WorldWideScience

Sample records for bovine nuclear transfer

  1. Nucleolar ultrastructure in bovine nuclear transfer embryos

    DEFF Research Database (Denmark)

    Kaňka, Jiří; Smith, Steven Dale; Soloy, Eva;

    1999-01-01

    in nuclear morphology as a transformation of the nucleolus precursor body into a functional rRNA synthesising nucleolus with a characteristic ultrastructure. We examined nucleolar ultrastructure in bovine in vitro produced (control) embryos and in nuclear transfer embryos reconstructed from a MII phase...... at 1 hr after fusion and, by 3 hr after fusion, it was restored again. At this time, the reticulated fibrillo-granular nucleolus had an almost round shape. The nucleolar precursor body seen in the two-cell stage nuclear transfer embryos consisted of intermingled filamentous components and secondary...... time intervals after fusion. In the two-cell stage nuclear transfer embryo, the originally reticulated nucleolus of the donor blastomere had changed into a typical nucleolar precursor body consisting of a homogeneous fibrillar structure. A primary vacuole appeared in the four-cell stage nuclear...

  2. Generation of bovine transgenics using somatic cell nuclear transfer

    Directory of Open Access Journals (Sweden)

    Stice Steven L

    2003-11-01

    Full Text Available Abstract The ability to produce transgenic animals through the introduction of exogenous DNA has existed for many years. However, past methods available to generate transgenic animals, such as pronuclear microinjection or the use of embryonic stem cells, have either been inefficient or not available in all animals, bovine included. More recently somatic cell nuclear transfer has provided a method to create transgenic animals that overcomes many deficiencies present in other methods. This review summarizes the benefits of using somatic cell nuclear transfer to create bovine transgenics as well as the possible opportunities this method creates for the future.

  3. Nuclear and nuclear reprogramming during the first cell cycle in bovine nuclear transfer embryos

    DEFF Research Database (Denmark)

    Østrup, Olga; Petrovicova, Ida; Strejcek, Frantisek;

    2009-01-01

    , somatic cell nuclei introduced into enucleated oocytes displayed chromatin condensation, partial nuclear envelope breakdown, nucleolar desegregation and transcriptional quiescence already at 0.5 hpa. Somatic cell cytoplasm remained temporally attached to introduced nucleus and nucleolus was partially...

  4. Effective Oocyte Vitrification and Survival Techniques for Bovine Somatic Cell Nuclear Transfer.

    Science.gov (United States)

    Park, Min Jee; Lee, Seung Eun; Kim, Eun Young; Lee, Jun Beom; Jeong, Chang Jin; Park, Se Pill

    2015-06-01

    Bovine somatic cell nuclear transfer (SCNT) using vitrified-thawed (VT) oocytes has been studied; however, the cloning efficiency of these oocytes is not comparable with that of nonvitrified (non-V) fresh oocytes. This study sought to optimize the survival and cryopreservation of VT oocytes for SCNT. Co-culture with feeder cells that had been preincubated for 15 h significantly improved the survival of VT oocytes and their in vitro developmental potential following SCNT in comparison to co-culture with feeder cells that had been preincubated for 2, 5, or 24 h (pEVT) group, 13.7%; VT group, 15.0%; p<0.05] and was comparable with that of the non-V group (25.9%). The reactive oxygen species level was significantly lower in the EAVT group than in the other vitrification groups (p<0.05). mRNA levels of maternal genes (ZAR1, BMP15, and NLRP5) and a stress gene (HSF1) were lower in the vitrification groups than in the non-V group (p<0.05), whereas the level of phospho-p44/42 mitogen-activated protein kinase did not differ among the groups. Among the vitrification groups, blastocysts in the EAVT group had the best developmental potential, as judged by their high mRNA expression of developmental potential-related genes (POU5f1, Interferon-tau, and SLC2A5) and their low expression of proapoptotic (CASP3) and stress (Hsp70) genes. This study demonstrates that SCNT using bovine frozen-thawed oocytes can be successfully achieved using optimized vitrification and co-culture techniques. PMID:25984830

  5. Gestaciones producidas con embriones bovinos clonados por transferencia nuclear Pregnancies produced by bovine embryos cloned by nuclear transfer

    Directory of Open Access Journals (Sweden)

    M A Martínez Díaz

    2007-01-01

    Full Text Available El presente estudio comunica la obtención de gestaciones de embriones clonados por transferencia nuclear de células somáticas en bovinos por primera vez en Chile. Ovocitos bovinos obtenidos de ovarios de matadero fueron madurados in vitro y enucleados por micromanipulación. Células donantes de núcleos fueron obtenidas de la oreja de una vaca adulta, cultivadas por 9-14 días y criopreservadas en nitrógeno líquido. Células somáticas confluentes fueron desagregadas e insertadas individualmente en el espacio perivitelino de un ovocito enucleado. Cada par ovocito-célula obtenido fue tratado con dos pulsos eléctricos para inducir su fusión y luego los embriones fueron cultivados por 2 horas y tratados con ionomicina y 6-dimetilaminopurina para su activación. Los embriones fueron cultivados en medio sintético oviductual por 8-9 días hasta el estado de blastocisto. Blastocistos fueron transferidos a vaquillas receptoras 7 a 9 días postcelo. Se realizaron 25 transferencias a vaquillas receptoras y se logró la preñez en 5 (20% de ellas. Dos de éstas abortaron a los 42 días y una tercera a los 120 días. Las dos vaquillas preñadas restantes mantuvieron su gestación (más de 45 días hasta el momento de escribir esta comunicación.With the aim of obtaining pregnancies from nuclear transfer embryos reconstituted with somatic cells and enucleated oocytes, bovine oocytes from slaughterhouse were matured in vitro and enucleated by micromanipulation. Nuclear donor cells were obtained from the ear of an adult cow, cultured for 9 to 14 days and cryopreserved in liquid nitrogen. Confluent cells were inserted individually in the perivitelline space of enucleated oocytes and treated with 2 electric pulses to induce fusion. The reconstituted zygotes were then cultured for 2 hours and treated with ionomycin and 6-dimethylaminopurine for their activation. The embryos were cultured in synthetic oviduct fluid for 8 to 9 days to obtain the

  6. Simplification of bovine somatic cell nuclear transfer by application of a zona-free manipulation technique

    DEFF Research Database (Denmark)

    Booth, P J; Tan, S J; Reipurth, R;

    2001-01-01

    Contemporary nuclear transfer techniques often require the involvement of skilled personnel and extended periods of micromanipulation. Here, we present details of the development of a nuclear transfer technique for somatic cells that is both simpler and faster than traditional methods......, and (c) establish any potential embryotoxic effects of PHA-P. The initial data indicated that, of calcium ionophore A23187, ionomycin, and electropulse treatments as primary activation agents, the two former were equally efficient even with reduced exposure times. WOW-culture of zona-free versus zona.......8% of cultured oocytes). Subsequent application of the optimized technique for nuclear transfer using nine different granulosa cell primary cultures (cultured in 0.5% serum for 5-12 days) generated 37.6 +/- 3.9% (11 replicates; range, 16.4-58.1 blastocysts per successfully fused and surviving reconstructed...

  7. DNA methylation status of H19 and Xist genos in lungs of somatic cell nuclear transfer bovines

    Institute of Scientific and Technical Information of China (English)

    CHEN Jie; LI DongJie; LIU YanQin; ZHANG Cui; DAI YunPing; LI ShiJie; LINing

    2008-01-01

    In somatic cell nuclear transfer (SCNT) technologies, the donor cell's nuclei need to be epigenetically reprogrammed for embryonic development. The incomplete reprogramming of donor cell nuclei has been implicated as a primary reason for the low efficiency of SCNT. DNA methylation is a major epige- netic modification of the genome that regulates crucial aspects of genome function, including estab-lishment of genomic imprinting. In order to make sure whether the DNA methylation reprogramming is efficient in SCNT animals, we analyzed the DNA methylation status of two imprinting genes, H19 and Xist, in lungs of deceased SCNT bovines that died within 48 h of birth using bisulfite sequencing analysis. Our findings demonstrated that cloned bovines showed significantly lower DNA methylation of H19 than controls (P<0.05), and three tested CpGs sites (1, 2, 3) exhibited unmethylation in one cloned bovine (9C3); however, Xist showed similar DNA methylation levels between clones and con- trols, and both showed hypermethylation (96.11% and 86.67%).

  8. Cloned embryos from semen. Part 2: Intergeneric nuclear transfer of semen-derived eland (Taurotragus oryx) epithelial cells into bovine oocytes

    Science.gov (United States)

    Nel-Themaat, L.; Gomez, M.C.; Pope, C.E.; Lopez, M.; Wirtu, G.; Jenkins, J.A.; Cole, A.; Dresser, B.L.; Bondioli, K.R.; Godke, R.A.

    2008-01-01

    The production of cloned offspring by nuclear transfer (NT) of semen-derived somatic cells holds considerable potential for the incorporation of novel genes into endangered species populations. Because oocytes from endangered species are scarce, domestic species oocytes are often used as cytoplasts for interspecies NT. In the present study, epithelial cells isolated from eland semen were used for intergeneric transfer (IgNT) into enucleated bovine oocytes and compared with bovine NT embryos. Cleavage rates of bovine NT and eland IgNT embryos were similar (80 vs. 83%, respectively; p > 0.05); however, development to the morula and blastocyst stage was higher for bovine NT embryos (38 and 21%, respectively; p bovine NT or eland IgNT cybrids before activation, but in 75 and 70% of bovine NT and eland igNT embryos, respectively, cell-cycle resumption was observed at 16 h postactivation (hpa). For eland IgNT embryos, 13% had ???8 cells at 84 hpa, while 32% of the bovine NT embryos had ???8 cells at the same interval. However, 100 and 66% of bovine NT and eland IgNT embryos, respectively, that had ???8 cells synthesized DNA. From these results we concluded that (1) semen-derived epithelial cell nuclei can interact and be transcriptionally controlled by bovine cytoplast, (2) the first cell-cycle occurred in IgNT embryos, (3) a high frequency of developmental arrest occurs before the eight-cell stage in IgNT embryos, and (4) IgNT embryos that progress through the early cleavage stage arrest can (a) synthesize DNA, (b) progress through subsequent cell cycles, and (c) may have the potential to develop further. ?? 2008 Mary Ann Liebert, Inc.

  9. Numerical Chromosome Errors in Day 7 Somatic Nuclear Transfer Bovine Blastocysts

    DEFF Research Database (Denmark)

    Booth, Paul J.; VIUFF, Dorte; Tan, Shijian;

    2002-01-01

    amino acids, myoinositol, sodium citrate, and 5% cattle serum in microwells for 7 days, at which time nuclei from all blastocysts were extracted and chromosome aberrations were evaluated using dual-color fluorescent in situ hybridization with bovine chromosome 6- and 7-specific probes. Five embryo clone...

  10. Embryo cloning by nuclear transfer in the bovine species: first results

    OpenAIRE

    Ectors, Francis; Ectors, Fabien; Delval, Alain; Thonon, Fabienne; Beckers, Jean-François

    1993-01-01

    Le clonage par transfert de noyau fut réalisé pour la première fois chez les bovins par Prather et collaborateurs en 1987. Vu l'importance économique de ce mode de multiplication, de nombreuses équipes de recherche affinent la technique et étudient sa mise en application sur le terrain. Alors que l'énucléation de l'ovocyte receveur, l'injection et la fusion du blastomère donneur se réalisent avec succès, la maturation ovocytaire, la culture et la congélation de l'embryon reconstitué posent en...

  11. Effect of two activation treatments and age of blastomere karyoplasts on in vitro development of bovine nuclear transfer embryos

    DEFF Research Database (Denmark)

    Booth, P J; Holm, P; Vajta, G;

    2001-01-01

    , or 5 in vitro produced donor embryos, were examined in order to define an optimal nuclear transfer protocol. The two activation protocols comprised calcium ionophore followed by either CHX or DMAP. Parthenogenetic blastocyst yields were greater (P

  12. Lowering storage temperature during ovary transport is beneficial to the developmental competence of bovine oocytes used for somatic cell nuclear transfer.

    Science.gov (United States)

    Wang, Y S; Zhao, X; Su, J M; An, Z X; Xiong, X R; Wang, L J; Liu, J; Quan, F S; Hua, S; Zhang, Y

    2011-03-01

    The objective of this study was to determine the effect of storage temperature during ovary transport on the developmental competence of bovine oocytes for use in somatic cell nuclear transfer (SCNT). Ovaries obtained from a slaughterhouse were stored in physiological saline for 3-4h at one of the three temperatures: 15 °C, 25 °C, or 35 °C. The developmental competence of oocytes used for SCNT was ascertained by cleavage and blastocyst formation rate, total cell number, apoptosis index, and the relative abundance of Bax and Hsp70.1 in day 7 blastocysts. Ovaries stored at 35 °C for 3-4h reduced the recovery rate of grade I and II oocytes compared with those stored at 25 °C or 15 °C (45.1±0.7% vs. 76.7±1.2% or 74.8±2.0%, Povaries stored at 15 °C, however, produced blastocysts with higher cell numbers (97.3±8.6 vs. 80.2±10.8 or 77.4±11.7; Povaries stored at 15 °C was lower than those stored at 25 °C or 35 °C (Pquality and developmental competence of oocytes used for SCNT due to the alleviation of stresses on the oocytes compared with those subjected to storage temperatures of 25 °C or 35 °C. PMID:21333472

  13. Expression Profile of Genes as Indicators of Developmental Competence and Quality of In Vitro Fertilization and Somatic Cell Nuclear Transfer Bovine Embryos

    Science.gov (United States)

    Monteleone, Melisa Carolina; Mucci, Nicolas; Kaiser, German Gustavo; Brocco, Marcela; Mutto, Adrián

    2014-01-01

    Reproductive biotechnologies such as in vitro fertilization (IVF) and somatic cell nuclear transfer (SCNT) enable improved reproductive efficiency of animals. However, the birth rate of in vitro-derived embryos still lags behind that of their in vivo counterparts. Thus, it is critical to develop an accurate evaluation and prediction system of embryo competence, both for commercial purposes and for scientific research. Previous works have demonstrated that in vitro culture systems induce alterations in the relative abundance (RA) of diverse transcripts and thus compromise embryo quality. The aim of this work was to analyze the RA of a set of genes involved in cellular stress (heat shock protein 70-kDa, HSP70), endoplasmic reticulum (ER) stress (immunoglobulin heavy chain binding protein, Bip; proteasome subunit β5, PSMB5) and apoptosis (BCL-2 associated X protein, Bax; cysteine aspartate protease-3, Caspase-3) in bovine blastocysts produced by IVF or SCNT and compare it with that of their in vivo counterparts. Poly (A) + mRNA was isolated from three pools of 10 blastocysts per treatment and analyzed by real-time RT-PCR. The RA of three of the stress indicators analyzed (Bax, PSMB5 and Bip) was significantly increased in SCNT embryos as compared with that of in vivo-derived blastocysts. No significant differences were found in the RA of HSP70 and Caspase-3 gene transcripts. This study could potentially complement morphological analyses in the development of an effective and accurate technique for the diagnosis of embryo quality, ultimately aiding to improve the efficiency of assisted reproductive techniques (ART). PMID:25269019

  14. Expression profile of genes as indicators of developmental competence and quality of in vitro fertilization and somatic cell nuclear transfer bovine embryos.

    Directory of Open Access Journals (Sweden)

    Maria Jesús Cánepa

    Full Text Available Reproductive biotechnologies such as in vitro fertilization (IVF and somatic cell nuclear transfer (SCNT enable improved reproductive efficiency of animals. However, the birth rate of in vitro-derived embryos still lags behind that of their in vivo counterparts. Thus, it is critical to develop an accurate evaluation and prediction system of embryo competence, both for commercial purposes and for scientific research. Previous works have demonstrated that in vitro culture systems induce alterations in the relative abundance (RA of diverse transcripts and thus compromise embryo quality. The aim of this work was to analyze the RA of a set of genes involved in cellular stress (heat shock protein 70-kDa, HSP70, endoplasmic reticulum (ER stress (immunoglobulin heavy chain binding protein, Bip; proteasome subunit β5, PSMB5 and apoptosis (BCL-2 associated X protein, Bax; cysteine aspartate protease-3, Caspase-3 in bovine blastocysts produced by IVF or SCNT and compare it with that of their in vivo counterparts. Poly (A + mRNA was isolated from three pools of 10 blastocysts per treatment and analyzed by real-time RT-PCR. The RA of three of the stress indicators analyzed (Bax, PSMB5 and Bip was significantly increased in SCNT embryos as compared with that of in vivo-derived blastocysts. No significant differences were found in the RA of HSP70 and Caspase-3 gene transcripts. This study could potentially complement morphological analyses in the development of an effective and accurate technique for the diagnosis of embryo quality, ultimately aiding to improve the efficiency of assisted reproductive techniques (ART.

  15. DNA methylation patterns in tissues from mid-gestation bovine foetuses produced by somatic cell nuclear transfer show subtle abnormalities in nuclear reprogramming

    Directory of Open Access Journals (Sweden)

    Lee Rita SF

    2010-03-01

    Full Text Available Abstract Background Cloning of cattle by somatic cell nuclear transfer (SCNT is associated with a high incidence of pregnancy failure characterized by abnormal placental and foetal development. These abnormalities are thought to be due, in part, to incomplete re-setting of the epigenetic state of DNA in the donor somatic cell nucleus to a state that is capable of driving embryonic and foetal development to completion. Here, we tested the hypothesis that DNA methylation patterns were not appropriately established during nuclear reprogramming following SCNT. A panel of imprinted, non-imprinted genes and satellite repeat sequences was examined in tissues collected from viable and failing mid-gestation SCNT foetuses and compared with similar tissues from gestation-matched normal foetuses generated by artificial insemination (AI. Results Most of the genomic regions examined in tissues from viable and failing SCNT foetuses had DNA methylation patterns similar to those in comparable tissues from AI controls. However, statistically significant differences were found between SCNT and AI at specific CpG sites in some regions of the genome, particularly those associated with SNRPN and KCNQ1OT1, which tended to be hypomethylated in SCNT tissues. There was a high degree of variation between individuals in methylation levels at almost every CpG site in these two regions, even in AI controls. In other genomic regions, methylation levels at specific CpG sites were tightly controlled with little variation between individuals. Only one site (HAND1 showed a tissue-specific pattern of DNA methylation. Overall, DNA methylation patterns in tissues of failing foetuses were similar to apparently viable SCNT foetuses, although there were individuals showing extreme deviant patterns. Conclusion These results show that SCNT foetuses that had developed to mid-gestation had largely undergone nuclear reprogramming and that the epigenetic signature at this stage was not a

  16. Nuclear transfer: Progress and quandaries

    OpenAIRE

    Zhou Qi; Jouneau Alice; Li Ziyi; Li Xuemei; Renard Jean-Paul

    2003-01-01

    Abstract Cloning mammals by nuclear transfer is a powerful technique that is quickly advancing the development of genetically defined animal models. However, the overall efficiency of nuclear transfer is still very low and several hurdles remain before the power of this technique will be fully harnessed. Among these hurdles include an incomplete understanding of biologic processes that control epigenetic reprogramming of the donor genome following nuclear transfer. Incomplete epigenetic repro...

  17. DNA methylation at a bovine alpha satellite I repeat CpG site during development following fertilization and somatic cell nuclear transfer.

    Directory of Open Access Journals (Sweden)

    Christine Couldrey

    Full Text Available Incomplete epigenetic reprogramming is postulated to contribute to the low developmental success following somatic cell nuclear transfer (SCNT. Here, we describe the epigenetic reprogramming of DNA methylation at an alpha satellite I CpG site (αsatI-5 during development of cattle generated either by artificial insemination (AI or in vitro fertilization (IVF and SCNT. Quantitative methylation analysis identified that SCNT donor cells were highly methylated at αsatI-5 and resulting SCNT blastocysts showed significantly more methylation than IVF blastocysts. At implantation, no difference in methylation was observed between SCNT and AI in trophoblast tissue at αsatI-5, however, SCNT embryos were significantly hyper-methylated compared to AI controls at this time point. Following implantation, DNA methylation at αsatI-5 decreased in AI but not SCNT placental tissues. In contrast to placenta, the proportion of methylation at αsatI-5 remained high in adrenal, kidney and muscle tissues during development. Differences in the average proportion of methylation were smaller in somatic tissues than placental tissues but, on average, SCNT somatic tissues were hyper-methylated at αsatI-5. Although sperm from all bulls was less methylated than somatic tissues at αsatI-5, on average this site remained hyper-methylated in sperm from cloned bulls compared with control bulls. This developmental time course confirms that epigenetic reprogramming does occur, at least to some extent, following SCNT. However, the elevated methylation levels observed in SCNT blastocysts and cellular derivatives implies that there is either insufficient time or abundance of appropriate reprogramming factors in oocytes to ensure complete reprogramming. Incomplete reprogramming at this CpG site may be a contributing factor to low SCNT success rates, but more likely represents the tip of the iceberg in terms of incompletely reprogramming. Until protocols ensure the epigenetic

  18. Transcriptional reprogramming of gene expression in bovine somatic cell chromatin transfer embryos

    OpenAIRE

    Page Grier P; Kasinathan Poothappillai; Wang Zhongde; Rodriguez-Osorio Nelida; Robl James M; Memili Erdogan

    2009-01-01

    Abstract Background Successful reprogramming of a somatic genome to produce a healthy clone by somatic cells nuclear transfer (SCNT) is a rare event and the mechanisms involved in this process are poorly defined. When serial or successive rounds of cloning are performed, blastocyst and full term development rates decline even further with the increasing rounds of cloning. Identifying the "cumulative errors" could reveal the epigenetic reprogramming blocks in animal cloning. Results Bovine clo...

  19. Transcriptional Reprogramming of Gene Expression in Bovine Somatic Cell Chromatin Transfer Embryos

    OpenAIRE

    Rodriguez-Osorio, N.; Wang, Zhongde; Page, G. P.; Robl, J M; Memili, E.

    2009-01-01

    Background Successful reprogramming of a somatic genome to produce a healthy clone by somatic cells nuclear transfer (SCNT) is a rare event and the mechanisms involved in this process are poorly defined. When serial or successive rounds of cloning are performed, blastocyst and full term development rates decline even further with the increasing rounds of cloning. Identifying the "cumulative errors" could reveal the epigenetic reprogramming blocks in animal cloning. Results Bovine clones from ...

  20. Transfer reactions in nuclear astrophysics

    Science.gov (United States)

    Bardayan, D. W.

    2016-08-01

    To a high degree many aspects of the large-scale behavior of objects in the Universe are governed by the underlying nuclear physics. In fact the shell structure of nuclear physics is directly imprinted into the chemical abundances of the elements. The tranquility of the night sky is a direct result of the relatively slow rate of nuclear reactions that control and determines a star’s fate. Understanding the nuclear structure and reaction rates between nuclei is vital to understanding our Universe. Nuclear-transfer reactions make accessible a wealth of knowledge from which we can extract much of the required nuclear physics information. A review of transfer reactions for nuclear astrophysics is presented with an emphasis on the experimental challenges and opportunities for future development.

  1. Cloning in cattle: from embryo splitting to somatic nuclear transfer.

    Science.gov (United States)

    Heyman, Y; Vignon, X; Chesné, P; Le Bourhis, D; Marchal, J; Renard, J P

    1998-01-01

    The ability to obtain genetically identical offspring in cattle (clones) is useful for research and for potential applications to breeding schemes. Experimental possibilities for generating such animals have evolved considerably in the last two decades. Embryo splitting has become a relatively simple technique but is limited to twinning. Embryonic nuclear transfer has improved and is associated with sexing to generate sets of clones despite a great variability of results between parent embryos. The factors of progress are reviewed here. Recently, somatic cells used as a source of nuclei in bovine nuclear transfer has been demonstrated. Here we present the results of the developmental potential of nuclei from skin and muscle cells.

  2. Dry spent nuclear fuel transfer

    International Nuclear Information System (INIS)

    Newport News Shipbuilding, (NNS), has been transferring spent nuclear fuel in a dry condition for over 25 years. It is because of this successful experience that NNS decided to venture into the design, construction and operation of a commercial dry fuel transfer project. NNS is developing a remote handling system for the dry transfer of spent nuclear fuel. The dry fuel transfer system is applicable to spent fuel pool-to-cask or cask-to-cask or both operations. It is designed to be compatible with existing storage cask technology as well as the developing multi-purpose canister design. The basis of NNS' design is simple. It must be capable of transferring all fuel designs, it must be capable of servicing 100 percent of the commercial nuclear plants, it must protect the public and nuclear operators, it must be operated cost efficiently and it must be transportable. Considering the basic design parameters, the following are more specific requirements included in the design: (a) Total weight of transfer cask less than 24 tons; (b) no requirement for permanent site modifications to support system utilization; (c) minimal radiation dose to operating personnel; (d) minimal generation of radioactive waste; (e) adaptability to any size and length fuel or cask; (f) portability of system allowing its efficient movement from site to site; (g) safe system; all possible ''off normal'' situations are being considered, and resultant safety systems are being engineered into NNS' design to mitigate problems. The primary focus of this presentation is to provide an overview of NNS' Dry Spent Nuclear Fuel Transfer System. (author). 5 refs

  3. Efeito do número da passagem e do gênero das células doadoras de núcleo no desenvolvimento de bovinos produzidos por transferência nuclear Effect of culture time and gender of nuclei donor cells on bovine development produced by nuclear transfer

    Directory of Open Access Journals (Sweden)

    Giovana Krempel Fonseca Merighe

    2010-10-01

    Full Text Available Objetivou-se com este trabalho avaliar o efeito do número da passagem e do sexo das células doadoras de núcleo no desenvolvimento embrionário e fetal após transferência nuclear. Para isso, oócitos bovinos foram maturados, enucleados e reconstruídos com células somáticas de animal adulto. Após a fusão e ativação química, os zigotos reconstituídos foram cultivados em Charles Rosenkranz 2 (CR2 com monocamada de células da granulosa a 38,8ºC em atmosfera umidificada a 5% de CO2 em ar, durante sete dias, e transferidos para receptoras sincronizadas. As taxas de clivagem e desenvolvimento a blastocisto de embriões reconstruídos com células cultivadas por tempo maior foram inferiores às obtidas com os demais tempos de cultivo. Além disso, os blastocistos produzidos não resultaram no desenvolvimento de uma gestação a termo. Embora a taxa de clivagem em embriões fêmeas tenha sido maior, o número de embriões que atingiram o estádio de blastocisto foi maior nos embriões machos. No período gestacional, fêmeas apresentaram maior taxa de aborto entre 90 e 120 dias de gestação. Esses resultados indicam que células doadoras de núcleos cultivados por longos períodos dificultam a produção de blastocistos e aumentam as chances de perdas durante a gestação. Embriões clonados machos têm maior competência para se desenvolver a blastocisto e resultam em menor taxa de perda gestacional.The objective of this study was to evaluate the effects of culture time and sex of nuclei donor cells on embryo and fetal development after nuclear transfer. Thus, bovine oocytes were matured, enucleated and reconstructed with somatic cells from an adult animal. After fusion and chemical activation, the reconstituted zygotes were cultured in Charles Rosenkranz 2 (CR2 on a granular monolayer cell at 38.8ºC in a humidified atmosphere 5% CO2 in air for seven days, and transferred to synchronized receptors. Cleavage rates and development to

  4. Transcriptional reprogramming of gene expression in bovine somatic cell chromatin transfer embryos

    Directory of Open Access Journals (Sweden)

    Page Grier P

    2009-04-01

    Full Text Available Abstract Background Successful reprogramming of a somatic genome to produce a healthy clone by somatic cells nuclear transfer (SCNT is a rare event and the mechanisms involved in this process are poorly defined. When serial or successive rounds of cloning are performed, blastocyst and full term development rates decline even further with the increasing rounds of cloning. Identifying the "cumulative errors" could reveal the epigenetic reprogramming blocks in animal cloning. Results Bovine clones from up to four generations of successive cloning were produced by chromatin transfer (CT. Using Affymetrix bovine microarrays we determined that the transcriptomes of blastocysts derived from the first and the fourth rounds of cloning (CT1 and CT4 respectively have undergone an extensive reprogramming and were more similar to blastocysts derived from in vitro fertilization (IVF than to the donor cells used for the first and the fourth rounds of chromatin transfer (DC1 and DC4 respectively. However a set of transcripts in the cloned embryos showed a misregulated pattern when compared to IVF embryos. Among the genes consistently upregulated in both CT groups compared to the IVF embryos were genes involved in regulation of cytoskeleton and cell shape. Among the genes consistently upregulated in IVF embryos compared to both CT groups were genes involved in chromatin remodelling and stress coping. Conclusion The present study provides a data set that could contribute in our understanding of epigenetic errors in somatic cell chromatin transfer. Identifying "cumulative errors" after serial cloning could reveal some of the epigenetic reprogramming blocks shedding light on the reprogramming process, important for both basic and applied research.

  5. Conjugative transfer of resistance determinants among human and bovine Streptococcus agalactiae

    OpenAIRE

    Tatiana Castro Abreu Pinto; Natália Silva Costa; Ana Beatriz de Almeida Corrêa; Ivi Cristina Menezes Oliveira; Marcos Correa de Mattos; Alexandre Soares Rosado; Leslie Claude Benchetrit

    2014-01-01

    Streptococcus agalactiae (GBS) is a major source of human perinatal diseases and bovine mastitis. Erythromycin (Ery) and tetracycline (Tet) are usually employed for preventing human and bovine infections although resistance to such agents has become common among GBS strains. Ery and Tet resistance genes are usually carried by conjugative transposons (CTns) belonging to the Tn916 family, but their presence and transferability among GBS strains have not been totally explored. Here we evaluated ...

  6. Epigenetic reprogramming in mammalian nuclear transfer

    Institute of Scientific and Technical Information of China (English)

    LI Shijie; DU Weihua; LI Ning

    2004-01-01

    Somatic cloning has been succeeded in some species, but the cloning efficiency is very low, which limits the application of the technique in many areas of research and biotechnology. The cloning of mammals by somatic cell nuclear transfer (NT) requires epigenetic reprogramming of the differentiated state of donor cell to a totipotent, embryonic ground state. Accumulating evidence indicates that incomplete or inappropriate epigenetic reprogramming of donor nuclei is likely to be the primary cause of failures in nuclear transfer. This review summarizes the roles of various epigenetic mechanisms, including DNA methylation, histone acetylation, imprinting, X-chromosome inactivation, telomere maintenance and expressions of development-related genes on somatic nuclear transfer.

  7. Canadian Experience in Nuclear Power Technology Transfer

    International Nuclear Information System (INIS)

    Technology transfer has and will continue to play a major role in the development of nuclear power programs. From the early beginnings of the development of the peaceful uses of nuclear power by just a few nations in the mid-1940s there has been a considerable transfer of technology and today 34 countries have nuclear programs in various stages of development. Indeed, some of the major nuclear vendors achieves their present position through a process of technology transfer and subsequent development. Canada, one of the early leaders in the development of nuclear power, has experience with a wide range of programs bout within its own borders and with other countries. This paper briefly describes this experience and the lessons learned from Canada's involvement in the transfer of nuclear power technology. Nuclear technology is complex and diverse and yet it can be assimilated by a nation given a fire commitment of both suppliers and recipients of technology to achieve success. Canada has reaped large benefits from its nuclear program and we believe this has been instrumentally linked to the sharing of goals and opportunity for participation over extended periods of time by many interests within the Canadian infrastructure. While Canada has accumulated considerable expertise in nuclear technology transfer, we believe there is still much for US to learn. Achieving proficiency in any of the many kinds of nuclear related technologies will place a heavy burden on the financial and human resources of a nation. Care must be taken to plan carefully the total criteria which will assure national benefits in industrial and economic development. Above all, effective transfer of nuclear technology requires a long term commitment by both parties

  8. Technology transfer from nuclear research

    International Nuclear Information System (INIS)

    A number of processes, components and instruments developed at the Bhabha Atomic Research Centre, (BARC), Bombay, find application in industry and are available for transfer to private or public sector undertakings for commercial exploitation. The Technology Transfer Group (TTG) constituted in January 1980 identifies such processes and prototypes which can be made available for transfer. This catalogue contains brief descriptions of such technologies and they are arranged under three groups, namely, Group A containing descriptions of technologies already transferred, Group B containing descriptions of technologies ready for transfer and Group C containing descriptions of technology transfer proposals being processed. The position in the above-mentioned groups is as on 1 March 1989. The BARC has also set up a Technology Corner where laboratory models and prototypes of instruments, equipment and components are displayed. These are described in the second part of the catalogue. (M.G.B.)

  9. Dry Transfer Systems for Used Nuclear Fuel

    Energy Technology Data Exchange (ETDEWEB)

    Brett W. Carlsen; Michaele BradyRaap

    2012-05-01

    The potential need for a dry transfer system (DTS) to enable retrieval of used nuclear fuel (UNF) for inspection or repackaging will increase as the duration and quantity of fuel in dry storage increases. This report explores the uses for a DTS, identifies associated general functional requirements, and reviews existing and proposed systems that currently perform dry fuel transfers. The focus of this paper is on the need for a DTS to enable transfer of bare fuel assemblies. Dry transfer systems for UNF canisters are currently available and in use for transferring loaded canisters between the drying station and storage and transportation casks.

  10. Cloned calves produced by nuclear transfer from cultured cumulus cells

    Institute of Scientific and Technical Information of China (English)

    AN; Xiaorong(安晓荣); GOU; Kemian(苟克勉); ZHU; Shien(朱士恩); GUAN; Hong(关宏); HOU; Jian(侯健); LIN; Aixing(林爱星); ZENG; Shenming(曾申明); TIAN; Jianhui(田见辉); CHEN; Yongfu(陈永福)

    2002-01-01

    Short-term cultured cumulus cell lines (1-5BCC) derived from 5 individual cows were used in nuclear transfer (NT) and 1188 enucleated bovine oocytes matured in vitro were used as nuclear recipients. A total of 931 (78.4%) cloned embryos were reconstructed, of which 763 (82%) cleaved, 627 (67.3%) developed to 8-cell stage, and 275 (29.5%) reached blastocyst stage. The average cell number of blastocysts was 124±24.5 (n=20). In this study, the effects of donor cell sources, serum starvation of donor cells, time interval from fusion to activation (IFA) were also tested on cloning efficiency. These results showed that blastocyst rates of embryos reconstructed from 5 different individuals cells were significantly different among them (14.1%, 45.2%, 27.3%, 34.3%, vs 1.5%, P0.05); and that blastocyst rate (20.3%) of the group with fusion/activation interval of 2-3 h, was significantly lower than that of the 3-6 h groups (31.0%), while not significantly different among 3-4 h (P < 0.05), 4-5 h, and 5-6 h groups (P ≥ 0.05). Sixty-three thawed NT blastocysts were transferred to 31 recipient cows, of which 4 pregnancies were established and two cloned calves were given birth. These results indicate that serum starvation of cumulus cells is not a key factor for successful bovine cloning, while IFA treatment and sources of donor cells have effects on cloning efficiency.

  11. Horizontal gene transfers link a human MRSA pathogen to contagious bovine mastitis bacteria.

    Directory of Open Access Journals (Sweden)

    Thomas Brody

    Full Text Available BACKGROUND: Acquisition of virulence factors and antibiotic resistance by many clinically important bacteria can be traced to horizontal gene transfer (HGT between related or evolutionarily distant microflora. Comparative genomic analysis has become an important tool for identifying HGT DNA in emerging pathogens. We have adapted the multi-genome alignment tool EvoPrinter to facilitate discovery of HGT DNA sequences within bacterial genomes and within their mobile genetic elements. PRINCIPAL FINDINGS: EvoPrinter analysis of 13 different Staphylococcus aureus genomes revealed that one of the human isolates, the hospital epidemic methicillin-resistant MRSA252 strain, uniquely shares multiple putative HGT DNA sequences with different causative agents of bovine mastitis that are not found in the other human S. aureus isolates. MRSA252 shares over 14 different DNA sequence blocks with the bovine mastitis ET3 S. aureus strain RF122, and many of the HGT DNAs encode virulence factors. EvoPrinter analysis of the MRSA252 chromosome also uncovered virulence-factor encoding HGT events with the genome of Listeria monocytogenes and a Staphylococcus saprophyticus associated plasmid. Both bacteria are also causal agents of contagious bovine mastitis. CONCLUSIONS: EvoPrinter analysis reveals that the human MRSA252 strain uniquely shares multiple DNA sequence blocks with different causative agents of bovine mastitis, suggesting that HGT events may be occurring between these pathogens. These findings have important implications with regard to animal husbandry practices that inadvertently enhance the contact of human and livestock bacterial pathogens.

  12. Sheep: The First Large Animal Model in Nuclear Transfer Research

    OpenAIRE

    Loi, Pasqualino; Czernik, Marta; Zacchini, Federica; Iuso, Domenico; Scapolo, Pier Augusto; Ptak, Grazyna

    2013-01-01

    The scope of this article is not to provide an exhaustive review of nuclear transfer research, because many authoritative reviews exist on the biological issues related to somatic and embryonic cell nuclear transfer. We shall instead provide an overview on the work done specifically on sheep and the value of this work on the greater nuclear transfer landscape.

  13. Production of transgenic calves by somatic cell nuclear transfer

    Institute of Scientific and Technical Information of China (English)

    GONG Guochun; WAN Rong; HUANG Yinghua; LI Ning; DAI Yunping; FAN Baoliang; ZHU Huabing; WANG Lili; WANG Haiping; TANG Bo; LIU Ying; LI Rong

    2004-01-01

    Bovine fetal oviduct epithelial cells were transfected with constructed double marker selective vector (pCE-EGFP-IRES-Neo-dNdB) containing the enhanced green fluorescent protein (EGFP) and neomycin-resistant (Neor) genes by electroporation, and a transgenic cell line was obtained. Somatic cell nuclear transfer (SCNT) was carried out using the transgenic cells as nuclei donor. A total of 424 SCNT embryos were reconstructed and 208 (49.1%) of them developed to blastocyst stage. 17 blastocysts on D 7 after reconstruction were transferred to 17 surrogate calves, and 5 (29.4%) recipients were found to be pregnant. Three of them maintained to term and delivered three cloned calves. PCR and Southern blot analysis confirmed the integration of transgene in all of the three cloned calves. In addition, expression of EGFP was detected in biopsy isolated from the transgenic cloned calves and fibroblasts derived from the biopsy. Our results suggest that transgenic calves could be efficiently produced by SCNT using transgenic cells as nuclei donor. Furthermore, all cloned animals could be ensured to be transgenic by efficiently pre-screening transgenic cells and SCNT embryos using the constructed double marker selective vector.

  14. 体细胞核移植技术生产转入溶菌酶基因(hLYZ)牛胚胎的研究%Human Lysozyme Gene (hLYZ) Transgenic Bovine Embryos Produced by Somatic Cell Nuclear Transfer

    Institute of Scientific and Technical Information of China (English)

    熊显荣; 李文哲; 王丽君; 王勇胜; 苏建民; 华松; 张涌

    2011-01-01

    The aim of this study was to investigate the effect of different sizes and treatments of donor cells on the in vitro development of bovine embryos after genetic modification and selection, so as to establish an effective system for production of transgenic bovine embryos.Bovine fetal fibroblasts were transfeected with the recombinant plasmid of mammary gland specific expression vector pEBH which containing human lysozyme (hLYZ) gene.Transgenic positive cells which were obtained through G418 selection were used as donor cells for production of transgenic cloned embryos.The result showed that genetic modification and screening of donor cells were harmful for the development of transgenic cloned embryos, the blastocyst rate decreased significantly;referred to internal diameter of injective needle, cells with the diameter of 15~20 μm were selected and used as nuclear transfer donor cells, the fusion rate and blastocyst rate of transgenic cloned embryos were significant higher than that of the other groups (P < 0.05); Compared with serum starvation and contact restrain groups, the cleavage rate and blastocyst rate of transgenic cloned embryos which were constructed with plant cells were significantly higher (P < 0.05); The expression of GFP was observed in each stage of in vitro development in all transgenic cloned embryos, but the expression levels seemed to be vary among individuals and even among different developmental stages of the same individual.We chose I 0 embroys randomly for PCR detection and found that all of the embroys were positive.Above all, we obtained hLYZ transgenic cloned embryos by somatic cell nuclear transfer technique, the reconstructed embryos can develop to blastocysts successfully; and when plant cells with diameter of 15~20 μm were used as donor cell, the developmental competence of somatic cell cloned embryos is improved significantly.%本研究主要探讨经基因修饰和筛选后转基因供体细胞的挑选及处理对牛转

  15. Cloning by nuclear transfer in the bovine species

    OpenAIRE

    Ectors, Fabien; Delval, Alain; Beckers, Jean-François; Drion, Pierre; Remy, Benoit; Ectors, Francis

    1997-01-01

    Une technique de clonage par transfert nucléaire dont toutes les étapes ont été réalisées exclusivement in vitro a été mise au point. Les embryons reconstitués du premier cycle de clonage ont été utilisés comme donneurs de blastomères pour le second cycle (reclonage). Cette technique nous a permis d'obtenir respectivement 14,5% de morula et 14,9% de blastocystes après le premier et le second cycle de transfert nucléaire. Les pourcentages de naissance obtenus, après transfert de tels embryons,...

  16. Ploidy of Bovine Nuclear Transfer Blastocysts Blastomere Donors

    DEFF Research Database (Denmark)

    Booth, P J; VIUFF, D; THOMSEN, P D;

    2000-01-01

    reconstructed from in vitro produced embryo donors. In vitro matured oocytes were enucleated and then activated using calcium ionophore A23187 followed by 6- dimethylaminopurine (6-DMAP). Subsequently, embryos were reconstructed using blas- tomeres from day 4–5 in vitro produced donors. The embryos were...

  17. Remodeling of the Nuclear Envelope and Lamina during Bovine Preimplantation Development and Its Functional Implications.

    Directory of Open Access Journals (Sweden)

    Jens Popken

    Full Text Available The present study demonstrates a major remodeling of the nuclear envelope and its underlying lamina during bovine preimplantation development. Up to the onset of major embryonic genome activation (MGA at the 8-cell stage nuclei showed a non-uniform distribution of nuclear pore complexes (NPCs. NPCs were exclusively present at sites where DNA contacted the nuclear lamina. Extended regions of the lamina, which were not contacted by DNA, lacked NPCs. In post-MGA nuclei the whole lamina was contacted rather uniformly by DNA. Accordingly, NPCs became uniformly distributed throughout the entire nuclear envelope. These findings shed new light on the conditions which control the integration of NPCs into the nuclear envelope. The switch from maternal to embryonic production of mRNAs was accompanied by multiple invaginations covered with NPCs, which may serve the increased demands of mRNA export and protein import. Other invaginations, as well as interior nuclear segments and vesicles without contact to the nuclear envelope, were exclusively positive for lamin B. Since the abundance of these invaginations and vesicles increased in concert with a massive nuclear volume reduction, we suggest that they reflect a mechanism for fitting the nuclear envelope and its lamina to a shrinking nuclear size during bovine preimplantation development. In addition, a deposit of extranuclear clusters of NUP153 (a marker for NPCs without associated lamin B was frequently observed from the zygote stage up to MGA. Corresponding RNA-Seq data revealed deposits of spliced, maternally provided NUP153 mRNA and little unspliced, newly synthesized RNA prior to MGA, which increased strongly at the initiation of embryonic expression of NUP153 at MGA.

  18. Production of transgenic blastocyst by nuclear transfer from different types of somatic cells in cattle

    Institute of Scientific and Technical Information of China (English)

    GONG Guochun; LI Rong; LI Ning; DAI Yunping; FAN Baoliang; ZHU Huabing; WANG Haiping; WANG Lili; FANG Changge; WAN Rong; LIU Ying

    2004-01-01

    The present study examined the effects of genetic manipulation to the donor cell and different types of transgenic donor cells on developmental potential of bovine nuclear transfer (NT) embryos. Four types of bovine somatic cells, including granulosa cells, fetal fibroblasts, fetal oviduct epithelial cells and fetal ovary epithelial cells, were transfected with a plasmid (pCE-EGFP-Ires-Neo-dNdB) containing the enhanced green fluorescent protein (EGFP) and neomycin-resistant (Neor) genes by electroporation. After 14 days selection with 800 μg/mL G418, transgenic cell lines from each type of somatic cells were obtained. Nontransgenic granulosa cells and all 4 types of transgenic somatic cells were used as nuclear donor to produce transgenic embryos by NT. There was no significant difference in development rates to the blastocyst stage for NT embryos from transgenic and nontransgenic granulosa cells (44.6% and 42.8%, respectively), and transfer of NT embryos derived from transgenic and nontransgenic granulosa cells to recipients resulted in similar pregnancy rates on day 90 (19% and 25%, respectively). The development rates to the blastocyst stage of NT embryos were significantly different among different types of transgenic donor cells (P<0.05). Blastocyst rates from fetal oviduct epithelial cell and granulosa cell (49.1% and 44.6%, respectively) were higher than those from fetal fibroblast (32.7%) and fetal ovary epithelial cell (22.5%). These results suggest that (i) genetic manipulation to donor cells has no negative effect on in vitro and early in vivo developmental competence of bovine NT embryos and (ii) granulosa and fetal oviduct epithelial cells can be used to produce transgenic bovine NT embryos more efficiently. In addition, GFP can be used to select transgenic NT embryos as a non-invasive selective marker.

  19. Factors that affect the reproductive efficiency of the recipient within a bovine embryo transfer program

    Directory of Open Access Journals (Sweden)

    Arturo Duica A.

    2007-12-01

    Full Text Available The embryo transfer is a biotechnological technique that allows increasing the descendant of animals with high genetic value. The positive results, represented in pregnancy after the application of this technique, are affected by some factors that are inherent to the donor, the embryo, the technique, and the recipients which receive a strange embryo in the uterus allowing pregnancy. This review describes some factors affecting the reproductive efficiency of the recipients of bovine embryos within a program of embryo transfer. Its important to evaluate the parameters in this kind of recipients, as race, age, physiological status, health status, weight, reproductive tract integrity and management, and also too monitoring the ovarian structures while the estrus synchronization, and within previous and posterior stages in embryo transfer procedure. Therefore an optimum follicular development will be determinant to corpus luteum formation which generates enough serum progesterone concentrations to offer a right uterine environment allowing the optimum embryo development. Controlling the factors that affect the efficiency of the embryo transfer, it will obtain an increasing of positive results represented in pregnancies and births of individuals come from animals with high genetic value.

  20. Nucleolar remodeling in nuclear transfer embryos

    DEFF Research Database (Denmark)

    Laurincik, Jozef; Maddox-Hyttel, Poul

    2007-01-01

    to develop fully functional nucleoli. In bovine in vivo developed embryos, a range of important nucleolar proteins (e.g., topoisomerase I, upstream binding factor and RNA polymerase I, fibrillarin, nucleophosmin and nucleolin) become localized to the nucleolar anlage over several cell cycles. This relocation...

  1. Controlled ultraviolet resonance energy transfer between bovine serum albumin donors and cadmium sulfide quantum dots acceptors

    Science.gov (United States)

    Ghali, Mohsen; El-Kemary, Maged; Ramadan, Mahmoud

    2015-08-01

    We report on Förester resonance nergy transfer (FRET) within a bioconjugated system composed of cadmium sulfide (CdS) quantum dots (QDs) and transport protein bovine serum albumin (BSA). The optical properties of these two elements of the bioconjugate were exploited to produce FRET in the ultraviolet (UV) region with a maximum efficiency of 22% from BSA donors to QD acceptors. In contrast to previous studies, which were limited to FRET in the visible light, we used 2.6 nm CdS QDs because they emit light with a shorter wavelength (∼370 nm) that facilitates the UV-FRET process. UV-FRET was controlled by tuning the spectral overlap between BSA and CdS QDs.

  2. Somatic Cell Nuclear Transfer in the Mouse

    Science.gov (United States)

    Kishigami, Satoshi; Wakayama, Teruhiko

    Somatic cell nuclear transfer (SCNT) has become a unique and powerful tool for epigenetic reprogramming research and gene manipulation in animals since “Dolly,” the first animal cloned from an adult cell was reported in 1997. Although the success rates of somatic cloning have been inefficient and the mechanism of reprogramming is still largely unknown, this technique has been proven to work in more than 10 mammalian species. Among them, the mouse provides the best model for both basic and applied research of somatic cloning because of its abounding genetic resources, rapid sexual maturity and propagation, minimal requirements for housing, etc. This chapter describes a basic protocol for mouse cloning using cumulus cells, the most popular cell type for NT, in which donor nuclei are directly injected into the oocyte using a piezo-actuated micromanipulator. In particular, we focus on a new, more efficient mouse cloning protocol using trichostatin A (TSA), a histone deacetylase (HDAC) inhibitor, which increases both in vitro and in vivo developmental rates from twofold to fivefold. This new method including TSA will be helpful to establish mouse cloning in many laboratories.

  3. Success in nuclear technology transfer: A Canadian perspective

    International Nuclear Information System (INIS)

    Technology transfer has played a significant part in the expansion of nuclear power to many countries of the world. Canada's involvement in nuclear technology transfer spans four decades. The experience gained through technology transfer, initially to Canadian industry and then to other countries in association with the construction of CANDU nuclear power plants, forms a basis from which to assess the factors which contribute to successful technology transfer. A strong commitment from all parties, in terms of both financial and human resources, is essential to success. Detailed planning of both the scope and timing of the technology transfer program is also required together with an assessment of the impact of the introduction of nuclear power on other sectors of the economy. (author)

  4. Recent Progress of Somatic Cell Nuclear Transfer in Pigs

    Institute of Scientific and Technical Information of China (English)

    Xu Xiaoming; Dou Zhongying

    2005-01-01

    Research in the field of somatic cell nuclear transfer (SCNT) and transgenic cloning in pigs has become a global hotspot, because porcine organs probably can be the first source of donor organs for human xenotransplantation. In recent years, though great progress has been made in porcine SCNT, the efficiency of nuclear transfer remains very low (<1% ). Thus, it is necessary to improve the procedure of nuclear transfer and to investigate some basic problems further. Recent progress and the related problems of SCNT in pigs are reviewed and analyzed so as to offer some beneficial illumination to researchers.

  5. A new MV bus transfer scheme for nuclear power plants

    Directory of Open Access Journals (Sweden)

    Chang Choong-Koo

    2015-01-01

    Full Text Available Fast bus transfer method is the most popular and residual voltage transfer method that is used as a backup in medium voltage buses in general. The use of the advanced technology like open circuit voltage prediction and digital signal processing algorithms can improve the reliability of fast transfer scheme. However, according to the survey results of the recent operation records in nuclear power plants, there were many instances where the fast transfer scheme has failed. To assure bus transfer in any conditions and circumstances, uninterruptible bus transfer scheme utilizing the state of the art medium voltage UPS is discussed and elaborated.

  6. The bovine papillomavirus type 1 E2 transactivator and repressor proteins use different nuclear localization signals.

    Science.gov (United States)

    Skiadopoulos, M H; McBride, A A

    1996-02-01

    The E2 gene of bovine papillomavirus type 1 encodes at least three nuclear phosphoproteins that regulate viral transcription and DNA replication. All three proteins have a common C-terminal domain that has DNA-binding and dimerization activities. A basic region in this domain forms an alpha helix which makes direct contact with the DNA target. In this study, it is shown that in addition to its role in DNA binding, this basic region functions as a nuclear localization signal both in the E2 DNA-binding domain and in a heterologous protein. Deletion of this signal sequence resulted in increased accumulation of the E2 transactivator and repressor proteins in the cytoplasm, but nuclear localization was not eliminated. In the full-length transactivator protein, another signal, present in the N-terminal transactivation domain, is used for transport to the nucleus, and the C-terminal nuclear localization signal(s) are masked. The use of different nuclear localization signals could potentially allow differential regulation of the subcellular localization of the E2 transactivator and repressor proteins at some stage in the viral life cycle. PMID:8551571

  7. Electronic and Nuclear Factors in Charge and Excitation Transfer

    Energy Technology Data Exchange (ETDEWEB)

    Piotr Piotrowiak

    2004-09-28

    We report the and/or state of several subprojects of our DOE sponsored research on Electronic and Nuclear Factors in Electron and Excitation Transfer: (1) Construction of an ultrafast Ti:sapphire amplifier. (2) Mediation of electronic interactions in host-guest molecules. (3) Theoretical models of electrolytes in weakly polar media. (4) Symmetry effects in intramolecular excitation transfer.

  8. Technical knowledge/skill transfer in nuclear power plant manufacturer

    International Nuclear Information System (INIS)

    Due to environmental concerns such as global warming, needs for nuclear power is increasing. However, many expert engineers and technicians are now entering a period of retirement. And due to weak demands of new plant construction for long years, opportunity for technology learning/experience had been lost. Therefore, to secure human resource and to develop their ability are urgent issues for nuclear industries. Hitachi nuclear division continues efforts for technology transfer and human resource training. This paper describes the following two activities. (1) Improvement of common technical basis, and implementation of PDCA cycle, (2) Development of supporting tools to accelerate technology transfer through OJT (On the Job Training). (author)

  9. Heat transfer of nuclear thermal propulsion systems

    Energy Technology Data Exchange (ETDEWEB)

    El-Genk, M.S.; Metzger, J.D.

    1994-12-31

    Nuclear thermal propulsion offers performance capabilities beyond chemical propulsion and is enabling for many planetary missions. Because of the performance capabilities and the number of thermal design issues, NTP offers a productive area for advanced development and research.

  10. Transfer factors for nuclear emergency preparedness

    Energy Technology Data Exchange (ETDEWEB)

    Kostiainen, E.; Haenninen, R. [Radiation and Nuclear Safety Authority (STUK) (Finland); Rosen, K.; Haak, E.; Eriksson, Aa. [Swedish Univ. of Agricultural Science (Sweden); Nielsen, S.P.; Keith-Roach, M. [Risoe National Lab. (Denmark); Salbu, B. [Agricultural Univ. of Norway (Norway)

    2002-12-01

    This report by the NKS/BOK-1.4 project subgroup describes transfer factors for radiocaesium and radiostrontium for the fallout year and the years after the fallout. The intention has been to collect information on tools to assess the order of magnitude of radioactive contamination of agricultural products in an emergency situation in Nordic environment. The report describes transfer paths from fallout to plant, from soil to plant and to animal products. The transfer factors of radionuclides (Sr, Cs, I) given in the report are intended to be used for making rough estimates of the contamination of agricultural products soon after the heaviness and composition of the deposition (Bq m{sup -2}) is known. (au)

  11. Transfer factors for nuclear emergency preparedness

    International Nuclear Information System (INIS)

    This report by the NKS/BOK-1.4 project subgroup describes transfer factors for radiocaesium and radiostrontium for the fallout year and the years after the fallout. The intention has been to collect information on tools to assess the order of magnitude of radioactive contamination of agricultural products in an emergency situation in Nordic environment. The report describes transfer paths from fallout to plant, from soil to plant and to animal products. The transfer factors of radionuclides (Sr, Cs, I) given in the report are intended to be used for making rough estimates of the contamination of agricultural products soon after the heaviness and composition of the deposition (Bq m-2) is known. (au)

  12. Long island to Limerick, nuclear fuel transfer

    International Nuclear Information System (INIS)

    The issue described is: how to move 33 shipments of radioactive nuclear fuel - 200 tons of enriched uranium pellets - on rail cars through the heart of Philadelphia, without upsetting politicians, the media and anti-nuclear activists, after a similar plan to move the fuel through New York City had been rejected in a political disaster. The answer to this is: Strategic Communications Planning. At PECO Energy's department of Corporate and Public Affairs, the research is quite clear that in risk management situations like this, the side that gets out front with the most credible information inevitably wins. That is exactly what was set out to do

  13. Heat transfer and fluid flow in nuclear systems

    CERN Document Server

    Fenech, Henri

    1982-01-01

    Heat Transfer and Fluid in Flow Nuclear Systems discusses topics that bridge the gap between the fundamental principles and the designed practices. The book is comprised of six chapters that cover analysis of the predicting thermal-hydraulics performance of large nuclear reactors and associated heat-exchangers or steam generators of various nuclear systems. Chapter 1 tackles the general considerations on thermal design and performance requirements of nuclear reactor cores. The second chapter deals with pressurized subcooled light water systems, and the third chapter covers boiling water reacto

  14. Cats cloned from fetal and adult somatic cells by nuclear transfer.

    Science.gov (United States)

    Yin, X J; Lee, H S; Lee, Y H; Seo, Y I; Jeon, S J; Choi, E G; Cho, S J; Cho, S G; Min, W; Kang, S K; Hwang, W S; Kong, I K

    2005-02-01

    This work was undertaken in order to study the developmental competence of nuclear transfer (NT) into cat embryos using fetal fibroblast and adult skin fibroblast cells as donor nuclei. Oocytes were recovered by mincing the ovaries in Hepes-buffered TCM199 and selecting the cumulus oocyte complexes (COCs) with compact cumulus cell mass and dark color. Homogenous ooplasm was cultured for maturation in TCM199+10% fetal bovine serum (FBS) for 12 h and used as a source of recipient cytoplast for exogenous somatic nuclei. In experiment 1, we evaluated the effect of donor cell type on the reconstruction and development of cloned embryos. Fusion, first cleavage and blastocyst developmental rate were not different between fetal fibroblasts and adult skin cells (71.2 vs 66.8; 71.0 vs 57.6; 4.0 vs 6.1% respectively; P < 0.05). In experiment 2, cloned embryos were surgically transferred into the oviducts of recipient queens. One of the seven recipient queens was delivered naturally of 2 healthy cloned cats and 1 stillborn from fetal fibroblast cells of male origin 65 days after embryo transfer. One of three recipient queens was delivered naturally of 1 healthy cloned cat from adult skin cells of female origin 65 days after embryo transfer. The cloned cats showed genotypes identical to the donor cell lines, indicating that adult somatic cells can be used for feline cloning. PMID:15695619

  15. Disposal modes for Mars transfer nuclear propulsion

    Science.gov (United States)

    Stancati, Michael L.; Friedlander, Alan L.

    1991-01-01

    A managed disposal approach is proposed that would place the nuclear stage or vehicle in a highly stable orbit at modest cost to mission performance. The approach requires only a small increase in initial mass in LEO, but should be included in preliminary trajectory design and performance calculations. The mass penalty is expected to be larger for all-up flight profiles, or in cases of high-thrust propulsion systems for the cargo vehicle.

  16. Politics of technology transfer (with special reference to the transfer of nuclear technology)

    International Nuclear Information System (INIS)

    A critical analysis is presented of the issues associated with the transfer of technology in general, and nuclear technology in particular, from a socio-political perspective. It is argued that the so-called developing nations are committed to large-scale rapid industrialisation and any interference in the flow of technology to them has serious implications for the future. With their meagre fossil fuel resources they must develop a viable nuclear industry. Industrial nations are accused of manipulating the process of technology transfer as a medium for control and power. In the case of nuclear technology they frequ= entently resort to the issue of non-proliferation to conceal this practice and to unilateral and secretive decision-making. More integrative and liberal institutions should be sought which help promote a wide nuclear technology market to test varying transfer policies and practices. (author)

  17. Association of bovine papillomavirus E2 protein with nuclear structures in vivo.

    Science.gov (United States)

    Kurg, Reet; Sild, Kristiina; Ilves, Aigi; Sepp, Mari; Ustav, Mart

    2005-08-01

    Papillomaviruses are small DNA viruses which have the capacity to establish a persistent infection in mammalian epithelial cells. The papillomavirus E2 protein is a central coordinator of viral gene expression, genome replication, and maintenance. We have investigated the distribution of bovine papillomavirus E2 protein in nuclei of proliferating cells and found that E2 is associated with cellular chromatin. This distribution does not change during the entire cell cycle. The N-terminal transactivation domain, but not the C-terminal DNA-binding domain, of the E2 protein is responsible for this association. The majority of the full-length E2 protein can only be detected in chromatin-enriched fractions but not as a free protein in the nucleus. Limited micrococcal nuclease digestion revealed that the E2 protein partitioned to different chromatin regions. A fraction of the E2 protein was located at nuclear sites that are resistant against nuclease attack, whereas the remaining E2 resided on compact chromatin accessible to micrococcal nuclease. These data suggest that there are two pools of E2 in the cell nucleus: one that localizes on transcriptionally inactive compact chromatin and the other, which compartmentalizes to transcriptionally active nuclear structures of the cell. Our data also suggest that E2 associates with chromatin through cellular protein(s), which in turn is released from chromatin at 0.4 M salt. PMID:16051845

  18. Transfer of industry-oriented nuclear technology at NUCOR

    International Nuclear Information System (INIS)

    The transfer of industry-oriented nuclear technology at the Nuclear Development Corporation of South Africa (Pty) Ltd (NUCOR) is centred in a few divisions only, as most of the NUCOR's program is internally oriented. The industry-oriented activities include radiation technology, production of radioisotopes and application of nuclear techniques in solving problems of industry. The study is concerned mainly with the last of these activities. The general problem of transferring innovative technology is reviewed and a systems approach is used to analyse the transfer process at NUCOR, in terms of the organisation itself and its environment. Organisational strengths and weaknesses are identified and used as a basis to determine opportunities and threats. Possible objectives are formulated and a strategy to meet them is suggested. 'Demand-pull' as opposed to 'technology-push' is advanced as the main triggering mechanism in the transfer of industry-oriented nuclear technology. The importance of marketing this technology, as well as its commercialization, are discussed

  19. Systematic Approach for Nuclear Knowledge Transfer and Preservation in Romania

    International Nuclear Information System (INIS)

    Starting from a thorough analysis of the Nuclear Knowledge Management (NKM) in other countries and based on a realistic study of the status of NKM processes and their efficiency in Romania, the purpose of this project was to improve the knowledge transfer and preservation processes in Romanian nuclear (and support) organizations, especially in INR. The general aim was to extract from our experience the positive and negative aspects and to formulate appropriate recommendations to be integrated into the INR strategies and IAEA guidelines. The project intended: to investigate the positive and negative aspects of the nuclear knowledge transfer and preservation in our country, mainly in our institute, the Institute for Nuclear Research, Pitesti, Romania, during the last 15 years; to underline the tools and methods used to reach this goal, and to analyse the efficiency of these methods; to achieve a general comparison with the situation in East-European countries. The project was based on Romanian historical aspects, desk research on the evolution of the NKM in the East-European countries and investigations in INR research community. Since the explicit knowledge transfer and preservation in INR was early implemented by the means of ISO 9001 practice, our attention was focused on the more delicate aspects of the tacit knowledge transfer. The main research was based on interviews and questionnaires.

  20. 48 CFR 970.4402-4 - Nuclear material transfers.

    Science.gov (United States)

    2010-10-01

    ... 48 Federal Acquisition Regulations System 5 2010-10-01 2010-10-01 false Nuclear material transfers. 970.4402-4 Section 970.4402-4 Federal Acquisition Regulations System DEPARTMENT OF ENERGY AGENCY... is always accomplished, while at the same time minimizing any advantage one party may have over...

  1. Evaluation of a commercial bovine colostrum replacer for achieving passive transfer of immunity in springbok calves (Antidorcas marsupialis).

    Science.gov (United States)

    Thompson, Kimberly A; Lamberski, Nadine; Kass, Philip H; Coons, David; Chigerwe, Munashe

    2013-09-01

    Failure of passive transfer (FPT) occurs in ruminant species when there is inadequate absorption of immunoglobulins from the colostrum. In zoologic establishments, FPT can be a common occurrence in hand-raised ruminant neonates fed insufficient amounts of colostrum replacer (CR) and/or poor-quality CR. The goals of this study were to investigate the efficacy of a commercial bovine CR at achieving adequate passive transfer of immunity and evaluate tests to assess FPT in nondomestic ruminant species. In the past several years, research in dairy calves has shown that passive transfer rates can be dramatically improved if the CR dose is doubled. The treatment group (n = 10) consisted of springbok (Antidorcas marsupialis) calves fed Land O'Lakes bovine commercial CR at a dose of > or = 4.68 g of immunoglobulin G (22 g of CR powder) per kilogram of animal's body weight divided into five feedings over 24 hr. The control group (n=7) consisted of calves that nursed from their dams. This study reported no significant difference between the proportion of calves with adequate passive transfer in the treatment (80%) and control (71%) groups (P= 1.00). Morbidity and mortality rates until weaning were 0% in both groups. The study also determined the sensitivity and specificity values (n = 37) for five serum tests (y-glutamyl-transferase [GGT], globulin, glutaraldehyde coagulation [GC], sodium sulfite turbidity test, and total protein) used to determine passive transfer status in springbok calves. This study recommends the following serum tests and cutoff ranges for determining FPT in springbok calves: globulin or = 28 min. PMID:24063080

  2. Effects of different nuclear recipients on developmental potential of mouse somatic nuclear transfer embryos

    Institute of Scientific and Technical Information of China (English)

    2003-01-01

    In order to investigate the effects of different kinds of nuclear recipients from Kunming (KM) mouse on developmental potential of somatic nuclear transfer em- bryos, the enucleated MⅡ oocytes, enucleated zygotes and 2-cell blastomere were used to produce cloned mouse embryos. Using fibroblast deriving from C57/BL6 ear tissue as nuclear donor, we produced cloned embryos by transferring the fibroblast nuclei into enucleated KM mouse oocytes (single nuclear transfer, SNT), transferring pronuclei from the SNT embryos into enucleated KM zygotes (nuclear into zygote, NZ), and 2-cell blastomere nuclei from SNT embryos into enucleated KM mouse oocytes (nuclear into oocytes, NO); tetraploid embryos (tetraploid embryos, TE) were obtained by fusing two blastomeres, one is from the SNT cloned embryos, and the other from normal 2-cell KM mouse embryos. In group SNT, the cloned embryos could not develop beyond 8-cell stage and the rate of 8-cell stage is only 0.3%; in group NO, the reconstructed embryos could develop to morula stage, the rate of 8-cell stage was significantly greater than that of SNT group (P < 0.05); in group NZ, the development rate was further improved, and the reconstructed embryos could develop into blastocyst stage, the rate of blastocyst was 1.9%; in group TE, as high as 62.3% of the reconstructed embryos could develop into blastocyst. Results suggested that different nuclear recipients could significantly affect the developmental potential of cloned mouse embryos; KM MⅡ oocyte cytoplasm was not so effective as zygotes to reprogram the mouse somatic cell nuclei; serial nuclear transfer could improve the developmental potential of cloned mouse embryos.

  3. Effect of Follicle Size and Follicle Stimulating Hormone Concentration on Nuclear Maturation of Bovine Oocytes In Vitro

    Directory of Open Access Journals (Sweden)

    Uğur Şen

    2015-07-01

    Full Text Available The aim of the study was to investigate the effect of follicle size and follicle stimulating hormone (FSH concentration on nuclear maturation of bovine oocytes in vitro. Follicles on bovine ovary were classified into 3 groups according to the diameter; small (<3 mm, medium (3–8 mm and large (9–12 mm. Oocytes were aspirated from follicles with different size and matured in tissue culture medium (TCM–199 supplemented with 10% FCS and various concentrations of FSH (0.5, 1.0 or 10 and μg/ml for 22 hours filled with approximately 95% humidified and 5% CO2 in air at 38.5 °C. At the end of culture period, nuclear maturation (at metaphase II; MII of oocytes were determined by Bisbenzimide (Hoechst 33258 DNA staining under fluorescent microscope. In the present study, effect of follicle size on nuclear maturation of bovine oocytes were determined and the percentage of oocytes reached to M II stage was significantly lower in oocytes obtained small follicle than those of medium and large follicles. Supplementation of 10.0 μg/ml FSH into maturation media increased percentage of nuclear maturation compare to 0.5 and 1.0 μg/ml. Additionally, improving effect of high FSH concentration on nuclear maturation were more observed in oocytes obtained small follicles. The results of present study showed that oocytes from follicles with 3–8 mm diameters exhibited a more successful maturation, but oocytes obtained small follicles exhibited more maturation as a ratio under high FSH concentration.

  4. Nuclear Effects in Neutrino Interactions at Low Momentum Transfer

    Energy Technology Data Exchange (ETDEWEB)

    Miltenberger, Ethan Ryan [Univ. of Minnesota, Minneapolis, MN (United States)

    2015-05-01

    This is a study to identify predicted effects of the carbon nucleus environment on neutrino - nucleus interactions with low momentum transfer. A large sample of neutrino interaction data collected by the MINERvA experiment is analyzed to show the distribution of charged hadron energy in a region with low momentum transfer. These distributions reveal a major discrepancy between the data and a popular interaction model with only the simplest Fermi gas nuclear effects. Detailed analysis of systematic uncertainties due to energy scale and resolution can account for only a little of the discrepancy. Two additional nuclear model effects, a suppression/screening effect (RPA), and the addition of a meson exchange current process (MEC), are shown to improve the description of the data.

  5. Role of ooplasm in nuclear and nucleolar remodeling of intergeneric somatic cell nuclear transfer embryos during the first cell cycle

    DEFF Research Database (Denmark)

    Østrup, Olga; Strejcek, Frantisek; Petrovicova, Ida;

    2011-01-01

    -cell stage embryos were processed at different points in time post activation (2 hpa, 4 hpa, 8 hpa, and 12 hpa) for detailed nuclear and nucleolar analysis by TEM, and immunofluorescence for visualization of nucleolar proteins related to transcription (UBF) and processing (fibrillarin). Bovine and porcine...

  6. Romanian knowledge transfer network in nuclear physics and engineering - REFIN

    International Nuclear Information System (INIS)

    According to the requirements of the Romanian Nuclear Programme regarding the education and training of the skilled personnel for the nuclear facilities, a knowledge transfer network named REFIN (in Romanian: Retea Educationala in Fizica si Ingineria Nucleara) was developed since 2005. The knowledge target field is nuclear physics and engineering. The Polytechnic University of Bucharest is the coordinator of this programme and other involved partners are University of Bucharest, University of Pitesti, University Babes Bolyai of Cluj-Napoca, University of Constanta, Institute for Nuclear Research Pitesti, Institute for Physics and Nuclear Engineering from Bucharest and the Training Center for Nuclear Units of Cernavoda NPP. The main objective of this network is to develop an effective, flexible and modern educational system in the nuclear physics and engineering area that could meet the requirements of all the known types of nuclear facility and therewith be redundant with the perspectives of the European Research Area (FP7, EURATOM). The first stage in this work was to gather information about the present status in the mentioned nuclear area in Romania, to assimilate and put this information in a data base on the program web-site (www.refin.pub.ro). Based on this data base a global strategy was proposed in order to harmonize the curricula between the network faculties, to implement pilot modern teaching programs (courses/ modules), to introduce advanced learning methods (as Systematic Approach to Training, e-learning and distance-learning), to strengthen and better use of existing research infrastructure of the research institutes in network. The second stage is the investigation of the training stage in other European countries related to the present status and the development trends of education in nuclear field. In the next future the English version will also be available and so REFIN will be easily accessed and used by the interested users. These facilities

  7. Sperm Nuclear Transfer and Transgenic Production in the Fish Medaka

    Directory of Open Access Journals (Sweden)

    Tongming Liu, Ling Liu, Qiwei Wei, Yunhan Hong

    2011-01-01

    Full Text Available Sperm nuclear transfer or intracytoplasmic sperm injection (ICSI is a powerful assisted reproductive technology (ART for treating human male infertility. Controversial reports of increased birth defects have raised concerns about the ART's safety. The cause for birth defects, however, has remained elusive for analysis in human because of the sample size, male infertility genetics, physiological heterogeneity and associated procedures such as embryo manipulations. Animal models are required to evaluate factors leading to the increased birth defects. Here we report the establishment of medakafish model for ICSI and transgenic production. This small laboratory fish has high fecundity and easy embryology. We show that ICSI produced a 5% high percentage of fertile animals that exhibited both paternal and maternal contribution as evidenced by the pigmentation marker. Furthermore, when sperm were pre-incubated with a plasmid ubiquitously expressing RFP and subjected to ICSI, 50% of sperm nuclear transplants showed germline transmission. We conclude that medaka is an excellent model for ICSI to evaluate birth defects and that sperm nuclear transfer can mediate stable gene transfer at high efficiency. Although more demanding for experimentation, sperm-mediated transgenesis should be particularly applicable for aquaculture species with a lengthy generation time and/or a large adult body size.

  8. Expression of a transferred nuclear gene in a mitochondrial genome

    Directory of Open Access Journals (Sweden)

    Yichun Qiu

    2014-08-01

    Full Text Available Transfer of mitochondrial genes to the nucleus, and subsequent gain of regulatory elements for expression, is an ongoing evolutionary process in plants. Many examples have been characterized, which in some cases have revealed sources of mitochondrial targeting sequences and cis-regulatory elements. In contrast, there have been no reports of a nuclear gene that has undergone intracellular transfer to the mitochondrial genome and become expressed. Here we show that the orf164 gene in the mitochondrial genome of several Brassicaceae species, including Arabidopsis, is derived from the nuclear ARF17 gene that codes for an auxin responsive protein and is present across flowering plants. Orf164 corresponds to a portion of ARF17, and the nucleotide and amino acid sequences are 79% and 81% identical, respectively. Orf164 is transcribed in several organ types of Arabidopsis thaliana, as detected by RT-PCR. In addition, orf164 is transcribed in five other Brassicaceae within the tribes Camelineae, Erysimeae and Cardamineae, but the gene is not present in Brassica or Raphanus. This study shows that nuclear genes can be transferred to the mitochondrial genome and become expressed, providing a new perspective on the movement of genes between the genomes of subcellular compartments.

  9. Nuclear transfer to prevent mitochondrial DNA disorders : revisiting the debate on reproductive cloning

    NARCIS (Netherlands)

    Bredenoord, A. L.; Dondorp, W.; Pennings, G.; De Wert, G.

