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Sample records for bp950005-4 amplicor roche

  1. Cytomegalovirus quantification in plasma with Abbott RealTime CMV and Roche Cobas Amplicor CMV assays.

    Science.gov (United States)

    Tremblay, Maxime-Antoine; Rodrigue, Marc-André; Deschênes, Louise; Boivin, Guy; Longtin, Jean

    2015-12-01

    We assessed the performance of Abbott RealTime CMV assay (ARC) compared to Roche Cobas Amplicor CMV Monitor Test (RCM) for quantification of CMV in plasma of transplant patients. Commercial panels were used to test linearity, precision and interference and 83 clinical samples were used for the accuracy and precision analyses. All 43 RCM-positive clinical samples tested positive by ARC. The overall concordance between the two tests was good (98%). Based on 17 samples, the inter-assay median coefficient of variation was 13%. A linearity panel ranging from approximately 1 to 7log10copies/mL was used to confirm linearity (R(2)=0.99). CMV viral load measurement was not affected by different concentrations of HSV-1 or EBV DNA. We conclude that The Abbott RealTime CMV assay offers good sensitivity, precision and linearity and is suitable for monitoring CMV viral loads in transplant recipients. Standardization with the WHO CMV standard allows for comparison with other assays. PMID:26341060

  2. Comparison of the Abbott m2000 HIV-1 Real-Time and Roche AMPLICOR Monitor v1.5 HIV-1 assays on plasma specimens from Rakai, Uganda

    OpenAIRE

    Ssebugenyi, I.; Kizza, A; Mpoza, B; Aluma, G.; Boaz, I.; Newell, K.; Laeyendecker, O; Shott, J P; Serwadda, D; Reynolds, S.J.

    2011-01-01

    The need for viral load (VL) monitoring of HIV patients receiving antiretroviral therapy (ART) in resource-limited settings (RLS) has become apparent with studies showing the limitations of immunological monitoring. We compared the Abbott m2000 Real-Time (Abbott) HIV-1 assay with the Roche AMPLICOR Monitor v1.5 (Roche) HIV-1 assay over a range of VL concentrations. Three hundred and eleven plasma samples were tested, including 164 samples from patients on ART ≥ six months and 147 from ART-naï...

  3. Evaluation of the NucliSens EasyQ v2.0 assay in comparison with the Roche Amplicor v1.5 and the Roche CAP/CTM HIV-1 Test v2.0 in quantification of C-clade HIV-1 in plasma.

    Directory of Open Access Journals (Sweden)

    Maximilian Muenchhoff

    Full Text Available Human immunodeficiency virus type 1 (HIV-1 genetic diversity poses a challenge to reliable viral load monitoring. Discrepancies between different testing platforms have been observed, especially for non-clade-B virus. Therefore we compare, in antiretroviral therapy (ART-naïve South African subjects predominantly infected with HIV-1 clade-C, three commercially available assays: the COBAS AmpliPrep/COBAS TaqMan HIV-1 Test version 2.0 by Roche (CAP/CTM v2.0, the BioMérieux NucliSens Version 2.0 Easy Q/Easy Mag (NucliSens v2.0 and the Roche COBAS Amplicor HIV-1 Monitor Test Version 1.5 (Amplicor v1.5. Strong linear correlation was observed and Bland-Altman analyses showed overall good agreement between the assays with mean viral load differences of 0.078 log cp/ml (NucliSens v2.0 - Amplicor v1.5, 0.260 log cp/ml (CAP/CTM v2.0 - Amplicor v1.5 and 0.164 log cp/ml (CAP/CTM v2.0 - NucliSens v2.0, indicating lower mean viral load results for the Amplicor v1.5 and higher mean readings for the CAP/CTM v2.0. Consistent with observations following previous comparisons of CAP/CTM v2.0 versus Amplicor v1.5, the CAP/CTM v2.0 assay detected low-level viremia (median 65 cp/ml in more than one-third of those in whom viremia had been undetectable (<20 cp/ml in assays using the NucliSens platform. These levels of viremia are of uncertain clinical significance but may be of importance in early detection of ART resistance in those on treatment. Overall the three assays showed good comparability of results but with consistent, albeit relatively small, discrepancies for HIV-1 clade-C samples, especially in the low-viremic range that should be taken into account when interpreting viral load data.

  4. Multicenter comparison of Roche COBAS AMPLICOR MONITOR version 1.5, Organon Teknika NucliSens QT with Extractor, and Bayer Quantiplex version 3.0 for quantification of human immunodeficiency virus type 1 RNA in plasma.

    Science.gov (United States)

    Murphy, D G; Côté, L; Fauvel, M; René, P; Vincelette, J

    2000-11-01

    The performance and characteristics of Roche COBAS AMPLICOR HIV-1 MONITOR version 1.5 (CA MONITOR 1.5) UltraSensitive (usCA MONITOR 1. 5) and Standard (stCA MONITOR 1.5) procedures, Organon Teknika NucliSens HIV-1 RNA QT with Extractor (NucliSens), and Bayer Quantiplex HIV RNA version 3.0 (bDNA 3.0) were compared in a multicenter trial. Samples used in this study included 460 plasma specimens from human immunodeficiency virus (HIV) type 1 (HIV-1)-infected persons, 100 plasma specimens from HIV antibody (anti-HIV)-negative persons, and culture supernatants of HIV-1 subtype A to E isolates diluted in anti-HIV-negative plasma. Overall, bDNA 3.0 showed the least variation in RNA measures upon repeat testing. For the Roche assays, usCA MONITOR 1.5 displayed less variation in RNA measures than stCA MONITOR 1.5. NucliSens, at an input volume of 2 ml, showed the best sensitivity. Deming regression analysis indicated that the results of all three assays were significantly correlated (P < 0.0001). However, the mean difference in values between CA MONITOR 1.5 and bDNA 3.0 (0.274 log(10) RNA copies/ml; 95% confidence interval, 0.192 to 0.356) was significantly different from 0, indicating that CA MONITOR 1.5 values were regularly higher than bDNA 3.0 values. Upon testing of 100 anti-HIV-negative plasma specimens, usCA MONITOR 1.5 and NucliSens displayed 100% specificity, while bDNA 3.0 showed 98% specificity. NucliSens quantified 2 of 10 non-subtype B viral isolates at 1 log(10) lower than both CA MONITOR 1.5 and bDNA 3.0. For NucliSens, testing of specimens with greater than 1,000 RNA copies/ml at input volumes of 0.1, 0.2, and 2.0 ml did not affect the quality of results. Additional factors differing between assays included specimen throughput and volume requirements, limit of detection, ease of execution, instrument work space, and costs of disposal. These characteristics, along with assay performance, should be considered when one is selecting a viral load assay.

  5. Comparison of the Abbott m2000 RealTime CT Assay and the Cepheid GeneXpert CT/NG Assay to the Roche Amplicor CT Assay for Detection of Chlamydia trachomatis in Ocular Samples from Tanzania

    OpenAIRE

    Dize, Laura; West, Sheila; Williams, James A; Van Der Pol, Barbara; Quinn, Thomas C.; Gaydos, Charlotte A.

    2013-01-01

    The GeneXpert CT/NG assay (GeneXpert) and the Abbott m2000 RealTime CT (m2000) assay were compared to Amplicor for detecting ocular Chlamydia trachomatis. Discordant specimens were tested by the Aptima CT assay. The m2000 assay sensitivity was 100% (95% confidence interval [CI], 90% to 100%), and specificity was 98.46% (95% CI, 95.2% to 99.2%); GeneXpert sensitivity was 100% (95% CI, 90% to 100%), and specificity was 100% (95% CI, 98.1% to 100%). The m2000 and GeneXpert assays appear to perfo...

  6. Comparison of the COBAS AMPLICOR MTB and BDProbeTec ET assays for detection of Mycobacterium tuberculosis in respiratory specimens.

    NARCIS (Netherlands)

    W.H.F. Goessens (Wil); P. de Man (Peter); J.G. Koeleman; A. Luijendijk (Ad); R. te Witt (René); H.P. Endtz (Hubert); A.F. van Belkum (Alex)

    2005-01-01

    textabstractThe performances of the BDProbeTec ET (Becton Dickinson) and COBAS AMPLICOR MTB (Roche) were retrospectively evaluated for detecting Mycobacterium tuberculosis complex in various respiratory specimens. The BACTEC and MGIT liquid culture system (Becton Dickinson) was used as a reference m

  7. Meeting C.Roche

    CERN Multimedia

    Roche,C

    1986-01-01

    Exposé de Christian Roche suite à un certain nombre d'évémements survenus depuis septembre 1985 concernant la restructuration de l'administration qui ont abouti à une décision prise par la direction

  8. Homage to Jean Roche

    CERN Multimedia

    2010-01-01

    It was with deep sadness that we learnt that Jean Roche had died. Jean was one of those quietly efficient and conscientious pillars of our community who beaver away unobtrusively for decades and make such a contribution to building CERN. An expert in thermal engineering, he left his mark on many buildings on the Meyrin and Prévessin sites. At the time of the LEP project at the beginning of the 1980s he was in charge of defining the principles of the ventilation systems for the new accelerator. Through his design studies and the calls for tenders that he launched, he developed these high-performance state-of-the-art installations which not only made LEP’s successes possible but also served as benchmarks for the fundamental principles underpinning the design of the LHC ventilation system. Jean always liked to surround himself with young people and during the years of his stewardship the design office was a splendid training ground. Despite his responsibilities, Jean was always keen t...

  9. A Java Interface for Roche Lobe Calculations

    Science.gov (United States)

    Leahy, D. A.; Leahy, J. C.

    2015-09-01

    A JAVA interface for calculating various properties of the Roche lobe has been created. The geometry of the Roche lobe is important for studying interacting binary stars, particularly those with compact objects which have a companion which fills the Roche lobe. There is no known analytic solution to the Roche lobe problem. Here the geometry of the Roche lobe is calculated numerically to high accuracy and made available to the user for arbitrary input mass ratio, q.

  10. Monomials, Binomials, and Riemann-Roch

    OpenAIRE

    Manjunath, Madhusudan; Sturmfels, Bernd

    2012-01-01

    The Riemann-Roch theorem on a graph G is related to Alexander duality in combinatorial commutive algebra. We study the lattice ideal given by chip firing on G and the initial ideal whose standard monomials are the G-parking functions. When G is a saturated graph, these ideals are generic and the Scarf complex is a minimal free resolution. Otherwise, syzygies are obtained by degeneration. We also develop a self-contained Riemann-Roch theory for artinian monomial ideals.

  11. Comparison of the COBAS/Ampliprep Taqman and Amplicor HIV-1 monitor tests in Lagos, Nigeria

    Directory of Open Access Journals (Sweden)

    Oluemi S. Amoo

    2013-03-01

    Full Text Available Background: The use of real-time Polymerase chain reaction (PCR technology options is increasing in resource-limited settings because they are faster, improve assay sensitivity,have higher throughput, larger dynamic ranges and reduced rates of contamination. In 2010, UNAIDS ranked Nigeria as the second highest population of people living with HIV and AIDS (2.98 million people in the world.Objective: The objective of this study was to compare the analytical performances of the Amplicor HIV-1 Monitor (version 1.5 and the COBAS Ampliprep/Taqman (version 2.0 usedin monitoring HIV disease progression in HIV-infected individuals.Method: In a cross-sectional study, HIV-1 RNA values obtained with the Amplicor HIV-1 monitor version 1.5 were compared with those of the COBAS/Ampliprep TaqMan HIV-1version 2.0 in a routine clinical setting. Between May and November 2011, 176 plasma samples collected were analysed in parallel using both techniques. Data analysis was done using statgraphics Centurion XVI and Medcalc version 12.0.Result: The correlation coefficient for the two assays was 0.83 and the level of agreement using a Bland–Altman plot was 94.2%.Conclusion: These findings suggest that the results from the two methods were comparable, hence the COBAS/Ampliprep Taqman version 2.0 is recommended for high-volume laboratories.

  12. Diagnostic value of Cobas Amplicor MTB and Rotorgene Real Time PCR for tuberculous meningitis: A six-year retrospective study

    Directory of Open Access Journals (Sweden)

    Gülnur Tarhan

    2015-12-01

    Full Text Available Objective: Tuberculous meningitis (TBM is the most severe and lethal form of tuberculosis (TB.Bacteriologic confirmation of TBM is difficult and slow. Therefore, most patients receive ntituberculosis treatment based only on clinical and cerebrospinal fluid (CSF characteristics. Rapid diagnosis of TBM is important to decrease morbidity and mortality. The aim of the study was to demonstrate that COBAS Amplicor MTB and Rotorgene Real Time (RT PCR is a rapid method of diagnosing TBM before and after initiating anti-tuberculosis treatment. Methods: A retrospective study was conducted between December 2002 and January 2009 in 468 patients with suspected TBM. Clinical specimens were collected from different hospitals in Ankara. All specimens were evaluated by smear microscopy and culture methods with Lowenstein-Jensen (LJ and MGIT culture system. Results: Using culture results as a gold standard, the sensitivity, specificity, positive predictive values (PPV, and negative predictive values (NPV were 71.0%, 98.8%, 97.8% and 75.0%, respectively, for COBAS Amplicor MTB and 80%, 98.9%, 99.0% and 80.0%, respectively, for Rotorgene RT PCR. Statistical analysis showed no significant differences between the COBAS Amplicor MTB and Rotorgene RT PCR (p≥0.05. All isolates were susceptible to isoniazid, rifampin, streptomycin, and ethambutol with proportion method in LJ medium. All isolates were defined as LAM7-TUR by spoligotyping. Conclusion: Retrospective analysis of COBAS Amplicor MTB and Rotorgene RT PCR found that both tests are effective in rapidly diagnosing MTB using CSF. It was concluded that Rotorgene RT PCR test is more sensitive (81.0% than COBAS Amplicor MTB (71.0%. J Microbiol Infect Dis 2015;5(4: 156-161

  13. Roche volume filling and the dissolution of open star clusters

    CERN Document Server

    Ernst, A; Just, A; Noel, T

    2015-01-01

    From direct N-body simulations we find that the dynamical evolution of star clusters is strongly influenced by the Roche volume filling factor. We present a parameter study of the dissolution of open star clusters with different Roche volume filling factors and different particle numbers. We study both Roche volume underfilling and overfilling models and compare with the Roche volume filling case. We find that in the Roche volume overfilling limit of our simulations two-body relaxation is no longer the dominant dissolution mechanism but the changing cluster potential. We call this mechnism "mass-loss driven dissolution" in contrast to "two-body relaxation driven dissolution" which occurs in the Roche volume underfilling regime. We have measured scaling exponents of the dissolution time with the two-body relaxation time. In this experimental study we find a decreasing scaling exponent with increasing Roche volume filling factor. The evolution of the escaper number in the Roche volume overfilling limit can be d...

  14. Comparison of COBAS AMPLICOR Neissefia gonorrhoeae PCR, including confirmation with N-gonorrhoeae-specific 16S rRNA PCR, with traditional culture

    NARCIS (Netherlands)

    Luijt, DS; Bos, PAJ; van Zwet, AA; Vader, PCV; Schirm, J

    2005-01-01

    A total of 3,023 clinical specimens were tested for Neisseria gonorrhoeae by using COBAS AMPLICOR (CA) PCR and confirmation of positives by N. gonorrhoeae-specific 16S rRNA PCR. The sensitivity of CA plus 16S rRNA PCR was 98.8%, compared to 68.2% for culture. Confirmation of CA positives increased t

  15. Comparison of COBAS AMPLICOR Neisseria gonorrhoeae PCR, including confirmation with N. gonorrhoeae-specific 16S rRNA PCR, with traditional culture

    NARCIS (Netherlands)

    Luijt, D.S.; Bos, P.A.; van Zwet, A.A.; Voorst-Vader, P.C.; Schirm, J.

    2005-01-01

    : A total of 3,023 clinical specimens were tested for Neisseria gonorrhoeae by using COBAS AMPLICOR (CA) PCR and confirmation of positives by N. gonorrhoeae-specific 16S rRNA PCR. The sensitivity of CA plus 16S rRNA PCR was 98.8%, compared to 68.2% for culture. Confirmation of CA positives increased

  16. Binary Evolution: Roche Lobe Overflow and Blue Stragglers

    CERN Document Server

    Ivanova, Natalia

    2014-01-01

    One of the principal mechanisms that is responsible for the origin of blue stragglers is mass transfer that takes place while one of the binary companions overfills its Roche lobe. In this Chapter, we overview the theoretical understanding of mass transfer via Roche lobe overflow: classification, how both the donor and of the accretor respond to the mass transfer on different timescales (adiabatic response, equilibrium response, superadiabatic response, time-dependent response) for different types of their envelopes (convective and radiative). These responses, as well as the assumption on how liberal the process is, are discussed in terms of the stability of the ensuing mass transfer. The predictions of the theory of mass transfer via Roche lobe overflow are then briefly compared with the observed mass-transferring systems with both degenerate and non-degenerate donors. We conclude with the discussion which cases of mass transfer and which primordial binaries could be responsible for blue stragglers formation...

  17. A Riemann-Roch theorem for the noncommutative two torus

    Science.gov (United States)

    Khalkhali, Masoud; Moatadelro, Ali

    2014-12-01

    We prove the analogue of the Riemann-Roch formula for the noncommutative two torus Aθ = C(Tθ2)equipped with an arbitrary translation invariant complex structure and a Weyl factor represented by a positive element k ∈C∞(Tθ2). We consider a topologically trivial line bundle equipped with a general holomorphic structure and the corresponding twisted Dolbeault Laplacians. We define a spectral triple (Aθ , H , D) that encodes the twisted Dolbeault complex of Aθ and whose index gives the left hand side of the Riemann-Roch formula. Using Connes' pseudodifferential calculus and heat equation techniques, we explicitly compute the b2 terms of the asymptotic expansion of Tr(e-tD2) . We find that the curvature term on the right hand side of the Riemann-Roch formula coincides with the scalar curvature of the noncommutative torus recently defined and computed in Connes and Moscovici (2014) and independently computed in Fathizadeh and Khalkhali (2014).

  18. An application of the Riemann-Roch theorem

    Institute of Scientific and Technical Information of China (English)

    2008-01-01

    Applying the Riemann-Roch theorem,we calculate the dimension of a kind of mero- morphicλ-differentials’ space on compact Riemann surfaces.And we also construct a basis of theλ-differentials’ space.As the main result,the Cauchy type of integral formula on compact Riemann surfaces is established.

  19. Comparison of real-time polymerase chain reaction with the COBAS Amplicor test for quantitation of hepatitis B virus DNA in serum samples

    Institute of Scientific and Technical Information of China (English)

    Ming Shi; Yong Zhang; Ying-Hua Zhu; Jing Zhang; Wei-Jia Xu

    2008-01-01

    AIM: To compare the clinical performance of a real-time PCR assay with the COBAS Amplicor Hepatitis B Virus (HBV) Monitor test for quantitation of HBV DNA in serum samples. METHODS: The reference sera of the Chinese National Institute for the Control of Pharmaceutical and Biological Products and the National Center for Clinical Laboratories of China, and 158 clinical serum samples were used in this study. The linearity, accuracy, reproducibility, assay time, and costs of the real-time PCR were evaluated and compared with those of the Cobas Amplicor test. RESULTS: The intra-assay and inter-assay variations of the real-time PCR ranged from 0.3% to 3.8% and 1.4% to 8.1%, respectively. The HBV DNA levels measured by the real-time PCR correlated very well with those obtained with the COBAS Amplicor test (r = 0.948). The real-time PCR HBV DNA kit was much cheaper and had a wider dynamic range. CONCLUSION: The real-time PCR assay is an excellent tool for monitoring of HBV DNA levels in patients with chronic hepatitis B.

  20. ["Wetlands". Charles Bukowski and Charlotte Roche on hemorrhoids].

    Science.gov (United States)

    Bahmer, F A; Bahmer, J A

    2010-08-01

    More than 40 years ago Charles Bukowski described his experiences with coloscopy and the ensuing surgery on his hemorrhoids, both performed by a doctor pictured as sadistic. Bukowski not only depicts these procedures but also characterizes his compassionate inpatients as well as the nursing staff. In her bestseller published in 2008 Charlotte Roche's protagonist Helen needs surgical inpatient treatment because of hemorrhoidal bleeding. Her stay in the hospital, prolonged by a postoperative self-inflicted bleeding, provides the basis not only for strategies to bring her divorced parents together but also for thoughts on genitalia, manifold sexual practices, as well as on rules of hygiene. From a psychodynamic viewpoint the protagonists in both stories suffer from a depressive basic conflict, compensated in Bukowski's work by a dependent, self-destructive, philobatic form of coping and in Roche's alter ego Helen by an impulsive, sexualized behaviour.

  1. Submaximal Riemann-Roch expected curves and symplectic packing.

    Directory of Open Access Journals (Sweden)

    Wioletta Syzdek

    2007-06-01

    Full Text Available We study Riemann-Roch expected curves on $mathbb{P}^1 imes mathbb{P}^1$ in the context of the Nagata-Biran conjecture. This conjecture predicts that for sufficiently large number of points multiple points Seshadri constants of an ample line bundle on algebraic surface are maximal. Biran gives an effective lower bound $N_0$. We construct examples verifying to the effect that the assertions of the Nagata-Biran conjecture can not hold for small number of points. We discuss cases where our construction fails. We observe also that there exists a strong relation between Riemann-Roch expected curves on $mathbb{P}^1 imes mathbb{P}^1$ and the symplectic packing problem. Biran relates the packing problem to the existence of solutions of certain Diophantine equations. We construct such solutions for any ample line bundle on $mathbb{P}^1 imes mathbb{P}^1$ and a relatively smallnumber of points. The solutions geometrically correspond to Riemann-Roch expected curves. Finally we discuss in how far the Biran number $N_0$ is optimal in the case of mathbb{P}^1 imes mathbb{P}^1. In fact we conjecture that it can be replaced by a lower number and we provide evidence justifying this conjecture.

  2. Roche Accretion of stars close to massive black holes

    CERN Document Server

    Lixin,; Blandford, Roger D

    2011-01-01

    In this paper we consider Roche accretion in an Extreme Mass-Ratio Inspiral (EMRI) binary system formed by a star orbiting a massive black hole. The ultimate goal is to detect the mass and spin of the black hole and provide a test of general relativity in the strong-field regime from the resultant quasi-periodic signals. Before accretion starts, the stellar orbit is presumed to be circular and equatorial, and shrinks due to gravitational radiation. New fitting formulae are presented for the inspiral time and the radiation-reaction torque in the relativistic regime. If the inspiralling star fills its Roche lobe outside the Innermost Stable Circular Orbit (ISCO) of the hole, gas will flow through the inner Lagrange point (L1) to the hole. We give new relativistic interpolation formulae for the volume enclosed by the Roche lobe. If this mass-transfer happens on a time scale faster than the thermal time scale but slower than the dynamical time scale, the star will evolve adiabatically, and, in most cases, will re...

  3. Comparison of two commercial assays for detection of human papillomavirus (HPV) in cervical scrape specimens: validation of the Roche AMPLICOR HPV test as a means to screen for HPV genotypes associated with a higher risk of cervical disorders.

    NARCIS (Netherlands)

    Ham, M.A. van; Bakkers, J.M.J.E.; Harbers, G.; Quint, W.G.V.; Massuger, L.F.A.G.; Melchers, W.J.G.

    2005-01-01

    Certain high-risk (HR) human papillomavirus (HPV) types are a necessary cause for the development of cervical disorders. Women with persistent HR HPV infections have an increased risk of developing high-grade cervical lesions, compared with those who have no or low-risk HPV infections. Therefore, im

  4. Mécanique des sols et des roches

    CERN Document Server

    Vullier, Laurent; Zhao, Jian

    2016-01-01

    La mécanique des sols et la mécanique des roches sont des disciplines généralement traitées séparément dans la littérature. Pour la première fois, un traité réunit ces deux spécialités, en intégrant également les connaissances en lien avec les écoulements souterrains et les transferts thermiques. A la fois théorique et pratique, cet ouvrage propose tout d'abord une description détaillée de la nature et de la composition des sols et des roches, puis s'attache à la modélisation de problèmes aux conditions limites et présente les essais permettant de caractériser les sols et les roches, tant d'un point de vue mécanique qu'hydraulique et thermique. La problématique des sols non saturés et des écoulements multiphasiques est également abordée. Une attention particulière est portée aux lois de comportement mécanique et à la détermination de leurs paramètres par des essais in situ et en laboratoire, et l'ouvrage offre également une présentation détaillée des systèmes de classi...

  5. Riemann-Roch Spaces and Linear Network Codes

    DEFF Research Database (Denmark)

    Hansen, Johan P.

    2015-01-01

    We construct linear network codes utilizing algebraic curves over finite fields and certain associated Riemann-Roch spaces and present methods to obtain their parameters. In particular we treat the Hermitian curve and the curves associated with the Suzuki and Ree groups all having the maximal...... number of points for curves of their respective genera. Linear network coding transmits information in terms of a basis of a vector space and the information is received as a basis of a possibly altered vector space. Ralf Koetter and Frank R. Kschischang %\\cite{DBLP:journals/tit/KoetterK08} introduced...... in the above metric making them suitable for linear network coding....

  6. Small-scale hydro-electric scheme in Roches, Switzerland; Projet de concession. Petite centrale hydroelectrique de Roches

    Energy Technology Data Exchange (ETDEWEB)

    Hausmann, M.

    2006-07-01

    The small hydropower plant (SHPP) project 'PCH Roches' is planned to be built in Roches, a small village located in the Jura region of the canton of Bern. The goal of this undertaking is to reactivate a site with hydro potential of the river Birs on a section that was already well exploited by some ancient SHPP (since 1953 or probably even earlier). Several modifications were performed on this plant over the years; not all contributed positively on its production figures. Following a transfer of the water rights in 1987, claims became loud against the owner to perform maintenance on the river banks and to modify the weir such that a free passage for the fishes will be granted. Those issues contributed to inflate a conflict which ended in the repeal of the water rights and a total plant shut down in 2001. The new project plans to reuse the existing pen stock and to carry out some refurbishing activities to grant security and better performance. The weir and the intake structure are also redesigned such that the water storage can be somewhat raised for normal operation; a weir gate will however allow to better discharge the river in flood situations. A new turbine house will be erected at the current location where the tail water pen stock rejoins the river. Hence, the existing old turbine house will be obsolete and the upper and lower pen stock sections are to be linked with each other. Limited by the existing pen stock size, the new plant is expected to produce some 50 kW power and 362,000 kWh/year. This represents an increase in energy generation of around 200% compared with the ancient SHPP. Gross head is 5.38 m, net head 4.12 m with a turbine flow of at least 1.6 m{sup 3}/s. The expected energy sales price applicable to this project reaches about 0.17 CHF/kWh as per the current tariffs set by the grid owner. This project calls for the building of a state-of-the-art SHPP. It will avoid the total dismantling of the existing works to take place and allow

  7. Tidal Decay and Roche-Lobe Overflow of Gaseous Exoplanets

    Science.gov (United States)

    Jackson, Brian; Jensen, Emily; Peacock, Sarah; Arras, Phil; Penev, Kaloyan

    2016-10-01

    Many gaseous exoplanets in short-period orbits are on the verge or are in the process of Roche-lobe overflow (RLO). Moreover, orbital stability analysis shows tides can drive many hot Jupiters to spiral inevitably toward their host stars, and the distributions of orbital periods and semi-major axes point to non-negligible orbital decay during the main sequence lifetimes of the host stars. Thus, the coupled processes of orbital evolution and RLO likely shape the observed distribution of close-in exoplanets. However, the exact outcome for an overflowing planet depends on its internal response to mass loss, and the accompanying orbital evolution can act to enhance or inhibit RLO. Applying the Modules for Experiments in Stellar Astrophysics (MESA) suite to model RLO, we find that, although the detailed evolution may depend on several properties of the planetary system, it is largely determined by the core mass of the overflowing gas giant. In particular, we find that the orbital expansion that accompanies RLO often stops and reverses at a specific maximum period that depends on the core mass. We suggest that RLO may often strand the remnant of a gas giant near or interior to this orbital period, providing an observational prediction that can corroborate the RLO hypothesis. We conduct a preliminary comparison of this prediction to the observed population of small, short-period planets and find some planets in orbits that may be consistent with this picture.

  8. Disainmööbel Roche Bobois, kokteil sarmist ja romantikast / Reet Krause

    Index Scriptorium Estoniae

    Krause, Reet, 1967-

    2004-01-01

    Luksuslikku kodumööblit tootvast prantsuse firmast. Firma disainerid itaallased Paola Navone ja Massimo Iosa Ghini, Vladimir Kagan, Hans Hopper ja prantslased Jean Claude Magirard ja Sylvain Joly. Philippe Roche ja disainer Hans Hopperi kommentaar

  9. COMPOSITIONS AND ORIGINS OF OUTER PLANET SYSTEMS: INSIGHTS FROM THE ROCHE CRITICAL DENSITY

    Energy Technology Data Exchange (ETDEWEB)

    Tiscareno, Matthew S.; Hedman, Matthew M. [Center for Radiophysics and Space Research, Cornell University, Ithaca, NY 14853 (United States); Burns, Joseph A. [Department of Astronomy, Cornell University, Ithaca, NY 14853 (United States); Castillo-Rogez, Julie [Jet Propulsion Laboratory, Pasadena, CA 91109 (United States)

    2013-03-10

    We consider the Roche critical density ({rho}{sub Roche}), the minimum density of an orbiting object that, at a given distance from its planet, is able to hold itself together by self-gravity. It is directly related to the more familiar ''Roche limit,'' the distance from a planet at which a strengthless orbiting object of given density is pulled apart by tides. The presence of a substantial ring requires that transient clumps have an internal density less than {rho}{sub Roche}. Conversely, in the presence of abundant material for accretion, an orbiting object with density greater than {rho}{sub Roche} will grow. Comparing the {rho}{sub Roche} values at which the Saturn and Uranus systems transition rapidly from disruption-dominated (rings) to accretion-dominated (moons), we infer that the material composing Uranus' rings is likely more rocky, as well as less porous, than that composing Saturn's rings. From the high values of {rho}{sub Roche} at the innermost ring moons of Jupiter and Neptune, we infer that those moons may be composed of denser material than expected, or more likely that they are interlopers that formed farther from their planets and have since migrated inward, now being held together by internal material strength. Finally, the ''Portia group'' of eight closely packed Uranian moons has an overall surface density similar to that of Saturn's A ring. Thus, it can be seen as an accretion-dominated ring system, of similar character to the standard ring systems except that its material has a characteristic density greater than the local {rho}{sub Roche}.

  10. Performance of the Roche LightCycler real-time PCR assay for diagnosing extrapulmonary tuberculosis.

    Science.gov (United States)

    Gous, N; Scott, L E; Wong, E; Omar, T; Venter, W D F; Stevens, W

    2012-06-01

    The Roche LightCycler mycobacterium detection molecular assay for Mycobacterium tuberculosis, M. avium, and M. kansasii, was applied to tissue specimens. It performed well on lymph node and cerebrospinal fluid specimens and less well on lung, liver, and bone marrow core biopsy specimens, but used in conjunction with a clinical suspicion of tuberculosis, it could augment patient management.

  11. The Hirzebruch--Riemann--Roch theorem in true genus-0 quantum K-theory

    CERN Document Server

    Givental, Alexander

    2011-01-01

    We completely characterize genus-0 K-theoretic Gromov-Witten invariants of a compact complex algebraic manifold in terms of cohomological Gromov-Witten invariants of this manifold. This is done by applying (a virtual version of) the Kawasaki-Hirzebruch-Riemann-Roch formula for expressing holomorphic Euler characteristics of orbibundles on moduli spaces of genus-0 stable maps, analyzing the sophisticated combinatorial structure of inertia stacks of such moduli spaces, and employing various quantum Riemann--Roch formulas from "fake" (i.e. orbifold-ignorant) quantum K-theory of manifold and orbifolds (formulas, either previously known from works of Coates-Givental, Tseng, and Coates-Corti-Iritani-Tseng, or newly developed for this purpose in separate papers by Tonita). The ultimate formulation combines properties of overruled Lagrangian cones in symplectic loop spaces (the language, that has become traditional in description of generating functions of genus-0 Gromov-Witten theory) with a novel framework of "adel...

  12. On the Use of Roche Equipotentials in Analysing the Problems of Binary and Rotating Stars

    Indian Academy of Sciences (India)

    A. Pathania; A. K. Lal; C. Mohan

    2013-03-01

    Kopal (Adv. Astron. Astrophys., 9, 1, 1972) introduced the concept of Roche equipotentials to analyse the effects of rotational and tidal distortions in case of stars in binary systems. In this approach a mathematical expression for the potential of a star in a binary system is obtained by approximating its inner structure with Roche model. This expression for the potential has been used in subsequent analysis by various authors to analyse the problems of structures and oscillations of synchronous and nonsynchronus binary stars as well as single rotating stars. Occasionally, doubts have been expressed regarding the validity of the use of this approach for analysing nonsynchronous binaries and rotationally and tidally distorted single stars. In this paper we have tried to clarify these doubts.

  13. Tidally-driven Roche-Lobe Overflow of Hot Jupiters with MESA

    CERN Document Server

    Valsecchi, Francesca; Rasio, Frederic A; Marchant, Pablo; Rogers, Leslie A

    2015-01-01

    Many exoplanets have now been detected in orbits with ultra-short periods, very close to the Roche limit. Building upon our previous work, we study the possibility that mass loss through Roche lobe overflow (RLO) may affect the evolution of these planets, and could possibly transform a hot Jupiter into a lower-mass planet (hot Neptune or super-Earth). We focus here on systems in which the mass loss occurs slowly ("stable mass transfer" in the language of binary star evolution) and we compute their evolution in detail with the binary evolution code MESA. We include the effects of tides, RLO, irradiation and photo-evaporation of the planet, as well as the stellar wind and magnetic braking. Our calculations all start with a hot Jupiter close to its Roche limit, in orbit around a sun-like star. The initial orbital decay and onset of RLO are driven by tidal dissipation in the star. We confirm that such a system can indeed evolve to produce lower-mass planets in orbits of a few days. The RLO phase eventually ends a...

  14. Galaxies with Supermassive Binary Black Holes: (III) The Roche Lobes and Jiang-Yeh Lobe in a Core System

    CERN Document Server

    Yeh, Li-Chin

    2016-01-01

    Three-dimensional equi-potential surfaces of a galactic system with supermassive binary black holes are discussed herein. The conditions of topological transitions for the important surfaces, i.e. Roche Lobes and Jiang-Yeh Lobe, are studied in this paper. In addition, the mathematical properties of the Jacobi surfaces are investigated analytically. Finally, a numerical procedure for determining the regions of the Roche Lobes and Jiang-Yeh Lobe is suggested.

  15. Exo-Mercury Analogues and the Roche Limit for Close-Orbiting Rocky Planets

    Science.gov (United States)

    Rogers, Leslie A.; Price, Ellen

    2015-12-01

    The origin of Mercury's enhanced iron content is a matter of ongoing debate. The characterization of rocky exoplanets promises to provide new independent insights on this topic, by constraining the occurrence rate and physical and orbital properties of iron-enhanced planets orbiting distant stars. The ultra-short-period transiting planet candidate KOI-1843.03 (0.6 Earth-radius, 4.245 hour orbital period, 0.46 Solar-mass host star) represents the first exo-Mercury planet candidate ever identified. For KOI-1843.03 to have avoided tidal disruption on such a short orbit, Rappaport et al. (2013) estimate that it must have a mean density of at least 7g/cc and be at least as iron rich as Mercury. This density lower-limit, however, relies upon interpolating the Roche limits of single-component polytrope models, which do not accurately capture the density profiles of >1000 km differentiated rocky bodies. A more exact calculation of the Roche limit for the case of rocky planets of arbitrary composition and central concentration is needed. We present 3D interior structure simulations of ultra-short-period tidally distorted rocky exoplanets, calculated using a modified version of Hachisu’s self-consistent field method and realistic equations of state for silicates and iron. We derive the Roche limits of rocky planets as a function of mass and composition, and refine the composition constraints on KOI-1843.03. We conclude by discussing the implications of our simulations for the eventual characterization of short-period transiting planets discovered by K2, TESS, CHEOPS and PLATO.

  16. Tidally-driven Roche-lobe Overflow of Hot Jupiters with MESA

    Science.gov (United States)

    Valsecchi, Francesca; Rappaport, Saul; Rasio, Frederic A.; Marchant, Pablo; Rogers, Leslie A.

    2015-11-01

    Many exoplanets have now been detected in orbits with ultra-short periods very close to the Roche limit. Building upon our previous work, we study the possibility that mass loss through Roche lobe overflow (RLO) may affect the evolution of these planets, and could possibly transform a hot Jupiter into a lower-mass planet (hot Neptune or super-Earth). We focus here on systems in which the mass loss occurs slowly (“stable mass transfer” in the language of binary star evolution) and we compute their evolution in detail with the binary evolution code Modules for Experiments in Stellar Astrophysics. We include the effects of tides, RLO, irradiation, and photo-evaporation (PE) of the planet, as well as the stellar wind and magnetic braking. Our calculations all start with a hot Jupiter close to its Roche limit, in orbit around a Sun-like star. The initial orbital decay and onset of RLO are driven by tidal dissipation in the star. We confirm that such a system can indeed evolve to produce lower-mass planets in orbits of a few days. The RLO phase eventually ends and, depending on the details of the mass transfer and on the planetary core mass, the orbital period can remain around a few days for several Gyr. The remnant planets have rocky cores and some amount of envelope material, which is slowly removed via PE at a nearly constant orbital period; these have properties resembling many of the observed super-Earths and sub-Neptunes. For these remnant planets, we also predict an anti-correlation between mass and orbital period; very low-mass planets (Mpl ≲ 5 M⊕) in ultra-short periods (Porb < 1 day) cannot be produced through this type of evolution.

  17. Wind Roche-lobe overflow: Application to carbon-enhanced metal-poor stars

    CERN Document Server

    Abate, C; Izzard, R G; Mohamed, S S; de Mink, S E

    2013-01-01

    Carbon-enhanced metal-poor stars (CEMP) are observed as a substantial fraction of the very metal-poor stars in the Galactic halo. Most CEMP stars are also enriched in s-process elements and these are often found in binary systems. This suggests that the carbon enrichment is due to mass transfer in the past from an asymptotic giant branch (AGB) star on to a low-mass companion. Models of binary population synthesis are not able to reproduce the observed fraction of CEMP stars without invoking non-standard nucleosynthesis or a substantial change in the initial mass function. This is interpreted as evidence of missing physical ingredients in the models. Recent hydrodynamical simulations show that efficient wind mass transfer is possible in the case of the slow and dense winds typical of AGB stars through a mechanism called wind Roche-lobe overflow (WRLOF), which lies in between the canonical Bondi-Hoyle-Lyttleton (BHL) accretion and Roche-lobe overflow. WRLOF has an effect on the accretion efficiency of mass tran...

  18. Roche tomography of cataclysmic variables - V. A high-latitude star-spot on RU Pegasi

    CERN Document Server

    Dunford, Alexander; Smith, Robert Connon

    2012-01-01

    We present Roche tomograms of the secondary star in the dwarf nova system RU Pegasi derived from blue and red arm ISIS data taken on the 4.2-m William Herschel Telescope. We have applied the entropy landscape technique to determine the system parameters and obtained component masses of M1 = 1.06 Msun, M2 = 0.96 Msun, an orbital inclination angle of i = 43 degrees, and an optimal systemic velocity of gamma = 7 km/s. These are in good agreement with previously published values. Our Roche tomograms of the secondary star show prominent irradiation of the inner Lagrangian point due to illumination by the disc and/or bright spot, which may have been enhanced as RU Peg was in outburst at the time of our observations.We find that this irradiation pattern is axi-symmetric and confined to regions of the star which have a direct view of the accretion regions. This is in contrast to previous attempts to map RU Peg which suggested that the irradiation pattern was non-symmetric and extended beyond the terminator. We also d...

  19. Roche-lobe filling factor of mass-transferring red giants - the PIONIER view

    CERN Document Server

    Boffin, Henri M J; Berger, J P; Jorissen, A; Blind, N; Bouquin, J B Le; Mikolajewska, J; Lazareff, B

    2014-01-01

    Using the PIONIER visitor instrument that combines the light of the four Auxiliary Telescopes of ESO's Very Large Telescope Interferometer, we measure precisely the diameters of several symbiotic and related stars: HD 352, HD 190658, V1261 Ori, ER Del, FG Ser, and AG Peg. These diameters - in the range of 0.6 to 2.3 milli-arcseconds - are used to assess the filling factor of the Roche lobe of the mass-losing giants and provide indications on the nature of the ongoing mass transfer. We also provide the first spectroscopic orbit of ER Del, based on CORAVEL and HERMES/Mercator observations. The system is found to have an eccentric orbit with a period of 5.7 years. In the case of the symbiotic star FG Ser, we find that the diameter is changing by 13% over the course of 41 days, while the observations of HD 352 are indicative of an elongation. Both these stars are found to have a Roche filling factor close to 1, as is most likely the case for HD 190658 as well, while the three other stars have factors below 0.5-0....

  20. Robe's restricted problem of 2+2 bodies when the bigger primary is a Roche ellipsoid

    Science.gov (United States)

    Kaur, Bhavneet; Aggarwal, Rajiv

    2013-08-01

    In the problem of 2 + 2 bodies in Robe's setup, one of the primaries of mass m1 is a Roche ellipsoid filled with a homogeneous incompressible fluid of density ρ1. The smaller primary of mass m2 is a point mass outside the ellipsoid. The third and the fourth bodies (of mass m3 and m4, respectively), supposed moving inside the ellipsoid, are small solid spheres of density ρ3 and ρ4, respectively, with the assumption that the mass and the radius of the third and the fourth bodies are infinitesimal. We assume that m2 is describing a circle around m1. The masses m3 and m4 mutually attract each other, do not influence the motions of m1 and m2 but are influenced by them. Robe's restricted three body problem is extended to 2 + 2 body problem under the assumption that the fluid body assumes the shape of the Roche ellipsoid (Chandrashekhar [2]). We take into consideration all the three components of the pressure field in deriving the expression for the buoyancy force viz (i) due to the own gravitational field of the fluid, (ii) that originating in the attraction of m2, and (iii) that arising from the centrifugal force. In this paper, equilibrium solutions of m3 and m4 and their linear stability are analysed. We have proved that there exist only six equilibrium solutions of the system. In a system where the primaries are considered as earth-moon and m3 ,m4 as submarines, the equilibrium solutions thus obtained are unstable.

  1. An in-house real-time polymerase chain reaction: standardisation and comparison with the Cobas Amplicor HBV monitor and Cobas AmpliPrep/Cobas TaqMan HBV tests for the quantification of hepatitis B virus DNA

    Science.gov (United States)

    Santos, Ana Paula de Torres; Levi, José Eduardo; Lemos, Marcilio Figueiredo; Calux, Samira Julien; Oba, Isabel Takano; Moreira, Regina Célia

    2016-01-01

    This study aimed to standardise an in-house real-time polymerase chain reaction (rtPCR) to allow quantification of hepatitis B virus (HBV) DNA in serum or plasma samples, and to compare this method with two commercial assays, the Cobas Amplicor HBV monitor and the Cobas AmpliPrep/Cobas TaqMan HBV test. Samples from 397 patients from the state of São Paulo were analysed by all three methods. Fifty-two samples were from patients who were human immunodeficiency virus and hepatitis C virus positive, but HBV negative. Genotypes were characterised, and the viral load was measure in each sample. The in-house rtPCR showed an excellent success rate compared with commercial tests; inter-assay and intra-assay coefficients correlated with commercial tests (r = 0.96 and r = 0.913, p < 0.001) and the in-house test showed no genotype-dependent differences in detection and quantification rates. The in-house assay tested in this study could be used for screening and quantifying HBV DNA in order to monitor patients during therapy. PMID:26872342

  2. Roche tomography of cataclysmic variables - VII. The long-term magnetic activity of AE Aqr

    Science.gov (United States)

    Hill, C. A.; Watson, C. A.; Steeghs, D.; Dhillon, V. S.; Shahbaz, T.

    2016-06-01

    We present a long-term study of the secondary star in the cataclysmic variable AE Aqr, using Roche tomography to indirectly image starspots on the stellar surface spanning 8 years of observations. The seven maps show an abundance of spot features at both high and low latitudes. We find that all maps have at least one large high-latitude spot region, and we discuss its complex evolution between maps, as well as its compatibility with current dynamo theories. Furthermore, we see the apparent growth in fractional spot coverage, fs, around 45° latitude over the duration of observations, with a persistently high fs near latitudes of 20°. These bands of spots may form as part of a magnetic activity cycle, with magnetic flux tubes emerging at different latitudes, similar to the `butterfly' diagram for the Sun. We discuss the nature of flux tube emergence in close binaries, as well as the activity of AE Aqr in the context of other stars.

  3. Structures in the D ring and Roche division tied to asymmetries in Saturn's magnetosphere

    Science.gov (United States)

    Chancia, Robert O.; Hedman, Matthew M.; Carter, Brian

    2016-10-01

    Saturn's dusty rings contain multiple patterns that appear to be rotating around the planet at about the same rate as Saturn itself, and so are probably generated by resonances with asymmetries in the planet's gravitational or magnetic fields. These structures are found in the D ring (which lies interior to the main rings) and in the Roche Division (which is located just outside the main rings, between the A and F rings). In 2007 the strongest patterns in both of these regions appeared to track magnetospheric anomalies associated with the Saturn Kilometric Radiation (SKR). This implied that these rings were responding to structures in the planet's magnetosphere, which is not unreasonable since the particles in both these ring regions are very small and therefore sensitive to non-gravitational forces. Over the last few years, the frequencies of the SKR and related magnetospheric asymmetries have shifted, and we have observed some changes in the ring patterns that might be connected with these shifts. However, there are also patterns in these rings that appear to have more stable rotation rates and so could reflect more persistent asymmetries in Saturn's magnetosphere. These patterns can therefore provide novel insights into the structure and evolution of Saturn's magnetosphere.

  4. The N(o)ther and Riemann-Roch type theorems for piecewise algebraic curve

    Institute of Scientific and Technical Information of China (English)

    Yi-sheng LAI; Ren-hong WANG

    2007-01-01

    A piecewise algebraic curve is a curve determined by the zero set of a bivariate spline function. In this paper, the N(o)ther type theorems for Cμ piecewise algebraic curves are obtained.The theory of the linear series of sets of places on the piecewise algebraic curve is also established.In this theory, singular cycles are put into the linear series, and a complete series of the piecewise algebraic curves consists of all effective ordinary cycles in an equivalence class and all effective singular cycles which are equivalent specifically to any effective ordinary cycle in the equivalence class. This theory is a generalization of that of linear series of the algebraic curve. With this theory and the fundamental theory of multivariate splines on smoothing cofactors and global conformality conditions,and the results on the general expression of multivariate splines, we get a formula on the index, the order and the dimension of a complete series of the irreducible Cμ piecewise algebraic curves and the degree, the genus and the smoothness of the curves, hence the Riemann-Roch type theorem of the Cμpiecewise algebraic curve is established.

  5. Roche lobe underfilling of the secondary star in PSR J102347.6+003841?

    Science.gov (United States)

    McConnell, O.; Callanan, P. J.; Kennedy, M.; Hurley, D.; Garnavich, P.; Menzies, J.

    2015-08-01

    PSR J102347.6+003841 is a radio pulsar system with a spin period of 1.69 ms and an orbital period of 4.75 h. Uniquely, it undergoes periods of transient accretion from its companion star: it occupies an important position in the evolutionary track from X-ray binary to isolated millisecond radio pulsar. Here, we present a spectroscopic study of this system showing late-type absorption features which match those of a G2V star. We find a semi-amplitude of 286 ± 3 kms-1 and a best-fitting orbital period of 0.198 0966(1) d. We combine these measurements with optical photometry which suggests the secondary star may be underfilling its Roche lobe by between 15 and 20 per cent. We weakly constrain the mass of the neutron star to be ≤2.2 M⊙ at the 2σ level. We also discuss the possible origins of the Hα emission line in our template subtracted, averaged spectrum. Finally, we present and discuss new optical photometry of J1023 taken during the recent outburst of the system.

  6. Roche Lobe Underfilling of the Secondary Star in PSR J102347.6+003841?

    CERN Document Server

    McConnell, O; Kennedy, M; Hurley, D; Garnavich, P; Menzies, J

    2015-01-01

    PSR J102347.6+003841 is a radio pulsar system with a spin period of 1.69 ms and an orbital period of 4.75 hours. Uniquely, it undergoes periods of transient accretion from its companion star: it occupies an important position in the evolutionary track from X-ray binary to isolated millisecond radio pulsar. Here we present a spectroscopic study of this system showing late-type absorption features which match those of a G2V star. We find a semiamplitude of $286 \\pm 3$ kms$^{-1}$ and a best fit orbital period of 0.1980966(1) days. We combine these measurements with optical photometry which suggests the secondary star may be underfilling its Roche lobe by between 15\\% and 20\\%. We weakly constrain the mass of the neutron star to be $\\leq$ 2.2 M$_\\odot$ at the 2$\\sigma$ level. We also discuss the possible origins of the H$\\alpha$ emission line in our template subtracted, averaged spectrum. Finally we present and discuss new optical photometry of J1023 taken during the recent outburst of the system.

  7. The evolution of triples with a Roche-lobe filling outer star

    CERN Document Server

    de Vries, Nathan; Figueira, Joana

    2013-01-01

    The evolution of triples has not attracted much attention in the literature, although their evolution can be dramatically different from binaries and single stars. Triples are quite common, and we find that for about 1% of the triples in the Tokovinin catalogue of multiple stellar systems in the solar neighbourhood, the tertiary star will overflow its Roche lobe at some time in its evolution, before any of the inner stars leave the main sequence. For two of these systems, Xi Tauri and HD97131 we simulate in detail this phase of mass transfer, during which stellar evolution, gravitational dynamics and hydrodynamics all play an important role. We have used the Astrophysical Multi-purpose Software Environment (AMUSE) to solve these physical processes in a self-consistent way. The resulting evolution, mass transfer and the effects on the inner as well as on the outer orbit are profound, although it is not trivial to predict the eventual consequence of the phase of mass transfer and the appearance of the resulting...

  8. Evaluation of the Roche CoaguChek XS handheld coagulation analyzer in a cardiac outpatient clinic.

    Science.gov (United States)

    Nam, Myung-Hyun; Roh, Kyoung Ho; Pak, Hui-Nam; Lee, Chang Kyu; Kim, Young-Hoon; Lee, Kap No; Cho, Yunjung

    2008-01-01

    This study evaluated the performance of a handheld coagulation analyzer for measurements of capillary blood specimens of 93 outpatient cardiology patients with atrial fibrillation who were receiving oral anti-coagulant therapy. The international normalized ratio (INR) results of the CoaguChek XS system (Roche Diagnostics) were compared with those obtained in the central laboratory with citrated venous blood specimens using the ACL9000 coagulation analyzer (Instrumentation Laboratory). The INR results for prothrombin time by the CoaguChek XS analyzer were closely correlated with the central laboratory's results in the INR range of 0.96 approximately 8.53 (r = 0.964). A statistically significant difference was noted between 2 lots of test strips, but the difference was miniscule (mean +/- 95% confidence interval: 0.04+/-0.02). The CV of 8 replicate assays with the CoaguChek XS for a blood specimen with high INR value (INR=3.9) was 1.4%; for a blood specimen with medium INR value (INR=1.3), the CV of 8 replicate assays was XS analyzer is precise and reliable for assessment of INR results at clinically significant ranges in cardiac outpatients.

  9. Roche tomography of cataclysmic variables - VII. The long-term magnetic activity of AE Aqr

    CERN Document Server

    Hill, C A; Steeghs, D; Dhillon, V S; Shahbaz, T

    2016-01-01

    We present a long-term study of the secondary star in the cataclysmic variable AE~Aqr, using Roche tomography to indirectly image starspots on the stellar surface spanning 8~years of observations. The 7 maps show an abundance of spot features at both high and low latitudes. We find that all maps have at least one large high-latitude spot region, and we discuss its complex evolution between maps, as well as its compatibility with current dynamo theories. Furthermore, we see the apparent growth in fractional spot coverage, $f_{\\mathrm{s}}$, around $45^{\\circ}$~latitude over the duration of observations, with a persistently high $f_{\\mathrm{s}}$ near latitudes of $20^{\\circ}$. These bands of spots may form as part of a magnetic activity cycle, with magnetic flux tubes emerging at different latitudes, similar to the `butterfly' diagram for the Sun. We discuss the nature of flux tube emergence in close binaries, as well as the activity of AE~Aqr in the context of other stars.

  10. Polyphony and counterpoint: Mechanisms of seduction in the diaries of Helen Hessel and Henri Pierre Roché

    Directory of Open Access Journals (Sweden)

    Catherine du Toit

    2015-06-01

    Full Text Available Henri Pierre Roché (1879–1959, the author of Jules et Jim, has been called a general introducer, an exemplary amateur, a collector of women and art and one of the most prolific diarists and active lovers in recorded history. Author of a collection of vignettes about Don Juan, Roché was fascinated with the figure of the seducer and in his twenties planned to devote his life to the creation of a body of work which would examine moral, intellectual, social and sexual relations between women and men. To this end, he would transform his life into a laboratory where real-life experiences would become the main source of reference. Roché’s diary spans sixty years and abounds in tales of seduction. However, the most intense and captivating intrigue of seduction and betrayal in his diary, is his relationship with Helen Hessel. At the start of their affair, Roché suggested that she too should keep a diary of the maelstrom of passion into which they were plunged. Written in French, German and English, Helen Hessel’s diary captures the drama of seduction and functions on several levels: realistic, visionary, absorbed in her thoughts and emotions and yet critical of herself and others. A juxtaposed reading of the two diaries generates a fascinatingly dense texture, revealing the mechanisms of seduction at play. The counterpoint created by these two interdependent voices becomes ever more complex as one becomes aware of the intertextual references that contribute to the emerging polyphony of recorded life and love.Polifonie en kontrapunt: Meganismes van verleiding in die dagboeke van Helen Hessel en Henri Pierre Roché. Henri Pierre Roché (1879–1959, outeur van Jules et Jim, word beskryf as ‘n sosiale koppelaar, ‘n model-liefhebber van alles en nog wat, ‘n versamelaar van vroue en kuns en een van die mees produktiewe dagboekskrywers en aktiewe minnaars in die opgetekende geskiedenis. Roché het ‘n reeks sketse oor Don Juan gepubliseer en

  11. Tidal decay and stable Roche-lobe overflow of short-period gaseous exoplanets

    Science.gov (United States)

    Jackson, Brian; Jensen, Emily; Peacock, Sarah; Arras, Phil; Penev, Kaloyan

    2016-06-01

    Many gaseous exoplanets in short-period orbits are on the verge or are in the process of Roche-lobe overflow (RLO). Moreover, orbital stability analysis shows tides can drive many hot Jupiters to spiral inevitably toward their host stars. Thus, the coupled processes of orbital evolution and RLO likely shape the observed distribution of close-in exoplanets and may even be responsible for producing some of the short-period rocky planets. However, the exact outcome for an overflowing planet depends on its internal response to mass loss, and the accompanying orbital evolution can act to enhance or inhibit RLO. In this study, we apply the fully-featured and robust Modules for Experiments in Stellar Astrophysics suite to model RLO of short-period gaseous planets. We show that, although the detailed evolution may depend on several properties of the planetary system, it is largely determined by the core mass of the overflowing gas giant. In particular, we find that the orbital expansion that accompanies RLO often stops and reverses at a specific maximum period that depends on the core mass. We suggest that RLO may often strand the remnant of a gas giant near this orbital period, which provides an observational prediction that can corroborate the hypothesis that short-period gas giants undergo RLO. We conduct a preliminary comparison of this prediction to the observed population of small, short-period planets and find some planets in orbits that may be consistent with this picture. To the extent that we can establish some short-period planets are indeed the remnants of gas giants, that population can elucidate the properties of gas giant cores, the properties of which remain largely unconstrained.

  12. Some physiological changes in Waldsteinia trifolia (Roch. leaves in different months of the year

    Directory of Open Access Journals (Sweden)

    Renata Wojciechowska

    2012-12-01

    Full Text Available The study concerned an evergreen herbal plant Waldsteinia trifolia Roch. This species can be found more and more often in garden plantings in Poland. The aim of the study was to estimate the content of photosynthetic pigments, anthocyanins and dry matter as well as the permeability of cytoplasmatic membranes of Waldsteinia leaves. Plants grew in an ornamental plant collection at the premises of the Faculty of Horticulture of the Agricultural University in Kraków. The study was conducted in 2006 and 2007 in the following months: March, May, June, September, October and November. The permeability of cytoplasmatic membranes was estimated based on electrolyte leakage from leaf discs at 20°C in relation to total electrolytes in the tissue after unfreezing. The first days with temperature drops down to around 0°C in autumn resulted in a decrease in chlorophyll a, b and carotenoid content, but significantly increased anthocyanin content. The ratio of chlorophyll a to b and the ratio of total chlorophylls to carotenoids did not change in particular months. In both years of study, similar changes in dry matter content of the Waldsteinia leaves were observed. Dry matter content significantly increased in June and November and decreased in May and October. In June and in autumn, after first slight frosts, an increase of electrolyte leakage from the leaf discs was observed. However, high dry matter content (40.3% was accompanied by lower permeability of the cytoplasmatic membranes of the leaves after winter during March. These results have shown good adaptation of Waldsteinia trifolia plants to the climatic conditions in Poland.

  13. High-throughput sequencing of core STR loci for forensic genetic investigations using the Roche Genome Sequencer FLX platform

    DEFF Research Database (Denmark)

    Fordyce, Sarah L; Avila-Arcos, Maria C; Rockenbauer, Eszter;

    2011-01-01

    The analysis and profiling of short tandem repeat (STR) loci is routinely used in forensic genetics. Current methods to investigate STR loci, including PCR-based standard fragment analyses and capillary electrophoresis, only provide amplicon lengths that are used to estimate the number of STR...... repeat units. These methods do not allow for the full resolution of STR base composition that sequencing approaches could provide. Here we present an STR profiling method based on the use of the Roche Genome Sequencer (GS) FLX to simultaneously sequence multiple core STR loci. Using this method...

  14. Whole-Exome Enrichment with the Roche NimbleGen SeqCap EZ Exome Library SR Platform

    OpenAIRE

    Chen, Rui; Im, Hogune; Snyder, Michael

    2015-01-01

    Multiple platforms are available for whole-exome enrichment and sequencing (WES). This protocol is based on the Roche NimbleGen SeqCap EZ Exome Library SR platform, which enriches for ~44 Mb of the human exonic regions. The SeqCap system uses 55- to 105-base DNA probes to capture known coding DNA sequences (CDS) from the NCBI Consensus CDS Database, RefSeq, and Sanger miRBase. The protocol can be performed at the benchside without the need for automation, and the resulting library can be used...

  15. 基于 Roche 454 GS FLX 的钝吻黄盖鲽(Pleuronectes yokohamae)微卫星标记的开发%Microsatellite Loci Isolated by Roche 454 GS FLX in Pleuronectes yokohamae

    Institute of Scientific and Technical Information of China (English)

    潘婷; 张岩; 张辉; 高天翔; 肖永双; 姜云荣

    2015-01-01

    Roche 454 GS FLX, the second-generation sequencing technique, can read longer fragments accurately and isolate SSR markers, and thus has been widely used in many fields of research. In this study, we applied Roche 454 GS FLX to isolate the microsatellite loci and developed the identification of microsatellites markers in Pleuronectes yokohamae. (AC)12, (AG)12, (AAT)12, (AGG)8, (AGC)8, (AGAT)8 and (ACAG)6 were hybridized with shotgun library of DNA samples. This SSR library was constructed through magnetic beads enrichment, cleaning, elution and purification. The SSR library was then sequenced with Roche 454 GS FLX. MISA was used to search for microsatellite motifs and primer 3 was used to design the primers for P. yokohamae. FAM, HEX, and TAMRA were used to label the microsatellite primers (5′), which were used in the triplex PCR or the nested PCR to identify the genotype of the microsatellite loci. The cluster similarity comparison method was first used to analyze 5641 loci that we obtained, and 247 types of loci with high polymorphism were screened. Among these loci, 52.22% were perfect, 20.24% were imperfect and the rest 27.54% were compound. The percentage of (AC)n and (AG)n was 44% in these loci, and 87.5% of them were more than 10-time repeats. Eleven pairs of fluorescent primers were designed according to the selected loci (more than 10-time repeats) and were applied in 5 individuals. The number of alleles per locus ranged from 3 to 8 with an average value of 5. The average values of Ho, He and PIC were 0.588, 0.788 and 0.670 respectively. Four loci among 11 deviated from Hardy-Weinberg. The results of this research indicated that 454 GS FLX was an intuitive and efficient technique for microsatellite isolating. Our study provides essential information about the population genetic diversity of P. yokohamae, and helps improve the artificial breeding.%利用 Roche 454 GS FLX 平台测序技术进行了钝吻黄盖鲽微卫星引物筛选,并采用聚

  16. Microsatellite Loci Isolated by Roche 454 GS FLX in Pleuronectes yokohamae%基于 Roche 454 GS FLX 的钝吻黄盖鲽(Pleuronectes yokohamae)微卫星标记的开发

    Institute of Scientific and Technical Information of China (English)

    潘婷; 张岩; 张辉; 高天翔; 肖永双; 姜云荣

    2015-01-01

    利用 Roche 454 GS FLX 平台测序技术进行了钝吻黄盖鲽微卫星引物筛选,并采用聚类分析的方法对得到的5641个微卫星位点进行类比分析,得到247种多态性位点,其中完美型占52.22%,非完美型占20.24%,复合型占27.54%,(AC)n、(AG)n 两碱基重复类型的比例是44%,重复次数在10次以上的占总数的87.5%。随机选取11个位点的微卫星引物,采用5个野生个体,利用荧光标记和毛细管电泳进行多样性评价,4个位点偏离 Hardy-Weinberg 平衡,不同位点得到的等位基因范围为3−8,平均等位基因数为5.0。平均观望杂合度(Ho)、平均期望杂合度(He)及平均多态性信息含量(PIC)分别为0.588、0.788和0.670。结果表明,454 GS FLX 提供了一种直观、高效开发微卫星的方法。%Roche 454 GS FLX, the second-generation sequencing technique, can read longer fragments accurately and isolate SSR markers, and thus has been widely used in many fields of research. In this study, we applied Roche 454 GS FLX to isolate the microsatellite loci and developed the identification of microsatellites markers in Pleuronectes yokohamae. (AC)12, (AG)12, (AAT)12, (AGG)8, (AGC)8, (AGAT)8 and (ACAG)6 were hybridized with shotgun library of DNA samples. This SSR library was constructed through magnetic beads enrichment, cleaning, elution and purification. The SSR library was then sequenced with Roche 454 GS FLX. MISA was used to search for microsatellite motifs and primer 3 was used to design the primers for P. yokohamae. FAM, HEX, and TAMRA were used to label the microsatellite primers (5′), which were used in the triplex PCR or the nested PCR to identify the genotype of the microsatellite loci. The cluster similarity comparison method was first used to analyze 5641 loci that we obtained, and 247 types of loci with high polymorphism were screened. Among these loci, 52.22% were perfect, 20.24% were imperfect and the rest 27.54% were compound

  17. Paleotectonic Settings for Petroleum Source Rocks Position paléolithique des roches mères

    Directory of Open Access Journals (Sweden)

    Curtis D. M.

    2006-10-01

    Full Text Available Petroleum source rocks have been deposited in a variety of paleotectonic and paleogeographic settings. The paleotectonic setting for a given source rock is, in many cases, different from the present tectonic setting of the basin in which the derived hydrocarbon accumulations are found. The quantity and type of hydrocarbons generated from a given source rock depend upon the quantity (richness, quality (kerogen type, and maturity (ability to generate hydrocarbons of the organic carbon in it. These three factors are strongly influenced by the paleotectonic setting of the basin in which a given source rock was deposited, as well as by its subsequent burial history (time-temperature history. The evolution of traps, on the other hand, depends upon the tectonic history of the basin during migration and entropment, and is closely related to the present tectonic setting ot the basin. Using a basin classification based on that of A. W. Bally and modified by K. Arbenz, I have attempted to related the distribution of hydrocarbon reserves in some of the major well-explored basins of the world to the paleotectonic settings for known or postulated source rocks in those basins. The principal basin categories defined are: cratonic, passive margin-related, aulacogenic. A-subduction-related, B-subduction-related, and strike-slip, transform-related. Preliminary results suggest that some of the best source rocks were deposited in aulacogenic and strike-slip/transform-related settings; some of the poorest were deposited in cratonic settings. Les roches mères de pétrole se sont déposées dans des sites paléotectoniques et paléogéographiques variés. La position paléotectonique d'une roche mère est dans de nombreux cas différente de la situation tectonique actuelle du bassin où l'on trouve les hydrocarbures qui en proviennent. La quantité et le type des hydrocarbures produits par une roche mère donnée dépendent de l'abondance (richesse, de la qualit

  18. Clinical performance of the new Roche COBAS (R) TaqMan HCV test and high pure system for extraction, detection and quantitation of HCV RNA in plasma and serum

    NARCIS (Netherlands)

    H.C. Gelderblom; S. Menting; M.G. Beld

    2006-01-01

    We evaluated the Roche COBAS (R) TaqMan HCV Test For Use With The High Pure System (TaqMan HPS; Roche Diagnostics), for the extraction, detection and quantitation of hepatitis C virus (HCV) RNA in serum or plasma of HCV-infected individuals. The TaqMan HPS is a real-time PCR assay with a reported li

  19. Testing single-grain quartz OSL methods using sediment samples with independent age control from the Bordes-Fitte rockshelter (Roches d'Abilly site, Central France)

    DEFF Research Database (Denmark)

    Thomsen, Kristina Jørkov; Murray, Andrew Sean; Buylaert, Jan-Pieter;

    2016-01-01

    We present quartz single-grain dose distributions for four well-bleached and unmixed sediment samples with independent age control (22–48 ka), from the archaeologically important Bordes-Fitte rockshelter at Roches d'Abilly, France. This site has previously been dated using 14C AMS dating and stan...

  20. Comparison of Roche MONITOR and Organon Teknika NucliSens assays to quantify human immunodeficiency virus type 1 RNA in cerebrospinal fluid.

    Science.gov (United States)

    Spearman, P; Fiscus, S A; Smith, R M; Shepard, R; Johnson, B; Nicotera, J; Harris, V L; Clough, L A; McKinsey, J; Haas, D W

    2001-04-01

    We compared Roche MONITOR and Organon Teknika NucliSens assays for human immunodeficiency virus type 1 (HIV-1) RNA in cerebrospinal fluid (CSF). Results of 282 assays were highly correlated (r = 0.826), with MONITOR values being 0.29 +/- 0.4 log(10) copies/ml (mean +/- standard deviation) values. Both assays can reliably quantify HIV-1 RNA in CSF.

  1. Diseño de estrategias para optimizar la calidad de los servicios de la Biblioteca Marcel Roche del Instituto Venezolano de Investigaciones Científicas (IVIC)

    OpenAIRE

    Echezuria Perozo, Jorge Enrique; Rondon, Maria Carolina; Gutierrez Luna, Melany

    2006-01-01

    As to design new strategies for the quality improvement of all services offered by the Marcel Roche Library (MRL) of the Venezuelan Institute of Scientific Investigations (IVIC) this work studies the beginnings and evolution of quality management improvement reviewing some of the studies presented by some of the most outstanding authors in this subject as well as analyzing the basic components and principles of the total quality management (TQM). This document also focuses on the analysis of ...

  2. From Roche Vitamins to DSM Nutritional Products%从罗氏维生素到帝斯曼营养产品

    Institute of Scientific and Technical Information of China (English)

    2004-01-01

    In October 2003, the Dutch group DSM has acquired the Vitamins and Fine Chemicals business from Roche to create DSM Nutritional Products-one of the world's leading suppliers of vitamins and carotenoids for Animal Nutrition industry, the Food and Pharmaceutical industries as well as the Cosmetics industry by offering a wide range of products to help improve nutrition and prevent disease. In China market, B vitamins are provided by DSM Nutritional Products for food fortification.

  3. Analysis of 16S rRNA amplicon sequencing options on the Roche/454 next-generation titanium sequencing platform.

    Directory of Open Access Journals (Sweden)

    Hideyuki Tamaki

    Full Text Available BACKGROUND: 16S rRNA gene pyrosequencing approach has revolutionized studies in microbial ecology. While primer selection and short read length can affect the resulting microbial community profile, little is known about the influence of pyrosequencing methods on the sequencing throughput and the outcome of microbial community analyses. The aim of this study is to compare differences in output, ease, and cost among three different amplicon pyrosequencing methods for the Roche/454 Titanium platform METHODOLOGY/PRINCIPAL FINDINGS: The following three pyrosequencing methods for 16S rRNA genes were selected in this study: Method-1 (standard method is the recommended method for bi-directional sequencing using the LIB-A kit; Method-2 is a new option designed in this study for unidirectional sequencing with the LIB-A kit; and Method-3 uses the LIB-L kit for unidirectional sequencing. In our comparison among these three methods using 10 different environmental samples, Method-2 and Method-3 produced 1.5-1.6 times more useable reads than the standard method (Method-1, after quality-based trimming, and did not compromise the outcome of microbial community analyses. Specifically, Method-3 is the most cost-effective unidirectional amplicon sequencing method as it provided the most reads and required the least effort in consumables management. CONCLUSIONS: Our findings clearly demonstrated that alternative pyrosequencing methods for 16S rRNA genes could drastically affect sequencing output (e.g. number of reads before and after trimming but have little effect on the outcomes of microbial community analysis. This finding is important for both researchers and sequencing facilities utilizing 16S rRNA gene pyrosequencing for microbial ecological studies.

  4. Characterization of rainbow trout gonad, brain and gill deep cDNA repertoires using a Roche 454-Titanium sequencing approach.

