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Sample records for boto inia geoffrensis

  1. Presumed normal ultrasonographic findings of the testis and epididymis of botos (Inia geoffrensis).

    Science.gov (United States)

    Alves, Flávio Ribeiro; da Silva, Vera Maria Ferreira; Martin, Anthony Richard; Ambrósio, Carlos Eduardo; Giglio, Robson Fortes; Miglino, Maria Angélica

    2012-12-01

    Fifteen live adult male botos, or Amazon river dolphins (Inia geoffrensis), were examined using ultrasonography during the yearly capture expedition, between October and November 2005, at the Mamirauá Sustainable Development Reserve, within the Brazilian Amazon (3 degrees S, 65 degrees W). All examinations were performed with a Sonosite 180 plus ultrasound unit in conjunction with a 2- to 5-MHz multifrequency transducer convex array 180 Plus/Elite-C60. Age and maturity estimates were determined considering the body length, weight, and external characteristics. In all examinations, the testes were discerned by the presence of a hyperechoic central line, called the mediastinum testis, a landmark for their identification during ultrasonography. No significant differences in echogenicity were detected on the ultrasonographic appearance of the testes among the studied animals. On adult male botos, apparent parenchymal nodulation of the testis was observed on scanning in most of the animals and probably constituted evidence of reproductive maturity. Using the color Doppler technique, blood flow was detected along the mediastinum testis that progressively decreased toward the periphery of this organ. Little blood flow could be identified by color Doppler. Power Doppler allowed better accuracy to identify testicular vessels, their topography, and their differentiation from adjacent structures. Ultrasonographic examination provides useful data for morphologic characterization of the boto's testes. Examination using Doppler techniques was considered a valuable tool to evidence blood flow through the testicular parenchyma.

  2. Hematologia e bioquímica sangüínea do boto Vermelho Inia Geoffrensis (de Blainville, 1817)

    OpenAIRE

    Mello, Daniela Magalhães Drummond de

    2006-01-01

    Os golfinhos de rio estão entre os cetáceos menos conhecidos e mais ameaçados do mundo. O boto vermelho (Inia geoffrensis) é endêmico e amplamente distribuído nas bacias dos rios Amazonas e Orinoco. Visando aumentar conhecimento sobre a biologia básica e fisiologia do mesmo, o presente trabalho reporta os parâmetros hematológicos e bioquímicos de 109 botos de vida livre da Reserva de Desenvolvimento Sustentável Mamirauá. As colheitas foram realizadas durante o mês de novembro de 2004 e 2005. ...

  3. Acoustic characteristics of biossonar sounds of free-ranging botos (Inia geoffrensis) and tucuxis (Sotalia fluviatilis) in the Negro River, Amazon, Brazil

    OpenAIRE

    Yukiko Yamamoto; Tomonari Akamatso; Vera Maria Ferreira da Silva; Yayoi Yoshida; Shiro Kohshima

    2015-01-01

    Odontoceti emit broadband high-frequency clicks on echolocation for orientation or prey detection. In the Amazon Basin, two odontoceti species, boto (Amazon River dolphin, Inia geoffrensis) and tucuxi (Sotalia fluviatilis), live sympatrically. The acoustic characteristics of the echolocation clicks of free-ranging botos and tucuxis were measured with a hydrophone array consisting of a full-band and an acoustic event recorder (A-tag). The clicks of the two species were short-duration broadband...

  4. Isolamento e Caracterização de Marcadores Microssatélites para análise de Parentesco no Boto-vermelho (Inia geoffrensis)

    OpenAIRE

    Gravena, Waleska

    2006-01-01

    O presente trabalho foi dividido em dois capítulos: no capítulo I foi realizado tanto o isolamento e caracterização de marcadores microssatélites específicos para o boto-vermelho (Inia geoffrensis), como caracterização de marcadores desenvolvidos para outras espécies de cetáceos. Estes marcadores também foram caracterizados para a franciscana (Pontoporia blainvillei) e para o boto-cinza (Sotalia guianensis). No capítulo II foram feitas análises para determinação de relacionamentos dentro e en...

  5. As atividades turísticas baseadas na alimentação artificial de botos-da-Amazônia (Inia geoffrensis) e a legislação ambiental brasileira

    OpenAIRE

    Luiz Cláudio Pinto de Sá Alves; Carlos José Saldanha Machado; Rodrigo Machado Vilani; Marcelo Derzi Vidal; Artur Andriolo; Alexandre de Freitas Azevedo

    2013-01-01

    Here we evaluate the touristic activity involving the interaction between botos (Inia geoffrensis) and humans based on the conditioning through artificial feeding in the light of the environmental legislation, using the Anavilhanas National Park (ANP) as case study. The Park is located in the Brazilian Amazon, in Novo Airão city, Amazonas State. This activity has significantly increased over the last years, as well as the literature describing its negative effects. By consulting the specializ...

  6. As atividades turísticas baseadas na alimentação artificial de botos-da-Amazônia (Inia geoffrensis) e a legislação ambiental brasileira

    OpenAIRE

    Alves,Luiz Cláudio Pinto de Sá; Machado, Carlos José Saldanha; Vilani, Rodrigo Machado; Vidal, Marcelo Derzi; Andriolo, Artur; Azevedo, Alexandre de Freitas

    2013-01-01

    A atividade turística de interação ser humano-boto-da-Amazônia (Inia geoffrensis), baseada na prática do condicionamento por meio da alimentação artificial, vem aumentando quantitativamente nos últimos anos, assim como a literatura que descreve os seus efeitos negativos. O objetivo deste trabalho é avaliar essa atividade à luz da legislação ambiental, tomando como estudo de caso o Parque Nacional de Anavilhanas (PNA), localizado em Novo Airão, Estado do Amazonas, Brasil. A partir de consultas...

  7. As atividades turísticas baseadas na alimentação artificial de botos-da-Amazônia (Inia geoffrensis e a legislação ambiental brasileira

    Directory of Open Access Journals (Sweden)

    Luiz Cláudio Pinto de Sá Alves

    2013-12-01

    Full Text Available Here we evaluate the touristic activity involving the interaction between botos (Inia geoffrensis and humans based on the conditioning through artificial feeding in the light of the environmental legislation, using the Anavilhanas National Park (ANP as case study. The Park is located in the Brazilian Amazon, in Novo Airão city, Amazonas State. This activity has significantly increased over the last years, as well as the literature describing its negative effects. By consulting the specialized literature, analyzing the legal framework and empirical material, it was verified that i legally speaking this practice presents conflicts to some aspects of the Brazilian environmental legislation, ecotourism concepts and functions of the Conservation Units and, ii administratively speaking, the environmental agencies are now taking appropriate steps to mitigate the negative impacts and gradual actions in order to alter the touristic activities to comply with the current environmental legislation. We conclude that there is still a lot to be done for the botos tourism model that occurs in the Brazilian Amazon to be conducted in a sustainable manner, suggesting that the group of heterogeneous actions proposed here be implemented to achieve that.

  8. Microsatellite loci for population and parentage analysis in the Amazon River dolphin (Inia geoffrensis de Blainville, 1817).

    Science.gov (United States)

    Gravena, Waleska; Hrbek, Tomas; DA Silva, Vera M S; Astolfi-Filho, Spartaco; Farias, Izeni P

    2009-03-01

    We developed specific primers for microsatellite DNA regions for the Amazon River dolphin or boto Inia geoffrensis, for use in population and conservation genetic studies. We also tested their transferability for two other species, Pontoporia blainvillei (sister taxon of I. geoffrensis) and Sotalia guianensis. A total of 12 microsatellite loci were polymorphic for the boto. An additional 25 microsatellite loci previously isolated from other cetacean species were also tested in the boto. The 26 polymorphic microsatellite loci indicate they will be excellent markers for studies of population structure and kinship relations of the boto.

  9. Spatial distribution of river dolphins, Inia geoffrensis (Iniidae), in the Araguaia River (central Brazil)

    OpenAIRE

    ARAÚJO, CLARYANA COSTA; Vera Maria Ferreira da Silva

    2014-01-01

    This study aimed to characterize the distribution of botos, Inia geoffrensis, along a 530 km stretch in the middle reaches of the Araguaia River (central Brazil). Data collection was conducted in May (lowering water season) and September (dry season) of 2009. The location and group size of botos were recorded, and the relative density was calculated. The river was divided into nine areas according to geomorphological features; these areas were later grouped into two (low and high) levels of s...

  10. Molecular cytogenetic characterization of the Amazon River dolphin Inia geoffrensis.

    Science.gov (United States)

    Bonifácio, Heidi L; da Silva, Vera M F; Martin, Anthony R; Feldberg, Eliana

    2012-09-01

    Classical and molecular cytogenetic (18S rDNA, telomeric sequence, and LINE-1 retrotransposon probes) studies were carried out to contribute to an understanding of the organization of repeated DNA elements in the Amazon River dolphin (boto, Inia geoffrensis). Twenty-seven specimens were examined, each presenting 2n = 44 chromosomes, the karyotype formula 12m + 14sm + 6st + 10t + XX/XY, and fundamental number (FN) = 74. C-positive heterochromatin was observed in terminal and interstitial positions, with the occurrence of polymorphism. Interstitial telomeric sequences were not observed. The nucleolar organizer region (NOR) was located at a single site on a smallest autosomal pair. LINE-1 was preferentially distributed in the euchromatin regions, with the greatest accumulation on the X chromosome. Although the karyotype structure in cetaceans is considered to be conserved, the boto karyotype demonstrated significant variations in its formula, heterochromatin distribution, and the location of the NOR compared to other cetacean species. These results contribute to knowledge of the chromosome organization in boto and to a better understanding of karyoevolution in cetaceans.

  11. Percepção de turistas sobre “atividade/interação” com botos vermelhos (Inia geoffrensis (de Blainville, 1817) no estado do Amazonas, Brasil

    OpenAIRE

    Frias, Mariana Paschoalini

    2014-01-01

    Muito têm se falado sobre a problemática envolvendo turistas e botos vermelhos na Amazônia. A interação boto-homem com fins turísticos no Brasil é relatada há aproximadamente seis anos por pesquisadores que observaram efeitos negativos desse tipo de atividade tanto para animais (mudanças comportamentais, principalmente) quanto para turistas envolvidos (acidentes com lesões). Porém, a forma menos impactante dessa atividade, o Ecoturismo, é apontada por muitos pesquisadores a nível nacional e i...

  12. Unihemispheric slow-wave sleep in the Amazonian dolphin, Inia geoffrensis.

    Science.gov (United States)

    Mukhametov, L M

    1987-08-18

    An electroencephalographic study of sleep in Amazonian dolphins, Inia geoffrensis, revealed that unihemispheric slow-wave sleep is the dominant sleep type in this species, as in the other two dolphin species that were studied earlier.

  13. Demographische Untersuchungen am Amazonasdelfin (Inia geoffrensis) im Cuyabeno-Reservat, in Ecuador

    OpenAIRE

    Denkinger, Judith

    2001-01-01

    Amazonasdelfine (Inia geoffrensis) sind im gesamten Amazonas- und Orinocobecken verbreitet. In Ecuador kommen sie in den Flusssystemen des Pastaza, Curaray, Napo und Aguarico vor. Bisher gibt es nur sehr wenig Informationen über die Populationsdynamik, die Habitatnutzung oder das Sozialsystem von Inia, auch fehlen standardisierte Methoden, um beispielsweise Populationsschätzungen aus verschiedenen Studiengebieten miteinander vergleichen zu können. Darüber hinaus sind Flussdelfine durch die Ze...

  14. Electrophysiological study of interaural sound intensity difference in the dolphin Inia geoffrensis.

    Science.gov (United States)

    Supin AYa; Popov, V V; Klishin, V O

    1991-09-15

    A wave observed in the auditory brainstem responses (ABR), sensitive to the side of sound presentation, is described in a dolphin (Inia geoffrensis). Dependence of the wave threshold on location of the sound source showed that the interaural intensity difference was more than 20 dB.

  15. A third report of "golf ball disease" in an Amazon River dolphin (Inia geoffrensis) associated with Streptococcus iniae.

    Science.gov (United States)

    Bonar, Christopher J; Wagner, Robert A

    2003-09-01

    An Amazon River dolphin (Inia geoffrensis) developed a dermatologic syndrome characterized by the occurrence of slow-growing, nodular, s.c. abscesses. Initial biopsies, cultures, and cytologic analysis of needle aspirates from the abscesses indicated steatitis with probable secondary, gram-negative bacterial infection. Treatment with dietary vitamin E supplement and broad-spectrum antibiotics yielded minimal improvement. Subsequent cultures revealed Streptococcus iniae in addition to several gram-negative bacteria. Vigorous surgical management of the abscesses, including lancing, debridement, and irrigation, combined with antimicrobial therapy specific for Streptococcus and gram-negative organisms, and improvement of the animal's diet and environmental water quality led to gradual recovery. When the animal was ill, it demonstrated an inflammatory leukogram and transient uremia. Streptococcus iniae is a serious pathogen of aquacultured fishes and humans and should be included in the differential diagnosis of chronic dermatopathy in river dolphins. Specific antimicrobial therapy, excellent water quality, surgical management of abscesses, and adherence to sanitary protocols should be observed in cases of suspected S. iniae infection in dolphins.

  16. Stomach Content of a Juvenile Bolivian River Dolphin (Inia geoffrensis boliviensis) from the Upper Madeira Basin, Bolivia

    NARCIS (Netherlands)

    Aliaga-Rossel, E.; Beerman, A.S.; Sarmiento, J.

    2010-01-01

    The article presents a study about the stomach content of a juvenile Bolivian river dolphin (Inia geoffrensis boliviensis), an endemic subspecies of the Amazon River dolphin, found in the upper Madeira River basin in Bolivia. The study finds that the stomach of Bolivian river dolphin contained a mix

  17. Seroprevalence of Toxoplasma gondii in free-living Amazon river dolphins (Inia geoffrensis) from central Amazon, Brazil

    Science.gov (United States)

    Toxoplasma gondii is an important pathogen in aquatic mammals and its presence in these animals may indicate water contamination of aquatic environment by oocysts. Serum samples from 95 dolphins from free-living Amazon River dolphins (Inia geoffrensis) from Sustainable Development Reserve Mamirauá (...

  18. Seroprevalence of Toxoplasma gondii in free-living Amazon River dolphins (Inia geoffrensis) from central Amazon, Brazil

    OpenAIRE

    P.S. Santos; ALBUQUERQUE, G. R.; da Silva, V. M. F.; Martin, A R; Marvulo,M.F.V.; S.L.P. Souza; Ragozo, A. M. A. [UNESP; Nascimento, C. C.; Gennari,S.M.; Dubey, J. P.; Silva, J. C. R.

    2011-01-01

    Toxoplasma gondii is an important pathogen in aquatic mammals and its presence in these animals may indicate the water contamination of aquatic environment by oocysts. Serum samples from 95 free-living Amazon River dolphins (Inia geoffrensis) from the Mamiraua Sustainable Development Reserve (RDSM), Tefe, Amazonas, Central Amazon, Brazil were tested for T. gondii antibodies using the modified agglutination test (MAT). Antibodies (MAT >= 25) to T. gondii were found in 82 (86.3%) dolphins with ...

  19. The complete amino acid sequence of the major component myoglobin of Amazon river dolphin (Inia geoffrensis).

    Science.gov (United States)

    Dwulet, F E; Bogardt, R A; Jones, B N; Lehman, L D; Gurd, F R

    1975-12-02

    The complete amino acid sequence of the major component myoglobin from Amazon River dolphin, Inia geoffrensis, was determined by specific cleavage of the protein to obtain large peptides which are readily degraded by the automatic sequencer. Three easily separable peptides were obtained by cleaving the protein with cyanogen bromide at the methionine residues and four peptides were obtained by cleaving the methyl-acetimidated protein with trypsin at the arginine residues. From these peptides over 85% of the sequence was completed. The remainder of the sequence was obtained by fragmentation of the large cyanogen bromide peptide with trypsin. This protein differs from that of the common porpoise, Phocoena phocoena, at seven positions, from that of the common dolphin, Delphinus delphis, at 11 positions, and from that of the sperm whale, Physeter catodon, at 15 positions. By comparison of this sequence with the three-dimensional structure of sperm whale myoglobin it appears that those residues close to the heme group are most conserved followed by those in nonhelical regions and lastly by those in the helical segments. All of the substitutions observed in this sequence fit easily into the three-dimensional structure of the sperm whale myoglobin.

  20. Filogeografia e gen?tica de popula??es de inia geoffrensis (cetartiodactyla: iniidae) nos rios Negro e Branco e evid?ncia de linhagem evolutiva independente na Bacia do Orinoco

    OpenAIRE

    Farias, Joiciane Gon?alves

    2015-01-01

    Os golfinhos de ?gua doce do g?nero Inia pertencem a fam?lia Iniidae e possuem tr?s esp?cies descritas para diferentes regi?es hidrogr?ficas: Inia boliviensis (sub-bacia Boliviana); I. araguaiaensis (bacia Tocantins/Araguaia); e I. geoffrensis (bacias Amaz?nica e do Orinoco). Esta ?ltima ? representada por duas subesp?cies, cuja taxonomia ? controversa: Inia geoffrensis geoffrensis (bacia Amaz?nia) e I. g. humboldtiana (bacia do Orinoco). As adapta??es adquiridas pelas esp?cies...

  1. Seroprevalence of Toxoplasma gondii in free-living Amazon River dolphins (Inia geoffrensis) from central Amazon, Brazil.

    Science.gov (United States)

    Santos, P S; Albuquerque, G R; da Silva, V M F; Martin, A R; Marvulo, M F V; Souza, S L P; Ragozo, A M A; Nascimento, C C; Gennari, S M; Dubey, J P; Silva, J C R

    2011-12-29

    Toxoplasma gondii is an important pathogen in aquatic mammals and its presence in these animals may indicate the water contamination of aquatic environment by oocysts. Serum samples from 95 free-living Amazon River dolphins (Inia geoffrensis) from the Mamirauá Sustainable Development Reserve (RDSM), Tefé, Amazonas, Central Amazon, Brazil were tested for T. gondii antibodies using the modified agglutination test (MAT). Antibodies (MAT ≥ 25) to T. gondii were found in 82 (86.3%) dolphins with titers of 1:25 in 24, 1:50 in 56, and 1:500 in 2. Results suggest a high level contamination of the aquatic environment of the home range of these animals.

  2. A retrospective study of pathologic findings in the Amazon and Orinoco river dolphin (Inia geoffrensis) in captivity.

    Science.gov (United States)

    Bonar, Christopher J; Boede, Ernesto O; Hartmann, Manuel García; Lowenstein-Whaley, Joanne; Mujica-Jorquera, Esmeralda; Parish, Scott V; Parish, James V; Garner, Michael M; Stadler, Cynthia K

    2007-06-01

    River dolphins are especially susceptible to negative human impacts. For their conservation, attempts of relocation or procreation ex situ may become important in the future to avoid their extinction. Additional knowledge and medical experiences of river dolphin management in captivity may aid such conservation efforts. The medical records and necropsy and histopathology reports on 123 captive Amazon River dolphins (Inia geoffrensis) were re-viewed. Of these 123 animals, 105 were necropsied and 70 necropsies were supported with histopathology. Eighteen animals were not necropsied. Among wild-born animals, mortality was highest in the first 2 mo immediately postcapture and transport, accounting for 32 of 123 deaths. Pneumonia and skin lesions (cutaneous and subcutaneous ulcerations and abscesses) were the most common findings, found in 44 of 105 (42%) and 38 of 105 (36%) of gross diagnoses, respectively. At least 10 of 44 cases of pneumonia diagnosed grossly included a verminous component. Cachexia, from a variety of causes, was a major gross finding in 21 animals. Fifteen animals had histologic evidence of significant renal pathology, and this was the primary cause of death in 13 cases. Hepatic pathology was found in 18 cases, and bacterial sepsis was confirmed via histology in 16 cases. Based on these findings, it may be concluded that keys to successful maintenance of this species include 1) prophylactic anthelminthic and antibiotic therapy immediately post-capture; 2) maintenance of animals in larger enclosures than in past attempts, in compatible groups, and in facilities capable of separating aggressive animals; 3) maintenance in microbiologically hygienic water quality at all times; and 4) a proactive program of preventive medicine during the immediate postcapture, quarantine, and maintenance period of captivity.

  3. Placentation in dolphins from the Amazon River Basin: the Boto, Inia geoffrensis, and the Tucuxi, Sotalia fluviatilis

    OpenAIRE

    Bonatelli Marina; Carvalho Ana F; Ambrosio Carlos E; Carter Anthony M; da Silva Vera MF; Lima Marcelo C; Miglino Maria

    2007-01-01

    Abstract A recent reassessment of the phylogenetic affinities of cetaceans makes it timely to compare their placentation with that of the artiodactyls. We studied the placentae of two sympatric species of dolphin from the Amazon River Basin, representing two distinct families. The umbilical cord branched to supply a bilobed allantoic sac. Small blood vessels and smooth muscle bundles were found within the stroma of the cord. Foci of squamous metaplasia occurred in the allanto-amnion and allan...

  4. Placentation in dolphins from the Amazon River Basin: the Boto, Inia geoffrensis, and the Tucuxi, Sotalia fluviatilis.

    Science.gov (United States)

    da Silva, Vera M F; Carter, Anthony M; Ambrosio, Carlos E; Carvalho, Ana F; Bonatelli, Marina; Lima, Marcelo C; Miglino, Maria Angelica

    2007-06-28

    A recent reassessment of the phylogenetic affinities of cetaceans makes it timely to compare their placentation with that of the artiodactyls. We studied the placentae of two sympatric species of dolphin from the Amazon River Basin, representing two distinct families. The umbilical cord branched to supply a bilobed allantoic sac. Small blood vessels and smooth muscle bundles were found within the stroma of the cord. Foci of squamous metaplasia occurred in the allanto-amnion and allantochorion. The interhemal membrane of the placenta was of the epitheliochorial type. Two different types of trophoblastic epithelium were seen. Most was of the simple columnar type and indented by fetal capillaries. However, there were also areolar regions with tall columnar trophoblast and these were more sparsely supplied with capillaries. The endometrium was well vascularised and richly supplied with actively secreting glands. These findings are consistent with the current view that Cetacea are nested within Artiodactyla as sister group to the hippopotamids.

  5. Placentation in dolphins from the Amazon River Basin: the Boto, Inia geoffrensis, and the Tucuxi, Sotalia fluviatilis

    Directory of Open Access Journals (Sweden)

    Bonatelli Marina

    2007-06-01

    Full Text Available Abstract A recent reassessment of the phylogenetic affinities of cetaceans makes it timely to compare their placentation with that of the artiodactyls. We studied the placentae of two sympatric species of dolphin from the Amazon River Basin, representing two distinct families. The umbilical cord branched to supply a bilobed allantoic sac. Small blood vessels and smooth muscle bundles were found within the stroma of the cord. Foci of squamous metaplasia occurred in the allanto-amnion and allantochorion. The interhemal membrane of the placenta was of the epitheliochorial type. Two different types of trophoblastic epithelium were seen. Most was of the simple columnar type and indented by fetal capillaries. However, there were also areolar regions with tall columnar trophoblast and these were more sparsely supplied with capillaries. The endometrium was well vascularised and richly supplied with actively secreting glands. These findings are consistent with the current view that Cetacea are nested within Artiodactyla as sister group to the hippopotamids.

  6. First report of major histocompatibility complex class II loci from the Amazon pink river dolphin (genus Inia).

    Science.gov (United States)

    Martínez-Agüero, M; Flores-Ramírez, S; Ruiz-García, M

    2006-07-31

    We report the first major histocompatibility complex (MHC) DQB1 sequences for the two species of pink river dolphins (Inia geoffrensis and Inia boliviensis) inhabiting the Amazon and Orinoco River basins. These sequences were found to be polymorphic within the Inia genus and showed shared homology with cetacean DQB-1 sequences, especially, those of the Monodontidae and Phocoenidae. On the other hand, these sequences were shown to be divergent from those described for other riverine dolphin species, such as Lipotes vexillifer, the Chinese river dolphin. Two main conclusions can be drawn from our results: 1) the Mhc DQB1 sequences seem to evolve more rapidly than other nuclear sequences in cetaceans, and 2) differential positive selective pressures acting on these genes cause concomitant divergent evolutionary histories that derive phylogenetic reconstructions that could be inconsistent with widely accepted intertaxa evolutionary relationships elucidated with other molecular markers subjected to a neutral dynamics.

  7. Streptococcus iniae and Streptococcus agalactiae

    Science.gov (United States)

    Streptococcus iniae and S. agalactiae are economically important Gram positive bacterial pathogens of cultured and wild fish with a worldwide distribution. Both bacteria are potential zoonotic pathogens and have been associated most often with infections in immunocompromised people. Streptococcus in...

  8. Amazon river dolphins (Inia geoffrensis) use a high-frequency short-range biosonar.

    Science.gov (United States)

    Ladegaard, Michael; Jensen, Frants Havmand; de Freitas, Mafalda; Ferreira da Silva, Vera Maria; Madsen, Peter Teglberg

    2015-10-01

    Toothed whales produce echolocation clicks with source parameters related to body size; however, it may be equally important to consider the influence of habitat, as suggested by studies on echolocating bats. A few toothed whale species have fully adapted to river systems, where sonar operation is likely to result in higher clutter and reverberation levels than those experienced by most toothed whales at sea because of the shallow water and dense vegetation. To test the hypothesis that habitat shapes the evolution of toothed whale biosonar parameters by promoting simpler auditory scenes to interpret in acoustically complex habitats, echolocation clicks of wild Amazon river dolphins were recorded using a vertical seven-hydrophone array. We identified 404 on-axis biosonar clicks having a mean SLpp of 190.3 ± 6.1 dB re. 1 µPa, mean SLEFD of 132.1 ± 6.0 dB re. 1 µPa(2)s, mean Fc of 101.2 ± 10.5 kHz, mean BWRMS of 29.3 ± 4.3 kHz and mean ICI of 35.1 ± 17.9 ms. Piston fit modelling resulted in an estimated half-power beamwidth of 10.2 deg (95% CI: 9.6-10.5 deg) and directivity index of 25.2 dB (95% CI: 24.9-25.7 dB). These results support the hypothesis that river-dwelling toothed whales operate their biosonars at lower amplitude and higher sampling rates than similar-sized marine species without sacrificing high directivity, in order to provide high update rates in acoustically complex habitats and simplify auditory scenes through reduced clutter and reverberation levels. We conclude that habitat, along with body size, is an important evolutionary driver of source parameters in toothed whale biosonars.

  9. Notes on the nasal tract complex of the Boutu, Inia geoffrensis (De Blainville, 1817) (Cetacea, Platanistidae)

    NARCIS (Netherlands)

    Schenkkan, E.J.

    1977-01-01

    The anatomy of the nasal passage of three specimens of the Boutu has been studied. The various diverticula appear to be aberrant from those described for other Platanistidae. The vestibular airsac system has developed into one enormous diverticulum. Except for a narrow region caudal to the blowhole,

  10. Amazon river dolphins (Inia geoffrensis) use a high-frequency short-range biosonar

    DEFF Research Database (Denmark)

    Ladegaard, Michael; Jensen, Frants Havmand; de Freitas, Mafalda;

    2015-01-01

    Toothed whales produce echolocation clicks with source parameters related to body size; however, it may be equally important to consider the influence of habitat, as suggested by studies on echolocating bats. A few toothed whale species have fully adapted to river systems, where sonar operation...

  11. Amazon river dolphins (Inia geoffrensis) use a high-frequency short-range biosonar

    OpenAIRE

    Michael Ledegaard; Frants Havmand Jensen; Mafalda de Freitas; Vera Maria Ferreira da Silva; Peter Teglberg Madsen

    2015-01-01

    Toothed whales produce echolocation clicks with source parameters related to body size; however, it may be equally important to consider the influence of habitat, as suggested by studies on echolocating bats. A few toothed whale species have fully adapted to river systems, where sonar operation is likely to result in higher clutter and reverberation levels than those experienced by most toothed whales at sea because of the shallow water and dense vegetation. To test the hypothesis that habita...

  12. Perception of local inhabitants regarding the socioeconomic impact of tourism focused on provisioning wild dolphins in Novo Airão, Central Amazon, Brazil.

    Science.gov (United States)

    Alves, Luiz C P S; Zappes, Camilah A; Oliveira, Rafael G; Andriolo, Artur; Azevedo, Alexandre de F

    2013-01-01

    Botos (Inia geoffrensis) are currently provisioned for use in tourist attractions in five sites in the Brazilian Amazon. Despite the known negative effects associated with human-wild dolphin interactions, this activity has been regulated and licensed in the Anavilhanas National Park in Novo Airão, Amazonas State, Brazil. We present an updated evaluation of the perception of the local community concerning the possible socioeconomic impacts of this tourism in Novo Airão. In April 2011, 45 interviews were conducted with inhabitants. A small segment of Novo Airão perceives currently itself as being economically dependent on the botos feeding tourism. Despite that, the economic benefits of this controversial activity apparently are not shared among most inhabitants, and botos feeding tourism is perceived as generating diverse negative effects. We conclude that if the activity was banned or modified into a less impacting tourist activity, this action would probably not majorly affect the lives of the general population.

  13. Complete Genome Sequence of Streptococcus iniae 89353, a Virulent Strain Isolated from Diseased Tilapia in Taiwan

    Science.gov (United States)

    Wu, Sheng-Han; Chen, Chun-Yao; Huang, Chang-Wen; Lu, Jenn-Kan; Chou, Hsin-Yiu

    2017-01-01

    ABSTRACT Streptococcus iniae 89353 is a virulent strain isolated from diseased tilapia in Taiwan. The full-genome sequence of S. iniae 89353 is 2,098,647 bp. The revealed genome information will be beneficial for identification and understanding of potential virulence genes of Streptococcus iniae and possible immunogens for vaccine development against streptococcosis. PMID:28126946

  14. [A microbiological examination of the skin and vaginal mucus of Amazon River freshwater dolphins (Inia geoffrensis) in relation to an assessment of the physiological state of the animals].

    Science.gov (United States)

    Ushakova, N A

    1991-01-01

    There is a definite relation between the state of dolphin's health and the abundance of bacterial associations developing on the skin surface around the dorsal fin. The disease was accompanied by an increase in bacterium amount more than fourfold due to the enhancement of staphylococcus and pseudomonad number. The development of dystrophy in the animal resulted in a threefold decrease in bacterium number. Pseudomonas aeruginosa dominated in this case. In the vaginal mucose of female, the development of bacteria had an oscillatory character and was correlated with the dynamics of leukocyte number against the background of morphologically diverse epithelial cells discharged in different periods of observation. Periods of the abundant bacterium development were connected with the cyclic recurrence of sexual (estrous) processes in the female organism.

  15. Immunoproteomic analysis of the antibody response obtained in tilapia following immunization with a Streptococcus iniae vaccine

    Science.gov (United States)

    Streptococcus iniae is one of the most economically important Gram-positive pathogens in cultured fish species worldwide. Research has shown that vaccination is a tool that can be used in the prevention of streptococcal disease. The USDA-ARS patented S. iniae vaccine has been demonstrated to be ef...

  16. Controlled laboratory challenge demonstrates substantial additive genetic variation in resistance to Streptococcus iniae in Nile tilapia

    Science.gov (United States)

    Streptococcus iniae is an etiologic agent of streptococcal disease in tilapia and is one of several Streptococcus spp. that negatively impact worldwide tilapia production. Methods for the prevention and control of S. iniae include vaccines, management strategies, and antibiotics. An alternative and ...

  17. Protective efficiency of an inactivated vaccine against Streptococcus iniae in olive flounder, Paralichthys olivaceus

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    Jeong Yong-Uk

    2016-03-01

    Full Text Available Streptococcus iniae is a causative agent of hemorrhagic septicemia in olive flounder, Paralichthys olivaceus, in Korea, resulting in serious economic losses. As a preventive measure, M VAC INIAE (Mastuken, Japan was prepared from the S. iniae F2K strain and tested against the SI-36 strain prevalent on flounder fish farms on Jeju Island, Korea. F2K had a serotype of 38 (− and SI-36 38 (+. The vaccine recognized both serotypes. It showed a very high effective immune response against S. iniae; the challenge test using the S. iniae SI-36 strain resulted in a relative percent survival (RPS of 85.7-87.0% 2 weeks after vaccination and 71.0-80.0% 6 months after vaccination. Field vaccination and clinical challenge tests were performed at local Jeju aquafarms with S. iniae SI-36. These showed significantly reduced cumulative mortality when compared to the control group with RPS rates that ranged between 71-80%. Hence, the present study suggests that this vaccine showed a significant immune response against S. iniae and could be applied in commercial aquafarms as a therapeutic agent against β-hemolytic streptococcosis in cultured P. olivaceus.

  18. Auditory Evoked Potentials for the Evaluation of Hearing Sensitivity in Navy Dolphins. Modification P00002: Assessment of Hearing Sensitivity in Adult Male Elephant Seals

    Science.gov (United States)

    2006-12-30

    investigation of Finneran, J. J., Schlundt, C. E., Carder, D. A., and Ridgway, S. H. (2002). hearing in the freshwater dolphin Inia geoffrensis ," Dokl. Biol. Sci...2006 Acoustical Society of America 3181 taliafluviatilis; Popov and Supin, 1990a), Amazon river dol- Computer -s- phins ( Inia geoffrensis ; Popov and...simultaneously gated pure tones," J. of hearing in fresh water dolphin Inia Geoffrensis ." Dokl. Biol. Sci. Acoust. Soc. Am., 94, 3215-3226. 313(1), 238-241

  19. Perception of local inhabitants regarding the socioeconomic impact of tourism focused on provisioning wild dolphins in Novo Airao, Central Amazon, Brazil

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    LUIZ C.P.S. ALVES

    2013-11-01

    Full Text Available Botos (Inia geoffrensis are currently provisioned for use in tourist attractions in five sites in the Brazilian Amazon. Despite the known negative effects associated with human-wild dolphin interactions, this activity has been regulated and licensed in the Anavilhanas National Park in Novo Airão, Amazonas State, Brazil. We present an updated evaluation of the perception of the local community concerning the possible socioeconomic impacts of this tourism in Novo Airão. In April 2011, 45 interviews were conducted with inhabitants. A small segment of Novo Airão perceives currently itself as being economically dependent on the botos feeding tourism. Despite that, the economic benefits of this controversial activity apparently are not shared among most inhabitants, and botos feeding tourism is perceived as generating diverse negative effects. We conclude that if the activity was banned or modified into a less impacting tourist activity, this action would probably not majorly affect the lives of the general population.

  20. Conflicts between river dolphins (Cetacea: Odontoceti and fisheries in the Central Amazon: a path toward tragedy?

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    Luiz Cláudio Pinto de Sá Alves

    2012-10-01

    Full Text Available Dolphin interactions with fishermen have increased significantly and pose potential risks to the boto, Inia geoffrensis (Blainville, 1817, and the tucuxi, Sotalia fluviatilis (Gervais & Deville, 1853. The main objective of the present paper was to describe the existing conflicts between river dolphins and fishermen in the municipality of Manacapuru region. Sixteen fishermen were interviewed in Manacapuru, state of Amazonas, Brazil who described a situation of ongoing conflict that may be unsustainable. Two merchants from Manacapuru made unconfirmed reports on a boto carcass trade. Data collection for this study occurred between April 20th and April 25th, 2009, but the first author had been conducting research on river dolphins and fisheries in Manacapuru and nearby cities since the beginning of 2008, in order to gain the trust of the fishermen interviewed. The hunting and deliberate killing of the species is probably more threatening to botos than their incidental capture in fishing gears in the Manacapuru region. This practice may result from the fact that dolphins are prone to damaging fishing equipment, and stealing (and possibly damaging fish from the nets. They are portrayed negatively in numerous myths and superstitions of traditional Amazonian folklore, making them extremely undesired or even hated, seen as pests, and used in the piracatinga, Calophysus macropterus (Lichtenstein, 1819 fishery as bait. For tucuxis, incidental capture still represents the major threat to their conservation in the region evaluated here.

  1. Antibacterial activity of Iranian medicinal plants against Streptococcus iniae isolated from rainbow trout (Oncorhynchus mykiss

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    Pirbalouti Ghasemi Abdollah

    2011-01-01

    Full Text Available Streptococcus iniae is among the major pathogens of a large number of fish species cultured in fresh and marine recirculating and net pen production systems. Ten Iranian medicinal plants were assessed for their antimicrobial activity against Streptococcus iniae isolates obtained from diseased Oncorhynchus mykiss (Salmonidae; Walbaum, 1972 collected from fish farms in Iran. The antibacterial activity of ethanol extracts of Punica granatum, Quercus branti, Glycyrrhiza glabra and essential oils of Heracleum lasiopetalum, Satureja bachtiarica, Thymus daenensis, Myrtus communis, Echinophora platyloba, Kelussia odoratissima and Stachys lavandulifolia against Steptococcus iniae was evaluated by disc diffusion and serial dilution assays. Most of the extracts and essential oils showed a relatively high antibacterial activity against Streptococcus iniae. Of the plants studied, the most active extracts were those obtained from the essential oils of Satureja bachtiarica, Echinophora platyloba, Thymus daenensis and the ethanol extract of Quercus branti. Some of the extracts were active against Streptococcus iniae. Two essential oils showed lower MIC values; Heracleum lasiopetalum (78 μg/ml and Satureja bachtiarica (39 μg/ml. The essential oil of Satureja bachtiarica could be an important source of antibacterial compounds against the Streptococcus iniae isolated from rainbow trout.

  2. Characterization of genetic coefficients of durum wheat (Triticum turgidumL. ssp. durum'Llareta-INIA' and 'Corcolén-INIA'

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    Marco Garrido

    2013-06-01

    Full Text Available The genetic coefficients, representative of a crop, are necessary to use growth models; in many cases the range of the species is known, but there are no specific values for varieties. Durum wheat (Triticum turgidumL. ssp. durum'Llareta-INIA' and 'Corcolén-INIA' were sown under irrigation in the experiment station Antumapu of the Universidad de Chile on 29 June 2007, 19 May 2009, and 15 June 2010 in order to determinate four genetic coefficients: developmental thermal time, phyllochron (PHLN, extinction coefficient (k, and radiation use efficiency (RUE, and to evaluate the performance of these coefficients. Thermal times to anthesis and from anthesis to physiological maturity were 652 and 541 °C d, respectively. Two PHLN values were found, 46.6 °C d leaf-1 from emergence to fifth leaf and 102.9 °C d leaf-1 from fifth to eighth leaf. In both cases neither variety nor sowing date was significant. The value of kdid not vary among sowing dates for 'Llareta-INIA' and was not significantly different from that of 'Corcolén-INIA' for normal planting date, due to which a single fit for the two varieties was adjusted, obtaining a value of 0.46. However, 'Corcolén-INIA' had a lower value of kfor later sowing dates. RUE did not vary between varieties or sowing dates; its estimated value was 2.83 g MJ-1. The evaluation of the coefficients obtained showed coherent results, thus they can be used in model simulations.

  3. Rapid Detection and Identification of Streptococcus Iniae Using a Monoclonal Antibody-Based Indirect Fluorescent Antibody Technique

    Science.gov (United States)

    Streptococcus iniae is among the major pathogens of a large number of fish species cultured in fresh and marine recirculating and net pen production systems . The traditional plate culture technique to detect and identify S. iniae is time consuming and may be problematic due to phenotypic variations...

  4. Controlled challenge experiment demonstrates substantial additive genetic variation in resistance of Nile tilapia (Oreochromis niloticus) to Streptococcus iniae

    Science.gov (United States)

    Streptococcus iniae is an etiologic agent of streptococcal disease in tilapia and is one of several Streptococcus spp. that negatively impact worldwide tilapia production. Methods for the prevention and control of S. iniae include vaccines, management strategies, and antibiotics. A complimentary pre...

  5. Anotaciones etnohistòricas sobre los indígenas botos: confluencia de datos históricos, antropológicos y de la tradición oral malecu

    OpenAIRE

    Constenla Umaña, Adolfo; Ibarra Rojas, Eugenia

    2014-01-01

    El siguiente trabajo comprende un ejercicio metodológico basado en la compilación de ocho historias sobre los botos, narradas por informantes guatusos y recogidas por Adolfo Constenla Umaña, aunque no tienen la traducción literal. A su solicitud, presento aquí las narraciones de las pláticas, y agrego un contexto histórico que contribuya a conocer la historia de los botos y los guatusos en el pasado. En esta tarea interdisciplinaria, es posible ver cómo la Lingüística, la Historia, y la Antro...

  6. Fatal septicemia caused by the zoonotic bacterium Streptococcus iniae during an outbreak in Caribbean reef fish.

    Science.gov (United States)

    Keirstead, N D; Brake, J W; Griffin, M J; Halliday-Simmonds, I; Thrall, M A; Soto, E

    2014-09-01

    An outbreak of Streptococcus iniae occurred in the early months of 2008 among wild reef fish in the waters of the Federation of St Kitts and Nevis, lasting almost 2 months. Moribund and dead fish were collected for gross, histological, bacteriological, and molecular analysis. Necropsy findings included diffuse fibrinous pericarditis, pale friable livers, and serosal petechiation. Cytological and histological analysis revealed granulocytic and granulomatous inflammation with abundant coccoid bacterial organisms forming long chains. Necrosis, inflammation, and vasculitis were most severe in the pericardium, meninges, liver, kidneys, and gills. Bacterial isolates revealed β-hemolytic, Gram-positive coccoid bacteria identified as S. iniae by amplification and 16S ribosomal RNA gene sequencing. Results from biochemical and antimicrobial susceptibility analysis, together with repetitive element palindromic polymerase chain reaction fingerprinting, suggest that a single strain was responsible for the outbreak. The inciting cause for this S. iniae-associated cluster of mortalities is unknown.

  7. Marine Animal SOUND Database

    Science.gov (United States)

    1991-08-01

    BG1A Susu .indii Blyth 1859 BG1B . Inia geoffrensis Blainville 1817 BG2A Lipotes vexillifer Miller 1918 BG3A £Po~ria blainvillei (Gervais) 1844 BG4A...river) -- Inia geoffrensis BG2A Yangtze river dolphin -- Lipotes vexillifer BG3A Franciscana -- Pontoporia blainvillei BG4A Seals, Sea otters, etc

  8. Spring forward with improved Nile tilapia Oreochromis niloticus resistant to Streptococcus iniae and Streptococcus agalactiae IB

    Science.gov (United States)

    Tilapia aquaculture worldwide is valued around US $ 7 billion. Tilapia are an important source of protein for domestic (top 5 most consumed seafoods) and global food security. Two gram postitive bacteria, Streptococcus iniae and S. agalactiae, are responsible for billion dollar losses annually. Gen...

  9. Experimental study to evaluate the pathogenicity of Streptococcus iniae in Guppy (Poecilia reticulata

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    Milad Adel

    2015-01-01

    Full Text Available Streptococcus iniae has emerged as an important fish pathogen over the last decade in farmed rainbow trout in Iran. The main objective of this study was to evaluate the pathogenicity of S. iniae in Poecilia reticulata. Atotal of 60 apparently healthy P. reticulata were obtained from ornamental fish pet store and injected intraperitoneally with 1.5×106 cfu of bacteria. For 14 days after challenge, the rate of mortality and clinical signs were recorded. The first clinical signs was observed in challenged fish 48 hrs after injection of S. iniae and first mortality was observed 72 hrs after injection. No significant differences in mortality and clinical signs between both sexes were observed. Streptococcus iniae was collected from internal organs of fishes challenged, and was confirmed using the conventional biochemical tests and PCR. It is concluded that, P. reticulata is susceptible to streptococcosis and can play an important role in transmission of the disease to other ornamental fish species and also cultured fish.

  10. Streptococcus iniae SF1: complete genome sequence, proteomic profile, and immunoprotective antigens.

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    Bao-cun Zhang

    Full Text Available Streptococcus iniae is a Gram-positive bacterium that is reckoned one of the most severe aquaculture pathogens. It has a broad host range among farmed marine and freshwater fish and can also cause zoonotic infection in humans. Here we report for the first time the complete genome sequence as well as the host factor-induced proteomic profile of a pathogenic S. iniae strain, SF1, a serotype I isolate from diseased fish. SF1 possesses a single chromosome of 2,149,844 base pairs, which contains 2,125 predicted protein coding sequences (CDS, 12 rRNA genes, and 45 tRNA genes. Among the protein-encoding CDS are genes involved in resource acquisition and utilization, signal sensing and transduction, carbohydrate metabolism, and defense against host immune response. Potential virulence genes include those encoding adhesins, autolysins, toxins, exoenzymes, and proteases. In addition, two putative prophages and a CRISPR-Cas system were found in the genome, the latter containing a CRISPR locus and four cas genes. Proteomic analysis detected 21 secreted proteins whose expressions were induced by host serum. Five of the serum-responsive proteins were subjected to immunoprotective analysis, which revealed that two of the proteins were highly protective against lethal S. iniae challenge when used as purified recombinant subunit vaccines. Taken together, these results provide an important molecular basis for future study of S. iniae in various aspects, in particular those related to pathogenesis and disease control.

  11. Evolution of the capsular operon of Streptococcus iniae in response to vaccination.

    Science.gov (United States)

    Millard, Candice M; Baiano, Justice C F; Chan, Candy; Yuen, Benedict; Aviles, Fabian; Landos, Matt; Chong, Roger S M; Benedict, Suresh; Barnes, Andrew C

    2012-12-01

    Streptococcus iniae causes severe septicemia and meningitis in farmed fish and is also occasionally zoonotic. Vaccination against S. iniae is problematic, with frequent breakdown of protection in vaccinated fish. The major protective antigens in S. iniae are the polysaccharides of the capsule, which are essential for virulence. Capsular biosynthesis is driven and regulated by a 21-kb operon comprising up to 20 genes. In a long-term study, we have sequenced the capsular operon of strains that have been used in autogenous vaccines across Australia and compared it with the capsular operon sequences of strains subsequently isolated from infected vaccinated fish. Intriguingly, strains isolated from vaccinated fish that subsequently become infected have coding mutations that are confined to a limited number of genes in the cps operon, with the remainder of the genes in the operon remaining stable. Mutations in strains in diseased vaccinated fish occur in key genes in the capsular operon that are associated with polysaccharide configuration (cpsG) and with regulation of biosynthesis (cpsD and cpsE). This, along with high ratios of nonsynonymous to synonymous mutations within the cps genes, suggests that immune response directed predominantly against capsular polysaccharide may be driving evolution in a very specific set of genes in the operon. From these data, it may be possible to design a simple polyvalent vaccine with a greater operational life span than the current monovalent killed bacterins.

  12. The INIA19 template and NeuroMaps atlas for primate brain image parcellation and spatial normalization

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    Torsten eRohlfing

    2012-12-01

    Full Text Available The INIA19 is a new, high-quality template for imaging-based studies of non-human primate brains created from high-resolution T1-weighted magnetic resonance (MR images of 19 rhesus macaque (Macaca mulatta animals. Combined with the comprehensive cortical and subcortical label map of the NeuroMaps atlas, the INIA19 is equally suitable for studies requiring both spatial normalization and atlas label propagation. Population-averaged template images are provided for both the brain and the whole head, to allow alignment of the atlas with both skull-stripped and unstripped data, and thus to facilitate its use for skull stripping of new images. This article describes the construction of the template using freely-available software tools, as well as the template itself, which is being made available to the scientific community (http://nitrc.org/projects/inia19/.

  13. Streptococcus iniae M-like protein contributes to virulence in fish and is a target for live attenuated vaccine development.

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    Jeffrey B Locke

    Full Text Available BACKGROUND: Streptococcus iniae is a significant pathogen in finfish aquaculture, though knowledge of virulence determinants is lacking. Through pyrosequencing of the S. iniae genome we have identified two gene homologues to classical surface-anchored streptococcal virulence factors: M-like protein (simA and C5a peptidase (scpI. METHODOLOGY/PRINCIPAL FINDINGS: S. iniae possesses a Mga-like locus containing simA and a divergently transcribed putative mga-like regulatory gene, mgx. In contrast to the Mga locus of group A Streptococcus (GAS, S. pyogenes, scpI is located distally in the chromosome. Comparative sequence analysis of the Mgx locus revealed only one significant variant, a strain with an insertion frameshift mutation in simA and a deletion mutation in a region downstream of mgx, generating an ORF which may encode a second putative mga-like gene, mgx2. Allelic exchange mutagenesis of simA and scpI was employed to investigate the potential role of these genes in S. iniae virulence. Our hybrid striped bass (HSB and zebrafish models of infection revealed that M-like protein contributes significantly to S. iniae pathogenesis whereas C5a peptidase-like protein does not. Further, in vitro cell-based analyses indicate that SiMA, like other M family proteins, contributes to cellular adherence and invasion and provides resistance to phagocytic killing. Attenuation in our virulence models was also observed in the S. iniae isolate possessing a natural simA mutation. Vaccination of HSB with the Delta simA mutant provided 100% protection against subsequent challenge with a lethal dose of wild-type (WT S. iniae after 1,400 degree days, and shows promise as a target for live attenuated vaccine development. CONCLUSIONS/SIGNIFICANCE: Analysis of M-like protein and C5a peptidase through allelic replacement revealed that M-like protein plays a significant role in S. iniae virulence, and the Mga-like locus, which may regulate expression of this gene, has an

  14. Development of a multiplex PCR assay to detect Edwardsiella tarda, Streptococcus parauberis, and Streptococcus iniae in olive flounder (Paralichthys olivaceus).

    Science.gov (United States)

    Park, Seong Bin; Kwon, Kyoung; Cha, In Seok; Jang, Ho Bin; Nho, Seong Won; Fagutao, Fernand F; Kim, Young Kyu; Yu, Jong Earn; Jung, Tae Sung

    2014-01-01

    A multiplex PCR protocol was established to simultaneously detect major bacterial pathogens in olive flounder (Paralichthys olivaceus) including Edwardsiella (E.) tarda, Streptococcus (S.) parauberis, and S. iniae. The PCR assay was able to detect 0.01 ng of E. tarda, 0.1 ng of S. parauberis, and 1 ng of S. iniae genomic DNA. Furthermore, this technique was found to have high specificity when tested with related bacterial species. This method represents a cheaper, faster, and reliable alternative for identifying major bacterial pathogens in olive flounder, the most important farmed fish in Korea.