    2011-01-01

    Preclinical experiments are currently performed to examine the feasibility of several types of nuclear transfer to prevent mitochondrial DNA (mtDNA) disorders. Whereas the two most promising types of nuclear transfer to prevent mtDNA disorders, spindle transfer and pronuclear transfer, do not amount

  10. Technological Transfer from Research Nuclear Reactors to New Generation Nuclear Power Reactors

    Science.gov (United States)

    Radulescu, Laura; Pavelescu, Margarit

    2010-01-01

    The goal of this paper is the analysis of the technological transfer role in the nuclear field, with particular emphasis on nuclear reactors domain. The presentation is sustained by historical arguments. In this frame, it is very important to start with the achievements of the first nuclear systems, for instant those with natural uranium as fuel and heavy water as moderator, following in time through the history until the New Generation Nuclear Power Reactors. Starting with 1940, the accelerated development of the industry has implied the increase of the global demand for energy. In this respect, the nuclear energy could play an important role, being essentially an unlimited source of energy. However, the nuclear option faces the challenges of increasingly demanding safety requirements, economic competitiveness and public acceptance. Worldwide, a significant amount of experience has been accumulated during development, licensing, construction, and operation of nuclear power reactors. The experience gained is a strong basis for further improvements. Actually, the nuclear programs of many countries are addressing the development of advanced reactors, which are intended to have better economics, higher reliability, improved safety, and proliferation-resistant characteristics in order to overcome the current concerns about nuclear power. Advanced reactors, now under development, may help to meet the demand for energy power of both developed and developing countries as well as for district heating, desalination and for process heat. The paper gives historical examples that illustrate the steps pursued from first research nuclear reactors to present advanced power reactors. Emphasis was laid upon the fact that the progress is due to the great discoveries of the nuclear scientists using the technological transfer.

  11. Romanian knowledge transfer network in nuclear physics and engineering - REFIN

    International Nuclear Information System (INIS)

    According to the requirements of the Romanian Nuclear Programme regarding the education and training of the skilled personnel for the nuclear facilities, a knowledge transfer network named REFIN (in Romanian: Retea Educationala in Fizica si Ingineria Nucleara) was developed since 2005. The knowledge target field is nuclear physics and engineering. The main objective of this network is to develop an effective, flexible and modern educational system in the nuclear physics and engineering area which could meet the requirements of all known types of nuclear facilities and therewith be redundant with the perspectives of the European Research Area (FP7, EURATOM). A global strategy was proposed in order to harmonize the curricula between the network facilities to implement pilot modern teaching programs (courses/modules), to introduce advanced learning methods (as Systematic Approach to Training, e-learning and distance-learning), to strengthen and better use the existing research infrastructures of the research institutes in network. The education and training strategy is divided into several topics: university engineering , master, post-graduate, Ph.D. degree, post-doctoral activity, training for industry, improvement. For the first time in our country, a modular scheme is used allowing staff with different technical background to participate at different levels. In this respect, the European system with transferable credits (ECTS) is used. Based on this strategy, courses in 'Radioactive Waste Management' and 'Numerical and Experimental Methods in Reactor Physics' for both MS students and for industry. This way the training activity which a student attends will allow him or her to be involved, depending on specific professional needs, into a flexible educational scheme. This scheme will ensure competence and enhancement and also the possibility of qualification development and a better mobility on labour market. This kind of activity is already in progress in the

  12. Estimating transfer of bovine virus-diarrhoea virus in Danish cattle by use of register data

    DEFF Research Database (Denmark)

    Alban, L.; Stryhn, H.; Kjeldsen, A.M.;

    2001-01-01

    To study how routinely recorded data (also called "register data") might be used in disease monitoring on a regional or national level, a database for bovine virus-diarrhoea virus (BVDV) was made from existing databases, covering the period January 1995-November 1999. This paper includes a general...... description of the database, including basic statistics for selected variables. Information was largely complete for cattle herds in the milk-recording scheme (MRS), but only partly available for other herds. A methodology was developed to identify when and how a herd initially was infected. For most herds...

  13. The project of Esfahan Nuclear Technology Center (ENTEC) and the transfer of nuclear tecnology in Iran

    International Nuclear Information System (INIS)

    In 1974, the Atomic Energy Organization of Iran (AEOI) decided to set up a Nuclear Technology Center on Esfahan (ENTEC) in collaboration with France's Technicatome Company and the CEA. This center is scheduled to go into operation during 1976-1980. The purposes for setting up ENTEC are threefold: a. to give scientific and technical support to the operation of nuclear power plants and nuclear industries in Iran. b. to carry out research and development in the area of nuclear technology on an industrial level. c. to give supplementary education and training to the manpower needs for the AEOI. To carry out the program of technology transfer, temporary laboratories have been set up in Tehran for engineers, technicians and training programs have been organized primarily in France. The ENTEC project will also include a school for education of junior scientists and engineers in the field of nuclear technology

  14. Heat transfer in nuclear fuels: Measurements of gap conductance

    Science.gov (United States)

    Cho, Chun Hyung

    Heat transfer in the fuel-clad gap in a nuclear reactor impacts the overall temperature distribution, stored energy and the mechanical properties of a nuclear fuel rod. Therefore, an accurate estimation of the gap conductance between the fuel and the clad is critically important for reactor design and operations. To obtain the requisite accuracy in the gap conductance estimation, it is important to understand the effects of the convective heat transfer coefficient, the gas composition, pressure and temperature, and so forth. The objectives of this study are to build a bench-scale experimental apparatus for the measurement of thermal gap conductances and to develop a better understanding of the differences that have been previously observed between such measured values and those predicted theoretically. This is accomplished by employing improved analyses of the experiments and improved theoretical models. Using laser heating of slightly separated stainless-steel plates, the gap conductance was measured using a technique that compares the theoretical and experimental time dependent temperatures at the back surface of the second plate. To consider the effects of surface temperature and gas pressure, the theoretical temperatures were calculated using a convective heat transfer coefficient that was dependent upon both the temperature and the gas pressure.

  15. Animal embryonic stem (ES) cells: self-renewal, pluripotency, transgenesis and nuclear transfer.

    Science.gov (United States)

    Saito, Shigeo; Liu, Bingbing; Yokoyama, Kazunari

    2004-09-01

    Mouse embryonic stem (ES) cells can be maintained indefinitely in the presence of leukemia inhibitory factor (LIF) and they express markers of self-renewal and pluripotency, which include the transcription factor Oct 4, STAT-3, stage-specific embryonic antigen (SSEA)-1, and alkaline phosphatase (AP). Upon removal of LIF, from the culture medium they cease to express markers such as Oct 4, rapidly losing the capacity for self-renewal and differentiating into a variety of cell types. Gene targeting is feasible in murine ES cells because these cells can be maintained in an undifferentiated state long enough to allow selection of properly targeted cell colonies with a high frequency of homologous recombination. Furthermore, blastocysts cloned from cultured murine ES cells develop to term at an efficiency (10-30%) that is three to ten times higher than blastocysts cloned from the nuclei of differentiated somatic cells. It seems likely that ES cells require less extensive reprogramming than do somatic cells, perhaps because in ES cells, many genes that are essential for early development are already active and thus do not require reactivation. Recently, we succeeded in isolating immortalized equine and bovine ES cells with a normal karyotype, that exhibit features similar to those of murine ES cells and express Oct 4, STAT-3, SSEA-1 and AP. We further confirmed the pluripotential ability of these cells, which were able to undergo somatic differentiation in vitro to neural progenitors and to endothelial or hematopoietic lineages. We were able to use bovine ES cells, as a source of nuclei for nuclear transfer (NT) and we generated cloned cattle with a higher frequency of pregnancies to term than has been achieved with differentiated somatic cells. Moreover, bovine ES cells that expressed enhanced green fluorescent protein (EGFP) were incorporated into both the inner cell mass (ICM) and the trophectdermal cells of developing blastocysts. These findings should facilitate

  16. Serial Nuclear Transfer of Goat-Rabbit Interspecies Reconstructed Embryos

    Institute of Scientific and Technical Information of China (English)

    ZHANG Zhi-guo; CHENG Li-zi; ZHANG Xiao-rong; LIU Ya; JING Ren-tao; WANG Cun-li; ZHAO Huan; LI Bin; CAO Chen-chong; LI Dong-wei

    2005-01-01

    The experiments of serial nuclear transfer were conducted between Boer goat and rabbit. The enucleated oocytes of rabbit were used as recipients while the blastomeres of goat morula was used as nuclear donor. The reconstructed embryos developing to morula were used as donor for serial cloning. As a result, two generations of reconstructed embryos were obtained, including 58 first generation reconstructed embryos and 14 second generation reconstructed embryos. The fusion rates were 79.5 and 70%, respectively. and there was no significant difference between them (P>0.05). The cleavage rates were 75.9 and 28.6% respectively with significant difference (P<0.01). No blastocyst was obtained from the second generation reconstructed embryos while 13.8% of first generation reconstructed embryos developed to blastocyst.

  17. Technology transfer programs using a low power nuclear reactor

    International Nuclear Information System (INIS)

    The SLOWPOKE II nuclear reactor developed by Atomic Energy of Canada Limited is well suited for neutron activation analysis and the production of small quantities of radionuclides. Emphasis has been placed on local research groups to transfer appropriate technology developed in their laboratories into the community. The development of several research protocols and associated technology is reviewed and their successful implementation into local industry is outlined. These include for example, the monitoring of environmental chlorinated compounds, the irradiation of gem stones, placer gold-mining efficiency measurements and measuring industrial flow-processes. (author) 6 refs.; 1 tab

  18. Mapping of nuclear import signal and importin {alpha}3 binding regions of 52K protein of bovine adenovirus-3

    Energy Technology Data Exchange (ETDEWEB)

    Paterson, Carolyn P.; Ayalew, Lisanework E. [Vaccine and Infectious Disease Organization-International Vaccine Center (VIDO-InterVac), University of Saskatchewan, Saskatoon, SK S7N 5E3 Canada (Canada); Veterinary Microbiology, University of Saskatchewan, Saskatoon, SK S7N 5E3 S7N 5B4 Canada (Canada); Tikoo, Suresh K., E-mail: suresh.tik@usask.ca [Vaccine and Infectious Disease Organization-International Vaccine Center (VIDO-InterVac), University of Saskatchewan, Saskatoon, SK S7N 5E3 Canada (Canada); Veterinary Microbiology, University of Saskatchewan, Saskatoon, SK S7N 5E3 S7N 5B4 Canada (Canada); School of Public Health, University of Saskatchewan, Saskatoon, SK S7N 5E5 Canada (Canada)

    2012-10-10

    The L1 region of bovine adenovirus (BAdV)-3 encodes a non-structural protein designated 52K. Anti-52K serum detected a protein of 40 kDa, which localized to the nucleus but not to the nucleolus in BAdV-3-infected or transfected cells. Analysis of mutant 52K proteins suggested that three basic residues ({sup 105}RKR{sup 107}) of the identified domain (amino acids {sup 102}GMPRKRVLT{sup 110}) are essential for nuclear localization of 52K. The nuclear import of a GST-52K fusion protein utilizes the classical importin {alpha}/{beta}-dependent nuclear transport pathway. The 52K protein is preferentially bound to the cellular nuclear import receptor importin {alpha}3. Although deletion of amino acid 102-110 is sufficient to abrogate the nuclear localization of 52K, amino acid 90-133 are required for interaction with importin-{alpha}3 and localizing a cytoplasmic protein to the nucleus. These results suggest that 52K contains a bipartite NLS, which preferentially utilize an importin {alpha}3 nuclear import receptor-mediated pathway to transport 52K to the nucleus.

  19. Formation of chambers in the bovine uterus during early pregnancy. A comparison between morphological, ultrasonic and nuclear magnetic resonance findings

    International Nuclear Information System (INIS)

    Formation of chambers in the bovine uterus during early pregnancy A comparison between morphological, ultrasonic and nuclear magnetic resonance findings In the bovine, the uterus shows prominent circular folds during early pregnancy. These folds protrude into the lumen nearly at right angles to the uterine wall and, reaching a height of about 2–3 cm in some cases, often reach the center of the uterine lumen. Thus the uterus becomes apparently divided into a number of pocket-like chambers. Endometrial folds are usually found in the pregnant as well as the contralateral uterine horn. Intra-uterine features can be well demonstrated on exenterated bovine uteri without injuring the organ by means of nuclear magnetic resonance imaging with a high resolutive performance. This technique was used to examine the uteri of 4 heifers on day 26, 28, 32 and 55 of pregnancy, respectively. The 3-dimensional imaging of these uteri demonstrated the presence of crescent-shaped folds usually at right angles to the long axis of the uterine horn. These endometrial folds explain why the allantochorionic vesicle cannot be demonstrated in toto by transrectal sonography during early pregnancy, apparently being subdivided by the protrusions from the uterine wall. It has been found that the uterus is characterized by 2–3 echo-weak compartments around day 25 of pregnancy and by about 4–6 by day 30. The number may yet increase with progressing pregnancy. Histological sections out of the wall of the pregnant and the contralateral uterine horn have revealed that the folds are composed mainly of the endometrium (Tunica mucosa) and the circular muscle (Stratum circulare)

  20. Förster resonance energy transfer between pyrene and bovine serum albumin: Effect of the hydrophobic pockets of cyclodextrins

    Science.gov (United States)

    Maity, Arnab; Mukherjee, Puspal; Das, Tarasankar; Ghosh, Prasun; Purkayastha, Pradipta

    The phenomenon of Förster resonance energy transfer (FRET) between pyrene and bovine serum albumin (BSA) protein in presence of cyclodextrins (CDs) is explored in the present work. CDs provide hydrophobic environment and thus the aromatic molecules get encapsulated in them depending on the relative size and space. In this work we revealed that along with pyrene monomer, the side chains of amino acids in BSA can get trapped partly in the hydrophobic cavities of CDs if space permits. While being encapsulated by β-CD as pyrene monomer, it can interact with the BSA tryptophan moiety exposed toward the aqueous environment to form a dimer through π-π interaction. This, in turn, affects the energy transfer process by reducing the efficiency. On the other hand, pyrene excimer gets encapsulated in a γ-CD molecule due to availability of enough space. The excimer shows a new band at a higher wavelength. This further reduces FRET efficiency due to scarcity of acceptor for the tryptophan moieties in BSA.

  1. Transfer of nuclear technology to the developing countries

    International Nuclear Information System (INIS)

    The increased Agency assistance for transfer of nuclear technology is essential for the developing countries and especially Africa. It would have a beneficial effect on the implementation of training programmes. The introduction of teaching in nuclear physics at universities in Nigeria, Tanzania and Madagascar should be extended to other universities in order further to orientate African students towards nuclear sciences. In the peaceful uses of atomic energy the African States are concentrating their activities in the spheres of agriculture and medicine. The Agency assists these countries in programmes in agriculture and the exploitation of natural resources, including water. The introduction of radioisotope techniques should be accelerated at all existing agricultural research centres. Services of this kind exist in a few countries, including Senegal, Kenya, the Ivory Coast, Morocco and the Sudan. Radioisotopes employed there make it possible, in particular, to trace the movement of fertilizers from soil to plant and to measure soil humidity. Ionizing radiations can be used to produce genetic effects for the purpose of creating hew varieties of important crop plants and selecting varieties requiring less water for their growth. Such activities are naturally of the greatest interest to the African continent which, as a whole, lives basically from agriculture. The guarantee of food supplies during the next decade is a subject of concern for Africa, and the prevention of losses of foodstuffs is one of the main objectives of African policy.Food irradiation projects are being conducted in Ghana (on cocoa beans) and in Nigeria (on sweet potatoes) with the support of Agency research projects. The Agency's project on the radiation preservation of fish, at present being implemented in countries in Asia, could - provided that the results are satisfactory - have important economic repercussions for the African countries.In the medical sphere, the improvement in health

  2. Association of Bovine Papillomavirus E2 Protein with Nuclear Structures In Vivo

    OpenAIRE

    Kurg, Reet; Sild, Kristiina; Ilves, Aigi; Sepp, Mari; Ustav, Mart

    2005-01-01

    Papillomaviruses are small DNA viruses which have the capacity to establish a persistent infection in mammalian epithelial cells. The papillomavirus E2 protein is a central coordinator of viral gene expression, genome replication, and maintenance. We have investigated the distribution of bovine papillomavirus E2 protein in nuclei of proliferating cells and found that E2 is associated with cellular chromatin. This distribution does not change during the entire cell cycle. The N-terminal transa...

  3. Effect of freeze-thaw cycles on load transfer between the biomineral and collagen phases in bovine dentin

    International Nuclear Information System (INIS)

    Stabilization of biological materials by freezing is widespread in the fields of medicine and biomaterials research and yet, in the case of hard biomaterials such as dentin, there is not a good understanding of how such treatments might affect the mechanical properties. The freezing and thawing may have a number of different effects on dentin including formation of cracks in the microstructure and denaturation of the collagen. Using high-energy synchrotron X-ray diffraction, the apparent moduli of bovine dentin samples were measured before and after various numbers of freeze-thaw cycles. It was determined that repeated freezing and thawing has no measurable effect on the hydroxyapatite or fibrillar apparent moduli up to 10 cycles. This confirms that the use of low temperature storage for stabilization of dentin is reasonable in cases where stiffness is a property of importance. Highlights: → Studied the effect of freezing on the load transfer of HAP and fibrils in dentin. → X-ray scattering measured HAP and fibril apparent moduli vs. freezing cycles. → Apparent moduli did not vary significantly between 0 and 10 freeze thaw cycles. → Residual strains imply no widespread cracking due to volumetric expansion of water. → Dentin can be freeze-thawed with no significant effects on elastic properties.

  4. Creating genetically modified pigs by using nuclear transfer

    Directory of Open Access Journals (Sweden)

    Prather Randall S

    2003-11-01

    Full Text Available Abstract Nuclear transfer (NT is a procedure by which genetically identical individuals can be created. The technology of pig somatic NT, including in vitro maturation of oocytes, isolation and treatment of donor cells, artificial activation of reconstructed oocytes, embryo culture and embryo transfer, has been intensively studied in recent years, resulting in birth of cloned pigs in many labs. While it provides an efficient method for producing transgenic pigs, more importantly, it is the only way to produce gene-targeted pigs. So far pig cloning has been successfully used to produce transgenic pigs expressing the green fluorescence protein, expand transgenic pig groups and create gene targeted pigs which are deficient of alpha-1,3-galactosyltransferase. The production of pigs with genetic modification by NT is now in the transition from investigation to practical use. Although the efficiency of somatic cell NT in pig, when measured as development to term as a proportion of oocytes used, is not high, it is anticipated that the ability of making specific modifications to the swine genome will result in this technology having a large impact not only on medicine but also on agriculture.

  5. Using somatic-cell nuclear transfer to study aging.

    Science.gov (United States)

    Kishigami, Satoshi; Lee, Ah Reum; Wakayama, Teruhiko

    2013-01-01

    In mammals, a diploid genome following fertilization of haploid cells, an egg, and a spermatozoon is unique and irreproducible. This implies that the generated unique diploid genome is doomed with the individual's inevitable demise. Since it was first reported in 1997 that Dolly the sheep had been cloned, many mammalian species have been cloned successfully using somatic-cell nuclear transfer (SCNT). The success of SCNT in mammals enables us not only to reproduce offspring without germ cells, that is, to "passage" a unique diploid genome, but also to address valuable biological questions on development, nuclear reprogramming, and epigenetic memory. Successful cloning can also support epigenetic reprogramming where the aging clock is reset or reversed. Recent work using iPS cell technology has explored the practicality and led to the recapitulation of premature aging with iPSCs from progeroid laminopathies. As a result, reprogramming tools are also expected to contribute to studying biological age. However, the efficiency of animal cloning is still low in most cases and the mechanism of reprogramming in cloned embryos is still largely unclear. Here, based on recent advances, we describe an improved, more efficient mouse cloning protocol using histone deacetylase inhibitors (HDACis) and latrunculin A, which increases the success rates of producing cloned mice or establishing ES cells fivefold. This improved method of cloning will provide a strong tool to address many issues including biological aging more easily and with lower cost. PMID:23929101

  6. Gnotobiotic Miniature Pig Interbreed Somatic Cell Nuclear Transfer for Xenotransplantation.

    Science.gov (United States)

    Hwang, Jeong Ho; Kim, Sang Eun; Gupta, Mukesh Kumar; Lee, HoonTaek

    2016-08-01

    Transgenic animal producing technology has improved consistently over the last couple of decades. Among the available methods, somatic cell nuclear transfer (SCNT) technology was officially the most popular. However, SCNT has low efficiency and requires a highly skilled individual. Additionally, the allo-SCNT nuclear reprogramming mechanism is poorly understood in the gnotobiotic miniature pig, which is a candidate for xenotransplantation, making sampling in oocytes very difficult compared to commercial hybrid pigs. Therefore, interbreed SCNT (ibSCNT), which is a combination of miniature pig and commercial pig (Landrace based), was analyzed and was found to be similar to SCNT in terms of the rate of blastocyst formation (12.6% ± 2.9% vs. 15.5% ± 2.2%; p > 0.05). However, a significantly lower fusion rate was observed in the ibSCNT compared to normal SCNT with Landrace pig somatic cells (29.6% ± 0.8% vs. 65.0% ± 4.9%). Thus, the optimization of fusion parameters was necessary for efficient SCNT. Our results further revealed that ibSCNT by the whole-cell intracytoplasmic injection (WCICI) method had a significantly higher blastocyst forming efficiency than the electrofusion method (31.1 ± 8.5 vs. 15.5% ± 2.2%). The nuclear remodeling and the pattern of changes in acetylation at H3K9 residue were similar in both SCNT and ibSCNT embryos. PMID:27459580

  7. MODELAGEM BIOECONÔMICA DA TRANSFERÊNCIA DE EMBRIÕES EM BOVINOS BIOECONOMIC MODEL IN BOVINE EMBRYO TRANSFER

    Directory of Open Access Journals (Sweden)

    Renato Travassos Beltrame

    2010-04-01

    Full Text Available

    O objetivo deste trabalho foi desenvolver um modelo matemático orientado a eventos de simulação, para auxiliar tomadas de decisão relativas à transferência de embriões em bovinos, considerando-se as dinâmicas de dois componentes da transferência de embriões: receptoras e embriões. Na simulação, não se avaliaram respostas individuais de doadoras a coletas consecutivas e eventos correspondentes na transferência de embriões. Simulou-se o mesmo protocolo para superovulação a todas as doadoras. Receptoras foram sincronizadas simulando-se o uso de prostaglandina. O número de embriões viáveis produzido por doadora e sua variabilidade tiveram como base um processo aleatório de simulação de Monte Carlo, que pressupôs uma distribuição exponencial negativa de densidade de probabilidade. Custos e receitas foram inseridos no modelo por meio de um cenário-base para calcular indicadores econômicos de rentabilidade. A análise sugeriu a impraticabilidade da atividade, se realizada diante do cenário proposto (VPL – R$: 57.596,69. A partir do cenário proposto, o custo médio estimado foi de R$ 1.178,19, e de R$ 980,03, para se obter uma prenhez a partir de uma situação otimizada, sugerida pelo modelo (5/100; 5/190.

    PALAVRAS-CHAVES: Otimização, receptoras, simulação, transferência de embriões, viabilidade econômica.

    A simulation model related to embryo transfer programs in bovine was carried out through a mathematical model directed to events, considering the dynamic of two resources: recipients and embryos. Individual answers of donors to consecutive collections and corresponding events in embryo transfer were not evaluated. The same protocol for superovulation was simulated for all the donor collections, using similar doses of hormones and drugs for all the animals. Recipients were synchronized using prostaglandin. Meantime, the number of viable embryos produced by donor and its variability were based at

  8. Differentiation-specific action of orphan nuclear receptor NR5A1 (SF-1): transcriptional regulation in luteinizing bovine theca cells

    OpenAIRE

    Walther, Norbert; Jansen, Martina; Akbary, Wasima; Ivell, Richard

    2006-01-01

    Background The orphan nuclear receptor NR5A1 (steroidogenic factor-1, SF-1) is a master regulator of tissue-specific gene expression in reproductive and steroidogenic tissues. Two activating functions, AF-1 and AF-2, have been described to function in a cooperative manner to recruit transcriptional coactivators to the promoter regions of NR5A1-controlled genes. Methods The role of the NR5A1 activating functions AF-1 and AF-2 was studied in primary bovine theca cells. Bovine theca cells were i...

  9. Characteristics of pregnancies and offspring following transfer of bovine in vivo embryos assessed by nanorespirometry

    DEFF Research Database (Denmark)

    Lopes, Ana Sofia; Madsen, S E; Greve, Torben;

    2008-01-01

    It has been speculated whether the metabolism of the pre-implantation embryo may be reflected on the pregnancy and characteristics of the newborn animal. The present study investigated whether respiration rates of individual embryos were correlated with gestation length, type of parturition, birth......, III), stage of development, and diameter and were subsequently transferred individually (n = 43) to synchronized recipients. Gestation length of the recipients (n = 22) was calculated and the type of parturition (no assistance, light traction, heavy traction, or caesarean section) recorded. Sex......, weight, and condition of the calves at birth (weak, normal, or very active) were also assessed. Results were evaluated by chi-square analysis and using a linear mixed model. The pregnancy rate was 60% (26/43), and the respiration rates of individual embryos influenced gestation length as well...

  10. Nuclear Transparency in Large Momentum Transfer Quasielastic Scattering

    International Nuclear Information System (INIS)

    We measured simultaneously pp elastic and quasielastic (p,2p) scattering in hydrogen, deuterium, and carbon for momentum transfers of 4.8 to 6.2 (GeV/c)2 at incoming momenta of 5.9 and 7.5 GeV/c and center-of-mass scattering angles in the range θc.m.=83.7 degree - 90 degree . The nuclear transparency is defined as the ratio of the quasielastic cross section to the free pp cross section. At incoming momentum of 5.9 GeV/c , the transparency of carbon decreases by a factor of 2 from θc.m.≅85 degree to θc.m.≅89 degree . At the largest angle the transparency of carbon increases from 5.9 to 7.5 GeV/c by more than 50%. The transparency in deuterium does not depend on incoming momentum nor on θc.m. . copyright 1998 The American Physical Society

  11. Natural convection heat transfer within horizontal spent nuclear fuel assemblies

    Energy Technology Data Exchange (ETDEWEB)

    Canaan, R.E.

    1995-12-01

    Natural convection heat transfer is experimentally investigated in an enclosed horizontal rod bundle, which characterizes a spent nuclear fuel assembly during dry storage and/or transport conditions. The basic test section consists of a square array of sixty-four stainless steel tubular heaters enclosed within a water-cooled rectangular copper heat exchanger. The heaters are supplied with a uniform power generation per unit length while the surrounding enclosure is maintained at a uniform temperature. The test section resides within a vacuum/pressure chamber in order to subject the assembly to a range of pressure statepoints and various backfill gases. The objective of this experimental study is to obtain convection correlations which can be used in order to easily incorporate convective effects into analytical models of horizontal spent fuel systems, and also to investigate the physical nature of natural convection in enclosed horizontal rod bundles in general. The resulting data consist of: (1) measured temperatures within the assembly as a function of power, pressure, and backfill gas; (2) the relative radiative contribution for the range of observed temperatures; (3) correlations of convective Nusselt number and Rayleigh number for the rod bundle as a whole; and (4) correlations of convective Nusselt number as a function of Rayleigh number for individual rods within the array.

  12. Natural convection heat transfer within horizontal spent nuclear fuel assemblies

    International Nuclear Information System (INIS)

    Natural convection heat transfer is experimentally investigated in an enclosed horizontal rod bundle, which characterizes a spent nuclear fuel assembly during dry storage and/or transport conditions. The basic test section consists of a square array of sixty-four stainless steel tubular heaters enclosed within a water-cooled rectangular copper heat exchanger. The heaters are supplied with a uniform power generation per unit length while the surrounding enclosure is maintained at a uniform temperature. The test section resides within a vacuum/pressure chamber in order to subject the assembly to a range of pressure statepoints and various backfill gases. The objective of this experimental study is to obtain convection correlations which can be used in order to easily incorporate convective effects into analytical models of horizontal spent fuel systems, and also to investigate the physical nature of natural convection in enclosed horizontal rod bundles in general. The resulting data consist of: (1) measured temperatures within the assembly as a function of power, pressure, and backfill gas; (2) the relative radiative contribution for the range of observed temperatures; (3) correlations of convective Nusselt number and Rayleigh number for the rod bundle as a whole; and (4) correlations of convective Nusselt number as a function of Rayleigh number for individual rods within the array

  13. Nuclear Technology Series. Course 4: Heat Transfer and Fluid Flow.

    Science.gov (United States)

    Center for Occupational Research and Development, Inc., Waco, TX.

    This technical specialty course is one of thirty-five courses designed for use by two-year postsecondary institutions in five nuclear technician curriculum areas: (1) radiation protection technician, (2) nuclear instrumentation and control technician, (3) nuclear materials processing technician, (4) nuclear quality-assurance/quality-control…

  14. Embryo aggregation does not improve the development of interspecies somatic cell nuclear transfer embryos in the horse.

    Science.gov (United States)

    Gambini, Andrés; De Stéfano, Adrián; Jarazo, Javier; Buemo, Carla; Karlanian, Florencia; Salamone, Daniel Felipe

    2016-09-01

    The low efficiency of interspecies somatic cell nuclear transfer (iSCNT) makes it necessary to investigate new strategies to improve embryonic developmental competence. Embryo aggregation has been successfully applied to improve cloning efficiency in mammals, but it remains unclear whether it could also be beneficial for iSCNT. In this study, we first compared the effect of embryo aggregation over in vitro development and blastocyst quality of porcine, bovine, and feline zona-free (ZF) parthenogenetic (PA) embryos to test the effects of embryo aggregation on species that were later used as enucleated oocytes donors in our iSCNT study. We then assessed whether embryo aggregation could improve the in vitro development of ZF equine iSCNT embryos after reconstruction with porcine, bovine, and feline ooplasm. Bovine- and porcine-aggregated PA blastocysts had significantly larger diameters compared with nonaggregated embryos. On the other hand, feline- and bovine-aggregated PA embryos had higher blastocyst cell number. Embryo aggregation of equine-equine SCNT was found to be beneficial for embryo development as we have previously reported, but the aggregation of three ZF reconstructed embryos did not improve embryo developmental rates on iSCNT. In vitro embryo development of nonaggregated iSCNT was predominantly arrested around the stage when transcriptional activation of the embryonic genome is reported to start on the embryo of the donor species. Nevertheless, independent of embryo aggregation, equine blastocyst-like structures could be obtained in our study using domestic feline-enucleated oocytes. Taken together, these results reported that embryo aggregation enhance in vitro PA embryo development and embryo quality but effects vary depending on the species. Embryo aggregation also improves, as expected, the in vitro embryo development of equine-equine SCNT embryos; however, we did not observe positive effects on equine iSCNT embryo development. Among oocytes

  15. Comparative genomics and the role of lateral gene transfer in the evolution of bovine adapted Streptococcus agalactiae

    OpenAIRE

    Richards, Vincent P; Lang, Ping; Bitar, Paulina D. Pavinski; Lefebure, Tristan; Schukken, Ynte H.; Zadoks, Ruth N.; Michael J Stanhope

    2011-01-01

    In addition to causing severe invasive infections in humans, Streptococcus agalactiae, or group B Streptococcus (GBS), is also a major cause of bovine mastitis. Here we provide the first genome sequence for S. agalactiae isolated from a cow diagnosed with clinical mastitis (strain FSL S3-026). Comparison to eight S. agalactiae genomes obtained from human disease isolates revealed 183 genes specific to the bovine strain. Subsequent polymerase chain reaction (PCR) screening for the presence/abs...

  16. The World Nuclear University Summer Institute - A Forum for Nuclear Knowledge Transfer and Leadership

    International Nuclear Information System (INIS)

    Education and training involve passing knowledge to the next generation and sharing knowledge for capacity building. Education in this context denotes higher education received in universities etc., whereas training denotes transfer of shorter, skill oriented specialized knowledge, which is usually done by the nuclear industry or organizations. Thus, education and training are considered important tools for preserving and sustaining knowledge. Recently, networking of educational institutions has been considered a key strategy for capacity building and efficient use of available educational resources. The strength of the IAEA lies in its ability to propose and facilitate frameworks in which Member States can work together and collaborate. The idea of the WNU was presented at the IAEA for the first time during various IAEA meetings in 2001 and 2002. The Department of Nuclear Energy was involved in most of this work through its sub-programme on Nuclear Knowledge Management. The World Nuclear University is a global partnership committed to enhancing international education and leadership in the peaceful applications of nuclear science and technology. The central elements of the WNU partnership are: - The global organizations of the nuclear industry: World Nuclear Association (WNA) and World Association of Nuclear Operators (WANO); - The inter-governmental nuclear agencies: IAEA and Nuclear Energy Agency (NEA) of the Organization for Economic Cooperation and Development (OECD); - Leading institutions of nuclear learning in some thirty countries. The WNU was inaugurated in 2003 in a London ceremony commemorating the 50th anniversary of President Eisenhower's historic Atoms for Peace initiative; the visionary proposal that gave birth to the IAEA. This United Nations specialized agency is one of the four world organizations known as the WNU's 'Founding Supporters'. Within the United Nations system, the WNU is recognized as a 'Partnership for Sustainable Development' by the

  17. 牛胚胎移植技术的产业化研究%Study of Industrilization of Bovine Embryo Transferring

    Institute of Scientific and Technical Information of China (English)

    冯建忠; 史远刚; 张秀陶; 梁小军; 薛伟; 扬奇; 孟华; 李毓华

    2001-01-01

    Embryo transfer and relative technology was explicated, including setting up of nucleus herd of cattle by importing frozen embryo, choice of donor, superovalution, cryopreservation of embryo and embryo splitting. This study make a base for the industrilization of bovine embryo transferring.%本文探讨了引进国外优质奶、肉牛冷冻胚胎建立核心群,以及供体牛选择、超数排卵、胚胎冷冻保存和分割等相关技术,为牛胚胎移植产业化奠定基础。

  18. Identification of nuclear effects in neutrino-carbon interactions at low three-momentum transfer

    CERN Document Server

    Rodrigues, P A; Miltenberger, E; Aliaga, L; Altinok, O; Bercellie, A; Betancourt, M; Bodek, A; Bravar, A; Carneiro, M F; Chvojka, J; Devan, J; Eberly, B; Elkins, M; Felix, J; Fields, L; Fine, R; Gago, A M; Galindo, R; Gallagher, H; Ghosh, A; Golan, T; Gran, R; Harris, D A; Higuera, A; Hurtado, K; Kiveni, M; Kleykamp, J; Kordosky, M; Le, T; Leistico, J R; Lovlein, A; Maher, E; Manly, S; Mann, W A; Marshall, C M; Caicedo, D A Martinez; McFarland, K S; McGivern, C L; McGowan, A M; Messerly, B; Miller, J; Mislivec, A; Mousseau, J; Muhlbeier, T; Naples, D; Nelson, J K; Norrick, A; Nuruzzaman,; Patrick, C E; Perdue, G N; Ramirez, M A; Ransome, R D; Ray, H; Ren, L; Rimal, D; Ruterbories, D; Schmitz, D W; Salinas, C J Solano; Tice, B G; Valencia, E; Walton, T; Wolcott, J; Wospakrik, M; Zavala, G; Zhang, D

    2015-01-01

    Two different nuclear-medium effects are isolated using a low three-momentum transfer subsample of neutrino-carbon scattering data from the MINERvA neutrino experiment. The observed hadronic energy in charged-current $\

  19. Heat Transfer Phenomena in Supercritical Water Nuclear Reactors

    International Nuclear Information System (INIS)

    A supercritical water heat transfer facility has been built at the University of Wisconsin to study heat transfer in a circular and square annular flow channel. A series of integral heat transfer measurements has been carried out over a wide range of heat flux, mass velocity and bulk water temperatures at a pressure of 25 MPa. The circular annular test section geometry is a 1.07 cm diameter heater rod within a 4.29 diameter flow channel

  20. Molten Chloride Salts for Heat Transfer in Nuclear Systems

    Science.gov (United States)

    Ambrosek, James Wallace

    2011-12-01

    A forced convection loop was designed and constructed to examine the thermal-hydraulic performance of molten KCl-MgCl2 (68-32 at %) salt for use in nuclear co-generation facilities. As part of this research, methods for prediction of the thermo-physical properties of salt mixtures for selection of the coolant salt were studied. In addition, corrosion studies of 10 different alloys were exposed to the KCl-MgCl2 to determine a suitable construction material for the loop. Using experimental data found in literature for unary and binary salt systems, models were found, or developed to extrapolate the available experimental data to unstudied salt systems. These property models were then used to investigate the thermo-physical properties of the LINO3-NaNO3-KNO 3-Ca(NO3), system used in solar energy applications. Using these models, the density, viscosity, adiabatic compressibility, thermal conductivity, heat capacity, and melting temperatures of higher order systems can be approximated. These models may be applied to other molten salt systems. Coupons of 10 different alloys were exposed to the chloride salt for 100 hours at 850°C was undertaken to help determine with which alloy to construct the loop. Of the alloys exposed, Haynes 230 had the least amount of weight loss per area. Nickel and Hastelloy N performed best based on maximum depth of attack. Inconel 625 and 718 had a nearly uniform depletion of Cr from the surface of the sample. All other alloys tested had depletion of Cr along the grain boundaries. The Nb in Inconel 625 and 718 changed the way the Cr is depleted in these alloys. Grain-boundary engineering (GBE) of Incoloy 800H improved the corrosion resistance (weight loss and maximum depth of attack) by nearly 50% as compared to the as-received Incoloy 800H sample. A high temperature pump, thermal flow meter, and pressure differential device was designed, constructed and tested for use in the loop, The heat transfer of the molten chloride salt was found to

  1. Human somatic cell nuclear transfer and reproductive cloning: an Ethics Committee opinion.

    Science.gov (United States)

    2016-04-01

    This document presents arguments that conclude that it is unethical to use somatic cell nuclear transfer (SCNT) for infertility treatment due to concerns about safety; the unknown impact of SCNT on children, families, and society; and the availability of other ethically acceptable means of assisted reproduction. This document replaces the ASRM Ethics Committee report titled, "Human somatic cell nuclear transfer and cloning," last published in Fertil Steril 2012;98:804-7.

  2. Human somatic cell nuclear transfer and reproductive cloning: an Ethics Committee opinion.

    Science.gov (United States)

    2016-04-01

    This document presents arguments that conclude that it is unethical to use somatic cell nuclear transfer (SCNT) for infertility treatment due to concerns about safety; the unknown impact of SCNT on children, families, and society; and the availability of other ethically acceptable means of assisted reproduction. This document replaces the ASRM Ethics Committee report titled, "Human somatic cell nuclear transfer and cloning," last published in Fertil Steril 2012;98:804-7. PMID:26746137

  3. Government/industry development of a spent nuclear fuel dry transfer system

    International Nuclear Information System (INIS)

    The United States Department of Energy (DOE) is currently engaged in a cooperative program with the Electric Power Research Institute (EPRI) to develop a spent nuclear fuel dry transfer system (DTS). The system will enable the transfer of individual spent nuclear fuel assemblies between a conventional top loading cask and multi-purpose canister in a shielded overpack, or accommodate spent nuclear fuel transfers between two conventional casks. The DTS has several significant applications and could benefit the Federal waste management system and utilities in number of ways. It has the potential to: permit shutdown reactor sites to decommission pools; provide capability at interim storage facilities to transfer assemblies from small transportation casks to sealed canisters; provide capability at reactor sites with limited crane capacity to transfer assemblies into large packages to facilitate on-site storage in larger capacity casks; allow recovery operations at shutdown reactor sites with independent spent nuclear fuel storage installations; provide a means for utilities that can presently handle only a truck cask to utilize a rail cask; allow transfers of spent nuclear fuel from existing utility dual purpose systems into alternative systems if required, without returning to the reactor storage pool; and support existing and future DOE spent nuclear fuel management activities. The project is managed by a Technical Management Committee consisting of DOE and EPRI representatives and includes a member from Battelle Pacific Northwest National Laboratory. (authors)

  4. Comparison of passive transfer of immunity in neonatal dairy calves fed colostrum or bovine serum-based colostrum replacement and colostrum supplement products

    Science.gov (United States)

    Poulsen, Keith P.; Foley, Andrea L.; Collins, Michael T.; McGuirk, Sheila M.

    2011-01-01

    Objective To compare serum total protein (sTP) and serum IgG (sIgG) concentrations in neonatal calves administered colostrum or a bovine serum-based colostrum replacement (CR) product followed by a bovine serum-based colostrum supplement (CS) product. Design Randomized controlled clinical trial. Animals 18 Jersey and 269 Holstein neonatal heifer calves. Procedures 141 calves were given 4 L of colostrum in 1 or 2 feedings (first or only feeding was provided ≤ 2 hours after birth; when applicable, a second feeding was provided between 2 and 12 hours after birth). Other calves (n = 146) were fed 2 L of a CR product ≤ 2 hours after birth and then 2 L of a CS product between 2 and 12 hours after birth. Concentrations of sTP and sIgG were measured 1 to 7 days after birth. Data from cohorts on individual farms and for all farms were analyzed. Results Mean sTP and sIgG concentrations differed significantly between feeding groups. In calves fed colostrum and calves fed CR and CS products, mean ± SD sTP concentration was 5.58 ± 0.67 g/dL and 5.26 ± 0.54 g/dL, respectively, and mean sIgG concentration was 1,868 ± 854 mg/dL and 1,320 ± 620 mg/dL, respectively. The percentage of calves that had failure of passive transfer of immunity (ie, sIgG concentrations < 1,000 mg/dL) was not significantly different between groups. Conclusions and Clinical Relevance Results suggested that sequential feeding of bovine serum-based CR and CS products to neonatal calves is an alternative to feeding colostrum for achieving passive transfer of immunity. PMID:20946083

  5. Technology transfer of nuclear power development in developing countries: Case study of China

    International Nuclear Information System (INIS)

    This paper describes the specific experiences in the technology transfer of nuclear power in China, a country that both imported and developed indigenous nuclear technology. Based on this experience some recommendations are presented that should be considered particularly by the developing countries. (author)

  6. Heat transfer burnout in tube-type fuel elements of nuclear power reactors

    International Nuclear Information System (INIS)

    The conditions are formulated under which the results of the experimental research of the boilino. water heat transfer burnout carried out on models may be applied to fuel elements of nuclear reactors. Experimental material providing data on the heat transfer burnout was expanded by the results of measurements of the uneven (cosine) longitudinal distribution of heat sources. The results of the effects of helical fins or wires on heat transfer burnout are presented. (F.M.)

  7. Technical knowledge/skill transfer in nuclear division of Hitachi group

    International Nuclear Information System (INIS)

    Due to environmental concerns such as global warming, needs the nuclear power is increasing. However, many expert engineers and technicians are now entering a period of retirement. And due to weak demands of new plant construction for long years, opportunity for technology learning/experience had been lost. Therefore, to secure human resource and to develop their ability are urgent issues for nuclear industries. Hitachi nuclear division continues efforts for technology transfer and human resource training. This paper describes the following two activities. (1) Improvement of common technical basis, and implementation of PDCA cycle. (2) Development of supporting tools to accelerate technology transfer through OJT (On the Job Training). (author)

  8. Generation of cloned mice and nuclear transfer embryonic stem cell lines from urine-derived cells.

    Science.gov (United States)

    Mizutani, Eiji; Torikai, Kohei; Wakayama, Sayaka; Nagatomo, Hiroaki; Ohinata, Yasuhide; Kishigami, Satoshi; Wakayama, Teruhiko

    2016-01-01

    Cloning animals by nuclear transfer provides the opportunity to preserve endangered mammalian species. However, there are risks associated with the collection of donor cells from the body such as accidental injury to or death of the animal. Here, we report the production of cloned mice from urine-derived cells collected noninvasively. Most of the urine-derived cells survived and were available as donors for nuclear transfer without any pretreatment. After nuclear transfer, 38-77% of the reconstructed embryos developed to the morula/blastocyst, in which the cell numbers in the inner cell mass and trophectoderm were similar to those of controls. Male and female cloned mice were delivered from cloned embryos transferred to recipient females, and these cloned animals grew to adulthood and delivered pups naturally when mated with each other. The results suggest that these cloned mice had normal fertility. In additional experiments, 26 nuclear transfer embryonic stem cell lines were established from 108 cloned blastocysts derived from four mouse strains including inbreds and F1 hybrids with relatively high success rates. Thus, cells derived from urine, which can be collected noninvasively, may be used in the rescue of endangered mammalian species by using nuclear transfer without causing injury to the animal. PMID:27033801

  9. Nuclear transfer of goat somatic cells transgenic for human lactoferrin gene

    Institute of Scientific and Technical Information of China (English)

    Lan LI; Wei SHEN; Lingjiang MIN; Qingyu PAN; Yujiang SUN; Jixian DENG; Qingjie PAN

    2008-01-01

    Transgenic animal mammary gland bioreactors are used to produce recombinant proteins with appropri-ate post-translational modifications.The nuclear transfer of transgenic somatic cells is a powerful method to pro-duce mammary gland bioreactors.We established an effi-cient gene transfer and nuclear transfer approach in goat somatic cells.Gene targeting vector pGBC2LF was con-structed by cloning human lactoferrin (LF) gene cDNA into exon 2 of the milk goat beta-casein gene and the endogenous start codon was replaced by that of human LF gene.Goat fetal fibroblasts were transfected with lin-earized pGBC2LF and 14 cell lines were positive accord-ing to PCR and Southern blot.The transgenic cells were used as donor cells of nuclear transfer and some of recon-structed embryos could develop into blastocyst in vitro.

  10. The United States experience in the transfer of nuclear tecnology

    International Nuclear Information System (INIS)

    The paper surveys U.S. public policies and program for nuclear energy development. In the U.S., nuclear technology has been applied for public benefit. Today it is being relied on to meet electrical power requirements. Radioisotope technology is being applied in industry, agriculture and medicine. In 1947 when AEC was established, nuclear technologies moved from research and development phase to useful products and processes. After energy shortages in 1974, a new agency (ERDA) was created, which brought together research and development programs in solar, geothermal and fossil, as well as energy systems. The Atomic Energy Act was amended in 1954 to permit cooperation with foreign countries. Domestically, the AEC engaged in a series of Power Reactor Demonstration with U.S. industry that in a decade led to the demonstration of proven and economic light-water reactors. In 1957 IAEA was established, and then the whole series of AEC policy changed by a strong information dissemination program. ERDA unified the Government's effort to provide a variety of energy choices in nuclear and non-nuclear areas

  11. 78 FR 11904 - Zion Nuclear Power Station, Units 1 and 2; ZionSolutions, LLC; Consideration of Indirect Transfer

    Science.gov (United States)

    2013-02-20

    ... From the Federal Register Online via the Government Publishing Office NUCLEAR REGULATORY COMMISSION Zion Nuclear Power Station, Units 1 and 2; ZionSolutions, LLC; Consideration of Indirect Transfer... the indirect transfer of Facility Operating License Nos. DPR-39 and DPR-48 for Zion Nuclear...

  12. Momentum transfer dependence of the quasielastic nuclear response in (e,e')

    International Nuclear Information System (INIS)

    Recent 56Fe(e,e') datal at momentum transfer |q| = 1.14 GeV displays behavior which is qualitatively different from that of lower momentum transfers where relativistic mean field theory provides a semiquantitative understanding. An explanation of this difference is offered based on an analysis of the longitudinal response in nuclear matter. An ansatz is made for the self-energy functions in the nuclear medium which suppresses medium effects for momenta above the nucleon mass scale. This suppression is shown in a local density calculation to give consistent qualitative agreement with both the low and high momentum transfer data, although the experimental longitudinal response is still systematically below the calculation at high energy transfers. The calculation provides motivation for further experimental investigations in the momentum transfer region between 0.5 and 1.0 GeV

  13. Nuclear EMP: stripline test method for measuring transfer impedance

    International Nuclear Information System (INIS)

    A method for measuring the transfer impedance of flat metal joints for frequencies to 100 MHz has been developed which makes use of striplines. The stripline method, which has similarities to the quadraxial method used for cylindrical components, is described and sets of test results are given. The transfer impedance of a simple joint is modeled as a spurious hyperbolic curve, and a close curve fit to transfer impedance test data from various samples is demonstrated for both the stripline and the quadraxial methods. Validity checks of the test data are discussed using the curve model and other criteria. The method was developed for testing riveted joints which form the avionics bays on B-1s. The joints must provide shielding from EMP currents

  14. Thermoacoustic sensor for nuclear fuel temperaturemonitoring and heat transfer enhancement

    Energy Technology Data Exchange (ETDEWEB)

    James A. Smith; Dale K. Kotter; Randall A. Alli; Steven L. Garrett

    2013-05-01

    A new acoustical sensing system for the nuclear power industry has been developed at The Pennsylvania State University in collaboration with Idaho National Laboratories. This sensor uses the high temperatures of nuclear fuel to convert a nuclear fuel rod into a standing-wave thermoacoustic engine. When a standing wave is generated, the sound wave within the fuel rod will be propagated, by acoustic radiation, through the cooling fluid within the reactor or spent fuel pool and can be monitored a remote location external to the reactor. The frequency of the sound can be correlated to an effective temperature of either the fuel or the surrounding coolant. We will present results for a thermoacoustic resonator built into a Nitonic-60 (stainless steel) fuel rod that requires only one passive component and no heat exchangers.

  15. Westinghouse experience in the transfer of nuclear technology

    International Nuclear Information System (INIS)

    Westinghouse experience with transfer of technical information is two-sided. First is our experience in learning, and the second is our experience in teaching others. Westinghouse conducts a special school to which government, academic and industry people are invited. There are many problems involved in all technology transfers; these include: keeping information current, making certain changes are compatible with the supplier's manufacturing capability and also suitable to the receiver, patent right and proprietary information. The building, testing and maintenance of the unit on the line - and then a succession of its sister plant is the basis for the Westinghouse leadership

  16. Squids, supercurrents, and slope anomalies: Nuclear structure from heavy-ion transfer reactions

    International Nuclear Information System (INIS)

    Within the past five years we have developed experimental techniques to study heavy-ion transfer reactions to high spin states in deformed nuclei. These methods have been turned into a quantitative tool to assess the influence of collective excitation on single-particle and pairing structure. I discuss some of the nuclear structure questions which are being answered in these experiments: How strong is ground state pairing? How does pairing change with angular momentum? Why is two-neutron transfer much stronger than expected at large radial separation? What is the evidence for a nuclear Josephson Effect? What is the evidence for a nuclear Berry phase effect (nuclear SQUID)? Why does one-neutron transfer populate much higher spins than would be naively expected? Conversely, why does two-neutron transfer populate much lower spins than anyone expected? The answer to each of these questions involves the influence of detailed nuclear structure on transfer reactions, and represents quantitative new information about the effect of angular momentum and excitation energy on many-body systems with a finite number of particles. 8 refs., 6 figs

  17. Functional full-term placentas formed from parthenogenetic embryos using serial nuclear transfer.

    Science.gov (United States)

    Hikichi, Takafusa; Ohta, Hiroshi; Wakayama, Sayaka; Wakayama, Teruhiko

    2010-09-01

    Mammalian parthenogenetic embryos invariably die in mid-gestation from imprinted gene defects and placental hypoplasia. Based on chimera experiments, trophoblastic proliferation is supposed to be inhibited in the absence of a male genome. Here, we show that parthenogenetic mouse embryonic cell nuclei can be reprogrammed by serial rounds of nuclear transfer without using any genetic modification. The durations of survival in uteri of cloned foetuses derived from green fluorescent protein (GFP)-labelled parthenogenetic cell nuclei were extended with repeated nuclear transfers. After five repeats, live cloned foetuses were obtained up to day 14.5 of gestation; however, they did not survive longer even when we repeated nuclear transfer up to nine times. All foetuses showed intestinal herniation and possessed well-expanded large placentas. When embryonic stem (ES) cells derived from fertilised embryos were aggregated with the cloned embryos, full-term offspring with large placentas were obtained from the chimeric embryos. Those placentas were derived from parthenogenetic cell nuclei, judging from GFP expression. The patterns of imprinted gene expression and methylation status were similar to their parthenogenetic origin, except for Peg10, which showed the same level as in the normal placenta. These results suggest that there is a limitation for foetal development in the ability to reprogramme imprinted genes by repeated rounds of nuclear transfer. However, the placentas of parthenogenetic embryos can escape epigenetic regulation when developed using nuclear transfer techniques and can support foetal development to full gestation.

  18. Modeling transient heat transfer in nuclear waste repositories.

    Science.gov (United States)

    Yang, Shaw-Yang; Yeh, Hund-Der

    2009-09-30

    The heat of high-level nuclear waste may be generated and released from a canister at final disposal sites. The waste heat may affect the engineering properties of waste canisters, buffers, and backfill material in the emplacement tunnel and the host rock. This study addresses the problem of the heat generated from the waste canister and analyzes the heat distribution between the buffer and the host rock, which is considered as a radial two-layer heat flux problem. A conceptual model is first constructed for the heat conduction in a nuclear waste repository and then mathematical equations are formulated for modeling heat flow distribution at repository sites. The Laplace transforms are employed to develop a solution for the temperature distributions in the buffer and the host rock in the Laplace domain, which is numerically inverted to the time-domain solution using the modified Crump method. The transient temperature distributions for both the single- and multi-borehole cases are simulated in the hypothetical geological repositories of nuclear waste. The results show that the temperature distributions in the thermal field are significantly affected by the decay heat of the waste canister, the thermal properties of the buffer and the host rock, the disposal spacing, and the thickness of the host rock at a nuclear waste repository. PMID:19376651

  19. Fatal attraction: Explaining Russia's sensitive nuclear transfers to Iran

    Science.gov (United States)

    Kuchinsky, Leah R.

    This paper explores Russia's sensitive nuclear assistance to Iran in an effort to determine why a supplier state might proliferate against its own apparent security interests. The goal is to help readers understand the supply-side dynamics of nuclear proliferation. Through careful reconstruction of the historical narrative, using open source data, this study tests the plausibility of a "fatalistic calculus" explanation, identified by Stephen Sestanovich as a possible driver for Russia's behavior. According to the hypothesis, Russia has cooperated with Iran as a way both to stay in the good graces of a neighbor that is suspected of developing nuclear weapons and to win short-term influence and profits. The paper also examines the role of other factors advanced in the existing supply-side literature, such as economic motives identified by physicist and nonproliferation scholar David Albright. The findings show that bureaucratic, economic and fatalistic factors have each played a role in motivating Russia's cooperation with Iran, with their relative importance shifting over time. Fatalism begets a strategy of Russian "minimaxing," in the lexicon of Russia scholar Robert Freedman, wherein Russia attempts to minimize damage to its relationship with the U.S. while maximizing influence in Iran via nuclear cooperation. Fatalism, as actualized by minimaxing, best explains Russia's behavior after former Russian president Vladmir Putin came to power, when the bureaucratic and economic arguments become less cogent.

  20. Modeling transient heat transfer in nuclear waste repositories.

    Science.gov (United States)

    Yang, Shaw-Yang; Yeh, Hund-Der

    2009-09-30

    The heat of high-level nuclear waste may be generated and released from a canister at final disposal sites. The waste heat may affect the engineering properties of waste canisters, buffers, and backfill material in the emplacement tunnel and the host rock. This study addresses the problem of the heat generated from the waste canister and analyzes the heat distribution between the buffer and the host rock, which is considered as a radial two-layer heat flux problem. A conceptual model is first constructed for the heat conduction in a nuclear waste repository and then mathematical equations are formulated for modeling heat flow distribution at repository sites. The Laplace transforms are employed to develop a solution for the temperature distributions in the buffer and the host rock in the Laplace domain, which is numerically inverted to the time-domain solution using the modified Crump method. The transient temperature distributions for both the single- and multi-borehole cases are simulated in the hypothetical geological repositories of nuclear waste. The results show that the temperature distributions in the thermal field are significantly affected by the decay heat of the waste canister, the thermal properties of the buffer and the host rock, the disposal spacing, and the thickness of the host rock at a nuclear waste repository.

  1. Pig transgenesis by piggyBac transposition in combination with somatic cell nuclear transfer.

    Science.gov (United States)

    Wu, Zhenfang; Xu, Zhiqian; Zou, Xian; Zeng, Fang; Shi, Junsong; Liu, Dewu; Urschitz, Johann; Moisyadi, Stefan; Li, Zicong

    2013-12-01

    The production of animals by somatic cell nuclear transfer (SCNT) is inefficient, with approximately 2% of micromanipulated oocytes going to term and resulting in live births. However, it is the most commonly used method for the generation of cloned transgenic livestock as it facilitates the attainment of transgenic animals once the nuclear donor cells are stably transfected and more importantly as alternatives methods of transgenesis in farm animals have proven even less efficient. Here we describe piggyBac-mediated transposition of a transgene into porcine primary cells and use of these genetically modified cells as nuclear donors for the generation of transgenic pigs by SCNT. Gene transfer by piggyBac transposition serves to provide an alternative approach for the transfection of nuclear donor cells used in SCNT.

  2. Treating humanity as an inviolable end: an analysis of contraception and altered nuclear transfer.

    Science.gov (United States)

    Masek, Lawrence

    2008-04-01

    I argue that contraception is morally wrong but that periodic abstinence (or natural family planning) is not. Further, I argue that altered nuclear transfer -- a proposed technique for creating human stem cells without destroying human embryos -- is morally wrong for the same reason that contraception is. Contrary to what readers might expect, my argument assumes nothing about the morality of cloning or abortion and requires no premises about God or natural teleology. Instead, I argue that contraception and altered nuclear transfer are morally wrong because they fail to treat humanity as an inviolable end. PMID:18480499

  3. Experimental cloning of embryos through human-rabbit inter-species nuclear transfer

    Institute of Scientific and Technical Information of China (English)

    JI Jingjuan; GUO Tonghang; TONG Xianhong; LUO Lihua; ZHOU Guixiang; FU Yingyun; LIU Yusheng

    2007-01-01

    Therapeutic cloning,which is based on human somatic cell nuclear transfer,is one of our major research objectives.Though inter-species nuclear transfer has been introduced to construct human somatic cell cloned embryos,the effects of type,passage,and preparation method of donor cells on embryo development remain unclear.In our experiment,cloned embryos were reconstructed with different passage and preparation methods of ossocartilaginous cell,skin fibroblast,and cumulus cells.The cumulus cell embryos showed significantly higher development rates than the other two (P<0.05).The development rate of embryos reconstructed with skin fibroblasts of different passage number and somatic cells of different chilling durations showed no significant difference.Also,fluorescence in situ hybridization (FISH)was conducted to detect nuclear derivation of the embryos.The result showed that the nuclei of the inter-species cloned embryo cells came from human.We conclude that (1)cloned embryos can be constructed through human-rabbit interspecies nuclear transfer;(2)different kinds of somatic cells result in different efficiency of nuclear transfer,while in vitro passage of the donor does not influence embryo development;(3)refrigeration is a convenient and efficient donor cell preparation method.Finally,it is feasible to detect DNA gcnotype through FISH.

  4. Cell-mediated transgenesis in rabbits: chimeric and nuclear transfer animals.

    Science.gov (United States)

    Zakhartchenko, V; Flisikowska, T; Li, S; Richter, T; Wieland, H; Durkovic, M; Rottmann, O; Kessler, B; Gungor, T; Brem, G; Kind, A; Wolf, E; Schnieke, A

    2011-02-01

    The ability to perform precise genetic engineering such as gene targeting in rabbits would benefit biomedical research by enabling, for example, the generation of genetically defined rabbit models of human diseases. This has so far not been possible because of the lack of functional rabbit embryonic stem cells and the high fetal and perinatal mortality associated with rabbit somatic cell nuclear transfer. We examined cultured pluripotent and multipotent cells for their ability to support the production of viable animals. Rabbit putative embryonic stem (ES) cells were derived and shown capable of in vitro and in vivo pluripotent differentiation. We report the first live born ES-derived rabbit chimera. Rabbit mesenchymal stem cells (MSCs) were derived from bone marrow, and multipotent differentiation was demonstrated in vitro. Nuclear transfer was carried out with both cell types, and embryo development was assessed in vitro and in vivo. Rabbit MSCs were markedly more successful than ES cells as nuclear donors. MSCs were transfected with fluorescent reporter gene constructs and assessed for nuclear transfer competence. Transfected MSCs supported development with similar efficiency as normal MSCs and resulted in the first live cloned rabbits from genetically manipulated MSCs. Reactivation of fluorescence reporter gene expression in reconstructed embryos was investigated as a means of identifying viable embryos in vitro but was not a reliable predictor. We also examined serial nuclear transfer as a means of rescuing dead animals.

  5. High fidelity transfer and storage of photon states in a single nuclear spin

    OpenAIRE

    Yang, Sen; Wang, Ya; Rao, D. D. Bhaktavatsala; Tran, Thai Hien; Momenzadeh, S. Ali; Nagy, Roland; Markham, M.; Twitchen, D. J.; Ping WANG; Yang, Wen; Stoehr, Rainer; Neumann, Philipp; Kosaka, Hideo; Wrachtrup, Joerg

    2015-01-01

    Building a quantum repeater network for long distance quantum communication requires photons and quantum registers that comprise qubits for interaction with light, good memory capabilities and processing qubits for storage and manipulation of photons. Here we demonstrate a key step, the coherent transfer of a photon in a single solid-state nuclear spin qubit with an average fidelity of 98% and storage over 10 seconds. The storage process is achieved by coherently transferring a photon to an e...

  6. From nuclear stagnation to renaissance: The challenge of transferring regulatory knowledge

    International Nuclear Information System (INIS)

    Full text: Around 20 years ago there was a de facto nuclear stagnation of the peaceful uses of atomic energy in the West. At the time, governmental institutions for nuclear research and regulation, as well as the nuclear industry, had a nuclear 'intelligentsia' and many well qualified specialists available. Moreover, universities were ready and able to educate the next generation of professionals. The decline of the nuclear industry was therefore damaging both for individuals and the schools educating them. The options were few: for the workers, to restart a working life in other industries or to try to hang on until retirement; for the educational institutions, to abandon researching and teaching nuclear subjects. Now, however, an emerging energy crisis and growing environmental concerns about the burning of fossil fuels are inducing Western politicians to rethink the soundness of the decision to curtail the nuclear power industry. It therefore seems that there will be a nuclear renaissance, but that the qualified personnel to carry this out are no longer available, since in the intervening years they have changed jobs, retired or died. Put simply, the plentiful workforce responsible for last century's nuclear miracle is no longer there. Moreover, a cultural gap exists between the past and present generations, and such a lack of cultural continuity creates a difficult challenge for the traditional inter-generational knowledge transfer that has enabled development in the past. There are many reasons for this situation, and it will be for historians to explain them, but it is obvious that the Chernobyl accident and the public belief that nuclear installations were not safely regulated played a crucial role. At the time, nuclear regulatory authorities were less demanding, and in many countries they simply did not exist. Concepts such as regulatory independence and strengthening and safety culture are generally post-Chernobyl phenomena. Moreover, over the past decades

  7. Order of 28 March 1980 on the transfer to ENUSA of duties of the Junta de Energia Nuclear connected with the nuclear fuel cycle

    International Nuclear Information System (INIS)

    In implementation of the Royal Decree of 7 December 1979 the Minister of Industry and Energy made this Order regulating the transfer to ENUSA (National Uranium Undertaking) of the Junta de Energia Nuclear's duties relating to the nuclear fuel cycle. The Order sets up, within the Ministry of Industry and Energy, a Transfer Commission responsible for establishing the directives prior to the measures to be taken by the Ministry concerning the transfer to ENUSA of the duties, personnel and establishments of the Junta connected with the nuclear fuel cycle. It will also determine the dates of such transfer, according to the order of priority laid down in the Order. (NEA)

  8. Quantum state transfer between an optomechanical cavity and a diamond nuclear spin ensemble

    Science.gov (United States)

    Feng, Zhi-Bo; Wang, Hong-Ling; Yan, Run-Ying

    2016-08-01

    We explore an efficient scheme for transferring quantum state between an optomechanical cavity and nuclear spins of nitrogen-vacancy centers in diamond, where quantum information can be efficiently stored (retrieved) into (from) the nuclear spin ensemble assisted by a mechanical resonator in a dispersive regime. Our scheme works for a broad range of cavity frequencies and might have potential applications in employing the nuclear spin ensemble as a memory in quantum information processing. The feasibility of our protocol is analyzed using currently available parameters.

  9. Passive heat-transfer means for nuclear reactors. [LMFBR

    Science.gov (United States)

    Burelbach, J.P.

    1982-06-10

    An improved passive cooling arrangement is disclosed for maintaining adjacent or related components of a nuclear reactor within specified temperature differences. Specifically, heat pipes are operatively interposed between the components, with the vaporizing section of the heat pipe proximate the hot component operable to cool it and the primary condensing section of the heat pipe proximate the other and cooler component operable to heat it. Each heat pipe further has a secondary condensing section that is located outwardly beyond the reactor confinement and in a secondary heat sink, such as air ambient the containment, that is cooler than the other reactor component. By having many such heat pipes, an emergency passive cooling system is defined that is operative without electrical power.

  10. Transference of know-how for the fabrication of heavy components for nuclear power reactors

    International Nuclear Information System (INIS)

    1) Heavy components for nuclear power reactors. Reactor pressure vessels with total weight of 540 tons; steam generators: heat exchangers with U-type tube bundles, total weight 420 tons. 2) Choice of know-how recipient. Technical criteria, i.e. manufacturing facilities, existing quality assurance system, location of the workshops, possibilities for training, infrastructures. 3. Measures for transferring know-how to a newly established company. Planning and erection of the factory: organisational set up of the company; personnel selection and training; transfer of documentation; transfer of know-how that cannot be transferred in a written form. 4) Contracts for assuring the transfer of know-how. Stipulation of mutual rights and obligations of the know-how owner and receiver in individual contracts: engineering services contract, technical information contract, personnel training contract, license contract. (orig.)

  11. One-two step transfer observed in 16O + 11B nuclear system

    International Nuclear Information System (INIS)

    The angular distribution measurements for 16O ion beam elastically scattered from 11B target of thickness 32.9μg/cm2 at energy 22.4 MeV had been performed in the cyclotron DC-60 INP NNC RK. The previous measurements for 16O + 11B nuclear system at energies 27, 30, 32.5 and 35 MeV showed an increase in the differential cross-section at backward angles due to the contribution of cluster transfer. Such transfer process could not be described in terms of optical model (OM); it could be described within the framework of distorted wave Born approximation method implemented in FRESCO code. Both one (5Li) and two-step transfer (proton transfer followed by Alpha transfer) were taken into considerations. We have extracted the spectroscopic amplitude (SA) for the configuration 16O → 11B + 5Li. (author)

  12. Coherent nuclear wave packet dynamics of laurdan launched by intramolecular charge transfer

    Directory of Open Access Journals (Sweden)

    Kim S. Y.

    2013-03-01

    Full Text Available Coherent nuclear wave packets in the product state launched by the ultrafast intramolecular charge transfer are observed by time-resolved fluorescence with 40 fs time resolution. Direct information on reaction coordinates and structural changes can be obtained.

  13. 78 FR 67925 - Transfer of Real Property at Defense Nuclear Facilities for Economic Development

    Science.gov (United States)

    2013-11-13

    ... process it will follow in the development of such regulations (65 FR 13735). DOE has examined today's rule...-AA82 Transfer of Real Property at Defense Nuclear Facilities for Economic Development AGENCY... final rule published on February 29, 2000, 65 FR 10685, as final, with changes. The final...

  14. Nuclear analysis software. Pt. 1: Spectrum transfer and reformatting (SPEDAC)

    International Nuclear Information System (INIS)

    GANAAS (Gamma, Activity, and Neutron Activation Analysis System) is one in the family of software packages developed under the auspices of the International Atomic Energy Agency. Primarily, the package was intended to support the IAEA Technical Assistance and Cooperation projects in developing countries. However, it is open domain software that can be copied and used by anybody, except for commercial purposes. All the nuclear analysis software provided by the IAEA has the same design philosophy and similar structure. The intention was to provide the user with maximum flexibility, at the same time with a simple and logical organization that requires minimum digging through the manuals. GANAAS is a modular system. It consists of several programmes that can be installed on the hard disk as the are needed. Obviously, some parts of they system are required in all cases. Those are installed at the beginning, without consulting the operator. GANAAS offers the opportunity to expand and improve the system. The gamma spectrum evaluation programmes using different fitting algorithms can be added to GANAAS, under the condition that the format of their input and output files corresponds to the rules of GANAAS. The same applies to the quantitative analysis parts of the programme

  15. I型牛疱疹病毒通用型转移载体的构建%Construction of a Universal Bovine Herpesvirus I Transfer Vector

    Institute of Scientific and Technical Information of China (English)

    李继昌; 童光志; 仇华吉; 周艳君; 张桂红; 王柳; 刘忠贵

    2001-01-01

    将I型牛疱疹病毒(BHV-1)LA株DNA HindIII A片段中的SalI-SalI亚片段(含TK基因)克隆到载体质粒pBluescript SK中,再用BglII和SacI切去347 bp,获得含TK基因部分缺失的重组质粒pSdTK,然后用HindIII和XbaI切去其中的多克隆位点;将来源于pCR3-Uni的CMV启动子、多克隆位点和BGH polyA信号插入pSdTK的XhoI位点上,构建了BHV-1通用转移载体pSdTK-CMB,此载体可用来表达牛其它病毒的抗原基因,为开发二价或多价基因工程疫苗提供基础。%Bovine herpes irus-1 strain LA DNA HindIII A was digested by the restriction endonuclease SalI,The SalI subfragment of 2.7kb containing thymidine kinase(TK)gene was cloned into pBluescript SK,resulting in a recombinant pSTK,Then it was digested by BglII and SacI,the 5.31kb fragment was recovered and self-ligated,resulting in a recombinant pSdTK,the multiple colning sites were removed by HindIII and XbaI;A fragment containing the immediate early promoter of cytomegalovirus,multiple cloning sites and bovine growth hormone polyadenylation signal derived from pCR3-Uni was amplified and inserted into the XhoI site of pSdTK.The resulting transfer vector pSdTK-CMB can be used to expess genes from other bovine viruses(i.e. BPIV3、BRSV、BVDV).

  16. High-fidelity transfer and storage of photon states in a single nuclear spin

    Science.gov (United States)

    Yang, Sen; Wang, Ya; Rao, D. D. Bhaktavatsala; Hien Tran, Thai; Momenzadeh, Ali S.; Markham, M.; Twitchen, D. J.; Wang, Ping; Yang, Wen; Stöhr, Rainer; Neumann, Philipp; Kosaka, Hideo; Wrachtrup, Jörg

    2016-08-01

    Long-distance quantum communication requires photons and quantum nodes that comprise qubits for interaction with light and good memory capabilities, as well as processing qubits for the storage and manipulation of photons. Owing to the unavoidable photon losses, robust quantum communication over lossy transmission channels requires quantum repeater networks. A necessary and highly demanding prerequisite for these networks is the existence of quantum memories with long coherence times to reliably store the incident photon states. Here we demonstrate the high-fidelity (˜98%) coherent transfer of a photon polarization state to a single solid-state nuclear spin that has a coherence time of over 10 s. The storage process is achieved by coherently transferring the polarization state of a photon to an entangled electron-nuclear spin state of a nitrogen-vacancy centre in diamond. The nuclear spin-based optical quantum memory demonstrated here paves the way towards an absorption-based quantum repeater network.

  17. The onset of foreign gene transcription in nuclear-transferred embryos of fish

    Institute of Scientific and Technical Information of China (English)

    孙永华; 陈尚萍; 汪亚平; 朱作言

    2000-01-01

    The transcriptional onset ot hGH-transgene in fish was studied in the following three cases: the first is in MThGH-transgenic F4 common carp (Cyprinus carpio) embryos, the second is in nuclear-transferred embryos supported by the transgenic F4 embryonic nuclei, and the third is in nuclear-transferred embryos supported by the transgenic F4 tail-fin nuclei. RT-PCR results show that the hGH-transgene initiates its transcriptional activity from early-gastrula stage, the early blastula stage and even 16-cell stage in the first, second and third cases, respectively. It looks like that fish egg cytoplasm could just offer a very restricted reprogramming on transcriptional activity of specific gene in differentiated cell nuclei by nuclear transplantation.

  18. The onset of foreign gene transcription in nuclear-transferred embryos of fish

    Institute of Scientific and Technical Information of China (English)

    2000-01-01

    The transcriptional onset of hGH-transgene in fish was studied in the following three cases: the first is in MThGH-transgenic F4 common carp (Cyprinus carpio) embryos, the second is in nuclear-transferred embryos supported by the transgenic F4 embryonic nuclei, and the third is in nuclear-transferred embryos supported by the transgenic F4 tail-fin nuclei. RT-PCR results show that the hGH-transgene initiates its transcriptional activity from early-gastrula stage, the early blas-tula stage and even 16-cell stage in the first, second and third cases, respectively. It looks like that fish egg cytoplasm could just offer a very restricted reprogramming on transcriptional activity of specific gene in differentiated cell nuclei by nuclear transplantation.

  19. Modularization and nuclear power. Report by the Technology Transfer Modularization Task Team

    Energy Technology Data Exchange (ETDEWEB)

    1985-06-01

    This report describes the results of the work performed by the Technology Transfer Task Team on Modularization. This work was performed as part of the Technology Transfer work being performed under Department of Energy Contract 54-7WM-335406, between December, 1984 and February, 1985. The purpose of this task team effort was to briefly survey the current use of modularization in the nuclear and non-nuclear industries and to assess and evaluate the techniques available for potential application to nuclear power. A key conclusion of the evaluation was that there was a need for a study to establish guidelines for the future development of Light Water Reactor, High Temperature Gas Reactor and Liquid Metal Reactor plants. The guidelines should identify how modularization can improve construction, maintenance, life extension and decommissioning.

  20. High-fidelity transfer and storage of photon states in a single nuclear spin

    Science.gov (United States)

    Yang, Sen; Wang, Ya; Rao, D. D. Bhaktavatsala; Hien Tran, Thai; Momenzadeh, Ali S.; Markham, M.; Twitchen, D. J.; Wang, Ping; Yang, Wen; Stöhr, Rainer; Neumann, Philipp; Kosaka, Hideo; Wrachtrup, Jörg

    2016-08-01

    Long-distance quantum communication requires photons and quantum nodes that comprise qubits for interaction with light and good memory capabilities, as well as processing qubits for the storage and manipulation of photons. Owing to the unavoidable photon losses, robust quantum communication over lossy transmission channels requires quantum repeater networks. A necessary and highly demanding prerequisite for these networks is the existence of quantum memories with long coherence times to reliably store the incident photon states. Here we demonstrate the high-fidelity (∼98%) coherent transfer of a photon polarization state to a single solid-state nuclear spin that has a coherence time of over 10 s. The storage process is achieved by coherently transferring the polarization state of a photon to an entangled electron–nuclear spin state of a nitrogen–vacancy centre in diamond. The nuclear spin-based optical quantum memory demonstrated here paves the way towards an absorption-based quantum repeater network.

