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Sample records for boto inia geoffrensis

  1. Presumed normal ultrasonographic findings of the testis and epididymis of botos (Inia geoffrensis).

    Science.gov (United States)

    Alves, Flávio Ribeiro; da Silva, Vera Maria Ferreira; Martin, Anthony Richard; Ambrósio, Carlos Eduardo; Giglio, Robson Fortes; Miglino, Maria Angélica

    2012-12-01

    Fifteen live adult male botos, or Amazon river dolphins (Inia geoffrensis), were examined using ultrasonography during the yearly capture expedition, between October and November 2005, at the Mamirauá Sustainable Development Reserve, within the Brazilian Amazon (3 degrees S, 65 degrees W). All examinations were performed with a Sonosite 180 plus ultrasound unit in conjunction with a 2- to 5-MHz multifrequency transducer convex array 180 Plus/Elite-C60. Age and maturity estimates were determined considering the body length, weight, and external characteristics. In all examinations, the testes were discerned by the presence of a hyperechoic central line, called the mediastinum testis, a landmark for their identification during ultrasonography. No significant differences in echogenicity were detected on the ultrasonographic appearance of the testes among the studied animals. On adult male botos, apparent parenchymal nodulation of the testis was observed on scanning in most of the animals and probably constituted evidence of reproductive maturity. Using the color Doppler technique, blood flow was detected along the mediastinum testis that progressively decreased toward the periphery of this organ. Little blood flow could be identified by color Doppler. Power Doppler allowed better accuracy to identify testicular vessels, their topography, and their differentiation from adjacent structures. Ultrasonographic examination provides useful data for morphologic characterization of the boto's testes. Examination using Doppler techniques was considered a valuable tool to evidence blood flow through the testicular parenchyma. PMID:23272345

  2. As atividades turísticas baseadas na alimentação artificial de botos-da-Amazônia (Inia geoffrensis) e a legislação ambiental brasileira

    OpenAIRE

    Luiz Cláudio Pinto de Sá Alves; Carlos José Saldanha Machado; Rodrigo Machado Vilani; Marcelo Derzi Vidal; Artur Andriolo; Alexandre de Freitas Azevedo

    2013-01-01

    Here we evaluate the touristic activity involving the interaction between botos (Inia geoffrensis) and humans based on the conditioning through artificial feeding in the light of the environmental legislation, using the Anavilhanas National Park (ANP) as case study. The Park is located in the Brazilian Amazon, in Novo Airão city, Amazonas State. This activity has significantly increased over the last years, as well as the literature describing its negative effects. By consulting the specializ...

  3. As atividades turísticas baseadas na alimentação artificial de botos-da-Amazônia (Inia geoffrensis e a legislação ambiental brasileira

    Directory of Open Access Journals (Sweden)

    Luiz Cláudio Pinto de Sá Alves

    2013-12-01

    Full Text Available Here we evaluate the touristic activity involving the interaction between botos (Inia geoffrensis and humans based on the conditioning through artificial feeding in the light of the environmental legislation, using the Anavilhanas National Park (ANP as case study. The Park is located in the Brazilian Amazon, in Novo Airão city, Amazonas State. This activity has significantly increased over the last years, as well as the literature describing its negative effects. By consulting the specialized literature, analyzing the legal framework and empirical material, it was verified that i legally speaking this practice presents conflicts to some aspects of the Brazilian environmental legislation, ecotourism concepts and functions of the Conservation Units and, ii administratively speaking, the environmental agencies are now taking appropriate steps to mitigate the negative impacts and gradual actions in order to alter the touristic activities to comply with the current environmental legislation. We conclude that there is still a lot to be done for the botos tourism model that occurs in the Brazilian Amazon to be conducted in a sustainable manner, suggesting that the group of heterogeneous actions proposed here be implemented to achieve that.

  4. Molecular cytogenetic characterization of the Amazon River dolphin Inia geoffrensis.

    Science.gov (United States)

    Bonifácio, Heidi L; da Silva, Vera M F; Martin, Anthony R; Feldberg, Eliana

    2012-09-01

    Classical and molecular cytogenetic (18S rDNA, telomeric sequence, and LINE-1 retrotransposon probes) studies were carried out to contribute to an understanding of the organization of repeated DNA elements in the Amazon River dolphin (boto, Inia geoffrensis). Twenty-seven specimens were examined, each presenting 2n = 44 chromosomes, the karyotype formula 12m + 14sm + 6st + 10t + XX/XY, and fundamental number (FN) = 74. C-positive heterochromatin was observed in terminal and interstitial positions, with the occurrence of polymorphism. Interstitial telomeric sequences were not observed. The nucleolar organizer region (NOR) was located at a single site on a smallest autosomal pair. LINE-1 was preferentially distributed in the euchromatin regions, with the greatest accumulation on the X chromosome. Although the karyotype structure in cetaceans is considered to be conserved, the boto karyotype demonstrated significant variations in its formula, heterochromatin distribution, and the location of the NOR compared to other cetacean species. These results contribute to knowledge of the chromosome organization in boto and to a better understanding of karyoevolution in cetaceans.

  5. Stomach Content of a Juvenile Bolivian River Dolphin (Inia geoffrensis boliviensis) from the Upper Madeira Basin, Bolivia

    NARCIS (Netherlands)

    Aliaga-Rossel, E.; Beerman, A.S.; Sarmiento, J.

    2010-01-01

    The article presents a study about the stomach content of a juvenile Bolivian river dolphin (Inia geoffrensis boliviensis), an endemic subspecies of the Amazon River dolphin, found in the upper Madeira River basin in Bolivia. The study finds that the stomach of Bolivian river dolphin contained a mix

  6. Seroprevalence of Toxoplasma gondii in free-living Amazon river dolphins (Inia geoffrensis) from central Amazon, Brazil

    Science.gov (United States)

    Toxoplasma gondii is an important pathogen in aquatic mammals and its presence in these animals may indicate water contamination of aquatic environment by oocysts. Serum samples from 95 dolphins from free-living Amazon River dolphins (Inia geoffrensis) from Sustainable Development Reserve Mamirauá (...

  7. Seroprevalence of Toxoplasma gondii in free-living amazon river dolphins (Inia geoffrensis) from central Amazon, Brazil

    Science.gov (United States)

    Toxoplasma gondii is an important pathogen in aquatic mammals and its presence in these animals may indicate water contamination of aquatic environment by oocysts. Serum samples from 95 dolphins from free-living Amazon River dolphins (Inia geoffrensis) from Sustainable Development Reserve Mamirauá (...

  8. Seroprevalence of Toxoplasma gondii in free-living Amazon River dolphins (Inia geoffrensis) from central Amazon, Brazil.

    Science.gov (United States)

    Santos, P S; Albuquerque, G R; da Silva, V M F; Martin, A R; Marvulo, M F V; Souza, S L P; Ragozo, A M A; Nascimento, C C; Gennari, S M; Dubey, J P; Silva, J C R

    2011-12-29

    Toxoplasma gondii is an important pathogen in aquatic mammals and its presence in these animals may indicate the water contamination of aquatic environment by oocysts. Serum samples from 95 free-living Amazon River dolphins (Inia geoffrensis) from the Mamirauá Sustainable Development Reserve (RDSM), Tefé, Amazonas, Central Amazon, Brazil were tested for T. gondii antibodies using the modified agglutination test (MAT). Antibodies (MAT ≥ 25) to T. gondii were found in 82 (86.3%) dolphins with titers of 1:25 in 24, 1:50 in 56, and 1:500 in 2. Results suggest a high level contamination of the aquatic environment of the home range of these animals. PMID:21764516

  9. Placentation in dolphins from the Amazon River Basin: the Boto, Inia geoffrensis, and the Tucuxi, Sotalia fluviatilis

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    Bonatelli Marina

    2007-06-01

    Full Text Available Abstract A recent reassessment of the phylogenetic affinities of cetaceans makes it timely to compare their placentation with that of the artiodactyls. We studied the placentae of two sympatric species of dolphin from the Amazon River Basin, representing two distinct families. The umbilical cord branched to supply a bilobed allantoic sac. Small blood vessels and smooth muscle bundles were found within the stroma of the cord. Foci of squamous metaplasia occurred in the allanto-amnion and allantochorion. The interhemal membrane of the placenta was of the epitheliochorial type. Two different types of trophoblastic epithelium were seen. Most was of the simple columnar type and indented by fetal capillaries. However, there were also areolar regions with tall columnar trophoblast and these were more sparsely supplied with capillaries. The endometrium was well vascularised and richly supplied with actively secreting glands. These findings are consistent with the current view that Cetacea are nested within Artiodactyla as sister group to the hippopotamids.

  10. Amazon river dolphins (Inia geoffrensis) use a high-frequency short-range biosonar

    DEFF Research Database (Denmark)

    Ladegaard, Michael; Jensen, Frants Havmand; de Freitas, Mafalda;

    2015-01-01

    to interpret in acoustically complex habitats, echolocation clicks of wild Amazon river dolphins were recorded using a vertical seven-hydrophone array. We identified 404 on-axis biosonar clicks having a mean SLpp of 190.3±6.1 dB re. 1 μPa, mean SLEFD of 132.1±6.0 dB re. 1 μPa2s, mean Fc of 101.2±10.5 kHz, mean...

  11. Vocalizations of Amazon river dolphins (Inia geoffrensis) : Characterization, effect of physical environment and differences between populations

    OpenAIRE

    Amorim, Thiago Orion Simões; Andriolo, Artur; Reis, Sarah S.; Santos, Manuel Eduardo dos

    2016-01-01

    The vocal repertoire of the Amazon river dolphin and its geographic variations are still poorly known, especially in relation to ecological variables. Here the acoustic characteristics of low frequency pulsed vocalizations, with single or multiple pulses, recorded in two protected areas of the Amazon were described and differences in acoustic emissions related to water properties were analyzed. Both frequency and time parameters differ relative to abiotic condition of water turbid...

  12. Notes on the nasal tract complex of the Boutu, Inia geoffrensis (De Blainville, 1817) (Cetacea, Platanistidae)

    NARCIS (Netherlands)

    Schenkkan, E.J.

    1977-01-01

    The anatomy of the nasal passage of three specimens of the Boutu has been studied. The various diverticula appear to be aberrant from those described for other Platanistidae. The vestibular airsac system has developed into one enormous diverticulum. Except for a narrow region caudal to the blowhole,

  13. Streptococcus iniae and Streptococcus agalactiae

    Science.gov (United States)

    Streptococcus iniae and S. agalactiae are economically important Gram positive bacterial pathogens of cultured and wild fish with a worldwide distribution. Both bacteria are potential zoonotic pathogens and have been associated most often with infections in immunocompromised people. Streptococcus in...

  14. DOLLINCO-INIA: TRIGO ALTERNATIVO PARA EL SUR DE CHILE Dollinco-INIA: an alternative wheat for Southern Chile

    OpenAIRE

    Cristian Hewstone M.; Claudio Jobet F.

    2001-01-01

    Dollinco-INIA es un trigo de pan (Triticum aestivum L), semienano, de hábito de desarrollo alternativo, obtenido por el Proyecto de Fitomejoramiento del Instituto de Investigaciones Agropecuarias (INIA), Centro Regional de Investigación Carillanca, Temuco. Es moderadamente susceptible a polvillo estriado, resistente a polvillo de la hoja y moderadamente resistente a septoriosis y oidio. Su rendimiento promedio en ensayos en cuatro temporadas ha fluctuado entre 7,84 y 14,46 t ha-1 en tres loca...

  15. Perception of local inhabitants regarding the socioeconomic impact of tourism focused on provisioning wild dolphins in Novo Airao, Central Amazon, Brazil

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    LUIZ C.P.S. ALVES

    2013-11-01

    Full Text Available Botos (Inia geoffrensis are currently provisioned for use in tourist attractions in five sites in the Brazilian Amazon. Despite the known negative effects associated with human-wild dolphin interactions, this activity has been regulated and licensed in the Anavilhanas National Park in Novo Airão, Amazonas State, Brazil. We present an updated evaluation of the perception of the local community concerning the possible socioeconomic impacts of this tourism in Novo Airão. In April 2011, 45 interviews were conducted with inhabitants. A small segment of Novo Airão perceives currently itself as being economically dependent on the botos feeding tourism. Despite that, the economic benefits of this controversial activity apparently are not shared among most inhabitants, and botos feeding tourism is perceived as generating diverse negative effects. We conclude that if the activity was banned or modified into a less impacting tourist activity, this action would probably not majorly affect the lives of the general population.

  16. Conflicts between river dolphins (Cetacea: Odontoceti and fisheries in the Central Amazon: a path toward tragedy?

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    Luiz Cláudio Pinto de Sá Alves

    2012-10-01

    Full Text Available Dolphin interactions with fishermen have increased significantly and pose potential risks to the boto, Inia geoffrensis (Blainville, 1817, and the tucuxi, Sotalia fluviatilis (Gervais & Deville, 1853. The main objective of the present paper was to describe the existing conflicts between river dolphins and fishermen in the municipality of Manacapuru region. Sixteen fishermen were interviewed in Manacapuru, state of Amazonas, Brazil who described a situation of ongoing conflict that may be unsustainable. Two merchants from Manacapuru made unconfirmed reports on a boto carcass trade. Data collection for this study occurred between April 20th and April 25th, 2009, but the first author had been conducting research on river dolphins and fisheries in Manacapuru and nearby cities since the beginning of 2008, in order to gain the trust of the fishermen interviewed. The hunting and deliberate killing of the species is probably more threatening to botos than their incidental capture in fishing gears in the Manacapuru region. This practice may result from the fact that dolphins are prone to damaging fishing equipment, and stealing (and possibly damaging fish from the nets. They are portrayed negatively in numerous myths and superstitions of traditional Amazonian folklore, making them extremely undesired or even hated, seen as pests, and used in the piracatinga, Calophysus macropterus (Lichtenstein, 1819 fishery as bait. For tucuxis, incidental capture still represents the major threat to their conservation in the region evaluated here.

  17. Sil: a Streptococcus iniae bacteriocin with dual role as an antimicrobial and an immunomodulator that inhibits innate immune response and promotes S. iniae infection.

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    Mo-fei Li

    Full Text Available Streptococcus iniae is a Gram-positive bacterium and a severe pathogen to a wide range of economically important fish species. In addition, S. iniae is also a zoonotic pathogen and can cause serious infections in humans. In this study, we identified from a pathogenic S. iniae strain a putative bacteriocin, Sil, and examined its biological activity. Sil is composed of 101 amino acid residues and shares 35.6% overall sequence identity with the lactococcin 972 of Lactococcus lactis. Immunoblot analysis showed that Sil was secreted by S. iniae into the extracellular milieu. Purified recombinant Sil (rSil exhibited a dose-dependent inhibitory effect on the growth of Bacillus subtilis but had no impact on the growths of other 16 Gram-positive bacteria and 10 Gram-negative bacteria representing 23 different bacterial species. Treatment of rSil by heating at 50°C abolished the activity of rSil. rSil bound to the surface of B. subtilis but induced no killing of the target cells. Cellular study revealed that rSil interacted with turbot (Scophthalmus maximus head kidney monocytes and inhibited the innate immune response of the cells, which led to enhanced cellular infection of S. iniae. Antibody blocking of the extracellular Sil produced by S. iniae significantly attenuated the infectivity of S. iniae. Consistent with these in vitro observations, in vivo study showed that administration of turbot with rSil prior to S. iniae infection significantly increased bacterial dissemination and colonization in fish tissues. Taken together, these results indicate that Sil is a novel virulence-associated bacteriostatic and an immunoregulator that promotes S. iniae infection by impairing the immune defense of host fish.

  18. Controlled laboratory challenge demonstrates substantial additive genetic variation in resistance to Streptococcus iniae in Nile tilapia

    Science.gov (United States)

    Streptococcus iniae is an etiologic agent of streptococcal disease in tilapia and is one of several Streptococcus spp. that negatively impact worldwide tilapia production. Methods for the prevention and control of S. iniae include vaccines, management strategies, and antibiotics. An alternative and ...

  19. Immunoproteomic analysis of the antibody response obtained in tilapia following immunization with a Streptococcus iniae vaccine

    Science.gov (United States)

    Streptococcus iniae is one of the most economically important Gram-positive pathogens in cultured fish species worldwide. Research has shown that vaccination is a tool that can be used in the prevention of streptococcal disease. The USDA-ARS patented S. iniae vaccine has been demonstrated to be ef...

  20. Protective efficiency of an inactivated vaccine against Streptococcus iniae in olive flounder, Paralichthys olivaceus

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    Jeong Yong-Uk

    2016-03-01

    Full Text Available Streptococcus iniae is a causative agent of hemorrhagic septicemia in olive flounder, Paralichthys olivaceus, in Korea, resulting in serious economic losses. As a preventive measure, M VAC INIAE (Mastuken, Japan was prepared from the S. iniae F2K strain and tested against the SI-36 strain prevalent on flounder fish farms on Jeju Island, Korea. F2K had a serotype of 38 (− and SI-36 38 (+. The vaccine recognized both serotypes. It showed a very high effective immune response against S. iniae; the challenge test using the S. iniae SI-36 strain resulted in a relative percent survival (RPS of 85.7-87.0% 2 weeks after vaccination and 71.0-80.0% 6 months after vaccination. Field vaccination and clinical challenge tests were performed at local Jeju aquafarms with S. iniae SI-36. These showed significantly reduced cumulative mortality when compared to the control group with RPS rates that ranged between 71-80%. Hence, the present study suggests that this vaccine showed a significant immune response against S. iniae and could be applied in commercial aquafarms as a therapeutic agent against β-hemolytic streptococcosis in cultured P. olivaceus.

  1. Bath immersion, booster vaccination strategy holds potential for protecting juvenile tilapia against Streptococcus iniae

    Science.gov (United States)

    Streptococcus iniae is a significant bacterial pathogen that causes hemorrhagic septicemia and meningoencephalitis in tilapia, hybrid striped bass, rainbow trout, olive flounder, yellowtail, barramundi and other species of cultured and wild fish worldwide. In tilapia production, vaccination of fry ...

  2. Antibacterial activity of Iranian medicinal plants against Streptococcus iniae isolated from rainbow trout (Oncorhynchus mykiss

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    Pirbalouti Ghasemi Abdollah

    2011-01-01

    Full Text Available Streptococcus iniae is among the major pathogens of a large number of fish species cultured in fresh and marine recirculating and net pen production systems. Ten Iranian medicinal plants were assessed for their antimicrobial activity against Streptococcus iniae isolates obtained from diseased Oncorhynchus mykiss (Salmonidae; Walbaum, 1972 collected from fish farms in Iran. The antibacterial activity of ethanol extracts of Punica granatum, Quercus branti, Glycyrrhiza glabra and essential oils of Heracleum lasiopetalum, Satureja bachtiarica, Thymus daenensis, Myrtus communis, Echinophora platyloba, Kelussia odoratissima and Stachys lavandulifolia against Steptococcus iniae was evaluated by disc diffusion and serial dilution assays. Most of the extracts and essential oils showed a relatively high antibacterial activity against Streptococcus iniae. Of the plants studied, the most active extracts were those obtained from the essential oils of Satureja bachtiarica, Echinophora platyloba, Thymus daenensis and the ethanol extract of Quercus branti. Some of the extracts were active against Streptococcus iniae. Two essential oils showed lower MIC values; Heracleum lasiopetalum (78 μg/ml and Satureja bachtiarica (39 μg/ml. The essential oil of Satureja bachtiarica could be an important source of antibacterial compounds against the Streptococcus iniae isolated from rainbow trout.

  3. Kipa-INIA, new high yield spring bread wheat variety for Chile Kipa-INIA, nueva variedad de trigo harinero de primavera de alto rendimiento para Chile

    OpenAIRE

    Iván Matus; Ricardo Madariaga; Claudio Jobet; Javier Zúñiga; Christian Alfaro

    2011-01-01

    Kipa-INIA is a spring wheat variety (Triticum aestivum L.) originated from a cross carried out in the Wheat Plant Breeding Project of the Instituto de Investigaciones Agropecuarias (INIA), in the Centro Regional de Investigación Quilamapu (36°31’ S; 71°54’ W) in 1993. It has an upright growth habit in the seedling stage. The adult plant is low to medium height and varies between 90 and 95 cm. The spike is white with long awns along its full length. The grain is ovate, white, and vitreous. It ...

  4. Controlled challenge experiment demonstrates substantial additive genetic variation in resistance of Nile tilapia (Oreochromis niloticus) to Streptococcus iniae

    Science.gov (United States)

    Streptococcus iniae is an etiologic agent of streptococcal disease in tilapia and is one of several Streptococcus spp. that negatively impact worldwide tilapia production. Methods for the prevention and control of S. iniae include vaccines, management strategies, and antibiotics. A complimentary pre...

  5. Development and efficacy of a noviobiocin-resistant Streptococcus iniae as a novel vaccine in Nile tilapia (Oreochromis niloticus)

    Science.gov (United States)

    A novel attenuated Streptococcus iniae vaccine was developed from a virulent strain of Streptococcus iniae (ISET0901) through selection for novobiocin resistance (therefore named ISNO). The safety of ISNO was then evaluated in Nile tilapia (Oreochromis niloticus) through intraperitoneal (IP) injecti...

  6. Development and efficacy of a novobiocin-resistant Streptococcus iniae as a novel vaccine in Nile tilapia (Oreochromis niloticus)

    Science.gov (United States)

    A novel attenuated Streptococcus iniae vaccine was developed from a virulent strain of Streptococcus iniae through selection for novobiocin resistance. The safety of the novel vaccine (named ISNO) was then evaluated in Nile tilapia (Oreochromis niloticus) through intraperitoneal (IP) injection. When...

  7. Immunoproteomic analysis of the antibody response obtained in Nile tilapia following vaccination with a Streptococcus iniae vaccine

    Science.gov (United States)

    Streptococcus iniae is one of the most economically important Gram-positive pathogens in cultured fish species worldwide. The USDA-ARS Aquatic Animal Health Research Unit developed a modified (contains concentrated culture supernatant) S. iniae bacterin that has been demonstrated to be efficacious,...

  8. Immunoproteomic analysis of the antibody response obtained in Nile tilapia following vaccination with a Streptococcus iniae vaccine.

    Science.gov (United States)

    LaFrentz, Benjamin R; Shoemaker, Craig A; Klesius, Phillip H

    2011-09-28

    Streptococcus iniae is one of the most economically important Gram-positive pathogens in cultured fish species worldwide. The USDA-ARS Aquatic Animal Health Research Unit developed a modified (contains concentrated culture supernatant) S. iniae bacterin that has been demonstrated to be efficacious, and protection is mediated by specific anti-S. iniae antibodies. Although effective, the specific vaccine components important for efficacy are not known. In the present study, an immunoproteomic approach was utilized to identify whole-cell lysate proteins of S. iniae that stimulated specific antibody production in Nile tilapia (Oreochromis niloticus) following vaccination. Groups of tilapia were vaccinated by intraperitoneal injection with the modified S. iniae bacterin or were mock-vaccinated, and at 30 d post-vaccination sera samples were obtained from individual fish. Vaccination of tilapia with the S. iniae vaccine stimulated significantly elevated specific antibody responses against proteins of the bacterium and passive immunization of tilapia with this serum demonstrated the antibodies were highly protective. Whole-cell lysate proteins of S. iniae were separated by 2D-PAGE and were probed with a pooled serum sample from vaccinated tilapia. A total of eleven unique immunogenic proteins were positively identified by mass spectrometry. Based on research conducted on homologous proteins in other Streptococcus spp., antibodies specific for three of the identified proteins, enolase, glyceraldehyde-3-phosphate dehydrogenase, and fructose-bisphosphate aldolase, are likely involved in protection from streptococcosis caused by S. iniae. PMID:21601381

  9. Rapid Detection and Identification of Streptococcus Iniae Using a Monoclonal Antibody-Based Indirect Fluorescent Antibody Technique

    Science.gov (United States)

    Streptococcus iniae is among the major pathogens of a large number of fish species cultured in fresh and marine recirculating and net pen production systems . The traditional plate culture technique to detect and identify S. iniae is time consuming and may be problematic due to phenotypic variations...

  10. Fast induction of nisin resistance in Streptococcus thermophilus INIA 463 during growth in milk.

    Science.gov (United States)

    Garde, Sonia; Avila, Marta; Medina, Margarita; Nuñez, Manuel

    2004-11-01

    Streptococcus thermophilus INIA 463 became nisin-resistant after exposure in skim milk to subminimal inhibitory concentrations of nisin (1-3 IU/ml) for less than 2 h. Addition of 20 IU/ml caused a 4 log unit decrease in S. thermophilus counts of a culture not exposed previously to nisin, whereas no decrease was observed in the culture exposed to nisin for 2 h. Transfer of immunity genes as responsible for nisin resistance was discarded. The presence of extracellular or intracellular specific nisin-degrading enzymes was not detected in the nisin-resistant variant of S. thermophilus INIA 463. Nisin resistance was caused by the induction of a resistance mechanism. Transmission electron microscopy (TEM) revealed that the nisin-resistant variant of S. thermophilus INIA 463 had a thickened cell wall compared to the wild strain. Resistance to nisin was lost after one transfer (4 h growth) in nisin-free skim milk. PMID:15364470

  11. Genetic Characterization of Streptococcus iniae in Diseased Farmed Rainbow Trout (Onchorhynchus mykiss in Iran

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    A. Erfanmanesh

    2012-01-01

    Full Text Available Genetic characterization of strains of Streptococcus iniae recovered from morbidity and mortality of farmed rainbow trout in different provinces of Iran were studied. The Gram-positive cocci isolates were obtained from the kidney tissues of diseased rainbow trout on blood agar at 25°C for 72 h. The grown bacteria were then characterized using biochemical and molecular works. The identified 26 isolates of S. iniae producing a 513 bp in PCR procedure were then compared using random amplified polymorphic DNA (RAPD analysis using 9 random primers. The phylogenetic tree of the RAPD product using UPMGA software included these strains in one genetic group but into two clusters. The results of this study show that S. iniae strains from the diseased rainbow trout in the north part of Iran are genetically similar to those strains in the south and west parts of the country.

  12. Kipa-INIA, new high yield spring bread wheat variety for Chile Kipa-INIA, nueva variedad de trigo harinero de primavera de alto rendimiento para Chile

    Directory of Open Access Journals (Sweden)

    Iván Matus

    2011-06-01

    Full Text Available Kipa-INIA is a spring wheat variety (Triticum aestivum L. originated from a cross carried out in the Wheat Plant Breeding Project of the Instituto de Investigaciones Agropecuarias (INIA, in the Centro Regional de Investigación Quilamapu (36°31’ S; 71°54’ W in 1993. It has an upright growth habit in the seedling stage. The adult plant is low to medium height and varies between 90 and 95 cm. The spike is white with long awns along its full length. The grain is ovate, white, and vitreous. It was sown in mid-August in the Santa Rosa Experimental Field, Chillán, head emergence occurred 90 to 95 d after sowing, i.e. is 4 to 6 d before ‘Domo-INIA’. On the mean, ‘Kipa-INIA’ sown under irrigation conditions reached a yield of 11.7% higher than the control var. Domo-INIA, and 18.1% higher in dryland soils.Kipa-INIA es un trigo harinero (Triticum aestivum L. de primavera que proviene de un cruzamiento efectuado el año 1993 en el Proyecto de Fitomejoramiento de Trigo del Instituto de Investigaciones Agropecuarias (INIA, en el Centro Regional de Investigación Quilamapu. Es un trigo con hábito de crecimiento erecto al estado de plántula. La altura de la planta adulta se considera mediana, y varía entre 90 y 95 cm. La espiga es de color blanco, de barbas largas presentes en toda su extensión. El grano es de color blanco, aspecto vítreo, y forma ovada. Sembrado a mediados de agosto en el Campo Experimental Santa Rosa (36°31’ S; 71°54’ O, Chillán, la emisión de espigas ocurre 90 a 95 días después de la siembra, entre 4 a 6 días antes que ‘Domo-INIA’. En promedio sembrado bajo condiciones de riego, ‘Kipa-INIA’ alcanzó un rendimiento medio 11,7% mayor que la variedad testigo Domo-INIA y en suelos de secano tuvo un rendimiento superior de un 18,1% respecto de ‘Domo-INIA’.

  13. Laboratory efficacy of florfenicol against Streptococcus iniae infection in sunshine bass

    Science.gov (United States)

    An experimental feeding trial was performed to evaluate the efficacy of florfenicol (FF) in controlling Streptococcus iniae infection in sunshine bass (SB). Doses of FF tested were 0, 5, 10, 15 and 30 mg active ingredient per kilogram of fish body weight (BW) per day. Administration of medicated f...

  14. Laboratory efficacy of oxytetracyline and amoxicillin for the control of Streptococcus iniae infection in tilapia

    Science.gov (United States)

    Streptococcus iniae infection of tilapia is common in different parts of the world including the United States. The infection has caused devastating economic losses and the closure of many tilapia operations. Proper husbandry and health management practices are essential but when a virulent outbre...

  15. Fatal septicemia caused by the zoonotic bacterium Streptococcus iniae during an outbreak in Caribbean reef fish

    Science.gov (United States)

    An outbreak of Streptococcus iniae occurred in the early months of 2008 among wild reef fish in the waters of the Federation of St.Kitts and Nevis, lasting almost 2 months. Moribund and dead fish were collected for gross, histological, bacteriological, and molecular analysis. Necropsy findings inclu...

  16. Effectiveness of early intervention with florfenicol on a Streptococcus iniae infection in blue tilapia

    Science.gov (United States)

    An experimental feeding trial was performed to assess the efficacy of florfenicol (FFC) in controlling Streptococcus iniae infection in blue tilapia, Oreochromis aureus. Doses of FFC tested were 0, 5, 10, 15 and 30 mg active ingredient per kilogram of fish body weight (BW) per day. Administration ...

  17. Effect of parasitism on vaccine efficacy against Streptococcus iniae in Nile tilapia

    Science.gov (United States)

    Limited information is available on vaccine performance in parasitized fish. The objective of this study was to determine if parasitism of fish affected vaccine efficacy. Antibody level, hematology and survival of Nile tilapia vaccinated with a modified S. iniae bacterin were compared among non-para...

  18. Experimental study to evaluate the pathogenicity of Streptococcus iniae in Guppy (Poecilia reticulata

    Directory of Open Access Journals (Sweden)

    Milad Adel

    2015-01-01

    Full Text Available Streptococcus iniae has emerged as an important fish pathogen over the last decade in farmed rainbow trout in Iran. The main objective of this study was to evaluate the pathogenicity of S. iniae in Poecilia reticulata. Atotal of 60 apparently healthy P. reticulata were obtained from ornamental fish pet store and injected intraperitoneally with 1.5×106 cfu of bacteria. For 14 days after challenge, the rate of mortality and clinical signs were recorded. The first clinical signs was observed in challenged fish 48 hrs after injection of S. iniae and first mortality was observed 72 hrs after injection. No significant differences in mortality and clinical signs between both sexes were observed. Streptococcus iniae was collected from internal organs of fishes challenged, and was confirmed using the conventional biochemical tests and PCR. It is concluded that, P. reticulata is susceptible to streptococcosis and can play an important role in transmission of the disease to other ornamental fish species and also cultured fish.

  19. Streptococcus iniae SF1: complete genome sequence, proteomic profile, and immunoprotective antigens.

    Directory of Open Access Journals (Sweden)

    Bao-cun Zhang

    Full Text Available Streptococcus iniae is a Gram-positive bacterium that is reckoned one of the most severe aquaculture pathogens. It has a broad host range among farmed marine and freshwater fish and can also cause zoonotic infection in humans. Here we report for the first time the complete genome sequence as well as the host factor-induced proteomic profile of a pathogenic S. iniae strain, SF1, a serotype I isolate from diseased fish. SF1 possesses a single chromosome of 2,149,844 base pairs, which contains 2,125 predicted protein coding sequences (CDS, 12 rRNA genes, and 45 tRNA genes. Among the protein-encoding CDS are genes involved in resource acquisition and utilization, signal sensing and transduction, carbohydrate metabolism, and defense against host immune response. Potential virulence genes include those encoding adhesins, autolysins, toxins, exoenzymes, and proteases. In addition, two putative prophages and a CRISPR-Cas system were found in the genome, the latter containing a CRISPR locus and four cas genes. Proteomic analysis detected 21 secreted proteins whose expressions were induced by host serum. Five of the serum-responsive proteins were subjected to immunoprotective analysis, which revealed that two of the proteins were highly protective against lethal S. iniae challenge when used as purified recombinant subunit vaccines. Taken together, these results provide an important molecular basis for future study of S. iniae in various aspects, in particular those related to pathogenesis and disease control.

  20. Cloning and Characterization of Surface-Localized α-Enolase of Streptococcus iniae, an Effective Protective Antigen in Mice

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    Jun Wang

    2015-06-01

    Full Text Available Streptococcus iniae is a major fish pathogen that can also cause human bacteremia, cellulitis and meningitis. Screening for and identification of protective antigens plays an important role in developing therapies against S. iniae infections. In this study, we indicated that the α-enolase of S. iniae was not only distributed in the cytoplasm and associated to cell walls, but was also secreted to the bacterial cell surface. The functional identity of the purified recombinant α-enolase protein was verified by its ability to catalyze the conversion of 2-phosphoglycerate (2-PGE to phosphoenolpyruvate (PEP, and both the recombinant and native proteins interacted with human plasminogen. The rabbit anti-rENO serum blockade assay shows that α-enolase participates in S. iniae adhesion to and invasion of BHK-21 cells. In addition, the recombinant α-enolase can confer effective protection against S. iniae infection in mice, which suggests that α-enolase has potential as a vaccine candidate in mammals. We conclude that S. iniae α-enolase is a moonlighting protein that also associates with the bacterial outer surface and functions as a protective antigen in mice.

  1. CAUQUENES-INIA, NUEVO CULTIVAR DE HUALPUTRA CHILENA (Medicago polymorpha) PARA AREAS DE SECANO MEDITERRANEO. Cauquenes-INIA, a new Chilean cultivar of burr medic (Medicago polymorpha) for Mediterranean dryland areas.

    OpenAIRE

    Carlos Ovalle M; Alejandro del Pozo L.; Julia Avendaño R.; Teresa Aravena; M. Elena Díaz

    2001-01-01

    Cauquenes INIA es el primer cultivar de hualputra (Medicago polymorpha L.), liberada por el Centro Experimental Cauquenes del Instituto de Investigaciones Agropecuarias (INIA). Fue seleccionado desde una colección de germoplasma que obtenida en una expedición a través de la zona Mediterránea de Chile, entre La Serena (29º 55’ lat. S) y Temuco (38º 47’ lat. S), en 1988. Es una leguminosa forrajera anual, para zonas de secano mediterráneo subhúmedo y húmedo, especialmente apropiada para sistema...

  2. Lleuque-INIA, New High Yield Spring Durum Wheat Variety for Chile Lleuque-INIA, Nueva Variedad de Trigo Candeal de Primavera de Alto Potencial de Rendimiento para Chile

    Directory of Open Access Journals (Sweden)

    Iván Matus

    2011-06-01

    Full Text Available The Lleuque-INIA spring durum wheat variety (Triticum turgidum var. durum L. originated from a cross carried out by the Instituto de Investigaciones Agropecuarias (INIA, National Wheat Program, in the Centro Regional de Investigación La Platina, Santiago, Chile, in 1993. This variety has an upright growth habit in the seedling stage; the adult plant is of medium height and varies between 80 and 90 cm. The spike is medium-sized, white, and exhibits long whitish awns along its full length. The grain is large-sized, elongated, golden yellow, and vitreous. The variety was sown in mid-August in the Santa Rosa Experimental Station (36°31’ S; 71°54’ W, Chillán, head emergence occurred between 89 and 91 d after sowing, and was 6 to 8 d later than ‘Llareta-INIA’ and ‘Corcolén-INIA’. In La Platina, ‘Lleuque-INIA’ had a mean yield of 16.7 and 20.8% higher than vars. Llareta-INIA and Corcolén-INIA, respectively, whereas the yield was higher in Chillán by 10.3 and 10.1%, respectively. On the other hand, in Yungay located in the Ñuble foothills, ‘Lleuque-INIA’ yield exceeded ‘Llareta-INIA’ by 13.07% and var. Corcolén-INIA by 16.97%. In Humán, var. Lleuque-INIA yield exceeded the control vars. Llareta-INIA and Corcolén-INIA by 15.23 and 24.03%, respectively. Lleuque-INIA variety is a type of wheat with good hectoliter weight, good wet gluten content (%, and a protein value mean fluctuating between 10.47 and 12.3%.La variedad de trigo candeal (Triticum turgidum var. durum L. de primavera Lleuque-INIA proviene de un cruzamiento efectuado el año 1993 por el Programa Nacional de Trigo del Instituto de Investigaciones Agropecuarias INIA en el Centro Regional de Investigación La Platina, Santiago, Chile. Es un trigo con hábito de crecimiento erecto al estado de plántula, la planta adulta es de altura mediana y varía entre 80 y 90 cm. La espiga es de tamaño medio, compacta, de color blanco, presenta barbas largas de color

  3. Streptococcus iniae M-like protein contributes to virulence in fish and is a target for live attenuated vaccine development.

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    Jeffrey B Locke

    Full Text Available BACKGROUND: Streptococcus iniae is a significant pathogen in finfish aquaculture, though knowledge of virulence determinants is lacking. Through pyrosequencing of the S. iniae genome we have identified two gene homologues to classical surface-anchored streptococcal virulence factors: M-like protein (simA and C5a peptidase (scpI. METHODOLOGY/PRINCIPAL FINDINGS: S. iniae possesses a Mga-like locus containing simA and a divergently transcribed putative mga-like regulatory gene, mgx. In contrast to the Mga locus of group A Streptococcus (GAS, S. pyogenes, scpI is located distally in the chromosome. Comparative sequence analysis of the Mgx locus revealed only one significant variant, a strain with an insertion frameshift mutation in simA and a deletion mutation in a region downstream of mgx, generating an ORF which may encode a second putative mga-like gene, mgx2. Allelic exchange mutagenesis of simA and scpI was employed to investigate the potential role of these genes in S. iniae virulence. Our hybrid striped bass (HSB and zebrafish models of infection revealed that M-like protein contributes significantly to S. iniae pathogenesis whereas C5a peptidase-like protein does not. Further, in vitro cell-based analyses indicate that SiMA, like other M family proteins, contributes to cellular adherence and invasion and provides resistance to phagocytic killing. Attenuation in our virulence models was also observed in the S. iniae isolate possessing a natural simA mutation. Vaccination of HSB with the Delta simA mutant provided 100% protection against subsequent challenge with a lethal dose of wild-type (WT S. iniae after 1,400 degree days, and shows promise as a target for live attenuated vaccine development. CONCLUSIONS/SIGNIFICANCE: Analysis of M-like protein and C5a peptidase through allelic replacement revealed that M-like protein plays a significant role in S. iniae virulence, and the Mga-like locus, which may regulate expression of this gene, has an

  4. Protection induced by a CpG oligonucelotide in Nile tilapia against Streptococcus iniae infection and identification of upregulated genes

    Science.gov (United States)

    At two days post treatment, a CpG oligodeoxynucleotide (CpG 120818-9A) offered Nile tilapia (Oreochromis niloticus L.) significant (PStreptococcus iniae infection, with relative percent survival up to 63%. To understand the molecular mechanisms involved in the protective im...

  5. Identification of multiple genes and their expression profiles in four strains of Oreochromis spp. in response to Streptococcus iniae.

    Science.gov (United States)

    Wang, R; Chen, M; Li, C; Li, L-P; Gan, X; Huang, J; Lei, A-Y; Xu, Z-H; Liang, W-W

    2013-02-01

    Two subtractive complementary DNA libraries were constructed from Nile tilapia Oreochromis niloticus vaccinated with formalin-killed Streptococcus iniae cells, and a further two constructed from O. niloticus infected with S. iniae. Of the 68 distinct expressed sequence tag (EST) contigs and singletons, 45 and 13 EST shared high similarities with genes of known and unknown functions, respectively. Ten EST contigs and singletons had no significant similarity to any sequences. Five putative immune-relevant genes, β2m, α-ha, mmp9, pgrn and cxcr4, were selected for quantitative reverse-transcription polymerase chain reaction in four strains of Oreochromis spp.: genetically improved farmed tilapia (O. niloticus), Oreochromis aureus, O. niloticus and O. niloticus×O. aureus, with different disease resistance following infection with S. iniae. pgrn was up-regulated more significantly in disease-resistant strains than in the susceptible. α-ha was markedly down-regulated, and no significant differences in the expression level of β2m were detected. A negative correlation was observed between the expression of mmp9 and that of cxcr4. The results provide insight into the molecular response of O. niloticus to S. iniae infection. PMID:23398064

  6. Variation in the immunoglobulin levels in turbot (Scophthalmus maximus) after vaccination with Streptococcus iniae

    Institute of Scientific and Technical Information of China (English)

    ZHAN Wenbin; LIU Hongming; XING Jing; SHENG Xiuzhen; TANG Xiaoqian

    2009-01-01

    A pathogenic bacterium (S636), identified as Streptococcus iniae, was isolated from turbot (Scophthalmus maximus) in 2005. We immunized turbot with formalin-killed S. iniae four times (on days 1, 14, 21, and 28) by intraperitoneal inoculation. After each vaccination, we obtained serum samples and isolated the lymphocytes from the peripheral blood, spleen, pronephros, and mesonephros. We measured surface Ig-positive (sIg+) lymphocytes and serum antibody levels from these organs using flow cytometry and enzyme-linked immunosorbent assay (ELISA), respectively, using monoclonal antibodies against turbot immunoglobulin. We confirmed that the antibody reacted with both the surface and plasma Ig by confocal laser scanning microscopy and electron microscopy. The percentage of sIg+ in the lymphocytes increased following each successive vaccination. The mean percentage increased from 31.96% (control) to 37.49%, 38.36%, 42.9%, and 51.63% in the peripheral blood; from 27.09% to 36.63%, 36.81%, 39.28%, and 46.0% in the spleen; from 22.2% to 28.99%, 29.21%, 32.83%, and 41.58% in pronephros; and from 18.12% to 22.17%, 22.45%, 25.69%, and 31.68% in the mesonephros. The ELISA results were consistent with these results. Both the total and specific antibody levels increased with each vaccination. The mean OD value of the specific antibody assay increased from 0.094, to 0.269, 0.283, 0.333, and 0.421; for total antibody the mean OD value increased from 0.133, to 0.292, 0.323, 0.413, and 0.527.

  7. Variation in the immunoglobulin levels in turbot ( Scophthalmus maximus) after vaccination with Streptococcus iniae

    Science.gov (United States)

    Zhan, Wenbin; Liu, Hongming; Xing, Jing; Sheng, Xiuzhen; Tang, Xiaoqian

    2009-09-01

    A pathogenic bacterium (S636), identified as Streptococcus iniae, was isolated from turbot ( Scophthalmus maximus) in 2005. We immunized turbot with formalin-killed S. iniae four times (on days 1, 14, 21, and 28) by intraperitoneal inoculation. After each vaccination, we obtained serum samples and isolated the lymphocytes from the peripheral blood, spleen, pronephros, and mesonephros. We measured surface Ig-positive (sIg+) lymphocytes and serum antibody levels from these organs using flow cytometry and enzyme-linked immunosorbent assay (ELISA), respectively, using monoclonal antibodies against turbot immunoglobulin. We confirmed that the antibody reacted with both the surface and plasma Ig by confocal laser scanning microscopy and electron microscopy. The percentage of sIg+ in the lymphocytes increased following each successive vaccination. The mean percentage increased from 31.96% (control) to 37.49%, 38.36%, 42.9%, and 51.63% in the peripheral blood; from 27.09% to 36.63%, 36.81%, 39.28%, and 46.0% in the spleen; from 22.2% to 28.99%, 29.21%, 32.83%, and 41.58% in pronephros; and from 18.12% to 22.17%, 22.45%, 25.69%, and 31.68% in the mesonephros. The ELISA results were consistent with these results. Both the total and specific antibody levels increased with each vaccination. The mean OD value of the specific antibody assay increased from 0.094, to 0.269, 0.283, 0.333, and 0.421; for total antibody the mean OD value increased from 0.133, to 0.292, 0.323, 0.413, and 0.527.

  8. Valinia INIA, a New Early Storage Onion Variety for Central South Chile Valinia INIA, una Nueva Variedad de Cebolla de Guarda Precoz para la Zona Centro Sur de Chile

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    María Inés González A

    2012-03-01

    Full Text Available In Central South Chile, storage onion varieties (Allium cepa L. sold domestically usually begin the maturation process in March, which implies that a large proportion of bulbs do not mature adequately and do not resist prolonged storage. This situation led the Quilamapu vegetable program of the Instituto de Investigaciones Agropecuarias INIA to select an early maturing onion variety. This breeding method involved a recurrent selection with free plant crossing that began in 1994 on a population of 2000 plants of the 'Valenciana' commercial variety. Plants were first selected when they reached physiological maturity (tops down before 28 February, and then for shape, size, storage capacity, and single-centered bulbs. The result of the program was 'Valinia INIA', a type of Valenciana onion variety: early maturing, medium-sized bulb, spherical, yellow, and resistant to sprouting during storage. From the 2001-2002 to the 2008-2009 season, variety assays were carried out to compare 'Valinia INIA' with commercial cultivars, i.e., 'Sintética 14', 'Grano de Oro', and 'Valenciana Cobra'. 'Valinia INIA' precocity exceeded the other varieties in all seasons with a mean of 1.5 wk ahead of 'Sintética 14', which is the earliest commercial variety and more than 2 wk ahead of the other two. Its yield is similar to 'Valenciana Cobra' and higher than the other two varieties. Its storage capacity is similar to 'Grano de Oro' and 'Sintética 14', but greater than 'Valenciana Cobra'.En la zona centro sur de Chile es habitual que las variedades de cebolla (Allium cepa L. de guarda que se comercializan en el pais inicien el proceso de maduración preferentemente en marzo, lo que conlleva a que una gran proporción de bulbos no madure adecuadamente, no resistiendo el almacenaje por un tiempo prolongado. Esta situación llevó al programa de hortalizas del Instituto de Investigaciones Agropecuarias INIA Quilamapu a la selección de una variedad de cebolla de madurez

  9. COMBARBALA-INIA, UN CULTIVAR PRECOZ DE HUALPUTRA (Medicago polymorpha L.) PARA AREAS DE SECANO MEDITERRANEO. Combarbalá-INIA, an early flowering cultivar of burr medic (Medicago polymorpha L.) for Mediterranean dryland areas.

