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  1. Automated design of genomic Southern blot probes

    Directory of Open Access Journals (Sweden)

    Komiyama Noboru H

    2010-01-01

    Full Text Available Abstract Background Sothern blotting is a DNA analysis technique that has found widespread application in molecular biology. It has been used for gene discovery and mapping and has diagnostic and forensic applications, including mutation detection in patient samples and DNA fingerprinting in criminal investigations. Southern blotting has been employed as the definitive method for detecting transgene integration, and successful homologous recombination in gene targeting experiments. The technique employs a labeled DNA probe to detect a specific DNA sequence in a complex DNA sample that has been separated by restriction-digest and gel electrophoresis. Critically for the technique to succeed the probe must be unique to the target locus so as not to cross-hybridize to other endogenous DNA within the sample. Investigators routinely employ a manual approach to probe design. A genome browser is used to extract DNA sequence from the locus of interest, which is searched against the target genome using a BLAST-like tool. Ideally a single perfect match is obtained to the target, with little cross-reactivity caused by homologous DNA sequence present in the genome and/or repetitive and low-complexity elements in the candidate probe. This is a labor intensive process often requiring several attempts to find a suitable probe for laboratory testing. Results We have written an informatic pipeline to automatically design genomic Sothern blot probes that specifically attempts to optimize the resultant probe, employing a brute-force strategy of generating many candidate probes of acceptable length in the user-specified design window, searching all against the target genome, then scoring and ranking the candidates by uniqueness and repetitive DNA element content. Using these in silico measures we can automatically design probes that we predict to perform as well, or better, than our previous manual designs, while considerably reducing design time. We went on to

  2. A simple DNA recombination screening method by RT-PCR as an alternative to Southern blot

    DEFF Research Database (Denmark)

    Albers, Eliene; Sbroggiò, Mauro; Martin Gonzalez, Javier

    2017-01-01

    The generation of genetically engineered mouse models (GEMMs), including knock-out (KO) and knock-in (KI) models, often requires genomic screening of many mouse ES cell (mESC) clones by Southern blot. The use of large targeting constructs facilitates the recombination of exogenous DNA in a specific...... that match exons flanking the targeting construct. The detection of the expected modification in this PCR product confirms the integration at the correct genomic location and shows that the mutant mRNA is expressed. This is a simple and sensitive strategy to screen locus-specific recombination of targeting...

  3. A simple DNA recombination screening method by RT-PCR as an alternative to Southern blot.

    Science.gov (United States)

    Albers, Eliene; Sbroggiò, Mauro; Martin-Gonzalez, Javier; Avram, Alexandra; Munk, Stephanie; Lopez-Contreras, Andres J

    2017-01-19

    The generation of genetically engineered mouse models (GEMMs), including knock-out (KO) and knock-in (KI) models, often requires genomic screening of many mouse ES cell (mESC) clones by Southern blot. The use of large targeting constructs facilitates the recombination of exogenous DNA in a specific genomic locus, but limits the detection of its correct genomic integration by standard PCR methods. Genomic Long Range PCR (LR-PCR), using primers adjacent to the homology arms, has been used as an alternative to radioactive-based Southern blot screenings. However, LR-PCRs are often difficult and render many false positive and false negative results. Here, we propose an alternative screening method based on the detection of a genetic modification at the mRNA level, which we successfully optimized in two mouse models. This screening method consists of a reverse-transcription PCR (RT-PCR) using primers that match exons flanking the targeting construct. The detection of the expected modification in this PCR product confirms the integration at the correct genomic location and shows that the mutant mRNA is expressed. This is a simple and sensitive strategy to screen locus-specific recombination of targeting constructs which can also be useful to screen KO and KI mutant mice or cell lines including those generated by CRISPR/Cas9.

  4. Fluorescent detection of Southern blots and PCR-based genetic typing tests

    Energy Technology Data Exchange (ETDEWEB)

    Mansfield, E.S.; Worley, J.M. [Molecular Dynamics, Inc., Sunnyvale, CA (United States); Zimmerman, P.A. [Laboratory of Parasitic Diseases, Bethesda, MD (United States)] [and others

    1994-09-01

    The Southern blot is used to study gene organization, to identify disease-causing genomic rearrangements, or for typing RFLP markers in forensic, paternity, or prenatal diagnostic testing. Fluorescence offers a much greater dynamic range and a more linear response than film used in radioactive or chemiluminescent detection of RFLPs. We therefore investigated using the Fluorimager{trademark} 575 (Molecular Dynamics, Inc.) for analyzing Southern blots. Using a single-locus probe to D2S44 (YNH24) (Promega Corp.), we detect as little as 100 ng (0.05 attomole) genomic DNA. The alkaline phosphatase-labeled probe is detected using AttoPhos (JBL Scientific), and the developed membrane is scanned with the Fluorimager. Biotinylated hybridization probes can also be developed using a streptavidin-alkaline phosphatase conjugate and AttoPhos. The instrument scan parameters can be adjusted to prevent overexposure and accompanying loss of resolution in images of blots, gels, or 96-well microplates. We have used these other sample formats in PCR-based genetic typing assays. We use FluorKit DQS (Molecular Dynamics) to accurately quantify PCR template DNA (1-500 ng) in 96-well microplates scanned using the same instrument. Mutation detection assays run include heteroduplex gels (5% polyacrylamide, 2.7 M urea), short tandem repeat (STR) markers, amplified fragment length polymorphisms (AmpFLP), competitive priming PCR, and allele-specific oligotyping. These assays are run using either 1- or 2-color labeling. We detect unlabeled PCR products, such as the AmpFLP marker D1S80 (Perkin-Elmer) by post-staining gels for 10 minutes with SYBR Green 1 (Molecular Probes) and scanning the wet gel. The Fluorimager scans a 20 x 25 cm sample within three minutes, allowing rapid optimization of fluorescent protocols and high sample throughput.

  5. Detection of Sleeping Beauty transposition in the genome of host cells by non-radioactive Southern blot analysis.

    Science.gov (United States)

    Aravalli, Rajagopal N; Park, Chang W; Steer, Clifford J

    2016-08-26

    The Sleeping Beauty transposon (SB-Tn) system is being used widely as a DNA vector for the delivery of therapeutic transgenes, as well as a tool for the insertional mutagenesis in animal models. In order to accurately assess the insertional potential and properties related to the integration of SB it is essential to determine the copy number of SB-Tn in the host genome. Recently developed SB100X transposase has demonstrated an integration rate that was much higher than the original SB10 and that of other versions of hyperactive SB transposases, such as HSB3 or HSB17. In this study, we have constructed a series of SB vectors carrying either a DsRed or a human β-globin transgene that was encompassed by cHS4 insulator elements, and containing the SB100X transposase gene outside the SB-Tn unit within the same vector in cis configuration. These SB-Tn constructs were introduced into the K-562 erythroid cell line, and their presence in the genomes of host cells was analyzed by Southern blot analysis using non-radioactive probes. Many copies of SB-Tn insertions were detected in host cells regardless of transgene sequences or the presence of cHS4 insulator elements. Interestingly, the size difference of 2.4 kb between insulated SB and non-insulated controls did not reflect the proportional difference in copy numbers of inserted SB-Tns. We then attempted methylation-sensitive Southern blots to assess the potential influence of cHS4 insulator elements on the epigenetic modification of SB-Tn. Our results indicated that SB100X was able to integrate at multiple sites with the number of SB-Tn copies larger than 6 kb in size. In addition, the non-radioactive Southern blot protocols developed here will be useful to detect integrated SB-Tn copies in any mammalian cell type.

  6. Assessment by Southern blot analysis of UV-induced damage and repair in human immunoglobulin genes.

    Science.gov (United States)

    Bianchi, M S; Bianchi, N O; de la Chapelle, A

    1990-09-01

    Irradiation of DNA with UV light induces pyrimidine dimers and (6-4) photoproducts. The presence of one of these photolesions in the restriction site of a given endonuclease inhibits DNA cleavage and induces the formation of fragments by incomplete DNA digestion which appear as additional, facultative bands in Southern hybridization autoradiograms. The number and size of these fragments show a positive correlation with the UV dose. The response to UV light of immunoglobulin light-chain constant kappa and heavy-chain constant mu genes was analyzed with 2 specific probes. Constant kappa and mu genes when irradiated as part of the chromatin of living lymphocytes showed a UV sensitivity similar to that of naked DNA. The same genes from granulocytes had 50-60 times lower UV sensitivity. When cells were allowed to repair photolesions for 24 h the facultative bands from granulocytes disappeared indicating that these cells were able to remove photolesions from constant kappa and mu genes. Facultative bands from lymphocytes showed a smaller decrease of density after 24 h repair. This suggests that lymphocytes are less efficient than granulocytes in removing UV damage from constant kappa and mu genes.

  7. Imprinting mutations in Angelman syndrome detected by Southern blotting using a probe containing exon {alpha} of SNRPN

    Energy Technology Data Exchange (ETDEWEB)

    Beuten, J.; Sutcliffe, J.S.; Nakao, M. [Baylor College of Medicine, Houston, TX (United States)] [and others

    1994-09-01

    Prader-Willi syndrome (PWS) and Angelman syndrome (AS) are associated with paternal and maternal deficiencies respectively, of gene expression within human chromosome 15q11-q13, and are caused by deletion, uniparental disomy (UPD), or other mutations. The SNRPN gene maps in this region, is paternally expressed, and is a candidate gene for PWS. Southern blotting using methylation-sensitive enzymes and a genomic DNA probe from the CpG island containing exon {alpha} of the SNRPN gene reveals methylation specific for the maternal allele. In cases of the usual deletions or UPD, the probe detects absence of an unmethylated allele in PWS and absence of a methylated allele in AS. We have analyzed 21 nondeletion/nonUPD AS patients with this probe and found evidence for an imprinting mutation (absence of a methylated allele) in 3 patients. Southern blotting with methylation-sensitive enzymes using the exon {alpha} probe, like use of the PW71 probe, should detect abnormalities in all known PWS cases and in 3 of the 4 forms of AS: deletion, UPD and imprinting mutations. This analysis provides a valuable diagnostic approach for PWS and AS. In efforts to localize the imprinting mutations in AS, one patient was found with failure to inherit a dinucleotide repeat polymorphism near probe 189-1 (D15S13). Analysis of this locus in AS families and CEPH families demonstrates a polymorphism that impairs amplification and a different polymorphism involving absence of hybridization to the 189-1 probe. The functional significance, if any, of deletion of the 189-1 region is unclear.

  8. A novel methylation PCR that offers standardized determination of FMR1 methylation and CGG repeat length without southern blot analysis.

    Science.gov (United States)

    Grasso, Marina; Boon, Elles M J; Filipovic-Sadic, Stela; van Bunderen, Patrick A; Gennaro, Elena; Cao, Ru; Latham, Gary J; Hadd, Andrew G; Coviello, Domenico A

    2014-01-01

    Fragile X syndrome and associated disorders are characterized by the number of CGG repeats and methylation status of the FMR1 gene for which Southern blot (SB) historically has been required for analysis. This study describes a simple PCR-only workflow (mPCR) to replace SB analysis, that incorporates novel procedural controls, treatment of the DNA in separate control and methylation-sensitive restriction endonuclease reactions, amplification with labeled primers, and two-color amplicon sizing by capillary electrophoresis. mPCR was evaluated in two independent laboratories with 76 residual clinical samples that represented typical and challenging fragile X alleles in both males and females. mPCR enabled superior size resolution and analytical sensitivity for size and methylation mosaicism compared to SB. Full mutation mosaicism was detected down to 1% in a background of 99% normal allele with 50- to 100-fold less DNA than required for SB. A low level of full mutation mosaicism in one sample was detected using mPCR but not observed using SB. Overall, the sensitivity for detection of full mutation alleles was 100% (95% CI: 89%-100%) with an accuracy of 99% (95% CI: 93%-100%). mPCR analysis of DNA from individuals with Klinefelter and Turner syndromes, and DNA from sperm and blood, were consistent with SB. As such, mPCR enables accurate, sensitive, and standardized methods of FMR1 analysis that can harmonize results across different laboratories.

  9. A simple explanation for a case of incompatibility with the reading frame theory in Duchenne muscular dystrophy: failure to detect an aberrant restriction fragment in Southern blot analysis.

    Science.gov (United States)

    Patria, S Y; Takeshima, Y; Suminaga, R; Nakamura, H; Iwasaki, R; Minagawa, T; Matsuo, M

    1999-09-01

    According to the translational reading frame theory, Duchenne muscular dystrophy (DMD) patients harbor out-of-frame deletion mutations in the dystrophin gene. We identified a Japanese DMD case who appeared to have an in-frame deletion of exons 46-54 that was disclosed by Southern blot analysis using a dystrophin cDNA as a probe. Analysis of dystrophin mRNA in skeletal muscle revealed the presence of an out-of-frame deletion of exons 46-53. In agreement with this result, the region encompassing exon 54 could be amplified from genomic DNA by polymerase chain reaction (PCR). Furthermore, re-analysis by Southern blot using an exon specific probe disclosed that a HindIII fragment containing exon 54 was present at aberrant size, leading to the incorrect conclusion that exon 54 had been deleted. Thus, this particular DMD case does not constitute an exception to the reading frame theory.

  10. Comparison of Southern blot analysis with isotopic and nonisotopic in situ hybridization for the detection of human papillomavirus sequences in invasive carcinoma of the uterine cervix.

    Science.gov (United States)

    D'Amato, L; Pilotti, S; Rotola, A; Di Luca, D; Cassai, E; Rilke, F

    1992-03-01

    To compare the efficiency of hybridization methods for the detection of HPV genome, 22 cases of invasive squamous cell carcinoma of the uterine cervix were analyzed by Southern blot analysis and in situ hybridization carried out with 35S- and biotin-labeled probes. These cases contained from less than one to as many as 50 copies per cell of HPV 16 and 18 types. To increase the sensitivity of biotinylated probes, a silver enhancement procedure of the peroxidase reaction product was applied. Results showed that in situ hybridization performed with isotopic probes is as sensitive as Southern blot analysis and is more sensitive than that performed with biotin-labeled probe. However, the application of the silver enhancement procedure increases the percentage of HPV-positive cases from 27 to 50%.

  11. Polymerase chain reaction and Southern blot-based analysis of the C9orf72 hexanucleotide repeat in different motor neuron diseases.

    Science.gov (United States)

    Hübers, Annemarie; Marroquin, Nicolai; Schmoll, Birgit; Vielhaber, Stefan; Just, Marlies; Mayer, Benjamin; Högel, Josef; Dorst, Johannes; Mertens, Thomas; Just, Walter; Aulitzky, Anna; Wais, Verena; Ludolph, Albert C; Kubisch, Christian; Weishaupt, Jochen H; Volk, Alexander E

    2014-05-01

    The GGGGCC-hexanucleotide repeat expansion in C9orf72 is the most common genetic cause of familial amyotrophic lateral sclerosis and frontotemporal dementia. This study determined the frequency of C9orf72 repeat expansions in different motor neuron diseases (amyotrophic lateral sclerosis (ALS), motor neuron diseases affecting primarily the first or the second motor neuron and hereditary spastic paraplegia). Whereas most studies on C9orf72 repeat expansions published so far rely on a polymerase chain reaction-based screening, we applied both polymerase chain reaction-based techniques and Southern blotting. Furthermore, we determined the sensitivity and specificity of Southern blotting of the C9orf72 hexanucleotide repeat in DNA derived from lymphoblastoid cell lines. C9orf72 repeat expansions were found in 27.1% out of 166 familial ALS patients, only once in 68 sporadic ALS patients, and not in 61 hereditary spastic paraplegia patients or 52 patients with motor neuron diseases affecting clinically primarily either the first or the second motor neuron. We found hints for a correlation between C9orf72 repeat length and the age of onset. Somatic instability of the C9orf72 repeat was observed in lymphoblastoid cell lines compared with DNA derived from whole blood from the same patient and therefore caution is warranted for repeat length determination in immortalized cell lines.

  12. 热带果树基因组DNA提取方法的改良及Southern blot分析%A Modified DNA Extraction Method for Tropical Fruit Trees and Southern Blot Analysis

    Institute of Scientific and Technical Information of China (English)

    彭军; 曾凡云; 龙海波; 黄俊生; 郭建荣

    2012-01-01

    Tissues of most tropical fruit trees contain polysaccharide and polyphenolics that restrict the extraction of high-quality genomie DNA for further molecular biology analysis. This experiment was conducted to develop a simple, universal and effective modified CTAB method for genomie DNA extraction from tissues of tropical fruit trees for southern blot analysis. This optimized procedure includes two steps. First, before the DNA was extrac- ted, the polysaccharide and polyphenolics were pre-removed through the STE lysis buffer by centrifugation. In addition, possible contamination by secondary metaboliates could be avoided by picking up the cotton-shaped ge- nomie DNA floccules resuspended in liquid at the DNA precipitation step with ethanol. With the modified CTAB method, high-quality DNAs were extracted from young leaves of the three representative tropical fruit trees, ba- nana (Musa spp. ) , papaya (Carica papaya L. ) and longan (Dimocarpus longan Lour. ). After digested with different restriction enzymes, fragments of banana ethylene receptor gene (GenBank accession No. AF113748), papaya phytoene desaturase gene (DQ779922) and longan flowering-related LEAFY homologous gene (DQ160214) were used as probes for further Southern blot analysis, respectively. The hybridization bands were clear with high background. The results indicate that the modified CTAB method, with pre-treated conjugation by DNA pick-up, is a simple and effective protocol for genomic DNA isolation from tropical fruit trees, which pro- duces high-quality genomic DNA available for further Southern blot analysis and other tests in molecular biology.%以香蕉、番木瓜、龙眼为代表材料,建立一套适合热带果树基因组DNA提取的方法——改良CTAB法。将提取各基因组DNA经限制性内切酶完全消化后,分别以香蕉内源乙烯受体基因(AF113748)、番木瓜八氢番茄红素脱氢酶基因PDS(DQ779922)、龙眼开花相关基因LEAFY(ADQ160214)为探针进行Southern

  13. Inverse PCR and Quantitative PCR as Alternative Methods to Southern Blotting Analysis to Assess Transgene Copy Number and Characterize the Integration Site in Transgenic Woody Plants.

    Science.gov (United States)

    Stefano, Biricolti; Patrizia, Bogani; Matteo, Cerboneschi; Massimo, Gori

    2016-06-01

    One of the major unanswered questions with respect to the commercial use of genetic transformation in woody plants is the stability of the transgene expression over several decades within the same individual. Gene expression is strongly affected by the copy number which has been integrated into the plant genome and by the local DNA features close to the integration sites. Because woody plants cannot be subjected to selfing or backcrossing to modify the transgenic allelic structure without affecting the valuable traits of the cultivar, molecular characterization of the transformation event is therefore crucial. After assessing the transgene copy number of a set of apple transgenic clones with Southern blotting, we describe two alternative methods: the first is based on inverse PCR (i-PCR) and the second on the quantitative PCR (q-PCR). The methods produced comparable results with the exception of the data regarding a high copy number clone, but while the q-PCR-based system is rapid and easily adaptable to high throughput systems, the i-PCR-based method can provide information regarding the transformation event and the characteristics of the sequences flanking the transgenic construct.

  14. Northern blotting analysis

    DEFF Research Database (Denmark)

    Josefsen, Knud; Nielsen, Henrik

    2011-01-01

    Northern blotting analysis is a classical method for analysis of the size and steady-state level of a specific RNA in a complex sample. In short, the RNA is size-fractionated by gel electrophoresis and transferred by blotting onto a membrane to which the RNA is covalently bound. Then, the membran...

  15. Northern blotting analysis

    DEFF Research Database (Denmark)

    Josefsen, Knud; Nielsen, Henrik

    2011-01-01

    is analysed by hybridization to one or more specific probes that are labelled for subsequent detection. Northern blotting is relatively simple to perform, inexpensive, and not plagued by artefacts. Recent developments of hybridization membranes and buffers have resulted in increased sensitivity closing...

  16. Uso da técnica de Southern Blot/Hibridização associada à reação em cadeia da polimerase para aumentar a sensibilidade no diagnóstico das infecções por hemoplasmas em gatos domésticos: Use of Southern Blot/Hybridization technique associated to polymerase chain reaction to improve the sensitivity in the diagnosis of hemoplasma infections in domestic cats

    Directory of Open Access Journals (Sweden)

    Daniel B. Macieira

    2009-12-01

    Full Text Available O objetivo deste trabalho foi verificar se a técnica de Southern Blot/Hibridização (SB em associação à reação de polimerização em cadeia (PCR aumenta a sensibilidade na detecção de DNA de hemoplasmas em gatos domésticos (Felis catus. O sangue total foi coletado em tubos contendo o anticoagulante ácido etilenodiamino tetra-acético, o DNA extraído a partir de 149 animais e a PCR realizada com o uso de sequências iniciadoras espécie-específicas, para amplificar subunidade 16S do RNA ribossomal de Mycoplasma haemofelis e 'Candidatus M. haemominutum' dessas amostras. Para a hibridização, foram utilizadas sondas específicas quimicamente marcadas, e os resultados visualizados por meio da adição de substrato quimiluminescente seguida de autoradiografia. Dezoito (12,1% das 149 amostras testadas apresentaram resultado PCR-positivo para o DNA de hemoplasmas. A técnica de SB mostrou que 24/149 (16,1% amostras apresentaram resultado positivo para hemoplasmas, confirmando os 18 resultados PCR-positivos, além de revelar seis outros adicionais (p The aim of this study was to determine whether Southern Blot/Hybridization (SB associated to Polymerase Chain Reaction (PCR improves the sensitivity in the detection of hemoplasma DNA in domestic cats (Felis catus. Whole blood was collected in tubes containing the anticoagulant ethylenediamine tetra-acetic acid and DNA extracted from 149 animals. PCR was performed using species specific primers to amplify the 16S ribosomal RNA subunit of Mycoplasma haemofelis and 'Candidatus M. haemominutum' from these samples. Hybridization was performed using a 16S rDNA probes chemically labeled and the results were visualized using a chemiluminescent substrate addition followed by autoradiography. Eighteen (12.1% of the 149 tested samples had a positive PCR result for hemoplasma species DNA. SB/hybridization technique showed that 24/149 (16.1% samples were positive for hemoplasmas, confirming the 18 PCR

  17. A fragile X male with a broad smear on southern blot analysis representing 100-500 CGG repeats and no methylation at the EagI site of the FMR-1 gene

    Energy Technology Data Exchange (ETDEWEB)

    Lachiewicz, A.M.; Spiridigliozzi, G.A.; McConkie-Rosell, A. [Duke Univ. Medical Center, Durham, NC (United States)] [and others

    1996-08-09

    Fragile X DNA studies were carried out on all obligate carriers of a large fragile X family with 10 mentally retarded individuals. One 64-year-old carrier man with an altered FMR-1 allele was not described as being mentally retarded or as having any limitations in function. He was married, raised 8 children, and worked as an auto mechanic. On examination, he had macrocephaly and mild macroorchidism but few of the other typical physical findings of males with fragile X syndrome. His Full Scale IQ is 73, and his Vineland Adaptive Behavior Composite is 73. On the Woodcock-Johnson Psycho-Educational Battery-Revised, he achieved standard scores of 64 in Reading, 55 in Math, and 83 in Knowledge. His DNA findings showed a broad smear on Southern blot analysis of 100-500 CGG repeats and no methylation at the EagI site upstream of the FMR-1 protein coding region. His FMR-1 protein production is 12% of normal. His daughters all have large premutations, with somatic instability in the size of the CGG repeat lengths. They all have evidence of academic underachievement and 2 have physical characteristics frequently described in individuals with fragile X. 21 refs., 3 figs.

  18. Genotypic analysis of cutaneous T-cell lymphoma: a comparative study of Southern blot analysis with polymerase chain reaction amplification of the T-cell receptor-gamma gene.

    Science.gov (United States)

    Curcó, N; Servitje, O; Llucià, M; Bertran, J; Limón, A; Carmona, M; Romagosa, V; Peyrí, J

    1997-11-01

    The diagnosis of early cutaneous T-cell lymphoma (CTCL) is a difficult point in dermatology. Recently, Southern blot analysis (SBA) and polymerase chain reaction (PCR) have been used to detect clonality in initial lesions in which clinical and histological findings are unspecific. Forty-one samples from 25 patients with CTCL were investigated for the presence of T-cell receptor-gamma gene rearrangement using a nested PCR technique and analysed by polyacrylamide gel electrophoresis (PAGE). Conventional SBA was also performed on 28 samples from 20 of these patients. In addition, 20 samples corresponding to patients with large plaque parapsoriasis (LPP), cutaneous B-cell lymphoma (CBCL) and eczema were analysed by PCR in the same way as were the CTCL specimens. Most of the CTCL specimens (81%) showed clonality on PCR analysis. Among patients with mycosis fungoides, 71% of initial patch lesions and 100% of plaques and tumours showed clonal disease. Clonality could be detected in three of four histologically negative post-treatment lesions. Clonal rearrangement was detected in one of three patients with LPP and in three of 10 patients with CBCL. None of the samples corresponding to patients with eczema showed positive results. SBA was significantly less sensitive than PCR in detecting clonality in CTCL patients (42% among early disease and 60% among advanced cases). The results indicate that this PCR/PAGE technique is a reliable and useful method for the detection of clonality in early skin lesions of CTCL patients and probably in the identification of silent extracutaneous involvement.

  19. Screening, Cloning and Southern Blotting Analysis of Specific Repetitive DNA Sequences of Psathyrostachys Huashanica%华山新麦草特异重复序列的筛选、克隆及Southern杂交分析

    Institute of Scientific and Technical Information of China (English)

    陈林刚; 武军; 赵继新; 刘淑会; 杨群慧; 杜万里; 庞玉辉; 陈新宏

    2010-01-01

    为了寻找华山新麦草特异重复序列,以华山新麦草、栽培一粒小麦、栽培二粒小麦、野生一粒小麦、野生二粒小麦、圆锥小麦、阿拉拉特小麦、茹科夫斯基小麦、斯卑尔脱小麦、普通小麦"中国春"(CS)为材料,利用200条RAPD引物筛选出11条华山新麦草特异条带(命名为RHS1~RHS11),并对这些条带进行回收、克隆、测序.将序列在NCBI数据库中进行比对,其中RHS1、RHS2、RHS3、RHS4、RHS6、RHS8、RHS9在NCBI数据库中未发现与其同源的序列.RHS5、RHS7、RHS10、 RHS11在NCBI数据库中有相似序列,其最高覆盖度和最高相似性分别为:83%和100%、99%和85%、49%和100%、19%和83%.通过Southern blotting进行序列特异性检测,RHS1、RHS2、RHS4、RHS6和RHS9等5个克隆在10种材料中都没有杂交信号;RHS7、RHS10和RHS11在华山新麦草及其他9种材料中都有弥散分布的杂交信号;RHS3、RHS5和RHS8只在华山新麦草中有杂交信号的序列.这些结果说明RHS3、RHS5、RHS8为华山新麦草基因组特异重复序列.

  20. 基于地高辛标记对小麦进行Southern杂交分析主要影响因素的优化和验证%Optimizing and Confirming of Digoxigenin Based Southern Blotting for Wheat Genome Analysis

    Institute of Scientific and Technical Information of China (English)

    刘禄; 牛焱焱; 雷昊; 林志珊; 赵翔宇; 叶兴国; 张宪省

    2012-01-01

    以转基因小麦和野生型小麦DNA为材料,对利用地高辛标记对小麦基因组DNA进行Southern杂交分析的影响因素进行了优化研究,包括探针制备与纯化、样品DNA量、酶切体系、真空转印条件、杂交条件、免疫检测方法等.结果表明,对随机引物标记的模板和标记后的探针进行纯化可明显提高探针的标记效率,10μg高质量的DNA样品在80μl的体系中,酶切8~12h可获得良好的效果;真空转膜时使用碱性液比中性液获得的转膜效果更干净;试剂纯度、杂交温度及杂交炉转速等均对杂交效果产生重要影响;配合改进的CSPD涂布方法,使用化学发光检测系统比单纯使用X光片显像更易操作,背景更干净;本研究所优化的地高辛标记的小麦Southern杂交分析显示出较高的灵敏度和信噪比,结果稳定,可克服同位素标记对实验条件、设备及实验人员身体状况等限制,在普通实验室推广应用.%The Southern blot analysis based on isotope labeling technique has some shortages such as facility limitation, environmental pollution, and poor stability. Therefore, establishing a safe, stable and efficient Southern hybridization protocol is very important to detect the integration of exogenous genes in transgenic wheat. In this study , by using the DNAs from wild-type wheat and transgenic wheat asmaterial, the DIG-labeled Southern blot analysis was improved through modifying several key steps, including probe preparation and purification, usage of DNA samples amount, digestion system, vacuum transfer conditions, stringent hybridization conditions, and immunoassay detect. The results showed that the purifications of DNA template and probe- labeled could significantly improve the efficiency of the probe labeling when random prime labeling method was used. Desirable digestion result could be a-chieved when 10|xg DNA samples with high quality was enzymed in 80|xl reaction system for 8 ~ 12

  1. Studying protein-protein interactions via blot overlay/far western blot.

    Science.gov (United States)

    Hall, Randy A

    2015-01-01

    Blot overlay is a useful method for studying protein-protein interactions. This technique involves fractionating proteins on SDS-PAGE, blotting to nitrocellulose or PVDF membrane, and then incubating with a probe of interest. The probe is typically a protein that is radiolabeled, biotinylated, or simply visualized with a specific antibody. When the probe is visualized via antibody detection, this technique is often referred to as "Far Western blot." Many different kinds of protein-protein interactions can be studied via blot overlay, and the method is applicable to screens for unknown protein-protein interactions as well as to the detailed characterization of known interactions.

  2. Single cell-resolution western blotting.

    Science.gov (United States)

    Kang, Chi-Chih; Yamauchi, Kevin A; Vlassakis, Julea; Sinkala, Elly; Duncombe, Todd A; Herr, Amy E

    2016-08-01

    This protocol describes how to perform western blotting on individual cells to measure cell-to-cell variation in protein expression levels and protein state. Like conventional western blotting, single-cell western blotting (scWB) is particularly useful for protein targets that lack selective antibodies (e.g., isoforms) and in cases in which background signal from intact cells is confounding. scWB is performed on a microdevice that comprises an array of microwells molded in a thin layer of a polyacrylamide gel (PAG). The gel layer functions as both a molecular sieving matrix during PAGE and a blotting scaffold during immunoprobing. scWB involves five main stages: (i) gravity settling of cells into microwells; (ii) chemical lysis of cells in each microwell; (iii) PAGE of each single-cell lysate; (iv) exposure of the gel to UV light to blot (immobilize) proteins to the gel matrix; and (v) in-gel immunoprobing of immobilized proteins. Multiplexing can be achieved by probing with antibody cocktails and using antibody stripping/reprobing techniques, enabling detection of 10+ proteins in each cell. We also describe microdevice fabrication for both uniform and pore-gradient microgels. To extend in-gel immunoprobing to gels of small pore size, we describe an optional gel de-cross-linking protocol for more effective introduction of antibodies into the gel layer. Once the microdevice has been fabricated, the assay can be completed in 4-6 h by microfluidic novices and it generates high-selectivity, multiplexed data from single cells. The technique is relevant when direct measurement of proteins in single cells is needed, with applications spanning the fundamental biosciences to applied biomedicine.

  3. TLC-Blot (Far-Eastern Blot) and Its Application to Functional Lipidomics.

    Science.gov (United States)

    Taki, Takao

    2015-01-01

    A simple method for transfer of lipids-including phospholipids, glycolipids, and neutral lipids-from a high performance thin layer chromatography (HPTLC) plate to a polyvinylidene difluoride (PVDF) membrane, TLC-Blot (Far-Eastern Blot), and its biochemical applications are presented. This chapter presents the conventional procedures for separating lipid from tissue samples, cultured cells, and serum and the subsequent development of TLC. Individual lipids separated on an HPTLC plate can be transferred to the PVDF membrane quantitatively and also isolated from the lipid-blotted membrane by a one-step purification procedure. Immunodetection with monoclonal antibodies and treatment with lipid-metabolizing enzymes on the lipid-blotted membrane are possible. The method for identification of individual lipids transferred on the PVDF membrane using matrix-assisted laser desorption/ionization quadrupole ion trap time-of-flight mass spectrometry (TLC-Blot/MALDI-TOF MS) is shown as a functional lipidomics application.

  4. Cloning, Southern blotting and RT-PCR analysis of a cDNA fragment encoding of 70 kDa heat shock cognate protein(Hsc70) from the oyster(Crassostrea ariakensis)%近江牡蛎Hsc70蛋白基因cDNA片段的克隆及Southern杂交和RT-PCR分析

    Institute of Scientific and Technical Information of China (English)

    张其中; 吴信忠; 高劲松; 潘金培

    2003-01-01

    In order to elucidate the molecular mechanisms of the oyster ( Crassostrea ariakensis) against adverse stimulating factors, we cloned and sequenced a partial cDNA encoding a 70 kDa heat shock cognate protein (Hsc70) from the oyster. The live oysters were obtained from Chengcun, Yangxi County, Guangdong Province, China. Various tissues, including mantle, gills, adductor muscle, heart and blood cells, were respectively collected from 5 untreated live oysters or treated ones at 36℃ for 1.5 hours, and immediately frozen in liquid nitrogen except for the blood cells which were suspended with Trizol Reagent after centrifugation (12 000 r/min for 30 s) and stored at - 20℃ . Total RNA was isolated using Trizol Reagent according to the manufacture's instructions. The first strand cDNA was synthesized using reverse transcriptase Superscript Ⅱ according to the manufacture's instructions. The primers were designed from a conserved region of C. gigas Hsc70 cDNA sequence (GeneBank accession No. AF144646). The polymerase chain reaction (PCR)was performed for 30 cycles with denaturation at 94℃ for 30 s, annealing at 49℃ for 40 s, and elongation at 72℃ for 30 s. The product was cloned to pGEM-T easy vector and sequenced. It is 509 base pairs (bp) and possesses 94 % identity with the cDNA encoding C. gigas Hsc70 using Blastn. This homology was strongly confirmed by amino acid sequence comparison using the Blastx (99 % ). The 509 bp fragment was labeled with α-32pdCTP and a random primer DNA labeling kit and employed as a probe to perform Southern blotting, the result demonstrated that the cDNA came from a partial mRNA transcript of C. ariakensis genomic DNA gene. The polymerase chain reaction (PCR) was carried out to investigate the expression of Hsc70, Using the cDNAs of several tissues, such as gills (heat shocked), mantle, adductor muscle (heat shocked), heart, blood cells (one sample with heat shock for 1.5 hours at 36℃ and another without any stimulus).The PCR

  5. Selected Reaction Monitoring (SRM) frente a Western Blot

    OpenAIRE

    Heredia Ponce, Zahira Maria; Urbano Gámez, Jose Alberto

    2016-01-01

    We propose the SRM technology as a complementary method to the Western Blot for the detection and quantification of proteins in a sample. The technique Western Blot has its own limitations: i) only a protein-of-choice is detected, ignoring any non-relevant proteins, ii) the sensitivity of the technique depends on the specificity of the antibody and iii) Western Blot is expensive and time-consuming. The advantages of SRM with respect Western Blot are remarkable: i) you can detect up to h...

  6. BLOTS AND ALL: A HISTORY OF THE RORSCHACH INK BLOT TEST IN BRITAIN.

    Science.gov (United States)

    Hubbard, Katherine; Hegarty, Peter

    2016-01-01

    Despite the easily recognizable nature of the Rorschach ink blot test very little is known about the history of the test in Britain. We attend to the oft-ignored history of the Rorschach test in Britain and compare it to its history in the US. Prior to the Second World War, Rorschach testing in Britain had attracted advocates and critiques. Afterward, the British Rorschach Forum, a network with a high proportion of women, developed around the Tavistock Institute in London and The Rorschach Newsletter. In 1968, the International Rorschach Congress was held in London but soon after the group became less exclusive, and fell into decline. A comparative account of the Rorschach in Britain demonstrates how different national institutions invested in the 'projective hypothesis' according to the influence of psychoanalysis, the adoption of a nationalized health system, and the social positioning of 'others' throughout the twentieth century. In comparing and contrasting the history of the Rorschach in Britain and the US, we decentralize and particularize the history of North American Psychology.

  7. Fast and Simple micro-RNA Northern Blots

    Directory of Open Access Journals (Sweden)

    Nham Tran

    2009-01-01

    Full Text Available RNA northern blots provide robust measurements of gene expression. The simple northern blot technique described in this report has been optimised to provide rapid, reproducible detection and analysis of mature and precursor forms of microRNAs. This protocol economises on the use of commercially available components and secondly reduces the hybridisation step to 2 hours.

  8. The Design of a Quantitative Western Blot Experiment

    Directory of Open Access Journals (Sweden)

    Sean C. Taylor

    2014-01-01

    Full Text Available Western blotting is a technique that has been in practice for more than three decades that began as a means of detecting a protein target in a complex sample. Although there have been significant advances in both the imaging and reagent technologies to improve sensitivity, dynamic range of detection, and the applicability of multiplexed target detection, the basic technique has remained essentially unchanged. In the past, western blotting was used simply to detect a specific target protein in a complex mixture, but now journal editors and reviewers are requesting the quantitative interpretation of western blot data in terms of fold changes in protein expression between samples. The calculations are based on the differential densitometry of the associated chemiluminescent and/or fluorescent signals from the blots and this now requires a fundamental shift in the experimental methodology, acquisition, and interpretation of the data. We have recently published an updated approach to produce quantitative densitometric data from western blots (Taylor et al., 2013 and here we summarize the complete western blot workflow with a focus on sample preparation and data analysis for quantitative western blotting.

  9. Mixed lubrication after rewetting of blotted pleural mesothelium.

    Science.gov (United States)

    Bodega, Francesca; Sironi, Chiara; Porta, Cristina; Pecchiari, Matteo; Zocchi, Luciano; Agostoni, Emilio

    2013-01-15

    Coefficient of kinetic friction (μ) of pleural mesothelium blotted with filter paper, and rewetted with Ringer solution markedly increases; this increase is removed if a sufficient amount of sialomucin or hyaluronan is added to Ringer (Bodega et al., 2012. Respiratory Physiology and Neurobiology 180, 34-39). In this research we found that μ of pleural mesothelium blotted, rewetted, and sliding at physiological velocities and loads, decreased with increase of velocity, mainly at low velocities. Despite this decrease, μ at highest velocity was still double that before blotting. With small concentration of sialomucin or hyaluronan μ was markedly smaller at each velocity, decreased less with increase of velocity, and at highest velocity approached preblotting value. These findings indicate a regime of mixed lubrication in post-blotting Ringer, at variance with boundary lubrication occurring before blotting or postblotting with sufficient macromolecule addition. Greater roughness of mesothelial surface, caused by blotting, likely induces zones of elastohydrodynamic lubrication, which increase with velocity, while contact area decreases.

  10. The line blot assay: problems with titrating first and second antibodies for Western blot and immunohistochemistry assays?

    Science.gov (United States)

    Rojas-Espinosa, O; Silva-Miranda, M; Wek-Rodriguez, K; Arce-Paredes, P

    2006-01-01

    We describe a technique designed to assess the optimal dilution of primary and secondary antibodies, to be used in Western blot, dot blot, the multi-antigen print immunoassay (MAPIA) and immunohistochemistry assays. The method that we call "line blot" is not an alternative but a practical, complementary tool for the above techniques that assures definitive results are obtained from single assays, so there is no need to repeat the assay. As with most immunoenzymatic assays, the line blot assay is very sensitive, allowing the detection of absolute amounts of antigen as low as 2.5 ng in the 0.5 cm-long segment line (see Results), depending on the strength of the secondary, enzyme-labelled antibody.

  11. Routine Western blot to check autophagic flux : Cautions and recommendations

    NARCIS (Netherlands)

    Gomez-Sanchez, Ruben; Pizarro-Estrella, Elisa; Yakhine-Diop, Sokhna M. S.; Rodriguez-Arribas, Mario; Bravo-San Pedro, Jose M.; Fuentes, Jose M.; Gonzalez-Polo, Rosa A.

    2015-01-01

    At present, the analysis of autophagic flux by Western blotting (WB), which measures two of the most important markers of autophagy, i.e., microtubule-associated protein 1 light chain 3 (LC3) and p62, is widely accepted in the scientific community. In this study, we addressed the possible disadvanta

  12. Determination of Diagnostic Antigens in Cattle Amphistomiasis Using Western Blotting

    Directory of Open Access Journals (Sweden)

    A Halajian

    2009-05-01

    Full Text Available "nBackground: Mixed infection with amphistomes seems common in native cattle of Iran. The aim of this study was to determine diagnostic antigens in cattle mixed amphistomiasis."nMethods: Specific antigens of Cotylophoron cotylophorum, Gastrothylax crumenifer and Paramphisto­mum cervi (mixed infection, the most common species, were collected from cattle was deter­mined. Adult trematodes were collected from the rumen of naturally infected cattle at meat inspec­tion. After their homogenization and centrifugation, somatic antigens were prepared and ana­lyzed by SDS-PAGE. Specific antigens were determinated by western blot with homologous and heterolo­gous sera. SDS-PAGE of whole worms extract was performed at different concentrations and subse­quent gels staining. Immunoblotting analysis using sera from cattle naturally infected with am­phistomes, Dicrocoelium dendriticum, Fasciola spp. and hydatid cyst was performed."nResults: Electrophorese analysis of somatic antigens revealed the presence of 10 and 21 protein bands at 4 µgr/ml and 8 µgr/ml with molecular weights ranging from 25-120 and 25-150 kDa, respectively. The best result was taken at 8 mg/ml concentration. Although western blot of these proteins demon­strate 5 major antigenic polypeptides ranging from 50 to 100 kDa which were recognized by serum of cat­tle naturally infected with mixed amphistomes.

  13. Spectrophotometric quantitation of DNA on blots after ethanol-solubilization of the MTT-formazan from anti-digoxigenin-based detection of nucleic acids.

    Science.gov (United States)

    Colgan, D J

    1993-01-01

    The tetrazolium salt, 3-(4,5-dimethyl-thiazolyl-2)-2,5-diphenyl tetrazolium bromide (MTT) has recently been established as a substitute for Nitro-blue tetrazolium (NBT) in stain mixtures using antibody-conjugated alkaline phosphatase for the location of proteins on Western blots (Heegaard, 1990). Experiments reported here show that MTT is as sensitive as NBT in digoxigenin-labeled probe localization (on nucleic acid blots) utilizing alkaline-phosphatase-labelled, anti-digoxigenin antibodies. Moreover, as the formazan from MTT is soluble in ethanol, it is shown that spectrophotometric quantitation can be used to estimate the amount of target DNA on dot and Southern blots. For dot blotting, pBR328 was used as the probe and pBR322 as target. For Southern blots, human rDNA was used as the probe and total genomic calf DNA as the target. Staining response was linear over at least six twofold DNA dilutions in both types of blot.

  14. Antibody performance in western blot applications is context-dependent.

    Science.gov (United States)

    Algenäs, Cajsa; Agaton, Charlotta; Fagerberg, Linn; Asplund, Anna; Björling, Lisa; Björling, Erik; Kampf, Caroline; Lundberg, Emma; Nilsson, Peter; Persson, Anja; Wester, Kenneth; Pontén, Fredrik; Wernérus, Henrik; Uhlén, Mathias; Ottosson Takanen, Jenny; Hober, Sophia

    2014-03-01

    An important concern for the use of antibodies in various applications, such as western blot (WB) or immunohistochemistry (IHC), is specificity. This calls for systematic validations using well-designed conditions. Here, we have analyzed 13 000 antibodies using western blot with lysates from human cell lines, tissues, and plasma. Standardized stratification showed that 45% of the antibodies yielded supportive staining, and the rest either no staining (12%) or protein bands of wrong size (43%). A comparative study of WB and IHC showed that the performance of antibodies is application-specific, although a correlation between no WB staining and weak IHC staining could be seen. To investigate the influence of protein abundance on the apparent specificity of the antibody, new WB analyses were performed for 1369 genes that gave unsupportive WBs in the initial screening using cell lysates with overexpressed full-length proteins. Then, more than 82% of the antibodies yielded a specific band corresponding to the full-length protein. Hence, the vast majority of the antibodies (90%) used in this study specifically recognize the target protein when present at sufficiently high levels. This demonstrates the context- and application-dependence of antibody validation and emphasizes that caution is needed when annotating binding reagents as specific or cross-reactive. WB is one of the most commonly used methods for validation of antibodies. Our data implicate that solely using one platform for antibody validation might give misleading information and therefore at least one additional method should be used to verify the achieved data.

  15. Cy5 total protein normalization in Western blot analysis.

    Science.gov (United States)

    Hagner-McWhirter, Åsa; Laurin, Ylva; Larsson, Anita; Bjerneld, Erik J; Rönn, Ola

    2015-10-01

    Western blotting is a widely used method for analyzing specific target proteins in complex protein samples. Housekeeping proteins are often used for normalization to correct for uneven sample loads, but these require careful validation since expression levels may vary with cell type and treatment. We present a new, more reliable method for normalization using Cy5-prelabeled total protein as a loading control. We used a prelabeling protocol based on Cy5 N-hydroxysuccinimide ester labeling that produces a linear signal response. We obtained a low coefficient of variation (CV) of 7% between the ratio of extracellular signal-regulated kinase (ERK1/2) target to Cy5 total protein control signals over the whole loading range from 2.5 to 20.0μg of Chinese hamster ovary cell lysate protein. Corresponding experiments using actin or tubulin as controls for normalization resulted in CVs of 13 and 18%, respectively. Glyceraldehyde-3-phosphate dehydrogenase did not produce a proportional signal and was not suitable for normalization in these cells. A comparison of ERK1/2 signals from labeled and unlabeled samples showed that Cy5 prelabeling did not affect antibody binding. By using total protein normalization we analyzed PP2A and Smad2/3 levels with high confidence.

  16. Characterization of Nora Virus Structural Proteins via Western Blot Analysis

    Directory of Open Access Journals (Sweden)

    Brad L. Ericson

    2016-01-01

    Full Text Available Nora virus is a single stranded RNA picorna-like virus with four open reading frames (ORFs. The coding potentials of the ORFs are not fully characterized, but ORF3 and ORF4 are believed to encode the capsid proteins (VP3, VP4a, VP4b, and VP4c comprising the virion. To determine the polypeptide composition of Nora virus virions, polypeptides from purified virus were compared to polypeptides detected in Nora virus infected Drosophila melanogaster. Nora virus was purified from infected flies and used to challenge mice for the production of antisera. ORF3, ORF4a, ORF4b, and ORF4c were individually cloned and expressed in E. coli; resultant recombinant proteins purified and were used to make monospecific antisera. Antisera were evaluated via Western blot against whole virus particles and Nora virus infected fly lysates. Viral purification yielded two particle types with densities of ~1.31 g/mL (empty particles and ~1.33 g/mL (complete virions. Comparison of purified virus polypeptide composition to Nora virus infected D. melanogaster lysate showed the number of proteins in infected cell lysates is less than purified virus. Our results suggest the virion is composed of 6 polypeptides, VP3, VP4a, two forms of VP4b, and two forms of VP4c. This polypeptide composition is similar to other small RNA insect viruses.

  17. RNase protection assays and RNA gel blots: a direct comparison of sensitivity.

    Science.gov (United States)

    Higgs, D C; Colbert, J T

    1992-01-01

    RNase protection assays are commonly thought to be a more sensitive means of detecting and quantitating specific mRNAs than are RNA gel blots (Northern blots). We have directly compared the sensitivity of these two approaches by assaying for known amounts of in vitro synthesized beta-glucuronidase mRNA. With the probes and protocols employed here, the ability to detect a specific mRNA was similar whether RNase protection or RNA gel blot analyses were performed.

  18. RT-PCR and Northern blot analysis in search for a putative Paramecium beta-adrenergic receptor.

    Science.gov (United States)

    Płatek, A; Wiejak, J; Wyroba, E

    1999-01-01

    RT-PCR and Northern blot analysis were performed in order to search for a putative beta-adrenergic receptor (beta-AR) in Paramecium using several beta2-adrenergic-specific molecular probes. Under strictly defined RT-PCR conditions DNA species of expected molecular size about 360 bp were generated with the primers corresponding to the universal mammalian beta2-AR sequence tagged sites (located within the 4th and the 6th transmembrane regions of the receptor). This RT-PCR product hybridized in Southern blot analysis with the oligonucleotide probe designed to the highly conservative beta2-AR region involved in G-proteins interaction and located within the amplified region. Northern hybridization was performed on Paramecium total RNA and mRNA with human beta2-AR cDNA and two oligonucleotide probes: the first included Phe 290 involved in agonist binding (Strader et al., 1995) and the second was the backward RT-PCR primer. All these probes revealed the presence of about 2 kb mRNA which is consistent with the size of beta2-AR transcripts found in higher eukaryotes.

  19. Evaluation of an indigenous western blot kit for human immunodeficiency virus

    Directory of Open Access Journals (Sweden)

    Lakshmi V

    2002-01-01

    Full Text Available PURPOSE: The Western Blot test is considered a gold standard test for the confirmation of an ELISA and/or rapid assay screened reactive sample in the diagnosis of HIV infection, especially in the low risk population. In this study, an indigenously developed HIV W. Blot kit (J.Mitra & Co., New Delhi, India was compared for its performance characteristics with a widely used Western Blot kit, HIV Blot 2.2 (Genelabs, Singapore. Antigens of both HIV-1 and the indicator antigen gp36 of HIV-2 are included in the strips. METHODS: A panel of 150 clinical serum samples was used in the evaluation. All the sera were tested simultaneously by both the kits. RESULTS: The HIV W. Blot kit had high performance characteristics (100% sensitivity and 100% specificity, like the HIV Blot 2.2. The test procedure was easy to perform. There was clear delineation of the bands. CONCLUSIONS: The interpretation of the results on the HIV W. Blot was less prone to subjective errors. The test gave positive bands at even very high serum dilutions in the test kit. This fact indicates that HIV W. Blot probably has a potential application in early phases of infection, when the antibody concentrations are still very low.

  20. Immunochemische detectiemethoden na western blotting van cytochroom P-450 iso-enzymen

    NARCIS (Netherlands)

    Laan CA; Jansen EHJM

    1992-01-01

    In this report a number of staining techniques on Western blots have been compared with respect to sensitivity, background staining, practical applicability and cost aspects. After electrophoresis of a rat microsomal liver sample followed by blotting, an incubation was performed of a primary antibo

  1. Zinc Blotting Assay for Detection of Zinc-Binding Prolamin in Barley (Hordeum vulgare) Grain

    DEFF Research Database (Denmark)

    Uddin, Mohammad Nasir; Langkilde, Ane; Vincze, Éva

    2014-01-01

    In plants, zinc is commonly found bound to proteins. In barley (Hordeum vulgare), major storage proteins are alcohol-soluble prolamins known as hordeins, and some of them have the potential to bind or store zinc. 65Zn overlay and blotting techniques have been widely used for detecting zinc......-binding protein. However, to our knowledge so far this zinc blotting assay has never been applied to detect a prolamin fraction in barley grains. A radioactive zinc (65ZnCl2) blotting technique was optimized to detect zinc-binding prolamins, followed by development of an easy-to-follow nonradioactive colorimetric...... zinc blotting method with a zinc-sensing dye, dithizone. Hordeins were extracted from mature barley grain, separated by SDS-PAGE, blotted on a membrane, renatured, overlaid, and probed with zinc; subsequently, zinc-binding specificity of certain proteins was detected either by autoradiography or color...

  2. GTP-blot analysis of small GTP-binding proteins. The C-terminus is involved in renaturation of blotted proteins.

    Science.gov (United States)

    Klinz, F J

    1994-10-01

    Recombinant c-Ha-ras, ralA and rap2, but not rap1A or rap1B proteins retained their ability to bind [alpha-32P]GTP after SDS/PAGE and transfer to nitrocellulose. Recombinant c-Has-ras missing the C-terminal 23 amino acid residues failed to bind [alpha-32P]GTP after the blot, and the ability of recombinant ralA missing the C-terminal 28 amino acid residues to bind [alpha-32P]GTP was decreased many-fold. The presence of nonionic detergents of the polyoxyethylene type such as Tween 20, Triton X-100, Nonidet P40 or Lubrol PX in the incubation buffer was necessary to induce renaturation of blotted recombinant c-Ha-ras protein, whereas other types of detergents were ineffective. We propose that detergents of the polyoxyethylene type induce the refolding of some types of blotted small GTP-binding proteins and that the C-terminus is involved in the refolding process. Membranes from NIH3T3 fibroblasts overexpressing c-Ha-ras protein showed much weaker binding of [alpha-32P]GTP as expected from the level of ras immunoreactivity. Treatment of fibroblasts with lovastatin, an inhibitor of hydroxymethylglutaryl-coenzyme A reductase, caused the accumulation of the unfarnesylated form of c-Ha-ras in the cytosol. Examination of [alpha-32P]GTP-binding and immunoreactivity for cytosolic and membrane-bound c-Ha-ras revealed that binding of [alpha-32P]GTP to unprocessed c-Ha-ras was increased about threefold compared to the same amount of processed c-Ha-ras. Our results demonstrate that detection and quantification of small GTP-binding proteins in eukaryotic cells by GTP-blot analysis is hampered by the fact that these proteins differ strongly in their ability to renature after blotting to nitrocellulose.

  3. Accuracy of Reverse Dot-Blot PCR in Detection of Different β-Globin Gene Mutations.

    Science.gov (United States)

    El-Fadaly, N; Abd-Elhameed, A; Abd-Elbar, E; El-Shanshory, M

    2016-06-01

    Prevention programs for β-thalassemia based on molecular diagnosis of heterozygous carriers and/or patients require the use of reliable mutation screening methods. The aim of this study was to compare between direct DNA sequencing, and reverse dot-blot PCR in detection of different β-globin gene mutations in Egyptian children with β-thalassemia. Forty children with β-thalassemia were subjected to mutation analysis, performed by both direct DNA sequencing and β-globin Strip Assay MED™ (based on reverse dot-blot PCR). The most frequent mutant alleles detected by reverse dot-blot PCR were; IVSI-110 G>A (31.25 %), IVS I-6 T > C (21.25 %), and IVS I-1 G>A (20 %). Relatively less frequent mutant alleles detected by reverse dot-blot PCR were "IVSII-1 G>A (5 %), IVSII-745 C>G (5 %), IVSII-848 C>A (2.5 %), IVSI-5 G>C (2.5 %), -87 C>G(2.5 %), and cd39 C>T (2.5 %)", While the genotypes of three patients (6 alleles 7.5 %) were not detected by reverse dot-blot PCR. Mutant alleles detected by direct DNA sequencing were the same as reverse dot-blot PCR method except it revealed the genotypes of 3 undetected patients (one patient was homozygous IVSI-110 G>A, and two patients were homozygous IVS I-1 G>A. Sensitivity of the reverse dot-blot PCR was 92.5 % when compared to direct DNA sequencing for detecting β-thalassemia mutations. Our results therefore suggest that, direct DNA sequencing may be preferred over reverse dot-blot PCR in critical diagnostic situations like genetic counseling for prenatal diagnosis.

  4. CRITERIA OF POSITIVITY FOR Ig ANTIBODIES IN THE METHOD OF IMMUNE BLOTTING OF LYME DISEASE

    Directory of Open Access Journals (Sweden)

    V G Barskova

    2001-01-01

    Full Text Available There are currently no accepted criteria for positive Western blots in Russian patients with Lyme borreliosis. The purpose of the current study was to develop criteria for a positive IgG westem-blot to aid particularly in the diagnosis of patients with joint manifestation of the disorder. Patients: 97 with Lyme disease, 145 - control subjects. IgG antibody responses were determined to 3 species ofB.burgdorferi sensu lato by Western blotting, using blots prepared by manufacturer. The best discriminatory ability of test criteria was chained by requiring any 3 of 11 IgG bands, a definition that could be used with B. burgdorferi sensu stricto, B.garinii and B.afzelii strains. With these 3 antigen preparation, positive IgG blots were found in 0 to 18% of patients with localized erythema migrans of < 4 weeks duration, 23 to 39% of those with disseminated infection < 20 weeks duration, and in 39 to 46% of those with late arthritis/arthralgia of >6 months duration the specificity was 93 to 99%. Thus, IgG Western blotting may bring greater specificity to serologic testing in Lyme borreliosis, but the sensitivity is limited.

  5. Detection of Babesia and Theileria species infection in cattle from Portugal using a reverse line blotting method.

    Science.gov (United States)

    Silva, M G; Marques, P X; Oliva, A

    2010-12-15

    Babesiosis and Theileriosis are tick-borne diseases widespread in tropical and sub-tropical regions with high economic impact worldwide. In Portugal there are at least 4 tick vectors known to be competent for the transmission of Babesia and Theileria sp. identified: Rhipicephalus bursa, Rhipicephalus (Boophilus) annulatus, Ixodes ricinus and Haemaphysalis punctata. All these potential Babesia and Theileria tick vectors are widely distributed in Portugal, although they are predominant in the Southern region. In this study, 1104 cattle blood samples were randomly collected from Central and Southern regions of Portugal and analyzed by PCR-reverse line blotting (RLB) for the detection of Babesia and Theileria sp. Testing indicated that 74.7% of the bovines tested were positive for either Babesia and/or Theileria sp. In addition, five different apicomplexan species, namely, Theileria buffeli, Theileria annulata, Babesia divergens, Babesia bovis, and Babesia bigemina were detected by RLB among the bovines tested. T. buffeli was the most frequently found species, being present in 69.9% of the positive samples either as single infections (52.4%), or as mixed infections (17.5%). The Babesia specie most frequently found was B. divergens, detected in 4.2% of the infected bovines. Overall, infected bovines were found in all regions tested; however the highest number of infected bovines was observed in Évora district (96.2%) and in cattle from Limousin breeds (81.7%). The results indicate widespread Babesia and Theileria infections in Portuguese bovines, suggesting the need for improved control of ticks and tick-borne diseases.

  6. HOUSE DUST MITE ALLERGEN (Derp1 AND Blot5) LEVELS IN ASTHMATICS' HOME IN HONGKONG

    Institute of Scientific and Technical Information of China (English)

    Bao-qing Sun; Adrian Wu; Albert Chan; Stanley Chik; Dorothy Wong; Nan-shan Zhong

    2004-01-01

    Objective To measure Derpl and Blot5 allergen levels in asthmatics' homes in Hongkong.Methods Seventy houses were enrolled for a mite indoor environment study. Dust samples were obtained from two sites of each patients' house: bed and floor. Derpl and Blot5 levels were quantified by a two-site monoclonal antibody-based ELISA technique.Results The levels of Derpl allergens found in bed (geometric mean (GM) 3.43 μg/g of dust; 95%CI, 1.89-4.96 μg/g)and on the floor (GM 1.12 μg/g of dust; 95%CI, 0.71-1.53 μg/g) indicated significant differences (P=0.005). However, the levels of Blot5 allergens found in bed (GM 19.00 μg/g of dust; 95%CI, 0.89-38.90 μg/g) and on the floor (GM 6.14 μg/g of dust; 95%CI, 0.40-11.90 μg/g) showed no statistically significant difference. In addition, in regards to the exposure index for Derpl and Blot5 allergens found in bed and on the floor, 17.6% in bed and 8.6% on the floor had levels of Blot5 ≥ 10 μg/g of dust, higher than those obtained for Derp1 (7.2% and 0% in bed and on the floor respectively, P< 0.05); higher percentages in bed and on the floor (25.0% and 35.7%) were observed for levels of Blot5 =0 μg/g of dust as compared with Derpl in bed and on the floor (4.3% and 14.5% respectively, P< 0.05).Conclusions Derpl and Blot5 are the major allergens found in this regional study, Blot5 is a more potent allergen in Hongkong, probably reflecting the high level of exposure to Blomia tropicalis (Bt). Bt and Dermatophagoides pteronyssinus (Dp) allergens should be included for precise diagnosis and effective immuno-therapeutic treatment of mite allergy in Hongkong.

  7. Total protein or high-abundance protein: Which offers the best loading control for Western blotting?

    Science.gov (United States)

    Thacker, Jonathan S; Yeung, Derrick H; Staines, W Richard; Mielke, John G

    2016-03-01

    Western blotting routinely involves a control for variability in the amount of protein across immunoblot lanes. Normalizing a target signal to one found for an abundantly expressed protein is widely regarded as a reliable loading control; however, this approach is being increasingly questioned. As a result, we compared blotting for two high-abundance proteins (actin and glyceraldehyde 3-phosphate dehydrogenase [GAPDH]) and two total protein membrane staining methods (Ponceau and Coomassie Brilliant Blue) to determine the best control for loading variability. We found that Ponceau staining optimally balanced accuracy and precision, and we suggest that this approach be considered as an alternative to normalizing with a high-abundance protein.

  8. Indeterminate HIV-1 Western Blots: Etiology, Natural History, and Psychological Reactions

    Science.gov (United States)

    1992-09-16

    systemic lupus erythematosus, rheumatoid arthritis, juvenile onset diabetes mellitus , Sjogren’s disease, thyroiditis, and Crohn’s disease 4 TABLE 8 45...Cat wound ply 0-no; 1-scratched only; 2-bitten only; 3-both BE.2Bb Owned other pets 0-no;l- dog ; 2-rodent;3-other A/< . 2 # BE.3 SMOKING HISTORY 53 BE...blot. Sera from 26 cases with a pet cat were tested for reactivity on Western blots for feline leukemia virus (FeLV) and for feline immunodeficiency

  9. A Streamlined Western Blot Exercise: An Efficient and Greener Approach in the Laboratory Classroom

    Science.gov (United States)

    Ness, Traci L.; Robinson, Rebekah L.; Mojadedi, Wais; Peavy, Lydia; Weiland, Mitch H.

    2015-01-01

    SDS-PAGE and western blotting are two commonly taught protein detection techniques in biochemistry and molecular biology laboratory classrooms. A pitfall associated with incorporating these techniques into the laboratory is the significant wait times that do not allow students to obtain timely results. The waiting associated with SDS-PAGE comes…

  10. A Study of Rubisco through Western Blotting and Tissue Printing Techniques

    Science.gov (United States)

    Ma, Zhong; Cooper, Cynthia; Kim, Hyun-Joo; Janick-Buckner, Diane

    2009-01-01

    We describe a laboratory exercise developed for a cell biology course for second-year undergraduate biology majors. It was designed to introduce undergraduates to the basic molecular biology techniques of Western blotting and immunodetection coupled with the technique of tissue printing in detecting the presence, relative abundance, and…

  11. Ferulic acid enhances IgE binding to peanut allergens in western blots.

    Science.gov (United States)

    Phenolic compounds at high concentrations are known to form insoluble complexes with proteins. We hypothesized that this complex formation could interfere with Western blot and ELISA assays for peanut allergens. To verify this, three simple phenolic compounds (ferulic, caffeic, and chlorogenic acids...

  12. PET-blot analysis contributes to BSE strain recognition in C57Bl/6 mice.

    Science.gov (United States)

    Lezmi, Stéphane; Bencsik, Anna; Baron, Thierry

    2006-10-01

    Identification of the strain of agent responsible for bovine spongiform encephalopathy (BSE) can be made histologically through the analysis of both distribution and intensity of brain vacuolar lesions after BSE transmission to mouse. Another useful way to distinguish the BSE agent from other prion strains is the study of the distribution of the abnormal prion protein (PrP(res)). For that purpose, paraffin-embedded tissue blot (PET-blot) method was applied on brains from C57Bl/6 mice infected with cattle BSE, experimental sheep BSE, or feline spongiform encephalopathy (FSE) from a cheetah. PrP(res) distribution was comparable, whichever of the three BSE agent sources was considered and was distinct from the PrP(res) distribution in C57Bl/6 mice inoculated with a French scrapie isolate or with a mouse-adapted scrapie strain (C506M3). These data confirm a common origin of infectious agent responsible for the British and French cattle BSE. They also indicate that PET-blot method appears as a precise complementary tool in prion strain studies because it offers easy and quick assessment of the PrP(res) mapping. Advantages and limits of the PET-blot method are discussed and compared with other established and validated methods of strain typing.

  13. Combined use of Western blot/ELISA to improve the serological diagnosis of human tuberculosis

    Directory of Open Access Journals (Sweden)

    S. T. Beck

    2005-02-01

    Full Text Available Two recombinant antigens and a crude bacterial antigen of a wild M. tuberculosis strain were used to detect specific IgG antibodies in sera from 52 patients with pulmonary tuberculosis, confirmed by an acid-fast smear and serum culture of these patients and that of 25 contacts. The patients were not infected with HIV. We evaluated the sensitivity and specificity of ELISA, based on the recombinant TbF6® and TbF6/DPEP antigen and a search for reactivity patterns in the Western blot technique, using whole mycobacterium antigen. Serum samples from 22 healthy individuals and from 30 patients with lung diseases other than tuberculosis were used as controls. The best ELISA results were obtained with the TbF6/DPEP antigen combination, which gave 85% sensitivity and 91% specificity. ELISA sensitivity improved from 85% to 92% when the Western blot results were used. Western blot specificity was 100% when antibody reactivity with different antigenic bands was analyzed and associated. The association of TbF6/DPEP antigens used in ELISA with specific patterns of reactivity determined by Western blot can help make an identification when classic methods for the diagnosis of pulmonary tuberculosis are not sufficient.

  14. Effects of Reusing Gel Electrophoresis and Electrotransfer Buffers on Western Blotting

    Science.gov (United States)

    Omotola, Oluwabukola B.; Heda, Rajiv P.; Avery, Jamie

    2016-01-01

    SDS-PAGE and Western blotting are 2 of the most commonly used biochemical methods for protein analysis. Proteins are electrophoretically separated based on their MWs by SDS-PAGE and then electrotransferred to a solid membrane surface for subsequent protein-specific analysis by immunoblotting, a procedure commonly known as Western blotting. Both of these procedures use a salt-based buffer, with the latter procedure consisting of methanol as an additive known for its toxicity. Previous reports present a contradictory view in favor or against reusing electrotransfer buffer, also known as Towbin’s transfer buffer (TTB), with an aim to reduce the toxic waste. In this report, we present a detailed analysis of not only reusing TTB but also gel electrophoresis buffer (EB) on proteins of low to high MW range. Our results suggest that EB can be reused for at least 5 times without compromising the electrophoretic separation of mixture of proteins in an MW standard, BSA, and crude cell lysates. Additionally, reuse of EB did not affect the quality of subsequent Western blots. Successive reuse of TTB, on the other hand, diminished the signal of proteins of different MWs in a protein standard and a high MW membrane protein cystic fibrosis transmembrane-conductance regulator (CFTR) in Western blotting. PMID:27582639

  15. Miniaturized fluorescent RNA dot blot method for rapid quantitation of gene expression

    Directory of Open Access Journals (Sweden)

    Yadetie Fekadu

    2004-06-01

    Full Text Available Abstract Background RNA dot blot hybridization is a commonly used technique for gene expression assays. However, membrane based RNA dot/slot blot hybridization is time consuming, requires large amounts of RNA, and is less suited for parallel assays of more than one gene at a time. Here, we describe a glass-slide based miniaturized RNA dot blot (RNA array procedure for rapid and parallel gene expression analysis using fluorescently labeled probes. Results RNA arrays were prepared by simple manual spotting of RNA onto amino-silane coated microarray glass slides, and used for two-color fluorescent hybridization with specific probes labeled with Cy3 and 18S ribosomal RNA house-keeping gene probe labeled with Cy5 fluorescent dyes. After hybridization, arrays were scanned on a fluorescent microarray scanner and images analyzed using microarray image analysis software. We demonstrate that this method gives comparable results to Northern blot analysis, and enables high throughput quantification of transcripts from nanogram quantities of total RNA in hundreds of samples. Conclusion RNA array on glass slide and detection by fluorescently labeled probes can be used for rapid and parallel gene expression analysis. The method is particularly well suited for gene expression assays that involve quantitation of many transcripts in large numbers of samples.