  1. Modularization and nuclear power. Report by the Technology Transfer Modularization Task Team

    International Nuclear Information System (INIS)

    This report describes the results of the work performed by the Technology Transfer Task Team on Modularization. This work was performed as part of the Technology Transfer work being performed under Department of Energy Contract 54-7WM-335406, between December, 1984 and February, 1985. The purpose of this task team effort was to briefly survey the current use of modularization in the nuclear and non-nuclear industries and to assess and evaluate the techniques available for potential application to nuclear power. A key conclusion of the evaluation was that there was a need for a study to establish guidelines for the future development of Light Water Reactor, High Temperature Gas Reactor and Liquid Metal Reactor plants. The guidelines should identify how modularization can improve construction, maintenance, life extension and decommissioning

  2. 10 CFR 770.8 - May DOE transfer real property at defense nuclear facilities for economic development at less...

    Science.gov (United States)

    2010-01-01

    ... facilities for economic development at less than fair market value? 770.8 Section 770.8 Energy DEPARTMENT OF ENERGY TRANSFER OF REAL PROPERTY AT DEFENSE NUCLEAR FACILITIES FOR ECONOMIC DEVELOPMENT § 770.8 May DOE transfer real property at defense nuclear facilities for economic development at less than fair...

  3. Transferring knowledge and know-how from the nuclear power community to the research reactor community

    International Nuclear Information System (INIS)

    Question What is the best way of transferring knowledge and know-how from the nuclear power community to the research reactor community, e.g. in the fields of quality assurance, safety culture, etc.? To answer the question on how to transfer knowledge and know-how from the nuclear power community to the research reactor community, one should first try to establish what are the differences and similarities between these types of nuclear facilities. Despite the big difference between the primary objectives of these two kinds of facilities, i.e. electricity production versus providing irradiation services, the underlying safety culture should be comparable. For historical reasons, nuclear power plant management took the lead in establishing fully accepted safety standards. However, research reactors can avail themselves of the wide body of nuclear safety experience accumulated at nuclear power plants. This should be applicable to all nuclear facilities. Nonetheless, in transferring their know-how, safety specialists should take into account the huge differences between critical assemblies, university reactors, small research reactors and multi-purpose high power research reactors. The goal to which a specific facility is dedicated bears heavily upon the outlook of its management Question: How can well run research reactors help problem research reactors? To answer this, a basic question should in turn be posed: Should one help a research reactor with operational difficulties? And, if so, to what extent? Who will benefit? Within the framework of this meeting, one should concentrate on nuclear safety, which is determined by: Safety culture (including quality assurance); The level of training of all staff; Ageing (installation, staff and documentation); The front/back end of the fuel cycle; A strong programme versus extended shutdown; Regulatory (nuclear regulatory) inspectorates; National (international) co-operation; The financial situation prevailing at the

  4. Perspectives of heat transfer enhancement in nuclear reactors toward nanofluids applications

    Energy Technology Data Exchange (ETDEWEB)

    Rocha, Marcelo S.; Cabral, Eduardo L.L.; Sabundjian, Gaiane, E-mail: msrocha@ipen.br, E-mail: elcabral@ipen.br, E-mail: gdjian@ipen.br [Instituto de Pesquisas Energeticas e Nucleares (IPEN/CNEN-SP), Sao Paulo, SP (Brazil); and others

    2013-07-01

    Nanofluids are colloidal suspensions of nanoparticles in a base fluid with interesting physical properties and large potential for heat transfer enhancement in thermal systems among other applications. There are an increasing number of nanofluids investigations concerning many aspects of synthesis and fabrication technologies, physical properties, and special applications. Results demonstrate that physical properties like high thermal conductivities and high critical heat flux (CHF) of some nanofluids classifies them as potential working fluids for high heat flux transportation in special systems, including thermal management of microelectronic devices (MEMS) and nuclear reactors. Understanding the importance of such investigations for the knowledge development of nuclear engineering a new research is being conducted at the Nuclear Engineering Center (CEN) of the Nuclear and Energy Research Institute (IPEN/CNEN-SP) to analyze the application potentiality of some nanofluids in nuclear systems for heat transfer enhancement under ionizing radiation influence. In this work a revision of theoretical and experimental studies of nanofluids is performed and its potentiality for using in future generations of nuclear reactors is highlighted showing the status of the research at present. (author)

  5. Nuclear and microtubule remodeling and in vitro development of nuclear transferred cat oocytes with skin fibroblasts of the domestic cat (Felis silvestris catus) and leopard cat (Prionailurus bengalensis).

    Science.gov (United States)

    Yin, X J; Lee, Y H; Jin, J Y; Kim, N H; Kong, I K

    2006-10-01

    The leopard cat (Prionailurus bengalensis), a member of the felidae family, is a threatened animal in South Korea. In terms of protecting endangered felids, nuclear transfer (NT) is a potentially valuable technique for assuring the continuation of species with dwindling numbers. In the present experiment, nuclear and microtubule remodeling and the in vitro developmental potential of enucleated domestic cat oocytes reconstructed with nuclei of somatic cells from either domestic cat fibroblast (DCF) or leopard cat fibroblast (LCF) were evaluated. Microtubule aster is allocated to de-condensed chromatin following nuclear transfer (3h after activation) of fibroblast cells from both domestic and leopard cats, suggesting the introduction of a somatic cell centrosome. The transferred fibroblast nuclei formed a large, swollen, pronuclear-like structure in most reconstructed oocytes, in the cat or leopard cat. At 18h following nuclear transfer, mitosis occurred, and according to the photo (F) it appears that spindle microtubules and two asters were observed. The percentages of blastocyst formation from nuclear transfer embryos derived from domestic cat fibroblasts (4/46, 8.6%) were not significantly different than those for nuclear transfer embryos constructed with leopard cat fibroblasts (4/52, 7.6%). These results indicate that nuclear and microtubule remodeling processes and in vitro developmental ability are similar in reconstructed cat oocytes following transfer of nuclei from either domestic or leopard cats. PMID:16310987

  6. Analysis of cat oocyte activation methods for the generation of feline disease models by nuclear transfer

    Directory of Open Access Journals (Sweden)

    Herrick Jason R

    2009-12-01

    Full Text Available Abstract Background Somatic cell nuclear transfer in cats offers a useful tool for the generation of valuable research models. However, low birth rates after nuclear transfer hamper exploitation of the full potential of the technology. Poor embryo development after activation of the reconstructed oocytes seems to be responsible, at least in part, for the low efficiency. The objective of this study was to characterize the response of cat oocytes to various stimuli in order to fine-tune existing and possibly develop new activation methods for the generation of cat disease models by somatic cell nuclear transfer. Methods First, changes in the intracellular free calcium concentration [Ca2+]i in the oocytes induced by a number of artificial stimuli were characterized. The stimuli included electroporation, ethanol, ionomycin, thimerosal, strontium-chloride and sodium (Na+-free medium. The potential of the most promising treatments (with or without subsequent incubation in the presence of cycloheximide and cytochalasin B to stimulate oocyte activation and support development of the resultant parthenogenetic embryos was then evaluated. Finally, the most effective methods were selected to activate oocytes reconstructed during nuclear transfer with fibroblasts from mucopolysaccharidosis I- and alpha-mannosidosis-affected cats. Results All treatments were able to elicit a [Ca2+]i elevation in the ooplasm with various characteristics. Pronuclear formation and development up to the blastocyst stage was most efficiently triggered by electroporation (60.5 +/- 2.9 and 11.5 +/- 1.7% and the combined thimerosal/DTT treatment (67.7 +/- 1.8 and 10.6 +/- 1.9%; incubation of the stimulated oocytes with cycloheximide and cytochalasin B had a positive effect on embryo development. When these two methods were used to activate oocytes reconstructed during nuclear transfer, up to 84.9% of the reconstructed oocytes cleaved. When the 2 to 4-cell embryos (a total of 220 were

  7. Embryonic stem cells generated by nuclear transfer of human somatic nuclei into rabbit oocytes

    Institute of Scientific and Technical Information of China (English)

    YING CHEN; QING ZHANG YANG; DA YUAN CHEN; MIN KANG WANG; JIN SONG LI; SHAO LIANG HUANG; XIANG YIN KONG; YAO ZHOU SHI; ZHI QIANG WANG; JIA HUI XIA; ZHI GAO LONG; ZHI XU HE; ZHI GANG XUE; WEN XIANG DING; HUI ZHEN SHENG; AILIAN LIU; KAI WANG; WEN WEI MAO; JIAN XIN CHU; YONG LU; ZHENG FU FANG; YING TANG SHI

    2003-01-01

    To solve the problem of immune incompatibility, nuclear transplantation has been envisaged as a means to produce cells or tissues for human autologous transplantation. Here we have derived embryonic stem cells by the transfer of human somatic nuclei into rabbit oocytes. The number of blastocysts that developed from the fused nuclear transfer was comparable among nuclear donors at ages of 5, 42, 52 and 60 years, and nuclear transfer (NT) embryonic stem cells (ntES cells) were subsequently derived from each of the four age groups. These results suggest that human somatic nuclei can form ntES cells independent of the age of the donor. The derived ntES cells are human based on karyotype, isogenicity, in situ hybridization, PGR and immunocytochemistry with probes that distinguish between the various species. The ntES cells maintain the capability of sustained growth in an undifferentiated state, and form embryoid bodies, which, on further induction, give rise to cell types such as neuron and muscle, as well as mixed cell populations that express markers representative of all three germ layers. Thus, ntES cells derived from human somatic cells by NT to rabbit eggs retain phenotypes similar to those of conventional human ES cells, including the ability to undergo multilineage cellular differentiation.

  8. Nuclear Transfer of Embryonic Cell Nuclei to Non-enucleated Eggs in Zebrafish, Danio rerio

    Directory of Open Access Journals (Sweden)

    Manabu Hattori, Hisashi Hashimoto, Ekaterina Bubenshchikova, Yuko Wakamatsu

    2011-01-01

    Full Text Available We previously established a novel method for nuclear transfer in medaka (Oryzias latipes using non-enucleated, diploidized eggs as recipients for adult somatic cell nuclei. Here we report the first attempt to apply this method to another fish species. To examine suitability of using non-enucleated eggs as recipients for nuclear transfer in the zebrafish (Danio rerio, we transferred blastula cell nuclei from a wild-type donor strain to non-enucleated, unfertilized eggs from a golden recipient strain. As a result, 31 of 184 (16.8% operated eggs developed normally and reached the adult stage. Twenty-eight (15.2% of these transplants showed wild-type phenotype and the remaining three (1.6% were golden. Except for one individual that exhibited diploid/tetraploid mosaicism, all of the wild-type nuclear transplants were either triploid or diploid. While all of 19 triploid transplants were infertile, a total of six transplants (21.4% were fertile (five of the eight diploid transplants and one transplant exhibiting ploidy mosaicism. Except for one diploid individual, all of the fertile transplants transferred both the wild-type golden gene allele (slc24a5 as well as the phenotype, the wild-type body color, to their F1 and F2 progeny in a typical Mendelian fashion. PCR analysis of slc24a5 suggested that triploidy originated from a fused nucleus in the diploid donor and haploid recipient nuclei, and that the sole origin of diploidy was the diploid donor nucleus. The results of the present study demonstrated the suitability of using non-enucleated eggs as recipients for nuclear transfer experiments in zebrafish.

  9. PENICILLIN-STREPTOMYCIN IN THE CULTURE MEDIUM DURING IN VITRO MATURATION (IVM OF BOVINE OOCYTES AFFECTS NUCLEAR MATURATION AND SUBSEQUENT EMBRYO DEVELOPMENT

    Directory of Open Access Journals (Sweden)

    SHIRAZI A

    2001-01-01

    Full Text Available Introduction: Standard concentrations of antibiotics in culture media are thought to have no detectable toxic effects on the cultured cells. However, since antibiotics are biologically active substances, the possibility that they interfere to some extent with cellular processes occurring in the cultured cells can not always be totally excluded. This study, therefore, was conducted to assess whether the presence of penicllin-streptomycin (pen-strep during in vitro maturation (IVM of bovine cumulus oocyte complexes (COCs affect nuclear and cytoplasmic maturation and subsequent embryo development. Materials and Methods: Bovine COCs were matured at 39oC in a humidified atmosphere with 5 % CO2 in air for 24 h in: 1- culture medium M 199 supplemented with 10 % FCS (Fetal calf serum, 0.05 IU/ml rhFSH (recombinant human FSH and 100 units penicillin and 100 ?g streptomycin/ ml. 2- culture medium M 199 without FCS and rhFSH in the presence of pen-strep. Cultures without antibiotics served as control. Six series of experiments, each consisted of at least 3 replicates, were performed. Results: In vitro maturation in the presence of pen-strep in culture medium supplemented with FCS and rhFSH significantly (P<0.05 increased the percentage of MII oocytes, however, when the COCs were divided, on the basis of appearance of the cumulus investment, into bright and dark groups, this effect was less obvious in both types of COCs, 76% vs 72% in bright COCs (P= 0.149 or 83% vs 80% in dark COCs (P=0.296 in treated and control groups respectively. The percentage of oocytes with type III of cortical granules (CGs distribution was not affected in the presence of pen-strep. The COCs expansion after IVM was not affected by the presence of antibiotics in culture medium. The subsequent embryo development of IVM/IVF produced ova, which were exposed to pen-strep during IVM, was significantly (P<0.05 decreased with respect to blastocyst formation by day 9. In vitro maturation in

  10. Communication received from certain Member States regarding guidelines for the export of nuclear material, equipment and technology. Nuclear transfers

    International Nuclear Information System (INIS)

    The Director General has received notes verbales dated 30 June 1995 from the Resident Representatives to the Agency of Argentina, Australia, Austria, Belgium, Bulgaria, Canada, the Czech Republic, Denmark, Finland, France, Germany, Greece, Hungary, Ireland, Italy, Japan, Luxembourg, the Netherlands, New Zealand, Norway, Poland, Portugal, Romania, the Slovak Republic, South Africa, Spain, Sweden, Switzerland, the United Kingdom of Great Britain and Northern Ireland, and the United States of America relating to the export of nuclear material, equipment and technology. The purpose of the notes verbales is to provide further information on those Governments' Guidelines for Nuclear Transfers. In the light of the wish expressed at the end of each note verbale, the text of the notes verbales is annexed hereto. The enclosure to these notes verbales is also reproduced in full in the Annex

  11. [Bovine progressive degenerative myeloencephalopathy ("Weaver syndrome") in brown Swiss x Braunvieh cattle: reproductive occurrences, results of embryo transfer].

    Science.gov (United States)

    Tenhumberg, H; Trela, T; Matzke, P; Averdunk, G; Dirksen, G

    1994-12-01

    In the time between 1989 and 1991 seven Brown Swiss heifers, which had clinical signs of the Weaver syndrome were kept at the Bavarian Institute of Animal Breeding in Grub. This was in order to investigate this hereditary trait further. The number of animals carrying this genetic defect was increased by means of embryo transfer. Both cycle observations and ovary controls by means of rectal palpation resulted largely in physiological data and findings. All seven animals responded to superovulation treatment which was induced by sequential doses of p-FSH (32 mg) or of a single dose of 2,000 IU PMSG. The donors were flushed a total of 32 times without problem. On average 5.3 ova were recovered, 2.8 of which were viable and suitable for transfer. These are statistically only 50% of the normal value in a routine ET programme. Following the transfer of fresh and frozen embryos the pregnancy rate was 53%. There was only one abortion observed from 48 pregnancies.

  12. Cumulus-specific genes are transcriptionally silent following somatic cell nuclear transfer in a mouse model*

    OpenAIRE

    Tong, Guo-qing; Heng, Boon-chin; Ng, Soon-chye

    2007-01-01

    This study investigated whether four cumulus-specific genes: follicular stimulating hormone receptor (FSHr), hyaluronan synthase 2 (Has2), prostaglandin synthase 2 (Ptgs2) and steroidogenic acute regulator protein (Star), were correctly reprogrammed to be transcriptionally silent following somatic cell nuclear transfer (SCNT) in a murine model. Cumulus cells of C57×CBA F1 female mouse were injected into enucleated oocytes, followed by activation in 10 µmol/L strontium chloride for 5 h and sub...

  13. Bioactive conformation of stromelysin inhibitors determined by transferred nuclear Overhauser effects.

    OpenAIRE

    Gonnella, N. C.; Bohacek, R; Zhang, X.; Kolossváry, I; Paris, C G; Melton, R; Winter, C; Hu, S I; Ganu, V

    1995-01-01

    The transferred nuclear Overhauser effect has been used to determine the biologically active conformations of two stromelysin inhibitors. Both inhibitors used in this study were hydroxamic acids generated via chemical synthesis. These structures, representing the conformation of each inhibitor bound to stromelysin, superimposed with excellent agreement. The study also provided information on the shape and orientation of the S2' and S1' pockets of the enzyme relative to thermolysin. Comparison...

  14. Legal aspects of nuclear technology transfer in connection with Latin America

    International Nuclear Information System (INIS)

    This paper concerns technology and technology transfers which are becoming increasingly important for developing countries, especially those in South America. The author also points out that developed countries have not implemented the United Nations resolutions concerning dissemination of knowledge on advanced technologies. He stresses that if South American States wish to obtain assistance with nuclear technology from developed countries they should sign and ratify the Non-Proliferation Treaty and the Tlatelolco Treaty. (NEA)

  15. Neutron Transfer Reactions: Surrogates for Neutron Capture for Basic and Applied Nuclear Science

    Science.gov (United States)

    Cizewski, J. A.; Jones, K. L.; Kozub, R. L.; Pain, S. D.; Peters, W. A.; Adekola, A.; Allen, J.; Bardayan, D. W.; Becker, J. A.; Blackmon, J. C.; Chae, K. Y.; Chipps, K. A.; Erikson, L.; Gaddis, A.; Harlin, C.; Hatarik, R.; Howard, J.; Jandel, M.; Johnson, M. S.; Kapler, R.; Krolas, W.; Liang, F.; Livesay, R. J.; Ma, Z.; Matei, C.; Matthews, C.; Moazen, B.; Nesaraja, C. D.; O'Malley, P.; Patterson, N.; Paulauskas, S. V.; Pelham, T.; Pittman, S. T.; Radford, D.; Rogers, J.; Schmitt, K.; Shapira, D.; Shriner, J. F.; Sissom, D. J.; Smith, M. S.; Swan, T.; Thomas, J. S.; Vieira, D. J.; Wilhelmy, J. B.; Wilson, G. L.

    2009-03-01

    Neutron capture reactions on unstable nuclei are important for both basic and applied nuclear science. A program has been developed at the Holifield Radioactive Ion Beam Facility at Oak Ridge National Laboratory to study single-neutron transfer (d,p) reactions with rare isotope beams to provide information on neutron-induced reactions on unstable nuclei. Results from (d,p) studies on 130,132Sn, 134Te and 75As are discussed.

  16. Neutron transfer reactions: Surrogates for neutron capture for basic and applied nuclear science

    Energy Technology Data Exchange (ETDEWEB)

    Cizewski, J. A. [Rutgers University; Jones, K. L. [University of Tennessee; Kozub, R. L. [Tennessee Technological University; Pain, Steven D [ORNL; Peters, W. A. [Rutgers University; Adekola, Aderemi S [ORNL; Allen, J. [Rutgers University; Bardayan, Daniel W [ORNL; Becker, J. [Lawrence Livermore National Laboratory (LLNL); Blackmon, Jeff C [ORNL; Chae, K. Y. [University of Tennessee; Chipps, K. [Colorado School of Mines, Golden; Erikson, Luke [Colorado School of Mines, Golden; Gaddis, A. L. [Furman University; Harlin, Christopher W [ORNL; Hatarik, Robert [Rutgers University; Howard, Joshua A [ORNL; Jandel, M. [Los Alamos National Laboratory (LANL); Johnson, Micah [ORNL; Kapler, R. [University of Tennessee; Krolas, W. [University of Warsaw; Liang, J Felix [ORNL; Livesay, Jake [ORNL; Ma, Zhanwen [ORNL; Matei, Catalin [Oak Ridge Associated Universities (ORAU); Matthews, C. [Rutgers University; Moazen, Brian [University of Tennessee; Nesaraja, Caroline D [ORNL; O' Malley, Patrick [Rutgers University; Patterson, N. P. [University of Surrey, UK; Paulauskas, Stanley [University of Tennessee; Pelham, T. [University of Surrey, UK; Pittman, S. T. [University of Tennessee, Knoxville (UTK); Radford, David C [ORNL; Rogers, J. [Tennessee Technological University; Schmitt, Kyle [University of Tennessee; Shapira, Dan [ORNL; ShrinerJr., J. F. [Tennessee Technological University; Sissom, D. J. [Tennessee Technological University; Smith, Michael Scott [ORNL; Swan, T. P. [University of Surrey, UK; Thomas, J. S. [Rutgers University; Vieira, D. J. [Los Alamos National Laboratory (LANL); Wilhelmy, J. B. [Los Alamos National Laboratory (LANL); Wilson, Gemma L [ORNL

    2009-04-01

    Neutron capture reactions on unstable nuclei are important for both basic and applied nuclear science. A program has been developed at the Holifield Radioactive Ion Beam Facility at Oak Ridge National Laboratory to study single-neutron transfer (d,p) reactions with rare isotope beams to provide information on neutron-induced reactions on unstable nuclei. Results from (d,p) studies on {sup 130,132}Sn, {sup 134}Te and {sup 75}As are discussed.

  17. Embryonic Development following Somatic Cell Nuclear Transfer Impeded by Persisting Histone Methylation

    OpenAIRE

    Matoba, Shogo; Liu, Yuting; Lu, Falong; Iwabuchi, Kumiko A.; Inoue, Azusa; Zhang, Yi

    2014-01-01

    Mammalian oocytes can reprogram somatic cells into a totipotent state enabling animal cloning through somatic cell nuclear transfer (SCNT). However, the majority of SCNT embryos fail to develop to term due to undefined reprogramming defects. Here we identify histone H3 lysine 9 trimethylation (H3K9me3) of donor cell genome as a major epigenetic barrier for efficient reprogramming by SCNT. Comparative transcriptome analysis identified reprogramming resistant regions (RRRs) that are expressed n...

  18. Privileged Communication Embryonic Development Following Somatic Cell Nuclear Transfer Impeded by Persisting Histone Methylation

    OpenAIRE

    Matoba, Shogo; Liu, Yuting; Lu, Falong; Iwabuchi, Kumiko A.; Shen, Li; Inoue, Azusa; Zhang, Yi

    2014-01-01

    Mammalian oocytes can reprogram somatic cells into a totipotent state enabling animal cloning through somatic cell nuclear transfer (SCNT). However, the majority of SCNT embryos fail to develop to term due to undefined reprogramming defects. Here we identify histone H3 lysine 9 trimethylation (H3K9me3) of donor cell genome as a major epigenetic barrier for efficient reprogramming by SCNT. Comparative transcriptome analysis identified reprogramming resistant regions (RRRs) that are expressed n...

  19. Progress toward generating a ferret model of cystic fibrosis by somatic cell nuclear transfer

    OpenAIRE

    Engelhardt John F; Li Ziyi

    2003-01-01

    Abstract Mammalian cloning by nuclear transfer from somatic cells has created new opportunities to generate animal models of genetic diseases in species other than mice. Although genetic mouse models play a critical role in basic and applied research for numerous diseases, often mouse models do not adequately reproduce the human disease phenotype. Cystic fibrosis (CF) is one such disease. Targeted ablation of the cystic fibrosis transmembrane conductance regulator (CFTR) gene in mice does not...

  20. Production of Cloned Korean Native Pig by Somatic Cell Nuclear Transfer

    OpenAIRE

    Hwang, In-Sul; Kwon, Dae-Jin; Oh, Keun Bong; Ock, Sun-A; Chung, Hak-Jae; Cho, In-Cheol; Lee, Jeong-Woong; Im, Gi-Sun; Hwang, Seongsoo

    2015-01-01

    The Korean native pig (KNP) have been considered as animal models for animal biotechnology research because of their relatively small body size and their presumably highly inbred status due to the closed breeding program. However, little is reported about the use of KNP for animal biotechnology researches. This study was performed to establish the somatic cell nuclear transfer (SCNT) protocol for the production of swine leukocyte antigens (SLA) homotype-defined SCNT KNP. The ear fibroblast ce...

  1. Ion engine propelled Earth-Mars cycler with nuclear thermal propelled transfer vehicle, volume 2

    Science.gov (United States)

    Meyer, Rudolf X.; Baker, Myles; Melko, Joseph

    1994-01-01

    The goal of this project was to perform a preliminary design of a long term, reusable transportation system between earth and Mars which would be capable of providing both artificial gravity and shelter from solar flare radiation. The heart of this system was assumed to be a Cycler spacecraft propelled by an ion propulsion system. The crew transfer vehicle was designed to be propelled by a nuclear-thermal propulsion system. Several Mars transportation system architectures and their associated space vehicles were designed.

  2. A protocol for embryonic stem cell derivation by somatic cell nuclear transfer into human oocytes

    OpenAIRE

    sprotocols

    2014-01-01

    Authors: Dieter Egli & Gloryn Chia ### Abstract Here we describe detailed methods that allowed us to derive embryonic stem cell lines by nuclear transfer of fibroblasts from a newborn and from a type 1 diabetic adult. The protocol is based on the insight that 1) agents for cell fusion can act as potent mediators of oocyte activation by compromising maintaining plasma membrane integrity; minimizing the concentration at which they are used, and at least transiently remove calcium f...

  3. Production of human CD59-transgenic pigs by embryonic germ cell nuclear transfer

    International Nuclear Information System (INIS)

    Research highlights: → Human CD59 (hCD59) gene was introduced into porcine embryonic germ (EG) cells. → hCD59-transgenic EG cells were resistant to hyperacute rejection in cytolytic assay. → hCD59-transgenic pigs were produced by EG cell nuclear transfer. -- Abstract: This study was performed to produce transgenic pigs expressing the human complement regulatory protein CD59 (hCD59) using the nuclear transfer (NT) of embryonic germ (EG) cells, which are undifferentiated stem cells derived from primordial germ cells. Because EG cells can be cultured indefinitely in an undifferentiated state, they may provide an inexhaustible source of nuclear donor cells for NT to produce transgenic pigs. A total of 1980 NT embryos derived from hCD59-transgenic EG cells were transferred to ten recipients, resulting in the birth of fifteen piglets from three pregnancies. Among these offspring, ten were alive without overt health problems. Based on PCR analysis, all fifteen piglets were confirmed as hCD59 transgenic. The expression of the hCD59 transgene in the ten living piglets was verified by RT-PCR. Western analysis showed the expression of the hCD59 protein in four of the ten RT-PCR-positive piglets. These results demonstrate that hCD59-transgenic pigs could effectively be produced by EG cell NT and that such transgenic pigs may be used as organ donors in pig-to-human xenotransplantation.

  4. Production of human CD59-transgenic pigs by embryonic germ cell nuclear transfer

    Energy Technology Data Exchange (ETDEWEB)

    Ahn, Kwang Sung; Won, Ji Young [Department of Physiology, Dankook University School of Medicine, Cheonan (Korea, Republic of); Park, Jin-Ki [Animal Biotechnology Division, National Institute of Animal Science, Suwon (Korea, Republic of); Sorrell, Alice M. [Department of Physiology, Dankook University School of Medicine, Cheonan (Korea, Republic of); Heo, Soon Young; Kang, Jee Hyun [Department of Nanobiomedical Science, Dankook University, Cheonan (Korea, Republic of); Woo, Jae-Seok [Animal Biotechnology Division, National Institute of Animal Science, Suwon (Korea, Republic of); Choi, Bong-Hwan [Genomics and Bioinformatics Division, National Institute of Animal Science, Suwon (Korea, Republic of); Chang, Won-Kyong [Animal Biotechnology Division, National Institute of Animal Science, Suwon (Korea, Republic of); Shim, Hosup, E-mail: shim@dku.edu [Department of Nanobiomedical Science, Dankook University, Cheonan (Korea, Republic of); Institute of Tissue Regeneration Engineering, Dankook University, Cheonan (Korea, Republic of)

    2010-10-01

    Research highlights: {yields} Human CD59 (hCD59) gene was introduced into porcine embryonic germ (EG) cells. {yields} hCD59-transgenic EG cells were resistant to hyperacute rejection in cytolytic assay. {yields} hCD59-transgenic pigs were produced by EG cell nuclear transfer. -- Abstract: This study was performed to produce transgenic pigs expressing the human complement regulatory protein CD59 (hCD59) using the nuclear transfer (NT) of embryonic germ (EG) cells, which are undifferentiated stem cells derived from primordial germ cells. Because EG cells can be cultured indefinitely in an undifferentiated state, they may provide an inexhaustible source of nuclear donor cells for NT to produce transgenic pigs. A total of 1980 NT embryos derived from hCD59-transgenic EG cells were transferred to ten recipients, resulting in the birth of fifteen piglets from three pregnancies. Among these offspring, ten were alive without overt health problems. Based on PCR analysis, all fifteen piglets were confirmed as hCD59 transgenic. The expression of the hCD59 transgene in the ten living piglets was verified by RT-PCR. Western analysis showed the expression of the hCD59 protein in four of the ten RT-PCR-positive piglets. These results demonstrate that hCD59-transgenic pigs could effectively be produced by EG cell NT and that such transgenic pigs may be used as organ donors in pig-to-human xenotransplantation.

  5. Modeling of the heat transfer performance of plate-type dispersion nuclear fuel elements

    Science.gov (United States)

    Ding, Shurong; Huo, Yongzhong; Yan, XiaoQing

    2009-08-01

    Considering the mutual actions between fuel particles and the metal matrix, the three-dimensional finite element models are developed to simulate the heat transfer behaviors of dispersion nuclear fuel plates. The research results indicate that the temperatures of the fuel plate might rise more distinctly with considering the particle swelling and the degraded surface heat transfer coefficients with increasing burnup; the local heating phenomenon within the particles appears when their thermal conductivities are too low. With rise of the surface heat transfer coefficients, the temperatures within the fuel plate decrease; the temperatures of the fuel plate are sensitive to the variations of the heat transfer coefficients whose values are lower, but their effects are weakened and slight when the heat transfer coefficients increase and reach a certain extent. Increasing the heat generation rate leads to elevating the internal temperatures. The temperatures and the maximum temperature differences within the plate increase along with the particle volume fractions. The surface thermal flux goes up along with particle volume fractions and heat generation rates, but the effects of surface heat transfer coefficients are not evident.

  6. Artificial intelligence and nuclear power. Report by the Technology Transfer Artificial Intelligence Task Team

    International Nuclear Information System (INIS)

    The Artificial Intelligence Task Team was organized to review the status of Artificial Intelligence (AI) technology, identify guidelines for AI work, and to identify work required to allow the nuclear industry to realize maximum benefit from this technology. The state of the nuclear industry was analyzed to determine where the application of AI technology could be of greatest benefit. Guidelines and criteria were established to focus on those particular problem areas where AI could provide the highest possible payoff to the industry. Information was collected from government, academic, and private organizations. Very little AI work is now being done to specifically support the nuclear industry. The AI Task Team determined that the establishment of a Strategic Automation Initiative (SAI) and the expansion of the DOE Technology Transfer program would ensure that AI technology could be used to develop software for the nuclear industry that would have substantial financial payoff to the industry. The SAI includes both long and short term phases. The short-term phase includes projects which would demonstrate that AI can be applied to the nuclear industry safely, and with substantial financial benefit. The long term phase includes projects which would develop AI technologies with specific applicability to the nuclear industry that would not be developed by people working in any other industry

  7. Nonideal rotations in nuclear magnetic resonance: Estimation of coherence transfer leakage

    Energy Technology Data Exchange (ETDEWEB)

    Jerschow, Alexej [Materials Sciences Division, 11-D62, Lawrence Berkeley Laboratory, Berkeley, California 94720 (United States); Chemistry Department, D62 Hildebrand, University of California at Berkeley, Berkeley, California 94720 (United States)

    2000-07-15

    When spherical tensors are rotated by certain angles, coherence transfer selection rules may apply. For example, a {pi} rotation cleanly inverts the coherence order. A {pi}/2 rotation of a T{sub 0}{sup 1} tensor creates only T{sub {+-}}{sub 1}{sup 1} tensors. In this work estimations are given for the coherence transfer leakage under the action of rotations with small errors in the rotation angle or axis. Although the theory is stated with particular applications to NMR (nuclear magnetic resonance) in mind it is equally applicable wherever nonideal rotations of spherical tensors are considered (e.g., quantum computing and relaxation theory). In NMR it is useful for the estimation of coherence transfer leakage, especially in pulse sequences with many n{pi} pulses. The results are also applicable to spinors and half-integer representations of the rotation group. (c) 2000 American Institute of Physics.

  8. Reprogramming within hours following nuclear transfer into mouse but not human zygotes.

    Science.gov (United States)

    Egli, Dieter; Chen, Alice E; Saphier, Genevieve; Ichida, Justin; Fitzgerald, Claire; Go, Kathryn J; Acevedo, Nicole; Patel, Jay; Baetscher, Manfred; Kearns, William G; Goland, Robin; Leibel, Rudolph L; Melton, Douglas A; Eggan, Kevin

    2011-01-01

    Fertilized mouse zygotes can reprogram somatic cells to a pluripotent state. Human zygotes might therefore be useful for producing patient-derived pluripotent stem cells. However, logistical, legal and social considerations have limited the availability of human eggs for research. Here we show that a significant number of normal fertilized eggs (zygotes) can be obtained for reprogramming studies. Using these zygotes, we found that when the zygotic genome was replaced with that of a somatic cell, development progressed normally throughout the cleavage stages, but then arrested before the morula stage. This arrest was associated with a failure to activate transcription in the transferred somatic genome. In contrast to human zygotes, mouse zygotes reprogrammed the somatic cell genome to a pluripotent state within hours after transfer. Our results suggest that there may be a previously unappreciated barrier to successful human nuclear transfer, and that future studies could focus on the requirements for genome activation. PMID:21971503

  9. Heat transfer in a non adiabatic calorimeter: numerical parametric study without and with nuclear heating deposit

    International Nuclear Information System (INIS)

    The numerical works presented in this paper belong to the IN-CORE (Instrumentation for Nuclear radiations and Calorimetry Online in REactor) research program. Its scientific aim is to create a new device dedicated to the online simultaneously measurements of nuclear conditions inside experimental channels of the Jules Horowitz Reactor (JHR) by coupling different sensors. This paper studies a specific one: a radiometric calorimeter used to in pile nuclear heating measurements. Numerical simulations on heat transfers taking place into this sensor under radioactive and nonradioactive conditions are carried out. The influence of the geometrical dimensions and of the energy deposit on the heat flux density, on the sensor sensitivity and on the maximum temperature is discussed. (author)

  10. Heat transfer analysis of fuel assemblies in a heterogeneous gas core nuclear rocket

    Science.gov (United States)

    Watanabe, Yoichi; Appelbaum, Jacob; Diaz, Nils; Maya, Isaac

    1991-01-01

    Heat transfer problems of a heterogeneous gaseous core nuclear rocket were studied. The reactor core consists of 1.5-m long hexagonal fuel assemblies filled with pressurized uranium tetrafluoride (UF4) gas. The fuel gas temperature ranges from 3500 to 7000 K at a nominal operating condition of 40 atm. Each fuel assembly has seven coolant tubes, through which hydrogen propellant flows. The propellant temperature is not constrained by the fuel temperature but by the maximum temperature of the graphite coolant tube. For a core achieving a fission power density of 1000 MW/cu m, the propellant core exit temperature can be as high as 3200 K. The physical size of a 1250 MW gaseous core nuclear rocket is comparable with that of a NERVA-type solid core nuclear rocket. The engine can deliver a specific impulse of 1020 seconds and a thrust of 330 kN.

  11. Technology transfer of nuclear techniques and nucleonic control systems in the mineral industry

    International Nuclear Information System (INIS)

    Among the many beneficial applications of radiation and radioisotopes in industry which are now well established in advanced countries, the applications of nuclear techniques and nucleonic control systems in the mineral industry have great potential for developing Member States. The use of nucleonic on-stream analyzers in the coal industry has resulted in enormous technical and economic benefits in addition to minimization of environmental pollution. Large savings have also resulted from the use of such analyzers in the processing of other minerals. Nuclear borehole logging techniques have demonstrated great potential in oil and gas evaluation. Radiotracer investigations have led to process optimisation and trouble shooting in various stages in ore processing and metallurgy. Though the technical and economic benefits of applications of nuclear techniques in the mineral industry are well recognised, technology transfer in these areas has been hampered by a variety of factors. In order to review the status and trends in nuclear techniques and nucleonic control systems in the mineral industry and the problems and considerations in their technology transfer to developing Member States, the IAEA convened an Advisory Group Meeting in Bombay, India, 15-19 January 1990. The present publication is based on the 7 contributions presented at this meeting. A separate abstract was prepared for each of these papers. Refs, figs and tabs

  12. Coherent transfer of nuclear spin polarization in field-cycling NMR experiments.

    Science.gov (United States)

    Pravdivtsev, Andrey N; Yurkovskaya, Alexandra V; Vieth, Hans-Martin; Ivanov, Konstantin L

    2013-12-28

    Coherent polarization transfer effects in a coupled spin network have been studied over a wide field range. The transfer mechanism is based on exciting zero-quantum coherences between the nuclear spin states by means of non-adiabatic field jump from high to low magnetic field. Subsequent evolution of these coherences enables conversion of spin order in the system, which is monitored after field jump back to high field. Such processes are most efficient when the spin system passes through an avoided level crossing during the field variation. The polarization transfer effects have been demonstrated for N-acetyl histidine, which has five scalar coupled protons; the initial spin order has been prepared by applying RF-pulses at high magnetic field. The observed oscillatory transfer kinetics is taken as a clear indication of a coherent mechanism; level crossing effects have also been demonstrated. The experimental data are in very good agreement with the theoretical model of coherent polarization transfer. The method suggested is also valid for other types of initial polarization in the spin system, most notably, for spin hyperpolarization. PMID:24387362

  13. Coherent transfer of nuclear spin polarization in field-cycling NMR experiments

    Energy Technology Data Exchange (ETDEWEB)

    Pravdivtsev, Andrey N.; Yurkovskaya, Alexandra V.; Ivanov, Konstantin L., E-mail: ivanov@tomo.nsc.ru [International Tomography Center, Siberian Branch of the Russian Academy of Science, Institutskaya 3a, Novosibirsk 630090 (Russian Federation); Novosibirsk State University, Pirogova 2, Novosibirsk 630090 (Russian Federation); Vieth, Hans-Martin [Institut für Experimental Physik, Freie Universität Berlin, Arnimallee 14, Berlin 14195 (Germany)

    2013-12-28

    Coherent polarization transfer effects in a coupled spin network have been studied over a wide field range. The transfer mechanism is based on exciting zero-quantum coherences between the nuclear spin states by means of non-adiabatic field jump from high to low magnetic field. Subsequent evolution of these coherences enables conversion of spin order in the system, which is monitored after field jump back to high field. Such processes are most efficient when the spin system passes through an avoided level crossing during the field variation. The polarization transfer effects have been demonstrated for N-acetyl histidine, which has five scalar coupled protons; the initial spin order has been prepared by applying RF-pulses at high magnetic field. The observed oscillatory transfer kinetics is taken as a clear indication of a coherent mechanism; level crossing effects have also been demonstrated. The experimental data are in very good agreement with the theoretical model of coherent polarization transfer. The method suggested is also valid for other types of initial polarization in the spin system, most notably, for spin hyperpolarization.

  14. Technological requirements of nuclear electric propulsion systems for fast Earth-Mars transfers

    Science.gov (United States)

    Bérend, N.; Epenoy, R.; Cliquet, E.; Laurent-Varin, J.; Avril, S.

    2013-03-01

    Recent advances in electric propulsion technologies such as magnetoplasma rockets gave a new momentum to the study of nuclear electric propulsion concepts for Mars missions. Some recent works have been focused on very short Earth-to-Mars transfers of about 40 days with high-power, variable specific impulse propulsion systems [1]. While the interest of nuclear electric propulsion appears clearly with regard to the payload mass ratio (due to a high level of specific impulse), its interest with regard to the transfer time is more complex to define, as it depends on many design parameters. In this paper, a general analysis of the capability of nuclear electric propulsion systems considering both criteria (the payload mass ratio and the transfer time) is performed, and the technological requirements for fast Earth-Mars transfers are studied. This analysis has been performed in two steps. First, complete trajectory optimizations have been performed by CNES-DCT in order to obtain the propulsion requirements of the mission for different technological hypotheses regarding the engine technology (specific impulse levels and the throttling capability) and different mission requirements. The methodology used for designing fuel-optimal heliocentric trajectories, based on the Pontryagin's Maximum Principle will be presented. Trajectories have been computed for various power levels combined with either variable or fixed Isp. The second step consisted in evaluating a simpler method that could easily link the main mission requirements (the transfer time and the payload fraction) to the main technological requirements (the specific mass of the power generation system and the structure mass ratio of the whole vehicle, excluding the power generation system). Indeed, for power-limited systems, propulsion requirements can be characterized through the "trajectory characteristic" parameter, defined as the integral over time of the squared thrust acceleration. Technological requirements for

  15. Green fluorescent protein (GFP) transgenic pig produced by somatic cell nuclear transfer

    Institute of Scientific and Technical Information of China (English)

    LIU ZhongHua; SUN Shuang; LI YuTian; WANG HongBin; R S PRATHER; SONG Jun; WANG ZhenKun; TIAN JiangTian; KONG QingRan; ZHENG Zhong; YIN Zhi; GAO Li; MA HaiKun

    2008-01-01

    Transgenic somatic cell nuclear transfer is a very promising route for producing transgenic farm ani-mals. Research on GFP transgenic pigs can provide useful information for breeding transgenic pigs, human disease models and human organ xenotransplantation. In this study, a liposomal transfection system was screened and transgenic embryos were reconstructed by nuclear transfer of GFP positive cells into enucleated in vitro matured oocytes. The development of reconstructed embryos both in vitro and in vivo was observed, and GFP expression was determined. The results showed that porcine fe-tal-derived fibroblast cells cultured with 4.0 plJmL liposome and 1.6 pg/mL plasmid DNA for 6 h re-sulted in the highest transfection rate (3.6%). The percentage of GFP reconstructed embryos that de-veloped in vitro to the blastocyst stage was 10%. Of those the GFP positive percentage was 48%. Re-constructed transgenic embryos were transferred to 10 recipients. 5 of them were pregnant, and 3 de-livered 6 cloned piglets in which 4 piglets were transgenic for the GFP as verified by both GFP protein expression and GFP DNA sequence analysis. The percentage of reconstructed embryos that resulted in cloned piglets was 1.0%; while the percentage of piglets that were transgenic was 0.7%. This is the first group of transgenic cloned pigs born in China, marking a great progress in Chinese transgenic cloned pig research.

  16. Technology transfer on long-term radioactive waste management - a feasible option for small nuclear programmes?

    International Nuclear Information System (INIS)

    The EU project CATT - Co-operation and technology transfer on long-term radioactive waste management for Member States with small nuclear programmes investigated the feasibility of countries with small nuclear programmes implementing long-term radioactive waste management solutions within their national borders, through collaboration on technology transfer with those countries with advanced disposal concepts. The main project objective was to analyse the existing capabilities of technology owning Member States and the corresponding requirements of potential technology acquiring Member States and, based on the findings, to develop a number of possible collaboration models and scenarios that could be used in a technology transfer scheme. The project CATT was performed as a specific support action under the EU sixth framework programme and it brought together waste management organisations from six EU Member States: UK, Bulgaria, Germany, Lithuania, Slovenia and Sweden. In addition, the EC Joint Research Centre from the Netherlands also participated as a full partner. The paper summarises the analyses performed and the results obtained within the project. (author)

  17. Identification of Nuclear Effects in Neutrino-Carbon Interactions at Low Three-Momentum Transfer

    Science.gov (United States)

    Rodrigues, P. A.; Demgen, J.; Miltenberger, E.; Aliaga, L.; Altinok, O.; Bellantoni, L.; Bercellie, A.; Betancourt, M.; Bodek, A.; Bravar, A.; Budd, H.; Cai, T.; Carneiro, M. F.; Chvojka, J.; Devan, J.; Dytman, S. A.; Díaz, G. A.; Eberly, B.; Elkins, M.; Felix, J.; Fields, L.; Fine, R.; Gago, A. M.; Galindo, R.; Gallagher, H.; Ghosh, A.; Golan, T.; Gran, R.; Harris, D. A.; Higuera, A.; Hurtado, K.; Kiveni, M.; Kleykamp, J.; Kordosky, M.; Le, T.; Leistico, J. R.; Lovlein, A.; Maher, E.; Manly, S.; Mann, W. A.; Marshall, C. M.; Martinez Caicedo, D. A.; McFarland, K. S.; McGivern, C. L.; McGowan, A. M.; Messerly, B.; Miller, J.; Mislivec, A.; Morfín, J. G.; Mousseau, J.; Muhlbeier, T.; Naples, D.; Nelson, J. K.; Norrick, A.; Nuruzzaman; Osta, J.; Paolone, V.; Patrick, C. E.; Perdue, G. N.; Ramirez, M. A.; Ransome, R. D.; Ray, H.; Ren, L.; Rimal, D.; Ruterbories, D.; Schellman, H.; Schmitz, D. W.; Solano Salinas, C. J.; Tagg, N.; Tice, B. G.; Valencia, E.; Walton, T.; Wolcott, J.; Wospakrik, M.; Zavala, G.; Zhang, D.; Minerva Collaboration

    2016-02-01

    Two different nuclear-medium effects are isolated using a low three-momentum transfer subsample of neutrino-carbon scattering data from the MINERvA neutrino experiment. The observed hadronic energy in charged-current νμ interactions is combined with muon kinematics to permit separation of the quasielastic and Δ (1232 ) resonance processes. First, we observe a small cross section at very low energy transfer that matches the expected screening effect of long-range nucleon correlations. Second, additions to the event rate in the kinematic region between the quasielastic and Δ resonance processes are needed to describe the data. The data in this kinematic region also have an enhanced population of multiproton final states. Contributions predicted for scattering from a nucleon pair have both properties; the model tested in this analysis is a significant improvement but does not fully describe the data. We present the results as a double-differential cross section to enable further investigation of nuclear models. Improved description of the effects of the nuclear environment are required by current and future neutrino oscillation experiments.

  18. Coupled electron-nuclear dynamics: Charge migration and charge transfer initiated near a conical intersection

    Science.gov (United States)

    Mendive-Tapia, David; Vacher, Morgane; Bearpark, Michael J.; Robb, Michael A.

    2013-07-01

    Coupled electron-nuclear dynamics, implemented using the Ehrenfest method, has been used to study charge migration with fixed nuclei, together with charge transfer when nuclei are allowed to move. Simulations were initiated at reference geometries of neutral benzene and 2-phenylethylamine (PEA), and at geometries close to potential energy surface crossings in the cations. Cationic eigenstates, and the so-called sudden approximation, involving removal of an electron from a correlated ground-state wavefunction for the neutral species, were used as initial conditions. Charge migration without coupled nuclear motion could be observed if the Ehrenfest simulation, using the sudden approximation, was started near a conical intersection where the states were both strongly coupled and quasi-degenerate. Further, the main features associated with charge migration were still recognizable when the nuclear motion was allowed to couple. In the benzene radical cation, starting from the reference neutral geometry with the sudden approximation, one could observe sub-femtosecond charge migration with a small amplitude, which results from weak interaction with higher electronic states. However, we were able to engineer large amplitude charge migration, with a period between 10 and 100 fs, corresponding to oscillation of the electronic structure between the quinoid and anti-quinoid cationic electronic configurations, by distorting the geometry along the derivative coupling vector from the D6h Jahn-Teller crossing to lower symmetry where the states are not degenerate. When the nuclear motion becomes coupled, the period changes only slightly. In PEA, in an Ehrenfest trajectory starting from the D2 eigenstate and reference geometry, a partial charge transfer occurs after about 12 fs near the first crossing between D1, D2 (N+-Phenyl, N-Phenyl+). If the Ehrenfest propagation is started near this point, using the sudden approximation without coupled nuclear motion, one observes an

  19. The transfer of the nuclear supervision into the federal self-administration in the view of the constitutional law

    International Nuclear Information System (INIS)

    The paper is focussed on the question of a possible transfer of the nuclear supervision from the federal executive administration into a federal self-administration. The discussed topics include the characterization of the nuclear supervision tasks, the relation between administrative tasks and the type of administration, an assessment of the precondition of centrality with respect to the nuclear supervision and a possible accomplishment of the so called centrality

  20. Role of hydrogen-bonding and photoinduced electron transfer (PET) on the interaction of resorcinol based acridinedione dyes with Bovine Serum Albumin (BSA) in water

    Energy Technology Data Exchange (ETDEWEB)

    Kumaran, Rajendran, E-mail: kumaranwau@rediffmail.com [Department of Chemistry, Dwaraka Doss Goverdhan Doss, Vaishnav College (Autonomous), 833, Gokul Bagh, E.V.R. Periyar Road, Arumbakkam, Chennai 600106, Tamil Nadu (India); Vanjinathan, Mahalingam [Department of Chemistry, Dwaraka Doss Goverdhan Doss, Vaishnav College (Autonomous), 833, Gokul Bagh, E.V.R. Periyar Road, Arumbakkam, Chennai 600106, Tamil Nadu (India); Ramamurthy, Perumal [National Centre for Ultrafast Processes, University of Madras, Taramani Campus Chennai 600113, Tamil Nadu (India)

    2015-08-15

    Resorcinol based acridinedione (ADDR) dyes are a class of laser dyes and have structural similarity with purine derivatives, nicotinamide adenine dinucleotide (NADH) analogs. These dyes are classified into photoinduced electron transfer (PET) and non-photoinduced electron transfer dyes, and the photophysical properties of family of these dyes exhibiting PET behavior are entirely different from that of non-PET dyes. The PET process in ADDR dyes is governed by the solvent polarity such that an ADDR dye exhibits PET process through space in an aprotic solvent like acetonitrile and does not exhibit the same in protic solvents like water and methanol. A comparison on the fluorescence emission, lifetime and nature of interaction of various ADDR dyes with a large globular protein like Bovine Serum Albumin (BSA) was carried out in aqueous solution. The interaction of PET based ADDR dyes with BSA in water is found to be largely hydrophobic, but hydrogen-bonding interaction of BSA with dye molecule influences the fluorescence emission of the dye and shifts the emission towards red region. Fluorescence spectral studies reveal that the excited state properties of PET based ADDR dyes are largely influenced by the addition of BSA. The microenvironment around the dye results in significant change in the fluorescence lifetime and emission. Fluorescence enhancement with a red shift in the emission results after the addition of BSA to ADDR dyes containing free amino hydrogen in the 10th position of basic acridinedione dye. The amino hydrogen (N–H) in the 10th position of ADDR dye is replaced by methyl group (N–CH{sub 3}), a significant decrease in the fluorescence intensity with no apparent shift in the emission maximum was observed after the addition of BSA. The nature of interaction between ADDR dyes with BSA is hydrogen-bonding and the dye remains unbound even at the highest concentration of BSA. Circular Dichroism (CD) studies show that the addition of dye to BSA results in

  1. Conservation of the Sapsaree (Canis familiaris), a Korean Natural Monument, using somatic cell nuclear transfer.

    Science.gov (United States)

    Jang, Goo; Hong, SoGun; Kang, JungTaek; Park, JungEun; Oh, HyunJu; Park, ChanKyu; Ha, JiHong; Kim, DaeYong; Kim, MinKyu; Lee, ByeongChun

    2009-09-01

    A recent emerging technology, somatic cell nuclear transfer (SCNT), has been considered for conserving threatened or endangered species. Sapsaree is a native breed in Korea and has been designated as a Natural Monument. The aim of this study was to produce a Sapsaree by SCNT for breed conservation. Donor fibroblasts from a 9-year-old male Sapsaree were placed into the perivitelline spaces of enucleated in vivo matured oocytes and fused electrically. A total of 309 cloned embryos were transferred into the oviducts of 15 naturally synchronized recipients. Two recipients were diagnosed as pregnant, and each delivered one cloned puppy, both of which weighed 530 g. Overall, this study demonstrated that an endangered canine breed can be conserved by SCNT.

  2. Effects of stabilizers on the heat transfer characteristics of a nuclear waste canister

    International Nuclear Information System (INIS)

    This report summarizes the feasibility and the effectiveness of using stabilizers (internal metal structural components) to augment the heat transfer characteristics of a nuclear waste canister. The problem was modeled as a transient two-dimensional heat transfer in two physical domains - the stabilizer and the wedge (a 30-degree-angle canister segment), which includes the heat-producing spent-fuel rods. This problem is solved by a simultaneous and interrelated numerical investigation of the two domains in cartesian and polar coordinate systems. The numerical investigations were performed for three cases. In the first case, conduction was assumed to be the dominant mechanism for heat transfer. The second case assumed that radiation was the dominant mechanism, and in the third case both radiation and conduction were considered as mechanisms of heat transfer. The results show that for typical conditions in a waste package design, the stabilizers are quite effective in reducing the overall temperature in a waste canister. Furthermore, the results show that increasing the stabilizer thickness over the thickness specified in the present design has a negligible effect on the temperature distribution in the canister. Finally, the presence of the stabilizers was found to shift the location of the peak temperature areas in the waste canister

  3. Somatic cell nuclear transfer-derived embryonic stem cell lines in humans: pros and cons.

    Science.gov (United States)

    Langerova, Alena; Fulka, Helena; Fulka, Josef

    2013-12-01

    The recent paper, published by Mitalipov's group in Cell (Tachibana et al., 2013 ), reporting the production of human somatic cell nuclear transfer (SCNT) embryonic stem cells (ESCs), opens again the debate if, in the era of induced pluripotent stem cells (iPSCs), the production of these cells is indeed necessary and, if so, whether they are different from ESCs produced from spare embryos and iPSCs. It is our opinion that these questions are very difficult to answer because it is still unclear whether and how normal ESCs differ from iPSCs. PMID:24180743

  4. Blended learning concept for the support of know-how transfer in a nuclear company

    International Nuclear Information System (INIS)

    AREVA is the largest growing nuclear company. Development and construction of new power plants, sustainment and maintenance of existing installations require many professionally trained personnel, more than 800 new appointments per year trigger the in-plant training and education. The AREVA NP Training Center is in charge of the education and training of the personnel. The contribution deals with the applied blended learning concept and the know-how transfer within AREVA NP. Web-based training and the virtual class room are part of the concept.

  5. Meeting the manpower challenge in the transfer of nuclear power to developing countries

    International Nuclear Information System (INIS)

    One of the constraints to meeting projections of nuclear power's share of energy needs in the United States has been the various manpower problems, ranging from idling of construction manpower because of disruptions in the flow of materials and construction scheduling to the comptetition between various segments of the nuclear industry, government, and educational institutions for the available supply of experienced nuclear-oriented engineering personnel. Similar problems have been encountered in other countries which are presently partially dependent upon nuclear power for energy, and the competition for qualified personnel has become international in scope with considerable migrating of engineers among these nations. The global needs for staffing personnel of nuclear power plants and fuel cycle facilities to 1990 indicate continued stress on the international technical manpower supply. When considering the manpower and training necessary for the execution of a country's first nuclear project, the burden imposed on the purchaser's organization needs to be clearly understood. The work load and the responsibilities that cannot be transferred to other organizations is usually underestimated. In assessing the magnitude of the workload, four important points should be emphasized: (1) The responsibility for the ultimate success or failure of the nuclear project must be borne by the purchaser's organization. Contractors can assume responsibility for the performance of specific tasks but not for the entire project; (2) Even in a turnkey contact, the prime contractor normally will not assume financial responsibility for the performance of the local subcontractors, whose performance must be monitored and controlled by the purchaser's organization; (3) Many areas of a nuclear project involving domestic bureaucracy, politics, regulations, etc., can be dealt with effectively only by nationals. Examples of such areas are customs clearances and import licenses, visas, housing

  6. Knowledge Creation, Management and Transfer: The Experience of the Korea Institute of Nuclear Safety

    International Nuclear Information System (INIS)

    Regulatory activities are composed of four major functions: authorization, review and assessment, inspection and enforcement, and development of regulations and guidelines. To discharge these regulatory functions, the regulatory body needs to have an appropriate level of competencies rooted in sound knowledge and skills. These competencies can only be established by continuous efforts to absorb and digest the information available and to convert it into useful knowledge. As safety challenges arise from new reactor designs as well as from the ageing of nuclear power plants, the regulatory support organization (RSO)/technical support organization (TSO) should endeavour to develop the technical capacity to address such challenges. Knowledge should be managed to help individuals and groups to share valuable organizational insights and to transfer these insights from senior to junior staff in a systematic way. As an RSO/TSO in the Republic of Korea, the Korea Institute of Nuclear Safety (KINS) has been building up its competencies by creating, managing and transferring the knowledge necessary to perform regulatory functions. The paper describes how an RSO/TSO develops capacity building, taking the experience of KINS as an example. (author)

  7. Progress toward generating a ferret model of cystic fibrosis by somatic cell nuclear transfer

    Directory of Open Access Journals (Sweden)

    Engelhardt John F

    2003-11-01

    Full Text Available Abstract Mammalian cloning by nuclear transfer from somatic cells has created new opportunities to generate animal models of genetic diseases in species other than mice. Although genetic mouse models play a critical role in basic and applied research for numerous diseases, often mouse models do not adequately reproduce the human disease phenotype. Cystic fibrosis (CF is one such disease. Targeted ablation of the cystic fibrosis transmembrane conductance regulator (CFTR gene in mice does not adequately replicate spontaneous bacterial infections observed in the human CF lung. Hence, several laboratories are pursuing alternative animal models of CF in larger species such as the pig, sheep, rabbits, and ferrets. Our laboratory has focused on developing the ferret as a CF animal model. Over the past few years, we have investigated several experimental parameters required for gene targeting and nuclear transfer (NT cloning in the ferret using somatic cells. In this review, we will discuss our progress and the hurdles to NT cloning and gene-targeting that accompany efforts to generate animal models of genetic diseases in species such as the ferret.

  8. Aberrant Expression of Xist in Aborted Porcine Fetuses Derived from Somatic Cell Nuclear Transfer Embryos

    Directory of Open Access Journals (Sweden)

    Lin Yuan

    2014-11-01

    Full Text Available Cloned pigs generated by somatic cell nuclear transfer (SCNT show a greater ratio of early abortion during mid-gestation than normal controls. X-linked genes have been demonstrated to be important for the development of cloned embryos. To determine the relationship between the expression of X-linked genes and abortion of cloned porcine fetuses, the expression of X-linked genes were investigated by quantitative real-time polymerase chain reaction (q-PCR and the methylation status of Xist DMR was performed by bisulfate-specific PCR (BSP. q-PCR analysis indicated that there was aberrant expression of X-linked genes, especially the upregulated expression of Xist in both female and male aborted fetuses compared to control fetuses. Results of BSP suggested that hypomethylation of Xist occurred in aborted fetuses, whether male or female. These results suggest that the abnormal expression of Xist may be associated with the abortion of fetuses derived from somatic cell nuclear transfer embryos.

  9. Targeted disruption of Ataxia-telangiectasia mutated gene in miniature pigs by somatic cell nuclear transfer

    International Nuclear Information System (INIS)

    Highlights: • ATM gene-targeted pigs were produced by somatic cell nuclear transfer. • A novel large animal model for ataxia telangiectasia was developed. • The new model may provide an alternative to the mouse model. - Abstract: Ataxia telangiectasia (A-T) is a recessive autosomal disorder associated with pleiotropic phenotypes, including progressive cerebellar degeneration, gonad atrophy, and growth retardation. Even though A-T is known to be caused by the mutations in the Ataxia telangiectasia mutated (ATM) gene, the correlation between abnormal cellular physiology caused by ATM mutations and the multiple symptoms of A-T disease has not been clearly determined. None of the existing ATM mouse models properly reflects the extent to which neurological degeneration occurs in human. In an attempt to provide a large animal model for A-T, we produced gene-targeted pigs with mutations in the ATM gene by somatic cell nuclear transfer. The disrupted allele in the ATM gene of cloned piglets was confirmed via PCR and Southern blot analysis. The ATM gene-targeted pigs generated in the present study may provide an alternative to the current mouse model for the study of mechanisms underlying A-T disorder and for the development of new therapies

  10. Targeted disruption of Ataxia-telangiectasia mutated gene in miniature pigs by somatic cell nuclear transfer

    Energy Technology Data Exchange (ETDEWEB)

    Kim, Young June; Ahn, Kwang Sung; Kim, Minjeong; Kim, Min Ju; Park, Sang-Min; Ryu, Junghyun; Ahn, Jin Seop; Heo, Soon Young; Kang, Jee Hyun; Choi, You Jung [Department of Nanobiomedical Science and BK21 PLUS NBM Global Research Center for Regenerative Medicine, Dankook University, Cheonan (Korea, Republic of); Choi, Seong-Jun [Institute of Tissue Regeneration Engineering, Dankook University, Cheonan (Korea, Republic of); Shim, Hosup, E-mail: shim@dku.edu [Department of Nanobiomedical Science and BK21 PLUS NBM Global Research Center for Regenerative Medicine, Dankook University, Cheonan (Korea, Republic of); Institute of Tissue Regeneration Engineering, Dankook University, Cheonan (Korea, Republic of); Department of Physiology, Dankook University School of Medicine, Cheonan (Korea, Republic of)

    2014-10-03

    Highlights: • ATM gene-targeted pigs were produced by somatic cell nuclear transfer. • A novel large animal model for ataxia telangiectasia was developed. • The new model may provide an alternative to the mouse model. - Abstract: Ataxia telangiectasia (A-T) is a recessive autosomal disorder associated with pleiotropic phenotypes, including progressive cerebellar degeneration, gonad atrophy, and growth retardation. Even though A-T is known to be caused by the mutations in the Ataxia telangiectasia mutated (ATM) gene, the correlation between abnormal cellular physiology caused by ATM mutations and the multiple symptoms of A-T disease has not been clearly determined. None of the existing ATM mouse models properly reflects the extent to which neurological degeneration occurs in human. In an attempt to provide a large animal model for A-T, we produced gene-targeted pigs with mutations in the ATM gene by somatic cell nuclear transfer. The disrupted allele in the ATM gene of cloned piglets was confirmed via PCR and Southern blot analysis. The ATM gene-targeted pigs generated in the present study may provide an alternative to the current mouse model for the study of mechanisms underlying A-T disorder and for the development of new therapies.

  11. Cumulus-specific genes are transcriptionally silent following somatic cell nuclear transfer in a mouse model

    Institute of Scientific and Technical Information of China (English)

    2007-01-01

    This study investigated whether four cumulus-specific genes: follicular stimulating hormone receptor (FSHr), hyaluronan synthase 2 (Has2), prostaglandin synthase 2 (Ptgs2) and steroidogenic acute regulator protein (Star), were correctly reprogrammed to be transcriptionally silent following somatic cell nuclear transfer (SCNT) in a murine model. Cumulus cells of C57×CBA F1 female mouse were injected into enucleated oocytes, followed by activation in 10 μmol/L strontium chloride for 5 h and subsequent in vitro culture up to the blastocyst stage. Expression of cumulus-specific genes in SCNT-derived embryos at 2-cell, 4-cell and day 4.5 blastocyst stages was compared with corresponding in vivo fertilized embryos by real-time PCR. It was demonstrated that immediately after the first cell cycle, SCNT-derived 2-cell stage embryos did not express all four cumulus-specific genes, which continually remained silent at the 4-cell and blastocyst stages. It is therefore concluded that all four cumulus-specific genes were correctly reprogrammed to be silent following nuclear transfer with cumulus donor cells in the mouse model. This would imply that the poor preimplantation developmental competence of SCNT embryos derived from cumulus cells is due to incomplete reprogramming of other embryonic genes, rather than cumulus-specific genes.

  12. Interaction of bovine serum albumin (BSA) with novel gemini surfactants studied by synchrotron radiation scattering (SR-SAXS), circular dichroism (CD), and nuclear magnetic resonance (NMR).

    Science.gov (United States)

    Gospodarczyk, W; Szutkowski, K; Kozak, M

    2014-07-24

    The interaction of three dicationic (gemini) surfactants-3,3'-[1,6-(2,5-dioxahexane)]bis(1-dodecylimidazolium) chloride (oxyC2), 3,3'-[1,16-(2,15-dioxahexadecane)]bis(1-dodecylimidazolium) chloride (oxyC12), and 1,4-bis(butane)imidazole-1-yl-3-dodecylimidazolium chloride (C4)--with bovine serum albumin (BSA) has been studied by the use of small-angle X-ray scattering (SAXS), circular dichroism (CD), and (1)H nuclear magnetic resonance diffusometry. The results of CD studies show that the conformation of BSA was changed dramatically in the presence of all studied surfactants. The greater decrease (from 56 to 24%) in the α-helical structure of BSA was observed for oxyC2 surfactant. The radii of gyration estimated from SAXS data varied between 3 and 26 nm for the BSA/oxyC2 and BSA/oxyC12 systems. The hydrodynamic radius of the BSA/surfactant system estimated from NMR diffusometry varies between 5 and 11 nm for BSA/oxyC2 and 5 and 8 nm for BSA/oxyC12.

  13. N-doped carbon dots derived from bovine serum albumin and formic acid with one- and two-photon fluorescence for live cell nuclear imaging.

    Science.gov (United States)

    Tan, Mingqian; Li, Xintong; Wu, Hao; Wang, Beibei; Wu, Jing

    2015-12-01

    Carbon dots with both one- and two-photon fluorescence have drawn great attention for biomedical imaging. Herein, nitrogen-doped carbon dots were facilely developed by one-pot hydrothermal method using bovine serum albumin and formic acid as carbon sources. They are highly water-soluble with strong fluorescence when excited with ultraviolet or near infrared light. The carbon dots have a diameter of ~8.32 nm and can emit strong two-photon induced fluorescence upon excitation at 750 nm with a femtosecond laser. X-ray photoelectron spectrometer analysis revealed that the carbon dots contained three components, C, N and O, corresponding to the peak at 285, 398 and 532 eV, respectively. The Fourier-transform infrared spectroscopy analysis revealed that there are carboxyl and carboxylic groups on the surface, which allowed further linking of functional molecules. pH stability study demonstrated that the carbon dots are able to be used in a wide range of pH values. The fluorescence mechanism is also discussed in this study. Importantly, these carbon dots are biocompatible and highly photostable, which can be directly applied for both one- and two-photon living cell imaging. After proper surface functionalization with TAT peptide, they can be used as fluorescent probes for live cell nuclear-targeted imaging.

  14. The nuclear power industry's ageing workforce: Transfer of knowledge to the next generation

    International Nuclear Information System (INIS)

    This report is intended primarily for senior and middle level managers in nuclear power plant operating organizations. It is intended to provide them practical information they can use to improve the transfer of knowledge from the current generation of NPP operating organization personnel to the next generation in an effective manner. The information provided in this report is based upon the experience of Member State operating organizations as well as other related industries. In September 2000, the IAEA held a technical meeting on the topic of an ageing workforce and declining educational infrastructures. The proceedings of this meeting were distributed on CD-ROM as Working Material. Several recent IAEA meetings including a senior level meeting held in June 2002 in Vienna and a technical session of the IAEA General Conference in September 2002 addressed methods of knowledge transfer. This is the first IAEA report published on this specific topic. In 2000, the IAEA Technical Working Group on the Training and Qualification of NPP Personnel (TWG-T and Q) suggested that the IAEA should develop a publication on the definition of core competencies to be maintained by an NPP operating organization. The TWG-T and Q suggested that this TECDOC should provide additional detail beyond that specified in the recently revised Safety Guide NS-G-2.8, Recruitment, Qualification and Training of Personnel for Nuclear Power Plants. This task was included in the approved programme for 2002-2003. In March 2001, IAEA-TECDOC-1204, A Systematic Approach to Human Performance Improvement: Training Solutions was published. This TECDOC provides a comprehensive list of core competencies. These competencies provide the additional detail beyond that specified in Safety Guide NS-G-2.8 that the TWG-T and Q had suggested. The aspect of core competencies that is not addressed in IAEA-TECDOC-1204 is how to effectively transfer these competencies to the generation that replaces the workforce that

  15. Serial nuclear transfer improves the development of interspecies reconstructed giant panda (Aluropoda melanoleuca) embryos

    Institute of Scientific and Technical Information of China (English)

    2002-01-01

    Interspecies somatic nuclear transfer (NT) may provide a new approach for preservation of the endangered rare species. Previous interspecies cloning studies have shown that a nucleus from a quiescent somatic cell supports early development of reconstructed embryos in the ooplasm from another species. In this study, we transferred nonquiescent somatic cells from a giant panda into the perivitelline space of the enucleated rabbit oocytes. After electrofusion (at the rate of 71.6%) and electrical activation, 4.2% of the panda-rabbit reconstructed embryos developed to blastocyst in vitro. For improving the development rate of reconstructed embryos, we used serial NT in this study, I.e. Blastomeres from reconstructed morulae were transferred into the perivitelline space of the enucleated rabbit oocytes. The fusion rates in the groups of serial Ⅰ, serial Ⅱ and serial Ⅲ were 79.5%, 84.1% and 78.0%, respectively, having no difference with that of somatic group. And the blastocyst rates in serial NT groups were 19.4%, 13.5% and 10.3%, respectively, which are significantly higher than that in somatic NT group. These results indicate that the nuclei from nonquiescent somatic cells can support early development of reconstructed embryos and serial NT can improve the development rate of interspecies reconstructed embryos.

  16. Application of Direct Assessment Approaches and Methodologies to Cathodically Protected Nuclear Waste Transfer Lines

    International Nuclear Information System (INIS)

    The U.S. Department of Energy's (DOE) Hanford Site is responsible for the safe storage, retrieval, treatment, and disposal of approximately 54 million gallons (204 million liters) of radioactive waste generated since the site's inception in 1943. Today, the major structures involved in waste management at Hanford include 149 carbon steel single-shell tanks, 28 carbon-steel double-shell tanks, plus a network of buried metallic transfer lines and ancillary systems (pits, vaults, catch tanks, etc.) required to store, retrieve, and transfer waste within the tank farm system. Many of the waste management systems at Hanford are still in use today. In response to uncertainties regarding the structural integrity of these systems,' an independent, comprehensive integrity assessment of the Hanford Site piping system was performed. It was found that regulators do not require the cathodically protected pipelines located within the Hanford Site to be assessed by External Corrosion Direct Assessment (ECDA) or any other method used to ensure integrity. However, a case study is presented discussing the application of the direct assessment process on pipelines in such a nuclear environment. Assessment methodology and assessment results are contained herein. An approach is described for the monitoring, integration of outside data, and analysis of this information in order to identify whether coating deterioration accompanied by external corrosion is a threat for these waste transfer lines

  17. Application of Direct Assessment Approaches and Methodologies to Cathodically Protected Nuclear Waste Transfer Lines

    Energy Technology Data Exchange (ETDEWEB)

    Dahl, Megan M. [ARES Corporation, Richland, WA (United States); Pikas, Joseph [Schiff Associates, Sugar Land TX (United States); Edgemon, Glenn L. [ARES Corporation, Richland, WA (United States); Philo, Sarah [ARES Corporation, Richland, WA (United States)

    2013-01-22

    The U.S. Department of Energy's (DOE) Hanford Site is responsible for the safe storage, retrieval, treatment, and disposal of approximately 54 million gallons (204 million liters) of radioactive waste generated since the site's inception in 1943. Today, the major structures involved in waste management at Hanford include 149 carbon steel single-shell tanks, 28 carbon-steel double-shell tanks, plus a network of buried metallic transfer lines and ancillary systems (pits, vaults, catch tanks, etc.) required to store, retrieve, and transfer waste within the tank farm system. Many of the waste management systems at Hanford are still in use today. In response to uncertainties regarding the structural integrity of these systems,' an independent, comprehensive integrity assessment of the Hanford Site piping system was performed. It was found that regulators do not require the cathodically protected pipelines located within the Hanford Site to be assessed by External Corrosion Direct Assessment (ECDA) or any other method used to ensure integrity. However, a case study is presented discussing the application of the direct assessment process on pipelines in such a nuclear environment. Assessment methodology and assessment results are contained herein. An approach is described for the monitoring, integration of outside data, and analysis of this information in order to identify whether coating deterioration accompanied by external corrosion is a threat for these waste transfer lines.

  18. Heat Transfer Enhancement By Three-Dimensional Surface Roughness Technique In Nuclear Fuel Rod Bundles

    Science.gov (United States)

    Najeeb, Umair

    This thesis experimentally investigates the enhancement of single-phase heat transfer, frictional loss and pressure drop characteristics in a Single Heater Element Loop Tester (SHELT). The heater element simulates a single fuel rod for Pressurized Nuclear reactor. In this experimental investigation, the effect of the outer surface roughness of a simulated nuclear rod bundle was studied. The outer surface of a simulated fuel rod was created with a three-dimensional (Diamond-shaped blocks) surface roughness. The angle of corrugation for each diamond was 45 degrees. The length of each side of a diamond block is 1 mm. The depth of each diamond block was 0.3 mm. The pitch of the pattern was 1.614 mm. The simulated fuel rod had an outside diameter of 9.5 mm and wall thickness of 1.5 mm and was placed in a test-section made of 38.1 mm inner diameter, wall thickness 6.35 mm aluminum pipe. The Simulated fuel rod was made of Nickel 200 and Inconel 625 materials. The fuel rod was connected to 10 KW DC power supply. The Inconel 625 material of the rod with an electrical resistance of 32.3 kO was used to generate heat inside the test-section. The heat energy dissipated from the Inconel tube due to the flow of electrical current flows into the working fluid across the rod at constant heat flux conditions. The DI water was employed as working fluid for this experimental investigation. The temperature and pressure readings for both smooth and rough regions of the fuel rod were recorded and compared later to find enhancement in heat transfer coefficient and increment in the pressure drops. Tests were conducted for Reynold's Numbers ranging from 10e4 to 10e5. Enhancement in heat transfer coefficient at all Re was recorded. The maximum heat transfer co-efficient enhancement recorded was 86% at Re = 4.18e5. It was also observed that the pressure drop and friction factor increased by 14.7% due to the increased surface roughness.