    OpenAIRE

    Alejandro del Pozo L.; Carlos Ovalle M; Julia Avendaño R.; Teresa Aravena; M. Elena Díaz

    2001-01-01

    Combarbalá-INIA es un nuevo cultivar de hualputra (Medicago polymorpha L.), seleccionado entre un gran número de accesiones colectadas en Chile, entre La Serena (29º 55’ lat. Sur) y Temuco (38º 47’ lat. Sur). Es una leguminosa forrajera anual, precoz (71 - 83 días desde emergencia a primera flor), apta para áreas de secano de la zona mediterránea árida, semiárida y subhúmeda, es decir, entre la IV y VI Regiones de Chile. Presenta un hábito de crecimiento postrado, flores de color amarillo, y ...

  10. Influencia de tres densidades de siembra y cuatro niveles de fertilización en la variedad de maíz INIA 612-Maselba

    OpenAIRE

    Walker Cubas Pérez; Christian Córdova Díaz

    2013-01-01

    El experimento se realizó el año 2008, en el Campo Experimental San Miguel de la EEA. San Roque del INIA - Loreto, con el objetivo de determinar el efecto de diferentes densidades de siembra y niveles de fertilización nitrogenada en el rendimiento de grano de la variedad de maíz amarillo duro, INIA 612 – MASELBA; en condiciones de suelos aluviales de restinga inundable. Se evaluaron tres densidades de siembra, 0,80m x 0,50m con 2 plantas por sitio (50 000 plantas/ha), 0,8m x 0,60m con 3 plant...

  11. Differential transcriptomic response in the spleen and head kidney following vaccination and infection of Asian seabass with Streptococcus iniae.

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    Junhui Jiang

    Full Text Available Vaccination is an important strategy in the protection of aquaculture species from major diseases. However, we still do not have a good understanding of the mechanisms underlying vaccine-induced disease resistance. This is further complicated by the presence of several lymphoid organs that play different roles when mounting an immune response. In this study, we attempt to elucidate some of these mechanisms using a microarray-based approach. Asian seabass (Lates calcarifer were vaccinated against Streptococcus iniae and the transcriptomic changes within the spleen and head kidney at one and seven days post-vaccination were profiled. We subsequently challenged the seabass at three weeks post-vaccination with live S. iniae and similarly profiled the transcriptomes of the two organs after the challenge. We found that vaccination induced an early, but transient transcriptomic change in the spleens and a delayed response in the head kidneys, which became more similar to one another compared to un-vaccinated ones. When challenged with the pathogen, the spleen, but not the head kidneys, responded transcriptomically at 25-29 hours post-challenge. A unique set of genes, in particular those involved in the activation of NF-κB signaling, was up-regulated in the vaccinated spleens upon pathogen challenge but not in the un-vaccinated spleens. A semi-quantitative PCR detection of S. iniae using metagenomic DNA extracted from the water containing the seabass also revealed that vaccination resulted in reduction of pathogen shedding. This result indicated that vaccination not only led to a successful immune defense against the infection, but also reduced the chances for horizontal transmission of the pathogen. In conclusion, we have provided a transcriptomic analysis of how the teleost spleen and head kidneys responded to vaccination and subsequent infection. The different responses from the two organs are suggestive of their unique roles in establishing a

  12. Molecular cloning, expression and the adjuvant effects of interleukin-8 of channel catfish (Ictalurus Punctatus) against Streptococcus iniae

    Science.gov (United States)

    Wang, Erlong; Wang, Jun; Long, Bo; Wang, Kaiyu; He, Yang; Yang, Qian; Chen, Defang; Geng, Yi; Huang, Xiaoli; Ouyang, Ping; Lai, Weimin

    2016-01-01

    Interleukin-8 (IL-8) as an important cytokine involving in inflammatory and immune response, has been studied as effective adjuvants for vaccines in mammals. However, there are fewer reports about the characterization and adjuvant effects of IL-8 in fish. In this study, cloning and sequence analysis of IL-8 coding region of channel catfish (Ictalurus punctatus) were conducted, mature IL-8(rtIL-8) was expressed and evaluated for its adjuvant effects on the immunoprotection of subunit vaccine encoding α-enolase (rENO) of Streptococcus iniae from several aspects in channel catfish. The results showed co-vaccination of rENO with rtIL-8 enhanced immune responses including humoral and cellular immunity, with higher relative percent survival(RPS,71.4%) compared with the moderate RPS of rENO alone(50%) against S. iniae infection at 4 week post vaccination. While rtIL-8 failed to maintain long-lasting immune protection, only with RPS of 26.67% in rENO + rtIL-8-vaccinated fish compared with that of rENO alone(20%) at 8 week, signifying that IL-8 hold promise for use as potential immunopotentiator in vaccines against bacterial infections in fish, whereas it is insufficient to extend the immunoprotection for long time, and further studies are required to understand the mechanisms of IL-8 used as an adjuvant and seek for more effective way to strengthen the adjuvanticity of IL-8. PMID:27373470

  13. Molecular cloning, expression and the adjuvant effects of interleukin-8 of channel catfish (Ictalurus Punctatus) against Streptococcus iniae.

    Science.gov (United States)

    Wang, Erlong; Wang, Jun; Long, Bo; Wang, Kaiyu; He, Yang; Yang, Qian; Chen, Defang; Geng, Yi; Huang, Xiaoli; Ouyang, Ping; Lai, Weimin

    2016-01-01

    Interleukin-8 (IL-8) as an important cytokine involving in inflammatory and immune response, has been studied as effective adjuvants for vaccines in mammals. However, there are fewer reports about the characterization and adjuvant effects of IL-8 in fish. In this study, cloning and sequence analysis of IL-8 coding region of channel catfish (Ictalurus punctatus) were conducted, mature IL-8(rtIL-8) was expressed and evaluated for its adjuvant effects on the immunoprotection of subunit vaccine encoding α-enolase (rENO) of Streptococcus iniae from several aspects in channel catfish. The results showed co-vaccination of rENO with rtIL-8 enhanced immune responses including humoral and cellular immunity, with higher relative percent survival(RPS,71.4%) compared with the moderate RPS of rENO alone(50%) against S. iniae infection at 4 week post vaccination. While rtIL-8 failed to maintain long-lasting immune protection, only with RPS of 26.67% in rENO + rtIL-8-vaccinated fish compared with that of rENO alone(20%) at 8 week, signifying that IL-8 hold promise for use as potential immunopotentiator in vaccines against bacterial infections in fish, whereas it is insufficient to extend the immunoprotection for long time, and further studies are required to understand the mechanisms of IL-8 used as an adjuvant and seek for more effective way to strengthen the adjuvanticity of IL-8. PMID:27373470

  14. Effects of dietary levels of vitamin A on growth, hematology, immune response and resistance of Nile tilapia (Oreochromis niloticus) to Streptococcus iniae

    Science.gov (United States)

    This study was conducted to evaluate the effect of supplemental levels of vitamin A (0, 2,500, 5,000, 10,000, and 20,000 IU/kg diet) on the growth performance, hematology, immune response and resistance of Nile tilapia, Oreochromis niloticus to Streptococcus iniae challenge. Each diet was fed to Nil...

  15. Growth performance, immune response and resistance to Streptococcus iniae of Nile tilapia fed diets containing various levels of vitamins C and E

    Science.gov (United States)

    The effect of dietary levels of vitamin C (0, 100, 2,000 mg/kg), E (0, 50, 500 mg/kg), and their interaction on growth performance, immune response and resistance of Nile tilapia, Oreochromis niloticus to Sterptococcus iniae challenge were evaluated. Each diet was fed to Nile tilapia in triplicate a...

  16. Growth Response and Resistance to Streptococcus iniae of Nile Tilapia Oreochromis niloticus Fed Diets Containing Distiller’s Dried Grains with Solubles

    Science.gov (United States)

    This study was conducted to evaluate the effect of dietary levels of distiller’s dried grains with solubles (DDGS) on growth performance, body composition, hematology, immune response and resistance of Nile tilapia to Streptococcus iniae challenge. Five isocaloric diets containing DDGS at levels of ...

  17. Phenotypic and genotypic heterogeneity among Streptococcus iniae isolates recovered from cultured and wild fish in North America, Central America and the Caribbean Islands

    Science.gov (United States)

    Streptococcus iniae, the etiological agent of streptococcosis in fish, is an important pathogen of cultured and wild fish worldwide. During the last decade outbreaks of streptococcosis have occurred in a wide range of cultured and wild fish in the Americas and Caribbean islands. To gain a better und...

  18. Fitness cost, gyrB mutation, and absence of phosphotransferase system fructose specific IIABC component in novobiocin-resistant Streptococcus iniae vaccine strain ISNO

    Science.gov (United States)

    To understand the fitness cost of novobiocin-resistance in an attenuated Streptococcus iniae vaccine strain ISNO compared to its virulent parent strain ISET0901, cell proliferation rate of the two strains were compared to each other. Our results revealed that the cell proliferation rates of ISNO wer...

  19. Growth performance and resistance to Streptococcus iniae of juvenile Nile tilapia (Oreochromis niloticus) fed diets supplemented with GroBiotic - A and Brewtech Dried Brewers Yeast

    Science.gov (United States)

    This study was conducted to evaluate the effect of dietary levels of Brewtech® dried brewers yeast (BY) and GroBiotic®-A (GB) on growth performance, proximate body composition, immune response and resistance of juvenile Nile tilapia to Streptococcus iniae challenge. A practical basal (control) diet ...

  20. Effectiveness of Aquaflor (50% florfenicol) administered in feed to control mortality associated with Streptococcus iniae in tilapia at a commercial tilapia production facility

    Science.gov (United States)

    Gaikowski, Mark P.; Schleis, Susan M.; Leis, Eric; Lasee, Becky A.; Endris, Richard G.

    2014-01-01

    The efficacy of Aquaflor (florfenicol; FFC) to control mortality caused by Streptococcus iniae in tilapia was evaluated under field conditions. The trial was initiated following presumptive diagnosis of S. iniae infection in a mixed group of fingerling (mean, 4.5 g) Nile Tilapia Oreochromis niloticus and a hybrid of Nile Tilapia×Blue Tilapia O. aureus. Diagnoses included mortality in source tank; examination of clinical signs and presence or absence of gram-positive cocci in brain, and collection of samples for microbiological review and disease confirmation of 60 moribund fish. Following presumptive diagnosis, tilapia (83/tank) were randomly transferred to each of 20 test tanks receiving the same water as the source tank (test tank water was not reused). Tilapia were offered either nonmedicated control feed or FFC-medicated feed (FFC at 15 mg/kg body weight/d; 10 tanks per regimen) for 10 consecutive days followed by a 14-d observation period during which only the nonmedicated control feed was offered. Streptococcus iniae was presumptively identified during pretreatment necropsy and confirmed by polymerase chain reaction assay; S. iniae was confirmed in samples taken during the dosing period but was not detected during the postdosing period. The FFC disk diffusion zone of inhibition ranged from 29 to 32 mm, while the minimum inhibitory concentration of FFC ranged from 2 to 4 μg/mL for the S. iniae isolates collected. Survival of tilapia assigned to the FFC-dose group was significantly greater at 14 d posttreatment than that of the nonmedicated controls. The odds of tilapia assigned to the FFC-dose group surviving to the end of the postdosing period were 1.34 times the odds of survival of tilapia assigned to the nonmedicated control group. There were no clinically apparent adverse effects associated with the administration of FFC-medicated feed in this study.

  1. Millán-INIA, New Variety of High Quality Early Spring Bread Wheat for Irrigated Soils in Central-Southern Chile Millán-INIA, Nueva Variedad de Trigo Harinero Precoz de Primavera de Alta Calidad para Suelos de Riego de la Zona Centro Sur de Chile

    OpenAIRE

    Iván Matus; Ricardo Madariaga; Claudio Jobet; Javier Zúñiga; Christian Alfaro

    2011-01-01

    Millán-INIA is a variety of spring bread wheat (Triticum aestivum L.) originating from a cross carried out in the Wheat Plant Breeding Project of the Instituto de Investigaciones Agropecuarias (INIA), in the Centro Regional de Investigación Quilamapu in 1995. This is a spring wheat variety with an early to medium-early head emergence and upright growth habit in the seedling stage. The adult plant is low to medium height and varies between 75 and 90 cm, with a mean of 85 cm. The spike is white...

  2. Multiplex PCR for simultaneous detection of Streptococcus agalactiae, Streptococcus iniae and Lactococcus garvieae: a case of S. agalactiae infection in cultured Nile tilapia (Oreochromis niloticus) and red tilapia (Oreochromis niloticus x Oreochromis mossambicus)

    OpenAIRE

    Chutima Tantikitti; Naraid Suanyuk; Akkarawit Itsaro

    2012-01-01

    A multiplex PCR (m-PCR) technique was developed for simultaneous detection of the causative agents responsible forstreptococcosis of cultured fish in Thailand i.e., Streptococcus agalactiae, Streptococcus iniae, and Lactococcus garvieae.The study on the sensitivity of the technique indicated that the minimum detected DNA concentration was 9.76, 39.06, and19.53 pg for S. agalactiae, S. iniae and L. garvieae, respectively. Detection of streptococcosis in healthy and diseased Niletilapia (Oreoch...

  3. O Desenvolvimento do Turismo Náutico e a sua Ligação com a Observação do Boto-Cinza (sotalia guianensis na Região de Cananéia, Litoral Sul do Estado de São Paulo

    Directory of Open Access Journals (Sweden)

    Gislaine de Fátima Filla

    2010-07-01

    Full Text Available Resumo O boto-cinza é hoje um grande atrativo e gerador de renda para a região estuarina de Cananéia. O crescimento da infra-estrutura turística desta região parece estar intimamente relacionado com o crescimento do interesse em observar estes animais na natureza. Assim, um levantamento das atuais estruturas de alojamento e capacidade náutica da região foi feito nos meses de verão de 2007, através de visitas aos estabelecimentos e entrevistas com pessoas atuantes no turismo náutico. Ficou evidente que as estruturas de alojamento e o uso das embarcações nas três ilhas do estuário variam de acordo com as diferentes categorias de turistas que freqüentam esta região e com o objetivo de suas viagens. Ainda, foi possível notar que a organização em associações e parcerias garantiu um crescimento maior da atividade de observação do boto-cinza, a qual deve ser acompanhada com cuidado para que seja desenvolvida de forma otimizada, conservando os animais e seu habitat natural. Palavras-chaves: boto-cinza; sotalia guianensis; turismo náutico; Cananéia Abstract The estuarine dolphin (sotalia guianensis is a big attraction and money maker for the Cananéia estuary region. The increased tourist infrastructure of the region seems to be directly related to the growing interest to observe these animals in loco. Therefore, a survey was done to asses the present boarding and nautical capacity of the region during the summer months of 2007, by means of visits to the establishments and interviews with the people working with nautical tourism. It was evident that the boarding structures and the use of ships on the three islands of the estuary vary according to the different type of tourists that visit the area and their reasons for the trip. Even so, it was possible to note that organized associations and partnerships guaranteed a larger growth and activity of estuarine dolphin observation, which must be carefully followed to ensure proper

  4. Millán-INIA, New Variety of High Quality Early Spring Bread Wheat for Irrigated Soils in Central-Southern Chile Millán-INIA, Nueva Variedad de Trigo Harinero Precoz de Primavera de Alta Calidad para Suelos de Riego de la Zona Centro Sur de Chile

    Directory of Open Access Journals (Sweden)

    Iván Matus

    2011-06-01

    Full Text Available Millán-INIA is a variety of spring bread wheat (Triticum aestivum L. originating from a cross carried out in the Wheat Plant Breeding Project of the Instituto de Investigaciones Agropecuarias (INIA, in the Centro Regional de Investigación Quilamapu in 1995. This is a spring wheat variety with an early to medium-early head emergence and upright growth habit in the seedling stage. The adult plant is low to medium height and varies between 75 and 90 cm, with a mean of 85 cm. The spike is white with long awns along its full length. The grain is large-sized, white, and vitreous. The weight of 1000 grains varies between 50 and 59 g. It was sown in mid-August at the Santa Rosa Experimental Station (36°31’ S; 71°54’ W, Chillán. Head emergence occurred 88 to 89 d after sowing, which is 2 to 3 d after Ciko-INIA. On average, Millán-INIA reached a yield similar to that of the var. Ciko-INIA. This line stands out for its good resistance to disease, high protein content (11.5% mean, high sedimentation value, and high W value.Millán-INIA es un trigo harinero (Triticum aestivum L. de primavera que proviene de un cruzamiento efectuado en 1995 en el Proyecto de Fitomejoramiento de Trigo del Instituto de Investigaciones Agropecuarias INIA, en el Centro Regional de Investigación Quilamapu. Es un trigo de hábito primaveral, de época de espigadura mediana a precoz, con hábito de crecimiento erecto al estado de plántula. La altura de la planta adulta se considera mediana a baja, y varía entre 75 y 90 cm, con un promedio de 85 cm. La espiga es de color blanco y de barbas largas y presentes en toda su extensión. El grano es de color blanco y aspecto vítreo y de tamaño grande, con un peso de los 1000 granos que varía entre 50 y 59 g. Sembrado a mediados de agosto en el Campo Experimental Santa Rosa (36°31’ S; 71°54’ O, Chillán, la emisión de espigas ocurre 88 a 89 días después de la siembra, entre 2 y 3 días después que Ciko-INIA. Como

  5. Construction of a Streptococcus iniae sortase A mutant and evaluation of its potential as an attenuated modified live vaccine in Nile tilapia (Oreochromis niloticus).

    Science.gov (United States)

    Wang, J; Zou, L L; Li, A X

    2014-10-01

    Streptococcus iniae is a major Gram-positive aquatic pathogen, which causes invasive diseases in cultured fish worldwide. The identification of potential virulence determinants of streptococcal infections will help to understand and control this disease, but only a few have been confirmed in S. iniae. Sortase A (srtA) is the key enzyme that anchors pre-mature cell wall-attached proteins to peptidoglycan and it can affect the correct positioning of surface proteins, as well as the course of Gram-positive bacterial infection, thereby making it a potential target in the study of virulence factors and disease control. In this study, the 759 bp srtA gene was cloned from pathogenic S. iniae TBY-1 strain and the mutant strain TBY-1ΔsrtA was constructed via allelic exchange mutagenesis. We found that srtA shares high similarities with sortase A from other Streptococcus spp. Direct survival rate assay and challenge experiments were performed, which showed that the mutant strain TBY-1ΔsrtA had a lower survival capacity in healthy tilapia blood and it was less virulent than the wild type strain in tilapia, thereby indicating that the deletion of sortase A affects the virulence and infectious capacity of S. iniae. The mutant strain TBY-1ΔsrtA was used as a live vaccine, which was administered via intraperitoneal injection, and it provided the relative percent survival value of 95.5% in Nile tilapia, thereby demonstrating its high potential as an effective attenuated live vaccine candidate. PMID:25090938

  6. Host-derived probiotics Enterococcus casseliflavus improves resistance against Streptococcus iniae infection in rainbow trout (Oncorhynchus mykiss) via immunomodulation.

    Science.gov (United States)

    Safari, Reza; Adel, Milad; Lazado, Carlo C; Caipang, Christopher Marlowe A; Dadar, Maryam

    2016-05-01

    The present study evaluated the benefits of dietary administration of host-derived candidate probiotics Enterococcus casseliflavus in juvenile rainbow trout Oncorhynchus mykiss. Experimental diets were prepared by incorporating the microorganisms in the basal feed at 3 inclusion levels (i.e. 10(7) CFU g(-1) of feed [T1], 10(8) CFU g(-1) of feed [T2], 10(9) CFU g(-1) of feed [T3]). The probiotic feeds were administered for 8 weeks, with a group fed with the basal diet serving as control. The effects on growth performance, gut health, innate immunity and disease resistance were evaluated. Results showed that growth performance parameters were significantly improved in T2 and T3 groups. Activities of digestive enzymes such as trypsin and lipase were significantly higher in these two groups as well. Gut micro-ecology was influenced by probiotic feeding as shown by the significant increase in intestinal lactic acid bacteria and total viable aerobic counts in T2 and T3. Humoral immunity was impacted by dietary probiotics as total serum protein and albumin were significantly elevated in T3. The levels of serum IgM significantly increased in all probiotic fed groups at week 8; with the T3 group registering the highest increment. Respiratory burst activity of blood leukocytes were significantly improved in T2 and T3. Hematological profiling further revealed that neutrophil counts significantly increased in all probiotic fed groups. Challenge test showed that probiotic feeding significantly improved host resistance to Streptococcus iniae infection, specifically in T2 and T3 where a considerable modulation of immune responses was observed. Taken together, this study demonstrated E. casseliflavus as a potential probiotics for rainbow trout with the capability of improving growth performance and enhancing disease resistance by immunomodulation. PMID:26997202

  7. Seasonal and daily patterns of group size, cohesion and activity of the estuarine dolphin, Sotalia guianensis (P.J. van Bénéden (Cetacea, Delphinidae, in southern Brazil Padrões sazonais e diários do tamanho de grupo, coesão e atividade do boto-cinza, Sotalia guianensis (P.J. van Bénéden (Cetacea, Delphinidae, no sul do Brasil

    Directory of Open Access Journals (Sweden)

    Fábio G. Daura-Jorge

    2005-12-01

    Full Text Available The resident population of estuarine dolphin, Sotalia guianensis (P.J. van Bénéden, 1864, in Norte Bay, Santa Catarina, southern Brazil, was studied from 2000 to 2003. Systematic one-day cruises were undertaken aboard a 5 m sail boat equipped with an outboard motor. Applying the focal-group method, information such as geographical position, predominant behavioural patterns, group size, cohesion index and the presence of calves, were registered at every five-minute interval, totalling 198 hours of direct observation of the dolphins. The estuarine dolphin used preferential areas for each behavioural pattern. The mean group size was approximately 29 individuals, and the presence of calves occurred throughout the entire year, but with a significant increase in the warm seasons. The frequency of behavioural patterns and group size varied according to season and time of day, and were strongly associated. The frequency of moving behaviours increased in the cold seasons and along the day. The seasonal variation in the moving behaviours suggest the requirement of a larger area in the cold seasons, probably related to seasonal fluctuations in the abundance of dominant prey items. Higher group sizes were observed while dolphins were foraging. The cohesion index also varied according to the behaviour. Our results showed that group organization and behavioural patterns of this estuarine dolphin population are probably linked to daily and seasonal environment cycles, and also possibly to the condition of being at the furthest southern limit of distribution of the species.A população residente de boto-cinza, Sotalia guianensis (P.J. van Bénéden, 1864, da Baía Norte, Santa Catarina, sul do Brasil, foi estudada de 2000 a 2003. Foram realizados cruzeiros sistemáticos de um dia, a bordo de um veleiro de 5 m equipado com motor de popa. Utilizando o método de grupo-focal, informações como posição geográfica, padrão comportamental predominante, tamanho

  8. Dietary Aloe vera improves plasma lipid profile, antioxidant, and hepatoprotective enzyme activities in GIFT-tilapia (Oreochromis niloticus) after Streptococcus iniae challenge.

    Science.gov (United States)

    Gabriel, Ndakalimwe Naftal; Qiang, Jun; Ma, Xin Yu; He, Jie; Xu, Pao; Liu, Kai

    2015-10-01

    The current study investigated the effects of dietary Aloe vera on plasma lipid profile status, antioxidant, and hepatoprotective enzyme activities of GIFT-tilapia juveniles under Streptococcus iniae challenge. Five dietary groups were designed including a control and 100 % Aloe powder incorporated into a tilapia feed at 0.5, 1, 2, and 4 %/kg feed, which were administered for 8 weeks. Fish fed dietary Aloe at 4 %/kg feed significantly reduced in total cholesterol, while triacylglycerol reduced (P Aloe/kg feed compared to unsupplemented ones. High-density lipoprotein was significantly elevated in fish fed 0.5 and 1 % Aloe/kg feed compared to unsupplemented ones, and no significant changes (P > 0.05) were noted in low-density lipoprotein among test groups. Furthermore, high activities of superoxide dismutase, catalase, and glutathione peroxide in liver tissues were observed in Aloe-supplemented fish compared to unsupplemented ones, before and after S. iniae challenge (7.7 × 10(6) CFU cells/mL). Variations were also noted in malondialdehyde activity throughout the trial, but no significant difference (P > 0.05) was observed between groups. Meanwhile, Aloe-supplemented fish reduced serum aspartate and alanine aminotransferase (AST and ALT) activities before and after challenge. Based on the second-order polynomial regression analysis, dietary Aloe inclusion levels less than or equal to 1.88, 1.86, and 2.79 %/kg feed were determined to be suitable in improving plasma lipid profile status, antioxidant, and hepatoprotective enzyme activities in GIFT-tilapia in this study, respectively. Thus, A. vera extracts may be recommended as a tilapia feed supplement to enhance fish antioxidant and hepatoprotective capacities, especially during disease outbreaks.

  9. Streptococcus iniae infection in cultured tilapia, sea bass, and barramundi in Guangdong%广东省养殖罗非鱼、海鲈、尖吻鲈海豚链球菌感染调查

    Institute of Scientific and Technical Information of China (English)

    张俊; 周素明; 李安兴

    2012-01-01

    利用细菌分离培养方法结合特异PCR技术,对广东省珠三角地区养殖罗非鱼(Oreochromis spp.)、海鲈(Lateolabrax japonicus)及尖吻鲈(Lates calcarifer)的海豚链球菌(Streptococcus iniae)感染情况进行了周年调查.每月固定时间在特定养殖区域采集目标鱼的脑、肝、脾、肾和肌肉等组织,并对其进行海豚链球菌的细菌分离培养鉴定.仅从已经患病的尖吻鲈中分离到3株链球菌,经生理生化鉴定和16S rDNA测序确定为海豚链球菌.利用海豚链球菌特异PCR技术对上述养殖鱼类不同组织进行检测,发现罗非鱼、海鲈、尖吻鲈的海豚链球菌感染率分别为30.21%、23.53%、14.55%,其中罗非鱼脑和肌肉的感染率明显较其他组织高(P<0.05),分别为20.65%和23.75%;海鲈的脑部和肌肉感染率也较其他组织高(P<0.05),分别为12.1%和10%;而尖吻鲈各组织感染率没有较大差异(P>0.05).另外,研究结果还表明采集样本的海豚链球菌感染率随着其体长的增加而呈现下降趋势.%Streptococcus iniae infects a number of cultured fish species, causing heavy economic loss in Guangdong province. We evaluated the prevalence of infection with S. Iniae in tilapia {Oreochromis spp.), sea bass {Lateolabrax japonicus), and barramundi (Lates calcarifex) using a standard microbiological assay and specific PCR. Using PCR, we detected S. Iniae in all three species (30.21%, 23.53%, and 14.55% of individuals, respectively). However, S. Iniae was only isolated from three barramundi using the traditional microbiological assay. S. Iniae was detected in all tissues, including brain, liver, spleen, kidney, and muscle. The brain and muscle were more susceptible to S. Iniae than the other tissues in tilapia (infection rate: 20.65% and 23.75%, respectively) and sea bass (infection rate: 12.1% and 10%, respectively). There was no difference in the susceptibility of the tissues in barramundi. Moreover, our results

  10. Dietary Aloe vera supplementation on growth performance, some haemato-biochemical parameters and disease resistance against Streptococcus iniae in tilapia (GIFT).

    Science.gov (United States)

    Gabriel, Ndakalimwe Naftal; Qiang, Jun; He, Jie; Ma, Xin Yu; Kpundeh, Mathew D; Xu, Pao

    2015-06-01

    This study investigated effects of dietary Aloe vera on growth performance, some haemato-biochemical parameters and disease resistance against Streptococcus iniae in tilapia (GIFT). Five groups were designed including a basal diet (control) and 100% A. vera powder incorporated in fish feed at 0.5% 1%, 2%, and 4%/kg feed, which were administered for 8 weeks. Fish fed 0.5%, 1%, and 2% A. vera supplemented diet significantly improved (p vera diet at 1% and 2%/kg feed. Feed efficiency ratio, feed conversion ratio, and hepatosomatic index were significantly enhanced in 4% A. vera supplemented fish over unsupplemented ones (p vera supplemented fish showed a significant increase (p vera supplemented fish showed a decrease (p vera diet at 2% and 4% A. vera/kg feed than those fed unsupplemented diet. Unchallenged fish fed 0.5%, 1%, and 2% A. vera showed significantly higher values (p vera supplemented diet. There was a significant increase (p vera unsupplemented fish and those supplemented with A. vera diet at 1%/kg feed increased significantly (p vera supplemented diet maintained higher values at all experimental stages among groups. There was a significant correlation (p Aloe had no significant effect (p > 0.05) on the survival of the fish when compared to the control; no mortality was recorded in challenge trial. Overall, our results indicated that dietary aloe supplementation could improve growth, feed utilization, and haemato-biochemical parameters of cultured tilapia.

  11. Morphologic characterization and evaluation of the national collection of germoplasm of camu camu Myrciaria dubia (H.B.K Mc Vaugh, of the INIA Loreto-Peru

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    Sixto Imán Correa

    2011-12-01

    Full Text Available The aim of this study was to characterize and evaluate morphologically the national collection of germplasm INIA camu camu made up of 43 accessions collected in the Loreto region, for conservation and use. The results show that the germplasm of camu camu has high variability and three types of plant architecture (column, intermediate and branched. The flowers are hermaphrodite or androgynous and have herkogamy empowering the outcrossing or cross-pollination. The color of the fruit depends on the state of maturation of the same, varies from green to red-violet, the number of seeds per fruit varies from 1 to 4, the number of fruits per kg is 90 to 148. Twenty one accessions were found with yields greater than 15 kg per plant and 16 accessions of over 2000 mg of ascorbic acid /100 g of pulp. In addition, we identified 10 promising accessions by fruit yield and ascorbic acid contents. Through Principal Component Analysis was determined that the variables are positively associated with the fruit yield, plant height and stem diameter. The dendrogram derived from cluster analysis allowed the identification of three groups based on fruit yield, the first group consists of 22 accessions whose fruit yields are between 2.29 and 11.97 kg / plant, the second group 16 accessions that have yields 16.97 to 30.35 kg of / plant, and the third group consists of 5 accessions from 36.52 to 39.24 yields kg/plant.

  12. Research on Screening of Antagonistic Bacteria against Streptococcus iniae in Tilapia Cultural Water%拮抗罗非鱼养殖水体中海豚链球菌功能性微生物的筛选

    Institute of Scientific and Technical Information of China (English)

    瞿建宏; 陈辉; 吴伟

    2014-01-01

    Eleven strains of antagonistic bacteria against Streptococcus iniae were isolated from the preserved bacteria with the ability of purification of stimulant aquaculture wastewater isolated from the tilapia cultural water. The most compatible strains were chosen for the growth curves, co-agglutination assay and ability of cleanse effects in the aquaculture wastewater. The result showed:7 isolated strains had more advantages in RI than Streptococcus iniae, and shorter doubling time were found in L09017, C0909 and N0906 indicating the growth advantages in the intestine of Streptococcus iniae;3 (L0914, C0909, N0906) of 7 strains had co-agglutination effects with Streptococcus iniae and their co-agglutination ratios were 3.03%, 4.11%and 1.85%, respectively;all 7 strains had the ability of cleanse effects on the aquaculture wastewater, but C0909, L0914 had fewer advantages in degrading ammonia-nitrogen than N0906. In conclusion, N0906 was more moderate to be used as the bio-control bacterium against Streptococcus iniae in tilapia and had good development and research values.%以罗非鱼链球菌病的病原--海豚链球菌(Streptococcus iniae)为目标菌株,研究了多株分离自罗非鱼养殖水体具有净化水质功能的微生物对其的拮抗作用。结果表明:通过初筛获得了11株对海豚链球菌具有拮抗功能的微生物菌株,其中7株拮抗效果较为显著,分别属于芽孢杆菌(B0903, B0910, B0916)、乳杆菌(L0914, L0917)、假丝酵母(C0909)和诺卡氏菌(N0906)。对这7株菌株从生长优势、共凝集率、净化水质能力等三方面进行进一步的筛选,发现7株菌株的生长系数均大于海豚链球菌,L0917、C0909、N0906的倍增时间显著低于海豚链球菌,且L0914、C0909、N0906能与海豚链球菌产生共凝集作用,共凝集率分别为3.03%、4.11%和1.85%。7株菌都具有一定的净化水质的能力,但C0909、L0914对氨氮的去除率显著低于菌株N0906。研

  13. Effects of Nitrogen on Productivity, Grain Quality, and Optimal Nitrogen Rates in Winter Wheat cv. Kumpa-INIA in Andisols of Southern Chile Efecto del Nitrógeno en Productividad, Calidad del Grano y Dosis Óptimas de Nitrógeno en Trigo Invernal cv. Kumpa-INIA en Andisoles del Sur de Chile

    Directory of Open Access Journals (Sweden)

    Ricardo Campillo

    2010-03-01

    Full Text Available Nitrogen is one of the main inputs of the winter wheat crop (Triticum aestivum L. in southern Chile. Nitrogen efficient management is basic to optimizing its utilization while decreasing pollution risks and operational costs. Crop response and N use efficiency (NUE, defined as the ratio of yield to mineral N supply, regardless of source are important for evaluating N requirements of winter wheat, and reaching maximum and economic yields. The objective of this study was to determine the effect of N rate on grain yield, calculate the N rate that maximizes yield, and estimate the optimal grain yield rate and quality of high-yielding winter wheat cv. Kumpa-INIA. Five annual N rates were evaluated in a randomized complete block design during two successive winter wheat cropping seasons on a Vilcún series soil of the Pachic Melanudands family (Andisol in La Araucania Region, Chile, and subjected to intensive annual crop rotation. Significant effects (P ≤ 0.01 of N rate on grain yield and quality were found. The optimal physical N rate (OPR in both seasons ranged from 290 to 339 kg ha-1, whereas optimal economic N rate (OER ranged from 248 to 274 kg ha-1, with yields between 10.2 and 10.1 t ha-1. Nitrogen use efficiency associated to OER was high in both seasons (36.9 and 41.2 kg grain kg-1 N and fluctuated in similar ranges. Nitrogen rate increased hectoliter weight and grain protein, but decreased NUE.El N es uno de los principales insumos del cultivo de trigo (Triticum aestivum L. en Chile. Su manejo eficiente optimiza las dosis, disminuye los riesgos de contaminación y los costos de producción. La respuesta del cultivo y eficiencia de uso de N (NUE, definida como la razón de rendimiento y suministro de N mineral, independientemente de la fuente son importantes para evaluar los requerimientos de N y alcanzar rendimientos máximos y económicos. El objetivo de este estudio fue determinar el efecto del N en la producción y las dosis que

  14. 卵形鲳鲹感染无乳链球菌与海豚链球菌的研究%Pathogenic bacteria Streptococcus agalactiae and S. iniae in diseased ovate pompano Trachinotus ovatus

    Institute of Scientific and Technical Information of China (English)

    黄婷; 梁万文; 陈明; 李莉萍; 王瑞; 张彬; 甘西; 罗洪林; 陈福艳; 蔡小辉; 杨传萍

    2014-01-01

    于2011年8月对广西北海疑似链球菌感染的卵形鲳鲹Trachinotus ovatus进行病原分离鉴定,经人工感染试验,确定分离的两株病原菌(编号为TSG002和TSG004)为卵形鲳鲹的致病菌,然后对两株病原菌进行形态学、生理生化特征、16S rRNA基因序列和二重PCR快速检测综合鉴定,构建系统进化树,最后对两株菌进行药敏分析。根据染色形态特征和二重PCR快速检测,初步鉴定菌株TSG002和TSG004分别为无乳链球菌和海豚链球菌,菌株TSG002和TSG004的16S rRNA基因序列(登录号分别为KF826095和KF826094)分别与无乳链球菌 ATCC13813 strain JCM 5671基因(登录号 NR040821.1)和海豚链球菌ATCC29178基因(登录号AF335572.1)的相似性最高,均达99%。药敏试验结果表明,两株菌对头孢曲松、头孢呋辛、头孢哌酮、恩诺沙星、氧氟沙星、阿莫西林、多粘菌素B、头孢噻吩和头孢他啶均敏感。研究表明,感染无乳链球菌和海豚链球菌的卵形鲳鲹可发病死亡,在病鱼中同时分离到两种链球菌尚属首例。%Two bacterial strains TSG002 and TSG004 were isolated from the diseased ovate pompano Trachinotus ovatus collected in Beihai,Guangxi Province,China in August of 2011,and were confirmed to be the pathogens of the diseased ovate pompano by animal challenge test, and by morphology, physiological and biochemical examina-tion and 16S rRNA genetic analysis. In addition,the sensitivity of both strains to twenty-two antibiotics were stud-ied. The results showed that strains TSG002 and TSG004 were similar to bacteria Streptococcus agalactiae and S. in-iae, based on the morphology, physiology and biochemistry examinations. The phylogenetic analysis revealed that the l6 S rRNA sequence of strains TSG002 ( KF826095 ) and TSG004 ( KF826094 ) has high similarity ( 99%) to S. agalactiae (NR040821. 1) and S. iniae (AF335572. 1) in GenBank. Phylogenetic tree of Streptococcus spp. built

  15. Isolation and identification of pathogenetic Streptococcus iniae from Selenotoca multi fasciata%银鼓鱼病原菌(海豚链球菌)的分离与鉴定

    Institute of Scientific and Technical Information of China (English)

    罗璋; 许杰; 韩进刚; 徐赟霞; 马文婷; 冯守明

    2012-01-01

    One strain Ygl was isolated from the kidney of diseased Selenotoca multifasciata. The healthy Selenotoca multifasciata could be infected through artificial infection by these bacteria. The symptoms in artificially infected fish were similar to those infected naturally. The characters of re-isolated strains were the same as the initially isolated strain, which was identified as Streptococcus iniae by the means of ATB system,morphological, physiological and biochemical tests, and 16S rRNA sequence analyses. The pathogens were sensitive to the following antibiotics: enrofloxacin, oxytetracycline, rox-ithromycin, doxycycline, tetracycline, levofloxacin.%从患病濒死的银鼓鱼(Selenotoca multi fasciata)肾脏组织中分离到Ygl菌株,用该菌株对健康银鼓鱼进行人工感染,可使其致病,发病症状与自然病鱼症状一致,再分离菌株的各种特性与原分离菌株相同.通过ATB系统和菌体常规形态特征、生理生化反应指标测定以及16S rRNA测序分析等综合鉴定,Yg1菌株为海豚链球菌(Streptococcus iniae).药敏试验结果表明,Yg1菌株对恩诺沙星、土霉素、罗红霉素、强力霉素、四环素、左氟沙星等抗生素较为敏感.

  16. Studies on antibacterial effects of 10 kinds of Chinese medicinal herbs and four combinations on pathogenic Streptococcus iniae from tilapia in Vitro%10种中草药及4种组方对罗非鱼致病性海豚链球菌体外抑菌试验

    Institute of Scientific and Technical Information of China (English)

    张彬; 黄婷; 陈福艳; 陈明; 梁万文

    2011-01-01

    The antibacterial effects of 10 Chinese herbal medicines including Coptidis rhizome, Rheum qffcinale, Radix scutellariae, Phellodendron chinense, etc. To Streptococcus iniae were detected through disc agar diffusion method ( punch method), and the minimal inhibitory concentration and the minimum bactericidal concentration of four kind Chinese herbs which had significant inhibitory effects to S. Iniae were measured by microdilution procedure. The results showed that 10 Chinese herbal medicine had inhibitory effects on 5. Iniae to different degree, among which four kinds of Chinese herbs including C. Rhizome, R. Scutellariae, R. Qffcinale and P. Chinense could effectively inhibit the growth of pathogenic S. Iniae, furthermore the inhibitory effect of C. Rhizome was the best, and the average diameter was (25.67 ± 0. 58) mm. Meanwhile , the experiment revealed that when used in combination, the antibacterial actions of 4 kinds of herb medicines were characterized as below: P. Chinense with C. Rhizome, R. Qffcinale with R. Scutellariae had a enhanced effect, R. Qffcinale with C. Rhizome remained unchanged, but P. Chinense with R. Scutellariae, P. Chinense with R. Qffcinale, Radix scutellariae with Coptidis rhizome depressed.%采用琼脂扩散法(打孔法)研究了黄连等10种中草药及4种组方对尼罗罗非鱼(Oreochromis niloticus)致病性海豚链球菌(Streptococcus iniae)的抑制作用,并通过二倍稀释法测定抑菌作用较强的中草药对海豚链球菌的抑菌和杀菌浓度.结果显示:10种中草药对海豚链球菌均有不同程度的抑制作用,其中黄连、黄芩、大黄和黄柏的抑菌作用较强,而以黄连的抑菌效果最佳,抑菌圈平均直径达(25.67±0.58) mm.黄柏与黄连、大黄与黄芩联用抗菌作用增强,大黄与黄连联用不变,而黄柏与黄芩、黄柏与大黄、黄芩与黄连联用抑菌能力降低.

  17. 杂交鳢海豚链球菌病病原分离鉴定及药敏试验%Isolation and identification of pathogen Streptococcus iniae in diseased hybrid snakehead (Channa argus♂× Channamaculata♀)

    Institute of Scientific and Technical Information of China (English)

    陈言峰; 周爱国; 邹青; 陈冠锋; 陈建酬; 张继平; 张辉华

    2015-01-01

    【目的】对广东省中山市某养殖场的患病濒死杂交鳢进行病原分离鉴定及药敏试验,旨在确定病因,为今后生产中有效防控该病提供参考。【方法】从濒死杂交鳢体内分离致病菌,通过其生理生化特征与16S rRNA基因序列分析进行菌株鉴定,并采用纸片扩散法进行药敏试验。【结果】从患病濒死的杂交鳢体内分离获得两株优势菌(ZS2013711-1和ZS2013711-2),根据其生理生化鉴定结果与16S rRNA基因序列分析结果,可鉴定为海豚链球菌(Streptococcusiniae)。两株分离菌株对杂交鳢的半致死剂量(LD50)分别为2.7×105和6.7×105 CFU/g。药敏试验结果显示,该致病菌对头孢噻肟、头孢拉定、头孢唑啉、林可霉素等抗生素高度敏感。【结论】杂交鳢是海豚链球菌的一种新发现宿主,生产中可选用头孢噻肟、头孢拉定、头孢唑啉、林可霉素等药物进行防治。%Objective]The pathogens from diseased hybrid snakehead (Channa argus♂×Channa maculata♀) in an aquafarm in Zhongshan City of Guangdong Province were isolated and identified and antibiotic sensitivity test was per-formed in order to determine the causes and provide references for preventing and controlling this disease effectively.[Method]Pathogenic bacteria were isolated from moribund hybrid snakehead , and they were identified through physio-logical and biochemical characteristics test and 16S rRNA sequence analysis. Furthermore , antibiotic sensitivity test was performed through Kirby-Bauer disk diffusion method. [Result]Two dominant bacterias (ZS2013711-1, ZS2013711-2) were isolated from moribund hybrid snakehead , and they were identified to be Streptococcus iniae according to their physiological and biochemical characteristics and 16S rRNA sequence analysis. The half lethal dose values for the iso-lates to healthy hybrid snakehead were 2.7 ×105 and 6.7 ×105 CFU/g, respectively. The results of

  18. Preliminary study on immune effect of Streptococcus iniae inactivated vaccine in Oreochromis niloticus%海豚链球菌灭活疫苗对罗非鱼免疫效果的初步研究

    Institute of Scientific and Technical Information of China (English)

    张俊; 黄木珍; 李安兴

    2011-01-01

    Formalin killed vaccines with/without white oil adjuvant were prepared with TBY-1 strain of Streptococcus iniae isolated from freshwater fish. The efficacy of the vaccines were determined in Oreochromis niloticus by bath immersion and intraperitoneal (IP) injection successively. The relative percent survival (RPS) of each immune group was statisticsed. Moreover, the length and weight of the tilapias were measured and analyzed by SPSS. The results showed that the RPS of the fish immuned with bath immersion and then immunized intraperitoneally once with none-adjuvant vaccine and white oil adjuvant were 50. 50% and 71. 10% respectively, and the antibody agglutination titer were 1:4. 8 and 1: 128 respectively. The RPS of fish immuned with bath immersion and then immunized intraperitoneally twice with none-adjuvant vaccine and white oil adjuvant were 82. 50% and 58. 80% respectively, and the antibody agglutination titer were 1∶6.4 and 1∶27.4 re spectively.%将海豚链球菌(Streptococcus iniae)淡水分离株TBY-1菌株灭活后制备成无佐剂和添加白油佐剂的疫苗,利用浸泡和腹腔注射复合免疫方式对罗非鱼(Oreochromis niloticus)进行免疫,比较其相对保护率及血清中抗体凝集效价,以评价佐剂的存在与否及免疫次数对海豚链球菌灭活疫苗的免疫效果的影响,同时还对实验前后罗非鱼的体长体重进行测量和统计,并用SPSS软件进行分析.结果显示,用无佐剂疫苗进行首次浸泡免疫后,再分别用无佐剂疫苗及白油佐剂疫苗注射免疫1次的相对保护率分别为50.50%和71.10%,血清中抗体凝集效价分别为1∶4.8和1∶128;而注射免疫2次的相对保护率分别为82.50%和58.80%,其血清凝集效价分别为1∶6.4和1∶27.4.

  19. Pellet feed adsorbed with the recombinant Lactococcus lactis BFE920 expressing SiMA antigen induced strong recall vaccine effects against Streptococcus iniae infection in olive flounder (Paralichthys olivaceus).