  16. Western blotting as a method for studying cell-biomaterial interactions : The role of protein collection

    NARCIS (Netherlands)

    van Kooten, TG; Klein, CL; Kirkpatrick, CJ

    2001-01-01

    Research of cell-biomaterial interactions is building on knowledge and methods available in cell and molecular biology. Western blotting is one of the options to characterize protein expression in cell populations. Method transfer to biomaterial model systems is not trivial because of the structure

  17. Biofilm detection by wound blotting can predict slough development in pressure ulcers: A prospective observational study.

    Science.gov (United States)

    Nakagami, Gojiro; Schultz, Gregory; Gibson, Daniel J; Phillips, Priscilla; Kitamura, Aya; Minematsu, Takeo; Miyagaki, Tomomitsu; Hayashi, Akitatsu; Sasaki, Sanae; Sugama, Junko; Sanada, Hiromi

    2016-12-26

    Bacteria have been found to form multicellular aggregates which have collectively been termed "biofilms." It is hypothesized that biofilm formation is a means to protect bacterial cells including protection form the immune response of humans. This protective mechanism is believed to explain persistent chronic wound infections. At times, the biofilms are abundant enough to see, and remove by simple wiping. However, recent evidence has shown that the removal of these visible portions are not sufficient, and that biofilms can continue to form even with daily wiping. In this work, we tested an approach to detect the biofilms which are present after clinically wiping or sharp wound debridement. Our method is based on a variation of impression cytology in which a nitrocellulose membrane was used to collect surface biofilm components, which were then differentially stained. In this prospective study, members of an interdisciplinary pressure ulcer team at a university hospital tested our method's ability to predict the generation of wound slough in the week that followed each blotting. A total of 70 blots collected from 23 pressure ulcers produced 27 wounds negative for staining and 43 positive. In the negative blots 55.6% were found to have decreased wound slough, while 81.4% with positive staining had either increase or unchanged wound slough generation. These results lead to an odds ratio of positive blotting cases of 9.37 (95% confidence intervals: 2.47-35.5, p = 0.001) for slough formation; suggesting that the changes in wound slough formation can be predicted clinically using a non-invasive wound blotting method.

  18. Use of a Combined Duplex PCR/Dot Blot Assay for more sensitive genetic characterization

    Directory of Open Access Journals (Sweden)

    Erin Curry

    2008-01-01

    Full Text Available A reliable and sensitive method of genetic analysis is necessary to detect multiple specific nucleic acid sequences from samples containing limited template. The most widely utilized method of specific gene detection, polymerase chain reaction (PCR, imparts inconsistent results when assessing samples with restricted template, especially in a multiplex reaction when copies of target genes are unequal. This study aimed to compare two methods of PCR product analysis, fluorescent detection following agarose gel electrophoresis or dot blot hybridization with chemiluminescent evaluation, in the detection of a single copy gene (SRY and a multicopy gene (β-actin. Bovine embryo sex determination was employed to exploit the limited DNA template available and the target genes of unequal copies. Primers were used either independently or together in a duplex reaction with purified bovine genomic DNA or DNA isolated from embryos. When used independently, SRY and β-actin products were detected on a gel at the equivalent of 4-cell or 1-cell of DNA, respectively; however, the duplex reaction produced visible SRY bands at the 256 cell DNA equivalent and β-actin products at the 64 cell DNA equivalent. Upon blotting and hybridization of the duplex PCR reaction, product was visible at the 1–4 cell DNA equivalent. Duplex PCR was also conducted on 186 bovine embryos and product was subjected to gel electrophoresis or dot-blot hybridization in duplicate. Using PCR alone, sex determination was not possible for 22.6% of the samples. Using PCR combined with dot blot hybridization, 100.0% of the samples exhibited either both the male specific and β-actin products or the β-actin signal alone, indicating that the reaction worked in all samples. This study demonstrated that PCR amplification followed by dot blot hybridization provided more conclusive results in the evaluation of samples with low DNA concentrations and target genes of unequal copies.

  19. Design, preparation and use of ligated phosphoproteins: a novel approach to study protein phosphatases by dot blot array, ELISA and Western blot assays.

    Science.gov (United States)

    Sun, Luo; Ghosh, Inca; Barshevsky, Tanya; Kochinyan, Samvel; Xu, Ming-Qun

    2007-07-01

    The study of substrate specificity of protein phosphatases (PPs) is very challenging since it is difficult to prepare a suitable phosphorylated substrate. Phosphoproteins, phosphorylated by a protein kinase, or chemically synthesized phosphopeptides are commonly used substrates for PPs. Both types of these substrates have their advantages and limitations. Phosphoproteins mimic more closely the physiologically relevant PP substrates, but their preparation is technically demanding. Synthetic phosphopeptides present advantages over proteins because they can be easily produced in large quantity and their amino acid sequence can be designed to contain potential determinants of substrate specificity. However, short peptides are less optimal compared to in vivo PP substrates and often display poor and variable binding to different matrices, resulting in low sensitivity in analysis of PP activity on solid support. In this work we utilize the intein-mediated protein ligation (IPL) technique to generate substrates for PPs, combining the advantages of proteins and synthetic peptides in one molecule. The ligation of a synthetic phosphopeptide to an intein-generated carrier protein (CP) with a one-to-one stoichiometry results in the formation of a ligated phosphoprotein (LPP). Three widely used assays, dot blot array, Western blot and ELISA were employed to study the PP activity on LPP substrates. Dephosphorylation was measured by detection of the remaining phosphorylation, or lack of it, with a phospho-specific antibody. The data show the advantage of LPPs over free peptides in assays on solid supports. LPPs exhibited enhanced binding to the matrices used in the study, which significantly improved sensitivity and consistency of the assays. In addition, saturation of the signal was circumvented by serial dilution of the assay samples. This report describes detailed experimental procedures for preparation of LPP substrates and their use in PP assays based on immobilization on

  20. DISKRIMINASI KELAMIN PADA IKAN TUNA SIRIP KUNING, Yellowfin tuna MENGGUNAKAN ANALISIS DOT BLOT DAN ELISA

    Directory of Open Access Journals (Sweden)

    Gusti Ngurah Permana

    2014-08-01

    Full Text Available Pemahaman tentang penentuan jenis kelamin dalam populasi induk merupakan hal yang sangat penting bagi keberhasilan program pembenihan. Pengukuran reaksi antibodi dan aktivitas hormon testosterone, serta estradiol adalah metode dengan potensi yang secara akurat dapat menentukan jenis kelamin ikan tanpa mematikan ikan. Tujuan penelitian ini adalah untuk mengetahui akurasi metode dot blot dan ELISA dengan 11-ketotestorsterone (11-KT yang tersedia secara komersial EIA-kit untuk membedakan jenis kelamin ikan tuna sirip kuning. Hasil analisis menunjukkan bahwa metode dot blot menghasilkan ekspresi vitelogenin tampak jelas pada individu betina dan efek plasma terlihat transparan, jika dibandingkan dengan individu jantan. Interpretasi dari metode ini memerlukan pengalaman dan keahlian dalam akurasi pembacaan hasil. Aktivitas hormon 11-KT dengan sampel klip sirip dan plasma memberikan hasil yang baik dengan aktivitas hormon terlihat jelas.

  1. Western blot diagnosis of vivax malaria with multiple stage-specific antigens of the parasite

    OpenAIRE

    Son, Eui-Sun; Kim, Tong Soo; Nam, Ho-Woo

    2001-01-01

    Western blot analysis was performed to diagnose vivax malaria using stage-specific recombinant antigens. Genomic DNA from the whole blood of a malaria patient was used as templates to amplify the coding regions for the antigenic domains of circumsporozoite protein (CSP-1), merozoite surface protein (MSP-1), apical merozoite antigen (AMA-1), serine repeat antigen (SERA), and exported antigen (EXP-1) of Plasmodium vivax. Each amplified DNA fragment was inserted into a pGEX-4T plasmid to induce ...

  2. Positive IgG Western Blot for Borrelia burgdorferi in Colombia

    Directory of Open Access Journals (Sweden)

    Palacios Ricardo

    1999-01-01

    Full Text Available In order to evaluate the presence of specific IgG antibodies to Borrelia burgdorferi in patients with clinical manifestations associated with Lyme borreliosis in Cali, Colombia, 20 serum samples from patients with dermatologic signs, one cerebrospinal fluid (CSF sample from a patient with chronic neurologic and arthritic manifestations, and twelve serum samples from individuals without clinical signs associated with Lyme borreliosis were analyzed by IgG Western blot. The results were interpreted following the recommendations of the Centers for Diseases Control and Prevention (CDC for IgG Western blots. Four samples fulfilled the CDC criteria: two serum specimens from patients with morphea (localized scleroderma, the CSF from the patient with neurologic and arthritic manifestations, and one of the controls. Interpretation of positive serology for Lyme disease in non-endemic countries must be cautious. However these results suggest that the putative "Lyme-like" disease may correlate with positivity on Western blots, thus raising the possibility that a spirochete genospecies distinct from B. burgdorferi sensu stricto, or a Borrelia species other than B. burgdorferi sensu lato is the causative agent. Future work will focus on a survey of the local tick and rodent population for evidence of spirochete species that could be incriminated as the etiologic agent.

  3. Banding pattern indicative of echinococcosis in a commercial cysticercosis western blot

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    Tappe D

    2009-09-01

    Full Text Available Abstract Objective A commercial cysticercosis Western blot was evaluated for serological cross-reactivity of sera from patients with alveolar (AE and cystic echinococcosis (CE. Methods A total of 161 sera were examined, including 31 sera from AE-patients, 11 sera from CE-patients, 9 sera from patients with other parasitic diseases and 109 sera from patients with unrelated medical conditions. All AE-and CE-sera were also examined by the echinococcosis Western blot. Results More sera from patients with AE than with CE showed cross-reactivity in the form of ladder-like patterns ("Mikado aspect" and untypical bands at 6-8 kDa (71% and 77.4% versus 27.3% and 45.5%, respectively. In contrast, triplets of bands in the area above 50 kDa and between 24 and 39-42 kDa were more frequent in CE than in AE sera. The fuzzy band at 50-55 kDa typical for cysticercosis was absent in all AE and CE sera. Conclusions Atypical banding patterns in the cysticercosis Western blot should raise the suspicion of a metacestode infection different from Taenia solium, i.e. Echinococcus multilocularis or E. granulosus, especially when the Mikado aspect and an altered 6-8 kDa band is visible in the absence of a fuzzy 50-55 kDa band.

  4. Standardization of Licorice and TCM Formulations Using Eastern Blot Fingerprinting Analysis

    Directory of Open Access Journals (Sweden)

    Yukihiro Shoyama

    2013-01-01

    Full Text Available To prepare the antiglycyrrhizin (GC monoclonal antibody (MAb, GC was treated with NaIO4 resulting in aldehyde which can be combined with carrier protein. An antigen conjugate was performed by a matrix-assisted laser desorption/ionization TOF mass spectrometry to determine the hapten numbers in the conjugate. Anti-GC MAb was prepared from a hybridoma which was fixed from the spleen cells producing anti-GC MAb and the myeloma cells after immunization. The TCM and licorice extract were developed by TLC and blotted to a polyvinylidene difluoride (PVDF membrane. The membrane was treated by NaIO4 and protein, enzyme labeled secondary MAb, and finally substrate was added. Clear spot appeared on PVDF membrane identifying GC against a background containing large amount of impurities. In eastern blotting, the GC molecule was divided into two functions. The aglycone part is recognized as an epitope and the sugar moiety can be combined to membrane. The specific reactivity of sugar moiety in the GC molecule against anti-GC MAb might be modified by the NaIO4 treatment on the membrane because glycyrrhetic acid 3-O-glucuronide can be stained although the cross-reactivity is only 4.3%. Eastern blotting for GC can not only apply for the standardization of licorice and TCM, but also it can open for the other bioactive products.

  5. DIFFERENTIATION OF PSEUDOCONDYLOMA OF VULVA AND CONDYLOMA ACUMINATA BY DOT BLOT HYBRIDIZATION AND POLYMERASE CHAIN REACTION

    Institute of Scientific and Technical Information of China (English)

    刘跃华; 王家璧; 司静懿

    1996-01-01

    This study differentiated pseudocondyloma of vulva from condyloma acunainata using dot blot hybridization and polymerase chain reaction (PCR). A total of 27 cases o{ pseudocondyloma of vulva and 65 cases of condyloma acuminata were selected for the sttldy. The genital lesions were examined clinically and were biopsled. Each biopsy v-as subjected to histological examination and HPV DNA analysis by dot blot hybridization and PCR. Dot blot analysis detected HPV DNA in 19(82.6%) out of 23 cases of condyloma acuminata and 2(25%) out of 8 cases pseudocondyloma of vulvae(P<0. 05). PCR detected HPV DNA in 51(92.7%) our of 55 cases of eondyloma acuminata, compared with none in 23 cases of pseudocondylorna(P<0. 001). HPV DNA was present in the majority of condyloma acuminata specimens, HPV 6 and 11 were the predominant types. Peudocondyloma is probably not associated with HPV. PCR was the most sensitive and useful techntque for HPV DNA detection.

  6. Use of a Western blot technique for the serodiagnosis of glanders

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    de Souza Marcilia MA

    2011-01-01

    Full Text Available Abstract Background The in vivo diagnosis of glanders relies on the highly sensitive complement fixation test (CFT. Frequently observed false positive results are troublesome for veterinary authorities and cause financial losses to animal owners. Consequently, there is an urgent need to develop a test with high specificity. Hence, a Western blot assay making use of a partly purified lipopolysaccaride (LPS containing antigen of three Burkholderia mallei strains was developed. The test was validated investigating a comprehensive set of positive and negative sera obtained from horses and mules from endemic and non endemic areas. Results The developed Western blot assay showed a markedly higher diagnostic specificity when compared to the prescribed CFT and therefore can be used as a confirmatory test. However, the CFT remains the test of choice for routine testing of glanders due to its high sensitivity, its feasibility using standard laboratory equipment and its worldwide distribution in diagnostic laboratories. Conclusions The CFT should be amended by the newly validated Western blot to increase the positive likelihood ratio of glanders serodiagnosis in non endemic areas or areas with low glanders prevalence. Its use for international trade of horses and mules should be implemented by the OIE.

  7. Proteínas inmunodominantes de Brucella Melitensis evaluadas por Western Blot

    Directory of Open Access Journals (Sweden)

    Elizabeth Anaya

    1997-01-01

    Full Text Available Se separaron extractos de proteínas totales de Brucella melitensis en gel 15% SDS-PAGE. Su seroreactividad fue analizada por Western Blot con resultados satisfactorios. Para éste propósito sueros controles negativos (n=03, sueros de pacientes con brucelosis (n=34, cólera (n=12, tifoidea (n=02 y tuberculosis (n=02 fueron usados. Esta prueba inmunodiagnóstica detectó bandas seroreactivas altamente específicas (100% correspondientes a 8,14,18, un complejo de 25-48 y 58kDa. La sensibilidad del test fue del 90% usando los sueros antes mencionados.

  8. A sensitive non-radioactive northern blot method to detect small RNAs.

    Science.gov (United States)

    Kim, Sang Woo; Li, Zhihua; Moore, Patrick S; Monaghan, A Paula; Chang, Yuan; Nichols, Mark; John, Bino

    2010-04-01

    The continuing discoveries of potentially active small RNAs at an unprecedented rate using high-throughput sequencing have raised the need for methods that can reliably detect and quantitate the expression levels of small RNAs. Currently, northern blot is the most widely used method for validating small RNAs that are identified by methods such as high-throughput sequencing. We describe a new northern blot-based protocol (LED) for small RNA (approximately 15-40 bases) detection using digoxigenin (DIG)-labeled oligonucleotide probes containing locked nucleic acids (LNA) and 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide for cross-linking the RNA to the membrane. LED generates clearly visible signals for RNA amounts as low as 0.05 fmol. This method requires as little as a few seconds of membrane exposure to outperform the signal intensity using overnight exposure of isotope-based methods, corresponding to approximately 1000-fold improvement in exposure-time. In contrast to commonly used radioisotope-based methods, which require freshly prepared and hazardous probes, LED probes can be stored for at least 6 months, facilitate faster and more cost-effective experiments, and are more environmentally friendly. A detailed protocol of LED is provided in the Supplementary Data.

  9. Recombinant antigen-based immuno-slot blot method for serodiagnosis of syphilis

    Directory of Open Access Journals (Sweden)

    N.S. Sato

    2004-07-01

    Full Text Available Three recombinant antigens of Treponema pallidum Nichols strain were fused with GST, cloned and expressed in Escherichia coli, resulting in high levels of GST-rTp47 and GST-rTp17 expression, and supplementation with arginine tRNA for the AGR codon was needed to obtain GST-rTp15 overexpression. Purified fusion protein yields were 1.9, 1.7 and 5.3 mg/l of cell culture for GST-rTp47, GST-rTp17 and GST-rTp15, respectively. The identities of the antigens obtained were confirmed by automated DNA sequencing using ABI Prism 310 and peptide mapping by Finningan LC/MS. These recombinant antigens were evaluated by immuno-slot blot techniques applied to 137 serum samples from patients with a clinical and laboratory diagnosis of syphilis (61 samples, from healthy blood donors (50 samples, individuals with sexually transmitted disease other than syphilis (3 samples, and from individuals with other spirochetal diseases such as Lyme disease (20 samples and leptospirosis (3 samples. The assay had sensitivity of 95.1% (95% CI, 86.1 to 98.7% and a specificity of 94.7% (95% CI, 87.0 to 98.7%; a stronger reactivity was observed with fraction rTp17. The immunoreactivity results showed that fusion recombinant antigens based-immuno-slot blot techniques are suitable for use in diagnostic assays for syphilis.

  10. Simultaneous preparation of RNA and nuclei for Northern blot and flow cytometric analysis

    Energy Technology Data Exchange (ETDEWEB)

    Tesfaigzi, J.; Jaramillo, R. [Inhalation Toxicology Research Inst., Albuquerque, NM (United States)

    1996-11-01

    Several methods have been developed to quantify RNA synthesis during the progression of the cell cycle. The rate of RNA synthesis can be detected during different stages of the cell cycle by staining cells with agents that intercalate with nucleic acids. For example, following staining of mammalian cells with acridin orange, the green and red fluorescence that correlates with DNA and RNA content, respectively, can be analyzed by flow cytometry. Increase in RNA content during the progression of cells through the cell cycle can be measured after staining with acridin orange. RNA synthesis resulting from the stimulation of quiescent cells with various growth factors has also been demonstrated by labeling cells with bromo-uridine and using the anti-bromo-deoxyuridine antibody. These methods allow measurement of the overall RNA content in cells; however, they do not allow the measurement of the levels of specific mRNAs throughout the cell cycle. Current methods to quantify specific mRNAs generally require the preparation of a large number of cells (5--10 {times} 10{sup 6} cells) to carry out flow cytometric analyses and to isolate RNA for Northern blot analysis or solution hybridization. In this report, the authors describe a method of simultaneously preparing RNA and nuclei for Northern blot and flow cytometric analyses, respectively. The minimum number of nuclei required to obtain flow cytometric data and the effect of conserving nuclei in methanol for several days are also presented.

  11. Gel electrophoresis of polyphenol oxidase with instant identification by in situ blotting.

    Science.gov (United States)

    Cheng, Tsai-Mu; Huang, Pei-Chen; Pan, Ju-Pin; Lin, Kuan-Yu; Mao, Simon J T

    2007-04-15

    Polyphenol oxidase (PPO) or tyrosinase is an important and ubiquitous enzyme responsible for browning in plants and melanization in animals. The molecular size of the plant PPO is varied among the species and its activity can be enhanced by a variety of anionic detergents. In the present study, we developed a simple method for the first-step identification of PPO in fruit and vegetable extracts. First, 3mm chromatographic paper was immersed in 0.5% (w/v) catechol solution as an immobilized PPO substrate. After running the extract with 10% sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE), one side of the glass plate was removed. The plate was immediately laid on top of the dried catechol-paper. A dark-brown band corresponding to PPO was visualized within 1 min and was further confirmed by a conventional Western blot using an antibody prepared against mushroom PPO. It also reveals that some vegetation (such as tomato, radish, and oriental melon) with low or no detectable activity in a conventional enzyme assay actually possessed marked levels of PPO activity when assessed by PAGE-blot. We propose that an inhibitor is associated with PPO in some plants; the inhibitor, however, is dissociated during the electrophoresis. Therefore, in addition to identify the molecular form of PPO, the present technique may explore the existence of PPO inhibitor(s) in plants. The detail of the method with respect to its relevance for searching a natural PPO inhibitor is described and discussed.

  12. Application of a Dot Blot Hybridization Platform to Assess Streptococcus uberis Population Structure in Dairy Herds

    Science.gov (United States)

    Albuquerque, Pedro; Ribeiro, Niza; Almeida, Alexandre; Panschin, Irena; Porfirio, Afonso; Vales, Marta; Diniz, Francisca; Madeira, Helena; Tavares, Fernando

    2017-01-01

    Streptococcus uberis is considered one of the most important pathogens associated with bovine mastitis. While traditionally acknowledged as an environmental pathogen, S. uberis has been shown to adopt a contagious epidemiological pattern in several dairy herds. Since different control strategies are employed depending on the mode of transmission, in-depth studies of S. uberis populations are essential to determine the best practices to control this pathogen. In this work, we optimized and validated a dot blot platform, combined with automatic image analysis, to rapidly assess the population structure of infective S. uberis, and evaluated its efficiency when compared to multilocus sequence analysis (MLSA) genotyping. Two dairy herds with prevalent S. uberis infections were followed in a 6 month period, in order to collect and characterize isolates from cows with persistent infections. These herds, located in Portugal (Barcelos and Maia regions), had similar management practices, with the herd from Barcelos being smaller and having a better milking parlor management, since infected cow segregation was immediate. A total of 54 S. uberis isolates were obtained from 24 different cows from the two herds. To overcome operator-dependent analysis of the dot blots and increase the technique's consistency and reliability, the hybridization signals were converted into probability values, with average probabilities higher than 0.5 being considered positive results. These data allowed to confirm the isolates' identity as S. uberis using taxa-specific markers and to determine the presence of virulence- and antibiotic resistance-related genes. In addition, MLSA allowed to disclose the most prevalent S. uberis clonal lineages in both herds. Seven different clusters were identified, with Barcelos showing a high clonal diversity and Maia a dominant lineage infecting most cows, suggesting distinct epidemiological patterns, with S. uberis displaying an environmental or contagious

  13. Western Blot Detection of Human Anti-Chikungunya Virus Antibody with Recombinant Envelope 2 Protein

    Science.gov (United States)

    Yang, Zhaoshou; Lee, Jihoo; Ahn, Hye-Jin; Chong, Chom-Kyu; Dias, Ronaldo F.; Nam, Ho-Woo

    2016-01-01

    Chikungunya virus (CHIKV), a tropical pathogen, has re-emerged and has massive outbreaks abruptly all over the world. Containing many dominant epitopes, the envelope E2 protein of CHIKV has been explored for the vaccination or diagnosis. In the present study, the antigenicity of a recombinant expressed intrinsically disorder domain (IUD) of E2 was tested for the detection of the antibody against CHIKV through western blot method. The gene of the IUD of E2 was inserted into 2 different vectors and expressed as recombinant GST-E2 and recombinant MBP-E2 fusion protein, respectively. Two kinds of fusion proteins were tested with 30 CHIKV patient sera and 30 normal sera, respectively. Both proteins were detected by 25 patients sera (83.3%) and 1 normal serum (3.3%). This test showed a relatively high sensitivity and very high specificity of the recombinant E2 proteins to be used as diagnostic antigens against CHIKV infection. PMID:27180586

  14. Analysis of sperm membrane antigens relevant to antisperm antibody using Western blot

    Institute of Scientific and Technical Information of China (English)

    WangHF

    2002-01-01

    Objective:To identify the sperm membrane antigens associated with antisperm antibody.Methods:The antisperm antibody in serum was tested by ELISA.Antisperm antibody positive sera from 18 infertile men and 15 infertile women were used.The molecular weight(MW) of sperm membrane antigens associated with the antisperm antibody was analyzed with antisperm antibody positive serum using Western blot.Results:Eight kinds of MW of sperm membrane antigens were identified.The ratio of identification on the 78 KD(60.7%),60KD(71.4%),51KD(14.9%) and 23KD(14.29%)sperm antigen was higher than other.Conclusion:sperm membrane antigens with MW of 78KD,60KD,51KD and 23KD were associated with antisperm antibody and immunological infertility.

  15. Electrospun nitrocellulose and nylon: Design and fabrication of novel high performance platforms for protein blotting applications

    Directory of Open Access Journals (Sweden)

    Bowlin Gary L

    2007-10-01

    Full Text Available Abstract Background Electrospinning is a non-mechanical processing strategy that can be used to process a variety of native and synthetic polymers into highly porous materials composed of nano-scale to micron-scale diameter fibers. By nature, electrospun materials exhibit an extensive surface area and highly interconnected pore spaces. In this study we adopted a biological engineering approach to ask how the specific unique advantages of the electrospinning process might be exploited to produce a new class of research/diagnostic tools. Methods The electrospinning properties of nitrocellulose, charged nylon and blends of these materials are characterized. Results Nitrocellulose electrospun from a starting concentration of Conclusion The flexibility afforded by electrospinning process makes it possible to tailor blotting membranes to specific applications. Electrospinning has a variety of potential applications in the clinical diagnostic field of use.

  16. Rapid detection and differentiation of important Campylobacter spp. in poultry samples by dot blot and PCR.

    Science.gov (United States)

    Fontanot, Marco; Iacumin, Lucilla; Cecchini, Francesca; Comi, Giuseppe; Manzano, Marisa

    2014-10-01

    The detection of Campylobacter, the most commonly reported cause of foodborne gastroenteritis in the European Union, is very important for human health. The most commonly recognised risk factor for infection is the handling and/or consumption of undercooked poultry meat. The methods typically applied to evaluate the presence/absence of Campylobacter in food samples are direct plating and/or enrichment culture based on the Horizontal Method for Detection and Enumeration of Campylobacter spp. (ISO 10272-1B: 2006) and PCR. Molecular methods also allow for the detection of cells that are viable but cannot be cultivated on agar media and that decrease the time required for species identification. The current study proposes the use of two molecular methods for species identification: dot blot and PCR. The dot blot method had a sensitivity of 25 ng for detection of DNA extracted from a pure culture using a digoxigenin-labelled probe for hybridisation; the target DNA was extracted from the enrichment broth at 24 h. PCR was performed using a pair of sensitive and specific primers for the detection of Campylobacter jejuni and Campylobacter coli after 24 h of enrichment in Preston broth. The initial samples were contaminated by 5 × 10 C. jejuni cells/g and 1.5 × 10(2)C. coli cells/g, thus the number of cells present in the enrichment broth at 0 h was 1 or 3 cell/g, respectively.

  17. Western blot diagnosis of vivax malaria with multiple stage-specific antigens of the parasite.

    Science.gov (United States)

    Son, E S; Kim, T S; Nam, H W

    2001-06-01

    Western blot analysis was performed to diagnose vivax malaria using stage-specific recombinant antigens. Genomic DNA from the whole blood of a malaria patient was used as templates to amplify the coding regions for the antigenic domains of circumsporozoite protein (CSP-1), merozoite surface protein (MSP-1), apical merozoite antigen (AMA-1), serine repeat antigen (SERA), and exported antigen (EXP-1) of Plasmodium vivax. Each amplified DNA fragment was inserted into a pGEX-4T plasmid to induce the expression of GST fusion protein in Escherichia coli by IPTG. The bacterial cell extracts were separated on 10% SDS-PAGE followed by western blot analysis with patient sera which was confirmed by blood smear examination. When applied with patient sera, 147 (91.9%) out of 160 vivax malaria, 12 (92.3%) out of 13 falciparum malaria, and all 9 vivax/falciparum mixed malaria reacted with at least one antigen, while no reactions occurred with 20 normal uninfected sera. In the case of vivax malaria, CSP-1 reacted with 128 (80.0%) sera, MSP-1 with 102 (63.8%), AMA-1 with 128 (80.0%), SERA with 115 (71.9%), and EXP-1 with 89 (55.6%), respectively. We obtained higher detection rates when using 5 antigens (91.9%) rather than using each antigen solely (55.6-80%), a combination of 2 (76.3-87.5%), 3 (85.6-90.6%), or 4 antigens (89.4-91.3%). This method can be applied to serological diagnosis, mass screening in endemic regions, or safety test in transfusion of prevalent vivax malaria.

  18. Total protein analysis as a reliable loading control for quantitative fluorescent Western blotting.

    Directory of Open Access Journals (Sweden)

    Samantha L Eaton

    Full Text Available Western blotting has been a key technique for determining the relative expression of proteins within complex biological samples since the first publications in 1979. Recent developments in sensitive fluorescent labels, with truly quantifiable linear ranges and greater limits of detection, have allowed biologists to probe tissue specific pathways and processes with higher resolution than ever before. However, the application of quantitative Western blotting (QWB to a range of healthy tissues and those from degenerative models has highlighted a problem with significant consequences for quantitative protein analysis: how can researchers conduct comparative expression analyses when many of the commonly used reference proteins (e.g. loading controls are differentially expressed? Here we demonstrate that common controls, including actin and tubulin, are differentially expressed in tissues from a wide range of animal models of neurodegeneration. We highlight the prevalence of such alterations through examination of published "-omics" data, and demonstrate similar responses in sensitive QWB experiments. For example, QWB analysis of spinal cord from a murine model of Spinal Muscular Atrophy using an Odyssey scanner revealed that beta-actin expression was decreased by 19.3±2% compared to healthy littermate controls. Thus, normalising QWB data to β-actin in these circumstances could result in 'skewing' of all data by ∼20%. We further demonstrate that differential expression of commonly used loading controls was not restricted to the nervous system, but was also detectable across multiple tissues, including bone, fat and internal organs. Moreover, expression of these "control" proteins was not consistent between different portions of the same tissue, highlighting the importance of careful and consistent tissue sampling for QWB experiments. Finally, having illustrated the problem of selecting appropriate single protein loading controls, we demonstrate

  19. Evaluation of the Western blotting method for the diagnosis of congenital toxoplasmosis,

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    Jaqueline Dario Capobiango

    Full Text Available Abstract Objective: To evaluate the Western blotting method for the detection of IgG anti-Toxoplasma gondii (T. gondii (IgG-WB in the serum of children with suspected congenital toxoplasmosis. Methods: We accompanied 47 mothers with acquired toxoplasmosis in pregnancy and their children, between June of 2011 and June of 2014. The IgG-WB was done in house and the test was considered positive if the child had antibodies that recognized at least one band on IgG blots different from the mother's or with greater intensity than the corresponding maternal band, during the first three months of life. Results: 15 children (15.1% met the criteria for congenital toxoplasmosis and 32 (32.3% had the diagnosis excluded. The symptoms were observed in 12 (80.0% children and the most frequent were cerebral calcification in 9 (60.0%, chorioretinitis in 8 (53.3%, and hydrocephalus in 4 (26.6%. IgM antibodies anti-T. gondii detected by chemiluminescence (CL were found in 6 (40.0% children and the polymerase chain reaction (PCR for detection of T. gondii DNA was positive in 5 of 7 performed (71.4%. The sensitivity of IgG-WB was of 60.0% [95% confidence interval (CI 32.3-83.7%] and specificity 43.7% (95% CI 26.7-62.3%. The sensitivity of IgG-WB increased to 76.0 and 89.1% when associated to the research of IgM anti-T. gondii or PCR, respectively. Conclusions: The IgG-WB showed greater sensitivity than the detection of IgM anti-T. gondii; therefore, it can be used for the diagnosis of congenital toxoplasmosis in association with other congenital infection markers.

  20. HIV‑2 antibody detection after indeterminate or negative HIV‑1 Western blot in Cuba, 2005-2008.

    Science.gov (United States)

    Díaz, Dervel F; Ortiz, Eva; Martín, Dayamí; Nibot, Carmen; Rizo, Adis; Silva, Eladio

    2012-01-01

    INTRODUCTION Differentiating between HIV-1 and HIV-2 infection is the first step to understanding HIV transmission, epidemiology and pathogenesis in geographical areas where both viruses circulate. In Cuba, positive results in mixed HIV-1/2 screening assays are confirmed by HIV-1 Western blot. Indeterminate results constitute the main limitation of this test and HIV-2 infection is among their possible causes; hence the importance of second-stage screening and confirmatory tests for HIV-2 infection. OBJECTIVE Investigate the contribution of HIV-2 antibodies to negative or indeterminate HIV-1 Western blot results in serum samples from 2005 through 2008 in Cuba. METHODS HIV-2 reactivity was studied using the ELISA DAVIH-VIH-2 diagnostic kit (Cuba) in 1723 serum samples with negative or indeterminate results for HIV-1 Western blot from January 2005 through December 2008. Duplicate sera reactive by ELISA were confirmed by HIV-2 Western blot, results interpreted according to WHO criteria. The epidemiological interview established by Cuba's National Program for Prevention and Control Sexually-Transmitted Diseases and HIV/AIDS was applied to HIV-2 Western blot-positive patients. RESULTS Among all sera studied, HIV-2 ELISA identified 12 reactive serum samples (0.70%) and 1711 non-reactive (99.30%). Western blot analysis of the 12 ELISA-reactive samples confirmed two positive samples (16.67%), 4 negative (33.33%) and 6 indeterminate (50%). Positive samples reacted against the p16, p26, gp36, p53, p56, p68 and gp105 proteins. All 12 ELISA-reactive samples belonged to the HIV-1 Western blot indeterminate group. The two HIV-2-positive samples showed well defined reactivity to gp160, p53, p55 and p34 of HIV-1. HIV-1 seroconversion was observed in all 10 remaining samples during serological followup. CONCLUSIONS Two new HIV-2 seropositive cases were diagnosed using DAVIH-VIH-2 and HIV-2 Western blot in indeterminate HIV-1 Western blot samples. Results support the recommendation

  1. Characterization of excretory-secretory antigens of adult Toxocara canis by western blotting.

    Science.gov (United States)

    Sudhakar, N R; Samanta, S; Sahu, Shivani; Raina, O K; Gupta, S C; Goswami, T K; Lokesh, K M; Kumar, Ashok

    2014-06-01

    Toxocara canis is one of the most common helminth worm of dogs which continues to stimulate both public health concern alongside the higher scientific interest. It may cause visceral and ocular damage in humans especially in children. The identification of specific antigens of T. canis is important so as to develop better diagnostic techniques. Excretory-secretory (ES) antigens were prepared by culturing the adult T. canis worms in RPMI 1640 medium without serum supplementation followed by ammonium sulphate precipitation. These antigens were separated using sodium dodecyl sulphate-electrophoresis (SDS-PAGE). Recovered proteins ranged from 30 to 384 kDa. The specific reactivity of the T. canis excretory-secretory (TC-ES) proteins was checked by western blotting. The immuno-reactivity of the naturally infected dog sera with the TC-ES antigens showed five bands at 43, 57,105, 139 and 175 kDa. The immuno-reactivity of the hyper immune serum raised in rabbits against TC-ES antigens was observed with ten polypeptides of 21, 25, 30, 37, 45, 50, 57, 69, 77 and 105 kDa. Common antigens band were observed at 57 and 105 KDa. These antigens merit further evaluation as candidate for use in diagnosis of toxocariasis in humans and adult dogs.

  2. The mouse prostaglandin E receptor EP2 subtype: cloning, expression, and northern blot analysis.

    Science.gov (United States)

    Katsuyama, M; Nishigaki, N; Sugimoto, Y; Morimoto, K; Negishi, M; Narumiya, S; Ichikawa, A

    1995-09-25

    A functional cDNA clone for the mouse prostaglandin (PG) E receptor EP2 subtype was isolated from a mouse cDNA library. The mouse EP2 receptor consists of 362 amino acid residues with seven putative transmembrane domains. [3H]PGE2 bound specifically to the membrane of Chinese hamster ovary cells stably expressing the cloned receptor. This binding was displaced by unlabeled prostanoids in the order of PGE2 = PGE1 > iloprost, a stable PGI2 agonist > PGF2 alpha > PGD2. Binding was also inhibited by butaprost (an EP2 agonist) and to a lesser extent by M&B 28767 (an EP3 agonist), but not by sulprostone (an EP1 and EP3 agonist) or SC-19220 (an EP1 antagonist). PGE2 and butaprost increased the cAMP level in the Chinese hamster ovary cells in a concentration-dependent manner. Northern blot analysis revealed that EP2 mRNA is expressed most abundantly in the uterus, followed by the spleen, lung, thymus, ileum, liver, and stomach.

  3. Rapid detection of intestinal pathogens in fecal samples by an improved reverse dot blot method

    Institute of Scientific and Technical Information of China (English)

    Jian-Ming Xing; Su Zhang; Ying Du; Dan Bi; Li-Hui Yao

    2009-01-01

    AIM:To develop a new, rapid and accurate reverse dot blot (RDB) method for the detection of intestinal pathogens in fecal samples.METHODS:The 12 intestinal pathogens tested were Salmonella spp., Brucella spp., Escherichia coli O157:H7,Clostridium botulinum, Bacillus cereus,Clostridium perfringens, Vibrio parahaemolyticus,Shigella spp., Yersinia enterocolitica, Vibrio cholerae,Listeria monocytogenes and Staphylococcus aureus.The two universal primers were designed to amplify two variable regions of bacterial 16S and 23S rDNA genes from all of the 12 bacterial species tested. Five hundred and forty fecal samples from the diarrhea patients were detected using the improved RDB assay.RESULTS:The methods could identify the 12 intestinal pathogens specifically, and the detection limit was as low as 103 CFUs. The consistent detection rate of the improved RDB assay compared with the traditional culture method was up to 88.75%.CONCLUSION:The hybridization results indicated that the improved RDB assay developed was a reliable method for the detection of intestinal pathogen in fecal samples.

  4. A simple dot-blot-Sirius red-based assay for collagen quantification.

    Science.gov (United States)

    Rodríguez-Rodríguez, Pilar; Arribas, Silvia M; de Pablo, Angel Luis López; González, M Carmen; Abderrahim, Fatima; Condezo-Hoyos, Luis

    2013-08-01

    The assessment of collagen content in tissues is important in biomedical research, since this protein is altered in numerous diseases. Hydroxyproline and Sirius red based assays are the most common methods for collagen quantification. However, these procedures have some pitfalls, such as the requirement of oxygen-free medium or expensive equipment and large sample size or being unsuitable for hydrolyzed collagen, respectively. Our objective was to develop a specific, versatile, and user-friendly quantitative method applicable to small tissue samples and extracts obtained from elastin purification, therefore, suitable for simultaneous quantification of elastin. This method is based on the binding of Sirius red to collagen present in a sample immobilized on a PVDF membrane, as in the dot-blot technique, and quantified by a scanner and image analysis software. Sample loading, Sirius red concentration, temperature and incubation time, type of standard substance, albumin interference, and quantification time are optimized. The method enabled the quantification of (1) intact collagen in several rat tissue homogenates, including small resistance-sized arteries, (2) partially hydrolyzed collagen obtained from NaOH extracts, compatible with elastin purification, and (3) for the detection of differences in collagen content between hypertensive and normotensive rats. We conclude that the developed technique can be widely used since it is versatile (quantifies intact and hydrolyzed collagen), requires small sample volumes, is user-friendly (low-cost, easy to use, minimum toxic materials, and reduced time of test), and is specific (minimal interference with serum albumin).

  5. Serological diagnosis of North American Paragonimiasis by Western blot using Paragonimus kellicotti adult worm antigen.

    Science.gov (United States)

    Fischer, Peter U; Curtis, Kurt C; Folk, Scott M; Wilkins, Patricia P; Marcos, Luis A; Weil, Gary J

    2013-06-01

    Abstract. We studied the value of an IgG Western blot (WB) with Paragonimus kellicotti (Pk) antigen for diagnosis of North American paragonimiasis. The test was evaluated with sera from patients with Pk and Paragonimus westermani infections, with control sera from patients with other helminth infections, and sera from healthy Americans. All 11 proven Pk infection sera and two samples from suspected cases that were negative by P. westermani WB at the Centers for Disease Control and Prevention (CDC) contained antibodies to antigens at 34 kDa and at 21/23 kDa. Seven of 7 P. westermani sera contained antibodies to the 34 kDa antigen, but only 2 recognized the 21/23 kDa doublet. No control samples were reactive with these antigens. Antibody reactivity declined after praziquantel treatment. Thus, the P. kellicotti WB appears to be superior to P. westermani WB for diagnosing Pk infections, and it may be useful for assessing responses to treatment.

  6. Binding of 16S rRNA to chloroplast 30S ribosomal proteins blotted on nitrocellulose.

    Science.gov (United States)

    Rozier, C; Mache, R

    1984-10-11

    Protein-RNA associations were studied by a method using proteins blotted on a nitrocellulose sheet. This method was assayed with Escherichia Coli 30S ribosomal components. In stringent conditions (300 mM NaCl or 20 degrees C) only 9 E. coli ribosomal proteins strongly bound to the 16S rRNA: S4, S5, S7, S9, S12, S13, S14, S19, S20. 8 of these proteins have been previously found to bind independently to the 16S rRNA. The same method was applied to determine protein-RNA interactions in spinach chloroplast 30S ribosomal subunits. A set of only 7 proteins was bound to chloroplast rRNA in stringent conditions: chloroplast S6, S10, S11, S14, S15, S17 and S22. They also bound to E. coli 16S rRNA. This set includes 4 chloroplast-synthesized proteins: S6, S11, S15 and S22. The core particles obtained after treatment by LiCl of chloroplast 30S ribosomal subunit contained 3 proteins (S6, S10 and S14) which are included in the set of 7 binding proteins. This set of proteins probably play a part in the early steps of the assembly of the chloroplast 30S ribosomal subunit.

  7. Renaturation of blotted allergens increases the sensitivity of specific IgE detection.

    Science.gov (United States)

    Muro, M D; Fernández, C; Moneo, I

    1996-01-01

    Several authors have demonstrated that renaturation is an essential step for the appropriate recognition of blotted proteins. The use of nonionic detergents has been described as a useful alternative to enhance the antigenicity in immunoblotting, although elution from proteins by detergents has been observed. To measure the influence of different factors on the sensitivity of specific IgE by immunoblotting, we used twenty human sera from atopic patients who were allergic or nonallergic to a common, reliable allergen (grass pollen mixture). The use of Nonidet-P40 was found to be a useful alternative for the renaturation of the allergens. No elution from the membrane was found when employing this detergent, even at high concentrations (3%), and its use gave better sensitivity than methanol. On the other hand, we detected that methanol possessed renaturing properties. A transfer method using diffusion instead of electric transfer gave the best results and two membranes could be obtained from each gel. Using this method, we found that after NP-40 incubation of the membrane, the use of bovine albumin could be omitted as blocking agent and that its use had even deleterious effects.

  8. NORTHERN BLOT ANALYSIS OF nm23 GENE EXPRESSION IN HUMAN LUNG CANCER

    Institute of Scientific and Technical Information of China (English)

    LIU Lun-xu; ZHOU Qing-hua; SHI Ying-kang; QIN Yang; SUN Zhi-lin; SUN Ze-fang

    1999-01-01

    Objective: To investigate the role of nm23 gene expression in human lung cancer. Methods: Forty human lung cancer tissues and 19 non-cancer pulmonary tissues were studied for their nm23-H1 and nm23-H2 mRNA expression with non-radioactive Northern blot hybridization. The correlation of nm23 mRNA expression with clinical features of lung cancer was analyzed. Results: The mRNA expression of nm23-H2 gene in poorly differentiated squamous cell carcinoma was significantly decreased compared to that in moderate-high differentiated squamous cell carcinoma. The mRNA expression of nm23-H1 and nm23-H2 gene in small cell lung cancer was significantly decreased compared to that in squamous cell carcinoma. No significant difference in nm23 mRNA expression was observed between lung cancer with and without lymph node metastasis, nor was there significant difference between tumor stage. Conclusion: The mRNA expression of nm23 gene is correlated with the degree of differentiation of lung cancer, but there is no evidence of metastasis suppression effect by nm23 gene.

  9. Glyceraldehyde-3-phosphate dehydrogenase: a universal internal control for Western blots in prokaryotic and eukaryotic cells.

    Science.gov (United States)

    Wu, Yonghong; Wu, Min; He, Guowei; Zhang, Xiao; Li, Weiguang; Gao, Yan; Li, Zhihui; Wang, Zhaoyan; Zhang, Chenggang

    2012-04-01

    In the current study, we examined the expression level of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) protein in a number of organisms and the stability of GAPDH under various conditions. Our results revealed that GAPDH is present in multiple Escherichia coli strains, the yeast strain GS115, Caenorhabditis elegans, rat PC12 cells, and both mouse and rat brain. Furthermore, GAPDH was stably expressed under different concentrations of inducer and at different times of induction in E. coli (BL21) cells and yeast GS115 cells. Stable expression of GAPDH protein was also observed in C.elegans and PC12 cells that were treated with different concentrations of paraquat or sodium sulfite, respectively. In addition, we were able to detect and identify the endogenous gapA protein in E.coli via immunoprecipitation and MALDI-TOF-MS analysis. Endogenous gapA protein and exogenously expressed (subcloned) GAPDH proteins were detected in E. coli BL21 but not for gapC. With the exception of gapC in E. coli, the various isoforms of GAPDH possessed enzymatic activity. Finally, sequence analysis revealed that the GAPDH proteins were 76% identical, with the exception of E. coli gapC. Taken together, our results indicate that GAPDH could be universally used as an internal control for the Western blot analysis of prokaryotic and eukaryotic samples.

  10. Rapid Detection of Rifampin-resistant Clinical Isolates of Mycobacterium tuberculosis by Reverse Dot Blot Hybridization

    Institute of Scientific and Technical Information of China (English)

    GUO Qian; WAN Kang Lin; YU Yan; ZHU Yan Ling; ZHAO Xiu Qin; LIU Zhi Guang; ZHANG Yuan Yuan; LI Gui Lian; WEI Jian Hao; WU Yi Mou

    2015-01-01

    Objective A PCR-reverse dot blot hybridization (RDBH) assay was developed for rapid detection of rpoB gene mutations in‘hot mutation region’ of Mycobacterium tuberculosis (M. tuberculosis). Methods 12 oligonucleotide probes based on the wild-type and mutant genotype rpoB sequences of M. tuberculosis were designed to screen the most frequent wild-type and mutant genotypes for diagnosing RIF resistance. 300 M. tuberculosis clinical isolates were detected by RDBH, conventional drug-susceptibility testing (DST) and DNA sequencing to evaluate the RDBH assay. Results The sensitivity and specificity of the RDBH assay were 91.2%(165/181) and 98.3%(117/119), respectively, as compared to DST. When compared with DNA sequencing, the accuracy, positive predictive value (PPV) and negative predictive value (NPV) of the RDBH assay were 97.7%(293/300), 98.2%(164/167), and 97.0%(129/133), respectively. Furthermore, the results indicated that the most common mutations were in codons 531 (48.6%), 526 (25.4%), 516 (8.8%), and 511 (6.6%), and the combinative mutation rate was 15 (8.3%). One and two strains of insertion and deletion were found among all strains, respectively. Conclusion Our findings demonstrate that the RDBH assay is a rapid, simple and sensitive method for diagnosing RIF-resistant tuberculosis.

  11. Evaluation of an enzyme-linked immunoelectrotransfer blot test for the confirmatory serodiagnosis of human toxocariasis

    Directory of Open Access Journals (Sweden)

    William H Roldán

    2009-05-01

    Full Text Available To improve the serodiagnosis of human toxocariasis, a sensitive and specific enzyme-linked immunoelectrotransfer blot (EITB-IgG test was developed and evaluated using Toxocara canislarvae excretory-secretory antigens for detecting anti-Toxocara IgG antibodies. The EITB-IgG profile of toxocariasis was characterized by comparing 27 sera from patients with toxocariasis, 110 sera from healthy subjects and 186 sera from patients with other helminth diseases (ascariasis, ancylostomiasis, trichuriasis, enterobiasis, strongyloidiasis, hymenolepiasis, diphyllobothriasis, taeniasis, cysticercosis, hydatidosis and fascioliasis. Antigenic bands of 24, 28, 30, 35, 56, 117, 136 and 152 kDa were predominantly recognized in sera from all patients with toxocariasis. However, only bands of 24-35 kDa were highly specific for Toxocara infection (98.3%, whereas other antigenic bands observed displayed cross-reactivity. Additionally, when the results of the EITB-IgG test were compared to those of the ELISA-IgG test, a 100% concordance was observed for positive results in human toxocariasis cases. The concordance for negative results between the two tests for healthy subjects and patients with other helminth diseases were 96.3% and 53.7%, respectively, showing that the EITB-IgG test has a higher specificity than ELISA. In conclusion, the EITB-IgG test is a very useful tool to confirm the serological diagnosis of human toxocariasis.

  12. Evaluation of an enzyme-linked immunoelectrotransfer blot test for the confirmatory serodiagnosis of human toxocariasis.

    Science.gov (United States)

    Roldán, William H; Espinoza, Yrma A

    2009-05-01

    To improve the serodiagnosis of human toxocariasis, a sensitive and specific enzyme-linked immunoelectrotransfer blot (EITB-IgG) test was developed and evaluated using Toxocara canislarvae excretory-secretory antigens for detecting anti-Toxocara IgG antibodies. The EITB-IgG profile of toxocariasis was characterized by comparing 27 sera from patients with toxocariasis, 110 sera from healthy subjects and 186 sera from patients with other helminth diseases (ascariasis, ancylostomiasis, trichuriasis, enterobiasis, strongyloidiasis, hymenolepiasis, diphyllobothriasis, taeniasis, cysticercosis, hydatidosis and fascioliasis). Antigenic bands of 24, 28, 30, 35, 56, 117, 136 and 152 kDa were predominantly recognized in sera from all patients with toxocariasis. However, only bands of 24-35 kDa were highly specific for Toxocara infection (98.3%), whereas other antigenic bands observed displayed cross-reactivity. Additionally, when the results of the EITB-IgG test were compared to those of the ELISA-IgG test, a 100% concordance was observed for positive results in human toxocariasis cases. The concordance for negative results between the two tests for healthy subjects and patients with other helminth diseases were 96.3% and 53.7%, respectively, showing that the EITB-IgG test has a higher specificity than ELISA. In conclusion, the EITB-IgG test is a very useful tool to confirm the serological diagnosis of human toxocariasis.

  13. Identification of toxocara canis antigens by Western blot in experimentally infected rabbits

    Directory of Open Access Journals (Sweden)

    MORALES Olga Lucía

    2002-01-01

    Full Text Available Toxocariasis is a frequent helminthiasis that can cause visceral and ocular damage in humans specially in children. The identification of specific antigens of Toxocara canis is important in order to develop better diagnostic techniques. Ten rabbits were infected orally with a dose of 5000 Toxocara canis embryonated eggs. Rabbits were bled periodically and an ELISA assay was performed to determine levels of specific Toxocara IgG antibodies. ELISA detected antibodies at day 15 after infection. Western blot (WB assay was performed using excretory/secretory antigens (E/S of T. canis second stage larvae. Different antigen concentrations were evaluated: 150, 200, 250 and 300 µg/mL. The concentration of 250 µg/mL was retained for analysis. Rabbit sera were diluted 1:100. Secondary antibody was used at a dilution of 1:1000. Results of WB indicated that in the first month after infection specific antibodies against the 200 KDa, 116 KDa, 92 KDa and 35 KDa antigens were detected; antibodies against the 92 KDa, 80 KDa, 66 KDa, 45 KDa, 31 KDa and 28 KDa antigens appeared later. All positive sera in the ELISA test were also positive in WB. Two antigen bands, 92 KDa and 35 KDa, were identified since the beginning and throughout the course of infection. These antigens merit further evaluation as candidates for use in diagnosis.

  14. Electrostatic protein immobilization using charged polyacrylamide gels and cationic detergent microfluidic Western blotting.

    Science.gov (United States)

    Kim, Dohyun; Karns, Kelly; Tia, Samuel Q; He, Mei; Herr, Amy E

    2012-03-06

    We report a novel protein immobilization matrix for fully integrated microfluidic Western blotting (WB). The electrostatic immobilization gel (EIG) enables immobilization of all proteins sized using cetyl trimethylammonium bromide polyacrylamide gel electrophoresis (CTAB-PAGE), for subsequent electrophoretic probing with detection affinity reagents (e.g., labeled antibodies). The "pan-analyte" capture strategy introduced here uses polyacrylamide gel grafted with concentrated point charges (zwitterionic macromolecules), in contrast to existing microfluidic WB strategies that rely on a sandwich immunoassay format for analyte immobilization and detection. Sandwich approaches limit analyte immobilization to capture of only a priori known targets. A charge interaction mechanism study supports the hypothesis that electrostatic interaction plays a major role in analyte immobilization on the EIG. We note that protein capture efficiency depends on both the concentration of copolymerized charges and ionic strength of the gel buffer. We demonstrate pan-analyte immobilization of sized CTAB-laden model proteins (protein G, ovalbumin, bovine serum albumin, β-galactosidase, lactoferrin) on the EIG with initial capture efficiencies ranging from 21 to 100%. Target proteins fixed on the EIG (protein G, lactoferrin) are detected using antibody probes with signal-to-noise ratios of 34 to 275. The approach advances protein immunoblotting performance through 200× reduction on sample consumption, 12× reduction in assay duration, and automated assay operation, compared to slab-gel WB. Using the microfluidic WB assay, assessment of lactoferrin in human tear fluid is demonstrated with a goal of advancing toward nonbiopsy-based diagnosis of Sjögren's Syndrome, an autoimmune disease.

  15. Evaluation of a Western Blot Test in an Outbreak of Acute Pulmonary Histoplasmosis

    Science.gov (United States)

    Pizzini, Claudia V.; Zancopé-Oliveira, Rosely M.; Reiss, Errol; Hajjeh, Rana; Kaufman, Leo; Peralta, José Mauro

    1999-01-01

    A western blot (WB) test was evaluated for detection of antibodies against native glycosylated and chemically deglycosylated M and H antigens of Histoplasma capsulatum in serum obtained from patients during the acute phase of pulmonary histoplasmosis that occurred during an outbreak. Of 275 serum samples tested by immunodiffusion and complement fixation (CF) samples from 40 patients affected during this outbreak and from 37 negative controls were tested by WB test. A group of patients whose sera were negative for CF antibodies and precipitins early in the acute stage of histoplasmosis but who all seroconverted during convalescence 6 weeks later were tested with the WB test. Antibodies against untreated H and M antigens were detected at a 1:100 dilution by WB test in 45% of the 20 acute-phase serum samples and in all 20 of the convalescent-phase specimens. The WB test’s sensitivity for acute-phase specimens increased to 90% (18 of 20 specimens) when H and M antigens were treated by periodate oxidation to inactivate susceptible carbohydrate epitopes. When native glycosylated antigens were used in the WB test, positive reactions were observed in negative control serum specimens (3 of 37 specimens; 8%) and in serum specimens obtained from asymptomatic persons screened as part of the outbreak investigation (13 of 20 specimens; 65%). These positive reactions were also attributed to glycosidic epitopes since the specificity of the WB test increased from 78 to 100% when periodate-treated H and M antigens were used. WB test with deglycosylated H and M antigens of histoplasmin provides a rapid, sensitive, and specific test to diagnose acute pulmonary histoplasmosis before precipitins can be detected. PMID:9874658

  16. Evaluation of the Aspergillus Western blot IgG kit for diagnosis of chronic aspergillosis.

    Science.gov (United States)

    Oliva, A; Flori, P; Hennequin, C; Dubus, J-C; Reynaud-Gaubert, M; Charpin, D; Vergnon, J M; Gay, P; Colly, A; Piarroux, R; Pelloux, H; Ranque, S

    2015-01-01

    Immunoprecipitin detection (IPD) is the current reference confirmatory technique for anti-Aspergillus antibody detection; however, the lack of standardization is a critical drawback of this assay. In this study, we evaluated the performance of the Aspergillus Western blot (Asp-WB) IgG kit (LDBio Diagnostics, Lyon, France), a recently commercialized immunoblot assay for the diagnosis of various clinical presentations of chronic aspergillosis. Three hundred eight serum samples from 158 patients with aspergillosis sensu lato (s.l.) were analyzed. More specifically, 267 serum samples were derived from patients with Aspergillus disease, including 89 cases of chronic pulmonary aspergillosis, 10 of aspergilloma, and 32 of allergic bronchopulmonary aspergillosis, while 41 samples were from patients with Aspergillus colonization, including 15 cystic fibrosis (CF) and 12 non-CF patients. For blood donor controls, the Asp-WB specificity was 94%, while the kit displayed a sensitivity for the aspergillosis s.l. diagnosis of 88.6%, with a diagnostic odds ratio (DOR) of 119 (95% confidence interval [CI], 57 to 251). The DOR values were 185.22 (95% CI,78.79 to 435.45) and 43.74 (95% CI, 15.65 to 122.20) for the diagnosis of Aspergillus disease and Aspergillus colonization, respectively. Among the patients, the sensitivities of the Asp-WB in the diagnosis of Aspergillus colonization were 100% and 41.7% in CF and non-CF patients, respectively. The Asp-WB yielded fewer false-negative results than did IPD. In conclusion, the Asp-WB kit performed well for the diagnosis of various clinical presentations of aspergillosis in nonimmunocompromised patients, with an enhanced standardization and a higher sensitivity than with IPD, which is the current reference method.

  17. Evaluation of immunodominant proteins of Mycobacterium avium paratuberculosis cell wall by Western blot analysis.

    Science.gov (United States)

    Hashemi, Maryam; Madani, Rasool; Razmi, Nematollah

    2014-04-01

    Mycobacterium avium subspecies paratuberculosis (M. paratuberculosis) is a slow growing mycobactin, whose dependence on mycobacterial species is known to be the causative agent of Johne's disease (paratuberculosis) in all species of domestic ruminants worldwide. The organism is transmitted via close contact, ingestion, or transplacentally from mother to fetus and occurs commonly in grazing domestic animals. Johne's disease (JD) is characterized by gradual weight loss, decreased milk production, and diarrhea due to the chronic, progressive, granulomatous enteritis and lymphadenitis. The disease can cause serious economic damage to the dairy industry due to the loss of milk production and early culling of infected animals. In recent years, researchers have focused on the identification of a specific antigen of M. paratuberculosis to use in diagnosis test and preparation of effective vaccine. The goal of this study is evaluation of the immunodominant proteins of M. paratuberculosis cell wall. The amount of protein was determined with a Lowry assay (22.68 μg/100 μL). For production of polyclonal antibody against proteins of M. paratuberculosis cell wall, a New Zealand white rabbit was immunized with antigen and Freund's adjuvant. After immunization, the rabbit was bled to produce enriched serum. Antibodies were purified from serum with ion exchange chromatography. In the Ouchterlony test, the reactions between antigen and antibodies were seen in dilutions of one quarter for serum, one quarter for Ig, and one half for IgG by clear precipitation lines due to the well immunization of the rabbit. Electrophoresis and Western blot analysis were used and subsequently a sharp band appeared in nitrocellulose paper; these bands were about 25, 37, 50, 75, and 150 kDa molecular weight, which indicated immunodominant proteins.

  18. Detection of classical and atypical/Nor98 scrapie by the paraffin-embedded tissue blot method.

    Science.gov (United States)

    Wemheuer, W M; Benestad, S L; Wrede, A; Wemheuer, W E; Brenig, B; Bratberg, B; Schulz-Schaeffer, W J

    2009-05-30

    The paraffin-embedded tissue (PET) blot method was used to investigate sections of the central nervous system and lymphatic tissues from 24 cases of classical scrapie and 25 cases of atypical/Nor98 scrapie in sheep and four healthy control sheep. The PET blot detected deposits of PrP(Sc) in the brain tissue of all 49 sheep with scrapie but no PrP(Sc) labelling could be detected in the control sheep. By contrast, not all the atypical/Nor98 scrapie cases were detectable by immunohistochemistry. The high sensitivity of the PET blot method made it possible to observe that in some atypical/Nor98 cases, deposits of PrP(Sc) may be restricted to supratentorial brain structures and that the diagnosis may be missed when only testing the obex area, where deposits are common in classical scrapie, and the cerebellar structures, where deposits are considered to be common in atypical/Nor98 cases.

  19. A slot blot immunoassay for quantitative detection of Plasmodium falciparum circumsporozoite protein in mosquito midgut oocyst.

    Directory of Open Access Journals (Sweden)

    Sanjai Kumar

    Full Text Available There is still a need for sensitive and reproducible immunoassays for quantitative detection of malarial antigens in preclinical and clinical phases of vaccine development and in epidemiology and surveillance studies, particularly in the vector host. Here we report the results of sensitivity and reproducibility studies for a research-grade, quantitative enhanced chemiluminescent-based slot blot assay (ECL-SB for detection of both recombinant Plasmodium falciparum circumsporozoite protein (rPfCSP and native PfCSP from Oocysts (Pf Oocyst developing in the midguts of Anopheles stephensi mosquitoes. The ECL-SB detects as little as 1.25 pg of rPfCSP (linear range of quantitation 2.5-20 pg; R2 = 0.9505. We also find the earliest detectable expression of native PfCSP in Pf Oocyst by ECL-SB occurs on day 7 post feeding with infected blood meal. The ECL-SB was able to detect approximately as few as 0.5 day 8 Pf Oocysts (linear quantitation range 1-4, R2 = 0.9795 and determined that one Pf Oocyst expressed approximately 2.0 pg (0.5-3 pg of native PfCSP, suggesting a similar range of detection for recombinant and native forms of Pf CSP. The ECL-SB is highly reproducible; the Coefficient of Variation (CV for inter-assay variability for rPf CSP and native PfCSP were 1.74% and 1.32%, respectively. The CVs for intra-assay variability performed on three days for rPf CSP were 2.41%, 0.82% and 2% and for native Pf CSP 1.52%, 0.57%, and 1.86%, respectively. In addition, the ECL-SB was comparable to microscopy in determining the P. falciparum prevalence in mosquito populations that distinctly contained either high and low midgut Pf Oocyst burden. In whole mosquito samples, estimations of positivity for P. falciparum in the high and low burden groups were 83.3% and 23.3% by ECL-SB and 85.7% and 27.6% by microscopy. Based on its performance characteristics, ECL-SB could be valuable in vaccine development and to measure the parasite prevalence in mosquitoes

  20. Establishment of Southern blot to diagnose the Huntington disease%Huntington舞蹈病Southern杂交诊断方法的建立

    Institute of Scientific and Technical Information of China (English)

    马明义; 李华; 杜亭; 雷娟; 覃维; 张宇翔

    2013-01-01

    目的 建立诊断Huntington舞蹈病的Southern杂交方法.方法 PCR法扩增-497 bp的片段,克隆测序验证后再从质粒上切下来,用非放射性同位素地高辛标记作为探针,以经克隆测序知道IT15基因CAG重复次数为43次的个体作为阳性对照,正常个体作为阴性对照对20侧临床诊断为Huntington舞蹈病的疑似个体行Southern杂交检测.结果 11例病人排除Huntington舞蹈病,9名病人被确定为Huntington舞蹈病患者.结论 建立了用非放射性同位素地高辛标记的探针鉴定Huntington舞蹈病的Southern杂交方法,该方法准确可靠.

  1. Different domains of Bacillus thuringiensis delta-endotoxins can bind to insect midgut membrane proteins on ligand blots

    NARCIS (Netherlands)

    Maagd, de R.A.; Klei, van der H.; Bakker, P.L.; Stiekema, W.J.; Bosch, D.

    1996-01-01

    We investigated the role of the constituent domains of the CryIA(b) and CryIA(c) δ-endotoxins in binding to midgut epithelial cell membrane proteins of Spodoptera exigua and Manduca sexta on ligand blots. A collection of wild- type and CryIC-CryIA hybrid toxins was used for this purpose. As demonstr

  2. Comparison of Diagnostic Value of Antigen B and Protoscoleces Antigen in Diagnosis of Hydatid Cyst by Blotting Method

    Directory of Open Access Journals (Sweden)

    F. Oreizi

    2006-01-01

    Full Text Available Introduction & Objective : Hydatidosis, a disease caused by the cestod helminth echinococcus granulosus, is one of the most important parasitic zoonosis in man and a variety of animals. Sensitive and reliable serologic methods are necessary to confirm the diagnosis. In this study, Ag B and Psc Ag were purified as two specific parasitic antigens and evaluated by Dot blotting used on the serum of hydatidosis patients and control group in order to identify the most sensitive and specific subunits.Materials and Methods: In an analytic and comparative study, serum samples collected from 22 patients under operation of hydatid cyst. As a control group, 4 patients with acute toxoplasmosis, 4 patients with leishmaniasis, 4 patients infected by non-hydatid cestods(Tenia saginata and H.nana and 4 normal subjects were included in this investigation. Infected sheep’s liver and lung were used for the preparation of antigen. Cyst fluid containing protoscoleces was extracted and then partially purified with a protein A column. AgB and Psc Ags were interacted with hydatid and control sera, with Dot blot method and sensitivity and specificity of these antigens were evaluated. Results: Sensitivity and specificity were estimated 95.9% and 81% respectively, for AgB and 100% and 63% respectively, for Psc Ag in Dot blot Method. Conclusion: Evaluation of sensitivity and specificity of AgB and Psc Ag using Dot blotting revealed that AgB has high value for diagnosis of hydatidosis. and presumably can help physicians to diagnose hydatid cyst easier than other routine tests.

  3. Differentiation of Enterococcus faecium from Lactobacillus delbrueckii subsp. bulgaricus and Streptococcus thermophilus strains by PCR and dot-blot hybridisation.

    Science.gov (United States)

    Langa, S; Fernández, A; Martín, R; Reviriego, C; Marín, M L; Fernández, L; Rodríguez, J M

    2003-12-01

    Variations in length and sequence of the 16S/23S spacer region of Enterococcus faecium provided the basis for development of simple PCR and dot-blot hybridisation assays that enabled the differentiation of potentially probiotic Enterococcus faecium strains from Lactobacillus delbrueckii subsp. bulgaricus and Streptococcus thermophilus. Such assays may be useful for differentiation of yoghurt starter cultures and enterococcal strains when they are simultaneously present in probiotic food products.

