, Southern Illinois University School of Medicine, Springfield, IL, USAAbstract: Benign prostatic hyperplasia (BPH is a frequent cause of lower urinary symptoms, with a prevalence of 50% by the sixth decade of life. Hyperplasia of stromal and epithelial prostatic elements that surround the urethra cause lower urinary tract symptoms (LUTS, urinary tract infection, and acute urinary retention. Medical treatments of symptomatic BPH include; 1 the 5α-reductase inhibitors, 2 the α1-adrenergic antagonists, and 3 the combination of a 5α-reductase inhibitor and a α1-adrenergic antagonist. Selective α1-adrenergic antagonists relax the smooth muscle of the prostate and bladder neck without affecting the detrussor muscle of the bladder wall, thus decreasing the resistance to urine flow without compromising bladder contractility. Clinical trials have shown that α1-adrenergic antagonists decrease LUTS and increase urinary flow rates in men with symptomatic BPH, but do not reduce the long-term risk of urinary retention or need for surgical intervention. Inhibitors of 5α-reductase decrease production of dihydrotestosterone within the prostate resulting in decreased prostate volumes, increased peak urinary flow rates, improvement of symptoms, and decreased risk of acute urinary retention and need for surgical intervention. The combination of a 5α-reductase inhibitor and a α1-adrenergic antagonist reduces the clinical progression of BPH over either class of drug alone.Keywords: prostatic hyperplasia, 5α-reductase, dutasteride

  2. A signaling network in phenylephrine-induced benign prostatic hyperplasia.

    Science.gov (United States)

    Kim, Jayoung; Yanagihara, Yutaka; Kikugawa, Tadahiko; Ji, Mihee; Tanji, Nozomu; Masayoshi, Yokoyama; Freeman, Michael R

    2009-08-01

    Benign prostatic hyperplasia (BPH) is an age-related disease of unknown etiology characterized by prostatic enlargement and coinciding with distinctive alterations in tissue histomorphology. To identify the molecular mechanisms underlying the development of BPH, we conducted a DNA microarray study using a previously described animal model in which chronic alpha(1)-adrenergic stimulation by repeated administration of phenylephrine evokes histomorphological changes in the rat prostate that resemble human BPH. Bioinformatic tools were applied to microarray data obtained from prostate tissue to construct a network model of potentially relevant signal transduction pathways. Significant involvement of inflammatory pathways was demonstrable, including evidence for activation of a TGF-beta signaling cascade. The heterodimeric protein clusterin (apolipoprotein J) was also identified as a prominent node in the network. Responsiveness of TGF-beta signaling and clusterin gene and protein expression were confirmed independently of the microarray data, verifying some components of the model. This is the first attempt to develop a comprehensive molecular network for histological BPH induced by adrenergic activation. The study also implicated clusterin as a novel biochemical target for therapy.

  3. Cholesterol and benign prostate disease.

    Science.gov (United States)

    Freeman, Michael R; Solomon, Keith R

    2011-01-01

    The origins of benign prostatic diseases, such as benign prostatic hyperplasia (BPH) and chronic prostatitis/chronic pelvic pain syndrome (CP/CPPS), are poorly understood. Patients suffering from benign prostatic symptoms report a substantially reduced quality of life, and the relationship between benign prostate conditions and prostate cancer is uncertain. Epidemiologic data for BPH and CP/CPPS are limited, however an apparent association between BPH symptoms and cardiovascular disease (CVD) has been consistently reported. The prostate synthesizes and stores large amounts of cholesterol and prostate tissues may be particularly sensitive to perturbations in cholesterol metabolism. Hypercholesterolemia, a major risk factor for CVD, is also a risk factor for BPH. Animal model and clinical trial findings suggest that agents that inhibit cholesterol absorption from the intestine, such as the class of compounds known as polyene macrolides, can reduce prostate gland size and improve lower urinary tract symptoms (LUTS). Observational studies indicate that cholesterol-lowering drugs reduce the risk of aggressive prostate cancer, while prostate cancer cell growth and survival pathways depend in part on cholesterol-sensitive biochemical mechanisms. Here we review the evidence that cholesterol metabolism plays a role in the incidence of benign prostate disease and we highlight possible therapeutic approaches based on this concept.

  4. Characteristics of Oxidative Storage Stability of Canola Fatty Acid Methyl Ester Stabilised with Antioxidants

    Directory of Open Access Journals (Sweden)

    Tirto Prakoso

    2012-11-01

    Full Text Available The storage effects on the oxidation characteristics of fatty acid methyl ester of canola oil (CME were investigated in this study. CME stabilised with two antioxidants, i.e. 2,6-di-tert-bytyl-p-cresol (BHT and 6,6-di-tert-butyl-2, 2’-methylendi-p-cresol (BPH, was stored at 20, 40 and 60°C. The oxidation stability data were measured by the Rancimat test method and it was found that both BHT and BPH addition increased the oxidation resistance of the CME. The results showed that when BPH or BHT was added at a concentration of 100 ppm, the oxidation induction period of the neat CME samples increased from 5.53 h to 6.93 h and 6.14 h, respectively. Comparing both antioxidants, BPH proved to be more effective in increasing the oxidation resistance when both antioxidants were added at the same concentration. Furthermore, the oxidation induction time decreased linearly with the storage time. It was shown that the oxidation occurred rapidly in the first 8 weeks of storage. Later, a kinetic study was undertaken and first-order kinetics were applied to explain the oxidation characteristics of the CME added with antioxidants. This kinetic study focused on exploiting the activation energy values obtained from the Arrhenius equations. Also, the oxidation effects on other quality parameters, including acid value, peroxide value, kinematic viscosity, and water content, were examined.

  5. Intraprostatic Botulinum Neurotoxin Type A Injection for Benign Prostatic Hyperplasia—A Spotlight in Reality

    Directory of Open Access Journals (Sweden)

    Yu-Chao Hsu

    2016-04-01

    Full Text Available Botulinum toxin is a neurotoxin produced by the bacterium Clostridium botulinum. It inhibits the release of acetylcholine and other neurotransmitters from the nerve terminal. Botulinum toxin, specifically toxin type A (BoNT-A has been used since the 1970s to reduce the muscular hypercontraction disorders. The application of BoNT-A in urology field started from intra-bladder injection for overactive bladder, which has been recognized as third line therapy in many countries. Since prostate gland as well as bladder is under the influence of autonomic innervation, theorectically, injection of BoNT-A into the prostate induces chemo-denervation and modulation of prostate function, and reduces lower urinary tract symptoms (LUTS. This article reviews the application of BoNT-A in patients with LUTS/ benign prostatic hyperplasia (BPH from mechanisms of action to clinical results. BoNT-A has been shown to induce prostate apoptosis, downregulation of alpha 1A receptors, and reduce contractile function of prostate in animal studies. Open studies of intraprostate BoNT-A injection have demonstrated promising results of reducing LUTS and improvement of voiding function in human LUTS/BPH, however, intraprostatic BoNT-A injection did not perform better than the placebo group in recent publications of placebo controlled studies. We suggested that BoNT-A prostate injection might benefit selected population of BPH/LUTS, but it is unlikely to be an effective therapy for general population of male LUTS/BPH.

  6. A T-shape diphosphinoborane palladium(0 complex

    Directory of Open Access Journals (Sweden)

    Patrick Steinhoff

    2016-07-01

    Full Text Available The reaction of CpPd(η3-C3H5 with the new diphosphinoborane ligand derivative (o-PCy2-C6H42BPh CyDPBPh affords the T-shape complex (CyDPBPhPd(0 9, which was characterized by X-ray analysis.

  7. Efficacy observation on benign prostatic hyperplasia treated with acupuncture and moxibustion

    Institute of Scientific and Technical Information of China (English)

    徐泽杰

    2014-01-01

    Objective To compare the difference in the clinical efficacy on benign prostatic hyperplasia(BPH)between the acupuncture-moxibustion therapy and the medication of Qianliekang tablets.Methods One hundred and twenty eight patients were randomized into an acupuncturemoxibustion group and a Qianliekang,64 cases in each.In the acupuncture-moxibustion group,acupuncture was

  8. Clinical Progress on Acupuncture Treatment of Prostatic Hyperplasia

    Institute of Scientific and Technical Information of China (English)

    李丽会; 张淑静; 徐名一; 黄国琪

    2009-01-01

    @@ Prostatic hyperplasia, also termed benign prostatic hyperplasia (BPH), is a commonly encountered disease in middle-aged and old males. This condition is known as "Retention of Urine" and "Urination Syndrome" in Chinese medicine. Now, the literature from 1998 to 2008 on acupuncture treatment of prostatic hyperplasia are abstracted and reviewed, in order to understand the clinical status of acupuncture treatment for this disease.

  9. Biomarkers of Prostatic Cancer: An Attempt to Categorize Patients into Prostatic Carcinoma, Benign Prostatic Hyperplasia, or Prostatitis Based on Serum Prostate Specific Antigen, Prostatic Acid Phosphatase, Calcium, and Phosphorus

    Directory of Open Access Journals (Sweden)

    Shahana Sarwar

    2017-01-01

    Full Text Available Prostatitis, BPH, and P.Ca are the most frequent pathologies of the prostate gland that are responsible for morbidity in men. Raised levels of PSA are seen in different pathological conditions involving the prostate. PAP levels are altered in inflammatory or infectious or abnormal growth of the prostate tissue. Serum calcium and phosphorus levels were also found to be altered in prostate cancer and BPH. The present study was carried out to study the levels of PSA, PAP, calcium, and phosphorus in serum of patients with Prostatitis, BPH, or P.Ca and also to evaluate the relationship between them. Males in the age group of 50–85 years with LUTS disease symptoms and with PSA levels more than 4 ng/mL were included. A total of 114 patients were analyzed including 30 controls. Prostatitis in 35.7% of cases, BPH in 35.7% of the cases, and P.Ca in 28.57% of the cases were observed. Thus, the nonmalignant cases constitute a majority. PSA, a marker specific for prostatic conditions, was significantly high in all the diseases compared to controls. A rise in serum PSA and PAP indicates prostatitis or, in combination with these two tests, decreased serum calcium shows advanced disease.

  10. The role of transurethral resection of the prostate for patients with an elevated prostate-specific antigen

    Directory of Open Access Journals (Sweden)

    Hee Ju Cho

    2014-12-01

    Conclusions: TURP significantly reduced the serum PSA level, which was maintained for at least 3 years. This could be helpful to screen the prostate cancer using PSA value in the patient with previous negative biopsy and elevated PSA. In addition, TURP improves IPSS, Qmax, and PVR in patients with BPH, moderate LUTS, and an elevated PSA level.

  11. Inflammation et hyperplasie bénigne de la prostate : cause ou conséquence?

    NARCIS (Netherlands)

    Robert, G.Y.M.; Salagierski, M.; Schalken, J.A.; Taille, A. De La

    2010-01-01

    INTRODUCTION AND OBJECTIVES: Although benign prostatic hyperplasia (BPH) is the most frequent disease in elderly men, only a few predictive factors have been clearly identified. Recently, chronic prostatic inflammation has emerged as one of them. This review aims at describing the scientific proof o

  12. Catalytic performance and molecular dynamic simulation of immobilized CC bond hydrolase based on carbon nanotube matrix.

    Science.gov (United States)

    Zhou, Hao; Qu, Yuanyuan; Kong, Chunlei; Li, Duanxing; Shen, E; Ma, Qiao; Zhang, Xuwang; Wang, Jingwei; Zhou, Jiti

    2014-04-01

    Carbon nanotube (CNT) has been proved to be a kind of novel support for enzyme immobilization. In this study, we tried to find the relationship between conformation and catalytic performance of immobilized enzyme. Two CC bond hydrolases BphD and MfphA were immobilized on CNTs (SWCNT and MWCNT) via physical adsorption and covalent attachment. Among the conjugates, the immobilized BphD on chemically functionalized SWCNT (BphD-CSWCNT) retained the highest catalytic efficiency (kcat/Km value) compared to free BphD (92.9%). On the other hand, when MfphA bound to pristine SWCNT (MfphA-SWCNT), it was completely inactive. Time-resolved fluorescence spectrum indicated the formation of static ground complexes during the immobilization processes. Circular dichroism (CD) showed that the secondary structures of immobilized enzymes changed in varying degrees. In order to investigate the inhibition mechanism of MfphA by SWCNT, molecular dynamics simulation was employed to analyze the adsorption process, binding sites and time evolution of substrate tunnels. The results showed that the preferred binding sites (Trp201 and Met81) of MfphA for SWCNT blocked the main substrate access tunnel, thus making the enzyme inactive. The "tunnel-block" should be a novel possible inhibition mechanism for enzyme-nanotube conjugate.

  13. Blockade of Androgen Markers Using a Novel Betasitosterol, Thioctic Acid and Carnitine-containing Compound in Prostate and Hair Follicle Cell-based Assays.

    Science.gov (United States)

    Chen, Li; Wang, Jiaolong; Mouser, Glen; Li, Yan Chun; Marcovici, Geno

    2016-06-01

    Androgenetic alopecia (AGA) affects approximately 70% of men and 40% of women in an age-dependent manner and is partially mediated by androgen hormones. Benign prostatic hyperplasia (BPH) similarly affects 50% of the male population, rising by 10% each decade. Finasteride inhibits 5-alpha reductase (5AR) and is used to treat both disorders, despite offering limited clinical benefits accompanied by significant adverse side effects. Building on our previous work demonstrating the efficacy of naturally derived 5AR inhibitors (such as stigmasterol and beta sitosterol), we hypothesize that targeting 5AR as well as inflammatory pathways may yield improved efficacy in AGA and BPH. Here we address these dual pathomechanisms by examining the potency of a novel composition using in vitro assays of representative cell lines for AGA (hair follicle dermal papilla cells) and BPH (LNCaP prostate cells), respectively. Exposure of cells to the novel test composition down-regulated mRNA expression profiles characteristic of both disease processes, which outperformed finasteride. Changes in mRNA expression were corroborated at the protein level as assessed by western blotting. These studies provide proof of concept that novel, naturally derived compositions simultaneously targeting 5AR and inflammatory mediators may represent a rational approach to treating AGA and BPH. Copyright © 2016 John Wiley & Sons, Ltd.

  14. Naked (C5Me5)(2)M cations (M = Sc, Ti, and V) and their fluoroarene complexes

    NARCIS (Netherlands)

    Bouwkamp, MW; Budzelaar, PHM; Gercama, J; Morales, ID; de Wolf, J; Meetsma, A; Troyanov, SI; Teuben, JH; Hessen, B; Budzelaar, Peter H.M.; Hierro Morales, Isabel Del; Troyanov, Sergei I.

    2005-01-01

    The ionic metallocene complexes [Cp*M-2][BPh4] (CP* = C5Me5) of the trivalent 3d metals Sc, Ti, and V were synthesized and structurally characterized. For M Sc, the anion interacts weakly with the metal center through one of the phenyl groups, but for M = Ti and V, the cations are naked. They each c

  15. Resistance to green leafhopper (Nephotettix virescens) and brown planthopper (Nilaparvata lugens) in transgenic rice expressing snowdrop lectin (Galanthus nivalis agglutinin; GNA).

    Science.gov (United States)

    Foissac, X; Thi Loc, N; Christou, P; Gatehouse, A M.R.; Gatehouse, J A.

    2000-04-01

    Transgenic rice plants expressing snowdrop lectin (Galanthus nivalis agglutinin; GNA) were screened for resistance to green leafhopper (Nephotettix virescens; GLH), a major homopteran pest of rice. Survival was reduced by 29% and 53% (P<0.05) respectively, on plants where GNA expression was tissue-specific (phloem and epidermal layer) or constitutive. Similar levels of resistance in GNA-expressing transgenic rice were previously reported for rice brown planthopper (Nilaparvata lugens; BPH). GNA binding to glycoproteins in gut tissues showed that BPH contained more "receptors" than GLH, and that the binding affinity was stronger, particularly in the midgut. Subsequent toxicity of GNA is thus unlikely to be directly related to the amount of lectin bound. GNA was not detected in the honeydew of either insect species when they were fed on GNA-expressing plants, in contrast to results from artificial diet studies. This result suggests that GNA is not being delivered to the insect efficiently. When offered a free choice vs control plants, BPH nymphs tended to avoid plants expressing GNA; avoidance was less pronounced and took longer to develop on plants where GNA expression was tissue-specific, In contrast to BPH, GLH nymphs were attracted to plants expressing GNA, whether constitutively or in a tissue-specific manner.

  16. Expression of snowdrop lectin (GNA) in transgenic rice plants confers resistance to rice brown planthopper.

    Science.gov (United States)

    Rao, K V; Rathore, K S; Hodges, T K; Fu, X; Stoger, E; Sudhakar, D; Williams, S; Christou, P; Bharathi, M; Bown, D P; Powell, K S; Spence, J; Gatehouse, A M; Gatehouse, J A

    1998-08-01

    Snowdrop lectin (Galanthus nivalis agglutinin; GNA) has been shown previously to be toxic towards rice brown planthopper (Nilaparvata lugens; BPH) when administered in artificial diet. BPH feeds by phloem abstraction, and causes 'hopper burn', as well as being an important virus vector. To evaluate the potential of the gna gene to confer resistance towards BPH, transgenic rice (Oryza sativa L.) plants were produced, containing the gna gene in constructs where its expression was driven by a phloem-specific promoter (from the rice sucrose synthase RSs1 gene) and by a constitutive promoter (from the maize ubiquitin ubi1 gene). PCR and Southern analyses on DNA from these plants confirmed their transgenic status, and that the transgenes were transmitted to progeny after self-fertilization. Western blot analyses revealed expression of GNA at levels of up to 2.0% of total protein in some of the transgenic plants. GNA expression driven by the RSs1 promoter was tissue-specific, as shown by immunohistochemical localization of the protein in the non-lignified vascular tissue of transgenic plants. Insect bioassays and feeding studies showed that GNA expressed in the transgenic rice plants decreased survival and overall fecundity (production of offspring) of the insects, retarded insect development, and had a deterrent effect on BPH feeding. gna is the first transgene to exhibit insecticidal activity towards sap-sucking insects in an important cereal crop plant.

  17. Evidence for the presence of biogenic magnetic particles in the nocturnal migratory brown planthopper, Nilaparvata lugens.

    Science.gov (United States)

    Pan, Weidong; Wan, Guijun; Xu, Jingjing; Li, Xiaoming; Liu, Yuxin; Qi, Liping; Chen, Fajun

    2016-01-05

    Biogenic magnetic particles have been detected in some migratory insects, which implies the basis of magnetoreception mechanism for orientation and navigation. Here, the biogenic magnetic particles in the migratory brown planthopper (BPH), Nilaparvata lugens were qualitatively measured by SQUID magnetometry, and their characteristics were further determined by Prussian Blue staining, electron microscopy and energy dispersive x-ray spectroscopy. The results indicate that there were remarkable magnetic materials in the abdomens and not in the head or thorax of the 3(rd)-5(th) instar nymphs, and in macropterous and brachypterous female and male adults of BPH. The size of magnetic particles was shown to be between 50-450 nm with a shape factor estimate of between 0.8-1.0 for all the tested BPHs. Moreover, the amount of magnetic particles was associated with the developmental stage (the 3(rd)-5(th) instar), wing form (macropterous vs. brachypterous) and sex. The macropterous female adults had the largest amount of magnetic particles. Although the existence of magnetic particles in the abdomens of BPH provides sound basis for the assumption of magnetic orientation, further behavioral studies and complementary physical characterization experiments should be conducted to determine whether the orientation behavior of BPH is associated with the magnetic particles detected in this study.

  18. A T-shape diphosphinoborane palladium(0) complex

    Science.gov (United States)

    Steinhoff, Patrick

    2016-01-01

    Summary The reaction of CpPd(η3-C3H5) with the new diphosphinoborane ligand derivative (o-PCy2-C6H4)2BPh Cy DPB Ph affords the T-shape complex (Cy DPB Ph)Pd(0) 9, which was characterized by X-ray analysis. PMID:27559408

  19. Thermodynamic Properties of Transfer for Tetraphenylarsonium Tetraphylborate from Water to n—Alkanols

    Institute of Scientific and Technical Information of China (English)

    邹立壮; 路遥; 等

    2002-01-01

    Solubilities of tetraphenylarsonium tetraphenylborate(Ph4AsB-Ph4) in water,methanol,ethanol, 1-propanol,1-butanol,1-pentanol,1-hexanol and 1-octanol at T=293.2,298.2,303.2 and 308.2 K have been determined by spectrophotometry,The standard transfer Gibbs energy (△trG0w→s) and entropy (△trS0w→s) of Ph4AsBPh4 from water to the n-alkanols at temerature from 293.2 K to 308.2 K have been obtained.Fur-thermore,the contribution of microscopic interaction to the standard Gibbs energy of transfer for Ph4AsBPh4 was calculated and discussed,The results show that the effect of hydrophobic inderaction of Ph4AsBPh4 on its transfer process is the most im-portant factor .According to the thermodynamical principle,the transfer process of Ph4AsBPh4 from water to the n-alkanols is the entropy dominanted.

  20. Surgical therapy for benign prostatic hypertrophy/bladder outflow obstruction

    Directory of Open Access Journals (Sweden)

    Nikesh Thiruchelvam

    2014-01-01

    Full Text Available Monopolar transurethral resection of the prostate (TURP with endoscopic electrocautery remains the gold standard surgical technique for benign prostatic hypertrophy (BPH by which all new procedures are compared. We reviewed the current literature, and international urological guidelines and consensus opinion on various surgical options for BPH and present a brief overview of alternative techniques including bipolar TURP, transurethral incision of the prostate, transurethral vaporization of the prostate, laser prostatectomy (with holmium, thulium and potassium titanyl phosphate greenlight lasers and open prostatectomy (with mention of new techniques including laparoscopic and robotic prostatectomy. Emerging, experimental and less established techniques are also described including endoscopic heat generation (transurethral microwave thermotherapy, radiofrequency transurethral needle ablation of the prostate, high intensity focused ultrasound, hot water induced thermotherapy, pulsed electromagnetic radiofrequency, injection therapy (transurethral ethanol ablation and botulinum toxin and mechanical devices (intraprostatic stents and urethral lift devices. Despite a plethora of surgical options, none have realistically improved outcomes in the long-term compared with TURP. Improvements have been made on improving surgical morbidity and time in hospital. Questions remain in this area, including what specific elements of bladder outflow obstruction (BOO result in damage to the urinary tract, how does BPH contribute to BOO and how much prostate volume reduction is necessary to relieve BOO or lower urinary tract symptoms. Given these unanswered questions and the multitude of procedures available, it is clear that appropriate counselling is necessary in all men who undergo BPH surgery.