    Science.gov (United States)

    Kim, Daniel; Beck, Bo Ram; Lee, Sun Min; Jeon, Jongsu; Lee, Dong Wook; Lee, Jae Il; Song, Seong Kyu

    2016-08-01

    The aim of this study was to develop a fish feed vaccine that provides effective disease prevention and convenient application. A lactic acid bacterium (LAB), Lactococcus lactis BFE920, was modified to express the SiMA antigen, a membrane protein of Streptococcus iniae. The antigen was engineered to be expressed under the nisin promoter, which is induced by nisin produced naturally by the host LAB. Various sizes (40 ± 3.5 g, 80 ± 2.1 g, and 221 ± 2.4 g) of olive flounder (Paralichthys olivaceus) were vaccinated by feeding the extruded pellet feed, onto which the SiMA-expressing L. lactis BFE920 (1.0 × 10(7) CFU/g) was adsorbed. Vaccine-treated feed was administered twice a day for 1 week, and priming and boosting were performed with a 1-week interval in between. The vaccinated fish had significantly elevated levels of antigen-specific serum antibodies and T cell marker mRNAs: CD4-1, CD4-2, and CD8a. In addition, the feed vaccine significantly induced T cell effector functions, such as the production of IFN-γ and activation of the transcription factor that induces its expression, T-bet. When the flounder were challenged by intraperitoneal infection and bath immersion with S. iniae, the vaccinated fish showed 84% and 82% relative percent survival (RPS), respectively. Furthermore, similar protective effects were confirmed even 3 months after vaccination in a field study (n = 4800), indicating that this feed vaccine elicited prolonged duration of immunopotency. In addition, the vaccinated flounder gained 21% more weight and required 16% less feed to gain a unit of body weight compared to the control group. The data clearly demonstrate that the L. lactis BFE920-SiMA feed vaccine has strong protective effects, induces prolonged vaccine efficacy, and has probiotic effects. In addition, this LAB-based fish feed vaccine can be easily used to target many different pathogens of diverse fish species. PMID:27302864

  20. Vitamin C content in fruits of camu camu Myrciaria dubia (H.B.K Mc Vaugh, in four states of maturation, coming from the Collection of Germoplasma of the INIA Loreto, Peru

    Directory of Open Access Journals (Sweden)

    Sixto Imán Correa

    2011-09-01

    Full Text Available The objective of the present work was to determine the vitamin content C in different parts from the fruit; pulp, rind and pulp more rind, in four states of maturation: green, mature green, mature and over mature. The fruits were obtained from the Collection of Germoplasma of the Agrarian Experimental Station San Roque of the INIA Loreto, Peru. The technique used for the determination of vitamin content C was Liquid High Chromatography Performance (HPLC with phase column reverse. The results indicate that the greater vitamin contents C are in the rind and the states of over mature and mature. And vitamin C contained according to maturation states adjusts to a curve of cubical regression, as much for pulp, rind and pulp more rind with 87%, 90% and 98 % of effectiveness; respectively. When moving from a state of maturity to another, the vitamin C content is increased in 515.43 mg in each 100 g of rind sample. The obtained results indicate that camu camu is a potential vitamin C source, located mainly in the rind of the fruit.

  1. 环介导等温扩增联合横向流动试纸条可视化检测海豚链球菌方法的建立%Visual Detection of Streptococcus iniae Based on Loop- mediated Isothermal Amplification Combined with a Lateral Flow Dipstick

    Institute of Scientific and Technical Information of China (English)

    王瑞娜; 周前进; 陈炯

    2014-01-01

    海豚链球菌(Streptococcus iniae)是一种革兰氏阳性球菌,呈β溶血,可感染多种淡水和海洋鱼类。本研究利用环介导等温扩增技术(loop-mediated isothermal amplification, LAMP)进行核酸扩增,通过横向流动试纸条方法(lateral flow dipstick, LFD)实现检测,建立了一种可应用于海豚链球菌快速检测的LAMP-LFD技术。该技术以海豚链球菌促旋酶B亚单位(gyrase subunit B, gyrB)基因为检测靶标,设计3对引物进行由生物素标记的LAMP扩增反应,产物经异硫氰酸荧光素(fluorescein isothiocyanate, FITC)标记的探针杂交后,在LFD上完成检测。经优化的核酸扩增最适条件为65℃反应30 min,在此条件下,阳性扩增起始时间与模板浓度之间呈典型的线性相关性。从核酸扩增反应开始到LFD显色,整个检测时程只需40 min左右,比常规PCR技术缩短约2 h。LAMP-LFD能特异性地检出海豚链球菌,针对病原纯培养物的检测灵敏度为8.70×101 cfu/mL,是LAMP检测的10倍、常规PCR检测的100倍。以灵敏度浓度(8.70×101 cfu/mL)的海豚链球菌基因组DNA为模板进行的LAMP-LFD结果显示该方法具有良好的重复性。针对人工污染花鲈(Lateolabrax japonicus)肝组织的检测灵敏度为4.35×103 cfu/mL,同样为常规PCR检测方法的100倍。利用本方法可成功从患病花鲈的组织样品中检测出海豚链球菌,检测结果与常规的细菌分离鉴定方法结果一致。因此,利用LAMP-LFD能特异、准确、高效地检测出海豚链球菌,而且操作简单、费用低、耗时短,有望成为海豚链球菌的常规检测方法。%Streptococcus iniae is a species belonging to Gram-positive coccus and produces beta hemolysis, which can infect a broad range of freshwater and marine fish species and lead to seriously economic losses in the aquaculture industry worldwide. Thus, a diagnostic method for rapid and accurate detection of S. iniae was

  2. Cloning,identification and molecular characteristics analysis of simA gene of a Streptococcus iniae strain DGX07 from channel catfish%斑点叉尾(鮰)源海豚链球菌DGX07株simA基因克隆、鉴定及分子特性分析

    Institute of Scientific and Technical Information of China (English)

    汪开毓; 王均; 肖丹; 陈德芳; 黄锦炉; 黄凌远; 付希; 王浩丞

    2012-01-01

    以Primer p14(5’-GATCAAGTCC-3’)为引物对分离自患病斑点叉尾(鮰)海豚链球菌强毒株DGX07进行RAPD分析.同时参照GenBank中海豚链球菌simA基因序列设计特异性引物,以DGX07基因组DNA为模板,扩增出约1 500 bp的simA基因,并将其克隆到pMD19-T载体上,之后对重组质粒进行PCR和双酶切(BamH Ⅰ+Xho Ⅰ)鉴定,鉴定正确后送测序公司测序.RAPD分析结果显示,DGX07和标准菌株均能扩增出750 bp大小的条带.通过生物信息学软件对测序结果分析显示,simA基因全长1 566bp,由521个氨基酸组成,与海豚链球菌simA和simB亲缘性达100%,存在1个由41个氨基酸组成的信号肽,具有Bap31和Gram_pos_anchor两个超家族的保守结构域;具有与蛋白翻译后修饰功能相关的磷酸化位点22个和N-糖基化位点2个,编码多肽链中亲水区大于疏水区,是一种膜外蛋白,并具有多个抗原优势位点区域.密码子偏爱性分析表明,斑点叉尾(鮰)源海豚链球菌simA基因密码子使用频率差异较大,密码子偏爱性与酵母较为接近.获得GenBank登录号为JF330100.%The RAPD analysis of Streptococcus iniae virulent strain DGX07 isolated from channel catfish (Ictalurus punctatus)v/as conducted by PCR with Primer pl4(5'-GATCAAGTCC-3'). The simA gene of DGX07 was amplified by PCR with specific primers and cloned into pMD19-T vector. The positive plasmid was selected and identified by PCR and digestion of double restriction enzyme (BamH I + Xho I ). Molecular characterization analysis of simA gene was performed by bioinformatics tools. The results showed that S. Iniae simA amino acid sequence was highly conservative and with 100% homology to the simA and simB of S. Iniae isolated. The polypeptide contained a signal peptide comprising 41 amino acids and Bap31 and Gram _ pos _ anchor functional domains. The polypeptide had some important sites related to post-translational modification, including 22 phosphorylation sites and 2 N

  3. 海豚链球菌感染对不同品系罗非鱼血液生化指标和肝脏HSP70 mRNA表达的影响%Studies on blood biochemical indices and expression of hepatic HSP70 mRNA of different tilapia strains artificially challenged with Streptococcus iniae

    Institute of Scientific and Technical Information of China (English)

    强俊; 杨弘; 王辉; 徐跑; 柒壮林; 何杰

    2012-01-01

    In order to establish the superiority, four different tilapia strains (GIFT tilapia, New GIFT tilapia, Egypt Nile tilapia and Red tilapia) were compared in the study. At day 100 tilapias in the treatment group were intraperitoneally injected with Streptococcus iniae suspension (2.95×108/mL) to evaluate disease resistance of four different tilapia strains against S. iniae in terms of the serum biochemical parameters and hepatic HSP70 mRNA expression before and after infection. Another set of twenty fish taken from each tank was challenged with S. iniae using the same method as above, and the cumulative mortality of 4 strains was recorded at different times post infection. The results showed that GIFT tilapia and New GIFT tilapia were more sensitive to S. iniae at 96 h after infection, cumulative mortalities were 36.67% and 38.33% respectively. Egypt Nile tilapia was less sensitive to pathogen and no death occurred during the experiment. The levels of serum cortisol (COR) and glucose (GLU) and expression levels of HSP70 mRNA in GIFT tilapia, New GIFT tilapia and Red tilapia strains were highly significant(P<0.05), and the activities of glutamic-pyruvic transaminase (ALT), glutamic-oxaloacetic transaminase (AST)and lysozyme (LSZ) also increased, while serum alkaline phosphatase (AKP), triglyceride and cholesterol levels were significantly lower than those prior to infection (P<0.05). By utilizing the energy from resolved hepatin and lipid, the synthesis of hepatic HSP70 mRNA and levels of LSZ and globulin in Egypt Nile tilapia were improved, and thus the non-specific immunity strengthened. It is suggested that in the course of tilapia breeding, effective combination of the disease resistance with growth be conducted so as to guarantee sustainable development of the tilapia industry.%以吉富罗非鱼、新吉富罗非鱼、埃及尼罗罗非鱼和红罗非鱼为研究对象,饲养100 d后,进行海豚链球菌(2.95×108 CFU/mL)感染试验,分析攻毒前后各品

  4. 氨氮胁迫下罗非鱼(GIFT Oreochromis niloticus)机体免疫力的变化及其对海豚链球菌易感性的影响%The immune response of GIFT Oreochromis niloticus and its susceptibility to Streptococcus iniae under stress in different ammonia

    Institute of Scientific and Technical Information of China (English)

    陈家长; 臧学磊; 胡庚东; 瞿建宏; 范立民; 宋超

    2011-01-01

    研究了不同作用时间(0、24、48、96和120 h)下不同质量浓度氨氮(对照组、1、3、5和10 mg·L-1)对罗非鱼(GIFT Oreochromis niloticus)血清SOD、AKP、溶菌酶和补体C3活性的影响,以及不同质量浓度氨氮(对照组、1、3、5和10 mg·L-1)胁迫下罗非鱼对海豚链球菌的易感性.结果显示:在1.0 mg·L-1氨氮质量浓度下,罗非鱼血清SOD活性在24 h受到诱导;在3.0、5.0和10.0 mg·L-1氨氮质量浓度下,罗非鱼血清SOD活性均受到抑制,最大抑制率分别达18.85%、23.47%和38.46%.在1.0 mg·L-1氨氮质量浓度下,罗非鱼血清AKP活性在0~96 h呈先降低后恢复的趋势;5.0和10.0 mg·L-1实验组的血清AKP活性在24~120 h则一直受到抑制.1.0 mg·L-1实验组的罗非鱼血清溶菌酶活性在24~120 h一直无显著变化,3.0、5.0和10.0mg·L-1实验组的血清溶菌酶活性随着氨氮质量浓度的升高而降低.1.0 mg·L-1实验组的罗非鱼血清补体C3活性在各个采样时间下均大于对照组,而5.0和10.0 mg·L-1实验组补体C3活性始终受到抑制.罗非鱼对海豚链球菌的易感性实验研究显示,置于3.0、5.0和10.0 mg-L-1的罗非鱼的累积死亡率分别为43.33%、46.67%和63.33%,显著高于对照组和1.0 mg·L-1实验组,表明氨氮质量浓度超过3.0 mg·L-1能够增加罗非鱼对海豚链球菌的易感性.本实验认为,超过3 mg·L-1的氨氮质量浓度胁迫能够显著降低罗非鱼的免疫力,增加对海豚链球菌的易感性.因此在罗非鱼健康养殖中,调控并降低氨氮质量浓度非常重要.%The experiment, including the susceptible test of tilapia exposed to Streptococcus iniae and the test on effect of immune-related enzymes (superoxide dismutaso, SOD; lysozyme; alkaline phosphates, AKP and supplement C3) to tilapia under different ammonia, was carried out to reveal the relationship between ammonia and its stress effect on the susceptible to tilapia and the immunity to fish exposed to

  5. The evaluation of the risk for floodings on the territory of Botoșani municipal town

    Directory of Open Access Journals (Sweden)

    Delia Nicoleta VIERU

    2010-04-01

    Full Text Available The county of Botosani is not placed in an area of high risk for flooding, because most of the lands within the build-up area are situated on the ledge. Because of the increase ofthe population, some socio-economical objectives have been located in the high risk area on the basis of the ledge; it’s necessary that the real insurance and that provided by therules could be established, thereby, the following measures need to be established: the achievement of field works against floods, the gad of the buildings exposed to the majorrisk. The real insurance of the buildings located on the four effluents (located at the base of the ledge, varies between 5 – 10 per cent, in comparison to 1 per cent as it is foreseen bythe rules. The dams built up on these valeys, made up of local materials and generally used for fishy needs, have large uploaders calculated under 1 per cent error, which might lead to dangerous waves of flood, in case of river burst. The possible damages have both direct (material and human losses and indirect results (traffic jams, coming up to normal conditions, and the price of the operative measures for preventing human victims The estimation of the damages to certain flood is inconclusive, the most important damage being the one afferent to the main class of the objective, without mentioning the ecological damages, as in case of extreme humidity, epidemic dangers, quick reproduction of mosquitos etc.

  6. Parasitism affects vaccine efficacy against Streptococcus iniae in Nile tilapia

    Science.gov (United States)

    Tilapia culture worldwide is estimated to be US$ 5 billion and is important to domestic and global food security. Parasites and bacteria co-occur in both extensive and intensive production of tilapia. The effect of parasitism on vaccine performance in fish is little studied. The objective of this ...

  7. Riqueza de mamíferos de grande e médio porte do Parque Nacional da Serra do Divisor (Acre, Brasil The richness of the large and medium-sized mammals of the Serra do Divisor National Park (Acre, Brazil

    Directory of Open Access Journals (Sweden)

    Armando Muniz Calouro

    1999-01-01

    Full Text Available The objective of the present study was to characterize the richness (number of species of large and medium-sized mammals, and the antropic threats in the Parque Nacional da Serra do Divisor (PNSD. In 31 days data were collected through direct observations or evidences (bones, hairs, vocalizations and tracks along pre-existing trails distributed in different types of vegetation. Wild mammals captured by local people were also considered. They were found 44 species of terrestrial mammals (with the exception of small mammals and bats and two species of cetaceans, representing 73% of the total predicted, according to the literature and information of local dwellers were registered in PNSD. They exist in the area two species classified by IUCN (1996 as "Endangered" [Cacajao calvus rubicundus (I. Geoffroy, 1806 and Priodontes maximus (Kerr, 1792] and five as "Vulnerable" [Ateles chamek (Humboldt, 1812, Callimico goeldii (Thomas, 1904, Lagothrix lagotricha poeppigii (Humboldt, 1812, Myrmecophaga tridactyla Linnaeus, 1758 and Inia geoffrensis (Blainville, 1817]. Given that subsistence and commercial hunting are common in the PNSD, mammals more affected by hunting [Ateles chamek (Humboldt, 1812, Lagothrix lagotricha (Humboldt, 1812, Tayassu pecari (Link, 1795] were observed only in the more remote areas such as Serra do Divisor, Rio Moa. In comparison with others areas, the results indicate that PNSD has high richness of the mammals, with special attention to the 14 primates species registered.

  8. VACCINES TO PREVENT Streptococcus iniae AND S. agalactiae DISEASE IN NILE TILAPIA Oreochromis niloticus

    Science.gov (United States)

    Minimizing the effects of disease is crucial to prevent mortality, morbidity, and to promote optimal growth and feed conversion in sustained culture of warm-water fish in fresh, estuarine and marine waters. The control of diseases has been dependent on the use of therapeutics since the inception of...

  9. Considerations on FEM modeling in analyzing buckling and plastic collapse of a detection control; Boto panel no zakutsu sosei hokai kaiseki ni okeru FEM model ka ni kansuru kosatsu

    Energy Technology Data Exchange (ETDEWEB)

    Yao, T.; Fujikubo, M.; Yanagihara, D.; Irisawa, M. [Hiroshima University, Hiroshima (Japan). Faculty of Engineering

    1997-10-01

    Buckling and plastic collapse of upper decks and bottom outer plates of a hull results directly in longitudinal bending collapse of the hull. Therefore, discussions were given on analysis for pressure destruction strength of a detection control panel which assumes an upper deck and a bottom outer plate. Pressure destruction behavior of the panting panel is a complex phenomenon accompanying non-linearity and geometrical non-linearity of the materials. Its whole phenomenon may be analyzed by using the finite element method (FEM) as a principle, but the analysis is not efficient. Therefore, considerations were given in relation to modeling when using the FEM. The considerations were given on a panel attached with flat steel panting members with respect to the modeling scope which considers the buckling mode according to the aspect ratio of the panel partitioned by the deflection control members. If the local buckling mode of the panel is an even number wave mode in the longitudinal direction, a triple span model is required. A modeling scope for a case of being subjected to water pressure and in-plane compression was considered on a panel attached with angle-type steel members having non-symmetric cross section. In this case, a triple bay model is more preferable to reproduce the behavior under water pressure loading. 1 ref., 6 figs.

  10. Host-derived probiotics Enterococcus casseliflavus improves resistance against Streptococcus iniae infection in rainbow trout (Oncorhynchus mykiss) via immunomodulation

    DEFF Research Database (Denmark)

    Safari, Reza; Adel, Milad; Lazado, Carlo Cabacang;

    2016-01-01

    that growth performance parameters were significantly improved in T2 and T3 groups. Activities of digestive enzymes such as trypsin and lipase were significantly higher in these two groups as well. Gut micro-ecology was influenced by probiotic feeding as shown by the significant increase in intestinal lactic...... CFU g-1 of feed [T1], 108 CFU g-1 of feed [T2], 109 CFU g-1 of feed [T3]). The probiotic feeds were administered for 8 weeks, with a group fed with the basal diet serving as control. The effects on growth performance, gut health, innate immunity and disease resistance were evaluated.Results showed...

  11. Growth Performance and Resistance to Streptococcus iniae of Nile Tilapia, Oreochromis niloticus Fed Various Dietary Levels of Thiamin

    Science.gov (United States)

    Thiamin or vitamin B1 functions in all cells as the coenzyme thiamin pyrophosphate that involves in the oxidative decarboxylation of an alpha-keto acids and the transketolase reactions in the pentose phosphate pathway. Thiamin has been demonstrated to be essential in diets of fish, and quantitative ...

  12. Dietary nucleotide supplementation enhances immune responses and survival to Streptococcus iniae in hybrid tilapia fed diet containing low fish meal

    Directory of Open Access Journals (Sweden)

    Shi-Yen Shiau

    2015-11-01

    Full Text Available A feeding trial was conducted to evaluate the effects of nucleotide (NT supplementation in diet on immune responses and disease resistance of juvenile hybrid tilapia, Oreochromis niloticus × Oreochromis aureus. Nucleotide was added at 0, 120, 240, 360, 480 and 600 mg NT/kg to low fish meal (6% and high soybean meal (56% basal diet for a total of 6 experimental diets. Each diet was fed to triplicate groups of tilapia (initial body weight 0.15 ± 0.005 g in a recirculated freshwater rearing system for 10 weeks. Head kidney leukocyte superoxide anion production ratio was higher (P 80% were observed in fish fed diets supplemented with NT than fish fed the NT unsupplemented control diet (56.7%. These results suggest that nucleotides supplemented at 120–240 mg NT/kg in diet enhances immune responses and survival of tilapia fed low fish meal and high soybean meal diet.

  13. Molecular cloning, expression and the adjuvant effects of interleukin-8 of channel catfish (Ictalurus Punctatus) against Streptococcus iniae

    OpenAIRE

    Erlong Wang; Jun Wang; Bo Long; Kaiyu Wang; Yang He; Qian Yang; Defang Chen; Yi Geng; Xiaoli Huang; Ping Ouyang; Weimin Lai

    2016-01-01

    Interleukin-8 (IL-8) as an important cytokine involving in inflammatory and immune response, has been studied as effective adjuvants for vaccines in mammals. However, there are fewer reports about the characterization and adjuvant effects of IL-8 in fish. In this study, cloning and sequence analysis of IL-8 coding region of channel catfish (Ictalurus punctatus) were conducted, mature IL-8(rtIL-8) was expressed and evaluated for its adjuvant effects on the immunoprotection of subunit vaccine e...

  14. Morfologia do sincrânio do boto-cinza, Sotalia guianensis (P.J. van Bénéden (Cetacea, Delphinidae Syncranial morphology of the estuarine dolphin Sotalia guianensis (P.J. van Bénéden (Cetacea, Delphinidae

    Directory of Open Access Journals (Sweden)

    Paulo C. Simões-Lopes

    2006-09-01

    Full Text Available Apresenta-se uma descrição da morfologia comparada do sincrânio de Sotalia guianensis (P.J. van Bénéden, 1864, incluindo suas variações ontogenéticas e intra-específicas. As descrições foram baseadas em 51 crânios completos ou parciais depositados na coleção do laboratório de Mamíferos Aquáticos, do Departamento de Ecologia e Zoologia, CCB, Universidade Federal de Santa Catarina. O sincrânio é bastante homogêneo e manifesta sua assimetria na morfologia dos ossos, passagens nasais e forames. O rostro é proporcionalmente estreito em relação aos outros Delphinidae do mesmo porte. A região temporal é circular em indivíduos jovens, mas se torna oval nos adultos onde se forma uma crista temporal evidente. O forame elíptico do periótico está presente tanto em indivíduos jovens quanto adultos. Os nasais são bipartidos e podem ocorrer ossos suturais entre os frontais. O processo hamular dos pterigóides é bastante inflado e fenestrado. A sínfise mandibular alcança de 19 de 19-23% do comprimento do dentário. Cada pré-maxilar e maxilar possui de 30-35 dentes e cada dentário de 30-38 dentes.Morphological and comparative description of the syncranium of Sotalia guianensis (P.J. van Bénéden, 1864, including ontogenic aspects and intraspecific variations are presented. The descriptions were based on the examination of 51 complete or partial skulls, kept at the Marine Mammals Lab. Collection from the Departamento de Ecologia e Zoologia, CCB, at the Federal University of Santa Catarina. The sincranium is very homogeneous and manifests its asymmetry in the morphology of the bones, nasal passages and foramina. The rostrum is proportionally narrow in relation to other Delphinidae of the same size. The temporal region is circular in young specimens, but it becomes oval in the adults were it forms a well marked temporal crest. The elliptical foramen of the periotic bone is present both in young and adult individuals. The nasals are bipartite and one may find sutural bones between the frontals. The hamular process of the pterygoids is inflated and somewhat fenestrated. The mandibular symphysis covers from 19-23% of the dentary length. Each premaxilla and maxilla has 30-35 teeth and each dentary exhibits 30-38 teeth.

  15. OPPORTUNITIES AND RISKS OF GLOBALIZATION IN RURAL AREAS. CASE STUDY: BOTOSANI COUNTY

    Directory of Open Access Journals (Sweden)

    Maria-Simona Cuciureanu

    2014-04-01

    Full Text Available The global economy plays an important role in the development of a region or a county/district as it promotes the exploitation of resources and space in a logical and rational manner. The trend of global economic uniformity allows opportunities and risks to Botoșani County since globalization involves economic development and rising living standards, but loss of cultural values, traditions and customs. The area of study currently confronts with socio-economic and demographic changes that may be addressed by globalization, but at the same time spatial development according to global standards will cause the loss of Botoșani County’s authenticity.

  16. Comportamento do boto-cinza, Sotalia guianensis (Van Bénédén (Cetacea, Delphinidae na presença de barcos de turismo na Praia de Pipa, Rio Grande do Norte, Brasil Behavior of estuarine dolphin, Sotalia guianensis (Van Bénédén (Cetacea, Delphinidae in the presence of tourist boats in Pipa Beach, Rio Grande do Norte, Brazil

    Directory of Open Access Journals (Sweden)

    Érico Santos-Jr

    2006-09-01

    Full Text Available A crescente indústria do turismo tem se estendido ao ambiente aquático, deste modo tornando os mamíferos aquáticos suscetíveis a interações com humanos. O turismo de observação de golfinhos na praia de Pipa litoral sul do Rio Grande do Norte, tem se desenvolvido aumentando o número de barcos de observação de golfinhos operando nesta área. No presente estudo alterações comportamentais do Sotalia guianensis (Van Bénédén, 1864 causado pela presença destes barcos foi verificada. Registros comportamentais de S. guianensis foram compilados de um ponto fixo do alto de uma falésia próxima à área de estudo. As observações foram realizadas quando não havia barcos, na presença de barcos e após a saída dos barcos. Os resultados mostraram que o tipo de aproximação praticado pelos barcos de observação de golfinhos tinha maior influência no comportamento dos animais, especialmente em grupos com filhotes. Os impactos de curto prazo foram mais discretos, no entanto, novos métodos devem ser aplicados no sentido de verificar impactos de longo prazo no comportamento dos golfinhos.The ever-increasing tourist industry has extended to the aquatic environments, thus making the aquatic mammals susceptible to interactions with humans. Dolphin watching tourism has developed to a great extent in the Pipa Beach, Rio Grande do Norte, consequently the number of dolphin-watching boats operating in this area has increased. In the present study the behavioral alterations of Sotalia guianensis (Van Bénédén, 1864 caused by the presence of these boats were verified. Behavioral registers of S. guianensis were compiled from a fixed point on the cliffs close to the study area. The observations were effected when there were no boats, in the presence of boats and after the departure of boats. The results show that type of approach by the dolphin watching boats had a major influence on the behavior of the dolphins, especially so in the groups with calves. The short term impacts were rather discrete, however, new methods should be applied in order to verify the long term impacts on the dolphins.

  17. Growth, body fatty acid composition, immune response and resistance to Streptococcus iniae of hybrid tilapia, Oreochromis niloticus X O. aureus, fed diets containing various levels of linoleic and linolenic acids

    Science.gov (United States)

    The effects of dietary linoleic (LA) and linolenic acids (LN) on growth and immunity of all-male hybrid tilapia, Oreochromis niloticus × O. aureus, were evaluated for 10 weeks. Fish fed 0.12% LA + 0% LN had the lowest weight gain (WG) but was not significantly different from diets containing 0.5% LA...

  18. Review of lobomycosis and lobomycosis-like disease (LLD) in Cetacea from South America. Scientific Committee document SC/60/DW13, International Whaling Commission, June 2008, Santiago, Chile

    OpenAIRE

    Siciliano, S.; Van Bressem, M.-F.; Moreno, I.B.; Ott, P.H.; Tavares, M.; Flores, P.A.C.; Flach, L.; Reyes, J C; M. Echegaray; Santos, M.C.O.; Viddi, F.; Crespo, E.A.; Klaich, M.J.; Félix, F; Sanino, G.P.

    2008-01-01

    Caused by a yeast-like organism known as Lacazia loboi, Lobomycosis (or lacaziosis) naturally affects humans, common bottlenose dolphins (Tursiops truncates) inhabiting coastal waters from southern Brazil to Gulf of Mexico and Atlantic coast of Florida, as well as botos-cinza (Sotalia guianensis). These species are usually found in coastal waters, subject to runoff provided by large rivers and a considerable burden of associated contaminants. Histological and morphological studies demonstrate...

  19. A STUDY ON THE GROWING OF ROMANIAN BLACK SPOTTED COWS IN SOME PRIVATE EXPLOITATIONS FROM COUNTY BOTOŞANI

    Directory of Open Access Journals (Sweden)

    RODICA DĂNĂILĂ

    2013-12-01

    Full Text Available The Romanian black spotted cows – a breed specialized in the milk production –represents the main supplier of milk in the country.The study was made to highlight the productive level and the power of hereditarytransmission of the main features thus offering data useful for the elaboration andapplication of the melioration programme for this breed in county Botoşani.

  20. ANALYSIS OF THE PUBLIC TRANSPORT PROVISION IN SUCEAVA-BOTOȘANI URBAN AREA (ROMANIA

    Directory of Open Access Journals (Sweden)

    Marian Ionuț ISTRATE

    2016-06-01

    Full Text Available Having a major economic and social role, public transport is an important component in the process of sustainable development of a city. The paper aimed to study the public transport at metropolitan and regional scale evidencing how two major urban centers of Romania, Suceava and Botoșani, connect via public transport with the rural settlements in their metropolitan areas. The metropolitan supply of public transport was analysed starting with publicly available data on the routes in 2014 for both Botoșani County and Suceava County, located in the North-Eastern side of Romania. The routes’ spatial disposition and daily frequency is analysed for road and rail public transport. The resulted mappings showed that road public transport is well distributed in the metropolitan territory for both urban centres, but there is still almost 4% of the rural population that does not have direct access to this public utility service. The rail public transport covers only a small amount of the studied area and it has a secondary role in supplying public transport services for the population because of the lack of investments that the entire rail sector suffered in Romania. The share of private cars has increased significantly, but the population’s need for mobility is not completely covered and this paper offers some recommendations for the improvement of the public transportation supply in Suceava-Botoșani Urban Area.

  1. CONCURRENT EXPERIMENTAL Streptococcus SPP. INFECTIONS AND NATURAL PARASITISM IN CHANNEL CATFISH Ictalurus punctatus

    Science.gov (United States)

    Streptococcus iniae and S. agalactiae are usually not considered pathogens of channel catfish, Ictalurus punctatus, though concurrent infections may decrease catfish survival when infected with streptococcal organisms. Non-parasitized or naturally-parasitized channel catfish fry were challenged wit...

  2. Novel method of generating non-human transgenic animals, and transgenic animals thus obtained

    OpenAIRE

    Ventura Oliveira, Pedro Nuno; Gutiérrez-Adán, Alfonso; Montoliu, Lluís

    2005-01-01

    Fecha de presentación internacional: 04.04.2005. - Titulares: Consejo Superior de Investigaciones Científicas (CSIC). - Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA)

  3. EVALUATION OF THE DNA POLYMORPHISM OF SIX VARIETIES OF CHENOPODIUM QUINOA WILLD, USING AFLP

    Directory of Open Access Journals (Sweden)

    Nolasco, Oscar

    2013-07-01

    Full Text Available The grain of Chenopodium quinoaWilld. (Quinua is important worldwide for its high nutritional value, being the principal source of proteins of the settlers of the Peruvian Bolivian altiplano. In the process of the care and maintenance of the species in the germplasm banks for quinua there have been programs developed to allow the evaluation of genetic variation to increase the quality of the grain, the resistance to diseases, and dryness tolerance and to modulate the content of saponins. A current question is to discriminate among the varieties using molecular sensitive technologies like the RAPDs, microsatellites, RFLP; our aim was to evaluate the polymorphism of six varieties of quinua using AFLP's technology (Amplified Fragment Length Polymorphism. Varieties of quinua: Quillahuaman INIA (Q, Mantaro (M, Hualhuas (H, Real Boliviana (B, Salcedo INIA(S and Illpa INIA(I, were evaluated in combinations of five pairs of primers using adapters for EcoRI and MseI to determine their polymorphisms. Our results found three combinations of major polymorphism E33/M60, E32/M48 and E32/M61, that we were able to discriminate against the variety Mantaro (M as a variety removed from the others of the southern Peruvian altiplano – Bolivian, according to UPGMAanalysis. This combination of primers also discriminate the varieties Illpa INIA and Salcedo INIA as being varieties obtained by crossings for genetic improvement.

  4. Effect of reuterin-producing Lactobacillus reuteri coupled with glycerol on the volatile fraction, odour and aroma of semi-hard ewe milk cheese.

    Science.gov (United States)

    Gómez-Torres, Natalia; Ávila, Marta; Delgado, David; Garde, Sonia

    2016-09-01

    The effect of the biopreservation system formed by Lactobacillus reuteri INIA P572, a reuterin-producing strain, and glycerol (required for reuterin production), on the volatile fraction, aroma and odour of industrial sized semi-hard ewe milk cheese (Castellano type) was investigated over a 3-month ripening period. The volatile compounds were extracted and analyzed by SPME-GC-MS and cheese odour and aroma profiles were studied by descriptive sensory analysis. Control cheese was made only with a mesophilic starter and experimental cheeses with L. reuteri were made with and without glycerol. The addition of L. reuteri INIA P572 to milk enhanced the formation of six volatile compounds. Despite the changes in the volatile compounds profile, the use of L. reuteri INIA P572 did not noticeably affect the sensory characteristics of cheese. On the other hand, the addition of L. reuteri INIA P572 coupled with 30mM glycerol enhanced the formation of twelve volatile compounds, but decreased the formation of five ones. The use of the biopreservation system did not affect overall odour and aroma quality of cheese although it resulted in a significant decrease of the odour intensity scores. In addition, this cheese received significant higher scores for "cheesy" aroma and significant lower scores for the aroma attributes "milky", "caramel" and "yogurt-like". The first two axes of a principal component analysis (PCA) performed for selected volatile compounds and sensory characteristics, accounting for 75% of the variability between cheeses, separated cheeses made with L. reuteri INIA P572 and glycerol from the rest of cheeses, and also differentiated control cheese from cheeses made with L. reuteri INIA P572 from day 60 onward. Our results showed that the reuterin-producing L. reuteri INIA P572 strain, when coupled with glycerol, may be a suitable biopreservation system to use in cheese without affecting odour and aroma quality. PMID:27289193

  5. Outgrowth inhibition of Clostridium beijerinckii spores by a bacteriocin-producing lactic culture in ovine milk cheese.

    Science.gov (United States)

    Garde, Sonia; Avila, Marta; Arias, Ramón; Gaya, Pilar; Nuñez, Manuel

    2011-10-17

    In the manufacture of model cheeses, ovine milk was deliberately contaminated with spores of Clostridium beijerinckii INIA 63, a wild isolate from Manchego cheese with late blowing defect, and inoculated with nisin- and lacticin 481-producing Lactococcus lactis subsp. lactis INIA 415 as starter, to test its potential to prevent the late blowing defect, or with L. lactis subsp. lactis INIA 415-2, a spontaneous mutant not producing bacteriocins. Cheeses made individually with the lactococcal strains, without clostridial spores, served as controls. Cheese made with clostridial spores and L. lactis subsp. lactis INIA 415-2 showed late blowing defect after 120days of ripening. Spoilt cheese also showed lower concentrations of lactic acid, and higher levels of acetic, propionic and butyric acids, and of other volatile compounds such as 2-propanol and 1-butanol, than control cheese. In addition, cheese made with the bacteriocin producer did not show any late blowing symptoms, despite its spore counts similar to those of blown cheese, pointing to outgrowth inhibition of C. beijerinckii spores by bacteriocins. Besides, cheese made with the bacteriocin producer showed similar concentrations of lactic acid and volatile compounds than control cheese. Inclusion of L. lactis subsp. lactis INIA 415 in starter cultures seems a feasible method to prevent late blowing defect in cheese without altering its sensory characteristics. PMID:21849216

  6. Aspectos histológicos do trato digestivo de Sotalia fluviatilis (Cetacea, Delphinidae: esôfago e estômago

    Directory of Open Access Journals (Sweden)

    Paulo de Tarso da C. Chaves

    1988-07-01

    Full Text Available Os esôfagos e estômagos de seis botos tucuxi, Sotalia fluviatilis (Cetacea: Delphinidae foram examinados anatômica e microscopicamente e comparados com os de botos marinhos do mesmo gênero. O esôfago é um simples tubo distensível e macroscopicamente uniforme até sua junção com o estômago. Este boto tem um estômago tricompartimentado: uma parte anterior ou muscular; uma principal, glandular; e uma pilórica. As características anatomo-funcionais de cada compartimento são descritas. Não se achou esfíncter entre o esôfago e o estômago. Em comparação com os delfinídeos marinhos, não há adaptações, por parte de Sotalia, à invasão relativamente recente do ambiente de água doce.The oesophagus and stomachs of six tucuxi dolphins, Sotalia fluviatilis (Cetacea: Delphinidae were examined anatomically and microscopically and compared with marine dolphins of the same genus. The oesophagus is a simple distendable tube and is macroscopically uniform to its junctureh with te stomach. This dolphin has a three-compartmented stomach: an anterior or muscular compartment; a principal one, which is glandular; and the pyloric part. The anatomical and functional characteristics of each compartment is described. No sphincter was found between the oesophagus and the stomach. In comparison with marine delphinids there are no adaptations specific to the relatively recent invasion of the fresh-water environment by Sotalia.

  7. Relações intersemióticas entre literatura, cinema, artes plásticas e desenho em dois romances de Márcio Souza

    Directory of Open Access Journals (Sweden)

    Luís Heleno Montoril del Castilo

    2013-01-01

    Full Text Available This paper aims to explore the relationships between different artistic forms – li- terature, cinema, plastic arts and design – investigating the operation of the bond- ing techniques (originating end of the plastic arts and assembly (film technique and how they were implemented in literature. To this end, we as an object of analysis the novels of the writer amazon Márcio Souza Galvez imperador do Acre (1983 and A resistível ascensão do Boto Tucuxi ([1977] 1982.

  8. Python and AWS Cookbook

    CERN Document Server

    Garnaat, Mitch

    2011-01-01

    If you intend to use Amazon Web Services (AWS) for remote computing and storage, Python is an ideal programming language for developing applications and controlling your cloud-based infrastructure. This cookbook gets you started with more than two dozen recipes for using Python with AWS, based on the author's boto library. You'll find detailed recipes for working with the S3 storage service as well as EC2, the service that lets you design and build cloud applications. Each recipe includes a code solution you can use immediately, along with a discussion of why and how the recipe works. You al

  9. Quality consumption of strawberries (Fragaria ananassa

    Directory of Open Access Journals (Sweden)

    Sergio Carballo

    2011-04-01

    Full Text Available Consumers increasingly require a food product that preserves their nutritional value; retain fresh colour, flavour and texture.Appearance, texture and flavour are attributes identified as to be key to acceptability of fruit and also for consumers in making purchase of fresh produce.The objective of this work was to investigate the consumer's preference of sensory quality and the commercial aspects of fresh strawberries, harvested in spring time from the south of our country, Uruguay- South America.In this study, seven different strawberries varieties (INIA-Arazá, INIA- Ivapitá, Ventana, Seascape, Camarosa, INIA- Ivahé, Aromas were sensory and physicochemical evaluated.The results obtained showed a high correlation between total soluble solids and sensory attributes (colour, texture, and flavour.Camarosa and Ivahé varieties were prefered for the external and internal aspects of the fruit.Future work should investigate other producer areas of the country and other harvesting time.

  10. New $B^{\\pm}\\to K\\pi$ data explain absence of CP violation Tree-penguin interference canceled by Pauli effects

    CERN Document Server

    Lipkin, Harry J

    2011-01-01

    Observation of CP violation in $B^o\\to K^\\pm\\pi^{\\mp}$ decays and its absence in $B^+\\to K^+\\pi^o$ decays are explained in new improved data analysis of more precise $B\\to K\\pi$ data. Success of the "Lipkin Sum Rule" indicates that four $B\\tow K\\pi$ branching ratios are determined by three parameters, the penguin diagram $P$ and two interference terms $P\\cdot T$ and $P\\cdot S$ between the dominant penguin and two tree diagrams; the color-favored and color suppressed diagrams. Previous analyzes confirmed the model with errors leaving values of interference terms less that two standard deviations from zero. The observation CP violation in $B^o\\to K^\\pm\\pi^{\\mp}$ decays indicates a finite value for $P\\cdot T$. New precise data analysis show $P\\cdot T$ and $P\\cdot S$ interference contributions well above errors. Their contributions to $B^\\pm\\to K\\pi$ decays are shown to be nearly equal with opposite phase and cancel within experimental errors. This cancelation unexpected in previous analyzes explains the failure ...

  11. El agua en la agricultura: gestión y uso sostenibles

    OpenAIRE

    Aragüés Lafarga, Ramón

    2009-01-01

    1 fichero .pdf (31 Pags.) copia de la presentación original del autor en el Seminario. Esta Presentación es Síntesis de la conferencia impartida en el VII encuentro del foro de los INIA de Iberoamérica el 30 de junio de 2008 en Huesca.

  12. STREPTOCOCCUS: A WORLDWIDE FISH HEALTH PROBLEM

    Science.gov (United States)

    Streptococcus iniae and S. agalactiae are important emergent pathogens that affect many fish species worldwide, especially in warm-water regions. In marine and freshwater systems, these Gram-positive bacteria cause significant economic losses, estimated at hundreds of millions of dollars annually. ...

  13. 硝唑尼特--一种新的抗原虫药

    Institute of Scientific and Technical Information of China (English)

    陈阜新; 韩洪刚

    2004-01-01

    @@ 硝唑尼特(nitazoxanid,商品名A1inia)是一种新的抗原虫药,由美国FDA批准其液体制剂治疗隐孢子虫病(Crytosporidium parvum)和肠贾第虫(Giardia lamblia)所致1~11岁儿童的腹泻.

  14. Inhibition of Listeria monocytogenes by enterocin 4 during the manufacture and ripening of Manchego cheese.

    Science.gov (United States)

    Nuñez, M; Rodríguez, J L; García, E; Gaya, P; Medina, M

    1997-12-01

    The inhibitory effect of enterocin 4, a bacteriocin produced by Enterococcus faecalis INIA 4, on Listeria monocytogenes strains Ohio and Scott A during the manufacture and ripening of Manchego cheese was investigated. Raw ewe's milk was inoculated with ca 10(5) cfu ml-1 of L. monocytogenes and with 1% of a commercial lactic starter, 1% of an Ent. faecalis INIA 4 culture, or 1% of each culture. Manchego cheeses were manufactured according to usual procedures. Listeria monocytogenes Ohio counts decreased by 3 log units after 8 h and by 6 log units after 7 d in cheese made from milk inoculated with Ent. faecalis INIA 4 or with both cultures, whereas no inhibition was recorded after 60 d in cheese made from milk inoculated with commercial lactic starter. Listeria monocytogenes Scott A was not inhibited by enterocin 4 during cheese manufacture, but decreases of 1 log unit after 7 d and of 2 log units after 60 d were achieved in cheese made from milk inoculated with both commercial lactic starter and Ent. faecalis INIA 4. PMID:9449804

  15. Fish Vaccine Development and Use to Prevent Streptococcal Diseases

    Science.gov (United States)

    An important pathogen of tilapia, hybrid striped bass and trout raised in intensive aquaculture is Streptococcus sp., a cause of severe economic losses in the fish farming industry. Infected fish experience severe to moderate mortality due to Streptococcus iniae and/or S. agalactiae. The diseased ...

  16. Molecular epidemiology of zoonotic streptococcosis/lactococcosis in rainbow trout (Oncorhynchus mykiss aquaculture in Iran

    Directory of Open Access Journals (Sweden)

    G Nikbakhat-Brojeni

    2010-12-01

    Full Text Available Background and Objectives: Streptococcosis/lactococcosis is a hyperacute systemic disease that can occur in marine and fresh waters of many species of fish. The aim of this work was to study the disease outbreak in the major rainbow trout (Oncorhynchus mykiss production of Iran."nMaterials and Methods: 108 Gram positive cocci isolates were obtained from diseased trout in seven provinces with major trout production during 2008 till 2009. These bacterial isolates were characterized using phenotypic and molecular studies. The isolates were also analysed phylogeneticaly and compared with the available data."nResults: 49 samples (45.37% were identified as Streptococcus iniae, 37 samples (35.2% matched with Lactococcus garvieae; and 22 samples (19.43% were identified as members of Streptooccus genus by culture-based and biochemical tests of API 50 CH, API 20 STREP and rapid 32 STREP systems. Using universal primers for differentiation of Streptococcus sp. and Enterococcus sp, all 108 samples were identified as Streptococcus sp. with a target region of 500 bp. Single specific PCR resulted in identification of 64 (59.2% isolates as S. iniae and 44 (40.8% isolates as L. garvieae. The phylogenetic analysis of the S. iniae isolates resulted in maximal similarity to some strains reported from Taiwan and to all Brazilian strains. Also, one strain showed less sequence similarity values with other tested strains although this strain has high similarity with ATCC 29178 strain, all reported Chinese, and some Taiwanian strains. Also, analysis of S. iniae LctO gene sequence showed that this isolate clustered within the S. iniae group. The sequence analysis of L. garvieae strains also showed that they have maximum similarity to all Japanese and Chinese strains, but one strain has lower sequence similarity values with all other recorded strains."nConculsion: The results of this study clearly show that trout farming in Iran is severely affected by both species of S

  17. Papildai, kuriais prekiaujama Lietuvoje - atitiktis teisės aktų nuostatoms, vartotojų nuomonės analizė

    OpenAIRE

    Prazarkevičiūtė, Virginija

    2014-01-01

    Darbo tikslas – įvertinti Lietuvos rinkoje parduodamų maisto papildų ženklinimo atitiktį teisės aktams bei vartotojų nuomonę apie maisto papildus. Rengiant darbą buvo iškelti uždaviniai: 1. Apibendrinti NMVRVI atliekamus maisto papildų tyrimus. 2. Išanalizuoti vartotojų žinias apie maisto papildus. Per darbo rašymo laikotarpį buvo vykdoma anoniminė, internetinė apklausa, kurios pagalba buvo norėta išsiaiškinti vartotojų žinias apie maisto papildus, bei kokią įtaką jų pasirinkimui turi sveikat...