  19. Development of buffalo (Bubalus bubalis embryonic stem cell lines from somatic cell nuclear transferred blastocysts

    Directory of Open Access Journals (Sweden)

    Syed Mohmad Shah

    2015-11-01

    Full Text Available We developed buffalo embryonic stem cell lines from somatic cell nuclear transfer derived blastocysts, produced by hand-guided cloning technique. The inner cell mass of the blastocyst was cut mechanically using a Microblade and cultured onto feeder cells in buffalo embryonic stem (ES cell culture medium at 38 °C in a 5% CO2 incubator. The stem cell colonies were characterized for alkaline phosphatase activity, karyotype, pluripotency and self-renewal markers like OCT4, NANOG, SOX2, c-Myc, FOXD3, SSEA-1, SSEA-4, TRA-1-60, TRA-1-81 and CD90. The cell lines also possessed the capability to differentiate across all the three germ layers under spontaneous differentiation conditions.

  20. Modeling Electronic-Nuclear Interactions for Excitation Energy Transfer Processes in Light-Harvesting Complexes.

    Science.gov (United States)

    Lee, Mi Kyung; Coker, David F

    2016-08-18

    An accurate approach for computing intermolecular and intrachromophore contributions to spectral densities to describe the electronic-nuclear interactions relevant for modeling excitation energy transfer processes in light harvesting systems is presented. The approach is based on molecular dynamics (MD) calculations of classical correlation functions of long-range contributions to excitation energy fluctuations and a separate harmonic analysis and single-point gradient quantum calculations for electron-intrachromophore vibrational couplings. A simple model is also presented that enables detailed analysis of the shortcomings of standard MD-based excitation energy fluctuation correlation function approaches. The method introduced here avoids these problems, and its reliability is demonstrated in accurate predictions for bacteriochlorophyll molecules in the Fenna-Matthews-Olson pigment-protein complex, where excellent agreement with experimental spectral densities is found. This efficient approach can provide instantaneous spectral densities for treating the influence of fluctuations in environmental dissipation on fast electronic relaxation. PMID:27472379

  1. Genomic stability of lyophilized sheep somatic cells before and after nuclear transfer.

    Directory of Open Access Journals (Sweden)

    Domenico Iuso

    Full Text Available The unprecedented decline of biodiversity worldwide is urging scientists to collect and store biological material from seriously threatened animals, including large mammals. Lyophilization is being explored as a low-cost system for storage in bio-banks of cells that might be used to expand or restore endangered or extinct species through the procedure of Somatic Cell Nuclear Transfer (SCNT. Here we report that the genome is intact in about 60% of lyophylized sheep lymphocytes, whereas DNA damage occurs randomly in the remaining 40%. Remarkably, lyophilized nuclei injected into enucleated oocytes are repaired by a robust DNA repairing activity of the oocytes, and show normal developmental competence. Cloned embryos derived from lyophylized cells exhibited chromosome and cellular composition comparable to those of embryos derived from fresh donor cells. These findings support the feasibility of lyophylization as a storage procedure of mammalian cells to be used for SCNT.

  2. Live embryo imaging to follow cell cycle and chromosomes stability after nuclear transfer.

    Science.gov (United States)

    Balbach, Sebastian T; Boiani, Michele

    2015-01-01

    Nuclear transfer (NT) into mouse oocytes yields a transcriptionally and functionally heterogeneous population of cloned embryos. Most studies of NT embryos consider only embryos at predefined key stages (e.g., morula or blastocyst), that is, after the bulk of reprogramming has taken place. These retrospective approaches are of limited use to elucidate mechanisms of reprogramming and to predict developmental success. Observing cloned embryo development using live embryo cinematography has the potential to reveal otherwise undetectable embryo features. However, light exposure necessary for live cell cinematography is highly toxic to cloned embryos. Here we describe a protocol for combined bright-field and fluorescence live-cell imaging of histone H2b-GFP expressing mouse embryos, to record cell divisions up to the blastocyst stage. This protocol, which can be adapted to observe other reporters such as Oct4-GFP or Nanog-GFP, allowed us to quantitatively analyze cleavage kinetics of cloned embryos. PMID:25287344

  3. Modeling Electronic-Nuclear Interactions for Excitation Energy Transfer Processes in Light-Harvesting Complexes.

    Science.gov (United States)

    Lee, Mi Kyung; Coker, David F

    2016-08-18

    An accurate approach for computing intermolecular and intrachromophore contributions to spectral densities to describe the electronic-nuclear interactions relevant for modeling excitation energy transfer processes in light harvesting systems is presented. The approach is based on molecular dynamics (MD) calculations of classical correlation functions of long-range contributions to excitation energy fluctuations and a separate harmonic analysis and single-point gradient quantum calculations for electron-intrachromophore vibrational couplings. A simple model is also presented that enables detailed analysis of the shortcomings of standard MD-based excitation energy fluctuation correlation function approaches. The method introduced here avoids these problems, and its reliability is demonstrated in accurate predictions for bacteriochlorophyll molecules in the Fenna-Matthews-Olson pigment-protein complex, where excellent agreement with experimental spectral densities is found. This efficient approach can provide instantaneous spectral densities for treating the influence of fluctuations in environmental dissipation on fast electronic relaxation.

  4. Dependence of the complete set of spin transfer coefficients on effective interaction in nuclear medium

    International Nuclear Information System (INIS)

    Multistep direct reactions 40Ca(p, p'x) at 392 MeV and 40Ca(p, nx) at 346 MeV are analyzed including up to three-step process. The double differential inclusive cross sections and the complete set of spin transfer coefficients Dij are calculated by the semiclassical distorted wave model and compared with experimental data. We use single particle wave functions in a Woods-Saxon potential incorporating the Wigner transform of a one-body density matrix and also introduce a phenomenological effective mass m* of a nucleon in the target. Analysis of Dij in terms of an effective interaction in nuclear medium is also done. (author)

  5. Potential of primary kidney cells for somatic cell nuclear transfer mediated transgenesis in pig

    Directory of Open Access Journals (Sweden)

    Richter Anne

    2012-11-01

    Full Text Available Abstract Background Somatic cell nuclear transfer (SCNT is currently the most efficient and precise method to generate genetically tailored pig models for biomedical research. However, the efficiency of this approach is crucially dependent on the source of nuclear donor cells. In this study, we evaluate the potential of primary porcine kidney cells (PKCs as cell source for SCNT, including their proliferation capacity, transfection efficiency, and capacity to support full term development of SCNT embryos after additive gene transfer or homologous recombination. Results PKCs could be maintained in culture with stable karyotype for up to 71 passages, whereas porcine fetal fibroblasts (PFFs and porcine ear fibroblasts (PEFs could be hardly passaged more than 20 times. Compared with PFFs and PEFs, PKCs exhibited a higher proliferation rate and resulted in a 2-fold higher blastocyst rate after SCNT and in vitro cultivation. Among the four transfection methods tested with a GFP expression plasmid, best results were obtained with the NucleofectorTM technology, resulting in transfection efficiencies of 70% to 89% with high fluorescence intensity, low cytotoxicity, good cell proliferation, and almost no morphological signs of cell stress. Usage of genetically modified PKCs in SCNT resulted in approximately 150 piglets carrying at least one of 18 different transgenes. Several of those pigs originated from PKCs that underwent homologous recombination and antibiotic selection before SCNT. Conclusion The high proliferation capacity of PKCs facilitates the introduction of precise and complex genetic modifications in vitro. PKCs are thus a valuable cell source for the generation of porcine biomedical models by SCNT.

  6. Spent Nuclear Fuel Dry Transfer System Cold Demonstration Project Final Report

    Energy Technology Data Exchange (ETDEWEB)

    Christensen, Max R; McKinnon, M. A.

    1999-12-01

    The spent nuclear fuel dry transfer system (DTS) provides an interface between large and small casks and between storage-only and transportation casks. It permits decommissioning of reactor pools after shutdown and allows the use of large storage-only casks for temporary onsite storage of spent nuclear fuel irrespective of reactor or fuel handling limitations at a reactor site. A cold demonstration of the DTS prototype was initiated in August 1996 at the Idaho National Engineering and Environmental Laboratory (INEEL). The major components demonstrated included the fuel assembly handling subsystem, the shield plug/lid handling subsystem, the cask interface subsystem, the demonstration control subsystem, a support frame, and a closed circuit television and lighting system. The demonstration included a complete series of DTS operations from source cask receipt and opening through fuel transfer and closure of the receiving cask. The demonstration included both normal operations and recovery from off-normal events. It was designed to challenge the system to determine whether there were any activities that could be made to jeopardize the activities of another function or its safety. All known interlocks were challenged. The equipment ran smoothly and functioned as designed. A few "bugs" were corrected. Prior to completion of the demonstration testing, a number of DTS prototype systems were modified to apply lessons learned to date. Additional testing was performed to validate the modifications. In general, all the equipment worked exceptionally well. The demonstration also helped confirm cost estimates that had been made at several points in the development of the system.

  7. Transfer

    DEFF Research Database (Denmark)

    Wahlgren, Bjarne; Aarkrog, Vibe

    Bogen er den første samlede indføring i transfer på dansk. Transfer kan anvendes som praksis-filosofikum. Den giver en systematisk indsigt til den studerende, der spørger: Hvordan kan teoretisk viden bruges til at reflektere over handlinger i situationer, der passer til min fremtidige arbejdsplads?...

  8. Knowledge transfer in Swedish Nuclear Power Plants in connection with retirements; Kompetensoeverfoering paa svenska kaernkraftverk i samband med pensionsavgaangar

    Energy Technology Data Exchange (ETDEWEB)

    Larsson, Annika; Ohlsson, Kjell; Roos, Anna

    2007-12-13

    This report displays how the Swedish nuclear power plants Forsmark, Oskarshamn and Ringhals work with knowledge management. The report also consists of a literature review of appropriate ways to extract tacit knowledge as well as methods to transfer competence. The report is made up of a smaller number of interviews at the nuclear power plants in combination with a questionnaire distributed to a larger number of people at the plants. The results of the interview study is that only one of the Swedish nuclear power plants have a programme to transfer knowledge from older staff to newer. This is, however, not a programme for everyone. Another plant has a programme for knowledge building, but only for their specialists. At both plants, which lack a programme, the interviewees request more structure in knowledge transfer; even though they feel the current way of transferring knowledge with mentors works well. Besides more structure, interviewees present a wish to have more time for knowledge transfer as well as the opportunity to recruit more than needed. Recruiting more than needed is however not very simple due to multiple causes such as nominal sizing departments and a difficulty of recruiting people to work far from larger cities. The way things are now, many feel too under-staffed and under a lot of time pressure daily to also have time for knowledge transfer besides their normal work.

  9. Phenotypes of Aging Postovulatory Oocytes After Somatic Cell Nuclear Transfer in Mice.

    Science.gov (United States)

    Lee, Ah Reum; Shimoike, Takashi; Wakayama, Teruhiko; Kishigami, Satoshi

    2016-06-01

    Oocytes rapidly lose their developmental potential after ovulation, termed postovulatory oocyte aging, and often exhibit characteristic phenotypes, such as cytofragmentation, abnormal spindle shapes, and chromosome misalignments. Here, we reconstructed mouse oocytes using somatic cell nuclear transfer (SCNT) to reveal the effect of somatic cell-derived nuclei on oocyte physiology during aging. Normal oocytes started undergoing cytofragmentation 24 hours after oocyte collection; however, this occurred earlier in SCNT oocytes and was more severe at 48 hours, suggesting that the transferred somatic cell nuclei affected oocyte physiology. We found no difference in the status of acetylated α-tubulin (Ac-Tub) and α-tubulin (Tub) between normal and SCNT aging oocytes, but unlike normal oocytes, aging SCNT oocytes did not have astral microtubules. Interestingly, aging SCNT oocytes displayed more severely scattered chromosomes or irregularly shaped spindles. Observations of the microfilaments showed that, in normal oocytes, there was a clear actin ring beneath the plasma membrane and condensed microfilaments around the spindle (the actin cap) at 0 hours, and the actin filaments started degenerating at 1 hour, becoming completely disrupted and distributed to the cytoplasm at 24 hours. By contrast, in SCNT oocytes, an actin cap formed around the transplanted nuclei within 1 hour of SCNT, which was still present at 24 hours. Thus, SCNT oocytes age in a similar but distinct way, suggesting that they not only contain nuclei with abnormal epigenetics but are also physiologically different. PMID:27253626

  10. Cloned pigs derived from somatic cell nuclear transfer embryos cultured in vitro at low oxygen tension

    Institute of Scientific and Technical Information of China (English)

    2006-01-01

    Pig cloning has great potential to human xenotransplantation. The present study was designed to establish a more efficient system for producing cloned pigs by somatic cell nuclear transfer (SCNT). Our approach was as follows: SCNT embryos were reconstructed by using fetal fibroblasts of Chinese miniature pig as donors and in vitro matured oocytes of prepubertal gilts as recipients. Reconstructed embryos were induced by electrical fusion/activation and cultured in BSA-containing North Carolina State University 23 medium (NCSU-23) or Porcine Zygote Medium (PZM-3) at the gas condition of 5% CO2, 7% O2, 88% N2. A total of 230 cloned embryos were transferred to three surrogate sows, producing three piglets. One of them is apparently healthy. The clonal provenance of the piglet was indicated by its coat color and confirmed by DNA microsatellite analysis. These results indicate that the use of in vitro matured oocytes from prepubertal gilts as recipient, combined with cloned embryos cultured at low oxygen tension is an effective way to produce cloned pigs.

  11. Inheritance of mitochondrial DNA in serially recloned pigs by somatic cell nuclear transfer (SCNT)

    Energy Technology Data Exchange (ETDEWEB)

    Do, Minhwa; Jang, Won-Gu; Hwang, Jeong Hee; Jang, Hoon; Kim, Eun-Jung; Jeong, Eun-Jeong [Regenerative Medicine Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon 305 806 (Korea, Republic of); Shim, Hosup [Department of Physiology, Dankook University School of Medicine, Cheonan 330 714 (Korea, Republic of); Hwang, Sung Soo; Oh, Keon Bong; Byun, Sung June [Animal Biotechnology Division, National Institute of Animal Science, Rural Development Administration, Suwon (Korea, Republic of); Kim, Jin-Hoi [Department of Animal Biotechnology, Konkuk University, Seoul 143 701 (Korea, Republic of); Lee, Jeong Woong, E-mail: jwlee@kribb.re.kr [Regenerative Medicine Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon 305 806 (Korea, Republic of)

    2012-08-10

    Highlights: Black-Right-Pointing-Pointer We success serial SCNT through the third generation using pig fibroblasts. Black-Right-Pointing-Pointer Donor-specific mtDNA in the recloned pigs was detected. Black-Right-Pointing-Pointer SCNT affect mtDNA mounts. -- Abstract: Somatic cell nuclear transfer (SCNT) has been established for the transmission of specific nuclear DNA. However, the fate of donor mitochondrial DNA (mtDNA) remains unclear. Here, we examined the fate of donor mtDNA in recloned pigs through third generations. Fibroblasts of recloned pigs were obtained from offspring of each generation produced by fusion of cultured fibroblasts from a Minnesota miniature pig (MMP) into enucleated oocytes of a Landrace pig. The D-loop regions from the mtDNA of donor and recipient differ at nucleotide sequence positions 16050 (A{yields}T), 16062 (T{yields}C), and 16135 (G{yields}A). In order to determine the fate of donor mtDNA in recloned pigs, we analyzed the D-loop region of the donor's mtDNA by allele-specific PCR (AS-PCR) and real-time PCR. Donor mtDNA was successfully detected in all recloned offspring (F1, F2, and F3). These results indicate that heteroplasmy that originate from donor and recipient mtDNA is maintained in recloned pigs, resulting from SCNT, unlike natural reproduction.

  12. Transfer-induced fission in inverse kinematics: Impact on experimental and evaluated nuclear data bases

    International Nuclear Information System (INIS)

    Inverse kinematics is a new tool to study nuclear fission. Its main advantage is the possibility to measure with an unmatched resolution the atomic number of fission fragments, leading to new observables in the properties of fission-fragment distributions. In addition to the resolution improvement, the study of fission based on nuclear collisions in inverse kinematics beneficiates from a larger view with respect to the neutron-induced fission, as in a single experiment the number of fissioning systems and the excitation energy range are widden. With the use of spectrometers, mass and kinetic-energy distributions may now be investigated as a function of the proton and neutron number sharing. The production of fissioning nuclei in transfer reactions allows studying the isotopic yields of fission fragments as a function of the excitation energy. The higher excitation energy resulting in the fusion reaction leading to the compound nucleus 250Cf at an excitation energy of 45MeV is also presented. With the use of inverse kinematics, the charge polarisation of fragments at scission is now revealed with high precision, and it is shown that it cannot be neglected, even at higher excitation energies. In addition, the kinematical properties of the fragments inform on the deformation configuration at scission. (orig.)

  13. Multi-Scale-Structured Composite Coatings by Plasma-Transferred Arc for Nuclear Applications

    Science.gov (United States)

    Werry, A.; Chazelas, C.; Denoirjean, A.; Valette, S.; Vardelle, A.; Meillot, E.

    2016-01-01

    In nuclear plants, the replacement of hardfacing Stellite, a cobalt-based alloy, on parts of the piping system in connection with the reactor has been investigated since the late 60's. Various Fe-based or Ni-based alloys, Co-free or with a low content of Co, have been developed but with mechanical properties generally lower than that of Stellites. The 4th generation nuclear plants impose additional or more stringent requirements for hardfacing materials. Plasma-transferred arc (PTA) coatings of cobalt-free nickel-based alloys with the addition of sub-micrometric or micrometric alumina particles are thought to be a potential solution for tribological applications in the primary system of sodium-cooled fast reactors. In this study, PTA coatings of nickel-based alloys reinforced with alumina particles were deposited on 316L stainless steel substrates. Under the conditions of this study, the addition of alumina particles resulted in a refinement of coating microstructure and the improvement of their resistance to abrasive wear. However, it does not bring about any change in coating micro-hardness.

  14. Transfer-induced fission in inverse kinematics: Impact on experimental and evaluated nuclear data bases

    Science.gov (United States)

    Farget, F.; Caamaño, M.; Ramos, D.; Rodrıguez-Tajes, C.; Schmidt, K.-H.; Audouin, L.; Benlliure, J.; Casarejos, E.; Clément, E.; Cortina, D.; Delaune, O.; Derkx, X.; Dijon, A.; Doré, D.; Fernández-Domınguez, B.; Gaudefroy, L.; Golabek, C.; Heinz, A.; Jurado, B.; Lemasson, A.; Paradela, C.; Roger, T.; Salsac, M. D.; Schmitt, C.

    2015-12-01

    Inverse kinematics is a new tool to study nuclear fission. Its main advantage is the possibility to measure with an unmatched resolution the atomic number of fission fragments, leading to new observables in the properties of fission-fragment distributions. In addition to the resolution improvement, the study of fission based on nuclear collisions in inverse kinematics beneficiates from a larger view with respect to the neutron-induced fission, as in a single experiment the number of fissioning systems and the excitation energy range are widden. With the use of spectrometers, mass and kinetic-energy distributions may now be investigated as a function of the proton and neutron number sharing. The production of fissioning nuclei in transfer reactions allows studying the isotopic yields of fission fragments as a function of the excitation energy. The higher excitation energy resulting in the fusion reaction leading to the compound nucleus 250Cf at an excitation energy of 45MeV is also presented. With the use of inverse kinematics, the charge polarisation of fragments at scission is now revealed with high precision, and it is shown that it cannot be neglected, even at higher excitation energies. In addition, the kinematical properties of the fragments inform on the deformation configuration at scission.

  15. Longitudinal study of reproductive performance of female cattle produced by somatic cell nuclear transfer.

    Directory of Open Access Journals (Sweden)

    Irina A Polejaeva

    Full Text Available The objective of this study was to determine whether or not reproductive performance in cattle produced by somatic cell nuclear transfer (SCNT is significantly different from that of their genetic donors. To address this question, we directed two longitudinal studies using different embryo production procedures: (1 superovulation followed by artificial insemination (AI and embryo collection and (2 ultrasound-guided ovum pick-up followed by in vitro fertilization (OPU-IVF. Collectively, these two studies represent the largest data set available for any species on the reproductive performance of female clones and their genetic donors as measured by their embryo production outcomes in commercial embryo production program. The large-scale study described herein was conducted over a six-year period of time and provides a unique comparison of 96 clones to the 40 corresponding genetic donors. To our knowledge, this is the first longitudinal study on the reproductive performance of cattle clones using OPU-IVF. With nearly 2,000 reproductive procedures performed and more than 9,200 transferable embryos produced, our observations show that the reproductive performance of cattle produced by SCNT is not different compared to their genetic donors for the production of transferable embryos after either AI followed by embryo collection (P = 0.77 or OPU-IVF (P = 0.97. These data are in agreement with previous reports showing that the reproductive capabilities of cloned cattle are equal to that of conventionally produced cattle. In conclusion, results of this longitudinal study once again demonstrate that cloning technology, in combination with superovulation, AI and embryo collection or OPU-IVF, provides a valuable tool for faster dissemination of superior maternal genetics.

  16. Heat Transfer in Waste Glass Melts - Measurement and Implications for Nuclear Waste Vitrification

    Science.gov (United States)

    Wang, Chuan

    Thermal properties of waste glass melts, such as high temperature density and thermal conductivity, are relevant to heat transfer processes in nuclear waste vitrification. Experimental measurement techniques were developed and applied to four nuclear waste glasses representative of those currently projected for treatment of Hanford HLW and LAW streams to study heat flow mechanisms in nuclear waste vitrification. Density measurement results by Archimedes' method indicated that densities of the melts investigated varied considerably with composition and temperature. Thermal diffusivities of waste melts were determined at nominal melter operating temperatures using a temperature-wave technique. Thermal conductivities were obtained by combining diffusivity data with the experimentally-acquired densities of the melts and their known heat capacities. The experimental results display quite large positive dependences of conductivities on temperature for some samples and much weaker positive temperature dependences for others. More importantly, there is observed a big change in the slopes of the conductivities versus temperature as temperature is increased for two of the melts, but not for the other two. This behavior was interpreted in terms of the changing contributions of radiation and conduction with temperature and composition dependence of the absorption coefficient. Based on the obtained thermal conductivities, a simple model for a waste glass melter was set up, which was used to analyze the relative contributions of conduction and radiation individually and collectively to the overall heat flow and to investigate factors and conditions that influence the radiation contribution to heat flow. The modeling results showed that unlike the case at lower temperatures, the radiant energy flow through waste melts could be predominant compared with conduction at temperature of about 900 °C or higher. However, heat flow due to radiation was roughly equal to that from

  17. Finite-thrust optimization of interplanetary transfers of space vehicle with bimodal nuclear thermal propulsion

    Science.gov (United States)

    Kharytonov, Oleksii M.; Kiforenko, Boris M.

    2011-08-01

    The nuclear thermal rocket (NTR) propulsion is one of the leading promising technologies for primary space propulsion for manned exploration of the solar system due to its high specific impulse capability and sufficiently high thrust-to-weight ratio. Another benefit of NTR is its possible bimodal design, when nuclear reactor is used for generation of a jet thrust in a high-thrust mode and (with an appropriate power conversion system) as a source of electric power to supply the payload and the electric engines in a low-thrust mode. The model of the NTR thrust control was developed considering high-thrust NTR as a propulsion system of limited power and exhaust velocity. For the proposed model the control of the thrust value is accomplished by the regulation of reactor thermal power and propellant mass flow rate. The problem of joint optimization of the combination of high- and low-thrust arcs and the parameters of bimodal NTR (BNTR) propulsion system is considered for the interplanetary transfers. The interplanetary trajectory of the space vehicle is formed by the high-thrust NTR burns, which define planet-centric maneuvers and by the low-thrust heliocentric arcs where the nuclear electric propulsion (NEP) is used. The high-thrust arcs are analyzed using finite-thrust approach. The motion of the corresponding dynamical system is realized in three phase spaces concerning the departure planet-centric maneuver by means of high-thrust NTR propulsion, the low-thrust NEP heliocentric maneuver and the approach high-thrust NTR planet-centric maneuver. The phase coordinates are related at the time instants of the change of the phase spaces due to the relations between the space vehicle masses. The optimal control analysis is performed using Pontryagin's maximum principle. The numerical results are analyzed for Earth-Mars "sprint" transfer. The optimal values of the parameters that define the masses of NTR and NEP subsystems have been evaluated. It is shown that the low

  18. Activation of ribosomal RNA genes in porcine embryos produced in vitro or by somatic cell nuclear transfer

    DEFF Research Database (Denmark)

    Bjerregaard, Bolette; Pedersen, Hanne Gervi; Jakobsen, Anne Sørig;

    2007-01-01

    nuclear transfer (SCNT) using fluorescence in situ hybridization (FISH) with an rDNA probe and subsequent visualization of the nucleolar proteins by silver staining. In the 205 IVP embryos investigated, all two-cell embryos (n = 34) were categorized as transcriptionally inactive. At the late four...

  19. Mitochondrial DNA heteroplasmy in ovine fetuses and sheep cloned by somatic cell nuclear transfer

    Directory of Open Access Journals (Sweden)

    Müller Mathias

    2007-12-01

    Full Text Available Abstract Background The mitochondrial DNA (mtDNA of the cloned sheep "Dolly" and nine other ovine clones produced by somatic cell nuclear transfer (SCNT was reported to consist only of recipient oocyte mtDNA without any detectable mtDNA contribution from the nucleus donor cell. In cattle, mouse and pig several or most of the clones showed transmission of nuclear donor mtDNA resulting in mitochondrial heteroplasmy. To clarify the discrepant transmission pattern of donor mtDNA in sheep clones we analysed the mtDNA composition of seven fetuses and five lambs cloned from fetal fibroblasts. Results The three fetal fibroblast donor cells used for SCNT harboured low mtDNA copy numbers per cell (A: 753 ± 54, B: 292 ± 33 and C: 561 ± 88. The ratio of donor to recipient oocyte mtDNAs was determined using a quantitative amplification refractory mutation system (ARMS PCR (i.e. ARMS-qPCR. For quantification of SNP variants with frequencies below 0.1% we developed a restriction endonuclease-mediated selective quantitative PCR (REMS-qPCR. We report the first cases (n = 4 fetuses, n = 3 lambs of recipient oocyte/nuclear donor mtDNA heteroplasmy in SCNT-derived ovine clones demonstrating that there is no species-effect hindering ovine nucleus-donor mtDNA from being transmitted to the somatic clonal offspring. Most of the heteroplasmic clones exhibited low-level heteroplasmy (0.1% to 0.9%, n = 6 indicating neutral transmission of parental mtDNAs. High-level heteroplasmy (6.8% to 46.5% was observed in one case. This clone possessed a divergent recipient oocyte-derived mtDNA genotype with three rare amino acid changes compared to the donor including one substitution at an evolutionary conserved site. Conclusion Our study using state-of-the-art techniques for mtDNA quantification, like ARMS-qPCR and the novel REMS-qPCR, documents for the first time the transmission of donor mtDNA into somatic sheep clones. MtDNA heteroplasmy was detected in seven of 12 clones

  20. The role of tacit knowledge and the challenges in transferring it in the nuclear power plant context

    International Nuclear Information System (INIS)

    Full text: In nuclear power plant context all the relevant knowledge should be in explicit (written) form and the documentation is controlled by legislation and official regulations. However, knowledge has also a tacit element, which is the know-how of individuals including mental models, crafts, skills, intuitions, hunches and feelings which may be very difficult or even impossible to articulate. In this preliminary study, the aim is to find out the role of tacit knowledge in the nuclear power plant context and the challenges related to it at the moment. Furthermore, the current methods and practices in use for transferring the tacit knowledge in power plants will also be examined. The study is conducted in the two Finnish nuclear power plants, in Olkiluoto and Loviisa. The data are collected in March and April 2004 by interviewing 8-10 key informants at each plant. The interviewees are mainly middle and top management with a broad and deep view on the subject. The study is planned to be followed by a 2-3 year project, in which the transfer of tacit knowledge will be examined in four cases, in which the role and transfer of tacit knowledge has been found critical. In these cases, methods for improving the transfer of tacit knowledge will be developed, piloted and evaluated. Based on the experiences from the cases, the methods will be finally applied also to other parts of the organizations. As a result of the data gathered so far, even though the emphasis of the knowledge in the nuclear power plant context was considered to be on explicit knowledge, also the role of tacit knowledge was considered important. The role of tacit knowledge was especially emphasized in knowledge related to e.g. the building process of the power plant (e.g. design bases and project know-how); the experience of using the power plants; finding the relevant explicit knowledge from the paper files; interpreting and evaluating the resonance of issues and situations; the know-how embedded in

  1. Coupled quantum-classical method for long range charge transfer: relevance of the nuclear motion to the quantum electron dynamics

    International Nuclear Information System (INIS)

    Charge and excitonic-energy transfer phenomena are fundamental for energy conversion in solar cells as well as artificial photosynthesis. Currently, much interest is being paid to light-harvesting and energy transduction processes in supramolecular structures, where nuclear dynamics has a major influence on electronic quantum dynamics. For this reason, the simulation of long range electron transfer in supramolecular structures, under environmental conditions described within an atomistic framework, has been a difficult problem to study. This work describes a coupled quantum mechanics/molecular mechanics method that aims at describing long range charge transfer processes in supramolecular systems, taking into account the atomistic details of large molecular structures, the underlying nuclear motion, and environmental effects. The method is applied to investigate the relevance of electron–nuclei interaction on the mechanisms for photo-induced electron–hole pair separation in dye-sensitized interfaces as well as electronic dynamics in molecular structures. (paper)

  2. Coupled quantum-classical method for long range charge transfer: relevance of the nuclear motion to the quantum electron dynamics.

    Science.gov (United States)

    da Silva, Robson; Hoff, Diego A; Rego, Luis G C

    2015-04-10

    Charge and excitonic-energy transfer phenomena are fundamental for energy conversion in solar cells as well as artificial photosynthesis. Currently, much interest is being paid to light-harvesting and energy transduction processes in supramolecular structures, where nuclear dynamics has a major influence on electronic quantum dynamics. For this reason, the simulation of long range electron transfer in supramolecular structures, under environmental conditions described within an atomistic framework, has been a difficult problem to study. This work describes a coupled quantum mechanics/molecular mechanics method that aims at describing long range charge transfer processes in supramolecular systems, taking into account the atomistic details of large molecular structures, the underlying nuclear motion, and environmental effects. The method is applied to investigate the relevance of electron-nuclei interaction on the mechanisms for photo-induced electron-hole pair separation in dye-sensitized interfaces as well as electronic dynamics in molecular structures. PMID:25767107

  3. Assessment of nuclear transfer techniques to prevent the transmission of heritable mitochondrial disorders without compromising embryonic development competence in mice.

    Science.gov (United States)

    Neupane, Jitesh; Vandewoestyne, Mado; Ghimire, Sabitri; Lu, Yuechao; Qian, Chen; Van Coster, Rudy; Gerris, Jan; Deroo, Tom; Deforce, Dieter; De Sutter, Petra; Heindryckx, Björn

    2014-09-01

    To evaluate and compare mitochondrial DNA (mtDNA) carry-over and embryonic development potential between different nuclear transfer techniques we performed germinal vesicle nuclear transfer (GV NT), metaphase-II spindle-chromosome-complex (MII-SCC) transfer and pronuclear transfer (PNT) in mice. No detectable mtDNA carry-over was seen in most of the reconstructed oocytes and embryos. No significant differences were seen in mtDNA carry-over rate between GV NT (n=20), MII-SCC transfer (0.29 ± 0.63; n=21) and PNT (0.29 ± 0.75; n=25). Blastocyst formation was not compromised after either PNT (88%; n=18) or MII-SCC transfer (86%; n=27). Further analysis of blastomeres from cleaving embryos (n=8) demonstrated undetectable mtDNA carry-over in all but one blastomere. We show that NT in the germ line is potent to prevent transmission of heritable mtDNA disorders with the applicability for patients attempting reproduction.

  4. Analysis on the revision of the United States authorizing procedure for the transfer of unclassified nuclear technology

    Energy Technology Data Exchange (ETDEWEB)

    Yoon, Sung-ho; Seo, Hana; Lee, Chansuh; Kim, Jong-sook [Korea Institute of Nuclear Nonproliferation and Control, Daejeon (Korea, Republic of)

    2015-10-15

    The DOE (Department Of Energy) has not comprehensively update 10CFR810 since 1986. Since then, the global civil nuclear market has expanded, particularly in China, the Middle East, and Eastern Europe, with vendors from France, Japan, the Republic of Korea, Russia, and Canada. In result, DOE issued revised 810 in respond to comments received from the public and commercial nuclear market changes. This regulation revision improves the efficiency of authorization process to promote national nuclear industry while maintaining nonproliferation control. Even though ROK has initiated a legal basis for Intangible technology transfer (ITT) for nuclear export control, working implementation system is not set up. This research proposes recommendable ITT implementation of the ROK according to the analysis result of the US regulation. In this revision, of 124 countries had been classified as general authorization under 10CFR810, 80 countries reclassified into the specific authorization. By remaining 'fast track' for specific authorization, in particular, time frames for internal DOE and interagency reviews are reduced. This means the US government actively copes with commercial nuclear market expands to promote their industry. Meanwhile, by remaining some of nuclear-weapon states (China, Russia, India) as specific authorization maintaining that the determinations are consistent with current US national security, diplomatic, and trade policy. By benchmarking the US regulation, Korea can improve the efficiency of the technology transfer authorization process easing the regulatory burden by reducing uncertainty and timelines while maintaining the highest level of nonproliferation control.

  5. Effects of Histone Deacetylase Inhibitor Oxamflatin on In Vitro Porcine Somatic Cell Nuclear Transfer Embryos

    Science.gov (United States)

    Hou, Liming; Ma, Fanhua; Yang, Jinzeng; Riaz, Hasan; Wang, Yongliang; Wu, Wangjun; Xia, Xiaoliang; Ma, Zhiyuan; Zhou, Ying; Zhang, Lin; Ying, Wenqin; Xu, Dequan; Zuo, Bo; Ren, Zhuqing

    2014-01-01

    Abstract Low cloning efficiency is considered to be caused by the incomplete or aberrant epigenetic reprogramming of differentiated donor cells in somatic cell nuclear transfer (SCNT) embryos. Oxamflatin, a novel class of histone deacetylase inhibitor (HDACi), has been found to improve the in vitro and full-term developmental potential of SCNT embryos. In the present study, we studied the effects of oxamflatin treatment on in vitro porcine SCNT embryos. Our results indicated that the rate of in vitro blastocyst formation of SCNT embryos treated with 1 μM oxamflatin for 15 h postactivation was significantly higher than all other treatments. Treatment of oxamflatin decreased the relative histone deacetylase (HDAC) activity in cloned embryos and resulted in hyperacetylation levels of histone H3 at lysine 9 (AcH3K9) and histone H4 at lysine 5 (AcH4K5) at pronuclear, two-cell, and four-cell stages partly through downregulating HDAC1. The suppression of HDAC6 through oxamflatin increased the nonhistone acetylation level of α-tubulin during the mitotic cell cycle of early SCNT embryos. In addition, we demonstrated that oxamflatin downregulated DNA methyltransferase 1 (DNMT1) expression and global DNA methylation level (5-methylcytosine) in two-cell-stage porcine SCNT embryos. The pluripotency-related gene POU5F1 was found to be upregulated in the oxamflatin-treated group with a decreased DNA methylation tendency in its promoter regions. Treatment of oxamflatin did not change the locus-specific DNA methylation levels of Sus scrofa heterochromatic satellite DNA sequences at the blastocyst stage. Meanwhile, our findings suggest that treatment with HDACi may contribute to maintaining the stable status of cytoskeleton-associated elements, such as acetylated α-tubulin, which may be the crucial determinants of donor nuclear reprogramming in early SCNT embryos. In summary, oxamflatin treatment improves the developmental potential of porcine SCNT embryos in vitro. PMID

  6. Antiviral effects of bovine interferons on bovine respiratory tract viruses.

    OpenAIRE

    Fulton, R W; Downing, M M; Cummins, J M

    1984-01-01

    The antiviral effects of bovine interferons on the replication of bovine respiratory tract viruses were studied. Bovine turbinate monolayer cultures were treated with bovine interferons and challenged with several bovine herpesvirus 1 strains, bovine viral diarrhea virus, parainfluenza type 3 virus, goat respiratory syncytial virus, bovine respiratory syncytial virus, bovine adenovirus type 7, or vesicular stomatitis virus. Treatment with bovine interferons reduced viral yield for each of the...

  7. Nuclear overhauser enhancement mediated chemical exchange saturation transfer imaging at 7 Tesla in glioblastoma patients.

    Directory of Open Access Journals (Sweden)

    Daniel Paech

    Full Text Available BACKGROUND AND PURPOSE: Nuclear Overhauser Enhancement (NOE mediated chemical exchange saturation transfer (CEST is a novel magnetic resonance imaging (MRI technique on the basis of saturation transfer between exchanging protons of tissue proteins and bulk water. The purpose of this study was to evaluate and compare the information provided by three dimensional NOE mediated CEST at 7 Tesla (7T and standard MRI in glioblastoma patients. PATIENTS AND METHODS: Twelve patients with newly diagnosed histologically proven glioblastoma were enrolled in this prospective ethics committee-approved study. NOE mediated CEST contrast was acquired with a modified three-dimensional gradient-echo sequence and asymmetry analysis was conducted at 3.3 ppm (B1 = 0.7 µT to calculate the magnetization transfer ratio asymmetry (MTR(asym. Contrast enhanced T1 (CE-T1 and T2-weighted images were acquired at 3T and used for data co-registration and comparison. RESULTS: Mean NOE mediated CEST signal based on MTR(asym values over all patients was significantly increased (p<0.001 in CE-T1 tumor (-1.99 ± 1.22%, tumor necrosis (-1.36 ± 1.30% and peritumoral CEST hyperintensities (PTCH within T2 edema margins (-3.56 ± 1.24% compared to contralateral normal appearing white matter (-8.38 ± 1.19%. In CE-T1 tumor (p = 0.015 and tumor necrosis (p<0.001 mean MTR(asym values were significantly higher than in PTCH. Extent of the surrounding tumor hyperintensity was smaller in eight out of 12 patients on CEST than on T2-weighted images, while four displayed at equal size. In all patients, isolated high intensity regions (0.40 ± 2.21% displayed on CEST within the CE-T1 tumor that were not discernible on CE-T1 or T2-weighted images. CONCLUSION: NOE mediated CEST Imaging at 7 T provides additional information on the structure of peritumoral hyperintensities in glioblastoma and displays isolated high intensity regions within the CE-T1 tumor that cannot be acquired on CE-T1 or T2

  8. Lymphoid lineage differentiation potential of mouse nuclear transfer embryonic stem cells.

    Science.gov (United States)

    Eslami-Arshaghi, Tarlan; Salehi, Mohammad; Soleimani, Masoud; Gholipourmalekabadi, Mazaher; Mossahebi-Mohammadi, Majid; Ardeshirylajimi, Abdolreza; Rajabi, Hoda

    2015-09-01

    Stem cells therapy is considered as an efficient strategy for the treatment of some diseases. Nevertheless, some obstacles such as probability of rejection by the immune system limit applications of this strategy. Therefore, several efforts have been made to overcome this among which using the induced pluripotent stem cells (iPSCs) and nuclear transfer embryonic stem cell (nt-ESCs) are the most efficient strategies. The objective of this study was to evaluate the differentiation potential of the nt-ESCs to lymphoid lineage in the presence of IL-7, IL-3, FLT3-ligand and TPO growth factors in vitro. To this end, the nt-ESCs cells were prepared and treated with aforementioned growth factors for 7 and 14 days. Then, the cells were examined for expression of lymphoid markers (CD3, CD25, CD127 and CD19) by quantitative PCR (q-PCR) and flow cytometry. An increased expression of CD19 and CD25 markers was observed in the treated cells compared with the negative control samples by day 7. After 14 days, the expression level of all the tested CD markers significantly increased in the treated groups in comparison with the control. The current study reveals the potential of the nt-ESCs in differentiation to lymphoid lineage in the presence of defined growth factors.

  9. Nuclear retinoid receptors and pregnancy: placental transfer, functions, and pharmacological aspects.

    Science.gov (United States)

    Comptour, Aurélie; Rouzaire, Marion; Belville, Corinne; Bouvier, Damien; Gallot, Denis; Blanchon, Loïc; Sapin, Vincent

    2016-10-01

    Animal models of vitamin A (retinol) deficiency have highlighted its crucial role in reproduction and placentation, whereas an excess of retinoids (structurally or functionally related entities) can cause toxic and teratogenic effects in the embryo and foetus, especially in the first trimester of human pregnancy. Knock-out experimental strategies-targeting retinoid nuclear receptors RARs and RXRs have confirmed that the effects of vitamin A are mediated by retinoic acid (especially all-trans retinoic acid) and that this vitamin is essential for the developmental process. All these data show that the vitamin A pathway and metabolism are as important for the well-being of the foetus, as they are for that of the adult. Accordingly, during this last decade, extensive research on retinoid metabolism has yielded detailed knowledge on all the actors in this pathway, spurring the development of antagonists and agonists for therapeutic and research applications. Natural and synthetic retinoids are currently used in clinical practice, most often on the skin for the treatment of acne, and as anti-oncogenic agents in acute promyelocytic leukaemia. However, because of the toxicity and teratogenicity of retinoids during pregnancy, their pharmacological use needs a sound knowledge of their metabolism, molecular aspects, placental transfer, and action. PMID:27502420

  10. Optical pumping and population transfer of nuclear-spin states of caesium atoms in high magnetic fields

    Institute of Scientific and Technical Information of China (English)

    Luo Jun; Sun Xian-Ping; Zeng Xi-Zhi; Zhan Ming-Sheng

    2007-01-01

    Nuclear-spin states of gaseous-state Cs atoms in the ground state are optically manipulated using a Ti:sapphire laser in a magnetic field of 1.516 T, in which optical coupling of the nuclear-spin states is achieved through hyperfine interactions between electrons and nuclei. The steady-state population distribution in the hyperfine Zeeman sublevels of the ground state is detected by using a tunable diode laser. Furthermore, the state population transfer among the of Cs in the ground state due to stochastic collisions between Cs atoms and buffer-gas molecules, is studied at different of the hyperfine interaction can strongly cause the state population transfer and spin-state interchange among the hyperfine Zeeman sublevels. The calculated results maybe explain the steady-state population in hyperfine Zeeman sublevels in terms of rates of optical-pumping, electron-spin flip, nuclear spin flip, and electron-nuclear spin flip-flop transitions among the hyperfine Zeeman sublevels of the ground state of Cs atoms. This method may be applied to the nuclear-spin-based solid-state quantum computation.

  11. In vitro development of OPU-derived bovine embryos cultured either individually or in groups with the silk protein sericin and the viability of frozen-thawed embryos after transfer.

    Science.gov (United States)

    Isobe, Tomohiro; Ikebata, Yoshihisa; Do, Lanh Thi Kim; Tanihara, Fuminori; Taniguchi, Masayasu; Otoi, Takeshige

    2015-07-01

    The optimization of single-embryo culture conditions is very important, particularly in the in vitro production of bovine embryos using the ovum pick-up (OPU) procedure. The purpose of this study was to examine the development of embryos derived from oocytes obtained by OPU that were cultured either individually or in groups in medium supplemented with or without sericin and to investigate the viability of the frozen-thawed embryos after a direct transfer. When two-cell-stage embryos were cultured either individually or in groups for 7 days in CR1aa medium supplemented with or without 0.5% sericin, the rates of development to blastocysts and freezable blastocysts were significantly lower for the embryos cultured individually without sericin than for the embryos cultured in groups with or without sericin. Moreover, the rate of development to freezable blastocysts of the embryos cultured individually with sericin was significantly higher than that of the embryos cultured without sericin. When the frozen-thawed embryos were transferred directly to recipients, the rates of pregnancy, abortion, stillbirth and normal calving in the recipients were similar among the groups, irrespective of the culture conditions and sericin supplementation. Our findings indicate that supplementation with sericin during embryo culture improves the quality of the embryos cultured individually but not the viability of the frozen-thawed embryos after transfer to recipients. PMID:25488699

  12. The role of tacit knowledge and the challenges in transferring it in the nuclear power plant context

    International Nuclear Information System (INIS)

    Explicit knowledge and implementation of proper IT systems have been of concern of many organizations which recognize the importance of managing knowledge for safety operation and sustainable competitive advantage. Data and document management systems have been implemented to capture, store and distribute explicit knowledge. However, recently also awareness of the existence of tacit knowledge in organizations has arisen. What kind of role this undocumented knowledge, which the employees have acquired through their careers, plays in nuclear power plant context? This paper explores the role of tacit knowledge and the challenges related to its transfer at the two Finnish nuclear power plants. (author)

  13. Desarrollo de embriones de bovino obtenidos por fecundación in vitro cultivados con células oviductales o medio condicionado y transferidos a hembras receptoras Bovine embryo development produced by in vitro fertilization cultured with oviductal cell or conditioned medium and transfer to recipients

    Directory of Open Access Journals (Sweden)

    M RATTO

    1999-01-01

    Full Text Available Se comparó el desarrollo in vitro de ovocitos obtenidos de ovarios de vaca de matadero, madurados, fecundados y cultivados in vitro bajo dos sistemas. Los ovocitos fueron cultivados en un medio de maduración a 39 °C, 5 % de CO2 y humedad relativa de 95 % durante 22 horas. Posteriormente, fueron incubados con espermatozoides seleccionados a través de una gradiente discontinua de Percoll. La tasa de maduración nuclear y fecundación fueron de 93,7 % (74/79 y 76,9 % (50/65 respectivamente. Un total de 252 ovocitos fecundados fueron cultivados in vitro. El porcentaje de desarrollo in vitro a las 2 días post-inseminación (embriones de 4-8 células fue de 62,7 % (64/102 para los cigotos cultivados con células oviductales y de 67 % (100/150 para los cultivados en medio condicionado (P0,05. El porcentaje de desarrollo de mórulas fue de 17,6 % (18/102 para los cigotos cultivados con células oviductales y de 13,3 % (20/150 para los cultivados con medio condicionado (P0,05. Se obtuvo una tasa de desarrollo del 15,7 % (16/102 de blastocistos para aquellos cigotos cultivados con células oviductales. No se obtuvo blastocistos a partir de cigotos cultivados en medio condicionado. Cuatro blastocistos fueron transferidos a dos hembras receptoras. A los 42 y 57 días se encontró la presencia de un feto en cada hembraThe in vitro development of matured and fertilized bovine oocytes was compared between two culture systems. Oocytes were collected by aspiration of follicles of 3-8 mm in diameter using an 18g needle. After morphological selection the oocytes were incubated at 39 0C, 5 % C02 y 95 % relative humidity, during 22 hours. Afterwards, oocytes were incubated with spermatozoa selected by Percoll gradient system. The rate of nuclear maturation and fertilization was 93,7 % (74/79 and 76,9 % (50/65, respectively. A total of 252 zygotes were cultured, 102 with oviductal cells and 150 in conditioned medium. The in vitro development on day 2 of culture

  14. Factors Affecting the Efficiency of Embryonic Cell Nuclear Transfer in Rabbits

    Institute of Scientific and Technical Information of China (English)

    CUI Kui-qing; LIU Qing-you; XIE Ying; WEI Jing-wei; SHI De-shun

    2005-01-01

    Factors affecting the efficiency of nuclear transfer (NT) in rabbits were examined in the present study. When 100 V mm-1of pulse strength and 15 μs of pulse duration were employed, 3 and 4 electronic pulses resulted in significantly more cytoplasts fused with donor cells compared with 2 electronic pulses (P<0.05), but no significant difference was found in the cleavage rate of reconstructed embryos among the three groups (P> 0.05). When the duration and number of electronic pulse were fixed at 15 μs and 3 times, increase of pulse intensity from 100 V mm-1 to 150 V mm-1 and 200 V mm-1 resulted in a significantly decrease in the cleavage rate of reconstructed embryos (P < 0.05), although the fusion rate did not significantly differ among the three groups (P > 0.05). Significantly more reconstructed embryos cleaved and developed to blastocysts when they were derived from donor embryos at the 8-16-cell stage, in comparison with the reconstructed embryos derived from donor embryos at the compact morula stage (P < 0.05), although the fusion rate was similar (P > 0.05). Activation of cytoplasts prior to fusion increased the cleavage rate (P < 0.05) and blastocyst development (P < 0.05) of reconstructed embryos, but decreased the fusion rate (P < 0.05) compared with cytoplasts activated post fusion. More reconstructed embryos developed to blastocysts when they were cultured in TCM + 3% OCS at the first 48 h and then cultured in TCM199+ 10% FCS, in comparison with the reconstructed embryos cultured in either TCM199+ 10% FCS or TCM199+ 3% OCS (P < 0.05). When 22 NT embryos were transferred into the oviducts of one recipient rabbit, one recipient rabbit delivered a female rabbit at 34 days of gestation. In conclusion, either electrofusion parameter or developmental stage of donor embryos have a significant effect on the efficiency of NT, NT embryos require different concentration of serum at their different development stages.

  15. Trichostatin A specifically improves the aberrant expression of transcription factor genes in embryos produced by somatic cell nuclear transfer

    OpenAIRE

    Kimiko Inoue; Mami Oikawa; Satoshi Kamimura; Narumi Ogonuki; Toshinobu Nakamura; Toru Nakano; Kuniya Abe; Atsuo Ogura

    2015-01-01

    Although mammalian cloning by somatic cell nuclear transfer (SCNT) has been established in various species, the low developmental efficiency has hampered its practical applications. Treatment of SCNT-derived embryos with histone deacetylase (HDAC) inhibitors can improve their development, but the underlying mechanism is still unclear. To address this question, we analysed gene expression profiles of SCNT-derived 2-cell mouse embryos treated with trichostatin A (TSA), a potent HDAC inhibitor t...

  16. Transfer of suspended particles from liquid effluents of nuclear generating stations through the environment

    International Nuclear Information System (INIS)

    Due to the complexity of the environmental transfer of suspended particles in aquatic systems, the available literature usually deals with specific pathways and mechanisms of the transfer process. This paper attempts to give a brief overview of the entire transfer process. Potential routes of transfer in both the marine and freshwater environments are examined, and tentative conclusions presented. This work was performed while the author was employed by Atomic Energy Control Board under the McMaster University cooperative program

  17. Vitamin C enhances in vitro and in vivo development of porcine somatic cell nuclear transfer embryos

    International Nuclear Information System (INIS)

    Highlights: → Report for the first time that vitamin C has a beneficial effect on the development of porcine SCNT embryos. → The level of acH4K5 and Oct4 expression at blastocyst-stage was up-regulated after treatment. → A higher rate of gestation and increased number of piglets born were harvested in the treated group. -- Abstract: The reprogramming of differentiated cells into a totipotent embryonic state through somatic cell nuclear transfer (SCNT) is still an inefficient process. Previous studies revealed that the generation of induced pluripotent stem (iPS) cells from mouse and human fibroblasts could be significantly enhanced with vitamin C treatment. Here, we investigated the effects of vitamin C, to our knowledge for the first time, on the in vitro and in vivo development of porcine SCNT embryos. The rate of blastocyst development in SCNT embryos treated with 50 μg/mL vitamin C 15 h after activation (36.0%) was significantly higher than that of untreated SCNT embryos (11.5%). The enhanced in vitro development rate of vitamin C-treated embryos was associated with an increased acetylation level of histone H4 lysine 5 and higher Oct4, Sox2 and Klf4 expression levels in blastocysts, as determined by real-time PCR. In addition, treatment with vitamin C resulted in an increased pregnancy rate in pigs. These findings suggest that treatment with vitamin C is beneficial for enhancement of the in vitro and in vivo development of porcine SCNT embryos.

  18. Vitamin C enhances in vitro and in vivo development of porcine somatic cell nuclear transfer embryos

    Energy Technology Data Exchange (ETDEWEB)

    Huang, Yongye; Tang, Xiaochun; Xie, Wanhua; Zhou, Yan; Li, Dong; Zhou, Yang; Zhu, Jianguo; Yuan, Ting; Lai, Liangxue [Jilin Province Key Laboratory of Animal Embryo Engineering, College of Animal Science and Veterinary Medicine, Jilin University, 5333 Xi An DaLu, Changchun 130062 (China); Pang, Daxin, E-mail: pdx@jlu.edu.cn [Jilin Province Key Laboratory of Animal Embryo Engineering, College of Animal Science and Veterinary Medicine, Jilin University, 5333 Xi An DaLu, Changchun 130062 (China); Ouyang, Hongsheng, E-mail: ouyh@jlu.edu.cn [Jilin Province Key Laboratory of Animal Embryo Engineering, College of Animal Science and Veterinary Medicine, Jilin University, 5333 Xi An DaLu, Changchun 130062 (China)

    2011-07-29

    Highlights: {yields} Report for the first time that vitamin C has a beneficial effect on the development of porcine SCNT embryos. {yields} The level of acH4K5 and Oct4 expression at blastocyst-stage was up-regulated after treatment. {yields} A higher rate of gestation and increased number of piglets born were harvested in the treated group. -- Abstract: The reprogramming of differentiated cells into a totipotent embryonic state through somatic cell nuclear transfer (SCNT) is still an inefficient process. Previous studies revealed that the generation of induced pluripotent stem (iPS) cells from mouse and human fibroblasts could be significantly enhanced with vitamin C treatment. Here, we investigated the effects of vitamin C, to our knowledge for the first time, on the in vitro and in vivo development of porcine SCNT embryos. The rate of blastocyst development in SCNT embryos treated with 50 {mu}g/mL vitamin C 15 h after activation (36.0%) was significantly higher than that of untreated SCNT embryos (11.5%). The enhanced in vitro development rate of vitamin C-treated embryos was associated with an increased acetylation level of histone H4 lysine 5 and higher Oct4, Sox2 and Klf4 expression levels in blastocysts, as determined by real-time PCR. In addition, treatment with vitamin C resulted in an increased pregnancy rate in pigs. These findings suggest that treatment with vitamin C is beneficial for enhancement of the in vitro and in vivo development of porcine SCNT embryos.

  19. Rex Rabbit Somatic Cell Nuclear Transfer with In Vitro-Matured Oocytes.

    Science.gov (United States)

    Liu, Yong; Wang, Huili; Lu, Jinhua; Miao, Yiliang; Cao, Xinyan; Zhang, Ling; Wu, Xiaoqing; Wu, Fengrui; Ding, Biao; Wang, Rong; Luo, Mingjiu; Li, Wenyong; Tan, Jinghe

    2016-06-01

    Somatic cell nuclear transfer (SCNT) requires large numbers of matured oocytes. In vitro-matured (IVM) oocytes have been used in SCNT in many animals. We investigated the use of IVM oocytes in Rex rabbit SCNT using Rex rabbit ovaries obtained from a local abattoir. The meiotic ability of oocytes isolated from follicles of different diameters was studied. Rex rabbit SCNT was optimized for denucleation, activation, and donor cell synchronization. Rex rabbit oocytes grew to the largest diameter (110 μm) when the follicle diameter was 1.0 mm. Oocytes isolated from 0.7-mm follicles acquired maturation ability. More than 90% of these oocytes matured after IVC for 18 h. The developmental potential of oocytes isolated from >1-mm follicles was greater than that of oocytes isolated from 0.7- to 1.0-mm follicles. The highest activation rates for IVM Rex rabbit oocytes were seen after treatment with 2.5 μM ionomycin for 5 min followed by 2 mM 6-dimethylaminopurine (6-DMAP) and 5 μg/mL cycloheximide (CHX) for 1 h. Ionomycin induced the chromatin of IVM oocytes to protrude from the oocyte surface, promoting denucleation. Fetal fibroblast cells (FFCs) and cumulus cells (CCs) were more suitable for Rex rabbit SCNT than skin fibroblast cells (SFCs) (blastocyst rate was 35.6 ± 2.2% and 38.0 ± 6.0% vs. 19.7 ± 3.1%). The best fusion condition was a 2DC interval for 1 sec, 1.6 kV/cm voltages, and 40 μsec duration in 0.28 M mannitol. In conclusion, the in vitro maturation of Rex rabbit oocytes and SCNT procedures were studied systematically and optimized in this study. PMID:27159389

  20. Frequent somatic transfer of mitochondrial DNA into the nuclear genome of human cancer cells

    NARCIS (Netherlands)

    Y.S. Ju (Young Seok); J.M.C. Tubio (Jose M.); W. Mifsud (William); B. Fu (Beiyuan); H. Davies (Helen); M. Ramakrishna (Manasa); Y. Li (Yilong); L.R. Yates (Lucy); G. Gundem (Gunes); P.S. Tarpey (Patrick); S. Behjati (Sam); E. Papaemmanuil (Elli); S. Martin; A. Fullam (Anthony); M. Gerstung (Moritz); J. Nangalia (Jyoti); A.R. Green (Anthony R.); C. Caldas (Carlos); Å. Borg (Åke); A. Tutt (Andrew); M.T. Michael Lee (Ming Ta); L.J. van 't Veer (Laura); B.K.T. Tan (Benita K.T.); S.A.J.R. Aparicio (Samuel A. J.); P.N. Span (Paul); J.W.M. Martens (John W. M.); S. Knappskog (Stian); A. Vincent-Salomon (Anne); A.-L. Borresen-Dale (Anne-Lise); J. Eyfjord; A.M. Flanagan (Adrienne); C.S. Foster; D. Neal (David); C. Cooper (Colin); R. Eeles (Rosalind); S. Lakhani (Sunil); C. Desmedt (Christine); G. Thomas (Gilles); A.L. Richardson (Andrea); C.A. Purdie (Colin A.); A.M. Thompson (Alastair M.); U. McDermott (Ultan); F. Yang (Fengtang); S. Nik-Zainal (Serena); P.J. Campbell (Peter); M.R. Stratton (Michael)

    2015-01-01

    textabstractMitochondrial genomes are separated from the nuclear genome for most of the cell cycle by the nuclear double membrane, intervening cytoplasm, and the mitochondrial double membrane. Despite these physical barriers, we show that somatically acquired mitochondrial-nuclear genome fusion sequ

  1. Efficient production of omega-3 fatty acid desaturase (sFat-1)-transgenic pigs by somatic cell nuclear transfer

    Institute of Scientific and Technical Information of China (English)

    2010-01-01

    Omega-3(ω-3) fatty acid desaturase transgenic pigs may improve carcass fatty acid composition. The use of transgenic pigs is also an excellent large animal model for studying the role of ω-3 fatty acids in the prevention and treatment of coronary heart disease and cancer. Transgenic pigs carrying synthesized fatty acid desaturase-1 gene (sFat-1) from Caenorhabditis briggsae by somatic cell nuclear transfer (SCNT) were produced for the first time in China. Porcine fetal fibroblast cells were transfected with a sFat-1 expression cassette by the liposome-mediated method. Transgenic embryos were reconstructed by nuclear transfer of positive cells into enucleated in vitro matured oocytes. A total of 1889 reconstructed embryos were transferred into 10 naturally cycling gilts. Nine early pregnancies were established, 7 of which went to term. Twenty-one piglets were born. The cloning efficiency was 1.1% (born piglets/transferred embryos). The integration of the sFat-1 gene was confirmed in 15 live cloned piglets by PCR and Southern blot except for 2 piglets. Expression of the sFat-1 gene in 12 of 13 piglets was detected with RT-PCR. The data demonstrates that an efficient system for sFat-1 transgenic cloned pigs was developed, which led to the successful production of piglets expressing the sFat-1 gene.

  2. Soil- to-Whole Body Transfer Factors of Radionuclides for Terrestrial Wild Animals Living around the Gyeongju Nuclear Site

    International Nuclear Information System (INIS)

    In the Gyeongju nuclear site, a low and medium level radioactive waste repository and five nuclear reactors are currently operating. The IAEA and ICRP consider it necessary to demonstrate that not only men but also wild organisms are protected from ionizing radiations. Therefore, it may not be long before the dose assessment for wildlife should be carried out in Korea. The transfer factor (TF) defined as the concentration ratio between an organism and an environmental medium is a key parameter in assessing the radiation dose to wildlife. For eight animal species inhabiting mountainous areas around the Gyeongju nuclear site, the TF values of 22 stable elements were measured. The acquired values varied considerably with the elements and animal species. Further TF data need to be produced for various Korean wild animal and plant species in preparation for future governmental regulations of wildlife exposure to ionizing radiations

  3. Nuclear dynamics investigation of the initial electron transfer in the cyclobutane pyrimidine dimer lesion repair process by photolyases

    CERN Document Server

    Joubert-Doriol, Loic; Olivucci, Massimo; Izmaylov, Artur F

    2016-01-01

    Photolyases are proteins capable of harvesting the sunlight to repair DNA damages caused by UV light. In this work we focus on the first step in the repair process of the cyclobutane pyrimidine dimer photoproduct (CPD) lesion, which is an electron transfer (ET) from a flavine cofactor to CPD, and study the role of various nuclear degrees of freedom (DOF) in this step. The ET step has been experimentally studied using transient spectroscopy and the corresponding data provide excellent basis for testing the quality of quantum dynamical models. Based on previous theoretical studies of electronic structure and conformations of the protein active site, we present a procedure to build a diabatic Hamiltonian for simulating the ET reaction in a molecular complex mimicking the enzyme's active site. We generate a reduced nuclear dimensional model that provides a first non-empirical quantum dynamical description of the structural features influencing the ET rate. By varying the nuclear DOF parametrization in the model t...

  4. Soil- to-Whole Body Transfer Factors of Radionuclides for Terrestrial Wild Animals Living around the Gyeongju Nuclear Site

    Energy Technology Data Exchange (ETDEWEB)

    Lim, Kwang-Muk; Choi, Yong-Ho; Jun, In; Kim, Byung-Ho; Keum, Dong-Kwon [Korea Atomic Energy Research Institute, Daejeon (Korea, Republic of)

    2015-05-15

    In the Gyeongju nuclear site, a low and medium level radioactive waste repository and five nuclear reactors are currently operating. The IAEA and ICRP consider it necessary to demonstrate that not only men but also wild organisms are protected from ionizing radiations. Therefore, it may not be long before the dose assessment for wildlife should be carried out in Korea. The transfer factor (TF) defined as the concentration ratio between an organism and an environmental medium is a key parameter in assessing the radiation dose to wildlife. For eight animal species inhabiting mountainous areas around the Gyeongju nuclear site, the TF values of 22 stable elements were measured. The acquired values varied considerably with the elements and animal species. Further TF data need to be produced for various Korean wild animal and plant species in preparation for future governmental regulations of wildlife exposure to ionizing radiations.

  5. Communications received from certain Member States regarding guidelines for the export of nuclear material, equipment and technology. Nuclear-related dual-use transfers

    International Nuclear Information System (INIS)

    The Director General has received notes verbales dated 30 June 1995 from the Resident Representatives to the Agency of Argentina, Australia, Austria, Belgium, Bulgaria, Canada, the Czech Republic, Denmark, Finland, France, Germany, Greece, Hungary, Ireland, Italy, Japan, Luxembourg, the Netherlands, New Zealand, Norway, Poland, Portugal, Romania, the Slovak Republic, South Africa, Spain, Sweden, Switzerland, the United Kingdom of Great Britain and Northern Ireland, and the United States of America relating to the export of nuclear material, equipment and technology. The purpose of the notes verbales is to provide further information on those Governments' Guidelines for Transfers of Nuclear-related Dual-use Equipment, Material and related Technology. In the light of the wish expressed at the end of each note verbale, the text of the notes verbales is annexed hereto. The enclosure to these notes verbales is also reproduced in full in the Annex

  6. Theoretical analysis and numerical modeling of heat transfer and fuel migration in underlying soils and constructive elements of nuclear plants during an accident release from the core

    International Nuclear Information System (INIS)

    Migration of fuel fragments and core fission products during severe accidents on nuclear plants is studied analytically and numerically.The problems of heat transfer and migration of volume heat sources in construction materials and underlying soils are considered

  7. The pro-inflammatory cytokine tumor necrosis factor α stimulates expression of the carnitine transporter OCTN2 (novel organic cation transporter 2) and carnitine uptake via nuclear factor-κB in Madin-Darby bovine kidney cells.

    Science.gov (United States)

    Zhou, X; Ringseis, R; Wen, G; Eder, K

    2015-06-01

    Carnitine uptake into tissues is mediated mainly by the novel organic cation transporter 2 (OCTN2), whose expression is upregulated in the liver of early-lactating dairy cows. It has been shown recently that pro-inflammatory cytokines, including tumor necrosis factor α (TNFα), stimulate OCTN2 expression and carnitine uptake in intestinal cells and inflamed intestinal mucosa. Given that many early-lactating dairy cows show typical signs of hepatic and systemic inflammation, such as elevated concentrations of circulating TNFα and activation of the key regulator of inflammation, nuclear factor κB (NF-κB), in tissues, it is possible that upregulation of OCTN2 and increase of carnitine uptake by TNFα is mediated by NF-κB, a mechanism that might contribute to the upregulation of OCNT2 in the liver of early-lactating dairy cows. Thus, in the present study, we tested the hypothesis that TNFα stimulates OCTN2 gene expression and carnitine uptake via NF-κB in the bovine Madin-Darby bovine kidney (MDBK) cell line. Treatment with TNFα caused activation of NF-κB, increased the mRNA and protein concentration of OCTN2, and stimulated the uptake of carnitine in MDBK cells. In contrast, combined treatment of MDBK cells with TNFα and the NF-κB inhibitor BAY 11-7085 completely blocked the effect of TNFα on OCTN2 mRNA and protein concentration and uptake of carnitine. These findings suggest that the bovine OCTN2 gene and carnitine uptake are regulated by NF-κB. Future studies are required to show the in vivo relevance of this regulatory mechanism in cattle.

  8. Global protest against nuclear power: transfer and transnational exchange in the 1970s and 1980s

    OpenAIRE

    Kirchhof, Astrid Mignon; Meyer, Jan-Henrik

    2014-01-01

    "Protest against nuclear power plants, uranium mining and nuclear testing played a pivotal role in the rise of a mass environmental movement around the globe in the 1970s and 1980s. Nevertheless, the history of anti-nuclear activism has largely been told from a strictly national perspective. This HSR focus approaches the phenomenon from a transnational perspective for the first time. Against the backdrop of the debate on transnational history, this article develops a framework of analysis, an...

  9. Nuclear transfer of synchronized African wild cat somatic cells into enucleated domestic cat oocytes

    Science.gov (United States)

    Gomez, M.C.; Jenkins, J.A.; Giraldo, A.; Harris, R.F.; King, A.; Dresser, B.L.; Pope, C.E.

    2003-01-01

    The African wild cat is one of the smallest wild cats and its future is threatened by hybridization with domestic cats. Nuclear transfer, a valuable tool for retaining genetic variability, offers the possibility of species continuation rather than extinction. The aim of this study was to investigate the ability of somatic cell nuclei of the African wild cat (AWC) to dedifferentiate within domestic cat (DSH) cytoplasts and to support early development after nuclear transplantation. In experiment 1, distributions of AWC and DSH fibroblasts in each cell-cycle phase were assessed by flow cytometry using cells cultured to confluency and disaggregated with pronase, trypsin, or mechanical separation. Trypsin (89.0%) and pronase (93.0%) yielded higher proportions of AWC nuclei in the G0/G1 phase than mechanical separation (82.0%). In contrast, mechanical separation yielded higher percentages of DSH nuclei in the G0/G1 phase (86.6%) than pronase (79.7%) or trypsin (74.2%) treatments. In both species, pronase induced less DNA damage than trypsin. In experiment 2, the effects of serum starvation, culture to confluency, and exposure to roscovitine on the distribution of AWC and DSH fibroblasts in various phases of the cell cycle were determined. Flow cytometry analyses revealed that the dynamics of the cell cycle varied as culture conditions were modified. Specifically, a higher percentage of AWC and DSH nuclei were in the G0/G1 phase after cells were serum starved (83% vs. 96%) than were present in cycling cells (50% vs. 64%), after contact inhibition (61% vs. 88%), or after roscovitine (56% vs. 84%) treatment, respectively. In experiment 3, we evaluated the effects of cell synchronization and oocyte maturation (in vivo vs. in vitro) on the reconstruction and development of AWC-DSH- and DSH-DSH-cloned embryos. The method of cell synchronization did not affect the fusion and cleavage rate because only a slightly higher percentage of fused couplets cleaved when donor nuclei

  10. Safeguards for spent nuclear fuel in transfer from wet storage to dry storage in on-site interim storage facilities

    International Nuclear Information System (INIS)

    Germany initially planned to store spent nuclear fuel in the two away-from-reactor interim storage facilities built at Ahaus and Gorleben. The current approach for spent fuel management is on-site interim storage in transport and storage casks as part of a political agreement between the German government and the nuclear operators on the future use of nuclear energy. A reason for this is to avoid near term transportation of spent fuel determined for direct final disposal via public road or rail systems to away-from-reactor storage facilities. Recent legislation has triggered the construction of 12 on-site dry storage facilities at nuclear power plants. Currently, such facilities are being taken into operation on a step-by-step basis. There is a strong need to develop acceptable safeguards concepts for both transfer and dry storage of spent fuel, ideally, a standard safeguards concept that would match all German nuclear power plant sites without ignoring technical and organisational differences. The paper will address the relevant issues and give an overview of the status of safeguards implementation. (author)

  11. Association between mitochondrial DNA haplotype compatibility and increased efficiency of bovine intersubspecies cloning

    Institute of Scientific and Technical Information of China (English)

    Hao Yan; Zhonghai Yan; Qingwen Ma; Fei Jiao; Shuzhen Huang; Fanyi Zeng; Yitao Zeng

    2011-01-01

    Reconstructed embryos derived from intersubspecies somatic cell nuclear transfer (SCNT) have poorer developmental potential than those from intrasubspecies SCNT. Based on our previous study that Holstein dairy bovine (HD) mitochondrial DNA (mtDNA) haplotype compatibility between donor karyoplast and recipient cytoplast is crucial for SCNT embryo development, we performed intersubspecies SCNT using HD as donor karyoplast and Luxi yellow heifer (LY) as recipient cytoplast according to mtDNA haplotypes determined by polymerase chain reactionrestriction fragment length polymorphism (PCR-RFLP) analysis. The results demonstrated that intersubspecies mtDNA homotype SCNT embryos had higher pre- and post-implantation developmental competence than intrasubspecies mtDNA heterotype embryos as well as improved blastocyst reprogramming status, including normal H3K9 dimethylation pattern and promoter hypomethylation of pluripotent genes such as Oct4 and Sox2, suggesting that intersubspecies SCNT using LY oocytes maintains HD cloning efficiency and may reprogram HD nuclei to develop into a normal cloned animal ultimately. Our results indicated that karyoplast-cytoplast interactions and mtDNA haplotype compatibility may affect bovine intersubspecies SCNT efficiency. This study on bovine intersubspecies SCNT is valuable for understanding the mechanisms of mtDNA haplotype compatibility between karyoplast and cytoplast impacting the bovine SCNT efficiency, and provides an alternative and economic resource for HD cloning.

  12. Oocyte-secreted factors in oocyte maturation media enhance subsequent development of bovine cloned embryos.

    Science.gov (United States)

    Su, Jianmin; Wang, Yongsheng; Zhang, Lei; Wang, Bo; Liu, Jun; Luo, Yan; Guo, Zekun; Quan, Fusheng; Zhang, Yong

    2014-04-01

    Successful in vitro maturation (IVM) and oocyte quality both affect the subsequent development of cloned embryos derived from somatic-cell nuclear transfer (SCNT). Developmental competence is usually lower in oocytes matured in vitro compared with those that matured in vivo, possibly due to insufficient levels of oocyte-secreted factors (OSFs) and disrupted oocyte-cumulus communication. This study investigated the effects of OSFs secreted by denuded oocytes (DOs) during IVM on the subsequent developmental competence of cloned bovine embryos. Cumulus-oocyte complexes (COCs) from antral follicles of slaughtered-cow ovaries collected from an abattoir were divided into four groups: COCs co-cultured with and without DOs in maturation media used for SCNT, as well as COCs co-cultured with and without DOs in maturation media used for in vitro fertilization (IVF). Based on the developmental competence and embryo quality of bovine embryos generated from these four groups, we found that co-culturing the COCs with DOs enhanced the in vitro development of IVF and cloned bovine embryos, and potentially generated more high-quality cloned blastocysts that possessed locus-specific histone modifications at levels similar to in vitro-fertilized embryos. These results strongly suggest that co-culturing COCs with DOs enhances subsequent developmental competence of cloned bovine embryo. PMID:24420374

  13. Use of the 2A peptide for generation of multi-transgenic pigs through a single round of nuclear transfer.

    Directory of Open Access Journals (Sweden)

    Wei Deng

    Full Text Available Multiple genetic modifications in pigs can essentially benefit research on agriculture, human disease and xenotransplantation. Most multi-transgenic pigs have been produced by complex and time-consuming breeding programs using multiple single-transgenic pigs. This study explored the feasibility of producing multi-transgenic pigs using the viral 2A peptide in the light of previous research indicating that it can be utilized for multi-gene transfer in gene therapy and somatic cell reprogramming. A 2A peptide-based double-promoter expression vector that mediated the expression of four fluorescent proteins was constructed and transfected into primary porcine fetal fibroblasts. Cell colonies (54.3% formed under G418 selection co-expressed the four fluorescent proteins at uniformly high levels. The reconstructed embryos, which were obtained by somatic cell nuclear transfer and confirmed to express the four fluorescent proteins evenly, were transplanted into seven recipient gilts. Eleven piglets were delivered by two gilts, and seven of them co-expressed the four fluorescent proteins at equivalently high levels in various tissues. The fluorescence intensities were directly observed at the nose, hoof and tongue using goggles. The results suggest that the strategy of combining the 2A peptide and double promoters efficiently mediates the co-expression of the four fluorescent proteins in pigs and is hence a promising methodology to generate multi-transgenic pigs by a single nuclear transfer.