  15. Immunological enhancement action of endotoxin-free tilapia heat shock protein 70 against Streptococcus iniae.

    Science.gov (United States)

    Chen, Ming; Wang, Rui; Li, Liping; Liang, Wanwen; Wang, Qiuhua; Huang, Ting; Li, Chao; Li, Jian; Gan, Xi; Lei, Aiying; Huang, Weiyi; Luo, Honglin

    2014-07-01

    The immunological effects of heat shock proteins (HSPs) had been found in humans and mice, but scarce data of endotoxin-free Hsp70 were reported in tilapia. In the current study, we reported that tHsp70 alone and antigen-tHsp70 compound increased the proliferations of lymphocytes and macrophages, significantly increased the NO release and phagocytotic ability of macrophages (ptilapia lymphocytes and macrophages post S. iniae exposure and its up-regulation effects on vaccine-induced protection. Our research highlights the immunological enhancement action of Hsp70 in teleost immunity.

  16. Recombinant thioredoxin 1 protein: the immune-adjuvant effect of Streptococcus iniae and its safety in rock bream, Oplegnathus fasciatus.

    Science.gov (United States)

    Kim, Ju-Won; Shim, Sang Hee; Lee, Jung-Ho; Jeong, Ji-Min; Park, Chan-Il

    2014-08-01

    Adjuvant is an immune enhancer commonly used during vaccination to enhance the host immune response. In the present study, we analysed the recombinant protein produced from rock bream thioredoxin 1 cDNA (rRbTRx1). To verify the immune-stimulatory effect of this recombinant protein, changes in the expression level of several genes were investigated using the cDNA microarray chips in rock bream peripheral blood leukocytes stimulated with rRbTRx1. Furthermore, the immune responses of rock bream to Streptococcus iniae FKC (formalin-killed cell) vaccination alone or in combination with recombinant proteins were analysed after an experimental challenge with living S. iniae. Microarray analysis showed that 237 unique genes were upregulated more than two-fold after rRbTRx1 stimulation. Serum agglutination titres were relatively low; however, the FKC vaccine still conferred protection against S. iniae. Moreover, the adverse effects showed no statistically significant difference between fish injected with a concentration and non-injected fish. After experimental challenge to the rock bream by injection with living bacteria (S. iniae), the relative percent survival in the vaccinated groups with FKC + rRbTRx1 was significantly higher than that of the vaccinated groups with FKC alone, which were 85.9% and 68.2%, respectively. This indicated that the recombinant protein as an adjuvant showed synergism with the injected vaccine in rock bream.

  17. Additive genetic variation in resistance of Nile tilapia (Oreochromis niloticus) to Streptococcus iniae and S. agalactiae capsular type Ib: is genetic resistance correlated?

    Science.gov (United States)

    Streptococcus (S.) iniae and S. agalactiae are both economically important Gram positive bacterial pathogens affecting the globally farmed tilapia (Oreochromis spp.). Historically control of these bacteria in tilapia culture has included biosecurity, therapeutants and vaccination strategies. Genet...

  18. Variation in the immunoglobulin levels in turbot (Scophthalmus maximus) after vaccination with Streptococcus iniae

    Institute of Scientific and Technical Information of China (English)

    ZHAN Wenbin; LIU Hongming; XING Jing; SHENG Xiuzhen; TANG Xiaoqian

    2009-01-01

    A pathogenic bacterium (S636), identified as Streptococcus iniae, was isolated from turbot (Scophthalmus maximus) in 2005. We immunized turbot with formalin-killed S. iniae four times (on days 1, 14, 21, and 28) by intraperitoneal inoculation. After each vaccination, we obtained serum samples and isolated the lymphocytes from the peripheral blood, spleen, pronephros, and mesonephros. We measured surface Ig-positive (sIg+) lymphocytes and serum antibody levels from these organs using flow cytometry and enzyme-linked immunosorbent assay (ELISA), respectively, using monoclonal antibodies against turbot immunoglobulin. We confirmed that the antibody reacted with both the surface and plasma Ig by confocal laser scanning microscopy and electron microscopy. The percentage of sIg+ in the lymphocytes increased following each successive vaccination. The mean percentage increased from 31.96% (control) to 37.49%, 38.36%, 42.9%, and 51.63% in the peripheral blood; from 27.09% to 36.63%, 36.81%, 39.28%, and 46.0% in the spleen; from 22.2% to 28.99%, 29.21%, 32.83%, and 41.58% in pronephros; and from 18.12% to 22.17%, 22.45%, 25.69%, and 31.68% in the mesonephros. The ELISA results were consistent with these results. Both the total and specific antibody levels increased with each vaccination. The mean OD value of the specific antibody assay increased from 0.094, to 0.269, 0.283, 0.333, and 0.421; for total antibody the mean OD value increased from 0.133, to 0.292, 0.323, 0.413, and 0.527.

  19. Valinia INIA, a New Early Storage Onion Variety for Central South Chile Valinia INIA, una Nueva Variedad de Cebolla de Guarda Precoz para la Zona Centro Sur de Chile

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    María Inés González A

    2012-03-01

    Full Text Available In Central South Chile, storage onion varieties (Allium cepa L. sold domestically usually begin the maturation process in March, which implies that a large proportion of bulbs do not mature adequately and do not resist prolonged storage. This situation led the Quilamapu vegetable program of the Instituto de Investigaciones Agropecuarias INIA to select an early maturing onion variety. This breeding method involved a recurrent selection with free plant crossing that began in 1994 on a population of 2000 plants of the 'Valenciana' commercial variety. Plants were first selected when they reached physiological maturity (tops down before 28 February, and then for shape, size, storage capacity, and single-centered bulbs. The result of the program was 'Valinia INIA', a type of Valenciana onion variety: early maturing, medium-sized bulb, spherical, yellow, and resistant to sprouting during storage. From the 2001-2002 to the 2008-2009 season, variety assays were carried out to compare 'Valinia INIA' with commercial cultivars, i.e., 'Sintética 14', 'Grano de Oro', and 'Valenciana Cobra'. 'Valinia INIA' precocity exceeded the other varieties in all seasons with a mean of 1.5 wk ahead of 'Sintética 14', which is the earliest commercial variety and more than 2 wk ahead of the other two. Its yield is similar to 'Valenciana Cobra' and higher than the other two varieties. Its storage capacity is similar to 'Grano de Oro' and 'Sintética 14', but greater than 'Valenciana Cobra'.En la zona centro sur de Chile es habitual que las variedades de cebolla (Allium cepa L. de guarda que se comercializan en el pais inicien el proceso de maduración preferentemente en marzo, lo que conlleva a que una gran proporción de bulbos no madure adecuadamente, no resistiendo el almacenaje por un tiempo prolongado. Esta situación llevó al programa de hortalizas del Instituto de Investigaciones Agropecuarias INIA Quilamapu a la selección de una variedad de cebolla de madurez

  20. Object carrying as socio-sexual display in an aquatic mammal.

    Science.gov (United States)

    Martin, A R; da Silva, V M F; Rothery, P

    2008-06-23

    Amazon river dolphins or botos (Inia geoffrensis Blainville) were observed carrying objects in 221 social groups over a 3-year study period. Sticks, branches and clumps of grass were taken from the water surface and often repeatedly thrashed or thrown. Lumps of hard clay were collected from the river bed and held in the mouth while the carrier rose slowly above the surface and submerged again. Carriers were predominantly adult males and less often subadult males. Adult females and young dolphins rarely carried objects. Groups of dolphins in which object carrying occurred were differentially large and comprised a greater proportion of adult males and adult females. Aggression, mostly between adult males, was significantly associated with object carrying. The behaviour occurred year-round, with peaks in March and July. A plausible explanation of the results is that object carrying by adult males is aimed at females and is stimulated by the number of females in the group, while aggression is targeted at adult males and is stimulated by object carrying in the group. We infer that object carrying in this sexually dimorphic species is socio-sexual display. It is either of ancient origin or has evolved independently in several geographically isolated populations.

  1. Minor contribution of mutations at iniA codon 501 and embC-embA intergenic region in ethambutol-resistant clinical Mycobacterium tuberculosis isolates in Kuwait

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    Ahmad Suhail

    2009-01-01

    Full Text Available Abstract Background Ethambutol (EMB is a first-line drug for the treatment of tuberculosis (TB. Resistance to EMB in Mycobacterium tuberculosis isolates is mediated by mutations in several genes involved in arabinan synthesis notably three emb (arabinosyl transferase and iniA (isoniazid-inducible genes. Most epidemiologically unrelated EMB-resistant M. tuberculosis strains contain mutations at embB codons 306, 406 and 497, embC-embA intergenic region (IGR and iniA codon 501 (iniA501. Objective To develop a more comprehensive molecular screen for EMB-resistance detectioamong epidemiologically unrelated EMB-resistant M. tuberculosis strains previously analyzed for embB codon 306, 406 and 497 mutations by including analysis of mutations at iniA501 and in embC-embA IGR. Methods Fifty consecutive and phenotypically documented EMB-resistant and 25 pansusceptible M. tuberculosis strains isolated from 75 different TB patients over a four-year period in Kuwait were analyzed. Mutations at iniA501 were detected by PCR amplification followed by restriction fragment length polymorphism (RFLP patterns generated with Hpy 99 I. Direct DNA sequencing was used to confirm RFLP results and for detecting mutations in embC-embA IGR. Results Nearly same number of EMB-resistant M. tuberculosis strains were resistant to EMB alone and EMB together with additional resistance to rifampicin and isoniazid (9 of 50, 18% and 11 of 50, 22%, respectively. All the 25 pansusceptible strains contained wild-type sequences at iniA501 and in embC-embA IGR. The analysis of 50 EMB-resistant M. tuberculosis isolates showed that only one strain contained a mutated iniA501 while no mutation was detected in embC-embA IGR in any of the isolate. Conclusion Analysis of iniA501 and embC-embA IGR in epidemiologically unrelated EMB-resistant M. tuberculosis isolates in Kuwait indicate that mutations at these locations occur very infrequently and their inclusion for the development of a

  2. Growth performance and resistance to Streptococcus iniae of juvenile Nile tilapia (Oreochromis niloticus) fed diets supplemented with GroBiotic - A and Brewtech Dried Brewers Yeast

    Science.gov (United States)

    This study was conducted to evaluate the effect of dietary levels of Brewtech® dried brewers yeast (BY) and GroBiotic®-A (GB) on growth performance, proximate body composition, immune response and resistance of juvenile Nile tilapia to Streptococcus iniae challenge. A practical basal (control) diet ...

  3. Effects of dietary levels of vitamin A on growth, hematology, immune response and resistance of Nile tilapia (Oreochromis niloticus) to Streptococcus iniae

    Science.gov (United States)

    This study was conducted to evaluate the effect of supplemental levels of vitamin A (0, 2,500, 5,000, 10,000, and 20,000 IU/kg diet) on the growth performance, hematology, immune response and resistance of Nile tilapia, Oreochromis niloticus to Streptococcus iniae challenge. Each diet was fed to Nil...

  4. Complete Genome Sequence of Streptococcus iniae UEL-Si1, Isolated in Diseased Nile Tilapia (Oreochromis niloticus) from Northern Paraná, Southern Brazil

    Science.gov (United States)

    Gonçalves, Kátia B.; Scarpassa, Josiane A.; Pretto-Giordano, Lucienne G.

    2017-01-01

    ABSTRACT The Streptococcus iniae UEL-Si1 strain was isolated from diseased Nile tilapia within the Paranapanema River Basin, Northern Paraná, Brazil. This is an emerging infectious disease agent of fish from Brazil, and sequencing of the complete genome is fundamental to understanding aspects relative to pathogenesis, infection, epidemiology, and immunity. PMID:28082497

  5. Phenotypic and genotypic heterogeneity among Streptococcus iniae isolates recovered from cultured and wild fish in North America, Central America and the Caribbean Islands

    Science.gov (United States)

    Streptococcus iniae, the etiological agent of streptococcosis in fish, is an important pathogen of cultured and wild fish worldwide. During the last decade outbreaks of streptococcosis have occurred in a wide range of cultured and wild fish in the Americas and Caribbean islands. To gain a better und...

  6. Fitness cost, gyrB mutation, and absence of phosphotransferase system fructose specific IIABC component in novobiocin-resistant Streptococcus iniae vaccine strain ISNO

    Science.gov (United States)

    To understand the fitness cost of novobiocin-resistance in an attenuated Streptococcus iniae vaccine strain ISNO compared to its virulent parent strain ISET0901, cell proliferation rate of the two strains were compared to each other. Our results revealed that the cell proliferation rates of ISNO wer...

  7. Effectiveness of Aquaflor (50% florfenicol) administered in feed to control mortality associated with Streptococcus iniae in tilapia at a commercial tilapia production facility

    Science.gov (United States)

    Gaikowski, Mark P.; Schleis, Susan M.; Leis, Eric; Lasee, Becky A.; Endris, Richard G.

    2014-01-01

    The efficacy of Aquaflor (florfenicol; FFC) to control mortality caused by Streptococcus iniae in tilapia was evaluated under field conditions. The trial was initiated following presumptive diagnosis of S. iniae infection in a mixed group of fingerling (mean, 4.5 g) Nile Tilapia Oreochromis niloticus and a hybrid of Nile Tilapia×Blue Tilapia O. aureus. Diagnoses included mortality in source tank; examination of clinical signs and presence or absence of gram-positive cocci in brain, and collection of samples for microbiological review and disease confirmation of 60 moribund fish. Following presumptive diagnosis, tilapia (83/tank) were randomly transferred to each of 20 test tanks receiving the same water as the source tank (test tank water was not reused). Tilapia were offered either nonmedicated control feed or FFC-medicated feed (FFC at 15 mg/kg body weight/d; 10 tanks per regimen) for 10 consecutive days followed by a 14-d observation period during which only the nonmedicated control feed was offered. Streptococcus iniae was presumptively identified during pretreatment necropsy and confirmed by polymerase chain reaction assay; S. iniae was confirmed in samples taken during the dosing period but was not detected during the postdosing period. The FFC disk diffusion zone of inhibition ranged from 29 to 32 mm, while the minimum inhibitory concentration of FFC ranged from 2 to 4 μg/mL for the S. iniae isolates collected. Survival of tilapia assigned to the FFC-dose group was significantly greater at 14 d posttreatment than that of the nonmedicated controls. The odds of tilapia assigned to the FFC-dose group surviving to the end of the postdosing period were 1.34 times the odds of survival of tilapia assigned to the nonmedicated control group. There were no clinically apparent adverse effects associated with the administration of FFC-medicated feed in this study.

  8. Multiplex PCR for simultaneous detection of Streptococcus agalactiae, Streptococcus iniae and Lactococcus garvieae: a case of S. agalactiae infection in cultured Nile tilapia (Oreochromis niloticus) and red tilapia (Oreochromis niloticus x Oreochromis mossambicus)

    OpenAIRE

    Chutima Tantikitti; Naraid Suanyuk; Akkarawit Itsaro

    2012-01-01

    A multiplex PCR (m-PCR) technique was developed for simultaneous detection of the causative agents responsible forstreptococcosis of cultured fish in Thailand i.e., Streptococcus agalactiae, Streptococcus iniae, and Lactococcus garvieae.The study on the sensitivity of the technique indicated that the minimum detected DNA concentration was 9.76, 39.06, and19.53 pg for S. agalactiae, S. iniae and L. garvieae, respectively. Detection of streptococcosis in healthy and diseased Niletilapia (Oreoch...

  9. Efficacy of QCDCR formulated CpG ODN 2007 in Nile tilapia against Streptococcus iniae and identification of upregulated genes.

    Science.gov (United States)

    Pridgeon, Julia W; Klesius, Phillip H; Mu, Xingjiang; Yancey, Robert J; Kievit, Michele S; Dominowski, Paul J

    2012-01-15

    The potential of using a QCDCR (quilA:cholesterol:dimethyl dioctadecyl ammonium bromide:carbopol:R1005 glycolipid) formulated CpG oligodeoxynucleotide (ODN), ODN 2007, to confer protection in Nile tilapia against Streptococcus iniae infection was evaluated in this study. At two days post treatment, QCDCR formulated ODN 2007 elicited significant (Psubtractive cDNA library. Quantitative PCR revealed that 44 ESTs were significantly (P10-fold) and 15 moderately (<10-fold) upregulated ESTs. Of all ESTs, putative peroxisomal sarcosine oxidase was upregulated the highest. The 69 ESTs only included six genes that had putative functions related to immunity, of which only two (putative glutaredoxin-1 and carboxypeptidase N catalytic chain) were confirmed to be significantly upregulated. Our results suggest that the protection elicited by ODN 2007 is mainly through innate immune responses directly or indirectly related to immunity.

  10. Descrição do comportamento de superfície do boto cinza, Sotalia guianensis, na Praia de Pipa - RN Description of the surface behaviour of marine tucuxi, Sotalia guianensis, at Pipa Beach - RN

    Directory of Open Access Journals (Sweden)

    Lídio França do Nascimento

    2008-01-01

    Full Text Available O estudo do comportamento animal é uma área de pesquisa consolidada, que cresceu e se diversificou em vários países e que deu origem a disciplinas e abordagens de investigação, como a Etologia, a Ecologia Comportamental, a Neuroecologia, a Psicologia e a Psicologia evolucionista. No estudo do comportamento animal, o etograma é a base para estudos comportamentais de espécies pouco conhecidas, como também para comparar comportamentos de populações distintas de uma mesma espécie. Tradicionalmente, um etograma descreve de forma detalhada eventos comportamentais realizados por indivíduos de uma espécie. O presente estudo apresenta um etograma dos eventos comportamentais realizados na superfície por uma espécie de golfinho costeiro, boto cinza, na praia de Pipa - RN.Research in animal behavior is a consolidated area whose development and diversification in several countries have originated some subjects and investigation approaches, such as Ethology, Behavior Ecology, Neuroecology, Psychology and Evolutionist Psychology. In the study of animal behavior, the ethogram is the foundation for studies on behavior patterns of distinct populations of the same species. An ethogram traditionally describes behavioral events performed by individuals of the same species in detail. This study presents an ethogram of behavioral events performed on surface by a costal dolphin known as the gray dolphin (Marine Tucuxi Dolphin, at Pipa Beach, RN.

  11. Construction of a Streptococcus iniae sortase A mutant and evaluation of its potential as an attenuated modified live vaccine in Nile tilapia (Oreochromis niloticus).

    Science.gov (United States)

    Wang, J; Zou, L L; Li, A X

    2014-10-01

    Streptococcus iniae is a major Gram-positive aquatic pathogen, which causes invasive diseases in cultured fish worldwide. The identification of potential virulence determinants of streptococcal infections will help to understand and control this disease, but only a few have been confirmed in S. iniae. Sortase A (srtA) is the key enzyme that anchors pre-mature cell wall-attached proteins to peptidoglycan and it can affect the correct positioning of surface proteins, as well as the course of Gram-positive bacterial infection, thereby making it a potential target in the study of virulence factors and disease control. In this study, the 759 bp srtA gene was cloned from pathogenic S. iniae TBY-1 strain and the mutant strain TBY-1ΔsrtA was constructed via allelic exchange mutagenesis. We found that srtA shares high similarities with sortase A from other Streptococcus spp. Direct survival rate assay and challenge experiments were performed, which showed that the mutant strain TBY-1ΔsrtA had a lower survival capacity in healthy tilapia blood and it was less virulent than the wild type strain in tilapia, thereby indicating that the deletion of sortase A affects the virulence and infectious capacity of S. iniae. The mutant strain TBY-1ΔsrtA was used as a live vaccine, which was administered via intraperitoneal injection, and it provided the relative percent survival value of 95.5% in Nile tilapia, thereby demonstrating its high potential as an effective attenuated live vaccine candidate.

  12. Comparación de las características físicas de las fibras de la llama ch´aku (Lama glama) y la alpaca Huacaya (Lama pacos) del centro experimental Quimsachata del INIA – Puno

    OpenAIRE

    2009-01-01

    Universidad Nacional Agraria La Molina. Escuela de Posgrado. Maestría en Producción Animal Los objetivos fueron evaluar los efectos de especie y sexo sobre las características físicas de las fibras de llama Ch´aku y alpaca Huacaya de un año de edad, del Centro Experimental Quimsachata del INIA – Puno y determinar las correlaciones de las principales características. Las muestras de fibra se obtuvieron del C.E. Quimsachata del INIA, ubicada en distrito de Santa Lucia (Puno-Perú); y fueron a...

  13. PADRÃO COMPORTAMENTAL E PERCEPÇÃO DOS BARQUEIROS SOBRE O BOTO TUCUXI, Sotalia fluviatilis (ODONTOCETI: DELPHINIDAE EM ÁREA DO ENTORNO DA ILHA DE SANTANA, AMAPÁ, BRASIL

    Directory of Open Access Journals (Sweden)

    Luana Caroline Souza Barroso

    2015-12-01

    Full Text Available A espécie Sotalia fluviatilis é pouco conhecida na região amazônica, principalmente no estado do Amapá, onde estudos são bastante escassos. O presente estudo tem por objetivo contribuir para o aumento do conhecimento sobre o padrão comportamental e percepção dos barqueiros sobre S. fluviatilis no porto do açaí. Este trabalho foi realizado em um ponto fixo localizado no Porto do Açaí. As observações foram feitas mensalmente no período de julho de 2013 a dezembro de 2013 em período matutino, junto às entrevistas com os barqueiros presentes no porto com a aplicação de um questionário de pesquisa com TCLE. Os padrões comportamentais mais frequentes observados pelo tucuxi foram deslocamento lento, deslocamento rápido e forrageio. Com relação a percepção dos barqueiros quando perguntados a maior parte dos barqueiros afirmou ver o boto tucuxi com frequência no porto, em grupos de dois a três indivíduos, realizando atividade de alimentação/forrageio. A presença maior da espécie no período de maré alta foi afirmada pelos entrevistados, corroborando com os dados de observação in loco. Segundo os entrevistados o tucuxi quando avistado localiza-se as margens do rio e que a presença da espécie é atribuída à alimentação descartada pelos vendedores de peixe do local e que hoje há menos animais em relação à 5 anos. Nesse sentido, espera-se o presente estudo contribua para informações sobre áreas de ocorrência de S. fluviatilis e futuramente subsidiar medidas de conservação para a espécie, tendo em vista a iminência da construção de um porto de escoamento de soja nas proximidades da área. Palavras-chave: Conhecimento popular, Etologia, região norte, cetáceos. DOI: http://dx.doi.org/10.18561/2179-5746/biotaamazonia.v5n4p119-124

  14. Host-derived probiotics Enterococcus casseliflavus improves resistance against Streptococcus iniae infection in rainbow trout (Oncorhynchus mykiss) via immunomodulation.

    Science.gov (United States)

    Safari, Reza; Adel, Milad; Lazado, Carlo C; Caipang, Christopher Marlowe A; Dadar, Maryam

    2016-05-01

    The present study evaluated the benefits of dietary administration of host-derived candidate probiotics Enterococcus casseliflavus in juvenile rainbow trout Oncorhynchus mykiss. Experimental diets were prepared by incorporating the microorganisms in the basal feed at 3 inclusion levels (i.e. 10(7) CFU g(-1) of feed [T1], 10(8) CFU g(-1) of feed [T2], 10(9) CFU g(-1) of feed [T3]). The probiotic feeds were administered for 8 weeks, with a group fed with the basal diet serving as control. The effects on growth performance, gut health, innate immunity and disease resistance were evaluated. Results showed that growth performance parameters were significantly improved in T2 and T3 groups. Activities of digestive enzymes such as trypsin and lipase were significantly higher in these two groups as well. Gut micro-ecology was influenced by probiotic feeding as shown by the significant increase in intestinal lactic acid bacteria and total viable aerobic counts in T2 and T3. Humoral immunity was impacted by dietary probiotics as total serum protein and albumin were significantly elevated in T3. The levels of serum IgM significantly increased in all probiotic fed groups at week 8; with the T3 group registering the highest increment. Respiratory burst activity of blood leukocytes were significantly improved in T2 and T3. Hematological profiling further revealed that neutrophil counts significantly increased in all probiotic fed groups. Challenge test showed that probiotic feeding significantly improved host resistance to Streptococcus iniae infection, specifically in T2 and T3 where a considerable modulation of immune responses was observed. Taken together, this study demonstrated E. casseliflavus as a potential probiotics for rainbow trout with the capability of improving growth performance and enhancing disease resistance by immunomodulation.

  15. O Desenvolvimento do Turismo Náutico e a sua Ligação com a Observação do Boto-Cinza (sotalia guianensis na Região de Cananéia, Litoral Sul do Estado de São Paulo

    Directory of Open Access Journals (Sweden)

    Gislaine de Fátima Filla

    2010-07-01

    Full Text Available Resumo O boto-cinza é hoje um grande atrativo e gerador de renda para a região estuarina de Cananéia. O crescimento da infra-estrutura turística desta região parece estar intimamente relacionado com o crescimento do interesse em observar estes animais na natureza. Assim, um levantamento das atuais estruturas de alojamento e capacidade náutica da região foi feito nos meses de verão de 2007, através de visitas aos estabelecimentos e entrevistas com pessoas atuantes no turismo náutico. Ficou evidente que as estruturas de alojamento e o uso das embarcações nas três ilhas do estuário variam de acordo com as diferentes categorias de turistas que freqüentam esta região e com o objetivo de suas viagens. Ainda, foi possível notar que a organização em associações e parcerias garantiu um crescimento maior da atividade de observação do boto-cinza, a qual deve ser acompanhada com cuidado para que seja desenvolvida de forma otimizada, conservando os animais e seu habitat natural. Palavras-chaves: boto-cinza; sotalia guianensis; turismo náutico; Cananéia Abstract The estuarine dolphin (sotalia guianensis is a big attraction and money maker for the Cananéia estuary region. The increased tourist infrastructure of the region seems to be directly related to the growing interest to observe these animals in loco. Therefore, a survey was done to asses the present boarding and nautical capacity of the region during the summer months of 2007, by means of visits to the establishments and interviews with the people working with nautical tourism. It was evident that the boarding structures and the use of ships on the three islands of the estuary vary according to the different type of tourists that visit the area and their reasons for the trip. Even so, it was possible to note that organized associations and partnerships guaranteed a larger growth and activity of estuarine dolphin observation, which must be carefully followed to ensure proper

  16. Location of an acoustic window in dolphins.

    Science.gov (United States)

    Popov, V V; Supin, A Y

    1990-01-15

    Auditory brainstem responses (ABR) to sound clicks from sources in different positions were recorded in dolphins Inia geoffrensis. The position of the acoustic window was determined by measurement of acoustic delays. The acoustic window was found to lie close to the auditory meatus and the bulla rather than on the lower jaw.

  17. Dietary Aloe vera improves plasma lipid profile, antioxidant, and hepatoprotective enzyme activities in GIFT-tilapia (Oreochromis niloticus) after Streptococcus iniae challenge.

    Science.gov (United States)

    Gabriel, Ndakalimwe Naftal; Qiang, Jun; Ma, Xin Yu; He, Jie; Xu, Pao; Liu, Kai

    2015-10-01

    The current study investigated the effects of dietary Aloe vera on plasma lipid profile status, antioxidant, and hepatoprotective enzyme activities of GIFT-tilapia juveniles under Streptococcus iniae challenge. Five dietary groups were designed including a control and 100 % Aloe powder incorporated into a tilapia feed at 0.5, 1, 2, and 4 %/kg feed, which were administered for 8 weeks. Fish fed dietary Aloe at 4 %/kg feed significantly reduced in total cholesterol, while triacylglycerol reduced (P Aloe/kg feed compared to unsupplemented ones. High-density lipoprotein was significantly elevated in fish fed 0.5 and 1 % Aloe/kg feed compared to unsupplemented ones, and no significant changes (P > 0.05) were noted in low-density lipoprotein among test groups. Furthermore, high activities of superoxide dismutase, catalase, and glutathione peroxide in liver tissues were observed in Aloe-supplemented fish compared to unsupplemented ones, before and after S. iniae challenge (7.7 × 10(6) CFU cells/mL). Variations were also noted in malondialdehyde activity throughout the trial, but no significant difference (P > 0.05) was observed between groups. Meanwhile, Aloe-supplemented fish reduced serum aspartate and alanine aminotransferase (AST and ALT) activities before and after challenge. Based on the second-order polynomial regression analysis, dietary Aloe inclusion levels less than or equal to 1.88, 1.86, and 2.79 %/kg feed were determined to be suitable in improving plasma lipid profile status, antioxidant, and hepatoprotective enzyme activities in GIFT-tilapia in this study, respectively. Thus, A. vera extracts may be recommended as a tilapia feed supplement to enhance fish antioxidant and hepatoprotective capacities, especially during disease outbreaks.

  18. PUBLIC-PRIVATE ALLIANCES FOR INNOVATION: THE CASE OF THE CONSORCIOS REGIONALES DE INNOVACIÓN (REGIONAL CONSORTIUM FOR INNOVATION LED BY THE NATIONAL INSTITUTE OF LIVESTOCK INVESTIGATION OF URUGUAY(INIA

    Directory of Open Access Journals (Sweden)

    Rodrigo Saldías

    2012-03-01

    Full Text Available The public-private partnerships represent a strategy for improving the efficiency in the generation of innovations in a global context, where the increasing of competitiveness is crucial for companies. Although the public and business environment present different interests, it is possible to establish a common area for this type of alliance, oriented to the generation of knowledge in a further conscious environment of business and productive realities. As an example of this type of alliance, the National Institute of Livestock Investigation of Uruguay (INIA is in the process of implementing the Regional Consortium for Innovation (CRI in the production chain of milk in Uruguay, in order to facilitate the development of research and development activities oriented to innovation. Thus, this paper, through an exploratory and documentary survey, describes the example of the INIA and, based on the literature about public-private partnerships, discusses its importance in the development of Uruguay.

  19. Dietary Aloe vera supplementation on growth performance, some haemato-biochemical parameters and disease resistance against Streptococcus iniae in tilapia (GIFT).

    Science.gov (United States)

    Gabriel, Ndakalimwe Naftal; Qiang, Jun; He, Jie; Ma, Xin Yu; Kpundeh, Mathew D; Xu, Pao

    2015-06-01

    This study investigated effects of dietary Aloe vera on growth performance, some haemato-biochemical parameters and disease resistance against Streptococcus iniae in tilapia (GIFT). Five groups were designed including a basal diet (control) and 100% A. vera powder incorporated in fish feed at 0.5% 1%, 2%, and 4%/kg feed, which were administered for 8 weeks. Fish fed 0.5%, 1%, and 2% A. vera supplemented diet significantly improved (p vera diet at 1% and 2%/kg feed. Feed efficiency ratio, feed conversion ratio, and hepatosomatic index were significantly enhanced in 4% A. vera supplemented fish over unsupplemented ones (p vera supplemented fish showed a significant increase (p vera supplemented fish showed a decrease (p vera diet at 2% and 4% A. vera/kg feed than those fed unsupplemented diet. Unchallenged fish fed 0.5%, 1%, and 2% A. vera showed significantly higher values (p vera supplemented diet. There was a significant increase (p vera unsupplemented fish and those supplemented with A. vera diet at 1%/kg feed increased significantly (p vera supplemented diet maintained higher values at all experimental stages among groups. There was a significant correlation (p Aloe had no significant effect (p > 0.05) on the survival of the fish when compared to the control; no mortality was recorded in challenge trial. Overall, our results indicated that dietary aloe supplementation could improve growth, feed utilization, and haemato-biochemical parameters of cultured tilapia.

  20. Seasonal and daily patterns of group size, cohesion and activity of the estuarine dolphin, Sotalia guianensis (P.J. van Bénéden (Cetacea, Delphinidae, in southern Brazil Padrões sazonais e diários do tamanho de grupo, coesão e atividade do boto-cinza, Sotalia guianensis (P.J. van Bénéden (Cetacea, Delphinidae, no sul do Brasil

    Directory of Open Access Journals (Sweden)

    Fábio G. Daura-Jorge

    2005-12-01

    Full Text Available The resident population of estuarine dolphin, Sotalia guianensis (P.J. van Bénéden, 1864, in Norte Bay, Santa Catarina, southern Brazil, was studied from 2000 to 2003. Systematic one-day cruises were undertaken aboard a 5 m sail boat equipped with an outboard motor. Applying the focal-group method, information such as geographical position, predominant behavioural patterns, group size, cohesion index and the presence of calves, were registered at every five-minute interval, totalling 198 hours of direct observation of the dolphins. The estuarine dolphin used preferential areas for each behavioural pattern. The mean group size was approximately 29 individuals, and the presence of calves occurred throughout the entire year, but with a significant increase in the warm seasons. The frequency of behavioural patterns and group size varied according to season and time of day, and were strongly associated. The frequency of moving behaviours increased in the cold seasons and along the day. The seasonal variation in the moving behaviours suggest the requirement of a larger area in the cold seasons, probably related to seasonal fluctuations in the abundance of dominant prey items. Higher group sizes were observed while dolphins were foraging. The cohesion index also varied according to the behaviour. Our results showed that group organization and behavioural patterns of this estuarine dolphin population are probably linked to daily and seasonal environment cycles, and also possibly to the condition of being at the furthest southern limit of distribution of the species.A população residente de boto-cinza, Sotalia guianensis (P.J. van Bénéden, 1864, da Baía Norte, Santa Catarina, sul do Brasil, foi estudada de 2000 a 2003. Foram realizados cruzeiros sistemáticos de um dia, a bordo de um veleiro de 5 m equipado com motor de popa. Utilizando o método de grupo-focal, informações como posição geográfica, padrão comportamental predominante, tamanho

  1. Blind river dolphin: first side-swimming cetacean.

    Science.gov (United States)

    Herald, E S; Brownell, R L; Frye, F L; Morris, E J; Evans, W E; Scott, A B

    1969-12-12

    The blind river dolphin (Platanista gangetica), first written about by Pliny the Elder in A.D. 72, was found (10 November 1968) to be the first known side-swimming cetacean. The rudimentary eye lacks the lens, but anatomical evidence suggests that the eye may serve as a light sensor. The underwater sound emissions of this species, although similar to those of the Amazon River dolphin (Inia geoffrensis), appear to be produced constantly.

  2. Effects of Nitrogen on Productivity, Grain Quality, and Optimal Nitrogen Rates in Winter Wheat cv. Kumpa-INIA in Andisols of Southern Chile Efecto del Nitrógeno en Productividad, Calidad del Grano y Dosis Óptimas de Nitrógeno en Trigo Invernal cv. Kumpa-INIA en Andisoles del Sur de Chile

    Directory of Open Access Journals (Sweden)

    Ricardo Campillo

    2010-03-01

    Full Text Available Nitrogen is one of the main inputs of the winter wheat crop (Triticum aestivum L. in southern Chile. Nitrogen efficient management is basic to optimizing its utilization while decreasing pollution risks and operational costs. Crop response and N use efficiency (NUE, defined as the ratio of yield to mineral N supply, regardless of source are important for evaluating N requirements of winter wheat, and reaching maximum and economic yields. The objective of this study was to determine the effect of N rate on grain yield, calculate the N rate that maximizes yield, and estimate the optimal grain yield rate and quality of high-yielding winter wheat cv. Kumpa-INIA. Five annual N rates were evaluated in a randomized complete block design during two successive winter wheat cropping seasons on a Vilcún series soil of the Pachic Melanudands family (Andisol in La Araucania Region, Chile, and subjected to intensive annual crop rotation. Significant effects (P ≤ 0.01 of N rate on grain yield and quality were found. The optimal physical N rate (OPR in both seasons ranged from 290 to 339 kg ha-1, whereas optimal economic N rate (OER ranged from 248 to 274 kg ha-1, with yields between 10.2 and 10.1 t ha-1. Nitrogen use efficiency associated to OER was high in both seasons (36.9 and 41.2 kg grain kg-1 N and fluctuated in similar ranges. Nitrogen rate increased hectoliter weight and grain protein, but decreased NUE.El N es uno de los principales insumos del cultivo de trigo (Triticum aestivum L. en Chile. Su manejo eficiente optimiza las dosis, disminuye los riesgos de contaminación y los costos de producción. La respuesta del cultivo y eficiencia de uso de N (NUE, definida como la razón de rendimiento y suministro de N mineral, independientemente de la fuente son importantes para evaluar los requerimientos de N y alcanzar rendimientos máximos y económicos. El objetivo de este estudio fue determinar el efecto del N en la producción y las dosis que

  3. 杂交鳢海豚链球菌病病原分离鉴定及药敏试验%Isolation and identification of pathogen Streptococcus iniae in diseased hybrid snakehead (Channa argus♂× Channamaculata♀)

    Institute of Scientific and Technical Information of China (English)

    陈言峰; 周爱国; 邹青; 陈冠锋; 陈建酬; 张继平; 张辉华

    2015-01-01

    【目的】对广东省中山市某养殖场的患病濒死杂交鳢进行病原分离鉴定及药敏试验,旨在确定病因,为今后生产中有效防控该病提供参考。【方法】从濒死杂交鳢体内分离致病菌,通过其生理生化特征与16S rRNA基因序列分析进行菌株鉴定,并采用纸片扩散法进行药敏试验。【结果】从患病濒死的杂交鳢体内分离获得两株优势菌(ZS2013711-1和ZS2013711-2),根据其生理生化鉴定结果与16S rRNA基因序列分析结果,可鉴定为海豚链球菌(Streptococcusiniae)。两株分离菌株对杂交鳢的半致死剂量(LD50)分别为2.7×105和6.7×105 CFU/g。药敏试验结果显示,该致病菌对头孢噻肟、头孢拉定、头孢唑啉、林可霉素等抗生素高度敏感。【结论】杂交鳢是海豚链球菌的一种新发现宿主,生产中可选用头孢噻肟、头孢拉定、头孢唑啉、林可霉素等药物进行防治。%Objective]The pathogens from diseased hybrid snakehead (Channa argus♂×Channa maculata♀) in an aquafarm in Zhongshan City of Guangdong Province were isolated and identified and antibiotic sensitivity test was per-formed in order to determine the causes and provide references for preventing and controlling this disease effectively.[Method]Pathogenic bacteria were isolated from moribund hybrid snakehead , and they were identified through physio-logical and biochemical characteristics test and 16S rRNA sequence analysis. Furthermore , antibiotic sensitivity test was performed through Kirby-Bauer disk diffusion method. [Result]Two dominant bacterias (ZS2013711-1, ZS2013711-2) were isolated from moribund hybrid snakehead , and they were identified to be Streptococcus iniae according to their physiological and biochemical characteristics and 16S rRNA sequence analysis. The half lethal dose values for the iso-lates to healthy hybrid snakehead were 2.7 ×105 and 6.7 ×105 CFU/g, respectively. The results of

  4. Preliminary study on immune effect of Streptococcus iniae inactivated vaccine in Oreochromis niloticus%海豚链球菌灭活疫苗对罗非鱼免疫效果的初步研究

    Institute of Scientific and Technical Information of China (English)

    张俊; 黄木珍; 李安兴

    2011-01-01

    Formalin killed vaccines with/without white oil adjuvant were prepared with TBY-1 strain of Streptococcus iniae isolated from freshwater fish. The efficacy of the vaccines were determined in Oreochromis niloticus by bath immersion and intraperitoneal (IP) injection successively. The relative percent survival (RPS) of each immune group was statisticsed. Moreover, the length and weight of the tilapias were measured and analyzed by SPSS. The results showed that the RPS of the fish immuned with bath immersion and then immunized intraperitoneally once with none-adjuvant vaccine and white oil adjuvant were 50. 50% and 71. 10% respectively, and the antibody agglutination titer were 1:4. 8 and 1: 128 respectively. The RPS of fish immuned with bath immersion and then immunized intraperitoneally twice with none-adjuvant vaccine and white oil adjuvant were 82. 50% and 58. 80% respectively, and the antibody agglutination titer were 1∶6.4 and 1∶27.4 re spectively.%将海豚链球菌(Streptococcus iniae)淡水分离株TBY-1菌株灭活后制备成无佐剂和添加白油佐剂的疫苗,利用浸泡和腹腔注射复合免疫方式对罗非鱼(Oreochromis niloticus)进行免疫,比较其相对保护率及血清中抗体凝集效价,以评价佐剂的存在与否及免疫次数对海豚链球菌灭活疫苗的免疫效果的影响,同时还对实验前后罗非鱼的体长体重进行测量和统计,并用SPSS软件进行分析.结果显示,用无佐剂疫苗进行首次浸泡免疫后,再分别用无佐剂疫苗及白油佐剂疫苗注射免疫1次的相对保护率分别为50.50%和71.10%,血清中抗体凝集效价分别为1∶4.8和1∶128;而注射免疫2次的相对保护率分别为82.50%和58.80%,其血清凝集效价分别为1∶6.4和1∶27.4.

  5. Cloning,identification and molecular characteristics analysis of simA gene of a Streptococcus iniae strain DGX07 from channel catfish%斑点叉尾(鮰)源海豚链球菌DGX07株simA基因克隆、鉴定及分子特性分析

    Institute of Scientific and Technical Information of China (English)

    汪开毓; 王均; 肖丹; 陈德芳; 黄锦炉; 黄凌远; 付希; 王浩丞

    2012-01-01

    以Primer p14(5’-GATCAAGTCC-3’)为引物对分离自患病斑点叉尾(鮰)海豚链球菌强毒株DGX07进行RAPD分析.同时参照GenBank中海豚链球菌simA基因序列设计特异性引物,以DGX07基因组DNA为模板,扩增出约1 500 bp的simA基因,并将其克隆到pMD19-T载体上,之后对重组质粒进行PCR和双酶切(BamH Ⅰ+Xho Ⅰ)鉴定,鉴定正确后送测序公司测序.RAPD分析结果显示,DGX07和标准菌株均能扩增出750 bp大小的条带.通过生物信息学软件对测序结果分析显示,simA基因全长1 566bp,由521个氨基酸组成,与海豚链球菌simA和simB亲缘性达100%,存在1个由41个氨基酸组成的信号肽,具有Bap31和Gram_pos_anchor两个超家族的保守结构域;具有与蛋白翻译后修饰功能相关的磷酸化位点22个和N-糖基化位点2个,编码多肽链中亲水区大于疏水区,是一种膜外蛋白,并具有多个抗原优势位点区域.密码子偏爱性分析表明,斑点叉尾(鮰)源海豚链球菌simA基因密码子使用频率差异较大,密码子偏爱性与酵母较为接近.获得GenBank登录号为JF330100.%The RAPD analysis of Streptococcus iniae virulent strain DGX07 isolated from channel catfish (Ictalurus punctatus)v/as conducted by PCR with Primer pl4(5'-GATCAAGTCC-3'). The simA gene of DGX07 was amplified by PCR with specific primers and cloned into pMD19-T vector. The positive plasmid was selected and identified by PCR and digestion of double restriction enzyme (BamH I + Xho I ). Molecular characterization analysis of simA gene was performed by bioinformatics tools. The results showed that S. Iniae simA amino acid sequence was highly conservative and with 100% homology to the simA and simB of S. Iniae isolated. The polypeptide contained a signal peptide comprising 41 amino acids and Bap31 and Gram _ pos _ anchor functional domains. The polypeptide had some important sites related to post-translational modification, including 22 phosphorylation sites and 2 N

  6. Interaural intensity and latency difference in the dolphin's auditory system.

    Science.gov (United States)

    Popov, V V; Supin AYa

    1991-12-09

    Binaural hearing mechanisms were measured in dolphins (Inia geoffrensis) by recording the auditory nerve evoked response from the body surface. The azimuthal position of a sound source at 10-15 degrees from the longitudinal axis elicited interaural intensity disparity up to 20 dB and interaural latency difference as large as 250 microseconds. The latter was many times greater than the acoustical interaural time delay. This latency difference seems to be caused by the intensity disparity. The latency difference seems to be an effective way of coding of intensity disparity.

  7. Functional Morphology and Symmetry in the Odontocete Ear Complex.

    Science.gov (United States)

    Ary, William; Cranford, Ted W; Berta, Annalisa; Krysl, Petr

    2016-01-01

    Odontocete ear complexes or tympanoperiotic complexes (TPCs) were compared for asymmetry. Left and right TPCs were collected from one long-beaked common dolphin (Delphinus capensis) and one Amazon River dolphin (Inia geoffrensis). Asymmetry was assessed by volumetric comparisons of left and right TPCs and by visual comparison of superimposed models of the right TPC to a reflected mirror image of the left TPC. Kolmogorov-Smirnov tests were performed to compare the resonant frequencies of the TPCs as calculated by vibrational analysis. All analyses found slight differences between TPCs from the same specimen in contrast to the directional asymmetry in the nasal region of odontocete skulls.

  8. 海豚链球菌感染对不同品系罗非鱼血液生化指标和肝脏HSP70 mRNA表达的影响%Studies on blood biochemical indices and expression of hepatic HSP70 mRNA of different tilapia strains artificially challenged with Streptococcus iniae

    Institute of Scientific and Technical Information of China (English)

    强俊; 杨弘; 王辉; 徐跑; 柒壮林; 何杰

    2012-01-01

    In order to establish the superiority, four different tilapia strains (GIFT tilapia, New GIFT tilapia, Egypt Nile tilapia and Red tilapia) were compared in the study. At day 100 tilapias in the treatment group were intraperitoneally injected with Streptococcus iniae suspension (2.95×108/mL) to evaluate disease resistance of four different tilapia strains against S. iniae in terms of the serum biochemical parameters and hepatic HSP70 mRNA expression before and after infection. Another set of twenty fish taken from each tank was challenged with S. iniae using the same method as above, and the cumulative mortality of 4 strains was recorded at different times post infection. The results showed that GIFT tilapia and New GIFT tilapia were more sensitive to S. iniae at 96 h after infection, cumulative mortalities were 36.67% and 38.33% respectively. Egypt Nile tilapia was less sensitive to pathogen and no death occurred during the experiment. The levels of serum cortisol (COR) and glucose (GLU) and expression levels of HSP70 mRNA in GIFT tilapia, New GIFT tilapia and Red tilapia strains were highly significant(P<0.05), and the activities of glutamic-pyruvic transaminase (ALT), glutamic-oxaloacetic transaminase (AST)and lysozyme (LSZ) also increased, while serum alkaline phosphatase (AKP), triglyceride and cholesterol levels were significantly lower than those prior to infection (P<0.05). By utilizing the energy from resolved hepatin and lipid, the synthesis of hepatic HSP70 mRNA and levels of LSZ and globulin in Egypt Nile tilapia were improved, and thus the non-specific immunity strengthened. It is suggested that in the course of tilapia breeding, effective combination of the disease resistance with growth be conducted so as to guarantee sustainable development of the tilapia industry.%以吉富罗非鱼、新吉富罗非鱼、埃及尼罗罗非鱼和红罗非鱼为研究对象,饲养100 d后,进行海豚链球菌(2.95×108 CFU/mL)感染试验,分析攻毒前后各品

  9. Estimación de la repetitividad y selección genética de árboles de cacao aromático con material genético de EE-INIA-San Martin y de la UC de Lebuaf, en Perú

    Directory of Open Access Journals (Sweden)

    Carlos Oliva

    2014-01-01

    Full Text Available El cultivo de cacao, es una de las actividades más importantes en la Amazonía Peruana. Su base genética estásustentada en la introducción de clones mejorados, por lo que el potencial genético local que están en losbancos de germoplasmano está siendo bien utilizados debido al limitado estudio en mejoramiento genético.Este trabajo tuvo por objetivo estimar la repetibilidad para la selección genética de árboles con altorendimiento de granos de cacao aromático. El análisis de selección genética fue ejecutado con el softwareSELEGEN REML/BLUP, en una data de 3 años de evaluación de rendimiento de granos kg/árbol, pesopromedio de granos (g y peso promedio de mazorcas (kg en el banco de Germoplasma de INIA-San Martin yUnidad de Conservación de Lebuaf SAC. La repetibilidad individual para rendimiento de granos/árbol y pesopromedio de granos fue moderada con (0,52 y (0,30 respectivamente, lo que no ocurrió con el pesopromedio de la mazorca que fue baja con (0,24. La variable con mayor estabilidad fue el rendimiento degranos/árbol, quien arrojo en 3 cosechas 0,77, propiciando una exactitud selectiva de 0,88. Genotipossuperiores pueden ser seleccionados con precisión con 4 a 5 cosechas. La selección y clonación de los 20mejores genotipos o árboles selectos deberán propiciar una ganancia genética de 112%, elevando laproductividad media anual de granos por árbol de 1,24 kg/árbol a 1,54kg/árbol.

  10. Parasitism affects vaccine efficacy against Streptococcus iniae in Nile tilapia

    Science.gov (United States)

    Tilapia culture worldwide is estimated to be US$ 5 billion and is important to domestic and global food security. Parasites and bacteria co-occur in both extensive and intensive production of tilapia. The effect of parasitism on vaccine performance in fish is little studied. The objective of this ...

  11. Lipids of freshwater Dolphin Sotalia fluviatilis: comparison of odontocete bioacoustic lipids and habitat.

    Science.gov (United States)

    Ackman, R G; Eaton, C A; Kinneman, J; Litchfield, C

    1975-01-01

    The melon and jaw lipids of the freshwater dolphin Sotalia fluviatilis are composed mainly of isovaleroyl wax esters and diisovaleroyl triglycerides. The blubber fat contains only a trace of wax ester and is mostly tri-(long chain) and monoisovaleroyl triglycerides. Detailed gas liquid chromatographic analyses of the intact wax esters and triglycerides and of the derived fatty acids and fatty alcohols indicate common compositional patterns in the wax esters and triglycerides of the respective head lipids. Both odd and even long chain (C12-C16) isostructures are prominent in the melon and jaw lipids, but only higher odd chain length iso-acids are major components in the blubber. Sotalia fluviatilis (family Delphinidae) and Inia geoffrensis (family Platanistidae) share the same freshwater habitat in the upper Amazon River, and both utilize echolocation to navigate and to find food. Comparison of their respective bioacoustical lipid compositions show distinctive types of head fats, Sotalia being rich in iso-5:0 and Inia lacking iso-5:0. This indicates that iso-valeric acid per se has no obligatory role in dolphin echolocation.

  12. Contribuições do comportamento acústico do boto-da-tainha (Tursiops truncatus) para o entendimento da interação entre botos e pescadores em Laguna, Santa Catarina

    OpenAIRE

    Romeu, Bianca

    2015-01-01

    Dissertação (mestrado) - Universidade Federal de Santa Catarina, Centro de Ciências Biológicas, Programa de Pós-Graduação em Ecologia, Florianópolis, 2015. Após décadas de estudos em cativeiro, nos últimos anos os avanços no conhecimento sobre a bioacústica dos odontocetos têm contado com estudos com animais de vida livre. Tais estudos permitem a investigação da influência de aspectos comportamentais e sociais no repertório acústico destes animais, possibilitando a compreensão sobre como o...