  18. Isolation and characterization of bacteria with antibacterial properties from Nile tilapia (Oreochromis niloticus).

    Science.gov (United States)

    Etyemez, Miray; Balcazar, Jose Luis

    2016-04-01

    One hundred and twenty bacterial isolates were obtained from the intestinal mucus of Nile tilapia (Oreochromis niloticus) and screened for antagonistic activity and adherence abilities. Based on in vitro antagonism against two pathogens (Streptococcus iniae and Edwardsiella piscicida), five isolates were selected and identified by 16S rRNA gene sequence analysis. All antagonistic isolates were affiliated to the genus Bacillus, which showed inhibitory activity against S. iniae. Only the isolate B191 (closely related to Bacillus mojavensis) inhibited the growth of both pathogens. Moreover, isolate B191 adhered significantly better to fish intestinal mucus than other antagonistic isolates. According to our results, these bacterial isolates, particularly isolate B191, should be further studied to explore their probiotic effects under in vivo conditions. PMID:27033910

  19. Molecular epidemiology of zoonotic streptococcosis/lactococcosis in rainbow trout (Oncorhynchus mykiss) aquaculture in Iran

    DEFF Research Database (Denmark)

    Karsidani, S. Haghighi; Soltani, M.; Nikbakhat-Brojeni, G.;

    2010-01-01

    ."nMaterials and Methods: 108 Gram positive cocci isolates were obtained from diseased trout in seven provinces with major trout production during 2008 till 2009. These bacterial isolates were characterized using phenotypic and molecular studies. The isolates were also analysed phylogeneticaly and compared...... similarity values with all other recorded strains."nConculsion: The results of this study clearly show that trout farming in Iran is severely affected by both species of S. iniae and L. garvieae and requires serious preventive criteria....

  20. Characterization and identification of streptococci from golden pompano in China.

    Science.gov (United States)

    Cai, X H; Peng, Y H; Wang, Z C; Huang, T; Xiong, X Y; Huang, Y C; Wang, B; Xu, L W; Wu, Z H

    2016-05-26

    Streptococcal infections cause significant mortality and high economic losses in the fish farm industry worldwide, including in the culture of golden pompano Trachinotus ovatus L., a species gaining popularity in China. A total of 9 streptococcal strains were isolated from cage-cultured diseased golden pompano in Beihai, Zhanjing, and Shenzhen, China, between 2012 and 2014. Conventional and rapid identification systems were used to determine that the isolates were Streptococcus agalactiae, S. iniae, and S. dysgalactiae subsp. dysgalactiae. All isolates were gram-positive cocci cells in pairs or short-chain, non-motile, catalase negative, α or β hemolytic cocci. The results of multiplex PCR assays and 16S rRNA BLAST analysis also showed that the β hemolytic strains were S. agalactiae and S. iniae and the α hemolytic strain was S. dysgalactiae subsp. dysgalactiae, respectively. Pathogenicity assays revealed that S. agalactiae (lethal dose [LD50]: 6.38 × 10(4) CFU ml(-1)) was more virulent for golden pompano than S. iniae (LD50: 1.47 × 10(7) CFU ml(-1)) and S. dysgalactiae subsp. dysgalactiae (LD50: 2.57 × 10(6) CFU ml(-1)) when they were challenged by intraperiotoneal (i.p.) injection. The results of antibiotic susceptibility showed that all strains were extremely susceptible to cefradine, erythromycin, and cefotaxime but resistant to gentamicin, penicillin G, novobiocin, neomycin, ciprofloxacin, roxithromycin, furazolidone, enrofloxacin, norfloxacin, kanamycin, ampicillin, tetracycline, and vancomycin This is the first report of a phenomenon of golden pompano coinfection with S. agalactiae and S. iniae, which will contribute to the diagnosis and prevention of streptococcicosis. PMID:27225204

  1. Cold tolerance evaluation in Chilean rice genotypes at the germination stage

    Directory of Open Access Journals (Sweden)

    Gabriel Donoso Ñanculao

    2013-03-01

    Full Text Available Low temperature is the most important abiotic stress affecting rice (Oryza sativa L. yield in Chile. Rice in Chile is usually planted when the minimum air temperatures are below 12 °C. This temperature is lower than the optimum needed for normal rice germination. Therefore, the aim of this study was to evaluate cold tolerance in 20 experimental lines from the Rice Breeding Program of the Instituto de Investigaciones Agropecuarias (INIA, Chile, at the germination stage. Coleoptile length reduction (CRED, coleoptile length after cold treatment (CLEN, coleoptile length recovery (CREC, and coleoptile regrowth (CREG were evaluated at 13 °C for 4 d using 'Diamante-INIA' as the cold-tolerant control. To find genotypes with cold tolerance (low CRED value and high CLEN, CREC, and CREG values, genotypes were ranked, a biplot of principal components, and cluster analysis were performed. No differences were found among genotypes in the ranking based on CREC value so this trait was not considered. Analysis showed that only three experimental lines had cold tolerance similar to that of 'Diamante-INIA'; all other experimental lines exhibited intermediate to low cold tolerance. These results showed low cold tolerance of some Chilean genotypes at the germination stage, thus confirming the need to evaluate the rest of the germplasm from the Rice Breeding Program.

  2. Guiana dolphins (Sotalia guianensis displaying beach hunting behavior in the Cananéia Estuary, Brazil: social context and conservation issues

    Directory of Open Access Journals (Sweden)

    Marcos César de Oliveira Santos

    2010-06-01

    procura e captura de alimento em praias (“beach hunting” exibido por botos-cinza (Sotalia guianensis no Estuário de Cananéia (EC (25ºS, 48ºW, um estudo baseado na aplicação da técnica de foto-identificação foi conduzido de maio de 2000 a julho de 2003. Três plataformas de observação a foram escolhidas na principal entrada do mencionado estuário, onde os pesquisadores encontram uma oportunidade ímpar de observar os botos a uma pequena distância sem incomodá-los. As observações foram oportunísticas e desigualmente distribuídas ao longo do tempo e do espaço. Adicionadas às observações de identificação individual, uma balestra de 80lbs de pressão foi utilizada para coletar amostras de pele de oito indivíduos monitorados com vistas à determinação do sexo. Em 67 dias de investigação, que renderam aproximadamente 80h de observações diretas dos botos, 4.102 fotografias foram tomadas, das quais 1.098 (26,8% foram consideradas úteis para o propósito de identificação individual. Um total de 103 grupos foi observado (3,7 ± 2,6 indivíduos, variando entre botos solitários e 15 indivíduos. Pares de fêmeas e filhotes foram observados em 92,4% dos grupos investigados. Apresenta-se a primeira notificação de intervalo de nascimento de um filhote para o EC: aproximadamente 3 anos e 9 meses. De 40 indivíduos identificados ao menos uma vez, onze usaram regularmente as praias: fêmeas KN #s 10, 30, 255, 268 e 279; machos KN #s 86 e 257; e quatro indivíduos de sexo desconhecido. As características das associações entre os indivíduos monitorados foram avaliadas através das análises de índices de associação. Um total de 83 associações possíveis entre pares de botos foi analisado utilizando o índice de peso médio e dois critérios de seleção (5+ e 8+ avistamentos/indivíduo. A média dos índices de associação variou entre 0,12 e 0,16 evidenciando laços fracos entre os indivíduos monitorados, cuja maioria foi composta por f

  3. The behavior of Sotalia guianensis (Van Bénéden in Pernambuco coastal waters, Brazil, and a further analysis of its reaction to boat traffic O comportamento de Sotalia guianensis (Van Bénéden em águas costeiras de Pernambuco, Brasil, e uma análise adicional de sua reação ao tráfego de embarcações

    Directory of Open Access Journals (Sweden)

    Janaina P. Araújo

    2008-03-01

    Full Text Available There is still little knowledge about the behavior of the estuarine dolphins and also the way they react to the presence of different types of vessels. The aim of the present study was twofold: to investigate the behavior of estuarine dolphins in four locations in Pernambuco, Brazil, and also their reaction to the presence of common types of nautical crafts. The data of this study resulted from 48 fieldtrips to each of the four localities studied (ports of Recife and Suape, and beaches of Bairro Novo and Piedade, thus totaling 192 days of observations, with approximately 380 hours of actual sampling. Ten types of behavioral activities were counted for Bairro Novo beach and the port areas, but only four for Piedade beach. The greatest flows of boats were recorded in the port of Recife. Fishing boats were the commonest type in the port area of Recife and Bairro Novo beach, while tourist vessels predominated in the port of Suape and Piedade beach. During the observations of encounters between these boats and the estuarine dolphins, neutral reactions predominated for all the vessel types studied. The same type of reaction predominated when the distances at which the interactions between the animals and vessels occurred were correlated.Ainda se sabe pouco sobre o comportamento do boto-cinza, assim como a forma pela qual ele reage a diferentes tipos de embarcações. O presente estudo teve dois objetivos: investigar o comportamento dos botos-cinzas em quatro localidades de Pernambuco, Brasil, assim como a reação destes para com as embarcações náuticas comuns. Os dados foram coletados no período de setembro de 2004 a agosto de 2006, no horário das 6 às 17 h. Os dados desta pesquisa resultaram de 48 saídas a campo para cada uma das quatro localidades estudadas (Portos do Recife e Suape, praia de Bairro Novo e Piedade, totalizando 192 dias de observações, sendo aproximadamente 380 horas de amostragens efetivas. Foram contados dez tipos de

  4. Evaluation of reference genes for quantitative real-time RT-PCR analysis of gene expression in Nile tilapia (Oreochromis niloticus).

    Science.gov (United States)

    Yang, Chang Geng; Wang, Xian Li; Tian, Juan; Liu, Wei; Wu, Fan; Jiang, Ming; Wen, Hua

    2013-09-15

    Quantitative real-time reverse-transcriptase polymerase chain reaction (RT-qPCR) has been used frequently to study gene expression related to fish immunology. In such studies, a stable reference gene should be selected to correct the expression of the target gene. In this study, seven candidate reference genes (glyceraldehyde-3-phosphate dehydrogenase (GADPH), ubiquitin-conjugating enzyme (UBCE), 18S ribosomal RNA (18S rRNA), beta-2-microglobulin (B2M), elongation factor 1 alpha (EF1A), tubulin alpha chain-like (TUBA) and beta actin (ACTB)), were selected to analyze their stability and normalization in seven tissues (liver, spleen, kidney, brain, heart, muscle and intestine) of Nile tilapia (Oreochromis niloticus) challenged with Streptococcus agalactiae or Streptococcus iniae, respectively. The results showed that all the candidate reference genes exhibited tissue-dependent transcriptional variations. With PBS injection as a control, UBCE was the most stable and suitable single reference gene in the intestine, liver, brain, kidney, and spleen after S. iniae infection, and in the liver, kidney, and spleen after S. agalactiae infection. EF1A was the most suitable in heart and muscle after S. iniae or S. agalactiae infection. GADPH was the most suitable gene in intestine and brain after S. agalactiae infection. In normal conditions, UBCE and 18S rRNA were the most stably expressed genes across the various tissues. These results showed that for RT-qPCR analysis of tilapia, selecting two or more reference genes may be more suitable for cross-tissue analysis of gene expression.

  5. Antibacterial Activities of Metabolites from Platanus occidentalis (American sycamore) against Fish Pathogenic Bacteria

    Science.gov (United States)

    Schrader, Kevin K; Hamann, Mark T; McChesney, James D; Rodenburg, Douglas L; Ibrahim, Mohamed A

    2016-01-01

    One approach to the management of common fish diseases in aquaculture is the use of antibiotic-laden feed. However, there are public concerns about the use of antibiotics in agriculture and the potential development of antibiotic resistant bacteria. Therefore, the discovery of other environmentally safe natural compounds as alternatives to antibiotics would benefit the aquaculture industries. Four natural compounds, commonly called platanosides, [kaempferol 3-O-α-L-(2″,3″-di-E-p-coumaroyl)rhamnoside (1), kaempferol 3-O-α-L-(2″-E-p-coumaroyl-3″-Z-p-coumaroyl)rhamnoside (2), kaempferol 3-O-α-L-(2″-Z-p-coumaroyl-3″-E-p-coumaroyl)rhamnoside (3), and kaempferol 3-O-α-L-(2″,3″-di-Z-p-coumaroyl)rhamnoside (4)] isolated from the leaves of the American sycamore (Platanus occidentalis) tree were evaluated using a rapid bioassay for their antibacterial activities against common fish pathogenic bacteria including Flavobacterium columnare, Edwardsiella ictaluri, Aeromonas hydrophila, and Streptococcus iniae. The four isomers and a mixture of all four isomers were strongly antibacterial against isolates of F. columnare and S. iniae. Against F. columnare ALM-00-173, 3 and 4 showed the strongest antibacterial activities, with 24-h 50% inhibition concentration (IC50) values of 2.13 ± 0.11 and 2.62 ± 0.23 mg/L, respectively. Against S. iniae LA94-426, 4 had the strongest antibacterial activity, with 24-h IC50 of 1.87 ± 0.23 mg/L. Neither a mixture of the isomers nor any of the individual isomers were antibacterial against isolates of E. ictaluri and A. hydrophila at the test concentrations used in the study. Several of the isomers appear promising for the potential management of columnaris disease and streptococcosis in fish.

  6. Evidence of vertical transmission and tissue tropism of Streptococcosis from naturally infected red tilapia (Oreochromis spp.

    Directory of Open Access Journals (Sweden)

    Padmaja Jayaprasad Pradeep

    2016-05-01

    Full Text Available Streptococcosis is a highly problematic disease in the aquaculture of freshwater fishes, especially for tilapia. The possibility of vertical transmission of streptococcosis and the pattern of tissue tropism of this pathogen in various organs was examined in red tilapia (Oreochromis sp.. Healthy broodstock without any clinical signs of Streptococcus spp. were selected from a farm earlier reported to have the disease and a total of 10 pairs were forced spawned to provide samples of gametes and progeny for pathogen testing. A colorimetric LAMP assay was used to confirm whether the bacterial pathogens Streptococcus. agalactiae and Streptococcus. iniae was present in samples of milt, unfertilized eggs, fertilized eggs, and offspring at various stages of development, as well as internal organs of broodstock (reproductive organs, gill, liver, spleen, kidney and brain as well as samples of water from culture systems. The majority of samples of milt (9/10 and unfertilized eggs (7/10 collected from the broodstock were infected with S. iniae at the time of spawning and was transmitted to all of their offspring. Nevertheless, when the same samples of gametes were analyzed for S. agalactiae, they were all found to be negative but the pathogen was found to be present in some 10-day-old larval offspring (4/10. However, when the pathogenic presence was analyzed from the reproductive organs of the parents, both S. agalactiae (11/20 and S. iniae (18/20 bacterium were common. Although, all broodstock were asymptomatic, almost all broodstock harboured the bacteria in many organs. Confirmation of vertical transmission of streptococcosis in tilapia means that intergenerational break cannot be used as a reliable and simple means of reducing or eliminating the prevalence of these difficult pathogens in aquaculture stock.

  7. Isthminia panamensis, a new fossil inioid (Mammalia, Cetacea) from the Chagres Formation of Panama and the evolution of ‘river dolphins’ in the Americas

    Science.gov (United States)

    Vélez-Juarbe, Jorge; Gutstein, Carolina S.; Little, Holly; Vigil, Dioselina; O’Dea, Aaron

    2015-01-01

    In contrast to dominant mode of ecological transition in the evolution of marine mammals, different lineages of toothed whales (Odontoceti) have repeatedly invaded freshwater ecosystems during the Cenozoic era. The so-called ‘river dolphins’ are now recognized as independent lineages that converged on similar morphological specializations (e.g., longirostry). In South America, the two endemic ‘river dolphin’ lineages form a clade (Inioidea), with closely related fossil inioids from marine rock units in the South Pacific and North Atlantic oceans. Here we describe a new genus and species of fossil inioid, Isthminia panamensis, gen. et sp. nov. from the late Miocene of Panama. The type and only known specimen consists of a partial skull, mandibles, isolated teeth, a right scapula, and carpal elements recovered from the Piña Facies of the Chagres Formation, along the Caribbean coast of Panama. Sedimentological and associated fauna from the Piña Facies point to fully marine conditions with high planktonic productivity about 6.1–5.8 million years ago (Messinian), pre-dating the final closure of the Isthmus of Panama. Along with ecomorphological data, we propose that Isthminia was primarily a marine inhabitant, similar to modern oceanic delphinoids. Phylogenetic analysis of fossil and living inioids, including new codings for Ischyrorhynchus, an enigmatic taxon from the late Miocene of Argentina, places Isthminia as the sister taxon to Inia, in a broader clade that includes Ischyrorhynchus and Meherrinia, a North American fossil inioid. This phylogenetic hypothesis complicates the possible scenarios for the freshwater invasion of the Amazon River system by stem relatives of Inia, but it remains consistent with a broader marine ancestry for Inioidea. Based on the fossil record of this group, along with Isthminia, we propose that a marine ancestor of Inia invaded Amazonia during late Miocene eustatic sea-level highs. PMID:26355720

  8. Isthminia panamensis, a new fossil inioid (Mammalia, Cetacea) from the Chagres Formation of Panama and the evolution of 'river dolphins' in the Americas.

    Science.gov (United States)

    Pyenson, Nicholas D; Vélez-Juarbe, Jorge; Gutstein, Carolina S; Little, Holly; Vigil, Dioselina; O'Dea, Aaron

    2015-01-01

    In contrast to dominant mode of ecological transition in the evolution of marine mammals, different lineages of toothed whales (Odontoceti) have repeatedly invaded freshwater ecosystems during the Cenozoic era. The so-called 'river dolphins' are now recognized as independent lineages that converged on similar morphological specializations (e.g., longirostry). In South America, the two endemic 'river dolphin' lineages form a clade (Inioidea), with closely related fossil inioids from marine rock units in the South Pacific and North Atlantic oceans. Here we describe a new genus and species of fossil inioid, Isthminia panamensis, gen. et sp. nov. from the late Miocene of Panama. The type and only known specimen consists of a partial skull, mandibles, isolated teeth, a right scapula, and carpal elements recovered from the Piña Facies of the Chagres Formation, along the Caribbean coast of Panama. Sedimentological and associated fauna from the Piña Facies point to fully marine conditions with high planktonic productivity about 6.1-5.8 million years ago (Messinian), pre-dating the final closure of the Isthmus of Panama. Along with ecomorphological data, we propose that Isthminia was primarily a marine inhabitant, similar to modern oceanic delphinoids. Phylogenetic analysis of fossil and living inioids, including new codings for Ischyrorhynchus, an enigmatic taxon from the late Miocene of Argentina, places Isthminia as the sister taxon to Inia, in a broader clade that includes Ischyrorhynchus and Meherrinia, a North American fossil inioid. This phylogenetic hypothesis complicates the possible scenarios for the freshwater invasion of the Amazon River system by stem relatives of Inia, but it remains consistent with a broader marine ancestry for Inioidea. Based on the fossil record of this group, along with Isthminia, we propose that a marine ancestor of Inia invaded Amazonia during late Miocene eustatic sea-level highs.

  9. Identificación de Cultivares y Líneas de Mejoramiento de Arroz de Chile Mediante Amplificación de Fragmentos Polimórficos (AFLP) Identification of Chilean Rice Cultivars And Breeding Lines by Means of Amplified Fragment Length Polymorphism (AFLP)

    OpenAIRE

    Carlos Aguirre; Roberto Alvarado; Patricio Hinrichsen

    2005-01-01

    Doce cultivares y líneas de arroz (Oryza sativa L.) desarrollados por el programa de fitomejoramiento del Instituto de Investigaciones Agropecuarias (INIA), Centro Regional de Investigación Quilamapu, fueron caracterizados genéticamente mediante amplificación de fragmentos polimórficos (AFLP). De 21 combinaciones ensayadas, sólo 16 fueron informativas, generando un total de 667 amplicones, 94 de ellos polimórficos (14,4%), con un rango entre 9 y 33% de polimorfismo por combinación. Esto indic...

  10. Causas de mortalidad de alpacas en tres principales centros de producción ubicados en puna seca y humeda del departamento de Puno (Causes of mortality of alpacas in three main centers of production located in dry and humid fist of the Puno department)

    OpenAIRE

    Mamani Paredes, Javier; Condemayta Condemayta, Zacarias; Calle Charaja, Leoncio

    2009-01-01

    ResumenEl presente estudio se efectuó en tres principales centros de producción alpaquera del departamento de Puno: Rural Alianza EPS, CIP “La Raya” – UNA Puno ubicados en la zona agro ecológica de Puna húmeda y Anexo Quimsachata de INIA – Puno ubicado en la zona agro ecológica de Puna seca; con el objetivo de determinar las principales causas de mortalidad de alpacas. Mediante un estudio observacional analítico de tipo retrospectivo, utilizando los registros de mortalidad de las campañas de ...

  11. Noves tècniques serològiques per detectar anticossos enfront el circovirus porcí

    OpenAIRE

    Pérez Martín, Eva

    2009-01-01

    Des del Centre de Recerca en Sanitat Animal (CRESA), amb la col·laboració del Departament de Biotecnologia de l’INIA, s’han desenvolupat dos nous tests serològics (ELISAs) per detectar anticossos del circovirus, l’agent responsable de moltes de les malalties que pateix l’espècie porcina. Aquestes dues noves tècniques es basen en proteïnes recombinants expressades en larves de l’insecte Trichoplusia ni. Per incrementar el rendiment d’aquestes proteïnes, les larves han estat inoculades amb bacu...

  12. EcoLogic Reader

    OpenAIRE

    Šeikienė, Nijolė

    2008-01-01

    Mokymo priemonė skirta aplinkos apsaugos studijų programos studentams. Ją sudaro abėcėliniai tekstų rinkinėliai, atrinkta ir paryškinta specialybinė leksika bei įvairūs įtvirtinamieji pratimai. Knygos pabaigoje spausdinami testai ir tekstai geresniam žodyno suvokimui bei turtinimui. Medžiaga atspindi šiandienines aktualijas, ji gali būti naudinga kiekvienam, norinčiam pagilinti anglų kalbos žinias ekologine tematika. The EcoLogic Reader is an educational book for students of ecology. It pr...

  13. Altruizmas ir jaunimo altruistiškumo ugdymo veiksniai

    OpenAIRE

    Lileikis, Saulius

    2004-01-01

    Straipsnyje atskleisti altruizmo koncepcijos ištakų bei raidos momentai ir, remiantis dorovinės pozicijos koncepcija, kuri implikuoja žinias apie dorovines vertybes, dorovinius požiūrius ir su jais susijusius išgyvenimus, siekius realizuoti dorovines vertybes ir konkrečių veiksmų sąveiką, ištirta jaunimo pozicija jų altruistiškumo ugdymo veiksnių atžvilgiu. The present article analyzes the beginning and development of conception of altruism. on the ground of conception of the moral positio...

  14. CLONACIÓN Y FILOGENIA MOLECULAR DE UN SEGMENTO DEL GEN CODANTE DE LA ACTINA DE MYRCIARIA DUBIA “CAMU-CAMU”: UN CANDIDATO PARA GEN DE REFERENCIA

    OpenAIRE

    Juan Castro Gómez; Marianela Cobos Ruiz; Alina Egoavil Reátegui; Roberson Ramírez Saavedra; Sixto Imán Correa; Pedro Adrianzen Julca; Jorge Marapara del Águila

    2012-01-01

    Myrciaria dubia “camu-camu” es un frutal amazónico caracterizado por su amplia variación de vitamina C. Pero los estudios genético moleculares que puedan explicar esta variación son limitados. Por ello nuestro objetivo fue realizar la clonación y filogenia molecular de un segmento del gen codante de la actina de M. dubia. Las muestras fueron obtenidas de la colección de germoplasma del INIA. Luego, el ARN fue purificado y mediante RT-PCR con cebadores degenerados se amplificó un segmento del ...

  15. Potassium and Phosphorus in Muscat Rosada Grape Yield in Elqui Valley Soil Fósforo y Potasio en la Producción de Vid Moscatel Rosada, en Suelo del Valle de Elqui

    OpenAIRE

    Carlos Sierra B; Rubén Alfaro P

    2008-01-01

    The effects of P and K on grapevines (Vitis vinifera L.) var. Muscat Rosada were evaluated four years, considering the same levels of N. The experiment was conducted at the Vicuña Experimental Station (30° S; 70°44´ W) of the Instituto de Investigaciones Agropecuarias (INIA). The soil is alluvial antropic miscellaneous (Entisols). Three fertilization treatments were established: 1) N 160 + P2O5 200 + K2O 300; 2) N 160 + P2O5 0 + K2O 300; and 3) N 160 + P2O5 200 + K2O 0. At the beginning of th...

  16. MEJORAMIENTO DEL PORCENTAJE DE PROTEÍNA EN MAIZ PARA ENSILAJE CON EL AUMENTO Y PARCIALIZACIÓN DE LA FERTILIZACIÓN NITROGENADA Improvement of protein percentage in corn silage with an increase in and partitioning of nitrogen fertilization

    OpenAIRE

    Patricio Soto O.; Ernesto Jahn B; Susana Arredondo S

    2004-01-01

    En suelos arcillosos de mal drenaje se realizaron dos ensayos con el objetivo de determinar la respuesta de maíz (Zea mays L.) para ensilaje a la aplicación de N en el rendimiento y contenido de proteína del forraje. En el Ensayo 1 los tratamientos correspondieron a dos híbridos de maíz para ensilaje de distinta precocidad, SX-43 e INIA-150, sembrados con una población de 90.000 plantas ha-1 y cuatro dosis de N: 0, 100, 200 y 400 kg N ha-1. En el Ensayo 2, los tratamientos fueron dos dosis de...

  17. Los inicios de la modernización de la investigación agraria

    Directory of Open Access Journals (Sweden)

    Navarro, Luis

    2007-10-01

    Full Text Available The Spanish Government and the International Bank for Reconstruction and Development (BIRF signed a cooperation agreement in 1971 with the objective to upgrade the level of agricultural research by: a restructuring the National Crop Research Institute (INIA to create commodity oriented National Centers, b changing research planning through the creation of National Commodity oriented Programs, and c training of 200 scientists in international high quality laboratories in the priority areas of Research Centers. Total budget of the project was 28,2 million dollars (equivalent today to about 204 million euros, with BIRF supporting 12,7 million dollars as credit. The project successfully accomplished its main objectives and caused an important change in the quality and orientation of agricultural research that had an high impact from scientific and socio economic points of view. This was specially due to the training program, since the other achievements were loosed after the transfer of INIA regional Centers to the Autonomous Communities.El Gobierno de España y el Banco Internacional de Reconstrucción y Fomento (BIRF firmaron en 1971 un Proyecto de colaboración con el objetivo de mejorar el nivel de la investigación agraria mediante tres estrategias fundamentales: a reestructuración del Instituto Nacional de Investigaciones Agrarias (INIA para crear Centros especializados por cultivos con ámbito de actuación nacional, b cambio en la planificación y gestión de la investigación mediante la creación de Programas Nacionales por cultivos y c formación de 200 investigadores en laboratorios internacionales de prestigio en temas prioritarios para las investigaciones a realizar en los Centros. El presupuesto global del Proyecto fue de 28,2 millones de dólares (equivalentes a unos 204 millones de euros actuales, de los que el BIRF aportó 12,7 millones de dólares en forma de crédito. El proyecto cumplió todos los objetivos fundamentales

  18. Sigma A recognition sites in the Bacillus subtilis genome

    DEFF Research Database (Denmark)

    Jarmer, Hanne Østergaard; Larsen, Thomas Schou; Krogh, Anders Stærmose;

    2001-01-01

    A hidden Markov model of sigma (A) RNA polymerase cofactor recognition sites in Bacillus subtilis, containing either the common or the extended -10 motifs, has been constructed based on experimentally verified sigma (A) recognition sites. This work suggests that more information exists at the ini......A hidden Markov model of sigma (A) RNA polymerase cofactor recognition sites in Bacillus subtilis, containing either the common or the extended -10 motifs, has been constructed based on experimentally verified sigma (A) recognition sites. This work suggests that more information exists...

  19. EVALUACIÓN DE Bombus dahlbomii (GUÉR.) COMO AGENTE POLINIZADOR DE FLORES DE TOMATE (Lycopersicon esculentum (MILL)), BAJO CONDICIONES DE INVERNADERO Evaluation of Bombus dahlbomii (Guér.) as a pollinating agent for tomato flowers under greenhouse conditions

    OpenAIRE

    Patricia Estay P.; Adrian Wagner V.; Moisés Escaff G.

    2001-01-01

    Durante el período estival de 1998, en el Centro Regional de Investigación La Platina, perteneciente al Instituto de Investigaciones Agropecuarias (INIA) (33º 34’ lat. Sur, 70º 38’ long. Oeste), se evaluó el abejorro nativo de Chile Bombus dahlbomii (Guér) como agente polinizador del tomate cultivado Lycopersicon esculentum (Mill). El material entomológico se obtuvo a partir de nidos naturales, los cuales se reinstalaron en colmenas artificiales. Los abejorros fueron liberados en un invernade...

  20. Hemodializuojamų pacientų slaugos ypatumai pacientų ir slaugytojo požiūriu Kauno medicinos universiteto klinikų Detoksikacijos skyriaus duomenimis

    OpenAIRE

    Masiulionytė, Vaida

    2007-01-01

    Darbo tikslas: įvertinti hemodializuojamų pacientų slaugos ypatumus. Metodika. Tyrimo metu Kauno medicinos universiteto klinikų detoksikacijos skyriuje atlikta anketinė tiriamųjų apklausa. Naudotos dvi anoniminės anketos. Viena anketa buvo skirta hemodialize gydomiems pacientams, kita - slaugytojoms. Anketoje pateikti klausimai skirti įvertinti tiriamųjų sociodemografinius duomenis, nuomonę apie pacientų žinias apie ligą, miegą, prisitaikymą prie pasikeitusio gyvenimo režimo, sveikatos pro...

  1. Fluctuación diurna del contenido de vitamina C en hojas de Myrciaria dubia “camu camu”

    OpenAIRE

    Franz Correa Meléndez; Marianela Cobos Ruiz; Roberson Ramirez Saavedra; Sixto Imán Correa; Juan Castro Gómez

    2013-01-01

    Myrciaria dubia “camu camu” es un frutal del trópico amazónico caracterizado por sus frutos con gran contenido de vitamina C, siendo considerado un producto importante del país. Sin embargo, hay pocos reportes sobre el metabolismo y transporte de vitamina C en esta especie. El objetivo de la investigación fue determinar la fluctuación diurna del contenido de vitamina C en hojas de M. dubia “camu camu”. Las hojas se colectaron de la colección de germoplasma de “camu camu” del INIA a las 2, 6, ...

  2. El CITA estudia la importancia de la alimentación en rumiantes

    OpenAIRE

    Area de Información, Documentación y Cultura Científica. Centro de Investigación y Tecnología Agroalimentaria de Aragón

    2014-01-01

    El Centro de Investigación y Tecnología Agroalimentaria de Aragón, organismo adscrito al Departamento de Industria e Innovación del Gobierno de Aragón, trabaja en el proyecto “Efecto de compuestos fenólicos de la dieta sobre los parámetros productivos, reproductivos y de calidad de la canal y de la carne en la especie ovina”, financiado por el Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA).

  3. Biochemistry Identification and Drug Sensitivity Test of Pathogenic Streptococcus from Tilapia in Guangxi%广西罗非鱼链球菌病病原的生化鉴定及药敏试验

    Institute of Scientific and Technical Information of China (English)

    李波; 陈明; 李莉萍; 陈汉忠; 徐增辉; 李超; 黄维义; 梁万文

    2008-01-01

    从来自南宁、北海等地的十几个暴发疾病的罗非鱼养殖场发病罗非鱼中,分离得到了8株链球菌.通过生化鉴定及药敏试验,结果表明,分离到的8株致病性链球菌,有6株为海豚链球菌(streptococcus iniae),2株为无乳链球菌(streptococcus agalactiae).

  4. 常见病害防治方法

    Institute of Scientific and Technical Information of China (English)

    2008-01-01

    @@ 罗非鱼链球菌病 病原:无乳链球菌(Streptococcus agalactiae),海豚链球菌(Streptococcus iniae)也可以引起罗非鱼大量死亡,但危害程度不如无乳链球菌.链球菌是一种革兰氏阳性、非酸性、无运动氧化酶阳性、过氧化氢酶阳性的菌.

  5. A doctoral thesis about Carol Davila written in Paris in 1936.

    Science.gov (United States)

    Rogozea, Liliana; Dumitrascu, Dinu I; Triff, Dorin; Leasu, Florin; Dumitraşcu, Dan L

    2014-01-01

    Carol Davila, the father of the Romanian modern medicine, made decisive contributions to the development of health sciences in the Romanian Principalities in the last decades of the nineteenth century. The merit of his scientific work was recognized beyond the borders of his country. His life (not devoid of anecdotic instances and unknown episodes) and especially his work have aroused considerable interest among numerous medical historians. This paper presents a historical study elaborated in France, but until recently ignored, dedicated to the biography of Carol Davila. It concerns the medical doctoral thesis (State Diploma) elaborated by Joseph Adler (born 1910 in Botoşani, Romania) under the supervision of professor Maxime Laignel-Lavastine, whose interest in Romania is well-known. Professor Laignel-Lavastine held the office of Secretary General of the International Society of History of Medicine (ISHM), founded in 1921, (replaced in this position by another French professor with links to Romania: Jules Guiart). The thesis comprises 48 pages and an exhaustive bibliography. It represents a token of the appreciation given to Davila's achievements by Europe's medical community.

  6. Caracteristici ale procesului decizional la nivel regional. De la caracteristici la eficienţă administrativă (Features of decizion-making process at the regional level. From features to administrative efficiency

    Directory of Open Access Journals (Sweden)

    Dorina ŢICU

    2014-02-01

    Full Text Available The decision-making process et the administrative level is the mechanism through which the decisions are taken. It is a laborious process that encompasses a multitude of dimensions that overlap the public policies cycle with all its stages (problem definition, choosing alternatives, decision-making process, implementation and evaluation of the decision implemented and that ensures in a less or a greater manner the administrative efficiency, especially in a context of decentralization of this sector or of regionalization. From this point of view, the role of this article is to identify, on the basis of a quantitative study conducted at the level of the administration of the Region of Moldavia, on the city-halls of Botoşani, Iaşi, Bacău, Suceava, Vaslui, Piatra Neamt, Focsani, Galati, the main features of the decision-making process and to place them under an integrated model in order to try to satisfy the principle of efficiency. Outlining such a model has a role in both scientific and comprehensive, but also of good practice-integrator for the various administrative centres in the context of the requirements imposed by the process of regionalization.

  7. Percepţii ale inginerilor silvici asupra vulnerabilităţilor şi riscurilor ecosistemelor forestiere în contextul schimbărilor climatice [Perceptions of forestry engineers on vulnerabilities and risks of forest ecosystems to climate change

    Directory of Open Access Journals (Sweden)

    Mihaela Mutu

    2014-08-01

    Full Text Available A growing recent literature argues that the adaptation to the climate change depends on the perceptions that the stakeholders have on potential effects of the climate change and the possibilities to counteract these effects. This study focuses on forestry engineers’ perceptions on climate changes and adapting measures. A number of 76 semi-structured interviews were conducted with forest engineers from three counties in North-Eastern Romania (Suceava, Neamţ and Botoşani. The results show that the forest engineers perceived as climate change-related vulnerabilities: the occurrence of drought, the risk of increased windfall, and the insects attacks. However, the climate change-related risk is ranked only as a fifth threat on forest ecosystems stability, far behind the political control over forest administration and law implementation, incoherence of the legislative frame, un-appropriate legislation for private forests and illegal logging. We conclude that climate change adaptation is not a priority of forest management, which is correlated with the fact that more than half of the respondents estimates the impact of climate change on forests as being small or moderate, while one third of the respondents clearly manifest the preference for non-adapting behavior.

  8. A doctoral thesis about Carol Davila written in Paris in 1936.

    Science.gov (United States)

    Rogozea, Liliana; Dumitrascu, Dinu I; Triff, Dorin; Leasu, Florin; Dumitraşcu, Dan L

    2014-01-01

    Carol Davila, the father of the Romanian modern medicine, made decisive contributions to the development of health sciences in the Romanian Principalities in the last decades of the nineteenth century. The merit of his scientific work was recognized beyond the borders of his country. His life (not devoid of anecdotic instances and unknown episodes) and especially his work have aroused considerable interest among numerous medical historians. This paper presents a historical study elaborated in France, but until recently ignored, dedicated to the biography of Carol Davila. It concerns the medical doctoral thesis (State Diploma) elaborated by Joseph Adler (born 1910 in Botoşani, Romania) under the supervision of professor Maxime Laignel-Lavastine, whose interest in Romania is well-known. Professor Laignel-Lavastine held the office of Secretary General of the International Society of History of Medicine (ISHM), founded in 1921, (replaced in this position by another French professor with links to Romania: Jules Guiart). The thesis comprises 48 pages and an exhaustive bibliography. It represents a token of the appreciation given to Davila's achievements by Europe's medical community. PMID:26527997

  9. Indexes and efficiencies of N optimum dose reviewed as water- and Nitrogen- footprint

    Science.gov (United States)

    Castellanos, Maria Teresa; Cartagena, Maria Carmen; Cabello, Maria Jesus; Rivas, Francisco; Tarquis, Ana Maria; Arce, Augusto

    2013-04-01

    In order to establish rational nitrogen (N) fertilization and reduce groundwater contamination, a clearer understanding of the N distribution through the growing season and its balance is crucial. In three successive years, a melon crop (Cucumis melo L. cv. Sancho) was grown under field conditions to determine the uptake of N fertilizer, applied by means of fertigation at different stages of plant growth. In addition, Strategies are being sought to increase water use in cropping systems and to reduce drainage. The estimation of N mineralized from soil organic matter is an essential tool to determine the amount necessary to optimize crop yield and minimize the environmental impact of excess N. In this study we propose a methodology that allows us to study fertigated management integrating several aspects: economic and environmental. Even the complexity of the system, we have reduced the number of indexes and efficiencies need to establish the framework of N management and its economical and environmental consequences. At the same time, we have translated all them into a water- and Nitrogen- footprint in each year. ACKNOWLEDGEMENTS This work has been partially supported by INIA under Project INIA-RTA 2010-00110-C03-02

  10. Identification and expression analysis of TLR2 in mucosal tissues of turbot (Scophthalmus maximus L.) following bacterial challenge.

    Science.gov (United States)

    Liu, Fengqiao; Su, Baofeng; Gao, Chengbin; Zhou, Shun; Song, Lin; Tan, Fenghua; Dong, Xiaoyu; Ren, Yichao; Li, Chao

    2016-08-01

    The pathogen recognition receptors (PRRs), which can recognize the conserved pathogen-associated molecular patterns (PAMPs) of the bacteria, play key roles in the mucosal surfaces for pathogen recognition and activation of immune signaling pathways. However, our understanding of the PRRs and their activities in mucosal surfaces in the critical early time points during pathogen infection is still limited. Towards to this end, here, we sought to identify the Toll-like receptor 2 (TLR2) in turbot as well as its expression profiles in mucosal barriers following bacterial infection in the early time points. The full-length TLR2 transcript consists of open reading frame (ORF) of 2451 bp encoding the putative peptide of 816 amino acids. The phylogenetic analysis revealed the turbot TLR2 showed the closest relationship to Paralichthys olivaceus. The TLR2 mRNA expression could be detected in all examined tissues, with the most abundant expression level in liver, and the lowest expression level in skin. In addition, TLR2 showed different expression patterns following Vibrio anguillarum and Streptococcus iniae infection, but was up-regulated following both challenge, especially post S. iniae challenge. Characterization of TLR2 will probably contribute to understanding of a number of infectious diseases and broaden the knowledge of interactions between host and pathogen, which will eventually help in the development of novel intervention strategies for farming turbot. PMID:27368539

  11. Efficiency and environmental indexes to evaluate the sustainability of mineral and organic fertilization in an irrigated melon crop

    Science.gov (United States)

    Requejo Mariscal, María Isabel; Villena Gordo, Raquel; Cartagena Causapé, María Carmen; Arce Martínez, Augusto; Ribas Elcorobarrutia, Francisco; Jesús Cabello Cabello, María; María Tarquis Alfonso, Ana; Castellanos Serrano, María Teresa

    2014-05-01

    Melon is traditionally cultivated in fertigated farmlands in the center of Spain with high inputs of water and N fertilizer. Excess N can have a negative impact, from the economic point of view, since it can diminish the production and quality of the fruit, from the environmental point of view, since it is a very mobile element in the soil and can contaminate groundwater. From health point of view, nitrate can be accumulated in fruit pulp, and, in addition, groundwater is the fundamental supply source of human populations. Best management practices are particularly necessary in this region as many zones have been declared vulnerable to NO3- pollution (Directive 91/676/CEE) During successive years, a melon crop (Cucumis melo L.) was grown under field conditions applying mineral and organic fertilizers under drip irrigation. Different doses of ammonium nitrate were used as well as compost derived from the wine-distillery industry which is relevant in this area. The present study reviews the most common N efficiency indexes [1] under the different management options with a view to maximizing yield and minimizing N loss. Acknowledgements: This project has been supported by INIA-RTA04-111-C3 and INIA-RTA2010-00110-C03-01. [1] Castellanos, M., Tarquis, A., Ribas, F., Cabello, M., Arce, A., & Cartagena, M. (2013). Nitrogen fertigation: An integrated agronomic and environmental study. Agricultural Water Management, 120, 46-55.

  12. Transmission and pathology of Streptococcus inane in monosex Nile tilapia (Oreochromis niloticus in aquaculture of Bangladesh

    Directory of Open Access Journals (Sweden)

    Md. Mer Mosharraf Hossain

    2014-04-01

    Full Text Available Streptococcus iniae is a major fish pathogen, recently emergent outbreaks were recorded in commercially cultured monosex Nile tilapia (Oreochromis niloticus result in significant losses termed “streptococcosis”-causes unusual appearances with multi-focal pin-point haemorrhages, abscesses, necrosis and ascites in skin, fin, muscle, liver, spleen, kidney, blood, interstitial fluid specially in central nervous system and brain. This disease was more prevalent (>26% at summer when the water temperature was approximately >25oC, percentage of mortality was higher >41% during the overcrowding and improper water chemistry. Raised levels of glucose and ammonium in blood serum causes reduced number of free blood cells released into the haemolymph to stomach and gut, result in refrain from eating in diseased tilapia. Stocking density (200 fish/decimal; class IV had significant effect (P<0.01 on the total production (5,000 to 5,500 kg/ha. S. iniae in the circulating blood cells, extra-tubular haemal spaces containing blood vessels, fixed phagocytes in the hepatopancreas (gastrointestinal tract, bacteria-like particles in the brain tissue, vacuum and necrosis in hepatocytes revealed with histopathology. In vitro study revealed that cohabitation of dead or infected fish with healthy fish resulted infection (horizontal transmission mechanism to the healthy fish.

  13. A prebiotic role of Ecklonia cava improves the mortality of Edwardsiella tarda-infected zebrafish models via regulating the growth of lactic acid bacteria and pathogen bacteria.

    Science.gov (United States)

    Lee, WonWoo; Oh, Jae Young; Kim, Eun-A; Kang, Nalae; Kim, Kil-Nam; Ahn, Ginnae; Jeon, You-Jin

    2016-07-01

    In this study, the beneficial prebiotic roles of Ecklonia cava (E. cava, EC) were evaluated on the growth of lactic acid bacteria (LAB) and pathogen bacteria and the mortality of pathogen-bacteria infected zebrafish model. The result showed that the original E. cava (EC) led to the highest growth effects on three LABs (Lactobacillus brevis, L. brevis; Lactobacillus pentosus, L. pentosus; Lactobacillus plantarum; L. plantarum) and it was dose-dependent manners. Also, EC, its Celluclast enzymatic (ECC) and 100% ethanol extracts (ECE) showed the anti-bacterial activities on the fish pathogenic bacteria such as (Edwardsiella tarda; E. tarda, Streptococcus iniae; S. iniae, and Vibrio harveyi; V. harveyi). Interestingly, EC induced the higher production of the secondary metabolites from L. plantarum in MRS medium. The secondary metabolites produced by EC significantly inhibited the growth of pathogen bacteria. In further in vivo study, the co-treatment of EC and L. plantarum improved the growth and mortality of E. tarda-infected zebrafish as regulating the expression of inflammatory molecules such as iNOS and COX2. Taken together, our present study suggests that the EC plays an important role as a potential prebiotic and has a protective effect against the infection caused by E. tarda injection in zebrafish. Also, our conclusion from this evidence is that EC can be used and applied as a useful prebiotic. PMID:27192145

  14. EFFECT OF NITROGEN-FIXING BACTERIA ON GRAIN YIELD AND DEVELOPMENT OF FLOODED IRRIGATED RICE

    Directory of Open Access Journals (Sweden)

    AMAURI NELSON BEUTLER

    2016-01-01

    Full Text Available This study aimed at evaluating the effect of Azospirillum brasilense , a nitrogen - fixing bacterium, on flooded irrigated rice yield. Evaluations were carried out in a shaded nursery, with seedlings grown on an Alfisol. Were performed two sets of experiments. In the first, were carried out four experiments using the flooded rice cultivars INIA Olimar, Puitá Inta - CL, Br Irga 409 and Irga 424; these trials were set up as completely randomized design in a 5x4 factorial scheme, with four replications. Treatments consisted of five nitrogen rates (0, 40, 80, 120 and 160 kg ha - 1 and four levels of liquid inoculant Ab - V5 and Ab - V6 - A. brasilense (0, 1, 2 and 4 times the manufacturer's recommendation without seed treatment. In second set, were performed two experiments using the cultivars Puitá Inta - CL and Br Irga 409, arranged in the same design, but using a 4x2 factorial. In this set, treatments were composed of four levels of Ab - V5 and Ab - V6 - A. brasilense liquid inoculant (0, 1, 2 and 4 times the recommendation of 100 mL ha - 1 , using rice seeds with and without insecticide and fungicide treatment. Shoot dry matter, number of panicles, and rice grain yield per pot were the assessed variables. The results showed that rice seed inoculation with A. brasilense had no effects on rice grain yield of the cultivars INIA Olimar, Puitá Inta - CL, Br Irga 409 and Irga 424.

  15. Dietary supplementation of cumin (Cuminum cyminum preventing streptococcal disease during first-feeding of Mozambique tilapia (Oreochromis mossambicus

    Directory of Open Access Journals (Sweden)

    Sevdan Yılmaz

    2013-01-01

    Full Text Available This study was conducted to investigate the effect of dietary cumin (Cuminum cyminum powder (CP as a feed additive on growth performance and disease resistance during first-feeding of Mozamique tilapia (Oreochromis mossambicus. Five isonitrogenous (40% crude protein and isocaloric (18.9 kj g-1 diets were formulated to contain 0 (control, 0.5, 1, 1.5, and 2.0% CP. In a 45-day feeding trial, 15 plastic tanks (21 L were stocked with 40 fry (0.012 ± 0.001 g each. After feeding experiment, fish were infected with Streptococcus iniae and mortalities were recorded. The second-order polynomial regression indicated that a dietary CP level of 1.14% provided the best survival rate challenge infection with S. iniae, growth performance and feed utilization. In conclusion, CP can be used as growth promoter to improve feed utilization and weight gain in tilapia fry, and it can be also used as an antimicrobial agent during first-feeding of O. mossambicus. Therefore, CP can be suggested as an alternative to antibiotics in controlling streptococcal disease in tilapia culture.