    Science.gov (United States)

    Le Cam, Aurélie; Bobe, Julien; Bouchez, Olivier; Cabau, Cédric; Kah, Olivier; Klopp, Christophe; Lareyre, Jean-Jacques; Le Guen, Isabelle; Lluch, Jérôme; Montfort, Jérôme; Moreews, Francois; Nicol, Barbara; Prunet, Patrick; Rescan, Pierre-Yves; Servili, Arianna; Guiguen, Yann

    2012-05-25

    Rainbow trout, Oncorhynchus mykiss, is an important aquaculture species worldwide and, in addition to being of commercial interest, it is also a research model organism of considerable scientific importance. Because of the lack of a whole genome sequence in that species, transcriptomic analyses of this species have often been hindered. Using next-generation sequencing (NGS) technologies, we sought to fill these informational gaps. Here, using Roche 454-Titanium technology, we provide new tissue-specific cDNA repertoires from several rainbow trout tissues. Non-normalized cDNA libraries were constructed from testis, ovary, brain and gill rainbow trout tissue samples, and these different libraries were sequenced in 10 separate half-runs of 454-Titanium. Overall, we produced a total of 3million quality sequences with an average size of 328bp, representing more than 1Gb of expressed sequence information. These sequences have been combined with all publicly available rainbow trout sequences, resulting in a total of 242,187 clusters of putative transcript groups and 22,373 singletons. To identify the predominantly expressed genes in different tissues of interest, we developed a Digital Differential Display (DDD) approach. This approach allowed us to characterize the genes that are predominantly expressed within each tissue of interest. Of these genes, some were already known to be tissue-specific, thereby validating our approach. Many others, however, were novel candidates, demonstrating the usefulness of our strategy and of such tissue-specific resources. This new sequence information, acquired using NGS 454-Titanium technology, deeply enriched our current knowledge of the expressed genes in rainbow trout through the identification of an increased number of tissue-specific sequences. This identification allowed a precise cDNA tissue repertoire to be characterized in several important rainbow trout tissues. The rainbow trout contig browser can be accessed at the following

  5. Contribution à l'étude de la "taille" des roches Contribution to the Study of Rock Cutting

    Directory of Open Access Journals (Sweden)

    Nguyenminh D.

    2006-11-01

    Full Text Available Une étude expérimentale détaillée du processus local de destruction des roches par un outil de forage pétrolier a été faite par une méthode de similitude utilisant des matériaux équivalents tendres. On examine l'influence de la forme de l'outil, de la rugosité du contact et de la ductilité e globale » du matériau. Les résultats obtenus sont ensuite comparés à ceux de l'analyse plastique par la théorie des charges limites. Cette étude met en évidence l'existence de deux modes de rupture, l'action différente du frottement superficiel selon le sens de l'écoulement relatif du matériau en contact, le phénomène de la ductilité apparente qui dépend de la forme de l'outil ; elle précise les paramètres mécaniques importants du forage. A detailed experimental investigation of the local process of rock cutting by a drill bit was carried out on soft equivalent materials simulating rock under borehole conditions. Different factors were analyzed : the shape of the bit tooth, the roughness of ifs contact area, the average ductility of the materiat. The results obtained are then compared with those from a plastic analysis based on the peak lood theory. This investigation shows that there are two different modes of rock destruction, that the Coulomb friction has a different effect depending on the relative direction of flow of the materiat in contact with the bit, and that an apparent ductility of the materiat is linked to the shape of the bit teeth. Finally, the basic mechanical variables for drilling are described

  6. Multicenter evaluation of the new Abbott Realtime assays for quantitative detection of human immunodeficiency virus type 1 and hepatitis C virus RNA

    NARCIS (Netherlands)

    M. Schutten (Martin); D. Peters (D.); N. Back (Nicole); A.W. van den Beld (Annewieke); B. Beuselinck (B.); V. Foulongne (V.); A.M. Geretti (Anna Maria); L. Pandiani (L.); M. Tiemann; H.G.M. Niesters (Bert)

    2007-01-01

    textabstractThe analytical performances of the new Abbott RealTime hepatitis C virus (HCV) and human immunodeficiency virus type 1 viral load assays were compared at nine laboratories with different competitor assays. These included the Abbott LcX, Bayer Versant bDNA, Roche COBAS Amplicor, and Roche

  7. Multicenter evaluation of the new Abbott RealTime assays for quantitative detection of human immunodeficiency virus type 1 and hepatitis C virus RNA

    NARCIS (Netherlands)

    Schutten, M; Peters, D; Back, N K T; Beld, M; Beuselinck, K; Foulongne, V; Geretti, A-M; Pandiani, L; Tiemann, C; Niesters, H G M

    2007-01-01

    The analytical performances of the new Abbott RealTime hepatitis C virus (HCV) and human immunodeficiency virus type 1 viral load assays were compared at nine laboratories with different competitor assays. These included the Abbott LcX, Bayer Versant bDNA, Roche COBAS Amplicor, and Roche COBAS TaqMa

  8. Alteration hydrothermale et deformation ductile des roches volcaniques acides associees au gisement sulfure de draa sfar (Jebilet Centrales, Maroc

    Directory of Open Access Journals (Sweden)

    Zinbi, Y.

    2005-12-01

    Full Text Available The volcanics and volcanoclastic rocks of Draa Sfar (Central Jebilet, Moroccan hercynian belt are affected by ductile stress and hydrothermal alteration accompanied by a weak degree of metamorphism (greenschist facies. Some N-S oriented shearing zones, affect locally these formations while being the site of an important hydrothermal activity. The consequences of these transformations from a non to slightly- deformed rhyodacite, show that through these ductile shearing zones: (1 the mineralogical assemblage of hydrothermal alteration is essentially formed by chlorite, sericite, quartz and magnetite; (2 the gradual increase of the alteration indexes is accompanied by the destruction of the phenocrists and the recrystallization of the matrix by phyllosilicates and quartz; (3 the progressive transfer of material is more intense in the more deformed zones where the values of Ti, Al and Zr remain constant. These shearing zones played a very important role in the circulation of fluids and the transformation of the rhyodacite of Draa Sfar.Les roches volcaniques et volcanoclastiques de Draa Sfar (Jebilet centrales, Maroc hercynien sont affectées par une déformation ductile accompagnée d’un métamorphisme de faible degré (faciès schistes verts et d’une altération hydrothermale. Des zones de cisaillement de direction N-S, ont affecté localement ces formations tout en étant vecteurs d’une importante activité hydrothermale. Le suivi de ces transformations à partir de la rhyodacite non ou peu déformée, montre qu’à travers ces zones de cisaillements ductiles : (1 l’assemblage minéralogique d’altération hydrothermale est formé essentiellement de chlorite, de séricite, de quartz et de magnétite ; (2 l’augmentation graduelle des indices d’altération s’exprime par la destruction des phénocristaux au profit d’une matrice recristallisée en phyllosilicates et quartz ; (3 le transfert progressif de la matière est plus intense

  9. Gyrotropy and Anisotropy of Rocks: Similarities and Differences Gyrotropie et anisotropie des roches : similitudes et différences

    Directory of Open Access Journals (Sweden)

    Chichinina T. I.

    2006-12-01

    Full Text Available The main features of wave propagation in gyrotropic media are compared with wave propagation in anisotropic media. The results of numerical modelling are presented for three typical seismic exploration models. The first two models are azimuthally anisotropic media (of orthorombic symmetry system, group 2m without and with gyration. The third model is a gyrotropic transversely isotropic medium with a vertical symmetry axis. The computations have been made for propagation of shear waves along the vertical symmetry axis. For sufficiently short wave paths (in our models less than 400 m the two-component (x, y seismograms are similar for all three models. For longer paths both signal shape and signal duration for the first and the third model differ noticeably. Some evidence (experimental data and a micromodel is given to show that the gyrotropy of rocks does exist or, at least, can exist. Les principales caractéristiques de la propagation des ondes dans les milieux gyrotropes sont comparées avec la propagation des ondes dans les milieux azimutalement anisotropes. Les résultats d'une modélisation numérique sont présentés pour trois modèles caractéristiques d'exploration sismique. Les deux premiers modèles sont des milieux anisotropes (de symétrie orthorhombique, groupe 2m avec et sans gyrotropie. Le troisième modèle est un milieu gyrotrope transverse isotrope avec un axe de symétrie vertical. Ces calculs ont été réalisés pour la propagation des ondes transversales le long de l'axe de symétrie vertical. Pour des trajets sismiques suffisamment courts (pour nos modèles, moins de 400 m, les sismogrammes à deux composantes (x, y sont similaires pour les trois modèles. Pour des trajets plus longs, la forme et la durée du signal diffèrent sensiblement pour les modèles 1 et 3. Ceci a pour but de montrer (à l'aide des données expérimentales et d'un micromodèle que la gyrotropie dans les roches existe, ou, tout au moins, peut

  10. Robe's restricted problem of 2+2 bodies when the bigger primary is a Roche ellipsoid and the smaller primary is an oblate body

    Science.gov (United States)

    Kaur, Bhavneet; Aggarwal, Rajiv

    2014-01-01

    In this problem, one of the primaries of mass m 1 is a Roche ellipsoid filled with a homogeneous incompressible fluid of density ρ 1. The smaller primary of mass m 2 is an oblate body outside the Ellipsoid. The third and the fourth bodies (of mass m 3 and m 4 respectively) are small solid spheres of density ρ 3 and ρ 4 respectively inside the Ellipsoid, with the assumption that the mass and the radius of the third and the fourth body are infinitesimal. We assume that m 2 is describing a circle around m 1. The masses m 3 and m 4 mutually attract each other, do not influence the motions of m 1 and m 2 but are influenced by them. We have extended the Robe's restricted three-body problem to 2+2 body problem under the assumption that the fluid body assumes the shape of the Roche ellipsoid (Chandrashekhar in Ellipsoidal figures of equilibrium, Chap. 8, Dover, New York, 1987). We have taken into consideration all the three components of the pressure field in deriving the expression for the buoyancy force viz (i) due to the own gravitational field of the fluid (ii) that originating in the attraction of m 2 (iii) that arising from the centrifugal force. In this paper, equilibrium solutions of m 3 and m 4 and their linear stability are analyzed. We have proved that there exist only six equilibrium solutions of the system, provided they lie within the Roche ellipsoid. In a system where the primaries are considered as Earth-Moon and m 3, m 4 as submarines, the equilibrium solutions of m 3 and m 4 respectively when the displacement is given in the direction of x 1-axis or x 2-axis are unstable.

  11. Roche and IAEA announce joint initiative to train healthcare workers for Africa's fight against cancer. EDUCARE partnership to launch IAEA's VUCCnet training networks

    International Nuclear Information System (INIS)

    Full text: Roche and the International Atomic Energy Agency (IAEA) announced today the launch of the EDUCARE (EDUcation for Cancer in African REgions) project to provide concerted support to help combat the growing cancer epidemic in sub-Saharan Africa. The EDUCARE project is to be piloted in Ghana, Tanzania, Uganda and Zambia, and is linked with the IAEA's wider initiative to build regional training networks in cancer control and a Virtual University for Cancer Control (VUCCnet) in Africa. A core component for the successful fight against cancer in any country is the education and training of health care providers. The VUCCnet will allow for training to be provided in an integrated and sustainable way in Africa by taking advantage of low-cost online learning tools. The IAEA is working in collaboration with the World Health Organization (WHO) and other international partners to develop the VUCCnet across Africa. The EDCUARE project will facilitate a first-of-its-kind exchange of knowledge and skills, both at the healthcare provider and country-wide level. Training will be provided by an on-line training resource centre, known as the Virtual University for Cancer Control (VUCC), the first such platform for health workers across the continent. Maturin Tchoumi, General Manager Roche South Africa said: 'As a leader in oncology, Roche believes that its strengths, expertise and resources can be used to improve the quality of oncology training and education in the poorest countries in the world. There is a real lack of basic education in oncology in Africa. By contributing our skills and competencies on the ground, Roche can make a real and sustainable improvement.' This new public-private partnership reflects a shared concern over the increasing cancer burden in sub-Saharan Africa, a region of the world where cancer rates are growing rapidly. Cancer now accounts for 12.5% of all deaths worldwide, more than HIV/AIDS, TB and malaria combined. By 2020, there are expected to

  12. Method comparison of the Ortho Vitros Fusion 5,1 chemistry analyzer and the Roche COBAS Integra 400 for urine drug screen testing in the emergency department.

    Science.gov (United States)

    Johnson-Davis, Kamisha L; Thompson, Catherine D; Clark, Chantry J; McMillin, Gwen A; Lehman, Christopher M

    2012-06-01

    Exposure to drugs and toxins is a major cause for the rising number of emergency department visits each year. Immunoassays are commonly used in the emergency department to provide rapid turnaround time for acute care. The purpose of this study was to compare two automated immunoassay chemistry analyzers to determine which platform produced the fewest number of false positive/negative results. Residual patient urine samples were were collected for each of the following drugs/drug classes: cocaine (n = 40), opiates (n = 45), and amphetamines (n = 54) and confirmed either positive or negative by mass spectrometry. Split sample analyses of these specimens were performed on both the Roche COBAS INTEGRA 400 plus and Ortho Vitros 5,1 FS instruments. The results from the two chemistry analyzers were compared to confirmed results. Both immunoassays were prone to false positive results for cocaine and false negative results for opiates and amphetamines. The Vitros Fusion analyzer generated fewer false positive and false negative results for opiate and amphetamine testing than the Roche Integra, but the platforms performed comparably for cocaine.

  13. Aprovechamiento del salto de Bourg-Lés-Valence. La Presa de la Roche de Glun-Drôme – Francia

    Directory of Open Access Journals (Sweden)

    Française d´Enterprise, Compagnie

    1971-05-01

    Full Text Available To take the fullest advantage of the hydroelectric and agricultural usefulness of the Rhône, the Compagnie Nationale du Rhône has divided the river into a number of sections, and these have been separately developed. The hydraulic gradient at La Roche de Glun, at the end of the central section, involves a hydraulic system consisting of a reservoir, a dam and dykes, a canal, a power station, an overflow canal and drainage works in the , surrounding zones. This project has been carried out by the Compagnie Française d'Enterprises.Para explotar al máximo las posibilidades hidroeléctricas y agrícolas del Ródano, la Compagnie Nationale du Rhône ha dividido dicho río en una serie de tramos para proceder a su distribución. El salto de La Roche de Glun, último del tramo central, forma un complejo hidráulico que consta de: un embalse cerrado por diques, una presa, un canal de alimentación, una central, una esclusa, un canal de descarga y unas obras anejas de drenaje para evitar inundaciones en las ciudades próximas. Las obras han sido realizadas por la Compagnie Française d'Entreprises.

  14. Modèle d'anisotropie des propriétés magnétiques induites par l'orientation préférentielle de forme dans une roche déformée

    OpenAIRE

    Vergne, R.; Mollard, P.; Vialon, P.; Rochette, P.

    1988-01-01

    Le but essentiel de ce travail est de montrer comment il est possible, à l'aide d'arguments simples : de justifier les corrélations observées entre l'anisotropie des propriétés magnétiques d'une roche et sa déformation interne et de les chiffrer dans le cas particulier de l'O.P.F. Pour modéliser une roche polyminérale, on considère l'ensemble des grains d'un même minéral : les marqueurs et le reste de la roche : la matrice. On applique à cet ensemble le modèle hydrodynamique en se bornant au ...

  15. A propos du dernier livre de Sebastian Roché, Police de proximité, Nos politiques de sécurité, Paris, Seuil, 2005.

    Directory of Open Access Journals (Sweden)

    Olivier Hassid

    2006-01-01

    Full Text Available Les émeutes urbaines de novembre 2005 viennent de relancer le débat sur la nécessité de disposer d’une police de proximité. Mise en place par Jean-Pierre Chevènement et stoppée par Nicolas Sarkozy, la réforme de la police de proximité est, pour de nombreux observateurs, indispensable au maintien du pacte républicain. La sortie du livre de Sebastian Roché est, en ce sens, une aubaine pour le décideur public. L’objectif de ce livre est simple : éclairer celui-ci sur les causes de l’échec de cet...

  16. Roche tomography of cataclysmic variables -- II. Images of the secondary stars in AM Her, QQ Vul, IP Peg and HU Aqr

    CERN Document Server

    Watson, C A; Rutten, R G M; Schwope, A D

    2003-01-01

    We present a set of Roche tomography reconstructions of the secondary stars in the cataclysmic variables AM Her, QQ Vul, IP Peg and HU Aqr. The image reconstructions show distinct asymmetries in the irradiation pattern for all four systems which can be attributed to shielding of the secondary star by the accretion stream/column in AM Her, QQ Vul and HU Aqr, and increased irradiation by the bright spot in IP Peg. We use the entropy landscape technique to derive accurate system parameters (M1, M2, i and gamma) for the four binaries. In principle, this technique should provide the most reliable mass determinations available, since the intensity distribution across the secondary star is known. We also find that the intensity distribution can systematically affect the value of gamma derived from circular orbit fits to radial velocity variations.

  17. De Sébastien Roch à L’agneau chaste ou de la construction à la déconstruction de la matrice romanesque de la pédophilie

    Directory of Open Access Journals (Sweden)

    Szczur Przemysław

    2015-12-01

    Full Text Available L’obiettivo del presente articolo è di paragonare l’immagine della pedofilia in due romanzi : Sébastien Roch di Octave Mirbeau e L’agneau chaste di Franck Varjac. Mentre il primo ha contribuito a costruire la matrice romanzesca della pedofilia, il secondo prova a decostruirla.

  18. De Sébastien Roch à L’agneau chaste ou de la construction à la déconstruction de la matrice romanesque de la pédophilie

    OpenAIRE

    Szczur Przemysław

    2015-01-01

    L’obiettivo del presente articolo è di paragonare l’immagine della pedofilia in due romanzi : Sébastien Roch di Octave Mirbeau e L’agneau chaste di Franck Varjac. Mentre il primo ha contribuito a costruire la matrice romanzesca della pedofilia, il secondo prova a decostruirla.

  19. Performance evaluation of Roche LightCycler Nano fluorogenic quantitative PCR instrument%罗氏LightCyclerNano荧光定量PCR仪性能评价

    Institute of Scientific and Technical Information of China (English)

    张宇; 李冬; 戴燕; 郑为超; 万海英

    2015-01-01

    目的:对美国罗氏公司LightCycler Nano 32孔荧光定量聚合酶链反应(PCR)仪的主要性能指标进行评价。方法按照美国临床实验室标准化协会(CLSI)制定的评价标准,评价仪器精密度、准确度、灵敏度、可报告范围、仪器间比对等指标。结果 LightCycler Nano 精密度批内变异系数(CV )为2.63%、1.51%,批间 CV 为6.2%、4.15%;准确度与室间质控物比较,5个标本均在靶值区间内;灵敏度检测 CV≤10%;可报告范围为最大稀释比例1∶100;与比对仪器的比对结果偏差结果均小于15%。结论罗氏LightCycler Nano荧光定量PCR仪5项性能经验证后与厂家提供的性能参数相符,可以用于临床检测。%Objective To evaluate the major performance indexes of American Roche LightCycler Nano 32 holes fluorogenic quantitative PCR instrument .Methods According to the evaluation standard formulated by the Clinical and Laboratory Standars Institute(CLSI) ,the precision ,accuracy ,sensitivity ,reportable range and compari‐son between the instruments were evaluated .Results The coefficients of variation(CV) for within‐run assays were 2 .63% and 1 .51% respectively ;CV for between‐run assays were 6 .2% and 4 .15% respectively ;comparing the accu‐racy with the between‐run quality control material ,the five samples were within the target value range;CV for sensi‐tivity was ≤ 10% ;the reportable range of the maximum dilution was 1∶100;the bias of the comparison results with the comparative instrument was less than 15% .Conclusion The verified 5 items of performance in the Roche Light‐Cycler Nano fluorogenic quantitative PCR instrument conform to the performance parameters provided by the manu‐facturer ,indicating that this instrument can be applied in clinical testing in our laboratory .

  20. Diffusion Properties of Carbonated Caprocks from the Paris Basin Propriétés diffusives de roches de couverture carbonatées du Bassin de Paris

    Directory of Open Access Journals (Sweden)

    Berne P.

    2009-11-01

    carbonated caprocks (regardless of the problem of alteration by acidified water, not addressed here. Lastly, a few simple rules for extending the results presented here to other experimental conditions are proposed. L’efficacite d’un stockage geologique de CO2 repose sur les proprietes de confinement des roches de couverture. Parmi ces proprietes, les caracteristiques diffusives jouent un role important qui est parfois sous-estime. En effet, la diffusion est un processus lent mais qui peut s’averer non negligeable sur le long terme. De plus, les caracteristiques recherchees pour une roche de couverture, principalement une forte pression d’entree et une faible permeabilite, ne sont pas incompatibles avec des coefficients de diffusion relativement eleves, puisque ces grandeurs sont liees a des caracteristiques geometriques differentes (schematiquement, les tailles de pore pour les unes et la tortuosite pour les autres. Dans le cadre du projet Geocarbone–INTEGRITE, finance par l’ANR de 2006 a 2008, on a donc mesure les coefficients de diffusion de l’eau et de l’ion bicarbonate dans des echantillons de roche de couvertures carbonatees provenant de trois niveaux (formations des Calcaires du Comblanchien et de la Dalle Nacree. Ces deux especes permettent en effet de couvrir, en premiere approximation, les situations rencontrees en conditions de stockage (espece neutre/espece chargee, interagissant ou non avec la matrice solide. Dans un premier temps, on presente le modele simple de diffusion utilise pour le depouillement des experiences, ce qui permet de passer en revue les differentes grandeurs utiles : coefficient de diffusion effectif, coefficient de diffusion de pore, coefficient de diffusion apparent, porosite accessible, facteur de retard. Le coefficient d’autodiffusion de l’eau est mesure par deux techniques differentes (Resonance Magnetique Nucleaire et tracage radioactif a l’eau tritiee 1H3HO dans deux echantillons. Les resultats obtenus sont

  1. Spoon-Feeding Giant Stars to Supermassive Black Holes: Episodic Roche Lobe Overflow from Evolving Stars and Their Contribution to the Quiescent Activity of Galactic Nuclei

    CERN Document Server

    MacLeod, Morgan; Grady, Sean; Guillochon, James

    2013-01-01

    Stars may be tidally disrupted if, in a single orbit, they are scattered too close to a supermassive black hole (SMBH). Tidal disruption events are thought to power luminous but short-lived accretion episodes that can light up otherwise quiescent SMBHs in transient flares. Here we explore a more gradual process of tidal stripping where stars approach the tidal disruption radius by stellar evolution while in an eccentric orbit. After the onset of mass transfer, these stars episodically overflow their Roche lobes every pericenter passage giving rise to low-level flares that repeat on the orbital timescale. Giant stars, in particular, will exhibit a runaway response to mass loss and "spoon-feed" material to the black hole for tens to hundreds of orbital periods. In contrast to full tidal disruption events, the duty cycle of this feeding mode is of order unity for black holes with mass greater than approximately 10 million solar masses. This mode of quasi-steady SMBH feeding is competitive with indirect SMBH feed...

  2. Total Vitamin D Assay Comparison of the Roche Diagnostics “Vitamin D Total” Electrochemiluminescence Protein Binding Assay with the Chromsystems HPLC Method in a Population with both D2 and D3 forms of Vitamin D

    OpenAIRE

    Gemma Patras; Louisa Grundy; Fasila Pallinalakam; Nafiz Hussein; Faten Mustafa; Arwa Salem; Shoukat Khan; Andrew Turner; Afrozul Haq; Laila Abdel-Wareth; Jaishen Rajah

    2013-01-01

    This study compared two methods of assaying the 25-hydroxylated metabolites of cholecalciferol (vitamin D3) and ergocalciferol (vitamin D2). A fully automated electrochemiluminescence assay from Roche Diagnostics and an HPLC based method from Chromsystems were used to measure vitamin D levels in surplus sera from 96 individuals, where the majority has the D2 form of the vitamin. Deming regression, concordance rate, correlation and Altman Bland agreement were performed. Seventy two subjects (7...

  3. Un habitat orné en abri sous-roche au Magdalénien Moyen, Angles-sur-l'Anglin (Vienne, France

    Directory of Open Access Journals (Sweden)

    Iakovleva, Ludmila

    1999-06-01

    Full Text Available Not Available.

    [fr] L'originalité du site du Roc-aux-Sorciers à Angles-sur l´Anglin dans la Vienne (France se traduit par la présence d'un art pariétal magdalénien sculpté dont une partie, disposée sur la paroi du fond de l'abri sous roche, forme une frise sur près de 18 mètres de long. L´ étude, tant thématique que technique ou stylistique, de cet art pariétal in situ démontre une organisation et une construction dans ce complexe figuratif sculpté. Les sujets, aussi bien dans leur traitement que dans leur rendu, répondent à des règles précises propres au site d'Angles. Ils se regroupent par espèce en s'associant à d'autres sujets animaliers ou humains dans des espaces nettement délimités formant ainsi une composition sculptée. Des zones figuratives rassemblant plusieurs de ces compositions sculptées assimilées à des "panneaux", sont mises en relation avec le positionnement de grands foyers installés sur la roche-mère. Cette analyse nous amène à mettre en valeur une structure complexe d'habitat en liaison avec cette frise pariétale. [es] Un hábitat decorado del Magdaleniense Medio en el abrigo de Angles-sur-l'Anglin (Vienne, France. La originalidad del yacimiento de Roc-aux-Sorciers en Angles-sur-l 'Anglin, en Vienne (Francia, se debe a la existencia de un arte parietal magdaleniense esculpido del que una parte, situada en la pared del fondo del abrigo, forma un friso de 18 m. de largo, aproximadamente. El estudio, tanto temático como técnico o estilístico, de este arte parietal in situ demuestra una organización en este complejo figurativo esculpido. Las figuras, tanto en su tratamiento como en su expresión, responden a reglas precisas, propias del yacimiento de Angles. Se agrupan por especies, asociándose a otras figuras animales o humanas, en unos espacios estrictamente limitados, formando así una composición esculpida. Las zonas figurativas que integran muchas de estas

  4. The Bolivian Source Rocks. Sub Andean Zone. Madre de Dios. Chaco Les roches mères de Bolivie. Subandin. Madre de Dios. Chaco

    Directory of Open Access Journals (Sweden)

    Diaz Martinez E.

    2006-11-01

    connaissances sur les roches mères de Bolivie. Ce travail a été fait dans le cadre de la convention de coopération qui lie depuis 1987 YPFB et l'ORSTOM - Institut Français de Recherche Scientifique pour le Développement en Coopération. L'établissement de cette base de données a permis une synthèse du potentiel pétrolier du pays, la partie concernant le bassin de Madre de Dios, la zone subandine et le Chaco, c'est-à-dire toute la zone potentiellement pétrolière du pays, hors Altiplano, est ici présentée. La base contient actuellement les analyses d'un peu plus de 3000 échantillons. Les roches mères sont Paléozoïques et se sont déposées dans un contexte de bassin d'avant-pays; celui ci s'étendait sur la cordillère ainsi que sur l'avant-pays actuel du Silurien au Permien. Le bassin, restreint initialement à la zone sud-ouest, s'est ensuite étendu vers le nord. La bordure ouest n'est pas connue, elle devait se situer au niveau de l'Altiplano actuel. La bordure est correspond au bouclier Brésilien affleurant actuellement à l'est du pays et sur laquelle se biseaute le Paléozoique. La sédimentation est marine, il s'agit d'une plate-forme carbonatée s'approfondissant au Carbonifère. De nombreuses évidences de diamectites et de figures de resédimentation ont été notées à partir de la fin du Dévonien. Le Trias marque la fin de ces dépôts et se caractérise par une érosion, sans doute due à une phase de rifting avortée. La sédimentation Jurassique et Crétacée est presque toujours continentale et souvent éolienne, il n'y a pas de structuration marquée. Au nord, dans la zone de Madidi, les dépôts du Crétacé terminal (Maestrichian montrent des intercalations marines riches en matière organique (Fm Flora, alors que son équivalent au centre et au Sud (Fm Cajones est purement gréseux. Le soulèvement de la cordillère orientale démarre à la fin de l'Oligocène (27 Ma, Sempere et al. , 1990 et le front de compression se déplace ensuite

  5. ROCHE URYSIS-2400和AIKELAI AX-4030尿液干化学分析仪检测一致性的比较%Comparison on the consistency between ROCHE URYSIS-2400 and AIKELAI AX-4030 urine dry-chemistry analyzers

    Institute of Scientific and Technical Information of China (English)

    吴蕙; 王蓓丽; 郭玮; 潘柏申

    2014-01-01

    目的:比较ROCHE URYSIS-2400尿液干化学分析仪(简称URYSIS-2400)和AIKELAI AX-4030尿液干化学分析仪(简称AX-4030)的检测结果的一致性。方法分别用URYSIS-2400和AX-4030平行测定500例尿液常规标本,比较检测结果的阳性检出率和符合率。随机选取250例使用尿沉渣显微镜复核尿红细胞(ERY)和白细胞(LEU)。通过添加实验评估 URYSIS-2400、AX-4030抗维生素 C 干扰的能力。结果2台仪器比重(SG)差异百分率均值<1%,其余9项干化学检测项目一般符合率都>90%。URYSIS-2400和AX-4030 ERY项目与尿沉渣显微镜计数的符合率分别为82.4%、84.4%,LEU分别为76.0%和83.2%。URYSIS-2400、AX-4030葡萄糖(GLU)和ERY项目添加维生素C浓度达50 mg/dL时不受干扰。结论 URYSIS-2400和AX-4030检测结果一致性良好,但尿液干化学分析仪只是筛检仪器,还应结合显微镜尿沉渣计数复核的结果以满足临床需要。%Objective To compare the consistency of ROCHE URYSIS-2400 and AIKELAI AX-4030 urine dry-chemistry analyzers.Methods A total of 500 urine specimens were detected by the 2 kinds of urine chemistry analyzers in parallel,and the results were analyzed statistically by positive detection rates and accordance rates.A total of 250 cases were randomly selected for reviewing erythrocytes (ERY)and leulcocytes (LEU)by urine sediment microscopy. Experiments by adding vitamin C were used to evaluate URYSIS-2400 and AX-4030 anti-interference abilities against vitamin C.Results The mean difference percentage of specific gravity (SG)between the 2 analyzers was 90%.The consistency rates of URYSIS-2400 and AX-4030 were 82.4% and 84.4% with urine sediment microscopy on ERY,and 76.0% and 83.2% on LEU. URYSIS-2400 and AX-4030 anti-interference abilities against vitamin C were up to 50mg/dL on glucose (GLU)and ERY.Conclusions There is a good consistency between the results of URYSIS-2400 and AX

  6. Etude de l’activité pouzzolanique d’une roche andésitique en Algérie Study of the pozzolanic activity of an andesitic rock in Algeria

    Directory of Open Access Journals (Sweden)

    Clastres P.

    2012-09-01

    Full Text Available Ce travail s’inscrit dans le cadre de l’accord programme Tassili N° 09 MDU 773 entre le Laboratoire de Physicochimie des Matériaux, USTO-Oran et le Laboratoire des Matériaux et Durabilité des Constructions (LMDC, INSA-UPS-Toulouse, France. La présente étude est une contribution aux travaux de la recherche de nouvelles sources d’approvisionnement en ajouts et en matières premières et la valorisation des matériaux naturels locaux utilisés dans la fabrication du ciment. Cette étude porte sur l’activité pouzzolanique des roches volcaniques, elle a pour but de comparer les caractéristiques physiques et mécaniques d’un mortier témoin à ciment de type CEMI 52,5R fourni par la firme Lafarge et d’un mortier à ciment du même type avec ajout dont les taux de substitution de la roche Andésitique est de 0- 40%. Dans notre travail, nous avons utilisé des roches volcaniques appartenant à la famille des Andésites, provenant de la région de Tipaza, Nord Ouest Algérois. L’étude des caractéristiques mécaniques et de l’indice de pouzzolanicité ont montré que les ciments fabriqués sont capables de remplacer certains ciments Portland pour une éventuelle utilisation dans le domaine de construction. This work is a part of the agreement programs Tassili N° 09 MDU 773 between Laboratory of Physic-chemistry Materials, USTO-Oran and Laboratory of Materials and Durability of Constructions (LMDC, INSA-UPS-Toulouse, France. The present study is an attempt to the research of new supply sources in admixtures and raw materials and the evaluation of local natural materials used in the manufacture of cement. It deals with the pozzolanic activity of the volcanic rocks and aims to compare the physical and mechanical characteristics of a mortar control made with cement CEMI 52,5R provided by Lafarge firm and of a mortar made with the same cement incorporated by different replacement rate of andesitic rock in a range of 0 – 40

  7. Mechanical Properties of Rocks: Pore Pressure and Scale Effects Propriétés mécaniques des roches : pression de pore et effets d'échelle

    Directory of Open Access Journals (Sweden)

    Gueguen Y.

    2006-12-01

    Full Text Available Pore pressure plays a major role when considering rocks mechanical properties. In that field, the concept of effective pressure is a key one to deal with fluids mechanical effects. However, its frequent use has been the source of frequent confusing statements. Because of the various meanings which have been attached to that concept, an attempt is made in this paper to clarify it and examine the validity of its various uses relative to rock mechanical behaviour or rock properties. At a macroscopic scale, thermodynamics provides a powerful tool to investigate this. Reversible or irreversible thermodynamics provide general relationships of great interest. But because real rocks are non homogeneous systems, a microscopic approach is also required in order to analyze the mechanical properties from a description of the small scale processes. The microscopic approach is complementary of the macroscopic thermodynamic one as it leads to the calculation of the effective properties of the medium. In this last approach, effective medium theory is a powerful tool. The effective properties as derived from the microscale can be nicely combined to thermodynamic relations to interpret pore fluid pressure effects and scale effects. The example of elastic properties of porous rocks is more specifically emphasized to illustrate this because of both its intrinsic interest and importance as far as applications are concerned. La pression de pore joue un rôle de première importance dans la considération des propriétés mécaniques des roches. Dans ce domaine, le concept de contrainte effective est essentiel pour aborder les effets mécaniques. Toutefois, son utilisation fréquente a conduit à de nombreuses affirmations trompeuses. Compte tenu des significations diverses accordées à ce concept, nous tentons ici de le clarifier et examinons le domaine d'application de ses divers emplois dans le cadre du comportement mécanique ou des propriétés des roches. À l

  8. 罗氏 Co bas c701全自动生化分析仪性能评价%Performance evaluation of Roche Cobas c 701 fully automatic biochemical analyzer

    Institute of Scientific and Technical Information of China (English)

    邓小玲; 侯玉磊; 陈特; 毕小云

    2015-01-01

    Objective To assess the performance of Roche Cobas c701 fully automatic biochemical analyzer. Methods According to EP15‐A2 from Clinical and Laboratory Standards Institute, the electrolyte (potassium, sodi‐um and chloride) and covers all the wavelengths of nine projects (alanine transaminase, aspartate transaminase, alka‐line phosphatase, gamma‐glutamine transaminase, creatinine and urea nitrogen, glucose, total protein, three acyl glyc‐erin) were measured by Roche Cobas c701 analyzer and original reagents. The precisions and accuracies of all parame‐ters were verified. Results In the 2 levels of tested parameters, the standard deviation of repeatability (Sr )was ≤the manufacture′s standard deviation of repeatability (σr ), and the standard deviation of prescision (St ) was≤the manu‐facture′s standard prescision (σt ), the prescision was acceptable and similar to what the manufacter declared. Correla‐tions between theoretic value and actual value were good (regression coefficient was :0. 999 4-1. 000 0). The bias of all parameters was acceptable (within the prescribed scope of CLIA′88)with Roche cobas c701analyzer, compared with the external quality assessment of the ministry of health clinical inspection center. Conclusion The repeatabili‐ty, precision and accuracy of the parameters by Roche Cobas c701 reach the performance that the manufacturer de‐clares.%目的:对罗氏Cobas c701全自动生化分析仪进行性能评价。方法按照美国临床和实验室标准化协会EP15‐A2文件的要求,通过电解质(钾、钠、氯)和涵盖各波长的9个项目(丙氨酸氨基转移酶、天门冬氨酸氨基转移酶、碱性磷酸酶、γ‐谷氨酰转移酶、肌酐、尿素氮、葡萄糖、总蛋白、三酰甘油)对仪器的精密度、准确度、线性范围等进行验证。结果所有检测项目的重复性标准差(Sr)≤厂家声明的标准差(σr)、精密度的标准差(St)≤σt ,均

  9. The Cretaceous Source Rocks in the Zagros Foothills of Iran Les roches mères Crétacé de l'avant-pays du Zagros en Iran : un exemple de bassin intracratonique de grande dimension

    Directory of Open Access Journals (Sweden)

    Bordenave M. L.

    2006-11-01

    Zagros, constituée par les 40 000 km2 du Dezful Embayment , une zone déprimée située dans la province du Khuzistan. Les accumulations d'huile sont associées à un système pétrolier d'âge Crétacé/Tertiaire comprenant six niveaux roches mères d'importance inégale : la partie inférieure de la formation Garau (Néocomien et les formations Gadvan (Barrémien, Ahmadi (Cénomanien inférieur, Gurpi (Sénonien et Pabdeh (Eocène. Ces intervalles sont à l'origine de 99 % des réserves d'huile découvertes à terre en Iran et produites dans deux réservoirs principaux : les calcaires de l'Asmari (Miocène inférieur et du Bangestan (Cénomanien. Ces réservoirs contiennent 330 milliards de barils en places, soit plus de 7 % des réserves mondiales actuelles. Cet article aborde le problème de la distribution des roches mères de l'avant pays iranien et de la partie iranienne du golfe Arabo-Persique. Les facteurs géologiques, océanologiques et climatiques favorisant la mise en place des conditions dysaérobiques et anaérobiques et le dépôt de sédiments riches en matière organique sont discutés. Le rôle de chacune de ces roches mères dans l'approvisionnement des accumulations d'huile est rapidement évoqué. Deux des roches mères Crétacé : la formation Garau déposée au Néocomien dans la région du Luristan, du nord-est du Khuzistan et de la partie nord-est du golfe Arabo-Persique et la formation Kazhdumi, déposée à l'Albien dans la partie centrale du Dezful Embayment, présentent un excellent potentiel pétrolier avec des valeurs initiales (avant maturation thermique de carbone organique pouvant parfois dépasser 10%, des Index d'Hydrogène (IH de l'ordre de 600 mg HC/g C et une épaisseur maximum supérieure à 300 m. La matière organique sédimentaire, d'origine algaire, contient de fortes teneurs en soufre, ce qui la qualifie comme un kérogène de type IIS. Ces roches mères se sont déposées dans les dépressions bien marquées faisant partie

  10. Une méthode précise pour la mise en évidence et l'étude de l'anisotropie dans les roches An Accurate Method for Detecting and Analyzing Anisotropy in Rocks

    Directory of Open Access Journals (Sweden)

    Talebi S.

    2006-11-01

    Full Text Available L'objectif essentiel de cette recherche était de mettre au point une méthode expérimentale pour étudier l'anisotropie de la vitesse des ondes longitudinales (P dans les roches. Cette anisotropie aurait deux causes principales : d'une part, l'existence d'un réseau de pores, de fissures ou de microfissures, distribué de façon anisotrope dans la roche et d'autre part, la structure elle-même, dont l'anisotropie résulte de la nature des minéraux et de leur disposition. Dans un premier temps, nous avons commencé les essais sur des échantillons de carotte, forés dans trois directions perpendiculaires X, Y et Z d'un bloc de deux types de grès : grès des Vosges et grès de Fontainebleau. La vitesse du son a été mesurée sur 3 à 4 sections de carotte avec des intervalles de 30° (6 directions. Elles se distribuent nettement selon une ellipse, appelée l'ellipse d'anisotropie. Ces ellipses ont été tracées par mini-ordinateur et ont permis de mettre en évidence l'état d'anisotropie et l'homogénéité des carottes. La méthode est tellement précise qu'une erreur de repérage des axes sur un échantillon (Y de grès des Vosges a été détectée et certifiée à l'aide de direction des micas. Nous avons effectué le même essai sur les mêmes échantillons saturés sous vide. Une tendance générale à l'augmentation des vitesses, la diminution du degré d'anisotropie (le rapport de Vmax/Vmin et le changement de position de l'axe d'anisotropie a été observée. La comparaison des résultats secs et saturés permet d'avoir une idée de la nature des vides et de leur rôle dans l'anisotropie globale. Dans l'étape suivante de l'étude, nous avons mis au point une méthode de mesure sur les cubes à faces tronquées (66 faces au maximum de différents types de roches (2 grès, 2 marbres, 1 andésite, 1 balsate, et 1 calcaire. Les valeurs de vitesses des ondes (P ont été mesurées et reportées sur un diagramme de Schmidt. Ensuite

  11. 罗氏2010免疫分析仪检测甲胎蛋白的性能验证%Analytical performance of roche 2010 immunoassay system in detecting AFP

    Institute of Scientific and Technical Information of China (English)

    王凡; 蒋红君

    2012-01-01

    目的 探讨罗氏2010免疫分析仪检测甲胎蛋白(AFP)的基本分析性能与质量要求的符合性.方法 参照相关的文件,分别对罗氏2010免疫分析仪检测AFP的精密度、准确度、临床可报告范围和参考区间进行验证.结果 测定均值为11.90 IU/ml的样本,验证后批内精密度为0.28 IU/ml,总精密度为0.45 IU/ml,测定均值为237.23 IU/ml的样本,验证后批内精密度为2.85 IU/ml,总精密度为3.50 IU/ml,均与厂家声明一致;高、低浓度定值质控品检测结果与靶值的相对偏差均小于5%,5份室间质评控制品检测结果与靶值的相对偏倚为-0.41%~6.61%;分析测量范围为0.514~992.7 IU/ml,临床可报告范围为0.514~158 832 IU/ml;参考区间与厂家声明一致.结论 罗氏2010电化学发光免疫分析仪测定AFP的精密度、准确度、分析测量范围性能均与厂家声明一致;此验证方案和实验方法简便易行,可用于其他检验项目的性能验证.%OBJECTIVE To validate the basic performance of Roche 2010 Immunoassay System in detecting fetoprotein (AFP). METHODS The precision, accuracy, reportable range and reference interval of the Roche 2012 Immunoassay System in detecting AFP were analyzed. RESULTS The results of within-run precisions were 0.28 IU/ml of 11.90 IU/ml AFP level and 2.85 IU/ml of 237.23 IU/ml AFP level. The total precisions were 0.45 IU/ml of 11.90 IU/ml AFP level and 3.50 IU/ml of 237.23 IU/ml AFP level. There results were consistent with manufacturer's instruction; The relative bias between the measured results and the target values was less than 5%, and the relative bias between the measured results and the EQA control samples at five levels was -0.41%-6.61%; measurement range was 0.514-992.7 IU/ml within manufacturer's liner range. Clinical re-portable range was 0.514-158 832 IU/ml; Reference interval was 0.0-5.8IU/ml, which was consistent with manufacturer's instruction. CONCLUSION The Roche 2010

  12. [Comparison between the centrifugation on MPA C10 (Roche Diagnostics) and the centrifugation according recommendations of GEHT (Groupe d'étude de l'hémostase et de la thrombose) for the daily hemostasis assays].

    Science.gov (United States)

    Flamant, Fabrice; Borg, Jeanne-Yvonne; Lenormand, Bernard; Le Cam-Duchez, Véronique

    2014-01-01

    Actually, many laboratories tend to acquire pre analytic automates to prepare specimens for analysis. For haemostasis, these pre analytical modules are not always in agreement with the recommendations from the Groupe d'étude de l'hémostase et de la thrombose (GEHT). For example in the MPA C10 module (Roche Diagnostics) the speed of centrifugation was not rather fast compared with the GEHT recommandations. Then, to be able to use this automate for routine coagulation assays, we compared results of Quick time, activated partial prothombin time, fibrinogen, factor II, factor V, factor VII, factor X and antithrombin levels and unfractioned heparin anti-Xa activity measurement after MPA (1,885 g - 999 sec) or GEHT (2,500 g - 900 sec) protocol of centrifugation. First, we verified platelet counts: in 82% of specimens, the platelet counts were under 10.10(9)/L after centrifugation on MPA module. Moreover, a good correlation was observed in all comparisons. Then we concluded the MPA C10 module was usable for routine coagulation tests.

  13. Application of paper chromatography to the determination of some elements in minerals and rocks; Application de la chromatographie sur papier a la determination de certains elements dans les mineraux et les roches

    Energy Technology Data Exchange (ETDEWEB)

    Agrinier, H. [Commissariat a l' Energie Atomique, Fontenay-aux-Roses (France). Centre d' Etudes Nucleaires

    1964-11-01

    This publication describes our work in applying paper chromatography to the fields of mineralogy and geology. We have studied the separation and determination of the following elements: Nb, Ta, Ti, Co, Cu, Mn, Sn, Ge, As, Sb, Bi, U, Th, Be, Au, Pb, Zn, V and Mo. The development of separation methods for these various elements has led us to solve many interference problems. These methods make it possible to separate and measure mineral components. They also enable us to search for trace elements in ores, rocks soils and carbonaceous materials. Finally let us point out that this technique may equally be used for analysis in very radioactive environment. (author) [French] Dans cette etude, nous etendons le champ d'application de la chromatographie sur papier aux domaines de la mineralogie et de la geologie. Nous avons etudie la separation et la determination des elements suivants: Nb, Ta, Ti, Co, Cu, Mn, Sn, Ge, As, Sb, Bi, U, Th, Be, Au, Pb, Zn, V et Mo. La mise au point des methodes de separation concernant ces differents elements nous a amenes a resoudre de nombreux problemes d'interference. Ces methodes permettent la separation et le dosage des constituants des mineraux, ainsi que la recherche d'elements en traces dans les minerais, les roches, les sols et les materiaux carbonnes. Signalons pour terminer que cette technique peut egalement trouver son utilisation dans le domaine de l'analyse en milieu tres radio-actif. (auteur)

  14. Etude de la distribution des terres rares et des autres éléments en traces dans les roches carbonatées du district minier de Bou-Azzer-Bleïda (Anti-Atlas, Maroc) : conséquences génétiques et variations à l'approche des minéralisations

    OpenAIRE

    Zouita, Fouzia

    1986-01-01

    Différents types de roches carbonatées se trouvent dans les formations géologiques précambriennes du district minier de Bou-Azzer-Bleïda. Il s'agit de filons hydrothermaux ou de niveaux sédimentaires. L'utilisation de différentes méthodes d'analyses (dont la Spectrométrie de Masse à étincelles) permet de dresser l'inventaire des éléments majeurs et en traces y compris les Terres Rares dans ces différentes roches carbonatées. L'étude particulière de la répartition des Terres Rares dans ces mat...

  15. Grouped frequent sequential patterns derived from terrestrial image time series to monitor landslide behaviour - Application to the dynamics of the Sanières/Roche Plombée rockslide.

    Science.gov (United States)

    Péricault, Youen; Pothier, Catherine; Méger, Nicolas; Trouvé, Emmanuel; Vernier, Flavien; Rigotti, Christophe; Malet, Jean-Philippe

    2016-04-01

    Image time series acquired with remote sensing methods based on optical terrestrial photogrammetry have great potential for understanding and monitoring the Earth surface dynamics at local scale, and are particularly interesting for landslide monitoring. Image correlation techniques can be applied to calculate the displacement fields, in either the image geometry or the terrain geometry if orthorectification procedures are applied. The resulting products are times series of displacement vectors for each epoch in which knowledge extraction techniques can be applied to discover relevant movement patterns in space and time. We used an unsupervised method (Grouped Frequent Sequential patterns / GFS-patterns) based on the mining of the displacement field. The method was originally developed for the analysis of time series of satellite images. It involves the extraction of trends / sub-trends affecting each pixel covering at least a minimum surface area and sufficiently connected to each other. The results of the mining are presented in spatio-temporal location maps (STL-map) of each GFS-pattern. In these maps, the spatial information is given by the pixel locations and the time information is displayed using a color ramp. The method is tested on a time series of 36 optical terrestrial images of the Sanières/Roche Plombée rockslide (South East French Alps) from 28 of July to 1 September 2014. From this series 35 2D displacement fields were calculated for epochs of three days, and the time series of vector magnitude and direction were analysed with GFS-patterns / STL-map. The method allowed identifying several patterns corresponding to different kinematical behaviour of the rockslide (long-term creep at the top of the slope, surficial movement of the debris at the base of the slope). The unsupervised knowledge extraction method GFS-pattern / STL-map, originally developed to analyse time series of satellite images showed in this study real possibilities of use for

  16. External Quality Assessment Program for Chlamydia trachomatis Diagnostic Testing by Nucleic Acid Amplification Assays

    OpenAIRE

    Land, Sally; Tabrizi, Sepehr; Gust, Anthony; Johnson, Elizabeth; Garland, Susan; Dax, Elizabeth M.

    2002-01-01

    We report the results from 57 Australian diagnostic laboratories testing two external quality assessment panels using either the Roche Amplicor Chlamydia trachomatis test (R-PCR) or the Abbott LCx Chlamydia trachomatis assay (A-ligase chain reaction [LCR]). Panel samples were either normal urine spiked with Chlamydia trachomatis antigen or clinical urine specimens. There was no significant difference between laboratories or between assays in detection of C. trachomatis-positive clinical sampl...

  17. Multicenter Evaluation of the New Abbott RealTime Assays for Quantitative Detection of Human Immunodeficiency Virus Type 1 and Hepatitis C Virus RNA▿

    OpenAIRE

    Schutten, M.; Peters, D.; Back, N.K.T.; Beld, M; Beuselinck, K.; Foulongne, V.; Geretti, A.-M.; Pandiani, L.; Tiemann, C; Niesters, H.G.M.

    2007-01-01

    The analytical performances of the new Abbott RealTime hepatitis C virus (HCV) and human immunodeficiency virus type 1 viral load assays were compared at nine laboratories with different competitor assays. These included the Abbott LcX, Bayer Versant bDNA, Roche COBAS Amplicor, and Roche COBAS TaqMan assays. Two different protocols used during the testing period with and without a pre-m1000 RNA isolation spin were compared. The difference proved to be nonsignificant. A uracil-N-glycosylase (U...

  18. Proposition d'explication de la formation d'hydrogène sulfuré dans les stockages souterrains de gaz naturel par réduction des sulfures minéraux de la roche magasin Proposed Explanation of Hydrogen-Sulfide Formation in Underground Natural-Gas Storage Structures by Reduction of Mineral Sulfides in the Reservoir Rock.

    OpenAIRE

    Bourgeois J. P.; Aupaix N.; Bloise R.; Millet J. L.

    2006-01-01

    La formation d'hydrogène sulfuré dans les structures de stockage peu expliquer autrement que par l'action de bactéries sulfato-réductrices. La contenue dans la roche magasin constitue une source de sulfures capable d'alimenter en H2S le gaz naturel. La réduction de la pyrite en sulfures du type Fe 1-x S et l'équilibre de dissolution précipitation, lié principalement à la pression de CO2, dans les structures stockages, constituent un processus de formation d'H2S capable d'expliquer tativement ...

  19. EUROarray human papillomavirus (HPV) assay is highly concordant with other commercial assays for detection of high-risk HPV genotypes in women with high grade cervical abnormalities.

    Science.gov (United States)

    Cornall, A M; Poljak, M; Garland, S M; Phillips, S; Machalek, D A; Tan, J H; Quinn, M A; Tabrizi, S N

    2016-06-01

    The purpose of this study was to evaluate the performance of the EUROIMMUN EUROArray HPV genotyping assay against the Roche Cobas 4800, Roche HPV Amplicor, Roche Linear Array and Qiagen Hybrid Capture 2 assays in the detection of high-risk HPV (HR-HPV) from liquid based cervical cytology samples collected from women undergoing follow-up for abnormal cervical cytology results. Cervical specimens from 404 women undergoing management of high-grade cytological abnormality were evaluated by EUROarray HPV for detection of HR-HPV genotypes and prediction of histologically-confirmed cervical intraepithelial neoplasia grade 2 or higher (≥CIN2). The results were compared to Hybrid Capture 2, Cobas 4800 HPV, Amplicor and Linear Array HPV. Positivity for 14 HR-HPV types was 80.0 % for EUROarray (95 % CI; 75.7-83.8 %). Agreement (κ, 95 % CI) between the EUROarray and other HPV tests for detection of HR-HPV was good to very good [Hybrid Capture κ = 0.62 (0.54-0.71); Cobas κ = 0.81 (0.74-0.88); Amplicor κ = 0.68 (0.60-0.77); Linear Array κ = 0.77 (0.70-0.85)]. For detection of HR-HPV, agreement with EUROarray was 87.90 % (Hybrid Capture), 93.58 % (Cobas), 92.84 % (Amplicor) and 92.59 % (Linear Array). Detection of HR-HPV was not significantly different between EUROarray and any other test (p < 0.001). EUROarray was concordant with other assays evaluated for detection of high-risk HPV and showed sensitivity and specificity for detection of ≥ CIN2 of 86 % and 71 %, respectively. PMID:27048314

  20. 运用COBAS AMPLICOR HCV系统快速检测HCV RNA

    Institute of Scientific and Technical Information of China (English)

    江志奎; Albad,J

    1999-01-01

    近年来实验室诊断丙型肝炎病毒(HCV)PCR法已向标准化和“即可用”的方向发展。本试验用来自5个检测中心的2000多份标本对首台自动化扩增和检测HCV RNA的仪器COBAS在日常实验室工作中的性能进行了评估。结果表明,自动的COBAS法与AMPLICOR手工法同样准确,符合率达99.8%。PCR法与血清学方法的结果并不完全一致,因为抗HCV抗体的存在有可能表示隐性感染或既往感染。如果以抗HCV抗

  1. Generation of Anomalous Internal Pressures in Source Rocks and Its Role in Driving Petroleum Migration La genèse de pressions internes anormales dans les roches mères et son rôle de poussée dans la migration du pétrole

    Directory of Open Access Journals (Sweden)

    Barker C.

    2006-11-01

    . La présence de gaz conduit à des comportements très différents et la pression de pores dans les roches mères inférieure à la normale à moins qu'il n'y ait une genèse suffisante de la matière organique en hydrocarbures pour la compenser. En l'absence d'une genèse continue (c'est-à-dire pour le méthane biogénique, le gaz se dissout régulièrement et les pressions chutent considérablement en dessous de la pression hydrostatique. A l'inverse, sur les élévations, les systèmes gazéifères ont tendance à la surpression et la pression peut atteindre la charge de fracturation de la roche. Le craquage thermique des bitumes qui restent dans une roche mère au cours de l'enfouissement va de pair avec une augmentation de volume et, dans un pore isolé, le système des pressions atteint des valeurs élevées. Il suffit qu'un pour cent des bitumes de type huile soit transformé en gaz pour que la pression atteigne la charge pour laquelle la fracturation de la roche est une conséquence inévitable.

  2. Impact de la température sur les propriétés mécaniques et acoustiques des roches concernées par la production en SAGD, lors de l'injection de vapeur dans les réservoirs d'huile lourde

    OpenAIRE

    Doan, Dinh Hong

    2011-01-01

    L'injection de vapeur lors de la production des bruts lourds par SAGD soumet les roches réservoirs (sables bitumineux non consolidés ou faiblement consolidés) à une élévation de température (jusqu'à 280°C). L'apport de fluide chaud augmente la pression de pore, dilate le squelette rocheux et le fluide interstitiel, ce qui modifie le champ de contrainte in situ. Le travail de thèse, à forte connotation expérimentale, vise à contribuer à la caractérisation mécanique et acoustique des réservoirs...

  3. Méthode rapide de caractérisation des roches mètres, de leur potentiel pétrolier et de leur degré d'évolution Rapid Method for Source Rocks Characrerysation and for Determination of Petroleum Potential and Degree of Evolution

    Directory of Open Access Journals (Sweden)

    Espitalié J.

    2006-11-01

    Full Text Available Les études fondamentales réalisées sur les kérogènes à l'aide de diverses méthodes physico-chimiques d'analyse ont permis de mettre au point une méthode et un appareillage adaptés à l'exploration pétrolière. On expose dans cet article la mise au point de cette méthode et on montre, à l'aide des paramètres qu'elle permet d'obtenir, ses applications dans le domaine de l'exploration pétrolière - reconnaissance des différents types de roche mère et de leur potentiel pétrolier; - caractérisation de leur degré d'évolution (zone à huile - zone à gaz. On montre aussi que cette méthode convient particulièrement bien à l'estimation du rendement en huile des roches bitumineuses et à l'étude de la qualité et du rang des charbons. Fundamental research on kerogens by different physico-chemical analysis methods have led to the development of a method and equipment suited for petroleum exploration. This article describes the development of this method and, by means of the parameters it determines, shows how it con be applied in the field of petroleum exploration - exploring different types of source rock and their petroleum potential ; - characterizing their degree of evolution (ail zone/gas zone. This method is also shown ta be particularly suited for estimating the oil yield of cil shales and investigating the quality and classification of cool.

  4. Étude de la mouillabilité des roches réservoir à l'échelle du pore par cryomicroscopie électronique à balayage Wettability of Reservoir Rock At the Pore Scale: Contribution of Cryo-Scanning Electron Microscopy

    Directory of Open Access Journals (Sweden)

    Fassi-Fihri O.

    2006-11-01

    Full Text Available Le but de cette étude est de caractériser, à l'échelle du pore, la mouillabilité des roches réservoir, en relation avec leur géométrie et/ou leur minéralogie. Cette caractérisation se fait, après congélation des échantillons, par l'observation de la distribution des fluides au sein du milieu poreux (saumure et huile brute, en microscopie électronique à balayage. Les expériences ont d'abord été effectuées sur des roches modèles parfaitement mouillables à l'eau, verre fritté et grès naturels. Certains de ces minéraux ont été rendus hydrophobes par greffage de silane. L'étude de ces systèmes a mis en évidence une corrélation entre la mouillabilité et la distribution des fluides. Puis, une roche réservoir (grès argileux de la formation de Brent, de mer du Nord connue comme étant de mouillabilité intermédiaire a été étudiée. Un travail précédent (étude de déplacements eau/huile par tomographie X avait abouti à la conclusion que si ces roches présentaient des hétérogénéités de mouillabilité, l'échelle de ces hétérogénéités devait être inférieure au millimètre. Les études de cryomicroscopie ont montré le caractère hydrophobe de la kaolinite, tandis que les illites, le quartz et les feldspaths sont préférentiellement mouillables à l'eau. L'imbibition spontanée d'huile pourrait ainsi être attribuée à l'existence au sein de la roche d'un réseau de kaolinite, tandis que l'imbibition spontanée de saumure serait due à l'existence d'un second réseau plus ou moins imbriqué avec le premier et constitué des autres minéraux. Un autre cas de roche réservoir a été étudié, à savoir un carbonate du Moyen-Orient. Les mésopores intergranulaires y ont été observés comme étant mouillables à l'huile tandis que les micropores restaient mouillables à l'eau. Dans ce cas, la mouillabilité intermédiaire de ces échantillons s'explique par la géométrie plutôt que par la min

  5. Evaluation of Cobas TaqMan MTB PCR for detection of Mycobacterium tuberculosis.

    Science.gov (United States)

    Kim, Jeong Hyun; Kim, Young Jae; Ki, Chang-Seok; Kim, Ji-Youn; Lee, Nam Yong

    2011-01-01

    Nucleic acid-based amplification tests allow the rapid detection of Mycobacterium tuberculosis. Recently, a real-time PCR assay for M. tuberculosis complex, the Cobas TaqMan MTB test (Roche Diagnostics, Basel, Switzerland), was introduced. We performed a prospective study to evaluate the diagnostic performance of the Cobas TaqMan MTB test system. A total of 406 specimens collected from 247 patients were simultaneously tested by conventional culture, Cobas Amplicor MTB PCR, and TaqMan MTB PCR. The cross-reactivity with other Mycobacterium species and the detection limit were also evaluated. Among 406 specimens, a total of 24 specimens (5.9%) were culture positive: 14 specimens were positive by both TaqMan and Amplicor MTB PCRs, while 5 specimens were positive by only TaqMan PCR. The remaining five specimens were negative by both PCR methods. Seven specimens with negative culture results were positive by TaqMan PCR, but five of these were negative by Amplicor MTB PCR. The sensitivity, specificity, and positive (PPV) and negative (NPV) predictive values were 79.1%, 98.2%, 73.1%, and 98.7% for TaqMan and 58.3%, 99.5%, 87.5%, and 97.4% for the Amplicor MTB PCR test, respectively. There was no cross-reactivity with M. tuberculosis and nontuberculous mycobacterial species. The detection limit for the Cobas TaqMan MTB PCR test was 4.0 copies/μl. The Cobas TaqMan MTB PCR test showed higher sensitivity for detection of the M. tuberculosis complex without disturbing the specificity and NPV than the Amplicor MTB PCR test.

  6. 罗氏生化多项定标液(CFAS)在 ADVIA1800自动生化仪上的应用%Roche biochemical multiple calibration solution (CFAS)used in the ADVIA 1800 automatic biochemical discussion on

    Institute of Scientific and Technical Information of China (English)

    周明欣

    2016-01-01

    目的:规范实验室生化项目的参考范围,统一临床检验数值,为临床医师提供更准确、更一致的检验结果,方便患者治疗效果的评价。方法同步于罗氏Cobas C501自动生化仪和ADVIA1800自动生化仪上进行多项目的评价。结果1.定标与校正验证合格;2.正常质控品和异常质控品在可控范围内;3.临床常规标本比对评价没有显著性差异。结论罗氏生化多项定标液(CFAS)在ADVIA1800自动生化仪上的应用效果满意,能为医师提供准确、统一的检验结果,方便医师治疗病人及疗效观察。%Objective to standardize laboratory biochemical reference scope of the project ,unified clinical inspection number , provide clinicians with more accurate and more consistent test results ,convenient patient treatment effect evaluation .Methods the synchronous in roche Cobas C501 automatic biochemistry analyzer and multiple objective evaluation ADVIA 1800 automatic bio‐chemistry analyzer .Results 1 ,the calibration and correction verification qualified ,2 ,normal quality products and quality control within the scope of the controllable ,3 ,there is no significant difference between clinical specimens of normal ratio evaluation .Con‐clusion roche biochemical multiple scaling liquid(CFAS)in the application effect of satisfaction on ADVIA 1800 automatic biochem‐istry analyzer ,for physicians to provide accurate ,unified inspection result ,convenient for doctors treat patients and curative effect observation .

  7. Single-step PCR in molecular diagnosis of hepatitis C virus infection.

    OpenAIRE

    Farma, E; E. Boeri; Bettini, P.; Repetto, C M; McDermott, J.; Lillo, F B; Varnier, O E

    1996-01-01

    The diagnostic utility of two PCR systems and three PCR detection methods for hepatitis C virus (HCV) RNA was evaluated in serum samples. A nested PCR was considered the reference assay and was compared with two single-step PCR methods: the first is based on the detection of PCR products by liquid hybridization with a 32P-end-labeled probe, and the second is the Roche Amplicor colorimetric assay using microwell plate hybridization with a specific nucleic acid probe. Using the Pelicheck HCV RN...

  8. 罗氏 Cobas e601与雅培 Architect i2000检测乙肝表面抗原的比较%Comparison of HBsAg Results between Roche Cobas e601 and Abbott Architect i2000 analysis systems

    Institute of Scientific and Technical Information of China (English)

    孙彬; 李康; 吴纯; 郭奉洁; 白光亮; 董梅

    2014-01-01

    目的:比较罗氏电化学发光检测仪Cobas e601与雅培化学发光微粒子免疫分析仪Architect i2000检测乙肝表面抗原的结果。方法选取在雅培 i2000检测仪上检测乙肝表面抗原结果为有反应性,且定量结果小于250 IU/mL 的标本,同时在罗氏 e601进行检测,分析比较检测结果间的差别,并进行直线相关和回归分析。结果共检测46份临床标本,剔除1份两台仪器上检测结果反应性不符的标本外,其余结果具有很好的相关性。其中雅培检测值为0.05~1.00 IU/mL 的15份,两者的直线回归方程为Y =17.49X +0.843,相关系数 r=0.979;1.1~10.00 IU/mL 的15份,两者的直线回归方程为Y =15.72X +21.06,相关系数 r=0.952;11~250 IU/mL 的15份,两者的直线回归方程为Y =29.17X -129,相关系数 r=1.000。结论两种方法的检测结果具有很好的相关性,并可以通过公式进行相互换算。%Objective To compare the difference of the HBsAg detected results between the Roche cobas e601 electrochemilumi-nescence immunoassay instrument and the Abbott Architect i2000chemiluminescent microparticle immunoassay instrument.Methods The HBsAg positive specimens with the quantitation results of lower than 250IU/mL detected by the Abbott Architect i2000 were selected and simultaneously detected by the Roche cobas e601.The differences of detected results were compared and per-formed the linear correlation and analysis regression.Results 46 clinical specimens were detected.The detected results had best correlation between the two instruments by getting rid of 1 specimen with unconformable reactivity of detected results.15 speci-mens had the HBsAg detected result of 0.05-1.00 IU/mL by the Abbott Architect i2000,the linear regression equation was Y =17.49X+0.843(r=0.979);15 specimens had the HBsAg detected result of 1.1 -10.00 IU/mL IU/mL by the Abbott Architect i2000,the linear

  9. Étude numérique du couplage thermohydromécanique dans les roches. Influence des termes de couplage non linéaires pour un matériau isotrope linéaire Numerical Analysis of a Thermohydromechanical Coupling in Rocks. Influence of Nonlinear Coupling Terms on a Linear Isotropic Material

    Directory of Open Access Journals (Sweden)

    Henry J. P.

    2006-11-01

    Full Text Available Nous présentons dans ce travail une étude numérique basée sur la méthode des éléments finis, du comportement thermoporoélastique de certaines roches. Les trois effets de couplage : déformabilité de la roche, pression interstitielle et température sont pris en compte simultanément dans la résolution numérique. Une application simple sur un puits pétrolier en conditions axisymétriques est finalement présentée afin de dégager en particulier l'influence du terme de couplage convectif non linéaire, obtenu dans l'équation de diffusivité thermique, sur l'évolution de la température et de la pression interstitielle autour du forage. This article describes a finite-element method for solving the problem of nonlinear coupling between interstitial pressure and temperature during stress on a poroelastic rock. Such coupling phenomena occur during massive injection of cold water into a petroleum borehole for example. The implementation of such a numerical solution, used here with the assumption of small deformations, first requires a review of the behavior law of the material (Eq. 2. 2 and of the equations for hydraulic diffusivity (Eq. 2. 3 and thermal diffusivity (Eq. 2. 4. This last equation (2. 4 is the one containing the nonlinear coupling terms in Grad P Grad P and Grad T. Grad P. During simulation of flow at a high flow rate, these products can no longer be neglected as shown by the results in Fig. 2. The variational formulation of the problem is then determined in relation to the three equations for equilibrium, thermal diffusivity and hydraulic diffusivity. After geometric and temporal discretizations, this formulation leads to a finite-element calculating scheme resulting in the simultaneous solving of all three equations. This solution, based on the inversion of the system of equations (2. 15, requires the updating of the rigidity matrix at each time step to take nonlinear coupling into consideration. Calculations with an

  10. Clinical Performance of Roche Cobas 4800 HPV Test

    Science.gov (United States)

    Cui, Miao; Chan, Nicholas; Liu, Momo; Thai, Khanh; Malaczynska, Joanna; Singh, Ila; Zhang, David

    2014-01-01

    Evaluation of the Cobas 4800 test demonstrated that Cobas had a low rate of cross-reactivity with low-risk human papillomavirus (lrHPV), a 3.74% disconcordance rate between prealiquots and postaliquots, and failure rates of 4.57% and 1.16%, respectively, after vortexing and swirling. This study demonstrated that the Cobas test has good sensitivity, accuracy, and reproducibility for detecting 14 high-risk HPV (hrHPV) genotypes. PMID:24719443

  11. In the heart of rock; Au coeur de la roche

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1999-01-01

    This educational document gives a presentation of the Andra underground research laboratory of Bure (Haute-Marne and Meuse, France) devoted to the study of the underground disposal of radioactive wastes. The document is presented in the form of a comics. It describes the geological setting of the laboratory, located in the eastern edge of the Paris basin, the properties of the Callovian-Oxfordian clay layer, the digging of the boreholes and galleries, the mine-by test and its instrumentation, the backfilling and sealing of the galleries and the reversibility of the burial of radioactive waste containers. (J.S.)

  12. Mida roheline tarbija tahab / Catherine Roche ; intervjueerinud Michael S. Hopkins

    Index Scriptorium Estoniae

    Roche, Catherine, 1977-

    2009-01-01

    2008. a. tehtud uuringust, mille abil selgitati välja, mis põhjustel inimesed eelistavad tarbimisel rohelist tootevalikut. Skeemid: Valmisolek maksta rohelise eest rohkem sõltub tootekategooriast ja tajutavatest eelistest; Teadlikkus ja valik

  13. Riemann-Roch Spaces and Linear Network Codes

    DEFF Research Database (Denmark)

    Hansen, Johan P.

    a metric on the set of vector spaces and showed that a minimal distance decoder for this metric achieves correct decoding if the dimension of the intersection of the transmitted and received vector space is sufficiently large. The vector spaces in our construction have minimal distance bounded from below...

  14. Le souffre thiophénique dans les pétroles et les extraits de roche. Analyse par spectrométrie de masse et chromatographie en phase gazeuse Mass-Spectrometry and Gas-Chromatography Analysis of Thiophene Sulfur in Oil and Rock Extracts

    Directory of Open Access Journals (Sweden)

    Castex H.

    2006-11-01

    Full Text Available On étudie par spectrométrie de masse à haute résolution la répartition du soufre thiophénique par coupe de distillation et par nombre de carbone dans la fraction > C10 d'un pétrole brut. Parallèlement on chromatographie à l'aide d'un détecteur sélectif à photométrie de flamme les diverses fractions thiophéniques. L'examen des chromatogrammes permet de déterminer les zones dé réponse et l'allure générale des différentes coupes thiophéniques. Les applications sont variées : on vérifie le bien-fondé de l'utilisation d'un spectromètre de masse à basse résolution pour les analyses en routine d'huiles brutes et on peut, par la connaissance des différents profils de type thiophénique prévoir des analogies ou des différences au sein d'huiles brutes et d'extraits de roche d'un même bassin pétrolier, ce qui limite le nombre d'échantillons à analyser en détail. High-resolution mass spectrometry is used to analyze the distribution of thiophenic sulfur per distillation cut and per carbon number in the > C10 fraction of a crude oil. At the same time, a selective flame-photometry sensor is used to chromatograph the different thiophenic fractions. An analysis of the chromatograms serves to determine the response zones and the general aspect of the different thiophenic cuts. There are various applications of this process. The validity of using a low-resolution mass spectrometer is checked for routine crude-cil analyses. By determining the different profiles of the thiophenic type, similarities or differences can be predicted in the crude cils and rock extracts from the saure cil basin. This limits the number of samples having to be analyzed in detail.