  13. The evaluation of the risk for floodings on the territory of Botoșani municipal town

    Directory of Open Access Journals (Sweden)

    Delia Nicoleta VIERU

    2010-04-01

    Full Text Available The county of Botosani is not placed in an area of high risk for flooding, because most of the lands within the build-up area are situated on the ledge. Because of the increase ofthe population, some socio-economical objectives have been located in the high risk area on the basis of the ledge; it’s necessary that the real insurance and that provided by therules could be established, thereby, the following measures need to be established: the achievement of field works against floods, the gad of the buildings exposed to the majorrisk. The real insurance of the buildings located on the four effluents (located at the base of the ledge, varies between 5 – 10 per cent, in comparison to 1 per cent as it is foreseen bythe rules. The dams built up on these valeys, made up of local materials and generally used for fishy needs, have large uploaders calculated under 1 per cent error, which might lead to dangerous waves of flood, in case of river burst. The possible damages have both direct (material and human losses and indirect results (traffic jams, coming up to normal conditions, and the price of the operative measures for preventing human victims The estimation of the damages to certain flood is inconclusive, the most important damage being the one afferent to the main class of the objective, without mentioning the ecological damages, as in case of extreme humidity, epidemic dangers, quick reproduction of mosquitos etc.

  14. Local ecological knowledge and the interaction of porpoises with fishing on the coast of the state of Para, Brazil

    Directory of Open Access Journals (Sweden)

    Tiago Pereira Brito

    2012-11-01

    Full Text Available Fishing has a great importance to the socioeconomic development of the Amazonian riparian communities, which have a local knowledge on the environment, its fauna and flora. This knowledge is of paramount importance for the management and conservation of fish stocks and species that interact with the fishing activity, such as the populations of gray porpoises (Sotalia sp. and pink porpoises (Inia geoffrensis. Therefore, to register local ecological knowledge and the interaction of porpoises with fishing on the coast of the state of Para, Brazil, 82 semi-structured interviews were carried out in 3 different colonies of fishermen: colony Z-1 (Soure, colony Z-7 (Maracana, and colony Z-23 (Colares. The species Sotalia sp. had a strong social structure, it can be observed in the region both during the dry and rainy seasons, while the species I. geoffrensis didn’t present a social structure, it’s observed in the region especially during the rainy season. The pink porpoise showed a larger interaction with fishing than the gray porpoise, however, this interaction was particularly detrimental to the activity, while the gray porpoise showed both a beneficial and a harmful interaction with fishing. The incidental capture of porpoises primarily occurred through nets and trawls, harming their populations. Captured animals were mostly released, however, there were records of consumption for food, use as bait, and selling of the animal’s eyes. This information will constitute the basis for further studies on the biology, ecology, and estimate of the mortality of porpoises on the Amazon coast. Management measures should be adopted, along with the communities studied, for the conservation of these animals in the region.

  15. Ascaridoid nematodes of South American mammals, with a definition of a new genus.

    Science.gov (United States)

    Sprent, J F

    1982-09-01

    Ascaridoid nematodes occurring in South American mammals are divided into categories based on their possible origin. The affinities are discussed of five species so far known only from the Neotropical Region. Toxocara alienata (Rudolphi 1819) is reported from Nasua rufa socialis, Procyon cancrivorus, and Tayassus torquatus. The specimens from T. torquatus are described and found most closely to resemble Toxocara mackerrasae from south-east Asian and Austrialian rodents. Anisakis insignis from Inia geoffrensis is transferred back to Peritrachelius Diesing, 1851, on account of the structure of the lips and spicules. P. insignis is shown to exhibit remarkable convergence of lip structure with Lagochilascaris turgida from Didelphis marsupialis. Galeiceps longispiculum (Freitas & Lent, 1941) from Pteronura brasiliensis is confirmed as a species distinct from G. cucullus (Linstow, 1899) and G. spinicollis (Baylis, 1923), but G. simiae (Mosgovoy, 1951) is considered to be a synonym of G. spinicollis. An error in the host record of G. spinicollis is corrected from Cercopithecus leucampyx kandti to Lutra maculicollis kivuana. Ascaris dasypodina Baylis, 1922 from armadillos, including Cabassous unicinctus and Tolypeutes matacos, is redescribed and placed in a new genus Bairdascaris. The question is raised as to whether some species in Lagochilascaris, Galeiceps, and Toxocara may have crossed directly by sea from Africa to South America, rather than entering via North America.

  16. Direction-dependent spectral sensitivity and interaural spectral difference in a dolphin: evoked potential study.

    Science.gov (United States)

    Supin AYa; Popov, V V

    1993-06-01

    Sensitivity and interaural intensity difference (IID) dependence on sound frequency and direction was measured in an Amazon river dolphin Inia geoffrensis by recording the auditory nerve evoked response from the body surface. The maximal sensitivity in the horizontal plane was found when the sound direction was 5 degrees to 10 degrees ipsilateral to the recorded ear; the direction dependence of sensitivity was more pronounced at higher frequencies than at lower ones. The IID reached its peak at small azimuthal angles (7.5 degrees to 15 degrees) and higher sound frequencies (100 kHz), or at large azimuthal angles (30 degrees to 45 degrees) and lower sound frequencies (20 to 30 kHz). Each sound direction featured its specific pattern of spectral sensitivity and of interaural spectral difference. The interaural spectral difference fluctuated within a range of more than 20 dB depending on sound direction. The data indicate that interaural intensity as well as spectral difference may be cues for binaural localization of sound direction by dolphins.

  17. Complete primary structure of the major component myoglobin of California gray whale (Eschrichtius gibbosus).

    Science.gov (United States)

    Bogardt, R A; Dwulet, F E; Lehman, L D; Jones, B N; Gurd, F R

    1976-06-15

    The complete primary structure of the major component myoglobin from the California gray whale, Eschrichtius gibbosus, was determined by specific cleavage of the protein to obtain large peptides for degradation by the automatic sequenator. Cleavage at the two methionine residues of the apomyoglobin with cyanogen bromide and at the three arginine residues of the methyl acetimidated protein with trypsin resulted in three and four easily separable peptides, respectively, which when sequenced accounted for 85% of the primary structure. The remainder of the covalent structure was obtained by further digestion of the central cyanogen bromide peptide with trypsin and S. aureus strain V8 protease. This protein differs from that of the sperm whale, Physeter catodon, at 12 positions, from that of the common porpoise, Phocoena phocoena, and the Black Sea dolphin, Delphinus delphis, at 14 positions, and from that of the Amazon River dolphin, Inia geoffrensis, at 7 positions. All substitutions observed in this sequence fit easily into the tertiary structure of sperm whale myoglobin.

  18. The complete amino acid sequence of the major component myoglobin from the arctic minke whale, Balaenoptera acutorostrata.

    Science.gov (United States)

    Lehman, L D; Dwulet, F E; Bogardt, R A; Jones, B N; Gurd, F R

    1977-02-22

    The complete primary structure of the major component myoglobin from the Arctic minke whale, Balaenoptera acutorostrata, was determined by specific cleavage of the protein to obtain large peptides which are readily degraded by the automatic sequencer. Over 80% of the amino acid sequence was established from the three peptides resulting from the cleavage of the apomyoglobin at the two methionine residues with cyanogen bromide along with the four peptides resulting from the cleavage of the methylacetimidated apomyoglobin at the three arginine residues with trypsin. The further digestion of the central cyanogen bromide peptide with trypsin and S. aureus strain V8 protease enabled the determining of the remainder of the covalent structure. This myoglobin differs from that of the dwarf sperm whale, Kogia simus, at 16 positions, and the common dolphin, Delphinus delphis, at 14 positions, from that of the common porpoise, Phocaena phocaena, and the bottlenosed dolphin, Tursiops truncatus at 13 positions, from that of the Amazon River dolphin, Inia geoffrensis, at 10 positions, and from that of California gray whale, Eschrichtius gibbosus, at 3 positions- All of the substitutions observed in this sequence fit easily into the three-dimensional structure of the sperm whale myoglobin.

  19. Riqueza de mamíferos de grande e médio porte do Parque Nacional da Serra do Divisor (Acre, Brasil The richness of the large and medium-sized mammals of the Serra do Divisor National Park (Acre, Brazil

    Directory of Open Access Journals (Sweden)

    Armando Muniz Calouro

    1999-01-01

    Full Text Available The objective of the present study was to characterize the richness (number of species of large and medium-sized mammals, and the antropic threats in the Parque Nacional da Serra do Divisor (PNSD. In 31 days data were collected through direct observations or evidences (bones, hairs, vocalizations and tracks along pre-existing trails distributed in different types of vegetation. Wild mammals captured by local people were also considered. They were found 44 species of terrestrial mammals (with the exception of small mammals and bats and two species of cetaceans, representing 73% of the total predicted, according to the literature and information of local dwellers were registered in PNSD. They exist in the area two species classified by IUCN (1996 as "Endangered" [Cacajao calvus rubicundus (I. Geoffroy, 1806 and Priodontes maximus (Kerr, 1792] and five as "Vulnerable" [Ateles chamek (Humboldt, 1812, Callimico goeldii (Thomas, 1904, Lagothrix lagotricha poeppigii (Humboldt, 1812, Myrmecophaga tridactyla Linnaeus, 1758 and Inia geoffrensis (Blainville, 1817]. Given that subsistence and commercial hunting are common in the PNSD, mammals more affected by hunting [Ateles chamek (Humboldt, 1812, Lagothrix lagotricha (Humboldt, 1812, Tayassu pecari (Link, 1795] were observed only in the more remote areas such as Serra do Divisor, Rio Moa. In comparison with others areas, the results indicate that PNSD has high richness of the mammals, with special attention to the 14 primates species registered.

  20. Antibacterial compounds from Rutaceae with activities against Flavobacterium columnare and Streptococcus iniae

    Science.gov (United States)

    Bioassay-guided fractionation of the ethyl acetate extract of Murraya koenegii (Rutaceae) leaves yielded isomahanine (1) and mahanine (2) with antibacterial activity towards bacteria species that cause columnaris disease and streptococcosis, common diseases in pond-raised channel catfish (Ictalurus ...

  1. Phylogeny of all major groups of cetaceans based on DNA sequences from three mitochondrial genes.

    Science.gov (United States)

    Milinkovitch, M C; Meyer, A; Powell, J R

    1994-11-01

    Traditionally, living cetaceans (order Cetacea) are classified into two highly distinct suborders: the echolocating toothed whales, Odontoceti, and the filter-feeding baleen whales, Mysticeti. A molecular phylogeny based on 1,352 base pairs of two mitochondrial ribosomal gene segments and the mitochondrial cytochrome b gene for all major groups of cetaceans contradicts this long-accepted taxonomic subdivision. One group of toothed whales, the sperm whales, is more closely related to the morphologically highly divergent baleen whales than to other odontocetes. This finding suggests that the suborder Odontoceti constitutes an unnatural grouping and challenges the conventional scenario of a long, independent evolutionary history of odontocetes and mysticetes. The superfamily Delphinoidea (dolphins, porpoises, and white whales) appears to be monophyletic; the Amazon River dolphin, Inia geoffrensis, is its sister species. This river dolphin is genetically more divergent from the morphologically similar marine dolphins than the sperm whales are from the morphologically dissimilar baleen whales. The phylogenetic relationships among the three families of Delphinoidea remain uncertain, and we suggest that the two cladogenetic events that generated these three clades occurred within a very short period of time. Among the baleen whales, the bowhead is basal, and the gray whale is the sister species to the rorquals (family Balaenopteridae). The phylogenetic position of beaked whales (Ziphioidea) remains weakly supported by molecular data. Based on molecular clock assumptions, the mitochondrial-DNA data suggest a more recent origin of baleen whales (approximately 25 mya) than has been previously assumed (> 40 mya). This revised phylogeny has important implications for the rate and mode of evolution of morphological and physiological innovations in cetaceans.

  2. Host-derived probiotics Enterococcus casseliflavus improves resistance against Streptococcus iniae infection in rainbow trout (Oncorhynchus mykiss) via immunomodulation

    DEFF Research Database (Denmark)

    Safari, Reza; Adel, Milad; Lazado, Carlo Cabacang;

    2016-01-01

    CFU g-1 of feed [T1], 108 CFU g-1 of feed [T2], 109 CFU g-1 of feed [T3]). The probiotic feeds were administered for 8 weeks, with a group fed with the basal diet serving as control. The effects on growth performance, gut health, innate immunity and disease resistance were evaluated.Results showed...... that growth performance parameters were significantly improved in T2 and T3 groups. Activities of digestive enzymes such as trypsin and lipase were significantly higher in these two groups as well. Gut micro-ecology was influenced by probiotic feeding as shown by the significant increase in intestinal lactic......The present study evaluated the benefits of dietary administration of host-derived candidate probiotics Enterococcus casseliflavus in juvenile rainbow trout Oncorhynchus mykiss. Experimental diets were prepared by incorporating the microorganisms in the basal feed at 3 inclusion levels (i.e. 107...

  3. Growth Performance and Resistance to Streptococcus iniae of Nile Tilapia, Oreochromis niloticus Fed Various Dietary Levels of Thiamin

    Science.gov (United States)

    Thiamin or vitamin B1 functions in all cells as the coenzyme thiamin pyrophosphate that involves in the oxidative decarboxylation of an alpha-keto acids and the transketolase reactions in the pentose phosphate pathway. Thiamin has been demonstrated to be essential in diets of fish, and quantitative ...

  4. Considerations on FEM modeling in analyzing buckling and plastic collapse of a detection control; Boto panel no zakutsu sosei hokai kaiseki ni okeru FEM model ka ni kansuru kosatsu

    Energy Technology Data Exchange (ETDEWEB)

    Yao, T.; Fujikubo, M.; Yanagihara, D.; Irisawa, M. [Hiroshima University, Hiroshima (Japan). Faculty of Engineering

    1997-10-01

    Buckling and plastic collapse of upper decks and bottom outer plates of a hull results directly in longitudinal bending collapse of the hull. Therefore, discussions were given on analysis for pressure destruction strength of a detection control panel which assumes an upper deck and a bottom outer plate. Pressure destruction behavior of the panting panel is a complex phenomenon accompanying non-linearity and geometrical non-linearity of the materials. Its whole phenomenon may be analyzed by using the finite element method (FEM) as a principle, but the analysis is not efficient. Therefore, considerations were given in relation to modeling when using the FEM. The considerations were given on a panel attached with flat steel panting members with respect to the modeling scope which considers the buckling mode according to the aspect ratio of the panel partitioned by the deflection control members. If the local buckling mode of the panel is an even number wave mode in the longitudinal direction, a triple span model is required. A modeling scope for a case of being subjected to water pressure and in-plane compression was considered on a panel attached with angle-type steel members having non-symmetric cross section. In this case, a triple bay model is more preferable to reproduce the behavior under water pressure loading. 1 ref., 6 figs.

  5. Description of the surface behaviour of marine tucuxi, Sotalia guianensis, at Pipa Beach - RN / Descrição do comportamento de superfície do boto cinza, Sotalia guianensis, na Praia de Pipa - RN

    Directory of Open Access Journals (Sweden)

    Lídio França do Nascimento

    2008-01-01

    Full Text Available Research in animal behavior is a consolidated area whose development and diversification in several countries have originated some subjects and investigation approaches, such as Ethology, Behavior Ecology, Neuroecology, Psychology and Evolutionist Psychology. In the study of animal behavior, the ethogram is the foundation for studies on behavior patterns of distinct populations of the same species. An ethogram traditionally describes behavioral events performed by individuals of the same species in detail. This study presents an ethogram of behavioral events performed on surface by a costal dolphin known as the gray dolphin (Marine Tucuxi Dolphin, at Pipa Beach, RN.

  6. Estimation of repeatability and genetic selection of aromatic cacao trees with genetic material from EE-INIA-San Martin and UC Lebuaf in Peru

    OpenAIRE

    Carlos Oliva; José Benito; Ronald Acuña; Ana Bocanegra; Jhordan Baltazar

    2014-01-01

    Cocoa farming is one of the most important activities in the Peruvian Amazon. Its genetic basis is supported by the introduction of improved clones, so that the local genetic potential are in germplasm bank is not well used due to limited breeding study. This study aimed to estimate the repeatability for the genetic selection of high-yield trees with aromatic cocoa beans. Genetic selection analysis was executed with the SOFTWARE SELEGEN REML / BLUP in a 3-year data for assessing grain yield k...

  7. Morfologia do sincrânio do boto-cinza, Sotalia guianensis (P.J. van Bénéden (Cetacea, Delphinidae Syncranial morphology of the estuarine dolphin Sotalia guianensis (P.J. van Bénéden (Cetacea, Delphinidae

    Directory of Open Access Journals (Sweden)

    Paulo C. Simões-Lopes

    2006-09-01

    Full Text Available Apresenta-se uma descrição da morfologia comparada do sincrânio de Sotalia guianensis (P.J. van Bénéden, 1864, incluindo suas variações ontogenéticas e intra-específicas. As descrições foram baseadas em 51 crânios completos ou parciais depositados na coleção do laboratório de Mamíferos Aquáticos, do Departamento de Ecologia e Zoologia, CCB, Universidade Federal de Santa Catarina. O sincrânio é bastante homogêneo e manifesta sua assimetria na morfologia dos ossos, passagens nasais e forames. O rostro é proporcionalmente estreito em relação aos outros Delphinidae do mesmo porte. A região temporal é circular em indivíduos jovens, mas se torna oval nos adultos onde se forma uma crista temporal evidente. O forame elíptico do periótico está presente tanto em indivíduos jovens quanto adultos. Os nasais são bipartidos e podem ocorrer ossos suturais entre os frontais. O processo hamular dos pterigóides é bastante inflado e fenestrado. A sínfise mandibular alcança de 19 de 19-23% do comprimento do dentário. Cada pré-maxilar e maxilar possui de 30-35 dentes e cada dentário de 30-38 dentes.Morphological and comparative description of the syncranium of Sotalia guianensis (P.J. van Bénéden, 1864, including ontogenic aspects and intraspecific variations are presented. The descriptions were based on the examination of 51 complete or partial skulls, kept at the Marine Mammals Lab. Collection from the Departamento de Ecologia e Zoologia, CCB, at the Federal University of Santa Catarina. The sincranium is very homogeneous and manifests its asymmetry in the morphology of the bones, nasal passages and foramina. The rostrum is proportionally narrow in relation to other Delphinidae of the same size. The temporal region is circular in young specimens, but it becomes oval in the adults were it forms a well marked temporal crest. The elliptical foramen of the periotic bone is present both in young and adult individuals. The nasals are bipartite and one may find sutural bones between the frontals. The hamular process of the pterygoids is inflated and somewhat fenestrated. The mandibular symphysis covers from 19-23% of the dentary length. Each premaxilla and maxilla has 30-35 teeth and each dentary exhibits 30-38 teeth.

  8. OPPORTUNITIES AND RISKS OF GLOBALIZATION IN RURAL AREAS. CASE STUDY: BOTOSANI COUNTY

    Directory of Open Access Journals (Sweden)

    Maria-Simona Cuciureanu

    2014-04-01

    Full Text Available The global economy plays an important role in the development of a region or a county/district as it promotes the exploitation of resources and space in a logical and rational manner. The trend of global economic uniformity allows opportunities and risks to Botoșani County since globalization involves economic development and rising living standards, but loss of cultural values, traditions and customs. The area of study currently confronts with socio-economic and demographic changes that may be addressed by globalization, but at the same time spatial development according to global standards will cause the loss of Botoșani County’s authenticity.

  9. Growth, body fatty acid composition, immune response and resistance to Streptococcus iniae of hybrid tilapia, Oreochromis niloticus X O. aureus, fed diets containing various levels of linoleic and linolenic acids

    Science.gov (United States)

    The effects of dietary linoleic (LA) and linolenic acids (LN) on growth and immunity of all-male hybrid tilapia, Oreochromis niloticus × O. aureus, were evaluated for 10 weeks. Fish fed 0.12% LA + 0% LN had the lowest weight gain (WG) but was not significantly different from diets containing 0.5% LA...

  10. Molecular epidemiology of zoonotic streptococcosis/lactococcosis in rainbow trout (Oncorhynchus mykiss) aquaculture in Iran

    DEFF Research Database (Denmark)

    Karsidani, S. Haghighi; Soltani, M.; Nikbakhat-Brojeni, G.

    2010-01-01

    Chinese, and some Taiwanian strains. Also, analysis of S. iniae LctO gene sequence showed that this isolate clustered within the S. iniae group. The sequence analysis of L. garvieae strains also showed that they have maximum similarity to all Japanese and Chinese strains, but one strain has lower sequence...

  11. Comportamento do boto-cinza, Sotalia guianensis (Van Bénédén (Cetacea, Delphinidae na presença de barcos de turismo na Praia de Pipa, Rio Grande do Norte, Brasil Behavior of estuarine dolphin, Sotalia guianensis (Van Bénédén (Cetacea, Delphinidae in the presence of tourist boats in Pipa Beach, Rio Grande do Norte, Brazil

    Directory of Open Access Journals (Sweden)

    Érico Santos-Jr

    2006-09-01

    Full Text Available A crescente indústria do turismo tem se estendido ao ambiente aquático, deste modo tornando os mamíferos aquáticos suscetíveis a interações com humanos. O turismo de observação de golfinhos na praia de Pipa litoral sul do Rio Grande do Norte, tem se desenvolvido aumentando o número de barcos de observação de golfinhos operando nesta área. No presente estudo alterações comportamentais do Sotalia guianensis (Van Bénédén, 1864 causado pela presença destes barcos foi verificada. Registros comportamentais de S. guianensis foram compilados de um ponto fixo do alto de uma falésia próxima à área de estudo. As observações foram realizadas quando não havia barcos, na presença de barcos e após a saída dos barcos. Os resultados mostraram que o tipo de aproximação praticado pelos barcos de observação de golfinhos tinha maior influência no comportamento dos animais, especialmente em grupos com filhotes. Os impactos de curto prazo foram mais discretos, no entanto, novos métodos devem ser aplicados no sentido de verificar impactos de longo prazo no comportamento dos golfinhos.The ever-increasing tourist industry has extended to the aquatic environments, thus making the aquatic mammals susceptible to interactions with humans. Dolphin watching tourism has developed to a great extent in the Pipa Beach, Rio Grande do Norte, consequently the number of dolphin-watching boats operating in this area has increased. In the present study the behavioral alterations of Sotalia guianensis (Van Bénédén, 1864 caused by the presence of these boats were verified. Behavioral registers of S. guianensis were compiled from a fixed point on the cliffs close to the study area. The observations were effected when there were no boats, in the presence of boats and after the departure of boats. The results show that type of approach by the dolphin watching boats had a major influence on the behavior of the dolphins, especially so in the groups with calves. The short term impacts were rather discrete, however, new methods should be applied in order to verify the long term impacts on the dolphins.

  12. Novel method of generating non-human transgenic animals, and transgenic animals thus obtained

    OpenAIRE

    Ventura Oliveira, Pedro Nuno; GUTIÉRREZ-ADÁN, Alfonso; Montoliu, Lluís

    2005-01-01

    Fecha de presentación internacional: 04.04.2005. - Titulares: Consejo Superior de Investigaciones Científicas (CSIC). - Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA)

  13. A STUDY ON THE GROWING OF ROMANIAN BLACK SPOTTED COWS IN SOME PRIVATE EXPLOITATIONS FROM COUNTY BOTOŞANI

    Directory of Open Access Journals (Sweden)

    RODICA DĂNĂILĂ

    2013-12-01

    Full Text Available The Romanian black spotted cows – a breed specialized in the milk production –represents the main supplier of milk in the country.The study was made to highlight the productive level and the power of hereditarytransmission of the main features thus offering data useful for the elaboration andapplication of the melioration programme for this breed in county Botoşani.

  14. Structural isomerism leading to variable proton conductivity in indium(III) isophthalic acid based frameworks.

    Science.gov (United States)

    Panda, Tamas; Kundu, Tanay; Banerjee, Rahul

    2013-07-14

    Proton conductivity has been studied thoroughly in two isomeric In(III)-isophthalate based MOFs. In-IA-2D-1 is capable of showing proton conductivity (3.4 × 10(-3) S cm(-1)) under humidified conditions (98% RH), whereas In-IA-2D-2 can conduct protons (2.6 × 10(-5) S cm(-1)) under humidified as well as anhydrous conditions.

  15. ANALYSIS OF THE PUBLIC TRANSPORT PROVISION IN SUCEAVA-BOTOȘANI URBAN AREA (ROMANIA

    Directory of Open Access Journals (Sweden)

    Marian Ionuț ISTRATE

    2016-06-01

    Full Text Available Having a major economic and social role, public transport is an important component in the process of sustainable development of a city. The paper aimed to study the public transport at metropolitan and regional scale evidencing how two major urban centers of Romania, Suceava and Botoșani, connect via public transport with the rural settlements in their metropolitan areas. The metropolitan supply of public transport was analysed starting with publicly available data on the routes in 2014 for both Botoșani County and Suceava County, located in the North-Eastern side of Romania. The routes’ spatial disposition and daily frequency is analysed for road and rail public transport. The resulted mappings showed that road public transport is well distributed in the metropolitan territory for both urban centres, but there is still almost 4% of the rural population that does not have direct access to this public utility service. The rail public transport covers only a small amount of the studied area and it has a secondary role in supplying public transport services for the population because of the lack of investments that the entire rail sector suffered in Romania. The share of private cars has increased significantly, but the population’s need for mobility is not completely covered and this paper offers some recommendations for the improvement of the public transportation supply in Suceava-Botoșani Urban Area.

  16. EVALUATION OF THE DNA POLYMORPHISM OF SIX VARIETIES OF CHENOPODIUM QUINOA WILLD, USING AFLP

    Directory of Open Access Journals (Sweden)

    Nolasco, Oscar

    2013-07-01

    Full Text Available The grain of Chenopodium quinoaWilld. (Quinua is important worldwide for its high nutritional value, being the principal source of proteins of the settlers of the Peruvian Bolivian altiplano. In the process of the care and maintenance of the species in the germplasm banks for quinua there have been programs developed to allow the evaluation of genetic variation to increase the quality of the grain, the resistance to diseases, and dryness tolerance and to modulate the content of saponins. A current question is to discriminate among the varieties using molecular sensitive technologies like the RAPDs, microsatellites, RFLP; our aim was to evaluate the polymorphism of six varieties of quinua using AFLP's technology (Amplified Fragment Length Polymorphism. Varieties of quinua: Quillahuaman INIA (Q, Mantaro (M, Hualhuas (H, Real Boliviana (B, Salcedo INIA(S and Illpa INIA(I, were evaluated in combinations of five pairs of primers using adapters for EcoRI and MseI to determine their polymorphisms. Our results found three combinations of major polymorphism E33/M60, E32/M48 and E32/M61, that we were able to discriminate against the variety Mantaro (M as a variety removed from the others of the southern Peruvian altiplano – Bolivian, according to UPGMAanalysis. This combination of primers also discriminate the varieties Illpa INIA and Salcedo INIA as being varieties obtained by crossings for genetic improvement.

  17. Cloning and Expression Analysis of TaLr19TLP1 Gene in Wheat Induced by Puc c inia tritic ina%叶锈菌诱导的小麦TaL r19TL P1基因的克隆及表达分析

    Institute of Scientific and Technical Information of China (English)

    栗小英; 高琳; 张艳俊; 王海燕; 刘大群

    2014-01-01

    为了探明类甜蛋白(thaumatin-like proteins, TLPs)基因与小麦抗叶锈病防御反应的相关性,本研究采用RT-PCR和电子克隆技术从叶锈菌侵染的TcLr19小麦叶片中获得了一个类甜蛋白基因,命名为TaLr19TLP1(GeneBank登录号:KJ764822)。生物信息学分析表明该基因包含一个长516 bp的开放阅读框(open reading frame, ORF),不含内含子,编码171个氨基酸残基,具有一个甜蛋白保守结构域(thaumatin, THN),氨基酸序列与其他物种类甜蛋白序列具有较高同源性。实时荧光定量PCR分析明确该基因表达受叶锈菌、脱落酸和水杨酸的诱导。接种叶锈菌后不同时间点,TaLr19TLP1基因在非亲和组合中表达早于亲和组合,且表达量明显高于亲和组合。另外,该基因表达具有组织特异性,在茎部表达量高于叶部和根部。Western杂交分析表明,TaLr19TLP1蛋白与小麦叶锈菌诱导具有相关性,与基因水平表达模式相一致。以上研究结果说明TaL r19TL P1基因参与小麦TcLr19小麦抗叶锈防御反应。%To understand the roles of thaumatin-like proteins (TLPs) gene in the response of wheat to leaf rust (Puccinia triticina) infection, one thaumatin-like proteins gene designated TaLr19TLP1 (GeneBank accession number: KJ764822) was obtained from TcLr19 wheat leaves infected by leaf rust pathogen using RT-PCR and silico cloning methods. Bioinformatics analysis showed that TaLr19TLP1 contained a single opening reading frame of 516 bp encoding 171 amino acid-peptides without intron, and the deduced amino acids sequence of TaLr19TLP1 has high homology with pathogenesis-related protein 5 obtained from other species with a thaumatin THN domain. The expression profiles of the TaLr19TLP1 gene detected by real-time quantitative PCR showed that TaLr19TLP1 gene could be induced by Puccinia triticina, abscisic acid (ABA) and salicylic acid (SA). TaLr19TLP1 was induced earlier and its expression level was higher in the incompatible interaction between wheat and Puccinia triticina than in compatible interactions. The tissue organ-specific expression pattern of the TaLr19TLP1 showed that its transcripts were detected abundantly in stems, and weakly in leaves and roots. Western blotting was conducted to confirm that TaLr19TLP1 protein was induced by Puccinia triticina infection which is similar to the expression profiles at the nucleotide level. All these results indicated that TaLr19TLP1 may play an important role in wheat resistance to leaf rust pathogen attack.

  18. Effect of reuterin-producing Lactobacillus reuteri coupled with glycerol on the volatile fraction, odour and aroma of semi-hard ewe milk cheese.

    Science.gov (United States)

    Gómez-Torres, Natalia; Ávila, Marta; Delgado, David; Garde, Sonia

    2016-09-02

    The effect of the biopreservation system formed by Lactobacillus reuteri INIA P572, a reuterin-producing strain, and glycerol (required for reuterin production), on the volatile fraction, aroma and odour of industrial sized semi-hard ewe milk cheese (Castellano type) was investigated over a 3-month ripening period. The volatile compounds were extracted and analyzed by SPME-GC-MS and cheese odour and aroma profiles were studied by descriptive sensory analysis. Control cheese was made only with a mesophilic starter and experimental cheeses with L. reuteri were made with and without glycerol. The addition of L. reuteri INIA P572 to milk enhanced the formation of six volatile compounds. Despite the changes in the volatile compounds profile, the use of L. reuteri INIA P572 did not noticeably affect the sensory characteristics of cheese. On the other hand, the addition of L. reuteri INIA P572 coupled with 30mM glycerol enhanced the formation of twelve volatile compounds, but decreased the formation of five ones. The use of the biopreservation system did not affect overall odour and aroma quality of cheese although it resulted in a significant decrease of the odour intensity scores. In addition, this cheese received significant higher scores for "cheesy" aroma and significant lower scores for the aroma attributes "milky", "caramel" and "yogurt-like". The first two axes of a principal component analysis (PCA) performed for selected volatile compounds and sensory characteristics, accounting for 75% of the variability between cheeses, separated cheeses made with L. reuteri INIA P572 and glycerol from the rest of cheeses, and also differentiated control cheese from cheeses made with L. reuteri INIA P572 from day 60 onward. Our results showed that the reuterin-producing L. reuteri INIA P572 strain, when coupled with glycerol, may be a suitable biopreservation system to use in cheese without affecting odour and aroma quality.

  19. Outgrowth inhibition of Clostridium beijerinckii spores by a bacteriocin-producing lactic culture in ovine milk cheese.

    Science.gov (United States)

    Garde, Sonia; Avila, Marta; Arias, Ramón; Gaya, Pilar; Nuñez, Manuel

    2011-10-17

    In the manufacture of model cheeses, ovine milk was deliberately contaminated with spores of Clostridium beijerinckii INIA 63, a wild isolate from Manchego cheese with late blowing defect, and inoculated with nisin- and lacticin 481-producing Lactococcus lactis subsp. lactis INIA 415 as starter, to test its potential to prevent the late blowing defect, or with L. lactis subsp. lactis INIA 415-2, a spontaneous mutant not producing bacteriocins. Cheeses made individually with the lactococcal strains, without clostridial spores, served as controls. Cheese made with clostridial spores and L. lactis subsp. lactis INIA 415-2 showed late blowing defect after 120days of ripening. Spoilt cheese also showed lower concentrations of lactic acid, and higher levels of acetic, propionic and butyric acids, and of other volatile compounds such as 2-propanol and 1-butanol, than control cheese. In addition, cheese made with the bacteriocin producer did not show any late blowing symptoms, despite its spore counts similar to those of blown cheese, pointing to outgrowth inhibition of C. beijerinckii spores by bacteriocins. Besides, cheese made with the bacteriocin producer showed similar concentrations of lactic acid and volatile compounds than control cheese. Inclusion of L. lactis subsp. lactis INIA 415 in starter cultures seems a feasible method to prevent late blowing defect in cheese without altering its sensory characteristics.

  20. Quality consumption of strawberries (Fragaria ananassa

    Directory of Open Access Journals (Sweden)

    Sergio Carballo

    2011-04-01

    Full Text Available Consumers increasingly require a food product that preserves their nutritional value; retain fresh colour, flavour and texture.Appearance, texture and flavour are attributes identified as to be key to acceptability of fruit and also for consumers in making purchase of fresh produce.The objective of this work was to investigate the consumer's preference of sensory quality and the commercial aspects of fresh strawberries, harvested in spring time from the south of our country, Uruguay- South America.In this study, seven different strawberries varieties (INIA-Arazá, INIA- Ivapitá, Ventana, Seascape, Camarosa, INIA- Ivahé, Aromas were sensory and physicochemical evaluated.The results obtained showed a high correlation between total soluble solids and sensory attributes (colour, texture, and flavour.Camarosa and Ivahé varieties were prefered for the external and internal aspects of the fruit.Future work should investigate other producer areas of the country and other harvesting time.

  1. Aspectos histológicos do trato digestivo de Sotalia fluviatilis (Cetacea, Delphinidae: esôfago e estômago

    Directory of Open Access Journals (Sweden)

    Paulo de Tarso da C. Chaves

    1988-07-01

    Full Text Available Os esôfagos e estômagos de seis botos tucuxi, Sotalia fluviatilis (Cetacea: Delphinidae foram examinados anatômica e microscopicamente e comparados com os de botos marinhos do mesmo gênero. O esôfago é um simples tubo distensível e macroscopicamente uniforme até sua junção com o estômago. Este boto tem um estômago tricompartimentado: uma parte anterior ou muscular; uma principal, glandular; e uma pilórica. As características anatomo-funcionais de cada compartimento são descritas. Não se achou esfíncter entre o esôfago e o estômago. Em comparação com os delfinídeos marinhos, não há adaptações, por parte de Sotalia, à invasão relativamente recente do ambiente de água doce.The oesophagus and stomachs of six tucuxi dolphins, Sotalia fluviatilis (Cetacea: Delphinidae were examined anatomically and microscopically and compared with marine dolphins of the same genus. The oesophagus is a simple distendable tube and is macroscopically uniform to its junctureh with te stomach. This dolphin has a three-compartmented stomach: an anterior or muscular compartment; a principal one, which is glandular; and the pyloric part. The anatomical and functional characteristics of each compartment is described. No sphincter was found between the oesophagus and the stomach. In comparison with marine delphinids there are no adaptations specific to the relatively recent invasion of the fresh-water environment by Sotalia.

  2. Las concepciones del "Ius naturale" o los fundamentos de la política en Grocio, Hobbes y Espinosa

    OpenAIRE

    Blanco Echauri, Jesús (Ed.)

    2003-01-01

    El estudio de los textos del jurista Huigh de Groot revela la gestación y puesta a punen de un formidable dispositivo jurídico que ha ejercido una notable influencia histórica sobre botos parte de los mayores filósofos políticos de su siglo. Tanto Hobbes como Espinosa reconducen a la unidad los dos facultades originales que, situadas sobre un mismo plano, Grocio se limitaba a concebir en términos de simple yuxtaposición, repensando la relación entre la propiedad (dominium) de la persona físic...

  3. Python and AWS Cookbook

    CERN Document Server

    Garnaat, Mitch

    2011-01-01

    If you intend to use Amazon Web Services (AWS) for remote computing and storage, Python is an ideal programming language for developing applications and controlling your cloud-based infrastructure. This cookbook gets you started with more than two dozen recipes for using Python with AWS, based on the author's boto library. You'll find detailed recipes for working with the S3 storage service as well as EC2, the service that lets you design and build cloud applications. Each recipe includes a code solution you can use immediately, along with a discussion of why and how the recipe works. You al

  4. A presença do judeu em Contos Amazônicos, de Inglês de Sousa

    Directory of Open Access Journals (Sweden)

    Deuziane de Vasconcelos Barbosa

    2014-11-01

    Full Text Available Este artigo analisará a relação entre o personagem judeu e a lenda do boto, presente no conto “O baile do judeu”, inserido em Contos amazônicos, de Inglês de Sousa. A princípio, são trazidas informações sobre a vida e obra do autor, além de uma pesquisa da recepção especializada acerca de sua carreira literária. Em seguida, as atenções são voltadas para os elementos principais da análise, o judeu, que consiste na apresentação da história do povo semita desde sua origem, passando pelo período de migração para a Amazônia, até os dias de hoje, abordando o antissemitismo, costumes e o imaginário construído sobre esses imigrantes. Seguindo com uma breve abordagem sobre o imaginário amazônico voltado para a lenda do boto. Como última etapa deste trabalho, traz-se a análise do conto “O baile do judeu”, atentando para o imaginário amazônico, imaginário a cerca dos judeus e o antissemitismo presente na narrativa.

  5. Fish Vaccine Development and Use to Prevent Streptococcal Diseases

    Science.gov (United States)

    An important pathogen of tilapia, hybrid striped bass and trout raised in intensive aquaculture is Streptococcus sp., a cause of severe economic losses in the fish farming industry. Infected fish experience severe to moderate mortality due to Streptococcus iniae and/or S. agalactiae. The diseased ...

  6. Bioassay-directed isolation and evaluation of Harmine from the terrestrial plant Peganum harmala L. for antibacterial activity against Flavobacterium columnare

    Science.gov (United States)

    The antibacterial activities of crude extracts obtained from the aerial portions and roots of Peganum harmala L. were evaluated against the common fish pathogenic bacteria species Edwardsiella ictaluri, Flavobacterium columnare, and Streptococcus iniae using a rapid bioassay. Enteric septicemia of c...

  7. 硝唑尼特--一种新的抗原虫药

    Institute of Scientific and Technical Information of China (English)

    陈阜新; 韩洪刚

    2004-01-01

    @@ 硝唑尼特(nitazoxanid,商品名A1inia)是一种新的抗原虫药,由美国FDA批准其液体制剂治疗隐孢子虫病(Crytosporidium parvum)和肠贾第虫(Giardia lamblia)所致1~11岁儿童的腹泻.

  8. ISOLASI BAKTERIOFAGA ANTI Streptococcus agalactiae DARI IKAN NILA (Oreochromis niloticus

    Directory of Open Access Journals (Sweden)

    Angela Mariana Lusiastuti

    2016-11-01

    Full Text Available Infeksi Streptococcus merupakan salah satu penyakit serius pada ikan yang disebabkan oleh bakteri gram positif. Infeksi oleh streptococcus beta-hemolitik paling sering dilaporkan menginfeksi ikan. Di antara streptococci beta-hemolitik, Streptococcus iniae penyebab septicemia, meningoencefalitis, dan kematian pada ikan budidaya. Selain itu, Streptococcus agalactiae juga menyebabkan streptococcosis parah pada ikan nila. Alternatif yang bisa digunakan untuk terapi infeksi streptococcosis adalah dengan penggunaan bakteriofaga yang merupakan virus yang hidup pada bakteri. Tujuan penelitian ini adalah isolasi bakteriofaga S. agalactiae sebagai kandidat agen terapi yang memberikan efek protektif melawan infeksi streptococcosis. Faga diisolasi dari Brain Heart Infusion Agar (BHIA yang sudah ditanami dengan 15 isolat S. iniae dan S. agalactiae. Isolat S. iniae dan S. agalactiae diisolasi dari ikan sakit dengan gejala klinis Streptococcosis. Setelah itu diidentifikasi dengan pewarnaan Gram, tes katalase, pertumbuhan pada agar darah dan API 20 Strep System. Pertumbuhan faga ditunjukkan dengan adanya zona lisis pada tempat yang ditetesi dengan sampel cairan usus dari ikan nila sehat. Faga yang tumbuh lalu dikoleksi secara steril, disentrifus dan supernatannya difiltrasi dengan membran filter 0,45 µm dan disimpan pada suhu 4oC. Dari 15 isolat S. iniae dan S. agalactiae hanya satu isolat yaitu PSaT-18 yang menunjukkan zona lisis seperti yang ditunjukkan pada cawan petri isolat kontrol S. iniae. Zona lisis tersebut timbul akibat adanya faga yang memberikan proteksi terhadap S. iniae dan S. agalactiae. Zona lisis yang tidak jernih disebabkan konsentrasi faga yang terlalu rendah akibat dilakukan pengenceran pada proses filtrasi. Faga yang diperoleh selanjutnya akan dilakukan uji in vitro dan in vivo untuk mengetahui efektivitasnya. Streptococcal infection is a serious disease in fish caused by gram positive bacteria. The causative agent is Streptococcus b

  9. Estrés-recuperación en deportistas y su relación con los estados de ánimo y las estrategias de afrontamiento

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    Olga Molinero

    2012-01-01

    Full Text Available El objetivo del estudio es evaluar situaciones relacionadas con el sobreentrenamiento (RESTQ-Sport, Kellmann y Kallus, 2001; González- Boto et al., 2008c en el contexto deportivo, e identificar su relación temporal con las modificaciones en los estados de ánimo (POMS, McNair y cols.,1971; Balaguer et al. 1993 y la posible influencia en la aparición de desequilibrios entre estrés y recuperación en función del tipo de estrategias de afrontamiento utilizadas (ISCCS, Gaudreau y Blondin, 2002; Molinero et al., 2006, 2008, 2009. Han participado 167 deportistas (62.5% hombres y 36.9% mujeres entre 18 y 24 años pertenecientes a 11 modalidades deportivas. Los resultados muestran que se producen modificaciones a los largo de las tres tomas realizadas en los niveles de estrés general (p = .027*, conflictospresión (p = .000**, fatiga (p = .000**, bienestar general (p = .022*, burnout-fatigaemocional(p = .009**, forma física-lesiones (p = .000**, burnout- realización personal (p = .000**, autorregulación (p = .000**, Estrés no deportivo (p = .006**, Estrés y recuperación deportiva (p = .001**; p= .000**, Estrés Total (p = .001** en relación a los índices medidos por el RESTQ Sport. Anivel emocional observamos cambios en los niveles de depresión (p = .040*, cólera, (p = .006**, confusión (p = 036* y Alteración EmocionalTotal (p = .041*, y no se producen cambios en el uso de las distintas estrategias de afrontamiento. Es necesario prestar más atención a los cambiostemporales durante la recuperación, que pueden inducir adaptaciones positivas tras periodos de sobreentrenamiento, y su relación con los estados de ánimo de los deportistas, que han sido utilizados habitualmente como indicadores de fatiga (González- Boto et al., 2009; Kellmann et al., 2001; Rietjens et al., 2005. Los deportistas utilizan una serie de estrategias de afrontamiento que podrían condicionar de forma diversa el balance entre estrés y recuperación y, por

  10. Behavioral patterns and movement intensity of Sotalia guianensis (P. J. van Bénéden (Cetacea, Delphinidae in two different areas on the Brazilian coast Padrões comportamentais e intensidade de movimentos de Sotalia guianensis (P. J. van Bénéden (Cetacea, Delphinidae em duas diferentes áreas da costa brasileira

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    Fábio G. Daura-Jorge

    2007-06-01

    Full Text Available The behavioral patterns of the estuarine dolphin, Sotalia guianensis (P. J. van Bénéden, 1864, were compared between two populations along the Brazilian coast: Caravelas (Bahia, along the eastern coast, and Norte Bay (Santa Catarina, along the southern coast. Applying the focal-group sampling in both areas, information such as the geographic position (UTM of groups and predominant behavioral patterns were obtained. Geographic positions were used to calculate the total distance traveled by estuarine dolphin groups on each observation day. Since the distance traveled varies with time, the daily mean speed of the dolphin’s group was used as an index of movement intensity. Two comparable and easily recognizable behavioral patterns were considered: travelling and foraging. Overall movement intensity and behavioral pattern frequency were similar between both areas. However, a seasonal variation was observed in both parameters in Norte Bay, while in Caravelas these parameters were homogeneous throughout the year. Variation in the behavior of the estuarine dolphin was consistent with variations in environmental factors, such as water temperature.Os padrões comportamentais do botocinza, Sotalia guianensis (P. J. van Bénéden, 1864, foram comparados entre duas populações da costa brasileira: Caravelas (Bahia, na costa leste, e Baía Norte (Santa Catarina, na costa sul. Utilizando o método de grupo-focal em ambas as áreas, informações como posições geográficas (UTM do grupo e padrão comportamental predominante foram coletadas. A posição geográfica foi utilizada para o cálculo da distância total percorrida por um grupo de botos-cinza em cada área de observação. Como a distância é uma medida que varia com o tempo, a velocidade média diária do grupo de botos foi usada como um índice de intensidade de movimento. Dois padrões comportamentais comparáveis e facilmente reconhecíveis foram considerados: deslocamento e forrageamento

  11. Molecular epidemiology of zoonotic streptococcosis/lactococcosis in rainbow trout (Oncorhynchus mykiss aquaculture in Iran

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    G Nikbakhat-Brojeni

    2010-12-01

    Full Text Available Background and Objectives: Streptococcosis/lactococcosis is a hyperacute systemic disease that can occur in marine and fresh waters of many species of fish. The aim of this work was to study the disease outbreak in the major rainbow trout (Oncorhynchus mykiss production of Iran."nMaterials and Methods: 108 Gram positive cocci isolates were obtained from diseased trout in seven provinces with major trout production during 2008 till 2009. These bacterial isolates were characterized using phenotypic and molecular studies. The isolates were also analysed phylogeneticaly and compared with the available data."nResults: 49 samples (45.37% were identified as Streptococcus iniae, 37 samples (35.2% matched with Lactococcus garvieae; and 22 samples (19.43% were identified as members of Streptooccus genus by culture-based and biochemical tests of API 50 CH, API 20 STREP and rapid 32 STREP systems. Using universal primers for differentiation of Streptococcus sp. and Enterococcus sp, all 108 samples were identified as Streptococcus sp. with a target region of 500 bp. Single specific PCR resulted in identification of 64 (59.2% isolates as S. iniae and 44 (40.8% isolates as L. garvieae. The phylogenetic analysis of the S. iniae isolates resulted in maximal similarity to some strains reported from Taiwan and to all Brazilian strains. Also, one strain showed less sequence similarity values with other tested strains although this strain has high similarity with ATCC 29178 strain, all reported Chinese, and some Taiwanian strains. Also, analysis of S. iniae LctO gene sequence showed that this isolate clustered within the S. iniae group. The sequence analysis of L. garvieae strains also showed that they have maximum similarity to all Japanese and Chinese strains, but one strain has lower sequence similarity values with all other recorded strains."nConculsion: The results of this study clearly show that trout farming in Iran is severely affected by both species of S

  12. Variação osteológica e desenvolvimento ontogenético das espécies do gênero Sotalia (Cetacea, Delphinidae)

    OpenAIRE

    Fettuccia, Daniela de Castro

    2010-01-01

    Apesar da existência de importantes trabalhos morfológicos para as duas espécies do gênero Sotalia, é limitado o conhecimento do esqueleto como um todo, considerando a variação ontogenética inter e intraespecífica, geográfica e sexual. Com o intuito de contribuir para um melhor conhecimento osteológico e fornecer subsídios para a conservação foram avaliados 536 sincrânios e 257 pós-crânios parciais e completos de S. guianensis (boto-cinza) de várias localidades da América do...

  13. [Folklore and popular medicine in the Amazon].

    Science.gov (United States)

    Henrique, Márcio Couto

    2009-01-01

    This discussion of the relations between folklore and popular medicine in the Amazon takes Canuto Azevedo's story "Filhos do boto" (Children of the porpoise) as an analytical reference point. Replete with elements of cultural reality, folk tales can serve as historical testimonies expressing clashes between different traditions. Folk records are fruit of what is often a quarrelsome dialogue between folklorists, social scientists, physicians, and pajés and their followers, and their analysis should take into account the conditions under which they were produced. Based on the imaginary attached to the figure of the porpoise--a seductive creature with healing powers--the article explores how we might expand knowledge of popular medicine as practiced in the Amazon, where the shamanistic rite known as pajelança cabocla has a strong presence.

  14. Isolation and characterization of bacteria with antibacterial properties from Nile tilapia (Oreochromis niloticus).

    Science.gov (United States)

    Etyemez, Miray; Balcazar, Jose Luis

    2016-04-01

    One hundred and twenty bacterial isolates were obtained from the intestinal mucus of Nile tilapia (Oreochromis niloticus) and screened for antagonistic activity and adherence abilities. Based on in vitro antagonism against two pathogens (Streptococcus iniae and Edwardsiella piscicida), five isolates were selected and identified by 16S rRNA gene sequence analysis. All antagonistic isolates were affiliated to the genus Bacillus, which showed inhibitory activity against S. iniae. Only the isolate B191 (closely related to Bacillus mojavensis) inhibited the growth of both pathogens. Moreover, isolate B191 adhered significantly better to fish intestinal mucus than other antagonistic isolates. According to our results, these bacterial isolates, particularly isolate B191, should be further studied to explore their probiotic effects under in vivo conditions.

  15. Exploring Early Detection Methods: Using the Intraductal Approach to Predict Breast Cancer

    Science.gov (United States)

    2005-06-01

    mainan ~t (ancer or in situ) or in situ) _____, 9=uncertain 9-__ _ __9uncertain If you had more than 5 biopsies, please write the information in...assessment of experienced risk counselors (Euhus, Smith , avaehs it mo st ipra inia applca tionet al., 2002). assessment tool and is best used in a breast...estrogen levels in healthy Finn- Euhus, D.M., Smith , K.C., Robinson, L., Stucky, A., Olopade, 0.1., Cum- ish and American women and patients with

  16. Exploration of the Reputation of the Nightclub Industry in Ireland

    OpenAIRE

    Friel, Anthony

    2013-01-01

    The purpose of the exploration was to research the reputation and public relations practised within the nightclub industry. This is achieved through exploring the public relations practices of the Irish Nightclub Industry Association (INIA) and individual nightclubs. The specific areas examined are public relations as a management function, communications, relationship management, an understanding of publics, issue management and crisis management. Reputation and the reputation management pro...