  16. Genomic characterization, expression analysis, and antimicrobial function of a glyrichin homologue from rock bream, Oplegnathus fasciatus.

    Science.gov (United States)

    Kasthuri, Saranya Revathy; Wan, Qiang; Umasuthan, Navaneethaiyer; Bathige, S D N K; Lim, Bong-Soo; Jung, Hyung-Bok; Lee, Jehee; Whang, Ilson

    2013-11-01

    Antimicrobial peptides are important innate effector molecules, playing a vital role in antimicrobial immunity in all species. Glyrichin is a transmembrane protein and an antibacterial peptide, exerting its functions against a wide range of pathogenic bacteria. In this study, cDNA and a BAC clone harboring the glyrichin gene were identified from rock bream and characterized. Genomic characterization showed that the OfGlyrichin gene exhibited a 3 exon-2 intron structure. OfGlyrichin is a 79-amino-acid protein with a transmembrane domain at (22)GFMMGFAVGMAAGAMFGTFSCLR(44). Pairwise and multiple sequence alignments showed high identity and conservation with mammalian orthologues. Phylogenetic analysis showed a close relationship with fish species. Higher levels of OfGlyrichin transcripts were detected in the liver from healthy rock bream which were induced by immunogens like lipopolysaccharide, poly I:C, rock bream irido virus, Edwardsiella tarda and Streptococcus iniae. The synthetic peptide (pOf19) showed antibacterial activity against Escherichia coli, E. tarda, and S. iniae. Analysis of the bacterial morphological features after pOf19 peptide treatment showed breakage of the cell membrane, affirming that antibacterial function is accomplished through membrane lysis. The pOf19 peptide also showed antiviral activity against RBIV infection. The high conservation of the genomic structure and protein, together with the antimicrobial roles of OfGlyrichin, provide evidence for the evolutionary existence of this protein playing a vital role in innate immune defense in rock bream.

  17. The influence of seasonality, tide and time of activities on the behavior of Sotalia guianensis (Van Bénéden (Cetacea, Delphinidae in Pernambuco, Brazil Influência da sazonalidade, maré e horário de atividades sobre o comportamento de Sotalia guianensis (Van Bénéden (Cetacea, Delphinidae em Pernambuco, Brasil

    Directory of Open Access Journals (Sweden)

    Janaina P. Araújo

    2007-12-01

    écie em ocupar ambientes mais protegidos, sendo o porto do Recife o principal ponto de concentração da espécie. Piedade é apenas uma área exploratória, possivelmente pela ocorrência de tubarões. Olinda tem uso ocasional. As áreas de concentração parecem estar destinadas à alimentação, e a incidência de condutas caudais sugere que a maioria das presas dos botos-cinza é demersal. As condições ambientais (sazonalidade, maré e horário não se mostraram significativas quando relacionadas com as condutas realizadas pelos botos-cinza.

  18. Mechanical characterization of sawn timber for structural use, graded visually in accordance with Spanish standard UNE 56544

    Directory of Open Access Journals (Sweden)

    Fernández-Golfín, J. I.

    1998-12-01

    Full Text Available The employment of timber in structures in Spain was hurting of the nonexistence of accepted calculation procedures. The recent adoption and publication of the Eurocode 5 compels to the employment of the characteristic values of the material in the structural calculation by the method of the finite elements. This report sets forth the results of the experiments carried out at INIA's Forest Research Centre (CIFOR-INIA regarding the mechanical characterization of sawn timber of size 150x50x3.000 mm and the structural uses of "Pinus sylvestris, P. radiata and P. pinaster" from different Spanish provenances. The characteristic values and strength classes corresponding with the grades stated in the Spanish standard UNE 56544 are also provided. The report compares and analyses the results of these experiments, and presents the conclusions drawn from the different behaviour shown by the four species. An analysis of the Spanish standard for the visual grading of structural timber is carried out on the three species.

    El empleo de la madera en la construcción en España adolecía de la inexistencia de procedimientos de cálculo normalizados. La reciente adopción y publicación del Eurocódigo 5 obliga al empleo de los valores característicos del material en el cálculo estructural de la madera por el método de los elementos finitos. Se presentan los resultados de la caracterización mecánica de la madera aserrada de tamaño (150x50x3.000 mm y usos estructurales de "Pinus sylvestris, P. radiata y P. pinaster" de diferentes procedencias, llevada a cabo en el Centro de Investigación Forestal (CIFOR del INIA. Se aportan los valores característicos y clases de resistencia que corresponden a las clases de calidad definidas en la norma UNE 56544. Se efectúa una comparación y análisis de los resultados obtenidos, extrayendo conclusiones respecto del diferente comportamiento observado para las cuatro especies. Se analiza la adecuación de la

  19. Identification of Sand flies of the Subgenus Larroussius based on Molecular and Morphological Characters in North Western Iran

    Directory of Open Access Journals (Sweden)

    A Absavaran

    2009-12-01

    Full Text Available Background: The adult female sand flies (Diptera: Psychodidae of the subgenus Larroussius are important vectors of Leishmania infantum (Kinetoplastida: Tripanosomatidae in Meshkinshahr district, Northwest of Iran. Four Phle­boto­mus (Larroussius species are present in this area, i.e. Phlebotomus (Larroussius kandelakii, P. (La. major, P. (La. perfiliewi and P. (La. tobbi. The objective of the present study was to identify and distinguish the females of P. per­filiewi, P. major and P. tobbi, in this district.Methods: Adult sand flies were collected with sticky papers, CDC light traps, and aspirator in 2006. Individual sand flies of this four species from thirty different locations were characterized morphologically and by comparative DNA se­quences analyses of a fragment of mitochondrial gene Cytochrome b (Cyt b and nuclear gene Elongation Factor 1- al­pha (EF-1α. PCR amplification was carried out for all three species P. major, P. perfiliewi and P. tobbi in the sub­ge­nus Larroussius.Results: Phylogenetic analyses of P. major populations in this study displayed two different populations and genetic di­ver­sity. Spermathecal segment number, pharyngeal armature and other morphological characters of these three spe­cies were examined and found to present consistent interspecific differences.Conclusion: According to our findings, the phylogeny of Cyt b and EF-1α haplotypes confirms the relationships be­tween P. major, P. tobbi and P. perfiliewi as already defined by their morphological similarities.

  20. Maisto reklama ir ženklinimas

    OpenAIRE

    Kornyšova, Svetlana

    2010-01-01

    Maisto reklamos ir ženklinimo tyrimas buvo atliekamas, tikslu išsiaiškinti ir įvertinti Lietuvos gyventojų žinias apie maisto produktų ženklinimą, jo trūkumus bei apie reklamuojamų maisto produktų pasirinkimą. Darbo uždaviniai: 1. ištirti ir įvertinti vartotojų supratimą apie maisto produktų etiketėse pateiktą ženklinimo informaciją; 2. ištirti ir įvertinti respondentų nuomonę apie maisto produktų etikečių įskaitomumą; 3. ištirti ir įvertinti maisto produktų reklamos įtaką jų pasirinkimui. Ty...

  1. Korporatyvinės įmonės duomenų saugyklos modelio sudarymas ir tyrimas

    OpenAIRE

    Buškauskaitė, Laima

    2007-01-01

    Šiuolaikinis verslas naudoja didžiulį duomenų kiekį, tačiau įmonėje šie duomenys taip ir liks tik balastas, jeigu nesugebėsime jų išanalizuoti ir tinkamai interpretuoti. Tik duomenų analizė, naudojant specialius programinius įrankius, iš „žalios“ informacijos leis atrinkti naudingus grūdelius ir perdirbti juos į vertingas žinias, kuriuos taps teisingų verslo sprendimų pagrindu. Naudojant OLAP (On-line Analytical Processing) priemones, sukuriama duomenų saugykla, kuri leidžia greitai, bei pato...

  2. Farmacininkų požiūrio į GMO vertinimas

    OpenAIRE

    Našlėnė, Žilvinė

    2014-01-01

    Tyrimo tikslas – įvertinti vaistinių darbuotojų požiūrį į GMO. Tikslo įgyvendinimui iškelti 3 uždaviniai – įvertinti vaistinių darbuotojų žinias apie GMO, įvertinti vaistinių darbuotojų požiūrį į GMO riziką aplinkai ir žmonių sveikatai bei gautus rezultatus palyginti su 2009 m. ”Spinter“ visuomenės požiūrio į GMO tyrimais. Metodika. Atlikta anoniminė vaistinų darbuotojų anketinė apklausa. Apklausta 150 respondentų: 75 iš Vilniaus miesto ir 75 iš Ukmergės ir Šakių miestų. Duomenų statistinė an...

  3. Influence of Conservation Tillage and Soil Water Content on Crop Yield in Dryland Compacted Alfisol of Central Chile Influencia de la Labranza de Conservación y el Contenido de Agua sobre el Rendimiento del Cultivo en un Alfisol compactado del Secano Central de Chile

    Directory of Open Access Journals (Sweden)

    Ingrid Martinez G

    2011-12-01

    Full Text Available Chilean dryland areas of the Mediterranean climate region are characterized by highly degraded and compacted soils, which require the use of conservation tillage systems to mitigate water erosion as well as to improve soil water storage. An oat (Avena sativa L. cv. Supernova-INIA - wheat (Triticum aestivum L. cv. Pandora-INIA crop rotation was established under the following conservation systems: no tillage (Nt, Nt + contour plowing (Nt+Cp, Nt + barrier hedge (Nt+Bh, and Nt + subsoiling (Nt+Sb, compared to conventional tillage (Ct to evaluate their influence on soil water content (SWC in the profile (10 to 110 cm depth, the soil compaction and their interaction with the crop yield. Experimental plots were established in 2007 and lasted 3 yr till 2009 in a compacted Alfisol. At the end of the growing seasons, SWC was reduced by 44 to 51% in conservation tillage systems and 60% in Ct. Soil water content had a significant (p En Chile, las zonas de clima mediterráneo se caracterizan por suelos altamente degradados y compactados por erosión, lo que requiere el uso de sistemas de labranza conservacionista para mitigar la erosión hídrica, así como incrementar el contenido de agua en el suelo. Se evaluó una rotación avena (Avena sativa L. cv. Supernova-INIA - trigo (Triticum aestivum L. cv. Pandora-INIA establecida bajo los siguientes sistemas conservacionistas: cero labranza (Nt, Nt + curvas de nivel (Nt+Cp, Nt + franjas vivas (Nt+Bh y Nt + subsolado (Nt+Sb, las que fueron comparadas al sistema de labranza convencional (Ct, para evaluar su influencia en el contenido de agua en el suelo (SWC en el perfil (10 a 110 cm profundidad, la compactación del suelo y su interacción con el rendimiento del cultivo. Las parcelas experimentales fueron establecidas 3 años seguidos (2007 al 2009 en un Alfisol compactado. Al final de la temporada, el SWC disminuyó 44 a 51% en los sistemas conservacionistas y 60% en el sistema convencional. El sistema de

  4. Analysis of Genetic Variability among thirty accessions of Andean Lupin (Lupinus mutabilis Sweet using ISSR molecular markers

    Directory of Open Access Journals (Sweden)

    Michelle C. Chirinos-Arias

    2015-03-01

    Full Text Available In order to make the genetic variability analysis among thirty accessions of andean lupine (L. mutabilis Sweet belonging to Agrarian Innovation National Institute (INIA Seed Bank. DNA was extracted from 300 plants and we made bulks. We standardized amplification protocol of Inter Simple Sequence Repeat (ISSR primers, we chose the most polymorphic primers to run in acrylamide gel. We found 255 ISSR loci with 8 primers. It was found high genetic variability of the samples under study by ISSR markers. Also observed relatively high polymorphism for autogamous species such as andean lupine. Finally phenograms showed a relationship with the geographical location, possibly due to in situ gene flow due to the exchange or sale of seeds in markets near the collection area.

  5. Ekologinio ūkio ūkininkų požiūrio į genetiškai modifikuotus produktus ir jų galimą riziką žmonių sveikatai įvertinimas

    OpenAIRE

    Šimkienė, Oksana

    2010-01-01

    Darbo tikslas: buvo ištirti ekologinių ūkių ūkininkų žinias ir požiūrį į genetiškai modifikuotus organizmus, jų produktus bei galimą riziką aplinkai ir žmonių sveikatai. Metodika: tyrime dalyvavo atsitiktinai atrinkti Lietuvoje sertifikuotų ekologinių ūkių ūkininkai, 18 metų ir vyresni nei 70, turintys įvairią ūkininkavimo patirtį. Apklausoje dalyvavo 188 vyrai ir 114 moterų. Vertinome, kaip atsakymai priklausė nuo ūkininkų amžiaus, ūkininkavimo patirties ir lyties. Skirtumą laikėme rei...

  6. Paauglių merginų fizinį savivaizdį formuojantys veiksniai

    OpenAIRE

    Žukauskaitė, Andželika

    2012-01-01

    Pastaruoju metu mokslininkų tarpe vis labiau domimasi kūno įvaizdžiu. Tai nulėmė visuomenėje įsivyravę aukšti tobulo kūno standartai, kurių siekimas žmonėms tampa vis svarbesnis. Vis didesnį neramumą kelia paauglių požiūris į save ir santykį su išvaizda. Pastebima, kad jie yra linkę save vertinti kritiškai, vis dažniau yra nepatenkinti savo išvaizda. Įvairūs mokslininkai (Druxman, 2003; Grogan, 2008; Pruskus, 2008) pripažįsta neabejotiną socialinių veiksnių (šeimos, draugų, bendraamžių, žinia...

  7. STUDIES ON PHYSIOLOGICAL AND BIOCHEMICAL RESPONSES OF ROBINIA PSEUDOA CA CIA CLONES UNDER WATER STRESS%水分胁迫下刺槐不同无性系生理生化反应的研究

    Institute of Scientific and Technical Information of China (English)

    张莉; 续九如

    2003-01-01

    Water stress can cause all kinds of physiological and biochemical responses on forest tree, which are indicated by changes of many physiological and biochemical indexes. Under the different drought extent of soil, the seedlings of Rob-inia pseudoacacia clones were tested. Testing indexes included water content, the ratio of bound water content and free wa-ter content ( Vα/Vs), free proline, soluble sugar, soluble protein ect. The results showed that water content decreased with the increase of soil drought, the rest indexes increased in different extent. The range of variance differed among clones. It was difficult to evaluate exactly the drought resistance of different clones depending on only physiological and biochemical index.

  8. Production of camu camu plants with different organic substrates in conventional nursery bed

    Directory of Open Access Journals (Sweden)

    Carlos Abanto Rodriguez

    2013-12-01

    Full Text Available The aim was to verify the initial development of camu camu plants with different organic substrates in conventional nursery bedding microsprinkler irrigation and shade management Chromatine® mesh with 50% red light. We used genetic material from mother plants Germplasm Bank of INIA-camu camu Iquitos, seeds were germinated in decomposed sawdust and kept for a period of 40 days after subculturing was performed with 10 cm in height in different substrates according treatments in nursery beds with dimensions of 1.20 m wide x 10 m long with a depth of 30 cm. After conducting assessments of height (cm and basal diameter (mm for a period of 120 days, it was found that the substrate manure has become the substrate for greater efficiency in plant development camu camu substrate followed by humus worm.

  9. Kaišiadorių apylinkėse tautinėje vaistininkystėje vartojamų (vartotų) augalų etnofarmacinis tyrimas

    OpenAIRE

    Ivaškevičienė, Miglė

    2014-01-01

    Tyrimo tikslas surinkti ir susisteminti tautinės vaistininkystės žinias Kaišiadorių apylinkėse. Rezultatai: Apklausų - interviu metu apklausti 23 respondentai. Užfiksuotos 155 natūralios vaistingosios medžiagos. Didžiausią jų dalį sudarė augalai, kurių įvairovė siekia 62 augalų šeimas, taip pat užfiksuoti 3 gyvūnai, vartojami kaip gydomoji priemonė. Išvados: 1. Anketavimo – asmeninio interviu metu gauta medžiaga susisteminta pagal vaistingųjų medžiagų kilmę. Vyrauja augalinės kilmės vaistingo...

  10. Antimicrobial activity of essential oil of Eucalyptus globulus against fish pathogenic bacteria

    Science.gov (United States)

    Park, Joon-Woo; Wendt, Mitchell

    2016-01-01

    The antibacterial activities of the essential oil of Eucalyptus globulus (EOEG) was determined against 7 fish pathogenic bacteria (Edwardsiella tarda, Streptococcus iniae, S. parauberis, Lactococcus garviae, Vibrio harveyi, V. ichthyoenteri and Photobacterium damselae) obtained from farmed olive flounder. The inhibitory activity was evaluated by three methods: Disc diffusion method, minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC). According to the disc diffusion test, as the concentration of EOEG (5-40 µg) rises, the inhibitory zone increases in size. Compared with amoxicillin, tetracycline and chloramphenicol, EOEG showed similar antibacterial activity. The MIC of EOEG ranged from 7.8 to 125 mg/mL and MBC values ranged from 62 to 250 mg/mL. These results show that EOEG has antimicrobial activity against all seven bacteria, but there was no marked difference between each genus. From these results, it is suggested that EOEG can be used as an antimicrobial agent against fish bacterial diseases in the fish industry.

  11. Specialiųjų poreikių (nežymiai protiškai atsilikusių) VIII klasės moksleivių temos „Žinduoliai“ mokymas

    OpenAIRE

    Tijūnaitytė, Aurelija

    2007-01-01

    Darbe atlikta teorinė specialiosios ir bendrojo lavinimo mokyklos programų, gamtos mokslų mokymo metodų, organizavimo formų, bei vaizdinių gamtos mokymo priemonių analizė. Iškeltos hipotezės: kad gamtos dalyko pamokose panaudojus aktyviuosius mokymo metodus, susietus su grafinėmis užduotimis, mokinių žinios apie žinduolių klasę pagerės; kad temos „Žinduoliai“ turinio įsisavinimas geresnis, kai žinių įtvirtinimo užduotys ne vien tikrina ir įtvirtina žinias, bet ir sudaro galimybę pačiam a...

  12. INFLUENCIA DEL GENOTIPO Y TIPO DE INJERTO EN LA BROTACION DE Myrciaria dubia (H.B.K MC VAUGH “CAMU CAMU”

    Directory of Open Access Journals (Sweden)

    Andy Richard Reátegui Salazar

    2012-12-01

    Full Text Available El camu camu, es una especie frutal nativa, silvestre y cultivada de la región amazónica, cuya importancia radica en el alto contenido de vitamina C, antocianinas y compuestos fenólicos presentes en la pulpa y cáscara de sus frutos. El presente trabajo se llevó a cabo en el Campo Experimental San Miguel, del INIA Loreto, con el objetivo de determinar la influencia del genotipo y el tipo de injerto en la brotación. Se evaluaron tres genotipos promisorios de camu camu (MD-014, MD-015 y MD-017 procedentes de la colección de germoplasma del INIA y tres tipos de injerto (astilla simple, astilla doble y púa; utilizándose como patrón plantones del genotipo MD-020, sobre los cuales se realizaron los injertos. La evaluación de la brotación se realizó a los 105 días después del injerto. Los resultados indican que el mejor tipo de injerto es el de púa, que obtuvo 85% de brotación y las mejores interacciones se lograron en los genotipos MD-017 y MD-015 con el injerto tipo púa; que alcanzaron 90% y 87.5% de brotación, respectivamente. Se concluyó que los mejores resultados se lograron cuando se combinaron el genotipo MD-017 con el injerto tipo púa.

  13. Permanencia en vivero del acodo aéreo de Myrciaria dubia “camu camu” y su efecto en la supervivencia en campo definitivo

    Directory of Open Access Journals (Sweden)

    Jorge Gálvez

    2013-06-01

    Full Text Available El camu camu, es una especie frutal nativa, silvestre y cultivada de la región amazónica, importante el alto contenido de vitamina C, antocianinas y compuestos fenólicos presentes en la pulpa y cáscara de sus frutos. Se propaga en forma convencional por semilla botánica, con la desventaja de producir plantaciones desuniformes producto de la alogamia que presenta la planta; sin embargo la propagación vegetativa se presenta como una alternativa que permite mantener el genotipo intacto y asegura el aprovechamiento del germoplasma valioso. El objetivo del trabajo, fue evaluar el tiempo de permanencia del acodo aéreo de camu camu en vivero, para obtener mayores porcentajes de supervivencia en campo definitivo. El experimento se ejecutó en el Campo Experimental “San Miguel” de la Estación Experimental Agraria San Roque del INIA. Los acodos aéreos fueron realizados en una plantación comercial del INIA, luego de enraizados, se separaron y se trasladaron a campo definitivo o a vivero de acuerdo con los tratamientos en estudio. El diseño utilizado fue de bloques completos al azar con tres repeticiones y cuatro niveles del factor tiempo (0, 1, 2 y 3 meses. La variable principal estudiada fue la supervivencia de los acodos trasplantados. El mejor tratamiento fue cuando el acodo aéreo permaneció 3 meses en vivero con 98,61% de supervivencia, a los 7 meses después del trasplante.

  14. Application of water footprint in a fertirrigated melon crop under semiarid conditions: A review.

    Science.gov (United States)

    Castellanos Serrano, María Teresa; Requejo Mariscal, María Isabel; Villena Gordo, Raquel; Cartagena Causapé, María Carmen; Arce Martínez, Augusto; Ribas Elcorobarrutia, Francisco; Jesús Cabello Cabello, María; María Tarquis Alfonso, Ana

    2015-04-01

    In recent times, there has been a major increase in the use of water and fertilizers in order to increase agricultural production, while at the same time there has increased evidence that aquifers are reducing their water level, enriched by nutrient and degraded as a result of pollution. So best management practices are needed for much of cropped, irrigated and fertirrigated land, to avoid contamination of fresh water and groundwater. The concept of "water footprint" (WF) was introduced as an indicator for the total volume of direct and indirect freshwater used, consumed and/or polluted [1]. The WF distinguishes between blue water (volume of surface and groundwater consumed), green water (rain-water consumed), and grey water (volume of freshwater that is required to assimilate the load of pollutants based on existing ambient water quality standards). This study is focused in calculating the crops WF using a real case of study in a fertirrigated melon crop under semiarid conditions which is principally cultivated in the centre of Spain declared vulnerable zone to nitrate pollution by applying the Directive 91/676/CEE. During successive years, a melon crop (Cucumis melo L.) was grown under field conditions applying mineral and organic fertilizers. Different doses of ammonium nitrate were used as well as compost derived from the wine-distillery industry which is relevant in this area. This application help us to review the different concepts in which is based WF. Acknowledgements: This project has been supported by INIA-RTA04-111-C3 and INIA-RTA2010-00110-C03-01. Keywords: Water footprint, nitrogen, fertirrigation, inorganic fertilizers, organic amendments, winery waste, semiarid conditions. [1] Hoekstra, A.Y. 2003. Virtual water trade. Proceedings of the International Expert Meeting on Virtual Water Trade, Delft, The Netherlands, 12-13 December 2002. Value of Water Research Report Series No. 12, UNESCO-IHE, Delft, The Netherlands.

  15. Seed softening patterns of forage legumes in a temperate/subtropical environment in Uruguay

    Directory of Open Access Journals (Sweden)

    Javier Do Canto

    2013-03-01

    Full Text Available Few studies have been conducted in annual and perennial forage legumes to investigate the development of hardseededness and the subsequent pattern of seed softening in temperate and subtropical regions of South America. Experiments were conducted during 2007 and 2008 in central Uruguay to follow the pattern of seed softening in 35 annual and perennial forage legumes, including three native species of Uruguay and five commercial cultivars. Newly ripened seeds of each plant material were placed in mesh packets on the soil surface in mid-summer. Samples were recovered monthly for germination tests and the proportion of residual hard seeds determined. The native species Adesmia bicolor (Poir. DC., Adesmia securigerifolia Herter, and Ornithopus micranthus (Benth. Arechav., together with Ornithopus pinnatus (Mill. Druce cv. INIA Molles behaved similarly. They showed high levels of initial hard seed from 78% in A. bicolor to 99% in A. securigerifolia and O. pinnatus cv. INIA Molles in 2007; displayed pulses of seed softening, particularly in autumn, and retained moderate levels of residual hard seed for the development of a soil seed bank ranging from 15% in A. bicolor to 49% in O. micranthus. These appear to be desirable characteristics for persistence of forage legumes in subtropical grasslands, both for annual and perennial species. Trifolium repens L. and Lotus corniculatus L. produced few hard seeds, only 2% and 13% respectively were hard after 1-mo in the field and were completely soft by July placing extra reliance on their vegetative propagation for persistence. Materials of L. arenarius Brot. showed pronounced late autumn softening, while materials of L. ornithopodioides L. showed extremely high levels of hardseededness (between 96% and 100% and no softening during the evaluation period, apart from two materials that were completely soft seeded. Mediterranean forage legumes should be properly evaluated in temperate and subtropical regions as

  16. Avances de investigación para la propagación vegetativa por cultivo in vitro de Myrciaria dubia (H.B.K. Mc Vaugh “camu camu”

    Directory of Open Access Journals (Sweden)

    Sergio Pinedo Freyre

    2013-06-01

    Full Text Available El trabajo fue realizado en el Laboratorio de Cultivo de Tejidos Vegetales de la Estación Experimental Agraria San Roque, del INIA Loreto con el objetivo de establecer un protocolo para el cultivo in vitro de segmentos nodales de camu camu" Myrciaria dubia " (H.B.K Mc Vaugh. La fuente de material vegetal, fueron ramas de la parte apical de plantas adultas de la Colección Nacional de camu camu del INIA Loreto, se realizaron ensayos de desinfección, medios de cultivo y diferentes concentraciones de hormonas. El medio de cultivo utilizado fue Murashige & Skoog, MS 1962; más factores constantes 20 g/L sacarosa, 0,25 g/L carbón activado, 7 g/L agar y pH 5,7. Los tratamientos de desinfección que presentaron los mayores porcentajes (100% de supervivencia de segmentos nodales, fueron: Hipoclorito 0.3%  + Chupadera 1 g/L en M&S, M&S ½ y M&S + AIB y Hipoclorito 0,5%  + Chupadera 2 g/L en M&S, M&S ½ y M&S + AIB. El mejor medio de cultivo para la fase de crecimiento in vitro fue M&S + 1,5 mg/L GA3 + 10 mg/L AIA con 72% de supervivencia a los 45 días después de la siembra. El mejor medio de cultivo para la fase de multiplicación fue M&S + 2,5 mg/L BAP + 0,1 mg/L ANA con 80% de supervivencia a los 30 días después del subcultivo.

  17. Calidad de consumo en variedades de boniato

    Directory of Open Access Journals (Sweden)

    Sergio Caballo

    2011-04-01

    Full Text Available El presente trabajo describe una experiencia de degustación de cuatro variedades de boniatos, Beauregard, INIA- Arapey, Morada INTA, y el clon E9227.1, en dos formas de cocción, hervidos y horneados, realizada en INIA y LATU. Dos paneles pilotos de consumidores de ambas instituciones, evaluaron los atributos de sabor, textura, color y agrado general, empleando una escala hedónica de nueve puntos. El aspecto comercial fue evaluado estableciendo el orden de preferencia de las variedades sin cocinar. Se realizaron ensayos fisicoquímicos de fructosa, glucosa, sacarosa y maltosa sobre el material crudo y de materia seca y sólidos solubles totales a las muestras crudas y cocidas. El Morada se diferenció por su alto contenido de materia seca y sólidos solubles totales (SST del material procesado. El análisis estadístico  determinó mayor preferencia en aspecto comercial de Morada y clon E9227.1, como también un mayor valor en sabor y agrado general, tanto en la forma hervida como horneada. El valor obtenido en el atributo de color fue mayor en las variedades de pulpa naranja, Beauregard y clon E9227.1. Estos resultados  podrían tomarse como orientativos de las preferencias de consumidores uruguayos y una referencia para el mejoramiento genético, la producción, el comercio y el consumo. Sería recomendable ajustar medidas analíticas que se correlacionen con las preferencias sensoriales.

  18. Effect of mineral and organic fertilization on grey water footprint in a fertirrigated crop under semiarid conditions.

    Science.gov (United States)

    Castellanos Serrano, María Teresa; Requejo Mariscal, María Isabel; Cartagena Causapé, María Carmen; Arce Martínez, Augusto; Ribas Elcorobarrutia, Francisco; Jesús Cabello Cabello, María; María Tarquis Alfonso, Ana

    2016-04-01

    The concept of "water footprint" (WF) was introduced as an indicator for the total volume of direct and indirect freshwater used, consumed and/or polluted [1]. The WF distinguishes between blue water (volume of surface and groundwater consumed), green water (rain-water consumed), and grey water (volume of freshwater that is required to assimilate the load of pollutants based on existing ambient water quality standards). In semiarid scenarios with low water quality, where the irrigation is necessary to maintain production, green WF is zero because the effective rainfall is negligible. As well as blue WF includes: i) extra consumption or irrigation water that the farmer has to apply to compensate the fail of uniformity on discharge of drips, ii) percolation out of control or salts leaching, which depends on the salt tolerance of the crop, soil and quality of irrigation water, to ensure the fruit yield. The major concern is grey WF, because the irrigation and nitrogen dose have to be adjusted to the crop needs in order to minimize nitrate pollution. This study is focused in assessment mineral and organic fertilization on grey WF in a fertirrigated melon crop under semiarid conditions, which is principally cultivated in the centre of Spain declared vulnerable zone to nitrate pollution by applying the Directive 91/676/CEE. During successive years, a melon crop (Cucumis melo L.) was grown under field conditions. Different doses of ammonium nitrate were used as well as compost derived from the wine-distillery industry which is relevant in this area. Acknowledgements: This project has been supported by INIA-RTA04-111-C3 and INIA-RTA2010-00110-C03. Keywords: Water footprint, nitrogen, fertirrigation, inorganic fertilizers, organic amendments, semiarid conditions. [1] Hoekstra, A.Y. 2003. Virtual water trade. Proceedings of the International Expert Meeting on Virtual Water Trade, Delft, The Netherlands, 12-13 December 2002. Value of Water Research Report Series No. 12

  19. Soil solarization of onion nursery beds with different polyethylene thickness

    International Nuclear Information System (INIS)

    Soil solarization means the complete covering of wet soil to field capacity with transparent polyethylene ultraviolet film during a variable period. Due to the solar energy caught soil temperature rises damaging the seed bank of annual weeds. This technique was evaluated in onion seedlings by INIA- DIGEGRA-FAGRO in three locations during 2005-2006. In 2006-2007 and 2007-2008 the effect of solarization on weed population and seedling quality was studied using UV polyethylene films of both 35 and 80 μm thickness as compared to a non solarized control at INIA Las Brujas. Soil was covered on 12/17/06 and 12/3/07 and temperature was recorded 10 cm depth. Seedbeds 5 m long with four rows were sown with onion cv. Pantanoso del Sauce-CRS on 4/30/07 and 04/16/08. Number of weeds/m2, weeds fresh and dry weight, length, stem diameter, fresh and dry weight of 10 seedlings, seedling health (leaf blight, Botrytis spp.), and nitrate and ammonium in the soil were evaluated after solarization. Non solarized plots had significantly more weed infestation and dry weight compared to the solarized ones, and lower levels of soil nitrate and ammonium. The non solarized control showed significantly higher infestation and dry weight of weeds compared to the solarized plots, and lower nitrate and ammonia content. Solarized treatments were healthier than the non solarized ones. There were no statistically significant differences on weed control or seedling quality between polyethylene thickness, but there were significant differences with respect to the non solarized treatment

  20. Doses e épocas de aplicação de nitrogênio na suscetibilidade do arroz à temperatura baixa na fase reprodutiva Nitrogen doses and timing on rice susceptibility to low temperature in reproductive stage

    Directory of Open Access Journals (Sweden)

    Ramón Méndez Larrosa

    2009-07-01

    Full Text Available A aplicação de nitrogênio (N deve afetar a sensibilidade das plantas de arroz quando submetidas ao frio na fase reprodutiva. Assim, foram instalados dois experimentos em casa de vegetação, no Instituto Nacional de Investigação Agropecuária (INIA, INIA Treinta y Tres, Uruguai, na safra agrícola 2007/08. O primeiro experimento teve o objetivo de determinar o efeito da época e o segundo, o efeito de dose de aplicação de N sobre a suscetibilidade de plantas de arroz irrigado à temperatura baixa na fase reprodutiva. Realizou-se um experimento bifatorial em delineamento inteiramente casualizado (fator A: épocas ou doses de aplicação de N e fator B: aplicação ou não de frio nos dois experimentos, com quatro repetições. No experimento 1, os tratamentos do fator A foram a aplicação de N (30kg ha-1 nos estádios V4, V5, V6, V7, V8, R0, R1+4 dias e a testemunha sem N em cobertura. Nesse experimento, os tratamentos que receberam N de V4 a V8 também receberam mais 30kg ha-1 no estádio R0, e os tratamentos que receberam N nos estádios R0 e R1+4 dias também receberam 30kg ha-1 no estádio V4. No experimento 2, o fator A foram níveis de N de 0, 23, 46 e 69kg ha-1 de N no estádio V4 mais uma aplicação de 23kg ha-1 no estádio R0 e as mesmas doses em R0 com 23kg ha-1 no estádio V4, mais uma testemunha sem aplicação de N nos dois estádios. No tratamento com frio, as plantas foram submetidas a 12,5°C durante sete horas em quatro noites quando o colar da folha bandeira ficou com a mesma altura que o colar da folha anterior. Foram avaliados os seguintes parâmetros: a percentagem de esterilidade de espiguetas, o índice SPAD, realizado aos três, 10 e 16 dias após o tratamento do frio no estádio R2-R3, a produtividade de grãos por vaso, o número de panículas por balde e a massa de mil grãos. A esterilidade de espiguetas não foi influenciada pela época, pela dose de aplicação de N ou pelo tratamento de frio, mas encontrou

  1. Identification of Sand flies of the Subgenus Larroussius based on Molecular and Morphological Characters in North Western Iran

    Directory of Open Access Journals (Sweden)

    A Absavaran

    2009-12-01

    Full Text Available Background: The adult female sand flies (Diptera: Psychodidae of the subgenus Larroussius are important vectors of Leishmania infantum (Kinetoplastida: Tripanosomatidae in Meshkinshahr district, Northwest of Iran. Four Phle­boto­mus (Larroussius species are present in this area, i.e. Phlebotomus (Larroussius kandelakii, P. (La. major, P. (La. perfiliewi and P. (La. tobbi. The objective of the present study was to identify and distinguish the females of P. per­filiewi, P. major and P. tobbi, in this district."nMethods: Adult sand flies were collected with sticky papers, CDC light traps, and aspirator in 2006. Individual sand flies of this four species from thirty different locations were characterized morphologically and by comparative DNA se­quences analyses of a fragment of mitochondrial gene Cytochrome b (Cyt b and nuclear gene Elongation Factor 1- al­pha (EF-1α. PCR amplification was carried out for all three species P. major, P. perfiliewi and P. tobbi in the sub­ge­nus Larroussius."nResults: Phylogenetic analyses of P. major populations in this study displayed two different populations and genetic di­ver­sity. Spermathecal segment number, pharyngeal armature and other morphological characters of these three spe­cies were examined and found to present consistent interspecific differences."nConclusion: According to our findings, the phylogeny of Cyt b and EF-1α haplotypes confirms the relationships be­tween P. major, P. tobbi and P. perfiliewi as already defined by their morphological similarities.                                                                                  Keywords: Phlebotomus, Larroussius, Cytochrome b, Elongation Factor-1α, Morphology, Iran                 

  2. 降低交叉反应的鱼类病原菌检测抗体芯片初步研究%PRELIMINARY STUDY ON REDUCTION OF CROSS-REACTION OF ANTIBODY MICROARRAY FOR DETECTION OF PATHOGENIC BACTERIA IN FISH

    Institute of Scientific and Technical Information of China (English)

    王欣欣; 绳秀珍; 战文斌

    2013-01-01

    采用菌体吸附法对海豚链球菌(Streptococcus iniae)、迟缓爱德华氏菌(Edwardsiella tarda)、鳗弧菌(Vibrio anguillarum)、杀鲑气单胞菌(Aeromonas salmonicida)、荧光假单胞菌(Psedomonas fluorescens)和海洋分支杆菌(Mycobacterium marinum)6种养殖鱼类病原菌的兔抗血清进行吸附,以减小抗血清与其它菌株的交叉反应;吸附纯化后的抗体作为捕获抗体构建了病原菌检测抗体芯片.实验结果显示,与未经过吸附的纯化抗血清构建的抗体芯片相比,血清吸附实验能有效减少交叉反应,降低抗体的非特异性吸附,提高细菌检测的特异性.同时,以迟缓爱德华氏菌为例,采用吸附纯化后抗体制备的抗体芯片检测人工感染迟缓爱德华氏菌和自然发病的牙鲆,均观察到迟缓爱德华民菌的特异性显色反应,得到了较理想的检测结果.本文结果说明优化后的病原菌检测抗体芯片可用于水产养殖鱼类常见的上述6种细菌性疾病的有效检测.%Six kinds of bacterias from marine cultured fish, including Streptococcus iniae , Edwardsiella tarda, Vibrio anguillarum , Aeromonas salmonicida , Psedomonas fluorescens and Mycobacterium ma-rinum , were chosen, and their rabbit antiserum were adsorbed to reduce cross reaction by serum adsorption experiment. And then antibody microarrays were developed using the purified rabbit antiserum as capture antibodies. The results showed that the cross reaction of the antibody microarray was reduced effectively, and the specificity and sensibility of bacteria detection were improved greatly after serum adsorption. Meanwhile, taking E. tarda for example, the antibody microarray was applied to detect E. tarda in Paralichthys olivaceus infected artificially and naturally with E. tarda, and specific positive staining were observed, suggesting the developed antibody microarrays could effectively detect the six kinds of bacteria above-mentioned in fish.

  3. THE NATIONAL STRUCTURE OF THE ROMANIAN POPULATION AT THE 20TH OF OCTOBER 2011 CENSUS

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    GR. P. POP

    2014-06-01

    Full Text Available The National Structure of the Romanian Population at the 20th of October 2011 Census. This is the third study regarding the national structure of the population of Romania. The first two approached the 1992 and 2002 censuses and were published in Studia UBB, Geographia, 2 (1991 and the Romanian Review of Political Geography (20042. On the census day, Romania had 20,121,641 inhabitants, of which 83.45% (16,792,868 are Romanians, while the other 16.55% belong to: Hungarians 6.10% (1,227,623 inhabitants, Gypsies 3.09% (621,573 people, other 18 national minorities (Ukrainians, Germans, Turks, Russian-Lipovans, Tartars, Serbs, Slovaks, Bulgarians, Croats, Greek, Italians, Jews, Czechs, Poles, Chinese, Armenians, Csangos, Macedonians and the category “other national groups” have together only 1.21% (242,767 inhabitants. There is also a category of people for which the information was ”unavailable”, totaling 6.15% (1,236,810 people of the Romanian population (fig. 1. As requested to assess the essential issues of such a subject, one needs to analyze the distribution of the national minorities across Romania, at the level of the counties and the eight geographical-historical provinces of Romania: Transylvania, Moldavia, Dobrudja, Muntenia, Oltenia, Banat, Crişana and Maramureş. Generally, regarding the presence of the Romanian population, one notices the following situation at the level of the counties: in 18 counties of the total of 41 in Romania, the weight of the Romanian population is over 90%, including all the eight Moldavian counties (Bacău, Botoşani, Galaţi, Iaşi, Neamţ, Suceava, Vaslui, Vrancea, six out of the ten counties of Muntenia (Argeş, Brăila, Buzău, Dâmboviţa, Prahova, Teleorman and four out of five counties of Oltenia (Dolj, Gorj, Olt, Vâlcea. The highest weight of Romanians is recorded in the counties of Gorj (94.17% and Botoşani (94.08%. A weight between 80 and 90% was recorded in five out of the ten counties of

  4. Depletion of florfenicol amine in tilapia (Oreochromis sp.) maintained in a recirculating aquaculture system following Aquaflor®-medicated feed therapy

    Science.gov (United States)

    Gaikowski, Mark P.; Whitsel, Melissa K.; Charles, Shawn; Schleis, Susan M.; Crouch, Louis S.; Endris, Richard G.

    2015-01-01

    Aquaflor® [50% w w−1 florfenicol (FFC)], is approved for use in freshwater-reared warmwater finfish which include tilapia Oreochromis spp. in the United States to control mortality from Streptococcus iniae. The depletion of florfenicol amine (FFA), the marker residue of FFC, was evaluated after feeding FFC-medicated feed to deliver a nominal 20 mg FFC kg−1 BW d−1 dose (1.33× the label use of 15 mg FFC kg−1 BW d−1) to Nile tilapia O. niloticus and hybrid tilapia O. niloticus × O. aureus held in a recirculating aquaculture system (RAS) at production-scale holding densities. Florfenicol amine concentrations were determined in fillets taken from 10 fish before dosing and from 20 fish at nine time points after dosing (from 1 to 240 h post-dosing). Water samples were assayed for FFC before, during and after the dosing period. Parameters monitored included daily feed consumption and biofilter function (levels of ammonia, nitrite and nitrate). Mean fillet FFA concentration decreased from 13.77 μg g−1 at 1-h post dosing to 0.39 μg g−1 at 240-h post dosing. Water FFC concentration decreased from a maximum of 1400 ng mL−1 at 1 day post-dosing to 847 ng mL−1 at 240 h post-dosing. There were no adverse effects noted on fish, feed consumption or biofilter function associated with FFC-medicated feed administration to tilapia.

  5. Evaluation of thirteen lines (M8) of 'May flower' beans selected after gamma irradiation with cobalt 60

    International Nuclear Information System (INIS)

    For the nutrition of mexican people, cultivation of bean is necessary. The constant increase in population and the low yields in bean crops had bring as a consequence a great demand of beans, for which reason it is necessary to increase the production and productivity in order to satisfy such a demand. In 1986, in the state of Guanajuato the bean harvest was of 80, 119 hectare. In 1964 the harvest was 225458 hectare and began a lowering until reach in 1980 a surface of 64,122 hectare. The average yield estimated for 1960, was 309 kilogram-hectare, in 1975 the yield was 554 kilogram and starting from 1976 the trend was descendent until reach an average of 484 kilogram-hectare in 1980. According to estimations of Instituto Nacional de Investigaciones Agronomicas (INIA) in 1981, the diminishing in harvest surface and the low yields in crops were owing between other factors to: mosaic virus which is common in beans, roya, pest, etc. Utilization of radioinduced mutagenesis in feeding crops, constitute an alternative for the obtention of new genotypes more productive and resistant to diseases which can help to solve the great demand for this crop. The goal of this work is the obtention of mutants resistant to the main diseases in El Bajio zone (Mosaic virus common to bean and roya) in join with higher yields, starting from irradiation of the variety of bean 'May flower' with gamma radiation from cobalt 60. (Author). 6 refs, 2 tabs

  6. Characterization and expression analysis of a peptidoglycan recognition protein gene, SmPGRP2 in mucosal tissues of turbot (Scophthalmus maximus L.) following bacterial challenge.

    Science.gov (United States)

    Zhang, Linan; Gao, Chengbin; Liu, Fengqiao; Song, Lin; Su, Baofeng; Li, Chao

    2016-09-01

    Peptidoglycan recognition receptor proteins (PGRPs), a group of pattern recognition receptors (PRRs), can recognize peptidoglycan (PGN) of the bacteria cell wall and play an important role in host immune defense against pathogen infection. They are highly structurally conserved through evolution, but with different function in innate immunity between invertebrates and vertebrates. In teleost fish, several PGRPs have been characterized recently. They have both amidase activity and bactericidal activity and are involved in indirectly killing bacteria and regulating multiple signaling pathways. However, the knowledge of PGRPs in mucosal immunity of teleost fish is still limited. In this study, we identified a PGRPs gene (SmPGRP2) of turbot and investigated its expression patterns in mucosal tissues after challenge with Gram-positive bacteria Streptococcus iniae and Gram-negative bacteria Vibrio anguillarum. Phylogenetic analysis showed the strongest relationship of turbot PGRP to halibut, which was consistent with their phylogenetic relationships. In addition, SmPGRP2 was ubiquitously expressed in turbot tissues, and constitutive expression levels were higher in classical immune tissues (including liver, spleen, and head-kidney) than mucosal tissues (intestine, gill and skin). After bacterial challenge, the expression of SmPGRP2 was induced and showed a general trend of up-regulation in mucosal tissues, except in intestine following V. anguillarum infection. These different expression patterns varied depending on both pathogen and tissue type, suggesting its distinct roles in the host immune response to bacterial pathogen. PMID:27461422

  7. The mucosal expression signatures of g-type lysozyme in turbot (Scophthalmus maximus) following bacterial challenge.

    Science.gov (United States)

    Gao, Chengbin; Fu, Qiang; Zhou, Shun; Song, Lin; Ren, Yichao; Dong, Xiaoyu; Su, Baofeng; Li, Chao

    2016-07-01

    The mucosal surfaces constitute the first line of host defense against infection, and also serve as the dynamic interfaces that simultaneously mediate a diverse array of critical physiological processes, while in constantly contact with a wide range of pathogens. The lysozymes are considered as key components for innate immune response to pathogen infection with their strong antibacterial activities. But their activities in mucosal immune responses were always overlooked, especially for g-type lysozymes, whose expression patterns in mucosal tissues following bacterial challenge are still limited. Towards to this end, here, we characterized the g-type lysozymes, Lyg1 and Lyg2 in turbot, and determined their expression patterns in mucosal barriers following different bacterial infection. The phylogenetic analysis revealed the turbot g-type lysozyme genes showed the closest relationship to Cynoglossus semilaevis. The two lysozyme genes showed different expression patterns following challenge. Lyg2 was significantly up-regulated in mucosal tissues following Vibrio anguillarum and Streptococcus iniae challenge, while Lyg1 showed a general trend of down-regulation. The significant mucosal expression signatures of g-type lysozyme genes indicated their key roles to prevent pathogen attachment and entry in the first line of host defense system. Further functional studies should be carried out to better characterize the availability of utilization of g-type lysozyme to increase the disease resistance in the mucosal surfaces and facilitate the disease resistant breeding selection. PMID:27189917

  8. Abstracts of the 40th Symposium on Cuniculture, ASESCU Santiago de Compostela, Spain, 28th-29th May, 2015

    Directory of Open Access Journals (Sweden)

    Abstracts, Conferences, Congresses, Symposiums...