  15. Approche numérique et quantitative de l'étude sur clichés aériens de la fracturation des réservoirs en roches fissurées Numerical and Quantitative Approach to Investigating Fracture Maps of Fissured Reservoirs from Airphotos

    Directory of Open Access Journals (Sweden)

    Razack M.

    2006-11-01

    Full Text Available Dans le cadre de la détermination de la structure des réservoirs en roches fissurées, une méthodologie de traitement numérique et quantitative de la fractu-ration relevée sur clichés aériens a été mise au point. La numérisation du relevé est obtenue par digitalisation sur un lecteur de courbes. Cette information est ensuite traitée à l'aide d'un algorithme (programme RAFRAC qui permet d'identifier numériquement la fracturation par un ensemble de para-mètres élémentaires (orientation, longueur , densité... à partir desquels il devient possible de procéder à une analyse approfondie du champ de fractures considéré. Un exemple d'application est présenté à propos de l'étude de la fracturation d'une zone à structure tabulaire (Causse du Larzac. Ces premiers résultats tendent à mettre en évidence une double nature mathématique de l'information analysée aléatoire d'une part, déterministe d'autre part. On montre également, à partir de la théorie desvariables régionalisées, que la fracturation suit une certaine logique dans sa répartition spatiale. Par ailleurs une organisation des familles de fractures a pu être mise en évidence, sur laquelle la structure géométrique du réservoir sous-jacent s'ajuste de façon très proche. As part of efforts ta determine the structure of reservoirs in fissured rocks, a numerical and quantitative processing methodology has been developed for mapping fractures with airphotos. The survey is digitized on a curve reader. This data is then processed by means of an algorithm (RAFRAC program sa as ta numerically identify fracturing by a set of elementary parameters (direction, length, density, etc. which con be used ta make an in-depth analysis of the fracture field being considered. A sample application is described having ta do with the surveying of the fracturing in a tabular structure (Causse plateau of Larzac. These initial results tend to reveol a dual mathematical nature

  16. Rock Permeability and Fluid Pressure At the Ktb. Implications from Laboratory-And Drill Hole-Measurements Perméabilité des roches et pression dans le KTB : enseignements tirés des mesures de laboratoire et des mesures en puits

    Directory of Open Access Journals (Sweden)

    Zimmermann G.

    2006-12-01

    Full Text Available Rock permeability and the fluid pressure were investigated at different scales at the two drill holes of the Continental Deep Drilling Program (KTB. Drill hole tests and fluid inclusion investigations both implicate the existence of hydrostatic fluid pressure in situ with respect to salinity of the formation fluid. Matrix permeability and in situ values from hydraulic tests differ up to three decades with higher values in situ. Further on, the pressure dependence of core permeability and in situ determined values differ significantly. All these observed effects support the well known theory of scale variance. This conclusion is supported by observations of hydraulic communications between both drill holes. These scale effects implicate a pronounced hydraulic heterogeneity of the KTB surroundings. Therefore, stochastic network modelling with parameters derived from structural borehole measurements and under the consideration of the observed permeabilities were performed. Under the presumption of existing driving forces fluid transport takes place dominantly on discrete connected pathways characterised by fracture width, fracture length and fracture orientation and is subordinate in the rock matrix. La perméabilité des roches et la pression des fluides ont été étudiées à différentes échelles sur les deux forages du Programme continental de forage profond - Continental Deep Drilling Program (KTB. Les essais de puits et les recherches d'inclusions de fluides impliquent l'existence d'une pression de fluide fonction de la salinité du fluide de formation. Les valeurs déduites des essais de puits dépassent largement les perméabilités matricielles, l'écart allant jusqu'à trois ordres de grandeur. De plus, l'évolution de la perméabilité mesurée sur échantillon en fonction de la pression et celle déterminée in situ diffèrent largement. Ces observations renforcent la théorie bien connue des effets d'échelle. Cette conclusion est

  17. Propagation des ondes élastiques dans les matériaux non linéaires Aperçu des résultats de laboratoire obtenus sur les roches et des applications possibles en géophysique Propagation of Elastic Waves in Nonlinear Materials Survey of Laboratory Results on Rock and Geophysical Applications

    Directory of Open Access Journals (Sweden)

    Rasolofosaon P.

    2006-12-01

    Full Text Available Les roches présentent souvent un comportement élastique nettement non linéaire, entraînant des conséquences importantes sur la propagation des ondes. Cette non-linéarité élastique est surtout causée par les microdéfauts mécaniques ubiquistes (microfissures, joints de grains, macles, etc. dont la rigidité varie sous l'effet de la contrainte. Ce sujet fait l'objet d'études de plus en plus nombreuses. Nous nous proposons de présenter très sommairement les bases théoriques et les résultats expérimentaux permettant d'avoir un ordre de grandeur des effets caractéristiques observés dans les roches afin de pouvoir proposer une approche critique des possibilités d'applications en géophysique. Deux disciplines se sont développées en parallèle à partir du même principe physique et avec des formalismes très proches : - L'acousto-élasticité étudie l'effet des précontraintes statiques sur les vitesses de propagation des ondes élastiques. On dispose d'un formalisme mécanique élaboré permettant de relier quantitativement variation de contrainte et variation de vitesse élastique (par exemple pour ce qui concerne l'anisotropie acoustique induite par un état de contrainte et d'une méthode expérimentale de mesure des coefficients de non-linéarité. - L'acoustique non linéaire s'intéresse aux conséquences de la variation des modules élastiques au passage d'une onde qui ne peut plus être considérée comme une petite perturbation, mais qui induit localement des modifications mesurables du milieu de propagation ; modifications entraînant l'apparition de phénomènes inconnus en acoustique linéaire tels que la génération d'harmoniques et l'interaction onde-onde. Les applications à la sismique pétrolière semblent fort lointaines puisque, avec les méthodes classiques de surface ou de puits, il y a peu d'espoir de réussir à faire propager jusqu'aux couches profondes des ondes dont l'amplitude dépasserait le seuil de

  18. Evolution of the Petrophysical and Mineralogical Properties of Two Reservoir Rocks Under Thermodynamic Conditions Relevant for CO2 Geological Storage at 3 km Depth Évolution des propriétés physiques et minéralogiques de deux roches réservoirs dans des conditions thermodynamiques correspondant à un stockage géologique de CO2 à 3 km de profondeur

    Directory of Open Access Journals (Sweden)

    Rimmelé G.

    2009-11-01

    est considérée comme une solution pour contribuer à la réduction des émissions de gaz à effet de serre dans l’atmosphère. Les interactions entre le CO2 supercritique et la roche-réservoir potentielle doivent être étudiées en détail en conditions de stockage géologique. Quarante échantillons de calcaire de Lavoux et de grès d’Adamswiller, provenant de roches réservoirs du bassin parisien, ont été expérimentalement exposés au CO2 dans un autoclave spécialement construit pour reproduire les conditions thermodynamiques d’un réservoir de stockage de CO2. Les deux types de roches ont été exposés pendant un mois à du CO2 supercritique humide et à de l’eau saturée en CO2, à 28 MPa et 90 °C, ce qui correspond à des conditions d’enfouissement de 3 km de profondeur. L’évolution de leurs propriétés minéralogiques a été suivie par des analyses par diffraction des rayons X, par spectroscopie Raman et par microscopie électronique couplée à un système de microanalyses X. Leurs propriétés physiques et microtexturales ont été estimées en mesurant, avant et après les expériences, le poids, la densité, les propriétés mécaniques, la perméabilité, la porosité globale et la présence d’éventuels gradients de porosité de chaque échantillon. Les résultats montrent que les deux roches ont préservé leurs propriétés mécaniques et minéralogiques, malgré une augmentation de la porosité et de la perméabilité. Des zones microscopiques de dissolution de la calcite observées dans le calcaire sont vraisemblablement responsables de cette augmentation de la perméabilité et de la porosité. Dans le grès, une altération de la pétro-fabrique est supposée se produire due à la réaction des minéraux argileux avec le CO2. Tous les échantillons du calcaire de Lavoux et du grès d’Adamswiller ont montré une altération mesurable dans le CO2 humide et dans l’eau chargée en CO2. Ces expériences en autoclave sont

  19. Impact of HIV-1 genetic diversity on plasma HIV-1 RNA Quantification: usefulness of the Agence Nationale de Recherches sur le SIDA second-generation long terminal repeat-based real-time reverse transcriptase polymerase chain reaction test.

    Science.gov (United States)

    Rouet, François; Chaix, Marie-Laure; Nerrienet, Eric; Ngo-Giang-Huong, Nicole; Plantier, Jean-Christophe; Burgard, Marianne; Peeters, Martine; Damond, Florence; Ekouevi, Didier Koumavi; Msellati, Philippe; Ferradini, Laurent; Rukobo, Sandra; Maréchal, Valérie; Schvachsa, Nilda; Wakrim, Lahcen; Rafalimanana, Christian; Rakotoambinina, Benjamin; Viard, Jean-Paul; Seigneurin, Jean-Marie; Rouzioux, Christine

    2007-08-01

    The high genetic diversity of HIV-1 has a major impact on the quantification of plasma HIV-1 RNA, representing an increasingly difficult challenge. A total of 898 plasma specimens positive for HIV-1 RNA by commercial assays (Amplicor v1.5; Roche Diagnostic Systems, Alameda, CA or Versant v3.0; Bayer Diagnostics, Emeryville, CA) were tested using the Agence Nationale de Recherches sur le SIDA second-generation (G2) real-time reverse transcriptase polymerase chain reaction (RT-PCR) test: 518 samples containing HIV-1 of known subtype, including 88 from 2 subtype panels and 430 harboring B (n = 266) and non-B (n = 164) group M HIV-1 subtypes from patients followed up in 2002 through 2005 at Necker Hospital (Paris, France), and 380 samples from 10 different countries (Argentina, Cambodia, Cameroon, Central African Republic, France, Ivory Coast, Madagascar, Morocco, Thailand, and Zimbabwe). HIV-1 RNA values obtained by G2 real-time PCR were highly correlated with those obtained by the Amplicor v1.5 for B and non-B subtypes (R = 0.892 and 0.892, respectively) and for samples from diverse countries (R = 0.867 and 0.893 for real-time PCR vs. Amplicor v1.5 and real-time PCR vs. Versant v3.0, respectively). Approximately 30% of specimens harboring non-B subtypes were underquantified by at least -0.51 log10 in Amplicor v1.5 versus 5% underquantified in G2 real-time PCR. Discrepant results were also obtained with subtype B samples (14% underquantified by Amplicor v1.5 vs. 7% by G2 real-time PCR). Similar percentages were observed when comparing results obtained with the G2 real-time PCR assay with those obtained using the Versant assay. Addressing HIV-1 diversity, continual monitoring of HIV-1 RNA assays, together with molecular epidemiology studies, is required to improve the accuracy of all HIV RNA assays.

  20. Impact of Diagenetic Alterations on the Petrophysical and Multiphase Flow Properties of Carbonate Rocks Using a Reactive Pore Network Modeling Approach Impact des altérations diagénétiques sur les propriétés pétrophysiques et d’écoulement polyphasique de roches carbonates en utilisant une modélisation par l’approche réseau de pores

    Directory of Open Access Journals (Sweden)

    Algive L.

    2012-02-01

    replacing the original complex pore structure of real porous media by a conceptual network. The second step consists of resolving the governing equations of the precipitation and dissolution phenomena (i.e. reactive convection diffusion equation in the conceptual 3D pore network and deducing the local reactive fluxes and the motion of the fluid-solid interface. The third step consists of updating the new pore structure and calculating the new petrophysical properties of the modified porous media. Those steps are repeated in order to mimic a given diagenetic scenario. Finally, the multiphase flow properties of the current porous media are calculated. The impact of one diagenetic cycle of dissolution and precipitation on the pore networks’ heterogeneity and consequently on the petrophysical properties (i.e. porosity and permeability and multiphase flow properties (i.e. relative permeability and capillary pressure have been investigated. The permeability and porosity evolution during a given diagenetic cycle are calculated and analyzed as a function of the relevant dimensionless numbers (Peclet and Damköhler numbers that characterize the flow and reaction regime. The correlation between these numbers and the dissolved/precipitated layer thickness distribution is investigated. This work contributes to improve the understanding of the impact of dissolution and precipitation on permeability and porosity modification. Using the PNM approach, multiphase flow properties and permeability-porosity relationship have been determined for different reactive flow regimes. These relationships are relevant input data to improve the quality of reservoir simulation predictions. Les roches sédimentaires présentent souvent une structure porale hétérogène qui est intrinsèquement liée à la texture de la roche d’origine et aux modifications diagénétiques subies. Ces altérations sont régies par la texture de la roche d’origine, les fluides impliqués (et les interactions rock

  1. Strategy for the maximization of clinically relevant information from hepatitis C virus, RT-PCR quantification.

    LENUS (Irish Health Repository)

    Levis, J

    2012-02-03

    BACKGROUND: The increasing clinical application of viral load assays for monitoring viral infections has been an incentive for the development of standardized tests for the hepatitis C virus. OBJECTIVE: To develop a simple model for the prediction of baseline viral load in individuals infected with the hepatitis C virus. METHODOLOGY: Viral load quantification of each patient\\'s first sample was assessed by RT-PCR-ELISA using the Roche MONITOR assay in triplicate. Genotype of the infecting virus was identified by reverse line probe hybridization, using amplicons resulting from the qualitative HCV Roche AMPLICOR assay. RESULTS: Retrospective evaluation of first quantitative values suggested that 82.4% (n=168\\/204) of individuals had a viral load between 4.3 and 6.7 log(10) viral copies per ml. A few patients (3.4%; n=7\\/204) have a serum viremia less than the lower limit of the linear range of the RT-PCR assay. Subsequent, prospective evaluation of hepatitis C viral load of all new patients using a model based on the dynamic range of viral load in the retrospective group correctly predicted the dynamic range in 75.9% (n=33\\/54). CONCLUSION: The dynamic range of hepatitis C viremia extends beyond the linear range of the Roche MONITOR assay. Accurate determination of serum viremia is substantially improved by dilution of specimens prior to quantification.

  2. The Usefulness of Defining Rapid Virological Response by a Very Sensitive Assay (TMA) during Treatment of HCV Genotype 2/3 Infection

    DEFF Research Database (Denmark)

    Dalgard, Olav; Martinot-Peignoux, Michelle; Verbaan, Hans;

    2015-01-01

    The aim of this study was to determine in patients with HCV genotype 2 or 3 the performance at week 4 of two assays with different sensitivities for HCV RNA detection, for the prediction of SVR and stratification for treatment duration (14 and 24 weeks). Recruitment was from two trials comparing 14...... and 24 weeks treatment to patients with rapid virological response (RVR) (n = 550). RVR was originally defined as HCV RNA HCV-RNA was prospectively...... measured with COBAS Amplicor V2, Roche (CA) (lower detection limit 50 IU/ml) and retrospectively assessed with VERSANT HCV-RNA Qualitative Assay, Siemens (TMA) (lower limit detection 10 IU/ml). Genotype 3 was present in 80% and genotype 2 in 20%. A SVR was achieved in 82%. At week 4 HCV...

  3. Can the Swedish new variant of Chlamydia trachomatis (nvCT) be detected by UK NEQAS participants from seventeen European countries and five additional countries/regions in 2009?

    Science.gov (United States)

    Unemo, M; Rossouw, A; James, V; Jenkins, C

    2009-05-14

    In 2006, a new variant of Chlamydia trachomatis (nvCT) was reported in Sweden. The nvCT contains a deletion that includes the targets for the C. trachomatis genetic diagnostic single-target systems from Roche Diagnostics and Abbott Laboratories. Roche and Abbott have now developed certified dual-target assays that can detect the nvCT. This study examined the nucleic acid amplification tests (NAATs) currently used (in 2009) for C. trachomatis detection in laboratories from 17 European countries and five countries/regions outside Europe that are participating in the United Kingdom (UK) National External Quality Assessment Service (NEQAS). It further examined changes in these laboratories' testing strategy during the period from 2006 to 2009, and their performance regarding nvCT detection. A UK NEQAS blinded nvCT specimen was distributed to all 283 participating laboratories, which were asked to analyse the specimen according to their routine C. trachomatis diagnostic protocols for endocervical swabs. BD ProbeTec was the most commonly used NAAT, followed by Cobas Amplicor, Cobas TaqMan, and Aptima. From 2006 to 2009, the use of Cobas Amplicor, which does not detect the nvCT, decreased, but it was still used by 22% (n=57) of responding participants in 59% of the countries, 54 of these 57 used it as first assay. Virtually all of the other participants detected the nvCT correctly. Laboratories using commercial or in house NAATs that do not detect the nvCT are encouraged to carefully monitor their C. trachomatis incidence, participate in effective internal and external quality assurance and controls schemes, and to consider changing their testing system. PMID:19442398

  4. H08117: NOS Hydrographic Survey , Roche Harbor and Mosquito Pass, Washington, 1959-04-28

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The National Oceanic and Atmospheric Administration (NOAA) has the statutory mandate to collect hydrographic data in support of nautical chart compilation for safe...

  5. Defending the Freedom to Innovate: Faculty Intellectual Property Rights after "Stanford v. Roche"

    Science.gov (United States)

    American Association of University Professors, 2014

    2014-01-01

    Tensions over faculty control of the fruits of their scholarship have been slowly building since the 1980s, but they have also intensified since late 2011. There have long been differences of opinion over ownership of patentable inventions, but over the last two years a number of universities have categorically asserted that they own these…

  6. H08116A: NOS Hydrographic Survey , Roche Harbor and Mosquito Pass, Washington, 1954-09-13

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The National Oceanic and Atmospheric Administration (NOAA) has the statutory mandate to collect hydrographic data in support of nautical chart compilation for safe...

  7. Analytical validation of the Roche 25-OH Vitamin D Total assay

    DEFF Research Database (Denmark)

    Knudsen, Cindy Soendersoe; Nexo, Ebba; Højskov, Carsten Schriver;

    2012-01-01

    evaluated the 25-OH Vitamin D Total assay in comparison to our in-house liquid chromatography tandem mass spectrometry (LC-MS/MS) method [194 patient samples without 25-hydroxy vitamin D2 (25OHD2) and 23 patient samples containing 25OHD2]. Results: At concentrations of 34 and 56 nmol/L within-run CVs were 4.......8% and 1.9% and total CVs were 8.3% and 6.1%. We verified that the limit of quantification was 22.5 nmol/L, as stated by the manufacturer. No significant difference was observed between serum and plasma samples (Li-heparin). Comparison with LC-MS/MS using 194 samples containing 25OHD3 only (no 25OHD2...

  8. Assessment of HPV infection among female university students in Honduras via Roche linear array

    NARCIS (Netherlands)

    Ferrera, A.; Tabora, N.; Flores, Y.; Zelaya, A.; Massuger, L.F.A.G.; Melchers, W.J.G.

    2011-01-01

    OBJECTIVE: To determine the prevalence of specific types of human papillomavirus (HPV), and the association with possible risk factors, among female university students at university in Honduras. METHODS: In a cross-sectional study, cervical samples from 400 women aged 18-35years were tested using a

  9. H08116: NOS Hydrographic Survey , Roche Harbor and Mosquito Pass, Washington, 1954-09-13

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The National Oceanic and Atmospheric Administration (NOAA) has the statutory mandate to collect hydrographic data in support of nautical chart compilation for safe...

  10. Cross-reactivity of amphetamine analogues with Roche Abuscreen radioimmunoassay reagents

    Energy Technology Data Exchange (ETDEWEB)

    Cody, J.T. (Air Force Drug Testing Laboratory, Brooks AFB, TX (USA))

    1990-01-01

    Cross-reactivity of amphetamine analogues with the Abuscreen amphetamine radioimmunoassay reagents was determined for both the standard and high specificity antibody systems. Compounds tested included 2-methoxyamphetamine, 4-hydroxymethamphetamine, 2,5-dimethoxyamphetamine (DMA), 4-bromo-2,5-dimethoxyamphetamine (DOB), 4-bromo-2,5-dimethoxy-beta-phenethylamine (BDMPEA), 3,4,5-trimethoxyamphetamine (TMA), 3,4-methylenedioxyamphetamine (MDA), N,N-dimethyl-3,4-methylenedioxyamphetamine and N-hydroxy-3,4-methylenedioxyamphetamine (N-OH MDA), 3,4-methylenedioxymethamphetamine (MDMA), 3,4-methylenedioxyethylamphetamine (MDEA), 2,5-dimethoxy-4-ethylamphetamine, 2,5-dimethoxy-4-methylamphetamine (DOM), and 3,4,5-trimethoxyphenethylamine (mescaline). Blank negative reference material was spiked with 1,000 to 100,000 ng/mL of the amphetamine analogue and used as sample in the assays. MDA was the only analogue that showed cross reactivity equal to or greater than that of amphetamine. None of the other analogue compounds demonstrated a positive result at even the highest concentration; however several showed depressed counts at various concentration levels.

  11. H08115: NOS Hydrographic Survey , Roche Harbor and Mosquito Pass, Washington, 1954-06-29

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The National Oceanic and Atmospheric Administration (NOAA) has the statutory mandate to collect hydrographic data in support of nautical chart compilation for safe...

  12. Roche genome sequencer FLX based high-throughput sequencing of ancient DNA

    DEFF Research Database (Denmark)

    Alquezar-Planas, David E; Fordyce, Sarah Louise

    2012-01-01

    Since the development of so-called "next generation" high-throughput sequencing in 2005, this technology has been applied to a variety of fields. Such applications include disease studies, evolutionary investigations, and ancient DNA. Each application requires a specialized protocol to ensure tha...

  13. The Swedish new variant of Chlamydia trachomatis (nvCT) remains undetected by many European laboratories as revealed in the recent PCR/NAT ring trial organised by INSTAND e.V., Germany.

    Science.gov (United States)

    Reischl, U; Straube, E; Unemo, M

    2009-01-01

    The May 2009 round of INSTAND's ring trial "Chlamydia trachomatis detection PCR/NAT" included a sample with high amount of the Swedish new variant of C. trachomatis (nvCT). A spectrum of at least 12 different commercial diagnostic nucleic acid amplification tests (NAATs) and many different in house NAATs were applied by the 128 participating laboratories which reported 152 results. Approximately 80% of the results correctly reported the presence of C. trachomatis in the nvCT specimen. The nvCT sample was mainly missed, as expected, by participants using the Roche COBAS Amplicor CT/NG (15.5% of reported results) but also by several participants using in house NAATs. The trend towards using nvCT-detecting NAATs is obvious and in addition to the new dual-target NAATs from Roche and Abbott, and BD ProbeTec ET, also a number of new CE mark-certified commercial tests from smaller diagnostic companies as well as many different in house NAATs were used. Laboratories using commercial or in house NAATs that do not detect the nvCT are encouraged to carefully monitor their C. trachomatis incidence, participate in appropriate external quality assurance and controls schemes, and consider altering their testing system. The reliable detection of low amounts of the wildtype C. trachomatis strain in other samples of the ring trial set indicates a good diagnostic performance of all applied commercial NAATs while also detecting the nvCT strain. PMID:19679035

  14. Analysis of heavy oils flows in saturated rocks; Analyse des ecoulements d'huiles lourdes dans les roches saturees

    Energy Technology Data Exchange (ETDEWEB)

    Luc Dormieux; Denis Garnier [Ecole Nationale des Ponts et Chaussees, 6 et 8 avenue Blaise Pascal Cite Descartes - Champs/Marne, 77455 Marne la Vallee Cedex 2, (France); Thierry Yalamas; Elisabeth Bemer; Jean-Francois Nauroy [Institut Francais du Petrole 1 et 4 avenue de Bois-Preau, 92852 Rueil-Malmaison Cedex, (France)

    2005-07-01

    In heavy oil reservoirs, the fluid is a viscous material, possibly nonlinear. In order to describe the flow of such oils in the rock, an extension of Darcy law to non Newtonian fluids is presented. Besides, oil production in this type of reservoir is responsible for rock erosion and the formation of a paste (slurry) made up of a mixture of oil and sand grains is observed. Based on a non linear homogenization technique, a modelling of the constitutive behavior of this slurry is proposed. (authors)

  15. Roche-lobe overflow systems powered by black holes in young star clusters: the importance of dynamical exchanges

    CERN Document Server

    Mapelli, Michela

    2014-01-01

    We have run 600 N-body simulations of intermediate-mass (~3500 Msun) young star clusters (SCs) with three different metallicities (Z=0.01, 0.1 and 1 Zsun). The simulations include the dependence of stellar properties and stellar winds on metallicity. Massive stellar black holes (MSBHs) with mass >25 Msun are allowed to form through direct collapse of very massive metal-poor stars (Z20 per cent of all RLO binaries are powered by MSBHs. The vast majority of RLO binaries powered by MSBHs are RLO-EBs. We have produced optical color-magnitude diagrams of the simulated RLO binaries, accounting for the emission of both the donor star and the irradiated accretion disk. We find that RLO-PBs are generally associated with bluer counterparts than RLO-EBs. We compare the simulated counterparts with the observed counterparts of nine ultraluminous X-ray sources. We discuss the possibility that IC 342 X-1, Ho IX X-1, NGC 1313 X-2 and NGC 5204 X-1 are powered by a MSBH.

  16. Migration de l’iode et du sélénium au travers de roches argileuses

    OpenAIRE

    Frasca, Benjamin

    2011-01-01

    Deep argillaceous formations are considered as potential host rock for high-level radioactive waste repository. Based on safety assessment calculations, active selenium (79Se) and iodine (129I) from high level radioactive waste might be ones of the major dose contributors due to their longevity and their anionic character. However, because of their high sensitivity to redox condition, a special attention to the oxidation state of these elements is required. A comparative study on the diffusio...

  17. Analytical performance of the Roche LightCycler® Mycobacterium Detection Kit for the diagnosis of clinically important mycobacterial species.

    Directory of Open Access Journals (Sweden)

    Shaheed V Omar

    Full Text Available BACKGROUND: The LightCycler® Mycobacterium Detection Kit based on real-time PCR technology for the detection of Mycobacterium tuberculosis, Mycobacterium avium and Mycobacterium kansasii was recently developed. This study evaluated its analytical sensitivity, specificity and reproducibility. METHODOLOGY/PRINCIPAL FINDINGS: Plasmid standards were prepared and used to determine the limit of detection. The assay was also performed against organisms other than mycobacteria, other mycobacterial strains and interfering substances to exclude cross-reactivity and interference. Reference standards were prepared and tested to assess the assay's reproducibility. All PCR assays were performed using the LightCycler® 2.0 Instrument. The detection limit for M. tuberculosis was 28 copies per microlitre. Neither cross-reactivity nor interference occurred with non-mycobacterial organisms and substances tested. Overall reproducibility for consecutive measurements, run-to-run, lot-to-lot, day-to-day and laboratory-to-laboratory achieved a coefficient of variance of less than two percent. SIGNIFICANCE: The LightCycler® Mycobacterium Detection kit has shown to be a robust and accurate assay with the potential to be used as a rapid TB diagnostic test.

  18. Near-complete genome sequencing of swine vesicular disease virus using the Roche GS FLX sequencing platform

    DEFF Research Database (Denmark)

    Nielsen, Sandra Cathrine Abel; Bruhn, Christian Anders Wathne; Samaniego Castruita, Jose Alfredo;

    2014-01-01

    that is suitable for sequencing the complete protein-encoding sequences of SVDV isolates in which the RNA is relatively intact. The approach couples a single PCR amplification reaction, using only a single PCR primer set to amplify the near-complete SVDV genome, with deep-sequencing using a small fraction...

  19. HPV: screening and prevalence of genotypes in the lower-Molise

    Directory of Open Access Journals (Sweden)

    Mariangela Spinosa

    2010-06-01

    Full Text Available HPV is a double-stranded DNA virus. It is a sexually transmitted virus about 75% of women contact it throughout their lives.Among the 100 classified genotypes about 40 can infect the genital mucosa. Depending on the type of lesion can be identified genotypes with a “low risk” and “high risk”.These are associated with cervical dysplasia and carcinoma of the uterine cervix. Il “Pap smear” is the cytological test that highlights the changes of the cervical cells. L’’HPV-DNA-PCR can detect DNA and determine the infecting viral genotype. It is a susceptible, specific and not invasive. test.Aim of our work was to evaluate a screening program aimed at testing the prevalence of HPV genotypes in the lower-Molise. We have examined 339 samples obtained from cervical swabs of women aged 18-45 years.Was used-Amplicor HPV test (Roche a qualitative method for detection of 13 high-risk genotypes. Genotype was identified using the Linear Array HPV-Genotyping (Roche method. Among 339 women tested, 292 (86% were negative, 47 (14% positive. It has been performed genotyping on 47 positiv samples. 16 and 18 were the prevalent genotypes (average 8%.There was a lower prevalence between 3% and 2% among the other genotypes. These results allow us to draw some considerations while taking into account the limited number of samples.The frequency of positive HPVDNA test it is very high.The HPV-DNA testing is a valuable aid in diagnosis by HPV alongside the Pap Test. The prevalence of genotypes found in the Low-Molise is consistent with data reported in literature.The genotypes 16 and 18 have a higher frequency, taking into account that these genotypes are responsible for 70% of cervical cancer, the determination may be a useful aid in the diagnosis and prevention.

  20. Sieroprevalenza di infezione da HBV e HCV tra pazienti in dialisi

    Directory of Open Access Journals (Sweden)

    Rosa Anna Leone

    2003-12-01

    Full Text Available The aim of the present study was to investigate the seroprevalence of HBV and HCV among dialysis patients in the Lamezia Terme (CZ area during the period 1999-2002. Sera from 63 patients in haemodialysis (HD and 10 patients in peritoneal dialysis (PD were analyzed with a follow-up every three months for HBsAg, HBcAb, HBsAb, anti-HCV and anti-HIV (Elisa Test,AxSYM,Abbott;we analyzed reactive sera for anti-HCV by using supplemental test (RIBA Test, Ortho; we also looked for viremia (RT-PCR Amplicor, Roche Diagnostics and HCV genotypes (Inno-Lipa HCV II, Innogenetics.The results show that, among the HD patients, 3 were HBsAg positive (Chronic Infection and 7 HBcAb and HBsAb positive/HBsAg negative (Passed Infection; 14 individuals were anti-HCV positive. No patients in PD were positive for HBV and HCV markers.The prevalence of chronic HBV infection was 4.8% (instead of 3% in other Dialysis Units, that of anti-HCV positive was 22% (in others 24%- 33%; among anti-HCV positive patients, the HCV-RNA prevalence was 79% (instead of 80%; the most recurrent HCV genotype was 2a/2c (instead of 1b in general population.These findings lead us to hypothesize that the environmental transmission in the dialysis setting is tightly correlated to the risk of HBV and HCV infection.

  1. Single-step PCR in molecular diagnosis of hepatitis C virus infection.

    Science.gov (United States)

    Farma, E; Boeri, E; Bettini, P; Repetto, C M; McDermott, J; Lillo, F B; Varnier, O E

    1996-12-01

    The diagnostic utility of two PCR systems and three PCR detection methods for hepatitis C virus (HCV) RNA was evaluated in serum samples. A nested PCR was considered the reference assay and was compared with two single-step PCR methods: the first is based on the detection of PCR products by liquid hybridization with a 32P-end-labeled probe, and the second is the Roche Amplicor colorimetric assay using microwell plate hybridization with a specific nucleic acid probe. Using the Pelicheck HCV RNA Eurohep genotype 1 proficiency panel, our laboratory achieved medium-high levels of performance with all three methods. The highest sensitivity was, however, observed with the isotopic single-step PCR (ss-PCR) method. The analytical sensitivity of ss-PCR with isotopic detection and ss-PCR with colorimetric detection was identical to that of nested PCR, with a 100% result concordance. Comparison of ss-PCR with enzyme-linked immunosorbent and RIBA assays in the analysis of clinical samples showed a high concordance. ss-PCR methods appear more suitable for diagnostic application. Nevertheless, HCV RNA PCR cannot be considered a screening assay; it should be requested in the presence of reactive serology or specific clinical symptomatology with altered liver parameters, and it is a potential tool for the follow-up of patients with HCV infection.

  2. Stable hepatitis C virus RNA detection by RT-PCR during four days storage

    Directory of Open Access Journals (Sweden)

    Horsmans Yves

    2002-10-01

    Full Text Available Abstract Background Suboptimal specimen processing and storage conditions of samples which contain hepatitis C virus (HCV RNA may result in a decline of HCV RNA concentration or false-negative results in the detection of HCV RNA in serum. We evaluated the stability of HCV RNA in serum and clotted blood samples stored at room temperature or at 4°C for 4 days with the aim of optimizing the standard procedures of processing and storage of samples. Methods Blood from five HCV RNA positive patients was collected in tubes with and without separator gel, centrifuged 1 or 6 hours after collection. Samples were then left 6, 24, 48, 72 or 96 h at room temperature (21.5 – 25.4°C or at 4°C before determining their HCV RNA level using the COBAS AMPLICOR HCV MONITOR Test, vs 2.0 (Roche Diagnostic Systems. Results The logarithm of the HCV RNA level measurements remained within a 0.3 value of the means for 4 days at both temperatures (room temperature or 4°C. Conclusions We conclude that blood samples may be collected and aliquoted within 6 h of collection and can be stored at 4°C for 72 hours as proposed by the manufacturer without significant differences in measured HCV RNA level. Our results indicate that lapses in this scheme may still yield reliable results.

  3. Persistence of hepatitis C virus in a white population: associations with human leukocyte antigen class 1.

    LENUS (Irish Health Repository)

    Fanning, Liam J

    2012-02-03

    The aim of this study was to define novel associations between human leukocyte antigen (HLA) class 1 alleles and persistence or clearance of the hepatitis C virus (HCV) in a white population. All individuals in the study were seropositive for anti-HCV antibodies. Viral status was determined by the Roche HCV Amplicor test. HLA-A, -B, -C allelic group profile was molecularly defined by reverse line probe hybridization. The strongest individual allelic group associations with persistent HCV infection were HLA A*11 (p = 0.044) and Cw*04 (p = 0.006). However, only the HLA C*04 association survived correction for multiple comparisons. Further analysis of alleles in linkage with HLA Cw*04 revealed that the haplotype HLA A*11, Cw*04 was present in 11 individuals, 10 of whom were viremic (p = 0.05). No gene dosage effect was observed. No association between HLA class 1 allelic groups and aviremia and virus load was evident in this white population. HLA B*44 is associated with low virus load in human immunodeficiency virus disease, but this association was not evident in this HCV-infected population. Novel HLA class 1 alleles associated with persistence of HCV have been identified.

  4. Lyophilized standards for the calibration of real time PCR assay for hepatitis C virus RNA

    Institute of Scientific and Technical Information of China (English)

    WANG Lu-nan; WU Jian-min; DENG Wei; SHEN Zi-yu; CHEN Wen-xiang; LI Jin-ming

    2006-01-01

    Background Since October 1997, an international standard for hepatitis C virus (HCV) nucleic acid amplification technology assay, 96/790, has been available. We compared a series of lyophilized standards with known HCV RNA concentrations against the international standard in fluorescence quantitative PCR detection.Methods A series of lyophilized sera were calibrated by ROCHE COBAS AMPLICOR HCV Monitor test against the international standard and sent to various manufacturers to analyse the samples using their own kits.Then calibration curves from the series were compared with that obtained from the external standard calibration curve with the manufacture's series.Results The standard calibration curve with the series of lyophilized serum showed an excellent correlation(R2>0.98), slope and intercept that were similar to those from the manufacture's series. When the standard calibration curve from the series of lyophilized standards were used to define the values of the given sample,lower coefficients of variation between kits from different manufactures were obtained.Conclusion The results showed that the lyophilized standards could be used to setup the standard calibration curve for clinical HCV RNA quantitative PCR detection.

  5. Single genome amplification of proviral HIV-1 DNA from dried blood spot specimens collected during early infant screening programs in Lusaka, Zambia.

    Science.gov (United States)

    Seu, Lillian; Mwape, Innocent; Guffey, M Bradford

    2014-07-01

    The ability to evaluate individual HIV-1 virions from the quasispecies of vertically infected infants was evaluated in a field setting at the Centre for Infectious Disease Research in Zambia. Infant heel-prick blood specimens were spotted onto dried blood spot (DBS) filter paper cards at government health clinics. Nucleic acid was extracted and used as a template for HIV-1 proviral DNA detection by a commercial Amplicor HIV-1 PCR test (Roche, version 1.5). On samples that tested positive by commercial diagnostic assay, amplification of DNA was performed using an in-house assay of the 5' and 3' region of the HIV-1 genome. Additionally, fragments covering 1200 nucleotides within pol (full length protease and partial reverse transcriptase) and 1400 nucleotides within env (variable 1-variable 5 region) were further analyzed by single genome amplification (SGA). In summary, we have demonstrated an in-house assay for amplifying the 5' and 3' proviral HIV-1 DNA as well as pol and env proviral DNA fragments from DBS cards collected and analyzed entirely in Zambia. In conclusion, this study shows the feasibility of utilizing DBS cards to amplify the whole proviral HIV-1 genome as well as perform SGA on key HIV-1 genes.

  6. Diagnosis of Neisseria gonorrhoeae Using Molecular Beacon

    Directory of Open Access Journals (Sweden)

    Divya Sachdev

    2015-01-01

    Full Text Available Neisseria gonorrhoeae is an important sexually transmitted diseases (STD causing pathogen worldwide. Due to absence of an affordable diagnostic assay, routine screening of gonococcal infection becomes impossible in developing countries where infection rates are maximum. Treatment is given on the basis of symptoms alone which leads to spread of infection. Thus, development of a rapid, sensitive, specific, and PCR based visual diagnostic assay suitable for developing countries, required for better disease management, is aimed at in present study. Endocervical swabs were collected from patients visiting gynecology department of various hospitals in Delhi. In-house PCR based assay was developed and modified to visual assay using molecular beacon for end-point detection. It was evaluated against Roche AMPLICOR NG kit and rmp gene. Specificity of beacon was confirmed by competition experiments. Diagnostic test was 98.21% specific and 99.59% sensitive whereas negative and positive predicted value were 99.40% and 98.78%, respectively. We also observed that twice the concentration (2X of premix was stable at 4°C for 4 months and dry swab samples gave concordant results with that of wet swabs. These features make the test best suitable for routine diagnosis of genital infections in developing countries.

  7. Does chronic alcohol use by HIV-infected patients on d4T/3TC/NVP drug regimen effect the HIV viral load and what is the therapeutic window of the drugs, CD4+ count and WBC count in patients with high viral load during the 9 months period of follow up?

    Directory of Open Access Journals (Sweden)

    Godfrey S. Bbosa

    2013-10-01

    Full Text Available The study investigated the effects of chronic alcohol use on HIV viral load in HIV-infected patients on d4T/3TC/NVP drug regimen during 9 months follow up period. It also determined plasma drug concentrations of d4T, 3TC and NVP; CD4+ and WBC counts for patients with high HIV viral load. A case-control study using repeated measures with serial measurements was used. A total of 41 patients (20 alcohol group and 21 control group were screened for alcohol use using WHO AUDIT tool and chronic alcohol use biomarkers. Blood sampling was done at 3 month intervals for a period of 9 months. HIV viral load was determined using Roche Amplicor HIV-1 monitor test, version 1.5 (Amplicor. The d4T, 3TC and NVP concentrations were determined by Shimadzu Class-VPTM HPLC Chromatography data system version 6.1. The CD4+ cell count was determined using FACSCalibur flow cytometer. The WBC was determined using automated hematological Coulter CBC-5 Hematology Analyzer system. Results show that % patients with HIV viral load ≥400 copies/ml in control group was highest (23.8%, n=5 at 3 month while in chronic alcohol use group, it was at 0 month (35%, n=7 for both WHO AUDIT tool and chronic alcohol-use biomarkers groups. Generally patients with high viral load ≥400 copies/ml was observed in chronic alcohol use as compared to control group in both WHO AUDIT tool and biomarkers group despite of patients having high steady state d4T, 3TC and NVP plasma drug concentrations in circulation that is available to suppress HIV virus. The high viral load could be associated with the emergence of resistance of the HIV virus and these patients generally had a low CD4+ cell count. Some of these patients had no detectable d4T plasma drug concentrations in circulation and most of them with high viral load had sub-therapeutic NVP plasma drug concentrations in their blood circulation. Chronic ethanol use by HIV-infected patients on d4T/3TC/NVP drug regimen increased HIV viral load and

  8. Nucleic acid amplification technology screening for hepatitis C virus and human immunodeficiency virus for blood donations

    International Nuclear Information System (INIS)

    To investigate the performance of the commercial Roche COBAS AmpliScreen assay, and demonstrate whether the COBAS AmpliScreen human immunodeficiency virus-1 (HIV-1) test, v1.5, and COBAS AmpliScreen hepatitis C virus (HCV) v 2.0 for screening for HIV-1 and HCV RNA in the donated blood units from which plasma mini pools were collected, by nucleic acid amplification technology (NAT), could detect the positive pools and reduce the risk of transmission of infections for those routinely tested by serological assays. The study was performed on 3288 plasma samples collected from blood donors in a period of 13 months, from August 2004 to August 2005, at Al-Hada Armed Forces Hospital, Molecular Pathology Laboratory, Taif, Kingdom of Saudi Arabia. The samples were tested by the reverse transcriptase polymerase chain reaction (RT-PCR) after RNA extraction (this represents the major method in NAT assays), in parallel with the routine serological testing to detect qualitatively for HIV-1 and HCV. The NAT assays that include an automated COBAS AmpliPrep system for RNA extraction and COBAS Amplicor Analyzer using AmpliScreen kits for RT-PCR assays, and the routine serological screening assays for the detection of the HIV-1 and HCV RNA in the plasma samples from the blood donors have shown to be a reliable combination that would meet our requirements. The collected data further confirms the results from the serological assays and enables us to decrease the residual risk of transmission to a minimum with the finding of no seronegative window period donation. The results demonstrate that out of 3288 samples, the percentages of RT-PCR (NAT) negative blood donations that were also confirmed as seronegative were 99% for HCV, and 99.1% for HIV-1. The modified combined systems (automated COBAS AmpliPrep system for RNA extraction and COBAS Amplicor Analyzer using AmpliScreen kits for RT-PCR assays) for NAT screening assays has allowed the release of all blood donations supplied in the

  9. Treatment seeking behaviors related to gonorrhea among female sex workers in 7 cities in Indonesia

    Directory of Open Access Journals (Sweden)

    Roselinda Roselinda

    2014-02-01

    Full Text Available Abstrak Latar belakang:Gonore merupakan salah satu infeksi menular seksual yang menjadi permasalahan besar kesehatan terutama pada wanita penjaja seks (WPS di Indonesia. Tujuan dari artikel ini adalah untuk melihat hubungan antara pola pencarian pengobatan gonore. Metode:Data berasal dari studi potong lintang dengan responden WPS yang dipilih secara cluster random sampling dari 7 kota (Timika, Yogyakarta, Kupang, Samarinda, Pontianak, Makassar dan Tangerang di Indonesia pada tahun 2007. Diagnosis gonore berdasarkan hasil pemeriksaan Polymerase Chain Reaction (PCR menggunakan Amplicor CT/NG dari Roche yang telah disetujui oleh World Health Orgazation (WHO sebagai alat skrining gonore. Hasil:Proporsi responden yang menderita gonore sebesar 26.1% (404/1750. Persentase penderita gonore yang melakukan upaya pengobatan terdistribusi hampir sama dengan yang mengunjungi fasilitas kesehatan / dokter dengan yang membeli obat sendiri. Subyek yeng melakukan pengobatan tradisional memiliki risiko 44% lebih tinggi menderita gonore dibandingkan dengan subyek yang melakukan pengobatan di fasilitas kesehatan / dokter [risiko relatif suaian (RRa = 1,44; P = 0.044]. Sedangkan subyek yang tidak diobati dibandingkan dengan yang berobat ke fasilitas kesehatan / dokter lebih berisiko 55% menderita gonore (RRa = 1.55; P = 0.002.Kesimpulan: Wanita penjaja seks yang melakukan maupun yang tidak pengobatan tradisional dibandingkan dengan yang mengunjungi fasilitas kesehatan/dokter memiliki risiko yang lebih tinggi menderita gonore. (Health Science Indones 2013;2:87-92Kata kunci:gonore, wanita penjaja seks, IndonesiaAbstractBackground:Gonorrhea is one of sexually transmitted infections that have become a major health problem especially among female sex workers (FSW in Indonesia. The objective of this article is to identify the relationship between treatment seeking behaviors, the sites of study and gonorrhea among FSW. Methods: The data that analyzed derived from cross

  10. Variables that influence HIV-1 cerebrospinal fluid viral load in cryptococcal meningitis: a linear regression analysis

    Directory of Open Access Journals (Sweden)

    Cecchini Diego M

    2009-11-01

    Full Text Available Abstract Background The central nervous system is considered a sanctuary site for HIV-1 replication. Variables associated with HIV cerebrospinal fluid (CSF viral load in the context of opportunistic CNS infections are poorly understood. Our objective was to evaluate the relation between: (1 CSF HIV-1 viral load and CSF cytological and biochemical characteristics (leukocyte count, protein concentration, cryptococcal antigen titer; (2 CSF HIV-1 viral load and HIV-1 plasma viral load; and (3 CSF leukocyte count and the peripheral blood CD4+ T lymphocyte count. Methods Our approach was to use a prospective collection and analysis of pre-treatment, paired CSF and plasma samples from antiretroviral-naive HIV-positive patients with cryptococcal meningitis and assisted at the Francisco J Muñiz Hospital, Buenos Aires, Argentina (period: 2004 to 2006. We measured HIV CSF and plasma levels by polymerase chain reaction using the Cobas Amplicor HIV-1 Monitor Test version 1.5 (Roche. Data were processed with Statistix 7.0 software (linear regression analysis. Results Samples from 34 patients were analyzed. CSF leukocyte count showed statistically significant correlation with CSF HIV-1 viral load (r = 0.4, 95% CI = 0.13-0.63, p = 0.01. No correlation was found with the plasma viral load, CSF protein concentration and cryptococcal antigen titer. A positive correlation was found between peripheral blood CD4+ T lymphocyte count and the CSF leukocyte count (r = 0.44, 95% CI = 0.125-0.674, p = 0.0123. Conclusion Our study suggests that CSF leukocyte count influences CSF HIV-1 viral load in patients with meningitis caused by Cryptococcus neoformans.

  11. Chlamydia trachomatis infection and sexual behaviour among female students attending higher education in the Republic of Ireland.

    LENUS (Irish Health Repository)

    O'Connell, Emer

    2009-10-29

    BACKGROUND: There are no prevalence data on Chlamydia trachomatis relating to female students attending higher education available for the Republic of Ireland. This information is required to guide on the necessity for Chlamydia screening programmes in higher education settings. This research aimed to determine the prevalence of and predictive risk factors for Chlamydia trachomatis genital infection among female higher education students in Ireland. METHODS: All females presenting during one-day periods at Student Health Units in three higher education institutions in two cities in the Republic of Ireland were invited to participate. Participants completed a questionnaire on lifestyle and socio-demographic factors and provided a urine sample. Samples were tested for C. trachomatis DNA by a PCR based technique (Cobas Amplicor, Roche). To examine possible associations between a positive test and demographic and lifestyle risk factors, a univariate analysis was performed. All associations with a p value < 0.05 were included in a multivariate logistic regression analysis. RESULTS: Of the 460 sexually active participants 22 tested positive (prevalence 4.8%; 95% CI 3.0 to 7.1%). Variables associated with significantly increased risk were current suggestive symptoms, two or more one-night stands and three or more lifetime sexual partners. The students displayed high-risk sexual behaviour. CONCLUSION: The prevalence of C. trachomatis infection and the lack of awareness of the significance of suggestive symptoms among sexually experienced female students demonstrate the need for a programme to test asymptomatic or non-presenting higher education students. The risk factors identified by multivariate analysis may be useful in identifying those who are most likely to benefit from screening. Alcohol abuse, condom use, sexual behaviour (at home and abroad) and, knowledge of sexually transmitted infections (STIs) (including asymptomatic nature or relevant symptoms) were

  12. Indeterminate RIBA results were associated with the absence of hepatitis C virus RNA (HCV-RNA in blood donors

    Directory of Open Access Journals (Sweden)

    Felicidade Mota Pereira

    2014-01-01

    Full Text Available Introduction: Hepatitis C virus (HCV infection is diagnosed by the presence of antibodies and is supplemented by confirmatory testing methods, such as recombinant immunoblot assay (RIBA and HCV-RNA detection. This study aimed to evaluate the efficacy of RIBA testing to diagnose HCV infection in blood donors positive for anti-HCV antibodies. Methods: A total of 102 subjects positive for anti-HCV determined by enzyme-linked immunosorbent assay (ELISA at the Hematology and Hemotherapy Foundation of Bahia (HEMOBA were later assessed with new samples using the Abbott Architect anti-HCV test (Abbott Diagnostics, Wiesbaden, Germany, the RIBA III test (Chiron RIBA HCV 3.0 SIA, Chiron Corp., Emeryville, CA, USA, the polymerase chain reaction (PCR; COBAS® AMPLICOR HCV Roche Diagnostics Corp., Indianapolis, IN, USA and line probe assay (LiPA - Siemens, Tarrytown, NY, USA genotyping for HCV diagnosis. Results: Of these new samples, 38.2% (39/102 were positive, 57.8% (59/102 were negative and 3.9% (4/102 were indeterminate for anti-HCV; HCV-RNA was detected in 22.5% (23/102 of the samples. RIBA results were positive in 58.1% (25/43, negative in 9.3% (4/43 and indeterminate in 32.6% (14/43 of the samples. The prevailing genotypes were 1 (78.3%, 18/23, 3 (17.4%, 4/23 and 2 (4.3%, 1/23. All 14 samples with indeterminate RIBA results had undetectable viral loads (detection limit ≤50 IU/mL. Of these samples, 71.4% (10/14 were reevaluated six months later. Eighty percent (8/10 of these samples remained indeterminate by RIBA, and 20% (2/10 were negative. Conclusions: In this study, individuals with indeterminate RIBA results had no detectable HCV-RNA.

  13. Cicatrization of the damaged zone around a tunnel in low permeable rocks; Cicatrisation de la zone endommagee autour du tunnel dans des roches peu permeables

    Energy Technology Data Exchange (ETDEWEB)

    Hamza, R.O

    2005-12-15

    Three types of clays have been studied in this work: the Mol clay (plastic clay), the Mont Terri clay (opalinus clay) and the East clay (stiff clay). This work is divided into three parts: an experimental part which has allowed to identify the cicatrization phenomenon and the cracks closing. Through several tests has been determined the evolution of the permeability of the clayey samples which are undamaged or damaged with time and according to the different applied hydro-mechanical loadings. In order to describe the cicatrization phenomenon, a rheological law has been developed from the experimental observations and under some hypotheses. The parameters of the cicatrization model have been determined by numerical simulation. In order to take into account the structures effects at a large scale, the modelling of a tunnel has allowed to better identify the cicatrization phenomenon and to analyze the long term stability of this work. (O.M.)

  14. [Results transferability on RXL, ARX, X-Pand, BN2 (Dade Behring) and modular DP (Roche Diagnostics) analysers: application to component assays of fibrotest and Actitest].

    Science.gov (United States)

    Imbert-Bismut, F; Messous, D; Raoult, A; Poynard, T; Bertrand, J J; Marie, P A; Louis, V; Audy, C; Thouy, J M; Hainque, B; Piton, A

    2005-01-01

    The follow up of patients with chronic liver diseases and the data from multicentric clinical studies are affected by the variability of assay results for the same parameter between the different laboratories. Today, the main objective in clinical chemistry throughout the world is to harmonise the assay results between the laboratories after the confirmation of their traceability, in relation to defined reference systems. In this context, the purpose of our study was to verify the homogeneity of haptoglobin, apolipoprotein A1, total bilirubin, GGT activity, ALAT activity results, which are combined in Fibrotest and Actitest, between Dimension Analysers RXL, ARX and X-PAND (Dade Behring Society). Moreover, we verified the transferability of Fibrotest and Actitest results between the RXL, and either the BN2 (haptoglobin and apolipoprotein A1) or the Modular DP (total bilirubin, GGT and ALAT activity concentrations). The serum samples from 150 hospitalised patients were analysed on the different analysers. Specific protein assays were calibrated using solutions standardised against reference material on Dimension and BN2 analysers. Total bilirubin assays were performed by a diazoreaction on Dimension and Modular DP analysers. The GGT and ALAT activity measurements on the Dimension analysers were performed in accordance with the reference methods defined by the International Federation of Clinical Chemisty and Laboratory Medicine (IFCC). On the Modular, enzyme activity measurements were performed according to the Szasz method (L-gamma- glutamyl-4-nitroanilide as substrate) modified by Persijn and van der Slik (L-gamma- glutamyl-3-carboxy- 4-nitroanilide as substrat) for GGT and according to the IFCC specifications for ALAT. The methods of enzymatic activity measurement were calibrated on the Modular only. Liver fibrosis and necroinflammatory activity indices were determined using calculation algorithms, after having adjusted each component's result of Fibrotest and Actitest for gender and age. Our study has shown, for each parameter, harmonious results between the Dimension analysers and between RXL and BN2- Modular DP. Disregarding alpha-2 macroglobulin which cannot be assayed on RXL, the results of Fibrotest and Actitest were similar as performed on BN2- Modular DP and RXL. PMID:15951262

  15. Natural fracturing of rocks: application to the Ahnet basin (Algeria); Fracturation naturelle des roches: application au bassin de l`Ahnet (Algerie)

    Energy Technology Data Exchange (ETDEWEB)

    Badsi, M.

    1998-07-06

    In the Ahnet basin, the production seems to be unrelated to lithological variations in the reservoirs. In these large anticline structures, located in the central Ahnet basin, the presence of gas has been proven, but only a few production wells have been moderately successful. This inconsistency is probably related to the spatial distribution of fracturing throughout the reservoir. In order to investigate several hypothesis, we used several approach to solve problems posed by the interpreter: namely understanding the deformation process, predicting the fractured zones and building the discrete model of fracture network. This approach combines several methods, including sand box modelling, numerical modelling and Statistics rules, often related with fractal behaviour of faults families, have been used for extrapolating observations from seismic or from wells. The numerical tools and sand box analysis have allowed us to answer to the questions related to the formation of this large anticlines in the Ahnet basin and suggest a probable origin of the variation in the spatial distribution of natural fractures. The deterministic predictions of small-scale faults use probabilistic approaches for spatial interpolation assuming implicitly relationship between detected large faults and unresolved small faults. The statistical modelling is used to carry out analysis of the spatial variation of mean fracture attributes at the global scale (a few kilometers) and a 3D stochastic modelling of the fracture system at the local scale (a few ten of meters). (author) 139 refs.

  16. Stratigraphic and technological evidence from the middle palaeolithic-Châtelperronian-Aurignacian record at the Bordes-Fitte rockshelter (Roches d’Abilly site, Central France)

    DEFF Research Database (Denmark)

    Aubry, Thierry; Dimuccio, Luca Antonio; Almeida, Miguel;

    2012-01-01

    luminescence age of 39 ± 2 ka and feldspar infra-red stimulated luminescence age of 45 ± 2 ka of the sediments. The Bordes-Fitte rockshelter sequence represents an important contribution to the debate about the characterization and timing of the Châtelperronian, as well as its affinities to earlier and later...

  17. Damage-induced permeability changes around underground excavations; Endommagement des roches argileuses et permeabilite induite au voisinage d'ouvrages souterrains

    Energy Technology Data Exchange (ETDEWEB)

    Coll, C

    2005-07-15

    The storage of nuclear waste in deep geological formations is now considered more and more as a potential solution. During excavation, a disturbed zone develops in which damaging can be important and which can lead eventually to the failure of the rock. Fluid flow and permeability in the rock mass can be significantly modified producing a possible security risk. Our work consisted in an experimental study of the hydro-mechanical coupling of two argillaceous rocks: Boom clay (Mol, Belgium) and Opalinus clay (Mont-Terri, Switzerland). Triaxial tests were performed in a saturated state to study the permeability evolution of both clays with isotropic and deviatoric stresses. Argillaceous rocks are geo-materials with complex behaviour governed by numerous coupled processes. Strong physico-chemical interactions between the fluid and the solid particles and their very low permeability required the modification of the experimental set up. Moreover, specific procedures were developed to measure permeability and to detect strain localisation in shear bands. We show that for Boom Clay, permeability is not significantly influenced by strain localisation. For Opalinus clay, fracturing can induce an increase of the permeability at low confining pressure. (author)

  18. Approach to underground characterization of a disposal vault in granite; Methode de caracterisation souterraine d`une enceinte de stockage dans la roche granitique

    Energy Technology Data Exchange (ETDEWEB)

    Everitt, R.A.; Martin, C.D.; Thompson, P.M.

    1994-12-01

    The concept of disposing of nuclear fuel waste by sealing it in a disposal vault in the Canadian Shield is being investigated as part of the Canadian Nuclear Fuel Waste Management Program. Engineered and natural barriers would isolate the waste from the biosphere. Underground characterization and testing have been under way since 1983 at the Underground Research Laboratory in support of this program. This report draws on experience gained at the URL to recommend an approach to underground characterization to obtain information to optimize the design of the excavation and the engineered barriers, and to provide a baseline against which to monitor the performance of the facility during and following its operation.

  19. Reduction of Polymer Adsorption on Reservoir Rocks Réduction de l'adsorption des polymères sur les roches réservoirs

    Directory of Open Access Journals (Sweden)

    Chauveteau G.

    2006-11-01

    Full Text Available The adsorption properties of polyacrylamides and xanthans on mineral surfaces carrying silanol and aluminol groups such as sand and kaolinite are described. The influence of the main parameters such as the nature of adsorption sites, surface charge, chemical structure and conformation of polymer and interactions of mono- and divalent ions with polymer and mineral surface has been investigated and interpreted. Some operating parameters in polymer flooding such as pH and salinity of injected solution, the nature of the polymer and its degree of ionicity were found to be determining factors from the adsorption level. The results give key elements for reducing adsorption by a proper choice of polymer nature and ionicity and of injection conditions. Les propriétés d'adsorption des polyacrylamides et des xanthanes sur des surfaces minérales portant des groupements silanols et aluminols comme le sable et la kaolinite ont été examinées. L'influence de différents paramètres est analysée : nature des sites d'adsorption, charge de surface, structure chimique et conformation du polymère, interactions des ions mono et divalents avec la surface. Il apparaît que le pH et la salinité de la solution injectée, la nature du polymère et, en particulier son degré d'ionicité, qui sont les paramètres opérationnels lors d'une injection de polymère destinée à augmenter le taux de récupération du pétrole, sont déterminants en ce qui concerne les niveaux d'adsorption. On en déduit les principaux moyens pour réduire l'adsorption dans un cas d'application donné.

  20. Influence aqueous solutions on the mechanical behavior of argillaceous rocks; Influence des solutions aqueuses sur le comportement mecanique des roches argileuses

    Energy Technology Data Exchange (ETDEWEB)

    Wakim, J

    2005-12-15

    The hydration of the shale with an aqueous solution induces a swelling deformation which plays an important role in the behaviour of the structures excavated in this type of grounds. This deformation is marked by a three-dimensional and anisotropic character and involves several mechanisms like adsorption, osmosis or capillarity. Several researches were dedicated to swelling and were often much debated due to the complexity of the implied phenomena. The goal of this thesis is therefore to contribute to a better understanding of shale swelling when the rock is confined and hydrated with an aqueous solution. The main part of the work accomplished was related to the Lorraine shale and to the Tournemire shale. To characterize swelling and to identify the main governing parameters, it was necessary to start the issue with an experimental approach. Many apparatus were then developed to carry out tests under various conditions of swelling. In order to facilitate the interpretation of the tests and thereafter the modelling of the behaviour, the experimental procedure adopted consisted of studying first the mechanical aspect and then the chemical aspect of swelling. In the mechanical part, swelling was studied by imposing on the sample a mechanical loading while maintaining during the tests the same aqueous solution. The principal parameters which were studied are the effect of the lateral conditions on axial swelling (impeded strain or constant stress) as well as the influence of the axial stress on radial swelling. The anisotropy of swelling was studied by carrying out, for different orientations of the sample, tests of free swelling, impeded swelling and uniaxial swelling. These various mechanical tests allowed to study the three-dimensional anisotropic swelling in all the conditions and to select the most appropriate test to be used in the second phase of the research. The precise analysis performed to explain the mechanisms behind the swelling of an argillaceous rock when it is in contact of water showed that the mechanical response is ruled mainly by electrochemical phenomena which operate between the solution elements and the clay layers. This important result oriented the research to develop better the role of the chemistry in the behaviour. Swelling was therefore analysed by imposing on the sample a chemical loading while maintaining constant the axial stress. Monovalent and divalent salts which were used with various concentrations are the sodium chloride (NaCl), the potassium chloride (KCl) and the calcium chloride (CaCl{sub 2}). The foreseen objective was to analyze the effect of the chemical path with decreasing or cyclic concentrations and to highlight the role of the solution normality, the type of salt and the anisotropy on the axial swelling. The experimental results obtained allowed the development of a new rheological model which takes into account the time, the anisotropy, the applied loading and the chemistry of the solution. This model was implemented in a finite element code to simulate simple cases and to prove its validity. (author)

  1. About the identification of behaviour law parameters of clayey rocks; Sur l'identification des parametres des lois de comportement des roches argileuses

    Energy Technology Data Exchange (ETDEWEB)

    Lecampion, B

    2002-09-15

    This work aims at developing identification methods for clayey rock parameters. These methods are necessary for the interpretation of the numerous data obtained at the ANDRA's Meuse/Haute-Marne underground laboratory. Two main rheological aspects have been considered: the poro-elastic behaviour and the elasto-visco-plastic behaviour. The first part of the study focusses on the poro-elastic parameters. Chapter 2 recalls the direct problem and discusses some important points of the identification inverse problem. Chapter 3 deals with the formulation of gradient calculation techniques for the linear poro-elastic case. The resolution using the finite-element method is discussed. The direct and associated state differentiation methods are validated for a 2D numerical example using the finite-element code Cast3M. The identification of poro-elastic coefficients of the Meuse/Haute-Marne argillaceous rocks is discussed in detail in chapter 4. The use of approximate semi-explicit solutions of the direct problems allows to obtain a fast identification method. The second part deals with the identification of elasto-visco-plastic parameters. The visco-plastic behaviour of Meuse/Haute-Marne rocks is discussed in chapter 5 and a visco-plastic model with nonlinear isotropic cold-drawing is proposed which allows to reproduce the tests. The parameters of this behaviour law are identified on a 1D creep test in drained conditions. Thus, the delayed deformations come from the poro-elastic and visco-plastic behaviour of the rock. It is shown that both phenomena can be separated. All poro-elasto-visco-plastic parameters are identified and a semi-explicit solution of the creep test is used. Chapter 6 presents an identification method of the elasto-visco-plastic parameters for the general case. The identification is equivalent to the minimization of a cost functional. The gradient of the functional is calculated by direct differentiation. The direct differentiation method is developed in Cast3M for the Norton-Hoff elasto-visco-plastic law. The identification of the parameters of this law on 1D and 2D configurations of underground facilities is then presented. Measurements are simulated using a direct calculation, eventually disturbed. The unicity and stability of this identification problem are discussed. In chapter 7, the method is applied to a real case: the Chamoise tunnel. 1D and 2D flat deformation modeling is used to solve the direct problem. The modeling hypotheses are justified and the obtained parameters allow to correctly reproduce the data. (J.S.)

  2. Creep and damage in argillaceous rocks: microstructural change and phenomenological modeling; Fluage et endommagement des roches argileuses: evolution de la microstructure et modelisation phenomenologique

    Energy Technology Data Exchange (ETDEWEB)

    Fabre, G

    2005-06-15

    The underground radioactive waste disposal far exceeds the period of exploitation of common civil engineering works. These specific projects require to predict the irreversible deformations over a large time scale (several centuries) in order to assess the extension and to forecast the evolution of the EDZ (Excavation Damage Zone) around the cavity. In this study, the viscosity of three sedimentary argillaceous rocks has been studied under different conditions of uniaxial compression: static or cyclic creep tests, monotonic and quasistatic tests, performed across various strata orientations. Argillaceous rocks are studied as a possible host layer for radioactive waste disposals. Indeed, they present some of the physical characteristics and mechanical properties, which are essential for being a natural barrier: low permeability, high creep potential and important holding capacity of radioactive elements. The purpose of the experimental study was to shed some light over the mechanisms governing the development of delayed deformations and damage of argillaceous rocks. It relates three rocks: an argillite from East of France, a Tournemire argillite and a marl from Jurassic Mountains. On atomic scale, viscoplastic deformations are due to irreversible displacements of crystalline defects, called dislocations. The experimental study was also supplemented with observations on thin sections extracted from the argillite and marl samples using a SEM. The aim was to identify the mechanisms responsible for the time-dependent behaviour on a microstructural scale. Analytical simulations of the mechanical behaviour of the three rocks gave parameters used in different viscoplastic models. The best modeling was obtained with the viscoplastic model which takes account of the development of volumetric strains and of the damage anisotropy. (author)

  3. Dynamics of stellar wind in a Roche potential: implications for (i) outflows & periodicities relevant to astronomical masers, and (ii) generation of baroclinicity

    CERN Document Server

    Singh, Nishant K

    2015-01-01

    We study the dynamics of stellar wind from one of the bodies in the binary system, where the other body interacts only gravitationally. We focus on following three issues: (i) we explore the origin of observed periodic variations in maser intensity; (ii) we address the nature of bipolar molecular outflows; and (iii) we show generation of baroclinicity in the same model setup. From direct numerical simulations and further numerical modelling, we find that the maser intensity along a given line of sight varies periodically due to periodic modulation of material density. This modulation period is of the order of the binary period. Another feature of this model is that the velocity structure of the flow remains unchanged with time in late stages of wind evolution. Therefore the location of the masing spot along the chosen sightline stays at the same spatial location, thus naturally explaining the observational fact. This also gives an appearance of bipolar nature in the standard position-velocity diagram, as has ...

  4. Prevalence of human papillomavirus in 5,072 consecutive cervical SurePath samples evaluated with the Roche cobas HPV real-time PCR assay

    DEFF Research Database (Denmark)

    Preisler, Sarah; Rebolj, Matejka; Untermann, Anette;

    2013-01-01

    New commercially available Human Papillomavirus (HPV) assays need to be evaluated in a variety of cervical screening settings. Cobas HPV Test (cobas) is a real-time PCR-based assay allowing for separate detection of HPV genotypes 16 and 18 and a bulk of 12 other high-risk genotypes. The aim...... of the present study, Horizon, was to assess the prevalence of high-risk HPV infections in an area with a high background risk of cervical cancer, where women aged 23-65 years are targeted for cervical screening. We collected 6,258 consecutive cervical samples from the largest cervical screening laboratory...... in Denmark serving the whole of Copenhagen. All samples were stored in SurePath media. In total, 5,072 samples were tested with cobas, Hybrid Capture 2 High Risk HPV DNA test (HC2) and liquid-based cytology. Of these, 27% tested positive on cobas. This proportion decreased by age, being 43% in women aged 23...

  5. Characterization of Rock Wettability Though Dielectric Measurements Caractérisation de la mouillabilité des roches au moyen de mesures diélectriques

    Directory of Open Access Journals (Sweden)

    Bona N.

    2006-12-01

    Full Text Available The wettability of glass filters and Berea sandstone was investigated using the electric response in the interval 10² - 10 (to the power of 8 Hz. The natural wettability of the materials was modified to get two different sets of samples, one with strong water and the other with strong oil wettability. The samples were saturated to various degrees up to 40% with deionized water or brine. Measurements showed that the electric responses of water-wet and oil-wet samples were markedly different and more complex than those predicted by two standard models. The dispersivity and the loss tangent were found to be the most suitable parameters to check the wettability of the samples. La mouillabilité de filtres de verre et de grès de Béréa a été caractérisée par leur réponse électrique dans l'intervalle 10² - 10 puissance 8 Hz. Au moyen de traitements appropriés, la mouillabilité naturelle des matériaux a été modifiée afin d'obtenir deux séries différentes d'échantillons ayant respectivement de fortes mouillabilités à l'eau et à l'huile. Les échantillons ont été saturés à des degrés variés (pas plus de 40 % avec de l'eau permutée ou de la saumure. Les mesures ont montré que les réponses électriques des échantillons mouillables à l'eau ou mouillables à l'huile étaient nettement différentes et plus compliquées que celles prédites par deux modèles standard. En outre, on a pu constater que la dispersivité et la tangente de pertes constituent les paramètres les plus pertinents pour caractériser la mouillabilité des échantillons.

  6. Fractures inside crystalline rocks. Effects of deformations on fluid circulations; Fractures dans les roches cristallines. Effets des deformations sur les circulations de fluides

    Energy Technology Data Exchange (ETDEWEB)

    Gentier, S

    2005-07-01

    The modeling of fluid flows inside granite massifs is an important task for the evaluation of the feasibility of radioactive waste storage inside such formations. This document makes a synthesis of the works carried out since about 15 years, in particular by the French bureau of geological and mining research (BRGM), about the hydro-mechanical behaviour of a fracture and about the hydrodynamical characterization of fracture networks inside crystalline rocks: 1 - introduction; 2 - hydro-mechanical behaviour under normal stress: experimental results (hydro-mechanical behaviour, flow regimes, mechanical behaviour, test protocol, complementary tests, influence of samples size), geometrical interpretation of experimental results (relation with walls geometry, relation with voids geometry, relation with contacts geometry), hydro-mechanical modeling (hydraulic modeling, mechanical modeling); 3 - from the hydro-mechanical behaviour under normal stress to the coupling with heat transfers and chemistry: experiment for the study of the chemo-thermo-hydro-mechanical coupling (experimental results, relation with walls morphology), thermo-hydro-mechanical experiments, thermo-hydro-chemical experiments with fractures, conclusions; 4 - hydro-mechanical behaviour during shear: experimental results, geometrical interpretation (relation with the geometry of damaged zones, relation with voids geometry, relation with walls geometry), hydro-mechanical modeling (mechanical modeling, hydro-mechanical modeling of the behaviour during shear). (J.S.)