  1. Uroflowmetry in males. Refrence values and clinical application in benign prostatic hypertrophy.

    NARCIS (Netherlands)

    Rollema, Harm Jan

    1981-01-01

    In this thesis the results are presented of a computerassisted study on uroflowmetry in males. Chapter I introduces the aim of the study and gives a short reviw. Urinary outflow obstruction due to begign prostatic hypertrophy (BPH) is a slowly progressive disease that initially gives rise to a few s

  2. Clinical implications of free-to-total immunoreactive prostate-specific antigen ratios

    NARCIS (Netherlands)

    Wymenga, LFA; Duisterwinkel, FJ; Groenier, K; Visser-van Brummen, P; Marrink, J; Mensink, HJA

    2000-01-01

    Objective: A study was performed to evaluate the free-to-total prostate-specific antigen (PSA) ratio for discriminating benign prostatic hyperplasia (BPH) or prostate cancer in the intermediate PSA range (2.0-10.0 mu g/l) in patients referred for prostate evaluation. In addition, the relationship of

  3. Surgical therapy for benign prostatic hypertrophy/bladder outflow obstruction.

    Science.gov (United States)

    Thiruchelvam, Nikesh

    2014-04-01

    Monopolar transurethral resection of the prostate (TURP) with endoscopic electrocautery remains the gold standard surgical technique for benign prostatic hypertrophy (BPH) by which all new procedures are compared. We reviewed the current literature, and international urological guidelines and consensus opinion on various surgical options for BPH and present a brief overview of alternative techniques including bipolar TURP, transurethral incision of the prostate, transurethral vaporization of the prostate, laser prostatectomy (with holmium, thulium and potassium titanyl phosphate greenlight lasers) and open prostatectomy (with mention of new techniques including laparoscopic and robotic prostatectomy). Emerging, experimental and less established techniques are also described including endoscopic heat generation (transurethral microwave thermotherapy, radiofrequency transurethral needle ablation of the prostate, high intensity focused ultrasound, hot water induced thermotherapy, pulsed electromagnetic radiofrequency), injection therapy (transurethral ethanol ablation and botulinum toxin) and mechanical devices (intraprostatic stents and urethral lift devices). Despite a plethora of surgical options, none have realistically improved outcomes in the long-term compared with TURP. Improvements have been made on improving surgical morbidity and time in hospital. Questions remain in this area, including what specific elements of bladder outflow obstruction (BOO) result in damage to the urinary tract, how does BPH contribute to BOO and how much prostate volume reduction is necessary to relieve BOO or lower urinary tract symptoms. Given these unanswered questions and the multitude of procedures available, it is clear that appropriate counselling is necessary in all men who undergo BPH surgery.

  4. TISSUE POLYPEPTIDE-SPECIFIC ANTIGEN - A DISCRIMINATIVE PARAMETER BETWEEN PROSTATE-CANCER AND BENIGN PROSTATIC HYPERTROPHY

    NARCIS (Netherlands)

    MARRINK, J; OOSTEROM, R; BONFRER, HMG; SCHRODER, FH; MENSINK, HJA

    1993-01-01

    The serum concentration of the cell proliferation marker TPS (tissue polypeptide-specific antigen) was compared with the tumour marker PSA (prostate specific antigen). PSA was found elevated in 50% of the benign prostatic hypertrophy (BPH) patients, in 88% of the patients with active prostate cancer

  5. Update on the use of dutasteride in the management of benign prostatic hypertrophy.

    Science.gov (United States)

    Miller, Joe; Tarter, Thomas H

    2007-01-01

    Benign prostatic hyperplasia (BPH) is a frequent cause of lower urinary symptoms, with a prevalence of 50% by the sixth decade of life. Hyperplasia of stromal and epithelial prostatic elements that surround the urethra cause lower urinary tract symptoms (LUTS), urinary tract infection, and acute urinary retention. Medical treatments of symptomatic BPH include; 1) the 5alpha-reductase inhibitors, 2) the alpha1-adrenergic antagonists, and 3) the combination of a 5alpha-reductase inhibitor and a alpha1-adrenergic antagonist. Selective alpha1-adrenergic antagonists relax the smooth muscle of the prostate and bladder neck without affecting the detrussor muscle of the bladder wall, thus decreasing the resistance to urine flow without compromising bladder contractility. Clinical trials have shown that alpha1-adrenergic antagonists decrease LUTS and increase urinary flow rates in men with symptomatic BPH, but do not reduce the long-term risk of urinary retention or need for surgical intervention. Inhibitors of 5alpha-reductase decrease production of dihydrotestosterone within the prostate resulting in decreased prostate volumes, increased peak urinary flow rates, improvement of symptoms, and decreased risk of acute urinary retention and need for surgical intervention. The combination of a 5alpha-reductase inhibitor and a alpha1-adrenergic antagonist reduces the clinical progression of BPH over either class of drug alone.

  6. 17Beta-estradiol at low concentrations acts through distinct pathways in normal versus benign prostatic hyperplasia-derived prostate stromal cells.

    Science.gov (United States)

    Park, Irwin I; Zhang, Qiang; Liu, Victoria; Kozlowski, James M; Zhang, Ju; Lee, Chung

    2009-10-01

    The aim of this study was to identify differential responses to low concentrations of 17beta-estradiol (E2) in primary stromal cell cultures derived from either normal organ donors or benign prostatic hyperplasia or hypertrophy (BPH) specimens. Furthermore, we sought to identify the potential mechanism of E2 action in these cell types, through either a genomic or nongenomic mechanism. We initially treated stromal cells derived from five normal prostates or five BPH specimens with low concentrations of E2 (0.001-1.0 nM) and analyzed their growth response. To determine whether genomic or nongenomic pathways were involved, we performed studies using specific estrogen receptor antagonists to confirm transcriptional activity or MAPK inhibitors to confirm the involvement of rapid signaling. Results of these studies revealed a fundamental difference in the mechanism of the response to E2. In normal cells, we found that a nongenomic, rapid E2 signaling pathway is predominantly involved, mediated by G protein-coupled receptor-30 and the subsequent activation of ERK1/2. In BPH-derived prostate stromal cells, a genomic pathway is predominantly involved because the addition of ICI 182780 was sufficient to abrogate any estrogenic effects. In conclusion, prostate stromal cells respond to far lower concentrations of E2 than previously recognized or examined, and this response is mediated through two distinct mechanisms, depending on its origin. This may provide the basis for new insights into the causes of, and possible treatments for, BPH.

  7. Possible autocrine loop of the epidermal growth factor system in patients with benign prostatic hyperplasia treated with finasteride: a placebo-controlled randomized study

    DEFF Research Database (Denmark)

    Tørring, Niels; Jensen, Klaus Møller-Ernst; Lund, L;

    2002-01-01

    To analyse the expression of the epidermal growth factor (EGF) system in prostate tissue and secretions obtained from patients with benign prostatic hyperplasia (BPH) treated with or without finasteride (which primarily targets the androgen-sensitive secretory epithelial cells in the prostate...

  8. Evaluation of benign prostatic hyperplasia treatments : How can we improve the outcome measures and success criteria?

    NARCIS (Netherlands)

    Mensink, H

    1997-01-01

    Treatment evaluation in benign prostatic hyperplasia (BPH) is complicated by a number of interdependent factors. The evaluation of the individual patient is influenced by the risk:benefit ratio and health related quality of life and this, in turn, is interrelated with the evaluation of therapeutic o

  9. CURRENT OPPORTUNITIES FOR COMBINATION TREATMENT OF LOWER URINARY TRACT SYMPTOMS DUE TO BENIGN PROSTATIC HYPERPLASIA IN MEN

    Directory of Open Access Journals (Sweden)

    G. R. Kasyan

    2016-01-01

    Full Text Available Lower urinary tract symptoms (LUTS associated with benign prostatic hyperplasia (BPH are widely presented, particularly among elderly men. It was commonly accepted that LUTS are directly or indirectly connected with the prostate. Recent studies have shown that mild andsevere forms of LUTS may have a different etiology, such as, bladder related problems: overactive bladder (OAB/detrusor overactivity or, conversely, an underactive bladder due to compromised contractions of the detrusor. There are three main groups of LUTS: storage symptoms, voiding symptoms and post-micturition symptoms. Patients with LUTS/BPH who are not sufficiently respond to monotherapy with alpha-blockers should receive additional treatment with M‑anticholinergics in accordance with the European urological guidelines. Current studies show that up to 65% of patients with LUTS/BPH with symptoms of urgency and frequency do not have sufficient reduction of symptoms after 3 months of treatment with alphablocker as monotherapy. Until recently, alpha-blockers and M‑anticholinergic medications were prescribed in combination therapy as a two separate pills. Vesomni is a combination of antimuscarinic drug solifenacin 6 mg with α1‑blocker tamsulosin OCAS 0.4 mg in one tablet. It is developed to relieve storage (urgency and frequency and voiding symptoms in men with LUTS/BPH. Solifenacin suppresses the overactivity of the detrusor and reduces storage symptoms. Tamsulosin OCAS relaxes smooth muscle of the prostate, prostatic urethra and bladder neck, and eliminates most voiding symptoms and, to a lesser extent, storage symptoms.

  10. Age as a prognostic variable in patients undergoing transurethral prostatectomy

    DEFF Research Database (Denmark)

    Krogh, J; Jensen, J S; Iversen, H G;

    1993-01-01

    In a retrospective study the outcome of transurethral prostatectomy (TURP) for benign prostatic hyperplasia (BPH) in patients more than 80 years old was compared to a control group of patients with a mean age ten years younger. The elderly had significantly more tissue resected and presented...

  11. A systematic review of the effects and mechanisms of preoperative 5α-reductase inhibitors on intraoperative haemorrhage during surgery for benign prostatic hyperplasia

    Institute of Scientific and Technical Information of China (English)

    Huan-Tao Zong; Xiao-Xia Peng; Chen-Chen Yang; Yong Zhang

    2011-01-01

    5α-reductase inhibitors (5α-RIs),including finasteride and dutasteride,are commonly used medical therapies for benign prostatic hyperplasia (BPH).Many studies reported that preoperative 5α-RI had impact on intraoperative haemorrhage during surgery for BPH,but it was still in controversial.So,we conducted a systematic review of the effects and mechanisms of 5α-RIs on intraoperative bleeding for BPH.MEDLINE,EMBASE,the Cochrane Controlled Trail Register of Controlled Trials and the reference lists of retrieved studies were searched in the analysis.Sixteen publications involving 15 different randomized controlled trials (RCTs) and a total of 1156 patients were used in the analysis,including 10 RCTs for finasteride and five RCTs for dutasteride.We found that preoperative finasteride treatment decreases microvessel density (MVD) in resected prostate specimens.Total blood loss,blood loss per gram of resected prostate tissue and decreases in haemoglobin were all greatly reduced in the finasteride group as compared to controls.Dutasteride appeared to have no effect on bleeding.This meta-analysis shows that preoperative finasteride treatment could decrease intraoperative haemorrhage during surgery for BPH.Preoperative dutasteride had no effect on intraoperative haemorrhage,but further high-quality prospective studies are still needed to confirm this observation.

  12. Minimally invasive devices for treating lower urinary tract symptoms in benign prostate hyperplasia: technology update

    Directory of Open Access Journals (Sweden)

    Aoun F

    2015-08-01

    Full Text Available Fouad Aoun1, Quentin Marcelis,1,2 Thierry Roumeguère,2 1Department of Urology, Jules Bordet Institute, 2Department of Urology, Erasme Hospital, University Clinics of Brussels, Université Libre de Bruxelles, Brussels, Belgium Abstract: Benign prostatic hyperplasia (BPH represents a spectrum of related lower urinary tract symptoms (LUTS. The cost of currently recommended medications and the discontinuation rate due to side effects are significant drawbacks limiting their long-term use in clinical practice. Interventional procedures, considered as the definitive treatment for BPH, carry a significant risk of treatment-related complications in frail patients. These issues have contributed to the emergence of new approaches as alternative options to standard therapies. This paper reviews the recent literature regarding the experimental treatments under investigation and presents the currently available experimental devices and techniques used under local anesthesia for the treatment of LUTS/BPH in the vast majority of cases. Devices for delivery of thermal treatment (microwaves, radiofrequency, high-intensity focused ultrasound, and the Rezum system, mechanical devices (prostatic stent and urethral lift, fractionation of prostatic tissue (histotripsy and aquablation, prostate artery embolization, and intraprostatic drugs are discussed. Evidence for the safety, tolerability, and efficacy of these “minimally invasive procedures” is analyzed. Keywords: lower urinary tract symptoms (LUTS, benign prostatic hyperplasia (BPH, minimally invasive therapies, new approaches, experimental therapy

  13. Immunohistochemical profiles of claudin-3 in primary and metastatic prostatic adenocarcinoma

    Directory of Open Access Journals (Sweden)

    Becich Michael J

    2011-01-01

    Full Text Available Abstract Background Claudins are integral membrane proteins that are involved in forming cellular tight junctions. One member of the claudin family, claudin-3, has been shown to be overexpressed in breast, ovarian, and pancreatic cancer. Here we use immunohistochemistry to evaluate its expression in benign prostatic hyperplasia (BPH, prostatic intraepithelial neoplasia (PIN, normal tissue adjacent to prostatic adenocarcinoma (NAC, primary prostatic adenocarcinoma (PCa, and metastatic prostatic adenocarcinoma (Mets. Methods Tissue microarrays were immunohistochemically stained for claudin-3, with the staining intensities subsequently quantified and statistically analyzed using a one-way ANOVA with subsequent Tukey tests for multiple comparisons or a nonparametric equivalent. Fifty-three cases of NAC, 17 cases of BPH, 35 cases of PIN, 107 cases of PCa, and 55 cases of Mets were analyzed in the microarrays. Results PCa and Mets had the highest absolute staining for claudin-3. Both had significantly higher staining than BPH (p Conclusions To our knowledge, this represents one of the first studies comparing the immunohistochemical profiles of claudin-3 in PCa and NAC to specimens of PIN, BPH, and Mets. These findings provide further evidence that claudin-3 may serve as an important biomarker for prostate cancer, both primary and metastatic, but does not provide evidence that claudin-3 can be used to predict risk of metastasis.

  14. Outcome of the TURP-TUVP sandwich procedure for minimally invasive surgical treatment of benign prostatic hyperplasia with volume larger than 40cc over a 4-year period in Nigeria

    Directory of Open Access Journals (Sweden)

    O.I. Aisuodionoe-Shadrach

    2013-03-01

    Conclusions: The sandwich combination of TURP and TUVP for the surgical treatment of BPH with volume larger than 40cc had satisfactory patient safety profile and resulted in significant improvement in IPSS, Qmax and PVR on follow-up at 3 months.

  15. Proteomic analysis of formalin-fixed prostate cancer tissue.

    Science.gov (United States)

    Hood, Brian L; Darfler, Marlene M; Guiel, Thomas G; Furusato, Bungo; Lucas, David A; Ringeisen, Bradley R; Sesterhenn, Isabell A; Conrads, Thomas P; Veenstra, Timothy D; Krizman, David B

    2005-11-01

    Proteomic analysis of formalin-fixed paraffin-embedded (FFPE) tissue would enable retrospective biomarker investigations of this vast archive of pathologically characterized clinical samples that exist worldwide. These FFPE tissues are, however, refractory to proteomic investigations utilizing many state of the art methodologies largely due to the high level of covalently cross-linked proteins arising from formalin fixation. A novel tissue microdissection technique has been developed and combined with a method to extract soluble peptides directly from FFPE tissue for mass spectral analysis of prostate cancer (PCa) and benign prostate hyperplasia (BPH). Hundreds of proteins from PCa and BPH tissue were identified, including several known PCa markers such as prostate-specific antigen, prostatic acid phosphatase, and macrophage inhibitory cytokine-1. Quantitative proteomic profiling utilizing stable isotope labeling confirmed similar expression levels of prostate-specific antigen and prostatic acid phosphatase in BPH and PCa cells, whereas the expression of macrophage inhibitory cytokine-1 was found to be greater in PCa as compared with BPH cells.

  16. Risks of Prostate Cancer Screening

    Science.gov (United States)

    ... prostate may be similar to symptoms of prostate cancer . Enlarge Normal prostate and benign prostatic hyperplasia (BPH). A normal prostate does not block the flow of urine from the bladder. An enlarged prostate presses on the bladder and urethra and blocks the flow of urine. See the ...

  17. Enlarged prostate

    Science.gov (United States)

    ... prostate URL of this page: //medlineplus.gov/ency/article/000381.htm Enlarged prostate To use the sharing ... sperm during ejaculation. The prostate gland surrounds the urethra, the tube ... hyperplasia (BPH). It is not cancer, and it does not raise your risk for ...

  18. Placebo effects in the pharmacological treatment of uncomplicated benign prostatic hyperplasia

    NARCIS (Netherlands)

    Hansen, BJ; Meyhoff, HH; Nordling, J; Mensink, HJA; Mogensen, P; Larsen, EH; Leenarts, JAF; Oosten, JK; vanSoest, FF; Dijkman, GA; Hoekstra, JW; vanBaasbank, NJW; Bijleveld, RT; Braam, PFCM; Schlatmann, TJM; Felderhof, J; Kapper, BJ; Dik, P; Schou, J; Poulsen, AL; Christoffersen, J; Geerdsen, JP; Hvidt, [No Value; Dahl, C; Luke, M; Lendorph, A; Jacobsen, B; Bilde, T; Mortensen, S; Walter, S

    1996-01-01

    In order to establish accurately the exact effect of any drug therapy for symptomatic benign prostatic hyperplasia (BPH) it is important to define the effect of placebo treatment. This effect was assessed by thoroughly analyzing the placebo arm, which included 101 patients, from a randomized, double

  19. Functional and numerical responses ofCyrtorhinus lividipennis to eggs ofNilaparvata lugens are not affected by genetically modiifed herbicide-tolerant rice

    Institute of Scientific and Technical Information of China (English)

    JIANG Xian-bin; LONG Li-ping; HUANG Qian; LING Yan; CHEN Yu-chong; XIAO Guo-ying; HUANG Suo-sheng; WU Bi-qiu; HUANG Feng-kuan; CAI Jian-he

    2015-01-01

    To safely and sustainably utilize genetic breeding techniques for crop production, greater understanding of the potential effects of geneticaly modiifed herbicide-tolerant (GMHT) crops on the ecological functions of predators is required. In the laboratory, we examined the functional and numerical responses ofCyrtorhinus lividipennis Reuter to eggs of brown plan-thopper (BPH), Nilaparvata lugens (Stål), which were reared on GMHT rice Bar68-1; the untransformed parental cultivar, D68; or a BPH-susceptive rice variety, Taichung Native 1. Al stages of nymphs and female adultof C. lividipennis, either on GMHT rice or control plants, exhibited typical type II functional responses when fed on BPH eggs; the attacking rate and handling time ofC. lividipennis on GMHT rice Bar68-1 was not signiifcantly different from that on D68. The numerical responses ofC. lividipennis on GMHT rice or controls ift Beddington’s model; there were no signiifcant differences in the parameters of numerical responses between GMHT rice Bar68-1 and D68. The results indicated that the functional and numerical responses ofC. lividipennis to BPH eggs are not affected by GMHT rice Bar68-1.

  20. Evaluation of the clinical efficacy of Gonazon implants in the treatment of reproductive pathologies, behavioral problems, and suppression of reproductive function in the male dog.

    Science.gov (United States)

    Goericke-Pesch, S; Wilhelm, E; Ludwig, C; Desmoulins, P O; Driancourt, M A; Hoffmann, B

    2010-04-15

    Efficacy of a slow-release gonadotropin-releasing hormone (GnRH)-agonist implant (Gonazon) was assessed in 53 male dogs presented with benign prostatic hyperplasia (BPH), hypersexuality, aggressive behavior (either alone or in combination), excessive micturition, or to suppress fertility. Changes in testosterone (T) and estradiol (E2) concentrations and size of testes and prostate were monitored on Weeks 0, +8, and +26 after implantation. Additional measurements during and after this period were performed in 35 dogs. Clinical signs were assessed by the owners. All implants except one were retained throughout the study. Full downregulation of testicular function (Tdogs, five dogs showed partial downregulation (T = 0.36 to 0.47 nmol/L), one dog did not respond, and another one displayed a transient downregulation on Week +18. On Week +8, mean T and E2 levels were reduced by 96% and 62%, respectively, and did not further decrease. Full downregulation (T22 mo in most dogs except two. Compared with pretreatment values, mean testicular and prostatic size was reduced (Pdogs with BPH than in healthy ones on Week +8 (PDogs affected with BPH were significantly older (Pdogsdogs; 75% of the cases responded with an improvement. The only minor and possibly treatment-related events observed were a short-lasting exacerbation of clinical signs of BPH (two dogs), increased weight gain (three dogs), and anxiety (three dogs) with one of these dogs developing a blunt coat. These results demonstrate the clinical efficacy and overall safety of the Gonazon implants.

  1. Brown Planthopper Resistance Genes in Rice: from Germplasm to Breeding

    Institute of Scientific and Technical Information of China (English)

    G.C. He

    2007-01-01

    @@ The brown planthopper (BPH), Nilaparvata lugens Stal (Homoptera: Delphacidae), is one of the most destructive and widespread insect pests of rice (Oryza sativa) that can be found throughout the rice-growing areas in Asia, causing significant yield loss in susceptible cultivars every year.