    2015-06-01

    Full Text Available The 40th Congress of the Spanish Association of Cuniculture (ASESCU was held in Santiago de Compostela from 28th to 29th May 2015. This is the 40th edition of the conference, held annually without interruption since 1976. Main papers focused on analysing the current rabbit sector situation in the north-western Iberian Peninsula, genetic improvement of meat rabbit and foundation of new lines. The future of meat rabbit production and the rabbit meat production value chain was discussed, with the results of the partnership between the Spanish Institute of Agricultural Research (INIA and the rabbit marketing board (INTERCUN to support research projects. A round table was also held on coccidiosis in rabbit farms. In addition, a total of 32 communications were presented both in working sessions with oral communications and posters (pathology, nutrition, reproduction, ethology, production and products. The meeting was attended by more than 200 participants, including researchers from Spain, Portugal, Mexico, Ecuador and Egypt, among other countries. Abstracts of the contributions presented are reported below.

  9. Commercial biopreservatives combined with salt and sugar to control Listeria monocytogenes during smoked salmon processing.

    Science.gov (United States)

    Montiel, Raquel; Bravo, Daniel; Medina, Margarita

    2013-08-01

    Three commercial antimicrobials, applied during the salting stage in the preparation of cold-smoked salmon, were investigated for their effect on the behavior of Listeria monocytogenes. Fresh salmon inoculated with L. monocytogenes INIA 2530 was treated with three bacteriocin-based commercial biopreservatives, which were applied in combination with a salt-sugar mix. The product was kept at 8°C for 7 days. L. monocytogenes grew by approximately 3 log CFU/g in control salmon (without the salt-sugar mix or biopreservatives). Pathogen levels were reduced by the three biopreservatives investigated. After 7 days at 8°C, L. monocytogenes counts in salmon treated with biopreservatives combined with the salt-sugar mix were significantly lower than those observed in salmon treated with only salt and sugar. At the end of storage, salmon treated with biopreservative derived from Pediococcus acidilactici had pathogen levels 3.6 log CFU/g lower than in control salmon (without the salt-sugar mix) and 1.5 log CFU/g lower than in the samples treated with only salt and sugar. The application of commercial biopreservatives to fresh salmon during the dry-salting stage might help control L. monocytogenes growth, thus enhancing the safety of cold-smoked salmon during refrigerated storage. PMID:23905807

  10. 高良姜与大高良姜精油中活性物质的比较

    Institute of Scientific and Technical Information of China (English)

    张倩芝; 蔡明招; 卢志毅

    2006-01-01

    姜科植物是分布于热带和亚热带药材中的重要家族,姜科分为2亚科、3族,约49属、1500种,包括姜黄Curcuma longa L.、小豆寇Elettaria cardamomum(Roxb.)Maton、生姜Zingiber officinale ROSC.、莪术Curcuma zedoaria(Berg.)Rosc.、大高良姜Alpinia galanga(L.)Willd.、益智A珍inia oxyphylla Miq.和山柰Kaempferia galanga Linn.等。姜科植物主产地为亚洲热带,我国有19属,150余种,分布于东南至西南部各省区,是东南亚各国及我国人民日常生活中常用的植物。

  11. MORPHOPHYSIOLOGICAL CHANGES OF IPOMOEA BATATAS (L. LAM. DURING DROUGHT STRESS

    Directory of Open Access Journals (Sweden)

    La Rosa, R.

    2008-01-01

    Full Text Available One of the greatest problems in the world is desertification so we must to know physiological responses and morphological adaptations from species with tolerance to drought stress. Sweet potato (Ipomoea batatas Lam. is one of the most important crops of the world, especially because of its great tolerance to drought stress. Hence, we decided to know what morphophysiological and metabolic responses has I. batatas under drought stress. So, we got cuttings of variety INA-100, from INIA Huaral, and were planted in 22 recipients of 4 L with substrate consisting in a mixing of sand and humus (3:1. Half of them were irrigated monthly and other half only receives water to establish plants and no more after that. We measured transpiration, histological changes in leaves and metabolic changes at protein and starch level. We confirmed transpiration is not affected for air humidity or substrate humidity. But, the effect of drought can be better observed in foliage growth, therefore in histological changes in leaves. No significance in concentration of proteins but significance in carbohydrates concentration shows an osmotic regulation in order to maintain stomata open and also an increase in photosynthetic activity in stressed plants.

  12. BIOFILMS BASED ON CANIHUA FLOUR (Chenopodium Pallidicaule: DESIGN AND CHARACTERIZATION

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    Lady M. Salas-Valero

    2015-01-01

    Full Text Available This work aims to (1 produce and characterize the flour obtained from two varieties of canihua, cupi and illpa-inia, and (2 evaluate the ability of these flours to form biofilms. The flours produced contain proteins, starches, lipids, organic substances containing phenol groups, and high percentages of unsaturated fatty acids. Films produced from the illpa variety presented lower water vapor permeability and larger Young’s modulus values than the films formed from the cupi variety. Both films were yellowish and displayed a high light blocking ability (as compared with polyethylene films, which can be attributed to the presence of phenolic compounds. Furthermore, they showed lesser solubility and water permeability than other polysaccharide films, which may be the result of the higher protein (12%–13.8% and lipid (11% contents in canihua flours, as well as the formation of a larger number of S–S bonds. On the other hand, these films presented a single vitreous transition temperature at low temperatures (< 0 °C, crystallization of the A and Vh types, and an additional diffraction peak at 2 = 7.5º, ascribed to the presence of essential fatty acids in canihua flour. Canihua flour can form films with adequate properties and shows promise for potential applications in food packaging, because it acts as a good barrier to incident ultraviolet light.

  13. Evaluación del rendimiento de grano seco en accesiones promisorias de Plukenetia volubilis “sacha inchi” en Loreto

    Directory of Open Access Journals (Sweden)

    Andrés Fernández-Sandoval

    2013-06-01

    Full Text Available El sacha inchi es una especie vegetal oleaginosa que se encuentra al estado silvestre en selva alta, baja y ceja de selva del Perú. Es importante por su alto contenido de ácidos grasos insaturados (aceites omegas y proteínas que contienen las semillas que lo hace ideal para mejorar la dieta alimenticia humana. El Gobierno Regional ha priorizado el cultivo de sacha inchi para impulsar el desarrollo económico y social de sus productores. Los trabajos de evaluación de rendimiento de grano seco de sacha inchi bajo condiciones de selva baja, se realizaron en el Campo Experimental El Dorado de la EEA. San Roque- INIA; con 9 accesiones provenientes del banco de germoplasma de la EEA. El Porvenir, Tarapoto. El objetivo de esta investigación fue evaluar el rendimiento de grano seco y validar y adaptar la tecnología de producción de esta especie en condiciones de selva baja. Las accesiones que sobresalieron fueron Barranquita y Cumbaza, con rendimientos de 1863 y 1809 kg/ha de grano seco por hectárea. La accesión Tambo Yaguas, obtuvo el rendimiento más bajo con 631 kg/ha de grano seco por hectárea, debido principalmente a su susceptibilidad a Rhizoctonia sp. “Mustia hilachoza”.

  14. Isolation and molecular identification of the etiological agents of streptococcosis in Nile tilapia (Oreochromis niloticus) cultured in net cages in Lake Sentani, Papua, Indonesia.

    Science.gov (United States)

    Anshary, Hilal; Kurniawan, Rio A; Sriwulan, Sriwulan; Ramli, Ramli; Baxa, Dolores V

    2014-01-01

    Infections with Streptococcus spp. were observed in Nile tilapia cultured in net cages in Lake Sentani, Papua, Indonesia. Clinical signs included exophthalmia, erratic swimming, ascites in abdominal cavity, and external hemorrhages. Four types of bacterial colonies (SK, K10, P20, and M12) were isolated from the brain, kidney, and eyes. Based on phenotypic and genetic (16S rDNA sequencing) characteristics, the isolates were identified as Streptococcus iniae (SK), Streptococcus agalactiae (K10 and P20) and Lactococcus garvieae (M12). The latter species has not been previously isolated or reported from fish streptococcosis in Indonesia. Intraperitoneal injection of healthy tilapia with the bacterial species caused significant morbidity (70%) within 3 days and 100% mortality at 6 days post injection. Experimental infections and reisolation of the bacteria from morbid and dead fish suggest they are the causative agents of streptococcosis, which rendered high mortality among cage cultured Nile tilapia in Lake Sentani. Our results suggest the need for developing diagnostic tools for accurate identification of the agents of streptococcosis. As tilapia aquaculture continues to expand as a means of food production and livelihood in Indonesia, it becomes crucial to ensure that fish resources are monitored and protected from the adverse effects of infectious diseases. PMID:25392797

  15. Relationship between the nutrition status and sensory characteristics of melon fertilized with wine-distillery waste compost

    Science.gov (United States)

    Requejo, María Isabel; Sánchez-Palomo, Eva; González, Miguel Angel; Castellanos, Maria Teresa; Villena, Raquel; Cartagena, Maria Carmen; Ribas, Francisco

    2015-04-01

    The interest in developing sustainable agriculture is becoming more important day by day. A large quantity of wastes from the wine and distillery industry are produced and constitute a serious problem not only environmental but also economic. The use of exhausted grape marc compost as organic amendment is a management option of the fertility of soils. On the other hand, consumers are increasingly concerned about the type, quality and origin of food production. Flavor and aroma are most often the true indicators of shelf-life from the consumer's point of view. The aim of this study was to relate the nutritional status of melon fertilized with exhausted grape marc compost with the sensory profile of fresh-cut fruits. A field experiment was established with three doses of compost (1, 2 and 3 kg per linear meter) and a control. Melons were harvested at maturity and the sensory evaluation was carried out by an expert panel of melon tasters to describe odour, flavour and texture. Nitrogen, phosphorus and potassium concentration was determined in the fruits to calculate nutrient absorption. Acknowledgements: This project has been supported by INIA-RTA2010-00110-C03-01

  16. Assessment of different doses of N applied to a melon crop in the quality of groundwater: environmental impact indexes.

    Science.gov (United States)

    Castellanos, M. T.; Cartagena, M. C.; Ribas, F.; Cabello, M. J.; Arce, A.; Tarquis, A. M.

    2010-05-01

    Provided that the intensive use of N in agricultural systems has negatively impacted environmental quality, because a higher fertilization requirements of the crop to make the nitrate leached into the aquifer contaminating, as well as increasing their presence on the ground resulting in a time of intercropping nitrates washing with the arrival of rains. It's necessary to know the N management to reduce the pollution risks and may lead the higher production with the fewer economic cost, environmental and health. The aim of this study was to asses how different doses of nitrogen applied to a melon crop affected the quality of groundwater. The doses of N applied ranged between 11 and 393 kg ha-1. Several indexes, based in N leaching consequence of the agricultural practice, were calculated and provided an essential tool for knowing the risks of groundwater pollution with the practices used. Drinking water impact, irrigation water management impact and environmental impact indexes were obtained during four years in a vulnerable area of the centre of Spain. Acknowledgements: This project has been supported by INIA-RTA04-111

  17. Distribution of marine birnavirus in cultured olive flounder Paralichthys olivaceus in Korea.

    Science.gov (United States)

    Jung, Sung-Ju; Kim, Seok-Ryel; Joung, Il-Yong; Kitamura, Shin-Ichi; Ceong, Hee-Taek; Oh, Myung-Joo

    2008-06-01

    Surveys of marine birnavirus (MABV) were undertaken in cultured olive flounder Paralichthys olivaceus from the south and west coastal areas and Jeju in Korea during the period January 1999 to April 2007. MABV was detected in all seasons from the fry, juveniles and adult fish from the areas examined. Evident cytopathic effects of the virus including rounding and cell lysis were observed in chinook salmon embryo (CHSE-214) and rainbow trout gonad (RTG-2) cells, but not in fathead minnow (FHM) and epithelial papilloma of carp (EPC) cells. Nucleotide sequences of the VP2/NS junction region of the Korean isolates showed 97.8% ~ 100% similarity, and they belonged to the same genogroup. Cross neutralization tests with serotype-specific rabbit antisera against MABV strains exhibited a close antigenic relationships between strains, and were distinct from infectious pancreatic necrosis virus (IPNV) strains. Coinfection of MABV with bacteria (Streptococcus iniae, Vibrio spp.) and viruses (nervous necrosis virus, lymphocystis disease virus, viral hemorrhagic septicemia virus) was observed. PMID:18604495

  18. Caracterización bioquímica de hojas de clones de Theobroma cacao y su relación con los tricomas

    Directory of Open Access Journals (Sweden)

    Janet Quiñones Galvez

    2015-11-01

    Full Text Available Theobroma cacao L. is a crop of major importance for their potential applications in pharmacy and agriculture. It contains metabolites that are of great interest for its natural biological functions in plant response to different types of stress, both biotic and abiotic. The presence, type and concentration of both primary and secondary metabolites may vary depending on the genotype analyzed and these compounds can accumulate in the architecture associated structures such as leaf trichomes. The aim of this study was to characterize 26 clones of the germplasm bank of Padrón, INIA-Miranda, Miranda State, Venezuela and study the relationship between biochemical indicators assessed and the number of trichomes. The content of phenolic compounds, soluble protein, free proline, total carbohydrates, lignins, the number of trichomes and the relationship between all traits was determined. The results showed that in the two dimensional space explained 46.68% of the variability, five homogeneous groups were defined: 10 clones were associated with the maximum gradient of proline, total carbohydrate and lignin content. Nine clones were associated with high content of phenols and a group of seven clones with the highest number of trichomes on leaves. It was found that the number of trichomes was not related to the concentration of phenolic compounds. The clones of Forastero cocoa type showed higher concentration of total phenols and carbohydrates, meanwhile the Trinitario type presented higher concentration of proteins and prolina.

  19. Culinary and sensory traits diversity in the Spanish Core Collection of common beans (Phaseolus vulgaris L.

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    Pedro A. Casquero

    2016-03-01

    Full Text Available The Spanish National Plant Genetic Resource Center’s core collection of bean germplasm includes 202 accessions selected from more than 3000 accessions in function of passport data, seed phenotype, genetic background, and agronomic traits. To acquire more useful information about these accessions, we cultivated and characterized them for sensory and culinary traits. We found considerable variation for culinary and sensory traits of the cooked beans (mean coefficients of variation: 41% for the sensory traits and 40% for the culinary traits. The large dataset enabled us to study correlations between sensory and culinary traits and among these traits and geographic origin, seed color, and growth habit. Greater proportion of white in the seed coat correlated positively with brightness and negatively with mealiness (r=0.60, r=-0.60, p<0.001, respectively. Mealiness correlated negatively with seed-coat roughness and rate of water absorption (r=-0.60, r=-0.53, p<0.001, respectively. Materials of Andean origin had lower seed-coat brightness (p<0.01 and seed-coat roughness, and greater seed-coat perceptibility, mealiness, flavor, and aroma (p<0.001 than materials of Mesoamerican origin. Growth habit failed to correlate with culinary or sensory traits. Breeders can benefit from the information about this core collection available at www.crf.inia.es/crfesp/paginaprincipaljudia.asp.

  20. Correlation and heritability Analysis in the genetic improvement of camu-camu

    Directory of Open Access Journals (Sweden)

    Mario Pinedo Panduro

    2012-03-01

    Full Text Available In Peru and Brazil have been made between 2002 and 2011, correlation and heritability in search of tools for genetic improvement of camu-camu. We studied basic collections, comparative and progeny clones exist in the INIA, IIAP and INPA. The length of petiole (LP, has a half heritability (in the broad sense of h2 g = 0.42 and correlation coefficients of r2 = 0.37 with fruit yield and r2 = 0.54 with fruit weight. Basal branch number (NRB also shows levels of heritability average (in the strict sense: h2 a = 0.45 and h2 g = 0.33 in the broad sense. NRB in turn significantly correlated with fruit yield (RF (r2 = 0.43, fruit weight (FW (r2 = 0.38 and ascorbic acid (AA (r2 =- 0.30. The values of pH and soluble solids (degrees Brix of the pulp showed a high correlation with AA (r2 = 0.85 and r2 = 0.94 respectively. In light of the information correlation and heritability, we emphasize that the parameters "number of basal branches", "petiole length" and "fruit weight" and present a relatively high correlation with "yield fruit" also have a level intermediate heritability, which qualify them as important tools for the selection of superior plants camu-camu

  1. Characterization of native strains of Azospirillum spp. and its effect on growth of Oryza sativa L. “rice” in Lambayeque

    Directory of Open Access Journals (Sweden)

    Franklin García

    2010-06-01

    Full Text Available This research was conducted to characterize and determine the effect of native strains of Azospirillum spp. on growth of Oryza sativa L. "rice" as an alternative for the reduction of chemical fertilizers. The bacteria were isolated from roots of rice in Lambayeque. Fragments of previously disinfected roots were planted in Nfb semisolid medium, were nitrogen-fixing bacteria were recognized by a whitish film on the surface and turn from green to blue. The genus Azospirillum was identified in Congo red agar medium, obtained 96 isolates with production of 2.69 to 38.02 ppm indoleacetic acid and 7.95 to 29.09 ppm of fixed nitrogen. The four strains with the highest values were inoculated independently and in combination with 50 and 100 % urea in Tinajones INIA508 rice. It used a completely randomized design and including witnesses’ 50 and 100 % urea and absolute control. All native strains increased height and dry matter of shoot and roots, with effectiveness index of up to 21.77, 102.06 and 126.11 % compared to untreated control. Azospirillum sp. GM-86 selected by the highest value of indoleacetic acid, reached the highest level of effectiveness in height and root dry biomass. The application of 50 % úrea with Azospirillum spp. increased rates of effectiveness. It demonstrated the potential of native strains of Azospirillum spp. to increase the growth of rice as well as the possibility to use associate with low doses of urea.

  2. Bacterial zoonoses of fishes: a review and appraisal of evidence for linkages between fish and human infections.

    Science.gov (United States)

    Gauthier, David T

    2015-01-01

    Human contact with and consumption of fishes presents hazards from a range of bacterial zoonotic infections. Whereas many bacterial pathogens have been presented as fish-borne zoonoses on the basis of epidemiological and phenotypic evidence, genetic identity between fish and human isolates is not frequently examined or does not provide support for transmission between these hosts. In order to accurately assess the zoonotic risk from exposure to fishes in the context of aquaculture, wild fisheries and ornamental aquaria, it is important to critically examine evidence of linkages between bacteria infecting fishes and humans. This article reviews bacteria typically presented as fish-borne zoonoses, and examines the current strength of evidence for this classification. Of bacteria generally described as fish-borne zoonoses, only Mycobacterium spp., Streptococcus iniae, Clostridium botulinum, and Vibrio vulnificus appear to be well-supported as zoonoses in the strict sense. Erysipelothrix rhusiopathiae, while transmissible from fishes to humans, does not cause disease in fishes and is therefore excluded from the list. Some epidemiological and/or molecular linkages have been made between other bacteria infecting both fishes and humans, but more work is needed to elucidate routes of transmission and the identity of these pathogens in their respective hosts at the genomic level. PMID:25466575

  3. Antimicrobial activity of reuterin produced by Lactobacillus reuteri on Listeria monocytogenes in cold-smoked salmon.

    Science.gov (United States)

    Montiel, R; Martín-Cabrejas, I; Langa, S; El Aouad, N; Arqués, J L; Reyes, F; Medina, M

    2014-12-01

    Lactobacillus reuteri INIA P579 was used for the production and purification of reuterin. The purity of reuterin was assessed by high resolution electrospray ionization mass spectrometry (HRESIMS) and nuclear magnetic resonance (NMR) spectroscopy. After purification, reuterin concentration obtained was 1.3 M. The inhibitory activity using Escherichia coli K12 as indicator strain was estimated to be 510 AU/ml. Survival curves in tryptic soy broth revealed that reuterin required to inhibit the growth of three Listeria monocytogenes strains was in the range of 2-4 AU/ml. Purified reuterin (10 AU/g) significantly reduced the growth of L. monocytogenes in cold-smoked salmon kept under moderate or strong temperature abuse conditions. After 15 d at 8 °C, cold-smoked salmon with added reuterin exhibited L. monocytogenes counts 2.0 log CFU/g lower than control smoked salmon with no reuterin added. At 30 °C, reuterin also controlled the growth of the pathogen, with counts 1.4 and 0.9 log CFU/g lower than those observed in control smoked salmon after 24 and 48 h, respectively. The addition of purified reuterin might be used as a hurdle technology to improve the safety and extend the shelf-life of lightly preserved seafood products such as cold-smoked salmon.

  4. Dietary Supplementation with Allspice Pimenta dioica Reduces the Occurrence of Streptococcal Disease during First Feeding of Mozambique Tilapia Fry.

    Science.gov (United States)

    Yılmaz, Sevdan; Ergün, Sebahattin

    2014-09-01

    Abstract Allspice Pimenta dioica as a feed additive was studied for its effects on growth performance and disease resistance in Mozambique Tilapia Oreochromis mossambicus. Five isonitrogenous (36% crude protein) and isocaloric (18.5 kJ/g) diets were formulated to contain 0 (control), 5, 10, 15, or 20 g of allspice/kg of fish feed. In a 50-d feeding trial, 15 plastic tanks (21 L) were stocked with 35 fish fry (0.012 g) each. After the feeding trial, fish were exposed to Streptococcus iniae and mortalities were recorded. The second-order polynomial regression indicated that the dietary allspice level of 10 g/kg provided the best growth performance and feed utilization. The greatest survival after pathogen challenge was also obtained from the diet supplemented with allspice at 10 g/kg. Therefore, allspice acts as a growth promoter to improve feed utilization and weight gain in Mozambique Tilapia fry and acts an antimicrobial agent to enhance disease resistance during first feeding of fry. These results suggest that allspice can be used as an alternative to antibiotics in controlling streptococcal disease in tilapia culture. Received October 19, 2012; accepted January 20, 2014. PMID:25229484

  5. Anaerobic green fluorescent protein as a marker of Bifidobacterium strains.

    Science.gov (United States)

    Landete, José M; Peirotén, Ángela; Rodríguez, Eva; Margolles, Abelardo; Medina, Margarita; Arqués, Juan L

    2014-04-01

    Some strains of Bifidobacterium are considered as probiotics and are being added as adjunct culture in food products due to their potential in maintaining a healthy intestinal microbial balance. However, despite these benefits, bifidobacteria still remain poorly understood at the genetic level compared with other microorganisms of industrial interest. In this work, we have developed a non-invasive green fluorescent based reporter system for real-time tracking of Bifidobacterium species in vivo. The reporter vector pNZ:Tu-GFPana is based on the pNZ8048 plasmid harboring a bifidobacterial promoter (elongation factor Tu from Bifidobacterium longum CECT 4551) and a fluorescent protein containing a flavin-mono-nucleotide-based cofactor (evoglow-Pp1) which is fluorescent under both aerobic and anaerobic conditions. pNZ:Tu-GFPana was constructed and found to stably replicate in B. longum CECT 4551 and in the intestinal strain Bifidobacterium breve INIA P734. The subsequent analysis of these strains allowed us to assess the functionality of this plasmid. Our results demonstrate the potential of pNZ:Tu-GFPana as a real-time reporter system for Bifidobacterium in order to track the behavior of this probiotic species in complex environments like food or intestinal microbiota, and to estimate their competition and colonization potential. PMID:24495586

  6. Anomalias ósseas congênitas em Sotalia guianensis (Mammalia, Cetacea, Delphinidae da costa centro-norte do Estado do Rio de Janeiro Congenital bone defects in Guiana dolphin, Sotalia guianensis (Mammalia, Cetacea, Delphinidae in the northcentral coast of Rio de Janeiro State, Brazil

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    Maíra Laeta

    2010-06-01

    Full Text Available Carcaças de botos-cinza Sotalia guianensis (van Bénéden, 1864 foram recuperadas entre 20 de agosto de 2001 e 13 de fevereiro de 2006, na costa centro-norte fluminense. A maior parte delas (85% entre Barra de São João (22º35'S 41º59'W e Quissamã (22º06'S 41º28'W. Foram estudadas as colunas vertebrais dos 20 exemplares que apresentavam mais de 60% das vértebras presentes. A coleção é formada por 75% de animais imaturos, e todos os esqueletos mostram alterações tafonômicas mínimas. Duas categorias de anomalias congênitas foram diagnosticadas, ambas relacionadas ao desenvolvimento do mesoderma paraxial. A sétima vértebra cervical (C7 foi a única afetada, as costelas cervicais e o não fechamento do arco neural atingiram 15 (75% dos indivíduos analisados, dos quais três (15% apresentam ambas as anomalias. Nove (45% indivíduos apresentaram costelas cervicais uni ou bilateral, e nove (45% indivíduos apresentaram não fechamento do arco neural; em todos os casos as vértebras contíguas eram normais. A ocorrência de anomalias nesta série do Rio de Janeiro é maior do que as referidas na literatura brasileira para outras séries de Sotalia do Amazonas, Ceará e Santa Catarina. A série de Sotalia descrita vem de uma região do litoral muito limitada e provavelmente representa uma população local. As costelas cervicais são geneticamente determinadas e podem estar concentradas por uma condição de grande proximidade biológica entre os animais; o não fechamento do arco pode ter também um componente ambiental, a ser investigado futuramente.Carcasses of Guiana dolphins, Sotalia guianensis (van Bénéden, 1864 were recovered from August 20, 2001 to February 13, 2006 along the north-central coast of Rio de Janeiro state. Most of the animals (85% were found between Barra de São João (22º35'S 41º59'W and Quissamã (22º06'S 41º28'W. We studied the vertebral columns of all the 20 specimens that had more than 60% of the

  7. La structure de la population après son lieu de naissance

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    Maria-Magdalena LUPCHIAN

    2008-10-01

    Full Text Available La capacité de polarisation de la ville de Suceava a agrandi d`une manière significative pendant la deuxième moitie de la XXème siècle, grâce au développement de la fonction industrielle de la ville entre 1960 et 1970. L`analyse de la structure de la population après le lieu de naissance a été possible grâceaux données fournies par les recensements de la population de 1930, 1966, 1977, 1992 et 2002(pour toute la ville et grâce aux données fournies par l`enquête réalisée en 2005 sur enéchantillon qui a dépassé 3000 personnes (pour l`analyse au niveau intra urbaine.Dans une première étape, l`aire de provenance de la population de la ville dépassaitles limites du département de Suceava. La ville a consolide son pouvoir d`attraction au niveaudu département et au niveau du Plateau de Suceava après les années `70. Pendant ce période(1950 – 1990, des déplacements très stables se sont développé entre les départements deBotoşani et de Neamţ et le département de Suceava.L`analyse de l`aire de provenance de la population née dans le département deSuceava relève le fait que la ville a représenté et représente encore un pole d`attraction pour lamoitie de plateau du département de Suceava. La ville chef lieu de département est enconcurrence avec les villes plus petites mais viables (Rădăuţi, Fălticeni, situées en proximité.L`analyse de la structure de la population après son lieu de naissance au niveau intraurbaine démontre l`opposition qui existe entre les vieux quartiers d`habitat individuel (ou lapopulation née en ville est majoritaire et les nouveaux quartiers, d`habitat collectif, édifiésdans les années d`industrialisation de la ville – Burdujeni, George Enescu – où le pourcentagede ceux nes dans le milieu rural est maximal.On remarque aussi la position intermédiaire du quartier Centre et des quartierspéricentraux, mais aussi du nouveau quartier Obcine, avec des pourcentage sur la

  8. Adaptación al cambio climático y la variabilidad: algunas opciones de respuesta para la producción agrícola en Uruguay Adaptation to climatic change and variability: some response options to agricultural production in Uruguay

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    Agustín Giménez

    2012-06-01

    Full Text Available Adaptación al cambio climático y la variabilidad: "algunas opciones de respuesta para la producción agrícola en Uruguay". Como se señala en varios informes del Panel Intergubernamental sobre Cambio Climático (IPCC, 2007a; IPCC, 2007b, la comunidad científica internacional expresa que el aumento en la concentración de gases de efecto invernadero tiene como resultado cambios en la variabilidad climática diaria, estacional, interanual y a lo largo de decenios. La variabilidad climática y la ocurrencia de eventos extremos (heladas, granizos, sequías resulta en perjuicios muy importantes para el sector agropecuario y frecuentemente el sector requiere de varios años para recuperarse económica y financieramente de los daños ocasionados. La unidad de agroclima y sistemas de información (GRAS del Instituto Nacional de Investigación Agropecuaria (INIA de Uruguay en conjunto con el Banco Mundial (BM y la Universidad de Cornell (UC de los Estados Unidos de América, finalizaron en el año 2009 el proyecto "Vulnerabilidad al cambio climático en los sistemas de producción agrícola en América Latina y el Caribe: "desarrollo de respuestas y estrategias", (Giménez y Lanfranco, 2009. El objetivo de la propuesta fue formular un plan de acción con recomendaciones para el desarrollo de respuestas y estrategias con el fin de contribuir a una mejor adaptación a los impactos de la variabilidad climática y la ocurrencia de eventos climáticos extremos en los sistemas de producción agrícola de Uruguay. Como resultado del trabajo, se identificaron y priorizaron 3 opciones de respuesta: sistema de información y soporte para la toma de decisiones; gestión del agua; y seguros y otros instrumentos financieros para la gestión de riesgos.Adaptation to climatic change and variability: "some response options to agricultural production in Uruguay". Like is mentioned in several reports of Intergovernmental Panel about Climatic Change (IPCC, 2007a; IPCC

  9. Evaluation of the Impact of Climatic Change on the Economic Value of Land in Agricultural Systems in Chile Evaluación del Impacto del Cambio Climático sobre el Valor Económico de la Tierra en Sistemas Agrícolas de Chile

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    Jorge González U

    2008-03-01

    Full Text Available Climatic change will affect crop yields and management. By the year 2050, the mean temperature could increase by 1.5 ºC; and by the year 2100 between 1.0 to 3.5 ºC. There are few studies on this subject in Chile. At the international level, estimated climatic changes in temperate and tropical zones could negatively affect wheat (Triticum vulgare L. and corn (Zea mays L. production, as examples. The objective of this study was to determine the relationship between agricultural systems and climatic change by using the Ricardian Method. Specific objectives were to evaluate and quantify the relationship of climatic variables (precipitation and temperature with economic variables under several realities of farms, to simulate the impact of scenarios of climatic change, to propose general orientations of adaptation and to evaluate the Ricardian Method with Chilean data. Economic and productive information from farmers belonging to Technological Transfer Groups (GTT of the Agricultural Research Institute (INIA was collected. The Ricardian Method explained 37.6% of land value variation. The highest values were in areas with moderate temperatures and precipitation. Temperature had a lower relationship to land value than precipitation. Under specific conditions (type of producer, irrigation, extension were detected behaviors that require further analysis. Upon simulating change of temperature and precipitation, the negative impacts on land value tended to be of lower magnitude than in other warmer regions. A tendency was observed for increased temperature to be beneficial, and a neutral to positive effect with less precipitation. The outputs could initially guide specific strategies of adaptation and mitigationEl cambio climático afectará los rendimientos y manejo de los cultivos agrícolas. Se estima que al año 2050 la temperatura media aumentaría 1,5 ºC, y al 2100 entre 1,0 y 3,5 ºC. En Chile existen pocos estudios sobre el tema

  10. Preliminary behavioural study of Caballo Fino Chilote stallions with restricted access to space and water during summer Estudio preliminar del comportamiento de potros raza Caballo Fino Chilote con acceso restringido a espacio y agua durante el verano

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    TA Tadich

    2010-01-01

    Full Text Available The Caballo Fino Chilote (CFCh is the only Chilean pony breed and a unique genetic resource. A group of seven CFCh stallions were observed for 60 hours, during summer, at INIA- Butalcura research station, Chiloé Island, under restricted space and access to water conditions. Scan sampling every five minutes was used to register 5 behavioural states and continuous focal sampling for 19 behavioural events. Inter-male interactions had a low frequency of presentation being avoidance (1.2 average events per stallion per day the most common. Overall stallions spent 55.89% (± 4.3% of their time foraging, 2.5% (± 0.82% in locomotion, 7.26% (± 0.82% standing alert, 32.24% (± 2.7% resting and 1.86% (± 0.64 in other behaviours. Foraging was the main activity, its occurrence was significantly lower (P El Caballo Fino Chilote (CFCh es la única raza de pony chileno y un recurso genético único. Un grupo de 7 potros CFCh fue observado por 60 horas, durante el verano, en la estación experimental del INIA-Butalcura, Isla de Chiloé, bajo condiciones de restricción de espacio y agua. El método de muestreo por escaneo se utilizó cada 5 minutos para evaluar estados conductuales y focal continuo para los eventos. Se registraron 5 estados y 19 eventos conductuales. Las interacciones entre potros tuvieron una frecuencia de presentación baja, siendo la más común “evasión” con un promedio de 1,2 eventos por potro por día. Los equinos utilizaron un 55,89% (± 4,3% del tiempo en forrajear, 2,5% (± 0,82% en locomoción, 7,26% (± 0,82% de pie alerta, 32,24% (± 2,7% descansando y 1,86% (± 0,64 en otras actividades. Forrajeo fue la principal actividad, siendo significativamente más baja (P < 0,05 durante el mediodía, periodo donde el descanso fue significativamente mayor (P < 0,05 comparado con los periodos de mañana y tarde. Se encontró una correlación positiva significativa entre temperatura y descanso y una correlación negativa significativa

  11. Udder Morphological Traits and Milk Yield of Chilota and Suffolk Down Sheep Breeds Morfología Mamaria y Producción Lechera de las Razas Ovinas Chilota y Suffolk Down

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    María E Martínez

    2011-03-01

    Full Text Available Morphological traits of the whole udder (circumference, width, height, and length, teats (angle, length and width, udder cistern (teat opening and cistern height, and milk yield were measured over one lactation in a total of 18 ewes (nine Chilota and nine Suffolk Down sheep belonging to the same experimental flock (Centro Experimental INIA Butalcura, Chiloé, Chile. Analysis of variance showed significant effects for breed, sheep within breed, and stage of lactation. Chilota ewes evidenced greater udder circumference (433 mm, udder length (86 mm, teat opening (96º, and milk yield (947 mL d-1 than Suffolk Down ewes (410 mm, 77 mm, 93º, and 559 mL d-1, respectively. Udder circumference, udder width, udder length, cistern height and teat size significantly decreased throughout lactation. Repeatabilities of all traits were between 0.17 and 0.60; the highest repeatability was for udder circumference. This trait is easy to measure and had a discriminatory value for breed differentiation. In conclusion, Chilota sheep had a dairy aptitude greater than Suffolk Down sheep and similar to Spanish and Mediterranean dairy sheep breeds at beginning of their breeding programs.Los caracteres morfológicos de la ubre (perímetro, anchura, longitud y altura, de los pezones (ángulo de inclinación, longitud y anchura, de las cisternas mamarias (apertura de los pezones y altura de la cisterna y la producción lechera fueron medidos a lo largo de una lactación en un total de 18 ovejas del mismo rebaño (Centro Experimental INIA Butalcura, Chiloé, Chile, nueve de raza Chilota y nueve de raza Suffolk Down. El análisis de varianza mostró efectos significativos de la raza, la oveja dentro de la raza y del estado de lactación sobre las variables estudiadas. La raza Chilota evidenció un mayor perímetro de la ubre (433 mm, longitud de la ubre (86 mm, abertura de los pezones (96º, y producción lechera (947 mL d-1 que la Suffolk Down ( 410 mm, 77 mm, 93º, y 559 m

  12. Aplicação de modelos não-lineares para descrever a evolução de características de crescimento e carcaça em bovinos da raça Hereford Application of nonlinear models to describe the evolution of growth and carcass traits in Hereford cattle

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    Rafael Espigolan

    2013-03-01

    Full Text Available Este trabalho foi desenvolvido com o objetivo de estudar a evolução das características de crescimento e carcaça em função da idade em bovinos da raça Hereford, utilizando modelos não-lineares. Foram utilizados dados de 240 bovinos machos (castrados da raça Hereford, pertencentes à fazenda Experimental "La Estanzuela" do INIA, Colonia, Uruguai. As características começaram a ser mensuradas a partir da desmama de animais com aproximadamente seis meses de idade, até o abate (dois anos de idade. As medições de peso foram realizadas a cada 15±3 dias durante todo o período e sem jejum prévio. As mensurações de área de olho de lombo, obtidas por ultrassom, e altura dos animais foram obtidas a cada 90 dias, sendo que as medidas de ultrassom foram tomadas entre a 12ª e 13ª costelas. Os modelos não-lineares utilizados foram os modelos de Brody, Gompertz, Logístico e Von Bertalanffy. Para as características peso, altura e área de olho de lombo, todos os modelos avaliados atingiram a convergência. Para descrever a evolução dos dados de peso e área de olho de lombo em função da idade, o modelo de Von Bertalanffy foi o mais indicado, e os modelos de Brody e Logístico mostraram o pior ajuste, respectivamente. A evolução da altura dos animais desde o desmame até os dois anos de idade pode ser modelada através de qualquer um dos modelos não-lineares considerados neste estudo.The objective of this research was to study the evolution of growth and carcass traits as a function of age in Hereford cattle using nonlinear models. Records from 240 Hereford steers (castrated, belonging to the experimental station "La Estanzuela" of INIA Uruguay, were utilized. The animals were measured from weaning, approximately at 6 months of age, to slaughter (two years of age. The animals were weighed, without fasting, every 15±3 days throughout the period. The measurements of ribeye loin area (between the 12th and 13th rib were obtained by

  13. Adaptability and Genotype x Environment Interaction of Spring Wheat Cultivars in Chile using Regression Analysis, AMMI, and SRAG Adaptabilidad e Interacción Genotipo x Ambiente en Trigos de Primavera utilizando Análisis de Regresión, AMMI y SREG

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    Dalma Castillo

    2012-06-01

    Full Text Available Wheat (Triticum aestivum L. genetic improvement objectives include obtaining cultivars capable of expressing their maximum potential yield and quality in diverse environments. This make necessary to know and define the environment in which a variety can express its maximum potential yield and quality. The objective of this study was to assess which method is the most efficient to study cultivars response in multiple environments. For this, we analyze the adaptability, stability, and genotype x environment (GxE interaction effect, grain yield, sedimentation, and wet gluten content of 13 spring wheat cultivars sown in six environments in the central-south and southern zones of Chile during two seasons. The data were analyzed by regression analysis, additive main effects and multiplicative interaction (AMMI, and the sites regression (SREG model. By this was thus established that SREG analysis is the most efficient for this type of study since, in addition to analyzing stability, adaptability, and effect (GxE, it allows identifying the best cultivar. In this case, 'Pandora-INIA' stands out by exhibiting the best yield (7.38 t ha-1, high sedimentation (36.95 cm³, and wet gluten (41.54% indices in all the environments, and this positions it as a variety having both high yield and quality.Dentro de los objetivos del mejoramiento genético del trigo (Triticum aestivum L. figura la obtención de variedades capaces de expresar su máximo potencial de rendimiento y calidad en diversos ambientes. Por lo cual es necesario conocer y definir el ambiente en que una variedad puede expresar al máximo su potencial de rendimiento y de calidad. El objetivo de este trabajo fue determinar una metodología eficiente para analizar la respuesta de diversos cultivares de trigo a distintos ambientes. Para ello se analizó la adaptabilidad, estabilidad y la interacción Genotipo x Ambiente (GxE de 13 variedades de trigo de primavera, sembradas, en seis ambientes de la

  14. Causas de mortalidad de alpacas en tres principales centros de producción ubicados en puna seca y humeda del departamento de Puno (Causes of mortality of alpacas in three main centers of production located in dry and humid fist of the Puno department

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    Mamani Paredes, Javier

    2009-08-01

    Full Text Available ResumenEl presente estudio se efectuó en tres principales centros de producción alpaquera del departamento de Puno: Rural Alianza EPS, CIP “La Raya” – UNA Puno ubicados en la zona agro ecológica de Puna húmeda y Anexo Quimsachata de INIA – Puno ubicado en la zona agro ecológica de Puna seca; con el objetivo de determinar las principales causas de mortalidad de alpacas. Mediante un estudio observacional analítico de tipo retrospectivo, utilizando los registros de mortalidad de las campañas de producción de 1998 – 2000. Determinándose que las principales causas de mortalidad dealpacas son: Agentes infecciosos 51.70%, anormalidades orgánicas24.08%, causas accidentales 13.36%, causas nutricionales 7.83% yenfermedades parasitarias 3.03%. Las causas infecciosas de mayorfrecuencia fueron: Las neumonías 31.12%, entero toxemia 20.90%,estomatitis 17.46% y otras en menor frecuencia. La mortalidad por causas orgánicas son la Caquexia de los animales 58.88%, aunque en puna seca la torsión intestinal y atonía ruminal son de mayor frecuencia. Se encontró como causas accidentales de muerte a: Traumatismos 50.30%, depredadores 28.63%, falsa deglución 7.35%, asfixia 7.16% y otros con menor frecuencia. Las principales causas nutricionales de mortalidad son:Desnutrición 27.12%, inanición 23.73%, falta de leche 19.32% y otras en menor proporción. Las causas parasitarias de mortalidad se determino: Sarna 33.33%, sarcosistiosis 28.95%, coccidiosis 25.44%, gastroenteritis verminosa 10.52%, dictiocaulosis e hidatidosis 0.88% respectivamente.SummaryThe present study took place in three main centers of alpaquera production of the department of Puno: Rural Alliance EPS, CIP “the Ray” - a Fist located in the zone ecological land of humid Fist and Quimsachata Annex of INIA - Fist located in the ecological zone land of dry Fist; with the aim of determining the main causes of mortality of alpacas. By means of an analytical observacional study

  15. Caracterização isoenzimática de genótipos de arroz Isoenzymatic characterization of rice genotypes

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    Sandro Bonow

    2001-02-01

    Full Text Available Objetivou-se, neste trabalho, caracterizar isoenzimaticamente genótipos de arroz (Oryza sativa L.. A produtividade do arroz irrigado no Rio Grande do Sul é elevada, em virtude da alta tecnologia e sistema de irrigação usados, associados ao potencial alcançado pelas cultivares desenvolvidas através de melhoramento genético. Apenas seis ancestrais contribuem com 86% dos genes das cultivares mais plantadas. Como conseqüência desta estreita base genética, as cultivares apresentam um alto grau de parentesco e de similaridade de suas características morfológicas e agronômicas, o que dificulta a identificação varietal. A concorrência com genótipos, como arroz-vermelho e arroz-preto, de difícil controle por serem da mesma espécie que os cultivados, é considerada como um dos maiores problemas da cultura. Análises de isoenzimas podem ser usadas para o estudo da variabilidade e para estimar as relações genéticas existentes entre estes genótipos. Eletroforese em gel de poliacrilamida foi empregada, portanto, para caracterizar, através de isoenzimas de esterase, 6fosfogluconato desidrogenase, fosfoglucoisomerase e isocitrato desidrogenase em sementes e folhas de plântulas, e de fosfatase ácida e aspartato transaminase em folhas de plântulas, as cultivares BR-IRGA 409, BR-IRGA 410, BRS 6 ('Chuí', BRS 7 ('Taim', BRS Agrisul, INIA Taquari, El Paso L 144 e IRGA 417, e ecótipos de arroz-vermelho e arroz-preto. A análise de agrupamento, efetuada por meio do coeficiente de Jaccard e pelo método da média aritmética não ponderada (UPGMA, possibilitou a diferenciação de todos os genótipos, à exceção de BRS 6 ('Chuí' e BRS 7 ('Taim'. Três grupos foram identificados, incluindo-se, em um deles, os ecótipos de arroz-vermelho e arroz-preto, que apresentaram 95% de similaridade.The objective of this work was to characterize isoenzymaticly rice (Oryza sativa L. genotypes. Rice yields obtained in Rio Grande do Sul, Brazil, are high

  16. Effect of N-acetyl cysteine and glycine supplementation on growth performance, glutathione synthesis, anti-oxidative and immune ability of Nile tilapia, Oreochromis niloticus.

    Science.gov (United States)

    Xie, Shiwei; Zhou, Weiwen; Tian, Lixia; Niu, Jin; Liu, Yongjian

    2016-08-01

    An 8-week feeding trial was conducted to evaluate the effect of N-acetyl cysteine (NAC) and glycine supplementation on growth performance, glutathione (GSH) synthesis, anti-oxidative and immune ability of Nile tilapia, Oreochromis niloticus. Four practical diets were formulated, control, control +0.2% NAC, control +0.5% glycine, control +0.2% NAC +0.5% glycine. Each diet was randomly assigned to quadruplicate groups of 30 fish (approximately 9.5 g). The weight gain and specific growth rate were significantly increased with the supplementation of NAC and glycine. While they had no effect on feed efficiency feed intake and survival. Glutathion peroxidase (GPx) was increased by NAC and γ-glutamine cysteine synthase (γ-GCS) in plasma were increased by glycine. After the feeding trail, fish were challenged by Streptococcus iniae, fish fed the diet supplemented with NAC obtained significantly higher survival rate after 72 h challenge test. NAC also decreased malonaldehyde (MDA) in liver, increased glutathione S-transferase (GST) activity in plasma, up-regulated mRNA expression of Superoxide dismutase (SOD) and GPx in liver and headkidney. Dietary supplementation of glycine increased the anti-oxidative ability of tilapia through increase anti-oxidative enzyme activity (SOD, glutathione reductase, myeloperoxidase) and up-regulate anti-oxidative gene expression (SOD). Immune ability only enhanced by the supplementation of NAC through increased interleukin-1β (IL-1β) mRNA expression. These results clearly indicated that the supplementation of NAC and glycine can significantly improve the growth performance of tilapia, and NAC also enhance the anti-oxidative and immune capacity of tilapia, glycine could only enhance the anti-oxidative ability.