  7. Benefit of Hepatitis C Virus Core Antigen Assay in Prediction of Therapeutic Response to Interferon and Ribavirin Combination Therapy

    OpenAIRE

    Takahashi, Masahiko; Saito, Hidetsugu; Higashimoto, Makiko; Atsukawa, Kazuhiro; Ishii, Hiromasa

    2005-01-01

    A highly sensitive second-generation hepatitis C virus (HCV) core antigen assay has recently been developed. We compared viral disappearance and first-phase kinetics between commercially available core antigen (Ag) assays, Lumipulse Ortho HCV Ag (Lumipulse-Ag), and a quantitative HCV RNA PCR assay, Cobas Amplicor HCV Monitor test, version 2 (Amplicor M), to estimate the predictive benefit of a sustained viral response (SVR) and non-SVR in 44 genotype 1b patients treated with interferon (IFN) ...

  8. Survival of infants born to HIV-positive mothers, by feeding modality, in Rakai, Uganda.

    Directory of Open Access Journals (Sweden)

    Joseph Kagaayi

    Full Text Available BACKGROUND: Data comparing survival of formula-fed to breast-fed infants in programmatic settings are limited. We compared mortality and HIV-free of breast and formula-fed infants born to HIV-positive mothers in a program in rural, Rakai District Uganda. METHODOLOGY/PRINCIPAL FINDINGS: One hundred eighty two infants born to HIV-positive mothers were followed at one, six and twelve months postpartum. Mothers were given infant-feeding counseling and allowed to make informed choices as to whether to formula-feed or breast-feed. Eligible mothers and infants received antiretroviral therapy (ART if indicated. Mothers and their newborns received prophylaxis for prevention of mother-to-child HIV transmission (pMTCT if they were not receiving ART. Infant HIV infection was detected by PCR (Roche Amplicor 1.5 during the follow-up visits. Kaplan Meier time-to-event methods were used to compare mortality and HIV-free survival. The adjusted hazard ratio (Adjusted HR of infant HIV-free survival was estimated by Cox regression. Seventy-five infants (41% were formula-fed while 107 (59% were breast-fed. Exclusive breast-feeding was practiced by only 25% of breast-feeding women at one month postpartum. The cumulative 12-month probability of infant mortality was 18% (95% CI = 11%-29% among the formula-fed compared to 3% (95% CI = 1%-9% among the breast-fed infants (unadjusted hazard ratio (HR = 6.1(95% CI = 1.7-21.4, P-value < 0.01. There were no statistically significant differentials in HIV-free survival by feeding choice (86% in the formula-fed compared to 96% in breast-fed group (Adjusted RH = 2.8[95%CI = 0.67-11.7, P-value = 0.16] CONCLUSIONS/SIGNIFICANCE: Formula-feeding was associated with a higher risk of infant mortality than breastfeeding in this rural population. Our findings suggest that formula-feeding should be discouraged in similar African settings.

  9. Post-Skinnerian, Post-Skinner, or Neo-Skinnerian? Hayes, Barnes-Holmes, and Roche's Relational Frame Theory: A Post-Skinnerian Account of Human Language and Cognition

    Science.gov (United States)

    Ingvarsson, Einar T.; Morris, Edward K.

    2004-01-01

    The perceived inability of behaviorism to deal with complex human behavior has been a recurrent theme among its critics. Although ingenious and subtle, even Skinner's Verbal Behavior (1957) is widely faulted on these grounds, in particular, for failing to explain linguistic generativity (Chomsky, 1959). In Relational Frame Theory: A…

  10. Development of a X-ray micro-tomograph and its application to reservoir rocks characterization; Developpement d`un microtomographe X et application a la caracterisation des roches reservoirs

    Energy Technology Data Exchange (ETDEWEB)

    Ferreira de Paiva, R.

    1995-10-01

    We describe the construction and application to studies in three dimensions of a laboratory micro-tomograph for the characterisation of heterogeneous solids at the scale of a few microns. The system is based on an electron microprobe and a two dimensional X-ray detector. The use of a low beam divergence for image acquisition allows use of simple and rapid reconstruction software whilst retaining reasonable acquisition times. Spatial resolutions of better than 3 microns in radiography and 10 microns in tomography are obtained. The applications of microtomography in the petroleum industry are illustrated by the study of fibre orientation in polymer composites, of the distribution of minerals and pore space in reservoir rocks, and of the interaction of salt water with a model porous medium. A correction for X-ray beam hardening is described and used to obtain improved discrimination of the phases present in the sample. In the case of a North Sea reservoir rock we show the possibility to distinguish quartz, feldspar and in certain zone kaolinite. The representativeness of the tomographic reconstruction is demonstrated by comparing the surface of the reconstructed specimen with corresponding images obtained in scanning electron microscopy. (author). 58 refs., 10 tabs., 71 photos.

  11. NMR studies of the molecules dynamics to the solid-liquid interfaces: from graded porous materials to oil rocks; Etudes RMN de la dynamique des molecules aux interfaces solide-liquide: des materiaux poreux calibres aux roches petroliferes

    Energy Technology Data Exchange (ETDEWEB)

    Godefroy, S.

    2001-11-01

    Low field NMR relaxation for laboratory or in-situ applications provides critical information for oil recovery such as porosity, saturation, and permeability of rocks. In addition, pore size distribution and wettability can also be obtained in some cases. The technique relies on the measurement of proton longitudinal (T{sub 1}) or transverse (T{sub 2}) nuclear relaxation times. For better predictions, the surface micro-dynamics and the chemical properties of the liquids entrapped in the pore space are important and must be characterized. It is well known that the NMR relaxation is enhanced by the paramagnetic impurities at the pore surface but many other parameters influence the relaxation time distributions. These parameters are used to derive the petrophysical properties of the rocks. We propose here an original method to probe the dynamics of water and oil at the pore surface. In the present study, we used both nuclear relaxation at 2.2 MHz and field cycling Nuclear Magnetic Relaxation Dispersion (NMRD) techniques. We applied these two techniques to different kinds of water or oil saturated macroporous media (grain packings, outcrop and reservoir rocks with SiO{sub 2} or CaCO{sub 3} surfaces). We studied the dependence of NMR relaxation on pore size, magnetic field and temperature. Varying the pore size and the surface density of paramagnetic impurities of water saturated grain packings allowed experimental evidence for the two limiting regimes of the water relaxation in pores (surface- and diffusion-limited regimes). NMRD technique (evolution of 1/T{sub 1} with the magnetic field) allowed us to probe liquid surface dynamics in water or oil fully saturated grain packing, outcrop rocks or reservoir rocks (water- and oil-wet surfaces). We evidenced a two-dimensional molecular surface diffusion and directly estimated important parameters such as correlation times, residence times and molecular self-diffusion on the surface. Finally, we proved that the temperature dependence of T{sub 1} and T{sub 2} is directly related to the surface chemistry of the rocks. Such a dependence is clearly important for oil field in-situ measurements (well logging). (author)

  12. Comparative evaluation of Roche Aurora FLOW, Becton and Dickinson Viper system, and Dynex DS2 for detection of Chlamydia trachomatis, Neisseria gonorrhoeae, and Trichomonas vaginalis in various clinical specimens.

    Science.gov (United States)

    Vahidnia, Ali; Costa, Sandra; Veenings, Sanne; Tuin, Hellen; van Loon, Linda; Bliekendaal, Harry

    2014-11-01

    The purpose of this study is to introduce a high-throughput system, Aurora FLOW, for the simultaneous detection of 3 clinically relevant pathogens of sexually transmitted infections. Comparative evaluation with other systems revealed an overall concordance of 97.9% for Chlamydia trachomatis and comparable performance for Neisseria gonorrhoeae and Trichomonas vaginalis.

  13. Feasibility study for the installation of a small hydro electric power plant; Etude de faisabilite. Petite centrale hydro-electrique au lieu dit 'Sous les Roches' a Sonceboz

    Energy Technology Data Exchange (ETDEWEB)

    Tissot, N. [MHyLab, Mini-Hydraulics Laboratory, Montcherand (Switzerland); Hausmann, H. [Hans Hausmann, Bevilard (Switzerland)

    2003-07-01

    This report for the Swiss Federal Office of Energy presents a technical, economical and ecological analysis of the feasibility of a small hydroelectric power plant at Sonceboz, in the Swiss Jura mountains. The power of the planed plant would typically be 500 kW for a water head of about 10 m. The study shows that, compared to one single turbine, a pair of Kaplan type turbines would be operational over a larger range of the river's water flow rate. This solution would be a little bit more costly but offer more flexibility for maintenance. Two maximum water flow rates are considered, 4.5 and 6 m{sup 3}/s respectively. According to the economical study, done with current electric kWh prices, both configurations are viable while the largest flow rate leads to a larger profit. The report is rounded up by a sensitivity analysis considering variations in annual power generation, electro-mechanical and construction cost as well as turbine efficiency. It indicates that this last factor could be crucial for the overall profitability. [French] Ce rapport est le resultat detaille de l'analyse technico-economique et ecologique de faisabilite de la realisation d'une petite centrale hydroelectrique basse chute qui pourrait exploiter une denivellation d'environ 10 m pour une puissance de l'ordre de 500 kW pres de Sonceboz dans le Jura suisse. L'etude montre que le couplage de deux turbines Kaplan offrirait une possibilite de fonctionnement sur une plus grande plage de flux ainsi qu'une plus grande flexibilite de maintenance, ceci pour un cout legerement superieur a l'option a une seule turbine. Les debits de 4.5 et 6 m{sup 3}/s sont consideres. L'etude economique montre que le debit de 6 m{sup 3}/s est plus rentable au prix de vente actuel du kWh. L'article se termine par une analyse de sensibilite sur les incertitudes concernant la production annuelle, les prix de l'electromecanique et du genie civil et le rendement des turbines.

  14. Processus de déformation et diagenèse dans les zones de subduction : impact sur les propriétés mécaniques des roches : Approche expérimentale

    OpenAIRE

    Gadenne, Leslie

    2015-01-01

    The shallow portion of subduction zones (0-10 km depth) has long been considered as unable to store and release seismic energy. However, the detection of very-low frequency earthquakes in this zone, as well as the propagation of the coseismic rupture to the trench during the Tohoku-Oki earthquake, question this hypothesis. The difficulty to assess the seismogenic potential of this shallow portion lies principally in the complexity of the processes that occur in this zone, combining deformatio...

  15. Efficacité de la roche phosphatée de Matongo au travers d'un compostage sur une culture de pomme de terre sur un sol acide de Rabiro (Burundi

    Directory of Open Access Journals (Sweden)

    Van den Berghe, C.

    1993-01-01

    Full Text Available Efficiency of phosphatic rock from Matongo applied in the composting process on potatoes on an acid soil of Rabiro. In the frame of the Cooperation between the CVHA (Cultures Vivrieres de Haute Altitute Project and the Program of Fertilisation of the Agro-systems on Altitude (FAVA of the Faculty of Agricultural Sciences in Burundi, the local phosphatic rock from Matongo has been compared to diammonium-phosphate when added in the composting process. The field trials with potatoes have shown that both phosphate sources have the same fertilizing value when the enriched compost was applied at the dose of 20 t/ha. It is very interesting from agricultural and economical viewpoint to use this phosphatic rock in combination with compost.

  16. Simulation of anions diffusion in Callovo-Oxfordian argillite by description of the rock microstructure; Modelisation de la diffusion des anions dans l'argilite du Callovo-oxfordien par description de la microstructure de la roche

    Energy Technology Data Exchange (ETDEWEB)

    Diaz, N. [CEA Saclay, Dept. de Physico-Chimie (DEN/DANS/DPC/SECR), 91 - Gif sur Yvette (France)

    2008-07-01

    The Callovo-Oxfordian argillite has been proposed as host rock for deep underground radioactive wastes storage. The aim of this study is to determine the report of effective diffusion coefficients De(anion)/De(water) at the micrometer scale considering the spatial distribution of minerals in the rock. (A.L.B.)

  17. Suivi de substitution de fluides dans les roches par corrélation de bruit : Expériences ultrasonores au laboratoire et surveillance continue en cours d’exploitation du sous-sol

    OpenAIRE

    Barbouteau, Sandra

    2014-01-01

    Seismic interferometry, like optic interferometry, studies the interferences phenomena between couples of seismic signals, with the aim of pointing at differences between those signals (Curtis et al. 2006 for instance). The data processing consists, generally, in correlating the recordings between different stations to retrieve the Green's function between these sensors (Derode et al. (2003), Wapenaar et al. (2004), Larose et al. (2006), Sanchez-Sesma et Campillo (2006)). This principle has a...

  18. Umbrella structure and channel-wall stoping in the Cambrian St. Roch Formation, Quebec Appalachians: significance for particle support mechanisms and turbulence development in hyper-concentrated sediment gravity flows

    Science.gov (United States)

    Hesse, Reinhard; Fong, Christopher

    2014-03-01

    Umbrella structure is a newly recognized sedimentary structure associated with large platy clasts in resedimented boulder-bearing pebble conglomerate with a sandy matrix. It consists of a sand rim that lacks pebbles on parts or the entire underside of platy boulders, whereas on the upper side, pebbles are in direct contact with the boulders. The depositing processes were high- to hyper-concentrated sediment gravity flows in a submarine channel or canyon on the Cambrian continental slope of North America bordering the Iapetus Ocean. The structure occurs predominantly where clasts dip moderately in the down-current direction. Based on the association of the structure with slightly forward dipping slabs, it is proposed that these down-current dipping slabs may have been in the process of counter-clockwise rotation that was aborted and may have generated a pressure shadow on the underside enabling the inrush of fluid and the infiltration of sand into the anomalous low-pressure zone. The structure has implications for particle support mechanisms in high- to hyper-concentrated sedimentary gravity flows, in that it redirects attention to the much debated mechanism of dispersive pressure and alternatives. It provides an observable sediment structure that supports dispersive pressure which so far depended on experimental evidence and theoretical arguments alone. Vrolijk and Southard's (1997) concept of a `laminar sheared layer' is here for the first time interpreted as having an upward-moving `free-surface' layer effect during deposition from hyper-concentrated flows. Channel-wall stoping involves unlithified turbiditic spillover sand in the levee sediment of the canyon wall that was washed out by the upper diluted parts of the high-concentration flows coming down the channel and leaving a niche in the wall that was filled with coarser channel-axis facies by the same flow (or later flows) when its aggradation reached the level of the niche. The contact between turbidite and pebble conglomerate occurs now more than 2 m laterally into the exposed channel wall. Channel-wall stoping tracks turbulence development in hyper-concentrated gravity flows.

  19. Mechanical behaviour and rupture in clayey rocks studied by x-ray micro tomography; Comportement mecanique et rupture dans les roches argileuses etudies par micro tomographie a rayons X

    Energy Technology Data Exchange (ETDEWEB)

    Lenoir, N

    2006-03-15

    Within the framework of feasibility studies of underground repositories for radioactive waste, the study of permeability evolution with damage of the host layer is crucial. The goals of this work were: (i) to characterize experimentally the damage of two clayey rocks (BEAUCAIRE MARL and EAST SHALE) with x-ray micro tomography, (ii) to develop a high pressure triaxial set-up adapted to permeability measurement on very low permeability rocks.A number of original triaxial devices have been realised to characterize damage of clayey rocks, under deviatoric loading, with x-ray micro tomography on a synchrotron beamline at the ESRF (Grenoble). Localized damage and its evolution have been characterized at a fine scale (of order of ten microns). Digital image correlation techniques, extended to 3d images, have been used to measure incremental strain fields from tomographic images. we demonstrated that these techniques are very useful in the study of the localized damage of geo-materials and especially for the initiation. A high pressure triaxial device has been realised to measure permeability evolution of the east shale as a function of applied stress (isotropic and deviatoric). The particularity of this set-up is the small size of the test specimen (cylinder of 10 mm in diameter and 20 mm in height) which allows significant reduction of test duration. (author)

  20. An experimental study on mineral sequestration of CO{sub 2} in basics and ultra basics rocks; Etude experimentale des reactions de carbonatation minerale du CO{sub 2} dans les roches basiques et ultrabasiques

    Energy Technology Data Exchange (ETDEWEB)

    Dufaud, F

    2006-11-15

    The first part of the thesis is dedicated to dissolution data of siderite FeCO{sub 3} and magnetite Fe{sub 3}O{sub 4} which have been monitored in situ on the FAME beamline of the european synchrotron radiation facility in Grenoble. Iron in solution close to siderite single crystals is shown to be divalent hydrated. The small size of the experimentally investigated volume of solution (200 *400 micrometer and 3 mm height) allowed to work with single crystals in well defined geometries. No specific interaction was observed between iron (II) and dissolved inorganic carbon, suggesting that modelling siderite evolution under high CO{sub 2} pressures by using CO{sub 2}-less very acidic (pH 1-2) solutions is adequate. Using initial reaction rates, we get an activation energy for siderite dissolution of 62 kJ.mol{sup -1}, consistent with existing literature data. Such a value is suggestive of a mineral/solution interface mechanism.. Data from this study and from literature are consistent over a temperature range 25 C - 125 C and a pH range pH 1-7 with an empirical law: pk = pH + E{sub a}/(ln(10)*RT(K)) - log(S/V) - 10,5 where E{sub a} = 62 kJ.mol{sup -1} and S/V is the ratio between solid surface S and fluid volume V. A value of activation energy of 73.5 kJ.mol{sup -1} is obtained in the case of magnetite, also consistent with mineral/solution processes. The second and major part of the thesis work is the realization of analogical experiments for simulating carbonation of basic and ultra basic minerals. Experiments were carried out on consolidated rock cores at 90 C and 280 bar of CO{sub 2} (low temperature experiments) and on powders contained in metallic capsules at 400-500 C and 1000-1700 bars of CO{sub 2} (high temperature experiments). The rate of mineral storage of CO{sub 2} was defined as the molar ratio of solid carbonate formed over total CO{sub 2} injected. It is of about 1% in three months in low temperature experiments whereas it reaches several tens of percents per hour in high temperature experiments. In all cases, carbonation is shown to proceed according to dissolution/precipitation mechanisms. A quasi stoichiometric coupling is evidenced between carbonation and (proto) serpentinization in high-temperature experiments. Newly formed carbonates are mostly magnesite MgCO{sub 3} with Fe and Ca in solid solution. In low temperature samples, the silicates are covered with a thin silica layer and with carbonate spherules consisting of ankerite CaFe(CO{sub 3}){sub 2} - dolomite CaMg(CO{sub 3}){sub 2} - siderite cores surrounded by magnesite overgrowth. In CO{sub 2}-saturated water, peridotites are more reactive than serpentinite and basalts, in accordance with thermodynamic modelling whereas serpentinites are the most reactive in the supercritical CO{sub 2} phase, showing stronger reactivities in this latter phase than in CO{sub 2}-saturated water. In high temperature experiments, the rate of mineral storage is larger at 400 C than at 500 C, in agreement with thermodynamic modelling of the system. High water fugacities and high fluid salinities are shown to have a positive effect on mineral storage rates. Isotopic mass balance of carbon have evidenced that about 15% of the mineral storage consist of a reduced carbon phase, also identified by transmission electron microscopy as an ill-organized graphite phase. (author)

  1. Geochemical modeling of water-gas-rock interactions. Application to mineral diagenesis in geologic reservoirs; Modelisation geochimique des interactions eau-gaz-roche. Application a la diagenese minerale dans les reservoirs geologiques

    Energy Technology Data Exchange (ETDEWEB)

    Bildstein, O.

    1998-03-13

    The Ph.D. report describes a conceptual and numerical model for simulating gas-water-rock interaction during mineral diagenesis of sediments. The main specific features of this model are the following: applicable to open systems, half-implicit resolution numerical method, feedback on the texture evolution (grain model), existence of a gas phase, oxido-reduction phenomena. (author) 217 refs.

  2. Composite microstructural anisotropies in reservoir rocks: consequences on elastic properties and relation with deformation; Anisotropies microstructurales composites dans les roches reservoir: consequences sur les proprietes elastiques et relation a la deformation

    Energy Technology Data Exchange (ETDEWEB)

    Louis, L.

    2003-10-15

    From diagenesis to tectonic stress induced deformation, rock microstructures always present some anisotropy associated with a preferential orientation, shape or spatial arrangement of its constituents. Considering the consequences anisotropy has on directional transport properties and compliance, as the geological history it carries, this approach has received a particular attention in numerous works. In this work, the microstructural features of various sedimentary rocks were investigated through direct observations and laboratory measurements in naturally deformed and undeformed blocks, samples being considered as effective media. All investigated samples were found to be anisotropic with respect to the physical properties we measured (i.e. ultrasonic P-wave velocity, magnetic susceptibility, electrical conductivity). Considering that P-wave velocities can be described by a second order tensor, we applied to the velocity data the same inversion procedure as the one routinely used in magnetic studies, which provided an efficient tool to estimate and compare these 3D anisotropies with respect to the original sample geographical position. In each case, we tried to identify as thoroughly as possible the microstructural source of the observed anisotropies, first by the mean of existing models, then through direct observations (optic and electronic microscopy). Depending on the rock investigated, anisotropy was found to be controlled by pore shape, intergranular contact distribution, preferentially oriented microcracks interacting with compaction pattern or pressure solution cleavages interacting with each other. The net result of this work is that P-wave velocity anisotropy can express the interaction between different microstructural features as well as their evolution during deformation. (author)

  3. Detecção do DNA de Chlamydia trachomatis em espondiloartropatias e artrite reumatóide Detection of Chlamydia trachomatis DNA in spondyloarthropathies and rheumatoid arthritis

    Directory of Open Access Journals (Sweden)

    Rafael Navarrete Fernandez

    2005-10-01

    Full Text Available Chlamydia trachomatis é a bactéria responsável pela doença sexualmente transmissível mais prevalente no mundo. A maioria das infecções em homens e mulheres é assintomática e, quando não diagnosticada e tratada, pode causar artrite e complicações relacionadas ao aparelho reprodutor feminino. OBJETIVO: pesquisar o DNA de C. trachomatis no líquido sinovial e urina de pacientes com espondiloartropatias e artrite reumatóide (AR, avaliar a presença de anticorpos séricos IgG e IgM anti-C. trachomatis nesses dois grupos de doenças e identificar o antígeno HLA-B27 em pacientes com espondiloartropatias. MÉTODOS: a população do estudo consistiu em 15 pacientes com espondiloartropatias: nove com espondiloartropatia indiferenciada (EI e seis com artrite reativa (ARe (grupo I e 15 pacientes com AR (grupo II. O DNA clamidial foi pesquisado em amostras de líquido sinovial e urina de todos os pacientes, empregando-se a PCR (Amplicor Roche, Suíça. Os anticorpos IgG e IgM anticlamidiais foram quantificados por imunofluorescência indireta (IFI, enquanto o HLA-B27 foi tipado em 15 pacientes do grupo I por citometria de fluxo. RESULTADOS: o DNA da C. trachomatis foi evidenciado apenas em uma amostra de líquido sinovial do grupo I (6,7%, sendo o paciente portador de ARe. Em dois pacientes com AR, o DNA clamidial foi identificado na urina (13,3%. Os anticorpos IgG anticlamidiais estavam presentes em oito pacientes da população estudada, três do grupo I (20% e cinco do grupo II (33,3%. O maior título desse anticorpo (1/256 associou-se com a presença do DNA clamidial na urina de um paciente do grupo II. O anticorpo IgM não foi detectado em nenhuma amostra dos dois grupos. O antígeno HLA-B27 foi positivo em quatro indivíduos do grupo II (26,7% e sua presença relacionou-se com sacroileíte. CONCLUSÕES: os resultados deste estudo indicam que em pacientes com diagnóstico de espondiloartropatias e artrite reumatóide, com quadro articular

  4. Ravimid, risk, raha & tervis / Inge Pitsner

    Index Scriptorium Estoniae

    Pitsner, Inge

    2008-01-01

    Ravimifirma Roche Eesti OÜ tegevjuht Kadri Mägi selgitab ravimiäri toimemehhanisme ning põhjuseid, miks ravimitööstus on riskantne ja kallis valdkond. Lisa: Mis on farmakoökonoomika?; Roche Eesti kontor

  5. Benefit of hepatitis C virus core antigen assay in prediction of therapeutic response to interferon and ribavirin combination therapy.

    Science.gov (United States)

    Takahashi, Masahiko; Saito, Hidetsugu; Higashimoto, Makiko; Atsukawa, Kazuhiro; Ishii, Hiromasa

    2005-01-01

    A highly sensitive second-generation hepatitis C virus (HCV) core antigen assay has recently been developed. We compared viral disappearance and first-phase kinetics between commercially available core antigen (Ag) assays, Lumipulse Ortho HCV Ag (Lumipulse-Ag), and a quantitative HCV RNA PCR assay, Cobas Amplicor HCV Monitor test, version 2 (Amplicor M), to estimate the predictive benefit of a sustained viral response (SVR) and non-SVR in 44 genotype 1b patients treated with interferon (IFN) and ribavirin. HCV core Ag negativity could predict SVR on day 1 (sensitivity = 100%, specificity = 85.0%, accuracy = 86.4%), whereas RNA negativity could predict SVR on day 7 (sensitivity = 100%, specificity = 87.2%, accuracy = 88.6%). None of the patients who had detectable serum core Ag or RNA on day 14 achieved SVR (specificity = 100%). The predictive accuracy on day 14 was higher by RNA negativity (93.2%) than that by core Ag negativity (75.0%). The combined predictive criterion of both viral load decline during the first 24 h and basal viral load was also predictive for SVR; the sensitivities of Lumipulse-Ag and Amplicor-M were 45.5 and 47.6%, respectively, and the specificity was 100%. Amplicor-M had better predictive accuracy than Lumipulse-Ag in 2-week disappearance tests because it had better sensitivity. On the other hand, estimates of kinetic parameters were similar regardless of the detection method. Although the correlations between Lumipulse-Ag and Amplicor-M were good both before and 24 h after IFN administration, HCV core Ag seemed to be relatively lower 24 h after IFN administration than before administration. Lumipulse-Ag seems to be useful for detecting the HCV concentration during IFN therapy; however, we still need to understand the characteristics of the assay.

  6. Hepatitis B virus markers in anti-HBc only positive individuals.

    Science.gov (United States)

    Weber, B; Melchior, W; Gehrke, R; Doerr, H W; Berger, A; Rabenau, H

    2001-07-01

    Isolated reactivity to hepatitis B virus (HBV) core antigen (anti-HBc) is observed relatively frequently in immunocompromised individuals, intravenous drug abusers (IVDA), and in the presence of HCV infection. The reason for the lack of HBsAg is not clear. The aim of the present study was to investigate which factors (genetic variability of S gene, low-level HBsAg, and immune complexes may be responsible for the failure of HBsAg detection with commercial HBsAg screening assays. Dilution series of two recombinant HBsAg escape mutants and dilutions of serum samples from chronic HBV carriers with multiple insertions in the a determinant and different HBsAg subtypes were tested with a highly sensitive assay that detects wild-type HBsAg (Elecsys HBsAg, Roche Diagnostics, Penzberg, Germany) and two assays that detect HBV wild-type and escape mutants (Murex HBsAg Version 3, Murex and Enzygnost HBsAg 5.0, Dade Behring, Marburg, Germany). Elecsys HBsAg showed in comparison to Murex HBsAg Version 3 and Enzygnost HBsAg 5.0 a reduced sensitivity for escape mutant detection. On the other hand, the best performance for HBsAg subtype detection was obtained with Elecsys HBsAg. In the second part of the study, a selected panel of isolated anti-HBc reactive (n = 104) serum samples (AxSYM Core) was submitted to testing by Elecsys HBsAg, Murex HBsAg Version 3, Enzygnost HBsAg 5.0, and HBsAg detection after immune complex dissociation (ICD) and anti-HBs determination with two different assays (AxSYM Ausab and Elecsys Anti-HBs). To assess the specificity of anti-HBc test results, all the samples were tested by a second anti-HBc assay (Elecsys Anti-HBc). Quantitative HBV DNA detection was undertaken with a commercially available HBV PCR assay (Amplicor HBV Monitor). HCV infection was present in 65.4% of anti-HBc only reactive individuals. Five AxSYM Core positive samples were negative by Elecsys Anti-HBc. Overall, 15 (14.4%) AxSYM Ausab negative samples gave positive results with Elecsys

  7. HBV and neurological impairment in HIV-infected patients

    Directory of Open Access Journals (Sweden)

    L Manolescu

    2012-11-01

    Full Text Available Objective: HIV can affect CNS in early stages of disease and determine neurological impairment. HBV DNA was found in CSF of HIV co-infected patients, but little is known about the neurotropic character of this virus. Here we assessed the degree of association between HBV infection and neurological impairment in a large cohort of long-term survivors, HIV-infected patients that experienced multiple therapeutic schemes over time. Methods: A total of 462 HIV-1-infected patients were retrospectively followed up for 10 years for HBV infection and neurological impairment. The patients were tested for immune (flow cytometry and virological parameters of HIV infection (Roche Amplicor, version 1.5/ COBAS AmpliPrep/COBAS TaqMan HIV-1 test and for HBV infection markers (HBsAg, anti HBc: Murex Biotech ELISA tests. Many of these patients have experienced between one and six regimens such as: 2 NRTIs, 3 NRTIs, 2 NRTIs+1 NNRTI, 1 NRTI+1 NNRTI+1 PI, 2 NRTIs+2 PIs. Results: After 10 years 29.87% of the patients presented neurological impairment. Out of them 56.52% were HBV-infected. The prevalence of HIV encephalopathy (HE in our studied cohort was 22.7% and 50.4% of these patients were HBV-infected. The median HIV diagnosis age was 7 and the median age of HE diagnosis was 10. In order to establish a possible correlation between HBV infection and HE we first reviewed and excluded the main risk factors associated with HE at the moment of diagnosis: low weight, anemia, constitutional symptoms, low CD4+count, high plasma HIV-RNA load. No patient was infected with HCV. The groups of patients that presented HE and HBsAg and HE without HBsAg were balanced regarding sex, number of deceased patients, number of class C3 patients, but the patients in first group presented lower CD4 values at HE diagnosis vs patients from second group 2: 44.5 vs 95 cells/µL, p=0.3; lower nadir CD4 count: 38 vs 51 cell/µL, p=0.1; and slightly higher HIV viral load: 5.2 vs 5 log10 copies

  8. Emplacement conditions and age of granitoids from the southern external zone of the French central massif: petro-structural and {sup 40}Ar-{sup 39}Ar geochronological study of rocks from their contact aureole and of some magmatic rocks. Geotectonic implications; Conditions et ages de mise en place des granitoides de la zone externe sud du massif central francais: etude petro-structurale et geochronologique {sup 40}Ar-{sup 39}Ar des roches de leurs aureoles de contact et de quelques roches magmatiques. Implications geotectoniques

    Energy Technology Data Exchange (ETDEWEB)

    Najoui, K

    1996-12-20

    A comparative petro-structural and geochronological {sup 40}Ar/{sup 39}Ar study conducted on three granitoids (Mont-Lozere, Sidobre and Aigoual/St-Guiral/Liron) outcropping in the south of the French Massif Central allows us to specify the conditions of their emplacement in the external area of the Hercynian belt. The local gravimetric study shows that the St-Guiral and the Liron have laccolitic shapes and are asymmetric. The paragenetic analysis of the mineralogical associations which occur in the three contact aureoles, the metamorphic gradients and P-T estimates show that the Aigoual, the St-Guiral, the Liron and the Pont-de-Montvert (Mont-Lozere) granodiorites (T: 650-700 deg C; P: 1-1.5 kb) were emplaced in more superficial levels than the early basic and early granodiorite magmas of the St-Guiral, and the Sidobre granite (T: 550-600 deg C; P: 2-2.5 kb). Besides, the structural observations in the host rocks show that emplacement of the St-Guiral granodiorite is synchronous with the deformation and regional metamorphism whereas the Liron granodiorite emplaced later. The laser-probe dating of single biotites and hornblendes corroborate this fact. The {sup 40}Ar/{sup 39}Ar data of the St-Guiral hornfels and granodiorites give ages of 324 Ma. The Liron mafic micro-granular enclaves and lamprophyres give ages of 310 Ma. This suggests that the early granodiorite magmas were emplaced in the late Visean compressive events, followed by granodioritic injections synchronous with the Namuro-Westphalian extension 257 refs.

  9. CO{sub 2} storage in deep aquifers. Study in real conditions of cap-rock confinement properties and of their alteration; Stockage du CO{sub 2} dans les aquiferes profonds. Etude en conditions reellesdes proprietes de confinement des roches de couverture et de leur alteration

    Energy Technology Data Exchange (ETDEWEB)

    Bachaud, P.

    2010-12-07

    A promising solution to reduce anthropogenic emissions of greenhouse effect gases consists in the injection and long-term storage of a part of the industrial carbon dioxide discharges in underground formations. These formations must be composed of a reservoir surrounded by tight cap-rocks, which represent the first barrier preventing fluids migration. The characterization of their confining properties and of their evolution in presence of CO{sub 2} is thus a key element regarding a storage site security. This work presents a methodology allowing the measurement of cap-rocks transport parameters and the consequences of an alteration under representative conditions of deep aquifers storage. This methodology was applied to carbonate rocks from the Paris basin. The breakthrough pressure, the diffusion coefficient of CO{sub 2} dissolution products,and the permeability, controlling parameters of leakage mechanisms, were measured before and after alteration of the materials by reaction with a CO{sub 2}-saturated brine under reservoir thermodynamic conditions (about 80 C and 100 bar). Results revealed a satisfactory global behaviour under these aggressive conditions, but also a strong diminution of the confinement potential in presence of initial structural faults (sealed fractures, large-diameter pores...) forming higher-permeability zones. A numeric simulation describing the evolution of a homogeneous rock formation during 1000 years was also realized based on parameters directly measured or obtained by modelling of the alteration experiments. It showed that the transformations brought by the CO{sub 2} storage under a rock formation with no initial faults remain very localized spatially. (author)

  10. From Injectivity to Integrity Studies of CO{sub 2} Geological Storage Chemical Alteration Effects on Carbonates Petrophysical and Geomechanical Properties; Caracterisation de l'injectivite et de l'integrite d'un stockage geologique de CO{sub 2}: Effets d'une alteration chimique sur les proprietes petrophysiques et geomecaniques des roches carbonatees

    Energy Technology Data Exchange (ETDEWEB)

    Bemer, E.; Lombard, J.M. [Institut francais du petrole, IFP, 92 - Rueil-Malmaison (France)

    2010-05-15

    The technical and economical success of a CO{sub 2} geological storage project requires the preservation of the site injectivity and integrity properties over its lifetime. Unlike conventional hydrocarbon gas injection, CO{sub 2} injection implies geochemical reactions between the reactive brine and the in situ formations (reservoir and cap rock) leading to modifications of their petrophysical and geomechanical properties. This paper underlines the experimental difficulties raised by the low permeability of samples representative either of the cap rock itself or at least of transition zones between the reservoir and the effective cap rock. Acidification effects induced by CO{sub 2} injection have been studied using an experimental procedure of chemical alteration, which ensures a homogeneous dissolution pattern throughout the rock sample and especially avoids any worm-holing process that would lead to erroneous measurements at the core scale. Porosity, permeability and geomechanical properties of outcrop and field carbonate samples of various permeability levels have been measured under their native state and different levels of alteration. The present work has been conducted within the framework of ANR GeoCarbone-INJECTIVITY and GeoCarbone-INTEGRITY projects. Each experimental step: chemical alteration, petrophysical measurements and geomechanical testing, is considered from the point of view of injectivity and integrity issues. The obtained experimental data show clear trends of chemically induced mechanical weakening. (authors)

  11. Erosion of the Alps: use of Rb-Sr isotopic data from molassic sediments to identify the ages of the metamorphism recorded by the eroded rocks; Erosion des Alpes: histoire metamorphique des roches erodees par l`analyse du couple Rb-Sr des sediments molassiques

    Energy Technology Data Exchange (ETDEWEB)

    Henry, P.; Deloule, E. [Centre National de la Recherche Scientifique (CNRS), 54 - Nancy (France). Centre de Recherches Petrographiques et Geochimiques; Michard, A. [Aix-Marseille-3 Univ., 13 - Marseille (France)

    1994-06-01

    Rb-Sr isotopic data from Oligocene and Miocene peri-alpine molassic sediments allow us to identify the different periods for which the eroded rocks have or have not recorded an alpine metamorphism. The Chattian and the Burdigalian sediments result from the erosion of rocks for which the latest metamorphic event was variscan, while the Stampian, Aquitanian and ``Helvetian`` sediments show evidence for the erosion of rocks which have recorded alpine metamorphic events. The application of this method to old detrital sediments could permit determination of the ages of the tectonic events which occurred in the sediment source regions. (authors). 18 refs., 6 figs.

  12. Temperature effect on the poro-mechanical or hydraulic behaviour of a carbonated rock and a mortar: experimental studies; Influence de la temperature sur le comportement poromecanique ou hydraulique d'une roche carbonatee et d'un mortier: etudes experimentales

    Energy Technology Data Exchange (ETDEWEB)

    Lion, M

    2004-07-15

    The main objective of this study is to evaluate the temperature effect on the hydraulic and poro-mechanical behaviour of a limestone. Many experimental tests (porosity and permeability measurements, uniaxial and hydrostatic compressions tests) were carried out in order to study the thermal treatments effect and so the thermal microcracking effect on rock behaviour. Moreover, an experimental device for permeability measurements under high temperatures (until 200 C) was realized. This experimental device permitted to study the permeability variation of the limestone under thermal stresses. Finally, the behaviour of cementitious materials was studied; the temperature effect on the permeability of a mortar was examined. (author)

  13. Reduction of the greenhouse effect by geological mineral in-situ sequestration of CO{sub 2} in basic rocks: bibliographic synthesis and possibilities in France. Final report; Reduction de l'effet de serre par sequestration geologique minerale in-situ de CO{sub 2} au sein de roches basiques: synthese bibliographique et revue des potentialites en France. Rapport final

    Energy Technology Data Exchange (ETDEWEB)

    Marechal, J.C.; Lachassagne, P

    2004-07-01

    The report constitutes a first bibliographic study defining the environments the most adapted to the geological mineral in-situ sequestration of CO{sub 2}. For each environment the lithology and the rocks permeability and porosity are analyzed. Thus the possible rocks and deposits in France are presented. (A.L.B.)

  14. Numerical modeling of damage and permeability interaction in rocks. Application for the radioactive waste storage in deep galleries; Modelisation numerique du couplage entre l'endommagement et la permeabilite des roches. Application a l'etude des ouvrages de stockage souterrain

    Energy Technology Data Exchange (ETDEWEB)

    Maleki, K

    2004-03-15

    The relation between damage and permeability in rocks is a very important subject in industrial applications. It is for example the case of cracks around the radioactive waste storage galleries (EDZ) which can rise considerably the permeability and so make a serious problem for the sealing and the safety of these structures. The same phenomena can occur in the surrounding concrete wall of the nuclear power stations and also in the cracking of the oil-bearing rocks reservoirs. The experimental research on this subject, specially in laboratory G.3S-LMS in Ecole Polytechnique made it possible to determine the orders of damage effect on the permeability change. But a numerical modeling of these phenomena for setting a constitutive behavior law was to be done. This is the principal objective of this thesis. In this project, at first the notion of crack damage is defined. This type of damage is modeled by the disc shaped crack distribution in the 3D space. The disc's geometrical characteristics (radius, direction and opening) obey the statistical distribution laws, depending on the type of loading (compression or extension). The upper and lower limits of the characteristics are fixed according to actual observations, carried out on clay-stone (host rock selected for the realization of an underground research laboratory in Bure). In order to modeling the damage in the porous media, the double porosity concept is considered. A method of homogenization is used to simulate the flow through the network of cracks and porosity. It allows to derive the equivalent permeability of the fractured porous media. The study of the correlations between permeability and damage, obtained by this method for various values of fracture density, made it possible to obtain a relation between permeability and crack damage, for classical types of loading as simple extension and simple compression. To generalize this relation in the case of any specified triaxial loading, the crack damage is put in relation with the notion of mechanical damage, resulting from a mechanical model connecting the damage to stress and strain. Finally, the poro-mechanical behavior law with damage is implemented to the finite elements computer program ESAR-LCPC The modeling of an underground gallery proved that damage calculation by this method is feasible and allows to compute the evolution of the permeability and the variation of the flow entering the gallery as a function of this damage. (author)

  15. The natural seismic hazard and induced seismicity of the european HDR (hot dry rock) geothermal energy project at Soultz-sous-Forets (Bas-Rhin, France); Alea sismique naturel et sismicite induite du projet geothermique europeen RCS (roche chaude seche) de Soultz-sous-Forets (Bas-Rhin, France)

    Energy Technology Data Exchange (ETDEWEB)

    Helm, J.A.

    1996-06-07

    Development of the Soultz-sous-Forets HDR (Hot Dry Rock) geothermal energy project will involve important fluid injections which will induce micro-seismic events. This thesis discusses the natural seismicity of the region and induced seismicity associated with fluid injections. A catalogue of all historical and instrument seismicity of the Soultz-sous-Forets (SSF) region has been compiled. This seismicity does not correspond to movements along the major tectonic features of the region. The area around SSF has been identified as being one where high heat flow corresponds to low seismicity. The largest well documented seismic event in the region which took place in 1952 had an epicentral intensity of VI. All important data pertaining to the series of seismic events which took place in the region from August to October 1952 have been collected and are presented. This work details the installation and operation of a permanent 3 station network of accelerometers and seismometers around the HDR site. Also the installation and operation of a mobile network of vertical seismometers during fluid injections. 167 micro-seismic events were recorded on the surface network, with magnitudes from -0.5 to 1.9. The preferential alignment of the micro-seismic cloud is N160 deg. Individual focal mechanisms of the larger seismic events correspond to an extensional tectonic regime. Stress inversion of P wave polarities indicates that the maximum stress is vertical and the intermediate and minimum stress axes horizontal. The largest of the horizontal stresses is orientated N124 deg and the smallest N34 deg. Induced seismic movement is taking place on pre-existing fractures controlled by the in situ stress seismic movement is taking place on pre-existing tectonic fractures controlled by the in situ stress field, and the largest of the induced events had a magnitude 1.9. This level of seismicity does not pose any environmental hazard to the region around Soultz-sous-Forets. (author) 151 refs.

  16. Experimental study of chemical-mechanical coupling during percolation of reactive fluid through rocks under stress, in the context of the CO{sub 2} geological sequestration; Etude experimentale du couplage chimie-mecanique lors de la percolation d'un fluide reactif dans des roches sous contrainte, dans le contexte de la sequestration geologique du CO{sub 2}

    Energy Technology Data Exchange (ETDEWEB)

    Le Guen, Y

    2006-10-15

    CO{sub 2} injection into geological repositories will induce chemical and mechanical instabilities. The study of these instabilities is based on experimental deformation of natural rock samples under stress, in the presence of fluids containing, or not, dissolved CO{sub 2}. Triaxial cells used for the experiments permitted an independent control and measurement of stress, temperature, fluid pressure and composition. Vertical strains were measured during several months, with a resolution of 1.10{sup -12} s{sup -1} on the strain rate. Simultaneously, fluids were analysed in order to quantify fluid-rock interactions. For limestone samples, percolation of CO{sub 2}-rich fluids increases strain rate by a factor 1.7 up to 5; on the other hand, sandstone deformation remained almost the same. Increase in strain rate with limestone samples was explained by injected water acidification by the CO{sub 2} which increases rock solubility and reaction kinetics. On the opposite, small effect of CO{sub 2} on quartz explains the absence of deformation. X-ray observations confirmed the importance of rock composition and structure on the porosity evolution. Numerical simulations of rock elastic properties showed increasing shear stress into the sample. Measured deformation showed an evolution of reservoir rocks mechanical properties. It was interpreted as the consequence of pressure solution mechanisms both at grains contacts and on grain free surfaces. (author)

  17. POCT血糖仪与罗氏P-800全自动生化仪对血糖测定结果的比对分析%Comparative Analysis on Determination Results of Blood-glucose between POCT Blood Glucose Meter and Roche P-800 Full Automatic Biochemical Analyzer

    Institute of Scientific and Technical Information of China (English)

    罗莞超; 陈斯亮; 龚宇锋

    2012-01-01

    目的 通过比对实验,验证POCT血糖仪与罗氏p-800检测血糖所得的数据的相关性与偏倚,观察两者差异与POCT血糖仪的结果能否被临床接受.方法 以罗氏P-800测定血清葡萄糖为标准方法,POCT血糖仪测定末稍血全血血糖为实验方法,检测由心内科、肾内科、呼吸内科提供的各20例标本,所得结果进行统计学分析.结果 经过统计学分析,在心内科、肾内科使用的POCT血糖仪符合比对要求,与罗氏P-800的相关性良好,相关系数r>0.95,偏倚符合卫生部要求;呼吸内科使用的POCT血糖仪不符合比对要求.结论 POCT血糖仪虽简便、快速,但需规范管理,以保证检测结果的准确性.

  18. Sequence analysis and genotyping of genital Chlamydia trachomatis among patients with suspected-Neisseria gonorrhoeae%拟诊为淋病患者中泌尿生殖道沙眼衣原体基因分型及序列分析

    Institute of Scientific and Technical Information of China (English)

    张娟娟; 卢次勇; 冯铁建; 赵广录; 张丽君; 王峰; 洪福昌; 蓝丽娜; 吴肖冰; 陶小华; 张春来

    2010-01-01

    目的 了解深圳市拟诊为淋病患者中泌尿生殖道沙眼衣原体的合并感染情况及其基因型分布和序列变异特点.方法 采集401例拟诊为淋病患者的泌尿生殖道分泌物样本,应用Roche Amplicor全自动核酸检测系统对样本进行淋球菌和沙眼衣原体双检,提取DNA,应用巢式聚合酶链反应(nested-PGR)扩增沙眼衣原体主要外膜蛋白基因(omp1)中的VS1~VS2片段,并对其进行序列测定,所获得的序列利用Mega4.0软件与标准参考株进行比对,分析确定其基因型及序列变异情况.结果 401例拟诊为淋病患者中淋球菌的感染率为82.3%(330/401),沙眼衣原体的感染率为24.2%(97/401),淋球菌和沙眼衣原体的合并感染率为21.7%(87/401).97份沙眼衣原体阳性样本中获得73份沙眼衣原体基因片段序列,共检出8个基因型,分别为E型(27.4%)、G/Ga型(23.3%)、D/Da型(16.4%)、F型(13.7%)、J型(11.0%)、H型(5.5%)、B和K型(各1.4%).序列分析发现3例(4.1%)菌株发生错义突变,分别为D/Da型、E型、G/Ga型;F型、H型、J型和K型序列虽多见碱基突变,但均为同义突变.结论淋病患者合并感染沙眼衣原体的比例较高,且泌尿生殖道沙眼衣原体的基因型以E、G/Ga、D/Da和F型为主.序列分析可以为泌尿生殖道沙眼衣原体的分子流行病学研究提供依据.%Objective To understand the prevalence rate of genital Chlamydia trachomatis among a population with suspected-Neisseria gonorrhoeae infection,the distribution of Chlamydia trachomatis genotypes,assess changes in omp1 sequences among patients with Neisseria gonorrhoeae and Chlamydia trachomatis coinfections.Methods Four hundred and one swabs were collected.Chlamydia trachomatis and Neisseria gonorrhoeae were detected by Roche Amplicor System.DNA were extracted from those samples and were amplified by nested PCR.PCR products were sequencing and analyzed by software Mega4.0.Results The prevalence of genital Chlamydia

  19. Comparison of Three Nucleic Acid Amplification Assays of Cerebrospinal Fluid for Diagnosis of Cytomegalovirus Encephalitis

    Science.gov (United States)

    Bestetti, Arabella; Pierotti, Chiara; Terreni, Mariarosa; Zappa, Alessandra; Vago, Luca; Lazzarin, Adriano; Cinque, Paola

    2001-01-01

    The diagnostic reliabilities of three cytomegalovirus (CMV) nucleic acid amplification assays of cerebrospinal fluid (CSF) were compared by using CSF samples from human immunodeficiency virus-infected patients with a postmortem histopathological diagnosis of CMV encephalitis (n = 15) or other central nervous system conditions (n = 16). By using a nested PCR assay, the quantitative COBAS AMPLICOR CMV MONITOR PCR, and the NucliSens CMV pp67 nucleic acid sequence-based amplification assay, sensitivities were 93.3, 86.6, and 93.3%, respectively, and specificities were 93.7, 93.7, and 87.5%, respectively. The COBAS AMPLICOR assay revealed significantly higher CMV DNA levels in patients with diffuse ventriculoencephalitis than in patients with focal periventricular lesions. PMID:11230445

  20. Is real-time PCR better than conventional PCR for Mycobacterium tuberculosis complex detection in clinical samples?

    Science.gov (United States)

    Tortoli, Enrico; Urbano, Pasquale; Marcelli, Fiorella; Simonetti, Tullia M; Cirillo, Daniela M

    2012-08-01

    Cobas Amplicor MTB and later Cobas TaqMan MTB were used to test a very large series of consecutive specimens received for tuberculosis diagnosis. Performance parameters were estimated and compared overall and for separate specimen categories. Both systems showed excellent specificity, and that of TaqMan was the higher. The sensitivities were similar but satisfactory only with respiratory specimens and smear-positive samples.

  1. The Cobas® EGFR Mutation Test v2 assay.

    Science.gov (United States)

    Brown, Paul

    2016-02-01

    Paul Brown speaks to Gemma Westcott, Commissioning Editor: Paul Brown has served as the Head of Roche Molecular Diagnostics at Roche Diagnostics Corporation since February 2010 having previously held a variety of positions within Roche Pharma. After completing his doctorate in organic chemistry he was awarded a post-doctoral fellowship at the California Institute of Technology in Pasadena, CA, USA, but soon returned to his native UK to join Roche Pharma Research. Paul's first post within Roche was as group leader, doing drug discovery and making new small molecule drugs, but later moved into the business part of the company. Since then, he has enjoyed roles such as lifecycle leader for brands such as Tamiflu(®) and Xenical, vice president of sales and marketing of the pharmaceutical division and most recently general manager of Roche Pharma, Sweden. PMID:26838018

  2. Détermination et origine géologique de la matière première de l'industrie lithique du site magdalénien du Rocher de la Caille.

    OpenAIRE

    Alix, Philippe; De Beaune, Sophie A.; Deloge, Huguette; Santallier, Danielle

    2003-01-01

    Les matières premières sélectionnées par les occupants du site magdalénien du Rocher de la Caille appartiennent aux deux grandes catégories des roches sédimentaires et des roches cristallines. Quelques analyses micrographiques sur lames minces ont été effectuées sur des roches volcaniques. Elles étaient majoritairement d'origine locale, ramassées à proximité du site ou dans un rayon d'une dizaine de kilomètres (galets en roches cristallines et granitiques provenant des alluvions de la Loire, ...

  3. Integrative Modeling of Caprock Integrity in the Context of CO2 Storage: Evolution of Transport and Geochemical Properties and Impact on Performance and Safety Assessment Modélisation intégrée de l’intégrité des roches de couverture dans le contexte du stockage du CO2 : évolution des propriétés de transport et impact sur les performances et la sûreté du stockage

    Directory of Open Access Journals (Sweden)

    Bildstein O.

    2010-06-01

    Full Text Available The objective of the “Géocarbone-Intégrité” project (2005-2008 was to develop a methodology to assess the integrity of the caprock involved in the geological storage of CO2. A specific work package of the project (WP5 was dedicated to the integration of (1 the phenomenology describing the evolution of the storage system with a focus on the mechanisms occurring in the caprock and at the interface with the caprock, and (2 the data obtained from the investigation of petrographical, geomechanical, and geochemical properties, before and after reaction with CO2-rich solutions, performed in the other work packages (WP1 to WP4. This knowledge was introduced in numerical models and specific safety scenarios were defined in order to assess the performance of the CO2 storage system. The results of the modeling show that the injection of CO2 can potentially have a significant effect on the caprock by changing the porosity due to the dissolution and precipitation of minerals, but that the impact is limited to a zone from several decimeters to several meters of the caprock close to the interface with the reservoir depending on whether the supercritical carbon dioxide (SC-CO2 plume enters into the caprock and if fractures are present at this location. The methodology used in this project can be applied to a pilot site for the injection of CO2 in the Paris Basin. A key aspect of the safety of such a facility will be to look at the coupling of geochemical alteration and the evolution of geomechanical properties in the short and medium terms (several hundreds of years. The challenge for the future will be to structure and apply the safety assessment methodology with an operational finality, in order to support the robustness of the transition step to CGS projects at the industrial scale. Le Volet 5 du projet « Géocarbone-Intégrité » visait à intégrer l’ensemble des mécanismes étudiés dans les quatre premiers volets du projet pour une évaluation de performance des couvertures et une étude de sûreté afin de s’assurer de leur préservation et de leur intégrité sur le long terme (de l’ordre du millénaire. L’objectif est, d’une part, d’aboutir à la construction d’un modèle phénoménologique multi-échelle global, puis à un modèle numérique décrivant le confinement du CO2 par les couvertures, et, d’autre part, de déterminer les performances du confinement en identifiant les processus clés et les paramètres les plus influents. Une première partie du programme a consisté en une intégration spatiale de l’ensemble des données phénoménologiques et structurales disponibles à la suite des travaux réalisés dans les différents volets (WP1 à WP4 et à la définition des scénarios types d’évolution du site de stockage (niveaux réservoirs et encaissants. Ce travail a permis de définir les cas tests à prendre en compte et de réaliser les calculs de performance par rapport aux scénarios d’injection et par rapport aux hétérogénéités majeures identifiées dans les niveaux de confinement (notamment les fractures. Les résultats montrent que l’injection de CO2 peut avoir un effet significatif, en altérant la porosité par dissolution et précipitation de minéraux, mais que l’impact est limité dans l’espace, de quelques décimètres à quelques mètres de l’interface réservoir-couverture, selon que la bulle de CO2 supercritique pénètre ou non dans la couverture et selon la présence ou l’absence de fractures. La prise en compte des résultats issus de l’analyse de sensibilité et l’analyse des incertitudes permettra de conduire des calculs de sûreté plus précis. Appliqués au futur site d’injection, ces calculs permettront d’évaluer la pérennité des propriétés de confinement des couvertures et de valider la qualité de confinement du site de stockage de CO2. Il conviendra notamment d’évaluer l’impact du couplage entre les phénomènes géochimiques et géomécaniques sur le court et moyen terme (de l’ordre de la centaine d’années. Le défi pour l’avenir est de structurer et d’appliquer la méthodologie de l’analyse de sûreté, en mettant en avant la finalité opérationnelle, de manière à assurer la robustesse de la transition vers les projets de CGS à l’échelle industrielle.

  4. Mise en place et cristallisation des massifs grenus et pegmatitoïdiques des volcans de l'Archipel de la Société, Polynésie française: étude texturale, minéralogique et géochimique des roches à texture grossière des îles de Tahiti Nui, Raiatea, Bora Bora et Maupiti

    OpenAIRE

    Clement, Jean Philippe

    2002-01-01

    This first synthetic study of the coarse grained magmatic rocks from the Society islands results in forming two groups within the five sampled granular and pegmatoidic intrusions: i) the wide and lithologically complex plutonic bodies of Tahiti Nui and Raiatea; ii) the three small intrusions of Bora Bora and Maupiti. In Tahiti Nui, the emplacement of the coarse grained body could have been the cause of the volcano destabilisation, which has preceded the gravity landslide collapse of its north...

  5. Complex geometry and real geometry

    Institute of Scientific and Technical Information of China (English)

    YU; Yanlin; ZHU; Lin; FENG; Xiuhong

    2005-01-01

    There is a well-known way to generalize the Riemann-Roch operator for Kahler manifold to that for Hermitian manifold. In this paper we show a slightly different way to get a generalized Riemann-Roch operator, which is just the Dirac operator. The difference between the two operators is that the latter one enables the so-called Pythagoras equalities.

  6. 阿德福韦治疗对慢性乙型肝炎患者Th细胞相关细胞因子水平及HBVDNA的影响%The effect of adefovir dipivoxil therapy on T helper cell cytokines and HBV DNA loads in patients with hepatitis B

    Institute of Scientific and Technical Information of China (English)

    马萍; 宋诗铎; 王磊

    2011-01-01

    Objective To explore the influence of adefovir dipivoxil (ADV) therapy on the cellular immunity by observing the levels of interferon-γ(IFN-γ), interleukin-4 (IL-4) and their relationship with HBV DNA loads in patients with HBeAg-positive chronic hepatitis B at different time before and after treatment.Methods Sera of 30 patients before ADV therapy and 16 weeks ,52 weeks and 132 weeks after ADV therapy respectively were collected in this study.There were 14 complete response cases(group A), 16 incomplete response cases(group B).Sera of 10 healthy people were chosen as control group(group C).Levels of IFN-γ and IL-4 were detected with enzyme-linked immunosorbent assay; HBV DNA loads were detected by ROCHE COBAS AMPLIC)R HBV MONITOR, lower limit was 103 copy/mL.Results The average IFN-γ level in group A was significantly higher than that of group B (P<0.05)and C(P<0.05), there was no significant difference between group B and C(P>0.05);The level of IL-4 of group A decreased after treatment while group B didn't.HBV DNA loads of all groups had no significant correlation with IFN-γ and IL-4 levels before treatment, but dropped obviously at 16th week after treatment; and the increased level of IFN-γ in group A was significantly higher than that of group B(P<0.05); IL-4 levels in group A decreased gradually, but didn't decreased obviously in group B.Conclusion Cellular immune response of patients with hepatitis B was resumed to some extent after ADV treatment.The resumption level was positively related with the decreased level of HBV DNA load.%目的 观察乙型肝炎e抗原(HBeAg)阳性慢性乙型肝炎患者阿德福韦治疗前后不同时相点血清干扰素(IFN)-γ和白细胞介素(IL)-4的水平,以及其与乙型肝炎病毒(HBV)DNA定量的关系,以探讨阿德福韦治疗对机体免疫状态的影响.方法 采集30例阿德福韦治疗前及治疗16周、52周和132周患者的血清,其中完全应答组14例,部分应答组16

  7. Book Reviews

    Directory of Open Access Journals (Sweden)

    Bart Bechtel

    2008-01-01

    Full Text Available Fair Play: The Moral Dilemmas of Spying. By James M. Olson. The Corporate Spy: Industrial Espionage and Counterintelligence in the Multinational Enterprise with Case Studies (Abridged Version by Edward M. Roche.

  8. Disease: H00184 [KEGG MEDICUS

    Lifescience Database Archive (English)

    Full Text Available urements of S-adenosylmethionine and choline. Metabolism 49:1542-7 (2000) PMID:2130...PMID:11145114 Mudd SH, Jenden DJ, Capdevila A, Roch M, Levy HL, Wagner C Isolated hypermethioninemia: meas

  9. 77 FR 42713 - Notice of Intent to Grant an Exclusive License; PadJack, Inc.

    Science.gov (United States)

    2012-07-20

    ... Technology Transfer Program, 9800 Savage Road, Suite 6541, Fort George G. Meade, MD 20755-6541. FOR FURTHER INFORMATION CONTACT: Marian T. Roche, Director, Technology Transfer Program, 9800 Savage Road, Suite...

  10. EPL-i ümarlaud : kõige olulisem on vähi varajane avastamine / Ille Grün-Ots

    Index Scriptorium Estoniae

    Grün-Ots, Ille, 1960-2016

    2007-01-01

    Ümarlaual osalesid TÜ kliinikumi hematoloogia-onkoloogiakliiniku professor Hele Everaus, ravimifirma Roche Eesti OÜ tegevjuht Kadri Mägi, ravifirma GlaxoSmithKline Eesti filiaali juhataja Akshay Mody ja PERH-i keemiaravispetsialist Helis Pokker

  11. Tere tulemast tagasi, uue põlvkonna utopistid! / Veronika Valk

    Index Scriptorium Estoniae

    Valk, Veronika, 1976-

    2011-01-01

    Francois Roche ja Eva Castro tulevikuarhitektuuri käsitlevatest ettekannetest Tallinna arhitektuuribiennaali raames toimunud sümpoosionil. Maketinäitus "Viini eksiilid. Helisev arhitektuur" Heliose kinos. Eksponeeritakse Viini Die Angewandte arhitektuurikoolis (juht Hani Rashid) õppivate eestlaste loomingut

  12. Equilibrium Configurations of Synchronous Binaries: Numerical Solutions and Application to Kuiper-Belt Binary 2001 QG298

    CERN Document Server

    Gnat, Orly

    2010-01-01

    We present numerical computations of the equilibrium configurations of tidally-locked homogeneous binaries, rotating in circular orbits. Unlike the classical Roche approximations, we self-consistently account for the tidal and rotational deformations of both components, and relax the assumptions of ellipsoidal configurations and Keplerian rotation. We find numerical solutions for mass ratios q between 1e-3 and 1, starting at a small angular velocity for which tidal and rotational deformations are small, and following a sequence of increasing angular velocities. Each series terminates at an appropriate ``Roche limit'', above which no equilibrium solution can be found. Even though the Roche limit is crossed before the ``Roche lobe'' is filled, any further increase in the angular velocity will result in mass-loss. For close, comparable-mass binaries, we find that local deviations from ellipsoidal forms may be as large as 10-20%, and departures from Keplerian rotation are significant. We compute the light curves ...

  13. 76 FR 59416 - Senior Executive Service Performance Review Board

    Science.gov (United States)

    2011-09-26

    .... Gramlick, Carl Graves, Margaret Griffin, Robert P., Ph.D Grimm, Michael Gross-Davis, Leslie Grossman, Seth..., William Ratliff, Gerri L. Rhew, Perry J. Robles, Alfonso Roche, William Rogers, Debra Rossides, Gale...

  14. Tähelepanu keskmes on viiruslik hepatiit / Marika Kusnets

    Index Scriptorium Estoniae

    Kusnets, Marika

    2008-01-01

    Ülemaailmsel hepatiidipäeval 19. mail korraldasid Eesti Gastroenteroloogide Selts (EGS) ja Roche Eesti OÜ meediakanalite esindajatele ümarlaua, et aidata teadvustada C-hepatiidi ohtu, testimis- ja ravivõimalusi Eestis

  15. 1st HPV Test for Use with Preservative Fluid

    Science.gov (United States)

    ... https://medlineplus.gov/news/fullstory_159789.html 1st HPV Test for Use With Preservative Fluid Human papillomavirus ... Food and Drug Administration has approved Roche's cobas HPV Test -- the first diagnostic to be used with ...

  16. Sequence Read Archive (SRA)

    Data.gov (United States)

    U.S. Department of Health & Human Services — The Sequence Read Archive (SRA) stores raw sequencing data from the next generation of sequencing platforms including Roche 454 GS System®, Illumina Genome...

  17. Large-scale survey to describe acne management in Brazilian clinical practice

    OpenAIRE

    Seité S; Caixeta C; Towersey L

    2015-01-01

    Sophie Seité,1 Clarice Caixeta,2 Loan Towersey3 1La Roche-Posay Dermatological Laboratories, Asnières, France; 2La Roche-Posay Dermatological Laboratories, Rio de Janeiro, RJ, Brazil; 3AIDS Division, Hospital Municipal Carlos Tortelly, Niterói, RJ, Brazil Background: Acne is a chronic disease of the pilosebaceous unit that mainly affects adolescents. It is the most common dermatological problem, affecting approximately 80% of teenagers between 12 and 18 years of age. Dia...

  18. Large-scale survey to describe acne management in Brazilian clinical practice

    OpenAIRE

    Seite, Sophie

    2015-01-01

    Sophie Seité,1 Clarice Caixeta,2 Loan Towersey3 1La Roche-Posay Dermatological Laboratories, Asnières, France; 2La Roche-Posay Dermatological Laboratories, Rio de Janeiro, RJ, Brazil; 3AIDS Division, Hospital Municipal Carlos Tortelly, Niterói, RJ, Brazil Background: Acne is a chronic disease of the pilosebaceous unit that mainly affects adolescents. It is the most common dermatological problem, affecting approximately 80% of teenagers between 12 and 18 years...

  19. Linear series on metrized complexes of algebraic curves

    OpenAIRE

    Amini, Omid; Baker, Matthew

    2012-01-01

    A metrized complex of algebraic curves is a finite metric graph together with a collection of marked complete nonsingular algebraic curves, one for each vertex, the marked points being in bijection with incident edges. We establish a Riemann-Roch theorem for metrized complexes of curves which generalizes both the classical Riemann-Roch theorem and its graph-theoretic and tropical analogues due to Baker-Norine, Gathmann-Kerber, and Mikhalkin-Zharkov. We also establish generalizations of the se...

  20. Formation of Regular Satellites from Ancient Massive Rings in the Solar System

    OpenAIRE

    Crida, A.; Charnoz, S.

    2013-01-01

    When a planetary tidal disk -like Saturn's rings- spreads beyond the Roche radius (inside which planetary tides prevent aggregation), satellites form and migrate away. Here, we show that most regular satellites in the solar system probably formed in this way. According to our analytical model, when the spreading is slow, a retinue of satellites appear with masses increasing with distance to the Roche radius, in excellent agreement with Saturn's, Uranus', and Neptune's satellite systems. This ...

  1. A clinical, epidemological, laboratorial, histological and ultrasonographical evaluation of anti-HCV EIA-2 positive blood donors Avaliação clínica, epidemiológica, laboratorial, histológica e ultrassonográfica de doadores de sangue anti-HCV EIA-2 positivos

    Directory of Open Access Journals (Sweden)

    Fernando L. GONÇALES JR

    2000-06-01

    RIBA-2 positive subjects, in 37.5% of the indeterminate RIBA-2 donors and in 9% of the negative RIBA-2 donors. Chronic hepatitis has also been observed in 50% of the histopathological exams of the anti-HCV EIA-2 reagent donors which were indeterminate RIBA-2. Among 18 blood donors with minimal changes histopathological exam 11 (61% were HCV-RNA positive. Our blood donors anti-HCV reagent generally had clinical, laboratorial and histopathological features observed in patients with chronic HCV hepatitis and a high proportion could be identified in interviews and medical evaluation realized in blood blanks. Generally, these HCV infected donors are identified and discharged only by the serological tests results.Entre 1992 e 1997 foram avaliados, ambulatorialmente, 790 doadores de sangue com teste anti-HCV EIA-2 fortemente reagente (relação entre a densidade ótica da amostra / "cut-off" > 3, que haviam sido detectados na triagem sorológica do banco de sangue. Todos eram negativos para doença de Chagas, sífilis, hepatite B (HBsAg e AIDS. Amostras de sangue foram coletadas, na primeira consulta ambulatorial, para a realização de hemograma, exames bioquímicos e novos testes sorológicos para a HVC (anti-HCV EIA-2. Em 226 doadores anti-HCV EIA-2 repetidamente reagentes, realizou-se o teste suplementar de "immunoblot" para a HVC (RIBA-2. Em 209 doadores, pesquisou-se a presença do RNA do VHC pelo teste do PCR, através de exame automatizado (HCV-AMPLICOR, ROCHE. A ultra-sonografia abdominal foi realizada em 366 doadores e a biópsia hepática em 269 concordantes. Notou-se que 95,6% eram EIA-2 repetidamente reagentes, 94% eram assintomáticos e que apenas 2% referiram icterícia pregressa. Em 47% detectou-se, pelo menos, um fator de risco para a transmissão do VHC, sendo o uso de drogas E.V. o principal deles (27,8%. A transfusão de sangue foi o segundo fator na transmissão da HVC (27,2%. Hepatomegalia foi encontrada em 54%. Esplenomegalia e sinais de hipertens

  2. Espionage French in Algeria in the modern and contemporary era: spy León Roch الجوسسة الفرنسية في الجزائر في العصر الحديث والمعاصر: الجاسوس ليون روش

    Directory of Open Access Journals (Sweden)

    Djidel Aziz

    2010-12-01

    Full Text Available ليون روش الذي سنتناول جانباً من حياته في الجزائر قبل التحاقه بجيش الأمير (1832 - 1837 هو شخصية مغامرة يمتلك ذكاءً حادً وجرأة في نشاطه المستمر، ومما سهل له الأمر هو معرفته الجيدة للغة العربية التي تمكن من خلالها من فهم ذهنيات وعادات المسلمين، وأن يكون من ألمع الذين مروا على هيئة المترجمين العسكريين، والذين ساهموا في تقديم خدمات جليلة للجيش الإفريقي، وزيادة على تلك الخدمات التي قام بها في مجال الدبلوماسية في المغرب على أحسن وجه.

  3. Formation of Saturn's F ring by collision between rubble-pile satellites

    Science.gov (United States)

    Hyodo, Ryuki; Ohtsuki, Keiji

    2014-11-01

    Saturn’s F ring is located just outside the Roche limit. This pure icy ring is radially narrow and is thought to be dynamically young. Two shepherding satellites, inner Pandora and outer Prometheus, confine and regulate its current dynamical evolution. The bulk density of these satellites is lower than that of rigid water ice, thus they are likely to be rubble-pile bodies. Crida & Charnoz (2012) showed that Saturn’s inner major satellites are formed by spreading of ancient massive rings through the Roche limit using one-dimensional analytical model. Recently, we have performed N-body simulations of the evolution of circumplanetary particle disks initially confined within a planet’s Roche limit, and showed that rubble-pile co-orbital satellites are often formed just outside the Roche limit (Hyodo et al, submitted). However, these co-orbital satellites are not always stable but can experience collisions between them. In addition, at radial locations barely outside the Roche limit, accretion efficiency is not 100%, and collision between aggregates can lead to complete or partial disruption (Karjalainen 2007, Hyodo & Ohtsuki 2014).In the present work, we perform local N-body simulations in the Hill coordinate system and investigate collisional disruption of rubble-pule satellites just outside the Roche limit corresponding to the location of Saturn’s F ring. We find that in some cases, collision between two aggregates results in partial disruption such that the dispersed particles are distributed between the two remnant satellites with small radial extent. Our results suggest that the F ring is a relic of collisional disruption between rubble-pile satellites formed at the last stage of the formation of inner major satellites as the rings spread across the Roche limit.

  4. The Size of the Emitting Region in the Magnetic Eclipsing Cataclysmic Variable Stars

    CERN Document Server

    Andrych, Kateryna D

    2015-01-01

    We discuss a method for determination of the size of the emitting region close to the compact star in a binary system with eclipses by a secondary, which fills its Roche lobe. The often used approach is to model the Roche lobe by a sphere with the "effective radius" corresponding to the volume of the Roche lobe. This approach leads to a 4-6% overestimate of the radius, if taking into account the angular dimensions of the Roche lobe seen form the compact star. Andronov (1992) had shown that the projection of the Roche lobe onto the celestial sphere is close to an ellipse and had tabulated these dimensions as a function of the mass ratio. Also he published the coefficients of the approximation similar to that of the Eggleton (1983) for the "sphere corresponding to the same volume". We compare results obtained for the "circle+circle", "ellipse+circle" and "ellipse+point" approximations of the projections of the red dwarf and a white dwarf, respectively. Results are applied to the recently discovered eclipsing po...