  17. Characterization and identification of streptococci from golden pompano in China.

    Science.gov (United States)

    Cai, X H; Peng, Y H; Wang, Z C; Huang, T; Xiong, X Y; Huang, Y C; Wang, B; Xu, L W; Wu, Z H

    2016-05-26

    Streptococcal infections cause significant mortality and high economic losses in the fish farm industry worldwide, including in the culture of golden pompano Trachinotus ovatus L., a species gaining popularity in China. A total of 9 streptococcal strains were isolated from cage-cultured diseased golden pompano in Beihai, Zhanjing, and Shenzhen, China, between 2012 and 2014. Conventional and rapid identification systems were used to determine that the isolates were Streptococcus agalactiae, S. iniae, and S. dysgalactiae subsp. dysgalactiae. All isolates were gram-positive cocci cells in pairs or short-chain, non-motile, catalase negative, α or β hemolytic cocci. The results of multiplex PCR assays and 16S rRNA BLAST analysis also showed that the β hemolytic strains were S. agalactiae and S. iniae and the α hemolytic strain was S. dysgalactiae subsp. dysgalactiae, respectively. Pathogenicity assays revealed that S. agalactiae (lethal dose [LD50]: 6.38 × 10(4) CFU ml(-1)) was more virulent for golden pompano than S. iniae (LD50: 1.47 × 10(7) CFU ml(-1)) and S. dysgalactiae subsp. dysgalactiae (LD50: 2.57 × 10(6) CFU ml(-1)) when they were challenged by intraperiotoneal (i.p.) injection. The results of antibiotic susceptibility showed that all strains were extremely susceptible to cefradine, erythromycin, and cefotaxime but resistant to gentamicin, penicillin G, novobiocin, neomycin, ciprofloxacin, roxithromycin, furazolidone, enrofloxacin, norfloxacin, kanamycin, ampicillin, tetracycline, and vancomycin This is the first report of a phenomenon of golden pompano coinfection with S. agalactiae and S. iniae, which will contribute to the diagnosis and prevention of streptococcicosis.

  18. Growth and functional responses of different cultivars of Lotus corniculatus to aluminum and low pH stress.

    Science.gov (United States)

    Pavlovkin, Ján; Pal'ove-Balang, Peter; Kolarovic, Lukás; Zelinová, Veronika

    2009-09-15

    Aluminum toxicity is an important stress factor in acid soils. Growth, respiration and permeability properties of root cells were studied in five cultivars of Lotus corniculatus subjected to aluminum (Al) or low pH stress. The cultivars showed significant differences in root elongation under stress conditions, which correlated with changes in membrane potential (E(M)) of root cortical cells. A pH drop from 5.5 to 4.0 resulted in significant membrane depolarization and root growth inhibition. The strongest inhibition was observed in cv. São Gabriel (33.6%) and least in cv. UFRGS (25.8%). Application of an extremely high Al concentration (2mM) stopped the root growth in cv. INIA Draco, while inhibition in cv. UFRGS reached only 75%. The E(M) values of cortical cells of Lotus roots varied between -115 and -144mV. Treatment with 250microM of AlCl(3) (pH 4) resulted in rapid membrane depolarization. The extent of the membrane depolarization ranged between 51mV (cv. UFGRS) and 16mV (cv. INIA Draco). The membrane depolarization was followed by a loss of K(+) from Al-treated roots (2mM Al) and resulted in a decrease of the diffusion potential (E(D)). The total amount of K(+) in Al-treated roots dropped from 31.4 to 16.8micromolg(-1) FW in sensitive cv. INIA Draco, or from 26.1 to 22.7micromolg(-1) FW in tolerant cv. UFGRS. The rate of root respiration under control conditions as well as under Al treatment was higher in cv. INIA Draco than in cv. UFRGS. Al-induced inhibition of root respiration was 21-34% of the control.

  19. Cold tolerance evaluation in Chilean rice genotypes at the germination stage

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    Gabriel Donoso Ñanculao

    2013-03-01

    Full Text Available Low temperature is the most important abiotic stress affecting rice (Oryza sativa L. yield in Chile. Rice in Chile is usually planted when the minimum air temperatures are below 12 °C. This temperature is lower than the optimum needed for normal rice germination. Therefore, the aim of this study was to evaluate cold tolerance in 20 experimental lines from the Rice Breeding Program of the Instituto de Investigaciones Agropecuarias (INIA, Chile, at the germination stage. Coleoptile length reduction (CRED, coleoptile length after cold treatment (CLEN, coleoptile length recovery (CREC, and coleoptile regrowth (CREG were evaluated at 13 °C for 4 d using 'Diamante-INIA' as the cold-tolerant control. To find genotypes with cold tolerance (low CRED value and high CLEN, CREC, and CREG values, genotypes were ranked, a biplot of principal components, and cluster analysis were performed. No differences were found among genotypes in the ranking based on CREC value so this trait was not considered. Analysis showed that only three experimental lines had cold tolerance similar to that of 'Diamante-INIA'; all other experimental lines exhibited intermediate to low cold tolerance. These results showed low cold tolerance of some Chilean genotypes at the germination stage, thus confirming the need to evaluate the rest of the germplasm from the Rice Breeding Program.

  20. Guiana dolphins (Sotalia guianensis displaying beach hunting behavior in the Cananéia Estuary, Brazil: social context and conservation issues

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    Marcos César de Oliveira Santos

    2010-06-01

    procura e captura de alimento em praias (“beach hunting” exibido por botos-cinza (Sotalia guianensis no Estuário de Cananéia (EC (25ºS, 48ºW, um estudo baseado na aplicação da técnica de foto-identificação foi conduzido de maio de 2000 a julho de 2003. Três plataformas de observação a foram escolhidas na principal entrada do mencionado estuário, onde os pesquisadores encontram uma oportunidade ímpar de observar os botos a uma pequena distância sem incomodá-los. As observações foram oportunísticas e desigualmente distribuídas ao longo do tempo e do espaço. Adicionadas às observações de identificação individual, uma balestra de 80lbs de pressão foi utilizada para coletar amostras de pele de oito indivíduos monitorados com vistas à determinação do sexo. Em 67 dias de investigação, que renderam aproximadamente 80h de observações diretas dos botos, 4.102 fotografias foram tomadas, das quais 1.098 (26,8% foram consideradas úteis para o propósito de identificação individual. Um total de 103 grupos foi observado (3,7 ± 2,6 indivíduos, variando entre botos solitários e 15 indivíduos. Pares de fêmeas e filhotes foram observados em 92,4% dos grupos investigados. Apresenta-se a primeira notificação de intervalo de nascimento de um filhote para o EC: aproximadamente 3 anos e 9 meses. De 40 indivíduos identificados ao menos uma vez, onze usaram regularmente as praias: fêmeas KN #s 10, 30, 255, 268 e 279; machos KN #s 86 e 257; e quatro indivíduos de sexo desconhecido. As características das associações entre os indivíduos monitorados foram avaliadas através das análises de índices de associação. Um total de 83 associações possíveis entre pares de botos foi analisado utilizando o índice de peso médio e dois critérios de seleção (5+ e 8+ avistamentos/indivíduo. A média dos índices de associação variou entre 0,12 e 0,16 evidenciando laços fracos entre os indivíduos monitorados, cuja maioria foi composta por f

  1. The behavior of Sotalia guianensis (Van Bénéden in Pernambuco coastal waters, Brazil, and a further analysis of its reaction to boat traffic O comportamento de Sotalia guianensis (Van Bénéden em águas costeiras de Pernambuco, Brasil, e uma análise adicional de sua reação ao tráfego de embarcações

    Directory of Open Access Journals (Sweden)

    Janaina P. Araújo

    2008-03-01

    Full Text Available There is still little knowledge about the behavior of the estuarine dolphins and also the way they react to the presence of different types of vessels. The aim of the present study was twofold: to investigate the behavior of estuarine dolphins in four locations in Pernambuco, Brazil, and also their reaction to the presence of common types of nautical crafts. The data of this study resulted from 48 fieldtrips to each of the four localities studied (ports of Recife and Suape, and beaches of Bairro Novo and Piedade, thus totaling 192 days of observations, with approximately 380 hours of actual sampling. Ten types of behavioral activities were counted for Bairro Novo beach and the port areas, but only four for Piedade beach. The greatest flows of boats were recorded in the port of Recife. Fishing boats were the commonest type in the port area of Recife and Bairro Novo beach, while tourist vessels predominated in the port of Suape and Piedade beach. During the observations of encounters between these boats and the estuarine dolphins, neutral reactions predominated for all the vessel types studied. The same type of reaction predominated when the distances at which the interactions between the animals and vessels occurred were correlated.Ainda se sabe pouco sobre o comportamento do boto-cinza, assim como a forma pela qual ele reage a diferentes tipos de embarcações. O presente estudo teve dois objetivos: investigar o comportamento dos botos-cinzas em quatro localidades de Pernambuco, Brasil, assim como a reação destes para com as embarcações náuticas comuns. Os dados foram coletados no período de setembro de 2004 a agosto de 2006, no horário das 6 às 17 h. Os dados desta pesquisa resultaram de 48 saídas a campo para cada uma das quatro localidades estudadas (Portos do Recife e Suape, praia de Bairro Novo e Piedade, totalizando 192 dias de observações, sendo aproximadamente 380 horas de amostragens efetivas. Foram contados dez tipos de

  2. Evaluation of reference genes for quantitative real-time RT-PCR analysis of gene expression in Nile tilapia (Oreochromis niloticus).

    Science.gov (United States)

    Yang, Chang Geng; Wang, Xian Li; Tian, Juan; Liu, Wei; Wu, Fan; Jiang, Ming; Wen, Hua

    2013-09-15

    Quantitative real-time reverse-transcriptase polymerase chain reaction (RT-qPCR) has been used frequently to study gene expression related to fish immunology. In such studies, a stable reference gene should be selected to correct the expression of the target gene. In this study, seven candidate reference genes (glyceraldehyde-3-phosphate dehydrogenase (GADPH), ubiquitin-conjugating enzyme (UBCE), 18S ribosomal RNA (18S rRNA), beta-2-microglobulin (B2M), elongation factor 1 alpha (EF1A), tubulin alpha chain-like (TUBA) and beta actin (ACTB)), were selected to analyze their stability and normalization in seven tissues (liver, spleen, kidney, brain, heart, muscle and intestine) of Nile tilapia (Oreochromis niloticus) challenged with Streptococcus agalactiae or Streptococcus iniae, respectively. The results showed that all the candidate reference genes exhibited tissue-dependent transcriptional variations. With PBS injection as a control, UBCE was the most stable and suitable single reference gene in the intestine, liver, brain, kidney, and spleen after S. iniae infection, and in the liver, kidney, and spleen after S. agalactiae infection. EF1A was the most suitable in heart and muscle after S. iniae or S. agalactiae infection. GADPH was the most suitable gene in intestine and brain after S. agalactiae infection. In normal conditions, UBCE and 18S rRNA were the most stably expressed genes across the various tissues. These results showed that for RT-qPCR analysis of tilapia, selecting two or more reference genes may be more suitable for cross-tissue analysis of gene expression.

  3. Antibacterial Activities of Metabolites from Platanus occidentalis (American sycamore) against Fish Pathogenic Bacteria

    Science.gov (United States)

    Schrader, Kevin K; Hamann, Mark T; McChesney, James D; Rodenburg, Douglas L; Ibrahim, Mohamed A

    2016-01-01

    One approach to the management of common fish diseases in aquaculture is the use of antibiotic-laden feed. However, there are public concerns about the use of antibiotics in agriculture and the potential development of antibiotic resistant bacteria. Therefore, the discovery of other environmentally safe natural compounds as alternatives to antibiotics would benefit the aquaculture industries. Four natural compounds, commonly called platanosides, [kaempferol 3-O-α-L-(2″,3″-di-E-p-coumaroyl)rhamnoside (1), kaempferol 3-O-α-L-(2″-E-p-coumaroyl-3″-Z-p-coumaroyl)rhamnoside (2), kaempferol 3-O-α-L-(2″-Z-p-coumaroyl-3″-E-p-coumaroyl)rhamnoside (3), and kaempferol 3-O-α-L-(2″,3″-di-Z-p-coumaroyl)rhamnoside (4)] isolated from the leaves of the American sycamore (Platanus occidentalis) tree were evaluated using a rapid bioassay for their antibacterial activities against common fish pathogenic bacteria including Flavobacterium columnare, Edwardsiella ictaluri, Aeromonas hydrophila, and Streptococcus iniae. The four isomers and a mixture of all four isomers were strongly antibacterial against isolates of F. columnare and S. iniae. Against F. columnare ALM-00-173, 3 and 4 showed the strongest antibacterial activities, with 24-h 50% inhibition concentration (IC50) values of 2.13 ± 0.11 and 2.62 ± 0.23 mg/L, respectively. Against S. iniae LA94-426, 4 had the strongest antibacterial activity, with 24-h IC50 of 1.87 ± 0.23 mg/L. Neither a mixture of the isomers nor any of the individual isomers were antibacterial against isolates of E. ictaluri and A. hydrophila at the test concentrations used in the study. Several of the isomers appear promising for the potential management of columnaris disease and streptococcosis in fish.

  4. Isthminia panamensis, a new fossil inioid (Mammalia, Cetacea from the Chagres Formation of Panama and the evolution of ‘river dolphins’ in the Americas

    Directory of Open Access Journals (Sweden)

    Nicholas D. Pyenson

    2015-09-01

    Full Text Available In contrast to dominant mode of ecological transition in the evolution of marine mammals, different lineages of toothed whales (Odontoceti have repeatedly invaded freshwater ecosystems during the Cenozoic era. The so-called ‘river dolphins’ are now recognized as independent lineages that converged on similar morphological specializations (e.g., longirostry. In South America, the two endemic ‘river dolphin’ lineages form a clade (Inioidea, with closely related fossil inioids from marine rock units in the South Pacific and North Atlantic oceans. Here we describe a new genus and species of fossil inioid, Isthminia panamensis, gen. et sp. nov. from the late Miocene of Panama. The type and only known specimen consists of a partial skull, mandibles, isolated teeth, a right scapula, and carpal elements recovered from the Piña Facies of the Chagres Formation, along the Caribbean coast of Panama. Sedimentological and associated fauna from the Piña Facies point to fully marine conditions with high planktonic productivity about 6.1–5.8 million years ago (Messinian, pre-dating the final closure of the Isthmus of Panama. Along with ecomorphological data, we propose that Isthminia was primarily a marine inhabitant, similar to modern oceanic delphinoids. Phylogenetic analysis of fossil and living inioids, including new codings for Ischyrorhynchus, an enigmatic taxon from the late Miocene of Argentina, places Isthminia as the sister taxon to Inia, in a broader clade that includes Ischyrorhynchus and Meherrinia, a North American fossil inioid. This phylogenetic hypothesis complicates the possible scenarios for the freshwater invasion of the Amazon River system by stem relatives of Inia, but it remains consistent with a broader marine ancestry for Inioidea. Based on the fossil record of this group, along with Isthminia, we propose that a marine ancestor of Inia invaded Amazonia during late Miocene eustatic sea-level highs.

  5. Isthminia panamensis, a new fossil inioid (Mammalia, Cetacea) from the Chagres Formation of Panama and the evolution of 'river dolphins' in the Americas.

    Science.gov (United States)

    Pyenson, Nicholas D; Vélez-Juarbe, Jorge; Gutstein, Carolina S; Little, Holly; Vigil, Dioselina; O'Dea, Aaron

    2015-01-01

    In contrast to dominant mode of ecological transition in the evolution of marine mammals, different lineages of toothed whales (Odontoceti) have repeatedly invaded freshwater ecosystems during the Cenozoic era. The so-called 'river dolphins' are now recognized as independent lineages that converged on similar morphological specializations (e.g., longirostry). In South America, the two endemic 'river dolphin' lineages form a clade (Inioidea), with closely related fossil inioids from marine rock units in the South Pacific and North Atlantic oceans. Here we describe a new genus and species of fossil inioid, Isthminia panamensis, gen. et sp. nov. from the late Miocene of Panama. The type and only known specimen consists of a partial skull, mandibles, isolated teeth, a right scapula, and carpal elements recovered from the Piña Facies of the Chagres Formation, along the Caribbean coast of Panama. Sedimentological and associated fauna from the Piña Facies point to fully marine conditions with high planktonic productivity about 6.1-5.8 million years ago (Messinian), pre-dating the final closure of the Isthmus of Panama. Along with ecomorphological data, we propose that Isthminia was primarily a marine inhabitant, similar to modern oceanic delphinoids. Phylogenetic analysis of fossil and living inioids, including new codings for Ischyrorhynchus, an enigmatic taxon from the late Miocene of Argentina, places Isthminia as the sister taxon to Inia, in a broader clade that includes Ischyrorhynchus and Meherrinia, a North American fossil inioid. This phylogenetic hypothesis complicates the possible scenarios for the freshwater invasion of the Amazon River system by stem relatives of Inia, but it remains consistent with a broader marine ancestry for Inioidea. Based on the fossil record of this group, along with Isthminia, we propose that a marine ancestor of Inia invaded Amazonia during late Miocene eustatic sea-level highs.

  6. Evidence of vertical transmission and tissue tropism of Streptococcosis from naturally infected red tilapia (Oreochromis spp.

    Directory of Open Access Journals (Sweden)

    Padmaja Jayaprasad Pradeep

    2016-05-01

    Full Text Available Streptococcosis is a highly problematic disease in the aquaculture of freshwater fishes, especially for tilapia. The possibility of vertical transmission of streptococcosis and the pattern of tissue tropism of this pathogen in various organs was examined in red tilapia (Oreochromis sp.. Healthy broodstock without any clinical signs of Streptococcus spp. were selected from a farm earlier reported to have the disease and a total of 10 pairs were forced spawned to provide samples of gametes and progeny for pathogen testing. A colorimetric LAMP assay was used to confirm whether the bacterial pathogens Streptococcus. agalactiae and Streptococcus. iniae was present in samples of milt, unfertilized eggs, fertilized eggs, and offspring at various stages of development, as well as internal organs of broodstock (reproductive organs, gill, liver, spleen, kidney and brain as well as samples of water from culture systems. The majority of samples of milt (9/10 and unfertilized eggs (7/10 collected from the broodstock were infected with S. iniae at the time of spawning and was transmitted to all of their offspring. Nevertheless, when the same samples of gametes were analyzed for S. agalactiae, they were all found to be negative but the pathogen was found to be present in some 10-day-old larval offspring (4/10. However, when the pathogenic presence was analyzed from the reproductive organs of the parents, both S. agalactiae (11/20 and S. iniae (18/20 bacterium were common. Although, all broodstock were asymptomatic, almost all broodstock harboured the bacteria in many organs. Confirmation of vertical transmission of streptococcosis in tilapia means that intergenerational break cannot be used as a reliable and simple means of reducing or eliminating the prevalence of these difficult pathogens in aquaculture stock.

  7. Catalog of crater lakes from Costa Rica

    Science.gov (United States)

    Ramirez, C. J.; Mora-Amador, R.; González, G.

    2010-12-01

    Costa Rica has a diversity of volcanic crater lakes that can be classified into two groups: hot and cold lakes. The country contains at least 5% of the world's hot lakes. Costa Rica has 2 hot hyperacidic lakes, both of them on active volcanoes, the Rincón de la Vieja (38.0°C, pH = 0 - 1) and the Poás Laguna Caliente (36.1°C - 56°C, pH = 0.55 - 0.74), nowadays the Poás hot lake is the most active crater lake in the world, with more than 200 eruptions only on 2010. One of the most studied cold crater lakes is Irazú (13°C, pH = 3.5), that used to contain bubbling and clear areas of upwelling involving CO2 liberation and subaqueous fumaroles with temperatures up to 50°C, but since 2005 the lake presents an important descend until April 2010 when it disappeared. On February 9, 2003, Irazú's lake underwent a drastic change of color, from clear green to mustard with reddish loops, similar to the color of the waters of Lake Nyos after the gas burst of August 1986. Other studied cold lakes include Botos, Chato, and Tenorio, all at the summit of Quaternary volcanoes as well as Barva and Danta, located in recent pyroclastic cones. Some cold lakes are located in Holocene maar-type explosion craters, among them are Congo, Bosque Alegre, Hule, and Río Cuarto. These last two have undergone repeated rapid reddish color changes over the last 10 years, in association with fish kills and the liberation of apparently sulfurous scents. On March 2010, University of Costa Rica was the host of the 7th Workshop on Volcanic Lakes, part of the Commission of Volcanic Lakes of the IAVCEI, 51 participants from 14 countries attended the workshop; they presented 27 talks and 17 posters, also they visited and sample 4 of the lakes mentioned above (Botos, Irazú, Río Cuarto and Hule). Level of Study: 1: few or no data, 2: regular, 3: acceptable

  8. Avaliação do sistema de produção de semente de haba na serra sul do Peru

    OpenAIRE

    Flores, Mirihan Gamarra

    2010-01-01

    Tendo em conta a importância econômica social da cultura do fava (Vicia faba L.) na serra do Perú, o presente trabalho objetivou avaliar o sistema de produção de sementes de fava pela determinação da qualidade das sementes. Foram utilizadas quatorze lotes de sementes de diferentes classes e categorias, produzidas por INIA e agricultores, nas safras 2006 e 2007, junto com uma amostra de sementes com tegumento manchado e uma amostra de grãos para consumo. Foram utilizadas vários ...

  9. Sigma A recognition sites in the Bacillus subtilis genome

    DEFF Research Database (Denmark)

    Jarmer, Hanne Østergaard; Larsen, Thomas Schou; Krogh, Anders Stærmose

    2001-01-01

    A hidden Markov model of sigma (A) RNA polymerase cofactor recognition sites in Bacillus subtilis, containing either the common or the extended -10 motifs, has been constructed based on experimentally verified sigma (A) recognition sites. This work suggests that more information exists at the ini......A hidden Markov model of sigma (A) RNA polymerase cofactor recognition sites in Bacillus subtilis, containing either the common or the extended -10 motifs, has been constructed based on experimentally verified sigma (A) recognition sites. This work suggests that more information exists...

  10. 白暨豚

    Institute of Scientific and Technical Information of China (English)

    魏卓

    2007-01-01

    世界上有众多的大江大河,分布于其中的淡水鲸类动物仅有4种,它们是南美洲亚马逊河和奥利诺科河水系的亚河豚(Inia geoffrensis)、印度河的印河豚(Platanista minor)、恒河水系中的恒河豚(P.gangetica),以及中国长江中下游的白暨豚(Lipotes vexillifer)。

  11. Diversas dosis de semilla y métodos de siembra en praderas sembradas polifitas

    OpenAIRE

    García Gómez, Armando

    2011-01-01

    Para estudiar las dosis más adecuadas en la siembra de praderas polifitas se efectuaron tinos ensayos en el Centro que el I.N.I.A. posee en Grado (Asturias), observándose que las cantidades utilizadas normalmente por los agricultores pueden ser reducidas considerablemente sin perjuicio para la producción y conservación de aquéllas. Análogamente se hicieron comparaciones entre siembras a máquina y a voleo, con dosis normales y mitad, sin observar diferencias en la calidad y producción de las p...

  12. Estudio de polifenoles, taninos y métodos químicos para determinar la actividad antioxidante de la semilla de Sacha Inchi (Plukenetia volubilis L)

    OpenAIRE

    2013-01-01

    Sacha inchi, es una euphorbiaceae conocida como maní del Inca. Se encuentra distribuida en el Perú, en San Martín, Huánuco, Amazonas, Madre de Dios y Loreto.OBJETIVODeterminar la actividad de los compuestos fenólicos de las semillas de sacha inchi y su rol en el color, conservación, sabor de los alimentos y bebidas.MATERIAL Y MÉTODOEl presente estudio se realizó con semillas de sacha inchi colectadas en Tarapoto por el Instituto Nacional de Investigación y Extensión Agraria (INIA). En el Cent...

  13. Evaluación y caracterización de recursos fitogenéticos del melón, "campaña 2007"

    OpenAIRE

    Martínez Carrillo, Raquel

    2015-01-01

    En el presente trabajo de fin de carrera se enmarca en el proyecto INIA RF2004- 00006-C10 titulado RECOLECCIÓN, MULTIPLICACIÓN Y EVALUACIÓN DE LOS RECURSOS FOTOGENÉTICOS HORTÍCOLAS PARA SU CONSERVACIÓN EN LOS BANCOS DE GERMOPLASMA, cuyo periodo de vigencia fue de 2006 a 2008 (ambos inclusive) continuación de otro anterior al mismo nombre, cuyo periodo de vigencia fue 2003 a 2005, en el marco de la acción estratégica “ Conservación de los recursos genéticos de interés agroali...

  14. Los inicios de la modernización de la investigación agraria

    Directory of Open Access Journals (Sweden)

    Navarro, Luis

    2007-10-01

    Full Text Available The Spanish Government and the International Bank for Reconstruction and Development (BIRF signed a cooperation agreement in 1971 with the objective to upgrade the level of agricultural research by: a restructuring the National Crop Research Institute (INIA to create commodity oriented National Centers, b changing research planning through the creation of National Commodity oriented Programs, and c training of 200 scientists in international high quality laboratories in the priority areas of Research Centers. Total budget of the project was 28,2 million dollars (equivalent today to about 204 million euros, with BIRF supporting 12,7 million dollars as credit. The project successfully accomplished its main objectives and caused an important change in the quality and orientation of agricultural research that had an high impact from scientific and socio economic points of view. This was specially due to the training program, since the other achievements were loosed after the transfer of INIA regional Centers to the Autonomous Communities.El Gobierno de España y el Banco Internacional de Reconstrucción y Fomento (BIRF firmaron en 1971 un Proyecto de colaboración con el objetivo de mejorar el nivel de la investigación agraria mediante tres estrategias fundamentales: a reestructuración del Instituto Nacional de Investigaciones Agrarias (INIA para crear Centros especializados por cultivos con ámbito de actuación nacional, b cambio en la planificación y gestión de la investigación mediante la creación de Programas Nacionales por cultivos y c formación de 200 investigadores en laboratorios internacionales de prestigio en temas prioritarios para las investigaciones a realizar en los Centros. El presupuesto global del Proyecto fue de 28,2 millones de dólares (equivalentes a unos 204 millones de euros actuales, de los que el BIRF aportó 12,7 millones de dólares en forma de crédito. El proyecto cumplió todos los objetivos fundamentales

  15. SEROPREVALENCIA DE LEPTOSPIROSIS EN OVINOS DE DOS GANADERÍAS DE PUNO, PERÚ.

    OpenAIRE

    Bautista R., Lucy; Laboratorio de Medicina Veterinaria Preventiva, Facultad de Medicina Veterinaria, Universidad Nacional Mayor de San Marcos, Lima.; Suárez A., Francisco; Laboratorio de Medicina Veterinaria Preventiva, Facultad de Medicina Veterinaria, Universidad Nacional Mayor de San Marcos, Lima.; Huanca L., Wilfredo; Laboratorio de Reproducción Animal,Facultad de Medicina Veterinaria, Universidad Nacional Mayor de San Marcos, Lima

    2014-01-01

    El objetivo del estudio fue determinar la seroprevalencia de Leptospira spp en ovinos de dos ganaderías del departamento de Puno, Perú. Se recolectaron sueros de 100 ovinos entre marzo y mayo de 2006 de la Estación Experimental ILLPA (INIA) y de la Ganadería Asiruni. Las muestras se procesaron mediante la prueba serológica de aglutinación microscópica. La seroprevalencia total fue de 57%, sin diferencias entre ganaderías.

  16. Biochemistry Identification and Drug Sensitivity Test of Pathogenic Streptococcus from Tilapia in Guangxi%广西罗非鱼链球菌病病原的生化鉴定及药敏试验

    Institute of Scientific and Technical Information of China (English)

    李波; 陈明; 李莉萍; 陈汉忠; 徐增辉; 李超; 黄维义; 梁万文

    2008-01-01

    从来自南宁、北海等地的十几个暴发疾病的罗非鱼养殖场发病罗非鱼中,分离得到了8株链球菌.通过生化鉴定及药敏试验,结果表明,分离到的8株致病性链球菌,有6株为海豚链球菌(streptococcus iniae),2株为无乳链球菌(streptococcus agalactiae).

  17. 常见病害防治方法

    Institute of Scientific and Technical Information of China (English)

    2008-01-01

    @@ 罗非鱼链球菌病 病原:无乳链球菌(Streptococcus agalactiae),海豚链球菌(Streptococcus iniae)也可以引起罗非鱼大量死亡,但危害程度不如无乳链球菌.链球菌是一种革兰氏阳性、非酸性、无运动氧化酶阳性、过氧化氢酶阳性的菌.

  18. Percepţii ale inginerilor silvici asupra vulnerabilităţilor şi riscurilor ecosistemelor forestiere în contextul schimbărilor climatice [Perceptions of forestry engineers on vulnerabilities and risks of forest ecosystems to climate change

    Directory of Open Access Journals (Sweden)

    Mihaela Mutu

    2014-08-01

    Full Text Available A growing recent literature argues that the adaptation to the climate change depends on the perceptions that the stakeholders have on potential effects of the climate change and the possibilities to counteract these effects. This study focuses on forestry engineers’ perceptions on climate changes and adapting measures. A number of 76 semi-structured interviews were conducted with forest engineers from three counties in North-Eastern Romania (Suceava, Neamţ and Botoşani. The results show that the forest engineers perceived as climate change-related vulnerabilities: the occurrence of drought, the risk of increased windfall, and the insects attacks. However, the climate change-related risk is ranked only as a fifth threat on forest ecosystems stability, far behind the political control over forest administration and law implementation, incoherence of the legislative frame, un-appropriate legislation for private forests and illegal logging. We conclude that climate change adaptation is not a priority of forest management, which is correlated with the fact that more than half of the respondents estimates the impact of climate change on forests as being small or moderate, while one third of the respondents clearly manifest the preference for non-adapting behavior.

  19. Caracteristici ale procesului decizional la nivel regional. De la caracteristici la eficienţă administrativă (Features of decizion-making process at the regional level. From features to administrative efficiency

    Directory of Open Access Journals (Sweden)

    Dorina ŢICU

    2014-02-01

    Full Text Available The decision-making process et the administrative level is the mechanism through which the decisions are taken. It is a laborious process that encompasses a multitude of dimensions that overlap the public policies cycle with all its stages (problem definition, choosing alternatives, decision-making process, implementation and evaluation of the decision implemented and that ensures in a less or a greater manner the administrative efficiency, especially in a context of decentralization of this sector or of regionalization. From this point of view, the role of this article is to identify, on the basis of a quantitative study conducted at the level of the administration of the Region of Moldavia, on the city-halls of Botoşani, Iaşi, Bacău, Suceava, Vaslui, Piatra Neamt, Focsani, Galati, the main features of the decision-making process and to place them under an integrated model in order to try to satisfy the principle of efficiency. Outlining such a model has a role in both scientific and comprehensive, but also of good practice-integrator for the various administrative centres in the context of the requirements imposed by the process of regionalization.

  20. A doctoral thesis about Carol Davila written in Paris in 1936.

    Science.gov (United States)

    Rogozea, Liliana; Dumitrascu, Dinu I; Triff, Dorin; Leasu, Florin; Dumitraşcu, Dan L

    2014-01-01

    Carol Davila, the father of the Romanian modern medicine, made decisive contributions to the development of health sciences in the Romanian Principalities in the last decades of the nineteenth century. The merit of his scientific work was recognized beyond the borders of his country. His life (not devoid of anecdotic instances and unknown episodes) and especially his work have aroused considerable interest among numerous medical historians. This paper presents a historical study elaborated in France, but until recently ignored, dedicated to the biography of Carol Davila. It concerns the medical doctoral thesis (State Diploma) elaborated by Joseph Adler (born 1910 in Botoşani, Romania) under the supervision of professor Maxime Laignel-Lavastine, whose interest in Romania is well-known. Professor Laignel-Lavastine held the office of Secretary General of the International Society of History of Medicine (ISHM), founded in 1921, (replaced in this position by another French professor with links to Romania: Jules Guiart). The thesis comprises 48 pages and an exhaustive bibliography. It represents a token of the appreciation given to Davila's achievements by Europe's medical community.

  1. Indexes and efficiencies of N optimum dose reviewed as water- and Nitrogen- footprint

    Science.gov (United States)

    Castellanos, Maria Teresa; Cartagena, Maria Carmen; Cabello, Maria Jesus; Rivas, Francisco; Tarquis, Ana Maria; Arce, Augusto

    2013-04-01

    In order to establish rational nitrogen (N) fertilization and reduce groundwater contamination, a clearer understanding of the N distribution through the growing season and its balance is crucial. In three successive years, a melon crop (Cucumis melo L. cv. Sancho) was grown under field conditions to determine the uptake of N fertilizer, applied by means of fertigation at different stages of plant growth. In addition, Strategies are being sought to increase water use in cropping systems and to reduce drainage. The estimation of N mineralized from soil organic matter is an essential tool to determine the amount necessary to optimize crop yield and minimize the environmental impact of excess N. In this study we propose a methodology that allows us to study fertigated management integrating several aspects: economic and environmental. Even the complexity of the system, we have reduced the number of indexes and efficiencies need to establish the framework of N management and its economical and environmental consequences. At the same time, we have translated all them into a water- and Nitrogen- footprint in each year. ACKNOWLEDGEMENTS This work has been partially supported by INIA under Project INIA-RTA 2010-00110-C03-02

  2. Efficiency and environmental indexes to evaluate the sustainability of mineral and organic fertilization in an irrigated melon crop

    Science.gov (United States)

    Requejo Mariscal, María Isabel; Villena Gordo, Raquel; Cartagena Causapé, María Carmen; Arce Martínez, Augusto; Ribas Elcorobarrutia, Francisco; Jesús Cabello Cabello, María; María Tarquis Alfonso, Ana; Castellanos Serrano, María Teresa

    2014-05-01

    Melon is traditionally cultivated in fertigated farmlands in the center of Spain with high inputs of water and N fertilizer. Excess N can have a negative impact, from the economic point of view, since it can diminish the production and quality of the fruit, from the environmental point of view, since it is a very mobile element in the soil and can contaminate groundwater. From health point of view, nitrate can be accumulated in fruit pulp, and, in addition, groundwater is the fundamental supply source of human populations. Best management practices are particularly necessary in this region as many zones have been declared vulnerable to NO3- pollution (Directive 91/676/CEE) During successive years, a melon crop (Cucumis melo L.) was grown under field conditions applying mineral and organic fertilizers under drip irrigation. Different doses of ammonium nitrate were used as well as compost derived from the wine-distillery industry which is relevant in this area. The present study reviews the most common N efficiency indexes [1] under the different management options with a view to maximizing yield and minimizing N loss. Acknowledgements: This project has been supported by INIA-RTA04-111-C3 and INIA-RTA2010-00110-C03-01. [1] Castellanos, M., Tarquis, A., Ribas, F., Cabello, M., Arce, A., & Cartagena, M. (2013). Nitrogen fertigation: An integrated agronomic and environmental study. Agricultural Water Management, 120, 46-55.

  3. First molecular cloning and gene expression analysis of teleost CD42 (glycoprotein Ib beta chain) GPIb-IX-V subunit from rock bream, Oplegnathus fasciatus.

    Science.gov (United States)

    Jeong, Ji-Min; Kim, Ju-Won; Kim, Do-Hyung; Park, Chan-Il

    2015-04-01

    CD42 is a platelet membrane glycoprotein Ib that plays a key role in haemostasis and thrombin-induced platelet activation. Here, we report the molecular cloning and sequence analysis of the CD42c gene from rock bream (Oplegnathus fasciatus). Rock bream CD42 (RbCD42c) gene expression profiles were determined after infection with Streptococcus iniae, Edwardsiella tarda and red seabream iridovirus (RSIV). The full-length RbCD42c cDNA contained an open reading frame of 624 bp encoding 207 amino acids. The deduced amino acid sequences of the leucine-rich repeat (LRR)-N terminal and LRR-C terminal were conserved between fish and mammals. RbCD42c was highly expressed in red blood cells, spleen, gill, liver and kidney of healthy rock bream. The RbCD42c gene was not significantly up- or downregulated after E. tarda exposure. However, RbCD42c gene expression was upregulated in kidney, spleen and gill after S. iniae infection. RbCD42c was upregulated in spleen, liver and gill, but downregulated in kidney 24 and 48 h after RSIV infection. These results suggest that RbCD42c has different expression patterns after infection with bacterial or viral pathogens. This gene may be directly involved in haemostasis.

  4. Study on Detection of Covert Channel in Flume System%Flume系统的隐蔽信道搜索问题研究

    Institute of Scientific and Technical Information of China (English)

    曹珲; 熊胜超; 张焕国; 严飞

    2013-01-01

    Flume系统不仅可以为处于不同安全级别的进程传输信息提供安全保障,还可以通过显式标签机制解决在隐式标签系统中进程间通信连接超时导致的隐蔽信道问题.但是其系统中的部分不合理标签分配机制可能会导致信息在传递过程中同样存在泄露问题.针对这个问题提出一种隐蔽信道搜索模型(covert channel detection model,CCDM),将隐蔽信道的搜索问题抽象为有向图联通问题.最后结合回溯算法的思想提出IniaPathSearch算法和QuickPathSearch算法来对隐蔽信道进行自动搜索.实验结果表明,IniaPathSearch算法和QuickPathSearch算法可以正确有效地对Flume系统中隐蔽信道进行检测,并能为信息传递提供合法最短路径,其结果可以用于指导提高系统的安全性.

  5. PENAPISAN ISOLAT BAKTERI Streptococcus spp. SEBAGAI KANDIDAT ANTIGEN DALAM PEMBUATAN VAKSIN, SERTA EFIKASINYA UNTUK PENCEGAHAN PENYAKIT STREPTOCOCCOSIS PADA IKAN NILA, Oreochromis niloticus

    Directory of Open Access Journals (Sweden)

    Taukhid Taukhid

    2011-04-01

    Full Text Available Riset dengan tujuan untuk memperoleh isolat kandidat yang imunogenik bagi pembuatan vaksin untuk pengendalian penyakit streptococcosis pada ikan nila telah dilakukan. Karakterisasi dilakukan secara biokimia dan API 20 STREP terhadap 15 isolat bakteri Streptococcus spp. Uji Koch’s Postulate kemudian dilakukan untuk mengetahui peran bakteri pada infeksi streptococcosis pada ikan nila. Konfirmasi taksonomis hingga level spesies isolat bakteri S. agalactiae dilakukan dengan teknik Polymerase Chain Reaction (PCR dengan menggunakan primer spesifik. Uji patogenisitas dilakukan terhadap 6 isolat yang terdiri atas 5 isolat S. agalactiae (N3M, N4M, N14G, N17O, NK1 dan 1 isolat S. iniae (N2O. Hasil penapisan menunjukkan bahwa bakteri S. agalactiae (N4M memiliki nilai LD50 terkecil, dan nilai terbesar dimiliki oleh bakteri S. iniae (N2O. Isolat bakteri N4M digunakan sebagai sumber antigen dalam pembuatan vaksin anti streptococcosis. Vaksin disiapkan dalam bentuk sel utuh dan diinaktivasi dengan formalin, pemanasan, dan sonikasi. Nilai titer antibodi dan sintasan tertinggi diperoleh pada kelompok ikan yang divaksin dengan formalin killed vaccine dibandingkan dengan teknik inaktivasi lainnya (heat killed vaccine dan sonicated vaccine.

  6. A prebiotic role of Ecklonia cava improves the mortality of Edwardsiella tarda-infected zebrafish models via regulating the growth of lactic acid bacteria and pathogen bacteria.

    Science.gov (United States)

    Lee, WonWoo; Oh, Jae Young; Kim, Eun-A; Kang, Nalae; Kim, Kil-Nam; Ahn, Ginnae; Jeon, You-Jin

    2016-07-01

    In this study, the beneficial prebiotic roles of Ecklonia cava (E. cava, EC) were evaluated on the growth of lactic acid bacteria (LAB) and pathogen bacteria and the mortality of pathogen-bacteria infected zebrafish model. The result showed that the original E. cava (EC) led to the highest growth effects on three LABs (Lactobacillus brevis, L. brevis; Lactobacillus pentosus, L. pentosus; Lactobacillus plantarum; L. plantarum) and it was dose-dependent manners. Also, EC, its Celluclast enzymatic (ECC) and 100% ethanol extracts (ECE) showed the anti-bacterial activities on the fish pathogenic bacteria such as (Edwardsiella tarda; E. tarda, Streptococcus iniae; S. iniae, and Vibrio harveyi; V. harveyi). Interestingly, EC induced the higher production of the secondary metabolites from L. plantarum in MRS medium. The secondary metabolites produced by EC significantly inhibited the growth of pathogen bacteria. In further in vivo study, the co-treatment of EC and L. plantarum improved the growth and mortality of E. tarda-infected zebrafish as regulating the expression of inflammatory molecules such as iNOS and COX2. Taken together, our present study suggests that the EC plays an important role as a potential prebiotic and has a protective effect against the infection caused by E. tarda injection in zebrafish. Also, our conclusion from this evidence is that EC can be used and applied as a useful prebiotic.

  7. Transmission and pathology of Streptococcus inane in monosex Nile tilapia (Oreochromis niloticus in aquaculture of Bangladesh

    Directory of Open Access Journals (Sweden)

    Md. Mer Mosharraf Hossain

    2014-04-01

    Full Text Available Streptococcus iniae is a major fish pathogen, recently emergent outbreaks were recorded in commercially cultured monosex Nile tilapia (Oreochromis niloticus result in significant losses termed “streptococcosis”-causes unusual appearances with multi-focal pin-point haemorrhages, abscesses, necrosis and ascites in skin, fin, muscle, liver, spleen, kidney, blood, interstitial fluid specially in central nervous system and brain. This disease was more prevalent (>26% at summer when the water temperature was approximately >25oC, percentage of mortality was higher >41% during the overcrowding and improper water chemistry. Raised levels of glucose and ammonium in blood serum causes reduced number of free blood cells released into the haemolymph to stomach and gut, result in refrain from eating in diseased tilapia. Stocking density (200 fish/decimal; class IV had significant effect (P<0.01 on the total production (5,000 to 5,500 kg/ha. S. iniae in the circulating blood cells, extra-tubular haemal spaces containing blood vessels, fixed phagocytes in the hepatopancreas (gastrointestinal tract, bacteria-like particles in the brain tissue, vacuum and necrosis in hepatocytes revealed with histopathology. In vitro study revealed that cohabitation of dead or infected fish with healthy fish resulted infection (horizontal transmission mechanism to the healthy fish.

  8. The antiquity of riverine adaptations in Iniidae (Cetacea, Odontoceti) documented by a humerus from the late Miocene of the Ituzaingó Formation, Argentina.

    Science.gov (United States)

    Gutstein, Carolina Simon; Cozzuol, Mario Alberto; Pyenson, Nicholas D

    2014-06-01

    "River dolphins" are a paraphyletic group of toothed whales (Odontoceti) that represent independent secondary invasions of freshwater habitats. Different "river dolphin" lineages display suites of convergent morphological specializations that commonly reflect adaptations to riverine and freshwater environments, such as longirostry, reduced orbits, and wide, paddle-like flippers. One lineage, the Iniidae, is presently endemic to South America, and includes several extinct Neogene taxa along with their sole extant genus, Inia (the Amazon River dolphin). We report here a humerus recovered from the late Miocene deposits of the Ituzaingó Formation in the Paraná Basin of Argentina. The specimen exhibits diagnostic features of the family Iniidae, including a scapular-sternal joint of the humerus, which is a unique anatomical connection among mammals. This joint permits enhanced parasagittal adduction of the flipper as a control surface, relative to other odontocetes, providing Inia with a high degree of maneuverability in its structurally complex and heterogenous riverine habitat. This unique anatomical connection, here documented from the late Miocene (∼9 million years-6.5 million years old), not only provides the oldest diagnostic record for Iniidae, but it also indicates a similar habitat use for this lineage, a finding coincident with the current paleoenvironmental interpretation for the Ituzaingó Formation.

  9. Dietary supplementation of cumin (Cuminum cyminum preventing streptococcal disease during first-feeding of Mozambique tilapia (Oreochromis mossambicus

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    Sevdan Yılmaz

    2013-01-01

    Full Text Available This study was conducted to investigate the effect of dietary cumin (Cuminum cyminum powder (CP as a feed additive on growth performance and disease resistance during first-feeding of Mozamique tilapia (Oreochromis mossambicus. Five isonitrogenous (40% crude protein and isocaloric (18.9 kj g-1 diets were formulated to contain 0 (control, 0.5, 1, 1.5, and 2.0% CP. In a 45-day feeding trial, 15 plastic tanks (21 L were stocked with 40 fry (0.012 ± 0.001 g each. After feeding experiment, fish were infected with Streptococcus iniae and mortalities were recorded. The second-order polynomial regression indicated that a dietary CP level of 1.14% provided the best survival rate challenge infection with S. iniae, growth performance and feed utilization. In conclusion, CP can be used as growth promoter to improve feed utilization and weight gain in tilapia fry, and it can be also used as an antimicrobial agent during first-feeding of O. mossambicus. Therefore, CP can be suggested as an alternative to antibiotics in controlling streptococcal disease in tilapia culture.

  10. EFFECT OF NITROGEN-FIXING BACTERIA ON GRAIN YIELD AND DEVELOPMENT OF FLOODED IRRIGATED RICE

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    AMAURI NELSON BEUTLER

    2016-01-01

    Full Text Available This study aimed at evaluating the effect of Azospirillum brasilense , a nitrogen - fixing bacterium, on flooded irrigated rice yield. Evaluations were carried out in a shaded nursery, with seedlings grown on an Alfisol. Were performed two sets of experiments. In the first, were carried out four experiments using the flooded rice cultivars INIA Olimar, Puitá Inta - CL, Br Irga 409 and Irga 424; these trials were set up as completely randomized design in a 5x4 factorial scheme, with four replications. Treatments consisted of five nitrogen rates (0, 40, 80, 120 and 160 kg ha - 1 and four levels of liquid inoculant Ab - V5 and Ab - V6 - A. brasilense (0, 1, 2 and 4 times the manufacturer's recommendation without seed treatment. In second set, were performed two experiments using the cultivars Puitá Inta - CL and Br Irga 409, arranged in the same design, but using a 4x2 factorial. In this set, treatments were composed of four levels of Ab - V5 and Ab - V6 - A. brasilense liquid inoculant (0, 1, 2 and 4 times the recommendation of 100 mL ha - 1 , using rice seeds with and without insecticide and fungicide treatment. Shoot dry matter, number of panicles, and rice grain yield per pot were the assessed variables. The results showed that rice seed inoculation with A. brasilense had no effects on rice grain yield of the cultivars INIA Olimar, Puitá Inta - CL, Br Irga 409 and Irga 424.

  11. Genomic characterization, expression analysis, and antimicrobial function of a glyrichin homologue from rock bream, Oplegnathus fasciatus.

    Science.gov (United States)

    Kasthuri, Saranya Revathy; Wan, Qiang; Umasuthan, Navaneethaiyer; Bathige, S D N K; Lim, Bong-Soo; Jung, Hyung-Bok; Lee, Jehee; Whang, Ilson

    2013-11-01

    Antimicrobial peptides are important innate effector molecules, playing a vital role in antimicrobial immunity in all species. Glyrichin is a transmembrane protein and an antibacterial peptide, exerting its functions against a wide range of pathogenic bacteria. In this study, cDNA and a BAC clone harboring the glyrichin gene were identified from rock bream and characterized. Genomic characterization showed that the OfGlyrichin gene exhibited a 3 exon-2 intron structure. OfGlyrichin is a 79-amino-acid protein with a transmembrane domain at (22)GFMMGFAVGMAAGAMFGTFSCLR(44). Pairwise and multiple sequence alignments showed high identity and conservation with mammalian orthologues. Phylogenetic analysis showed a close relationship with fish species. Higher levels of OfGlyrichin transcripts were detected in the liver from healthy rock bream which were induced by immunogens like lipopolysaccharide, poly I:C, rock bream irido virus, Edwardsiella tarda and Streptococcus iniae. The synthetic peptide (pOf19) showed antibacterial activity against Escherichia coli, E. tarda, and S. iniae. Analysis of the bacterial morphological features after pOf19 peptide treatment showed breakage of the cell membrane, affirming that antibacterial function is accomplished through membrane lysis. The pOf19 peptide also showed antiviral activity against RBIV infection. The high conservation of the genomic structure and protein, together with the antimicrobial roles of OfGlyrichin, provide evidence for the evolutionary existence of this protein playing a vital role in innate immune defense in rock bream.

  12. Mechanical characterization of sawn timber for structural use, graded visually in accordance with Spanish standard UNE 56544

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    Fernández-Golfín, J. I.

    1998-12-01

    Full Text Available The employment of timber in structures in Spain was hurting of the nonexistence of accepted calculation procedures. The recent adoption and publication of the Eurocode 5 compels to the employment of the characteristic values of the material in the structural calculation by the method of the finite elements. This report sets forth the results of the experiments carried out at INIA's Forest Research Centre (CIFOR-INIA regarding the mechanical characterization of sawn timber of size 150x50x3.000 mm and the structural uses of "Pinus sylvestris, P. radiata and P. pinaster" from different Spanish provenances. The characteristic values and strength classes corresponding with the grades stated in the Spanish standard UNE 56544 are also provided. The report compares and analyses the results of these experiments, and presents the conclusions drawn from the different behaviour shown by the four species. An analysis of the Spanish standard for the visual grading of structural timber is carried out on the three species.

    El empleo de la madera en la construcción en España adolecía de la inexistencia de procedimientos de cálculo normalizados. La reciente adopción y publicación del Eurocódigo 5 obliga al empleo de los valores característicos del material en el cálculo estructural de la madera por el método de los elementos finitos. Se presentan los resultados de la caracterización mecánica de la madera aserrada de tamaño (150x50x3.000 mm y usos estructurales de "Pinus sylvestris, P. radiata y P. pinaster" de diferentes procedencias, llevada a cabo en el Centro de Investigación Forestal (CIFOR del INIA. Se aportan los valores característicos y clases de resistencia que corresponden a las clases de calidad definidas en la norma UNE 56544. Se efectúa una comparación y análisis de los resultados obtenidos, extrayendo conclusiones respecto del diferente comportamiento observado para las cuatro especies. Se analiza la adecuación de la

  13. The influence of seasonality, tide and time of activities on the behavior of Sotalia guianensis (Van Bénéden (Cetacea, Delphinidae in Pernambuco, Brazil Influência da sazonalidade, maré e horário de atividades sobre o comportamento de Sotalia guianensis (Van Bénéden (Cetacea, Delphinidae em Pernambuco, Brasil

    Directory of Open Access Journals (Sweden)

    Janaina P. Araújo

    2007-12-01

    écie em ocupar ambientes mais protegidos, sendo o porto do Recife o principal ponto de concentração da espécie. Piedade é apenas uma área exploratória, possivelmente pela ocorrência de tubarões. Olinda tem uso ocasional. As áreas de concentração parecem estar destinadas à alimentação, e a incidência de condutas caudais sugere que a maioria das presas dos botos-cinza é demersal. As condições ambientais (sazonalidade, maré e horário não se mostraram significativas quando relacionadas com as condutas realizadas pelos botos-cinza.