  4. Development of PCR/dot blot assay for specific detection and differentiation of taeniid cestode eggs in canids.

    Science.gov (United States)

    Armua-Fernandez, Maria Teresa; Nonaka, Nariaki; Sakurai, Tatsuya; Nakamura, Seita; Gottstein, Bruno; Deplazes, Peter; Phiri, Isaac G K; Katakura, Ken; Oku, Yuzaburo

    2011-01-01

    We report the development of a colourimetric PCR/dot blot assay targeting the mitochondrial gene NADH dehydrogenase subunit 1 (nad1) for differential diagnosis of taeniid eggs. Partial sequences of the cestode nad1 gene were aligned and new primers were designed based on conserved regions. Species-specific oligonucleotide probes (S-SONP) for canine taeniid cestodes were then designed manually based on the variable region between the conserved primers. Specifically, S-SONP were designed for the Taenia crassiceps, T. hydatigena, T. multiceps, T. ovis, T. taeniaeformis, Echinococcus granulosus (genotype 1), E. multilocularis and E. vogeli. Each probe showed high specificity as no cross-hybridisation with any amplified nad1 fragment was observed. We evaluated the assay using 49 taeniid egg-positive samples collected from dogs in Zambia. DNA from 5 to 10 eggs was extracted in each sample. Using the PCR/dot blot assay, the probes successfully detected PCR products from T. hydatigena in 42 samples, T. multiceps in 3 samples, and both species (mixed infection) in the remaining 4 samples. The results indicate that the PCR/dot blot assay is a reliable alternative for differential diagnosis of taeniid eggs in faecal samples.

  5. Investigation of Anti-Toxocara Antibodies in Epileptic Patients and Comparison of Two Methods: ELISA and Western Blotting

    Directory of Open Access Journals (Sweden)

    Mohammad Zibaei

    2013-01-01

    Full Text Available The relationship between Toxocara infection and epilepsy was previously demonstrated by several case-control studies and case reports. These previous studies were often based on the enzyme-linked immunosorbent assay (ELISA using Toxocara excretory-secretory antigens, which are not specific due to cross-reactivity with other parasitic infections such as ascariasis, trichuriasis, and anisakiasis. An immunoblot analysis is highly specific and can detect low levels of Toxocara antibodies. Therefore, this assay may be useful in the identification of toxocariasis in epileptic patients. We examined patients who had epilepsy and healthy subjects for seropositivity for Toxocara infection by ELISA and Western blotting. Out of 85 epileptic patients, 10 (11.8% and 3 (3.5% persons exhibited Toxocara immunoglobulin G (IgG antibodies responses by ELISA and by both techniques, respectively. Moreover, in the healthy group (, 3 (3.5% persons were positive by ELISA, but none was detected by Western blotting. This study indicates that Toxocara infection is a risk factor for epilepsy in Iran. These findings strongly suggest the need to perform Western blotting immunodiagnosis, as well as the ELISA using Toxocara excretory-secretory antigens, to improve diagnosis of human toxocariasis in patients with epilepsy.

  6. DETECTION OF BREAST CANCER MICROMETASTASES IN BONE MARROW USING REVERSE-TRANSCRIPTASE CHAIN REACTION AND SOUTHERN HYBRIDIZATION

    Institute of Scientific and Technical Information of China (English)

    LI Jin-feng; ZHANG Lei; SUN Su-lian

    1999-01-01

    Objective: The aim of this study was to detect micrometastases in bone marrow of primary breast cancer patients, and compare with other clinical parameters.Methods: Cytokeratin 19 (CK-19) gene mRNA expression was detected by reverse transcriptase-polymerase chain reaction (RT-PCR) and Southern blot hybridization.Human breast cancer cell line T47D was mixed with bone marrow cells in different proportions. The positive detection rate was compared among RT-PCR, Southern blotting and immunohistochemistry (IHC) methods. Results: Cytokeratin 19 gene was expressed in all 6 positive control samples while the expression was not seen in 8 negative control samples. In all 54 patients 14 cases were CK-19 positive (25.9%) by RT-PCR, another positive signal was obtained in 5/54 (9.3%) of bone marrow samples by Southern blotting. The total positive cases are 19/54 (35.2%).CK-19 IHC+ cells were detected at a dilution of one T47D cell in 5×104 bone marrow cells, while the sensitivity detected by PCR and Southern blot hybridization was at 1:5×105 and 1:1×106, respectively. This demonstrates that RT-PCR and Southern blotting was at least 20 times more sensitive than the IHC method. The micrometastases positive rate of the larger tumor size group (>5.0 cm) was significantly (P<0.05) greater than that of the smaller tumor size group (0-2.0 cm). Conclusion: detection of micrometastases in bone marrow by RT-PCR and Southern blotting, using CK-19 as a biological marker, is highly sensitive and it is a method to be used for anticipating the prognosis of breast cancer patients.

  7. Southern California Particle Center

    Data.gov (United States)

    Federal Laboratory Consortium — At the Southern California Particle Center, center researchers will investigate the underlying mechanisms that produce the health effects associated with exposure to...

  8. Detection of Rickettsia in Rhipicephalus sanguineus ticks and Ctenocephalides felis fleas from southeastern Tunisia by reverse line blot assay.

    Science.gov (United States)

    Khrouf, Fatma; M'Ghirbi, Youmna; Znazen, Abir; Ben Jemaa, Mounir; Hammami, Adnene; Bouattour, Ali

    2014-01-01

    Ticks (n = 663) and fleas (n = 470) collected from domestic animals from southeastern Tunisia were screened for Rickettsia infection using reverse line blot assay. Evidence of spotted fever group Rickettsia was obtained. We detected Rickettsia felis in fleas, Rickettsia massiliae Bar 29 and the Rickettsia conorii Israeli spotted fever strain in ticks, and Rickettsia conorii subsp. conorii and Rickettsia spp. in both arthropods. The sensitivity of the adopted technique allowed the identification of a new association between fleas and R. conorii subsp. conorii species. The presence of these vector-borne Rickettsia infections should be considered when diagnosing this disease in humans in Tunisia.

  9. How to Distinguish Between the Activity of HDAC1-3 and HDAC6 with Western Blot.

    Science.gov (United States)

    Beyer, Mandy; Kiweler, Nicole; Mahboobi, Siavosh; Krämer, Oliver H

    2017-01-01

    Histone deacetylases (HDACs) catalyze the deacetylation of lysine residues in their target proteins. This biochemical modification can have profound effects on the functions of these proteins and a dysregulation of HDAC activity contributes to severe diseases, including neoplastic transformation. In the following chapter, we present a strategy that allows to distinguish between the inhibition of the class I HDACs HDAC1, 2, and 3 and of the class IIb HDAC HDAC6. This method is based on Western blot and relies on the detection of hyperacetylated substrates of class I or class IIb HDACs in lysates from cells that were treated with histone deacetylase inhibitors (HDACi).

  10. Southern Identity in "Southern Living" Magazine

    Science.gov (United States)

    Lauder, Tracy

    2012-01-01

    A fantasy-theme analysis of the editors' letters in "Southern Living" magazine shows an editorial vision of valuing the past and showcasing unique regional qualities. In addition, a content analysis of the visual representation of race in the magazine's formative years and recent past validates that inhabitants of the region were portrayed…

  11. Analysis of Sperm Membrane Protein Relevant to Antisperm Antibody by Two-Dimensional Gel Electrophoresis and Western Blotting

    Institute of Scientific and Technical Information of China (English)

    Hao-fei WANG; Zhu-qiong XIANG; Yi-xing WANG

    2003-01-01

    Objective To identify the sperm membrane proteins that are associated with antisperm antibodyMethods Using antisperm antibody positive serum through unidimensional polyacrylamide gel electrophoresis and 2-dimensional gel electrophoresis followed by Western blot analysis to determine the molecular weights (MW) and isoelectric points (pI) of sperm membrane proteins that are associated with antisperm antibody.Results Eight kinds of MW with more than ten sperm membrane proteins can be recognized by antisperm antibody positive serum, of which the MWs and pI were 23 kD, 31 kD, 32 kD, 34 kD, 41 kD, 51 kD, 60 kD, 78 kD and 5.3, 5.5,5.7, 5.0, 5.3, 5.8, 6.0, 5.5~6.2, 4.6,5.1,5.5~5.8 respectively. The identification ratios of the sperm membrane proteins on 78 kD (60.7%), 60 kD (71.4%), 51 kD (14.9%) and 23 kD (14.29%) were higher.Conclusion The sperm membrane proteins with MW of 78 kD, 60 kD, 51 kD and 23 kD were associated with antisperm antibody and immunological infertility. Two-dimensional gel electrophoresis and Western blotting can precisely identify the sperm membrane proteins that are associated with antisperm antibody.

  12. Dot-Blot Hybridization for Detection of Five Cucurbit Viruses by Digoxigenin-Labelled cDNA Probes

    Institute of Scientific and Technical Information of China (English)

    MENG Juan; GU Qin-sheng; LIN Shi-ming; PENG Bin; LIU Li-feng; TIAN Yan-ping; LI Li

    2007-01-01

    Dot-blot hybridization was applied in this paper to detect five viruses infecting cucurbitaceous crops,Zuccini yellow mosaic virus(ZYMV),Watermelon mosaic virus(WMV),Cucumber mosaic virus(CMV),Papaya ringspot virus watermelon strain(PRSV-W)and Squash mosaic virus(SqMV),as a good alternative assay in seed health test and epidemiological and transgenic research.Digoxigenin-labelled cDNA probes of the five viruses were synthesized by PCR with the specific primers and applied in dot-blot hybridization to detect five viruses in crude extraction of the infected leaves.And three SqMV probes of different lengths(0.55,1.6,and 2.7 kb,respectively)were designed to investigate the effect of hybridization.The results showed that the sensitivity for detecting the crude extraction of infected leaves by ZYMV,WMV,CMV,PRSV-W,and SqMV was down to 1:160,1:160,1:320,1:160,and 1:320,respectively.Three SqMV probes of different length showed no differences on the sensitivity and specificity.The digoxigenin-labelled probes prepared by PCR could be used for accurate and rapid identification of 5 viruses infecting cucurbitaceous crops with good stabilities,sensitivities,specificity,and reproducibilities.

  13. Mere end blot pirringer

    DEFF Research Database (Denmark)

    Jantzen, Christian; Østergaard, Per

    2006-01-01

    Med udgangspunkt i en række cases på vellykkede oplevelsesøkonomiske forretningsmodeller argumenterer artiklen for, at oplevelsesprodukter skal bygge på et klart tema, som forbrugeren kan koble sig sanse- og følelsesmæssigt op på. Forbrugeren skal kunne omsætte produktets pirringer til egne erfar...

  14. Modification of T-cell antigenic properties of tetanus toxoid by SDS-PAGE separation. Implications for T-cell blotting

    DEFF Research Database (Denmark)

    Christensen, C B; Theander, T G

    1997-01-01

    Using Tetanus Toxoid (TT) as a model antigen the T-cell Blotting method was evaluated. Peripheral blood mononuclear cell (PBMC) cultures were stimulated by blotted nitrocellulose-bound TT or soluble TT. SDS-Poly-Acrylamide-Gel-Electrophoresis separated TT only induced proliferation in 20% of the ......Using Tetanus Toxoid (TT) as a model antigen the T-cell Blotting method was evaluated. Peripheral blood mononuclear cell (PBMC) cultures were stimulated by blotted nitrocellulose-bound TT or soluble TT. SDS-Poly-Acrylamide-Gel-Electrophoresis separated TT only induced proliferation in 20...... that SDS-PAGE alters the ability of TT to induce T-cell proliferation, possibly due to unpolymerized acrylamide binding to proteins during SDS-PAGE. The use of SDS-PAGE T-cell blotting in the screening for T-cell antigens must therefore be reconsidered. We suggest the use of SDS-Agarose Gel Electrophoresis...

  15. The application of a photon-counting camera in very sensitive, bioluminescence-enhanced detection systems for protein blotting. Ultrasensitive detection systems for protein blotting and DNA hybridization, II.

    Science.gov (United States)

    Hauber, R; Miska, W; Schleinkofer, L; Geiger, R

    1988-03-01

    A relatively simple, very sensitive bioluminescence-enhanced detection system for protein blots was described recently. This method utilizes antibodies conjugated with alkaline phosphatase. Alkaline phosphatase releases D-luciferin (Photinus pyralis) from D-luciferin-O-phosphate. Liberated D-luciferin reacts with luciferase, ATP and oxygen with light emission. The light produced is measured with a very sensitive photon counting camera (Argus 100), permitting visualization and localization of the alkaline phosphatase-conjugated antibodies on nitrocellulose sheets. Under non-optimized conditions the limit of detection is at present 5 to 500 fg of protein (rabbit immunoglobulin G), corresponding to 30 to 3 amol. The method is therefore 10(5) times more sensitive than other used at present.

  16. Epidemiological Investigation of Canine Leishmaniasis in Southern Morocco

    Directory of Open Access Journals (Sweden)

    Samia Boussaa

    2014-01-01

    Full Text Available Dogs are the major reservoir of Leishmania infantum, the causative agent of human and canine visceral leishmaniasis in the Mediterranean basin. In Morocco, canine leishmaniasis (CanL is usually believed to be widespread mainly, if not only, in the northern regions and few data are available about the situation in southern parts of the country. Here, we report the results of a preliminary, clinical, and serological study carried out in 2004–2007, in four provinces of southern Morocco. Serological analyses were processed using two different Elisa techniques, a homemade Elisa test and IDVET commercial kit, and confirmed by two different western blot (WB tests, homemade and LDBIO commercial kits. We highlighted the presence of CanL infection in southern regions, known until then as free of the disease: 19.8% (48/243 of examined dogs displayed clinical signs compatible with CanL and the seroprevalence was particularly high, respectively, 81.8% and 87.8% by Elisa and western blot tests. Our current developed and validated homemade (Elisa and WB tools will be cost-effective and useful for next large-scale epidemiological studies on Moroccan leishmaniasis animal reservoir.

  17. 用DNA探针检测沙眼衣原体%Detection of Chlamydia Trachomatis by DNA Probe Blot

    Institute of Scientific and Technical Information of China (English)

    王卫萍; 陈亚利; 武建国

    2001-01-01

    目的: 建立一种敏感而特异的沙眼衣原体分子生物学检测方法. 方法: 用PCR扩增517bp的沙眼衣原体种特异性基因片段并标记成探针,建立DNA探针杂交检测沙眼衣原体的方法.结果: 探针只与沙眼衣原体L2、TE55株DNA呈阳性杂交斑点,与其他两种衣原体、解脲支原体、淋病奈瑟菌、大肠埃希菌、金黄色葡萄球菌、流感嗜血杆菌及白色念珠菌DNA斑点膜无阳性杂交信号.从100例慢性宫颈炎和前列腺炎病人生殖道分泌物中检出阳性22例,阳性率22%. 结论: 建立的DNA探针检测沙眼衣原体方法具有较高的敏感性和特异性,可用于批量临床标本的检测.%Objectives: To establish a sensitive and specific molecular biologicalmethod for detecting Chlamydia trachomatis of genital tract infection patients. Methods: A DNA blot assay was developed by coating DNA of Chlamydia trachomatisand/or extract of clinical samples on nitrocellulose (NC) membrane, blotting witha DNA probe labeled with DIG. Results: There was no positive blotting in Chlamydiapneumoniae, Chlamydia psittaci, Ureaplasma urealyticum, Neisseria gonorrhoeae,Esherichia coli, Staphylococcus aureus, Haemophilus infuenzae and Candida albicansexcept Chlamydia trachomatis. The sensitivity could be improved to 1pg. The positivepercentage was 22% (22/100) in detection of swabs collected from 100 chroniccervicitis and prostatitis patients. Conclusions: This method was not only sensitive, rapid and specific but also could be applied to detect batch samples. Natl J Androl,2001,7(2):102~104

  18. Lectin staining and Western blot data showing differential sialylation of nutrient-deprived cancer cells to sialic acid supplementation.

    Science.gov (United States)

    Badr, Haitham A; AlSadek, Dina M M; Mathew, Mohit P; Li, Chen-Zhong; Djansugurova, Leyla B; Yarema, Kevin J; Ahmed, Hafiz

    2015-12-01

    This report provides data that are specifically related to the differential sialylation of nutrient deprived breast cancer cells to sialic acid supplementation in support of the research article entitled, "Nutrient-deprived cancer cells preferentially use sialic acid to maintain cell surface glycosylation" [1]. Particularly, breast cancer cells, when supplemented with sialic acid under nutrient deprivation, display sialylated glycans at the cell surface, but non-malignant mammary cells show sialylated glycans intracellularly. The impact of sialic acid supplementation under nutrient deprivation was demonstrated by measuring levels of expression and sialylation of two markers, EGFR1 and MUC1. This Data in Brief article complements the main manuscript by providing detailed instructions and representative results for cell-level imaging and Western blot analyses of changes in sialylation during nutrient deprivation and sialic acid supplementation. These methods can be readily generalized for the study of many types of glycosylation and various glycoprotein markers through the appropriate selection of fluorescently-labeled lectins.

  19. Standardisation of Western blotting to detect HTLV-1 antibodies synthesised in the central nervous system of HAM/TSP patients

    Directory of Open Access Journals (Sweden)

    Luiz Claudio Pereira Ribeiro

    2013-09-01

    Full Text Available Intrathecal synthesis of human T-lymphotropic virus type 1 (HTLV-1 antibodies (Abs represents conclusive evidence of a specific immune response in the central nervous system of HTLV-1 associated myelopathy/tropical spastic paraparesis (HAM/TSP patients. Western blotting (WB for HTLV Abs in serum is a confirmatory test for HTLV-1 infection. The aim of this study was to standardise the Western blot to demonstrate the intrathecal pattern of Abs against HTLV-1 proteins in HAM/TSP patients. Paired cerebrospinal fluid (CSF and serum samples were selected from 20 patients with definite HAM/TSP, 19 HTLV-1 seronegative patients and two HTLV-1 patients without definite HAM/TSP. The presence of reactive bands of greater intensity in the CSF compared to serum (or bands in only the CSF indicated the intrathecal synthesis of anti-HTLV-1 Abs. All definite HAM/TSP patients presented with an intrathecal synthesis of anti-HTLV-1 Abs; these Abs were not detected in the control patients. The most frequent intrathecal targets of anti-HTLV-1 Abs were GD21, rgp46-I and p24 and, to a lesser extent, p19, p26, p28, p32, p36, p53 gp21 and gp46. The intrathecal immune response against env (GD21 and rgp46-I and gag (p24 proteins represents the most important humoral pattern in HAM/TSP. This response may be used as a diagnostic marker, considering the frequent association of intrathecal anti-HTLV-1 Ab synthesis with HAM/TSP and the pathogenesis of this neurological disease.

  20. MDR-TB Antibody Response (Western Blot) to Fractions of Isoniazid and Rifampicin Resistant Antigens of Mycobacterium tuberculosis.

    Science.gov (United States)

    Hadizadeh Tasbiti, Alireza; Yari, Shamsi; Ghanei, Mostafa; Shokrgozar, Mohammad Ali; Bahrmand, Ahmadreza

    2015-12-01

    Drug-resistant TB poses a major threat to control of TB worldwide. Despite progress in the detection of Multidrug-resistant TB (MDR-TB) cases, a major diagnostic gap remains: 55% of reported TB patients estimated to have MDR-TB were not detected in 2013. MDR-TB antigens were conjugated to CNBr-activated Sepharose 4B. Specific polyclonal antibodies against MDR-TB Ags were prepared in rabbits using two boosted injections of the MDR-TB antigen. The antibodies were purified and treated with susceptible TB to remove any non-specific and cross-reactive antibodies. In the present study, comparative analysis of electrophoretic pattern of different antigens of INH/RIF-resistant TB were studied for identifying protein profiles. A RIF-resistant TB antigen was shown here to have different protein profiles from INH-resistant TB isolate. The results of Western blotting analysis showed that in the RIF- and INH-resistant antigenic fractions some bands of 14.4 and 45 kDa as immunogenic were common. Moreover, four bands of RIF-resistant TB antigen fractions (16, 19, 21, and 45 KDa) and one band of INH-resistant TB (about 26 KDa) were detected as diagnostic antigens. This study suggests that the Western blot is an accurate test to survey INH- and RIF-resistant TB antigens of M. tuberculosis infection. These findings indicate that MDR-TB diagnosis (based on Ag detection) could be useful in the identification of disease stages that precede symptomatic and microbiologically positive TB, such as subclinical and incipient TB.

  1. University of Southern California

    Data.gov (United States)

    Federal Laboratory Consortium — The focus of the University of Southern California (USC) Children''s Environmental Health Center is to develop a better understanding of how host susceptibility and...

  2. European Southern Observatory

    CERN Multimedia

    1970-01-01

    Professor A. Blaauw, Director general of the European Southern Observatory, with George Hampton on his right, signs the Agreement covering collaboration with CERN in the construction of the large telescope to be installed at the ESO Observatory in Chile.

  3. Earthquakes in Southern California

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — There have been many earthquake occurrences in Southern California. This set of slides shows earthquake damage from the following events: Imperial Valley, 1979,...

  4. Southern hemisphere observations

    Science.gov (United States)

    Orchiston, Wayne

    Because of insurmountable problems associated with absolute dating, the non-literate cultures of the Southern Hemisphere can contribute little to Applied Historical Astronomy, although Maori traditions document a possible supernova dating to the period 1000-1770 AD. In contrast, the abundant nineteenth century solar, planetary, cometary and stellar observational data provided by Southern Hemisphere professional and amateur observatories can serve as an invaluable mine of information for present-day astronomers seeking to incorporate historical data in their investigations.

  5. [Optimization of the immunoelectrophoresis technic (western blot) for the confirmation of human immunodeficiency virus infection (HIV) in Panama].

    Science.gov (United States)

    Pascale, J M; de Austin, E; de Moreno, N O; Ledezma, C; de Márquez, E; Blanco, R; Quiroz, E; Calzada, J; Vincensini, A R; de Martin, M C

    1995-01-01

    The purpose of this study is to report the results of the authors' investigation to apply the western blot technique (WB UP-LCS) in the diagnosis of human immunodeficiency virus type 1 (HIV-1) infection. To do this, the authors separated the proteins of the HIV-1 virus by electrophoresis, based on their molecular weight, in poliacilamide gel with SDS (SDS-PAGE) during 3 hours at 200 volts. Then they electrotransferred these proteins to nitrocellulose paper during four hours at 200 milliamperes, with the aid of external cooling. The nitrocellulose strips were evaluated considering the incubation time (1 and 16 hours), two conjugates (human anti IgG with Peroxidase and human anti IgG Biotin plus Streptatividine with Peroxidase) and two dilutions of the patients' sera (1/50 and 1/100). Based on their results the Authors conclude that, in the first place, the optimal conditions for the test include a dilution of 1/100 of the patients serum, incubation of the serum for 16 hours and the use of the conjugate of anti human IgG with Biotin and Streptavidine with Peroxidase; secondary, that the immunologic reactivity against proteins p24 and gp 160/120 is the most important diagnostic criterion for the confirmation of infection with HIV-1 and that they obtained a diagnostic correlation of 100% at a cost which was 5 to 7 times less than that of the commercial system.

  6. Development of rapid, sensitive and non-radioactive tissue-blot diagnostic method for the detection of citrus greening.

    Science.gov (United States)

    Nageswara-Rao, Madhugiri; Miyata, Shin-Ichi; Ghosh, Dilip; Irey, Mike; Garnsey, Stephen M; Gowda, Siddarame

    2013-01-01

    Citrus huanglongbing (HLB or citrus greening) is one of the most devastating diseases of citrus worldwide. The disease is caused by Gram-negative, phloem-limited α-proteobacterium, 'Candidatus Liberibacter asiaticus', vectored by the psyllid, Diaphorina citri Kuwayama. Citrus plants infected by the HLB bacterium may not show visible symptoms sometimes for years following infection and non-uniform distribution within the tree makes the detection of the pathogen very difficult. Efficient management of HLB disease requires rapid and sensitive detection early in the infection followed by eradication of the source of pathogen and the vector. The polymerase chain reaction (PCR) based method is most commonly employed for screening the infected/suspected HLB plants and psyllids. This is time consuming, cumbersome and not practical for screening large number of samples in the field. To overcome this, we developed a simple, sensitive, non-radioactive, tissue-blot diagnostic method for early detection and screening of HLB disease. Digoxigenin labeled molecular probes specific to 'Ca. L. asiaticus' nucleotide sequences have been developed and used for the detection of the pathogen of the HLB disease. The copy number of the target genes was also assessed using real-time PCR experiments and the optimized real-time PCR protocol allowed positive 'Ca. L. asiaticus' detection in citrus samples infected with 'Ca. L. asiaticus' bacterium.

  7. Cross-Reactions between Toxocara canis and Ascaris suum in the diagnosis of visceral larva migrans by western blotting technique

    Directory of Open Access Journals (Sweden)

    NUNES Cáris Maroni

    1997-01-01

    Full Text Available Visceral larva migrans (VLM is a clinical syndrome caused by infection of man by Toxocara spp, the common roundworm of dogs and cats. Tissue migration of larval stages causes illness specially in children. Because larvae are difficult to detect in tissues, diagnosis is mostly based on serology. After the introduction of the enzyme-linked immunosorbent assay (ELISA using the larval excretory-secretory antigen of T. canis (TES, the diagnosis specificity was greatly improved although cross-reactivity with other helminths are still being reported. In Brazil, diagnosis is routinely made after absorption of serum samples with Ascaris suum antigens, a nematode antigenicaly related with Ascaris lumbricoides which is a common intestinal nematode of children. In order to identify T. canis antigens that cross react to A. suum antigens we analyzed TES antigen by SDS-PAGE and Western blotting techniques. When we used serum samples from patients suspected of VLM and positive result by ELISA as well as a reference serum sample numerous bands were seen (molecular weight of 210-200 kDa, 116-97 kDa, 55-50 kDa and 35-29 kDa. Among these there is at least one band with molecular weight around 55-66 kDa that seem to be responsible for the cross-reactivity between T. canis e A. suum once it disappears when previous absorption of serum samples with A. suum antigens is performed

  8. Western blotting using Strongyloides ratti antigen for the detection of IgG antibodies as confirmatory test in human strongyloidiasis

    Directory of Open Access Journals (Sweden)

    Luciana Pereira Silva

    2003-07-01

    Full Text Available The present study was conducted to evaluate the frequency of antigenic components recognized by serum IgG antibodies in Western blotting (WB using a Strongyloides ratti larval extract for the diagnosis of human strongyloidiasis. In addition, the WB results were compared to the enzyme-linked immunosorbent assay (ELISA and the indirect immunofluorescence antibody test (IFAT results. Serum samples of 180 individuals were analyzed (80 with strongyloidiasis, 60 with other intestinal parasitoses, and 40 healthy individuals. S. ratti was obtained from fecal culture of experimentally infected Rattus rattus. For IFAT, S. ratti larvae were used as antigen and S. ratti larval antigenic extracts were employed in WB and ELISA. Eleven S. ratti antigenic components were predominantly recognized by IgG antibodies in sera of patients with strongyloidiasis. There was a positive concordance for the three tests in 87.5% of the cases of strongyloidiasis. The negative concordance in the three tests was 94% and 97.5%, in patients with other intestinal parasitoses and healthy individuals, respectively. In cases of positive ELISA and negative IFAT results, diagnosis could be confirmed by WB. ELISA, IFAT, and WB using S. ratti antigens showed a high rate of sensitivity and specificity. In conclusion, WB using S. ratti larval extract was able to recognize 11 immunodominant antigenic components, showing to be a useful tool to define the diagnosis in cases of equivocal serology.

  9. "Enzyme-Linked Immunotransfer Blot Analysis of Somatic and Excretory- Secretory Antigens of Fasciola hepatica in Diagnosis of Human Fasciolosis"

    Directory of Open Access Journals (Sweden)

    MB Rokni

    2004-07-01

    Full Text Available The liver fluke Fasciola hepatica causes fascioliasis, a liver disease in most part of the world and particularly in north of Iran. Diagnosis of the diseases is anchored in coprological manner but serological methods are preferable due to some obscurities. In this study, sera obtained from human patients infected with Fasciola hepatica were tested by the enzymelinked immunotrotransfer blot (EITB technique with the parasite s somatic and excretory-secretory (ES antigens in order to evaluate the diagnostic potential of the assay. The study included sera from 40 patients infected with F. hepatica, 20 infected with hydatidosis, 6 with toxocariasis, 10 with strongyloidiasis, 10 with amoebiasis, 5 with malaria and 30 normal controls. By this assay, most pf the serum samples from humans with fascioliasis recognized two antigenic polypeptides of 27 and 29 kDa using both antigens. The sensitivity, specificity, positive and negative predictive values for somatic antigen were 91.0%, 96.2%, 95.2% and 92.7% respectively, while these parameters as for ES antigen were 95.2%, 98.0%, 97.5% and 96.2%, correspondingly. Totally, two cases of reactions for the first antigen and one for the latter were verified. The study suggests that the 27 and 29 kDa bands for two antigens in EITB test could be considered for the immunodiagnosis of human fascioliasis.

  10. Lectin staining and Western blot data showing differential sialylation of nutrient-deprived cancer cells to sialic acid supplementation

    Directory of Open Access Journals (Sweden)

    Haitham A. Badr

    2015-12-01

    Full Text Available This report provides data that are specifically related to the differential sialylation of nutrient deprived breast cancer cells to sialic acid supplementation in support of the research article entitled, “Nutrient-deprived cancer cells preferentially use sialic acid to maintain cell surface glycosylation" [1]. Particularly, breast cancer cells, when supplemented with sialic acid under nutrient deprivation, display sialylated glycans at the cell surface, but non-malignant mammary cells show sialylated glycans intracellularly. The impact of sialic acid supplementation under nutrient deprivation was demonstrated by measuring levels of expression and sialylation of two markers, EGFR1 and MUC1. This Data in Brief article complements the main manuscript by providing detailed instructions and representative results for cell-level imaging and Western blot analyses of changes in sialylation during nutrient deprivation and sialic acid supplementation. These methods can be readily generalized for the study of many types of glycosylation and various glycoprotein markers through the appropriate selection of fluorescently-labeled lectins.

  11. Evaluation of western blotting for the diagnosis of enzootic bovine leukemia Avaliação da técnica de western blot no diagnóstico da leucose enzoótica bovina

    Directory of Open Access Journals (Sweden)

    E.T. Gonzalez

    1999-08-01

    Full Text Available A western blotting (WB procedure has been developed for detecting antibodies to bovine leukosis virus (BLV in cattle sera. Two hundred and thirty three serum samples from naturally infected cattle with BLV virus and serial bleedings from experimentally BLV infected cows were used. An agar gel immunodiffusion test (AGID was used for comparing with the results obtained by WB. The AGID positive sera showed a different degree of reactivity by WB test against the two most important viral antigens (gp51 and p24, or against one of them. Other proteins (gp30, p15, p12 and p10 were not detected with any AGID positive sera, being observed occasionally three bands corresponding to the p24 protein. Using sera obtained by BLV experimental inoculation, the antibodies directed to p24 appeared early (between the 2nd and 4th week post inoculation and thereafter antibodies to gp51were detected in some animals. The analysis of field serum samples by AGID as compared to WB showed an agreement of 90.9%. Only 1.7% of sera were negative by AGID and positive by WB and 7.2% that were not conclusive by AGID and were defined by WB (4.2% as positive and 3.0% as negative.Um sistema de western blotting (WB foi desenvolvido para detecção de anticorpos contra o vírus da leucose em soros de bovinos. Foram utilizadas amostras de soros de 233 animais naturalmente infectados e soros de vacas experimentalmente infectadas. O teste de imunodifusão em ágar (AGID foi usado para comparação dos resultados. Graus diferentes de reatividade foram observados em soros positivos ao AGID, quando testados em WB frente a um ou aos dois antígenos mais importantes (gp51 e p24. Outras proteínas (gp30, p15, p12 e p10 não foram detectadas por nenhum soro positivo ao AGID, sendo que três bandas correspondentes à proteína p24 foram observadas ocasionalmente. Em soros obtidos por inoculação experimental, anticorpos contra a proteína p24 foram detectados entre a segunda e a quarta semanas

  12. Evaluation of ELISA coupled with Western blot as a surveillance tool for Trichinella infection in wild boar (Sus scrofa).

    Science.gov (United States)

    Cuttell, Leigh; Gómez-Morales, Maria Angeles; Cookson, Beth; Adams, Peter J; Reid, Simon A; Vanderlinde, Paul B; Jackson, Louise A; Gray, C; Traub, Rebecca J

    2014-01-31

    Trichinella surveillance in wildlife relies on muscle digestion of large samples which are logistically difficult to store and transport in remote and tropical regions as well as labour-intensive to process. Serological methods such as enzyme-linked immunosorbent assays (ELISAs) offer rapid, cost-effective alternatives for surveillance but should be paired with additional tests because of the high false-positive rates encountered in wildlife. We investigated the utility of ELISAs coupled with Western blot (WB) in providing evidence of Trichinella exposure or infection in wild boar. Serum samples were collected from 673 wild boar from a high- and low-risk region for Trichinella introduction within mainland Australia, which is considered Trichinella-free. Sera were examined using both an 'in-house' and a commercially available indirect-ELISA that used excretory-secretory (E/S) antigens. Cut-off values for positive results were determined using sera from the low-risk population. All wild boar from the high-risk region (352) and 139/321 (43.3%) of the wild boar from the low-risk region were tested by artificial digestion. Testing by Western blot using E/S antigens, and a Trichinella-specific real-time PCR was also carried out on all ELISA-positive samples. The two ELISAs correctly classified all positive controls as well as one naturally infected wild boar from Gabba Island in the Torres Strait. In both the high- and low-risk populations, the ELISA results showed substantial agreement (k-value=0.66) that increased to very good (k-value=0.82) when WB-positive only samples were compared. The results of testing sera collected from the Australian mainland showed the Trichinella seroprevalence was 3.5% (95% C.I. 0.0-8.0) and 2.3% (95% C.I. 0.0-5.6) using the in-house and commercial ELISA coupled with WB respectively. These estimates were significantly higher (PTrichinella DNA in any mainland animals, but did reveal the presence of a second larvae-positive wild boar on Gabba

  13. Estrogen receptors alpha and beta in rat placenta: detection by RT-PCR, real time PCR and Western blotting

    Directory of Open Access Journals (Sweden)

    Al-Bader Maie D

    2006-03-01

    Full Text Available Abstract Background High levels of estrogens during pregnancy not only retard placental and fetal growth but can lead to reproductive tract abnormalities in male progeny. Estrogens act through estrogen receptors (ER to modulate the transcription of target genes. These ER exist in two isoforms, ER alpha and ER beta and recently several variants of these isoforms have been identified. Methods The expressions of ER isoforms and variants have been studied in rat placenta at 16, 19 and 21 days gestation (dg. Gene expression was assessed using RT-PCR and real time PCR while protein expression was studied using Western blotting followed by immunodetection. Placental homogenates were probed with: a monoclonal antibody raised against the steroid binding domain of the ER alpha (ER alpha -S, a monoclonal antibody raised against the hinge region of ER alpha (ER alpha -H and a polyclonal antibody raised against the amino terminus of ER beta. Results ER alpha and ER beta mRNA and protein were detected from as early as 16 dg. Two PCR products were detected for ER alpha, one for the wild type ER alpha, and a smaller variant. Real time PCR results suggested the presence of a single product for ER beta. The antibodies used for detection of ER alpha protein both identified a single 67 kDa isoform; however a second 54 kDa band, which may be an ER alpha variant, was identified when using the ER alpha -H antibody. The abundance of both ER alpha bands decreased significantly between 16 and 19 dg. As for ER beta, four bands (76, 59, 54 and 41 kDa were detected. The abundance of the 59 and 54 kDa bands decreased significantly between 16 and 19 dg. Conclusion This study shows that both ER protein isoforms and their variants are present in rat placenta. The decrease in their expression near parturition suggests that the placenta may be relatively unresponsive to estrogens at this stage.

  14. Multilingualism in Southern Africa.

    Science.gov (United States)

    Peirce, Bonny Norton; Ridge, Stanley G. M.

    1997-01-01

    Reviews recent research in multilingualism in Southern Africa, focusing on the role of languages in education, sociolinguistics, and language policy. Much of the research is on South Africa. Topics discussed include language of instruction in schools, teacher education, higher education, adult literacy, language contact, gender and linguistic…

  15. Molecular diagnosis of Theileria and Babesia species infecting cattle in Northern Spain using reverse line blot macroarrays

    Directory of Open Access Journals (Sweden)

    Juste Ramón A

    2006-05-01

    Full Text Available Abstract Background Piroplasmosis in cattle is caused by tick-borne haemoprotozoan parasites of the genera Theileria and Babesia. Molecular detection techniques offer higher sensitivity and specificity than microscopy examination methods and serological tests. A reverse line blot (RLB macroarray that included generic and species-specific probes for Theileria annulata, Theileria buffeli, Babesia bovis, Babesia bigemina, Babesia divergens and Babesia major was used to study the presence and identity of the piroplasm species infecting 263 bovine blood samples from 79 farms, most of them in Northern Spain. Microscopy examination of blood smears and haematology were also performed whenever possible to identify animals with parasitaemia. Results RLB hybridisation identified infection in 54.0% of the samples, whereas only 28.8% were positive by microscopy examination. The most frequently found species was T. buffeli, present in 42.6% of the samples. T. annulata was found in 22 samples (8.4% from 12 farms, including 9 farms (14 samples located in Northern Spain where presence of the vector is not very common. Babesia infections were less frequently detected: B. major was found in 3.0% of the samples, B. bigemina in 2.7%, B. bovis in 2.3% and B. divergens in 1.1%. Mixed infections were detected in 14 samples, accounting for six different combinations of species. Conclusion This is the first report in which B. major and B. divergens have been detected in Spain using molecular identification techniques and the first time that B. bovis has been detected in Northern Spain. The detection of T. annulata in Northern Spain suggests that the distribution of Mediterranean theileriosis might be changing. Samples with positive RLB hybridisation but negative microscopy had haematology values within the normal ranges suggesting that they corresponded to chronic carriers that may serve as reservoirs of the infection. In this sense, sensitive and specific laboratorial

  16. Evaluation of two sets of immunohistochemical and Western blot confirmatory methods in the detection of typical and atypical BSE cases

    Directory of Open Access Journals (Sweden)

    Greenlee Justin J

    2011-09-01

    Full Text Available Abstract Background Three distinct forms of bovine spongiform encephalopathy (BSE, defined as classical (C-, low (L- or high (H- type, have been detected through ongoing active and passive surveillance systems for the disease. The aim of the present study was to compare the ability of two sets of immunohistochemical (IHC and Western blot (WB BSE confirmatory protocols to detect C- and atypical (L- and H-type BSE forms. Obex samples from cases of United States and Italian C-type BSE, a U.S. H-type and an Italian L-type BSE case were tested in parallel using the two IHC sets and WB methods. Results The two IHC techniques proved equivalent in identifying and differentiating between C-type, L-type and H-type BSE. The IHC protocols appeared consistent in the identification of PrPSc distribution and deposition patterns in relation to the BSE type examined. Both IHC methods evidenced three distinct PrPSc phenotypes for each type of BSE: prevailing granular and linear tracts pattern in the C-type; intraglial and intraneuronal deposits in the H-type; plaques in the L-type. Also, the two techniques gave comparable results for PrPSc staining intensity on the C- and L-type BSE samples, whereas a higher amount of intraglial and intraneuronal PrPSc deposition on the H-type BSE case was revealed by the method based on a stronger demasking step. Both WB methods were consistent in identifying classical and atypical BSE forms and in differentiating the specific PrPSc molecular weight and glycoform ratios of each form. Conclusions The study showed that the IHC and WB BSE confirmatory methods were equally able to recognize C-, L- and H-type BSE forms and to discriminate between their different immunohistochemical and molecular phenotypes. Of note is that for the first time one of the two sets of BSE confirmatory protocols proved effective in identifying the L-type BSE form. This finding helps to validate the suitability of the BSE confirmatory tests for BSE

  17. Relative performance of Organon kit in comparison to Du Pont for confirmatory serological testing of HIV infection by western blot test in sera from blood donors.

    Science.gov (United States)

    Aggarwal, R K; Chatterjee, R; Chattopadhya, D; Kumari, S

    1992-06-01

    A total of 32 specimens with different categories of reactivity by Du Pont Western Blot kit comprising of specimens showing full spectrum of HIV-I antigen specific bands, 19 specimens showing total absence of bands and four specimens showing non-specific bands (without any interpretative importance) were subjected to Western Blot testing by Organon test. Of the nine specimens showing full spectrum of bands by Du Pont the correlation with Organon kit was 100 per cent based on WHO criteria. Four specimens with non-specific indeterminate band pattern by Du Pont failed to show any band in Organon kit, indicating that latter to be more specific.

  18. Astrophysics in Southern Africa

    CERN Document Server

    Whitelock, Patricia A

    2007-01-01

    The government of South Africa has identified astronomy as a field in which their country has a strategic advantage and is consequently investing very significantly in astronomical infrastructure. South Africa now operates a 10-m class optical telescope, the Southern African Large Telescope (SALT), and is one of two countries short listed to host the Square Kilometre Array (SKA), an ambitious international project to construct a radio telescope with a sensitivity one hundred times that of any existing telescope. The challenge now is to produce an indigenous community of users for these facilities, particularly from among the black population which was severely disadvantaged under the apartheid regime. In this paper I briefly describe the observing facilities in Southern Africa before going on to discuss the various collaborations that are allowing us to use astronomy as a tool for development, and at the same time to train a new generation of astronomers who will be well grounded in the science and linked to ...

  19. Two Interesting Southern Objects

    Science.gov (United States)

    Gyulbudaghian, A. L.

    2016-06-01

    Two southern objects are studied. The first, the planetary nebula PK 349-01.1, is of interest because it has a chain of jets ejected from the central star. 12C(1-0) observations of the vicinity of this object reveal red- and blue-shifted molecular outflows. The second object is a star formation region consisting of two groups of IR stars. These groups have a trapezium-like configuration. Two stars in one of these groups are associated with a ring-shaped nebulae. This star formation region is associated with a new radial system of dark globules.

  20. Astrophysics in Southern Africa

    Science.gov (United States)

    Whitelock, Patricia

    2008-03-01

    The government of South Africa has identified astronomy as a field in which their country has a strategic advantage and is consequently investing very significantly in astronomical infrastructure. South Africa now operates a 10-m class optical telescope, the Southern African Large Telescope (SALT), and is one of two countries short listed to host the Square Kilometre Array (SKA), an ambitious international project to construct a radio telescope with a sensitivity one hundred times that of any existing telescope. The challenge now is to produce an indigenous community of users for these facilities, particularly from among the black population which was severely disadvantaged under the apartheid regime. In this paper I briefly describe the observing facilities in Southern Africa before going on to discuss the various collaborations that are allowing us to use astronomy as a tool for development, and at the same time to train a new generation of astronomers who will be well grounded in the science and linked to their colleagues internationally.

  1. Combined Detection of Breast Cancer Micrometastases in the Lymph Nodes and Bone Marrow Using Reverse-transcriptase chain Reaction and Southern Hybridization

    Institute of Scientific and Technical Information of China (English)

    2000-01-01

    The presence of lymph nodes and bone marrow micrometastases of patients with breast carcinoma by immunohistochemistry (IHC) methods has been strongly correlated to early recurrence and shorter overall survival. The aim of this study was to detect micrometastases in matched sample pairs of lymph nodes and the bone marrow of primary breast cancer patients using a more sensitive method, and compare with other clinical parameters. Methods: Cytokeratin 19 (CK-19) gene mRNA expression was detected by reverse transcriptase-polymerase chain reaction (RT-PCR) and Southern blot hybridization. Human breast cancer cell line T47D was mixed with bone marrow cells at different proportions. The positive detection rate was compared among RT-PCR, Southern blotting and IHC methods. Results: Cytokeratin 19 gene was expressed in all 6 positive control samples, while the expression wasn't seen in 18 negative control samples. CK-19 IHC positive cells were detected at a dilution of one T47D cell in 5×105 bone marrow cells, while the sensitivity detected by PCR and Southern blot hybridization was at 1:5′ 104 and 1:106, respectively. In the samples from the 35 patients, we found CK-19 positive cells in 2 cases (5.7%) by IHC. CK-19 gene expression signal was detected in 14/35 (40%) by RT-PCR,and 17/35 (48.6%) by southern blotting. Four cases were micrometastases positive both in lymph node and bone marrow (11.4%). There was no correlation between CK-1 9 detection and other clinical parameters. Conclusion: combined detection of micrometastases in lymph node and bone marrow by RT-PCR and Southern blotting, using CK-19 as a biological marker, is a highly sensitive method for breast cancer.

  2. Characterization of Sm14 related components in different helminths by sodium dodecyl sulphate-polyacrylamide gel electrophoresis and Western blotting analysis

    Directory of Open Access Journals (Sweden)

    Nilton Thaumaturgo

    2002-10-01

    Full Text Available Sm14 was the first fatty acid-binding protein homologue identified in helminths. Thereafter, members of the same family were identified in several helminth species, with high aminoacid sequence homology between them. In addition, immune crossprotection was also reported against Fasciola hepatica infection, in animals previously immunized with the Schistosoma mansoni vaccine candidate, r-Sm14. In the present study, data on preliminary sodium dodecyl sulphate-polyacrylamide gel electrophoresis and Western blotting analysis of nine different helminth extracts focusing the identification of Sm14 related proteins, is reported. Out of these, three extracts - Ascaris suum (males and females, Echinostoma paraensei, and Taenia saginata - presented components that comigrated with Sm14 in SDS-PAGE, and that were recognized by anti-rSm14 policlonal serum, in Western blotting tests.

  3. Analysis of the Crude Antigen of Hymenolepis nana from Mice by SDS-PAGE and the Determination of Specific Antigens in Protein Structure by Western Blotting

    OpenAIRE

    GÖNENÇ, Bahadır

    2002-01-01

    Protein bands of crude antigens of Hymenolepis nana were determined by SDS-PAGE and Western blotting. Thirty Swiss albino mice were allotted into two groups of 15 each as positive (infected with H. nana) and negative (non-infected with H. nana) groups. The natural infections of H. nana and other helminths were determined by centrifugal flotation of faeces. After bleeding, the mice were necropsied and their guts were examined for H. nana and other intestinal helminths. Sera from mice were test...

  4. Western blot analysis of a limited number of cells: a valuable adjunct to proteome analysis of paraffin wax-embedded, alcohol-fixed tissue after laser capture microdissection.

    Science.gov (United States)

    Martinet, Wim; Abbeloos, Vanessa; Van Acker, Nathalie; De Meyer, Guido R Y; Herman, Arnold G; Kockx, Mark M

    2004-03-01

    In recent years, laser capture microdissection (LCM) has been used successfully to obtain distinct populations of cells for subsequent molecular analysis. Because of the limited sample availability and the absence of in vitro amplification steps for proteins, the use of LCM for proteome analysis largely depends on highly sensitive protein detection methods. In this study, a western blot protocol was developed and validated for the detection of beta-actin and the moderately expressed cell death protein caspase-3 in small numbers of cells. Initially, cultured human U937 monocytes and whole sections of paraffin wax-embedded, alcohol-fixed human tonsils were used to optimize protein electrophoresis and western blotting conditions. High-performance NuPAGE Bis-Tris gels in combination with high-quality transfer membranes, optimized antibody concentrations, and a sensitive chemiluminescent substrate provided a strong signal for beta-actin with approximately 500 U937 cells. In the same way, procaspase-3 could be identified with approximately 1000 cells. Similar results were obtained with germinal centre cells that were procured from paraffin wax-embedded, alcohol-fixed human tonsils by LCM. Treatment of U937 cells with etoposide rapidly induced cell death and allowed the detection of active caspase-3 with approximately 2500 cells (0.8 pg of protein). The findings of this study suggest that western blotting is a valuable adjunct to proteome analysis of LCM procured cells.

  5. Quantitative Expression Analysis in Brassica napus by Northern Blot Analysis and Reverse Transcription-Quantitative PCR in a Complex Experimental Setting.

    Science.gov (United States)

    Rumlow, Annekathrin; Keunen, Els; Klein, Jan; Pallmann, Philip; Riemenschneider, Anja; Cuypers, Ann; Papenbrock, Jutta

    Analysis of gene expression is one of the major ways to better understand plant reactions to changes in environmental conditions. The comparison of many different factors influencing plant growth challenges the gene expression analysis for specific gene-targeted experiments, especially with regard to the choice of suitable reference genes. The aim of this study is to compare expression results obtained by Northern blot, semi-quantitative PCR and RT-qPCR, and to identify a reliable set of reference genes for oilseed rape (Brassica napus L.) suitable for comparing gene expression under complex experimental conditions. We investigated the influence of several factors such as sulfur deficiency, different time points during the day, varying light conditions, and their interaction on gene expression in oilseed rape plants. The expression of selected reference genes was indeed influenced under these conditions in different ways. Therefore, a recently developed algorithm, called GrayNorm, was applied to validate a set of reference genes for normalizing results obtained by Northern blot analysis. After careful comparison of the three methods mentioned above, Northern blot analysis seems to be a reliable and cost-effective alternative for gene expression analysis under a complex growth regime. For using this method in a quantitative way a number of references was validated revealing that for our experiment a set of three references provides an appropriate normalization. Semi-quantitative PCR was prone to many handling errors and difficult to control while RT-qPCR was very sensitive to expression fluctuations of the reference genes.

  6. Fires in Southern California

    Science.gov (United States)

    2007-01-01

    In what seemed like the blink of an eye, wildfires ignited in the paper-dry, drought-stricken vegetation of Southern California over the weekend of October 20, 2007, and exploded into massive infernos that forced hundreds of thousands of people to evacuate their communities. Driven by Santa Ana winds, fires grew thousands of acres in just one to two days. The fires sped down from the mountains into the outskirts of coastal cities, including San Diego. Dozens of homes have burned to the ground, and at least one person has died, according to local news reports. Several of the fires were burning completely out of control as of October 22. This image of the fires in California was captured at 1:55 p.m. U.S. Pacific Daylight Time on October 22, 2007. Places where MODIS detected actively burning fires are outlined in red. Thick streamers of smoke unfurl over the Pacific Ocean. The brownish plumes are clouds of dust. Fires northwest of Los Angeles seemed calmer at the time of this image than they were the previous day.

  7. Glycophospholipid Formulation with NADH and CoQ10 Significantly Reduces Intractable Fatigue in Western Blot-Positive ‘Chronic Lyme Disease’ Patients: Preliminary Report

    Directory of Open Access Journals (Sweden)

    Garth L. Nicolson

    2012-03-01

    Full Text Available Background: An open label 8-week preliminary study was conducted in a small number of patients to determine if a combination oral supplement containing a mixture of phosphoglycolipids, coenzyme Q10 and microencapsulated NADH and other nutrients could affect fatigue levels in long-term, Western blot-positive, multi-symptom ‘chronic Lyme disease’ patients (also called ‘post-treatment Lyme disease’ or ‘post Lyme syndrome’ with intractable fatigue. Methods: The subjects in this study were 6 males (mean age = 45.1 ± 12.4 years and 10 females (mean age = 54.6 ± 7.4 years with ‘chronic Lyme disease’ (determined by multiple symptoms and positive Western blot analysis that had been symptomatic with chronic fatigue for an average of 12.7 ± 6.6 years. They had been seen by multiple physicians (13.3 ± 7.6 and had used many other remedies, supplements and drugs (14.4 ± 7.4 without fatigue relief. Fatigue was monitored at 0, 7, 30 and 60 days using a validated instrument, the Piper Fatigue Scale.Results: Patients in this preliminary study responded to the combination test supplement, showing a 26% reduction in overall fatigue by the end of the 8-week trial (p< 0.0003. Analysis of subcategories of fatigue indicated that there were significant improvements in the ability to complete tasks and activities as well as significant improvements in mood and cognitive abilities. Regression analysis of the data indicated that reductions in fatigue were consistent and occurred with a high degree of confidence (R2= 0.998. Functional Foods in Health and Disease 2012, 2(3:35-47 Conclusions: The combination supplement was a safe and effective method to significantly reduce intractable fatigue in long-term patients with Western blot-positive ‘chronic Lyme disease.’

  8. Study of a viral-dual infection in rainbow trout (Oncorhynchus mykiss) by seroneutralization, western blot and polymerase chain reaction assays.

    Science.gov (United States)

    Rodríguez, S; Vilas, M P; Alonso, M; Pérez, S I

    1995-12-01

    Viral-dual infections in fish are of interest to aquaculture practices but they are rarely described and studied. Several methods were applied in this work to demonstrate a case of coinfection in a reared rainbow trout (Oncorhynchus mykiss) population. Inoculation in cell cultures and cross-neutralization tests were the standard procedures that made it possible to isolate and identify a birnavirus, the infectious pancreatic necrosis virus (IPNV), and suspect of a second virus. Western blotting with both polyclonal and monoclonal antibodies, and reverse transcriptional-polymerase chain reaction (RT-PCR) demonstrate coexistence of both, IPNV and a rhabdovirus.

  9. APPLICATION OF WESTERN BLOTTING TECHNIQUE FOR EVALUATING THE EXPRESSION OF VASOPRESSIN RECEPTORS IN THE HEART CELLS; IMPORTANCE IN THE CARDIOVASCULAR SYSTEM

    Directory of Open Access Journals (Sweden)

    Manoj G Tyagi

    2012-08-01

    Full Text Available Vasopressin, a posterior pituitary hormone is responsible for water reabsorption by the kidneys and maintenance of cardio-vascular homeostasis. Vasopressin receptors are characterized as VR 1 (V1a, VR2 (V2, and VR3 (V1b. VR1, which is abundant in vascular smooth muscles, causes vasoconstriction by increasing intracellular calcium via the phosphatidylinositol bisphosphonate pathway and a positive inotropic effect in cardiac muscle. VR2 has also been shown to be expressed in the heart. There is emerging role for vasopressin receptors in health and disease. This study describes the application of Western blotting to elucidate the importance of vasopressin receptors in the heart cells.

  10. Invertebrate diversity in southern California

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This shapefile displays mean invertebrate diversity within 5 minute grid cells. The Shannon Index of diversity was calculated from Southern California Coastal Water...

  11. Erythrineae (Fabaceae in southern Africa

    Directory of Open Access Journals (Sweden)

    E. F. Franklin Hennessy

    1991-12-01

    Full Text Available The two genera represented in the flora of southern Africa.  Erythrina L. and  Mucuna Adans. are revised. Keys to the indigenous species and the commonly cultivated exotic species are provided.

  12. Diagnosis of facioscapulohumeral muscular dystrophy using double enzyme digestion associated Southern blotting method%应用双酶切/Southern杂交方法诊断面肩肱型肌营养不良

    Institute of Scientific and Technical Information of China (English)

    王朝东; 吴志英; 王柠; 林珉婷; 方玲; 王志强; 慕容慎行

    2002-01-01

    目的探讨面肩肱型肌营养不良(FSHD)的基因诊断方法.方法抽取我院1997~2000年收治的37例FSHD患者的静脉血,常规抽提gDNA,以EcoRI/HindⅢ、EcoRI/BlnⅠ分别酶切gDNA,0.6%琼脂糖凝胶电泳分离,p13E-11探针Southern杂交,应用ImageMaster Total Lab v1.11分析软件判断杂交片段大小.结果正常对照只检测到1个4q35来源的、大于33 kb的EcoRI+HindⅢ/p13E-11片段,而所有FSHD患者中有33例检测到2个4q35来源的EcoRI+HindⅢ/p13E-11片段,其中包含1个小于33 kb的小片段;3例患者中可检测到2个小于33 kb的片段,但其中1个来自10q26;另1例散发性患者中检测到3个4q35-型片段,且其中2个小于33 kb.在1名无症状9岁男孩中检测到1个与其患病父亲一致的小片段,提示为症状前患者.结论双酶切/Southern杂交的方法可用于中国人面肩肱型肌营养不良患者的基因诊断及症状前诊断.本文结果首次提示我国FSHD患者中也存在4q-10q的相互易位现象.

  13. Alport syndrome in southern Sweden

    DEFF Research Database (Denmark)

    Persson, U; Hertz, Jens Michael; Wieslander, J

    2005-01-01

    The aim of the present investigation is to study the epidemiology of Alport syndrome in southern Sweden, to search for mutations in the COL4A5 gene and to estimate the mutation frequency.......The aim of the present investigation is to study the epidemiology of Alport syndrome in southern Sweden, to search for mutations in the COL4A5 gene and to estimate the mutation frequency....

  14. A Comparison of Antibacterial Activity of Selected Thyme (Thymus) Species by Means of the Dot Blot Test with Direct Bioautographic Detection.

    Science.gov (United States)

    Orłowska, Marta; Kowalska, Teresa; Sajewicz, Mieczysław; Jesionek, Wioleta; Choma, Irena M; Majer-Dziedzic, Barbara; Szymczak, Grażyna; Waksmundzka-Hajnos, Monika

    2015-01-01

    Bioautography carried out with the aid of thin-layer chromatographic adsorbents can be used to assess antibacterial activity in samples of different origin. It can either be used as a simple and cost-effective detection method applied to a developed chromatogram, or to the dot blot test performed on a chromatographic plate, where total antibacterial activity of a sample is scrutinized. It was an aim of this study to compare antibacterial activity of 18 thyme (Thymus) specimens and species (originating from the same gardening plot and harvested in the same period of time) by means of a dot blot test with direct bioautography. A two-step extraction of herbal material was applied, and at step two the polar fraction of secondary metabolites was obtained under the earlier optimized extraction conditions [methanol-water (27+73, v/v), 130°C]. This fraction was then tested for its antibacterial activity against Bacillus subtilis bacteria. It was established that all investigated extracts exhibited antibacterial activity, yet distinct differences were perceived in the size of the bacterial growth inhibition zones among the compared thyme species. Based on the results obtained, T. citriodorus "golden dwarf" (sample No. 5) and T. marschallianus (sample No. 6) were selected as promising targets for further investigations and possible inclusion in a herbal pharmacopeia, which is an essential scientific novelty of this study.

  15. Detection of genetically modified microorganisms in soil using the most-probable-number method with multiplex PCR and DNA dot blot.

    Science.gov (United States)

    Yeom, Jinki; Lee, Yunho; Noh, Jaemin; Jung, Jaejoon; Park, Jungsoon; Seo, Hyoju; Kim, Jisun; Han, Jiwon; Jeon, Che Ok; Kim, Taesung; Park, Woojun

    2011-10-01

    The principal objective of this study was to detect genetically modified microorganisms (GMMs) that might be accidentally released into the environment from laboratories. Two methods [plate counting and most-probable-number (MPN)] coupled with either multiplex PCR or DNA dot blots were compared using genetically modified Escherichia coli, Pseudomonas putida, and Acinetobacter oleivorans harboring an antibiotic-resistance gene with additional gfp and lacZ genes as markers. Alignments of sequences collected from databases using the Perl scripting language (Perl API) and from denaturing gradient gel electrophoresis analysis revealed that the gfp, lacZ and antibiotic-resistance genes (kanamycin, tetracycline, and ampicillin) in GMMs differed from the counterpart genes in many sequenced genomes and in soil DNA. Thus, specific multiplex PCR primer sets for detection of plasmid-based gfp and lacZ antibiotic-resistance genes could be generated. In the plate counting method, many antibiotic-resistant bacteria from a soil microcosm grew as colonies on antibiotic-containing agar plates. The multiplex PCR verification of randomly selected antibiotic-resistant colonies with specific primers proved ineffective. The MPN-multiplex PCR method and antibiotic-resistant phenotype could be successfully used to detect GMMs, although this method is quite laborious. The MPN-DNA dot blot method screened more cells at a time in a microtiter plate containing the corresponding antibiotics, and was shown to be a more efficient method for the detection of GMMs in soil using specific probes in terms of labor and accuracy.

  16. Southern Dust Devils

    Science.gov (United States)

    2004-01-01

    [figure removed for brevity, see original site] Released 9 July 2004 The atmosphere of Mars is a dynamic system. Water-ice clouds, fog, and hazes can make imaging the surface from space difficult. Dust storms can grow from local disturbances to global sizes, through which imaging is impossible. Seasonal temperature changes are the usual drivers in cloud and dust storm development and growth. Eons of atmospheric dust storm activity has left its mark on the surface of Mars. Dust carried aloft by the wind has settled out on every available surface; sand dunes have been created and moved by centuries of wind; and the effect of continual sand-blasting has modified many regions of Mars, creating yardangs and other unusual surface forms. In our final dust devil image we are again looking at the southern hemisphere of Mars. These tracks occur mainly on the northeast side of the topographic ridges. Of course, there are many exceptions, which makes understanding the dynamics that initiate the actual dust devil cyclone difficult. Image information: VIS instrument. Latitude -47.6, Longitude 317.3 East (42.7 West). 19 meter/pixel resolution. Note: this THEMIS visual image has not been radiometrically nor geometrically calibrated for this preliminary release. An empirical correction has been performed to remove instrumental effects. A linear shift has been applied in the cross-track and down-track direction to approximate spacecraft and planetary motion. Fully calibrated and geometrically projected images will be released through the Planetary Data System in accordance with Project policies at a later time. NASA's Jet Propulsion Laboratory manages the 2001 Mars Odyssey mission for NASA's Office of Space Science, Washington, D.C. The Thermal Emission Imaging System (THEMIS) was developed by Arizona State University, Tempe, in collaboration with Raytheon Santa Barbara Remote Sensing. The THEMIS investigation is led by Dr. Philip Christensen at Arizona State University. Lockheed

  17. Treasures of the Southern Sky

    CERN Document Server

    Gendler, Robert; Malin, David

    2011-01-01

    In these pages, the reader can follow the engaging saga of astronomical exploration in the southern hemisphere, in a modern merger of aesthetics, science, and a story of human endeavor. This book is truly a celebration of southern skies.  Jerry Bonnell, Editor - Astronomy Picture of the Day The southern sky became accessible to scientific scrutiny only a few centuries ago, after the first European explorers ventured south of the equator. Modern observing and imaging techniques have since revealed what seems like a new Universe, previously hidden below the horizon, a fresh astronomical bounty of beauty and knowledge uniquely different from the northern sky. The authors have crafted a book that brings this hidden Universe to all, regardless of location or latitude. Treasures of the Southern Sky celebrates the remarkable beauty and richness of the southern sky in words and with world-class imagery. In part, a photographic anthology of deep sky wonders south of the celestial equator, this book also celebrates th...

  18. GAPDH and β-actin protein decreases with aging, making Stain-Free technology a superior loading control in Western blotting of human skeletal muscle

    DEFF Research Database (Denmark)

    Vigelsø Hansen, Andreas; Dybboe, Rie; Hansen, Christina Neigaard;

    2015-01-01

    [β-actin, glyceraldehyde 3-phosphate dehydrogenase (GAPDH), and α-tubulin], as well as TP loaded measured by Stain-Free technology (SF) as normalization tool were tested. This was done using skeletal muscle samples from men subjected to physiological conditions often investigated in applied...... of β-actin and GAPDH was lower in older men compared with young men. In conclusion, β-actin, GAPDH, and α-tubulin may not be used for normalization in studies that include subjects with a large age difference. In contrast, the RPs may not be affected in studies that include muscle wasting...... and differences in muscle fiber type. The novel SF technology adds lower variation to the results compared with the existing methods for correcting for loading inaccuracy in Western blotting of human skeletal muscle in applied physiology....

  19. Serum detection of IgG antibodies against Demodex canis by western blot in healthy dogs and dogs with juvenile generalized demodicosis.

    Science.gov (United States)

    Ravera, Ivan; Ferreira, Diana; Gallego, Laia Solano; Bardagí, Mar; Ferrer, Lluís

    2015-08-01

    The aim of this study was to investigate the presence of canine immunoglobulins (Ig) G against Demodex proteins in the sera of healthy dogs and of dogs with juvenile generalized demodicosis (CanJGD) with or without secondary pyoderma. Demodex mites were collected from dogs with CanJGD. Protein concentration was measured and a western blot technique was performed. Pooled sera from healthy dogs reacted mainly with antigen bands ranging from 55 to 72 kDa. Pooled sera from dogs with CanJGD without secondary pyoderma reacted either with 10 kDa antigen band or 55 to 72 kDa bands. Pooled sera from dogs with CanJGD with secondary pyoderma reacted only with a 10 kDa antigen band. The results of this study suggest that both healthy dogs and dogs with CanJGD develop a humoral response against different proteins of Demodex canis.

  20. A comparison of antigenic peptides in muscle larvae of several Trichinella species by two-dimensional western-blot analysis with monoclonal antibodies

    Directory of Open Access Journals (Sweden)

    Dea-Ayuela M.A.

    2001-06-01

    Full Text Available The antigens recognised by mAb US5 specific to 53 kDa glycoprotein (gp 53 in T. spiralis L-1 muscle larvae (TSL1 antigens, mAb US9 specific to gp 53 in TSL1 from all encapsulated species and mAb US4 specific to a tyvelose containing tetrasaccharide present in TSL1, were investigated in crude extracts from muscle larvae of T. spiralis, T. nativa and T. britovi by 2D-electrophoresis and western-blot. At least four proteins of different pI were recognised by mAb US5 on T. spiralis antigens. Recognition profile of mAb US9 on T. spiralis antigens exhibited some variation with regard to that of the US5. Polymorphism was apparent in gp 53. High reactivity was shown by the mAb US4 with the three species.

  1. Isolation and RNA gel blot analysis of genes that could serve as potential molecular markers for leaf senescence in Arabidopsis thaliana.

    Science.gov (United States)

    Yoshida, S; Ito, M; Nishida, I; Watanabe, A

    2001-02-01

    Nine cDNAs, representing genes in which the transcripts accumulated in senescent leaves of Arabidopsis thaliana, were isolated by differential display reverse transcription polymerase chain reaction (DDRT-PCR) and the genes were designated yellow-leaf-specific gene 1 to 9 (YLS1-YLS9). Sequence analysis revealed that none of the YLS genes, except YLS6, had been reported as senescence-up-regulated genes. RNA gel blot analysis revealed that the transcripts of YLS3 accumulated at the highest level at an early senescence stage, whereas the transcripts from the other YLS genes reached their maximum levels in late senescence stages. Transcripts of YLS genes showed various accumulation patterns under natural senescence, and under artificial senescence induced by darkness, ethylene or ABA. These expression characteristics of YLS genes will be useful as potential molecular markers, which will enhance our understanding of natural and artificial senescence processes.

  2. Localization and Distribution of 'Candidatus Liberibacter asiaticus' in Citrus and Periwinkle by Direct Tissue Blot Immuno Assay with an Anti-OmpA Polyclonal Antibody.