  5. Viral blips during long-term treatment with standard or double dose lamivudine in HBe antigen negative chronic hepatitis B

    Institute of Scientific and Technical Information of China (English)

    2007-01-01

    AIM: To evaluate safety and effect on hepatitis B virus (HBV) suppression of a long-term treatment with lamivudine (LAM) at standard (100 mg/d) or double (200 mg/d) dose in chronic hepatitis B. METHODS: This was a case study with matched controls (1:3) in patients with chronic hepatitis B with anti-Hbe antibodies. RESULTS: Twelve patients received LAM 200 mg/d and 35 LAM 100 mg/d, for a median of 28 mo. A primary response (PR; I.e. Negative HBV-DNA with Amplicor assay) was achieved in 100% of LAM-200 patients and 83% of LAM-100 patients. A virological breakthrough occurred in 16.7 and 24.7%, respectively, of the PR-patients, with the appearance of typical LAM resistance mutations in all but one patient. Viremia blips (I.e. Transient HBV-DNA below 80 IU/Ml in patients who tested negative at Amplicor assay) were detected using a real time polymerase chain reaction (PCR) and occurred in seven out of nine patients with subsequent BT and in four out of 32 patients with end-of-study response (77.7% vs 12.5%; P = 0.001) at chi-square test). At the end of the study, 51.4% of LAM-100 patients and 83.3% of LAM-200 patients had remained stably HBV-DNA negative. Double-dose LAM was well tolerated. CONCLUSION: Long-term treatment of anti-Hbe positive chronic hepatitis B with double dose lamivudine causes a more profound and stable viral suppression as compared to conventional treatment.

  6. Effect of Antiviral Therapy on Serum Activity of Angiotensin Converting Enzyme in Patients with Chronic Hepatitis C

    Science.gov (United States)

    Husic-Selimovic, Azra; Sofic, Amela; Huskic, Jasminko; Bulja, Deniz

    2016-01-01

    Introduction: Renin-angiotenzin system (RAS) is frequently activated in patients with chronic liver disease. Angiotenzin - II (AT-II), produced by angiotenzin converting enzyme (ACE), has many physiological effects, including an important role in liver fibrogenesis. Combined antiviral therapy with PEG-IFN and ribavirin besides its antiviral effect also leads to a reduction in liver parenchyma fibrosis. Aim of the study: Determining the value of ACE in serum of patients with chronic hepatitis C before and after combined antiviral therapy, as well as the value of ACE activities in sera of the control group. Materials and methods: We studied 50 patients treated at Gastroenterohepatology Department, in the time-period of four years. Value of ACE in serum was determined by Olympus AU 400 device, with application of kit “Infinity TN ACE Liquid Stable Reagent”. HCV RNA levels in sera were measured by real time PCR. HCV RNA test was performed with modular analysis of AMPLICOR and COBAS AMPLICOR HCV MONITOR test v2.0, which has proved infection and was used for quantification of the viruses and monitoring of the patients’ response to therapy. Liver histology was evaluated in accordance with the level of necroinflammation activity and stage of fibrosis. Results: Serum activities of ACE in chronic hepatitis C patients is statistically higher than the values in the control group (p=0.02). Antiviral therapy in chronic hepatitis C patients statistically decreases serum activities of ACE (p= 0.02) and indirectly affects fibrogenesis of the liver parenchyma. Correlation between ACE and ALT activity after the therapy was proved (0.3934). Conclusion: Our findings suggest that the activity of ACE in serum is a good indirect parameter of the liver damage, and could be used as an indirect prognostic factor of the level of liver parenchyma damage. Serum activity of ACE can be used as a parameter for non-invasive assessment of intensity of liver damage. PMID:27147779

  7. Comparison of DNA extraction protocols for Mycobacterium Tuberculosis in diagnosis of tuberculous meningitis by real-time polymerase chain reaction

    Directory of Open Access Journals (Sweden)

    Rajeev Thakur

    2011-01-01

    Full Text Available Background: Several nucleic acid amplification techniques are available for detection of Mycobacterium tuberculosis (MTB in pulmonary and extrapulmonary samples, but insufficient data are available on the diagnostic utility of these techniques in tubercular meningitis where bacilli load is less. The success of final amplification and detection of nucleic acid depends on successful extraction of DNA from the organism. Aims: We performed this study to compare four methods of extraction of MTB DNA from cerebrospinal fluid (CSF samples so as to select one method of DNA extraction for amplification of nucleic acid from clinical samples. Materials and Methods: Four methods of extracting MTB DNA from CSF samples for testing by real-time polymerase chain reaction (PCR were compared: QIAGEN R protocol for DNA purification using QIAamp spin procedure (manual, AMPLICOR R respiratory specimen preparation kit, MagNA Pure R kit extraction, combined manual DNA extraction with automated extraction by MagNA Pure R . Real-time PCR was performed on COBAS TaqMan 48 Analyzer R with known positive and negative controls. Results: The detection limit for the combined manual and MagNA Pure R extraction protocol was found to be 100 copies of MTB DNA per reaction as against 1,000 copies of MTB DNA per reaction by the QIAGEN R , AMPLICOR R , and the MagNA Pure R extraction protocol. Conclusion: The real-time PCR assay employing the combination of manual extraction steps with MagNA Pure R extraction protocol for extraction of MTB DNA proved to be better than other extraction methods in analytical sensitivity, but could not detect less than 10 2 bacilli /ml.

  8. Duas novas espécies de Ptychoderes Schoenherr (Coleoptera, Anthribidae, Anthribinae, Ptychoderini Two new species of Ptychoderes Schoenherr (Coleoptera, Anthribidae, Anthribinae, Ptychoderini

    Directory of Open Access Journals (Sweden)

    José Ricardo M. Mermudes

    2004-03-01

    Full Text Available Duas novas espécies de Ptychoderes Schoenherr, 1823 são descritas e ilustradas: Ptychoderes magnus sp. nov., da Bolívia e Brasil (localidade-tipo: Ouro Preto d'Oeste, Rondônia e P. jekeli sp. nov. do Brasil e Guiana Francesa (localidade-tipo: Roches de Kourou.The following new species of Ptychoderes Schoenherr, 1823 are described and illustrated (type-localities parenthetic: Ptychoderes magnus sp. nov., from Bolivia and Brazil (Rondônia: Ouro Preto d'Oeste and P. jekeli sp. nov., from Brazil and French Guiana (Roches de Kourou.

  9. Multilaboratory Comparison of Hepatitis C Virus Viral Load Assays

    OpenAIRE

    Caliendo, A M; Valsamakis, A.; Zhou, Y.; Yen-Lieberman, B; Andersen, J; Young, S.; Ferreira-Gonzalez, A; Tsongalis, G. J.; Pyles, R.; Bremer, J W; Lurain, N. S.

    2006-01-01

    We report a multilaboratory evaluation of hepatitis C virus (HCV) viral load assays to determine their linear range, reproducibility, subtype detection, and agreement. A panel of HCV RNA samples ranging in nominal concentration from 1.0 to 7.0 log10 IU/ml was constructed by diluting a clinical specimen (genotype 1b). Replicates of the panel were tested in multiple laboratories using the Abbott TaqMan analyte-specific reagent (Abbott reverse transcription-PCR [RT-PCR]), Roche TaqMan RUO (Roche...

  10. [Leo Sternbach, an inventor of benzodiazepines].

    Science.gov (United States)

    Uchibayashi, Masao

    2007-01-01

    A biography of Leo Sternbach, an inventor of benzodiazepine tranquillizers, is presented. It consists of (1) a societal desire for lifestyle pills, (2) Leo's birth in 1908 and youth, (3) education, (4) in Vienna, (5) in Zurich, (6) at Hoffmann-La Roche, Basel, (7) to the New World, (8) at Roche, Nutley NJ, (9) invention of the new drugs, (10) revolution of people's lifestyle, and (11) reward, retirement and obituary in 2005. This paper may be the first comprehensive biography of this remarkable chemist written in Japanese.

  11. Model of contract of purchase of the electric power produced by facilities that use the energy of aquifers or of underground rocks and benefiting from the electricity purchase obligation. Established after enforcement of the article 5 of the decree from May 10, 2001 and approved by the minister attended to electricity; Modele de contrat d'achat de l'energie electrique produite par les installations utilisant l'energie des nappes aquiferes ou des roches souterraines et beneficiant de l'obligation d'achat d'electricite. Etablie en application de l'article 5 du decret du 10 mai 2001 et approuve par le Ministre charge de l'electricite

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2001-07-01

    This model of contract comprises 2 parts. The first part describes the general conditions of electric power purchase: aim of the contract, connection to the grid and delivery point, producer's facility, reciprocal commitments and stoppages for maintenance purpose, energy and power metering and control, energy delivery, payment for the purchased power (payment and payment indexing), taxes, payments, contract enforcement, date line, suspension, modification or cancellation, conciliation in case of dispute. A recall of the tariffs mentioned in the by-law from March 13, 2002, of the approximation rules and a model of certificate are given in appendixes. The second part gives some complements to the general conditions (purchaser and producer corporate, characteristics of the facility, details about the connection and delivery point, description of the metering system, tariffs of purchase and indexing, payment of bills, contract characteristics, subscription for a power supply contract). (J.S.)

  12. The Advantage of Reading over Listening Text Comprehension in Down Syndrome: What Is the Role of Verbal Memory?

    Science.gov (United States)

    Roch, Maja; Florit, Elena; Levorato, M. Chiara

    2012-01-01

    The current study was designed to investigate the role played by verbal memory in the advantage shown by individuals with Down syndrome in reading over listening text comprehension (Roch & Levorato, 2009). Two different aspects of verbal memory were analyzed: processing load and coding modality. Participants were 20 individuals with Down syndrome,…

  13. Reference intervals for thyreotropin and thyroid hormones for healthy adults based on the NOBIDA material and determined using a Modular E170

    DEFF Research Database (Denmark)

    Friis-Hansen, Lennart; Hilsted, Linda

    2008-01-01

    without medication. TSH, thyroxine, free thyroxine, triiodothyronine, free triiodothyronine, and thyroglobulin antibodies (Tg-ab) were measured using assays for Roche Modular E170 and thyroid peroxidase antibodies (TPO-ab) were measured using the Brahms Kryptor assay. RESULTS: The measured concentrations...

  14. Les Basaltes de l’Atlas de Béni-Mellal (Haut Atlas Central, Maroc : un Volcanisme Transitionnel Intraplaque Associé aux Stades de L’évolution Géodynamique du Domaine Atlasique

    Directory of Open Access Journals (Sweden)

    Jaouad Guezal

    2013-07-01

    Full Text Available Les roches basaltiques de l’Atlas de Béni-Mellal (Haut-Atlas Central, Maroc, mises en place, dans les différents bassins synclinaux de la région, se manifestent en deux horizons volcaniques B1 et B2. Les deux événements éruptifs sont d’âge Jurassique moyen pour les coulées de l’horizon B1 et d’âge Crétacé inférieur pour celles de l’horizon B2. Les roches sont caractérisées par un faciès des basaltes à olivines. La composition de leurs clinopyroxènes est marquée par deux tendances : augite au niveau des basaltes à olivine du B1 et diopside au niveau de ceux du B2. La composition géochimique sur roche totale dévoile le caractère transitionnel de ces roches. La relative dissimilitude géochimique signalée au niveau des basaltes des deux horizons peut être expliquée par un début du changement de la nature du magmatisme de la région en relation avec l’évolution géodynamique du domaine atlasique.

  15. Evaluation of a radioimmunoassay (/sup 125/I) kit for cannabinoid metabolites in urine and whole blood

    Energy Technology Data Exchange (ETDEWEB)

    Childs, P.S.; McCurdy, H.H.

    The Abuscreen kit (Roche Diagnostics) for the analysis of 11-nor-..delta../sup 9/-tetrahydrocannabinol-9-carboxylic acid and other cannabinoids in urine was evaluated in terms of its accuracy, reproducibility, and sensitivity. A procedure is also presented for the analysis of total cannabinoids in whole blood using the RIA kit.

  16. Sandy Point Fun Run: A Context for Understanding and Using Scale

    Science.gov (United States)

    Roche, Anne

    2013-01-01

    In the middle years of school, it is important that mathematics is challenging, engaging and focuses on worthwhile mathematics. In this article, Anne Roche describes a lesson that seemed to have all three of these characteristics, as students grappled with issues of scale to create a fun run, given a range of challenging mathematical constraints.…

  17. Performance of cobas® 4800 and m2000 real-time™ assays for detection of Chlamydia trachomatis and Neisseria gonorrhoeae in rectal and self-collected vaginal specimen

    NARCIS (Netherlands)

    Geelen, Tanja H; Rossen, John W; Beerens, Antoine M; Poort, Linda; Morré, Servaas A; Ritmeester, Wilma S; van Kruchten, Harry E; van de Pas, Masja M; Savelkoul, Paul H M

    2013-01-01

    A prospective, multicenter trial was designed to compare the performance characteristics of the cobas® 4800 (Roche Diagnostics, Indianapolis, IN, USA) and m2000 real-time™ (Abbott Molecular Inc., Des Plaines, IL, USA) assays for detection of Chlamydia trachomatis (CT) and Neisseria gonorrhoeae (NG)

  18. 78 FR 25259 - Notice of Intent To Grant an Exclusive License; Integrata Security, LLC

    Science.gov (United States)

    2013-04-30

    ... filed with the National Security Agency Technology Transfer Program, 9800 Savage Road, Suite 6848, Fort George G. Meade, MD 20755-6848. FOR FURTHER INFORMATION CONTACT: Marian T. Roche, Director, Technology Transfer Program, 9800 Savage Road, Suite 6848, Fort George G. Meade, MD 20755-6848, telephone (443)...

  19. Multiple nuclear ortholog next generation sequencing phylogeny of Daucus

    Science.gov (United States)

    Next generation sequencing is helping to solve the data insufficiency problem hindering well-resolved dominant gene phylogenies. We used Roche 454 technology to obtain DNA sequences from 93 nuclear orthologs, dispersed throughout all linkage groups of Daucus. Of these 93 orthologs, ten were designed...

  20. Validation of a norovirus multiplex real-time RT-PCR assay for the detection of norovirus GI and GII from faeces samples.

    LENUS (Irish Health Repository)

    Jones, S

    2011-01-01

    Norovirus is a leading cause of infectious non-bacterial gastroenteritis. The virus is highly contagious and has multiple modes of transmission, presenting a growing challenge to hospital-based healthcare. In this study, a total of 120 stool samples are tested for the presence of norovirus GI and GII by the Roche two-step Lightcycler 2.0 assay incorporating primers and probes produced by TIB Molbiol, and the results are compared with results from the National Virus Reference Laboratory. The Roche\\/TIB Molbiol assay produced 51 positive results and 69 negative results. Discrepancy analysis was performed for six conflicting results using a second real-time polymerase chain reaction (PCR) assay (Roche\\/TIB Molbiol) and this confirmed that four of the five discrepant positive results were true positives. A single discrepant negative result generated by the Roche assay remained negative using the second assay. Sensitivity, specificity, positive predictive value (PPV) and negative predictive value (NPV) were calculated to be 98%, 98.6%, 98.0% and 98.6%, respectively. Melting curve analysis was used to differentiate genogroups I and II and this showed that 92% of strains belonged to genogroup II.

  1. Fast and robust methods for full genome sequencing of Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) Type 1 and Type 2

    DEFF Research Database (Denmark)

    Kvisgaard, Lise Kirstine; Hjulsager, Charlotte Kristiane; Fahnøe, Ulrik;

    on primary material and cell culture adapted viruses and the protocols were shown to perform well on all three NGS platforms tested (Roche 454 FLX, Illumina HiSeq 2000, and Ion Torrent PGM™ Sequencer). To complete the sequences at the 5’ end, 5’ Rapid Amplification of cDNA Ends (5’ RACE) was conducted...

  2. Hypokalemia secondary to capecitabine: a hidden toxicity?

    OpenAIRE

    Saif, Muhammad Wasif; Fekrazad, Mohammad Houman; Ledbetter, Leslie; Diasio, Robert B

    2007-01-01

    Background Hyopkalemia is a listed toxicity in the capecitabine (Xeloda®; Roche, Nutley, NJ) package insert. However, the incidence and severity of this toxicity is not known. Methods We performed a retrospective evaluation of hypokalemia in 77 patients, who received capecitabine for gastrointestinal malignancies between April 2002 and November 2004. Hypokalemia was defined as K+ level

  3. Development of an oral dosage form of capecitabine with modified release characteristics

    NARCIS (Netherlands)

    Meulenaar, J

    2013-01-01

    Capecitabine is an orally-administered chemotherapeutic agent used in the treatment of a.o. metastatic breast, gastric and colorectal cancer and is commercially available as an immediate release tablet (Xeloda®, Roche). Capecitabine is a pre-pro-drug that is enzymatically converted into 5-fluorourac

  4. Nonlinear Time Series Analysis via Neural Networks

    Science.gov (United States)

    Volná, Eva; Janošek, Michal; Kocian, Václav; Kotyrba, Martin

    This article deals with a time series analysis based on neural networks in order to make an effective forex market [Moore and Roche, J. Int. Econ. 58, 387-411 (2002)] pattern recognition. Our goal is to find and recognize important patterns which repeatedly appear in the market history to adapt our trading system behaviour based on them.

  5. Formal synthesis of (+)-discodermolide.

    Science.gov (United States)

    Francavilla, Charles; Chen, Weichun; Kinder, Frederick R

    2003-04-17

    [structure: see text] Herein we report the formal total synthesis of (+)-discodermolide in 21 steps (longest linear sequence) from commercially available Roche ester. This synthesis features the assembly of C(9-18) and C(19-24) fragments via a metal-chelated aldol coupling reaction.

  6. A symplectic proof of Verlinde factorization

    OpenAIRE

    Meinrenken, Eckhard; Woodward, Chris

    1996-01-01

    We prove a multiplicity formula for Riemann-Roch numbers of reductions of Hamiltonian actions of loop groups. This includes as a special case the factorization formula for the quantum dimension of the moduli space of flat connections over a Riemann surface.

  7. Probing thermonuclear burning on accreting neutron stars

    NARCIS (Netherlands)

    Keek, L.

    2008-01-01

    Neutron stars are the most compact stars that can be directly observed, which makes them ideal laboratories to study physics at extreme densities. Neutron stars in low-mass X-ray binaries accrete hydrogen and helium from a lower-mass companion star through Roche lobe overflow. This matter undergoes

  8. [Examples of the keeping of drugs during the XVIth, XVIIth and XVIIIth centuries: silenes and drawers].

    Science.gov (United States)

    Fievet, B

    1998-01-01

    Silenes are wooden boxes with colourful paintings for the keeping of drugs and can be found nowadays in three collections in France: Hotel-Dieu in Troyes (Aube), a pharmacy in Bauge (Maine-et-Loire) and St-Roch museum in Issoudun (Indre). Drawers can be related to silenes but they are more ordinary and less attractive.

  9. Disease: H01221 [KEGG MEDICUS

    Lifescience Database Archive (English)

    Full Text Available the epithelium are the hallmarks of EBMD. Most patients are asymptomatic before ...roche L, Abitbol M, Schorderet DF A subset of patients with epithelial basement membrane corneal dystrophy have mutations in TGFBI/BIGH3. Hum Mutat 27:553-7 (2006) ...

  10. Writing to fellow physicians: literary genres and medical questions in Louis Odier’s (1748-1817) correspondence

    OpenAIRE

    Rieder, Philip Alexander

    2013-01-01

    Correspondance between physicians reveals much about their medical practices and the theoretical frame within which they evolve. Concentrating on a correspondance between Daniel De la Roche and Louis Odier, this chapter explores the uses these authors make of case histories and the different cultural genres to which they refer.

  11. Last Rites for Cataclysmic Variables: Death by Fire, or Ice? (Abstract)

    Science.gov (United States)

    Patterson, J.

    2016-06-01

    (Abstract only) Cataclysmic binaries lose angular momentum as they age, and thus the component stars in the binary spiral ever closer together. As the spiraling-in proceeds, the shrinking Roche lobe "strangles" the donor star, forcing it to transfer mass. Since we can measure the rate of mass transfer pretty well, we know the rate of angular momentum loss pretty well.

  12. A superconformal algebra of meromorphic vector fields with three poles on the super Riemann sphere

    International Nuclear Information System (INIS)

    Based on the Riemann-Roch theorem, we construct a superconformal algebra of meromorphic vector fields with three poles and the relevant abelian differential of the third kind on the super Riemann sphere. The algebra includes two Ramond sectors as a subalgebra, and implies the picture of an interaction of three superstrings. (orig.)

  13. Two new species of Metrosideros (Myrtaceae) from New Caledonia: dual characterisation with morphology and nrDNA sequence variation

    NARCIS (Netherlands)

    Dawson, John W.; Whitaker, Anthony H.; Whitaker, Vivienne A.; Gardner, Richard C.; Wright, Shane D.

    2000-01-01

    We have identified two new species of Metrosideros subg. Mearnsia (Myrtaceae) growing on, and apparently endemic to, the massif known as the Roches d’Ouaïème in New Caledonia. Of these two species, we include M. whitakeri in sect. Mearnsia and M. rotundifolia in sect. Calyptropelala. Both morphologi

  14. Evaluation of NGAL TestTM on Cobas 6000

    DEFF Research Database (Denmark)

    Hansen, Young B L; Damgaard, Anette; Poulsen, Jørgen H

    2014-01-01

    BACKGROUND: Neutrophil Gelatinase-Associated Lipocalin (NGAL) is a promising biomarker for acute kidney injury (AKI). Our objectives were to evaluate the NGAL Test(TM) from Bioporto for both urine NGAL and plasma NGAL on the Cobas 6000 c501 (Roche Diagnostics, Rotkreuz, Switzerland) with matched ...

  15. The American cranberry mitochondrial genome reveals the presence of selenocysteine (tRNA-Sec and SECIS) insertion machinery in land plants

    Science.gov (United States)

    The American cranberry (Vaccinium macrocarpon Ait.) mitochondrial genome was assembled and reconstructed from whole genome 454 Roche GS-FLX and Illumina shotgun sequences. Compared with other Asterids, the reconstruction of the genome revealed an average size mitochondrion (459,678 nt) with comparat...

  16. 电化学发光法联合检测HIV抗原抗体试剂的评价%The evaluation of Elecsys HIV Combi reagent in detecting HIV antigen and antibody

    Institute of Scientific and Technical Information of China (English)

    周琰; 宋斌斌; 郑晓虹; 郭玮; 潘柏申

    2011-01-01

    目的 评价Roche电化学发光法联合检测HIV抗原抗体试剂的检测性能.方法 收集包括健康献血员、人类免疫缺陷病毒( HIV)感染者、丙型肝炎病毒(HCV)抗体阳性和高危人群在内的血清样本1 327例.购买美国BBI公司的7套HIV血清转换盘共计51份血清样本.采用Roche cobas e411(架式系统)电化学全自动免疫分析仪和相应的检测试剂、校准品和质控品以及检测参数进行测定.以Roche Elecsys HIV Combi试剂盒为评估试剂,以生物梅里埃Vironostika Uni-FormⅡplus O HIV抗体试剂盒为参比试剂.定性检测血清HIV p24抗原和HIVl/2抗体并评价检测方法分析性能、结果一致性和血清转化灵敏度.结果 Roche Elecsys HIV Combi试剂盒批内和天间不精密度良好,符合临床使用要求.血清样本检测结果和参比试剂检测结果一致性完全符合.评估试剂的血清转化灵敏度比参比试剂平均提前了5.1d.结论 Roche Elecsys HIV Combi试剂盒具有良好的精密度和血清转换灵敏度,有利于筛查HIV早期感染,能为临床HIV早期诊断、随访监测提供有效的实验室诊断依据.%Objective To evaluate the performance of Roche Elecsys HIV Combi reagent in detecting HIV p24antigen and HIV1/2 antibody. Methods A total of 1 327 serum samples were collected for healthy subjects, patients with human immunodeficiency virus (HIV) infection and patients with antibody positive and high-risk hepatitis C virus ( HCV). The 1 327 serum samples were detected by Roche cobas e411 Elecsys HIV Combi reagent and Vironostika UniFormll plus 0 HIV reagent, respectively. The both reagents were used to detect 51 serum samples of 7 seroconversion panels from American BBI company, and the analytical performance, consistency and the sensitivity of seroconversion were evaluated. Roche Elecsys HIV Combi reagent was as evaluation reagent, and Vironostika Uni-Form II plus 0 HIV reagent was as reference reagent. Results The Roche Elecsys

  17. Prevalence of Chlamydia trachomatis in women attending sexually transmitted disease clinics in the Colombo district, Sri Lanka

    Directory of Open Access Journals (Sweden)

    Gunasekera Henadira Appuhamilage Kamani Mangalika

    2014-01-01

    Full Text Available Background: In Sri Lanka little is known about the prevalence of Chlamydia trachomatis (CT infection. Objective was to determine the prevalence of CT in female patients attending sexually transmitted disease (STD clinics in the Colombo district. Materials and Methods: A descriptive cross-sectional study was carried out for the prevalence of CT in all female patients (n = 168 more than 18 years of age, attending two STD clinics in the Colombo district from January to May 2012. Endocervical swabs were collected and tested for CT using the Amplicor CT/NG polymerase chain reaction assay. Results: Prevalence of CT in females attending the STD clinics in the Colombo district was 8.3%. Mean age of those infected with CT was 32.9 years (SD ± 8.2. Majority of females with CT infections were Sinhalese and married. There was no significant association with age, ethnicity or being married or not. Females who did not attend school, or had their education only up to Grade 5 were significantly found to have six times the risk of having CT infection (95% CI = 1.8-22.6. A significant association was found with number of sexual partners but not with commercial sex work or past history of STD. Conclusions: Prevalence of CT was moderately high in this population.

  18. Factors influencing a low rate of hepatitis C viral RNA clearance in heroin users from Southern China

    Institute of Scientific and Technical Information of China (English)

    ShengHan Lai; Jin-Bing Zhang; Wei Liu; Jie Chen; Xiao-Fang Yu

    2008-01-01

    AIM: To study the virological and host factors influencing hepatitis C infection outcomes in heroin users in southern China.METHODS: HCV RNA and associated factors were analyzed among 347 heroin users from Guangxi Zhuang Autonomous Region, southern China who were hepatitis C virus (HCV) EIA positive for two or more consecutive visits.RESULTS: Using the COBAS AMPLICOR HCV TEST, a remarkably low HCV RNA negative rate of 8.6% was detected. After multivariate logistic regression analysis, HCV RNA clearance was significantly associated with the presence of HBsAg (OR = 8.436, P < 0.0001), the lack of HIV-1 infection (OR = 0.256, P = 0.038) and age younger than 25 (OR = 0.400, P = 0.029).CONCLUSION: Our study suggests HCV infection among Chinese heroin users results in high levels of viral persistence even amidst factors previously found to enhance viral clearance. Prospective studies of a possible genetic component within the Chinese population and the pathogenicity of non-genotype 1 HCV infections are needed.

  19. The impact of polymerase chain reaction assays for the detection of hepatitis C virus infection in a hemodialysis unit.

    Science.gov (United States)

    Hussein, Magdi M; Mooij, Jaap M; Hegazy, Mohamed S; Bamaga, Mohammed S

    2007-03-01

    Hepatitis C virus (HCV) infection is most often diagnosed by detection of antibodies against the virus (HCV Ab). However, it has been reported that some HCV Ab negative patients test positive for HCV-RNA. Over a study period of 30 months, all patients on hemodialysis at the Al Hada Armed Forces Hospital in Taif, Saudi Arabia were tested monthly for HCV Ab and twice per year for HCV-RNA. HCV Ab was tested by a third generation microparticle enzyme immunoassay (MEIA), and HCV-RNA by a qualitative hepatitis-RNA assay, second version (COBAS Amplicor PCR), which was recently introduced in the Molecular Pathology Laboratory of our hospital. Of the 180 patients studied, 34 (18.9%) had positive HCV Ab, and of the 146 HCV Ab negative patients, five patients tested positive for HCV-RNA (3.42%). Our study further finds that, when applying HCV Ab testing only, some patients with HCV viremia may be undetected. For better HCV infection control, routine HCV-RNA testing of dialysis patients should be considered, particularly in areas where the infection is common and in units applying isolation policies.

  20. [Clinical benefit of HCV core antigen assay in patients receiving interferon and ribavirin combination therapy].

    Science.gov (United States)

    Higashimoto, Makiko; Takahashi, Masahiko; Jokyu, Ritsuko; Saito, Hidetsugu

    2006-02-01

    A highly sensitive second generation HCV core antigen assay has recently been developed. We compared viral disappearance and kinetics data between commercially available core antigen assays, Lumipulse Ortho HCV Ag, and a quantitative HCV RNA PCR assay, Cobas Amplicor HCV Monitor Test, Version 2 to estimate the predictive benefit of sustained viral response (SVR) and non-SVR in 59 patients treated with interferon and ribavirin combination therapy. We found a good correlation between HCV core Ag and HCV RNA level regardless of genotype. Although the sensitivity of the core antigen assay was lower than PCR, the dynamic range was broader than that of the PCR assay, so that we did not need to dilute the samples in 59 patients. We detected serial decline of core Ag levels in 24 hrs, 7 days and 14 days after interferon combination therapy. The decline of core antigen levels was significant in SVR patients compared to non-SVR as well as in genotype 2a, 2b patients compared to 1b. Core antigen-negative on day 1 could predict all 10 SVR patients (PPV = 100%), whereas RNA-negative could predict 22 SVR out of 25 on day 14 (PPV = 88.0%). None of the patients who had detectable serum core antigen on day 14 became SVR(NPV = 100%), although NPV was 91.2% on RNA negativity. An easy, simple, low cost new HCV core antigen detecting system seems to be useful for assessing and monitoring IFN treatment for HCV.

  1. Prevalence of Hepatitis C Virus in the Population of Albania for the Period 2007-2010

    Directory of Open Access Journals (Sweden)

    Hysaj Vila Brunilda

    2014-09-01

    Full Text Available BACKGROUND: Hepatitis C is a blood-borne, infectious, viral disease that is caused by a hepatotropic virus called Hepatitis C virus (HCV. AIM: The aim of this study is to determine the prevalence of active HCV infection (HCV–RNA in the cases that were anti-HCV positive. MATERIAL AND METHODS: Plasma of 301 high-risk for HCV infection consecutive from University Hospital Centre “Mother Theresa” Tirana-Albania, during January 2007 to December 2010 was included in this study. To identify the presence of HCV RNA, the samples were examined by Cobas Amplicor HCV test (qualitative method. RESULTS: From 301 samples analyzed in total, 214 of them resulted positive for the presence of HCV-RNA's, corresponding to a prevalence of 71.1%, with 95% CI interval [65.8 - 75.9] for value of χ2 = 52.7 p value 25 years with a significant difference with other age groups for p value 25 years.

  2. Analysis of HCV genotypes from blood donors shows three new HCV type 6 subgroups exist in Myanmar.

    Directory of Open Access Journals (Sweden)

    Shinji T

    2004-06-01

    Full Text Available The prevalence of hepatitis C virus (HCV genotypes in Myanmar in comparison with the rest of Southeast Asia is not well known. Serum samples were obtained from 201 HCV antibody-positive volunteer blood donors in and around the Myanmar city of Yangon. Of these, the antibody titers of 101 samples were checked by serial dilution using HCV antibody PA test II and Terasaki microplate as a low-cost method. To compare antibody titers by this method and RNA identification, we also checked HCV-RNA using the Amplicor 2.0 test. Most high-titer groups were positive for HCV-RNA. Of the 201 samples, 110 were successfully polymerase chain reaction (PCR amplified. Among them, 35 (31.8% were of genotype 1, 52 (47.3% were of genotype 3, and 23 (20.9% were of type 6 variants, and phylogenetic analysis of these type 6 variants revealed that 3 new type 6 subgroups exist in Myanmar. We named the subgroups M6-1, M6-2, and M6-3. M6-1 and M6-2 were relatively close to types 8 and 9, respectively. M6-3, though only found in one sample, was a brand-new subgroup. These subtypes were not seen in Vietnam, where type 6 group variants are widely spread. These findings may be useful for analyzing how and when these subgroups were formed.

  3. Early Biochemical Screening for Fetal Aneuploidy in the First Trimester

    DEFF Research Database (Denmark)

    Tørring, Niels

    2013-01-01

    validation of the Roche Elecsys® free βhCG and PAPP-A assays were performed, including method comparisons with the Brahms Kryptor, AutoDELFIA PerkinElmer and Siemens Immulite assays. Furthermore a clinical validation including genertion of assay specific medians for PAPP-A and free βhCG from gestational age...... 8+0 to 14+0 weeks, and clinical test performance of risk assesment was conducted. Results The in-between day imprecision of the Elecsys® free βhCG and PAPP-A assays were between 1.0 and 2.8%. Comparison (Passing/Bablok regression) of free βhCG and PAPP-A from Roche Elecsys® and the Brahms Kryptor...

  4. Tidal Forces in Naked Singularity Backgrounds

    CERN Document Server

    Goel, Akash; Roy, Pratim; Sarkar, Tapobrata

    2015-01-01

    The end stage of a gravitational collapse process can generically result in a black hole or a naked singularity. Here we undertake a comparative analysis of the nature of tidal forces in these backgrounds. The effect of such forces is generically exemplified by the Roche limit, which predicts the distance within which a celestial object disintegrates due to the tidal effects of a second more massive object. In this paper, using Fermi normal coordinates, we numerically compute the Roche limit for a class of non-rotating naked singularity backgrounds, and compare them with known results for Schwarzschild black holes. Our analysis indicates that there might be substantially large deviations in the magnitudes of tidal forces in naked singularity backgrounds, compared to the black hole cases. If observationally established, these can prove to be an effective indicator of the nature of the singularity at a galactic centre.

  5. NP Pavonis : Differential Corrections Analysis of the UBV Observations

    Directory of Open Access Journals (Sweden)

    M. A. Cerruti

    2003-01-01

    Full Text Available Se presenta el primer análisis fotoeléctrico de la binaria NP Pav basado en curvas de luz U, B y V y modelado usando el tratamiento de Wilson & Devinney. Las curvas están definidas por 3861 observaciones individuales. Las componentes y la orbita parecen, en general, ser estables. La primaria, más masiva y más luminosa, es la componente de mayor tamaño y está eclipsada en el mínimo primario y no llena su lóbulo de Roche (94%. La secundaria prácticamente llena su lóbulo de Roche (99% y tiene una diferencia de temperatura de =1330oK. Los elementos absolutos se obtuvieron suponiendo que la primaria sigue la relación masa-luminosidad.

  6. Le grès ferrugineux de Puisaye : provenance et utilisation architecturale

    Directory of Open Access Journals (Sweden)

    Lucile Subtil

    2008-09-01

    Full Text Available L’objectif de cette étude était de définir le faciès du grès ferrugineux de Puisaye ainsi que ses zones d’exploitation, puis de déterminer l’utilisation architecturale de cette roche dans les édifices monumentaux (XIe au XVIe siècle et d’en définir les aires de diffusion.Cette roche est issue d’intercalations gréseuses et locales à l’intérieur de l’importante formation détritique de l’Albien (Crétacé inférieur. Cet ensemble forme une écharpe, d’orientation sud-ouest - nord-est entre l’Yonne...

  7. High-throughput sequencing offers insight into mechanisms of resource partitioning in cryptic bat species

    DEFF Research Database (Denmark)

    Razgour, Orly; Clare, Elizabeth L.; Zeale, Matt R.K.;

    2011-01-01

    cryptic bat species that are sympatric in southern England (Plecotus austriacus and P. auritus) (Fig. 1). Using Roche FLX 454 (Roche, Basel, CH) high-throughput sequencing (HTS) and uniquely tagged generic arthropod primers, we identified 142 prey Molecular Operational Taxonomic Units (MOTUs) in the diet...... of the cryptic bats, 60% of which were assigned to a likely species or genus. The findings from the molecular study supported the results of microscopic analyses in showing that the diets of both species were dominated by lepidopterans. However, HTS provided a sufficiently high resolution of prey...... identification to determine fine-scale differences in resource use. Although both bat species appeared to have a generalist diet, eared-moths from the family Noctuidae were the main prey consumed. Interspecific niche overlap was greater than expected by chance (O(jk) = 0.72, P <0.001) due to overlap in the...

  8. Contraction of the solar nebula

    Energy Technology Data Exchange (ETDEWEB)

    Rawal, J.J. (Nehru Planetarium, Bombay (India))

    1984-10-01

    The concept of Roche limit is applied to the Laplacian theory of the origin of the solar system to study the contraction of a spherical gas cloud (solar nebula). In the process of contraction of the solar nebula, it is assumed that the phenomenon of supersonic turbulent convection is operative and brings about the halt at various stages of contraction. It is found that the radius of the contracting solar nebula follows the Titius-Bode law. The consequences of the relation are also discussed. The aim is to attempt to explain, on the basis of the concept of Roche limit, the distribution of planets in the solar system and try to understand the physics underlying it.

  9. Identification of novel viruses using VirusHunter--an automated data analysis pipeline.

    Directory of Open Access Journals (Sweden)

    Guoyan Zhao

    Full Text Available Quick and accurate identification of microbial pathogens is essential for both diagnosis and response to emerging infectious diseases. The advent of next-generation sequencing technology offers an unprecedented platform for rapid sequencing-based identification of novel viruses. We have developed a customized bioinformatics data analysis pipeline, VirusHunter, for the analysis of Roche/454 and other long read Next generation sequencing platform data. To illustrate the utility of VirusHunter, we performed Roche/454 GS FLX titanium sequencing on two unclassified virus isolates from the World Reference Center for Emerging Viruses and Arboviruses (WRCEVA. VirusHunter identified sequences derived from a novel bunyavirus and a novel reovirus in the two samples respectively. Further sequence analysis demonstrated that the viruses were novel members of the Phlebovirus and Orbivirus genera. Both Phlebovirus and Orbivirus genera include many economic important viruses or serious human pathogens.

  10. Le corps igné de Nefza (Tunisie septentrionale) : caractéristiques géophysiques et discussion du mécanisme de sa mise en place

    OpenAIRE

    Jallouli, C.; Inoubli, M. H.; Albouy, Yves

    1996-01-01

    La présence de roches ignées a été mise en évidence dans le Nord-Ouest de la Tunisie dans les régions de Nefza et des Mogod. La répartition géographique de ces indices montre qu'ils sont alignés suivant la direction Est-Ouest (N80°). L'interprétation des données aéromagnétiques et gravimétriques, conjuguée avec une modélisation géophysique du type 2D 1/2 ont permis de caractériser le corps igné de la région de Nefza. Les résultats montrent qu'il s'agit d'une roche intra-sédimentaire de densit...

  11. WASP-103b: a new planet at the edge of tidal disruption

    CERN Document Server

    Gillon, M; Collier-Cameron, A; Delrez, L; Hellier, C; Jehin, E; Lendl, M; Maxted, P F L; Pepe, F; Pollacco, D; Queloz, D; Segransan, D; Smith, A M S; Smalley, B; Southworth, J; Triaud, A H M J; Udry, S; Van Grootel, V; West, R G

    2014-01-01

    We report the discovery of WASP-103b, a new ultra-short-period planet (P=22.2 hr) transiting a 12.1 V-magnitude F8-type main-sequence star (1.22+-0.04 Msun, 1.44-0.03+0.05 Rsun, Teff = 6110+-160 K). WASP-103b is significantly more massive (1.49+-0.09 Mjup) and larger (1.53-0.07+0.05 Rjup) than Jupiter. Its large size and extreme irradiation (around 9 10^9 erg/s/cm^2) make it an exquisite target for a thorough atmospheric characterization with existing facilities. Furthermore, its orbital distance is less than 20% larger than its Roche radius, meaning that it might be significantly distorted by tides and might experience mass loss through Roche-lobe overflow. It thus represents a new key object for understanding the last stage of the tidal evolution of hot Jupiters.

  12. Le Mésolithique du Morbihan intérieur : bilan documentaire et problématique

    OpenAIRE

    Barracand, Gaël

    2014-01-01

    Des prospections réalisées dans la commune de Bieuzy ont permis de mettre au jour une collection composée de vingt types de roches dont l’ultramylonite dite de Tréméven et le grès éocène. Certaines roches semblent être originaires d’au moins soixante kilomètres du site. L’éloignement de la côte explique le pourcentage réduit de silex. Les vestiges lithiques de Lande Kerroc’h témoignent d’adaptations locales du débitage en fonction des ressources pétrographiques. Ce type de site découvert en p...

  13. Characteristic classes of flags of foliations and Lie algebra cohomology

    OpenAIRE

    Khoroshkin, Anton

    2013-01-01

    We prove the conjecture by Feigin, Fuchs and Gelfand describing the Lie algebra cohomology of formal vector fields on an $n$-dimensional space with coefficients in symmetric powers of the coadjoint representation. We also compute the cohomology of the Lie algebra of formal vector fields that preserve a given flag at the origin. The latter encodes characteristic classes of flags of foliations and was used in the formulation of the local Riemann-Roch Theorem by Feigin and Tsygan. Feigin, Fuchs ...

  14. Nonlinear Dynamics, Lorenz Model and Formation of Binary Stars

    OpenAIRE

    Chang, Yi-Fang

    2008-01-01

    Based on the Lorenz model derived from the equations of hydrodynamics of nebula, we discuss the formation of binary stars by the qualitative analysis theory of nonlinear equation. Here the two wings in the Lorenz model form just the binary stars, whose Roche surface is result of evolution under certain condition. The nonlinear interaction plays a crucial role, and is necessary condition of the formation of binary stars and of multiple stars. While the linear equations form only a single star....

  15. Bilingualism in Song: The Rabbit Song of the Fulaan Nara Huzhu Mongghul

    OpenAIRE

    Qi Huimin; Burgel RM Levy

    2015-01-01

    Qi Huimin and Burgel RM Levy. 2015. Bilingualism in Song: The Rabbit Song of the Fulaan Nara Huzhu Mongghul IN Gerald Roche and CK Stuart (eds) Asian Highlands Perspectives 36: Mapping the Monguor, 106-113, 301-332. Musical notation, and musical and linguistic characteristics of a Chinese-Huzhu Mongghul bilingual song in the Fulaan Nara dialect of Huzhu Mongghul are given. Huzhu Mongghul is an endangered language of the Monguor (Tu) subgroup of the Mongolic language family, spoken in th...

  16. Introductory note on Emergent Unconscious Knowledge Networks (Asygnodic Networks)

    OpenAIRE

    Edward M. Roche; Blaine, Michael J.; Bakis, Henry

    2016-01-01

    This note introduces the following paper on the concept of Emergent Unconscious Knowledge Networks (Asygnodic Networks) created by E. Roche and M. Blaine. The concept of asyngnosis explains a large number of diverse phenomena involving organizations, groups and decision making. It will present the genesis and definition of Asygnodic Networks and will focus on how they raise challenges to traditional theories of decision making and emerging social networks.

  17. The market in second-hand clothes and furnishings in Venice, c1500-c1650

    OpenAIRE

    ALLERSTON, Patricia Anne

    1996-01-01

    Defence date: 7 June 1996 Examining board: Prof. Franco Angiolini, Università degli Studi di Pisa (supervisor) ; Prof. Laurence Fontaine, European University Institute and CNRS, Paris ; Dr. Richard Mackenney, University of Edinburgh (external supervisor) ; Prof. Paolo Malanima, Università degli Studi di Pisa ; Prof. Daniel Roche, Institut d'Histoire Moderne et Contemporaine, CNRS, Paris First made available online on 10 September 2013. The object of this study is to reinstate the mar...

  18. The Waddlia genome: a window into chlamydial biology

    OpenAIRE

    C. Bertelli; Collyn, F.; Croxatto, A; Rückert, C; Polkinghorne, A; Kebbi-Beghdadi, C; Goesmann, A; Vaughan, L.; Greub, G

    2010-01-01

    Growing evidence suggests that a novel member of the Chlamydiales order, Waddlia chondrophila, is a potential agent of miscarriage in humans and abortion in ruminants. Due to the lack of genetic tools to manipulate chlamydia, genomic analysis is proving to be the most incisive tool in stimulating investigations into the biology of these obligate intracellular bacteria. 454/Roche and Solexa/Illumina technologies were thus used to sequence and assemble de novo the full genome of the first repre...

  19. The Waddlia genome: a window into chlamydial biology.

    OpenAIRE

    Claire Bertelli; François Collyn; Antony Croxatto; Christian Rückert; Adam Polkinghorne; Carole Kebbi-Beghdadi; Alexander Goesmann; Lloyd Vaughan; Gilbert Greub

    2010-01-01

    Growing evidence suggests that a novel member of the Chlamydiales order, Waddlia chondrophila, is a potential agent of miscarriage in humans and abortion in ruminants. Due to the lack of genetic tools to manipulate chlamydia, genomic analysis is proving to be the most incisive tool in stimulating investigations into the biology of these obligate intracellular bacteria. 454/Roche and Solexa/Illumina technologies were thus used to sequence and assemble de novo the full genome of the first repre...

  20. Evaluation of Linear Array Human Papillomavirus Genotyping Using Automatic Optical Imaging Software▿

    OpenAIRE

    Jeronimo, J; Wentzensen, N; R. Long; Schiffman, M; Dunn, S. T.; Allen, R A; Walker, J L; Gold, M.A.; Zuna, R. E.; Sherman, M E; Wacholder, S.; Wang, S S

    2008-01-01

    Variations in biological behavior suggest that each carcinogenic human papillomavirus (HPV) type should be considered individually in etiologic studies. HPV genotyping assays might have clinical applications if they are approved for use by the FDA. A widely used genotyping assay is the Roche Linear Array HPV genotyping test (LA). We used LA to genotype the HPV isolates from cervical specimens from women with the full spectrum of cervical disease: cervical cancer, cervical intraepithelial neop...

  1. Adding particle collisions to the formation of asteroids and Kuiper belt objects via streaming instabilities

    OpenAIRE

    Johansen, Anders; Youdin, A. N.; Lithwick, Y.

    2012-01-01

    Modelling the formation of super-km-sized planetesimals by gravitational collapse of regions overdense in small particles requires numerical algorithms capable of handling simultaneously hydrodynamics, particle dynamics and particle collisions. While the initial phases of radial contraction are dictated by drag forces and gravity, particle collisions become gradually more significant as filaments contract beyond Roche density. Here we present a new numerical algorithm for treating momentum an...

  2. High-precision photometry by telescope defocusing – VII. The ultrashort period planet WASP-103

    DEFF Research Database (Denmark)

    Southworth, John; Mancini, L.; Ciceri, S.;

    2015-01-01

    for these calculations. The planet has a Roche lobe filling factor of 0.58, leading to a significant asphericity; we correct its measured mass and mean density for this phenomenon. A high-resolution Lucky Imaging observation shows no evidence for faint stars close enough to contaminate the point spread function of WASP...... mass which is too small by a factor of five, implying that Rayleigh scattering is not the main cause of the variation of radius with wavelength....

  3. Leadership skills for nursing unit managers to decrease intention to leave

    OpenAIRE

    Roche MA; Duffield C; Dimitrelis S; Frew B

    2015-01-01

    Michael A Roche,1 Christine Duffield,1,2 Sofia Dimitrelis,1 Belinda Frew1 1Centre for Health Services Management, Faculty of Health, University of Technology, Sydney, NSW, 2Clinical Nursing and Midwifery Research Centre, School of Nursing and Midwifery, Edith Cowan University, Joondalup, WA, Australia Aim: To examine specific elements of nursing leadership linked to intention to leave, in public acute care hospitals. Background: Nurse turnover is a global issue receiving widespread attention...

  4. Use of capecitabine in management of early colon cancer

    OpenAIRE

    Cassidy J; Hameed H

    2011-01-01

    H Hameed, J CassidyBeatson West of Scotland Cancer Centre, Glasgow, Scotland, UKAbstract: Capecitabine (Xeloda®, Roche, Basel, Switzerland) is a pro-drug of 5-fluorouracil (5-FU), and it is converted to 5-FU in the cancer cell by enzymatic degradation. The role of capecitabine in colorectal cancer has evolved in the last 15 years. In early trials in the metastatic setting, capecitabine has shown superior response rates compared with those achieved with 5-FU (Mayo Clinic regimen) (26% ...

  5. NICE批准Xeloda用于胃癌

    Institute of Scientific and Technical Information of China (English)

    2010-01-01

    英国卫生费用管理机构(NICE)称,它推荐Roche Holding AG(RHHBY)公司上市的化疗药Xeloda(capecitabine,卡培他滨)(Ⅰ)作为公费药与基于铂剂(Ⅱ)的治疗方案联用,用于不能手术的晚期胃癌的一线治疗。

  6. Note sur un Lepidocyclina nouveau de Bornéo

    NARCIS (Netherlands)

    Schlumberger, C.

    1899-01-01

    Les travaux des géologues hollandais et de quelques savants anglais nous ont déjà fait connaître un bon nombre de foraminifères fossiles de Java et de Borneo, mais il reste encore à glaner après eux. Je viens de trouver dans une roche calcaire de Borneo que Mons. MARTIN, professeur à Leiden, a bien

  7. Note sur deux espèces de Lepidocyclina des Indes Néerlandaises

    NARCIS (Netherlands)

    Schlumberger, C.

    1899-01-01

    Je dois à l’obligeance de Monsieur K. MARTIN professeur de Géologie à l’Université de Leiden l’envoi pour ma collection de foraminifères de quelques doubles des roches rapportées de Java par Monsieur VERBEEK et j’ai eu la bonne fortune d’y rencontrer deux espèces d’Orbitoïdes du sous-genre Lepidocyc

  8. First Isolation of a Giant Virus from Wild Hirudo medicinalis Leech: Mimiviridae isolation in Hirudo medicinalis

    OpenAIRE

    Mondher Boughalmi; Isabelle Pagnier; Sarah Aherfi; Philippe Colson; Didier Raoult; Bernard La Scola

    2013-01-01

    Giant viruses and amoebae are common in freshwater, where they can coexist with other living multicellular organisms. We screened leeches from the species Hirudo medicinalis for giant viruses. We analyzed five H. medicinalis obtained from Tunisia (3) and France (2). The leeches were decontaminated and then dissected to remove internal parts for co-culture with Acanthamoeba polyphaga. The genomes of isolated viruses were sequenced on a 454 Roche instrument, and a comparative genomics analysis ...

  9. What innovative business models can be triggered by precision medicine? Analogical reasoning from the magazine industry

    OpenAIRE

    Sabatier, Valérie

    2015-01-01

    Neva Bojovic,1 Valérie Sabatier,1 Stephane Rouault2 1Grenoble Ecole de Management, Grenoble, France; 2Innovation Hub, Strategic Innovation, F Hoffmann-La Roche Ltd, Basel, Switzerland Abstract: Presently, the health care industry is facing many technological and organizational challenges, and the emergence of precision medicine is bringing innovation and potentially a complete redefinition of the industry. This study suggests several paths for incumbent biopharmaceutical companies...

  10. Lava caves of the Republic of Mauritius, Indian Ocean

    OpenAIRE

    Gregory J. Middleton

    1998-01-01

    In their Underground Atlas, MIDDLETON & WALTHAM (1986) dismissed Mauritius as: “very old volcanic islands with no speleological interest”. Recent investigations indicate this judgement is inaccurate; there are over 50 significant caves, including lava tube caves up to 687 m long (one 665 m long was surveyed as early as 1769) and 35 m wide. Plaine des Roches contains the most extensive system of lava tube caves with underground drainage rising at the seashore. Notable fauna includes an insecti...

  11. SUCCESSION PLANNING: EVIDENCE FROM BEST COMPANIES IN NEW YORK

    OpenAIRE

    Susan J. Kowalewski; Linda Moretti; Denny McGee

    2011-01-01

    Succession planning, as defined by Butler and Roche-Tarry (2002), is an ongoing dynamic process that assists a business or organization in aligning its goals and its human capital needs. Succession planning can prepare companies for future critical vacancies of middle and senior management positions. Forecasting the talent necessary for these roles can provide the groundwork and understanding of the characteristics needed to maintain and grow a company’s strategic plan. Both short and long-...

  12. Cytokeratin 19-fragments (CYFRA 21-1) as a novel serum biomarker for response and survival in patients with advanced pancreatic cancer

    OpenAIRE

    Boeck, S.; Wittwer, C; Heinemann, V; Haas, M.; Kern, C; Stieber, P; Nagel, D; Holdenrieder, S

    2013-01-01

    Background: CYFRA 21-1 serves as biomarker in several epithelial malignancies. However, its role in pancreatic cancer (PC) has not yet been investigated. Methods: Within a prospective single-centre study serial blood samples were collected from patients with confirmed advanced PC. Pre-treatment values and weekly measurements of CYFRA 21-1, carbohydrate antigen 19-9 (CA 19-9) and carcinoembryonic antigen (assessed by Elecsys 2010, Roche Diagnostics) during palliative first-line chemotherapy we...

  13. Increasing complexity: which drug class to choose for treatment of hypertension in the elderly?

    OpenAIRE

    Kaiser EA; Lotze U; Schäfer HH

    2014-01-01

    Edelgard Anna Kaiser,1 Ulrich Lotze,2 Hans Hendrik Schäfer1,31Roche Diagnostics International AG, Rotkreuz, Switzerland; 2Department of Internal Medicine, DRK-Manniske-Krankenhaus Bad Frankenhausen, Bad Frankenhausen, Germany; 3Institute of Anatomy II, University Hospital Jena, Friedrich-Schiller University, Jena, GermanyAbstract: Treatment of hypertension in the elderly is expected to become more complex in the coming decades. Based on the current landscape of clinical trials, guide...

  14. Introductory note on Emergent Unconscious Knowledge Networks (Asygnodic Networks

    Directory of Open Access Journals (Sweden)

    Henry Bakis

    2016-05-01

    Full Text Available This note introduces the following paper on the concept of Emergent Unconscious Knowledge Networks (Asygnodic Networks created by E. Roche and M. Blaine. The concept of asyngnosis explains a large number of diverse phenomena involving organizations, groups and decision making. It will present the genesis and definition of Asygnodic Networks and will focus on how they raise challenges to traditional theories of decision making and emerging social networks.

  15. Binary evolution using the theory of osculating orbits: conservative Algol evolution

    OpenAIRE

    Davis, P J; Siess, L.; Deschamps, R.

    2014-01-01

    Our aim is to calculate the evolution of Algol binaries within the framework of the osculating orbital theory, which considers the perturbing forces acting on the orbit of each star arising from mass exchange via Roche lobe overflow (RLOF). The scheme is compared to results calculated from a `classical' prescription. Using our stellar binary evolution code BINSTAR, we calculate the orbital evolution of Algol binaries undergoing case A and case B mass transfer, by applying the osculating schem...

  16. Erziehungstheorie, Literatur und Geschlecht Educational Theory, Literature, and Gender

    OpenAIRE

    Barbara Gribnitz

    2007-01-01

    Die Verfasserin stellt sich die Aufgabe, das „erkenntnistheoretische Potential des literarischen Textes als Quelle historisch-pädagogischer Forschung“ (S. 12) am Beispiel des Genre Bildungsroman herauszuarbeiten. Anhand zweier Romane, die weibliche Lebensläufe beschreiben (Sophie von La Roche: Die Geschichte des Fräuleins von Sternheim; Friederike Helene Unger: Julchen Grünthal), erweitert sie die Bildungsromandebatte in der Erziehungs- und Literaturwissenschaft um die Kategorie Geschlecht un...

  17. Marketingová komunikace divize aktivní kosmetiky společnosti L'Oréal

    OpenAIRE

    Butakova, Anastasia

    2014-01-01

    Bachelor's thesis deals with analysis of marketing communications active cosmetics division L'Oréal group at the example of Vichy and La Roche-Posay brands. The goal is to evaluate communication activities of these brands and its effects. In the theoretical section are defined such terms as communication mix, brand, consumer purchase decision process; the practical part is focused on the market analysis obtained from the questionnaire survey.

  18. Analýza nákupního chování spotřebitelů na trhu dermokosmetiky

    OpenAIRE

    Kolínská, Aneta

    2013-01-01

    The thesis focuses on the buying behavior of consumers at the dermocosmetics market in the Czech Republic with a main focus on consumers of brands Vichy, Bioderma, La Roche-Posay, Avene and Eucerin. The work is divided into two main parts, theoretical and practical. The first part is devoted to general theoretical knowledge in the field of shopping behavior of consumers, the buying decision process and its various stages. It also builds on the theoretical foundation of marketing research, its...

  19. Holomorphic Morse inequalities and asymptotic cohomology groups: a tribute to Bernhard Riemann

    CERN Document Server

    Demailly, Jean-Pierre

    2010-01-01

    The goal of this note is to present the potential relationships between certain Monge-Amp\\`ere integrals appearing in holomorphic Morse inequalities, and asymptotic cohomology estimates for tensor powers of line bundles, as recently introduced by algebraic geometers. The expected most general statements are still conjectural and owe a debt to Riemann's pioneering work, which led to the concept of Hilbert polynomials and to the Hirzebruch-Riemann-Roch formula during the XX-th century.

  20. Mikroskopische Studien ueber Gesteine aus den Molukken. 1. Gesteine von Ambon und den Uliassern. Mit einem französischen Résumé

    NARCIS (Netherlands)

    Schroeder van der Kolk, J.L.C.

    1888-01-01

    Dans le traité précédent j’ai décrit les types les plus caractéristiques parmi les échantillons de roche recueillis par M. MARTIN pendant ses voyages dans les Moluques. Ainsi il s’agit des Granites à biotite, des Péridotites, des Dacites, des Andésites à pyroxène, des Brèches volcaniques et aussi de

  1. 希尔顿全球与锦江国际理诺士酒店管理学院签订里程碑式协议

    Institute of Scientific and Technical Information of China (English)

    2011-01-01

    11月30日,希尔顿全球(HiltonWorldwide)与锦江国际理诺士酒店管理学院(Les Roches lin Jiang International Hotel Management College,简称LRJJ)签署了一项战略合作协议,旨在为迅速扩张的中国酒店业培养人才。

  2. Detection of Chlamydia trachomatis in urine samples by nucleic acid tests: comparison with culture and enzyme immunoassay of genital swab specimens.

    OpenAIRE

    Schepetiuk, S.; Kok, T.; Martin, L; Waddell, R; Higgins, G

    1997-01-01

    Two commercially available nucleic acid-based tests, ligase chain reaction (LCR; Abbott Laboratories) and PCR (Roche Diagnostics), for the detection of Chlamydia trachomatis in male and female urine samples were compared with culture and enzyme immunoassay (EIA) (Microtrak; Syva) for C. trachomatis detection in genital samples. The samples were collected from 1,005 patients who attended a sexually transmitted disease clinic. In this study population, the prevalence of the infection was 4%. Sp...

  3. Pilot Study of COBAS PCR and Ligase Chain Reaction for Detection of Rectal Infections Due to Chlamydia trachomatis

    OpenAIRE

    Matthew R Golden; Astete, Sabina G.; Galvan, Rosa; Lucchetti, Aldo; Sanchez, Jorge; Celum, Connie L.; Whittington, William L. H.; Stamm, Walter E.; Holmes, King K.; Totten, Patricia A.

    2003-01-01

    We tested rectal specimens from men who have sex with men for Chlamydia trachomatis by using COBAS PCR (Roche Diagnostics) and ligase chain reaction LCR (Abbott laboratories) and compared three PCR specimen-processing procedures. Chlamydiae were detected by one or more procedures in 22 of 186 specimens. All three PCR tests were positive for 17 specimens, all of which also tested positive by LCR.

  4. Evaluation of four gentamicin and tobramycin assay procedures for clinical laboratories.

    OpenAIRE

    Witebsky, F G; Sliva, C A; Selepak, S T; Ruddel, M E; MacLowry, J D; Johnson, E E; Elin, R J

    1983-01-01

    Accuracy, precision, and clinical laboratory utility of the TDX (Abbott Laboratories), Auto-ICS (Beckman Instruments, Inc.), COBAS-Bio (Roche Analytical Instruments, Inc.) with reagent kits (Syva), and EMIT (Syva) for gentamicin and tobramycin serum assay were assessed. TDX, COBAS-Bio, and EMIT analytical systems showed a proportional bias of less than 10% for recovery studies and a coefficient of variation less than 5% for within-run precision. The results of the recovery studies with the Au...

  5. Intestinal Dysbiosis and Depletion of Butyrogenic Bacteria in Clostridium difficile Infection and Nosocomial Diarrhea

    OpenAIRE

    Antharam, Vijay C.; Li, Eric C.; Ishmael, Arif; Sharma, Anuj; Mai, Volker; Rand, Kenneth H.; Wang, Gary P.

    2013-01-01

    Clostridium difficile infection (CDI) causes nearly half a million cases of diarrhea and colitis in the United States each year. Although the importance of the gut microbiota in C. difficile pathogenesis is well recognized, components of the human gut flora critical for colonization resistance are not known. Culture-independent high-density Roche 454 pyrosequencing was used to survey the distal gut microbiota for 39 individuals with CDI, 36 subjects with C. difficile-negative nosocomial diarr...

  6. SWIFT J1749.4-2807: A neutron or quark star?

    Institute of Scientific and Technical Information of China (English)

    Jun-Wei Yu; Ren-Xin Xu

    2010-01-01

    We investigate a unique accreting millisecond pulsar with X-ray eclipses,SWIFT J1749.4-2807(hereafter J1749),and try to set limits on the binary system by various methods including use of the Roche lobe,the mass-radius relations of both main sequence(MS)and white dwarf(WD)companion stars,as well as the measured mass function of the pulsar.The calculations are based on the assumption that the radius of the companion star has reached its Roche radius(or is at 90%),but the pulsar's mass has not been assumed to be a certain value.Our results are as follows.The companion star should be an MS one.For the case that the radius equals its Roche one,we have a companion star with mass M(~-)0.51 M⊙ and radius Rc(~-)0.52 R⊙,and the inclination angle is i(~-)76.5°; for the case that the radius reaches 90% of its Roche one,we have M(~-)0.43 M⊙,Re()0.44 R⊙ and i(~-)75.7°.We also obtain the mass of J1749,Mp(~-)1 M⊙,and conclude that the pulsar could be a quark star if the ratio of the critical frequency of rotation-mode instability to the Keplerian one is higher than~0.3.The relatively low pulsar mass(about~M⊙)may also challenge the conventional recycling scenario for the origin and evolution of millisecond pulsars.The results presented in this paper are expected to be tested by future observations.

  7. Halting Planetary Migration

    OpenAIRE

    Lecar, M.; Sasselov, D. D.

    1999-01-01

    When Jupiter's Roche Lobe radius exceeded the scale height of the protoplanetary disk, Jupiter opened a gap in the disk. When the gap was wide enough, tidal torques from the disk interior and exterior to Jupiter were suppressed and migration continued on the accretion time scale. In the 'minimum solar nebula' about two Jupiter masses of gas remained between Jupiter and Saturn and about five Jupiter masses between Jupiter and Uranus. Unless all but a Jupiter mass of the outer disk was removed,...

  8. Transcriptome Analysis of Sarracenia, an Insectivorous Plant

    OpenAIRE

    Srivastava, Anuj; Rogers, Willie L.; Breton, Catherine M.; Cai, Liming; Malmberg, Russell L.

    2011-01-01

    Sarracenia species (pitcher plants) are carnivorous plants which obtain a portion of their nutrients from insects captured in the pitchers. To investigate these plants, we sequenced the transcriptome of two species, Sarracenia psittacina and Sarracenia purpurea, using Roche 454 pyrosequencing technology. We obtained 46 275 and 36 681 contigs by de novo assembly methods for S. psittacina and S. purpurea, respectively, and further identified 16 163 orthologous contigs between them. Estimation o...

  9. Comparison of Next-Generation Sequencing Systems

    OpenAIRE

    Lin Liu; Yinhu Li; Siliang Li; Ni Hu; Yimin He; Ray Pong; Danni Lin; Lihua Lu; Maggie Law

    2012-01-01

    With fast development and wide applications of next-generation sequencing (NGS) technologies, genomic sequence information is within reach to aid the achievement of goals to decode life mysteries, make better crops, detect pathogens, and improve life qualities. NGS systems are typically represented by SOLiD/Ion Torrent PGM from Life Sciences, Genome Analyzer/HiSeq 2000/MiSeq from Illumina, and GS FLX Titanium/GS Junior from Roche. Beijing Genomics Institute (BGI), which possesses the world’s ...

  10. Massively Parallel Sequencing Approaches for Characterization of Structural Variation

    OpenAIRE

    Koboldt, Daniel C.; Larson, David E.; Chen, Ken; Ding, Li; Wilson, Richard K.

    2012-01-01

    The emergence of next-generation sequencing (NGS) technologies offers an incredible opportunity to comprehensively study DNA sequence variation in human genomes. Commercially available platforms from Roche (454), Illumina (Genome Analyzer and Hiseq 2000), and Applied Biosystems (SOLiD) have the capability to completely sequence individual genomes to high levels of coverage. NGS data is particularly advantageous for the study of structural variation (SV) because it offers the sensitivity to de...

  11. Integrated Core Facility Support and Optimization of Next Generation Sequencing Technologies

    OpenAIRE

    Schweitzer, P.; Sun, Q; Pillardy, J.; Wang, W.; Stelick, T.; Bukowski, R; Ponnala, L.; VanEe, J.; Grills, G.

    2011-01-01

    New DNA sequencing technologies present an exceptional opportunity for novel and creative applications with the potential for breakthrough discoveries. To support such research efforts, the Cornell University Life Sciences Core Laboratories Center has implemented the Illumina HiSeq 2000 and the Roche 454 GS FLX platforms as academic core facility shared research resources. We have established sample handling methods, LIMS tools and BioHPC informatics analysis pipelines in support of these new...

  12. A new Vitreoscilla filiformis extract grown on spa water-enriched medium activates endogenous cutaneous antioxidant and antimicrobial defenses through a potential Toll-like receptor 2/protein kinase C, zeta transduction pathway

    OpenAIRE

    Mahe YF; Perez MJ; Tacheau C; Fanchon C; Martin R.; Rousset F.; Seite S

    2013-01-01

    Yann F Mahe,1 Marie-Jesus Perez,1 Charlotte Tacheau,1 Chantal Fanchon,2 Richard Martin,3 Françoise Rousset,1 Sophie Seite4 1L’Oreal Research and Innovation, Clichy, 2L’Oréal Research and Innovation, Chevilly Larue, 3L’Oréal Research and Innovation Tours, 4La Roche-Posay Pharmaceutical Laboratories, Asnières, France Abstract: Vitreoscilla filiformis (VF) biomass (VFB) has been widely used in cosmetic preparations and sh...

  13. Draft genome of the globally widespread and invasive Argentine ant (Linepithema humile)

    OpenAIRE

    Smith, Christopher D.; Zimin, Aleksey; Holt, Carson; Abouheif, Ehab; Benton, Richard; Cash, Elizabeth; Croset, Vincent; Currie, Cameron R; Elhaik, Eran; Elsik, Christine G.; Fave, Marie-Julie; Fernandes, Vilaiwan; Gadau, Jürgen; Gibson, Joshua D.; Graur, Dan

    2011-01-01

    Ants are some of the most abundant and familiar animals on Earth, and they play vital roles in most terrestrial ecosystems. Although all ants are eusocial, and display a variety of complex and fascinating behaviors, few genomic resources exist for them. Here, we report the draft genome sequence of a particularly widespread and well-studied species, the invasive Argentine ant (Linepithema humile), which was accomplished using a combination of 454 (Roche) and Illumina sequencing and community-b...

  14. Thermonuclear Burning on the Accreting X-Ray Pulsar GRO J1744-28

    CERN Document Server

    Bildsten, L; Bildsten, Lars; Brown, Edward F.

    1996-01-01

    We investigate the thermal stability of nuclear burning on the accreting X-ray pulsar GRO J1744-28. The neutron star's dipolar magnetic field is 50 years. We also discuss the nature of the binary and point out that a velocity measurement of the stellar companion (most likely a Roche-lobe filling giant with m_K>17) will constrain the neutron star mass.

  15. Recommended Nordic paediatric reference intervals for 21 common biochemical properties

    DEFF Research Database (Denmark)

    Hilsted, Linda; Rustad, Pål; Aksglæde, Lise;

    2013-01-01

    healthy Danish children were collected for establishing reference intervals for 21 common biochemical properties (Alanine transaminase, Albumin, Alkaline phosphatase, Aspartate transaminase, Bilirubin, Calcium, Cholesterol, Creatinine, Creatine kinase, HDL-Cholesterol, Iron, Lactate dehydrogenase, LDL......- Cholesterol, Magnesium, Phosphate, Potassium, Protein, Sodium, Transferrin, Triglycerides and Urate). Samples were analyzed on a Roche-Modular-P/ISE-system. The NORIP reference material (NFKK Reference Serum X) was included in all the analytical runs. Reference values were recalculated according to the target...

  16. Feasibility Analysis For Heating Tribal Buildings with Biomass

    Energy Technology Data Exchange (ETDEWEB)

    Steve Clairmont; Micky Bourdon; Tom Roche; Colene Frye

    2009-03-03

    This report provides a feasibility study for the heating of Tribal buildings using woody biomass. The study was conducted for the Confederated Salish and Kootenai Tribes of the Flathead Reservation in western Montana. S&K Holding Company and TP Roche Company completed the study and worked together to provide the final report. This project was funded by the DOE's Tribal Energy Program.

  17. Second class citizens...second class women: The impact of gender inequality and gender based violence on transsexual women

    OpenAIRE

    Roch, Amy

    2012-01-01

    Background: In 2010 the LGBT Domestic Abuse Project and the Scottish Transgender Alliance published research looking at the transgender people's experience of domestic abuse (Roch et al, 2010). The research found extremely high levels of domestic abuse and lifted the lid on an area of gender based violence that we knew little about in Scotland. One of the most worrying findings from the research, however, related not to domestic abuse, but to another form of gender based violence. When resp...

  18. Low Diversity in the Mitogenome of Sperm Whales Revealed by Next-Generation Sequencing

    OpenAIRE

    Alexander, Alana; Steel, Debbie; Slikas, Beth; Hoekzema, Kendra; Carraher, Colm; Parks, Matthew; Cronn, Richard; Baker, C. Scott

    2012-01-01

    Large population sizes and global distributions generally associate with high mitochondrial DNA control region (CR) diversity. The sperm whale (Physeter macrocephalus) is an exception, showing low CR diversity relative to other cetaceans; however, diversity levels throughout the remainder of the sperm whale mitogenome are unknown. We sequenced 20 mitogenomes from 17 sperm whales representative of worldwide diversity using Next Generation Sequencing (NGS) technologies (Illumina GAIIx, Roche 45...