  14. Įdėmus žvilgsnis į paslaptingą gandų fenomeną

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    Juozas Bagdanavičius

    2011-04-01

    Full Text Available Sunku įsivaizduoti žmonių gyvenimą ir bendravimą be gandų. Viena vertus, jie suteikia galimybę sužinoti apie asmenį, įvykį ar reiškinį daugiau ir tuo patenkinti mūsų žmogišką smalsumą. Kita vertus, gauta netikėta žinia, lig tol slėpta, nors ir nepatikrinta informacija praplečia mūsų informacijos šaltinių spektrą, palengvina bendravimą ir sukuria savito pasitikėjimo ir naujo netikėto požiūrio į reiškinį galimybę.Straipsnis lietuvių kalba

  15. STUDIES ON PHYSIOLOGICAL AND BIOCHEMICAL RESPONSES OF ROBINIA PSEUDOA CA CIA CLONES UNDER WATER STRESS%水分胁迫下刺槐不同无性系生理生化反应的研究

    Institute of Scientific and Technical Information of China (English)

    张莉; 续九如

    2003-01-01

    Water stress can cause all kinds of physiological and biochemical responses on forest tree, which are indicated by changes of many physiological and biochemical indexes. Under the different drought extent of soil, the seedlings of Rob-inia pseudoacacia clones were tested. Testing indexes included water content, the ratio of bound water content and free wa-ter content ( Vα/Vs), free proline, soluble sugar, soluble protein ect. The results showed that water content decreased with the increase of soil drought, the rest indexes increased in different extent. The range of variance differed among clones. It was difficult to evaluate exactly the drought resistance of different clones depending on only physiological and biochemical index.

  16. Analysis of Genetic Variability among thirty accessions of Andean Lupin (Lupinus mutabilis Sweet using ISSR molecular markers

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    Michelle C. Chirinos-Arias

    2015-03-01

    Full Text Available In order to make the genetic variability analysis among thirty accessions of andean lupine (L. mutabilis Sweet belonging to Agrarian Innovation National Institute (INIA Seed Bank. DNA was extracted from 300 plants and we made bulks. We standardized amplification protocol of Inter Simple Sequence Repeat (ISSR primers, we chose the most polymorphic primers to run in acrylamide gel. We found 255 ISSR loci with 8 primers. It was found high genetic variability of the samples under study by ISSR markers. Also observed relatively high polymorphism for autogamous species such as andean lupine. Finally phenograms showed a relationship with the geographical location, possibly due to in situ gene flow due to the exchange or sale of seeds in markets near the collection area.

  17. Short Term Impact of the Chilean Journal of Agricultural Research: A Bibliometric Analysis Impacto de Corto Plazo de Chilean Journal of Agricultural Research: Un Análisis Bibliométrico

    Directory of Open Access Journals (Sweden)

    Erwin Krauskopf

    2012-03-01

    Full Text Available In January 2007, the Chilean Journal of Agricultural Research was indexed by the Institute of Scientific Information (ISI. This paper reviews the research that has been published since 2007 by using records extracted from the Web of Science database. The papers published were mostly affiliated to researchers from Chile, and six out of the ten most-contributing countries were from Latin America. The analysis by institutions showed Universidad de Concepcion as the most prolific, although this result is not valid. A lack of standardization in the manner the Instituto de Investigaciones Agropecuarias (INIA subscribed its address on each paper caused a disaggregation of the information. This was proven by the manual curation of each record that was affiliated to any of the centers belonging to INIA. The journal has a self-citation rate of 19.3%, value that is relatively high if compared to other journals from the same subject category listed on The Journal Citation Reports 2010. Finally, this work should be considered a bibliometric snapshot of the current situation of the journal that will serve as a benchmark when new evaluations are made in a few-years time.En enero 2007, la revista Chilean Journal of Agricultural Research fue indexada por el Institute of Scientific Information (ISI, por lo que este artículo analiza la investigación que ha sido publicada desde el año 2007 utilizando los registros extraídos de la base de datos Web of Science. Los artículos publicados fueron mayormente afiliados a investigadores de Chile, siendo seis de los 10 países que más contribuyeron en artículos de Latinoamérica. Un análisis por institución mostró a la Universidad de Concepción como la más prolífica, aunque este resultado no es válido por una ausencia de estandarización en la manera que el Instituto de Investigaciones Agropecuarias (INIA suscribe su dirección en cada artículo, lo que provoca disgregación de la información. Esto se verific

  18. Influence of Conservation Tillage and Soil Water Content on Crop Yield in Dryland Compacted Alfisol of Central Chile Influencia de la Labranza de Conservación y el Contenido de Agua sobre el Rendimiento del Cultivo en un Alfisol compactado del Secano Central de Chile

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    Ingrid Martinez G

    2011-12-01

    Full Text Available Chilean dryland areas of the Mediterranean climate region are characterized by highly degraded and compacted soils, which require the use of conservation tillage systems to mitigate water erosion as well as to improve soil water storage. An oat (Avena sativa L. cv. Supernova-INIA - wheat (Triticum aestivum L. cv. Pandora-INIA crop rotation was established under the following conservation systems: no tillage (Nt, Nt + contour plowing (Nt+Cp, Nt + barrier hedge (Nt+Bh, and Nt + subsoiling (Nt+Sb, compared to conventional tillage (Ct to evaluate their influence on soil water content (SWC in the profile (10 to 110 cm depth, the soil compaction and their interaction with the crop yield. Experimental plots were established in 2007 and lasted 3 yr till 2009 in a compacted Alfisol. At the end of the growing seasons, SWC was reduced by 44 to 51% in conservation tillage systems and 60% in Ct. Soil water content had a significant (p En Chile, las zonas de clima mediterráneo se caracterizan por suelos altamente degradados y compactados por erosión, lo que requiere el uso de sistemas de labranza conservacionista para mitigar la erosión hídrica, así como incrementar el contenido de agua en el suelo. Se evaluó una rotación avena (Avena sativa L. cv. Supernova-INIA - trigo (Triticum aestivum L. cv. Pandora-INIA establecida bajo los siguientes sistemas conservacionistas: cero labranza (Nt, Nt + curvas de nivel (Nt+Cp, Nt + franjas vivas (Nt+Bh y Nt + subsolado (Nt+Sb, las que fueron comparadas al sistema de labranza convencional (Ct, para evaluar su influencia en el contenido de agua en el suelo (SWC en el perfil (10 a 110 cm profundidad, la compactación del suelo y su interacción con el rendimiento del cultivo. Las parcelas experimentales fueron establecidas 3 años seguidos (2007 al 2009 en un Alfisol compactado. Al final de la temporada, el SWC disminuyó 44 a 51% en los sistemas conservacionistas y 60% en el sistema convencional. El sistema de

  19. Airport Activity Statistics of Certificated Route Air Carriers

    Science.gov (United States)

    1987-12-31

    Nin D - 0 0 ’ 0’ 0 C70 ’C7 1 0 > NE’ ( ’ ’ I , M In 0 N N 01a 0 0 0 7 C’ LA11 rCAa w 0’ w’ w C’)jL, : . LL3; . .5 8 CS) (7 (7 NCN U,4 GU 0A 0A 0 5 0 5 L...D InIA a~ N - A~ Ca in Nca R a 0)( 0-0 __NI_ V .)NN& 0L Ca C~C~ rCaa C4 r)--.CI 00 WOA- W IN CNC-C)A IOIIO an - N N MC4aI1f~4C I. 0 C~O 4 C AI~I0-0Na

  20. Bacterial infections from aquatic species: potential for and prevention of contact zoonoses.

    Science.gov (United States)

    Haenen, O L M; Evans, J J; Berthe, F

    2013-08-01

    As aquaculture production and the consumption of aquaculture products increase, the possibility of contracting zoonotic infections from either handling or ingesting these products also increases. The principal pathogens acquired topically from fish or shellfish through spine/pincer puncture or open wounds are Aeromonas hydrophila, Edwardsiella tarda, Mycobacterium marinum, Streptococcus iniae, Vibrio vulnificus and V. damsela. These pathogens, which are all indigenous to the aquatic environment, have also been associated with disease outbreaks in food fish. Outbreaks are often related to management factors, such as the quality and quantity of nutrients in the water and high stocking density, which can increase bacterial loads on the external surface of the fish. As a result, diseased fish are more likely to transmit infection to humans. This review provides an account of human cases of zoonoses throughout the world from the principal zoonotic pathogens of fish and shellfish.

  1. Natūraliųjų vaistingųjų medžiagų, vartotų Alytaus rajono Daugų apylinkėje 2013 metais, etnofarmacinis tyrimas

    OpenAIRE

    Ščerba, Elena

    2014-01-01

    Tyrimo tikslas - surinkti ir susisteminti Alytaus rajono Daugų apylinkėje išlikusias etnofarmacines žinias. Tyrimo uždaviniai: susisteminti surinktą etnofarmacinę medžiagą Alytaus rajono Daugų apylinkėje pagal gydymui vartojamų priemonių prigimtį; nustatyti, kokios kilmės vaistingųjų medžiagų tiriamojoje teritorijoje vartojama daugiausia; nustatyti dažniausiai gydymui vartojamus vaistinius augalus, gyvūnus, grybų rūšis ir mineralinės kilmės medžiagas; nustatyti dažniausias vaistinių augalų va...

  2. Kognityviojo funkcionavimo subjektyvaus ir objektyvaus įvertinimo rodiklių sąsajos

    OpenAIRE

    2014-01-01

    Žmogaus pažintinių funkcijų įvertinimas yra svarbi psichologo (tiek praktiko, tiek teoretiko-mokslininko) veiklos dalis. Nors pasaulyje egzistuoja didelė įvairovė standartizuotų patikimų ir validžių metodikų, kaip žinia, kol kas Lietuvoje turime vos keletą ir tik vaikams skirtų – intelekto vertinimo priemonės WISC-III ir Raveno spalvotos progresuojančios matricos. Suaugusiųjų pažintiniams gebėjimams tirti dažniausiai naudojamos nestandartizuotos priemonės. Tarp jų ypač populiarios atminčiai t...

  3. Production of camu camu plants with different organic substrates in conventional nursery bed

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    Carlos Abanto Rodriguez

    2013-12-01

    Full Text Available The aim was to verify the initial development of camu camu plants with different organic substrates in conventional nursery bedding microsprinkler irrigation and shade management Chromatine® mesh with 50% red light. We used genetic material from mother plants Germplasm Bank of INIA-camu camu Iquitos, seeds were germinated in decomposed sawdust and kept for a period of 40 days after subculturing was performed with 10 cm in height in different substrates according treatments in nursery beds with dimensions of 1.20 m wide x 10 m long with a depth of 30 cm. After conducting assessments of height (cm and basal diameter (mm for a period of 120 days, it was found that the substrate manure has become the substrate for greater efficiency in plant development camu camu substrate followed by humus worm.

  4. Identification of Sand flies of the Subgenus Larroussius based on Molecular and Morphological Characters in North Western Iran

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    A Absavaran

    2009-12-01

    Full Text Available Background: The adult female sand flies (Diptera: Psychodidae of the subgenus Larroussius are important vectors of Leishmania infantum (Kinetoplastida: Tripanosomatidae in Meshkinshahr district, Northwest of Iran. Four Phle­boto­mus (Larroussius species are present in this area, i.e. Phlebotomus (Larroussius kandelakii, P. (La. major, P. (La. perfiliewi and P. (La. tobbi. The objective of the present study was to identify and distinguish the females of P. per­filiewi, P. major and P. tobbi, in this district.Methods: Adult sand flies were collected with sticky papers, CDC light traps, and aspirator in 2006. Individual sand flies of this four species from thirty different locations were characterized morphologically and by comparative DNA se­quences analyses of a fragment of mitochondrial gene Cytochrome b (Cyt b and nuclear gene Elongation Factor 1- al­pha (EF-1α. PCR amplification was carried out for all three species P. major, P. perfiliewi and P. tobbi in the sub­ge­nus Larroussius.Results: Phylogenetic analyses of P. major populations in this study displayed two different populations and genetic di­ver­sity. Spermathecal segment number, pharyngeal armature and other morphological characters of these three spe­cies were examined and found to present consistent interspecific differences.Conclusion: According to our findings, the phylogeny of Cyt b and EF-1α haplotypes confirms the relationships be­tween P. major, P. tobbi and P. perfiliewi as already defined by their morphological similarities.

  5. Occupation pattern of a harbor inlet by the estuarine dolphin, Sotalia guianensis (P. J. Van Bénéden, 1864 (Cetacea, Delphinidae

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    Marta Jussara Cremer

    2009-06-01

    Full Text Available The occupation pattern of Sotalia guianensis in São Francisco do Sul harbor inlet, in Babitonga bay, southern Brazil, was studied between September 1996 and June 1998. A total of 200 h of naturalistic observations and 141.2 h of estuarine dolphin systematic observations were made using binoculars 7 x 50. At each three minutes interval, data about number of individuals and behavior were registered. The population used the harbor inlet intensively, mainly for fishing activities. Ebb tide was responsible for a higher occupation index. Considering the months analyzed, the higher occupation index occurred in May, and in January the lower occurrence was observed. The mean group size was four individuals.Os padrões de ocupação da enseada do porto de cargas de São Francisco do Sul, no interior da Baía da Babitonga, sul do Brasil, pela população de botos cinza Sotalia guianensis foi estudada entre setembro/1996 e junho/1998. Totalizou-se 200 horas de observações naturalísticas e 141.2 horas de observação sistemática na área, utilizando-se binoculares 7 x 50. Foram registrados a cada intervalo de 3 minutos dados sobre o número de indivíduos presentes e comportamento. A população utilizou intensamente a enseada do porto, principalmente para atividades de pesca. A maré enchente foi responsável por maiores taxas de ocupação. Dentre os meses analisados, maio foi o mês com maior média nas taxas de ocupação, e janeiro o mês de menor ocorrência. O tamanho de grupo médio na área foi de quatro indivíduos.

  6. Application of water footprint in a fertirrigated melon crop under semiarid conditions: A review.

    Science.gov (United States)

    Castellanos Serrano, María Teresa; Requejo Mariscal, María Isabel; Villena Gordo, Raquel; Cartagena Causapé, María Carmen; Arce Martínez, Augusto; Ribas Elcorobarrutia, Francisco; Jesús Cabello Cabello, María; María Tarquis Alfonso, Ana

    2015-04-01

    In recent times, there has been a major increase in the use of water and fertilizers in order to increase agricultural production, while at the same time there has increased evidence that aquifers are reducing their water level, enriched by nutrient and degraded as a result of pollution. So best management practices are needed for much of cropped, irrigated and fertirrigated land, to avoid contamination of fresh water and groundwater. The concept of "water footprint" (WF) was introduced as an indicator for the total volume of direct and indirect freshwater used, consumed and/or polluted [1]. The WF distinguishes between blue water (volume of surface and groundwater consumed), green water (rain-water consumed), and grey water (volume of freshwater that is required to assimilate the load of pollutants based on existing ambient water quality standards). This study is focused in calculating the crops WF using a real case of study in a fertirrigated melon crop under semiarid conditions which is principally cultivated in the centre of Spain declared vulnerable zone to nitrate pollution by applying the Directive 91/676/CEE. During successive years, a melon crop (Cucumis melo L.) was grown under field conditions applying mineral and organic fertilizers. Different doses of ammonium nitrate were used as well as compost derived from the wine-distillery industry which is relevant in this area. This application help us to review the different concepts in which is based WF. Acknowledgements: This project has been supported by INIA-RTA04-111-C3 and INIA-RTA2010-00110-C03-01. Keywords: Water footprint, nitrogen, fertirrigation, inorganic fertilizers, organic amendments, winery waste, semiarid conditions. [1] Hoekstra, A.Y. 2003. Virtual water trade. Proceedings of the International Expert Meeting on Virtual Water Trade, Delft, The Netherlands, 12-13 December 2002. Value of Water Research Report Series No. 12, UNESCO-IHE, Delft, The Netherlands.

  7. Effect of mineral and organic fertilization on grey water footprint in a fertirrigated crop under semiarid conditions.

    Science.gov (United States)

    Castellanos Serrano, María Teresa; Requejo Mariscal, María Isabel; Cartagena Causapé, María Carmen; Arce Martínez, Augusto; Ribas Elcorobarrutia, Francisco; Jesús Cabello Cabello, María; María Tarquis Alfonso, Ana

    2016-04-01

    The concept of "water footprint" (WF) was introduced as an indicator for the total volume of direct and indirect freshwater used, consumed and/or polluted [1]. The WF distinguishes between blue water (volume of surface and groundwater consumed), green water (rain-water consumed), and grey water (volume of freshwater that is required to assimilate the load of pollutants based on existing ambient water quality standards). In semiarid scenarios with low water quality, where the irrigation is necessary to maintain production, green WF is zero because the effective rainfall is negligible. As well as blue WF includes: i) extra consumption or irrigation water that the farmer has to apply to compensate the fail of uniformity on discharge of drips, ii) percolation out of control or salts leaching, which depends on the salt tolerance of the crop, soil and quality of irrigation water, to ensure the fruit yield. The major concern is grey WF, because the irrigation and nitrogen dose have to be adjusted to the crop needs in order to minimize nitrate pollution. This study is focused in assessment mineral and organic fertilization on grey WF in a fertirrigated melon crop under semiarid conditions, which is principally cultivated in the centre of Spain declared vulnerable zone to nitrate pollution by applying the Directive 91/676/CEE. During successive years, a melon crop (Cucumis melo L.) was grown under field conditions. Different doses of ammonium nitrate were used as well as compost derived from the wine-distillery industry which is relevant in this area. Acknowledgements: This project has been supported by INIA-RTA04-111-C3 and INIA-RTA2010-00110-C03. Keywords: Water footprint, nitrogen, fertirrigation, inorganic fertilizers, organic amendments, semiarid conditions. [1] Hoekstra, A.Y. 2003. Virtual water trade. Proceedings of the International Expert Meeting on Virtual Water Trade, Delft, The Netherlands, 12-13 December 2002. Value of Water Research Report Series No. 12

  8. Effects of dietary Spirulina platensis on growth performance, humoral and mucosal immune responses and disease resistance in juvenile great sturgeon (Huso huso Linnaeus, 1754).

    Science.gov (United States)

    Adel, Milad; Yeganeh, Sakineh; Dadar, Maryam; Sakai, Masahiro; Dawood, Mahmoud A O

    2016-09-01

    Dietary supplementation of Spirulina platensis at different levels (0% control, 2.5%, 5% and 10%) was evaluated to find out the effects on growth performance, digestive enzyme activities, humoral and skin innate immune responses and disease resistance in the great sturgeon (Huso huso). After 8 weeks of experimental trial, growth parameters, intestinal lactic acid bacteria count, protease and lipase activities were significantly high in 10% S. platensis fed group (P < 0.05). Similarly, in this group, respiratory burst activity of leucocytes and total protein of serum were also significantly high. Furthermore, supplementation of S. platensis at 5 or 10% exhibited higher serum IgM and lysozyme activity than the other experimental groups (P < 0.05). On the contrary, serum triglycerides and number of blood lymphocytes were lower in experimental groups than that of control group. Total proteins, lysozyme, protease and esterase, as well as in vitro bactericidal activity (against Streptococcus iniae, Yersinia ruckeri, Aeromonas hydrophila and Lactococcus garviea) were significantly high in skin mucus from fish fed 5% and 10% S. platensis, while, alkaline phosphatase was significantly high in fish fed 10% S. platensis (P < 0.05). Further, fish infected with Streptococcus iniae bacteria increased mortality, but it was alleviated by a diet supplemented with S. platensis. The present results demonstrate that this dietary supplementation with S. platensis (mainly at 10% level) could be useful for maintaining the overall health status of great sturgeon.

  9. Avances de investigación para la propagación vegetativa por cultivo in vitro de Myrciaria dubia (H.B.K. Mc Vaugh “camu camu”

    Directory of Open Access Journals (Sweden)

    Sergio Pinedo Freyre

    2013-06-01

    Full Text Available El trabajo fue realizado en el Laboratorio de Cultivo de Tejidos Vegetales de la Estación Experimental Agraria San Roque, del INIA Loreto con el objetivo de establecer un protocolo para el cultivo in vitro de segmentos nodales de camu camu" Myrciaria dubia " (H.B.K Mc Vaugh. La fuente de material vegetal, fueron ramas de la parte apical de plantas adultas de la Colección Nacional de camu camu del INIA Loreto, se realizaron ensayos de desinfección, medios de cultivo y diferentes concentraciones de hormonas. El medio de cultivo utilizado fue Murashige & Skoog, MS 1962; más factores constantes 20 g/L sacarosa, 0,25 g/L carbón activado, 7 g/L agar y pH 5,7. Los tratamientos de desinfección que presentaron los mayores porcentajes (100% de supervivencia de segmentos nodales, fueron: Hipoclorito 0,3% + Chupadera 1 g/L en M&S, M&S ½ y M&S + AIB y Hipoclorito 0,5% + Chupadera 2 g/L en M&S, M&S ½ y M&S + AIB. El mejor medio de cultivo para la fase de crecimiento in vitro fue M&S + 1,5 mg/L GA3 + 10 mg/L AIA con 72% de supervivencia a los 45 días después de la siembra. El mejor medio de cultivo para la fase de multiplicación fue M&S + 2,5 mg/L BAP + 0,1 mg/L ANA con 80% de supervivencia a los 30 días después del subcultivo.

  10. EFEKTIVITAS ANTIBIOTIKA DAN VAKSIN DALAM PENANGGULANGAN PENYAKIT STREPTOCOCCOSIS PADA IKAN NILA (Oreochromis niloticus

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    Hambali Supriyadi

    2016-11-01

    Full Text Available Penelitian dengan tujuan untuk mengetahui antibiotik yang efektif untuk pengobatan penyakit streptococcosis, serta mendapatkan cara pencegahan penyakit secara biologis yaitu melalui penggunaan vaksin telah dilakukan di Laboratorium Riset kesehatan Ikan Pasar Minggu. Tiga jenis antibiotika yaitu Neomycin, Oxytetracyclin, dan Enrofloxacin diuji efektivitasnya terhadap 4 isolat bakteri Streptococcus iniae yaitu Y2N7, Y2N9, GM2.4, dan S1N8 melalui uji zona hambatan dan konsentrasi hambat minimum (MIC. Uji imunogenitas diuji dengan cara pembuatan vaksin dari isolat yang digunakan yang kemudian dievaluasi level titer antibodi yang diproduksi. Hasil penelitian menunjukkan bahwa enrofloxacin merupakan antibiotik yang efektif terhadap semua isolat yang diuji, sedangkan neomycin efektif hanya untuk isolat Y2N7. Isolat GM2.4 relatif memiliki sifat immunogenitas lebih baik dibanding dengan isolat uji lainnya. Research with the aims to evaluate the effectiveness of several antibiotics against 4 (four streptococcus iniae isolates, and evaluation of immunogecity of those isolate to be used for disease control (vaccine have been conducted at Fish Health Research Laboratory Pasar Minggu. The effectiveness of three antibiotics namely Neomycin, Oxytetracyclin, and Enrofloxacin have been tested against 4 (four isolates Y2N7, Y2N9, GM2.4, and S1N8. The immunogenicity of those isolates were also tested by developing vaccine and evaluated through the production of antibody titer level. The results indicated that enrofloxacin was effective against all isolates tested, meanwhile neomycin only effective against isolate Y2N7. Isolate of GM2.4 was relatively immunogenic as compared to the other isolates.

  11. Avances de investigación para la propagación vegetativa por cultivo in vitro de Myrciaria dubia (H.B.K. Mc Vaugh “camu camu”

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    Sergio Pinedo Freyre

    2013-06-01

    Full Text Available El trabajo fue realizado en el Laboratorio de Cultivo de Tejidos Vegetales de la Estación Experimental Agraria San Roque, del INIA Loreto con el objetivo de establecer un protocolo para el cultivo in vitro de segmentos nodales de camu camu" Myrciaria dubia " (H.B.K Mc Vaugh. La fuente de material vegetal, fueron ramas de la parte apical de plantas adultas de la Colección Nacional de camu camu del INIA Loreto, se realizaron ensayos de desinfección, medios de cultivo y diferentes concentraciones de hormonas. El medio de cultivo utilizado fue Murashige & Skoog, MS 1962; más factores constantes 20 g/L sacarosa, 0,25 g/L carbón activado, 7 g/L agar y pH 5,7. Los tratamientos de desinfección que presentaron los mayores porcentajes (100% de supervivencia de segmentos nodales, fueron: Hipoclorito 0.3%  + Chupadera 1 g/L en M&S, M&S ½ y M&S + AIB y Hipoclorito 0,5%  + Chupadera 2 g/L en M&S, M&S ½ y M&S + AIB. El mejor medio de cultivo para la fase de crecimiento in vitro fue M&S + 1,5 mg/L GA3 + 10 mg/L AIA con 72% de supervivencia a los 45 días después de la siembra. El mejor medio de cultivo para la fase de multiplicación fue M&S + 2,5 mg/L BAP + 0,1 mg/L ANA con 80% de supervivencia a los 30 días después del subcultivo.

  12. Identification and expression profiles of multiple genes in Nile tilapia in response to bacterial infections.

    Science.gov (United States)

    Pridgeon, Julia W; Aksoy, Mediha; Klesius, Phillip H; Li, Yuehong; Mu, Xingjiang; Srivastava, Kunwar; Reddy, Gopal

    2011-11-15

    To understand the molecular mechanisms involved in response of Nile tilapia (Oreochromis niloticus) to bacterial infection, suppression subtractive cDNA hybridization technique was used to identify upregulated genes in the posterior kidney of Nile tilapia at 6h post infection with Aeromonas hydrophila. A total of 31 unique expressed sequence tags (ESTs) were identified from 192 clones of the subtractive cDNA library. Quantitative PCR revealed that nine of the 31 ESTs were significantly (ptilapia at 6h post infection with A. hydrophila at an injection dose of 10(5)CFU per fish (≈ 20% mortality). Of the nine upregulated genes, four were also significantly (ptilapia at 6h post infection with A. hydrophila at an injection dose of 10(6)CFU per fish (≈ 60% mortality). Of the four genes induced by A. hydrophila at both injection doses, three were also significantly (ptilapia at 6h post infection with Streptococcus iniae at doses of 10(6) and at 10(5)CFU per fish (≈ 70% and ≈ 30% mortality, respectively). The three genes induced by both bacteria included EST 2A05 (similar to adenylate kinase domain containing protein 1), EST 2G11 (unknown protein, shared similarity with Salmo salar IgH locus B genomic sequence with e value of 0.02), and EST 2H04 (unknown protein). Significant upregulation of these genes in Nile tilapia following bacterial infections suggested that they might play important roles in host response to infections of A. hydrophila and S. iniae.

  13. Seed softening patterns of forage legumes in a temperate/subtropical environment in Uruguay

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    Javier Do Canto

    2013-03-01

    Full Text Available Few studies have been conducted in annual and perennial forage legumes to investigate the development of hardseededness and the subsequent pattern of seed softening in temperate and subtropical regions of South America. Experiments were conducted during 2007 and 2008 in central Uruguay to follow the pattern of seed softening in 35 annual and perennial forage legumes, including three native species of Uruguay and five commercial cultivars. Newly ripened seeds of each plant material were placed in mesh packets on the soil surface in mid-summer. Samples were recovered monthly for germination tests and the proportion of residual hard seeds determined. The native species Adesmia bicolor (Poir. DC., Adesmia securigerifolia Herter, and Ornithopus micranthus (Benth. Arechav., together with Ornithopus pinnatus (Mill. Druce cv. INIA Molles behaved similarly. They showed high levels of initial hard seed from 78% in A. bicolor to 99% in A. securigerifolia and O. pinnatus cv. INIA Molles in 2007; displayed pulses of seed softening, particularly in autumn, and retained moderate levels of residual hard seed for the development of a soil seed bank ranging from 15% in A. bicolor to 49% in O. micranthus. These appear to be desirable characteristics for persistence of forage legumes in subtropical grasslands, both for annual and perennial species. Trifolium repens L. and Lotus corniculatus L. produced few hard seeds, only 2% and 13% respectively were hard after 1-mo in the field and were completely soft by July placing extra reliance on their vegetative propagation for persistence. Materials of L. arenarius Brot. showed pronounced late autumn softening, while materials of L. ornithopodioides L. showed extremely high levels of hardseededness (between 96% and 100% and no softening during the evaluation period, apart from two materials that were completely soft seeded. Mediterranean forage legumes should be properly evaluated in temperate and subtropical regions as

  14. Doses e épocas de aplicação de nitrogênio na suscetibilidade do arroz à temperatura baixa na fase reprodutiva Nitrogen doses and timing on rice susceptibility to low temperature in reproductive stage

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    Ramón Méndez Larrosa

    2009-07-01

    Full Text Available A aplicação de nitrogênio (N deve afetar a sensibilidade das plantas de arroz quando submetidas ao frio na fase reprodutiva. Assim, foram instalados dois experimentos em casa de vegetação, no Instituto Nacional de Investigação Agropecuária (INIA, INIA Treinta y Tres, Uruguai, na safra agrícola 2007/08. O primeiro experimento teve o objetivo de determinar o efeito da época e o segundo, o efeito de dose de aplicação de N sobre a suscetibilidade de plantas de arroz irrigado à temperatura baixa na fase reprodutiva. Realizou-se um experimento bifatorial em delineamento inteiramente casualizado (fator A: épocas ou doses de aplicação de N e fator B: aplicação ou não de frio nos dois experimentos, com quatro repetições. No experimento 1, os tratamentos do fator A foram a aplicação de N (30kg ha-1 nos estádios V4, V5, V6, V7, V8, R0, R1+4 dias e a testemunha sem N em cobertura. Nesse experimento, os tratamentos que receberam N de V4 a V8 também receberam mais 30kg ha-1 no estádio R0, e os tratamentos que receberam N nos estádios R0 e R1+4 dias também receberam 30kg ha-1 no estádio V4. No experimento 2, o fator A foram níveis de N de 0, 23, 46 e 69kg ha-1 de N no estádio V4 mais uma aplicação de 23kg ha-1 no estádio R0 e as mesmas doses em R0 com 23kg ha-1 no estádio V4, mais uma testemunha sem aplicação de N nos dois estádios. No tratamento com frio, as plantas foram submetidas a 12,5°C durante sete horas em quatro noites quando o colar da folha bandeira ficou com a mesma altura que o colar da folha anterior. Foram avaliados os seguintes parâmetros: a percentagem de esterilidade de espiguetas, o índice SPAD, realizado aos três, 10 e 16 dias após o tratamento do frio no estádio R2-R3, a produtividade de grãos por vaso, o número de panículas por balde e a massa de mil grãos. A esterilidade de espiguetas não foi influenciada pela época, pela dose de aplicação de N ou pelo tratamento de frio, mas encontrou

  15. Identification of Sand flies of the Subgenus Larroussius based on Molecular and Morphological Characters in North Western Iran

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    A Absavaran

    2009-12-01

    Full Text Available Background: The adult female sand flies (Diptera: Psychodidae of the subgenus Larroussius are important vectors of Leishmania infantum (Kinetoplastida: Tripanosomatidae in Meshkinshahr district, Northwest of Iran. Four Phle­boto­mus (Larroussius species are present in this area, i.e. Phlebotomus (Larroussius kandelakii, P. (La. major, P. (La. perfiliewi and P. (La. tobbi. The objective of the present study was to identify and distinguish the females of P. per­filiewi, P. major and P. tobbi, in this district."nMethods: Adult sand flies were collected with sticky papers, CDC light traps, and aspirator in 2006. Individual sand flies of this four species from thirty different locations were characterized morphologically and by comparative DNA se­quences analyses of a fragment of mitochondrial gene Cytochrome b (Cyt b and nuclear gene Elongation Factor 1- al­pha (EF-1α. PCR amplification was carried out for all three species P. major, P. perfiliewi and P. tobbi in the sub­ge­nus Larroussius."nResults: Phylogenetic analyses of P. major populations in this study displayed two different populations and genetic di­ver­sity. Spermathecal segment number, pharyngeal armature and other morphological characters of these three spe­cies were examined and found to present consistent interspecific differences."nConclusion: According to our findings, the phylogeny of Cyt b and EF-1α haplotypes confirms the relationships be­tween P. major, P. tobbi and P. perfiliewi as already defined by their morphological similarities.                                                                                  Keywords: Phlebotomus, Larroussius, Cytochrome b, Elongation Factor-1α, Morphology, Iran                 

  16. Site fidelity of Sotalia guianensis (Cetacea: Delphinidae in Sepetiba Bay, Rio de Janeiro, Brazil Fidelidade de área de Sotalia guianensis (Cetacea: Delphinidae na Baía de Sepetiba, Rio de Janeiro, Brasil

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    Mariana F. Nery

    2008-06-01

    Full Text Available Estuarine dolphins, Sotalia guianensis (Van Bénéden, 1864, were identified in Sepetiba Bay (22º35'S, 44º03'W, state of Rio de Janeiro, Brazil, between February 1994 and July 2006 using the photo-identification method. A total of 72 surveys resulted in the identification and cataloguing of 217 individual dolphins. Seventy-nine (36% were resighted at least once during the study period. The average interval between resightings was 40,6 ± 31,1 months. Large numbers of mother-and-calf pairs were recorded during the 12 years and new individuals were frequently encountered in the photographic records. These results suggest that part of the dolphin population is more consistently found in Sepetiba Bay, probably for feeding and reproduction, while other individuals use the Bay only sporadically.Indivíduos da população dos golfinhos estuarinos, Sotalia guianensis (Van Bénéden, 1864, da Baía de Sepetiba (22º35'S, 44º03'W Estado do Rio de Janeiro, sudeste do Brasil, foram catalogados pelo método de foto-identificação durante o período de fevereiro de 1994 a julho de 2006. Foram conduzidas 72 expedições de barco, resultando na identificação e catalogação de 217 botos. Destes 79 (36,4% foram recapturados pelo menos uma vez durante o período de estudo; o intervalo médio entre as recapturas foi de 40,6 ± 31,1 meses. A presença de pares de mães-filhotes foi numerosa ao longo dos 12 anos de estudo e novos indivíduos foram constantemente identificados na área. Os resultados sugerem que parte desta população depende substancialmente do habitat fornecido pela Baía de Sepetiba, tanto para alimentação quanto para fins de reprodução, apesar de haver indivíduos que pouco se utilizam desta área.

  17. SEQUENCE ANALYSIS OF Streptococcus agalactiae, A PATHOGEN CAUSING STREPTOCOCCOSIS IN TILAPIA (Oreochromis niloticus

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    Angela Mariana Lusiastuti

    2009-12-01

    Full Text Available Pathogen identification based on biochemical properties can barely differentiate Streptococcus iniae and S. agalactiae. Beside that, this technique is also limited by the length of time required to complete the assays. Therefore, rapid diagnosis is necessary to initiate prompt therapeutic and prophylactic measures in order to limit any potential economic losses caused by such pathogens. The aim of the present study was to identify Streptococcosis species using amplification of S. agalactiae DNA sequence with species-specific primer Sdi 61 AGGAAACCTGCCATTTGCG and Sdi 252 CAATCTATTTCTAGATCGTGG and perform phylogenetic analysis based on DNA nucleotide sequence data. The sequencing of PCR products was performed at BPPT Puspiptek Serpong by using the respective PCR primers, Big Dye Terminator Chemistry and AmpliTaq-FS DNA polymerase. The sequencing reactions were run on the ABI Prism version 3103 – Avant Genetic Analyzer (USA and the result was read by Sequence Navigator program (Applied Biosystem. Alignment multiple analysis was done based on the data from Genebank with BLASTN (http://blast.ncbi.nlm.nih.gov/blast.cgi on the nucleotide level. Neighbor-joining phylogenetic trees were generated with Genetyx programme version 7 with UPGMA and MEGA software version 4.0. The result revealed that the isolates from brain, eye, and kidney of diseased Tilapia were infected by S. agalactiae and it has 99% similarity with Genebank. It has close relationship with S. agalactiae at genebank with UPGMA method. These isolates showed high variation in the first sequence which is similar to S. iniae. The information of S. agalactiae genomes suggests that gene acquisition, duplication, and reassortment have played an important role in genetic diversity and evolution of S. agalactiae. Screening of breeder fish stocks with the developed PCR methodology, followed by elimination of infected stocks, would provide an efficient strategy to control fish

  18. Efeito de diferentes concentrações de sais em solução nutritiva na tolerância de cultivares de trigo à toxicidade de alumínio Influence of salt concentrations in nutrient solution on tolerance to aluminum toxicity in wheat cultivars

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    Carlos Eduardo de Oliveira Camargo

    1981-01-01

    Full Text Available Foram estudados dez cultivares de trigo em soluções nutritivas contendo três diferentes níveis de alumínio tóxico combinados com quatro diferentes concentrações salinas. A tolerância foi medida pela capacidade de as raízes primárias continuarem a crescer em solução sem alumínio, após permanência de 46 horas em solução contendo determinadas concentrações de sais e de alumínio. Os cultivares Siete Cerros, Tobari-66 e INIA-66 foram os mais sensíveis, IAC-15 moderadamente sensível, Alondra S-46 e IAC-17 moderadamente tolerantes, BH-1146, IAC-13, IAC-5 e C-3 tolerantes à presença de quantidades crescentes de Al³+ na solução nutritiva tratamento, o sintoma de toxicidade de alumínio ficou acentuado ou por aumento da concentração de alumínio ou por diminuição da concentração de sais para todos os cultivares estudados. Aparentemente, tolerância à toxicidade de determinada concentração de alumínio é uma característica antes relativa do que absoluta, por depender da concentração de sais presentes.The aluminum tolerance of ten wheat cultivars was studied in nutrient solutions using three different levels of this element combined with four different salt concentrations. The tolerance was evaluated by measuring, the root growth in an aluminum-free complete nutrient solution after a previous treatment in Al added solutions of equal, one half, one fifth and one tenth the salt concentration of the aluminum-free solution. The wheat cultivars Siete Cerros, Tobari-66 and INIA-66 were sensitive; IAC-15 was moderately sensitive; Alondra-S-46 and IAC-17 showed moderate tolerance; BH-1146, IAC-13, IAC-5, and C-3 were tolerant to the presence of increasing concentrations of Al³+ in the treatment nutrient solution. The aluminum toxicity symptom (inhibition of root growth was dependent on the salt concentration and the amount of aluminum in the treatment solution. For the same level of Al3+ toxicity increased when the

  19. 降低交叉反应的鱼类病原菌检测抗体芯片初步研究%PRELIMINARY STUDY ON REDUCTION OF CROSS-REACTION OF ANTIBODY MICROARRAY FOR DETECTION OF PATHOGENIC BACTERIA IN FISH

    Institute of Scientific and Technical Information of China (English)

    王欣欣; 绳秀珍; 战文斌

    2013-01-01

    采用菌体吸附法对海豚链球菌(Streptococcus iniae)、迟缓爱德华氏菌(Edwardsiella tarda)、鳗弧菌(Vibrio anguillarum)、杀鲑气单胞菌(Aeromonas salmonicida)、荧光假单胞菌(Psedomonas fluorescens)和海洋分支杆菌(Mycobacterium marinum)6种养殖鱼类病原菌的兔抗血清进行吸附,以减小抗血清与其它菌株的交叉反应;吸附纯化后的抗体作为捕获抗体构建了病原菌检测抗体芯片.实验结果显示,与未经过吸附的纯化抗血清构建的抗体芯片相比,血清吸附实验能有效减少交叉反应,降低抗体的非特异性吸附,提高细菌检测的特异性.同时,以迟缓爱德华氏菌为例,采用吸附纯化后抗体制备的抗体芯片检测人工感染迟缓爱德华氏菌和自然发病的牙鲆,均观察到迟缓爱德华民菌的特异性显色反应,得到了较理想的检测结果.本文结果说明优化后的病原菌检测抗体芯片可用于水产养殖鱼类常见的上述6种细菌性疾病的有效检测.%Six kinds of bacterias from marine cultured fish, including Streptococcus iniae , Edwardsiella tarda, Vibrio anguillarum , Aeromonas salmonicida , Psedomonas fluorescens and Mycobacterium ma-rinum , were chosen, and their rabbit antiserum were adsorbed to reduce cross reaction by serum adsorption experiment. And then antibody microarrays were developed using the purified rabbit antiserum as capture antibodies. The results showed that the cross reaction of the antibody microarray was reduced effectively, and the specificity and sensibility of bacteria detection were improved greatly after serum adsorption. Meanwhile, taking E. tarda for example, the antibody microarray was applied to detect E. tarda in Paralichthys olivaceus infected artificially and naturally with E. tarda, and specific positive staining were observed, suggesting the developed antibody microarrays could effectively detect the six kinds of bacteria above-mentioned in fish.

  20. Characterization of virulent Listeria monocytogenes isolates recovered from ready-to-eat meat products and consumers in Cairo, Egypt

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    Maysa A. I. Awadallah

    2014-10-01

    Full Text Available Aim: This study aimed to investigate the occurrence of some virulence genes distributed in Listeria monocytogenes isolated from ready-to-eat (RTE meat products and consumers in Cairo province, Egypt. Materials and Methods: A total of 120 beef luncheon, chicken luncheon and frankfurter beef (40 samples, each were collected from 10 different local shops situated in Al-salam city, Cairo province, Egypt. Stool samples were collected from 40 people who had the habit of consuming RTE meat. The suspected L. monocytogenes isolates were subjected to a multiplex polymerase chain reaction (PCR for rapid speciation and virulence determination using primers specific for inIA, inIC, and inIJ genes. Results: Culture examination of all samples on Oxford media revealed presence of colonies characteristic to L. monocytogenes in 6 beef luncheon (15%, 4 chicken luncheon (10%, 1 frankfurter beef (2.5% and 1 human stool (2.5% samples. Species identity of L. monocytogenes was verified through the amplification of a 800 bp fragment with inIA primers in 2 out of 6 culture isolates from beef luncheon (5%, and 1 out 4 culture isolates from chicken luncheon (2.5% samples. Statistical analysis revealed no significant difference between the occurrence of L. monocytogenes in different food samples examined (p>0.05. The virulence of these strains was ascertained by the presence of 517 bp and 238 bp fragments of inIC and inIJ genes, respectively in the isolates that contained the 800 bp fragment. The culture isolates obtained from one frankfurter beef sample, and one human stool sample were found negative by multiplex PCR for the presence of L. monocytogenes and its virulence specific genes. Conclusion: It could be concluded that L. monocytogenes are circulating in beef and chicken luncheon sold in Cairo, Egypt. Multiplex PCR is reliable for confirmation of L. monocytogenes. This study suggests the implementation of hygienic measures at all levels from production to consumption

  1. THE NATIONAL STRUCTURE OF THE ROMANIAN POPULATION AT THE 20TH OF OCTOBER 2011 CENSUS

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    GR. P. POP

    2014-06-01

    Full Text Available The National Structure of the Romanian Population at the 20th of October 2011 Census. This is the third study regarding the national structure of the population of Romania. The first two approached the 1992 and 2002 censuses and were published in Studia UBB, Geographia, 2 (1991 and the Romanian Review of Political Geography (20042. On the census day, Romania had 20,121,641 inhabitants, of which 83.45% (16,792,868 are Romanians, while the other 16.55% belong to: Hungarians 6.10% (1,227,623 inhabitants, Gypsies 3.09% (621,573 people, other 18 national minorities (Ukrainians, Germans, Turks, Russian-Lipovans, Tartars, Serbs, Slovaks, Bulgarians, Croats, Greek, Italians, Jews, Czechs, Poles, Chinese, Armenians, Csangos, Macedonians and the category “other national groups” have together only 1.21% (242,767 inhabitants. There is also a category of people for which the information was ”unavailable”, totaling 6.15% (1,236,810 people of the Romanian population (fig. 1. As requested to assess the essential issues of such a subject, one needs to analyze the distribution of the national minorities across Romania, at the level of the counties and the eight geographical-historical provinces of Romania: Transylvania, Moldavia, Dobrudja, Muntenia, Oltenia, Banat, Crişana and Maramureş. Generally, regarding the presence of the Romanian population, one notices the following situation at the level of the counties: in 18 counties of the total of 41 in Romania, the weight of the Romanian population is over 90%, including all the eight Moldavian counties (Bacău, Botoşani, Galaţi, Iaşi, Neamţ, Suceava, Vaslui, Vrancea, six out of the ten counties of Muntenia (Argeş, Brăila, Buzău, Dâmboviţa, Prahova, Teleorman and four out of five counties of Oltenia (Dolj, Gorj, Olt, Vâlcea. The highest weight of Romanians is recorded in the counties of Gorj (94.17% and Botoşani (94.08%. A weight between 80 and 90% was recorded in five out of the ten counties of

  2. Abstracts of the 41th Symposium on Cuniculture, ASESCU Hondarribia, Spain, 12th-13th May, 2016

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    Abstracts, Conferences, Congresses, Symposiums...

    2016-09-01

    Full Text Available The 41st Congress of the Spanish Association of Cuniculture (ASESCU was held in Hondarribia (Guipúzcoa province from 12th to 13th May 2016. This edition coincided with the 40th anniversary of ASESCU, which was founded in 1976. The main papers focussed on analysing the changes in rabbit feeding in the last decades and future prospects, on preparing the future reproductive rabbit, on Spanish consumer segmentation and characterization of rabbit meat consumption, and on the role of the Rabbit Meat Marketing Board (INTERCUN in promoting consumption of rabbit meat in Spain. A specific session was devoted to explain the objectives and progress of the research projects supported by the partnership between the Spanish Institute of Agricultural Research (INIA and INTERCUN. In addition, a round table was held on the present and future prospects of the rabbit farming in Spain. Moreover, a total of 24 communications were presented both in working sessions with oral communications and posters (nutrition, carcass and meat quality, pathology, ethology and welfare, and management and genetics. The meeting was attended by more than 185 participants, including researchers from Spain, Portugal, Italy, France, Brazil, Egypt, and Puerto Rico among other countries. Abstracts of the contributions presented are reported below.

  3. BIOFILMS BASED ON CANIHUA FLOUR (Chenopodium Pallidicaule: DESIGN AND CHARACTERIZATION

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    Lady M. Salas-Valero

    2015-01-01

    Full Text Available This work aims to (1 produce and characterize the flour obtained from two varieties of canihua, cupi and illpa-inia, and (2 evaluate the ability of these flours to form biofilms. The flours produced contain proteins, starches, lipids, organic substances containing phenol groups, and high percentages of unsaturated fatty acids. Films produced from the illpa variety presented lower water vapor permeability and larger Young’s modulus values than the films formed from the cupi variety. Both films were yellowish and displayed a high light blocking ability (as compared with polyethylene films, which can be attributed to the presence of phenolic compounds. Furthermore, they showed lesser solubility and water permeability than other polysaccharide films, which may be the result of the higher protein (12%–13.8% and lipid (11% contents in canihua flours, as well as the formation of a larger number of S–S bonds. On the other hand, these films presented a single vitreous transition temperature at low temperatures (< 0 °C, crystallization of the A and Vh types, and an additional diffraction peak at 2 = 7.5º, ascribed to the presence of essential fatty acids in canihua flour. Canihua flour can form films with adequate properties and shows promise for potential applications in food packaging, because it acts as a good barrier to incident ultraviolet light.

  4. Análisis de la Variabilidad Genética entre treinta accesiones de tarwi (Lupinus mutabilis Sweet usando marcadores moleculares ISSR

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    Michelle C. Chirinos-Arias

    2015-01-01

    Full Text Available Con el fin de realizar el análisis de variabilidad genética inter-accesión de treinta accesiones de tarwi (L. mutabilis Sweet pertenecientes al Banco de Germoplasma del Instituto Nacional de Innovación Agraria (INIA. Se extrajo el ADN de 300 plantas, se construyeron bulks, se estandarizó el protocolo de amplificación de los marcadores moleculares Inter Simple Sequence Repeat (ISSR, de los cuales se eligió a los más polimórficos y nítidos para corrida en gel de acrilamida. Encontrándose 255 bandas con 8 iniciadores ISSR. El análisis de la variabilidad genética con estos iniciadores comprobó una alta variabilidad genética de las muestras en estudio. Observándose también un polimorfismo relativamente alto para una especie autógama como L. mutabilis. Finalmente los fenogramas mostraron una relación con la ubicación geográfica, posiblemente debido al flujo génico in situ debido al intercambio o venta de semillas en ferias o mercados aledaños a la zona de colecta.

  5. Assessment of different doses of N applied to a melon crop in the quality of groundwater: environmental impact indexes.

    Science.gov (United States)

    Castellanos, M. T.; Cartagena, M. C.; Ribas, F.; Cabello, M. J.; Arce, A.; Tarquis, A. M.

    2010-05-01

    Provided that the intensive use of N in agricultural systems has negatively impacted environmental quality, because a higher fertilization requirements of the crop to make the nitrate leached into the aquifer contaminating, as well as increasing their presence on the ground resulting in a time of intercropping nitrates washing with the arrival of rains. It's necessary to know the N management to reduce the pollution risks and may lead the higher production with the fewer economic cost, environmental and health. The aim of this study was to asses how different doses of nitrogen applied to a melon crop affected the quality of groundwater. The doses of N applied ranged between 11 and 393 kg ha-1. Several indexes, based in N leaching consequence of the agricultural practice, were calculated and provided an essential tool for knowing the risks of groundwater pollution with the practices used. Drinking water impact, irrigation water management impact and environmental impact indexes were obtained during four years in a vulnerable area of the centre of Spain. Acknowledgements: This project has been supported by INIA-RTA04-111

  6. Descripción de tres nuevas especies de tetigónidos de Costa Rica (Orthoptera, Tettigoniidae

    Directory of Open Access Journals (Sweden)

    Barranco Vega, P.