    Science.gov (United States)

    Ding, Fang; Duan, Yongping; Paul, Cristina; Brlansky, Ronald H; Hartung, John S

    2015-01-01

    'Candidatus Liberibacter asiaticus' (CaLas), a non-cultured member of the α-proteobacteria, is the causal agent of citrus Huanglongbing (HLB). Due to the difficulties of in vitro culture, antibodies against CaLas have not been widely used in studies of this pathogen. We have used an anti-OmpA polyclonal antibody based direct tissue blot immunoassay to localize CaLas in different citrus tissues and in periwinkle leaves. In citrus petioles, CaLas was unevenly distributed in the phloem sieve tubes, and tended to colonize in phloem sieve tubes on the underside of petioles in preference to the upper side of petioles. Both the leaf abscission zone and the junction of the petiole and leaf midrib had fewer CaLas bacteria compared to the main portions of the petiole and the midribs. Colonies of CaLas in phloem sieve tubes were more frequently found in stems with symptomatic leaves than in stems with asymptomatic leaves with an uneven distribution pattern. In serial sections taken from the receptacle to the peduncle, more CaLas were observed in the peduncle sections adjacent to the stem. In seed, CaLas was located in the seed coat. Many fewer CaLas were found in the roots, as compared to the seeds and petioles when samples were collected from trees with obvious foliar symptoms. The direct tissue blot immuno assay was adapted to whole periwinkle leaves infected by CaLas. The pathogen was distributed throughout the lateral veins and the results were correlated with results of qPCR. Our data provide direct spatial and anatomical information for CaLas in planta. This simple and scalable method may facilitate the future research on the interaction of CaLas and host plant.

  3. Localization and Distribution of 'Candidatus Liberibacter asiaticus' in Citrus and Periwinkle by Direct Tissue Blot Immuno Assay with an Anti-OmpA Polyclonal Antibody.

    Directory of Open Access Journals (Sweden)

    Fang Ding

    Full Text Available 'Candidatus Liberibacter asiaticus' (CaLas, a non-cultured member of the α-proteobacteria, is the causal agent of citrus Huanglongbing (HLB. Due to the difficulties of in vitro culture, antibodies against CaLas have not been widely used in studies of this pathogen. We have used an anti-OmpA polyclonal antibody based direct tissue blot immunoassay to localize CaLas in different citrus tissues and in periwinkle leaves. In citrus petioles, CaLas was unevenly distributed in the phloem sieve tubes, and tended to colonize in phloem sieve tubes on the underside of petioles in preference to the upper side of petioles. Both the leaf abscission zone and the junction of the petiole and leaf midrib had fewer CaLas bacteria compared to the main portions of the petiole and the midribs. Colonies of CaLas in phloem sieve tubes were more frequently found in stems with symptomatic leaves than in stems with asymptomatic leaves with an uneven distribution pattern. In serial sections taken from the receptacle to the peduncle, more CaLas were observed in the peduncle sections adjacent to the stem. In seed, CaLas was located in the seed coat. Many fewer CaLas were found in the roots, as compared to the seeds and petioles when samples were collected from trees with obvious foliar symptoms. The direct tissue blot immuno assay was adapted to whole periwinkle leaves infected by CaLas. The pathogen was distributed throughout the lateral veins and the results were correlated with results of qPCR. Our data provide direct spatial and anatomical information for CaLas in planta. This simple and scalable method may facilitate the future research on the interaction of CaLas and host plant.

  4. Southern Ocean Iron Experiment (SOFex)

    Energy Technology Data Exchange (ETDEWEB)

    Coale, Kenneth H.

    2005-07-28

    The Southern Ocean Iron Experiment (SOFeX) was an experiment decades in the planning. It's implementation was among the most complex ship operations that SIO has been involved in. The SOFeX field expedition was successful in creating and tracking two experimentally enriched areas of the Southern Ocean, one characterized by low silicic acid, one characterized by high silicic acid. Both experimental sites were replete with abundant nitrate. About 100 scientists were involved overall. The major findings of this study were significant in several ways: (1) The productivity of the southern ocean is limited by iron availability. (2) Carbon uptake and flux is therefore controlled by iron availability (3) In spite of low silicic acid, iron promotes non-silicious phytoplankton growth and the uptake of carbon dioxide. (4) The transport of fixed carbon from the surface layers proceeds with a C:N ratio that would indicate differential remineralization of nitrogen at shallow depths. (5) These finding have major implications for modeling of carbon export based on nitrate utilization. (6) The general results of the experiment indicate that, beyond other southern ocean enrichment experiments, iron inputs have a much wider impact of productivity and carbon cycling than previously demonstrated. Scientific presentations: Coale, K., Johnson, K, Buesseler, K., 2002. The SOFeX Group. Eos. Trans. AGU 83(47) OS11A-0199. Coale, K., Johnson, K. Buesseler, K., 2002. SOFeX: Southern Ocean Iron Experiments. Overview and Experimental Design. Eos. Trans. AGU 83 (47) OS22D-01. Buesseler, K.,et al. 2002. Does Iron Fertilization Enhance Carbon Sequestration? Particle flux results from the Southern Ocean Iron Experiment. Eos. Trans. AGU 83 (47), OS22D-09. Johnson, K. et al. 2002. Open Ocean Iron Fertilization Experiments From IronEx-I through SOFeX: What We Know and What We Still Need to Understand. Eos. Trans. AGU 83 (47), OS22D-12. Coale, K. H., 2003. Carbon and Nutrient Cycling During the

  5. Range Extent for southern sea otters 2016

    Data.gov (United States)

    U.S. Geological Survey, Department of the Interior — The GIS shapefile "Range extent of southern sea otters 2016" is a simple polyline representing the geographic distribution of the southern sea otter (Enhydra lutris...

  6. Storage and stability of IgG and IgM monoclonal antibodies dried on filter paper and utility in Neisseria meningitidis serotyping by Dot-blot ELISA

    Directory of Open Access Journals (Sweden)

    Yto André Y

    2008-03-01

    Full Text Available Abstract Background A simple filter paper method was developed for, the transport and storage of monoclonal antibodies (Mabs at room temperature or -20°C after spotting on filter paper, for subsequent serotyping of outer membrane antigens of N.meningitidis by dot-blot ELISA. Methods Monoclonal antibodies (Mabs were spotted within a 0.5–1 cm diameter area of Whatman grade 903 paper, which were stored individually at room temperature or at -20°C. These MAbs were stored and analyzed after periods of one week, 4 weeks, 12 months, or 13 years in the case of frozen Mab aliquots, or after 4 weeks at -20°C or at room temperature (RT in the case of Mabs dried on filter paper strips. Assays were performed in parallel using dot-blot ELISA. In addition to the MAbs specific for serotyping class 1, 2 or 3, we used a larger number of Mabs for polysaccharides, lipooligosaccharides (LOS, class 5 and cross-reactive antigens for native outer membrane of N.meningitidis. The Mabs dried on filter paper were eluted with phosphate-buffered saline (PBS containing 0.2% gelatin. Results Mabs of the isotypes IgG and IgM dried on filter papers were not affected by duration of storage. The detection by serotyping Mabs was generally consistent for dried filter paper MAb samples stored frozen for over 1 year at -20°C, and although decreased reactive antibody titers were found after storage, this did not interfere with the specificity of the Mabs used after 13 years as dry spots on filter paper. Conclusion The use of filter paper is an inexpensive and convenient method for collecting, storing, and transporting Mab samples for serotyping studies. In addition, the samples occupy little space and can be readily transported without freezing. The efficiency of using immunoglobulin G (IgG or M (IgM eluted was found to be consistent with measurement of IgG or IgM titers in most corresponding, ascites Mabs stored frozen for over 1 year. The application of meningococcal typing

  7. Utility of slot-blot-ELISA as a new, fast, and sensitive immunoassay for detection of carcinoembryonic antigen in the urine samples of patients with various gastrointestinal malignancies.

    Science.gov (United States)

    El-Masry, Samir; El-Sayed, Ibrahim H; Lotfy, Mahmoud; Mahmoud, Lamiaa; El-Naggar, Mohamed

    2007-01-01

    Carcinoembryonic antigen (CEA) is the most widely used clinical tumor marker. CEA immunoassay has found acceptance as a diagnostic adjunct in clinical diagnosis of gastrointestinal tumors (GIT). Several immunoassays have been established for detection of CEA in plasma, serum, tissue, feces, and urine of cancer patients using polyclonal or monoclonal antibodies raised against CEA. Some of these assays display both high sensitivity and specificity for the detection of CEA. However, these assays require special and highly expensive equipment and the procedures require long periods for their completion. In the present study, we established a Slot-Blot Enzyme Linked Immunosorbent Assay (SB-ELISA), based on anti-CEA monoclonal antibody (CEA-mAb), as a new, simple, fast, cheap, and non-invasive immunodiagnostic technique for detection of CEA in the urine of GIT patients. Urine and serum samples were collected from 248 GIT patients (58 with pancreatic cancer, 20 with hepatoma, 23 with ampullary carcinoma, 15 with hilar cholangiocarcinoma, 28 with gastric cancer, 14 with esophageal cancer, and 90 with colorectal cancer). Moreover, urine and serum samples were collected from 50 healthy individuals to serve as negative controls. The traditional ELISA technique was used for determination of CEA in the sera of GIT patients using anti-CEA monoclonal antibody. A comparison between the results of both techniques (ELISA and SB-ELISA) was carried out. The traditional ELISA detected CEA in the sera of 154 out of 248 GIT patients with a sensitivity of 59.8%, 51.7% positive predictive value (PPV) and 75.37% negative predictive value (NPV). In addition, it identified 15 false positive cases out of 50 healthy individuals with a specificity of 70%. The urinary CEA was identified by a Western blotting technique and CEA-mAb at a molecular mass of 180 Kda. The developed SB-ELISA showed higher sensitivity, specificity, PPV, and NPV (70.1%, 78%, 62.4%, and 82.13%, respectively) for detection

  8. A comparison of DNA extraction protocols from blood spotted on FTA cards for the detection of tick-borne pathogens by Reverse Line Blot hybridization.

    Science.gov (United States)

    Hailemariam, Zerihun; Ahmed, Jabbar Sabir; Clausen, Peter-Henning; Nijhof, Ard Menzo

    2017-01-01

    An essential step in the molecular detection of tick-borne pathogens (TBPs) in blood is the extraction of DNA. When cooled storage of blood under field conditions prior to DNA extraction in a dedicated laboratory is not possible, the storage of blood on filter paper forms a promising alternative. We evaluated six DNA extraction methods from blood spotted on FTA Classic(®) cards (FTA cards), to determine the optimal protocol for the subsequent molecular detection of TBPs by PCR and the Reverse Line Blot hybridization assay (RLB). Ten-fold serial dilutions of bovine blood infected with Babesia bovis, Theileria mutans, Anaplasma marginale or Ehrlichia ruminantium were made by dilution with uninfected blood and spotted on FTA cards. Subsequently, DNA was extracted from FTA cards using six different DNA extraction protocols. DNA was also isolated from whole blood dilutions using a commercial kit. PCR/RLB results showed that washing of 3mm discs punched from FTA cards with FTA purification reagent followed by DNA extraction using Chelex(®) resin was the most sensitive procedure. The detection limit could be improved when more discs were used as starting material for the DNA extraction, whereby the use of sixteen 3mm discs proved to be most practical. The presented best practice method for the extraction of DNA from blood spotted on FTA cards will facilitate epidemiological studies on TBPs. It may be particularly useful for field studies where a cold chain is absent.

  9. Whole genomic sequencing of RT98 mitochondria derived from Oryza rufipogon and northern blot analysis to uncover a cytoplasmic male sterility-associated gene.

    Science.gov (United States)

    Igarashi, Keisuke; Kazama, Tomohiko; Motomura, Keiji; Toriyama, Kinya

    2013-02-01

    Cytoplasmic male sterility (CMS) is a maternally inherited trait resulting in the failure to produce functional pollen and is often observed when an alien cytoplasm is transferred into a cultivated species. An RT98A CMS line and an RT98C fertility restorer line were obtained by successive backcrossing between Oryza rufipogon W1109 and Oryza sativa cultivar Taichung 65. To uncover the CMS-associated mitochondrial genes, we determined the complete sequence of the RT98-CMS mitochondrial genome using next-generation pyrosequencing, and searched new open reading frames (orfs) absent in a reported mitochondrial genome of O. sativa Nipponbare. Then, six candidates were selected for the CMS-associated genes based on the criteria in which they were chimeric in structure or encoded a peptide with transmembrane domains. One of the candidates, orf113, showed different transcript sizes between RT98A and RT98C on Northern blot analysis. The orf113 gene was shown to be co-transcribed with atp4 and cox3 encoding ATP synthase F0 subunit 4 and Cyt c oxidase subunit 3, respectively, and their transcripts were distinctly processed in the presence of a fertility restorer gene. Our results indicate that orf113 is a CMS-associated gene of RT98-CMS.

  10. Western blot seroindeterminate individuals for Human T-lymphotropic Virus 1/2 (HTLV-1/2 in Fortaleza (Brazil: a serological and molecular diagnostic and epidemiological approach

    Directory of Open Access Journals (Sweden)

    Terezinha de Jesus Teixeira Santos

    2003-06-01

    Full Text Available How to handle Western blot (WB seroindeterminate individuals for Human T-lymphotropic Virus 1/2 (HTLV-1/2 constitutes a challenge for blood banks and fam ilies. We made a cross-sectional study of 191 enzyme linked immunoassay (EIA reactive individuals from the hematological center (HEMOCE of Fortaleza (Brazil, examining their serological (WB and molecular (PCR diagnosis, and demographic profiles, as well as a possible association of their condition with other infectious pathologies and risk factors. Ethical institutional approval and personal consent were obtained. Out of 191 EIA reactive individuals, 118 were WB seroindeterminate and 73 were seropositive for HTLV-1/2. In the PCR analysis of 41 WB seroindeterminate individuals, 9 (22% were positive and 32 (78% were negative for HTLV-1/2. The demographic analysis indicated a trend towards a predominance of males among the seroindeterminate individuals and females in the seropositive ones. The seroindeterminate individuals were younger than the seropositive ones. We did not find any association of these conditions with syphilis, Chagas disease or HIV or hepatitis, and with risk factors such as breast-feeding, blood transfusion, STD (syphilis and IDU.

  11. Utility of Schistosoma bovis Adult Worm Antigens for Diagnosis of Human Schistosomiasis by Enzyme-Linked Immunosorbent Assay and Electroimmunotransfer Blot Techniques

    Science.gov (United States)

    Pardo, J.; Carranza, C.; Turrientes, M. C.; Arellano, J. L. Pérez; Vélez, R. López; Ramajo, V.; Muro, A.

    2004-01-01

    Immunodiagnostic methods based on the detection of antibodies continue to be the most effective and practical methods for the diagnosis of imported schistosomiasis. Schistosoma bovis is a species whose final natural hosts are bovines, ovines, caprines, and small wild ruminants. Different studies have demonstrated the analogies existing between S. bovis and other Schistosoma species which affect humans. The objective of this work was to evaluate the utility of S. bovis adult worm antigens (AWA) for the diagnosis of imported human schistosomiasis by enzyme-linked immunosorbent assay (ELISA) and electroimmunotransfer blotting (EITB) techniques. By detecting eggs, the ELISA for S. bovis AWA was able to definitively detect imported cases with a sensitivity of 94%. The specificity of the ELISA for S. bovis AWA was 97%. There were no differences between the results of the S. bovis AWA ELISA for patients infected with Schistosoma mansoni and those infected with Schistosoma haematobium. The EITB technique showed bands of 85, 37, and 20 kDa, which are characteristic of infections with Schistosoma spp. Specific bands to indicate infection by different species of Schistosoma have not been detected. The combined use of the ELISA for S. bovis AWA and EITB increased the global sensitivity of the study to 97%. Our findings suggest that the ELISA for S. bovis AWA is a useful test for the immunodiagnosis of imported schistosomiasis and that EITB for detecting S. bovis AWA permits the confirmation of diagnosis when the ELISA for S. bovis AWA is positive. PMID:15539523

  12. Diversity of Borrelia genospecies in Ixodes ricinus ticks in a Lyme borreliosis endemic area in Switzerland identified by using new probes for reverse line blotting.

    Science.gov (United States)

    Gern, Lise; Douet, Véronique; López, Zully; Rais, Olivier; Cadenas, Francisca Morán

    2010-03-01

    In Europe, 7 Borrelia species belonging to the Borrelia burgdorferi sensu lato complex have been reported in Ixodes ricinus ticks. In addition, another Borrelia, related to the relapsing fever spirochaetes, has also been described. In the present study, we designed probes for reverse line blotting allowing detection and identification of all these Borrelia species after amplification of the variable spacer region between the 23S and 5S ribosomal genes. These new probes allowed us investigate the diversity of Borrelia in 915 I. ricinus collected on the south-facing slope of Chaumont (Switzerland). Among the 159 infected ticks, 7 Borrelia species were identified, and B. spielmanii and relapsing fever-like (RFL) spirochaetes were identified in this area for the first time. B. valaisiana and B. spielmanii were significantly less present in male than in female or nymphal ticks. Mixed infection with RFL spirochaetes and Lyme borreliosis spirochaetes were detected in 4 ticks. In addition, the set of probes could identify the recently described species, B. bavariensis.

  13. A Western blot-based investigation of the yeast secretory pathway designed for an intermediate-level undergraduate cell biology laboratory.

    Science.gov (United States)

    Hood-Degrenier, Jennifer K

    2008-01-01

    The movement of newly synthesized proteins through the endomembrane system of eukaryotic cells, often referred to generally as the secretory pathway, is a topic covered in most intermediate-level undergraduate cell biology courses. An article previously published in this journal described a laboratory exercise in which yeast mutants defective in two distinct steps of protein secretion were differentiated using a genetic reporter designed specifically to identify defects in the first step of the pathway, the insertion of proteins into the endoplasmic reticulum (Vallen, 2002). We have developed two versions of a Western blotting assay that serves as a second way of distinguishing the two secretory mutants, which we pair with the genetic assay in a 3-wk laboratory module. A quiz administered before and after students participated in the lab activities revealed significant postlab gains in their understanding of the secretory pathway and experimental techniques used to study it. A second survey administered at the end of the lab module assessed student perceptions of the efficacy of the lab activities; the results of this survey indicated that the experiments were successful in meeting a set of educational goals defined by the instructor.

  14. Application of the Reverse Line Blot Assay for the Molecular Detection of Theileria and Babesia sp. in Sheep and Goat Blood Samples from Pakistan

    Directory of Open Access Journals (Sweden)

    A Rasul

    2013-06-01

    Full Text Available Background: The present study was designed to detect the presence of tick-borne parasites (Theileria and Babesia spp. in 196 blood samples collected from apparently healthy sheep and goats from two provinces, Punjab and Khyber Pukhtoon Khwa, in Pakistan.Methods: Reverse line blot (RLB assay was applied for the parasitic detection by the amplification of hypervariable V4 region of the 18S ribosomal RNA (rRNA gene. A membrane with covalently linked generic and species specific oligonucleotide probes was used for the hybridization of amplified PCR products.Results: Parasites were detected in 16% of the ruminant blood samples under study. Two Theileria species, T. lestoquardi and T. ovis, were identified in samples. 25, of the total 32, infected animals were from Khyber Pukhtoon Khwa.Conclusion: Sheep were more prone to tick borne haemoprotozans as 81% infected samples were sheep as compared to 19% goats (P > 0.001. Risk factor analysis revealed that male (P = 0.03, ani­mals infested by ticks (P = 0.03 and herd composed of sheep only (P = 0.001 were more infected by blood parasites.

  15. Identification of liver protein targets modified by tienilic acid metabolites using a two-dimensional Western blot-mass spectrometry approach

    Science.gov (United States)

    Methogo, Ruth Menque; Dansette, Patrick M.; Klarskov, Klaus

    2007-12-01

    A combined approach based on two-dimensional electrophoresis-immuno-blotting and nanoliquid chromatography coupled on-line with electrospray ionization mass spectrometry (nLC-MS/MS) was used to identify proteins modified by a reactive intermediate of tienilic acid (TA). Liver homogenates from rats exposed to TA were fractionated using ultra centrifugation; four fractions were obtained and subjected to 2D electrophoresis. Following transfer to PVDF membranes, modified proteins were visualized after India ink staining, using an anti-serum raised against TA and ECL detection. Immuno-reactive spots were localized on the PVDF membrane by superposition of the ECL image, protein spots of interest were excised, digested on the membrane with trypsin followed by nLC-MS/MS analysis and protein identification. A total of 15 proteins were identified as likely targets modified by a TA reactive metabolite. These include selenium binding protein 2, senescence marker protein SMP-30, adenosine kinase, Acy1 protein, adenosylhomocysteinase, capping protein (actin filament), protein disulfide isomerase, fumarylacetoacetase, arginase chain A, ketohexokinase, proteasome endopeptidase complex, triosephosphate isomerase, superoxide dismutase, dna-type molecular chaperone hsc73 and malate dehydrogenase.

  16. Immunohistochemical and Western blot analysis of two protein tyrosine phosphatase receptors, R and Z1, in colorectal carcinoma, colon adenoma and normal colon tissues.

    Science.gov (United States)

    Woźniak, Marta; Gamian, Elżbieta; Łaczmańska, Izabela; Sąsiadek, Maria M; Duś-Szachniewicz, Kamila; Ziółkowski, Piotr

    2014-05-01

    Two classes of proteins, namely tyrosine kinases (PTK) and phosphatases (PTP), play an important role in cell proliferation and differentiation, thus leading to an acceleration or inhibition of tumour growth. The role of the above proteins in colorectal carcinoma (CRC) growth is a well-known event. In this study we carried out immunohistochemical and Western blot analysis of colorectal carcinoma, adenoma and normal colon tissue in relation to two protein tyrosine phosphatase receptors, R and Z1. Twenty-five cases of CRC were analyzed and the results were compared with similar data obtained in non-malignant tissues. High expression of both PTP receptors was observed in all examined cases of CRC, adenoma and normal colon tissue in this study. These results are not in line with recently published data, showing that genetic coding for PTPRR and PTPRZ1 were hypermethylated in CRC's. We presume that the protein tyrosine phosphatase overexpression in colorectal carcinoma is not enough to protect from the progression of disease.

  17. Direct measurement of tubulin and bulk message distributions on polysomes of growing, starved and deciliated Tetrahymena using RNA gel blots of sucrose gradients containing acrylamide.

    Science.gov (United States)

    Calzone, F J; Callahan, R; Gorovsky, M A

    1988-10-25

    A method was developed using sucrose gradients containing acrylamide which greatly simplifies the measurement of the polysomal distribution of messages. After centrifugation, the acrylamide was polymerized, forming a "polysome gel". RNA gel blots of polysome gels were used to determine the polysomal distributions of alpha-tubulin and total polyadenylated mRNA in growing, starved (nongrowing) and starved-deciliated Tetrahymena and the number of messages loaded onto polysomes was calculated. These measurements indicated that the translational efficiencies of alpha-tubulin mRNA and total polyadenylated mRNA are largely unaffected when the rates of tubulin and total protein synthesis vary dramatically. Thus, differential regulation of alpha-tubulin mRNA translation initiation does not contribute to the greater than 100-fold induction of tubulin synthesis observed during cilia regeneration and in growing cells. The major translation-level process regulating tubulin synthesis in Tetrahymena appears to be a change in message loading mediated by a non-specific message recruitment or unmasking factor.

  18. Charnockitic magmatism in southern India

    Indian Academy of Sciences (India)

    H M Rajesh; M Santosh

    2004-12-01

    Large charnockite massifs cover a substantial portion of the southern Indian granulite terrain. The older (late Archaean to early Proterozoic) charnockites occur in the northern part and the younger (late Proterozoic) charnockites occur in the southern part of this high-grade terrain. Among these, the older Biligirirangan hill, Shevroy hill and Nilgiri hill massifs are intermediate charnockites, with Pallavaram massif consisting dominantly of felsic charnockites. The charnockite massifs from northern Kerala and Cardamom hill show spatial association of intermediate and felsic charnockites, with the youngest Nagercoil massif consisting of felsic charnockites. Their igneous parentage is evident from a combination of features including field relations, mineralogy, petrography, thermobarometry, as well as distinct chemical features. The southern Indian charnockite massifs show similarity with high-Ba–Sr granitoids, with the tonalitic intermediate charnockites showing similarity with high-Ba–Sr granitoids with low K2O/Na2 ratios, and the felsic charnockites showing similarity with high-Ba–Sr granitoids with high K2O/Na2O ratios. A two-stage model is suggested for the formation of these charnockites. During the first stage there was a period of basalt underplating, with the ponding of alkaline mafic magmas. Partial melting of this mafic lower crust formed the charnockitic magmas. Here emplacement of basalt with low water content would lead to dehydration melting of the lower crust forming intermediate charnockites. Conversely, emplacement of hydrous basalt would result in melting at higher fH2O favoring production of more siliceous felsic charnockites. This model is correlated with two crustal thickening phases in southern India, one related to the accretion of the older crustal blocks on to the Archaean craton to the north and the other probably related to the collision between crustal fragments of East and West Gondwana in a supercontinent framework.

  19. Southern Great Plains Safety Orientation

    Energy Technology Data Exchange (ETDEWEB)

    Schatz, John

    2014-05-01

    Welcome to the Atmospheric Radiation Measurement (ARM) Climate Research Facility (ARM) Southern Great Plains (SGP) site. This U.S. Department of Energy (DOE) site is managed by Argonne National Laboratory (ANL). It is very important that all visitors comply with all DOE and ANL safety requirements, as well as those of the Occupational Safety and Health Administration (OSHA), the National Fire Protection Association, and the U.S. Environmental Protection Agency, and with other requirements as applicable.

  20. Southern Appalachian Regional Seismic Network

    Energy Technology Data Exchange (ETDEWEB)

    Chiu, S.C.C.; Johnston, A.C.; Chiu, J.M. [Memphis State Univ., TN (United States). Center for Earthquake Research and Information

    1994-08-01

    The seismic activity in the southern Appalachian area was monitored by the Southern Appalachian Regional Seismic Network (SARSN) since late 1979 by the Center for Earthquake Research and Information (CERI) at Memphis State University. This network provides good spatial coverage for earthquake locations especially in east Tennessee. The level of activity concentrates more heavily in the Valley and Ridge province of eastern Tennessee, as opposed to the Blue Ridge or Inner Piedmont. The large majority of these events lie between New York - Alabama lineament and the Clingman/Ocoee lineament, magnetic anomalies produced by deep-seated basement structures. Therefore SARSN, even with its wide station spacing, has been able to define the essential first-order seismological characteristics of the Southern Appalachian seismic zone. The focal depths of the southeastern U.S. earthquakes concentrate between 8 and 16 km, occurring principally beneath the Appalachian overthrust. In cross-sectional views, the average seismicity is shallower to the east beneath the Blue Ridge and Piedmont provinces and deeper to the west beneath the Valley and Ridge and the North American craton. Results of recent focal mechanism studies by using the CERI digital earthquake catalog between October, 1986 and December, 1991, indicate that the basement of the Valley and Ridge province is under a horizontal, NE-SW compressive stress. Right-lateral strike-slip faulting on nearly north-south fault planes is preferred because it agrees with the trend of the regional magnetic anomaly pattern.

  1. Atmospheric Chemistry Over Southern Africa

    Science.gov (United States)

    Gatebe, Charles K.; Levy, Robert C.; Thompson, Anne M.

    2011-01-01

    During the southern African dry season, regional haze from mixed industrial pollution, biomass burning aerosol and gases from domestic and grassland fires, and biogenic sources from plants and soils is worsened by a semi-permanent atmosphere gyre over the subcontinent. These factors were a driver of several major international field campaigns in the 1990s and early 2000s, and attracted many scientists to the region. Some researchers were interested in understanding fundamental processes governing chemistry of the atmosphere and interaction with climate change. Others found favorable conditions for evaluating satellite-derived measurements of atmospheric properties and a changing land surface. With that background in mind a workshop on atmospheric chemistry was held in South Africa. Sponsored by the International Commission for Atmospheric Chemistry and Global Pollution (ICACGP; http://www.icacgp.org/), the workshop received generous support from the South African power utility, Eskom, and the Climatology Research Group of the University of the Witwatersrand, Johannesburg, South Africa. The purpose of the workshop was to review some earlier findings as well as more recent findings on southern African climate vulnerability, chemical changes due to urbanization, land-use modification, and how these factors interact. Originally proposed by John Burrows, president of ICACGP, the workshop was the first ICACGP regional workshop to study the interaction of air pollution with global chemical and climate change. Organized locally by the University of the Witwatersrand, the workshop attracted more than 60 delegates from South Africa, Mozambique, Botswana, Zimbabwe, France, Germany, Canada, and the United States. More than 30 presentations were given, exploring both retrospective and prospective aspects of the science. In several talks, attention was focused on southern African chemistry, atmospheric pollution monitoring, and climate processes as they were studied in the field

  2. Detection of mutation in embB gene of Mycobacterium tuberculosis from clinical isolates of tuberculous patients in China by means of reverse-dot blot hybridization

    Institute of Scientific and Technical Information of China (English)

    XUE QIONG WU; YANG LU; JIAN QIN LIANG; JUN XIAN ZHANG; GUANG YU ZHANG; CUI HUAN L(U); HONG MIN LI; BEI CHUAN DING

    2006-01-01

    The relationship between embB mutation of Mycobacterium tuberculosis and ethambutol (EMB) resistance of the clinical isolates of tuberculous patients in China was investigated by reversedot blot hybridization (RDBH) in addition to evaluating the clinical value with application of PCR-RD-BH technique to detect EMB resistance. In the present study, the genotypes of the 258 bp fragments of embB genes from 196 clinical isolates of M. tuberculosis were analysed with RDBH and DNA sequencing. It was demonstrated that 60 out of 91 phenotypically EMB-resistant isolates (65.9%) showed 5 types of missense mutations at codon 306 of embB gene, resulting in the replacement of the Met residue of the wild type strain with Val, Ile or Leu residues. In these mutations, the GTP mutation (38/91,41.8% ) and the ATA mutation ( 16/91, 17.6% ) were the most encountered genotypes. The embB mutation at codon 306 could also be found in 69 isolates of phenotypically EMB-sensitive but resistant to other anti-tuberculous drugs, but no such gene mutation could be found in 36 strains of drug-sensitive isolates. Meanwhile, the concordance with the results of DNA sequencing for one wide-type probe and 5 probes for specific mutations was 100%. It was concluded that the EMB-resistance occurring in most M. tuberculosis is due to appearance of embB mutation at codon 306, and the PCR-RDBH assay was proved to be a rapid, simple and reliable method for the detection of gene mutations, which might be a good alternative for the drug-resistance screening.

  3. A comparative study of Toxoplasma gondii seroprevalence in mink using a modified agglutination test, a Western blot, and enzyme-linked immunosorbent assays.

    Science.gov (United States)

    Gu, Yi; Wang, Zedong; Cai, Yufeng; Li, Xiaoxing; Wei, Feng; Shang, Limin; Li, Jiping; Liu, Quan

    2015-09-01

    Toxoplasma gondii can infect almost all warm-blooded animals, and many serological methods have been developed to detect T. gondii infection in a variety of animal species. In the present study, the seroprevalence of T. gondii infection in farmed mink in northeast China was determined using the modified agglutination test (MAT), a Western blot (WB), and 3 enzyme-linked immunosorbent assays (ELISAs) with protein A/G conjugate, using either of 2 recombinant dense granule antigens, GRA1 and GRA7, or Toxoplasma soluble antigens (TSA). There was no significant difference between the detection results of the GRA1-, GRA7-, and TSA-ELISAs and WB (McNemar chi-square, P > 0.05), but a significant difference was observed between MAT and WB (P < 0.05). A near perfect agreement (97.0%) was found between the GRA7-ELISA and WB (κ = 0.83), and a substantial agreement (92.4-93.1%) was observed in the TSA- and GRA1-ELISAs (κ = 0.68-0.73). The GRA7-ELISA showed the highest sensitivity and specificity, and the lowest false-positive and negative rates, while the MAT gave both a low sensitivity and frequent false positives in comparison to the WB. Receiver operating characteristic analysis revealed the largest area under curve of 0.85 (95% confidence interval: 0.74-0.96), and the highest relative sensitivity (72.7%) and specificity (99.0%) for a cutoff value of 0.19 in the GRA7-ELISA. These results indicate that the GRA7-ELISA is suitable for detection of T. gondii infection in mink and that MAT should be used with caution.

  4. Western blot data using two distinct anti-O-GlcNAc monoclonal antibodies showing unique glycosylation status on cellular proteins under 2-deoxy-d-glucose treatment

    Directory of Open Access Journals (Sweden)

    Tetsuya Okuda

    2017-02-01

    Full Text Available Protein modification by O-linked N-acetylglucosamine (O-GlcNAcylation is one of the post transcriptional modifications occurring on cellular proteins. This paper provides a data set relating to the O-GlcNAcylation of cellular proteins detected by RL2 and CTD110.6 antibodies, which are commonly used for detection of protein O-GlcNAcylation, in 2-deoxy-d-glucose (2DG-treated human teratocarcinoma NCCIT cells in support of the research article entitled “A novel, promoter-based, target-specific assay identifies 2-deoxy-d-glucose as an inhibitor of globotriaosylceramide biosynthesis” (Okuda et al., 2009 [1]. The main article described a suppressive effect of 2DG on an Sp1 target gene in NCCIT cells and discussed the relationship between the effect of 2DG and O-GlcNAcylation status of Sp1. The data in this paper complements this relationship by Western blotting and clearly showed that the 2DG treatment increased O-GlcNAcylation of cellular proteins in NCCIT cells, whereas the RL2 and CTD110.6 epitopes were detected in a different manner. The RL2 epitope was detected on Sp1 during 2DG treatment, and the level was transiently increased at 24 h. In contrast, the CTD110.6 epitope became detectable on Sp1 over 72 h after 2DG treatment, and then the other proteins containing CTD110.6 epitopes also appeared in the cell lysates and the anti-Sp1 antibody precipitates.

  5. HIV-1/2 indeterminate Western blot results: follow-up of asymptomatic blood donors in Belo Horizonte, Minas Gerais, Brazil

    Directory of Open Access Journals (Sweden)

    CARNEIRO-PROIETTI A.B.F.

    1999-01-01

    Full Text Available The clinical and public health importance of indeterminate results in HIV-1/2 testing is still difficult to evaluate in volunteer blood donors. At Fundação Hemominas, HIV-1/2 ELISA is used as the screening test and, if reactive, is followed by Western blot (WB. We have evaluated 84 blood donors who had repeatedly reactive ELISA tests for HIV-1/2, but indeterminate WB results. Sixteen of the 84 donors (19.0% had history of sexually transmitted diseases; 18/84 (21.4% informed receiving or paying for sex; 3/84 (3.6% had homosexual contact; 2/26 women (7.6% had past history of multiple illegal abortions and 3/84 (3.6% had been previously transfused. Four out of 62 donors (6.5% had positive anti-nuclear factor (Hep2, with titles up to 1:640. Parasitological examination of the stool revealed eggs of S. mansoni in 4/62 (6.4% donors and other parasites in 8/62 (12.9%. Five (5.9% of the subjects presented overt seroconversion for HIV-1/2, 43/84 (51.2% had negative results on the last visit, while 36/84 (42.9% remained WB indeterminate. Although some conditions could be found associated with the HIV-1/2 indeterminate WB results and many donors had past of risky behavior, the significance of the majority of the results remains to be determined.

  6. Development of a Rapid Reverse Blot Hybridization Assay for Detection of Clinically Relevant Antibiotic Resistance Genes in Blood Cultures Testing Positive for Gram-Negative Bacteria

    Science.gov (United States)

    Wang, Hye-young; Yoo, Gilsung; Kim, Juwon; Uh, Young; Song, Wonkeun; Kim, Jong Bae; Lee, Hyeyoung

    2017-01-01

    Rapid and accurate identification of the causative pathogens of bloodstream infections is crucial for the prompt initiation of appropriate antimicrobial therapy to decrease the related morbidity and mortality rates. The aim of this study was to evaluate the performance of a newly developed PCR-reverse blot hybridization assay (REBA) for the rapid detection of Gram-negative bacteria (GNB) and their extended-spectrum β-lactamase (ESBL), AmpC β-lactamase, and carbapenemase resistance genes directly from the blood culture bottles. The REBA-EAC (ESBL, AmpC β-lactamase, carbapenemase) assay was performed on 327 isolates that were confirmed to have an ESBL producer phenotype, 200 positive blood culture (PBCs) specimens, and 200 negative blood culture specimens. The concordance rate between the results of REBA-EAC assay and ESBL phenotypic test was 94.2%. The sensitivity, specificity, positive predictive value, and negative predictive value of the REBA-EAC assay for GNB identification in blood culture specimens were 100% (95% CI 0.938–1.000, P < 0.001), 100% (95% CI 0.986–1.000, P < 0.001), 100% (95% CI 0.938–1.000, P < 0.001), and 100% (95% CI 0.986–1.000, P < 0.001), respectively. All 17 EAC-producing GNB isolates from the 73 PBCs were detected by the REBA-EAC assay. The REBA-EAC assay allowed easy differentiation between EAC and non-EAC genes in all isolates. Moreover, the REBA-EAC assay was a rapid and reliable method for identifying GNB and their β-lactamase resistance genes in PBCs. Thus, this assay may provide essential information for accelerating therapeutic decisions to achieve earlier appropriate antibiotic treatment during the acute phase of bloodstream infection. PMID:28232823

  7. Development of a Rapid Reverse Blot Hybridization Assay for Detection of Clinically Relevant Antibiotic Resistance Genes in Blood Cultures Testing Positive for Gram-Negative Bacteria.

    Science.gov (United States)

    Wang, Hye-Young; Yoo, Gilsung; Kim, Juwon; Uh, Young; Song, Wonkeun; Kim, Jong Bae; Lee, Hyeyoung

    2017-01-01

    Rapid and accurate identification of the causative pathogens of bloodstream infections is crucial for the prompt initiation of appropriate antimicrobial therapy to decrease the related morbidity and mortality rates. The aim of this study was to evaluate the performance of a newly developed PCR-reverse blot hybridization assay (REBA) for the rapid detection of Gram-negative bacteria (GNB) and their extended-spectrum β-lactamase (ESBL), AmpC β-lactamase, and carbapenemase resistance genes directly from the blood culture bottles. The REBA-EAC (ESBL, AmpC β-lactamase, carbapenemase) assay was performed on 327 isolates that were confirmed to have an ESBL producer phenotype, 200 positive blood culture (PBCs) specimens, and 200 negative blood culture specimens. The concordance rate between the results of REBA-EAC assay and ESBL phenotypic test was 94.2%. The sensitivity, specificity, positive predictive value, and negative predictive value of the REBA-EAC assay for GNB identification in blood culture specimens were 100% (95% CI 0.938-1.000, P < 0.001), 100% (95% CI 0.986-1.000, P < 0.001), 100% (95% CI 0.938-1.000, P < 0.001), and 100% (95% CI 0.986-1.000, P < 0.001), respectively. All 17 EAC-producing GNB isolates from the 73 PBCs were detected by the REBA-EAC assay. The REBA-EAC assay allowed easy differentiation between EAC and non-EAC genes in all isolates. Moreover, the REBA-EAC assay was a rapid and reliable method for identifying GNB and their β-lactamase resistance genes in PBCs. Thus, this assay may provide essential information for accelerating therapeutic decisions to achieve earlier appropriate antibiotic treatment during the acute phase of bloodstream infection.

  8. Identification of reference proteins for Western blot analyses in mouse model systems of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD toxicity.

    Directory of Open Access Journals (Sweden)

    Stephenie D Prokopec

    Full Text Available Western blotting is a well-established, inexpensive and accurate way of measuring protein content. Because of technical variation between wells, normalization is required for valid interpretation of results across multiple samples. Typically this involves the use of one or more endogenous controls to adjust the measured levels of experimental molecules. Although some endogenous controls are widely used, validation is required for each experimental system. This is critical when studying transcriptional-modulators, such as toxicants like 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD.To address this issue, we examined hepatic tissue from 192 mice representing 47 unique combinations of strain, sex, Ahr-genotype, TCDD dose and treatment time. We examined 7 candidate reference proteins in each animal and assessed consistency of protein abundance through: 1 TCDD-induced fold-difference in protein content from basal levels, 2 inter- and intra- animal stability, and 3 the ability of each candidate to reduce instability of the other candidates. Univariate analyses identified HPRT as the most stable protein. Multivariate analysis indicated that stability generally increased with the number of proteins used, but gains from using >3 proteins were small. Lastly, by comparing these new data to our previous studies of mRNA controls on the same animals, we were able to show that the ideal mRNA and protein control-genes are distinct, and use of only 2-3 proteins provides strong stability, unlike in mRNA studies in the same cohort, where larger control-gene batteries were needed.

  9. Simultaneous detection of Anaplasma and Ehrlichia species in ruminants and detection of Ehrlichia ruminantium in Amblyomma variegatum ticks by reverse line blot hybridization.

    Science.gov (United States)

    Bekker, Cornelis P J; de Vos, Sander; Taoufik, Amar; Sparagano, Olivier A E; Jongejan, Frans

    2002-10-22

    The detection of Anaplasma and Ehrlichia species is usually based on species-specific PCR assays, since no assay is yet available which can detect and identify these species simultaneously. To this end, we developed a reverse line blot (RLB) assay for simultaneous detection and identification of Anaplasma and Ehrlichia species in domestic ruminants and ticks. In a PCR the hypervariable V1 region of the 16S ribosomal RNA (rRNA) gene was amplified with a set of primers unique for members of the genera Anaplasma and Ehrlichia [Int. J. Syst. Evol. Microbiol. 51 (2001) 2145]. Amplified PCR products from blood of domestic ruminants or Amblyomma variegatum tick samples were hybridized onto a membrane to which eight species-specific oligonucleotide probes and one Ehrlichia and Anaplasma catch-all oligonucleotide probe were covalently linked. No DNA was amplified from uninfected blood, nor from other hemoparasites such as Theileria annulata, or Babesia bigemina. The species-specific probes did not cross-react with DNA amplified from other species. E. ruminantium, A. ovis and another Ehrlichia were identified by RLB in blood samples collected from small ruminants in Mozambique. Finally, A. variegatum ticks were tested after feeding on E. ruminantium infected sheep. E. ruminantium could be detected in adult ticks even if feeding of nymphs was carried out 3.5 years post-infection. In conclusion, the developed species-specific oligonucleotide probes used in an RLB assay can simultaneously detect and identify several Ehrlichia and Anaplasma species. However, as no quantitative data for the detection limit are available yet, only positive results are interpretable at this stage.

  10. Geothermal resources of southern Idaho

    Science.gov (United States)

    Mabey, Don R.

    1983-01-01

    The geothermal resource of southern Idaho as assessed by the U.S. Geological Survey in 1978 is large. Most of the known hydrothermal systems in southern Idaho have calculated reservoir temperatures of less than 150?C. Water from many of these systems is valuable for direct heat applications, but is lower than the temperature of interest for commercial generation of electricity at the present time. Most of the known and inferred geothermal resources of southern Idaho underlie the Snake River Plain. However, major uncertainties exist concerning the geology and temperatures beneath the plain. By far the largest hydrothermal system in Idaho is in the Bruneau-Grand View area of the western Snake River Plain with a calculated reservoir temperature of 107?C and an energy of 4.5? 10 20 joules. No evidence of higher temperature water associated with this system has been found. Although the geology of the eastern Snake River Plain suggests that a large thermal anomaly may underlie this area of the plain, direct evidence of high temperatures has not been found. Large volumes of water at temperatures between 90? and 150?C probably exist along the margins of the Snake River Plain and in local areas north and south of the plain. Areas that appear particularly promising for the occurrence of large high-temperature hydrothermal systems are: the area north of the Snake River Plain and west of the Idaho batholith, the Island Park area, segments of the margins of the eastern Snake River Plain, and the Blackfoot lava field.

  11. Cystic echinococcosis in Southern Israel.

    Science.gov (United States)

    Ben-Shimol, Shalom; Sagi, Orli; Houri, Ohad; Bazarsky, Elina; Berkowitz, Anat; Bulkowstein, Shlomi; Barrett, Chiya; Greenberg, David

    2016-01-01

    The aim of this retrospective, population-based study was to characterize demographically and clinically cystic-echinococcosis (CE) in southern Israel, between 2005 and 2012. Newly-diagnosed (nd-CE) and past-diagnosed (pd-CE, diagnosed before the study) cases were defined. Two populations live in southern-Israel, receiving medical treatment at a single hospital: the Jewish and the Bedouin populations (resembling resource-rich and resource-poor populations, respectively). 126 CE cases were identified; 55 nd-CE and 71 pd-CE. Mean annual nd-CE incidence per 100,000 in the Bedouin and Jewish populations were 2.7 ± 1.2 and 0.4 ± 0.3, respectively (Pborn outside Israel. Liver and lung involvement were recorded in 85.7% and 15.1% of overall-CE, respectively. Abdominal pain, cough, fever, eosinophilia and asymptomatic disease were documented in 63.6%, 32.7%, 27.3%, 41.5% and 12.7% of nd-CE, respectively. Serology sensitivity for first test and any positive test were 67.3% and 83.3%, respectively. Computed tomography, ultrasonography and X-ray diagnosis were documented in 79.2%, 58.4% and 17.0% of overall-CE, respectively, with ultrasonography mainly used in liver-CE and X-ray in lung-CE. Treatment included surgery and albendazole in 50.0% and 55.3% of CE, respectively. We conclude that CE is endemic in southern-Israel among the Bedouin population, while disease is probably mainly imported in the Jewish population. Liver involvement and eosinophilia rates were high compared with those of other endemic regions, possibly due to differences in the timing of diagnosis. These findings may help developing treatment and prevention strategies.

  12. Evaluación de la técnica Western blot para la detección de antígenos de Hymenolepis nana

    Directory of Open Access Journals (Sweden)

    Flora Chávez-Salas

    2013-04-01

    Full Text Available Este trabajo tuvo como objetivo evaluar la técnica de inmunoelectrotransferencia (Western Blot para detectar los antígenos específicos de excreción/secreción de Hymenolepis nana en sueros de pacientes con himenolepiosis y con otras helmintiosis confirmadas. Se utilizó a Mesocricetus auratus “hamster” para obtener ejemplares adultos de H. nana. Los antígenos de excreción/secreción fueron obtenidos en el medio MEM (Minimum Essential Medium Eagle, y enfrentados con un grupo de sueros de pacientes con himenolepiosis confirmada para evaluar su calidad inmunológica y con sueros individuales de pacientes con himenolepiosis y con otras helmintiosis confirmadas para detectar mediante la técnica de “Western Blot”, los antígenos específicos de este cestode. El grupo de sueros de pacientes con himenolepiosis confirmada reconoció las bandas antigénicas de 50,1; 42,6; 38,9; 32,9; 26,3; 22,4 y 18,6 kDa; sin embargo, los sueros individuales reconocieron diferente número de bandas, siendo la de 50,1 KDa la que fue reconocida por todos ellos. Los sueros de pacientes con helmintiosis confirmadas no reconocieron la banda de 50,1 kDa; sin embargo, dieron reacción cruzada con algunas de las demás bandas, a excepción de los sueros de pacientes con cisticercosis que no reconocieron a ninguna de las bandas de estos antígenos. Se concluye que el antígeno de excreción/secreción de H. nana de 50,1 kDa es específico de este cestode por ser reconocido por todos los sueros de pacientes con himenolepiosis confirmada y no por sueros de pacientes con otras helmintiosis utilizando la técnica de “Western Blot”.

  13. The Southern Sky Redshift Survey

    Science.gov (United States)

    da Costa, L. Nicolaci; Willmer, C. N. A.; Pellegrini, P. S.; Chaves, O. L.; Rité, C.; Maia, M. A. G.; Geller, M. J.; Latham, D. W.; Kurtz, M. J.; Huchra, J. P.; Ramella, M.; Fairall, A. P.; Smith, C.; Lípari, S.

    1998-07-01

    We report redshifts, magnitudes, and morphological classifications for 5369 galaxies with m_B Complejo Astronomico El Leoncito, operated under agreement between the Consejo Nacional de Investigaciones Científicas de la República Argentina and the National Universities of La Plata, Córdoba, and San Juan; the European Southern Observatory, La Silla, Chile, partially under the bilateral ESO-Observatório Nacional agreement; Fred Lawrence Whipple Observatory; Laboratório Nacional de Astrofísica, Brazil; and the South African Astronomical Observatory.

  14. Rural migration in southern Nevada

    Energy Technology Data Exchange (ETDEWEB)

    Mosser, D.; Soden, D.L.

    1993-08-01

    This study reviews the history of migration in two rural counties in Southern Nevada. It is part of a larger study about the impact of a proposed high-level nuclear waste repository on in- and out-migration patterns in the state. The historical record suggests a boom and bust economic cycle has predominated in the region for the past century creating conditions that should be taken into account by decision makers when ascertaining the long-term impacts of the proposed repository.

  15. A comparison of the immune parameters of dogs infected with visceral leishmaniasis using Western blot and neutralization techniques Comparação dos parâmetros imunológicos de cães infectados com leishmaniose visceral usando as técnicas de Western blot e neutralização

    Directory of Open Access Journals (Sweden)

    Yeda L. Nogueira

    2007-12-01

    Full Text Available The Western blot technique was used to demonstrate the presence of antibodies in the blood of dogs that presented canine visceral leishmaniasis. This technique was used against some specific molecules present in the lysate of the promastigote form of Leshmania chagasi.Through the association of the results of the Western blot technique with the morphological alterations seen as a result of the serum neutralization technique performed in McCoy cells (which mimetizes the macrophage it was possible to observe the role of some molecules of great relevance in determining the disease in symptomatic dogs as well as that of some other molecules associated with asymptomatic infected dogs that may become transmitters as well as differentiating them as asymptomatic resistant dogs. In the sera analyses carried out during the immunobloting a variation of 9 to 27 immunoreacting bands was observed, which were then compared using Dice's similarity coefficient. In the dendrogram constructed on the basis of the coefficient, 50% similarity was observed among the total number of reagent bands with the promastigote lysate, thus creating five groups. The main difference observed related to the clinical condition of the dogs: symptomatic and asymptomatic dogs were found in separate groups. The asymptomatic group of dogs was distributed in two different places in the dendrogram because they presented two different behavior patterns regarding the cellular morphology in the serum neutralization reaction: the presence or absence of cellular lysis. According to this analysis it is possible to evaluate the immune status and associate it with specific markers observed in the reaction found in the Western blot strips.A técnica de Western blot foi utilizada para demonstrar a presença de anticorpos do soro de cães, que apresentavam leishmaniose visceral canina, contra algumas moléculas específicas no lisado da forma promastigota de Leshmania chagasi.Através da associa

  16. Southern Africa CTA Site Proposal

    CERN Document Server

    Krüger, P P

    2012-01-01

    Southern Africa has some of the world's best sites for air Cherenkov telescopes. South Africa has only one viable site, which is south of Sutherland and also close to the Southern African Large Telescope (SALT). This site has very good infrastructure and is easy to access, but only 47% of the night-time has a cloudless sky usable for observations. Namibia, which already hosts the H.E.S.S telescope, has a number of potential sites with much less cloud coverage. The H.E.S.S. site is one of the highest of these sites at 1840 m a.s.l. with about 64% of the night-time cloudless. It also has very low night sky background levels and is relatively close (about 100 km) to Windhoek. Moving further away from Windhoek to the south, the cloud coverage and artificial night sky brightness becomes even less, with the site at Kuibis (between Keetmanshoop and Luderitz) at 1640 m a.s.l. having clear night skies 73% of the time. Even though this site seems remote (being 660 km from Windhoek by road), it is close to the national ...

  17. Atmospheric chemistry over southern Africa

    Science.gov (United States)

    Gatebe, Charles K.; Levy, Robert C.; Thompson, Anne M.

    2012-03-01

    Changing Chemistry in a Changing Climate: Human and Natural Impacts Over Southern Africa (C4-SAR); Midrand, South Africa, 31 May to 3 June 2011 During the southern African dry season, regional haze from mixed industrial pollution, biomass burning aerosol and gases from domestic and grassland fires, and biogenic sources from plants and soils is worsened by a semipermanent atmospheric gyre over the subcontinent. These factors were a driver of several major international field campaigns in the 1990s and early 2000s and attracted many scientists to the region. Some researchers were interested in understanding fundamental processes governing chemistry of the atmosphere and interaction with climate change. Others found favorable conditions for evaluating satellite- derived measurements of atmospheric properties and a changing land surface. With that background in mind a workshop on atmospheric chemistry was held in South Africa. Sponsored by the International Commission on Atmospheric Chemistry and Global Pollution (ICACGP; http://www.icacgp.org/), the workshop received generous support from Eskom, the South African power utility; and the Climatology Research Group of the University of the Witwatersrand, Johannesburg, South Africa.

  18. Evaluación de las pruebas dot blot y aglutinación de látex para el diagnóstico de cisticercosis en Perú

    Directory of Open Access Journals (Sweden)

    Eduardo Miranda-Ulloa

    2014-06-01

    Full Text Available Con el objetivo de evaluar las pruebas dot blot y aglutinación de látex para la detección de cisticercosis humana con antígeno de líquido de cisticerco de Taenia solium, se usaron 125 sueros humanos, de los cuales 60 procedían de personas con cisticercosis confirmada por Western Blot, 45 de personas con otras enfermedades parasitarias y 20 de personas aparentemente sanas. La concentración óptima del antígeno para impregnar las tiras dot blot fue de 0,01 ug/uL, y para impregnar las partículas de látex fue de 0,092 ug/uL. Para la prueba dot blot se encontró una sensibilidad del 100% y especificidad del 87,7%; para la aglutinación de látex una sensibilidad del 93,3% y especificidad del 89,2%. Ambas pruebas podrían ser de utilidad y factibles de implementar como alternativas de diagnóstico serológico en laboratorios de áreas endémicas del Perú

  19. Evaluación de las pruebas dot blot y aglutinación de látex para el diagnóstico de cisticercosis en Perú

    Directory of Open Access Journals (Sweden)

    Eduardo Miranda-Ulloa

    Full Text Available Con el objetivo de evaluar las pruebas dot blot y aglutinación de látex para la detección de cisticercosis humana con antígeno de líquido de cisticerco de Taenia solium, se usaron 125 sueros humanos, de los cuales 60 procedían de personas con cisticercosis confirmada por Western Blot, 45 de personas con otras enfermedades parasitarias y 20 de personas aparentemente sanas. La concentración óptima del antígeno para impregnar las tiras dot blot fue de 0,01 ug/uL, y para impregnar las partículas de látex fue de 0,092 ug/uL. Para la prueba dot blot se encontró una sensibilidad del 100% y especificidad del 87,7%; para la aglutinación de látex una sensibilidad del 93,3% y especificidad del 89,2%. Ambas pruebas podrían ser de utilidad y factibles de implementar como alternativas de diagnóstico serológico en laboratorios de áreas endémicas del Perú

  20. The Southern Alberta Information Resources (SAIR Project

    Directory of Open Access Journals (Sweden)

    Kathy Crewdson

    2008-06-01

    Full Text Available The Southern Alberta Information Resources (SAIR Project is a collaborative online bibliography of published resources significant to southern Alberta. This paper introduces the partners, briefly summarizes the purpose of the project, describes the progress and challenges encountered thus far, and discusses the intended project outcomes and impacts.

  1. New Moho map of southern Norway

    DEFF Research Database (Denmark)

    Stratford, Wanda Rose; Thybo, Hans; Faleide, J.-I.

    2009-01-01

    A recent seismic refraction study across southern Norway has revealed that the up to 2469 m high Southern Scandes Mountains are not isostatically compensated by a thick crust. Rather, the Moho depths are close to average for continental crust with elevations of ~1 km. Evidence from new seismic data...

  2. Southern Energy Efficiency Center (SEEC)

    Energy Technology Data Exchange (ETDEWEB)

    Vieira, Robin; Sonne, Jeffrey; Withers, Charles; Cummings, James; Verdict, Malcolm; Roberts, Sydney

    2009-09-30

    The Southern Energy Efficiency Center (SEEC) builds collaborative partnerships with: state and local governments and their program support offices, the building delivery industry (designers, contractors, realtors and commissioning agents), product manufacturers and their supply chains, utilities and their program implementers, consumers and other stakeholders in order to forge a strong regional network of building energy efficiency allies. Through a project Steering Committee composed of the state energy offices and building industry stakeholders, the SEEC works to establish consensus-based goals, priorities and strategies at the regional, state and local levels that will materially advance the deployment of high-performance “beyond code” buildings. In its first Phase, SEEC will provide limited technical and policy support assistance, training, certification and education to a wide spectrum of the building construction, codes and standards, and the consumer marketplace.

  3. Southern company energy storage study :

    Energy Technology Data Exchange (ETDEWEB)

    Ellison, James; Bhatnagar, Dhruv; Black, Clifton; Jenkins, Kip

    2013-03-01

    This study evaluates the business case for additional bulk electric energy storage in the Southern Company service territory for the year 2020. The model was used to examine how system operations are likely to change as additional storage is added. The storage resources were allowed to provide energy time shift, regulation reserve, and spinning reserve services. Several storage facilities, including pumped hydroelectric systems, flywheels, and bulk-scale batteries, were considered. These scenarios were tested against a range of sensitivities: three different natural gas price assumptions, a 15% decrease in coal-fired generation capacity, and a high renewable penetration (10% of total generation from wind energy). Only in the elevated natural gas price sensitivities did some of the additional bulk-scale storage projects appear justifiable on the basis of projected production cost savings. Enabling existing peak shaving hydroelectric plants to provide regulation and spinning reserve, however, is likely to provide savings that justify the project cost even at anticipated natural gas price levels. Transmission and distribution applications of storage were not examined in this study. Allowing new storage facilities to serve both bulk grid and transmission/distribution-level needs may provide for increased benefit streams, and thus make a stronger business case for additional storage.

  4. Comparison of Linear Array and Line Blot Assay for Detection of Human Papillomavirus and Diagnosis of Cervical Precancer and Cancer in the Atypical Squamous Cell of Undetermined Significance and Low-Grade Squamous Intraepithelial Lesion Triage Study▿

    OpenAIRE

    Castle, Philip E.; Gravitt, Patti E.; Solomon, Diane; Wheeler, Cosette M.; Schiffman, Mark

    2007-01-01

    We evaluated Linear Array (LA), a newly commercialized PGMY09/11 L1 consensus primer PCR test that detects 37 human papillomavirus (HPV) genotypes by reverse line blot hybridization, for the detection of individual HPV genotypes and carcinogenic HPV and its clinical performance for detecting 2-year cumulative cervical precancer and cancer using archived specimens from the Atypical Squamous Cell of Undetermined Significance (ASCUS) and Low-Grade Squamous Intraepithelial Lesion Triage Study. LA...

  5. Waterfowl breeding population survey: Southern Manitoba: 1983

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — This report summarizes the Waterfowl Breeding Population and Habitat Survey for southern Manitoba during 1983. The primary purpose of the survey is to provide...

  6. Marine Invertebrate assemblages in southern California

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This is a point file of invertebrate site clusters calculated from benthic trawls completed by the Southern California Coastal Water Research Project (SCCWRP). Data...

  7. Fish assemblages in southern California kelp forests.

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This is a point file of fish assemblages calculated from diver surveys in kelp forests in Southern California. Visual census data was combined for two separate...

  8. Training traditional birth attendants in southern Sudan.

    Science.gov (United States)

    Haarsager, Mary

    2008-01-01

    Traditional birth attendants are currently the principal service providers to pregnant women in southern Sudan. A training program provides education to promote maternal and newborn health as well as birth preparedness and establishes mechanisms for supportive supervision.

  9. Southern Watersheds Common Reedgrass Project Progress Report

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — The Southern Watersheds includes the drainages of the Northwest River, the North Landing River, and Back Bay in the southeastern corner of Virginia. Common reedgrass...

  10. Census summary of southern sea otter 2016

    Data.gov (United States)

    U.S. Geological Survey, Department of the Interior — The GIS shapefile "Census summary of southern sea otter 2016" provides a standardized tool for examining spatial patterns in abundance and demographic trends of the...

  11. Climate Prediction Center Southern Oscillation Index

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This is one of the CPC?s Monthly Atmospheric and Sea Surface Temperature (SST)Indices. It contains Southern Oscillation Index which is standardized sea level...

  12. Waterfowl production survey: July 1997: Southern Manitoba

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — This report summarizes the Waterfowl Production and Habitat Survey for southern Manitoba during 1997. The primary purpose of the survey is to provide information on...

  13. Waterfowl production survey: Southern Alberta: 1981

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — This report summarizes the Waterfowl Production and Habitat Survey for southern Alberta during 1981. The primary purpose of the survey is to provide information on...

  14. Waterfowl breeding pair survey: Southern Alberta: 1981

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — This report summarizes the Waterfowl Breeding Population and Habitat Survey for southern Alberta during 1981. The primary purpose of the survey is to provide...

  15. Experimental late brood surveys: Southern Saskatchewan: 1991

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — This report summarizes the late brood surveys for southern Saskatchewan during 1991. Survey methods, weather and habitat conditions, production indices, and tables...

  16. Nearshore marine fish assemblages in southern California

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Fish benthic trawls were completed by the Southern California Coastal Water Research Project (SCCWRP). Data from 425 fisheries independent trawls ranging from 2-215...

  17. Southern Watersheds Common Reedgrass Monitoring Plan

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — The Southern Watersheds Common Reedgrass Project is an interagency effort to increase public awareness of the common reedgrass problem, demonstrate effective control...

  18. Arctic and Southern Ocean Sea Ice Concentrations

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Monthly sea ice concentration for Arctic (1901 to 1995) and Southern oceans (1973 to 1990) were digitized on a standard 1-degree grid (cylindrical projection) to...

  19. Star forming regions of the southern galaxy

    Science.gov (United States)

    Kuiper, T. B. H.; Er, J. W., the molecular gas, a

    1987-01-01

    A catalog of southern dust cloud properties is being compiled to aid in the planning and analysis of radio spectral line surveys in the southern hemisphere. Ultimately, images of dust temperature and column density will be produced. For the interim, a list of the 60 and 100 micron fluxes was prepared for the cores and adjacent backgrounds of 65 prominent dust clouds. Dust temperatures and column densities were derived.

  20. 77 FR 17122 - Indiana Southern Railroad, LLC-Temporary Trackage Rights Exemption-Norfolk Southern Railway Company

    Science.gov (United States)

    2012-03-23

    ... Surface Transportation Board Indiana Southern Railroad, LLC--Temporary Trackage Rights Exemption--Norfolk Southern Railway Company Norfolk Southern Railway Company (NSR), pursuant to a written trackage rights agreement (Agreement), has agreed to grant overhead temporary trackage rights to Indiana Southern...

  1. The Southern Ocean: Source and sink?

    Science.gov (United States)

    Strugnell, J. M.; Cherel, Y.; Cooke, I. R.; Gleadall, I. G.; Hochberg, F. G.; Ibáñez, C. M.; Jorgensen, E.; Laptikhovsky, V. V.; Linse, K.; Norman, M.; Vecchione, M.; Voight, J. R.; Allcock, A. L.

    2011-03-01

    Many members of the benthic fauna of the Antarctic continental shelf share close phylogenetic relationships to the deep-sea fauna adjacent to Antarctica and in other ocean basins. It has been suggested that connections between the Southern Ocean and the deep sea have been facilitated by the presence of a deep Antarctic continental shelf coupled with submerging Antarctic bottom water and emerging circumpolar deep water. These conditions may have allowed 'polar submergence', whereby shallow Southern Ocean fauna have colonised the deep sea and 'polar emergence', whereby deep-sea fauna colonised the shallow Southern Ocean. A recent molecular study showed that a lineage of deep-sea and Southern Ocean octopuses with a uniserial sucker arrangement on their arms appear to have arisen via polar submergence. A distantly related clade of octopuses with a biserial sucker arrangement on their arms (historically placed in the genus Benthoctopus) is also present in the deep-sea basins of the world and the Southern Ocean. To date their evolutionary history has not been examined. The present study investigated the origins of this group using 3133 base pairs (bp) of nucleotide data from five mitochondrial genes (12S rRNA, 16S rRNA, cytochrome c oxidase subunit I, cytochrome c oxidase subunit III, cytochrome b) and the nuclear gene rhodopsin from at least 18 species (and 7 outgroup taxa). Bayesian relaxed clock analyses showed that Benthoctopus species with a high-latitude distribution in the Southern Hemisphere represent a paraphyletic group comprised of three independent clades. The results suggest that the Benthoctopus clade originated in relatively shallow Northern Hemisphere waters. Benthoctopus species distributed in the Southern Ocean are representative of polar emergence and occur at shallower depths than non-polar Benthoctopus species.

  2. Emigration dynamics in southern Africa.

    Science.gov (United States)

    Milazi, D

    1995-01-01

    This review of the dynamics of international migration in Southern Africa focuses on four aspects of labor migration: 1) while migrant workers suffer from discrimination and lack of protection, there are few alternatives for them; 2) the regulations imposed by the Chamber of Mines in South Africa favor the mining industry at the expense of the workers; 3) worker supplier states have few options for negotiating a commercialized migration policy to achieve economic benefits; and 4) foreign mine workers must unionize in order to escape perpetual subordination. The review opens with a consideration of how migrant mine workers from Botswana, Lesotho, and Swaziland have provided a source of cheap labor which has enhanced the economic prosperity of South Africa. The role of the Chamber of Mines in regulating the supply of labor and employment policy for its members is described. Attention is then turned to Lesotho where land pressure has exacerbated poverty levels. Large-scale migration has led the citizens of Lesotho to consider it a place to live or retire to, not a place to work. Labor migration from Lesotho is organized, is supported by the government, is recurrent, and remains a viable alternative despite faltering demand. The discussion of Lesotho includes a consideration of its political, economic, and demographic situation as well as of ecological factors. Briefer analyses are then provided for Botswana, Swaziland, and Mozambique. The receiving country, South Africa, is shown to be suffering a decline in economic growth which is marked by widespread unemployment. More than 250,000 Whites are prospective emigrants from South Africa. After considering the issues surrounding refugees, regional concerns created by changing economic and political scenarios, and labor strategies which could be adopted by supplier states, the report reiterates a series of recommendations which arose from two major conferences on the problem of unemployment. It is concluded that the

  3. The Southern Alberta Information Resources (SAIR Project

    Directory of Open Access Journals (Sweden)

    Kathy Crewdson

    2008-06-01

    Full Text Available Abstract Southern Alberta Information Resources (SAIR is a collaborative bibliography of published resources significant to southern Alberta. Objectives and progress with evolving methodology, technology, issues and challenges are explored within the context of the library field. We investigate a collaborative digital library that allows librarians and non-librarians alike to share information on specific topics through MARC records. An outcome of a collaborative digital library is how to create and sustain interest within the library community. Southern Alberta region was selected based on the authors’ familiarity with the region. Some issues and questions remain to be resolved. Digital formats present a number of challenges in terms of selection and presentation. Legal issues relating to technology such as linking and location information have emerged. Basic technical issues remain, such as, how best to update links.