  2. Pharmacological researches of Syngnathus acus Linnaeus on mouse benign prostatic hyperplasia model induced by fetal urogenital sinus implants%尖海龙对胚胎尿生殖窦致小鼠前列腺增生模型的药理作用

    Institute of Scientific and Technical Information of China (English)

    许东晖; 蒋国津; 梅雪婷; 许实波

    2011-01-01

    目的 研究尖海龙对尿生殖窦致小鼠前列腺增生(benign prostatic hyperplasia,BPH)模型的药理作用,探讨尖海龙应用于治疗良性前列腺增生海洋药物开发的可行性.方法 建立16 d龄胎鼠尿生殖窦组织植入法诱导小鼠BPH模型,连续灌胃给予尖海龙(50,100,200 mg·kg-1)30 d后,评价对BPH小鼠前列腺指数和精囊腺指数、血清酸性磷酸酶(ACP)、前列腺组织形态参数的影响.结果 与模型组相比,尖海龙具有升高精囊腺指数,降低前列腺指数、血清ACP、腺体周长、腺体面积、腺上皮高度的作用.结论 尖海龙具有治疗BPH的作用,同时补肾壮阳,克服治疗前列腺增生药物阳痿少精等副作用,可开发成为治疗BPH的海洋药物.%Objective To study the effects of Syngnathus acus Linnaeus on mouse benign prostatic hyperplasia (BPH) induced by fetal urogenital sinus implants, and to investigate the possibility of applying Syngnathus acus Linnaeus to BPH treatment. Methods Mouse BPH model was established by implanting 16 days old embryo urogenital sinus of mouse. After oral administration of Syngnathus acus Linnaeus (50,100,200 mg · kg-1) for 30 days, the indices of seminal vesicle and prostatic weight, serum acid phosphatase (ACP), prostatic gland perimeter, gland area and gland epithelial height of mice were measured. Results Compared to BPH model group,oral administration of Syngnathus acus Linnaeus reduced the prostatic weight, serum ACP, prostatic gland perimeter, gland area and gland epithelial height, and increased the index of seminal vesicle. Conclusion As a traditional drug for invigorating the kidney and strengthening Yang, increasing number of sperms, Syngnathus acus Linnaeus showed therapeutic effects on BPH, hence could be developed a marine drug for treatment of BPH without side effects such as impotence and oligospermatism.

  3. Multiple factors related to detrusor overactivity in Chinese patients with benign prostate hyperplasia

    Institute of Scientific and Technical Information of China (English)

    LIU Ning; MAN Li-bo; HE Feng; HUANG Guang-lin; WANG Hai; LI Gui-zhong; WANG Jian-wei; L(U) Yan-wei

    2012-01-01

    Background Detrusor overactivity (DO) is a known cause of lower urinary tract symptoms and occurs in 50%-75% of benign prostate hyperplasia (BPH) patients.We sought to investigate the clinical and urodynamic factors that are associated with the presence of DO in Chinese BPH patients.Methods Two hundred and eighty-seven consecutive patients with clinical BPH were retrospectively evaluated in this study.Each patient underwent urodynamic evaluation and completed the International Prostate Symptom Score (IPSS)and Quality of Life (QoL) questionnaire.Patients with neurological symptoms or other diseases likely to affect detrusor functions were strictly excluded.The 184 BPH patients included in the study were divided into groups according to the presence of DO as shown in urodynamic tests.Univariate analysis of factors associated with the presence of DO were performed using Student's t-test and the Mann-Whitney test; multivariate analysis used stepwise Logistic regressions.The relationship between degree of bladder outlet obstruction (BOO) and DO was also investigated using a linear-by-linear association test.Results Of 184 BPH patients,DO was present in 76 (41.3%).On univariate analysis,patients with DO were older (P=0.000),and showed smaller maximal bladder capacity (MBC,P=0.000) and voided volume (P=0.000),higher maximal detrusor pressure (P=0.000) and projected isovolumetric pressure (PIP) (P=0.005),higher Abrams-Griffiths number (P=0.000) and degree of bladder outlet obstruction (P=0.000),higher IPSS (P=0.000) and irritative IPSS subscores (P=0.000).Stepwise Logistic regression analysis showed that PIP (OR=1.012,95% CI1.002-1.023,P=0.019),age (OR=1.030,95% CI 1.005-1.067,P=0.059),and MBC (OR=0.993,95% CI 0.990-0.996,P=0.000)were independent risk factors for DO in BPH patients.Linear-by-linear association tests indicated a positive linear association between DO and severity of BOO,with incidence of DO increasing with BOO grade (P=0.000).Conclusions In

  4. 良性前列腺增生患者健康相关生命质量的影响因素%Investigation of the health related quality of life in patients with benign prostatic hyperplusia

    Institute of Scientific and Technical Information of China (English)

    陈兰

    2009-01-01

    目的 探讨分析良性前列腺增生症(BPH)患者的健康相关生命质量(HRQOL)的影响因素,为有针对性的护理干预措施提供一些理论依据.方法 采用良性前列腺增生生活质量量表(BPHQLS)及国际前列腺症状评分表(IPSS),对100例BPH患者行问卷调查,对结果进行分析.结果 100例BPH患者IPSS评分轻度占16%,中度占32%,重度占52%;HRQOL总分平均为(278.16±29.42)分.BPH患者的HRQOL与疾病症状、年龄、婚姻状况呈负相关,而与文化程度无统计学意义,同时疾病症状对HRQOL的影响最直接、最明显.结论 疾病症状、年龄、婚姻状况对BPH患者的HRQOL有影响,改善BPH患者HRQOL的首要任务就是要有效地减轻其下尿路症状(LETS),综合考虑提出相应的护理干预措施,能更大程度地提高BPH患者的HRQOL.%Objective To evaluate the health-related quality of life(HRQOL)of patients with benign prostatic hyperplasia(BPH)and its related influential factors.Provide theory basis for nursing intervention.Methods A total of 100 BPH patients were investaged with HRQOL and International Prostate Symptom Scale (IPSS)and the results wer analyzed.Results The IPSS results of 100 patients were that the Mild occupied 16%,the Moderate occupied 32% and the Severe occupied 52%,and the total scores of HRQOL was(278.16±29.42).The HRQOL of BPH had negative correlation with symptoms,age and marital status,and there was no relation with Education level.THE symptom had The most obvious influence on HRQOL of patients with BPH.Conclusions H RQOL of patients with BPH was influenced by the symptom,age,and Marital status,so the first step to improve the HRQOL of patients with BPH was to relieve the lower urinary tract symptoms(LUTS)effectively.Consider all these mentioned aspects before providing corrosponding nursing measures can improve the HRQOL greatly.

  5. Smoking habits and benign prostatic hyperplasia: A systematic review and meta-analysis of observational studies.

    Science.gov (United States)

    Xu, Huan; Fu, Shi; Chen, Yanbo; Chen, Qi; Gu, Meng; Wang, Zhong

    2016-08-01

    Previous studies have warned against the promoting effects of cigarette smoking on benign prostatic hyperplasia (BPH). In contrast, some have argued that smoking confers a protective effect regarding BPH, while others have observed an aggravated effect. Thus, we performed this meta-analysis to determine whether cigarette use is associated with BPH risk.To identify articles from observational studies of relevance, a search was performed concurrent to March 21, 2016, on PubMed, Web of Science, Cochrane, EBSCO, and EMBASE databases. Random-effect model, according to the heterogeneity, was calculated to reveal the relative risks (RRs) and corresponding 95% confidence intervals (CIs).Eight articles were included in this meta-analysis, representing data for 44,100 subjects, of which 5221 (11.8%) had BPH as defined according to the criteria. Seven reports are concerned with analysis between nonsmokers and ex-smokers, in which no significant difference was observed (RR = 0.99, 95% CI 0.94-1.05). Another meta-analysis of 7 studies indicated an observable trend, but without significant difference between groups of nonsmokers and current smokers (RR = 1.17, 95% CI 0.98-1.41). Between groups of heavy (6 articles; RR = 1.02, 95% CI 0.84-1.24) and light smokers (5 articles; RR = 0.90, 95% CI 0.71-1.15), again no significant difference appears. Finally, we combined individuals as never-smokers and ever-smokers and still found no significant difference between the 2 groups of patients (RR = 1.03, 95% CI 0.92-1.15). Sensitivity analysis was displayed and confirmed the stability of the present results.Combined evidence from observational studies shows no significant association between cigarette smoking and BPH risk, either for ex-smokers or for current smokers. The trend of elevated BPH risk from smoking was observed only in current smokers compared with nonsmokers, while marginal significance was observed in comparing ever-smokers with never-smokers in

  6. Smoking habits and benign prostatic hyperplasia

    Science.gov (United States)

    Xu, Huan; Fu, Shi; Chen, Yanbo; Chen, Qi; Gu, Meng; Wang, Zhong

    2016-01-01

    Abstract Previous studies have warned against the promoting effects of cigarette smoking on benign prostatic hyperplasia (BPH). In contrast, some have argued that smoking confers a protective effect regarding BPH, while others have observed an aggravated effect. Thus, we performed this meta-analysis to determine whether cigarette use is associated with BPH risk. To identify articles from observational studies of relevance, a search was performed concurrent to March 21, 2016, on PubMed, Web of Science, Cochrane, EBSCO, and EMBASE databases. Random-effect model, according to the heterogeneity, was calculated to reveal the relative risks (RRs) and corresponding 95% confidence intervals (CIs). Eight articles were included in this meta-analysis, representing data for 44,100 subjects, of which 5221 (11.8%) had BPH as defined according to the criteria. Seven reports are concerned with analysis between nonsmokers and ex-smokers, in which no significant difference was observed (RR = 0.99, 95% CI 0.94–1.05). Another meta-analysis of 7 studies indicated an observable trend, but without significant difference between groups of nonsmokers and current smokers (RR = 1.17, 95% CI 0.98–1.41). Between groups of heavy (6 articles; RR = 1.02, 95% CI 0.84–1.24) and light smokers (5 articles; RR = 0.90, 95% CI 0.71–1.15), again no significant difference appears. Finally, we combined individuals as never-smokers and ever-smokers and still found no significant difference between the 2 groups of patients (RR = 1.03, 95% CI 0.92–1.15). Sensitivity analysis was displayed and confirmed the stability of the present results. Combined evidence from observational studies shows no significant association between cigarette smoking and BPH risk, either for ex-smokers or for current smokers. The trend of elevated BPH risk from smoking was observed only in current smokers compared with nonsmokers, while marginal significance was observed in comparing ever-smokers with

  7. 良性前列腺增生症并发前列腺结核%Benign Prostatic Hyperplasia with tuberculosis

    Institute of Scientific and Technical Information of China (English)

    姜睿; 陈明; 李洪位; 陈同良

    2001-01-01

    Purpose:To explore the clinical features of benign prostate hypertrophy (BPH) with tuberculosis.Method:Retrospective analysis of 8 cases BPH with tuberculosis has been made.Results:6 cases underwent open surgery and 1 case was treated with antituberculosis therapy,and 1 cases was treated by TURP.6 cases were followed up for an average of 10.8 months.The therapeutic results were satisfactory.Conclusions:Clinically, BPH with tuberculosis was often mistaken for BPH or prostatic carcinoma, so attention should be paid to the differential diagnosis. The final diagnosis should rely on pathological examination.%目的:提高对良性前列腺增生症(BPH)并发前列腺结核的认识。方法:回顾性分析8例BPH并发前列腺结核患者临床资料。结果:8例BPH并发前列腺结核均依靠病理检查确立诊断。1例接受抗结核治疗,1例接受TURP,6例接受开放手术。1例发生切口延迟愈合和尿瘘,另6例平均随访10.8个月,疗效满意。结论:BPH并发前列腺结核极易与 BPH或前列腺癌混淆, 确诊依靠病理检查,一经确诊,应及时应用抗结核药物,防止并发症。

  8. Examination of CK2α and NF-κB p65 expression in human benign prostatic hyperplasia and prostate cancer tissues.

    Science.gov (United States)

    Qaiser, Fatima; Trembley, Janeen H; Sadiq, Sarah; Muhammad, Iqbal; Younis, Rubina; Hashmi, Shoaib Naiyar; Murtaza, Badar; Rector, Thomas S; Naveed, Abdul Khaliq; Ahmed, Khalil

    2016-09-01

    Protein kinase CK2 plays a critical role in cell growth, proliferation, and suppression of cell death. CK2 is overexpressed, especially in the nuclear compartment, in the majority of cancers, including prostate cancer (PCa). CK2-mediated activation of transcription factor nuclear factor kappa B (NF-κB) p65 is a key step in cellular proliferation, resulting in translocation of NF-κB p65 from the cytoplasm to the nucleus. As CK2 expression and activity are also elevated in benign prostatic hyperplasia (BPH), we sought to increase the knowledge of CK2 function in benign and malignant prostate by examination of the relationships between nuclear CK2 and nuclear NF-κB p65 protein expression. The expression level and localization of CK2α and NF-κB p65 proteins in PCa and BPH tissue specimens was determined. Nuclear CK2α and NF-κB p65 protein levels are significantly higher in PCa compared with BPH, and these proteins are positively correlated with each other in both diseases. Nuclear NF-κB p65 levels correlated with Ki-67 or with cytoplasmic NF-κB p65 expression in BPH, but not in PCa. The findings provide information that combined analysis of CK2α and NF-κB p65 expression in prostate specimens relates to the disease status. Increased nuclear NF-κB p65 expression levels in PCa specifically related to nuclear CK2α levels, indicating a possible CK2-dependent relationship in malignancy. In contrast, nuclear NF-κB p65 protein levels related to both Ki-67 and cytoplasmic NF-κB p65 levels exclusively in BPH, suggesting a potential separate impact for NF-κB p65 function in proliferation for benign disease as opposed to malignant disease.

  9. Role of IAPs in prostate cancer progression: immunohistochemical study in normal and pathological (benign hyperplastic, prostatic intraepithelial neoplasia and cancer human prostate

    Directory of Open Access Journals (Sweden)

    Olmedilla Gabriel

    2010-01-01

    Full Text Available Abstract Background In this study was investigate IAPs in normal human prostate (NP, benign prostatic hyperplasia (BPH, prostatic intraepithelial neoplasia (PIN and prostatic carcinoma (PC, and their involvement in apoptosis/proliferation via NF-kB (TNF-α, IL-1 stimulation. Methods Immunohistochemical and Western blot analyses were performed in 10 samples of normal prostates, 35 samples of BPH, 27 samples diagnosis of PIN (with low-grade PIN or high-grade PIN and 95 samples of PC (with low, medium or high Gleason grades. Results In NP, cytoplasm of epithelial cells were positive to c-IAP1/2 (80% of samples, c-IAP-2 (60%, ILP (20%, XIAP (20%; negative to NAIP and survivin. In BPH, epithelial cells were immunostained to c-IAP1/2 (57.57%, c-IAP-2 (57.57%, ILP (66.6%, NAIP (60.6%, XIAP (27.27%, survivin (9.1%. Whereas low-grade PIN showed intermediate results between NP and BPH; results in high-grade PIN were similar to those found in PC. In PC, epithelial cells were immunostained to c-IAP1/2, c-IAP-2, ILP, NAIP, XIAP (no Gleason variation and survivin (increasing with Gleason. Conclusions IAPs could be involved in prostate disorder (BPH, PIN and PC development since might be provoke inhibition of apoptosis and subsequently cell proliferation. At the same time, different transduction pathway such as IL-1/NIK/NF-kB or TNF/NF-kB (NIK or p38 also promotes proliferation. Inhibitions of IAPs, IL-1α and TNFα might be a possible target for PC treatment since IAPs are the proteins that inhibited apoptosis (favour proliferation and IL-1α and TNFα would affect all the transduction pathway involucrate in the activation of transcription factors related to survival or proliferation (NF-kB, Elk-1 or ATF-2.

  10. Expression of kallikrein-related peptidase 7 is decreased in prostate cancer

    Directory of Open Access Journals (Sweden)

    Chong-Yu Zhang

    2015-02-01

    Full Text Available Recent evidence suggests that the human kallikrein 7 (KLK7 is differentially regulated in a variety of tumors. The aim of this study was to determine the expression of kallikrein-related peptidase 7 and KLK7 in our large collection of prostate samples. Between August 2000 and December 2012, 116 patients with histologically confirmed prostate cancer (PCa and 92 with benign prostate hyperplasia (BPH were recruited into the study. Using immunohistochemistry, quantitative reverse transcription polymerase chain reaction (RT-PCR and western blot, kallikrein-related peptidase 7 expression in BPH and PCa tissues was determined at the mRNA and protein levels. The relationships between kallikrein-related peptidase 7 mRNA expression and clinicopathological features were analyzed. A total of 64 of 92 (69.57% benign cases showed positive staining for KLK7 and 23 of 116 (19.83% malignant cases showed positive, the difference of KLK7 expression between PCa and BPH was statistically significant (P < 0.001. The expression level of kallikrein-related peptidase 7 mRNA was significantly decreased in PCa tissues compared with that in BPH tissues and normal prostate tissue. Kallikrein-related peptidase 7 mRNA exhibited different expression patterns in terms of localization depending on pathological category of PCa. Similarly, our western immunoblot analyses demonstrated that the protein expression levels of KLK7 was lower in PCa than in BPH tissues and normal prostate tissue. Kallikrein-related peptidase 7 and KLK7 expression are down-regulated in PCa and lower expression of kallikrein-related peptidase 7 closely correlates with higher Gleason score and higher prostate-specific antigen level.

  11. Is there any relationship between PSA and increased peripheral CD4+CD25highFOX3+ Treg in prostate cancer patients?

    Directory of Open Access Journals (Sweden)

    Yigit Akin

    2011-09-01

    Full Text Available Introduction: The aims of this study were fi rst, to determine whether peripheral levels of CD4+CD25highFoxp3+ regulatory T cells (Treg are elevated in Prostate Cancer (PCa patients, and second, to determine the directcorrelation between peripheral Treg and total serum Prostate Specifi c Antigen (PSA levels in these patients.Methods: Peripheral Blood Mononuclear Cells from 56 subjects undergoing diagnostic prostate biopsies (PSA ≥ 2.5 ng/ml were analyzed for Treg numbers. The association between the peripheral Treg and serum PSA values was fi rst determined in the entire population, including people with no prostate pathology and PCa and Benign Prostate Hyperplasia (BPH patients, and second, in nine PCa patients before and after curative prostatectomy.Results: This project was performed in Akdeniz University immunology laboratory and urology out patient clinic from 2008 to 2010. Peripheral Treg frequencies were signifi cantly increased in PCa patients (n = 19, 3.23 ± 1.59 compared with BPH patients (n = 27, 1.66 ± 0.80 and healthy subjects (n = 10, 1.08 ± 0.43 (p < 0.01. The percentage of Treg in BPH patients was also signifi cantly higher than that of healthy subjects (p < 0.01. Importantly, the increase in BPH and PCa patients paralleled the elevation in total serum PSA levels, demonstrating a strong positive correlation (r = 0.75; p < 0.01.Conclusion: These results demonstrate that peripheral Treg densities are correlated with PSA in BPH and PCa patients, suggesting that PSA may have a role in Treg induction and/or maintenance in Treg in these people.

  12. Expression of kallikrein-related peptidase 7 is decreased in prostate cancer

    Institute of Scientific and Technical Information of China (English)

    Chong-Yu Zhang; Yu Zhu; Wen-Bin Rui; Jun Dai; Zhou-Jun Shen

    2015-01-01

    Recent evidence suggests that the human kallikrein 7 (KLK7) is differentially regulated in a variety of tumors. The aim of this study was to determine the expression of kallikrein‑related peptidase 7 and KLK7 in our large collection of prostate samples. Between August 2000 and December 2012, 116 patients with histologically confirmed prostate cancer (PCa) and 92 with benign prostate hyperplasia (BPH) were recruited into the study. Using immunohistochemistry, quantitative reverse transcription polymerase chain reaction (RT‑PCR) and western blot, kallikrein‑related peptidase 7 expression in BPH and PCa tissues was determined at the mRNA and protein levels. The relationships between kallikrein‑related peptidase 7 mRNA expression and clinicopathological features were analyzed. A total of 64 of 92 (69.57%) benign cases showed positive staining for KLK7 and 23 of 116 (19.83%) malignant cases showed positive, the difference of KLK7 expression between PCa and BPH was statistically significant (P < 0.001). The expression level of kallikrein‑related peptidase 7 mRNA was significantly decreased in PCa tissues compared with that in BPH tissues and normal prostate tissue. Kallikrein‑related peptidase 7 mRNA exhibited different expression patterns in terms of localization depending on pathological category of PCa. Similarly, our western immunoblot analyses demonstrated that the protein expression levels of KLK7 was lower in PCa than in BPH tissues and normal prostate tissue. Kallikrein‑related peptidase 7 and KLK7 expression are down‑regulated in PCa and lower expression of kallikrein‑related peptidase 7 closely correlates with higher Gleason score and higher prostate‑specific antigen level.

  13. ZFP91-a newly described gene potentially involved in prostate pathology.

    Science.gov (United States)

    Paschke, Lukasz; Rucinski, Marcin; Ziolkowska, Agnieszka; Zemleduch, Tomasz; Malendowicz, Witold; Kwias, Zbigniew; Malendowicz, Ludwik K

    2014-04-01

    In search for novel molecular targets in benign prostate hyperplasia (BPH), a PCR Array based screening of 84 genes was performed. Of those, expression of ZFP91 (ZFP91 zinc finger protein) was notably upregulated. Limited data concerning the function of ZFP91 product show that it is a potential transcription factor upregulated in human acute myelogenous leukemia and most recently found to be the non-canonical NF-κB pathway regulator. In order to test this finding on a larger number of samples, prostate specimens were obtained from patients undergoing adenomectomy for BPH (n = 21), and as a control, from patients undergoing radical cystectomy for bladder cancer (prostates unchanged pathologically, n = 18). Similar studies were performed on cultured human prostate cancer cell lines: LNCaP, DU145, 22Rv1, PC-3; as well as normal prostate epithelial cells-PrEC. Methods employed included: Human Obesity PCR Array (Qiagen), QPCR and Western blotting. QPCR studies confirmed significant overexpression of ZFP91 in BPH samples. On a protein level, however, comparison between normal and BPH prostates revealed insignificant differences. As for prostate cell lines examined, all expressed ZFP91 mRNA. Western blotting analysis showed markedly higher protein levels of ZFP91 in all cancer cell lines in comparison with normal (PrEC) cells. In conclusion, the upregulated ZFP91 mRNA in BPH, not accompanied by parallel changes in ZFP91 protein levels, together with ZFP91 protein abundance in prostate cancer cell lines suggest ZFP91 involvement in these prostate diseases.