    2013-12-01

    Full Text Available Description of three new species of katydids from Costa Rica (Orthoptera, Tettigoniidae Three new species of katydids from the Reserva Biológica Alberto Manuel Brenes (Alajuela in Costa Rica (Central America are described. The specimens were collected in the course of the Project INVARTRAB developed by the Central University of Costa Rica and the INIA de Madrid (España. The specimens were sampled by direct prospection on plants and light traps. The new species belong to the genera Phlugis, Scopiorinus and Viadana, respectively. Phlugis ramirezi n. sp. has little developed tegmens and wings, male tenth abdominal tergite ending in two spiniform processes, cerci curved with a basal spur, and four teeth in the apex. Scopiorinus estebani n. sp. shows male tenth abdominal tergite with a longer than broad expansion, subgenital plate depressed in the middle, and lateral spiniform processes at the end. Viadana rowelli n. sp. has rhomboidal tegmens and male cerci with an internal branch with serrulated apex inserted in the last third. Main characters are illustrated and compared in related species in each genus.

  7. Caracterización bioquímica de hojas de clones de Theobroma cacao y su relación con los tricomas

    Directory of Open Access Journals (Sweden)

    Janet Quiñones Galvez

    2015-11-01

    Full Text Available Theobroma cacao L. is a crop of major importance for their potential applications in pharmacy and agriculture. It contains metabolites that are of great interest for its natural biological functions in plant response to different types of stress, both biotic and abiotic. The presence, type and concentration of both primary and secondary metabolites may vary depending on the genotype analyzed and these compounds can accumulate in the architecture associated structures such as leaf trichomes. The aim of this study was to characterize 26 clones of the germplasm bank of Padrón, INIA-Miranda, Miranda State, Venezuela and study the relationship between biochemical indicators assessed and the number of trichomes. The content of phenolic compounds, soluble protein, free proline, total carbohydrates, lignins, the number of trichomes and the relationship between all traits was determined. The results showed that in the two dimensional space explained 46.68% of the variability, five homogeneous groups were defined: 10 clones were associated with the maximum gradient of proline, total carbohydrate and lignin content. Nine clones were associated with high content of phenols and a group of seven clones with the highest number of trichomes on leaves. It was found that the number of trichomes was not related to the concentration of phenolic compounds. The clones of Forastero cocoa type showed higher concentration of total phenols and carbohydrates, meanwhile the Trinitario type presented higher concentration of proteins and prolina.

  8. [Soluble and insoluble dietary fiber in cereals and legumes cultivated in Chile].

    Science.gov (United States)

    Pak, N; Ayala, C; Vera, G; Pennacchiotti, I; Araya, H

    1990-03-01

    Insoluble, soluble and total dietary fiber (DF) were determined in 35 varieties of certified whole seeds (without processing) of cereals (rice, oat, rye, and wheat) and legumes (pea, cowpea, beans, chikpea, lentil and lupine). The enzymatic method of Asp, Johansson and Siljestrom was used, with modifications in relation to time of incubation with alpha amylase, filtration system and volumes of the filtrates. Results were expressed as g/100 g dry weight. Total DF for cereals showed a range from 10.1 (wheat var. Chasqui) to 22.2 (rice var Quella). Rye, var. Tetra Baer and oats var. Pony Baer presented the highest soluble fiber content (3.3 and 3.9, respectively). In legumes, total DF fluctuated between 12.7 (pea, var. yellow) and 36.6 (lupine, var. Multolupa). Bean, var. Pinto INIA and lupine var. Multolupa presented the highest soluble fiber values (5.8 for both). Based on the results of this research work, it might be concluded that great variation exists in regard to the amount of total soluble and insoluble DF in cereals and legumes, a fact which impedes generalization as to its content in each food item.

  9. Relationship between the nutrition status and sensory characteristics of melon fertilized with wine-distillery waste compost

    Science.gov (United States)

    Requejo, María Isabel; Sánchez-Palomo, Eva; González, Miguel Angel; Castellanos, Maria Teresa; Villena, Raquel; Cartagena, Maria Carmen; Ribas, Francisco

    2015-04-01

    The interest in developing sustainable agriculture is becoming more important day by day. A large quantity of wastes from the wine and distillery industry are produced and constitute a serious problem not only environmental but also economic. The use of exhausted grape marc compost as organic amendment is a management option of the fertility of soils. On the other hand, consumers are increasingly concerned about the type, quality and origin of food production. Flavor and aroma are most often the true indicators of shelf-life from the consumer's point of view. The aim of this study was to relate the nutritional status of melon fertilized with exhausted grape marc compost with the sensory profile of fresh-cut fruits. A field experiment was established with three doses of compost (1, 2 and 3 kg per linear meter) and a control. Melons were harvested at maturity and the sensory evaluation was carried out by an expert panel of melon tasters to describe odour, flavour and texture. Nitrogen, phosphorus and potassium concentration was determined in the fruits to calculate nutrient absorption. Acknowledgements: This project has been supported by INIA-RTA2010-00110-C03-01

  10. Culinary and sensory traits diversity in the Spanish Core Collection of common beans (Phaseolus vulgaris L.)

    Energy Technology Data Exchange (ETDEWEB)

    Rivera, A.; Casquero, P.A.; Mayo, S.; Almirall, A.; Plans, M.; Simó, J.; Romero-del-Castillo, R.; Casañas, F.

    2016-11-01

    The Spanish National Plant Genetic Resource Center’s core collection of bean germplasm includes 202 accessions selected from more than 3000 accessions in function of passport data, seed phenotype, genetic background, and agronomic traits. To acquire more useful information about these accessions, we cultivated and characterized them for sensory and culinary traits. We found considerable variation for culinary and sensory traits of the cooked beans (mean coefficients of variation: 41% for the sensory traits and 40% for the culinary traits). The large dataset enabled us to study correlations between sensory and culinary traits and among these traits and geographic origin, seed color, and growth habit. Greater proportion of white in the seed coat correlated positively with brightness and negatively with mealiness (r=0.60, r=-0.60, p<0.001, respectively). Mealiness correlated negatively with seed-coat roughness and rate of water absorption (r=-0.60, r=-0.53, p<0.001, respectively). Materials of Andean origin had lower seed-coat brightness (p<0.01) and seed-coat roughness, and greater seed-coat perceptibility, mealiness, flavor, and aroma (p<0.001) than materials of Mesoamerican origin. Growth habit failed to correlate with culinary or sensory traits. Breeders can benefit from the information about this core collection available at www.crf.inia.es/crfesp/paginaprincipaljudia.asp. (Author)

  11. Glyceradehyde-3-phosphate dehydrogenase as a suitable vaccine candidate for protection against bacterial and parasitic diseases.

    Science.gov (United States)

    Perez-Casal, Jose; Potter, Andrew A

    2016-02-17

    The enzyme glyceraldehyde-3-P-dehydrogenase (GAPDH) has been identified as having other properties in addition to its key role in glycolysis. The ability of GAPDH to bind to numerous extracellular matrices, modulation of host-immune responses, a role in virulence and surface location has prompted numerous investigators to postulate that GAPDH may be a good vaccine candidate for protection against numerous pathogens. Although immune responses against GAPDH have been described for many microorganisms, vaccines containing GAPDH have been successfully tested in few cases including those against the trematode-Schistosoma mansoni, the helminth-Enchinococcus multilocularis; the nematode filaria- Litomosoides sigmodontis; fish pathogens such as Aeromonas spp., Vibrio spp., Edwarsiella spp., and Streptococcus iniae; and environmental streptococci, namely, Streptococcus uberis and Streptococcus dysgalactiae. Before GAPDH-based vaccines are considered viable options for protection against numerous pathogens, we need to take into account the homology between the host and pathogen GAPDH proteins to prevent potential autoimmune reactions, thus protective GAPDH epitopes unique to the pathogen protein must be identified.

  12. SKIN MUCUS OF RABBITFISH (SIGANUS ORAMIN) IS LETHAL TO CRYPTOCARYON IRRITANS AND SOME OTHER PATHOGENIC ORGANISMS%黄斑蓝子鱼皮肤黏液对刺激隐核虫及一些病原菌的抑杀作用

    Institute of Scientific and Technical Information of China (English)

    黎睿君; 刘芳; 王方华; 李安兴

    2013-01-01

    Cryptocaryon irritans, an important marine ciliate protozoan that causes a disease known as "marine white spot disease", has already become one of the most harmful parasites in Chinese marine aquaculture industry. Previous studies indicated that the rabbitfish (Siganus oramin) showed low susceptibility to C. irritans infections, and the serum of rabbitfish had killing effect against C. irritans. Practically, the skin mucus of fish has been considered to be the first defense against infections caused by various pathogens. Whether the skin mucus of rabbitfish has the same parasiticidal activity is the main research purpose of this paper. In this study, we compared the antiparasitic and antibacterial activities of skin mucus from S. oramin, Epinephelus coioides, Sciaemops ocellatus, Plectorhinchus cinctus and Trachinotus ovatus. The parasite-killing effects of skin mucus were tested by immobilization assay for three species of parasites, C. irritans, I. multifiliis, and T. brucei brucei. The antimicrobial properties of skin mucus were determined using the agar diffusion method and the minimum inhibitory concentration for six species of bacteria (Staphylo coccus aureus, Bacillus subtilis, Streptococcus iniae, Escherichia coli, Vibrio cholera, Aeromonas sobria, Edwardsiella tarda, Photobacterium damselae subsp. and Vibrio alginolyticus) and two species of fungi (Aspergillus niger and Gibberella saubinetii) were also examined. We found that compared with the skin mucus isolated from E. coioides, S. ocellatus, P. cinctus and T. ovatus, the rabbitfish skin mucus was lethal to C. irritans, I. multifiliis, and T. brucei brucei. The minimum parasiticidal concentration (MPC) of rabittfish skin mucus for C. irritans, I. multifiliis and T. brucei brucei were 4.0, 5.0, and 3.0 mg/mL, respectively. After the theronts of C. irritans and /. multifiliis were co-incubated with the skin mucus of rabbit-fish, we observed the cilia falling off, body swelling, outer membrane rupture and

  13. Prevention of late blowing defect by reuterin produced in cheese by a Lactobacillus reuteri adjunct.

    Science.gov (United States)

    Gómez-Torres, Natalia; Ávila, Marta; Gaya, Pilar; Garde, Sonia

    2014-09-01

    In this study, reuterin-producing Lactobacillus reuteri INIA P572 was added to cheese as an adjunct culture together with 50 or 100 mM glycerol (required for reuterin production), with the aim of controlling Clostridium tyrobutyricum CECT 4011 growth and preventing the late blowing defect (LBD) of cheese caused by this strain. L. reuteri survived cheese manufacture and produced reuterin in situ, detected at 6 and 24 h. However, the produced reuterin was enough to inhibit the growth of Clostridium, showing undetectable spore counts from day 30 onward and, therefore, to prevent cheese LBD during ripening (60 d, 14 °C). The acidification of these cheeses was not affected, although from day 14 they showed significantly lower lactococci counts than cheese made only with the starter (control cheese). Cheeses with LBD showed lower levels of lactic acid than control cheese and the formation of propionic and butyric acids, but cheeses with reuterin showed the same organic acids profile than control cheese. The cheese made with L. reuteri and 100 mM glycerol showed a light pink colour, not observed in the cheese made with L. reuteri and 50 mM glycerol. These results demonstrated a potent anti-clostridial activity of reuterin produced in an actual food product like cheese, and proved to be a novel approach to prevent LBD of cheese.

  14. 高良姜与大高良姜精油中活性物质的比较

    Institute of Scientific and Technical Information of China (English)

    张倩芝; 蔡明招; 卢志毅

    2006-01-01

    姜科植物是分布于热带和亚热带药材中的重要家族,姜科分为2亚科、3族,约49属、1500种,包括姜黄Curcuma longa L.、小豆寇Elettaria cardamomum(Roxb.)Maton、生姜Zingiber officinale ROSC.、莪术Curcuma zedoaria(Berg.)Rosc.、大高良姜Alpinia galanga(L.)Willd.、益智A珍inia oxyphylla Miq.和山柰Kaempferia galanga Linn.等。姜科植物主产地为亚洲热带,我国有19属,150余种,分布于东南至西南部各省区,是东南亚各国及我国人民日常生活中常用的植物。

  15. Characterization of native strains of Azospirillum spp. and its effect on growth of Oryza sativa L. “rice” in Lambayeque

    Directory of Open Access Journals (Sweden)

    Franklin García

    2010-06-01

    Full Text Available This research was conducted to characterize and determine the effect of native strains of Azospirillum spp. on growth of Oryza sativa L. "rice" as an alternative for the reduction of chemical fertilizers. The bacteria were isolated from roots of rice in Lambayeque. Fragments of previously disinfected roots were planted in Nfb semisolid medium, were nitrogen-fixing bacteria were recognized by a whitish film on the surface and turn from green to blue. The genus Azospirillum was identified in Congo red agar medium, obtained 96 isolates with production of 2.69 to 38.02 ppm indoleacetic acid and 7.95 to 29.09 ppm of fixed nitrogen. The four strains with the highest values were inoculated independently and in combination with 50 and 100 % urea in Tinajones INIA508 rice. It used a completely randomized design and including witnesses’ 50 and 100 % urea and absolute control. All native strains increased height and dry matter of shoot and roots, with effectiveness index of up to 21.77, 102.06 and 126.11 % compared to untreated control. Azospirillum sp. GM-86 selected by the highest value of indoleacetic acid, reached the highest level of effectiveness in height and root dry biomass. The application of 50 % úrea with Azospirillum spp. increased rates of effectiveness. It demonstrated the potential of native strains of Azospirillum spp. to increase the growth of rice as well as the possibility to use associate with low doses of urea.

  16. Development of real-time PCR for detection and quantitation of Streptococcus parauberis.

    Science.gov (United States)

    Nguyen, T L; Lim, Y J; Kim, D-H; Austin, B

    2016-01-01

    Streptococcus parauberis is an increasing threat to aquaculture of olive flounder, Paralichthys olivaceus Temminck & Schlegel, in South Korea. We developed a real-time polymerase chain reaction (PCR) method using the TaqMan probe assay to detect and quantify S. parauberis by targeting the gyrB gene sequences, which are effective for molecular analysis of the genus Streptococcus. Our real-time PCR assay is capable of detecting 10 fg of genomic DNA per reaction. The intra- and interassay coefficient of variation (CV) values ranged from 0.42-1.95%, demonstrating that the assay has good reproducibility. There was not any cross-reactivity to Streptococcus iniae or to other streptococcal/lactococcal fish pathogens, such as S. agalactiae and Lactococcus garvieae, indicating that the assay is highly specific to S. parauberis. The results of the real-time PCR assay corresponded well to those of conventional culture assays for S. parauberis from inoculated tissue homogenates (r = 0.957; P real-time PCR is a valuable tool for diagnostic quantitation of S. parauberis in clinical samples.

  17. First description of programmed cell death10 (PDCD10) in rock bream (Oplegnathus fasciatus): Potential relations to the regulation of apoptosis by several pathogens.

    Science.gov (United States)

    Kim, Ju-Won; Jeong, Ji-Min; Bae, Jin-Sol; Cho, Dong-Hee; Jung, Sung Hee; Hwang, Jee-Youn; Kwon, Mun-Gyeong; Seo, Jung Soo; Baeck, Gun-Wook; Park, Chan-Il

    2016-02-01

    In this study, we isolated and characterized programmed cell death10 (PDCD10), which is known to be related to apoptosis, from rock bream (Oplegnathus fasciatus). The full-length rock bream PDCD10 (RbPDCD10) cDNA (1459 bp) contains an open reading frame of 633 bp that encodes 210 amino acids. Furthermore, multiple alignments revealed that the six of the α-helix bundles were well conserved among the other PDCD10 sequences tested. RbPDCD10 was significantly expressed in the liver, RBC (red blood cell), gill, intestine, trunk kidney and spleen. RbPDCD10 gene expression was also examined in several tissues, including the kidney, spleen, liver, and gill, under bacterial and viral challenges. Generally, all of the examined tissues from the fish that were infected with Edwardsiella tarda and the red sea bream iridovirus (RSIV) exhibited significant up-regulations of RbPDCD10 expression compared to the controls. However, RbPDCD10 expression exhibited dramatic down-regulations in all of the examined tissues following injections of Streptococcus iniae, which is major bacterial pathogen that is responsible for mass mortality in rock bream. Our results revealed that rock bream PDCD10 may be involved in the apoptotic regulation of rock bream immune responses.

  18. New insight into bacterial zoonotic pathogens posing health hazards to humans

    Directory of Open Access Journals (Sweden)

    Marcin Ciszewski

    2014-12-01

    Full Text Available This article presents the problem of evolutionary changes of zoonotic pathogens responsible for human diseases. Everyone is exposed to the risk of zoonotic infection, particularly employees having direct contact with animals, i.e. veterinarians, breeders, butchers and workers of animal products’ processing industry. The article focuses on pathogens monitored by the European Centre for Disease Prevention and Control (ECDC, which has been collecting statistical data on zoonoses from all European Union countries for 19 years and publishing collected data in annual epidemiological reports. Currently, the most important 11 pathogens responsible for causing human zoonotic diseases are being monitored, of which seven are bacteria: Salmonella spp., Campylobacter spp., Listeria monocytogenes, Mycobacterium bovis, Brucella spp., Coxiella burnetti and Verotoxin- producing E. coli (VTEC / Shiga-like toxin producing E. coli (STEC. As particularly important are considered foodborne pathogens. The article also includes new emerging zoonotic bacteria, which are not currently monitored by ECDC but might pose a serious epidemiological problem in a foreseeable future: Streptococcus iniae, S. suis, S. dysgalactiae and staphylococci: Staphylococcus intermedius, S. pseudintermedius. Those species have just crossed the animal-human interspecies barrier. The exact mechanism of this phenomenon remains unknown, it is connected, however, with genetic variability, capability to survive in changing environment. These abilities derive from DNA rearrangement and horizontal gene transfer between bacterial cells. Substantial increase in the number of scientific publications on this subject, observed over the last few years, illustrates the importance of the problem. Med Pr 2014;65(6:819–829

  19. Antimicrobial activity of reuterin produced by Lactobacillus reuteri on Listeria monocytogenes in cold-smoked salmon.

    Science.gov (United States)

    Montiel, R; Martín-Cabrejas, I; Langa, S; El Aouad, N; Arqués, J L; Reyes, F; Medina, M

    2014-12-01

    Lactobacillus reuteri INIA P579 was used for the production and purification of reuterin. The purity of reuterin was assessed by high resolution electrospray ionization mass spectrometry (HRESIMS) and nuclear magnetic resonance (NMR) spectroscopy. After purification, reuterin concentration obtained was 1.3 M. The inhibitory activity using Escherichia coli K12 as indicator strain was estimated to be 510 AU/ml. Survival curves in tryptic soy broth revealed that reuterin required to inhibit the growth of three Listeria monocytogenes strains was in the range of 2-4 AU/ml. Purified reuterin (10 AU/g) significantly reduced the growth of L. monocytogenes in cold-smoked salmon kept under moderate or strong temperature abuse conditions. After 15 d at 8 °C, cold-smoked salmon with added reuterin exhibited L. monocytogenes counts 2.0 log CFU/g lower than control smoked salmon with no reuterin added. At 30 °C, reuterin also controlled the growth of the pathogen, with counts 1.4 and 0.9 log CFU/g lower than those observed in control smoked salmon after 24 and 48 h, respectively. The addition of purified reuterin might be used as a hurdle technology to improve the safety and extend the shelf-life of lightly preserved seafood products such as cold-smoked salmon.

  20. Hemato-Immunological Responses and Disease Resistance in Siberian Sturgeon Acipenser baerii Fed on a Supplemented Diet of Lactobacillus plantarum.

    Science.gov (United States)

    Pourgholam, Moheb Ali; Khara, Hossein; Safari, Reza; Sadati, Mohammad Ali Yazdani; Aramli, Mohammad Sadegh

    2017-03-01

    A feeding trial was conducted to investigate the effects of different levels of dietary Lactobacillus plantarum on hemato-immunological parameters and resistance against Streptococcus iniae infection in juvenile Siberian sturgeon Acipenser baerii. Fish (14.6 ± 2.3 g) were fed three experimental diets prepared by supplementing a basal diet with L. plantarum at different concentrations [1 × 10(7), 1 × 10(8) and 1 × 10(9) colony-forming units (cfu) g(-1)] and a control (non-supplemented basal) diet for 8 weeks. Innate immune responses (immunoglobulin (Ig), alternative complement activity (ACH50) and lysozyme activity) were significantly higher in fish fed the 1 × 10(8) and 1 × 10(9) cfu g(-1) L. plantarum diet compared to the other groups (P white blood cell (WBC) and monocyte compared to those of the control group (P sturgeon juvenile.

  1. Depletion of florfenicol amine in tilapia (Oreochromis sp.) maintained in a recirculating aquaculture system following Aquaflor®-medicated feed therapy

    Science.gov (United States)

    Gaikowski, Mark P.; Whitsel, Melissa K.; Charles, Shawn; Schleis, Susan M.; Crouch, Louis S.; Endris, Richard G.

    2015-01-01

    Aquaflor® [50% w w−1 florfenicol (FFC)], is approved for use in freshwater-reared warmwater finfish which include tilapia Oreochromis spp. in the United States to control mortality from Streptococcus iniae. The depletion of florfenicol amine (FFA), the marker residue of FFC, was evaluated after feeding FFC-medicated feed to deliver a nominal 20 mg FFC kg−1 BW d−1 dose (1.33× the label use of 15 mg FFC kg−1 BW d−1) to Nile tilapia O. niloticus and hybrid tilapia O. niloticus × O. aureus held in a recirculating aquaculture system (RAS) at production-scale holding densities. Florfenicol amine concentrations were determined in fillets taken from 10 fish before dosing and from 20 fish at nine time points after dosing (from 1 to 240 h post-dosing). Water samples were assayed for FFC before, during and after the dosing period. Parameters monitored included daily feed consumption and biofilter function (levels of ammonia, nitrite and nitrate). Mean fillet FFA concentration decreased from 13.77 μg g−1 at 1-h post dosing to 0.39 μg g−1 at 240-h post dosing. Water FFC concentration decreased from a maximum of 1400 ng mL−1 at 1 day post-dosing to 847 ng mL−1 at 240 h post-dosing. There were no adverse effects noted on fish, feed consumption or biofilter function associated with FFC-medicated feed administration to tilapia.

  2. Culinary and sensory traits diversity in the Spanish Core Collection of common beans (Phaseolus vulgaris L.

    Directory of Open Access Journals (Sweden)

    Pedro A. Casquero

    2016-03-01

    Full Text Available The Spanish National Plant Genetic Resource Center’s core collection of bean germplasm includes 202 accessions selected from more than 3000 accessions in function of passport data, seed phenotype, genetic background, and agronomic traits. To acquire more useful information about these accessions, we cultivated and characterized them for sensory and culinary traits. We found considerable variation for culinary and sensory traits of the cooked beans (mean coefficients of variation: 41% for the sensory traits and 40% for the culinary traits. The large dataset enabled us to study correlations between sensory and culinary traits and among these traits and geographic origin, seed color, and growth habit. Greater proportion of white in the seed coat correlated positively with brightness and negatively with mealiness (r=0.60, r=-0.60, p<0.001, respectively. Mealiness correlated negatively with seed-coat roughness and rate of water absorption (r=-0.60, r=-0.53, p<0.001, respectively. Materials of Andean origin had lower seed-coat brightness (p<0.01 and seed-coat roughness, and greater seed-coat perceptibility, mealiness, flavor, and aroma (p<0.001 than materials of Mesoamerican origin. Growth habit failed to correlate with culinary or sensory traits. Breeders can benefit from the information about this core collection available at www.crf.inia.es/crfesp/paginaprincipaljudia.asp.

  3. MORPHOPHYSIOLOGICAL CHANGES OF IPOMOEA BATATAS (L. LAM. DURING DROUGHT STRESS

    Directory of Open Access Journals (Sweden)

    La Rosa, R.

    2008-01-01

    Full Text Available One of the greatest problems in the world is desertification so we must to know physiological responses and morphological adaptations from species with tolerance to drought stress. Sweet potato (Ipomoea batatas Lam. is one of the most important crops of the world, especially because of its great tolerance to drought stress. Hence, we decided to know what morphophysiological and metabolic responses has I. batatas under drought stress. So, we got cuttings of variety INA-100, from INIA Huaral, and were planted in 22 recipients of 4 L with substrate consisting in a mixing of sand and humus (3:1. Half of them were irrigated monthly and other half only receives water to establish plants and no more after that. We measured transpiration, histological changes in leaves and metabolic changes at protein and starch level. We confirmed transpiration is not affected for air humidity or substrate humidity. But, the effect of drought can be better observed in foliage growth, therefore in histological changes in leaves. No significance in concentration of proteins but significance in carbohydrates concentration shows an osmotic regulation in order to maintain stomata open and also an increase in photosynthetic activity in stressed plants.

  4. 淡水豚类线粒体DNA 12S rRNA基因的序列变异及其分子系统学研究%SEQUENCE VARIATION OF MITOCHONDRIAL 12S rRNA GENE AND INFERRED MOLECULAR SYSTEMATICS OF RIVER DOLPHINS

    Institute of Scientific and Technical Information of China (English)

    杨光; 刘珊

    2000-01-01

    淡水豚类4个代表属[白暨豚(Lipotes)、恒河豚(Platanista)、弗西豚(Pontoporia)和亚河豚(Inia)]mtDNA 12S rRNA基因的序列差异水平,高于其他齿鲸类科间的差异,特别是远远高于海豚总科内的科间差异.研究结果支持它们应归属于不同的科,即白暨豚科(Lipotiidae)、恒河豚科(Platanistidae)、弗西豚科(Pontoporidae)和亚河豚科(Iniidae).恒河豚科是鲸类中最早分化出来的独立支系,而其余3个淡水豚科组成一个单系,作为海豚总科的姊妹群.淡水豚类的发生是多系的.白暨豚科、亚河豚科和弗西豚科是否隶属于同一个总科尚需进一步研究.

  5. Complete amino acid sequence of the myoglobin from the Atlantic bottlenosed dolphin, Tursiops truncatus.

    Science.gov (United States)

    Jones, B N; Vigna, R A; Dwulet, F E; Bogardt, R A; Lehman, L D; Gurd, F R

    1976-10-05

    The complete amino acid sequence of the major component myoglobin from the Atlantic bottlenosed dolphin, Tursiops truncatus, was determined by specific cleavage of the protein to obtain large peptides that are readily degraded by the automatic sequencer. Three easily separable peptides were obtained by cleaving the protein with cyanogen bromide at the 2 methionine residues and 4 peptides were obtained by cleaving the methyl acetimidated protein with trypsin at the 3 arginine residues. By subjecting 4 of these peptides and the apomyoglobin to automatic Edman degradation, over 80% of the covalent structure of the protein was obtained. The remainder of the primary structure was determined by further digestion of the central cyanogen bromide peptide with trypsin and staphylococcal protease. This myoglobin differs from that of the sperm whale, Physter catodon, at 15 positions, from that of the California gray whale, Eschrichtius gibbosus, at 14 positions, from that of the common porpoise, Phocoena phocoena, at 6 positions, and from the myoglobin of the Black Sea dolphin, Delphinus delphis and the Amazon River dolphin, Inia goeffrensis, at 5 and 7 positions, respecitvely. All substitutions observed in this sequence fit easily into the tertiary structure of sperm whale myoglobin.

  6. Bacterial zoonoses of fishes: a review and appraisal of evidence for linkages between fish and human infections.

    Science.gov (United States)

    Gauthier, David T

    2015-01-01

    Human contact with and consumption of fishes presents hazards from a range of bacterial zoonotic infections. Whereas many bacterial pathogens have been presented as fish-borne zoonoses on the basis of epidemiological and phenotypic evidence, genetic identity between fish and human isolates is not frequently examined or does not provide support for transmission between these hosts. In order to accurately assess the zoonotic risk from exposure to fishes in the context of aquaculture, wild fisheries and ornamental aquaria, it is important to critically examine evidence of linkages between bacteria infecting fishes and humans. This article reviews bacteria typically presented as fish-borne zoonoses, and examines the current strength of evidence for this classification. Of bacteria generally described as fish-borne zoonoses, only Mycobacterium spp., Streptococcus iniae, Clostridium botulinum, and Vibrio vulnificus appear to be well-supported as zoonoses in the strict sense. Erysipelothrix rhusiopathiae, while transmissible from fishes to humans, does not cause disease in fishes and is therefore excluded from the list. Some epidemiological and/or molecular linkages have been made between other bacteria infecting both fishes and humans, but more work is needed to elucidate routes of transmission and the identity of these pathogens in their respective hosts at the genomic level.

  7. Respuesta de la madera ante el fuego en la construcción

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    Elvira Martín, Luis Miguel

    1984-03-01

    Full Text Available Not available.El presente artículo pretende dar una amplia visión del material madera y de los elementos que la utilizan como materia prima (tableros, puertas, etc., cuando se encuentran sometidos a la acción del fuego. Se hace un análisis partiendo de la base de realización de ensayos a partir de la Normativa española al respecto, considerando las especies más frecuentemente empleadas en nuestro país, y abarcando los aspectos de duración cortafuegos, estabilidad mecánica sin deformación y estanquidad a las llamas, sin olvidar la emisión de gases inflamables y clasificación frente al fuego. A través de la experiencia que el Laboratorio del Fuego del INIA posee, y que se exponen en esta colaboración, se pueden extraer consecuencias que permitan la utilización de la madera en construcción de una forma más lógica, eliminando algunos prejuicios que existen sobre su naturaleza y comportamiento frente al fuego.

  8. Identification of Bacillus strains for biological control of catfish pathogens.

    Science.gov (United States)

    Ran, Chao; Carrias, Abel; Williams, Malachi A; Capps, Nancy; Dan, Bui C T; Newton, Joseph C; Kloepper, Joseph W; Ooi, Ei L; Browdy, Craig L; Terhune, Jeffery S; Liles, Mark R

    2012-01-01

    Bacillus strains isolated from soil or channel catfish intestine were screened for their antagonism against Edwardsiella ictaluri and Aeromonas hydrophila, the causative agents of enteric septicemia of catfish (ESC) and motile aeromonad septicaemia (MAS), respectively. Twenty one strains were selected and their antagonistic activity against other aquatic pathogens was also tested. Each of the top 21 strains expressed antagonistic activity against multiple aquatic bacterial pathogens including Edwardsiella tarda, Streptococcus iniae, Yersinia ruckeri, Flavobacterium columnare, and/or the oomycete Saprolegnia ferax. Survival of the 21 Bacillus strains in the intestine of catfish was determined as Bacillus CFU/g of intestinal tissue of catfish after feeding Bacillus spore-supplemented feed for seven days followed by normal feed for three days. Five Bacillus strains that showed good antimicrobial activity and intestinal survival were incorporated into feed in spore form at a dose of 8×10(7) CFU/g and fed to channel catfish for 14 days before they were challenged by E. ictaluri in replicate. Two Bacillus subtilis strains conferred significant benefit in reducing catfish mortality (Pbiological control in vivo was also investigated in terms of whether the strains contain plasmids or express resistance to clinically important antibiotics. The Bacillus strains identified from this study have good potential to mediate disease control as probiotic feed additives for catfish aquaculture.

  9. Evaluación del rendimiento de grano seco en accesiones promisorias de Plukenetia volubilis “sacha inchi” en Loreto

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    Andrés Fernández Sandoval

    2013-06-01

    Full Text Available El sacha inchi es una especie vegetal oleaginosa que se encuentra al estado silvestre en selva alta, baja y ceja de selva del Perú. Es importante por su alto contenido de ácidos grasos insaturados (aceites omegas y proteínas que contienen las semillas que lo hace ideal para mejorar la dieta alimenticia humana. El Gobierno Regional ha priorizado el cultivo de sacha inchi para impulsar el desarrollo económico y social de sus productores. Los trabajos de evaluación de rendimiento de grano seco de sacha inchi bajo condiciones de selva baja, se realizaron en el Campo Experimental El Dorado de la EEA. San Roque- INIA; con 9 accesiones provenientes del banco de germoplasma de la EEA. El Porvenir, Tarapoto. El objetivo de esta investigación fue evaluar el rendimiento de grano seco y validar y adaptar la tecnología de producción de esta especie en condiciones de selva baja. Las accesiones que sobresalieron fueron Barranquita y Cumbaza, con rendimientos de 1863 y 1809 kg/ha de grano seco por hectárea. La accesión Tambo Yaguas, obtuvo el rendimiento más bajo con 631 kg/ha de grano seco por hectárea, debido principalmente a su susceptibilidad a Rhizoctonia sp. “Mustia hilachoza”.

  10. Anomalias ósseas congênitas em Sotalia guianensis (Mammalia, Cetacea, Delphinidae da costa centro-norte do Estado do Rio de Janeiro Congenital bone defects in Guiana dolphin, Sotalia guianensis (Mammalia, Cetacea, Delphinidae in the northcentral coast of Rio de Janeiro State, Brazil

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    Maíra Laeta

    2010-06-01

    Full Text Available Carcaças de botos-cinza Sotalia guianensis (van Bénéden, 1864 foram recuperadas entre 20 de agosto de 2001 e 13 de fevereiro de 2006, na costa centro-norte fluminense. A maior parte delas (85% entre Barra de São João (22º35'S 41º59'W e Quissamã (22º06'S 41º28'W. Foram estudadas as colunas vertebrais dos 20 exemplares que apresentavam mais de 60% das vértebras presentes. A coleção é formada por 75% de animais imaturos, e todos os esqueletos mostram alterações tafonômicas mínimas. Duas categorias de anomalias congênitas foram diagnosticadas, ambas relacionadas ao desenvolvimento do mesoderma paraxial. A sétima vértebra cervical (C7 foi a única afetada, as costelas cervicais e o não fechamento do arco neural atingiram 15 (75% dos indivíduos analisados, dos quais três (15% apresentam ambas as anomalias. Nove (45% indivíduos apresentaram costelas cervicais uni ou bilateral, e nove (45% indivíduos apresentaram não fechamento do arco neural; em todos os casos as vértebras contíguas eram normais. A ocorrência de anomalias nesta série do Rio de Janeiro é maior do que as referidas na literatura brasileira para outras séries de Sotalia do Amazonas, Ceará e Santa Catarina. A série de Sotalia descrita vem de uma região do litoral muito limitada e provavelmente representa uma população local. As costelas cervicais são geneticamente determinadas e podem estar concentradas por uma condição de grande proximidade biológica entre os animais; o não fechamento do arco pode ter também um componente ambiental, a ser investigado futuramente.Carcasses of Guiana dolphins, Sotalia guianensis (van Bénéden, 1864 were recovered from August 20, 2001 to February 13, 2006 along the north-central coast of Rio de Janeiro state. Most of the animals (85% were found between Barra de São João (22º35'S 41º59'W and Quissamã (22º06'S 41º28'W. We studied the vertebral columns of all the 20 specimens that had more than 60% of the

  11. Adaptación al cambio climático y la variabilidad: algunas opciones de respuesta para la producción agrícola en Uruguay Adaptation to climatic change and variability: some response options to agricultural production in Uruguay

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    Agustín Giménez

    2012-06-01

    Full Text Available Adaptación al cambio climático y la variabilidad: "algunas opciones de respuesta para la producción agrícola en Uruguay". Como se señala en varios informes del Panel Intergubernamental sobre Cambio Climático (IPCC, 2007a; IPCC, 2007b, la comunidad científica internacional expresa que el aumento en la concentración de gases de efecto invernadero tiene como resultado cambios en la variabilidad climática diaria, estacional, interanual y a lo largo de decenios. La variabilidad climática y la ocurrencia de eventos extremos (heladas, granizos, sequías resulta en perjuicios muy importantes para el sector agropecuario y frecuentemente el sector requiere de varios años para recuperarse económica y financieramente de los daños ocasionados. La unidad de agroclima y sistemas de información (GRAS del Instituto Nacional de Investigación Agropecuaria (INIA de Uruguay en conjunto con el Banco Mundial (BM y la Universidad de Cornell (UC de los Estados Unidos de América, finalizaron en el año 2009 el proyecto "Vulnerabilidad al cambio climático en los sistemas de producción agrícola en América Latina y el Caribe: "desarrollo de respuestas y estrategias", (Giménez y Lanfranco, 2009. El objetivo de la propuesta fue formular un plan de acción con recomendaciones para el desarrollo de respuestas y estrategias con el fin de contribuir a una mejor adaptación a los impactos de la variabilidad climática y la ocurrencia de eventos climáticos extremos en los sistemas de producción agrícola de Uruguay. Como resultado del trabajo, se identificaron y priorizaron 3 opciones de respuesta: sistema de información y soporte para la toma de decisiones; gestión del agua; y seguros y otros instrumentos financieros para la gestión de riesgos.Adaptation to climatic change and variability: "some response options to agricultural production in Uruguay". Like is mentioned in several reports of Intergovernmental Panel about Climatic Change (IPCC, 2007a; IPCC

  12. Causas de mortalidad de alpacas en tres principales centros de producción ubicados en puna seca y humeda del departamento de Puno (Causes of mortality of alpacas in three main centers of production located in dry and humid fist of the Puno department

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    Mamani Paredes, Javier

    2009-08-01

    Full Text Available ResumenEl presente estudio se efectuó en tres principales centros de producción alpaquera del departamento de Puno: Rural Alianza EPS, CIP “La Raya” – UNA Puno ubicados en la zona agro ecológica de Puna húmeda y Anexo Quimsachata de INIA – Puno ubicado en la zona agro ecológica de Puna seca; con el objetivo de determinar las principales causas de mortalidad de alpacas. Mediante un estudio observacional analítico de tipo retrospectivo, utilizando los registros de mortalidad de las campañas de producción de 1998 – 2000. Determinándose que las principales causas de mortalidad dealpacas son: Agentes infecciosos 51.70%, anormalidades orgánicas24.08%, causas accidentales 13.36%, causas nutricionales 7.83% yenfermedades parasitarias 3.03%. Las causas infecciosas de mayorfrecuencia fueron: Las neumonías 31.12%, entero toxemia 20.90%,estomatitis 17.46% y otras en menor frecuencia. La mortalidad por causas orgánicas son la Caquexia de los animales 58.88%, aunque en puna seca la torsión intestinal y atonía ruminal son de mayor frecuencia. Se encontró como causas accidentales de muerte a: Traumatismos 50.30%, depredadores 28.63%, falsa deglución 7.35%, asfixia 7.16% y otros con menor frecuencia. Las principales causas nutricionales de mortalidad son:Desnutrición 27.12%, inanición 23.73%, falta de leche 19.32% y otras en menor proporción. Las causas parasitarias de mortalidad se determino: Sarna 33.33%, sarcosistiosis 28.95%, coccidiosis 25.44%, gastroenteritis verminosa 10.52%, dictiocaulosis e hidatidosis 0.88% respectivamente.SummaryThe present study took place in three main centers of alpaquera production of the department of Puno: Rural Alliance EPS, CIP “the Ray” - a Fist located in the zone ecological land of humid Fist and Quimsachata Annex of INIA - Fist located in the ecological zone land of dry Fist; with the aim of determining the main causes of mortality of alpacas. By means of an analytical observacional study

  13. Adaptability and Genotype x Environment Interaction of Spring Wheat Cultivars in Chile using Regression Analysis, AMMI, and SRAG Adaptabilidad e Interacción Genotipo x Ambiente en Trigos de Primavera utilizando Análisis de Regresión, AMMI y SREG

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    Dalma Castillo

    2012-06-01

    Full Text Available Wheat (Triticum aestivum L. genetic improvement objectives include obtaining cultivars capable of expressing their maximum potential yield and quality in diverse environments. This make necessary to know and define the environment in which a variety can express its maximum potential yield and quality. The objective of this study was to assess which method is the most efficient to study cultivars response in multiple environments. For this, we analyze the adaptability, stability, and genotype x environment (GxE interaction effect, grain yield, sedimentation, and wet gluten content of 13 spring wheat cultivars sown in six environments in the central-south and southern zones of Chile during two seasons. The data were analyzed by regression analysis, additive main effects and multiplicative interaction (AMMI, and the sites regression (SREG model. By this was thus established that SREG analysis is the most efficient for this type of study since, in addition to analyzing stability, adaptability, and effect (GxE, it allows identifying the best cultivar. In this case, 'Pandora-INIA' stands out by exhibiting the best yield (7.38 t ha-1, high sedimentation (36.95 cm³, and wet gluten (41.54% indices in all the environments, and this positions it as a variety having both high yield and quality.Dentro de los objetivos del mejoramiento genético del trigo (Triticum aestivum L. figura la obtención de variedades capaces de expresar su máximo potencial de rendimiento y calidad en diversos ambientes. Por lo cual es necesario conocer y definir el ambiente en que una variedad puede expresar al máximo su potencial de rendimiento y de calidad. El objetivo de este trabajo fue determinar una metodología eficiente para analizar la respuesta de diversos cultivares de trigo a distintos ambientes. Para ello se analizó la adaptabilidad, estabilidad y la interacción Genotipo x Ambiente (GxE de 13 variedades de trigo de primavera, sembradas, en seis ambientes de la

  14. Avaliação de espécies forrageiras de inverno na Depressão Central do Rio Grande do Sul Evaluation of cool season forage species in Depressão Central of Rio Grande do Sul

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    Marta Gomes da Rocha

    2007-12-01

    Full Text Available Um experimento foi conduzido para avaliar a produção de forragem de espécies e cultivares forrageiros de inverno provenientes do programa de seleção do Instituto Nacional de Investigación Agropecuária-INIA/Uruguai.Foram avaliadas as seguintes espécies: azevém (Lolium multiflorum Lam., cultivares Cetus, Estanzuela 284 e Titan; aveia (Avena byzantina, cultivares RLE 115a e 1095a; trevo-branco (Trifolium repens L., cv. Estanzuela Zapicán; trevo-vermelho (Trifolium pratense L., cv. Estanzuela 116; e cornichão (Lotus corniculatus L., cv. São Gabriel. Determinaram-se a produção de MS, o percentual e a produção dos componentes estruturais, a altura do dossel e os percentuais de FDN e PB. O delineamento experimental foi de blocos ao acaso com parcelas subdivididas no tempo, com quatro repetições. Entre as gramíneas, o azevém cv. Titan apresentou a maior produção de MS, com 7,2 t/ha e elevada proporção de folhas. As produções de cornichão e trevo-vermelho foram semelhantes e superiores às do trevo-branco em produção de MS. As espécies e os cultivares testados mostraram-se adaptados às condições locais, mesmo com o déficit hídrico durante o período experimental.The experiment was carried out to evaluate the herbage production of cool season forage species and cultivars from Instituto Nacional de Investigación Agropecuária- INIA/Uruguay breeding program. The following species and cultivars were evaluated: Italian ryegrass (Lolium multiflorum Lam.: Cetus, Estanzuela 284 and Titan; Oats (Avena byzantina: RLE 115a and 1095a; White clover (Trifolium repens L.: Estanzuela Zapicán; Red clover (Trifolium pratense L.: Estanzuela 116 and Birdsfoot trefoil (Lotus corniculatus L. São Gabriel. Dry matter production, structural components percentage and production, canopy height, neutral detergent fiber, and crude protein percentages were determined. It was used a split-plot arrangement in a randomized block design, where the

  15. Preliminary behavioural study of Caballo Fino Chilote stallions with restricted access to space and water during summer Estudio preliminar del comportamiento de potros raza Caballo Fino Chilote con acceso restringido a espacio y agua durante el verano

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    TA Tadich

    2010-01-01

    Full Text Available The Caballo Fino Chilote (CFCh is the only Chilean pony breed and a unique genetic resource. A group of seven CFCh stallions were observed for 60 hours, during summer, at INIA- Butalcura research station, Chiloé Island, under restricted space and access to water conditions. Scan sampling every five minutes was used to register 5 behavioural states and continuous focal sampling for 19 behavioural events. Inter-male interactions had a low frequency of presentation being avoidance (1.2 average events per stallion per day the most common. Overall stallions spent 55.89% (± 4.3% of their time foraging, 2.5% (± 0.82% in locomotion, 7.26% (± 0.82% standing alert, 32.24% (± 2.7% resting and 1.86% (± 0.64 in other behaviours. Foraging was the main activity, its occurrence was significantly lower (P El Caballo Fino Chilote (CFCh es la única raza de pony chileno y un recurso genético único. Un grupo de 7 potros CFCh fue observado por 60 horas, durante el verano, en la estación experimental del INIA-Butalcura, Isla de Chiloé, bajo condiciones de restricción de espacio y agua. El método de muestreo por escaneo se utilizó cada 5 minutos para evaluar estados conductuales y focal continuo para los eventos. Se registraron 5 estados y 19 eventos conductuales. Las interacciones entre potros tuvieron una frecuencia de presentación baja, siendo la más común “evasión” con un promedio de 1,2 eventos por potro por día. Los equinos utilizaron un 55,89% (± 4,3% del tiempo en forrajear, 2,5% (± 0,82% en locomoción, 7,26% (± 0,82% de pie alerta, 32,24% (± 2,7% descansando y 1,86% (± 0,64 en otras actividades. Forrajeo fue la principal actividad, siendo significativamente más baja (P < 0,05 durante el mediodía, periodo donde el descanso fue significativamente mayor (P < 0,05 comparado con los periodos de mañana y tarde. Se encontró una correlación positiva significativa entre temperatura y descanso y una correlación negativa significativa

  16. Caracterização isoenzimática de genótipos de arroz Isoenzymatic characterization of rice genotypes

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    Sandro Bonow

    2001-02-01

    Full Text Available Objetivou-se, neste trabalho, caracterizar isoenzimaticamente genótipos de arroz (Oryza sativa L.. A produtividade do arroz irrigado no Rio Grande do Sul é elevada, em virtude da alta tecnologia e sistema de irrigação usados, associados ao potencial alcançado pelas cultivares desenvolvidas através de melhoramento genético. Apenas seis ancestrais contribuem com 86% dos genes das cultivares mais plantadas. Como conseqüência desta estreita base genética, as cultivares apresentam um alto grau de parentesco e de similaridade de suas características morfológicas e agronômicas, o que dificulta a identificação varietal. A concorrência com genótipos, como arroz-vermelho e arroz-preto, de difícil controle por serem da mesma espécie que os cultivados, é considerada como um dos maiores problemas da cultura. Análises de isoenzimas podem ser usadas para o estudo da variabilidade e para estimar as relações genéticas existentes entre estes genótipos. Eletroforese em gel de poliacrilamida foi empregada, portanto, para caracterizar, através de isoenzimas de esterase, 6fosfogluconato desidrogenase, fosfoglucoisomerase e isocitrato desidrogenase em sementes e folhas de plântulas, e de fosfatase ácida e aspartato transaminase em folhas de plântulas, as cultivares BR-IRGA 409, BR-IRGA 410, BRS 6 ('Chuí', BRS 7 ('Taim', BRS Agrisul, INIA Taquari, El Paso L 144 e IRGA 417, e ecótipos de arroz-vermelho e arroz-preto. A análise de agrupamento, efetuada por meio do coeficiente de Jaccard e pelo método da média aritmética não ponderada (UPGMA, possibilitou a diferenciação de todos os genótipos, à exceção de BRS 6 ('Chuí' e BRS 7 ('Taim'. Três grupos foram identificados, incluindo-se, em um deles, os ecótipos de arroz-vermelho e arroz-preto, que apresentaram 95% de similaridade.The objective of this work was to characterize isoenzymaticly rice (Oryza sativa L. genotypes. Rice yields obtained in Rio Grande do Sul, Brazil, are high

  17. Evaluation of the Impact of Climatic Change on the Economic Value of Land in Agricultural Systems in Chile Evaluación del Impacto del Cambio Climático sobre el Valor Económico de la Tierra en Sistemas Agrícolas de Chile

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    Jorge González U

    2008-03-01

    Full Text Available Climatic change will affect crop yields and management. By the year 2050, the mean temperature could increase by 1.5 ºC; and by the year 2100 between 1.0 to 3.5 ºC. There are few studies on this subject in Chile. At the international level, estimated climatic changes in temperate and tropical zones could negatively affect wheat (Triticum vulgare L. and corn (Zea mays L. production, as examples. The objective of this study was to determine the relationship between agricultural systems and climatic change by using the Ricardian Method. Specific objectives were to evaluate and quantify the relationship of climatic variables (precipitation and temperature with economic variables under several realities of farms, to simulate the impact of scenarios of climatic change, to propose general orientations of adaptation and to evaluate the Ricardian Method with Chilean data. Economic and productive information from farmers belonging to Technological Transfer Groups (GTT of the Agricultural Research Institute (INIA was collected. The Ricardian Method explained 37.6% of land value variation. The highest values were in areas with moderate temperatures and precipitation. Temperature had a lower relationship to land value than precipitation. Under specific conditions (type of producer, irrigation, extension were detected behaviors that require further analysis. Upon simulating change of temperature and precipitation, the negative impacts on land value tended to be of lower magnitude than in other warmer regions. A tendency was observed for increased temperature to be beneficial, and a neutral to positive effect with less precipitation. The outputs could initially guide specific strategies of adaptation and mitigationEl cambio climático afectará los rendimientos y manejo de los cultivos agrícolas. Se estima que al año 2050 la temperatura media aumentaría 1,5 ºC, y al 2100 entre 1,0 y 3,5 ºC. En Chile existen pocos estudios sobre el tema

  18. Udder Morphological Traits and Milk Yield of Chilota and Suffolk Down Sheep Breeds Morfología Mamaria y Producción Lechera de las Razas Ovinas Chilota y Suffolk Down

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    María E Martínez

    2011-03-01

    Full Text Available Morphological traits of the whole udder (circumference, width, height, and length, teats (angle, length and width, udder cistern (teat opening and cistern height, and milk yield were measured over one lactation in a total of 18 ewes (nine Chilota and nine Suffolk Down sheep belonging to the same experimental flock (Centro Experimental INIA Butalcura, Chiloé, Chile. Analysis of variance showed significant effects for breed, sheep within breed, and stage of lactation. Chilota ewes evidenced greater udder circumference (433 mm, udder length (86 mm, teat opening (96º, and milk yield (947 mL d-1 than Suffolk Down ewes (410 mm, 77 mm, 93º, and 559 mL d-1, respectively. Udder circumference, udder width, udder length, cistern height and teat size significantly decreased throughout lactation. Repeatabilities of all traits were between 0.17 and 0.60; the highest repeatability was for udder circumference. This trait is easy to measure and had a discriminatory value for breed differentiation. In conclusion, Chilota sheep had a dairy aptitude greater than Suffolk Down sheep and similar to Spanish and Mediterranean dairy sheep breeds at beginning of their breeding programs.Los caracteres morfológicos de la ubre (perímetro, anchura, longitud y altura, de los pezones (ángulo de inclinación, longitud y anchura, de las cisternas mamarias (apertura de los pezones y altura de la cisterna y la producción lechera fueron medidos a lo largo de una lactación en un total de 18 ovejas del mismo rebaño (Centro Experimental INIA Butalcura, Chiloé, Chile, nueve de raza Chilota y nueve de raza Suffolk Down. El análisis de varianza mostró efectos significativos de la raza, la oveja dentro de la raza y del estado de lactación sobre las variables estudiadas. La raza Chilota evidenció un mayor perímetro de la ubre (433 mm, longitud de la ubre (86 mm, abertura de los pezones (96º, y producción lechera (947 mL d-1 que la Suffolk Down ( 410 mm, 77 mm, 93º, y 559 m

  19. Effect of cover crops management in aggregate stability of a vineyard in Central Spain.

    Science.gov (United States)

    Ruiz-Colmenero, Marta; Bienes, Ramon; Marques, Maria-Jose

    2010-05-01

    aggregates from treatments with cover crops had more organic carbon than the aggregates from traditional tillage treatment (Brachypodium distachyon 26.35, Secale cereale 18.83 and traditional tillage 17.04 g Kg-1). Lastly, the oxidable soil organic matter was also analyzed (Walkley-Black, 1934) and these results also indicated an increase in cover crop treatments, especially after the second year of treatment when the percentage of oxidable organic matter in the treatments with vegetable covers is approximately 1.5 times higher than this content in tillage treatment (1.015 %). The results support the conclusion that treatments with cover crops increased or at least maintained the stability of aggregates which is linked to the organic matter in the aggregates, on the contrary, the traditional tillage treatment showed less stable aggregates along the time. Keywords: aggregates stability, LOI, organic matter, vineyard, vegetable cover Aknowledgements: Projects FP06-DR3 IMIDRA, RTA2007-0086 INIA. Predoctoral INIA. Bodegas-Viñedos Gosálbez-Ortí.