  4. Indirect taxes on food in Southern Brazil

    Directory of Open Access Journals (Sweden)

    Denize Mirian da Silva

    2013-12-01

    Full Text Available The objective of this paper is to estimate the indirect tax burden on food for ten income classes, based on income and household total expenditure in southern Brazil. Thus it can be seen as indirect taxes on foods affect the monetary income and consumption pattern of households. To reach the objectives proposed, will be used the Pintos-Payeras (2008 model. The database iscomposed by microdata from the Household Budgeting Survey (POF 2008-2009 and the tax regulations of the country and the southern states of Brazil. The results show that indirect taxes on food in Southern Brazil is regressive when based on income and expenditure of household , ie , the poorest people pay proportionately more taxes and have their consumption pattern highest taxed ICMS (Brazilian value added tax is the tax that contributes most to the regressivity.

  5. Matrix-assisted laser desorption-ionization mass spectrometry peptide mass fingerprinting for proteome analysis: identification efficiency after on-blot or in-gel digestion with and without desalting procedures.

    Science.gov (United States)

    Lamer, S; Jungblut, P R

    2001-03-10

    In theory, peptide mass fingerprinting by matrix assisted laser desorption-ionization mass spectrometry (MALDI-MS) has the potential to identify all of the proteins detected by silver staining on gels. In practice, if the genome of the organism investigated is completely sequenced, using current techniques, all proteins stained by Coomassie Brilliant Blue can be identified. This loss of identification sensitivity of ten to hundred-fold is caused by loss of peptides by surface contacts. Therefore, we performed digestion and transfer of peptides in the lower microl range and reduced the number of steps. The peptide mix obtained from in-gel or on-blot digestion was analyzed directly after digestion or after concentration on POROS R2 beads. Eight protein spots of a 2-DE gel from Mycobacterium bovis BCG were identified using these four preparation procedures for MALDI-MS. Overall, on-blot digestion was as effective as in-gel digestion. Whereas higher signal intensities resulted after concentration, hydrophilic peptides are better detected by direct measurement of the peptide mix without POROS R2 concentration.

  6. Southern Fireworks above ESO Telescopes

    Science.gov (United States)

    1999-05-01

    New Insights from Observations of Mysterious Gamma-Ray Burst International teams of astronomers are now busy working on new and exciting data obtained during the last week with telescopes at the European Southern Observatory (ESO). Their object of study is the remnant of a mysterious cosmic explosion far out in space, first detected as a gigantic outburst of gamma rays on May 10. Gamma-Ray Bursters (GRBs) are brief flashes of very energetic radiation - they represent by far the most powerful type of explosion known in the Universe and their afterglow in optical light can be 10 million times brighter than the brightest supernovae [1]. The May 10 event ranks among the brightest one hundred of the over 2500 GRB's detected in the last decade. The new observations include detailed images and spectra from the VLT 8.2-m ANTU (UT1) telescope at Paranal, obtained at short notice during a special Target of Opportunity programme. This happened just over one month after that powerful telescope entered into regular service and demonstrates its great potential for exciting science. In particular, in an observational first, the VLT measured linear polarization of the light from the optical counterpart, indicating for the first time that synchrotron radiation is involved . It also determined a staggering distance of more than 7,000 million light-years to this GRB . The astronomers are optimistic that the extensive observations will help them to better understand the true nature of such a dramatic event and thus to bring them nearer to the solution of one of the greatest riddles of modern astrophysics. A prime example of international collaboration The present story is about important new results at the front-line of current research. At the same time, it is also a fine illustration of a successful collaboration among several international teams of astronomers and the very effective way modern science functions. It began on May 10, at 08:49 hrs Universal Time (UT), when the Burst

  7. Field guide to trees of Southern Africa

    Directory of Open Access Journals (Sweden)

    Rishan Singh

    2012-09-01

    Full Text Available The mainland region of Africa is Southern Africa because it is considered to be robust with an estimate of around 1700 tree species that are native and a couple 100 more that are alien, but have become accustomed to the natural environment; invading, penetrating and replacing vegetation.

  8. Epidemiology of cerebral palsy in Southern Denmark

    DEFF Research Database (Denmark)

    Frøslev-Friis, Christina; Dunkhase-Heinl, Ulrike; Andersen, Johnny Dohn Holmgren;

    2015-01-01

    INTRODUCTION: The aim of this study was to describe the prevalence, subtypes, severity and neuroimaging findings of cerebral palsy (CP) in a cohort of children born in Southern Denmark. Risk factors were analysed and aetiology considered. METHODS: A population-based cohort study covering 17,580 l...

  9. Southern African species of Mentha L. (Lamiaceae

    Directory of Open Access Journals (Sweden)

    L. E. Codd

    1983-12-01

    Full Text Available The species of Mentha L. occurring in Southern Africa are reviewed and a key is provided to two indigenous and one naturalized species.  M. wissii Launert is reduced to M. longifolia (L. Huds. subsp. wissii (Launert Codd.

  10. Faulkner's Southern belle - myth or reality?

    Directory of Open Access Journals (Sweden)

    Nataša Intihar Klančar

    2011-12-01

    Full Text Available The article deals with heroines of William Faulkner's novels Light in August, Absalom, Absalom!, The Sound and the Fury, The Unvanquished, The Town and his short story "A Rose for Emily". The Southern belle features as a recurring character in Faulkner's fiction, her fragility, modesty, weakness yet strength, beauty, sincerity, generous nature, status and her fall from innocence comprise her central characteristics. Confronted with various expectations of Southern society and with the hardships of war, the belle is faced with many obstacles and challenges. Faulkner's heroines face a wide array of problems that prevent them from being and/or remaining a Southern belle. Let us name a few: Lena's inappropriate social status, Joanna's wrong roots, Mrs. Hightower's inability to fulfill her duties as the minister's wife, Ellen's miserable marriage, Judith's sad love life, Rosa's feelings of inferiority and humiliation, Mrs. Compson's failure as a mother, Caddy's weak rebellion against male convention, Drusilla's male characteristics, Linda's unrequited love and Emily's dark secret, to name a few. Through these characters and their destinies Faulkner shows a decaying South whose position has changed considerably over the years. Can the Southern belle save it? Can she save herself?

  11. Dissolved aluminium in the Southern Ocean

    NARCIS (Netherlands)

    Middag, R.; van Slooten, C.; de Baar, H. J. W.; Laan, P.

    2011-01-01

    Dissolved aluminium (Al) occurs in a wide range of concentrations in the world oceans. The concentrations of Al in the Southern Ocean are among the lowest ever observed. An all-titanium CTD sampling system makes it possible to study complete deep ocean sections of Al and other trace elements with th

  12. Marketing University Education: The Southern African Experience.

    Science.gov (United States)

    Maringe, Felix; Foskett, Nick

    2002-01-01

    Examined the perceptions of university marketers in southern Africa. Found a varying awareness of the significance of marketing, with more mature institutions exhibiting more developed marketing orientations. Strategies ranged from marketing as public relations to marketing as sales, with universities in South Africa the only ones demonstrating a…

  13. On some birds from southern Mexico

    NARCIS (Netherlands)

    Mees, G.F.

    1970-01-01

    In the years 1962/64 our museum purchased from Mr. Otto Epping, now of Pittsburgh, U.S.A., a collection of 700 bird-specimens from southern Mexico (mainly from Vera Cruz and Oaxaca, a few specimens from Puebla). As our museum was poorly provided with material from Mexico, this was a very welcome add

  14. Groundwater use on southern Idaho dairies

    Science.gov (United States)

    Dairy production has expanded in irrigated areas of the western and southwestern US, potentially competing for limited water supplies. Groundwater withdrawal was measured for two years on six dairy farms with 660 to 6400 milk cows in southern Idaho. Groundwater withdrawal was calculated on an equiva...

  15. Development of Southern Cross University College

    Science.gov (United States)

    MacGillivray, W. R.

    2013-01-01

    Southern Cross University (SCU) has established a pathways college to increase access to and widen participation in higher education for people in regional areas of Australia. While many Australian universities have preparatory colleges associated with them, SCU College has been designed to make it unique in the sector. SCU College will operate…

  16. The lithospheric mantle below southern West Greenland

    DEFF Research Database (Denmark)

    Sand, Karina Krarup; Waight, Tod Earle; Pearson, D. Graham

    2009-01-01

    Geothermobarometry of primarily garnet lherzolitic xenoliths from several localities in southern West Greenland is applied to address the diamond potential, pressure and temperature distribution and the stratigraphy of the subcontinental lithospheric mantle ~600 Ma ago. The samples are from kimbe...... into the reworked Archean North of the Naqssugtoqidian deformation front....

  17. Plasmodium falciparum Malaria, Southern Algeria, 2007

    OpenAIRE

    Boubidi, Saïd C; Gassen, Ibrahim; Khechache, Yacine; Lamali, Karima; Tchicha, Boualem; Brengues, Cécile; Menegon, Michela; Severini, Carlo; Fontenille, Didier; Harrat, Zoubir

    2010-01-01

    An outbreak of Plasmodium falciparum malaria occurred in Tinzaouatine in southern Algeria in 2007. The likely vector, Anopheles gambiae mosquitoes, had not been detected in Algeria. Genes for resistance to chloroquine were detected in the parasite. The outbreak shows the potential for an increase in malaria vectors in Algeria.

  18. Morphology of Southern Hemisphere Riometer Auroral Absorption

    Science.gov (United States)

    2006-06-01

    range of frequencies used an inverse -square frequency dependence approximately holds. Morphology of Southern Hemisphere Riometer Auroral Absorption...Tecnológico under Proyecto No 1940934. Annex The percentage probability, QA, that an absorption A dB is exceed, is given as: Where Am is

  19. Getting southern Sudanese children to school

    Directory of Open Access Journals (Sweden)

    Sibeso Luswata

    2006-07-01

    Full Text Available The Government of Southern Sudan’s Go to School Initiative,supported by UNICEF, which seeks to get 1.6 millionchildren back in school by the end of 2007, incorporateskey elements of the INEE Minimum Standards for Educationin Emergencies, Chronic Crises and Early Reconstruction.

  20. Linguistic Landscapes in Southern Carinthia (Austria)

    Science.gov (United States)

    Rasinger, Sebastian M.

    2014-01-01

    This paper explores the linguistic landscape (LL) in the southern Austrian province of Carinthia, which is home to an autochthonous Slovene minority. Following several decades of political and legal debate known as the "Ortstafelstreit" ("dispute of topographic signs"), recent legislation has strengthened the status of Slovene…

  1. Economics of southern growth. [7 papers

    Energy Technology Data Exchange (ETDEWEB)

    Liner, E.B.; Lynch, L.K. (eds.)

    1977-01-01

    The book presents both the documentation of Southern economic growth and an examination of the major economic problems. Chapter 1, by Lawrence K. Lynch and E. Evan Brunson, documents Southern growth from 1970 to 1975, compares that growth to growth in the nation, and evaluates the comparative impact of the 1974 to 1975 recession. Chapter 2, by William H. Miernyk, analyzes the structure of the Southern economy in terms of the Clark-Fisher hypothesis. The economic structure of the South has become similar to the nation's; the relative importance of agricultural and energy production in the South will tend to maintain the South's growth advantage. Chapter 3, by Charles F. Haywood, explores the capital needs of the South, and finds that demands for capital will accelerate. Significant inflows of capital from other regions, other nations, and strengthened local sources of capital will be needed. Chapter 4, by Madelyn Lockhart, compares the income distributions and labor markets of the South and the nation, but argues that measures of economic well-being do not properly reflect all factors. Chapter 5, by Russell L. Howard, examines the limitations on area and regional growth imposed by energy prices and availability; he points out that Southern industries are energy-intensive. Chapter 6, by Mancur Olson, proposes a view on why the South is outgrowing the North: that the development of organized interest groups that obtain political and economic power tends to slow the rate of economic growth. Chapter 7, by C. L. Jusenius and L. C. Ledebur, presents comparative data on economic growth and status for the Southern and the Northern tiers of states. Although economic growth in the South has been more rapid in recent years, the economic status of the South still lags the North.

  2. Comparisons of invasive plants in southern Africa originating from southern temperate, northern temperate and tropical regions

    Directory of Open Access Journals (Sweden)

    L. Henderson

    2006-12-01

    Full Text Available A subset of invasive alien plant species in southern Africa was analysed in terms of their history of introduction, rate of spread, countries/region of origin, taxonomy, growth forms, cultivated uses, weed status and current distribution in southern Africa, and comparisons made of those originating from south of the tropic of Capricorn, north of the tropic of Cancer and from the tropics. The subset of 233 species, belonging to 58 families, includes all important declared species and some potentially important species. Almost as many species originate from temperate regions (112 as from the tropics (121. Most southern temperate species came from Australia (28/36, most tropical species from tropical America (92/121 and most northern temperate species from Europe (including the Mediterranean and Asia (58/76. Transformers account for 33% of  all species. More transformers are of tropical origin (36 than of northern temperate (24 and southern temperate origin (18. However. 50% of southern temperate species are transformers, compared to 32% of northern temperate and 29% of tropical species. Southern temperate transformer species are mainly woody trees and shrubs that were established on a grand scale as silvicultural crops, barriers (hedges, windbreaks and screens and cover/binders. Most aquatics, herbs, climbers and succulent shrubs an. trom the tropics. Ornamentals are the single largest category of plants from all three regions, the tropics having contributed twice as many species as temperate regions.

  3. Nymphon stipulum, a new pycnogonid species from Southern California

    NARCIS (Netherlands)

    Allan Child, C.

    1990-01-01

    A new species of pycnogonid, Nymphon stipulum, from off the Channel Islands, southern California, in 395 meters, is described and figured. Previous literature on southern California pycnogonids is outlined and the relationships of the new species with other southern California Nymphon species is dis

  4. New host range for Hematodinium in southern Australia and novel tools for sensitive detection of parasitic dinoflagellates.

    Science.gov (United States)

    Gornik, Sebastian G; Cranenburgh, Andrea; Waller, Ross F

    2013-01-01

    Hematodinium is a parasitic dinoflagellate and emerging pathogen of crustaceans. It preferably manifests in haemolymph of marine decapod crustaceans, killing a large variety of genera with significant impacts on fisheries worldwide. There is, however, evidence that some crustacean stocks harbor high prevalence, low intensity infections that may not result in widespread host mortality and are therefore hard to detect. The most widely used methods for detection of Hematodinium are conventional blood smears and polymerase chain reaction (PCR) against ribosomal RNAs. Blood smears demand a trained investigator, are labor intensive and not readily scalable for high-throughput sampling. PCRs only detect parasite DNA and can also suffer from false negatives and positives. In order to develop alternative detection tools for Hematodinium cells in decapod crustaceans we employed an immunological approach against a newly identified, abundant dinoflagellate-specific nuclear protein--Dinoflagellate/Viral NucleoProtein (DVNP). Both immunofluorescence assay (IFA) and Western blot methods against DVNP showed high sensitivity of detection. The Western blot detects Hematodinium parasites to levels of 25 parasites per milliliter of crustacean haemolymph, with the potential for sample pooling and screening of large samples. Using both PCR and these new tools, we have identified Hematodinium cells present in three new host crab taxa, at high prevalence but with no sign of pathogenesis. This extends the known range of Hematodinium to southern Australia.

  5. 地高辛标记的Northern blot检测鼠疫菌sRNA%Detection of Yersinia pestis sRNA by digoxigenin-labeled Northern blot

    Institute of Scientific and Technical Information of China (English)

    邓仲良; 苏山春; 孟祥荣; 吴移谋; 杨瑞馥; 韩延平

    2013-01-01

    [目的]随着高通量测序方法的应用,越来越多的sRNA (small non-coding RNA,sRNA)需验证.本研究建立用地高辛标记Northern blot检测鼠疫菌sRNA的技术,为细菌sRNA验证提供一种灵敏、特异的方法.[方法]在低铁条件下,提取鼠疫菌总RNA,10% dPAGE分离后电转到尼龙膜上并用紫外线交联RNA.膜经地高辛标记RyhB1或RyhB2寡核苷酸RNA探针过夜杂交后洗脱、封闭和免疫检测,最后曝光显影.[结果]地高辛标记的Northern blot曝光时间为20 s-3 min,RyhB1或RyhB2检测灵敏度分别为0.005 μg和0.05 μg.RyhB1或RyhB2探针特异性好,相互间无交叉反应.带正电或中性的尼龙膜都适用于杂交反应.RNA探针在42℃-65℃内杂交均可,提高温度可减少非特异性反应;而DNA探针杂交温度需摸索.[结论]本研究成功构建一种地高辛标记Northern blot检测鼠疫菌sRNA技术,具有特异性好、灵敏度高、探针易保存、曝光时间短等优点,为细菌sRNA验证和功能研究提供有利工具.%[Objective] With the application of high-throughput sequencing methods, more and more sRNAs are required to be verified.In this study, we developed the digoxigenin-labeled Northern blot method for detection of Yersinia pestis sRNA.[Methods] Total RNAs extracted from Yersinia.pestis grown under low-iron conditions were loaded onto 10% denaturing urea polyacrylamide gel (dPAGE) , electrophoresed and transferred to nylon membranes.Northern blots were fixed to the membrane by UV cross-linking and subjected to hybridization with 3 ' -end digoxigenin-labeled oligonucleotides RNA probe for RyhBl and RyhB2 overnight and followed by washing, blocking, immunological detection and finally exposed to film.[Results] Exposure time of digoxigenin-labelled Northern blot was 20s-3min.The detection sensitivity of RyhBl and RyhB2 was 0.005μg and 0.05 μg, respectively.RyhBl and RyhB2 probe specificity was high and no cross reaction with each other was found

  6. Comparison analysis between Western blot method and Magnetic particle Chemiluminescence method detection SLE autoantibody%免疫印迹法和磁微粒化学发光法检测SLE自身抗体的比对分析

    Institute of Scientific and Technical Information of China (English)

    周厚清

    2016-01-01

    目的:比较免疫印迹法(Western blot)和磁微粒化学发光法(Magnetic particle Chemiluminescence method)检测系统性红斑狼疮(SLE)自身抗体的一致性。方法:用免疫印迹法和磁微粒化学发光法对123份血清进行检测,其中系统性红斑狼疮阳性患者63例,健康体检者60例,用卡方检验对两种方法结果进行比较分析。结果:免疫印迹法和磁微粒化学发光法中抗Sm抗体阳性率分别为27.0%和14.3%、抗RNP/Sm阳性率分别为54.0%和42.9%、dsDNA阳性率分别为41.3%和30.2%、抗核小体抗体阳性率分别为31.7%和20.6%、抗核糖体P蛋白抗体阳性率分别为28.6%和11.1%。两种方法的准确性无显著性差异(>0.05)总符合率为80.6%,Kappa值为0.669。结论:免疫印迹法和磁微粒化学发光法检测系统性红斑狼疮自身抗体的检测结果具有可靠性,较好的符合率和准确性,磁微粒化学发光法可以作为简便、快速、经济的检测方法应用于系统性红斑狼疮自身抗体的检测。%Objective: The present study aims to compare Western blot and Magnetic particle Chemiluminescence method consistency on autoimmune antibody detection of systemic lupus erythematosus. Methods: Western blot assay and Magnetic particle Chemiluminescence method were used to detect autoimmune antibody from 123 serum samples, of which 63 patients with systemic lupus erythematosus, the others from 60 healthy persons. The results of the two methods were compared by X2 test. Results: Anti-Sm antibody positive rate of Western blot and Magnetic particle Chemiluminescence method were 27.0 and 14.3 percent respectively. Anti- RNP/Sm antibody positive rates were 39.7% and 42.9% respectively. Anti- ds- DNA antibody positive rates were 41.3% and 30.2% respectively. Anti- nucleosome antibody positive rates were 31.7% and 20.6% respectively. Anti- ribosomal protein antibody positive rates were 28.6% and 11.1% respectively. The accuracy

  7. Incidence of Phenylketonuria in Southern Iran

    Directory of Open Access Journals (Sweden)

    Asadollah Habib

    2010-06-01

    Full Text Available AbstractBackground: Phenylketonuria is a hereditary, autosomal recessivedisorder caused by deficiency of phenylalanine hydroxylaseor its cofactor tetrahydrobiopterin. The purpose ofthe present study was to evaluate the incidence of this disorderin southern Iran.Methods: All the neonates born between 22/Dec/2004 and7/Sep/2007 were screened and their blood samples were testedby colorimetric and high performance liquid chromatographymethods to obtain a diagnosis of phenylketonuria.Results: Of the screened newborns (87091 females and 88143males 15 female and 13 male neonates were diagnosed definitelyas having phenylketonuria.Conclusion: The incidence of phenylketonuria in girls andboys was 1.7 in 10000 and 1.5 in 10000, respectively (mean:1.6 in 10000 in southern Iran (Fars province.Iran J Med Sci 2010; 35(2: 137-139.

  8. Aedes albopictus breeding in southern Germany, 2014.

    Science.gov (United States)

    Werner, Doreen; Kampen, Helge

    2015-03-01

    Larvae, pupae and eggs of the Asian tiger mosquito Aedes albopictus were found in Freiburg, southern Germany, after submission of an adult mosquito specimen from that area to the 'Mückenatlas', a German instrument of passive mosquito surveillance. While previously collected Ae. albopictus in Germany were trapped on, or close to, service stations on motorways, suggesting introduction by vehicles from southern Europe, these new specimens were out of flight distance from the motorway on the one hand and indicate local reproduction on the other. The findings call for a thorough active and passive surveillance in exposed geographic regions such as the relatively warm German Upper Rhine Valley to prevent Ae. albopictus from establishing.

  9. Reemergence of Dengue in Southern Texas, 2013.

    Science.gov (United States)

    Thomas, Dana L; Santiago, Gilberto A; Abeyta, Roman; Hinojosa, Steven; Torres-Velasquez, Brenda; Adam, Jessica K; Evert, Nicole; Caraballo, Elba; Hunsperger, Elizabeth; Muñoz-Jordán, Jorge L; Smith, Brian; Banicki, Alison; Tomashek, Kay M; Gaul, Linda; Sharp, Tyler M

    2016-06-01

    During a dengue epidemic in northern Mexico, enhanced surveillance identified 53 laboratory-positive cases in southern Texas; 26 (49%) patients acquired the infection locally, and 29 (55%) were hospitalized. Of 83 patient specimens that were initially IgM negative according to ELISA performed at a commercial laboratory, 14 (17%) were dengue virus positive by real-time reverse transcription PCR performed at the Centers for Disease Control and Prevention. Dengue virus types 1 and 3 were identified, and molecular phylogenetic analysis demonstrated close identity with viruses that had recently circulated in Mexico and Central America. Of 51 household members of 22 dengue case-patients who participated in household investigations, 6 (12%) had been recently infected with a dengue virus and reported no recent travel, suggesting intrahousehold transmission. One household member reported having a recent illness consistent with dengue. This outbreak reinforces emergence of dengue in southern Texas, particularly when incidence is high in northern Mexico.

  10. Southern Pine Based on Biorefinery Center

    Energy Technology Data Exchange (ETDEWEB)

    Ragauskas, Arthur J. [Georgia Inst. of Technology, Atlanta, GA (United States); Singh, Preet [Georgia Inst. of Technology, Atlanta, GA (United States)

    2013-12-20

    This program seeks to develop an integrated southern pine wood to biofuels/biomaterials processing facility on the Recipient’s campus, that will test advanced integrated wood processing technologies at the laboratory scale, including: The generation of the bioethanol from pines residues and hemicelluloses extracted from pine woodchips; The conversion of extracted woodchips to linerboard and bleach grade pulps; and the efficient conversion of pine residues, bark and kraft cooking liquor into a useful pyrolysis oil.

  11. Southern Pine Based on Biorefinery Center

    Energy Technology Data Exchange (ETDEWEB)

    Ragauskas, Arthur J; Singh, Preet

    2014-01-10

    This program seeks to develop an integrated southern pine wood to biofuels/biomaterials processing facility on the Recipient’s campus, that will test advanced integrated wood processing technologies at the laboratory scale, including: • The generation of the bioethanol from pines residues and hemicelluloses extracted from pine woodchips; • The conversion of extracted woodchips to linerboard and bleach grade pulps; and • The efficient conversion of pine residues, bark and kraft cooking liquor into a useful pyrolysis oil.

  12. Intensity of plant collecting in southern Africa

    Directory of Open Access Journals (Sweden)

    G. E. Gibbs Russell

    1984-12-01

    Full Text Available The intensity of plant collecting in southern Africa is mapped using records from the Pretoria National Herbarium Computerized Information System (PRECIS, For the entire area, over 85% of the quarter degree grid squares have fewer than 100 specimens recorded. Collecting intensities are compared for different countries, biomes and climatic zones. Future field work from the National Herbarium will be concentrated in areas most seriously under-collected.

  13. Aspidonepsis (Asclepiadaceae, a new southern African genus

    Directory of Open Access Journals (Sweden)

    A. Nicholas

    1992-12-01

    Full Text Available Aspidonepsis, an endemic southern African genus, is described and compared to the closely allied genus Aspidoglossum. This newly described genus is composed of two subgenera, Aspidonepsis and Unguilobium. consisting of three and two species respectively.  Asclepias diploglossa, A. flava, A. cognata and A. reneensis are transferred to Aspidonepsis. and A. shebae is newly described. All species are discussed, illustrated and a key is given to aid in their identification.

  14. Broadband Seismic Studies In Southern Asia

    Science.gov (United States)

    2001-10-01

    the granulite terrain, the crust is both thicker (44 1 km deep) and more complicated, with a mid-crustal discontinuity at approximately 25-km depth...the granulite terrain (KOD, Fig. 1) the crust is 43 km thick. Thus, there are significant differences in Moho depth over southern India, but the...Moho there is about 5 km deeper, a result also noted in the Deep Seismic Soundings from the region. To the south of the shield in the granulite terrain

  15. Cholera outbreak--southern Sudan, 2007.

    Science.gov (United States)

    2009-04-10

    Vibrio cholerae causes cholera, an acute infectious diarrheal disease that can result in death without appropriate therapy, depending on the severity of the disease. War, poverty, inadequate sanitation, and large numbers of refugees and internally displaced persons (IDPs) are major precursors to cholera outbreaks. In 2005, Southern Sudan ended its 22-year civil war with North Sudan; as a result, IDPs and refugees are returning to the south. During April--June 2007, investigators from the Southern Sudan Field Epidemiology and Laboratory Training Program (SS-FELTP) and CDC investigated a cholera outbreak in the town of Juba, Southern Sudan. This report summarizes the results of that investigation, which found that 3,157 persons were diagnosed with suspected cholera during January--June 2007, with 74 deaths resulting from the disease. An environmental investigation revealed suboptimal hygiene practices and a lack of water and sanitation infrastructure in Juba. A case-control study indicated that persons less likely to have cholera were more likely to have consumed hot meals containing meat during the outbreak. Contaminated food or water were not identified as possible sources of the cholera outbreak in Juba. However, this might be attributed to limitations of the study, including small sample size. Cholera can reach epidemic proportions if adequate control measures are not implemented early. Mass media campaigns are important for current and new residents in Juba to understand the importance of proper food handling, clean water, and optimal hygiene practices to prevent the spread of cholera.

  16. Industrial Physics---Southern California Style

    Science.gov (United States)

    Leslie, Stuart

    2013-03-01

    Only in Southern California did space-age style really come into its own as a unique expression of Cold War scientific culture. The corporate campuses of General Atomic in San Diego and North American Aviation in Los Angeles perfectly expressed the exhilarating spirit of Southern California's aerospace era, scaling up the residential version of California modernism to industrial proportion. Architects William Pereira and A.C. Martin Jr., in collaboration with their scientific counterparts, fashioned military-industrial `dream factories' for industrial physics that embodied the secret side of the space-age zeitgeist, one the public could only glimpse of in photographs, advertisements, and carefully staged open houses. These laboratories served up archetypes of the California dream for a select audience of scientists, engineers, and military officers, live-action commercials for a lifestyle intended to lure the best and brightest to Southern California. Paradoxically, they hid in plain sight, in the midst of aerospace suburbs, an open secret, at once visible and opaque, the public face of an otherwise invisible empire. Now, at the end of the aerospace era, these places have become an endangered species, difficult to repurpose, on valuable if sometimes highly polluted land. Yet they offer an important reminder of a more confident time when many physicists set their sights on the stars.

  17. Geology of the Southern Appalachian Mountains

    Science.gov (United States)

    Clark, Sandra H.B.

    2008-01-01

    The Southern Appalachian Mountains includes the Blue Ridge province and parts of four other physiographic provinces. The Blue Ridge physiographic province is a high, mountainous area bounded by several named mountain ranges (including the Unaka Mountains and the Great Smoky Mountains) to the northwest, and the Blue Ridge Mountains to the southeast. Metamorphic rocks of the mountains include (1) fragments of a billion-year-old supercontinent, (2) thick sequences of sedimentary rock that were deposited in subsiding (sinking) basins on the continent, (3) sedimentary and volcanic rocks that were deposited on the sea floor, and (4) fragments of oceanic crust. Most of the rocks formed as sediments or volcanic rocks on ocean floors, islands, and continental plates; igneous rocks formed when crustal plates collided, beginning about 450 million years ago. The collision between the ancestral North American and African continental plates ended about 270 million years ago. Then, the continents began to be stretched, which caused fractures to open in places throughout the crust; these fractures were later filled with sediment. This product (U.S. Geological Survey Scientific Investigations Map 2830) consists of a geologic map of the Southern Appalachian Mountains overlain on a shaded-relief background. The map area includes parts of southern Virginia, eastern West Virginia and Tennessee, western North and South Carolina, northern Georgia and northeastern Alabama. Photographs of localities where geologic features of interest can be seen accompany the map. Diagrams show how the movement of continental plates over many millions of years affected the landscapes seen today, show how folds and faults form, describe important mineral resources of the region, and illustrate geologic time. This two-sided map is folded into a convenient size (5x9.4 inches) for use in the field. The target audience is high school to college earth science and geology teachers and students; staffs of

  18. Probabilistic Drought Forecasting in Southern Taiwan Using El Niño-Southern Oscillation Index

    Directory of Open Access Journals (Sweden)

    Shien-Tsung Chen

    2013-01-01

    Full Text Available This study proposes a probabilistic drought forecasting model to forecast meteorological drought in Southern Taiwan using the El Niño-SouthernNiño-Southern Oscillation (ENSO index. Meteorological drought is defined by the standardized precipitation index (SPI, and the ENSO index is El NiñoNiño sea surface temperature (SST. Two probabilistic forecasting model architectures were constructed based on the transition probabilities from El NiñoNiño SSTs to SPIs. Both model architectures forecast a one-month-ahead probability distribution for meteorological drought using different combinations of El NiñoNiño SST variables. Forecasting results showed the robustness of the probabilistic drought forecasting models. In addition, this study discussed the selection of El NiñoNiño SST variables used in the probabilistic drought forecasting model, and found that models with a single SST input outperformed those with multiple SST inputs.

  19. The impact of the Southern Annular Mode on future changes in Southern Hemisphere rainfall

    Science.gov (United States)

    Lim, Eun-Pa; Hendon, Harry H.; Arblaster, Julie M.; Delage, Francois; Nguyen, Hanh; Min, Seung-Ki; Wheeler, Matthew C.

    2016-07-01

    A robust positive trend in the Southern Annular Mode (SAM) is projected for the end of the 21st century under the Representative Concentration Pathway 8.5 scenario, which results in rainfall decreases in the midlatitudes and increases in the high latitudes in the Southern Hemisphere (SH). We find that this SAM trend also increases rainfall over the SH subtropics in austral summer but not in winter, leading to a pronounced wintertime poleward expansion of the subtropical dry zone. These dynamically driven rainfall changes by the SAM appear to oppose the thermodynamically driven projected rainfall changes in the SH subtropics and midlatitudes, whereas the two components reinforce each other in the high latitudes. However, we show that most climate models fall short in capturing the observed SAM component driven by the El Niño-Southern Oscillation and associated rainfall in the austral warm seasons, which limits our confidence in quantifying the contribution of the SAM to projected rainfall changes.

  20. Simultaneous identification of Aspergillus and Mucorales species by reverse line blot (RLB) hybridization assay%应用反向线点杂交技术鉴定临床常见曲霉属和毛霉目真菌

    Institute of Scientific and Technical Information of China (English)

    赵作涛; 李丽丽; 王晓阳; 万喆; 陈伟; 李若瑜

    2011-01-01

    Objective To Rapidly differentiate Aspergillus and Mucorales species by reverse line blot hybridization (RLB). Methods Totally 98 isolates including five Aspergillus strains ( Aspergillus fumigatus, Aspergillus flavus,Aspergillus niger, Aspergillus terreus and Aspergillus nidulans ) and seven Mucorales species ( Mucor heimalis ,Mucor racemosus ,Mucor cercinelloidea,Rhizopus arrhizus,Rhizopus microsporus,Rhizomucor pusillus and Absidia corymbifera ) were obtained from Research Center for Medical Mycology and Mycoses, Peking University. ITS1 and ITS4 fungal universal primers were chosen for PCR amplification, and the amplified products were used for reverse line blot hybridization with 12 fungal specie-specific probes. RLB data were compared with those by traditional fungal morphology and ITS sequencing methods. Results RLB showed high sensitivity and specificity , with 100% correct identification percentage of all the isolates and no cross hybridization between the species-specific probes. Eight negative control stains ( Candida albicans ,Fusarium solani ,Scedosporium apiospermum ,Penicillium marneffei ,Exophiala verrucosa,Aspergillus clavatus,Aspergillus japonicus and Cunninghamella elegans ) also showed negative by RLB. The analytical sensitivity of RLB was 1. 8 x 10-3 ng/μL by 10-fold serial dilution of Aspergillus fumigatus genomic DNA. Conclusions The RLB assay provides a rapid and reliable option for laboratory diagnosis and identification of Aspergillus and Mucorales species.%目的 应用反向线点杂交技术(reverse line blot hybridization,RLB)快速鉴定临床常见的曲霉属和毛霉目真菌.方法 收集我院真菌和真菌病研究中心保存的5种曲霉菌(烟曲霉、黄曲霉、黑曲霉、土曲霉、构巢曲霉)和7种毛霉目真菌(冻土毛霉菌、总状毛霉菌、卷枝毛霉菌、少根根霉、小孢根霉、微小根毛霉、伞状犁头霉),共计98株菌株.利用真菌通用引物ITS1和ITS4对菌株进行PCR扩增,用12个

  1. Comparison of linear array and line blot assay for detection of human papillomavirus and diagnosis of cervical precancer and cancer in the atypical squamous cell of undetermined significance and low-grade squamous intraepithelial lesion triage study.

    Science.gov (United States)

    Castle, Philip E; Gravitt, Patti E; Solomon, Diane; Wheeler, Cosette M; Schiffman, Mark

    2008-01-01

    We evaluated Linear Array (LA), a newly commercialized PGMY09/11 L1 consensus primer PCR test that detects 37 human papillomavirus (HPV) genotypes by reverse line blot hybridization, for the detection of individual HPV genotypes and carcinogenic HPV and its clinical performance for detecting 2-year cumulative cervical precancer and cancer using archived specimens from the Atypical Squamous Cell of Undetermined Significance (ASCUS) and Low-Grade Squamous Intraepithelial Lesion Triage Study. LA testing was conducted on enrollment specimens from women referred because of an ASCUS Pap test. To gauge the performance of the new test, the results were compared to those of its prototype predecessor assay, Line Blot Assay (LBA), restricted to paired results (n = 3,335). LA testing was done masked to LBA results and clinical outcomes. The results of LA and LBA testing were compared for detection of carcinogenic HPV and clinical outcomes of cervical precancer and cancer. Overall, 50% and 55% of the women tested positive for carcinogenic HPV by LBA and LA, respectively (P < 0.0001). The percent agreement for carcinogenic HPV detection was 88%, percent positive agreement was 80%, and kappa was 0.76 for detection of carcinogenic HPV by the two assays. There was a significant increase in detection by LA for most of the 37 HPV genotypes targeted by both assays, including for 13 of 14 carcinogenic HPV genotypes. LA detected more multiple-genotype infections for all HPV genotypes among HPV-positive women (P < 0.0001) and for carcinogenic HPV genotypes among carcinogenic-HPV-positive women (P < 0.0001). LA was more sensitive (92.3% versus 87.1%; P = 0.003) and less specific (48.2% versus 54.0%; P < 0.0001) than LBA for 2-year cumulative cervical precancer and cancer as diagnosed by the Pathology Quality Control Group. In conclusion, we found LA to be a promising assay for the detection of HPV genotypes and carcinogenic HPV, and it may be clinically useful for the detection of

  2. Comparison of Linear Array and Line Blot Assay for Detection of Human Papillomavirus and Diagnosis of Cervical Precancer and Cancer in the Atypical Squamous Cell of Undetermined Significance and Low-Grade Squamous Intraepithelial Lesion Triage Study▿

    Science.gov (United States)

    Castle, Philip E.; Gravitt, Patti E.; Solomon, Diane; Wheeler, Cosette M.; Schiffman, Mark

    2008-01-01

    We evaluated Linear Array (LA), a newly commercialized PGMY09/11 L1 consensus primer PCR test that detects 37 human papillomavirus (HPV) genotypes by reverse line blot hybridization, for the detection of individual HPV genotypes and carcinogenic HPV and its clinical performance for detecting 2-year cumulative cervical precancer and cancer using archived specimens from the Atypical Squamous Cell of Undetermined Significance (ASCUS) and Low-Grade Squamous Intraepithelial Lesion Triage Study. LA testing was conducted on enrollment specimens from women referred because of an ASCUS Pap test. To gauge the performance of the new test, the results were compared to those of its prototype predecessor assay, Line Blot Assay (LBA), restricted to paired results (n = 3,335). LA testing was done masked to LBA results and clinical outcomes. The results of LA and LBA testing were compared for detection of carcinogenic HPV and clinical outcomes of cervical precancer and cancer. Overall, 50% and 55% of the women tested positive for carcinogenic HPV by LBA and LA, respectively (P < 0.0001). The percent agreement for carcinogenic HPV detection was 88%, percent positive agreement was 80%, and kappa was 0.76 for detection of carcinogenic HPV by the two assays. There was a significant increase in detection by LA for most of the 37 HPV genotypes targeted by both assays, including for 13 of 14 carcinogenic HPV genotypes. LA detected more multiple-genotype infections for all HPV genotypes among HPV-positive women (P < 0.0001) and for carcinogenic HPV genotypes among carcinogenic-HPV-positive women (P < 0.0001). LA was more sensitive (92.3% versus 87.1%; P = 0.003) and less specific (48.2% versus 54.0%; P < 0.0001) than LBA for 2-year cumulative cervical precancer and cancer as diagnosed by the Pathology Quality Control Group. In conclusion, we found LA to be a promising assay for the detection of HPV genotypes and carcinogenic HPV, and it may be clinically useful for the detection of

  3. Taeniasis-cysticercosis in Southern Ecuador: assessment of infection status using multiple laboratory diagnostic tools

    Directory of Open Access Journals (Sweden)

    R Rodriguez-Hidalgo

    2006-11-01

    Full Text Available Taenia solium-taeniasis and cysticercosis were studied in the human and porcine populations of a rural community in the Southern Ecuadorian Andes. From the 1059 inhabitants, 800 serum samples and 958 stool samples could be collected. In addition, 646 from the estimated 1148 pigs were tongue inspected. Circulating antigen was detected by enzyme linked immunosorbent assay (Ag-ELISA in 2.25% of the human population, whereas intestinal taeniasis was detected in 1.46% by the formalin-ether technique. Following treatment and recovery of tapeworm fragments these were all identified as T. solium. Porcine cysticercosis was diagnosed in 3.56% of the pigs by tongue inspection. In addition, enzyme linked immunoelectrotransfer blot (EITB was performed on a subset group of 100 humans to confirm the results of the Ag-ELISA. One hundred serum samples from pigs were also analysed by EITB. It appeared that 43 and 74% of humans and pigs had antibodies against T. solium cysticerci, respectively. It is concluded that contrary to the high exposure of the human population to T. solium that is suggested by EITB, the number of active cysticercosis cases, diagnosed by Ag-ELISA, was low, which may indicate endemic stability. The further use of complementary diagnostic methods for a better understanding of the epidemiology of T. solium is suggested.

  4. Molecular epidemiology of extensively drug-resistant Klebsiella pneumoniae outbreak in Wenzhou, Southern China.

    Science.gov (United States)

    Du, Jia; Cao, Jianming; Shen, Lizhen; Bi, Wenzi; Zhang, Xiaoxiao; Liu, Haiyang; Lu, Hong; Zhou, Tieli

    2016-10-01

    The emergence of extensively drug-resistant (XDR) Klebsiella pneumoniae has become a major challenge worldwide. In this study, we characterized the phenotypes and genetic features of nine XDR K. pneumoniae isolates from an integrated hospital in Zhejiang province, China, from September to October 2014. These XDR K. pneumoniae possessed at least five resistance determinants which contribute to highly resistant to β-lactam, β-lactam/inhibitor combinations, aminoglycosides, quinolones, carbapenems, chloroamphenicol and fosfomycin. All isolates carried blaKPC-2, blaCTX-M-9, blaSHV-11 and rmtB, and several isolates also harboured blaTEM-1 and qnrS. Southern blot experiments confirmed that blaKPC-2, rmtB and blaCTX-M-9 were located on the same ~54.2 kb plasmid. Conjugative plasmids were obtained from all K. pneumoniae isolates, further proving the transferable characteristic of the resistance determinants. The OmpK36 sequences showed various deletions and insertions that indicated additional amino acid residues and a deleted phenotype of OmpK36. PFGE demonstrated that all the isolates belonged to the same genotype. Multilocus sequence typing was concordant with PFGE results and revealed that ST11 was the most predominant clone. Our study revealed a high incidence and endemic spread of XDR K. pneumoniae in the hospital. Thus, effective infection control measures should be adopted to monitor and control the spread of multidrug-resistant isolates.

  5. Taeniasis-cysticercosis in Southern Ecuador: assessment of infection status using multiple laboratory diagnostic tools.

    Science.gov (United States)

    Rodriguez-Hidalgo, R; Benitez-Ortiz, W; Praet, N; Saa, L R; Vercruysse, J; Brandt, J; Dorny, P

    2006-11-01

    Taenia solium-taeniasis and cysticercosis were studied in the human and porcine populations of a rural community in the Southern Ecuadorian Andes. From the 1059 inhabitants, 800 serum samples and 958 stool samples could be collected. In addition, 646 from the estimated 1148 pigs were tongue inspected. Circulating antigen was detected by enzyme linked immunosorbent assay (Ag-ELISA) in 2.25% of the human population, whereas intestinal taeniasis was detected in 1.46% by the formalin-ether technique. Following treatment and recovery of tapeworm fragments these were all identified as T. solium. Porcine cysticercosis was diagnosed in 3.56% of the pigs by tongue inspection. In addition, enzyme linked immunoelectrotransfer blot (EITB) was performed on a subset group of 100 humans to confirm the results of the Ag-ELISA. One hundred serum samples from pigs were also analysed by EITB. It appeared that 43 and 74% of humans and pigs had antibodies against T. solium cysticerci, respectively. It is concluded that contrary to the high exposure of the human population to T. solium that is suggested by EITB, the number of active cysticercosis cases, diagnosed by Ag-ELISA, was low, which may indicate endemic stability. The further use of complementary diagnostic methods for a better understanding of the epidemiology of T. solium is suggested.

  6. The Future of Southern Ocean Observing Systems

    Science.gov (United States)

    Talley, L. D.

    2015-12-01

    Knowledge of the Southern Ocean's role in global climate from seasonal to millennial timescales is evolving, with rapidly increasing recognition of the centrality of the Southern Ocean to Earth's heat, carbon, nutrient, and freshwater budgets, and of the impact of interactions between the ocean and the major ice shelves and grounded ice sheets of Antarctica, which have been decreasing in mass. Observations in this data-sparse and logistically remote region have never been so important, and many nations are rising to the challenge of supporting both experiments and long-term sustained observations. As illustrated in the figure from Meredith et al. (Current Op. Env. Sustain. 2013), autonomous in situ technologies are at the fore because of the difficulty and expense of sending ships year-round and because the crucial satellite remote sensing must be accompanied by in situ observations, including beneath sea ice and ice shelves. The Southern Ocean Observing System (SOOS) has grown out of this recognized need for coordinated observations from the Antarctic coastline northward to the subtropics, from the bottom water production regions in coastal polynyas over the continental shelves, to the regions of interaction of warm ocean waters with Antarctic ice shelves, beneath the vast seasonal sea ice region, and in the hot spots of air-sea fluxes and cross-Antarctic Circumpolar Current (ACC) mixing where the ACC interacts with topography and continental boundaries. The future includes international coordination and collaboration and strengthening of new and existing technologies, which include satellite observing, ice-enabled profiling floats, profiling from marine mammals, moored measurements in many strategic locations, glider and other autonomous operations in all regions, and drilling through floating ice shelves to measure the ocean waters below. Improved and consistent weather observations around the Antarctic coastlines will improve forecasting and reanalysis. Ice

  7. Late-glacial of southern South America

    Science.gov (United States)

    Heusser, C. J.

    Overall trends in late-glacial paleoenvironments of southern South America are interpretable from the pollen stratigraphy of radiocarbon dated sections of mires in Tierra del Fuego (55°S), the Chilotan archipelago (42-43°S), and the Chilean Lake District (39-41°S). In Tierra del Fuego, southern beech ( Nothofagus) and shrub and herb taxa (Gramineae, Empetrum, Acaena, Gunnera, Compositae and Cyperaceae) serve as indicators of the changing climate; in the Chilotan archipelago and in the Chilean Lake District, southern beech and other trees (species of Myrtaceae, Podocarpus, Prumnopitys, Pseudopanax and Weinmannia) suffice as indices of climatic change. Pollen records from each of these regions, although in need of greater dating control, indicate climatic sequences that are broadly similar. The records, however, are not regionally consistent in all aspects and differ in their indicator value with the implication of fossil beetle evidence. Attempts at correlation can be unsatisfactory at times and can stem inter alia from the different ecophysiological responses of both plants and beetles to environmental pressures. These differences, which affect the timing of reproduction and migration, may result in the variable occurrence of different species in the records. The broad implication of the pollen data is that following a glacial readvance culminating at about 15,000-14,500 BP, late-glacial climate was generally warmer during intervals before 13,000 and between 12,000 and 11,000 BP, and was cooler between 13,000 and 12,000 and from 11,000 to 10,000 BP.

  8. Epidemiology of cerebral palsy in Southern Denmark

    DEFF Research Database (Denmark)

    Frøslev-Friis, Christina; Dunkhase-Heinl, Ulrike; Andersen, Johnny Dohn Holmgren;

    2015-01-01

    INTRODUCTION: The aim of this study was to describe the prevalence, subtypes, severity and neuroimaging findings of cerebral palsy (CP) in a cohort of children born in Southern Denmark. Risk factors were analysed and aetiology considered. METHODS: A population-based cohort study covering 17...... prevention of CP is possible if the numbers of preterm births and multiple pregnancies can be reduced. FUNDING: The Danish Cerebral Palsy Follow-up Programme is supported by the foundation "Ludvig og Sara Elsass Fond". TRIAL REGISTRATION: 2008-58-0034....

  9. Northern and Southern RE Groups Ended Resultlessly

    Institute of Scientific and Technical Information of China (English)

    2005-01-01

    In September 2002, "Organizing of National Rare EarthEnterprises Group" which was delivered by original NationalEconomy & Trade Committee and original National PlanningCommittee, Ministry of Finance P.R.C, Ministry of Land &Resource P.R.C. and Ministry of Foreign Economy & Trade,was approved by the State Council of P.R.C. to organizeSouthern and Northern Rare Earth Groups. On October 30,2002, China Northern RE Group Co., Ltd Preparation Teamheld the foundation convention in Baotou, which drew thecurtain of ...

  10. Ceilometer measurements in the Southern Ocean

    Science.gov (United States)

    McDonald, Adrian; Alexander, Simon; French, John; Harvey, Mike; Ichoja, Andrew; Klekociuk, Andrew; Plank, Graeme; Katurji, Marwan

    2016-04-01

    Current climate models display a consistent deficit of reflected shortwave radiation over the Southern Ocean which is mainly due to the poor representation of clouds. Recent work has also shown that reanalysis also perform poorly relative to satellite observations in terms of cloud fraction. In particular, satellite observations have shown that low-level clouds (with tops below 3 km) are ubiquitous over the Southern Ocean. But, most satellite instruments, even the current generation of active satellite instruments, have difficulties in sampling low level clouds. As part of the New Zealand Deep South challenge project focussed on improving the representation of clouds in the Southern Ocean, we have begun to deploy autonomous instruments on 'ships of opportunity'. This study discusses measurements from a Väisälä CL51 laser ceilometer and ancillary instruments on the first two research voyages in the Southern Ocean and initial results. The route of the first voyage covers a return trip from Wellington (New Zealand) to Terra Nova Bay (Antarctica) onboard the R/V Tangaroa and occurred in January to mid-February 2015. The second deployment is onboard the Aurora Australis Australian Antarctic Division supply ship and began in October 2015 and is planned to finish at Macquarie Island in March 2016. The sampling provided by the ships route allows the ceilometer measurements of the height of the cloud base in a region where limited data apart from, potentially biased, satellite measurements of low-level cloud exist. Analysis of the boundary layer height derived from the ceilometer is also presented. The climatological structure derived from the ceilometer measurements is first detailed. We then compare these measurements with satellite and ground-based observations. We then examine variations in these measurements relative to their meteorological context. Details of plans for future voyages are also detailed. We will also present a preliminary analysis of a case study of

  11. The genus Waltheria in southern Africa

    Directory of Open Access Journals (Sweden)

    I. C. Verdoorn

    1981-12-01

    Full Text Available Waltheria indica L., the only species of Waltheria represented in southern Africa, is revised. This species, which occurs throughout the tropics and substropics of the world, is found abundantly in the northern Cape, Swaziland, northern Natal, Transvaal and northwards through South West Africa/Namibia and Botswana. Thoughout its wide distribution the species is uniform. A scrutiny o f herbarium specimens revealed that what appeared as a distinct species or subspecies was without doubt an abnormality, probably caused by insect injury.

  12. Taxonomy Icon Data: southern cassowary [Taxonomy Icon

    Lifescience Database Archive (English)

    Full Text Available southern cassowary Casuarius casuarius Chordata/Vertebrata/Aves Casuarius_casuarius_L.png Casuarius_cas...uarius_NL.png Casuarius_casuarius_S.png Casuarius_casuarius_NS.png http://biosciencedbc....jp/taxonomy_icon/icon.cgi?i=Casuarius+casuarius&t=L http://biosciencedbc.jp/taxonomy_icon/icon.cgi?i=Casuarius+cas...uarius&t=NL http://biosciencedbc.jp/taxonomy_icon/icon.cgi?i=Casuarius+casu...arius&t=S http://biosciencedbc.jp/taxonomy_icon/icon.cgi?i=Casuarius+casuarius&t=NS ...

  13. The Herpetology of the Southern Kalahari domain

    Directory of Open Access Journals (Sweden)

    W. D Haacke

    1984-12-01

    Full Text Available The herpetofauna of the southern Kalahari has mixed affinities, as this area lies on a rainfall gradient in a critical area where a transition between the arid south-west and the moister north-east takes place. As the variation in substrate type is relatively limited, the effect of the rainfall gradient appears to influence and determine the range limits of many taxa in both directions, resulting in an area in which of 55 recorded reptiles, 11 western taxa overlap or form a parapatric zone with 25 eastern taxa, while the remaining taxa are endemic or wideranging.

  14. Ethnobotanical remarks on Central and Southern Italy

    Directory of Open Access Journals (Sweden)

    Lucia Leporatti

    2007-05-01

    Full Text Available Abstract Background The present paper is a brief survey on the ethnobotanical works published by the Authors since 1981, concerning the research carried out in some southern and central Italian regions. Before Roman domination these territories were first inhabited by local people, while the southern areas were colonized by the Greeks. These different cultural contributions left certain traces, both in the toponyms and in the vernacular names of the plants and, more generally, in the culture as a whole. Methods Field data were collected through open interviews, mainly of farmers, shepherds and elderly people, born or living in these areas for a long time. Voucher specimens of collected plants are preserved in the respective herbaria of the Authors and in the herbarium of "Roma Tre" University. Important contributions have been made by several students native to the areas under consideration. A comparative analysis with local specific ethnobotanical literature was carried out. Results The paper reports several examples concerning human and veterinary popular medicine and in addition some anti-parasitic, nutraceutic, dye and miscellaneous uses are also described. Moreover vernacular names and toponyms are cited. Eight regions of central and southern Italy (particularly Latium, Abruzzo, Marche and Basilicata were investigated and the data obtained are presented in 32 papers. Most of the species of ethnobotanical interest have been listed in Latium (368 species, Marche (274 and Abruzzo (203. The paper also highlights particularly interesting aspects or uses not previously described in the specific ethnobotanical literature. Conclusion Phyto-therapy in central and southern Italy is nowadays practised by a few elderly people who resort to medicinal plants only for mild complaints (on the contrary food uses are still commonly practised. Nowadays therapeutic uses, unlike in the past, are less closely or not at all linked to ritual aspects. Several

  15. The Southern Tyrrhenian subduction system: recent evolution and neotectonic implications

    OpenAIRE

    A. Argnani

    2000-01-01

    Geological and geophysical data have been integrated with the aim of presenting a new evolutionary model for the Southern Tyrrhenian and adjacent regions. The Southern Tyrrhenian backarc basin opened within a plate convergence regime because of sinking and rollback of the oceanic Ionian lithosphere. On the basis of seismological observations, I infer that the sinking slab was torn apart on either side in the last 2 Ma and this process controlled the neotectonics of the Southern Apennines - Ty...

  16. Study on the Economic Differences between Northern and Southern Xinjiang

    Institute of Scientific and Technical Information of China (English)

    Juncheng; DONG

    2014-01-01

    There are great regional economic differences between northern and southern Xinjiang and the expansion of regional economic differences jeopardize social development and social stability,resulting in more serious polarization between the rich and the poor in northern and southern Xinjiang,thereby hampering further improvement of the economic efficiency in Xinjiang. Using factor analysis,this article carries out comprehensive analysis of regional economic differences in Xinjiang,derives the reasons for regional economic differences between northern and southern Xinjiang,and proposes recommendations for narrowing the regional economic differences between northern and southern Xinjiang.

  17. Anthropocene Survival of Southern New England's Salt ...

    Science.gov (United States)

    In southern New England, salt marshes are exceptionally vulnerable to the impacts of accelerated sea level rise. Regional rates of sea level rise have been as much as 50 % greater than the global average over past decades, a more than fourfold increase over late Holocene background values. In addition, coastal development blocks many potential marsh migration routes, and compensatory mechanisms relying on positive feedbacks between inundation and sediment deposition are insufficient to counter inundation increases in extreme low-turbidity tidal waters. Accordingly, multiple lines of evidence suggest that marsh submergence is occurring in southern New England. A combination of monitoring data, field re-surveys, radiometric dating, and analysis of peat composition have established that, beginning in the early and mid-twentieth century, the dominant low-marsh plant, Spartina alterniflora, has encroached upward in tidal marshes, and typical high-marsh plants, including Juncus gerardii and Spartina patens, have declined, providing strong evidence that vegetation changes are being driven, at least in part, by higher water levels. Additionally, aerial and satellite imagery show shoreline retreat, widening and headward extension of channels, and new and expanded interior depressions. Papers in this special section highlight changes in marsh-building processes, patterns of vegetation loss, and shifts in species composition. The final papers turn to strategies for minimiz

  18. Dermatoses in cement workers in southern Taiwan.

    Science.gov (United States)

    Guo, Y L; Wang, B J; Yeh, K C; Wang, J C; Kao, H H; Wang, M T; Shih, H C; Chen, C J

    1999-01-01

    Construction workers are known to have occupational dermatoses. The prevalence of such dermatoses was unknown in Taiwanese construction workers. The objective of this study was to determine the work exposure, prevalence of skin manifestations, and sensitivity to common contact allergens in cement workers of southern Taiwan. A total of 1147 current regular cement workers were telephone-interviewed about skin problems during the past 12 months, work exposure, and personal protection. Among those interviewed, 166 were examined and patch tested with common contact allergens. A high % of cement workers reported skin problems in the past 12 months. More men (13.9%) reported skin problems possibly related to work than women (5.4%). Prevalence was associated with lower use of gloves, duration of work as cement worker, and more time in jobs involving direct manual handling of cement, especially tiling. A high % of dermatitis was noted in the 166 workers examined, which correlated with reported skin problems. On patch testing, construction workers had a high frequency of sensitivity to chromate. Sensitivity to chromate or cobalt was associated with reported skin problems, or dorsal hand dermatitis on examination. These workers' dermatitis was under-diagnosed and inadequately managed. It is concluded that cement workers in southern Taiwan had a high prevalence of skin problems related to cement use. Protective measures, work practice, and physician education should be improved to prevent or manage such problems.

  19. Detector Considerations for a HAWC Southern Observatory

    CERN Document Server

    DuVernois, Michael

    2015-01-01

    The High-Altitude Water Cherenkov (HAWC) observatory in central Mexico is currently the world's only synoptic survey instrument for gamma rays above 1 TeV. Because there is significant interest in covering the full TeV sky with a survey instrument, we have examined options for a Southern Hemisphere extension to HAWC. In addition to providing all-sky coverage of TeV sources, a southern site could complement existing surveys of the densest part of the Galactic Plane, provide continuous monitoring of Galactic and extragalactic transient sources in both Hemispheres, and simplify the analysis of spatially extended signals such as diffuse gamma rays and the TeV cosmic-ray anisotropy. To take advantage of the air-shower physics and lower the energy threshold of the experiment as much as possible, a high altitude site above 5000 m a.s.l (vs. 4100 m a.s.l. at the current site in Mexico) has been specified. To facilitate efficient detector construction at such altitudes, the detector tanks would be assembled at lower a...

  20. Low frequency variability of Southern Ocean jets

    Science.gov (United States)

    Thompson, A. F.; Richards, K. J.

    2011-12-01

    Both observations and high resolution numerical models show that the Southern Ocean circumpolar flow is concentrated in a large number (approximately 8 to 12) of narrow filamentary jets. It is shown here that coherent jets exhibit a range of low frequency variability, on time scales of months to years, that can lead to displacement and to intermittent formation and dissipation of jets. Using output from an eddy-resolving ocean general circulation model in local regions near topographic features, the impact of energy exchange between eddy and mean flow components on jet persistence and variability is examined. A novel approach that uses a time-dependent definition of the mean flow provides a clearer picture of eddy-mean flow interactions in regions with spatially and temporally varying flow structure. The dynamics are largely consistent with those in idealized quasi-geostrophic models, including topographically-organized and surface-enhanced Reynolds stress forcing of the mean flow. Jets form during periods of enhanced eddy activity, but may persist long after the eddy activity has decayed. Similarly, jets may evolve in a downstream sense, with jet formation localized near topography and undergoing modification in response to changing bathymetry. The evolution of both temperature and potential vorticity is used to show that the low-frequency variability of the jets impacts water mass structure and tracer transport. This study highlights various examples of Southern Ocean dynamics that will prove difficult to capture through existing parameterizations in coarser climate models.

  1. Odonata (Insecta diversity of southern Gujarat, India

    Directory of Open Access Journals (Sweden)

    Darshana M. Rathod

    2016-09-01

    Full Text Available The diversity of the Odonata (dragonflies and damselflies was studied in seven districts of southern area of Gujarat State in India during 2014 to 2015.  A total of 55 species belonging to two suborders and 37 genera under eight families were recorded.  A total of 18 species of Zygoptera (damselflies and 37 species of Anisoptera (dragonflies were recorded.  Dang and Navsari districts were surveyed intensively and a maximum of 47 and 35 species were recorded respectively, whereas the districts that were surveyed less intensively, i.e., Bharuch (26, Valsad (21, Surat (29, Narmada (25 and Tapi (27 had comparatively low species richness.  Thirty-two species are being reported for the first time from southern Gujarat, raising the total list of odonates to 60.  Fifteen species namely, Lestes elatus Hagen in Selys, 1862; Elattoneura nigerrima (Laidlaw, 1917; Dysphaea ethela Fraser, 1924; Paracercion malayanum (Selys, 1876; Pseudagrion spencei Fraser, 1922; Burmagomphus laidlawi- Fraser, 1924; Cyclogomphus ypsilon Selys, 1854; Microgomphus torquatus (Selys, 1854; Onychogomphus acinaces (Laidlaw, 1922; Hylaeothemis indica Fraser, 1946; Lathrecista asiatica (Fabricius, 1798; Rhodothemis rufa (Rambur, 1842; Tramea limbata (Desjardins, 1832; Trithemis kirbyi Selys, 1891 and Zyxomma petiolatum Rambur, 1842 are recorded for the first time from Gujarat State raising the number of odonates of Gujarat State to 80 species.  

  2. Southern Criminology: Guest Editors’' Introduction

    Directory of Open Access Journals (Sweden)

    Russell Hogg

    2017-03-01

    Full Text Available Knowledge is a commodity and knowledge production does not occur in a geo-political vacuum. With respect to this, it has to be argued that neo-imperialism involves economic and knowledge flows across continuous space, which is transnational and distinct from the old forms of colonialism which were based on country-to-country occupation. In the context of contemporary geo-politics, these conditions render territorial terrain as less important than discursive terrain (Lo 2011. So, how is global knowledge in the social sciences (and more specifically in criminology produced and shared? Where does this production take place? Who are the producers? Whose experiences and whose voices are reflected in dominant academic discourses? How is knowledge disseminated and who gets access to it? These are some of the questions that the project of southern criminology seeks to tackle. To access the full text of the introductory article to this special issue on southern criminology, download the accompanying PDF file.

  3. The Use of Biotin to Demonstrate Immunohistochemistry, Western Blotting, and Dot Blots in University Practical Classes

    Science.gov (United States)

    Millar, Thomas James; Knighton, Ronald; Chuck, Jo-Anne

    2012-01-01

    Immunological detection of proteins is an essential method to demonstrate to undergraduate biology students, however, is often difficult in resource and time poor student laboratory sessions. This method describes a failsafe method to rapidly and economically demonstrate this technique using biotinylated proteins or biotin itself as targets for…

  4. 孕产妇HIV抗体筛查阳性与免疫印迹试验对比%Comparison of Positive of HIV Antibody Screening and Western Blot Test in Pregnant Women

    Institute of Scientific and Technical Information of China (English)

    朱厚宏; 王晨笛; 刘杨; 张祁; 孟建彤; 黄薇

    2011-01-01

    Objective To explore the relationship between the results of HIV antibody screening test and Westem Blot test ( WB) in pregnant women. Methods Thirty three positive samples from pregnant women were tested with the ELISA and Westem blot test (WB). Those with indefinite result by WB test were re-tested 3 and 6 months later, and HIV-1 viral load testing ( VL) was carried out at 6th month. The results of ELISA and the WB test were analyzed. Result The 33 samples were all positive by ELISA and 20 were HIV-1 positive by WB test. The ELISA and the WB test positive coincidence rate was 60. 61 % (20/33). Of 20 samples 0were with S/CO value more than 8, the WB test results of HIV-1 antibody were all positive. Of 13 samples were with S/CO value less than 1 , 5 were negative and 8 were indefinite in WB test. The HIV-1 viral load test result at 6th month was less than the minimum detection limit (50 IU/ml ) . Conclusion The false positive in HIV antibody screening test (ELISA) was observed. The false positive sample were in the WB test negative and indefinite ones. ELISA and the WB test positive coincidence rate increased with the S/CO value of ELISA. The HIV WB test indefinite result in pregnant women was mostly due to non-specific immune response.%目的 探讨孕产妇人类免疫缺陷病毒(HIV)抗体筛查试验阳性与免疫印迹试验(WB)结果的关系.方法 对33份孕产妇HIV抗体待复查样本,进行酶联免疫吸附试验(ELISA )及免疫印迹试验(WB),对WB试验结果不确定者进行3个月、6个月随访检测,6个月随访样本补充HIV-1病毒载量检测,分析ELISA检测与WB试验检测结果.结果 33份样本ELISA复检均呈阳性,免疫印迹试验(WB)中20份确认为HIV-1抗体阳性,ELISA检测与WB试验阳性符合率为60.61%(20/33); S/CO值>8的20份样本,WB试验结果均为HIV-1抗体阳性,I < S/CO值<8的13份样本,WB试验5份为HIV抗体阴性,8份为HIV抗体不确定;6个月随访样本HIV-1病毒载量检测,

  5. 亚洲带绦虫囊尾蚴特异性抗原筛选和鉴定%Screen and detection of specific antigens of Taenia asiatica cysticercus with two dimensional electrophoresis, western blotting and MS/MS spectrometry

    Institute of Scientific and Technical Information of China (English)

    方文; 肖靓靓; 包怀恩; 牟荣

    2011-01-01

    Objective To screen and identify specific antigens of Taenia asiatica cysticercus and to predict the function of target proteins using bioinformatics method. Methods Six three-way cross hybrid pigs were randomly divided into experimental group and control group and each experimental pig was infected with 84 000 eggs. Serum samples were collected 40 days after the infection. The total protein of Taenia asiatica cysticercus was separated by two dimensional electrophoresis and western blotting was performed to find out distinct antigens. Proteins from the two groups were identified by electrospray ion trap mass spectrometry(ESI-Trap MS). Results Totally 204 ± 11 spots were detected through Coomassie brilliant blue stained gels with relative molecular weight( Mr) of 14 400 -94 000 kDa and isoelectric point(pl) of 3. 0 - 10. 0. Western-blotting showed 6 specific antigen spots with pool sera of infected pigs. Two of the 6 antigens with known functions were ascribed to ATPase family gene 3-like protein 2 and ATP synthase beta subunit. Conclusion Two specific antigens of Taenia asiatica cysticercus have been identified.%目的 对亚洲带绦虫囊尾蚴的特异性抗原进行筛选、鉴定和生物信息学分析.方法 6头20d龄三元杂交乳猪随机分为实验组和对照组,实验组每头灌胃亚洲带绦虫虫卵悬液8.4万个;20 d后,分别制备实验组及对照组心脏血混合血清;收集、制备实验组亚洲带绦虫囊尾蚴蛋白并进行双向电泳分析,将凝胶蛋白斑点转移至聚偏氟乙烯膜,用实验组及对照组混合血清(均为1∶10)为一抗,羊抗猪辣根过氧化物酶- IgG(1∶200)为二抗,进行蛋白质印迹分析和质谱鉴定.结果 双向电泳凝胶共检测到( 204±11)个蛋白质斑点,相对分子质量为14 400 ~94 000 kPa,等电点(pI)为3.0~10.0;筛选出6个特异性抗原,经质谱鉴定出其中2个蛋白,分别为ATP酶管家基因3-类蛋白2(AT-Pase family gene 3-like protein 2)

  6. Comparison of multiplex real-time PCR and PCR-reverse blot hybridization assay for the direct and rapid detection of bacteria and antibiotic resistance determinants in positive culture bottles.