  14. Milk fatty acid composition and mammary lipogenic genes expression in bovine cloned and control cattle

    OpenAIRE

    Bernard, Laurence; Richard, Christophe; Gelin, Valerie; Leroux, Christine; Heyman, Yvan

    2015-01-01

    In order to understand the effect of nuclear transfer technology on the physiology of lactation and milk fatty acid composition in dairy cattle (Holstein breed), the present study compares the milk yield and composition from bovine somatic cell cloned (n=5) and control animals (n=5) at 180 days in milk (DIM) maintained together under the same conditions. All cows were offered the same total mixed ration ad libitum. At 180 DIM, the cloned had a higher body weight (BW; P<0.01) compared with ...

  15. Sensors and nuclear power. Report by the Technology Transfer Sensors Task Team

    Energy Technology Data Exchange (ETDEWEB)

    1985-06-01

    The existing sensor systems for the basic process parameters in nuclear power plant operation have limitations with respect to accuracy, ease of maintenance and signal processing. These limitations comprise the economy of nuclear power generation. To reduce the costs and improve performance of nuclear power plant fabrication, operation, maintenance and repair we need to advance the sensor technology being applied in the nuclear industry. The economic viability and public acceptance of nuclear power will depend on how well we direct and apply technological advances to the industry. This report was prepared by a team with members representing a wide range of the nuclear industry embracing the university programs, national laboratories, architect engineers and reactor manufacturers. An intensive effort was made to survey current sensor technology, evaluate future trends and determine development needs. This included literature surveys, visits with utilities, universities, laboratories and organizations outside the nuclear industry. Several conferences were attended to take advantage of the access to experts in selected topics and to obtain opinions. Numerous telephone contacts and exchanges by mail supplemented the above efforts. Finally, the broad technical depth of the team members provided the basis for the stimulating working sessions during which this report was organized and drafted.

  16. Sensors and nuclear power. Report by the Technology Transfer Sensors Task Team

    International Nuclear Information System (INIS)

    The existing sensor systems for the basic process parameters in nuclear power plant operation have limitations with respect to accuracy, ease of maintenance and signal processing. These limitations comprise the economy of nuclear power generation. To reduce the costs and improve performance of nuclear power plant fabrication, operation, maintenance and repair we need to advance the sensor technology being applied in the nuclear industry. The economic viability and public acceptance of nuclear power will depend on how well we direct and apply technological advances to the industry. This report was prepared by a team with members representing a wide range of the nuclear industry embracing the university programs, national laboratories, architect engineers and reactor manufacturers. An intensive effort was made to survey current sensor technology, evaluate future trends and determine development needs. This included literature surveys, visits with utilities, universities, laboratories and organizations outside the nuclear industry. Several conferences were attended to take advantage of the access to experts in selected topics and to obtain opinions. Numerous telephone contacts and exchanges by mail supplemented the above efforts. Finally, the broad technical depth of the team members provided the basis for the stimulating working sessions during which this report was organized and drafted

  17. Nuclear magnetic resonance study of charge transfer complex formation between Silver Nitrate and Benzylcyanide in Solvent Ethylene Glycol

    CERN Document Server

    Modarress, H

    2003-01-01

    The formation constant for charge transfer complexes between electron acceptor (AgNo sub 3) and electron donor benzylcyanide (C sub 6 H sub 5 -CH sub 2 -C ident to N) in solvent ethyleneglycol [(CH sub 2 OH) sub 2] has been evaluated by using the nuclear magnetic resonance chemical shifts of aromatic group of benzylcyanide measured against external references, tetramethylsilane, hexamethyldisilane and cyclohexane at 20 sup d ig sup C. The external referencing procedure eliminated the interference of internal reference in the course of complexation. The necessary bulk magnetic susceptibility corrections on the measured chemical shifts have been made. The solution nationalised and their effects on the formation constant have been considered and a new equation has been suggested to obtain the main ionic activity coefficient of AgNO sub 3 from nuclear magnetic resonance results. The mean ionic activity coefficient has been taken into account in the formation constant calculations. The results indicated that the a...

  18. Report of the Nuclear Energy Agency expert group on gut transfer factors: implications for dose per unit intake

    International Nuclear Information System (INIS)

    This note describes the gut transfer factors recommended by an Expert Group of the Nuclear Energy Agency for intakes of certain important elements in food and drinking water. The evidence behind the recommendations is discussed and their implications for dose per unit intake is investigated. It is found that in many cases the dose per unit intake calculated using the gut uptake factor recommended by the Expert Group is similar to that calculated using the recommendations of ICRP Publication 30. However, in some cases there are substantial increases in dose per unit intake. The largest increases are by a factor of fifty for intakes of certain thorium isotopes by infants. (author)

  19. An Epigenetic Modifier Results in Improved In Vitro Blastocyst production after Somatic Cell Nuclear Transfer

    DEFF Research Database (Denmark)

    Zhang, Yunhai; Li, Juan; Villemoes, Klaus;

    2007-01-01

    The present study was designed to examine the effect of trichostatin A (TSA), an inhibitor of histone deacetylase, on development of porcine cloned embryos. Our results showed that treatment of cloned embryos derived from sow oocytes with 50 nM TSA for up to 24 h after the onset of activation cou...... were tested, and for all cell lines an enhancement in blastocyst development compared to their corresponding control was observed. Our data demonstrate that TSA treatment after somatic cell nuclear transfer in the pig can significantly improve the in vitro blastocyst production...

  20. Development capacity of pre- and postpubertal pig oocytes evaluated by somatic cell nuclear transfer and parthenogenetic activation

    DEFF Research Database (Denmark)

    Skovsgaard, Hanne; Li, Rong; Liu, Ying;

    2013-01-01

    Most of the porcine oocytes used for in vitro studies are collected from gilts. Our aims were to study development capacity of gilt v. sow oocytes (pre- and postpubertal respectively) using 2 techniques illustrating development competence [parthenogenetic activation (PA) and somatic cell nuclear...... transfer (SCNT)], and to describe a simple method to select the most competent oocytes. Inside-ZP diameter of in vitro-matured gilt oocytes was measured (µm; small ≤110; medium >110; large ≥120). Gilt and sow oocytes were morphologically grouped as good (even cytoplasm, smooth cell membrane, visible...

  1. Workshop to exchange and transfer knowledge for the purpose of increasing public understanding relating to nuclear safety and to provide a forum for discussion of alternatives available to promote revitalization of nuclear power in the US

    International Nuclear Information System (INIS)

    This paper proposes an information dissemination program to adequately familiarize the public with the actual health and safety risks of nuclear energy development. It plans for a discussion panel for alternatives available to promote revitalization of nuclear power in the US. It also provides for technology transfer between contractors, designers, and training staff. It recognizes problem areas in licensing and certification by the Nuclear Regulatory Commission and ways to standardize the administrative procedures

  2. Conventional and Bimodal Nuclear Thermal Rocket (NTR) Artificial Gravity Mars Transfer Vehicle Concepts

    Science.gov (United States)

    Borowski, Stanley K.; McCurdy, David R.; Packard, Thomas W.

    2014-01-01

    A variety of countermeasures have been developed to address the debilitating physiological effects of "zero-gravity" (0-g) experienced by cosmonauts and astronauts during their approximately 0.5-1.2 year long stays in LEO (Low Earth Orbit). Longer interplanetary flights, combined with possible prolonged stays in Mars orbit, could subject crewmembers to up to approximately 2.5 years of weightlessness. In view of known and recently diagnosed problems associated with 0-g, an artificial gravity spacecraft offers many advantages and may indeed be an enabling technology for human flights to Mars. A number of important human factors must be taken into account in selecting the rotation radius, rotation rate, and orientation of the habitation module or modules. These factors include the gravity gradient effect, radial and tangential Coriolis forces, along with cross-coupled acceleration effects. Artificial gravity (AG) Mars transfer vehicle (MTV) concepts are presented that utilize both conventional NTR, as well as, enhanced "bimodal" nuclear thermal rocket (BNTR) propulsion. The NTR is a proven technology that generates high thrust and has a specific impulse (I (sub sp)) capability of approximately 900 s - twice that of today's best chemical rockets. The AG/MTV concepts using conventional NTP carry twin cylindrical "ISS-type" habitation modules with their long axes oriented either perpendicular or parallel to the longitudinal spin axis of the MTV and utilize photovoltaic arrays (PVAs) for spacecraft power. The twin habitat modules are connected to a central operations hub located at the front of the MTV via two pressurized tunnels that provide the rotation radius for the habitat modules. For the BNTR AG/MTV option, each engine has its own "closed" secondary helium-xenon gas loop and Brayton rotating unit that can generate tens of kilowatts (kW (sub e)) of spacecraft electrical power during the mission coast phase eliminating the need for large PVAs. A single inflatable

  3. The HIST1 Locus Escapes Reprogramming in Cloned Bovine Embryos

    Directory of Open Access Journals (Sweden)

    Byungkuk Min

    2016-05-01

    Full Text Available Epigenetic reprogramming is necessary in somatic cell nuclear transfer (SCNT embryos in order to erase the differentiation-associated epigenetic marks of donor cells. However, such epigenetic memories often persist throughout the course of clonal development, thus decreasing cloning efficiency. Here, we explored reprogramming-refractory regions in bovine SCNT blastocyst transcriptomes. We observed that histone genes residing in the 1.5 Mb spanning the cow HIST1 cluster were coordinately downregulated in SCNT blastocysts. In contrast, both the nonhistone genes of this cluster, and histone genes elsewhere remained unaffected. This indicated that the downregulation was specific to HIST1 histone genes. We found that, after trichostatin A treatment, HIST1 histone genes were derepressed, and DNA methylation at their promoters was decreased to the level of in vitro fertilization embryos. Therefore, our results indicate that the reduced expression of HIST1 histone genes is a consequence of poor epigenetic reprogramming in SCNT blastocysts.

  4. The Transfer and diffusion of Cesium 137 within forest ecosystem in Fukushima after the nuclear power plant accident

    International Nuclear Information System (INIS)

    A large amount of radionuclides was released from the Fukushima Daiichi Nuclear Power Plant (FDNPP) accident after the disastrous earthquake and subsequent tsunami of March 2011. Among the variety of radionuclides released from FDNPP, cesium 137 (137Cs) is the most worrying radionuclide in the environment, with a half-life of 30 years. Since most of the Japanese land area is covered by forest, the distribution and transportation of radioactive materials within forest ecosystems should be conscientiously monitored. In Europe, many studies reported that the 137Cs deposition caused by the Chernobyl accident has still been distributed in the litter and soil layers and has become a source for the soil-to-plant transfer. Most of these studies emphasize the 'stability' of 137Cs within forest ecosystems, because 137Cs are considered to be strongly and immediately fixed in clay minerals. Even though there are many studies of the soil-to-plant transfer of 137Cs in forest after several years of Chernobyl accident, very initial distribution and transfer of 137Cs in food web within one to two years after the deposition in forest ecosystems have never been examined. The evaluation of the initial dynamics of 137Cs in forest ecosystems should be quite important because of the increasing stability of 137Cs after the deposition. The accumulation and transfer of 137Cs through food web within forest ecosystems were examined by collecting various organisms at forests in Fukushima. The 137Cs concentrations, natural Cs and K concentrations, and delta 15N of the specimens were measured to evaluate the occurrence of bioaccumulation or bio-diffusion of 137Cs through tropic interaction within forest ecosystem. 137Cs was highly concentrated on leaf litters which had been deposited in autumn 2010, before the accident. This accumulated 137Cs had transferred to higher trophic organisms mainly through the detritus food chain, whereas the transfer through grazing food chain was relatively low at

  5. Generation of embryonic stem cells from mouse adipose-tissue derived cells via somatic cell nuclear transfer.

    Science.gov (United States)

    Qin, Yiren; Qin, Jilong; Zhou, Chikai; Li, Jinsong; Gao, Wei-Qiang

    2015-01-01

    Somatic cells can be reprogrammed into embryonic stem cells (ESCs) by nuclear transfer (NT-ESCs), or into induced pluripotent stem cells (iPSCs) by the "Yamanaka method." However, recent studies have indicated that mouse and human iPSCs are prone to epigenetic and transcriptional aberrations, and that NT-ESCs correspond more closely to ESCs derived from in vitro fertilized embryos than iPSCs. In addition, the procedure of NT-ESCs does not involve gene modification. Demonstration of generation of NT-ESCs using an easily-accessible source of adult cell types would be very important. Adipose tissue is a source of readily accessible donor cells and can be isolated from both males and females at different ages. Here we report that NT-ESCs can be generated from adipose tissue-derived cells (ADCs). At morphological, mRNA and protein levels, these NT-ESCs show classic ESC colonies, exhibit alkaline phosphatase (AP) activity, and display normal diploid karyotypes. Importantly, these cells express pluripotent markers including Oct4, Sox2, Nanog and SSEA-1. Furthermore, they can differentiate in vivo into various types of cells from 3 germinal layers by teratoma formation assays. This study demonstrates for the first time that ESCs can be generated from the adipose tissue by somatic cell nuclear transfer (SCNT) and suggests that ADCs can be a new donor-cell type for potential therapeutic cloning.

  6. Robotics and nuclear power. Report by the Technology Transfer Robotics Task Team

    International Nuclear Information System (INIS)

    A task team was formed at the request of the Department of Energy to evaluate and assess technology development needed for advanced robotics in the nuclear industry. The mission of these technologies is to provide the nuclear industry with the support for the application of advanced robotics to reduce nuclear power generating costs and enhance the safety of the personnel in the industry. The investigation included robotic and teleoperated systems. A robotic system is defined as a reprogrammable, multifunctional manipulator designed to move materials, parts, tools, or specialized devices through variable programmed motions for the performance of a variety of tasks. A teleoperated system includes an operator who remotely controls the system by direct viewing or through a vision system

  7. Nuclear fragmentation energy and momentum transfer distributions in relativistic heavy-ion collisions

    Science.gov (United States)

    Khandelwal, Govind S.; Khan, Ferdous

    1989-01-01

    An optical model description of energy and momentum transfer in relativistic heavy-ion collisions, based upon composite particle multiple scattering theory, is presented. Transverse and longitudinal momentum transfers to the projectile are shown to arise from the real and absorptive part of the optical potential, respectively. Comparisons of fragment momentum distribution observables with experiments are made and trends outlined based on our knowledge of the underlying nucleon-nucleon interaction. Corrections to the above calculations are discussed. Finally, use of the model as a tool for estimating collision impact parameters is indicated.

  8. Do we under-invest in nuclear energy research in the world? Lessons learned from a transferred macro-economic model

    International Nuclear Information System (INIS)

    The authors first present the Jones-and-Williams model which aims at assessing the socially optimal allowance ratio of R and D resources with respect to private allowance in order to see whether public power should 'dope' research. Then, they present the results obtained by transferring this model to nuclear electricity: values of estimated parameters, results of numerous simulations aimed at ensuring the model robustness. The influence of the main parameters (life duration, share of work in nuclear electricity production, global productivity of production factors in nuclear industry, substitution elasticity between production goods, and growth rate of nuclear R and D expenses) are graphically represented and discussed

  9. Effects of Scriptaid on Cell Cycle and Histone Acetylation of Ovine Nuclear Donor Cumulus Cells and their Ability to Support the Development of Somatic Cell Nuclear Transfer Embryos

    Directory of Open Access Journals (Sweden)

    Hui Cao

    2015-10-01

    Full Text Available Compelling evidence suggests that histone deacetylase inhibitor (HDACi influences the development of somatic cell nuclear transfer (SCNT embryos. The current study was conducted to determine the effect of pretreatment of donor cumulus cells with Scriptaid (a novel HDACi on cell cycle, histone acetylation and cloning embryos development in ovine. First, we optimized the efficiency of Scriptaid in a dose (0, 0.1, 0.2, 0.4 and 0.8 μmol/L and time-dependent (0, 12, 24, 36, and 48 h manner on the developmental capacity of these embryos. Then, we quantitatively assessed the alterations of acetylation levels in histone H3 lysine 9 (acH3K9 and histone H4 lysine 12 (acH4K12 of cumulus cells and SCNT embryos by immunofluorescence staining. Furthermore, we detected the proportion of G0/G1 phase cells in cumulus cells. We found a significantly improved blastocyst development rates of cloning embryos derived from donor cumulus cells pretreated with a mild dose (0.2 μmol/L of Scriptaid for 24 hours (21/86 [24.39%] vs. 11/85 [12.91%]; P<0.05. Meanwhile, the levels of acH3K9 and acH4K12 were also improved significantly in cumulus cells and SCNT embryos (P<0.05. Moreover, more cumulus cells pretreated with Scriptaid were in G0/G1 phase compared with control group (84.22% vs. 75.96%, P<0.05. In conclusion, donor cumulus cells treated with Scriptaid is beneficial to early development of SCNT embryos, ascending acH3K9/ acH4K12 and G0/G1 phase cells proportion of cumulus cell. Scriptaid can be used to improve the efficiency of somatic cell nuclear transfer in ovine.

  10. 78 FR 72979 - Bovine Spongiform Encephalopathy; Importation of Bovines and Bovine Products

    Science.gov (United States)

    2013-12-04

    ... risks of other livestock diseases, such as bovine viral diarrhea, foot-and-mouth disease, infectious... Products Derived from Bovines,'' published in the Federal Register on September 18, 2007 (72 FR 53314-53379... 92, 93, 94, et al. Bovine Spongiform Encephalopathy; Importation of Bovines and Bovine...

  11. Camel and bovine chymosin

    DEFF Research Database (Denmark)

    Jensen, Jesper Langholm; Mølgaard, Anne; Poulsen, Jens-Christian Navarro;

    2013-01-01

    Bovine and camel chymosin are aspartic peptidases that are used industrially in cheese production. They cleave the Phe105-Met106 bond of the milk protein κ-casein, releasing its predominantly negatively charged C-terminus, which leads to the separation of the milk into curds and whey. Despite...... having 85% sequence identity, camel chymosin shows a 70% higher milk-clotting activity than bovine chymosin towards bovine milk. The activities, structures, thermal stabilities and glycosylation patterns of bovine and camel chymosin obtained by fermentation in Aspergillus niger have been examined...... differential scanning calorimetry revealed a slightly higher thermal stability of camel chymosin compared with bovine chymosin. The crystal structure of a doubly glycosylated variant of camel chymosin was determined at a resolution of 1.6 Å and the crystal structure of unglycosylated bovine chymosin...

  12. Efeito do ibuprofeno administrado uma hora antes da inovulação de embriões bovinos Effect of ibuprofen administered one hour before the bovine embryo transfer

    Directory of Open Access Journals (Sweden)

    H.J. Narváez

    2010-06-01

    Full Text Available Avaliou-se o efeito do ibuprofeno administrado uma hora antes da inovulação de embriões bovinos, com o objetivo de melhorar a taxa de prenhez. Após a avaliação da resposta ao protocolo de sincronização do estro, 76 fêmeas selecionadas como receptoras de embriões foram distribuídas em três grupos (G experimentais: G1 (n=25 receptoras usadas como controle, G2 (n=30 receptoras que receberam ibuprofeno 5mg/kg, I.M, uma hora antes da inovulação dos embriões, e G3 (n=21 receptoras que receberam uma matriz polimérica de liberação controlada de ibuprofeno administrado por via subcutânea. As taxas de prenhez foram de 16% (4/25, 43,3% (13/30 e 14,2% (3/21, para G1, G2 e G3, respectivamente. Observou-se diferença (PThe effect of the administered ibuprofen was evaluated one hour before the embryo transfer of bovine embryos in order to improve pregnancy rates. After evaluating the response to protocol synchronization of estrus, 76 Females selected as the recipients of embryos were distributed into three experimental groups: G1 (n = 25 surrogate cows used as control, G2 (n = 30 surrogate cows that received 5mg/kg ibuprofen, IM, one hour before the embryo transfer, and G3 (n = 20 surrogate cows that received an array polymeric release of controlled ibuprofen subcutaneously administered. The pregnancy rates were 16% (4/25, 43.3% (13/30, and 14.2% (3/21 for G1, G2, and G3, respectively. There was statistical difference (P<0.024 on pregnancy rate of G2, in comparison with those of G1 and G3. The administration of ibuprofen intramuscularly one hour before the embryo transfer resulted in better pregnancy rate in Nellore surrogate cows.

  13. Experimental study on the heat transfer characteristics of a nuclear reactor containment wall cooled by gravitationally falling water

    Science.gov (United States)

    Pasek, Ari D.; Umar, Efrison; Suwono, Aryadi; Manalu, Reinhard E. E.

    2012-06-01

    Gravitationally falling water cooling is one of mechanism utilized by a modern nuclear Pressurized Water Reactor (PWR) for its Passive Containment Cooling System (PCCS). Since the cooling is closely related to the safety, water film cooling characteristics of the PCCS should be studied. This paper deals with the experimental study of laminar water film cooling on the containment model wall. The influences of water mass flow rate and wall heat rate on the heat transfer characteristic were studied. This research was started with design and assembly of a containment model equipped with the water cooling system, and calibration of all measurement devices. The containment model is a scaled down model of AP 1000 reactor. Below the containment steam is generated using electrical heaters. The steam heated the containment wall, and then the temperatures of the wall in several positions were measure transiently using thermocouples and data acquisition. The containment was then cooled by falling water sprayed from the top of the containment. The experiments were done for various wall heat rate and cooling water flow rate. The objective of the research is to find the temperature profile along the wall before and after the water cooling applied, prediction of the water film characteristic such as means velocity, thickness and their influence to the heat transfer coefficient. The result of the experiments shows that the wall temperatures significantly drop after being sprayed with water. The thickness of water film increases with increasing water flow rate and remained constant with increasing wall heat rate. The heat transfer coefficient decreases as film mass flow rate increase due to the increases of the film thickness which causes the increasing of the thermal resistance. The heat transfer coefficient increases slightly as the wall heat rate increases. The experimental results were then compared with previous theoretical studied.

  14. Multiparametric functional nuclear magnetic resonance imaging shows alterations associated with plasmid electro transfer in mouse skeletal muscle

    International Nuclear Information System (INIS)

    In vivo gene electro transfer is frequently used in preclinical gene therapy. Many studies have attempted to optimize protocols efficiency at the same time as reducing muscle damage. Most of them have reported histological evidence of muscle degeneration and completion of regeneration within 15 days. The functional consequences have rarely been addressed, which may reflect the lack of appropriate techniques. Yet, it is important to characterize the changes induced by the procedure itself because it may interfere with therapy. We used multiparametric functional (mpf)-nuclear magnetic resonance (NMR) imaging to evaluate mice hindlimb muscle after electro transfer of an empty plasmid. NMR experiments were performed in a 4 T Bruker magnet. Arterial spin labeling imaging of perfusion and blood oxygenation level dependent contrast and 31P spectroscopy of phosphocreatine kinetics and pH were simultaneously acquired from the mice hindlimb during 2 min of electrically stimulated exercise and recovery. After 15 days, hindlimb cross-sectional area decreased by 10% compared to control mice. Specific force-time integral and end-exercise pH were identical in both groups, whereas oxidative capacities increased. Perfusion values doubled, and oxygenation significantly decreased. Histology revealed: (i) degeneration/regeneration; (ii) a decrease in type IIb fibers and an increase in type I and IIa fibers; and (iii) increased capillary density. In this model, loss in muscle mass was accompanied by important alterations of perfusion and bio-energetics. Fifteen days after electro transfer, this was correlated with fiber type shift, capillary bed remodeling and degeneration/regeneration. mpf-NMR provides new insights into the functional consequences of standard electro transfer and represents a powerful tool for optimization and longitudinal assessment of preclinical gene therapy protocols. (authors)

  15. The French Training Programme for nuclear safety engineers, specially designed to transfer know-how to developing countries

    International Nuclear Information System (INIS)

    Agreements between the French Government and the developing countries entail a general commitment to transfer know-how. The need for nuclear safety training is often expressed. In addition, the expansion of the French nuclear programme has led to an increase in the staff of the national safety organizations. To meet these needs, the Nuclear Protection and Safety Institute (IPSN) has set up an original training programme which is open both to its own staff and to foreign participants. The programme is spread over a year and consists of an intensive study session of five weeks, a series of training programmes in the various departments of the Institute, and seminars at the end of the training period for all the trainees. At the end of the programme, each trainee presents to an examining panel the work he or she has performed in the last two or three training periods, which will normally relate to one specific area. The aims of the training programme are to give participants an overall view of safety problems with the accent on a particular type of nuclear facility, to enable participants to study in depth either the general practices or specific aspects of safety analysis, and particularly to become specialized in one aspect thereof, and to allow foreign participants access to the working methods used in France. The first training programme allowed us to examine how it operated. Lessons were drawn following an in-depth evaluation of the initial activities. A number of improvements were included in the second training programme. (author)

  16. High Power Nuclear Electric Propulsion (NEP) for Cargo and Propellant Transfer Missions in Cislunar Space

    Science.gov (United States)

    Falck, Robert D.; Borowski, Stanley K.

    2003-01-01

    The performance of Nuclear Electric Propulsion (NEP) in transporting cargo and propellant from Low Earth Orbit (LEO) to the first Earth-Moon Lagrange point (EML1) is examined. The baseline NEP vehicle utilizes a fission reactor system with Brayton power conversion for electric power generation to power multiple liquid hydrogen magnetoplasmadynamic (MPD) thrusters. Vehicle characteristics and performance levels are based on technology availability in a fifteen to twenty year timeframe. Results of numerical trajectory analyses are also provided.

  17. Production of rhesus monkey cloned embryos expressing monomeric red fluorescent protein by interspecies somatic cell nuclear transfer

    Energy Technology Data Exchange (ETDEWEB)

    Zhu, Hai-Ying; Kang, Jin-Dan; Li, Suo; Jin, Jun-Xue; Hong, Yu; Jin, Long; Guo, Qing; Gao, Qing-Shan; Yan, Chang-Guo; Yin, Xi-Jun, E-mail: yinxj33@msn.com

    2014-02-21

    Highlights: • Rhesus monkey cells were electroporated with a plasmid containing mRFP1, and an mRFP1-expressing cell line was generated. • For the first time, mRFP1-expressing rhesus monkey cells were used as donor cells for iSCNT. • The effect of VPA on the development of embryos cloned using iSCNT was determined. - Abstract: Interspecies somatic cell nuclear transfer (iSCNT) is a promising method to clone endangered animals from which oocytes are difficult to obtain. Monomeric red fluorescent protein 1 (mRFP1) is an excellent selection marker for transgenically modified cloned embryos during somatic cell nuclear transfer (SCNT). In this study, mRFP-expressing rhesus monkey cells or porcine cells were transferred into enucleated porcine oocytes to generate iSCNT and SCNT embryos, respectively. The development of these embryos was studied in vitro. The percentage of embryos that underwent cleavage did not significantly differ between iSCNT and SCNT embryos (P > 0.05; 71.53% vs. 80.30%). However, significantly fewer iSCNT embryos than SCNT embryos reached the blastocyst stage (2.04% vs. 10.19%, P < 0.05). Valproic acid was used in an attempt to increase the percentage of iSCNT embryos that developed to the blastocyst stage. However, the percentages of embryos that underwent cleavage and reached the blastocyst stage were similar between untreated iSCNT embryos and iSCNT embryos treated with 2 mM valproic acid for 24 h (72.12% vs. 70.83% and 2.67% vs. 2.35%, respectively). These data suggest that porcine-rhesus monkey interspecies embryos can be generated that efficiently express mRFP1. However, a significantly lower proportion of iSCNT embryos than SCNT embryos reach the blastocyst stage. Valproic acid does not increase the percentage of porcine-rhesus monkey iSCNT embryos that reach the blastocyst stage. The mechanisms underling nuclear reprogramming and epigenetic modifications in iSCNT need to be investigated further.

  18. Sensitizing solid state nuclear magnetic resonance of dilute nuclei by spin-diffusion assisted polarization transfers.

    Science.gov (United States)

    Lupulescu, Adonis; Frydman, Lucio

    2011-10-01

    Recent years have witnessed efforts geared at increasing the sensitivity of NMR experiments, by relying on the suitable tailoring and exploitation of relaxation phenomena. These efforts have included the use of paramagnetic agents, enhanced (1)H-(1)H incoherent and coherent transfers processes in 2D liquid state spectroscopy, and homonuclear (13)C-(13)C spin diffusion effects in labeled solids. The present study examines some of the opportunities that could open when exploiting spontaneous (1)H-(1)H spin-diffusion processes, to enhance relaxation and to improve the sensitivity of dilute nuclei in solid state NMR measurements. It is shown that polarization transfer experiments executed under sufficiently fast magic-angle-spinning conditions, enable a selective polarization of the dilute low-γ spins by their immediate neighboring protons. Repolarization of the latter can then occur during the time involved in monitoring the signal emitted by the low-γ nuclei. The basic features involved in the resulting approach, and its potential to improve the effective sensitivity of solid state NMR measurements on dilute nuclei, are analyzed. Experimental tests witness the advantages that could reside from utilizing this kind of approach over conventional cross-polarization processes. These measurements also highlight a number of limitations that will have to be overcome for transforming selective polarization transfers of this kind into analytical methods of choice.

  19. 78 FR 73993 - Bovine Spongiform Encephalopathy; Importation of Bovines and Bovine Products

    Science.gov (United States)

    2013-12-10

    ... Health Inspection Service 9 CFR Parts 92, 93, 94, 95, 96, and 98 RIN 0579-AC68 Bovine Spongiform Encephalopathy; Importation of Bovines and Bovine Products Corrections In rule document 2013-28228 appearing...

  20. 77 FR 20319 - Bovine Spongiform Encephalopathy; Importation of Bovines and Bovine Products

    Science.gov (United States)

    2012-04-04

    ...; ] DEPARTMENT OF AGRICULTURE Animal and Plant Health Inspection Service 9 CFR Part 93 RIN 0579-AC68 Bovine Spongiform Encephalopathy; Importation of Bovines and Bovine Products Correction In proposed rule...

  1. Participation of the national industry within a nuclear power plant program by technology transfer from the point of view of the main contractor

    International Nuclear Information System (INIS)

    The broad scope of components needed in a nuclear power plant with various technical requirements offer a big opportunity for the participation of local industries in the construction of such plants. Depending on the existing capability of the industrial enterprises, the scope of national participation can be increased by technology transfer on all technical areas to be applied for the construction of NPPs. Such technology requires as basis a nuclear program of the country determined and supported by the government and the utilities. This program has to be defined as realistic as even possible adjoined to the future energy demand of the country. Furthermore the available capability, existing qualifications and equipment of the national industry have to be considered. On the basis of these fundamental requirements a tailormade technology transfer program has to be elaborated in close cooperation with an experienced main contractor of the plant and his partners for such technology transfer and should be established afterwards. This program has to consider not only the goal to achieve finally the independent production of components and equipment for NPPs or the construction of complete power plant units itself, but also the economic benefit of such a program for the country. The costs of technology transfer and the necessary investment of the national industry required for the manufacture of nuclear components have to be thoroughly investigated, based on the expected scope of products to be manufactured for the nuclear power plants according to the nuclear program. Furthermore the application of the technology transferred for other components e.g. for conventional power stations, mineral-oil or chemical industrial plants has to be considered. By a tailormade nuclear technology transfer program, executed by qualified and experienced partners not only the quality of the products of the national industry for NPPs will be improved, but also the general standard regarding

  2. Effects of donor cells on in vitro development of cloned bovine embryos

    Institute of Scientific and Technical Information of China (English)

    Jing Fu; Pengfei Guan; Leiwen Zhao; Hua Li; Shuzhen Huang; Fanyi Zeng; Yitao Zeng

    2008-01-01

    The donor cells from different individuals and with different foreign genes introduced were investigated to determine their effects on the efficiency of somatic cell nuclear transfer (SCNT). The bovine ear fibroblast from different individuals was isolated, cultured, and then transfected with foreign genes to establish the stable cell lines, which were used as donor cells for nuclear transfer. The ooeytes were obtained through ovum pick up operation. After in vitro maturation, the M II phase oocytes were selected as receptors for nuclear transfer.The reconstructed embryos were cultured in vitro and observed at 2 h, 48 h, and 7 days after transfer to assess the rate of fusion using cleaved and blastoeyst as the parameters of SCNT efficiency. The donor cells from different individuals (04036, 06081, 06088, and 06129)had no obvious effect on the fusion and cleaved rate, whereas there was significant difference in the blastocyst rate (P0.05). It was concluded that the genetic background of the donor cells could affect the effi-ciency of SCNT, while the introduction of foreign genes into the donor cells had no obvious effect on the efficiency. This study provides useful information for the SCNT and would benefit in promoting the efficiency.

  3. Corrigendum to "Coupled thermochemical, isotopic evolution and heat transfer simulations in highly irradiated UO2 nuclear fuel"

    Science.gov (United States)

    Piro, M. H. A.; Banfield, J.; Clarno, K.; Simunovic, S.; Besmann, T. M.; Lewis, B. J.; Thompson, W. T.

    2016-09-01

    Figs. 7-9 in "Coupled thermochemical, isotopic evolution and heat transfer simulations in highly irradiated UO2 nuclear fuel" [1] have a consistent error corresponding to the relative proportions of iodine. Reported concentrations of iodine in the original manuscript are approximately ten times higher than expected, and are comparable in atomic proportions to cesium. One would expect that the amount of cesium would be about one order of magnitude greater than iodine based on the difference in fission yields of 235U and 239Pu. A practical consequence of this error would affect the predicted quantity and chemical composition of iodine on the fuel surface, which is related to iodine-induced stress corrosion cracking [2].

  4. The International Stripa Project: Technology transfer from cooperation in scientific and technological research on nuclear waste disposal

    International Nuclear Information System (INIS)

    The Nuclear Energy Agency of the organization for Economic Cooperation and Development (OECD/NEA) sponsors the International Stripa Project. The objectives of the Stripa Project are to develop techniques for characterizing sites located deep in rock formations that are potentially suitable for the geologic disposal of high-level radioactive wastes and to evaluate particular engineering design considerations that could enhance the long-term safety of a high-level radioactive waste repository in a geologic medium. The purpose of this paper is to briefly summarize the research conducted at Stripa and discuss the ways in which the technology developed for the Stripa Project has been and will be transfered to the United States Civilian Radioactive Waste Management Program's Yucca Mountain Project. 3 refs., 2 figs

  5. Effect of Nuclear Factor κB Inhibition on Serotype 9 Adeno-Associated Viral (AAV9) Minidystrophin Gene Transfer to the mdx Mouse

    OpenAIRE

    Reay, Daniel P.; Niizawa, Gabriela A; Watchko, Jon F; Daood, Molly; Reay, Ja’Nean C; Raggi, Eugene; Clemens, Paula R

    2012-01-01

    Gene therapy studies for Duchenne muscular dystrophy (DMD) have focused on viral vector-mediated gene transfer to provide therapeutic protein expression or treatment with drugs to limit dystrophic changes in muscle. The pathological activation of the nuclear factor (NF)-κB signaling pathway has emerged as an important cause of dystrophic muscle changes in muscular dystrophy. Furthermore, activation of NF-κB may inhibit gene transfer by promoting inflammation in response to the transgene or ve...

  6. Transfer of nuclear engineering knowledge at Hanoi University of Technology: Lessons learned and challenges

    International Nuclear Information System (INIS)

    Full text: Hanoi University of Technology (HUT) has been being the most important polytechnic education centre of the country for half a century. Nuclear Engineering Education Programme (NEEP) was started at HUT since the year 1970, right after establishment of Department of Nuclear Engineering at the University according to the initiative of the first Minister of Ministry of Higher Education of the country. Since the year 2000 the Department changed its education programme to adapt it to the actual circumstances in the country and renamed as Department of Nuclear Engineering and Environmental Physics (DONEEP). The objectives of the HUT's NEEP are as follows: 1. To train up nuclear technical manpower for development of peaceful uses of atomic energy in Vietnam. 2. To prepare initial nuclear technical human resources for introduction of Nuclear Power into the country. Aiming at these objectives, the Programme achieved remarkable results such as inestimable contributions to introducing and then developing the NDT radiography method in Vietnam, to improving and developing the atomic energy applications in the country, to providing important parts of technical human resources for strengthening the nuclear community of the country. The duration of 37 years of implementation of the Programme may be divided by 3 periods: from 1970 to 1989, 1990-2000 and from the year 2001 up to now. During the first period, the Programme was fully supported by the leadership of the University and the Ministry of Higher Education. The second period was full of difficulties. This was the period of searching the ways for preserving and adapting the Programme to the new circumstances in the country. The present period is the one of searching the ways for developing the NEEP at HUT. The lessons learned from 37-year implementation of the HUT's NEEP are as follows: 1. To establish proper objectives aiming to satisfy the urgent short term and/or long term demands of the country is the most

  7. Unlocking the bovine genome

    Science.gov (United States)

    The draft genome sequence of cattle (Bos taurus) has now been analyzed by the Bovine Genome Sequencing and Analysis Consortium and the Bovine HapMap Consortium, which together represent an extensive collaboration involving more than 300 scientists from 25 different countries. ...

  8. Development of the process of energy transfer from a nuclear Power Plant to an intermediate temperature electrolyse; Desarrollo del proceso de transferencia de energia desde una central nuclear a un electrolizador de temperatura intermedia

    Energy Technology Data Exchange (ETDEWEB)

    Munoz Cervantes, A.; Cuadrado Garcia, P.; Soraino Garcia, J.

    2013-07-01

    Fifty million tons of hydrogen are consumed annually in the world in various industrial processes. Among them, the ammonia production, oil refining and the production of methanol. One of the methods to produce it is the electrolysis of water, oxygen and hydrogen. This process needs electricity and steam which a central nuclear It can be your source; Hence the importance of developing the transfer process energy between the two. The objective of the study is to characterize the process of thermal energy transfer from a nuclear power plant to an electrolyzer of intermediate temperature (ITSE) already defined. The study is limited to the intermediate engineering process, from the central to the cell.

  9. Photoprotection and triplet energy transfer in higher plants: the role of electronic and nuclear fluctuations.

    Science.gov (United States)

    Cupellini, Lorenzo; Jurinovich, Sandro; Prandi, Ingrid G; Caprasecca, Stefano; Mennucci, Benedetta

    2016-04-20

    Photosynthetic organisms employ several photoprotection strategies to avoid damage due to the excess energy in high light conditions. Among these, quenching of triplet chlorophylls by neighboring carotenoids (Cars) is fundamental in preventing the formation of singlet oxygen. Cars are able to accept the triplets from chlorophylls by triplet energy transfer (TET). We have here studied TET rates in CP29, a minor light-harvesting complex (LHC) of the Photosystem II in plants. A fully atomistic strategy combining classical molecular dynamics of the LHC in its natural environment with a hybrid time-dependent density functional theory/polarizable MM description of the TET is used. We find that the structural fluctuations of the pigment-protein complex can largely enhance the transfer rates with respect to those predicted using the crystal structure, reducing the triplet quenching times in the subnanosecond scale. These findings add a new perspective for the interpretation of the photoprotection function and its relation with structural motions of the LHC. PMID:27052105

  10. Photoprotection and triplet energy transfer in higher plants: the role of electronic and nuclear fluctuations.

    Science.gov (United States)

    Cupellini, Lorenzo; Jurinovich, Sandro; Prandi, Ingrid G; Caprasecca, Stefano; Mennucci, Benedetta

    2016-04-28

    Photosynthetic organisms employ several photoprotection strategies to avoid damage due to the excess energy in high light conditions. Among these, quenching of triplet chlorophylls by neighboring carotenoids (Cars) is fundamental in preventing the formation of singlet oxygen. Cars are able to accept the triplets from chlorophylls by triplet energy transfer (TET). We have here studied TET rates in CP29, a minor light-harvesting complex (LHC) of the Photosystem II in plants. A fully atomistic strategy combining classical molecular dynamics of the LHC in its natural environment with a hybrid time-dependent density functional theory/polarizable MM description of the TET is used. We find that the structural fluctuations of the pigment-protein complex can largely enhance the transfer rates with respect to those predicted using the crystal structure, reducing the triplet quenching times in the subnanosecond scale. These findings add a new perspective for the interpretation of the photoprotection function and its relation with structural motions of the LHC.

  11. Communication received from the Permanent Mission of Sweden regarding the provision of certain additional information on production, inventories and international transfers of nuclear material and on exports of certain relevant equipment and non-nuclear material

    International Nuclear Information System (INIS)

    The document reproduces the text of a note verbale dated 28 June 1996 received by the Director General of IAEA from the Permanent Mission of Sweden through which the Government of Sweden provides, on a voluntary basis, certain additional information on production, inventories and international transfers of nuclear material and on exports of certain relevant equipment and non-nuclear material, in order to assist the Agency in the discharge of its safeguards responsibilities

  12. Communication received from the permanent mission of Finland regarding the provision of certain additional information on production, inventories and international transfers of nuclear material and on exports of certain relevant equipment and non-nuclear material

    International Nuclear Information System (INIS)

    The Director General received a note verbale of 8 February 1996 from Permanent Mission of Finland regarding the provision of certain additional information on production, inventories and international transfers of nuclear material and on exports of certain relevant equipment and non-nuclear material. In the light of the request expressed at the and of the note verbale, the text of the note verbale is being circulated

  13. Effects of different nuclear transfer and activation methods on the development of mouse somatic cell cloned embryos

    Institute of Scientific and Technical Information of China (English)

    Wang ErYao; YU Yang; Li XueMei; JIAO LiHong; Wang Liu

    2007-01-01

    A group of adult somatic cell cloned mice were obtained by using cumulus cells as nuclei donor cells. To study the effect of different nuclear transfer (NT) and activation methods on the development of mouse cloned embryos, embryos were reconstructed using two traditional NT methods (electrofusion and direct injection) and four activation treatments (electric pulse, ethanol, SrCl2 and electric pulse combined with SrCl2). The data showed that the efficiency of reconstruction using the direct injection method is significantly higher (90.7%) than that of the electrofusion method (49.7%). Parthenogenetic embryos can develop to blastocyst stage with three activation conditions, including ethanol, electric pulse and SrCl2; however, the rates of development to blastocyst after ethanol and electric pulse activation (52.4%, 54.2%) are significantly lower than after SrCl2 activation (76.9%). Treatment of embryos for 6 h with 10 mmol/L SrCl2 was found to be the best condition for activation of parthenogenetic as well as reconstructed embryos. By contrast, reconstructed embryos failed to develop to blastocyst stage after being activated by ethanol. The use of either injection or electrofusion for embryo reconstruction affected the pre-implantation development. However, after transfer in pseudopregnant mice, cloned mice were obtained from both methods.

  14. A Multi-Dimensional Heat Transfer Model of a Tie-Tube and Hexagonal Fuel Element for Nuclear Thermal Propulsion

    Science.gov (United States)

    Gomez, C. F.; Mireles, O. R.; Stewart, E.

    2016-01-01

    The Space Capable Cryogenic Thermal Engine (SCCTE) effort considers a nuclear thermal rocket design based around a Low-Enriched Uranium (LEU) design fission reactor. The reactor core is comprised of bundled hexagonal fuel elements that directly heat hydrogen for expansion in a thrust chamber and hexagonal tie-tubes that house zirconium hydride moderator mass for the purpose of thermalizing fast neutrons resulting from fission events. Created 3D steady state Hex fuel rod model with 1D flow channels. Hand Calculation were used to set up initial conditions for fluid flow. The Hex Fuel rod uses 1D flow paths to model the channels using empirical correlations for heat transfer in a pipe. Created a 2-D axisymmetric transient to steady state model using the CFD turbulent flow and Heat Transfer module in COMSOL. This model was developed to find and understand the hydrogen flow that might effect the thermal gradients axially and at the end of the tie tube where the flow turns and enters an annulus. The Hex fuel rod and Tie tube models were made based on requirements given to us by CSNR and the SCCTE team. The models helped simplify and understand the physics and assumptions. Using pipe correlations reduced the complexity of the 3-D fuel rod model and is numerically more stable and computationally more time-efficient compared to the CFD approach. The 2-D axisymmetric tie tube model can be used as a reference "Virtual test model" for comparing and improving 3-D Models.

  15. Development of computational technology on heat transfer and fluid flow in a nuclear fuel bundle of advanced reactor

    International Nuclear Information System (INIS)

    The assessment of the RANS(Reynolds-Averaged Navier-Stokes) based turbulence model was conducted to establish the optimal CFD system for turbulent flow and heat transfer in reactor during the first year of the project. The RANS models used in this project are the two-equation models based on the eddy viscosity assumption and the Second-Moment Closure(SMC) models. Since the nuclear fuel assembly loaded in the nuclear reactor is a rod bundle which is square or triangular array, the predictions using the various turbulence models were compared for turbulent flow in bare square and/or triangular rod bundle and the rod bundle with the flow mixing vane. The study for the second year of the project examined the CFD model and the applicability of the CFD code for the turbulent two-phase flow. The numerical predictions of lateral distributions of void fraction, phasic velocities and turbulent kinetic energy were compared against the experimental results for upward and downward bubbly flow in a vertical tube. The boiling flows in vertical tube and rod bundle were also simulated to verify the CFD results

  16. Effect of Embryo Transfer on the Bovine Estrus Synchronization and Conception%胚胎移植受体牛同期发情及受胎效果的研究

    Institute of Scientific and Technical Information of China (English)

    李刚

    2012-01-01

    The method of CIDR+E2 and twice use of uterine PGF2α infusion method were used in this study, embryo transfer for cattle estrus synchronization and fresh embryo transfer pregnancy was observed. The results showed that receptor bovine estrous rate at 24--48h was 85.5% and 51.6%, there was significantly difference between them (P〈0.05). Cattle before transplantation luteal receptor inspection pass rate was 79.3%, 77.8%, there was no significant difference (P〉0.05). Conception rate was 45.8% and 42.4%, the difference was not significant (P〉0.05). Estrus observed after 24h, 6.5d for direct examination Cattle with luteinizing for A, B grade can be used for transplantation.%本试验分别采用CIDR+E2法和两次PGF2α子宫输注法,对胚胎移植受体牛做同期发情处理,鲜胚移植观察其妊娠情况。试验结果表明两种处理方法的受体牛在24-48h发情率分别为85.5%和51.6%,两者之间差异显著(P〈0.05)。移植前对受体牛进行黄体检查,黄体合格率分另11为79.3%和77.8%,差异不显著(P〉0.05);受胎率分别为45.8%和42.4%,差异不显著(P〉0.05)。24h后观察发情,6.5d直检,黄体A、B级者用于移植。

  17. OECD/NEA Data Bank and nuclear science activities in support of knowledge preservation and transfer for innovation

    International Nuclear Information System (INIS)

    Full text: The OECD/Nuclear Energy Agency Data Bank was established as an institution for the collection, verification, validation, and dissemination of the basic tools used today for nuclear energy system design and simulation under different operating conditions. These tools comprise standardized databases with microscopic nuclear and chemical thermodynamic data, computer programs covering a wide range of applications, as well as information and data from integral experiments on fissile material systems, on reactor and radiation shielding mock-ups and on in-core fuel behaviour. They are continuously improved through user experience and feedback. The OECD/NEA Nuclear Science Committee (NSC) has identified the importance to establish international experiments databases, and is working in close collaboration with the Data Bank concerning the compilation, verification and dissemination of the compiled data. The main objective of this data preservation activity is to minimise the risk of losing highly valued knowledge developed in member countries, and to prevent additional costs to future generations should they reopen the issue of the development of similar nuclear systems. By fostering the current work, the Data Bank will become a center providing its members countries with a solid reference on which to base their future work. The collected information is preserved in an agreed standard format in computer accessible form. for use in the validation of current and new calculational schemes, for assessing uncertainties, confidence bounds and safety margins, and to record measurement methods and techniques. Training courses and workshops are mechanisms used for transferring the Data Bank's accumulated knowledge through world-wide participation of experts. The databases established so far cover the following areas: EXFOR and CINDA (experimental neutron-and charged particle induced reaction data and their bibliographic index with over 123,000 experiments from 70 years of

  18. [The radioecology of the grapevine. 1. The transfer of nuclear weapons fallout from the soil into wine].

    Science.gov (United States)

    Hellmuth, K H; Wagner, A; Fischer, E

    1989-04-01

    In a field investigation (1983-1985) comprising eight places of the most important viticultural regions in the Federal Republic of Germany, the contents of the radionuclides tritium (3H), carbon-14 (14C), strontium-90 (90Sr), and cesium-137 (137Cs) in air, soils, leaves of the vine, grapes and wine were measured and site-specific transfer factors were calculated. Data concerning soil parameters, climatic conditions, cultivation and vinification were collected. The tritium content of all samples was 10 Bq/l water of combustion, independent of location and year. The specific activity of 14C in the atmosphere and in biological material was 0.22 Bq/g carbon, independent of site and year. 90Sr contents of soils fluctuated between 0.7 and 3.5 Bq/kg dry matter. The mean content of leaves was 2 Bq/kg fresh material, of grapes 0.035 Bq/kg and of wine 0.008 Bq/l. 137Cs content of soils fluctuated between 1.3 and 7.9 Bq/kg dry matter. The mean content of leaves was 0.098 Bq/kg fresh material, of grapes 0.021 Bq/kg and of wine 0.0085 Bq/l. A relation between transfer of radionuclides and soil parameters and between the contents of grapes and wine was not recognizable. While cultivar-specific differences were not observed in grapes, red wines contained somewhat more 137Cs than white wines. Transfer factors soil grapes were 0.027 for 90Sr and 0.0057 for 137Cs. Site-specific influences such as soil parameters, climate, cultivation, vinification and differences between years led to a relatively small fluctuation of values. An influence of the nuclear power station Neckarwestheim has not been found in any of the radionuclides.

  19. Transference patron for the calibration of activemeters for use in nuclear medicine; Patron de transferencia para la calibracion de activimetros de uso en medicina nuclear

    Energy Technology Data Exchange (ETDEWEB)

    Cortes P, A.; Garcia D, O.C.; Ortiz P, I.; Becerril V, A. [Instituto nacional de Investigaciones Nucleares, Laboratorio de Patrones Radiactivos, Departamento de Metrologia, A.P. 18-1027. C.P. 11801 Mexico D.F. (Mexico)

    2000-07-01

    With the object to improve the calibration service of the activemetersof the users (nuclear medicine centres) so internal as external users at ININ and initiating the establishment of the traceability of the activity measures that carry out in the country, with respect to the National Patron of Nuclear Activity No. ININ-PNM-2, it is initiated the characterization and the performance control of the activemeter make Capintec, model CRC-7BT of the laboratory of Radioactive Patrons (LPR) of the Department of Metrology for to convert it in a transference patron. This characterization and control are based in the sustained review of the activemeter calibration for the I-131, Mo-99 and Sm-153 radioisotopes with activities from 100 mCi until some {mu} Ci and the Tc-99m, In-111, Tl-201, Ga-67, Cs-137, Co-60, and Ba-133 with activities of the range of some {mu} Ci. To verify the good performance of the instrument it was revised the linearity of its scale, the stability of readings, the variation of readings respect of the source position inside the activemeter well and it was determined the calibration factors for each one of the radiosotopes mentionated. The radioactive sources of mid-short life that are used which consist in radioactive solutions of 10 cm{sup 3} contained in polyethylene small bottles of 25 cm{sup 3} elaborated in the LPR and whose activity is measured with respect to the National Patron ININ-PNM-2. The sources of mid-large life are ampoules with 5 cm{sup 3} of radioactive solution, calibrated in activity by the primary laboratory, LNHB (formerly LMRI) of France. (Author)

  20. The total cross sections of heavy ion reaction and the nuclear transfer

    International Nuclear Information System (INIS)

    The total reaction cross section of the systems 12C + 12C, 12C + 40Ca, 12C + 90Zr, 12C + 208Pb, 40Ca + 40Ca, 40Ca + 208Pb + 90Zr + 90Zr, 90Zr + 208Pb and 208Pb + 208Pb for a wide range of energies has been calculated microscopically. A WKB expression for the imaginary phase shift in the impact paremeter representation has been employed. The imaginary part of the optical potential has been constructed by using the first term of multiple scattering theory with the effect of Pauli blocking incorporated into it. The inclusion of the nuclear and Coulomb interactions is shown to be important. The theoretical results do not show very good agreement with the experimental data at lower energies. This is attributed to the weak absorption contained in the imaginary potential of the ''tοA οB'' interaction, wich contained only quasifree knock-out as the dominant reaction mechanism. (author)

  1. Nuclear effects in deep inelastic lepton nucleon scattering at different momentum transfers

    International Nuclear Information System (INIS)

    The ratio of the structure function F2sup(A)(x) of a nucleus A to that of deuterium F2sup(D)(x)is computed for A = Fe, Al; 12C and 4He. The difference between lepton-nucleus and lepton-nucleon scattering is explained by a fraction g of the valence quarks of nuclear matter moving effectively in bags of doubled size (i.e. α's). The difference between EMC and SLAC data stems from the different effective quark mass, which is close to zero at -q2 = 100 GeV2, but (140 +- 25) MeV, at 2> = 3 GeV2. A cluster probability of g = 15% (10%) fits the Fe (Al) data well; g = 1/3 is predicted for 4He (and 12C) on grounds of the Barshay three gluon force. (orig.)

  2. High in vitro development after somatic cell nuclear transfer and trichostatin A treatment of reconstructed porcine embryos

    DEFF Research Database (Denmark)

    Li, J.; Østrup, Olga; Villemoes, Klaus;

    2008-01-01

    Abnormal epigenetic modification is supposed to be one of factors accounting for inefficient reprogramming of the donor cell nuclei in ooplasm after somatic cell nuclear transfer (SCNT). Trichostatin A (TSA) is an inhibitor of histone deacetylase, potentially enhancing cloning efficiency. The aim...

  3. DNA methylation in porcine preimplantation embryos developed in vivo and produced by in vitro fertilization, parthenogenetic activation and somatic cell nuclear transfer

    DEFF Research Database (Denmark)

    Deshmukh, Rahul Shahaji; Østrup, Olga; Østrup, Esben;

    2011-01-01

    DNA demethylation and remethylation are crucial for reprogramming of the differentiated parental/somatic genome in the recipient ooplasm upon somatic cell nuclear transfer. Here, we analyzed the DNA methylation dynamics during porcine preimplantation development. Porcine in vivo developed (IV), i...

  4. Comparative molecular analysis of ovine and bovine Streptococcus uberis isolates.

    Science.gov (United States)

    Gilchrist, T L; Smith, D G E; Fitzpatrick, J L; Zadoks, R N; Fontaine, M C

    2013-02-01

    Streptococcus uberis causes clinical and subclinical mastitis in cattle and sheep, but it is unknown whether the composition of Strep. uberis populations differs between host species. To address this, we characterized a collection of bovine and ovine Strep. uberis isolates with shared geographical and temporal origins by means of an expanded multilocus sequence typing scheme. Among 14 ovine and 35 bovine isolates, 35 allelic profiles were detected. Each allelic profile was associated with a single host species and all but one were new to the multilocus sequence typing database. The median number of new alleles per isolate was higher for ovine isolates than for bovine isolates. None of the ovine isolates belonged to the global clonal complexes 5 or 143, which are commonly associated with bovine mastitis and which have a wide geographical distribution. Ovine isolates also differed from bovine isolates in carriage of plasminogen activator genes, with significantly higher prevalence of pauB in ovine isolates. Isolates that were negative for yqiL, one of the targets of multilocus sequence typing, were found among ovine and bovine isolates and were not associated with a specific sequence type or global clonal complex. One bovine isolate carried a gapC allele that was probably acquired through lateral gene transfer, most likely from Streptococcus salivarius. We conclude that ovine isolates are distinct from bovine isolates of Strep. uberis, and that recombination between isolates from different host species or bacterial species could contribute to changes in virulence gene profiles with relevance for vaccine development. PMID:23200465

  5. Bovine Herpesvirus 4 infections and bovine mastitis

    NARCIS (Netherlands)

    Wellenberg, Gerardus Johannus

    2002-01-01

    Mastitis is an often occurring disease in dairy cattle with an enormous economic impact for milk producers worldwide. Despite intensive research, which is historically based on the detection of bacterial udder pathogens, still around 20-35% of clinical cases of bovine mastitis have an unknown aetiol

  6. Generation of bovine (Bos indicus) and buffalo (Bubalus bubalis) adipose tissue derived stem cells: isolation, characterization, and multipotentiality.

    Science.gov (United States)

    Sampaio, R V; Chiaratti, M R; Santos, D C N; Bressan, F F; Sangalli, J R; Sá, A L A; Silva, T V G; Costa, N N; Cordeiro, M S; Santos, S S D; Ambrosio, C E; Adona, P R; Meirelles, F V; Miranda, M S; Ohashi, O M

    2015-01-15

    Adult stem cells are known for their plasticity and their potential to differentiate into several different cell types; these characteristics have implications for cell therapy and reproductive biotechnologies. In this study, we report on the isolation and characterization of mesenchymal stem cells (MSC) derived from bovine and buffalo adipose tissue. Cells isolated using enzymatic digestion of bovine and buffalo adipose-tissue biopsy samples were grown in vitro for at least 15 passages, verifying their capacity to proliferate. These cells were also subjected to immunophenotypic characterization for the presence of CD90, CD105, and CD79, and the absence of CD45, CD34, and CD73, which are positive and negative markers of MSC, respectively. To prove their multipotency, the cells were induced to differentiate into three different cell types, chondrocytes, osteoblasts, and adipocytes, which were stained with tissue-specific dyes (Chondrogenic-Alcian Blue, Osteogenic-Alizarin Red, and Adipogenic-Oil-Red O, respectively) to confirm differentiation. Gene expression analysis of pluripotency-related genes was also conducted. Our results suggest that adipose tissue from bovines and buffalos can be used as a source of MSC, making adipose tissue-derived cells an interesting option for cell therapy and regenerative medicine. Additionally, these findings have implications for reproductive biotechnology because the use of MSC as nuclear donors has been linked to an increase in the efficiency of nuclear transfer.

  7. Targeting exogenous GDNF gene to the bovine somatic cell beta-casein locus for the production of transgenic bovine animals.

    Science.gov (United States)

    Zhang, X M; Luo, F H; Ding, H M; Li, B; Zhang, J J; Wu, Y J

    2015-01-01

    Considerable attention is currently being directed toward methods for producing recombinant human proteins in the mammary glands of genetically modified transgenic livestock. However, the expression of inserted genes in transgenic animals is variable and often very low because of the randomness of the site of transgene integration. One possible strategy to avoid the expression problem associated with random integration is to use site-specific integration by targeting integration to a high expression locus and, thereby, to improve expression of the transferred gene. In the present study, we focused on glial cell line-derived neurotrophic factor (GDNF), a novel type of neurotrophic factor first cloned in 1993. Research has shown that GDNF may have potential applications in the treatment of Parkinson's disease and other diseases of the central nervous system since it acts as a protective factor for central dopaminergic neurons. Here, we constructed a gene targeting vector to knock-in the human GDNF gene at the bovine beta-casein gene locus as a first step to producing transgenic animals with a high level of expression of human GDNF protein in their mammary glands. Bovine fetal fibroblast cells were transfected with linearized pNRTCNbG by electroporation. Three cell clones were identified with successful targeting to the beta-casein locus; and were confirmed using both polymerase chain reaction analysis and sequencing. Gene-targeted cells were used as nuclear donors; a total of 161 embryos were reconstructed, 23 of which developed to the blastocyst stage. These blastocysts were transferred to 8 recipient cows, but no offspring were obtained. PMID:26634460

  8. Hyperpolarization of 29Si by Resonant Nuclear Spin Transfer from Optically Hyperpolarized 31P Donors

    Science.gov (United States)

    Dluhy, Phillip; Salvail, Jeff; Saeedi, Kamyar; Thewalt, Mike; Simons, Stephanie

    2014-03-01

    Recent developments in nanomedicine have allowed nanoparticles of silicon containing hyperpolarized 29Si to be imaged in vivo using magnetic resonance imaging. The extremely long relaxation times and isotropy of the Si lattice make polarized 29Si isotopes ideal for these sorts of imaging methods. However, one of the major difficulties standing in the path of widespread adoption of these techniques is the slow rate at which the 29Si is hyperpolarized and the limited maximum hyperpolarization achievable. In this talk, I will describe an effective method for hyperpolarization of the 29Si isotopes using resonant optical pumping of the donor bound exciton transitions to polarize the 31P donor nuclei, and a choice of static magnetic field that conserves energy during spin flip flops between donor nuclear and 29Si spins to facilitate diffusion of this polarization. Using this method, we are able to polarize greater than 10% of the 29Si centers in 64 hours without seeing saturation of the 29Si polarization.

  9. Production of human lysozyme-transgenic cloned porcine embryos by somatic nuclear transfer

    Institute of Scientific and Technical Information of China (English)

    Qiuyan Li; Hengxi Wei; Ying Guo; Yan Li; Rui Zhao; Yufang Ma; Zhengquan Yu; Bo Tang; Lei Zhang; Yunping Dai; Ning Li

    2009-01-01

    Due to their physiology and organ size, pigs have significant potential as human disease models and as organ transplantation donors. Genetic modification of pigs could provide benefits for both agriculture and human medicine. In this study, five fetal pig fibroblast cell lines from two species (Wuzhishan and Landrace pigs) were transfected using double-marked human lysozyme (HLY) plasmids (pBC1-HLY-GFP-NEO) by a liposome-mediated method. The ratio of green fluorescent protein (GFP)-expressing cells was >95% in sw7, sw8, s1w3 and s1w6 cell lines, but only 49.3% in slw9 cells. Cells from the four highly transgenic lines were used as nuclear donors to construct embryos, which were then cultured after fusion and activation by electric stimulation. The rate of cleavage was 76.7%, 48 h after acti-vation. After 7 days, 18.5% of cleaved eggs had developed to the blastocyst stage and 93.3% of blastocysts were GFP-positive. These results indicate that transgenic fetal pig fibroblast cell lines could be obtained by a liposome-mediated method, though the transfection efficiency varied between cell lines. Reconstructed embryos derived from transgenic cells could successfully develop into blastocysts, most of which were GFP-positive.

  10. Nuclear

    International Nuclear Information System (INIS)

    This document proposes a presentation and discussion of the main notions, issues, principles, or characteristics related to nuclear energy: radioactivity (presence in the environment, explanation, measurement, periods and activities, low doses, applications), fuel cycle (front end, mining and ore concentration, refining and conversion, fuel fabrication, in the reactor, back end with reprocessing and recycling, transport), the future of the thorium-based fuel cycle (motivations, benefits and drawbacks), nuclear reactors (principles of fission reactors, reactor types, PWR reactors, BWR, heavy-water reactor, high temperature reactor of HTR, future reactors), nuclear wastes (classification, packaging and storage, legal aspects, vitrification, choice of a deep storage option, quantities and costs, foreign practices), radioactive releases of nuclear installations (main released radio-elements, radioactive releases by nuclear reactors and by La Hague plant, gaseous and liquid effluents, impact of releases, regulation), the OSPAR Convention, management and safety of nuclear activities (from control to quality insurance, to quality management and to sustainable development), national safety bodies (mission, means, organisation and activities of ASN, IRSN, HCTISN), international bodies, nuclear and medicine (applications of radioactivity, medical imagery, radiotherapy, doses in nuclear medicine, implementation, the accident in Epinal), nuclear and R and D (past R and D programmes and expenses, main actors in France and present funding, main R and D axis, international cooperation)

  11. Heat Transfer Salts for Nuclear Reactor Systems - Chemistry Control, Corrosion Mitigation, and Modeling

    Energy Technology Data Exchange (ETDEWEB)

    Anderson, Mark [Univ. of Wisconsin, Madison, WI (United States); Sridharan, Kumar [Univ. of Wisconsin, Madison, WI (United States); Morgan, Dane [Univ. of Wisconsin, Madison, WI (United States); Peterson, Per [Univ. of Wisconsin, Madison, WI (United States); Calderoni, Pattrick [Univ. of Wisconsin, Madison, WI (United States); Scheele, Randall [Univ. of Wisconsin, Madison, WI (United States); Casekka, Andrew [Univ. of Wisconsin, Madison, WI (United States); McNamara, Bruce [Univ. of Wisconsin, Madison, WI (United States)

    2015-01-22

    The concept of a molten salt reactor has existed for nearly sixty years. Previously all work was done during a large collaborative effort at Oak Ridge National Laboratory, culminating in a research reactor which operated for 15,000 hours without major error. This technical success has garnished interest in modern, high temperature, reactor schemes. Research using molten fluoride salts for nuclear applications requires a steady supply of high grade molten salts. There is no bulk supplier of research grade fluoride salts in the world, so a facility which could provide all the salt needed for testing at the University of Wisconsin had to be produced. Two salt purification devices were made for this purpose, a large scale purifier, and a small scale purifier, each designed to clean the salts from impurities and reduce their corrosion potential. As of now, the small scale has performed with flibe salt, hydrogen, and hydrogen fluoride, yielding clean salt. This salt is currently being used in corrosion testing facilities at the Massachusetts Institute of Technology and the University of Wisconsin. Working with the beryllium based salts requires extensive safety measures and health monitoring to prevent the development of acute or chronic beryllium disease, two pulmonary diseases created by an allergic reaction to beryllium in the lungs. Extensive health monitoring, engineering controls, and environment monitoring had to be set up with the University of Wisconsin department of Environment, Health and Safety. The hydrogen fluoride required for purification was also an extreme health hazard requiring thoughtful planning and execution. These dangers have made research a slow and tedious process. Simple processes, such as chemical handling and clean-up, can take large amounts of ingenuity and time. Other work has complemented the experimental research at Wisconsin to advance high temperature reactor goals. Modeling work has been performed in house to re

  12. Economic optimization of the number of recipients in bovine embryo transfer programs Otimização econômica do número de receptoras em programas de transferência de embriões em bovinos

    Directory of Open Access Journals (Sweden)

    Renato Travassos Beltrame

    2007-06-01

    Full Text Available Purchase and maintenance of recipient females account for a large proportion of the costs and determine the number of calves that can be produced in an embryo transfer program. However, the large variability of embryo production by the donors and the need to purchase and synchronize the recipients before knowing the number of embryos collected make it difficult for the decision maker to identify the ideal number of recipient females to allocate. An ex-ante evaluation to determine the optimal number of recipient females was carried out through a sensitivity analysis for the ratio between the number of recipients and donors in a simulation model. The variability for the number of embryos collected was accounted for by applying the Monte Carlo simulation technique, assuming normal distribution and known values for mean and variance. The simulation considered monthly intervals between collections, during a 24 months program. The effect of embryo freezing on the number of pregnancies was considered by introducing a stock of frozen embryos into the mathematical model. Optimal recipient/donor ratio and the cost per pregnancy were compared for three recipient synchronization protocols (prostaglandin, progesterone - P4 and Ovsynch, based on the expected performance for synchronization, conception and transfer/treated rates for each protocol. Stochastic simulation associated with sensitivity analysis was effective in identifying the optimal donor to recipient ratio. Freezing embryos is effective to reduce the operational costs per pregnancy. The estimated optimal recipient/donor ratio was 20 for prostaglandin and 16.7 for the other protocols. The P4 protocol, although the most expensive, resulted in the lowest pregnancy cost estimation followed by prostaglandin and Ovsynch.A aquisição e manutenção de receptoras representam grande proporção dos custos e determinam o número de produtos gerados em um programa de transferência de embriões. Entretanto

  13. Developmental disparity between in vitro-produced and somatic cell nuclear transfer bovine days 14 and 21 embryos

    DEFF Research Database (Denmark)

    Alexopoulos, Natalie I.; Maddox-Hyttel, Poul; Tveden-Nyborg, Pernille Yde;

    2008-01-01

    , immunohistochemistry, and transmission electron microscopy to establish in vivo developmental milestones. Following morphological examination, samples were characterized for the presence of epiblast (POU5F1), mesoderm (VIM), and neuroectoderm (TUBB3). On D14, only 25, 15, and 7% of IVP, SUZI, and HMC embryos were...... of VIM and TUBB3 was less distinct in SCNT embryos when compared with IVP embryos, indicating slower or compromised development. In conclusion, SCNT and to some degree, IVP embryos displayed a high rate of embryonic mortality before D14 and surviving embryos displayed reduced quality with respect...

  14. Spindle formation and microtubule organization during first division in reconstructed rat embryos produced by somatic cell nuclear transfer.

    Science.gov (United States)

    Tomioka, Ikuo; Mizutani, Eiji; Yoshida, Tomoyuki; Sugawara, Atsushi; Inai, Kentaro; Sasada, Hiroshi; Sato, Eimei

    2007-08-01

    The present study was conducted to demonstrate the spindle formation and behavior of chromosomes and microtubules during first division in reconstructed rat embryos produced by somatic cell nuclear transfer (SCNT) with cumulus cell nuclei. To demonstrate the effect of oocyte aging after ovulation on the cleavage of SCNT embryos, micromanipulation was carried out 11, 15 and 18 h after injection of hCG. SCNT oocytes were activated by incubation in culture medium supplemented with 5 microM ionomycin for 5 min followed by treatment with 2 mM 6-dimethylaminopurine (6-DMAP) in mR1ECM for 2-3 h. For immunocytochemical observation, the SCNT embryos were incubated with monoclonal anti-alpha-tubulin antibody and then fluorescein isothiocyanate (FITC)-conjugated goat anti-mouse IgG. Cleavage rates were significantly higher for oocytes collected after 15 and 18 h rather than for those collected 11 h after injection of hCG (56 and 53%, respectively vs. 28%; P<0.05). Premature chromosome condensation occurred before activation of the SCNT oocytes, but adequate spindle formation was only rarely observed. The distribution of microtubules in SCNT embryos after activation was different from those of fertilized and parthenogenic oocytes, i.e., a dense microtubule organization shaped like a ring was observed. Eighteen to 20 h post-activation, most SCNT embryos were in the 2-cell stage, but no nucleoli were clearly visible, which was quite different from the fertilized oocytes. In addition, first division with and without small cellular bodies containing DNA was observed in the rat SCNT embryos in some cases. The present study suggests that reorganization of transferred nuclei in rat SCNT embryos may be inadequate in terms of formation of the mitotic assembly and nucleolar reorganization. PMID:17446658

  15. Differences during the first lactation between cows cloned by somatic cell nuclear transfer and noncloned cows.

    Science.gov (United States)

    Montazer-Torbati, F; Boutinaud, M; Brun, N; Richard, C; Neveu, A; Jaffrézic, F; Laloë, D; LeBourhis, D; Nguyen, M; Chadi, S; Jammes, H; Renard, J-P; Chat, S; Boukadiri, A; Devinoy, E

    2016-06-01

    Lactation performance is dependent on both the genetic characteristics and the environmental conditions surrounding lactating cows. However, individual variations can still be observed within a given breed under similar environmental conditions. The role of the environment between birth and lactation could be better appreciated in cloned cows, which are presumed to be genetically identical, but differences in lactation performance between cloned and noncloned cows first need to be clearly evaluated. Conflicting results have been described in the literature, so our aim was to clarify this situation. Nine cloned Prim' Holstein cows were produced by the transfer of nuclei from a single fibroblast cell line after cell fusion with enucleated oocytes. The cloned cows and 9 noncloned counterparts were raised under similar conditions. Milk production and composition were recorded monthly from calving until 200d in milk. At 67d in milk, biopsies were sampled from the rear quarter of the udder, their mammary epithelial cell content was evaluated, and mammary cell renewal, RNA, and DNA were then analyzed in relevant samples. The results showed that milk production did not differ significantly between cloned and noncloned cows, but milk protein and fat contents were less variable in cloned cows. Furthermore, milk fat yield and contents were lower in cloned cows during early lactation. At around 67 DIM, milk fat and protein yields, as well as milk fat, protein, and lactose contents, were also lower in cloned cows. These lower yields could be linked to the higher apoptotic rate observed in cloned cows. Apoptosis is triggered by insulin-like factor growth binding protein 5 (IGFBP5) and plasminogen activator inhibitor (PAI), which both interact with CSN1S2. During our experiments, CSN1S2 transcript levels were lower in the mammary gland of cloned cows. The mammary cell apoptotic rate observed in cloned cows may have been related to the higher levels of DNA (cytosine-5

  16. Aberrant DNA methylation in 5'regions of DNA methyltransferase genes in aborted bovine clones

    Institute of Scientific and Technical Information of China (English)

    2008-01-01

    High rate of abortion and developmental abnormalities is thought to be closely associated with inefficient epigenetic reprogramming of the transplanted nuclei during bovine cloning.It is known that one of the important mechanisms for epigenetic reprogramming is DNA methylation.DNA methylation is established and maintained by DNA methyltransferases(DNMTs),therefore,it is postulated that the inefficient epigenetic reprogramming of transplanted nuclei may be due to abnormal expression of DNMTs.Since DNA methylation can strongly inhibit gene expression,aberrant DNA methylation of DNMT genes may disturb gene expression.But presently,it is not clear whether the methylation abnormality of DNMT genes is related to developmental failure of somatic cell nuclear transfer embryos.In our study,we analyzed methylation patterns of the 5' regions of four DNMT genes including Dnmt3a,Dnmt3b,Dnmtl and Dnmt2 in four aborted bovine clones.Using bisulfite sequencing method,we found that 3 out of 4 aborted bovine clones(AF1,AF2 and AF3)showed either hypermethylation or hypomethylation in the 5' regions of Dnmt3a and Dnmt3b.indicating that Dnmt3a and Dnmt3b genes are not properly reprogrammed.However,the individual AF4 exhibited similar methylation level and pattern to age-matched in vitro fertilized (IVF)fetuses.Besides,we found that tle 5'regions of Dnmtl and Dnmt2 were nearly completely unmethylated in all normal adults.IVF fetuses,sperm and aborted clones.Together,our results suggest that the aberrant methylation of Dnmt3a and Dnmt3b 5' regions is probably associated with the high abortion of bovine clones.

  17. Interspecies nuclear transfer using fibroblasts from leopard, tiger, and lion ear piece collected postmortem as donor cells and rabbit oocytes as recipients.