  20. The influence of wine-distillery waste compost on nitrogen and phosphorus dynamics and uptake by a melon crop in a shallow calcareous soil

    Science.gov (United States)

    Requejo, M. I.; Villena, R.; Ribas, F.; Castellanos, M. T.; Cabello, M. J.; Arce, A.; Cartagena, M. C.

    2012-04-01

    In Mediterranean countries, the large quantity of organic wastes generated by the winery industry constitutes a serious environmental concern, due to its low pH and high content of phenolic compounds. This is accompanied by a seasonal production that makes their management difficult. Winery wastes are characterized by high organic matter contents, low electrical conductivity values and notable contents in macronutrients, so their use as organic amendments is a good management option for improving soil fertility. However, a composting treatment is necessary to convert these organic wastes into more stable, hygienic and humic-rich materials. The aim of this work was to evaluate the effects of the application of exhausted grape marc compost (composed of dealcoholized pulp, skins and stems) as fertilizer in soil nitrogen and phosphorus availability and uptake by a melon crop (Cucumis melo L.). This experiment was carried out from May to September 2011 in Ciudad Real (Spain). This area was designated "vulnerable zone" by the "Nitrates Directive" 91/676/CEE. The soil was a shallow sandy-loam (Alfisol Xeralf Petrocalcic Palexeralfs) with a depth of 0.60 m and a discontinuous petrocalcic horizon between 0.60 and 0.70 m, slightly basic (pH 7.9), poor in organic matter (0.20%), rich in potassium (407 ppm) and with a medium level of phosphorus (19.4 ppm). The experiment had a randomised complete block design, with four treatments consisted of four compost doses: 0 (D0), 6.7 (D1), 13.3 (D2) and 20 T compost ha-1 (D3), in order to determine the optimum dose to ensure nutrient demand, maximizing yield and minimizing nutrient losses. Acknowledgements This project has been supported by INIA-RTA2010-00110-C03-01.

  1. Effect of irrigation and winery waste compost rates in nitrate leaching in vulnerable zones

    Science.gov (United States)

    Requejo, Maria Isabel; Castellanos, Maria Teresa; Villena, Raquel; Ribas, Francisco; Jesús Cabello, Maria; Arce, Augusto; Cartagena, Maria Carmen

    2013-04-01

    The winery industry is widespread in Spain (3,610,000 tonnes of wine in 2010 (FAO, 2010)), and generates wastes characterized by a high content of organic matter, a notable content in macronutrients and low heavy-metals. These organic wastes could be used for agricultural purposes after a correct stabilization process (e.g. composting).The addition of these organic wastes requires a correct management, especially on semiarid cropped areas of central Spain where environmental degradation of water supplies with high N loads is observed. An integrated optimization of both applied compost dose and amount of irrigation is important to ensure optimum yields and minimum nitrate leaching losses. The purpose of this work was to study the effect of the application of winery waste compost as fertilizer in a melon crop cultivated with different drip irrigation rates. The field experiment was carried out in Ciudad Real, designated "vulnerable zone" by the "Nitrates Directive" 91/676/CEE. Melon crop has been traditionally cultivated in this area with high inputs of water and fertilizers, but no antecedents of application of winery wastes are known. Beside the control treatment (D0), three doses of compost were applied: 6.7 (D1), 13.3 (D2) and 20 T/ha(D3).Irrigation treatments consisted of applying a 100% ETc and an excess irrigation of 120% ETc. The soil was a shallow sandy-loam (Petrocalcic Palexeralfs), with 0.6 m depth and a discontinuous petrocalcic horizon between 0.6 and 0.7 m. Drainage and nitrate concentration on the soil solution were measured weekly to determine N leached during the crop period. Crop yield was also followed by harvesting plots when a significant number of fruits were fully matured. A comparison between nitrate leached and crop production among different treatments and irrigation rates are presented. Acknowledgements: This project has been supported by INIA-RTA2010-00110-C03-01.

  2. On the temporal and spatial variation of ozone in Cyprus.

    Science.gov (United States)

    Kleanthous, Savvas; Vrekoussis, Mihalis; Mihalopoulos, Nikolaos; Kalabokas, Pavlos; Lelieveld, Jos

    2014-04-01

    More than sixteen years (1997-2013) of continuous ozone concentrations at the rural Agia Marina (EMEP, 532 ma.s.l.) station in Cyprus, together with a number of ancillary chemical and meteorological parameters have been analyzed on a multiannual, annual and diurnal basis. The observations reveal a) the presence of a prominent seasonality with maxima observed during summer (54±5 ppbv) and the minima in winter (39±3 ppbv) b) a relatively small diurnal variability with the noon levels (50±9 ppbv) being higher by ~4 pbbv compared to nighttime (46±9 ppbv) and c) a non-significant upward trend over the 16 years of 0.11±0.12 ppbv y(-1). To assess the spatial variability over Cyprus, simultaneous measurements in 2011-2012 have been performed at Inia, Stavrovouni and Cavo Greco, three remote marine monitoring sites located to the west, central and the east of the Island, respectively. Our results show that ambient ozone levels over Cyprus are mostly influenced by regional/transported ozone while the local precursor emissions play a minor role in ozone formation. On an annual basis a net ozone reduction of 1.5 and 1.0 ppbv occurs when the air masses originate from northerly and westerly directions, respectively, while this is 2.4 ppbv during southerly wind. This suggests continuous net ozone loss controlled by surface deposition and photochemical destruction, and highlights the importance of long-range transport in controlling ozone levels in Cyprus.

  3. Increasing nitrogen rates in rice and its effect on plant nutrient composition and nitrogen apparent recovery

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    Juan Hirzel

    2013-12-01

    Full Text Available Rice (Oryza sativa L. is one of the essential foods of the human diet; advances in agronomic crop management can improve productivity and profitability as well as reduce adverse environmental impacts. Nitrogen rates in Chile are generally based on crop yield without considering other agronomic factors. The objective of this experiment was to determine the effect of increasing N rates on plant nutrient composition and N apparent recovery in rice cultivated in five different locations in Chile. The five sites located in central Chile belong to one of the following soil orders: Inceptisol, Alfisol, and Vertisol; they were cropped in field conditions with 'Zafiro-INIA' rice fertilized with 0, 80, and 160 kg N ha-1. Whole-plant total DM, macronutrient composition, and N apparent recovery efficiency (NARE were determined at grain harvest. Results indicate that all evaluated parameters, with the exception of K concentration, were affected by the soil used. Nitrogen rates only affected total DM production and P, K, and Mg concentrations in plants. Phosphorus and K response decreased when N was added to some soils, which is associated with its chemical properties. Magnesium concentration exhibited an erratic effect, but it was not affected by the N rate in most soils. Nitrogen apparent recovery efficiency was not affected by the N rate and accounted for approximately 49% and 41% for 80 and 160 kg N ha-1, respectively. Macronutrient composition was 5.1-7.7 g N, 1.3-1.8 g P, 5.4-10.8 g K, 1.68-2.57 g Ca, and 0.81-1.45 g Mg kg-1 of total DM.

  4. Efecto de la adición de materia orgánica sobre la dinámica poblacional bacteriana del suelo en cultivos de papa y maíz

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    David García Ventocilla

    2012-02-01

    Full Text Available Se determinó el efecto de la fertilización orgánica sobre las poblaciones bacterianas del suelo en cultivos de papa y maíz durante la campaña agrícola 2008-2009 en terrenos de cuatro localidades del Valle del Mantaro: INIA Santa Ana (Huancayo, en la EEA El Mantaro (Jauja, Vista Alegre y Huayao (ambos en Chupaca. En estos lugares se instalaron parcelas experimentales de papa (Solanum tuberosum L. Var. Canchan y maíz (Zea maíz L. Var. Cusco mejorado bajo abonamiento orgánico (vacuno, ovino, cuy, fertilización química y sin fertilización alguna (testigo. Para dicho efecto se empleó las técnicas de la Electroforesis en Gel de Gradiente Desnaturalizante (DGGE con amplificación de la región 968 – 1401 del rDNA 16S. Los resultados obtenidos muestran que la variabilidad de las poblaciones bacterianas en los suelos está afectado directamente por el tipo de cultivo mas no por el tipo de fertilización ya que el efecto de este último resulta variable para cada zona experimental y cultivo encontrándose solo en la zona experimental de Chupaca - Maíz una segregación de los tratamientos con fertilización orgánica de los tratamientos químicos. También se ha encontrado que la variación de las comunidades microbianas no sufre variaciones significativas en los suelos con cultivos de maíz obteniéndose coeficientes de similaridad para todos los tratamientos por encima del 80% mientras que para los tratamientos en los cultivos de papa dicho coeficiente fue de tan solo del 60%.

  5. Inactivation of the olfactory marker protein (OMP) gene in river dolphins and other odontocete cetaceans.

    Science.gov (United States)

    Springer, Mark S; Gatesy, John

    2017-04-01

    Various toothed whales (Odontoceti) are unique among mammals in lacking olfactory bulbs as adults and are thought to be anosmic (lacking the olfactory sense). At the molecular level, toothed whales have high percentages of pseudogenic olfactory receptor genes, but species that have been investigated to date retain an intact copy of the olfactory marker protein gene (OMP), which is highly expressed in olfactory receptor neurons and may regulate the temporal resolution of olfactory responses. One hypothesis for the retention of intact OMP in diverse odontocete lineages is that this gene is pleiotropic with additional functions that are unrelated to olfaction. Recent expression studies provide some support for this hypothesis. Here, we report OMP sequences for representatives of all extant cetacean families and provide the first molecular evidence for inactivation of this gene in vertebrates. Specifically, OMP exhibits independent inactivating mutations in six different odontocete lineages: four river dolphin genera (Platanista, Lipotes, Pontoporia, Inia), sperm whale (Physeter), and harbor porpoise (Phocoena). These results suggest that the only essential role of OMP that is maintained by natural selection is in olfaction, although a non-olfactory role for OMP cannot be ruled out for lineages that retain an intact copy of this gene. Available genome sequences from cetaceans and close outgroups provide evidence of inactivating mutations in two additional genes (CNGA2, CNGA4), which imply further pseudogenization events in the olfactory cascade of odontocetes. Selection analyses demonstrate that evolutionary constraints on all three genes (OMP, CNGA2, CNGA4) have been greatly reduced in Odontoceti, but retain a signature of purifying selection on the stem Cetacea branch and in Mysticeti (baleen whales). This pattern is compatible with the 'echolocation-priority' hypothesis for the evolution of OMP, which posits that negative selection was maintained in the common

  6. EVALUACIÓN DE DENSIDADES DE SIEMBRA EN Solanum sessiliflorum dunal “COCONA” Y SU EFECTO EN EL RENDIMIENTO DE FRUTO

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    Rodrigo Gonzales Vega

    2012-12-01

    Full Text Available La cocona es una especie vegetal nativa de América Tropical, cuyos frutos maduros son ricos en hierro, vitamina B5 (Niacina, se utilizan en la preparación de jugos, refrescos, jarabes y ensaladas. El estudio se realizó en el Campo Experimental “El Dorado” de Estación Experimental San Roque, del INIA Loreto con el objetivo principal de determinar el mejor distanciamiento para establecer plantaciones comerciales de Solanum sessiliflorum dunal “Cocona”, en condiciones de suelos no inundables o de altura. Se evaluaron cuatro densidades de siembra 2,0m x 2,0m; 1,5m x 1,5m, 2,0m x 1,0 y 1,5m x 1,0m. Las variables evaluadas fueron el número de frutos, peso de frutos y rendimiento de frutos por cada tratamiento en estudio, el diseño experimental utilizado fue Bloques Completos al Azar (DBCA, con cuatro repeticiones, los datos fueron analizados mediante el Análisis de Variancia y Prueba Estadística de Tukey, usando el software SPSS, versión 10. De los resultados Obtenidos se determinó que la densidad 1,5m x 1,0m (6 666 plantas/ha, produjo el mejor rendimiento con 14 600 kg/ha de fruto, superando significativamente a los tratamientos 2m x 1,0, 1,5m x 1,5m y 2,0 x 2,0m, que alcanzaron rendimientos de fruto de 12020, 11 330 y 10 570 kg/ha, respectivamente. Se concluye que la densidad de siembra influye en el rendimiento de fruto, así a menor número de plantas, mayor número y peso de fruto por planta se obtiene un menor rendimiento de fruto/ha.

  7. Fluctuación diurna del contenido de vitamina C en hojas de Myrciaria dubia “camu camu”

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    Franz Correa Meléndez

    2013-12-01

    Full Text Available Myrciaria dubia “camu camu” es un frutal del trópico amazónico caracterizado por sus frutos con gran contenido de vitamina C, siendo considerado un producto importante del país. Sin embargo, hay pocos reportes sobre el metabolismo y transporte de vitamina C en esta especie. El objetivo de la investigación fue determinar la fluctuación diurna del contenido de vitamina C en hojas de M. dubia “camu camu”. Las hojas se colectaron de la colección de germoplasma de “camu camu” del INIA a las 2, 6, 10, 14, 18 y 22 horas; de cuatro plantas y en tres fechas diferentes. La vitamina C fue extraída de las hojas con un método estandarizado en el laboratorio y se cuantificó mediante espectrofotometría a 530nm, previa reacción con 2,6-diclorofenolindofenol. Los resultados muestran que el contenido de vitamina C de las hojas de “camu camu” fue en promedio 231±35 mg vitamina C/100g de hoja. Además, el contenido de vitamina C en las hojas del “camu camu” mostró fluctuación diurna, siendo menor a las 6 horas y mayor a las 2 y 14 horas del día. También, se registraron concentraciones intermedias en horas de menor o ausencia de radiación solar (235±17 y 237±35 mg vitamina C/100g de hoja a las 18 y 22 horas respectivamente. Se concluye que existe alto contenido de vitamina C en las hojas del “camu camu” y que este contenido presenta fluctuación diurna.

  8. Environmental assay on the effect of poultry manure application on soil organisms in agroecosystems.

    Science.gov (United States)

    Delgado, M; Rodríguez, C; Martín, J V; Miralles de Imperial, R; Alonso, F

    2012-02-01

    This paper reports the effects produced on the organisms of the soil (plants, invertebrates and microorganisms), after the application of two types of poultry manure (sawdust and straw bed) on an agricultural land. The test was made using a terrestrial microcosm, Multi-Species Soil System (MS3) developed in INIA. There was no difference in the germination for any of the three species of plants considered in the study. The biomass was increased in the wheat (Triticum aestivum) coming from ground treated with both kinds of poultry manure. Oilseed rape (Brasica rapa) was not affected and regarding vetch (Vicia sativa) only straw poultry manure showed significant difference. For length only Vicia sativa was affected showing a reduction when straw was exposed to poultry manure. When the effect on invertebrates was studied, we observed a reduction in the number of worms during the test, especially from the ground control (13.7%), higher than in the ground with sawdust poultry manure (6.7%), whereas in the ground with straw poultry manure, there was no reduction. The biomass was affected and at the end of the test it was observed that while the reduction of worms in the ground control was about 48%, the number of those that were in the ground with sawdust poultry manure or straw poultry manure decreased by 41% and 22% respectively. Finally, the effects on microorganisms showed that the enzymatic activities: dehydrogenase (DH) and phosphatase and basal respiration rate increased at the beginning of the test, and the differences were statistically significant compared with the values of the control group. During the test, all these parameters decreased (except DH activities) but they were always higher than in the ground control. This is why it is possible to deduce that the contribution of poultry manure caused an improvement in the conditions of fertilization and also for the soil.

  9. Effect of N-acetyl cysteine and glycine supplementation on growth performance, glutathione synthesis, anti-oxidative and immune ability of Nile tilapia, Oreochromis niloticus.

    Science.gov (United States)

    Xie, Shiwei; Zhou, Weiwen; Tian, Lixia; Niu, Jin; Liu, Yongjian

    2016-08-01

    An 8-week feeding trial was conducted to evaluate the effect of N-acetyl cysteine (NAC) and glycine supplementation on growth performance, glutathione (GSH) synthesis, anti-oxidative and immune ability of Nile tilapia, Oreochromis niloticus. Four practical diets were formulated, control, control +0.2% NAC, control +0.5% glycine, control +0.2% NAC +0.5% glycine. Each diet was randomly assigned to quadruplicate groups of 30 fish (approximately 9.5 g). The weight gain and specific growth rate were significantly increased with the supplementation of NAC and glycine. While they had no effect on feed efficiency feed intake and survival. Glutathion peroxidase (GPx) was increased by NAC and γ-glutamine cysteine synthase (γ-GCS) in plasma were increased by glycine. After the feeding trail, fish were challenged by Streptococcus iniae, fish fed the diet supplemented with NAC obtained significantly higher survival rate after 72 h challenge test. NAC also decreased malonaldehyde (MDA) in liver, increased glutathione S-transferase (GST) activity in plasma, up-regulated mRNA expression of Superoxide dismutase (SOD) and GPx in liver and headkidney. Dietary supplementation of glycine increased the anti-oxidative ability of tilapia through increase anti-oxidative enzyme activity (SOD, glutathione reductase, myeloperoxidase) and up-regulate anti-oxidative gene expression (SOD). Immune ability only enhanced by the supplementation of NAC through increased interleukin-1β (IL-1β) mRNA expression. These results clearly indicated that the supplementation of NAC and glycine can significantly improve the growth performance of tilapia, and NAC also enhance the anti-oxidative and immune capacity of tilapia, glycine could only enhance the anti-oxidative ability.

  10. Risk of wine-distillery waste compost application in vulnerable zones: nitrogen balance

    Science.gov (United States)

    Requejo, M. I.; Villena, R.; Ventas, L.; Ribas, F.; Castellanos, M. T.; Cabello, M. J.; Arce, A.; Cartagena, M. C.

    2012-04-01

    Nitrogen (N) is the nutrient with the greatest impact on yield of horticultural crops. It is extremely dynamic in soil and undergoes changes that include processes of gains, losses and transformations. The melon crop area at Ciudad Real adds the 29% of the national production in Spain. The common agronomic management is representative of semiarid cropped zones of Spain where environmental degradation of water supplies with high N loads is observed. The site of this work is located near of Mancha Occidental aquifer (U.H.04.04, 6.953 km2) and Campo de Montiel aquifer (U.H. 04.06, 3.192 km2) with high contamination problems. The efficient use of fertilizers and irrigation is especially important in these areas designated vulnerables to nitrate pollution from agricultural sources. The aim of this study was to assess N losses when applying exhausted grape marc compost to a melon crop as source of nutrients in a vulnerable area. The doses are often excessive because are normally based on the input of organic matter rather than on the potentially mineralizable nitrogen. This N is not only released during the growing season but also in the intercropping period. In this experiment a nitrogen balance was carried out with three different doses of compost: 0 (D0), 6.7 (D1), 13.3 (D2) and 20 T compost ha-1 (D3). The soil was a shallow sandy-loam (Alfisol Xeralf Petrocalcic Palexeralfs), with a depth of 0.6 m and a discontinuous petrocalcic horizon between 0.6 and 0.7 m. Nitrogen plant absorption and nitrate losses were measured weekly, controlling at the same time N mineralized in soil. Simultaneously, a mineralization experiment was carried out without crop (either in laboratory and field conditions) to compare it with the results obtained with melon crop. Acknowledgements This project has been supported by INIA-RTA2010-00110-C03-01.

  11. Inducción de la biosíntesis local de antocianinas en frutos de Myrciaria dubia mediante lesiones mecánicas

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    Daniel Motta-Santillán

    2015-01-01

    Full Text Available En las plantas las antocianinas cumplen roles fundamentales en sus interacciones con el medio ambiente, son una de las líneas de defensa contra los radicales libres, la radiación ultravioleta y el ataque de patógenos. Observaciones realizadas muestran que los frutos de M. dubia acumulan antocianinas alrededor de lesiones mecánicas probablemente causadas por insectos. Por tanto, hemos probado la hipótesis que produciendo lesiones mecánicas en frutos verdes de M. dubia se induce la biosíntesis local de antocianinas. De tres plantas en fructificación de la colección de germoplasma del INIA se seleccionaron aleatoriamente 40 frutos verdes (25 ± 5 mm de Ø y se causaron lesiones mecánicas con estilete o bisturí al 75% de ellos. Posteriormente, se registró el porcentaje de acumulación de antocianinas por área superficial del fruto a las 0, 24, 48 y 72 horas. Se observó acumulación de antocianinas en ~10% (24 horas, ~30% (48 horas y ~70% (72 horas del área superficial de los frutos. En conclusión, las lesiones mecánicas causadas en frutos de M. dubia inducen la biosíntesis local de antocianinas, probablemente como un mecanismo de defensa efectivo desarrollado por esta especie. La comprensión de los mecanismos moleculares implicados en esta respuesta fisiológica nos permitirá establecer las bases para la mejora genética de esta especie.

  12. Inducción de la biosíntesis local de antocianinas en frutos de Myrciaria dubia mediante lesiones mecánicas

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    Daniel Motta Santillán

    2014-12-01

    Full Text Available En las plantas las antocianinas cumplen roles fundamentales en sus interacciones con el medio ambiente, son una de las líneas de defensa contra los radicales libres, la radiación ultravioleta y el ataque de patógenos. Observaciones realizadas muestran que los frutos de M. dubia acumulan antocianinas alrededor de lesiones mecánicas probablemente causadas por insectos. Por tanto, hemos probado la hipótesis que produciendo lesiones mecánicas en frutos verdes de M. dubia se induce la biosíntesis local de antocianinas. De tres plantas en fructificación de la colección de germoplasma del INIA se seleccionaron aleatoriamente 40 frutos verdes (25 ± 5 mm de Ø y se causaron lesiones mecánicas con estilete o bisturí al 75% de ellos. Posteriormente, se registró el porcentaje de acumulación de antocianinas por área superficial del fruto a las 0, 24, 48 y 72 horas. Se observó acumulación de antocianinas en ~10% (24 horas, ~30% (48 horas y ~70% (72 horas del área superficial de los frutos. En conclusión, las lesiones mecánicas causadas en frutos de M. dubia inducen la biosíntesis local de antocianinas, probablemente como un mecanismo de defensa efectivo desarrollado por esta especie. La comprensión de los mecanismos moleculares implicados en esta respuesta fisiológica nos permitirá establecer las bases para la mejora genética de esta especie.

  13. Characterization of a 1-cysteine peroxiredoxin from big-belly seahorse (Hippocampus abdominalis); insights into host antioxidant defense, molecular profiling and its expressional response to septic conditions.

    Science.gov (United States)

    Godahewa, G I; Perera, N C N; Elvitigala, Don Anushka Sandaruwan; Jayasooriya, R G P T; Kim, Gi-Young; Lee, Jehee

    2016-10-01

    1-cysteine peroxiredoxin (Prx6) is an antioxidant enzyme that protects cells by detoxifying multiple peroxide species. This study aimed to describe molecular features, functional assessments and potential immune responses of Prx6 identified from the big-belly seahorse, Hippocampus abdominalis (HaPrx6). The complete ORF (666 bp) of HaPrx6 encodes a polypeptide (24 kDa) of 222 amino acids, and harbors a prominent peroxiredoxin super-family domain, a peroxidatic catalytic center, and a peroxidatic cysteine. The deduced amino acid sequence of HaPrx6 shares a relatively high amino acid sequence similarity and close evolutionary relationship with Oplegnathus fasciatus Prx6. The purified recombinant HaPrx6 protein (rHaPrx6) was shown to protect plasmid DNA in the Metal Catalyzed Oxidation (MCO) assay and, together with 1,4-Dithiothreitol (DTT), protected human leukemia THP-1 cells from extracellular H2O2-mediated cell death. In addition, quantitative real-time PCR revealed that HaPrx6 mRNA was constitutively expressed in 14 different tissues, with the highest expression observed in liver tissue. Inductive transcriptional responses were observed in liver and kidney tissues of fish after treating them with bacterial stimuli, including LPS, Edwardsiella tarda, and Streptococcus iniae. These results suggest that HaPrx6 may play an important role in the immune response of the big-belly seahorse against microbial infection. Collectively, these findings provide structural and functional insights into HaPrx6.

  14. Depletion of florfenicol amine in tilapia (Oreochromis sp.) maintained in a recirculating aquaculture system following Aquaflor(R)-medicated feed therapy (20 mg/kg BW/d for 10 days)

    Science.gov (United States)

    Gaikowski, Mark P.; Whitsel, Melissa K.; Charles, Shawn; Schleis, Susan M.; Crouch, Louis S.; Endris, Richard G.

    2013-01-01

    Aquaflor® [50% w w−1 florfenicol (FFC)], is approved for use in freshwater-reared warmwater finfish which include tilapia Oreochromis spp. in the United States to control mortality from Streptococcus iniae. The depletion of florfenicol amine (FFA), the marker residue of FFC, was evaluated after feeding FFC-medicated feed to deliver a nominal 20 mg FFC kg−1 BW d−1 dose (1.33× the label use of 15 mg FFC kg−1 BW d−1) to Nile tilapia O. niloticus and hybrid tilapia O. niloticus × O. aureus held in a recirculating aquaculture system (RAS) at production-scale holding densities. Florfenicol amine concentrations were determined in fillets taken from 10 fish before dosing and from 20 fish at nine time points after dosing (from 1 to 240 h post-dosing). Water samples were assayed for FFC before, during and after the dosing period. Parameters monitored included daily feed consumption and biofilter function (levels of ammonia, nitrite and nitrate). Mean fillet FFA concentration decreased from 13.77 μg g−1 at 1-h post dosing to 0.39 μg g−1 at 240-h post dosing. Water FFC concentration decreased from a maximum of 1400 ng mL−1 at 1 day post-dosing to 847 ng mL−1 at 240 h post-dosing. There were no adverse effects noted on fish, feed consumption or biofilter function associated with FFC-medicated feed administration to tilapia.

  15. Effect of different mulch materials on the soil dehydrogenase activity (DHA) in an organic pepper crop

    Science.gov (United States)

    Moreno, Marta M.; Peco, Jesús; Campos, Juan; Villena, Jaime; González, Sara; Moreno, Carmen

    2016-04-01

    The use biodegradable materials (biopolymers of different composition and papers) as an alternative to conventional mulches has increased considerably during the last years mainly for environmental reason. In order to assess the effect of these materials on the soil microbial activity during the season of a pepper crop organically grown in Central Spain, the soil dehydrogenase activity (DHA) was measured in laboratory. The mulch materials tested were: 1) black polyethylene (PE, 15 μm); black biopolymers (15 μm): 2) Mater-Bi® (corn starch based), 3) Sphere 4® (potato starch based), 4) Sphere 6® (potato starch based), 5) Bioflex® (polylactic acid based), 6) Ecovio® (polylactic acid based), 7) Mimgreen® (black paper, 85 g/m2). A randomized complete block design with four replications was adopted. The crop was drip irrigated following the water demand of each treatment. Soil samples (5-10 cm depth) under the different mulches were taken at different dates (at the beginning of the crop cycle and at different dates throughout the crop season). Additionally, samples of bare soil in a manual weeding and in an untreated control were taken. The results obtained show the negative effect of black PE on the DHA activity, mainly as result of the higher temperature reached under the mulch and the reduction in the gas interchange between the soil and the atmosphere. The values corresponding to the biodegradable materials were variable, although highlighting the low DHA activity observed under Bioflex®. In general, the uncovered treatments showed higher values than those reached under mulches, especially in the untreated control. Keywords: mulch, biodegradable, biopolymer, paper, dehydrogenase activity (DHA). Acknowledgements: the research was funded by Project RTA2011-00104-C04-03 from the INIA (Spanish Ministry of Economy and Competitiveness).

  16. Efeito de diferentes níveis de fósforo em solução nutritiva e no solo no comportamento de cultivares de trigo Effect of different levels of phosphorus in nutrient solution and in the soil on the responses of wheat cultivars

    Directory of Open Access Journals (Sweden)

    Carlos Eduardo de Oliveira Camargo

    1984-01-01

    Full Text Available Foram estudados dez cultivares de trigo quanto à sua eficiência na utilização de fósforo em solução nutritiva contendo quatro níveis de P (0; 3,875; 7,75 e 15,5mg/litro, mantendo-se a temperatura constante de 25 °C ± 1 °C e o pH das soluções igual a 4,0 por um período de quinze dias. A eficiência da utilização de fósforo para cada cultivar em cada uma das concentrações desse elemento foi expressa pela quantidade de matéria seca da parte aérea e pela proporção entre a quantidade de matéria seca da parte aérea e a quantidade de fósforo nela presente. Os cultivares IAC-5, IAS-20, BH-1146 e IAC-17 foram eficientes na utilização de fósforo em soluções contendo 3,875mg/litro de P; IAC-18, IAC-15 e IAC-13 apresentaram-se como moderadamente eficientes, e Siete Cerros, Alondra-S-46 e INIA-66, como ineficientes. Os cultivares IAC-5, IAC-18 e INIA-66 responderam aumentando suas produções de matéria seca da parte aérea, à medida que o teor de P das soluções se elevou de 0 a 15,5mg/litro; IAS-20, IAC-13, Siete Cerros e Alondra-S-46 apresentaram moderada resposta e BH-1146, IAC-17 e IAC-15 não responderam. Os cultivares IAC-5 e IAC-17 foram estudados em vasos contendo solo nos quais foram empregadas três doses de adubação com superfosfato simples (0, 30 e 60kg/ha de P2O5 combinadas com três doses de calcário dolomítico (0, 4 e 8t/ha. Os dois cultivares responderam à adubação com P2O5 para os três níveis de calcário em relação à produção de matéria seca da parte aérea aos trinta dias após a germinação, porém o IAC-5 apresentou maiores respostas em relação ao IAC-17. Considerando fixo um mesmo nível de P2O5 verificou-se que os dois cultivares responderam à aplicação de calcário até a dosagem de 4t/ha. Os dois cultivares aumentaram a produção de grãos por planta quando foram aplicadas doses crescentes de P2O5, mantendo-se constante a dose de calcário de 4t/ha, porém as grandes respostas

  17. A teleostan homolog of catalase from black rockfish (Sebastes schlegelii): insights into functional roles in host antioxidant defense and expressional responses to septic conditions.

    Science.gov (United States)

    Elvitigala, Don Anushka Sandaruwan; Priyathilaka, Thanthrige Thiunuwan; Whang, Ilson; Nam, Bo-Hye; Lee, Jehee

    2015-05-01

    Antioxidative defense renders a significant protection against environmental stress in organisms and maintains the correct redox balance in cells, thereby supporting proper immune function. Catalase is an indispensable antioxidant in organisms that detoxifies hydrogen peroxides produced in cellular environments. In this study, we sought to molecularly characterize a homolog of catalase (RfCat), identified from black rockfish (Sebastes schlegelii). RfCat consists of a 1581 bp coding region for a protein of 527 amino acids, with a predicted molecular weight of 60 kD. The protein sequence of RfCat harbored similar domain architecture to known catalases, containing a proximal active site signature and proximal heme ligand signature, and further sharing prominent homology with its teleostan counterparts. As affirmed by multiple sequence alignments, most of the functionally important residues were well conserved in RfCat. Furthermore, our phylogenetic analysis indicates its common vertebrate ancestral origin and a close evolutionary relationship with teleostan catalases. Recombinantly expressed RfCat demonstrated prominent peroxidase activity that varied with different substrate and protein concentrations, and protected against DNA damage. RfCat mRNA was ubiquitously expressed among different tissues examined, as detected by qPCR. In addition, RfCat mRNA expression was modulated in response to pathogenic stress elicited by Streptococcus iniae and poly I:C in blood and spleen tissues. Collectively, our findings indicate that RfCat may play an indispensable role in host response to oxidative stress and maintain a correct redox balance after a pathogen invasion.

  18. Development and evaluation of a real-time fluorogenic loop-mediated isothermal amplification assay integrated on a microfluidic disc chip (on-chip LAMP) for rapid and simultaneous detection of ten pathogenic bacteria in aquatic animals.

    Science.gov (United States)

    Zhou, Qian-Jin; Wang, Lei; Chen, Jiong; Wang, Rui-Na; Shi, Yu-Hong; Li, Chang-Hong; Zhang, De-Min; Yan, Xiao-Jun; Zhang, Yan-Jun

    2014-09-01

    Rapid, low-cost, and user-friendly strategies are urgently needed for early disease diagnosis and timely treatment, particularly for on-site screening of pathogens in aquaculture. In this study, we successfully developed a real-time fluorogenic loop-mediated isothermal amplification assay integrated on a microfluidic disc chip (on-chip LAMP), which was capable of simultaneously detecting 10 pathogenic bacteria in aquatic animals, i.e., Nocardia seriolae, Pseudomonas putida, Streptococcus iniae, Vibrio alginolyticus, Vibrio anguillarum, Vibrio fluvialis, Vibrio harveyi, Vibrio parahaemolyticus, Vibrio rotiferianus, and Vibrio vulnificus. The assay provided a nearly-automated approach, with only a single pipetting step per chip for sample dispensing. This technique could achieve limits of detection (LOD) ranging from 0.40 to 6.42pg per 1.414μL reaction in less than 30 min. The robust reproducibility was demonstrated by a little variation among duplications for each bacterium with the coefficient of variation (CV) for time to positive (Tp) value less than 0.10. The clinical sensitivity and specificity of this on-chip LAMP assay in detecting field samples were 96.2% and 93.8% by comparison with conventional microbiological methods. Compared with other well-known techniques, on-chip LAMP assay provides low sample and reagent consumption, ease-of-use, accelerated analysis, multiple bacteria and on-site detection, and high reproducibility, indicating that such a technique would be applicable for on-site detection and routine monitoring of multiple pathogens in aquaculture.

  19. Effect of the time of application of phosphorus fertilizer on yield and quality parameters of melon crop amended with winery waste compost.

    Science.gov (United States)

    Requejo Mariscal, María Isabel; Cartagena, María Carmen; Villena Gordo, Raquel; Arce Martínez, Augusto; Ribas Elcorobarrutia, Francisco; Jesús Cabello Cabello, María; Castellanos Serrano, María Teresa

    2016-04-01

    In Spain, drip irrigation systems are widely used for horticultural crop production. In drip irrigation systems, emitter clogging has been identified as one of the most important concerns. Clogging is closely related to the quality of the irrigation water and the structure of the emitter flow path, and occurs as a result of multiple physical, biological and chemical factors. So, the use of acid fertilizers (e.g. phosphoric acid) in these systems is common to avoid the emitter clogging. Moreover, in this country the use of exhausted grape marc compost as source of nutrients and organic matter has been identified as a good management option of soil fertility, especially in grape-growing areas with a large generation of wastes from the wine and distillery industries. The purpose of this work was to study the effect of the time of application of phosphorus fertilizer with fertirrigation in a melon crop amended with winery waste compost on yield and quality parameters. During two years, the melon crop was grown under field conditions and beside the control treatment, three doses of compost were applied: 6.7, 13.3 and 20.0 t ha-1. All the compost treatments received 120 kg ha-1 of phosphorus fertilizer (phosphoric acid) for the season varying the time of application: The first year phosphorus application started after male and female flowering, and the second year the application started before flowering. Yield and quality parameters were evaluated to assess the suitability of these practices. Acknowledgements: This project has been supported by INIA-RTA2010-00110-C03. Keywords: Phosphorus fertilizer, exhausted grape marc compost, melon crop, yield and quality parameters.

  20. CLONACIÓN Y FILOGENIA MOLECULAR DE UN SEGMENTO DEL GEN CODANTE DE LA ACTINA DE MYRCIARIA DUBIA “CAMU-CAMU”: UN CANDIDATO PARA GEN DE REFERENCIA

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    Juan Carlos Castro Gómez

    2012-12-01

    Full Text Available Myrciaria dubia “camu-camu” es un frutal amazónico caracterizado por su amplia variación de vitamina C. Pero los estudios genético moleculares que puedan explicar esta variación son limitados. Por ello nuestro objetivo fue realizar la clonación y filogenia molecular de un segmento del gen codante de la actina de M. dubia. Las muestras fueron obtenidas de la colección de germoplasma del INIA. Luego, el ARN fue purificado y mediante RT-PCR con cebadores degenerados se amplificó un segmento del gen. En base a la secuencia obtenida se diseñaron cebadores específicos para PCR en tiempo real. Los resultados muestran que se ha aislado, clonado y secuenciado un segmento del gen codante de actina de M. dubia y detectado su expresión en hojas, pulpa y cáscara de M. dubia. Así, con el soporte de herramientas bioinformáticas y uso de técnicas de biología molecular hemos aislado, clonado y secuenciado un segmento del gen codante de la actina de M. dubia. Asimismo, los análisis realizados muestran que el gen se expresa y presenta niveles similares de expresión en hojas, pulpa y cáscara de M. dubia. Sin embargo, es necesario realizar más experimentos a fin de verificar su estabilidad de expresión.

  1. Las arañas en agroecosistemas: bioindicadores terrestres de calidad ambiental

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    Miguel Simó

    2011-12-01

    Full Text Available Las arañas son predadores generalistas en ecosistemas terrestres, ampliamente utilizadas en estudios de biodiversidad. En el último siglo, el paisaje de Uruguay se transformó en un mosaico de áreas formadas por ambientes naturales y agroecosistemas. ¿Qué nivel de cambio se espera hallar en la comunidad de arañas si las áreas de estudio están próximas? ¿Qué porcentaje de especies nativas serán reemplazadas con la plantación? ¿Cómo afecta esto a la biodiversidad local? Se realizó un estudio en cuatro áreas vecinas en INIA Las Brujas, Canelones: un bosque ribereño, un bosque de “Espinillo”, una plantación de Eucalyptus globulus y una pradera artificial de Trifolium pratense y Avena sativa. Las arañas fueron capturadas con trampas de caída, trampas de tronco, segado, batido de follaje y colecta manual nocturna. Un total de 3.023 adultos fueron colectados. La mayor abundancia y riqueza de especies se observó en el bosque ribereño. A pesar de la proximidad de los sitios estudiados, se observó un alto reemplazo de especies, especialmente entre áreas naturales y agroecosistemas. Los protocolos de colecta utilizados podrían ser útiles para evaluar el impacto de agroecosistemas con diferentes tipos de manejos sobre la biodiversidad local y su aplicación en la certificación de calidad ambiental.

  2. Molecular cloning and characterization of c-type lysozyme gene in orange-spotted grouper, Epinephelus coioides.

    Science.gov (United States)

    Wei, Shina; Huang, Youhua; Cai, Jia; Huang, Xiaohong; Fu, Jing; Qin, Qiwei

    2012-08-01

    Lysozymes are key proteins of the host innate immune system against pathogen infection. In this study, a c-type lysozyme gene (Ec-lysC) was cloned and characterized from orange-spotted grouper, Epinephelus coioides. The full-length Ec-lysC cDNA is composed of 533 bp and encodes a polypeptide of 144-residue protein with 94% identity to lysC of Kelp grouper, Epinephelus bruneus. The genomic DNA of Ec-lysC consists of 4 exons and 3 introns, with a total length of 1897 bp. Amino acid sequence alignment showed that Ec-lysC possessed conserved catalytic residues (Glu50 and Asp67) and "GSTDYGIFQINS" motif. RT-PCR results showed that Ec-lysC transcript was most abundant in head kidney and less in muscle. The expression of Ec-lysC was differentially up-regulated in head kidney after stimulation with lipopolysaccharide (LPS), Vibrio alginolyticus and Singapore grouper iridovirus (SGIV). Subcellular localization analysis revealed that Ec-lysC was distributed predominantly in the cytoplasm. The recombinant Ec-lysC (rEc-lysC) had lytic activities against Gram-positive bacteria Micrococcus lysodeikticus, Staphylococcus aureus, Streptococcus iniae and Gram-negative bacteria V. alginolyticus. The lysozyme acted on M. lysodeikticus cell walls as shown by scanning electron microscopy (SEM). Furthermore, overexpression of Ec-lysC in grouper cells delayed the occurrence of CPE induced by SGIV and inhibited the viral gene transcription significantly. Taken together, Ec-lysC might play an important role in grouper innate immune responses to invasion of bacterial and viral pathogens. C-type lysozyme gene from E. coioides (Ec-lysC) was identified and characterized.

  3. Rare serotype occurrence and PFGE genotypic diversity of Streptococcus agalactiae isolated from tilapia in China.

    Science.gov (United States)

    Li, Liping; Wang, Rui; Liang, Wanwen; Gan, Xi; Huang, Ting; Huang, Yan; Li, Jian; Shi, Yunliang; Chen, Ming; Luo, Honglin

    2013-12-27

    Previously, we reported 10 PEGE types of 85 tilapia Streptococcus agalactiae (GBS), which shifted from Streptococcus iniae in China, by using PEGE method. Presently, larger and more representative tilapia GBS were isolated, for the first time in China, to characterize their serotypes and genetic diversities more precisely than had done before. 168 GBS strains were distributed in five provinces of China, in which Guangdong, Guangxi and Hainan were the major ones, holding 36.9% (62/168), 37.5% (63/168) and 19.6% (33/168), respectively. Serotypes, Ia, Ib and III, were observed in these strains and the most predominant one was Ia (95.2%), which mainly distributed in Guangdong, Guangxi and Hainan. Ia initially occurred in 2009, it shoot up to 32.1% in 2010, but decreased to 16.1% in 2011 before went up to 45.2% in 2012. Ib sporadically occurred during 2007-2011, III only occurred in 2012. 14 different PFGE types, including 4 new types (N, O, P and Q), were observed, in which B, D, F and G were the predominant types, holding 83.9% (141/168) of the total GBS strains. Ia corresponded to 11 PFGE types (A-H, N-P), in which type D predominated (51%). Ib represented 3 genotypes (I, J and Q) and III harbored only 2 genotypes (N and F). Type N and F synchronously presented in Ia and III. In summary, the genetic diversity of tilapia GBS varied by serotypes and changed with geographical locations and years. Although Ia still predominated, new rare serotype III already occurred in China.

  4. Streptococcus hongkongensis sp. nov., isolated from a patient with an infected puncture wound and from a marine flatfish.

    Science.gov (United States)

    Lau, Susanna K P; Curreem, Shirly O T; Lin, Cherry C N; Fung, Ami M Y; Yuen, Kwok-Yung; Woo, Patrick C Y

    2013-07-01

    A bacterium, HKU30(T), was isolated from the infected tissue of a patient with wound infection after puncture by a fish fin. Cells are facultative anaerobic, non-spore-forming, non-motile, Gram-positive cocci arranged in chains. Colonies were non-haemolytic. The strain was catalase, oxidase, urease and Voges-Proskauer test negative. It reacted with Lancefield's group G antisera and was resistant to optochin. It grew on bile aesculin agar and in 5 % NaCl. It was unidentified by three commercial identification systems. 16S rRNA gene sequence analysis indicated that the bacterium shared 98.2, 97.7, 97.4 and 97.1 % nucleotide identities with Streptococcus iniae, Streptococcus pseudoporcinus, Streptococcus parauberis and Streptococcus uberis, respectively. The DNA G+C content was 35.6 ± 0.9 mol% (mean ± sd). In view of the occupational exposure of the patient, an epidemiological study was performed to isolate the bacterium from marine fish. Two strains, with similar phenotypic and genotypic characteristics to those of HKU30(T), were isolated from a three-lined tongue sole (Cynoglossus abbreviatus) and an olive flounder (Paralichthys olivaceus) respectively. Phylogenetic analysis of four additional housekeeping genes, groEL, gyrB, sodA and rpoB, showed that the three isolates formed a distinct branch among known species of the genus Streptococcus, being most closely related to S. parauberis (CCUG 39954(T)). DNA-DNA hybridization demonstrated ≤ 53.8 % DNA relatedness between the three isolates and related species of the genus Streptococcus. A novel species, Streptococcus hongkongensis sp. nov., is proposed. The type strain is HKU30(T) ( = DSM 26014(T) = CECT 8154(T)).

  5. Embryogenesis in Oak species. A review

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    Aranzazu Gomez-Garay

    2014-08-01

    Full Text Available Aim of study: A review on the propagation methods of four Quercus species, namely Q. suber, Q. robur, Q. ilex and Q. canariensis, through somatic embryogenesis and anther embryogenesis are presented.Area of study: The study comprises both Mediterranean and Atlantic oak species located in Spain.Material and Methods: Somatic embryogenesis was induced on immature zygotic embryos of diverse oak species, permitting the multiplication of half-sib families. Induction of haploid embryos and doubled haploids was assayed in both Q. suber and Q. ilex by temperature stress treatments of anthers containing late vacuolated microspores. The haploid origin of the anther embryos has been evaluated by quantitative nuclear DNA analysis through flow cytometry and by DNA microsatellite markers. Genetic transformation of cork oak has also been performed by means of Agrobacterium tumefaciens vectors. Proteomic analysis has been conducted to screen the diverse protein profiles followed by in vitro derived embryos during their development.Research highlights: Successful plant regeneration from both somatic and haploid embryos has been achieved. In the particular case of cork oak, doubled-haploid plants were obtained. Plantlets regenerated from selected parent trees through somatic embryogenesis were acclimated in the greenhouse and in the nursery, and were planted in an experimental plot in the field. Preliminary evaluation of the cork quality of the plants showed a good heritability correlation with the parent trees. This article revises the work of and is dedicated to Dr. M.A. Bueno, who devoted much of her professional life to the research on Biotechnology and Genetics of forest species, leading the Laboratory of Forest Biotechnology at the Spanish Institute of Agronomic Research (INIA.Key words: anther embryogenesis; microspore; pollen; Quercus canariensis; Quercus ilex; Quercus robur; Quercus suber; somatic embryogenesis. 

  6. Identification of Bacillus strains for biological control of catfish pathogens.

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    Chao Ran

    Full Text Available Bacillus strains isolated from soil or channel catfish intestine were screened for their antagonism against Edwardsiella ictaluri and Aeromonas hydrophila, the causative agents of enteric septicemia of catfish (ESC and motile aeromonad septicaemia (MAS, respectively. Twenty one strains were selected and their antagonistic activity against other aquatic pathogens was also tested. Each of the top 21 strains expressed antagonistic activity against multiple aquatic bacterial pathogens including Edwardsiella tarda, Streptococcus iniae, Yersinia ruckeri, Flavobacterium columnare, and/or the oomycete Saprolegnia ferax. Survival of the 21 Bacillus strains in the intestine of catfish was determined as Bacillus CFU/g of intestinal tissue of catfish after feeding Bacillus spore-supplemented feed for seven days followed by normal feed for three days. Five Bacillus strains that showed good antimicrobial activity and intestinal survival were incorporated into feed in spore form at a dose of 8×10(7 CFU/g and fed to channel catfish for 14 days before they were challenged by E. ictaluri in replicate. Two Bacillus subtilis strains conferred significant benefit in reducing catfish mortality (P<0.05. A similar challenge experiment conducted in Vietnam with four of the five Bacillus strains also showed protective effects against E. ictaluri in striped catfish. Safety of the four strains exhibiting the strongest biological control in vivo was also investigated in terms of whether the strains contain plasmids or express resistance to clinically important antibiotics. The Bacillus strains identified from this study have good potential to mediate disease control as probiotic feed additives for catfish aquaculture.

  7. Nitrogen balance as a tool to assess nitrogen mineralized from winery wastes under different irrigation strategies

    Science.gov (United States)

    Requejo, Maria Isabel; Castellanos, Maria Teresa; Villena, Raquel; Ribas, Francisco; Jesús Cabello, Maria; Arce, Augusto; Cartagena, Maria Carmen

    2013-04-01

    Grape marc is a by-product coming from the winery industry, composed of skins, seeds and stalks generated during the crushing process. In Spain, large quantities of wine are produced every year (3,610,000 tonnes in 2010 (FAO, 2010)) with the consequent waste generation. With an adequate composting treatment, this waste can be applied to soils as a source of nutrients and organic matter. Compost N forms added to soil are mostly organic N forms, so organic N can be mineralized during the crop period and thus be taken up by the plants, immobilised, or leached. Compost N mineralization depends on factors such as compost C/N ratio but also on climate conditions. Estimation of N mineralization is necessary to optimise crop yield and minimize the risk of N losses to the environment, especially in zones vulnerable to nitrate pollution. The aim of this work was to assess mineralized N during the crop season when applying grape marc compost as fertilizer in a melon crop cultivated under different drip irrigation rates. A nitrogen balance in field conditions was carried out with three different doses of compost: 0 (D0), 6.7 (D1), 13.3 (D2) and 20 T/ha (D3); and two irrigation rates (100% ETc and 120% ETc). The field experiment was carried out in Ciudad Real, designated "vulnerable zone" by the "Nitrates Directive" 91/676/CEE. The soil was a shallow sandy-loam (Petrocalcic Palexeralfs), with 0.6 depth and a discontinuous petrocalcic horizon between 0.6 and 0.7 m. Nitrogen plant uptake and nitrate losses were measured weekly; mineral N in soil was determined before compost addition and at the end of the crop cycle. An estimation of soil mineralized N during the crop season using nitrogen balance is presented. Results are compared with data obtained in laboratory conditions. Acknowledgements: This project has been supported by INIA-RTA2010-00110-C03-01.