  14. Human Papilloma Virus Detection by INNOLiPA HPV in Prostate Tissue from Men of Northeast Mexico

    Science.gov (United States)

    Dávila-Rodríguez, Martha I; Ignacio Morales, Cesar V; Aragón Tovar, Anel R; Olache Jimenez, Delia; Castelán Maldonado, Edmundo; Lara Miranda, Sandra; Cortés Gutiérrez, Elva I

    2016-11-01

    Background: Prostatic adenocarcinoma by Prosate cancer (PCa) is the most prevalent cancer and the second cause of cancer-related death among men in the Western world. Human papilloma virus (HPV) may be considered as a preventable risk factor. In this study, we assessed the frequencies of HPV infection in prostatic adenocarcinoma and benign prostatic hyperplasia (BPH) cases in Northeast Mexico. Materials and Methods: A total of 87 paraffin-embedded blocks (from 25 and 62 patients with definite diagnoses of BPH and adenocarcinoma, respectively) were selected and subjected to INNOLiPA HPV Genotyping to detect 28 high- and low-risk HPV types. The rates of infection were compared in the two studied groups. Results: INNOLiPA HPV demonstrated great sensitivity for HPV detection on paraffin-embedded tissue. Global prevalence was 14.9% (13/87). HPV infection was positive in 19.4% (12/62) of patients with adenocarcinoma and 4.0% (1/25) of patients with BPH. HPV-11, which is considered to be low risk, was more prevalent. Interestingly, one patient with BPH and six with prostate cancer showed examples considered to be high risk (HPV-18, -51, -52, and -66). Conclusion: A higher rate of HPV infection among Mexican patients with prostatic carcinoma than among those with BPH was observed. HPV infections may thus contribute to the risk of prostate cancer. Further studies are required to elucidate any roles of HPV infection in prostate disease in Mexico and the effect of prevention and treatment of HPV infection on prostatic adenocarcinoma.

  15. Preliminary assessment of neck circumference in benign prostatic hyperplasia in patients with metabolic syndrome

    Science.gov (United States)

    Akin, Yigit; Gulmez, Hakan; Ates, Erhan; Gulum, Mehmet; Savas, Murat

    2017-01-01

    ABSTRACT Objectives To investigate the impact of neck circumference (NC) in the treatment of bening prostatic hyperplasia (BPH) patients with metabolic syndrome (MtS). Additionally, we determined dose response to alpha-blockers and cut-off values for NC and waist circumference (WC), in these patients. Materials and Methods Non-randomized, open-labelled, and multi-centre study was conducted between March 2014 and September 2015. The BPH patients were enrolled and were divided into 2 groups: with MtS (Group 1; n=94) and without MtS (Group 2; n=103). Demographic data, anthropometric measurements, blood analyses, uroflowmetric parameters, post voiding residual urine (PVR), prostate volume, quality of life (QoL) index, NC and WC were recorded. Both groups were administered oral alpha-blockers and response to treatment was evaluated. Receiver-operating characteristic (ROC) curves were obtained and significant p was p<0.05 . Results In total, 197 patients were enrolled with mean age of 60.5±8.1 years. Mean NC and WC were higher in MtS patients (p<0.001). Uroflowmetry parameters and QoL indexes were comparable between groups before treatment. International prostate symptom score, uroflowmetry parameters, and QoL significant improved in Group 2 than Group 1, at 1 st and 6 th months of treatment with alpha-blockers. Success rate of treatment was significant higher in Group 2 than Group 1 (p<0.001). Cut-off values were 42.5cm and 113.5cm for NC and WC respectively, for response to alpha-blockers in BPH patients with MtS. Conclusions MtS can be related with BPH and can negatively affect the response to alpha-blocker treatment. NC can be used for predicting response to alpha-blocker treatment in BPH patients with MtS.

  16. 1H-MRSI of prostate cancer: The relationship between metabolite ratio and tumor proliferation

    Energy Technology Data Exchange (ETDEWEB)

    Wang Xizhen [Shandong Medical Imaging Research Institute, Shandong University, 324 JingWu Road, Jinan 250021 (China) and Department of Radiology, Medical Imaging Center of the Affiliated Hospital, Weifang Medical University, 288 Shengli East Street, Weifang 261042 (China)], E-mail: zhen94320@yahoo.com.cn; Wang Bin [Department of Radiology, Medical Imaging Center of the Affiliated Hospital, Weifang Medical University, 288 Shengli East Street, Weifang 261042 (China)], E-mail: binwang001@yahoo.com.cn; Gao Zhiqin [Department of Medical Biology, Weifang Medical University, 288 Shengli East Street, Weifang 261042 (China)], E-mail: zhiqin_gao@yahoo.com.cn; Liu Jingang [Department of Radiology, Medical Imaging Center of the Affiliated Hospital, Weifang Medical University, 288 Shengli East Street, Weifang 261042 (China)], E-mail: liujingang77@163.com; Liu Zuoqin [Shandong Medical Imaging Research Institute, Shandong University, 324 JingWu Road, Jinan 250021 (China)], E-mail: liuzuoqin666@yahoo.com.cn; Niu Qingliang [Department of MRI/CT, Weifang Traditional Chinese Medicine Hospital, Weifang 261041 (China)], E-mail: qingliangniu@yahoo.com.cn; Sun Zhenkui [Department of Radiology, Medical Imaging Center of the Affiliated Hospital, Weifang Medical University, 288 Shengli East Street, Weifang 261042 (China)], E-mail: sun77126@163.com; Yuan Yuxiao [Department of Radiology, Medical Imaging Center of the Affiliated Hospital, Weifang Medical University, 288 Shengli East Street, Weifang 261042 (China)], E-mail: realyyx@eyou.com

    2010-02-15

    Purpose: To investigate whether 1H-MRSI can be used to predict the proliferative activity of prostate cancer. Materials and methods: Thirty-eight patients with prostate cancer (PCa) and thirty-three patients with benign prostate hyperplasia (BPH) were included in this study. Patients were examined in supine position using a 1.5 T superconducting magnetic scanner equipped with a pelvic phased-array multi-coil and CSI-3D-PROSTATE sequence. Commercial software was used to acquire and process MR spectroscopic imaging data. Mean (Cho + Cr)/Cit ratios of PCa, BPH, and peripheral zone (PZ) were calculated. Cellularity of PCa was recorded based on hematoxylin and eosin staining. PCNA was detected using immunohistochemical techniques. Results: The mean (Cho + Cr)/Cit ratio of the peripheral zone (0.38 {+-} 0.09) was lower than that of BPH (0.51 {+-} 0.19) (P < 0.05). The average value of (Cho + Cr)/Cit ratio of prostate cancer was 3.98 {+-} 0.12. The (Cho + Cr)/Cit ratio of prostate cancer was higher than that of the peripheral zone and BPH (P < 0.05). The cellularity and PCNA LI of prostate cancer were 12.90 {+-} 4.07% and 72.1 {+-} 19.01%, respectively. The (Cho + Cr)/Cit ratio of prostate cancer positively correlated with tumor cellularity (r = 0.582, P = 0.027) and PCNA LI (r = 0.495, P = 0.022). Conclusion: The (Cho + Cr)/Cit ratio of PCa can reveal the differences in proliferative activity between PCa and BPH. MRSIs are therefore able to predict the proliferative rate of variously differentiated prostate cancers.

  17. The remote effects of intravitreal anti-VEGF therapy.

    Science.gov (United States)

    Balta, F; Merticariu, M; Taban, C; Neculau, G; Merticariu, A; Muresanu, D; Badescu, D; Jinga, V

    2016-01-01

    Objective: To study the effects of intravitreal anti-Vascular Endothelial Growth Factor (VEGF) therapy with Avastin for wet Age-Related Macular Degeneration (AMD) on Benign Prostatic Hyperplasia (BPH)-related symptoms. Methods: An exploratory trial was conducted from August 1, 2013 to February 1, 2014, that included 14 male patients previously diagnosed with BPH, who were aged between 59 and 69 years. The trial was performed in Bucharest and involved two medical institutions: the Clinical Hospital of Eye Emergencies and the "Prof. Dr. Theodor Burghele" Hospital. This prospective study utilized both objective and subjective indicators to analyze the link between intravitreal anti-VEGF therapy for wet AMD and BPH. The evaluations consisted of uroflowmetry and International Prostate Symptom Score (I-PSS) assessments. Results: The maximum flow rate (Qmax) improved by an average of 5.05 ml/ sec in 9 patients, whereas the remaining 5 patients showed a slight decrease in Qmax (mean 1.6 ml/ sec). The I-PSS score improved, with an overall decrease of 1.18 points at follow-up compared to the initial score (mean initial score = 2.42; mean follow-up score = 1.24). Conclusion: The analysis revealed that anti-VEGF therapy for wet AMD had a significant positive effect on all BPH-related symptoms; patients reported improved urinary streams and decreased nocturia. Abbreviations: BPH = benign prostatic hyperplasia, AMD = age-related macular degeneration, VEGF = vascular endothelial growth factor, I-PSS = international prostate symptom score, Qmax = maximum flow rate, TSP-1 = thrombospondin-1, FGF-2 = fibroblast growth factor, mRNA = precursor messenger ribonucleic acid, PSA = prostate-specific antigen, DRE = digital rectal examination, AUR = acute urinary retention, COX2 = cyclooxygenase 2, QoL = quality of life.

  18. Relief of BPO or improvement in quality of life?

    Science.gov (United States)

    Teillac, P

    1998-01-01

    Benign prostatic hyperplasia (BPH) can cause benign prostatic enlargement with subsequent benign prostatic obstruction (BPO) and lower urinary tract symptoms (LUTS). A reduction in the size of the prostate has long been considered one of the most important treatment goals. However, there is a poor correlation between prostate size and both LUTS and BPO, and between BPO and symptoms. Today, the urologist's primary objectives are to minimize symptoms, relieve BPO and decrease the morbidity associated with BPO. From the patient's point of view, rapid relief of LUTS and immediate improvement in associated quality of life (QOL) are the most important factors. Although there is a good correlation between relief of symptoms (as measured by the International Prostate Symptom Score [I-PSS], for example) and associated improvement in bothersomeness and QOL, particularly that associated with filling ('irritative') symptoms, it is still important to quantify LUTS-related bothersomeness and QOL. Various questionnaires have been developed to measure bothersomeness (e.g. Symptom Problem Index [SPI], Danish PSS [DAN-PSS], International Continence Society BPH Study Group [ICSmale] questionnaire) and QOL (e.g. I-PSS-QOL, BPH Impact Index [BII] and QOL9 BPH-specific questionnaire). In addition, the impact of treatment on sexual function should also be taken into account when judging the overall well being or QOL of the patient. A grading system to evaluate the global improvement in patients following treatment has been established. Patients are either graded as showing 'slight', 'moderate' or 'marked' improvement, with the reduction in I-PSS or BII scores required for each classification dependent on baseline symptom severity. Medical treatment strategies designed to alleviate the symptoms of BPH and consequently improve the patient's QOL are now becoming increasingly important.

  19. Photoselective vaporization of the prostate in men with a history of chronic oral anti-coagulation

    Directory of Open Access Journals (Sweden)

    Omer F. Karatas

    2010-04-01

    Full Text Available PURPOSE: A considerable percentage of patients with benign prostatic hyperplasia (BPH also have additional cardiac pathologies, which often require anticoagulant therapy. The aim of this study was to evaluate the efficacy and safety of photoselective vaporization of the prostate (PVP for BPH in cardiac patients receiving anticoagulant therapy. MATERIALS AND METHODS: A total of 67 patients suffering from BPH and high risk cardiac pathologies were operated on using laser prostatectomy. All patients had cardiac pathologies with bleeding disorders requiring anticoagulant use, and underwent standard urologic evaluation for BPH. Patients were treated with laser prostatectomy for relief of the obstruction using the KTP/532 laser energy at 80 W. RESULTS: The mean patient age was 71.4 years (range 55-80. Mean prostate volume on transrectal ultrasonography was 73.2 mL (range 44-120. Operation time ranged from 40 to 90 min, with an average value of 55 min. The average hospital stay was 48 hours (range 12-72 and the Foley catheters were removed within 48 hours, with a mean catheterization time of 34.2 ± 5.9 hours (0-48. No patient required an additional procedure due to severe bleeding necessitating intervention during the early postoperative phase. Mean International symptoms scoring system (IPSS values and post voiding residual volume decreased and peak urinary flow rate increased (p < 0.001. Our results showed that the mean prostate volume had decreased by 53% at 6 months. CONCLUSIONS: High-power photo selective laser vaporization prostatectomy is a feasible, safe, and effective alternative for the minimal invasive management of BPH, particularly in cardiac patients receiving anticoagulant therapy.

  20. 经尿道前列腺电切术治疗高危良性前列腺增生疗效观察%Clinic observation on transurethral resection of prostate in the treatment of high-risk benign prostatic hypertrophy

    Institute of Scientific and Technical Information of China (English)

    许铭杨; 唐志忠

    2011-01-01

    Objective: To observe the clinical effect and safety on transurethral resection of prostate in the treatment of high-risk benign prostatic hypertrophy. Methods: 96 cases of high-risk BPH patients were given TURP, the observation of international prostate symptom score (IPSS) and maximal flow rate (MFR) were compared before and after operation. Results: The TURP was safe for 90 cases of high-risk BPH patients, there was no bemorrhea, transurethral resection syndromes (TURS) and death during the operation. Patients all passed perioperative period smoothly. Conclusion:The TURP is "the golden standard" in the treatment of BPH, we should deal with perioperative period with carefulness,concrete and individuation during the preoperation, intraoperation and postoperation to make the surgery risk of highrisk BPH down to the lower, and have a satisfactory effect.%目的:观察经尿道前列腺电切术(TURP)治疗高危良性前列腺增生(BPH)的手术疗效及安全性.方法:对96例高危BPH患者行TURP,观察比较术前、术后患者的症状评分(IPSS)和最大尿流量(MFR).结果:96例行经尿道前列腺电切术的高危BPH患者全部耐受手术,术中未发生大出血及电切综合征(transurethral reseetion syndromes,TURS),无死亡,均顺利度过围手术期.结论:TURP作为治疗BPH的"金标准",在细致、具体、个体化的做好术前、术中、术后的围术期处理的前提下,可将高危BPH手术的风险降至最低,取得满意疗效.

  1. Reversal of benign prostate hyperplasia by selective occlusion of impaired venous drainage in the male reproductive system: novel mechanism, new treatment.

    Science.gov (United States)

    Gat, Y; Gornish, M; Heiblum, M; Joshua, S

    2008-10-01

    The prostate is an androgen-regulated exocrine gland producing over 30% of the noncellular components of the semen and promoting optimal conditions for survival and motility of sperm in the vagina. Benign prostate hyperplasia (BPH) is the most common benign neoplasm in men. Its aetiology is not clear, and therefore, current medical treatments are directed towards the symptoms. Though testosterone is known to be the promoter of prostate cell proliferation, no causal relation between serum testosterone levels and BPH has been found. In this study, we propose a novel and tested pathophysiological mechanism for the evolution of BPH and suggest a tested and effective treatment. We found that in all BPH patients, the one-way valves in the vertically oriented internal spermatic veins are destroyed (clinically manifested as varicocele), causing elevated hydrostatic pressure, some 6-fold greater than normal, in the venous drainage of the male reproductive system. The elevated pressure propagates to all interconnected vessels leading to a unique biological phenomenon: venous blood flows retrograde from the higher pressure in the testicular venous drainage system to the low pressure in the prostatic drainage system directly to the prostate (law of communicating vessels). We have found that free testosterone levels in this blood are markedly elevated, with a concentration of some 130-fold above serum level. Consequently, the prostate is exposed to: (i) increased venous pressure that causes hypertrophy; (ii) elevated concentration of free testosterone causing hyperplasia. We have treated 28 BPH patients using a technique that restores normal pressure in the venous drainage in the male reproductive system. The back-pressure and the back-flow of blood from the testicular to the prostate drainage system were eliminated and, consequently, a rapid reduction in prostate volume and a regression of prostate symptoms took place.

  2. Clinical effects and economical impact of dutasteride and finasteride therapy in Italian men with LUTS

    Directory of Open Access Journals (Sweden)

    Luca Cindolo

    2013-12-01

    Full Text Available Objectives: To investigate differences in the risk of benign prostatic hyperplasia (BPH- related hospitalization, for surgical and non-surgical reasons, and of new prostate cancer (PCa diagnosis between patients under dutasteride or finasteride treatment. Material and methods: A retrospective cohort study was conducted using data from record-linkage of administrative databases. Men aged ≥ 40 years old who had received a prescription for at least 10 boxes/year (index years: 2004-06 were included. The association of the outcomes was assessed using a multiple Cox proportional hazard model. Propensity scorematched analysis and a 5-to-1, greedy 1:1 matching algorithm were performed. The budget impact analysis of dutasteride vs finasteride in BPH-treated patient was performed. Results: From an initial cohort of about 1.5 million of Italian men, 19620 were selected. The overall hospitalization for BPH-non surgical reasons, for BPH-related surgery and for new detection of PCa incidence rates (IRs were 8.20 (95% CI, 7.62-8.23, 18.0 (95% CI, 17.12-18.93 and 8.62 (95% CI, 8.03-9.26 per 1000 person-years, respectively. The multivariate analysis after the propensity score-matching showed that dutasteride was associated with an independent reduced likelihood of hospitalization for BPH-related surgery (HR 0.82; 95% CI 0.73-0.93; p = 0.0025 and of newly detected PCa (HR: 0.76,95% CI, 0.65-0.85; p = 0.0116. The IR for BPH-non surgical reasons was 8.07 (95% CI, 7.10-9.17 and 9.25 (95% CI, 8.19-10.44 per 1000 person-years, respectively. The IR for BPH-related surgery was 18.28 (95% CI, 17.17-20.32 and 21.28 (95% CI, 19.24-23.06 per 1000 person-years among patients under dutasteride compared with those under finasteride, respectively. For new-onset PCa, the IR was 8.01 (95% CI, 7.07-9.08 and 9.38 (95% CI, 8.32-10.58 per 1000 person-years The pharmacoeconomical evaluation showed that the net budget impact of the use of dutasteride vs. finasteride in 1000 BPH

  3. 总前列腺特异性抗原及游离前列腺特异性抗原/总前列腺特异性抗原对高龄老年人前列腺癌的诊断与鉴别诊断%T-PSA and f-PSA/t-PSA in the diagnosis of prostate cancer in the elders

    Institute of Scientific and Technical Information of China (English)

    刘欣; 张舜欣; 林倩; 唐杰

    2011-01-01

    Objective To evaluate the application of t-PSA and f-PSA/t-PSA in the diagnosis and differential diagnosis of prostate cancer.Methods T-PSA and f-PSA of patients over 80 years old who were diagnosed of BPH or PCA pathologically by transrectal ultrasound guided biopsies were analyzed retrospectively.Results The ratio of patients with t-PSA≥4.0 μg/L is 85.5% ( BPH 40.6% , PCA 41.9% ,others 3.0%) , and t-PSA≥10.0 μg/L is 59.4% ( BPH 23.9% , PCA 32.9%,others 2.6%).Among the paiients with t-PSA<4.0 μg/L, there are 26 BPH and 6 PCA, and the average f-PSA/t-PSA of each is 0.26±0.14 and 0.24±0.13.Among 4.0≤t-PSA<10.0 μg/L, 39 BPH and 21 PCA, and the average f-PSA/t-PSA of each is 0.26±0.09 and 0.11±0.06, respectively.Among t-PSA≥10.0 μg/L, 56 BPH and 77 PCA, and the average f-PSA/t-PSA of each is 0.25±0.07 and 0.13±0.05, respectively.Conclusion For the elder patients, there is no statistical significance to use t-PSA ≥4.0 μg/L or t-PSA≥10.0 μg/L for the diagnosis or differential diagnosis of BPH and PCA.For the patients with t-PSA≥10.0 μg/L and 4.0≤t-PSA<10.0 μg/L, there is statistical significance for f-PSA/t-PSA between BPH and PCA.I%目的 探讨总前列腺特异性抗原(t-PSA)及游离前列腺特异性抗原(f-PSA)/t-PSA比值在高龄老年人(≥80岁)中的表现,并评价其在前列腺癌(PCA)和前列腺增生(BPH)鉴别诊断中的作用.方法 对经直肠超声引导下前列腺穿刺活检诊断为BPH和PCA的高龄患者234例,结合其术前的PSA检测结果,进行回顾性分析和统计.结果 t-PSA≥4.0 μg/L者高达85.5%(BPH 40.6%,PCA 41.9%,其他3.0%),而t-PSA≥10.0 μg/L者达59.4%(BPH 23.9%,PCA32.9%,其他2.6%).在t-PSA<4.0 μg/L的患者中,BPH 26例,平均f-PSA/t-PSA值0.26±0.14;PCA 6例,平均f-PSA/t-PSA值0.24±0.13.4.0≤t-PSA<10.0 μg/L的患者中,BPH 39例,平均f-PSA/t-PSA值0.26±0.09;PCA 21例,平均f-PSA/t-PSA值0.11±0.06.t-PSA≥10.0 μg/L的患者中,BPH 56

  4. Effect of Yishentong Capsules on Prostate Histological Changes in Mice with Prostatitis and Prostate Hyperplasia%益肾通对前列腺炎及前列腺增生组织学的影响