    Science.gov (United States)

    Yelisetti, Uma Mahesh; Komjeti, Suman; Katari, Venu Charan; Sisinthy, Shivaji; Brahmasani, Sambasiva Rao

    2016-06-01

    Skin fibroblast cells were obtained from a small piece of an ear of leopard, lion, and tiger collected postmortem and attempts were made to synchronize the skin fibroblasts at G0/G1 of cell cycle using three different approaches. Efficiency of the approaches was tested following interspecies nuclear transfer with rabbit oocytes as recipient cytoplasm. Fluorescence-activated cell sorting revealed that the proportion of G0/G1 cells increased significantly (P sodium butyrate (NaBu) treatment when compared with cycling cells. However, 3 mM NaBu treatment caused alterations in cell morphology and increase in dead cells. Thus, interspecies nuclear transfer was carried out using fibroblast cells subjected to contact inhibition for 72 h, serum starvation for 48 h, and cells treated with 1.0 mM NaBu for 48 h. The fusion rates, the proportion of fused couplets that cleaved to two-cell and developed to blastocyst, were highest in all three species when the donor cells were treated with 1.0 mM NaBu for 48 h. But, the blastocyst percentage of interspecies nuclear embryos (5-6%) was significantly lower when compared with rabbit-rabbit nuclear transfer embryos (22.9%). In conclusion, fibroblast cells of leopard, lion, and tiger were successfully synchronized and used for the development of blastocysts using rabbit oocytes as recipient cytoplasm. PMID:27071624

  18. Generation of porcine fetal fibroblasts expressing the tetracycline-inducible Cas9 gene by somatic cell nuclear transfer.

    Science.gov (United States)

    Liu, Guoqian; Liu, Kai; Wei, Hengxi; Li, Li; Zhang, Shouquan

    2016-09-01

    Cas9 endonuclease, from so-called clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated (Cas) systems of Streptococcus pyogenes, type II functions as an RNA-guided endonuclease and edits the genomes of prokaryotic and eukaryotic organisms, including deletion and insertion by DNA double‑stranded break repair mechanisms. In previous studies, it was observed that Cas9, with a genome‑scale lentiviral single‑guide RNA library, could be applied to a loss‑of‑function genetic screen, although the loss‑of‑function genes have yet to be verified in vitro and this approach has not been used in porcine cells. Based on these observations, lentiviral Cas9 was used to infect porcine primary fibroblasts to achieve cell colonies carrying Cas9 endonuclease. Subsequently, porcine fetal fibroblasts expressing the tetracycline‑inducible Cas9 gene were generated by somatic cell nuclear transfer, and three 30 day transgenic porcine fetal fibroblasts (PFFs) were obtained. Polymerase chain reaction (PCR), reverse transcription‑PCR and western blot analysis indicated that the PFFs were Cas9‑positive. In addition, one of the three integrations was located near to known functional genes in the PFF1 cell line, whereas neither of the integrations was located in the PFF1 or PFF2 cell lines. It was hypothesized that these transgenic PFFs may be useful for conditional genomic editing in pigs, and for generating ideal modified porcine models. PMID:27430306

  19. Cloning adult farm animals: a review of the possibilities and problems associated with somatic cell nuclear transfer.

    Science.gov (United States)

    Edwards, J L; Schrick, F N; McCracken, M D; van Amstel, S R; Hopkins, F M; Welborn, M G; Davies, C J

    2003-08-01

    In 1997, Wilmut et al. announced the birth of Dolly, the first ever clone of an adult animal. To date, adult sheep, goats, cattle, mice, pigs, cats and rabbits have been cloned using somatic cell nuclear transfer. The ultimate challenge of cloning procedures is to reprogram the somatic cell nucleus for development of the early embryo. The cell type of choice for reprogramming the somatic nucleus is an enucleated oocyte. Given that somatic cells are easily obtained from adult animals, cultured in the laboratory and then genetically modified, cloning procedures are ideal for introducing specific genetic modifications in farm animals. Genetic modification of farm animals provides a means of studying genes involved in a variety of biological systems and disease processes. Moreover, genetically modified farm animals have created a new form of 'pharming' whereby farm animals serve as bioreactors for production of pharmaceuticals or organ donors. A major limitation of cloning procedures is the extreme inefficiency for producing live offspring. Dolly was the only live offspring produced after 277 attempts. Similar inefficiencies for cloning adult animals of other species have been described by others. Many factors related to cloning procedures and culture environment contribute to the death of clones, both in the embryonic and fetal periods as well as during neonatal life. Extreme inefficiencies of this magnitude, along with the fact that death of the surrogate may occur, continue to raise great concerns with cloning humans. PMID:12846674

  20. An ideal oocyte activation protocol and embryo culture conditions for somatic cell nuclear transfer using sheep oocytes.

    Science.gov (United States)

    Patel, Hiren; Chougule, Shruti; Chohan, Parul; Shah, Naval; Bhartiya, Deepa

    2014-10-01

    Pluripotent stem cells are possibly the best candidates for regenerative medicine, and somatic cell nuclear transfer (SCNT) is one of the viable options to make patient-specific embryonic stem cells. Till date efficacy of SCNT embryos is very low and requires further improvement like ideal oocyte activation and in vitro culture system. The aim of the present study was to evaluate ideal oocyte activation using different stimulation protocols and to study the effect of cumulus co-culture conditions on embryo development. Results demonstrate that between electric stimulation and chemical stimulation using calcium ionomycin and ionophore, best oocyte activation was obtained using calcium ionomycin (5 microM for 5 min) which resulted in 83% cleavage followed by 7% of early blastocyst which further increased to 15% when a cumulus bed was also introduced during embryo culture. Sequential modified Charles Rosenkrans 2 (mCR2) medium was used for embryo culture in which glucose levels were increased from 1 mM to 5 mM from Day 3 onwards. SCNT using cumulus cells as donor somatic cell, calcium ionomycin to activate the reconstructed oocyte and embryo culture on a cumulus bed in sequential mCR2 medium, resulted in the development of 6% embryos to early blastocyst stage. Such technological advances will make SCNT a viable option to make patient-specific pluripotent stem cell lines in near future.

  1. Cloning adult farm animals: a review of the possibilities and problems associated with somatic cell nuclear transfer.

    Science.gov (United States)

    Edwards, J L; Schrick, F N; McCracken, M D; van Amstel, S R; Hopkins, F M; Welborn, M G; Davies, C J

    2003-08-01

    In 1997, Wilmut et al. announced the birth of Dolly, the first ever clone of an adult animal. To date, adult sheep, goats, cattle, mice, pigs, cats and rabbits have been cloned using somatic cell nuclear transfer. The ultimate challenge of cloning procedures is to reprogram the somatic cell nucleus for development of the early embryo. The cell type of choice for reprogramming the somatic nucleus is an enucleated oocyte. Given that somatic cells are easily obtained from adult animals, cultured in the laboratory and then genetically modified, cloning procedures are ideal for introducing specific genetic modifications in farm animals. Genetic modification of farm animals provides a means of studying genes involved in a variety of biological systems and disease processes. Moreover, genetically modified farm animals have created a new form of 'pharming' whereby farm animals serve as bioreactors for production of pharmaceuticals or organ donors. A major limitation of cloning procedures is the extreme inefficiency for producing live offspring. Dolly was the only live offspring produced after 277 attempts. Similar inefficiencies for cloning adult animals of other species have been described by others. Many factors related to cloning procedures and culture environment contribute to the death of clones, both in the embryonic and fetal periods as well as during neonatal life. Extreme inefficiencies of this magnitude, along with the fact that death of the surrogate may occur, continue to raise great concerns with cloning humans.

  2. Telomere Elongation and Naive Pluripotent Stem Cells Achieved from Telomerase Haplo-Insufficient Cells by Somatic Cell Nuclear Transfer

    Directory of Open Access Journals (Sweden)

    Li-Ying Sung

    2014-12-01

    Full Text Available Haplo-insufficiency of telomerase genes in humans leads to telomere syndromes such as dyskeratosis congenital and idiopathic pulmonary fibrosis. Generation of pluripotent stem cells from telomerase haplo-insufficient donor cells would provide unique opportunities toward the realization of patient-specific stem cell therapies. Recently, pluripotent human embryonic stem cells (ntESCs have been efficiently achieved by somatic cell nuclear transfer (SCNT. We tested the hypothesis that SCNT could effectively elongate shortening telomeres of telomerase haplo-insufficient cells in the ntESCs with relevant mouse models. Indeed, telomeres of telomerase haplo-insufficient (Terc+/− mouse cells are elongated in ntESCs. Moreover, ntESCs derived from Terc+/− cells exhibit naive pluripotency as evidenced by generation of Terc+/− ntESC clone pups by tetraploid embryo complementation, the most stringent test of naive pluripotency. These data suggest that SCNT could offer a powerful tool to reprogram telomeres and to discover the factors for robust restoration of telomeres and pluripotency of telomerase haplo-insufficient somatic cells.

  3. Stochastic anomaly of methylome but persistent SRY hypermethylation in disorder of sex development in canine somatic cell nuclear transfer

    Science.gov (United States)

    Jeong, Young-Hee; Lu, Hanlin; Park, Chi-Hun; Li, Meiyan; Luo, Huijuan; Kim, Joung Joo; Liu, Siyang; Ko, Kyeong Hee; Huang, Shujia; Hwang, In Sung; Kang, Mi Na; Gong, Desheng; Park, Kang Bae; Choi, Eun Ji; Park, Jung Hyun; Jeong, Yeon Woo; Moon, Changjong; Hyun, Sang-Hwan; Kim, Nam Hyung; Jeung, Eui-Bae; Yang, Huanming; Hwang, Woo Suk; Gao, Fei

    2016-01-01

    Somatic cell nuclear transfer (SCNT) provides an excellent model for studying epigenomic reprogramming during mammalian development. We mapped the whole genome and whole methylome for potential anomalies of mutations or epimutations in SCNT-generated dogs with XY chromosomal sex but complete gonadal dysgenesis, which is classified as 78, XY disorder of sex development (DSD). Whole genome sequencing revealed no potential genomic variations that could explain the pathogenesis of DSD. However, extensive but stochastic anomalies of genome-wide DNA methylation were discovered in these SCNT DSD dogs. Persistent abnormal hypermethylation of the SRY gene was observed together with its down-regulated mRNA and protein expression. Failure of SRY expression due to hypermethylation was further correlated with silencing of a serial of testis determining genes, including SOX9, SF1, SOX8, AMH and DMRT1 in an early embryonic development stage at E34 in the XYDSD gonad, and high activation of the female specific genes, including FOXL2, RSPO1, CYP19A1, WNT4, ERα and ERβ, after one postnatal year in the ovotestis. Our results demonstrate that incomplete demethylation on the SRY gene is the driving cause of XYDSD in these XY DSD dogs, indicating a central role of epigenetic regulation in sex determination. PMID:27501986

  4. Generation of porcine fetal fibroblasts expressing the tetracycline-inducible Cas9 gene by somatic cell nuclear transfer

    Science.gov (United States)

    Liu, Guoqian; Liu, Kai; Wei, Hengxi; Li, Li; Zhang, Shouquan

    2016-01-01

    Cas9 endonuclease, from so-called clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated (Cas) systems of Streptococcus pyogenes, type II functions as an RNA-guided endonuclease and edits the genomes of prokaryotic and eukaryotic organisms, including deletion and insertion by DNA double-stranded break repair mechanisms. In previous studies, it was observed that Cas9, with a genome-scale lentiviral single-guide RNA library, could be applied to a loss-of-function genetic screen, although the loss-of-function genes have yet to be verified in vitro and this approach has not been used in porcine cells. Based on these observations, lentiviral Cas9 was used to infect porcine primary fibroblasts to achieve cell colonies carrying Cas9 endonuclease. Subsequently, porcine fetal fibroblasts expressing the tetracycline-inducible Cas9 gene were generated by somatic cell nuclear transfer, and three 30 day transgenic porcine fetal fibroblasts (PFFs) were obtained. Polymerase chain reaction (PCR), reverse transcription-PCR and western blot analysis indicated that the PFFs were Cas9-positive. In addition, one of the three integrations was located near to known functional genes in the PFF1 cell line, whereas neither of the integrations was located in the PFF1 or PFF2 cell lines. It was hypothesized that these transgenic PFFs may be useful for conditional genomic editing in pigs, and for generating ideal modified porcine models. PMID:27430306

  5. Stochastic anomaly of methylome but persistent SRY hypermethylation in disorder of sex development in canine somatic cell nuclear transfer.

    Science.gov (United States)

    Jeong, Young-Hee; Lu, Hanlin; Park, Chi-Hun; Li, Meiyan; Luo, Huijuan; Kim, Joung Joo; Liu, Siyang; Ko, Kyeong Hee; Huang, Shujia; Hwang, In Sung; Kang, Mi Na; Gong, Desheng; Park, Kang Bae; Choi, Eun Ji; Park, Jung Hyun; Jeong, Yeon Woo; Moon, Changjong; Hyun, Sang-Hwan; Kim, Nam Hyung; Jeung, Eui-Bae; Yang, Huanming; Hwang, Woo Suk; Gao, Fei

    2016-01-01

    Somatic cell nuclear transfer (SCNT) provides an excellent model for studying epigenomic reprogramming during mammalian development. We mapped the whole genome and whole methylome for potential anomalies of mutations or epimutations in SCNT-generated dogs with XY chromosomal sex but complete gonadal dysgenesis, which is classified as 78, XY disorder of sex development (DSD). Whole genome sequencing revealed no potential genomic variations that could explain the pathogenesis of DSD. However, extensive but stochastic anomalies of genome-wide DNA methylation were discovered in these SCNT DSD dogs. Persistent abnormal hypermethylation of the SRY gene was observed together with its down-regulated mRNA and protein expression. Failure of SRY expression due to hypermethylation was further correlated with silencing of a serial of testis determining genes, including SOX9, SF1, SOX8, AMH and DMRT1 in an early embryonic development stage at E34 in the XY(DSD) gonad, and high activation of the female specific genes, including FOXL2, RSPO1, CYP19A1, WNT4, ERα and ERβ, after one postnatal year in the ovotestis. Our results demonstrate that incomplete demethylation on the SRY gene is the driving cause of XY(DSD) in these XY DSD dogs, indicating a central role of epigenetic regulation in sex determination. PMID:27501986

  6. 3D modeling of heat transfer and gas flow in a grooved ring fuel element for nuclear thermal propulsion

    Science.gov (United States)

    Barkett, Laura Ashley

    In the past, fuel elements with multiple axial coolant channels have been used in nuclear propulsion applications. A novel fuel element concept that reduces weight and increases efficiency uses a stack of grooved rings. Each fuel ring consists of a hole on the interior and grooves across the top face. Many grooved ring configurations have been modeled, and a single flow channel for each design has been analyzed. For increased efficiency, a fuel ring with a higher surface-area-to-volume ratio is ideal. When grooves are shallower and they have a lower surface area, the results show that the exit temperature is higher. By coupling the physics of fluid flow with those of heat transfer, the effects on the cooler gas flowing through the grooves of the hot, fissioning ring can be predicted. Models also show differences in velocities and temperatures after dense boundary nodes are applied. Parametric studies were done to show how a pressure drop across the length of the channels will affect the exit temperatures of the gas. Geometric optimization was done to show the temperature distributions and pressure drops that result from the manipulation of various parameters, and the effects of model scaling was also investigated. The inverse Graetz numbers are plotted against Nusselt numbers, and the results of these values suggest that the gas quickly becomes fully developed, laminar flow, rather than constant turbulent conditions.

  7. Generation of PPARγ mono-allelic knockout pigs via zinc-finger nucleases and nuclear transfer cloning

    Institute of Scientific and Technical Information of China (English)

    Dongshan Yang; Jiangtian Tian; Feng Li; Jifeng Zhang; Lin Chang; Duanqing Pei; Y Eugene Chen; Liangxue Lai; Huaqiang Yang; Wei Li; Bentian Zhao; Zhen Ouyang; Zhaoming Liu; Yu Zhao; Nana Fan; Jun Song

    2011-01-01

    @@ Dear Editor, Gene targeting in mouse embryonic stem (ES) cells has revolutionized the field of mouse genetics and allowed for the analysis of diverse aspects of gene function in vivo.For more than two decades,researchers have been actively searching for ES cells from large animals such as pigs and cattle.Unfortunately,to date,no ES cell lines from large animals have passed the crucial test of germ line contribution.The sole method of gene targeting to date in these species remains somatic cell nuclear transfer (SCNT) combined with DNA homologous recombination (HR).Due to the limited proliferation competency and extremely low frequency of HR in somatic cells (less than 10-6),this process is highly inefficient and only a few successful examples have been achieved,even though enrichment strategies such as positivenegative marker selection,promoter-trap and adenoassociated viral delivery were previously used [1-3].The low efficiency of gene targeting in cultured somatic cells is the main barrier for gene targeting in large animals.Recently,zinc-finger nuclease (ZFN) technology has emerged as a powerful tool for genome editing.The success of ZFN technology for gene targeting in fruit flies,zebra fish,rodents as well as human cell lines encouraged us to establish a high-efficiency gene-targeting platform in large animals such as pigs [4-8].

  8. Morin suppresses inflammatory cytokine expression by downregulation of nuclear factor-κB and mitogen-activated protein kinase (MAPK) signaling pathways in lipopolysaccharide-stimulated primary bovine mammary epithelial cells.

    Science.gov (United States)

    Wang, Jingjing; Guo, Changming; Wei, Zhengkai; He, Xuexiu; Kou, Jinhua; Zhou, Ershun; Yang, Zhengtao; Fu, Yunhe

    2016-04-01

    Morin, a flavonoid isolated from Chinese herbs of the Moraceae family, has been reported to possess antiinflammatory activity. However, the effects of morin on mastitis have not been investigated. The present study was conducted to elucidate the antiinflammatory properties of morin on lipopolysaccharide (LPS)-stimulated primary bovine mammary epithelial cells (bMEC). The viability of bMEC was analyzed by MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium] assay. Subsequently, bMEC were stimulated with LPS in the presence or absence of morin. Gene expression of proinflammatory cytokines was determined by quantitative real-time PCR (qRT-PCR). Nuclear factor-κB (NF-κB), inhibitory kappa B (IκBα) protein, extracellular signal-regulated kinase (ERK), p38, and c-Jun N-terminal kinase (JNK) were detected by Western blotting. The results showed that cell viability was not affected by morin. Moreover, morin inhibited the gene expression of tumor necrosis factor-α (TNF-α), IL-6, and IL-1β in LPS-stimulated bMEC in a dose-dependent manner. Western blot analysis showed that morin suppressed the phosphorylation of IκBα, NF-κB unit p65, ERK, p38, and JNK in LPS-stimulated bMEC. In conclusion, the protective effects of morin on LPS-induced inflammatory response in bMEC may be due to its ability to suppress NF-κB and mitogen-activated protein kinase (MAPK) signaling pathways. These findings suggest that morin may be used as antiinflammatory drug for mastitis.

  9. Communications received from certain Member States regarding guidelines for transfers of nuclear-related dual-use equipment, materials, software and related technology

    International Nuclear Information System (INIS)

    The Director General of the International Atomic Energy Agency has received Notes Verbales dated 28 February 2003 from the Resident Representatives to the Agency of Argentina, Austria, Belgium, Bulgaria, Canada, Cyprus, Czech Republic, Denmark, Finland, France, Germany, Greece, Hungary, Italy, Japan, Kazakhstan, Latvia, Netherlands, Norway, Portugal, Republic of Korea, Slovakia, Slovenia, South Africa, Spain, Sweden, Switzerland, Turkey, Ukraine, the United Kingdom of Great Britain and Northern Ireland, and the United States of America, relating to transfers of nuclear-related dual-use equipment, materials, software and related technology. The purpose of the Note Verbales is to provide further information on those Governments' Guidelines for Transfers of Nuclear-related Dual-use Equipment, Materials, Software and Related Technology. In the light of the wish expressed at the end of each Note Verbale, the text of the Notes Verbales is attached. The attachment to these Notes Verbales is also reproduced in full

  10. Communications received from certain Member States regarding guidelines for transfers of nuclear-related dual-use equipment, materials, software and related technology

    International Nuclear Information System (INIS)

    The Director General of the International Atomic Energy Agency has received Notes Verbales dated 25 October 2004 from the Resident Representatives to the Agency of Argentina, Australia, Austria, Belgium, Brazil, Canada, Cyprus, Czech Republic, Estonia, Finland, France, Greece, Hungary, Italy, Japan, Malta, Netherlands, Norway, Republic of Korea, South Africa, Spain, Sweden, Turkey, Ukraine, the United Kingdom of Great Britain and Northern Ireland and the United States of America, relating to transfers of nuclear-related dual-use equipment, materials, software and related technology. The purpose of the Notes Verbales is to provide further information on those Governments' guidelines for transfers of nuclear-related dual-use equipment, materials, software and related technology. In the light of the wish expressed at the end of each Note Verbale, the text of the Notes Verbales is attached. The attachment to these Notes Verbales is also reproduced in full

  11. Cartilage (Bovine and Shark) (PDQ)

    Science.gov (United States)

    ... Ask about Your Treatment Research Cartilage (Bovine and Shark) (PDQ®)–Patient Version Overview Go to Health Professional ... 8 ). Questions and Answers About Cartilage (Bovine and Shark) What is cartilage? Cartilage is a type of ...

  12. Structure and Function of Bovine and Camel Chymosin

    DEFF Research Database (Denmark)

    Jensen, Jesper Langholm

    The central step in cheese making is the separation of milk into curd and whey. This can be done enzymatically by hydrolysis of the Phe105-Met106 bond or nearby bonds in bovine κ-casein, which releases its hydrophilic C-terminal leading to coagulation of the milk. The preferred enzyme...... and characterised, and turned out to have an even higher activity and specificity towards the Phe105-Met106 bond than bovine chymosin. The sequences of bovine and camel chymosin are 85% identical, and yet they have significantly different cheese making properties. The aim of the project was to explain...... this difference through the study of the structures of bovine and camel chymosin, and preparation of catalytically inactive enzymes in complex with substrate. Their milk-clotting activities was determined using the traditional assay on skimmed milk, and a fluorescence resonance energy transfer (FRET) assay...

  13. Gene expression, oocyte nuclear maturation and developmental competence of bovine oocytes and embryos produced after in vivo and in vitro heat shock.

    Science.gov (United States)

    Pavani, Krishna C; Baron, Erica; Correia, Pedro; Lourenço, Joana; Bettencourt, Bruno Filipe; Sousa, Madalena; da Silva, Fernando Moreira

    2016-10-01

    Three assays were performed. In assay 1, oocytes harvested during the winter months were subjected to kinetic heat shock by stressing the oocytes at 39.5°C (HS1) or at 40.5°C (HS2) for either 6, 12, 18 or 24 h and then matured at control temperature (38.5°C). The nuclear maturation rates (NMR) of all oocytes were recorded after 24 h. In assay 2, oocytes collected year-round maturated, were implanted via in vitro fertilization (IVF) and developed for 9 days. Gene expression analysis was performed on target genes (Cx43, CDH1, DNMT1, HSPA14) with reference to the two housekeeping genes (GAPDH and SDHA) in embryos. Similarly, in assay 3, genetic analysis was performed on the embryos produced from heat-stressed oocytes (from HS1 and HS2). In assay 1, the duration of heat stress resulted in a significant decline in NMR (P CDH1 genes (P < 0.05). Targeted gene expression was aberrant in embryo development, which can provide evidence on early embryo arrest and slowed embryo development.

  14. A highly efficient method for generation of therapeutic quality human pluripotent stem cells by using naive induced pluripotent stem cells nucleus for nuclear transfer

    OpenAIRE

    Sanal, Madhusudana Girija

    2014-01-01

    Even after several years since the discovery of human embryonic stem cells and induced pluripotent stem cells (iPSC), we are still unable to make any significant therapeutic benefits out of them such as cell therapy or generation of organs for transplantation. Recent success in somatic cell nuclear transfer (SCNT) made it possible to generate diploid embryonic stem cells, which opens up the way to make high-quality pluripotent stem cells. However, the process is highly inefficient and hence e...

  15. Effects of nuclear deformation and neutron transfer in capture process, and origin of fusion hindrance at deep sub-barrier energies

    OpenAIRE

    Sargsyan, V. V.; Adamian, G.G.; Antonenko, N.V.; W. Scheid; Zhang, H. Q.

    2011-01-01

    The roles of nuclear deformation and neutron transfer in sub-barrier capture process are studied within the quantum diffusion approach. The change of the deformations of colliding nuclei with neutron exchange can crucially influence the sub-barrier fusion. The comparison of the calculated capture cross section and the measured fusion cross section in various reactions at extreme sub- barrier energies gives us information about the fusion and quasifission.

  16. Absence of nucleolus formation in raccoon dog-porcine interspecies somatic cell nuclear transfer embryos results in embryonic developmental failure.

    Science.gov (United States)

    Jeon, Yubyeol; Nam, Yeong-Hee; Cheong, Seung-A; Kwak, Seong-Sung; Lee, Eunsong; Hyun, Sang-Hwan

    2016-08-25

    Interspecies somatic cell nuclear transfer (iSCNT) can be a solution for preservation of endangered species that have limited oocytes. It has been reported that blastocyst production by iSCNT is successful even if the genetic distances between donors and recipients are large. In particular, domestic pig oocytes can support the development of canine to porcine iSCNT embryos. Therefore, we examined whether porcine oocytes may be suitable recipient oocytes for Korean raccoon dog iSCNT. We investigated the effects of trichostatin A (TSA) treatment on iSCNT embryo developmental patterns and nucleolus formation. Enucleated porcine oocytes were fused with raccoon dog fibroblasts by electrofusion and cleavage, and blastocyst development and nucleolus formation were evaluated. To our knowledge, this study is the first in which raccoon dog iSCNT was performed using porcine oocytes; we found that 68.5% of 158 iSCNT embryos had the ability to cleave. However, these iSCNT embryos did not develop past the 4-cell stage. Treatment with TSA did not affect iSCNT embryonic development; moreover, the nuclei failed to form nucleoli at 48 and 72 h post-activation (hpa). In contrast, pig SCNT embryos of the control group showed 18.8% and 87.9% nucleolus formation at 48 and 72 hpa, respectively. Our results demonstrated that porcine cytoplasts efficiently supported the development of raccoon dog iSCNT embryos to the 4-cell stage, the stage of porcine embryonic genome activation (EGA); however, these embryos failed to reach the blastocyst stage and showed defects in nucleolus formation.

  17. Comparison of the efficiency of Banna miniature inbred pig somatic cell nuclear transfer among different donor cells.

    Directory of Open Access Journals (Sweden)

    Hongjiang Wei

    Full Text Available Somatic cell nuclear transfer (SCNT is an important method of breeding quality varieties, expanding groups, and preserving endangered species. However, the viability of SCNT embryos is poor, and the cloned rate of animal production is low in pig. This study aims to investigate the gene function and establish a disease model of Banna miniature inbred pig. SCNT with donor cells derived from fetal, newborn, and adult fibroblasts was performed, and the cloning efficiencies among the donor cells were compared. The results showed that the cleavage and blastocyst formation rates did not significantly differ between the reconstructed embryos derived from the fetal (74.3% and 27.4% and newborn (76.4% and 21.8% fibroblasts of the Banna miniature inbred pig (P>0.05. However, both fetal and newborn fibroblast groups showed significantly higher rates than the adult fibroblast group (61.9% and 13.0%; P<0.05. The pregnancy rates of the recipients in the fetal and newborn fibroblast groups (60% and 80%, respectively were higher than those in the adult fibroblast group. Eight, three, and one cloned piglet were obtained from reconstructed embryos of the fetal, newborn, and adult fibroblasts, respectively. Microsatellite analyses results indicated that the genotypes of all cloning piglets were identical to their donor cells and that the genetic homozygosity of the Banna miniature inbred pig was higher than those of the recipients. Therefore, the offspring was successfully cloned using the fetal, newborn, and adult fibroblasts of Banna miniature inbred pig as donor cells.

  18. Comparison of the results of several heat transfer computer codes when applied to a hypothetical nuclear waste repository

    International Nuclear Information System (INIS)

    A direct comparison of transient thermal calculations was made with the heat transfer codes HEATING5, THAC-SIP-3D, ADINAT, SINDA, TRUMP, and TRANCO for a hypothetical nuclear waste repository. With the exception of TRUMP and SINDA (actually closer to the earlier CINDA3G version), the other codes agreed to within +-5% for the temperature rises as a function of time. The TRUMP results agreed within +-5% up to about 50 years, where the maximum temperature occurs, and then began an oscillary behavior with up to 25% deviations at longer times. This could have resulted from time steps that were too large or from some unknown system problems. The available version of the SINDA code was not compatible with the IBM compiler without using an alternative method for handling a variable thermal conductivity. The results were about 40% low, but a reasonable agreement was obtained by assuming a uniform thermal conductivity; however, a programming error was later discovered in the alternative method. Some work is required on the IBM version to make it compatible with the system and still use the recommended method of handling variable thermal conductivity. TRANCO can only be run as a 2-D model, and TRUMP and CINDA apparently required longer running times and did not agree in the 2-D case; therefore, only HEATING5, THAC-SIP-3D, and ADINAT were used for the 3-D model calculations. The codes agreed within +-5%; at distances of about 1 ft from the waste canister edge, temperature rises were also close to that predicted by the 3-D model

  19. Using a nano-flare probe to detect RNA in live donor cells prior to somatic cell nuclear transfer.

    Science.gov (United States)

    Fu, Bo; Ren, Liang; Liu, Di; Ma, Jian-Zhang; An, Tie-Zhu; Yang, Xiu-Qin; Ma, Hong; Guo, Zhen-Hua; Zhu, Meng; Bai, Jing

    2016-01-01

    Many transgenes are silenced in mammalian cells (donor cells used for somatic cell nuclear transfer [SCNT]). Silencing correlated with a repressed chromatin structure or suppressed promoter, and it impeded the production of transgenic animals. Gene transcription studies in live cells are challenging because of the drawbacks of reverse-transcription polymerase chain reaction and fluorescence in situ hybridization. Nano-flare probes provide an effective approach to detect RNA in living cells. We used 18S RNA, a housekeeping gene, as a reference gene. This study aimed to establish a platform to detect RNA in single living donor cells using a Nano-flare probe prior to SCNT and to verify the safety and validity of the Nano-flare probe in order to provide a technical foundation for rescuing silenced transgenes in transgenic cloned embryos. We investigated cytotoxic effect of the 18S RNA-Nano-flare probe on porcine fetal fibroblasts, characterized the distribution of the 18S RNA-Nano-flare probe in living cells and investigated the effect of the 18S RNA-Nano-flare probe on the development of cloned embryos after SCNT. The cytotoxic effect of the 18S RNA-Nano-flare probe on porcine fetal fibroblasts was dose-dependent, and 18S RNA was detected using the 18S RNA-Nano-flare probe. In addition, treating donor cells with 500 pM 18S RNA-Nano-flare probe did not have adverse effects on the development of SCNT embryos at the pre-implantation stage. In conclusion, we established a preliminary platform to detect RNA in live donor cells using a Nano-flare probe prior to SCNT.

  20. Radioecological transfer of {sup 137}Cs from ground deposition to man from Chernobyl debris and from nuclear weapons fallout in different Swedish populations

    Energy Technology Data Exchange (ETDEWEB)

    Raeaef, C.L. [Malmoe Univ. Hospital, Lund Univ., Dept. of Radiation Physics, Malmoe (Sweden)

    2005-07-01

    A comparison of the estimated committed effective dose per unit activity deposition on ground was made between different critical groups in Sweden. The time-integrated aggregate transfer of {sup 137}Cs for the global fallout was 2-3 times higher than from Chernobyl debris for Swedish urban populations. For reindeer herders this difference is even more marked, with a factor of three to four higher time-integrated transfer factor of nuclear weapons fallout. Considering the transfer of Chernobyl {sup 137}Cs debris the time-integrated transfer factor appears to be more than 25 times higher for reindeer herders in Sweden than for the urban reference groups. An even more pronounced relative difference between the time integrated aggregate transfer was observed between reindeer herders and urban reference populations for the pre-Chernobyl fallout (a factor of 30). The projected committed effective dose from internal contamination of Chernobyl {sup 137}Cs per unit activity deposition is observed to be 2030 {mu}Sv/kBq m{sup -2}. The highest values in Sweden are obtained for reindeer herders with an estimated radioecological transfer of 0.5 mSv/kBq m{sup -2}. (au)

  1. An Overview of strategic measures to assess workforce needs and ensure technology transfer to meet current and future nuclear power operations

    International Nuclear Information System (INIS)

    Between 1956 and 1989, the number of operating commercial nuclear power plants in the United States increased from none to 109. With the exception of a few plants that were still in final construction, no new nuclear power plants were ordered in the United States as the new millennium began. In 2005, the federal government pronounced the need for new electric power generating systems during the first quarter of the 21. century. The need comes from a desire to curb our reliance on fossil fuels, as well as to provide for a cleaner environment. One of those fuel systems noted was nuclear energy. Given the time between the last active period of nuclear power plant development and construction, there is a need to supply a talented and well-prepared workforce to operate the new plants. It will also be necessary to assess the needs of our current fleet of operating nuclear power plants, of which many are in the process of re-licensing, yet also facing an aging plant workforce. This paper will review and discuss measures to assess diverse workforce needs and technology transfer to meet current licensing requirements as that of future nuclear power plant development in the United States. (authors)

  2. Replication of somatic micronuclei in bovine enucleated oocytes

    Directory of Open Access Journals (Sweden)

    Canel Natalia

    2012-11-01

    Full Text Available Abstract Background Microcell-mediated chromosome transfer (MMCT was developed to introduce a low number of chromosomes into a host cell. We have designed a novel technique combining part of MMCT with somatic cell nuclear transfer, which consists of injecting a somatic micronucleus into an enucleated oocyte, and inducing its cellular machinery to replicate such micronucleus. It would allow the isolation and manipulation of a single or a low number of somatic chromosomes. Methods Micronuclei from adult bovine fibroblasts were produced by incubation in 0.05 μg/ml demecolcine for 46 h followed by 2 mg/ml mitomycin for 2 h. Cells were finally treated with 10 μg/ml cytochalasin B for 1 h. In vitro matured bovine oocytes were mechanically enucleated and intracytoplasmatically injected with one somatic micronucleus, which had been previously exposed [Micronucleus- injected (+] or not [Micronucleus- injected (−] to a transgene (50 ng/μl pCX-EGFP during 5 min. Enucleated oocytes [Enucleated (+] and parthenogenetic [Parthenogenetic (+] controls were injected into the cytoplasm with less than 10 pl of PVP containing 50 ng/μl pCX-EGFP. A non-injected parthenogenetic control [Parthenogenetic (−] was also included. Two hours after injection, oocytes and reconstituted embryos were activated by incubation in 5 μM ionomycin for 4 min + 1.9 mM 6-DMAP for 3 h. Cleavage stage and egfp expression were evaluated. DNA replication was confirmed by DAPI staining. On day 2, Micronucleus- injected (−, Parthenogenetic (− and in vitro fertilized (IVF embryos were karyotyped. Differences among treatments were determined by Fisher′s exact test (p≤0.05. Results All the experimental groups underwent the first cell divisions. Interestingly, a low number of Micronucleus-injected embryos showed egfp expression. DAPI staining confirmed replication of micronuclei in most of the evaluated embryos. Karyotype analysis revealed that all Micronucleus-injected embryos had

  3. 77 FR 15847 - Bovine Spongiform Encephalopathy; Importation of Bovines and Bovine Products

    Science.gov (United States)

    2012-03-16

    ..., ``Analysis of Bovine Spongiform Encephalopathy (BSE) Risk to the U.S. Cattle Population from Importation of... final rule did not limit the importation of bovine-derived meat from Canada to that derived from cattle... meat from bovines 30 months of age or older while continuing to prohibit the importation of live...

  4. BOVINE VIRAL DIARRHEA VIRUSES

    Science.gov (United States)

    Bovine viral diarrhea virus (BVDV) is an umbrella term for two species of viruses, BVDV1 and BVDV2, within the Pestivirus genus of the Flavivirus family. BVDV viruses are further subclassified as cytopathic and noncytopathic based on their activity in cultured epithelial cells. Noncytopathic BVDV p...

  5. Bovine Spongiform Encephalopathy

    Science.gov (United States)

    Bovine spongiform encephalopathy (BSE), also referred to as “mad cow disease” is a chronic, non-febrile, neuro-degenerative disease affecting the central nervous system. The transmissible spongiform encephalopathies (TSEs) of domestic animals, of which BSE is a member includes scrapie of sheep...

  6. Bovine milk exosome proteome

    Science.gov (United States)

    Exosomes are 40-100 nm membrane vesicles of endocytic origin and are found in blood, urine, amniotic fluid, bronchoalveolar lavage (BAL) fluid, as well as human and bovine milk. Exosomes are extracellular organelles important in intracellular communication/signaling, immune function, and biomarkers ...

  7. Analysis of Heat Transfer Characteristics Based on Design Factors for Determining the Internal Geometry of Metal Insulation in Nuclear Power Plant

    Energy Technology Data Exchange (ETDEWEB)

    Song, Ki O; Yu, Jeong Ho; Lee, Tae Ho; Jeon, Hyun Ik; Ha, Seung Woo; Cho, Sun Young [KLES Inc., Seoul (Korea, Republic of)

    2015-11-15

    A heat insulating material used in the industrial site normally derives its heat insulating performance by using a low thermal conductivity material such as glass fiber. In case of the metal insulation for nuclear power plant, in contrast, only TP 304 stainless steel foil having high thermal conductivity is the only acceptable material. So, it is required to approach in structural aspect to ensure the insulation performance. In this study, the design factors related to the metal insulation internal structure were determined considering the three modes of heat transfer, i.e., conduction, convection, and radiation. The analysis of heat flow was used to understand the ratio of the heat transfer from each factor to the overall heat transfer from all the factors. Based on this study, in order to minimize the convection phenomenon caused by the internal insulation, a multiple foil was inserted in the insulation. The increase in the conduction heat transfer rate was compared, and the insulation performance under the three modes of heat transfer was analyzed in order to determine the internal geometry.

  8. Analysis of heat transfer characteristics based on design factors for determining the internal geometry of metal insulation in nuclear power plant

    Energy Technology Data Exchange (ETDEWEB)

    Song, Ki O; Yu, Jeong Ho; Lee, Tae Ho; Jeon, Hyun Ik; Ha, Seung Woo; Cho, Sun Young [KLES Inc., Daejeon (Korea, Republic of)

    2015-11-15

    A heat insulating material used in the industrial site normally derives its heat insulating performance by using a low thermal conductivity material such as glass fiber. In case of the metal insulation for nuclear power plant, in contrast, only TP 304 stainless steel foil having high thermal conductivity is the only acceptable material. So, it is required to approach in structural aspect to ensure the insulation performance. In this study, the design factors related to the metal insulation internal structure were determined considering the three modes of heat transfer, i.e., conduction, convection, and radiation. The analysis of heat flow was used to understand the ratio of the heat transfer from each factor to the overall heat transfer from all the factors. Based on this study, in order to minimize the convection phenomenon caused by the internal insulation, a multiple foil was inserted in the insulation. The increase in the conduction heat transfer rate was compared, and the insulation performance under the three modes of heat transfer was analyzed in order to determine the internal geometry.

  9. 小檗碱与牛血清白蛋白相互作用的核磁共振研究%Nuclear Magnetic Resonance Studies on Interactions of Berberine and Bovine Serum Albumin

    Institute of Scientific and Technical Information of China (English)

    吴丽敏; 张美玲; 娄依依; 李旸; 赵良才; 黄可新; 高红昌

    2011-01-01

    采用核磁共振波谱技术(NMR)研究小檗碱与牛血清白蛋白(BSA)的相互结合作用.比较不同浓度小檗碱在一定浓度的BSA溶液中的化学位移、弛豫时间和扩散系数的变化情况.研究表明,小檗碱环上芳香质子与BSA的结合作用较强,其它烷基质子与BSA结合作用较弱;BSA的结合使得小檗碱各个质子的1HT1弛豫时间明显减少,质子在溶液中运动受限;小檗碱与BSA相互作用的饱和浓度之比为60:1;根据自扩散系数拟合可以得到两者解离常数为0.44 mmol/L;结合位点数为22.本研究为临床安全、合理使用小檗碱,以及开发改良小檗碱类似药物提供实验依据.%1H nuclear magnetic resonance chemical shift, relaxation time and diffusion coefficient measurements were carried out to study the interaction on the affinity binding of Berberine, an anti-bacterial drug, to bovine serum albumin (BSA). The results showed that the protons of aromatic ring of Berberine were more favorable to form Berberine-BSA complex than those of aliphatic groups. BSA combination resulted in significantly decrease in the relaxation time of Berberine protons, which sug-gests that the motion of these protons were limited in aqueous solutions. The saturation concentration ratio of Berberine to BSA is 60:1 according to the measurements of chemical shift and relaxation time. The apparent dissociation constant was 0. 44 mmol/L and the binding sites of Berberine molecules on BSA was 22 fitted from the self-diffusion measurements. This study is of great importance for the safe and rational use of Berberine in clinic, and development of improved Berberine analogs.

  10. Conjugation of Chitooligosaccharide-5-fluorouracil with Bovine Serum Albumin

    Institute of Scientific and Technical Information of China (English)

    Rong Min WANG; Jing Feng SONG; Yu Feng HE; Juan Juan MAO; Yan LI

    2006-01-01

    The interaction between chitooligosaccharide-5-fluorouracil (COS-5FU) and bovine serum albumin (BSA) was studied by fluorescence spectroscopy. It was found that an energy transfer between COS-5FU and BSA had been occurred. The binding constants were calculated,between the donor and acceptor, the distance between BSA and COS-5FU was determined.

  11. Binding interactions of pefloxacin mesylate with bovine lactoferrin and human serum albumin

    Institute of Scientific and Technical Information of China (English)

    FAN Ji-cai; CHEN Xiang; WANG Yun; FAN Cheng-ping; SHANG Zhi-cai

    2006-01-01

    The binding of pefloxacin mesylate (PFLX) to bovine lactoferrin (BLf) and human serum albumin (HSA) in dilute aqueous solution was studied using fluorescence spectra and absorbance spectra. The binding constant K and the binding sites n were obtained by fluorescence quenching method. The binding distance r and energy-transfer efficiency E between pefloxacin mesylate and bovine lactoferrin as well as human serum albumin were also obtained according to the mechanism of Forster-type dipole-dipole nonradiative energy-transfer. The effects of pefloxacin mesylate on the conformations of bovine lactoferrin and human serum albumin were also analyzed using synchronous fluorescence spectroscopy.

  12. Genome-wide epigenetic alterations in cloned bovine fetuses.

    Science.gov (United States)

    Cezar, Gabriela Gebrin; Bartolomei, Marisa S; Forsberg, Erik J; First, Neal L; Bishop, Michael D; Eilertsen, Kenneth J

    2003-03-01

    To gain a better understanding of global methylation differences associated with development of nuclear transfer (NT)-generated cattle, we analyzed the genome-wide methylation status of spontaneously aborted cloned fetuses, cloned fetuses, and adult clones that were derived from transgenic and nontransgenic cumulus, genital ridge, and body cell lines. Cloned fetuses were recovered from ongoing normal pregnancies and were morphologically normal. Fetuses generated by artificial insemination (AI) were used as controls. In vitro fertilization (IVF) fetuses were compared with AI controls to assess effects of in vitro culture on the 5-methylcytosine content of fetal genomes. All of the fetuses were female. Skin biopsies were obtained from cloned and AI-generated adult cows. All of the adult clones were phenotypically normal and lactating and had no history of health or reproductive disorders. Genome-wide cytosine methylation levels were monitored by reverse-phase HPLC, and results indicated reduced levels of methylated cytosine in NT-generated fetuses. In contrast, no differences were observed between adult, lactating clones and similarly aged lactating cows produced by AI. These data imply that survivability of cloned cattle may be closely related to the global DNA methylation status. This is the first report to indicate that global methylation losses may contribute to the developmental failure of cloned bovine fetuses. PMID:12604655

  13. The transfer of the nuclear supervision into the federal self-administration in the view of the constitutional law; Die Ueberfuehrung der Atomaufsicht in die Bundeseigenverwaltung aus verfassungsrechtlicher Sicht

    Energy Technology Data Exchange (ETDEWEB)

    Burgi, M. [Bochum Univ. (Germany)

    2005-07-01

    The paper is focussed on the question of a possible transfer of the nuclear supervision from the federal executive administration into a federal self-administration. The discussed topics include the characterization of the nuclear supervision tasks, the relation between administrative tasks and the type of administration, an assessment of the precondition of centrality with respect to the nuclear supervision and a possible accomplishment of the so called centrality.

  14. Effect of nuclear factor κB inhibition on serotype 9 adeno-associated viral (AAV9) minidystrophin gene transfer to the mdx mouse.

    Science.gov (United States)

    Reay, Daniel P; Niizawa, Gabriela A; Watchko, Jon F; Daood, Molly; Reay, Ja'Nean C; Raggi, Eugene; Clemens, Paula R

    2012-01-01

    Gene therapy studies for Duchenne muscular dystrophy (DMD) have focused on viral vector-mediated gene transfer to provide therapeutic protein expression or treatment with drugs to limit dystrophic changes in muscle. The pathological activation of the nuclear factor (NF)-κB signaling pathway has emerged as an important cause of dystrophic muscle changes in muscular dystrophy. Furthermore, activation of NF-κB may inhibit gene transfer by promoting inflammation in response to the transgene or vector. Therefore, we hypothesized that inhibition of pathological NF-κB activation in muscle would complement the therapeutic benefits of dystrophin gene transfer in the mdx mouse model of DMD. Systemic gene transfer using serotype 9 adeno-associated viral (AAV9) vectors is promising for treatment of preclinical models of DMD because of vector tropism to cardiac and skeletal muscle. In quadriceps of C57BL/10ScSn-Dmd(mdx)/J (mdx) mice, the addition of octalysine (8K)-NF-κB essential modulator (NEMO)-binding domain (8K-NBD) peptide treatment to AAV9 minidystrophin gene delivery resulted in increased levels of recombinant dystrophin expression suggesting that 8K-NBD treatment promoted an environment in muscle tissue conducive to higher levels of expression. Indices of necrosis and regeneration were diminished with AAV9 gene delivery alone and to a greater degree with the addition of 8K-NBD treatment. In diaphragm muscle, high-level transgene expression was achieved with AAV9 minidystoophin gene delivery alone; therefore, improvements in histological and physiological indices were comparable in the two treatment groups. The data support benefit from 8K-NBD treatment to complement gene transfer therapy for DMD in muscle tissue that receives incomplete levels of transduction by gene transfer, which may be highly significant for clinical applications of muscle gene delivery. PMID:22231732

  15. Identification of short hairpin RNA targeting foot-and-mouth disease virus with transgenic bovine fetal epithelium cells.

    Directory of Open Access Journals (Sweden)

    Hongmei Wang

    Full Text Available BACKGROUND: Although it is known that RNA interference (RNAi targeting viral genes protects experimental animals, such as mice, from the challenge of Foot-and-mouth disease virus (FMDV, it has not been previously investigated whether shRNAs targeting FMDV in transgenic dairy cattle or primary transgenic bovine epithelium cells will confer resistance against FMDV challenge. PRINCIPAL FINDING: Here we constructed three recombinant lentiviral vectors containing shRNA against VP2 (RNAi-VP2, VP3 (RNAi-VP3, or VP4 (RNAi-VP4 of FMDV, and found that all of them strongly suppressed the transient expression of a FLAG-tagged viral gene fusion protein in 293T cells. In BHK-21 cells, RNAi-VP4 was found to be more potent in inhibition of viral replication than the others with over 98% inhibition of viral replication. Therefore, recombinant lentiviral vector RNAi-VP4 was transfected into bovine fetal fibroblast cells to generate transgenic nuclear donor cells. With subsequent somatic cell cloning, we generated forty transgenic blastocysts, and then transferred them to 20 synchronized recipient cows. Three transgenic bovine fetuses were obtained after pregnant period of 4 months, and integration into chromosome in cloned fetuses was confirmed by Southern hybridization. The primary tongue epithelium cells of transgenic fetuses were isolated and inoculated with 100 TCID(50 of FMDV, and it was observed that shRNA significantly suppressed viral RNA synthesis and inhibited over 91% of viral replication after inoculation of FMDV for 48 h. CONCLUSION: RNAi-VP4 targeting viral VP4 gene appears to prevent primary epithelium cells of transgenic bovine fetus from FMDV infection, and it could be a candidate shRNA used for cultivation of transgenic cattle against FMDV.

  16. Nucleologenesis and embryonic genome activation are defective in interspecies cloned embryos between bovine ooplasm and rhesus monkey somatic cells

    Directory of Open Access Journals (Sweden)

    Han Yong-Mahn

    2009-07-01

    Full Text Available Abstract Background Interspecies somatic cell nuclear transfer (iSCNT has been proposed as a tool to address basic developmental questions and to improve the feasibility of cell therapy. However, the low efficiency of iSCNT embryonic development is a crucial problem when compared to in vitro fertilization (IVF and intraspecies SCNT. Thus, we examined the effect of donor cell species on the early development of SCNT embryos after reconstruction with bovine ooplasm. Results No apparent difference in cleavage rate was found among IVF, monkey-bovine (MB-iSCNT, and bovine-bovine (BB-SCNT embryos. However, MB-iSCNT embryos failed to develop beyond the 8- or 16-cell stages and lacked expression of the genes involved in embryonic genome activation (EGA at the 8-cell stage. From ultrastructural observations made during the peri-EGA period using transmission electron microscopy (TEM, we found that the nucleoli of MB-iSCNT embryos were morphologically abnormal or arrested at the primary stage of nucleologenesis. Consistent with the TEM analysis, nucleolar component proteins, such as upstream binding transcription factor, fibrillarin, nucleolin, and nucleophosmin, showed decreased expression and were structurally disorganized in MB-iSCNT embryos compared to IVF and BB-SCNT embryos, as revealed by real-time PCR and immunofluorescence confocal laser scanning microscopy, respectively. Conclusion The down-regulation of housekeeping and imprinting genes, abnormal nucleolar morphology, and aberrant patterns of nucleolar proteins during EGA resulted in developmental failure in MB-iSCNT embryos. These results provide insight into the unresolved problems of early embryonic development in iSCNT embryos.

  17. A study of the role and characteristics of multiple-step transfer mechanisms in quasi-elastic heavy-ion collisions, with emphasis on nuclear spectroscopy

    International Nuclear Information System (INIS)

    Attention is primarily focused on the possible usefulness of heavy-ion induced two-nucleon transfer reactions as a spectroscopic tool, in particular on how nuclear structure, as reflected in transfer form factors, can be probed through shape characteristics of angular distributions. Studies of this type have been gaining increasing interest due to new developments of experimental detection techniques and equipment. This has made measurements of small cross sections (down to l μb) more feasible, and high resolution (40-70 keV) is obtainable with heavy-ion (carbon and oxygen) beams, as was proven by experimental collaborators during the development of the projects on which this thesis is based. The thesis is composed of a selection of publications writting during the period 1973-1976, preceded by an introduction in which the motivation for and the content of the papers in Parts B and C is outlined in condensed form. (JIW)

  18. Communication Received from Certain Member States Regarding Guidelines for Transfers of Nuclear-related Dual-use Equipment, Material, Software and Related Technology

    International Nuclear Information System (INIS)

    The Agency has received a Note Verbale from the Permanent Mission of Hungary, dated 14 June 2010, in which it requests that the Agency circulate to all Member States a letter of 7 May 2010 from the Chairman of the Nuclear Suppliers Group, Ambassador Ms. Gyorgyi Martin Zanathy, to the Director General, on behalf of the Governments of Argentina, Australia, Austria, Belarus, Belgium, Brazil, Bulgaria, Canada, China, Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Japan, Kazakhstan, Republic of Korea, Latvia, Lithuania, Luxemburg, Malta, Netherlands, New Zealand, Norway, Poland, Portugal, Romania, Russian Federation, Slovakia, Slovenia, South Africa, Spain, Sweden, Switzerland, Turkey, Ukraine, the United Kingdom of Great Britain and Northern Ireland and the United States of America,1 providing further information on those Governments’ Guidelines for Nuclear Transfers

  19. Photodynamically generated bovine serum albumin radicals

    DEFF Research Database (Denmark)

    Silvester, J A; Timmins, G S; Davies, Michael Jonathan

    1998-01-01

    Porphyrin-sensitized photoxidation of bovine serum albumin (BSA) results in oxidation of the protein at (at least) two different, specific sites: the Cys-34 residue giving rise to a thiyl radical (RS.); and one or both of the tryptophan residues (Trp-134 and Trp-214) resulting in the formation...... of tertiary carbon-centred radicals and disruption of the tryptophan ring system. In the case of porphyrins such as hematoporphyrin, which bind at specific sites on BSA, these species appear to arise via long-range transfer of damage within the protein structure, as the binding site is some distance from...

  20. Energy metabolism during microsurgical transfer of human skeletal muscle assessed by high-pressure liquid chromatography and by 31P-nuclear magnetic resonance.

    Science.gov (United States)

    Lundberg, Jonas; Elander, Anna; Rakotonirainy, Olivier; Zetterlund, Therese; Fogdestam, Ingemar; Soussi, Bassam

    2002-01-01

    The effect of ischaemia and reperfusion on human skeletal muscle was studied during free vascularised muscle transfer. Muscle biopsy specimens were taken from patients having microsurgical muscle transfer, 18 cases (17 patients; 12 men, 5 women). The biopsies were taken three times: before transfer of the muscle (control), at maximum ischaemic time, and one hour after revascularisation. The biopsy specimens were analysed for purine nucleotides, by high-pressure liquid chromatography (HPLC), and by nuclear magnetic resonance (NMR) at 500 MHz. Phosphocreatine (PCr) recovered only partially (79%) and adenosine triphosphate (ATP) did not differ significantly from normal control after revascularisation and a mean ischaemic time of 114 minutes. NMR measurements showed an accumulation of glucose-6-phosphate (G-6-P) during the ischaemic period, indicating anaerobic metabolism. After three hours of ischaemia and one hour of reperfusion the PCr recovery was less than 60% (r = 0.7). The results confirm those of previous animal studies, which set three hours normothermic ischaemia as a safe limit for tissue preservation when transferring skeletal muscle. Longer ischaemic times may cause serious postoperative healing problems and reduced muscle function.

  1. Assessment of the TiO{sub 2}/water nanofluid effects on heat transfer characteristics in VVER-1000 nuclear reactor using CFD modeling

    Energy Technology Data Exchange (ETDEWEB)

    Mousavizadeh, Seyed Mohammad; Ansarifar, Gholam Reza; Talebi, Mansour [Dept. of Nuclear Engineering, Faculty of Advanced Sciences and Technology, University of Isfahan, Isfahan (Iran, Islamic Republic of)

    2015-12-15

    The most important advantage of nanoparticles is the increased thermal conductivity coefficient and convection heat transfer coefficient so that, as a result of using a 1.5% volume concentration of nanoparticles, the thermal conductivity coefficient would increase by about twice. In this paper, the effects of a nanofluid (TiO2/water) on heat transfer characteristics such as the thermal conductivity coefficient, heat transfer coefficient, fuel clad, and fuel center temperatures in a VVER-1000 nuclear reactor are investigated. To this end, the cell equivalent of a fuel rod and its surrounding coolant fluid were obtained in the hexagonal fuel assembly of a VVER-1000 reactor. Then, a fuel rod was simulated in the hot channel using Computational Fluid Dynamics (CFD) simulation codes and thermohydraulic calculations (maximum fuel temperature, fluid outlet, Minimum Departure from Nucleate Boiling Ratio (MDNBR), etc.) were performed and compared with a VVER-1000 reactor without nanoparticles. One of the most important results of the analysis was that heat transfer and the thermal conductivity coefficient increased, and usage of the nanofluid reduced MDNBR.

  2. DNA methylation in porcine preimplantation embryos developed in-vivo or produced by in-vitro fertilization, parthenogenetic activation and somatic cell nuclear transfer

    DEFF Research Database (Denmark)

    Deshmukh, Rahul Shahaji; Østrup, Olga; Østrup, Esben;

    2011-01-01

    vitro fertilized (IVF), somatic cell nuclear transfer (SCNT) and parthenogenetically activated (PA) embryos were evaluated for DNA methylation quantification at different developmental stages. Fertilized (IV and IVF) one-cell stages lacked a substantial active demethylation of the paternal genome....... Embryos produced under in vitro conditions had higher levels of DNA methylation than IV. A lineage-specific DNA methylation (hypermethylation of the inner cell mass and hypomethylation of the trophectoderm) was observed in porcine IV late blastocysts, but was absent in PA- and SCNT-derived blastocysts...

  3. Communications Received from Certain Member States Regarding Guidelines for Transfers of Nuclear-related Dual-use Equipment, Materials, Software and Related Technology

    International Nuclear Information System (INIS)

    The Director General of the International Atomic Energy Agency has received Notes Verbales, dated 1 December 2005, from the Resident Representatives to the Agency of Argentina, Australia, Austria, Belarus, Belgium, Brazil, Bulgaria, Canada, Croatia, Czech Republic, Estonia, Finland, France, Germany, Greece, Hungary, Ireland, Italy, Japan, Republic of Korea, Latvia, Lithuania, Luxembourg, Malta, the Netherlands, New Zealand, Poland, Portugal, Slovenia, South Africa, Spain, Sweden, Switzerland, Turkey, Ukraine, the United Kingdom of Great Britain and Northern Ireland and the United States of America, relating to transfers of nuclear-related dual-use equipment, materials, software and related technology

  4. Communications received from certain Member States regarding guidelines for transfers of nuclear-related dual-use equipment, materials, software and related technology

    International Nuclear Information System (INIS)

    The document reproduces the text of the Notes Verbales received by the Director General of the IAEA on 20 October 1999 from the Resident Representatives to the Agency of Argentina, Australia, Austria, Belgium, Brazil, Bulgaria, Canada, the Czech Republic, Denmark, Finland, France, Germany, Hungary, Ireland, Italy, Japan, Republic of Korea, Latvia, the Netherlands, New Zealand, Norway, Poland, Portugal, Romania, the Slovak Republic, South Africa, Spain, Sweden, Switzerland, Ukraine, the United Kingdom, and the United States of America relating to the transfers of nuclear-related dual-use equipment, materials, software and related technology

  5. Expression of intracellular interferon-alpha confers antiviral properties in transfected bovine fetal fibroblasts and does not affect the full development of SCNT embryos.

    Directory of Open Access Journals (Sweden)

    Dawei Yu

    Full Text Available Foot-and-mouth disease, one of the most significant diseases of dairy herds, has substantial effects on farm economics, and currently, disease control measures are limited. In this study, we constructed a vector with a human interferon-α (hIFN-α (without secretory signal sequence gene cassette containing the immediate early promoter of human cytomegalovirus. Stably transfected bovine fetal fibroblasts were obtained by G418 selection, and hIFN-α transgenic embryos were produced by somatic cell nuclear transfer (SCNT. Forty-six transgenic embryos were transplanted into surrogate cows, and five cows (10.9% became pregnant. Two male cloned calves were born. Expression of hIFN-α was detected in transfected bovine fetal fibroblasts, transgenic SCNT embryos, and different tissues from a transgenic SCNT calf at two days old. In transfected bovine fetal fibroblasts, expression of intracellular IFN-α induced resistance to vesicular stomatitis virus infection, increased apoptosis, and induced the expression of double-stranded RNA-activated protein kinase gene (PKR and the 2'-5'-oligoadenylate synthetase gene (2'-5' OAS, which are IFN-inducible genes with antiviral activity. Analysis by qRT-PCR showed that the mRNA expression levels of PKR, 2'-5' OAS, and P53 were significantly increased in wild-type bovine fetal fibroblasts stimulated with extracellular recombinant human IFN-α-2b, showing that intracellular IFN-α induces biological functions similar to extracellular IFN-α. In conclusion, expression of intracellular hIFN-α conferred antiviral properties in transfected bovine fetal fibroblasts and did not significantly affect the full development of SCNT embryos. Thus, IFN-α transgenic technology may provide a revolutionary way to achieve elite breeding of livestock.

  6. Water-to-fish transfer of stable elements in near-coastal seas around a nuclear site of Korea

    International Nuclear Information System (INIS)

    In radiation dose assessment models for wildlife, the transfer of radionuclides to plant and animal species is usually quantified with concentration ratios (CRs) between them and environmental media. The IAEA recently produced a handbook of CR values covering various radionuclides and many kinds of plant and animal species. However, CR values can vary considerably with wildlife species and environmental conditions. Therefore, it is desirable to use as many site-specific CR data as possible. In the present study, the CR values of various stable elements as surrogates for radionuclides were investigated for several marine fish species inhabiting near-coastal seas around a nuclear power plant site of Korea. In May to June, 2012, fish and seawater samples were collected from six points in the sea within 10 km from Wolsung NPP site. For seawater samples, temperature, pH and salinity were measured immediately after collected. Fish samples were put in an icebox and carried to the laboratory, where they were freeze-dried and homogenized for the whole bodies using a grinder. Aliquots of the homogenized samples were changed into liquid samples as a result of chemical treatments. Sea water samples were filtered using a membrane filter (0.2 μm in pore size). Elemental concentrations were measured with the prepared liquid samples using the ICP-MS and ICP-AES. The CR value (L/kg-fresh) was expressed as the ratio of the fish concentration (Bq/kg-fresh) to the seawater concentration (Bq/L). The numbers of the elements and fish species investigated were 21 and 14, respectively. The CR values of Na and Mg were lower than 1 for all the fish species, whereas those of the remaining elements were always higher than 1 except for Li and U. Zn, Mn, Al and Fe formed a group of the highest CR values in general and their CR values mostly ranged from hundreds to thousands. In the case of Zn, CR values even higher than ten thousands were observed for seven species including greenling, sea bass

  7. Effect of leptin on oocyte maturation and subsequent pregnancy rate of cloned embryos reconstructed by somatic cell nuclear transfer in pigs

    Institute of Scientific and Technical Information of China (English)

    Hengxi Wei; Qiuyan Li; Jun Li; Yan Li; Yunping Dai; Yufang Ma; Kai Xue; Ning Li

    2008-01-01

    Cloning pigs by somatic cell nuclear transfer (SCNT) has wide applications in basic research,human medicine and agricultural production.To improve cloning efficiency,the effect of two basic maturation media,NCSU-23 and TCMI99,was compared,and TCM199 was selected for the following experiments with leptin.We systematically studied the effects of leptin supplementation on oocytes in vitro maturation (IVM),in vitro development of parthenogenetically activated (Phi) and SCNT embryos and/n vivo develop-ment of SCNT embryos after embryo transfer (ET).The results showed that supplementation of 100 or 200 ng/ml leptin into the mat-uration medium did not greatly affect nuclear maturation of oocytes,or cleavage rates of PA and SCNT (P<0.05).Blastocyst rates of PA and SCNT embryos were significantly improved when 100 or 200 ng/ml leptin was added to maturation medium,and the number of cells in PA blastocysts was also improved (P<0.05).The number of cells in blastocyst of SCNT was improved,when 100 ng/ml leptin was added (P<0.05).Furthermore,supplementation of 100 or 200 ng/ml leptin to the IVM medium may improve pregnancy rate and the delivery rate in pig cloning.

  8. Splitting and biopsy for bovine embryo sexing under field conditions.

    Science.gov (United States)

    Lopes, R F; Forell, F; Oliveira, A T; Rodrigues, J L

    2001-12-01

    Improvements on embryo micromanipulation techniques led to the use of embryo bisection technology in commercial embryo transfer programs, and made possible the direct genetic analysis of preimplantation bovine embryos by biopsy. For example, aspiration and microsection, allow bovine embryos sexing by detection of male-specific Y-chromosome in a sample of embryonic cells. We report on the application of the methodologies of splitting and biopsy of bovine embryos in field conditions, and on the results of embryo sex determination by the polymerase chain reaction (PCR). Pregnancy rates achieved with fresh bisected or biopsied embryos (50 to 60%) were similar to the fresh intact embryos (55 to 61%). The PCR protocol used for embryo sexing showed 92% to 94% of efficiency and 90 to 100% of accuracy. These results demonstrate these procedures are suitable for use in field conditions.

  9. In vitro developmental competence of pig nuclear transferred embryos: effects of GFP transfection, refrigeration, cell cycle synchronization and shapes of donor cells.

    Science.gov (United States)

    Zhang, Yun-Hai; Pan, Deng-Ke; Sun, Xiu-Zhu; Sun, Guo-Jie; Liu, Xiao-Hui; Wang, Xiao-Bo; Tian, Xing-Hua; Li, Yan; Dai, Yun-Ping; Li, Ning

    2006-08-01

    The present study was designed to evaluate the feasibility of producing pig transgenic blastocysts expressing enhanced green fluorescent protein (GFP) and to examine the effects of shape and preparation methods of donor cells on in vitro developmental ability of pig nuclear transferred embryos (NTEs). In experiment 1, the effect of GFP transfection on development of pig NTEs was evaluated. The cleavage and blastocyst rates showed no significant difference between NTEs derived from transfected and non-transfected donors. In experiment 2, the effect of different nuclear donor preparation methods on in vitro development of NTEs was examined. The cleavage rate showed no statistically significant differences among three preparation methods. The blastocyst rates of donor cells treated once at -4 degrees C and those of freshly digested cells were similar to each other (26.3% vs 17.9%). The lowest blastocyst rates (5.88%) were observed when cells cryopreserved at -196 degrees C were used as donors. In experiment 3, the effect of different cell cycle synchronization methods on the in vitro development potential of pig NTEs was evaluated. The cleavage rate of NTEs derived from cycling cells was much better than that of NTEs derived from serum-starved cells (64.4% vs 50.5%, p refrigerated pig GFP-transfected cells could be used as donors in nuclear transfer and these NTEs could be effectively developed to blastocyst stage; (ii) serum starvation of GFP-transfected cells is not required for preimplantation development of pig NTEs; and (iii) a rough surface of GFP-transfected donor cells affects fusion rate negatively but has no influence on the cleavage rate or blastocyst rate of pig NTEs. PMID:16822335

  10. Analysis of interactions of mechanical deformations and mass transfer on heat transfer from an underground nuclear-waste repository. Final report

    International Nuclear Information System (INIS)

    A review of existing models identified several effects that may need consideration in further model development. Most of these effects involved coupling equations through variable property values rather than through omission of any significant mechanism. However, it was also shown that more than one mechanism may adequately simulate a given set of experimental data and additional experimental data are needed to establish which (if any) of the possible mechanisms would actually control conditions in a nuclear waste repository. In particular, it is believed that mathematical modeling of major thermomechanical effects can be accomplished with finite element analysis computer programs, provided that adequate thermomechanical property data of salt and overburden are attained. An attempt was made to develop a general set of differential equations for simulating momentum, mass, and energy flows in geologic formations in order to illustrate the possible mechanisms and point out those included and not included in existing models. Most of the mechanisms are included in some manner in existing models although some approximations may not be adequate. More experimental data are required to assess the importance of most omitted mechanisms. Analysis of some data on brine migration in salt indicated that two mechanisms, acting simultaneously, could adequately explain the flow. These are Darcy flow and a combination of ordinary and thermal diffusion enhanced by temperature-dependent solubility. Equations based on this simultaneous action correlated the data very well and indicated the possible need to include both (and, maybe other) mechanisms in future models. A program is recommended for further study of brine mobility. An expected result of this program includes recommendations for further experimental work

  11. Analysis of interactions of mechanical deformations and mass transfer on heat transfer from an underground nuclear-waste repository. Final report

    Energy Technology Data Exchange (ETDEWEB)

    Bloom, S.G.; Hulbert, L.E.

    1979-10-01

    A review of existing models identified several effects that may need consideration in further model development. Most of these effects involved coupling equations through variable property values rather than through omission of any significant mechanism. However, it was also shown that more than one mechanism may adequately simulate a given set of experimental data and additional experimental data are needed to establish which (if any) of the possible mechanisms would actually control conditions in a nuclear waste repository. In particular, it is believed that mathematical modeling of major thermomechanical effects can be accomplished with finite element analysis computer programs, provided that adequate thermomechanical property data of salt and overburden are attained. An attempt was made to develop a general set of differential equations for simulating momentum, mass, and energy flows in geologic formations in order to illustrate the possible mechanisms and point out those included and not included in existing models. Most of the mechanisms are included in some manner in existing models although some approximations may not be adequate. More experimental data are required to assess the importance of most omitted mechanisms. Analysis of some data on brine migration in salt indicated that two mechanisms, acting simultaneously, could adequately explain the flow. These are Darcy flow and a combination of ordinary and thermal diffusion enhanced by temperature-dependent solubility. Equations based on this simultaneous action correlated the data very well and indicated the possible need to include both (and, maybe other) mechanisms in future models. A program is recommended for further study of brine mobility. An expected result of this program includes recommendations for further experimental work.

  12. Effects of transient and non-uniform distribution of heat flux on intensity of heat transfer and burnout conditions in the channels of nuclear reactors

    Energy Technology Data Exchange (ETDEWEB)

    Vitaly Osmachkin [Russian Research Center ' Kurchatov Institute' 1, Kurchatov sq, Moscow 123182 (Russian Federation)

    2005-07-01

    Full text of publication follows: The influence of power transient, changes of flow rate, inlet temperatures or pressure in cores of nuclear reactors on heat transfer and burnout conditions in channels depend on rate of such violations. Non-uniform distribution of the heat flux is also important factor for heat transfer and development of crisis phenomenon. Such effects may be significant for NPPs safety. But they have not yet generally accepted interpretation. Steady state approach is often recommended for use in calculations. In the paper a review of experimental observed so-called non-equilibrium effects is presented. The effects of space and time factors are displaying due delay in reformation turbulence intensity, velocity, temperatures or void fraction profiles, water film flow on the surface of heated channels. For estimation of such effect different methods are used. Modern computer codes based on two or three fluids approaches are considered as most effective. But simple and clear correlations may light up the mechanics of effects on heat transfer and improve general understanding of scale and significance of the transient events. In the paper the simplified methods for assessment the influence of lags in the development of distributions of parameters of flow, the relaxation of temporal or space violations are considered. They are compared with more sophisticated approaches. Velocities of disturbance fronts moving along the channels are discussed also. (author)

  13. Characterization and regulation of the bovine stearoyl-CoA desaturase gene promoter

    International Nuclear Information System (INIS)

    The bovine stearoyl-CoA desaturase (Scd) gene plays an important role in the bovine mammary gland where substrates such as stearic and vaccenic acids are converted to oleic acid and conjugated linoleic acid (CLA), respectively. Up to 90% of the CLA in bovine milk is formed due to the action of this enzyme in the mammary gland. The areas of the bovine promoter of importance in regulating this key enzyme were examined and an area of 36 bp in length was identified as having a critical role in transcriptional activation and is designated the Scd transcriptional enhancer element (STE). Electrophoretic mobility shift assay detected three binding complexes on this area in Mac-T cell nuclear extracts. Treatment of cells with CLA caused a significant reduction in transcriptional activity, with this effect being mediated through the STE region. The bovine Scd gene promoter was up-regulated by insulin and down-regulated by oleic acid

  14. Bovine coronavirus hemagglutinin protein.

    Science.gov (United States)

    King, B; Potts, B J; Brian, D A

    1985-02-01

    Treatment of purified bovine coronavirus (Mebus strain) with pronase destroyed the integrity of virion surface glycoproteins gp140, gp120, gp100, reduced the amount of gp26 and destroyed the hemagglutinating activity of the virus. Bromelain, on the other hand, destroyed the integrity of gp120, gp100 and gp26 but failed to remove gp140 and failed to destroy viral hemagglutinating activity. These experiments suggest that gp140 is the virion hemagglutinin. Immunoblotting studies using monospecific antiserum demonstrate that gp140 is a disulfide-linked dimeric structure reducible to monomers of 65 kDa.

  15. Camel and bovine chymosin

    DEFF Research Database (Denmark)

    Jensen, Jesper Langholm; Mølgaard, Anne; Poulsen, Jens-Christian Navarro;

    2013-01-01

    Bovine and camel chymosin are aspartic peptidases that are used industrially in cheese production. They cleave the Phe105-Met106 bond of the milk protein κ-casein, releasing its predominantly negatively charged C-terminus, which leads to the separation of the milk into curds and whey. Despite...... chymosin. Both enzymes possess local positively charged patches on their surface that can play a role in interactions with the overall negatively charged C-terminus of κ-casein. Camel chymosin contains two additional positive patches that favour interaction with the substrate. The improved electrostatic...

  16. Bovine Virus Diarrhea (BVD)

    OpenAIRE

    Hoar, Bruce R.

    2004-01-01

    Bovine virus diarrhea (BVD) is a complicated disease to discuss as it can result in a wide variety of disease problems from very mild to very severe. BVD can be one of the most devastating diseases cattle encounter and one of the hardest to get rid of when it attacks a herd. The viruses that cause BVD have been grouped into two genotypes, Type I and Type II. The disease syndrome caused by the two genotypes is basically the same, however disease caused by Type II infection is often more severe...

  17. Proteomic Analysis of Bovine Nucleolus

    Institute of Scientific and Technical Information of China (English)

    Amrutlal K.Patel; Doug Olson; Suresh K. Tikoo

    2010-01-01

    Nucleolus is the most prominent subnuclear structure, which performs a wide variety of functions in the eu-karyotic cellular processes. In order to understand the structural and functional role of the nucleoli in bovine cells,we analyzed the proteomie composition of the bovine nueleoli. The nucleoli were isolated from Madin Darby bo-vine kidney cells and subjected to proteomie analysis by LC-MS/MS after fractionation by SDS-PAGE and strongcation exchange chromatography. Analysis of the data using the Mascot database search and the GPM databasesearch identified 311 proteins in the bovine nucleoli, which contained 22 proteins previously not identified in theproteomic analysis of human nucleoli. Analysis of the identified proteins using the GoMiner software suggestedthat the bovine nueleoli contained proteins involved in ribosomal biogenesis, cell cycle control, transcriptional,translational and post-translational regulation, transport, and structural organization.

  18. Taking a fresh Look at boiling heat transfer on the road to improved nuclear economics and efficiency

    Energy Technology Data Exchange (ETDEWEB)

    Baglietto, E.; Pointer, W. D.