    Science.gov (United States)

    Wang, Hye-Young; Kim, Seoyong; Kim, Jungho; Park, Soon Deok; Kim, Hyo Youl; Uh, Young; Lee, Hyeyoung

    2016-09-01

    The aim of this study was to evaluate the performance of a commercially available multiplex real-time PCR assay and a PCR-reverse blot hybridization assay (PCR-REBA) for the rapid detection of bacteria and identification of antibiotic resistance genes directly from blood culture bottles and to compare the results of these molecular assays with conventional culture methods. The molecular diagnostic methods were used to evaluate 593 blood culture bottles from patients with bloodstream infections. The detection positivity of multiplex real-time PCR assay for Gram-positive bacteria, Gram-negative bacteria and Candida spp. was equivalent to PCR-REBA as 99.6 %, 99.1 % and 100 %, respectively. Using conventional bacterial cultures as the gold standard, the sensitivity, specificity, positive predictive value and negative predictive value of these two molecular methods were 99.5 % [95 % confidence interval (CI), 0.980-1.000; PReal-methicillin-resistant Staphylococcusaureus multiplex real-time PCR assay targeting the mecA gene to detect methicillin resistance was lower than that of the PCR-REBA method, detecting an overall positivity of 98.4 % (n=182; 95 % CI, 0.964-1.000; P<0.009) and 99.5 % (n=184; 95 % CI, 0.985-1.000; P<0.0001), respectively. The entire two methods take about 3 h, while results from culture can take up to 48-72 h. Therefore, the use of these two molecular methods was rapid and reliable for the characterization of causative pathogens in bloodstream infections.

  7. Use of the Falcon assay screening test--enzyme-linked immunosorbent assay (FAST-ELISA) and the enzyme-linked immunoelectrotransfer blot (EITB) to determine the prevalence of human fascioliasis in the Bolivian Altiplano.

    Science.gov (United States)

    Hillyer, G V; Soler de Galanes, M; Rodriguez-Perez, J; Bjorland, J; Silva de Lagrava, M; Ramirez Guzman, S; Bryan, R T

    1992-05-01

    A collaborative study between the University of Puerto Rico School of Medicine, the Centers for Disease Control, the Bolivian Ministry of Health, and private voluntary organizations (Foster Parents Plan International and Danchurchaid) working in Bolivia has identified a region in the northwestern Altiplano of Bolivia near Lake Titicaca as harboring the highest prevalence of human fascioliasis in the world reported to date. Two serologic techniques (the Falcon assay screening test-enzyme-linked immunosorbent assay [FAST-ELISA] and the enzyme-linked immunoelectrotransfer blot [EITB]) were used in the determination of its prevalence. One hundred serum samples and 73 stool samples were obtained from Aymara Indians from Corapata, Bolivia. Antibody absorbance levels to Fasciola hepatica excretion-secretion antigens were compared with EITB banding patterns using the same antigen preparation. A positive FAST-ELISA result was defined as an absorbance value greater than the mean plus three standard deviations of two sets of normal negative controls (Puerto Rican and Bolivian). Using this criterion, 53 of 100 sera tested were found positive by this technique. Within this group, 19 (95%) of 20 individuals who were parasite positive were also positive by FAST-ELISA. An additional 24 individuals who were negative for F. hepatica eggs and 10 individuals for whom no specimens were received were also positive by FAST-ELISA. Among the 53 individuals negative for F. hepatica eggs, 29 were also negative by FAST-ELISA. The EITB analysis of the sera from confirmed infected individuals revealed at least three F. hepatica (Fh) bands with molecular weights of 12, 17, and 63 kD, respectively.(ABSTRACT TRUNCATED AT 250 WORDS)

  8. A comparison of real-time PCR and reverse line blot hybridization in detecting feline haemoplasmas of domestic cats and an analysis of risk factors associated with haemoplasma infections

    Directory of Open Access Journals (Sweden)

    Georges Karla

    2012-07-01

    Full Text Available Abstract Background Three species of feline haemoplasma are recognised: Mycoplasma haemofelis (Mhf, ‘Candidatus Mycoplasma haemominutum’ (CMhm and ‘Candidatus Mycoplasma turicensis (CMt. This study compared a reverse line blot hybridization (RLB assay for simultaneous detection of Mhf, CMhm with three separate quantitative real-time polymerase chain reaction (qPCR assays used for diagnosis of Mhf, CMhm and CMt. The RLB and qPCR assays were applied to DNA extracted from blood samples collected from 154 cats from Trinidad and Tobago. Results CMhm and Mhf DNA were detected using both RLB and qPCR. CMt DNA was detected by qPCR only. Comparing RLB and qPCR for the detection of CMhm DNA, 40 (26.3% and 48 (31.6% cats, respectively, were positive. The difference was more marked for Mhf, with RLB detecting a total of only 11 (7.2% positive cats whereas qPCR detected 41 (27.0% positive cats. Using qPCR as a gold standard, haemoplasma infected cats were more likely to be retrovirus positive (OR = 5.68, P = 0.02 and older (median age 5.5 years, than non-infected cats. In addition, CMhm positive cats were more likely to be male (OR = 3.4, P = 0.04. Conclusions Overall the qPCR was more sensitive than RLB. In addition, age (median 5.5 years and retrovirus positivity were risk factors for infection with the feline haemoplasmas in this study population. Further studies on feline haemoplasma infections in cats are needed to determine the significance of detecting small amounts of haemoplasma DNA, feline retrovirus infection and other associated risk factors on the clinical manifestation of disease.

  9. Monitoring expression profiles of rice genes under cold, drought, and high-salinity stresses and abscisic acid application using cDNA microarray and RNA gel-blot analyses.

    Science.gov (United States)

    Rabbani, M Ashiq; Maruyama, Kyonoshin; Abe, Hiroshi; Khan, M Ayub; Katsura, Koji; Ito, Yusuke; Yoshiwara, Kyoko; Seki, Motoaki; Shinozaki, Kazuo; Yamaguchi-Shinozaki, Kazuko

    2003-12-01

    To identify cold-, drought-, high-salinity-, and/or abscisic acid (ABA)-inducible genes in rice (Oryza sativa), we prepared a rice cDNA microarray including about 1700 independent cDNAs derived from cDNA libraries prepared from drought-, cold-, and high-salinity-treated rice plants. We confirmed stress-inducible expression of the candidate genes selected by microarray analysis using RNA gel-blot analysis and finally identified a total of 73 genes as stress inducible including 58 novel unreported genes in rice. Among them, 36, 62, 57, and 43 genes were induced by cold, drought, high salinity, and ABA, respectively. We observed a strong association in the expression of stress-responsive genes and found 15 genes that responded to all four treatments. Venn diagram analysis revealed greater cross talk between signaling pathways for drought, ABA, and high-salinity stresses than between signaling pathways for cold and ABA stresses or cold and high-salinity stresses in rice. The rice genome database search enabled us not only to identify possible known cis-acting elements in the promoter regions of several stress-inducible genes but also to expect the existence of novel cis-acting elements involved in stress-responsive gene expression in rice stress-inducible promoters. Comparative analysis of Arabidopsis and rice showed that among the 73 stress-inducible rice genes, 51 already have been reported in Arabidopsis with similar function or gene name. Transcriptome analysis revealed novel stress-inducible genes, suggesting some differences between Arabidopsis and rice in their response to stress.

  10. Dynamics of the southern California current system

    Science.gov (United States)

    di Lorenzo, Emanuele

    The dynamics of seasonal to long-term variability of the Southern California Current System (SCCS) is studied using a four dimensional space-time analysis of the 52 year (1949--2000) California Cooperative Oceanic Fisheries Investigations (CalCOFI) hydrography combined with a sensitivity analysis of an eddy permitting primitive equation ocean model under various forcing scenarios. The dynamics of the seasonal cycle in the SCCS can be summarized as follows. In spring upwelling favorable winds force an upward tilt of the isopycnals along the coast (equatorward flow). Quasi-linear Rossby waves are excited by the ocean adjustment to the isopycnal displacement. In summer as these waves propagate offshore poleward flow develops at the coast and the Southern California Eddy (SCE) reaches its seasonal maxima. Positive wind stress curl in the Southern California Bight is important in maintaining poleward flow and locally reinforcing the SCE with an additional upward displacement of isopycnals through Ekman pumping. At the end of summer and throughout the fall instability processes within the SCE are a generating mechanism for mesoscale eddies, which fully develop in the offshore waters during winter. On decadal timescales a warming trend in temperature (1 C) and a deepening trend in the depth of the mean thermocline (20 m) between 1950 and 1998 are found to be primarily forced by large-scale decadal fluctuations in surface heat fluxes combined with horizontal advection by the mean currents. After 1998 the surface heat fluxes suggest the beginning of a period of cooling, which is consistent with colder observed ocean temperatures. The temporal and spatial distribution of the warming is coherent over the entire northeast Pacific Ocean. Salinity changes are decoupled from temperature and uncorrelated with indices of large-scale oceanic variability. Temporal modulation of southward horizontal advection by the California Current is the primary mechanism controlling local

  11. Seismic unrest at Katla Volcano- southern Iceland

    Science.gov (United States)

    jeddi, zeinab; Tryggvason, Ari; Gudmundsson, Olafur; Bödvarsson, Reynir; SIL Seismology Group

    2014-05-01

    Katla volcano is located on the propagating Eastern Volcanic Zone (EVZ) in South Iceland. It is located beneath Mýrdalsjökull ice-cap which covers an area of almost 600 km2, comprising the summit caldera and the eruption vents. 20 eruptions between 930 and 1918 with intervals of 13-95 years are documented at Katla which is one of the most active subglacial volcanoes in Iceland. Eruptions at Katla are mainly explosive due to the subglacial mode of extrusion and produce high eruption columns and catastrophic melt water floods (jökulhlaups). The present long Volcanic repose (almost 96 years) at Katla, the general unrest since 1955, and the 2010 eruption of the neighbouring Eyjafjallajökull volcano has prompted concerns among geoscientists about an imminent eruption. Thus, the volcano has been densely monitored by seismologists and volcanologists. The seismology group of Uppsala University as a partner in the Volcano Anatomy (VA) project in collaboration with the University of Iceland and the Icelandic Meteorological Office (IMO) installed 9 temporary seismic stations on and around the Mýrdalsjökull glacier in 2011. Another 10 permanent seismic stations are operated by IMO around Katla. The project's data collection is now finished and temporary stations were pulled down in August 2013. According to seismicity maps of the whole recording period, thousands of microearthquakes have occurred within the caldera region. At least three different source areas are active in Katla: the caldera region, the western Godaland region and a small cluster at the southern rim of Mýrdalsjökull near the glacial stream of Hafursarjökull. Seismicity in the southern flank has basically started after June 2011. The caldera events are mainly volcano-tectonic, while western and southern events are mostly long period (lp) and can be related to glacial or magmatic movement. One motivation of the VA Katla project is to better understand the physical mechanism of these lp events. Changes

  12. Evaluation of network RTK in southern Ontario

    Science.gov (United States)

    Saeidi, Amir

    Network Real-Time Kinematic (RTK) has become popular in the past decade as an efficient method of precise, real-time positioning. Its relatively low cost and ease-of-use makes it a good candidate to replace static relative Global Positioning System (GPS) in, e.g., land surveying. A lack of previous studies aroused the interest of the Ministry of Transportation of Ontario (MTO) to request York University to complete a comprehensive study of the performance of network RTK in southern Ontario and whether it is a suitable method for MTO control surveying. Extensive fieldwork campaigns in the winter of 2010 and summer of 2011 were carried out and ˜300 hours of static and ˜50 hours of kinematic network RTK data were collected from three different service providers. A set of metrics were defined to characterize the performance of network RTK: availability, time-to-first-fix, precision, accuracy, solution integrity and moving average filtering. The data were used to characterize the horizontal performance of network RTK services and the results along with a set of guidelines and specifications were provided (Saeidi et al., 2011; Bisnath et al., 2012). This thesis presents the horizontal network RTK performance evaluation, as well as the vertical and kinematic performance. The aforementioned metrics are used to evaluate the quality of network RTK in southern Ontario, and to compare to similar services available in other locations. The result have revealed expected ˜2-3 cm (95%) precision for the horizontal and vertical components; however, large horizontal and vertical biases were observed, which can be as high as 4 cm. The solution integrity has shown that typically, 3σ solution uncertainties are larger than the actual errors, unless large biases exist. Moving average filtering has confirmed that due to large outliers and spikes in the solutions, 1 second observation periods are not sufficient to provide a precise solution; larger observation windows should be used, e

  13. 33 CFR 100.1101 - Southern California annual marine events.

    Science.gov (United States)

    2010-07-01

    ... 33 Navigation and Navigable Waters 1 2010-07-01 2010-07-01 false Southern California annual marine events. 100.1101 Section 100.1101 Navigation and Navigable Waters COAST GUARD, DEPARTMENT OF HOMELAND SECURITY REGATTAS AND MARINE PARADES SAFETY OF LIFE ON NAVIGABLE WATERS § 100.1101 Southern California annual marine events. (a) Special...

  14. Evolution of HIV/AIDS in Southern Africa

    NARCIS (Netherlands)

    Lauwers, L.; Pruyt, E.; Hens, L.; Brans, J.p.

    2008-01-01

    This paper presents a system dynamics model to study the spread of HIV/AIDS in Southern Africa. The HIV/AIDS model includes important feedback mechanisms of the spread of HIV/AIDS, and partly explains the dynamics of the epidemic in a representative Southern African country. The HIV/AIDS model indic

  15. Exploration Breakthrough in Southern Margin of Junggar Basin

    Institute of Scientific and Technical Information of China (English)

    Kang Zhulin; Zheng Jiandong

    1997-01-01

    @@ The Hutubi structure, located in the southern margin of Junggar Basin, is 60 km norrthwest of Urumqi and 20 km northwest of Changji (Fig. l). The Hu-2 well drilled in the Hutubi structure has gained high-yield gas flow,significant exploration breakthrough in the southern margin of Junggar Basin since the 1950s.

  16. The earliest unequivocally modern humans in southern China

    NARCIS (Netherlands)

    Liu, Wu; Martinón-Torres, María; Cai, Yan-Jun; Xing, Song; Tong, Hao-Wen; Pei, Shu-Wen; Sier, Mark Jan; Wu, Xiao-Hong; Edwards, R Lawrence; Cheng, Hai; Li, Yi-Yuan; Yang, Xiong-Xin; de Castro, José María Bermúdez; Wu, Xiu-Jie

    2015-01-01

    The hominin record from southern Asia for the early Late Pleistocene epoch is scarce. Well-dated and well-preserved fossils older than ∼45,000 years that can be unequivocally attributed to Homo sapiens are lacking. Here we present evidence from the newly excavated Fuyan Cave in Daoxian (southern Chi

  17. Biogeography and ecology of southern Portuguese butterflies and burnets (Lepidoptera)

    NARCIS (Netherlands)

    Schmitt, T.

    2003-01-01

    Biogeography and ecology of southern Portuguese butterflies and burnets (Lepidoptera) During several visits to the western part of the Algarve (southern Portugal), the author mapped the butterflies and burnets of this region. In total, I observed 58 butterfly species (51 Papilionoidea, 7 Hesperiidae

  18. Plutonium in Southern Hemisphere ocean Waters

    DEFF Research Database (Denmark)

    Hirose, K.; Aoyama, M.; Gastaud, J.

    2013-01-01

    Plutonium in seawater collected by the BEAGLE2003 cruise was determined using ICP- SF-MS and alpha spectrometry after Fe co-precipitation and radiochemical purification. Levels and distributions of dissolved plutonium activity concentrations in Southern Hemisphere ocean waters are summarized here......, including historical data. Pu-239 concentrations in surface water----of the central South Pacific (32.5 °S) in 2003 were around 1 mBq/m3. The 239Pu concentrations in the Indian Ocean surface waters (20°S) were similar to that in the South Pacific, whereas the 239Pu concentrations in the South Atlantic...... surface waters (30°S) were markedly lower than those in the South Pacific and Indian Oceans. The 239Pu vertical profile pattern was similar to that in the North Pacific subtropical gyre, although 239Pu concentrations in the deep South Pacific were significantly lower than those in the North Pacific. One...

  19. The Southern Cone Initiative against Chagas disease.

    Science.gov (United States)

    Schofield, C J; Dias, J C

    1999-01-01

    Chagas disease (also known as American trypanosomiasis) is now ranked as the most serious parasitic disease of the Americas, with an economic impact far outranking the combined effects of other parasitic diseases such as malaria, schistosomiasis and leishmaniasis. Although the chronic infection remains virtually incurable, transmission can be halted by eliminating the domestic insect vectors and screening blood donors to avoid transfusional transmission. In line with this strategy, governments of the six Southern Cone countries (Argentina, Bolivia, Brazil, Chile, Paraguay and Uruguay) launched in 1991 an ambitious initiative to control Chagas disease through elimination of the main vector, Triatoma infestans, and large-scale screening of blood donors. Now at its mid-point, the programme has achieved remarkable success, with transmission halted over vast areas of the previously endemic regions. Well over 2 million rural houses have been sprayed to eliminate T. infestans, and the programme has already shown significant economic rates of return in addition to the medical and social benefits.

  20. Solvent Extraction Developments in Southern Africa

    Institute of Scientific and Technical Information of China (English)

    2006-01-01

    The largest solvent-extraction plant in the world at the time, the Nchanga Copper Operation, was in Zambia. The first commercial process using solvent extraction for the refining of the platinum-group metals was in South Africa. More recently, the Southern African region has seen the implementation of solvent extraction for other base metals, precious metals, and specialty metals. These include the world firsts of primary production of zinc at Skorpion Zinc in Namibia and the large-scale refining of gold by Harmony Gold in South Africa. Several other flowsheets that use solvent-extraction technology are currently under commissioning, development, or feasibility study for implementation in this part of the world, including those for the recovery of copper, cobalt, nickel, tantalum, and niobium.

  1. Southern marl prairies conceptual ecological model

    Science.gov (United States)

    Davis, S.M.; Loftus, W.F.; Gaiser, E.E.; Huffman, A.E.

    2005-01-01

    About 190,000 ha of higher-elevation marl prairies flank either side of Shark River Slough in the southern Everglades. Water levels typically drop below the ground surface each year in this landscape. Consequently, peat soil accretion is inhibited, and substrates consist either of calcitic marl produced by algal periphyton mats or exposed limestone bedrock. The southern marl prairies support complex mosaics of wet prairie, sawgrass sawgrass (Cladium jamaicense), tree islands, and tropical hammock communities and a high diversity of plant species. However, relatively short hydroperiods and annual dry downs provide stressful conditions for aquatic fauna, affecting survival in the dry season when surface water is absent. Here, we present a conceptual ecological model developed for this landscape through scientific concensus, use of empirical data, and modeling. The two major societal drivers affecting the southern marl prairies are water management practices and agricultural and urban development. These drivers lead to five groups of ecosystem stressors: loss of spatial extent and connectivity, shortened hydroperiod and increased drought severity, extended hydroperiod and drying pattern reversals, introduction and spread of non-native trees, and introduction and spread of non-native fishes. Major ecological attributes include periphyton mats, plant species diversity and community mosaic, Cape Sable seaside sparrow (Ammodramus maritimus mirabilis), marsh fishes and associated aquatic fauna prey base, American alligator (Alligator mississippiensis), and wading bird early dry season foraging. Water management and development are hypothesized to have a negative effect on the ecological attributes of the southern marl prairies in the following ways. Periphyton mats have decreased in cover in areas where hydroperiod has been significantly reduced and changed in community composition due to inverse responses to increased nutrient availability. Plant species diversity and

  2. [Toxicity and apple production in southern Brazil].

    Science.gov (United States)

    Klanovicz, Jó

    2010-03-01

    The article explores the links between the controversial apprehension of contaminated apples in southern Brazil in 1989 and the reactions of the apple industry to press reports on the use of pesticides in Brazilian orchards. The issue is framed within a broader analysis of the notions of toxicity and 'danger' surrounding the consumption of healthier food and the idea of 'food security,' notions that have begun taking hold in public and private life. It is argued that apple growers' responses to the problem can be better understood through a historical reading of the interactions between the biology of the apple tree, the agroecology of this monoculture, and the structures, actors, and discourses of the human and non-human groups in Brazil's apple-producing region.

  3. Thickness diffusivity in the Southern Ocean

    Science.gov (United States)

    Eden, Carsten

    2006-06-01

    Thickness diffusivity ($\\kappa$) according to the Gent and McWilliams parameterisation which accounts for eddy-driven advection in the ocean, is estimated using output from an eddy-resolving model of the Southern Ocean. A physically meaningful definition of rotational eddy fluxes leads almost everywhere to positive $\\kappa$. Zonally averaged near surface values of $\\kappa$ remain smaller than 200 m2/s poleward of the polar front, increases between 60-45°S to about 600 m2/s and peak between 45-35° S at almost 3000 m2/s. $\\kappa$ stays high in the upper 500 m but decreases with depth and is essentially zero below 2500 m. In addition to the thickness diffusion ($\\kappa$) there is eddy-induced eastward (westward) advection of isopycnal thickness at the poleward (equatorward) flank of the ACC pointing toward strong anisotropic lateral mixing.

  4. Brazil to Join the European Southern Observatory

    Science.gov (United States)

    2010-12-01

    The Federative Republic of Brazil has yesterday signed the formal accession agreement paving the way for it to become a Member State of the European Southern Observatory (ESO). Following government ratification Brazil will become the fifteenth Member State and the first from outside Europe. On 29 December 2010, at a ceremony in Brasilia, the Brazilian Minister of Science and Technology, Sergio Machado Rezende and the ESO Director General, Tim de Zeeuw signed the formal accession agreement aiming to make Brazil a Member State of the European Southern Observatory. Brazil will become the fifteen Member State and the first from outside Europe. Since the agreement means accession to an international convention, the agreement must now be submitted to the Brazilian Parliament for ratification [1]. The signing of the agreement followed the unanimous approval by the ESO Council during an extraordinary meeting on 21 December 2010. "Joining ESO will give new impetus to the development of science, technology and innovation in Brazil as part of the considerable efforts our government is making to keep the country advancing in these strategic areas," says Rezende. The European Southern Observatory has a long history of successful involvement with South America, ever since Chile was selected as the best site for its observatories in 1963. Until now, however, no non-European country has joined ESO as a Member State. "The membership of Brazil will give the vibrant Brazilian astronomical community full access to the most productive observatory in the world and open up opportunities for Brazilian high-tech industry to contribute to the European Extremely Large Telescope project. It will also bring new resources and skills to the organisation at the right time for them to make a major contribution to this exciting project," adds ESO Director General, Tim de Zeeuw. The European Extremely Large Telescope (E-ELT) telescope design phase was recently completed and a major review was

  5. Prevalence of Asthma in Southern Punjab, Pakistan.

    Directory of Open Access Journals (Sweden)

    Maryum Khan

    2016-01-01

    Full Text Available AbstractAsthma is exaggerated response of immune system which is a leading cause of death in the third world. Main causes of asthma are allergy, smoking, drugs like NSAID (Aspirin and family history. Objective of study was to check the prevalence of asthma in different age groups and its impact on socioeconomical behaviors of the peoples of southern Punjab, by developing a questionnaire. Incidence of asthmatic attack in the age group of 20 to 60 years was more than in age group of 20 years, furthermore the incidence was found to be more common in females as compare to males. The smokers were at more risk to develop the disease as compared to the nonsmokers.

  6. The Southern Argentine Agile Meteor Radar (SAAMER)

    Science.gov (United States)

    Janches, Diego

    2014-11-01

    The Southern Argentina Agile Meteor Radar (SAAMER) is a new generation system deployed in Rio Grande, Tierra del Fuego, Argentina (53 S) in May 2008. SAAMER transmits 10 times more power than regular meteor radars, and uses a newly developed transmitting array, which focuses power upward instead of the traditional single-antenna-all-sky configuration. The system is configured such that the transmitter array can also be utilized as a receiver. The new design greatly increases the sensitivity of the radar enabling the detection of large number of particles at low zenith angles. The more concentrated transmitted power enables additional meteor studies besides those typical of these systems based on the detection of specular reflections, such as routine detections of head echoes and non-specular trails, previously only possible with High Power and Large Aperture radars. In August 2010, SAAMER was upgraded to a system capable to determine meteoroid orbital parameters. This was achieved by adding two remote receiving stations approximately 10 km away from the main site in near perpendicular directions. The upgrade significantly expands the science that is achieved with this new radar enabling us to study the orbital properties of the interplanetary dust environment. Because of the unique geographical location, SAAMER allows for additional inter-hemispheric comparison with measurements from Canadian Meteor Orbit Radar, which is geographically conjugate. Initial surveys show, for example, that SAAMER observes a very strong contribution of the South Toroidal Sporadic meteor source, of which limited observational data is available. In addition, SAAMER offers similar unique capabilities for meteor showers and streams studies given the range of ecliptic latitudes that the system enables detailed study of showers at high southern latitudes (e.g July Phoenicids or Puppids complex). Finally, SAAMER is ideal for the deployment of complementary instrumentation in both, permanent

  7. SRAO: the first southern robotic AO system

    Science.gov (United States)

    Law, Nicholas M.; Ziegler, Carl; Tokovinin, Andrei

    2016-08-01

    We present plans for SRAO, the first Southern Robotic AO system. SRAO will use AO-assisted speckle imaging and Robo-AO-heritage high efficiency observing to confirm and characterize thousands of planet candidates produced by major new transit surveys like TESS, and is the first AO system to be capable of building a comprehensive several-thousand-target multiplicity survey at sub-AU scales across the main sequence. We will also describe results from Robo-AO, the first robotic LGS-AO system. Robo-AO has observed tens of thousands of Northern targets, often using a similar speckle or Lucky-Imaging assisted mode. SRAO will be a moderate-order natural-guide-star adaptive optics system which uses an innovative photoncounting wavefront sensor and EMCCD speckle-imaging camera to guide on faint stars with the 4.1m SOAR telescope. The system will produce diffraction-limited imaging in the NIR on targets as faint as mν = 16. In AO-assisted speckle imaging mode the system will attain the 30-mas visible diffraction limit on targets at least as faint as mν = 17. The system will be the first Southern hemisphere robotic adaptive optics system, with overheads an order of magnitude smaller than comparable systems. Using Robo-AO's proven robotic AO software, SRAO will be capable of observing overheads on sub-minute scales, allowing the observation of at least 200 targets per night. SRAO will attain three times the angular resolution of the Palomar Robo-AO system in the visible.

  8. Online Geophysical Databases for the Southern Ocean

    Science.gov (United States)

    Goodwillie, A.; O'Hara, S.; Arko, R.; Carbotte, S.; Ryan, W.; Melkonian, A.; Ferrini, V.; Weissel, R.; Bonczkowski, J.

    2007-12-01

    With funding from the U.S. National Science Foundation Office of Polar Programs, the Antarctic Multibeam Bathymetry Synthesis (AMBS, http://www.marine-geo.org/antarctic/) is an integrated web-accessible bathymetry and geophysical database for the Southern Ocean and Antarctica, serving data from the US research vessels Nathaniel B. Palmer and Laurence M. Gould, amongst others. Interdisciplinary polar data can be downloaded for free through the Data Link web browser interface (http://www.marine-geo.org/link/) which enables keyword searches by data and instrument type, geographical bounds, scientist, expedition name and dates. The free, platform-independent data visualization tool GeoMapApp (http://www.geomapapp.org/) supports dynamic exploration of a wide range of data sets on a Global Multi-Resolution Topography (GMRT) synthesis, including the polar regions, allowing users to generate custom grids and maps and import their own data sets and grids. A specialised polar stereographic map projection incorporating multibeam swath bathymetry and the BEDMAP under-ice seafloor topography is available for the Southern Ocean. The GMRT global digital elevation model is served freely as a Web Map Service layer and is available for viewing with OGC-compliant clients including Google Earth (http://www.marine-geo.org/Data4GoogleEarth.html). To promote interoperability and data sharing, we are working with research partners including the Marine Metadata Interoperability (MMI) project and the National Geophysical Data Center to develop standardised metadata and best practices that comply with existing FGDC and ISO standards. We are also taking on the US Antarctic Data Coordination Center function, assisting NSF-funded investigators in documenting and archiving their data in accordance with the IPY Data Policy.

  9. Observations of seismic activity in Southern Lebanon

    Science.gov (United States)

    Meirova, T.; Hofstetter, R.

    2013-04-01

    Recent seismic activity in southern Lebanon is of particular interest since the tectonic framework of this region is poorly understood. In addition, seismicity in this region is very infrequent compared with the Roum fault to the east, which is seismically active. Between early 2008 and the end of 2010, intense seismic activity occurred in the area. This was manifested by several swarm-like sequences and continuous trickling seismicity over many days, amounting in total to more than 900 earthquakes in the magnitude range of 0.5 ≤ M d ≤ 5.2. The region of activity extended in a 40-km long zone mainly in a N-S direction and was located about 10 km west of the Roum fault. The largest earthquake, with a duration magnitude of M d = 5.2, occurred on February 15, 2008, and was located at 33.327° N, 35.406° E at a depth of 3 km. The mean-horizontal peak ground acceleration observed at two nearby accelerometers exceeded 0.05 g, where the strongest peak horizontal acceleration was 55 cm/s2 at about 20 km SE of the epicenter. Application of the HypoDD algorithm yielded a pronounced N-S zone, parallel to the Roum fault, which was not known to be seismically active. Focal mechanism, based on full waveform inversion and the directivity effect of the strongest earthquake, suggests left-lateral strike-slip NNW-SSE faulting that crosses the NE-SW traverse faults in southern Lebanon.

  10. Probabilistic Drought Forecasting in Southern Taiwan Using El Niño-Southern Oscillation Index

    Directory of Open Access Journals (Sweden)

    Shien-Tsung Chen

    2013-01-01

    Full Text Available This study proposes a probabilistic drought forecasting model to forecast meteorological drought in Southern Taiwan using the El Niño-Southern Oscillation (ENSO index. Meteorological drought is defined by the standardized precipitation index (SPI, and the ENSO index is El Niño sea surface temperature (SST. Two probabilistic forecasting model architectures were constructed based on the transition probabilities from El Niño SSTs to SPIs. Both model architectures forecast a one-month-ahead probability distribution for meteorological drought using different combinations of El Niño SST variables. Forecasting results showed the robustness of the probabilistic drought forecasting models. In addition, this study discussed the selection of El Niño SST variables used in the probabilistic drought forecasting model, and found that models with a single SST input outperformed those with multiple SST inputs.

  11. Syntectonic remagnetization in the southern Methow block: Resolving large displacements in the southern Canadian Cordillera

    Science.gov (United States)

    Enkin, R.J.; Mahoney, J.B.; Baker, J.; Kiessling, M.; Haugerud, R.A.

    2002-01-01

    The Upper Cretaceous Ventura Member of the Goat Wall unit in the southern Methow block of southern British Columbia and northern Washington State holds a syntectonic magnetization. Eight new sites from Manning Park in British Columbia give a mean direction of D = 27.5??, I = 60.1??, k = 304.7, ??95 = 3.2?? after optimal partial tilt correction. Of five groups of bedded sites from farther south in the basin reported by Bazard et al. [1990], four have a syntectonic remanence with a direction similar to what we observe. The exception is one group which has optimal concentration of remanence directions on >100% untilting and an abherent direction which must be rejected. Combining the accepted sites, the optimal differential syntilting direction is D = 11.8??, I = 61.5??, k = 39.3, ??95 = 3.4?? (N = 47), giving a mean pole of 79.8??N, 359.2??E, K = 19.5, and A95 = 4.8??. The age of the remagnetization is constrained to be between 88 and 80 Ma. Compared to cratonic North America, this result indicates that the southern Methow block was displaced from the south by 1800 ?? 500 km, meaning it lay south of the Sierra Nevada subduction zone but well north of other paleomagnetically constrained Cretaceous rock units from the Insular superterrane, including correlative strata of the Mount Tatlow area in the northern Methow block. Among several possibilities to reconcile this discrepancy, the most plausible has the whole Methow block translated coherently but with the southern Methow block strata remagnetized during transit.

  12. Reconstruction of Early Pleistocene climate in southern Australia, and implications for Southern Hemisphere atmospheric circulation

    Science.gov (United States)

    Sniderman, K.; Porch, N.

    2009-12-01

    Southern Australia today experiences a winter-dominated rainfall regime, governed by the seasonal migration of the Southern Hemisphere subtropical anticyclone. The history of this rainfall seasonality regime is poorly understood, because well dated, late Neogene climate records are scarce, both in Australia and in the Southern Hemisphere more generally. However, it has been assumed that the initiation of this rainfall regime was linked to the onset of extensive Northern Hemisphere glaciation, around 2.6 Ma. Here, we use fossil beetle remains from an upland southeastern Australian palaeolake record to quantitatively reconstruct regional climate during ~280 kyr of the Early Pleistocene, between 1.84-1.56 Ma. Our results, based on coexistence of extant fossil beetle taxa, indicate that temperatures were consistently 1-3°C warmer than present, and annual rainfall as high as or higher than today, but summer rainfall was 2-2.4 times higher than today, throughout the period of our record. This is consistent with the presence in the associated pollen record of diverse rainforest plant taxa, many of which are now extinct regionally or Australia-wide. Our results indicate that the modern, winter-dominated rainfall regime was not yet in place by 1.5 Ma, at least one million years later than previously thought. In as much as the modern climate regime is a function of the intensity of atmospheric circulation driven by hemispheric temperature gradients, this implies that Southern Hemisphere anticyclonic circulation may have been much less intense than today during the Early Pleistocene, and that a major regional climate reorganisation (Ravelo et al., 2004) occurred sometime after 1.5 Ma, perhaps associated with further growth of the Antarctic cryosphere (Raymo et al., 2006).

  13. Musci austro-africani II. Bryophyte collections in southern Africa and southern African type specimens in the National Herbarium, Pretoria

    Directory of Open Access Journals (Sweden)

    R. E. Magill

    1980-12-01

    Full Text Available A brief review of bryological collections and collectors in southern Africa introduces a catalogue of southern African type specimens housed in the National Herbarium, Pretoria. The type catalogue, arranged alphabetically by basionym, includes correct names, type status and label data.

  14. Northerners versus southerners: Italian anthropology and psychology faced with the "southern question".

    Science.gov (United States)

    Cimino, Guido; Foschi, Renato

    2014-11-01

    Following the Unification of Italy (1861), when confronted with the underdevelopment problems of the south that had given rise to the so-called "southern question," some Italian anthropologists and psychologists began to study the populations of the south from the psycho-anthropological point of view. These scientists, at times subject to preconceived ideas toward the southerners, conveyed observations and descriptions of the southern character traits that, in general, were considered different, in a negative sense, with respect to those of the northern peoples. To explain such diversity in the "psychological" characteristics between the north and south of the country (presumed cause also of the south's backwardness), various hypotheses were advanced related to the kind of heredity theory adopted, which could be of, more or less, an "innatist" or "transformist" or "environmentalist" kind. The distinction proposed in this article between at least 2 different "hereditarian" theories formulated by the Italian scientists, and the confrontation of these theories with the hypotheses expressed by the "southernist" sociologists, contrary to the idea of "racial varieties" present in the Italian population, allows one to understand in what way and in what sense, at the threshold of the 20th century, there arose the ideology of "Nordicism" and the roots of racism were planted.

  15. Ecology and control of an introduced population of Southern Watersnakes (Nerodia fasciata) in southern California

    Science.gov (United States)

    Reed, Robert; Todd, Brian D; Miano, Oliver J.; Canfield, Mark; Fisher, Robert N.; McMartin, Louanne

    2016-01-01

    Native to the southeastern United States, Southern Watersnakes (Nerodia fasciata) are known from two sites in California, but their ecological impacts are poorly understood. We investigated the ecology of Southern Watersnakes in Machado Lake, Harbor City, Los Angeles County, California, including an assessment of control opportunities. We captured 306 watersnakes as a result of aquatic trapping and hand captures. We captured snakes of all sizes (162–1063 mm snout–vent length [SVL], 3.5–873.3 g), demonstrating the existence of a well-established population. The smallest reproductive female was 490 mm SVL and females contained 12–46 postovulatory embryos (mean  =  21). Small watersnakes largely consumed introduced Western Mosquitofish (Gambusia affinis), while larger snakes specialized on larval and metamorph American Bullfrogs (Lithobates catesbeianus) and Green Sunfish (Lepomis cyanellus). Overall capture per unit effort (CPUE) in traps declined with time during an intensive 76-d trapping bout, but CPUE trends varied considerably among traplines and it is unlikely that the overall decline in CPUE represented a major decrease in the snake population size. Although we found no direct evidence that Southern Watersnakes are affecting native species in Machado Lake, this population may serve as a source for intentional or unintentional transportation of watersnakes to bodies of water containing imperiled native prey species or potential competitors.

  16. 实时PCR和PCR-RDB法检测人乳头瘤病毒的比对研究%Comparison of real time PCR and PCR¯reverse dot blot hybridization for detection of Human papillomavirus

    Institute of Scientific and Technical Information of China (English)

    肖克林; 严泽浩; 罗茗月; 麦光兴; 陈熙; 熊礼宽

    2014-01-01

    目的:比较实时 PCR 法和 PCR-反向点杂交法(PCR-RDB)检测 HPV 的一致性。方法收集109份女性生殖道样本,利用实时 PCR 法和 PCR-RDB 法分别检测 HPV 感染和基因型分布情况,不一致样本采用 PCR-悬浮芯片杂交法复检。结果83.5%(91/109)的样本两种方法结果一致(kappa=0.671),18例不一致样本 PCR-悬浮芯片杂交法复检显示7例与实时 PCR相符,11例与 PCR-RDB 相符;高、低病毒载量组间 PCR-RDB 法的检测结果差异无统计学意义(χ2=1.476,P =0.224)。结论实时 PCR 和 PCR-RDB 两法用于 HPV 检测一致性一般;HPV 病毒载量在103~108范围内时 PCR-RDB 法的阳性率较稳定。%Objective To compare real time PCR with PCR-reverse dot blot hybridization (PCR-RDB)for detecting human pap-illomavirus (HPV)infection in women.Methods A total of 109 genital specimens from women were collected in the study.All specimens were tested HPV by using real time PCR and PCR-RDB,discrepant samples were tested again by PCR-xMAP.Results The concordant rate was 83.5%(91/109)between real time PCR and PCR-RDB (kappa=0.671),the other 18 discrepant samples were retested by PCR-xMAP,7 of those were identical with real time PCR and 11 with PCR-RDB.No differences of PCR-RDB pos-itive rates were found between the high and low viral load groups (χ2 =1.476,P =0.224).Conclusion It demonstrated moderate consistency between real time PCR and PCR-RDB.The HPV positive rates of PCR-RDB were stable when the viral loads were 103-108 .

  17. Serological Thymidine Kinase 1 is a Biomarker for Early Detection of Tumours—A Health Screening Study on 35,365 People, Using a Sensitive Chemiluminescent Dot Blot Assay

    Directory of Open Access Journals (Sweden)

    Ellen He

    2011-11-01

    Full Text Available Serological thymidine kinase 1 (STK1 is a reliable proliferation marker for prognosis, monitoring tumour therapy, and relapse. Here we investigated the use of STK1 in health screening for early detection of pre-malignant and malignant diseases. The investigation was based on 35,365 participants in four independent health screening studies in China between 2005–2011. All participants were clinically examined. The concentration of STK1 was determined by a sensitive chemiluminescent dot blot ECL assay. The ROCvalue of the STK1 assay was 0.96. At a cut-off STK1 value of 2.0 pM, the likelihood (+ value was 236.5, and the sensitivity and the specificity were 0.78 and 0.99, respectively. The relative number of city-dwelling people with elevated STK1 values (≥2.0 pM was 0.8% (198/26,484, while the corresponding value for the group of oil-field workers was 5.8% (514/8,355. The latter group expressed significantly higher frequency of refractory anaemia, fatty liver, and obesity, compared to the city dwellers, but no cases of breast hyperplasia or prostate hyperplasia. Furthermore, people working in oil drilling/oil transportation showed higher STK1 values and higher frequency of pre-malignancies and benign diseases than people working in the oil-field administration. In the STK1 elevated group of the city-dwelling people, a statistically significantly higher number of people were found to have malignancies, pre-malignancies of all types, moderate/severe type of hyperplasia of breast or prostate, or refractory anaemia, or to be at high risk for hepatitis B, compared to people with normal STK1 values (< 2.0 pM. No malignancies were found in the normal STK1 group. In the elevated STK1 group 85.4% showed diseases linked to a higher risk for pre-/early cancerous progression, compared to 52.4% of those with normal STK1 values. Among participants with elevated STK1 values, 8.8% developed new malignancies or progress in their pre-malignancies within 5 to 72

  18. 单抗免疫斑点法和组织印迹法检测侵染蝴蝶兰的建兰花叶病毒%Detection of CymMV in Orchids Tissue by Dot-ELISA and Tissue Blot-ELISA with Monoclonal Antibody

    Institute of Scientific and Technical Information of China (English)

    董晓辉; 孟春梅; 黎军英; 吴建祥; 荣松; 张超; 洪健

    2009-01-01

    Dot-ELISA and tissue blot-ELISA with monoclonal antibody to detect Cymbidium mosaic virus (CymMV) were established. 8000-dilution CymMV monoclonal antibody could be adopted to detect CymMV infecting leaf extract within a dilution limitation of 1:10240. CymMV could still be detected in the eighth print by tissue blot-ELISA . Similar results were shown between the fifth print by tissue blot-ELISA and 80-dilution of infected leaf extract by dot-ELISA. Comparison tests showed that the commonly used tissue blot-ELISA was less sensitive than dot-ELISA. It could illustrate that CymMV monoclonal antibody was more specific and sensitive than the polyclonal antibodies. The improved tissue blot-ELISA is more simple than dot-ELISA, and is suitable for fast detection with a large number of samples in the field.%应用抗建兰花叶病毒(CymMV)的单克隆抗体,建立了快速检测蝴蝶兰病样的免疫斑点法(Dot-ELISA)和组织印迹法(Tissue blot-ELISA).CymMV单抗稀释8000倍时,Dot-ELISA可检出病毒粗汁液的最大稀释度为1:10240;Tissue blot-ELISA中样品1次平切后1~5次印迹与Dot-ELISA样品1:80稀释结果相当,6~8次印迹与Dot-ELISA 1:320稀释结果相当,前8次印迹均可以得到满意的检测效果.Tissue blot-ELISA的灵敏度略低于Dot-ELISA,使用单抗的结果比多抗特异性强,操作更为简便,适合大量兰花样品的快速检测.

  19. Potential Dust Emissions from Sources in the Southern Hemisphere.

    Science.gov (United States)

    Bhattachan, A.; D'Odorico, P.; Okin, G. S.

    2014-12-01

    The Southern Hemisphere currently exhibits low levels of atmospheric dust concentrations relative to the Northern Hemisphere. Recent research suggests that dust concentrations could, however, increase as a result of loss of vegetation cover in the Southern Kalahari and the Mallee. Disturbances resulting from grazing and agriculture are identified as such drivers of land use change in these regions. While studies on the importance of atmospheric dust in global-scale processes are abundant, little has been done to locate the potential dust sources in the Southern Hemisphere because potential new sources are by definition inactive and are undetected in satellite images. To this end, using a combination of laboratory experiments and field observations, we assess that the sediments collected from the dunefields in the Southern Kalahari and Mallee can emit substantial amount of dust, are rich in soluble iron and dust from these sources would reach the Southern Ocean. It is suggested that the supply of soluble iron through atmospheric dust deposition limits the productivity of the Southern Ocean. Thus intensification of land use can potentially make these regions an important source of iron given their proximity to the Southern Ocean. This iron-rich dust could stimulate ocean productivity in future as more areas are reactivated as a result of land-use and droughts.

  20. Processes controlling Southern Ocean cloud-climate feedbacks (Invited)

    Science.gov (United States)

    Kay, J. E.; Medeiros, B.; Hwang, Y.; Gettelman, A.

    2013-12-01

    We use a fully coupled climate model (CESM) to identify processes controlling intriguingly diverse Southern Ocean cloud feedbacks in response to increased greenhouse gas forcing. Modeled Southern Ocean cloud-climate feedbacks range from the most positive (enhancing greenhouse warming at ~40 degrees South) to the most negative (damping greenhouse warming at ~60 degrees South) on the planet. As greenhouse gas concentrations increase, Antarctic sea ice loss, warming, and a poleward stormtrack shift/sub-tropical expansion all modify Southern Ocean clouds. Our analysis shows that Southern Ocean clouds are controlled both by thermodynamics (cloud changes for a given subsidence rate) and by dynamics (changes in subsidence rates). Hinting at the importance of thermodynamics, absorbed shortwave radiation over the Southern Ocean is substantially more affected by increased greenhouse gas forcing than by a poleward stormtrack shift in the absence of greenhouse forcing. While we find CESM a useful tool, CESM has substantial Southern Ocean biases (e.g., excessive Antarctic sea ice, excessive absorbed shortwave radiation). Thus, we also assess the impact that these biases have on the realism of CESM Southern Ocean cloud-climate greenhouse feedbacks.

  1. Stars and Nebulae in the Southern Crown

    Science.gov (United States)

    2000-10-01

    The R Coronae Australis complex of young stars and interstellar gas clouds is one of the nearest star-forming regions, at a distance of approx. 500 light-years from the Sun. It is seen in the southern constellation of that name (The "Southern Crown"). Images of this sky area were recently obtained with the Wide Field Imager (WFI) , a 67-million pixel digital camera that is installed at the 2.2-m MPG/ESO Telescope at ESO's La Silla Observatory. Some of these exposures have been combined into a magnificent colour image, here reproduced as PR Photo 25a/00 . The field shown measures about 4.7 x 4.7 light-years 2. It displays the central part of the complex, its brightest stars, and the nebulosity that they illuminate. The interstellar clouds that are associated with the complex are visible all across this field and also beyond its borders (on other exposures), due to the obscuring effect of the dust particles that "dim" the light of stars behind these clouds. This effect is particularly noticeable in the lower left corner where very few stars are seen. R Coronae Australis , the bright star from which the entire complex is named, is located at the center of the field and illuminates the reddish nebula around it. The bright star in the lower part, illuminating a somewhat bluer nebula, is known as TY Coronae Australis . The brightness of these two stars and several others in the same field is variable. They belong to the so-called "T Tauri" class , a type that is quite common in star-forming regions. T Tauri stars are in the early stages of stellar evolution and display various observable characteristics of this phase, e.g. emission at visible and infrared wavelengths due to the accretion of matter left over from their formation, as well as X-ray emission. The nebulosity seen in this picture is mostly due to reflection of the stellar light by small dust particles. The stars in the R Coronae Australis complex do not emit sufficient ultraviolet light to ionize a substantial

  2. Stars and Seasons in Southern Africa

    Science.gov (United States)

    Snedegar, K. V.

    Although the indigenous people of Southern Africa traditionally viewed the sky as a place quite apart from the Earth, they believed celestial phenomena to be natural signs united with those of the Earth in a harmonious synchronicity. There is no substantial evidence that the precolonial Africans imagined a casual relationship between celestial bodies and the seasonal patterns of life on Earth. They did, however, recognize a coincidental relationship. The traditional African cosmos, then, worked as a noetic principle unifying the observed motions of celestial bodies, the sequence of seasons, and the behavior of plants and animals. Such a cosmos, with local peculiarities, was widely understood in Southern Africa before the end of the last century. By the early 20th century European colonial paradigms had largely obliterated this African worldview. This paper will offer a partial reconstruction. Pre-colonial South African people viewed time as a sequence of discrete natural events; through annual repetition these events served as a guide for proper human action. The South Africans analyzed the passage of time in terms of the motions of celestial bodies, the maturation of beneficial plants, and the mating patterns of animals. The rightful course of human life was seen to fit within the seasonal context of these natural phenomena. The visibility of conspicuous stars and asterisms marked significant times of year. For instance, the Lovedu people greeted the dawn rising of Canopus with joy: "The boy has come out." The star was a signal for rainmaking and boys' initiation ceremonies to proceed. The Venda constellation Thutlwa, the giraffes, comprises α and β Crucis and α and β Centauri. In October Thutlwa skims the trees of the evening horizon. The Venda Thutlwa literally means 'rising above the trees,' an allusion to the majestic vegetarian creatures and the stars advising the people to be done with their spring planting. This paper will describe stellar associations

  3. Olivine in the Southern Isidis Basin

    Science.gov (United States)

    2007-01-01

    The Compact Reconnaissance Imaging Spectrometer for Mars (CRISM) took this observation of the transition region between Libya Montes and the Isidis Basin on Mars at 17:16 UTC (12:16 p.m. EST) on January 2, 2007, near 3.6 degrees north latitude, 84.1 degrees east longitude. The image was taken in 544 colors covering 0.36-3.92 micrometers, and shows features as small as 18 meters (60 feet) across. The image is about 11 kilometers (7 miles) wide at its narrowest point. The Isidis Basin resulted from of a gigantic impact on the surface of Mars early in the planet's history. The southern rim, where this target is located, is a region of complex geology and part of the planetary dichotomy boundary that separates the older southern highlands from the lower, younger northern plains. The image on the left was constructed from three visible wavelengths (RGB: 0.71, 0.60, 0.53 microns) and is a close approximation of how the surface would appear to the human eye. The image on the right was constructed from three infrared wavelengths (RGB: 2.49, 1.52, 1.08 microns) chosen to highlight variations in the mineralogy of the area. Of interest is that features in this image not only differ in color, but also in texture and morphology. The gray areas absorb similarly at all wavelengths used in this image, but display absorptions at other wavelengths related to the iron- and magesium-rich mineral pyroxene. The reddest areas absorb strongly at the wavelengths used for green and blue, which is attributable to another iron- and magesium-rich mineral, olivine. The brownish areas show subdued mineral absorptions and could represent some type of mixture between the other two materials. The presence of the mineral olivine is particularly interesting because olivine easily weathers to other minerals; thus, its presence indicates either the lack of weathering in this region or relatively recent exposure. CRISM's mission: Find the spectral fingerprints of aqueous and hydrothermal deposits and

  4. Properties of aftershock sequences in southern California

    Science.gov (United States)

    Kisslinger, Carl; Jones, Lucile M.

    1991-07-01

    The temporal behavior of 39 aftershock sequences in southern California, 1933-1988, was modeled by the modified Omori relation. Minimum magnitudes for completeness of each sequence catalog were determined, and the maximum likelihood estimates of the parameters K, p, and c, with the standard errors on each, were determined by the Ogata algorithm. The b value of each sequence was also calculated. Many of the active faults in the region, both strike slip and thrust, were sampled. The p values were graded in terms of the size of the standard error relative to the p value itself. Most of the sequences were modeled well by the Omori relation. Many of the sequences had p values close to the mean of the whole data set, 1.11±0.25, but values significantly different from the mean, as low as 0.7 and as high as 1.8, exist. No correlation of p with either the b value of the sequence or the mainshock magnitude was found. The results suggest a direct correlation of p values is with surface heat flow, with high values in the Salton Trough (high heat flow) and one low value in the San Bernardino Mountains and on the edge of the Ventura Basin (both low heat flow). The large fraction of the sequences with p values near the mean are at locations where the heat flow is near the regional mean, 74 mW/m2. If the hypothesis that aftershock decay rate is controlled by temperature at depth is valid, the effects of other factors such as heterogeneity of the fault zone properties are superimposed on the background rate determined by temperature. Surface heat flow is taken as an indicator of crustal temperature at hypocentral depths, but the effects on heat flow of convective heat transport and variations in near-surface thermal conductivity invalidate any simple association of local variations in heat flow with details of the subsurface temperature distribution. The interpretation is that higher temperatures in the aftershock source volume caused shortened stress relaxation times in the fault

  5. An Activity Catalogue of Southern Stars

    CERN Document Server

    Jenkins, J S; Tinney, C G; Butler, R P; McCarthy, C; Marcy, G W; Pinfield, D J; Carter, B D; Penny, A J

    2006-01-01

    We have acquired high-resolution echelle spectra of 225 F6-M5 type stars in the southern hemisphere. The stars are targets or candidates to be targets for the Anglo-Australian Planet Search. Ca$\\small{\\rmn{\\textsc{II}}}$~HK line cores were used to derive activity indices for all of these objects. The indices were converted to the Mt. Wilson system of measurements and log\\emph{R}$'_{\\rmn{HK}}$ values determined. A number of these stars had no previously derived activity indices. In addition we have also included the stars from Tinney et al. (2002) using our Mt. Wilson calibration. The radial-velocity instability (also known as jitter) level was determined for all 21 planet-host stars in our dataset. We find the jitter to be at a level considerably below the radial-velocity signatures in all but one of these systems. 19 stars from our sample were found to be active (log\\emph{R}$'_{\\rmn{HK}}$~$>$~-4.5) and thus have high levels of jitter. Radial-velocity analysis for planetary companions to these stars should pr...

  6. Milk production characteristics in Southern Brazil

    Directory of Open Access Journals (Sweden)

    Tony Picoli

    2015-07-01

    Full Text Available This paper aimed to describe milk production in Southern Rio Grande do Sul state, Brazil, and to identify factors that affect milk quality at this region. The average age of regional dairy farmers is 49, and 67.9% has not concluded elementary school. Dairy farming is carried out on properties with an average of 26.06 hectares and 8.4 lactating cows. Most of them (32.83% yield 50 to 100 L/day. Among the properties, 13.21% yield up to 30 L/day, and only 1.89% produces over 500 milk liters a day. Average yield was 6.8 L/day. Regarding to milking procedure, 39.3% farmers milk manually the animals, only 14.2% performed pre-milking teat disinfection, and 53.9% uses a single cloth to dry all animal teats. For infrastructure, 52.8% milks animals in wooden cowsheds. We observed that the average somatic cell count (SCC was within legal parameters, presenting negative correlation with milk production (r = -0.23 and lactose content (r = -0.39. However, the total bacterial count (TBC was above legal parameters. Education level seems to interfere in management and milk quality, because the less educated groups are, the less adequate are infrastructure, management, and product quality. Data show that there is a lower quality milk production where poor management techniques are adopted and owner education level affects milk quality.

  7. Southern Regional Center for Lightweight Innovative Design

    Energy Technology Data Exchange (ETDEWEB)

    Wang, Paul T. [Mississippi State Univ., Mississippi State, MS (United States)

    2012-12-01

    The Southern Regional Center for Lightweight Innovative Design (SRCLID) has developed an experimentally validated cradle-to-grave modeling and simulation effort to optimize automotive components in order to decrease weight and cost, yet increase performance and safety in crash scenarios. In summary, the three major objectives of this project are accomplished: To develop experimentally validated cradle-to-grave modeling and simulation tools to optimize automotive and truck components for lightweighting materials (aluminum, steel, and Mg alloys and polymer-based composites) with consideration of uncertainty to decrease weight and cost, yet increase the performance and safety in impact scenarios; To develop multiscale computational models that quantify microstructure-property relations by evaluating various length scales, from the atomic through component levels, for each step of the manufacturing process for vehicles; and To develop an integrated K-12 educational program to educate students on lightweighting designs and impact scenarios. In this final report, we divided the content into two parts: the first part contains the development of building blocks for the project, including materials and process models, process-structure-property (PSP) relationship, and experimental validation capabilities; the second part presents the demonstration task for Mg front-end work associated with USAMP projects.

  8. Eimeria infections in goats in Southern Portugal.

    Science.gov (United States)

    Silva, Liliana Machado Ribeiro da; Vila-Viçosa, Maria João Martins; Nunes, Telmo; Taubert, Anja; Hermosilla, Carlos; Cortes, Helder Carola Espiguinha

    2014-01-01

    Coccidiosis caused by Eimeria species is a major form of intestinal infection affecting intensively and semi-intensively reared goats. The province of Alentejo is the main goat-producing area in Portugal. Therefore, all 15 Serpentina goat farms in Alentejo were analyzed regarding the occurrence and diversity of Eimeria species. Fecal samples obtained from 144 animals (52.1% dairy goats, 47.9% pre-pubertal goats) were examined using the modified McMaster technique to determine the number of oocysts per gram of feces. Eimeria spp. oocysts were present in 98.61% of the fecal samples and, overall, nine different Eimeria species were identified. The most prevalent species were E. ninakohlyakimovae (88%) and E. arloingi (85%), followed by E. alijevi (63%) and E. caprovina (63%). The average number of oocysts shed was significantly lower in dairy goats than in pre-adult animals. Astonishingly, no clinical signs of coccidiosis were observed in any of the animals examined, even though they were shedding high numbers of oocysts and were infected with highly pathogenic species. Thus, implementation of routine diagnostic investigation of the occurrence and diversity of caprine Eimeria species may be a useful tool for determination and better understanding of their potential economic impact on goat herds in southern Portugal.

  9. PLEISTOCENE BATHYAL MOLLUSCAN ASSEMBLAGES FROM SOUTHERN ITALY

    Directory of Open Access Journals (Sweden)

    ITALO DI GERONIMO

    1997-11-01

    Full Text Available Four Pleistocene bathyal molluscan assemblages from southern Italy (Calabria and Messina area were studied. One hundred and thirty-six species were recorded. Twenty-four were classified and described in detail and thirty-five were illustrated. The following new combinations are pro posed: Solariella marginulata (Philippi, 1844, Iphitus tenuisculptus (Seguenza, 1876, Benthomangelia tenuicostata (Seguenza, 1879, Chrysallida microscalaria (Seguenza, 1876, Ennucula corbuloides (Seguenza, 1877, Ennucula rotundata (Seguenza, 1877, Thestyleda cuspidata (Philippi, 1844, Katadesmia confusa (Seguenza, 1877, Austrotindaria pusio (Philippi, 1844, Austrotindaria salicensis (Seguenza, 1877. Comments concerning the taxonomy of Fissurisepta Seguenza, 1862, Solariella Wood, 1842, Ennucula Iredale, 1931, Thestyleda Iredale, 1929, Ledella Verrill & Bush, 1897, Yoldiella Verrill & Bush, 1897, Bathyspinula Filatova, 1958, Katadesmia Dall, 1908, Austrotindaria Fleming, 1948 and Cadulus Philippi, 1844 are included. The assemblages are dominated by nuculoids and fit the general compositional pattern of the deep-sea molluscan communities. A paleodepth of 500-600 m is inferred for two assemblages, whereas a greater depth, pro bably not exceeding 1,000 m, is suggested for the other two. Taxonomic affinities with northeast Atlantic and more generally with World Ocean deep-sea molluscan faunas are remarkable. The Plio-Quaternary evolution of the deep Mediterranean benthos is discussed.    

  10. A Southern Ocean mode of multidecadal variability

    Science.gov (United States)

    Le Bars, D.; Viebahn, J. P.; Dijkstra, H. A.

    2016-03-01

    A 250 year simulation of a strongly eddying global version of the Parallel Ocean Program (POP) model reveals a new mode of intrinsic multidecadal variability, the Southern Ocean Mode (SOM), with a period of 40-50 year. The peak-to-peak difference in the global ocean heat content within a multidecadal cycle is up to 60 ZJ. This change results from surface heat flux variations in the South Atlantic and propagation of temperature anomalies along the Antarctic Circumpolar Current and into the Weddell gyre around 30°E. The temperature anomalies propagate as deep as 5000 m along the isopycnals between 50°S and 30°S and induce multidecadal changes in the Atlantic Meridional Overturning Circulation. A positive feedback loop between the generation of eddies through baroclinic instability and the dynamics of the mean circulation is essential for the existence of the SOM. The dominant physics appears similar to that responsible for variability found in a three-layer quasi-geostrophic eddy-resolving model. This combined with the fact that the SOM is not found in a noneddying version of the same global POP model further suggests that eddy processes are crucial for its existence and/or excitation.

  11. IFU spectroscopy of southern PNe: III

    CERN Document Server

    Ali, A; Basurah, H M; Amer, M A; Alsulami, R; Alruhaili, A

    2016-01-01

    In this paper we describe integral ?eld spectroscopic observations of four southern Galactic Planetary Nebulae (PNe), M3-4, M3-6, Hen2-29 and Hen2-37 covering the spectral range, 3400-7000?A.We derive the ionisation structure, the physical conditions, the chemical compositions and the kinematical characteristics of these PNe and ?nd good agreement with previous studies that relied upon the long-slit technique in their co-spatial area. From their chemical compositions as well as their spatial and kinematic characteristics, we determined that Hen2-29 is of the Peimbert Type I (He and N rich), while the other three are of Type II. The strength of the nebular He II line reveals that M3-3, Hen2-29 and Hen2-37 are of mid to high excitation classes while M3-6 is a low excitation planetary nebula (PN). A series of emission-line maps extracted from the data cubes were constructed for each PN to describe its overall structure. These show remarkable morphological diversity. Spatially resolved spectroscopy of M3-6, shows...

  12. Objectively classifying Southern Hemisphere extratropical cyclones

    Science.gov (United States)

    Catto, Jennifer

    2016-04-01

    There has been a long tradition in attempting to separate extratropical cyclones into different classes depending on their cloud signatures, airflows, synoptic precursors, or upper-level flow features. Depending on these features, the cyclones may have different impacts, for example in their precipitation intensity. It is important, therefore, to understand how the distribution of different cyclone classes may change in the future. Many of the previous classifications have been performed manually. In order to be able to evaluate climate models and understand how extratropical cyclones might change in the future, we need to be able to use an automated method to classify cyclones. Extratropical cyclones have been identified in the Southern Hemisphere from the ERA-Interim reanalysis dataset with a commonly used identification and tracking algorithm that employs 850 hPa relative vorticity. A clustering method applied to large-scale fields from ERA-Interim at the time of cyclone genesis (when the cyclone is first detected), has been used to objectively classify identified cyclones. The results are compared to the manual classification of Sinclair and Revell (2000) and the four objectively identified classes shown in this presentation are found to match well. The relative importance of diabatic heating in the clusters is investigated, as well as the differing precipitation characteristics. The success of the objective classification shows its utility in climate model evaluation and climate change studies.

  13. Alboran Basin, southern Spain - Part I: Geomorphology

    Energy Technology Data Exchange (ETDEWEB)

    Munoz, A. [Secretaria General de Pesca Maritima, Corazon de Maria, 8, 28002 Madrid (Spain); Ballesteros, M.; Rivera, J.; Acosta, J. [Instituto Espanol de Oceanografia, Corazon de Maria, 8, 28002 Madrid (Spain); Montoya, I. [Universidad Juan Carlos I, Campus de Mostoles, Madrid (Spain); Uchupi, E. [Woods Hole Oceanographic Institution, Woods Hole, MA 02543 (United States)

    2008-01-15

    Bathymetric, 3D relief and shaded relief maps created from multibeam echo-sounding data image the morphology of the Alboran Basin, a structural low along the east-west-trending Eurasian-African plates boundary. Topographic features in the basin are the consequence of volcanism associated with Miocene rifting, rift and post-rift sedimentation, and recent faulting resulting from the convergence of the African-Eurasian plates. Pleistiocene glacially induced regressions/transgressions when the sea level dropped to about 150 m below its present level gas seeps and bottom currents. Recent faulting and the Pleistocene transgressions/regressions led to mass-wasting, formation of turbidity currents and canyon erosion on the basin's slopes. Recent fault traces at the base of the northern basin slope have also served as passageways for thermogenic methane, the oxidation of which by bacteria led to the formation of carbonate mounds along the fault intercepts on the sea floor. Expulsion of thermogenic or biogenic gas has led to the formation of pockmarks; erosion by bottom currents has resulted in the formation of moats around seamounts and erosion of the seafloor of the Alboran Ridge and kept the southern edge of the 36 10'N high sediment free. (author)

  14. Active kinematics of the southern Red Sea

    Science.gov (United States)

    McClusky, S.; Reilinger, R.; Ogubazghi, G.; Amlesom, A.; Haileab, B.; Sholan, J.; Vernant, P.; Arrajehi, A.

    2009-04-01

    GPS measurements adjacent to the southern Red Sea and around the Afar Triple Junction (Red Sea Rift-Gulf of Aden Rift-East African Rift), indicate that the Red Sea rift bifurcates south of 16° N latitude with one branch following a continuation of the main Red Sea rift (~150° Azimuth) and the other oriented roughly N-S traversing the Danakil Depression/Afar volcanic province. These two rift branches account for the full Arabia-Nubia relative motion. Within the resolution of our observations, the partitioning of extension between rift branches varies linearly along strike; north of ~16°N extension (~15 mm/yr at 16°N) is confined to the main Red Sea rift while at the latitude of the Afar Triple Junction (~12°N) extension (~20 mm/yr) has transferred completely to the Danakil-Afar Depression. The Danakil block separates the two rifts and rotates counterclockwise, accommodating extension along the rifts and developing the triangular geometry of the Danakil/Afar Depression. Extrapolating the geodetic rates to the time of initial rifting of Arabia from Nubia (~25 ± 3 Ma) and estimating total extension across the rift branches from the present-day widths of the rifts suggests that Arabia-Nubia relative motion has been roughly constant since the initiation of Red Sea spreading, and that extension was initially confined to the main Red Sea rift with the presently observed bifurcation initiating at about 17 Ma.

  15. Southern African NGOs seize the initiative.

    Science.gov (United States)

    Morna, C L

    1990-11-01

    The Southern African Network of AIDS Service Organizations (SANASO) was begun at a conference in Harare, Zimbabwe in May 1990, marking the 1st local unification of Non-Governmental agencies in AIDS prevention and care. This region has about 20% of Africa's reported AIDS cases, but has seen an 8-fold increase in 2 years. The goals of the organization are to facilitate sharing of information, ideas and experiences, to promote cooperation among the NGOs, to move toward common positions on AIDS and to articulate to the rest of the world the needs of the region as regards AIDS. While the incidence ranges from 6/million in Lesotho, to 906/million in Malawi, the countries represented have varied but pressing political and economic situations making HIV infection a serious threat. Examples are economic contraction with reduced funding of health and education; rapid urbanization; wars in Angola and Mozambique, with associated sexual abuse, refugees and displaced people; and in South Africa Apartheid, unemployment, forced relocation, violence and even suspicion of the government's AIDS program. Discussions on how the NGOs in particular can help change peoples' behavior resulted in the suggestion that they involve People With AIDS (PWA), to give the disease "a face" and lessen fear and alienation. The best example of such a successful approach is the home-based care instituted by Zambia's Chikankata Salvation Army Mission Hospital.