  17. CLONACIÓN Y FILOGENIA MOLECULAR DE UN SEGMENTO DEL GEN CODANTE DE LA ACTINA DE MYRCIARIA DUBIA “CAMU-CAMU”: UN CANDIDATO PARA GEN DE REFERENCIA

    Directory of Open Access Journals (Sweden)

    Juan Castro Gómez

    2012-12-01

    Full Text Available Myrciaria dubia “camu-camu” es un frutal amazónico caracterizado por su amplia variación de vitamina C. Pero los estudios genético moleculares que puedan explicar esta variación son limitados. Por ello nuestro objetivo fue realizar la clonación y filogenia molecular de un segmento del gen codante de la actina de M. dubia. Las muestras fueron obtenidas de la colección de germoplasma del INIA. Luego, el ARN fue purificado y mediante RT-PCR con cebadores degenerados se amplificó un segmento del gen. En base a la secuencia obtenida se diseñaron cebadores específicos para PCR en tiempo real. Los resultados muestran que se ha aislado, clonado y secuenciado un segmento del gen codante de actina de M. dubia y detectado su expresión en hojas, pulpa y cáscara de M. dubia. Así, con el soporte de herramientas bioinformáticas y uso de técnicas de biología molecular hemos aislado, clonado y secuenciado un segmento del gen codante de la actina de M. dubia. Asimismo, los análisis realizados muestran que el gen se expresa y presenta niveles similares de expresión en hojas, pulpa y cáscara de M. dubia. Sin embargo, es necesario realizar más experimentos a fin de verificar su estabilidad de expresión.

  18. Molecular cloning and characterization of c-type lysozyme gene in orange-spotted grouper, Epinephelus coioides.

    Science.gov (United States)

    Wei, Shina; Huang, Youhua; Cai, Jia; Huang, Xiaohong; Fu, Jing; Qin, Qiwei

    2012-08-01

    Lysozymes are key proteins of the host innate immune system against pathogen infection. In this study, a c-type lysozyme gene (Ec-lysC) was cloned and characterized from orange-spotted grouper, Epinephelus coioides. The full-length Ec-lysC cDNA is composed of 533 bp and encodes a polypeptide of 144-residue protein with 94% identity to lysC of Kelp grouper, Epinephelus bruneus. The genomic DNA of Ec-lysC consists of 4 exons and 3 introns, with a total length of 1897 bp. Amino acid sequence alignment showed that Ec-lysC possessed conserved catalytic residues (Glu50 and Asp67) and "GSTDYGIFQINS" motif. RT-PCR results showed that Ec-lysC transcript was most abundant in head kidney and less in muscle. The expression of Ec-lysC was differentially up-regulated in head kidney after stimulation with lipopolysaccharide (LPS), Vibrio alginolyticus and Singapore grouper iridovirus (SGIV). Subcellular localization analysis revealed that Ec-lysC was distributed predominantly in the cytoplasm. The recombinant Ec-lysC (rEc-lysC) had lytic activities against Gram-positive bacteria Micrococcus lysodeikticus, Staphylococcus aureus, Streptococcus iniae and Gram-negative bacteria V. alginolyticus. The lysozyme acted on M. lysodeikticus cell walls as shown by scanning electron microscopy (SEM). Furthermore, overexpression of Ec-lysC in grouper cells delayed the occurrence of CPE induced by SGIV and inhibited the viral gene transcription significantly. Taken together, Ec-lysC might play an important role in grouper innate immune responses to invasion of bacterial and viral pathogens. C-type lysozyme gene from E. coioides (Ec-lysC) was identified and characterized.

  19. Complement factor D homolog involved in the alternative complement pathway of rock bream (Oplegnathus fasciatus): Molecular and functional characterization and immune responsive mRNA expression analysis.

    Science.gov (United States)

    Godahewa, G I; Perera, N C N; Bathige, S D N K; Nam, Bo-Hye; Noh, Jae Koo; Lee, Jehee

    2016-08-01

    The complement system serves conventional role in the innate defense against common invading pathogens. Complement factor D (CfD) is vital to alternative complement pathway activation in cleaving complement factor B. This catalytic reaction forms the alternative C3 convertase that is crucial for complement-mediated pathogenesis. In this study, rock bream (Oplegnathus fasciatus) CfD (OfCfD) was characterized and OfCfD mRNA expression was investigated. OfCfD encodes 277 amino acids (aa) for a 30-kDa polypeptide. A domain analysis of the deduced OfCfD aa sequence showed a single serine protease trypsin superfamily domain, a serine active region, three active sites, and three substrate-binding sites. Pairwise sequence comparisons indicated that OfCfD has the highest identity (84.5%) with Oreochromis niloticus CfD. The phylogenetic tree revealed a common ancestral origin of CfD members, with fish CfD distinct from other vertebrate orthologs. The structural arrangement of the OfCfD gene (2451 bp) contained five exons interrupted by four introns. A spatial transcriptional analysis indicated that OfCfD transcripts constitutively expressed in all of the examined rock bream tissues, and that they were highest in the spleen and liver. In addition, OfCfD transcripts were immunologically upregulated by lipopolysaccharide (LPS) (12 h p.i.), Streptococcus iniae (12 h p.i.), rock bream iridovirus (RBIV) (6-12 h p.i.), and poly I:C (6 h p.i.) in spleen tissue. OfCfD is a trypsin protease and its recombinant protein showed strong protease activity similar to that of trypsin, indicating its catalytic function in the alternative pathway. Together, our findings suggest that OfCfD might be involved in immune responses in rock bream. PMID:27311435

  20. Effect of N-acetyl cysteine and glycine supplementation on growth performance, glutathione synthesis, anti-oxidative and immune ability of Nile tilapia, Oreochromis niloticus.

    Science.gov (United States)

    Xie, Shiwei; Zhou, Weiwen; Tian, Lixia; Niu, Jin; Liu, Yongjian

    2016-08-01

    An 8-week feeding trial was conducted to evaluate the effect of N-acetyl cysteine (NAC) and glycine supplementation on growth performance, glutathione (GSH) synthesis, anti-oxidative and immune ability of Nile tilapia, Oreochromis niloticus. Four practical diets were formulated, control, control +0.2% NAC, control +0.5% glycine, control +0.2% NAC +0.5% glycine. Each diet was randomly assigned to quadruplicate groups of 30 fish (approximately 9.5 g). The weight gain and specific growth rate were significantly increased with the supplementation of NAC and glycine. While they had no effect on feed efficiency feed intake and survival. Glutathion peroxidase (GPx) was increased by NAC and γ-glutamine cysteine synthase (γ-GCS) in plasma were increased by glycine. After the feeding trail, fish were challenged by Streptococcus iniae, fish fed the diet supplemented with NAC obtained significantly higher survival rate after 72 h challenge test. NAC also decreased malonaldehyde (MDA) in liver, increased glutathione S-transferase (GST) activity in plasma, up-regulated mRNA expression of Superoxide dismutase (SOD) and GPx in liver and headkidney. Dietary supplementation of glycine increased the anti-oxidative ability of tilapia through increase anti-oxidative enzyme activity (SOD, glutathione reductase, myeloperoxidase) and up-regulate anti-oxidative gene expression (SOD). Immune ability only enhanced by the supplementation of NAC through increased interleukin-1β (IL-1β) mRNA expression. These results clearly indicated that the supplementation of NAC and glycine can significantly improve the growth performance of tilapia, and NAC also enhance the anti-oxidative and immune capacity of tilapia, glycine could only enhance the anti-oxidative ability. PMID:27235905

  1. Thermal preference predicts animal personality in Nile tilapia Oreochromis niloticus.

    Science.gov (United States)

    Cerqueira, Marco; Rey, Sonia; Silva, Tome; Featherstone, Zoe; Crumlish, Margaret; MacKenzie, Simon

    2016-09-01

    Environmental temperature gradients provide habitat structure in which fish orientate and individual thermal choice may reflect an essential integrated response to the environment. The use of subtle thermal gradients likely impacts upon specific physiological and behavioural processes reflected as a suite of traits described by animal personality. In this study, we examine the relationship between thermal choice, animal personality and the impact of infection upon this interaction. We predicted that thermal choice in Nile tilapia Oreochromis niloticus reflects distinct personality traits and that under a challenge individuals exhibit differential thermal distribution. Nile tilapia were screened following two different protocols: 1) a suite of individual behavioural tests to screen for personality and 2) thermal choice in a custom-built tank with a thermal gradient (TCH tank) ranging from 21 to 33 °C. A first set of fish were screened for behaviour and then thermal preference, and a second set were tested in the opposite fashion: thermal then behaviour. The final thermal distribution of the fish after 48 h was assessed reflecting final thermal preferendum. Additionally, fish were then challenged using a bacterial Streptococcus iniae model infection to assess the behavioural fever response of proactive and reactive fish. Results showed that individuals with preference for higher temperatures were also classified as proactive with behavioural tests and reactive contemporaries chose significantly lower water temperatures. All groups exhibited behavioural fever recovering personality-specific thermal preferences after 5 days. Our results show that thermal preference can be used as a proxy to assess personality traits in Nile tilapia and it is a central factor to understand the adaptive meaning of animal personality within a population. Importantly, response to infection by expressing behavioural fever overrides personality-related thermal choice. PMID:27219014

  2. The influence of wine-distillery waste compost on nitrogen and phosphorus dynamics and uptake by a melon crop in a shallow calcareous soil

    Science.gov (United States)

    Requejo, M. I.; Villena, R.; Ribas, F.; Castellanos, M. T.; Cabello, M. J.; Arce, A.; Cartagena, M. C.

    2012-04-01

    In Mediterranean countries, the large quantity of organic wastes generated by the winery industry constitutes a serious environmental concern, due to its low pH and high content of phenolic compounds. This is accompanied by a seasonal production that makes their management difficult. Winery wastes are characterized by high organic matter contents, low electrical conductivity values and notable contents in macronutrients, so their use as organic amendments is a good management option for improving soil fertility. However, a composting treatment is necessary to convert these organic wastes into more stable, hygienic and humic-rich materials. The aim of this work was to evaluate the effects of the application of exhausted grape marc compost (composed of dealcoholized pulp, skins and stems) as fertilizer in soil nitrogen and phosphorus availability and uptake by a melon crop (Cucumis melo L.). This experiment was carried out from May to September 2011 in Ciudad Real (Spain). This area was designated "vulnerable zone" by the "Nitrates Directive" 91/676/CEE. The soil was a shallow sandy-loam (Alfisol Xeralf Petrocalcic Palexeralfs) with a depth of 0.60 m and a discontinuous petrocalcic horizon between 0.60 and 0.70 m, slightly basic (pH 7.9), poor in organic matter (0.20%), rich in potassium (407 ppm) and with a medium level of phosphorus (19.4 ppm). The experiment had a randomised complete block design, with four treatments consisted of four compost doses: 0 (D0), 6.7 (D1), 13.3 (D2) and 20 T compost ha-1 (D3), in order to determine the optimum dose to ensure nutrient demand, maximizing yield and minimizing nutrient losses. Acknowledgements This project has been supported by INIA-RTA2010-00110-C03-01.

  3. Mechanisms of Oryza sativa (Poaceae) resistance to Tagosodes orizicolus (Homoptera: Delphacidae) under greenhouse condition in Venezuela.

    Science.gov (United States)

    González, Alex; Labrín, Natalia; Alvarez, Rosa M; Jayaro, Yorman; Gamboa, Carlos; Reyes, Edicta; Barrientos, Venancio

    2012-03-01

    Tagosodes orizicolus is one of the main plagues of rice in tropical America causing two types of damages, the direct one, feeding and oviposition effect, and an indirect one, by the transmission of the "Rice hoja blanca virus". During 2006-2007 we carried out research under greenhouse conditions at Fundaci6n Danac, Venezuela, in order to determine the mechanisms of antixenosis, antibiosis and tolerance to T. orizicolus, which could be acting in commercial varieties and advanced lines of the rice genetic breeding programs of INIA and Fundaci6n Danac. The method of free feeding was used for the antixenosis evaluation, whereas the method of forced feeding was used for antibiosis evaluation (effect on survival and oviposition). Additionally, we used the indirect method based on biomass depression to estimate the tolerance. Some of the evaluated traits included: grade of damage, number of insects settling on rice plants, percentage of sogata mortality at the mature state, number of eggs in the leaf midrib and an index of tolerance. The results showed that rice genotypes possess different combinations of resistance mechanisms, as well as different grades of reactions. The susceptible control 'Bluebonnet 50' was consistently susceptible across experiments and the resistant control 'Makalioka' had high antixenosis and high antibiosis based on survival and oviposition. The rest of the genotypes presented lower or higher degrees of antixenosis and antibiosis for survival and oviposition. The genotype 'FD0241-M-17-6-1-1-1-1' was identified with possible tolerance to the direct damage of sogata. PMID:22458212

  4. Depletion of florfenicol amine in tilapia (Oreochromis sp.) maintained in a recirculating aquaculture system following Aquaflor(R)-medicated feed therapy (20 mg/kg BW/d for 10 days)

    Science.gov (United States)

    Gaikowski, Mark P.; Whitsel, Melissa K.; Charles, Shawn; Schleis, Susan M.; Crouch, Louis S.; Endris, Richard G.

    2013-01-01

    Aquaflor® [50% w w−1 florfenicol (FFC)], is approved for use in freshwater-reared warmwater finfish which include tilapia Oreochromis spp. in the United States to control mortality from Streptococcus iniae. The depletion of florfenicol amine (FFA), the marker residue of FFC, was evaluated after feeding FFC-medicated feed to deliver a nominal 20 mg FFC kg−1 BW d−1 dose (1.33× the label use of 15 mg FFC kg−1 BW d−1) to Nile tilapia O. niloticus and hybrid tilapia O. niloticus × O. aureus held in a recirculating aquaculture system (RAS) at production-scale holding densities. Florfenicol amine concentrations were determined in fillets taken from 10 fish before dosing and from 20 fish at nine time points after dosing (from 1 to 240 h post-dosing). Water samples were assayed for FFC before, during and after the dosing period. Parameters monitored included daily feed consumption and biofilter function (levels of ammonia, nitrite and nitrate). Mean fillet FFA concentration decreased from 13.77 μg g−1 at 1-h post dosing to 0.39 μg g−1 at 240-h post dosing. Water FFC concentration decreased from a maximum of 1400 ng mL−1 at 1 day post-dosing to 847 ng mL−1 at 240 h post-dosing. There were no adverse effects noted on fish, feed consumption or biofilter function associated with FFC-medicated feed administration to tilapia.

  5. A teleostan homolog of catalase from black rockfish (Sebastes schlegelii): insights into functional roles in host antioxidant defense and expressional responses to septic conditions.

    Science.gov (United States)

    Elvitigala, Don Anushka Sandaruwan; Priyathilaka, Thanthrige Thiunuwan; Whang, Ilson; Nam, Bo-Hye; Lee, Jehee

    2015-05-01

    Antioxidative defense renders a significant protection against environmental stress in organisms and maintains the correct redox balance in cells, thereby supporting proper immune function. Catalase is an indispensable antioxidant in organisms that detoxifies hydrogen peroxides produced in cellular environments. In this study, we sought to molecularly characterize a homolog of catalase (RfCat), identified from black rockfish (Sebastes schlegelii). RfCat consists of a 1581 bp coding region for a protein of 527 amino acids, with a predicted molecular weight of 60 kD. The protein sequence of RfCat harbored similar domain architecture to known catalases, containing a proximal active site signature and proximal heme ligand signature, and further sharing prominent homology with its teleostan counterparts. As affirmed by multiple sequence alignments, most of the functionally important residues were well conserved in RfCat. Furthermore, our phylogenetic analysis indicates its common vertebrate ancestral origin and a close evolutionary relationship with teleostan catalases. Recombinantly expressed RfCat demonstrated prominent peroxidase activity that varied with different substrate and protein concentrations, and protected against DNA damage. RfCat mRNA was ubiquitously expressed among different tissues examined, as detected by qPCR. In addition, RfCat mRNA expression was modulated in response to pathogenic stress elicited by Streptococcus iniae and poly I:C in blood and spleen tissues. Collectively, our findings indicate that RfCat may play an indispensable role in host response to oxidative stress and maintain a correct redox balance after a pathogen invasion.

  6. Molecular cloning and expression analysis of interleukin (IL)-15 and IL-15 receptor α from rock bream, Oplegnathus fasciatus.

    Science.gov (United States)

    Bae, Jin-Sol; Shim, Sang Hee; Hwang, Seong Don; Kim, Ju-Won; Park, Dae-Won; Park, Chan-Il

    2013-10-01

    Mammalian interleukin (IL)-15 plays an important role in the activation of CD8(+) T cells and natural killer (NK) cells along with its receptor α (IL-15Rα). To understand the potential roles of IL-15 and IL-15Rα in fish, we identified IL-15 and IL-15Rα cDNA from rock bream (Oplegnathus fasciatus) and investigated their gene expression profiles after bacterial and viral infection. Coding regions of rock bream (Rb) IL-15 and RbIL-15Rα cDNAs were 534 and 402 bp encoding 177 and 133 amino acid residues, respectively. The sushi domain of IL-15Rα was highly conserved between rock bream and other species. Unlike other IL-15Rαs, RbIL-15Rα does not have a transmembrane region. Gene expression of RbIL-15 and RbIL-15Rα was widely expressed in different tissues of healthy fish, especially immune-related tissues. RbIL-15 and RbIL-15Rα were highly induced in the kidney and spleen after infection with Edwardsiella tarda, Streptococcus iniae and red seabream iridovirus. Gene expression patterns of RbIL-15 and RbIL-15Rα were similar in the kidney and spleen after pathogen infection. However, these genes were differentially induced in the liver after pathogen infection. These results suggest that the different responses of RbIL-15 and RbIL-15Rα to pathogen infection may be induced by different tissues or cell types. PMID:23911652

  7. EVALUACIÓN DE DENSIDADES DE SIEMBRA EN Solanum sessiliflorum dunal “COCONA” Y SU EFECTO EN EL RENDIMIENTO DE FRUTO

    Directory of Open Access Journals (Sweden)

    Rodrigo Gonzáles Vega

    2012-12-01

    Full Text Available La cocona es una especie vegetal nativa de América Tropical, cuyos frutos maduros son ricos en hierro, vitamina B5 (Niacina, se utilizan en la preparación de jugos, refrescos, jarabes y ensaladas. El estudio se realizó en el Campo Experimental “El Dorado” de Estación Experimental San Roque, del INIA Loreto con el objetivo principal de determinar el mejor distanciamiento para establecer plantaciones comerciales de Solanum sessiliflorum dunal “Cocona”, en condiciones de suelos no inundables o de altura. Se evaluaron cuatro densidades de siembra 2,0m x 2,0m; 1,5m x 1,5m, 2,0m x 1,0 y 1,5m x 1,0m. Las variables evaluadas fueron el número de frutos, peso de frutos y rendimiento de frutos por cada tratamiento en estudio, el diseño experimental utilizado fue Bloques Completos al Azar (DBCA, con cuatro repeticiones, los datos fueron analizados mediante el Análisis de Variancia y Prueba Estadística de Tukey, usando el software SPSS, versión 10. De los resultados Obtenidos se determinó que la densidad 1,5m x 1,0m (6 666 plantas/ha, produjo el mejor rendimiento con 14 600 kg/ha de fruto, superando significativamente a los tratamientos 2m x 1,0, 1,5m x 1,5m y 2,0 x 2,0m, que alcanzaron rendimientos de fruto de 12020, 11 330 y 10 570 kg/ha, respectivamente. Se concluye que la densidad de siembra influye en el rendimiento de fruto, así a menor número de plantas, mayor número y peso de fruto por planta se obtiene un menor rendimiento de fruto/ha.

  8. Effect of the time of application of phosphorus fertilizer on yield and quality parameters of melon crop amended with winery waste compost.

    Science.gov (United States)

    Requejo Mariscal, María Isabel; Cartagena, María Carmen; Villena Gordo, Raquel; Arce Martínez, Augusto; Ribas Elcorobarrutia, Francisco; Jesús Cabello Cabello, María; Castellanos Serrano, María Teresa

    2016-04-01

    In Spain, drip irrigation systems are widely used for horticultural crop production. In drip irrigation systems, emitter clogging has been identified as one of the most important concerns. Clogging is closely related to the quality of the irrigation water and the structure of the emitter flow path, and occurs as a result of multiple physical, biological and chemical factors. So, the use of acid fertilizers (e.g. phosphoric acid) in these systems is common to avoid the emitter clogging. Moreover, in this country the use of exhausted grape marc compost as source of nutrients and organic matter has been identified as a good management option of soil fertility, especially in grape-growing areas with a large generation of wastes from the wine and distillery industries. The purpose of this work was to study the effect of the time of application of phosphorus fertilizer with fertirrigation in a melon crop amended with winery waste compost on yield and quality parameters. During two years, the melon crop was grown under field conditions and beside the control treatment, three doses of compost were applied: 6.7, 13.3 and 20.0 t ha-1. All the compost treatments received 120 kg ha-1 of phosphorus fertilizer (phosphoric acid) for the season varying the time of application: The first year phosphorus application started after male and female flowering, and the second year the application started before flowering. Yield and quality parameters were evaluated to assess the suitability of these practices. Acknowledgements: This project has been supported by INIA-RTA2010-00110-C03. Keywords: Phosphorus fertilizer, exhausted grape marc compost, melon crop, yield and quality parameters.

  9. Inducción de la biosíntesis local de antocianinas en frutos de Myrciaria dubia mediante lesiones mecánicas

    Directory of Open Access Journals (Sweden)

    Daniel Motta-Santillán

    2015-01-01

    Full Text Available En las plantas las antocianinas cumplen roles fundamentales en sus interacciones con el medio ambiente, son una de las líneas de defensa contra los radicales libres, la radiación ultravioleta y el ataque de patógenos. Observaciones realizadas muestran que los frutos de M. dubia acumulan antocianinas alrededor de lesiones mecánicas probablemente causadas por insectos. Por tanto, hemos probado la hipótesis que produciendo lesiones mecánicas en frutos verdes de M. dubia se induce la biosíntesis local de antocianinas. De tres plantas en fructificación de la colección de germoplasma del INIA se seleccionaron aleatoriamente 40 frutos verdes (25 ± 5 mm de Ø y se causaron lesiones mecánicas con estilete o bisturí al 75% de ellos. Posteriormente, se registró el porcentaje de acumulación de antocianinas por área superficial del fruto a las 0, 24, 48 y 72 horas. Se observó acumulación de antocianinas en ~10% (24 horas, ~30% (48 horas y ~70% (72 horas del área superficial de los frutos. En conclusión, las lesiones mecánicas causadas en frutos de M. dubia inducen la biosíntesis local de antocianinas, probablemente como un mecanismo de defensa efectivo desarrollado por esta especie. La comprensión de los mecanismos moleculares implicados en esta respuesta fisiológica nos permitirá establecer las bases para la mejora genética de esta especie.

  10. Fluctuación diurna del contenido de vitamina C en hojas de Myrciaria dubia “camu camu”

    Directory of Open Access Journals (Sweden)

    Franz Correa Meléndez

    2013-12-01

    Full Text Available Myrciaria dubia “camu camu” es un frutal del trópico amazónico caracterizado por sus frutos con gran contenido de vitamina C, siendo considerado un producto importante del país. Sin embargo, hay pocos reportes sobre el metabolismo y transporte de vitamina C en esta especie. El objetivo de la investigación fue determinar la fluctuación diurna del contenido de vitamina C en hojas de M. dubia “camu camu”. Las hojas se colectaron de la colección de germoplasma de “camu camu” del INIA a las 2, 6, 10, 14, 18 y 22 horas; de cuatro plantas y en tres fechas diferentes. La vitamina C fue extraída de las hojas con un método estandarizado en el laboratorio y se cuantificó mediante espectrofotometría a 530nm, previa reacción con 2,6-diclorofenolindofenol. Los resultados muestran que el contenido de vitamina C de las hojas de “camu camu” fue en promedio 231±35 mg vitamina C/100g de hoja. Además, el contenido de vitamina C en las hojas del “camu camu” mostró fluctuación diurna, siendo menor a las 6 horas y mayor a las 2 y 14 horas del día. También, se registraron concentraciones intermedias en horas de menor o ausencia de radiación solar (235±17 y 237±35 mg vitamina C/100g de hoja a las 18 y 22 horas respectivamente. Se concluye que existe alto contenido de vitamina C en las hojas del “camu camu” y que este contenido presenta fluctuación diurna.

  11. The effects of combined dietary probiotics Lactococcus lactis BFE920 and Lactobacillus plantarum FGL0001 on innate immunity and disease resistance in olive flounder (Paralichthys olivaceus).

    Science.gov (United States)

    Beck, Bo Ram; Kim, Daniel; Jeon, Jongsu; Lee, Sun-Min; Kim, Hui Kwon; Kim, Oi-Jin; Lee, Jae Il; Suh, Byung Sun; Do, Hyung Ki; Lee, Kwan Hee; Holzapfel, Wilhelm H; Hwang, Jee Youn; Kwon, Mun Gyeong; Song, Seong Kyu

    2015-01-01

    The effects of a dietary probiotic mixture containing Lactococcus (Lc.) lactis BFE920 isolated from bean sprout and autochthonous Lactobacillus (Lb.) plantarum FGL0001 originally isolated from the hindgut of olive flounder (Paralichthys olivaceus) were investigated for the purpose of improving the probiotic effects of Lc. lactis BFE920 on the olive flounder. The immunostimulatory, disease protective, and weight gain effects of Lc. lactis BFE920 were significantly improved when olive flounder (average weight 37.5±1.26 g) were fed the probiotic mixture (log10 7.0 CFU each/g feed pellet) for 30 days. Flounder fed the mixture showed improved skin mucus lysozyme activity and phagocytic activity of innate immune cells compared to flounder fed a single probiotic agent or a control diet. While the levels of neutrophil activity in flounder fed the single probiotic agent or the mixture were similar, they were significantly higher than levels in a control group. Additionally, probiotic-fed flounder showed significantly increased expressions of IL-6, IL-8, and TNF-α in the intestine compared to the control group. Following a 30-day period of being fed probiotics or a control diet, the olive flounder were challenged with an i.p. injection of Streptococcus iniae (log10 6.0 CFU/fish). The groups fed the mixed probiotics, Lc. lactis BFE920, Lb. plantarum FGL0001, and the control diet had survival rates of 55%, 45%, 35%, and 20%, respectively. Flounder fed the probiotic mixture gained 38.1±2.8% more body weight compared to flounder fed the control diet during the 30-day study period. These data strongly suggest that a mixture of Lc. lactis BFE920 and Lb. plantarum FGL0001 may serve as an immunostimulating feed additive useful for disease protection in the fish farming industry. PMID:25449382

  12. Embryogenesis in Oak species. A review

    Directory of Open Access Journals (Sweden)

    Aranzazu Gomez-Garay

    2014-08-01

    Full Text Available Aim of study: A review on the propagation methods of four Quercus species, namely Q. suber, Q. robur, Q. ilex and Q. canariensis, through somatic embryogenesis and anther embryogenesis are presented.Area of study: The study comprises both Mediterranean and Atlantic oak species located in Spain.Material and Methods: Somatic embryogenesis was induced on immature zygotic embryos of diverse oak species, permitting the multiplication of half-sib families. Induction of haploid embryos and doubled haploids was assayed in both Q. suber and Q. ilex by temperature stress treatments of anthers containing late vacuolated microspores. The haploid origin of the anther embryos has been evaluated by quantitative nuclear DNA analysis through flow cytometry and by DNA microsatellite markers. Genetic transformation of cork oak has also been performed by means of Agrobacterium tumefaciens vectors. Proteomic analysis has been conducted to screen the diverse protein profiles followed by in vitro derived embryos during their development.Research highlights: Successful plant regeneration from both somatic and haploid embryos has been achieved. In the particular case of cork oak, doubled-haploid plants were obtained. Plantlets regenerated from selected parent trees through somatic embryogenesis were acclimated in the greenhouse and in the nursery, and were planted in an experimental plot in the field. Preliminary evaluation of the cork quality of the plants showed a good heritability correlation with the parent trees. This article revises the work of and is dedicated to Dr. M.A. Bueno, who devoted much of her professional life to the research on Biotechnology and Genetics of forest species, leading the Laboratory of Forest Biotechnology at the Spanish Institute of Agronomic Research (INIA.Key words: anther embryogenesis; microspore; pollen; Quercus canariensis; Quercus ilex; Quercus robur; Quercus suber; somatic embryogenesis. 

  13. Las arañas en agroecosistemas: bioindicadores terrestres de calidad ambiental

    Directory of Open Access Journals (Sweden)

    Miguel Simó

    2011-12-01

    Full Text Available Las arañas son predadores generalistas en ecosistemas terrestres, ampliamente utilizadas en estudios de biodiversidad. En el último siglo, el paisaje de Uruguay se transformó en un mosaico de áreas formadas por ambientes naturales y agroecosistemas. ¿Qué nivel de cambio se espera hallar en la comunidad de arañas si las áreas de estudio están próximas? ¿Qué porcentaje de especies nativas serán reemplazadas con la plantación? ¿Cómo afecta esto a la biodiversidad local? Se realizó un estudio en cuatro áreas vecinas en INIA Las Brujas, Canelones: un bosque ribereño, un bosque de “Espinillo”, una plantación de Eucalyptus globulus y una pradera artificial de Trifolium pratense y Avena sativa. Las arañas fueron capturadas con trampas de caída, trampas de tronco, segado, batido de follaje y colecta manual nocturna. Un total de 3.023 adultos fueron colectados. La mayor abundancia y riqueza de especies se observó en el bosque ribereño. A pesar de la proximidad de los sitios estudiados, se observó un alto reemplazo de especies, especialmente entre áreas naturales y agroecosistemas. Los protocolos de colecta utilizados podrían ser útiles para evaluar el impacto de agroecosistemas con diferentes tipos de manejos sobre la biodiversidad local y su aplicación en la certificación de calidad ambiental.

  14. Increasing nitrogen rates in rice and its effect on plant nutrient composition and nitrogen apparent recovery

    Directory of Open Access Journals (Sweden)

    Juan Hirzel

    2013-12-01

    Full Text Available Rice (Oryza sativa L. is one of the essential foods of the human diet; advances in agronomic crop management can improve productivity and profitability as well as reduce adverse environmental impacts. Nitrogen rates in Chile are generally based on crop yield without considering other agronomic factors. The objective of this experiment was to determine the effect of increasing N rates on plant nutrient composition and N apparent recovery in rice cultivated in five different locations in Chile. The five sites located in central Chile belong to one of the following soil orders: Inceptisol, Alfisol, and Vertisol; they were cropped in field conditions with 'Zafiro-INIA' rice fertilized with 0, 80, and 160 kg N ha-1. Whole-plant total DM, macronutrient composition, and N apparent recovery efficiency (NARE were determined at grain harvest. Results indicate that all evaluated parameters, with the exception of K concentration, were affected by the soil used. Nitrogen rates only affected total DM production and P, K, and Mg concentrations in plants. Phosphorus and K response decreased when N was added to some soils, which is associated with its chemical properties. Magnesium concentration exhibited an erratic effect, but it was not affected by the N rate in most soils. Nitrogen apparent recovery efficiency was not affected by the N rate and accounted for approximately 49% and 41% for 80 and 160 kg N ha-1, respectively. Macronutrient composition was 5.1-7.7 g N, 1.3-1.8 g P, 5.4-10.8 g K, 1.68-2.57 g Ca, and 0.81-1.45 g Mg kg-1 of total DM.

  15. Effect of different mulch materials on the soil dehydrogenase activity (DHA) in an organic pepper crop

    Science.gov (United States)

    Moreno, Marta M.; Peco, Jesús; Campos, Juan; Villena, Jaime; González, Sara; Moreno, Carmen

    2016-04-01

    The use biodegradable materials (biopolymers of different composition and papers) as an alternative to conventional mulches has increased considerably during the last years mainly for environmental reason. In order to assess the effect of these materials on the soil microbial activity during the season of a pepper crop organically grown in Central Spain, the soil dehydrogenase activity (DHA) was measured in laboratory. The mulch materials tested were: 1) black polyethylene (PE, 15 μm); black biopolymers (15 μm): 2) Mater-Bi® (corn starch based), 3) Sphere 4® (potato starch based), 4) Sphere 6® (potato starch based), 5) Bioflex® (polylactic acid based), 6) Ecovio® (polylactic acid based), 7) Mimgreen® (black paper, 85 g/m2). A randomized complete block design with four replications was adopted. The crop was drip irrigated following the water demand of each treatment. Soil samples (5-10 cm depth) under the different mulches were taken at different dates (at the beginning of the crop cycle and at different dates throughout the crop season). Additionally, samples of bare soil in a manual weeding and in an untreated control were taken. The results obtained show the negative effect of black PE on the DHA activity, mainly as result of the higher temperature reached under the mulch and the reduction in the gas interchange between the soil and the atmosphere. The values corresponding to the biodegradable materials were variable, although highlighting the low DHA activity observed under Bioflex®. In general, the uncovered treatments showed higher values than those reached under mulches, especially in the untreated control. Keywords: mulch, biodegradable, biopolymer, paper, dehydrogenase activity (DHA). Acknowledgements: the research was funded by Project RTA2011-00104-C04-03 from the INIA (Spanish Ministry of Economy and Competitiveness).

  16. SKIN MUCUS OF RABBITFISH (SIGANUS ORAMIN) IS LETHAL TO CRYPTOCARYON IRRITANS AND SOME OTHER PATHOGENIC ORGANISMS%黄斑蓝子鱼皮肤黏液对刺激隐核虫及一些病原菌的抑杀作用

    Institute of Scientific and Technical Information of China (English)

    黎睿君; 刘芳; 王方华; 李安兴

    2013-01-01

    Cryptocaryon irritans, an important marine ciliate protozoan that causes a disease known as "marine white spot disease", has already become one of the most harmful parasites in Chinese marine aquaculture industry. Previous studies indicated that the rabbitfish (Siganus oramin) showed low susceptibility to C. irritans infections, and the serum of rabbitfish had killing effect against C. irritans. Practically, the skin mucus of fish has been considered to be the first defense against infections caused by various pathogens. Whether the skin mucus of rabbitfish has the same parasiticidal activity is the main research purpose of this paper. In this study, we compared the antiparasitic and antibacterial activities of skin mucus from S. oramin, Epinephelus coioides, Sciaemops ocellatus, Plectorhinchus cinctus and Trachinotus ovatus. The parasite-killing effects of skin mucus were tested by immobilization assay for three species of parasites, C. irritans, I. multifiliis, and T. brucei brucei. The antimicrobial properties of skin mucus were determined using the agar diffusion method and the minimum inhibitory concentration for six species of bacteria (Staphylo coccus aureus, Bacillus subtilis, Streptococcus iniae, Escherichia coli, Vibrio cholera, Aeromonas sobria, Edwardsiella tarda, Photobacterium damselae subsp. and Vibrio alginolyticus) and two species of fungi (Aspergillus niger and Gibberella saubinetii) were also examined. We found that compared with the skin mucus isolated from E. coioides, S. ocellatus, P. cinctus and T. ovatus, the rabbitfish skin mucus was lethal to C. irritans, I. multifiliis, and T. brucei brucei. The minimum parasiticidal concentration (MPC) of rabittfish skin mucus for C. irritans, I. multifiliis and T. brucei brucei were 4.0, 5.0, and 3.0 mg/mL, respectively. After the theronts of C. irritans and /. multifiliis were co-incubated with the skin mucus of rabbit-fish, we observed the cilia falling off, body swelling, outer membrane rupture and

  17. Vascular reconstruction of variant donor renal arteries in 14 living-related kidney transplant cases%14例亲属活体供肾动脉变异的血管重建

    Institute of Scientific and Technical Information of China (English)

    刘龙山; 王长希; 陈立中; 邓素雄; 费继光; 邱江; 滕立臣

    2009-01-01

    (TRA).Branch arteries of ED were injured when kidneys were harvested,and vascular reconstruction was performed with recipients' internal iliac artery(InlA)and its branches,or inferior epigastric artery(IEA).In 10 cases of DRA,vascular reconstruction was performed with InIA and its branches in 4 patients or with IEA in 3 patients.Short RA was end-to-side anastomosed to longer RA in one DRA patient.accessory RA was end-to-side anastomosed to main RA in one DRA patient,and 2 RAs were end-to-side anastomosed to externaliliac artery(EIA) in another one.Vascular reconstruction was performed with InIA and its branches in one TRA patient,and accessory RA was end-to-side anastomosed to main RA after ligating the minor branches in another TRA patient.In all the 14 patients,RA and InIA trunk were respectively or jointly anastomosed to EIA in an end-to-side manner.Results After transplantation,sufficient and uniform blood supply to grafts was observed without RA obstruction.Graft function fluently recovered in 12 patients,in one of whom acute rejection(AR)occurred at day 14.One patient showed slow decrease of serum creatinine(SCr),and AR occurred in another one right after KTx The TRA patient with severe artherosclerosis developed polar renal artery embolism.whose SCr increased and maintained at 170 μmol/L without medical intervention.During the follow-up to July 2008,the remaining patients showed normal blood flow and sufficient blood supply.Conclusions Vascular reconstruction of variant donor renal artery can be performed with its own vascular condition after kidney harvest,or recipient's internal iliac artery and its branches or inferior epigastric artery,which is an effective way to obtain good graft function.For patients with artherosclerosis,artery embolism should be concerned when tiny renal artery is anastomosed.

  18. Impact of climate change on olive crop flowering at southern Spain

    Science.gov (United States)

    Gabaldón-Leal, Clara; Ruiz-Ramos, Margarita; de la Rosa, Raúl; León, Lorenzo; Rodríguez, Alfredo; Belaj, Angjelina; Lorite, Ignacio

    2016-04-01

    Andalusia region (Southern Spain) is the largest olive oil producer in the world with 40% of the total production (1.1millions tons; FAOSTAT, 2013). Predicting flowering dates of olive under future climate conditions has a critical importance as flowering is a critical stage for olive as heat and water stresses during this period have a significant impact on final olive oil yield. The aim of this study was to evaluate the olive flowering dates under future climate conditions. Climate data for the future period was obtained from the ENSEMBLES European Project (http://www.ensembles-eu.org/) with a bias correction in temperature and precipitation with regard with the SPAIN02 dataset (Herrera et al., 2012) (ENS-SP). Flowering of ten different olive cultivars were evaluated under current and forced (greenhouse) climate conditions, applying the bias in temperatures comparing the current period (1981-2010) with the future period (2071-2100) to the current conditions in Cordoba (Andalusia, Spain). These observations allowed obtaining a flowering approach which was applied to the whole Andalusia region. The results showed an average advance in flowering dates about 16 days at the end of the 21st century. With these results different areas within Andalusia region were classified based on the vulnerability caused by the lack of chilling hours accumulation (coastal areas) or by high temperatures during flowering (north and northeast regions). This study has been supported by the project RTA2014-00030-00-00 funded by INIA and FEDER 2014-2020 "Programa Operativo de Crecimiento Inteligente" and IFAPA project AGR6126 from Junta de Andalucía, Spain. FAOSTAT (2013) Food And Agriculture Organization Of The United Nations. Available at http://faostat3.fao.org/browse/Q/QD/E. Accessed 07 January 2016 Herrera S, Gutiérrez JM, Ancell R, Pons MR, Frías MD, Fernández J. 2012. Development and analysis of a 50-year high-resolution daily gridded precipitation dataset over Spain (Spain02

  19. ДИКОРАСТУЩИЕ ВИДЫ МЯТЫ КАК НОСИТЕЛИ ГЕНОВ УСТОЙЧИВОСТИ К НЕБЛАГОПРИЯТНЫМ ФАКТОРАМ ОКРУЖАЮЩЕЙ СРЕДЫ

    OpenAIRE

    БУГАЕНКО ЛЮДМИЛА АЛЕКСАНДРОВНА

    2015-01-01

    Показано, что дикорастущие виды мяты могут являться носителями генов морозоустойчивости и устойчивости к ржавчине, которая вызывается грибом Рuссinia menthae Pers. Перспективным для создания устойчивых сортов является метод межвидовой гибридизации с использованием культивируемых видов Mentha piperita L. и Mentha canadensis L., а также дикорастущих видов Mentha aquatica L., Mentha spicata L. и Mentha longifolia (L.) Nathh. Выявлены доноры устойчивости к ржавчине M. canadensis K60 (4п) и M. aqu...

  20. Molecular cloning and characterization of a new G-type lysozyme gene (Ec-lysG) in orange-spotted grouper, Epinephelus coioides.

    Science.gov (United States)

    Wei, Shina; Huang, Youhua; Huang, Xiaohong; Cai, Jia; Wei, Jingguang; Li, Pengfei; Ouyang, Zhengliang; Qin, Qiwei

    2014-10-01

    Lysozyme acts as an innate immunity molecule against pathogen infection. In this study, a new G-type lysozyme gene with a typical G-type lysozyme domain (designated as Ec-lysG) was cloned and characterized from the orange-spotted grouper, Epinephelus coioides. The full-length Ec-lysG cDNA contains 1419 bp and encodes a 256-residue protein containing a 25-residue signal peptide at the N-terminus. BLAST analysis reveals Ec-lysG shares 64% identity with Siniperca chuatsi, but 63% to another reported G-type lysozyme from orange-spotted grouper (OSG-lysG). The genomic DNA of Ec-lysG contains four exons and three introns, with a total length of 2062 bp. An amino acid sequence alignment showed that Ec-lysG shares the fundamental structural features of G-type lysozyme, including the catalytic residues, substrate binding sites, and soluble lytic transglycosylase domain. Quantitative PCR showed that Ec-lysG transcript is most abundant in the head kidney, and less abundant in the heart. The expression of Ec-lysG was differentially upregulated in the head kidney after stimulation with lipopolysaccharide, Vibrio alginolyticus, and Singapore grouper iridovirus (SGIV). A subcellular localization analysis showed that Ec-lysG is distributed predominantly in the cytoplasm. Recombinant Ec-lysG (rEc-lysG) has optimal activity at pH 7.5 and 35°C. rEc-lysG showed lytic activities against Gram-positive bacterium Streptococcus iniae, Staphylococcus aureus, and Micrococcus lysodeikticus, and the Gram-negative bacterium V. alginolyticus. Scanning electron microscopy (SEM) showed that rEc-lysG acts on M. lysodeikticus cell walls. The overexpression of Ec-lysG in grouper cells did not significantly delay the occurrence of the cytopathic effect (CPE) induced by SGIV, and did not inhibit viral gene transcription. In conclusion, Ec-lysG might be a potent antibacterial protein, with a role in innate immunity.

  1. Yield, nutrient utilization and soil properties in a melon crop amended with wine-distillery waste compost

    Science.gov (United States)

    Requejo Mariscal, María Isabel; Villena Gordo, Raquel; Cartagena Causapé, María Carmen; Arce Martínez, Augusto; Ribas Elcorobarrutia, Francisco; Jesús Cabello Cabello, María; Castellanos Serrano, María Teresa

    2014-05-01

    . With this information, an integrated analysis was carried out with the aim to evaluate the suitability of this compost as organic amendment. Acknowledgements: This project has been supported by INIA-RTA2010-00110-C03-01

  2. Design and synthesis of isoniazide mimetic conjugated with DTPA, potential ligand of novel radiopharmaceutical and contrast agent for medical imaging, Bis (amide) of diethyelene triaminepentaacetic acid: DTPA-Bis(INH)

    International Nuclear Information System (INIS)

    The structure of isonicotinic acid hydrazide was coupled to diethylenetriaminepentaacetic acid (DTPA) via amide linkage. The Overall yield of three steps synthesis starting from DTPA is above 80%. The new ligand binds with infection site through specific interaction with gene iniA, iniB and iniC under pathological conditions. In particular, complexation of DTPA moiety with 99mTc and Gd+III showed excellent results as metallopharmaceutical for medical imaging. Primary object of the present invention is to propose a novel INH derivative based on DTPA, which form stable complexes with most of lanthanides and transition metals in periodic table. Another object of the present invention is to introduce a chelating group without compromising its biological activity for early diagnosis of infection using nuclear medicine and MR techniques. The DTPA-Bis(INH) was synthesized in high yield using simple synthetic procedure. Radiochemical purity was ascertained chromatographically using different solvent system. Blood kinetics in rabbits and biodistribution in mice was studied. The ability of DTPA-Bis(INH) to target infection site in vivo was assessed in gamma scintigraphic studies of normal rabbit and a rabbit with induced tuberculosis. The DTPA-Bis(INH) was characterized by Mass spectroscopy in ESI positive mode, M+H+ was found to be 632.2.The complex was successfully labeled with 99mTc radionuclide with more than 95% labeling efficiency.It was found stable up to 24h. Blood kinetics showed rapid first pass clearance with biological half life t1/2 (F) = 11 min. Imaging of a normal rabbit and a rabbit with induced tuberculosis was carried out. An appreciable activity was visualized in liver and kidneys. In diseased rabbit similar pattern was observed with the accumulation of activity at the tubercular site at 24 h post injection. Biodistribution revealed major accumulation in liver 6.30±0.58%ID/g at 1 h and 6.25±0.11 at 4h and in kidneys 7.65±0.52%ID/g at 1h and 6.70±0

  3. Streptococcus agalactiae: HASTA AHORA EL ÚNICO Streptococcus PATÓGENO DE TILAPIAS CULTIVADAS EN COLOMBIA

    Directory of Open Access Journals (Sweden)

    AP Jiménez

    2007-01-01

    Full Text Available Las estreptococosis son un conjunto de enfermedades ocasionadas por un grupo de cocos Gram positivos con similares signologías que involucran distintos órganos en los individuos afectados. La identiÀcación precisa de cada uno de estos microorganismos no se logra de manera deÀnitiva por los métodos tradicionales microbiológicos, por lo que se debe acudir a otro tipo de metodologías como las técnicas de biología molecular. En 1999 se identiÀcó por primera vez en Colombia la estreptococosis en híbridos de tilapias de cultivo. La pos-terior secuenciación del ADN de distintos aislamientos obtenidos de varias regiones del país demostró un 98,8% de aÀnidad con el Streptococcus agalactiae. El presente estudio pretende deÀnir si hasta la fecha existe solo esta especie de Streptococcus en el país causan-do infección o enfermedad en tilapias de cultivo o, por el contrario, son varias las especies que intervienen en los cuadros infecciosos. Se evaluaron aislamientos de tejidos, de agua y fango de los sitios de cultivo, así como de lugares de expendio de tilapia roja (Oreochromissp., utilizando técnicas microbiológicas, inmunoperoxidasa indirecta (IPI y PCR, espe-cíÀcas para el aislamiento e identiÀcación del S. agalactiae. Los resultados del presente estudio demostraron que hasta la fecha en el país únicamente se ha identiÀcado la especie S. agalactiae causando infección o enfermedad en tilapias. No tenemos evidencia de que otros Streptococcus reportados internacionalmente como S. iniae y otros Gram positivos causen estreptococosis en Colombia.La tilapia parece ser el principal reservorio del S. agalactiae en el país, y el riesgo zoonótico, aunque existe, es mínimo si se toman las medidas apropia-das de bioseguridad.