    2016-08-01

    In the effort to reinvigorate innovation in the way we design, build, and operate the nuclear power generating stations of today and tomorrow, nothing can be taken for granted. Not even the seemingly familiar physics of boiling water. The Consortium for the Advanced Simulation of Light Water Reactors, or CASL, is focused on the deployment of advanced modeling and simulation capabilities to enable the nuclear industry to reduce uncertainties in the prediction of multi-physics phenomena and continue to improve the performance of todays light water reactors and their fuel. An important part of the CASL mission is the development of a next generation thermal hydraulics simulation capability, integrating the history of engineering models based on based on experimental experience with the computing technology of the future. (Author)

  19. Viral infections and bovine mastitis: a review

    NARCIS (Netherlands)

    Wellenberg, G.J.; Poel, van der W.H.M.; Oirschot, van J.T.

    2002-01-01

    This review deals with the role of viruses in the aetiology of bovine mastitis. Bovine herpesvirus 1, bovine herpesvirus 4, foot-and-mouth disease virus, and parainfluenza 3 virus have been isolated from milk from cows with clinical mastitis. Intramammary inoculations of bovine herpesvirus 1 or para

  20. Study on the interaction of bovine serum albumin and fleroxacin by fluorescence method

    International Nuclear Information System (INIS)

    A fluorescence method is used to study the fluorescence quenching of bovine serum albumin by its interaction with fleroxacin. The interaction association constants of bovine serum albumin and fleroxacin are determined from a double reciprocal Lineweaver-Burk plot. According to the Foester dipole-dipole energy transfer, the distance to be measured between the fleroxacin and tryptophane is 4.37 nm. From thermodynamical coordination it can be judged that the binding power between fleroxacin and bovine serum albumin is static electric power

  1. Synergistic Effect of Insulin on in vitro Development of Immature Bovine Oocytes

    OpenAIRE

    Mojtaba Dashtizad; Abd W. Haron; Rosnina Yusoff; Morteza Daliri; Hadi Hajarian; Mehdi Najari; Yap K. Chee; Abas M. Othman

    2010-01-01

    Problem statement: Development of efficient culture system to support embryonic development would be valuable when quality of produced embryos was important. However, the rate of bovine embryo production in vitro was still lower than expected. Present study, including of three experiments, was carried out to investigate the effect of insulin on nuclear maturation and subsequent development of immature bovine oocytes and in vitro fertilized embryos. Approach: Grade one cumulus-oocyte-complexes...

  2. Quality and safety of bovine clones and their products.

    Science.gov (United States)

    Heyman, Y; Chavatte-Palmer, P; Fromentin, G; Berthelot, V; Jurie, C; Bas, P; Dubarry, M; Mialot, J P; Remy, D; Richard, C; Martignat, L; Vignon, X; Renard, J P

    2007-08-01

    cattle derived from somatic nuclear transfer than in cattle born from conventional reproduction. Our results confirm that the quality and safety of products (milk and meat) from adult and clinically healthy cloned cattle is globally similar to normal animals. However, from a strictly biological point of view, the slightly delayed maturation we observed in the muscle of clones together with some marginal differences identified in FA composition of both muscle and milk, point to the need for more refined analysis to totally exclude any risks from the consumption of those products.

  3. Identification of Valid Housekeeping Genes for Real-Time Quantitative PCR Analysis of Collapsed Lung Tissues of Neonatal Somatic Cell Nuclear Transfer-Derived Cattle

    DEFF Research Database (Denmark)

    Liu, Yan; Zhang, Yunhai; Jiang, Qiuling;

    2015-01-01

    Cloned calves produced by somatic cell nuclear transfer frequently suffer alveolar collapse as newborns. To study the underlying pathophysiological mechanisms responsible for this phenomenon, the expression profiles of numerous genes involved in lung development need to be investigated......), peptidylprolyl isomerase A (PPIA), glyceraldehyde-3-phosphate dehydrogenase (GAPDH), TATA-box binding protein (TBP), and 5.8S ribosomal RNA (5.8S rRNA)-were selected and evaluated as candidates. Their gene expression levels in the collapsed lungs of deceased neonate cloned calves and normal lung derived from...... genes by NormFinder. Taking these results into account, we conclude that 5.8S rRNA and PPIA could be the most reliable reference genes for studying the genes involved in alveolar collapse. Moreover, 5.8S rRNA could be represented as a uniform reference gene in similar cases....

  4. Proliferating cell nuclear antigen (PCNA) interacts with a meiosis-specific RecA homologues, Lim15/Dmc1, but does not stimulate its strand transfer activity

    International Nuclear Information System (INIS)

    PCNA is a multi-functional protein that is involved in various nuclear events. Here we show that PCNA participates in events occurring during early meiotic prophase. Analysis of protein-protein interactions using surface plasmon resonance indicates that Coprinus cinereus PCNA (CoPCNA) specifically interacts with a meiotic specific RecA-like factor, C. cinereus Lim15/Dmc1 (CoLim15) in vitro. The binding efficiency increases with addition of Mg2+ ions, while ATP inhibits the interaction. Co-immunoprecipitation experiments indicate that the CoLim15 protein interacts with the CoPCNA protein in vitro and in the cell extracts. Despite the interaction between these two factors, no enhancement of CoLim15-dependent strand transfer activity by CoPCNA was found in vitro. We propose that the interaction between Lim15/Dmc1 and PCNA mediates the recombination-associated DNA synthesis during meiosis

  5. Four-electron model for singlet and triplet excitation energy transfers with inclusion of coherence memory, inelastic tunneling and nuclear quantum effects

    Science.gov (United States)

    Suzuki, Yosuke; Ebina, Kuniyoshi; Tanaka, Shigenori

    2016-08-01

    A computational scheme to describe the coherent dynamics of excitation energy transfer (EET) in molecular systems is proposed on the basis of generalized master equations with memory kernels. This formalism takes into account those physical effects in electron-bath coupling system such as the spin symmetry of excitons, the inelastic electron tunneling and the quantum features of nuclear motions, thus providing a theoretical framework to perform an ab initio description of EET through molecular simulations for evaluating the spectral density and the temporal correlation function of electronic coupling. Some test calculations have then been carried out to investigate the dependence of exciton population dynamics on coherence memory, inelastic tunneling correlation time, magnitude of electronic coupling, quantum correction to temporal correlation function, reorganization energy and energy gap.

  6. DNA methylation in porcine preimplantation embryos developed in vivo or produced by in vitro fertilization, parthenogenetic activation and somatic cell nuclear transfer

    DEFF Research Database (Denmark)

    Deshmukh, Rahul Shahaji; Østrup, Olga; Østrup, Esben;

    2011-01-01

    ), in vitro fertilized (IVF), somatic cell nuclear transfer (SCNT) and parthenogenetically activated (PA) embryos were evaluated for DNA methylation quantification at different developmental stages. Fertilized (IV and IVF) one-cell stages lacked a substantial active demethylation of the paternal genome....... Embryos produced under in vitro conditions had higher levels of DNA methylation than IV. A lineage-specific DNA methylation (hypermethylation of the inner cell mass and hypomethylation of the trophectoderm) was observed in porcine IV late blastocysts, but was absent in PA- and SCNT-derived blastocysts...... despite the occurrence of de novo methylation in early blastocysts. Comparable levels of DNA methylation were found in IV embryos and in 50% and 14% of SCNT early and late blastocysts, respectively. In conclusion, DNA methylation patterns were adversely affected by in vitro embryo production...

  7. Comparative pluripotency analysis of mouse embryonic stem cells derived from wild-type and infertile hermaphrodite somatic cell nuclear transfer blastocysts

    Institute of Scientific and Technical Information of China (English)

    FAN Yong; YAO RuQiang; YU Yang; LI ZanDong; WANG Liu; ALICE Jouneau; ZHOU Qi; TONG Man; ZHAO ChunLi; DING ChenHui; HAO Jie; LV Zhuo; DAI XiangPeng; HAI Tang; LI XueMei

    2008-01-01

    Therapeutic cloning, whereby embryonic stem cells (ESCs) are derived from patient-specific cloned blastocysts via somatic cell nuclear transfer (SCNT), holds great promise for treating many human diseases using regenerative medicine. Teratoma formation and germline transmission have been used to confirm the pluripotency of mouse stem cells, but human embryonic stem cells (hESCs) have not been proven to be fully pluripotent owing to the ethical impossibility of testing for germ line transmission, which would be the strongest evidence for full pluripotency. Therefore, formation of differentiated cells from the three somatic germ layers within a teratoma is taken as the best indicator of pluripotency in hESC lines. The possibility that these lines lack full multi- or pluripotency has not yet been evaluated.In this study, we established 16 mouse ESC lines, including 3 genetically defective nuclear transfer-ESC (ntESC) lines derived from SCNT blastocysts of infertile hermaphrodite F1 mice and 13 ntESC lines derived from SCNT blastocysts of normal F1 mice. We found that the defective ntESCs expressed all in vitro markers of pluripotency and could form teratomas that included derivatives from all three germ layers, but could not be transmitted via the germ line, in contrast with normal ntESCs. Our results indicate that teratoma formation assays with hESCs might be an insufficient standard to assess full pluripotency, although they do define multipotency to some degree. More rigorous standards are required to assess the safety of hESCs for therapeutic cloning.

  8. Prospects of international collaboration and knowledge transfer on project management and public relations in nuclear power engineering (economic aspect)

    International Nuclear Information System (INIS)

    In this paper author deals with public consultations of the State Scientific Engineering Center of Control Systems and Emergency Response, Kiev. The following conclusions should be made: - Public consultations support and strengthen the positive image of nuclear energy projects, also by youth. - The public relations work which includes only occasional meetings or discussions of some problems is not effective. - Effective are the following facilities: - press releases in popular scientific and technical issues (now there are a few of such issues because of lack of financing) - career-guidance at all phases of education (to begin with secondary schools to post-graduate courses). It is well known that the economic situation in a country influences the functioning of electric energy market and efficiency of energy companies. The aim of our research is to find out the ways of further development of energy branch and what economic consequences the different changes in legislation and taxation systems can bring about. Great attention is paid to the activities of the producers of nuclear energy and regional energy supplying companies and the state of payments in electric energy market. Current research is being carried out to find out the ways of enlarging current assets, decreasing debts, also optimal equipment depreciation normative and main dependencies between some economic indexes and existing tariffs. All these tasks can be effectively solved using the. (author)

  9. Inter-organisational knowledge transfer: building and sustaining the sources of innovation in nuclear safety and security

    International Nuclear Information System (INIS)

    The current complexity of innovation processes has led to an understanding that the models of innovation have changed from linear model to a model characterised by multiple interactions and complex networks. Within this more multifaceted environment, has emerged a new set of actors, generally termed as intermediaries, performing a variety of tasks in the innovation process. The innovation literature has recognised various important supporting activities performed by intermediaries, by linking and facilitating the movement of information and knowledge between actors within an innovation system, in order to fill information gaps. Complementary, we make the assumption that the intermediary can assume a more central role in the innovation process, performing activities beyond to filling information gaps, since they intervene to create, prioritise, and articulate meaning to practices. Under this argument, this paper explores how intermediaries work in making innovation happen in the Brazilian nuclear safety and security area, demonstrating the influence of intermediary organisations in improving nuclear regulatory activities. We make sense of these processes by analyzing intermediary roles in the recent regulatory activities improvements, specifically those related to the practices involving radiation sources in medicine. Thus, through an empirical case study, this paper examines the issue of intermediation in a wide sense, including strategic activities preformed by intermediaries, associated with accessing, diffusing, coordinating and enabling knowledge activities. (author)

  10. Inter-organisational knowledge transfer: building and sustaining the sources of innovation in nuclear safety and security

    Energy Technology Data Exchange (ETDEWEB)

    Staude, Fabio, E-mail: fstaude@cnen.gov.br [Comissao Nacional de Energia Nuclear (CNEN-RJ), Rio de Janeiro, RJ (Brazil); Ramirez, Matias, E-mail: matias.ramirez@sussex.ac.uk [University of Sussex (SPRU/US), Brighton (United Kingdom). Science and Technology Policy Research

    2013-07-01

    The current complexity of innovation processes has led to an understanding that the models of innovation have changed from linear model to a model characterised by multiple interactions and complex networks. Within this more multifaceted environment, has emerged a new set of actors, generally termed as intermediaries, performing a variety of tasks in the innovation process. The innovation literature has recognised various important supporting activities performed by intermediaries, by linking and facilitating the movement of information and knowledge between actors within an innovation system, in order to fill information gaps. Complementary, we make the assumption that the intermediary can assume a more central role in the innovation process, performing activities beyond to filling information gaps, since they intervene to create, prioritise, and articulate meaning to practices. Under this argument, this paper explores how intermediaries work in making innovation happen in the Brazilian nuclear safety and security area, demonstrating the influence of intermediary organisations in improving nuclear regulatory activities. We make sense of these processes by analyzing intermediary roles in the recent regulatory activities improvements, specifically those related to the practices involving radiation sources in medicine. Thus, through an empirical case study, this paper examines the issue of intermediation in a wide sense, including strategic activities preformed by intermediaries, associated with accessing, diffusing, coordinating and enabling knowledge activities. (author)

  11. Influence of heat transfer on walls due to aerosol decomposition rate in the containment building of nuclear power plants during heavy incidents

    International Nuclear Information System (INIS)

    Today, German nuclear power plants are leading in safety standards worldwide. Increasing potentials arise continuously along with improvements in technology. One of these potentials is the best-estimate simulation of fission product transport in case of a severe accident. A main part of the fission products is allocated on aerosols. Therefore, the aerosol behavior before containment leakage is important for the radioactive source term to the environment. Having a good knowledge about the main aerosol phenomena, it is possible to simulate them numerically. This enables to develop and test safety measures to limit damages before accidents occur. Within this study, the main aerosol phenomena have been ascertained and accordingly classified into formation, transport and reduction. On this basis, simulations of one- and multi-component aerosol experiments of the KAEVER series have been performed with the COCOSYS code. Due to an overprediction of the computed volume condensation rate, the results showed an overestimation of the reduction rate of insoluble aerosols. The reason was found to be the underestimation of the wall condensation rate. Based on an additional plain thermal hydraulic multi compartment experiment, these uncertainties in the wall heat transfer correlations were investigated in detail. The results show a strong dependency between the wall condensation rate and the convective heat transfer, resp. the characteristic length. In case of mainly forced convection, correct values for the characteristic length led to an underestimation of the calculated heat transfer coefficients. The analysis of the heat transfer models show an inconsistency in the coupling of free and forced convection. Therefore, an improved and consistent convection model has been developed and implemented. Both models have been tested on different experiments. Although the new model shows only minor improvements, it could be proven that the influence for forced convection is significant

  12. Analysis of the effects of explosion of a hydrogen cylinder on the transfer of radioactive liquid wastes at nuclear power stations

    Energy Technology Data Exchange (ETDEWEB)

    Lopes, Karina B., E-mail: karina@inb.gov.b [Industrias Nucleares do Brasil (INB), Rio de Janeiro, RJ (Brazil); Melo, Paulo Fernando F.F. e, E-mail: frutoso@nuclear.ufrj.b [Universidade Federal do Rio de Janeiro (PEN/COPPE/UFRJ), RJ (Brazil). Coordenacao dos Programas de Pos-Graduacao de Engenharia. Programa de Engenharia Nuclear

    2011-07-01

    This work presents a study of explosion effects of a stored hydrogen cylinder on the transfer of radioactive liquid wastes at nuclear power plants. The peak overpressure is calculated, as well as the strength of resulting fragments, thus confirming the main harmful effect of an explosion of flammable vapor cloud, based on the TNT equivalent method. The scenarios identified are calculated and compared with the overpressure ranges of 1%, 50% and 99% of structural damages, which were determined by the Eisenberg's vulnerability model. The results show that the overpressure and the resulting fragments from the explosion of a hydrogen gas cylinder are not able to cause the overturning of the tanker under study, and also show that a minimum distance of 30 meters between the hydrogen cylinder and the tanker can be considered a safe distance to the passage of this tanker during the transfer of radioactive liquid waste, in which the likelihood of occurrence of structural damages is less than 1%. (author)

  13. 8. Innovative Technologies: Two-Phase Heat Transfer in Water-Based Nanofluids for Nuclear Applications. Final Report

    International Nuclear Information System (INIS)

    Nanofluids are colloidal dispersions of nanoparticles in water. Many studies have reported very significant enhancement (up to 200%) of the Critical Heat Flux (CHF) in pool boiling of nanofluids (You et al. 2003, Vassallo et al. 2004, Bang and Chang 2005, Kim et al. 2006, Kim et al. 2007). These observations have generated considerable interest in nanofluids as potential coolants for more compact and efficient thermal management systems. Potential Light Water Reactor applications include the primary coolant, safety systems and severe accident management strategies, as reported in other papers (Buongiorno et al. 2008 and 2009). However, the situation of interest in reactor applications is often flow boiling, for which no nanofluid data have been reported so far. In this project we investigated the potential of nanofluids to enhance CHF in flow boiling. Subcooled flow boiling heat transfer and CHF experiments were performed with low concentrations of alumina, zinc oxide, and diamond nanoparticles in water ((le) 0.1% by volume) at atmospheric pressure. It was found that for comparable test conditions the values of the nanofluid and water heat transfer coefficient (HTC) are similar (within ±20%). The HTC increased with mass flux and heat flux for water and nanofluids alike, as expected in flow boiling. The CHF tests were conducted at 0.1 MPa and at three different mass fluxes (1500, 2000, 2500 kg/m2s) under subcooled conditions. The maximum CHF enhancement was 53%, 53% and 38% for alumina, zinc oxide and diamond, respectively, always obtained at the highest mass flux. A post-mortem analysis of the boiling surface reveals that its morphology is altered by deposition of the particles during nanofluids boiling. A confocal-microscopy-based examination of the test section revealed that nanoparticles deposition not only changes the number of micro-cavities on the surface, but also the surface wettability. A simple model was used to estimate the ensuing nucleation site

  14. 8. Innovative Technologies: Two-Phase Heat Transfer in Water-Based Nanofluids for Nuclear Applications Final Report

    Energy Technology Data Exchange (ETDEWEB)

    Buongiorno, Jacopo; Hu, Lin-wen

    2009-07-31

    Nanofluids are colloidal dispersions of nanoparticles in water. Many studies have reported very significant enhancement (up to 200%) of the Critical Heat Flux (CHF) in pool boiling of nanofluids (You et al. 2003, Vassallo et al. 2004, Bang and Chang 2005, Kim et al. 2006, Kim et al. 2007). These observations have generated considerable interest in nanofluids as potential coolants for more compact and efficient thermal management systems. Potential Light Water Reactor applications include the primary coolant, safety systems and severe accident management strategies, as reported in other papers (Buongiorno et al. 2008 and 2009). However, the situation of interest in reactor applications is often flow boiling, for which no nanofluid data have been reported so far. In this project we investigated the potential of nanofluids to enhance CHF in flow boiling. Subcooled flow boiling heat transfer and CHF experiments were performed with low concentrations of alumina, zinc oxide, and diamond nanoparticles in water (≤ 0.1 % by volume) at atmospheric pressure. It was found that for comparable test conditions the values of the nanofluid and water heat transfer coefficient (HTC) are similar (within ±20%). The HTC increased with mass flux and heat flux for water and nanofluids alike, as expected in flow boiling. The CHF tests were conducted at 0.1 MPa and at three different mass fluxes (1500, 2000, 2500 kg/m2s) under subcooled conditions. The maximum CHF enhancement was 53%, 53% and 38% for alumina, zinc oxide and diamond, respectively, always obtained at the highest mass flux. A post-mortem analysis of the boiling surface reveals that its morphology is altered by deposition of the particles during nanofluids boiling. A confocal-microscopy-based examination of the test section revealed that nanoparticles deposition not only changes the number of micro-cavities on the surface, but also the surface wettability. A simple model was used to estimate the ensuing nucleation site

  15. The host defense proteome of human and bovine milk.

    Directory of Open Access Journals (Sweden)

    Kasper Hettinga

    Full Text Available Milk is the single source of nutrients for the newborn mammal. The composition of milk of different mammals has been adapted during evolution of the species to fulfill the needs of the offspring. Milk not only provides nutrients, but it also serves as a medium for transfer of host defense components to the offspring. The host defense proteins in the milk of different mammalian species are expected to reveal signatures of evolution. The aim of this study is therefore to study the difference in the host defense proteome of human and bovine milk. We analyzed human and bovine milk using a shot-gun proteomics approach focusing on host defense-related proteins. In total, 268 proteins in human milk and 269 proteins in bovine milk were identified. Of these, 44 from human milk and 51 from bovine milk are related to the host defense system. Of these proteins, 33 were found in both species but with significantly different quantities. High concentrations of proteins involved in the mucosal immune system, immunoglobulin A, CD14, lactoferrin, and lysozyme, were present in human milk. The human newborn is known to be deficient for at least two of these proteins (immunoglobulin A and CD14. On the other hand, antimicrobial proteins (5 cathelicidins and lactoperoxidase were abundant in bovine milk. The high concentration of lactoperoxidase is probably linked to the high amount of thiocyanate in the plant-based diet of cows. This first detailed analysis of host defense proteins in human and bovine milk is an important step in understanding the function of milk in the development of the immune system of these two mammals.

  16. Process and container system for transferring or transporting fuel elements from a nuclear power station to a store

    International Nuclear Information System (INIS)

    A system of containers with three types of containers (an inside container, a transport container and a storage container) is used. One either sets the inside container open on the lid side into the transport container first in the water pond of the nuclear power station, and one then sets the fuel elements into the inside container, or one places the inside container, loaded with fuel elements away from the transport container, into the transport container. Both containers are then closed and are transported to the store as a unit. The storage container open on the lid side is prepared there, the floor of the transport container is opened and this, together with the inside container, is lifted above the storage container or set above the storage container. The inside container is then lowered onto the storage container, the transport container is removed and the lid of the storage container is closed. (orig./HP)

  17. A dynamic model to calculate cadmium concentrations in bovine tissues from basic soil characteristics

    Energy Technology Data Exchange (ETDEWEB)

    Waegeneers, Nadia, E-mail: nadia.waegeneers@var.fgov.be; Ruttens, Ann; De Temmerman, Ludwig

    2011-06-15

    A chain model was developed to calculate the flow of cadmium from soil, drinking water and feed towards bovine tissues. The data used for model development were tissue Cd concentrations of 57 bovines and Cd concentrations in soil, feed and drinking water, sampled at the farms were the bovines were reared. Validation of the model occurred with a second set of measured tissue Cd concentrations of 93 bovines of which age and farm location were known. The exposure part of the chain model consists of two parts: (1) a soil-plant transfer model, deriving cadmium concentrations in feed from basic soil characteristics (pH and organic matter content) and soil Cd concentrations, and (2) bovine intake calculations, based on typical feed and water consumption patterns for cattle and Cd concentrations in feed and drinking water. The output of the exposure model is an animal-specific average daily Cd intake, which is then taken forward to a kinetic uptake model in which time-dependent Cd concentrations in bovine tissues are calculated. The chain model was able to account for 65%, 42% and 32% of the variation in observed kidney, liver and meat Cd concentrations in the validation study. - Research highlights: {yields} Cadmium transfer from soil, drinking water and feed to bovine tissues was modeled. {yields} The model was based on 57 bovines and corresponding feed and soil Cd concentrations. {yields} The model was validated with an independent data set of 93 bovines. {yields} The model explained 65% of variation in kidney Cd in the validation study.

  18. Effect of growth factors on oocyte maturation and allocations of inner cell mass and trophectoderm cells of cloned bovine embryos.

    Science.gov (United States)

    Arat, Sezen; Caputcu, Arzu Tas; Cevik, Mesut; Akkoc, Tolga; Cetinkaya, Gaye; Bagis, Haydar

    2016-08-01

    This study was conducted to determine the additive effects of exogenous growth factors during in vitro oocyte maturation (IVM) and the sequential culture of nuclear transfer (NT) embryos. Oocyte maturation and culture of reconstructed embryos derived from bovine granulosa cells were performed in culture medium supplemented with either epidermal growth factor (EGF) alone or a combination of EGF with insulin-like growth factor-I (IGF-I). The maturation rates of oocytes matured in the presence of EGF or the EGF + IGF-I combination were significantly higher than those of oocytes matured in the presence of only fetal calf serum (FCS) (P 0.05). IGF-I alone or in combination with EGF in sequential embryo culture medium significantly increased the ratio of inner cell mass (ICM) to total blastocyst cells (P media of cloned bovine embryos increased the ICM without changing the total cell number. These unknown and uncontrolled effects of growth factors can alter the allocation of ICM and trophectoderm cells (TE) in NT embryos. A decrease in TE cell numbers could be a reason for developmental abnormalities in embryos in the cloning system. PMID:26444069

  19. New insight into the stereoselective interactions of quinine and quinidine, with bovine serum albumin.

    Science.gov (United States)

    Liu, Yan; Chen, Mingmao; Wang, Shuaihua; Lin, Jingjing; Cai, Lizhen; Song, Ling

    2014-05-01

    Quinine (QN) and quinidine (QD), the chief quinoline alkaloids of various species of cinchona bark, are stereoisomers to each other. In this study, a series of appropriate and efficient methods have been applied to compare the binding modes of QN and QD with bovine serum albumin (BSA). The isothermal titration calorimetry and room temperature phosphorescence results show that both QN and QD can interact with BSA at one binding site to form drug-protein complexes, mainly through enthalpic driving force with the binding affinity order: QN > QD. The fluorescence resonance energy transfer and time-resolved fluorescence spectroscopy exhibits that QN has a larger energy transfer and more intensified binding capacity for BSA than QD. Data of dynamic light scattering reveal that the aggregate state of BSA is changed during this binding process, and the particle size distribution of QN-BSA bioconjugate is larger than that of QD. Nuclear magnetic resonance analysis indicates that aromatic protons make more contribution during ligand-protein complexation than that of aliphatic protons. The circular dichroism spectra exhibit different degrees of changes in BSA secondary structures in the presence of QN and QD, respectively.

  20. 31P-saturation-transfer nuclear-magnetic-resonance measurements of phosphocreatine turnover in guinea-pig brain slices.

    Science.gov (United States)

    Morris, P G; Feeney, J; Cox, D W; Bachelard, H S

    1985-05-01

    The technique of 31P saturation-transfer n.m.r. was used to determine the forward and the reverse rate constants of creatine phosphotransferase in superfused guinea-pig cerebral tissues in vitro. The calculated forward rate constant of 0.22 +/- 0.03s-1 compared well with a previously reported value for rat brain in vivo [Shoubridge, Briggs & Radda (1982) FEBS Lett. 140, 288-292]. The reverse rate constant was found to be 0.55 +/- 0.10s-1. 3. By using concentrations of ATP and phosphocreatine estimated previously for this superfused preparation [Cox, Morris, Feeney & Bachelard (1983) Biochem. J. 212, 365-370], forward and reverse flux rates were calculated to be 0.68 and 0.72 mumol X s-1 X g-1 respectively. The concordance of forward and reverse fluxes contrasts with the situation observed in vitro in other tissues, and suggests that the creatine phosphotransferase reaction is at equilibrium under the conditions used here. 4. Lowering the concentration of glucose in the superfusing medium from 10mM to 0.5mM had no significant effect on phosphocreatine concentration or on the forward (ATP-generating) flux through creatine phosphotransferase. The results indicate that a normal phosphocreatine content in the presence of lowered glucose availability is reflected by an unchanged turnover rate.

  1. Combination of the somatic cell nuclear transfer method and RNAi technology for the production of a prion gene-knockdown calf using plasmid vectors harboring the U6 or tRNA promoter.

    Science.gov (United States)

    Wongsrikeao, Pimprapar; Sutou, Shizuyo; Kunishi, Miho; Dong, Ya Juan; Bai, Xuejin; Otoi, Takeshige

    2011-01-01

    By combining RNAi technology with SCNT method, we attempted to produce transgenic calves with knocked down bPRNP for technological assessments. The respective utilities of type II (tRNA) and type III (hU6) Pol III promoters in mediating plasmid vector-based RNAi for the production of a bPRNP-knockdown calf were compared. Plasmid harboring DNA for siRNA expression was introduced stably into the genome of primary cultured bovine cells. By inserting the transgenic cell into an enucleated bovine egg, SCNT embryos were produced. The ability for SCNT embryos to develop to blastocysts was higher in hU6 based vector groups (44-53%) than in a tRNA group (32%). In all, 30 hU6-embryos and 12 tRNA-embryos were transferred to 11 recipients. Only tRNA-embryos were able to impregnate recipients (6 out of 11 transfers), resulting in four aborted fetuses, one stillbirth, and one live-born calf. The expression of EGFP, a marker, was detected in all six. The bPRNP transcript levels in the nervous tissues (brain, cerebellum, spinal bulb, and spinal cord) from the calf, which was killed 20 days after birth, were reduced to 35% of those of the control calf on average, as determined by qRT-PCR. The PrPC levels, as estimated by western blot were reduced to 86% on average in the nervous tissues. These findings suggest that SCNT technology remains immature, that the tRNA promoter is useful, and that RNAi can significantly reduce PRNP mRNA levels, but insufficient reduction of PrPC levels exists in cattle under these conditions.

  2. Production of biologically active recombinant bovine interferon-gamma by two different baculovirus gene expression systems using insect cells and silkworm larvae.

    Science.gov (United States)

    Murakami, K; Uchiyama, A; Kokuho, T; Mori, Y; Sentsui, H; Yada, T; Tanigawa, M; Kuwano, A; Nagaya, H; Ishiyama, S; Kaki, H; Yokomizo, Y; Inumaru, S

    2001-01-01

    The full-length bovine interferon-gamma (bIFN-gamma) cDNA, including the secretion signal peptide coding region was recloned into baculovirus transfer vectors pAcYM1 and pBm050. These vectors were co-transfected with Autographa californica nuclear polyhedrosis virus (AcNPV) or Bombyx mori nuclear polyhedrosis virus (BmNPV) DNA into Spodoptera frugiperda cells (SF21AE) and Bombyx mori cells (BmN), respectively. The recombinant viruses, named AcBIFN-gamma and BmBIFN-gamma, were then recovered. Recombinant bIFN-gamma (rbIFN-gamma) was accumulated in the culture fluid of AcBIFN-gamma-infected Trichoplusia ni cells and BmBIFN-gamma-infected silkworm larvae. These rbIFN-gamma forms were shown to be glycosylated 20 and 22 kDa proteins as confirmed by SDS-PAGE and tunicamycin treatment. These products were sensitive to cystein proteinase. Both rbIFN-gamma proteins, showed high-level biological activities by plaque reduction assay using vesicular stomatitis virus, and MHC class II antigen induction on bovine macrophage cells. PMID:11145838

  3. Bovine Tuberculosis, A Zoonotic Disease

    Directory of Open Access Journals (Sweden)

    Tarmudji

    2008-12-01

    Full Text Available Bovine tuberculosis is caused by the infection of Mycobacterium tuberculosis var. bovis (M. bovis. This species is one of Mycobacterium tuberculosis complex, can infect wide range of hosts: cattle and other domesticated animals, wild mammals and humans (zoonotic. M. bovis bacterium from infected hosts can be transmitted to other susceptible animals and humans through respiratory excretes and secretion materials. Humans can be infected with M. bovis by ingested M. bovis contaminated animal products, unpasteurised milk from tuberculosis cows or through respiratory route of contaminated aerosol. Bovine tuberculosis at the first stage does not show any clinical sign but as the disease progress in the next stage which may take several months or years, clinical signs may arise, suh as: fluctuative body temperature, anorexia, lost body weight, coughing, oedema of lymph nodes, increased respiratory frequencies. Pathological lesion of bovine tuberculosis is characterised by the formation of granulomas (tubercles, in which bacterial cells have been localised, most in lymph nodes and pulmonum, but can occur in other organs. The granulomas usually arise in small nodules or tubercles appear yellowish either caseus, caseo-calcareus or calcified. In Indonesia, bovine tuberculosis occurred in dairy cattle since 1905 through the imported dairy cows from Holland and Australian. It was unfortunate that until recently, there were not many research and surveilances of bovine tuberculosis conducted in this country, so the distribution of bovine tuberculosis is unknown. Early serological diagnosis can be done on live cattle by means of tuberculin tests under field conditions. Confirmation can be done by isolation and identification of excreted and secreted samples from the slaughter house. Antibiotic treatment and vaccination were uneffective, therefore the effective control of bovine tuberculosis is suggested by tuberculin tests and by slaughtering the selected

  4. Control of declared origin of bovine serum, a pilot study

    Science.gov (United States)

    Horacek, M.; Papesch, W.

    2009-04-01

    Bovine serum is the essential culture medium for cell cultures. Therefore it is highly demanded and the quality of the serum, e.g.: absence of bacteria, viruses certain antibodies, etc.., are important criteria. as some cattle diseases are endemic in certain regions, the origin of bovine serum is an important quality measure for its value. Thus the need to control the declared origins is present. Bovine serum was measured for d2H, d13C, d15N and d34S of proteine (dry residue) and d2H and d18O of the serum water. The hydrogen and oxygen are mainly depending by the isotopic composition of the water ingested by the cattle, and thus usually influenced by the isotopic signal of the precipitation. The carbon isotope signal is reflecting the diet of the cattle, whether it mainly feed on C3- or C4-plants. The nitrogen and sulphur isotope ratio is transferred from the ground/soil into the plant material and into the animal tissue, with some offset for nitrogen and without any significant offset for sulphur. Bovine serum samples from Canada, USA, Mexico, Brazil, Australia and New Zealand have been analysed. Due to the variations in the environmental conditions in different countries and regions which influence the isotope signatures of the serum samples it is possible to discriminate samples of different origin. Main discriminating parameters are d2H and d18O, d13C and d34S.

  5. Distribution and transfer of radiocesium and radioiodine in the environment following the Fukushima nuclear accident - Distribution and transfer of radiocesium and radioiodine in the environment of Fukushima Prefecture following the nuclear accident

    Energy Technology Data Exchange (ETDEWEB)

    Muramatsu, Yasuyuki; Ohno, Takeshi; Sugiyama, Midori [Gakushuin University, Toshima-ku, Tokyo, 171-8588 (Japan); Sato, Mamoru [Fukushima Agricultural Technology Centre, Koriyama, Fukushima 963-0531 (Japan); Matsuzaki, Hiroyuki [The University of Tokyo, Bunkyo-ku, Tokyo 113-8656 (Japan)

    2014-07-01

    Large quantities of radioiodine and radiocesium were released from the accident at the Fukushima Daiichi Nuclear Power Plant (FDNPP) in March 2011. We have carried out intensive studies on the distribution and behaviour of these nuclides in the environment following the accident. Two topics obtained from our studies are presented. (1) Retrospective estimation of I-131 deposition through the analysis of I-129 in soil: It is necessary to obtain deposition data of radioiodine in Fukushima Prefecture for the assessment of thyroid doses due to the accident. However, short half-life of I-131 (8 days) made it impossible to obtain adequate sample coverage that would permit direct determination of the regional deposition patterns of I-131 within the prefecture and surrounding areas. On the other hand, I-129 released simultaneously during the accident still remains in soil, due to its long half-life of 1.57x10{sup 7} years. In order to reconstruct the I-131 deposition, we have determined I-129 concentrations by accelerator mass spectrometry (AMS). A good correlation was found between the measured concentrations of I-131 and I-129 in soils collected in the vicinity of FDNPP. We have analyzed I-129 in more than 500 soil samples collected systematically from Fukushima Prefecture. Using the obtained results, the I-131 deposition was calculated in different areas and the deposition map for I-131 was constructed. We also studied the vertical distribution of I-129 in soil. (2) Peculiar accumulation of radiocesium to some plants and mushrooms The radioactivity levels in agricultural crops decreased markedly in some months following the accident and their concentrations became lower than the Japanese guideline for foodstuffs (500 Bq/kg in 2011, and 100 Bq/kg after 2012). However, some agricultural products such as tea leaves and citrus fruits showed relatively higher values. Our analytical results obtained for the distribution of radiocesium in tea trees show that the root uptake

  6. [Spectroscopic studies on the binding of phenazopyridine hydrochloride and bovine serum albumin].

    Science.gov (United States)

    Zhou, Hong; Chen, Chang-Yun; Xie, An-Jian

    2007-09-01

    The binding of phenazopyridine hydrochloride and bovine serum albumin under physiological conditions was studied by spectroscopic method. The quenching mechanism of the fluorescence of bovine serum albumin by phenazopyridine hydrochloride was studied with fluorescence and absorption spectroscopy. The binding constant Kb and the number of binding sites n were determined at different temperatures according to Scatchard equation, and the main binding force was discussed by thermodynamic equations. The effect of the drug on bovine serum albumin conformation was also studied by using synchronous fluorescence spectroscopy. The quenching mechanism of phenazopyridine hydrochloride to bovine serum albumin is static quenching and non-radiation energy transfer. The binding constants Kb at 15, 25 and 37 degrees C are 2.47 x 10(7), 9.15 x 10(6) and 4.36 x 10(6) mol(-1) with one binding site, respectively. The thermodynamic parameters of the reaction are DeltaH = -71.2 kJ x mol(-1), and DeltaS = 124.8 J x mol(-1) x K(-1). Binding phenazopyridine hydrochloride to bovine serum albumin is a spontaneous inter-molecular interaction in which entropy increases and Gibbs free energy decreases. The binding distance r between phenazopyridine hydrochloride and bovine serum albumin is 1.61 nm according to Forster theory of non-radiation energy transfer. The binding force is electrostatic interaction. Phenazopyridine hydrochloride can be deposited and transported by serum protein in vivo. Phenazopyridine hydrochloride does affect the serum protein conformation. PMID:18051539

  7. Testing nuclear forces by polarization transfer coefficients in d(p,p)d and d(p,d)p reactions at Ep=22.7 MeV

    Energy Technology Data Exchange (ETDEWEB)

    Evgeny Epelbaum

    2005-05-01

    The proton to proton polarization transfer coefficients K{sub x}{sup x'}, K{sub y}{sup y'}, K{sub z}{sup x'} and the proton to deuteron polarization transfer coefficients K{sub x}{sup x'}, K{sub y}{sup y'}, K{sub z}{sup x'}, K{sub x}{sup y'z'}, K{sub y}{sup z'z'}, K{sub z}{sup y'z'}, K{sub y}{sup x'z'} and K{sub y}{sup x'x'-y'y'} have been measured in d({rvec p}, {rvec p})d and d({rvec p}, {rvec d})p reactions at E{sub p}{sup lab} = 22.7 MeV, respectively. The data have been compared to predictions of modern nuclear forces obtained by solving the three-nucleon Faddeev equations in momentum space. Realistic (semi) phenomenological nucleon-nucleon potentials combined with model three-nucleon forces and modern chiral nuclear forces have been used. The AV18, CD Bonn, Nijm I and II nucleon-nucleon interactions have been applied alone or combined with the Tucson-Melbourne 99 three-nucleon force, adjusted separately for each potential to reproduce the triton binding energy. For the AV18 potential also the Urbana IX three-nucleon force have been used. In addition chiral NN potentials in the next-to-leading-order and chiral two- and three-nucleon forces in the next-to-next-to-leading-order have been applied. Only when three-nucleon forces are included a satisfactory description of all data results. For the chiral approach the restriction to the forces in the next-to-leading order is insufficient. Only when going over to the next-to-next-to-leading order one gets a satisfactory description of the data, similar to the one obtained with the (semi) phenomenological forces.

  8. Production of transgenic cashmere goat embryos expressing red fluorescent protein and containing IGF1 hair-follicle-cell specific expression cassette by somatic cell nuclear transfer

    Institute of Scientific and Technical Information of China (English)

    BOU; ShorGan

    2009-01-01

    In the present study, cashmere goat fetal fibroblasts were transfected with pCDsR-KI, a hair-follicle-cell specific expression vector for insulin-like growth factor 1 (IGF1) that contains two markers for selection (red fluorescent protein gene and neomycin resistant gene). The transgenic fibroblasts cell lines were obtained after G418 selection. Prior to the somatic cell nuclear transfer (SCNT), the maturation rate of caprine cumulus oocytes complexes (COCs) was optimized to an in vitro maturation time of 18 h. Parthenogenetic ooctyes were used as a model to investigate the effect of two activation methods, one with calcium ionophore IA23187 plus 6-DMAP and the other with ethanol plus 6-DMAP. The cleavage rates after 48 h were respectively 88.7% and 86.4%, with no significant difference (P>0.05). There was no significant difference between the cleavage rate and the blastocyst rate in two different media (SO- Faa and CR1aa; 86.3% vs 83.9%, P>0.05 and 23.1% vs 17.2%,P>0.05). The fusion rate of a 190 V/mm group (62.4%) was significantly higher than 130 V/mm (32.8%) and 200 V/mm (42.9%), groups (P<0.05). After transgenic somatic cell nuclear transfer (TSCNT) manipulation, 203 reconstructed embryos were obtained in which the cleavage rate after in vitro development (IVD) for 48 h was 79.3% (161/203). The blastocyst rate after IVD for 7 to 9 d was 15.3% (31/203). There were 17 embryos out of 31 strongly ex- pressing red fluorescence. Two of the red fluorescent blastocysts were randomly selected to identify transgene by polymerase chain reaction. Both were positive. These results showed that: (i) RFP and Neor genes were correctly expressed indicating that transgenic somatic cell lines and positive trans- genic embryos were obtained; (ii) one more selection at the blastocyst stage was necessary although the donor cells were transgenic positive, because only partially transgenic embryos expressing red fluorescence were obtained; and (iii) through TSCNT manipulation and

  9. Production of transgenic cashmere goat embryos expressing red fluorescent protein and containing IGF1 hair-follicle-cell specific expression cassette by somatic cell nuclear transfer

    Institute of Scientific and Technical Information of China (English)

    GUO XuDong; YANG DongShan; Ao XuDong; WU Xia; LI GuangPeng; WANG LingLing; BAO MingTao; XUE Lian; BOU ShorGan

    2009-01-01

    In the present study, cashmere goat fetal flbroblasta were transfected with pCDsR-KI, a hair-follicle-cell specific expression vector for insulin-like growth factor 1(IGF1) that contains two markers for selection (red fluorescent protein gene and neomycin resistant gene). The transgenic fibroblasta cell lines were obtained after G418 selection. Prior to the somatic cell nuclear transfer (SCNT), the maturation rate of caprine cumulus oocytes complexes (COCs) was optimized to an in vitro maturation time of 18 h.Parthenogenetic ooctyes were used as a model to Investigate the effect of two activation methods, one with calcium ionophore IA23187 plus 6-DMAP and the other with ethanol plus 6-DMAP. The cleavage rates after 48 h were respectively 88.7% and 86.4%, with no significant difference (P>0.05). There was no significant difference between the cleavage rate and the blastocyst rate in two different media (SO-Faa and CR1aa; 86.3% va 83.9%, P>0.05 and 23.1% vs 17.2%, P>0.05). The fusion rate of a 190 V/mm group (62.4%) was significantly higher than 130 V/mm (32.8%) and 200 V/mm (42.9%), groups (P<0.05).After transgenic somatic cell nuclear transfer (TSCNT) manipulation, 203 reconstructed embryos were obtained in which the cleavage rate after in vitro development (IVD) for 48 h was 79.3% (161/203). The blastocyst rate after IVD for 7 to 9 d was 15.3% (31/203). There were 17 embryos out of 31 strongly ex-pressing red fluorescence. Two of the red fluorescent blastocysta were randomly selected to identify transgene by polymeraee chain reaction. Both were positive. These results showed that: (i) RFP and Neo genes were correctly expressed indicating that transgenlc somatic cell lines and positive trans-genic embryos were obtained; (ii) one more selection at the blastocyst stage was necessary although the donor cells were transgenic positive, because only partially transgenic embryos expressing red fluorescence were obtained; and (iii) through TSCNT manipulation and

  10. A highly efficient method for generation of therapeutic quality human pluripotent stem cells by using naive induced pluripotent stem cells nucleus for nuclear transfer

    Directory of Open Access Journals (Sweden)

    Madhusudana Girija Sanal

    2014-09-01

    Full Text Available Even after several years since the discovery of human embryonic stem cells and induced pluripotent stem cells (iPSC, we are still unable to make any significant therapeutic benefits out of them such as cell therapy or generation of organs for transplantation. Recent success in somatic cell nuclear transfer (SCNT made it possible to generate diploid embryonic stem cells, which opens up the way to make high-quality pluripotent stem cells. However, the process is highly inefficient and hence expensive compared to the generation of iPSC. Even with the latest SCNT technology, we are not sure whether one can make therapeutic quality pluripotent stem cell from any patient’s somatic cells or by using oocytes from any donor. Combining iPSC technology with SCNT, that is, by using the nucleus of the candidate somatic cell which got reprogrammed to pluripotent state instead that of the unmodified nucleus of the candidate somatic cell, would boost the efficiency of the technique, and we would be able to generate therapeutic quality pluripotent stem cells. Induced pluripotent stem cell nuclear transfer (iPSCNT combines the efficiency of iPSC generation with the speed and natural reprogramming environment of SCNT. The new technique may be called iPSCNT. This technique could prove to have very revolutionary benefits for humankind. This could be useful in generating organs for transplantation for patients and for reproductive cloning, especially for childless men and women who cannot have children by any other techniques. When combined with advanced gene editing techniques (such as CRISPR-Cas system this technique might also prove useful to those who want to have healthy children but suffer from inherited diseases. The current code of ethics may be against reproductive cloning. However, this will change with time as it happened with most of the revolutionary scientific breakthroughs. After all, it is the right of every human to have healthy offspring and it is

  11. Transfer of 137Cs and 134Cs from litter into soil's of Japanese cypress forest after Fukushima nuclear accident in Karasawayama catchment, Tochigi prefecture

    Science.gov (United States)

    Mengistu, T. T.; Onda, Y.; Kato, H.; Gomi, T.

    2011-12-01

    The mega earthquake that rampaged north-east Japan on March 11, 2011 and the triggered subsequent tsunami hit the Fukushima Daiichi Nuclear power plant and resulted the discharge of about 770,000 terabecquerel radionuclide materials to the atmosphere. The distribution and deposition of the radionucides are then governed by the wind and rain following the release. When the cloud of radionuclide material by-pass the forest ecosystem (as radiodust-sinker), radionuclides are trapped and deposited to the forest floor through dry, litter, wet depositions. Litter-fall, as a key process of nutrient cycling in forest ecosystem, plays a great role in transferring canopy-trapped radionuclides to the forest soil. And so, we are monitoring the of Fukushima derived 137Cs deposition rate through litter to forest soil's of Japanese cypress(Chamaecyparis obutsa Sieb.et Zucc.) forests located approximately 160 km from the crippled nuclear power plant. For this purpose, five litter traps (1m2 areas each) were set up at one meter above the ground in the forest stand at the end of March 2011. Fukushima-derived 137Cs is then estimated from 134Cs:137Cs ratio as all 134Cs is originated from Fukushima. Within the two months of the accident, mean 134Cs:137Cs ratio was 0.8 in cypress litter. The inventories of both 137Cs and 134Cs in the upper 2cm forest soil were found 5089 Bq m-2 and 3571 Bq m-2, respectively. As a result, the amount of Fukushima-derived 137Cs deposition in upper 2cm soil layer by cypress litter is 4464 Bq m-2. This value account 88% of the total inventories of 137Cs in the upper 2cm soils and the other depositional paths (dry and wet fall) including old 137Cs cover only 12%. The transfer rate of 137Cs and 134Cs from canopy-litter to soil could be depend on litter's radionuclide adsorption strength (canopy and leaves architecture), the rate, amount of litter fall and its residence time in the canopy and forest floor. However, the results strongly confirmed that litter is

  12. Study of the nuclear spin-orbit interaction by performing the transfer reaction 36S(d,p)37S and 34Si(d,p)35Si

    International Nuclear Information System (INIS)

    The spin-orbit interaction depends on the spin orientation of the nucleons with respect to their angular momenta as well as on the derivative of the nuclear density. Even though this density dependence is used in all mean field model, it has never been tested yet due to the lack of data. We propose an original method to test this density dependence by comparing a bubble nucleus (34Si) to a normal nucleus (36S). The 34Si exhibits a central density which is depleted by a factor of two which induces a non-zero central density derivative and should change the strength of the spin orbit interaction for the inner orbits such as the p orbits (L=1). By performing (d,p) transfer reactions with 36S and 34Si beams, the p(3/2) and p(1/2) spin orbit splitting can be inferred for these nuclei. Depending on the models, the spin-orbit splitting varies from 7% (VlowK interaction) up to 70% (Relativistic mean field approach). Beams of 36S and 34Si, produced at the LISE spectrometer at 20 A.MeV, were impinged onto a CD2 target. Tracking the beam particles was achieved using 2 xy beam tracking gas detectors. Protons emitted were detected by 4 multi-segmented Si detectors (MUST2) placed at backwards angles. Gammas issued from the excited states decay were detected in the 4 EXOGAM segmented Germanium detectors. Transfer like nuclei were identified with an ionization chamber and a plastic detector. The excitation energy spectra of the 37S and 35Si are determined up to about 7 MeV. Spectroscopic factors and energies of p and f states are derived for the first time in 35Si. The two nuclei show strong similarity for the f spin-orbit partners, whereas the p(3/2) - p(1/2) energy gap is reduced by 55%. (author)

  13. Use of nuclear techniques for improving livestock production and health in Sri Lanka: A review of studies conducted and strategies for technology transfer

    International Nuclear Information System (INIS)

    The use of nuclear techniques for studies on livestock production in Sri Lanka commenced in the 1970's with the establishment of Radioimmunoassay(RIA) technique for measuring reproductive hormones in the blood and milk of buffaloes, cattle and goats. Progesterone measurement was used in a series of studies to monitor reproductive status of ruminants under small-holder farming conditions in different agro-ecological zones, to identify the major constraints and to test methods for improving fertility. Thereafter, other isotopic techniques were established and used together with conventional methods for studies on nutrition, environmental physiology and disease control. In the early 1980's the nuclear-related technique of Enzyme-linked Immunosorbent Assay (ELISA) was established and applied for studies on the immune response of buffaloes to Toxocara vitulorum infection. Subsequently, ELISA techniques were used for studies on sero-epidomology and control of important viral and bacterial disease of cattle and buffaloes (rotavirus infection, haemorrhagic specticaemia, brucellosis, rinderpest and foot-and-mouth disease). The most recent development has been the use of ELISA for diagnosing viral diseases of poultry. In order to transfer the findings from research to the end-users, a multi disciplinary programme was launched in 1995, with the focus on improving buffalo production. Selected farms in three regions of the country participated in the testing, modification and evaluation of appropriate technology packages aimed at imroving the productivity and health of their animals in a sustainable and economically feasible manner. They were provided assistance to upgrade their operations to the status of farms, which are now serving as demonstration sites and training locations for other farmers (AU)

  14. Bovine respiratory disease model based on dual infections with infection with bovine viral diarrhea virus and bovine corona virus

    Science.gov (United States)

    Bovine respiratory disease complex (BRDC) is the leading cause of economic loss in the U.S. cattle industry. BRDC likely results from simultaneous or sequential infections with multiple pathogens including both viruses and bacteria. Bovine viral diarrhea virus (BVDV) and bovine corona virus (BoCV...

  15. Direct determination of tellurium in soil and plant samples by sector-field ICP-MS for the study of soil-plant transfer of radioactive tellurium subsequent to the Fukushima Daiichi Nuclear Power Plant accident

    International Nuclear Information System (INIS)

    The Fukushima Daiichi Nuclear Power Plant (FDNPP) accident caused the release of large amounts of radioactive Te into the environment. Stable Te, as an analogue, is considered to be useful for the estimation of the soil-plant transfer of radioactive Te. It is necessary to estimate the radiation dose of Te that would result from food ingestion. However, due to the extremely low concentrations of Te in the environment, reported transfer factor values for Te are considerably limited. We report a sensitive analytical method for direct determination of trace Te in soil and plant samples using a sector-field inductively coupled plasma mass spectrometry (SF-ICP-MS). The developed analytical method is characterized by a very low detection limit at the sub-parts per billion (ng g-1) level in soil and plant samples, and it has been applied to the study of soil-plant transfer to collect transfer factor data in Japan. (author)

  16. Association of Bovine Viral Diarrhea Virus with Multiple Viral Infections in Bovine Respiratory Disease Outbreaks

    OpenAIRE

    Richer, Lisette; Marois, Paul; Lamontagne, Lucie

    1988-01-01

    We investigated eleven outbreaks of naturally occurring bovine respiratory diseases in calves and adult animals in the St-Hyacinthe area of Quebec. Specific antibodies to bovine herpesvirus-1, bovine viral diarrhea virus, respiratory syncytial virus, parainfluenza type 3 virus, reovirus type 3, and serotypes 1 to 7 of bovine adenovirus were found in paired sera from diseased animals. Several bovine viruses with respiratory tropism were involved concomitantly in herds during an outbreak of bov...

  17. Generation of liver-specific TGF-α/c-Myc-overexpressing porcine induced pluripotent stem-like cells and blastocyst formation using nuclear transfer.

    Science.gov (United States)

    Park, Kyung-Mee; Lee, Joohyeong; Hussein, Kamal Hany; Hong, Seok-Ho; Yang, Se-Ran; Lee, Eunsong; Woo, Heung-Myong

    2016-05-01

    Transgenic porcine induced pluripotent stem (iPS) cells are attractive cell sources for the development of genetically engineered pig models, because they can be expanded without senescence and have the potential for multiple gene manipulation. They are also useful cell sources for disease modeling and treatment. However, the generation of transgenic porcine iPS cells is rare, and their embryonic development after nuclear transfer (NT) has not yet been reported. We report here the generation of liver-specific oncogenes (TGF-α/c-Myc)-overexpressing porcine iPS (T/M iPS)-like cells. They expressed stem cell characteristics and were differentiated into hepatocyte-like cells that express oncogenes. We also confirmed that NT embryos derived from T/M iPS-like cells successfully developed blastocysts in vitro. As an initial approach toward porcine transgenic iPS cell generation and their developmental competence after NT, this study provides foundations for the efficient generation of genetically modified porcine iPS cells and animal models. PMID:26725870

  18. Characterisation and application of a bovine U6 promoter for expression of short hairpin RNAs

    Directory of Open Access Journals (Sweden)

    Dalrymple Brian P

    2005-05-01

    Full Text Available Abstract Background The use of small interfering RNA (siRNA molecules in animals to achieve double-stranded RNA-mediated interference (RNAi has recently emerged as a powerful method of sequence-specific gene knockdown. As DNA-based expression of short hairpin RNA (shRNA for RNAi may offer some advantages over chemical and in vitro synthesised siRNA, a number of vectors for expression of shRNA have been developed. These often feature polymerase III (pol. III promoters of either mouse or human origin. Results To develop a shRNA expression vector specifically for bovine RNAi applications, we identified and characterised a novel bovine U6 small nuclear RNA (snRNA promoter from bovine sequence data. This promoter is the putative bovine homologue of the human U6-8 snRNA promoter, and features a number of functional sequence elements that are characteristic of these types of pol. III promoters. A PCR based cloning strategy was used to incorporate this promoter sequence into plasmid vectors along with shRNA sequences for RNAi. The promoter was then used to express shRNAs, which resulted in the efficient knockdown of an exogenous reporter gene and an endogenous bovine gene. Conclusion We have mined data from the bovine genome sequencing project to identify a functional bovine U6 promoter and used the promoter sequence to construct a shRNA expression vector. The use of this native bovine promoter in shRNA expression is an important component of our future development of RNAi therapeutic and transgenic applications in bovine species.

  19. Production of transgenic embryos through nuclear transfer using ovine fetal fibroblasts transferred with foreign genes%体细胞核移植生产绵羊转hALR基因囊胚

    Institute of Scientific and Technical Information of China (English)

    马玉珍; 任宇; 周雪原; 刘东军; 旭日干

    2011-01-01

    扩增人肝细胞再生增强因子(human augmenter of liver regeneration,ALR)基因,利用质粒pIRES2-EGFP 构建新霉素(Neo)、增强绿色荧光蛋白(enhanced green fluorescence protein,EGFP)双标记基因且EGFP和ALR基因为双顺反子的真核表达载体.LipofectAMINETM介导其转染体外培养的绵羊胎儿成纤维细胞(sheep fetal fibroblast cells,sFFCs);经G418筛选转基因细胞;激光共聚焦显微镜挑选绿色荧光单克隆细胞.PCR、RT-PCR和免疫组织化学方法进一步检测ALR基因及其表达;稳定表达外源基因的sFFCs作供体,移入去核的绵羊卵母细胞中,进行体细胞核移植.通过激光共聚焦显微镜和ALR抗体检测EGFP、ALR基因在胚胎水平上的表达,其结果表明:由IRES连接的EGFP和ALR基因可在绵羊胎儿成纤维细胞内同时表达,由此细胞核移植产生的转基因胚胎在发育的各阶段均可见绿色荧光;囊胚中所有细胞表达EGFP基因;发绿色荧光的胚胎中ALR基因同时存在.因此,由IRES连接标记基因和目的基因,以标记基因指示目的基因的表达,可简化检测目的基因的繁琐手段;用筛选的转基因早期胚胎进行移植,可提高制备转基因动物的效率.%Human ALR gene sequence was amplified by PCR from human total DNA and inserted into PIRES2-EGFP vector. The bicistronic eukaryotic expression vector, pIRES-EGFP/ALR, expressing EGFP, Neo and ALR genes was constructed. Sheep fetal fibroblast cells (sEFCs) were transfected with pIRES-EGFP/AZJ? By the induction of lipofectAMINE?. The positive cell clones were selected with medium containing G418 (800 ug/mL). The fluorescence of transgenic cells was examined with a confocal laser scanning microscope. The expression of ALR gene was tested by PCR, RT-PCR and immuno-histochemical staining. The transgenic cells were used as donors for nuclear transfer to enucleated ovine oocytes. Transgenic embryos were tested by confocal laser scanning microscope and

  20. Propionate induces the bovine cytosolic phosphoenolpyruvate carboxykinase promoter activity.

    Science.gov (United States)

    Zhang, Qian; Koser, Stephanie L; Donkin, Shawn S

    2016-08-01

    Cytosolic phosphoenolpyruvate carboxykinase (PCK1) is a critical enzyme within the metabolic networks for gluconeogenesis, hepatic energy metabolism, and tricarboxylic acid cycle function, and is controlled by several transcription factors including hepatic nuclear factor 4α (HNF4α). The primary objective of the present study was to determine whether propionate regulates bovine PCK1 transcription. The second objective was to determine the action of cyclic AMP (cAMP), glucocorticoids, and insulin, hormonal cues known to modulate glucose metabolism, on bovine PCK1 transcriptional activity. The proximal promoter of the bovine PCK1 gene was ligated to a Firefly luciferase reporter and transfected into H4IIE hepatoma cells. Cells were exposed to treatments for 23 h and luciferase activity was determined in cell lysates. Activity of the PCK1 promoter was linearly induced by propionate, and maximally increased 7-fold with 2.5 mM propionate, which was not muted by 100 nM insulin. Activity of the PCK1 promoter was increased 1-fold by either 1.0 mM cAMP or 5.0µM dexamethasone, and 2.2-fold by their combination. Induction by cAMP and dexamethasone was repressed 50% by 100 nM insulin. Propionate, cAMP, and dexamethasone acted synergistically to induce the PCK1 promoter activity. Propionate-responsive regions, identified by 5' deletion analysis, were located between -1,238 and -409 bp and between -85 and +221 bp. Deletions of the core sequences of the 2 putative HNF4α sites decreased the responsiveness to propionate by approximately 40%. These data indicate that propionate regulates its own metabolism through transcriptional stimulation of the bovine PCK1 gene. This induction is mediated, in part, by the 2 putative HNF4α binding sites in the bovine PCK1 promoter. PMID:27289145

  1. Material Properties of Inorganic Bovine Cancellous Bovine: Nukbone

    Science.gov (United States)

    Piña, Cristina; Palma, Benito; Munguía, Nadia

    2006-09-01

    In this work, inorganic cancellous bovine bone implants prepared in the Instituto de Investigaciones en Materiales — UNAM were characterized. Elementary chemical analysis was made, toxic elements concentration were measured and the content of organic matter also. These implants fulfill all the requirements of the ASTM standards, and therefore it is possible their use in medical applications.

  2. 4 years of successful knowledge transfer - the nuclear technology training center of the TUeV Nord Group; 4 Jahre erfolgreicher Wissenstransfer - das Ausbildungszentrum fuer Kerntechnik der TUeV Nord Gruppe

    Energy Technology Data Exchange (ETDEWEB)

    Willenbockel, I. [TUeV NORD EnSys Hannover GmbH und Co. KG, Abt. ETE, Hannover (Germany); Tietze, U. [TUeV NORD SysTec, Ausbildungszentrum fuer Kerntechnik, Hamburg (Germany)

    2007-02-15

    In connection with the 2002 amendment to the German Atomic Energy Act, the topics of generational change and maintenance of competence grew in importance and necessitated new solution approaches. To this end, various activities were launched, with the aim of conducting conceptual analyses of these topics. Examples include the 'National Competence Network for Nuclear Technology' (Nationaler Kompetenzverbund fuer Kerntechnik), various networks established by colleges and universities, the 'Knowledge Management for the Maintenance and Transfer of Competence in Reactor Safety' (Wissensmanagement zum Kompetenzerhalt und -transfer in der Reaktorsicherheit) workshop held in 2001 in Garching near Munich (Germany) and the 'Ad-hoc Workgroup on the Maintenance of Competence' (Ad-hoc-Arbeitskreis Kompetenzerhalt) of the VdTUeV. The nuclear technology departments of the TUeV Nord Group were aware of te challenges associated with the generational change early on. By establishing the 'Nuclear Technology Training Center' (Ausbildungszentrum fuer Kerntechnik, AfK), the TUeV Nord Group intended to ensure the required knowledge transfer during the generational change as well as maintain the renowned high qualification as regards the subject of nuclear technology and thus continue to provide - in the sense of social responsibility - crucial contribution to the long-term safety of nuclear plants. Four years have passed since the training center held the first courses in the fall of 2002. Up to now, more than 350 participants have been trained in the courses conducted by the AfK. In the opinion of the TUeV Nord Group, the activities of the AfK have laid the foundation for a successful change of generations within the group's nuclear technology organizations. (orig.)

  3. Comparative Genomic Analysis of the ICESa2603 Family ICEs and Spread of erm(B)- and tet(O)-Carrying Transferable 89K-Subtype ICEs in Swine and Bovine Isolates in China.

    Science.gov (United States)

    Huang, Jinhu; Liang, Yuan; Guo, Dawei; Shang, Kexin; Ge, Lin; Kashif, Jam; Wang, Liping

    2016-01-01

    Integrative and conjugative elements (ICEs) of the ICESa2603 family have been isolated from several species of Streptococcus spp.; however, the comparative genomic and evolutionary analyses of these particular ICEs are currently only at their initial stages. By investigating 13 ICEs of the ICESa2603 family and two ICESa2603 family-like ICEs derived from diverse hosts and locations, we have determined that ICEs comprised a backbone of 30 identical syntenic core genes and accessory genes that were restricted to the intergenic sites or the 3'-end of the non-conserved domain of core genes to maintain its function. ICESa2603 family integrase IntICE Sa 2603 specifically recognized a 15-bp att sequence (TTATTTAAGAGTAAC) at the 3'-end of rplL, which was highly conserved in genus Streptococcus. Phylogenetic analyses suggest that extensive recombination/insertion and the occurrence of a hybrid/mosaic in the ICESa2603 family were responsible for the significant increase in ICE diversity, thereby broadening its host range. Approximately 42.5 and 38.1% of the tested Streptococcus suis and Streptococcus agalactiae clinical isolates respectively contained ICESa2603 family Type IV secretion system (T4SS) genes, and 80.5 and 62.5% of which also respectively carried int ICE Sa 2603, indicating that ICESa2603 family is widely distributed across these bacteria. Sequencing and conjugation transfer of a novel sequence type ST303 clinical S. suis isolate HB1011 demonstrated that the 89K-subtype ICESsuHB1011 retained its transferrable function, thereby conferring tetracycline and macrolide resistance. PMID:26870017

  4. Comparative Genomic Analysis of the ICESa2603 Family ICEs and Spread of erm(B- and tet(O-Carrying Transferable 89K-Subtype ICEs in Swine and Bovine Isolates in China

    Directory of Open Access Journals (Sweden)

    Jinhu eHuang

    2016-02-01

    Full Text Available Integrative and conjugative elements (ICEs of the ICESa2603 family have been isolated from several species of Streptococcus spp.; however, the comparative genomic and evolutionary analyses of these particular ICEs are currently only at their initial stages. By investigating 13 ICEs of the ICESa2603 family and two ICESa2603 family-like ICEs derived from diverse hosts and locations, we have determined that ICEs comprised a backbone of 30 identical syntenic core genes and accessory genes that were restricted to the intergenic sites or the 3'-end of the non-conserved domain of core genes to maintain its function. ICESa2603 family integrase IntICESa2603 specifically recognized a 15-bp att sequence (TTATTTAAGAGTAAC at the 3'-end of rplL, which was highly conserved in genus Streptococcus. Phylogenetic analyses suggest that extensive recombination/insertion and the occurrence of a hybrid/mosaic in the ICESa2603 family were responsible for the significant increase in ICE diversity, thereby broadening its host range. Approximately 42.5% and 38.1% of the tested Streptococcus suis and Streptococcus agalactiae clinical isolates respectively contained ICESa2603 family Type Ⅳ secretion system (T4SS genes, and 80.5% and 62.5% of which also respectively carried intICESa2603, indicating that ICESa2603 family is widely distributed across these bacteria. Sequencing and conjugation transfer of a novel sequence type ST303 clinical S. suis isolate HB1011 demonstrated that the 89K-subtype ICESsuHB1011 retained its transferrable function, thereby conferring tetracycline and macrolide resistance.

  5. Indução do parto de bovinos aos 270 dias de gestação com a utilização prévia de glicocorticoide de longa ação e a transferência de imunidade passiva = Inducing bovine parturition at 270 pregnancy days with early use of a long-acting glucocorticoid and passive immunity transfer

    Directory of Open Access Journals (Sweden)

    Alfredo Quites Antoniazzi

    2009-01-01

    Full Text Available O trabalho teve por objetivo avaliar a taxa de transferência de imunidade passiva e de retenção de placenta com a utilização prévia de um glicocorticoide de longa ação na indução do parto bovino aos 270 dias de gestação, bem como verificar a eficácia dos protocolos. Foram utilizadas 42 vacas prenhes, de corte e de leite, leatoriamente separadas em três grupos: Grupo-controle (C que não recebeu aplicação hormonal, mas o mesmo manejo; Grupo longa ação + indução (LA que recebeu 30 mg de acetato de metilprednisolona, via subcutânea, no dia 255 de gestação e 20 mg de dexametasona associada a 0,5 mg de cloprostenol sódico, via intramuscular, no dia 270 de gestação; e Grupo indução (I que recebeu 20 mg de dexametasona associada a 0,5 mg de cloprostenol sódico no dia 270 de gestação. A utilização prévia do glicocorticoide de longa ação não mostrou diferença na taxa de transferência de imunidade passiva e nos índices de retenção de placenta. As fêmeas dos Grupos LA e I pariram em 41,36 ± 1,89h após a aplicação hormonal de dexametasona associada ao cloprostenol, independente de aplicação prévia de glicocorticoide de longa ação no Grupo LA. A indução da parição com dexametasonaassociada ao cloprostenol, independente da aplicação prévia de acetato de metilprednisolona, mostrou-se eficaz em todos dos animais.This experiment had the objective of evaluating passive immunity transfer and placental retention rates with the early use of a long-acting glucocorticoid to induce bovine parturition at 270 pregnancy days, as well as comparing protocol efficacy regarding the timing of parturition induction. Fortytwo pregnant cows were selected from a beef and dairy herd and randomly assigned into one of three groups: control (C without drug injection, but with the same management; long-acting + induction group (LA, with one injection of 30 mg prednisolone methylacetate, subcutaneous, on pregnancy day 255 and

  6. Superovulatory response and embryonic progressive in Iranian Qezel ewes treated with two different concentrations of bovine somatotropin

    Directory of Open Access Journals (Sweden)

    Amir Hossein Asgari Safdar

    2016-05-01

    Conclusions: The treatment 50 mg bovine somatotropins enhance the ratio and growth of the transferable embryos. Embryos of bST-50 treatment indicated an improved embryonic development but bST did not affect the pregnancy rates of transferred embryos.

  7. Experimental investigations of heat transfer and temperature fields in models simulating fuel assemblies used in the core of a nuclear reactor with a liquid heavy-metal coolant

    Science.gov (United States)

    Belyaev, I. A.; Genin, L. G.; Krylov, S. G.; Novikov, A. O.; Razuvanov, N. G.; Sviridov, V. G.

    2015-09-01

    The aim of this experimental investigation is to obtain information on the temperature fields and heat transfer coefficients during flow of liquid-metal coolant in models simulating an elementary cell in the core of a liquid heavy metal cooled fast-neutron reactor. Two design versions for spacing fuel rods in the reactor core were considered. In the first version, the fuel rods were spaced apart from one another using helical wire wound on the fuel rod external surface, and in the second version spacer grids were used for the same purpose. The experiments were carried out on the mercury loop available at the Moscow Power Engineering Institute National Research University's Chair of Engineering Thermal Physics. Two experimental sections simulating an elementary cell for each of the fuel rod spacing versions were fabricated. The temperature fields were investigated using a dedicated hinged probe that allows temperature to be measured at any point of the studied channel cross section. The heat-transfer coefficients were determined using the wall temperature values obtained at the moment when the probe thermocouple tail end touched the channel wall. Such method of determining the wall temperature makes it possible to alleviate errors that are unavoidable in case of measuring the wall temperature using thermocouples placed in slots milled in the wall. In carrying out the experiments, an automated system of scientific research was applied, which allows a large body of data to be obtained within a short period of time. The experimental investigations in the first test section were carried out at Re = 8700, and in the second one, at five values of Reynolds number. Information about temperature fields was obtained by statistically processing the array of sampled probe thermocouple indications at 300 points in the experimental channel cross section. Reach material has been obtained for verifying the codes used for calculating velocity and temperature fields in channels with

  8. Identification of Valid Housekeeping Genes for Real-Time Quantitative PCR Analysis of Collapsed Lung Tissues of Neonatal Somatic Cell Nuclear Transfer-Derived Cattle.

    Science.gov (United States)

    Liu, Yan; Zhang, Yunhai; Jiang, Qiuling; Rao, Man; Sheng, Zheya; Zhang, Yu; Du, Weihua; Hao, Haisheng; Zhao, Xueming; Xu, Zhe; Liu, Jianning; Zhu, Huabin

    2015-10-01

    Cloned calves produced by somatic cell nuclear transfer frequently suffer alveolar collapse as newborns. To study the underlying pathophysiological mechanisms responsible for this phenomenon, the expression profiles of numerous genes involved in lung development need to be investigated. Quantitative real-time PCR is commonly adopted in gene expression analysis. However, selection of an appropriate reference gene for normalization is critical for obtaining reliable and accurate results. Seven housekeeping genes-β-glucuronidase (GUSB), phosphoglycerate kinase 1 (PGK1), β-2-microglobolin (B2M), peptidylprolyl isomerase A (PPIA), glyceraldehyde-3-phosphate dehydrogenase (GAPDH), TATA-box binding protein (TBP), and 5.8S ribosomal RNA (5.8S rRNA)-were selected and evaluated as candidates. Their gene expression levels in the collapsed lungs of deceased neonate cloned calves and normal lung derived from normal calves were assessed. The ranking of gene expression stability was estimated by the geNorm, NormFinder, and BestKeeper programs. 5.8S rRNA and PPIA were determined to be the most stable reference genes by geNorm and BestKeeper, whereas the combination of GAPDH and TBP was suggested as reference genes by NormFinder. Taking these results into account, we conclude that 5.8S rRNA and PPIA could be the most reliable reference genes for studying the genes involved in alveolar collapse. Moreover, 5.8S rRNA could be represented as a uniform reference gene in similar cases. PMID:26393896

  9. Effects of the reproductive status on morphological oocyte quality and developmental competence of oocytes after in vitro fertilization and somatic cell nuclear transfer in cat.

    Science.gov (United States)

    Naoi, H; Agung, B; Karja, N W K; Wongsrikeao, P; Shimizu, R; Taniguchi, M; Otoi, T

    2008-04-01

    This study was conducted to examine the effects of the reproductive cycle of donor cat on the quality of oocytes at recovery and developmental competence of oocytes after in vitro fertilization (IVF) and somatic cell nuclear transfer (NT). Based on the presence or absence of follicles and corpora lutea, the ovarian pairs collected were classified into inactive, follicular or luteal stages. After collection of oocytes, the oocytes were classified into four grades according to the morphological condition of oocyte cytoplasm and cumulus cells. A total of 16 558 oocytes were obtained from 198 ovarian pairs. The total mean numbers of oocytes and the mean numbers of oocytes with high quality (grade I) were significantly higher in ovarian pairs at the inactive stage (111.1 and 19.0 oocytes, respectively) than in ovarian pairs at the follicular stage (67.1 and 11.4 oocytes, respectively). A significant difference in the proportions of oocytes with grade I out of the total examined oocytes was observed between the follicular and luteal stages of ovaries (14.9% vs 20.2%, p quality of oocytes at recovery, irrespective of the reproductive status of ovaries. Moreover, there were no significant differences in the proportions of cleavage and development to the blastocyst stage of IVF and NT embryos among three oestrous stages of ovaries. These results indicate that the reproductive cycle stage of donor cat ovaries has no apparent effects on the in vitro development of oocytes after IVF and NT, but the quality of oocytes at recovery influences the development of IVF embryos. PMID:18325005

  10. Tumorigenesis of nuclear transfer-derived embryonic stem cells is reduced through differentiation and enrichment following transplantation in the infarcted rat heart.