  19. Ruwenzori, Montagnes de la Lune

    OpenAIRE

    Sesiano, Jean

    1990-01-01

    Une expédition au Ruwenzori, point culminant des Montagnes de la Lune, par le Zaïre, a permis quelques observations de géomorphologie glaciaire, dans ce massif de roches métamorphiques culminant à plus de 5000 m. Localisé sur l'équateur, il reçoit d'abondantes précipitations.

  20. Avaliação da infestação e diversidade de Teredinidae (Mollusca - Bivalvia) ao longo da costa do estado do Rio de Janeiro, Brasil Evaluation of the infestation and diversity of Teredinidae (Mollusca - Bivalvia) along the coast of Rio de Janeiro State, Brazil

    OpenAIRE

    Junqueira, Andrea O. R.; Sergio Henrique G. da Silva; Maria Júlia M. Silva

    1989-01-01

    In order to evaluate the diversity and infestation level of Teredinidae wood-boring molluscs, ten satations were chosen along the coast of Rio de Janeiro State. Using five pine-wood panels in each station, it was possible to distinguish five areas according to species dominance. Ilha Grande Bay is characterized by the dominance of Lyrodus floridanus (Bartsch, 1922); Sepetiba Bay, by Bankia fimbriatula Moll & Roch, 1931, Tijuca Lagoon by Teredo navalis Linnaeus, 1758; Guanabara Bay by Notoredo...

  1. Macroprolactin as a Cause of Hyperprolactinemia: Clinical and Radiological Features

    OpenAIRE

    Assim Alfadda

    2008-01-01

    Objective: The aim of this study was to determine the prevalence of macroprolactin in patients with hyperprolactinemia in our region, and to determine the clinical and neuroradiological features of the affected individuals. Materials and Methods: We used the Roche Elecsys Prolactin assay (Prolactin II) with polyethylene glycol precipitation to identify macroprolactin; recovery of ≤40% was considered to represent significant macroprolactinemia. Of 156 consecutive patients with hyperprolactinem...

  2. Orbital Evolution and Migration of Giant Planets: Modeling Extrasolar Planets

    OpenAIRE

    Trilling, D. E.; Benz, W.; Guillot, T.; Lunine, J. I.; Hubbard, W. B.; Burrows, A.

    1998-01-01

    Giant planets in circumstellar disks can migrate inward from their initial (formation) positions. Radial migration is caused by inward torques between the planet and the disk; by outward torques between the planet and the spinning star; and by outward torques due to Roche lobe overflow and consequent mass loss from the planet. We present self-consistent numerical considerations of the problem of migrating giant planets. Summing torques on planets for various physical parameters, we find that ...

  3. Administrative risk quantification of subcutaneous and intravenous therapies in Italian centers utilizing the Failure Mode and Effects Analysis approach

    OpenAIRE

    Walzer, Stefan; Ponzetti,Clemente; Canciani,Monica; Farina,Massimo; Era, Sara

    2016-01-01

    Clemente Ponzetti,1 Monica Canciani,2 Massimo Farina,2 Sara Era,3 Stefan Walzer4,5 1Gruppo Policlinico di Monza, Alessandria, ANMDO National Association of Hospital Physicians, Bologna, 2Studio EmmEffe Srl, Milan, 3Roche Spa, Monza, Italy; 4MArS Market Access & Pricing Strategy GmbH, Weil am Rhein, 5State University Baden-Wuerttemberg, Health Care Management, Loerrach, Germany Background: In oncology, an important parameter of safety is the potential treatment error in hospitals. The ...

  4. Describing and Transcribing the Phonologies of the Amdo Sprachbund

    OpenAIRE

    Juha Janhunen (translator)

    2015-01-01

    Juha Janhunen. 2015. Describing and Transcribing the Phonologies of the Amdo Sprachbund in Gerald Roche, Keith Dede, Fernanda Pirie, and Benedict Copps (eds) Asian Highlands Perspectives 37 Centering the Local, A Festschrift for Dr. Charles Kevin Stuart on the Occasion of his Sixtieth Birthday, 122-137. The Amdo Qinghai region, at the frontier of Tibet and China, comprises languages belonging to three genetic language families (Sino-Tibetan, Turkic, and Mongolic), three typologi...

  5. ETA INVARIANTS, DIFFERENTIAL CHARACTERS AND FLAT VECTOR BUNDLES

    Institute of Scientific and Technical Information of China (English)

    J.M.BISMUT

    2005-01-01

    The purpose of this paper is to give a refinement of the Atiyah-Singer families index theorem at the level of differential characters. Also a Riemann-Roch-Grothendieck theorem for the direct image of flat vector bundles by proper submersions is proved,with Chern classes with coefficients in C/Q. These results are much related to prior work of Gillet-Soule, Bismut-Lott and Lott.

  6. LUT observations of the mass-transferring binary AI Dra

    Science.gov (United States)

    Liao, Wenping; Qian, Shengbang; Li, Linjia; Zhou, Xiao; Zhao, Ergang; Liu, Nianping

    2016-06-01

    Complete UV band light curve of the eclipsing binary AI Dra was observed with the Lunar-based Ultraviolet Telescope (LUT) in October 2014. It is very useful to adopt this continuous and uninterrupted light curve to determine physical and orbital parameters of the binary system. Photometric solutions of the spot model are obtained by using the W-D (Wilson and Devinney) method. It is confirmed that AI Dra is a semi-detached binary with secondary component filling its critical Roche lobe, which indicates that a mass transfer from the secondary component to the primary one should happen. Orbital period analysis based on all available eclipse times suggests a secular period increase and two cyclic variations. The secular period increase was interpreted by mass transfer from the secondary component to the primary one at a rate of 4.12 ×10^{-8}M_{⊙}/yr, which is in agreement with the photometric solutions. Two cyclic oscillations were due to light travel-time effect (LTTE) via the presence of two cool stellar companions in a near 2:1 mean-motion resonance. Both photometric solutions and orbital period analysis confirm that AI Dra is a mass-transferring binary, the massive primary is filling 69 % of its critical Roche lobe. After the primary evolves to fill the critical Roche lobe, the mass transfer will be reversed and the binary will evolve into a contact configuration.

  7. Nature of the Extreme Ultraluminous X-ray Sources

    CERN Document Server

    Wiktorowicz, Grzegorz; Sadowski, Aleksander; Belczynski, Krzysztof

    2015-01-01

    In this proof-of-concept study we demonstrate how a binary system can easily form an extreme ULX source with the X-ray luminosity of L_x > 10^42 erg/s. Formation efficiencies and lifetimes of such objects are high enough to potentially explain all observed extreme ULXs. These systems are not only limited to binaries with stellar-origin black hole accretors. Noteworthy, we have also identified such objects with neutron stars. Typically, a 10 Msun black hole is fed by a massive (~10 Msun) Hertzsprung gap donor with Roche lobe overflow rate of ~10^-3 Msun/yr (~2600 Mdot_Edd). For neutron star systems the typical donors are evolved low-mass (~2 Msun) helium stars with Roche lobe overflow rate of ~10^-2 Msun/yr. We base our study purely on the available Roche lobe overflow rate in a binary system and show that if only even a small fraction (>10^-3) of the overflow reaches the BH, the source will be super-Eddington. Our study does not prove that any particular extreme ULX (e.g., HLX-1) is a regular binary system wi...

  8. The narrow rings of Jupiter, Saturn and Uranus

    Science.gov (United States)

    Dermott, S. F.; Murray, C. D.; Sinclair, A. T.

    1980-01-01

    The origin of the newly discovered narrow ring systems around Jupiter, Saturn and Uranus is considered. It is pointed out that both the Uranian and Jovian ring systems have mean orbital radii of 1.8 planetary radii and lie within the Roche zones of their respective planets, and it is suggested that the Jovian ring is the product of the disintegration of a satellite that entered the Roche zone, and that large numbers of small particles are now in horseshoe orbits about the Lagrangian equilibrium points of the remnant chunks. Analysis of the path of a ring particle in a horseshoe orbit is shown to result in ring structures in agreement with those observed for the circular rings of Jupiter and the highly eccentric ring of Uranus. The stability of these ring systems is then considered, and it is suggested that the F ring of Saturn, which lies outside the Roche zone, represents primordial matter not yet accreted by small satellites just inside the Mimas first-order resonances.

  9. Instability of mass transfer in a planet-star system

    CERN Document Server

    Jia, Shi

    2016-01-01

    We show that the angular momentum exchange mechanism governing the evolution of mass transferring binary stars does not apply to Roche-lobe filling planets, because most of the angular momentum of the mass transferring stream is absorbed by the host star. Apart from a correction for the difference in specific angular momentum of the stream and the centre of mass of the planet, the orbit does not expand much on Roche-lobe overflow. We explore the conditions for dynamically unstable Roche-lobe overflow as a function of planet mass and mass and radius (age) of host star and equation of state of planet. For a Sun-like host, gas giant planets in a range of mass and entropy can undergo dynamical mass transfer. Examples of the evolution of the mass transfer process are given. Dynamic mass transfer of rocky planets depend somewhat sensitively on equation of state used. Silicate planets in the range $1 M_{\\oplus}

  10. Mass-Loss Rates of "Hot-Jupiter" Exoplanets with Various Types of Gaseous Envelopes

    CERN Document Server

    Cherenkov, A A; Kaigorodov, P V

    2016-01-01

    According to the computations results obtained by Bisikalo et al. (2013b) for the gas-dynamical effect of stellar winds on exoplanet atmospheres, three types of gaseous envelopes can form around hot Jupiters: closed, quasi-closed, and open. The type of envelope that forms depends on the position of the frontal collision point (where the dynamical pressure of the wind is equal to the pressure of the surrounding atmosphere) relative to the Roche-lobe boundaries. Closed envelopes are formed around planets whose atmospheres lie completely within their Roche lobes. If the frontal collision point is located outside the Roche lobe, the atmospheric material begins to flow out through the Lagrangian points $\\mathrm{L_1}$ and $\\mathrm{L_2}$, which can result in the formation of quasi-closed (if the dynamical pressure of the stellar wind stops the outflow through $\\mathrm{L_1}$) or open gaseous envelopes. The example of the typical hot Jupiter HD 209458 b is considered for four sets of atmospheric parameters, to determi...

  11. A new Vitreoscilla filiformis extract grown on spa water-enriched medium activates endogenous cutaneous antioxidant and antimicrobial defenses through a potential Toll-like receptor 2/protein kinase C, zeta transduction pathway

    Directory of Open Access Journals (Sweden)

    Mahe YF

    2013-08-01

    Full Text Available Yann F Mahe,1 Marie-Jesus Perez,1 Charlotte Tacheau,1 Chantal Fanchon,2 Richard Martin,3 Françoise Rousset,1 Sophie Seite4 1L’Oreal Research and Innovation, Clichy, 2L’Oréal Research and Innovation, Chevilly Larue, 3L’Oréal Research and Innovation Tours, 4La Roche-Posay Pharmaceutical Laboratories, Asnières, France Abstract: Vitreoscilla filiformis (VF biomass (VFB has been widely used in cosmetic preparations and shown to modulate the major inducible free-radical scavenger mitochondrial superoxide dismutase in skin cells. By adding La Roche-Posay (LRP thermal spring water to the VF culture medium, we obtained a biomass (LRP-VFB with a similar mitochondrial superoxide dismutase activation capacity to VF. Also, the new biomass more powerfully stimulated mRNA expression and antimicrobial peptides in reconstructed epidermis. Interestingly, a predictive computer model that analyzed transducing events within skin epidermal cells suggested that this protective activity may involve the Toll-like receptor 2/protein kinase C, zeta transduction pathway. Protein kinase C, zeta inhibition was effectively shown to abolish VFB-induced gene stimulation and confirmed this hypothesis. This thus opens new avenues for investigation into the improvement of skin homeostatic defense in relation to the control of its physiological microbiota and innate immunity. Keywords: innate skin defenses, TLR2, PKCz, La Roche-Posay, mitochondrial superoxide dismutase, SOD2

  12. In situ cosmogenic 10Be dating of the Quaternary glaciations in the southern Shaluli Mountain on the Southeastern Tibetan Plateau

    Institute of Scientific and Technical Information of China (English)

    WANG; Jian; Raisbeck; Grand; XU; Xiaobin; Yiou; Francios; BAI; Shibiao

    2006-01-01

    It is generally considered that four-times ice age happened during the Quaternary epoch on the Tibetan Plateau.However,the research on the chronology of the four-times ice age is far from enough.The Shaluli Mountain on the Southeastern Tibetan Plateau is an ideal place for plaeo-glacier study,because there are abundant Quaternary glacial remains there.This paper discusses the ages of the Quaternary glaciations,based on the exposure dating of roche moutonnée,moraines and glacial erosion surfaces using in situ cosmogenic isotopes 10Be.It is found that the exposure age of the roche moutonnée at Tuershan is 15 ka,corresponding to Stage 2 of the deep-sea oxygen isotope,suggesting that the roche moutonnée at Tuershan is formed in the last glacial maximum.The exposure age of glacial erosion surface at Laolinkou is 130-160 ka,corresponding to Stage 6 of the deep-sea oxygen isotope.The oldest end moraine at Kuzhaori may form at 421-766 kaBP,corresponding to Stages 12-18 of the deep-sea oxygen isotope.In accordance with the climate characteristic of stages 12,14,16 and 18 reflected by the deep-sea oxygen isotope,polar ice cores and loess sequence,the oldest end moraine at Kuzhaori may form at stage 12 or stage 16,the latter is more possible.

  13. 三种不同方法测定糖化血红蛋白的结果比对和偏移评估分析%Comparison and bias estimation of three methods in determination of glycated hemoglobin A1c

    Institute of Scientific and Technical Information of China (English)

    林莉; 戴永辉; 刘冬冬; 黄景春; 石文; 尚陈宇; 潘婉仪; 邱峰; 徐建华

    2016-01-01

    Objective To evaluate the bias of three different methods in determination of glycated hemoglobin A1 c (HbA1 c).Methods According to Clinical and Laboratory Standards Institute (CLSI) EP9-A3 document,40 serum specimens among linear range were tested by immunoturbidimetry assay (Roche Tina-quant),capillary electrophoresis (Sebia Minicap FP),high performance liquid chromatography system (HPLC,Trinity Biotech Premier Hb 9210TM).Results were analyzed by pairwise comparision.The difference plots and scatter plots were analyzed.The results of outliers were examined by extreme studentized deviate (ESD) method.The best regression model was chosen to fit the regression equation and the biases were calculated at the level of medical decision and judged the comparability according to the half of TEa acceptance standard.Results Scatter plots and ESD results showed good correlation among test results of 40 samples,no abnormal values were found.Based on proportional difference deviation plots,proportional difference rank order deviation plots,numerical deviation plots and ranked order difference plots,weighted least squares (WLS) (Roche (X) vs sebia (Y1)),ordinary linear regression (OLR) (Roche(X)vs Trinity (Y2)) and Deming (Sebia(Y1)vs Trinity(Y2))were chosen to fit regression analysis model.The regression equations were Y1 =-0.420 3 + 1.052 7X,Y2 =-0.174 0 + 1.027 0X,Y2 =0.423 1 + 0.959 0Y1,respectively.Assuming the HbA1c medical decision points of 10%,16% to regression equation,the proportional biases were 1.06% and 2.61% at Roche (X)and sebia(Y1) system,0.96% and 1.60% at Roche(X) and Trinity (Y2) system,0.13% and-1.47% at Sebia (Y1)and Trinity (Y2)system respectively,which were all lower than the acceptable values.Conclusion The measurement values of HbA1c by capillary electrophoresis (Sebia Minieap FP),HPLC (Trinity Biotech Premier Hb 9210TM) and immunoturbidimetry assay (Roche Tina-quant),are comparable.%目的 对3种不同原理

  14. Reference intervals for common thyroid function tests, during different stages of pregnancy in Chinese women

    Institute of Scientific and Technical Information of China (English)

    FAN Jian-xia; HAN Mi; TAO Jun; LUO Jun; SONG Meng-fan; YANG Shuai; KHOR Shu-zin

    2013-01-01

    Background The importance of diagnosis of thyroid dysfunction during pregnancy has been widely recognized.Our study was designed to compare two different detection reagents between Abbott and Roche and to establish the gestational-related reference intervals for thyroid function tests (TFT) in Chinese women and to assay the reference ranges with the American Thyroid Association recommended standard.Methods Serum samples were collected from 693 normal pregnant Chinese women and divided into five groups according to their gestational age:9-13,16-20,24-28,32-34 and 37-40 weeks.Thyroid stimulating hormone (TSH) and free thyroxine (FT4) levels were determined by two different detection reagents:Abbott Architect l 2000 and Roche Cobas Elecsys 600.The reference ranges of the TFT indexes were calculated according to the National Academy of Clinical Biochemistry (NACB).The 2.5th and 97.5th percentiles of each stage were calculated,and the results were analyzed by one-way analysis of variances,t-test,and Spearman correlation analysis.Results Thyroid hormone levels varied greatly among different gestational stages.TSH levels,as assessed via two different TSH ELISA kits showed consistent changing pattern during pregnancy and displayed linear correlation (P <0.001).In 9-13 gestational weeks,TSH levels were significantly lower than that of other groups; and in 37-40 gestational weeks,it was higher than that of other groups (all P <0.001).TSH reference ranges determined by Roche detection reagent in each group were higher than those by Abbott detection reagent (P <0.01 respectively).FT4 levels were higher in 9-13 gestational weeks than that of other groups (P <0.001).FT4 levels determined by Roche reagent were higher than Abbott reagent in 9-13 weeks,(P <0.001),and lower in 24-28 and 37-40 weeks (P <0.001 and P=0.016,respectively).The TSH level was correlated with FT4 levels in 9-13 gestational weeks by detection reagents (for Abbott reagent,r=-0.319 for FT4 P <0

  15. Síndrome da meningite asséptica por enterovírus e Leptospira sp em crianças de Salvador, Bahia

    Directory of Open Access Journals (Sweden)

    Silva Hagamenon R.

    2002-01-01

    Full Text Available Objetivando verificar a freqüência de enterovírus (EV, leptospiras e arbovírus como agentes causais da síndrome da meningite asséptica (SMA, em períodos não-epidêmicos, e comparar os pacientes com e sem diagnóstico etiológico determinado, foram selecionados 112 pacientes de idade entre 3 meses e 15 anos, com suspeita clínica de SMA, referenciados para Hospital Couto Maia, especializado em Doenças Infecciosas e Parasitárias (Salvador, Bahia, Em 44,6% (n=50 a etiologia foi determinada: enterovírus em 37,7% (n=42 dos casos, pelo teste de PCR Amplicor, por cultura do líquor e/ou de fezes; a Leptospira sp. em 7,1% (n=8, pelo método da micro-aglutinação, e nenhum caso de arbovírus foi detectado (inibição da hemaglutinação passiva. Entre os 14 enterovírus dos 22 isolados, foram identificados seis diferentes sorotipos, sendo o Echovirus-4 predominante (27,2%; 6/22 entre outros (Coxsackie B2, B3, B6 e B9; EV 71. Conclui-se que, os enterovírus foram os agentes mais freqüentes, e que a leptospirose deve ser lembrada no diagnóstico diferencial da SMA. Uma vez que as características clínicas e liquóricas dos pacientes dos grupos com e sem determinação do agente etiológico foram semelhantes, pode-se supor que o diagnóstico presuntivo de SMA é de provável etiologia viral ou pela leptospira.

  16. Therapy of chronic hepatitis C: Virologic response monitoring

    Directory of Open Access Journals (Sweden)

    Kuljić-Kapulica Nada

    2010-01-01

    Full Text Available Background/Aim. Virological testing is considered to be essential in the management of hepatitis C virus (HCV infection in order to diagnose infection, and, most importantly, as a quide for treatment decisions and assess the virological response to antiviral therapy. The aim of this study was to determine the rate of a sustained virological response (SVR and various factors associated with response rates in chronic hepatitis C infected patients treated with pegiinterferon alpha (PEGINF and ribavirin (RBV combination therapy. Methods. A total of 34 patients, treated with PEG-IFN and RBV were studied. Serum HCV-RNA was measured before the treatment, 12 weeks following the start of the therapy and 6 weeks after the treatment cessation. SVR was defined as undetectable serum HCV-RNA 6 months of post-treatment follow-up, virologic relapse (VR as relapse of HCV-RNA during the post-treatment follow-up. Serum HCV-RNA was measured with the Cobas Amplicor test. Results. At the end of post-treatment follow-up 19 (55.8% patients demonstrated a SVR. The majority of the patients were genotype 1 (27, and the other were genotype 3 (5 patients and genotype 4 (2 patients. There was VR in 6 patients 6 months after the therapy. In 9 patients HCV-RNA was positive after 12 weeks. Conclusion. We demonstrated that patients with chronic HCV infection can be successfully treated with combination of PEG-INF and RBV. This result emphasizes also that post-treatment follow-up to identify patients with SVR or VR could be important.

  17. Human papillomavirus prevalence among indigenous and non-indigenous Australian women prior to a national HPV vaccination program

    Directory of Open Access Journals (Sweden)

    Condon John R

    2011-09-01

    Full Text Available Abstract Background Indigenous women in Australia have a disproportionate burden of cervical cancer despite a national cervical screening program. Prior to introduction of a national human papilloma virus (HPV vaccination program, we determined HPV genotype prevalence by Indigenous status and residence in remote areas. Methods We recruited women aged 17 to 40 years presenting to community-based primary health services for routine Pap screening across Australia. A liquid-based cytology (LBC cervical specimen was tested for HPV DNA using the AMPLICOR HPV-DNA test and a PGMY09/11-based HPV consensus PCR; positive specimens were typed by reverse hybridization. We calculated age-adjusted prevalence by weighting to relevant population data, and determined predictors of HPV-DNA positivity by age, Indigenous status and area of residence using logistic regression. Results Of 2152 women (655 Indigenous, prevalence of the high-risk HPV genotypes was similar for Indigenous and non-Indigenous women (HPV 16 was 9.4% and 10.5%, respectively; HPV 18 was 4.1% and 3.8%, respectively, and did not differ by age group. In younger age groups, the prevalence of other genotypes also did not differ, but in those aged 31 to 40 years, HPV prevalence was higher for Indigenous women (35% versus 22.5%; P Conclusion Although we found no difference in the prevalence of HPV16/18 among Australian women by Indigenous status or, for Indigenous women, residence in remote regions, differences were found in the prevalence of risk factors and some other HPV genotypes. This reinforces the importance of cervical screening as a complement to vaccination for all women, and the value of baseline data on HPV genotype prevalence by Indigenous status and residence for the monitoring of vaccine impact.

  18. Performance Characteristics of the TRUGENE HIV-1 Genotyping Kit and the Opengene DNA Sequencing System

    Science.gov (United States)

    Kuritzkes, Daniel R.; Grant, Robert M.; Feorino, Paul; Griswold, Marshal; Hoover, Marie; Young, Russell; Day, Stephen; Lloyd, Jr., Robert M.; Reid, Caroline; Morgan, Gillian F.; Winslow, Dean L.

    2003-01-01

    The TRUGENE HIV-1 Genotyping Kit and OpenGene DNA Sequencing System are designed to sequence the protease (PR)- and reverse transcriptase (RT)-coding regions of human immunodeficiency virus type 1 (HIV-1) pol. Studies were undertaken to determine the accuracy of this assay system in detecting resistance-associated mutations and to determine the effects of RNA extraction methods, anticoagulants, specimen handling, and potentially interfering substances. Samples were plasma obtained from HIV-infected subjects or seronegative plasma to which viruses derived from wild-type and mutant infectious molecular clones (IMC) of HIV-1 were added. Extraction methods tested included standard and UltraSensitive AMPLICOR HIV-1 MONITOR, QIAGEN viral RNA extraction mini kit, and QIAGEN Ultra HIV extraction kit, and NASBA manual HIV-1 quantitative NucliSens. Sequence data from test sites were compared to a “gold standard” reference sequence to determine the percent agreement. Comparisons between test and reference sequences at the nucleotide level showed 97.5 to 100% agreement. Similar results were obtained regardless of extraction method, regardless of use of EDTA or acid citrate dextrose as anticoagulant, and despite the presence of triglycerides, bilirubin, hemoglobin, antiretroviral drugs, HIV-2, hepatitis C virus (HCV), HBV, cytomegalovirus, human T-cell leukemia virus type 1 (HTLV-1), or HTLV-2. Samples with HIV-1 RNA titers of ≥1,000 copies/ml gave consistent results. The TRUGENE HIV-1 Genotyping Kit and OpenGene DNA Sequencing System consistently generate highly accurate sequence data when tested with IMC-derived HIV and patient samples. PMID:12682150

  19. New Real-Time Reverse Transcriptase-Initiated PCR Assay with Single-Copy Sensitivity for Human Immunodeficiency Virus Type 1 RNA in Plasma

    Science.gov (United States)

    Palmer, Sarah; Wiegand, Ann P.; Maldarelli, Frank; Bazmi, Holly; Mican, JoAnn M.; Polis, Michael; Dewar, Robin L.; Planta, Angeline; Liu, Shuying; Metcalf, Julia A.; Mellors, John W.; Coffin, John M.

    2003-01-01

    More sensitive assays for human immunodeficiency virus type 1 (HIV-1) RNA are needed to detect, quantify, and characterize persistent viremia in patients who are receiving antiretroviral therapy and whose plasma HIV-1 RNA levels are suppressed to less than 50 to 75 copies/ml. We therefore developed an internally controlled real-time reverse transcriptase-initiated PCR assay that quantifies HIV-1 RNA concentrations down to 1 copy per ml of plasma. This assay with single-copy sensitivity (the single-copy assay) generates a reproducible linear regression plot of input copy number versus threshold cycle by using HIV-1 RNA transcripts at copy numbers ranging from 1 to 106 per reaction mixture. The single-copy assay was compared to the ultrasensitive AMPLICOR HIV-1 MONITOR assay and a more sensitive modification of the ultrasensitive assay by repeatedly testing a low-copy-number panel containing 200 to 0.781 copies of HIV-1 RNA per ml of plasma. This comparison showed that the single-copy assay had a greater sensitivity than the other assays and was the only assay that detected HIV-1 RNA at levels as low as 0.781 copies/ml. Testing of plasma samples from 15 patients who were receiving antiretroviral therapy and who had <75 HIV-1 RNA copies/ml revealed persistent viremia in all 15 patients, with HIV-1 RNA levels ranging from 1 to 32 copies/ml (median, 13 copies/ml). The greater sensitivity of the single-copy assay should allow better characterization of persistent viremia in patients who are receiving antiretroviral therapy and whose HIV-1 RNA levels are suppressed to below the detection limits of present assays. PMID:14532178

  20. Association between ocular bacterial carriage and follicular trachoma following mass azithromycin distribution in The Gambia.

    Directory of Open Access Journals (Sweden)

    Sarah E Burr

    Full Text Available BACKGROUND: Trachoma, caused by ocular Chlamydia trachomatis infection, is the leading infectious cause of blindness, but its prevalence is now falling in many countries. As the prevalence falls, an increasing proportion of individuals with clinical signs of follicular trachoma (TF is not infected with C. trachomatis. A recent study in Tanzania suggested that other bacteria may play a role in the persistence of these clinical signs. METHODOLOGY/PRINCIPAL FINDINGS: We examined associations between clinical signs of TF and ocular colonization with four pathogens commonly found in the nasopharnyx, three years after the initiation of mass azithromycin distribution. Children aged 0 to 5 years were randomly selected from 16 Gambian communities. Both eyes of each child were examined and graded for trachoma according to the World Health Organization (WHO simplified system. Two swabs were taken from the right eye: one swab was processed for polymerase chain reaction (PCR using the Amplicor test for detection of C. trachomatis DNA and the second swab was processed by routine bacteriology to assay for the presence of viable Streptococcus pneumoniae, Haemophilus influenzae, Staphylococcus aureus and Moraxella catarrhalis. Prevalence of TF was 6.2% (96/1538 while prevalence of ocular C. trachomatis infection was 1.0% (16/1538. After adjustment, increased odds of TF were observed in the presence of C. trachomatis (OR = 10.4, 95%CI 1.32-81.2, p = 0.03, S. pneumoniae (OR = 2.14, 95%CI 1.03-4.44, p = 0.04 and H. influenzae (OR = 4.72, 95% CI 1.53-14.5, p = 0.01. CONCLUSIONS/SIGNIFICANCE: Clinical signs of TF can persist in communities even when ocular C. trachomatis infection has been controlled through mass azithromycin distribution. In these settings, TF may be associated with ocular colonization with bacteria commonly carried in the nasopharnyx. This may affect the interpretation of impact surveys and the determinations of thresholds for discontinuing mass

  1. Performance of an Early Infant Diagnostic Test, AmpliSens DNA-HIV-FRT, Using Dried Blood Spots Collected from Children Born to Human Immunodeficiency Virus-Infected Mothers in Ukraine

    Science.gov (United States)

    Shanmugam, Vedapuri; Azarskova, Marianna; Nguyen, Shon; Hurlston, Mackenzie; Sabatier, Jennifer; Zhang, Guoqing; Osmanov, Saladin; Ellenberger, Dennis; Yang, Chunfu; Vitek, Charles; Liulchuk, Maria; Nizova, Natalya

    2015-01-01

    An accurate accessible test for early infant diagnosis (EID) is crucial for identifying HIV-infected infants and linking them to treatment. To improve EID services in Ukraine, dried blood spot (DBS) samples obtained from 237 HIV-exposed children (≤18 months of age) in six regions in Ukraine in 2012 to 2013 were tested with the AmpliSens DNA-HIV-FRT assay, the Roche COBAS AmpliPrep/COBAS TaqMan (CAP/CTM) HIV-1 Qual test, and the Abbott RealTime HIV-1 Qualitative assay. In comparison with the paired whole-blood results generated from AmpliSens testing at the oblast HIV reference laboratories in Ukraine, the sensitivity was 0.99 (95% confidence interval [CI], 0.95 to 1.00) for the AmpliSens and Roche CAP/CTM Qual assays and 0.96 (95% CI, 0.90 to 0.98) for the Abbott Qualitative assay. The specificity was 1.00 (95% CI, 0.97 to 1.00) for the AmpliSens and Abbott Qualitative assays and 0.99 (95% CI, 0.96 to 1.00) for the Roche CAP/CTM Qual assay. McNemar analysis indicated that the proportions of positive results for the tests were not significantly different (P > 0.05). Cohen's kappa (0.97 to 0.99) indicated almost perfect agreement among the three tests. These results indicated that the AmpliSens DBS and whole-blood tests performed equally well and were comparable to the two commercially available EID tests. More importantly, the performance characteristics of the AmpliSens DBS test meets the World Health Organization EID test requirements; implementing AmpliSens DBS testing might improve EID services in resource-limited settings. PMID:26447114

  2. Tidal stripping of stars near supermassive black holes

    Directory of Open Access Journals (Sweden)

    Blandford R.

    2012-12-01

    Full Text Available In a binary system composed of a supermassive black hole and a star orbiting the hole in an equatorial, circular orbit, the stellar orbit will shrink due to the action of gravitational radiation, until the star fills its Roche lobe outside the Innermost Stable Circular Orbit (ISCO of the hole or plunges into the hole. In the former case, gas will flow through the inner Lagrange point (L1 to the hole. If this tidal stripping process happens on a time scale faster than the thermal time scale but slower than the dynamical time scale, the entropy as a function of the interior mass is conserved. The star will evolve adiabatically, and, in most cases, will recede from the hole while filling its Roche lobe. We calculate how the stellar equilibrium properties change, which determines how the stellar orbital period and mass-transfer rate change through the “Roche evolution” for various types of stars in the relativistic regime. We envisage that the mass stream eventually hits the accretion disc, where it forms a hot spot orbiting the hole and may ultimately modulate the luminosity with the stellar orbital frequency. The ultimate goal is to probe the mass and spin of the hole and provide a test of general relativity in the strong-field regime from the resultant quasi-periodic signals. The observability of such a modulation is discussed along with a possible interpretation of an intermittent 1 hour period in the X-ray emission of RE J1034+ 396.

  3. Asian Highlands Perspectives 10

    Directory of Open Access Journals (Sweden)

    various

    2011-12-01

    Full Text Available The third volume of collected essays from AHP includes ten new articles, one essay in the Mongghul language, three original short stories, and five folktales. The A mdo Tibetan Lab rtse Ritual by Kelsang Norbu Childbirth and Childcare in Rdo sbis Tibetan Township by Klu mo tshe ring and Gerald Roche Dmu rdo: A Powerful Hero and Mountain Deity by G.yung 'brug and Rin chen rdo rje Echoes from Si gang lih: Burao Yilu's 'Moon Mountain'by Mark Bender The Failure of Vocational Training in Tibetan Areas of China by Shiyong, Wang Fuel and Solar Cooker Impact in Ya na gdung Village, Gcan tsha County, Mtsho sngon (Qinghai Province by Rdo rje don 'grub "I, Ya ri a bsod, Am a Dog": The Life and Music of a Tibetan Mendicant Singer by Skal dbang skyid, Sha bo don sgrub rdo rje, Sgrol ma mtsho, Gerald Roche, Eric Schweickert, and Dpa' rtse rgyal Purity and Fortune in Phug sde Village Rituals by Sa mtsho skyid and Gerald Roche Rgyas bzang Tibetan Tribe Hunting Loreby Bkra shis dpal 'bar sa.bə: A Tibetan Rite of Passageby Lhundrom Muulasan Mongghulby Limusishiden Story - Fateby Gelsang Lhamu Story - A Stolen Journey by Blo bzang tshe ring Story - Is It Karma?by Pad ma rgya mtsho Folklore - Bear and Rabbit (Iby G.yu lha Folklore - Bear and Rabbit (IIby Snying dkar skyid Folklore - The Frog Boy and His Family by Chodpaylhamo Folklore - Mchig nges and Repaying a Debt of Gratitude by Zla ba sgrol ma

  4. Asian Highlands Perspectives 1

    Directory of Open Access Journals (Sweden)

    Various

    2009-03-01

    Full Text Available •\tA mdo Tibetan Tongue Twisters (7-51 Authors: Blo rtan rdo rje, Charles Kevin Stuart, and Gerald Roche •\tAn Amdo Tibetan Village New Year Trance Medium Ritual (53-64 Authors: Dpal ldan bkra shis, and Charles Kevin Stuart •\tCalling Back the Lost Namuyi Tibetan Soul (65-115 Authors: Libu Lakhi, Charles Kevin Stuart, and Gerald Roche •\tDying Hunters, Poison Plants, and Mute Slaves-Nature and Tradition in Contemporary Nuosu Yi Poetry (117-158 Author: Bender, Mark •\tThe Ersu Shaba Pictographic Writing System (159-186 Author: Sun, Hongkai •\tThe Fourth Belmang-Bodhisattva, Estate Lord, Tibetan Militia Leader, and Chinese Government Official (187-211 Author: Nietupski, Paul K. •\tThe Horse With Two Saddles-Tamxhwe in Modern Golok (213-235 Author: Pirie, Fernanda •\tSeating, Money, and Food at an Amdo Village Funeral (237-294 Authors: Rin chen rdo rje, and Charles Kevin Stuart •\tThe Sengze Village Mani (295-312 Authors: Dkon mchog dge lugs, and Charles Kevin Stuart •\tTibetan Life and Tibetological Discourse-Differences and Recommendations (313-329 Author: Roche, Gerald, Nag tshang grub rgyal, and Mtsho mo skyid •\tA Response to Ways and the Syntax of Noun Phrases in Qinghai Chinese Dialects (331-347 Author: Dede, Keith •\tLazi (Lab rtse Construction in Karmatang (Skar ma thang Village (349-366 Author: Tshe mdo •\tReview-Wutun (367-371 Author: Slater, Keith •\tStory-God Door (373-382 Author: Tshe dpag rnam rgyal

  5. Body Mass Index in Pregnancy Does Not Affect Peroxisome Proliferator-activated Receptor Gamma Promoter Region (−359 to −260) Methylation in the Neonate

    Science.gov (United States)

    Casamadrid, VRE; Amaya, CA; Mendieta, ZH

    2016-01-01

    Background: Obesity in pregnancy can contribute to epigenetic changes. Aim: To assess whether body mass index (BMI) in pregnancy is associated with changes in the methylation of the peroxisome proliferator-activated receptor γ (PPAR) promoter region (-359 to - 260) in maternal and neonatal leukocytes. Subjects and Methods: In this matched, cohort study 41 pregnant women were allocated into two groups: (a) Normal weight (n = 21) and (b) overweight (n = 20). DNA was extracted from maternal and neonatal leukocytes (4000-10,000 cells) in MagNA Pure (Roche) using MagNA Pure LC DNA Isolation Kit 1 (Roche, Germany). Treatment of DNA (2 μg) was performed with sodium bisulfite (EZ DNA Methylation-Direct™ Kit; Zymo Research). Real-time quantitative polymerase chain reaction (qPCR) was performed in a LightCycler 2.0 (Roche) using the SYBR® Advantage® qPCR Premix Kit (Clontech). The primers used for PPARγ coactivator (PPARG) M3 were 5’- aagacggtttggtcgatc-3’ (forward), and5’- cgaaaaaaaatccgaaatttaa-3’ (reverse) and those for PPARG unmethylated were: 5’-gggaagatggtttggttgatt-3’ (forward) and 5’- ttccaaaaaaaaatccaaaatttaa-3’ (reverse). Intergroup differences were calculated using the Mann-Whitney U-test, and intragroup differences, with the Wilcoxon test (IBM SPSS Statistics for Windows, Version 19.0. Armonk, NY: IBM Corp.). Results: Significant differences were found in BMI, pregestational weight, and postdelivery weight between groups but not in the methylation status of the PPARγ promoter region (-359 to - 260). Conclusion: The PPARγ promoter region (-359 to - 260) in peripheral leukocytes is unlikely to get an obesity-induced methylation in pregnancy. PMID:27144075

  6. 蛋白芯片检测肿瘤标志物及其与其他方法的比较

    Institute of Scientific and Technical Information of China (English)

    娄加陶; 顾军; 高春芳; 范列英; 赵虎; 李智; 沈霞; 孔宪涛; 仲人前

    2009-01-01

    目的 研究多肿瘤标志物蛋白芯片检测系统(C-12)检测肿瘤标志物的性能及其与国外检测系统的比较.方法 分别选用湖州数康生物科技有限公司C-12、Roche Modular E170、Elecsys 2010和Abbott 12000 4种检测系统对甲胎蛋白(AFP)、癌胚抗原(12EA)、前列腺特异性抗原(PSA)、游离PSA(f-PSA)、糖类抗原125(CA125)、糖类抗原153(CA153)、神经元特异性烯醇化酶(NSE)、糖类抗原242(CA242)、人绒毛膜促性腺激素(β-HCG)和糖类抗原199(CA199)临床常见肿瘤标志物进行检测,所测得的数据进行统计学分析.结果 C-12与Roche Modular E170、Eleesys 2010和Abbott 12000 4种检测系统在肿瘤标志物的检测中符合率呈显著正相关,而且敏感性、特异性、阳性预测值、阴性预测值等均有很高的一致性.结论C-12与Roche Modular E170、Eleesys 2010和Abbou 12000在检测肿瘤标志物中具有较高的一致性,对肿瘤标志物的检测具有较高的实用价值.

  7. Performance of an Early Infant Diagnostic Test, AmpliSens DNA-HIV-FRT, Using Dried Blood Spots Collected from Children Born to Human Immunodeficiency Virus-Infected Mothers in Ukraine.

    Science.gov (United States)

    Chang, Joy; Tarasova, Tetyana; Shanmugam, Vedapuri; Azarskova, Marianna; Nguyen, Shon; Hurlston, Mackenzie; Sabatier, Jennifer; Zhang, Guoqing; Osmanov, Saladin; Ellenberger, Dennis; Yang, Chunfu; Vitek, Charles; Liulchuk, Maria; Nizova, Natalya

    2015-12-01

    An accurate accessible test for early infant diagnosis (EID) is crucial for identifying HIV-infected infants and linking them to treatment. To improve EID services in Ukraine, dried blood spot (DBS) samples obtained from 237 HIV-exposed children (≤18 months of age) in six regions in Ukraine in 2012 to 2013 were tested with the AmpliSens DNA-HIV-FRT assay, the Roche COBAS AmpliPrep/COBAS TaqMan (CAP/CTM) HIV-1 Qual test, and the Abbott RealTime HIV-1 Qualitative assay. In comparison with the paired whole-blood results generated from AmpliSens testing at the oblast HIV reference laboratories in Ukraine, the sensitivity was 0.99 (95% confidence interval [CI], 0.95 to 1.00) for the AmpliSens and Roche CAP/CTM Qual assays and 0.96 (95% CI, 0.90 to 0.98) for the Abbott Qualitative assay. The specificity was 1.00 (95% CI, 0.97 to 1.00) for the AmpliSens and Abbott Qualitative assays and 0.99 (95% CI, 0.96 to 1.00) for the Roche CAP/CTM Qual assay. McNemar analysis indicated that the proportions of positive results for the tests were not significantly different (P > 0.05). Cohen's kappa (0.97 to 0.99) indicated almost perfect agreement among the three tests. These results indicated that the AmpliSens DBS and whole-blood tests performed equally well and were comparable to the two commercially available EID tests. More importantly, the performance characteristics of the AmpliSens DBS test meets the World Health Organization EID test requirements; implementing AmpliSens DBS testing might improve EID services in resource-limited settings. PMID:26447114

  8. Crystallization-induced dynamic resolution R-epimer from 25-OCH3-PPD epimeric mixture.

    Science.gov (United States)

    Zhang, Sainan; Tang, Yun; Cao, Jiaqing; Zhao, Chen; Zhao, Yuqing

    2015-11-15

    25-OCH3-PPD is a promising antitumor dammarane sapogenin isolated from the total saponin-hydrolyzed extract of Panax ginseng berry and Panax notoginseng leaves. 20(R)-25-OCH3-PPD was more potent as an anti-cancer agent than 20(S)-25-OCH3-PPD and epimeric mixture of 25-OCH3-PPD. This paper describes the rapid separation process of the R-epimer of 25-OCH3-PPD from its epimeric mixture by crystallization-induced dynamic resolution (CIDR). The optimized CIDR process was based on single factor analysis and nine well-planned orthogonal design experiments (OA9 matrix). A rapid and sensitive reverse phase high-performance liquid chromatographic (HPLC) method with evaporative light-scattering detector (ELSD) was developed and validated for the quantitation of 25-OCH3-PPD epimeric mixture and crystalline product. Separation and quantitation were achieved with a silica column using a mobile phase consisting of methanol and water (87:13, v/v) at a flow rate of 1.0mL/min. The ELSD detection was performed at 50°C and 3L/min. Under conditions involving 3mL of 95% ethanol, 8% HCl, and a hermetically sealed environment for 72h, the maximum production of 25(R)-OCH3-PPD was achieved with a chemical purity of 97% and a total yield of 87% through the CIDR process. The 25(R)-OCH3-PPD was nearly completely separated from the 220mg 25-OCH3-PPD epimeric mixture. Overall, a simple and steady small-batch purification process for the large-scale production of 25(R)-OCH3-PPD from 25-OCH3-PPD epimeric mixture was developed.

  9. Uranium and thorium in Cenozoic basaltods of Kamchatka

    International Nuclear Information System (INIS)

    Regularities in distribution of radioactive elements (RAE) in basaltoids of Kamchatka have been analyzed. The RAE concentration in samples was determined by γ-spectrometric method. The results compared with the instrumental neutron-activation analysis data are found to be in agreement. Results of evaluating the average contents of U, Th and roch-forming elements in ce-- nozoic basaltoids are presented. The radiogeochemical data enable to associate the origin of the Kamchatka Cenozoic basaltoids with both fractional melting of the upper mantle depleted of radioactive elements and the development of magmatic chambers in submerged blocks of the Pre-Cretaceous melanocratic basement the composition of which is close to oceanic tholeiite

  10. Active and realistic passive marijuana exposure tested by three immunoassays and GC/MS in urine

    Energy Technology Data Exchange (ETDEWEB)

    Mule, S.J.; Lomax, P.; Gross, S.J.

    1988-05-01

    Human urine samples obtained before and after active and passive exposure to marijuana were analyzed by immune kits (Roche, Amersham, and Syva) and gas chromatography/mass spectrometry (GC/MS). Seven of eight subjects were positive for the entire five-day test period with one immune kit. The latter correlated with GC/MS in 98% of the samples. Passive inhalation experiments under conditions likely to reflect realistic exposure resulted consistently in less than 10 ng/mL of cannabinoids. The 10-100-ng/mL cannabinoid concentration range essential for detection of occasional and moderate marijuana users is thus unaffected by realistic passive inhalation.

  11. 单克隆抗体Tocilizumab获准用于治疗活动期全身型幼年特发性关节炎

    Institute of Scientific and Technical Information of China (English)

    2011-01-01

    @@ 由罗氏(Roche)旗下基因泰克(Genentech)公司开发的单克隆抗体tocilizumab(商品名为Aetemra)于近期获FDA批准,用于治疗活动期全身型幼年特发性关节炎(systemic juvenile idiopathic arthritis,SJIA),适用年龄为2岁及2岁以上,可单独使用,也可与甲氨蝶呤合用.SJIA也称斯蒂尔病(Still's dis-ease),为一种罕见的可威胁生命的疾病.

  12. Private visit to the CMS assembly site of Dr. Fidel Castro Diaz-Balart from the Superior Institute of Sciences and Nuclear Technologies, Havana, accompanied by His Excellency Mr. Emilio Caballero, Ambassador, Permanent Mission of Cuba in Paris.

    CERN Multimedia

    Maximilien Brice

    2002-01-01

    Photo 01: Left to right: His Excellency Mr Emilio Caballero; Prof. Tejinder Virdee, Deputy Spokesman of the CMS experiment; Dr Fidel Castro Diaz-Balart; Dr Matthias Schroeder, physicist, Experimental Physics division; Mrs Noëlle Levy, Casa del Habano, Geneva; Dr John Ellis, Adviser for Non-Member State relations; Dr Christian Roche, Senior Advisor to the Director-General. Photo 02: Left to right: His Excellency Mr Emilio Caballero, Prof. Tejinder Virdee, Dr Fidel Castro Diaz-Balart; Dr Matthias Schroeder; Mrs Noëlle Levy, Prof. Juan Antonio Rubio, Head of the Education and Technology Transfer division; Dr John Ellis.

  13. Rotational mixing in close binaries

    CERN Document Server

    de Mink, S E; Langer, N; Yoon, S -Ch; Brott, I; Glebbeek, E; Verkoulen, M; Pols, O R

    2008-01-01

    Rotational mixing is a very important but uncertain process in the evolution of massive stars. We propose to use close binaries to test its efficiency. Based on rotating single stellar models we predict nitrogen surface enhancements for tidally locked binaries. Furthermore we demonstrate the possibility of a new evolutionary scenario for very massive (M > 40 solar mass) close (P < 3 days) binaries: Case M, in which mixing is so efficient that the stars evolve quasi-chemically homogeneously, stay compact and avoid any Roche-lobe overflow, leading to very close (double) WR binaries.

  14. 让你的家也穿上GAULITIER

    Institute of Scientific and Technical Information of China (English)

    2010-01-01

    随着各种跨界合作的不断升级,时尚和室内装饰的联姻也就顺水推舟,牵起手来,法国著名家居品牌Roche Bobois和Jean Paul Gaultier的跨界合作成为法国室内设计50周年庆典的一件大事,Gaultier的经典风格和图案被用在床品、沙发、镜子等家居用品上。

  15. Continuum viscoplastic simulation of a granular column collapse on large slopes : μ(I) rheology and lateral wall effects

    Science.gov (United States)

    Martin, Nathan; Mangeney, Anne; Ionescu, Ioan; Bouchut, Francois

    2016-04-01

    The description of the mechanical behaviour of granular flows and in particular of the static/flowing transition is still an open and challenging issue with strong implication for hazard assessment [{Delannay et al.}, 2016]. In particular, {detailed quantitative} comparison between numerical models and observations is necessary to go further in this direction. We simulate here dry granular flows resulting from the collapse of granular columns on an inclined channel (from horizontal to 22^o) and compare precisely the results with laboratory experiments performed by {Mangeney et al.} [2010] and {Farin et al.} [2014]. Incompressibility is assumed despite the dilatancy observed in the experiments (up to 10%). The 2-D model is based on the so-called μ(I) rheology that induces a Drucker-Prager yield stress and a variable viscosity. A nonlinear Coulomb friction term, representing the friction on the lateral walls of the channel is added to the model. We demonstrate that this term is crucial to accurately reproduce granular collapses on slopes higher than 10o whereas it remains of little effect on horizontal slope [{Martin et al.}, 2016]. We show that the use of a variable or a constant viscosity does not change significantly the results provided that these viscosities are of the same order [{Ionescu et al.}, 2015]. However, only a fine tuning of the constant viscosity (η = 1 Pa.s) makes it possible to predict the slow propagation phase observed experimentally on large slopes. This was not possible when using, without tuning, the variable viscosity calculated from the μ(I) rheology with the parameters estimated from experiments. Finally, we discuss the well-posedness of the model with variable and constant viscosity based in particular on the development of shear bands observed in the numerical simulations. References Delannay, R., Valance, A., Mangeney, A., Roche, O., and Richard, P., 2016. Granular and particle-laden flows: from laboratory experiments to field

  16. Differentiation of Mature and Immature Vasculature

    OpenAIRE

    sprotocols

    2014-01-01

    ### Materials Needed 1. PAP pen (Abcam ab2601) - Histochoice MB Fixative (Electron Microscopy Sciences 64115-01) - 10xPBS (Fisher BP3994) - Triton X (Roche 12754423) - Bovine serum albumin (Sigma A8022-500G) - Mouse CD31 [PECAM] (BD Biosciences 555025) - Rabbit alpha smooth muscle actin (Abcam ab5694) - Alexa Fluor 555 donkey anti-mouse IgG (H L) (Invitrogen A31570) - Alexa Fluor 488 donkey anti-rabbit IgG (H L) (Invitrogen A21206) ### Solutions 1. Wash Bu...

  17. Photometry and Spectroscopy of the Optical Companion to the Pulsar PSR J1740-5340 in the Globular Cluster NGC 6397

    OpenAIRE

    Kaluzny, J.; Rucinski, S. M.; Thompson, I. B.

    2002-01-01

    We present photometric and spectroscopic observations of the optical companion to the millisecond radio pulsar PSR J1740-5340 in the globular cluster NGC 6397. An analysis of the photometric variability in the B, V, and I-bands indicates an inclination of the system of 43.9+-2.1 degrees if the optical companion fills its Roche lobe (a semi-detached configuration). The spectroscopic data show a radial velocity variation with a semi-amplitude of K=137.2 +- 2.4 km/sec, and a system velocity gamm...

  18. Interacting binary stars

    CERN Document Server

    Sahade, Jorge; Ter Haar, D

    1978-01-01

    Interacting Binary Stars deals with the development, ideas, and problems in the study of interacting binary stars. The book consolidates the information that is scattered over many publications and papers and gives an account of important discoveries with relevant historical background. Chapters are devoted to the presentation and discussion of the different facets of the field, such as historical account of the development in the field of study of binary stars; the Roche equipotential surfaces; methods and techniques in space astronomy; and enumeration of binary star systems that are studied

  19. The application of adenosine triphosphate bioluminescence assay in the diagnosis of multidrug-resistant ;Mycobacterium tuberculosis%三磷酸腺苷发光法在耐多药结核分枝杆菌诊断中的运用

    Institute of Scientific and Technical Information of China (English)

    刘君; 胡嘉波; 裴豪; 蒯守刚; 陈丽艳

    2013-01-01

    目的:通过与罗氏固体培养法比较,评估三磷酸腺苷发光法检测耐多药结核分枝杆菌的可行性。方法采用三磷酸腺苷发光法与罗氏固体培养法同时检测和分析149例临床分离的结核分枝杆菌。结果三磷酸腺苷发光法与罗氏固体培养法的一致率为92.6%(138/149),差异无统计学意义(χ2=0.57,P=0.45)。三磷酸腺苷发光法检测时间为(6.6±2.1)d,明显快于传统罗氏固体培养法的28 d(t=422.7,P<0.001)。结论与常规检测方法比较,三磷酸腺苷发光法具有快速、简便、准确性高等优点,对耐多药结核患者的早期诊断和耐药结核分枝杆菌流行的控制有很大帮助,适合实验室开展。%Objective To compare with Roche solid culture method,and to evaluate the feasibility of adenosine triphosphate bioluminescence assay for detecting multidrug-resistant Mycobacterium tuberculosis.Methods By Roche solid culture method and adenosine triphosphate bioluminescence assay,149 clinical isolates of Mycobacterium tuber-culosis were determined and analyzed.Results The coincidence rate of adenosine triphosphate bioluminescence assay with Roche solid culture method was 92.6%(138/149),and the difference had no statistical significance (χ2 =0.57, P=0.45).The detection time of adenosine triphosphate bioluminescence assay was (6.6 ±2.1)d,which was faster than that of Roche solid culture method (28 d,t=422.7,P<0.001).Conclusions Compared with the conventional detection methods,adenosine triphosphate bioluminescence assay is simple,rapid and accurate.It is helpful for detecting multidrug-resistant tuberculosis patients and controlling the prevalence of Mycobacterium tuberculosis.It is suitable for clinical laboratory.

  20. A fast and robust method for full genome sequencing of Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) Type 1 and Type 2

    DEFF Research Database (Denmark)

    Kvisgaard, Lise Kirstine; Hjulsager, Charlotte Kristiane; Fahnøe, Ulrik;

    2013-01-01

    PRRSV is a positive-sense RNA virus with a high degree of genetic variability among isolates. For diagnostic sensitivity and vaccine design it is essential to monitor PRRSV genetic diversity. However, to date only a few full genome sequences of PRRSV isolates have been made publicly available. In...... viruses and the protocols performed well on all three NGS platforms tested (Roche 454 FLX, Illumina HiSeq2000, and Ion Torrent PGM™ Sequencer). These methods will greatly facilitate the generation of more full genome PRRSV sequences globally....

  1. Development of an oral dosage form of capecitabine with modified release characteristics

    OpenAIRE

    Meulenaar, J

    2013-01-01

    Capecitabine is an orally-administered chemotherapeutic agent used in the treatment of a.o. metastatic breast, gastric and colorectal cancer and is commercially available as an immediate release tablet (Xeloda®, Roche). Capecitabine is a pre-pro-drug that is enzymatically converted into 5-fluorouracil (5-FU) in the liver and the tumor. In tumor it inhibits DNA synthesis and slows tumor growth. To form 5-FU, the activation of capecitabine follows a three-step enzymatic pathway with two interme...

  2. Effects of rearing density on seabass (Dicentrarchus labrax, L. gene expression

    Directory of Open Access Journals (Sweden)

    M. Saroglia

    2011-03-01

    Full Text Available Animal welfare involves the subjective feeling of animals and the experience of pleasure, pain, frustration, hunger or other states and it is therefore difficult to define and to measure (Drawkins, 1990. When water quality criteria are respected and feeding restrictions are avoided, fish density may become one of the more relevant factors expected to potentially affect fish welfare. Such high density environment, may lead to a fish conditions that cannot be described or forecast simply with measurement of hematological responses (Roche and Bogé, 1996, in the same time, detection of zootechnical performances, do not provide a specific indications...

  3. Active and realistic passive marijuana exposure tested by three immunoassays and GC/MS in urine

    International Nuclear Information System (INIS)

    Human urine samples obtained before and after active and passive exposure to marijuana were analyzed by immune kits (Roche, Amersham, and Syva) and gas chromatography/mass spectrometry (GC/MS). Seven of eight subjects were positive for the entire five-day test period with one immune kit. The latter correlated with GC/MS in 98% of the samples. Passive inhalation experiments under conditions likely to reflect realistic exposure resulted consistently in less than 10 ng/mL of cannabinoids. The 10-100-ng/mL cannabinoid concentration range essential for detection of occasional and moderate marijuana users is thus unaffected by realistic passive inhalation

  4. The Witten-Reshetikhin-Turaev invariants of finite order mapping tori I

    DEFF Research Database (Denmark)

    Ellegaard Andersen, Jørgen

    We formulate the Asymptotic Expansion Conjecture for the Witten-Reshetikhin-Turaev quantum invariants of closed oriented three manifolds. For finite order mapping tori, we study these quantum invariants via the geometric gauge theory approach to the corresponding quantum representations and prove......, using a version of the Lefschetz-Riemann-Roch Theorem due to Baum, Fulton, MacPherson and Quart, that the quantum invariants can be expressed as a sum over the components of the moduli space of flat connections on the mapping torus. Moreover, we show that the term corresponding to a component...

  5. Massively parallel pyrosequencing of the mitochondrial genome with the 454 methodology in forensic genetics

    DEFF Research Database (Denmark)

    Mikkelsen, Martin; Frank-Hansen, Rune; Hansen, Anders Johannes;

    2014-01-01

    RESULTS: of sequencing of whole mitochondrial genome, HV1 and HV2 DNA with the second generation system (SGS) Roche 454 GS Junior were compared with results of Sanger sequencing and SNP typing with SNaPshot single base extension detected with MALDI-TOF and capillary electrophoresis. We investigated...... in homopolymers up to 4 bases, and up to 6 bases could be sequenced with similar success if the results were carefully, visually inspected. The 454 technology was able to detect mixtures or heteroplasmy of approximately 10%. We detected previously unreported heteroplasmy in the GM9947A component of the NIST human...

  6. Development of a cost-effective metabarcoding strategy for analysis of the marine phytoplankton community

    OpenAIRE

    Yoon, Tae-Ho; Kang, Hye-Eun; Kang, Chang-Keun; Lee, Sang Heon; Ahn, Do-Hwan; Park, Hyun; Kim, Hyun-Woo

    2016-01-01

    We developed a cost-effective metabarcoding strategy to analyze phytoplankton community structure using the Illumina MiSeq system. The amplicons (404–411 bp) obtained by end-pairing of two reads were sufficiently long to distinguish algal species and provided barcode data equivalent to those generated with the Roche 454 system, but at less than 1/20th of the cost. The original universal primer sequences targeting the 23S rDNA region and the PCR strategy were both modified, and this resulted i...

  7. Long-term photometric and spectroscopic observations of the near-contact binary KR Cygni

    OpenAIRE

    Sipahi, E.; Cakirli, O.; Ibanoglu, C.

    2012-01-01

    We present the multi-color, five-year light curves and the first radial velocities of the near-contact binary system KR Cyg. We derived the masses of the components as 2.88$\\pm$0.20 M$_{\\odot}$ and 1.26$\\pm$0.07 M$_{\\odot}$ and the radii as 2.59$\\pm$0.06 R$_{\\odot}$ and 1.80$\\pm$0.04 R$_{\\odot}$. Analyses of the UBVR light curves and the radial velocities indicate that none of the components exactly fill their corresponding Roche lobes. We have calculated the distance to the system of KR Cyg ...

  8. Reading the Body in Bret Easton Ellis's American Psycho (1991) : Confusing Signs and Signifiers

    OpenAIRE

    Roche, David

    2009-01-01

    http://www.graat.fr/roche.pdf International audience This article examines how matter, in Ellis's scandalous novel, is read according to the texts that inform the yuppie's etiquette, labeling the body as either “healthy” or “unhealthy.” Basing his argumentation on the works of Judith Butler, Luce Irigaray, Jacques Lacan, Clément Rosset and D.W. Winnicott, the author argues that the body is perceived as a sign of something when it is actually an effect of the signifier, and that the nove...

  9. AM Canum Venaticorum Progenitors with Helium Star Donors and the resultant Explosions

    OpenAIRE

    Brooks, Jared; Bildsten, Lars; Marchant, Pablo; Paxton, Bill

    2015-01-01

    We explore the outcome of mass transfer via Roche lobe overflow (RLOF) of $M_{\\rm He}\\lesssim0.51 M_\\odot$ pure helium burning stars in close binaries with white dwarfs (WDs). The evolution is driven by the loss of angular momentum through gravitational wave radiation (GWR), and both stars are modeled using Modules for Experiments in Stellar Astrophysics (MESA). The donors have masses of $M_{\\rm He}=0.35, 0.4, \\&\\ 0.51M_\\odot$ and accrete onto WDs of mass $M_{\\rm WD}$ from $0.6M_\\odot$ to $1....

  10. CEA, CYFRA 21-1, NSE, and ProGRP in the diagnosis of lung cancer: a multivariate approach

    OpenAIRE

    Gruber, Christine; Hatz, Rudolf; Reinmiedl, Judith; Nagel, Dorothea; Stieber, Petra

    2008-01-01

    We retrospectively studied the single and combined diagnostic value of carcinoembryonic antigen (CEA), cytokeratin fragment 19 (CYFRA 21-1), neuron specific enolase (NSE) and pro-gastrin-releasing peptide (ProGRP), which were routinely analysed in patients with lung tumours of unknown origin at the time of admission to hospital. Inclusion criteria were the determination of CEA (AxSYM/Abbott), CYFRA 21-1 (ElecSys/Roche) and NSE (Kryptor/Brahms). We examined 1747 patients, where 1325 suffered f...

  11. Comparison of Lumipulse® G1200 with Kryptor and Modular E170 for the measurement of 7 tumor markers

    OpenAIRE

    Marlet, Julien; Bernard, Maguy

    2014-01-01

    Background: Tumor marker measurements are becoming essential for prognosis and follow-up of patients in oncology. In this context, we aimed to compare a new analyzer, Lumipulse G1200 (Fujirebio group, distributed in Europe by the Innogenetics group) with Kryptor (Thermo Fisher Scientific B.R.A.H.M.S, Asnières, France) and Modular Elecsys E170 (Roche Diagnostics, Meylan, France) for the measurement of seven tumor markers: PSA, AFP, CEA, CA 15-3, CA 125, CA 19-9 and Cyfra 21-1.Methods: 471 seru...

  12. Blood Sugar Values and Intestine Mucosa Integrity in Suckling Pigs in Relation to their Nutritional Status

    OpenAIRE

    Olga Rada; Horea Sărăndan; Liliana Vasile; Diana Argherie

    2010-01-01

    The experiment included 106 healthy piglets aged 1-14 days and 38 piglets suffering from diarrhoea, aged 1-7 days. The blood sugar was measured with the help of Accu-chek Go (Roche Diagnostics GMBH, Germany) halfway between two sucklings. Segments with duodenum, jejunum and ileum were harvested from the diarrhoea suffering piglets and coloured HE preparations were examined under a stereoscopic magnifying glass and the microscope. With healthy piglets, the average blood sugar was of 61.55±10.2...

  13. Strategic Planning and Performance Management Develop and Measure a Winning Strategy

    CERN Document Server

    Kenny, Graham

    2005-01-01

    Strategic Planning and Performance Measurement: Develop & Measure a Winning Strategy, provides a clear and concise roadmap for designing, implementing and measuring strategy. The focus is on strategic factors, which are defined in a unique way as the criteria on which an organization or business unit has to do well in order to succeed. For organizations to be successful, they must take a stakeholder perspective of their performance - stakeholders such as customers, suppliers, employees and owners. The book cites many case studies including: 7-Eleven, Roche, K-mart, McDonald's, Levi Strauss,

  14. Les sols, richesses cachées de la planète

    OpenAIRE

    Garré, Sarah; Carnol, Monique; Cornelis, Jean-Thomas

    2015-01-01

    Toute forme de vie sur terre doit beaucoup aux sols. Aussi discrets que dynamiques, vitaux que complexes, les sols sont des réacteurs bio-physico-chimiques, situés à l’interface entre les roches, la végétation, l’air et l’eau. L’étude de cette ressource non renouvelable, soumise à des pressions croissantes, requiert une approche interdisciplinaire, indispensable pour une gestion raisonnée et durable des écosystèmes. Comment sont définis les sols, comment les étudions-nous, comment aborder leu...

  15. Gravitational waves emitted by solar-type stars excited by orbiting planets

    CERN Document Server

    Berti, E; Berti, Emanuele; Ferrari, Valeria

    2001-01-01

    The possibility of exciting the g-modes of a solar-type star as a consequence of the gravitational interaction with a close companion (a planet or a brown dwarf) is studied by a perturbative approach. The amplitude of the emitted gravitational wave is computed and compared with the quadrupole emission of the system, showing that in some cases it can be considerably larger. The effects of radiation reaction are considered to evaluate the timescale of the emission process, and a Roche lobe analysis is used to establish the region where the companion can orbit without being disrupted by tidal interactions with the star.

  16. 5-type HPV mRNA versus 14-type HPV DNA test: test performance, over-diagnosis and overtreatment in triage of women with minor cervical lesions

    OpenAIRE

    Westre, Bjørn; Giske, Anita; Guttormsen, Hilde; Sørbye, Sveinung Wergeland; Skjeldestad, Finn Egil

    2016-01-01

    Background Repeat cytology and HPV testing is used in triage of women with minor cytological lesions. The objective of this study was to evaluate 14-type HPV DNA and 5-type HPV mRNA testing in delayed triage of women with ASC-US/LSIL. Methods We compared a DNA test (Roche Cobas 4800) and an 5-type mRNA test (PreTect HPV-Proofer). In total 564 women were included in the study. Results The sensitivity among solved cases for CIN3+ were 100 % (15/15) for both tests. The sensitivity for CIN2+ of t...

  17. The evolution of naked helium stars with a neutron-star companion in close binary systems

    OpenAIRE

    Dewi, J D M; Pols, O. R; Savonije, G.J.; Heuvel, E.P.J. van den

    2002-01-01

    The evolution of helium stars with masses of 1.5 - 6.7 M_sun in binary systems with a 1.4 M_sun neutron-star companion is presented. Such systems are assumed to be the remnants of Be/X-ray binaries with B-star masses in the range of 8 - 20 M_sun which underwent a case B or case C mass transfer and survived the common-envelope and spiral-in process. The orbital period is chosen such that the helium star fills its Roche lobe before the ignition of carbon in the centre. We distinguish case BA (i...

  18. Advances in astronomy and astrophysics 9

    CERN Document Server

    Kopal, Zdenek

    1972-01-01

    Advances in Astronomy and Astrophysics, Volume 9 covers reviews on the advances in astronomy and astrophysics. The book presents reviews on the Roche model and its applications to close binary systems. The text then describes the part played by lunar eclipses in the evolution of astronomy; the classical theory of lunar eclipses; deviations from geometrical theory; and the methods of photometric observations of eclipses. The problems of other phenomena related in one way or another to lunar eclipses are also considered. The book further tackles the infrared observation on the eclipsed moon, as

  19. Rituximab可用于治疗慢性淋巴细胞白血病

    Institute of Scientific and Technical Information of China (English)

    2010-01-01

    @@ 罗氏(Roche)公司与生物基因艾迪克(Biogen Idec)公司联合开发的抗CD20药物Rituxan/MabThera(通用名:利妥昔单抗),近期已获美国FDA批准,与化疗联合用于初发或已经接受过治疗(复发性或顽固性)的慢性淋巴细胞白血病(chronic lymphocytic leukaemia,CLL)患者.

  20. Estudio de bioequivalencia de clonazepam, tabletas de 2 mg, en voluntarios sanos colombianos Bioequivalence study of clonazepam 2 mg tablets in colombian healthy volunteers

    OpenAIRE

    Victoria Eugenia Toro Pareja; Juan Carlos Ríos Toro; Blanca Cecilia Montoya Beltrán; Lina María Peña Acevedo; Margarita María Restrepo Garay; Sergio Parra; Rosendo Ricardo Archbold Joseph; Gloria Holguín Martínez; Fanny Cuesta González; Ómar de Jesús Correa Cano; Adriana María Ruiz Correa

    2007-01-01

    Con el fin de determinar la bioequivalencia de dos formulaciones de tabletas de 2 mg de clonazepam: Sedatril®/Clonazepam MK (Tecnoquímicas S. A., Cali, Colombia) como producto de prueba y Rivotril® (Roche Químicos e Farmacéuticas S. A., Río de Janeiro, Brasil), como producto de referencia, se realizó un estudio de bioequivalencia en 26 voluntarios sanos. Los productos de prueba y de referencia se administraron en condiciones de ayuno de acuerdo con un diseño cruzado aleatorio de dosis única, ...

  1. Comparison of the Reference Intervals Used for the Evaluation of Maternal Thyroid Function During Pregnancy Using Sequential and Nonsequential Methods

    Institute of Scientific and Technical Information of China (English)

    Jian-Xia Fan; Shuai Yang; Wei Qian; Feng-Tao Shi; He-Feng Huang

    2016-01-01

    Background:Maternal thyroid dysfunction is common during pregnancy,and physiological changes during pregnancy can lead to the overdiagnosis of hyperthyroidism and misdiagnosis of hypothyroidism with nongestation-specific reference intervals.Our aim was to compare sequential with nonsequential methods for the evaluation of thyroid function in pregnant women.Methods:We tested pregnant women who underwent their trimester prenatal screening at our hospital from February 2011 to September 2012 for serum thyroid stimulating hormone (TSH) and free thyroxine (FT4) using the Abbott and Roche kits.There were 447 and 200 patients enrolled in the nonsequential and sequential groups,respectively.The central 95% range between the 2.5th and the 97.5th percentiles was used as the reference interval for the thyroid function parameter.Results:The nonsequential group exhibited a significantly larger degree of dispersion in the TSH reference interval during the 2nd and 3rd trimesters as measured using both the Abbott and Roche kits (all P < 0.05).The TSH reference intervals were significantly larger in the nonsequential group than in the sequential group during the 3rd trimester as measured with both the Abbott (4.95 vs.3.77 mU/L,P < 0.001) and Roche kits (6.62 vs.5.01 mU/L,P =0.004).The nonsequential group had a significantly larger FT4 reference interval as measured with the Abbott kit during all trimesters (12.64 vs.5.82 pmol/L;7.96 vs.4.77 pmol/L;8.10 vs.4.77 pmol/L,respectively,all P < 0.05),whereas a significantly larger FT4 reference interval was only observed during the 24 trimester with the Roche kit (7.76 vs.5.52 pmol/L,P =0.002).Conclusions:It was more reasonable to establish reference intervals for the evaluation of maternal thyroid function using the sequential method during each trimester of pregnancy.Moreover,the exclusion of pregnancy-related complications should be considered in the inclusion criteria for thyroid function tests.