    Institute of Scientific and Technical Information of China (English)

    邱云桥; 祝常德; 李信平; 孟磊; 魏宗彬; 王志刚; 王小俊; 陈铭

    2013-01-01

    Objective To observe the effect of Yishentong Capsules(YC)on the histological changes of prostate gland in prostatitis and prostate hyperplasia.Methods Seventy male KM mice were randomly divided into normal control group,chronic nonbacterial prostatitis group(CNP),benign prostatic hyperplasia group(BPH),prostatitis with prostatic hyperplasia group(CNP+BPH),prostatitis YC intervention group(CNP-YC),benign prostatic hyperplasia YC intervention group(BPH-YC),prostatitis with benign prostatic hyperplasia YC intervention group(CNP+ BPH-YC).Except for the normal control group,mice in the other groups were modeled in accordance with the corresponding modeling method.The intervention groups were treated with YC at 1.0 g/kg by intragastric administration,and the same volume of distilled water was given to the mice in normal control group and model groups.On day 28,all of the mice were executed,the pathological changes of prostate gland were observed,and prostate IL-8 and Ki67 expression levels were also detected by immunohistochemical assay.Results The Ki67-LI in CNP+BPH group(73.20 ± 5.94)was higher than that in BPH group(51.20 ±4.20),and the IL-8-LI in CNP-YC group(13.90±1.91)was lower than that in CNP group(44.10 ± 3.67),the difference being significant(P<0.01).CNP+BPH-YC group had lower IL-8-LI level(15.90 ± 2.56)than CNP+BPH group(54.20 ± 3.29),the difference being significant(P<0.01).Conclusion Yishentong Capsules can reduce the incidence of benign prostatic hyperplasia by relieving the inflammatory changes in prostate gland.%目的 观察益肾通胶囊(YST)对小鼠前列腺炎及前列腺增生组织学变化的影响.方法 雄性KM小鼠随机分为7组,即正常对照组(Normal)、前列腺炎组(CNP)、前列腺增生组(BPH)、前列腺炎合并前列腺增生组(CNP+BPH)、前列腺炎干预组(CNP-YST)、前列腺增生干预组(BPH-YST)、前列腺炎合并前列腺增生干预组(CNP+BPH-YST).除正常对照组外,其余各组动物分别按照

  5. 人良性前列腺增生组织中基底细胞去分化与雌激素受体α表达的关系%Relationship between the Expression of Estrogen Receptor α and Basal Cell Dedifferentiation in Benign Prostatic Hyperplasia

    Institute of Scientific and Technical Information of China (English)

    邵瑞; 周颖; 刘文华; 杜小玲; 石建党; 张琚

    2013-01-01

    Enhanced estrogen effect is believed to play an important role in the pathological benign prostate hyperphasia (BPH).The the expression of epithelium cytokeratin (CK),nonmuscle myosin heavy chain (NMMHC),proliferating cell nuclear antigen (PCNA) and estrogen receptor α (ERα) in 20 cases of BPH and 10 cases of normal prostate (NP) tissue samples was studied.Data from immunohistochemical staining showed that the expression of stem/progenitor cells related marker include CK14 and CK5,the dedifferentiation related marker NMMHC,as well as ERα are all increased significantly in the basal cells in BPH tissues.Data also suggest that CK14,NMMHC and ERα have a colocalization relationship,and the colocalization of these three proteins are further proved by immunofluorescence double staining experiment.These results indicate that basal cells dedifferentiated to stem-like cells in BPH tissues and the enhanced estrogen effect induced basal cell dedifferentiation is an essential mechanism for the initiation and development of BPH.%雌激素效应增强在良性前列腺增生(BPH)发生发展中发挥了重要作用.收集20例BPH和10例正常前列腺(NP)组织标本,采用免疫组化染色和免疫荧光方法研究良性前列腺增生组织中上皮细胞角蛋白(CK)、非肌型肌球蛋白(NMMHC)、增殖细胞核抗原(PCNA)和雌激素受体α(ERα)的表达及其定位关系.结果表明,与NP相比,在BPH组织基底细胞中与干/祖细胞相关的CK14和CK5以及与去分化相关的NMMHC和ERα的表达明显增加;而且CK14、NMMHC和ERα有共定位的关系.BPH组织中部分基底细胞去分化为干性细胞;雌激素效应增加使基底细胞发生去分化是BPH发生和发展的重要机制.

  6. Two cases study of benign prostatic hyperplasia combined with suppurative prostatitis%合并化脓性炎的良性前列腺增生2例病例分析

    Institute of Scientific and Technical Information of China (English)

    丁小举; 张卫星; 王朝亮; 李锐; 张天标

    2014-01-01

    Objective To analyze the clinical feature and diagnosis of BPH combined with suppurative prostatitis. Methods The clinical data of two cases of BPH combined with suppurative prostatitis were retrospectively analyzed. Results The age of the two patients was less than 60. They came to hospital because of AUR with fever and infection. The tPSA of them were greater than 100ng/mL,the US prompt BPH with calcification,and the MRI/MRS prompt BPH with prostatitis.The post-operation routine pathology both diagnosed BPH with focal suppurative prostatitis. Conclusion When the tPSA of the BPH patients is significantly higher,accompanied with diagnosed infection and prostatitis,the treatment should be anti-infective therapy and dynamic monitoring serum PSA,so as to decrease the misdiagnosis rate.%目的:探讨良性前列腺增生合并前列腺化脓性炎的临床表现及诊断。方法回顾分析良性前列腺增生合并化脓性炎2例临床资料。结果2例患者均<60岁,均以发热、感染并急性尿潴留就诊。2例患者tPSA均>100ng/mL,彩超提示前列腺增生并钙化。前列腺MRI及前列腺波谱分析(MRI/MRS)提示良性前列腺增生、前列腺炎。术后常规病理结果示良性前列腺增生并局灶化脓性炎。结论当前列腺增生患者PSA明显升高,客观检查提示前列腺炎、感染时,应积极控制感染、动态监测PSA,以降低前列腺癌的误诊率。

  7. 胚胎干细胞基因Nanog,Oct-4基因在前列腺癌中的表达及意义%Expression of Embryonic Stem Cell Genes Nanog and Oct-4 in Human Prostate Cancer and Its Clinical Significance

    Institute of Scientific and Technical Information of China (English)

    熊靓; 孙庭

    2011-01-01

    [目的]探讨Nanog基因和Oct-4基因在良性前列腺增生(BPH),前列腺上皮内瘤变(PIN)及前列腺癌(PCa)中的表达及其在PCa发生发展中的作用.[方法]用免疫组织化学法检测BPH(n=30)、PIN(n=8)及PCa(n=94)中胚胎干细胞相关基因Nanog,Oct-4的表达水平,并比较三者之间的差异.[结果]Nanog基因和Oct-4基因在BPH和PIN组织中无明显差异(P>0.05).PCa组织中的Nanog基因和Oct-4基因明显高于BPH及PIN(P<0.05).随着PCa组织Gleason评分的增加,肿瘤中干细胞相关基因Nanog,Oct-4的表达也明显增多(P<0.05).[结论]Nanog,Oct-4基因在前列腺癌中均有表达,其表达水平与肿瘤的组织学分级相关.%[Objcctivc]To explore the expression of Nanog and Oct-4 genes in benign prostatic hypcrplasia(BPH) , prostatic intracpithclial ncoplasm(PIN) and prostatic canccr(PCa) and the role in the genesis and development of PCa.[Methods]The expression of embryonic stem cell genes Nanog and Oct-4 were detected by immunohistochemistry method in 30 BPH patients, 8 PIN patients and 94 PCa patients.The difference among three groups was compared.[Results! There was no obvious difference in the expression of Nanog and Oct-4 between BPH and PIN tissucs( P >0.05).The expression of Nanog and Oct-4 in Pea tissues were obviously higher than those in BPH and PIN tissucs( P >0.05).With the increasing of Glcason score, the expression of Nanog and Oct-4 incrcascd( P <0.05).[Conclusion! Nanog and Oct-4 genes arc expressed on prostatic cancer, and their expression levels arc related to the histological grade of the tumor.

  8. Comparative effectiveness of oral drug therapies for lower urinary tract symptoms due to benign prostatic hyperplasia: a systematic review and network meta-analysis.

    Directory of Open Access Journals (Sweden)

    Xinghuan Wang

    Full Text Available Lower urinary tract symptoms (LUTS due to benign prostatic hyperplasia (BPH are common in elder men and a number of drugs alone or combined are clinically used for this disorder. But available studies investigating the comparative effects of different drug therapies are limited. This study was aimed to compare the efficacy of different drug therapies for LUTS/BPH with network meta-analysis.An electronic search of PubMed, Cochrane Library and Embase was performed to identify randomized controlled trials (RCTs comparing different drug therapies for LUTS/BPH within 24 weeks. Comparative effects were calculated using Aggregate Data Drug Information System. Consistency models of network meta-analysis were created and cumulative probability was used to rank different therapies.A total 66 RCTs covering seven different therapies with 29384 participants were included. We found that α-blockers (ABs plus phosphodiesterase 5 inhibitors (PDE5-Is ranked highest in the test of IPSS total score, storage subscore and voiding subscore. The combination therapy of ABs plus 5α-reductase inhibitors was the best for increasing maximum urinary flow rate (Qmax with a mean difference (MD of 1.98 (95% CI, 1.12 to 2.86 as compared to placebo. ABs plus muscarinic receptor antagonists (MRAs ranked secondly on the reduction of IPSS storage subscore, although monotherapies including MRAs showed no effect on this aspect. Additionally, PDE5-Is alone showed great effectiveness for LUTS/BPH except Qmax.Based on our novel findings, combination therapy, especially ABs plus PDE5-Is, is recommended for short-term treatment for LUTS/BPH. There was also evidence that PDE5-Is used alone was efficacious except on Qmax. Additionally, it should be cautious when using MRAs. However, further clinical studies are required for longer duration which considers more treatment outcomes such as disease progression, as well as basic research investigating mechanisms involving PDE5-Is and other

  9. Efficacy and safety of combined therapy with terazosin and tolteradine for patients with lower urinary tract symptoms associated with benign prostatic hyperplasia: a prospective study

    Institute of Scientific and Technical Information of China (English)

    YANG Yong; ZHAO Xiao-feng; LI Han-zhong; WANG Wei; ZHANG Yong; XIAO He; ZHANG Xin

    2007-01-01

    Background The primary objectives of the treatment for the lower urinary tract symptoms (LUTS) associated with benign prostatic hyperplasia (BPH) are to produce rapid, sustained, and safe improvements in the symptoms that affect the quality of life in the majority of men over 50. In this study, we evaluated the efficacy and safety of the combined therapy with terazosin (α1-adrenergic receptor antagonist) and tolterodine (anticholinergic agent) for LUTS associated with BPH.Methods This combination study included 69 patients diagnosed with LUTS associated with BPH based on the International Prostate Symptom Scores (IPSS), urinary flow rate, prostate volume, urinary residual, and their serum prostate-specific antigen levels. Initially, 191 patients were treated with terazosin 2 mg once daily for one week. Those patients with continued LUTS after the initial treatment were allocated randomly into two groups: terazosin group (n=36)in which patients were treated with terazosin 2 mg once daily for six weeks, and combination group (n=33) in which patients were treated with both terazosin 2 mg once daily and tolterodine 2 mg twice daily for 6 weeks.Results The IPSS were significantly improved in both groups after treatment, and the reduction of IPSS in the combination group was significantly greater than that in the terazosin group (P<0.01). A decrease in urgency, frequency and nocturia were the main contributory factors causing the reduction of IPSS in the combination group. The differences about the peak urinary flow rate and the residual urine from the baseline values were noted in both groups after treatment,but were not significant between the two groups. The incidence of adverse effects in the combination group was higher than that in the terazosin group. As expected the most common adverse effect was mouth dryness which was associated with anticholinergic drugs such as tolterodine.Conclusions Patients with LUTS associated BPH appear the improved IPSS after

  10. AcEST: BP919214 [AcEST

    Lifescience Database Archive (English)

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  11. Prostate size correlates with fasting blood glucose in non-diabetic benign prostatic hyperplasia patients with normal testosterone levels.

    Science.gov (United States)

    Kim, Won Tae; Yun, Seok Joong; Choi, Young Deuk; Kim, Gi-Young; Moon, Sung-Kwon; Choi, Yung Hyun; Kim, Isaac Yi; Kim, Wun-Jae

    2011-09-01

    We evaluated the correlations between BMI, fasting glucose, insulin, testosterone level, insulin resistance, and prostate size in non-diabetic benign prostatic hyperplasia (BPH) patients with normal testosterone levels. Data from 212 non-diabetic BPH patients with normal testosterone levels, who underwent transurethral resection of the prostate (TURP) due to medical treatment failure, were evaluated retrospectively. Patients with prostate specific antigen (PSA) levels of ≥ 3 ng/mL underwent multicore transrectal prostate biopsy before TURP to rule out prostate cancer. Patients with diabetes mellitus (DM) or serum testosterone levels of Prostate size correlated positively with age (r = 0.227, P 0.05). Testosterone level inversely correlated with BMI (r = -0.327, P prostate size, PSA, or fasting glucose level (each P > 0.05). Upon multiple adjusted linear regression analysis, prostate size correlated with elevated PSA (P prostate hyperplasia.

  12. 前列腺癌骨转移灶中BMP-2、BMP-4、BMP-7的表达及临床意义%Expression of BMP-2, BMP-4 and BMP-7 in human metastatic prostate cancer and their clinical significance

    Institute of Scientific and Technical Information of China (English)

    宫丽华; 孙晓淇; 刘宝岳; 黄啸原

    2012-01-01

    目的 检测BMP-2、BMP-4、BMP-7在前列腺癌骨转移灶中的表达,探讨其在前列腺癌成骨性转移中的作用.方法 采用免疫组化EnVision法检测28例前列腺癌骨转移病例及17例良性前列腺增生(benign prostate hyperplasia,BPH)病例中BMP-2、BMP-4、BMP-7的表达并对其进行对比分析.结果 BMP-2在所有前列腺癌骨转移灶及BPH病例中均表达,二者中其阳性率及表达强度无明显差异(P>0.05).BMP-4在前列腺癌骨转移灶及BPH中的阳性率无明显差异(P>0.05),但在前者中BMP-4的表达强度明显高于后者(P<0.05).BMP-7在前列腺癌骨转移灶中的阳性率及表达强度均明显高于BPH (P<0.05).在BPH的阳性表达病例中,BMP-2、BMP-4、BMP-7细胞质与细胞核同时阳性的表达率分别为13.3%、7.1%和11.1%,在前列腺癌骨转移灶的阳性表达病例中,BMP-2、BMP-4、BMP-7细胞质与细胞核的同时阳性的表达率均为100%,且细胞核的表达强度明显高于细胞质.结论 BMP-4、BMP-7在前列腺癌骨转移灶中高表达,提示其在前列腺癌的成骨性转移中可能起重要作用.%Purpose To analyze the expression of BMP-2, BMP-4, BMP-7 in prostate cancer with bone metastasis. Methods Iininu-nohistochemical technique was used to detect the expression of BMP-2, BMP-4 and BMP-7 in the bone osteobastic metastastic lesions of prostate cancer and the benign prostate hyperplasia ( BPH ). Results The expression of BMP-2 was detected in all samples and there was no difference between prostate cancer and BPH. The intensity of positivity of BMP-4 was higher in prostate cancer than that in BPH ( P < 0. 05 ), but the difference of positive rate between these two groups showed no significance. The intensity of positivity and the positive rate of BMP-7 were higher in prostate cancer than that in BPH ( P < 0. 05 ). The positive rate of staining involving both cyto-plasm and nuclear in BMP-2, BMP-4 and BMP-7-positive samples in BPH was respectively 13. 3

  13. 前列腺增生合并组织学炎症患者临床及尿动力学特征分析%Clinical and Urodynamic Characteristics of Patients with Benign Prostatic Hyperplasia Combined with Histological Prostatitis

    Institute of Scientific and Technical Information of China (English)

    庞然; 高筱松; 卢建新; 李理; 杨志强

    2014-01-01

    目的:探讨前列腺增生(BPH)合并组织学前列腺炎(HP)患者的临床及尿动力学特征。方法:将189例BPH接受经尿道逆行前列腺电切术治疗的患者,依据病理诊断分为单纯BPH组和BPH合并HP组,在匹配年龄、体重指数和前列腺体积后,每组38例;比较两组国际前列腺症状评分(IPSS)、最大尿流率、最大膀胱容量、残余尿量、最大逼尿肌压力和最大尿道压力等尿动力学指标,以及膀胱逼尿肌过度活动发生率间的差异。结果:与单纯BPH组相比,BPH合并HP组具有更高的IPSS总分和IPSS储尿期评分(P<0.05),较小的最大膀胱容量(P<0.001)和更高的膀胱逼尿肌过度活动的发生率(P<0.05)。结论:合并HP会加重BPH患者的储尿期症状,导致尿动力学中最大膀胱容量的降低和逼尿肌过度活动的发生。%Objective To investigate the clinical and urodynamic characteristics in patients with benign prostatic hyperplasia (BPH) combined with histological prostatitis (HP). Methods One hundred and 89 pa-tients underwent transurethral retrograde prostatectomy (TURP) due to BPH in Guang'an Men Hospital were ret-rospectively reviewed and divided into two groups, BPH group and BPH with HP group, based on pathological characteristics of tissue samples. After matching age, body mass index and prostate volume, each group had 38 cases. The International prostate symptom score (IP-SS) and urodynamic features including maximum urine flow, maximum cystometric capacity, maximum urethral pressure, maximum detrusor pressure and post-void residual urine volume were compared between the two groups. Moreover, the proportions of patients with involuntary detrusor contractions were analyzed in both groups. Results Patients in BPH with HP group presented significant higher IPSS total scores, IPSS storage sub-scores and lower maximum cystometric capacity than the counterparts in BPH group. Patients in BPH with

  14. 合并前列腺炎症的良性前列腺增生组织中Integrinα2β1/CD133表达及其意义%Expressions of Integrinα2β1 and CD133 in benign prostatic hyperplasia complicated by prostatitis and their significance

    Institute of Scientific and Technical Information of China (English)

    丁奇; 苗文隆; 刘硕; 畅继武; 杨宇明

    2011-01-01

    Objective: To study the expressions of Integrinα2β1 and CD133 in benign prostatic hyperplasia (BPH) complicated by prostatitis and their significance. Methods: Specimens were obtained from 56 BPH patients undergoing transvesical prostatectomy. Paraffin sections of the specimens were subjected to HE staining for pathological examination of inflammatory changes under the light microscope. Twenty-four patients with simple BPH were included in Group A, and the other 32 with BPH complicated with prostatitis in Croup B. The expressions of Integrincα2β1 and CD133 in the prostatic tissues of the two groups were determined by immunohisto-chemistry, Western blotting and IPP6.0 image analysis software. Results: The expressions of Integrinα2β1, and CD133 were significantly higher in Group B than in A ( P < 0.05 ) , and so were the mean relative value of the optical density of Integrinα2β1, (0.29 ± 0.18 vs 0.04 ± 0.03) and that of CD133 (0.08 ± 0.07 vs 0.002 0 ± 0.001 8) (P < 0.05). Conclusion: Inflammation can up-regulate the expressions of Integrinα2β1 and CD133 in BPH tissue.%目的:探讨合并前列腺炎症的良性前列腺增生(BPH)组织中Integrinα2β1/CD133表达及其意义.方法:收集56例行经膀胱前列腺摘除手术的BPH患者的前列腺标本,常规HE染色后观察其合并炎症情况,其中单纯BPH(24例)为A组,合并前列腺炎症的BPH(32例)为B组.采用免疫组化SP法及Western印迹法检测Integrinα2β1/CD133在两组前列腺组织中表达差异.Image-Pro@ Plus 6.0图像分析软件对结果进行分析.结果:免疫组化Mattern积分结果显示:两组中Integrinα2β1/CD133表达有显著差异(P<0.05).Western免疫印迹显示:B组Integrinαt2β1平均相对光密度值高于A组[(0.29±0.18)vs (0.04±0.03)],P<0.05;B组CD133平均相对光密度值高于A组[(0.08±0.07) vs(0.002 0±0.001 8)],P<0.05.提示B组中Integrinα2β1/CD133表达较A组显著升高.结论:炎症可以促进BPH组织中Integrinα2β1

  15. The correlation between lipid abnormality and benign prostatic hyperplasia in men receiving physical examination%良性前列腺增生与血脂异常

    Institute of Scientific and Technical Information of China (English)

    林秀芳; 曾莉; 黄昶荃; 董碧蓉

    2009-01-01

    目的 探讨体检人群中老年人良性前列腺增生(BPH)与血脂异常的相关性. 方法 选择在我院体检的中老年男性共401例,通过现场询问病史,国际前列腺症状评分(IPSS),直肠指诊,经直肠B超分为两组:(1)BPH组192例;(2)非BPH组209例.比较两组血脂水平及危险分层差异. 结果 两组血脂水平[三酰甘油(TG):t=0.388,P=0.698;总胆固醇(TC):t=0.449,P=0.654;低密度脂蛋白胆固醇(LDL-C):t=0.151,P=0.880;高密度脂蛋白胆固醇(HDL-C):t=0.628,P=0.531)]、心血管病综合危险的评价分层(X2=4.094,P=0.251)差异均无统计学意义;IPSS评分与血脂异常亦无明显相关性(TG:X2=5.855,P=0.054;TC:X2=3.813,P=0.149;LDL-C:X2=1.704,P=0.427;HDL-C:X2=3.289,P=0.193). 结论 BPH并存血脂异常临床多见,但在BPH下尿路症状以轻中度为主的体检人群中.二者之间的相关性不如以需手术治疗的中重度BPH患者为纳入人群的类似研究关系明确,其机理尚有待进一步研究证明.%Objective To investigate the relationship between lipid abnormality and benign prostatic hyperplasia (BPH) in men receiving physical examination. Methods Four hundred and one people who participated health examination in our hospital in May 2008 were included in the study. They were divided into two groups according to history of disease, IPSS score, the results of digital rectal examination and transrectal ultrasound: BPH group (192 cases) and no BPH group (209 cases). The blood lipid level and risk factors were compared in two groups. Results There were no statistical differences in blood lipid level and risk stratification of cardiovascular disease between the two groups (TG:P=0. 698;TC:P=0. 654;LDL-C:P=0. 880; HDL-C:P=0. 531; risk stratification: P= 0. 251). IPSS score had no obvious correlation with blood lipid level (TG: P = 0. 054 ; TC: P =0. 149; LDL-C: P = 0. 427; HDL-C:P=0. 193) . Conclusions BPH complicated with lipid abnormality is common in the clinics, but the

  16. THE ROLES OF DETOXIFYING ENZYMES AND AChE INSENSITIVITY IN METHAMIDOPHOS RESISTANCE DEVELOPMENT AND DECLINE IN NILAPARVATA LUGENS

    Institute of Scientific and Technical Information of China (English)

    Ze-wenLiu; Zhao-junHan; Ling-chunZhang

    2003-01-01

    Methamidophos resistance of brown planthopper (Nilaparvata lugens Stal, BPH) was selected in laboratory. After successive selection for 9 generations, the selection was ceased by rearing BPH without contact with any insecticide for 9 generations. In the full course, the successive changes of esterase activity, MFO activity, GSTs activity and AChE insensitivity were analyzed. The results showed that the change of esterase activity was high correlated with that of methamidophos in the full course, which indicated that esterase played very important role both in the resistance development and in the resistance decline. However, the change of AChE insensitivity only significantly correlated with that of resistance in the development stage, and the change of MFO activity or GSTs activity only significantly correlated with that of the resistance in the decline stage, which indicated the changes of AChE insensitivity, MFO activity or GSTs activity only played some roles in different stages of the resistance change.