    Science.gov (United States)

    Fu, Qiang; Su, Dechun; Wang, Ke; Zhao, Yingjun

    2016-06-01

    The aim of the present study was to evaluate the tumorigenic potential of nuclear transfer-derived (nt) mouse embryonic stem cells (mESCs) transplanted into infarcted rat hearts. The nt‑mESCs were cultured using a bioreactor system to develop embryoid bodies, which were induced with 1% ascorbic acid to differentiate into cardiomyocytes. The nt‑mESC‑derived cardiomyocytes (nt‑mESCs‑CMs) were enriched using Percoll density gradient separation to generate nt‑mESCs‑percoll‑enriched (PE)‑CMs. Ischemia was induced by ligating the left anterior descending coronary artery in female Sprague‑Dawley rats. Immunosuppressed rats (daily intraperitoneal injections of cyclosporine A and methylprednisolone) were randomly assigned to receive an injection containing 5x106 mESCs, nt‑mESCs, nt‑mESC‑CMs or nt‑mESC‑PE‑CMs. Analysis performed 8 weeks following transplantation revealed teratoma formation in 80, 86.67 and 33.33% of the rats administered with the mESCs, nt‑mESCs and nt‑mESC‑CMs, respectively, indicating no significant difference between the mESCs and nt‑mESCs; but significance (P0.05 mESCs, vs. nt‑mESCs; P<0.05 nt‑mESC‑CMs, vs. nt‑mESCs). By contrast, no teratoma formation was detected in the rats, which received nt‑mESC‑PE‑CMs. Octamer‑binding transcription factor‑4, a specific marker of undifferentiated mESCs, was detected using polymerase chain reaction in the rats, which received nt‑mESCs and nt‑mESC‑CMs, but not in rats administered with nt‑mESC‑PE‑CMs. In conclusion, nt‑mESCs exhibited the same pluripotency as mESCs, and teratoma formation following nt‑mESC transplantation was reduced by cell differentiation and enrichment.

  11. Developmental Outcome and Related Abnormalities in Goats: Comparison Between Somatic Cell Nuclear Transfer- and In Vivo-Derived Concepti During Pregnancy Through Term.

    Science.gov (United States)

    Martins, Leonardo Tondello; Neto, Saul Gaudêncio; Tavares, Kaio César Simiano; Calderón, Carlos Enrique Méndez; Aguiar, Luis Henrique; Lazzarotto, Cícera Regina; Ongaratto, Felipe Ledur; Rodrigues, Victor Hugo Vieira; Carneiro, Igor de Sá; Rossetto, Rafael; Almeida, Anderson Pinto; Fernandes, César Carneiro Linhares; Rondina, Davide; Dias, Ana Christina Oliveira; Chies, Jocelei Maria; Polejaeva, Irina A; Rodrigues, José Luiz; Forell, Fabiana; Bertolini, Luciana Relly; Bertolini, Marcelo

    2016-08-01

    Cloning by somatic cell nuclear transfer (SCNT) is characterized by low efficiency and the occurrence of developmental abnormalities, which are rather poorly studied phenomena in goats. This study aimed at comparing overall SCNT efficiency in goats by using in vitro-matured (IVM) or in vivo-matured oocytes and fibroblast donor cells (mock transfected, transgenic, or wild type), also characterizing symptoms of the Abnormal Offspring Syndrome (AOS) in development, comparing results with pregnancies produced by artificial insemination (AI) and in vivo-derived (IVD) embryos. The SCNT group had lower pregnancy rate (18.3%, 11/60), total number of concepti (20.0%, 12/60), term births (3.3%, 2/60), and live births (1.7%, 1/60) than both the IVD (77.8%, 7/9; 155.5%, 14/9; 122.2%, 11/9; 88.8%, 8/9) and the AI (71.4%, 10/14; 121.4%, 17/14; 100%, 14/14; 78.5%, 11/14) groups, respectively (p < 0.05). No SCNT pregnancies reached term using IVM oocytes, but in vivo-matured oocytes resulted in two term transgenic cloned kids. The proportion fetal membrane (FM) weight/birth weight reflected an increase in FM size and cotyledonary enlargement in clones, for disproportionally bigger newborns in relation to cotyledonary numbers. Overall, goat cloning showed losses and abnormality patterns similar to the AOS in cloned cattle and sheep, which have not been previously well recognized in goats. PMID:27362734

  12. Comparison of the efficacy of conventional slow freezing and rapid cryopreservation methods for bovine embryos

    NARCIS (Netherlands)

    Wagtendonk-de Leeuw, van A.M.; den Daas, J.H.; Kruip, T.A.; Rail, W.F.

    1995-01-01

    Day 7 bovine morulae and early blastocysts were randomly assigned to one of four cryopreservation methods: (i) a modified conventional controlled slow freezing and stepwise dilution after thawing; and three methods which enable direct transfer of the embryo into the recipient upon thawing: (ii) conv

  13. 一例体细胞克隆水牛脂肪及皮肤的组织结构观察%Histological Structure Observation of the Fat and Skin in Somatic Nuclear Transfered Buffalo

    Institute of Scientific and Technical Information of China (English)

    王晓丽; 周石琼; 李波; 魏晓瑞; 崔奎青; 石德顺

    2012-01-01

    应用光镜及HE染色等技术对体细胞克隆水牛的脂肪和皮肤组织及其细胞类型和形态进行了详细的观察,旨在为以后体细胞克隆牛的研究提供一定的理论依据,结果表明,脂肪组织外周有疏松结缔组织包裹,脂肪小叶不明显;皮肤由表皮和真皮构成,借助皮下组织与深部的肌肉相连,局部表皮有脱落,皮肤土有毛囊分布.%To provide a certain basis for the study of somatic nuclear transfer buffalo, the histological structure and its cell morphology of adipose and skin tissue in somatic nuclear transferred buffalo were observed by anatomy, HE staining techniques. The results showed that the adipose tissue of somatic nuclear transfer buffalo were encapsulated by loose connective tissue, and the fat lobule was not obvious; The skin was composed of epidermis and dermis, and connected with muscle by hypodermis. Part of epidermis was abscission. There were hair follicles.

  14. Insights on bovine genetic engineering and cloning

    OpenAIRE

    Fabiana F. Bressan; Sangalli, Juliano R.; Laís V.F. Pessôa; Pedro R.L. Pires; Flávio V. Meirelles

    2013-01-01

    Transgenic technology has become an essential tool for the development of animal biotechnologies, and animal cloning through somatic cell nuclear transfer (SCNT) enabled the generation of genetically modified animals utilizing previously modified and selected cell lineages as nuclei donors, assuring therefore the generation of homogeneous herds expressing the desired modification. The present study aimed to discuss the use of SCNT as an important methodology for the production of transgenic h...

  15. Human metabolism and ecological transfer of radioactive caesium: Comparative studies of Chernobyl debris and nuclear weapons fallout, in Southern Sweden and in Bryansk, Russia

    Science.gov (United States)

    Raaf, Christopher Leopold

    The whole-body content of radiocaesium was measured in a South-Swedish urban group of people residing in the city of Lund (55.7°N, 13.2°E) between 1960 and 1994. The results from the survey have been analysed in order to estimate the ecological half-time, Teff,eco, of fallout radiocaesium and the aggregate transfer from ground deposition to man in the region. After 1987, the biological half-times, Ts, of 137Cs and 40K in man were also determined in the reference group through whole-body content measurements in combination with 24-hour urine sampling. Relationships between 24-hour urinary excretion and body burden of 137Cs in the group together with data from the literature were then applied to urine samples collected in 1994 and 1995 from adult subjects living in the highly contaminated region of Bryansk, Russia, in order to estimate their average body burden of 137Cs. The equivalent biological half-time for 137Cs in females of the Lund reference group was, on average (+/-1 WSE), 66 +/- 3 d, which agrees with other findings, whereas the value for the males, 81 +/- 4 d, was, on average, significantly lower than what is found in the literature. This is partly explained by the elevated mean age and relatively low mean body muscle mass of the males investigated in the group during the post-Chernobyl study period. The effective ecological half-time for 137Cs from Chernobyl was found to be 1.8 +/- 0.2 y. The aggregate transfer of 137Cs from deposition to mean activity concentration in man was estimated to be 1.7 Bq kg-1/kBq m-2. These vales may be compared with an effective ecological half-time of 1.3 years found in the reference group in the 1960s, and an aggregate transfer factor of 9.8 Bq kg-1/kBq m-2. The average committed effective dose from ingested 137Cs Chernobyl fallout in the study group was estimated to be 0.02 mSv and from the nuclear weapons fallout to 0.20 mSv. The estimates of whole-body content of 137Cs in the Russian subjects obtained through

  16. Transfer of financial obligations for the disposal of nuclear waste and decommissioning of German NPP's. Legal aspects of a trust model; Sicherstellung der finanziellen Entsorgungsvorsorge fuer die Stilllegungs- und Rueckbaukosten der deutschen Kernkraftwerke. Rechtliche Randbedingungen eines Stiftungsmodells

    Energy Technology Data Exchange (ETDEWEB)

    Schewe, Markus; Wiesendahl, Stefan [Kuemmerlein Rechtsanwaelte und Notare, Essen (Germany)

    2015-04-15

    The nuclear power plant operators have to bear the costs associated with the closure and the decommissioning of the German nuclear power plants as well as the costs for the disposal of nuclear waste. For that purpose, the operators have to build up sufficient reserves for the decommissioning phase. These reserves at the end of 2013 amounted to approximately 36 billion Euro. Changing this system is discussed very so often. Last in May 2014, a public debate started dealing with the so called trust model (''Stiftungsmodell''). The press published deliberations of several operators to transfer their entire nuclear business to the Federal Republic of Germany. Under this deliberation the current nuclear power plant operations, as well as closure obligations would be contributed to trust. Further, also the reserves should be ''transferred'' to the trust. RAG-Foundation (RAG-Stiftung) - which will assume the financial obligations in connection with Germany's closure of underground coal mining activities - sometimes is cited as a role model. The article covers elements of German trust law and atomic energy law regarding such deliberations. In trust law e.g. it can be debated whether the trust should be established under public or - as in the case of RAG-Foundation - under private law. In this context we will set out the major differences between those two options. In the public law part we will notably address issues arising from individual licensing requirements for nuclear power plants and focus on questions concerning reliability, requisite qualification and organizational structures.

  17. Review of the safety concept for fusion reactor concepts and transferability of the nuclear fission regulation to potential fusion power plants

    International Nuclear Information System (INIS)

    This paper summarizes the current state of the art in science and technology of the safety concept for future fusion power plants (FPPs) and examines the transferability of the current nuclear fission regulation to the concepts of future fusion power plants. At the moment there exist only conceptual designs of future fusion power plants. The most detailed concepts with regards to safety aspects were found in the European Power Plant Conceptual Study (PPCS). The plant concepts discussed in the PPCS are based on magnetic confinement of the plasma. The safety concept of fusion power plants, which has been developed during the last decades, is based on the safety concepts of installations with radioactive inventories, especially nuclear fission power plants. It applies the concept of defence in depth. However, there are specific differences between the implementations of the safety concepts due to the physical and technological characteristics of fusion and fission. It is analysed whether for fusion a safety concept is required comparable to the one of fission. For this the consequences of a purely hypothetical release of large amounts of the radioactive inventory of a fusion power plant and a fission power plant are compared. In such an event the evacuation criterion outside the plant is exceeded by several orders of magnitude for a fission power plant. For a fusion power plant the expected radiological consequences are of the order of the evacuation criterion. Therefore, a safety concept is also necessary for fusion to guarantee the confinement of the radioactive inventory. The comparison between the safety concepts for fusion and fission shows that the fundamental safety function ''confinement of the radioactive materials'' can be transferred directly in a methodical way. For a fusion power plant this fundamental safety function is based on both, physical barriers as well as on active retention functions. After the termination of the fusion

  18. Human metabolism and ecological transfer of radioactive caesium. Comparative studies of Chernobyl debris and nuclear weapons fallout, in southern Sweden and in Bryansk, Russia

    Energy Technology Data Exchange (ETDEWEB)

    Raeaef, C.L

    2000-05-01

    The whole-body content of radiocaesium was measured in a South Swedish urban group of people residing in the city of Lund between 1960 and 1994. The results from the survey have been analysed in order to estimate the ecological half time, T{sub eff,eco} of fallout radiocaesium, and the aggregate transfer from ground deposition to man in the region. After 1987, the biological half times, T{sub e} of {sup 137}Cs and {sup 40}K in man were also determined in the reference group through whole-body content measurements in combination with 24-hour urine sampling. Relationships between 24-hour urinary excretion and body burden of {sup 137}Cs in the group together with data from the literature were then applied to urine samples collected in 1994 and 1995 from adult subjects living in the highly contaminated region of Bryansk, Russia, in order to estimate their average body burden of {sup 137}Cs. The equivalent biological half-time for {sup 137}Cs in females of the Lund reference group was, on average 66{+-}3 d, which agrees with other findings, whereas the value for the males, 81{+-}4 d, was, on average, significantly lower than what is found in the literature. This is partly explained by the elevated mean age and relatively low mean body muscle mass of the males investigated. The {sup 137}Cs from nuclear weapons tests in the 1950s and 1960s still gave a significant contribution to the total {sup 137}Cs levels in man during the post-Chernobyl study period (1987-1994). About 10% of the peak post-Chernobyl concentration level of {sup 137}Cs (3.5-4 Bq/kg) in 1987, was attributed to pre-Chernobyl {sup 137}Cs. The effective ecological half-time for {sup 137}Cs from Chernobyl was found to be 1.8{+-}0.2 y. The time-integrated aggregate transfer of {sup 137}Cs from ground deposition to mean activity concentration in man was estimated to be 0.4 Bq/kg/kBq/m{sup 2}. These values may be compared with an effective ecological half-time of 1.3 years found in the Lund reference group in

  19. Review of the safety concept for fusion reactor concepts and transferability of the nuclear fission regulation to potential fusion power plants

    Energy Technology Data Exchange (ETDEWEB)

    Raeder, Juergen; Weller, Arthur; Wolf, Robert [Max-Planck-Institut fuer Plasmaphysik (IPP), Garching (Germany); Jin, Xue Zhou; Boccaccini, Lorenzo V.; Stieglitz, Robert; Carloni, Dario [Karlsruher Institute fuer Technologie (KIT), Eggenstein-Leopoldshafen (Germany); Pistner, Christoph [Oeko-Institut e.V., Darmstadt (Germany); Herb, Joachim [Gesellschaft fuer Anlagen- und Reaktorsicherheit, Koeln (Germany)

    2016-01-15

    This paper summarizes the current state of the art in science and technology of the safety concept for future fusion power plants (FPPs) and examines the transferability of the current nuclear fission regulation to the concepts of future fusion power plants. At the moment there exist only conceptual designs of future fusion power plants. The most detailed concepts with regards to safety aspects were found in the European Power Plant Conceptual Study (PPCS). The plant concepts discussed in the PPCS are based on magnetic confinement of the plasma. The safety concept of fusion power plants, which has been developed during the last decades, is based on the safety concepts of installations with radioactive inventories, especially nuclear fission power plants. It applies the concept of defence in depth. However, there are specific differences between the implementations of the safety concepts due to the physical and technological characteristics of fusion and fission. It is analysed whether for fusion a safety concept is required comparable to the one of fission. For this the consequences of a purely hypothetical release of large amounts of the radioactive inventory of a fusion power plant and a fission power plant are compared. In such an event the evacuation criterion outside the plant is exceeded by several orders of magnitude for a fission power plant. For a fusion power plant the expected radiological consequences are of the order of the evacuation criterion. Therefore, a safety concept is also necessary for fusion to guarantee the confinement of the radioactive inventory. The comparison between the safety concepts for fusion and fission shows that the fundamental safety function ''confinement of the radioactive materials'' can be transferred directly in a methodical way. For a fusion power plant this fundamental safety function is based on both, physical barriers as well as on active retention functions. After the termination of the fusion

  20. Review of the safety concept for fusion reactor concepts and transferability of the nuclear fission regulation to potential fusion power plants

    Energy Technology Data Exchange (ETDEWEB)

    Raeder, Juergen; Weller, Arthur; Wolf, Robert [Max-Planck-Institut fuer Plasmaphysik (IPP), Garching (Germany); Jin, Xue Zhou; Boccaccini, Lorenzo V.; Stieglitz, Robert; Carloni, Dario [Karlsruher Institute fuer Technologie (KIT), Eggenstein-Leopoldshafen (Germany); Pistner, Christoph [Oeko-Institut e.V., Darmstadt (Germany); Herb, Joachim [Gesellschaft fuer Anlagen- und Reaktorsicherheit, Koeln (Germany)

    2016-01-15

    This paper summarizes the current state of the art in science and technology of the safety concept for future fusion power plants (FPPs) and examines the transferability of the current nuclear fission regulation to the concepts of future fusion power plants. At the moment there exist only conceptual designs of future fusion power plants. The most detailed concepts with regards to safety aspects were found in the European Power Plant Conceptual Study (PPCS). The plant concepts discussed in the PPCS are based on magnetic confinement of the plasma. The safety concept of fusion power plants, which has been developed during the last decades, is based on the safety concepts of installations with radioactive inventories, especially nuclear fission power plants. It applies the concept of defence in depth. However, there are specific differences between the implementations of the safety concepts due to the physical and technological characteristics of fusion and fission. It is analysed whether for fusion a safety concept is required comparable to the one of fission. For this the consequences of a purely hypothetical release of large amounts of the radioactive inventory of a fusion power plant and a fission power plant are compared. In such an event the evacuation criterion outside the plant is exceeded by several orders of magnitude for a fission power plant. For a fusion power plant the expected radiological consequences are of the order of the evacuation criterion. Therefore, a safety concept is also necessary for fusion to guarantee the confinement of the radioactive inventory. The comparison between the safety concepts for fusion and fission shows that the fundamental safety function ''confinement of the radioactive materials'' can be transferred directly in a methodical way. For a fusion power plant this fundamental safety function is based on both, physical barriers as well as on active retention functions. After the termination of the fusion

  1. Transcriptomic and genomic evidence for Streptococcus agalactiae adaptation to the bovine environment

    Science.gov (United States)

    2013-01-01

    Background Streptococcus agalactiae is a major cause of bovine mastitis, which is the dominant health disorder affecting milk production within the dairy industry and is responsible for substantial financial losses to the industry worldwide. However, there is considerable evidence for host adaptation (ecotypes) within S. agalactiae, with both bovine and human sourced isolates showing a high degree of distinctiveness, suggesting differing ability to cause mastitis. Here, we (i) generate RNAseq data from three S. agalactiae isolates (two putative bovine adapted and one human) and (ii) compare publicly available whole genome shotgun sequence data from an additional 202 isolates, obtained from six host species, to elucidate possible genetic factors/adaptations likely important for S. agalactiae growth and survival in the bovine mammary gland. Results Tests for differential expression showed distinct expression profiles for the three isolates when grown in bovine milk. A key finding for the two putatively bovine adapted isolates was the up regulation of a lactose metabolism operon (Lac.2) that was strongly correlated with the bovine environment (all 36 bovine sourced isolates on GenBank possessed the operon, in contrast to only 8/151 human sourced isolates). Multi locus sequence typing of all genome sequences and phylogenetic analysis using conserved operon genes from 44 S. agalactiae isolates and 16 additional Streptococcus species provided strong evidence for acquisition of the operon via multiple lateral gene transfer events, with all Streptococcus species known to be major causes of mastitis, identified as possible donors. Furthermore, lactose fermentation tests were only positive for isolates possessing Lac.2. Combined, these findings suggest that lactose metabolism is likely an important adaptation to the bovine environment. Additional up regulation in the bovine adapted isolates included genes involved in copper homeostasis, metabolism of purine, pyrimidine

  2. Detection of a Novel Bovine Lymphotropic Herpesvirus

    OpenAIRE

    Rovnak, Joel; Quackenbush, Sandra L.; Reyes, Richard A.; Baines, Joel D.; Parrish, Colin R.; Casey, James W.

    1998-01-01

    Degenerate PCR primers which amplify a conserved region of the DNA polymerase genes of the herpesvirus family were used to provide sequence evidence for a new bovine herpesvirus in bovine B-lymphoma cells and peripheral blood mononuclear cells (PBMC). The sequence of the resultant amplicon was found to be distinct from those of known herpesvirus isolates. Alignment of amino acid sequences demonstrated 70% identity with ovine herpesvirus 2, 69% with alcelaphine herpesvirus 1, 65% with bovine h...

  3. Bovine Chymosin: A Computational Study of Recognition and Binding of Bovine κ-Casein

    DEFF Research Database (Denmark)

    Palmer, David S.; Christensen, Anders Uhrenholt; Sørensen, Jesper;

    2010-01-01

    Bovine chymosin is an aspartic protease that selectively cleaves the milk protein κ-casein. The enzyme is widely used to promote milk clotting in cheese manufacturing. We have developed models of residues 97-112 of bovine κ-casein complexed with bovine chymosin, using ligand docking, conformation...

  4. Measuring ligand-dependent and ligand-independent interactions between nuclear receptors and associated proteins using Bioluminescence Resonance Energy Transfer (BRET2)

    OpenAIRE

    Koterba, Kristen L.; Rowan, Brian G.

    2006-01-01

    Bioluminescent resonance energy transfer (BRET2) is a recently developed technology for the measurement of protein-protein interactions in a live, cell-based system. BRET2 is characterized by the efficient transfer of excited energy between a bioluminescent donor molecule (Renilla luciferase) and a fluorescent acceptor molecule (a mutant of Green Fluorescent Protein (GFP2)). The BRET2 assay offers advantages over fluorescence resonance energy transfer (FRET) because it does not require an ext...

  5. Mycobacterium bovis (Bovine Tuberculosis) in Humans

    Science.gov (United States)

    Mycobacterium bovis (Bovine Tuberculosis) in Humans What is Mycobacterium bovis ? In the United States, the majority of tuberculosis (TB) cases in people are caused by Mycobacterium tuberculosis ( ...

  6. Aspects of energetic substrate metabolism of in vitro and in vivo bovine embryos

    International Nuclear Information System (INIS)

    Although the metabolism of early bovine embryos has not been fully elucidated, several publications have addressed this important issue to improve culture conditions for cattle reproductive biotechnologies, with the ultimate goal of producing in vitro embryos similar in quality to those developing in vivo. Here, we review general aspects of bovine embryo metabolism in vitro and in vivo, and discuss the use of metabolic analysis of embryos produced in vitro to assess viability and predict a viable pregnancy after transference to the female tract

  7. Aspects of energetic substrate metabolism of in vitro and in vivo bovine embryos

    Directory of Open Access Journals (Sweden)

    D.K. de Souza

    2015-03-01

    Full Text Available Although the metabolism of early bovine embryos has not been fully elucidated, several publications have addressed this important issue to improve culture conditions for cattle reproductive biotechnologies, with the ultimate goal of producing in vitro embryos similar in quality to those developing in vivo. Here, we review general aspects of bovine embryo metabolism in vitro and in vivo, and discuss the use of metabolic analysis of embryos produced in vitro to assess viability and predict a viable pregnancy after transference to the female tract.

  8. Aspects of energetic substrate metabolism of in vitro and in vivo bovine embryos

    Energy Technology Data Exchange (ETDEWEB)

    Souza, D.K. de [Laboratório de Biotecnologia da Saúde, Faculdade de Medicina, Universidade de Brasília, Brasília, DF (Brazil); Faculdade da Ceilândia, Universidade de Brasília, Brasília, DF (Brazil); Salles, L.P. [Laboratório de Biotecnologia da Saúde, Faculdade de Medicina, Universidade de Brasília, Brasília, DF (Brazil); Departamento de Biologia Molecular, Instituto de Biologia, Universidade de Brasília, Brasília, DF (Brazil); Rosa e Silva, A.A.M. [Laboratório de Biotecnologia da Saúde, Faculdade de Medicina, Universidade de Brasília, Brasília, DF (Brazil)

    2015-01-23

    Although the metabolism of early bovine embryos has not been fully elucidated, several publications have addressed this important issue to improve culture conditions for cattle reproductive biotechnologies, with the ultimate goal of producing in vitro embryos similar in quality to those developing in vivo. Here, we review general aspects of bovine embryo metabolism in vitro and in vivo, and discuss the use of metabolic analysis of embryos produced in vitro to assess viability and predict a viable pregnancy after transference to the female tract.

  9. Communication Received from the Permanent Mission of Mexico to the International Atomic Energy Agency Regarding Guidelines for the Export of Nuclear Material, Equipment and Technology and the Guidelines for Transfers of Nuclear-related Dual-use Equipment, Materials, Software and Related Technology

    International Nuclear Information System (INIS)

    The Director General has received a note verbale dated 15 June 2012 from the Permanent Mission of Mexico to the International Atomic Energy Agency providing information on the decision of the Government of Mexico to act in accordance with the 'Guidelines for the Export of Nuclear Material, Equipment and Technology', issued as document INFCIRC/254/Rev.10/Part 1, including its Annexes, and with the 'Guidelines for Transfers of Nuclear-Related Dual-Use Equipment, Material, Software and Related Technology', issued as document INFCIRC/254/Rev.8/Part 2

  10. ¿Es posible la recuperación de especies silvestres extintas a través de la transferencia somática nuclear? Is the recovery of extinct wild species through somatic nuclear transfer possible?

    Directory of Open Access Journals (Sweden)

    Janet López-Saucedo

    2010-08-01

    technology approaches. However, considering that Mexico is one of the megadiverse countries with a great number of species of threatened mammals, biotechnological tools are urgently needed for conservation research of Mexican species. Furthermore, these tools need to be utilized and developed by our own scientific Latin-American community. In this note, we discuss the scientific advances that have proposed the use of somatic nuclear transfer as a conservation tool, its real possibilities, the needs and suggestions for its development in threatened Mexican / Latin-American mammal species that should allow modern biotechnology to turn into one more tool in the battle of biological conservation.

  11. Nuclear knowledge management

    International Nuclear Information System (INIS)

    The management of nuclear knowledge has emerged as a growing challenge in recent years. The need to preserve and transfer nuclear knowledge is compounded by recent trends such as ageing of the nuclear workforce, declining student numbers in nuclear-related fields, and the threat of losing accumulated nuclear knowledge. Addressing these challenges, the IAEA promotes a 'knowledge management culture' through: - Providing guidance for policy formulation and implementation of nuclear knowledge management; - Strengthening the contribution of nuclear knowledge in solving development problems, based on needs and priorities of Member States; - Pooling, analysing and sharing nuclear information to facilitate knowledge creation and its utilization; - Implementing effective knowledge management systems; - Preserving and maintaining nuclear knowledge; - Securing sustainable human resources for the nuclear sector; and - Enhancing nuclear education and training

  12. Technology transfer in CANDU marketing

    International Nuclear Information System (INIS)

    The author discusses how the CANDU system lends itself to technology transfer, the scope of CANDU technology transfer, and the benefits and problems associated with technology transfer. The establishment of joint ventures between supplier and client nations offers benefits to both parties. Canada can offer varying technology transfer packages, each tailored to a client nation's needs and capabilities. Such a package could include all the hardware and software necessary to develop a self-sufficient nuclear infrastructure in the client nation

  13. Bovine Necrotic Vulvovaginitis Associated with Porphyromonas levii

    OpenAIRE

    Elad, Daniel; Friedgut, Orly; Alpert, Nir; Stram, Yehuda; Lahav, Dan; Tiomkin, Doron; Avramson, Miriam; Grinberg, Kalia; Bernstein, Michael

    2004-01-01

    An outbreak of bovine necrotic vulvovaginitis associated with Porphyromonas levii, an emerging animal and human pathogen, affected 32 cows on a dairy farm in the northeast of Israel. Five animals had to be culled. This report appears to be the first that associates P. levii with bovine necrotic vulvovagnitis.

  14. In vitro production of bovine embryos

    DEFF Research Database (Denmark)

    Stroebech, L.; Mazzoni, Gianluca; Pedersen, Hanne Skovsgaard;

    2015-01-01

    In vitro production (IVP) of bovine embryos has become a widespread technology implemented in cattle breeding and production. The implementation of genomic selection and systems biology adds great dimensions to the impact of bovine IVP. The physical procedures included in the IVP process can still...

  15. Design and Construction of Chimeric VP8-S2 Antigen for Bovine Rotavirus and Bovine Coronavirus

    Science.gov (United States)

    Nasiri, Khadijeh; Nassiri, Mohammadreza; Tahmoorespur, Mojtaba; Haghparast, Alireza; Zibaee, Saeed

    2016-01-01

    Purpose: Bovine Rotavirus and Bovine Coronavirus are the most important causes of diarrhea in newborn calves and in some other species such as pigs and sheep. Rotavirus VP8 subunit is the major determinant of the viral infectivity and neutralization. Spike glycoprotein of coronavirus is responsible for induction of neutralizing antibody response. Methods: In the present study, several prediction programs were used to predict B and T-cells epitopes, secondary and tertiary structures, antigenicity ability and enzymatic degradation sites. Finally, a chimeric antigen was designed using computational techniques. The chimeric VP8-S2 antigen was constructed. It was cloned and sub-cloned into pGH and pET32a(+) expression vector. The recombinant pET32a(+)-VP8-S2 vector was transferred into E.oli BL21CodonPlus (DE3) as expression host. The recombinant VP8-S2 protein was purified by Ni-NTA chromatography column. Results: The results of colony PCR, enzyme digestion and sequencing showed that the VP8-S2 chimeric antigen has been successfully cloned and sub-cloned into pGH and pET32a(+).The results showed that E.coli was able to express VP8-S2 protein appropriately. This protein was expressed by induction of IPTG at concentration of 1mM and it was confirmed by Ni–NTA column, dot-blotting analysis and SDS-PAGE electrophoresis. Conclusion: The results of this study showed that E.coli can be used as an appropriate host to produce the recombinant VP8-S2 protein. This recombinant protein may be suitable to investigate to produce immunoglobulin, recombinant vaccine and diagnostic kit in future studies after it passes biological activity tests in vivo in animal model and or other suitable procedure. PMID:27123423

  16. Scientific Opinion on bovine lactoferrin

    OpenAIRE

    EFSA Panel on Dietetic Products, Nutrition and Allergies (NDA)

    2012-01-01

    Following a request from the European Commission, the EFSA Panel on Dietetic Products, Nutrition and Allergies (NDA) was asked to carry out the additional assessment for ‘lactoferrin’ as a food ingredient in the context of Regulation (EC) No 258/97 taking into account the comments and objections of a scientific nature raised by Member States. Bovine lactoferrin (bLF) is a protein that occurs naturally in cow’s milk. The applicant intends to market bLF that is isolated from cheese whe...

  17. Models of bovine babesiosis including juvenile cattle.

    Science.gov (United States)

    Saad-Roy, C M; Shuai, Zhisheng; van den Driessche, P

    2015-03-01

    Bovine Babesiosis in cattle is caused by the transmission of protozoa of Babesia spp. by ticks as vectors. Juvenile cattle (resistance to Bovine Babesiosis, rarely show symptoms, and acquire immunity upon recovery. Susceptibility to the disease varies between breeds of cattle. Models of the dynamics of Bovine Babesiosis transmitted by the cattle tick that include these factors are formulated as systems of ordinary differential equations. Basic reproduction numbers are calculated, and it is proved that if these numbers are below the threshold value of one, then Bovine Babesiosis dies out. However, above the threshold number of one, the disease may approach an endemic state. In this case, control measures are suggested by determining target reproduction numbers. The percentage of a particular population (for example, the adult bovine population) needed to be controlled to eradicate the disease is evaluated numerically using Columbia data from the literature. PMID:25715822

  18. Clinical applications of bovine colostrum therapy

    DEFF Research Database (Denmark)

    Rathe, Mathias; Müller, Klaus; Sangild, Per Torp;

    2014-01-01

    Bovine colostrum, the first milk that cows produce after parturition, contains high levels of growth factors and immunomodulatory components. Some healthy and diseased individuals may gain health benefits by consuming bovine colostrum as a food supplement. This review provides a systematic...... to populations, outcomes, and methodological quality, as judged by the Jadad assessment tool. Many studies used surrogate markers to study the effects of bovine colostrum. Studies suggesting clinical benefits of colostrum supplementation were generally of poor methodological quality, and results could...... not be confirmed by other investigators. Bovine colostrum may provide gastrointestinal and immunological benefits, but further studies are required before recommendations can be made for clinical application. Animal models may help researchers to better understand the mechanisms of bovine colostrum supplementation...

  19. Bovine in vitro embryo production : An overview

    Directory of Open Access Journals (Sweden)

    V. S. Suthar

    Full Text Available Dairy industry perfected the application of the first reproductive biotechnology, i.e. artificial insemination (AI - a great success story and also remains the user of embryo transfer technology (ETT. In addition, recently the researchers taking interest to embraced the field of Transvaginal OocyteRecovery (TVOR and in vitro production (IVEP of embryos. IVF provides the starting point for the generation of reproductive material for a number of advanced reproduction techniques including sperm microinjection and nuclear transfer (cloning. In several countries commercial IVF facilities are already being employed by cattle ET operators. Various research groups have reported on modification of TVOR technique to give greater efficiency. Much research is still needed in domestic animal (Especially Indian species on mechanisms controlling embryo development and on development of totally in vitro system for embryo culture. [Vet World 2009; 2(12.000: 478-479`

  20. Proceedings of the ANS/ASME/NRC international topical meeting on nuclear reactor thermal-hydraulics: fundamental aspects of two-phase flow and boiling heat transfer

    International Nuclear Information System (INIS)

    Separate abstracts are included for each of the papers presented concerning critical flow of two-phase mixtures; two-phase flow instrumentation; critical heat flux and effects of local disturbances; heat transfer and rewetting during reflood; hydrodynamic mechanisms in boiling heat transfer; and entrainment and droplet deposition in two-phase flow. Five papers have been previously abstracted and input to the data base

  1. Insights on bovine genetic engineering and cloning

    Directory of Open Access Journals (Sweden)

    Fabiana F. Bressan

    2013-12-01

    Full Text Available Transgenic technology has become an essential tool for the development of animal biotechnologies, and animal cloning through somatic cell nuclear transfer (SCNT enabled the generation of genetically modified animals utilizing previously modified and selected cell lineages as nuclei donors, assuring therefore the generation of homogeneous herds expressing the desired modification. The present study aimed to discuss the use of SCNT as an important methodology for the production of transgenic herds, and also some recent insights on genetic modification of nuclei donors and possible effects of gene induction of pluripotency on SCNT.

  2. Updating of the bovine neosporosis

    Directory of Open Access Journals (Sweden)

    Alexander Martínez Contreras

    2012-06-01

    Full Text Available In the fields of Medicine and bovine production, there is a wide variety of diseases affecting reproduction, in relation to the number of live births, the interval between births and open days, among others. Some of these diseases produce abortions and embryonic death, which explain the alteration of reproductive parameters. Many of these diseases have an infectious origin, such as parasites, bacteria, viruses and fungi, which are transmitted among animals. Besides, some of them have zoonotic features that generate problems to human health. Among these agents, the Neospora caninum, protozoan stands out. Its life cycle is fulfilled in several species of animals like the dog and the coyote. These two act as its definitive hosts and the cattle as its intermediary host. The Neospora caninum causes in the infected animals, reproductive disorders, clinical manifestations and decreased production which affects productivity of small, medium and large producers. Because of this, diagnostic techniques that allow understanding the epidemiological behavior of this disease have been developed. However in spite of being a major agent in the bovine reproductive health, few studies have been undertaken to determine the prevalence of this agent around the world. Therefore, the objective of this review was to collect updated information on the behavior of this parasite, targeting its epidemiology, its symptoms, its impact on production and the methods of its control and prevention.

  3. Adipogenesis of bovine perimuscular preadipocytes

    International Nuclear Information System (INIS)

    In this study, non-transformed progeny adipofibroblasts, derived from mature adipocyte dedifferentiation, was used as a novel in vitro model to study adipogenic gene expression in cattle. Adipofibroblasts from dedifferentiated mature perimuscular fat (PMF) tissue were cultured with differentiation stimulants until the cells exhibited morphological differentiation. Treated cells were harvested from day 2 to 16 for RNA extraction, whereas control cells were cultured without addition of stimulants. Results from time course gene expression assays by quantitative real-time PCR revealed that peroxisome proliferator-activated receptor gamma (PPAR-γ), sterol regulatory element binding protein 1 (SREBP-1) and their six down-stream genes were co-expressed at day 2 post-differentiation induction. When compared to other adipogenesis culture systems, the adipogenic gene expression of bovine PMF adipofibroblasts culture was different, especially to the rodent model. Collectively, these results demonstrated PPAR-γ and SREBP-1 cooperatively play a key role to regulate the re-differentiation of bovine adipofibroblasts, during early conversion stages in vitro

  4. Capping of Silybin with β-Cyclodextrin Influences its Binding with Bovine Serum Albumin: A Study by Fluorescence Spectroscopy and Molecular Modeling

    International Nuclear Information System (INIS)

    The association of silybin with β-cyclodextrin and its influence on silybin's binding with bovine serum albumin are reported. The stoichiometry, binding constant, and the structure of silybin-β-cyclodextrin inclusion complex are reported. The titrations of silybin with bovine serum albumin in the absence and presence of β-cyclodextrin are carried out and the differences in binding strengths are discussed. Molecular modeling is used to optimize the sites and mode of binding of silybin with bovine serum albumin. Forster resonance energy transfer is calculated and the proximity of interacting molecules is reported in the presence and absence of β-cyclodextrin

  5. Capping of Silybin with β-Cyclodextrin Influences its Binding with Bovine Serum Albumin: A Study by Fluorescence Spectroscopy and Molecular Modeling

    Energy Technology Data Exchange (ETDEWEB)

    Natesan, Sudha; Sowrirajan, Chandrasekaran; Dhanaraj, Premnath; Enoch, Israel V. M. V. [Karunya Univ., Tamil Nadu (India)

    2014-07-15

    The association of silybin with β-cyclodextrin and its influence on silybin's binding with bovine serum albumin are reported. The stoichiometry, binding constant, and the structure of silybin-β-cyclodextrin inclusion complex are reported. The titrations of silybin with bovine serum albumin in the absence and presence of β-cyclodextrin are carried out and the differences in binding strengths are discussed. Molecular modeling is used to optimize the sites and mode of binding of silybin with bovine serum albumin. Forster resonance energy transfer is calculated and the proximity of interacting molecules is reported in the presence and absence of β-cyclodextrin.

  6. Radiative heat transfer

    CERN Document Server

    Modest, Michael F

    2013-01-01

    The third edition of Radiative Heat Transfer describes the basic physics of radiation heat transfer. The book provides models, methodologies, and calculations essential in solving research problems in a variety of industries, including solar and nuclear energy, nanotechnology, biomedical, and environmental. Every chapter of Radiative Heat Transfer offers uncluttered nomenclature, numerous worked examples, and a large number of problems-many based on real world situations-making it ideal for classroom use as well as for self-study. The book's 24 chapters cover the four major areas in the field: surface properties; surface transport; properties of participating media; and transfer through participating media. Within each chapter, all analytical methods are developed in substantial detail, and a number of examples show how the developed relations may be applied to practical problems. It is an extensive solution manual for adopting instructors. Features: most complete text in the field of radiative heat transfer;...

  7. Cellular and molecular deviations in bovine in vitro-produced embryos are related to the large offspring syndrome

    NARCIS (Netherlands)

    Lazzari, G.; Wrenzycki, C.; Herrmann, D.; Duchi, R.; Kruip, T.; Niemann, H.; Galli, C.

    2002-01-01

    The large offspring syndrome (LOS) is observed in bovine and ovine offspring following transfer of in vitro-produced (IVP) or cloned embryos and is characterized by a multitude of pathologic changes, of which extended gestation length and increased birthweight are predominant features. In the presen

  8. Immunoprophylaxis of bovine respiratory syndrome

    Directory of Open Access Journals (Sweden)

    Rogan Dragan

    2010-01-01

    Full Text Available Bovine Respiratory Syndrome (BRS is a multifactorial disease caused by the interaction of infective agents, the environment and the individual immunological response of animals in the herd. Despite five decades of research on BRS, no clear understanding of how environmental factors influence pathogenic outcomes of the disease has been defined. As such, the development of immunoprophylaxis and vaccine programmes to prevent outbreaks of BRS in cattle has not been successful. The current paper discusses vaccination programmes for all categories of cattle and presents a review of existing vaccines being used for immunoprophylaxis of respiratory syndrome in cattle and discusses the advantages and disadvantages of the currently used vaccines and vaccination programmes. Lastly, a discussion detailing the design of future perfect vaccines is presented.

  9. Search for the genome of bovine herpesvirus types 1, 4 and 5 in bovine semen

    OpenAIRE

    P.E. Morán; Favier, P.A.; Lomónaco, M.; Catena, M.C.; M.L. Chiapparrone; Odeón, A.C.; Verna, A.E.; S.E. Pérez

    2013-01-01

    Bovine herpesvirus type 1 (BoHV-1) causes respiratory and reproductive disorders in cattle. Recently, bovine herpesvirus type 5 (BoHV-5) and bovine herpesvirus type 4 (BoHV-4) have been identified to be associated with genital disease. In this study, the presence of the genome of BoHV-1, BoHV-4 and BoHV-5 in bovine semen of Argentinean and international origin was analyzed by PCR assays. The most important finding of this study is the detection of the genome of BoHV-1 and BoHV-4 in semen of b...

  10. Application of a nuclear localization signal gene in transgene mice

    Institute of Scientific and Technical Information of China (English)

    2002-01-01

    Efficient gene transfer by cytoplasm co-injec- tion will offer a powerful means for transgenic animals. Using co-injection in cytoplasm, two independent gene constructs, including bovine (?-s1-casein-hG-CSF and a mammal expression vector expressing a nuclear localization signal (mNLS), were introduced into fertilized mouse eggs. The target gene construct was docked into host nucleus probably by the nuclear localization signal. Transgene mice have been obtained at 58% (29/50) of integration ratio. Expression level of the positive transgene mice was detected by Western blotting. Maximal expression of human G-CSF was estimated about 540 mg/L of milk. The expression ratio was up to 75% (9/12). The results here have important practical implications for the generation of mammary gland bioreactors and other transgene studies. Co-injection of a target gene with an expression vector of a mammal nuclear localization signal by cytoplasm appears to be a useful, efficient and easy strategy for generating transgenic animals, which may be able to substitute the routine method of pronucleus-injection of fertilized eggs.

  11. 核电项目建造阶段中的焊接工艺评定转移%Welding procedure qualification transference to different project of nuclear power item during the construction period

    Institute of Scientific and Technical Information of China (English)

    马新朝

    2011-01-01

    论述了核电站在建造阶段焊接工艺评定报告由已完成建造任务的核电项目至正在建设中的核电项目中的焊接工艺评定报告的转移情况,分析了工艺评定转移的可行性,转移后施工单位、监理单位、政府监督单位所应关注的重点,突出了质量保证及质量控制,更关注了政府监督单位(核安全局及地区核与辐射监督站)的监督职能.焊接工艺评定的有效转移,一方面节约了重复评定时的人力和物力投入,降低了建造成本;另一方面有力的推动了工程的顺利建设,促进焊接技术及经验的持续发展,从而提离了管理水平.%The paper shows us the Welding producer report transference to different project of nuclear power item during the construction period,analyses the practicability to the producer report transference. After transference,the paper discusses the focal point to welding in construction enterprises,surveillance management corporation as well as the responsible for supervision departments of the Government,give prominence to the Quality assurance and Quality control. The Nuclear Safety Bureau and the Area Departments Responsible for Supervision on radiation should give more attention to the surveillance on transference.The effective transfer of PQR can reduce expenses ,material and manpower in duplicate welding procedure qualification,reduce construction costs ;the other side,which can strongly promotes the project construction,accelerate the welding technology and experience continue development,and improve the management standards.

  12. International Youth Nuclear Congress 2000: Youth, Future, Nuclear. Transactions

    International Nuclear Information System (INIS)

    The publication has been set up as abstracts of the meeting dealing with different nuclear problems. In the work of the International Youth Nuclear Congress 2000 participated more than 200 young scientific works from 28 countries. The address discusses the following issues: Nuclear education and transfer of know-how; Nuclear technology; Political aspects; Environment and safety; Communication and public perception; Economics; Nuclear programs and technical cooperation; Fuel Cycle Challenges

  13. Density content of nuclear symmetry energy from nuclear observables

    Indian Academy of Sciences (India)

    B K Agrawal

    2014-11-01

    The nuclear symmetry energy at a given density measures the energy transferred in converting symmetric nuclear matter into the pure neutron matter. The density content of nuclear symmetry energy remains poorly constrained. Our recent results for the density content of the nuclear symmetry energy, around the saturation density, extracted using experimental data for accurately known nuclear masses, giant resonances and neutron-skin thickness in heavy nuclei are summarized.

  14. Analysis of Chromatin Attachment and Partitioning Functions of Bovine Papillomavirus Type 1 E2 Protein

    OpenAIRE

    Abroi, Aare; Ilves, Ivar; Kivi, Sirje; Ustav, Mart

    2004-01-01

    Recent studies have suggested that the tethering of viral genomes to host cell chromosomes could provide one of the ways to achieve their nuclear retention and partitioning during extrachromosomal maintenance in dividing cells. The data we present here provide firm evidence that the partitioning of the bovine papillomavirus type 1 (BPV1) genome is dependent on the chromatin attachment process mediated by viral E2 protein and its multiple binding sites. On the other hand, the attachment of E2 ...

  15. Interfacial behaviour of bovine testis hyaluronidase

    OpenAIRE

    Belem-Gonçalves, Silvia; Tsan, Pascale; Lancelin, Jean-Marc; Alves, Tito L. M.; Salim, Vera M.; Besson, Françoise

    2006-01-01

    Abstract The interfacial properties of bovine testicular hyaluronidase were suggested by demonstrating the association of hyaluronidase activity with membranes prepared from bovine testis. Protein adsorption to the air/water interface was investigated using surface pressure-area isotherms. Whatever the way to obtain interfacial films (protein injection or deposition), the hyaluronidase exhibited a significant affinity for the air/water interface. The isotherm obtained 180 min after...

  16. Bovine viral diarrhea virus: biotypes and disease.

    OpenAIRE

    Deregt, D; Loewen, K G

    1995-01-01

    Bovine viral diarrhea virus continues to produce significant economic losses for the cattle industry and challenges investigators with the complexity of diseases it produces and the mechanisms by which it causes disease. This paper updates and attempts to clarify information regarding the roles of noncytopathic and cytopathic bovine viral diarrhea viruses in persistent infections and mucosal disease. It also covers, in brief, what is known of the new diseases: thrombocytopenia and hemorrhagic...

  17. Trophoblast Major Histocompatibility Complex Class I Expression Is Associated with Immune-Mediated Rejection of Bovine Fetuses Produced by Cloning.

    Science.gov (United States)

    Rutigliano, Heloisa M; Thomas, Aaron J; Wilhelm, Amanda; Sessions, Benjamin R; Hicks, Brady A; Schlafer, Donald H; White, Kenneth L; Davies, Christopher J

    2016-08-01

    Trophoblast cells from bovine somatic cell nuclear transfer (SCNT) conceptuses express major histocompatibility complex class I (MHC-I) proteins early in gestation, and this may be one cause of the significant first-trimester embryonic mortality observed in these pregnancies. MHC-I homozygous-compatible (n = 9), homozygous-incompatible (n = 8), and heterozygous-incompatible (n = 5) SCNT pregnancies were established. The control group consisted of eight pregnancies produced by artificial insemination. Uterine and placental samples were collected on Day 35 ± 1 of pregnancy, and expression of MHC-I, leukocyte markers, and cytokines were examined by immunohistochemistry. Trophoblast cells from all SCNT pregnancies expressed MHC-I, while trophoblast cells from age-matched control pregnancies were negative for MHC-I expression. Expression of MHC-I antigens by trophoblast cells from SCNT pregnancies was associated with lymphocytic infiltration in the endometrium. Furthermore, MHC-I-incompatible conceptuses, particularly the heterozygous-incompatible ones, induced a more pronounced lymphocytic infiltration than MHC-I-compatible conceptuses. Cells expressing cluster of differentiation (CD) 3, gamma/deltaTCR, and MHC-II were increased in the endometrium of SCNT pregnancies compared to the control group. CD4(+) lymphocytes were increased in MHC-I-incompatible pregnancies compared to MHC-I-compatible and control pregnancies. CD8(+), FOXP3(+), and natural killer cells were increased in MHC-I heterozygous-incompatible SCNT pregnancies compared to homozygous SCNT and control pregnancies. PMID:27385783

  18. Subcellular Localization and Polymorphism of Bovine FABP4 in Bovine Intramuscular Adipocytes.

    Science.gov (United States)

    Yonekura, Shinichi; Hirota, Shohei; Miyazaki, Honami; Tokutake, Yukako

    2016-01-01

    Fatty acid binding protein 4 (FABP4) I74 V, a gene polymorphism associated with unsaturated fatty acid contents, was discovered in Japanese Black cattle. Individuals with FABP4 I/I genotype contain a significantly high level of palmitoleic acid compared to those with FABP4 V/V genotype. It remains unknown how the FABP4 polymorphism leads to different palmitoleic acid contents. We overexpressed FABP4 of different genotypes in bovine intramuscular preadipocytes and examined whether the intracellular localization of FABP4 and the expression levels of lipid metabolism-related genes were different among cells expressing different genotypes. Nuclear localization was observed for the FABP4 V/V, while the FABP4 I/I almost did not. The cells expressing FABP4 of different genotypes were comparable in terms of the expression levels of genes involved in lipid metabolism. FABP4 I/I was localized in most of the lipid droplets 4 days after differentiation induction, whereas approximately 25% lipid droplet co-localized with FABP4 in cells expressing FABP4 V/V. The lipid droplet size increased when palmitoleic acid was added compared to the size observed when palmitic acid was added. These results suggest that lipid droplet enlargement caused by palmitoleic acid and genotype-dependent differences in the fatty acid transporting capacity underlie variations in palmitoleic acid content among FABP4 polymorphisms. PMID:26913550

  19. Bovine endometrial stromal cells display osteogenic properties

    Directory of Open Access Journals (Sweden)

    Cavirani Sandro

    2008-12-01

    Full Text Available Abstract The endometrium is central to mammalian fertility. The endometrial stromal cells are very dynamic, growing and differentiating throughout the estrous cycle and pregnancy. In humans, stromal cells appear to have progenitor or stem cell capabilities and the cells can even differentiate into bone. It is not clear whether bovine endometrial stromal cells exhibit a similar phenotypic plasticity. So, the present study tested the hypothesis that bovine endometrial stromal cells could be differentiated along an osteogenic lineage. Pure populations of bovine stromal cells were isolated from the endometrium. The endometrial stromal cell phenotype was confirmed by morphology, prostaglandin secretion, and susceptibility to viral infection. However, cultivation of the cells in standard endometrial cell culture medium lead to a mesenchymal phenotype similar to that of bovine bone marrow cells. Furthermore, the endometrial stromal cells developed signs of osteogenesis, such as alizarin positive nodules. When the stromal cells were cultured in a specific osteogenic medium the cells rapidly developed the characteristics of mineralized bone. In conclusion, the present study has identified that stromal cells from the bovine endometrium show a capability for phenotype plasticity similar to mesenchymal progenitor cells. These observations pave the way for further investigation of the mechanisms of stroma cell differentiation in the bovine reproductive tract.

  20. Activation of bovine neutrophils by Brucella spp.

    Science.gov (United States)

    Keleher, Lauren L; Skyberg, Jerod A

    2016-09-01

    Brucellosis is a globally important zoonotic infectious disease caused by gram negative bacteria of the genus Brucella. While many species of Brucella exist, Brucella melitensis, Brucella abortus, and Brucella suis are the most common pathogens of humans and livestock. The virulence of Brucella is largely influenced by its ability to evade host factors, including phagocytic killing mechanisms, which are critical for the host response to infection. The aim of this study was to characterize the bovine neutrophil response to virulent Brucella spp. Here, we found that virulent strains of smooth B. abortus, B. melitensis, B. suis, and virulent, rough, strains of Brucella canis possess similar abilities to resist killing by resting, or IFN-γ-activated, bovine neutrophils. Bovine neutrophils responded to infection with a time-dependent oxidative burst that varied little between Brucella spp. Inhibition of TAK1, or SYK kinase blunted the oxidative burst of neutrophils in response to Brucella infection. Interestingly, Brucella spp. did not induce robust death of bovine neutrophils. These results indicate that bovine neutrophils respond similarly to virulent Brucella spp. In addition, virulent Brucella spp., including naturally rough strains of B. canis, have a conserved ability to resist killing by bovine neutrophils. PMID:27436438

  1. The criterion for blanking-off heat-transfer tubes in the steam generators at VVER-based nuclear power plants based on the results of eddy-current examination

    Science.gov (United States)

    Lunin, V. P.; Zhdanov, A. G.; Chegodaev, V. V.; Stolyarov, A. A.

    2015-05-01

    The problem of defining the criterion for blanking off heat-transfer tubes in the steam generators at nuclear power plants on the basis of signals obtained from the standard multifrequency eddy-current examination is considered. The decision about blanking off one or another tube is presently made with reference to one parameter of the relevant signal at the working frequency, namely, with reference to its phase, which directly depends on the depth of the flaw being detected, i.e., a crack in the tube. The crack depth equal to 60% of the tube wall thickness is regarded to be the critical one, at which a decision about withdrawing such a tube out from operation (blanking off) must be taken. However, since mechanical tensile rupture tests of heat-transfer tubes show the possibility of their further use with such flaws, the secondary parameter of the signal, namely, its amplitude, must be used for determining the blanking-off criterion. The signals produced by the standard flow-type transducers in response to flaws in the form of a longitudinal crack having the depth and length within the limits permitted by the relevant regulations were calculated using 3D finite-element modeling. Based on the obtained results, the values of the eddy-current signal amplitude were determined, which, together with the signal phase value, form a new amplitude-phase criterion for blanking off heat-transfer tubes. For confirming the effectiveness of this technique, the algorithm for revealing the signal indications satisfying the proposed amplitude-phase criterion was tested on real signals obtained from operational eddy-current examination of the state of steam generator heat-transfer tubes carried out within the framework of planned preventive repair.

  2. A new measurement of the energy dependence of nuclear transparency for large momentum transfer 12C(p,2p) scattering

    Science.gov (United States)

    Leksanov, A.; Alster, J.; Asryan, G.; Averichev, Y.; Barton, D.; Baturin, V.; Bukhtojarova, N.; Carroll, A.; Schetkovsky, A.; Heppelmann, S.; Kawabata, T.; Malki, A.; Makdisi, Y.; Minina, E.; Navon, I.; Nicholson, H.; Ogawa, A.; Panebratsev, Y.; Piasetzky, E.; Shimanskiy, S.; Tang, A.; Watson, J. W.; Yoshida, H.; Zhalov, D.

    2000-12-01

    We present a new measurement of the energy dependence of nuclear transparency from AGS experiment E850, performed using the EVA solenoidal spectrometer, upgraded since 1995. Using a secondary beam from the AGS accelerator, we simultaneously measured pp elastic scattering from hydrogen and (p,2p) quasi-elastic scattering in carbon at incoming momenta of 5.9, 8.0, 9.0, 11.7 and 14.4 GeV/c. This incident momentum range corresponds to a Q2 region between 4.8 and 12.7 (GeV/c)2. The detector allowed us to do a complete kinematic analysis for the center-of-mass polar angles in the range 85°-90°. We report on the measured variation of the nuclear transparency with energy and compare the new results with previous measurements.

  3. FILLC - a subroutine to transfer nuclear group cross-sections from SIGMN blocks to data-arrays of neutron transport codes

    International Nuclear Information System (INIS)

    FILLC is a utility subroutine for use in the KAPROS versions of multigrop SN codes. Within KAPROS a couple of modules create and modify nuclear cross sections and store them in a so-called SIGMN file. FILLC stores group cross sections in an array C used by some SN codes with data provided in a SIGMN file. This report documents this interface with release number 3.4. (orig.)

  4. Deciphering the binding patterns and conformation changes upon the bovine serum albumin-rosmarinic acid complex.

    Science.gov (United States)

    Peng, Xin; Wang, Xiangchao; Qi, Wei; Huang, Renliang; Su, Rongxin; He, Zhimin

    2015-08-01

    Rosmarinic acid (RA) is an importantly and naturally occurring polyphenol from plants of the mint family with potent biological activities. Here, the in vitro interaction of RA with bovine serum albumin (BSA) has been investigated using various biophysical approaches as well as molecular modeling methods, to ascertain its binding mechanism and conformational changes. The fluorescence results demonstrated that the fluorescence quenching of BSA by RA was mainly the result of the formation of a ground state BSA-RA complex, and BSA had one high affinity RA binding site with a binding constant of 4.18 × 10(4) mol L(-1) at 298 K. Analysis of thermodynamic parameters revealed that hydrophobic and hydrogen bond interactions were the dominant intermolecular force in the complex formation. The primary binding site of RA in BSA (site I) had been identified by site marker competitive experiments. The distance between RA and the tryptophan residue of BSA was evaluated at 3.12 nm based on Förster's theory of non-radiation energy transfer. The UV-vis absorption, synchronous fluorescence, three-dimensional fluorescence, 8-anilino-1-naphthalenesulfonic acid (ANS) fluorescence, circular dichroism (CD), and Fourier transform infrared (FT-IR) spectra confirmed that the conformation and structure of BSA were altered in the presence of RA. Moreover, the nuclear magnetic spectroscopy showed that the aromatic groups of RA took part in the binding reaction during the BSA-RA complexation. In addition, the molecular picture of the interaction mechanism between BSA and RA at the atomic level was well examined by molecular docking and dynamics studies. In brief, RA can bind to BSA with noncovalent bonds in a relatively stable way, and these findings will be beneficial to the functional food research of RA. PMID:26146359

  5. DNA Methylation and Histone Acetylation Patterns in Cultured Bovine Adipose Tissue-Derived Stem Cells (BADSCs

    Directory of Open Access Journals (Sweden)

    Beheshteh Abouhamzeh

    2015-01-01

    Full Text Available Objective: Many studies have focused on the epigenetic characteristics of donor cells to improve somatic cell nuclear transfer (SCNT. We hypothesized that the epigenetic status and chromatin structure of undifferentiated bovine adipose tissue-derived stem cells (BADSCs would not remain constant during different passages. The objective of this study was to determine the mRNA expression patterns of DNA methyltransferases (DNMT1, DNMT3a, DNMT3b and histone deacetyltransferses (HDAC1, HDAC2, HDAC3 in BADSCs. In addition, we compared the measured levels of octamer binding protein-4 expression (OCT4 and acetylation of H3K9 (H3K9ac in BADSCs cultures and different passages in vitro. Materials and Methods: In this experimental study, subcutaneous fat was obtained from adult cows immediately post-mortem. Relative level of DNMTs and HDACs was examined using quantitative real time polymerase chain reaction (q-PCR, and the level of OCT4 and H3K9ac was analyzed by flow cytometry at passages 3 (P3, 5 (P5 and 7 (P7. Results: The OCT4 protein level was similar at P3 and P5 but a significant decrease in its level was seen at P7. The highest and lowest levels of H3K9ac were observed at P5 and P7, respectively. At P5, the expression of HDACs and DNMTs was significantly decreased. In contrast, a remarkable increase in the expression of DNMTs was observed at P7. Conclusion: Our data demonstrated that the epigenetic status of BADSCs was variable during culture. The P5 cells showed the highest level of stemness and multipotency and the lowest level of chromatin compaction. Therefore, we suggest that P5 cells may be more efficient for SCNT compared with other passages.

  6. Frequently Asked Questions on BSE (Bovine Spongiform Encephalopathy or Mad Cow Disease)

    Science.gov (United States)

    ... BSE / FAQ on BSE (Bovine Spongiform Encephalopathy or Mad Cow Disease) Programs Beginning Farmer and Rancher Development Farm Storage ... Asked Questions on BSE (Bovine Spongiform Encephalopathy or Mad Cow Disease) Q. What is Bovine Spongiform Encephalopathy? A. Bovine ...

  7. The Contribution of Infections with Bovine Viral Diarrhea Viruses to Bovine Respiratory Disease

    Science.gov (United States)

    The contribution of bovine viral diarrhea viruses (BVDV) to the development of bovine respiratory disease is the sum of a number of different factors. These factors include the contribution of acute uncomplicated BVDV infections, the high incidence of respiratory disease in animals persistently inf...

  8. Design and Construction of Chimeric VP8-S2 Antigen for Bovine Rotavirus and Bovine Coronavirus

    OpenAIRE

    Nasiri, Khadijeh; Nassiri, Mohammadreza; Tahmoorespur, Mojtaba; Haghparast, Alireza; Zibaee, Saeed

    2016-01-01

    Purpose: Bovine Rotavirus and Bovine Coronavirus are the most important causes of diarrhea in newborn calves and in some other species such as pigs and sheep. Rotavirus VP8 subunit is the major determinant of the viral infectivity and neutralization. Spike glycoprotein of coronavirus is responsible for induction of neutralizing antibody response.

  9. Bovine viral diarrhea virus: involvement in bovine respiratory disease and diagnostic challenges

    Science.gov (United States)

    This paper reviews the contribution of bovine viral diarrhea viruses (BVDV) to the development of Bovine Respiratory Disease (BRD). Veterinarians and producers generally consider BRD as one of the most significant diseases affecting production in the cattle industry. BRD can affect the performance (...

  10. Complete Genome Sequence of a Bovine Viral Diarrhea Virus 2 from Commercial Fetal Bovine Serum

    OpenAIRE

    Liu, Hua; Li, Yan; Gao, Mingchun; Wen, Kai; Jia, Ying; Liu, Xiaomei; Zhang, Wenlong; Ma, Bo; Wang, Junwei

    2012-01-01

    We isolated a bovine viral diarrhea virus (BVDV) from commercial fetal bovine serum and designated it HLJ-10. The complete genome is 12,284 nucleotides (nt); the open reading frame is 11,694 nt, coding 3,898 amino acids. Phylogenetic analysis indicated that this strain belongs to BVDV group 2.

  11. Genetic and physical mapping of the bovine X chromosome.

    Science.gov (United States)

    Yeh, C C; Taylor, J F; Gallagher, D S; Sanders, J O; Turner, J W; Davis, S K

    1996-03-01

    Three hundred eighty reciprocal backcross and F(2) full sib progeny from 33 families produced by embryo transfer from 77 Angus (Bos taurus), Brahman (Bos indicus), and F1 parents and grandparents were used to construct genetic maps of the bovine X and Y chromosomes. Ml individuals were scored for 15 microsatellite loci, with an average of 608 informative meioses per locus. The length of the bovine X chromosome genetic map was 118.7 cM (female only) and of the pseudoautosomal region was 13.0 cM (male only). The 15-marker framework map in Kosambi centimorgans is [BM6017-6.1 -TGLA89-35.8-TEXAN13-3.4-TGLA128-1.3 -BM2713 -21.1 -BM4604-2.4-BR215 - 12.9-TGLA68-10.0-BM4321 - 1.0-HEL14-4.9-TGLA15-2.3-INRA12O- 12.5-TGLA325- 1.6-MAF45-3.2-INRA3O], with an average interval of 7.91 cM. Clones containing pseudoautosomal or sex-linked microsatellites were isolated from a bovine bacterial artificial chromosome library and were physically mapped to bovine metaphase chromosomes by fluorescence in situ hybridization to orient the X and Y chromosome maps. BAC57, containing the pseudoautosomal microsatellite INRA3O, mapped to the distal end of the long arm of the X chromosome at q42-ter and to the short arm of the Y chromosome at p13-ter. This confirms the published assignment of this region to Ypl2-ter, but challenges the published assignment of Xpl4-ter and thus reorients the X chromosome physical map. BAC2O4, containing the X-linked microsatellite BM4604, mapped to the middle of the long arm of the X chromosome at q26-q31. The position of the physically mapped markers indicates either a lack of microsatellite markers for a large (30 to 50 cM) region of the short arm of the X chromosome or heterogeneity of recombination along the X chromosome. PMID:8833151

  12. Genetic and physical mapping of the bovine X chromosome

    Energy Technology Data Exchange (ETDEWEB)

    Yeh, Chen Chen; Taylor, J.F.; Sanders, J. O. [Texas A& M Univ., College Station, TX (United States)] [and others

    1996-03-01

    Three hundred eighty reciprocal backcross and F{sub 2} full sib progeny from 33 families produced by embryo transfer from 77 Angus (Bos taurus), Brahman (Bos indicus), and F{sub 1} parents and grandparents were used to construct genetic maps of the bovine X and Y chromosomes. All individuals were scored for 15 microsatellite loci, with an average of 608 informative meioses per locus. The length of the bovine X chromosome genetic map was 118.7 cM (female only) and of the pseudoautosomal region was 13.0 cM (male only). The 15-marker framework map in Kosambi centimorgans is (BM6017-6.1-TGLA89-35.8-TEXAN13-3.4-TGLA128-1.3-BM2713-21.1-BM4604-2.4-BR215-12.9-TGLA68-10.0-BM4321-1.0-HEL14-4.9-TGLA15-2.3-INRA120-12.5-TGLA325-1.6-MAF45-3.2-INRA30), with an average interval of 7.91 cM. Clones containing pseudoautosomal or sex-linked microsatellites were isolated from a bovine bacterial artificial chromosome library and were physically mapped to bovine metaphase chromosomes by fluorescence in situ hybridization to orient the X and Y chromosome maps. BAC57, containing the pseudoautosomal microsatellite INRA30, mapped to the distal end of the long arm of the X chromosome at q42-ter and to the short arm of the Y chromosome at p13-ter. This confirms the published assignment of this region to Yp12-ter, but challenges the published assignment of Xp14-ter and thus reorients the X chromosome physical map. BAC204, containing the X-linked microsatellite BM4604, mapped to the middle of the long arm of the X chromosome at q26-q31. The position of the physically mapped to the middle of the long arm of the X chromosome at q26-q31. The position of the physically mapped markers indicates either a lack of microsatellite markers for a large (30 to 50 cM) region of the short arm of the X chromosome or heterogeneity of recombination along the X chromosome. 46 refs., 2 figs., 3 tabs.

  13. Knowledge Management and Transfer

    Energy Technology Data Exchange (ETDEWEB)

    Sennanye, D.M.; Thugwane, S.J.; Rasweswe, M.A. [South African Young Nuclear Professionals Society, South African Nuclear Energy Cooperation, National Nuclear Regulator, P O Box 7106, Centurion 0046 (South Africa)

    2008-07-01

    Knowledge management has become an important concept in the nuclear industry globally. This has been driven by the fact that new reactors are commissioned and some are decommissioned. Since most old experts are near retirement then there is a need to capture the nuclear knowledge and expertise and transfer it to the new generation. Knowledge transfer is one of the important building blocks of knowledge management. Processes and strategies need to be developed in order to transfer this knowledge. South African Young Nuclear Professionals Society (SAYNPS) has established a document to address strategies that can be used to close the knowledge gap between the young less experienced and experts in the field. This action will help the young generation to participate in knowledge management. The major challenges will be the willingness of the experts to share and making sure that all knowledge is captured, stored and kept up to date. The paper presents the SAYNPS point of view with regard to knowledge transfer. (authors)

  14. Knowledge Management and Transfer

    International Nuclear Information System (INIS)

    Knowledge management has become an important concept in the nuclear industry globally. This has been driven by the fact that new reactors are commissioned and some are decommissioned. Since most old experts are near retirement then there is a need to capture the nuclear knowledge and expertise and transfer it to the new generation. Knowledge transfer is one of the important building blocks of knowledge management. Processes and strategies need to be developed in order to transfer this knowledge. South African Young Nuclear Professionals Society (SAYNPS) has established a document to address strategies that can be used to close the knowledge gap between the young less experienced and experts in the field. This action will help the young generation to participate in knowledge management. The major challenges will be the willingness of the experts to share and making sure that all knowledge is captured, stored and kept up to date. The paper presents the SAYNPS point of view with regard to knowledge transfer. (authors)

  15. Acción de la 5'azacitidina en cromosomas de células linfocitarias de bovinos 5'azacytidine effect in bovine lymphocytes chromosomes

    Directory of Open Access Journals (Sweden)

    Silvia Llambí

    2008-01-01

    Full Text Available La azacitidina (5-azaC es una sustancia que actúa inhibiendo la metilación del ADN, es utilizada en experiencias de clonación, transgénesis por transferencia nuclear y estudios de fragilidad cromosómica. Se ha observado la manifestación de diversas patologías en animales domésticos nacidos de experiencias de clonación. Ellas estarían asociadas a una inexacta reprogramación nuclear relacionada con fenómenos epigenéticos de metilación del ADN. En el presente trabajo se realizó una curva de inducción con diferentes concentraciones finales de 5-azaC (0 uM, 5 uM, 10 uM, 20 uM, 50 uM y 70 uM con el objetivo de estudiar los efectos sobre la estructura cromosómica en cultivos linfocitarios bovinos. Se utilizó la técnica de macrocultivo para análisis cromosómico a partir de sangre de un bovino macho (Bos taurus, Holstein Friesian. Se analizó un total de 50 placas metafásicas por tubo de cultivo y se encontraron diferencias significativas (pAzacytidine (5-azaC is a substance that inhibits the metilation of DNA, being used in cloning experiments, nuclear transference transgenesis and in chromosomal fragility studies. The manifestation of diverse pathologies has been observed in domestic animals born from cloning experiments. These would be associated to an incorrect nuclear reprogramation related to epigenetic metilation phenomena of the DNA. In the present work an induction curve with different final concentrations of 5-azaC (0 uM, 5 uM, 10 uM, 20 uM, 50 uM and 70 uM were carried out, with the objective of studying the effects on chromosome structure in bovine lymphocyte cultures. The macroculture technique was used for chromosomic analysis, from a blood sample of a male bovine (Bos taurus, Holstein Friesian. A total of 50 metaphases were analyzed from each culture. Significative differences (p<0.01 were found regarding the break points of chromosome X in the control culture with respect to the culture with higher concentration of 5

  16. 3-hydroxyflavone-bovine serum albumin interaction in Dextran medium

    Directory of Open Access Journals (Sweden)

    Voicescu Mariana

    2015-01-01

    Full Text Available Spectroscopic analysis of a bioactive flavonol, 3-Hydroxyflavone (3-HF, in systems based on Dextran 70 (Dx70 (an important bio-relevant polysacharide and Bovine Serum Albumin (BSA (a carrier protein, have been studied by fluorescence and circular dichroism. Changes produced by different concentrations of Dx70 on the fluorescent characteristics of 3-HF, and on the excited - state intramolecular proton transfer (ESIPT process were studied. The influence of 3-HF binding and of Dx70 on the secondary structure of BSA were investigated by circular dichroism spectroscopy. The influence of temperature (30-80°C range on the intrinsic Tryptophan fluorescence in 3-HF/BSA/Dx70 systems, was investigated. The results are discussed with relevance to 3-HF as a sensitive fluorescence probe for exploring flavone-protein interaction in plasma expander media and also for its biological evaluation.

  17. Oversight framework over oocyte procurement for somatic cell nuclear transfer: comparative analysis of the Hwang Woo Suk case under South Korean bioethics law and U.S. guidelines for human embryonic stem cell research.

    Science.gov (United States)

    Kim, Mi-Kyung

    2009-01-01

    We examine whether the current regulatory regime instituted in South Korea and the United States would have prevented Hwang's potential transgressions in oocyte procurement for somatic cell nuclear transfer, we compare the general aspects and oversight framework of the Bioethics and Biosafety Act in South Korea and the US National Academies' Guidelines for Human Embryonic Stem Cell Research, and apply the relevant provisions and recommendations to each transgression. We conclude that the Act would institute centralized oversight under governmental auspices while the Guidelines recommend politically-independent, decentralized oversight bodies including a special review body for human embryonic stem cell research at an institutional level and that the Guidelines would have provided more vigorous protection for the women who had undergone oocyte procurement for Hwang's research than the Act. We also suggest additional regulations to protect those who provide oocytes for research in South Korea.

  18. Interaction of Surface-active Fluorescence Probes with Bovine Serum Albumin

    Institute of Scientific and Technical Information of China (English)

    Tong Kuan XU; Xing Hai SHEN; Na LI; Hong Cheng GAO

    2005-01-01

    The binding between three surface-active substituted 3H-indole fluorescence probes and bovine serum albumin (BSA) in aqueous solution was studied using fluorescence quenching. The binding constants of 3H-indole molecules with BSA were obtained. According to the Forster resonance energy transfer theory, the distances between 3H-indole molecules and tryptophan of BSA were calculated. The results show that the oligoethyloxyethylene chain of 3H-indole molecules is longer, the binding between them is stronger, the energy transfer efficiency is higher,and the distance between tryptophan and 3H-indole is nearer.

  19. Electron transfer complex between nitrous oxide reductase and cytochrome c552 from Pseudomonas nautica: kinetic, nuclear magnetic resonance, and docking studies

    OpenAIRE

    Moura, Isabel; Moura, José J. G.; Pauleta, Sofia R.; Monzani, Enrico; Pereira, Alice S; Casella, Luigi; Dell’Acqua, Simone

    2008-01-01

    The multicopper enzyme nitrous oxide reductase (N 2OR) catalyzes the final step of denitrification, the two-electron reduction of N 2O to N 2. This enzyme is a functional homodimer containing two different multicopper sites: CuA and CuZ. CuA is a binuclear copper site that transfers electrons to the tetranuclear copper sulfide CuZ, the catalytic site. In this study, Pseudomonas nautica cytochrome c 552 was identified as the physiological electron donor. The kinetic data show differences when ...

  20. Bovine colostrum: an emerging nutraceutical.

    Science.gov (United States)

    Bagwe, Siddhi; Tharappel, Leo J P; Kaur, Ginpreet; Buttar, Harpal S

    2015-09-01

    Nutraceutical, a term combining the words "nutrition" and "pharmaceuticals", is a food or food product that provides health benefits as an adjuvant or alternative therapy, including the treatment and prevention of infectious diseases in children and adults. There is emerging evidence that bovine colostrum (BC) may be one of the promising nutraceuticals which can prevent or mitigate various diseases in newborns and adults. Immunity-related disorders are one of the leading causes of mortality in the world. BC is rich in immunity, growth and antimicrobial factors, which promote tissue growth and the maturation of digestive tract and immune function in neonatal animals and humans. The immunoglobulins and lactoferrin present in colostrum are known to build natural immunity in newborns which helps to reduce the mortality rate in this population. Also, the side-effect profile of colostrum proteins and possible lactose intolerance is relatively less in comparison with milk. In general, BC is considered safe and well tolerated. Since colostrum has several important nutritional constituents, well-designed, double-blind, placebo-controlled studies with colostrum products should be conducted to widen its therapeutic use. The objectives of this review are to create awareness about the nutraceutical properties of colostrum and to discuss the various ongoing alternative treatments of colostrum and its active ingredients as well as to address colostrum's future nutraceutical and therapeutic implications in humans. PMID:25781716