  2. How Zantac became the best-selling drug in history.

    Science.gov (United States)

    Wright, R

    1996-01-01

    Glaxo's Zantac began its dominance of the acid/peptic marketplace with a launch strategy, taking advantage of the established Roche sales force to rapidly promote the product. Educational symposia for physicians were instrumental in disseminating both disease and product information to primary care physicians and specialists. This technique not only pleased physicians (more referrals), but also increased public awareness of gastrointestinal disease, further expanding the patient market. Several novel marketing strategies contributed to Zantac's success, including the public-service announcements, celebrity media tours, and consumer-awareness bulletins, which brought the drug to the lay public and encouraged individuals to seek advice from their physicians. PMID:10169076

  3. Middle-to-Upper Palaeolithic site formation processes at the Bordes-Fitte rockshelter (Central France)

    DEFF Research Database (Denmark)

    Aubry, Thierry; Dimuccio, Luca Antonio; Buylaert, Jan-Pieter;

    2014-01-01

    . In this article we use the Middle and Early Upper Palaeolithic archaeo-stratigraphic record from the Bordes-Fitte rockshelter (les Roches d'Abilly site, Central France), a Bayesian analysis of the ages obtained by accelerator mass spectrometry radiocarbon on ultrafiltered collagen and by luminescence on quartz...... and feldspar grains, to establish a timeline for material culture and sedimentary dynamic changes during the Middle-to-Upper Palaeolithic transition. Technology, refitting studies and taphonomy of lithic artifacts recovered in the geoarchaeological field units D1 and D2 permit to characterize 3 reduction...

  4. 新魅东方

    Institute of Scientific and Technical Information of China (English)

    刘轶楠; 周静

    2009-01-01

    <正>Nao Mai书架(罗奇堡家居Roche- Bobois) Nao Mai系列将竹林的安静之美带入到生活之中,台面为纯竹条制成。设计师Stephane Lebrun将在亚洲旅行中得到的灵感运用在这款设计中,Nao Mai是泰语"竹子"的意思。此款书架有10种颜色可以选择。

  5. Collective Properties of X-ray Binary Populations of Galaxies III. The Low-mass X-ray Binary Luminosity Function

    OpenAIRE

    Bhadkamkar, Harshal; Ghosh, Pranab

    2013-01-01

    Continuing our exploration of the collective properties of low-mass X-ray binaries (LMXBs) in the stellar fields of normal galaxies, we compute in this paper the expected X-ray luminosity function (XLF) of LMXBs, starting from the results of the previous paper in the series (Paper II). We treat separately two classes of LMXB evolution, the first being close systems whose initial orbital periods are below the bifurcation period, wherein the companion is on the main sequence when Roche-lobe con...

  6. Foreign-branded Furniture Amid Crisis of Confidence

    Institute of Scientific and Technical Information of China (English)

    Zhu Zijun

    2011-01-01

    @@ "The clients raised questions about the place of origin of our furniture, and almost all the new orders have been cancelled or postponed since the Da Vinci controversy was reported," said Zhou Yufeng, China managing directorof the Roche Bobois (RB) Group, at a press conference held on July 20in Shanghai. His words showed his concerns of RB in China.However, RB isnot the only furniture firm to suffer from the Da Vinci scandal.Otherforeign brands are also facing a credibility crisis.

  7. Towards sequencing the 1 Gb wheat chromosome 3B

    OpenAIRE

    Choulet, Frédéric; Paux, Etienne; Leroy, Philippe; Sourdille, Pierre; Schlub, Stéphane; Brunel, Dominique; Barbe, Valérie; Wincker, Patrick; Feuillet, Catherine

    2009-01-01

    * BACKGROUND: Wheat is the most widely grown crop worldwide but so far, the access to its genome sequence has been hampered by its size (17 Gb) and highly repetitive structure. We plan to sequence and annotate the largest bread wheat chromosome, 3B (1 Gb i.e. 2.5x the rice genome) using a hybrid approach combining Roche454 Titanium sequencing of BACs and Whole Chromosome Shotgun (WCS) with Illumina/Solexa GAII PE reads. In a pilot project, we sequenced and annotated 13 large contigs (18 Mb) ...

  8. Collimated Outflow Formation via Binary Stars. 3-D Simulations of AGB Wind and Disk Wind Interactions

    OpenAIRE

    Garcia-Arredondo, F.; Frank, Adam

    2003-01-01

    We present three-dimensional hydrodynamic simulations of the interaction of a slow wind from an asymptotic giant branch(AGB) star and a jet blown by an orbiting companion. The jet or "Collimated Fast Wind" is assumed to originate from an accretion disk which forms via Bondi accretion of the AGB wind or Roche lobe overflow. We present two distinct regimes in the wind-jet interaction determined by the ratio of the AGB wind to jet momentum flux. Our results show that when the wind momentum flux ...

  9. Determination of renal clearances of amylase/creatinine with chromogenic and enzymatic methods.

    Science.gov (United States)

    Hohenwallner, W; Wimmer, E; Sommer, R

    1979-12-01

    Urinary amylase was estimated by chromogenic (amylochrome Roche) as well as enzymatic methods (SKI and Beckman: substrate starch and substrate maltotetraose respectively). Random and timed urines (24 hour collections) were analysed. Clearances of amylase gave different results dependent upon the amylase-test used and the glomerular filtration rate. Correlation between chromogenic and enzymatic methods (starch as substrate) was poor. The ratio of amylase and creatinine clearance was used to test different methods. Reference values for this ratio for the amylochrome method (N = 106) were 2.85 +/- 0.99% and for the Beckman-DS method (N = 60) 2.82 +/- 0.87%.

  10. Computing a Function of Correlated Sources

    CERN Document Server

    Sefidgaran, Milad

    2011-01-01

    A receiver wants to compute a function f of two correlated sources X and Y and side information Z. What is the minimum number of bits that needs to be communicated by each transmitter? In this paper, we derive inner and outer bounds to the rate region of this problem which coincide in the cases where f is partially invertible and where the sources are independent given the side information. From the former case we recover the Slepian-Wolf rate region and from the latter case we recover Orlitsky and Roche's single source result.

  11. Stability of Gas Clouds in Galactic Nuclei: An Extended Virial Theorem

    OpenAIRE

    Chen, X; Amaro-Seoane, P.; Cuadra, J.

    2015-01-01

    Cold gas entering the central $1$ to $10^2$ pc of a galaxy fragments and condenses into clouds. The stability of the clouds determines whether they will be turned into stars or can be delivered to the central supermassive black hole (SMBH) to turn on an active galactic nucleus (AGN). The conventional criteria to assess the stability of these clouds, such as the Jeans criterion and Roche (or tidal) limit, are insufficient here, because they assume the dominance of self-gravity in binding a clo...

  12. Ultra-stripped supernovae and double neutron star systems

    CERN Document Server

    Tauris, Thomas

    2015-01-01

    The evolution of close-orbit progenitor binaries of double neutron star (DNS) systems leads to supernova (SN) explosions of ultra-stripped stars. The amount of SN ejecta mass is very limited from such, more or less, naked metal cores with envelope masses of only 0.01-0.2 Msun. The combination of little SN ejecta mass and the associated possibility of small NS kicks is quite important for the characteristics of the resulting DNS systems left behind. Here, we discuss theoretical predictions for DNS systems, based on Case BB Roche-lobe overflow prior to ultra-stripped SNe, and briefly compare with observations.

  13. Analytical performance specifications: relating laboratory performance to quality required for intended clinical use.

    Science.gov (United States)

    Dalenberg, Daniel A; Schryver, Patricia G; Klee, George G

    2013-03-01

    This article proposes analytic performance goals for five quality indicators: precision, trueness, linearity, detection limits, and consistency across instruments and time. We defined our goals using methods linked to clinical practice data. Goals for desirable precision and trueness are based on biological variation. Linearity goals are related to total error recommendations. Detection limit goals are derived from 0.1 percentile of patient values. Goals for consistency are derived from the variability of distributions of patient test values. Data were collected and evaluated for each of these quality indicators for 46 chemistry tests measured on the Roche cobas 8000 analyzer.

  14. L'atténuation intrinsèque des ondes sismiques. Troisième partie : Mécanismes régissant l'atténuation. Intrinsic Attenuation of Seismic Waves. Part Three: Mechanisms Governing Attenuation

    Directory of Open Access Journals (Sweden)

    Bourbie T.

    2006-11-01

    Full Text Available Les mécanismes qui régissent l'atténuation sont très complexes et encore mal compris actuellement. Certains mécanismes ont pu être éliminés comme le phénomène de frottement grain à grain du fait des amplitudes de déformation usuelles en sismique. Il. semble que les mécanismes responsables soient différents dans le cas de roches sèches (aux conditions de laboratoire et dans le cas de roches plus ou moins saturées. Dans le cas de roches sèchesl'atténuation semble provenir à la fois d'un hystérésis microcapillaire et d'une rupture des liaisons hydrogènes entre les hydroxyles de la surface et les molécules d'eau. Dans le cas de roches saturées, les candidats les plus probables au phénomène d'atténuation sont les mécanismes de Biot et de squirt flow . Le mécanisme de Biot est la possibilité d'avoir des mouvements différentiels globaux (ou plutôt moyennés à l'échelle de l'échantillon entre la matière solide et le fluide. Le squirt flowconsiste en un phénomène de relaxation visqueuse dont l'excitateur est extérieur : le liquide à l'intérieur de la cavité poreuse jaillit sous l'effet d'une pression de pore locale. The mechanisms governing attenuation are highly complex and still poorly understood at present. Some mechanisms have been eliminated, such as the grain-to-grain friction, due to the usual deformation amplitudes in seismic exploration. The mechanisms responsible for attenuation seem to be different for dry rocks (under laboratory conditions and more or less saturated rocks. For dryrocks, attenuation seems to come from both a microcapillary hysteresis and a break of the hydrogen bonds between the surface hydroxyls and water molecules. For saturated rocks, the most probable causes of attenuation are Biot mechanisms and squirt flow. The Biot mechanism Is the possibility of having overall differential movements (or rather averaged ones on the scale of the sample between the solid matrix and the fluid

  15. DETECTION OF ACCRETION X-RAYS FROM QS Vir: CATACLYSMIC OR A LOT OF HOT AIR?

    Energy Technology Data Exchange (ETDEWEB)

    Matranga, Marco; Drake, Jeremy J.; Kashyap, Vinay [Harvard-Smithsonian Center for Astrophysics, 60 Garden Street, Cambridge, MA 02138 (United States); Steeghs, Danny, E-mail: mmatranga@cfa.harvard.edu [Department of Physics, University of Warwick, Coventry CV4 7AL (United Kingdom)

    2012-03-10

    An XMM-Newton observation of the nearby 'pre-cataclysmic' short-period (P{sub orb} = 3.62 hr) binary QS Vir (EC 13471-1258) revealed regular narrow X-ray eclipses when the white dwarf passed behind its M2-4 dwarf companion. The X-ray emission provides a clear signature of mass transfer and accretion onto the white dwarf. The low-resolution XMM-Newton EPIC spectra are consistent with a cooling flow model and indicate an accretion rate of M-dot = 1.7 Multiplication-Sign 10{sup -13} M{sub sun} yr{sup -1}. At 48 pc distant, QS Vir is then the second nearest accreting cataclysmic variable known, with one of the lowest accretion rates found to date for a non-magnetic system. To feed this accretion through a wind would require a wind mass-loss rate of M-dot {approx}2 Multiplication-Sign 10{sup -12} M{sub sun} yr{sup -1} if the accretion efficiency is of the order of 10%. Consideration of likely mass-loss rates for M dwarfs suggests this is improbably high and pure wind accretion unlikely. A lack of accretion disk signatures also presents some difficulties for direct Roche lobe overflow. We speculate that QS Vir is on the verge of Roche lobe overflow, and that the observed mass transfer could be supplemented by upward chromospheric flows on the M dwarf, analogous to spicules and mottles on the Sun, that escape the Roche surface to be subsequently swept up into the white dwarf Roche lobe. If so, QS Vir would be in a rare evolutionary phase lasting only a million years. The X-ray luminosity of the M dwarf estimated during primary eclipse is L{sub X} = 3 Multiplication-Sign 10{sup 28} erg s{sup -1}, which is consistent with that of rapidly rotating 'saturated' K and M dwarfs.

  16. The genome and transcriptome of perennial ryegrass mitochondria

    DEFF Research Database (Denmark)

    Islam, Md. Shofiqul; Studer, Bruno; Byrne, Stephen;

    2013-01-01

    and annotation of the complete mitochondrial genome from perennial ryegrass. Results: Intact mitochondria from perennial ryegrass leaves were isolated and used for mtDNA extraction. The mitochondrial genome was sequenced to a 167-fold coverage using the Roche 454 GS-FLX Titanium platform, and assembled...... of the mitochondrial genome from perennial ryegrass presented here constitutes an important tool for future attempts to compare mitochondrial genomes within and between grass species. Our results also demonstrate that mitochondria of perennial ryegrass contain genes crucial for energy production that are well...

  17. D-Branes, RR-Fields and Duality on Noncommutative Manifolds

    CERN Document Server

    Brodzki, J; Rosenberg, J; Szabó, R J; Brodzki, Jacek; Mathai, Varghese; Rosenberg, Jonathan; Szabo, Richard J.

    2006-01-01

    We develop some of the ingredients needed for string theory on noncommutative spacetimes, proposing an axiomatic formulation of T-duality as well as establishing a very general formula for D-brane charges. This formula is closely related to a noncommutative Grothendieck-Riemann-Roch theorem that is proved here. Our approach relies on a very general form of Poincare duality, which is studied here in detail. Among the technical tools employed are calculations with iterated products in bivariant K-theory and cyclic theory, which are simplified using a novel diagram calculus reminiscent of Feynman diagrams.

  18. The contribution of air-fluidization to the mobility of rapid flowslides involving fine particles

    Science.gov (United States)

    Stilmant, Frédéric; Dewals, Benjamin; Archambeau, Pierre; Erpicum, Sébastien; Pirotton, Michel

    2016-04-01

    Air-fluidization can be the origin of the long runout of gravitational flows involving fine particles such as ash. An excessive air pore pressure dramatically reduces the friction angle of the material as long as this pressure has not been dissipated, which occurs during the flow. This phenomenon can be modelled thanks to the 2D depth-averaged equations of mass and momentum conservation and an additional transport equation for basal pore pressure evolution (Iverson and Denlinger, 2001). In this contribution, we discuss the application of this model in relation to recent experimental results on air-fluidized flows by Roche et al. (2008) and Roche (2012). The experimental results were used to set a priori the value of the diffusion coefficient in the model, taking into account the difference of scale between the experiments and real-world applications. We also compare the model predictions against detailed observations of a well-documented historical event, the collapse of a fly-ash heap in Belgium (Stilmant et al., 2015). In particular, we analyse the influence of the different components of the model on the results (pore pressure dissipation vs. pore pressure generation). The diffusion coefficient which characterizes the dissipation of air pore pressure is found sufficiently low for maintaining a fluidized flow over hundreds of meters. The study concludes that an air-fluidization theory is consistent with the field observations. These findings are particularly interesting as they seem not in line with the mainstream acceptation in landslide modelling that air generally plays a secondary role (e.g., Legros, 2002). References Iverson, R.M., Denlinger, R.P., 2001. Flow of variably fluidized granular masses across three-dimensional terrain - 1. Coulomb mixture theory. J. Geophys. Res. 106, 537 552. Legros, F., 2002. The mobility of long-runout landslides. Eng. Geol. 63, 301-331. Roche, O., 2012. Depositional processes and gas pore pressure in pyroclastic flows: an

  19. WTS-2 b: Too close for comfort?

    OpenAIRE

    Kovács G.; Sipőcz B.; Pinfield D.J.; Hodgkin S.T.; Mustill A.; Ivanyuk O.; Koppenhoefer J.; Cruz P; Cappetta M.; Birkby J.L.; Saglia R.; Pavlenko Y.

    2013-01-01

    We report the discovery of WTS-2 b, a typical hot Jupiter in an unusually close 1.02-day orbit to a K-dwarf star. This is the second planet to be discovered in the infrared light curves of the WFCAM Transit Survey (WTS) and is only one-and-a-half times the separation from its host star at which is would be destroyed by Roche lobe overflow. The predicted remaining lifetime of the planet is just 38 Myrs, assuming a tidal dissipation quality factor of Q'* = 106. The magnitude of Q'* is largely u...

  20. Evolution des escarpements calcaires instables. Quantification des mouvements actuels par G.P.S. et inclinométrie dans les Alpes du nord (France)

    OpenAIRE

    Marconnet, Jean François

    1999-01-01

    Les escarpements des massifs calcaires alpins sont particulièrement sensibles aux instabilités à cause de leur structure stratifiée alternant des couches de compétences différentes et des nombreux facteurs favorisant leur érosion, tels que la présence d'eau ou de glace dans les fissures de la roche. L'instabilité des escarpements calcaires se manifeste par des éboulements ou des mouvements progressifs de masses rocheuses. Ces derniers mouvements ont été quantifiés sur deux escarpements à lith...

  1. Specificity of the Linear Array HPV Genotyping Test for detecting human papillomavirus genotype 52 (HPV-52):

    OpenAIRE

    Kocjan, Boštjan; Poljak, Mario; Oštrbenk, Anja

    2014-01-01

    Introduction: HPV-52 is one of the most frequent human papillomavirus (HPV) genotypes causing significant cervical pathology. The most widely used HPV genotyping assay, the Roche Linear Array HPV Genotyping Test (Linear Array), is unable to identify HPV- 52 status in samples containing HPV-33, HPV-35, and/or HPV-58. Methods: Linear Array HPV-52 analytical specificity was established by testing 100 specimens reactive with the Linear Array HPV- 33/35/52/58 cross-reactive probe, but not with the...

  2. Sergeac

    OpenAIRE

    White, Randall; Mensan, Romain; Sisk, Matthew; Clark, Amy; toute l'équipe, Castenet

    2013-01-01

    Identifiant de l'opération archéologique : 0225467 Date de l'opération : 2005 (FP) Bilan des fouilles programmées (Aurignacien ancien)      • L’abri Castanet L’abri effondré dénommé  Castanet  orienté plein ouest, est situé sur la commune de Sergeac, sur la rive droite du vallon de Castel Merle ou vallon des Roches. Après un premier sondage de Marcel Castanet, ce site fut fouillé ...

  3. NOTE: Explaining why the Uranian satellites have equatorial prograde orbits despite the large planetary obliquity

    OpenAIRE

    Morbidelli, Alessandro; Tsiganis, Kleomenis; Batygin, Konstantin; Crida, Aurelien; Gomes, Rodney

    2012-01-01

    We show that the existence of prograde equatorial satellites is consistent with a collisional tilting scenario for Uranus. In fact, if the planet was surrounded by a proto-satellite disk at the time of the tilting and a massive ring of material was temporarily placed inside the Roche radius of the planet by the collision, the proto-satellite disk would have started to precess incoherently around the equator of the planet, up to a distance greater than that of Oberon. Collisional damping would...

  4. [Cutaneous melanoma - "black death" of modern times? Traces in contemporary literature].

    Science.gov (United States)

    Bahmer, F A; Bahmer, J A

    2013-11-01

    Cutaneous melanoma, sometimes labeled as "black skin cancer", is increasing in frequency and becoming a more common literary motive. In US literature, Sylvia Plath and Charles Bukowski depicted melanoma more than 50 years ago, later Stephen King and Thomas C. Boyle. In German literature, Charlotte Roche shortly mentioned this tumor. Jörg Pönnighaus, both poet and dermatologist, intensively deals in his poems with the effects melanoma has on patients and doctors alike. Melanoma definitely is not the "Black Death" of modern times. However, the perception of this tumor as extremely malignant and as life-threatening makes melanoma a metaphor of the deadly danger of cancer.

  5. SNP Discovery In Marine Fish Species By 454 Sequencing

    DEFF Research Database (Denmark)

    Panitz, Frank; Nielsen, Rasmus Ory; van Houdt, Jeroen K J;

    2011-01-01

    Based on the 454 Next-Generation-Sequencing technology (Roche) a high throughput screening method was devised in order to generate novel genetic markers (SNPs). SNP discovery was performed for three target species of marine fish: hake (Merluccius merluccius), herring (Clupea harengus) and sole...... (Solea solea). For each species, a total of 7-8 individuals from 4-5 populations, that in most cases spanned major parts of the species’ distributions, were individually tagged and subsequently pooled for cDNA library construction. After 454 sequencing reads were de-multiplexed, cleaned, repeat...

  6. Recharacterization of ancient DNA miscoding lesions: insights in the era of sequencing-by-synthesis

    DEFF Research Database (Denmark)

    Gilbert, M Thomas P; Binladen, Jonas; Miller, Webb;

    2007-01-01

    Although ancient DNA (aDNA) miscoding lesions have been studied since the earliest days of the field, their nature remains a source of debate. A variety of conflicting hypotheses exist about which miscoding lesions constitute true aDNA damage as opposed to PCR polymerase amplification error...... strand of origin of observed damage events. With the advent of emulsion-based clonal amplification (emPCR) and the sequencing-by-synthesis technology this has changed. In this paper we demonstrate how data produced on the Roche GS20 genome sequencer can determine miscoding lesion strands of origin...

  7. Molecular diet analysis of two African free-tailed bats (Molossidae) using high throughput sequencing

    DEFF Research Database (Denmark)

    Bohmann, Kristine; Monadjem, Ara; Noer, Christina Lehmkuhl;

    2011-01-01

    Given the diversity of prey consumed by insectivorous bats, it is difficult to discern the composition of their diet using morphological or conventional PCR-based analyses of their faeces. We demonstrate the use of a powerful alternate tool, the use of the Roche FLX sequencing platform to deep......-sequence uniquely 5′ tagged insect-generic barcode cytochrome c oxidase I (COI) fragments, that were PCR amplified from faecal pellets of two free-tailed bat species Chaerephon pumilus and Mops condylurus (family: Molossidae). Although the analyses were challenged by the paucity of southern African insect COI...

  8. Genome sequencing and analysis of a granulovirus isolated from the Asiatic rice leafroller, Cnaphalocrocis medinalis.

    Science.gov (United States)

    Zhang, Shan; Zhu, Zheng; Sun, Shifeng; Chen, Qijin; Deng, Fei; Yang, Kai

    2015-12-01

    The complete genome of Cnaphalocrocis medinalis granulovirus (CnmeGV) from a serious migratory rice pest, Cnaphalocrocis medinalis (Lepidoptera: Pyralidae), was sequenced using the Roche 454 Genome Sequencer FLX system (GS FLX) with shotgun strategy and assembled by Roche GS De Novo assembler software. Its circular double-stranded genome is 111,246 bp in size with a high A+T content of 64.8% and codes for 118 putative open reading frames (ORFs). It contains 37 conserved baculovirus core ORFs, 13 unique ORFs, 26 ORFs that were found in all Lepidoptera baculoviruses and 42 common ORFs. The analysis of nucleotide sequence repeats revealed that the CnmeGV genome differs from the rest of sequenced GVs by a 23 kb and a 17kb gene block inversions, and does not contain any typical homologous region (hr) except for a region of non-hr-like sequence. Chitinase and cathepsin genes, which are reported to have major roles in the liquefaction of the hosts, were not found in the CnmeGV genome, which explains why CnmeGV infected insects do not show the phenotype of typical liquefaction. Phylogenetic analysis, based on the 37 core baculovirus genes, indicates that CnmeGV is closely related to Adoxophyes orana granulovirus. The genome analysis would contribute to the functional research of CnmeGV, and would benefit to the utilization of CnmeGV as pest control reagent for rice production.

  9. The first pre-supersoft X-ray binary

    CERN Document Server

    Parsons, S G; Gansicke, B T; Rebassa-Mansergas, A; Brahm, R; Zorotovic, M; Toloza, O; Pala, A F; Tappert, C; Bayo, A; Jordan, A

    2015-01-01

    We report the discovery of an extremely close white dwarf plus F dwarf main-sequence star in a 12 hour binary identified by combining data from the RAdial Velocity Experiment (RAVE) survey and the Galaxy Evolution Explorer (GALEX) survey. A combination of spectral energy distribution fitting and optical and Hubble Space Telescope ultraviolet spectroscopy allowed us to place fairly precise constraints on the physical parameters of the binary. The system, TYC 6760-497-1, consists of a hot Teff~21,500K, M~0.65Ms white dwarf and an F8 star (M~1.23Ms, R~1.35Rs) seen at a low inclination (i~35 deg). The system is likely the descendent of a binary that contained the F star and a ~2Ms A-type star that filled its Roche-lobe on the second asymptotic giant branch, initiating a common envelope phase. The F star is extremely close to Roche-lobe filling and there is likely to be a short phase of thermal timescale mass-transfer onto the white dwarf. During this phase it will grow in mass by up to 20 per cent, until the mass...

  10. Facts and fictions about polymorphism.

    Science.gov (United States)

    Cruz-Cabeza, Aurora J; Reutzel-Edens, Susan M; Bernstein, Joel

    2015-12-01

    We present new facts about polymorphism based on (i) crystallographic data from the Cambridge Structural Database (CSD, a database built over 50 years of community effort), (ii) 229 solid form screens conducted at Hoffmann-La Roche and Eli Lilly and Company over the course of 8+ and 15+ years respectively and (iii) a dataset of 446 polymorphic crystals with energies and properties computed with modern DFT-d methods. We found that molecular flexibility or size has no correlation with the ability of a compound to be polymorphic. Chiral molecules, however, were found to be less prone to polymorphism than their achiral counterparts and compounds able to hydrogen bond exhibit only a slightly higher propensity to polymorphism than those which do not. Whilst the energy difference between polymorphs is usually less than 1 kcal mol(-1), conformational polymorphs are capable of differing by larger values (up to 2.5 kcal mol(-1) in our dataset). As overall statistics, we found that one in three compounds in the CSD are polymorphic whilst at least one in two compounds from the Roche and Lilly set display polymorphism with a higher estimate of up to three in four when compounds are screened intensively. Whilst the statistics provide some guidance of expectations, each compound constitutes a new challenge and prediction and realization of targeted polymorphism still remains a holy grail of materials sciences.

  11. Formation of diverse ring-satellite systems around Centaurs through tidal disruption at close encounters with giant planet

    CERN Document Server

    Hyodo, Ryuki; Genda, Hidenori; Ohtsuki, Keiji

    2016-01-01

    Centaurs are minor planets orbiting between Jupiter and Neptune that have or had crossing orbits with one or more giant planets. Recent observations and reinterpretation of previous observations have revealed the existence of ring systems around 10199 Chariklo and 2060 Chiron. However, the origin of the ring systems around such a minor planet is still an open question. Here, we propose that the tidal disruption of a differentiated object that experiences a close encounter with a giant planet could naturally form diverse ring-satellite systems around the Centaurs. During the close encounter, the icy mantle of the passing object is preferentially ripped off by the planet's tidal force and the debris is distributed mostly within the Roche limit of the largest remnant body. Assuming the existence of $20-50$wt% silicate core below the icy mantle, a disk of particles is formed when the objects pass within $0.4-0.8$ of the planet's Roche limit with the relative velocity at infinity $3-6$km s$^{-1}$ and 8h initial sp...

  12. Elements of Poro-Elasticity for Reservoir Engineering Éléments de poro-élasticité pour l'ingénierie de réservoirs

    Directory of Open Access Journals (Sweden)

    Bouteca M.

    2006-11-01

    -élasticité utiles à l'ingénieur de réservoir. La poro-élasticité permet de calculer les déformations du solide liées au mouvement du fluide. C'est donc le cadre théorique qui permet de définir la compressibilité de la roche. Nous montrons ainsi, dans un premier temps, comment les variables mécaniques précédemment introduites sont reliées à la compressibilité de la roche mesurée expérimentalement. Deux hypothèses sont retenues : a mesure en condition isotrope (définie par une pression de confinement et une pression de pore, b mesure en condition oedométrique (définie par un chargement vertical, un déplacement radial nul et une pression de pore. Les principaux résultats sont illustrés dans le tableau 6. On montre en outre qu'en condition isotrope, la compressibilité du volume poreux peut être mesurée indifféremment à pression de pore variable et pression de confinement constante ou à pression de pore constante et pression de confinement variable (tableau 5. Dans un deuxième temps nous examinons sous quelles conditions l'équation de diffusivité définie dans la première partie peut être découplée de l'équation d'équilibre du solide. On montre que la contribution de la déformation de la roche à l'écoulement peut être exprimée à l'aide d'un coefficient de compressibilité à condition que l'on fasse des hypothèses sur le mode de déformation local de la roche. Rappelons que, par opposition à des conditions aux limites qui sont vraies sur le contour du domaine, les conditions locales sont vraies en tout point et constituent une simplification du problème réel. En conclusion, on souligne l'importance du choix des conditions locales retenues pour la mesure de la compressibilité de la roche. La compressibilité de la roche étant définie, l'équation de diffusivité prend sa forme classique : la contribution de la roche est introduite en définissant une compressibilité totale somme de la compressibilité du fluide et de celle de la roche.

  13. Simultaneous detection of human mitochondrial DNA and nuclear-inserted mitochondrial-origin sequences (NumtS) using forensic mtDNA amplification strategies and pyrosequencing technology.

    Science.gov (United States)

    Bintz, Brittania J; Dixon, Groves B; Wilson, Mark R

    2014-07-01

    Next-generation sequencing technologies enable the identification of minor mitochondrial DNA variants with higher sensitivity than Sanger methods, allowing for enhanced identification of minor variants. In this study, mixtures of human mtDNA control region amplicons were subjected to pyrosequencing to determine the detection threshold of the Roche GS Junior(®) instrument (Roche Applied Science, Indianapolis, IN). In addition to expected variants, a set of reproducible variants was consistently found in reads from one particular amplicon. A BLASTn search of the variant sequence revealed identity to a segment of a 611-bp nuclear insertion of the mitochondrial control region (NumtS) spanning the primer-binding sites of this amplicon (Nature 1995;378:489). Primers (Hum Genet 2012;131:757; Hum Biol 1996;68:847) flanking the insertion were used to confirm the presence or absence of the NumtS in buccal DNA extracts from twenty donors. These results further our understanding of human mtDNA variation and are expected to have a positive impact on the interpretation of mtDNA profiles using deep-sequencing methods in casework.

  14. Formation of the Double Neutron Star System PSR J1930$-$1852

    CERN Document Server

    Shao, Yong

    2015-01-01

    The spin period (185 ms) and period derivative ($1.8\\times10^{-17}\\,\\rm s\\,s^{-1}$) of the double neutron star (DNS) system PSR J1930$-$1852 recently discovered indicate that the pulsar was mildly recycled through the process of Roche-lobe overflow. This system has the longest orbital period (45 days) of the known DNS systems, and can be formed from a helium star-NS binary if the initial mass of the helium star was $ \\lesssim 4.0M_{\\odot} $; otherwise the helium star would never fill its Roche-lobe \\citep{t15}. At the moment of the supernova explosion, the mass of the helium star was $ \\lesssim3.0M_{\\odot} $. We find that the probability distribution of the velocity kick imparted to the new-born neutron star has a maximum at about $30 \\,\\rm km\\,s^{-1}$ (and a tail up to $ 260 \\,\\rm km\\,s^{-1}$), indicating that this NS most probably received a low kick velocity at birth.

  15. STABILITY OF GAS CLOUDS IN GALACTIC NUCLEI: AN EXTENDED VIRIAL THEOREM

    Energy Technology Data Exchange (ETDEWEB)

    Chen, Xian; Cuadra, Jorge [Instituto de Astrofísica, Facultad de Física, Pontificia Universidad Católica de Chile, 782-0436 Santiago (Chile); Amaro-Seoane, Pau, E-mail: xchen@astro.puc.cl, E-mail: jcuadra@astro.puc.cl, E-mail: Pau.Amaro-Seoane@aei.mpg.de [Max Planck Institut für Gravitationsphysik (Albert-Einstein-Institut), D-14476 Potsdam (Germany)

    2016-03-10

    Cold gas entering the central 1–10{sup 2} pc of a galaxy fragments and condenses into clouds. The stability of the clouds determines whether they will be turned into stars or can be delivered to the central supermassive black hole (SMBH) to turn on an active galactic nucleus (AGN). The conventional criteria to assess the stability of these clouds, such as the Jeans criterion and Roche (or tidal) limit, are insufficient here, because they assume the dominance of self-gravity in binding a cloud, and neglect external agents, such as pressure and tidal forces, which are common in galactic nuclei. We formulate a new scheme for judging this stability. We first revisit the conventional Virial theorem, taking into account an external pressure, to identify the correct range of masses that lead to stable clouds. We then extend the theorem to further include an external tidal field, which is equally crucial for the stability in the region of our interest—in dense star clusters, around SMBHs. We apply our extended Virial theorem to find new solutions to controversial problems, namely, the stability of the gas clumps in AGN tori, the circum-nuclear disk in the Galactic Center, and the central molecular zone of the Milky Way. The masses we derive for these structures are orders of magnitude smaller than the commonly used Virial masses (equivalent to the Jeans mass). Moreover, we prove that these clumps are stable, contrary to what one would naively deduce from the Roche (tidal) limit.

  16. Close Binary System GO Cyg

    CERN Document Server

    Ulas, B; Keskin, V; Kose, O; Yakut, K

    2011-01-01

    In this study, we present long term photometric variations of the close binary system \\astrobj{GO Cyg}. Modelling of the system shows that the primary is filling Roche lobe and the secondary of the system is almost filling its Roche lobe. The physical parameters of the system are $M_1 = 3.0\\pm0.2 M_{\\odot}$, $M_2 = 1.3 \\pm 0.1 M_{\\odot}$, $R_1 = 2.50\\pm 0.12 R_{\\odot}$, $R_2 = 1.75 \\pm 0.09 R_{\\odot}$, $L_1 = 64\\pm 9 L_{\\odot}$, $L_2 = 4.9 \\pm 0.7 L_{\\odot}$, and $a = 5.5 \\pm 0.3 R_{\\odot}$. Our results show that \\astrobj{GO Cyg} is the most massive system near contact binary (NCB). Analysis of times of the minima shows a sinusoidal variation with a period of $92.3\\pm0.5$ years due to a third body whose mass is less than 2.3$M_{\\odot}$. Finally a period variation rate of $-1.4\\times10^{-9}$ d/yr has been determined using all available light curves.

  17. Understanding the evolution of close binary systems with radio pulsars

    CERN Document Server

    Benvenuto, O G; Horvath, J E

    2014-01-01

    We calculate the evolution of close binary systems (CBSs) formed by a neutron star (behaving as a radio pulsar) and a normal donor star, evolving either to helium white dwarf (HeWD) or ultra short orbital period systems. We consider X-ray irradiation feedback and evaporation due to radio pulsar irradiation. We show that irradiation feedback leads to cyclic mass transfer episodes, allowing CBSs to be observed in-between as binary radio pulsars under conditions in which standard, non-irradiated models predict the occurrence of a low mass X-ray binary. This behavior accounts for the existence of a family of eclipsing binary systems known as redbacks. We predict that redback companions should almost fill their Roche lobe, as observed in PSR J1723-2837. This state is also possible for systems evolving with larger orbital periods. Therefore, binary radio pulsars with companion star masses usually interpreted as larger than expected to produce HeWDs may also result in such {\\it quasi - Roche Lobe Overflow} states, r...

  18. A Neutron Star-White Dwarf Binary Model for Repeating Fast Radio Burst 121102

    CERN Document Server

    Gu, Wei-Min; Liu, Tong; Ma, Renyi; Wang, Junfeng

    2016-01-01

    We propose a compact binary model for the fast radio burst (FRB) repeaters, where the system consists of a magnetic white dwarf (WD) and a neutron star (NS) with strong bipolar magnetic fields. When the WD fills its Roche lobe, mass transfer will occur from the WD to the NS through the inner Lagrange point. The accreted magnetized materials may trigger magnetic reconnection when they approach the NS surface, and therefore the electrons can be accelerated to an ultra-relativistic speed. In this scenario, the curvature radiation of the electrons moving along the NS magnetic field lines can account for the characteristic frequency and the timescale of an FRB. Owing to the conservation of angular momentum, the WD may be kicked away after a burst, and the next burst may appear when the system becomes semi-detached again through the gravitational radiation. By comparing our analyses with the observations, we show that such an intermittent Roche lobe overflow mechanism can be responsible for the observed repeating b...

  19. Period studies and photometric models for two EB-type binaries EU Hya and AW Vul

    Science.gov (United States)

    Yang, Yuan-Gui; Li, Qun; Li, Hua-Li; Dai, Hai-Feng

    2016-02-01

    New photometry for two Algol-type binaries, EU Hya (P = 0.7782 d) and AW Vul (P = 0.8065 d), was carried out using the 60-cm telescope at Xinglong station of National Astronomical Observatories, Chinese Academy of Sciences. With the updated Wilson-Devinney code, photometric elements were derived from the multi-color light curves. The modeled results indicate that the two systems are near-contact binaries, whose secondary components fill their Roche lobes. The fill-out factors of the primaries are fp = 81.2 (±0.2)% for EU Hya and fp = 82.4 (±0.3)% for AW Vul. Period analysis implies that there exists a downward parabola with a light-time orbit from the (O - C) curve. This kind of periodic oscillation may be attributed to the light-time orbit effect of a third companion. The long-term period decrease may be caused by mass and angular momentum loss. When the orbital period decreases, the fill-out factor of fp will increase. Our results indicate that the primaries will also eventually fill their Roche lobes. EU Hya and AW Vul may possibly evolve from semi-detached binaries into contact ones.

  20. The coupled effect of tides and stellar winds on the evolution of compact binaries

    CERN Document Server

    Repetto, Serena

    2014-01-01

    We follow the evolution of compact binaries under the coupled effect of tides and stellar winds until the onset of Roche-lobe overflow. These binaries contain a compact object (either a black-hole, a neutron-star, or a planet) and a stellar component. We integrate the full set of tidal equations, which are based on Hut's model for tidal evolution, and we couple them with the angular momentum loss in a stellar wind. Our aim is twofold. Firstly, we wish to highlight some interesting evolutionary outcomes of the coupling. When tides are coupled with a non-massive stellar wind, one interesting outcome is that in certain types of binaries, the stellar spin tends to reach a quasi-equilibrium state, where the effect of tides and wind are counteracting each other. When tides are coupled with a massive wind, we parametrize the evolution in terms of the decoupling radius, at which the wind decouples from the star. Even for small decoupling radii this \\emph{wind braking} can drive systems on the main sequence to Roche-l...

  1. A comprehensive photometric study of the eclipsing binary EP Aurigae

    Science.gov (United States)

    Li, H.-L.; Wei, J.-Y.; Yang, Y.-G.; Li, K.; Zhang, X.-B.

    2015-02-01

    We present new observations for the eclipsing binary EP Aurigae, which were performed by using three small telescopes in China from 2003 December to 2014 January. With the updated 2003 version of the Wilson-Devinney code, the photometric elements were deduced from three sets of light curves. Based on all available eclipsing times, the orbital period changes were investigated. It is discovered that the (O-C) curve may show an existence of light-time effect due to an unseen third body, which was weakly identified by the photometric solution. The modulated period and amplitude of the cyclic variation are P3=71.2(±8.0) yr and A=0.0101(±0.0008) day, respectively. In the co-planar orbit with the binary system, the mass of the third body is M3=0.18(±0.02) M⊙. The photometric results imply that EP Aur is an Algol-type binary with a mass ratio of q=0.831(±0.004). Its primary component almost fills its Roche lobe. Therefore, EP Aur may consist of a normal main-sequence star and a cool Roche-lobe filling subgiant, which may be undergoing rapid mass transfer.

  2. Dissection of the octoploid strawberry genome by deep sequencing of the genomes of Fragaria species.

    Science.gov (United States)

    Hirakawa, Hideki; Shirasawa, Kenta; Kosugi, Shunichi; Tashiro, Kosuke; Nakayama, Shinobu; Yamada, Manabu; Kohara, Mistuyo; Watanabe, Akiko; Kishida, Yoshie; Fujishiro, Tsunakazu; Tsuruoka, Hisano; Minami, Chiharu; Sasamoto, Shigemi; Kato, Midori; Nanri, Keiko; Komaki, Akiko; Yanagi, Tomohiro; Guoxin, Qin; Maeda, Fumi; Ishikawa, Masami; Kuhara, Satoru; Sato, Shusei; Tabata, Satoshi; Isobe, Sachiko N

    2014-01-01

    Cultivated strawberry (Fragaria x ananassa) is octoploid and shows allogamous behaviour. The present study aims at dissecting this octoploid genome through comparison with its wild relatives, F. iinumae, F. nipponica, F. nubicola, and F. orientalis by de novo whole-genome sequencing on an Illumina and Roche 454 platforms. The total length of the assembled Illumina genome sequences obtained was 698 Mb for F. x ananassa, and ∼200 Mb each for the four wild species. Subsequently, a virtual reference genome termed FANhybrid_r1.2 was constructed by integrating the sequences of the four homoeologous subgenomes of F. x ananassa, from which heterozygous regions in the Roche 454 and Illumina genome sequences were eliminated. The total length of FANhybrid_r1.2 thus created was 173.2 Mb with the N50 length of 5137 bp. The Illumina-assembled genome sequences of F. x ananassa and the four wild species were then mapped onto the reference genome, along with the previously published F. vesca genome sequence to establish the subgenomic structure of F. x ananassa. The strategy adopted in this study has turned out to be successful in dissecting the genome of octoploid F. x ananassa and appears promising when applied to the analysis of other polyploid plant species. PMID:24282021

  3. Prevalence of use study for amphetamine (AMP), methamphetamine (MAMP), 3,4-methylenedioxy-amphetamine (MDA), 3,4-methylenedioxy-methamphetamine (MDMA), and 3,4-methylenedioxy-ethylamphetamine (MDEA) in military entrance processing stations (MEPS) specimens.

    Science.gov (United States)

    Klette, Kevin L; Kettle, Aaron R; Jamerson, Matthew H

    2006-06-01

    The Roche Abuscreen Onlinetrade mark Amphetamine immunoassay (IA), modified to include sodium periodate, and the Microgenics DRI Ecstasy IA were used to determine the prevalence of amphetamine (AMP), methamphetamine (MAMP), 3,4-methylenedioxyamphetamine (MDA), 3,4-methylenedioxymethamphetamine (MDMA), and 3,4-methylenedioxyethylamphetamine (MDEA) in urine specimens from applicants seeking to join the United States Armed Forces. Over a 4-month period, a total of 85,658 specimens were IA screened using the Department of Defense 500 ng/mL administrative cutoff level for AMP and MDMA. All presumptively positive specimens were confirmed using a solid-phase extraction procedure coupled with simultaneous analysis of AMP, MAMP, MDA, MDMA, and MDEA by fast gas chromatography-mass spectrometry using the same cutoff levels as the IA. The Roche Online Amphetamine IA identified 216 specimens as presumptively positive; of these, 70 specimens confirmed positive for AMP and 87 specimens confirmed positive for AMP and/or MAMP, resulting in a confirmation rate of 73%. The Microgenics DRI Ecstasy IA identified eight specimens as presumptively positive; of these, five specimens confirmed positive for MDMA and/or MDA, resulting in a confirmation rate of 63%. The total use prevalence for AMP, MAMP, MDA, MDMA, and/or MDEA in military entrance processing stations specimens over the testing period was determined to be 0.19%.

  4. Stability of Gas Clouds in Galactic Nuclei: An Extended Virial Theorem

    Science.gov (United States)

    Chen, Xian; Amaro-Seoane, Pau; Cuadra, Jorge

    2016-03-01

    Cold gas entering the central 1-102 pc of a galaxy fragments and condenses into clouds. The stability of the clouds determines whether they will be turned into stars or can be delivered to the central supermassive black hole (SMBH) to turn on an active galactic nucleus (AGN). The conventional criteria to assess the stability of these clouds, such as the Jeans criterion and Roche (or tidal) limit, are insufficient here, because they assume the dominance of self-gravity in binding a cloud, and neglect external agents, such as pressure and tidal forces, which are common in galactic nuclei. We formulate a new scheme for judging this stability. We first revisit the conventional Virial theorem, taking into account an external pressure, to identify the correct range of masses that lead to stable clouds. We then extend the theorem to further include an external tidal field, which is equally crucial for the stability in the region of our interest—in dense star clusters, around SMBHs. We apply our extended Virial theorem to find new solutions to controversial problems, namely, the stability of the gas clumps in AGN tori, the circum-nuclear disk in the Galactic Center, and the central molecular zone of the Milky Way. The masses we derive for these structures are orders of magnitude smaller than the commonly used Virial masses (equivalent to the Jeans mass). Moreover, we prove that these clumps are stable, contrary to what one would naively deduce from the Roche (tidal) limit.

  5. Comparison and harmonization assessment of mutual among four different immunoassay systems in CA19-9 test%CA19-9测定在4个免疫检测系统的差异及互认能力的评估

    Institute of Scientific and Technical Information of China (English)

    宋超; 张曙云; 周俊; 单志明; 周永列

    2013-01-01

    目的 研究4个免疫检测系统对CA19-9检测的差异和一致性,探讨实现CA19-9检测结果互认的基础.方法 检测系统比对研究.汇集整理CA19-9室间质量评价活动批号的数据(卫生部200811 ~ 201215,浙江省080309 ~ 120211);搜集120份不同CA19-9浓度的新鲜血样本;搜集2010年9月至2012年3月不同检测系统非肿瘤体检人群实测CA19-9数据.选择4个稳定的检测系统Abbott Architect i2000、Roche E170、Beckman Dxi800和Siemens CentaurXP作为研究对象,统计分析在卫生部和浙江省室间质评(冻干粉和液体血清)中的组间差异;参照CLSI EP9-A2进行120份新鲜血样本的比对分析;计算各检测系统实测CA19-9的95%置信区间并分析.采用散点图、加权回归方程进行统计比较.结果 室间质评与新鲜血比对出现颠倒的结果.室间质评均表现出检测系统组间较大的偏倚,斜率bw在1.340到4.683之间,相关系数r较好;新鲜血比对中r不理想,但斜率bw接近于1.新鲜血比对中在推荐浓度27 U/ml时,检测系统两两间偏移分别为Abbott-Roche(-6.41%),Beckman-Roche(-5.07%),Siemens-Roche(13.15%),Beckman-Abbott(2.46%),Siemens-Abbott(22.52%),Siemens-Beckman(39.66%).部分检测系统间实测95%置信区间差异有统计学意义.结论 在研究检测系统差异时,在较低浓度CA19-9的结果在4个检测系统间具有互认的基础,但在高浓度时,检测系统间的差异明显增大,风险较高,尚无法实现互认.%Objective To investigate the differences and harmonization of immunoassay systems in detecting CA19-9 and to assess the possibility of mutual recognition in different laboratories.Methods Data were collected and analyzed from External Quality Assessments (EQA) of NCCL(Lots:200811-201215) and ZJCCL(Lots:080309-120211).120 fresh serum with different concentrations of CA19-9 were collected.The CA19-9 results of healthy people were also collected from September 2010 to March

  6. Improved Efficiency and Reliability of NGS Amplicon Sequencing Data Analysis for Genetic Diagnostic Procedures Using AGSA Software

    Directory of Open Access Journals (Sweden)

    Axel Poulet

    2016-01-01

    Full Text Available Screening for BRCA mutations in women with familial risk of breast or ovarian cancer is an ideal situation for high-throughput sequencing, providing large amounts of low cost data. However, 454, Roche, and Ion Torrent, Thermo Fisher, technologies produce homopolymer-associated indel errors, complicating their use in routine diagnostics. We developed software, named AGSA, which helps to detect false positive mutations in homopolymeric sequences. Seventy-two familial breast cancer cases were analysed in parallel by amplicon 454 pyrosequencing and Sanger dideoxy sequencing for genetic variations of the BRCA genes. All 565 variants detected by dideoxy sequencing were also detected by pyrosequencing. Furthermore, pyrosequencing detected 42 variants that were missed with Sanger technique. Six amplicons contained homopolymer tracts in the coding sequence that were systematically misread by the software supplied by Roche. Read data plotted as histograms by AGSA software aided the analysis considerably and allowed validation of the majority of homopolymers. As an optimisation, additional 250 patients were analysed using microfluidic amplification of regions of interest (Access Array Fluidigm of the BRCA genes, followed by 454 sequencing and AGSA analysis. AGSA complements a complete line of high-throughput diagnostic sequence analysis, reducing time and costs while increasing reliability, notably for homopolymer tracts.

  7. A Wind Accretion Model for HLX-1

    CERN Document Server

    Miller, M Coleman; Maccarone, Thomas J

    2014-01-01

    The brightest ultraluminous X-ray source currently known, HLX-1, has been observed to undergo five outburst cycles. The periodicity of these outbursts, and their high inferred maximum accretion rates of $\\sim{\\rm few}\\times 10^{-4} M_\\odot {\\rm yr}^{-1}$, naturally suggest Roche lobe overflow at the pericenter of an eccentric orbit. It is, however, difficult for the Roche lobe overflow model to explain the apparent trend of decreasing decay times over the different outbursts while the integrated luminosity also drops. Thus if the trend is real rather than simply being a reflection of the complex physics of accretion disks, a different scenario may be necessary. We present a speculative model in which, within the last decade, a high-mass giant star had most of its envelope tidally stripped by the $\\sim 10^{4-5} M_\\odot$ black hole in HLX-1, and the remaining core plus low-mass hydrogen envelope now feeds the hole with a strong wind. This model can explain the short decay time of the disk, and could explain the...

  8. Evaluation of new oxidation methods for the measurement of bilirubin on the aeroset clinical chemistry analyzer and comparison with methods on the Hitachi 717.

    Science.gov (United States)

    Sturm, Ernhard; Albrecht-Groos, Ragnhild; Seyfarth, Michael

    2002-01-01

    We evaluated analytical and performance quality of the new oxidation methods for direct and total bilirubin on the Abbott Aeroset clinical chemistry analyzer. Within-day imprecisions for Abbott Aeroset assays ranged from 0.7 to 2.9% and between-day imprecisions from 2.1 to 7.3%. Inaccuracies as compared with the control "target values" for the Jendrassik-Gróf method showed deviations of -18.2 to +4.2%. Limits of detection were determined and showed very low values of 300 micromol/l. A method comparison for 100 patient samples with established Jendrassik-Gróf and DPD methods on the Roche Hitachi 717 showed good linearities between the investigated methods (r > or = 0.995). Due to slopes that ranged from 0.829 to 0.950, reference ranges for the oxidation methods differ slightly from those of established Roche Jendrassik-Gróf methods, but results can be adapted by the introduction of converting factors. In conclusion, the oxidation bilirubin assays revealed convincing analytical and performance qualities for medical needs that were similar or even better than for established methods. Application of the oxidation methods on the Aeroset clinical chemistry analyzer also improves laboratory efficiency by increasing throughput, speed of obtaining results and lowered sample and reagent volumes compared to established methods.

  9. COUPLED EVOLUTIONS OF THE STELLAR OBLIQUITY, ORBITAL DISTANCE, AND PLANET'S RADIUS DUE TO THE OHMIC DISSIPATION INDUCED IN A DIAMAGNETIC HOT JUPITER AROUND A MAGNETIC T TAURI STAR

    International Nuclear Information System (INIS)

    We revisit the calculation of the ohmic dissipation in a hot Jupiter presented by Laine et al. by considering more realistic interior structures, stellar obliquity, and the resulting orbital evolution. In this simplified approach, the young hot Jupiter of one Jupiter mass is modeled as a diamagnetic sphere with a finite resistivity, orbiting across tilted stellar magnetic dipole fields in vacuum. Since the induced ohmic dissipation occurs mostly near the planet's surface, we find that the dissipation is unable to significantly expand the young hot Jupiter. Nevertheless, the planet inside a small corotation orbital radius can undergo orbital decay by the dissipation torque and finally overfill its Roche lobe during the T Tauri star phase. The stellar obliquity can evolve significantly if the magnetic dipole is parallel/antiparallel to the stellar spin. Our results are validated by the general torque-dissipation relation in the presence of the stellar obliquity. We also run the fiducial model of Laine et al. and find that the planet's radius is sustained at a nearly constant value by the ohmic heating, rather than being thermally expanded to the Roche radius as suggested by the authors.

  10. Performance evaluation of new automated hepatitis B viral markers in the clinical laboratory: two quantitative hepatitis B surface antigen assays and an HBV core-related antigen assay.

    Science.gov (United States)

    Park, Yongjung; Hong, Duck Jin; Shin, Saeam; Cho, Yonggeun; Kim, Hyon-Suk

    2012-05-01

    We evaluated quantitative hepatitis B surface antigen (qHBsAg) assays and a hepatitis B virus (HBV) core-related antigen (HBcrAg) assay. A total of 529 serum samples from patients with hepatitis B were tested. HBsAg levels were determined by using the Elecsys (Roche Diagnostics, Indianapolis, IN) and Architect (Abbott Laboratories, Abbott Park, IL) qHBsAg assays. HBcrAg was measured by using Lumipulse HBcrAg assay (Fujirebio, Tokyo, Japan). Serum aminotransferases and HBV DNA were respectively quantified by using the Hitachi 7600 analyzer (Hitachi High-Technologies, Tokyo, Japan) and the Cobas AmpliPrep/Cobas TaqMan test (Roche). Precision of the qHBsAg and HBcrAg assays was assessed, and linearity of the qHBsAg assays was verified. All assays showed good precision performance with coefficients of variation between 4.5% and 5.3% except for some levels. Both qHBsAg assays showed linearity from 0.1 to 12,000.0 IU/mL and correlated well (r = 0.9934). HBsAg levels correlated with HBV DNA (r = 0.3373) and with HBcrAg (r = 0.5164), and HBcrAg also correlated with HBV DNA (r = 0.5198; P HBcrAg assays.

  11. [Isolation and characterization of 27 polymorphic microsatellite markers in Armillaria luteo-virens ( Physalacriaceae)].

    Science.gov (United States)

    Xing, Rui; Gao, Qingbo; Zhang, Faqi; Li, Yinhu; Ful, Pengcheng; Zhang, Jinhua; Wang, Jiuli; Chen, Shilong

    2014-09-01

    [OBJECTIVE] The objectives of this study were to use Roche 454 GS FLX system to develop SSR markers for Armillaria luteo-virens. These datasets will be valuable for detecting genetic diversity and population structure of this species. [METHODS] We collected Armillaria luteo-virens samples from Yushu in Qinghai province, China. Total RNA was isolated by using the TRIzol reagent, after that we constructed cDNA library and performed one quarter plate of the whole run 454 pyrosequencing. We selected 98 primer pairs randomly from the 321 SSRs to evaluate their application and the polymorphism across 66 individuals (Armillaria luteo-virens) representing 3 wild populations. [RESULTS] Roche 454 sequencing yielded 197,121 reads with a total nucleotide size of 88,585,965bp. 27 of 98 SSRs loci were polymorphic. Numbers of alleles (Na) ranged from 2 to 8. Expected heterozygosity (HE) ranged from less than 0. 001 to 0. 810 at locus ALV65, while observed heterozygosity (Ho) from 0 at loci AIV64 and AIV92 to 0. 900 at loci ALV8. We found no evidence of linkage disequiliburium, however 10 of 27 SSR markers showed significant deviation from Hardy-weinberg equilibrium. [CONCLUSION] These remaining 17 pairs of Armillaria luteo-virens SSR markers will be valuable for future research on detecting population structure and conservation of this species.

  12. A Neutron Star–White Dwarf Binary Model for Repeating Fast Radio Burst 121102

    Science.gov (United States)

    Gu, Wei-Min; Dong, Yi-Ze; Liu, Tong; Ma, Renyi; Wang, Junfeng

    2016-06-01

    We propose a compact binary model for the fast radio burst (FRB) repeaters, where the system consists of a magnetic white dwarf (WD) and a neutron star (NS) with strong bipolar magnetic fields. When the WD fills its Roche lobe, mass transfer will occur from the WD to the NS through the inner Lagrange point. The accreted magnetized materials may trigger magnetic reconnection when they approach the NS surface, and therefore the electrons can be accelerated to an ultra-relativistic speed. In this scenario, the curvature radiation of the electrons moving along the NS magnetic field lines can account for the characteristic frequency and the timescale of an FRB. Owing to the conservation of angular momentum, the WD may be kicked away after a burst, and the next burst may appear when the system becomes semi-detached again through the gravitational radiation. By comparing our analyses with the observations, we show that such an intermittent Roche-lobe overflow mechanism can be responsible for the observed repeating behavior of FRB 121102.

  13. A Binary Teetering on the Edge

    Science.gov (United States)

    Motl, P. M.; D'Souza, M. C. R.; Tohline, J. E.; Frank, J.

    2005-05-01

    We present a fully three-dimensional hydrodynamical simulation of Roche lobe overflow in a binary near the stability boundary. This boundary separates evolutionary branches that correspond to either an accelerating mass transfer rate leading eventually to merger through tidal instability or to a decaying mass transfer rate as the orbit expands. The binary begins with a mass ratio of 0.4 (ratio of donor to accretor mass) and is initially assumed to be rotating synchronously. We treat the stellar components as simple polytropic fluids characterized by a polytropic index, n = 3/2. As the donor overflows its Roche lobe, the mass transfer rate initially accelerates before stabilizing and eventually dropping over a timescale of tens of orbits. We also note that for this particular binary, the accretion stream impacts on the surface of the donor rather than forming an accretion disk. This simulation allows us to measure the efficiency with which the accretion stream spins up the accretor in this "direct impact" scenario and the degree to which angular momentum is transfered back to the binary orbit via the tidal field.

  14. Accuracy of capillary whole blood international normalized ratio on the CoaguChek S, CoaguChek XS, and i-STAT 1 point-of-care analyzers.

    Science.gov (United States)

    Karon, Brad S; McBane, Robert D; Chaudhry, Rajeev; Beyer, Lisa K; Santrach, Paula J

    2008-07-01

    We evaluated the accuracy of capillary whole blood international normalized ratio (INR) on the CoaguChek S (Roche Diagnostics, Indianapolis, IN), CoaguChek XS (Roche Diagnostics), and i-STAT 1 (i-STAT, East Windsor, NJ) point-of-care (POC) analyzers compared with venous plasma INRs determined by a reference laboratory method. Overall agreement between POC and laboratory plasma INR was very good, with median bias between capillary whole blood and laboratory plasma INRs varying from 0.0 to -0.2 INR units on all devices. More than 90% of results on the CoaguChek XS and i-STAT 1 and 88% of CoaguChek S results were within 0.4 INR units of the reference laboratory method. The CoaguChek XS and i-STAT 1 demonstrated greater accuracy than the CoaguChek S as measured by the number of results that differed by more than 0.5 INR units from the reference method. Median bias between CoaguChek S capillary whole blood and laboratory plasma INRs changed over time, demonstrating the need for ongoing quality assurance measures for POC INR programs.

  15. Wobbling and precessing jets from warped disks in binary systems

    CERN Document Server

    Sheikhnezami, Somayeh

    2015-01-01

    We present results of the first ever three-dimensional (3D) magnetohydrodynamic (MHD) simulations of the accretion-ejection structure. We investigate the 3D evolution of jets launched symmetrically from single stars but also jets from warped disks in binary systems. We have applied various model setups and tested them by simulating a stable and bipolar symmetric 3D structure from a single star-disk-jet system. Our reference simulation maintains a good axial symmetry and also a bipolar symmetry for more than 600 rotations of the inner disk confirming the quality of our model setup. We have then implemented a 3D gravitational potential (Roche potential) due to a companion star and run a variety of simulations with different binary separations and mass ratios. These simulations show typical 3D deviations from axial symmetry, such as jet inclination outside the Roche lobe or spiral arms forming in the accretion disk. In order to find indication for precession effects, we have also run an exemplary parameter setup...

  16. Read length versus depth of coverage for viral quasispecies reconstruction.

    Directory of Open Access Journals (Sweden)

    Osvaldo Zagordi

    Full Text Available Recent advancements of sequencing technology have opened up unprecedented opportunities in many application areas. Virus samples can now be sequenced efficiently with very deep coverage to infer the genetic diversity of the underlying virus populations. Several sequencing platforms with different underlying technologies and performance characteristics are available for viral diversity studies. Here, we investigate how the differences between two common platforms provided by 454/Roche and Illumina affect viral diversity estimation and the reconstruction of viral haplotypes. Using a mixture of ten HIV clones sequenced with both platforms and additional simulation experiments, we assessed the trade-off between sequencing coverage, read length, and error rate. For fixed costs, short Illumina reads can be generated at higher coverage and allow for detecting variants at lower frequencies. They can also be sufficient to assess the diversity of the sample if sequences are dissimilar enough, but, in general, assembly of full-length haplotypes is feasible only with the longer 454/Roche reads. The quantitative comparison highlights the advantages and disadvantages of both platforms and provides guidance for the design of viral diversity studies.

  17. Read length versus depth of coverage for viral quasispecies reconstruction.

    Science.gov (United States)

    Zagordi, Osvaldo; Däumer, Martin; Beisel, Christian; Beerenwinkel, Niko

    2012-01-01

    Recent advancements of sequencing technology have opened up unprecedented opportunities in many application areas. Virus samples can now be sequenced efficiently with very deep coverage to infer the genetic diversity of the underlying virus populations. Several sequencing platforms with different underlying technologies and performance characteristics are available for viral diversity studies. Here, we investigate how the differences between two common platforms provided by 454/Roche and Illumina affect viral diversity estimation and the reconstruction of viral haplotypes. Using a mixture of ten HIV clones sequenced with both platforms and additional simulation experiments, we assessed the trade-off between sequencing coverage, read length, and error rate. For fixed costs, short Illumina reads can be generated at higher coverage and allow for detecting variants at lower frequencies. They can also be sufficient to assess the diversity of the sample if sequences are dissimilar enough, but, in general, assembly of full-length haplotypes is feasible only with the longer 454/Roche reads. The quantitative comparison highlights the advantages and disadvantages of both platforms and provides guidance for the design of viral diversity studies.

  18. Formation of the Double Neutron Star System PSR J1930-1852

    Science.gov (United States)

    Shao, Yong; Li, Xiang-Dong

    2016-01-01

    The spin period (185 ms) and period derivative (1.8× {10}-17 {{s}} {{{s}}}-1) of the recently discovered double neutron star (DNS) system PSR J1930-1852 indicate that the pulsar was mildly recycled through the process of Roche-lobe overflow. This system has the longest orbital period (45 days) of the known DNS systems, and can be formed from a helium star-NS binary if the initial mass of the helium star was ≲ 4.0{M}⊙ ; otherwise, the helium star would never fill its Roche-lobe. At the moment of the supernova explosion, the mass of the helium star was ≲ 3.0{M}⊙ . We find that the probability distribution of the velocity kick imparted to the new-born neutron star has a maximum at about 30 {km} {{{s}}}-1 (and a tail up to 260 {km} {{{s}}}-1), indicating that this NS probably received a low kick velocity at birth.

  19. Erziehungstheorie, Literatur und Geschlecht Educational Theory, Literature, and Gender

    Directory of Open Access Journals (Sweden)

    Barbara Gribnitz

    2007-07-01

    Full Text Available Die Verfasserin stellt sich die Aufgabe, das „erkenntnistheoretische Potential des literarischen Textes als Quelle historisch-pädagogischer Forschung“ (S. 12 am Beispiel des Genre Bildungsroman herauszuarbeiten. Anhand zweier Romane, die weibliche Lebensläufe beschreiben (Sophie von La Roche: Die Geschichte des Fräuleins von Sternheim; Friederike Helene Unger: Julchen Grünthal, erweitert sie die Bildungsromandebatte in der Erziehungs- und Literaturwissenschaft um die Kategorie Geschlecht und bestimmt „den literarischen Text als privilegierten Ort dieser Auseinandersetzung“ (S. 40. Die Studie leidet unter Redundanz und oberflächlicher Lektüre der Texte.The author takes on the task of identifying the “epistemological potential of the literary text as source of historical-pedagogical research,” using the genre of the bildungsroman (12. Focusing on two novels that describe the course of female life histories (Sophie von La Roch: History of Lady Sophia Sternheim/Die Geschichte des Fräuleins von Sternheim; Friederike Helene Unger: Julchen Grünthal, she expands the debate on the bildungsroman in education and literary studies to include the categories of gender and identifies “the literary text as the privileged space for this conflict” (40. The study suffers under redundancy and superficial readings of the texts.

  20. Improved Efficiency and Reliability of NGS Amplicon Sequencing Data Analysis for Genetic Diagnostic Procedures Using AGSA Software

    Science.gov (United States)

    Poulet, Axel; Privat, Maud; Viala, Sandrine; Decousus, Stephanie; Perin, Axel; Lafarge, Laurence; Ollier, Marie; El Saghir, Nagi S.

    2016-01-01

    Screening for BRCA mutations in women with familial risk of breast or ovarian cancer is an ideal situation for high-throughput sequencing, providing large amounts of low cost data. However, 454, Roche, and Ion Torrent, Thermo Fisher, technologies produce homopolymer-associated indel errors, complicating their use in routine diagnostics. We developed software, named AGSA, which helps to detect false positive mutations in homopolymeric sequences. Seventy-two familial breast cancer cases were analysed in parallel by amplicon 454 pyrosequencing and Sanger dideoxy sequencing for genetic variations of the BRCA genes. All 565 variants detected by dideoxy sequencing were also detected by pyrosequencing. Furthermore, pyrosequencing detected 42 variants that were missed with Sanger technique. Six amplicons contained homopolymer tracts in the coding sequence that were systematically misread by the software supplied by Roche. Read data plotted as histograms by AGSA software aided the analysis considerably and allowed validation of the majority of homopolymers. As an optimisation, additional 250 patients were analysed using microfluidic amplification of regions of interest (Access Array Fluidigm) of the BRCA genes, followed by 454 sequencing and AGSA analysis. AGSA complements a complete line of high-throughput diagnostic sequence analysis, reducing time and costs while increasing reliability, notably for homopolymer tracts.

  1. Impact of a Binary System Common Envelope on Mass Transfer through the Inner Lagrange Point

    CERN Document Server

    Bisikalo, D V; Kuznetsov, O A; Chechetkin, V M

    1997-01-01

    Results of numerical simulations of the impact of a common envelope on the matter flow pattern near the outflowing component in a semidetached binary system are presented. Three-dimensional modeling of the matter transfer gas dynamics in a low-mass X-ray binary X1822-371 enable investigation of the structure of flows in the vicinity of the inner Lagrange point L1. Taking into account the common envelope of the system substantially changes the flow pattern near the Roche surface of the outflowing component. In a stationary regime, accretion of common envelope gas is observed over a significant fraction of the donor star's surface, which inhibits the flow of gas along the Roche surface to L1. The change in the flow pattern is particularly significant near L1, where the stream of common envelope gas strips matter off the stellar surface. This, in turn, significantly increases (by an order of magnitude) the gas flow from the donor surface in comparison with the estimates of standard models.

  2. The Effect of Tides on the Population of PN from Interacting Binaries

    CERN Document Server

    Madappatt, Niyas; Villaver, Eva

    2016-01-01

    We have used the tidal equations of Zahn to determine the maximum orbital distance at which companions are brought into Roche lobe contact with their giant primary, when the primary expands during the giant phases. This is a key step when determining the rates of interaction between giants and their companions. Our stellar structure calculations are presented as maximum radii reached during the red and asymptotic giant branch (RGB and AGB, respectively) stages of evolution for masses between 0.8 and 4.0 Mo (Z=0.001 - 0.04) and compared with other models to gauge the uncertainty on radii deriving from details of these calculations. We find overall tidal capture distances that are typically 1-4 times the maximum radial extent of the giant star, where companions are in the mass range from 1 Jupiter mass to a mass slightly smaller than the mass of the primary. We find that only companions at initial orbital separations between ~320 and ~630 Ro will be typically captured into a Roche lobe-filling interaction or a ...

  3. Automated extraction improves multiplex molecular detection of infection in septic patients.

    Directory of Open Access Journals (Sweden)

    Benito J Regueiro

    Full Text Available Sepsis is one of the leading causes of morbidity and mortality in hospitalized patients worldwide. Molecular technologies for rapid detection of microorganisms in patients with sepsis have only recently become available. LightCycler SeptiFast test M(grade (Roche Diagnostics GmbH is a multiplex PCR analysis able to detect DNA of the 25 most frequent pathogens in bloodstream infections. The time and labor saved while avoiding excessive laboratory manipulation is the rationale for selecting the automated MagNA Pure compact nucleic acid isolation kit-I (Roche Applied Science, GmbH as an alternative to conventional SeptiFast extraction. For the purposes of this study, we evaluate extraction in order to demonstrate the feasibility of automation. Finally, a prospective observational study was done using 106 clinical samples obtained from 76 patients in our ICU. Both extraction methods were used in parallel to test the samples. When molecular detection test results using both manual and automated extraction were compared with the data from blood cultures obtained at the same time, the results show that SeptiFast with the alternative MagNA Pure compact extraction not only shortens the complete workflow to 3.57 hrs., but also increases sensitivity of the molecular assay for detecting infection as defined by positive blood culture confirmation.

  4. Constraints on Common Envelope Magnetic Fields from Observations of Jets in Planetary Nebulae

    CERN Document Server

    Tocknell, James; Wardle, Mark

    2013-01-01

    The common envelope (CE) interaction describes the swallowing of a nearby companion by a growing, evolving star. CEs that take place during the asymptotic giant branch phase of the primary and may lead to the formation of a planetary nebula (PN) with a post-CE close binary in the middle. We have used published observations of masses and kinematics of jets in four post-CE PN to infer physical characteristics of the CE interaction. In three of the four systems studied, Abell 63, ETHOS 1 and the Necklace PN, the kinematics indicate that the jets were launched a few thousand years before the CE and we favour a scenario where this happened before Roche lobe overflow, although better models of wind accretion and wind Roche lobe overflow are needed. The magnetic fields inferred to launch pre-CE jets are of the order of a few Gauss. In the fourth case, NGC 6778, the kinematics indicate that the jets were launched about 3000 years after the CE interaction. Magnetic fields of the order of a few hundreds to a few thousa...