  17. Zn-Al LAYERED DOUBLE HYDROXIDE PILLARED BY DIFFERENT DICARBOXYLATE ANIONS

    Directory of Open Access Journals (Sweden)

    S. Gago

    2004-12-01

    Full Text Available Zn-Al layered double hydroxides (LDHs intercalated by terephthalate (TPH and biphenyl-4,4'-dicarboxylate (BPH anions have been synthesized by direct co-precipitation from aqueous solution. The Zn/Al ratio in the final materials was 1.8. The products were characterized by powder X-ray diffraction, thermogravimetric analysis, FTIR and FT Raman spectroscopy, and MAS NMR spectroscopy. The basal spacing for the TPH-LDH intercalate was 14.62 Å, indicating that the guest anions stack to form a monolayer with the aromatic rings perpendicular to the host layers. For the LDH intercalate containing BPH anions, a basal spacing of at least 19.2 Å would be expected if the anions adopted an arrangement similar to that for the TPH anions. The observed spacing was 18.24 Å, suggesting that the anions are tilted slightly with respect to the host layers.

  18. Human benign prostatic hyperplasia in organ culture: studies on iododeoxyuridine uptake

    Energy Technology Data Exchange (ETDEWEB)

    Masters, J.R.; O' Donoghue, E.P.

    1983-01-01

    Prostate tissue architecture and function may be retained in vitro using organ culture. However, the application of the technique is limited due to the lack of simple means for measuring tissue response, for instance to factors controlling cell growth. It is confirmed in this study that measuring the uptake of the radioactive precursor of DNA, 5-(/sup 125/I)-iodo-2'-deoxyuridine, is simple, rapid, and reproducible provided the heterogeneity of benign prostatic hyperplasia (BPH) is taken into account. Factors affecting the reproducibility (numbers of replicate explants and dishes), specificity (removal of unbound radioactivity and uptake into killed tissue), and sensitivity (radioactive concentration and exposure period) of this technique were investigated. A particular advantage of this approach is that histological assessments are made on the same tissue used for biochemical measurements. Furthermore, the method is sufficiently simple to permit serial investigations chronologically concerning factors, such as hormones, controlling BPH cell growth.

  19. High-Intensity Focused Ultrasound (HIFU) Using Sonablate® Devices for the Treatment of Benign Prostatic Hyperplasia and Localized Prostate Cancer: 18-year experience

    Science.gov (United States)

    Uchida, Toyoaki

    2011-09-01

    From 1993 to 2010, we have treated 156 patients benign prostatic hyperplasia (BPH) and 1,052 patients localized prostate cancer high-intensity focused ultrasound (HIFU). Four different HIFU devices, SonablateR-200, SonablateR-500, SonablateR-500 version 4 and Sonablate® TCM, have been used for this study. Clinical outcome of HIFU for BPH did not show any superior effects to transurethral resection of the prostate, laser surgery or transurethral vapolization of the prostate. However, HIFU appears to be a safe and minimally invasive therapy for patients with localized prostate cancer, especially low- and intermediate-risk patients. The rate of clinical outcome has significantly improved over the years due to technical improvements in the device.

  20. Detection of benign prostatic hyperplasia nodules in T2W MR images using fuzzy decision forest

    Science.gov (United States)

    Lay, Nathan; Freeman, Sabrina; Turkbey, Baris; Summers, Ronald M.

    2016-03-01

    Prostate cancer is the second leading cause of cancer-related death in men MRI has proven useful for detecting prostate cancer, and CAD may further improve detection. One source of false positives in prostate computer-aided diagnosis (CAD) is the presence of benign prostatic hyperplasia (BPH) nodules. These nodules have a distinct appearance with a pseudo-capsule on T2 weighted MR images but can also resemble cancerous lesions in other sequences such as the ADC or high B-value images. Describing their appearance with hand-crafted heuristics (features) that also exclude the appearance of cancerous lesions is challenging. This work develops a method based on fuzzy decision forests to automatically learn discriminative features for the purpose of BPH nodule detection in T2 weighted images for the purpose of improving prostate CAD systems.

  1. In situ localization of proteinase inhibitor mRNA in rice plant challenged by brown planthopper

    Institute of Scientific and Technical Information of China (English)

    2003-01-01

    Proteinase inhibitor (PI) mRNA was localized by in situ hybridization in tissue sections of root, stem and leaf of the resistant rice (B5) plant fed by brown planthopper nymphs. In the rice material without BPH feeding, PI gene was expressed in the root, stem and leaf, while the abundance of PI mRNA was low. In the rice material fed by BPH, PI gene was expressed substantially in the parenchyma of rice stem and leaf, but weakly in the root. The results indicated that the PI gene was up-regulated in the rice plant challenged by brown planthopper. For the first time, we reported the expression changes of proteinase inhibitor gene in plant which was infested by a piercing/sucking insect.

  2. Estimation of Genetic Effect for 2N Pollen Producing Diploid Hybrids

    Institute of Scientific and Technical Information of China (English)

    1999-01-01

    Three 2x clones with 2n pollen production, BPH32-2,BPH32-5 and W5295,7, were each mated with three 4x cultivars, Raritan, Superior and Trent, in an incomplete diallel cross design. Estimates of various heritability for tuber yield per plant are that h2m is 37.20%,h2f 16.30%,h2mf 23.30%,h2B 76.70% and h2N 53.40%. Estimates of general combining ability(GCA) and specific combining ability(SCA) are 69.60% and 30.30%,respectively. The results suggest that additive effect of genes for tuber yield is obvious. The 2n pollen producing diploid hybrids have good breeding values for yield ,earliness and starch content.

  3. Economic issues and the management of benign prostatic hyperplasia.

    Science.gov (United States)

    Holtgrewe, H L

    1995-09-01

    Enormous financial resources are expended worldwide on the treatment of the urologic complications and symptoms induced by benign prostatic hyperplasia (BPH). Even for its surgical management, where the best data exist, current international accounting of these expenditures remains very poorly documented. On February 8, 1994, the Department of Health and Human Services of the US government released clinical guidelines for the diagnosis and management of BPH. Imaging of the upper urinary tract as a routine diagnostic procedure is not recommended in these guidelines unless a comorbidity indicating its need exists. Diagnostic cystoscopy to assist in the decision of the need to treat is not recommended. Adherence to these two principles along with adherence to the strategies of management presented in the guidelines and discussed herein has the potential of achieving profound financial savings without impairing quality of care worldwide.

  4. Linear trinuclear cobalt(II) single molecule magnet.

    Science.gov (United States)

    Zhang, Yuan-Zhu; Brown, Andrew J; Meng, Yin-Shan; Sun, Hao-Ling; Gao, Song

    2015-02-14

    The introduction of NaBPh(4) into a methanolic solution of CoCl(2)·(6)H(2)O and 2-[(pyridine-2-ylimine)-methyl]phenol (Hpymp) afforded {[Co(II)(3)(pymp)(4)(MeOH)(2)][BPh(4)](2)}·(2)MeOH (1) with a centro-symmetrically linear trinuclear structure. Magnetic analysis of 1 exhibited significant intracluster ferromagnetic exchange (2.4 cm(-1)) and slow relaxation of magnetization in both zero and non-zero static fields below 5 K, giving the first [Co(II)(3)] single molecule magnet with an effective energy barrier of 17.2(3) cm(-1) under a 500 Oe dc field.

  5. [pH sensors based on rubbery ormosils preparation and their spectrum studies].

    Science.gov (United States)

    Chen, Xi; Dai, Yuan-jing; Li, Wei; Zhuang, Zhi-xia; Wang, Xiao-ru

    2002-02-01

    A new type of methyl substituted ormosils as a matrix for bromophenol blue (BPhB) and bromocresol green (BCG) is described. The new ormosils combine features of classical TEOS sol-gel material such as solvability in organic solvent and those of sol-gel glasses such as transparent and a porous structure, the ormosils also make a good mechanical stability. The influence of the conditions during the polymerisation process on the photochemical properties of BPhB and BCG has been studied. This sol-gel material was wed to immobilize pH-sensitive absorption dyes, bromothymol blue and bromocresol green, to prepare pH sensing films. The several aspects of the sensing films, including the leaching of the dye from gel, response time to different pH buffer solution, absorption spectra and the improvement of the immobilization of the dyes to filmo, were also discussed.

  6. The value of appropriate assessment prior to specialist referral in men with prostatic symptoms.

    LENUS (Irish Health Repository)

    Quinlan, M R

    2012-02-01

    BACKGROUND: Referrals to Urology OPD of men with a likely diagnosis of BPH are common. AIMS: To review referrals to OPD of men with lower urinary tract symptoms (LUTS) to establish how many could have been managed without specialist assessment. METHODS: We reviewed records of 200 male patients referred to OPD with LUTS. We assessed whether the referral source had performed digital rectal examination (DRE), International Prostate Symptom Score (IPSS), Bother Score or PSA level. RESULTS: 74% of patients were referred by GPs. In 31.5% of cases DRE was performed prior to referral. One GP had completed an IPSS, none a Bother Score. 96% had a PSA checked before OPD. Ultimately, 88.5% of our patients were diagnosed with BPH. CONCLUSIONS: With better pre-assessment in the form of DRE, IPSS and Bother Score, allied to a PSA check, many patients with LUTS could be managed in a primary care setting.

  7. Chromosomal deletion, promoter hypermethylation and downregulation of FYN in prostate cancer

    DEFF Research Database (Denmark)

    Sørensen, Karina Dalsgaard; Borre, Michael; Ørntoft, Torben Falck

    2008-01-01

    Loss of heterozygosity (LOH) at 6q is a frequent chromosomal aberration in prostate adenocarcinoma; however, a possible target gene remains to be identified. Findings in this study indicate that the FYN tyrosine kinase gene at 6q21 is a new candidate tumor suppressor in prostate cancer. Initially......, single nucleotide polymorphism microarray analysis of 40 microdissected prostate adenocarcinoma samples revealed 25% LOH at the FYN locus. Furthermore, Western blot analysis and real-time reverse transcriptase PCR (RT-PCR) showed significantly lower FYN expression in prostate cancer tissue than in benign...... prostate hyperplasia (BPH), as well as in 6 prostate adenocarcinoma cell lines compared with that in BPH-1 cells. By immunohistochemistry, FYN protein was detected in nonmalignant prostate epithelium, but not in cancerous glands. Moreover, genomic bisulfite sequencing revealed frequent aberrant methylation...

  8. Die Transurethrale Nadel-Ablation der Prostata (TUNA

    Directory of Open Access Journals (Sweden)

    Stark L

    1998-01-01

    Full Text Available 25 Patienten im Alter von 56?92 Jahren (Durchschnitt: 74,9 mit symptomatischer BPH wurden mittels TUNA therapiert. Das Prostatavolumen betrug 19-160 ml (durchschnittlich 56,6. 14 Patienten (56 % wurden aufgrund eines akuten Harnverhaltes therapiert, 9 Patienten (36 % hatten Restharn zwischen 30 und 700 ml, 2 Patienten (8 % wurden wegen irritativer Symptome behandelt. Bei 4 Patienten wurde die TUNA 2-3 mal wiederholt, bei 5 Patienten kombinierten wir die TUNA mit einer transurethralen Resektion einer Mittellappenhyperplasie. Von den 25 Patienten waren 17 Hochrisikopatienten (ASA III-IV. Die Erfolgsquote betrug 68 %. 6 Patienten (24 % benötigten weiterhin eine transurethrale oder suprapubische Harnableitung, 2 Patienten benötigten eine weitere invasive Therapie. Unsere Ergebnisse bestätigen die TUNA als wirkungsvolle und äußerst nebenwirkungsarme Therapiemöglichkeit bei Patienten mit symptomatischer BPH.

  9. Atypical post-finasteride syndrome: A pharmacological riddle

    Directory of Open Access Journals (Sweden)

    Anita K Gupta

    2016-01-01

    Full Text Available Finasteride and dutasteride are commonly used 5-alpha reductase inhibitors. While finasteride is a selective inhibitor of 5-alpha reductase Type II, dutasteride inhibits 5- alpha reductase Type I and II. The United States Food and Drug Administration approved the use of finasteride for benign prostatic hypertrophy (BPH as well as androgenic alopecia (AGA while dutasteride is approved only for BPH. Off-label use of dutasteride is not uncommon in AGA as well. Although the postfinasteride syndrome (PFS is a well-established entity, its symptomatology is quite variable. Here, we describe a case of an atypical PFS in a patient treated with dutasteride and finasteride for AGA. The multisystem involvement and irreversible nature of this case warrant its reporting.

  10. α-受体阻断药在治疗良性前列腺增生中的地位和作用

    Institute of Scientific and Technical Information of China (English)

    张丽霞

    2002-01-01

    @@ 随着社会呈现老龄化趋势,老年男性良性前列腺增生症(benign prostate hypertrophy,BPH)的发病率逐年增加[1].据统计,61~70岁老年男性的发病率约为70%[2],至80岁时,病理性BPH的发病率可达90%[3].因而,许多国际和国内学者均将BPH列为主要研究课题之一.在WHO积极支持下,1991年成立了"良性前列腺增生国际协调委员会".如何正确诊断、治疗BPH,如何提高BPH患者的生活质量,已成为亟待解决的问题.

  11. Ttransurethral electro- raporization excision for treatment of 30 cases of benign prostatic hypertrophy%经尿道前列腺气化电切治疗前列腺增生症30例

    Institute of Scientific and Technical Information of China (English)

    陈建中; 魏明; 秦有道

    2001-01-01

    目的探讨经尿道气化电切治疗前列腺增生症(BPH)的疗效.方法采用经尿道前列腺气化电切治疗BPH 30例.结果患者症状明显改善,术后3个月随访,国际前列腺症状评分平均8.5分,残余尿量平均25 ml,未出现严重并发症.结论经尿道前列腺气化电切术是一种安全性高,并发症少,疗效确切的新的手术方法.

  12. Tamsulosin-induced severe hypotension during general anesthesia: a case report

    Directory of Open Access Journals (Sweden)

    Khan Fauzia

    2010-11-01

    Full Text Available Abstract Introduction Tamsulosin, a selective α1-adrenergic receptor (α1-AR antagonist, is a widely prescribed first-line agent for benign prostatic hypertrophy (BPH. Its interaction with anesthetic agents has not been described. Case presentation We report the case of 54-year-old Asian man undergoing elective left thyroid lobectomy. The only medication the patient was taking was tamsulosin 0.4 mg for the past year for BPH. He developed persistent hypotension during the maintenance phase of anesthesia while receiving oxygen, nitrous oxide and 1% isoflurane. The hypotension could have been attributable to a possible interaction between inhalational anesthetic and tamsulosin. Conclusion Vigilance for unexpected hypotension is important in surgical patients who are treated with selective α1-AR blockers. If hypotension occurs, vasopressors that act directly on adrenergic receptors could be more effective.

  13. Portable {sup 90}SR/{sup 90}Y prostatic hyperplasia applicators

    Energy Technology Data Exchange (ETDEWEB)

    Cai, Shanyu; Tang, Kejian; Zhou, Changling [China Institute of Atomic Energy (China); Li, Zhi [Zhelimumen Hospital (China)

    1998-07-01

    In order to seek a new method of curing the benign prostatic hyperplasia (BPH), two different kinds of {sup 9} {sup 0}Sr/9{sup 0}Y intracavity applicators, including a 'urethra-type' and a 'rectum-type', have been developed in China since 1991. The structural design and radiation characteristics of the {sup 90}Sr/{sup 90}Y prostatic hyperplasia applicator are given in this paper. The hypertrophic prostate gland can be irradiated through the wall of the urethra or rectum by {sup 90}Sr/{sup 90}Y beta rays and small quantity of bremsstrahlung radiation from the applicator. Clinical tests indicate that the {sup 90}Sr/{sup 90}Y prostatic hyperplasia applicators provide a safe, effective, non-invasive and economical therapeutic method for BPH. It is especially applicable for old and high-risk patients. (author)

  14. Androgen-independent effects of Serenoa repens extract (Prostasan®) on prostatic epithelial cell proliferation and inflammation

    DEFF Research Database (Denmark)

    Iglesias-Gato, Diego; Carsten, Tober; Vesterlund, Mattias;

    2012-01-01

    Extracts from Serenoa repens are widely used for the treatment of benign prostatic hyperplasia (BPH) and traditionally for prostatitis. In the present study we evaluated the biological effects of Serenoa repens extract (Prostasan®) on prostate cells beyond its known antiandrogenic actions....... Prostasan® inhibited epidermal growth factor (EGF) and lipopolysaccharide (LPS) induced proliferation of the prostatic epithelial, androgen independent cell line PC-3. At effective concentrations of 50 µg/mL, Prostasan® partly displaced EGF from EGF receptor (EGFR) but fully blocked EGF-induced cell......-induced secretion of IL-12 and inhibited MCP-1 and granulocyte-macrophage colony-stimulating factor (GM-CSF) production in the presence of LPS on PC-3 cells. Taken together, our results suggest that S. repens extracts, in addition to other reported effects on BPH development and prostatitis, inhibits EGF...

  15. 老年良性前列腺增生 635例分类处理与提高患者生存质量的相关性分析%Relative analysis of classified treatments and improvement of quality of life in 635 cases of benign prostate hyperplasia

    Institute of Scientific and Technical Information of China (English)

    余斌; 张更

    2002-01-01

    Objective To investigate the method and significance of the classified treatment on benign prostate hyperplasia(BPH) patients according to the symptoms and clinical status. Method The clinical data of 635 patients were analyzed retrospectively. Patients were divided into five groups according to their symptoms and each group was treated differently. Results The rate of cure and utility of the class I patients was 75.2% and 96% , respectively.The cure rate of both class II and class III patients was 100% .The utility rate of the class IV patients was 100% .The whole cure rate and utility rate of the 635 patients were 88.3% and 99.2% ,respectively.Conclusion We can classify the BPH patients according to their symptom and clinical status and treat them differently in terms of classification. This measure can help doctors select the optimal treatment time and method and relieve patients' pain to the maximum extent, thus improving patients' quality of life.

  16. Influence of bladder outlet obstruction and detrusor contractility on residual urine in patients with benign prostatic hyperplasia

    Institute of Scientific and Technical Information of China (English)

    张鹏; 武治津; 高居忠

    2003-01-01

    Objective To study the relationship between the degree of bladder outlet obstruction (BOO), detrusor contractility and residual urine in patients suffering from benign prostatic hyperplasia (BPH).Methods In 181 patients with BPH, degree of BOO, detrusor contractility, residual urine caculated from cathetering combined with the difference between the filling and the voiding were recorded and analysized statistically using urodynamic technique.Results Residual urine increased when the detusor contractility was weakened (F=12.134, P=0.001). In patients wih severe BOO, there was no significant difference in residual urine (F=2.386, P=0.071).Conclusions Increased residual urine is mainly resulted from decreased detrusor contractility. BOO has no significant influence on residual urine. Some patients with normal or weakened detrusor contractility may have more residual urine

  17. THE CLINICAL SIGNIFICANCE OF PERCENT FREE PSA IN DIFFERENTIATING PROSTATE CANCER AND BENIGN PROSTATE HYPERPLASIA

    Institute of Scientific and Technical Information of China (English)

    王子明; 张鹏; 种铁; 赵丽华

    2004-01-01

    Objective To evaluate using prostate specific antigen (PSA) and percent free PSA(fPSA) for the diagnosis of prostate cancer(Pca) and benign prostate hyperplasia(BPH). Methods 315 men with BPH and 55 men with Pca were randomly chosen, serum fPSA and total PSA were determined by ELISA and then we compared the sensitivity and specificity of PSA and percent fPSA for the diagnosis of Pca. Results While using PSA and percent fPSA for the diagnosis of prostate cancer, the sensitivity was similar (89.8% vs. 94.5%, P>0.05), but the specificity was significanty different (52.7% vs. 89.8%, P<0.005). Conclusions Using percent fPSA might decrease false-positive and avoid 37.1% negative biopsies as compared with PSA, it is very valuable for the diagnosis of Pca.