  8. Molecular, morphological and pathogenic characterization of six strains of Metarhizium spp. (Deuteromycotina: Hyphomycetes for the control of Aegorhinus superciliosus (Coleoptera: Curculionidae

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    María Sepúlveda

    2016-03-01

    Full Text Available Aegorhinus superciliosus is an important pest on blueberry (Vaccinium corymbosum L. and other fruit trees. The use of entomopathogenic fungi as Metarhizium spp. has been evaluated for the control of this insect, but variability has been observed among different strains. The aim of this study was to characterize six promising strains of Metarhizium spp. for the control of A. superciliosus. The studied strains were QuM173c, Qu-M363, Qu-M171a, Qu-M156a, Qu-M421, and Qu-M430, all of which belonged to the Chilean Collection of Microbial Genetic Resources (ChCMGR of the Institute de Investigaciones Agropecuarias (INIA, Chile. Molecular characterization was made by sequencing the ITS region (Internal Transcribed Spacers, ITS-5.8S rDNA. The morphology of conidia was evaluated through scanning electron microscopy and radial colony growth was evaluated in potato dextrose agar (PDA, Sabouraud dextrose agar (SDA, agar enriched with larvae of Galleria mellonella (Lepidoptera: Pyralidae (GA, and agar enriched with adults of A. superciliosus (AA. Pathogenicity was studied based on mortality of adults of A. superciliosus inoculated with conidia. Sequencing of the ITS-5.8S rDNA region indicates that the strains belong to the clade of M. anisopliae var. anisopliae, except for Qu-M171a, which was identified as M. anisopliae var. lepidiotum. Conidia average length for the six strains was 5.09 pm and average conidia width was 1.92 pm. Radial colony growth differences were observed between strains (p < 0.01 and between different growth media (p < 0.01. The strains exhibited the highest colony growth in the GA medium, while in the AA medium they showed the lowest (p < 0.01. Pathogenicity tests show that Qu-M430 reached a 90% mortality rate (p < 0.01. Results show that there is variability between the studied strains, which is expressed in their morphology, molecular characteristics and pathogenicity towards A. superciliosus.

  9. Aplicación del coeficiente de inconsistencia como criterio de comparación entre mediciones Convencionales y Automáticas de lluvia diaria

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    Eva Colotti B.

    2003-01-01

    Full Text Available En Venezuela, los organismos que administran redes meteorológicas como el Ministerio del Ambiente y de los Recursos Naturales (MARN, la Fuerza Aérea Venezolana (FAV, la Armada Venezolana y Instituto Nacional de Investigaciones Agrícolas (INIA y Electrificación del Caroní (CVG-Edelca, entre otros, han iniciado la sustitución de los equipos convencionales de medición por mecanismos automáticos. Sin embargo, ésta sustitución instrumental sería procedente una vez que se hayan comparado simultáneamente las dos sucesiones de medidas, dado que, al evaluar la situación descrita, y haberse decidido eliminar el instrumental convencional, se genera un nuevo conjunto de datos que debería acoplarse a los registros antiguos medidos convencionalmente. Así, se propone un criterio para decidir si es necesario hacer ajustes a los nuevos datos automáticos con respecto a los convencionales o, equivalentemente, si es necesario posponer la eliminación del dispositivo convencional hasta que haya certeza en que las mediciones automáticas son fiables. El Error Cuadrático Medio (ECM fue seleccionado como un índice base en la toma de la decisión respectiva, habiéndose demostrado que ECM = V(X +V(X* - 2 C(X,X*, donde ECM = V(X-X* = varianza de las diferencias de mediciones automáticas y convencionales, V(X = varianza de la medición convencional, V(X* = varianza de la medición automática y C(X,X* = covarianza entre mediciones convencional y automática. Bajo la hipótesis de que E(X = E(X*, se concluye en que el cociente V(X-X* [V(X + V(X*], cuantifica el grado de inconsistencia entre los conjuntos muestrales provenientes de mediciones convencional y automática. Este cociente, denominado Coeficiente de Inconsistencia (acrónimo, CI, varía entre valores 0 (inconsistencia nula o consistencia máxima y 1 (inconsistencia máxima o consistencia nula y permitirá medir en el grado en que mediciones simultáneas bajo distintos criterios de medición son

  10. RESPONSES OF QUINOA (CHENOPODIUM QUINOA WILLD. TO TWO CONDITIONS OF IRRIGATION IN COAST

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    La Rosa, R.

    2008-01-01

    Full Text Available 13 Artículo original Biologist (Lima. Vol. 6, Nº1, ene-jun 2008, 13-21 RESPUESTAS DE LA QUINUA (CHENOPODIUM QUINOA WILLD. A DOS CONDICIONES DE RIEGO EN COSTA RESPONSES OF QUINOA (CHENOPODIUM QUINOA WILLD. TO TWO CONDITIONS OF IRRIGATION IN COAST Rafael La Rosa1, Yesenia Macabilca2, Augusto Mendoza3 & Ana Gutiérrez 3 1 Laboratorio de Ecofisiología Vegetal; 2 Laboratorio de Bioquímica y Biología Molecular3; Centro de Investigaciones Agroecológicas Oquendo. Facultad de Ciencias Naturales y Matemática. Universidad Nacional Federico Villarreal. Calle San Marcos 351, Pueblo Libre, Lima – Perú. Teléfono 2193600 anexo 8373. Correo electrónico: rafolarosa@yahoo.es ABSTRACT Responses of Chenopodium quinoa Willd “quinoa” under two coast conditions of irrigation and its effects in production and quality of proteins were evaluated. In the current research we used seeds of variety “huancayo”, from Experimental Station “Santa Ana” of INIA (Institute Nacional de Investigaciones Agropecuarias– Huancayo. Irrigation was for drip, obtaining two treatments, i irrigation with 3 m3 of water, weekly, and ii without irrigation, while evaluations were done. Soil humidity, transpiration, relative water content (RWC, anatomy of mesophyll of leaves, quantification of proteins and starch, acid phosphatase activity and amilolytic activity were evaluated. Soil humidity is significant different after a month of irrigation. Transpiration was related with wind strength; hence this is not a good way to measure drought stress. RWC was similar in both treatments, it means that decrease in soil water not affect water level in leaves. Mesophylls were very similar in both conditions. Dry matter in vegetative period show no significant differences, but there were a tendency to accumulate more assimilates in irrigated plants. Therefore there were no difference in photosynthetic activity, so seeds received same quantity of assimilates; this fact means seeds

  11. Zebrafish: an animal model for research in veterinary medicine.

    Science.gov (United States)

    Nowik, N; Podlasz, P; Jakimiuk, A; Kasica, N; Sienkiewicz, W; Kaleczyc, J

    2015-01-01

    The zebrafish (Danio rerio) has become known as an excellent model organism for studies of vertebrate biology, vertebrate genetics, embryonal development, diseases and drug screening. Nevertheless, there is still lack of detailed reports about usage of the zebrafish as a model in veterinary medicine. Comparing to other vertebrates, they can lay hundreds of eggs at weekly intervals, externally fertilized zebrafish embryos are accessible to observation and manipulation at all stages of their development, which makes possible to simplify the research techniques such as fate mapping, fluorescent tracer time-lapse lineage analysis and single cell transplantation. Although zebrafish are only 2.5 cm long, they are easy to maintain. Intraperitoneal and intracerebroventricular injections, blood sampling and measurement of food intake are possible to be carry out in adult zebrafish. Danio rerio is a useful animal model for neurobiology, developmental biology, drug research, virology, microbiology and genetics. A lot of diseases, for which the zebrafish is a perfect model organism, affect aquatic animals. For a part of them, like those caused by Mycobacterium marinum or Pseudoloma neutrophila, Danio rerio is a natural host, but the zebrafish is also susceptible to the most of fish diseases including Itch, Spring viraemia of carp and Infectious spleen and kidney necrosis. The zebrafish is commonly used in research of bacterial virulence. The zebrafish embryo allows for rapid, non-invasive and real time analysis of bacterial infections in a vertebrate host. Plenty of common pathogens can be examined using zebrafish model: Streptococcus iniae, Vibrio anguillarum or Listeria monocytogenes. The steps are taken to use the zebrafish also in fungal research, especially that dealing with Candida albicans and Cryptococcus neoformans. Although, the zebrafish is used commonly as an animal model to study diseases caused by external agents, it is also useful in studies of metabolic

  12. Impact of climate change on olive crop flowering at southern Spain

    Science.gov (United States)

    Gabaldón-Leal, Clara; Ruiz-Ramos, Margarita; de la Rosa, Raúl; León, Lorenzo; Rodríguez, Alfredo; Belaj, Angjelina; Lorite, Ignacio

    2016-04-01

    Andalusia region (Southern Spain) is the largest olive oil producer in the world with 40% of the total production (1.1millions tons; FAOSTAT, 2013). Predicting flowering dates of olive under future climate conditions has a critical importance as flowering is a critical stage for olive as heat and water stresses during this period have a significant impact on final olive oil yield. The aim of this study was to evaluate the olive flowering dates under future climate conditions. Climate data for the future period was obtained from the ENSEMBLES European Project (http://www.ensembles-eu.org/) with a bias correction in temperature and precipitation with regard with the SPAIN02 dataset (Herrera et al., 2012) (ENS-SP). Flowering of ten different olive cultivars were evaluated under current and forced (greenhouse) climate conditions, applying the bias in temperatures comparing the current period (1981-2010) with the future period (2071-2100) to the current conditions in Cordoba (Andalusia, Spain). These observations allowed obtaining a flowering approach which was applied to the whole Andalusia region. The results showed an average advance in flowering dates about 16 days at the end of the 21st century. With these results different areas within Andalusia region were classified based on the vulnerability caused by the lack of chilling hours accumulation (coastal areas) or by high temperatures during flowering (north and northeast regions). This study has been supported by the project RTA2014-00030-00-00 funded by INIA and FEDER 2014-2020 "Programa Operativo de Crecimiento Inteligente" and IFAPA project AGR6126 from Junta de Andalucía, Spain. FAOSTAT (2013) Food And Agriculture Organization Of The United Nations. Available at http://faostat3.fao.org/browse/Q/QD/E. Accessed 07 January 2016 Herrera S, Gutiérrez JM, Ancell R, Pons MR, Frías MD, Fernández J. 2012. Development and analysis of a 50-year high-resolution daily gridded precipitation dataset over Spain (Spain02

  13. Expressed sequence tags (ESTs) based identification of genes and expression analysis of leukocyte cell-derived chemotaxin-2 (LECT2) from Epinephelus bruneus.

    Science.gov (United States)

    Harikrishnan, Ramasamy; Kim, Ju-Sang; Kim, Man-Chul; Balasundaram, Chellam; Heo, Moon-Soo

    2012-01-01

    The kelp grouper, Epinephelus bruneus, is an economically important intensively cultured species in Southeast Asia. Despite the insatiable demand its large-scale production has been hindered by problems associated with water quality, nutrition, and diseases especially due to increased rearing density. It is generally accepted that in fish both innate and adaptive immune system provide protection from diseases. In the present study a cDNA library of Streptococcus iniae-challenged kelp grouper was constructed to identify the genes that reveal molecular mechanism, physiological functions, and gene expression in different tissues using expressed sequence tags (ESTs) and RT-PCR strategy. Of a total of 2170 ESTs examined 279 (12.9%) were identified as contig and 860 (39.6%) as singletons. A total of 190 important immune and enzyme related genes (16.7%) were identified in both contig and singletons. The key immune molecules identified comprise complement factors, chemotaxin, chemokine, Fas ligand, ferritins, hepcidin, lysozyme c, MHC, and TLR which are involved in the innate or adaptive immune system. Among the genes a full-length cDNA of leukocyte cell-derived chemotaxin-2 (EbLECT2) with 540 base pair (bp) was identified; it consists of a 5'-untranslated region (UTR) of 17 bp, a 3'-UTR of 76 bp, and a stop codon TAA in 3'-UTR. The EbLECT2 is an important molecule in the innate immunity. It is a multifunctional protein involved in cell growth, differentiation, and autoimmunity. The open reading frame (ORF) of the EbLECT2 encodes with 155 amino acid (aa) residues with a predicted molecular weight and isoelectric point (pI) of 17 kDa and 9, respectively. The close phylogenetic relationship of EbLECT2 shares the highest similarity with the already reported LECT2 from Epinephelus coioides (96%) and Epinephelus akaara (94%). EbLECT2 mRNA was expressed predominantly in liver, spleen, and kidney while the expression was moderate in gills, heart, and muscle in E. bruneus after

  14. ELEMENTOS ESTRATÉGICOS DE LA GESTIÓN DEL CONOCIMIENTO ORGANIZACIONAL PARA LA INNOVACIÓN. CASO: RED DE AGROMETEOROLOGÍA

    Directory of Open Access Journals (Sweden)

    Barlin Orlando Olivares

    2016-06-01

    Full Text Available RESUMEN Esta experiencia docente tiene como objetivo proponer un modelo de gestión del conocimiento organizacional para el programa de capacitación desarrollado por el departamento de agrometeorología del Instituto Nacional de Investigaciones Agrícolas (INIA del estado Anzoátegui, Venezuela. Se utilizó el protocolo de solución de problemas, derivado de la aplicación de ciclos de aprendizaje, denominado TADIR (Traducción, Análisis, Diseño, Implementación y Revisión. Se estableció la diversificación de las formas de promover las actividades, tareas, resultados, productos generados por el proyecto, antes, durante y posterior a su ejecución, de manera fácil y completa, así como la conciencia en la población sobre las bondades de la agrometeorológica y su importancia para el desarrollo agrícola del país. Los resultados de esta investigación pueden estar al servicio de la misma organización de manera directa e indirecta y de otras que quieran asumir el reto de contribuir al proceso de transformación que vive la nación. ABSTRACT This teaching experience seeks to propose an organizational knowledge management model for the training program developed by the Agrometeorology Department of the National Institute for Agricultural Research (INAI, in Spanish of the State of Anzoátegui, Venezuela. The problem solution protocol deriving from the application of learning cycles, TADIR (Translation, Analysis, Design, Implementation and Revision, was used. An array of different ways to promote activities, tasks, results, and products generated by the project was established before, during and after the execution in an easy and complete manner. The population was also made aware of the advantages of agrometeorology and its importance for the agricultural development of the country. The results of this research can be made directly or indirectly available to the same organization or any others who wish to take the challenge of contributing

  15. Yield, nutrient utilization and soil properties in a melon crop amended with wine-distillery waste compost

    Science.gov (United States)

    Requejo Mariscal, María Isabel; Villena Gordo, Raquel; Cartagena Causapé, María Carmen; Arce Martínez, Augusto; Ribas Elcorobarrutia, Francisco; Jesús Cabello Cabello, María; Castellanos Serrano, María Teresa

    2014-05-01

    suitability of this compost as organic amendment. Acknowledgements: This project has been supported by INIA-RTA2010-00110-C03-01

  16. Study of the degradation of mulch materials in vegetable crops for organic farming

    Science.gov (United States)

    María Moreno, Marta; Mancebo, Ignacio; Moreno, Carmen; Villena, Jaime; Meco, Ramón

    2014-05-01

    early but once they have fulfilled their functions, appearing as a good alternative to PE, especially in organic farming. Project INIA RTA2011-00104-C04-03. References: Kasirajan, S.; Ngouajio, M. 2012. Polyethylene and biodegradable mulches for agricultural applications: a review. Agron. Sustain. Dev. 32: 501-529. Martín-Closas, L.; Pelacho, A.M. 2011. Agronomic potential of biopolymer films. p. 277-299. In: Biopolymers. New materials for sustainable films and coating. John Wiley & Sons, New York. Moreno, M.M.; Moreno A. 2008. Effect of different biodegradable and polyethylene mulches on productivity and soil thermal and biological properties in a tomato crop. Sci. Hort. 116(3): 256-263.

  17. Depletion of florfenicol amine, marker residue of florfenicol, from the edible fillet of tilapia (Oreochromis niloticus x O. niloticus and O. niloticus x O. aureus) following florfenicol administration in feed

    Science.gov (United States)

    Gaikowski, M.P.; Mushtaq, M.; Cassidy, P.; Meinertz, J.R.; Schleis, S.M.; Sweeney, D.; Endris, R.G.

    2010-01-01

    Aquaflor??, a 50% feed premix containing the broad spectrum antibacterial agent florfenicol is available globally to control mortality associated with economically significant systemic bacterial diseases of fish. Florfenicol (FFC) is effective in controlling mortality associated with Streptococcus iniae in tilapia Oreochromis sp. when administered in medicated feed at a dose of 15 mg/kg bodyweight (BW)/d for 10 consecutive days. Our objective was to characterize the depletion of the FFC marker residue, florfenicol amine (FFA), from the edible tissue of market-weight Nile tilapia O. niloticus x O. niloticus and hybrid tilapia O. niloticus x O. aureus offered feed medicated with FFC at a nominal dose rate of 15 mg/kg BW/d for 12 days. Near market-weight tilapia were obtained from a commercial tilapia farm, distributed to 2 single pass (one for Nile tilapia and one for hybrid tilapia), flow-through systems and maintained at 27 ??C under a 15 h light:9 h dark photoperiod over a 41-d pre-dosing period. During the dosing period, tilapia were offered feed medicated with FFC at a concentration of 1.479 g/kg at 1% BW daily divided in three equal offerings. The initial 10-d dosing period was extended to 12 d because one tank did not consume > 75% of the feed offered during the first two dosing days. The total dose consumed by fish in each of the 2 tanks ranged from 147 to 167 mg/kg. Once during the pre-dose period and on days 1, 2, 4, 7, 14, 21, and 28 of the post-dose period, groups of fish were indiscriminately removed from each tank, measured for weight and length, scaled, filleted, and the skin-on fillets stored at performance liquid chromatography with UV detection. Florfenicol amine is rapidly eliminated from tilapia fillet after withdrawal from medication and depletion followed first-order kinetics with an estimated half-life of 2.32 d. The FFA tolerance limit, calculated as the 99th percentile of the potential residue level at 95% confidence, had depleted to less

  18. Molecular aspects, genomic arrangement and immune responsive mRNA expression profiles of two CXC chemokine receptor homologs (CXCR1 and CXCR2) from rock bream, Oplegnathus fasciatus.

    Science.gov (United States)

    Umasuthan, Navaneethaiyer; Wan, Qiang; Revathy, Kasthuri Saranya; Whang, Ilson; Noh, Jae Koo; Kim, Seokryel; Park, Myoung-Ae; Lee, Jehee

    2014-09-01

    that of OfCXCR1. In addition, the magnitude of the OfCXCR1 and OfCXCR2 transcripts in head kidney and spleen was differentially increased after the in vivo administration of immune stimulants, LPS and poly I:C and in the infection models injected with rock bream irido virus, Edwardsiella tarda and Streptococcus iniae. These lines of evidence suggest that these receptors may play an important role(s) in immune responsive signaling during pathogenesis of rock bream.

  19. Streptococcus agalactiae: HASTA AHORA EL ÚNICO Streptococcus PATÓGENO DE TILAPIAS CULTIVADAS EN COLOMBIA

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    AP Jiménez

    2007-01-01

    Full Text Available Las estreptococosis son un conjunto de enfermedades ocasionadas por un grupo de cocos Gram positivos con similares signologías que involucran distintos órganos en los individuos afectados. La identiÀcación precisa de cada uno de estos microorganismos no se logra de manera deÀnitiva por los métodos tradicionales microbiológicos, por lo que se debe acudir a otro tipo de metodologías como las técnicas de biología molecular. En 1999 se identiÀcó por primera vez en Colombia la estreptococosis en híbridos de tilapias de cultivo. La pos-terior secuenciación del ADN de distintos aislamientos obtenidos de varias regiones del país demostró un 98,8% de aÀnidad con el Streptococcus agalactiae. El presente estudio pretende deÀnir si hasta la fecha existe solo esta especie de Streptococcus en el país causan-do infección o enfermedad en tilapias de cultivo o, por el contrario, son varias las especies que intervienen en los cuadros infecciosos. Se evaluaron aislamientos de tejidos, de agua y fango de los sitios de cultivo, así como de lugares de expendio de tilapia roja (Oreochromissp., utilizando técnicas microbiológicas, inmunoperoxidasa indirecta (IPI y PCR, espe-cíÀcas para el aislamiento e identiÀcación del S. agalactiae. Los resultados del presente estudio demostraron que hasta la fecha en el país únicamente se ha identiÀcado la especie S. agalactiae causando infección o enfermedad en tilapias. No tenemos evidencia de que otros Streptococcus reportados internacionalmente como S. iniae y otros Gram positivos causen estreptococosis en Colombia.La tilapia parece ser el principal reservorio del S. agalactiae en el país, y el riesgo zoonótico, aunque existe, es mínimo si se toman las medidas apropia-das de bioseguridad.

  20. Molecular cloning and characterization of a new G-type lysozyme gene (Ec-lysG) in orange-spotted grouper, Epinephelus coioides.

    Science.gov (United States)

    Wei, Shina; Huang, Youhua; Huang, Xiaohong; Cai, Jia; Wei, Jingguang; Li, Pengfei; Ouyang, Zhengliang; Qin, Qiwei

    2014-10-01

    Lysozyme acts as an innate immunity molecule against pathogen infection. In this study, a new G-type lysozyme gene with a typical G-type lysozyme domain (designated as Ec-lysG) was cloned and characterized from the orange-spotted grouper, Epinephelus coioides. The full-length Ec-lysG cDNA contains 1419 bp and encodes a 256-residue protein containing a 25-residue signal peptide at the N-terminus. BLAST analysis reveals Ec-lysG shares 64% identity with Siniperca chuatsi, but 63% to another reported G-type lysozyme from orange-spotted grouper (OSG-lysG). The genomic DNA of Ec-lysG contains four exons and three introns, with a total length of 2062 bp. An amino acid sequence alignment showed that Ec-lysG shares the fundamental structural features of G-type lysozyme, including the catalytic residues, substrate binding sites, and soluble lytic transglycosylase domain. Quantitative PCR showed that Ec-lysG transcript is most abundant in the head kidney, and less abundant in the heart. The expression of Ec-lysG was differentially upregulated in the head kidney after stimulation with lipopolysaccharide, Vibrio alginolyticus, and Singapore grouper iridovirus (SGIV). A subcellular localization analysis showed that Ec-lysG is distributed predominantly in the cytoplasm. Recombinant Ec-lysG (rEc-lysG) has optimal activity at pH 7.5 and 35°C. rEc-lysG showed lytic activities against Gram-positive bacterium Streptococcus iniae, Staphylococcus aureus, and Micrococcus lysodeikticus, and the Gram-negative bacterium V. alginolyticus. Scanning electron microscopy (SEM) showed that rEc-lysG acts on M. lysodeikticus cell walls. The overexpression of Ec-lysG in grouper cells did not significantly delay the occurrence of the cytopathic effect (CPE) induced by SGIV, and did not inhibit viral gene transcription. In conclusion, Ec-lysG might be a potent antibacterial protein, with a role in innate immunity.

  1. Separation and Identification of Erwinia caratovora ssp . caratovora Pathogen of Coriandrum sativum L%芫荽软腐病病原菌分离与鉴定

    Institute of Scientific and Technical Information of China (English)

    张立微; 张景涛; 李小梅

    2015-01-01

    为有效防治芫荽软腐病,提高其产质量,通过对引起芫荽腐烂而绝产的病害的病原菌进行分离、纯化,对19个菌株进行17项生理生化指标测定。结果表明:根据伯杰氏细菌鉴定手册(第八版)以 Ecc为对照进行鉴定,确定E1(Ames24923)、E3(Ames24927)、E4(CORI107)、E7(CORI139)、E8(CORI140)、E10(CORI147)、E12(CORI289)、E13(CORI318)、E18(PI664510)为芫荽胡萝卜欧文氏杆菌属胡萝卜软腐欧文氏杆菌胡萝卜软腐病亚种(Erw inia carotovora ssp .carotovora ,Ecc)细菌,而其余供试菌株通过测定可确定为细菌,但属何种还需进一步鉴定。%In order to control the Erwinia caratovora ssp .caratovora of Coriandrum sativum L .,and improve the quality ,through the isolating and purificating of the pathogen which causes coriander decay ,19 strains were isolated and purified .The results showed that according to Bergey's Manual of Systematic Bacteriology (eighth edition) and 17 tests of physiology and biochemistry ,9 strainss were Erwiniacarotovora ssp .carotovora ,inclu‐ding E1(Ames24923) ,E3(Ames24927) ,E4(CORI107) ,E7(CORI139) ,E8(CORI140) ,E10(CORI147) ,E12 (CORI289) ,E13(CORI318) ,E18(PI664510) .Other strains should to be identified by other tools .

  2. Revista História da Educação, v. 14, n. 30, 2010

    Directory of Open Access Journals (Sweden)

    RHE Asphe

    2011-03-01

    Full Text Available

    É com enorme satisfação que a revista História da Educação publicada pela Associação Sul Rio-Grandense de História da Educação - Asphe - trás á público mais um número de seu periódico. Sempre com a missão de divulgar trabalhos de investigadores nacionais e internacionais de renome na área de história da educação a comissão editorial deste número tem a certeza que mais uma vez tem o prazer de apresentar textos de excepcional qualidade.

    Abre este número o trabalho da professora Carlota Josefina Malta Cardozo dos Reis Boto: Compêndios pedagógicos de augusto coelho (1850-1925: a arte de tornar ciência o ofício de ensinar, no qual com brilhantismo a autora analisa a obra pedagógica deste renomado autor.

    A seguir, a professora Beatriz T. Daudt Fischer com o artigo Revista do Ensino/RS e Maria de Lourdes Gastal: duas histórias em conexão, analisa a revista mais consagrada da historiografia sobre educação do Rio Grande do Sul: a Revista do Ensino e a história de vida de Maria de Lourdes Gastal.

    Os dois textos seguintes decorrem da participação das professoras Ana Chrystina Venâncio Mignot e Claudia Alves no 14° Encontro de Pesquisadores de História da Educação do Rio Grande do Sul, realizado em 2009 no qual as referidas professoras participaram como conferencistas respectivamente com os trabalhos: Sobre coisas de outros tempos: rastros biográficos nas crônicas de Cecília Meireles na “página de educação” e Educação, memória e identidade: dimensões imateriais da cultura material escolar.

    A consagrada pesquisadora em História da Educação Mirian Jorge Warde juntamente com Claudia Panizzolo nos brindam com o texto as fontes do método analítico de leitura de João Köpke (1896-1917. Trabalho de altíssima qualidade e deve constituir de referência na área.

    Os professores portugueses Maria João Mogarro Fernanda Gon

  3. Potassium and Phosphorus in Muscat Rosada Grape Yield in Elqui Valley Soil Fósforo y Potasio en la Producción de Vid Moscatel Rosada, en Suelo del Valle de Elqui

    Directory of Open Access Journals (Sweden)

    Carlos Sierra B

    2008-09-01

    Full Text Available The effects of P and K on grapevines (Vitis vinifera L. var. Muscat Rosada were evaluated four years, considering the same levels of N. The experiment was conducted at the Vicuña Experimental Station (30° S; 70°44´ W of the Instituto de Investigaciones Agropecuarias (INIA. The soil is alluvial antropic miscellaneous (Entisols. Three fertilization treatments were established: 1 N 160 + P2O5 200 + K2O 300; 2 N 160 + P2O5 0 + K2O 300; and 3 N 160 + P2O5 200 + K2O 0. At the beginning of the experiment, grape vines received all the phosphate fertilizers, 200 kg ha-1 of P2O5 as triple superphosphate in Treatments 1 and 3. Nitrogen was applied as urea for four years. The applications were initiated when the vine buds measured 20 cm. The K was applied to the soils of Treatments 1 and 2, at a dosage of 200 kg ha-1 K2O, using potassium sulphate. Between berry set and 30 d before harvest, 100 kg ha-1 K2O were applied as potassium sulphate by fertigation. Significant effects of the P fertilization were observed by the second year, with increased cluster numbers per plant. K also increased grape fruit yield by the third year. This response was obtained with less than 5 mg kg-1 of P available in the soil and less than 145 mg kg-1 of exchangeable K in the soil. The initially low content of available K and P suggests a high probability of response to the application of both elements.En el Campo Experimental del Instituto de Investigaciones Agropecuarias (INIA, ubicado en la localidad de Vicuña (30° S; 70°44´ O, se evaluó durante cuatro años el efecto de la aplicación de P y K, considerando un mismo nivel de aplicación de N en vid (Vitis vinifera L. var. Moscatel Rosada. El suelo corresponde al tipo misceláneo antrópico coluvial (Entisols. Se establecieron tres tratamientos de fertilización: 1 N 160 + P2O5 200 + K2O 300; 2 N 160 + P2O5 0 + K2O 300; y 3 N 160 + P2O5 200 + K(20 0. El parrón recibió toda la fertilización fosfatada aplicada al

  4. The Selection and Determinaton of the Pest-resistant Character in    Transgenic Regenerative Rice Plants and Their Descendances%水稻转基因再生植株及其后代抗虫性筛选测定研究

    Institute of Scientific and Technical Information of China (English)

    冉学忠; 王立敏; 孙伯铮; 陈方江; 刘春颖

    2001-01-01

    Using gene gun transformation system, we transfered CpTI (Cowpea Trypsin Inhibitor)gene to rice callus tissues;through anti-biotic selection.The transgenic regenerative rice plants were gotten. Indoor feeding corn borer(Ostrinia nubilalis) by these transgenic rice regenaration plants and their descendance’s tiller stem;The corn borer mortality and the worm length were compared to determinate transgenic regenerative rice’s pest resistance. From 1997 to 1999,5 indoor tests had been taken on three generations. 15 better pest resistance plants were selected from 121 plants in F0 generation, 7 strains 40 better single plants from 15 strains in F1 generation,and 37 better plants from 14 strains in F2 generation. We did molecular test(PCR) to 19 plants from the 37 better plants,and asserted that 5 material are homozygous plants, the other 14 material have segregation. All of these stated that the anti-pest gene in transgenic regenerative rice can express and is heritable%采用基因枪转化系统,将CpTI(豇豆胰蛋白酶抑制剂)抗虫基因转入水稻愈伤组织中,经过抗生素筛选得到了再生植株。我们用这些转基因水稻再生植株及其后代的分蘖茎段对玉米螟(Ostrinia nubilalis)进行室内喂养,通过比较虫体死亡率及虫体生长长度,测定转基因水稻再生植株的抗虫性。1997~1999年共进行3个世代5次的室内测定。从121株F0代转基因植株中选出抗虫性较好的植株15株。从F1代的15个株系中,又筛选出7个株系40个单株;在F2代的14个株系中共选出37株抗虫效果表现较好的植株。对37株中的19株进行了分子检测(PCR扩增),从中检测出5份材料为纯合体,14份材料有分离现象。研究结果说明:该室内喂养玉米螟并进行抗虫测定的方法是可行的;抗虫基因在转基因水稻再生植株中已经获得表达并且可以遗传

  5. Estudio macro-microscópico de la involución uterina postparto en alpacas - Macro and microscopic of uterine involution postpartum in alpacas

    Directory of Open Access Journals (Sweden)

    MVZ. Augusto Excelmes Loza. Puno-Perú 2005

    2006-07-01

    Full Text Available El presente estudio se llevo a cabo en el Laboratorio de Histología y Embriología de la Facultad de Medicina Veterinaria y Zootecnia de la Universidad Nacional del Altiplano; con alpacas provenientes de la Rural Alianza E.P.S. (4,140m.s.n.m, Centro Experimental Quinsachata-INIA (4,050 m.s.n.m y el Centro de Investigación y Producción La Raya (4,130 m.s.n.m, cuyo objetivo fue el estudio del proceso de la involución uterina post parto en la alpaca, desde el parto hasta los 30dpp. Para tal propósito se estudiaron 34 alpacas multíparas de 4 a 6 años de edad. Los resultados fueron: Macroscópicamente: la perdida de peso del útero fue de: 71.07% (385,25g, 25.54% (138.55g y 3.42% (18.6g al primer, segundo y tercer periodo de la involución uterina. La reducción de Peso del cuerno uterino derecho fue de: 55.26% (37.55g, 38.63% (26.25g y 6.11% (4.15g al primer segundo y tercer periodo respectivamente. La secuencia de la perdida de peso del cuerno uterino izquierdo fue de: 75.50% (159.5g, 19.58% (41.25g, y 4.92% (10.40g al primer, segundo y tercer periodo sucesivamente. El descenso de Peso del cuerpo del útero fue de: 76.60% (87.25g, 17.08% (19.45g y 6.32% (7.2g al primer, segundo y tercer periodo sucesivamente. El Peso de reducción gradual de la cervix fue de: 68% (47,15g, 26,10% (18,00g y 5,51% (3,8g al primer, segundo y tercer periodo respectivamente. This study was carried out in the Histology and Embriology laboratory at the Medicine Veterinary and Zootecny of the Altiplano National University, with alpacas coming from the EPS Rural Alliance (4140 m.a.s.l., Quimsachata Research Center (4050 m.a.s.l. and the Raya Research Center (4130 m.a.s.l. whose objective was the study the process of the uterine involution after parturition from day 0 to 30. For such purpose 34 multiparous alpacas from 4 to 6 year old were selectec. The results were: Macroscopically the uterusweight lost 71,07% (385,25g, 25,54% (138,55g and 3,42% (18,6g at first

  6. Water requirements for wheat and maize under climate change in North Nile Delta

    Directory of Open Access Journals (Sweden)

    Samiha Ouda

    2015-03-01

    -M (Penman Monteith; RMSE (root mean square error; WHC (water holding capacity. Citation: Ouda, S.; Noreldin, T.; Abd El-Latif, K. (2015. Water requirements for wheat and maize under climate change in North Nile Delta. Spanish Journal of Agricultural Research, Volume 13, Issue 1, e03-001, 10 pages. http://dx.doi.org/10.5424/sjar/2015131-6412. Received: 15 Jun 2014. Accepted: 02 Dec 2014. http://dx.doi.org/10.5424/sjar/2015131-6412 Copyright © 2015 INIA. This is an open access article distributed under the Creative Commons Attribution License (CC by 3.0, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Funding: The author(s received no specific funding for this work. Competing interests: The authors have declared that no competing interests exist. Correspondence should be addressed to Samiha Ouda: samihaouda@yahoo.com

  7. Analysis of the degradation of biodegradable mulches in a pepper crop under organic management

    Science.gov (United States)

    Moreno, Carmen; González, Sara; Villena, Jaime; Meco, Ramón; María Moreno, Marta

    2016-04-01

    Mimgreen® paper remained practically intact or with little damaged until the end of the crop season, while biopolymers suffered further deterioration, especially Bioflex®. ii) The buried part of the Mimgreen® paper underwent a rapid and complete degradation, while biopolymers presented a variable behavior, from practically intact, similar to polyethylene (Ecovio®), to an important decrease of the soil covered (Mater-Bi® and Sphere 4®). iii) A visual understatement of the functionality of the material (subjective scales) was observed when compared with the numerical value of the percentage of the soil covered by the mulch. Acknowledgements: the research was funded by Project RTA2011-00104-C04-03 from the INIA (Spanish Ministry of Economy and Competitiveness).

  8. Transcriptional regulation of multi-drug tolerance and antibiotic-induced responses by the histone-like protein Lsr2 in M. tuberculosis.

    Directory of Open Access Journals (Sweden)

    Roberto Colangeli

    2007-06-01

    Full Text Available Multi-drug tolerance is a key phenotypic property that complicates the sterilization of mammals infected with Mycobacterium tuberculosis. Previous studies have established that iniBAC, an operon that confers multi-drug tolerance to M. bovis BCG through an associated pump-like activity, is induced by the antibiotics isoniazid (INH and ethambutol (EMB. An improved understanding of the functional role of antibiotic-induced genes and the regulation of drug tolerance may be gained by studying the factors that regulate antibiotic-mediated gene expression. An M. smegmatis strain containing a lacZ gene fused to the promoter of M. tuberculosis iniBAC (PiniBAC was subjected to transposon mutagenesis. Mutants with constitutive expression and increased EMB-mediated induction of PiniBAC::lacZ mapped to the lsr2 gene (MSMEG6065, a small basic protein of unknown function that is highly conserved among mycobacteria. These mutants had a marked change in colony morphology and generated a new polar lipid. Complementation with multi-copy M. tuberculosis lsr2 (Rv3597c returned PiniBAC expression to baseline, reversed the observed morphological and lipid changes, and repressed PiniBAC induction by EMB to below that of the control M. smegmatis strain. Microarray analysis of an lsr2 knockout confirmed upregulation of M. smegmatis iniA and demonstrated upregulation of genes involved in cell wall and metabolic functions. Fully 121 of 584 genes induced by EMB treatment in wild-type M. smegmatis were upregulated ("hyperinduced" to even higher levels by EMB in the M. smegmatis lsr2 knockout. The most highly upregulated genes and gene clusters had adenine-thymine (AT-rich 5-prime untranslated regions. In M. tuberculosis, overexpression of lsr2 repressed INH-mediated induction of all three iniBAC genes, as well as another annotated pump, efpA. The low molecular weight and basic properties of Lsr2 (pI 10.69 suggested that it was a histone-like protein, although it did not

  9. VACCINATION OF HYBRID STRIPED BASS: GROWTH, IMMUNE REACTION AND GENE EXPRESSION

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    Mark Westerman

    2012-10-01

    Full Text Available Hybrid striped bass (42.6+4.9 g wet wt; 139.3+6.1 mm length, were randomly stocked into one of 6 tanks (n=6 tank-1 of a custom designed, recirculating life support system (RLSS. Water quality was as follows: DO2 (6.50.6 mg l-1, pH (7.70.5, TAN (0.06-1.31 mg l-1, nitrite (0.06-0.60 mg l-1 and nitrate (2.0-32.1 mg l-1, salinity 5 ppt, temperature 28±1 oC. A 12:12 photophase:scotophase was used, with a 30 min. dusk-dawn dimming of lights. Fish were fed at 4% body wt d-1 as two separate feedings (08.00 and 16.00 h. Dietary crude protein and lipid levels were 40% and 10% respectively. Tanks were randomly paired and fish either left untreated, vaccinated, or sham injected. The vaccine employed was an experimental formalin killed Streptococcus iniae oil-in-water adjuvanted bacterin. Fish were weighed and measured bimonthly for 8 wk with group weights being employed to adjust feeding rates. At trial termination, all animals were weighed and measured, and their condition factor (CF and feed conversion ratios (FCR calculated. Visceral somatic (VSI and hepatosomatic (HSI indices and intraperitoneal fat (IPF and muscle ratios (MR were also assessed. Blood was taken from anaesthetized fish and hematocrit recorded. Blood was allowed to clot overnight at 5 oC after which serum protein levels were recorded and lysozyme activity measured. Livers were prepared for microarray evaluation using standard techniques using a Danio rerio genechip to assess global changes in gene expression. No differences were observed with respect to final weights, lengths, CF, FCR, or HSI although differences (P < 0.001 were determined for VSI, which was higher in control animals. Packed cell volume and serum protein levels were similar across groups (P > 0.05. Time-course of changes in serum lysozyme activity described an initial reduction in lysozyme activity followed by a rebounding in activity which peaked 25 days post-treatments. Evaluation of lysozyme activity among time

  10. Construction and Identification of Full-length cDNA Library of the Peripheral Blood Leucocytes in Oreochromis niloticus after Vaccination%尼罗罗非鱼免疫后外周血白细胞全长cDNA文库的构建及鉴定

    Institute of Scientific and Technical Information of China (English)

    陈明; 梁万文; 李超; 王瑞; 李莉萍; 甘西; 余晓丽; 雷爱莹; 黄婷; 陈福艳

    2011-01-01

    为大规模快速克隆罗非鱼细胞免疫功能基因,采用SMART技术,构建了尼罗罗非鱼链球菌疫苗免疫后外周血白细胞全长cDNA文库.提取免疫后第3、5和7天外周血白细胞总RNA,用Powerscrip TM反转录酶逆转录合成第一链cDNA,LD-PCR扩增获得双链cDNA,经sfiⅠ酶切和CHROMA SPIN-400TM柱分级分离,收集500 bp以上的片段重组于改造的pBluescriptⅡ SK载体并转化大肠杆菌DH5α,测定文库滴度、重组率及库容量.结果表明,构建的cDNA文库原始库容量1.021×106克隆,文库滴度1.078×106pfu/mL,重组率94.71%,插入片段大小0.75-3.0 kb,平均长度约为1.5 kb.随机对24个克隆测序所获得的18条Contigs进行BLASTx分析,发现有15条Contigs有相关同源信忠,其中10条为全长cDNA,全长串为66.7%.说明所构建文库的各项指标均达到要求,可为筛选罗非鱼免疫功能相关基因和进一步研究基因的结构和功能奠定基础.%In order to rapidly clone the cellular immune function genes of the Tiplia on a large scale, a full-length cDNA library of the peripheral blood leucocytes from the Oreochromis niloticus thud vaccinated of S. iniae vaccine by i. p was constructed by using SMART ( switching mechanism at 5′ end of RNA transcript) techniques. The total RNA was extracted from peripheral blood teucocytes after vaccinated 3 , 5 , and 7 days. The PowerscriptTM reverse transcriptase was used to synthesize and anchor the first-strand cDNA, and the long distance PCR ( LD-PCR) method was used to amplify double-strand cDNA based on the SMART techniquea for construction of a full-length cDNA library. The PCR products were digested by proteinase K. After digestion with Sfi Ⅰ and size fractionation using CHROMA SPIN-400TM columns, cDNAa ( > 500 bp ) were ligated to the Sfi Ⅰ digested , dephosphorylated pBluescript Ⅱ SK vector. The ligation mixture was transformed into E. coil DH5a. The recmnbinant vectors were titered and the recombinant rate

  11. 应用牙科分光光度比色仪对钴铬合金基底表面4种全瓷冠色度学特征的研究%The study of the colorimetric characteristics of the cobalt-chrome alloys abutments covered by four different all-ceramic crowns by using dental spectrophotometer

    Institute of Scientific and Technical Information of China (English)

    陈奕帆; 刘红春; 孟玉坤; 巢永烈; 刘长虹

    2015-01-01

    ObjectiveThis study aims to evaluate the optical data of the different sites of the cobalt-chrome (Co-Cr) alloy abutments covered by four different all-ceramic crowns and the color difference between the crowns and target tab using a digital dental spectrophotometer. Methods   Ten Co-Cr alloy abutments were made and tried in four different groups of all-ceramic crowns, namely, Procera aluminia, Procera zirconia, Lava zirconia (Lava-Zir), and IPS E.max glass-ceramic lithium disilicate-reinforced monolithic. The color data of the cervical, body, and incisal sites of the samples were recorded and analyzed by dental spectrophotometer. The CIE L*, a*, b* values were again measured after veneering. The color difference between the abutments covered by all-ceramic crowns and A2 dentine shade tab was evaluated. Results   The L* and b* values of the abut-ments can be increased by all of the four groups of all-ceramic copings, but a* values were decreased in most groups. A statistical difference was observed among four groups. After being veneered, the L* values of all the copings declined slightly, and the values of a*, b* increased significantly. When compared with A2 dentine shade tab, the ΔΕ of the crowns was below 4. Conclusion   Four ceramic copings were demonstrated to promote the lightness and hue of the alloy abutments effec-tively. Though the colorimetric baseline of these copings was uneven, veneer porcelain can efficiently decrease the color difference between the samples and thee target.%目的:采用牙科分光光度比色仪

  12. Oviposition Preferences of Major Lepidoptera Pest of Pinus kesiya var.langbianensis%思茅松主要鳞翅目害虫的产卵选择性

    Institute of Scientific and Technical Information of China (English)

    马惠芬; 刘凌; 闫争亮; 刘云彩

    2013-01-01

    运用同时蒸馏萃取( SDE)和气相色谱-质谱联用技术( GC-MS)比较了思茅松( Pni usk esiyavar 。 langbianensis)、阿丁枫( Altingia chinensis)和西南桦( Bteu la alnoides)的树叶挥发性物质的组成和含量,并对松实小卷蛾( Rte inia crstata)、微红梢斑螟( Dioryctria rubella)、思茅松毛虫( Dendrolimus kikuchii)在3种树上的产卵选择性进行了试验。 GC-MS分析结果表明:思茅松针叶的挥发性物质以α-蒎烯为主,也含有较多的β-蒎烯、莰烯、石竹烯、月桂烯和β-水芹烯等萜烯类化合物;阿丁枫树叶的挥发物以( E)-石竹烯、异石竹烯、α-依兰油烯、愈创烷-1(5),11-二烯及双环大根香叶烯为主;而西南桦树叶挥发物中,除含有α-蒎烯等萜烯类化合物外,主要以2-己烯醛、2-己烯-1-醇、( Z)-3-己烯-1-醇、( Z)-2-己烯-1-醇等绿叶气味物质为主。产卵选择性试验表明:松实小卷蛾、微红梢斑螟、思茅松毛虫偏好选择思茅松针叶为其产卵场所,而将思茅松喷施阿丁枫和西南桦树叶提取物后,针叶上的产卵量大大减少。说明植物挥发物在松实小卷蛾、微红梢斑螟、思茅松毛虫等鳞翅目松树害虫的产卵寄主选择中起关键作用,松树挥发物提供了寄主对雌虫产卵适宜性的化学信息,而阔叶树挥发物则对其产卵具有驱避作用。%By simultaneous distillation extraction (SDE) and gas chromatography-mass spectrometry (GC-MS), we compared the leaf volatile compositions of Pinus kesiya var.l angbianensis, Altingia chinensis, Betula alnoides and we tested oviposi-tion preferences of Re tiin a csr tata, Dioryctria rubella and Dendrolimus kikuchi .In volatile of Pinus kesiya var.langbianen-sis,α-pinene is the dominant component.β-pinene, camphene,caryophyllene , myrcene and βp-hellandrene are also the maincomponents .In volatile ofA ltingi a chinensis, (E

  13. Estudio macro-microscópico de la involución uterina postparto en Alpacas (Macro and microscopic of uterine involution postpartum in alpacas summary

    Directory of Open Access Journals (Sweden)

    MVZ. Augusto Excelmes Loza.

    2006-04-01

    Full Text Available El presente estudio se llevo a cabo en el Laboratorio de Histología y Embriología de la Facultad de Medicina Veterinaria y Zootecnia de la Universidad Nacional del Altiplano; con alpacas provenientes de la Rural Alianza E.P.S. (4,140m.s.n.m, Centro Experimental Quinsachata-INIA (4,050 m.s.n.m y el Centro de Investigación y Producción La Raya (4,130 m.s.n.m, cuyo objetivo fue el estudio del proceso de la involución uterina post parto en la alpaca, desde el parto hasta los 30dpp. Para tal propósito se estudiaron 34 alpacas multíparas de 4 a 6 años de edad. Los resultados fueron: Macroscópicamente: la perdida de peso del útero fue de: 71.07% (385,25g, 25.54% (138.55g y 3.42% (18.6g al primer, segundo y tercer periodo de la involución uterina. La reducción de Peso del cuerno uterino derecho fue de: 55.26% (37.55g, 38.63% (26.25g y 6.11% (4.15g al primer segundo y tercer periodo respectivamente. La secuencia de la perdida de peso del cuerno uterino izquierdo fue de: 75.50% (159.5g, 19.58% (41.25g, y 4.92% (10.40g al primer, segundo y tercer periodo sucesivamente. El descenso de Peso del cuerpo del útero fue de: 76.60% (87.25g, 17.08% (19.45g y 6.32% (7.2g al primer, segundo y tercer periodo sucesivamente. El Peso de reducción gradual de la cervix fue de: 68% (47,15g, 26,10% 18,00g y 5,51% (3,8g al primer, segundo y tercer periodo respectivamente. Histológicamente: durante los primeros días la superficie uterina es irregular y presenta proyecciones endometriales de aspecto fungiforme y esta cubierto por células pavimentosas, estroma con la presencia de linfocitos, macrófagos y fibroblastos, glándulas reducidas en su diámetro. A partir del sexto día, muestra cambio en el regenerativo, el epitelio de hace predominantemente cúbico, Del sexto al doceavo día el epitelio continua irregular con pequeñas áreas con células epiteliales pavimentosas y cúbicas, lo cual coincide con el cambio en el peso del útero en forma significativa

  14. Distribution and Antimicrobial Susceptibility Analysis of Common Pathogenic Bacteria Isolated from Cold-water Fish in Three North Areas of China%三北地区冷水鱼常见病原菌的分布及药敏试验

    Institute of Scientific and Technical Information of China (English)

    连浩淼; 李绍戊; 张辉; 卢彤岩

    2015-01-01

    because of high density,bad water quality,poor quality of feed and so on,which could also indirectly affect people’ s health. At present,control of bacterial diseases still depends on antibiotics in China,but the abuse of antibiotics will cause drug residues and multi⁃drug resistance of bacteria,so it is necessary for us to study the characteristics of pathogenic bacteria existing in cold⁃water fish aquaculture. In this study,common pathogenic bacteria were isolated and identified from farm⁃raised rainbow trout,Atlantic salmon,silver salmon and Siberia sturgeon in the three north areas of China during the period from May to August in 2013.Based on the epidemiology investigation,different kinds of diseases occurred in fish with different clinical signs:gill⁃rot, haemorrhage in diseased rainbow trout from Qinghai Province;exophthalmosis,darkening in color,saprolegnia⁃sis and bleeding spots in internal organs in diseased silver salmon from Liaoning Province;enteritis,gill⁃rot in Atlantic salmon from Heilongjiang Province and haemorrhage on body surface,mouth irritation,etc. in Siberia sturgeon from Beijing City.Samples were collected and streaked on the plate,and isolated bacteria were inocu⁃lated under aseptic conditions. The results indicated that 12 strains of Aeromonas salmonicida and 1 strain of Acinetobacter were isolated from diseased silver salmon cultured in Liaoning;14 strains of Aeromonas salmonici⁃da,6 strains of Aeromonas sobria,2 strains of Aeromonas hydrophila,6 strains of Acinetobacter,2 strains of Fla⁃vobacterium were isolated from diseased Atlantic salmon cultured in Mudanjiang;9 strains of Aeromonas sobria, 6 strains of Aeromonas veronii,6 strains of Acinetobacter,2 strains of Flavobacterium,2 strains of Yersinia ruckeri were isolated from diseased rainbow trout cultured in Qinghai;3 strains of Streptococcus iniae,3 strains of Acine⁃tobacter 10 strains of Streptococcus dysgalactiae,2 strains of Aeromonas veronii,2 strains of Aeromonas