  16. Dendroclimatic reconstructions for the southern Colorado plateau

    Energy Technology Data Exchange (ETDEWEB)

    Dean, J.S.; Funkhouser, G.S. [Univ. of Arizona, Tucson, AZ (United States)

    1995-09-01

    A geographical network of climate sensitive tree-ring chronologies consisting of 25 archaeological sequences and two bristlecone pine series provides the basis for high resolution reconstructions of low and high frequency climatic variability on the southern Colorado Plateau over the last 1,500 years. Qualitative and quantitative dendroclimatic analyses of these data produce annual retrodictions of yearly and seasonal precipitation and summer Palmer Drought Severity Indices for each station and reconstructions of regional scale patterns in climatic variability. These reconstructions provide detailed information on climatic fluctuations that affected biotic and human populations as well as long-term baseline data for evaluating present-day climate and estimating future climatic trends. When integrated with other measures of past environmental variability, these reconstructions specify periods of favorable and unfavorable environmental conditions that would have affected past human populations of the region. The severest degradation, which occurred between A.D. 1250 and 1450, probably was causally related to numerous cultural changes that occurred at the end of the l3th century including the Anasazi abandonment of the Four Comers area. Projecting environmental patterns that characterized the last two millennia into the future indicates potential hazards to long term uranium mill waste disposal and containment and the potential and limitations of environmental restoration.

  17. Late glacial aridity in southern Rocky Mountains

    Energy Technology Data Exchange (ETDEWEB)

    Davis, O.K.; Pitblado, B.L. [Univ. of Arizona, Tucson, AZ (United States)

    1995-09-01

    While the slopes of the present-day Colorado Rocky Mountains are characterized by large stands of subalpine and montane conifers, the Rockies of the late glacial looked dramatically different. Specifically, pollen records suggest that during the late glacial, Artemisia and Gramineae predominated throughout the mountains of Colorado. At some point between 11,000 and 10,000 B.P., however, both Artemisia and grasses underwent a dramatic decline, which can be identified in virtually every pollen diagram produced for Colorado mountain sites, including Como Lake (Sangre de Cristo Mountains), Copley Lake and Splains; Gulch (near Crested Butte), Molas Lake (San Juan Mountains), and Redrock Lake (Boulder County). Moreover, the same pattern seems to hold for pollen spectra derived for areas adjacent to Colorado, including at sites in the Chuska Mountains of New Mexico and in eastern Wyoming. The implications of this consistent finding are compelling. The closest modem analogues to the Artemisia- and Gramineae-dominated late-glacial Colorado Rockies are found in the relatively arid northern Great Basin, which suggests that annual precipitation was much lower in the late-glacial southern Rocky Mountains than it was throughout the Holocene.

  18. Solar cycle modulation of Southern Annular Mode

    Science.gov (United States)

    Kuroda, Yuhji

    2016-04-01

    Climate is known to be affected by various factors, including oceanic changes and volcanic eruptions. 11-year solar cycle change is one of such important factors. Observational analysis shows that the winter-mean North Atlantic Oscillation (NAO) and late-winter/spring Southern Annular Mode (SAM) show structural modulation associated with 11-year solar cycle. In fact, these signals tend to extend from surface to upper stratosphere and persistent longer period only in the High Solar (HS) years. In the present study, we used 35-year record of ERA-Interim reanalysis data and performed wave-energy and momentum analysis on the solar-cycle modulation of the SAM to examine key factors to create such solar-SAM relationship. It is found that enhanced wave-mean flow interaction tends to take place in the middle stratosphere in association with enhanced energy input from diabatic heating on September only in HS years. The result suggests atmospheric and solar conditions on September are keys to create solar-SAM relationship.

  19. Mechanization in firewood harvesting in southern Italy

    Directory of Open Access Journals (Sweden)

    2005-01-01

    Full Text Available The aim of this research was to survey current mechanization level of coppice harvesting in Southern Italy. The cooperation of the General Direction of the National Forest Service (NFS has been a basic tool of survey. A questionnaire compiled on purpose was sent to each Forest Station (hereinafter referred to as CS in the following regions: Basilicata, Campania and Calabria. A high percentage (80% of the CSs did fulfill the questionnaire. The answers highlight that: i the main assortment currently produced is firewood; ii the level of harvesting mechanization is rather low, equipment being quite obsolete: indeed, the most widely used machineries are farm tractors partly adapted to forest harvesting and equipped with cages or back winch; iii the use of animals for hauling (mules and oxen, the latter in Calabria is still quite frequent, while forest tractors, polyethylene chutes and cable cranes are almost absent; iv the use of individual protection (DPI and machinery protection devices (DPM is on average quite low.

  20. ICESat Observations of Southern Alaska Glaciers

    Science.gov (United States)

    Sauber, Jeanne; Molnia, Bruce F.; Mitchell, Darius

    2003-01-01

    In late February and March, 2003, the Ice, Cloud, and land Elevation Satellite (ICESat) measured ice and land elevations along profiles across southern Alaska. During this initial data acquisition stage ICESat observations were made on 8-day repeat tracks to enable calibration and validation of the ICESat data products. Each profile consists of a series of single point values derived from centroid elevations of an $\\approx$70 m diameter laser footprint. The points are s4pakated by $\\approx$172 m along track. Data siets of 8-day observations (an ascending and descending ground track) crossed the Bering and Malaspina Glacier. Following its 1993--1995 surge; the Bering Glacier has undergone major terminus retreat as well as ike thinning in the abtation zone. During the later part of the 20th century, parts of the Malaspina thinned by about 1 m/yr. The multiple observation profiles across the Bering and Malaspina piedmont lobes obtained in February/March are being geolocated on Landsat images and the elevation profiles will be used for a number o scientific objectives. Based on our simulations of ICESat performance over the varied ice surface of the Jakobshavn Glacier of GReenland, 2003, we expect to measure annual, and possibly seasonal, ice elevation changes on the large Alaskan glaciers. Using elevation data obtained from a second laser, we plan to estimate ice elevation changes on the Bering Glacier between March and October 2003.

  1. Southern Regional Center for Lightweight Innovative Design

    Energy Technology Data Exchange (ETDEWEB)

    None

    2012-08-24

    The Southern Regional Center for Lightweight Innovative Design (SRCLID) has developed an experimentally validated cradle-to-grave modeling and simulation effort to optimize automotive components in order to decrease weight and cost, yet increase performance and safety in crash scenarios. In summary, the three major objectives of this project are accomplished: To develop experimentally validated cradle-to-grave modeling and simulation tools to optimize automotive and truck components for lightweighting materials (aluminum, steel, and Mg alloys and polymer-based composites) with consideration of uncertainty to decrease weight and cost, yet increase the performance and safety in impact scenarios; To develop multiscale computational models that quantify microstructure-property relations by evaluating various length scales, from the atomic through component levels, for each step of the manufacturing process for vehicles; and To develop an integrated K-12 educational program to educate students on lightweighting designs and impact scenarios. In this final report, we divided the content into two parts: the first part contains the development of building blocks for the project, including materials and process models, process-structure-property (PSP) relationship, and experimental validation capabilities; the second part presents the demonstration task for Mg front-end work associated with USAMP projects.

  2. Wastewater effluent dispersal in Southern California Bays

    KAUST Repository

    Uchiyama, Yusuke

    2014-03-01

    The dispersal and dilution of urban wastewater effluents from offshore, subsurface outfalls is simulated with a comprehensive circulation model with downscaling in nested grid configurations for San Pedro and Santa Monica Bays in Southern California during Fall of 2006. The circulation is comprised of mean persistent currents, mesoscale and submesoscale eddies, and tides. Effluent volume inflow rates at Huntington Beach and Hyperion are specified, and both their present outfall locations and alternative nearshore diversion sites are assessed. The effluent tracer concentration fields are highly intermittent mainly due to eddy currents, and their probability distribution functions have long tails of high concentration. The dilution rate is controlled by submesoscale stirring and straining in tracer filaments. The dominant dispersal pattern is alongshore in both directions, approximately along isobaths, over distances of more than 10. km before dilution takes over. The current outfall locations mostly keep the effluent below the surface and away from the shore, as intended, but the nearshore diversions do not. © 2014 Elsevier Ltd.

  3. Hunting the Southern Skies with SIMBA

    Science.gov (United States)

    2001-08-01

    First Images from the New "Millimetre Camera" on SEST at La Silla Summary A new instrument, SIMBA ("SEST IMaging Bolometer Array") , has been installed at the Swedish-ESO Submillimetre Telescope (SEST) at the ESO La Silla Observatory in July 2001. It records astronomical images at a wavelength of 1.2 mm and is able to quickly map large sky areas. In order to achieve the best possible sensitivity, SIMBA is cooled to only 0.3 deg above the absolute zero on the temperature scale. SIMBA is the first imaging millimetre instrument in the southern hemisphere . Radiation at this wavelength is mostly emitted from cold dust and ionized gas in a variety of objects in the Universe. Among other, SIMBA now opens exciting prospects for in-depth studies of the "hidden" sites of star formation , deep inside dense interstellar nebulae. While such clouds are impenetrable to optical light, they are transparent to millimetre radiation and SIMBA can therefore observe the associated phenomena, in particular the dust around nascent stars . This sophisticated instrument can also search for disks of cold dust around nearby stars in which planets are being formed or which may be left-overs of this basic process. Equally important, SIMBA may observe extremely distant galaxies in the early universe , recording them while they were still in the formation stage. Various SIMBA images have been obtained during the first tests of the new instrument. The first observations confirm the great promise for unique astronomical studies of the southern sky in the millimetre wavelength region. These results also pave the way towards the Atacama Large Millimeter Array (ALMA) , the giant, joint research project that is now under study in Europe, the USA and Japan. PR Photo 28a/01 : SIMBA image centered on the infrared source IRAS 17175-3544 PR Photo 28b/01 : SIMBA image centered on the infrared source IRAS 18434-0242 PR Photo 28c/01 : SIMBA image centered on the infrared source IRAS 17271-3439 PR Photo 28d/01

  4. Holocene geologic slip rate for the Banning strand of the southern San Andreas Fault, southern California

    Science.gov (United States)

    Gold, Peter O.; Behr, Whitney M.; Rood, Dylan; Sharp, Warren D.; Rockwell, Thomas; Kendrick, Katherine J.; Salin, Aaron

    2015-01-01

    Northwest directed slip from the southern San Andreas Fault is transferred to the Mission Creek, Banning, and Garnet Hill fault strands in the northwestern Coachella Valley. How slip is partitioned between these three faults is critical to southern California seismic hazard estimates but is poorly understood. In this paper, we report the first slip rate measured for the Banning fault strand. We constrain the depositional age of an alluvial fan offset 25 ± 5 m from its source by the Banning strand to between 5.1 ± 0.4 ka (95% confidence interval (CI)) and 6.4 + 3.7/−2.1 ka (95% CI) using U-series dating of pedogenic carbonate clast coatings and 10Be cosmogenic nuclide exposure dating of surface clasts. We calculate a Holocene geologic slip rate for the Banning strand of 3.9 + 2.3/−1.6 mm/yr (median, 95% CI) to 4.9 + 1.0/−0.9 mm/yr (median, 95% CI). This rate represents only 25–35% of the total slip accommodated by this section of the southern San Andreas Fault, suggesting a model in which slip is less concentrated on the Banning strand than previously thought. In rejecting the possibility that the Banning strand is the dominant structure, our results highlight an even greater need for slip rate and paleoseismic measurements along faults in the northwestern Coachella Valley in order to test the validity of current earthquake hazard models. In addition, our comparison of ages measured with U-series and 10Be exposure dating demonstrates the importance of using multiple geochronometers when estimating the depositional age of alluvial landforms.

  5. Research Ship Southern Surveyor Underway Meteorological Data, Quality Controlled

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Research Ship Southern Surveyor Underway Meteorological Data (delayed ~10 days for quality control) are from the Shipboard Automated Meteorological and Oceanographic...

  6. European population substructure: clustering of northern and southern populations.

    Directory of Open Access Journals (Sweden)

    Michael F Seldin

    2006-09-01

    Full Text Available Using a genome-wide single nucleotide polymorphism (SNP panel, we observed population structure in a diverse group of Europeans and European Americans. Under a variety of conditions and tests, there is a consistent and reproducible distinction between "northern" and "southern" European population groups: most individual participants with southern European ancestry (Italian, Spanish, Portuguese, and Greek have >85% membership in the "southern" population; and most northern, western, eastern, and central Europeans have >90% in the "northern" population group. Ashkenazi Jewish as well as Sephardic Jewish origin also showed >85% membership in the "southern" population, consistent with a later Mediterranean origin of these ethnic groups. Based on this work, we have developed a core set of informative SNP markers that can control for this partition in European population structure in a variety of clinical and genetic studies.

  7. Vestalaria vinnula spec. nov. from southern Vietnam (Odonata: Calopterygidae)

    NARCIS (Netherlands)

    Hämäläinen, M.

    2006-01-01

    Vestalaria vinnula spec.nov. (holotype male, southern Vietnam, Lam Dong province, Blao, 1962) is described in both sexes and compared with other species of Vestalaria May, 1935 (= the Vestalis smaragdina - group), which is ranked as valid genus.

  8. Final Critical Habitat for Southern Resident Killer Whales

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — A geospatial data set depicting the boundaries of marine areas designated as critical habitat under the Endangered Species Act (ESA) for Southern Resident killer...

  9. Extra limit counts for southern sea otter 2016

    Data.gov (United States)

    U.S. Geological Survey, Department of the Interior — The GIS shapefile "Extra limit counts of southern sea otters 2016" is a point layer representing the locations of sea otter sightings that fall outside the...

  10. Southern Monterey Bay Littoral Cell CRSMP Proposed Receiver Site 2008

    Data.gov (United States)

    California Department of Resources — Given the location of the critical areas of erosion and the need to avoid adverse impacts to local sensitive habitat, the Southern Monterey Bay Coastal RSM Plan...

  11. Waterfowl breeding population survey: Southern Alberta: May 1991

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — This report summarizes the Waterfowl Breeding Population and Habitat Survey for southern Alberta during 1991. The primary purpose of the survey is to provide...

  12. The beginnings of the Southern Child/Pediatric Neurology Society.

    Science.gov (United States)

    Dyken, Paul Richard; Bodensteiner, John B

    2015-04-01

    The founding and early development of the Southern Pediatric Neurology Society was in many ways parallel to that of the Child Neurology Society. The organization started out as the Southern Child Neurology Society but the name was changed at the time of incorporation so as to avoid confusion of identity and purpose with the larger Child Neurology Society. Although there are archives of early days and the later development of the Southern Pediatric Neurology Society, the details have never been set down in a narrative explaining the events that led to the development of the organization. In this paper, we try to produce a written record of the history of the founding and early development of the Southern Pediatric Neurology Society.

  13. Killer Whale Genetic Data - Southern resident killer whale pedigree analysis

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — In this project, we are using genetic variation to infer mating patterns in the southern killer whale community. In Canada, this population was listed as threatened...

  14. Waterfowl breeding population survey: Southern and central Alberta: May 1995

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — This report summarizes the Waterfowl Breeding Population and Habitat Survey for southern and central Alberta during 1995. The primary purpose of the survey is to...

  15. Final critical habitat for Southern Pigtoe (Pleurobema georgianum)

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — This Polygon shapefile of critical habitat for the Southern Pigtoe (Pleurobema georgianum) based on the description provided in the Federal Register.

  16. Review: Current writing: Text and reception in Southern Africa

    Directory of Open Access Journals (Sweden)

    A. L. Combrink

    1990-05-01

    Full Text Available Current writing: Text and reception in Southern Africa. (Published by the University of Natal under the joint editorship of Margaret Lenta, Michael Chapman, Margaret Daymond and Johan U. Jacobs. Volume 1, 1989 - editor: Margaret Lenta

  17. [Experience with amalgams manufactured by Southern Dental Industries (Australia)].

    Science.gov (United States)

    Tarján, I; Gábris, K; Balaton, P; Dénes, J

    1990-09-01

    The gamma 2 phaseless amalgam of the firm Southern Dental Industries (SDI) was tested. None of the 205 fillings showed level deviations, unevenness, secondary caries and breakage. A minimum size gap was found in 2 cases.

  18. Final critical habitat for Southern Clubshell (Pleurobema decisum)

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — This Polygon shapefile of critical habitat for the Southern Clubshell (Pleurobema decisum) based on the description provided in the Federal Register.

  19. Southern Monterey Bay Littoral Cell CRSMP Sensitive Habitat 2008

    Data.gov (United States)

    California Department of Resources — One of the most important functions of the southern Monterey Bay coastal system is its role as a habitat for a unique flora and fauna. The beaches are habitat for...

  20. Final critical habitat for Southern Acornshell (Epioblasma othcaloogensis)

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — This Polygon shapefile of critical habitat for the Southern Acornshell (Epioblasma othcaloogensis) based on the description provided in the Federal Register.

  1. Southern Watersheds Common Reed Project, Final Report [Draft

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — The Southern Watersheds Common Reed Project is an interagency effort to demonstrate effective control of the invasive wetland plant, common reed, while...

  2. Status of southern James Bay population of Canada geese

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — This report provides background on the southern James Bay population (SJBP) of Canada geese, as well as a summary of problems confronting SJBP and recommendations...

  3. Southern Great Plains rapid ecoregional assessment : Pre-assessment report

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — The purpose of the Pre-Assessment Report for the Southern Great Plains Rapid Ecoregional Assessment (REA) is to document the selection process for and final list of...

  4. Avian botulism in the southern San Joaquin valley 1970

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — A joint effort of the Department of Fish and' Game and the U. S. Bureau of Sport Fisheries and Wildlife contained botulism losses in the southern San Joaquin Valley...

  5. Waterfowl production survey: Southern and central Alberta: July 1996

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — This report summarizes the Waterfowl Production and Habitat Survey for southern and central Alberta during 1996. The primary purpose of the survey is to provide...

  6. Climate Prediction Center Equatorial Southern Oscillation Index (1949-present)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This is one of the CPC?s Monthly Atmospheric and SST Indices. It contains Equatorial Southern Oscillation Index (standardized sea level pressure differences between...

  7. The Pali Aike Windstreak Field, Southern Patagonia, Argentina

    Science.gov (United States)

    Rodriguez, J. A. P.; Zimbelman, J. R.; Kargel, J. S.; Tanaka, K. L.; Yamamoto, A.; Sasaki, S.

    2008-03-01

    Windstreaks are amongst the most common aeolian landforms on Mars, yet they are extremely rare on Earth. We have identified and carried out field based observations of an outstanding zone of windstreaks in southern Patagonia Argentina.

  8. Implications of the genetic epidemiology of globin haplotypes linked to the sickle cell gene in southern Iran.

    Science.gov (United States)

    Rahimi, Zohreh; Merat, Ahmad; Gerard, Nathalie; Krishnamoorthy, Rajagopal; Nagel, Ronald L

    2006-12-01

    To determine the origin of sickle cell mutation in different ethnic groups living in southern Iran, we studied the haplotype background of the betaS and betaA genes in subjects from the provinces of Fars, Khuzestan, Bushehr, Hormozgan, and Kerman and from the islands of Khark and Qeshm. beta-globin gene cluster haplotypes were determined using the PCR-RFLP technique. Detection of -alpha 3.7 deletion and beta-thalassemia mutations were defined by PCR and reverse dot blot techniques, respectively. The framework of the beta-globin gene was determined using denaturing gradient gel electrophoresis. We found that the betaS mutation in southern Iran is associated with multiple mutational events. Most of the patients were from two ethnic groups: Farsi speakers (presumably Persian in origin) from Fars province and patients of Arab origin from Khuzestan province. In both ethnic groups the Arab-Indian haplotype was the most prevalent. The frequencies of the Arab-Indian and African haplotypes in sickle cell anemia patients from the provinces of Fars and Khuzestan were similar. Among betaA chromosomes the Bantu A2 haplotype was the most prevalent. The decrease in alpha-globin production in SS patients and AS individuals appeared to be related to the reduction in mean cell volume and mean cell hemoglobin. The Arab-Indian haplotype gene flow into this region of Iran can be traced to the Sassanian Empire. It is likely that the influx of betaS genes linked to the Benin and Bantu haplotypes, of African origin, must have occurred during the Arab slave trade.

  9. Hydrothermal system in Southern Grass Valley, Pershing County, Nevada

    Energy Technology Data Exchange (ETDEWEB)

    Welch, A.H.; Sorey, M.L.; Olmsted, F.H.

    1981-01-01

    Southern Grass Valley is a fairly typical extensional basin in the Basin and Range province. Leach Hot Springs, in the southern part of the valley, represents the discharge end of an active hydrothermal flow system with an estimated deep aquifer temperature of 163 to 176/sup 0/C. Results of geologic, hydrologic, geophysical and geochemical investigations are discussed in an attempt to construct an internally consistent model of the system.

  10. Operational Practice and Prospects of China Southern Power Grid

    Institute of Scientific and Technical Information of China (English)

    Wang Jiuling; Wang Ben

    2006-01-01

    @@ The Applied Research on Higher Voltage Class for China Southern Power Grid completed at the end of 2004 pointed to a conclusion that transmission schemes using ±800-kV DC and 1000-kV AC are feasible to the southern grid. According to the grid's 11th Five-Year Plan, the ultra-high voltage transmission will take its place in the power grid soon.

  11. A synopsis of Peristrophe (Acanthaceae in southern Africa

    Directory of Open Access Journals (Sweden)

    K. Balkwill

    1996-12-01

    Full Text Available A synopsis of Peristrophe Nees (Acanthaceae in southern Africa is provided. Peristrophe comprises nine species, one with two subspecies in southern Africa. A key for identification and descriptions of species not included in recent literature are provided. Morphology of tertiary bracts,indumentum on stems and distribution is illustrated and diagnostic characters, distribution, habitat, flowering  time and conservation status are discussed for each species.

  12. The Molonglo Southern 4 Jy sample (MS4). I. Definition

    CERN Document Server

    Burgess, A M

    2005-01-01

    We have defined a complete sample of 228 southern radio sources at 408 MHz with integrated flux densities S_408 > 4.0 Jy, Galactic latitude |b| > 10 deg and declination -85 deg 10.9 Jy (SMS4), provides a southern sample closely equivalent to the well-studied northern 3CRR sample. Comparison of SMS4 with 3CRR shows a reassuring similarity in source density and median flux density between the two samples.

  13. Wetland vegetation of southern KwaZulu-Natal, South Africa

    Directory of Open Access Journals (Sweden)

    L. Perkins

    2000-12-01

    Full Text Available Vegetation data from southern KwaZulu-Natal were analysed. TWINSPAN classification separated the entire data set (547 releves into five subsets. One subset, representing the vegetation of the wetlands of southern Kw aZulu-Natal, was further classified by Braun-Blanquet procedures. Eight plant communities and fourteen subcommunities were identified and described. The results can be integrated with existing phytosociological classifications of the Grassland Biome.

  14. The hydrothermal system in southern Grass Valley, Pershing County, Nevada

    Science.gov (United States)

    Welch, Alan H.; Sorey, M.L.; Olmsted, F.H.

    1981-01-01

    Southern Grass Valley is typical extensional basin in the Basin and Range province. Leach Hot Springs, in the southern part of the valley, represents the discharge end of an active hydrothermal flow system with an estimated deep aquifer temperature of 163-173C. This report discusses results of geologic, hydrologic, geophysical and geochemical investigations used in an attempt to construct an internally consistent model of the system. (USGS)

  15. Southern Philippines Public Perception Survey Exploration and Analysis

    Science.gov (United States)

    2013-07-31

    23 Table 3. Factor Analysis Results for Cotabato. The dark shaded region of the tables indicates common factors with common loadings, the lighter... tourism , security, etc. It is home to Western Mindanao Command and some of the Philippine Air Forces. It also enjoys reliable power most of the time...Philippines as part of a widespread Islamic state which would span Indonesia, Malaysia , southern Thailand, Singapore, Brunei, and the southern Philippines

  16. 77 FR 31004 - Southern Natural Gas Company; Notice of Request Under Blanket Authorization

    Science.gov (United States)

    2012-05-24

    ...] Southern Natural Gas Company; Notice of Request Under Blanket Authorization Take notice that on May 9, 2012, Southern Natural Gas Company (Southern), 569 Brookwood Village, Suite 501, Birmingham, Alabama 35209, filed... Commission's regulations under the Natural Gas Act (NGA), and Southern's blanket certificate issued in...

  17. 76 FR 66054 - Southern Natural Gas Company, L.L.C.; Notice of Application

    Science.gov (United States)

    2011-10-25

    ... Energy Regulatory Commission Southern Natural Gas Company, L.L.C.; Notice of Application Take notice that on October 7, 2011, Southern Natural Gas Company, L.L.C. (Southern), 569 Brookwood Village, Suite 501... application should be directed to Glenn A. Sheffield, Director--Rates and Regulatory, Southern Natural...

  18. 78 FR 14400 - Southern USA Resources, Inc., Order of Suspension of Trading

    Science.gov (United States)

    2013-03-05

    ... COMMISSION Southern USA Resources, Inc., Order of Suspension of Trading March 1, 2013. It appears to the... securities of Southern USA Resources, Inc. (``Southern USA'') because of questions regarding the accuracy of... company's outstanding shares. Southern USA's securities are quoted on the OTC Link, operated by...

  19. Characteristics of cyclone climatology and variability in the Southern Ocean

    Institute of Scientific and Technical Information of China (English)

    WEI Lixin; QIN Ting

    2016-01-01

    A new climatology of cyclones in the Southern Ocean is generated by applying an automated cyclone detection and tracking algorithm (developed by Hodges at the Reading University) for an improved and relatively high-resolution European Centre for Medium-Range Weather Forecasts atmospheric reanalysis during 1979–2013. A validation shows that identified cyclone tracks are in good agreement with a available analyzed cyclone product. The climatological characteristics of the Southern Ocean cyclones are then analyzed, including track, number, density, intensity, deepening rate and explosive events. An analysis shows that the number of cyclones in the Southern Ocean has increased for 1979–2013, but only statistically significant in summer. Coincident with the circumpolar trough, a single high-density band of cyclones is observed in 55°–67°S, and cyclone density has generally increased in north of this band for 1979–2013, except summer. The intensity of up to 70% cyclones in the Southern Ocean is less than 980 hPa, and only a few cyclones with pressure less than 920 hPa are detected for 1979–2013. Further analysis shows that a high frequency of explosive cyclones is located in the band of 45°–55°S, and the Atlantic Ocean sector has much higher frequent occurrence of the explosive cyclones than that in the Pacific Ocean sector. Additionally, the relationship between cyclone activities in the Southern Ocean and the Southern Annular Mode is discussed.

  20. Physical properties of Southern infrared dark clouds

    Science.gov (United States)

    Vasyunina, T.; Linz, H.; Henning, Th.; Stecklum, B.; Klose, S.; Nyman, L.-Å.

    2009-05-01

    Context: What are the mechanisms by which massive stars form? What are the initial conditions for these processes? It is commonly assumed that cold and dense Infrared Dark Clouds (IRDCs) represent the birth-sites of massive stars. Therefore, these clouds have been receiving an increasing amount of attention, and their analysis offers the opportunity to tackle the afore mentioned questions. Aims: To enlarge the sample of well-characterised IRDCs in the southern hemisphere, where ALMA will play a major role in the near future, we have developed a program to study the gas and dust of southern infrared dark clouds. The present paper attempts to characterize the continuum properties of this sample of IRDCs. Methods: We cross-correlated 1.2 mm continuum data from SIMBA bolometer array mounted on SEST telescope with Spitzer/GLIMPSE images to establish the connection between emission sources at millimeter wavelengths and the IRDCs that we observe at 8 μm in absorption against the bright PAH background. Analysing the dust emission and extinction enables us to determine the masses and column densities, which are important quantities in characterizing the initial conditions of massive star formation. We also evaluated the limitations of the emission and extinction methods. Results: The morphology of the 1.2 mm continuum emission is in all cases in close agreement with the mid-infrared extinction. The total masses of the IRDCs were found to range from 150 to 1150 M_⊙ (emission data) and from 300 to 1750 M_⊙ (extinction data). We derived peak column densities of between 0.9 and 4.6 × 1022 cm-2 (emission data) and 2.1 and 5.4 × 1022 cm-2 (extinction data). We demonstrate that the extinction method is unreliable at very high extinction values (and column densities) beyond AV values of roughly 75 mag according to the Weingartner & Draine (2001) extinction relation RV = 5.5 model B (around 200 mag when following the common Mathis (1990, ApJ, 548, 296) extinction calibration

  1. Neogene foreland tectonics in the southern Appenines

    Energy Technology Data Exchange (ETDEWEB)

    Roure, F.; Casero, P.; Moretti, I.; Mueller, C.; Sage, L.; Vially, R. (Institut Francais du Petrole, Rueil-Malmaison)

    1988-08-01

    Combined structural and biostratigraphic analyses and seismic interpretation help them to balance cross sections through the southern Apennines from the Adriatic to the Tyrrhenian Sea and to propose an overall model for the evolution of the belt. Three lithostratigraphic units have been distinguished according to their Mesozoic facies and style of deformation: the western platform (upper unit), the Lagonegro-Molise basin, and the eastern platform. Foreland deformation migrated from west to east, and external domains were reached progressively by synorogenic flysch deposits (foredeep) and later incorporated into the thrust sheets. Presently, only the most external part of the eastern platform is still unaffected by thrusting, while internal parts are building the overthrust belt at depth, which is masked on the surface by allochthonous basinal nappes. The evolutive geometry of thrust and piggy-back basins results from the continuous understacking of new material at the bottom of the tectonic prism. The deeper basement is also progressively involved in the deformation, giving rise to large nappe anticlines. Despite the early subsidence and deformation of the western platform and basinal domains in Langhian to Tortonian time, all the deformation of the eastern platform has occurred since Messinian time. These compressive structures are thus contemporaneous with the opening of the Tyrrhenian Sea. To the west, the upper tectonic units of the Apennines are indeed affected by listric normal faulting, with previous thrust planes having been locally reactivated during the distension. Post-Messinian shortening in the sedimentary cover is accompanied by a crustal thickening outlined by the Moho's geometry. The authors interpret it as a result of the subduction of the Apulian continental lithosphere. Recent uplift of the Apennines is indeed directly related to this crustal root.

  2. Tuberculosis in a southern Brazilian prison

    Directory of Open Access Journals (Sweden)

    Daniele Kuhleis

    2012-11-01

    Full Text Available The occurrence of tuberculosis (TB in prisons has been described as an alarming public health problem in many countries, especially in developing nations. The objective of this study was to conduct a survey among prisoners with TB respiratory symptoms in order to estimate the incidence of the disease, to analyze the drug susceptibility profile and genotype the isolates of Mycobacterium tuberculosis in the city of Charqueadas, southern of Brazil. The TB incidence was 55/1,900 inhabitants in the prison; this corresponds to an incidence of 3,789/100,000 inhabitants, with a prevalence of 72/1,900 (4,960/100,000 inhabitants. Drug susceptibility test was performed and, among the analyzed isolates, 85% were susceptible to all drugs tested and 15% were resistant to at least one drug, of which 89% were resistant only to isoniazid (INH or in combination with another drug. The genotype classification of spoligotyping analysis showed that 40% of the isolates belong to LAM family, 22% to T family, 17.5% to Haarlem family, 12.5% to U family and 3% to X family. The shared international spoligotypes most frequently found were 729 (27%, 50 (9.5%, 42 (8%, 53 (8% and 863 (8%. In conclusion, it was observed that TB in this specific population had been caused, mostly, by strains that have been transmitted in the last few years, as demonstrated by the large level of genotype clustering. In addition, it was found specific large clusters, which were not often found in the general population from the same period and in the same region.

  3. Cephalopods distribution in the southern Aegean Sea

    Directory of Open Access Journals (Sweden)

    E. LEFKADITOU

    2012-12-01

    Full Text Available The present study concerns faunal composition and distribution of cephalopods in the southern Aegean Sea (35Ζ 13’ 19’’- 37Ζ 55’ 25’’ N, 23Ζ 00’ 15’’ - 28Ζ 15’ 37’’ E. Samples were collected from 708 hauls, obtained by an experimental bottom trawl net during eight surveys carried out in the summers of the years 1994-2001, as well as by commercial trawl net during four surveys carried out in September 1995, December 1995, May and September 1996. The hauls were performed at depths ranging from 16 to 778 m. A total of 34 species of cephalopod in 12 families were identified, including 11 oegopsid squid, 3 myopsid squid, 7 octopod, 3 cuttlefish and 10 sepiolid. Trawling with the experimental net resulted in the capturing of some uncommon pelagic species, such as Ctenopteryx siculaand Octopoteuthis sicula, which were recorded for the first time in the Aegean Sea. Most of the species showed a wide depth and geographical range. The species: Sepia officinalis, Sepietta neglecta, Sepietta obscuraand Sepiola rondeletiwere caught only on the continental shelf, whereas the Ancistroteuthis lichtensteini, Bathypolypous sponsalis, Brachioteuthis riisei, Chiroteuthis veranyi, Ctenopteryx sicula, Heteroteuthis dispar, Histioteuthis reversa, Neorossia caroli and Pyroteuthis margaritiferawere found only on the slope. The rest of the species extended in both continental shelf and slope. The spatial distribution of different species groups is discussed in relation to the hydrology and topography of the study area and the species ecology.

  4. URBAN WATERSHED STUDIES IN SOUTHERN BRAZIL

    Directory of Open Access Journals (Sweden)

    Cristiano Poleto

    2007-12-01

    Full Text Available One of the greatest problems observed in Brazilian urban watersheds are concerned to the amount of solid residues, domestic sewerage and sediments that are disposed in the rivers and streams that drain those areas. This project aims to present these problems through a study of case taken in an urban watershed in Porto Alegre city, Southern Brazil. For this study, different procedures were used, such as field surveys, interviews with the inhabitants, satellite images, sediment samples, flow measures and morphology assessment of part of the local fluvial system to check the degree of instability of the channel. In 2005, it was verified that 42.57% of the watershed was impermeable, considering the paved streets, the residential and commercial buildings and stone pavements. As there was no sewer treatment, most of this sewerage was directly disposed into the stream and the TOC has reached 20% (m/m. Moreover, the occupation of riparian areas, a great amount of soil exposed in the watershed, the nonpaved streets and a great volume of solid residues were causing the instability in the channel, silting the stream bed. The metals (Zn, Pb and Cr selected for this study are most frequently found in high concentrations in urban areas. The results suggest the occurrence of a high enrichment of the fluvial sediment by these metals. The concentrations of these elements vary temporally during storms due to the input of impervious area runoff containing high concentration of elements associated to vehicular traffic and other anthropogenic activities. Then, it is possible to conclude that the contamination of the urban watershed is reflected in the results obtained in the fluvial suspended sediments.

  5. URBAN WATERSHED STUDIES IN SOUTHERN BRAZIL

    Directory of Open Access Journals (Sweden)

    Cristiano Poleto

    2007-01-01

    Full Text Available One of the greatest problems observed in Brazilian urban watersheds are concerned to the amount of solid residues, domestic sewerage and sediments that are disposed in the rivers and streams that drain those areas. This project aims to present these problems through a study of case taken in an urban watershed in Porto Alegre city, Southern Brazil. For this study, different procedures were used, such as field surveys, interviews with the inhabitants, satellite images, sediment samples, flow measures and morphology assessment of part of the local fluvial system to check the degree of instability of the channel. In 2005, it was verified that 42.57% of the watershed was impermeable, considering the paved streets, the residential and commercial buildings and stone pavements. As there was no sewer treatment, most of this sewerage was directly disposed into the stream and the TOC has reached 20% (m/m. Moreover, the occupation of riparian areas, a great amount of soil exposed in the watershed, the nonpaved streets and a great volume of solid residues were causing the instability in the channel, silting the stream bed. The metals (Zn, Pb and Cr selected for this study are most frequently found in high concentrations in urban areas. The results suggest the occurrence of a high enrichment of the fluvial sediment by these metals. The concentrations of these elements vary temporally during storms due to the input of impervious area runoff containing high concentration of elements associated to vehicular traffic and other anthropogenic activities. Then, it is possible to conclude that the contamination of the urban watershed is reflected in the results obtained in the fluvial suspended sediments.

  6. Phenological changes in the southern hemisphere.

    Directory of Open Access Journals (Sweden)

    Lynda E Chambers

    Full Text Available Current evidence of phenological responses to recent climate change is substantially biased towards northern hemisphere temperate regions. Given regional differences in climate change, shifts in phenology will not be uniform across the globe, and conclusions drawn from temperate systems in the northern hemisphere might not be applicable to other regions on the planet. We conduct the largest meta-analysis to date of phenological drivers and trends among southern hemisphere species, assessing 1208 long-term datasets from 89 studies on 347 species. Data were mostly from Australasia (Australia and New Zealand, South America and the Antarctic/subantarctic, and focused primarily on plants and birds. This meta-analysis shows an advance in the timing of spring events (with a strong Australian data bias, although substantial differences in trends were apparent among taxonomic groups and regions. When only statistically significant trends were considered, 82% of terrestrial datasets and 42% of marine datasets demonstrated an advance in phenology. Temperature was most frequently identified as the primary driver of phenological changes; however, in many studies it was the only climate variable considered. When precipitation was examined, it often played a key role but, in contrast with temperature, the direction of phenological shifts in response to precipitation variation was difficult to predict a priori. We discuss how phenological information can inform the adaptive capacity of species, their resilience, and constraints on autonomous adaptation. We also highlight serious weaknesses in past and current data collection and analyses at large regional scales (with very few studies in the tropics or from Africa and dramatic taxonomic biases. If accurate predictions regarding the general effects of climate change on the biology of organisms are to be made, data collection policies focussing on targeting data-deficient regions and taxa need to be financially

  7. Acute fasciolosis in cattle in southern Brazil

    Directory of Open Access Journals (Sweden)

    Maria de Lourdes Adrien

    2013-06-01

    Full Text Available This study describes the epidemiological and pathological aspects of an outbreak of acute fasciolosis in cattle in southern Brazil. Fifteen out of 70 three-year-old pregnant cows lost weight in the 30-40 days prior to calving. Clinical signs included diarrhea, weakness, mild anemia and jaundice. Dark yellow fluid in the abdominal cavity was observed at necropsy. Fibrin and clotted blood were adhered to the pericardium and lung, primarily in the diaphragmatic lobes. The liver was enlarged, and the capsular surface was irregular with clear areas and petechiae. At the cut surface, the liver was irregular, firm and edematous, and several hemorrhagic channels could be observed. Areas of fibrosis through the parenchyma and whitish thrombi occluding the great vessels were also observed. The livers of 10 cows that not died were condemned at slaughter for lesions of fasciolosis similar to those observed at necropsy. Microscopically, the liver showed areas of coagulation necrosis, extensive hemorrhages in the streaks or foci and disruption of the parenchyma with neutrophil and eosinophil infiltration. Fibrosis and bile duct proliferation were also observed. Immature Fasciola hepatica flukes were observed in the parenchyma surrounded by degenerated hepatocytes, neutrophils, eosinophils, and hemorrhages. The outbreak occurred on a farm located in an area endemic for fasciolosis, although the acute form of the disease is not common in cattle in this region. It is likely that the cows were infected by F. hepatica metacercariae released in the late fall or early spring in the rice stubble where the herd was grazing prior to calving. Although mortality due to fasciolosis in cattle is infrequent, outbreaks can occur and treatments that are effective in both the immature and adult forms of the parasite should be administered to prevent economic losses.

  8. Ultraviolet Index measurements in Southern Bahia, Brazil

    Science.gov (United States)

    de Paula Corrêa, Marcelo; Morégula, Andréa; Fausto, Agnes; Okuno, Emico; Mol, Anderson; Santos, João C.

    2009-03-01

    This work presents results of the Joint Project for Solar UV Index Measurements in Southern Bahia State (PIU Project), and represents the first effort to measure UV radiation in this tourist region. The project goal is twofold: it has a scientific component in which the distribution of UV Index will be mapped out, and it also intends to disseminate the data as a mean of divulgation of the UV Index levels to the population. This constitutes a meaningful effort to reduce health risks from excessive solar radiation exposure in a country that reports more than 120,000 new skin cancer cases each year. This project is within the framework of a larger initiative to studying UV index distribution in Brazilian regions. PIU project has had two phases: 1) seasonal measurements were performed during the summer and winter seasons of 2006 and 2007 on a sandy beach in Ilhéus (15,0° S; 39,0° W; sea level), as well as on an asphalt surface in the urban perimeter of Itabuna (14,8° S; 39,3° W; 54 m ASL); and 2) since October 2007, measurements have been continuously conducted on a concrete surface, about 20 km from the beach, in a urban area of Ilhéus. During the summer season, UV Index reaches extreme values (>11), and in winter, results range between high (6

  9. The Southern Nevada Agency Partnership Science and Research Synthesis: Science to support land management in Southern Nevada

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — This synthesis provides information related to the Southern Nevada Agency Partnership (SNAP) Science and Research Strategy Goal 1 – to restore, sustain and enhance...

  10. The Southern Nevada Agency Partnership Science and Research Synthesis: Science to support land management in Southern Nevada Executive Summary.

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — This synthesis provides information related to the Southern Nevada Agency Partnership (SNAP) Science and Research Strategy Goal 1 – to restore, sustain and enhance...

  11. Natural hazards at the southern margin of the Central Anatolian Plateau (CAP) (southern Turkey): Tsunami evidence

    Science.gov (United States)

    Ogretmen, Nazik; Cosentino, Domenico; Gliozzi, Elsa; Cipollari, Paola; Radeff, Giuditta; Yıldırım, Cengiz

    2016-04-01

    In regions that are located in steep, orogenic plateau margins, such as the coastal area of the Central Anatolian Plateau (CAP) southern margin, natural hazard studies related to active tectonics and events that are triggered by active tectonics (e.g., earthquakes, landslides, tsunamis) are very essential in the context of preventing possible damages. This work herein, represents some evidence of the tsunami hazard along the coast between Aydıncık and Narlıkuyu, in southern Turkey. The work is based on a study on out-of-place beachrock-slab boulder acummulation in Aydıncık district, which were transported onshore by sliding process, and on out-of-place more rounded boulders that were transported by saltation process in Narlıkuyu and Yeşilovacık districts. The presence of intertidal organisms (e.g., lithophaga boring, balanids, oysters, etc.) encrusting the boulders of both localities shows that those boulders were carried onland from a marine environment. According to their dimensions and weight, in agreement with out-of-place boulders from areas surely affected by tsunamis, those out-of-place boulders here are interpreted as due to tsunami waves. The tsunamites in the Aydıncık area are located on beachrock slabs. They are platty and some of those blocks are embricated and oriented perpendicular to the shoreline (NE-SW direction). Those boulders have been interpreted as transported by sliding process, in relation with the coastal morphology and the boulder geometry, which means that to move those boulders the energy of the tsunami not necessarily should have been as high as in saltation or rolling transport processes. On the contrary, in Narlıkuyu and Yeşilovacık localities, the boulders are well-rounded and ellipsoidal shaped, suggesting that they were transported by rolling and/or saltation mode rather than by sliding. To carry onland the tsunami boulders observed in the Narlıkuyu and Yeşilovacık districts, which in the Yeşilovacık area they are

  12. Exposure to ticks and seroprevalence of [i]Borrelia burgdorferi [/i]among a healthy young population living in the area of southern Podlasie, Poland

    Directory of Open Access Journals (Sweden)

    Anna Pańczuk

    2014-09-01

    Full Text Available [b]Objectives[/b]. The objective of the study was assessment of risk of infection with [i]Borrelia burgdorferi[/i] in the area of southern Podlasie in Poland, near the border with Belarus, by analysis of post-exposure procedure, and evaluation of asymptomatic infection in adolescents bitten by a tick, confirmed by serologic tests. [b]Material and methods[/b]. The study was conducted among 128 healthy individuals aged 16–20 who declared being bitten by a tick. The level of IgM and IgG class antibodies was determined using the immunoenzymatic test (Borrelia 14 kD + OspC IgM ELISA and Borrelia IgG + VlsE ELISA, DRG Diagnostics. Positive and doubtful results were confirmed using the Western blot method (EUROLINE-WB, EUROIMMUN. [b]Results[/b]. In the study group, the largest number of respondents (59.4% declared tick bite in the region of the lower extremities, most often in the knee pit. Among the methods for removing the tick the largest number of respondents indicated removing it with the use of tweezers, with a simple, swift steady movement (29.7%, and pulling it out with the fingers (22.7%. In the ELISA test, a positive or doubtful result in at least one class was observed in 25.0% of respondents (n=32/128: in IgM class – 23.4% (n=30/128, and in IgG class – 4.7% (n=6/128. After verification with the Western blot test, infection was confirmed in 5.5% of respondents (n=7/128: in IgM class – 1.6% (n=2/128, in IgG class – 3.9% (n=5/128. In IgM class antibodies, the Western blot test confirmed positive or doubtful results of the ELISA test in 6.7%, while in IgG class antibodies in 83.3%. [b]Conclusion[/b]. Evaluation of the actual infection with [i]Borrelia spp.[/i] using serologic tests is difficult due to a certain non-specificity of the ELISA test, especially in IgM class antibodies, and difficulties with performance of a wide scope of specific Western blot tests. The variety of methods of tick removal declared by adolescents suggests

  13. Serosurvey of Borrelia in dogs, horses, and humans exposed to ticks in a rural settlement of southern Brazil

    Directory of Open Access Journals (Sweden)

    Denise Amaral Gomes Nascimento

    Full Text Available Abstract The aims of the present study were to serosurvey dogs, horses, and humans highly exposed to tick bites for anti-Borrelia burgdorferi s.l. antibodies, identify tick species present, and determine risk factors associated with seropositivity in a rural settlement of Paraná State, southern Brazil. Eighty-seven residents were sampled, along with their 83 dogs and 18 horses, and individual questionnaires were administered. Immunofluorescence antibody test (IFAT was performed on serum samples and positive samples were subjected to western blot (WB analysis. Anti-B. burgdorferi antibodies were found in 4/87 (4.6% humans, 26/83 (31.3% dogs, and 7/18 (38.9% horses by IFAT, with 4/4 humans also positive by WB. Ticks identified were mostly from dogs and included 45/67 Rhipicephalus sanguineus, 21/67 Amblyomma ovale, and 1/67 A. cajennense sensu lato. All (34/34 horse ticks were identified as A. cajennense s.l.. No significant association was found when age, gender, or presence of ticks was correlated to seropositivity to Borrelia sp. In conclusion, although anti-Borrelia antibodies have been found in dogs, horses and their owners from the rural settlement, the lack of isolation, molecular characterization, absence of competent vectors and the low specificity of the commercial WB kit used herein may have impaired risk factor analysis.

  14. Southern Nevada Alternative Fuels Demonstration Project

    Energy Technology Data Exchange (ETDEWEB)

    Hyde, Dan; Fast, Matthew

    2009-12-31

    The Southern Nevada Alternative Fuels Program is designed to demonstrate, in a day-to-day bus operation, the reliability and efficiency of a hydrogen bus operation under extreme conditions. By using ICE technology and utilizing a virtually emission free fuel, benefits to be derived include air quality enhancement and vehicle performance improvements from domestically produced, renewable energy sources. The project objective is to help both Ford and the City demonstrate and evaluate the performance characteristics of the E-450 H2ICE shuttle buses developed by Ford, which use a 6.8-liter supercharged Triton V-10 engine with a hydrogen storage system equivalent to 29 gallons of gasoline. The technology used during the demonstration project in the Ford buses is a modified internal combustion engine that allows the vehicles to run on 100% hydrogen fuel. Hydrogen gives a more thorough fuel burn which results in more power and responsiveness and less pollution. The resultant emissions from the tailpipe are 2010 Phase II compliant with NO after treatment. The City will lease two of these E-450 H2ICE buses from Ford for two years. The buses are outfitted with additional equipment used to gather information needed for the evaluation. Performance, reliability, safety, efficiency, and rider comments data will be collected. The method of data collection will be both electronically and manually. Emissions readings were not obtained during the project. The City planned to measure the vehicle exhaust with an emissions analyzer machine but discovered the bus emission levels were below the capability of their machine. Passenger comments were solicited on the survey cards. The majority of comments were favorable. The controllable issues encountered during this demonstration project were mainly due to the size of the hydrogen fuel tanks at the site and the amount of fuel that could be dispensed during a specified period of time. The uncontrollable issues encountered during this

  15. Flood of April 2007 in Southern Maine

    Science.gov (United States)

    Lombard, Pamela J.

    2009-01-01

    Up to 8.5 inches of rain fell from April 15 through 18, 2007, in southern Maine. The rain - in combination with up to an inch of water from snowmelt - resulted in extensive flooding. York County, Maine, was declared a presidential disaster area following the event. The U.S. Geological Survey, in cooperation with the Federal Emergency Management Agency (FEMA), determined peak streamflows and recurrence intervals at 24 locations and peak water-surface elevations at 63 sites following the April 2007 flood. Peak streamflows were determined with data from continuous-record streamflow-gaging stations where available and through hydraulic models where station data were not available. The flood resulted in peak streamflows with recurrence intervals greater than 100 years throughout most of York County, and recurrence intervals up to 50 years in Cumberland County. Peak flows for selected recurrence intervals varied from less than 10 percent to greater than 100 percent different than those in the current FEMA flood-insurance studies due to additional data or newer regression equations. Water-surface elevations observed during the April 2007 flood were bracketed by elevation profiles in FEMA flood-insurance studies with the same recurrence intervals as the recurrence intervals bracketing the observed peak streamflows at seven sites, with higher elevation-profile recurrence intervals than streamflow recurrence intervals at six sites, and with lower elevation-profile recurrence intervals than streamflow recurrence intervals at one site. The April 2007 flood resulted in higher peak flows and water-surface elevations than the flood of May 2006 in coastal locations in York County, and lower peak flows and water-surface elevations than the May 2006 flood further from the coast and in Cumberland County. The Mousam River watershed with over 13 dams and reservoirs was severely impacted by both events. Analyses indicate that the April 2007 peak streamflows in the Mousam River watershed

  16. Plio-Pleistocene Biogenic Opal Deposition in the Southern Ocean

    Science.gov (United States)

    Kuhn, G.; Gersonde, R.

    2002-12-01

    About 2/3 of the annual supply of silicic acid to the World Ocean is buried in the Southern Ocean as biogenic silica (BSi), formed by diatoms and radiolaria in surface waters and exported to the seafloor. Main BSi accumulation occurs in an area between the sea ice edge and the Polar Front Zone and seems to be steered by a complex interaction of biological and physical parameters governing the modern Southern Ocean ecosystem. Sediment cores recovered during Ocean Drilling Program Leg 177 and expeditions with RV POLARSTERN reveal the history of the opal deposition in the Atlantic and Pacific sector of the Southern Ocean during the Pliocene and the Pleistocene. This period is characterized by distinct changes and variability in global climate and ocean circulation that can be related to the spatial-temporal distribution of BSi deposition on long and short time scales. Changes in ocean circulation, water mass structure, sea ice and climatic variability that impact the distribution of silicic acid and the development of coarsly silicified diatoms (e.g. Actinocyclus ingens, Thalassiosira antarctica, Fragilariopsis kerguelensis), presenting the major carriers of biogenic opal, control past BSi deposition in the Southern Ocean. Major deposition in the area of the modern Southern Ocean opal belt starts at the Plio/Pleistocene transition. Such strong export of BSi and related organic carbon might have reinforced the trend of global cooling observed since the Mid-Pliocene climate optimum.

  17. Microbiome of Yermic Regosol in southern Kazakhstan

    Science.gov (United States)

    Kutovaya, Olga; Lebedeva, Marina; Tkhakakhova, Azida

    2014-05-01

    Biological activity is of utmost importance for the genesis of extremely arid desert soils. The soil surface in arid regions is often covered by biofilms representing a complex biocenosis of algae, bacteria, micromycetes, and, sometimes, mosses or lichens. Biofilms of extremely arid soils form a significant part of the living matter in the desert ecosystems and play the central role in their dynamics. Study of the genetic material recovered directly from the soil samples is the main approach in soil metagenomics. Modern sequencing methods were used to describe the diversity of the microorganisms in soil samples. For the first time, such data were obtained for the extremely arid desert soil (Yermic Regosol) in southern Kazakhstan (flat alluvial plain; 43° 42'53.2" N; 79°25'29.1" E; 615 m asl). Taxonomic identification of nucleotide sequences and comparative analysis of microbial communities were performed using VAMPS. The classification of the sequences was performed using RDP. As the primers used were based on the sequences of 16S-rRNA gene of bacteria and archaea, we could analyze the prokaryotic community. Along with bacteria and archaea with established systematic position, all soil samples contained unidentified sequences (5.2-5.3%). Bacteria predominated at the domain level (65.9-74.9%), although their portion was much lower in comparison with that in less arid soils, where it reached 94-100%. Archaea were present as minor components (0.3-0.5%). Dominant groups of bacteria were represented by Proteobacteria (43.9-50.8%), Actinobacteria (9.5-10%), Firmicutes (0.8-2.4%), Verrucomicrobia (1.1-3%), Acidobacteria (1.1-2%), Bacteroidetes (1.2-1.4%). The portion of other phyla was less than 1%. Thus, bacterial phyla Proteobacteria, Actinobacteria and Acidobacteria constitute the core component of the microbiome. Archaea are represented by phylum Crenarchaeota. A key feature of the extremely arid soils is the presence of large numbers (24.7-33.6%) of cyanobacteria

  18. Atmospheric Teleconnections of Northern Hemisphere cooling to the Southern Hemisphere midlatitudes, and implications for Southern Ocean ventilation

    Science.gov (United States)

    Chiang, John; Lee, Shih-Yu; Matsumoto, Katsumi; Tokos, Kathy

    2010-05-01

    Recent marine proxy studies, most notably by Anderson et al. (2009), show intensification of wind-driven upwelling in the Southern Ocean during Heinrich events, and suggesting the possibility of robust atmospheric teleconnections from the Northern Hemisphere affecting the Southern Hemisphere midlatitude westerlies. We explore the latter hypothesis using simulations of an AGCM coupled to a reduced-gravity ocean, and with reference to current thinking regarding extratropical-tropical atmospheric dynamical linkages. When we simulate a Heinrich-like event in our model (by cooling the North Atlantic), we find a significant strengthening of the southern midlatitude westerlies, in particular during the austral winter (JJA), and in the South Pacific. The other pronounced climate change is a marked southward shift of the tropical rainbelt, indicating alteration of the Hadley circulation. Our analysis indicates that the teleconnection can be broken into two parts: first, the northern hemisphere cooling shifting the ITCZ southwards with a pronounced effect on the Hadley circulation (Lindzen and Hou 1988), and then the altered Hadley circulation in turn affecting the southern midlatitude westerlies through the former's control of the southern subtropical westerlies and subsequent effect on the eddy-driven midlatitude westerlies (Lee and Kim, 2003). The seasonal (JJA) and regional (South Pacific) preference of the teleconnection's effects can be explained in terms of the peculiarities of the regional atmospheric dynamics. As an aside, we also find that the growth or decay of the Laurentide ice sheet can also generate this type of north-south teleconnection, although the dynamics are somewhat different. With regards to possible implications for southern ocean ventilation and atmospheric CO2: we applied the wind changes we obtained in our AGCM 'Heinrich' simulation to a global biogeochemical model (the Minnesota Earth System Model for Ocean biogeochemistry), and found a ~20ppm

  19. Simulating Groundwater Recharge Across the Southern High Plains

    Science.gov (United States)

    Smidt, S. J.; Haacker, E. M.; Kendall, A. D.; Hyndman, D. W.

    2015-12-01

    Quantifying recharge and water availability across the Southern High Plains is a difficult, but necessary, challenge for future groundwater and agricultural projections. Overland flow is not common due to limited precipitation, dry soils, and high evapotranspiration. The majority of runoff is temporarily stored in playa lakes, leading to the bulk of recharge across the region occurring in localized infiltration zones beneath these lakes. Despite the importance of regional recharge estimates, limited information exists that integrates complex characteristics of the land, climate, and hydrology in order to quantify recharge across the entire Southern High Plains aquifer. This study applies the Landscape Hydrology Model (LHM) to capture these characteristics and simulate surface water flow and groundwater recharge. This model simulates the complete water cycle across large regions, including irrigation estimates, establishing a framework to estimate recharge and groundwater availability in the Southern High Plains region. Results from this study can be used to predict the likely impacts of climate change and improve water management strategies.

  20. Notes on southern Africa Jerusalem crickets (Orthoptera: Stenopelmatidae: Sia).

    Science.gov (United States)

    Weissman, David B; Bazelet, Corinna S

    2013-02-18

    The Old World Jerusalem cricket (JC) subfamily Siinae contains one genus, Sia, with two subgenera: Sia (Sia) with two fully winged species from southeast Asia, and Sia (Maxentius) with four wingless species from southern Africa. Because there is a dearth of published data about the behavior and biology of these insects, we present new field and laboratory research on southern African Sia (Maxentius), gather museum and literature information, and present guidelines for collecting and rearing specimens. While we make no taxonomic decisions, this review should be useful for future studies, including a needed taxonomic revision. We also compare results from these southern African JCs with recent investigations on related New World taxa, where fascinating biological traits and extensive cryptic biodiversity have been uncovered. DNA analysis reveals that these Old and New World JCs are polyphyletic.

  1. A Critical Approach towards Jade Axes in Southern Scandinavia

    DEFF Research Database (Denmark)

    Sørensen, Lasse

    2013-01-01

    Southern Scandinavian jade axes have been interpreted as items of prestigious exchange illustrating contact with the agrarian societies of Central Europe and reflecting agrarian ideas and ideology. They are therefore important in the discussion concerning the process of neolithisation in Northern...... Europe, but the difficulties in differentiating between Neolithic axes of alpine jade from axes imported from other continents has attracted some criticism. Furthermore, some of the jade axes found in Southern Scandinavian collections originate from private collectors, many of whom had contacts all over...... Europe. The axes lack therefore secure archaeological contexts, and may suggest that they have not been found in Scandinavian soil. The aim of this paper is to maintain a critical approach towards the question of the origin of the jade axes from Southern Scandinavia. However, the many imitations of jade...

  2. Southern Appalachian assessment. Summary report, Report 1 of 5

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1996-07-01

    This final report for the Southern Appalachian Man and the Biosphere Program is comprised of two documents: (1) a brief summary of programs and projects, and (2) a more extensive summary report included as an attachment. The purpose of the program is to promote a sustainable balance between the conservation of biological diversity, compatible economic uses, and cultural values across the Southern Appalachians. Program and project areas addressing regional issues include environmental monitoring and assessment, sustainable development/sustainable technologies, conservation biology, ecosystem management, environmental education and training, cultural and historical resources, and public information and education. The attached summary report is one of five that documents the results of the Southern Appalachian Assessment; it includes atmospheric, social/cultural/economic, terrestrial, and aquatic reports.

  3. Lassa Virus Seroprevalence in Sibirilia Commune, Bougouni District, Southern Mali.

    Science.gov (United States)

    Sogoba, Nafomon; Rosenke, Kyle; Adjemian, Jennifer; Diawara, Sory Ibrahim; Maiga, Ousmane; Keita, Moussa; Konaté, Drissa; Keita, Abdoul Salam; Sissoko, Ibrahim; Boisen, Matt; Nelson, Diana; Oottamasathien, Darin; Millett, Molly; Garry, Robert F; Branco, Luis M; Traoré, Sékou F; Doumbia, Seydou; Feldmann, Heinz; Safronetz, David

    2016-04-01

    Lassa virus (LASV) is endemic to several nations in West Africa. In Mali, LASV was unknown until an exported case of Lassa fever was reported in 2009. Since that time, rodent surveys have found evidence of LASV-infected Mastomys natalensis rats in several communities in southern Mali, near the border with Côte d'Ivoire. Despite increased awareness, to date only a single case of Lassa fever has been confirmed in Mali. We conducted a survey to determine the prevalence of LASV exposure among persons in 3 villages in southern Mali where the presence of infected rodents has been documented. LASV IgG seroprevalence ranged from 14.5% to 44% per village. No sex bias was noted; however, seropositivity rates increased with participant age. These findings confirm human LASV exposure in Mali and suggest that LASV infection/Lassa fever is a potential public health concern in southern Mali.

  4. Assessment of Southern California environment from ERTS-1

    Science.gov (United States)

    Bowden, L. W.; Viellenave, J. H.

    1973-01-01

    ERTS-1 imagery is a useful source of data for evaluation of earth resources in Southern California. The improving quality of ERTS-1 imagery, and our increasing ability to enhance the imagery has resulted in studies of a variety of phenomena in several Southern California environments. These investigations have produced several significant results of varying detail. They include the detection and identification of macro-scale tectonic and vegetational patterns, as well as detailed analysis of urban and agricultural processes. The sequential nature of ERTS-1 imagery has allowed these studies to monitor significant changes in the environment. In addiation, some preliminary work has begun directed toward assessing the impact of expanding recreation, agriculture and urbanization into the fragile desert environment. Refinement of enhancement and mapping techniques and more intensive analysis of ERTS-1 imagery should lead to a greater capability to extract detailed information for more precise evaluations and more accurate monitoring of earth resources in Southern California.

  5. Large-Scale Weather Disturbances in Mars’ Southern Extratropics

    Science.gov (United States)

    Hollingsworth, Jeffery L.; Kahre, Melinda A.

    2015-11-01

    Between late autumn and early spring, Mars’ middle and high latitudes within its atmosphere support strong mean thermal gradients between the tropics and poles. Observations from both the Mars Global Surveyor (MGS) and Mars Reconnaissance Orbiter (MRO) indicate that this strong baroclinicity supports intense, large-scale eastward traveling weather systems (i.e., transient synoptic-period waves). These extratropical weather disturbances are key components of the global circulation. Such wave-like disturbances act as agents in the transport of heat and momentum, and generalized scalar/tracer quantities (e.g., atmospheric dust, water-vapor and ice clouds). The character of large-scale, traveling extratropical synoptic-period disturbances in Mars' southern hemisphere during late winter through early spring is investigated using a moderately high-resolution Mars global climate model (Mars GCM). This Mars GCM imposes interactively lifted and radiatively active dust based on a threshold value of the surface stress. The model exhibits a reasonable "dust cycle" (i.e., globally averaged, a dustier atmosphere during southern spring and summer occurs). Compared to their northern-hemisphere counterparts, southern synoptic-period weather disturbances and accompanying frontal waves have smaller meridional and zonal scales, and are far less intense. Influences of the zonally asymmetric (i.e., east-west varying) topography on southern large-scale weather are examined. Simulations that adapt Mars’ full topography compared to simulations that utilize synthetic topographies emulating key large-scale features of the southern middle latitudes indicate that Mars’ transient barotropic/baroclinic eddies are highly influenced by the great impact basins of this hemisphere (e.g., Argyre and Hellas). The occurrence of a southern storm zone in late winter and early spring appears to be anchored to the western hemisphere via orographic influences from the Tharsis highlands, and the Argyre

  6. Southern images - RGP gmap | LSDB Archive [Life Science Database Archive metadata

    Lifescience Database Archive (English)

    Full Text Available [ Credits ] BLAST Search Image Search Home About Archive Update History Contact us RGP gmap Southern image...s Data detail Data name Southern images Description of data contents Parents Southern hybridization image... files. Data file File name: rgp_gmap_southern_image.zip File URL: ftp://ftp.bioscienced...bc.jp/archive/rgp-gmap/LATEST/rgp_gmap_southern_image.zip File size: 37.3 MB Simp...y of This Database Site Policy | Contact Us Southern images - RGP gmap | LSDB Archive ...

  7. Southern images - RGP gmap98 | LSDB Archive [Life Science Database Archive metadata

    Lifescience Database Archive (English)

    Full Text Available [ Credits ] BLAST Search Image Search Home About Archive Update History Contact us RGP gmap98 Southern image...s Data detail Data name Southern images Description of data contents Parental Southern hybridization image... files. Data file File name: rgp_gmap98_southern_image.zip File URL: ftp://ftp.biosci...encedbc.jp/archive/rgp-gmap98/LATEST/rgp_gmap98_southern_image.zip File size: 69....te History of This Database Site Policy | Contact Us Southern images - RGP gmap98 | LSDB Archive ...

  8. James Dunlop's historical catalogue of southern nebulae and clusters

    Science.gov (United States)

    Cozens, Glen; Walsh, Andrew; Orchiston, Wayne

    2010-03-01

    In 1826 James Dunlop compiled the second ever catalogue of southern star clusters, nebulae and galaxies from Parramatta (NSW, Australia) using a 23-cm reflecting telescope. Initially acclaimed, the catalogue and author were later criticised and condemned by others - including Sir John Herschel and both the catalogue and author are now largely unknown. The criticism of the catalogue centred on the large number of fictitious or ‘missing’ objects, yet detailed analysis reveals the remarkable completeness of the catalogue, despite its inherent errors. We believe that James Dunlop was an important early Australian astronomer, and his catalogue should be esteemed as the southern equivalent of Messier's famous northern catalogue.

  9. A synopsis of the tribe Desmodieae (Fabaceae in southern Africa

    Directory of Open Access Journals (Sweden)

    B. D. Schrire

    1988-12-01

    Full Text Available The tribe Desmodieae has a pantropical distribution and is one of the most advanced tribes in the subfamily Papilionoideae. Its greatest centres of development are in tropical Asia and America. Africa is relatively poorly endowed and only four genera comprising 16 species occur in the flora of southern Africa. Many of these species are widespread in the Old World tropics and the few African endemics appear to be closely related to them. A synopsis of the genera Desmodium, Pseudarthria, Alysicarpus and Lespedeza is given for southern Africa.

  10. Climate change: an issue for parliamentarians in Southern Africa

    Energy Technology Data Exchange (ETDEWEB)

    Chandani, Achala; Anderson, Simon; Schoch, Corinne; Smith, Barry

    2011-11-15

    Parliamentarians can play a key role in building climate resilience by bringing constituents' concerns into national forums, scrutinising how governments are responding to domestic and global climate change issues, and ensuring policy continuity. In the Southern African Customs Union, members of parliament often struggle to fulfill this role, hampered by limited understanding of the issues, fragmented policy and legal frameworks and competing priorities. Boosting parliamentarians' capacity to engage effectively with climate change in Southern Africa requires them to strengthen their research capabilities, diversify their sources of information and build cross-party groups on climate change.

  11. The quinoa boom of the southern Bolivian Altiplano

    DEFF Research Database (Denmark)

    Sander, Lasse; Jacobsen, Sven-Erik

    2014-01-01

    like drought, frost, or soil salinity. Quinoa is thus an exceptional income opportunity in the arid southern Bolivian Altiplano, an area endemically struck by rural poverty and malnutrition. In the early 1970s, the Bolivian government introduced the first tractors to southern Bolivia’s Salar region......, and rapid drainage during precipitation and snow melt events. It appears that many of the first quinoa fields were established on flood plain deposits, where good yields could be anticipated despite the apparent risk of erosion. The subsequent expansion of production areas was paralleled by an increase...

  12. Ecosystem stressors in southern Nevada: Chapter 2 in The Southern Nevada Agency Partnership science and research synthesis: science to support land management in southern Nevada

    Science.gov (United States)

    Pendleton, Burton K.; Chambers, Jeanne C.; Brooks, Matthew L.; Ostoja, Steven M.