  18. Microwave Treatment of Prostate Cancer and Hyperplasia

    Science.gov (United States)

    Arndt, G. Dickey; Ngo, Phong; Carl, J. R.; Raffoul, George

    2005-01-01

    Microwave ablation in the form of microwave energy applied to a heart muscle by a coaxial catheter inserted in a vein in the groin area can be used to heat and kill diseased heart cells. A microwave catheter has been developed to provide deep myocardial ablation to treat ventricular tachycardia by restoring appropriate electrical activity within the heart and eliminating irregular heartbeats. The resulting microwave catheter design, which is now being developed for commercial use in treating ventricular tachycardia, can be modified to treat prostate cancer and benign prostatic hyperplasia (BPH). Inasmuch as the occurrence of BPH is increasing currently 350,000 operations per year are performed in the United States alone to treat this condition this microwave catheter has significant commercial potential.

  19. Relationship Between the Development of Methamidophos Resistance and the Activities of Three Detoxifying Enzymes in Brown Planthopper,Nilaparvata lugens

    Institute of Scientific and Technical Information of China (English)

    LIuZe-wen; HANZhao-jun; ZHANGLing-chun

    2004-01-01

    Methamidophos resistance of brown planthopper(BPH), Nilaparvata lugens was selected in laboratory for 19 generations (F1 to F19). The resistance development in BPH was approximatively shaped as the letter "S" : resistance change was small before the fifth generation and after the fifteenth generation, and the changing pattern was sharp between the fifth and the fifteenth generation. Esterase might play an important role in the resistance development, because the esterase activity and the number of individuals with high activities increased along with the resistance development. The esterase activities of insecticide-sensitive population S, field population F0, its selective generations F, F10 and F15 were highly correlated with the resistance ratios of these generations, and the coefficient was 0.9899. Mixed-function oxidases and giutathione S-transferase also might play some roles in the resistance development, but the big change in the activities of the two detoxifying enzymes both took place before the tenth generation.

  20. Relationship Between the Development of Methamidophos Resistance and the Activities of Three Detoxifying Enzymes in Brown Planthopper, Nilaparvata lugens

    Institute of Scientific and Technical Information of China (English)

    LIU Ze-wen; HAN Zhao-jun; ZHANG Ling-chun

    2004-01-01

    Methamidophos resistance of brown planthopper(BPH), Nilaparvata lugens was selected in laboratory for 19 generations (F1 to F19). The resistance development in BPH was approximatively shaped as the letter "S" : resistance change was small before the fifth generation and after the fifteenth generation, and the changing pattern was sharp between the fifth and the fifteenth generation. Esterase might play an important role in the resistance development, because the esterase activity and the number of individuals with high activities increased along with the resistance development. The esterase activities of insecticide-sensitive population S, field population F0, its selective generations Fs, F10 and F15 were highly correlated with the resistance ratios of these generations, and the coefficient was 0.9899. Mixed-function oxidases and glutathione S-transferase also might play some roles in the resistance development, but the big change in the activities of the two detoxifying enzymes both took place before the tenth generation.

  1. Sexual outcome of patients undergoing thulium laser enucleation of the prostate for benign prostatic hyperplasia.

    Science.gov (United States)

    Carmignani, Luca; Bozzini, Giorgio; Macchi, Alberto; Maruccia, Serena; Picozzi, Stefano; Casellato, Stefano

    2015-01-01

    Treatment of patients with lower urinary tract symptoms (LUTS) secondary to benign prostatic hyperplasia (BPH) may affect the quality of sexual function and ejaculation. The effect of new surgical procedures, which are currently available to treat BPH, on erection and ejaculation, has been poorly studied. This study aimed to assess the effect of thulium laser enucleation of the prostate (ThuLEP) on sexual function and retrograde ejaculation in patients with LUTS secondary to BPH. We performed a prospective study in 110 consecutive patients who had undergone ThuLEP to analyze changes in sexual function and urinary symptoms. To evaluate changes in erection and ejaculation, and the effect of urinary symptoms on the quality of life (QoL), five validated questionnaires were used: the ICIQ-MLUTSsex, MSHQ-EjD, International Index of Erectile Function 5, International Prognostic Scoring System (IPSS) questionnaire, and QoL index of the intraclass correlation coefficients. Patients also underwent IPSS and flowmetry to assess the outcome of flow. Patients were evaluated before surgery and 3-6 months after ThuLEP, whereas those with previous abdominal surgery were excluded. The patients' mean age was 67.83 years. Postoperative urinary symptoms improved after surgery. No significant differences in erectile function before and after surgery were observed. As compared with other techniques described in the literature, the percentage of patients with conserved ejaculation increased by 52.7% after ThuLEP. ThuLEP positively affects urinary symptoms and their effect on the QoL of patients as assessed by questionnaire scores. While endoscopic management of BPH (e.g. transurethral resection of the prostate) causes retrograde ejaculation in most patients, those who undergo ThuLEP have conserved ejaculation and erectile function.

  2. A case of giant prostatic hyperplasia

    OpenAIRE

    Luke Wang; Paul Davis; Kevin McMillan

    2016-01-01

    Benign prostatic hyperplasia (BPH) is one of the most common conditions experienced by aging males and a frequent cause of bladder outlet obstruction and macroscopic haematuria. Giant prostatic hyperplasia (GPH) is an extremely rare form of prostatic hyperplasia. We present a case of a patient with GPH of 800 mL. To our knowledge, this is the fourth largest prostatic hyperplasia ever reported in the literature.

  3. SYNTHESIS, CRYSTAL AND MOLECULAR-STRUCTURES, UV-VIS SPECTROSCOPY AND ELECTROCHEMICAL PROPERTIES OF 2 IRON(III) PHENOLATE COMPLEXES

    NARCIS (Netherlands)

    LUBBEN, M; MEETSMA, A; VANBOLHUIS, F; FERINGA, BL; HAGE, R

    1994-01-01

    The synthesis and molecular structures of two iron(III) phenolate complexes [(L(1))FeCl] (1) and [(L(2))(2)Fe][BPh(4)] (2) are described, where L(1)H(2) is 2,3-dimethyl-2,3-bis(3-tert-butylsalicylideneamino) butane and L(2)H is 2-(2-pyridyl)-1-salicylideneaminoethane. The complexes have been charact

  4. Elevated levels of circulating IL-7 and IL-15 in patients with early stage prostate cancer

    Directory of Open Access Journals (Sweden)

    Bachmann Alexander

    2011-09-01

    Full Text Available Abstract Background Chronic inflammation has been suggested to favour prostate cancer (PCA development. Interleukins (IL represent essential inflammation mediators. IL-2, IL-7, IL-15 and IL-21, sharing a common receptor γ chain (c-γ, control T lymphocyte homeostasis and proliferation and play major roles in regulating cancer-immune system interactions. We evaluated local IL-2, IL-7, IL-15 and IL-21 gene expression in prostate tissues from patients with early stage PCA or benign prostatic hyperplasia (BPH. As control, we used IL-6 gene, encoding an IL involved in PCA progression. IL-6, IL-7 and IL-15 titres were also measured in patients' sera. Methods Eighty patients with BPH and 79 with early (1 to 2c stage PCA were enrolled. Gene expression in prostate tissues was analyzed by quantitative real-time PCR (qRT-PCR. Serum IL concentrations and acute phase protein titres were evaluated by ELISA. Mann-Whitney, Wilcoxon and χ2 tests were used to compare IL gene expression and serum titers in the two groups of patients. Receiver operating characteristic (ROC curves were constructed to evaluate the possibility to distinguish sera from different groups of patients based on IL titers. Results IL-2 and IL-21 gene expression was comparably detectable, with low frequency and at low extents, in PCA and BPH tissues. In contrast, IL-6, IL-7 and IL-15 genes were expressed more frequently (p Conclusions Expression IL-7 and IL-15 genes in prostate tissues and corresponding serum titres are significantly increased in patients with early stage PCA as compared with patients with BPH.

  5. Role of androgen receptor in prostatic neoplasia versus hyperplasia

    Directory of Open Access Journals (Sweden)

    Irma Husain

    2016-01-01

    Discussion: AR nuclear expression is present in benign and malignant prostatic epithelium. In this study, cases of prostate cancer demonstrated a higher staining intensity for AR when compared with BPH. The intensity of AR staining in prostate cancer significantly reduces as the Gleason grade of the tumor increases. The staining intensity for AR was heterogeneous specifically in cases of prostate cancer. Our results indicate that AR maybe considered as a prognostic marker in prostate cancer.

  6. Development of a Hybrid Optical Biopsy Probe to Improve Prostate Cancer Diagnosis

    Science.gov (United States)

    2011-06-01

    taken on 3 identified regions for cancer, normal and BPH . The measured tissues will be then sliced out and sent to pathology for analysis. Blue and...cancer volumes (assessed by % involvement of the biopsy cores). Fresh prostatectomy sample will be brought to the pathology room immersed in saline...prostate surface as per the regular pathology protocol (blue dye for left side, orange dye for right side, green dye for anterior margin). (2) Prostate

  7. Evaluation of the resistive index of prostatic blood flow in benign prostatic hyperplasia

    Directory of Open Access Journals (Sweden)

    Osama Abdelwahab

    2012-04-01

    Full Text Available OBJECTIVE: The aim of this work is to study the resistive index (RI of prostatic blood flow by transrectal power Doppler sonography in benign prostatic hyperplasia (BPH to determine its correlation with other parameters of BPH. MATERIALS AND METHODS: Eighty-two male patients aged 52-86 years with lower urinary tract symptoms (LUTS due to BPH were included in the study. Patients with prostate cancer, neurogenic bladder, or with other pathology (e.g. prostatitis, bladder stone were excluded from the study. All patients were evaluated by full history including Internatinoal Prostate Symptoms Score (IPSS, general and local examination (DRE, neurologic examination, uroflowmetry, laboratory investigations including urine analysis, routine laboratory tests and serum prostate specific antigen (PSA. Transrectal ultrasonography was used to calculate the total prostatic volume. Transrectal Power Doppler Ultrasound (PUD was used to identify the capsular and urethral arteries of the prostate and to measures the RI value. RESULTS: The mean prostate volume was 75.1 ± 44.7 g. The mean RI of the right and left capsular arteries were 0.76 ± 0.06 and 0.76 ± 0.07, respectively. The mean RI of the urethral arteries was 0.76 ± 0.08. There was a high significative correlation between the increase of the RI of the right and left capsular and urethral arteries and the degree of obstruction (P value < 0.001, severity of symptoms (P value < 0.001 and also the prostatic volume (P value < 0.001. CONCLUSION: Resistive index of the prostatic blood flow can be applied as an easy and non-invasive tool to evaluate the lower urinary tract obstruction due to BPH.

  8. 2011 Aerospace Medical Certification Statistical Handbook

    Science.gov (United States)

    2013-01-01

    BMI Category 38 Table 21. Top 15 Reported Medical Conditions Pathology Description Number Percent Hypertension with medication 65,902 11.08...benign prostatic hypertrophy ( BPH ) 22,558 3.79 Other esophagus, stomach, and duodenal conditions – severe gastritis, esophageal reflux, achalasia, GERD...depersonalization, hypochondriacal, adjustment disorder and other neurosis 11,171 1.88 Other general heart pathology (abnormal ECG, open heart

  9. 2010 Aerospace Medical Certification Statistical Handbook

    Science.gov (United States)

    2012-02-01

    Time of Exam 40..... 43 Figure 26. Special Issuance by BMI Category 41 44 Table 21. Top 15 Reported Medical Conditions Pathology Description Number...transurethral resection of prostate (TURP), spermatocele, benign prostatic hypertrophy ( BPH ) 22,827 3.81 Other esophagus, stomach, and duodenal conditions...1.70 Other general heart pathology (abnormal ECG, open heart surgery, etc.). Wolff-Parkinson-White syndrome, ablation, AV block, right bundle

  10. Oxidative stress in prostate hyperplasia and carcinogenesis

    OpenAIRE

    Udensi K. Udensi; Tchounwou, Paul B.

    2016-01-01

    Prostatic hyperplasia (PH) is a common urologic disease that affects mostly elderly men. PH can be classified as benign prostatic hyperplasia (BPH), or prostate cancer (PCa) based on its severity. Oxidative stress (OS) is known to influence the activities of inflammatory mediators and other cellular processes involved in the initiation, promotion and progression of human neoplasms including prostate cancer. Scientific evidence also suggests that micronutrient supplementation may restore the a...

  11. Prostatic diseases under focus in a university hospital in Eastern Saudi Arabia

    OpenAIRE

    Nemer, Areej M Al; Aldamanhori, Reem B.

    2015-01-01

    Objectives: To explore the spectrum of pathologies diagnosed in prostatic biopsies of Saudi men, and test whether the frequency of diagnosing the malignant fraction has been changed over the last 15 years, and assess the association between chronic inflammation (CI) with both benign prostatic hyperplasia (BPH) and cancer (PCa), and investigate the histological findings of cases presented with acute urinary retention (AUR) clinically. Methods: This is a retrospective cohort study including all...

  12. The Role of NFIB in Prostate Cancer Progression

    Science.gov (United States)

    2015-09-01

    Pathology 3-9 Dr. Grabowska Subtask 3: Perform immunohistochemistry for NFIB and have it scored (Dr. Justin Cates, Pathology ) 10-12 Dr. Grabowska...microarray design stage, I worked with the VUMC Translational Pathology Shared Resource Core to obtain an estimate for the cost of generating the...Principal Investigator 5P20 DK097782-02 Hayward (PI) 09/30/12 - 07/31/14 NIH/NIDDK Obesity, Inflammation and BPH This planning center will develop

  13. Impact of the metabolic syndrome on reproductive health in males of infertile couples

    OpenAIRE

    Francesco Lotti

    2014-01-01

    AIM OF THE THESIS Since Metabolic Syndrome (MetS) is essentially based on increased adiposity and it is associated with male hypogonadism, erectile dysfunction, psychological disturbances, and BPH/LUTS, and all these factors might, in different ways, affect reproductive capacity, we investigated their possible correlations with MetS. Hence, we performed two studies. In the first study (study 1) we evaluated possible associations between MetS, semen and hormonal parameters, as well as c...

  14. Risk factors for prostatic inflammation extent and infection in benign prostatic hyperplasia

    Institute of Scientific and Technical Information of China (English)

    Fa-Xian Yi; Qiang Wei; Hong Li; Xiang Li; Ming Shi; Qiang Dong; Yu-Ru Yang

    2006-01-01

    Aim: To investigate the risk factors for prostatic inflammation extent and infection in patients with benign prostatic hyperplasia (BPH) so as to manage prostatic inflammation more efficiently. Methods: Sixty patients with BPH undergoing TURP between September 2005 and December 2005 in West China Hospital of Sichuan University were studied. Prostate fluid (PF) was collected for the measurement of secretory IgA (SIgA) and complement 3 (C3).Prostate tissue were collected for testing bacterial 16S rDNA by real-time PCR, examining SIgA in the tissue and examining the inflammation. The possible clinical and immune risk factors for prostatic inflammation or infection were analyzed by using the logistic regression method. Results: Abnormal white blood cell count in urinalysis, prostatic infection and a high concentration of C3 in PF are the risk factors for prostatic inflammation extent (P = 0.025, 0.034 and 0.035, respectively and odds ratio [OR] = 18.269, 8.284 and 1.508, respectively). Risk factors for prostatic infection include the C3 concentration and the concentration of S IgA in PF (P = 0.003 and 0.013, respectively, and OR= 1.645 and 0.993, respectively). Conclusion: The present study suggests that prostatic inflammation is associated with urinary tract infection, prostatic infection and the activated complement and that prostatic infection is associated with the activated complement and downregulated mucosal immunity in prostates of the patients with BPH. It is also suggested that individual immune regulation should be considered in the treatment of prostatic inflammation and infection of patients with BPH.

  15. EVALUATION OF FREE-TO-TOTAL PROSTATE SPECIFIC ANTIGEN RATIO IN THE DIAGNOSIS OF PROSTATE CANCER

    Institute of Scientific and Technical Information of China (English)

    2000-01-01

    @@ It's reported that free to total prostate specific antigen ration (f/tPSA) can provide more benefit than the single use of prostate specific antigen (PSA) in the diagnosis of prostate cancer (PCa). We measured serum PSA and fPSA levels in 62 cases of benign prostatic hyperplasia (BPH) and 40 cases of PCa using radioimmunoassay, with patients' age range 59y~ 89y.

  16. Benign prostatic hyperplasia: age-related tissue-remodeling.

    Science.gov (United States)

    Untergasser, Gerold; Madersbacher, Stephan; Berger, Peter

    2005-03-01

    Aging and androgens are the two established risk factors for the development of benign prostatic hyperplasia (BPH) and benign prostatic enlargement (BPE), which can lead to lower urinary tract symptoms (LUTS) in elderly men. BPH, consisting of a nodular overgrowth of the epithelium and fibromuscular tissue within transition zone and periurethral areas, is first detectable around the fourth decade of life and affects nearly all men by the ninth decade. The pathogenesis of BPH is still largely unresolved, but multiple partially overlapping and complementary theories have been proposed, all of which seem to be operative at least to some extent. In addition to nerve-, endocrine- and immune system, local para- and luminocrine pleiotrope mechanisms/factors are implicated in the prostatic tissue-remodeling process. Prostate tissue-remodeling in the transition zone is characterized by: (i) hypertrophic basal cells, (ii) altered secretions of luminal cells leading to calcification, clogged ducts and inflammation, (iii) lymphocytic infiltration with production of proinflammatory cytokines, (iv) increased radical oxygen species (ROS) production that damages epithelial and stromal cells, (v) increased basic fibroblast (bFGF) and transforming growth factor beta (TGF-beta 1) production leading to stromal proliferation, transdifferentiation and extracellular matrix production, (vi) altered autonomous innervation that decreases relaxation and leads to a high adrenergic tonus, (vii) and altered neuroendocine cell function and release of neuroendocrine peptides (NEP). This review summarizes the multifactorial nature of prostate tissue remodeling in elderly men with symptomatic BPH with a particular focus on changes of cell-cell interactions and cell functions in the human aging prostate.

  17. Ejaculatory dysfunction in the treatment of lower urinary tract symptoms

    OpenAIRE

    DeLay, Kenneth Jackson; Nutt,Max; McVary, Kevin T.

    2016-01-01

    The link between lower urinary tract symptoms (LUTS) secondary to benign prostatic hyperplasia (BPH) and sexual dysfunction is well established. Sexual dysfunction can encompass both ejaculatory dysfunction (EjD) and erectile dysfunction (ED). Ejaculatory dysfunction can consist of premature ejaculation, delayed ejaculation, retrograde ejaculation, anejaculation, decreased force of ejaculation and pain upon ejaculation. The impact of different medical and surgical therapies on ejaculatory fun...

  18. 5{alpha}-reductase expression by prostate cancer cell lines and benign prostatic hyperplasia in vitro

    Energy Technology Data Exchange (ETDEWEB)

    Smith, C.M.; Masters, J.R.W. [Univ. College of London (United Kingdom)]|[Pfizer Central Research, Kent (United Kingdom); Ballard, S.A.; Worman, N. [Pfizer Central Research, Sandwich (United Kingdom)

    1996-04-01

    5{alpha}-Reductase (5{alpha}R) activity in two human prostate cancer cell lines was compared to that in benign prostatic hyperplasia (BPH) tissue and COS cells transfected with and expressing the human genes for 5{alpha}-reductase type 1 (5{alpha}R1) and type 2 (5{alpha}R2). Comparisons were based on pH profiles and sensitivities to selective inhibitors of 5{alpha}-reductase. In the cancer lines, activity was greatest over the pH range 7-8, compared to a sharp peak of activity between pH 5-5.5 in BPH tissue and COS cells expressing 5{alpha}R2. Finasteride and SKF105,657 were potent inhibitors of 5{alpha}-reductase activity in BPH tissue and COS cells expressing 5{alpha}R2, but weak inhibitors in the cancer lines and in COS cells expressing 5{alpha}R1. In contrast, LTK1 17,026 was a more potent inhibitor of 5{alpha}-reductase activity in the prostate cancer cell lines and in COS cells expressing 5{alpha}R1. These data indicate that human prostate cancer cell lines express 5{alpha}-reductase activity similar to that in COS cells transfected with 5{alpha}R1, but different from that in BPH tissue. This may be a consequence of in vitro culture. Alternatively, it may reflect a change occurring as a result of neoplastic transformation, in which case it will be important to select appropriate inhibitors in the clinic. 29 refs., 3 figs., 2 tabs.

  19. Predictors of Individual Response to Placebo or Tadalafil 5mg among Men with Lower Urinary Tract Symptoms Secondary to Benign Prostatic Hyperplasia: An Integrated Clinical Data Mining Analysis.