  4. Molecular aspects, genomic arrangement and immune responsive mRNA expression profiles of two CXC chemokine receptor homologs (CXCR1 and CXCR2) from rock bream, Oplegnathus fasciatus.

    Science.gov (United States)

    Umasuthan, Navaneethaiyer; Wan, Qiang; Revathy, Kasthuri Saranya; Whang, Ilson; Noh, Jae Koo; Kim, Seokryel; Park, Myoung-Ae; Lee, Jehee

    2014-09-01

    that of OfCXCR1. In addition, the magnitude of the OfCXCR1 and OfCXCR2 transcripts in head kidney and spleen was differentially increased after the in vivo administration of immune stimulants, LPS and poly I:C and in the infection models injected with rock bream irido virus, Edwardsiella tarda and Streptococcus iniae. These lines of evidence suggest that these receptors may play an important role(s) in immune responsive signaling during pathogenesis of rock bream.

  5. Aplicación del coeficiente de inconsistencia como criterio de comparación entre mediciones Convencionales y Automáticas de lluvia diaria

    Directory of Open Access Journals (Sweden)

    Eva Colotti B.

    2003-01-01

    Full Text Available En Venezuela, los organismos que administran redes meteorológicas como el Ministerio del Ambiente y de los Recursos Naturales (MARN, la Fuerza Aérea Venezolana (FAV, la Armada Venezolana y Instituto Nacional de Investigaciones Agrícolas (INIA y Electrificación del Caroní (CVG-Edelca, entre otros, han iniciado la sustitución de los equipos convencionales de medición por mecanismos automáticos. Sin embargo, ésta sustitución instrumental sería procedente una vez que se hayan comparado simultáneamente las dos sucesiones de medidas, dado que, al evaluar la situación descrita, y haberse decidido eliminar el instrumental convencional, se genera un nuevo conjunto de datos que debería acoplarse a los registros antiguos medidos convencionalmente. Así, se propone un criterio para decidir si es necesario hacer ajustes a los nuevos datos automáticos con respecto a los convencionales o, equivalentemente, si es necesario posponer la eliminación del dispositivo convencional hasta que haya certeza en que las mediciones automáticas son fiables. El Error Cuadrático Medio (ECM fue seleccionado como un índice base en la toma de la decisión respectiva, habiéndose demostrado que ECM = V(X +V(X* - 2 C(X,X*, donde ECM = V(X-X* = varianza de las diferencias de mediciones automáticas y convencionales, V(X = varianza de la medición convencional, V(X* = varianza de la medición automática y C(X,X* = covarianza entre mediciones convencional y automática. Bajo la hipótesis de que E(X = E(X*, se concluye en que el cociente V(X-X* [V(X + V(X*], cuantifica el grado de inconsistencia entre los conjuntos muestrales provenientes de mediciones convencional y automática. Este cociente, denominado Coeficiente de Inconsistencia (acrónimo, CI, varía entre valores 0 (inconsistencia nula o consistencia máxima y 1 (inconsistencia máxima o consistencia nula y permitirá medir en el grado en que mediciones simultáneas bajo distintos criterios de medición son

  6. Depletion of florfenicol amine, marker residue of florfenicol, from the edible fillet of tilapia (Oreochromis niloticus x O. niloticus and O. niloticus x O. aureus) following florfenicol administration in feed

    Science.gov (United States)

    Gaikowski, M.P.; Mushtaq, M.; Cassidy, P.; Meinertz, J.R.; Schleis, S.M.; Sweeney, D.; Endris, R.G.

    2010-01-01

    Aquaflor??, a 50% feed premix containing the broad spectrum antibacterial agent florfenicol is available globally to control mortality associated with economically significant systemic bacterial diseases of fish. Florfenicol (FFC) is effective in controlling mortality associated with Streptococcus iniae in tilapia Oreochromis sp. when administered in medicated feed at a dose of 15 mg/kg bodyweight (BW)/d for 10 consecutive days. Our objective was to characterize the depletion of the FFC marker residue, florfenicol amine (FFA), from the edible tissue of market-weight Nile tilapia O. niloticus x O. niloticus and hybrid tilapia O. niloticus x O. aureus offered feed medicated with FFC at a nominal dose rate of 15 mg/kg BW/d for 12 days. Near market-weight tilapia were obtained from a commercial tilapia farm, distributed to 2 single pass (one for Nile tilapia and one for hybrid tilapia), flow-through systems and maintained at 27 ??C under a 15 h light:9 h dark photoperiod over a 41-d pre-dosing period. During the dosing period, tilapia were offered feed medicated with FFC at a concentration of 1.479 g/kg at 1% BW daily divided in three equal offerings. The initial 10-d dosing period was extended to 12 d because one tank did not consume > 75% of the feed offered during the first two dosing days. The total dose consumed by fish in each of the 2 tanks ranged from 147 to 167 mg/kg. Once during the pre-dose period and on days 1, 2, 4, 7, 14, 21, and 28 of the post-dose period, groups of fish were indiscriminately removed from each tank, measured for weight and length, scaled, filleted, and the skin-on fillets stored at <-70 ??C. Frozen fillets were individually homogenized, extracted, and FFA concentration was determined by high-performance liquid chromatography with UV detection. Florfenicol amine is rapidly eliminated from tilapia fillet after withdrawal from medication and depletion followed first-order kinetics with an estimated half-life of 2.32 d. The FFA tolerance

  7. ELEMENTOS ESTRATÉGICOS DE LA GESTIÓN DEL CONOCIMIENTO ORGANIZACIONAL PARA LA INNOVACIÓN. CASO: RED DE AGROMETEOROLOGÍA

    Directory of Open Access Journals (Sweden)

    Barlin Orlando Olivares

    2016-06-01

    Full Text Available RESUMEN Esta experiencia docente tiene como objetivo proponer un modelo de gestión del conocimiento organizacional para el programa de capacitación desarrollado por el departamento de agrometeorología del Instituto Nacional de Investigaciones Agrícolas (INIA del estado Anzoátegui, Venezuela. Se utilizó el protocolo de solución de problemas, derivado de la aplicación de ciclos de aprendizaje, denominado TADIR (Traducción, Análisis, Diseño, Implementación y Revisión. Se estableció la diversificación de las formas de promover las actividades, tareas, resultados, productos generados por el proyecto, antes, durante y posterior a su ejecución, de manera fácil y completa, así como la conciencia en la población sobre las bondades de la agrometeorológica y su importancia para el desarrollo agrícola del país. Los resultados de esta investigación pueden estar al servicio de la misma organización de manera directa e indirecta y de otras que quieran asumir el reto de contribuir al proceso de transformación que vive la nación. ABSTRACT This teaching experience seeks to propose an organizational knowledge management model for the training program developed by the Agrometeorology Department of the National Institute for Agricultural Research (INAI, in Spanish of the State of Anzoátegui, Venezuela. The problem solution protocol deriving from the application of learning cycles, TADIR (Translation, Analysis, Design, Implementation and Revision, was used. An array of different ways to promote activities, tasks, results, and products generated by the project was established before, during and after the execution in an easy and complete manner. The population was also made aware of the advantages of agrometeorology and its importance for the agricultural development of the country. The results of this research can be made directly or indirectly available to the same organization or any others who wish to take the challenge of contributing

  8. Zebrafish: an animal model for research in veterinary medicine.

    Science.gov (United States)

    Nowik, N; Podlasz, P; Jakimiuk, A; Kasica, N; Sienkiewicz, W; Kaleczyc, J

    2015-01-01

    The zebrafish (Danio rerio) has become known as an excellent model organism for studies of vertebrate biology, vertebrate genetics, embryonal development, diseases and drug screening. Nevertheless, there is still lack of detailed reports about usage of the zebrafish as a model in veterinary medicine. Comparing to other vertebrates, they can lay hundreds of eggs at weekly intervals, externally fertilized zebrafish embryos are accessible to observation and manipulation at all stages of their development, which makes possible to simplify the research techniques such as fate mapping, fluorescent tracer time-lapse lineage analysis and single cell transplantation. Although zebrafish are only 2.5 cm long, they are easy to maintain. Intraperitoneal and intracerebroventricular injections, blood sampling and measurement of food intake are possible to be carry out in adult zebrafish. Danio rerio is a useful animal model for neurobiology, developmental biology, drug research, virology, microbiology and genetics. A lot of diseases, for which the zebrafish is a perfect model organism, affect aquatic animals. For a part of them, like those caused by Mycobacterium marinum or Pseudoloma neutrophila, Danio rerio is a natural host, but the zebrafish is also susceptible to the most of fish diseases including Itch, Spring viraemia of carp and Infectious spleen and kidney necrosis. The zebrafish is commonly used in research of bacterial virulence. The zebrafish embryo allows for rapid, non-invasive and real time analysis of bacterial infections in a vertebrate host. Plenty of common pathogens can be examined using zebrafish model: Streptococcus iniae, Vibrio anguillarum or Listeria monocytogenes. The steps are taken to use the zebrafish also in fungal research, especially that dealing with Candida albicans and Cryptococcus neoformans. Although, the zebrafish is used commonly as an animal model to study diseases caused by external agents, it is also useful in studies of metabolic

  9. RESPONSES OF QUINOA (CHENOPODIUM QUINOA WILLD. TO TWO CONDITIONS OF IRRIGATION IN COAST

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    La Rosa, R.

    2008-01-01

    Full Text Available 13 Artículo original Biologist (Lima. Vol. 6, Nº1, ene-jun 2008, 13-21 RESPUESTAS DE LA QUINUA (CHENOPODIUM QUINOA WILLD. A DOS CONDICIONES DE RIEGO EN COSTA RESPONSES OF QUINOA (CHENOPODIUM QUINOA WILLD. TO TWO CONDITIONS OF IRRIGATION IN COAST Rafael La Rosa1, Yesenia Macabilca2, Augusto Mendoza3 & Ana Gutiérrez 3 1 Laboratorio de Ecofisiología Vegetal; 2 Laboratorio de Bioquímica y Biología Molecular3; Centro de Investigaciones Agroecológicas Oquendo. Facultad de Ciencias Naturales y Matemática. Universidad Nacional Federico Villarreal. Calle San Marcos 351, Pueblo Libre, Lima – Perú. Teléfono 2193600 anexo 8373. Correo electrónico: rafolarosa@yahoo.es ABSTRACT Responses of Chenopodium quinoa Willd “quinoa” under two coast conditions of irrigation and its effects in production and quality of proteins were evaluated. In the current research we used seeds of variety “huancayo”, from Experimental Station “Santa Ana” of INIA (Institute Nacional de Investigaciones Agropecuarias– Huancayo. Irrigation was for drip, obtaining two treatments, i irrigation with 3 m3 of water, weekly, and ii without irrigation, while evaluations were done. Soil humidity, transpiration, relative water content (RWC, anatomy of mesophyll of leaves, quantification of proteins and starch, acid phosphatase activity and amilolytic activity were evaluated. Soil humidity is significant different after a month of irrigation. Transpiration was related with wind strength; hence this is not a good way to measure drought stress. RWC was similar in both treatments, it means that decrease in soil water not affect water level in leaves. Mesophylls were very similar in both conditions. Dry matter in vegetative period show no significant differences, but there were a tendency to accumulate more assimilates in irrigated plants. Therefore there were no difference in photosynthetic activity, so seeds received same quantity of assimilates; this fact means seeds

  10. Molecular, morphological and pathogenic characterization of six strains of Metarhizium spp. (Deuteromycotina: Hyphomycetes for the control of Aegorhinus superciliosus (Coleoptera: Curculionidae

    Directory of Open Access Journals (Sweden)

    María Sepúlveda

    2016-03-01

    Full Text Available Aegorhinus superciliosus is an important pest on blueberry (Vaccinium corymbosum L. and other fruit trees. The use of entomopathogenic fungi as Metarhizium spp. has been evaluated for the control of this insect, but variability has been observed among different strains. The aim of this study was to characterize six promising strains of Metarhizium spp. for the control of A. superciliosus. The studied strains were QuM173c, Qu-M363, Qu-M171a, Qu-M156a, Qu-M421, and Qu-M430, all of which belonged to the Chilean Collection of Microbial Genetic Resources (ChCMGR of the Institute de Investigaciones Agropecuarias (INIA, Chile. Molecular characterization was made by sequencing the ITS region (Internal Transcribed Spacers, ITS-5.8S rDNA. The morphology of conidia was evaluated through scanning electron microscopy and radial colony growth was evaluated in potato dextrose agar (PDA, Sabouraud dextrose agar (SDA, agar enriched with larvae of Galleria mellonella (Lepidoptera: Pyralidae (GA, and agar enriched with adults of A. superciliosus (AA. Pathogenicity was studied based on mortality of adults of A. superciliosus inoculated with conidia. Sequencing of the ITS-5.8S rDNA region indicates that the strains belong to the clade of M. anisopliae var. anisopliae, except for Qu-M171a, which was identified as M. anisopliae var. lepidiotum. Conidia average length for the six strains was 5.09 pm and average conidia width was 1.92 pm. Radial colony growth differences were observed between strains (p < 0.01 and between different growth media (p < 0.01. The strains exhibited the highest colony growth in the GA medium, while in the AA medium they showed the lowest (p < 0.01. Pathogenicity tests show that Qu-M430 reached a 90% mortality rate (p < 0.01. Results show that there is variability between the studied strains, which is expressed in their morphology, molecular characteristics and pathogenicity towards A. superciliosus.

  11. Zebrafish: an animal model for research in veterinary medicine.

    Science.gov (United States)

    Nowik, N; Podlasz, P; Jakimiuk, A; Kasica, N; Sienkiewicz, W; Kaleczyc, J

    2015-01-01

    The zebrafish (Danio rerio) has become known as an excellent model organism for studies of vertebrate biology, vertebrate genetics, embryonal development, diseases and drug screening. Nevertheless, there is still lack of detailed reports about usage of the zebrafish as a model in veterinary medicine. Comparing to other vertebrates, they can lay hundreds of eggs at weekly intervals, externally fertilized zebrafish embryos are accessible to observation and manipulation at all stages of their development, which makes possible to simplify the research techniques such as fate mapping, fluorescent tracer time-lapse lineage analysis and single cell transplantation. Although zebrafish are only 2.5 cm long, they are easy to maintain. Intraperitoneal and intracerebroventricular injections, blood sampling and measurement of food intake are possible to be carry out in adult zebrafish. Danio rerio is a useful animal model for neurobiology, developmental biology, drug research, virology, microbiology and genetics. A lot of diseases, for which the zebrafish is a perfect model organism, affect aquatic animals. For a part of them, like those caused by Mycobacterium marinum or Pseudoloma neutrophila, Danio rerio is a natural host, but the zebrafish is also susceptible to the most of fish diseases including Itch, Spring viraemia of carp and Infectious spleen and kidney necrosis. The zebrafish is commonly used in research of bacterial virulence. The zebrafish embryo allows for rapid, non-invasive and real time analysis of bacterial infections in a vertebrate host. Plenty of common pathogens can be examined using zebrafish model: Streptococcus iniae, Vibrio anguillarum or Listeria monocytogenes. The steps are taken to use the zebrafish also in fungal research, especially that dealing with Candida albicans and Cryptococcus neoformans. Although, the zebrafish is used commonly as an animal model to study diseases caused by external agents, it is also useful in studies of metabolic

  12. Molecular characterization and comparative expression analysis of two teleostean pro-inflammatory cytokines, IL-1β and IL-8, from Sebastes schlegeli.

    Science.gov (United States)

    Herath, H M L P B; Elvitigala, Don Anushka Sandaruwan; Godahewa, G I; Umasuthan, Navaneethaiyer; Whang, Ilson; Noh, Jae Koo; Lee, Jehee

    2016-01-10

    Interleukin 1β (IL-1β) and interleukin 8 (IL-8) are two major pro-inflammatory cytokines which play a central role in initiation of inflammatory responses against bacterial- and viral-infections. IL-1β is a member of the interleukin 1 family proteins and IL-8 is classified as a CXC-chemokine. In the current study, putative IL-1β and IL-8 counterparts were identified from a black rockfish transcriptomic database and designated as RfIL-1β and RfIL-8. The RfIL-1β cDNA sequence consists of 1140 nucleotides with a 759bp open reading frame (ORF) which encodes a 252 amino acid (aa) protein, whereas the RfIL-8 cDNA sequence (898bp) harbors a 300bp ORF encoding a 99 aa protein. Furthermore, the RfIL-1β aa sequence contains an IL-1 super family-like domain and an N-terminal IL-1 super family propeptide, while the amino acid sequence of RfIL-8 consists of a typical chemokine-CXC domain. Analysis of sequenced BAC clones containing RfIL-1β and RfIL-8 showed each gene to contain 4 exons interrupted by 3 introns. Pairwise comparison and phylogeny analysis of these cytokine sequences clearly revealed their closer relationship with other corresponding members of teleosts compared to birds and mammals. Constitutive differences in RfIL-1β and RfIL-8 mRNA expression were detected in a tissue-specific manner with the highest expression of each mRNA in spleen tissue. Two immune challenge experiments were conducted with Streptococcus iniae and polyinosinic:polycytidylic acid (poly I:C; a viral double stranded RNA mimic), and transcripts were quantified in spleen and peripheral blood cells. Significantly increased RfIL-1β and RfIL8 transcript levels were detected with almost similar profile patterns, further suggesting a putative involvement of these pro-inflammatory cytokines in the rockfish immunity. PMID:26449313

  13. Potassium and Phosphorus in Muscat Rosada Grape Yield in Elqui Valley Soil Fósforo y Potasio en la Producción de Vid Moscatel Rosada, en Suelo del Valle de Elqui

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    Carlos Sierra B

    2008-09-01

    Full Text Available The effects of P and K on grapevines (Vitis vinifera L. var. Muscat Rosada were evaluated four years, considering the same levels of N. The experiment was conducted at the Vicuña Experimental Station (30° S; 70°44´ W of the Instituto de Investigaciones Agropecuarias (INIA. The soil is alluvial antropic miscellaneous (Entisols. Three fertilization treatments were established: 1 N 160 + P2O5 200 + K2O 300; 2 N 160 + P2O5 0 + K2O 300; and 3 N 160 + P2O5 200 + K2O 0. At the beginning of the experiment, grape vines received all the phosphate fertilizers, 200 kg ha-1 of P2O5 as triple superphosphate in Treatments 1 and 3. Nitrogen was applied as urea for four years. The applications were initiated when the vine buds measured 20 cm. The K was applied to the soils of Treatments 1 and 2, at a dosage of 200 kg ha-1 K2O, using potassium sulphate. Between berry set and 30 d before harvest, 100 kg ha-1 K2O were applied as potassium sulphate by fertigation. Significant effects of the P fertilization were observed by the second year, with increased cluster numbers per plant. K also increased grape fruit yield by the third year. This response was obtained with less than 5 mg kg-1 of P available in the soil and less than 145 mg kg-1 of exchangeable K in the soil. The initially low content of available K and P suggests a high probability of response to the application of both elements.En el Campo Experimental del Instituto de Investigaciones Agropecuarias (INIA, ubicado en la localidad de Vicuña (30° S; 70°44´ O, se evaluó durante cuatro años el efecto de la aplicación de P y K, considerando un mismo nivel de aplicación de N en vid (Vitis vinifera L. var. Moscatel Rosada. El suelo corresponde al tipo misceláneo antrópico coluvial (Entisols. Se establecieron tres tratamientos de fertilización: 1 N 160 + P2O5 200 + K2O 300; 2 N 160 + P2O5 0 + K2O 300; y 3 N 160 + P2O5 200 + K(20 0. El parrón recibió toda la fertilización fosfatada aplicada al

  14. Melhoramento do trigo: XXV. Avaliação de genótipos oriundos de populações híbridas introduzidas de Oregon (EUA no Estado de São Paulo Wheat breeding: XXV. Evaluation of genotypes obtained from hybrid populations introduced from Oregon, U.S.A.,in the State of São Paulo, Brazil

    Directory of Open Access Journals (Sweden)

    Carlos Eduardo de Oliveira Camargo

    1991-01-01

    Full Text Available Foram comparadas entre si vinte e duas linhagens e três cultivares (BH-1146, IAC-18 e Alondra-S-46 em seis ensaios, instalados nas Estações Experimentais de Tatuí (1985-87 e Mococa (1984, no Centro Experimental de Campinas (1985 e na Fazenda Nossa Senhora da Penha (1986, município de Florínea, em condições de irrigação por aspersão, analisando-se os seguintes parâmetros: rendimento de grãos, características agronômicas e resistência às doenças. Em casa de vegetação, efetuaram-se estudos de resistência às misturas de raças prevalecentes dos agentes causais da ferrugem-do-colmo e da-folha e, em condições de laboratório, estudos da tolerância ao alumínio, em soluções nutritivas. Em solos corrigidos de Tatuí, a linhagem 1 (Novi Sad 738/Bluejay, sensível à toxicidade de Al3+, de porte baixo, ciclo precoce, destacou-se quanto à produção de grãos. Em solo ácido, de Mococa, os cultivares BH-1146 e IAC-18, tolerantes à toxicidade de Al3+, de porte alto e ciclo precoce, foram os mais produtivos. As linhagens 1,3 (Yaktana 54/Norin 10 - Brevor//Narino 59/3/Hyslop/4/CIANO/Gallo e 19 (Capitole/Bluetit e os cultivares BH-1146 e IAC-18 destacaram-se quanto à produção de grãos, considerando-se a média dos seis ensaios. As linhagens 2 (Leonardo 23/Bluejay e 10 (Backa/Alondra foram resistentes às duas misturas de raças testadas da ferrugem-do-colmo e às três misturas de raças da ferrugem-da-folha, em estádio de plântula, confirmando esta resistência em condições de infecção natural no estádio de planta adulta. A linhagem 2 mostrou-se moderadamente resistente ao oídio. As linhagens 11 (Backa/Alondra, 15 e 21 (Capitole/Bluetit, 16 (Sava/4/Tezanos Pintos Precoz//IRN 46/ClANO/3/Protor e 17 (Vogel Selection 29/Vogel Selection 59-8881//INIA/CaprocK/3/Cuckoo foram as mais tolerantes à toxicidade de Al3+, porém num grau menor do que os exibidos pelos cultivares BH-1146 e lAC-18.Twenty two inbred lines and three

  15. Water requirements for wheat and maize under climate change in North Nile Delta

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    Samiha Ouda

    2015-03-01

    -M (Penman Monteith; RMSE (root mean square error; WHC (water holding capacity. Citation: Ouda, S.; Noreldin, T.; Abd El-Latif, K. (2015. Water requirements for wheat and maize under climate change in North Nile Delta. Spanish Journal of Agricultural Research, Volume 13, Issue 1, e03-001, 10 pages. http://dx.doi.org/10.5424/sjar/2015131-6412. Received: 15 Jun 2014. Accepted: 02 Dec 2014. http://dx.doi.org/10.5424/sjar/2015131-6412 Copyright © 2015 INIA. This is an open access article distributed under the Creative Commons Attribution License (CC by 3.0, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Funding: The author(s received no specific funding for this work. Competing interests: The authors have declared that no competing interests exist. Correspondence should be addressed to Samiha Ouda: samihaouda@yahoo.com

  16. Analysis of the degradation of biodegradable mulches in a pepper crop under organic management

    Science.gov (United States)

    Moreno, Carmen; González, Sara; Villena, Jaime; Meco, Ramón; María Moreno, Marta

    2016-04-01

    Mimgreen® paper remained practically intact or with little damaged until the end of the crop season, while biopolymers suffered further deterioration, especially Bioflex®. ii) The buried part of the Mimgreen® paper underwent a rapid and complete degradation, while biopolymers presented a variable behavior, from practically intact, similar to polyethylene (Ecovio®), to an important decrease of the soil covered (Mater-Bi® and Sphere 4®). iii) A visual understatement of the functionality of the material (subjective scales) was observed when compared with the numerical value of the percentage of the soil covered by the mulch. Acknowledgements: the research was funded by Project RTA2011-00104-C04-03 from the INIA (Spanish Ministry of Economy and Competitiveness).

  17. VACCINATION OF HYBRID STRIPED BASS: GROWTH, IMMUNE REACTION AND GENE EXPRESSION

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    Mark Westerman

    2012-10-01

    Full Text Available Hybrid striped bass (42.6+4.9 g wet wt; 139.3+6.1 mm length, were randomly stocked into one of 6 tanks (n=6 tank-1 of a custom designed, recirculating life support system (RLSS. Water quality was as follows: DO2 (6.50.6 mg l-1, pH (7.70.5, TAN (0.06-1.31 mg l-1, nitrite (0.06-0.60 mg l-1 and nitrate (2.0-32.1 mg l-1, salinity 5 ppt, temperature 28±1 oC. A 12:12 photophase:scotophase was used, with a 30 min. dusk-dawn dimming of lights. Fish were fed at 4% body wt d-1 as two separate feedings (08.00 and 16.00 h. Dietary crude protein and lipid levels were 40% and 10% respectively. Tanks were randomly paired and fish either left untreated, vaccinated, or sham injected. The vaccine employed was an experimental formalin killed Streptococcus iniae oil-in-water adjuvanted bacterin. Fish were weighed and measured bimonthly for 8 wk with group weights being employed to adjust feeding rates. At trial termination, all animals were weighed and measured, and their condition factor (CF and feed conversion ratios (FCR calculated. Visceral somatic (VSI and hepatosomatic (HSI indices and intraperitoneal fat (IPF and muscle ratios (MR were also assessed. Blood was taken from anaesthetized fish and hematocrit recorded. Blood was allowed to clot overnight at 5 oC after which serum protein levels were recorded and lysozyme activity measured. Livers were prepared for microarray evaluation using standard techniques using a Danio rerio genechip to assess global changes in gene expression. No differences were observed with respect to final weights, lengths, CF, FCR, or HSI although differences (P < 0.001 were determined for VSI, which was higher in control animals. Packed cell volume and serum protein levels were similar across groups (P > 0.05. Time-course of changes in serum lysozyme activity described an initial reduction in lysozyme activity followed by a rebounding in activity which peaked 25 days post-treatments. Evaluation of lysozyme activity among time

  18. 罗非鱼源无乳链球菌双重PCR快速检测方法的建立%Development of double PCR for rapid detection of Streptococcus agalactiae isolated from tilapia

    Institute of Scientific and Technical Information of China (English)

    王均; 汪开毓; 肖丹; 黄锦炉; 付希; 王浩丞; 陈德芳; 耿毅; 阳涛

    2011-01-01

    A double PCR method for rapid detection of Streptococcus agalactiae was established. Two pairs of specific primers based on cpsF gene of tilapia S. agalactiae capsular polysaccharides and cfb gene of factor CAMP published in GenBank were designed respectively to perform PCR with optimization of reaction system and reaction condition. In results,two specific bands,cpsF and cfb,were only detected in the S. agalactiae isolates, and none was amplified from Streptococcus iniae ,Staphylococcus aureus , Edwardsiella ictaluri , Aeromonas hydrophila , Aeromonas salmonicida , Aeromonas caviae or Stenotrophomonas maltophilia. The detection limit of the S. agalactiae genomic DNA was 7. 632 × 10-2 ng/μL. The double PCR detection and the bacterial isolation and identification were performed with bacteria DNA in the tissues of liver and kidney of 30 tilapias challenged with S. agalactiae and the positive results by the double PCR were consistent with the positive results by the bacterial isolation and identification. In conclusion,the established double PCR in the present study was specific,sensitive and rapid for the epidemiological surveillance of S. agalactiae infection in tilapia and other fish.%根据GenBank中罗非鱼源无乳链球菌荚膜多糖的cpsF基因和CAMP因子的cfb基因序列,设计2对特异性引物,通过对反应体系和反应条件优化,并进行特异性试验、敏感性试验及人工感染罗非鱼组织样品检测,建立了快速检测无乳链球菌的双重PCR方法.结果显示,仅无乳链球菌能扩增出cpsF和cfb两条特异性条带,而海豚链球菌、金黄色葡萄球菌、鲴爱德华氏菌、嗜水气单胞菌、杀鲑气单胞菌、豚鼠气单胞菌、嗜麦芽寡养单胞菌无条带检出;经敏感性试验,最小模板检出量为7.632×10-2ng/μL;同时对30尾人工感染无乳链球菌的罗非鱼肝和肾组织中细菌DNA进行双重PCR检测和细菌分离鉴定,检出的阳性结果一致.证实,所建立的方法

  19. Transcriptional regulation of multi-drug tolerance and antibiotic-induced responses by the histone-like protein Lsr2 in M. tuberculosis.

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    Roberto Colangeli

    2007-06-01

    Full Text Available Multi-drug tolerance is a key phenotypic property that complicates the sterilization of mammals infected with Mycobacterium tuberculosis. Previous studies have established that iniBAC, an operon that confers multi-drug tolerance to M. bovis BCG through an associated pump-like activity, is induced by the antibiotics isoniazid (INH and ethambutol (EMB. An improved understanding of the functional role of antibiotic-induced genes and the regulation of drug tolerance may be gained by studying the factors that regulate antibiotic-mediated gene expression. An M. smegmatis strain containing a lacZ gene fused to the promoter of M. tuberculosis iniBAC (PiniBAC was subjected to transposon mutagenesis. Mutants with constitutive expression and increased EMB-mediated induction of PiniBAC::lacZ mapped to the lsr2 gene (MSMEG6065, a small basic protein of unknown function that is highly conserved among mycobacteria. These mutants had a marked change in colony morphology and generated a new polar lipid. Complementation with multi-copy M. tuberculosis lsr2 (Rv3597c returned PiniBAC expression to baseline, reversed the observed morphological and lipid changes, and repressed PiniBAC induction by EMB to below that of the control M. smegmatis strain. Microarray analysis of an lsr2 knockout confirmed upregulation of M. smegmatis iniA and demonstrated upregulation of genes involved in cell wall and metabolic functions. Fully 121 of 584 genes induced by EMB treatment in wild-type M. smegmatis were upregulated ("hyperinduced" to even higher levels by EMB in the M. smegmatis lsr2 knockout. The most highly upregulated genes and gene clusters had adenine-thymine (AT-rich 5-prime untranslated regions. In M. tuberculosis, overexpression of lsr2 repressed INH-mediated induction of all three iniBAC genes, as well as another annotated pump, efpA. The low molecular weight and basic properties of Lsr2 (pI 10.69 suggested that it was a histone-like protein, although it did not

  20. The Selection and Determinaton of the Pest-resistant Character in    Transgenic Regenerative Rice Plants and Their Descendances%水稻转基因再生植株及其后代抗虫性筛选测定研究

    Institute of Scientific and Technical Information of China (English)

    冉学忠; 王立敏; 孙伯铮; 陈方江; 刘春颖

    2001-01-01

    Using gene gun transformation system, we transfered CpTI (Cowpea Trypsin Inhibitor)gene to rice callus tissues;through anti-biotic selection.The transgenic regenerative rice plants were gotten. Indoor feeding corn borer(Ostrinia nubilalis) by these transgenic rice regenaration plants and their descendance’s tiller stem;The corn borer mortality and the worm length were compared to determinate transgenic regenerative rice’s pest resistance. From 1997 to 1999,5 indoor tests had been taken on three generations. 15 better pest resistance plants were selected from 121 plants in F0 generation, 7 strains 40 better single plants from 15 strains in F1 generation,and 37 better plants from 14 strains in F2 generation. We did molecular test(PCR) to 19 plants from the 37 better plants,and asserted that 5 material are homozygous plants, the other 14 material have segregation. All of these stated that the anti-pest gene in transgenic regenerative rice can express and is heritable%采用基因枪转化系统,将CpTI(豇豆胰蛋白酶抑制剂)抗虫基因转入水稻愈伤组织中,经过抗生素筛选得到了再生植株。我们用这些转基因水稻再生植株及其后代的分蘖茎段对玉米螟(Ostrinia nubilalis)进行室内喂养,通过比较虫体死亡率及虫体生长长度,测定转基因水稻再生植株的抗虫性。1997~1999年共进行3个世代5次的室内测定。从121株F0代转基因植株中选出抗虫性较好的植株15株。从F1代的15个株系中,又筛选出7个株系40个单株;在F2代的14个株系中共选出37株抗虫效果表现较好的植株。对37株中的19株进行了分子检测(PCR扩增),从中检测出5份材料为纯合体,14份材料有分离现象。研究结果说明:该室内喂养玉米螟并进行抗虫测定的方法是可行的;抗虫基因在转基因水稻再生植株中已经获得表达并且可以遗传

  1. Rapid Detection of Stretococcus agalactiae Isolated from Tilapia with Double PCR%双重 PC R检测罗非鱼源无乳链球菌方法的建立

    Institute of Scientific and Technical Information of China (English)

    樊海平; 吴斌; 张新艳; 邓志武; 郑磊; 钟全福; 曾占壮

    2014-01-01

    Two pairs of specific primers were designed and synthesized according to the 16S rRNA and Surface immunogenic protein (sip) gene sequence of tilapia Stretococcus agalactiae ,and a double-PCR method for rapid detection of Streptococcus agalactiae isolated from tilapia was established through optimization of reaction system and reaction condition ,by which two specific fragments for S .agalactiae of 1 305 bp and 121 bp were producted . Specificity test showed that two specific bands ,16S rRNA and sip ,could only be detected in the S . agalactiae , and none was amplified from Streptococcus inia .The minimum detectable quantity of the S . agalactiae strains 070717LL was 1.05 × 102 CFU . In the tissues from spleen , brain and kidney of tilapias affected by the S . agalactiae strain 070717LL the strains DNA were detected , especially in kidney and spleen tissues . In conclusion ,this double-PCR method was specific ,sensitive rapid ,and applicable for epidemiological investigation of S . agalactiae in tilapia .%根据罗非鱼源无乳链球菌16S rRNA 基因和sip基因序列,设计、合成2对特异性引物,通过对反应体系和反应条件优化,建立快速检测无乳链球菌的双重PCR方法。该方法扩增无乳链球菌可获得1305 bp和121 bp 2个特异性片段;对6株链球菌属的菌株进行特异性分析,仅无乳链球菌能扩增出16S rRNA 基因和sip基因2条特异性条带,而海豚链球菌无条带检出;经灵敏度试验,可检测到无乳链球菌菌株070717LL的最小量为1.05×102 CFU ;同时,双重PCR可以检测出无乳链球菌菌株070717LL人工感染的罗非鱼脾、脑、肾组织中细菌DNA ,特别是脾脏和肾脏组织的样品检测效果好。结果证明所建立的方法具有快速、特异性强、灵敏度高等优点,适用于对罗非鱼源无乳链球菌病的流行病学调查。

  2. Construction and Identification of Full-length cDNA Library of the Peripheral Blood Leucocytes in Oreochromis niloticus after Vaccination%尼罗罗非鱼免疫后外周血白细胞全长cDNA文库的构建及鉴定

    Institute of Scientific and Technical Information of China (English)

    陈明; 梁万文; 李超; 王瑞; 李莉萍; 甘西; 余晓丽; 雷爱莹; 黄婷; 陈福艳

    2011-01-01

    为大规模快速克隆罗非鱼细胞免疫功能基因,采用SMART技术,构建了尼罗罗非鱼链球菌疫苗免疫后外周血白细胞全长cDNA文库.提取免疫后第3、5和7天外周血白细胞总RNA,用Powerscrip TM反转录酶逆转录合成第一链cDNA,LD-PCR扩增获得双链cDNA,经sfiⅠ酶切和CHROMA SPIN-400TM柱分级分离,收集500 bp以上的片段重组于改造的pBluescriptⅡ SK载体并转化大肠杆菌DH5α,测定文库滴度、重组率及库容量.结果表明,构建的cDNA文库原始库容量1.021×106克隆,文库滴度1.078×106pfu/mL,重组率94.71%,插入片段大小0.75-3.0 kb,平均长度约为1.5 kb.随机对24个克隆测序所获得的18条Contigs进行BLASTx分析,发现有15条Contigs有相关同源信忠,其中10条为全长cDNA,全长串为66.7%.说明所构建文库的各项指标均达到要求,可为筛选罗非鱼免疫功能相关基因和进一步研究基因的结构和功能奠定基础.%In order to rapidly clone the cellular immune function genes of the Tiplia on a large scale, a full-length cDNA library of the peripheral blood leucocytes from the Oreochromis niloticus thud vaccinated of S. iniae vaccine by i. p was constructed by using SMART ( switching mechanism at 5′ end of RNA transcript) techniques. The total RNA was extracted from peripheral blood teucocytes after vaccinated 3 , 5 , and 7 days. The PowerscriptTM reverse transcriptase was used to synthesize and anchor the first-strand cDNA, and the long distance PCR ( LD-PCR) method was used to amplify double-strand cDNA based on the SMART techniquea for construction of a full-length cDNA library. The PCR products were digested by proteinase K. After digestion with Sfi Ⅰ and size fractionation using CHROMA SPIN-400TM columns, cDNAa ( > 500 bp ) were ligated to the Sfi Ⅰ digested , dephosphorylated pBluescript Ⅱ SK vector. The ligation mixture was transformed into E. coil DH5a. The recmnbinant vectors were titered and the recombinant rate

  3. 应用牙科分光光度比色仪对钴铬合金基底表面4种全瓷冠色度学特征的研究%The study of the colorimetric characteristics of the cobalt-chrome alloys abutments covered by four different all-ceramic crowns by using dental spectrophotometer

    Institute of Scientific and Technical Information of China (English)

    陈奕帆; 刘红春; 孟玉坤; 巢永烈; 刘长虹

    2015-01-01

    ObjectiveThis study aims to evaluate the optical data of the different sites of the cobalt-chrome (Co-Cr) alloy abutments covered by four different all-ceramic crowns and the color difference between the crowns and target tab using a digital dental spectrophotometer. Methods   Ten Co-Cr alloy abutments were made and tried in four different groups of all-ceramic crowns, namely, Procera aluminia, Procera zirconia, Lava zirconia (Lava-Zir), and IPS E.max glass-ceramic lithium disilicate-reinforced monolithic. The color data of the cervical, body, and incisal sites of the samples were recorded and analyzed by dental spectrophotometer. The CIE L*, a*, b* values were again measured after veneering. The color difference between the abutments covered by all-ceramic crowns and A2 dentine shade tab was evaluated. Results   The L* and b* values of the abut-ments can be increased by all of the four groups of all-ceramic copings, but a* values were decreased in most groups. A statistical difference was observed among four groups. After being veneered, the L* values of all the copings declined slightly, and the values of a*, b* increased significantly. When compared with A2 dentine shade tab, the ΔΕ of the crowns was below 4. Conclusion   Four ceramic copings were demonstrated to promote the lightness and hue of the alloy abutments effec-tively. Though the colorimetric baseline of these copings was uneven, veneer porcelain can efficiently decrease the color difference between the samples and thee target.%目的:采用牙科分光光度比色仪

  4. Distribution and Antimicrobial Susceptibility Analysis of Common Pathogenic Bacteria Isolated from Cold-water Fish in Three North Areas of China%三北地区冷水鱼常见病原菌的分布及药敏试验

    Institute of Scientific and Technical Information of China (English)

    连浩淼; 李绍戊; 张辉; 卢彤岩

    2015-01-01

    because of high density,bad water quality,poor quality of feed and so on,which could also indirectly affect people’ s health. At present,control of bacterial diseases still depends on antibiotics in China,but the abuse of antibiotics will cause drug residues and multi⁃drug resistance of bacteria,so it is necessary for us to study the characteristics of pathogenic bacteria existing in cold⁃water fish aquaculture. In this study,common pathogenic bacteria were isolated and identified from farm⁃raised rainbow trout,Atlantic salmon,silver salmon and Siberia sturgeon in the three north areas of China during the period from May to August in 2013.Based on the epidemiology investigation,different kinds of diseases occurred in fish with different clinical signs:gill⁃rot, haemorrhage in diseased rainbow trout from Qinghai Province;exophthalmosis,darkening in color,saprolegnia⁃sis and bleeding spots in internal organs in diseased silver salmon from Liaoning Province;enteritis,gill⁃rot in Atlantic salmon from Heilongjiang Province and haemorrhage on body surface,mouth irritation,etc. in Siberia sturgeon from Beijing City.Samples were collected and streaked on the plate,and isolated bacteria were inocu⁃lated under aseptic conditions. The results indicated that 12 strains of Aeromonas salmonicida and 1 strain of Acinetobacter were isolated from diseased silver salmon cultured in Liaoning;14 strains of Aeromonas salmonici⁃da,6 strains of Aeromonas sobria,2 strains of Aeromonas hydrophila,6 strains of Acinetobacter,2 strains of Fla⁃vobacterium were isolated from diseased Atlantic salmon cultured in Mudanjiang;9 strains of Aeromonas sobria, 6 strains of Aeromonas veronii,6 strains of Acinetobacter,2 strains of Flavobacterium,2 strains of Yersinia ruckeri were isolated from diseased rainbow trout cultured in Qinghai;3 strains of Streptococcus iniae,3 strains of Acine⁃tobacter 10 strains of Streptococcus dysgalactiae,2 strains of Aeromonas veronii,2 strains of Aeromonas

  5. Oviposition Preferences of Major Lepidoptera Pest of Pinus kesiya var.langbianensis%思茅松主要鳞翅目害虫的产卵选择性

    Institute of Scientific and Technical Information of China (English)

    马惠芬; 刘凌; 闫争亮; 刘云彩

    2013-01-01

    运用同时蒸馏萃取( SDE)和气相色谱-质谱联用技术( GC-MS)比较了思茅松( Pni usk esiyavar 。 langbianensis)、阿丁枫( Altingia chinensis)和西南桦( Bteu la alnoides)的树叶挥发性物质的组成和含量,并对松实小卷蛾( Rte inia crstata)、微红梢斑螟( Dioryctria rubella)、思茅松毛虫( Dendrolimus kikuchii)在3种树上的产卵选择性进行了试验。 GC-MS分析结果表明:思茅松针叶的挥发性物质以α-蒎烯为主,也含有较多的β-蒎烯、莰烯、石竹烯、月桂烯和β-水芹烯等萜烯类化合物;阿丁枫树叶的挥发物以( E)-石竹烯、异石竹烯、α-依兰油烯、愈创烷-1(5),11-二烯及双环大根香叶烯为主;而西南桦树叶挥发物中,除含有α-蒎烯等萜烯类化合物外,主要以2-己烯醛、2-己烯-1-醇、( Z)-3-己烯-1-醇、( Z)-2-己烯-1-醇等绿叶气味物质为主。产卵选择性试验表明:松实小卷蛾、微红梢斑螟、思茅松毛虫偏好选择思茅松针叶为其产卵场所,而将思茅松喷施阿丁枫和西南桦树叶提取物后,针叶上的产卵量大大减少。说明植物挥发物在松实小卷蛾、微红梢斑螟、思茅松毛虫等鳞翅目松树害虫的产卵寄主选择中起关键作用,松树挥发物提供了寄主对雌虫产卵适宜性的化学信息,而阔叶树挥发物则对其产卵具有驱避作用。%By simultaneous distillation extraction (SDE) and gas chromatography-mass spectrometry (GC-MS), we compared the leaf volatile compositions of Pinus kesiya var.l angbianensis, Altingia chinensis, Betula alnoides and we tested oviposi-tion preferences of Re tiin a csr tata, Dioryctria rubella and Dendrolimus kikuchi .In volatile of Pinus kesiya var.langbianen-sis,α-pinene is the dominant component.β-pinene, camphene,caryophyllene , myrcene and βp-hellandrene are also the maincomponents .In volatile ofA ltingi a chinensis, (E

  6. 三北地区冷水鱼常见病原菌的分布及药敏试验%Distribution and Antimicrobial Susceptibility Analysis of Common Pathogenic Bacteria Isolated from Cold-water Fish in Three North Areas of China

    Institute of Scientific and Technical Information of China (English)

    连浩淼; 李绍戊; 张辉; 卢彤岩

    2015-01-01

    because of high density,bad water quality,poor quality of feed and so on,which could also indirectly affect people’ s health. At present,control of bacterial diseases still depends on antibiotics in China,but the abuse of antibiotics will cause drug residues and multi⁃drug resistance of bacteria,so it is necessary for us to study the characteristics of pathogenic bacteria existing in cold⁃water fish aquaculture. In this study,common pathogenic bacteria were isolated and identified from farm⁃raised rainbow trout,Atlantic salmon,silver salmon and Siberia sturgeon in the three north areas of China during the period from May to August in 2013.Based on the epidemiology investigation,different kinds of diseases occurred in fish with different clinical signs:gill⁃rot, haemorrhage in diseased rainbow trout from Qinghai Province;exophthalmosis,darkening in color,saprolegnia⁃sis and bleeding spots in internal organs in diseased silver salmon from Liaoning Province;enteritis,gill⁃rot in Atlantic salmon from Heilongjiang Province and haemorrhage on body surface,mouth irritation,etc. in Siberia sturgeon from Beijing City.Samples were collected and streaked on the plate,and isolated bacteria were inocu⁃lated under aseptic conditions. The results indicated that 12 strains of Aeromonas salmonicida and 1 strain of Acinetobacter were isolated from diseased silver salmon cultured in Liaoning;14 strains of Aeromonas salmonici⁃da,6 strains of Aeromonas sobria,2 strains of Aeromonas hydrophila,6 strains of Acinetobacter,2 strains of Fla⁃vobacterium were isolated from diseased Atlantic salmon cultured in Mudanjiang;9 strains of Aeromonas sobria, 6 strains of Aeromonas veronii,6 strains of Acinetobacter,2 strains of Flavobacterium,2 strains of Yersinia ruckeri were isolated from diseased rainbow trout cultured in Qinghai;3 strains of Streptococcus iniae,3 strains of Acine⁃tobacter 10 strains of Streptococcus dysgalactiae,2 strains of Aeromonas veronii,2 strains of Aeromonas