  5. Risk Factors for Acquisition and Clearance of Oral Human Papillomavirus Infection Among HIV-Infected and HIV-Uninfected Adults

    Science.gov (United States)

    Beachler, Daniel C.; Sugar, Elizabeth A.; Margolick, Joseph B.; Weber, Kathleen M.; Strickler, Howard D.; Wiley, Dorothy J.; Cranston, Ross D.; Burk, Robert D.; Minkoff, Howard; Reddy, Susheel; Xiao, Weihong; Guo, Yingshi; Gillison, Maura L.; D'Souza, Gypsyamber

    2015-01-01

    Human papillomavirus (HPV) causes the majority of oropharyngeal cancers in the United States, yet the risk factors for and natural history of oral HPV infection are largely unknown. In 2010–2011, a US-based longitudinal cohort study of 761 human immunodeficiency virus (HIV)-infected and 469 at-risk HIV-uninfected participants from the Multicenter AIDS Cohort Study and the Women's Interagency HIV Study was initiated. Semiannually collected oral rinses were evaluated for 37 HPV genotypes using the Roche LINEAR ARRAY HPV Genotyping Test (Roche Molecular Systems, Pleasanton, California), and factors associated with oral HPV incidence and clearance were explored using adjusted Wei-Lin-Weissfeld modeling. Through 2013, the 2-year cumulative incidence of any type of oral HPV infection was 34% in HIV-infected persons and 19% in HIV-uninfected persons. However, many of these infections cleared. Seven percent of incident infections and 35% of prevalent infections persisted for at least 2 years. After adjustment for other risk factors, HIV infection (adjusted hazard ratio = 2.3, 95% confidence interval: 1.7, 3.2), reduced current CD4 cell count, and increased numbers of oral sex and “rimming” partners increased the risk of incident oral HPV infection, whereas male sex, older age, and current smoking increased the risk of oral HPV persistence (each P < 0.05). This helps explain the consistent associations observed between these factors and prevalent oral HPV infection in previous cross-sectional studies. PMID:25480823

  6. Trends, prospects and challenges in quantifying flow and transport through fractured rocks

    Science.gov (United States)

    Neuman, Shlomo P.

    2005-03-01

    of these trends in light of recent experimental and theoretical findings, ending with a short list of prospects and challenges for the future. Parmis les problèmes aucquels font face les hydrogéologues, il y a celui de la caractérisation des roches fracturées (Faybishenko and Benson, 2000). Cet article discute des solutions associées aux quantifications des écoulements et des transports à travers les roches fracturées à l'échelles des essais de puits et des essais de traçage. Une part importante de la litérature traite des roches cristallines, des roches sédimentaires consolidées telles les grés, les calcaires (exeptés les karsts) et la craie. De fait, cet article traitera la même panoplie de roches. La quantification directe des écoulements et du transport dans de tels milieux est généralement abordée via la géométrie des fractures, les données de pression et de traçage, qui déslors sont les objets principaux de notre étude. La géologie, la géophysique et la géochimie (incluant la géochimie isotopique) sont criticables car elles donnent des informations qualitatives sur l'écoulement et le transport des aquifères fracturés, et son intégrées graduellement dans des modèles qualitatifs. La manière d'intégrer ces données dépasse malheureusement cet article. L'hydrogéologie des aquifères de fractures et les autres sciences de la terre s'intéressant aux roches fracturées méritent des traîtements différents. En toute évidence il est suggéré que rarement un modèle d'écoulement et de transport dans une roche fracturée puisse être traité comme un milieu isotropique continu, uniforme ou moyennement non-uniforme. Par ailleurs, il est admis la dépendance entre la forte hétérogénéité erratique et la variété de natures et de comportements des roches fracturées. Une manière de régler le problème est de considérer le milieu comme un réseau de fractures discrètes (avec une perméabilité de matrice ou de bloc). Une

  7. RY Aquarius a Binary System with Pulsating delta-scuti Primary Component

    Science.gov (United States)

    Manzoori, Davood; Salar, Abbasvand

    2016-07-01

    We present simultaneous new BVI light curves along with radial velocity curve analysis of the RY Aqr system, using the PHysics Of Eclipsing BinariEs code. The analysis indicates that while the primary is completely inside its Roche critical surface, the secondary has filled out its Roche surface. In addition, the positions of the system components on M–R, H–R diagrams are specified, which show that the primary is a main-sequence or nearly main-sequence star while the secondary is an evolved subgiant. In addition, analysis of the period and luminosity variations of the system were carried out. Fourier frequency analysis of light variation indicates that the primary is a pulsating, δ-scuti variable star. Moreover, O–C curve analysis shows that the period of the system is secularly decreasing with a rate of dp/dt = 0.074 s yr‑1. This decrease in the orbital period variations was attributed to a mass and angular momentum loss from the system with a rate of 2.57× {10}-10{M}ȯ {{yr}}-1. Apart from the secular period decreases, the orbital period of the system is modulated by a cyclic period of 72.69 year, which was attributed to a third body orbiting around the barycenter of the system.

  8. THE HABITABILITY AND DETECTION OF EARTH-LIKE PLANETS ORBITING COOL WHITE DWARFS

    Energy Technology Data Exchange (ETDEWEB)

    Fossati, L.; Haswell, C. A.; Patel, M. R.; Busuttil, R. [Department of Physical Sciences, Open University, Walton Hall, Milton Keynes MK7 6AA (United Kingdom); Bagnulo, S. [Armagh Observatory, College Hill, Armagh BT61 9DG (United Kingdom); Kowalski, P. M. [GFZ German Research Centre for Geosciences, Telegrafenberg, D-14473 Potsdam (Germany); Shulyak, D. V. [Institute of Astrophysics, Georg-August-University, Friedrich-Hund-Platz 1, D-37077 Goettingen (Germany); Sterzik, M. F., E-mail: l.fossati@open.ac.uk, E-mail: C.A.Haswell@open.ac.uk, E-mail: M.R.Patel@open.ac.uk, E-mail: r.busuttil@open.ac.uk, E-mail: sba@arm.ac.uk, E-mail: kowalski@gfz-potsdam.de, E-mail: denis.shulyak@gmail.com, E-mail: msterzik@eso.org [European Southern Observatory, Casilla 19001, Santiago 19 (Chile)

    2012-09-20

    Since there are several ways planets can survive the giant phase of the host star, we examine the habitability and detection of planets orbiting white dwarfs. As a white dwarf cools from 6000 K to 4000 K, a planet orbiting at 0.01 AU would remain in the continuous habitable zone (CHZ) for {approx}8 Gyr. We show that photosynthetic processes can be sustained on such planets. The DNA-weighted UV radiation dose for an Earth-like planet in the CHZ is less than the maxima encountered on Earth, and hence non-magnetic white dwarfs are compatible with the persistence of complex life. Polarization due to a terrestrial planet in the CHZ of a cool white dwarf (CWD) is 10{sup 2} (10{sup 4}) times larger than it would be in the habitable zone of a typical M-dwarf (Sun-like star). Polarimetry is thus a viable way to detect close-in rocky planets around white dwarfs. Multi-band polarimetry would also allow us to reveal the presence of a planet atmosphere, providing a first characterization. Planets in the CHZ of a 0.6 M{sub Sun} white dwarf will be distorted by Roche geometry, and a Kepler-11d analog would overfill its Roche lobe. With current facilities a super-Earth-sized atmosphereless planet is detectable with polarimetry around the brightest known CWD. Planned future facilities render smaller planets detectable, in particular by increasing the instrumental sensitivity in the blue.

  9. Genome sequencing and analysis of a granulovirus isolated from the Asiatic rice leafroller, Cnaphalocrocis medinalis.

    Science.gov (United States)

    Zhang, Shan; Zhu, Zheng; Sun, Shifeng; Chen, Qijin; Deng, Fei; Yang, Kai

    2015-12-01

    The complete genome of Cnaphalocrocis medinalis granulovirus (CnmeGV) from a serious migratory rice pest, Cnaphalocrocis medinalis (Lepidoptera: Pyralidae), was sequenced using the Roche 454 Genome Sequencer FLX system (GS FLX) with shotgun strategy and assembled by Roche GS De Novo assembler software. Its circular double-stranded genome is 111,246 bp in size with a high A+T content of 64.8% and codes for 118 putative open reading frames (ORFs). It contains 37 conserved baculovirus core ORFs, 13 unique ORFs, 26 ORFs that were found in all Lepidoptera baculoviruses and 42 common ORFs. The analysis of nucleotide sequence repeats revealed that the CnmeGV genome differs from the rest of sequenced GVs by a 23 kb and a 17kb gene block inversions, and does not contain any typical homologous region (hr) except for a region of non-hr-like sequence. Chitinase and cathepsin genes, which are reported to have major roles in the liquefaction of the hosts, were not found in the CnmeGV genome, which explains why CnmeGV infected insects do not show the phenotype of typical liquefaction. Phylogenetic analysis, based on the 37 core baculovirus genes, indicates that CnmeGV is closely related to Adoxophyes orana granulovirus. The genome analysis would contribute to the functional research of CnmeGV, and would benefit to the utilization of CnmeGV as pest control reagent for rice production. PMID:26712716

  10. Mass Transfer from Giant Donors

    CERN Document Server

    Pavlovskii, K

    2014-01-01

    The stability of mass transfer in binaries with convective giant donors remains an open question in modern astrophysics. There is a significant discrepancy between what the existing methods predict for a response to mass loss of the giant itself, as well as for the mass transfer rate during the Roche lobe overflow. Here we show that the recombination energy in the superadiabatic layer plays an important and hitherto unaccounted-for role in he donor's response to mass loss, in particular on its luminosity and effective temperature. Our improved optically thick nozzle method to calculate the mass transfer rate via $L_1$ allows us to evolve binary systems for a substantial Roche lobe overflow. We propose a new, strengthened criterion for the mass transfer instability, basing it on whether the donor experiences overflow through its outer Lagrangian point. We find that with the new criterion, if the donor has a well-developed outer convective envelope, the critical initial mass ratio for which a binary would evolv...

  11. 两套不同检测系统甲状腺激素测定结果的可比性分析%The research on the comparability of thyroid hormone test results using two different detection systems

    Institute of Scientific and Technical Information of China (English)

    王建琼; 牛华; 陈玲; 张芹; 平竹仙

    2014-01-01

    目的:对Abbott Architect ci16200全自动生化免疫分析仪(雅培系统)和Roche Cobas e601全自动免疫分析仪(罗氏系统)检测甲状腺激素的结果进行对比分析。方法分别采用雅培系统[化学发光微粒子免疫分析(CM IA )]和罗氏系统[电化学发光免疫分析(ECLIA)]对93例血清标本进行甲状腺激素[甲状腺素(T4)、游离甲状腺素(FT4)、三碘甲腺原氨酸(T3)、游离三碘甲腺原氨酸(FT3)、促甲状腺激素(TSH)]的检测,并对结果进行分析。结果两套检测系统检测T4、FT4、T3、FT3、TSH的结果具有良好相关性,相关系数分别为0.960、0.962、0.976、0.900、0.999( P<0.01),但两套检测系统测定结果的差异有统计学意义(P<0.05)。结论两套检测系统测定甲状腺激素的结果不可比。%Objective To analysis of the thyroid hormone test results of Abbott Architect ci16200 automatic biochemical-im-mune analyzer (Abbott system) and Roche Cobas e601 automated immunoassay analyzer (Roche system) .Methods The thyroid hormone(including T4 ,FT4 ,T3 ,FT3 ,TSH ) levels of 93 cases of serum samples were respectively detected by Abbott system (CMIA) and Roche system (ECLIA) ,and the results were analyzed .Results The thyroid hormone test results of the two systems had a good correlation ,and the coefficients of T4 ,FT4 ,T3 ,FT3 ,and TSH were 0 .960 ,0 .962 ,0 .976 ,0 .900 ,and 0 .999(P<0 .01) . However ,there were significant difference of the detection results of the two systems(P<0 .05) .Conclusion The thyroid hormone test results of Abbott system and Roche system are not comparable .

  12. Erlotinib: CP 358774, NSC 718781, OSI 774, R 1415.

    Science.gov (United States)

    2003-01-01

    Erlotinib [Tarceva, R 1415, CP 358774, OSI 774, NSC 718781] is a small molecular, once-a-day, orally active inhibitor of the epidermal growth factor receptor tyrosine kinase. This profile has been selected from R&D Insight, a pharmaceutical intelligence database produced by Adis International Ltd. It is one of a class of anticancer drugs that target the underlying molecular mechanism involving oncogenes and tumour suppressor genes, which play critical roles in the conversion of normal cells into a cancerous state. Erlotinib is undergoing clinical development as an oral tablet by an alliance between OSI Pharmaceuticals, Genentech and Roche. OSI Pharmaceuticals, Genentech and Roche have entered an agreement for the global development and commercialisation of erlotinib. Under the terms of the agreement, Genentech and OSI will share costs and profit-taking for commercialising the product in the US. The overall costs of the development programme will be shared equally between the three companies. OSI will keep certain co-promotion rights in the US and Genentech will be responsible for commercialising the drug in the US should the FDA approve it. Roche will take the responsibility for obtaining regulatory approval and commercialisation in territories outside the US and pay royalties to OSI on net sales of the product in these markets. Initially, the alliance partners intend to pursue development of erlotinib in all the major tumour markets, particularly for non-small cell lung cancer (NSCLC) in which the group will focus on front-line combination approaches. Pfizer and OSI Pharmaceuticals in the US were developing erlotinib as a treatment for solid tumours. However, in June 2000, Pfizer merged with Warner-Lambert. The resulting company retained the Pfizer name, but in order to meet Federal Trade Commission requirements for the merger Pfizer granted all developmental and marketing rights for erlotinib to OSI Pharmaceuticals. This divestiture of the erlotinib portfolio, in

  13. Community risk factors for ocular Chlamydia infection in Niger: pre-treatment results from a cluster-randomized trachoma trial.

    Directory of Open Access Journals (Sweden)

    Abdou Amza

    Full Text Available BACKGROUND: Trachoma control programs utilize mass azithromycin distributions to treat ocular Chlamydia trachomatis as part of an effort to eliminate this disease world-wide. But it remains unclear what the community-level risk factors are for infection. METHODS: This cluster-randomized, controlled trial entered 48 randomly selected communities in a 2×2 factorial design evaluating the effect of different treatment frequencies and treatment coverage levels. A pretreatment census and examination established the prevalence of risk factors for clinical trachoma and ocular chlamydia infection including years of education of household head, distance to primary water source, presence of household latrine, and facial cleanliness (ocular discharge, nasal discharge, and presence of facial flies. Univariate and multivariate associations were tested using linear regression and Bayes model averaging. FINDINGS: There were a total of 24,536 participants (4,484 children aged 0-5 years in 6,235 households in the study. Before treatment in May to July 2010, the community-level prevalence of active trachoma (TF or TI utilizing the World Health Organization [WHO] grading system was 26.0% (95% CI: 21.9% to 30.0% and the mean community-level prevalence of chlamydia infection by Amplicor PCR was 20.7% (95% CI: 16.5% to 24.9% in children aged 0-5 years. Univariate analysis showed that nasal discharge (0.29, 95% CI: 0.04 to 0.54; P = 0.03, presence of flies on the face (0.40, 95% CI: 0.17 to 0.64; P = 0.001, and years of formal education completed by the head of household (0.07, 95% CI: 0.07 to 0.13; P = 0.03 were independent risk factors for chlamydia infection. In multivariate analysis, facial flies (0.26, 95% CI: 0.02 to 0.49; P = 0.03 and years of formal education completed by the head of household (0.06, 95% CI: 0.008 to 0.11; P = 0.02 were associated risk factors for ocular chlamydial infection. INTERPRETATION: We have found that the presence

  14. Co-infection of SENV-D among chronic hepatitis C patients treated with combination therapy with high-dose interferon-alfa and ribavirin

    Institute of Scientific and Technical Information of China (English)

    Chia-Yen Dai; Liang-Yen Wang; Ming-Lung Yu; Wan-Long Chuang; Wen-Yu Chang; Shinn-Cherng Chen; Li-Po Lee; Ming-Yen Hsieh; Nei-Jen Hou; Zu-Yau Lin; Ming-Yuh Hsieh

    2005-01-01

    AIM: The clinical significance of co-infection of SENV-D among patients with chronic hepatitis C (CHC) and response of both viruses to combination therapy with high-dose interferon-alfa (IFN) plus ribavirin remain uncertain and are being investigated.METHODS: Total 164 (97 males and 67 females, the mean age 48.1±11.4 years, range: 20-73 years, 128histologically proved) naive CHC patients were enrolled in this study. SENV-D DNA was tested by PCR method.Detection of serum HCV RNA was performed using a standardized automated qualitative RT-PCR assay (COBAS AMPLICOR HCV Test, version 2.0). HCV genotypes 1a,1b, 2a, 2b, and 3a were determined by using genotypespecific primers. Pretreatment HCV RNA levels were determined by using the branched DNA assay (Quantiplex HCV RNA 3.0). There are 156 patients receiving combination therapy with IFN 6 MU plus ribavirin for 24 wk and the response to therapy is determined.RESULTS: Sixty-one (37.2%) patients were positive for SENV-D DNA and had higher mean age than those who were negative (50.7±10.6 years vs46.6±11.6 years,P = 0.026). The rate of sustained viral response (SVR)for HCV and SENV-D were 67.3% (105/156) and 56.3%(27/48), respectively. By univariate analysis, the higher rate of SVR was significantly related to HCV genotype non-1b (P<0.001), younger ages (P = 0.014), lower pretreatment levels of HCV RNA (P = 0.019) and higher histological activity index (HAI) score for intralobular regeneration and focal necrosis (P = 0.037). By multivariate analyses, HCV genotype non-1b, younger age and lower pretreatment HCV RNA levels were significantly associated with HCV SVR (odds ratio (OR)/95% confidence interval (CI): 12.098/0.02-0.19, 0.936/0.890-0.998, and 3.131/1.080-9.077, respectively). The SVR of SENV-D was higher among patients clearing SENV-D than those who had viremia at the end of therapy (P = 0.04).CONCLUSION: Coexistent SENV-D infection, apparently associated with higher ages, is found in more than onethird Taiwanese

  15. Diagnostic value of nine nucleic acid amplification test systems for Mycobacterium tuberculosis complex

    Directory of Open Access Journals (Sweden)

    Gülnur Tarhan

    2015-09-01

    Full Text Available Objective: In this study, nine commercial Nucleic Acid Amplification Test Systems (NAATs were evaluated for diagnostic performance of Mycobacterium tuberculosis complex (MTBC from smear positive sputum species (SPss and smear negative sputum specimens (SNss. Methods: Sixty SPss and 55 SNss were examined icroscopically by Ehrlich Ziehl Neelsen (EZN staining method, and also inoculated on Löwenstein Jensen (LJ medium for culture. The sensitivity and specificity of nine NAATs were calculated according to LJ culture method accepted as gold standard. Results: When LJ culture results were taken as gold standard; the sensitivity rates of method COBAS Amplicor MTB (Method A, GenProbe MTD (Method B, Cobas TaqMan MTB PCR Method C, iCycler iQ RT PCR (Method D, TaqMan PCR AB 5700 (Method E, TaqMan PCR AB7700 (Method F, ightCycler® 480 RT PCR (Method G, Rotor Gene RT PCR (Method H and the AdvanSure TB/NTM RT PCR (Method I for SPss were 98.3 %, 93.3 %, 96.7 %, 100 %, 93.3 %, 100 %, 100 %, 100 % and 100 %, respectively. The sensitivity was 53.84% for the methods A, B, D, E, G and I; 38.46% for the method C and H; 61.5% for the method F for the method I in SNss. There were no statistical significant differences between the nine NAATs (p≥0.05. The specificity was 100% for all nine NAATs in SNss. The positivity rates of methods were 53.8% for methods A, B, D, E, G, I; 38.5% for methods C and H, and 61.5% for method F in SNss. These rates were 100% for D, F, G, H and I; 98.3% for method A; 96.7% for method C; 93,3% for methods B and E in SPss. Statistical analysis showed that there was no statistically significant differences among the nine NAATs (p≥0.05. Conclusion: It is concluded that the nine NAATs might be useful for detecting MTBC from SPss, but not effective for SNss. J Microbiol Infect Dis 2015;5(3: 103-109

  16. Evaluación de dos estrategias de pigmentación en salmón coho (Oncorhynchus kisutch Evaluation of two pigmentation strategies in coho salmon (Oncorhynchus kisutch

    Directory of Open Access Journals (Sweden)

    J Pokniak

    2005-01-01

    Full Text Available El objetivo del presente trabajo fue evaluar el efecto del sexo y de dos tiempos de incorporación de astaxantina (AXT en la dieta sobre indicadores productivos, la coloración y pigmentación del salmón coho (Oncorhynchus kisutch a la cosecha. La experiencia se efectuó en balsas jaula (5x10x10 con 1368 salmones coho distribuidos al azar en cuatro jaulas con 342 peces cada una, asignados a dos tratamientos: control (80 ppm AXT por 105 días y experimental (80 ppm AXT por 53 días. Las características controladas fueron: peso, factor de condición, rendimiento de la canal, índices hepatosomático y gonadosomático, color y pigmentación del filete y steak. El grado de coloración se evaluó con Carta Color Roche®, Abanico Colorimétrico Salmo-Fan Roche® y Minolta Chroma Meter CR-200. La pigmentación en steaks y filete se registró con NIR (Near Infrared Reflectance. La respuesta productiva fue similar para ambos tratamientos, destacó un importante crecimiento gonadal en ambos tratamientos, lo que se reflejó en el alto índice gonadosomático alcanzado, superior a 5,5%. Las correlaciones encontradas fueron bajas y presentaron distintos niveles de significación. La coloración y pigmentación de steaks y filetes fueron mayores (P £ 0,05 para el tratamiento que recibió pigmento por 105 días. El efecto sexo originó diferencias significativas tanto en steaks como en filetes a favor de los machos. Los filetes de ambos tratamientos lograron la coloración mínima comercialmente exigida para ese corte, correspondiente a 15 según Carta Color Roche®, pero aquéllos del tratamiento experimental lograron un valor bajo el mínimo exigido con el abanico colorimétrico Roche®. De acuerdo a las condiciones de este ensayo los salmones coho alimentados con una dieta pigmentada por un periodo de 53 días no fue suficiente para lograr una apropiada coloración de sus filetes, cuando la evaluación se efectúa con una pauta más refinada como el

  17. Shear-Wave Splitting in a Critical Crust: the Next Step Biréfringence des ondes transversales dans les croûtes critiques : la prochaine étape

    Directory of Open Access Journals (Sweden)

    Crampin S.

    2006-12-01

    -organized criticality. The next step is to employ these techniques to model, monitor, and predict the effects of changing conditions on the deformation of the rockmass. On pourrait avancer que l'anisotropie dans la biréfringence des ondes transversales n'a pas répondu à ses promesses initiales, à savoir ouvrir une nouvelle voie dans la compréhension des phénomènes de fissures et de contraintes dans la croûte terrestre. Dans cet article sont présentés deux développements révélés récemment, qui paraissent raviver ces premiers espoirs et apportent des opportunités nouvelles pour le contrôle, la modélisation et même la prévision des déformations (avant fracture dans les roches microfracturées et saturées de fluides. Ainsi, un modèle de poroélasticité (APE développé récemment concerne l'évolution sous contraintes des roches microfracturées et saturées en fluide et reproduit une large gamme de phénomènes, qui seraient autrement inexpliqués ou dissociés, et semble être une bonne approximation au premier ordre de l'évolution des roches microfracturées et saturées en fluide. Puisque les paramètres qui contrôlent à petite échelle la déformation (avant fracture contrôlent aussi la biréfringence des ondes transversales, il apparaît que l'évolution des roches microfracturées et saturées peut être aussi directement contrôlée par cette biréfringence et que la réponse à des changements futurs peut être prédite par l'APE. Le bon usage de la modélisation de l'APE et des observations de la biréfringence des ondes transversales implique que la plupart des roches soient proches d'un stade de fracturation critique associé à une percolation limite, situation où la résistance aux contraintes transversales disparaît et où les fractures transversales peuvent se propager. Ceci corrobore une autre hypothèse concernant la mise en situation critique spontanée des roches in situ. La conséquence de cette identification est que la physique à petite

  18. 西安地区健康人群ALT与AST参考区间的初步探讨%Study the Reference Intervals for the Hepatic Marker Enzymes ALT and AST

    Institute of Scientific and Technical Information of China (English)

    周铁成; 张莹; 童开; 岳乔红; 郝晓柯

    2011-01-01

    females. Activities of ALT and AST were measured distinguish with the Roche detection system with pyridoxal phosphate, the Roche detection system without pyridoxal phosphate and the wako detection system. To study the reference intervals for the hepatic marker enzymes ALT and AST in Xi'an. Results There were some difference between the three systems CP<0. 01). The ALT and AST activities were found to be statistically different between males and females. Therefore, gender-specific reference intervals were established as the Roche detection system with pyridoxal phosphate,ALT-P:10. 24-57. 06 U/L (F),11. 02-84. 00 U/L (M)I AST-Pi 13. 55~50.14 U/L (F),15. 3~53. 42 U/L (M). The Roche detection system without pyridoxal phosphate, ALT-1S7.46~51.34 U/L (F),9. 09-71. 06 U/L (M):AST-1:11. 89~40. 49 U/L (F),13. 6~40. 52 U/L (M).The Wako detection system ALT:6. 41 - 41. 73 U/L (F),7. 2167. 77 U/L (M); AST:9. 41 -40. 64 U/L (F),12.1-41. 11 U/L (M). Conclusion The reference upper limits of the two enzymes in Xi'an population compared with others similar studies are higher. There were some difference between the three systems (P<0. 01). The reference range of Roche reagent with pyridoxal phosphate was higher than the reference range of Roche reagent without pyridoxal phosphate. The former was 15% to 20% higher than the latter. But the reference range of Roche reagent without pyridoxal phosphate was higher than the reference range of Wako reagent without pyridoxal phosphate,the former was higher than the latter 10%. The reference range of Wako reagent without pyridoxal phosphate was higher than the reference range currently used in clinical.

  19. Comparison of serum HBsAg quantitation by four immunoassays, and relationships of HBsAg level with HBV replication and HBV genotypes.

    Directory of Open Access Journals (Sweden)

    Edouard Tuaillon

    Full Text Available BACKGROUND: The decline in hepatitis B virus surface antigen (HBsAg may be an early predictor of the viral efficacy of Hepatitis B virus (HBV therapy. The HBsAg levels obtained by different immunoassays now need comparing and the relationships between levels of HBsAg and HBV DNA alongside HBsAg and genotype must be evaluated. METHODOLOGY/PRINCIPAL FINDINGS: HBsAg levels were compared among 80 patients using the Abbott Architect assay, a commercial immunoassay approved for HBsAg detection and quantitation, and three other assays derived from immunoassays approved for HBsAg detection (manufactured by Diasorin, Bio-Rad and Roche. Good correlation was found between the Abbot vs. Diasorin, Bio-Rad and Roche assays with narrow 95% limits of agreement and small mean differences: -0.06 to 0.11, -0.09 log(10 IU/mL; -0.57 to 0.64, -0.04 log(10 IU/mL; -0.09 to 0.45, -0.27 log(10 IU/mL, respectively. These agreements were not affected by genotypes A or D. HBsAg was weakly correlated with HBV DNA, whatever the HBsAg assay used: Abbott, ρ = 0.36 p = 0.001, Diasorin ρ = 0.34, p = 0.002; Bio-Rad ρ = 0.37, p<0.001; or Roche ρ = 0.41, p<0.001. This relationship between levels of HBsAg and HBV DNA seemed to depend on genotypes. Whereas HBsAg (Abbott assay tended to correlate with HBV DNA for genotype A (ρ = 0.44, p = 0.02, no such correlation was significant for genotypes D (ρ = 0.29, p = 0.15. CONCLUSION/SIGNIFICANCE: The quantitation of HBsAg in routine clinical samples is comparable between the reference assay and the adapted assays with acceptable accuracy limits, low levels of variability and minimum discrepancy. While HBsAg quantitation is not affected by HBV genotype, the observed association between levels of HBsAg and HBV DNA seems genotype dependent.

  20. Performance of an Optimized Paper-Based Test for Rapid Visual Measurement of Alanine Aminotransferase (ALT in Fingerstick and Venipuncture Samples.

    Directory of Open Access Journals (Sweden)

    Sidhartha Jain

    Full Text Available A paper-based, multiplexed, microfluidic assay has been developed to visually measure alanine aminotransferase (ALT in a fingerstick sample, generating rapid, semi-quantitative results. Prior studies indicated a need for improved accuracy; the device was subsequently optimized using an FDA-approved automated platform (Abaxis Piccolo Xpress as a comparator. Here, we evaluated the performance of the optimized paper test for measurement of ALT in fingerstick blood and serum, as compared to Abaxis and Roche/Hitachi platforms. To evaluate feasibility of remote results interpretation, we also compared reading cell phone camera images of completed tests to reading the device in real time.96 ambulatory patients with varied baseline ALT concentration underwent fingerstick testing using the paper device; cell phone images of completed devices were taken and texted to a blinded off-site reader. Venipuncture serum was obtained from 93/96 participants for routine clinical testing (Roche/Hitachi; subsequently, 88/93 serum samples were captured and applied to paper and Abaxis platforms. Paper test and reference standard results were compared by Bland-Altman analysis.For serum, there was excellent agreement between paper test and Abaxis results, with negligible bias (+4.5 U/L. Abaxis results were systematically 8.6% lower than Roche/Hitachi results. ALT values in fingerstick samples tested on paper were systematically lower than values in paired serum tested on paper (bias -23.6 U/L or Abaxis (bias -18.4 U/L; a correction factor was developed for the paper device to match fingerstick blood to serum. Visual reads of cell phone images closely matched reads made in real time (bias +5.5 U/L.The paper ALT test is highly accurate for serum testing, matching the reference method against which it was optimized better than the reference methods matched each other. A systematic difference exists between ALT values in fingerstick and paired serum samples, and can be

  1. Photometric Properties for Selected Algol-type Binaries. II. AO Serpentis and V338 Herculis

    Science.gov (United States)

    Yang, Y.-G.; Hu, S.-M.; Guo, D.-F.; Wei, J.-Y.; Dai, H.-F.

    2010-04-01

    We present the first multiband photometry for the semidetached eclipsing binary AO Serpentis, observed on seven nights between 2009 April and July at the Weihai Observatory of Shandong University. By using the 2003 version of the Wilson-Devinney code, the photometric solutions of AO Ser and a similar object V338 Her were (re)deduced. The spectral types and orbital periods are A2 and P = 0.8793 days for AO Ser, F1V and P = 1.3057 days for V338 Her. The results reveal that two binaries are low mass ratio systems, whose secondary components fill their Roche lobes. The fill-out factors of the primary components are f = 58.6% for AO Ser and f = 54.2% for V338 Her, respectively. From the O - C curves of AO Ser and V338 Her, it is discovered that secular period changes with cyclic variations exist. The periods and semiamplitudes are 17.32(±0.01) yr and 0.0051(±0.0001) days for AO Ser, 29.07(±0.04) yr and 0.0116(±0.0015) days for V338 Her, respectively. This kind of cyclic oscillation may be attributed to either the light-time effect via an assumed third body or perhaps cyclic magnetic activity on the secondary component. For AO Ser, the long-term period decreases at a rate of dP/dt = -5.35(±0.03) × 10-7 days yr-1, which may be caused by mass and angular momentum loss from the system. Considering the period decreasing, the fill-out factor of the primary for AO Ser will increase and it will finally fill its Roche lobe. Meanwhile, the secular period increase rate for V338 Her is dP/dt = +1.44(±0.24) × 10-7 days yr-1, indicating that mass transfers from the less massive component to the more massive component. This will also cause the fill-out factor of the primary to increase. When the primaries fill their Roche lobes, AO Ser and V338 Her may evolve into contact stars, as predicted by the theory of thermal relaxation oscillations.

  2. Evolution of the Northeastern Arabian Plate Margin and Shelf: Hydrocarbon Habitat and Conceptual Future Potential L'évolution géologique de la plate-forme arabe et de sa marge : répartition des hydrocarbures et concepts pour son potentiel futur

    Directory of Open Access Journals (Sweden)

    Beydoun Z. R.

    2006-11-01

    Full Text Available About 98% of the huge recoverable hydrocarbon reserves of the Arabian Plate are concentrated in the Northeastern Margin Shelf extending from NW Iraq through the Gulf to Central Oman. The plate tectonic evolution of this region is reviewed as background for its enormous hydrocarbon productivity. This is intimately linked to a near-continuous passive margin history and gentle subsidence of an extraordinarily wide epeiric shelf, which allowed repeated regional development of source rocks in large intra-shelf basins frequently in optimal juxtapositional relationships with good regional reservoirs and seals; these events are discussed. Although currently reported reserve figures relate to Mesozoic and Tertiary reservoirs, it is concluded that several pre-Mesozoic regional sources have generated enormous volumes of hydrocarbons, the bulk of which still await discovery in up-dip Paleozoic reservoirs, and that future ultimate recoverable reserves could conceivably double. Près de 98 % des énormes réserves récupérables d'hydrocarbures de la plaque arabique sont concentrés dans la marge nord-est qui s'étend du nord-ouest de l'Irak au centre d'Oman, à travers le Golfe. L'auteur passe en revue l'évolution tectonique de cette région, origine de son énorme productivité pétrolière. La passivité presque continue de cette marge, combinée à l'affaissement progressif d'un plateau épicontinental particulièrement large, a permis le développement local répété de roches mères, au sein du plateau, dans de vastes bassins bénéficiant souvent d'une juxtaposition optimale avec les roches réservoir et roche-couvertures. L'article étudie ces évènements. Si les réserves actuellement prises en compte sont essentiellement d'origine mésozoïque et tertiaire, l'auteur conclut que de nombreuses sources régionales pré-mésozoïques ont généré d'énormes volumes d'hydrocarbures, dont la majeur partie reste à découvrir dans des réservoirs pal

  3. Screening Brucella spp. in bovine raw milk by real-time quantitative PCR and conventional methods in a pilot region of vaccination, Edirne, Turkey.

    Science.gov (United States)

    Kaynak-Onurdag, F; Okten, S; Sen, B

    2016-05-01

    Brucellosis is a worldwide zoonotic disease transmitted to humans by consumption of contaminated milk and milk products. Brucellosis is endemic in Turkey, and Edirne has a high Brucella prevalence. Brucellosis is prevented by live-attenuated vaccines for animals and the vaccination program has been in place since 1984 in Turkey. Thrace is the pilot region for this vaccination program. The gold standard diagnostic technique for brucellosis is still the isolation of suspicious bacterial colonies followed by bacteriological identification, but it is very time consuming and laborious. In many studies, Brucella has been investigated by PCR techniques. However, PCR-based methods cannot differentiate between the vaccine strain and the virulent strain; thus, the vaccine strain may interfere with the virulent strain and causes false-positive reactions. To monitor brucellosis control programs effectively, it is important to distinguish vaccine and field strains of Brucella spp. In this study, raw milk samples were collected from 99 cows at 12 different barns in 5 villages of Edirne (Turkey). Bacteriological analyses and real-time quantitative (q)PCR experiments were applied to all samples. The DNA was isolated using Biospeedy DNA-Tricky Purification Kit (Bioeksen, Istanbul, Turkey). For all reactions, Roche Light Cycler Nano (Roche Diagnostics, Mannheim, Germany) instrument and Biospeedy EvaGreen qPCR Pre-Mix (Bioeksen) were used. The data were analyzed using Roche LightCycler NanoSoftware 1.0. For samples that were negative by bacteriological analyses and positive by qPCR, we developed a novel qPCR-based method to differentiate the virulent B. abortus strains and B. abortus S19 vaccine strain. We designed qPCR primers targeting the outer membrane protein of B. abortus. The qPCR products were sequenced using the ABI Prism Big Dye Terminator Cycle Sequencing Ready Reaction Kit on an ABI Prism 377 DNA sequencer (Applied Biosystems, Foster City, CA). In total, 2.02% of the

  4. Detection of Staphylococcus aureus enterotoxin production genes from patient samples using an automated extraction platform and multiplex real-time PCR.

    Science.gov (United States)

    Chiefari, Amy K; Perry, Michael J; Kelly-Cirino, Cassandra; Egan, Christina T

    2015-12-01

    To minimize specimen volume, handling and testing time, we have developed two TaqMan(®) multiplex real-time PCR (rtPCR) assays to detect staphylococcal enterotoxins A-E and Toxic Shock Syndrome Toxin production genes directly from clinical patient stool specimens utilizing a novel lysis extraction process in parallel with the Roche MagNA Pure Compact. These assays are specific, sensitive and reliable for the detection of the staphylococcal enterotoxin encoding genes and the tst1 gene from known toxin producing strains of Staphylococcus aureus. Specificity was determined by testing a total of 47 microorganism strains, including 8 previously characterized staphylococcal enterotoxin producing strains against each rtPCR target. Sensitivity for these assays range from 1 to 25 cfu per rtPCR reaction for cultured isolates and 8-20 cfu per rtPCR for the clinical stool matrix.

  5. Homotopical topology

    CERN Document Server

    Fomenko, Anatoly

    2016-01-01

    This classic text of the renowned Moscow mathematical school equips the aspiring mathematician with a solid grounding in the core of topology, from a homotopical perspective. Its comprehensiveness and depth of treatment are unmatched among topology textbooks: in addition to covering the basics—the fundamental notions and constructions of homotopy theory, covering spaces and the fundamental group, CW complexes, homology and cohomology, homological algebra—the book treats essential advanced topics, such as obstruction theory, characteristic classes, Steenrod squares, K-theory and cobordism theory, and, with distinctive thoroughness and lucidity, spectral sequences. The organization of the material around the major achievements of the golden era of topology—the Adams conjecture, Bott periodicity, the Hirzebruch–Riemann–Roch theorem, the Atiyah–Singer index theorem, to name a few—paints a clear picture of the canon of the subject. Grassmannians, loop spaces, and classical groups play a central role ...

  6. Population Synthesis for the Symbiotic Stars with Main-sequence Accretors

    Institute of Scientific and Technical Information of China (English)

    Guo-Liang Lü; Chun-Hua Zhu; Bin Wu; Zhan-Wen Han

    2006-01-01

    Using a population synthesis code, we have investigated the formation of symbiotic systems in which the hot component is a main-sequence star that is accreting matter from the cool component via Roche lobe overflow (RLOF). The RLOF can be divided into two cases:dynamically unstable and stable. In the first case, the birthrate of symbiotic stars is 0.056 yr-1or 0.045 yr-1 depending on different assumptions; in the stable RLOF case, it is 0.002 yr-1or 0.005 yr-1. The number of symbiotic stars with main-sequence accretors and unstable RLOF in our galaxy is about 5, that with stable RLOF is about 60 to 280. Comparison between our results with those of Yungelson et al. shows that symbiotic stars with MS accretors make only a small contribution ((<)8%) to the whole population of symbiotic stars in the Galaxy.

  7. Discovery of a Redback Millisecond Pulsar Candidate: 3FGL J0212.1+5320

    CERN Document Server

    Li, Kwan-Lok; Hou, Xian; Mao, Jirong; Strader, Jay; Chomiuk, Laura; Tremou, Evangelia

    2016-01-01

    We present a multi-wavelength study of the unidentified Fermi object, 3FGL J0212.1+5320. Within the 95% error ellipse, Chandra detects a bright X-ray source, which has a low-mass optical counterpart (M 64% of the Roche-lobe. Spectroscopic data taken in 2015 from the Lijiang observatory show no evidence of strong emission lines, revealing the accretion is currently inactive (the pulsar state). While the X-ray luminosity and the X-ray-to-gamma-ray flux ratio are both high that are comparable to that of the two known gamma-ray transitional millisecond pulsars, 3FGL J0212.1+5320 could be a promising target to search for future transition to the accretion active state.

  8. The elusive origin of Carbon-Enhanced Metal-Poor stars

    CERN Document Server

    Abate, C; Izzard, R G; Mohamed, S S; de Mink, S E

    2013-01-01

    Carbon-enhanced metal-poor (CEMP) stars count for 9-25% of all the very metal-poor stars of the halo. In at least some CEMP stars the chemical enrichment is believed to be due to wind mass transfer in the past from an AGB donor star on to a low-mass companion. However, binary population synthesis models predict much lower CEMP fractions. As an alternative to the canonical Bondi-Hoyle-Lyttleton (BHL) wind accretion model, recent hydrodynamical simulations suggest an efficient mode of wind mass transfer, called wind Roche-lobe overflow (WRLOF), can reproduce observations of AGB winds in binary systems. We use our population synthesis model to test the consequences of WRLOF on a population of CEMP stars. Compared to previous predictions based on the BHL model we find a modest increase of the fraction of CEMP stars and substantial differences in the distributions of carbon and periods in the population of CEMP stars.

  9. Urinary microalbumin measurement using a homogeneous liposomal immunoassay.

    Science.gov (United States)

    Frost, S J; Chakraborty, J; Firth, G B

    1996-08-14

    A homogeneous colorimetric immunoassay which has been developed for urinary microalbumin utilizes complement-mediated immunolysis of liposomes containing the dye, sulphorhodamine B. Unlike a previously described model complement-mediated liposomal assay for serum albumin (Frost et al., 1994) which was competitive, this assay uses a sandwich-type format and Fab' (antialbumin)-coated liposomes to increase the assay sensitivity. The liposomal assay, performed using a Cobas Bio analyser (Roche, Welwyn Garden City, UK), gave an acceptable correlation with a radioimmunoassay (NETRIA, London, UK): r = 0.94; y (liposomal assay) = 1.09 x (radioimmunoassay) - 1.54 mg/1. The imprecisions of the assays were similar and matrix effects due to the use of urine samples were determined to be acceptably small. The assay demonstrates the advantage of using Fab'-coated liposomes in sandwich-type liposomal immunoassays over liposomes coated with intact antibody, which failed to elicit complement-mediated immunolysis. PMID:8765163

  10. A soluble form of the transcobalamin receptor CD320 can be detected in human serum

    DEFF Research Database (Denmark)

    Arendt, Johan Frederik Berg; Quadros, Edward V.; Christensen, Anna Lisa;

    2010-01-01

    system. A medium containing recombinant CD320 (produced in-house) and commercially available CD320 (Abnova) were explored as suitable calibrators. Standard Western blot was performed to ensure the specificity of the capture antibody. Serum from patients referred for measurement of plasma Cbl(measured on...... was to establish an ELISA for sCD320 and to explore the occurrence of sCD320 in human serum. Methods: In order to develop an ELISA we used an in-house monoclonal antibody as capture antibody, and for detection, a biotin labelled polyclonal antibody (R&D Systems) combined with an avidin based detection...... Cobas 6000, Roche Diagnostics) and patients with extreme values of serum TC was analysed. Total TC was measured employing an in-house ELISA (2). Results: The Western blot showed a highly specific capture antibody. The ELISA assay showed a curvilinear response for dilutions of the medium/recombinant CD...

  11. Modeling of Multiphase with Respect to Low Interfacial Tension by Pseudo-Two-Phase Relative Permeability Functions Modélisation d'un écoulement polyphasique à faible tension interfaciale par des fonctions pseudo-biphasiques de perméabilité relative

    Directory of Open Access Journals (Sweden)

    Pusch G.

    2006-11-01

    Full Text Available A new 2-parameter desaturation function is introduced which offers a broader range of applicability to reservoir rock. Based on this function two-phase relative permeabilities are derived for oil phase and microemulsion flow. These functions are used to match a laboratory experiment by using surfactant flooding for a single surfactant system. Les auteurs présentent une nouvelle fonction de désaturation à deux paramètres qui offre une plus large gamme de possibilités d'application aux roches réservoir. On tire de cette fonction des perméabilités relatives biphasiques pour l'écoulement de la phase pétrole et d'une microémulsion. Ces fonctions sont utilisées pour reproduire une expérience de laboratoire avec injection de surfactant pour un seul système surfactant.

  12. Direct image for multiplicative and relative $K$-theories from transgression of the families index theorem, part 2

    CERN Document Server

    Berthomieu, Alain

    2007-01-01

    This is the sequel of the first part math.DG/0611281. Here, the procedure of transgressing the families index theorem (the so-called $\\eta$-form) is adapted to take in account the case of Dirac type operators with kernels of varying dimension. The constructed form is then used to define the direct image under proper submersions of the ``free multiplicative'' $K$-theory which was defined in the first part, the behaviour of the characteristic classes on free multiplicative $K$-theory under submersion is studied. Some universal caracterisation of the forms is provided. Finally, combining our result with Bismut and Lott's results on direct images of flat vector bundles yields a ``Grothendieck-Riemann-Roch'' theorem for Nadel-Chern-Simons classes on relative $K$-theory for flat vector bundles which was defined in the first part.

  13. Apport de l'analyse interactive d'images à l'étude de l'évolution structurale de zones déformées : application à une zone de cisaillement pan-africaine de l'Adrar des Iforas (Mali) et aux orthogneiss du massif du Grand Paradis (Alpes, Italie)

    OpenAIRE

    Champenois, Michel

    1989-01-01

    L'analyse quantitative de la déformation des roches apparaît indispensable si l'on veut pouvoir comprendre et discuter les mécanismes de la déformation à différentes échelles d'observation. Dans ce mémoire, l'étude de la déformation finie à l'échelle microscopique, a été réalisée à partir de la forme et de l'orientation de cristaux constituant les populations de marqueurs. A cette fin, nous avons développé un outil permettant d'avoir facilement accès, aux paramètres de forme et d'orientation,...

  14. Conception d'une machine d'essai française pour caractériser les mécanismes de libération des fibres d'amiante dans l'air lors des chantiers de BTP. Action 5 convention DGPR 2010

    OpenAIRE

    MAURIN, Patrice; HUGUES, Laure; PEREZ, Jean-Louis

    2011-01-01

    l'analyse des différentes machines utilisées dans le monde pour caractériser le dégagement des fibres dans l'air montre qu'elles ne semblent pas adaptées au problème posé (roches amiantifères dures). C'est pourquoi, il est proposé de réaliser deux machines adaptées à partir des essais Los Angelès et Micro-Deval avec mesure du taux de dégagement des fibres d'amiante. La validation de ce protocole expérimental d'essais ferait l'objet d'un premier point d'un plan d'actions qui pourrait comporter...

  15. [Metagenomic Analysis of Microbial Communities of the Sediments of the Kara Sea Shelf and the Yenisei Bay].

    Science.gov (United States)

    Mamaeva, E V; Galach'yants, Yu P; Khabudaev, K V; Petrova, D P; Pogodaeva, T V; Khodzher, T B; Zemskaya, T I

    2016-01-01

    Microbial diversity in the sediments of the Kara Sea shelf and the southern Yenisei Bay, differing in pore water mineralization, was studied using massive parallel pyrosequencing according to the 454 (Roche) technology. Members of the same phyla (Cyanobacteria, Verrucomicrobia, Actinobacteria, Proteobacteria, and Bacteroidetes) predominated in bacterial communities of the sediments, while their ratio and taxonomic composition varied within the phyla and depended on pore water mineralization. Increasing salinity gradient was found to coincide with increased share of the γ-Proteobacteria and decreased abundance of α- and β-Proteo- bacteria, as well as of the phyla Verrucomicrobia, Chloroflexi, Chlorobi, and Acidobacteria. Archaeal diversity was lower, with Thaumarchaeota predominant in the sediments with high and low mineralization, while Crenarchaeota predominated in moderately mineralized sediments. Microbial communities of the Kara Sea shelf and Yenisei Gulf sediments were found to contain the organisms capable of utilization of a broad spectrum of carbon sources, including gaseous and petroleum hydrocarbons. PMID:27476207

  16. Introduction

    OpenAIRE

    Bonnenfant, Paul

    2014-01-01

    Les montagnes, cœur de l’Arabie Heureuse, lui donnent sa force et son caractère. Deux fois dans l’année, en mars-avril et en juillet-août, les nuées s’accumulent sur le haut pays et le tonnerre gronde. L’eau dissout les roches et de leur diversité engendre des terroirs fertiles. Vers l’est, elle part vers le grand désert d’Arabie, en de vastes vallées où l’eau tantôt coule sous les sables, tantôt s’épanche en crues subites et redoutables. Le royaume sabéen avait su les dompter, et les ruines ...

  17. A real-time PCR diagnostic method for detection of Naegleria fowleri.

    Science.gov (United States)

    Madarová, Lucia; Trnková, Katarína; Feiková, Sona; Klement, Cyril; Obernauerová, Margita

    2010-09-01

    Naegleria fowleri is a free-living amoeba that can cause primary amoebic meningoencephalitis (PAM). While, traditional methods for diagnosing PAM still rely on culture, more current laboratory diagnoses exist based on conventional PCR methods; however, only a few real-time PCR processes have been described as yet. Here, we describe a real-time PCR-based diagnostic method using hybridization fluorescent labelled probes, with a LightCycler instrument and accompanying software (Roche), targeting the Naegleria fowleriMp2Cl5 gene sequence. Using this method, no cross reactivity with other tested epidemiologically relevant prokaryotic and eukaryotic organisms was found. The reaction detection limit was 1 copy of the Mp2Cl5 DNA sequence. This assay could become useful in the rapid laboratory diagnostic assessment of the presence or absence of Naegleria fowleri.

  18. Comparison of next-generation sequencing systems.

    Science.gov (United States)

    Liu, Lin; Li, Yinhu; Li, Siliang; Hu, Ni; He, Yimin; Pong, Ray; Lin, Danni; Lu, Lihua; Law, Maggie

    2012-01-01

    With fast development and wide applications of next-generation sequencing (NGS) technologies, genomic sequence information is within reach to aid the achievement of goals to decode life mysteries, make better crops, detect pathogens, and improve life qualities. NGS systems are typically represented by SOLiD/Ion Torrent PGM from Life Sciences, Genome Analyzer/HiSeq 2000/MiSeq from Illumina, and GS FLX Titanium/GS Junior from Roche. Beijing Genomics Institute (BGI), which possesses the world's biggest sequencing capacity, has multiple NGS systems including 137 HiSeq 2000, 27 SOLiD, one Ion Torrent PGM, one MiSeq, and one 454 sequencer. We have accumulated extensive experience in sample handling, sequencing, and bioinformatics analysis. In this paper, technologies of these systems are reviewed, and first-hand data from extensive experience is summarized and analyzed to discuss the advantages and specifics associated with each sequencing system. At last, applications of NGS are summarized.

  19. Ultraviolet, optical, infrared, and microwave observations of HR 5110

    Science.gov (United States)

    Little-Marenin, I. R.; Simon, T.; Ayres, T. R.; Cohen, N. L.; Feldman, P. A.

    1986-01-01

    Near-IR JHKLM photometric data and VLBI and IUE radio burst data collected on the short-period spectroscopic binary HR 5110 are analyzed to classify the object. The observed broadband colors were indicative of an F2 IV primary and a spotted K0 IV secondary. The system is being viewed pole-on, so is observable in the UV since the K companion has filled its Roche lobe. In comparisons of such features of Algol and RS VCn stars as the mass ratio, orbital inclination, presence or absence of evidence for mass streams, accretion disks, and active regions, spectral signatures, etc., sufficient similarities are found to classify HR 5110 as an Algol system.

  20. The rings of Uranus - Nature and origin

    Science.gov (United States)

    Dermott, S. F.; Gold, T.; Sinclair, A. T.

    1979-01-01

    Effects of a resonance between a set of ring particles and individual satellites are considered. It is suggested that the resonances are 1:1 for the rings of Uranus and that each ring must contain an as-yet unseen satellite. A model is proposed in which the rings of Uranus were caused by small satellites that entered the Roche zone due to tidal drag. According to this model, the satellites gradually lost material from their surfaces in this zone, the particles left a satellite with relatively low speeds and must have entered orbits similar to that of the satellite, and the gravitational force of the satellite compelled these particles to remain in a narrow sharply defined ring.

  1. Changes in the structure of the accretion disc of HS1804+67 through the outburst cycle

    CERN Document Server

    Baptista, R; Baptista, Raymundo

    1999-01-01

    We report on the analysis of high-speed photometry of the dwarf-nova HS1804+67 through its outburst cycle with eclipse mapping techniques. Eclipse maps show evidences of the formation of a spiral structure in the disc at the early stages of the outburst and reveal how the disc expands during the rise until its fills most of the primary Roche lobe at maximum light. During the decline phase, the disc becomes progressively fainter as the cooling front moves inwards from the outer regions, until only a small bright region around the white dwarf is left at minimum light. The variable part of the uneclipsed light is possibly due to emission in a wind emanating from the inner parts of the disc. The emission from this region is sensitive to the mass accretion rate.

  2. Period change investigation of the low mass ratio contact binary BO Ari

    Science.gov (United States)

    Kriwattanawong, W.; Tasuya, O.; Poojon, P.

    2016-04-01

    A photometric study and period change analysis for the A-type low mass ratio contact binary BO Ari is presented. The BVR light curves were fitted by using the Wilson-Devinney method. The photometric solution yields a low mass ratio of q = 0.1754(±0.0016) with a contact degree of f = 27.72%(±2.37%). We found a long-term orbital period decrease at a rate of dPdt = - 3.49 ×10-7 d yr-1. This result indicates that the system is undergoing mass transfer from the primary component to the secondary with a mass transfer rate of m˙1m1 = - 7.77 ×10-8 yr-1. With the period decrease, the inner and outer critical Roche surfaces will tighten and cause the degree of contact to increase. Therefore, BO Ari may evolve into a deeper contact system.

  3. Étude comparative multiéchelle des mécanismes de frottement et d’usure de carbures cémentés WC-Co

    OpenAIRE

    Yahiaoui, Malik; Paris, Jean-Yves; Denape, Jean; Dourfaye, Alfazazi

    2015-01-01

    Les carbures cémentés WC-Co sont très largement utilisés dans les industries de la coupe des métaux et de l’excavation des roches. De manière générale, le compromis dureté/ténacité remarquable de ces matériaux permet d’obtenir des outils à la fois résistants à l’usure et aux impacts. Chermant et Osterstock [1] ont été les premiers à expliciter des mécanismes entraînant l’usure des WC-Co. Ils ont ainsi expliqué que l’usure des WC-Co se produit principalement suite à des fissurations dans la ph...

  4. Établissement d’un critère de qualité des taillants diamant/WC-Co pour l’excavation de formations rocheuses

    OpenAIRE

    Yahiaoui, Malik; Gerbaud, Laurent; Paris, Jean-Yves; Delbé, Karl; Denape, Jean; Dourfaye, Alfazazi

    2012-01-01

    Sous des conditions géologiques extrêmes, les outils de forage composés d’inserts en diamant polycristallins (PDC) sont particulièrement intéressants au vu de leur performance de coupe [1]. La qualité des matériaux innovants utilisés pour la fabrication de ces outils PDC doit être déterminée avec précision par la mesure de leur efficacité de coupe et de leur résistance à l’usure. Un banc d’usure (tour) composé de roches formées par un mortier spécifique a été utilisé pour réaliser les expérie...

  5. Etude fondamentale de l'imbibition dans un réservoir fissuré Basic Research on Inbibition in a Fractured Reservoir

    Directory of Open Access Journals (Sweden)

    Iffly R.

    2006-11-01

    Full Text Available L'efficacité du balayage d'un gisement fissuré par de l'eau d'injection dépend étroitement de l'importance et de la vitesse d'imbibition de l'eau par les blocs matriciels. De nombreuses expériences de laboratoire, réalisées sur des échantillons de roche et avec les fluides provenant d'un gisement exploité par Elf, ont permis de préciser l'influence, sur la récupération de l'huile, de la hauteur et de la perméabilité des blocs, ainsi que des conditions régnant à leurs limites (certaines fissures peuvent contenir, soit de l'eau, soit de l'huile, soit encore être étanches. Les résultats mettent en évidence le rôle essentiel des liaisons physico-chimiques entre l'eau de gisement, l'eau injectée, l'huile et la roche, à tout moment de l'imbibition. De légers écarts dans la composition lithologique de la roche peuvent modifier considérablement les lois de récupération. Par ailleurs, la présence dans les fluides de certaines molécules organiques peut influencer la récupération de l'huile beaucoup plus que ce que l'on pourrait attendre a priori des seules variations du terme a cos 6,. Comme ces liaisons physico-chimiques sont encore mal connues, donc non modélisables, il n'est en général pas légitime de procéder à des expériences d'imbibition avec des fluides et/ou des échantillons de roche ne provenant pas du gisement étudié. Dans ces conditions, les résultats de cette étude sont nécessairement spécifiques du champ considéré. Toutefois, les méthodes utilisées, l'analyse des résultats qui est faite, ainsi que l'influence des principaux paramètres sur ces résultats, présentent un degré de généralité évident. Les mesures de laboratoire, complétées par des simulations numériques, ont conduit à un ensemble cohérent de résultats permettant de comprendre le rôle respectif de la gravité, de la capillarité, des conditions aux limites et des effets d'extrémité, entre autres. Waterflood

  6. International conference entitled Zdeněk Kopal’s Binary Star Legacy

    CERN Document Server

    Drechsel, Horst; ZDENEK KOPAL’S BINARY STAR LEGACY

    2005-01-01

    An international conference entitled "Zdenek Kopal's Binary Star Legacy" was held on the occasion of the late Professor Kopal's 90th birthday in his home town of Litomyšl/Czech Republic and dedicated to the memory of one of the leading astronomers of the 20th century. Professor Kopal, who devoted 60 years of his scientific life to the exploration of close binary systems, initiated a breakthrough in this field with his description of binary components as non-spherical stars deformed by gravity, with surfaces following Roche equipotentials. Such knowledge triggered the development of new branches of astrophysics dealing with the structure and evolution of close binaries and the interaction effects displayed by exciting objects such as cataclysmic variables, symbiotic stars or X-ray binaries. Contributions to this conference included praise of the achievements of a great astronomer and personal reminiscences brought forward by Kopal's former students and colleagues, and reflected the state of the art of the dyn...

  7. Modeling Multi-Wavelength Stellar Astrometry. I. SIM Lite Observations of Interacting Binaries

    CERN Document Server

    Coughlin, Jeffrey L; Harrison, Thomas E; Hoard, D W; Ciardi, David R; Benedict, G Fritz; Howell, Steve B; McArthur, Barbara E; Wachter, Stefanie

    2010-01-01

    Interacting binaries consist of a secondary star which fills or is very close to filling its Roche lobe, resulting in accretion onto the primary star, which is often, but not always, a compact object. In many cases, the primary star, secondary star, and the accretion disk can all be significant sources of luminosity. SIM Lite will only measure the photocenter of an astrometric target, and thus determining the true astrometric orbits of such systems will be difficult. We have modified the Eclipsing Light Curve code (Orosz & Hauschildt 2000) to allow us to model the flux-weighted reflex motions of interacting binaries, in a code we call REFLUX. This code gives us sufficient flexibility to investigate nearly every configuration of interacting binary. We find that SIM Lite will be able to determine astrometric orbits for all sufficiently bright interacting binaries where the primary or secondary star dominates the luminosity. For systems where there are multiple components that comprise the spectrum in the op...

  8. Effect of estimated glomerular filtration rate on plasma concentrations of B-type natriuretic peptides measured with multiple immunoassays in elderly individuals

    DEFF Research Database (Denmark)

    Schou, M.; Alehagen, U.; Goetze, J.P.;

    2009-01-01

    OBJETIVE: This study was designed to quantify the crude and adjusted effects of estimated glomerular filtration rate (eGFR) on N-terminal-pro-brain-natriuretic peptide (proBNP) measured with three immunoassays and brain natriuretic peptide (BNP) in elderly individuals. DESIGN: Cross-sectional study....... SETTING: 474 elderly outpatients with suspected heart failure (prevalence 13%) from the primary care. MAIN OUTCOME MEASURES: The effects of eGFR on proBNP, measured with three different immunoassays (Roche Diagnostics, Oslo and Copenhagen), and BNP (Shionogi) concentrations were evaluated by multiple...... linear regression models. RESULTS: In univariate analyses the effect of a 10% decrease in eGFR on proBNP concentrations was a 15% (95% confidence interval 11% to 18%), 9% (5% to 13%) and 21% (14% to 28%) increase. In multivariate models the effect was a 7% (3% to 11%), 4% (2% to 6%) and 13% (4% to 20...

  9. High Mass X-ray Binaries: Progenitors of double neutron star systems

    CERN Document Server

    Chaty, Sylvain

    2015-01-01

    In this review I briefly describe the nature of the three kinds of High-Mass X-ray Binaries (HMXBs), accreting through: (i) Be circumstellar disc, (ii) supergiant stellar wind, and (iii) Roche lobe filling supergiants. A previously unknown population of HMXBs hosting supergiant stars has been revealed in the last years, with multi-wavelength campaigns including high energy (INTEGRAL, Swift, XMM, Chandra) and optical/infrared (mainly ESO) observations. This population is divided between obscured supergiant HMXBs, and supergiant fast X-ray transients (SFXTs), characterized by short and intense X-ray flares. I discuss the characteristics of these types of supergiant HMXBs, propose a scenario describing the properties of these high-energy sources, and finally show how the observations can constrain the accretion models (e.g. clumpy winds, magneto-centrifugal barrier, transitory accretion disc, etc). Because they are the likely progenitors of Luminous Blue Variables (LBVs), and also of double neutron star systems,...

  10. Detection of EGFR mutations in plasma and biopsies from non-small cell lung cancer patients by allele-specific PCR assays

    DEFF Research Database (Denmark)

    Weber, Britta; Meldgaard, Peter; Hager, Henrik;

    2014-01-01

    BACKGROUND: Lung cancer patients with mutations in the epidermal growth factor receptor (EGFR) are primary candidates for EGFR-targeted therapy. Reliable analyses of such mutations have previously been possible only in tumour tissue. Here, we demonstrate that mutations can be detected in plasma...... of mutations in exons 18-21 of the EGFR gene, employing the cobas(®) EGFR Tissue Test and cobas(®) EGFR Blood Test (in development, Roche Molecular Systems, Inc., CA, USA). RESULTS: Test results were obtained in all 199 (100%) plasma samples and 196/199 (98%) of the biopsies. EGFR-activating mutations were...... identified in 24/199 (12%) plasma samples and 28/196 (14%) biopsy samples, and 17/196 (9%) matched pairs contained the same mutation. Six EGFR mutations were present only in plasma samples but not in the biopsy samples. The overall concordance of the EGFR gene mutations detected in plasma and biopsy tissue...

  11. Optical/infrared observations unveiling the formation, nature and evolution of High-Mass X-ray Binaries

    CERN Document Server

    Chaty, Sylvain

    2014-01-01

    In this review I first describe the nature of the three kinds of High-Mass X-ray Binaries (HMXBs), accreting through: (i) Be circumstellar disc, (ii) supergiant stellar wind, and (iii) Roche lobe filling supergiants. I then report on the discovery of two new populations of HMXBs hosting supergiant stars, recently revealed by a wealth of new observations, coming from the high energy side (INTEGRAL, Swift, XMM, Chandra satellites), and complemented by multi-wavelength optical/infrared observations (mainly ESO facilities). The first population is constituted of obscured supergiant HMXBs, the second one of supergiant fast X-ray transients (SFXTs), exhibiting short and intense X-ray flares. I finally discuss the formation and evolution of HMXBs, constrain the accretion models (e.g. clumpy winds, transitory accretion disc, magneto-centrifugal barrier), show evidences suggesting the existence of an evolutionary link, include comparisons with population synthesis models, and finally build a consistent scenario explai...

  12. Doppler Tomography in Cataclysmic Variables: an historical perspective

    CERN Document Server

    Echevarria, J

    2012-01-01

    To mark the half-century anniversary of this newly-born field of Cataclysmic Variables, a special emphasis is made in this review, on the Doppler Effect as a tool in astrophysics. The Doppler Effect was in fact, discovered almost 170 years ago, and has been since, one of the most important tools which helped to develop modern astrophysics. We describe and discuss here, its use in Cataclysmic Variables which, combined with another important tool, the tomography, first devised for medical purposes 70 years ago, helped to devise the astronomical Doppler Tomography, developed only two decades ago. A discussion is made since the first trailed spectra provided a one dimensional analysis of these binaries; on the establishment of a 2D velocity profiling of the accretion discs; and unto modern techniques, which include Roche Tomography, time modulation and 3D imaging.

  13. Editorial

    Directory of Open Access Journals (Sweden)

    Henry Bakis

    2015-02-01

    Full Text Available Le présent numéro de Netcom présente quelques articles sur le thème : Géopolitique de l’espace cybernétique en commençant par une exploration proposée par Henry Bakis de la fragilité de l’espace cybernétique et de l’urgence d’une sécurisation d’applications indispensables à la vie économique actuelle. Alors que l’espace cybernétique est devenu une incontournable dimension des politiques de défense et sécurité, Edward M. Roche et Michael J. Blaine lancent un appel, peut-être historique, pour u...

  14. Supercuspidal characters of $\\operatorname{SL}_2$ over a $p$-adic field

    CERN Document Server

    Adler, Jeffrey D; Sally, Paul J; Jr.,; Spice, Loren

    2010-01-01

    The character formulas of Sally and Shalika are an early triumph in $p$-adic harmonic analysis, but, to date, the calculations underlying the formulas have not been available. In this paper, which should be viewed as a precursor of the forthcoming volume by the authors and Alan Roche, we leverage modern technology (for example, the Moy-Prasad theory) to compute explicit character tables. An interesting highlight is the computation of the 'exceptional' supercuspidal characters, i.e., those depth-zero representations not arising by inflation-induction from a Deligne-Lusztig representation of finite $\\operatorname{SL}_2$; this provides a concrete application for the recent work of DeBacker and Kazhdan.

  15. The Tamiflu saga continues: will our conduct change after the publication of the latest systematic review on benefits and harms of oseltamivir?

    Science.gov (United States)

    Bachelet, Vivienne C

    2014-05-20

    In 2013, we wrote about the harm, waste and deception stemming from conducts adopted by the pharmaceutical industry, by concealing raw data and Clinical Study Reports (CSRs) from the regulator’s view when requesting the marketing patent. We described the case of Tamiflu (Roche), a drug that has been widely used in our population and profusely prescribed by physicians. Health authorities, entailing a great cost for the countries in the region, have also purchased it. In this editorial, we will show how the idea of using antivirals for prophylaxis and treatment of influenza took hold, starting from the first enthusiastic recommendations up to the systematic review published last month in the BMJ.

  16. The complete mitochondrial genome of the sandbar shark Carcharhinus plumbeus.

    Science.gov (United States)

    Blower, Dean C; Ovenden, Jennifer R

    2016-01-01

    The sandbar shark, Carcharhinus plumbeus, a major representative species in shark fisheries worldwide is now considered vulnerable to overfishing. A pool of 774,234 Roche 454 shotgun sequences from one individual were assembled into a 16,706 bp mitogenome with 33× average coverage depth. It comprised 13 protein coding genes, 22 transfer RNA's, 2 ribosomal genes and 2 non-coding regions, typical of a vertebrate mitogenome. As expected for sharks, an A-T nucleotide bias was evident. This adds to rapidly growing number of mitogenome assemblies for the economically important Carcharhinidae family. The C. plumbeus mitogenome will assist researchers, fisheries and conservation managers interested in shark molecular systematics, phylogeography, conservation genetics, population and stock structure. PMID:24938089

  17. Asymptotic cyclic cohomology

    CERN Document Server

    Puschnigg, Michael

    1996-01-01

    The aim of cyclic cohomology theories is the approximation of K-theory by cohomology theories defined by natural chain complexes. The basic example is the approximation of topological K-theory by de Rham cohomology via the classical Chern character. A cyclic cohomology theory for operator algebras is developed in the book, based on Connes' work on noncommutative geometry. Asymptotic cyclic cohomology faithfully reflects the basic properties and features of operator K-theory. It thus becomes a natural target for a Chern character. The central result of the book is a general Grothendieck-Riemann-Roch theorem in noncommutative geometry with values in asymptotic cyclic homology. Besides this, the book contains numerous examples and calculations of asymptotic cyclic cohomology groups.

  18. CLINICAL PERFORMANCE CHARACTERISTICS OF ELECSYS® FREE-ΒHCG AND PAPP-A FOR FIRST TRIMESTER TRISOMY 21 RISK ASSESSMENT IN GESTATIONAL WEEKS 8+0 TO 14+0

    DEFF Research Database (Denmark)

    Tørring, Niels; aulesa, C; Eiben, Bernd;

    2014-01-01

    Background Screening for fetal trisomy 21 (T21) in the first trimester includes analysis of the serological markers pregnancy-associated plasma protein A (PAPP-A) and free beta choriogonadotropin (free βhCG). With the launch of these assays on the cobas e and Elecsys platforms, we investigated...... their clinical and analytical performance. Patients and Methods We conducted a multicenter study in 5397 pregnancies including 108 cases with verified fetal T21 at 8 to 14 weeks of gestation. A technical validation of the Roche Elecsys® free βhCG and PAPP-A assays were performed, including method comparisons...... with the Brahms Kryptor®, PerkinElmer AutoDELFIA® and Siemens IMMULITE® assays. Furthermore a clinical validation including generation of assay specific medians from gestational age 8+0 to 14+0 weeks, and clinical test performance of risk assessment was performed. Results The imprecision of the Elecsys free βh...

  19. Fragilité du géocyberespace à l’heure des conflits cybernétiques

    Directory of Open Access Journals (Sweden)

    Henry Bakis

    2015-02-01

    Full Text Available Alors que les actes d’hostilité cybernétique se multiplient à travers le monde et que la course aux armements cybernétiques s’accélère. Cela met en lumière la vulnérabilité des TIC à toutes les échelles. Malgré l’importance du problème posé, le contrôle efficace de la sécurité de l’Internet soulève de grandes difficultés (politiques, législatives et techniques. Cette note est une introduction à l’article suivant dû à E. M. Roche & M. J. Blaine, article qui se conclut sur un appel à la signature d’une Convention internationale de désarmement cybernétique.

  20. Real-time quantitative PCR of microdissected paraffin-embedded breast carcinoma

    DEFF Research Database (Denmark)

    Gjerdrum, Lise Mette; Sorensen, Boe Sandahl; Kjeldsen, Eigil;

    2004-01-01

    We studied the feasibility of using real-time quantitative PCR to determine HER-2 DNA amplification and mRNA expression in microdissected formalin-fixed, paraffin-embedded breast tumors and compared this with standard immunohistochemistry (IHC) and fluorescent in situ hybridization (FISH) methods....... A single DCIS case was amplified in FISH, but not in IHC. Both HER-2 gene amplification and expression could be quantified in microdissected paraffin-embedded tumors using real-time PCR, DNA and RNA being successfully detected in 146 of 150 (97%) and 141 of 150 (94%) samples, respectively. PCR analysis...... for HER-2 DNA amplification using the LightCycler HER2/neu DNA Quantification kit (Roche Molecular Biochemicals, Mannheim, Germany) correlated fairly well with IHC and FISH. All IHC HER-2 3+ tumors were amplified according to the kit, as was the FISH-amplified DCIS case. DNA-PCR identified five additional...