    Directory of Open Access Journals (Sweden)

    Ferdinando Fusco

    Full Text Available A significant percentage of patients with lower urinary tract symptoms (LUTS secondary to benign prostatic hyperplasia (BPH achieve clinically meaningful improvement when receiving placebo or tadalafil 5mg once daily. However, individual patient characteristics associated with treatment response are unknown.This integrated clinical data mining analysis was designed to identify factors associated with a clinically meaningful response to placebo or tadalafil 5mg once daily in an individual patient with LUTS-BPH. Analyses were performed on pooled data from four randomized, placebo-controlled, double-blind, clinical studies, including about 1,500 patients, from which 107 baseline characteristics were selected and 8 response criteria. The split set evaluation method (1,000 repeats was used to estimate prediction accuracy, with the database randomly split into training and test subsets. Logistic Regression (LR, Decision Tree (DT, Support Vector Machine (SVM and Random Forest (RF models were then generated on the training subset and used to predict response in the test subset. Prediction models were generated for placebo and tadalafil 5mg once daily Receiver Operating Curve (ROC analysis was used to select optimal prediction models lying on the ROC surface.International Prostate Symptom Score (IPSS baseline group (mild/moderate vs. severe for active treatment and placebo achieved the highest combined sensitivity and specificity of 70% and ~50% for all analyses, respectively. This was below the sensitivity and specificity threshold of 80% that would enable reliable allocation of an individual patient to either the responder or non-responder group.This extensive clinical data mining study in LUTS-BPH did not identify baseline clinical or demographic characteristics that were sufficiently predictive of an individual patient response to placebo or once daily tadalafil 5mg. However, the study reaffirms the efficacy of tadalalfil 5mg once daily in the

  20. Current trends in management of men with lower urinary tract symptoms and benign prostatic hyperplasia.

    Science.gov (United States)

    Holtgrewe, H L

    1998-04-01

    Management of lower urinary tract symptoms (LUTS) and benign prostatic hyperplasia (BPH) has been central to urology for decades. The urologic community has increasingly come to realize that many men with LUTS do not have prostate enlargement and do not need their prostates debulked surgically. Of all the factors that have emerged to alter the trends associated with management of LUTS and BPH, none has had more impact than the advent of medical therapy. The selective, long-acting, alpha1-blocking agents terazosin, doxazosin, and tamsulosin have become most popular because of their specificity in the urinary tract, reduced side effects, and simplicity of dosage. In addition, finasteride, a 5-alpha-reductase inhibitor, was found to be effective in men with prostates of > or = 40 g. Furthermore, the larger the prostate at baseline, the greater the efficacy of finasteride on symptom relief and flow rate improvement. In addition to medical therapy, an array of device therapies has emerged in the management of LUTS and BPH. Laser prostatectomy is the oldest of the device therapies and includes transurethral vaporization of the prostate (VLAP), transurethral evaporation of the prostate (TUEP), and transurethral interstitial laser prostatectomy (TILP). Studies report beneficial outcomes approaching those achieved with transurethral resection of the prostate (TURP) with less morbidity and a shorter hospital stay. Common diseases contribute the most to national healthcare expenditures. The management of LUTS and BPH are such disorders and result in the expenditure of vast healthcare resources worldwide. The surgical strategies have an established record of outcomes documenting their potential for symptom relief and the avoidance of future complications. Medical and device therapies, although currently promising and attractive, therefore must prove comparable durability.

  1. Hetero-bivalent Imaging Agents for Simultaneous Targeting Prostate-Specific Membrane Antigen (PSMA) and Hepsin

    Science.gov (United States)

    2013-09-01

    11, 12) Hepsin is a type II transmembrane serine protease which is preferentially expressed in neoplastic prostate over benign prostate . (13) In...addition, the mRNA level of hepsin was elevated in ~ 90% of PCa specimens and was > 10-fold higher in metastatic PCa than in normal prostate or benign ... prostatic hyperplasia (BPH). Hepsin is composed of 413 amino acids and a large C-terminal residue is located in the extracellular region. The serine

  2. Apoptose, Inflammation und Angiogenese beim Prostatakarzinom und der benignen Prostatahyperplasie

    OpenAIRE

    Niß-Engel, Wiebke

    2010-01-01

    Sowohl die benigne Prostatahyperplasie (BPH) als auch das Prostatakarzinom (PCA) erlangen als Erkrankungen des Mannes zunehmende Bedeutung. Ziel: Untersuchungsergebnisse zu Markierungsverhalten und Verteilungsmustern verschiedener Proteine bei PCAs und BPHs. Resektate von 50 Patienten wurden immunhistochemisch mit Antikörpern gegen COX2, RANTES, TLR2, BCL2, CASP3 und -9, Fasl, MMP und VEGF gefärbt. Sowohl bei den Markern der Apoptose als auch denen der Inflammation zeigte sich ein...

  3. Die transurethrale Elektroresektion der Prostata (TURP)

    OpenAIRE

    Böhm, Jürgen

    2006-01-01

    Aufgrund der Vielfalt neuer Methoden zur Behandlung der benignen Prostatahyperplasie (BPH) wird in dieser Arbeit der Stellenwert der transurethralen Resektion der Prostata (TURP) als Goldstandardtherapie überprüft. Diesbezüglich wurden die relevanten Daten (insbesondere in Hinblick auf die Langzeitergebnisse) von über 1000 Patienten erhoben, die sich zwischen 1989 und 1997 einer TURP in der urologischen Klinik und Poliklinik (Klinikum rechts der Isar, München) unterzogen hatten. Zur weiteren ...

  4. eine randomisierte Studie an 200 Patienten mit Prostatae kleiner 100 g und urodynamisch nachgewiesener Obstruktion

    OpenAIRE

    Ahyai, Sascha A.

    2006-01-01

    Ziele: Trotz ihrer signifikanten Morbidität stellt die transurethrale Resektion der Prostata (TURP) den gold standard dar für die instrumentelle Therapie der durch die benigne Prostatahyperplasie (BPH) bedingten Blasenauslassobstruktion. Mit dem Hochleistungs-Holmium: YAG Laser kann endoskopisch, in einer relativ blutungsarmen Weise, Prostatagewebe enukleiert werden. Wir verglichen die Technik der transurethralen Holmium-Laser-Enukleation der Prostata (HoLEP) mit der Standard-TURP bei der ope...

  5. Endokrinologie der Benignen Prostatahyperplasie

    OpenAIRE

    Rumpold H; Berger P.; Madersbacher S

    2002-01-01

    Die Prostata ist ein endokrines Zielorgan, für dessen Entwicklung, Funktion und Wachstum Androgene unabdingbar sind. Sie atrophiert nach Kastration und erreicht nach Androgensubstitution wieder ihre normale Funktion. Paradoxerweise entwickeln sich benigne Prostatahyperplasie (BPH) und benigne Prostatavergrößerung (BPE) in einem Lebensabschnitt, der durch einen langsamen Abfall der Serumandrogene gekennzeichnet ist, sodaß neben Androgenen weitere Wachstumsregulatoren an deren Entstehung mitbet...

  6. Genome-wide mapping of virulence in brown planthopper identifies loci that break down host plant resistance.

    Science.gov (United States)

    Jing, Shengli; Zhang, Lei; Ma, Yinhua; Liu, Bingfang; Zhao, Yan; Yu, Hangjin; Zhou, Xi; Qin, Rui; Zhu, Lili; He, Guangcun

    2014-01-01

    Insects and plants have coexisted for over 350 million years and their interactions have affected ecosystems and agricultural practices worldwide. Variation in herbivorous insects' virulence to circumvent host resistance has been extensively documented. However, despite decades of investigation, the genetic foundations of virulence are currently unknown. The brown planthopper (Nilaparvata lugens) is the most destructive rice (Oryza sativa) pest in the world. The identification of the resistance gene Bph1 and its introduction in commercial rice varieties prompted the emergence of a new virulent brown planthopper biotype that was able to break the resistance conferred by Bph1. In this study, we aimed to construct a high density linkage map for the brown planthopper and identify the loci responsible for its virulence in order to determine their genetic architecture. Based on genotyping data for hundreds of molecular markers in three mapping populations, we constructed the most comprehensive linkage map available for this species, covering 96.6% of its genome. Fifteen chromosomes were anchored with 124 gene-specific markers. Using genome-wide scanning and interval mapping, the Qhp7 locus that governs preference for Bph1 plants was mapped to a 0.1 cM region of chromosome 7. In addition, two major QTLs that govern the rate of insect growth on resistant rice plants were identified on chromosomes 5 (Qgr5) and 14 (Qgr14). This is the first study to successfully locate virulence in the genome of this important agricultural insect by marker-based genetic mapping. Our results show that the virulence which overcomes the resistance conferred by Bph1 is controlled by a few major genes and that the components of virulence originate from independent genetic characters. The isolation of these loci will enable the elucidation of the molecular mechanisms underpinning the rice-brown planthopper interaction and facilitate the development of durable approaches for controlling this most

  7. The Concise Guide to PHARMACOLOGY 2013/14: overview.

    OpenAIRE

    2013-01-01

    The Concise Guide to PHARMACOLOGY 2013/14 provides concise overviews of the key properties of over 2000 human drug targets with their pharmacology, plus links to an open access knowledgebase of drug targets and their ligands (www.guidetopharmacology.org), which provides more detailed views of target and ligand properties from the IUPHAR database. The full contents can be found at http://onlinelibrary.wiley.com/doi/10.1111/bph.12444/full. This compilation of the major pharmacological targets i...

  8. Plasma-derived exosomal survivin, a plausible biomarker for early detection of prostate cancer.

    Directory of Open Access Journals (Sweden)

    Salma Khan

    Full Text Available BACKGROUND: Survivin is expressed in prostate cancer (PCa, and its downregulation sensitizes PCa cells to chemotherapeutic agents in vitro and in vivo. Small membrane-bound vesicles called exosomes, secreted from the endosomal membrane compartment, contain RNA and protein that they readily transport via exosome internalization into recipient cells. Recent progress has shown that tumor-derived exosomes play multiple roles in tumor growth and metastasis and may produce these functions via immune escape, tumor invasion and angiogenesis. Furthermore, exosome analysis may provide novel biomarkers to diagnose or monitor PCa treatment. METHODS: Exosomes were purified from the plasma and serum from 39 PCa patients, 20 BPH patients, 8 prostate cancer recurrent and 16 healthy controls using ultracentrifugation and their quantities and qualities were quantified and visualized from both the plasma and the purified exosomes using ELISA and Western blotting, respectively. RESULTS: Survivin was significantly increased in the tumor-derived samples, compared to those from BPH and controls with virtually no difference in the quantity of Survivin detected in exosomes collected from newly diagnosed patients exhibiting low (six or high (nine Gleason scores. Exosome Survivin levels were also higher in patients that had relapsed on chemotherapy compared to controls. CONCLUSIONS: These studies demonstrate that Survivin exists in plasma exosomes from both normal, BPH and PCa subjects. The relative amounts of exosomal Survivin in PCa plasma was significantly higher than in those with pre-inflammatory BPH and control plasma. This differential expression of exosomal Survivin was seen with both newly diagnosed and advanced PCa subjects with high or low-grade cancers. Analysis of plasma exosomal Survivin levels may offer a convenient tool for diagnosing or monitoring PCa and may, as it is elevated in low as well as high Gleason scored samples, be used for early detection.

  9. Electronic Spectroscopy of B Atoms and B2 Molecules Isolated in Para-H2, Normal-D2, Ne, Ar, Kr, and Xe Matrices

    Science.gov (United States)

    2007-11-02

    permitted by our apparatus, we calibrate all the spectra to the vacuum wavelengths of the Hg lamp calibration lines. We apply a cubic polynomial fit...solids, we determine the thickness from the intensities of the condensed-phase induced IR absorptions of the pH2 molecules themselves. We use an excimer ...atom emission intensity after just a few hundred excitation laser pulses ; figure 10 depicts the accompanying photobleaching of the B/pH2 absorptions

  10. Relationship between lower urinary tract symptoms and objective measures of benign prostatic hyperplasia: a Chinese survey

    Institute of Scientific and Technical Information of China (English)

    WANG Jian-ye; PANG Jian; LIU Ming; ZHANG Yao-guang; ZENG Ping; DING Qiang; HUANG Jian; HE Da-lin; SONG Bo; KONG Chui-ze

    2008-01-01

    Background The enlarged prostate leads to obstruction and lower urinary tract symptoms (LUTS), which comprise frequency, urgency, weak stream, straining and nocturia. This study was conducted in a large series of patients to evaluate the relationship between LUTS as stipulated in the International Prostate Symptom Score (IPSS) and the objective parameters related to benign prostatic hyperplasia (BPH).Methods We enrolled 1295 BPH patients from seven centers. The patients were either at first diagnosis of BPH or had discontinued medical treatment for at least 3 months. Those with several other diseases that may be potential risk factors affecting urinary symptoms were excluded from the study. Age, IPSS, prostate volume, peak flow rate, urine volume and post-voiding residual urine volume were measured. The relationship between IPSS and objective parameters were quantified by means of Spearman correlation coefficients. The differences in these parameters between the groups with mild, moderate or severe symptoms were also evaluated.Results Statistically significant correlations were found between IPSS and objective parameters by means of Spearman correlation coefficients. When the patients were divided into three groups with different severities of symptoms,there were significant differences in peak flow rate, urine volume, prostate volume, residue urine volume and quality of life, whereas average age and prostate-specific antigen levels were similar. However, there was evident overlap of these parameters between the groups. The same results were found when the irritative or obstructive subscore of IPSS was considered.Conclusions The correlation between objective parameters of BPH and LUTS is significant. However, it is hard to predict the severity of symptoms by these parameters.

  11. The relationship between angiogenesis and cyclooxygenase-2 expression in prostate cancer

    OpenAIRE

    Mukherjee, R.; Edwards, J; Underwood, M.A.; Bartlett, J M S

    2005-01-01

    OBJECTIVE: To test the hypothesis that angiogenesis in prostate cancer is associated with tumour invasion and metastasis, and that this is mediated through increased cyclooxygenase-2 (COX-2) expression.\\ud \\ud PATIENTS AND METHODS: Angiogenesis was assessed in 105 patients with either prostate cancer (79) or benign prostatic hyperplasia (BPH, 26) and these data correlated with levels of COX-2 expression in the same dataset. The mean microvessel density (MVD) was analysed as a marker of angiog...

  12. silencing COI1 in rice increases susceptibility to chewing insects and impairs inducible defense.

    Directory of Open Access Journals (Sweden)

    Mao Ye

    Full Text Available The jasmonic acid (JA pathway plays a key role in plant defense responses against herbivorous insects. CORONATINE INSENSITIVE1 (COI1 is an F-box protein essential for all jasmonate responses. However, the precise defense function of COI1 in monocotyledonous plants, especially in rice (Oryza sativa L. is largely unknown. We silenced OsCOI1 in rice plants via RNA interference (RNAi to determine the role of OsCOI1 in rice defense against rice leaf folder (LF Cnaphalocrocis medinalis, a chewing insect, and brown planthopper (BPH Nilaparvata lugens, a phloem-feeding insect. In wild-type rice plants (WT, the transcripts of OsCOI1 were strongly and continuously up-regulated by LF infestation and methyl jasmonate (MeJA treatment, but not by BPH infestation. The abundance of trypsin protease inhibitor (TrypPI, and the enzymatic activities of polyphenol oxidase (PPO and peroxidase (POD were enhanced in response to both LF and BPH infestation, but the activity of lipoxygenase (LOX was only induced by LF. The RNAi lines with repressed expression of OsCOI1 showed reduced resistance against LF, but no change against BPH. Silencing OsCOI1 did not alter LF-induced LOX activity and JA content, but it led to a reduction in the TrypPI content, POD and PPO activity by 62.3%, 48.5% and 27.2%, respectively. In addition, MeJA-induced TrypPI and POD activity were reduced by 57.2% and 48.2% in OsCOI1 RNAi plants. These results suggest that OsCOI1 is an indispensable signaling component, controlling JA-regulated defense against chewing insect (LF in rice plants, and COI1 is also required for induction of TrypPI, POD and PPO in rice defense response to LF infestation.

  13. Interspecifc hybrid plants obtained from cultivars (AA) crossed with wild Oryza species (CC)

    Institute of Scientific and Technical Information of China (English)

    TANGShengxiang; YANHuthuang; HuHulying

    1994-01-01

    Wild dee O.officinalis(CNW 258, CNW 259) and O. eichingeri (CNW 260, CNW 261) have CC genome and high resistance to brown planthopper (BPH) and whitebacked planthopper (WBPH). To transfer the resistant gene(s) of the wild rice into Asian cultivated rice, the interspecific crosses between O. sativa L. (AA) and O. officinafis and O.eiclungeri (CC) was made in present study.

  14. Elucidating the Role of Translocator Protein in Prostate Cancer

    Science.gov (United States)

    2009-09-01

    4.8/6) compared to normal donor (2.0/6), normal tissue adjacent to tumor (2.1/6), and benign prostatic hyperplasia (1.8/6). Furthermore, TSPO...immunohistochemistry (IHC) in normal donor prostate (donor), normal prostate tissue adjacent to tumor (NAT), benign prostatic hyperplasia (BPH...benzodiazepine receptor in human endometrium and endometrial carcinoma. Anticancer Research, 2000. 20(1A): p. 463-6. 8. Han, Z., et al., Expression of

  15. Mechanistic insights into acetophenone transfer hydrogenation catalyzed by half-sandwich ruthenium(II) complexes containing 2-(diphenylphosphanyl)aniline - a combined experimental and theoretical study

    NARCIS (Netherlands)

    A. Bacchi; M. Balordi; R. Cammi; L. Elviri; C. Pelizzi; F. Picchioni; V. Verdolino; K. Goubitz; R. Peschar; P. Pelagatti

    2008-01-01

    Several new half-sandwich ruthenium(II) complexes containing 2-(diphenyphosphanyl)aniline (PNH2) of formula {Ru[(kappa P-2,N)PNH2](p-cymene)Cl}Y [Y = Cl (1a), PF6 (1b), BF4 (1c), BPh4 (1d), TfO (1e)] were synthesized and fully characterized both in solution (H-1 NMR and P-31{H-1) NMR spectroscopy) a

  16. The histology of prostate tissue following prostatic artery embolization for the treatment of benign prostatic hyperplasia

    Directory of Open Access Journals (Sweden)

    George Camara-Lopes

    2013-04-01

    Full Text Available Objective Prostatic artery embolization (PAE for the treatment of patients with symptomatic benign prostatic hyperplasia (BPH is believed to be a safe procedure with a low risk of adverse side effects. Artery embolization is a viable treatment option in patients who are refractory to the classic noninvasive treatments. Knowledge of the histological characteristics of prostate tissue following the procedure is still limited. In this study, we describe the microscopic aspects of the prostate following PAE for BPH. Materials and Methods Two patients underwent transurethral resections of the prostate (TURP after PAE. Embolizations were performed under local anesthesia with an initial pelvic angiography to evaluate the iliac vessels and the prostate arteries using a 2.8 French microcatheter. The prostate was embolized with 300-500µm Microspheres (Embosphere®, using complete blood stasis as the end point. The prostate tissues were analyzed histologically to characterize the effects of the embolization. Results The embolic material within the prostate tissue was easily identified as homogeneous, bright eosin-red spheroids filling the vessel lumens. Ischemic necrosis surrounded or not by chronic inflammatory reactions containing macrophages were considered as a result of the artery embolization. Also, some aspects related to the healing process were observed being fibrotic nodules surrounded by glands with squamous metaplasia of the epithelial lining the most important. In the remaining sections, due to the precocious surgical intervention, the classic findings of BPH were still present with the glandular and stromal hyperplasia associated with nonspecific chronic prostatitis. Conclusions This is the first description of prostate histology in BPH patients treated by PAE, a new procedure that is being used increasingly as a therapeutic intervention. The recognition of the changes caused by this new modality of treatment has become a very important

  17. A case of giant prostatic hyperplasia

    Directory of Open Access Journals (Sweden)

    Luke Wang

    2016-01-01

    Full Text Available Benign prostatic hyperplasia (BPH is one of the most common conditions experienced by aging males and a frequent cause of bladder outlet obstruction and macroscopic haematuria. Giant prostatic hyperplasia (GPH is an extremely rare form of prostatic hyperplasia. We present a case of a patient with GPH of 800 mL. To our knowledge, this is the fourth largest prostatic hyperplasia ever reported in the literature.

  18. Relationship between serum sex hormones levels and degree of benign prostate hyperplasia in Chinese aging men

    Institute of Scientific and Technical Information of China (English)

    Qin-Song Zeng; Zheng Li; Bo Wang; Ying-Hao Sun; Chuan-Liang Xu; Zhi-Yong Liu; Hui-Qing Wang; Bo Yang; Wei-Dong Xu; Tai-Le Jin; Cheng-Yao Wu; Gang Huang

    2012-01-01

    Benign prostatic hyperplasia (BPH) is one of the most common medical conditions in middle aged and older men.This study investigated the relationship between serum levels of sex hormones and measures of BPH in the aging male population of China.Prostate symptoms were assessed as part of a free health screening program for men ≥40 years of age.The examination included digital rectal examination,determination of serum prostate-specific antigen levels,International Prostate Symptom Scorn (IPSS) and transrectal ultrasonography.Serum levels of total testosterone (TT),sex hormone binding globulin (SHBG),free testosterone (FT),luteinizing hormone (LH),follicle-stimulating hormone (FSH),prolactin (PRL) and estradiol (E2) were evaluated.The men also completed a health and demographics questionnaire and received a detailed physical examination.The final study population consisted of 949 men with a mean age of 58.9 years.Pearson correlation analysis indicated that there were significant correlations between age and levels of all sex hormones except TT,and between age and prostate volume (PV; r=-0.243; P<0.01) or IPSS (r=0.263; P<0.01 ).Additional significant correlations were found between IPSS and serum levels of LH (r=0.112; P<0.01 ) and FSH (r=0.074; P<0.05),but there were no significant correlations between sex hormone levels and PV.Multivariate linear regression analysis showed significant correlations between age and body mass index (BMI) with PV (P<0.0001).In addition,there was a significant correlation between age and PV with IPSS (P<0.0001).Serum sex hormone levels did not correlate with PV or IPSS.The effects of endocrine changes on measures of BPH in aging men require further investigation in longitudinal and multicenter studies that include patients with all severities of BPH.

  19. [Zinc, calcium and sodium values in secretions of patients with benign prostatic hyperplasia].

    Science.gov (United States)

    Romics, I; Bach, D

    1990-12-01

    In two randomized patients groups suffering from benign prostatic hyperplasia (BPH) two exprimate samples were drawn at a seven-day interval. In the untreated control group no changes in the values of Ca, Na and Zn were found. In the other group the patients have been treated with ERU capsules (Radicis urticae) for 7 days and thereafter a significant decrease of Zn values were found.

  20. Estimation of clinically significant prostate volumes by digital rectal examination: a comparative prospective study.

    LENUS (Irish Health Repository)

    Ahmad, Sarfraz

    2011-12-01

    Reliable quantification of prostate volume is important to correctly select patients with benign prostatic hyperplasia (BPH) most likely to benefit from medical therapy [e.g. 5 alpha-reductase inhibitors (5-ARIs)] and in selecting appropriate surgical approach. We aim to determine the reliability of digital rectal examination (DRE) in estimation of prostate volume which may be helpful in patient selection for 5-ARIs therapy.