    2013-01-01

    Southern Nevada ecosystems and their associated resources are subject to a number of global and regional/local stressors that are affecting the sustainability of the region. Global stressors include elevated carbon dioxide (CO2) concentrations and associated changes in temperature and precipitation patterns and amount, solar radiation, and nutrient cycles (Smith and others 2009b). Global stressors are ubiquitous in nature and interact both directly and indirectly with regional or local stressors. Regional/local stressors in southern Nevada include: population growth and urbanization and associated increases in nitrogen deposition, energy development, water development, and recreation; increased effects of insects and disease; ongoing effects of livestock, wild horse and burro grazing; new and expanding invasive species; and altered fire regimes. This chapter provides background information on the stressors affecting southern Nevada's ecosystems that is needed to address Goal 1.0 in the SNAP Science Research Strategy, which is to restore, sustain, and enhance southern Nevada's ecosystems (Turner and others 2009). Human population growth and changes in land use strongly affect the type and magnitude of local/regional stressors. From 1960 to 2010, Nevada's growth rate was the highest in the nation (www.census.gov/prod/cen2010/briefs/c2010br-01.pdf). Clark County has experienced particularly high growth, with a population increase of greater than 40 percent since the 2000 census. Factors like land ownership, historic and current land use, proximity to human and energy developments, and desirability for recreation all influence the level of human-caused stress. The strong elevation/climate gradients and large difference in the environmental characteristics of southern Nevada ecosystems (fig. 1.2; Chapter 1) have a major influence on both patterns of land use and the dominant stressors for different ecosystem types. Shifts in land use related to population growth

  13. Tidal and inertial currents in the southern Bay of Campeche, southern Gulf of Mexico

    Science.gov (United States)

    Exposito-Diaz, G.

    2007-05-01

    Data from March 1 to June 17, 1997 of currents, water temperature, salinity, and sea level in the southern Bay of Campeche were used to describe tidal and inertial currents. Data were low pass filtered using a Lanczos type filter, and transformed to the frequency space with classical Fast Fourier Transform (FFT), and Morlet Wavelets Method (MWM). Results presented the strongest currents in the southwestern part of the bay, with a dominant east-west component. The predominant direction of the current was parallel to the coast, and followed the bathymetry, depicting the strong barotropic effect. The amplitude of tidal current was very small when compared to total amplitudes. The spectral energy was concentrated at the low frequencies bands, correspond to inertial periods, with an approximate duration between two to three days. Some of these oscillations were also observed in the progressive vectors diagrams. The dominance of the spectral energy at low frequencies demonstrated the influence of the mesoscale cyclonic and the small anticyclonic eddies, of the northeastern Bay of Campeche, in southwestern currents.

  14. The gelechiid fauna of the southern Ural Mountains, part II

    DEFF Research Database (Denmark)

    Junnilainen, Jari; Karsholt, Ole; Nupponen, Kari;

    2010-01-01

    A list comprising 236 species in the family Gelechiidae from the southern Ural Mountains is presented. The material was collected during 1996–2007 on 21 different Finnish-Russian expeditions. The following new combinations are proposed: Caulastrocecis interstratella (Christoph, 1873) comb. n...

  15. Growth and Visual Information Processing in Infants in Southern Ethiopia

    Science.gov (United States)

    Kennedy, Tay; Thomas, David G.; Woltamo, Tesfaye; Abebe, Yewelsew; Hubbs-Tait, Laura; Sykova, Vladimira; Stoecker, Barbara J.; Hambidge, K. Michael

    2008-01-01

    Speed of information processing and recognition memory can be assessed in infants using a visual information processing (VIP) paradigm. In a sample of 100 infants 6-8 months of age from Southern Ethiopia, we assessed relations between growth and VIP. The 69 infants who completed the VIP protocol had a mean weight z score of -1.12 plus or minus…

  16. Coastal zone management around the southern North Sea

    NARCIS (Netherlands)

    Hillen, R.; Van de Wetering, B.G.M.; Verhagen, H.J.

    1993-01-01

    The Southern North Sea is bordered by Great-Britain, France, Belgium, The Netherlands, Germany and Denmark. The North Sea basin and its adjacent shorelines are intensively used. Management of the basin and the coastal zone is therefore essential. Because of the small scale of the area, the dense pop

  17. Linguistic Struggles within and beyond the Southern African Development Community

    Science.gov (United States)

    Bagwasi, Mompoloki Mmangaka

    2012-01-01

    This paper argues that the linguistic struggles faced by the Southern African Development Community (the SADC) represent common linguistic struggles found in Africa and the world where some languages are accused of dominating, stifling and suppressing others. However, the language situation within the SADC is interesting because it offers us a…

  18. Southern Ocean warming delayed by circumpolar upwelling and equatorward transport

    Science.gov (United States)

    Armour, Kyle C.; Marshall, John; Scott, Jeffery R.; Donohoe, Aaron; Newsom, Emily R.

    2016-07-01

    The Southern Ocean has shown little warming over recent decades, in stark contrast to the rapid warming observed in the Arctic. Along the northern flank of the Antarctic Circumpolar Current, however, the upper ocean has warmed substantially. Here we present analyses of oceanographic observations and general circulation model simulations showing that these patterns--of delayed warming south of the Antarctic Circumpolar Current and enhanced warming to the north--are fundamentally shaped by the Southern Ocean's meridional overturning circulation: wind-driven upwelling of unmodified water from depth damps warming around Antarctica; greenhouse gas-induced surface heat uptake is largely balanced by anomalous northward heat transport associated with the equatorward flow of surface waters; and heat is preferentially stored where surface waters are subducted to the north. Further, these processes are primarily due to passive advection of the anomalous warming signal by climatological ocean currents; changes in ocean circulation are secondary. These findings suggest the Southern Ocean responds to greenhouse gas forcing on the centennial, or longer, timescale over which the deep ocean waters that are upwelled to the surface are warmed themselves. It is against this background of gradual warming that multidecadal Southern Ocean temperature trends must be understood.

  19. Molecular epidemiology of canine parvovirus in southern India

    Directory of Open Access Journals (Sweden)

    V. M. Vivek Srinivas,

    2013-08-01

    Full Text Available Aim: The present study was conducted to isolate and characterize canine parvovirus circulating in Southern India by genetic analysis of VP2 capsid protein gene.Materials and Methods: In this study, 128 samples were collected from nine different locations covering five Southern Indian states (Pondicherry, Tamil Nadu, Kerala, Andhra Pradesh and Karnataka . Out of 128 samples, 69 samples were found to be positive by PCR assay. Out of 69 positive samples, 36 were randomly selected and processed for virus isolation. Twenty viruses could be isolated successfully and 18 randomly selected isolate were subjected to VP2 gene sequence analysis along with 6 random clinical samples.Result: Seventeen isolates and 5 clinical samples were characterized as New CPV-2a (CPV2a with 297-Ser→Ala. But one isolate and one clinical sample had amino acids variations which were characteristics of New CPV-2b. The phylogenetic analysis revealed that one of the field isolates was found to be phylogenetically closely related to New CPV-2b strains of India; rest other sequences was found to share ancestral origins with New CPV-2a reference strains of Japan, China, Thailand and India.Conclusion: The present study revealed that the predominant CPV strain circulating in Southern India is New CPV-2a. There is also enough indication of New CPV-2b strain from different states of Southern India.

  20. Equatorial ionospheric electrodynamic perturbations during Southern Hemisphere stratospheric warming events

    DEFF Research Database (Denmark)

    Olson, M. E.; Fejer, B. G.; Stolle, Claudia;

    2013-01-01

    We use ground-based and satellite measurements to examine, for the first time, the characteristics of equatorial electrodynamic perturbations measured during the 2002 major and 2010 minor Southern Hemisphere sudden stratospheric warming (SSW) events. Our data suggest the occurrence of enhanced qu...

  1. Intention for Healthy Eating among Southern Appalachian Teens

    Science.gov (United States)

    Wu, Tiejian; Snider, Jeromy Blake; Floyd, Michael R.; Florence, James E.; Stoots, James Michael; Makamey, Michael I.

    2009-01-01

    Objective: To describe the intention for healthy eating and its correlates among southern Appalachian teens. Methods: Four hundred sixteen adolescents 14 to 16 years old were surveyed with self-administered questionnaires. Results: About 30% of the adolescents surveyed had definite intentions to eat healthfully during the next 2 weeks. The scales…

  2. New taxa of southern African Sesiini (Lepidoptera: Sesiidae).

    Science.gov (United States)

    Bartsch, Daniel

    2015-05-11

    Two new genera of Sesiini from southern Africa, each comprising only a single species are described and figured: Microsesia nana, new gen., new sp. (holotype in TMPS) and Coccophila, new gen., with Coccophila pythes (Druce, 1899), new comb. (holotype in BMNH). Bionomical data for Coccophila pythes are given for the first time.

  3. GAP SIZE AND SUCCESSIONAL PROCESSES IN SOUTHERN APPALACHIAN FORESTS

    Science.gov (United States)

    We used clearcut logging in establishing four replicated sizes of canopy openings (0.016, 0.08, 0.4, and 2.0 ha) in a southern Appalachian hardwood forest in 1981 to examine the long-term effects of disturbance size on plant community structure, biomass accumulation, aboveground ...

  4. Mycobacterium tuberculosis in Wild Asian Elephants, Southern India.

    Science.gov (United States)

    Zachariah, Arun; Pandiyan, Jeganathan; Madhavilatha, G K; Mundayoor, Sathish; Chandramohan, Bathrachalam; Sajesh, P K; Santhosh, Sam; Mikota, Susan K

    2017-03-01

    We tested 3 ild Asian elephants (Elephas maximus) in southern India and confirmed infection in 3 animals with Mycobacterium tuberculosis, an obligate human pathogen, by PCR and genetic sequencing. Our results indicate that tuberculosis may be spilling over from humans (reverse zoonosis) and emerging in wild elephants.

  5. Miscellaneous notes on Southern African Gastropoda Euthyneura (Mollusca)

    NARCIS (Netherlands)

    Bruggen, van A.C.

    1967-01-01

    SUMMARY The following new distribution records of southern African terrestrial molluscs seem to be of particular interest: Pupilla fontana (Krss.), Rachis jejuna (M. & P.), Guppya rumrutiensis (Prest.), Xerocerastus burchelli (Gray) (all four new for Rhodesia), Euonyma damarica Jaeck. (new for Cape

  6. An Argument for an Integrated Approach to Teaching Southern Literature

    Science.gov (United States)

    Ellis, Grace

    1978-01-01

    In addition to such writers as William Faulkner, Flannery O'Connor, Carson McCullers, and Eudora Welty, a good course in modern Southern fiction should include black writers such as Zora Hurston, Nella Larsen, Jean Toomer, Richard Wright, Maya Angelou, and Alice Walker. (MKM)

  7. Andes hantavirus variant in rodents, southern Amazon Basin, Peru.

    Science.gov (United States)

    Razuri, Hugo; Tokarz, Rafal; Ghersi, Bruno M; Salmon-Mulanovich, Gabriela; Guezala, M Claudia; Albujar, Christian; Mendoza, A Patricia; Tinoco, Yeny O; Cruz, Christopher; Silva, Maria; Vasquez, Alicia; Pacheco, Víctor; Ströher, Ute; Guerrero, Lisa Wiggleton; Cannon, Deborah; Nichol, Stuart T; Hirschberg, David L; Lipkin, W Ian; Bausch, Daniel G; Montgomery, Joel M

    2014-02-01

    We investigated hantaviruses in rodents in the southern Amazon Basin of Peru and identified an Andes virus variant from Neacomys spinosus mice. This finding extends the known range of this virus in South America and the range of recognized hantaviruses in Peru. Further studies of the epizoology of hantaviruses in this region are warranted.

  8. A new species of Symplocos (Symplocaceae) from southern Ecuador.

    Science.gov (United States)

    Ulloa Ulloa, Carmen; Ståhl, Bertil; Minga, Danilo; Ansaloni, Raffaella

    2015-01-01

    A new species from Ecuador, Symplocos limonensis, is here described and illustrated. It resembles Symplocos clethrifolia but differs by having larger leaves with evident (i.e., not concealed) areoles on lower surface, sessile inflorescences, smaller white corollas, and fewer stamens. The species is only known from three collections in the Andean forests of Morona-Santiago Province in southern Ecuador.

  9. Livestock First Reached Southern Africa in Two Separate Events.

    Directory of Open Access Journals (Sweden)

    Karim Sadr

    Full Text Available After several decades of research on the subject, we now know when the first livestock reached southern Africa but the question of how they got there remains a contentious topic. Debate centres on whether they were brought with a large migration of Khoe-speakers who originated from East Africa; or whether the livestock were traded down-the-line among hunter-gatherer communities; or indeed whether there was a long history of diverse small scale population movements in this part of the world, one or more of which 'infiltrated' livestock into southern Africa. A new analysis of the distribution of stone toolkits from a sizeable sample of sub-equatorial African Later Stone Age sites, coupled with existing knowledge of the distribution of the earliest livestock remains and ceramics vessels, has allowed us to isolate two separate infiltration events that brought the first livestock into southern Africa just over 2000 years ago; one infiltration was along the Atlantic seaboard and another entered the middle reaches of the Limpopo River Basin. These findings agree well with the latest results of genetic research which together indicate that multiple, small-scale infiltrations probably were responsible for bringing the first livestock into southern Africa.

  10. Towards an effective commons governance system in Southern Africa?

    Directory of Open Access Journals (Sweden)

    Lapologang Magole

    2010-09-01

    Full Text Available This special feature presents several papers generated under the EU-funded ‘Cross Sectoral Commons Governance in Southern Africa’ (CROSCOG project. The feature builds on knowledge generated in case studies which explored existing integrated resource knowledge and governance practices of rural people living in Southern African commons. In earlier generations, especially during the pre-colonial periods, most Southern African societies developed quite effective indigenous institutions for the management of entire landscapes and their component ecosystems, when this was in their interest. Few of these integrated Southern African systems are effective today as they have gone through massive changes, for example due to colonial influences, the increased role of the market and/or conflicts over use and access to natural resources. Meanwhile, most efforts to rebuild or affirm (the management of the commons through various initiatives, have been specific to certain resources or localised areas. Conversely, the smaller number of ecosystem-wide land use planning initiatives that sought to enhance overall environmental health have been dominated by technical, anti-political approaches that failed to understood the differential roles of resources in the spectrum of local livelihoods, and failed to achieve the required broader reinforcement of local governance. This introduction and the papers it introduces explore opportunities and challenges with respect to integrating scale – landscapes, ecosystems, and governing systems – into the local commons.

  11. Procamacolaimus Dorylaimus Sp. Nov. (Nematoda: Leptolaimidae) from the southern atlantic

    NARCIS (Netherlands)

    Holovachov, O.V.

    2003-01-01

    Procamacolaimus dorylaimus sp. nov. is described from the Southern Atlantic (South Shetland Islands, Antarctica). The species is characterised by 3.4-3.5 mm long body; coarsely annulated cuticle without lateral field, epidermal glands and body pores absent, somatic setae present; labial sensilla pap

  12. Brief Analysis of E-business in China Southern Airlines

    Institute of Scientific and Technical Information of China (English)

    刘航; 崔涛; 焦斌

    2007-01-01

    The rapid growth of internet is causing another industrial revolution. Developing E-business is necessary for Chinese airlines to keep their competitive advantages. It is quite urgent for China Southern Airlines to enlarge and improve its E-business strategies so that it can consolidate its leading position in this market segment.

  13. The Southern Tyrrhenian subduction system. Recent evolution and neotectonic implications

    Energy Technology Data Exchange (ETDEWEB)

    Argnani, A. [Consiglio Nazionale delle Ricerche, Bologna (Italy). Ist. per la Geologia Marina

    2000-06-01

    Geological and geophysical data have been integrated with the aim of presenting a new evolutionary model for the Southern Tyrrhenian (Mediterranean Sea) and adjacent regions. The Southern Tyrrhenian back arc basin opened within a plate convergence regime because of sinking slab was torn apart on either side in the last 2 Ma and this process controlled the neotectonic of the Southern Apennines - Tyrrhenian region. On the north-eastern side the slab broke off from NW to SE and this process triggered volcanism and NW-SE extension along the Eastern Tyrrhenian margin, and strike-slip tectonics along NW-SE trending faults in Northern Calabria (Italy). On the south-western side the slab broke off from W to E along the Aeolian Island alignment, although the tear has currently been reoriented along the favouring the NNW-SSE Malta escarpment. During its sinking the subducted slab also detected from the overriding plate, favouring the wedging of the asthenosphere between the two plates and the regional uplift of the Calabrian arc and surroundings. This regional uplift promoted gravitational instability within the orogenic wedge, particularly towards low topography areas; the large-scale sliding of the Calabrian arc towards the Ionian basin can be the Apennines can be related to accommodation of vertical motions within the fold-nd-thrust belt. The pattern of responsible for extensional earthquakes in Calabria and the Southern Apennines, whereas Africa plate convergence can account for compressional earthquakes in Sicily.

  14. Citizen Participation in School Affairs in Two Southern Cities.

    Science.gov (United States)

    Roaden, Arliss L.

    The effectiveness of organizations for citizen participation in school affairs depends on their influence in effecting school policies and decisions, and on their stability for sustained influence. In Atlanta, neither citizen initiated (Southern Leadership Conference) nor school initiated (Committee of 100) system-wide mechanisms have been very…

  15. Self-harm and self-poisoning in southern India

    DEFF Research Database (Denmark)

    Bose, Anuradha; Sandal Sejbaek, Camilla; Suganthy, Pearline

    2009-01-01

    OBJECTIVE: To record cases of suicide and attempted suicide among a population of 108 000 people living in a primarily rural area of southern India, with the aim of guiding policies and strategies to restrict access to poisonous compounds at community level. METHOD: Community-based surveillance...

  16. Toxoplasma gondii infections in sheep in Sicily, southern Italy

    DEFF Research Database (Denmark)

    Vesco, G; Buffolano, W; La Chiusa, S

    2007-01-01

    The aim of the study was to determine the burden of Toxoplasma gondii-infections in sheep in Sicily, southern Italy and the risk factors for infection. Sera from 1961 sheep were collected just before slaughtering from 62 farms located in 8 out of 9 Sicilian administrative districts. The sera were...

  17. Acateco de la Frontera Sur (Acateco of the Southern Border).

    Science.gov (United States)

    Maldonado, Roberto Zavala

    This document is one of 17 volumes on indigenous Mexican languages and is the result of a project undertaken by the Archivo de Lenguas Indigenas de Mexico. This volume contains information on Acatec, an indigenous language of Mexico spoken along Mexico's southern border. The objective of collecting such a representative sampling of the linguistic…

  18. Early Eocene birds from La Borie, southern France

    DEFF Research Database (Denmark)

    Bourdon, Estelle; Mourer-Chauviré, Cécile; Laurent, Yves

    2016-01-01

    The Early Eocene locality of La Borie is located in the village of Saint-Papoul, in southern France. These Eocene fluvio-lacustrine clay deposits have yielded numerous vertebrate remains. Mammalian taxa found in the fossiliferous levels indicate an age near the reference level MP 8–9, which corre...

  19. Challenging School Leadership in Thailand's Southern Border Provinces

    Science.gov (United States)

    Maxcy, Brendan D.; Sungtong, Ekkarin; Nguyen, Thu Su'o'ng Thi

    2010-01-01

    This article reports on the challenges facing public school leadership in southern Thailand, a region destabilized by renewal of a longstanding insurgency. Seeking to implement national educational reforms on shifting social, political and cultural terrain, educators in this region face extraordinary challenges as they reconsider and renegotiate…

  20. A Christian Value? Faculty Diversity at Southern Evangelical Campuses

    Science.gov (United States)

    Smith, Marquita; Mamiseishvili, Ketevan

    2016-01-01

    This case study research project examined efforts at three member institutions of the Council for Christian Colleges & Universities (CCCU), all located in the southern United States, to increase faculty diversity. The study also explored how these efforts related to institutional mission and what aspects within the history of evangelicalism…

  1. Incorporating Phaeocystis into a Southern Ocean ecosystem model

    Science.gov (United States)

    Wang, Shanlin; Moore, J. Keith

    2011-01-01

    Phaeocystis antarctica is an important phytoplankton species in the Southern Ocean. We incorporated P. antarctica into the biogeochemical elemental cycling ocean model to study Southern Ocean ecosystem dynamics and biogeochemistry. The optimum values of ecological parameters for Phaeocystis were sought through synthesizing laboratory and field observations, and the model output was evaluated with observed chlorophyll a, carbon biomass, and nutrient distributions. Several factors have been proposed to control Southern Ocean ecosystem structure, including light adaptation, iron uptake capability, and loss processes. Optimum simulation results were obtained when P. antarctica had a relatively high α (P-I curve initial slope) value and a higher half-saturation constant for iron uptake than other phytoplankton. Simulation results suggested that P. antarctica had a competitive advantage under low irradiance levels, especially in the Ross Sea and Weddell Sea. However, the distributions of P. antarctica and diatoms were also strongly influenced by iron availability. Although grazing rates had an influence on total biomass, our simulations did not show a strong influence of grazing pressure in the competition between P. antarctica and diatoms. However, limited observations and the relative simplicity of zooplankton in our model suggest further research is needed. Overall, P. antarctica contributed ˜13% of annual primary production and ˜19% of sinking carbon export in the Southern Ocean (>40°S) in our best case simulation. At higher latitudes (>60°S) P. antarctica accounts for ˜23% of annual primary production and ˜30% of sinking carbon export.

  2. History of ‘swine fever’ in Southern Africa

    Directory of Open Access Journals (Sweden)

    Mary-Louise Penrith

    2013-02-01

    Full Text Available The histories of the two swine fevers in southern Africa differ widely. Classical swine fever (hog cholera has been known in the northern hemisphere since 1830 and it is probable that early cases of ‘swine fever’ in European settlers’ pigs in southern Africa were accepted to be that disease. It was only in 1921 that the first description of African swine fever as an entity different from classical swine fever was published after the disease had been studied in settlers’ pigs in Kenya. Shortly after that, reports of African swine fever in settlers’ pigs emerged from South Africa and Angola. In South Africa, the report related to pigs in the north-eastern part of the country. Previously (in 1905 or earlier a disease assumed to be classical swine fever caused high mortality among pigs in the Western Cape and was only eradicated in 1918. African swine fever was found over the following years to be endemic in most southern African countries. Classical swine fever, however, apart from an introduction with subsequent endemic establishment in Madagascar and a number of introductions into Mauritius, the last one in 2000, had apparently remained absent from the region until it was diagnosed in the Western and subsequently the Eastern Cape of South Africa in 2005. It was eradicated by 2007. The history of these diseases in the southern African region demonstrates their importance and their potential for spread over long distances, emphasising the need for improved management of both diseases wherever they occur.

  3. A new species of Fernandezina (Araneae, Palpimanidae from southern Brazil

    Directory of Open Access Journals (Sweden)

    Ricardo Ott

    2014-12-01

    Full Text Available A new species of Fernandezina Birabén, 1951, F. nica sp. nov. is described from Rio Grande do Sul state, in southern Brazil. Fernandezina pulchra Birabén, 1951, is registered for Brazil and a new geographic record in Brazil is presented for F. pelta Platnick, 1975.

  4. Marketing in Southern Ghana: towards a planning typology

    NARCIS (Netherlands)

    D.M. Dunham (David)

    1974-01-01

    textabstractThere are a number of strong historical and practical grounds for a regional planner operating in Southern Ghana to study marketing structures. The recent history of the area confirms an important axiom of regional theory, namely that the organisation of marketing has a considerable infl

  5. 75 FR 77677 - Southern Nuclear Operating Company, Inc.

    Science.gov (United States)

    2010-12-13

    ... Background The Southern Nuclear Operating Company, Inc. (SNC, the licensee) is the holder of the Renewed... Exemptions'', SNC has, by letter dated May 12, 2010 (the application), requested an exemption from the fuel.... In Section V of the application, SNC provides a technical basis supporting the...

  6. Environmental Education Research in Southern Africa: Dilemmas of Interpretation

    Science.gov (United States)

    Gough, Annette; Gough, Noel

    2004-01-01

    These multiple framings of our reflections on environmental education research in southern Africa are written as dilemmas of interpretation that aim to disrupt any temptation to generalise or essentialise its qualities and characteristics. Recognising that research is a textual practice, we use J. M. Coetzee's portrayal of the dilemmas faced by…

  7. Early Eocene birds from La Borie, southern France

    DEFF Research Database (Denmark)

    Bourdon, Estelle; Mourer-Chauviré, Cécile; Laurent, Yves

    2016-01-01

    The Early Eocene locality of La Borie is located in the village of Saint-Papoul, in southern France. These Eocene fluvio-lacustrine clay deposits have yielded numerous vertebrate remains. Mammalian taxa found in the fossiliferous levels indicate an age near the reference level MP 8–9, which...

  8. A lateral cephalometric study of sella turcica in Southern Chinese

    OpenAIRE

    Mohamed Makhbuol, MZB; Wong, RWK; Rabie, ABM

    2012-01-01

    The dimensions of sella might be associated with pathological conditions, yet data related to these are scanty. This study is to quantitatively investigate the dimensions of sella in southern Chinese population. This retrospective pilot study consists 150 randomly selected Chinese lateral cephalometric radiographs (50 females and 100 males) presented to PPDH for Orthod...

  9. Preparing for a "Big One": The great southern California shakeout

    Science.gov (United States)

    Jones, L.M.; Benthien, M.

    2011-01-01

    The Great Southern California ShakeOut was a week of special events featuring the largest earthquake drill in United States history. On November 13, 2008, over 5 million Southern Californians pretended that the magnitude-7.8 ShakeOut scenario earthquake was occurring and practiced actions derived from results of the ShakeOut Scenario, to reduce the impact of a real, San Andreas Fault event. The communications campaign was based on four principles: 1) consistent messaging from multiple sources; 2) visual reinforcement: 3) encouragement of "milling"; and 4) focus on concrete actions. The goals of the Shake-Out established in Spring 2008 were: 1) to register 5 million people to participate in the drill; 2) to change the culture of earthquake preparedness in Southern California; and 3) to reduce earthquake losses in Southern California. Over 90% of the registrants surveyed the next year reported improvement in earthquake preparedness at their organization as a result of the ShakeOut. ?? 2011, Earthquake Engineering Research Institute.

  10. "Democratic" Collaboration for School Turnaround in Southern Arizona

    Science.gov (United States)

    Bennett, Jeffrey V.

    2012-01-01

    Purpose: The purpose of this study is to examine the democratic nature of collaboration and related principal influences in one successful Southern Arizona elementary school in a changing demographic and border context in the US Southwest and where the principal did not share the same ethnic identity/cultural background.…

  11. 77 FR 50712 - Information Collection: Southern Alaska Sharing Network and Subsistence Study; Proposed...

    Science.gov (United States)

    2012-08-22

    ... local sharing networks that structure contemporary subsistence-cash economies using research methods... Bureau of Ocean Energy Management Information Collection: Southern Alaska Sharing Network and Subsistence... in Alaska, ``Southern Alaska Sharing Network and Subsistence Study.'' DATES: Submit written...

  12. 75 FR 61835 - Jackson & Lansing Railroad Company-Trackage Rights Exemption-Norfolk Southern Railway Company

    Science.gov (United States)

    2010-10-06

    ... Surface Transportation Board Jackson & Lansing Railroad Company--Trackage Rights Exemption-- Norfolk Southern Railway Company Pursuant to a written Assignment of Trackage Rights and Other Joint Facility Agreements (Trackage Agreement) dated September 16, 2010, Norfolk Southern Railway Company (NSR) has...

  13. Nuances and nuisances : crop production intensification options for smallholder farming systems of southern Africa

    NARCIS (Netherlands)

    Rusinamhodzi, L.

    2013-01-01

    Key words: crop production, intensification, extensification, farming systems, tradeoff analysis, maize, legume, manure, fertiliser, southern Africa Soil fertility decline and erratic rainfall are major constraints to crop productivity on smallholder farms in southern Africa. Crop

  14. SRKW seasonal occurence - Patterns of seasonal occurrence of Southern Resident Killer Whales

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Patterns of seasonal occurrence of Southern Resident Killer Whales (SRKW) throughout their range. Southern Resident Killer Whales are listed as a Distinct Population...

  15. Hemoglobinopathy: molecular epidemiological characteristics and health effects on Hakka people in the Meizhou region, southern China.

    Directory of Open Access Journals (Sweden)

    Min Lin

    Full Text Available BACKGROUND: Hemoglobinopathies are the most common inherited diseases in southern China. However, there have been only a few epidemiological studies of hemoglobinopathies in Guangdong province. MATERIALS AND METHODS: Peripheral blood samples were collected from 15299 "healthy" unrelated subjects of dominantly ethnic Hakka in the Meizhou region, on which hemoglobin electrophoresis and routine blood tests were performed. Suspected cases with hemoglobin variants and hereditary persistence of fetal hemoglobin (HPFH were further characterized by PCR, DNA sequencing, reverse dot blot (RDB or multiplex ligation-dependent probe amplification (MLPA. In addition, 1743 samples were randomly selected from the 15299 subjects for thalassemia screening, and suspected thalassemia carriers were identified by PCR and RDB. RESULTS: The gene frequency of hemoglobin variants was 0.477% (73/15299. The five main subgroups of the ten hemoglobin variants were Hb E, Hb G-Chinese, Hb Q-Tahiland, Hb New York and Hb J-Bangkok. 277 cases (15.89%, 277/1743 of suspected thalassemia carriers with microcytosis (MCV<82 fl were found by thalassemia screening, and were tested by a RDB gene chip to reveal a total of 196 mutant chromosomes: including 124 α-thalassemia mutant chromosomes and 72 β-thalassemia mutant chromosomes. These results give a heterozygote frequency of 11.24% for common α and β thalassemia in the Hakka population in the Meizhou region. 3 cases of HPFH/δβ-thalassemia were found, including 2 cases of Vietnamese HPFH (FPFH-7 and a rare Belgian( Gγ((Aγδβ⁰-thalassemia identified in Chinese. CONCLUSIONS: Our results provide a detailed prevalence and molecular characterization of hemoglobinopathies in Hakka people of the Meizhou region. The estimated numbers of pregnancies each year in the Meizhou region, in which the fetus would be at risk for β thalassemia major or intermedia, Bart's hydrops fetalis, and Hb H disease, are 25 (95% CI, 15 to 38, 40 (95% CI

  16. Insecticide resistance status in Anopheles gambiae in southern Benin

    Directory of Open Access Journals (Sweden)

    Corbel Vincent

    2010-03-01

    Full Text Available Abstract Background The emergence of pyrethroid resistance in Anopheles gambiae has become a serious concern to the future success of malaria control. In Benin, the National Malaria Control Programme has recently planned to scaling up long-lasting insecticidal nets (LLINs and indoor residual spraying (IRS for malaria prevention. It is, therefore, crucial to monitor the level and type of insecticide resistance in An. gambiae, particularly in southern Benin where reduced efficacy of insecticide-treated nets (ITNs and IRS has previously been reported. Methods The protocol was based on mosquito collection during both dry and rainy seasons across forty districts selected in southern Benin. Bioassay were performed on adults collected from the field to assess the susceptibility of malaria vectors to insecticide-impregnated papers (permethrin 0.75%, delthamethrin 0.05%, DDT 4%, and bendiocarb 0.1% following WHOPES guidelines. The species within An. gambiae complex, molecular form and presence of kdr and ace-1 mutations were determined by PCR. Results Strong resistance to permethrin and DDT was found in An. gambiae populations from southern Benin, except in Aglangandan where mosquitoes were fully susceptible (mortality 100% to all insecticides tested. PCR showed the presence of two sub-species of An. gambiae, namely An. gambiae s.s, and Anopheles melas, with a predominance for An. gambiae s.s (98%. The molecular M form of An. gambiae was predominant in southern Benin (97%. The kdr mutation was detected in all districts at various frequency (1% to 95% whereas the Ace-1 mutation was found at a very low frequency (≤ 5%. Conclusion This study showed a widespread resistance to permethrin in An. gambiae populations from southern Benin, with a significant increase of kdr frequency compared to what was observed previously in Benin. The low frequency of Ace-1 recorded in all populations is encouraging for the use of bendiocarb as an alternative insecticide to

  17. The Southern Tyrrhenian subduction system: recent evolution and neotectonic implications

    Directory of Open Access Journals (Sweden)

    A. Argnani

    2000-06-01

    Full Text Available Geological and geophysical data have been integrated with the aim of presenting a new evolutionary model for the Southern Tyrrhenian and adjacent regions. The Southern Tyrrhenian backarc basin opened within a plate convergence regime because of sinking and rollback of the oceanic Ionian lithosphere. On the basis of seismological observations, I infer that the sinking slab was torn apart on either side in the last 2 Ma and this process controlled the neotectonics of the Southern Apennines - Tyrrhenian region. On the north-eastern side the slab broke off from NW to SE and this process triggered volcanism and NW-SE extension along the Eastern Tyrrhenian margin, and strike-slip tectonics along NW-SE trending faults in Northern Calabria. On the south-western side the slab broke off from W to E along the Aeolian Island alignment, although the tear has currently been reoriented along the NNW-SSE Malta escarpment. During its sinking the subducted slab also detached from the overriding plate, favouring the wedging of the asthenosphere between the two plates and the regional uplift of the Calabrian arc and surroundings. This regional uplift promoted gravitational instability within the orogenic wedge, particularly towards low topography areas; the large-scale sliding of the Calabrian arc towards the Ionian basin can be the cause of CW rotation and graben formation in Calabria. Also the E-dipping extensional faults of the Southern Apennines can be related to accommodation of vertical motions within the fold-and-thrust belt. The pattern of recent seismicity reflects this neotectonics where crustal-scale gravity deformation within the orogenic wedge is responsible for extensional earthquakes in Calabria and the Southern Apennines, whereas Africa plate convergence can account for compressional earthquakes in Sicily.

  18. Bioremediation of Southern Mediterranean oil polluted sites comes of age.

    Science.gov (United States)

    Daffonchio, Daniele; Ferrer, Manuel; Mapelli, Francesca; Cherif, Ameur; Lafraya, Alvaro; Malkawi, Hanan I; Yakimov, Michail M; Abdel-Fattah, Yasser R; Blaghen, Mohamed; Golyshin, Peter N; Kalogerakis, Nicolas; Boon, Nico; Magagnini, Mirko; Fava, Fabio

    2013-09-25

    Mediterranean Sea is facing a very high risk of oil pollution due to the high number of oil extractive and refining sites along the basin coasts, and the intense maritime traffic of oil tankers. All the Mediterranean countries have adopted severe regulations for minimizing pollution events and bioremediation feasibility studies for the most urgent polluted sites are undergoing. However, the analysis of the scientific studies applying modern 'meta-omics' technologies that have been performed on marine oil pollution worldwide showed that the Southern Mediterranean side has been neglected by the international research. Most of the studies in the Mediterranean Sea have been done in polluted sites of the Northern side of the basin. Those of the Southern side are poorly studied, despite many of the Southern countries being major oil producers and exporters. The recently EU-funded research project ULIXES has as a major objective to increase the knowledge of the bioremediation potential of sites from the Southern Mediterranean countries. ULIXES is targeting four major polluted sites on the coastlines of Egypt, Jordan, Morocco and Tunisia, including seashore sands, lagoons, and oil refinery polluted sediments. The research is designed to unravel, categorize, catalogue, exploit and manage the diversity and ecology of microorganisms thriving in these polluted sites. Isolation of novel hydrocarbon degrading microbes and a series of state of the art 'meta-omics' technologies are the baseline tools for improving our knowledge on biodegradation capacities mediated by microbes under different environmental settings and for designing novel site-tailored bioremediation approaches. A network of twelve European and Southern Mediterranean partners is cooperating for plugging the existing gap of knowledge for the development of novel bioremediation processes targeting such poorly investigated polluted sites.

  19. 40 CFR 81.99 - New Mexico Southern Border Intrastate Air Quality Control Region.

    Science.gov (United States)

    2010-07-01

    ... Designation of Air Quality Control Regions § 81.99 New Mexico Southern Border Intrastate Air Quality Control Region. The Arizona-New Mexico Southern Border Interstate Air Quality Control Region has been renamed the New Mexico Southern Border Intrastate Air Quality Control Region and has been revised to consist...

  20. 76 FR 18216 - Southern Natural Gas Company; Notice of Request Under Blanket Authorization

    Science.gov (United States)

    2011-04-01

    ... Federal Energy Regulatory Commission Southern Natural Gas Company; Notice of Request Under Blanket Authorization Take notice that on March 16, 2011, Southern Natural Gas Company (Southern), Post Office Box 2563... and 157.216 of the Commission's Regulations under the Natural Gas Act (NGA) as amended, to abandon...

  1. 40 CFR 81.156 - Southern Maryland Intrastate Air Quality Control Region.

    Science.gov (United States)

    2010-07-01

    ... Air Quality Control Regions § 81.156 Southern Maryland Intrastate Air Quality Control Region. The Southern Maryland Intrastate Air Quality Control Region consists of the territorial area encompassed by the... 40 Protection of Environment 17 2010-07-01 2010-07-01 false Southern Maryland Intrastate...

  2. 40 CFR 81.158 - Southern Wisconsin Intrastate Air Quality Control Region.

    Science.gov (United States)

    2010-07-01

    ... Air Quality Control Regions § 81.158 Southern Wisconsin Intrastate Air Quality Control Region. The Southern Wisconsin Intrastate Air Quality Control Region consists of the territorial area encompassed by... 40 Protection of Environment 17 2010-07-01 2010-07-01 false Southern Wisconsin Intrastate...

  3. 77 FR 24483 - Southern Natural Gas Company, L.L.C.; Notice of Filing

    Science.gov (United States)

    2012-04-24

    ... Federal Energy Regulatory Commission Southern Natural Gas Company, L.L.C.; Notice of Filing Take notice that on April 5, 2012, Southern Natural Gas Company, L.L.C. (Southern), 569 Brookwood Village, Suite 501, Birmingham, Alabama 35209, filed an application, pursuant to Section 7(b) of the Natural Gas...

  4. 75 FR 53688 - Southern LNG Company, L.L.C.; Notice of Technical Conference

    Science.gov (United States)

    2010-09-01

    ...] Southern LNG Company, L.L.C.; Notice of Technical Conference August 25, 2010. Take notice that Commission... Commission, 888 First Street, NE., Washington, DC 20426. On June 7, 2010, Southern LNG Company, L.L.C. (Southern LNG) filed a tariff sheet to revise its tariff with respect to gas quality and...

  5. 75 FR 51989 - Southern LNG Company, L.L.C.; Notice of Application

    Science.gov (United States)

    2010-08-24

    ... Energy Regulatory Commission Southern LNG Company, L.L.C.; Notice of Application August 16, 2010. Take notice that on August 4, 2010, Southern LNG Company, L.L.C. (Southern LNG), Post Office Box 2563... with certain facilities located at its liquefied natural gas (LNG) import terminal on Elba Island,...

  6. 77 FR 41975 - Southern Star Central Gas Pipeline, Inc.; Notice of Application

    Science.gov (United States)

    2012-07-17

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF ENERGY Federal Energy Regulatory Commission Southern Star Central Gas Pipeline, Inc.; Notice of Application Take notice that on June 27, 2012, Southern Star Central Gas Pipeline, Inc. (Southern Star), 4700 Highway 56, Owensboro, Kentucky 42301, filed...

  7. 77 FR 38622 - Southern Star Central Gas Pipeline, Inc.; Notice of Request Under Blanket Authorization

    Science.gov (United States)

    2012-06-28

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF ENERGY Federal Energy Regulatory Commission Southern Star Central Gas Pipeline, Inc.; Notice of Request Under Blanket Authorization Take notice that on June 4, 2012, Southern Star Central Gas Pipeline, Inc. (Southern Star), 4700 Highway 56, Owensboro,...

  8. 78 FR 68835 - Southern Star Central Gas Pipeline, Inc.; Notice of Request Under Blanket Authorization

    Science.gov (United States)

    2013-11-15

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF ENERGY Federal Energy Regulatory Commission Southern Star Central Gas Pipeline, Inc.; Notice of Request Under Blanket Authorization Take notice that on October 31, 2013, Southern Star Central Gas Pipeline, Inc. (Southern Star), 4700 Highway 56, Owensboro,...

  9. 78 FR 25264 - Southern Star Central Gas Pipeline, Inc.; Notice of Request Under Blanket Authorization

    Science.gov (United States)

    2013-04-30

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF ENERGY Federal Energy Regulatory Commission Southern Star Central Gas Pipeline, Inc.; Notice of Request Under Blanket Authorization Take notice that on April 16, 2013, Southern Star Central Gas Pipeline, Inc. (Southern Star), 4700 Highway 56, Owensboro,...

  10. 78 FR 53746 - Southern Star Central Gas Pipeline, Inc.; Notice of Request Under Blanket Authorization

    Science.gov (United States)

    2013-08-30

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF ENERGY Federal Energy Regulatory Commission Southern Star Central Gas Pipeline, Inc.; Notice of Request Under Blanket Authorization Take notice that on August 13, 2013, Southern Star Central Gas Pipeline, Inc. (Southern Star), 4700 Highway 56, Owensboro,...

  11. 77 FR 14517 - Southern Star Central Gas Pipeline, Inc.; Notice of Request Under Blanket Authorization

    Science.gov (United States)

    2012-03-12

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF ENERGY Federal Energy Regulatory Commission Southern Star Central Gas Pipeline, Inc.; Notice of Request Under Blanket Authorization Take notice that on February 21, 2012 Southern Star Central Gas Pipeline, Inc. (Southern Star), 4700 State Highway 56,...

  12. 78 FR 13663 - Southern Star Central Gas Pipeline, Inc.; Notice of Request Under Blanket Authorization

    Science.gov (United States)

    2013-02-28

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF ENERGY Federal Energy Regulatory Commission Southern Star Central Gas Pipeline, Inc.; Notice of Request Under Blanket Authorization Take notice that on February 11, 2013, Southern Star Central Gas Pipeline, Inc. (Southern Star), 4700 Highway 56, P.O. Box...

  13. 75 FR 8053 - Southern Star Central Gas Pipeline, Inc.; Notice of Request Under Blanket Authorization

    Science.gov (United States)

    2010-02-23

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF ENERGY Federal Energy Regulatory Commission Southern Star Central Gas Pipeline, Inc.; Notice of Request Under Blanket Authorization February 16, 2010. Take notice that on January 29, 2010, Southern Star Central Gas Pipeline, Inc. (Southern Star), 4700...

  14. SOTER-based soil parameter estimates for Southern Africa (ver. 1.0)

    NARCIS (Netherlands)

    Batjes, N.H.

    2014-01-01

    This harmonized set of soil parameter estimates for Southern Africa has been derived from the 1:2M scale Soil and Terrain Database for Southern Africa (SOTERSAF ver. 1.0) and ISRIC-WISE soil profile database. The land surface of Southern Africa has been characterized using 4022 unique SOTER units, c

  15. Exhumation history of the southern Altiplano plateau (southern Bolivia) constrained by apatite fission track thermochronology

    Science.gov (United States)

    Ege, H.; Sobel, E. R.; Scheuber, E.; Jacobshagen, V.

    2007-02-01

    Although the structural geometry of the Cenozoic Altiplano-Puna plateau in the central Andes is well defined, the temporal evolution of this contractile deformation is poorly constrained. To address this shortcoming, we used apatite fission track thermochronology (AFT) to quantify the cooling and exhumation history along a transect at 21°S in southern Bolivia, through the deformed intermontane Altiplano basin, the doubly vergent thrust belt of the Eastern Cordillera and the inner foreland thrust belt east of the plateau (Interandean Zone). Thermal history modeling combined with published balanced cross sections and stratigraphic data constrain exhumation histories. Exhumation started during the late Eocene (40-36 Ma) in the central Eastern Cordillera, possibly due to bivergent thrusting and Cretaceous rift structure inversion. During the early Oligocene (33-27 Ma), exhumation spread across the study area as the current boundary thrusts of the Eastern Cordillera were activated. The inner west vergent thrust system became active in irregular order until circa 20 Ma, whereas the east vergent Interandean thrust belt formed by eastward propagating deformation since circa 30 Ma. Plateau exhumation continued at ˜0.2 mm/yr until shortening terminated by 11-7 Ma. Shortening within the plateau since circa 30 Ma did not evolve by lateral accretion of thrust wedges; the propagating deformation style is spatially confined to the foreland thrust belt, which initiated coeval to plateau deformation (Interandean Zone) but propagated mainly after circa 10 Ma (Subandean Zone). Early Oligocene plateau-wide tectonically driven exhumation suggests that subduction-related processes had already thermally weakened the continental lithosphere prior to the 27-25 Ma onset of volcanic activity.

  16. Crustal Seismicity and Recent Faults in Southern Peru

    Science.gov (United States)

    David, C.; Comte, D.; Tavera, H.; Audin, L.; Herail, G.

    2004-12-01

    Most seismological studies in southern Peru have been focused on the downgoing slab seismicity in order to constrain the Wadati-Benioff zone. This study deals with the intra-continental seismicity of the southern Peru forearc (17,3° S - 18,5° S) in a post-seismic context (Arequipa thrust earthquake, Mw=8.4, 23 June, 2001). It is difficult to identify historical crustal earthquake from available catalogues, however some crustal events teleseismically recorded can be found since 1976; they exhibit normal focal mechanism solutions in the southern Peru volcanic arc and inverse focal mechanism solutions in the Central Depression. Following a notable increase of shallow crustal seismicity located close to the Western Cordillera after the 23 June 2001, a temporary seismic network was deployed between January and March 2003 in order to study the Wadati-Benioff zone and monitoring the crustal seismicity in southern Peru. From the about 1700 events locally recorded by the local network, 300 crustal earthquakes were identified in the Peruvian forearc between Tacna and Moquegua. This crustal seismicity is distributed along a lineament located at depths between 0 and 60 km, dipping at about 45° from the Western Cordillera towards the coast, almost perpendicular to the subducting slab; this behaviour was previously observed in northern Chile and in southern Peru, north of the study zone (16° S). In the Central Depression, seismic activity is not superficial occurring between 25-60 km depth and it is mostly characterized by inverse focal mechanism solutions. Superficial faults situated in the Central Depression and in the Coastal Cordillera can not be associated with the seismic activity observed in this area. However, in the Pre-Cordillera, crustal seismicity occurs at depths between 0-15 km and can be correlated with shallow fault systems recognized by satellite images and on the field. For examples, the Incapuquio fault system which was a transpressive system in Cretaceous

  17. The Southern Proper Motion Program IV. The SPM4 Catalog

    CERN Document Server

    Girard, T M; Zacharias, N; Vieira, K; Casetti-Dinescu, D I; Monet, D G; Lopez, C E

    2011-01-01

    We present the fourth installment of the Yale/San Juan Southern Proper Motion Catalog, SPM4. The SPM4 contains absolute proper motions, celestial coordinates, and (B,V) photometry for over 103 million stars and galaxies between the south celestial pole and -20 deg declination. The catalog is roughly complete to V=17.5 and is based on photographic and CCD observations taken with the Yale Southern Observatory's double-astrograph at Cesco Observatory in El Leoncito, Argentina. The proper-motion precision, for well-measured stars, is estimated to be 2 to 3 mas/yr, depending on the type of second-epoch material. At the bright end, proper motions are on the International Celestial Reference System by way of Hipparcos Catalog stars, while the faint end is anchored to the inertial system using external galaxies. Systematic uncertainties in the absolute proper motions are on the order of 1 mas/yr.

  18. Will krill fare well under Southern Ocean acidification?

    Science.gov (United States)

    Kawaguchi, So; Kurihara, Haruko; King, Robert; Hale, Lillian; Berli, Thomas; Robinson, James P; Ishida, Akio; Wakita, Masahide; Virtue, Patti; Nicol, Stephen; Ishimatsu, Atsushi

    2011-04-23

    Antarctic krill embryos and larvae were experimentally exposed to 380 (control), 1000 and 2000 µatm pCO₂ in order to assess the possible impact of ocean acidification on early development of krill. No significant effects were detected on embryonic development or larval behaviour at 1000 µatm pCO₂; however, at 2000 µatm pCO₂ development was disrupted before gastrulation in 90 per cent of embryos, and no larvae hatched successfully. Our model projections demonstrated that Southern Ocean sea water pCO₂ could rise up to 1400 µatm in krill's depth range under the IPCC IS92a scenario by the year 2100 (atmospheric pCO₂ 788 µatm). These results point out the urgent need for understanding the pCO₂-response relationship for krill developmental and later stages, in order to predict the possible fate of this key species in the Southern Ocean.

  19. Human Leukocyte Antigen Diversity: A Southern African Perspective

    Directory of Open Access Journals (Sweden)

    Mqondisi Tshabalala

    2015-01-01

    Full Text Available Despite the increasingly well-documented evidence of high genetic, ethnic, and linguistic diversity amongst African populations, there is limited data on human leukocyte antigen (HLA diversity in these populations. HLA is part of the host defense mechanism mediated through antigen presentation to effector cells of the immune system. With the high disease burden in southern Africa, HLA diversity data is increasingly important in the design of population-specific vaccines and the improvement of transplantation therapeutic interventions. This review highlights the paucity of HLA diversity data amongst southern African populations and defines a need for information of this kind. This information will support disease association studies, provide guidance in vaccine design, and improve transplantation outcomes.

  20. New Records of Aedes aegypti In Southern Oklahoma, 2016.

    Science.gov (United States)

    Bradt, David L; Bradley, Kristy K; Hoback, W Wyatt; Noden, Bruce H

    2017-03-01

    Aedes aegypti is an important subtropical vector species and is predicted to have a limited year-round distribution in the southern United States. Collection of the species has not been officially verified in Oklahoma since 1940. Adult mosquitoes were collected in 42 sites across 7 different cities in Oklahoma using 3 different mosquito traps between May and September 2016. Between July and September 2016, 88 Ae. aegypti adults were collected at 18 different sites in 4 different cities across southern Oklahoma. Centers for Disease Control and Prevention mini light traps baited with CO2 attracted the highest numbers of Ae. aegypti individuals compared to Biogents (BG)-Sentinel(®) traps baited with Biogents (BG)-lure and octenol and Centers for Disease Control and Prevention gravid traps baited with Bermuda grass-infused water. The discovery of Ae. aegypti mosquitoes within urban/exurban areas in Oklahoma is important from an ecological as well as a public health perspective.

  1. The quinoa boom of the southern Bolivian Altiplano

    DEFF Research Database (Denmark)

    Sander, Lasse; Jacobsen, Sven-Erik

    2014-01-01

    Quinoa (Chenopodium quinoa Willd.) is a native Andean crop that gained worldwide popularity over the last few decades due to its outstanding nutritional properties. The plant is characterized by an exceptional adaptation and is able to produce decent yields despite harsh environmental conditions...... like drought, frost, or soil salinity. Quinoa is thus an exceptional income opportunity in the arid southern Bolivian Altiplano, an area endemically struck by rural poverty and malnutrition. In the early 1970s, the Bolivian government introduced the first tractors to southern Bolivia’s Salar region...... with the intention to push agricultural development – with obvious success. The cultivation of quinoa is today the most important land use, with a continued increase in production volume and areal extent. We here trace back land-use changes from 1972 to 2013 in one of the most important areas of quinoa production...

  2. Birth of two volcanic islands in the southern Red Sea

    KAUST Repository

    Xu, Wenbin

    2015-05-26

    Submarine eruptions that lead to the formation of new volcanic islands are rare and far from being fully understood; only a few such eruptions have been witnessed since Surtsey Island emerged to the south of Iceland in the 1960s. Here we report on two new volcanic islands that were formed in the Zubair archipelago of the southern Red Sea in 2011–2013. Using high-resolution optical satellite images, we find that the new islands grew rapidly during their initial eruptive phases and that coastal erosion significantly modified their shapes within months. Satellite radar data indicate that two north–south-oriented dykes, much longer than the small islands might suggest, fed the eruptions. These events occurred contemporaneously with several local earthquake swarms of the type that typically accompany magma intrusions. Earthquake activity has been affecting the southern Red Sea for decades, suggesting the presence of a magmatically active zone that has previously escaped notice.

  3. Multi-element geochemical mapping in Southern China.

    Science.gov (United States)

    Cheng, Zhizhong; Xie, Xuejing; Yao, Wensheng; Feng, Jizhou; Zhang, Qin; Fang, Jindong

    2014-04-01

    The 76-element Geochemical Mapping (76 GEM) Project was undertaken in southwestern China in 2000 and in southeastern China in 2008. In this project, 5244 composite samples of stream sediment at a density of one composite sample for each 1:50,000-scale map sheet were prepared from sample archives of the China Regional Geochemistry-National Reconnaissance (RGNR) Project, which have been available since 1978. The 76 elements were analyzed by using inductively coupled plasma mass spectrometry (ICP-MS), X-ray fluorescence (XRF), and inductively coupled plasma atomic emission spectroscopy (ICP-AES). In the present study, a new quality-control method known as the visualized standard map method was applied to the results of the 76 GEM project. Mean value and background value, which indicate the average concentration of the 76 elements in southern China, were derived from statistical data. Moreover, geochemical maps were compiled to demonstrate the distribution of the 76 elements in southern China.

  4. Modulation of the Southern Hemisphere climate by solar radiation management

    Science.gov (United States)

    Phipps, Steven; Lenton, Andrew; Rotstayn, Leon; Gupta, Alex Sen; Ji, Duoying; Moore, John; Niemeier, Ulrike; Schmidt, Hauke; Tilmes, Simone

    2015-04-01

    Geoengineering is increasingly being considered as a means to lessen the climatic impacts of anthropogenic greenhouse gas emissions. However, it is not without significant risks of its own. In this study, we investigate the response of the Southern Hemisphere (SH) climate to solar radiation management (SRM) using Geoengineering Model Intercomparison Project experiments G3 and G3solar. We find that the response to SRM is characterized by a contraction of the Hadley Cell and subtropical dry zones. This is accompanied by a shift towards a less positive state of the Southern Annular Mode and a northward shift of the SH westerly winds, mitigating the trends under projected future anthropogenic forcing. These changes result in an increase in precipitation minus evaporation in the SH subtropics, suggesting that SRM may be effective at counteracting the anthropogenically-driven drying trend in this region. However, any beneficial impacts cease abruptly as soon as geoengineering is terminated.

  5. On the "hidden" phytoplankton blooms on Australia's southern shelves

    Science.gov (United States)

    Kämpf, Jochen; Kavi, Ankit

    2017-02-01

    Phytoplankton blooms on Australia's southern shelves are revisited using satellite-derived monthly data of chlorophyll a concentrations for the period 2003-2015. It is known that the region hosts a seasonal coastal upwelling system that develops in austral summer (January-March) with chlorophyll a concentrations of >2 mg/m3. While this summer upwelling is spatially limited to a few hot spots, here we show that widespread phytoplankton blooms of moderate ( 1 mg/m3) chlorophyll a concentrations develop during autumn and early winter on most of Australia's extensive southern shelves—from the vast shelves of the Great Australian Bight (GAB) in the west to Bass Strait in the east. This surprising finding disproves the widespread belief that shelf waters of the GAB are generally oligotrophic and may explain the relatively high abundance of both forage fish (sardines) and upper trophic-level predators (e.g., tuna and whales) in the region.

  6. Boundaries inside the body: women's sufferings in southern peasant Italy.

    Science.gov (United States)

    Pandolfi, M

    1990-06-01

    In a village of Southern Italy the secret world of women's emotions is fundamentally expressed through the body. The female body is open to events of the world and absorbs and feels their effect and defines a new identity, a minimal one. This gives rise to a symbolic anatomy, pathology, and physiology that serve to distinguish male and female worlds and to bridge inner and outer experience. These "traces" of external and extraordinary events, which in the past and in daily life have cut the secret and emotional world of women, are inscribed on the body. This body becomes a phenomenological memoir that opens a new way of interpreting distress and suffering and illness. This article represents a bridge from the interpretation of suffering and illness in Southern Italy via Gramsci and De Martino to a metacultural process of creating a polysemic and multilevel sense of self.

  7. Two new species of Nemesia (Scrophulariaceae from southern Africa

    Directory of Open Access Journals (Sweden)

    K. E. Steiner

    2006-12-01

    Full Text Available Two new annual species of Nemesia Vent, are described from southern Africa. N. williamsonii is characterized by bright orange flowers with an inflated yellow palate. It differs from the related N. maxii Hiern by having a spur that projects backwards, not downwards, and bracts that are lanceolate with a truncate base, not triangular or cordate. It is unusual for the genus in having dimorphic seeds.  N. williamsonii occurs almost exclusively in the Richtersveld. but has been collected in a few localities across the Orange River in southern Namibia. N. hemiptera is a delicate, wiry-stemmed annual with small white flowers. The flowers are characterized by a tiny nipple-like spur and a seed that is winged on onlv one side. N. hemiptera isendemic to the Kamiesberg from near Garies to Kamieskroon.

  8. The genus Trichodesma (Boraginaceae: Boraginoideae in southern Africa

    Directory of Open Access Journals (Sweden)

    E. Retief

    2002-12-01

    Full Text Available Trichodesma R.Br. is a genus of about 45 species known from tropical and subtropical regions of Africa. Asia and Australia. The group comprises predominantly perennial herbs, the flowers characterized by anthers with prolonged connectives, often twisting above the thecae. and a prominent accrescent calyx. Five species and three subspecies of 7rii hodesma are currently recognized in southern Africa, with  T. angustifolium Harv. subsp. argenteum Relief & A.E.van Wyk newly described. These taxa are widespread in southern Africa, occurring in various vegetation types. A key to the species, descriptions, distribution maps and illustrations of various micro- and macromorphological as well as palvnological features are provided.

  9. Origin of Quaternary Red Clay of Southern Anhui Province

    Institute of Scientific and Technical Information of China (English)

    HUXUEFENG; YUANGUODONG; 等

    1998-01-01

    The particle-size distribution,heavy mineral constituents and rare earth elements(REE) characteristics of the Quaternary red clay of southern Anhui Province were studied to explore the origin of the clay.The results showed that the clay had some properties of areolian deposits,which could be compared with,those of the loess in North China ;and its chondrite-normalized curves of REE were similar to those of the Xiashu loess implying tha they shared the same orighin.It was concluded in combination with the results rported by other researchers that the Quaternary red clay of southern Anhui Province originated from aolian deposts, and this could reveal the cycles of warm and cold climates in the area during the Quaternary period.

  10. Asymmetric auroral intensities in the Earth's Northern and Southern hemispheres

    Science.gov (United States)

    Laundal, K. M.; Østgaard, N.

    2009-07-01

    It is commonly assumed that the aurora borealis (Northern Hemisphere) and aurora australis (Southern Hemisphere) are mirror images of each other because the charged particles causing the aurora follow the magnetic field lines connecting the two hemispheres. The particles are believed to be evenly distributed between the two hemispheres, from the source region in the equatorial plane of the magnetosphere. Although it has been shown that similar auroral features in the opposite hemispheres can be displaced tens of degree in longitude and that seasonal effects can cause differences in global intensity, the overall auroral patterns were still similar. Here we report observations that clearly contradict the common assumption about symmetric aurora: intense spots are seen at dawn in the Northern summer Hemisphere, and at dusk in the Southern winter Hemisphere. The asymmetry is interpreted in terms of inter-hemispheric currents related to seasons, which have been predicted but hitherto had not been seen.

  11. ENSO effects on primary productivity in Southern Atacama desert

    Directory of Open Access Journals (Sweden)

    F. A. Squeo

    2006-01-01

    Full Text Available In the winter-rain southern Atacama Desert of the Coquimbo Region of Chile, El Niño - Southern Oscillation (ENSO events modulate primary productivity. In this region, there are important changes in water availability between La Niña (dry and El Niño (rainy years. Using inter-annual comparisons of LANDSAT images from 30° to 31° S latitude, we observed changes in primary productivity between dry and rainy years at the regional level. There were also significant, negative correlations between productivity and elevation, with changes occurring first at low elevation during rainy years. The limiting factors to dryland vegetation primary productivity is different in regard to elevation. Rain during an El Niño year is the main factor that explains the increase in primary productivity at low elevation, while lower temperatures reduce and delay the net primary productivity at mid elevation.

  12. The Economics of Bulk Water Transport in Southern California

    Directory of Open Access Journals (Sweden)

    Andrew Hodges

    2014-12-01

    Full Text Available Municipalities often face increasing demand for limited water supplies with few available alternative sources. Under some circumstances, bulk water transport may offer a viable alternative. This case study documents a hypothetical transfer between a water utility district in northern California and urban communities located on the coast of central and southern California. We compare bulk water transport costs to those of constructing a new desalination facility, which is the current plan of many communities for increasing supplies. We find that using water bags to transport fresh water between northern and southern California is in some instances a low-cost alternative to desalination. The choice is constrained, however, by concerns about reliability and, thus, risk. Case-study results demonstrate the challenges of water supply augmentation in water-constrained regions.

  13. Tuberculosis Treatment Completion Rates in Southern New Mexico Colonias.

    Science.gov (United States)

    Holden, Maria Arroyo; Huttlinger, Kathleen; Schultz, Pamela; Mullins, Iris; Forster-Cox, Sue

    2016-04-01

    TB medication completion treatment rates for active TB patients living in impoverished US-Mexico border communities called colonias in southern New Mexico counties are unknown. It might be suspected that residents of colonias have lower completion rates than those living in incorporated and medically more accessible areas. A retrospective record review of closed TB case records from 1993 to 2010 of southern New Mexico border counties, was conducted using a modified version of the New Mexico Department of Health Tuberculosis Targeted Health Assessment/History form (Appendix 1). Study findings reveal that despite their unincorporated status, poorer living conditions and questionable legal status, colonia TB patients had a higher medication completion rate than their non-colonia counterparts. A robust New Mexico TB treatment program contributed to high completion rates with death being the number-one reason for treatment non-completion in both colonia and non-colonias.

  14. Annual Incidence of Lassa Virus Infection in Southern Mali.

    Science.gov (United States)

    Safronetz, David; Sogoba, Nafomon; Diawara, Sory Ibrahim; Bane, Sidy; Rosenke, Kyle; Maiga, Ousmane; Boisen, Matt; Garry, Robert F; Branco, Luis M; Lindsay, L Robbin; Traoré, Sékou F; Feldmann, Heinz; Doumbia, Seydou

    2017-01-16

    Previously, we reported a high seroprevalence rate of Lassa virus antibodies in inhabitants of three villages in southern Mali where infected rodents have been demonstrated. Herein, we report a 1-year follow-up study in which we were able to collect a second blood samples from 88.7% of participants of the same cohort. We identified 23 seroconversions for IgG antibodies reactive against Lassa virus, representing an incidence of 6.3% (95% confidence interval = 3.8-8.8%). Seroconversion was frequently seen in preteenage children (12/23, 51.7%) and two household/familial clusters were identified. These results confirm active transmission of Lassa virus is occurring in southern Mali and appropriate diagnostic testing should be established for this often severe etiological agent viral hemorrhagic fever.

  15. Interannual variability of the southern Yellow Sea Cold Water Mass

    Institute of Scientific and Technical Information of China (English)

    胡敦欣; 王庆业

    2004-01-01

    Temperature data collected in the sections of 34°N, 35°N and 36°N in August from 1975 through2003 were analyzed using Empirical Orthogonal Function (EOF) to investigate interannual variability of the southern Yellow Sea Cold Water Mass (YSCWM). The first mode (EOF1) reveals variations of basin-wide thermocline depth, which is mainly caused by surface heating. The second mode (EOF2) presents fluctuations of vertical circulation, resulting mainly from interannual variability of cold front intensity. In addition, it is found that the upward extent of upwelling in the cold front is basically determined by wind stress curl and the zonal position of the warm water center in the southern Yellow Sea is correlated with spatial difference of net heat flux.

  16. The Place of Rural in a Southern Criminology

    Directory of Open Access Journals (Sweden)

    Joseph Francis Donnermeyer

    2017-03-01

    Full Text Available A substantial proportion of the world’'s population remains rural, despite decades of urbanisation. Further, most of this rural population lies south of the equator. Therefore, it is incumbent on the emerging fields of rural criminology and global southern criminology to mutually reinforce each other’'s scholarly development. To this end, this article engages three selected issues associated with agriculture and food – - agricultural victimisation, food security, and farmworker abuse and trafficking –- and discusses them in terms of the advancement of a global southern criminology. The article concludes with acknowledgement of many more rural crime issues that have particular salience to the global South and warns of new dangers in the development of hegemonic binaries (Carrington, Hogg and Sozzo 2016 and homogenous categories of knowledge (Connell 2007 if they fail to inform each other.

  17. Micronutrient Contents of Citrus Orchard Soils in Southern China

    Institute of Scientific and Technical Information of China (English)

    OUYANGTAO

    1993-01-01

    Investigations were carried out on the micronutrient contents,of major citrus orchard soils (involving seven soil great groups in 8 provinces and an autonomous region of southern China),and an evaluation on the abundance and deficiency of available micronutrients in these soils was made in this paper.In southern China,citrus orchard soils derived from sandstone,sandy shale,Quaternary red clay,diluvial deposit,granite gneiss and neritic deposit were deficient in available Mo and B and low in Zn.Those developed on purple sandy shale,limestone and slope deposit were all in short supply of available Zn,B and Mo.Coastal solonchak was fairly abundant in B,but its available Fe,Zn and Mo contents were rather low.

  18. Challenges and opportunities for Korean missionaries in Southern Africa

    Directory of Open Access Journals (Sweden)

    Kyung H. Oh

    2009-03-01

    Full Text Available This article describes the history of missions in Korea as well as the challenges and opportunities for Korean missionaries in Southern Africa. The most significant problems encountered by Korean missionaries include understanding local context, language and culture acquisition, and meeting the expectations of local people and local churches as well as those of sending churches in Korea. On a personal level, missionaries have to cope with family concerns, maintaining their spiritual life, health problems, financial concerns, frustrations and unfulfilled ideals. Korean missionaries in Southern Africa do, however, have a unique opportunity to serve the Church in its mission and, above all, to serve the Lord of the Church in his mission, although there are indeed difficulties to overcome and challenges to face.

  19. Retrospective study of prevalence of face fractures in southern Brazil

    OpenAIRE

    Jefferson Viapiana Paes; Franciana Lima de Sá Paes; Renato Valiati; Marília G Oliveira; Rogério Miranda Pagnoncelli

    2012-01-01

    Context: Trauma has been an important public health problem worldwide. Facial injuries are among the most common types of trauma treated at emergency departments, associated or not with injuries in other anatomic sites. The patterns of facial fractures are usually affected by geography and socioeconomic conditions. Aim: To investigate the prevalence of facial fractures in Lages, state of Santa Catarina, southern Brazil, from September 2003 to August 2008. Settings and Design: Cross-se...

  20. Continental Airlines and China Southern Airlines Announce Strategic Partnership

    Institute of Scientific and Technical Information of China (English)

    2007-01-01

    @@ Frequent flyer reciprocity to start in September: codesharing to start in November. Continental Airlines and China Southern Airlines. the largest airline in the People's Republic of China. announced on June 18, 2007 that they have entered into a strategic partnership for frequent flyer and airport lounge access reciprocity and extensive codesharing, providing better service to customers who travel between the U.S.and China and transfer between the two carriers.